WorldWideScience

Sample records for active rack isolation

  1. Seismic testing of the base-isolated PWR spent-fuel storage rack

    International Nuclear Information System (INIS)

    Fujita, Katsuhisa; Tanaka, Mamoru; Nakamura, Masaaki; Tsujikura, Yonezo.

    1990-01-01

    The present paper aims to verify the seismic safety of the base-isolated spent-fuel storage rack. A series of seismic tests has been conducted using a three-dimensional shaking table. A sliding-type base-isolation system was employed for the prototype rack considering environmental conditions in an actual plant. A non linear seismic response analysis was also performed, and it is verified that the prototype of a base-isolated spent-fuel storage rack has a sufficient seismic safety margin for design seismic conditions from the viewpoint of seismic response. (author)

  2. FSI-based Overflow Assessment of the Seismically-Isolated SFP with Fuel Racks

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Gil Y.; Park, Hyun T.; Chang, Soo-Hyuk [Korea Maintenance Co., Seoul (Korea, Republic of); Lee, Sang-Hoon [KEPCO E-C, Yongin (Korea, Republic of)

    2014-10-15

    To date, effectiveness of the seismic isolation systems for reducing seismic force effectively has been well demonstrated. In this context, practical application of the technology in nuclear engineering fields has become an important issue more and more. This is because fluid motion can be rather amplified due to the increased relative displacement between the base and superstructures by a long-period shift. Therefore, overflow assessment and prediction of the seismically-isolated SFP have to be conducted in design phase. For performing sloshing-induced overflow of the seismically-isolated SFP, a fluid-structure interaction(FSI) approach making a two-way coupling process between structural and fluid solvers is herein employed. In this study, fuel racks inside the SFP are included in FSI modeling to investigate effect of fuel-cell assemblies on SFP overflow. Accordingly, three different assembly sets of fuel cells are assumed to be inserted in fuel racks. In addition, floor acceleration time-histories produced from three different amplitudes of peak ground acceleration (PGA) are applied to the SFP base to investigate load effect on liquid overflow. An approach for the liquid overflow assessment of the seismically-isolated nuclear SFP with fuel storage racks based on FSI analysis was addressed. From the results of the identified cases, the following conclusions are drawn: (i) FSI technique can be effectively used to assess the seismically-isolated SFP overflow, (ii) In a conservative way, the isolated SFP without fuel racks can be used to assess its sloshing-induced overflow under earthquake since effect of fuel-cell assemblies on the SFP overflow is not significant, (iii) for given same conditions (e.g., constant design free surface, same fuel-cell assembly) except seismic loading, the higher PGA is, the more liquid overflow increases.

  3. Depletion of WRN protein causes RACK1 to activate several protein kinase C isoforms

    DEFF Research Database (Denmark)

    Massip, L; Garand, C; Labbé, A

    2010-01-01

    show that a knock down of the WRN protein in normal human fibroblasts induces phosphorylation and activation of several protein kinase C (PKC) enzymes. Using a tandem affinity purification strategy, we found that WRN physically and functionally interacts with receptor for activated C-kinase 1 (RACK1......), a highly conserved anchoring protein involved in various biological processes, such as cell growth and proliferation. RACK1 binds strongly to the RQC domain of WRN and weakly to its acidic repeat region. Purified RACK1 has no impact on the helicase activity of WRN, but selectively inhibits WRN exonuclease...... activity in vitro. Interestingly, knocking down RACK1 increased the cellular frequency of DNA breaks. Depletion of the WRN protein in return caused a fraction of nuclear RACK1 to translocate out of the nucleus to bind and activate PKCdelta and PKCbetaII in the membrane fraction of cells. In contrast...

  4. Characterization of receptor of activated C kinase 1 (RACK1) and functional analysis during larval metamorphosis of the oyster Crassostrea angulata.

    Science.gov (United States)

    Yang, Bingye; Pu, Fei; Qin, Ji; You, Weiwei; Ke, Caihuan

    2014-03-10

    During a large-scale screen of the larval transcriptome library of the Portuguese oyster, Crassostrea angulata, the oyster gene RACK, which encodes a receptor of activated protein kinase C protein was isolated and characterized. The cDNA is 1,148 bp long and has a predicted open reading frame encoding 317 aa. The predicted protein shows high sequence identity to many RACK proteins of different organisms including molluscs, fish, amphibians and mammals, suggesting that it is conserved during evolution. The structural analysis of the Ca-RACK1 genomic sequence implies that the Ca-RACK1 gene has seven exons and six introns, extending approximately 6.5 kb in length. It is expressed ubiquitously in many oyster tissues as detected by RT-PCR analysis. The Ca-RACK1 mRNA expression pattern was markedly increased at larval metamorphosis; and was further increased along with Ca-RACK1 protein synthesis during epinephrine-induced metamorphosis. These results indicate that the Ca-RACK1 plays an important role in tissue differentiation and/or in cell growth during larval metamorphosis in the oyster, C. angulata. Copyright © 2013 Elsevier B.V. All rights reserved.

  5. Dioxin modulates expression of receptor for activated C kinase (RACK-1) in developing neurons

    Energy Technology Data Exchange (ETDEWEB)

    Yang, J.H.; Kim, S.Y.; Lee, H.G.; Kim, M.Y.; Lee, J.H.; Chae, W.G. [Catholic Univ. of Daegu, Dept. of Pharmacology/Toxicology, Daegu (Korea)

    2004-09-15

    TCDD is sensitive to the central nerve system of the developing brain. The TCDD-induced neurodevelopmental deficits include the cognitive disability and motor dysfunction. While TCDD may lead to neurodevelopmental and neurobehavioral deficit, it is not known which molecular substances are intracellular targets for TCDD. Since TCDD accumulates in brain and the brain contains the Ah receptor, it is possible that TCDD may act at the target site such as cerebellum, which is responsible for cognitive abilities and motor function. A recent in vitro studies using cerebellar granule cells demonstrated a translocation of PKC-{alpha} and {epsilon} following the TCDD or PCB exposure. One of the most pivotal second messenger molecules involved in neuronal function and development is protein kinase C (PKC). PKC signaling pathways have been implicated as an important factor in learning and memory processes. PKC signaling events are optimized by the adaptor proteins, which organize PKCs near their selective substrates and away from others. RACK-1(receptor for activated C-kinase) is one of adaptor proteins that anchor the activated PKC at the site of translocation 6. RACKs bind PKC only in the presence of PKC activators. RACKs are 30- and 36-kDa proteins located in cytoskeletal compartment and play a key role in PKC activation and in membrane amchoring. Since different PKC isoforms translocate to distinct subcellular sites on activation, it is suggested that isoform-specific RACK may be present. Activation of certain PKC isoforms (PKC-a and {beta}II) is preferentially associated with RACK-1. While TCDD modulates PKC signaling pathway, role of RACK-1 on TCDD-mediated signaling pathway is not known. To identify the intracellular target for TCDD and understand a mechanism of signaling pathway in the developing brain, the present study attempted to analyze effects of RACK-1 in the cerebellar granule cells following TCDD exposure.

  6. Financial aspects of a seismic base isolation system for a steel high-rack structure

    Directory of Open Access Journals (Sweden)

    Kilar, V.

    2013-12-01

    Full Text Available The paper deals with the effects and costs of implementing a base isolation system for the mitigation of the seismic risk of an existing steel rack structure. Different realistic distributions of the payload mass and occupancy levels, which form different plan asymmetric variants, have been analysed. The results obtained by the pushover analysis (N2 method are presented as top floor envelopes and as plastic hinge damage patterns. In the presented cost study, the cost of the implementation of the proposed base isolation system is compared with the estimated costs of structural repairs to the damaged structural members of the superstructure, as well as with estimated expenses of the downtime period. The results have shown that base isolation is, in general, not economically feasible for lower ground motion intensities, whereas it could be of great benefit in the case of moderate and high intensities, especially if the downtime period is taken into account.El presente artículo trata sobre los efectos y costes de implementación de un sistema de aislamiento en cimentación para la mitigación del riesgo sísmico de la estructura de un bastidor de acero en altura prexistente. Se han analizado diferentes distribuciones realistas de la masa contribuyente y de los niveles de ocupación, conformando diferentes variantes asimétricas en planta. Se presentan los resultados obtenidos mediante el método N2 (análisis estático incremental no lineal como envolventes de las plantas superiores y como patrones de deterioro en estado plástico. En el estudio de costos presentado, el coste de implementación del sistema de aislamiento propuesto se compara con los costes estimados de reparación de los elementos superestructurales y los costes derivados del período de desocupación. Los resultados muestran que, en general, el aislamiento en la base no resulta viable económicamente para movimientos de baja intensidad, pero puede ser muy beneficioso en el caso de

  7. Conducting Research on the International Space Station using the EXPRESS Rack Facilities

    Science.gov (United States)

    Thompson, Sean W.; Lake, Robert E.

    2016-01-01

    Eight "Expedite the Processing of Experiments to Space Station" (EXPRESS) Rack facilities are located within the International Space Station (ISS) laboratories to provide standard resources and interfaces for the simultaneous and independent operation of multiple experiments within each rack. Each EXPRESS Rack provides eight Middeck Locker Equivalent locations and two drawer locations for powered experiment equipment, also referred to as sub-rack payloads. Payload developers may provide their own structure to occupy the equivalent volume of one, two, or four lockers as a single unit. Resources provided for each location include power (28 Vdc, 0-500 W), command and data handling (Ethernet, RS-422, 5 Vdc discrete, +/- 5 Vdc analog), video (NTSC/RS 170A), and air cooling (0-200 W). Each rack also provides water cooling for two locations (500W ea.), one vacuum exhaust interface, and one gaseous nitrogen interface. Standard interfacing cables and hoses are provided on-orbit. One laptop computer is provided with each rack to control the rack and to accommodate payload application software. Four of the racks are equipped with the Active Rack Isolation System to reduce vibration between the ISS and the rack. EXPRESS Racks are operated by the Payload Operations Integration Center at Marshall Space Flight Center and the sub-rack experiments are operated remotely by the investigating organization. Payload Integration Managers serve as a focal to assist organizations developing payloads for an EXPRESS Rack. NASA provides EXPRESS Rack simulator software for payload developers to checkout payload command and data handling at the development site before integrating the payload with the EXPRESS Functional Checkout Unit for an end-to-end test before flight. EXPRESS Racks began supporting investigations onboard ISS on April 24, 2001 and will continue through the life of the ISS.

  8. Materials Science Research Rack-1 Fire Suppressant Distribution Test Report

    Science.gov (United States)

    Wieland, P. O.

    2002-01-01

    Fire suppressant distribution testing was performed on the Materials Science Research Rack-1 (MSRR-1), a furnace facility payload that will be installed in the U.S. Lab module of the International Space Station. Unlike racks that were tested previously, the MSRR-1 uses the Active Rack Isolation System (ARIS) to reduce vibration on experiments, so the effects of ARIS on fire suppressant distribution were unknown. Two tests were performed to map the distribution of CO2 fire suppressant throughout a mockup of the MSRR-1 designed to have the same component volumes and flowpath restrictions as the flight rack. For the first test, the average maximum CO2 concentration for the rack was 60 percent, achieved within 45 s of discharge initiation, meeting the requirement to reach 50 percent throughout the rack within 1 min. For the second test, one of the experiment mockups was removed to provide a worst-case configuration, and the average maximum CO2 concentration for the rack was 58 percent. Comparing the results of this testing with results from previous testing leads to several general conclusions that can be used to evaluate future racks. The MSRR-1 will meet the requirements for fire suppressant distribution. Primary factors that affect the ability to meet the CO2 distribution requirements are the free air volume in the rack and the total area and distribution of openings in the rack shell. The length of the suppressant flowpath and degree of tortuousness has little correlation with CO2 concentration. The total area of holes in the rack shell could be significantly increased. The free air volume could be significantly increased. To ensure the highest maximum CO2 concentration, the PFE nozzle should be inserted to the stop on the nozzle.

  9. An embedded acceleration measurement capability for EXPRESS Rack Payloads

    International Nuclear Information System (INIS)

    Foster, William M. II; Sutliff, Thomas J.

    2000-01-01

    The International Space Station provides a microgravity environment allowing long duration studies to be made on phenomena masked by the presence of earth's gravitational effects. Studies are also enabled in areas requiring a substantial decrease in steady-state and vibratory acceleration environments. In anticipation microgravity science experiments being targeted for EXPRESS (EXpedite the PRocessing of Experiments to Space Station) Racks, a capability has been provided to simplify and conduct a consistent measurement of the microgravity environment for payloads. The Space Acceleration Measurement System-II (SAMS-II) project has collaborated with the EXPRESS Rack Project to embed an electronics unit within the four EXPRESS Racks equipped with Active Rack Isolation Systems (ARIS). Each SAMS-II unit provides a standardized means for payload acceleration measurements to be acquired. Access to this capability is via front panel connections similar to those of power, data and water cooling provided for EXPRESS payloads. Furthermore, an International Subrack Interface Standard (ISIS) drawer configuration has been developed to provide measurement capability to the non-ARIS equipped EXPRESS Racks, as well as to other ISIS-configured racks, for non-isolated experimental measurement needs. This paper describes the SAMS-II acceleration measurement capabilities provided to ISS users and, in particular, to the EXPRESS Rack community

  10. The NRC activities concerning Boraflex use in spent-fuel storage racks

    International Nuclear Information System (INIS)

    Kopp, L.I.

    1996-01-01

    The U.S. Nuclear Regulatory Commission (NRC) has issued several generic communications to the nuclear industry identifying two issues with respect to using Boraflex in spent-fuel storage racks. The first issue related to gamma-radiation-induced shrinkage of Boraflex and the potential to develop tears or gaps in the material. This phenomenon is typically accounted for in criticality analyses of spent-fuel storage racks. The second issue concerned long-term Boraflex performance throughout the intended service life of the racks as a result of both gamma irradiation and exposure to the wet pool environment

  11. Design Features and Capabilities of the First Materials Science Research Rack

    Science.gov (United States)

    Pettigrew, P. J.; Lehoczky, S. L.; Cobb, S. D.; Holloway, T.; Kitchens, L.

    2003-01-01

    The First Materials Science Research Rack (MSRR-1) aboard the International Space Station (ISS) will offer many unique capabilities and design features to facilitate a wide range of materials science investigations. The initial configuration of MSRR-1 will accommodate two independent Experiment Modules (EMS) and provide the capability for simultaneous on-orbit processing. The facility will provide the common subsystems and interfaces required for the operation of experiment hardware and accommodate telescience capabilities. MSRR1 will utilize an International Standard Payload Rack (ISPR) equipped with an Active Rack Isolation System (ARIS) for vibration isolation of the facility.

  12. Recalculation with SEACAB of the activation by spent fuel neutrons and residual dose originated in the racks replaced at Cofrentes NPP

    Directory of Open Access Journals (Sweden)

    Ortego Pedro

    2017-01-01

    Full Text Available In order to increase the storage capacity of the East Spent Fuel Pool at the Cofrentes NPP, located in Valencia province, Spain, the existing storage stainless steel racks were replaced by a new design of compact borated stainless steel racks allowing a 65% increase in fuel storing capacity. Calculation of the activation of the used racks was successfully performed with the use of MCNP4B code. Additionally the dose rate at contact with a row of racks in standing position and behind a wall of shielding material has been calculated using MCNP4B code as well. These results allowed a preliminary definition of the burnker required for the storage of racks. Recently the activity in the racks has been recalculated with SEACAB system which combines the mesh tally of MCNP codes with the activation code ACAB, applying the rigorous two-step method (R2S developed at home, benchmarked with FNG irradiation experiments and usually applied in fusion calculations for ITER project.

  13. Recalculation with SEACAB of the activation by spent fuel neutrons and residual dose originated in the racks replaced at Cofrentes NPP

    Science.gov (United States)

    Ortego, Pedro; Rodriguez, Alain; Töre, Candan; Compadre, José Luis de Diego; Quesada, Baltasar Rodriguez; Moreno, Raul Orive

    2017-09-01

    In order to increase the storage capacity of the East Spent Fuel Pool at the Cofrentes NPP, located in Valencia province, Spain, the existing storage stainless steel racks were replaced by a new design of compact borated stainless steel racks allowing a 65% increase in fuel storing capacity. Calculation of the activation of the used racks was successfully performed with the use of MCNP4B code. Additionally the dose rate at contact with a row of racks in standing position and behind a wall of shielding material has been calculated using MCNP4B code as well. These results allowed a preliminary definition of the burnker required for the storage of racks. Recently the activity in the racks has been recalculated with SEACAB system which combines the mesh tally of MCNP codes with the activation code ACAB, applying the rigorous two-step method (R2S) developed at home, benchmarked with FNG irradiation experiments and usually applied in fusion calculations for ITER project.

  14. LIE n-RACKS

    OpenAIRE

    Biyogmam, Guy Roger

    2011-01-01

    In this paper, we introduce the category of Lie $n$-racks and generalize several results known on racks. In particular, we show that the tangent space of a Lie $n$-Rack at the neutral element has a Leibniz $n$-algebra structure. We also define a cohomology theory of $n$-racks..

  15. Crossed modules of racks

    OpenAIRE

    Crans, Alissa S.; Wagemann, Friedrich

    2014-01-01

    We generalize the notion of a crossed module of groups to that of a crossed module of racks. We investigate the relation to categorified racks, namely strict 2-racks, and trunk-like objects in the category of racks, generalizing the relation between crossed modules of groups and strict 2-groups. Then we explore topological applications. We show that by applying the rack-space functor, a crossed module of racks gives rise to a covering. Our main result shows how the fundamental racks associate...

  16. Reusable Rack Interface Controller Common Software for Various Science Research Racks on the International Space Station

    Science.gov (United States)

    Lu, George C.

    2003-01-01

    The purpose of the EXPRESS (Expedite the PRocessing of Experiments to Space Station) rack project is to provide a set of predefined interfaces for scientific payloads which allow rapid integration into a payload rack on International Space Station (ISS). VxWorks' was selected as the operating system for the rack and payload resource controller, primarily based on the proliferation of VME (Versa Module Eurocard) products. These products provide needed flexibility for future hardware upgrades to meet everchanging science research rack configuration requirements. On the International Space Station, there are multiple science research rack configurations, including: 1) Human Research Facility (HRF); 2) EXPRESS ARIS (Active Rack Isolation System); 3) WORF (Window Observational Research Facility); and 4) HHR (Habitat Holding Rack). The RIC (Rack Interface Controller) connects payloads to the ISS bus architecture for data transfer between the payload and ground control. The RIC is a general purpose embedded computer which supports multiple communication protocols, including fiber optic communication buses, Ethernet buses, EIA-422, Mil-Std-1553 buses, SMPTE (Society Motion Picture Television Engineers)-170M video, and audio interfaces to payloads and the ISS. As a cost saving and software reliability strategy, the Boeing Payload Software Organization developed reusable common software where appropriate. These reusable modules included a set of low-level driver software interfaces to 1553B. RS232, RS422, Ethernet buses, HRDL (High Rate Data Link), video switch functionality, telemetry processing, and executive software hosted on the FUC computer. These drivers formed the basis for software development of the HRF, EXPRESS, EXPRESS ARIS, WORF, and HHR RIC executable modules. The reusable RIC common software has provided extensive benefits, including: 1) Significant reduction in development flow time; 2) Minimal rework and maintenance; 3) Improved reliability; and 4) Overall

  17. Spent fuel storage rack

    International Nuclear Information System (INIS)

    Morikawa, Matsuo; Uchiyama, Yuichi.

    1983-01-01

    Purpose: To improve the safety and facilitate the design by limiting the relative displacement in a storage rack. Constitution: The outer wall of a storage rack disposed in water within a fuel pool, the pool wall opposing to the storage rack and the structure between the opposing storages racks are made as a space for confining the pool water or a structure formed with a slight gap, for example, a combination of a recessed structure and a protruded structure. In such a constitution, a space for confirming the pool water is established and the pool water thus confined forms a flow resistance when the storage rack vibrates upon earthquakes, serves as a damper and significantly reduces the responsivity. Furthermore, the relative displacement in the storage rack is limited to inhibit excess earthquake forces to exert on setting bolts and rack clamping bolts of the storage rack. (Sekiya, K.)

  18. Rotating specimen rack repair

    International Nuclear Information System (INIS)

    Miller, G.E.; Rogers, P.J.; Nabor, W.G.; Bair, H.

    1984-01-01

    In 1980, an operator at the UCI TRIGA Reactor noticed difficulties with the rotation of the specimen rack. Investigations showed that the drive bearing in the rack had failed and allowed the bearings to enter the rack. After some time of operation in static mode it was decided that installation of a bearing substitute - a graphite sleeve - would be undertaken. Procedures were written and approved for removal of the rack, fabrication and installation of the sleeve, and re-installation of the rack. This paper describes these procedures in some detail. Detailed drawings of the necessary parts may be obtained from the authors

  19. MSRR Rack Materials Science Research Rack

    Science.gov (United States)

    Reagan, Shawn

    2017-01-01

    The Materials Science Research Rack (MSRR) is a research facility developed under a cooperative research agreement between NASA and the European Space Agency (ESA) for materials science investigations on the International Space Station (ISS). The MSRR is managed at the Marshall Space Flight Center (MSFC) in Huntsville, AL. The MSRR facility subsystems were manufactured by Teledyne Brown Engineering (TBE) and integrated with the ESA/EADS-Astrium developed Materials Science Laboratory (MSL) at the MSFC Space Station Integration and Test Facility (SSITF) as part of the Systems Development Operations Support (SDOS) contract. MSRR was launched on STS-128 in August 2009, and is currently installed in the U. S. Destiny Laboratory Module on the ISS. Materials science is an integral part of developing new, safer, stronger, more durable materials for use throughout everyday life. The goal of studying materials processing in space is to develop a better understanding of the chemical and physical mechanisms involved, and how they differ in the microgravity environment of space. To that end, the MSRR accommodates advanced investigations in the microgravity environment of the ISS for basic materials science research in areas such as solidification of metals and alloys. MSRR allows for the study of a variety of materials including metals, ceramics, semiconductor crystals, and glasses. Materials science research benefits from the microgravity environment of space, where the researcher can better isolate chemical and thermal properties of materials from the effects of gravity. With this knowledge, reliable predictions can be made about the conditions required on Earth to achieve improved materials. MSRR is a highly automated facility with a modular design capable of supporting multiple types of investigations. Currently the NASA-provided Rack Support Subsystem provides services (power, thermal control, vacuum access, and command and data handling) to the ESA developed Materials

  20. EXPRESS Rack Technology for Space Station

    Science.gov (United States)

    Davis, Ted B.; Adams, J. Brian; Fisher, Edward M., Jr.; Prickett, Guy B.; Smith, Timothy G.

    1999-01-01

    The EXPRESS rack provides accommodations for standard Mid-deck Locker and ISIS drawer payloads on the International Space Station. A design overview of the basic EXPRESS rack and two derivatives, the Human Research Facility and the Habitat Holding Rack, is given in Part I. In Part II, the design of the Solid State Power Control Module (SSPCM) is reviewed. The SSPCM is a programmable and remotely controllable power switching and voltage conversion unit which distributes and protects up to 3kW of 12OVDC and 28VDC power to payloads and rack subsystem components. Part III details the development and testing of a new data storage device, the BRP EXPRESS Memory Unit (BEMU). The BEMU is a conduction-cooled device which operates on 28VDC and is based on Boeing-modified 9GB commercial disk-drive technology. In Part IV results of a preliminary design effort for a rack Passive Damping System (PDS) are reported. The PDS is intended to isolate ISPR-based experiment racks from on-orbit vibration. System performance predictions based on component developmental testing indicate that such a system can provide effective isolation at frequencies of 1 Hz and above.

  1. Flow in data racks

    Directory of Open Access Journals (Sweden)

    Manoch Lukáš

    2014-03-01

    Full Text Available This paper deals with the flow in data racks. The aim of this work is to find a new arrangement of elements regulating the flow in the data rack so that the aerodynamic losses and the recirculation zones were minimized. The main reason for solving this problem is to reduce the costs of data racks cooling. Another problem to be solved is a reverse flow in the servers, thus not cooled, occuring due to the underpressure in the recirculation zones. In order to solve the problem, the experimental and numerical model of 27U data rack fitted with 10 pieces of server models with a total input of 10 kW was created. Different configurations of layout of elements affecting the flow in the inlet area of the data rack were compared. Depending on the results achieved, design solutions for the improvement of existing solutions were adopted and verified by numerical simulations.

  2. Thesaurus Racks - Categorical racks and applications in the algebraic topology of Lie racks

    OpenAIRE

    Grøsfjeld, Tobias

    2016-01-01

    Group objects of categories have been heavily studied in a general setting, but racks are mostly treated explicitly. Since rack structures are more general than groups, this thesis aims to explore the properties of general rack objects and use the tools of category theory to put topological racks in a new light.

  3. Fuel assembly storing rack

    International Nuclear Information System (INIS)

    Kajimura, Haruhiko; Nakamura, Masaaki.

    1997-01-01

    In a nuclear fuel storage rack comprising a plurality of square-shaped rack cells arranged vertically in a lattice-like configuration, the square rack cell comprises a stainless steel having a boron content of 0.75% or less and a boron equivalent with respect to calculated thermal neutron absorbing performance of 1.0 or more, and a distance between each of the gap of the square rack cells in adjacent with each other satisfies a predetermined condition. One example of the content is that B is from 0.05 to 0.75%, Gd is from 0.05 to 1.50%, C is 0.03% or less, Si is 1.0% or less, Mn is from 0.1 to 2.0%, P is 0.03% or less, S is 0.01% or less, Cr is from 18 to 26%, Ni is from 7 to 22% and Al is 0.1% or less. The distance between the rack cells can be reduced by using a material improved with thermal neutron absorbing performance by determining the boron equivalent to a predetermined value or more and with less B-content and good fabricability. In addition, the size of the rack cell itself can be reduced. This can greatly reduce the nuclear fuel storage area. (I.S.)

  4. First Materials Science Research Facility Rack Capabilities and Design Features

    Science.gov (United States)

    Cobb, S.; Higgins, D.; Kitchens, L.; Curreri, Peter (Technical Monitor)

    2002-01-01

    The first Materials Science Research Rack (MSRR-1) is the primary facility for U.S. sponsored materials science research on the International Space Station. MSRR-1 is contained in an International Standard Payload Rack (ISPR) equipped with the Active Rack Isolation System (ARIS) for the best possible microgravity environment. MSRR-1 will accommodate dual Experiment Modules and provide simultaneous on-orbit processing operations capability. The first Experiment Module for the MSRR-1, the Materials Science Laboratory (MSL), is an international cooperative activity between NASA's Marshall Space Flight Center (MSFC) and the European Space Agency's (ESA) European Space Research and Technology Center (ESTEC). The MSL Experiment Module will accommodate several on-orbit exchangeable experiment-specific Module Inserts which provide distinct thermal processing capabilities. Module Inserts currently planned for the MSL are a Quench Module Insert, Low Gradient Furnace, and a Solidification with Quench Furnace. The second Experiment Module for the MSRR-1 configuration is a commercial device supplied by MSFC's Space Products Development (SPD) Group. Transparent furnace assemblies include capabilities for vapor transport processes and annealing of glass fiber preforms. This Experiment Module is replaceable on-orbit. This paper will describe facility capabilities, schedule to flight and research opportunities.

  5. Solution structure of the human signaling protein RACK1

    Directory of Open Access Journals (Sweden)

    Papa Priscila F

    2010-06-01

    Full Text Available Abstract Background The adaptor protein RACK1 (receptor of activated kinase 1 was originally identified as an anchoring protein for protein kinase C. RACK1 is a 36 kDa protein, and is composed of seven WD repeats which mediate its protein-protein interactions. RACK1 is ubiquitously expressed and has been implicated in diverse cellular processes involving: protein translation regulation, neuropathological processes, cellular stress, and tissue development. Results In this study we performed a biophysical analysis of human RACK1 with the aim of obtaining low resolution structural information. Small angle X-ray scattering (SAXS experiments demonstrated that human RACK1 is globular and monomeric in solution and its low resolution structure is strikingly similar to that of an homology model previously calculated by us and to the crystallographic structure of RACK1 isoform A from Arabidopsis thaliana. Both sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation techniques showed that RACK1 is predominantly a monomer of around 37 kDa in solution, but also presents small amounts of oligomeric species. Moreover, hydrodynamic data suggested that RACK1 has a slightly asymmetric shape. The interaction of RACK1 and Ki-1/57 was tested by sedimentation equilibrium. The results suggested that the association between RACK1 and Ki-1/57(122-413 follows a stoichiometry of 1:1. The binding constant (KB observed for RACK1-Ki-1/57(122-413 interaction was of around (1.5 ± 0.2 × 106 M-1 and resulted in a dissociation constant (KD of (0.7 ± 0.1 × 10-6 M. Moreover, the fluorescence data also suggests that the interaction may occur in a cooperative fashion. Conclusion Our SAXS and analytical ultracentrifugation experiments indicated that RACK1 is predominantly a monomer in solution. RACK1 and Ki-1/57(122-413 interact strongly under the tested conditions.

  6. Spent fuel storage rack

    International Nuclear Information System (INIS)

    Kurokawa, Hideaki; Kumagaya, Naomi; Oda, Masashi; Matsuda, Masami; Maruyama, Hiromi; Yamanaka, Tsuneyasu.

    1997-01-01

    The structure of a spent fuel storage rack is determined by the material, thickness, size of square cylindrical tubes (the gap between spent fuel assemblies and the square cylindrical tubes) and pitch of the arrangement (the gap between each of the square cylindrical tubes). In the present invention, the thickness and the pitch of the arrangement of the square tubes are optimized while evaluating subcriticality. Namely, when the sum of the thickness of the water gap at the outer side (the pitch of arrangement of the cylindrical tubes) and the thickness of the cylindrical tubes is made constant, the storage rack is formed by determining the thickness of the cylindrical tubes which is smaller than the optimum value among the combination of the thickness of the water gap at the outer side and that of the cylindrical tube under the effective multiplication factor to be performed. Then, the weight of the rack can be reduced, and the burden of the load on the bottom of the pool can be reduced. Further, the amount of the constitutional materials of the rack itself can be reduced thereby capable of reducing the cost for the materials of the rack. (T.M.)

  7. Seismic analysis of rack structures for fuel cycle facilities

    International Nuclear Information System (INIS)

    Mochio, Takashi; Morooka, Akihiko; Ito, Takashi.

    1987-01-01

    A concept of remote maintenance using in large remote cell and rack system structure, which is now under development at high active liquid waste vitrification facility of PNC and West Germany reprocessing plant WA-350, has been adopted to reduce the radiation exposure and increase the operating efficiency. The operation of a highly efficient remote maintenance system sometimes requires the rack structures to be fairly flexible, because of the large number of loose connections and/or gapped supports and the low number of rack frames. This means that there is a possibility of severe damage occurring due to large amplitude responses during a strong earthquake. Therefore, it is very important to estimate the earthquake-resistance capacity of rack structures, including process equipment, to earthquake excitation. This paper presents an outline of a new computer code ''FRACK'' to analyze the nonlinear seismic response of a rack structure developed as a first stage in the rack system seismic research program. (author)

  8. Staphylococcus xylosus PCR-validated Decontamination of Murine Individually Ventilated Cage Racks and Air Handling Units by Using 'Active-Closed' Exposure to Vaporized Hydrogen Peroxide.

    Science.gov (United States)

    Ragland, Natalie H; Miedel, Emily L; Gomez, Jose M; Engelman, Robert W

    2017-11-01

    Vaporized hydrogen peroxide (VHP) is used to decontaminate clinical, biocontainment, and research animal rooms and equipment. To assist with its implementation in a murine facility, we developed a safe and effective method of VHP sterilization of IVC racks and air handling units (AHU). Safety of VHP decontamination was assessed by ensuring VHP levels dissipated to less than 1 ppm in the room prior to personnel reentry and inside the primary enclosure prior to the return of mice; this condition occurred at least 18 h after the VHP cycle. Efficacy of VHP sterilization was assessed by using chemical indicators, biologic indicators, and PCR testing for Staphylococcus xylosus, a commensal organism of murine skin and an opportunistic pathogen, which was present in 160 of 172 (93%) of specimens from occupied IVC racks and the interior surfaces of in-use AHU. Neither mechanized washing nor hand-sanitizing eradicated S. xylosus from equipment airway interiors, with 17% to 24% of specimens remaining PCR-positive for S. xylosus. 'Static-open' VHP exposure of sanitized equipment did not ensure its sterilization. In contrast, 'active-closed' VHP exposure, in which IVC racks were assembled, sealed, and connected to AHU set to the VHP cycle, increased the proportion of chemical indicators that detected sterilizing levels of VHP inside the assembled equipment, and significantly decreased PCR-detectable S. xylosus inside the equipment. Supplementing bulk steam sterilization of the primary enclosure with VHP sterilization of the secondary housing equipment during room change-outs may help to mitigate opportunistic agents that jeopardize studies involving immunodeficient strains.

  9. Foundations of topological racks and quandles

    OpenAIRE

    Mohamed Moutuou, El-Kaioum; Elhamdadi, Mohamed

    2016-01-01

    We give a foundational account on topological racks and quandles. Specifically, we define the notions of ideals, kernels, units, and inner automorphism group in the context of topological racks. Further, we investigate topological rack modules and principal rack bundles. Central extensions of topological racks are then introduced providing a first step towards a general continuous cohomology theory for topological racks and quandles

  10. Active Vibration Isolation Devices with Inertial Servo Actuators

    Science.gov (United States)

    Melik-Shakhnazarov, V. A.; Strelov, V. I.; Sofiyanchuk, D. V.; Tregubenko, A. A.

    2018-03-01

    The use of active vibration isolation devices (AVIDs) in aerospace engineering is subject to the following restrictions. First, the volume for installing additional devices is always limited in instrument racks and compartments. Secondly, in many cases, it is impossible to add supports for servo actuators for fundamental or design considerations. In the paper, it has been shown that this problem can be solved if the inertial servo actuators are used in AVIDs instead of reference actuators. A transfer function has been theoretically calculated for an AVID controlled by inertial actuators. It has been shown that the volume of a six-mode single-housing AVID with inertial actuators can be 2-2.5 times smaller than that of devices with support actuators.

  11. Fuel storage rack

    International Nuclear Information System (INIS)

    Mollon, L.

    1977-01-01

    Disclosed is a storage rack for spent nuclear fuel elements comprising a multiplicity of elongated hollow containers of uniform cross-section, preferably square,some of said containers having laterally extending continuous flanges extending between adjacent containers and defining continuous elongated chambers therebetween for the reception of neutron absorbing panels. 18 claims, 7 figures

  12. Microgravity Disturbance Predictions in the Combustion Integrated Rack

    Science.gov (United States)

    Just, M.; Grodsinsky, Carlos M.

    2002-01-01

    This paper will focus on the approach used to characterize microgravity disturbances in the Combustion Integrated Rack (CIR), currently scheduled for launch to the International Space Station (ISS) in 2005. Microgravity experiments contained within the CIR are extremely sensitive to vibratory and transient disturbances originating on-board and off-board the rack. Therefore, several techniques are implemented to isolate the critical science locations from external vibration. A combined testing and analysis approach is utilized to predict the resulting microgravity levels at the critical science location. The major topics to be addressed are: 1) CIR Vibration Isolation Approaches, 2) Disturbance Sources and Characterization, 3) Microgravity Predictive Modeling, 4) Science Microgravity Requirements, 6) Microgravity Control, and 7) On-Orbit Disturbance Measurement. The CIR is using the Passive Rack Isolation System (PaRIS) to isolate the rack from offboard rack disturbances. By utilizing this system, CIR is connected to the U.S. Lab module structure by either 13 or 14 umbilical lines and 8 spring / damper isolators. Some on-board CIR disturbers are locally isolated by grommets or wire ropes. CIR's environmental and science on board support equipment such as air circulation fans, pumps, water flow, air flow, solenoid valves, and computer hard drives cause disturbances within the rack. These disturbers along with the rack structure must be characterized to predict whether the on-orbit vibration levels during experimentation exceed the specified science microgravity vibration level requirements. Both vibratory and transient disturbance conditions are addressed. Disturbance levels/analytical inputs are obtained for each individual disturber in a "free floating" condition in the Glenn Research Center (GRC) Microgravity Emissions Lab (MEL). Flight spare hardware is tested on an Orbital Replacement Unit (ORU) basis. Based on test and analysis, maximum disturbance level

  13. RACK1, A Multifaceted Scaffolding Protein: Structure and Function

    LENUS (Irish Health Repository)

    Adams, David R

    2011-10-06

    Abstract The Receptor for Activated C Kinase 1 (RACK1) is a member of the tryptophan-aspartate repeat (WD-repeat) family of proteins and shares significant homology to the β subunit of G-proteins (Gβ). RACK1 adopts a seven-bladed β-propeller structure which facilitates protein binding. RACK1 has a significant role to play in shuttling proteins around the cell, anchoring proteins at particular locations and in stabilising protein activity. It interacts with the ribosomal machinery, with several cell surface receptors and with proteins in the nucleus. As a result, RACK1 is a key mediator of various pathways and contributes to numerous aspects of cellular function. Here, we discuss RACK1 gene and structure and its role in specific signaling pathways, and address how posttranslational modifications facilitate subcellular location and translocation of RACK1. This review condenses several recent studies suggesting a role for RACK1 in physiological processes such as development, cell migration, central nervous system (CN) function and circadian rhythm as well as reviewing the role of RACK1 in disease.

  14. The ISS Fluids Integrated Rack (FIR): a Summary of Capabilities

    Science.gov (United States)

    Gati, F.; Hill, M. E.

    2002-01-01

    the top and bottom of the rack. Transmission of micro-gravity disturbances to and from the rack is minimized through the Active Rack Isolation System (ARIS). The environmental subsystem will utilize air and water to remove heat generated by facility and experimental hardware. The air will be circulated throughout the rack and will be cooled by an air-water heat exchanger. Water will be used directly to cool some of the FIR components and will also be available to cool experiment hardware as required. The electrical subsystem includes the Electrical Power Control Unit (EPCU), which provides 28 VDC and 120 VDC power to the facility and the experiment hardware. The EPCU will also provide power management and control functions, as well as fault protection capabilities. The FIR will provide access to the ISS gaseous nitrogen and vacuum systems. These systems are available to support experiment operations such as the purging of experimental cells, creating flows within experimental cells and providing dry conditions where needed. The FIR Command and Data Management subsystem (CDMS) provides command and data handling for both facility and experiment hardware. The Input Output Processor (IOP) provides the overall command and data management functions for the rack including downlinking or writing data to removable drives. The IOP will also monitor the health and status of the rack subsystems. The Image Processing and Storage Units (IPSU) will perform diagnostic control and image data acquisition functions. An IPSU will be able to control a digital camera, receive image data from that camera and process/ compress image data as necessary. The Fluids Science and Avionics Package (FSAP) will provide the primary control over an experiment. The FSAP contains various computer boards/cards that will perform data and control functions. To support the imaging needs, cameras and illumination sources will be available to the investigator. Both color analog and black and white digital

  15. Safety assessment of discharge chute isolation barrier preparation and installation activities. Revision 3

    International Nuclear Information System (INIS)

    Meichle, R.H.

    1994-01-01

    This revision adds a section addressing impacts of dropping surfacing tool and rack cutter on the basin floor, and corrects typographical errors. The safety assessment is made for the activities for the preparation and installation of the discharge chute isolation barriers. The safety assessment includes a hazard assessment and comparisons of potential accidents/events to those addressed by the current safety basis documentation. No significant hazards were identified. An evaluation against the USQ evaluation questions was made and the determination made that the activities do not represent a USQ. Hazard categorization techniques were used to provide a basis for readiness review classifications

  16. Specific induced activity profile at the rotary specimen rack of IPR-R1 TRIGA reactor after the introduction of a new pneumatic transfer tube

    International Nuclear Information System (INIS)

    Souza, Luiz Claudio Andrade; Zangirolami, Dante Marco; Maretti Junior, Fausto; Ferreira, Andrea Vidal

    2011-01-01

    The IPR-R1 TRIGA nuclear reactor is located in Belo Horizonte, Brazil, at the Nuclear Technology Development Center (Centro de Desenvolvimento da Tecnologia Nuclear, CDTN) of the National Committee on Nuclear Energy (Comissao Nacional de Energia Nuclear, CNEN). One of its irradiation devices is the rotary specimen rack (RSR), outside the reactor core, with forty irradiation positions arranged in a cylindrical geometry. In a previous work, the neutron fluence rate distribution at the RSR and its variation under different irradiation conditions were evaluated by means of specific induced activity measurements in samples of Al-0.1%Au reference material. Since then the core's configuration has been altered with the (re)introduction of another irradiation device, the pneumatic transfer tube 1 (PT-1). This paper aims at identifying and quantifying any changes in neutron fluence that such modification may have caused. (author)

  17. O-GlcNAcylation of RACK1 promotes hepatocellular carcinogenesis.

    Science.gov (United States)

    Duan, Fangfang; Wu, Hao; Jia, Dongwei; Wu, Weicheng; Ren, Shifang; Wang, Lan; Song, Shushu; Guo, Xinying; Liu, Fenglin; Ruan, Yuanyuan; Gu, Jianxin

    2018-06-01

    Aberrant oncogenic mRNA translation and protein O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) are general features during tumorigenesis. Nevertheless, whether and how these two pathways are interlinked remain unknown. Our previous study indicated that ribosomal receptor for activated C-kinase 1 (RACK1) promoted chemoresistance and growth in hepatocellular carcinoma (HCC). The aim of this study is to examine the role of RACK1 O-GlcNAcylation in oncogene translation and HCC carcinogenesis. The site(s) of RACK1 for O-GlcNAcylation was mapped by mass spectrometry analysis. HCC cell lines were employed to examine the effects of RACK1 O-GlcNAcylation on the translation of oncogenic factors and behaviors of tumor cells in vitro. Transgenic knock-in mice were used to detect the role of RACK1 O-GlcNAcylation in modulating HCC tumorigenesis in vivo. The correlation of RACK1 O-GlcNAcylation with tumor progression and relapse were analyzed in clinical HCC samples. We found that ribosomal RACK1 was highly modified by O-GlcNAc at Ser122. O-GlcNAcylation of RACK1 enhanced its protein stability, ribosome binding and interaction with PKCβII (PRKCB), leading to increased eukaryotic translation initiation factor 4E phosphorylation and translation of potent oncogenes in HCC cells. Genetic ablation of RACK1 O-GlcNAcylation at Ser122 dramatically suppressed tumorigenesis, angiogenesis, and metastasis in vitro and in diethylnitrosamine (DEN)-induced HCC mouse model. Increased RACK1 O-GlcNAcylation was also observed in HCC patient samples and correlated with tumor development and recurrence after chemotherapy. These findings demonstrate that RACK1 acts as key mediator linking O-GlcNAc metabolism to cap-dependent translation during HCC tumorigenesis. Targeting RACK1 O-GlcNAcylation provides promising options for HCC treatment. O-GlcNAcylation of ribosomal receptor for activated C-kinase 1 at the amino acid serine122 promotes its stability, ribosome localization and interaction

  18. ISS Material Science Research Rack HWIL Interface Simulation

    Science.gov (United States)

    Williams, Philip J.; Ballard, Gary H.; Crumbley, Robert T. (Technical Monitor)

    2002-01-01

    In this paper, the first Material Science Research Rack (MSRR-1) hardware-in-the-loop (HWIL) interface simulation is described. Dynamic Concepts developed this HWIL simulation system with funding and management provided by the Flight Software group (ED14) of NASA-MSFC's Avionics Department. The HWIL system has been used both as a flight software development environment and as a software qualification tool. To fulfill these roles, the HWIL simulator accurately models the system dynamics of many MSRR-1 subsystems and emulates most of the internal interface signals. The modeled subsystems include the Experiment Modules, the Thermal Environment Control System, the Vacuum Access System, the Solid State Power Controller Module, and the Active Rack Isolation Systems. The emulated signals reside on three separate MIL-STD-1553B digital communication buses, the ISS Medium Rate Data Link, and several analog controller and sensor signals. To enhance the range of testing, it was necessary to simulate several off-nominal conditions that may occur in the interfacing subsystems.

  19. High density fuel storage rack

    International Nuclear Information System (INIS)

    Zezza, L.J.

    1980-01-01

    High storage density for spent nuclear fuel assemblies in a pool achieved by positioning fuel storage cells of high thermal neutron absorption materials in an upright configuration in a rack. The rack holds the cells at required pitch. Each cell carries an internal fuel assembly support, and most cells are vertically movable in the rack so that they rest on the pool bottom. Pool water circulation through the cells and around the fuel assemblies is permitted by circulation openings at the top and bottom of the cells above and below the fuel assemblies

  20. Modular nuclear fuel assembly rack

    International Nuclear Information System (INIS)

    Davis, C.J.

    1982-01-01

    A modular nuclear fuel assembly rack constructed of an array of identical cells, each cell constructed of a plurality of identical flanged plates. The unique assembly of the plates into a rigid rack provides a cellular compartment for nuclear fuel assemblies and a cavity between the cells for accepting neutron absorbing materials thus allowing a closely spaced array. The modular rack size can be easily adapted to conform with available storage space. U-shaped flanges at the edges of the plates are nested together at the intersection of four cells in the array. A bar is placed at the intersection to lock the cells together

  1. RACK1-mediated translation control promotes liver fibrogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Min; Peng, Peike; Wang, Jiajun [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China); Wang, Lan; Duan, Fangfang [Institute of Biomedical Science, Fudan University, Shanghai 200032 (China); Jia, Dongwei, E-mail: jiadongwei@fudan.edu.cn [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China); Ruan, Yuanyuan, E-mail: yuanyuanruan@fudan.edu.cn [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China); Gu, Jianxin [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China); Institute of Biomedical Science, Fudan University, Shanghai 200032 (China)

    2015-07-31

    Activation of quiescent hepatic stellate cells (HSCs) is the central event of liver fibrosis. The translational machinery is an optimized molecular network that affects cellular homoeostasis and diseases, whereas the role of protein translation in HSCs activation and liver fibrosis is little defined. Our previous report suggests that up-regulation of receptor for activated C-kinase 1(RACK1) in HSCs is critical for liver fibrogenesis. In this study, we found that RACK1 promoted macrophage conditioned medium (MCM)-induced assembly of eIF4F and phosphorylation of eIF4E in primary HSCs. RACK1 enhanced the translation and expression of pro-fibrogenic factors collagen 1α1, snail and cyclin E1 induced by MCM. Administration of PP242 or knock-down of eIF4E suppressed RACK1-stimulated collagen 1α1 production, proliferation and migration in primary HSCs. In addition, depletion of eIF4E attenuated thioacetamide (TAA)-induced liver fibrosis in vivo. Our data suggest that RACK1-mediated stimulation of cap-dependent translation plays crucial roles in HSCs activation and liver fibrogenesis, and targeting translation initiation could be a promising strategy for the treatment of liver fibrosis. - Highlights: • RACK1 induces the assembly of eIF4F and phosphorylation of eIF4E in primary HSCs. • RACK1 stimulates the translation of collagen 1α1, snail and cyclin E1 in HSCs. • RACK1 promotes HSCs activation via cap-mediated translation. • Depletion of eIF4E suppresses liver fibrogenesis in vivo.

  2. RACK1-mediated translation control promotes liver fibrogenesis

    International Nuclear Information System (INIS)

    Liu, Min; Peng, Peike; Wang, Jiajun; Wang, Lan; Duan, Fangfang; Jia, Dongwei; Ruan, Yuanyuan; Gu, Jianxin

    2015-01-01

    Activation of quiescent hepatic stellate cells (HSCs) is the central event of liver fibrosis. The translational machinery is an optimized molecular network that affects cellular homoeostasis and diseases, whereas the role of protein translation in HSCs activation and liver fibrosis is little defined. Our previous report suggests that up-regulation of receptor for activated C-kinase 1(RACK1) in HSCs is critical for liver fibrogenesis. In this study, we found that RACK1 promoted macrophage conditioned medium (MCM)-induced assembly of eIF4F and phosphorylation of eIF4E in primary HSCs. RACK1 enhanced the translation and expression of pro-fibrogenic factors collagen 1α1, snail and cyclin E1 induced by MCM. Administration of PP242 or knock-down of eIF4E suppressed RACK1-stimulated collagen 1α1 production, proliferation and migration in primary HSCs. In addition, depletion of eIF4E attenuated thioacetamide (TAA)-induced liver fibrosis in vivo. Our data suggest that RACK1-mediated stimulation of cap-dependent translation plays crucial roles in HSCs activation and liver fibrogenesis, and targeting translation initiation could be a promising strategy for the treatment of liver fibrosis. - Highlights: • RACK1 induces the assembly of eIF4F and phosphorylation of eIF4E in primary HSCs. • RACK1 stimulates the translation of collagen 1α1, snail and cyclin E1 in HSCs. • RACK1 promotes HSCs activation via cap-mediated translation. • Depletion of eIF4E suppresses liver fibrogenesis in vivo

  3. 21 CFR 886.1750 - Skiascopic rack.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Skiascopic rack. 886.1750 Section 886.1750 Food... DEVICES OPHTHALMIC DEVICES Diagnostic Devices § 886.1750 Skiascopic rack. (a) Identification. A skiascopic rack is a device that is a rack and a set of attached ophthalmic lenses of various dioptric strengths...

  4. Link invariants from finite Coxeter racks

    OpenAIRE

    Nelson, Sam; Wieghard, Ryan

    2008-01-01

    We study Coxeter racks over $\\mathbb{Z}_n$ and the knot and link invariants they define. We exploit the module structure of these racks to enhance the rack counting invariants and give examples showing that these enhanced invariants are stronger than the unenhanced rack counting invariants.

  5. Storage rack for spent nuclear fuels

    International Nuclear Information System (INIS)

    Kiyama, Yoichi.

    1996-01-01

    A storage rack comprises a number of rack cells for containing spent nuclear fuels and two upper and lower rack support plates. Small through holes are formed to lateral walls of the rack cell each at a position slightly above the position of the upper rack support plate. Finger members each having a protrusion which fits the small through hole is secured at the upper surface of the upper rack support plate. The finger member is a metal leaf-spring erected at the periphery of a rack insertion hole of the rack support plate. Gaps for allowing thermal expansion of the rack cell are formed each between the edge of the rack cell insertion hole of the rack support plate and the rack cell, and between the lower edge of the small through hole on a side wall of the rack cell and the lower portion of the protrusion of the finger member. If the rack cell is inserted to a bottom, the protrusion of the finger member fits the small through hole on the side of the rack cell. With such a constitution, the rack cell is prevented from withdrawing in conjunction with removal of fuels. (I.N.)

  6. Working safely with electronics racks

    CERN Document Server

    Simon Baird, HSE Unit Head

    2016-01-01

    Think of CERN and you’ll probably think of particle accelerators and detectors. These are the tools of the trade in particle physics, but behind them are the racks of electronics that include power supplies, control systems and data acquisition networks.   Inside an electronics rack: danger could be lurking if the rack is not powered off. In routine operation, these are no more harmful than the home entertainment system in your living room. But unscrew the cover and it’s a different matter. Even after following appropriate training, and with formal authorisation from your group leader or equivalent to carry out electrical work or any work in the vicinity of electrical hazards, and even with extensive experience of carrying out such operations, it’s important to incorporate safe working practices into your routine. At CERN, before the racks of electronics reach their operational configurations for the accelerators and detectors, they play a vital role in test set-ups ...

  7. AP Alternatives Modular Solar Racking

    OpenAIRE

    ECT Team, Purdue

    2015-01-01

    Solar panels have been used to generate and supply electricity in various applications such as residential and commercial buildings. Therefore, a need for a quicker approach to installation as well as the need to reduce workmanship to speed up the process is needed. Solar racks are developed through the use of automated equipment to “preassemble the racking hardware, pre-install the solar modules, and drive helical anchors into the ground rapidly”. So instead of installing one panel at a time...

  8. HOTLink rack monitor

    International Nuclear Information System (INIS)

    Al R Franck et al.

    2001-01-01

    A remote data acquisition chassis, called a HOTLink Rack Monitor, HRM, has been developed for use in the Fermilab control system. This chassis provides for 64 analog input channels, 8 analog output channels, and 8 bytes of digital I/O. The interface to the host VMEbus crate is by way of a 320 MHz HOTLink serial connection to a PMC mezzanine module. With no processor intervention, all data sources in the remote chassis are read at 100 sec intervals, time stamped, and stored in a 2 MB circular buffer on the PMC module. In operation, the memory always contains the most recent 16 k samples of 10 kHz data from all 64 analog input channels. An expansion module that resides in the HRM chassis records snapshot data for 8 analog channels, each channel consisting of up to 16 k readings, digitized at rates up to 10 MHz. Snapshot data is also returned and stored in buffers on the PMC module. Because the HRM presents a memory-mapped interface to the host, it is independent of the operating system and may be used in any system that supports PMC mezzanine modules

  9. Active Fault Isolation in MIMO Systems

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik; Poulsen, Niels Kjølstad

    2014-01-01

    isolation is based directly on the input/output s ignals applied for the fault detection. It is guaranteed that the fault group includes the fault that had occurred in the system. The second step is individual fault isolation in the fault group . Both types of isolation are obtained by applying dedicated......Active fault isolation of parametric faults in closed-loop MIMO system s are considered in this paper. The fault isolation consists of two steps. T he first step is group- wise fault isolation. Here, a group of faults is isolated from other pos sible faults in the system. The group-wise fault...

  10. Seismic design method of free standing rack

    International Nuclear Information System (INIS)

    Taniguchi, Katsuhiko; Okuno, Daisaku; Iwasaki, Akihisa; Nekomoto, Yoshitsugu; Matsuoka, Toshihiro

    2013-01-01

    For high earthquake resistance and ease of installation, free standing racks which are not anchored to the pool floor or walls has been adopted in many countries. Under the earthquake, the response of the free standing rack is highly nonlinear and involves a complex combination of motions (sliding, rocking, twisting, and turning) and impacts between the fuel assemblies and the fuel cell walls, rack-to-rack, and the pit floor and rack pedestals. We carried out seismic experiments on the full-scale rack model in water and dry conditions to obtain the fundamental data about free standing rack (sliding, rocking and turning motions). We have developed the nonlinear dynamic analysis method to predict seismic response for the free standing rack utilizing the full-scale test result and verified the analysis evaluation method of the rack by comparison of test result. (author)

  11. RACK(1) to the future - a historical perspective

    LENUS (Irish Health Repository)

    Ron, Dorit

    2013-08-01

    This perspective summarises the first and long overdue RACK1 meeting held at the University of Limerick, Ireland, May 2013, in which RACK1’s role in the immune system, the heart and the brain were discussed and its contribution to disease states such as cancer, cardiac hypertrophy and addiction were described. RACK1 is a scaffolding protein and a member of the WD repeat family of proteins. These proteins have a unique architectural assembly that facilitates protein anchoring and the stabilisation of protein activity. A large body of evidence is accumulating which is helping to define the versatile role of RACK1 in assembling and dismantling complex signaling pathways from the cell membrane to the nucleus in health and disease. In this commentary, we first provide a historical perspective on RACK1. We also address many of the pertinent and topical questions about this protein such as its role in transcription, epigenetics and translation, its cytoskeletal contribution and the merits of targeting RACK1 in disease.

  12. Storage racks for spent fuels

    International Nuclear Information System (INIS)

    Inoue, Tatsumi.

    1983-01-01

    Purpose: To provide a storage rack which is light in weight, excellent in constructing performance, earthquake proof and allowable for thermal displacement. Constitution: The rack frame is constituted by disposing mounting seat plates at the legs of profiled-steel post members on a rack-mounting plate as a mounting seat by way of mounting liner plates and then weld-joining each of upper, medium and lower beam members and truss members of the profiled-steel posts by the use of connecting plates. Guide plates are laid transversely and joined onto each of the thus formed upper, medium and lower stage frames. Since apertures for mounting guide tubes are opened to each of the guide plates at a predetermined pitch, the storage rack unit is constituted by mounting guide tubes to the apertures. Then, the storage rack is mounted by means of slide keys to the mounting plates where slight amount of sliding or thermal displacement can be absorbed. (Horiuchi, T.)

  13. Storage rack for fuel cell receiving shrouds

    International Nuclear Information System (INIS)

    Mollon, L.

    1978-01-01

    Disclosed is a rack for receiving a multiplicity of vertical tubular shrouds or tubes for storing spent nuclear fuel cells. The rack comprises a plurality of horizontally reticulated frames interconnected by tension rods and spacing tubes surrounding the rods

  14. Rack Protection Monitor - A Simple System

    International Nuclear Information System (INIS)

    Orr, S.

    1997-12-01

    The Rack Protection Monitor is a simple, fail-safe device to monitor smoke, temperature and ventilation sensors. It accepts inputs from redundant sensors and has a hardwired algorithm to prevent nuisance power trips due to random sensor failures. When a sensor is triggered the Rack Protection Monitor latches and annunicates the alarm. If another sensor is triggered, the Rack Protection Monitor locally shuts down the power to the relay rack and sends alarm to central control

  15. 40 CFR 63.1105 - Transfer racks.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 10 2010-07-01 2010-07-01 false Transfer racks. 63.1105 Section 63... Technology Standards § 63.1105 Transfer racks. (a) Design requirements. The owner or operator shall equip each transfer rack with one of the control options listed in paragraphs (a)(1) through (4) of this...

  16. Compact fuel storage rack for fuel pools

    International Nuclear Information System (INIS)

    Parras, F.; Louvat, J.P.

    1986-01-01

    ETS LEMER and FRAMATOME propose a new compact storage rack. This rack permits a considerable increase of the storage capacity of cooling pools. A short description of the structure and the components is presented, to propose racks that are: . Inalterable, . Compact, . Insensitive to earthquakes. Installation in pools already in operation is simplified by their light structure and the bearing device [fr

  17. 21 CFR 882.4215 - Clip rack.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Clip rack. 882.4215 Section 882.4215 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Surgical Devices § 882.4215 Clip rack. (a) Identification. A clip rack is a...

  18. ATLAS DAQ/HLT rack DCS

    International Nuclear Information System (INIS)

    Ermoline, Yuri; Burckhart, Helfried; Francis, David; Wickens, Frederick J.

    2007-01-01

    The ATLAS Detector Control System (DCS) group provides a set of standard tools, used by subsystems to implement their local control systems. The ATLAS Data Acquisition and High Level Trigger (DAQ/HLT) rack DCS provides monitoring of the environmental parameters (air temperatures, humidity, etc.). The DAQ/HLT racks are located in the underground counting room (20 racks) and in the surface building (100 racks). The rack DCS is based on standard ATLAS tools and integrated into overall operation of the experiment. The implementation is based on the commercial control package and additional components, developed by CERN Joint Controls Project Framework. The prototype implementation and measurements are presented

  19. Isolated polypeptide having arabinofuranosidase activity

    Science.gov (United States)

    Foreman, Pamela; Van Solingen, Pieter; Goedegebuur, Frits; Ward, Michael

    2010-02-23

    Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry. TABLE-US-00001 cip1 cDNA sequence (SEQ ID NO: 1) GACTAGTTCA TAATACAGTA GTTGAGTTCA TAGCAACTTC 50 ACTCTCTAGC TGAACAAATT ATCTGCGCAA ACATGGTTCG CCGGACTGCT 100 CTGCTGGCCC TTGGGGCTCT CTCAACGCTC TCTATGGCCC AAATCTCAGA 150 CGACTTCGAG TCGGGCTGGG ATCAGACTAA ATGGCCCATT TCGGCACCAG 200 ACTGTAACCA GGGCGGCACC GTCAGCCTCG ACACCACAGT AGCCCACAGC 250 GGCAGCAACT CCATGAAGGT CGTTGGTGGC CCCAATGGCT ACTGTGGACA 300 CATCTTCTTC GGCACTACCC AGGTGCCAAC TGGGGATGTA TATGTCAGAG 350 CTTGGATTCG GCTTCAGACT GCTCTCGGCA GCAACCACGT CACATTCATC 400 ATCATGCCAG ACACCGCTCA GGGAGGGAAG CACCTCCGAA TTGGTGGCCA 450 AAGCCAAGTT CTCGACTACA ACCGCGAGTC CGACGATGCC ACTCTTCCGG 500 ACCTGTCTCC CAACGGCATT GCCTCCACCG TCACTCTGCC TACCGGCGCG 550 TTCCAGTGCT TCGAGTACCA CCTGGGCACT GACGGAACCA TCGAGACGTG 600 GCTCAACGGC AGCCTCATCC CGGGCATGAC CGTGGGCCCT GGCGTCGACA 650 ATCCAAACGA CGCTGGCTGG ACGAGGGCCA GCTATATTCC GGAGATCACC 700 GGTGTCAACT TTGGCTGGGA GGCCTACAGC GGAGACGTCA ACACCGTCTG 750 GTTCGACGAC ATCTCGATTG CGTCGACCCG CGTGGGATGC GGCCCCGGCA 800 GCCCCGGCGG TCCTGGAAGC TCGACGACTG GGCGTAGCAG CACCTCGGGC 850 CCGACGAGCA CTTCGAGGCC AAGCACCACC ATTCCGCCAC CGACTTCCAG 900 GACAACGACC GCCACGGGTC CGACTCAGAC ACACTATGGC CAGTGCGGAG 1000 GGATTGGTTA CAGCGGGCCT ACGGTCTGCG CGAGCGGCAC GACCTGCCAG 1050 GTCCTGAACC CATACTACTC CCAGTGCTTA TAAGGGGATG AGCATGGAGT 1100 GAAGTGAAGT GAAGTGGAGA GAGTTGAAGT GGCATTGCGC TCGGCTGGGT 1150 AGATAAAAGT CAGCAGCTAT GAATACTCTA TGTGATGCTC ATTGGCGTGT 1200 ACGTTTTAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 1250 AAAAAAAAAA AAAAAAAAAG GGGGCGGCCG C 1271

  20. Hydrodynamics induced vibration to trash-racks

    International Nuclear Information System (INIS)

    Sadrnejad, A.

    2002-01-01

    In conventional power plants trash-racks are provided at the intakes to protect the turbines. In pumped storage plants, the draft tube or tailrace must also have trash-racks to protect the units while pumping. Because the loads believed to cause many failures of trash-racks are dynamic in nature, it is important to understand the dynamic characteristics of trash-racks structures in general and a single rack in particular. The classical added-mass solution structure-fluid dynamic interaction is known as an approximate solution procedure. An accurate added-mass approach mixed with implementation in finite element framework is proposed. In this proposal, experimental conclusions, supported by theory, led to presentation of more accurate results in vibration of trash-racks. This numerical solution as a powerful method to solve such a complex problem can be employed to carry out dynamic characteristics of these structures while vibrating in water

  1. Server rack for improved data center management

    Science.gov (United States)

    Bermudez Rodriguez, Sergio A.; Hamann, Hendrik F.; Wehle, Hans-Dieter

    2018-01-09

    Methods and systems for data center management include collecting sensor data from one or more sensors in a rack; determining a location and identifying information for each asset in the rack using a set of asset tags associated with respective assets; communicating the sensor and asset location to a communication module; receiving an instruction from the communication module; and executing the received instruction to change a property of the rack.

  2. Framatome offers new high density Cadminox racks

    International Nuclear Information System (INIS)

    Anon.

    1985-01-01

    Framatome have developed a new material called Cadminox for use in high density spent fuel storage racks. It is claimed that Cadminox will remain stable stable in pond storage when racks submerged in boronated water are irradiated by the spent fuel they contain. A brief description of the storage module is given, including the aseismic bearing device which minimises loads on pond walls, racks and fuel assemblies. (UK)

  3. Noncontact rack-pinion-rack device as a differential vibration sensor.

    Science.gov (United States)

    Miri, MirFaez; Nasiri, Mojtaba

    2010-07-01

    We study a nanoscale system composed of one corrugated cylinder (pinion) placed between two corrugated plates (racks). The pinion and racks have no mechanical contact, but are coupled via the lateral Casimir force-one of the most spectacular consequences of quantum fluctuations of the electromagnetic field. The noncontact design of the device could help with the noteworthy wear problem in nanoscale mechanical systems. We consider the case where both racks undergo harmonic lateral motion. We assume that the amplitude, frequency, and phase of one of the racks are known. We show that probing the pinion motion, one can determine the vibration characteristics of the other rack.

  4. Knot invariants derived from quandles and racks

    OpenAIRE

    Kamada, Seiichi

    2002-01-01

    The homology and cohomology of quandles and racks are used in knot theory: given a finite quandle and a cocycle, we can construct a knot invariant. This is a quick introductory survey to the invariants of knots derived from quandles and racks.

  5. High density aseismic spent fuel storage racks

    International Nuclear Information System (INIS)

    Louvat, J.P.

    1985-05-01

    After the reasons of the development of high density aseismic spent fuel racks by FRAMATOME and LEMER, a description is presented, as also the codes, standards and regulations used to design this FRAMATOME storage rack. Tests have been carried out concerning criticality, irradiation of Cadminox, corrosion of the cell, and the seismic behaviour

  6. Rack for nuclear fuel elements

    International Nuclear Information System (INIS)

    Rubinstein, H.J.; Gordon, C.B.; Robison, A.; Clark, P.M.

    1977-01-01

    Disclosed is a rack for storing spent nuclear fuel elements in which a plurality of aligned rows of upright enclosures of generally square cross-sectional areas contain vertically disposed spent fuel elements. Each fuel element is supported at the lower end thereof by a respective support that rests on the floor of the spent fuel pool for a nuclear power plant. An open rack frame is employed as an upright support for the enclosures containing the spent fuel elements. Legs at the lower corners of the frame rest on the floor of the pool to support the frame. In one exemplary embodiment, the support for the fuel element is in the form of a base on which a fuel element rests and the base is supported by legs. In another exemplary embodiment, each fuel element is supported on the pool floor by a self-adjusting support in the form of a base on which a fuel element rests and the base rests on a ball or swivel joint for self-alignment. The lower four corners of the frame are supported by legs adjustable in height for leveling the frame. Each adjustable frame leg is in the form of a base resting on the pool floor and the base supports a threaded post. The threaded post adjustably engages a threaded column on which rests the lower end of the frame. 16 claims, 14 figures

  7. Refurbishment of the rotating rack of the OSU TRIGA MKII reactor

    International Nuclear Information System (INIS)

    Higginbotham, J.F.; Dodd, B.; Pratt, D.S.; Anderson, T.V.

    1992-01-01

    Many TRIGA reactors have experienced operational difficulties with the rotating racks used for sample irradiation. Generally the rack gradually becomes more difficult to rotate until it finally seizes. The recommended action at that point is replacement of the entire facility at a significant cost. The purpose of this paper is to describe the symptoms leading to rack failure and to present the results of a refurbishment procedure that does not involve the use of solvents which create mixed chemical and radioactive hazardous waste. The primary reason for rack failure is the buildup of sludge produced through irradiation of lubrication oil. The refurbishment procedure involves using a commercially available degreasing solution which can be pumped into and out of the rack with the objective of removing this sludge. The solution used is sold under the trade name 'Simple Green'. No radioactive material was detected on smear or air samples taken of the work area during the reifurbishment activities and the rack rotates freely in both direction even after eighteen months of operation. The only disadvantage to performing this procedure has been the need to maintain a very aggressive contamination control program when unloading samples from the rack. A very fine particulate material attaches to the outside of tubes used to encapsulate samples. This material can produce contamination levels of 10,000 dpm/100 cm 2 in the worst cases but will typically produce local hot spots on the order of 1000 dpm. (author)

  8. Antioxidant activity directed isolations form punica granatum

    International Nuclear Information System (INIS)

    Siddiqi, R.; Saeed, M.G.; Sayeed, S.A.

    2012-01-01

    The extracts derived from pomegranate juice following antioxidant activity directed isolation were screened for their antioxidant activity through their ability to scavenge 2,2- diphenyl-l-picrylhydrazyl (DPPH) radicals. Only fractions which exhibited >50 / 0 DPPH scavenging effect at each step of isolation were selected for further purification and their ability to reduce peroxide formation (peroxide value) in heated com oil. Phytochemical analysis of the pure compounds finally obtained, revealed the presence of pelargonidin-3- galactose (Pg-3-galactose), cyanidin-3-glucose (Cy-3-Glucose), gallic acid, quercetin and myricetin in the fractions exhibiting >50% DPPH scavenging potential. The order of antioxidant activity of these pure compounds by DPPH method was found to be gallic acid> quercetin> myricetin> Cy-3-galactose> Pg-3-Glucose while order with respect to reduction in peroxide value (PV) was the reverse of DPPH. (author)

  9. Enzymatic activity of fungi isolated from crops

    Directory of Open Access Journals (Sweden)

    Wioletta A. Żukiewicz-Sobczak

    2016-12-01

    Full Text Available Aim: To detect and assess the activity of extracellular hydrolytic enzymes and to find differences in enzymograms between fungi isolated from wheat and rye samples and grown on Czapek-Dox Broth and Sabouraud Dextrose Broth enriched with cereal (wheat or rye. Isolated strains were also classified in the scale of biosafety levels (BSL. Material and methods: The study used 23 strains of fungi cultured from samples of wheat and rye (grain, grain dust obtained during threshing and soil collected in the Lublin region (eastern Poland. API ZYM test (bioMérieux was carried out according to the manufacturer’s instructions. Classification of BSL (Biosafety levels was based on the current literature. Results : High enzymatic activity was found in strains cultured in media containing 1% of wheat grain ( Bipolaris holmi, Penicillium decumbens and with an addition of 1% of rye grain ( Cladosporium herbarum, Aspergillus versicolor, Alternaria alternata . The total number of enzymes varied depending on the type of media, and in most cases it was higher in the culture where an addition of cereal grains was used. Conclusions : Isolated strains of fungi reveal differences in the profiles of the enzyme assay. It can be assumed that the substrate enriched in grains stimulate the higher activity of mold enzymes. Key words: enzymatic activity, mold fungi, zymogram, biohazards.

  10. Seismic analysis of freestanding fuel racks

    International Nuclear Information System (INIS)

    Gilmore, C.B.

    1982-01-01

    This paper presents a nonlinear transient dynamic time-history analysis of freestanding spent fuel storage racks subjected to seismic excitation. This type of storage rack is structurally unrestrained and submerged in water in the spent fuel pool of a nuclear power complex, holds (spent) fuel assemblies which have been removed from the reactor core. Nonlinearities in the fuel rack system include impact between the fuel assembly and surrounding cell due to clearances between them, friction due to sliding between the fuel rack support structure and spent fuel pool floor, and the lift-off of the fuel rack support structure from the spent fuel pool floor. The analysis of the fuel rack system includes impacting due to gap closures, energy losses due to impacting bodies, Coulomb damping between sliding surfaces, and hydrodynamic mass effects. Acceleration time history excitation development is discussed. Modeling considerations, such as the initial status of nonlinear elements, number of mode shapes to include in the analysis, modal damping, and integration time-step size are presented. The response of the fuel rack subjected to two-dimensional seismic excitation is analyzed by the modal superposition method, which has resulted in significant computer cost savings when compared to that of direct integration

  11. Isolation and biological activity of frankiamide.

    Science.gov (United States)

    Haansuu, J P; Klika, K D; Söderholm, P P; Ovcharenko, V V; Pihlaja, K; Haahtela, K K; Vuorela, P M

    2001-07-01

    An antibiotic produced by the symbiotic actinomycete Frankia strain AiPs1 was isolated from culture broth using optimized thin-layer chromatography and high-performance liquid chromatography (HPLC) methods. The novel compound that was isolated, dubbed frankiamide, displayed antimicrobial activity against all 14 Gram-positive bacterial strains and six pathogenic fungal strains tested. The pathogenic actinomycete Clavibacter michiganensis and the oomycete Phytophthora were especially susceptible. In addition to displaying antimicrobial activity, frankiamide also strongly inhibited 45Ca(2+) fluxes in clonal rat pituitary GH4C1 tumor cells and was comparable to a frequently used calcium antagonist, verapamil hydrochloride. The results of HPLC analysis, supported by both nuclear magnetic resonance and mass spectroscopy studies, showed that frankiamide has a high affinity for Na(+) ions.

  12. Active vibration isolation of high precision machines

    CERN Document Server

    Collette, C; Artoos, K; Hauviller, C

    2010-01-01

    This paper provides a review of active control strategies used to isolate high precisionmachines (e.g. telescopes, particle colliders, interferometers, lithography machines or atomic force microscopes) from external disturbances. The objective of this review is to provide tools to develop the best strategy for a given application. Firstly, the main strategies are presented and compared, using single degree of freedom models. Secondly, the case of huge structures constituted of a large number of elements, like particle colliders or segmented telescopes, is considered.

  13. From racks to pointed Hopf algebras

    OpenAIRE

    Andruskiewitsch, Nicolás; Graña, Matı́as

    2003-01-01

    A fundamental step in the classification of finite-dimensional complex pointed Hopf algebras is the determination of all finite-dimensional Nichols algebras of braided vector spaces arising from groups. The most important class of braided vector spaces arising from groups is the class of braided vector spaces (CX, c^q), where C is the field of complex numbers, X is a rack and q is a 2-cocycle on X with values in C^*. Racks and cohomology of racks appeared also in the work of topologists. This...

  14. 10 CFR 36.35 - Source rack protection.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Source rack protection. 36.35 Section 36.35 Energy NUCLEAR... Requirements for Irradiators § 36.35 Source rack protection. If the product to be irradiated moves on a product conveyor system, the source rack and the mechanism that moves the rack must be protected by a barrier or...

  15. 40 CFR 63.422 - Standards: Loading racks.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 9 2010-07-01 2010-07-01 false Standards: Loading racks. 63.422....422 Standards: Loading racks. (a) Each owner or operator of loading racks at a bulk gasoline terminal... facility” used in § 60.502 of this chapter means the loading racks that load gasoline cargo tanks at the...

  16. Practical research of free standing rack. Seismic experiment study on full scale free standing rack

    International Nuclear Information System (INIS)

    Iwasaki, Akihisa; Nekomoto, Yoshitsugu; Morita, Hideyuki; Taniguchi, Katsuhiko; Okuno, Daisaku; Matsuoka, Toshihiro; Chigusa, Naoki

    2015-01-01

    The spent fuel taken out of a plant reactor is temporarily stored in a spent fuel rack. This fuel will often have to be stored in the rack for long periods before it can be moved to a reprocessing facility. Therefore, the spent fuel rack must have a high tolerance against big seismic loads. The free standing spent fuel rack has been developed as the optimal equipment meeting these requirements. It can be placed on the spent fuel pool floor without fixation to any support structure. Response of the free standing rack is reduced by the effect of the water and friction force on the spent fuel pool floor. For nuclear plant safety, it is necessary to understand the free standing rack behavior under earthquake in pools to verify the design of free standing racks and peripheral components. Several tests on a shaking table have been conducted on full-scale one free standing rack in air and in water, and sliding and rocking have been measured. The rack response is very complex and the study necessitates to take into account the sliding, the rocking, the effect of the water and of the arrangement of the fuel assemblies inside. (author)

  17. Rack assembly for mounting solar modules

    Science.gov (United States)

    Plaisted, Joshua Reed; West, Brian

    2010-12-28

    A rack assembly is provided for mounting solar modules over an underlying body. The rack assembly may include a plurality of rail structures that are arrangeable over the underlying body to form an overall perimeter for the rack assembly. One or more retention structures may be provided with the plurality of rail structures, where each retention structure is configured to support one or more solar modules at a given height above the underlying body. At least some of the plurality of rail structures are adapted to enable individual rail structures o be sealed over the underlying body so as to constrain air flow underneath the solar modules. Additionally, at least one of (i) one or more of the rail structures, or (ii) the one or more retention structures are adjustable so as to adapt the rack assembly to accommodate solar modules of varying forms or dimensions.

  18. Experimental motion behavior of submerged fuel racks

    International Nuclear Information System (INIS)

    Ellingson, F.J.; Wachter, W.; Moscardini, R.L.

    1989-01-01

    The design of submerged nuclear storage racks for light water reactor nuclear fuel has undergone a change from fixed position to a free-standing arrangement. Seismic analysis of the motion of the free-standing racks requires three-dimensional computer modeling that uses past studies of hydrodynamic mass and hydraulic coupling for rigid flat plates. This paper describes the results of experiments that show a reduced value for hydrodynamic mass and coupling forces when flexible elements are involved. To support this work, experiments were run with two full-scale welded box sections submerged in a water tank. The preliminary results indicate reduction in hydrodynamic mass due to box wall flexibility, a lack of impacting of box wall to box wall over the entire frequency range, and large hydrodynamic coupling forces under all test conditions. It is hypothesized that the coupling forces are sufficiently strong to prevent rotational motion of one rack when surrounded by adjacent racks

  19. Numerical activities on seismic isolation in Italy

    International Nuclear Information System (INIS)

    Bettinali, F.; Martelli, A.; Bonacina, G.; Olivieri, M.

    1992-01-01

    The numerical activities which are in progress in Italy in the framework of the seismic isolation studies mainly concern the definition of models for bearings and isolated structures, and their use for test design and the analysis of experimental results. Simple bearing models have been set up, and the development of finite-element (f.e.) three-dimensional (3D) and 2D axisymmetric models is in progress. simple models have been based on the results of single bearing tests: models formed by a spring in parallel to a viscous damper, where both horizontal stiffness and viscous damping vary with displacements, have been developed by ENEA. Models based on hysteretic damping have also been developed by DISP and ISMES. Detailed bearing models include separate elements for the rubber and steel plates. A 3D model has been implemented by ENEA in the ABAQUS code. Linear elastic calculations have been performed with this model. The implementation of an elastic-plastic model for steel is also being completed, together with that of a hyper elastic model of the rubber, based on tests on specimens. Detailed models will be validated based on measured data. They will be used for bearing design and analysis of the effects of defects: some bearings with artificial defects have been fabricated to this purpose. As to the isolated structures, finite-difference programs were set up for the analysis of such structures in the case that they can be represented by sets of one-degree-of-freedom oscillators. The program ISOLA includes the aforementioned simple bearing model of ENEA, where both stiffness and damping depend on displacement and the effects of viscous creep are accounted for. A similar program has been based on the bearing model developed at ISMES. These models have been successfully used to analyse the experimental results concerning both isolated structure mock-ups and actual isolated buildings, based on the single bearing test data for both horizontal stiffness and damping (see a

  20. Fuel consumption impacts of auto roof racks

    International Nuclear Information System (INIS)

    Chen, Yuche; Meier, Alan

    2016-01-01

    The after-market roof rack is one of the most common components attached to a vehicle for carrying over-sized items, such as bicycles and skis. It is important to understand these racks’ fuel consumption impacts on both individual vehicles and the national fleet because they are widely used. We estimate the national fuel consumption impacts of roof racks using a bottom-up approach. Our model incorporates real-world data and vehicle stock information to enable assessing fuel consumption impacts for several categories of vehicles, rack configurations, and usage conditions. In addition, the model draws on two new data-gathering techniques, on-line forums and crowd-sourcing. The results show that nationwide, roof racks are responsible for 0.8‰ of light duty vehicle fuel consumption in 2015, corresponding to 100 million gallons of gasoline per year. Sensitivity analyses show that results are most sensitive to the fraction of vehicles with installed roof racks but carrying no equipment. The aerodynamic efficiency of typical roof racks can be greatly improved and reduce individual vehicle fuel consumption; however, government policies to minimize extensive driving with empty racks—if successful—could save more fuel nationally. - Highlights: •First estimate of national energy impacts of auto roof racks—about 1‰. •A bottom-up approach reveals details of the fuel consumption penalty caused by racks. •Two novel data collection techniques, on-line forums and crowd-sourcing, improve estimate. •Technical and behavioral policies could significantly cut fuel penalties from roof racks.

  1. ACRR fuel storage racks criticality safety analysis

    International Nuclear Information System (INIS)

    Bodette, D.E.; Naegeli, R.E.

    1997-10-01

    This document presents the criticality safety analysis for a new fuel storage rack to support modification of the Annular Core Research Reactor for production of molybdenum-99 at Sandia National Laboratories, Technical Area V facilities. Criticality calculations with the MCNP code investigated various contingencies for the criticality control parameters. Important contingencies included mix of fuel element types stored, water density due to air bubbles or water level for the over-moderated racks, interaction with existing fuel storage racks and fuel storage holsters in the fuel storage pool, neutron absorption of planned rack design and materials, and criticality changes due to manufacturing tolerances or damage. Some limitations or restrictions on use of the new fuel storage rack for storage operations were developed through the criticality analysis and are required to meet the double contingency requirements of criticality safety. As shown in the analysis, this system will remain subcritical under all credible upset conditions. Administrative controls are necessary for loading, moving, and handling the storage rack as well as for control of operations around it. 21 refs., 16 figs., 4 tabs

  2. First Materials Science Research Rack Capabilities and Design Features

    Science.gov (United States)

    Schaefer, D.; King, R.; Cobb, S.; Whitaker, Ann F. (Technical Monitor)

    2001-01-01

    The first Materials Science Research Rack (MSRR-1) will accommodate dual Experiment Modules (EM's) and provide simultaneous on-orbit processing operations capability. The first international Materials Science Experiment Module for the MSRR-1 is an international cooperative research activity between NASA's Marshall Space Flight Center (MSFC) and the European Space Agency's (ESA) European Space Research and Technology Center. (ESTEC). This International Standard Payload Rack (ISPR) will contain the Materials Science Laboratory (MSL) developed by ESA as an Experiment Module. The MSL Experiment Module will accommodate several on-orbit exchangeable experiment-specific Module Inserts. Module Inserts currently planned are a Quench Module Insert, Low Gradient Furnace, Solidification with Quench Furnace, and Diffusion Module Insert. The second Experiment Module for the MSRR-1 configuration is a commercial device supplied by MSFC's Space Products Department (SPD). It includes capabilities for vapor transport processes and liquid metal sintering. This Experiment Module will be replaced on-orbit with other NASA Materials Science EMs.

  3. Effects of suspension of air-conditioning on airtight-type racks.

    Science.gov (United States)

    Kanzaki, M; Fujieda, M; Furukawa, T

    2001-10-01

    Although isolation racks are superior to open-type racks in terms of securing breeding conditions for laboratory animals, the contingency-proofing capability of the former has yet to be determined. Therefore, from the view of risk management, we studied the environmental change in isolation racks by forcibly suspending ventilation and air-conditioning and confirming the maximal time length for complete recovery to the original condition after restarting their operations. The isolation racks were placed in a room that was equipped with an independent air-conditioning system. When the inside condition of the racks reached 22-24 degrees C and 59-64% of relative humidity, the air-conditioning and ventilation were forcibly suspended and the subsequent temperature, relative humidity, ammonium and CO2 concentrations in the racks were measured over time. We found that after suspending the air-conditioning and ventilation, it took 40-60 min for temperature, and about 10 min for relative humidity to exceed the maximum values (temperature and relative humidity) referred to in the Showa 58 Nenban Guideline Jikken Doubutsu Shisetsu no Kenchiku oyobi Setsubi (Guidelines of buildings and facilities for experimental animals in Japan; Year 1983 edition). After 17 hr 25 min of the suspension of air-conditioning and ventilation, two rats were found dead. Then, the air-conditioning and ventilation were restarted. It took about 2 hr for temperature, and 50 min for relative humidity to regain the guideline values. The ammonium concentration stayed within the guideline value with a maximum concentration of 2 ppm in the experimental period, whereas the CO2 concentration was found to exceed 9% at the time of animal death.

  4. Furniture Rack Corrosion Coupon Surveillance - 2012 Update

    International Nuclear Information System (INIS)

    Mickalonis, J. I.; Murphy, T. R.; Berry, C. J.

    2012-01-01

    Under the L Basin corrosion surveillance program furniture rack coupons immersed for 14 years (FY2009 coupons) and 16 years (FY2011 coupons) were analyzed and the results trended with coupons exposed for shorter times. In addition, a section harvested from an actual furniture rack that was immersed for 14 years was analyzed for pitting in the weld and heat-affected-zone (HAZ) regions. The L Basin operations maintained very good water quality over the entire immersion period for these samples. These results for FY2009 and FY2011 coupons showed that the average pit depths for the 6061 and 6063 base metal are 1 and 2 mils, respectively, while those for the weld and HAZ are 3 and 4 mils, respectively. The results for the weld and HAZ regions are similar to coupons removed during the period of FY2003 to FY2007. These similarities indicate that the pit development occurred quickly followed by slow kinetics of increase in pit depth. For the actual furniture rack sample average pits of 5 and 2 mils were measured for the HAZ and weld, respectively. These results demonstrate that pitting corrosion of the aluminum furniture racks used to support the spent fuel occurs in waters of good quality. The corrosion kinetics or pit depth growth rate is much less that 1 mil/year, and would not impact long-term use of this material system for fuel storage racks in L Basin if good water quality is maintained

  5. Antifungal activity of streptomycetes isolated bentonite clay

    Directory of Open Access Journals (Sweden)

    V. P. Shirobokov

    2016-12-01

    Full Text Available Aim. To investigate the biological activity of streptomycetes, isolated from Ukrainian bentonite clay. Methods. For identification of the investigated microorganisms there were used generally accepted methods for study of morpho-cultural and biochemical properties and sequencing of 16Ѕ rRNA producer. Antagonistic activity of the strain was determined by agar diffusion and agar block method using gram-positive, gram-negative microorganisms and fungi. Results. Research of autochthonous flora from bentonite clay of Ukrainian various deposits proved the existence of stable politaxonomic prokaryotic-eukaryotic consortia there. It was particularly interesting that the isolated microorganisms had demonstrated clearly expressed antagonistic properties against fungi. During bacteriological investigation this bacterial culture was identified like representative of the genus Streptomyces. Bentonite streptomycetes, named as Streptomyces SVP-71, inagar mediums (agar block method inhibited the growth of fungi (yeast and mold; zones of growth retardation constituted of 11-36 mm, and did not affect the growth of bacteria. There were investigated the inhibitory effects of supernatant culture fluid, ethanol and butanol extracts of biomass streptomycetes on museum and clinical strains of fungi that are pathogenic for humans (Candida albicans, C. krusei, C. utilis, C. parapsilosis, C. tropicalis, C. kefir, S. glabrata, C. lusitaniae, Aspergillus niger, Mucor pusillus, Fusarium sporotrichioides. It has been shown that research antifungal factor had 100% of inhibitory effect against all fungi used in experiments in vitro. In parallel, it was found that alcohol extracts hadn’t influence to the growth of gram-positive and gram-negative bacteria absolutely. It was shown that the cultural fluid supernatant and alcoholic extracts of biomass had the same antagonistic effect, but with different manifestation. This evidenced about identity of antifungal substances

  6. Laboratory glassware rack for seismic safety

    Science.gov (United States)

    Cohen, M. M. (Inventor)

    1985-01-01

    A rack for laboratory bottles and jars for chemicals and medicines has been designed to provide the maximum strength and security to the glassware in the event of a significant earthquake. The rack preferably is rectangular and may be made of a variety of chemically resistant materials including polypropylene, polycarbonate, and stainless steel. It comprises a first plurality of parallel vertical walls, and a second plurality of parallel vertical walls, perpendicular to the first. These intersecting vertical walls comprise a self-supporting structure without a bottom which sits on four legs. The top surface of the rack is formed by the top edges of all the vertical walls, which are not parallel but are skewed in three dimensions. These top edges form a grid matrix having a number of intersections of the vertical walls which define a number of rectangular compartments having varying widths and lengths and varying heights.

  7. 'Swab racks are an old fashioned idea'.

    Science.gov (United States)

    Mumford, M

    1991-12-01

    Mary Mumford, theatre sister at the Princes of Wales Hospital, Bridgend, was asked to speak in a short debate at an NATN branch meeting, supporting the motion that 'swab racks are an old fashioned idea'. Although she did not like swab racks she had not attempted thus far to do anything about them. In the event, she actually lost the debate--not in principle but because she could offer no effective alternative method of checking swabs. Having been given the incentive, a trial is now being conducted in her hospital similar to that described by Paul Wicker. This is the case presented by Mary Mumford supporting the following motion ... 'that swab racks are an old fashioned idea, which cause more potential problems due to exposure of blood than is proven to be safe in today's theatre environment'.

  8. Rack for storage of nuclear fuel elements

    International Nuclear Information System (INIS)

    Bosshart, E.

    1987-01-01

    The rack contains a cluster of parallel, approximately vertical, neutron absorbing square section tubes which are attached to a bottom plate and mutually supported along their edges using welded joining pieces. These welded pieces touch each other laterally in at least one middle plane where the rack is joined. The pieces are additionally secured laterally by screws. Thereby a safe and simple mutual support of the tubes is achieved. It is possible to dismount the tubes by releasing the screws without the necessity to move the tubes in a horizontal direction. 4 figs

  9. 30 CFR 56.7050 - Tool and drill steel racks.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Tool and drill steel racks. 56.7050 Section 56.7050 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL... Jet Piercing Drilling § 56.7050 Tool and drill steel racks. Receptacles or racks shall be provided for...

  10. 40 CFR 63.650 - Gasoline loading rack provisions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 10 2010-07-01 2010-07-01 false Gasoline loading rack provisions. 63... loading rack provisions. (a) Except as provided in paragraphs (b) through (c) of this section, each owner or operator of a Group 1 gasoline loading rack classified under Standard Industrial Classification...

  11. 30 CFR 57.7050 - Tool and drill steel racks.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Tool and drill steel racks. 57.7050 Section 57... Rotary Jet Piercing Drilling-Surface and Underground § 57.7050 Tool and drill steel racks. Receptacles or racks shall be provided for drill steel and tools stored or carried on drills. ...

  12. Nuclear reactor spent fuel storage rack

    International Nuclear Information System (INIS)

    Machado, O.J.; Flynn, W.M.; Flanders, H.E. Jr.; Booker, L.W.

    1989-01-01

    A fuel rack is described for use in storing nuclear fuel assemblies in a nuclear fuel storage pool having a floor on which an upwardly projecting stud is mounted; the fuel rack comprising: a base structure at the lower end of the fuel rack including base-plate means having flow openings therein, the base-plate means supporting a first network of interlaced beams which form a multiplicity of polygonal openings; a second network of interlaced beams forming polygonal openings positioned in spaced vertical alignment with corresponding polygonal openings in the first network of beams; a plurality of cells, each cell having sides bounded by inner and outer surfaces and being of a size and configuration designed to hold therein a fuel assembly, each cell positioned in a corresponding pair of the aligned polygonal openings, each cell being open at both ends with a guiding funnel at the upper end, and the cells being positioned over the flow openings in the base-plate to permit flow of coolant through the cells; spaced, outwardly directed, projections on the outer surfaces of the sides of the cells near the tops and bottoms of the sides thereof, each cell being sized to be received within a corresponding of the pair of aligned polygonal openings in which the cells are respectively positioned; and means fixedly securing the projections to the beams in the first and second networks of beams thereby to provide a substantially rigid fuel rack of modular design

  13. Flow field mapping in data rack model

    Directory of Open Access Journals (Sweden)

    Matěcha J.

    2013-04-01

    Full Text Available The main objective of this study was to map the flow field inside the data rack model, fitted with three 1U server models. The server model is based on the common four-processor 1U server. The main dimensions of the data rack model geometry are taken fully from the real geometry. Only the model was simplified with respect to the greatest possibility in the experimental measurements. The flow field mapping was carried out both experimentally and numerically. PIV (Particle Image Velocimetry method was used for the experimental flow field mapping, when the flow field has been mapped for defined regions within the 2D/3D data rack model. Ansys CFX and OpenFOAM software were used for the numerical solution. Boundary conditions for numerical model were based on data obtained from experimental measurement of velocity profile at the output of the server mockup. This velocity profile was used as the input boundary condition in the calculation. In order to achieve greater consistency of the numerical model with experimental data, the numerical model was modified with regard to the results of experimental measurements. Results from the experimental and numerical measurements were compared and the areas of disparateness were identified. In further steps the obtained proven numerical model will be utilized for the real geometry of data racks and data.

  14. RackSaver neutron absorbing device development and testing

    International Nuclear Information System (INIS)

    Lambert, R.; O'Leary, P.; Roberts, P.

    1996-01-01

    Siemens Power Corporation (SPC), in cooperation with the Electric Power Research Institute (EPRI), has developed the RackSaver neutron absorbing insert. The RackSaver insert can be installed onto spent nuclear fuel assemblies to replace deteriorating Boraflex neutron absorbing material installed in some spent-fuel storage racks. This paper describes results of a development and in-pool demonstration program performed to support potential utilization of the RackSaver neutron absorbing insert by affected utilities. The program objective was to advance the RackSaver concept into a field-demonstrated product. This objective was accomplished through three phases: design, licensing and criticality evaluations, and demonstration testing

  15. Kale BoRACK1 is involved in the plant response to salt stress and Peronospora brassicae Gaumann.

    Science.gov (United States)

    Li, Da-Hong; Shen, Fu-Jia; Li, Hong-Yan; Li, Wei

    2017-06-01

    The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, a kale (Brassica oleracea var. acephala f.tricolor) RACK1 gene (BoRACK1) was cloned by RT-PCR. The amino acid sequence of BoRACK1 had seven WD repeats in which there were typical GH (glycine-histidine) and WD dipeptides. Comparison with AtRACK1 from Arabidopsis revealed 87.1% identity at the amino acid level. Expression pattern analysis by RT-PCR showed that BoRACK1 was expressed in all analyzed tissues of kale and that its transcription in leaves was down-regulated by salt, abscisic acid, and H 2 O 2 at a high concentration. Overexpression of BoRACK1 in kale led to a reduction in symptoms caused by Peronospora brassicae Gaumann on kale leaves. The expression levels of the pathogenesis-related protein genes, PR-1 and PRB-1, increased 2.5-4-fold in transgenic kale, and reactive oxygen species production was more active than in the wild-type. They also exhibited increased tolerance to salt stress in seed germination. H 2 O 2 may also be involved in the regulation of BoRACK1 during seed germination under salt stress. Quantitative real-time PCR analyses showed that the transcript levels of BoRbohs genes were significantly higher in overexpression of BoRACK1 transgenic lines. Yeast two-hybrid assays showed that BoRACK1 could interact with WNK8, eIF6, RAR1, and SGT1. This study and previous work lead us to believe that BoRACK1 may form a complex with regulators of plant salt and disease resistance to coordinate kale reactions to pathogens. Copyright © 2017 Elsevier GmbH. All rights reserved.

  16. Antimicrobial activity of Streptomyces spp. Isolates from vegetable plantation soil

    Directory of Open Access Journals (Sweden)

    Isnaeni

    2016-05-01

    Full Text Available Fifteen Streptomyces isolates were isolated from soil in some different location on vegetable plantation at agriculture standard condition. The isolates were assessed for their antibacterial activity against Mycobacterium tuberculosis (MTB ATCC H37RV and mycobacterial which isolated from Dr. Soetomo Hospital patients in Surabaya. The International Streptomyces Project 4 (ISP4 and Middlebrook 7H9 (MB7H9 wwere used as growth or fermentation medium. The screening of inhibition activity was performed using turbidimetry and spot-test on agar medium. Results shown that 33.3% of the isolates (5 isolates have anti-mycobacterial activities. The first line anti tuberculosis drug rifampicin, (RIF, ethambutol (EMB, isoniazid (INH, and pyrazinamide (PZA were used as standards or positive controls with concentration 20 ppm. Optical density of crude fermentation broth concentrated from five isolates relatively lower than five anti-tuberculosis drug activity standard, although their activities against some microbial were similar to the standard at spot-test. The most efficient isolate shown anti-mycobacterial activity was Streptomyces B10 which identified as Streptomyces violaceousniger. In addition, fatty acid methyl ester (FAME profile of gas chromatography-mass spectrometry chromatogram of each isolates were studied and compared to Streptomyces spp. Keywords: Anti-mycobacterial, Mycobacterium tuberculosis, Streptomyces spp.

  17. The scaffold protein RACK1 is a target of endocrine disrupting chemicals (EDCs) with important implication in immunity

    Energy Technology Data Exchange (ETDEWEB)

    Buoso, Erica; Galasso, Marilisa; Ronfani, Melania [Dipartimento di Scienze del Farmaco, Università degli Studi di Pavia, Viale Taramelli 12/14, 27100 Pavia (Italy); Papale, Angela; Galbiati, Valentina [Laboratory of Toxicology, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Via Balzaretti 9, 20133 Milano (Italy); Eberini, Ivano [Laboratorio di Biochimica e Biofisica Computazionale, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan (Italy); Marinovich, Marina [Laboratory of Toxicology, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Via Balzaretti 9, 20133 Milano (Italy); Racchi, Marco [Dipartimento di Scienze del Farmaco, Università degli Studi di Pavia, Viale Taramelli 12/14, 27100 Pavia (Italy); Corsini, Emanuela, E-mail: emanuela.corsini@unimi.it [Laboratory of Toxicology, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Via Balzaretti 9, 20133 Milano (Italy)

    2017-06-15

    We recently demonstrated the existence of a complex hormonal balance between steroid hormones in the control of RACK1 (Receptor for Activated C Kinase 1) expression and immune activation, suggesting that this scaffold protein may also be targeted by endocrine disrupting chemicals (EDCs). As a proof of concept, we investigated the effect of the doping agent nandrolone, an androgen receptor (AR) agonist, and of p,p′DDT (dichlorodiphenyltrichloroethane) and its main metabolite p,p′DDE (dichlorodiphenyldichloroethylene), a weak and strong AR antagonist, respectively, on RACK1 expression and innate immune response. In analogy to endogenous androgens, nandrolone induced a dose-related increase in RACK1 transcriptional activity and protein expression, resulting in increased LPS-induced IL-8 and TNF-α production and proliferation in THP-1 cells. Conversely, p,p′DDT and p,p′DDE significantly decrease RACK1 expression, LPS-induced cytokine production and CD86 expression; with p,p′DDE exerting a stronger repressor effect than p,p′DDT, consistent with its stronger AR antagonistic effect. These results indicate that RACK1 could be a relevant target of EDCs, responding in opposite ways to agonist or antagonist of AR, representing a bridge between the endocrine system and the innate immune system. - Highlights: • RACK1 expression can be induced by AR agonists with a consequent enhancement of the response to LPS. • RACK1 can be negatively modulated by the AR antagonists DDT and its main metabolite p,p′DDE. • RACK1 can be a relevant target of EDCs, representing a bridge between the endocrine system and the immune system.

  18. OsRACK1 Is Involved in Abscisic Acid- and H2O2-Mediated Signaling to Regulate Seed Germination in Rice (Oryza sativa, L.)

    Science.gov (United States)

    Zhang, Dongping; Chen, Li; Li, Dahong; Lv, Bing; Chen, Yun; Chen, Jingui; XuejiaoYan; Liang, Jiansheng

    2014-01-01

    The receptor for activated C kinase 1 (RACK1) is one member of the most important WD repeat–containing family of proteins found in all eukaryotes and is involved in multiple signaling pathways. However, compared with the progress in the area of mammalian RACK1, our understanding of the functions and molecular mechanisms of RACK1 in the regulation of plant growth and development is still in its infancy. In the present study, we investigated the roles of rice RACK1A gene (OsRACK1A) in controlling seed germination and its molecular mechanisms by generating a series of transgenic rice lines, of which OsRACK1A was either over-expressed or under-expressed. Our results showed that OsRACK1A positively regulated seed germination and negatively regulated the responses of seed germination to both exogenous ABA and H2O2. Inhibition of ABA biosynthesis had no enhancing effect on germination, whereas inhibition of ABA catabolism significantly suppressed germination. ABA inhibition on seed germination was almost fully recovered by exogenous H2O2 treatment. Quantitative analyses showed that endogenous ABA levels were significantly higher and H2O2 levels significantly lower in OsRACK1A-down regulated transgenic lines as compared with those in wildtype or OsRACK1A-up regulated lines. Quantitative real-time PCR analyses showed that the transcript levels of OsRbohs and amylase genes, RAmy1A and RAmy3D, were significantly lower in OsRACK1A-down regulated transgenic lines. It is concluded that OsRACK1A positively regulates seed germination by controlling endogenous levels of ABA and H2O2 and their interaction. PMID:24865690

  19. Bacterial isolates from the bryozoan Membranipora membranacea: influence of culture media on isolation and antimicrobial activity.

    Science.gov (United States)

    Heindl, Herwig; Thiel, Vera; Wiese, Jutta; Imhoff, Johannes F

    2012-03-01

    From specimens of the bryozoan Membranipora membranacea collected in the Baltic Sea, bacteria were isolated on four different media, which significantly increased the diversity of the isolated groups. All isolates were classified according to 16S rRNA gene sequence analysis and tested for antimicrobial properties using a panel of five indicator strains and six different media. Each medium featured a unique set of isolated phylotypes, and a phylogenetically diverse collection of isolates was obtained. A total of 96 isolates were assigned to 49 phylotypes and 29 genera. Only one-third of the members of these genera had been isolated previously from comparable sources. The isolates were affiliated with Alpha- and Gammaproteobacteria, Bacilli, and Actinobacteria. A comparable large portion of up to 22 isolates, i.e., 15 phylotypes, probably represent new species. Likewise, 47 isolates (approximately 50%) displayed antibiotic activities, mostly against grampositive indicator strains. Of the active strains, 63.8 % had antibiotic traits only on one or two of the growth media, whereas only 12.7 % inhibited growth on five or all six media. The application of six different media for antimicrobial testing resulted in twice the number of positive hits as obtained with only a single medium. The use of different media for the isolation of bacteria as well as the variation of media considered suitable for the production of antibiotic substances significantly enhanced both the number of isolates obtained and the proportion of antibiotic active cultures. Thus the approach described herein offers an improved strategy in the search for new antibiotic compounds.

  20. Seismic analysis of spent nuclear fuel storage racks

    International Nuclear Information System (INIS)

    Shah, S.J.; Biddle, J.R.; Bennett, S.M.; Schechter, C.B.; Harstead, G.A.; Marquet, F.

    1996-01-01

    In many nuclear power plants, existing storage racks are being replaced with high-density racks to accommodate the increasing inventory of spent fuel. In the hypothetical design considered here, the high-density arrangement of fuel assemblies, or consolidated fuel canisters, is accomplished through the use of borated stainless steel (BSS) plates acting as neutron absorbers. No structural benefit from the BSS is assumed. This paper describes the methods used to perform seismic analysis of high density spent fuel storage racks. The sensitivity of important parameters such as the effect of variation of coefficients of friction between the rack legs and the pool floor and fuel loading conditions (consolidated and unconsolidated) are also discussed in the paper. Results of this study are presented. The high-density fuel racks are simply supported by the pool floor with no structural connections to adjacent racks or to the pool walls or floor. Therefore, the racks are free standing and may slide and tip. Several time history, nonlinear, seismic analyses are required to account for variations in the coefficient of friction, rack loading configuration, and the type of the seismic event. This paper presents several of the mathematical models usually used. Friction cannot be precisely predicted, so a range of friction coefficients is assumed. The range assumed for the analysis is 0.2 to 0.8. A detailed model representing a single rack is used to evaluate the 3-D loading effects. This model is a controlling case for the stress analysis. A 2-D multi-rack model representing a row of racks between the spent fuel pool walls is used to evaluate the change in gaps between racks. The racks are normally analyzed for the fuel loading conditions of consolidated, full, empty, and half-loaded with fuel assemblies

  1. TCR triggering induces the formation of Lck-RACK1-actinin-1 multiprotein network affecting Lck redistribution

    Directory of Open Access Journals (Sweden)

    Ondrej Ballek

    2016-10-01

    Full Text Available The initiation of T-cell signaling is critically dependent on the function of the member of Src family tyrosine kinases (SFKs, Lck. Upon TCR triggering, Lck kinase activity induces the nucleation of signal-transducing hubs that regulate the formation of complex signaling network and cytoskeletal rearrangement. In addition, the delivery of Lck function requires rapid and targeted membrane redistribution, but the mechanism underpinning this process is largely unknown. To gain insight into this process, we considered previously described proteins that could assist in this process via their capacity to interact with kinases and regulate their intracellular translocations. An adaptor protein, Receptor for Activated C Kinase 1 (RACK1, was chosen as a viable option and its capacity to bind Lck and aid the process of activation-induced redistribution of Lck was assessed. Our microscopic observation showed that T-cell activation induces a rapid, concomitant and transient co-redistribution of Lck and RACK1 into the forming immunological synapse. Consistent with this observation, the formation of transient RACK1-Lck complexes were detectable in primary CD4+ T-cells with their maximum levels peaking 10 seconds after TCR-CD4 co-aggregation. Moreover, RACK1 preferentially binds to a pool of kinase active pY394Lck which co-purifies with high molecular weight cellular fractions. The formation of RACK1-Lck complexes depends on functional SH2 and SH3 domains of Lck and includes several other signaling and cytoskeletal elements that transiently bind the complex. Notably, the F-actin-crosslinking protein, α-actinin-1, binds to RACK1 only in the presence of kinase active Lck suggesting that the formation of RACK1-pY394Lck-α-actinin-1 complex serves as a signal module coupling actin cytoskeleton bundling with productive TCR/CD4 triggering. In addition, the treatment of CD4+ T-cells with nocodazole, which disrupts the microtubular network, also blocked the formation

  2. Definition of a RACK1 Interaction Network in Drosophila melanogaster Using SWATH-MS.

    Science.gov (United States)

    Kuhn, Lauriane; Majzoub, Karim; Einhorn, Evelyne; Chicher, Johana; Pompon, Julien; Imler, Jean-Luc; Hammann, Philippe; Meignin, Carine

    2017-07-05

    Receptor for Activated protein C kinase 1 (RACK1) is a scaffold protein that has been found in association with several signaling complexes, and with the 40S subunit of the ribosome. Using the model organism Drosophila melanogaster , we recently showed that RACK1 is required at the ribosome for internal ribosome entry site (IRES)-mediated translation of viruses. Here, we report a proteomic characterization of the interactome of RACK1 in Drosophila S2 cells. We carried out Label-Free quantitation using both Data-Dependent and Data-Independent Acquisition (DDA and DIA, respectively) and observed a significant advantage for the Sequential Window Acquisition of all THeoretical fragment-ion spectra (SWATH) method, both in terms of identification of interactants and quantification of low abundance proteins. These data represent the first SWATH spectral library available for Drosophila and will be a useful resource for the community. A total of 52 interacting proteins were identified, including several molecules involved in translation such as structural components of the ribosome, factors regulating translation initiation or elongation, and RNA binding proteins. Among these 52 proteins, 15 were identified as partners by the SWATH strategy only. Interestingly, these 15 proteins are significantly enriched for the functions translation and nucleic acid binding. This enrichment reflects the engagement of RACK1 at the ribosome and highlights the added value of SWATH analysis. A functional screen did not reveal any protein sharing the interesting properties of RACK1, which is required for IRES-dependent translation and not essential for cell viability. Intriguingly however, 10 of the RACK1 partners identified restrict replication of Cricket paralysis virus (CrPV), an IRES-containing virus. Copyright © 2017 Kuhn et al.

  3. Sensor fusion for active vibration isolation in precision equipment

    NARCIS (Netherlands)

    Tjepkema, D.; van Dijk, Johannes; Soemers, Herman

    2012-01-01

    Sensor fusion is a promising control strategy to improve the performance of active vibration isolation systems that are used in precision equipment. Normally, those vibration isolation systems are only capable of realizing a low transmissibility. Additional objectives are to increase the damping

  4. Activity of daptomycin against Listeria monocytogenes isolates from cerebrospinal fluid

    NARCIS (Netherlands)

    Spanjaard, Lodewijk; Vandenbroucke-Grauls, Christina M. J. E.

    2008-01-01

    We tested the activity of daptomycin against 76 Listeria monocytogenes isolates from cerebrospinal fluid by broth dilution and Etest methods. For the broth dilution method, the MIC range was 1.0 to 8.0 and the MIC at which 90% of the isolates tested were inhibited (MIC(90)) was 4.0 mg/liter. For the

  5. Spent fuel storage rack for atomic power plant

    International Nuclear Information System (INIS)

    Kodama, Tatemitsu.

    1981-01-01

    Purpose: To flexibly cope with the changes in the size and shape of spent fuel storage containers by placing a number of independently-constructed rack cells in a rack frame in such a manner that the guide support members of the storage rack, mounted on each rack cell may be replaced. Constitution: Independently-constructed rack cells are inserted from above into a rack frame rigidly installed on the bottom of a water pool. Each cell is produced by welding, has a handling head mounted at the top, and guide support members made of three replaceable guide tubes are mounted with bolts. If the size and the shape of the containers are altered, this configuration can easily cope with the new container shape by merely having the guide tubes replaced, without adversely affecting other cells and without necessitating draining of the water in the pool. (Yoshino, Y.)

  6. Casimir rack and pinion as a miniaturized kinetic energy harvester

    Science.gov (United States)

    Miri, MirFaez; Etesami, Zahra

    2016-08-01

    We study a nanoscale machine composed of a rack and a pinion with no contact, but intermeshed via the lateral Casimir force. We adopt a simple model for the random velocity of the rack subject to external random forces, namely, a dichotomous noise with zero mean value. We show that the pinion, even when it experiences random thermal torque, can do work against a load. The device thus converts the kinetic energy of the random motions of the rack into useful work.

  7. Isolation and Identification of Active Compounds from Papaya Plants and Activities as Antimicrobial

    Science.gov (United States)

    Prasetya, A. T.; Mursiti, S.; Maryan, S.; Jati, N. K.

    2018-04-01

    Extraction and isolation of papaya seeds and leaves (Carica papaya L) has been performed using n-hexane and ethanol solvents. Further isolation of the extract obtained using ethyl acetate and diethyl ether solvents. The result of the phytochemical test of papaya extract obtained by mixture of an active compound of flavonoids, alkaloids, tannins, steroids, and saponins. Ethyl acetate isolates containing only flavonoids and diethyl ether isolates contain only alkaloids. Extracts and isolates from papaya plants had gram-positive antibacterial activity greater than the gram-negative bacteria, but both did not have antifungal activity. Papaya extracts have greater antibacterial activity than flavonoid isolates and alkaloid isolates. Strong antibacterial inhibitory sequences are extracts of papaya plants, flavonoid isolates, and alkaloid isolates.

  8. Screening of Azotobacter isolates for PGP properties and antifungal activity

    Directory of Open Access Journals (Sweden)

    Bjelić Dragana Đ.

    2015-01-01

    Full Text Available Аmong 50 bacterial isolates obtained from maize rhizospherе, 13 isolates belonged to the genus Azotobacter. Isolates were biochemically characterized and estimated for pH and halo tolerance ability and antibiotic resistance. According to characterization, the six representative isolates were selected and further screened in vitro for plant growth promoting properties: production of indole-3-acetic acid (IAA, siderophores, hydrogen cyanide (HCN, exopolysaccharides, phosphate solubilization and antifungal activity (vs. Helminthosporium sp., Macrophomina sp., Fusarium sp.. Beside HCN production, PGP properties were detected for all isolates except Azt7. All isolates produced IAA in the medium without L-tryptophan and the amount of produced IAA increased with concentration of precursor in medium. The highest amount of IAA was produced by isolates Azt4 (37.69 and 45.86 μg ml-1 and Azt5 (29.44 and 50.38 μg ml-1 in the medium with addition of L-tryptophan (2.5 and 5 mM. The isolates showed the highest antifungal activity against Helminthosporium sp. and the smallest antagonistic effect on Macrophomina sp. Radial Growth Inhibition (RGI obtained by the confrontation of isolates with tested phytopathogenic fungi, ranged from 10 to 48%. [Projekat Ministarstva nauke Republike Srbije, br. TR 31073

  9. Active vibration isolation of a rigidly mounted turbo pump

    NARCIS (Netherlands)

    Basten, T.G.H.; Doppenberg, E.J.J.

    2006-01-01

    Manufacturers of precision equipment are constantly aiming at increased accuracy. Elimination of disturbing vibrations is therefore getting more and more important. The technical limitations of passive isolation methods require alternative strategies for vibration reduction, such as active

  10. Isolation of antifungally active lactobacilli from edam cheese

    DEFF Research Database (Denmark)

    Tuma, S.; Vogensen, Finn Kvist; Plocková, M.

    2007-01-01

    The antifungal activity of 322 lactobacilli strains isolated from Edam cheese at different stages of the ripening process was tested against Fusarium proliferatum M 5689 using a dual overlay spot assay. Approximately 21% of the isolates showed a certain level of inhibitory activity. Seven strains...... as Lb. paracasei and three as Lb. fermentum. Lb. paracasei ST 68 was chosen for further testing as antifungal protective adjunct for Edam cheese production.  ...

  11. Isolation, characterization and antimicrobial activity of Streptomyces ...

    African Journals Online (AJOL)

    DR TONUKARI

    2013-12-18

    Dec 18, 2013 ... Available online at http://www.academicjournals.org/AJB ... Key words: Characterization, streptomyces, antimicrobial activity, hot ... MATERIALS AND METHODS ..... chain reaction (PCR) which is currently used as a sen-.

  12. Storage racks for spent nuclear fuels

    International Nuclear Information System (INIS)

    Matsumoto, Takashi; Ukaji, Hideo; Okino, Yoshiyuki; Ishihara, Jo; Ikuta, Isao.

    1983-01-01

    Purpose: To facilitate the mounting of neutron absorbers made of amorphous alloys to fuel racks. Constitution: Neutron absorbers are mounted to a cylindrical member of a square cross section for containing to retain spent fuels only on paired opposing sides by means of machine screws or the likes. Then, such cylindrical members are disposed so that their sides attached with the neutron absorbers are not in adjacent with each other. In this way, mounting of the neutron absorbers over the entire surface of the cylindrical members is no more necessary thereby enabling to simplify the mounting work. (Ikeda, J.)

  13. Antifungal activity of indigenous Bacillus spp. isolated from soil

    Directory of Open Access Journals (Sweden)

    Bjelić Dragana Đ.

    2017-01-01

    Full Text Available Biocontrol using plant growth-promoting rhizobacteria (PGPR represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2, Bacillus pumilus (B3, B11, Bacillus subtilis (B5, B7 and Bacillus megaterium (B8, B9. The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth and B7 (from 40% to 71% reduction in fungal growth. These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR-31073

  14. Passive and active vibration isolation systems using inerter

    Science.gov (United States)

    Alujević, N.; Čakmak, D.; Wolf, H.; Jokić, M.

    2018-03-01

    This paper presents a theoretical study on passive and active vibration isolation schemes using inerter elements in a two degree of freedom (DOF) mechanical system. The aim of the work is to discuss basic capabilities and limitations of the vibration control systems at hand using simple and physically transparent models. Broad frequency band dynamic excitation of the source DOF is assumed. The purpose of the isolator system is to prevent vibration transmission to the receiving DOF. The frequency averaged kinetic energy of the receiving mass is used as the metric for vibration isolation quality. It is shown that the use of inerter element in the passive vibration isolation scheme can enhance the isolation effect. In the active case, a feedback disturbance rejection scheme is considered. Here, the error signal is the receiving body absolute velocity which is directly fed to a reactive force actuator between the source and the receiving bodies. In such a scheme, the so-called subcritical vibration isolation problems exist. These problems are characterised by the uncoupled natural frequency of the receiving body larger than the uncoupled natural frequency of the source body. In subcritical vibration isolation problems, the performance of the active control is limited by poor stability margins. This is because the stable feedback gain is restricted in a narrow range between a minimum and a maximum. However, with the inclusion of an inerter in the isolator, one of the two stability margins can be opened. This enables large, theoretically unlimited negative feedback gains and large active damping of the receiving body vibration. A simple expression for the required inertance is derived.

  15. Refurbishment of the Oregon State University rotating rack

    International Nuclear Information System (INIS)

    Higginbotham, J.F.

    1991-01-01

    TRIGA reactors have experienced operational difficulties with the rotating racks used for sample irradiation. The most common problem occurs when the rack seizes, and the corrective action taken is replacement of the rack assembly. This paper describes the symptoms leading to rack failure and a refurbishment procedure to correct the problem without replacing the rack at the Oregon State University TRIGA Reactor (OSTR) Facility. This procedure was accomplished with extraordinary results from an operational and a radiation protection standpoint. The refurbishment has extended the useful life of this reactor facility with minimal financial impact. Given the declining number of university-based research reactors, it is in the nation's best interest to maintain the currently operating research reactor facilities, and the described procedure can aid in achieving that goal

  16. In vitro activity of difloxacin against canine bacterial isolates

    NARCIS (Netherlands)

    Hoven, van den J.R.; Wagenaar, J.A.; Walker, R.D.

    2000-01-01

    The in vitro activity of difloxacin against canine bacterial isolates from clinical cases was studied in the United States and The Netherlands. Minimal inhibitory concentrations (MIC), the postantibiotic effect, the effect of pH on antimicrobial activity, and the bacterial killing rate tests were

  17. Isolation of a tyrosine-activating enzyme from baker's yeast

    NARCIS (Netherlands)

    Ven, A.M. van de; Koningsberger, V.V.; Overbeek, J.Th.G.

    1958-01-01

    The extracts of ether-CO2-frozen baker's yeast contain enzymes that catalyze the ATP-linked amino acid activation by way of pyrophosphate elimination. From the extract a tyrosine-activating enzyme could be isolated, which, judging from ultracentrifugation and electrophoretic data, was about 70% pure

  18. Acute effects of active isolated stretching on vertical jump ...

    African Journals Online (AJOL)

    The purpose of the study was to determine the acute effects of active isolated stretching on muscular peak power production. Sixty healthy, physically active volunteers (aged 18-28) participated as subjects in this study. Subjects were randomly assigned to two groups; the control group and the experimental group. Subjects ...

  19. Arginase activity of Leishmania isolated from patients with cutaneous leishmaniasis.

    Science.gov (United States)

    Badirzadeh, A; Taheri, T; Abedi-Astaneh, F; Taslimi, Y; Abdossamadi, Z; Montakhab-Yeganeh, H; Aghashahi, M; Niyyati, M; Rafati, S

    2017-09-01

    Cutaneous leishmaniasis (CL) is one of the most important vector-borne parasitic diseases, highly endemic in Iran, and its prevalence is increasing all over the country. Arginase (ARG) activity in isolated Leishmania parasites from CL patients is yet to be explored. This study aimed to compare the ARG activity of isolated Leishmania promastigotes from CL patients with a standard strain of Leishmania major and its influences on the disease pathogenesis. We recruited 16 confirmed CL patients from Qom Province, in central Iran; after detection of Leishmania species using PCR-RFLP, we assessed the levels of ARG in the isolated promastigotes and determined the parasites' growth rate. Only L. major was identified from CL patients. The level of ARG activity in the isolated Leishmania promastigotes from CL patients was significantly higher than that obtained from the standard strain of L. major. No significant correlations between ARG activity and lesion size, number or duration were observed; in contrast, a significant negative correlation was seen between ARG level and Leishmania' growth rate. The obtained results suggest that increased ARG expression and activity in the isolated Leishmania promastigotes might contribute to the higher parasite infectivity and play a major role in the pathogenicity of the CL. © 2017 John Wiley & Sons Ltd.

  20. Habitat use by Red Knots (Calidris canutus rufa): Experiments with oyster racks and reefs on the beach and intertidal of Delaware Bay, New Jersey

    Science.gov (United States)

    Burger, Joanna; Niles, Lawrence J.

    2017-07-01

    Sea level rise and increasing human activities have decreased intertidal habitat in many places in the world. The expansion of aquaculture in intertidal areas may impact birds and other organisms using these habitats, leading to questions of sustainability of both aquaculture and functioning estuarine ecosystems. Understanding the effect of oyster culture on shorebird activity, particularly on Red Knots (Calidris canutus rufa), a species on the U.S. Threatened List, is important for adaptive management and the expansion of oyster culture. In May 2013 we experimentally compared Red Knot and shorebird use of a beach section with racks and a control, and in 2016 we compared the use of sections with artificial reefs, oyster racks, and control on Delaware Bay, New Jersey (USA). The data included only times when no workers or other people were present. Censuses, conducted every 30 min throughout the day (279 censuses in 2013, 231 censuses in 2016), included the number of Red Knots and other shorebirds in each treatment section. In 2013, the total number of shorebirds was significantly higher in the rack section than in the control section, except for Red Knots and Ruddy Turnstones (Arenaria interpres) that occurred in higher numbers in the control than in the rack section. In 2016 Red Knot numbers were also significantly lower in the rack section. In 2013, the mean number of Red Knots/census was 13 for racks vs 59 for the control (P racks and over 68 for other treatments (P racks while both foraging and roosting, suggesting that caution should be used before placing oyster racks in areas used for foraging by Red Knots.

  1. Status report on activities on seismic isolation in Italy

    International Nuclear Information System (INIS)

    Martelli, A.; Bettinali, F.

    1992-01-01

    The development of seismic isolation and its application to structures other than bridges were started in Italy in 1988. Considerable efforts are being devoted to this technique, both because it can already be widely used in civil buildings (where it is particularly attractive for constructions that are critical for emergency and disaster planning), and due to the very promising perspectives for application to the industrial plants. In particular, ENEA is also quite interested in verifying the applicability of seismic isolation to the high risk plants, including the innovative nuclear reactors. The correct development of seismic isolation, for a future wide use in all the domains of interest - including high risk and other industrial plants - requires that a sufficient number of applications to civil buildings is -undertaken, so as to improve the knowledge on the design and behaviour of isolated structures. It also requires seismic monitoring of isolated constructions. This is the reason why all the ongoing studies in Italy - including those of ENEA and ENEL - are based at present on applications to civil buildings. To the aforesaid aims, R and D work is also needed: such a work, together with the experience acquired on actual isolated buildings, is essential to set up adequate design rules. On the other hand, development of design rules must be carried out in parallel, in order to determine the features of the necessary research activities. Until now, our development work has been focussed on the high damping steel-laminated rubber bearings, which have been adopted for most isolated buildings in Italy. It consists of: [a] the set-up of proposals for design rules and guidelines; [b] experiments on bearing materials, individual bearings, isolated structure mock-ups, and actual isolated buildings; [c] development and validation of simplified and detailed numerical models of bearings and structures. Furthermore, support is being provided to the designers of isolated

  2. Antiproliferative Activity of Hamigerone and Radicinol Isolated from Bipolaris papendorfii

    Directory of Open Access Journals (Sweden)

    Periyasamy Giridharan

    2014-01-01

    Full Text Available Secondary metabolites from fungi organisms have extensive past and present use in the treatment of many diseases and serve as compounds of interest both in their natural form and as templates for synthetic modification. Through high throughput screening (HTS and bioassay-guided isolation, we isolated two bioactive compounds hamigerone (1 and radicinol (2. These compounds were isolated from fungus Bipolaris papendorfii, isolated from the rice fields of Dera, Himachal Pradesh, India. The structures of the compounds were established on the basis of spectroscopic data, namely, NMR (1H, 13C, mass, and UV. Both compounds were found to be antiproliferative against different cancer cells. Furthermore we have also noted that both compounds showed increase in apoptosis by favorably modulating both tumor suppressor protein (p53 and antiapoptic protein (BCL-2, and in turn increase caspase-3 expression in cancer cells. This is the first report of these compounds from fungus Bipolaris papendorfii and their anticancer activity.

  3. Nonlinear dynamics of a rack-pinion-rack device powered by the Casimir force.

    Science.gov (United States)

    Miri, MirFaez; Nekouie, Vahid; Golestanian, Ramin

    2010-01-01

    Using the lateral Casimir force-a manifestation of the quantum fluctuations of the electromagnetic field between objects with corrugated surfaces-as the main force transduction mechanism, a nanomechanical device with rich dynamical behaviors is proposed. The device is made of two parallel racks that are moving in the same direction and a pinion in the middle that couples with both racks via the noncontact lateral Casimir force. The built-in frustration in the device causes it to be very sensitive and react dramatically to minute changes in the geometrical parameters and initial conditions of the system. The noncontact nature of the proposed device could help with the ubiquitous wear problem in nanoscale mechanical systems.

  4. Storage rack for nuclear fuel assemblies

    International Nuclear Information System (INIS)

    Wachter, W.J.

    1988-01-01

    A storage rack for nuclear fuel assemblies is described comprising storage tubes, each having a polygon cross-section. The tubes being nested with cell walls of one tube aligned with and confronting cell walls of other tubes. Each cell wall having an array of embossed buttons so arranged that buttons of one cell wall engage buttons of a confronting cell wall, and the engage buttons are welded together to secure the tubes. At least one layer of neutron-poison material comprises a flexible, resilient pad interprosed between the aligned cell walls; whereby a major portion of the total outer surface area of each confronting cell wall is engaged with the layer of neutron-poison material

  5. Improvement of rotary specimen rack design

    International Nuclear Information System (INIS)

    Batch, J.M.; Gietzen, A.J.

    1978-01-01

    A redesign and verification test program has been completed on a new Rotary Specimen Rack ('Lazy Susan') design for the TRIGA Mark III. The purpose of the redesign was to solve a rotation problem which occurred at power levels of about 1 MW and above. The previous redesign effort on the Mark II-type lazy susan was made in 1967 when the bearing was changed to use stellite balls, spring-type separators and stainless-steel bearing races. An extensive test program at that time showed that the design gave excellent service under all anticipated operating conditions. Fifteen of these units have been installed in the past ten years and have been essentially trouble-free. Although the bearing design for the Mark III was very similar, the component layout was such that irradiation-induced heating with associated thermal expansion resulted in decreased bearing clearance and an increase in the required driving torque. The solution involved redesign and re-arrangement of the rack drive mechanism. A series of stringent operational proof tests were made under high temperature and temperature differential conditions which proved successful operation of the new design. The severe conditions under which these tests were performed uncovered further difficulties with the bearing and led to a re-evaluation of the bearing design. A new design was developed in which the spring separators were replaced by similar sized, cylindrical graphite spacers. The entire series of operational and life tests were repeated and the performance was outstanding. Acceptable wear characteristics of the spacers were verified and the bearing was noticeably smoother and quieter than with previous designs. A Mark III lazy susan of this new design was installed in a TRIGA about one year ago and operated at power levels up to 2 MW with excellent performance. The Mark II design has now been changed to incorporate the new drive and bearing design proven for the Mark III. (author)

  6. Seismic behavior of steel storage pallet racking systems

    CERN Document Server

    Castiglioni, Carlo Andrea

    2016-01-01

    This book presents the main outcomes of the first European research project on the seismic behavior of adjustable steel storage pallet racking systems. In particular, it describes a comprehensive and unique set of full-scale tests designed to assess such behavior. The tests performed include cyclic tests of full-scale rack components, namely beam-to-upright connections and column base connections; static and dynamic tests to assess the friction factor between pallets and rack beams; full-scale pushover and pseudodynamic tests of storage racks in down-aisle and cross-aisle directions; and full-scale dynamic tests on two-bay, three-level rack models. The implications of the findings of this extensive testing regime on the seismic behavior of racking systems are discussed in detail, highlighting e.g. the confirmation that under severe dynamic conditions “sliding” is the main factor influencing rack response. This work was conceived during the development of the SEISRACKS project. Its outcomes will contribute...

  7. Fabrication of the Spent Fuel Elements Rack on the ISFSF

    International Nuclear Information System (INIS)

    Slamet Wiranto; Sigit Purwanto; Safrul, H.

    2004-01-01

    The Interim Storage For Spent Fuel elements (ISFSF) was designed to be able to store the 33 spent fuel element racks with capacity of 1386 of normal spent fuel elements and 2 racks for 36 of defected ones. Until now, only 9 out of 33 racks of normal spent fuel elements and lout of 2 racks of defected fuel elements are available. Five of them have suffered from corrosion so that they are not fulfilled the requirements of the spent fuel elements storage anymore. Meanwhile, the spent fuel storage racks in the reactor are almost full. It means, the transfer of the spent fuel from reactor spent fuel storage to the ISFSF pool are compulsory needed. Therefore, it is necessary to provide the new ISFSF spent fuel storage rack with better material and fabrication method than the old one. In this design all materials consist of SS 316 L that are welded with the Argon TIG-welding. Right now there has been one new spent fuel storage rack fabricated with capacity of 42 normal spent fuel elements. (author)

  8. Active vibration isolation platform on base of magnetorheological elastomers

    Energy Technology Data Exchange (ETDEWEB)

    Mikhailov, Valery P., E-mail: mikhailov@bmstu.ru; Bazinenkov, Alexey M.

    2017-06-01

    The article describes the active vibration isolation platform on base of magnetorheological (MR) elastomers. An active damper based on the MR elastomers can be used as an actuator of micro- or nanopositioning for a vibroinsulated object. The MR elastomers give such advantages for active control of vibration as large range of displacements (up to 1 mm), more efficient absorption of the vibration energy, possibility of active control of amplitude-frequency characteristics and positioning with millisecond response speed and nanometer running accuracy. The article presents the results of experimental studies of the most important active damper parameters. Those are starting current, transient time for stepping, transmission coefficient of the vibration displacement amplitude.

  9. Increased dopaminergic activity in socially isolated rats: an electrophysiological study

    DEFF Research Database (Denmark)

    Fabricius, Katrine; Helboe, Lone; Fink-Jensen, Anders

    2010-01-01

    The development of animal models mimicking symptoms associated with schizophrenia has been a critical step in understanding the neurobiological mechanisms underlying the disease. Long-term social isolation from weaning in rodents, a model based on the neurodevelopmental hypothesis of schizophrenia......, has been suggested to mimic some of the deficits seen in schizophrenic patients. We confirm in the present study that socially isolated rats display an increase in both spontaneous and d-amphetamine-induced locomotor activity, as well as deficits in sensorimotor gating as assessed in a pre......, and a change of firing activity towards a more irregular and bursting firing pattern. Taken together, our findings suggest that the behavioral phenotype induced by social isolation may be driven by an overactive dopamine system....

  10. Decreased Blastocyst Production in Mice Exposed to Increased Rack Noise

    Science.gov (United States)

    Zamora, Bernadette M; Jiang, Meisheng; Wang, Ying; Chai, Minghua; Lawson, P Timothy; Lawson, Gregory W

    2009-01-01

    This study was conducted to investigate the possible effect of rack type on the blastocyst yield of mouse embryo donors. The first phase of the study consisted of housing some mice (group A) in a ventilated rack and others (group B) in a static rack in the same room for 3 d, followed by euthanasia for blastocyst collection and corticosterone assay. Parametric tests were used to compare groups. The number of blastocysts per donor was lower in group A (5.0 ± 1.4 blastocysts) than group B (13.1 ± 3.7 blastocysts). Mean noise was higher in the ventilated rack (80.4 dBC) than in the static rack (69.2 dBC). Serum corticosterone concentrations did not differ between groups. For the second phase of the study, a third group of mice (group C) was housed in a static rack without a ventilated rack in the same room. The noise level for group C was even lower (45.18 ± 2.91 dBC), and the blastocyst count per donor (16.4 ± 2.4) was higher than that of group B. The mean noise levels of empty ventilated and static racks differed significantly between groups for 10 different sound frequencies. Plotting mean blastocyst production against mean rack noise revealed a negative linear relationship with good strength of correlation. These results support the earlier observation that decreased blastocyst count occurs following housing of bred C57BL/6 donor mice in ventilated cages. PMID:19807968

  11. Assessment of larvicidal activities of bacillus species isolated from ...

    African Journals Online (AJOL)

    Assessment of larvicidal activities of bacillus species isolated from soil against the mosquito aedes aegyptia (diptera: culicidae) in Sokoto, northwestern Nigeria. S.B. Manga, A.H. Kawo, A.B. Rabah, A.A. Usman, A.I. Dabai, J.A. Bala ...

  12. Antibacterial activity of chrysophanol isolated from Aloe excelsa ...

    African Journals Online (AJOL)

    Extraction of the yellow colour compounds of leaves of Aloe excelsa were performed and 1,8-dihydroxy-3-methylanthracenedione (chrysophanol) was isolated and tested for antibacterial activities against four gram negative and five gram positive bacterial strains. The structures of chrysophanol was determined by chemical ...

  13. Detection of efflux pump activity among clinical isolates of ...

    African Journals Online (AJOL)

    Purpose: To detect efflux pump activity (EPA) and screening a suspected efflux pump inhibitor (EPI) [1- (3-(trifluoromethyl)benzyl]-piperazine (TFMBP)], which could help in reducing multi-drug resistance (MDR). Methods: Eighteen isolates, viz, 14 S. aureus, 2 S. lentus, 1 S. xylosus and 1 Micrococcus species from various ...

  14. Antileishmanial activity of piceatannol isolated from Euphorbia lagascae seeds

    NARCIS (Netherlands)

    Duarte, Noelia; Kayser, Oliver; Abreu, Pedro; Ferreira, Maria-Jose U.

    In the search for biologically active compounds from Euphorbia lagascae Spreng, an herbaceous plant native to southeast of Iberic Peninsula, a stilbene, two coumarins and two 1-2-deoxyphorbol diterpene esters were isolated by chromatographic methods, from the methanol extracts of its defatted seeds.

  15. A novel acyclic diterpenic alcohol isolated from antioxidant active ...

    African Journals Online (AJOL)

    Background: Isolation and characterization of a new compound from the antioxidant active ethanol extract of leaves of an endemic plant Centaurothamnus maximus. Methods: The air dried powdered leaves of the plant was extracted successively with n-hexane, dichloromethane, ethyl acetate and ethanol. The obtained ...

  16. Experiments on active precision isolation with a smart conical adapter

    Science.gov (United States)

    Li, H.; Li, H. Y.; Chen, Z. B.; Tzou, H. S.

    2016-07-01

    Based on a conical shell adaptor, an active vibration isolator for vibration control of precision payload is designed and tested in this study. Flexible piezoelectric sensors and actuators are bonded on the adaptor surface for active vibration monitoring and control. The mathematical model of a piezoelectric laminated conical shell is derived and then optimal design of the actuators is performed for the first axial vibration mode of the isolation system. A scaled conical adaptor is manufactured with four MFC actuators laminating on its outer surface. Active vibration isolation efficiency is then evaluated on a vibration shaker. The control model is built in Matlab/Simulink and programmed into the dSPACE control board. Experimental results show that, the proposed active isolator is effective in vibration suppression of payloads with the negative velocity feedback control. In contrast, the amplitude responses increase with positive feedback control. Furthermore, the amplitude responses increases when time delay is added into the control signals, and gets the maximum when the delay is close to one quarter of one cycle time.

  17. antibacterial activity of endophytic fungi isolated from conifers needles

    African Journals Online (AJOL)

    Ravnikar, Matjaž

    2015-03-11

    Mar 11, 2015 ... taxonomically place fungi producing ones to determined active metabolites. Seventy three strains of endophytic fungi were isolated ... great number of diverse bioactive compounds (Devaraju and Satish, 2010), which have been ... closed with a glass stopper. The extraction solvents utilized were methanol ...

  18. Anti-Inflammatory Activity of Compounds Isolated from Plants

    Directory of Open Access Journals (Sweden)

    R.M. Perez G.

    2001-01-01

    Full Text Available This review shows over 300 compounds isolated and identified from plants that previously demonstrated anti-inflammatory activity. They have been classified in appropriate chemical groups and data are reported on their pharmacological effects, mechanisms of action, and other properties.

  19. Behavior of high-density spent-fuel storage racks

    International Nuclear Information System (INIS)

    Bailey, W.J.

    1986-08-01

    Included in this report is a summary of information on neutron-absorbing materials such as B 4 C in an aluminum matrix or organic binder material, stainless steel-boron and aluminum-boron alloys, and stainless steetl-clad cadmium that are used in high-density spent fuel storage racks. A list of the types of neutron-absorbing materials being used in spent fuel storage racks at domestic commercial plants is provided. Recent cases at several domestic plants where swelling of rack side plates (where the B 4 C in an aluminum matrix and B 4 C in an organic binder material were located) occurred are reviewed

  20. Laboratory racks are installed in the MPLM Leonardo

    Science.gov (United States)

    2000-01-01

    In the Space Station Processing Facility, another laboratory rack is placed on the arm of the Rack Insertion Unit to lift it to the workstand height of the Multi-Purpose Logistics Module Leonardo (not seen). The MPLM will transport laboratory racks filled with equipment, experiments and supplies to and from the International Space Station aboard the Space Shuttle. Leonardo will be launched for the first time March 1, 2001, on Shuttle mission STS-102. On that flight, Leonardo will be filled with equipment and supplies to outfit the U.S. laboratory module, being carried to the ISS on the Jan. 19, 2001, launch of STS-98.

  1. 76 FR 62364 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Preliminary...

    Science.gov (United States)

    2011-10-07

    ... Shelving and Racks From the People's Republic of China: Preliminary Results of the Countervailing Duty... countervailable duty order on certain kitchen appliance shelving and racks (``Kitchen Racks'') from the People's...: Background On July 27, 2009, the Department published a CVD order on Kitchen Racks from the PRC. See Certain...

  2. 78 FR 63166 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Countervailing...

    Science.gov (United States)

    2013-10-23

    ... Shelving and Racks From the People's Republic of China: Countervailing Duty Administrative Review; 2011... order on certain kitchen appliance shelving and racks (kitchen racks) from the People's Republic of.... Scope of the Order This order covers shelving and racks for refrigerators, freezers, combined...

  3. 77 FR 61396 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Countervailing...

    Science.gov (United States)

    2012-10-09

    ... Shelving and Racks From the People's Republic of China: Countervailing Duty Administrative Review; 2010... order on certain kitchen appliance shelving and racks (``kitchen racks'') from the People's Republic of...-1785, respectively. Scope of the Order The scope of the order consists of shelving and racks for...

  4. Nuclear import of transcription factor BR-C is mediated by its interaction with RACK1.

    Science.gov (United States)

    Cheng, Daojun; Qian, Wenliang; Wang, Yonghu; Meng, Meng; Wei, Ling; Li, Zhiqing; Kang, Lixia; Peng, Jian; Xia, Qingyou

    2014-01-01

    The transcription factor Broad Complex (BR-C) is an early ecdysone response gene in insects and contains two types of domains: two zinc finger domains for the activation of gene transcription and a Bric-a-brac/Tramtrack/Broad complex (BTB) domain for protein-protein interaction. Although the mechanism of zinc finger-mediated gene transcription is well studied, the partners interacting with the BTB domain of BR-C has not been elucidated until now. Here, we performed a yeast two-hybrid screen using the BTB domain of silkworm BR-C as bait and identified the receptor for activated C-kinase 1 (RACK1), a scaffolding/anchoring protein, as the novel partner capable of interacting with BR-C. The interaction between BR-C and RACK1 was further confirmed by far-western blotting and pull-down assays. Importantly, the disruption of this interaction, via RNAi against the endogenous RACK1 gene or deletion of the BTB domain, abolished the nuclear import of BR-C in BmN4 cells. In addition, RNAi against the endogenous PKC gene as well as phosphorylation-deficient mutation of the predicted PKC phosphorylation sites at either Ser373 or Thr406 in BR-C phenocopied RACK1 RNAi and altered the nuclear localization of BR-C. However, when BTB domain was deleted, phosphorylation mimics of either Ser373 or Thr406 had no effect on the nuclear import of BR-C. Moreover, mutating the PKC phosphorylation sites at Ser373 and Thr406 or deleting the BTB domain significantly decreased the transcriptional activation of a BR-C target gene. Given that RACK1 is necessary for recruiting PKC to close and phosphorylate target proteins, we suggest that the PKC-mediated phosphorylation and nuclear import of BR-C is determined by its interaction with RACK1. This novel finding will be helpful for further deciphering the mechanism underlying the role of BR-C proteins during insect development.

  5. Nuclear import of transcription factor BR-C is mediated by its interaction with RACK1.

    Directory of Open Access Journals (Sweden)

    Daojun Cheng

    Full Text Available The transcription factor Broad Complex (BR-C is an early ecdysone response gene in insects and contains two types of domains: two zinc finger domains for the activation of gene transcription and a Bric-a-brac/Tramtrack/Broad complex (BTB domain for protein-protein interaction. Although the mechanism of zinc finger-mediated gene transcription is well studied, the partners interacting with the BTB domain of BR-C has not been elucidated until now. Here, we performed a yeast two-hybrid screen using the BTB domain of silkworm BR-C as bait and identified the receptor for activated C-kinase 1 (RACK1, a scaffolding/anchoring protein, as the novel partner capable of interacting with BR-C. The interaction between BR-C and RACK1 was further confirmed by far-western blotting and pull-down assays. Importantly, the disruption of this interaction, via RNAi against the endogenous RACK1 gene or deletion of the BTB domain, abolished the nuclear import of BR-C in BmN4 cells. In addition, RNAi against the endogenous PKC gene as well as phosphorylation-deficient mutation of the predicted PKC phosphorylation sites at either Ser373 or Thr406 in BR-C phenocopied RACK1 RNAi and altered the nuclear localization of BR-C. However, when BTB domain was deleted, phosphorylation mimics of either Ser373 or Thr406 had no effect on the nuclear import of BR-C. Moreover, mutating the PKC phosphorylation sites at Ser373 and Thr406 or deleting the BTB domain significantly decreased the transcriptional activation of a BR-C target gene. Given that RACK1 is necessary for recruiting PKC to close and phosphorylate target proteins, we suggest that the PKC-mediated phosphorylation and nuclear import of BR-C is determined by its interaction with RACK1. This novel finding will be helpful for further deciphering the mechanism underlying the role of BR-C proteins during insect development.

  6. Screening on Gibberellic Acid Producing Activity of Azospirillum Isolates

    International Nuclear Information System (INIS)

    Shun Lai Ei; Khin Mya Lwin; Myo Myint

    2010-12-01

    Six strains of Azopirillum spp were isolated from rice, sugarcane, corn, maize, sunflower and pepper roots and screened the gibberellic acid productivity. Only three strains of Azospirillum species showed the activity and were indentified by cultural, biochemical and drug sensitivity patterns. Among them,one strain isolated from rice root can produce microbial gibberellic acid. It showed greenish yellow colour in chromatogram under UV absorption. This screening method was studied from 1 to 14 days incubation. Qualitative measurement of GA productivity was determined by thin layer chromatography.

  7. Active Fault Detection and Isolation for Hybrid Systems

    DEFF Research Database (Denmark)

    Gholami, Mehdi; Schiøler, Henrik; Bak, Thomas

    2009-01-01

    An algorithm for active fault detection and isolation is proposed. In order to observe the failure hidden due to the normal operation of the controllers or the systems, an optimization problem based on minimization of test signal is used. The optimization based method imposes the normal and faulty...... models predicted outputs such that their discrepancies are observable by passive fault diagnosis technique. Isolation of different faults is done by implementation a bank of Extended Kalman Filter (EKF) where the convergence criterion for EKF is confirmed by Genetic Algorithm (GA). The method is applied...

  8. Materials Science Research Rack Onboard the International Space Station

    Science.gov (United States)

    Reagan, Shawn; Frazier, Natalie; Lehman, John

    2016-01-01

    The Materials Science Research Rack (MSRR) is a research facility developed under a cooperative research agreement between NASA and ESA for materials science investigations on the International Space Station (ISS). MSRR was launched on STS-128 in August 2009 and currently resides in the U.S. Destiny Laboratory Module. Since that time, MSRR has logged more than 1400 hours of operating time. The MSRR accommodates advanced investigations in the microgravity environment on the ISS for basic materials science research in areas such as solidification of metals and alloys. The purpose is to advance the scientific understanding of materials processing as affected by microgravity and to gain insight into the physical behavior of materials processing. MSRR allows for the study of a variety of materials, including metals, ceramics, semiconductor crystals, and glasses. Materials science research benefits from the microgravity environment of space, where the researcher can better isolate chemical and thermal properties of materials from the effects of gravity. With this knowledge, reliable predictions can be made about the conditions required on Earth to achieve improved materials. MSRR is a highly automated facility with a modular design capable of supporting multiple types of investigations. The NASA-provided Rack Support Subsystem provides services (power, thermal control, vacuum access, and command and data handling) to the ESA-developed Materials Science Laboratory (MSL) that accommodates interchangeable Furnace Inserts (FI). Two ESA-developed FIs are presently available on the ISS: the Low Gradient Furnace (LGF) and the Solidification and Quenching Furnace (SQF). Sample Cartridge Assemblies (SCAs), each containing one or more material samples, are installed in the FI by the crew and can be processed at temperatures up to 1400?C. ESA continues to develop samples with 14 planned for launch and processing in the near future. Additionally NASA has begun developing SCAs to

  9. Antibacterial activity of chemical constituents isolated from Asparagus racemosus

    Directory of Open Access Journals (Sweden)

    Muhammad Abdullah Shah

    2014-03-01

    Full Text Available Asparagus racemosus is a medical extensively used in traditional medicine for various disorders including its use in infectious. So far work has been done to identify its active constituents responsible for antiseptic folk use of this plant. In the current investigation, we have made an effort to identify its chemical constituents that might be partly responsible for antimicrobial properties. Extraction and isolation of plant extract lead to isolation of two nor-lignans and two steroidal triterpenes (compound 1 to 4. All compound showed considerable antibacterial activities against E. coli and S. aureus while no significant activity was observed against S. typhi. This study highlighted the potential of A. racemosus to be further explored as a source of bioactive natural products.

  10. The Control of Transmitted Power in an Active Isolation System

    DEFF Research Database (Denmark)

    Elliott, S.J.; Gardonio, P.; Pinnington, R.J.

    1997-01-01

    The isolation of vibration through a system with multiple active mounts is discussed, in which each of the mounts can transmit vibration in several degrees of freedom. Theoretical models of the various parts of this system have been developed which include a flexible receiving structure and distr......The isolation of vibration through a system with multiple active mounts is discussed, in which each of the mounts can transmit vibration in several degrees of freedom. Theoretical models of the various parts of this system have been developed which include a flexible receiving structure...... and distributed active mounts, and these models can be connected together to produce an overall theoretical description of a realistic active isolation system. Total transmitted power has been found to be an excellent criterion to quantify the effect of various control strategies in this model in which...... the contributions to the transmitted power in the various degrees of freedom can be clearly understood. It has also been found, however, that an active control system which minimises a practical estimate of transmitted power, calculated from the product of the axial forces and velocities under the mounts, can give...

  11. Video Monitoring and Analysis System for Vivarium Cage Racks | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    This invention pertains to a system for continuous observation of rodents in home-cage environments with the specific aim to facilitate the quantification of activity levels and behavioral patterns for mice housed in a commercial ventilated cage rack.  The National Cancer Institute’s Radiation Biology Branch seeks partners interested in collaborative research to co-develop a video monitoring system for laboratory animals.

  12. Rack Distribution Effects on MPLM Center of Mass

    Science.gov (United States)

    Tester, John T.

    2005-01-01

    This research was in support of exploring the need for more flexible "center of gravity (CG) specifications than those currently established by NASA for the Multi-Purpose Logistics Module (MPLM). The MPLM is the cargo carrier for International Space Station (ISS) missions. The MPLM provides locations for 16 standard racks, as shown in Figure 1; not all positions need to be filled in any given flight. The MPLM coordinate system (X(sub M), Y(sub M), Z(sub M)) is illustrated as well. For this project, the primary missions of interest were those which supply the ISS and remove excess materials on the return flights. These flights use a predominate number of "Resupply Stowage Racks" (RSR) and "Resupply Stowage Platforms" (RSP). In these two types of racks, various smaller items are stowed. Hence, these racks will exhibit a considerable range of mass values as well as a range as to where their individual CG are located.

  13. Retort Racks for Polymeric Trays in 1400 Style Spray Retorts

    National Research Council Canada - National Science Library

    Bruins, Henderikus B

    2003-01-01

    The objective of this project was to design a retort rack that would maximize the retort capacity of a 1400 style spray retort and to select a material that would withstand the harsh retort spray environment...

  14. Criticality safety analysis of the NPP Krsko storage racks

    International Nuclear Information System (INIS)

    Kromar, M.; Kurincic, B.

    2002-01-01

    NPP Krsko is going to increase the capacity of the spent fuel storage pool by replacement of the existing racks with high-density racks. This will be the second reracking campaign since 1983 when storage was increased from 180 to 828 storage locations. The pool capacity will increase from 828 to 1694 with partial reracking by the spring 2003. The installed capacity will be sufficient for the current design plant lifetime. Complete reracking of the spent fuel pool will additionally increase capacity to 2321 storage locations. The design, rack manufacturing and installation has been awarded to the Framatome ANP GmbH. Burnup credit methodology, which was approved by the Slovenian Nuclear Safety Administration in previous licensing of existing racks, will be again implemented in the licensing process with the recent methodology improvements. Specific steps of the criticality safety analysis and representative results are presented in the paper.(author)

  15. Seismic and structural analysis of high density/consolidated spent fuel storage racks

    International Nuclear Information System (INIS)

    Shah, S.J.; Biddle, J.R.; Bennett, S.M.; Schechter, C.B.; Harstead, G.A.; Kopecky, B.

    1995-01-01

    In many nuclear power plants, existing storage racks are being replaced with high-density racks to accommodate the increasing inventory of spent fuel. In the hypothetical design considered here, the high-density arrangement of fuel assemblies, or consolidated fuel canisters, is accomplished through the use of borated stainless steel (BSS) plates acting as neutron absorbers. The high-density fuel racks are simply supported by the pool floor with no structural connections to adjacent racks or to the pool walls or floor. Therefore, the racks are free standing and may slide and tip. Several time history, nonlinear, seismic analyses are required to account for variations in the coefficient of friction, rack loading configuration, ad the type of the seismic event. This paper presents several of the mathematical models usually used. The models include features to allow sliding and tipping of the racks and to represent the hydrodynamic coupling which can occur between fuel assemblies and rack cells, between adjacent racks, and between the racks and the reinforced concrete walls. A detailed model representing a single rack is used to evaluate the 3-D loading effects. This model is a controlling case for the stress analysis. A 2-D multi-rack model representing a row of racks between the spent fuel pool walls is used to evaluate the change in gaps between racks. The racks are analyzed for the fuel loading conditions of consolidated, full, empty, and half-loaded with fuel assemblies

  16. Multiresistant Bacteria Isolated from Activated Sludge in Austria

    Directory of Open Access Journals (Sweden)

    Herbert Galler

    2018-03-01

    Full Text Available Wastewater contains different kinds of contaminants, including antibiotics and bacterial isolates with human-generated antibiotic resistances. In industrialized countries most of the wastewater is processed in wastewater treatment plants which do not only include commercial wastewater, but also wastewater from hospitals. Three multiresistant pathogens—extended spectrum β-lactamase (ESBL-harbouring Enterobacteriaceae (Gram negative bacilli, methicillin resistant Staphylococcus aureus (MRSA and vancomycin resistant Enterococci (VRE—were chosen for screening in a state of the art wastewater treatment plant in Austria. Over an investigation period of six months all three multiresistant pathogens could be isolated from activated sludge. ESBL was the most common resistance mechanism, which was found in different species of Enterobacteriaceae, and in one Aeromonas spp. Sequencing of ESBL genes revealed the dominance of genes encoding members of CTX-M β-lactamases family and a gene encoding for PER-1 ESBL was detected for the first time in Austria. MRSA and VRE could be isolated sporadically, including one EMRSA-15 isolate. Whereas ESBL is well documented as a surface water contaminant, reports of MRSA and VRE are rare. The results of this study show that these three multiresistant phenotypes were present in activated sludge, as well as species and genes which were not reported before in the region. The ESBL-harbouring Gram negative bacilli were most common.

  17. Multiresistant Bacteria Isolated from Activated Sludge in Austria

    Science.gov (United States)

    Feierl, Gebhard; Petternel, Christian; Reinthaler, Franz F.; Haas, Doris; Habib, Juliana; Kittinger, Clemens; Luxner, Josefa

    2018-01-01

    Wastewater contains different kinds of contaminants, including antibiotics and bacterial isolates with human-generated antibiotic resistances. In industrialized countries most of the wastewater is processed in wastewater treatment plants which do not only include commercial wastewater, but also wastewater from hospitals. Three multiresistant pathogens—extended spectrum β-lactamase (ESBL)-harbouring Enterobacteriaceae (Gram negative bacilli), methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Enterococci (VRE)—were chosen for screening in a state of the art wastewater treatment plant in Austria. Over an investigation period of six months all three multiresistant pathogens could be isolated from activated sludge. ESBL was the most common resistance mechanism, which was found in different species of Enterobacteriaceae, and in one Aeromonas spp. Sequencing of ESBL genes revealed the dominance of genes encoding members of CTX-M β-lactamases family and a gene encoding for PER-1 ESBL was detected for the first time in Austria. MRSA and VRE could be isolated sporadically, including one EMRSA-15 isolate. Whereas ESBL is well documented as a surface water contaminant, reports of MRSA and VRE are rare. The results of this study show that these three multiresistant phenotypes were present in activated sludge, as well as species and genes which were not reported before in the region. The ESBL-harbouring Gram negative bacilli were most common. PMID:29522474

  18. Azoreductase activity of anaerobic bacteria isolated from human intestinal microflora.

    Science.gov (United States)

    Rafii, F; Franklin, W; Cerniglia, C E

    1990-01-01

    A plate assay was developed for the detection of anaerobic bacteria that produce azoreductases. With this plate assay, 10 strains of anaerobic bacteria capable of reducing azo dyes were isolated from human feces and identified as Eubacterium hadrum (2 strains), Eubacterium spp. (2 species), Clostridium clostridiiforme, a Butyrivibrio sp., a Bacteroides sp., Clostridium paraputrificum, Clostridium nexile, and a Clostridium sp. The average rate of reduction of Direct Blue 15 dye (a dimethoxybenzidine-based dye) in these strains ranged from 16 to 135 nmol of dye per min per mg of protein. The enzymes were inactivated by oxygen. In seven isolates, a flavin compound (riboflavin, flavin adenine dinucleotide, or flavin mononucleotide) was required for azoreductase activity. In the other three isolates and in Clostridium perfringens, no added flavin was required for activity. Nondenaturing polyacrylamide gel electrophoresis showed that each bacterium expressed only one azoreductase isozyme. At least three types of azoreductase enzyme were produced by the different isolates. All of the azoreductases were produced constitutively and released extracellularly. Images PMID:2202258

  19. Azoreductase activity of anaerobic bacteria isolated from human intestinal microflora.

    Science.gov (United States)

    Rafii, F; Franklin, W; Cerniglia, C E

    1990-07-01

    A plate assay was developed for the detection of anaerobic bacteria that produce azoreductases. With this plate assay, 10 strains of anaerobic bacteria capable of reducing azo dyes were isolated from human feces and identified as Eubacterium hadrum (2 strains), Eubacterium spp. (2 species), Clostridium clostridiiforme, a Butyrivibrio sp., a Bacteroides sp., Clostridium paraputrificum, Clostridium nexile, and a Clostridium sp. The average rate of reduction of Direct Blue 15 dye (a dimethoxybenzidine-based dye) in these strains ranged from 16 to 135 nmol of dye per min per mg of protein. The enzymes were inactivated by oxygen. In seven isolates, a flavin compound (riboflavin, flavin adenine dinucleotide, or flavin mononucleotide) was required for azoreductase activity. In the other three isolates and in Clostridium perfringens, no added flavin was required for activity. Nondenaturing polyacrylamide gel electrophoresis showed that each bacterium expressed only one azoreductase isozyme. At least three types of azoreductase enzyme were produced by the different isolates. All of the azoreductases were produced constitutively and released extracellularly.

  20. Antituberculotic activity of actinobacteria isolated from the rare habitats.

    Science.gov (United States)

    Hussain, A; Rather, M A; Shah, A M; Bhat, Z S; Shah, A; Ahmad, Z; Parvaiz Hassan, Q

    2017-09-01

    A distinctive screening procedure resulted in the isolation and identification of antituberculotic actinobacteria. In this course, a total of 125 actinobacteria were isolated from various soil samples from untapped areas in Northwestern Himalayas, India. The antibacterial screening showed that 26 isolates inhibited the growth of at least one of the tested bacterial pathogens including Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (ATCC 12228), Bacillus subtilis (ATCC 11774), Micrococcus luteus (ATCC 10240), Escherichia coli (10536), Pseudomonas aeruginosa (ATCC 10145) and Klebsiella pneumonia (ATCC BAA-2146). The production media was optimized for the active strains by estimation of their extract value by the quantification of the ethyl acetate extract. The screening of fermentation products from the selected 26 bioactive isolates revealed that 10 strains have metabolites antagonistic against the standard H37Rv strain of Mycobacterium tuberculosis. The characterization by 16S rRNA gene sequencing and phylogenetic analysis demonstrated the diverse nature of these antituberculosis strains. The secondary metabolites of potent, rare strain, Lentzea violacea AS08 exhibited promising antituberculosis activity with minimal inhibitory concentration (MIC) of 3·9 μg ml -1 . The metabolites identified by gas chromatography-mass spectrometry (GC-MS) included, Phenol, 2,5-bis (1, 1-dimethylethyl), n-Hexadecanoic acid, Hexadecanoic acid methyl-ester, Hexadecanoic acid ethyl-ester and, 9,12-Octadecadienoyl chloride(Z,Z) are biologically significant molecules. The study presents the isolation of rare actinobacteria from untapped sites in the Northwestern Himalayas and their in vitro potential against Mycobacterium tuberculosis for their metabolites. The study revealed that exploring the untapped natural sources as one of the resourceful approaches for the discovery of new natural products. This study also provided strong evidence for the ability of rare and

  1. Innovative Ballasted Flat Roof Solar PV Racking System

    Energy Technology Data Exchange (ETDEWEB)

    Peek, Richard T. [Cascade Engineering, Grand Rapids, MI (United States)

    2014-12-15

    The objective of this project was to reduce the cost of racking for PV solar on flat commercial rooftops. Cost reductions would come from both labor savings and material savings related to the installation process. The rack would need to accommodate the majority of modules available on the market. Cascade Engineering has a long history of converting traditional metal type applications over to plastic. Injection molding of plastics have numerous advantages including selection of resin for the application, placing the material exactly where it is needed, designing in features that will speed up the installation process, and weight reduction of the array. A plastic rack would need to meet the requirements of UL2703, Mounting systems, mounting devices, clamping/retention devices, and ground lugs for use with flat-plate photovoltaic modules and panels. Comparing original data to the end of project racking design, racking material costs were reduced 50% and labor costs reduced 64%. The racking product accommodates all 60 and 72 cell panels on the market, meets UL2703 requirements, contributes only 1.3 pounds per square foot of weight to the array, requires little ballast to secure the array, automatically grounds the module when the module is secured, stacks/nests well for shipping/fewer lifts to the roof, provides integrated wire routing, allows water to drain on the roof, and accommodates various seismic roof connections. Project goals were achieved as noted in the original funding application.

  2. Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae).

    Science.gov (United States)

    Liang, Hanqiao; Xing, Yongmei; Chen, Juan; Zhang, Dawei; Guo, Shunxing; Wang, Chunlan

    2012-11-28

    Drug resistance in bacteria has become a global concern and the search for new antibacterial agents is urgent and ongoing. Endophytes provide an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel compounds are being isolated from endophytic fungi. Ophiopogon japonicus, containing compounds with antibacterial activity, is a traditional Chinese medicinal plant used for eliminating phlegm, relieving coughs, latent heat in the lungs, and alleviating diabetes mellitus. We investigated the antimicrobial activities of 30 strains of O. japonicus. Fungal endophytes were isolated from roots and stems of O. japonicus collected from Chongqing City, southwestern China. Mycelial extracts (MC) and fermentation broth (FB) were tested for antimicrobial activity using peptide deformylase (PDF) inhibition fluorescence assays and MTT cell proliferation assays. A total of 30 endophytic strains were isolated from O. japonicus; 22 from roots and eight from stems. 53.33% of the mycelial extracts (MC) and 33.33% of the fermentation broths (FB) displayed potent inhibition of PDF. 80% of MC and 33.33% of FB significantly inhibited Staphylococcus aureus. 70% of MC and 36.67% of FB showed strong activities against Cryptococcus neoformans. None showed influence on Escherichia coli. The secondary metabolites of endophytic fungi from O. japonicus are potential antimicrobial agents.

  3. Bioassay Guided Isolation of Active Compounds from Alchemilla barbatiflora Juz.

    Directory of Open Access Journals (Sweden)

    Gülin Renda

    2018-01-01

    Full Text Available The aerial parts of Alchemilla L. species (Rosaceae are used internally as diuretic, laxative, tonic and externally for wound healing in Turkish folk medicine. Antioxidant effects of the extracts, fractions and isolated compounds from the aerial parts of A. barbatiflora Juz. were investigated with following methods: 1,1-diphenyl-2-picryl-hydrazyl (DPPH, and superoxide radical scavenging (SOD, phosphomolibdenum-reducing antioxidant power (PRAP, ferric-reducing antioxidant power (FRAP assays. In addition, tyrosinase, α-glucosidase and acetylcholinesterase inhibition activities of samples were analyzed. The methanol extract from the aerial parts of plant was consecutively fractionated into four subextracts; n-hexane, chloroform, and remaining water extracts. Further studies were carried out on the most active water subextract and the fractions obtained from water subextract with column chromatography. Phytochemical studies on active fractions of the water subextract led to the isolation of seven metabolites including catechin (1 and a catechin dimer; procyanidin B3 (2, a flavonol glucuronide; quercetin-3-O- β-D-glucuronic acid (miquelianin (3 with flavonoid glycosides; quercetin-3-O- β-D-galactoside (hyperoside (4, quercetin-3-O- β-D-arabinoside (guaiaverin (5, kaempferol-3-O-β-D-xylopyranoside (6 and kaempferol-3-O -(6″-coumaroyl-β-D-glycoside (tiliroside (7. Their structures were elucidated by spectral techniques (1D and 2D NMR. The experimental data verified that procyanidin B3 displayed remarkable enzyme inhibitory activity among the whole isolated compounds.

  4. RACK1 forms a complex with FGFR1 and PKM2, and stimulates the growth and migration of squamous lung cancer cells.

    Science.gov (United States)

    Zhou, Chengzhi; Chen, Tao; Xie, Zhanhong; Qin, Yinyin; Ou, Yangming; Zhang, Jiexia; Li, Shiyue; Chen, Rongchang; Zhong, Nanshan

    2017-11-01

    Phosphorylation of Pyruvate Kinase M2 (PKM2) on Tyr105 by fibroblast growth factor receptor 1 (FGFR1) has been shown to promote its nuclear localization as well as cell growth in lung cancer. Better understanding the regulation of this process would benefit the clinical treatment for lung cancer. Here, it has been found that the adaptor protein receptor for activated PKC kinase (RACK1) formed a complex with FGFR1 and PKM2, and activated the FGFR1/PKM2 signaling. Knocking down the expression of RACK1 impaired the phosphorylation on Tyr105 of PKM2 and inhibited the growth and migration of lung cancer cells, while over-expression of RACK1 in lung cancer cells led to the resistance to Erdafitinib. Moreover, knocking down the expression of RACK1 impaired the tumorigenesis of lung cancer driven by LKB loss and mutated Ras (KrasG12D). Taken together, our study demonstrated the pivotal roles of RACK1 in FGFR1/PKM2 signaling, suggesting FGFR1/RACK1/PKM2 might be a therapeutic target for lung cancer treatment. © 2017 Wiley Periodicals, Inc.

  5. Characterization of newly isolated lytic bacteriophages active against Acinetobacter baumannii.

    Directory of Open Access Journals (Sweden)

    Maia Merabishvili

    Full Text Available Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB_AbaM_Acibel004 and podovirus vB_AbaP_Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively, high burst size (125 and 145, respectively, stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.

  6. Isolation of allergenically active glycoprotein from Prosopis juliflora pollen.

    Science.gov (United States)

    Thakur, I S

    1989-03-01

    An allergenically active glycoprotein was homogeneously isolated from the aqueous extract of Prosopis juliflora pollen by ConA-Sepharose affinity chromatography. The molecular weight of this glycoprotein was 20,000 dalton, determined by gel filtration and SDS-PAGE. This fraction showed a total carbohydrate concentration of 25%. The purified glycoprotein revealed immunochemically most antigenic or allergenic and demonstrated homogeneous after reaction with P. juliflora pollen antiserum, characterized by gel diffusion, Immunoelectrophoresis and Radioallergosorbent test.

  7. In vitro activity of ivermectin against Staphylococcus aureus clinical isolates

    Directory of Open Access Journals (Sweden)

    Shoaib Ashraf

    2018-02-01

    Full Text Available Abstract Background Ivermectin is an endectocide against many parasites. Though being a macrocyclic lactone, its activity against bacteria has been less known, possibly due to the fact that micromolar concentrations at tissue levels are required to achieve a therapeutic effect. Among pathogenic bacteria of major medical significance, Staphylococcus aureus cause a number of diseases in a wide variety of hosts including humans and animals. It has been attributed as one of the most pathogenic organisms. The emergence of methicillin resistance has made the treatment of S. aureus even more difficult as it is now resistant to most of the available antibiotics. Thus, search for alternate anti-staphylococcal agents requires immediate attention. Methods Twenty-one clinical isolates of S. aureus were isolated from bovine milk collected from Lahore and Faisalabad Pakistan. Different anthelmintics including levamisole, albendazole and ivermectin were tested against S. aureus to determine their minimum inhibitory concentrations. This was followed-up by growth curve analysis, spot assay and time-kill kinetics. Results The results showed that ivermectin but not levamisole or albendazole exhibited a potent anti-staphylococcal activity at the concentrations of 6.25 and 12.5 μg/ml against two isolates. Interestingly, one of the isolate was sensitive while the other was resistant to methicillin/cefoxitin. Conclusions Our novel findings indicate that ivermectin has an anti-bacterial effect against certain S. aureus isolates. However, to comprehend why ivermectin did not inhibit the growth of all Staphylococci needs further investigation. Nevertheless, we have extended the broad range of known pharmacological effects of ivermectin. As pharmacology and toxicology of ivermectin are well known, its further development as an anti-staphylococcal agent is potentially appealing.

  8. Nonlinear damping based semi-active building isolation system

    Science.gov (United States)

    Ho, Carmen; Zhu, Yunpeng; Lang, Zi-Qiang; Billings, Stephen A.; Kohiyama, Masayuki; Wakayama, Shizuka

    2018-06-01

    Many buildings in Japan currently have a base-isolation system with a low stiffness that is designed to shift the natural frequency of the building below the frequencies of the ground motion due to earthquakes. However, the ground motion observed during the 2011 Tohoku earthquake contained strong long-period waves that lasted for a record length of 3 min. To provide a novel and better solution against the long-period waves while maintaining the performance of the standard isolation range, the exploitation of the characteristics of nonlinear damping is proposed in this paper. This is motivated by previous studies of the authors, which have demonstrated that nonlinear damping can achieve desired performance over both low and high frequency regions and the optimal nonlinear damping force can be realized by closed loop controlled semi-active dampers. Simulation results have shown strong vibration isolation performance on a building model with identified parameters and have indicated that nonlinear damping can achieve low acceleration transmissibilities round the structural natural frequency as well as the higher ground motion frequencies that have been frequently observed during most earthquakes in Japan. In addition, physical building model based laboratory experiments are also conducted, The results demonstrate the advantages of the proposed nonlinear damping technologies over both traditional linear damping and more advanced Linear-Quadratic Gaussian (LQG) feedback control which have been used in practice to address building isolation system design and implementation problems. In comparison with the tuned-mass damper and other active control methods, the proposed solution offers a more pragmatic, low-cost, robust and effective alternative that can be readily installed into the base-isolation system of most buildings.

  9. Improved hybrid isolator with maglev actuator integrated in air spring for active-passive isolation of ship machinery vibration

    Science.gov (United States)

    Li, Yan; He, Lin; Shuai, Chang-geng; Wang, Chun-yu

    2017-10-01

    A hybrid isolator consisting of maglev actuator and air spring is proposed and developed for application in active-passive vibration isolation system of ship machinery. The dynamic characteristics of this hybrid isolator are analyzed and tested. The stability and adaptability of this hybrid isolator to shock and swing in the marine environment are improved by a compliant gap protection technique and a disengageable suspended structure. The functions of these new engineering designs are proved by analytical verification and experimental validation of the designed stiffness of such a hybrid isolator, and also by shock adaptability testing of the hybrid isolator. Finally, such hybrid isolators are installed in an engineering mounting loaded with a 200-kW ship diesel generator, and the broadband and low-frequency sinusoidal isolation performance is tested.

  10. [Isolation and antimicrobial activities of actinomycetes from vermicompost].

    Science.gov (United States)

    Wang, Xue-jun; Yan, Shuang-lin; Min, Chang-li; Yang, Yan

    2015-02-01

    In this paper, actinomycetes were isolated from vermicompost by tablet coating method. Antimicrobial activities of actinomycetes were measured by the agar block method. Strains with high activity were identified based on morphology and biochemical characteristics, as well as 16S rDNA gene sequence analysis. The results showed that 26 strains of actinomycetes were isolated, 16 of them had antimicrobial activities to the test strains which accounts for 61.54% of all strains. Among the 16 strains, the strain QYF12 and QYF22 had higher antimicrobial activity to Micrococcus luteus, with a formed inhibition zone of 27 mm and 31 mm, respectively. While the strain QYF26 had higher antimicrobial activity to Bacillus subtilis, and the inhibition zone diameter was 21 mm. Based on the identification of strains with high activity, the strain QYF12 was identified as Streptomyces chartreusis, the strain QYF22 was S. ossamyceticus and the strain QYF26 was S. gancidicus. This study provided a theoretical basis for further separate antibacterial product used for biological control.

  11. Biological Activity of Curcuminoids Isolated from Curcuma longa

    Directory of Open Access Journals (Sweden)

    Simay Çıkrıkçı

    2008-04-01

    Full Text Available Curcumin is the most important fraction of turmeric which is responsible for its biological activity. In this study, isolation and biological assessment of turmeric and curcumin have been discussed against standard bacterial and mycobacterial strains such as E.coli , S.aureus, E.feacalis, P.aeuroginosa, M.smegmatis, M.simiae, M.kansasii, M. terrae, M.szulgai and the fungi Candida albicans. The antioxidant activity of curcumin and turmeric were also determined by the CUPRAC method.

  12. Attenuated enzootic (pestoides) isolates of Yersinia pestis express active aspartase.

    Science.gov (United States)

    Bearden, Scott W; Sexton, Christopher; Pare, Joshua; Fowler, Janet M; Arvidson, Cindy G; Yerman, Lyudmyla; Viola, Ronald E; Brubaker, Robert R

    2009-01-01

    It is established that Yersinia pestis, the causative agent of bubonic plague, recently evolved from enteropathogenic Yersinia pseudotuberculosis by undergoing chromosomal degeneration while acquiring two unique plasmids that facilitate tissue invasion (pPCP) and dissemination by fleabite (pMT). Thereafter, plague bacilli spread from central Asia to sylvatic foci throughout the world. These epidemic isolates exhibit a broad host range including man as opposed to enzootic (pestoides) variants that remain in ancient reservoirs where infection is limited to muroid rodents. Cells of Y. pseudotuberculosis are known to express glucose-6-phosphate dehydrogenase (Zwf) and aspartase (AspA); these activities are not detectable in epidemic Y. pestis due to missense mutations (substitution of proline for serine at amino position 155 of Zwf and leucine for valine at position 363 of AspA). In this study, functional Zwf was found in pestoides strains E, F and G but not seven other enzootic isolates; enzymic activity was associated with retention of serine at amino acid position 155. Essentially, full AspA activity occurred in pestoides isolates where valine (pestoides A, B, C and D) or serine (pestoides E, F, G and I) occupied position 363. Reduced activity occurred in strains Angola and A16, which contained phenylalanine at this position. The kcat but not Km of purified AspA from strain Angola was significantly reduced. In this context, aspA of the recently described attenuated enzootic microtus biovar encodes active valine at position 363, further indicating that functional AspA is a biomarker for avirulence of Y. pestis in man.

  13. Recent Advances in Marine Algae Polysaccharides: Isolation, Structure, and Activities.

    Science.gov (United States)

    Xu, Shu-Ying; Huang, Xuesong; Cheong, Kit-Leong

    2017-12-13

    Marine algae have attracted a great deal of interest as excellent sources of nutrients. Polysaccharides are the main components in marine algae, hence a great deal of attention has been directed at isolation and characterization of marine algae polysaccharides because of their numerous health benefits. In this review, extraction and purification approaches and chemico-physical properties of marine algae polysaccharides (MAPs) are summarized. The biological activities, which include immunomodulatory, antitumor, antiviral, antioxidant, and hypolipidemic, are also discussed. Additionally, structure-function relationships are analyzed and summarized. MAPs' biological activities are closely correlated with their monosaccharide composition, molecular weights, linkage types, and chain conformation. In order to promote further exploitation and utilization of polysaccharides from marine algae for functional food and pharmaceutical areas, high efficiency, and low-cost polysaccharide extraction and purification methods, quality control, structure-function activity relationships, and specific mechanisms of MAPs activation need to be extensively investigated.

  14. Screening for Antiviral Activities of Isolated Compounds from Essential Oils

    Directory of Open Access Journals (Sweden)

    Akram Astani

    2011-01-01

    Full Text Available Essential oil of star anise as well as phenylpropanoids and sesquiterpenes, for example, trans-anethole, eugenol, β-eudesmol, farnesol, β-caryophyllene and β-caryophyllene oxide, which are present in many essential oils, were examined for their antiviral activity against herpes simplex virus type 1 (HSV-1 in vitro. Antiviral activity was analyzed by plaque reduction assays and mode of antiviral action was determined by addition of the drugs to uninfected cells, to the virus prior to infection or to herpesvirus-infected cells. Star anise oil reduced viral infectivity by >99%, phenylpropanoids inhibited HSV infectivity by about 60–80% and sesquiterpenes suppressed herpes virus infection by 40–98%. Both, star anise essential oil and all isolated compounds exhibited anti-HSV-1 activity by direct inactivation of free virus particles in viral suspension assays. All tested drugs interacted in a dose-dependent manner with herpesvirus particles, thereby inactivating viral infectivity. Star anise oil, rich in trans-anethole, revealed a high selectivity index of 160 against HSV, whereas among the isolated compounds only β-caryophyllene displayed a high selectivity index of 140. The presence of β-caryophyllene in many essential oils might contribute strongly to their antiviral ability. These results indicate that phenylpropanoids and sesquiterpenes present in essential oils contribute to their antiviral activity against HSV.

  15. Antagonistic Activity of Trichoderma ISolates against Sclerotium rolfsii : Screening of Efficient Isolates from Morocco Soils for Biological Control

    Directory of Open Access Journals (Sweden)

    N. Khattabi

    2004-12-01

    Full Text Available Seventy Trichoderma spp. isolates collected from different regions of Morocco were tested for their capacity to inhibit in vitro mycelial growth of Sclerotium rolfsii, and for their effect on the viability of S. rolfsii sclerotia in the soil. The Trichoderma spp. isolates inhibited mycelial growth of S. rolfsii to various degrees, with 52% of isolates expressing an average inhibition, varying between 45 and 55%. The effect on the viability of sclerotia in the soil also varied between isolates of Trichoderma, with the majority (84% having a slight effect. A group of twenty isolates identified as Trichoderma harzianum when tested in sterilized soil, significantly reduced sclerotial viability though not in natural soil. Four of these isolates (Nz, Kb2, Kb3 and Kf1 showed good antagonistic activity against S. rolfsii and were also highly competitive in natural soil. These isolates would therefore be candidates for development in biological control program.

  16. Evolution of design concepts for remotely maintainable equipment racks

    International Nuclear Information System (INIS)

    Peishel, F.L.; Mouring, R.W.; Schrock, S.L.

    1986-01-01

    Equipment racks have been used to support process equipment in radioactive facilities for many years. Improvements in the design of these racks have evolved relatively slowly primarily as a result of limitations in the capabilities of maintenance equipment; that is, tasks could only be approached from above using bridge cranes with viewing primarily through periscopes. In recent years, however, technological advances have been made by the Consolidated Fuel Reprocessing Program (CFRP) at Oak Ridge National Laboratory (ORNL) in bridge-mounted servomanipulators with onboard auxiliary hoists and television viewing systems. These advances permit full cell coverage by the manipulator arms which, in turn, allow maintenance tasks to be approached horizontally as well as from above. Maintainable equipment items can be stacked vertically on a rack because total overhead access is less important and maintenance tasks that would not have been attempted in the past can now be performed. These advances permit greater flexibility in the design and cell layout of the racks and lead to concepts that could significantly increase the availability of a facility. The evolution of rack design and a description of the alternative concepts based on present maintenance systems capabilities are presented in this paper. 13 refs., 11 figs

  17. Benchmarking criticality analysis of TRIGA fuel storage racks.

    Science.gov (United States)

    Robinson, Matthew Loren; DeBey, Timothy M; Higginbotham, Jack F

    2017-01-01

    A criticality analysis was benchmarked to sub-criticality measurements of the hexagonal fuel storage racks at the United States Geological Survey TRIGA MARK I reactor in Denver. These racks, which hold up to 19 fuel elements each, are arranged at 0.61m (2 feet) spacings around the outer edge of the reactor. A 3-dimensional model was created of the racks using MCNP5, and the model was verified experimentally by comparison to measured subcritical multiplication data collected in an approach to critical loading of two of the racks. The validated model was then used to show that in the extreme condition where the entire circumference of the pool was lined with racks loaded with used fuel the storage array is subcritical with a k value of about 0.71; well below the regulatory limit of 0.8. A model was also constructed of the rectangular 2×10 fuel storage array used in many other TRIGA reactors to validate the technique against the original TRIGA licensing sub-critical analysis performed in 1966. The fuel used in this study was standard 20% enriched (LEU) aluminum or stainless steel clad TRIGA fuel. Copyright © 2016. Published by Elsevier Ltd.

  18. Antimicrobial activities of isothiocyanates against Campylobacter jejuni isolates

    Directory of Open Access Journals (Sweden)

    Virginie eDufour

    2012-04-01

    Full Text Available Food-borne human infection with Campylobacter jejuni is a medical concern in both industrialized and developing countries. Efficient eradication of C. jejuni reservoirs within live animals and processed foods is limited by the development of antimicrobial resistances and by practical problems related to the use of conventional antibiotics in food processes.We have investigated the bacteriostatic and bactericidal activities of two phytochemicals, allyl-isothiocyanate (AITC and benzyl-isothiocyanate (BITC, against 24 C. jejuni isolates from chicken feces, human infections and contaminated foods, as well as two reference strains NCTC11168 and 81-176.Both AITC and BITC displayed a potent antibacterial activity against C. jejuni. BITC showed a higher overall antibacterial effect (MIC of 2.5 to 5 g mL-1 compared to AITC (MIC of 50 to 200 g mL-1. Interestingly, the 24 C. jejuni isolates could be classified in 3 groups according to their sensitivity levels to both compounds, suggesting that AITC and BITC shared identical activity mechanisms and consequently faced similar resistance processes in bacterial cells.The sensitivity levels of C. jejuni strains against isothiocyanates were neither correlated with the presence of a GGT (-Glutamyl Transpeptidase encoding gene in the genome nor with the origin of the biological sample. However the ggt mutant of C. jejuni 81-176 displayed a decreased survival rate compared to WT when exposed to ITC.

  19. Nonlinear dynamic response of whole pool multiple spent fuel racks subject to three-dimensional excitations

    International Nuclear Information System (INIS)

    Zhao, Y.; Wilson, P.R.; Stevenson, J.D.

    1995-01-01

    The seismic evaluation of submerged free standing spent fuel storage racks is more complicated than most other nuclear structural systems. When subjected to three dimensional (3-D) floor seismic excitations the dynamic responses of racks in a pool are hydro dynamically coupled with each other, with the fuel assemblies water in gaps. The motion behavior of the racks is significantly different from that observed using a 3D single rack mode. Few seismic analyses using 3-D whole pool multiple rack models are available in the literature. I this paper an analysis was performed for twelve racks using potential theory for the fluid-structure interaction, and using a 3-D whole pool multi-rack finite element model developed herein. The analysis includes the potential nonlinear dynamic behavior of the impact of fuel-rack, rack-rack and rack-pool wall, the tilting or uplift and the frictional sliding of rack supports, and the impact of the rack supports to the pool floor. (author). 12 refs., 7 figs., 1 tab

  20. Microgravity Active Vibration Isolation System on Parabolic Flights

    Science.gov (United States)

    Dong, Wenbo; Pletser, Vladimir; Yang, Yang

    2016-07-01

    The Microgravity Active Vibration Isolation System (MAIS) aims at reducing on-orbit vibrations, providing a better controlled lower gravity environment for microgravity physical science experiments. The MAIS will be launched on Tianzhou-1, the first cargo ship of the China Manned Space Program. The principle of the MAIS is to suspend with electro-magnetic actuators a scientific payload, isolating it from the vibrating stator. The MAIS's vibration isolation capability is frequency-dependent and a decrease of vibration of about 40dB can be attained. The MAIS can accommodate 20kg of scientific payload or sample unit, and provide 30W of power and 1Mbps of data transmission. The MAIS is developed to support microgravity scientific experiments on manned platforms in low earth orbit, in order to meet the scientific requirements for fluid physics, materials science, and fundamental physics investigations, which usually need a very quiet environment, increasing their chances of success and their scientific outcomes. The results of scientific experiments and technology tests obtained with the MAIS will be used to improve future space based research. As the suspension force acting on the payload is very small, the MAIS can only be operative and tested in a weightless environment. The 'Deutsches Zentrum für Luft- und Raumfahrt e.V.' (DLR, German Aerospace Centre) granted a flight opportunity to the MAIS experiment to be tested during its 27th parabolic flight campaign of September 2015 performed on the A310 ZERO-G aircraft managed by the French company Novespace, a subsidiary of the 'Centre National d'Etudes Spatiales' (CNES, French Space Agency). The experiment results confirmed that the 6 degrees of freedom motion control technique was effective, and that the vibration isolation performance fulfilled perfectly the expectations based on theoretical analyses and simulations. This paper will present the design of the MAIS and the experiment results obtained during the

  1. A new electronic circuit for NIM rack power supply

    International Nuclear Information System (INIS)

    Toledo, Fabio de; Domienikan, Claudio; Zahn, Guilherme S.

    2013-01-01

    A new and useful electronic circuit designed to substitute the old power supply of IPEN model PM 1212 Nuclear Instrument Modules (NIM) racks is presented. Originally, these NIM racks were projected by IPEN workshop at 1974 to be used in nuclear research laboratories. The PM1212 provided mounting space and power sources for up to 12 standard nuclear electronic modules and power supply regulated DC voltages of ±24V (2A), ±12V (4A) and unregulated AC 117V (0.5A). Currently, several of these NIM racks are not in use because the electronic components of the original design have become obsolete and are no longer manufactured. The new electronic circuit provides the same original voltages adjusted in the single circuit and, additionally, ±6V. The new power supply was designed and constructed at IPEN-CNEN/SP by employing modern national components and expertise. (author)

  2. A new electronic circuit for NIM rack power supply

    Energy Technology Data Exchange (ETDEWEB)

    Toledo, Fabio de; Domienikan, Claudio; Zahn, Guilherme S., E-mail: fatoledo@ipen.br, E-mail: clanikan@ipen.br, E-mail: gzahn@ipen.br [Intituto de Pesquisas Energeticas e Nucleares (IPEN-CNEN/SP), Sao Paulo, SP (Brazil)

    2013-07-01

    A new and useful electronic circuit designed to substitute the old power supply of IPEN model PM 1212 Nuclear Instrument Modules (NIM) racks is presented. Originally, these NIM racks were projected by IPEN workshop at 1974 to be used in nuclear research laboratories. The PM1212 provided mounting space and power sources for up to 12 standard nuclear electronic modules and power supply regulated DC voltages of ±24V (2A), ±12V (4A) and unregulated AC 117V (0.5A). Currently, several of these NIM racks are not in use because the electronic components of the original design have become obsolete and are no longer manufactured. The new electronic circuit provides the same original voltages adjusted in the single circuit and, additionally, ±6V. The new power supply was designed and constructed at IPEN-CNEN/SP by employing modern national components and expertise. (author)

  3. Engineering analyses of ITER divertor diagnostic rack design

    Energy Technology Data Exchange (ETDEWEB)

    Modestov, Victor S., E-mail: modestov@compmechlab.com [St Petersburg State Polytechnical University, 195251 St Petersburg, 29 Polytechnicheskaya (Russian Federation); Nemov, Alexander S.; Borovkov, Aleksey I.; Buslakov, Igor V.; Lukin, Aleksey V. [St Petersburg State Polytechnical University, 195251 St Petersburg, 29 Polytechnicheskaya (Russian Federation); Kochergin, Mikhail M.; Mukhin, Eugene E.; Litvinov, Andrey E.; Koval, Alexandr N. [Ioffe Physico-Technical Institute, 194021 St Petersburg, 26 Polytechnicheskaya (Russian Federation); Andrew, Philip [ITER Organization, Route de Vinon sur Verdon, 13115 Saint Paul Lez Durance (France)

    2013-10-15

    Highlights: • The approach developed early has been used for the assessment of new design of DTS racks and neutron shield units. • Results of most critical EM and seismic analyses indicate that introduced changes significantly improved the system behaviour under these loads. • However further research is required to finalize the design and check it upon meeting all structural, thermal, seismic, EM and fatigue requirements. -- Abstract: The divertor port racks used as a support structure of the divertor Thomson scattering equipment has been carefully analyzed to be consistent with electromagnetic and seismic loads. It follows from the foregoing simulations that namely these analyses demonstrate critical challenges associated with the structure design. Based on the results of the reference structure [2] a modified design of the diagnostic racks is proposed and updated simulation results are given. The results signify a significant improvement over the previous reference layout and the design will be continued towards finalization.

  4. CM-2 Environmental/Modal Testing of SPACEHAB Racks

    Science.gov (United States)

    McNelis, Mark E.; Goodnight, Thomas W.

    2001-01-01

    Combined environmental/modal vibration testing has been implemented at the NASA Glenn Research Center's Structural Dynamics Laboratory. The benefits of combined vibration testing are that it facilitates test article modal characterization and vibration qualification testing. The Combustion Module-2 (CM-2) is a space experiment that will launch on shuttle mission STS-107 in the SPACEHAB Research Double Module. The CM-2 flight hardware is integrated into a SPACEHAB single and double rack. CM-2 rack-level combined vibration testing was recently completed on a shaker table to characterize the structure's modal response and verify the random vibration response. Control accelerometers and limit force gauges, located between the fixture and rack interface, were used to verify the input excitation. Results of the testing were used to verify the loads and environments for flight on the shuttles.

  5. [Sciatica. From stretch rack to microdiscectomy].

    Science.gov (United States)

    Gruber, P; Böni, T

    2015-12-01

    In ancient times as well as in the Middle Ages treatment options for discogenic nerve compression syndrome were limited and usually not very specific because of low anatomical and pathophysiological knowledge. The stretch rack (scamnum Hippocratis) was particularly prominent but was widely used as a therapeutic device for very different spinal disorders. Since the beginning of the nineteenth century anatomical knowledge increased and the advances in the fields of asepsis, anesthesia and surgery resulted in an increase in surgical interventions on the spine. In 1908 the first successful lumbar discectomy was initiated and performed by the German neurologist Heinrich O. Oppenheim (1858-1919) and the surgeon Fedor Krause (1857-1937); however, neither recognized the true pathological condition of discogenic nerve compression syndrome. With the landmark report in the New England Journal of Medicine in 1934, the two American surgeons William Jason Mixter (1880-1958) and Joseph Seaton Barr (1901-1963) finally clarified the pathomechanism of lumbar disc herniation and furthermore, propagated discectomy as the standard therapy. Since then interventions on intervertebral discs rapidly increased and the treatment options for lumbar disc surgery quickly evolved. The surgical procedures changed over time and were continuously being refined. In the late 1960s the surgical microscope was introduced for spinal surgery by the work of the famous neurosurgeon Mahmut Gazi Yasargil and his colleague Wolfhard Caspar and so-called microdiscectomy was introduced. Besides open discectomy other interventional techniques were developed to overcome the side effects of surgical procedures. In 1964 the American orthopedic surgeon Lyman Smith (1912-1991) introduced chemonucleolysis, a minimally invasive technique consisting only of a cannula and the proteolytic enzyme chymopapain, which is injected into the disc compartment to dissolve the displaced disc material. In 1975 the Japanese orthopedic

  6. Two-sensor control in active vibration isolation using hard mounts

    NARCIS (Netherlands)

    Beijen, M.A.; Tjepkema, D.; van Dijk, J.

    To isolate precision machines from floor vibrations, active vibration isolators are often applied. In this paper, a two-sensor control strategy, based on acceleration feedback and force feedback, is proposed for an active vibration isolator using a single-axis active hard mount. The hard mount

  7. Two-sensor control in active vibration isolation using hard mounts

    NARCIS (Netherlands)

    Beijen, M.A.; Tjepkema, D.; van Dijk, Johannes

    2014-01-01

    To isolate precision machines from floor vibrations, active vibration isolators are often applied. In this paper, a two-sensor control strategy, based on acceleration feedback and force feedback, is proposed for an active vibration isolator using a single-axis active hard mount. The hard mount

  8. Antiproliferative Activity of Phenylpropanoids Isolated from Lagotis brevituba Maxim.

    Science.gov (United States)

    Xiang, Yuan; Jing, Zhao; Haixia, Wang; Ruitao, Yu; Huaixiu, Wen; Zenggen, Liu; Lijuan, Mei; Yiping, Wang; Yanduo, Tao

    2017-10-01

    The aim of the present study was to evaluate the antiproliferative effect of phenylpropanoids isolated from the n-BuOH-soluble fraction of an ethanolic extract of Lagotis brevituba Maxim. The phenylpropanoids were identified as echinacoside, lagotioside, glucopyranosyl(1-6)martynoside, plantamoside, and verbascoside. Three of the compounds, lagotioside, glucopyranosyl(1-6)martynoside, and plantamoside, were isolated from L. brevituba for the first time. The antiproliferative activity of the isolates was evaluated in human gastric carcinoma (MGC-803), human colorectal carcinoma (HCT116), human hepatocellar carcinoma (HepG2), and human lung cancer (HCT116) cells using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Plantamoside showed promising activity against MGC-803 cells, with a half maximal inhibitory concentration value of 37.09 μM. The mechanism of the pro-apoptosis effect of plantamoside was then evaluated in MGC-803 cells. Changes in cell morphology, including disorganization of the architecture of actin microfilaments and formation of apoptotic bodies, together with cell cycle arrest in G2/M phases, were observed after treatment of plantamoside. The antiproliferative and pro-apoptotic effects were associated with a decrease in the ratio of Bcl-2/Bax and reduced mitochondrial membrane potential, which was accompanied by the release of reactive oxygen species and Ca 2+ into the cytoplasm. Taken together, the results indicated that plantamoside promotes apoptosis via a mitochondria-dependent mechanism. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  9. Antagonistic Activity Of Endophytic Bacteria Isolated From Mentha Rotundifolia L.

    Directory of Open Access Journals (Sweden)

    Elhartiti Abla

    2015-08-01

    Full Text Available Abstract This study is implemented for the isolation purification and identification of endophytic bacteria which produces antifungal substances from the roots of Mentha rotundifolia L. The 59 obtained bacterial isolates were tested for their antagonistic activity by the dual confrontation against the phytopathogenic fungi Fusarium oxysporum Aspergillus Niger and Botrytis cinerea. Eight bacterial strains were selected for their strong antifungal activity. These are strains M21 M23 M3a M4 M14d and M3c which belong to the family Bacillaceae M12 and M3b which belongs to the family of Pseudomonadaceae. Among these three bacterial strains namely M21 M23 and M12 induce 70 of inhibition of mycelial growth of phytopathogenic fungi Fusarium oxysporum and Aspergillus Niger while the five bacterial strains M3a M3c M3b M4 and M14d have proved to be effective in inhibiting more than 60 of mycelial growth of Botrytis cinerea.

  10. GCM2-Activating Mutations in Familial Isolated Hyperparathyroidism.

    Science.gov (United States)

    Guan, Bin; Welch, James M; Sapp, Julie C; Ling, Hua; Li, Yulong; Johnston, Jennifer J; Kebebew, Electron; Biesecker, Leslie G; Simonds, William F; Marx, Stephen J; Agarwal, Sunita K

    2016-11-03

    Primary hyperparathyroidism (PHPT) is a common endocrine disease characterized by parathyroid hormone excess and hypercalcemia and caused by hypersecreting parathyroid glands. Familial PHPT occurs in an isolated nonsyndromal form, termed familial isolated hyperparathyroidism (FIHP), or as part of a syndrome, such as multiple endocrine neoplasia type 1 or hyperparathyroidism-jaw tumor syndrome. The specific genetic or other cause(s) of FIHP are unknown. We performed exome sequencing on germline DNA of eight index-case individuals from eight unrelated kindreds with FIHP. Selected rare variants were assessed for co-segregation in affected family members and screened for in an additional 32 kindreds with FIHP. In eight kindreds with FIHP, we identified three rare missense variants in GCM2, a gene encoding a transcription factor required for parathyroid development. Functional characterization of the GCM2 variants and deletion analyses revealed a small C-terminal conserved inhibitory domain (CCID) in GCM2. Two of the three rare variants were recurrent, located in the GCM2 CCID, and found in seven of the 40 (18%) kindreds with FIHP. These two rare variants acted as gain-of-function mutations that increased the transcriptional activity of GCM2, suggesting that GCM2 is a parathyroid proto-oncogene. Our results demonstrate that germline-activating mutations affecting the CCID of GCM2 can cause FIHP. Published by Elsevier Inc.

  11. Biofilm-forming activity of bacteria isolated from toilet bowl biofilms and the bactericidal activity of disinfectants against the isolates.

    Science.gov (United States)

    Mori, Miho; Gomi, Mitsuhiro; Matsumune, Norihiko; Niizeki, Kazuma; Sakagami, Yoshikazu

    2013-01-01

    To evaluate the sanitary conditions of toilets, the bacterial counts of the toilet bowl biofilms in 5 Kansai area and 11 Kansai and Kanto area homes in Japan were measured in winter and summer seasons, respectively. Isolates (128 strains) were identified by analyzing 16S ribosomal RNA sequences. The number of colonies and bacterial species from biofilms sampled in winter tended to be higher and lower, respectively, than those in summer. Moreover, the composition of bacterial communities in summer and winter samples differed considerably. In summer samples, biofilms in Kansai and Kanto areas were dominated by Blastomonas sp. and Mycobacterium sp., respectively. Methylobacterium sp. was detected in all toilet bowl biofilms except for one sample. Methylobacterium sp. constituted the major presence in biofilms along with Brevundimonas sp., Sphingomonas sp., and/or Pseudomonas sp. The composition ratio of the sum of their genera was 88.0 from 42.9% of the total bacterial flora. The biofilm formation abilities of 128 isolates were investigated, and results suggested that Methylobacterium sp. and Sphingomonas sp. were involved in biofilm formation in toilet bowls. The biofilm formation of a mixed bacteria system that included bacteria with the highest biofilm-forming ability in a winter sample was greater than mixture without such bacteria. This result suggests that isolates possessing a high biofilm-forming activity are involved in the biofilm formation in the actual toilet bowl. A bactericidal test against 25 strains indicated that the bactericidal activities of didecyldimethylammonium chloride (DDAC) tended to be higher than those of polyhexamethylene biguanide (PHMB) and N-benzyl-N,N-dimethyldodecylammonium chloride (ADBAC). In particular, DDAC showed high bactericidal activity against approximately 90% of tested strains under the 5 h treatment.

  12. Manzamine alkaloids: isolation, cytotoxicity, antimalarial activity and SAR studies.

    Science.gov (United States)

    Ashok, Penta; Ganguly, Swastika; Murugesan, Sankaranarayanan

    2014-11-01

    The infectious disease Malaria is caused by different species of the genus Plasmodium. Resistance to quinoline antimalarial drugs and decreased susceptibility to artemisinin-based combination therapy have increased the need for novel antimalarial agents. Historically, natural products have been used for the treatment of infectious diseases. Identification of natural products and their semi-synthetic derivatives with potent antimalarial activity is an important method for developing novel antimalarial agents. Manzamine alkaloids are a unique group of β-carboline alkaloids isolated from various species of marine sponge displaying potent antimalarial activity against drug-sensitive and -resistant strains of Plasmodium. In this review, we demonstrate antimalarial potency, cytotoxicity and antimalarial SAR of manzamine alkaloids. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. ISS Payload Racks Automated Flow Control Calibration Method

    Science.gov (United States)

    Simmonds, Boris G.

    2003-01-01

    Payload Racks utilize MTL and/or LTL station water for cooling of payloads and avionics. Flow control range from valves of fully closed, to up to 300 Ibmhr. Instrument accuracies are as high as f 7.5 Ibm/hr for flow sensors and f 3 Ibm/hr for valve controller, for a total system accuracy of f 10.5 Ibm/hr. Improved methodology was developed, tested and proven that reduces accuracy of the commanded flows to less than f 1 Ibmhr. Uethodology could be packed in a "calibration kit" for on- orbit flow sensor checkout and recalibration, extending the rack operations before return to earth. -

  14. Solar and interplanetary activities of isolated and non-isolated coronal mass ejections

    Science.gov (United States)

    Bendict Lawrance, M.; Shanmugaraju, A.; Moon, Y.-J.; Umapathy, S.

    2017-07-01

    We report our results on comparison of two halo Coronal Mass Ejections (CME) associated with X-class flares of similar strength (X1.4) but quite different in CME speed and acceleration, similar geo-effectiveness but quite different in Solar Energetic Particle (SEP) intensity. CME1 (non-isolated) was associated with a double event in X-ray flare and it was preceded by another fast halo CME of speed = 2684 km/s (pre-CME) associated with X-ray flare class X5.4 by 1 h from the same location. Since this pre-CME was more eastern, interaction with CME1 and hitting the earth were not possible. This event (CME1) has not suffered the cannibalism since pre-CME has faster speed than post-CME. Pre-CME plays a very important role in increasing the intensity of SEP and Forbush Decrease (FD) by providing energetic seed particles. So, the seed population is the major difference between these two selected events. CME2 (isolated) was a single event. We would like to address on the kinds of physical conditions related to such CMEs and their associated activities. Their associated activities such as, type II bursts, SEP, geomagnetic storm and FD are compared. The following results are obtained from the analysis. (1) The CME leading edge height at the start of metric/DH type II bursts are 2 R⊙/ 4 R⊙ for CME1, but 2 R⊙/ 2.75 R⊙ for CME2. (2) Peak intensity of SEP event associated with the two CMEs are quite different: 6530 pfu for CME1, but 96 pfu for CME2. (3) The Forbush decrease occurred with a minimum decrease of 9.98% in magnitude for CME1, but 6.90% for CME2. (4) These two events produced similar intense geomagnetic storms of intensity of Dst index -130 nT. (5) The maximum southward magnetic fields corresponding to Interplanetary CME (ICME) of these two events are nearly the same, but there is difference in Sheath Bz maximum (-14.2, -6.9 nT). (6) The time-line chart of the associated activities of two CMEs show some difference in the time delay between the onsets of

  15. Evaluating Antimutagenic Activity of Probiotic Bacteria Isolated from Probiotic Products

    Directory of Open Access Journals (Sweden)

    R Kazemi Darsanki

    2012-07-01

    Full Text Available

    Background and Objectives: Probiotic bacteria are microbial nutrition supplements which have useful effects on human health by maintaining of bowel microbial balance. There are many studies that have been suggested the use of probiotic products as cancer risk reducer. The aim of this study, is isolation and detection of probiotic agents from yoghurt and probiotical tablet and evaluation of their abilities to decrease some effects of mutagenic and carcinogenic agents.

     

    Methods: In this study, probiotic bacteria were isolated from yogurt and probiotic tablet by using MRS in anaerobic condition (5% Co2 and gas peck and temperature of 37°c. Then, they were detected by using biochemical tests. Their anti mutagenic effects of supernatant culture were evaluated against mutagenic agents of azid Sodium and Potassium Permanganate by ames test (Salmonella typhimurium TA100 in presence and absence of S9.

     

    Results: Six probiotic bacteria were isolated from yogurt and probiotic tablet. Their anti mutagenic activity results based on ames test showed they can inhibit mutagenic agents more than 40% in some species, which is considered as a good result.

     

    Conclusion: The results of this study show that the use of probiotic bacteria found in different products such as yogurt and probiotic tablets, have proper anti mutagenic and anti carcinogenic effects. They change the micro flora of bowel and, as a result, reduce absorption of mutagenic and carcinogenic agents and help to maintain human health.

     

  16. 26 CFR 48.4081-2 - Taxable fuel; tax on removal at a terminal rack.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 16 2010-04-01 2010-04-01 true Taxable fuel; tax on removal at a terminal rack..., Tread Rubber, and Taxable Fuel Taxable Fuel § 48.4081-2 Taxable fuel; tax on removal at a terminal rack... rack are subject to tax and the position holder with respect to the fuel is liable for the tax. (b...

  17. 78 FR 54450 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Preliminary...

    Science.gov (United States)

    2013-09-04

    ... Shelving and Racks From the People's Republic of China: Preliminary Results of Antidumping Duty... the antidumping duty order on certain kitchen appliance shelving and racks from the People's Republic...\\ is certain kitchen appliance shelving and racks. The subject merchandise is currently classifiable...

  18. 77 FR 61385 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Antidumping...

    Science.gov (United States)

    2012-10-09

    ... Shelving and Racks From the People's Republic of China: Antidumping Duty Administrative Review, 2010-2011... administrative review of the antidumping duty order on certain kitchen appliance shelving and racks from the... INFORMATION: Scope of the Order The scope of this order consists of shelving and racks for refrigerators...

  19. 77 FR 72324 - Certain Kitchen Appliance Shelving and Racks from the People's Republic of China: Countervailing...

    Science.gov (United States)

    2012-12-05

    ... Shelving and Racks from the People's Republic of China: Countervailing Duty Administrative Review, 2010... countervailing duty order on certain kitchen appliance shelving and racks from the People's Republic of China. See Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Countervailing...

  20. 77 FR 12811 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Partial...

    Science.gov (United States)

    2012-03-02

    ... Shelving and Racks From the People's Republic of China: Partial Rescission of Antidumping Duty... review of the antidumping duty order on certain kitchen appliance shelving and racks from the People's... Appliance Shelving and Racks from the People's Republic of China, dated January 10, 2012; and Letter to the...

  1. White House Communications Agency (WHCA) Presidential Voice Communications Rack Mount System Mechanical Drawing Package

    Science.gov (United States)

    2015-12-01

    Rack Mount System Mechanical Drawing Package by Steven P Callaway Approved for public release; distribution unlimited...Laboratory White House Communications Agency (WHCA) Presidential Voice Communications Rack Mount System Mechanical Drawing Package by Steven P...Note 3. DATES COVERED (From - To) 04/2013 4. TITLE AND SUBTITLE White House Communications Agency (WHCA) Presidential Voice Communications Rack

  2. 40 CFR 63.2475 - What requirements must I meet for transfer racks?

    Science.gov (United States)

    2010-07-01

    ... transfer racks? 63.2475 Section 63.2475 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... must I meet for transfer racks? (a) You must comply with each emission limit and work practice standard in table 5 to this subpart that applies to your transfer racks, and you must meet each applicable...

  3. 76 FR 62765 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Preliminary...

    Science.gov (United States)

    2011-10-11

    ... Shelving and Racks From the People's Republic of China: Preliminary Results of the First Administrative... review of the antidumping duty order on certain kitchen appliance shelving and racks from the People's... administrative review of certain kitchen appliance shelving and racks from the PRC for the period March 5, 2009...

  4. Antifouling Activity of Secondary Metabolites Isolated from Chinese Marine Organisms

    KAUST Repository

    Li, Yong Xin

    2013-04-25

    Biofouling results in tremendous economic losses to maritime industries around the world. A recent global ban on the use of organotin compounds as antifouling agents has further raised demand for safe and effective antifouling compounds. In this study, 49 secondary metabolites, including diterpenoids, steroids, and polyketides, were isolated from soft corals, gorgonians, brown algae, and fungi collected along the coast of China, and their antifouling activity was tested against cyprids of the barnacle Balanus (Amphibalanus) amphitrite. Twenty of the compounds were found to inhibit larval settlement significantly at a concentration of 25 μg ml-1. Two briarane diterpenoids, juncin O (2) and juncenolide H (3), were the most promising non-toxic antilarval settlement candidates, with EC50 values less than 0.13 μg ml-1 and a safety ratio (LC50/EC50) higher than 400. A preliminary structure-activity relationships study indicated that both furanon and furan moieties are important for antifouling activity. Intriguingly, the presence of hydroxyls enhanced their antisettlement activity. © 2013 Springer Science+Business Media New York.

  5. Antifouling Activity of Secondary Metabolites Isolated from Chinese Marine Organisms

    KAUST Repository

    Li, Yong Xin; Wu, Hui Xian; Xu, Ying; Shao, Chang Lun; Wang, Chang Yun; Qian, Pei Yuan

    2013-01-01

    Biofouling results in tremendous economic losses to maritime industries around the world. A recent global ban on the use of organotin compounds as antifouling agents has further raised demand for safe and effective antifouling compounds. In this study, 49 secondary metabolites, including diterpenoids, steroids, and polyketides, were isolated from soft corals, gorgonians, brown algae, and fungi collected along the coast of China, and their antifouling activity was tested against cyprids of the barnacle Balanus (Amphibalanus) amphitrite. Twenty of the compounds were found to inhibit larval settlement significantly at a concentration of 25 μg ml-1. Two briarane diterpenoids, juncin O (2) and juncenolide H (3), were the most promising non-toxic antilarval settlement candidates, with EC50 values less than 0.13 μg ml-1 and a safety ratio (LC50/EC50) higher than 400. A preliminary structure-activity relationships study indicated that both furanon and furan moieties are important for antifouling activity. Intriguingly, the presence of hydroxyls enhanced their antisettlement activity. © 2013 Springer Science+Business Media New York.

  6. Development of a cosmic rack for characterization of RPCs

    International Nuclear Information System (INIS)

    Sehgal, S.T.; Pant, L.M.

    2011-01-01

    A newly designed cosmic rack has been commissioned this year in the RPC Lab., in NPD-BARC. The rack consists of nine shelves each providing a clear area of 160 cm x 225 cm. Each of the shelf is separated by a distance of 30 cm, providing sufficient distance for fast electronics to respond and also providing an easy access for loading and removing the RPCs. The total height of the cosmic rack is 2.8 metres. A typical fully integrated RPCs covers an approximate area of 2 m 2 and weighs about 60-70 kg. The rack has been basically designed, keeping in mind the varied requirements as regards to the RPCs based current and futuristic programmes which are listed as follows: 1. Testing of large area trapezoidal bakelite gas-gaps and RPCs for the CMS experiment, keeping in mind the production rate of five RPCs per month. 2. The proposed muon tomography programme with 1m x 1m glass RPCs and 3. Characterization of glass RPCs (1m x 2m) for the INO experiment in near future

  7. Rollin' in Style!: Students Design Bike Mounted Skateboard Racks

    Science.gov (United States)

    Massey, Rick

    2008-01-01

    Recognizing the increasing popularity of skateboarding, the author has found a project that teaches design and manufacturing concepts--and, of equal importance, really gets his students motivated. He challenges them to design and build a skateboard rack that mounts easily on a bicycle. The project benefits students by teaching creativity, the…

  8. Three-dimensional seismic analysis for spent fuel storage rack

    International Nuclear Information System (INIS)

    Lee, Gyu Mahn; Kim, Kang Soo; Park, Keun Bae; Park, Jong Kyun

    1998-01-01

    Time history analysis is usually performed to characterize the nonlinear seismic behavior of a spent fuel storage rack (SFSR). In the past, the seismic analyses of the SFSR were performed with two-dimensional planar models, which could not account for torsional response and simultaneous multi-directional seismic input. In this study, three-dimensional seismic analysis methodology is developed for the single SFSR using the ANSY code. The 3-D model can be used to determine the nonlinear behavior of the rack, i.e., sliding, uplifting, and impact evaluation between the fuel assembly and rack, and rack and the pool wall. This paper also reviews the 3-D modeling of the SFSR and the adequacy of the ANSYS for the seismic analysis. As a result of the adequacy study, the method of ANSYS transient analysis with acceleration time history is suitable for the seismic analysis of highly nonlinear structure such as an SFSR but it isn't appropriate to use displacement time history of seismic input. (author)

  9. Replacement of the rotary specimen rack in the U.S. Geological Survey TRIGA Reactor (GSTR)

    International Nuclear Information System (INIS)

    Rusling, D.H.; Helfer, P.G.; Perryman, R.E.; Smith, W.L.

    1986-01-01

    A new rotary specimen rack was installed in the GSTR on November 13, 1985. The new rack has been modified to allow the pneumatic unloading of samples. Instructions for the removal and installation of the rotary specimen rack were provided by GA Technologies. The first step was to prepare the core for removal of the control rods, T/C fuel elements, and the central thimble. The rack was weighted prior to removal to ensure that it could not float to the surface. The old rack was stored at the side of the tank. Radiation measurements were made with an underwater ion chamber. (author)

  10. Antioxidant activity of probiotic lactic acid bacteria isolated from Mongolian airag

    OpenAIRE

    E Uugantsetseg; B Batjargal

    2014-01-01

    This research aimed to determine the antioxidant activity of probiotic lactic acid bacteria isolated from airag. In this study, 42 lactic acid bacteria were isolated from Mongolian airag. All isolates were identified by using morphological, biochemical and physiological methods. The isolated bacteria were studied for antagonistic effects on Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, 22 strains showed antibacterial activity. When we examined thei...

  11. Safety Assessment of Multi Purpose Small Payload Rack(MSPR)

    Science.gov (United States)

    Mizutani, Yoshinobu; Takada, Satomi; Murata, Kosei; Ozawa, Daisaku; Kobayashi, Ryoji; Nakamura, Yasuhiro

    2010-09-01

    We are reporting summary of preliminary safety assessment for Multi Purpose Small Payload Rack(MSPR), which is one of the micro gravity experiment facilities that are being developed for the 2nd phase JEM utilization(JEM: Japanese Experiment Module) that will be launched on H-II Transfer Vehicle(HTV) 2nd flight in 2011. MSPR is used for multi-purpose micro-g experiment providing experimental spaces and work stations. MSPR has three experimental spaces; first, there is a space called Work Volume(WV) with capacity volume of approximately 350 litters, in which multiple resources including electricity, communication, and moving image functions can be used. Within this space, installation of devices can be done by simple, prompt attachment by Velcro and pins with high degree of flexibility. Second, there is Small Experiment Area(SEA), with capacity volume of approximately 70 litters, in which electricity, communication, and moving image functions can also be used in the same way as WV. These spaces protect experiment devices and specimens from contingent loads by the crewmembers. Third, there is Work Bench with area of 0.5 square meters, on which can be used for maintenance, inspection and data operations of installed devices, etc. This bench can be stored in the rack during contingency. Chamber for Combustion Experiment(CCE) that is planned to be installed in WV is a pressure-resistant experimental container that can be used to seal hazardous materials from combustion experiments. This CCE has double sealing design in chamber itself, which resist gas leakage under normal the temperature and pressure. Electricity, communication, moving image function can be used in the same way as WV. JAXA Phase 2 Safety Review Panel(SRP) has been held in April, 2010. For safety analysis of MSPR, hazards were identified based on Fault Tree Analysis methodology and then these hazards were classified into either eight ISS standard-type hazards or eight unique-type hazards that requires

  12. 77 FR 21744 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Final Results...

    Science.gov (United States)

    2012-04-11

    ... Shelving and Racks From the People's Republic of China: Final Results of the Countervailing Duty... countervailable duty order on certain kitchen appliance shelving and racks (``Kitchen Racks'') from the People's... Kitchen Appliance Shelving and Racks from the People's Republic of China: Preliminary Results of...

  13. 40 CFR Table 2 to Subpart Bbbbbb... - Applicability Criteria, Emission Limits, and Management Practices for Loading Racks

    Science.gov (United States)

    2010-07-01

    ... Limits, and Management Practices for Loading Racks 2 Table 2 to Subpart BBBBBB of Part 63 Protection of... Criteria, Emission Limits, and Management Practices for Loading Racks If you own or operate Then you must 1. A gasoline loading rack(s) at a bulk gasoline terminal with a gasoline throughput of 250,000 gallons...

  14. Antibacterial activity of garlic and lime on isolates of extracted ...

    African Journals Online (AJOL)

    ) on seven bacterial species (Streptococcus mutans, Lactobacillus acidophilus, Norcadia asteroides, Pseudomonas aeruginosa, Actinomyces viscosus, Staphylococcus aureus and Veillonella alcaligens) isolated from 240 extracted, carious ...

  15. Thermal neutron flux measurements in the rotary specimen rack of the IPR-R1 TRIGA reactor

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Rose Mary G. do Prado; Rodrigues, Rogério R.; Souza, Luiz Claudio A., E-mail: souzarm@cdtn.br, E-mail: rrr@cdtn.br, E-mail: lcas@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2017-07-01

    The thermal neutron flux in the rotary specimen rack of the IPR-R1 TRIGA reactor at the Nuclear Technology Development Center (CDTN), Belo Horizonte, Brazil, has been measured by the neutron activation method, using bare and cadmium covered gold foils. Those foils were irradiated in the rotary specimen rack with the reactor at 100 kW. The reactor core configuration has 63 fuel elements, composed of 59 original aluminum-clad elements and 4 stainless steel-clad fuel elements. The gamma activities of the foils were measured using Ge spectrometer. The perturbations of the thermal neutron flux caused by the introduction of an absorbing foil into the medium were considered in order to obtain accurate determination of the flux. The thermal neutron flux obtained was 7.4 x 10{sup 11} n.cm{sup -2}.s{sup -1}. (author)

  16. Melting of fuel element racks and their recycling as granulate

    International Nuclear Information System (INIS)

    Quade, U.; Kluth, T.; Kreh, R.

    1998-01-01

    In order to increase the storage capacity for spent fuel elements in the Spanish NPPs of Almaraz and Asco, the existing racks were replaced by compact one in 1991/1993. The 28 racks from Almaraz NPP were cut on site, packed in 200-I-drums and taken to intermediate storage. For the remaining 28 racks of Asco NPP, ENRESA preferred the melting alternative. To demonstrate the recycling path melting in Germany, a test campaign with six racks was performed in 1997. As a result of this test melt, the limits for Carla melting plant were modified to 200 Bq/g total, α, β, γ 100 Bq/g nuclear fuels, max. 3g/100 kg 2,000 Bq/g total Fe55, H 3 , C-14 and Ni63. After the test melt campaign, the German authorities licensed the import and treatment of the remaining 22 racks on the condition that the waste resulting from the melting process as well as the granules produced were taken back to Spain. The shipment from Asco via France to Germany has been carried out in F 20-ft-IPII containers in accordance with ADR. Size reduction to chargeable dimensions was carried out by a plasma burner and hydraulic shears. For melting, a 3.2 Mg medium frequency induction furnace, operated in a separate housing, was used. For granules production outside this housing, the liquid iron was cast into a 5Mg ladle and then, through a water jet, into the granulating basin. The total mass of 287,659 Kg of 28 fuel elements racks and components of the storage basin yielded 297,914 kg of iron granulate. Secondary waste from melting amounted to 9,920 kg, corresponding to 3.45% of the input mass. The granulating process produced 6,589 kg, corresponding to 2.28% of the total mass to be melted. Radiological analysis of samples taken from the melt and different waste components confirmed the main nuclides Co60, Cs134 and Cs137. Fe55 was highly overestimated by the preliminary analysis. (Author) 2 refs

  17. Head losses in small hydropower plant trash racks (SHP

    Directory of Open Access Journals (Sweden)

    N. Walczak

    2016-12-01

    Full Text Available Small hydropower plants (SHP are technical facilities that are part of alternative energy sources [Paish 2002]. They are primarily characterised by low unit power (in Poland below 5 MW and are often constructed on existing barrages. Electrical current produced by these plants is used to meet local demand. Considering the exploitation of SHPs, it is important to ensure a stable flow through turbines. Aggidis et al. [2010] analysed SHP equipment costs depending on the turbine set. The turbines are protected against damage with trash racks applied for capturing water-borne detritus, such as plant debris carried by water. However, trash racks as solid equipment of SHPs cause head losses, and as a consequence reduce the efficiency of the system. These losses result not only from the spacing of bars, their shape and the technical condition of the inlet chamber, but also from plant debris, its nature, and the quantity of accumulated material that effectively limits the flow. The plant debris captured on trash racks is characterised by diversity in terms of species composition related to the vegetation period and the area where hydraulic facilities are located. Therefore, it is important to maintain trash racks clean by regular removal of the accumulated material. In this context, modernised and newly built power plants are fitted with mechanical cleaners. In older facilities, manual intervention for regular cleaning is required. The present study analyses how the bar shape and the orientation angle of trash racks as well as the accumulated plant debris affect head losses. The results were obtained from laboratory tests. The research examined the impact the inclination angle of trash racks (30°, 60° and 80° has on head loss values for three different shapes of bars (cylindrical, angled and flat rectangular and various weight portions of plant debris (0.25, 0.375 and 0.5 kg. The summarised losses were determined by measuring the difference in water

  18. Antischistosomal Activity of Two Active Constituents Isolated from the Leaves of Egyptian Medicinal Plants

    Directory of Open Access Journals (Sweden)

    Sanaa A. Ali

    2015-01-01

    Full Text Available Highlights D -mannitol, a naturally occurring sugar isolated from the leaves Ixora undulata Roxb., and a linear chain pectin homogalacturonan (HG polysaccharide isolated from the leaves of Linum grandiflorum Desf. (scarlet flax were evaluated for their therapeutic effect against schistosomiasis with biochemical and histochemical evaluations, and compared with the reference drug praziquantel, to assess the antioxidant and antischistosomal effects of D -mannitol and pectin. Abstract In this paper, we investigate the role of two active constituents isolated from the leaves of Egyptian medicinal plants. D -mannitol a naturally occurring sugar isolated from the leaves Ixora undulata Roxb., and the pectin a linear chain homogalacturonan (HG polysaccharide isolated from the leaves of Linum grandiflorum Desf. (scarlet flax. Both are evaluated for their therapeutic effect against schistosomiasis with biochemical and histochemical evaluations and compared with praziquantel, a reference drug. Biochemical studies of hepatic glucose, the glycogen content, and total serum protein were carried out, and histochemical evaluations through serum protein fractions separated by polyacrylamide gel electrophoresis with different molecular weights (260–10 kDa were made in all groups, in addition to liver and body weight. D -mannitol and pectin show a remarkable effect in enhancing liver and kidney functions through enhancing most protein fractions in the serum of mice infected with Schistosoma mansoni. Also, the glucose and glycogen content in injured liver tissues improved, in addition liver and body weight in the infected groups. Thus they may be of therapeutic potential in the treatment hepatoxicity and nephrotoxicity.

  19. In vitro anticancer activity of microbial isolates from diverse habitats

    Directory of Open Access Journals (Sweden)

    Angel Treasa Thomas

    2011-06-01

    Full Text Available Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the cytotoxic potential. Nuclear staining and flow cytometric studies were carried out to assess the potential of the extracts in arresting the cell cycle. The crude ethylacetate extract of isolate F-21 showed promising results by MTT assay with IC50 as low as 20.37±0.36 µg/mL on HeLa, and 44.75±0.81 µg/mL on MCF-7 cells, comparable with Cisplatin. The isolate F-21 was identified as Aspergillus sp. Promising results were also obtained with B-2C and B-4E strains. Morphological studies, biochemical tests and preliminary chemical investigation of the extracts were also carried out.Extratos de produtos naturais, especialmente de microrganismos, constituíram-se em fonte valiosa de diversas moléculas em muitas descobertas de fármacos e levaram à descoberta de fármacos importantes. A identificação de espécies microbianas que apresentam atividade biológica e a purificação de biomoléculas responsáveis pelas atividades levou à descoberta de muitas moléculas bioativas. Extratos extracelulares tanto quanto intracelulares de metabólitos de 36 isolados de bactérias e 24 isolados de fungos, que cresceram sob condições não usuais, como alta temperatura, alta concentração de sal e baixa

  20. Antioxidant and Antiplasmodial Activities of Bergenin and 11-O-Galloylbergenin Isolated from Mallotus philippensis

    OpenAIRE

    Khan, Hamayun; Amin, Hazrat; Ullah, Asad; Saba, Sumbal; Rafique, Jamal; Khan, Khalid; Ahmad, Nasir; Badshah, Syed Lal

    2016-01-01

    Two important biologically active compounds were isolated from Mallotus philippensis. The isolated compounds were characterized using spectroanalytical techniques and found to be bergenin (1) and 11-O-galloylbergenin (2). The in vitro antioxidant and antiplasmodial activities of the isolated compounds were determined. For the antioxidant potential, three standard analytical protocols, namely, DPPH radical scavenging activity (RSA), reducing power assay (RPA), and total antioxidant capacity (T...

  1. Near-Source Error Sensor Strategies for Active Vibration Isolation of Machines

    NARCIS (Netherlands)

    Beijers, C.A.J.; Basten, T.G.H.; de Boer, Andries; van den Brink, D.R.; Verheij, J.W.; Sas, P; de Munck, M.

    2004-01-01

    Due to lightweight construction of vehicles and ships, the reduction of structure borne interior noise problems with passive isolation of engine vibrations might be not sufficient. To improve the isolation, a combination of passive and active isolation techniques can be used (so-called hybrid

  2. Antimicrobial activity screening of isolated flavonoids from Azadirachta indica leaves

    Directory of Open Access Journals (Sweden)

    QUDSIA KANWAL

    2011-03-01

    Full Text Available The antimicrobial activities of two flavonoids, namely genistein 7-O-glucoside (1 and (–-epi-catechin (2, isolated from Azadirachta indica A. Juss (neem leaves, were evaluated against five fungal species, viz: Alternaria alternata (Fr. Keissler, Aspergillus fumigatus Fresenius, Aspergillus niger van Tieghem, Macrophomina phaseolina (Tassi Goid. and Penicillium citrii, and four bacterial species, viz. Lactobacillus sp., Escherichia coli, Azospirillium lipoferum and Bacillus sp. Six concentrations, viz. 100, 300, 500, 700, 900 and 1000 ppm of each of the two flavonoids were employed using malt extract agar medium. All the concentrations of both the test compounds significantly suppressed fungal as well as bacterial growth. The highest concentration (1000 ppm of both fractions 1 and 2 reduced the growth of the different test fungal species by 83–99 % and 82–95 %, respectively. Compound 1 was highly effective against Lactobacillus sp., against which its various concentrations reduced the bacterial growth by 52–99.8 %. Compound 2 was highly effective against A. lipoferum and Bacillus sp., resulting in 94–100 % and 73–99% reduction in bacterial growth, respectively.

  3. Antibacterial and Cytotoxic Activity of Compounds Isolated from Flourensia oolepis

    Directory of Open Access Journals (Sweden)

    Mariana Belén Joray

    2015-01-01

    Full Text Available The antibacterial and cytotoxic effects of metabolites isolated from an antibacterial extract of Flourensia oolepis were evaluated. Bioguided fractionation led to five flavonoids, identified as 2′,4′-dihydroxychalcone (1, isoliquiritigenin (2, pinocembrin (3, 7-hydroxyflavanone (4, and 7,4′-dihydroxy-3′-methoxyflavanone (5. Compound 1 showed the highest antibacterial effect, with minimum inhibitory concentration (MIC values ranging from 31 to 62 and 62 to 250 μg/mL, against Gram-positive and Gram-negative bacteria, respectively. On further assays, the cytotoxic effect of compounds 1–5 was determined by MTT assay on acute lymphoblastic leukemia (ALL and chronic myeloid leukemia (CML cell lines including their multidrug resistant (MDR phenotypes. Compound 1 induced a remarkable cytotoxic activity toward ALL cells (IC50 = 6.6–9.9 μM and a lower effect against CML cells (IC50 = 27.5–30.0 μM. Flow cytometry was used to analyze cell cycle distribution and cell death by PI-labeled cells and by Annexin V/PI staining, respectively. Upon treatment, 1 induced cell cycle arrest in the G2/M phase accompanied by a strong induction of apoptosis. These results describe for the first time the antibacterial metabolites of F. oolepis extract, with 1 being the most effective. This chalcone also emerges as a selective cytotoxic agent against sensitive and resistant leukemic cells, highlighting its potential as a lead compound.

  4. Antimicrobial activity of julifloricine isolated from Prosopis juliflora.

    Science.gov (United States)

    Aqeel, A; Khursheed, A K; Viqaruddin, A; Sabiha, Q

    1989-06-01

    Antimicrobial activity of julifloricine, an alkaloid isolated from Prosopis juliflora, was studied in vitro against 40 microorganisms which included 31 bacteria, two Candida species, five dermatophytic fungi and two viruses. Significant inhibitory effect was noted against Gram positive bacteria. The minimal inhibitory concentration (MIC) for Staphylococcus aureus, S. epidermidis, S. citreus, Streptococcus pyogenes and Sarcina lutea was 1 microgram/ml and against S. faecalis, S. pneumoniae, S. lactis, Corynebacterium diphtheriae, C. hofmannii and Bacillus subtilis, 5 micrograms/ml. Its effect was compared with those of identical concentrations of benzyl penicillin, gentamicin and trimethoprim. The inhibitory effect of julifloricine on Gram negative bacteria such as the species of Salmonella, Shigella, Klebsiella, Proteus, Pseudomonas, Enterobacter, Aeromonas and Vibrio was almost insignificant. Julifloricine as compared to micoanzole was found superior against C. tropicalis and responded equally to C. albicans. As compared to econazole, it was found less effective against both C. albicans and C. tropicalis. This alkaloid was found inactive against dermatophytic fungi (up to a dose of 10 micrograms/ml) and viruses which included Herpes simplex 1 and Newcastle disease virus. Julifloricine up to a doses of 1000 micrograms/25 g of mice was found nonlethal.

  5. Amylase activity of a starch degrading bacteria isolated from soil ...

    African Journals Online (AJOL)

    Starch degrading bacteria are most important for industries such as food, fermentation, textile and paper. Thus isolating and manipulating pure culture from various waste materials has manifold importance for various biotechnology industries. In the present investigation a bacterial strain was isolated from soil sample ...

  6. Rock mechanics activities at the Waste Isolation Pilot Plant

    International Nuclear Information System (INIS)

    Francke, C.; Saeb, S.

    1996-01-01

    The application of rock mechanics at nuclear waste repositories is a true multidisciplinary effort. A description and historical summary of the Waste Isolation Pilot Plant (WIPP) is presented. Rock mechanics programs at the WIPP are outlined, and the current rock mechanics modeling philosophy of the Westinghouse Waste Isolation Division is discussed

  7. activity guided isolation of chemical constituents from the ...

    African Journals Online (AJOL)

    MeOH fraction was subjected to various chromatographic techniques led to the isolation of miquelianin (1), kaempferol 3-O-glucuronide (2) and quercitrin (3). Compounds (1-3) were isolated from this plant for the first time. KEY WORDS: Euphorpia schimperi C. Presl, Wound healing, Chromatographic techniques, Chemical.

  8. Remote inspection of the IFSF spent fuel storage rack

    International Nuclear Information System (INIS)

    Uldrich, E.D.

    1996-01-01

    The Irradiated Fuel Storage Facility (IFSF) is a dry storage facility for spent nuclear fuels located at the Idaho Chemical Processing Plant; it was constructed in the 1970's specifically for the Fort Saint Vrain spent reactor fuels. Currently, it is being used for various spent fuels. It was not known if IFSF would met current DOE seismic criteria, so re-analysis was started, with the rack being analyzed first. The rack was inspected to determine the as-built condition. LazrLyne and VideoRuler were used in lieu of using a tape measure with the camera. It was concluded that when a visual inspection shows widely varying weld sizes, the engineer has to use all resources available to determine the most probable specified weld sizes

  9. Arabidopsis scaffold protein RACK1A modulates rare sugar D-allose regulated gibberellin signaling.

    Science.gov (United States)

    Fennell, Herman; Olawin, Abdulquadri; Mizanur, Rahman M; Izumori, Ken; Chen, Jin-Gui; Ullah, Hemayet

    2012-11-01

    As energy sources and structural components, sugars are the central regulators of plant growth and development. In addition to the abundant natural sugars in plants, more than 50 different kinds of rare sugars exist in nature, several of which show distinct roles in plant growth and development. Recently, one of the rare sugars, D-allose, an epimer of D-glucose at C3, is found to suppress plant hormone gibberellin (GA) signaling in rice. Scaffold protein RACK1A in the model plant Arabidopsis is implicated in the GA pathway as rack1a knockout mutants show insensitivity to GA in GA-induced seed germination. Using genetic knockout lines and a reporter gene, the functional role of RACK1A in the D-allose pathway was investigated. It was found that the rack1a knockout seeds showed hypersensitivity to D-allose-induced inhibition of seed germination, implicating a role for RACK1A in the D-allose mediated suppression of seed germination. On the other hand, a functional RACK1A in the background of the double knockout mutations in the other two RACK1 isoforms, rack1b/rack1c, showed significant resistance to the D-allose induced inhibition of seed germination. The collective results implicate the RACK1A in the D-allose mediated seed germination inhibition pathway. Elucidation of the rare sugar signaling mechanism will help to advance understanding of this less studied but important cellular signaling pathway.

  10. Development of seismic design method for free standing rack and applicability to Japanese nuclear power plant

    International Nuclear Information System (INIS)

    Takaki, Yu; Taniguchi, Katsuhiko; Kishimoto, Junichi; Iwasaki, Akihisa; Nekomoto, Yoshitsugu; Kuga, Tohru; Kameyama, Masashi

    2017-01-01

    Free standing racks which are not anchored to the pool floor nor walls have never been adopted in Japan. Under an earthquake, behaviors of free standing racks are nonlinear and involve a complex combination of motions (sliding, rocking, and twisting) and impacts between a fuel assembly and the fuel cell walls and between a pit floor and rack pedestals. To predict a seismic response of free standing racks, the seismic analysis requires careful considerations of these complex phenomena (sliding, rocking, and twisting), fluid coupling effects and frictional effects. We carried out seismic experiments on the full-scale rack model in both water and dry conditions and obtained the fundamental data about behavior of free standing racks (sliding, and rocking motions). We have developed the nonlinear dynamic analysis method to predict seismic response of free standing racks utilizing the full-scale test result and verified the analysis evaluation method of free standing rack by comparison between analysis results and experimental data. Furthermore, we applied the seismic design method to the free standing rack in the Japanese nuclear plant (Mihama nuclear power station Unit 3), and verified that the free standing rack was applicable to Japanese nuclear plant. (author)

  11. Mathematical Modelling of Involute Spur Gears Manufactured by Rack Cutter

    Directory of Open Access Journals (Sweden)

    Tufan Gürkan YILMAZ

    2016-05-01

    Full Text Available In this study, mathematical modelling of asymmetric involute spur gears was situated in by Litvin approach. In this context, firstly, mathematical expressions of rack cutter which manufacture asymmetric involute spur gear, then mathematical expression of asymmetric involute spur gear were obtained by using differential geometry, coordinate transformation and gear theory. Mathematical expressions were modelled in MATLAB and output files including points of involute spur gear’s teeth were designed automatically thanks to macros.

  12. Parametric Evaluation of Racking Performance of Platform Timber Framed Walls

    OpenAIRE

    Dhonju, R..; D’Amico, B..; Kermani, A..; Porteous, J..; Zhang, B..

    2017-01-01

    This paper provides a quantitative assessment of the racking performance of partially anchored timber framed walls, based on experimental tests. A total of 17 timber framed wall specimens, constructed from a combination of materials under different load configurations, were tested. The experimental study was designed toexamine the influence of a range of geometrical parameters, such as fastener size and spacings, wall length, arrangement of studs and horizontal members, as well as the effect ...

  13. C-188 Co-60 sources installation and source rack loading optimization processes in a gamma irradiation facility

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Paulo de S.; Vasquez, Pablo A.S., E-mail: psantos@ipen.br, E-mail: pavsalva@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2017-11-01

    Since 2004, the Multipurpose Gamma Facility at the Nuclear and Energy Research Institute has been providing services on radiation processing for disinfection and sterilization of health care and disposable medical products as well to support research studies on modification of physical, chemical and biological properties of several materials. Recently, there was an increment in irradiation of the Cultural Heritages. This facility uses C-188 double-encapsulated radioactive Cobalt-60 sources known as pencils from manufactures outside of country. The activity of the cobalt sources decays into a stable nickel isotope with a half-life around 5.27 years, which means a loss of 12.3% annually. Then, additional pencils of Cobalt-60 are added periodically to the source rack to maintain the required capacity or installed activity of the facility. The manufacturer makes shipping of the radioactive sources inside a high density container type B(U) , by sea. This one involves many administrative, transport and radiation safety procedures. Once in the facility, the container is opened inside a deep pool water to remove the pencils. The required source geometry of the facility is obtained by loading these source pencils into predetermined diagram or positions in source modules and distributing these modules over the source rack of the facility. The dose variation can be reduced placing the higher activity source pencils near the periphery of the source rack. In this work are presented the procedures for perform the boiling leaching tests applied to the container, the Cobalt-60 sources installation, the loading processes and the source rack loading optimization. (author)

  14. Fuzzy sliding mode controller of a pneumatic active isolating system using negative stiffness structure

    International Nuclear Information System (INIS)

    Le, Thanh Danh; Ahn, Kyoung Kwan

    2012-01-01

    A novel active vibration isolation system using negative stiffness structure (active system with NSS) for low excitation frequency ranges (< 5 Hz) is developed successfully. Here, the negative stiffness structure (NSS) is used to minimize the attraction of vibration. Then, the fuzzy sliding mode controller (FSMC) is designed to improve the vibration isolation performance of the active system with NSS. Based on Lyapunov stability theorem, the fuzzy control rules are constructed. Next, the experimental apparatus is built for evaluating the isolation efficiency of the proposed system controlled by the FSMC corresponding to various excitation conditions. In addition, the isolation performance of the active system with NSS, the active system without NSS and the passive the system with NSS is compared. The experimental results confirmed that the active system with NSS gives better isolation efficiency than the active system without NSS and the passive system with NSS in low excitation frequency areas

  15. Amylase activity of a starch degrading bacteria isolated from soil ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-09-17

    Sep 17, 2008 ... was maximum in the temperature range of 50 - 70oC, whereas this temperature range was deleterious for this ... The starch nutrient medium was inoculated with a single isolated .... (1983) high temperature may inactivate the.

  16. Antibacterial activities of lactic acid bacteria isolated from cow ...

    African Journals Online (AJOL)

    Method: Escherichia coli, Klebsiella species (spp) and LAB were isolated from thirty different cow faecal samples and the .... The PCR products were purified and sequenced for the ... their ability to produce bacteriocin-like inhibitory sub-.

  17. Biological activity of some bacterial isolates against soil borne pathogenic fungi

    International Nuclear Information System (INIS)

    Makbol, H.; Refae, R.I.; Eid, H.A.; Mohamed, O.M.

    2011-01-01

    The antagonistic activity of three bacterial isolates namely Micro bacterium terregens, Cellulosimicrobium cellulans and Bacillus amyloliquefaciens was evaluated through direct confrontation method and filtrates culture against the growth of Fusarium solani, Fusarium oxysporum, Rhizoctonia solani and Phytophthra cactorum. All bacterial isolates showed the inhibition of the mycelia growth of the isolated fungi as resulting to confrontation methods except R. solani with C. cellulans that showed no inhibitory effect and energized the low activity with B. amyloliquefaciens. Culture filtrate of different bacterial isolates after different incubation periods revealed that the highest antifungal activity between 3-10 days

  18. Rack Aware Data Placement for Network Consumption in Erasure-Coded Clustered Storage Systems

    Directory of Open Access Journals (Sweden)

    Bilin Shao

    2018-06-01

    Full Text Available The amount of encoded data replication in an erasure-coded clustered storage system has a great impact on the bandwidth consumption and network latency, mostly during data reconstruction. Aimed at the reasons that lead to the excess data transmission between racks, a rack aware data block placement method is proposed. In order to ensure rack-level fault tolerance and reduce the frequency and amount of the cross-rack data transmission during data reconstruction, the method deploys partial data block concentration to store the data blocks of a file in fewer racks. Theoretical analysis and simulation results show that our proposed strategy greatly reduces the frequency and data volume of the cross-rack transmission during data reconstruction. At the same time, it has better performance than the typical random distribution method in terms of network usage and data reconstruction efficiency.

  19. Seismic evaluation of BWR spent fuel storage racks using actual damping by vibration test in water

    International Nuclear Information System (INIS)

    Yamasaki, Hiroto; Iwakura, Shigeyoshi; Imaoka, Tetsuo; Okumura, Kazue; Orita, Syuichi; Namita, Yoshio

    2010-01-01

    Damping value for BWR spent fuel storage racks has been used 1 percent damping, which is applied to welded steel structures in air as defined JEAG4601. However, it is considered that the actual damping is higher than that of the above mentioned, because of its underwater installation. This report shows the actual damping value of the Check Arrayed Rack by vibration test in water and Evaluation by the analysis of rack using actual damping. (author)

  20. Isolation of Lactic Acid Bacteria with High Biological Activity from Local Fermented Dairy Products

    Directory of Open Access Journals (Sweden)

    B. Munkhtsetseg

    2009-12-01

    Full Text Available The thirty-two strains of lactic acid bacteria were isolated from the Mongolian traditional fermented dairy products, among them 25 strains show antimicrobial activity against test microorganisms including Escherichia coli , Staphylococcus aureus , Enterococcus faecalis , Pseudom о nas aeruginosa . Protease sensitivity assay demonstrated that the antimicrobial substances produced by isolates А 23, Т 2 are bacteriocins as their antibacterial activities were eliminated completely after treatment with protease. Identi fi cation of bacteria is being carried out. Among the isolates 22 strains show protease enzyme producing activity. The selected strains isolated from mare’s fermented milk (airag or kumis and yoghurt (tarag show the speci fi c protease activity from 7.9 μ g/ml to 11.9 μ g/ml. The strain T2, isolated from yoghurt exhibited the highest proteolytic activity.

  1. The association between physical activity and social isolation in community-dwelling older adults.

    Science.gov (United States)

    Robins, Lauren M; Hill, Keith D; Finch, Caroline F; Clemson, Lindy; Haines, Terry

    2018-02-01

    Social isolation is an increasing concern in older community-dwelling adults. There is growing need to determine effective interventions addressing social isolation. This study aimed to determine whether a relationship exists between physical activity (recreational and/or household-based) and social isolation. An examination was conducted for whether group- or home-based falls prevention exercise was associated with social isolation. Cross-sectional analysis of telephone survey data was used to investigate relationships between physical activity, health, age, gender, living arrangements, ethnicity and participation in group- or home-based falls prevention exercise on social isolation. Univariable and multivariable ordered logistic regression analyses were conducted. Factors found to be significantly associated with reduced social isolation in multivariable analysis included living with a partner/spouse, reporting better general health, higher levels of household-based physical activity (OR = 1.03, CI = 1.01-1.05) and feeling less downhearted/depressed. Being more socially isolated was associated with symptoms of depression and a diagnosis of congestive heart failure (pseudo R 2 = 0.104). Findings suggest that household-based physical activity is related to social isolation in community-dwelling older adults. Further research is required to determine the nature of this relationship and to investigate the impact of group physical activity interventions on social isolation.

  2. Accident Analysis of High Density Storage Rack for Fresh Fuel Assemblies

    International Nuclear Information System (INIS)

    Jang, K. J.; Lee, M. J.; Jin, H. U.; Park, J. H.; Shin, S. Y.

    2009-01-01

    Recently KONES and KNF have developed the so called suspension-type High Density Storage Rack (HDSR) for fresh fuel assemblies. The USNRC OT position paper specifies that the design of the rack must ensure the functional integrity of the fuel racks under all credible fuel assembly drop events. In this context the functional integrity means the criticality safety. That is to say, the drop events must not bring any danger to the criticality safety of HDSR. This paper shows the results of the analysis carried out to demonstrate the regulatory compliance of the proposed racks under postulated accidental drop events

  3. Effects of poison panel shrinkage and gaps on fuel storage rack reactivity

    International Nuclear Information System (INIS)

    Boyd, W.A.; Mueller, D.E.

    1988-01-01

    Fixed poison panels are used in spent fuel rack designs to increase enrichment limits and reduce cell spacing; therefore, assurances that the maximum rack reactivity will meet the design limit (0.95) throughout the lifetime of the racks depend on the continued effectiveness of the poison with time. Industry data have shown that poison panels will shrink under irradiated conditions. From recent data, however, poison panels have been found to have gaps spanning their width after relatively short operating periods. This paper presents results of studies showing the fuel rack reactivity changes associated with poison panel shrinkage and formation of gaps. The discovery of gaps in the fuel rack poison panels at an operating plant raises concerns regarding the effectiveness of the poison over the lifetime of the fuel racks. Studies performed to evaluate the effect of the poison panel shrinkage on reactivity show that reactivity changes from zero to several percent are possible depending on the initial panel size. Results of recent studies show that some gaps can be accommodated in the fuel rack poison panels at the fuel midplane without causing the fuel rack K eff limit to be exceeded. With worst-case assumptions concerning gap size and the number of panels affected, other actions will likely be required to show that the rack K eff design limit will not be exceeded

  4. Thermal Analysis Evaluation of Spent Fuel Storage Rack for Research Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Sangjin; Oh, Jinho; Kwak, Jinsung; Lee, Jongmin [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2016-10-15

    Spent fuel storage rack is to store spent fuel assemblies. The spent fuel storage rack is submerged in the designated pool for cooling. Due to the condition change of the pool water, the effect of thermal load on spent fuel storage rack must be analyzed and evaluated. In this paper, thermal stress analysis is performed and evaluated on a spent fuel storage rack. For thermal stress evaluation of the spent fuel storage rack, load combinations and allowable criteria in ASME Sec. III NB-3220 are applied. In cases of A-1 and B-1, the same temperature applied on the whole model, thermal stress doesn't occur because there is no constraint about the thermal expansion. The support frame is located on the pool bottom in free standing type and the racks are located in the support frame with enough space. Thermal expansion was considered and reflected in the design of spent fuel storage rack in advance. Thermal stress analysis is performed and evaluated on a spent fuel storage rack with consideration of pool water temperature variation. The thermal analysis including a linear heat transfer and the thermal stress analysis is performed for the racks and support frame and resulted stresses are within allowable criteria.

  5. Manufacturing and Construction of Spent Fuel Storage Rack for Research Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Sangjin; Jung, Kwangsub; Oh, Jinho; Lee, Jongmin [KAERI, Daejeon (Korea, Republic of)

    2016-05-15

    The spent fuel storage rack consists of spent fuel storage racks and support frame. The spent fuel storage racks are installed in the support frame. A spent fuel storage rack consists of frame weldment and storage cell pipe assembly. Storage cell pipe assembly is mounted on the base plate of the frame weldment. The spent fuel storage rack is designed to withstand seismic load and other loads during earthquake. The structural integrity of the spent fuel storage rack is evaluated in accordance with ASME Section III, Subsection NF. Computer Code used for this analysis is ANSYS version 14.0.0. Dead load and seismic load is considered in load condition and hydrodynamic mass is included in the analysis. Design, manufacturing, and construction of the spent fuel storage rack are introduced. The spent fuel storage rack is for storage of spent fuel assemblies. The spent fuel storage rack should be designed, manufactured, and installed with consideration of predicted number of spent fuel assemblies, structural integrity, resistivity to corrosion and radiation, cleaning, and workability.

  6. Seismic response of free standing fuel rack construction to 3-D floor motion

    International Nuclear Information System (INIS)

    Soler, A.I.; Singh, K.

    1983-01-01

    Seismic analysis of free standing submerged racks is complicated by the presence of water and structural non-linearities such as fuel assembly cell impact and floor interface friction. A direct time integration technique has been proposed to analyze this class of structures. Application of the time integration technique on a fourteen degree of freedom lumped mass model of the rack reveals some heretofore unpublished quirks in the structure's behavior. The method of analysis is utilized to compare the seismic response of some representative rack designs. Results show wide differences in the structural response, depending on the fabrication details of racks

  7. Inspection and Analysis of Aluminum Storage Racks in Spent Fuel Storage Basins

    International Nuclear Information System (INIS)

    Howell, J.P.; Nelson, D.Z.

    1998-07-01

    Aluminum-clad spent nuclear fuel is stored in water filled basins at the Savannah River Site awaiting processing or other disposition. After more than 35 years of service underwater, the aluminum storage racks that position the fuel bundles in the basin were replaced. During the removal of the racks from the basin, a failure occurred in one of the racks and the Savannah River Technology Center was asked to investigate. This paper presents results of the failure analysis and provides a discussion of the effects of corrosion on the structural integrity of the storage racks

  8. Studies on amylase activity of an amylolytic bacterium isolated from ...

    African Journals Online (AJOL)

    Diverse microscopic, macroscopic and biochemical analysis of a starch degrading amylolytic bacterial strain isolated from the soil sample of Rajakkamangalam estuary, Kanyakumari district, Tamil Nadu, India, revealed its identity to the genus Bacillus. Maximum growth was observed at 12 h when the bacteria was cultured ...

  9. Terpenylated coumarins as SIRT1 activators isolated from Ailanthus altissima

    DEFF Research Database (Denmark)

    Dao, Trong-Tuan; Tran, Tien-Lam; Kim, Jayeon

    2012-01-01

    Four new terpenylated coumarins (1-4) were isolated from the stem bark of Ailanthus altissima by bioactivity-guided fractionation using an in vitro SIRT1 deacetylation assay. Their structures were identified as (2'R,3'R)-7-(2',3'-dihydroxy-3',7'-dimethylocta-6'-enyloxy)-6,8-dimethoxycoumarin (1),...

  10. Antimicrobial activities of lactic acid bacteria isolated from akamu ...

    African Journals Online (AJOL)

    SAM

    2014-07-16

    Jul 16, 2014 ... culturing on slants of appropriate media and stored at 4°C. Before each experiment ... as frozen culture in MRS broth supplemented with 25% sterile glycerol .... isolates was heated at different temperatures ranging from 40 to. 80°C for .... following treatment at pH 4.0, while complete inactivation occurred at ...

  11. Antifungal activity of plant growth-promoting rhizobacteria isolates ...

    African Journals Online (AJOL)

    Seven plant growth-promoting rhizobacterial (PGPR) strains were isolated from the rhizoplane and rhizosphere of wheat from four different sites of Pakistan. These strains were analyzed for production of indole acetic acid (IAA), phosphorous solublization capability and inhibition of Rhizoctonia solani on rye agar medium.

  12. Cellulolytic activities of wild type fungi isolated from decayed wood ...

    African Journals Online (AJOL)

    Prof. Ogunji

    amongst the fungal isolates while M. mucedo had the least cellulolytic ... across plant taxa, high cellulose content; typically in the range of ... antibiotic mixture made up of distilled water, 50 ml and Erythromycin: 500mg was .... cellulases including β-glucosidase were produced from Penicillium, Aspergillus and Trichoderma ...

  13. Antibacterial activity of chrysophanol isolated from Aloe excelsa ...

    African Journals Online (AJOL)

    AJB SERVER

    identification of Aloin A and Aloe emodium has already been achieved in A. excelsa (Coopoosamy and Magwa,. 2006) Chrysophanol is known to occur in both commercial viable species of aloes (Aloe vera and A. ferox), but has not previously been isolated from A. excelsa. MATERIALS AND METHODS. Measurements.

  14. Antibacterial Activities of Lactic Acid Bacteria Isolated from Selected ...

    African Journals Online (AJOL)

    Members of lactic acid bacteria (LAB) are known probiotics and have been reported to have antimicrobial properties. Although various researchers have documented the isolation of these bacteria from fruits and vegetables, studies on LAB associated with lettuce, cucumber and cabbage are limited and non-existing in ...

  15. Simple and efficient methods for isolation and activity measurement ...

    African Journals Online (AJOL)

    Jane

    2011-06-29

    Jun 29, 2011 ... Key words: Hirudin, thrombin titration method, chromatography, purification. INTRODUCTION. Since recombinant ... Escherichia coli in 1986, intensive research had been .... mixed with 50 µl sample was incubated in 37°C water for 5 min, then 5 µl .... conclusion, the concise and efficient isolation line of the.

  16. Differentiation of enzymatic activity of yeasts and yeast-like microorganisms isolated from various environments

    Directory of Open Access Journals (Sweden)

    Elżbieta Bogusławska-Wąs

    2014-08-01

    Full Text Available The aim of study was to determinate enzymatic activity of yeast-like organisms - Candida lipolytica, Rhodotorula rubra, Trichosporon beigelii, Zygosaccharomyces sp. - isolated from the Szczecin Lagoon and herring salads. We have shown that lipolytic activity was higher than protcolytic for every strain tested. The lowest activity level was found out for amylolytic hydrolases. The results also demonstrated that yeast-like organisms isolated from the Szczecin Lagoon revealed much higher average enzymatic activity compared to tbe same species isolated from herring salads, excepting C. lipolytica.

  17. Carbon Dioxide Dispersion in the Combustion Integrated Rack Simulated Numerically

    Science.gov (United States)

    Wu, Ming-Shin; Ruff, Gary A.

    2004-01-01

    When discharged into an International Space Station (ISS) payload rack, a carbon dioxide (CO2) portable fire extinguisher (PFE) must extinguish a fire by decreasing the oxygen in the rack by 50 percent within 60 sec. The length of time needed for this oxygen reduction throughout the rack and the length of time that the CO2 concentration remains high enough to prevent the fire from reigniting is important when determining the effectiveness of the response and postfire procedures. Furthermore, in the absence of gravity, the local flow velocity can make the difference between a fire that spreads rapidly and one that self-extinguishes after ignition. A numerical simulation of the discharge of CO2 from PFE into the Combustion Integrated Rack (CIR) in microgravity was performed to obtain the local velocity and CO2 concentration. The complicated flow field around the PFE nozzle exits was modeled by sources of equivalent mass and momentum flux at a location downstream of the nozzle. The time for the concentration of CO2 to reach a level that would extinguish a fire anywhere in the rack was determined using the Fire Dynamics Simulator (FDS), a computational fluid dynamics code developed by the National Institute of Standards and Technology specifically to evaluate the development of a fire and smoke transport. The simulation shows that CO2, as well as any smoke and combustion gases produced by a fire, would be discharged into the ISS cabin through the resource utility panel at the bottom of the rack. These simulations will be validated by comparing the results with velocity and CO2 concentration measurements obtained during the fire suppression system verification tests conducted on the CIR in March 2003. Once these numerical simulations are validated, portions of the ISS labs and living areas will be modeled to determine the local flow conditions before, during, and after a fire event. These simulations can yield specific information about how long it takes for smoke and

  18. Semi-active variable stiffness vibration control of vehicle seat suspension using an MR elastomer isolator

    International Nuclear Information System (INIS)

    Du, Haiping; Li, Weihua; Zhang, Nong

    2011-01-01

    This paper presents a study on continuously variable stiffness control of vehicle seat suspension using a magnetorheological elastomer (MRE) isolator. A concept design for an MRE isolator is proposed in the paper and its behavior is experimentally evaluated. An integrated seat suspension model, which includes a quarter-car suspension and a seat suspension with a driver body model, is used to design a sub-optimal H ∞ controller for an active isolator. The desired control force generated by this active isolator is then emulated by the MRE isolator through its continuously variable stiffness property when the actuating condition is met. The vibration control effect of the MRE isolator is evaluated in terms of driver body acceleration responses under both bump and random road conditions. The results show that the proposed control strategy achieves better vibration reduction performance than conventional on–off control

  19. Recent results of seismic isolation study in CRIEPI: Numerical activities

    International Nuclear Information System (INIS)

    Shiojiri, Hiroo; Ishida, Katsuhiko; Yabana, Shurichi; Hirata, Kazuta

    1992-01-01

    Development of detailed numerical models of a bearing and the related isolation system Is necessary for establishing the rational design of the bearing and the system. The developed numerical models should be validated regarding the physical parameters and the basic assumption by comparing the experimental results with the numerical ones. The numerical work being conducted in CRIEPI consists of the following items: (1) Simple modeling of the behavior of the bearings capable of approximating the tests on bearings, and the validation of the model for the bearing by comparing the numerical results adopting the models with the shaking table tests results; (2) Detailed three-dimensional modeling of single bearings with finite-element codes, and the experimental validation of the model; (3)Simple and detailed three-dimensional modeling of isolation buildings and experimental validation

  20. Semi-active control of magnetorheological elastomer base isolation system utilising learning-based inverse model

    Science.gov (United States)

    Gu, Xiaoyu; Yu, Yang; Li, Jianchun; Li, Yancheng

    2017-10-01

    Magnetorheological elastomer (MRE) base isolations have attracted considerable attention over the last two decades thanks to its self-adaptability and high-authority controllability in semi-active control realm. Due to the inherent nonlinearity and hysteresis of the devices, it is challenging to obtain a reasonably complicated mathematical model to describe the inverse dynamics of MRE base isolators and hence to realise control synthesis of the MRE base isolation system. Two aims have been achieved in this paper: i) development of an inverse model for MRE base isolator based on optimal general regression neural network (GRNN); ii) numerical and experimental validation of a real-time semi-active controlled MRE base isolation system utilising LQR controller and GRNN inverse model. The superiority of GRNN inverse model lays in fewer input variables requirement, faster training process and prompt calculation response, which makes it suitable for online training and real-time control. The control system is integrated with a three-storey shear building model and control performance of the MRE base isolation system is compared with bare building, passive-on isolation system and passive-off isolation system. Testing results show that the proposed GRNN inverse model is able to reproduce desired control force accurately and the MRE base isolation system can effectively suppress the structural responses when compared to the passive isolation system.

  1. Impact Analysis for Fuel Assemblies in Spent Fuel Storage Rack

    International Nuclear Information System (INIS)

    Oh, Jinho

    2013-01-01

    The design and structural integrity evaluation of a spent fuel storage rack (SFSR) utilized for storing and protecting the spent fuel assemblies generated during the operation of a reactor are very important in terms of nuclear safety and waste management. The objective of this study is to show the validity of the SFSR design as well as fuel assembly through a structural integrity evaluation based on a numerical analysis. In particular, a dynamic time history analysis considering the gaps between the fuel assemblies and the walls of the storage cell pipes in the SFSR was performed to check the structural integrity of the fuel assembly and storage cell pipe

  2. Plasmonic rack-and-pinion gear with chiral metasurface

    Science.gov (United States)

    Gorodetski, Yuri; Karabchevsky, Alina

    2016-04-01

    The effect of circularly polarized beaming excited by traveling surface plasmons, via chiral metasurface is experimentally studied. Here we show that the propagation direction of the plasmonic wave, evanescently excited on the thin gold film affects the handedness of the scattered beam polarization. Nanostructured metasurface leads to excitation of localized plasmonic modes whose relative spatial orientation induces overall spin-orbit interaction. This effect is analogical to the rack-and-pinion gear: the rotational motion into the linear motion converter. From the practical point of view, the observed effect can be utilized in integrated optical circuits for communication systems, cyber security and sensing.

  3. Impact Analysis for Fuel Assemblies in Spent Fuel Storage Rack

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Jinho [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2013-07-01

    The design and structural integrity evaluation of a spent fuel storage rack (SFSR) utilized for storing and protecting the spent fuel assemblies generated during the operation of a reactor are very important in terms of nuclear safety and waste management. The objective of this study is to show the validity of the SFSR design as well as fuel assembly through a structural integrity evaluation based on a numerical analysis. In particular, a dynamic time history analysis considering the gaps between the fuel assemblies and the walls of the storage cell pipes in the SFSR was performed to check the structural integrity of the fuel assembly and storage cell pipe.

  4. Nuclear fuel rack lateral support and preload device

    International Nuclear Information System (INIS)

    Knight, C.B.

    1979-01-01

    A lateral support device for a nuclear fuel rack is described. It is vertically positioned within a walled enclosure which provides a preload to maintain effective support. The device includes a scissors-jack which is mounted so that the drive screw is vertical. Upon rotation of the drive screw the jack expands horizontally, seating a load pad against a vertical wall. The load pad is affixed to the jack by an arm having a stack of spring washers which compress between the pad and the jack, thereby applying a selected preload

  5. Isolation, Identification, and Xanthine Oxidase Inhibition Activity of Alkaloid Compound from Peperomia pellucida

    Science.gov (United States)

    Fachriyah, E.; Ghifari, M. A.; Anam, K.

    2018-04-01

    The research of the isolation and xanthine oxidation inhibition activity of alkaloid compound from Peperomia pellucida has been carried out. Alkaloid extract is isolated by column chromatography and preparative TLC. Alkaloid isolate is identified spectroscopically by UV-Vis spectrophotometer, FT-IR, and LC-MS/MS. Xanthine oxidase inhibition activity is carried out by in vitro assay. The result showed that the alkaloid isolated probably has piperidine basic structure. The alkaloid isolate has N-H, C-H, C = C, C = O, C-N, C-O-C groups and the aromatic ring. The IC50 values of ethanol and alkaloid extract are 71.6658 ppm and 76.3318 ppm, respectively. Alkaloid extract of Peperomia pellucida showed higher activity than ethanol extract.

  6. Halotolerant ability and α-amylase activity of some saltwater fungal isolates

    NARCIS (Netherlands)

    Niknejad, F.; Moshfegh, M.; Najafzadeh, M.J.; Houbraken, J.; Rezaei, S.; Zarrini, G.; Faramarzi, M.A.; Nafissi-Varcheh, N.

    2013-01-01

    Four halotolerant fungal isolates originating from the saltwater Lake Urmia in Iran were selected during a screening program for salt resistance and α-amylase activity. The isolates were identified based on sequencing the ITS region and a part of the β-tubulin gene, as Penicillium chrysogenum

  7. Isolation of Lactic Acid Bacteria with High Biological Activity from Local Fermented Dairy Products

    OpenAIRE

    B. Munkhtsetseg; M. Margad-Erdene; B. Batjargal

    2009-01-01

    The thirty-two strains of lactic acid bacteria were isolated from the Mongolian traditional fermented dairy products, among them 25 strains show antimicrobial activity against test microorganisms including Escherichia coli , Staphylococcus aureus , Enterococcus faecalis , Pseudom о nas aeruginosa . Protease sensitivity assay demonstrated that the antimicrobial substances produced by isolates А 23, Т 2 are bacterio...

  8. Improved method to demonstrate the structural integrity of high density fuel storage racks

    International Nuclear Information System (INIS)

    Hinderks, M.; Ungoreit, H.; Kremer, G.

    2001-01-01

    Reracking of existing fuel pools to the maximum extent is desirable from an economical point of view. This goal can be achieved by minimizing the gaps between the spent fuel storage racks. Since the rack design is aimed at enabling consolidated fuel rod storage, additional requirements arise with respect to the design and the structural analysis. The loads resulting from seismic events are decisive for the structural analysis and require a specially detailed and in-depth analysis for high seismic loads. The verification of structural integrity and functionality is performed in two phases. In the first phase the motional behavior of single racks, rows of racks and, where required, of all racks in the pool is simulated by excitation with displacement time histories under consideration of the fluid-structure interaction (FSI). The displacements from these simulations are evaluated, while the loads are utilized as input data for the structural analysis of the racks and the pool floor. The structural analyses for the racks comprise substantially stress analyses for base material and welds as well as stability analyses for the support channels and the rack outside walls. The analyses are performed in accordance with the specified codes and standards

  9. 16 CFR Figure 3 to Part 1610 - Specimen Holder Supported in Specimen Rack

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Specimen Holder Supported in Specimen Rack 3 Figure 3 to Part 1610 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FLAMMABLE FABRICS ACT... Holder Supported in Specimen Rack ER25MR08.002 ...

  10. 40 CFR Table 3 to Subpart Eeee of... - Operating Limits-High Throughput Transfer Racks

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Operating Limits-High Throughput Transfer Racks 3 Table 3 to Subpart EEEE of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION... Throughput Transfer Racks As stated in § 63.2346(e), you must comply with the operating limits for existing...

  11. The optimization of spent fuel assembly storage racks in nuclear power plants

    International Nuclear Information System (INIS)

    Wang Yan

    2005-01-01

    This paper gives an evaluation of the spent fuel assembly storage racks in the nuclear power plants at home and abroad, focusing on the characteristics of the high density storage racks and the aseismatic design. It mainly discusses structures and characteristics of the spent fuel assembly storage racks in the Qinshan nuclear power phase II project. Concluding the crucial technical difficulties of the high density spent fuel assembly storage racks: the neutron-absorbing materials, the structural aseismatic design technology and the security analysis technology, this paper firstly generalizes several important neutron-absorbing materials, then introduces the evolution of the aseismatic design of the spent fuel assembly storage racks . In the last part, it describes the advanced aseismatic analysis technology in the Qinshan nuclear power phase II project. Through calculation and analysis for such storage racks, the author concludes several main factors that could have an influence on the aseismatic performance and thus gives the key points and methods for designing the optimal racks and provides some references for the design of advanced spent fuel assembly storage racks in the future. (authors)

  12. The fabrication of plastic cages for suspension in mass air flow racks.

    Science.gov (United States)

    Nielsen, F H; Bailey, B

    1979-08-01

    A cage for suspension in mass air flow racks was constructed of plastic and used to house rats. Little or no difficulty was encountered with the mass air flow rack-suspended cage system during the 4 years it was used for the study of trace elements.

  13. Isolation and characterization of pigmented bacteria showing antimicrobial activity from Malaysian marine environment

    Directory of Open Access Journals (Sweden)

    Ahmad, A.

    2013-01-01

    Full Text Available Aims: Natural products play a prominent role in the discovery of leads for the development of drugs in the treatment ofhuman diseases. Much of nature remains to be explored, especially marine and microbial environments.Methodology and results: Fifty-five pigmented marine bacteria were isolated from sponges, seawater, mangrovesediment, sea cucumber and mussel from different coastal area of Malaysia. The antimicrobial activities of thesebacteria were investigated by disk diffusion method against pathogenic bacteria. Out of 55 isolates, 18 isolates exhibitedantimicrobial activity, which based on morphological characterization, 53% of them were Gram positive and 47% wereGram negative. All active isolates were able to tolerate more than 4% NaCl in the nutrient agar medium that indicatedthey were autochthonous to marine environment and moderate salt tolerant in nature. Molecular identification of isolatesby the strong antimicrobial activities indicates that isolates WPRA3 (JX020764 and SM11-3j belong to genus Serratiaand isolate SDPM1 (JQ083392 belongs to genus Zooshikella.Conclusion, significance and impact of study: The results of present study revealed that the active isolates arepotential producer of antimicrobial secondary metabolites and might be utilized as drug candidate.

  14. Evaluation of Antimicrobial Activity of Bacillus Strains Isolated from Various Resources

    Directory of Open Access Journals (Sweden)

    Mohsen Golnari Maranni

    2017-02-01

    Full Text Available Abstract Background: Prevalence extension of antibiotic resistant bacteria has raised concerns about control of infections especially nosocomial infections. Many attempts have been done to replace antibiotics or limit their use. The use of antimicrobial agents produced by bacteria as antibiotic replacement has been promising in recent years. The goal of this study was to isolate Bacillus strains and evaluate their antimicrobial activity against some standard pathogens and clinical antibiotic resistant strains. Materials and Methods: In the present study, Bacillus strains were isolated from various resources and identified by 16S rDNA PCR method. Then, the phylogenetic tree of the isolates was constructed and antimicrobial activity of the isolates was investigated against some standard pathogens and clinical antibiotic resistant strains using spotting and well diffusion methods. Results: Eight Bacillus strains were isolated from 15 different samples. Based on the molecular identification, the isolates were identified as B.pumilus, B.coagulans, B.licheniformis, B.endophitycus and B.amiloliquefaciens. The results showed that isolates have antimicrobial activity against meticilin-resistant Staphylococcus aureus, vancomycin resistant enterococci, Klebsiella, Acinetobacter, Salmonella, Shigella, Listeria, Streptococcus and Escherichia coli. Conclusion: In this study, isolated Bacillus strains produced antimicrobial agents against pathogens and antibiotic resistant strains and inhibited their growth.

  15. Antimicrobial Activity of Cell Free Supernatant of Irradiated Lactic Acid Bacteria Isolates

    International Nuclear Information System (INIS)

    Abdelaleem, M.A.; AL-Hagar, O.E.Aa.

    2015-01-01

    Attempts were made to isolate bio preservatives using food wastes with no value and low cost. Whey is the raw material achieved that value. Whey and many other food wastes are used in our study to isolate Lactic acid bacteria (LAB). Cell free supernatants (CFS) of isolates are used to evaluate their antimicrobial activity against indicator pathogenic bacterial strains. CFS-9 isolate from whey has the highest inhibitory activity compared to all other isolates. The inhibitory activity of CFS-9, Nisin (400 IU / ml) and the standard Lactococcus Lactis Subsp. Lactis ATCC 11454 (Lacto) were determined. Furthermore, isolate-9 and Lacto strains were exposed to irradiation at different doses. The inhibition zones of; control isolate-9 (non-irradiated) showed the highest values against all indicator strains, CFS of irradiated Lacto at dose 250 Gy was the highest value against Bacillus cereus and Escherichia coli compared to other irradiation treatments, CFS of irradiated Lacto at dose 100 Gy was the highest value against Staph aureus, while the inhibition zone was in the highest value in CFS of irradiated Lacto at dose 500 Gy against Salmonella typhimurium. Nisin (400 IU / ml) was significantly higher than all CFS of irradiated isolate-9 while, the inhibition zones of all CFS-Lacto (irradiated and nonirradiated) are better and higher than nisin-400

  16. The role of active efflux in antibiotic - resistance of clinical isolates of Helicobacter pylori

    Directory of Open Access Journals (Sweden)

    Falsafi T

    2009-01-01

    Full Text Available Purpose: In gram-negative bacteria, active efflux pumps that excrete drugs can confer resistance to antibiotics however, in Helicobacter pylori this role is not well established. The purpose of this study is to evaluate the role of active efflux in resistance of H. pylori isolates to antibiotics. Materials and Methods: Twelve multiple antibiotic resistant (MAR isolates resistant to at least four antibiotics, including β-lactams, metronidazole, tetracycline, erythromycin, and ciprofloxacin; three resistant to only β-lactams, and two hyper-susceptible isolates, were obtained from screening of 96 clinical isolates of H. pylori . Their minimal inhibitory concentrations (MICs for antibiotics and ethidium-bromide (EtBr were compared in the presence- and absence of a proton-conductor, carbonyl cyanide-m chlorophenyl-hydrazone (CCCP using agar-dilution and disc diffusion. Drug accumulation studies for EtBr and antibiotics were assessed in the presence and absence of CCCP using spectrofluorometry. Results: MIC of EtBr for eight MAR-isolates was decreased two- to four-folds in the presence of CCCP, of which five showed reduced MICs for β-lactam, metronidazole, tetracycline, and ciprofloxacin with CCCP. Accumulation of EtBr by the MAR-isolates was rapid and not dependant on the pattern of multiple resistance. Antibiotic accumulation assay confirmed the presence of energy-dependant efflux of β-lactam, metronidazole, tetracycline, and ciprofloxacin, but no erythromycin in five MAR isolates. Energy-dependant efflux of EtBr or antibiotics was not observed for four MAR-isolates, and three isolates were resistant only to β-lactams. Conclusion: Energy-dependant efflux plays a role in the resistance of H. pylori clinical isolates to structurally unrelated antibiotics in a broadly specific multidrug efflux manner. Difference in the efflux potential of MAR isolates may be related to the presence or absence of functional efflux-pumps in diverse H. pylori

  17. Isolation of Mucorales from processed maize (Zea mays L.) and screening for protease activity

    OpenAIRE

    de Azevedo Santiago, Andr? Luiz Cabral Monteiro; de Souza Motta, Cristina Maria

    2008-01-01

    Mucorales were isolated from maize flour, corn meal and cooked cornflakes using surface and depth plate methods. Rhizopus oryzae, Circinella muscae, Mucor subtilissimus, Mucor hiemalis f. hiemalis, Syncephalastrum racemosum, Rhizopus microsporus var. chinensis and Absidia cylindrospora showed protease activity.

  18. Studies on Anti-Depressant Activity of Four Flavonoids Isolated from ...

    African Journals Online (AJOL)

    glucose and quercetin-3-O-β-D-glucose isolated from Apocynum venetum Linn. ... may be due to increased NE, DA and 5-HT and reduced 5-HT metabolism. ..... monoaminergic neuronal activity. Biochem. Pharmacol 1985; 34: 1127-1131. 30.

  19. Tests of biological activity of metabolites from Penicillium expansum (Link Thom various isolates

    Directory of Open Access Journals (Sweden)

    Halina Borecka

    2013-12-01

    Full Text Available Aqrobacterium tumefaciens and cucumber, mustard and linseeds were compared as test organisms for evaluation of the biological activity of patulin. It was found that the reaction of cucumber seeds and linseed to the patulin concentrations was more pronounced than that of mustard and Aqrobacterium tumefaciens. The activity of metabolites produced by Penicillium expansum was investigated with the use of cucumber seeds. As measure of activity served the percentage of radicule growth inhibition was compared with the growth in control seeds. The biological activity of the metabolites was specific for the isolates, those from apples being more active. Thirty two isolates from pears and 34 from apples were examined.

  20. Hippocampal theta activity in the acute cerveau isolé cat.

    Science.gov (United States)

    Gottesmann, C; Zernicki, B; Gandolfo, G

    1981-01-01

    In three cerveau isole cats, cortical and hippocampal EEG activity were recorded. In the cortical records, spindles alternated with low-voltage activity, whereas theta activity dominated in the hippocampus. The amount and frequency of theta were similar to those described previously for the pretrigeminal cat. In confirmation of previous results on rats, although cortical EEG activity differs in cerveau isole cat and pretrigeminal cat, both preparations show domination of theta activity in the hippocampus. It is concluded that the mesencephalic transection eliminates inhibitory effects from the lower brainstem on generators of the theta rhythm.

  1. Active Low-frequency Vertical Vibration Isolation System for Precision Measurements

    Institute of Scientific and Technical Information of China (English)

    WU Kang; LI Gang; HU Hua; WANG Lijun

    2017-01-01

    Low-frequency vertical vibration isolation systems play important roles in precision measurements to reduce seismic and environmental vibration noise.Several types of active vibration isolation systems have been developed.However,few researches focus on how to optimize the test mass install position in order to improve the vibration transmissibility.An active low-frequency vertical vibration isolation system based on an earlier instrument,the Super Spring,is designed and implemented.The system,which is simple and compact,consists of two stages:a parallelogram-shaped linkage to ensure vertical motion,and a simple spring-mass system.The theoretical analysis of the vibration isolation system is presented,including terms erroneously ignored before.By carefully choosing the mechanical parameters according to the above analysis and using feedback control,the resonance frequency of the system is reduced from 2.3 to 0.03 Hz,a reduction by a factor of more than 75.The vibration isolation system is installed as an inertial reference in an absolute gravimeter,where it improved the scatter of the absolute gravity values by a factor of 5.The experimental results verifies the improved performance of the isolation system,making it particularly suitable for precision experiments.The improved vertical vibration isolation system can be used as a prototype for designing high-performance active vertical isolation systems.An improved theoretical model of this active vibration isolation system with beam-pivot configuration is proposed,providing fundamental guidelines for vibration isolator design and assembling.

  2. Characterization of Multilayer Piezoelectric Actuators for Use in Active Isolation Mounts

    Science.gov (United States)

    Wise, Stephanie A.; Hooker, Matthew W.

    1997-01-01

    Active mounts are desirable for isolating spacecraft science instruments from on-board vibrational sources such as motors and release mechanisms. Such active isolation mounts typically employ multilayer piezoelectric actuators to cancel these vibrational disturbances. The actuators selected for spacecraft systems must consume minimal power while exhibiting displacements of 5 to 10 micron under load. This report describes a study that compares the power consumption, displacement, and load characteristics of four commercially available multilayer piezoelectric actuators. The results of this study indicate that commercially available actuators exist that meet or exceed the design requirements used in spacecraft isolation mounts.

  3. Hemolytic and urease activities in vibrios isolated from fresh and frozen oysters

    Directory of Open Access Journals (Sweden)

    Renata Albuquerque Costa

    2013-01-01

    Full Text Available INTRODUCTION: The present study aimed to survey the Vibrio microbiota of oysters (Crassostrea rhizophorae obtained from restaurants in Fortaleza, State of Ceará, Brazil, and to identify virulence factors. METHODS: The isolated vibrios were submitted to biochemical identification and were tested for hemolytic and urease activities. RESULTS: The isolated strains belonged to 13 species, with predominance of Vibrio mimicus. Of the strain isolates only from fresh samples, 20.5% and 2.8% showed hemolytic and urease activities, respectively. CONCLUSIONS: The findings support the little-publicized claim that Vibrio species other than V. parahaemolyticus and V. vulnificus can represent a health risk to public health.

  4. 40 CFR Table 5 to Subpart Ffff of... - Emission Limits and Work Practice Standards for Transfer Racks

    Science.gov (United States)

    2010-07-01

    ... Standards for Transfer Racks 5 Table 5 to Subpart FFFF of Part 63 Protection of Environment ENVIRONMENTAL... Limits and Work Practice Standards for Transfer Racks As required in § 63.2475, you must meet each emission limit and work practice standard in the following table that applies to your transfer racks: For...

  5. 77 FR 82 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Extension of...

    Science.gov (United States)

    2012-01-03

    ... Shelving and Racks From the People's Republic of China: Extension of Time Limit for the Final Results of... administrative review of the countervailing duty order on certain kitchen appliance shelving and racks from the... Kitchen Appliance Shelving and Racks From the People's Republic of China: Preliminary Results of the...

  6. 26 CFR 48.4081-3 - Taxable fuel; taxable events other than removal at the terminal rack.

    Science.gov (United States)

    2010-04-01

    ... at the terminal rack. 48.4081-3 Section 48.4081-3 Internal Revenue INTERNAL REVENUE SERVICE...; taxable events other than removal at the terminal rack. (a) Overview. Although tax is imposed when taxable fuel is removed from the terminal at the rack, tax also is imposed in certain other situations...

  7. 78 FR 21594 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Final Results...

    Science.gov (United States)

    2013-04-11

    ... Shelving and Racks From the People's Republic of China: Final Results of Countervailing Duty Administrative... countervailing duty (``CVD'') order on certain kitchen appliance shelving and racks from the People's Republic of...\\ See Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Countervailing...

  8. 78 FR 5414 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China; 2010-2011...

    Science.gov (United States)

    2013-01-25

    ... Shelving and Racks From the People's Republic of China; 2010-2011; Final Results of Antidumping Duty... of the antidumping duty order on certain kitchen appliance shelving and racks from the People's... September 1, 2010, through August 31, 2011. \\1\\ See Certain Kitchen Appliance Shelving and Racks From the...

  9. 78 FR 78815 - Certain Kitchen Appliance Shelving and Racks from the People's Republic of China: Rescission of...

    Science.gov (United States)

    2013-12-27

    ... Shelving and Racks from the People's Republic of China: Rescission of Antidumping Duty Administrative... administrative review of the antidumping duty order on certain kitchen appliance shelving and racks from the... on certain kitchen appliance shelving and racks from the PRC.\\1\\ On September 30, 2013, the...

  10. 77 FR 22559 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Extension of...

    Science.gov (United States)

    2012-04-16

    ... Shelving and Racks From the People's Republic of China: Extension of Time Limit for the Preliminary Results... administrative review of the antidumping duty order on certain kitchen appliance shelving and racks from the... Appliance Shelving and Racks from the People's Republic of China: Selection of an Additional Mandatory...

  11. 76 FR 20950 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Extension of...

    Science.gov (United States)

    2011-04-14

    ... Shelving and Racks From the People's Republic of China: Extension of Time Limits for the Preliminary... appliance shelving and racks from the People's Republic of China (``PRC'') for the period March 5, 2009... Racks from the People's Republic of China,'' dated January 20, 2011. The Department sent its antidumping...

  12. 77 FR 21734 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Final Results...

    Science.gov (United States)

    2012-04-11

    ... Shelving and Racks From the People's Republic of China: Final Results and Partial Rescission of First... duty order on certain kitchen appliance shelving and racks from the People's Republic of China (``PRC... Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Preliminary Results of...

  13. 76 FR 27990 - Certain Kitchen Shelving and Racks From the People's Republic of China: Extension of Time Limit...

    Science.gov (United States)

    2011-05-13

    ... Racks From the People's Republic of China: Extension of Time Limit for Preliminary Results of... countervailing duty order on certain kitchen appliance shelving and racks from the People's Republic of China... the Countervailing Duty Administrative Review of Certain Kitchen Appliance Shelving and Racks from the...

  14. Glycogen synthase activation by sugars in isolated hepatocytes.

    Science.gov (United States)

    Ciudad, C J; Carabaza, A; Bosch, F; Gòmez I Foix, A M; Guinovart, J J

    1988-07-01

    We have investigated the activation by sugars of glycogen synthase in relation to (i) phosphorylase a activity and (ii) changes in the intracellular concentration of glucose 6-phosphate and adenine nucleotides. All the sugars tested in this work present the common denominator of activating glycogen synthase. On the other hand, phosphorylase a activity is decreased by mannose and glucose, unchanged by galactose and xylitol, and increased by tagatose, glyceraldehyde, and fructose. Dihydroxyacetone exerts a biphasic effect on phosphorylase. These findings provide additional evidence proving that glycogen synthase can be activated regardless of the levels of phosphorylase a, clearly establishing that a nonsequential mechanism for the activation of glycogen synthase occurs in liver cells. The glycogen synthase activation state is related to the concentrations of glucose 6-phosphate and adenine nucleotides. In this respect, tagatose, glyceraldehyde, and fructose deplete ATP and increase AMP contents, whereas glucose, mannose, galactose, xylitol, and dihydroxyacetone do not alter the concentration of these nucleotides. In addition, all these sugars, except glyceraldehyde, increase the intracellular content of glucose 6-phosphate. The activation of glycogen synthase by sugars is reflected in decreases on both kinetic constants of the enzyme, M0.5 (for glucose 6-phosphate) and S0.5 (for UDP-glucose). We propose that hepatocyte glycogen synthase is activated by monosaccharides by a mechanism triggered by changes in glucose 6-phosphate and adenine nucleotide concentrations which have been described to modify glycogen synthase phosphatase activity. This mechanism represents a metabolite control of the sugar-induced activation of hepatocyte glycogen synthase.

  15. Thermostable 𝜶-Amylase Activity from Thermophilic Bacteria Isolated from Bora Hot Spring, Central Sulawesi

    Science.gov (United States)

    Gazali, F. M.; Suwastika, I. N.

    2018-03-01

    α-Amylase is one of the most important enzyme in biotechnology field, especially in industrial application. Thermostability of α-Amylase produced by thermophilic bacteria improves industrial process of starch degradation in starch industry. The present study were concerned to the characterization of α-Amylase activity from indigenous thermophilic bacteria isolated from Bora hot spring, Central Sulawesi. There were 18 isolates which had successfully isolated from 90°C sediment samples of Bora hot spring and 13 of them showed amylolytic activity. The α-Amylase activity was measured qualitatively at starch agar and quantitatively based on DNS (3,5-Dinitrosalicylic acid) methods, using maltose as standard solution. Two isolates (out of 13 amylolytic bacteria), BR 002 and BR 015 showed amylolytic index of 0.8 mm and 0.5 mm respectively, after being incubated at 55°C in the 0.002% Starch Agar Medium. The α-Amylase activity was further characterized quantitatively which includes the optimum condition of pH and temperature of α-Amylase crude enzyme from each isolate. To our knowledge, this is the first report on isolation and characterization of a thermostable α-Amylase from thermophilic bacteria isolated from Central Sulawesi particularly from Bora hot spring.

  16. Isolation of a substance activating foot formation in hydra

    DEFF Research Database (Denmark)

    Grimmelikhuijzen, C J; Schaller, H C

    1977-01-01

    -forming potential of the tissue (2) It does not accelerate head regeneration, nor do the head factors of hydra discovered by Schaller (1973) and Berking (1977) accelerate foot regeneration. We propose that the foot-activating substance is a morphogen responsible for foot formation in hydra. The foot activator can...

  17. [The in vitro antifungal activities of fluconazole against pathogenic yeasts recently isolated from clinical specimens].

    Science.gov (United States)

    Yamaguchi, H; Igari, J; Kume, H; Abe, M; Oguri, T; Kanno, H; Kawakami, S; Okuzumi, K; Fukayama, M; Ito, A; Kawata, K; Uchida, K

    1997-09-01

    The emergence of Candida albicans resistance to azole antifungal agents have been reported in the U. S. and Europe. We examined the in vitro antifungal activities of fluconazole against clinical isolates collected by seven investigators in three years to examine if a tendency existed toward the development of azole-resistance among fungal isolates in Japan. The following results were obtained: 1. Sensitivities to fluconazole (FLCZ) were determined for yeast-like fungi, including 113 strains isolated in 1993, 149 strains isolated in 1994 and 205 strains isolated in 1995. No significant differences in sensitivities in the three years were detected. 2. Minimum inhibitory concentrations of FLCZ were 0.1-0.78 microgram/ml for C. albicans and 3.13-25 micrograms/ml for C. glabrata. Strains with 25 micrograms/ml of FLCZ's MIC were detected; two strains of C. krusei and one strain each of C. krusei, Trichospron beigelii and Hansenula anomala. No strains with higher than 50 micrograms/ml MIC of FLCZ were detected. 3. In vitro activities of FLCZ were compared between clinical strains isolated between 1993 and 1995 and clinical strains isolated before the marketing of FLCZ (up to December 1987) or clinical yeasts isolated between 1991 and 1992. No significant differences were observed, suggesting that no tendency existed toward azole resistance among fungal strains examined.

  18. Characterization and lytic activity of methicillin-resistant Staphylococcus aureus(MRSA phages isolated from NICU

    Directory of Open Access Journals (Sweden)

    Golnar Rahimzadeh

    2016-06-01

    Full Text Available Background Methicillin-resistant Staphylococcus aureus (MRSA is a well-known pathogen that causes serious diseases in humans. As part of the efforts to control this pathogen, an isolated bacteriophage, Siphoviridae, which specifically targets Methicillin-resistant Staphylococcus aureus (MRSA, was characterized. Aims The objective of this study was to characterize of a virulent bacteriophage (Siphoviridae isolated from a NICU bathroom sink. Methods The MRSA strain was isolated from patient blood. The isolated strain was confirmed as MRSA using conventional methods. Phages were isolated from a NICU bathroom sink and activity was lytic as determined by spot test. Titer phage lysate was measured by the Double Layer Agar (DLA technique. The morphology was found with electron microscopy. The single-step growth curve was plotted. Results Electron microscopy showed the phage as a member of the family Siphoviridae, serogroup A and F. The isolated phage was capable of lytic activity against methicillin-resistant Staphylococcus aureus (MRSA strain as shown by spot test. By DLA, the titre of the phages was determined to be 10×108PFU/ml. The single-step growth curve showed that the latent period of the isolated bacteriophage was 30 min and the total number of viable progeny per infected host, burst size, was 2600 PFU/infected host. Conclusion In this study, two phages were isolated and characterized from a NICU bathroom sink, from the Siphoviridae family, which specifically targetsmethicillin-resistant Staphylococcus aureus (MRSA.

  19. Isolation and characterization of aerobic microorganisms with cellulolytic activity in the gut of endogeic earthworms.

    Science.gov (United States)

    Fujii, Katsuhiko; Ikeda, Kana; Yoshida, Seo

    2012-09-01

    The ability of earthworms to decompose lignocellulose involves the assistance of microorganisms in their digestive system. While many studies have revealed a diverse microbiota in the earthworm gut, including aerobic and anaerobic microorganisms, it remains unclear which of these species contribute to lignocellulose digestion. In this study, aerobic microorganisms with cellulolytic activity isolated from the gut of two endogeic earthworms, Amynthas heteropoda (Megascolecidae) and Eisenia fetida (Lumbricidae) were isolated by solid culture of gut homogenates using filter paper as a carbon source. A total of 48 strains, including four bacterial and four fungal genera, were isolated from two earthworm species. Characterization of these strains using enzyme assays showed that the most representative ones had exocellulase and xylanase activities, while some had weak laccase activity. These findings suggest that earthworms digest lignocellulose by exploiting microbial exocellulase and xylanase besides their own endocellulase. Phylogenetic analysis showed that among the cellulolytic isolates in both earthworm species Burkholderia and Chaetomium were the dominant bacterial and fungal members.

  20. Antibacterial activity of garlic and lime on isolates of extracted ...

    African Journals Online (AJOL)

    USER

    2010-05-24

    May 24, 2010 ... essential ingredient in the preparation of most herbal ... The antimicrobial activity of the volatile oils of tangerine ... atherosclerosis (Durak et al., 2002), as well as high blood pressure and cancer (Nishino, 1990; Imai, 1994). As.

  1. Antimicrobial activities of lactic acid bacteria isolated from akamu ...

    African Journals Online (AJOL)

    The partially purified inhibitory compounds were screened by agar spot assay method for antagonistic ... The partially purified compounds exhibited strong activity against ... Keywords: Bacteriocins, lactic acid bacteria (LAB), target organisms, ...

  2. activity guided isolation of chemical constituents from the ...

    African Journals Online (AJOL)

    Acer truncatum Bunge by high-speed counter-current chromatography. J. Chromatogr. A. 2005, 1070, 211–214. 11. Morton, J.J.; Malone, M.H. Evaluation of vulneray activity by an open wound procedure in rats. Arch.Int. Pharmacodyn. Ther. 1972, 196, 117–126. 12. Dash, G.K.; Murthy, P.N. Studies on wound healing activity ...

  3. Plant oligoadenylates: enzymatic synthesis, isolation, and biological activities

    International Nuclear Information System (INIS)

    Devash, Y.; Reichman, M.; Sela, I.; Reichenbach, N.L.; Suhadolnik, R.J.

    1985-01-01

    An enzyme that converts [ 3 H, 32 P]ATP, with a 3 H: 32 P ratio of 1:1, to oligoadenylates with the same 3 H: 32 P ratio was increased in plants following treatment with human leukocyte interferon or plant antiviral factor or inoculation with tobacco mosaic virus. The enzyme was extracted from tobacco leaves, callus tissue cultures, or cell suspension cultures. The enzyme, a putative plant oligoadenylate synthetase, was immobilized on poly(rI) . poly(rC)-agarose columns and converted ATP into plant oligoadenylates. These oligoadenylates were displaced from DEAE-cellulose columns with 350 mM KCl buffer, dialyzed, and further purified by high-performance liquid chromatography (HPLC) and DEAE-cellulose gradient chromatography. In all steps of purification, the ratio of 3 H: 32 P in the oligoadenylates remained 1:1. The plant oligoadenylates isolated by displacement with 350 mM KCl had a molecular weight greater than 1000. The plant oligoadenylates had charges of 5- and 6-. HPLC resolved five peaks, three of which inhibited protein synthesis in reticulocyte and wheat germ systems. Partial structural elucidation of the plant oligoadenylates has been determined by enzymatic and chemical treatments. An adenylate with a 3',5'-phosphodiester and/or a pyrophosphoryl linkage with either 3'- or 5'-terminal phosphates is postulated on the basis of treatment of the oligoadenylates with T2 RNase, snake venom phosphodiesterase, and bacterial alkaline phosphatase and acid and alkaline hydrolyses. The plant oligoadenylates at 8 X 10(-7) M inhibit protein synthesis by 75% in lysates from rabbit reticulocytes and 45% in wheat germ cell-free systems

  4. Method of assembling spent nuclear fuel storage rack

    International Nuclear Information System (INIS)

    Igarashi, Ryokichi; Hasegawa, Hidenobu.

    1982-01-01

    Purpose: To improve the safety of a spent fuel storage rack by stably installing the spent fuel in a pool without using supporting beams. Constitution: A restricted unit is composed of a plurality of spuare cylinders. A plurality of such restricted units are aligned in a direction perpendicularly to the arraying direction of the cylinders in the respective restricted units, are coupled with long connecting plates, and are fixed by welding on a common small base, thereby forming a restricted body. According to such assembling method, a plurality of restricted bodies are connected in a direction that the respective restricted bodies are readily overturned, and are secured to the common base. Accordingly, the restricted bodies can be stably installed in a pool without using supporting beams as the conventional one. (Sekiya, K.)

  5. Fixing Trailer Hitch for Roof Rack of Cargos

    Directory of Open Access Journals (Sweden)

    Darina Matisková

    2017-02-01

    Full Text Available This article presents a new technical solution of fixing trailer hitch which serves as a connection between single track vehicle and cargo, mainly light trailer. They are manufactured from steel girder and nets which are constructed by point welding. They are designed to indicate the necessary solidity and the required load bearing capacity of the cargo. The application of roof racks is universal and these products are in great demand in the field of game management. There are a lot of trailer hitches which the manufacturers tailor to customers´ demands. The stated technical solution is subject of published industrial utility model at the Industrial property office of the Slovak republic.

  6. Time-dependent changes in rat lymphocyte activity in response to isolation

    International Nuclear Information System (INIS)

    Jessop, J.J.; Bayer, B.M.

    1986-01-01

    The authors have found that isolation of rats, previously adapted to group-housing conditions, resulted in time-dependent changes in mitogenic and cytolytic responses of lymphocytes. A depression (40-60%) of the uptake of 3 H-thymidine by splenic and blood lymphocytes stimulated with either phytohemagglutinin (PHA) or lipopolysaccharide (LPS) was observed during the first 48 hours after transfer of the animals to individual cages. Within 4 days the mitogenic response increased and was comparable to that of animals which had been continuously group-housed. The response continued to increase and by 10 days was enhanced by 2 to 4 fold and remained elevated for at least 8 weeks. Similar changes in activity were observed with both splenic and blood lymphocytes, however, thymic lymphocytes taken from isolated animals demonstrated no change in reactivity to PHA. As with mitogenic responses, the cytolytic activity of splenic lymphocytes was also depressed during the initial days of isolation and as isolation continued, the activity returned to normal and was significantly enhanced (80%) within 5 weeks. These data show that changes in lymphocyte activity are dependent on the duration of exposure to isolated housing conditions and may be a part of the acute, adaptive and chronic phases of the response of rats to the stress of isolation

  7. Antifungal Activity of Bee Venom and Sweet Bee Venom against Clinically Isolated Candida albicans

    Directory of Open Access Journals (Sweden)

    Seung-Bae Lee

    2016-03-01

    Full Text Available Objectives: The purpose of this study was to investigate the antifungal effect of bee venom (BV and sweet bee venom (SBV against Candida albicans (C. albicans clinical isolates. Methods: In this study, BV and SBV were examined for antifungal activities against the Korean Collection for Type Cultures (KCTC strain and 10 clinical isolates of C. albicans. The disk diffusion method was used to measure the antifungal activity and minimum inhibitory concentration (MIC assays were performed by using a broth microdilution method. Also, a killing curve assay was conducted to investigate the kinetics of the anti- fungal action. Results: BV and SBV showed antifungal activity against 10 clinical isolates of C. albicans that were cultured from blood and the vagina by using disk diffusion method. The MIC values obtained for clinical isolates by using the broth microdilution method varied from 62.5 μg/ mL to 125 μg/mL for BV and from 15.63 μg/mL to 62.5 μg/mL for SBV. In the killing-curve assay, SBV behaved as amphotericin B, which was used as positive control, did. The antifungal efficacy of SBV was much higher than that of BV. Conclusion: BV and SBV showed antifungal activity against C. albicans clinical strains that were isolated from blood and the vagina. Especially, SBV might be a candidate for a new antifungal agent against C. albicans clinical isolates.

  8. Spent fuel storage rack for BWR fuel assemblies

    International Nuclear Information System (INIS)

    Machado, O.; Henry, C.W.; Congleton, R.L.; Flynn, W.M.

    1990-01-01

    This patent describes for the use in storing nuclear fuel assemblies in a storage pool containing a coolant and having a pool floor, a fuel rack module. It comprises: a base plate to be disposed generally horizontally on the floor and having a horizontal surface area sufficient to support a fuel assemblies; uniformly spaced openings in the base plate, disposed in rows and columns throughout the surface area; fabricated cells of rectangular cross section extending over alternate openings along each row of the openings, the fabricated cells of each row being uniformly staggered by one opening with respect to the cells of its just adjacent rows so that the fabricated cells form a checkerboard like array; each of the fabricated cells having elongated walls mounted generally vertically on the base plate; each of the corners formed by the walls of each fabricated cell, which corners are internal of the periphery of the array, being disposed as closely adjacent as practicable to and face-to-face with a corner of an adjacent fabricated cell and joined by weld means so that substantially no space exists between adjacent cells. The cells being welded to their bottom ends to the base plate so that a strong compact modular structure is produced; neutron-absorbing means on the external surface of the fabricated cell walls except on the coextensive sections of the outer wall around the periphery of the array; and leveling pads are mounted under the base plate near the periphery thereof and adjustably engage the pool floor and intermediate leveling pads are mounted under cells within the fuel-rack module, the intermediate pads being uniformly disposed

  9. Dynamic response of piping system on rack structure with gaps and frictions

    International Nuclear Information System (INIS)

    Kobayashi, Hiroe; Yoshida, Misutoyo; Ochi, Yoshio

    1989-01-01

    In the seismic design of a piping system on a rack structure, the interaction between the piping system and the rack structure must be evaluated under the condition that the rack structure is not stiff and heavy enough compared with the piping system. Moreover, there are local nonlinearities due to the gap and friction between the piping system and the rack structure. This paper presents the influence of the interaction and the local nonlinearities upon the seismic response by numerical study and a vibration test using a shaking table. In the numerical study, the piping system and the rack structure were represented by the three degrees of freedom mass-spring model taking a vibration mode of the piping system into account. The nonlinearities due to gap and friction were defined as a function of motion and treated as the pseudo force vector (additional applied force) in an equation of motion. From the results of the numerical study and the vibration test, it was clarified that seismic response of both the rack structure and the piping system is reduced by gap and friction. Moreover, the piping system and rack structure can be represented by the three degrees of freedom mass spring model. And the local nonlinearities can be treated by the pseudo force in an equation of motion. (orig.)

  10. Chin Shan analyses show advantages of whole pool multi-rack approach

    International Nuclear Information System (INIS)

    Singh, K.P.; Soler, A.I.

    1991-01-01

    Nuclear fuel storage racks are essentially thin-walled, cellular structures of prismatic cross-section. Although the details of design vary from one supplier to another, certain key physical attributes are common to all designs. For example, all racks feature square cells of sufficient opening size and height to enable insertion and withdrawal of the fuel assembly. The array of cells is positioned in a vertical orientation and is supported off the pool slab surface by four or more support legs. The spent fuel pool is filled with the individual fuel racks. The plenum created by the support legs is essential for proper cooling of the fuel assemblies stored in the rack, which relies on natural convective cooling to extract the heat emitted by the spent fuel. However, it has the insalutary effect of making it kinematically less stable. Regulatory authorities require careful and comprehensive analysis of the response of the racks under the seismic motions postulated for the pool slab. Results from whole pool multi-rack (WPMR) analyses at the Chin Shan and Oyster Creek nuclear plants point up the potential inadequacies of single rack 3D analyses, and show just how important it is to carry out WPMR simulations, despite their abstruseness and high cost. (author)

  11. Borrelidin B: isolation, biological activity, and implications for nitrile biosynthesis.

    Science.gov (United States)

    Schulze, Christopher J; Bray, Walter M; Loganzo, Frank; Lam, My-Hanh; Szal, Teresa; Villalobos, Anabella; Koehn, Frank E; Linington, Roger G

    2014-11-26

    Borrelidin (1) is a nitrile-containing bacterially derived polyketide that is a potent inhibitor of bacterial and eukaryotic threonyl-tRNA synthetases. We now report the discovery of borrelidin B (2), a tetrahydro-borrelidin derivative containing an aminomethyl group in place of the nitrile functionality in borrelidin. The discovery of this new metabolite has implications for both the biosynthesis of the nitrile group and the bioactivity of the borrelidin compound class. Screening in the SToPS assay for tRNA synthetase inhibition revealed that the nitrile moiety is essential for activity, while profiling using our in-house image-based cytological profiling assay demonstrated that 2 retains biological activity by causing a mitotic stall, even in the absence of the nitrile motif.

  12. Combined Cycle Engine Large-Scale Inlet for Mode Transition Experiments: System Identification Rack Hardware Design

    Science.gov (United States)

    Thomas, Randy; Stueber, Thomas J.

    2013-01-01

    The System Identification (SysID) Rack is a real-time hardware-in-the-loop data acquisition (DAQ) and control instrument rack that was designed and built to support inlet testing in the NASA Glenn Research Center 10- by 10-Foot Supersonic Wind Tunnel. This instrument rack is used to support experiments on the Combined-Cycle Engine Large-Scale Inlet for Mode Transition Experiment (CCE? LIMX). The CCE?LIMX is a testbed for an integrated dual flow-path inlet configuration with the two flow paths in an over-and-under arrangement such that the high-speed flow path is located below the lowspeed flow path. The CCE?LIMX includes multiple actuators that are designed to redirect airflow from one flow path to the other; this action is referred to as "inlet mode transition." Multiple phases of experiments have been planned to support research that investigates inlet mode transition: inlet characterization (Phase-1) and system identification (Phase-2). The SysID Rack hardware design met the following requirements to support Phase-1 and Phase-2 experiments: safely and effectively move multiple actuators individually or synchronously; sample and save effector control and position sensor feedback signals; automate control of actuator positioning based on a mode transition schedule; sample and save pressure sensor signals; and perform DAQ and control processes operating at 2.5 KHz. This document describes the hardware components used to build the SysID Rack including their function, specifications, and system interface. Furthermore, provided in this document are a SysID Rack effectors signal list (signal flow); system identification experiment setup; illustrations indicating a typical SysID Rack experiment; and a SysID Rack performance overview for Phase-1 and Phase-2 experiments. The SysID Rack described in this document was a useful tool to meet the project objectives.

  13. Endophytic Fungi Isolated from Coleus amboinicus Lour Exhibited Antimicrobial Activity

    OpenAIRE

    Puji Astuti; Sudarsono Sudarsono; Khoirun Nisak; Giri Wisnu Nugroho

    2014-01-01

    Purpose: Coleus amboinicus is a medicinal plant traditionally used to treat various diseases such as throat infection, cough and fever, diarrhea, nasal congestion and digestive problems. The plant was explored for endophytic fungi producing antimicrobial agents. Methods: Screening for endophytic fungi producing antimicrobial agents was conducted using agar plug method and antimicrobial activity of promising ethyl acetate extracts was determined by disc diffusion assay. Thin layer chromatograp...

  14. Adaptive active vibration isolation – A control perspective

    Directory of Open Access Journals (Sweden)

    Landau Ioan Doré

    2015-01-01

    The paper will review a number of recent developments for adaptive feedback compensation of multiple unknown and time-varying narrow band disturbances and for adaptive feedforward compensation of broad band disturbances in the presence of the inherent internal positive feedback caused by the coupling between the compensator system and the measurement of the image of the disturbance. Some experimental results obtained on a relevant active vibration control system will illustrate the performance of the various algorithms presented.

  15. Isolation and identification of some Bacillus thuringiensis strains with insecticidal activity against Ceratitis capitata

    International Nuclear Information System (INIS)

    Majdoub, Nihed

    2010-01-01

    The aims of the present work is to study the effect of toxins (delta-endotoxins), extracted from different strains of Bacillus thuringiensis on Ceratitis capitata, a devastating of citrus and fruit trees. Strains of B. thuringiensis were isolated from the mud of Sebket Sejoumi. Among 70 isolates tested, 15 showed a significant identicalness activity in which 5 isolates led to mortality rates ≥ 90 pour cent . These mortality rates are caused by endotoxins of B. thuringiensis. Analysis of proteins profiles of different isolates of B. thuringiensis revealed variability between them. The preliminary results of this study encourage us towards the characterization of the insecticidal activity produced by B. thuringiensis strains for large scale application.

  16. Endophytic Fungi Isolated from Coleus amboinicus Lour Exhibited Antimicrobial Activity.

    Science.gov (United States)

    Astuti, Puji; Sudarsono, Sudarsono; Nisak, Khoirun; Nugroho, Giri Wisnu

    2014-12-01

    Coleus amboinicus is a medicinal plant traditionally used to treat various diseases such as throat infection, cough and fever, diarrhea, nasal congestion and digestive problems. The plant was explored for endophytic fungi producing antimicrobial agents. Screening for endophytic fungi producing antimicrobial agents was conducted using agar plug method and antimicrobial activity of promising ethyl acetate extracts was determined by disc diffusion assay. Thin layer chromatography (TLC) - bioautography was performed to localize the bioactive components within the extract. TLC visualization detection reagents were used to preliminary analyze phytochemical groups of the bioactive compounds. Three endophytic fungi were obtained, two of them showed promising potential. Agar diffusion method showed that endophytic fungi CAL-2 exhibited antimicrobial activity against P. aeruginosa, B. subtilis, S. aureus and S. thypi, whilst CAS-1 inhibited the growth of B. subtilis. TLC bioautography of ethyl acetate extract of CAL-2 revealed at least three bands exhibited antimicrobial activity and at least two bands showed inhibition of B. subtilis growth. Preliminary analysis of the crude extracts suggests that bioactive compounds within CAL-2 extract are terpenoids, phenolics and phenyl propanoid compounds whilst the antimicrobial agents within CAS-1 extract are terpenoids, propylpropanoids, alkaloids or heterocyclic nitrogen compounds. These data suggest the potential of endophytic fungi of C. amboinicus as source for antimicrobial agents.

  17. Endophytic Fungi Isolated from Coleus amboinicus Lour Exhibited Antimicrobial Activity

    Directory of Open Access Journals (Sweden)

    Puji Astuti

    2014-12-01

    Full Text Available Purpose: Coleus amboinicus is a medicinal plant traditionally used to treat various diseases such as throat infection, cough and fever, diarrhea, nasal congestion and digestive problems. The plant was explored for endophytic fungi producing antimicrobial agents. Methods: Screening for endophytic fungi producing antimicrobial agents was conducted using agar plug method and antimicrobial activity of promising ethyl acetate extracts was determined by disc diffusion assay. Thin layer chromatography (TLC - bioautography was performed to localize the bioactive components within the extract. TLC visualization detection reagents were used to preliminary analyze phytochemical groups of the bioactive compounds. Results: Three endophytic fungi were obtained, two of them showed promising potential. Agar diffusion method showed that endophytic fungi CAL-2 exhibited antimicrobial activity against P. aeruginosa, B. subtilis, S. aureus and S. thypi, whilst CAS-1 inhibited the growth of B. subtilis. TLC bioautography of ethyl acetate extract of CAL-2 revealed at least three bands exhibited antimicrobial activity and at least two bands showed inhibition of B. subtilis growth. Preliminary analysis of the crude extracts suggests that bioactive compounds within CAL-2 extract are terpenoids, phenolics and phenyl propanoid compounds whilst the antimicrobial agents within CAS-1 extract are terpenoids, propylpropanoids, alkaloids or heterocyclic nitrogen compounds. Conclusion: These data suggest the potential of endophytic fungi of C. amboinicus as source for antimicrobial agents.

  18. Lung Cancer Chemopreventive Activity of Patulin Isolated from Penicillium vulpinum

    Directory of Open Access Journals (Sweden)

    Aymeric Monteillier

    2018-03-01

    Full Text Available Lung cancer is the most lethal form of cancer in the world. Its development often involves an overactivation of the nuclear factor kappa B (NF-κB pathway, leading to increased cell proliferation, survival, mobility, and a decrease in apoptosis. Therefore, NF-κB inhibitors are actively sought after for both cancer chemoprevention and therapy, and fungi represent an interesting unexplored reservoir for such molecules. The aim of the present work was to find naturally occurring lung cancer chemopreventive compounds by investigating the metabolites of Penicillium vulpinum, a fungus that grows naturally on dung. Penicillium vulpinum was cultivated in Potato Dextrose Broth and extracted with ethyl acetate. Bioassay-guided fractionation of this extract was performed by measuring NF-κB activity using a HEK293 cell line transfected with an NF-κB-driven luciferase reporter gene. The mycotoxin patulin was identified as a nanomolar inhibitor of TNF-α-induced NF-κB activity. Immunocytochemistry and Western blot analyses revealed that its mechanism of action involved an inhibition of p65 nuclear translocation and was independent from the NF-κB inhibitor α (IκBα degradation process. Enhancing its interest in lung cancer chemoprevention, patulin also exhibited antiproliferative, proapoptotic, and antimigration effects on human lung adenocarcinoma cells through inhibition of the Wnt pathway.

  19. Evidence for estrogen receptor beta-selective activity of Vitex agnus-castus and isolated flavones.

    Science.gov (United States)

    Jarry, Hubertus; Spengler, Barbara; Porzel, Andrea; Schmidt, Juergen; Wuttke, Wolfgang; Christoffel, Volker

    2003-10-01

    Recent cell culture experiments indicated that extracts of Vitex agnus-castus (VAC) may contain yet unidentified phytoestrogens. Estrogenic actions are mediated via estrogen receptors (ER). To investigate whether VAC compounds bind to the currently known isoforms ERalpha or ERss, ligand binding assays (LBA) were performed. Subtype specific ER-LBA revealed a binding of VAC to ERss only. To isolate the ERss-selective compounds, the extract was fractionated by bio-guidance. The flavonoid apigenin was isolated and identified as the most active ERss-selective phytoestrogen in VAC. Other isolated compounds were vitexin and penduletin. These data demonstrate that the phytoestrogens in VAC are ERss-selective.

  20. Enzyme activity screening of thermophilic bacteria isolated from Dusun Tua Hot Spring, Malaysia

    Science.gov (United States)

    Msarah, Marwan; Ibrahim, Izyanti; Aqma, Wan Syaidatul

    2018-04-01

    Thermophilic bacteria have biotechnological importance due to the availability of unique enzymes which are stable in extreme circumstances. The aim of this study includes to isolate thermophilic bacteria from hot spring and screen for important enzyme activities. Water samples from the Dusun Tua Hot Spring were collected and the physiochemical characterisation of water was measured. Eight thermophilic bacteria were isolated and determined to have at least three strong enzyme activity including protease, lipase, amylase, cellulase, pectinase and xylanase. The results showed that HuluC2 displayed all the enzyme activities and can be further studied.

  1. A Study on Criticality Safety Parameters of New Fuel Storage Rack Design

    Energy Technology Data Exchange (ETDEWEB)

    Ahn, Joon Gi; Kim, Hyeong Heon [KEPCO E and C, Daejeon (Korea, Republic of)

    2012-05-15

    The dry new fuel storage rack shall maintain the subcritical condition (i.e., k-eff < 0.95) when fully flooded with water and the k-eff will not exceed 0.98 even assuming that the optimum moderation causes the highest reactivity. Thus, the design parameters of the new fuel rack are determined optimally by considering both the full density water flooding condition and the optimum moderation condition. The behavior of the keff as the variation of design parameter of the new fuel rack was investigated as the function of the moderating water density

  2. Criticality and Its Uncertainty Analysis of Spent Fuel Storage Rack for Research Reactor

    International Nuclear Information System (INIS)

    Han, Tae Young; Park, Chang Je; Lee, Byung Chul

    2011-01-01

    For evaluating the criticality safety of spent fuel storage rack in an open pool type research reactor, a permissible upper limit of criticality should be determined. It can be estimated from the criticality upper limit presented by the regulatory guide and an uncertainty of criticality calculation. In this paper, criticalities for spent fuel storage rack are carried out at various conditions. The calculation uncertainty of MCNP system is evaluated from the calculation results for the benchmark experiments. Then, the upper limit of criticality is determined from the uncertainties and the calculated criticality of the spent fuel storage rack is evaluated

  3. A Study on Criticality Safety Parameters of New Fuel Storage Rack Design

    International Nuclear Information System (INIS)

    Ahn, Joon Gi; Kim, Hyeong Heon

    2012-01-01

    The dry new fuel storage rack shall maintain the subcritical condition (i.e., k-eff < 0.95) when fully flooded with water and the k-eff will not exceed 0.98 even assuming that the optimum moderation causes the highest reactivity. Thus, the design parameters of the new fuel rack are determined optimally by considering both the full density water flooding condition and the optimum moderation condition. The behavior of the keff as the variation of design parameter of the new fuel rack was investigated as the function of the moderating water density

  4. Antioxidant Activity of Chemical Constituents Isolated from Pithecellobium clypearia

    Directory of Open Access Journals (Sweden)

    Lingzhi Li

    2015-04-01

    Full Text Available Phytochemical investigation of the aqueous extract of Pithecellobium clypearia afforded one n ovel compound, clypearoside A(1, and one new stereoisomer , (--(2S,3S-epigallocatechin-7-gallate(2 and four known ones (3-6. Their structures were elucidated on the basis of spectroscopic data, especially 2D NMR, HRESIMS and ECD spectra. Compounds 2-4 showed strong activity against DPPH (diphenylpicrylhydrazyl and ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonate with an IC 50 value of 11.9-13.2 μg/mL (DPPH and 4.5-6.4 μg/mL (ABTS.

  5. Isolation, identification and antagonistic activity evaluation of actinomycetes in barks of nine trees

    Directory of Open Access Journals (Sweden)

    Wang Dong-sheng

    2017-01-01

    Full Text Available Actinomycetes are important producers of novel bioactive compounds. New sources need to be explored for isolating previously unknown bioactive compound-producing actinomycetes. Here we evaluated the potential of bark as a natural source of novel bioactive actinomycete species. Bark samples were collected from nine tree species at different elevations (1600-3400 ma.s.l. on Qin Mountain, Shaanxi Province, China. Actinomycetes were cultivated, enumerated and isolated using serial dilution and spread-plate techniques. The antimicrobial activity of actinomycete isolates was analyzed using an agar block method against 15 typical bacterial and fungal species and plant pathogens. The dominant isolates were identified by 16S rRNA-based sequence analysis. Results showed that actinomycete counts in bark samples of Quercus liaotungensis Koidz. was the highest among all trees species tested. The numbers of actinomycete species in bark samples were highest in Q. aliena var. acutiserrata and Spiraea alpina Pall. Antagonistic activity wasdetected in approximately 54% of the actinomycete isolates. Of these, 20 isolates (25% showed broad-spectrum antagonistic activity against ≥5 of the microorganisms tested. In conclusion, the bark on coniferous and broadleaf trees possesses a high diversity of actinomycetes and serves as a natural source of bioactive compound-producing actinomycetes.

  6. Peroxidase Activity in Poplar Inoculated with Compatible and Incompetent Isolates of Paxillus involutus

    Directory of Open Access Journals (Sweden)

    ABDUL GAFUR

    2007-06-01

    Full Text Available Peroxidase activity of the hybrid poplar Populus×canescens (Ait. Sm. (= P. tremula L. × P. alba L. inoculated with compatible and incompetent isolates of Paxillus involutus (Batsch Fr. was investigated. Screening of the ectomycorrhizal fungal isolates was initiated with exploration of mycelial growth characteristics and mycorrhizal ability in vitro with poplar. Both traits varied within the fungus although they did not seem to be genetically correlated. While isolates SCO1, NAU, and 031 grew faster than others, only isolates MAJ, SCO1, and 031 were able to form ectomycorrhiza with poplar. Isolates MAJ (compatible and NAU (incompetent were subsequently selected for further experiments. Activity of peroxidase, one of the defense-related enzymes, was examined in pure culture and short root components of compatible and incompetent interactions between poplar and P. involutus. Peroxidase activities increased significantly in poplar inoculated with incompetent isolate of the fungus compared to control, while induction of the same enzyme was not detected in compatible associations.

  7. Investigation of the Antimicrobial Activity of Bacillus licheniformis Strains Isolated from Retail Powdered Infant Milk Formulae.

    Science.gov (United States)

    Alvarez-Ordóñez, Avelino; Begley, Máire; Clifford, Tanya; Deasy, Thérèse; Considine, Kiera; O'Connor, Paula; Ross, R Paul; Hill, Colin

    2014-03-01

    This study investigated the potential antimicrobial activity of ten Bacillus licheniformis strains isolated from retail infant milk formulae against a range of indicator (Lactococcus lactis, Lactobacillus bulgaricus and Listeria innocua) and clinically relevant (Listeria monocytogenes, Staphylococcus aureus, Streptococcus agalactiae, Salmonella Typhimurium and Escherichia coli) microorganisms. Deferred antagonism assays confirmed that all B. licheniformis isolates show antimicrobial activity against the Gram-positive target organisms. PCR and matrix-assisted laser desorption ionization time-of-flight mass spectrometry analyses indicated that four of the B. licheniformis isolates produce the bacteriocin lichenicidin. The remaining six isolates demonstrated a higher antimicrobial potency than lichenicidin-producing strains. Further analyses identified a peptide of ~1,422 Da as the most likely bioactive responsible for the antibacterial activity of these six isolates. N-terminal sequencing of the ~1,422 Da peptide from one strain identified it as ILPEITXIFHD. This peptide shows a high homology to the non-ribosomal peptides bacitracin and subpeptin, known to be produced by Bacillus spp. Subsequent PCR analyses demonstrated that the six B. licheniformis isolates may harbor the genetic machinery needed for the synthesis of a non-ribosomal peptide synthetase similar to those involved in production of subpeptin and bacitracin, which suggests that the ~1,422 Da peptide might be a variant of subpeptin and bacitracin.

  8. [Isolation of endophytic fungi from medicinal plant Brucea javanica and their microbial inhibition activity].

    Science.gov (United States)

    Liang, Zi-Ning; Zhu, Hua; Lai, Kai-Ping; Chen, Long

    2014-04-01

    To isolate and identify endophytic fungi from Brucea javanica, and to detect the antimicrobial activity of these strains. Endophytic fungi were isolated by tissue inoculation culture and identified by conventional morphological characteristic method. Seven kinds of pathogenic fungi and three kinds of bacteria were used as targeting microbes to test microbial inhibition activities by agar plate antagonistic action and modified agar gel diffusion methods, respectively. A total of 83 endophytic fungi strains were isolated from the root, stem, leaf and fruit of Brucea javanica. 34 strains were obtained from the stem, 32 strains were obtained from the leaf, 15 strains were isolated from the root and 2 strains came from the fruit. These 73 strains which had been identified attribute to 5 orders, 6 families and 12 genera. For the isolated strains, 14 strains had antifungal activities against at least one pathogenic fungi, 9 strains showed antibacterial activities against one or more bacteria. Especially, the strain YJ-17 which belonged to Phomopsis genus showed the best inhibitory effect on the targeting microbes. The endophytic fungi from Brucea javanica show diversity and microbial inhibition activity, and are worthy for further study on plant disease controlling.

  9. FACTORS AFFECTED DECARBOXYLATION ACTIVITY OF ENTEROCOCCUS FAECIUM ISOLATED FROM RABBIT

    Directory of Open Access Journals (Sweden)

    František Buňka

    2012-04-01

    Full Text Available Normal 0 21 false false false SK JA X-NONE Biogenic amines (BA are basic nitrogenous compounds formed mainly by decarboxylation of amino acids. There are generated in course of microbial, vegetable and animal metabolisms. The aim of the study was to monitor factors affected production of biogenic amines by Enterococcus faecium, which is found in rabbit meat. Biogenic amines were analyzed by means of UPLC (ultrahigh performance liquid chromatography equipped with a UV/VIS DAD detector. Decarboxylation activity of E. faecium was mainly influenced by the cultivation temperature and the amount of NaCl in this study. E. faecium produced most of the monitored biogenic amines levels: tyramine ˂2500 mg.l-1; putrescine ˂30 mg.l-1; spermidine ˂10 mg.l-1 and cadaverine ˂5 mg.l-1.doi:10.5219/182

  10. Exoenzyme activity and possibility identification of Candida dubliniensis among Candida albicans species isolated from vaginal candidiasis.

    Science.gov (United States)

    Jafari, Maryam; Salari, Samira; Pakshir, Keyvan; Zomorodian, Kamiar

    2017-09-01

    Vulvovaginal candidiasis (VVC) or vaginal candidiasis is a common fungal infection of the genitals causing inflammation, irritation, itching, and vaginal discharge. Common yeast infections are caused by the yeast species C. albicans. However, there are other species of Candida such as C. dubliniensis which are considered as the causative agents of this infection. Hydrolytic enzymes such as proteinase and coagulase are known as virulence factors. The aim of this study was the molecular confirmation and differentiation of C. dubliniensis among C. albicans strains isolated from women with vulvovaginal candidiasis by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) and the evaluation of proteinase and coagulase activities. A total of 100 C. albicans strains isolated from women with vulvovaginal candidiasis referred to Shiraz medical clinics were enrolled in the study. All the isolates were primarily identified by conventional methods. PCR-RFLP method was used for the confirmation and identification of C. albicans and C. dubliniensis. Moreover, in vitro proteinase and coagulase activities of these isolates were evaluated using bovine serum albumin media and classical rabbit plasma tube test. As a result, PCR-RFLP identified 100% of the isolates as C. albicans, and no C. dubliniensis could be identified in this study. 84% of the isolates showed proteinase activity, whereas coagulase activity was only detected in 5% of the isolates. This study reveals that C. dubliniensis plays no role in vaginal candidiasis in Iranian patients. Proteinase production could be an essential virulence factor in C. albicans pathogenicity, but coagulase activity has less potential in this matter. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Materials Science Research Rack Onboard the International Space Station Hardware and Operations

    Science.gov (United States)

    Lehman, John R.; Frazier, Natalie C.; Johnson, Jimmie

    2012-01-01

    The Materials Science Research Rack (MSRR) is a research facility developed under a cooperative research agreement between NASA and ESA for materials science investigations on the International Space Station (ISS). MSRR was launched on STS-128 in August 2009, and is currently installed in the U.S. Destiny Laboratory Module. Since that time, MSRR has performed virtually flawlessly, logging more than 620 hours of operating time. The MSRR accommodates advanced investigations in the microgravity environment on the ISS for basic materials science research in areas such as solidification of metals and alloys. The purpose is to advance the scientific understanding of materials processing as affected by microgravity and to gain insight into the physical behavior of materials processing. MSRR allows for the study of a variety of materials including metals, ceramics, semiconductor crystals, and glasses. Materials science research benefits from the microgravity environment of space, where the researcher can better isolate chemical and thermal properties of materials from the effects of gravity. With this knowledge, reliable predictions can be made about the conditions required on Earth to achieve improved materials. MSRR is a highly automated facility with a modular design capable of supporting multiple types of investigations. Currently the NASA-provided Rack Support Subsystem provides services (power, thermal control, vacuum access, and command and data handling) to the ESA developed Materials Science Laboratory (MSL) which accommodates interchangeable Furnace Inserts (FI). Two ESA-developed FIs are presently available on the ISS: the Low Gradient Furnace (LGF) and the Solidification and Quenching Furnace (SQF). Sample-Cartridge Assemblies (SCAs), each containing one or more material samples, are installed in the FI by the crew and can be processed at temperatures up to 1400 C. Once an SCA is installed, the experiment can be run by automatic command or science conducted via

  12. Antimicrobial and acetylcholinesterase inhibitory activities of Buddleja salviifolia (L.) Lam. leaf extracts and isolated compounds.

    Science.gov (United States)

    Pendota, S C; Aderogba, M A; Ndhlala, A R; Van Staden, J

    2013-07-09

    Buddleja salviifolia leaves are used for the treatment of eye infections and neurodegenerative conditions by various tribes in South Africa. This study was designed to isolate the phenolic constituents from the leaf extracts of Buddleja salviifolia and evaluate their antimicrobial and acetylcholinesterase (AChE) activities. Three phenolic compounds were isolated from the ethyl acetate fraction of a 20% aqueous methanol leaf extract of Buddleja salviifolia using Sephadex LH-20 and silica gel columns. Structure elucidation of the isolated compounds was carried out using spectroscopic techniques: mass spectrometry (ESI-TOF-MS) and NMR (1D and 2D). The extracts and isolated compounds were evaluated for antimicrobial and acetylcholinesterase activities using the microdilution technique. The bacteria used for the antimicrobial assays were Gram-positive Bacillus subtilis and Staphylococcus aureus and Gram-negative Escherichia coli and Klebsiella pneumoniae. The isolated compounds were characterized as: 4'-hydroxyphenyl ethyl vanillate (1) a new natural product, acteoside (2) and quercetin (3). The crude extract, fractions and the isolated compounds from the leaves of the plant exhibited a broad spectrum of antibacterial activity. The EtOAc fraction exhibited good activity against Bacillus subtilis and Staphylococcus aureus with MIC values ranging from 780.0 to 390.0 µg/mL. Isolated compound 2 exhibited good activity against Staphylococcus aureus with an MIC value of 62.5 µg/mL. The hexane and DCM fractions of leaves showed the best activity against Candida albicans with MIC and MFC values of 390.0 µg/mL. In the AChE inhibitory test, among the tested extracts, the hexane fraction was the most potent with an IC50 value of 107.4 µg/mL, whereas for the isolated compounds, it was compound (3) (quercetin) with an IC50 value of 66.8 µg/mL. Activities demonstrated by the extracts and isolated compounds support the ethnopharmacological use of Buddleja salviifolia against eye

  13. In vitro antimicrobial activity of extracts and isolated compound from Dalbergia stipulacea Roxb. leaves

    Science.gov (United States)

    Kumar, Arvind; Bhat, Tahir Ahmad; Singh, Rattan Deep

    2017-07-01

    The study was designed to examine the in vitro antimicrobial efficacy of extracts and isolated compound of Dalbergia stipulacea. Combined extracts (chloroform and methanol) of plant leaves fractionated with n-butanol loaded with column afforded a flavonoid glycoside compound identified as luteolin 4'-rutinoside. Different extracts and isolated compound exhibited pronounced antibacterial and antifungal varied activities against four bacteria (Clostridium acetobutylinium, Bacillus subtilis, Streptococcus mutans, and Pseudomonas sp.) and one fungus (Candida albicans) susceptibility were determined using disc diffusion method. The minimum inhibitory concentration (MIC) of extracts and isolated compounds was determined by broth dilution method. The maximum activity was shown by chloroform extract against C. albicans with a zone of inhibition of 17 mm and minimum activity was displayed by methanolic extract against Pseudomonas sp. with 5 mm. However, isolated compound has shown maximum activity against Pseudomonas sp. with 15 mm. The MIC values higher in methanol extract against Pseudomonas sp. and isolated compound shows good against Pseudomonas sp. and B. subtilis. Our findings indicate that plant could be used as a good antimicrobial agent in food, pharmaceutical and bio-pesticide industries.

  14. In vitro activities of antifungal drugs against environmental Exophiala isolates and review of the literature.

    Science.gov (United States)

    Gülmez, Dolunay; Doğan, Özlem; Boral, Barış; Döğen, Aylin; İlkit, Macit; de Hoog, G Sybren; Arikan-Akdagli, Sevtap

    2018-04-03

    Exophiala is a genus of black fungi isolated worldwide from environmental and clinical specimens. Data on antifungal susceptibility of Exophiala isolates are limited and the methodology on susceptibility testing is not yet standardized. In this study, we investigated in vitro antifungal susceptibilities of environmental Exophiala isolates. A total of 87 Exophiala isolated from dishwashers or railway ties were included. CLSI M38-A2 microdilution method with modifications was used to determine antifungal susceptibility for fluconazole, voriconazole, posaconazole, itraconazole, amphotericin B, and terbinafine. Minimum inhibitory concentration (MIC) values were determined visually at 48h, 72h, and 96h. MIC-0 endpoint (complete inhibition of growth) was used for amphotericin B and azoles, except fluconazole, for which MIC-2 endpoint (~50% inhibition compared to growth control) was used. Both MIC-0 and MIC-1 (~80% inhibition compared to growth control) results were analysed for terbinafine, to enable comparison with previous studies. Fungal growth was sufficient for determination of MICs at 48h for all isolates except two Exophiala dermatitidis strains. At 72h, most active antifungal agents according to GM MIC were voriconazole and terbinafine, followed by posaconazole, itraconazole, and amphotericin B in rank order of decreasing activity. While amphotericin B displayed adequate in vitro activity despite relatively high MICs, fluconazole showed no meaningful antifungal activity against Exophiala. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  15. Antibacterial Activity of Rhodomyrtus tomentosa (Aiton Hassk. Leaf Extract against Clinical Isolates of Streptococcus pyogenes

    Directory of Open Access Journals (Sweden)

    Surasak Limsuwan

    2012-01-01

    Full Text Available Ethanol extract of Rhodomyrtus tomentosa (Aiton Hassk. leaf was evaluated for antibacterial activity against 47 clinical isolates of Streptococcus pyogenes. The extract exhibited good anti-S. pyogenes activity against all the tested isolates with similar minimum inhibitory concentration (MIC, 3.91–62.5 μg mL−1 and minimum bactericidal concentration (MBC, 3.91–62.5 μg mL−1 ranges. No surviving cells were detected at 16 h after treatment with 8 × MIC of the extract. The extract-treated cells demonstrated no lysis and cytoplasmic leakage through the bacterial membrane. Electron micrographs further revealed that the extract did not cause any dramatic changes on the treated cells. Rhodomyrtone, an isolated compound, exhibited good anti-S. pyogenes activity (14 isolates, expressed very low MIC (0.39–1.56 μg mL−1 and MBC (0.39-1.56 μg mL−1 values. Rhodomyrtus tomentosa leaf extract and rhodomyrtone displayed promising antibacterial activity against clinical isolates of S. pyogenes.

  16. Isolation, Characterization and Activity of the Flowers of Rhododendron arboreum (Ericaceae

    Directory of Open Access Journals (Sweden)

    Pankaj Kumar Sonar

    2012-01-01

    Full Text Available The flowers of Rhododendron arboreum have been reported to possess certain polyphenolic compounds. Thus, this study was aimed at the anti-microbial and phytochemical screening of the flowers. Important bioactive agents like steroids, saponins and flavonoids were detected in the flowers. Quercetin (a flavonoid was isolated from the diethyl ether fraction of alcoholic extract by solvent-solvent extraction method. Isolated quercetin was identified and characterized by chemical tests, M.P., TLC, paper chromatography (with authentic marker and spectroscopic methods like UV-Visible, FT-IR, 1HNMR, 13CNMR and Mass spectroscopy. The anti-microbial activity of the alcoholic and aqueous extract and isolated quercetin were investigated against five bacterial and two fungal strains by agar well-diffusion method. The activity was found to be concentration dependent. Ethanolic extract was found to be more active in comparison to the aqueous extract. Hence, isolation was done with ethanolic extract. The lowest effective concentration of quercetin was found to be 12.5 mg/ml against S. aureus and P. aeruginosa. Both extracts and isolated quercetin were found ineffective against fungal strains. Quercetin may be one of the components responsible for the observed anti-microbial activity of the plant.

  17. ENEA activities on seismic isolation of nuclear and non-nuclear structures

    International Nuclear Information System (INIS)

    Martelli, A.; Masoni, P.; Forni, M.; Indirli, M.; Spadoni, B.; Di Pasquale, G.; Lucarelli, V.; Sano, T.; Bonacina, G.; Castoldi, A.

    1989-01-01

    Work on seismic isolation of nuclear and non-nuclear structures was started by ENEA in cooperation with ISMES in 1988. The first activity consisted of a proposal for guidelines for seismically isolated nuclear plants using high-damping, steel-laminated elastomer bearings. This is being performed in the framework of an agreement with General Electric Company. Furthermore, research and development work has been defined and recently initiated to support development of the seismic isolation guidelines as well as that of qualification procedures for seismic isolation systems in general. The present R and D work includes static and dynamic experiments on single bearings, shake table tests with multi-axial simultaneous excitations on reduced-scale mockups of isolated structures supported by multiple bearings, and dynamic tests on large-scale isolated structures with on-site test techniques. It also includes the development and validation of finite-element nonlinear models of the single bearings, as well as those of simplified design tools for the analysis of the isolated structures dynamic behavior. Extension of this work is foreseen in a wider national frame

  18. Isolation and characterization of Bacillus thuringiensis strains active against Elasmopalpus lignosellus (Zeller, 1848 (Lepidoptera, Pyralidae

    Directory of Open Access Journals (Sweden)

    Janaina Zorzetti

    2017-08-01

    Full Text Available Elasmopalpus lignosellus (Zeller, 1848 (Lepidoptera, Pyralidae is an insect pest of 60 economically important crops, including sugarcane, wheat, soybean, rice, beans, sorghum, peanuts, and cotton. The aim of this work was to select and characterize Bacillus thuringiensis isolates with insecticidal activity against E. Lignosellus that could be used as an alternative method of control. Selective bioassays were done to evaluate the toxicity of 47 isolates against first instar larvae of E. lignosellus. For the most toxic bacterial strains, the lethal concentration (LC50 was estimated and morphological, biochemical and molecular methods were used to characterize the isolates. Among the 47 isolates tested, 12 caused mortality above 85% and showed LC50 values from 0.038E+8 to 0.855E+8 spores mL-1. Isolates BR83, BR145, BR09, BR78, S1534, and S1302 had the lowest LC50 values and did not differ from the standard HD-1 strain; the exception was BR83.The protein profiles produced bands with molecular masses of 60-130 kDa. The genes cry1, cry2, cry3, and cry11 were identified in the molecular characterization. The morphological analysis identified three different crystal inclusions: bipyramidal, spherical and cuboidal. Among the tested isolates, 12 isolates have potential for biotechnological control of E. Lignosellus by development of new biopesticides or genetically modified plants.

  19. Phospholipase and proteinase activities of Candida spp. isolates from vulvovaginitis in Iran.

    Science.gov (United States)

    Shirkhani, S; Sepahvand, A; Mirzaee, M; Anbari, K

    2016-09-01

    This study aims to characterize phospholipase and proteinase activities of Candida isolates from 82 vulvovaginal candidiasis (VVC) and to study the relationship of these activities with vulvovaginitis. Totally 82 Candida isolates from vagina samples of VVC patients were randomly collected over the period between September and December 2014 from hospitalized patients at the general hospitals of Lorestan province, Iran. Isolates were previously identified by conventional mycological methods. The phospholipase and proteinase activities were evaluated by Egg yolk agar, Tween 80 opacity medium and agar plate methods. The most common Candida species was identified Candida albicans (n=34, 41.5%), followed by Candida famata (n=13, 15.8%), Candida tropicalis (n=11, 13.4%), and Candida parapsilosis (n=9, 11%). The most phospholipase activity was observed in Candida colliculosa (40%), followed by C. famata (38.5%), and Candida krusei (33.3%). The findings revealed that the correlation between phospholipase production by Candida spp. and the presence of VVC was not found to be statistically significant (P=0.91). All Candida spp. exhibited considerable proteinase activity; so that 100% of C. colliculosa, C. parapsilosis, Candida kefyr, and Candida intermedia isolates produced high proteinase activity with Pz 4+ scores. There was a significant correlation between proteinase production by Candida spp. and the presence of VVC (P=0.009). The obtained findings revealed that Candida spp. isolates may produce both virulence factors, phospholipase and proteinase. Although the phospholipase production was only observed in <40% of the isolates; however there was a significant association between proteinase production by Candida spp. and VVC. Copyright © 2016. Published by Elsevier Masson SAS.

  20. Isolation of Biosurfactant–Producing Bacteria with Antimicrobial Activity against Bacterial Pathogens

    Directory of Open Access Journals (Sweden)

    Siripun Sarin

    2011-01-01

    Full Text Available The aims of this research were to study biosurfactant producing bacteria isolated from soil and to determine their property and efficiency as biosurfactants in order to inhibit bacterial pathogens. The result showed that there were 8 bacterial isolates out of 136 isolates of the total biosurfactant producing bacteria screened that exhibited the diameter of clear zone more than 1.5 cm. in the oil spreading test. The highest potential of emulsifying activity (%EA24 of 54.4 and the maximum additive concentration, (%MAC of 24.2 was obtained from the fermentation broth of the G7 isolate which the G7 isolate was later identified as Pseudomonas fluorescens. Escherichia coli, Staphylococcus aureus and Psuedomonas aeruginosa were the tested bacterial pathogens that were most sensitive to the acid precipitated biosurfactant obtained from P. fluorescens G7 with the lowest minimum inhibitory concentration (MIC of 41.6 mg/ml and minimum bactericidal concentration (MBC of 41.6 mg/ml compared with the acid precipitated bisurfactants of the other isolates used in the antimicrobial activity test. The type of the separated crude biosurfactant produced by P. fluorescens G7 analyzed later by using the rhamose test, TLC and FT-IR techniques was rhamnolipid.

  1. Isolation, Characterization, and Genetic Diversity of Ice Nucleation Active Bacteria on Various Plants

    Directory of Open Access Journals (Sweden)

    DIANA ELIZABETH WATURANGI

    2009-06-01

    Full Text Available Ice nucleation active (INA bacteria is a group of bacteria with the ability to catalyze the ice formation at temperature above -10 °C and causing frost injury in plants. Since, most of the literature on INA bacteria were from subtropical area, studies of INA bacteria from tropical area are needed. We sampled eight fruits and 36 leaves of 21 plant species, and then identified through biochemical and genetic analysis. INA bacteria were characterized for INA protein classification, pH stability, and optimization of heat endurance. We discovered 15 INA bacteria from seven plants species. Most of bacteria are oxidase and H2S negative, catalase and citrate positive, gram negative, and cocoid formed. These INA bacteria were classified in to three classes based on their freezing temperature. Most of the isolates were active in heat and pH stability assay. Some isolates were analysed for 16S rRNA gene. We observed that isolates from Morinda citrifolia shared 97% similiarity with Pseudomonas sp. Isolate from Piper betle shared 93% similarity with P. pseudoalcaligenes. Isolate from Carica papaya shared 94% similarity with Pseudomonas sp. While isolate from Fragaria vesca shared 90% similarity with Sphingomonas sp.

  2. Isolation, Characterization, and Genetic Diversity of Ice Nucleation Active Bacteria on Various Plants

    Directory of Open Access Journals (Sweden)

    DIANA ELIZABETH WATURANGI

    2009-06-01

    Full Text Available Ice nucleation active (INA bacteria is a group of bacteria with the ability to catalyze the ice formation at temperature above -10 oC and causing frost injury in plants. Since, most of the literature on INA bacteria were from subtropical area, studies of INA bacteria from tropical area are needed. We sampled eight fruits and 36 leaves of 21 plant species, and then identified through biochemical and genetic analysis. INA bacteria were characterized for INA protein classification, pH stability, and optimization of heat endurance. We discovered 15 INA bacteria from seven plants species. Most of bacteria are oxidase and H2S negative, catalase and citrate positive, gram negative, and cocoid formed. These INA bacteria were classified in to three classes based on their freezing temperature. Most of the isolates were active in heat and pH stability assay. Some isolates were analysed for 16S rRNA gene. We observed that isolates from Morinda citrifolia shared 97% similiarity with Pseudomonas sp. Isolate from Piper betle shared 93% similarity with P. pseudoalcaligenes. Isolate from Carica papaya shared 94% similarity with Pseudomonas sp. While isolate from Fragaria vesca shared 90% similarity with Sphingomonas sp.

  3. Antimicrobial activity screening of marine bacteria isolated from Port Klang and Port Tanjung Pelepas

    Science.gov (United States)

    Ibrahim, Nik Nuraznida Nik; Usup, Gires; Ahmad, Asmat

    2018-04-01

    Over the past ten years, marine natural product researchers have expanded the scope of their studies from macroorganisms such as algae to marine microorganisms. The marine environment is believed to be able to provide novel lead against pathogenic microbes that are evolving and developing resistance to existing pharmaceutical agents. In this study, a total of 150 bacterial isolates isolated from Port Klang and Port Tanjung Pelepas were screened for antimicrobial activity against Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Entrococcus, faecalis, Pseudomonas aeruginosa and Methicillin-Resistance Staphylococcus aureus (MRSA). Only 10 isolates: PW01, PW02, PB03, and PS (04, 05, 06, 07, 08, 09, and 10) showed strong antibacterial activity. Based on the strongest activity, isolates PW01 and PW02 were selected for secondary screening using well diffusion assay. The dichloromethane extract of Pseudomonas sp. PW01 showed activity against S. aureus (15±0 mm), V. parahaemolyticus (25±1.63 mm) and MRSA (18±0.81 mm). Meanwhile, the diethyl ether extract of Pseudomonas sp. PW02 showed active activity against S. aureus (10±0 mm), V. parahaemolyticus (30±0.94 mm), MRSA (30±0.94 mm), E. coli (22±1.25 mm) and E. faecalis (26±0 mm). Through this study, it was suggested that marine microorganisms may represent an untapped reservoir of biodiversity capable of synthesizing antimicrobial molecules.

  4. Combat Ration Network for Technology Implementation. Retort Racks for Polymeric Trays in 1400 Style Spray Retorts

    National Research Council Canada - National Science Library

    Bruins, Henderikus B; Coburn, John F

    2003-01-01

    The objective of this project was to design a retort rack that would maximize the retort capacity of a 1400 style spray retort and to select a material that would withstand the harsh retort spray environment...

  5. Arbitrary vehicle steering characteristics with changing ratio rack and pinion transmission

    Directory of Open Access Journals (Sweden)

    András G Bendefy

    2015-12-01

    Full Text Available In order to achieve arbitrary steering characteristics at vehicles, a steering mechanism was developed, in which changing ratio rack and pinion connections have been applied. In contrary to a regular steering mechanism where only a single rack is used, two racks were applied in order to make arbitrary characteristics possible. The turning wheel’s required motion functions had to be defined first, thereafter could we determine the changing ratio rack and pinion geometry which produces this motion. First, a simplified two-dimensional mockup was created in order to study the difficulties and possibilities of a real construction. Later, a fully functional assembly was designed and manufactured to make further experiments possible.

  6. Nonlinear analyses of spent-fuel racks for consolidated fuel loading

    International Nuclear Information System (INIS)

    Kabir, A.F.; Godha, P.C.; Malik, L.E.; Bolourchi, S.

    1987-01-01

    Storage racks for spent-fuel assemblies in nuclear power plants are designed to withstand various combinations of loads generated by gravity, seismic, thermal, and accidental fuel drops. Due to the need for storing increased amounts of spent fuel in the existing fuel pools, many nuclear power utilities are evaluating existing fuel racks to safely carry the additional loads. The current study presents the seismic analyses of existing fuel racks of Northeast Utility Company's Millstone Unit Number 1 (BWR Mark I) nuclear plant to accommodate a 2:1 fuel consolidation. This objective requires rigorous nonlinear analyses to establish the full available capacities of the racks and thereby avoid expensive modifications or minimize any needed upgrades

  7. Antioxidant Activity of Whey from Milk Fermented with Lactobacillus Species Isolated from Nigerian Fermented Foods

    Directory of Open Access Journals (Sweden)

    Ifeoma Korie

    2010-01-01

    Full Text Available Eight Lactobacillus isolates obtained from five indigenous fermented foods (ogi, ogi baba, wara, kunnu and ugba were investigated. Wara is a dairy-based food while the others are not dairy-based. The bacteria were isolated on MRS agar and purified by successive streaking on the same medium. The whey fraction of skimmed milk fermented with each isolate was assayed for radical scavenging effects using 2,2-diphenyl-1-picrylhydrazyl (DPPH radical. All the whey fractions showed radical scavenging activities. The five isolates with the highest activities were selected. On the basis of Gram stain reaction, cellular morphology, biochemical tests and carbohydrate utilization profiles they were identified as strains of Lactobacillus brevis, L. fermentum, L. plantarum, L. casei and L. delbrueckii. The antioxidant activities of whey fractions from 24-hour fermentations with the selected organisms were investigated using both radical scavenging effects and lipid peroxidation inhibitory activity. The radical scavenging activity was generally higher than the lipid peroxidation inhibition, except in the L. plantarum strain, which did not show any significant difference in both activities. The probiotic potential of the isolates was evaluated by pH and bile tolerance. None of the selected isolates showed any growth at pH=2.0 but L. casei and L. delbrueckii survived at this pH. Four of the five selected isolates were able to grow in 0.5 % dehydrated bile, with L. casei strain showing the highest level of growth, followed by L. delbrueckii. L. plantarum strain was not bile tolerant. The ability of L. casei and L. delbrueckii strains to survive at pH=2 and grow in the presence of bile indicates that the isolates may be able to colonize the gastrointestinal tract. The findings of this study indicate that Lactobacillus strains isolated from indigenous Nigerian fermented foods could be useful as starter cultures to provide antioxidants in food and that fermented milk

  8. Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts

    Directory of Open Access Journals (Sweden)

    E. Mikulska

    2015-01-01

    Full Text Available This paper deals with attempts to elaborate a simple method of spinach chloroplast isolation ensuring a high proportion of intact chloroplasts. We obtained 3 preparations of isolated chloroplasts. Several preliminary analyses of the obtained chloroplast fraction were also performed. Phosphorus compounds, total protein and the enzyme activities of RNase, DNase and GPase were determined. We found: 0,36-0,59% of RNA, 0,19-0,24% of DNA, 2,1-2,9% of phospholipids and 26-28% of protein. RNase activity was very high.

  9. Isolation of strains of Bacillus thuringiensis insecticidal biological activity against Ceratitis capitata

    International Nuclear Information System (INIS)

    Hmaied, Ezzedine; Ben Mbarek, Wael

    2010-01-01

    The present work is to study the effect of toxins (δ-endotoxins) extracted from strains of Bacillus thuringiensis isolated from the mud on the fly Sabkhat Dejoumi Ceratitis capitata, a pest of citrus and fruit trees. Among 51 isolated tested, 15 showed a very significant insecticidal activity, characterized by mortality rates exceeding 80 pour cent. These mortality rates are caused by endotoxins of Bt revealed variability between them. The preliminary results of this study encourage us towards the characterization of the insecticidal activity produced by strains of Bt for large scale application.

  10. Separation, Identification, and Antidiabetic Activity of Catechin Isolated from Arbutus unedo L. Plant Roots.

    Science.gov (United States)

    Mrabti, Hanae Naceiri; Jaradat, Nidal; Fichtali, Ismail; Ouedrhiri, Wessal; Jodeh, Shehdeh; Ayesh, Samar; Cherrah, Yahia; Faouzi, My El Abbes

    2018-04-12

    Phytopharmaceuticals play an essential role in medicine, since the need to investigate highly effective and safe drugs for the treatment of diabetes mellitus disease remains a significant challenge for modern medicine. Arbutus unedo L. root has various therapeutic properties, and has been used widely in the traditional medicine as an antidiabetic agent. The current study aimed to isolate the pharmacologically active compound from A. unedo roots using accelerated solvent extraction technology, to determine its chemical structure using different instrumental analytical methods, and also to evaluate the α-glucosidase inhibitory activity. The roots of A. unedo were exhaustively extracted by high-pressure static extraction using the Zippertex ® technology (Dionex-ASE, Paris, France), and the extract was mixed with XAD-16 resin to reach quantifiable amounts of active compounds which were identified by high-pressure liquid chromatography (HPLC), ¹H NMR (300 MHz), and 13 C NMR. The antidiabetic activity of the isolated compound was evaluated using the α-glucosidase inhibitory assay. The active compound was isolated, and its structure was identified as catechin using instrumental analysis.The results revealed that the isolated compound has potential α-glucosidase inhibitory activity with an IC 50 value of 87.55 ± 2.23 μg/mL greater than acarbose. This was used as a positive control, which has an IC 50 value of 199.53 ± 1.12 μg/mL. According to the results achieved, the roots of A. unedo were considered the best source of catechin and the Zippertex ® technology method of extraction is the best method for isolation of this therapeutic active compound. In addition, the α-glucosidase inhibitory activity results confirmed the traditional use of A. unedo roots as an antidiabetic agent. Future clinical trials and investigations of antidiabetic and other pharmacological effects such as anticancer are required.

  11. Separation, Identification, and Antidiabetic Activity of Catechin Isolated from Arbutus unedo L. Plant Roots

    Directory of Open Access Journals (Sweden)

    Hanae Naceiri Mrabti

    2018-04-01

    Full Text Available Phytopharmaceuticals play an essential role in medicine, since the need to investigate highly effective and safe drugs for the treatment of diabetes mellitus disease remains a significant challenge for modern medicine. Arbutus unedo L. root has various therapeutic properties, and has been used widely in the traditional medicine as an antidiabetic agent. The current study aimed to isolate the pharmacologically active compound from A. unedo roots using accelerated solvent extraction technology, to determine its chemical structure using different instrumental analytical methods, and also to evaluate the α-glucosidase inhibitory activity. The roots of A. unedo were exhaustively extracted by high-pressure static extraction using the Zippertex® technology (Dionex-ASE, Paris, France, and the extract was mixed with XAD-16 resin to reach quantifiable amounts of active compounds which were identified by high-pressure liquid chromatography (HPLC, 1H NMR (300 MHz, and 13C NMR. The antidiabetic activity of the isolated compound was evaluated using the α-glucosidase inhibitory assay. The active compound was isolated, and its structure was identified as catechin using instrumental analysis.The results revealed that the isolated compound has potential α-glucosidase inhibitory activity with an IC50 value of 87.55 ± 2.23 μg/mL greater than acarbose. This was used as a positive control, which has an IC50 value of 199.53 ± 1.12 μg/mL. According to the results achieved, the roots of A. unedo were considered the best source of catechin and the Zippertex® technology method of extraction is the best method for isolation of this therapeutic active compound. In addition, the α-glucosidase inhibitory activity results confirmed the traditional use of A. unedo roots as an antidiabetic agent. Future clinical trials and investigations of antidiabetic and other pharmacological effects such as anticancer are required.

  12. In Vitro Activities of Terbinafine against Cutaneous Isolates of Candida albicans and Other Pathogenic Yeasts

    Science.gov (United States)

    Ryder, Neil S.; Wagner, Sonja; Leitner, Ingrid

    1998-01-01

    Terbinafine is active in vitro against a wide range of pathogenic fungi, including dermatophytes, molds, dimorphic fungi, and some yeasts, but earlier studies indicated that the drug had little activity against Candida albicans. In contrast, clinical studies have shown topical and oral terbinafine to be active in cutaneous candidiasis and Candida nail infections. In order to define the anti-Candida activity of terbinafine, we tested the drug against 350 fresh clinical isolates and additional strains by using a broth dilution assay standardized according to the guidelines of the National Committee for Clinical Laboratory Standards (NCCLS) M27-A assay. Terbinafine was found to have an MIC of 1 μg/ml for reference C. albicans strains. For 259 clinical isolates, the MIC at which 50% of the isolates are inhibited (MIC50) of terbinafine was 1 μg/ml (fluconazole, 0.5 μg/ml), and the MIC90 was 4 μg/ml (fluconazole, 1 μg/ml). Terbinafine was highly active against Candida parapsilosis (MIC90, 0.125 μg/ml) and showed potentially interesting activity against isolates of Candida dubliniensis, Candida guilliermondii, Candida humicola, and Candida lusitaniae. It was not active against the Candida glabrata, Candida krusei, and Candida tropicalis isolates in this assay. Cryptococcus laurentii and Cryptococcus neoformans were highly susceptible to terbinafine, with MICs of 0.06 to 0.25 μg/ml. The NCCLS macrodilution assay provides reproducible in vitro data for terbinafine against Candida and other yeasts. The MICs for C. albicans and C. parapsilosis are compatible with the known clinical efficacy of terbinafine in cutaneous infections, while the clinical relevance of its activities against the other species has yet to be determined. PMID:9593126

  13. Tests and calculations on a scale one spent fuel storage rack

    International Nuclear Information System (INIS)

    Moudrik, R.; Queval, J.C.; Gantenbein, F.; Trollat, C.

    1995-01-01

    This paper deals with the behaviour of racks of PWR reactor (pressurized water reactor) set in a pool, in case of an earthquake. Several parameters are taken into account: fluid, loading, gaps,... The paper presents some tests that can be implemented on the racks, in order to valid them before use. Moreover, a program and the results are presented, as well as calculation. (TEC). 2 refs., 9 figs

  14. Surveillance of a Ventilated Rack System for Corynebacterium bovis by Sampling Exhaust-Air Manifolds.

    Science.gov (United States)

    Manuel, Christopher A; Pugazhenthi, Umarani; Leszczynski, Jori K

    2016-01-01

    Corynebacterium bovis causes an opportunistic infection of nude (Foxn1, nu/nu) mice, leading to nude mouse hyperkeratotic dermatitis (scaly skin disease). Enzootic in many nude mouse colonies, C. bovis spreads rapidly to naive nude mice, despite modern husbandry practices, and is very difficult to eradicate. To facilitate rapid detection in support of eradication efforts, we investigated a surveillance method based on quantitative real-time PCR (qPCR) evaluation of swabs collected from the horizontal exhaust manifold (HEM) of an IVC rack system. We first evaluated the efficacy of rack sanitation methods for removing C. bovis DNA from the HEM of racks housing endemic colonies of infected nude mice. Pressurized water used to flush the racks' air exhaust system followed by a standard rack-washer cycle was ineffective in eliminating C. bovis DNA. Only after autoclaving did all sanitized racks test negative for C. bovis DNA. We then measured the effects of stage of infection (early or established), cage density, and cage location on the rack on time-to-detection at the HEM. Stage of infection significantly affected time-to-detection, independent of cage location. Early infections required 7.3 ± 1.2 d whereas established infections required 1 ± 0 d for detection of C. bovis at the HEM. Cage density influenced the quantity of C. bovis DNA detected but not time-to-detection. The location of the cage on the rack affected the time-to-detection only during early C. bovis infections. We suggest that qPCR swabs of HEM are useful during the routine surveillance of nude mouse colonies for C. bovis infection.

  15. Voluntary locomotor activity mitigates oxidative damage associated with isolation stress in the prairie vole (Microtus ochrogaster).

    Science.gov (United States)

    Fletcher, Kelsey L; Whitley, Brittany N; Treidel, Lisa A; Thompson, David; Williams, Annie; Noguera, Jose C; Stevenson, Jennie R; Haussmann, Mark F

    2015-07-01

    Organismal performance directly depends on an individual's ability to cope with a wide array of physiological challenges. For social animals, social isolation is a stressor that has been shown to increase oxidative stress. Another physiological challenge, routine locomotor activity, has been found to decrease oxidative stress levels. Because we currently do not have a good understanding of how diverse physiological systems like stress and locomotion interact to affect oxidative balance, we studied this interaction in the prairie vole (Microtus ochrogaster). Voles were either pair housed or isolated and within the isolation group, voles either had access to a moving wheel or a stationary wheel. We found that chronic periodic isolation caused increased levels of oxidative stress. However, within the vole group that was able to run voluntarily, longer durations of locomotor activity were associated with less oxidative stress. Our work suggests that individuals who demonstrate increased locomotor activity may be better able to cope with the social stressor of isolation. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

  16. Association between physical activity, participation in Physical Education classes, and social isolation in adolescents.

    Science.gov (United States)

    Santos, Simone José dos; Hardman, Carla Menêses; Barros, Simone Storino Honda; Santos da Franca, Carolina; Santos, Carolina da F B F; Barros, Mauro Virgilio Gomes de

    2015-01-01

    To analyze the association between physical activity, participation in Physical Education classes, and indicators of social isolation among adolescents. This was an epidemiological study based on secondary analysis of data from a representative sample of students (14-19 years) from public high schools (n=4,207). Data were collected through the questionnaire Global School-based Student Health Survey. The independent variables were the level of physical activity and enrollment in Physical Education classes, while the dependent variables were two indicators of social isolation (feeling of loneliness and having few friends). Descriptive and inferential procedures were used in the statistical analysis. Most of the adolescents were classified as insufficiently active (65.1%) and reported not attending Physical Education classes (64.9%). Approximately two in each ten participants reported feeling of loneliness (15.8%) and, in addition, about one in each five adolescents reported have only one friend (19.5%). In the bivariate analysis, a significantly lower proportion of individuals reporting social isolation was observed among adolescents who referred higher enrollment in Physical Education classes. After adjustment for confounding variables, binary logistic regression showed that attending Physical Education classes was identified as a protective factor in relation to the indicator of social isolation 'having few friends,' but only for girls. It was concluded that participation in Physical Education classes is associated with reduced social isolation among female adolescents. Copyright © 2015 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. All rights reserved.

  17. Antagonistic Activity of Nocardia brasiliensis PTCC 1422 Against Isolated Enterobacteriaceae from Urinary Tract Infections.

    Science.gov (United States)

    Jalali, Hossnieh Kafshdar; Salamatzadeh, Abdolreza; Jalali, Arezou Kafshdar; Kashani, Hamed Haddad; Asbchin, Salman Ahmadi; Issazadeh, Khosro

    2016-03-01

    The main drawback of current antibiotic therapies is the emergence and rapid increase in antibiotic resistance. Nocardiae are aerobic, Gram-positive, catalase-positive, non-motile actinomycetes. Nocardia brasiliensis was reported as antibiotic producer. The purpose of the study was to determine antibacterial activity of N. brasiliensis PTCC 1422 against isolated Enterobacteriaceae from urinary tract infections (UTIs). The common bacteria from UTIs were isolated from hospital samples. Antimicrobial susceptibility test was performed for the isolated pathogens using Kirby-Bauer disk diffusion method according to clinical and Laboratory Standards Institute guideline. Antagonistic activity of N. brasiliensis PTCC 1422 was examined with well diffusion methods. Supernatant of N. brasiliensis PTCC 1422 by submerged culture was analyzed with gas chromatography-mass spectrometry. Isolated strains included Escherichia coli, Klebsiella pneumoniae, Serratia marcescens and Proteus mirabilis. The most common pathogen isolated was E. coli (72.5%). Bacterial isolates revealed the presence of high levels of antimicrobial resistances to ceftriaxone and low levels of resistance to cephalexin. Supernatant of N. brasiliensis PTCC 1422 showed antibacterial activity against all of the isolated microorganisms in well diffusion method. The antibiotic resistance among the uropathogens is an evolving process, so a routine surveillance to monitor the etiologic agents of UTI and the resistance pattern should be carried out timely to choose the most effective empirical treatment by the physicians. Our present investigation indicates that the substances present in the N. brasiliensis PTCC 1422 could be used to inhibit the growth of human pathogen. Antibacterial resistance among bacterial uropathogen is an evolving process. Therefore, in the field on the need of re-evaluation of empirical treatment of UTIs, our present. The study has demonstrated that N. brasiliensis PTCC 1422 has a high potential

  18. Antioxidant activity of probiotic lactic acid bacteria isolated from Mongolian airag

    Directory of Open Access Journals (Sweden)

    E Uugantsetseg

    2014-12-01

    Full Text Available This research aimed to determine the antioxidant activity of probiotic lactic acid bacteria isolated from airag. In this study, 42 lactic acid bacteria were isolated from Mongolian airag. All isolates were identified by using morphological, biochemical and physiological methods. The isolated bacteria were studied for antagonistic effects on Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, 22 strains showed antibacterial activity. When we examined their probiotic properties such as bile acid tolerance and gastric acid tolerance, it is shown that only 6 bacterial strains can survive up to 3  hours in a pH 3.0 acid environment  and up to 8 hours in  0.3% bile acid environment. Selected probiotic strains were further identified to species by API 50CHL system. Antioxidant activity of  probiotic  strains were determined by 1,1-diphenyl-2 picrylhydrazyl (DPPH assay. While the antioxidant activity in cell free supernatant fluctuated between the range of 26.1-38.4%,  the antioxidant activity after 72 hours of fermentation in the whey fraction was between 17.23-55.12%. DOI: http://doi.dx.org/10.5564/mjc.v15i0.327 Mongolian Journal of Chemistry 15 (41, 2014, p73-78

  19. Manufacturing and Construction of Fresh Fuel Storage Rack for a Research Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Jinho; Lee, Sangjin; Lee, Jongmin; Ryu, Jeong-Soo [KAERI, Daejeon (Korea, Republic of)

    2016-05-15

    The objective of this study is to provide the manufacturing and construction information regarding the Fresh Fuel Storage Rack (FFSR). The main function of a FFSR is to store and protect the 2 core new fuel assemblies for the operation of the research reactor. The fresh fuel assemblies are stored in a rack made of stainless steel and the storage rack is installed in the fresh fuel storage room. The fresh fuel facility provides fresh fuel assemblies with dry storage space. General design requirements of the fresh fuel storage facilities are given in the ANSI-57.3. Design, manufacturing, and construction of the fresh fuel storage rack are introduced. The analysis is performed to confirm the structural intensity of the fresh fuel storage rack under the seismic loads. The fresh fuel storage rack designed for storage of fresh fuel assemblies should be manufactured and installed with consideration of predicted number of fresh fuel assemblies, structural integrity, resistivity to corrosion and radiation, cleaning, and workability.

  20. A water-cooling solution for PC-racks of the LHC experiments

    CERN Document Server

    Vannerem, P

    2004-01-01

    With ever increasing power consumption and heat dissipation of todays CPUs, cooling of rack-mounted PCs is an issue for the future online farms of the LHC experiments. In order to investigate the viability of a water-cooling solution, a prototype PC-farm rack has been equipped with a commercially available retrofitted heat exchanger. The project has been carried out as a collaboration of the four LHC experiments and the PH-ESS group . This note reports on the results of a series of cooling and power measurements of the prototype rack with configurations of 30 to 48 PCs. The cooling performance of the rack-cooler is found to be adequate; it extracts the heat dissipated by the CPUs efficiently into the cooling water. Hence, the closed PC rack transfers almost no heat into the room. The measurements and the failure tests show that the rack-cooler concept is a viable solution for the future PC farms of the LHC experiments.

  1. Manufacturing and Construction of Fresh Fuel Storage Rack for a Research Reactor

    International Nuclear Information System (INIS)

    Oh, Jinho; Lee, Sangjin; Lee, Jongmin; Ryu, Jeong-Soo

    2016-01-01

    The objective of this study is to provide the manufacturing and construction information regarding the Fresh Fuel Storage Rack (FFSR). The main function of a FFSR is to store and protect the 2 core new fuel assemblies for the operation of the research reactor. The fresh fuel assemblies are stored in a rack made of stainless steel and the storage rack is installed in the fresh fuel storage room. The fresh fuel facility provides fresh fuel assemblies with dry storage space. General design requirements of the fresh fuel storage facilities are given in the ANSI-57.3. Design, manufacturing, and construction of the fresh fuel storage rack are introduced. The analysis is performed to confirm the structural intensity of the fresh fuel storage rack under the seismic loads. The fresh fuel storage rack designed for storage of fresh fuel assemblies should be manufactured and installed with consideration of predicted number of fresh fuel assemblies, structural integrity, resistivity to corrosion and radiation, cleaning, and workability

  2. Test Rack Development for Extended Operation of Advanced Stirling Convertors at NASA Glenn Research Center

    Science.gov (United States)

    Dugala, Gina M.

    2010-01-01

    The U.S. Department of Energy, Lockheed Martin Space Systems Company, Sunpower Inc., and NASA Glenn Research Center (GRC) have been developing an Advanced Stirling Radioisotope Generator (ASRG) for use as a power system on space science missions. This generator will make use of free-piston Stirling convertors to achieve higher conversion efficiency than with currently available alternatives. One part of NASA GRC's support of ASRG development includes extended operation testing of Advanced Stirling Convertors (ASCs) developed by Sunpower Inc. and GRC. The ASC consists of a free-piston Stirling engine integrated with a linear alternator. NASA GRC has been building test facilities to support extended operation of the ASCs for several years. Operation of the convertors in the test facility provides convertor performance data over an extended period of time. One part of the test facility is the test rack, which provides a means for data collection, convertor control, and safe operation. Over the years, the test rack requirements have changed. The initial ASC test rack utilized an alternating-current (AC) bus for convertor control; the ASRG Engineering Unit (EU) test rack can operate with AC bus control or with an ASC Control Unit (ACU). A new test rack is being developed to support extended operation of the ASC-E2s with higher standards of documentation, component selection, and assembly practices. This paper discusses the differences among the ASC, ASRG EU, and ASC-E2 test racks.

  3. A Study on Rack Thickness Effect for Spent Fuel Pool Storage

    International Nuclear Information System (INIS)

    Kim, Mi Jin; Lee, Hee-Jae; Sohn, Dong-Seong

    2015-01-01

    For the effective storage of used fuel, the development of high performance neutron absorbing materials is needed. One of the major concern for the used fuel storage is the assurance to keep subcriticality of the storage rack and the high performance neutron absorbing material is the vital part to assure this requirement. According to NRC guide line, the k-effective of the spent fuel storage racks must not exceed 0.95. To ensure its safety, subcriticality analysis is required. Subcriticality analysis of the used storage in spent fuel pool have been performed by different authors. Criticality calculations for light water reactor spent fuel storage rack were carried out by Jae et al. They used AMPX-KENO IV code and considered the effect of rack pitch and rack thickness for consolidated fuel. The criticality analysis has performed at Gd 0.2 and 1 wt% according to thickness change. As thickness increases, the volume of the spent fuel pool rack increases. Therefore, absorbing material also increases according to thickness

  4. A Study on Rack Thickness Effect for Spent Fuel Pool Storage

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Mi Jin; Lee, Hee-Jae; Sohn, Dong-Seong [Ulsan National Institute of Science and Technology, Ulsan (Korea, Republic of)

    2015-05-15

    For the effective storage of used fuel, the development of high performance neutron absorbing materials is needed. One of the major concern for the used fuel storage is the assurance to keep subcriticality of the storage rack and the high performance neutron absorbing material is the vital part to assure this requirement. According to NRC guide line, the k-effective of the spent fuel storage racks must not exceed 0.95. To ensure its safety, subcriticality analysis is required. Subcriticality analysis of the used storage in spent fuel pool have been performed by different authors. Criticality calculations for light water reactor spent fuel storage rack were carried out by Jae et al. They used AMPX-KENO IV code and considered the effect of rack pitch and rack thickness for consolidated fuel. The criticality analysis has performed at Gd 0.2 and 1 wt% according to thickness change. As thickness increases, the volume of the spent fuel pool rack increases. Therefore, absorbing material also increases according to thickness.

  5. Antibacterial assay-guided isolation of active compounds from Artocarpus heterophyllus heartwoods.

    Science.gov (United States)

    Septama, Abdi Wira; Panichayupakaranant, Pharkphoom

    2015-01-01

    Preparations from Artocarpus heterophyllus Lam. (Moraceae) heartwoods are used in the traditional folk medicine for the treatment of inflammation, malarial fever, and to prevent bacterial and fungal infections. The objective of this study was to isolate pure antibacterial compounds from A. heterophyllus heartwoods. The dried and powdered A. heterophyllus heartwoods were successively extracted with the following solvents: hexane, ethyl acetate, and methanol. Each of the extracts was screened for their antibacterial activities using a disc diffusion method (10 mg/disc). Their minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined using a broth microdilution method. The extract that showed the strongest antibacterial activities was fractionated to isolate the active compounds by an antibacterial assay-guided isolation process. The ethyl acetate extract exhibited the strongest antibacterial activities against Streptococcus mutans, S. pyogenes, and Bacillus subtilis with MIC values of 78, 39, and 9.8 µg/mL, respectively. Based on an antibacterial assay-guided isolation, four antibacterial compounds: cycloartocarpin (1), artocarpin (2), artocarpanone (3), and cyanomaclurin (4) were purified. Among these isolated compounds, artocarpin exhibited the strongest antibacterial activity against Gram-positive bacteria, including S. mutans, S. pyogenes, B. subtilis, Staphylococcus aureus, and S. epidermidis with MICs of 4.4, 4.4, 17.8, 8.9, and 8.9 µM, respectively, and MBCs of 8.9, 8.9, 17.8, 8.9, and 8.9 µM, respectively, while artocarpanone showed the strongest activity against Escherichia coli, a Gram-negative bacteria with MIC and MBC values of 12.9 and 25.8 µM, respectively. Only artocarpin showed inhibitory activity against Pseudomonas aeruginosa with an MIC of 286.4 µM.

  6. Stability Analysis for Isolated AC Microgrids Based on PV-Active Generators

    DEFF Research Database (Denmark)

    Aldana, Nelson Leonardo Diaz; Coelho, Ernane A. A.; Quintero, Juan Carlos Vasquez

    2015-01-01

    The current trend in isolated microgrids is oriented to distributed renewable energy generators, such as photovoltaic (PV) generators and their corresponding distributed energy storage systems (ESS) as an unit denoted as active generator (PV+ESS). In an isolated microgrid, every distributed...... for all the distributed generators. In particular, ESS’s based on batteries require at least two different mode of charge. As consequence, the topological operation mode of the microgrid is affected by the changes of the operation mode of each distributed generator. Typically, droop control loops are used...... for interconnecting several different distributed generators in parallel to a common bus, whose parameters determine the stability and damping of the microgrid operation. In this paper, a small-signal stability analysis is applied to an isolated AC microgrid composed of (PV+ESS) active generators, regarding three...

  7. The antagonistic activity of lactic acid bacteria isolated from peda, an Indonesian traditional fermented fish

    Science.gov (United States)

    Putra, T. F.; Suprapto, H.; Tjahjaningsih, W.; Pramono, H.

    2018-04-01

    Peda is an Indonesian traditional fermented whole fish prepared by addition of salt prior to fermentation and drying process. Salt used to control the growth of the lactic acid bacteria for the fermentation process. The objectives of this study were isolating and characterize the potential lactic acid bacteria (LAB) from peda as culture starter candidate, particularly its activity against pathogenic bacteria. A total of five samples from five regions of East Java Province was collected and subjected to LAB isolation. Fifty-seven of 108 colonies that show clear zone in de Man, Rogosa and Sharpe (MRS) agar supplemented with 0.5% CaCO3 were identified as LAB. Twenty-seven of the LAB isolates were exhibit inhibition against Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 27853. Isolate Aerococcus NJ-20 was exhibited strong inhibition against S. aureus ATCC 6538 (7.6 ± 1.35 mm inhibition zone) but was not produce bacteriocin. This finding suggests that the isolate Aerococcus NJ-20 can be applied as biopreservative culture starter on peda production. Further analysis on technological properties of isolates will be needed prior to application.

  8. Spatial Rack Drives Pitch Configurations: Essence and Content

    Science.gov (United States)

    Abadjieva, Emilia; Abadjiev, Valentin; Naganawa, Akihiro

    2018-03-01

    The practical realization of all types of mechanical motions converters is preceded by solving the task of their kinematic synthesis. In this way, the determination of the optimal values of the constant geometrical parameters of the chosen structure of the created mechanical system is achieved. The searched result is a guarantee of the preliminary defined kinematic characteristics of the synthesized transmission and in the first place, to guarantee the law of motions transformation. The kinematic synthesis of mechanical transmissions is based on adequate mathematical modelling of the process of motions transformation and on the object, realizing this transformation. Basic primitives of the mathematical models for synthesis upon a pitch contact point are geometric and kinematic pitch configurations. Their dimensions and mutual position in space are the input parameters for the processes of design and elaboration of the synthesized mechanical device. The study presented here is a brief review of the theory of pitch configurations. It is an independent scientific branch of the spatial gearing theory (theory of hyperboloid gears). On this basis, the essence and content of the corresponding primitives, applicable to the synthesis of spatial rack drives, are defined.

  9. Smart Rack Monitor for the Linac control system

    International Nuclear Information System (INIS)

    Shtirbu, S.; Goodwin, R.W.; McCrory, E.S.; Shea, M.F.

    1991-01-01

    The Smart Rack Monitor (SRM) is a low-cost, one board, data-acquisition module for the upgraded Linac control system at Fermilab. The SRM is based on the Motorola MC68332 microcontroller in the Business Card Computer (BCC) configuration. It is connected to the Linac local control station (to be referred to as Local Station) by an Arcnet LAN, and can be located close to the controlled hardware. Each Local Station is connected to several SRMs. The SRM has 64 A-D channels, sixteen D-A channels, and eight bytes of digital I/O on the mother board. Software Components Group's pSOS is the SRM's kernel. The SRM's software is cross-developed on VAX/VMS in C. The SRM does not have an attached console and is fully controlled by the Local Station. It performs data acquisition and settings, as directed by the Local Station. Its existence is transparent to the rest of the control system. The SRM supports code updates downloading from the VAX, through the Local Station

  10. Self-tuning MIMO disturbance feedforward control for active hard-mounted vibration isolators

    NARCIS (Netherlands)

    Beijen, M.A.; Heertjes, M.F.; Van Dijk, J.; Hakvoort, W. B.J.

    2018-01-01

    © 2017 Elsevier Ltd This paper proposes a multi-input multi-output (MIMO) disturbance feedforward controller to improve the rejection of floor vibrations in active vibration isolation systems for high-precision machinery. To minimize loss of performance due to model uncertainties, the feedforward

  11. Self-tuning MIMO disturbance feedforward control for active hard-mounted vibration isolators

    NARCIS (Netherlands)

    Beijen, M.A.; Heertjes, M.F.; van Dijk, J.W.; Hakvoort, W.B.J.

    2018-01-01

    This paper proposes a multi-input multi-output (MIMO) disturbance feedforward controller to improve the rejection of floor vibrations in active vibration isolation systems for high-precision machinery. To minimize loss of performance due to model uncertainties, the feedforward controller is

  12. Active and passive vibration isolation in piezoelectric phononic rods with external voltage excitation

    Directory of Open Access Journals (Sweden)

    Qicheng Zhang

    2017-05-01

    Full Text Available Active piezoelectric materials are applied to one-dimensional phononic crystals, for the control of longitudinal vibration propagation both in active and passive modes. Based on the electromechanical coupling between the acoustical vibration and electric field, the electromechanical equivalent method is taken to theoretically predict the transmission spectrum of the longitudinal vibration. It is shown that the phononic rod can suppress the vibration efficiently at the frequencies of interest, by actively optimizing the motions of piezoelectric elements. In an illustrated phononic rod of 11.2cm long, active tunable isolations of more than 20dB at low frequencies (500Hz-14kHz are generated by controlling the excitation voltages of piezoelectric elements. Meanwhile, passive fixed isolation at high frequencies (14k-63kHz are presented by its periodicity characteristics. Finite element simulations and vibration experiments on the rod demonstrate the effectiveness of the approach in terms of its vibration isolation capabilities and tunable characteristics. This phononic rod can be manufactured easily and provides numerous potential applications in designing isolation mounts and platforms.

  13. Isolation of Mucorales from processed maize (Zea mays L.) and screening for protease activity

    Science.gov (United States)

    de Azevedo Santiago, André Luiz Cabral Monteiro; de Souza Motta, Cristina Maria

    2008-01-01

    Mucorales were isolated from maize flour, corn meal and cooked cornflakes using surface and depth plate methods. Rhizopus oryzae, Circinella muscae, Mucor subtilissimus, Mucor hiemalis f. hiemalis, Syncephalastrum racemosum, Rhizopus microsporus var. chinensis and Absidia cylindrospora showed protease activity. PMID:24031292

  14. Anti-bacterial activity of Extract of Crinum jagus bulb against Isolates ...

    African Journals Online (AJOL)

    Crinum jagus plant has been reportedly used for treatment of infectious diseases in Nigeria. In this study, the antibacterial activity of the crude extract and chromatographic fractions from the bulb of Crinium jagus against Mycobacterium tuberculosis isolates was investigated using Lowenstein-Jensen medium (LJ) and ...

  15. Antioxidant and Antiplasmodial Activities of Bergenin and 11-O-Galloylbergenin Isolated from Mallotus philippensis

    Science.gov (United States)

    Khan, Hamayun; Amin, Hazrat; Ullah, Asad; Saba, Sumbal; Rafique, Jamal; Khan, Khalid; Ahmad, Nasir; Badshah, Syed Lal

    2016-01-01

    Two important biologically active compounds were isolated from Mallotus philippensis. The isolated compounds were characterized using spectroanalytical techniques and found to be bergenin (1) and 11-O-galloylbergenin (2). The in vitro antioxidant and antiplasmodial activities of the isolated compounds were determined. For the antioxidant potential, three standard analytical protocols, namely, DPPH radical scavenging activity (RSA), reducing power assay (RPA), and total antioxidant capacity (TAC) assay, were adopted. The results showed that compound 2 was found to be more potent antioxidant as compared to 1. Fascinatingly, compound 2 displayed better EC50 results as compared to α-tocopherol while being comparable with ascorbic acid. The antiplasmodial assay data showed that both the compound exhibited good activity against chloroquine sensitive strain of Plasmodium falciparum (D10) and IC50 values were found to be less than 8 μM. The in silico molecular docking analyses were also performed for the determination of binding affinity of the isolated compounds using P. falciparum proteins PfLDH and Pfg27. The results showed that compound 2 has high docking score and binding affinity to both protein receptors as compared to compound 1. The demonstrated biological potentials declared that compound 2 could be the better natural antioxidant and antiplasmodial candidate. PMID:26998192

  16. Antioxidant and Antiplasmodial Activities of Bergenin and 11-O-Galloylbergenin Isolated from Mallotus philippensis

    Directory of Open Access Journals (Sweden)

    Hamayun Khan

    2016-01-01

    Full Text Available Two important biologically active compounds were isolated from Mallotus philippensis. The isolated compounds were characterized using spectroanalytical techniques and found to be bergenin (1 and 11-O-galloylbergenin (2. The in vitro antioxidant and antiplasmodial activities of the isolated compounds were determined. For the antioxidant potential, three standard analytical protocols, namely, DPPH radical scavenging activity (RSA, reducing power assay (RPA, and total antioxidant capacity (TAC assay, were adopted. The results showed that compound 2 was found to be more potent antioxidant as compared to 1. Fascinatingly, compound 2 displayed better EC50 results as compared to α-tocopherol while being comparable with ascorbic acid. The antiplasmodial assay data showed that both the compound exhibited good activity against chloroquine sensitive strain of Plasmodium falciparum (D10 and IC50 values were found to be less than 8 μM. The in silico molecular docking analyses were also performed for the determination of binding affinity of the isolated compounds using P. falciparum proteins PfLDH and Pfg27. The results showed that compound 2 has high docking score and binding affinity to both protein receptors as compared to compound 1. The demonstrated biological potentials declared that compound 2 could be the better natural antioxidant and antiplasmodial candidate.

  17. Molecular Analysis, Biochemical Characterization, Antimicrobial Activity, and Immunological Analysis of Proteus mirabilis Isolated from Broilers.

    Science.gov (United States)

    Yeh, Hung-Yueh; Line, John E; Hinton, Arthur

    2018-03-01

    Proteus mirabilis, a Gram-negative bacterium, is ubiquitous in the environment and is considered as the normal microflora in the human gastrointestinal tract. However, this bacterium is an opportunistic pathogen in humans, often causing urinary tract infections. Moreover, Proteus has been frequently isolated from food animals, including poultry. Whether this bacterium contributes to the foodborne illness in humans is unclear. In this report, P. mirabilis isolates recovered from broilers during housing in the units were characterized, their antimicrobial activity was assayed, and broiler immune response to the soluble proteins was determined. Cecal contents and fecal droppings were treated according to the standard protocol for isolation. Speciation based on biochemical reactions and the antimicrobial activity of the isolates were carried out using commercial kits. Immunoblot was assayed to determine immune status of broilers against P. mirabilis. A total of 10 isolates of P. mirabilis were selected for further characterization. These isolates could grow in pH 6.0 and 1% NaCl conditions. They were resistant to sodium lactate, troleandomycin, rifamycin SV, vancomycin, but sensitive to nalidixic acid, cefotaxime and novobiocin. Moreover, the CTX, ACC, CMY-1, BIC, NDM, VEB, qnrB and qnrD genes were detected by PCR amplification in all isolates. Sera from broilers harboring this bacterium reacted to the P. mirabilis soluble proteins, but not from litter- and age-matched P. mirabilis negative and SPF chickens, indicating that this bacterium infected chickens that could have humoral immune response against P. mirabilis. This study provides a rationale for further monitoring P. mirabilis during poultry production to determine whether this bacterium poses potential threats to public health. Published 2018. This article is a U.S. Government work and is in the public domain in the USA.

  18. In vitro differential activity of phospholipases and acid proteinases of clinical isolates of Candida

    Directory of Open Access Journals (Sweden)

    Aurean D'Eça Júnior

    2011-06-01

    Full Text Available INTRODUCTION: Candida yeasts are commensals; however, if the balance of normal flora is disrupted or the immune defenses are compromised, Candida species can cause disease manifestations. Several attributes contribute to the virulence and pathogenicity of Candida, including the production of extracellular hydrolytic enzymes, particularly phospholipase and proteinase. This study aimed to investigate the in vitro activity of phospholipases and acid proteinases in clinical isolates of Candida spp. METHODS: Eighty-two isolates from hospitalized patients collected from various sites of origin were analyzed. Phospholipase production was performed in egg yolk medium and the production of proteinase was verified in a medium containing bovine serum albumin. The study was performed in triplicate. RESULTS: Fifty-six (68.3% of isolates tested were phospholipase positive and 16 (44.4% were positive for proteinase activity. C. tropicalis was the species with the highest number of positive isolates for phospholipase (91.7%. Statistically significant differences were observed in relation to production of phospholipases among species (p<0,0001 and among the strains from different sites of origin (p=0.014. Regarding the production of acid protease, the isolates of C. parapsilosis tested presented a larger number of producers (69.2%. Among the species analyzed, the percentage of protease producing isolates did not differ statistically (χ2=1.9 p=0.5901 (χ2=1.9 p=0.5901. CONCLUSIONS: The majority of C. non-albicans and all C. albicans isolates were great producers of hydrolytic enzymes and, consequently, might be able to cause infection under favorable conditions.

  19. IDENTIFICATION AND BIOLOGICAL ACTIVITY OF POTENTIAL PROBIOTIC BACTERIA ISOLATED FROM POECILIA RETICULATA (GUPPY

    Directory of Open Access Journals (Sweden)

    Aparna Balakrishna

    2014-08-01

    Full Text Available Antagonistic activities against candidate indicator strains, adhesion to mucus and biofilm formation of potential probiotic strains isolated from Poecilia reticulata were evaluated. Four isolated strains (MBTU_PB1, MBTU_PB2, MBTU_PB3 and MBTU_PB4 showed moderate to strong antagonistic activities against the tested five indicator strains (Aeromonas hydrophila1739, Vibrio cholera 3906, Flavobacterium 2495, Acinetobacter 1271 and Alcaligenes 1424 and these isolates were further identified using biochemical tests and 16S rDNA gene sequence analysis. Except the whole cell product, the other three cellular components, namely, heat-killed whole cell product, intracellular product and extracellular product of all the four selected isolates were equally effective, as revealed by the zone of inhibitions to the tested indicator strains. The in vitro adhesion property or the ability of colonization is often considered as a selection criteria for probiotics. All the selected four strains had higher adhesion abilities than the indicator strains. Further, these four strains had the ability to form biofilms on polystyrene surfaces. The in vitro characterization of these four strains suggests possibility of using the isolates, as individual strain or in combination, for probiotic therapy in aquaculture.

  20. Parametric Design and Multiobjective Optimization of Maglev Actuators for Active Vibration Isolation System

    Directory of Open Access Journals (Sweden)

    Qianqian Wu

    2014-05-01

    Full Text Available The microvibration has a serious impact on science experiments on the space station and on image quality of high resolution satellites. As an important component of the active vibration isolation platform, the maglev actuator has a large stroke and exhibits excellent isolating performance benefiting from its noncontact characteristic. A maglev actuator with good linearity was designed in this paper. Fundamental features of the maglev actuator were obtained by finite element simulation. In order to minimize the coil weight and the heat dissipation of the maglev actuator, parametric design was carried out and multiobjective optimization based on the genetic algorithm was adopted. The optimized actuator has better mechanical properties than the initial one. Active vibration isolation platforms for different-scale payload were designed by changing the arrangement of the maglev actuators. The prototype to isolate vibration for small-scale payload was manufactured and the experiments for verifying the characteristics of the actuators were set up. The linearity of the actuator and the mechanical dynamic response of the vibration isolation platform were obtained. The experimental results highlight the effectiveness of the proposed design.

  1. Multiple, novel biologically active endophytic actinomycetes isolated from upper Amazonian rainforests.

    Science.gov (United States)

    Bascom-Slack, Carol A; Ma, Cong; Moore, Emily; Babbs, Beatrice; Fenn, Kathleen; Greene, Joshua S; Hann, Bradley D; Keehner, Jocelyn; Kelley-Swift, Elizabeth G; Kembaiyan, Vivek; Lee, Sun Jin; Li, Puyao; Light, David Y; Lin, Emily H; Schorn, Michelle A; Vekhter, Daniel; Boulanger, Lori-Ann; Hess, W M; Vargas, Percy Núñez; Strobel, Gary A; Strobel, Scott A

    2009-08-01

    Microbial biodiversity provides an increasingly important source of medically and industrially useful compounds. We have isolated 14 actinomycete species from a collection of approximately 300 plant stem samples from the upper Amazonian rainforest in Peru. All of the cultured isolates produce substances with inhibitory activity directed at a range of potential fungal and bacterial pathogens. For some organisms, this activity is very broad in spectrum while other organisms show specific activity against a limited number of organisms. Two of these organisms preferentially inhibit bacterial test organisms over eukaryotic organisms. rDNA sequence analysis indicates that these organisms are not equivalent to any other cultured deposits in GenBank. Our results provide evidence of the untapped biodiversity in the form of biologically active microbes present within the tissues of higher plants.

  2. Isolation, identification and anti-candidal activity of filamentous fungi from Saudi Arabia soil

    Directory of Open Access Journals (Sweden)

    Nouf M. Al-Enazi

    2018-02-01

    Full Text Available Ten fungal strains; namely, Penicillium melinii, Petriella setifera, Aspergillus pseudo-niger, Alternaria chlamydospora, Pythium nayoroense, Phoma glomerata, Mucor ramosissimus, Mucor racemosus, Fusarium chlamydosporum and Rhizopus azygosporus were isolated from soil. The extra- and intra-cellular extracts of the fungal strains grown on malt extract and yeast-extract sucrose media were screened for their anticandidal activity against different clinically-isolated Candida species. Most of the fungal extracts showed activity against different Candida species. However, the fungal strains grew on malt extract showed greater activities than those grew on yeast extract sucrose media. The activity of the intracellular was higher than the extracellular metabolites. All fungal extracts (extra and intra were similar in chemical constituent; they contained carbohydrates and/or glycosides, unsaturated sterols and/or triterpens, tannins and traces of coumarins. Alkaloids, flavonoids, saponins, anthraquinones and cardenolides were no detected. The intra-cellular extracts contained more compounds than the extra-cellular extracts.

  3. Phytase Activity of Lactic Acid Bacteria Isolated from Dairy and Pharmaceutical Probiotic Products

    Directory of Open Access Journals (Sweden)

    Zohreh Khodaii

    2013-01-01

    Full Text Available Phytate, the major storage form of phosphorus in plant seeds, can form insoluble complexes with minerals such as iron, zinc and calcium thus reducing their bioavailability. Phytase enzymes are often used to upgrade the nutritional quality of phytate-rich foods and feeds such as grains. The phytate-degrading activity of 43 lactic acid bacteria including isolates from commercial probiotic preparations, dairy products and type strains were measured. The phytate-degrading activity of bifidobacteria and lactobacillus isolates from pharmaceutical probiotics, dairy products and type strains were determined. The enzyme activity of probotic bacteria ranged between 1.1-5.4 mU and was strain not species specific. Phytase activity may thus be a useful additional attribute of probiotics to be used as food supplements.

  4. Tenofovir alafenamide demonstrates broad cross-genotype activity against wild-type HBV clinical isolates and maintains susceptibility to drug-resistant HBV isolates in vitro.

    Science.gov (United States)

    Liu, Yang; Miller, Michael D; Kitrinos, Kathryn M

    2017-03-01

    Tenofovir alafenamide (TAF) is a novel prodrug of tenofovir (TFV). This study evaluated the antiviral activity of TAF against wild-type genotype A-H HBV clinical isolates as well as adefovir-resistant, lamivudine-resistant, and entecavir-resistant HBV isolates. Full length HBV genomes or the polymerase/reverse transcriptase (pol/RT) region from treatment-naïve patients infected with HBV genotypes A-H were amplified and cloned into an expression vector under the control of a CMV promoter. In addition, 11 drug resistant HBV constructs were created by site-directed mutagenesis of a full length genotype D construct. Activity of TAF was measured by transfection of each construct into HepG2 cells and assessment of HBV DNA levels following treatment across a range of TAF concentrations. TAF activity in vitro was similar against wild-type genotype A-H HBV clinical isolates. All lamivudine- and entecavir-resistant isolates and 4/5 adefovir-resistant isolates were found to be sensitive to inhibition by TAF in vitro as compared to the wild-type isolate. The adefovir-resistant isolate rtA181V + rtN236T exhibited low-level reduced susceptibility to TAF. TAF is similarly active in vitro against wild-type genotype A-H HBV clinical isolates. The TAF sensitivity results for all drug-resistant isolates are consistent with what has been observed with the parent drug TFV. The in vitro cell-based HBV phenotyping assay results support the use of TAF in treatment of HBV infected subjects with diverse HBV genotypes, in both treatment-naive and treatment-experienced HBV infected patients. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. Antimicrobial activity and cytotoxicity of triterpenes isolated from leaves of Maytenus undata (Celastraceae).

    Science.gov (United States)

    Mokoka, Tsholofelo Abednego; McGaw, Lyndy Joy; Mdee, Ladislaus Kakore; Bagla, Victor Patrick; Iwalewa, Ezekiel Olugbenga; Eloff, Jacobus Nicolaas

    2013-05-20

    Plants of the genus Maytenus belong to the family Celastraceae and are widely used in folk medicine as anti-tumour, anti-asthmatic, analgesic, anti-inflammatory, antimicrobial and anti-ulcer agents, and as a treatment for stomach problems. The aim of this study was to isolate and identify active compounds with antifungal activity from Maytenus undata after a preliminary study highlighted promising activity in crude extracts. Sequential extracts of M. undata leaves prepared using hexane, dichloromethane (DCM), acetone and methanol (MeOH) were tested for activity against Cryptococcus neoformans, a fungal organism implicated in opportunistic infections. Bioassay-guided fractionation of the hexane extract using C. neoformans as test organism was carried out to isolate antifungal compounds. The cytotoxicity of compounds isolated in sufficient quantities was evaluated using a tetrazolium-based colorimetric cellular assay (MTT) and a haemagglutination assay (HA). The hexane extract was most active with an MIC of 20 μg/ml against C. neoformans. The triterpene compounds friedelin (1), epifriedelanol (2), taraxerol (3), 3-oxo-11α-methoxyolean-12-ene-30-oic acid (4), 3-oxo-11α-hydroxyolean-12-ene-30-oic acid (5) and 3,11-dihydroxyolean-12-ene-30-oic acid (6) were isolated. Compound 6 was isolated for the first time from a plant species. The antimicrobial activity of compounds 1, 3, 5 and 6 was determined against a range of bacteria and fungi implicated in opportunistic and nosocomial infections. Compounds 5 and 6 were the most active against all the tested microorganisms with MIC values ranging between 24 and 63 μg/ml, except against Staphylococcus aureus which was relatively resistant. Compounds 1 and 3 had a low toxicity with an LC50 > 200 μg/ml towards Vero cells in the MTT assay. Compounds 5 and 6 were toxic with LC50 values of 6.03±0.02 and 2.98±0.01 μg/ml, respectively. Compounds 1 and 3 similarly were not toxic to the red blood cells (RBCs) but compounds 5 and

  6. Fructanase and fructosyltransferase activity of non-Saccharomyces yeasts isolated from fermenting musts of Mezcal.

    Science.gov (United States)

    Arrizon, Javier; Morel, Sandrine; Gschaedler, Anne; Monsan, Pierre

    2012-04-01

    Fructanase and fructosyltransferase are interesting for the tequila process and prebiotics production (functional food industry). In this study, one hundred thirty non-Saccharomyces yeasts isolated from "Mezcal de Oaxaca" were screened for fructanase and fructosyltransferase activity. On solid medium, fifty isolates grew on Agave tequilana fructans (ATF), inulin or levan. In liquid media, inulin and ATF induced fructanase activities of between 0.02 and 0.27U/ml depending of yeast isolate. High fructanase activity on sucrose was observed for Kluyveromyces marxianus and Torulaspora delbrueckii, while the highest fructanase activity on inulin and ATF was observed for Issatchenkia orientalis, Cryptococcus albidus, and Candida apicola. Zygosaccharomyces bisporus and Candida boidinii had a high hydrolytic activity on levan. Sixteen yeasts belonging to K. marxianus, T. delbrueckii and C. apicola species were positive for fructosyltransferase activity. Mezcal microbiota proved to showed to be a source for new fructanase and fructosyltransferases with potential application in the tequila and food industry. Copyright © 2012 Elsevier Ltd. All rights reserved.

  7. Isolation of active constituents from cherry laurel (Laurocerasus officinalis Roem.) leaves through bioassay-guided procedures.

    Science.gov (United States)

    Akkol, Esra Küpeli; Kırmızıbekmez, Hasan; Küçükboyacı, Nurgün; Gören, Ahmet C; Yesilada, Erdem

    2012-01-31

    The fresh leaves of Laurocerasus officinalis Roem. (Rosaceae) are externally used against pain and feverish symptoms in Turkish folk medicine. Effects of the extracts, fractions and isolated compounds from the leaves of L. officinalis were investigated using in vivo models of inflammation and pain in mice. The crude ethanolic extract from the leaves of plant was sequentially fractionated into five subextracts; explicitly, n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol, and remaining water extracts. Further studies were carried out on the most active EtOAc subextract was further subjected to fractionation through column chromatography. For the anti-inflammatory activity, carrageenan-induced hind paw edema and acetic acid-induced increase in capillary permeability models, and for the antinociceptive activity p-benzoquinone-induced writhing test in mice were employed. Ethanolic extract of the leaves was shown to possess significant inhibitory activity in the assay methods without inducing any gastric damage. Through bioassay-guided fractionation and isolation procedures three phenolic compounds, 2-O-β-D-glucopyranosyl-2-hydroxyphenyl-acetic acid (1), kaempferol-3-O-β-D-xylopyranosyl-(1→2)-O-β-D-glucopyranoside (2) and (+)-catechin (3) were isolated from the active fraction and their structures were elucidated by spectral techniques (1D and 2D NMR, ESIMS). The experimental data verified that Laurocerasus officinalis leaves displayed remarkable anti-inflammatory and antinociceptive activity. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  8. Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae).

    Science.gov (United States)

    Katoch, Meenu; Singh, Gurpreet; Sharma, Sadhna; Gupta, Nidhi; Sangwan, Payare Lal; Saxena, Ajit Kumar

    2014-02-11

    Endophytes, which reside in plant tissues, have the potential to produce novel metabolites with immense benefits for health industry. Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell were investigated. Endophytic fungi were isolated from the Bacopa monnieri. Extracts from liquid cultures were tested for cytotoxicity against a number of cancer cell lines using the MTT assay. Antimicrobial activity was determined using the micro dilution method. 22% of the examined extracts showed potent (IC50 of <20 μg/ml) cytotoxic activity against HCT-116 cell line. 5.5%, 11%, 11% of the extracts were found to be cytotoxic for MCF-7, PC-3, and A-549 cell lines respectively. 33% extracts displayed antimicrobial activity against at least one test organism with MIC value 10-100 μg/ml. The isolate B9_Pink showed the most potent cytotoxic activity for all the cell lines examined and maximum antimicrobial activity against the four pathogens examined which was followed by B19. Results indicated the potential for production of bioactive agents from endophytes of Bacopa monnieri.

  9. Structure-antioxidant activity relationships of flavonoids isolated from the resinous exudate of Heliotropium sinuatum.

    Science.gov (United States)

    Modak, Brenda; Contreras, M Leonor; González-Nilo, Fernando; Torres, René

    2005-01-17

    Relationships between the structural characteristics of flavonoids isolated from the resinous exudate of Heliotropium sinuatum and their antioxidant activity were studied. Radical formation energies, DeltaH of dehydrogenation and spin densities were calculated using DFT methods (B3LYP/6-31G*). Results show that studied flavonoids can be divided into two sets according to their activity. It has been found that antioxidant activity depends both on substitution pattern of hydroxyl groups of the flavonoid skeleton and the presence of an unsaturation at the C2-C3 bond. A good tendency between DeltaH of dehydrogenation and antioxidant activity was established.

  10. Activity-guided isolation of cytotoxic bis-bibenzyl constituents from Dumortiera hirsuta.

    Science.gov (United States)

    Toyota, Masao; Ikeda, Risa; Kenmoku, Hiromichi; Asakawa, Yoshinori

    2013-01-01

    Activity-guided fractionation of the ether extract of Dumortiera hirsute (Japanese liverwort), using cytotoxicity testing with cultured HL 60 and KB cells, resulted in the isolation of a new cytotoxic bis-bibenzyl compound, along with the two known bis-bibenzyls: isomarchantin C and isoriccardin C. The structural determination of the new bis-bibenzyl through extensive NMR spectral data indicated a derivative of marchantin A, which has been isolated from the liverwort Marchantia polymorpha. The cytotoxicity of the bis-bibenzyls was evaluated by the MTT (3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay using cultured HL 60 and KB cells.

  11. An Overview of Neolignans of the Genus Piper L.: Isolation Methods and Biological Activities.

    Science.gov (United States)

    Macedo, Arthur Ladeira; Dos Santos, Thais Carvalho Costa; Valverde, Alessandra Leda; Moreira, Davyson de Lima; Vasconcelos, Thatyana Rocha Alves

    2017-01-01

    The genus Piper L. has the shikimic acid pathway predominantly expressed, biosynthesizing many cinnamic acid derivatives (CAD). Neolignans comprise an important class of CAD that exhibit a wide range of pharmacological properties such as antibacterial, antitumor, insecticidal, anti-inflammatory, antioxidant, smooth muscle relaxant, neuroprotective, antiprotozoal and against platelet aggregation factor. These substances have been extracted and isolated from Piper species using different technics. The present review aims to summarize extraction and isolation methods and biological activities of the different types of neolignans covering the period from 1968 to January 2016. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  12. Comparison of antioxidant, anticholinesterase, and antidiabetic activities of three curcuminoids isolated from Curcuma longa L.

    Science.gov (United States)

    Kalaycıoğlu, Zeynep; Gazioğlu, Işıl; Erim, F Bedia

    2017-12-01

    Antioxidant, anticholinesterase and antidiabetic activities of three curcuminoids isolated from the Curcuma longa were simultaneously tested and compared in this study. The highest antioxidant power was detected for curcumin with the applied methods. The drug potentials of curcuminoids for Alzheimer's disease were controlled. Bisdemethoxycurcumin (BDMC) showed substantial inhibitory activity. The activity of demethoxycurcumin (DMC) followed BDMC, whereas curcumin showed very little acetylcholinesterase inhibition activity. Antidiabetic activity of curcuminoids was evaluated by their α-glucosidase inhibitory activities. All curcuminoids show activities with decreasing order as BDMC > curcumin > DMC. The significant activities of BDMC compared to its isomers and examination of chemical structures of isomers might be a starting point in designing new drugs for Alzheimer's and Diabetes Mellitus.

  13. Isolation of microorganisms with chinitase, protease and keratinase activities from petroleum contaminated soils

    International Nuclear Information System (INIS)

    Cervantes-Gonzalez, E.; Rojas-Avelizapa, L.; Cruz-Camarillo, R.; Rojas-Avelizapa, N.G.

    2005-01-01

    The most important part in one process of bio-remediation are the microorganisms with the capacities to degrade target compounds, this research is based to find microorganisms hydrocarbon-clastic with enzyme activities to degrade chicken feather (keratinolytic activity) which is also a contaminant and has been used such as sorbent of petroleum and can be composted after the oil spill cleanup is complete, the isolation was also to degrade shrimp waste (chitinolitic and proteolitic activity) which is waste material that can be used in compost or such as sorbent of petroleum too. We isolated mesofilic aerobic microorganisms from mexican soils located in Tabasco, Mexico. We achieved to isolate 105 bacteria from 10 soils, 90% was Bacillus Gram (-) which are common in soils and all were hydrocarbon-clastic, only 7 different bacteria had protease and chitinase activity and 12 bacteria had keratinase activity. So we found three fungi and one actinomycete with capacity to degrade hydrocarbons and presence of chitinase activity. The results of growth and enzyme activities in liquid culture showed that the protease activity was produced between 18 and 48 h in almost all bacteria, the chitinase activity started at 12 h but was slight , only 0.5 U/ml, and the keratinase activity was produced after 6 h of incubation and there were correlation between logarithmic phase of growth and enzymes production. With this study we showed the existence of some enzyme activities from microorganisms that live in hostile habitats. This, can be useful in bio-treatment soils by the possible use of this type of residues that can be bio-degraded at the same time that the hydrocarbons increasing the speed or the quality of cleanup in soils. (authors)

  14. The in vitro isolated whole guinea pig brain as a model to study epileptiform activity patterns.

    Science.gov (United States)

    de Curtis, Marco; Librizzi, Laura; Uva, Laura

    2016-02-15

    Research on ictogenesis is based on the study of activity between seizures and during seizures in animal models of epilepsy (chronic condition) or in in vitro slices obtained from naïve non-epileptic brains after treatment with pro-convulsive drugs, manipulations of the extracellular medium and specific stimulation protocols. The in vitro isolated guinea pig brain retains the functional connectivity between brain structures and maintains interactions between neuronal, glial and vascular compartments. It is a close-to-in vivo preparation that offers experimental advantages not achieved with the use of other experimental models. Neurophysiological and imaging techniques can be utilized in this preparation to study brain activity during and between seizures induced by pharmacological or functional manipulations. Cellular and network determinants of interictal and ictal discharges that reproduce abnormal patterns observed in human focal epilepsies and the associated changes in extracellular ion and blood-brain permeability can be identified and analyzed in the isolated guinea pig brain. Ictal and interictal patterns recorded in in vitro slices may show substantial differences from seizure activity recorded in vivo due to slicing procedure itself. The isolated guinea pig brain maintained in vitro by arterial perfusion combines the typical facilitated access of in vitro preparations, that are difficult to approach during in vivo experiments, with the preservation of larger neuronal networks. The in vitro whole isolated guinea pig brain preparation offers an unique experimental model to study systemic and neurovascular changes during ictogenesis. Published by Elsevier B.V.

  15. Analysis of ICPP fuel storage rack inner tie and corner tie substructures

    Energy Technology Data Exchange (ETDEWEB)

    Nitzel, M.E.; Rahl, R.G.

    1996-01-01

    Finite element models were developed and analyses performed for the tie plate, inner tie block assembly, and corner tie block assembly of a 25 port fuel rack assembly designed for installation in Pool 1 of Building 666 at the Idaho Chemical Processing Plant. These models were specifically developed to investigate the adequacy of certain welds joining components of the fuel storage rack assembly. The work scope for the task was limited to an investigation of the stress levels in the subject subassemblies when subjected to seismic loads. Structural acceptance criteria used for the elastic calculations performed were as found in the overall rack design report as issued by the rack`s designer, Holtec International. Structural acceptance criteria used for the plastic calculations performed as part of this effort were as defined in Subsection NF and Appendix F of the ASME Boiler & Pressure Vessel Code. The results of the analyses will also apply to the 30 port fuel storage rack design that is also scheduled for installation in Pool 1 of ICPP 666. The results obtained from the analyses performed for this task indicate that the welds joining the inner tie block and corner tie block to the surrounding rack structure meet the acceptance criteria. Further, the structural members (plates and blocks) were also found to be within the allowable stress limits established by the acceptance criteria. The separate analysis performed on the inner tie plate confirmed the structural adequacy for both the inner tie plate, corner tie plate, and tie block bolts. The analysis results verified that the inner tie and corner tie block should be capable of transferring the expected seismic load without structural failure.

  16. ISOLATION AND LIGNOCELLULOLYTIC ACTIVITIES OF FIBER-DIGESTING BACTERIA FROM DIGESTIVE TRACT OF TERMITE (Cryptothermes sp.

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    B.I.M. Tampoebolon

    2015-09-01

    Full Text Available The objectives of this study were to obtain the fiber-digesting bacteria isolates from termitedigestive tract and to determine the optimum conditions of growth and production of cellulase, xylanaseand ligninase enzyme of isolate. The first study was conducted to isolate and select the fiber-digestingbacteria from the digestive tract of termites based on the highest activity of cellulolytic (S, xylanolytic(X and lignolytic (L. The second study was optimation of the growth conditions of bacteria and theenzyme production due to effect of rice straw substrate and nitrogen. The material used were dry woodtermites, rice straw, and culture medium. The design used was a completely randomized factorial design,in which the first factor was rice straw substrate (1, 2, and 3% W/V, while the second factor wasnitrogen (0.1, 0.2 and 0.3% W/V. Variables measured were cellulase, xylanase and ligninase activities.Results of the first sudy showed that the isolates obtained consisted of 3 types, those were cellulolyticbacteria (S1, S2, and S3, 3 types of bacteria xylanolytic (X1, X2, and X3 and 3 types of bacteria lignolytic(L1, L2, and L3. Meanwhile, results of the second study showed that isolates of S2, X3, and L1 had thehighest activity, those were 1.894 U/mL, 1.722 U/mL and 0.314 U/mL, respectively. In conclusion, the addition of 1% level of rice straw substrate and 0.3% of nitrogen showed the highest enzyme activity oncellulase, xylanase and ligninase.

  17. Antioxidant Activities of Hydrolysable Tannins and Flavonoid Glycosides Isolated from Eugenia uniflora L.

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    Gilmara A. C. Fortes

    2015-01-01

    Full Text Available Aphytochemical investigation of Eugenia uniflora ’s leaf extract resulted in the isolation of eleven phenolic compounds: 2,3-di-O-galloyl- b - D -glucose (1, 1,2,3,4,6-penta-O-galloyl- b - D -glucose (2, gemin D (3, hippomanin A (4, oenothein B (5, eugeniflorin D 2 (6, camptothin A (7, afzelin (8, quercitrin (9, myricitrin, (10 and desmanthin-1 (11. These compounds were identified by spectroscopic methods including 1D- and 2D-NMR, UV, IR, and TOF/MS. Compounds 1, 2, 3, 4, 7, 8, 9, and 11 were isolated from this species for the first time. Ten isolates were evaluated for antioxidant activity by DPPH free radical and Oxygen Radical Absorbance Capacity (ORAC-Fluorecein assay . Dimeric tannins, oenothein B (5, eugeniflorin D 2 (6, and camptothin A (7 showed a remarkable radical scavenging capacity.

  18. Isolation of thymus gland fractions and the determination of their biological activity

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    MILENA RADETA

    2007-03-01

    Full Text Available A calf thymus extract was prepared and fractionated into lipid and non-lipid fractions. The non-lipid fraction was isolated from the calf thymus extract using the Folch method. The components isolated from the non-lipid fraction were characterized by IR, NMR, biuret and HPLC method. The results of the analyses indicated the presence of peptides. The lipid fraction contained phospholipids, glycolipids and neutral lipids. The biological activity of both the isolated lipid and peptide fractions was determined by the in vivo hemolytic plaques method in Wistar rats with an involuted thymus. The peptide and phospholipid fractions of the thymus extract showed a significant increase of hemolytic plaques. The glycolipid and neutral lipid fraction failed to express a significant immunological response.

  19. Antimicrobial activity of Ulopterol isolated from Toddalia asiatica (L.) Lam.: a traditional medicinal plant.

    Science.gov (United States)

    Karunai Raj, M; Balachandran, C; Duraipandiyan, V; Agastian, P; Ignacimuthu, S

    2012-03-06

    The leaves of Toddalia asiatica (L.) Lam. (Rutaceae) are widely used in folk medicine in India to treat various ailments like cough, malaria, indigestion, influenza lung diseases and rheumatism, fever, stomach ailments, cholera and diarrhea. In our earlier communication we have reported the antimicrobial study on the various extracts of the leaves and the isolation and identification of Flindersine, a quinolone alkaloid as the major active principle. In the present study, we report the antibacterial and antifungal activities of Ulopterol, a coumarin isolated as another major active antimicrobial principle. The leaves were successively extracted with hexane, chloroform, ethyl acetate, methanol and water. The extracts were studied for their antimicrobial activity against selected bacteria and fungi by using disc-diffusion method. The ethyl acetate extract which was found to possess highest antimicrobial activity was subjected to activity guided fractionation by column chromatography over silica gel. This resulted in the isolation of the coumarin, Ulopetrol, an active principle besides Flindersine which was reported by us earlier. The structure of the compound was elucidated using physical and spectroscopic data. Flindersine and Ulopterol were quantified by HPLC. Ulopterol showed activity against the bacteria viz. Staphylococcus epidermidis, Enterobacter aerogenes, Shigella flexneri, Klebsiella pneumoniae (ESBL-3967), Escherichia coli (ESBL-3984) and fungi viz. Aspergillus flavus, Candida krusei and Botrytis cinerea. Quantification by HPLC showed the content of Flindersine and Ulopterol to be 0.361% and 0.266% respectively on dry weight basis of the leaves. Ethyl acetate extract (successive extraction) contained Ulopterol, a coumarin, besides Flindersine, a quinolone alkaloid, as a major active principle in the antimicrobial studies. This is the first report of the antimicrobial activity of Ulopterol and also its first report from the plant. Copyright © 2012

  20. Frequency of endophytic fungi isolated from Dendrobium crumenatum (Pigeon orchid and antimicrobial activity

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    WIBOWO MANGUNWARDOYO

    2012-01-01

    Full Text Available Mangunwardoyo W, Suciatmih, Gandjar I. 2012. Frequency of endophytic fungi isolated from Dendrobium crumenatum (Pigeon orchid and antimicrobial activity. Biodiversitas 13: 34-39. The aims of this research was to isolate and study the frequency of endophytic fungi from roots, bulbous, stems, and leaves of Dendrobium crumenatum Sw. (pigeon orchid collected from Tanah Baru housing area, Bogor Botanical Garden, and Herbarium Bogoriense; and to assess for antimicrobial activity against Candida albicans ATCC 2091, Candida tropicalis LIPIMC 203, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 25923. Twelve species of endophytic fungi were identified from 60 samples obtained from D. crumenatum. Guignardia endophyllicola (anamorph: Phyllosticta capitalensis were the dominant endophytic fungi. Screening of the anti-microorganism activity of the endophytic fungi revealed that Fusarium nivale inhibited C albicans and C. tropicalis. All specimens did not inhibit B. subtilis, E. coli, and S. aureus.

  1. Aporphine alkaloids with antitubercular activity isolated from Ocotea discolor Kunth (Lauraceae

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    Monica Constanza Avila Murillo

    2017-09-01

    Full Text Available Tuberculosis disease causes thousands of deaths worldwide and, currently, the used drugs are either not enough or obsolete for its treatment. Therefore, new compounds that combat this disease are been seek. Thus, the antituberculosis activity of the alkaloids ocoxilonine (1, ocoteine (2, dicentrine (3 and 1,2-methylenedioxy-3,10,11-trimethoxyaporphine (4, isolated from Ocotea discolor wood was evaluated. Their structures were identified by analysis of nuclear magnetic resonance spectroscopic data (NMR 1D – 1H, 13C, 2D – COSY, HSQC and HMBC, mass spectra, and comparison with literature data. All the isolated compounds demonstrated antituberculosis activity, with ocoteine (2 being the most active compound, with a minimum inhibitory concentration value of 140 μM against the virulent strain Mycobacterium tuberculosis H37Rv. All the isolated compounds showed antituberculosis activity, with a variation range in the minimum inhibitory concentration between 140 to 310 μM, being ocoteine (2 the most active compound against the virulent strain Mycobacterium tuberculosis H37Rv.

  2. Antibacterial and antifungal activity of Flindersine isolated from the traditional medicinal plant, Toddalia asiatica (L.) Lam.

    Science.gov (United States)

    Duraipandiyan, V; Ignacimuthu, S

    2009-06-25

    The leaves and root of Toddalia asiatica (L.) Lam. (Rutaceae) are widely used as a folk medicine in India. Hexane, chloroform, ethyl acetate, methanol and water extracts of Toddalia asiatica leaves and isolated compound Flindersine were tested against bacteria and fungi. Antibacterial and antifungal activities were tested against bacteria and fungi using disc-diffusion method and minimum inhibitory concentrations (MICs). The compound was confirmed using X-ray crystallography technique. Antibacterial and antifungal activities were observed in ethyl acetate extract. One active principle Flindersine (2,6-dihydro-2,2-dimethyl-5H-pyrano [3,2-c] quinoline-5-one-9cl) was isolated from the ethyl acetate extract. The MIC values of the compound against bacteria Bacillus subtilis (31.25 microg/ml), Staphylococcus aureus (62.5 microg/ml), Staphylococcus epidermidis (62.5 microg/ml), Enterococcus faecalis (31.25 microg/ml), Pseudomonas aeruginosa (250 microg/ml), Acinetobacter baumannii (125 microg/ml) and fungi Trichophyton rubrum 57 (62.5 microg/ml), Trichophyton mentagrophytes (62.5 microg/ml), Trichophyton simii (62.5 microg/ml), Epidermophyton floccosum (62.5 microg/ml), Magnaporthe grisea (250 microg/ml) and Candida albicans (250 microg/ml) were determined. Ethyl acetate extract showed promising antibacterial and antifungal activity and isolated compound Flindersine showed moderate activity against bacteria and fungi.

  3. Anti-proliferation activity of terpenoids isolated from Euphorbia kansui in human cancer cells and their structure-activity relationship.

    Science.gov (United States)

    Hou, Jin-Jun; Shen, Yao; Yang, Zhou; Fang, Lin; Cai, Lu-Ying; Yao, Shuai; Long, Hua-Li; Wu, Wan-Ying; Guo, De-An

    2017-10-01

    Euphorbia kansui is a commonly used traditional Chinese medicine for the treatment of edema, pleural effusion, and asthma, etc. According to the previous researches, terpenoids in E. kansui possess various biological activities, e.g., anti-virus, anti-allergy, antitumor effects. In this work, twenty five terpenoids were isolated from E. kansui, including thirteen ingenane- and eight jatrophane-type diterpenoids (with two new compounds, kansuinin P and Q) and four triterpenoids. Eighteen of them were analyzed by MTS assay for in vitro anticancer activity in five human cancer cell lines. Structure-activity relationship for 12 ingenane-type diterpenoids in colorectal cancer Colo205 cells were preliminary studied. Significant anti-proliferation activities were observed in human melanoma cells breast cancer MDA-MB-435 cells and Colo205 cells. More than half of the isolated ingenane-type diterpenoids showed inhibitory activities in MDA-MB-435 cells. Eight ingenane- and one jatrophane-type diterpenoids possessed much lower IC 50 values in MDA-MB-435 cells than positive control staurosporine. Preliminary structure-activity relationship analysis showed that substituent on position 20 was important for the activity of ingenane-type diterpenoids in Colo205 cells and substituent on position 3 contributed more significant biological activity of the compounds than that on position 5 in both MDA-MB-435 and Colo205 cells. Copyright © 2017 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  4. Immunomodulatory potencies of isolated compounds from Crataegus azarolus through their antioxidant activities.

    Science.gov (United States)

    Mustapha, Nadia; Mokdad-Bzéouich, Imèn; Sassi, Aicha; Abed, Besma; Ghedira, Kamel; Hennebelle, Thierry; Chekir-Ghedira, Leila

    2016-06-01

    The search of natural immunomodulatory agents has become an area of great interest in order to reduce damage to the human body. In this study, the immunomodulatory potential of Crataegus azarolus and its isolated hyperoside on mouse lymphocytes and macrophages in vitro was assessed. The effect of C. azarolus natural compounds on splenocytes proliferation, natural killer (NK) and cytotoxic T lymphocytes (CTL) activities, and on macrophage-mediated cytotoxicity were assessed by MTT test. Phagocytic activity and inhibition of nitric oxide (NO) release by macrophages were also evaluated. The antioxidant capacity of these products was evaluated by determining their cellular antioxidant activity (CAA) in splenocytes and macrophages. Depending on the concentrations, both ethyl acetate (EA) extract and hyperoside (Hyp) from C. azarolus affect macrophage functions by modulating their lysosomal enzyme activity and nitric oxide release. Whereas, the above-mentioned products significantly promote LPS and lectin-stimulated splenocyte proliferation, implying a potential activation of lymphocytes B and T enhancing humoral and cellular immune responses. Moreover, EA extract and Hyp could enhance the activity of NK and T lymphocytes cells, as well as the macrophages-mediated cytotoxicity against B16F10 cells. The anti-inflammatory activity was concomitant with the cellular antioxidant effect of the tested compounds against macrophages and splenocytes. Collectively, C. azarolus and its isolated hyperoside exhibited an immunomodulatory effect through their antioxidant activity. These findings suggest that C. azarolus should be explored as a novel potential immunomodulatory agent for the treatment of inflammatory diseases.

  5. Endophytic fungi isolated from wheat (Triticum durum Desf.): evaluation of their antimicrobial activity, antioxidant activity and host growth promotion.

    Science.gov (United States)

    Harzallah, Daoud; Sadrati, Nouari; Zerroug, Amina; Dahamna, Saliha; Bouharati, Saddek

    2012-01-01

    The emergence of antibiotic-resistant micro-organisms calls for inventive research and development strategies. The screening for antimicrobial compounds from endophytes is a promising way to meet the increasing threat of drug-resistant strains of human and plant pathogens. Endophytes may be defined as "microbes that colonize living, internal tissues of plants without causing any immediate, overt negative effects". Endophytes are relatively unstudied as potential sources of novel natural products for exploitation in medicine, agriculture, and industry. The purpose of this study was to evaluate several isolated fungi from wheat (Triticum durum Desf.) Mohamed Ben Bachir variety and to select endophytic fungi for further evaluation of its antimicrobial, antioxidant activities and host growth promotion. A total of 20 endophytic fungi have been isolated. Antimicrobial activity was evaluated for crude ethyl acetate extracts using an agar diffusion assay. All extracts showed inhibitory activity on at least one or more pathogenic microorganism, with an average zone of inhibition varied between 7 mm to 25 mm, a large zone of 23 and 25mm against candida albicans and Escherichia coli respectively. The antioxidant capacity of the extracts was evaluated by beta-carotene/linoleic acid assay. Results showed that 70% of these extracts have antioxidant activity, exhibiting 50, 57% to 78, 96% inhibitions. While 30% from them, their inhibitory activity for oxidation of linoleic acid Were less than 50%. Growth promotion ability of these endophytes was tested on seed germination among ten isolates tested, two isolates showed significant growth promotion effects on wheat seeds. From the present work we can conclude that these microorganisms could be promising source of bioactive compounds, growth promotion and warrant further study.

  6. Antifungal Activity of Brazilian Propolis Microparticles against Yeasts Isolated from Vulvovaginal Candidiasis

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    Kelen Fátima Dalben Dota

    2011-01-01

    Full Text Available Propolis, a resinous compound produced by Apis mellifera L. bees, is known to possess a variety of biological activities and is applied in the therapy of various infectious diseases. The aim of this study was to evaluate the in vitro antifungal activity of propolis ethanol extract (PE and propolis microparticles (PMs obtained from a sample of Brazilian propolis against clinical yeast isolates of importance in the vulvovaginal candidiasis (VVC. PE was used to prepare the microparticles. Yeast isolates (n=89, obtained from vaginal exudates of patients with VVC, were exposed to the PE and the PMs. Moreover, the main antifungal drugs used in the treatment of VVC (Fluconazole, Voriconazole, Itraconazole, Ketoconazole, Miconazole and Amphotericin B were also tested. Minimum inhibitory concentration (MIC was determined according to the standard broth microdilution method. Some Candida albicans isolates showed resistance or dose-dependent susceptibility for the azolic drugs and Amphotericin B. Non-C. albicans isolates showed more resistance and dose-dependent susceptibility for the azolic drugs than C. albicans. However, all of them were sensitive or dose-dependent susceptible for Amphotericin B. All yeasts were inhibited by PE and PMs, with small variation, independent of the species of yeast. The overall results provided important information for the potential application of PMs in the therapy of VVC and the possible prevention of the occurrence of new symptomatic episodes.

  7. In vitro antimicrobial activity of linezolid tested against vancomycin-resistant enterococci isolated in Brazilian hospitals

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    Reis Adriana O.

    2001-01-01

    Full Text Available The emergence of vancomycin-resistant enterococci (VRE has been described recently in Brazil. This is in contrast to the USA and Europe, where the VRE appeared in the late 1980s. The progressive increase in VRE isolation poses important problems in the antimicrobial therapy of nosocomial infections. Treatment options and effective antimicrobial agents for VRE are often limited and the possibility of transfer of vancomycin genes to other Gram-positive microorganisms continues. In the search for antimicrobial agents for multiresistant Gram-positive cocci, compounds such as linezolid and quinupristin/dalfopristin have been evaluated. The present study was conducted to evaluate the in vitro activity of the oxazolidinone linezolid and 10 other antimicrobial agents, including quinupristin-dalfopristin, against multiresistant enterococci isolated in Brazilian hospitals. Thirty-three vancomycin resistant isolates (17 Enterococcus faecium and 16 E. faecalis, were analyzed. Strains were isolated from patients at São Paulo Hospital, Oswaldo Cruz Hospital, Hospital do Servidor Público Estadual, Santa Marcelina Hospital, Santa Casa de Misericórdia de São Paulo, and Hospital de Clínicas do Paraná. The samples were tested by a broth microdilution method following the National Committee for Clinical Laboratory Standards (NCCLS recommendations. All isolates were molecular typed using pulsed-field gel electrophoresis (PFGE. Linezolid was the most active compound against these multiresistant enterococci, showing 100% inhibition at the susceptible breakpoints. Quinupristin/dalfopristin and teicoplanin showed poor activity against both species. The molecular typing results suggest that there has been interhospital spread of vancomycin resistant E. faecium and E. faecalis among Brazilian hospitals. The results of this study indicate that linezolid is an appropriate therapeutic option for the treatment of vancomycin-resistant enterococci infections in Brazil.

  8. In Vitro Activity of Antimicrobial Agents against Isolates from Patients with Acute Tonsillopharyngitis in Dakar, Senegal

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    A. Gueye Ndiaye

    2009-01-01

    Full Text Available Streptococcus pyogenes ( S. pyogenes is the most important causative agent of tonsillopharyngitis. Beta-lactam antibiotics, particularly penicillin, are the drug of first choice and macrolides are recommended for patients who are allergic to penicillin. However, other antibiotics are also used for the treatment of streptococcal tonsillopharyngitis. In recent years, the increase in the incidence of respiratory tract pathogens that are resistant to current antibacterial agents highlights the need to monitor the evolution of the resistance of these pathogens to antibiotics. In this study, we assess the susceptibility of 98 isolates of S. pyogenes to 16 antibiotics. The pathogens were recovered from patients with acute tonsillopharyngitis in Dakar, the Senegalese capital city, who were recruited from May 2005 to August 2006. All strains were susceptible to penicillin with low Minimum Inhibitory Concentration (MIC = 0,016 mg/L. Amoxicillin had high activity (100% showing its importance in treatment of streptococcal infections. Cephalosporins had MIC 90 values ranging from 0.016 to 0.094 mg/L. Macrolides have shown high activity. All strains were resistant to tetracyclin. Other molecules such as teicoplanin, levofloxacin and chloramphenicol were also active and would represent alternatives to treatment of tonsillopharyngitis due to this pathogen. These results indicate that no significant resistance to antibiotics was found among patients with tonsillopharyngitis studied in Dakar. Limitations of this study were that the number of isolates tested was small and all isolates were collected from one hospital in Dakar. Hence, results may not be representative of the isolates found, in the wider community or other regions of Senegal. Further studies are needed in other parts of Dakar and other geographic regions of Senegal, in order to better clarify the antibiotic susceptibility profile of S. pyogenes isolates recovered from patients with

  9. Anti-trypanosomal activity of pentacyclic triterpenes isolated from Austroplenckia populnea (Celastraceae).

    Science.gov (United States)

    Duarte, Lucienir Pains; Vieira Filho, Sidney Augusto; Silva, Grácia Divina de Fátima; de Sousa, José Rego; Pinto, Artur da Silveira

    2002-01-01

    Four pentacyclic triterpenes isolated from Austroplenckia populnea and four compounds of known anti T. cruzi or anti-malarial activity were tested. Of those triterpenes tested 20alpha-hydroxy-tingenone showed high activity, epikatonic acid was less active, while populnilic and populninic acids were inactive against the trypanosome of the subgenus Schizotrypanum tested. Benzonidazole, nifurtimox, ketoconazole and primaquine presented a remarkable dose-dependent inhibitory effect reaching practically to a total growth inhibition of the parasite at the end of incubation time. The trypanosome tested appear to be a suitable model for preliminary screen for anti T. (S.) cruzi compounds.

  10. Antimicrobial activity of Algerian honey on subclinical mastitis pathogens isolated from goat's milk

    Directory of Open Access Journals (Sweden)

    A. Bourabah

    2014-04-01

    Full Text Available Aim: The aim of the present study was to determine the susceptibility of subclinical mastitis pathogens isolated from goat's milk and to evaluate the antimicrobial activity of Algerian honey on mastitis causing bacteria. Materials and Methods: The antibacterial activity against the isolated bacteria was evaluated by determining the Minimal Inhibitory Concentration (MIC, using the agar incorporation method. Results: The results showed that both Micrococcus spp. and Klebsiella spp. were susceptible to Streptomycin and tetracycline, while Pseudomonas aeruginosa, E coli, Enterobacter spp., Bacillus spp., and Coagulase Negative Staphyloccoci (CNS were preferentially susceptible to Streptomycin. However, Streptococcus D was the most resistant to the tested antibiotics whereas Staphylococcus aureus was the most susceptible to all the studied antibiotics. As regards to the antimicrobial activity of honey, the measured values were comprised between 11 and 14%. Conclusion: The results reveal that antimicrobial drugs susceptibility tests in goat subclinical mastitis might be necessary before the treatment. Algerian honey exhibited in vitro antimicrobial activity against different isolated bacteria in goat mastitis.

  11. Thymidine uptake, thymidine incorporation, and thymidine kinase activity in marine bacterium isolates

    International Nuclear Information System (INIS)

    Jeffrey, W.H.; Paul, J.H.

    1990-01-01

    One assumption made in bacterial production estimates from [ 3 H]thymidine incorporation is that all heterotrophic bacteria can incorporate exogenous thymidine into DNA. Heterotrophic marine bacterium isolates from Tampa Bay, Fla., Chesapeake Bay, Md., and a coral surface microlayer were examined for thymidine uptake (transport), thymidine incorporation, the presence of thymidine kinase genes, and thymidine kinase enzyme activity. Of the 41 isolates tested, 37 were capable of thymidine incorporation into DNA. The four organisms that could not incorporate thymidine also transported the thymidine poorly and lacked thymidine kinase activity. Attempts to detect thymidine kinase genes in the marine isolates by molecular probing with gene probes made from Escherichia coli and herpes simplex virus thymidine kinase genes proved unsuccessful. To determine if the inability to incorporate thymidine was due to the lack of thymidine kinase, one organism, Vibro sp. strain DI9, was transformed with a plasmid (pGQ3) that contained an E. coli thymidine kinase gene. Although enzyme assays indicated high levels of thymidine kinase activity in transformants, these cells still failed to incorporate exogenous thymidine into DNA or to transport thymidine into cells. These results indicate that the inability of certain marine bacteria to incorporate thymidine may not be solely due to the lack of thymidine kinase activity but may also be due to the absence of thymidine transport systems

  12. In vitro cytotoxic and in silico activity of piperine isolated from Piper nigrum fruits Linn.

    Science.gov (United States)

    Paarakh, Padmaa M; Sreeram, Dileep Chandra; D, Shruthi S; Ganapathy, Sujan P S

    2015-12-01

    Piper nigrum [Piperaceae], commonly known as black pepper is used as medicine fairly throughout the greater part of India and as a spice globally. To isolate piperine and evaluate in vitro cytotoxic [antiproliferative] activity and in silico method. Piperine was isolated from the fruits of P.nigrum. Piperine was characterized by UV,IR, (1)H-NMR, (13)C-NMR and Mass spectrum. Standardization of piperine was done also by HPTLC fingerprinting. In vitro cytotoxic activity was done using HeLa cell lines by MTT assay at different concentrations ranging from 20 to 100 μg/ml in triplicate and in silico docking studies using enzyme EGFR tyrosine kinase. Fingerprinting of isolated piperine were done by HPTLC method. The IC50 value was found to be 61.94 ± 0.054 μg/ml in in vitro cytotoxic activity in HeLa Cell lines. Piperine was subjected to molecular docking studies for the inhibition of the enzyme EGFR tyrosine kinase, which is one of the targets for inhibition of cancer cells. It has shown -7.6 kJ mol(-1) binding and 7.06 kJ mol(-1) docking energy with two hydrogen bonds. piperine has shown to possess in vitro cytotoxic activity and in silico studies.

  13. In vitro antimicrobial activity of five essential oils on multidrug resistant Gram-negative clinical isolates.

    Science.gov (United States)

    Sakkas, Hercules; Gousia, Panagiota; Economou, Vangelis; Sakkas, Vassilios; Petsios, Stefanos; Papadopoulou, Chrissanthy

    2016-01-01

    The emergence of drug-resistant pathogens has drawn attention on medicinal plants for potential antimicrobial properties. The objective of the present study was the investigation of the antimicrobial activity of five plant essential oils on multidrug resistant Gram-negative bacteria. Basil, chamomile blue, origanum, thyme, and tea tree oil were tested against clinical isolates of Acinetobacter baumannii (n = 6), Escherichia coli (n = 4), Klebsiella pneumoniae (n = 7), and Pseudomonas aeruginosa (n = 5) using the broth macrodilution method. The tested essential oils produced variable antibacterial effect, while Chamomile blue oil demonstrated no antibacterial activity. Origanum, Thyme, and Basil oils were ineffective on P. aeruginosa isolates. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration values ranged from 0.12% to 1.50% (v/v) for tea tree oil, 0.25-4% (v/v) for origanum and thyme oil, 0.50% to >4% for basil oil and >4% for chamomile blue oil. Compared to literature data on reference strains, the reported MIC values were different by 2SD, denoting less successful antimicrobial activity against multidrug resistant isolates. The antimicrobial activities of the essential oils are influenced by the strain origin (wild, reference, drug sensitive, or resistant) and it should be taken into consideration whenever investigating the plants' potential for developing new antimicrobials.

  14. Anti-plasmodial activity of some Zulu medicinal plants and of some triterpenes isolated from them.

    Science.gov (United States)

    Simelane, Mthokozisi B C; Shonhai, Addmore; Shode, Francis O; Smith, Peter; Singh, Mogie; Opoku, Andy R

    2013-10-08

    Mimusops caffra E. Mey. ex A.DC and Mimusops obtusifolia Lam (both members of the Sapotaceae family), and Hypoxis colchicifolia Bak (family Hypoxidaceae) are used by traditional healers in Zululand to manage malaria. Anti-plasmodial investigation of the crude extracts and some triterpenes isolated from the plants showed activity against a chloroquine sensitive (CQS) strain of Plasmodium falciparum (D10). Among the crude extracts the leaves of M. caffra exhibited the highest activity, with an IC₅₀ of 2.14 μg/mL. The pentacyclic tritepenoid ursolic acid (1), isolated from the leaves of M. caffra was the most active compound (IC₅₀ 6.8 μg/mL) as compared to taraxerol (2) and sawamilletin (3) isolated from the stem bark of M. obtusifolia (IC₅₀ > 100). Chemical modification of the ursolic acid (1) to 3β-acetylursolic acid (4) greatly enhanced its anti-plasmodial activity. Compound 4 reduced parasitaemia against Plasmodium berghei by 94.01% in in vivo studies in mice. The cytotoxicity of 3β-acetylursolic acid (IC₅₀) to two human cell lines (HEK293 and HepG2) was 366.00 μg/mL and 566.09 μg/mL, respectively. The results validate the use of these plants in folk medicine.

  15. Isolation of compounds from Sceletium tortuosum and the detection of antimalarial activity of the isolates and extracts

    CSIR Research Space (South Africa)

    Setshedi, I

    2010-09-01

    Full Text Available carbons signals in the aromatic region between 108.9 ppm and 148.8 ppm. Figure 3.1: 600 MHz 1H nMr spectrum and structure of mesembrine A second isolated compound was suspected to be pinitol. this was based on the characteristics presented on tlc... and for social and spiritual purposes by San hunter-gatherers and Khoi pastoralists (Gericke and Viljoen, 2008). objectIVeS the study focused on the phytochemical isolation of compounds and biological screening of isolated constituents together with crude...

  16. Activity-Guided Isolation of Bioactive Constituents with Antinociceptive Activity from Muntingia calabura L. Leaves Using the Formalin Test

    Directory of Open Access Journals (Sweden)

    Mohd. Izwan Mohamad Yusof

    2013-01-01

    Full Text Available The present study was conducted to determine the antinociceptive potential of methanol extract of Muntingia calabura L. (MEMC and to isolate and identify the bioactive compound(s responsible for the observed antinociceptive activity. The MEMC and its partitions (petroleum ether (PEP, ethyl acetate (EAP, and aqueous (AQP partitions, in the dose range of 100, 500, and 1000 mg/kg, were tested using the formalin-induced nociceptive test. The PEP, which exerted the most effective activity in the respective early and late phase, was further subjected to the fractionation procedures and yielded seven fractions (labelled A to G. These fractions were tested, at the dose of 300 mg/kg, together with distilled water or 10% DMSO (negative controls; morphine and aspirin (positive controls for potential antinociceptive activity. Of all fractions, Fraction D showed the most significant antinociceptive activity, which is considered as equieffective to morphine or aspirin in the early or late phase, respectively. Further isolation and identification processes on fraction D led to the identification of three known and one new compounds, namely, 5-hydroxy-3,7,8-trimethoxyflavone (1, 3,7-dimethoxy-5-hydroyflavone (2, 2′,4′-dihydroxy-3′-methoxychalcone (3, and calaburone (4. At the dose of 50 mg/kg, compound 3 exhibited the highest percentage of antinociceptive activity in both phases of the formalin test. In conclusion, the antinociceptive activity of MEMC involved, partly, the synergistic activation of the flavonoid types of compounds.

  17. Influence of Rack Slope and Approaching Conditions in Bottom Intake Systems

    Directory of Open Access Journals (Sweden)

    Luis G. Castillo

    2017-01-01

    Full Text Available The study analyzes the flow over bottom racks made of longitudinal T-shaped bars. A clear water flow is considered in a laboratory flume. Free surface profiles, wetted rack lengths, and discharge coefficients are measured, changing parameters such as longitudinal slope, void ratio, and approaching flow. The present work complements existing experimental studies, considering the influence of the approaching flow conditions. The velocity field measured with Particle Image Velocimetry (PIV technique and the pressure field with Pitot tubes are quantified. Numerical simulations (CFD are used to complement laboratory data. The energy head along the rack is calculated and compared with the hypothesis of horizontal energy level with minimum energy at the beginning of the rack. A discharge coefficient adjustment that considers the slope, the void ratio, and the position along the rack is proposed and presented with the results of other works. Theoretical proposals to calculate the pressure field along the flow are compared with measurements in the laboratory. The relation between the static pressure head in the space of bars and the discharge coefficient is used as an alternative method to define the discharge.

  18. Implications of natural occlusion of ventilated racks on ammonia and sanitation practices.

    Science.gov (United States)

    Creamer, Michelle A; Petty, Joann; Martin, Tara; Bergdall, Valerie; Hickman-Davis, Judy M

    2014-03-01

    Examination of ventilated rat racks prior to semiannual sanitation revealed silicone nozzles and ventilation ports that were partially or completely occluded with granular debris. We subsequently sought to document performance standards for rack sanitation and investigate the effect of ventilation port occlusion on rack function and animal husbandry practices. We hypothesized that individually ventilated cages with occluded airflow would require more frequent cage changes, comparable to those for static cages (that is, every 3 to 4 d). Sprague-Dawley rats were housed under one of 4 conditions: no airflow occlusion, occluded air-supply inlet, occluded air-exhaust outlet, and occlusion of both inlet and outlet. Cages were changed when daily ammonia concentration exceeded 20 ppm or after 14 d had elapsed. Most cages with unoccluded or partial airflow occlusion remained below the 20 ppm limit until day 12 or 13. Cages with occlusion of both inlet and outlet exceeded 20 ppm ammonia by as early as day 5. Airflow was significantly lower in cages with occlusion of both inlet and outlet airflow. Weekly inspection revealed that occlusion of ventilation ports was detectable by 3 mo after semiannual sanitation. This study demonstrates that silicone nozzles should be removed prior to rack sanitation to improve the effectiveness of cleaning ventilation ports and nozzles. While the rack is in use, silicone nozzles and ventilation ports should be inspected regularly to identify occlusion that is likely to diminish environmental quality in the cage. Intracage ammonia levels are significantly higher when both inlet and outlet airflow are occluded.

  19. Evaluation of food storage racks available on the Polish market in the hygienic context

    Science.gov (United States)

    Grzesińska, Wiesława; Tomaszewska, Marzena; Bilska, Beata; Trafiałek, Joanna; Dziadek, Michał

    Providing safe food products to the consumer depends on the material and technology used and adherence to hygienic practices, throughout the production process. The degree of microbial contamination of a surface is an important indicator of equipment cleanliness and effectiveness of cleaning and disinfection. Used material, construction solutions and quality of the applied devices also have an effect on hygienic status. The objective of the present study was to evaluate the influence of the design and construction material of selected food storage racks, available on the Polish market, on their hygienic status. The study was based on determination of the capability of microbial growth on the surface of the racks and the effectiveness of their cleaning. Microbiological cleanliness on the surface of the racks was monitored by the contact plates which are able to estimate the total number of icroorganisms. Examination of effectiveness of cleaning was conducted by the use of ATP bioluminescence method. This experiment has proven a significant influence of adopted construction solutions on the hygienic status of the examined racks. Presence of antibacterial layer and a choice of the appropriate construction material characterized by a low surface roughness impedes the microbial growth and increases the effectiveness of cleaning. Design solutions have significant impact on the hygienic status of shelves. Selection of a suitable material for the construction of racks can greatly reduce the possibility of the development of microorganism, despite the low efficiency of the cleaning. The application of antimicrobial coatings inhibits microbial growth.

  20. SCORHE: a novel and practical approach to video monitoring of laboratory mice housed in vivarium cage racks.

    Science.gov (United States)

    Salem, Ghadi H; Dennis, John U; Krynitsky, Jonathan; Garmendia-Cedillos, Marcial; Swaroop, Kanchan; Malley, James D; Pajevic, Sinisa; Abuhatzira, Liron; Bustin, Michael; Gillet, Jean-Pierre; Gottesman, Michael M; Mitchell, James B; Pohida, Thomas J

    2015-03-01

    The System for Continuous Observation of Rodents in Home-cage Environment (SCORHE) was developed to demonstrate the viability of compact and scalable designs for quantifying activity levels and behavior patterns for mice housed within a commercial ventilated cage rack. The SCORHE in-rack design provides day- and night-time monitoring with the consistency and convenience of the home-cage environment. The dual-video camera custom hardware design makes efficient use of space, does not require home-cage modification, and is animal-facility user-friendly. Given the system's low cost and suitability for use in existing vivariums without modification to the animal husbandry procedures or housing setup, SCORHE opens up the potential for the wider use of automated video monitoring in animal facilities. SCORHE's potential uses include day-to-day health monitoring, as well as advanced behavioral screening and ethology experiments, ranging from the assessment of the short- and long-term effects of experimental cancer treatments to the evaluation of mouse models. When used for phenotyping and animal model studies, SCORHE aims to eliminate the concerns often associated with many mouse-monitoring methods, such as circadian rhythm disruption, acclimation periods, lack of night-time measurements, and short monitoring periods. Custom software integrates two video streams to extract several mouse activity and behavior measures. Studies comparing the activity levels of ABCB5 knockout and HMGN1 overexpresser mice with their respective C57BL parental strains demonstrate SCORHE's efficacy in characterizing the activity profiles for singly- and doubly-housed mice. Another study was conducted to demonstrate the ability of SCORHE to detect a change in activity resulting from administering a sedative.

  1. Phylogenetic diversity and biological activity of culturable Actinobacteria isolated from freshwater fish gut microbiota.

    Science.gov (United States)

    Jami, Mansooreh; Ghanbari, Mahdi; Kneifel, Wolfgang; Domig, Konrad J

    2015-06-01

    The diversity of Actinobacteria isolated from the gut microbiota of two freshwater fish species namely Schizothorax zarudnyi and Schizocypris altidorsalis was investigated employing classical cultivation techniques, repetitive sequence-based PCR (rep-PCR), partial and full 16S rDNA sequencing followed by phylogenetic analysis. A total of 277 isolates were cultured by applying three different agar media. Based on rep-PCR profile analysis a subset of 33 strains was selected for further phylogenetic investigations, antimicrobial activity testing and diversity analysis of secondary-metabolite biosynthetic genes. The identification based on 16S rRNA gene sequencing revealed that the isolates belong to eight genera distributed among six families. At the family level, 72% of the 277 isolates belong to the family Streptomycetaceae. Among the non-streptomycetes group, the most dominant group could be allocated to the family of Pseudonocardiaceae followed by the members of Micromonosporaceae. Phylogenetic analysis clearly showed that many of the isolates in the genera Streptomyces, Saccharomonospora, Micromonospora, Nocardiopsis, Arthrobacter, Kocuria, Microbacterium and Agromyces formed a single and distinct cluster with the type strains. Notably, there is no report so far about the occurrence of these Actinobacteria in the microbiota of freshwater fish. Of the 33 isolates, all the strains exhibited antibacterial activity against a set of tested human and fish pathogenic bacteria. Then, to study their associated potential capacity to synthesize diverse bioactive natural products, diversity of genes associated with secondary-metabolite biosynthesis including PKS I, PKS II, NRPS, the enzyme PhzE of the phenazine pathways, the enzyme dTGD of 6-deoxyhexoses glycosylation pathway, the enzyme Halo of halogenation pathway and the enzyme CYP in polyene polyketide biosynthesis were investigated among the isolates. All the strains possess at least two types of the investigated

  2. Antimicrobial activity of extracellular metabolites from antagonistic bacteria isolated from potato (Solanum phureja crops

    Directory of Open Access Journals (Sweden)

    Sinar David Granada García

    2014-09-01

    Full Text Available Microorganisms for biological control are capable of producing active compounds that inhibit the development of phytopathogens, constituting a promising tool toob tain active principles that could replace synthetic pesticides. This study evaluatedtheability of severalpotentialbiocontrol microorganismsto produce active extracellular metabolites. In vitro antagonistic capability of 50 bacterial isolates from rhizospheric soils of "criolla" potato (Solanum phureja was tested through dual culture in this plant with different plant pathogenic fungi and bacteria. Isolates that showed significantly higher antagonistic activity were fermented in liquid media and crude extracts from the supernatants had their biological activities assessed by optical density techniques. Inhibitory effecton tested pathogens was observed for concentrations between 0.5% and 1% of crude extracts. There was a correlation between the antimicrobial activity of extracts and the use of nutrient-rich media in bacteria fermentation. Using a bioguided method, a peptidic compound, active against Fusarium oxysporum, was obtained from the 7ANT04 strain (Pyrobaculum sp.. Analysis by nuclear magnetic resonance and liquid chromatography coupled to mass detector evidenced an 11-amino acid compound. Bioinformatic software using raw mass data confirmed the presence of a cyclic peptide conformed by 11 mostly non-standard amino acids.

  3. Biological activities from extracts of endophytic fungi isolated from Viguiera arenaria and Tithonia diversifolia.

    Science.gov (United States)

    Guimarães, Denise O; Borges, Warley S; Kawano, Cristina Y; Ribeiro, Patrícia H; Goldman, Gustavo H; Nomizo, Auro; Thiemann, Otávio H; Oliva, Glaucius; Lopes, Norberto P; Pupo, Mônica T

    2008-01-01

    A total of 39 endophytic fungi have been isolated from Viguiera arenaria and Tithonia diversifolia, both collected in São Paulo State, Brazil. The isolates were identified based on their ribosomal DNA sequences. The ethyl acetate (EtOAc) extracts of all endophytic fungi were evaluated for their antimicrobial, antiparasitic and antitumoral activity. Antimicrobial screening was conducted using an agar diffusion assay against three pathogenic microorganisms: Staphylococcus aureus, Escherichia coli and Candida albicans. Antiparasitic activity was determined by enzymatic inhibition of gGAPDH of Trypanosoma cruzi and adenine phosphorybosiltransferase (APRT) of Leishmania tarentolae. Antitumoral activity was tested against human T leukemia cells by the Mosmann colorimetric method. All extracts showed activity in at least one assay: 79.5% of the extracts were cytotoxic against leukemia cells, 5.1% of the extracts were active against S. aureus, 25.6% against E. coli and 64.1% against Candida albicans. Only one extract showed promising results in the inhibition of parasitic enzymes gGAPDH (95.0%) and three were found to inhibit APRT activity. The cytotoxic extract produced by the strain VA1 (Glomerella cingulata) was fractionated and yielded nectriapyrone and tyrosol. Nectriapyrone showed relevant cytotoxic activity against both human T leukemia and melanoma tumor cell lines.

  4. Antioxidant activities of isolated compounds from stems of Mimosa invisa Mart. ex Colla

    Energy Technology Data Exchange (ETDEWEB)

    Aguiar, Rosane M. [Departamento de Quimica e Exatas, Universidade Estadual do Sudoeste da Bahia, Jequie, BA (Brazil); Alves, Clayton Q.; David, Jorge M.; Rezende, Larissa C. de; Lima, Luciano S., E-mail: jmdavid@ufba.br [Instituto de Quimica, Universidade Federal da Bahia, Salvador, BA (Brazil); David, Juceni P. [Faculdade de Farmacia, Universidade Federal da Bahia, Salvador, BA (Brazil); Queiroz, Luciano P. de [Departamento de Ciencias Biologicas, Universidade Estadual de Feira de Santana, BA (Brazil)

    2012-07-01

    This work describes the phytochemical study of stems of Mimosa invisa (Mimosaceae) and the evaluation of the antioxidant potential of isolated compounds. Chromatographic techniques were employed to isolate salicifoliol, pinoresinol, quercetin, quercetin-3-Orhamnopyranosyl, quercetin-3-O-arabinofuranosyl lupeol, -amyrin, sitosterol, p-hydroxy coumaric acid, 4-hydroxy-3-methoxy benzaldehyde (vanillin), 4-hydroxy-3,5-dimethoxy benzaldehyde, 4-hydroxy-3-methoxy benzoic acid and 4',6,7- trimethoxy flavonol. The latter had been previously described but the spectrometric data shown indicated the structure required review. The antioxidant activity of the compounds was evaluated by the DPPH test and capability of NBT reduction by superoxide radicals. Quercetin glycosides showed lower antioxidant potential than quercetin and, salicifoliol was found to be more active than pinoresinol. (author)

  5. Phytochemical Profile and Qualification of Biological Activity of an Isolated Fraction of Bellis perennis

    Directory of Open Access Journals (Sweden)

    Thiago H Costa Marques

    2013-01-01

    Full Text Available This study describes the isolation and identification of apigenin-7-O-ghicopyranoside, a flavonoid isolated from the flowers of Bellis perennis L., Asteraceae, an species with a broad spectrum of biological activities. The in vitro antioxidant activity and the inhibition of the enzyme acetylcholinesterase were evaluated. The flavonoid showed strong in vitro antioxidant potential, because of the capacity of removal of hydroxyl radicals and nitric oxide, and also prevented the formation of thiobarbituric acid-reactive substances. These parameters were inhibited at the highest concentration of ApG at rates of 77.7%, 72% and 73.4%, respectively, in addition to inhibiting acetylcholinesterase, suggesting potential use in the treatment of neurodegenerative diseases.

  6. A tubular dielectric elastomer actuator: Fabrication, characterization and active vibration isolation

    DEFF Research Database (Denmark)

    Sarban, R.; Jones, R. W.; Mace, B. R.

    2011-01-01

    This contribution reviews the fabrication, characterization and active vibration isolation performance of a core-free rolled tubular dielectric elastomer (DE) actuator, which has been designed and developed by Danfoss PolyPower A/S. PolyPower DE material, PolyPower (TM), is produced in thin sheets...... of 80 mu m thickness with corrugated metallic electrodes on both sides. Tubular actuators are manufactured by rolling the DE sheets in a cylindrical shape. The electromechanical characteristics of such actuators are modeled based on equilibrium pressure equation. The model is validated with experimental...... the dominant dynamic characteristics of the core-free tubular actuator. It has been observed that all actuators have similar dynamic characteristics in a frequency range up to 1 kHz. A tubular actuator is then used to provide active vibration isolation (AVI) of a 250 g mass subject to shaker generated 'ground...

  7. Isolation and antitrichomonal activity of the chemical constituents of the leaves of Maytenus phyllanthoides Benth. (Celastraceae)

    International Nuclear Information System (INIS)

    Moo-Puc, Juan Alberto; Martin-Quintal, Zhelmy; Miron-Lopez, Gumersindo; Mena-Rejon, Gonzalo J.; Moo-Puc, Rosa Esther; Quijano, Leovigildo

    2014-01-01

    Cyclolignan (+)-lyoniresinol (1), veratric acid (2), vanillic acid (3), lupeol, oleanolic acid, 3β-hydroxy-urs-11-en-28,13β-lactone (4), the mixture of α- and β-amyrin, trans-polyisoprene, and β-sitosterol were isolated from the leaves of Maytenus phyllanthoides. The structures of the isolated compounds were established based on spectroscopic data, mainly 1 H and 13 C nuclear magnetic resonance (NMR). Compound 1, its acetate analog 1a, and compounds 2, 3, and 4 were tested against Trichomonas vaginalis. (+)-Lyoniresinol showed activity corresponding to IC 50 17.57 μM. This is the first report on the occurrence of 3β-hydroxy-urs-11-en-28,13β-lactone (4) in the Celastraceous family and lyoniresinol in the Maytenus genus, and on the antitrichomonal activity of lyoniresinol. (author)

  8. Isolation and antitrichomonal activity of the chemical constituents of the leaves of Maytenus phyllanthoides Benth. (Celastraceae)

    Energy Technology Data Exchange (ETDEWEB)

    Moo-Puc, Juan Alberto; Martin-Quintal, Zhelmy; Miron-Lopez, Gumersindo; Mena-Rejon, Gonzalo J., E-mail: mrejon@uady.mx [Laboratorio de Quimica Farmaceutica. Facultad de Quimica. Universidad Autonoma de Yucatan (Mexico); Moo-Puc, Rosa Esther [Unidad de Investigacion Medica Yucatan, Instituto Mexicano del Seguro Social, Yucatan (Mexico); Quijano, Leovigildo [Instituto de Quimica, Universidad Nacional Autonoma de Mexico, Coyoacan (Mexico)

    2014-07-01

    Cyclolignan (+)-lyoniresinol (1), veratric acid (2), vanillic acid (3), lupeol, oleanolic acid, 3β-hydroxy-urs-11-en-28,13β-lactone (4), the mixture of α- and β-amyrin, trans-polyisoprene, and β-sitosterol were isolated from the leaves of Maytenus phyllanthoides. The structures of the isolated compounds were established based on spectroscopic data, mainly {sup 1}H and {sup 13}C nuclear magnetic resonance (NMR). Compound 1, its acetate analog 1a, and compounds 2, 3, and 4 were tested against Trichomonas vaginalis. (+)-Lyoniresinol showed activity corresponding to IC{sub 50} 17.57 μM. This is the first report on the occurrence of 3β-hydroxy-urs-11-en-28,13β-lactone (4) in the Celastraceous family and lyoniresinol in the Maytenus genus, and on the antitrichomonal activity of lyoniresinol. (author)

  9. Isolation and antitrichomonal activity of the chemical constituents of the leaves of Maytenus phyllanthoides Benth. (Celastraceae

    Directory of Open Access Journals (Sweden)

    Juan Alberto Moo-Puc

    2014-01-01

    Full Text Available Cyclolignan (+-lyoniresinol (1, veratric acid (2, vanillic acid (3, lupeol, oleanolic acid, 3β-hydroxy-urs-11-en-28,13β-lactone (4, the mixture of α- and β-amyrin, trans-polyisoprene, and β-sitosterol were isolated from the leaves of Maytenus phyllanthoides. The structures of the isolated compounds were established based on spectroscopic data, mainly ¹H and 13C nuclear magnetic resonance (NMR. Compound 1, its acetate analog 1a, and compounds 2, 3, and 4 were tested against Trichomonas vaginalis. (+-Lyoniresinol showed activity corresponding to IC50 17.57 µM. This is the first report on the occurrence of 3β-hydroxy-urs-11-en-28,13β-lactone (4 in the Celastraceous family and lyoniresinol in the Maytenus genus, and on the antitrichomonal activity of lyoniresinol.

  10. Isolation of Vaginal Lactobacilli and Characterization of Anti-Candida Activity.

    Directory of Open Access Journals (Sweden)

    Carola Parolin

    Full Text Available Healthy vaginal microbiota is dominated by Lactobacillus spp., which form a critical line of defence against pathogens, including Candida spp. The present study aims to identify vaginal lactobacilli exerting in vitro activity against Candida spp. and to characterize their antifungal mechanisms of action. Lactobacillus strains were isolated from vaginal swabs of healthy premenopausal women. The isolates were taxonomically identified to species level (L. crispatus B1-BC8, L. gasseri BC9-BC14 and L. vaginalis BC15-BC17 by sequencing the 16S rRNA genes. All strains produced hydrogen peroxide and lactate. Fungistatic and fungicidal activities against C. albicans, C. glabrata, C. krusei, C. tropicalis, C. parapsilosis and C. lusitaniae were evaluated by broth micro-dilution method. The broadest spectrum of activity was observed for L. crispatus BC1, BC4, BC5 and L. vaginalis BC15, demonstrating fungicidal activity against all isolates of C. albicans and C. lusitaniae. Metabolic profiles of lactobacilli supernatants were studied by 1H-NMR analysis. Metabolome was found to be correlated with both taxonomy and activity score. Exclusion, competition and displacement experiments were carried out to investigate the interference exerted by lactobacilli toward the yeast adhesion to HeLa cells. Most Lactobacillus strains significantly reduced C. albicans adhesion through all mechanisms. In particular, L. crispatus BC2, L. gasseri BC10 and L. gasseri BC11 appeared to be the most active strains in reducing pathogen adhesion, as their effects were mediated by both cells and supernatants. Inhibition of histone deacetylases was hypothesised to support the antifungal activity of vaginal lactobacilli. Our results are prerequisites for the development of new therapeutic agents based on probiotics for prophylaxis and adjuvant therapy of Candida infection.

  11. Anti-BACE1 and Antimicrobial Activities of Steroidal Compounds Isolated from Marine Urechis unicinctus

    OpenAIRE

    Yong-Zhe Zhu; Jing-Wen Liu; Xue Wang; In-Hong Jeong; Young-Joon Ahn; Chuan-Jie Zhang

    2018-01-01

    The human β-site amyloid cleaving enzyme (BACE1) has been considered as an effective drug target for treatment of Alzheimer’s disease (AD). In this study, Urechis unicinctus (U. unicinctus), which is a Far East specialty food known as innkeeper worm, ethanol extract was studied by bioassay-directed fractionation and isolation to examine its potential β-site amyloid cleaving enzyme inhibitory and antimicrobial activity. The following compounds were characterized: hecogenin, cholest-4-en-3-one,...

  12. Probiotic Potential of Lactobacillus Strains with Antifungal Activity Isolated from Animal Manure

    OpenAIRE

    Ilavenil, Soundharrajan; Park, Hyung Soo; Vijayakumar, Mayakrishnan; Valan Arasu, Mariadhas; Kim, Da Hye; Ravikumar, Sivanesan; Choi, Ki Choon

    2015-01-01

    The aim of the study was to isolate and characterize the lactic acid bacteria (LAB) from animal manure. Among the thirty LAB strains, four strains, namely, KCC-25, KCC-26, KCC-27, and KCC-28, showed good cell growth and antifungal activity and were selected for further characterization. Biochemical and physiology properties of strains confirmed that the strains are related to the Lactobacillus sp.; further, the 16S rRNA sequencing confirmed 99.99% sequence similarity towards Lactobacillus pla...

  13. Unraveling the Lipolytic Activity of Thermophilic Bacteria Isolated from a Volcanic Environment

    OpenAIRE

    Stathopoulou, Panagiota M.; Savvides, Alexander L.; Karagouni, Amalia D.; Hatzinikolaou, Dimitris G.

    2013-01-01

    In a bioprospecting effort towards novel thermostable lipases, we assessed the lipolytic profile of 101 bacterial strains isolated from the volcanic area of Santorini, Aegean Sea, Greece. Screening of lipase activity was performed both in agar plates and liquid cultures using olive oil as carbon source. Significant differences were observed between the two screening methods with no clear correlation between them. While the percentage of lipase producing strains identified in agar plates was o...

  14. Isolation, Purification, and Characterization of Five Active Diketopiperazine Derivatives from Endophytic Streptomyces SUK 25 with Antimicrobial and Cytotoxic Activities.

    Science.gov (United States)

    Alshaibani, Muhanna; Zin, Noraziah; Jalil, Juriyati; Sidik, Nik; Ahmad, Siti Junaidah; Kamal, Nurkhalida; Edrada-Ebel, Ruangelie

    2017-07-28

    In our search for new sources of bioactive secondary metabolites from Streptomyces sp., the ethyl acetate extracts from endophytic Streptomyces SUK 25 afforded five active diketopiperazine (DKP) compounds. The aim of this study was to characterize the bioactive compounds isolated from endophytic Streptomyces SUK 25 and evaluate their bioactivity against multiple drug resistance (MDR) bacteria such as Enterococcus raffinosus, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter spp., and their cytotoxic activities against the human hepatoma (HepaRG) cell line. The production of secondary metabolites by this strain was optimized through Thornton's medium. Isolation, purification, and identification of the bioactive compounds were carried out using high-performance liquid chromatography, high-resolution mass liquid chromatography-mass spectrometry, Fourier transform infrared spectroscopy, and nuclear magnetic resonance, and cryopreserved HepaRG cells were selected to test the cytotoxicity. The results showed that endophytic Streptomyces SUK 25 produces four active DKP compounds and an acetamide derivative, which were elucidated as cyclo -( L -Val- L -Pro), cyclo -( L -Leu- L -Pro), cyclo -( L -Phe- L -Pro), cyclo -( L -Val- L -Phe), and N -(7-hydroxy-6-methyl-octyl)-acetamide. These active compounds exhibited activity against methicillin-resistant S. aureus ATCC 43300 and Enterococcus raffinosus , with low toxicity against human hepatoma HepaRG cells. Endophytic Streptomyces SUK 25 has the ability to produce DKP derivatives biologically active against some MDR bacteria with relatively low toxicity against HepaRG cells line.

  15. Potentiating antibiotics in drug-resistant clinical isolates via stimuli-activated superoxide generation.

    Science.gov (United States)

    Courtney, Colleen M; Goodman, Samuel M; Nagy, Toni A; Levy, Max; Bhusal, Pallavi; Madinger, Nancy E; Detweiler, Corrella S; Nagpal, Prashant; Chatterjee, Anushree

    2017-10-01

    The rise of multidrug-resistant (MDR) bacteria is a growing concern to global health and is exacerbated by the lack of new antibiotics. To treat already pervasive MDR infections, new classes of antibiotics or antibiotic adjuvants are needed. Reactive oxygen species (ROS) have been shown to play a role during antibacterial action; however, it is not yet understood whether ROS contribute directly to or are an outcome of bacterial lethality caused by antibiotics. We show that a light-activated nanoparticle, designed to produce tunable flux of specific ROS, superoxide, potentiates the activity of antibiotics in clinical MDR isolates of Escherichia coli , Salmonella enterica , and Klebsiella pneumoniae . Despite the high degree of antibiotic resistance in these isolates, we observed a synergistic interaction between both bactericidal and bacteriostatic antibiotics with varied mechanisms of action and our superoxide-producing nanoparticles in more than 75% of combinations. As a result of this potentiation, the effective antibiotic concentration of the clinical isolates was reduced up to 1000-fold below their respective sensitive/resistant breakpoint. Further, superoxide-generating nanoparticles in combination with ciprofloxacin reduced bacterial load in epithelial cells infected with S. enterica serovar Typhimurium and increased Caenorhabditis elegans survival upon infection with S. enterica serovar Enteriditis, compared to antibiotic alone. This demonstration highlights the ability to engineer superoxide generation to potentiate antibiotic activity and combat highly drug-resistant bacterial pathogens.

  16. Rhodotorula mucilaginosa, a quorum quenching yeast exhibiting lactonase activity isolated from a tropical shoreline.

    Science.gov (United States)

    Ghani, Norshazliza Ab; Sulaiman, Joanita; Ismail, Zahidah; Chan, Xin-Yue; Yin, Wai-Fong; Chan, Kok-Gan

    2014-04-09

    Two microbial isolates from a Malaysian shoreline were found to be capable of degrading N-acylhomoserine lactones. Both Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry and 18S rDNA phylogenetic analyses confirmed that these isolates are Rhodotorula mucilaginosa. Quorum quenching activities were detected by a series of bioassays and rapid resolution liquid chromatography analysis. The isolates were able to degrade various quorum sensing molecules namely N-hexanoyl-L-homoserine lactone (C6-HSL), N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-L-homoserine lactone (3-hydroxy-C6-HSL). Using a relactonisation assay to verify the quorum quenching mechanism, it is confirmed that Rh. mucilaginosa degrades the quorum sensing molecules via lactonase activity. To the best of our knowledge, this is the first documentation of the fact that Rh. mucilaginosa has activity against a broad range of AHLs namely C6-HSL, 3-oxo-C6-HSL and 3-hydroxy-C6-HSL.

  17. Characterization of Bacillus thuringiensis soil isolates from Cuba, with insecticidal activity against mosquitoes

    Directory of Open Access Journals (Sweden)

    Aileen González

    2011-09-01

    Full Text Available Chemical insecticides may be toxic and cause environmental degradation. Consequently, biological control for insects represents an alternative with low ecological impact. In this work, three soil isolates (A21, A51 and C17 from different regions of the Cuban archipelago were identified, characterized and evaluated against Aedes aegypti and Culex quinquefasciatus. The new isolates were compared with reference IPS82 strain and two strains isolated from biolarvicides Bactivec and Bactoculicida, respectively. The differentiation was done by morphological, biochemical, bioassays activity and molecular methods (SDS-PAGE, plasmid profile and random amplified polymorphic analysis. All isolates were identified as Bacillus thuringiensis. The A21, A51 and C17 isolates showed higher larvicide activity than Bactivec’s isolated reference strain, against both A. aegypti and C. quinquefasciatus. A21 isolate had a protein profile similar to IPS82 and Bactivec strain. A51 and C17 isolates produced a characteristic proteins pattern. A21 and A51 isolates had plasmid patterns similar to IPS82 standard strain, while C17 isolate had different both plasmid profile and protein bands. All the studied isolates showed a diverse RAPD patterns and were different from the strains previously used in biological control in Cuba. Rev. Biol. Trop. 59 (3: 1007-1016. Epub 2011 September 01.El uso prolongado de insecticidas ha conducido al desarrollo de resistencia en diferentes especies de mosquitos y al incremento de la degradación del ambiente. El control biológico de insectos ha devenido como una alternativa útil y de bajo impacto ambiental. En nuestro estudio fueron identificados, caracterizados tres aislamientos de suelos procedentes de diferentes regiones del archipiélago cubano y comparados con cepas de referencia: aisladas de los biolarvicidas Bactivec y Bactoculicida, además de IPS82. La diferenciación de los mismos se llevó a cabo mediante métodos morfol

  18. Isolation and partial characterization of soils actinomycetes with antimicrobial activity against multidrug-resistant bacteria

    Directory of Open Access Journals (Sweden)

    Romina Belén Parada

    2017-07-01

    Full Text Available Two hundred and thirty four actinobacteria strains were isolated from Argentinian and Peruvian soil in order to evaluate the antimicrobial activity against multidrug resistant bacteria On the basis of their antagonist activity against methicillin-resistant Staphylococcus aureus (MRSA and two vancomycin-resistant Enterococcus (EVR-Van A and  EVR Van B,13 strains were selected. The presence of NRPS, PKS-I and PKS-II genes were also investigated by PCR techniques. Among the 13 selected actinobacteria, strain AC69C displayed the higher activity in diffusion tests in solid medium and was further evaluated for the production of antagonist metabolites in liquid media. The best results were obtained using fermentation broth with carbohydrates, when starch and glucose were used in combination. Antimicrobial activities of 640 arbitrary units (AU, 320 AU, 320 AU and 80 AU were obtained against EVR-Van A, EVR-Van B, Listeria monocytogenes ATCC7644 and MRSA, respectively. PCR amplification of 16S rRNA gene and subsequent phylogenetic analysis of AC69C strain displayed a 100 % homology with Streptomyces antibioticus NRRL B-1701. It was not possible to establish a correlation between the amplified genes and antimicrobial activity of the 13 selected strains. The results of this work show the wide distribution of actinobacteria in soil and the importance of the isolation of strain to screen novel active metabolites against multidrug resistant bacteria of clinical origin.

  19. Activity-Guided Isolation of Antioxidant Compounds from Andrographis stenophylla Leaf

    Directory of Open Access Journals (Sweden)

    Neelaveni Thangavel

    2010-01-01

    Full Text Available The antioxidant potency of various extracts of Andrographis stenophylla leaf was evaluated in vitro using ferric thiocyanate method. Reductive ability and free radical scavenging activity of the extracts were also investigated. Amounts of phenolic compounds in each of the extracts were determined using Folin-Ciocalteau reagent and compared to observe the correlation between antioxidant activities and total phenolic content. Methanol extract exhibited maximum antioxidant activity and was found to contain 2% of total phenolic compounds. Methanol extract was subjected to column chromatographic separation over silica gel G using ethyl acetate: formic acid: acetic acid: water. Fractions thus obtained were screened for their antioxidant activity. Among the eleven fractions screened, fraction C was more active than the standard butylated hydroxyanisole. Fraction C on further fractionation with n-butanol: acetic acid: water afforded two flavanoids namely acacetine and isosakuranetine. Fraction A was also shown to possess good antioxidant activity which was developed using TLC and indicated the presence of a terpenoid, Andrographolide. The structures of the isolated compounds were confirmed by UV, IR, MS, 1H and 13C NMR spectral data. This is the first report wherein Andrographolide, Acacetine and Isosakuranetine are isolated from Andrographis stenophylla leaf.

  20. Source rack reload of the Tunisian gamma irradiation facility using Monte Carlo method

    International Nuclear Information System (INIS)

    Gharbi, Foued; Kadri, Omrane

    2005-01-01

    This work presents a Monte Carlo study of the cylindrical source rack geometry of the tunisian gamma irradiation facility, using Ge ant code of CERN. The study investigates the question of the reload of the source rack. studied configurations consist on housing four new pencils, two in the upper and two in the lower cylinder of the source rack. global dose rate uniformity inside a ''dummy's' product for the case of routine and non routine irradiation and as function of the product bulk density was calculated for eight hypothetical configurations. the same calculation was also performed for both of the original and the ideal, but not practical configuration. It was shown that hypothetical cases produced dose uniformity variations, according to product density, that were statistically no different than the original and the ideal configurations and that reload procedure can not improve the irradiation quality inside the tunisian facility

  1. Lessons Learned "Establishing an Electrically Safe Work Condition" Specifically related to Racking Electrical Breakers

    Energy Technology Data Exchange (ETDEWEB)

    Martinez, Tommy Robert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Romero, Philbert Roland [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Garcia, Samuel Anthony [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-11-09

    During low voltage electrical equipment maintenance, a bad breaker was identified. The breaker was racked out from the substation cubicle without following the hazardous energy control process identified in the Integrated Work Document (IWD). The IWD required the substation to be in an electrically safe work condition prior to racking the breaker. Per NFPA 70E requirements, electrical equipment shall be put into an electrically safe work condition before an employee performs work on or interacts with equipment in a manner that increases the likelihood of creating an arc flash. Racking in or out a breaker on an energized bus may increase the likelihood of creating an arc flash dependent on equipment conditions. A thorough risk assessment must be performed prior to performing such a task. The risk assessment determines the risk control measures to be put in place prior to performing the work. Electrical Safety Officers (ESO) can assist in performing risk assessments and incorporating risk control measures.

  2. Thermal analyses for the rack design with spent fuel pool during the loss of cooling accident

    Energy Technology Data Exchange (ETDEWEB)

    Yeh, C-L.; Chen, Y-S.; Chen, B-Y., E-mail: clinyeh@iner.gov.tw, E-mail: yschen@iner.gov.tw, E-mail: onepicemine@iner.gov.tw [Inst. of Nuclear Energy Research, Taoyuan County, Taiwan (China); Tseng, Y-S., E-mail: ystseng@mx.nthu.edu.tw [National Tsing Hua Univ., Engineering and System Science, Hsinchu, Taiwan (China); Wei, W-C., E-mail: hn150456@iner.gov.tw [Inst. of Nuclear Energy Research, Taoyuan County, Taiwan (China)

    2014-07-01

    Alternative fuel arrangements separating the latest fuels discharge from the reactor core are proposed, such as the 1x4 configuration in which the hot assembly is surrounded by 4 assemblies with much lower decay heat. For the rack design in the BWR spent fuel pool design, the lateral flow is eliminated by solid walls. In this study, cooling enhancement of splitting fuel rack is investigated using Computational Fluid Dynamics (CFD). The fuels in the pool are modeled by porous medium. Separating the fuel rack by a distance of 10 cm can lower the peak cladding temperature and the natural convection between the fuels and then earns more response time for the site people to implement necessary mitigation actions. (author)

  3. Antifungal activity of indigenous bacillus sp. isolate Q3 against marshmallow mycobiota

    Directory of Open Access Journals (Sweden)

    Jošić Dragana Lj.

    2011-01-01

    Full Text Available Marshmallow is a host of a number of saprophytic and parasitic fungi in Serbia. The seeds of marshmallow are contaminated with fungi from different genera, especially Alternaria and Fusarium, which significantly reduced seed germination and caused seedling decay. In this study we investigate antagnonism of indigenous Bacillus sp. isolate Q3 against marshmallow mycopopulation. Bacillus sp. Q3 was isolated from maize rhizosphere, characterized by polyphasic approch and tested for plant growth promoting treats. Bacillus sp. Q3 produced antifungal metabolites with growth inhibition activity against numerous fungi in dual culture: 61.8% of Alternaria alternata, 74.8% of Myrothecium verrucaria and 33.6% of Sclerotinia sclerotiorum. That effect could be caused by different antifungal metabolites including siderophores, hydrolytic enzymes, organic acids and indole acetic acid (IAA. Suppression of natural marshmallow seed infection by Q3 isolate was observed. The seeds were immersed in different concentrations of bacterial suspension during 2h and their infections by phytopathogenic fungi were estimated. The results showed significant reduction of seed infection by Alternaria spp. The presented results indicate possible application of this isolate as promising biological agent for control of marshmallow seed pathogenic fungi.

  4. Insecticidal Activity of Isolated Bacteria from Hyphantria cunea (Drury (Lepidoptera: Arctiidae

    Directory of Open Access Journals (Sweden)

    Nurcan Albayrak İskender

    2017-04-01

    Full Text Available The fall webworm (Hyphantria cunea is a polyphagous pest with numerous host plants. In the present study, the bacterial flora of H.cunea was investigated to identify new organisms that can be used as microbial control agent against the pest. Six bacteria were isolated and cultured from H. cunea. Some morphological, biochemical and other phenotypic characteristics (with API 20E, API 50 CH, API Staph and API Coryne kits of bacterial isolates were determined. In addition, 16S rRNA gene region was sequenced. As a result of the studies conducted, bacterial isolates were identified as Lysinibacillus sphaericus (Abk1, Bacillus amyloliquefaciens (Abk2, Staphylococcus sciuri (Abk4, Kocuria palustris (Abk6, Arthrobacter arilaitensis (Abk7 and Microbacterium oxydans (Abk8. All bacterial isolates were tested for 12 days against third-fourth instar larvae of H. cunea. The highest insecticidal activity was obtained from L. sphaericus (Abk1 with 30% after application (p<0.05. These results indicate that L. sphaericus (Abk1 can be taken into account in the microbial pest control of H. cunea. In the future, further studies will be conducted by using pathogenicity enrichment strategies of L. sphaericus (Abk1 (ex. combining with other entomopathogens or insecticides in order to increase the effectiveness on H. cunea.

  5. Isolation of dextran-hydrolyzing intestinal bacteria and characterization of their dextranolytic activities.

    Science.gov (United States)

    Kim, Jin Kyoung; Shin, So-Yeon; Moon, Jin Seok; Li, Ling; Cho, Seung Kee; Kim, Tae-Jip; Han, Nam Soo

    2015-06-01

    The aim of this study was to isolate dextran-hydrolyzing bacteria from the human intestines and to identify their dextranolytic enzymes. For this, dextranase-producing microorganisms were screened from fecal samples by using blue dextran-containing media. Colonies producing a decolorized zone were isolated and they were grouped using RAPD-PCR. 16S rRNA gene sequencing analysis revealed the isolates were Bacteroides (B.) thetaiotaomicron, B. ovatus, B. vulgatus, B. dorei, B. xylanisolvens, B. uniformis, and Veillonella (V.) rogosae. Thin layer chromatography analysis showed that the dextranases exhibit mainly endo-type activity and produce various oligosaccharides including isomaltose and isomaltotriose. Zymogram analysis demonstrated that enzymes localized mainly in the cell membrane fraction and the molecular weight was 50-70 kDa. When cultured in a dextran-containing medium, all strains isolated in this study produced short-chain fatty acids, with butyric acid as the major compound. This is the first study to report that human intestinal B. xylanisolvens, B. dorei, and V. rogosae metabolize dextran utilizing dextranolytic enzymes. © 2015 Wiley Periodicals, Inc.

  6. Isolation, Phylogenetic Analysis and Anti-infective Activity Screening of Marine Sponge-Associated Actinomycetes

    Directory of Open Access Journals (Sweden)

    Safwat Ahmed

    2010-02-01

    Full Text Available Terrestrial actinomycetes are noteworthy producers of a multitude of antibiotics, however the marine representatives are much less studied in this regard. In this study, 90 actinomycetes were isolated from 11 different species of marine sponges that had been collected from offshore Ras Mohamed (Egypt and from Rovinj (Croatia. Phylogenetic characterization of the isolates based on 16S rRNA gene sequencing supported their assignment to 18 different actinomycete genera representing seven different suborders. Fourteen putatively novel species were identified based on sequence similarity values below 98.2% to other strains in the NCBI database. A putative new genus related to Rubrobacter was isolated on M1 agar that had been amended with sponge extract, thus highlighting the need for innovative cultivation protocols. Testing for anti-infective activities was performed against clinically relevant, Gram-positive (Enterococcus faecalis, Staphylococcus aureus and Gram-negative (Escherichia coli, Pseudomonas aeruginosa bacteria, fungi (Candida albicans and human parasites (Leishmania major, Trypanosoma brucei. Bioactivities against these pathogens were documented for 10 actinomycete isolates. These results show a high diversity of actinomycetes associated with marine sponges as well as highlight their potential to produce anti-infective agents.

  7. Antifungal and antibacterial activity of endophytic penicillium species isolated from salvadora species

    International Nuclear Information System (INIS)

    Korejo, F.; Shafique, H.A.; Haque, S.E.; Ali, S.A.

    2014-01-01

    Salvadora persica and S. S.oleoides are facultative holophytic plants, well known as miswak, are traditionally used to ensure oral hygiene among Muslim people in Asian and African counties. Species of Salvadora have a number of proven pharmacological importance. Besides, terrestrial fungi endophytic fungi are also gaining importance for the isolation of bioactive compounds. In this study 74 samples (root, shoot and leaves) from S. persica and S. oleoides were examined for endophytic fungi, 22 samples showed presence of Penicillium spp., 48 were found positive for aspergilli, whereas 10 samples showed infection of Fusarium solani, 4 were found infected with Macrophomina phaseolina and one with Rhizoctonia solani. Most of the Penicillium isolated were identified as P. restrictum, P. citrinum and P. canescens. In dual culture plate assay out of four Penicillium isolates tested, P. citrinum and one isolate of P. restrictum caused growth inhibition of all four test root rotting fungi, Fusarium solani, F. oxysporum, Macrophomina phaseolina and Rhizoctonia solani. Culture filtrates of Penicillium spp., were also evaluated against four common laboratory bacteria namely Bacillus subtilis, Staphylococcus aureus, Salmonella typhimurium and Escherichia coli and above mentioned root rotting fungi. Culture filtrates of endophytic Penicillium spp., also showed significant antibacterial and antifungal activity. Secondary metabolites of endophytic Penicillium spp., offer an exciting area of research for the discovery of novel antimicrobial compounds. (author)

  8. Antitumor activity of extract and isolated compounds from Drechslera rostrata and Eurotium tonophilum

    Directory of Open Access Journals (Sweden)

    Fatmah A.S. Alasmary

    2018-02-01

    Full Text Available Total extracts of Drechslera rostrata and Eurotium tonophilum in addition of two isolated compounds from their cultures [di-2-ethylhexyl phthalate (H1 and 1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinone (H2] were tested for their antitumor activity using four human carcinoma cell lines. Antitumor activity was assessed by performing MTT assay to check the % cell viability. The % viability of HCT-116 (colon carcinoma, HeLa (cervical carcinoma, HEp-2 (larynx carcinoma and HepG-2 (hepatocellular carcinoma cells decreased after treatment with Drechslera rostrata and Eurotium tonophilum extracts, these effects were ranged from 059.0 ±  0.1 to 217.0  ±  0.3 µg/ml on all types of cancer cells. The best activity was recorded for Eurotium tonpholium extract (054.5 ± 0.3, 059.0 ± 0.5 and 059.0 ± 0.1 for HEp-2, Hela, and HepG-2 respectively. The isolated compounds (H1&H2 were found to be responsible about the activities because they recorded the lowest IC50 on tested cell lines with range of 9.5–20.3 μg/ml. Vinblastine sulphate was used as a reference standard and showed in vitro anticancer activity. This study demonstrated that all extracts and isolated compounds have antitumor activity against HCT-116, HeLa, HEp-2 and HepG-2 cells.

  9. Isolation of Abscisic Acid from Korean Acacia Honey with Anti-Helicobacter pylori Activity.

    Science.gov (United States)

    Kim, SeGun; Hong, InPyo; Woo, SoonOk; Jang, HyeRi; Pak, SokCheon; Han, SangMi

    2017-07-01

    Helicobacter pylori ( H. pylori ) is linked to the development of the majority of peptic ulcers and some types of gastric cancers, and its antibiotic resistance is currently found worldwide. This study is aimed at evaluating the anti- H. pylori activity of Korean acacia honey and isolating the related active components using organic solvents. The crude acacia honey was extracted with n -hexane, dichloromethane, ethyl acetate (EtOAc), and n -butanol. The EtOAc extract was subjected to octadecyl-silica chromatography. The extracts and fractions were then examined for anti- H. pylori activity using the agar well diffusion method. The antimicrobial activity of abscisic acid against H. pylori was investigated by determining the minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and by performing a time-kill assay. Abscisic acid related to the botanical origins of acacia honey from Korea has been analyzed using ultra-performance liquid chromatography. The MICs and MBCs of abscisic acid were 2.7 ± 1.3 and 6.9 ± 1.9 μg/mL, respectively. The bactericidal activity of abscisic acid (at 10.8 μg/mL corresponding to 4 × MIC) killed the organism within 36-72 h. These results suggest that abscisic acid isolated from Korean acacia honey has antibacterial activity against H. pylori . Abscisic acid isolated from Korean acacia honey can be therapeutic and may be further exploited as a potential lead candidate for the development of treatments for H. pylori -induced infections. The crude acacia honey was extracted with n -hexane, dichloromethane, EtOAc, and n -butanolThe EtOAc extract yielded eight fractions and four subfractions were subsequently obtained chromatographicallyAbscisic acid was isolated from one subfractionAll the solvent extracts and fractions showed antibacterial activity against H. pylori Abscisic acid exhibited antibacterial activity against H. pylori . Abbreviations used: MeOH: Methanol; EtOAc: Ethyl acetate; TSB: Trypticase

  10. Study on a new antifungal antibiotic, yimeimycin--isolation, structure elucidation and biological activities

    International Nuclear Information System (INIS)

    Shi Yuefeng; Sang Jinlong; Zhu Lihong; Li Xiaohui; Wu Jian

    2004-01-01

    Strain HA-8416, the producer of yimeimycin, was isolated from a soil sample collected in Hangzhou, Zhejiang province, China. Based on the investigation of morphological, cultural, physiological and biochemical characteristic as well as the cell wall chemical composition, strain HA8416 is extremely similar to Streptomyces hygrospinosus SF-104, and named Streptomyces hygrospinosus var tianmushanensis n. var. Sand et al. By means of spectroscopic analysis (UV, 1 H-NMR, DEPT CNMR and H-H COSY), yimeimycin was identified as a new antibiotic of the nucleoside family. Yimeimeycin appeared no activities against G + /G-bacteria, but was active against the fungi, Sphaerotheca cucurbitae, Pellicularia sasakii, Colletotrichum orbiculare, especially

  11. Enzymatic degradation of cellulose for thermophilic actinomycete: isolation, characterization and cellulolytic activity determination

    Directory of Open Access Journals (Sweden)

    Pablo Ramírez

    2013-06-01

    Full Text Available One hundred and forty five cellulolytic thermophilic actinomycete strains were isolated from 71 compost, soil, hay and dung samples. Streptomyces sp. (50,63%, Thermomonospora curvata (15,82%, T. chromogena (13,92%, and other species were identified. Endoglucanase, exoglucanase and β-glucosidase activities were evaluated from 10 cellulolytic actinomycete strains. Among these the Streptomyces sp. 7CMC10 strain showed the biggest activity levels corresponding to 20,14; 2,61 and 5,40 UI/mg of protein, respectively.

  12. In vitro culture of functionally active buffalo hepatocytes isolated by using a simplified manual perfusion method.

    Directory of Open Access Journals (Sweden)

    Santanu Panda

    Full Text Available In farm animals, there is no suitable cell line available to understand liver-specific functions. This has limited our understanding of liver function and metabolism in farm animals. Culturing and maintenance of functionally active hepatocytes is difficult, since they survive no more than few days. Establishing primary culture of hepatocytes can help in studying cellular metabolism, drug toxicity, hepatocyte specific gene function and regulation. Here we provide a simple in vitro method for isolation and short-term culture of functionally active buffalo hepatocytes.Buffalo hepatocytes were isolated from caudate lobes by using manual enzymatic perfusion and mechanical disruption of liver tissue. Hepatocyte yield was (5.3 ± 0.66×107 cells per gram of liver tissue with a viability of 82.3 ± 3.5%. Freshly isolated hepatocytes were spherical with well contrasted border. After 24 hours of seeding onto fibroblast feeder layer and different extracellular matrices like dry collagen, matrigel and sandwich collagen coated plates, hepatocytes formed confluent monolayer with frequent clusters. Cultured hepatocytes exhibited typical cuboidal and polygonal shape with restored cellular polarity. Cells expressed hepatocyte-specific marker genes or proteins like albumin, hepatocyte nuclear factor 4α, glucose-6-phosphatase, tyrosine aminotransferase, cytochromes, cytokeratin and α1-antitrypsin. Hepatocytes could be immunostained with anti-cytokeratins, anti-albumin and anti α1-antitrypsin antibodies. Abundant lipid droplets were detected in the cytosol of hepatocytes using oil red stain. In vitro cultured hepatocytes could be grown for five days and maintained for up to nine days on buffalo skin fibroblast feeder layer. Cultured hepatocytes were viable for functional studies.We developed a convenient and cost effective technique for hepatocytes isolation for short-term culture that exhibited morphological and functional characteristics of active hepatocytes

  13. Antibacterial Activities of Endophytic Bacteria Isolated from Taxus brevifolia Against Foodborne Pathogenic Bacteria.

    Science.gov (United States)

    Islam, Nurul; Choi, Jaehyuk; Baek, Kwang-Hyun

    2018-05-01

    Endophytes are a potential source of novel bioactive compounds with medicinal properties. In this study, 41 endophytic bacteria (EB) were isolated from tissues of a medicinally important plant Taxus brevifolia (Pacific yew). The objective was to screen all the EB isolates for their antibacterial effects against five foodborne pathogenic bacteria: Bacillus cereus ATCC10876, Staphylococcus aureus ATCC12600, Listeria monocytogenes ATCC19115, Escherichia coli ATCC43890, and Salmonella Typhimurium ATCC19585. Among the EB isolates, T. brevifolia seed (TbS)-8, T. brevifolia fleshy part of fruit (TbFl)-10, T. brevifolia leaf (TbL)-22, TbS-29, and TbL-34 exerted significant antibacterial activity against the tested foodborne pathogens. Especially TbFl-10 showed the highest antibacterial activity against all the tested bacteria and was identified as Paenibacillus kribbensis (Pk). Furthermore, an ethyl acetate extract of Pk-TbFl-10 possessed antibacterial activities against the tested five foodborne pathogenic bacteria, with zones of inhibition from 15.71 ± 2.85 to 13.01 ± 2.12 mm. Scanning electron microscopy analysis revealed ruptured, lysed, shrunk, and swollen cells of all the tested foodborne pathogens treated with the ethyl acetate extract of Pk-TbFl-10, suggesting that a metabolite(s) of Pk-TbFl-10 penetrates the cell membrane and causes cell lysis leading to cell death. Our results indicate that Pk-TbFl-10 isolated from T. brevifolia can serve as a novel source of natural antibacterial agents against foodborne pathogenic bacteria, with potential applications in the pharmaceutical industry.

  14. The diversity, extracellular enzymatic activities and photoprotective compounds of yeasts isolated in Antarctica

    Directory of Open Access Journals (Sweden)

    Aline B. M Vaz

    2011-09-01

    Full Text Available The diversity of yeasts collected from different sites in Antarctica (Admiralty Bay, King George Island and Port Foster Bay and Deception Island and their ability to produce extracellular enzymes and mycosporines were studied. Samples were collected during the austral summer season, between November 2006 and January 2007, from the rhizosphere of Deschampsia antarctica, ornithogenic (penguin guano soil, soil, marine and lake sediments, marine water and freshwater from lakes. A total of 89 isolates belonging to the following genera were recovered: Bensingtonia, Candida, Cryptococcus, Debaryomyces, Dioszegia, Exophiala, Filobasidium, Issatchenkia (Pichia, Kodamaea, Leucosporidium, Leucosporidiella, Metschnikowia, Nadsonia, Pichia, Rhodotorula, and Sporidiobolus, and the yeast-like fungi Aureobasidium, Leuconeurospora and Microglossum. Cryptococcus victoriae was the most frequently identified species. Several species isolated in our study have been previously reported to be Antarctic psychophilic yeasts, including Cr. antarcticus, Cr. victoriae, Dioszegia hungarica and Leucosporidium scottii. The cosmopolitan yeast species A. pullulans, C. zeylanoides, D. hansenii, I. orientalis, K. ohmeri, P. guilliermondii, Rh. mucilaginosa, and S. salmonicolor were also isolated. Five possible new species were identified. Sixty percent of the yeasts had at least one detectable extracellular enzymatic activity. Cryptococcus antarcticus, D. aurantiaca, D. crocea, D. hungarica, Dioszegia sp., E. xenobiotica, Rh. glaciales, Rh. laryngis, Microglossum sp. 1 and Microglossum sp. 2 produced mycosporines. Of the yeast isolates, 41.7% produced pigments and/or mycosporines and could be considered adapted to survive in Antarctica. Most of the yeasts had extracellular enzymatic activities at 4ºC and 20ºC, indicating that they could be metabolically active in the sampled substrates.

  15. Diversity and antimicrobial activity of culturable endophytic fungi isolated from moso bamboo seeds.

    Directory of Open Access Journals (Sweden)

    Xiao-Ye Shen

    Full Text Available Bamboos, regarded as therapeutic agents in ethnomedicine, have been used to inhibit inflammation and enhance natural immunity for a long time in Asia, and there are many bamboo associated fungi with medical and edible value. In the present study, a total of 350 fungal strains were isolated from the uncommon moso bamboo (Phyllostachys edulis seeds for the first time. The molecular diversity of these endophytic fungi was investigated and bioactive compound producers were screened for the first time. All the fungal endophytes were categorized into 69 morphotypes according to culturable characteristics and their internal transcriber spacer (ITS regions were analyzed by BLAST search with the NCBI database. The fungal isolates showed high diversity and were divided in Ascomycota (98.0% and Basidiomycota (2.0%, including at least 19 genera in nine orders. Four particular genera were considered to be newly recorded bambusicolous fungi, including Leptosphaerulina, Simplicillium, Sebacina and an unknown genus in Basidiomycetes. Furthermore, inhibitory effects against clinical pathogens and phytopathogens were screened preliminarily and strains B09 (Cladosporium sp., B34 (Curvularia sp., B35 (undefined genus 1, B38 (Penicillium sp. and zzz816 (Shiraia sp. displayed broad-spectrum activity against clinical bacteria and yeasts by the agar diffusion method. The crude extracts of isolates B09, B34, B35, B38 and zzz816 under submerged fermentation, also demonstrated various levels of bioactivities against bambusicolous pathogenic fungi. This study is the first report on the antimicrobial activity of endophytic fungi associated with moso bamboo seeds, and the results show that they could be exploited as a potential source of bioactive compounds and plant defense activators. In addition, it is the first time that strains of Shiraia sp. have been isolated and cultured from moso bamboo seeds, and one of them (zzz816 could produce hypocrellin A at high yield, which

  16. In vitro antimicrobial activity of ten medicinal plants against clinical isolates of oral cancer cases.

    Science.gov (United States)

    Panghal, Manju; Kaushal, Vivek; Yadav, Jaya P

    2011-05-20

    Suppression of immune system in treated cancer patients may lead to secondary infections that obviate the need of antibiotics. In the present study, an attempt was made to understand the occurrence of secondary infections in immuno-suppressed patients along with herbal control of these infections with the following objectives to: (a) isolate the microbial species from the treated oral cancer patients along with the estimation of absolute neutrophile counts of patients (b) assess the in vitro antimicrobial activity medicinal plants against the above clinical isolates. Blood and oral swab cultures were taken from 40 oral cancer patients undergoing treatment in the radiotherapy unit of Regional Cancer Institute, Pt. B.D.S. Health University,Rohtak, Haryana. Clinical isolates were identified by following general microbiological, staining and biochemical methods. The absolute neutrophile counts were done by following the standard methods. The medicinal plants selected for antimicrobial activity analysis were Asphodelus tenuifolius Cav., Asparagus racemosus Willd., Balanites aegyptiaca L., Cestrum diurnum L., Cordia dichotoma G. Forst, Eclipta alba L., Murraya koenigii (L.) Spreng. , Pedalium murex L., Ricinus communis L. and Trigonella foenum graecum L. The antimicrobial efficacy of medicinal plants was evaluated by modified Kirby-Bauer disc diffusion method. MIC and MFC were investigated by serial two fold microbroth dilution method. Prevalent bacterial pathogens isolated were Staphylococcus aureus (23.2%), Escherichia coli (15.62%), Staphylococcus epidermidis (12.5%), Pseudomonas aeruginosa (9.37%), Klebsiella pneumonia (7.81%), Proteus mirabilis (3.6%), Proteus vulgaris (4.2%) and the fungal pathogens were Candida albicans (14.6%), Aspergillus fumigatus (9.37%). Out of 40 cases, 35 (87.5%) were observed as neutropenic. Eight medicinal plants (A. tenuifolius, A. racemosus, B. aegyptiaca, E. alba, M. koenigii, P. murex R. communis and T. foenum graecum) showed

  17. Isolation of microbial native Stumps with cellulolytic activity of a compost process

    International Nuclear Information System (INIS)

    Jaramillo G, Marisol; Ruiz V Orlando Simon; Yepes P, Maria del Socorro; Montoya C, Olga Ines

    2003-01-01

    The isolation, selection adaptation and handling of native microorganisms coming from organic waste are an alternative to avoid the accumulation and the lack of the proper use of these undesirable materials. This organic waste is a source for obtaining microbial strains, which are potentially producers of Industrial enzymes and, at the same time, it works as substrate so that these organisms can transform it into compost or organic manure. In this work, 39 native strains of microorganisms with potential cellulolytic activity coming from the organic waste of the urban and rural sector, from the Compost Plant of Marinilla Antioquia) municipality, were isolated, evaluated and purified. The waste was previously selected and then submitted to an aerobic degradation or compost. The microbial strains were isolated in a selective medium with carboxymethyl cellulose (CMC), of the phases mesophile, termophile, cooling and maturation of the compost process. Eighty-two percent (82%)of the obtained colonies were identified, in principle as Bacillus, because of their morphology and their reaction to the Gram coloration. The fungi population was seen only during the cooling phase. Then, the potential cellulolytic activity was evaluated qualitatively in a solid medium with the Congo Red coloration, with which the Beta-endoglucanase activity was evaluated through the formation of clarified zones. Such staining was applied in two mediums with CMC with and without glucose It was observed that 33.3% of the isolated organisms produced the enzyme In both mediums; however, 25.6% of microorganisms did not show the production of this enzyme, and only 15.8% did not require the inducers to produce it

  18. In vitro antimicrobial activity of ten medicinal plants against clinical isolates of oral cancer cases

    Science.gov (United States)

    2011-01-01

    Background Suppression of immune system in treated cancer patients may lead to secondary infections that obviate the need of antibiotics. In the present study, an attempt was made to understand the occurrence of secondary infections in immuno-suppressed patients along with herbal control of these infections with the following objectives to: (a) isolate the microbial species from the treated oral cancer patients along with the estimation of absolute neutrophile counts of patients (b) assess the in vitro antimicrobial activity medicinal plants against the above clinical isolates. Methods Blood and oral swab cultures were taken from 40 oral cancer patients undergoing treatment in the radiotherapy unit of Regional Cancer Institute, Pt. B.D.S. Health University, Rohtak, Haryana. Clinical isolates were identified by following general microbiological, staining and biochemical methods. The absolute neutrophile counts were done by following the standard methods. The medicinal plants selected for antimicrobial activity analysis were Asphodelus tenuifolius Cav., Asparagus racemosus Willd., Balanites aegyptiaca L., Cestrum diurnum L., Cordia dichotoma G. Forst, Eclipta alba L., Murraya koenigii (L.) Spreng. , Pedalium murex L., Ricinus communis L. and Trigonella foenum graecum L. The antimicrobial efficacy of medicinal plants was evaluated by modified Kirby-Bauer disc diffusion method. MIC and MFC were investigated by serial two fold microbroth dilution method. Results Prevalent bacterial pathogens isolated were Staphylococcus aureus (23.2%), Escherichia coli (15.62%), Staphylococcus epidermidis (12.5%), Pseudomonas aeruginosa (9.37%), Klebsiella pneumonia (7.81%), Proteus mirabilis (3.6%), Proteus vulgaris (4.2%) and the fungal pathogens were Candida albicans (14.6%), Aspergillus fumigatus (9.37%). Out of 40 cases, 35 (87.5%) were observed as neutropenic. Eight medicinal plants (A. tenuifolius, A. racemosus, B. aegyptiaca, E. alba, M. koenigii, P. murex R. communis and T

  19. Sputum Active Polymyxin Lipopeptides: Activity against Cystic Fibrosis Pseudomonas aeruginosa Isolates and Their Interactions with Sputum Biomolecules.

    Science.gov (United States)

    Schneider-Futschik, Elena K; Paulin, Olivia K A; Hoyer, Daniel; Roberts, Kade D; Ziogas, James; Baker, Mark A; Karas, John; Li, Jian; Velkov, Tony

    2018-05-11

    The mucoid biofilm mode of growth of Pseudomonas aeruginosa ( P. aeruginosa) in the lungs of cystic fibrosis patients makes eradication of infections with antibiotic therapy very difficult. The lipopeptide antibiotics polymyxin B and colistin are currently the last-resort therapies for infections caused by multidrug-resistant P. aeruginosa. In the present study, we investigated the antibacterial activity of a series of polymyxin lipopeptides (polymyxin B, colistin, FADDI-003, octapeptin A 3 , and polymyxin A 2 ) against a panel of polymyxin-susceptible and polymyxin-resistant P. aeruginosa cystic fibrosis isolates grown under planktonic or biofilm conditions in artificial sputum and their interactions with sputum component biomolecules. In sputum media under planktonic conditions, the lipopeptides FADDI-003 and octapeptin A 3 displayed very promising activity against the polymyxin-resistant isolate FADDI-PA066 (polymyxin B minimum inhibitory concentration (MIC) = 32 mg/L), while retaining their activity against the polymyxin-sensitive strains FADDI-PA021 (polymyxin B MIC = 1 mg/L) and FADDI-PA020 (polymyxin B MIC = 2 mg/L). Polymyxin A 2 was only effective against the polymyxin-sensitive isolates. However, under biofilm growth conditions, the hydrophobic lipopeptide FADDI-003 was inactive compared to the more hydrophilic lipopeptides, octapeptin A 3 , polymyxin A 2 , polymyxin B, and colistin. Transmission electron micrographs revealed octapeptin A 3 caused reduction in the cell numbers in biofilm as well as biofilm disruption/"antibiofilm" activity. We therefore assessed the interactions of the lipopeptides with the component sputum biomolecules, mucin, deoxyribonucleic acid (DNA), surfactant, F-actin, lipopolysaccharide, and phospholipids. We observed the general trend that sputum biomolecules reduce lipopeptide antibacterial activity. Collectively, our data suggests that, in the airways, lipopeptide binding to component sputum biomolecules may reduce

  20. Cytotoxic activity of isolated constituents from leaves of Premna serratifolia on MCF-7 and HT-29 cell lines

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    Mahesh Biradi

    2015-03-01

    Full Text Available Premna serratifolia (Syn: Premna integrifolia is an important medicinal herb known as “Agnimantha” in Ayurveda and traditionally used for anticancer activity. The objective of present study was to isolate the cytotoxic phytoconstituents from the n-hexane soluble fraction of P. serratifolia leaf extract. Unsaponifiable portion of n-hexane soluble fraction was subjected to silica based column chromatography. The major constituents present in all the sub-fractions were identified by TLC and phytochemical tests. Two constituents were isolated and they were purified. Sub-fractions with isolates were tested for cytotoxic effect by BSL bioassay. Two isolates were found to be active and which were tested on cancer cell lines MCF-7 and HT-29 for their cytotoxicity. Among two isolates, one compound has shown significant cytotoxicity. From the results we conclude that the plant isolates showed cytotoxicity against selected human cancer cell lines.

  1. Dioscorin isolated from Dioscorea alata activates TLR4-signaling pathways and induces cytokine expression in macrophages.

    Science.gov (United States)

    Fu, Shu-Ling; Hsu, Ya-Hui; Lee, Pei-Yeh; Hou, Wen-Chi; Hung, Ling-Chien; Lin, Chao-Hsiung; Chen, Chiu-Ming; Huang, Yu-Jing

    2006-01-06

    The Toll-like receptor 4 (TLR4)-signaling pathway is crucial for activating both innate and adaptive immunity. TLR4 is a promising molecular target for immune-modulating drugs, and TLR4 agonists are of therapeutic potential for treating immune diseases and cancers. Several medicinal herb-derived components have recently been reported to act via TLR4-dependent pathways, suggesting that medicinal plants are potential resources for identifying TLR4 activators. We have applied a screening procedure to systematically identify herbal constituents that activate TLR4. To exclude possible LPS contamination in these plant-derived components, a LPS inhibitor, polymyxin B, was added during screening. One of the plant components we identified from the screening was dioscorin, the glycoprotein isolated from Dioscorea alata. It induced TLR4-downstream cytokine expression in bone marrow cells isolated from TLR4-functional C3H/HeN mice but not from TLR4-defective C3H/HeJ mice. Dioscorin also stimulated multiple signaling molecules (NF-kappaB, ERK, JNK, and p38) and induced the expression of cytokines (TNF-alpha, IL-1beta, and IL-6) in murine RAW 264.7 macrophages. Furthermore, the ERK, p38, JNK, and NF-kappaB-mediated pathways are all involved in dioscorin-mediated TNF-alpha production. In summary, our results demonstrate that dioscorin is a novel TLR4 activator and induces macrophage activation via typical TLR4-signaling pathways.

  2. Antibacterial activity of Iranian medicinal plants against Streptococcus iniae isolated from rainbow trout (Oncorhynchus mykiss

    Directory of Open Access Journals (Sweden)

    Pirbalouti Ghasemi Abdollah

    2011-01-01

    Full Text Available Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems. Ten Iranian medicinal plants were assessed for their antimicrobial activity against Streptococcus iniae isolates obtained from diseased Oncorhynchus mykiss (Salmonidae; Walbaum, 1972 collected from fish farms in Iran. The antibacterial activity of ethanol extracts of Punica granatum, Quercus branti, Glycyrrhiza glabra and essential oils of Heracleum lasiopetalum, Satureja bachtiarica, Thymus daenensis, Myrtus communis, Echinophora platyloba, Kelussia odoratissima and Stachys lavandulifolia against Steptococcus iniae was evaluated by disc diffusion and serial dilution assays. Most of the extracts and essential oils showed a relatively high antibacterial activity against Streptococcus iniae. Of the plants studied, the most active extracts were those obtained from the essential oils of Satureja bachtiarica, Echinophora platyloba, Thymus daenensis and the ethanol extract of Quercus branti. Some of the extracts were active against Streptococcus iniae. Two essential oils showed lower MIC values; Heracleum lasiopetalum (78 μg/ml and Satureja bachtiarica (39 μg/ml. The essential oil of Satureja bachtiarica could be an important source of antibacterial compounds against the Streptococcus iniae isolated from rainbow trout.

  3. SCREENING OF ANTIMICROBIAL ACTIVITY AND GENES CODING POLYKETIDE SYNTHETASE AND NONRIBOSOMAL PEPTIDE SYNTHETASE OF ACTINOMYCETE ISOLATES

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    Silvia Kovácsová

    2013-12-01

    Full Text Available The aim of this study was to observe antimicrobial activity using agar plate diffusion method and screening genes coding polyketide synthetase (PKS-I and nonribosomal peptide synthetase (NRPS from actinomycetes. A total of 105 actinomycete strains were isolated from arable soil. Antimicrobial activity was demonstrated at 54 strains against at least 1 of total 12 indicator organisms. Antifungal properties were recorded more often than antibacterial properties. The presence of PKS-I and NRPS genes were founded at 61 of total 105 strains. The number of strains with mentioned biosynthetic enzyme gene fragments matching the anticipated length were 19 (18% and 50 (47% respectively. Overall, five actinomycete strains carried all the biosynthetical genes, yet no antimicrobial activity was found against any of tested pathogens. On the other hand, twenty-one strains showed antimicrobial activity even though we were not able to amplify any of the PKS or NRPS genes from them. Combination of the two methods showed broad-spectrum antimicrobial activity of actinomycetes isolated from arable soil, which indicate that actinomycetes are valuable reservoirs of novel bioactive compounds.

  4. Lessons Learned from the Crew Health Care System (CHeCS) Rack 1 Environmental Control and Life Support (ECLS) Design

    Science.gov (United States)

    Williams, David E.

    2006-01-01

    This paper will provide an overview of the International Space Station (ISS) Environmental Control and Life Support (ECLS) design of the Crew Health Care System (CHeCS) Rack 1 and it will document some of the lessons that have been learned to date for the ECLS equipment in this rack.

  5. Can an off-the-rack orthotic stiletto alter pressure and comfort scores in the forefoot, arch and heel?

    DEFF Research Database (Denmark)

    Penny, Jeannette Østergaard; Speedtsberg, Merete Brink; Kallemose, Thomas

    2018-01-01

    MTH2+3 pressure (p rack orthotic stilettos can notably reduce...... plantar pressures and improve forefoot and heel comfort during everyday use. Practitioner Summary: Off-the-rack orthotic stilettos with built-in metatarsal pad, arch support and heel caps can lower the pressure under the heel and forefoot in comparison with a standard stiletto and can improve comfort...

  6. 40 CFR Table 9 to Subpart Eeee of... - Continuous Compliance With Operating Limits-High Throughput Transfer Racks

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Continuous Compliance With Operating Limits-High Throughput Transfer Racks 9 Table 9 to Subpart EEEE of Part 63 Protection of Environment...—Continuous Compliance With Operating Limits—High Throughput Transfer Racks As stated in §§ 63.2378(a) and (b...

  7. 77 FR 27029 - Certain Kitchen Shelving and Racks From the People's Republic of China: Extension of Time Limit...

    Science.gov (United States)

    2012-05-08

    ... DEPARTMENT OF COMMERCE International Trade Administration [C-570-942] Certain Kitchen Shelving and Racks From the People's Republic of China: Extension of Time Limit for Preliminary Results of... certain kitchen appliance shelving and racks from the People's Republic of China, covering the review...

  8. Trypanocidal and leishmanicidal activities of flavonoids isolated from Stevia satureiifolia var. satureiifolia.

    Science.gov (United States)

    Beer, María Florencia; Frank, Fernanda Maria; Germán Elso, Orlando; Ernesto Bivona, Augusto; Cerny, Natacha; Giberti, Gustavo; Luis Malchiodi, Emilio; Susana Martino, Virginia; Alonso, María Rosario; Patricia Sülsen, Valeria; Cazorla, Silvia Ines

    2016-10-01

    Context Chagas' disease and leishmaniasis produce significant disability and mortality with great social and economic impact. The genus Stevia (Asteraceae) is a potential source of antiprotozoal compounds. Objective Aerial parts of four Stevia species were screened on Trypanosoma cruzi. Stevia satureiifolia (Lam.) Sch. Bip. var. satureiifolia (Asteraceae) dichloromethane extract was selected for a bioassay-guided fractionation in order to isolate its active compounds. Additionally, the antileishmanial activity and the cytotoxicity of these compounds on mammalian cells were assessed. Materials and methods The dichloromethane extract was fractionated by column chromatography. The isolated compounds were evaluated using concentrations of 0-100 μg/mL on T. cruzi epimastigotes and on Leishmania braziliensis promastigotes for 72 h, on trypomastigotes and amastigotes of T. cruzi for 24 h and 120 h, respectively. The compounds' cytotoxicity (12.5-500 μg/mL) was assessed on Vero cells by the MTT assay. The structure elucidation of each compound was performed by spectroscopic methods and HPLC analysis. Results The dichloromethane extracts of Stevia species showed significant activity on T. cruzi epimastigotes. The flavonoids eupatorin (1.3%), cirsimaritin (1.9%) and 5-desmethylsinensetin (1.5%) were isolated from S. satureiifolia var. satureiifolia extract. Eupatorin and 5-desmethylsinensetin showed IC50 values of 0.2 and 0.4 μg/mL on T. cruzi epimastigotes and 61.8 and 75.1 μg/mL on trypomastigotes, respectively. The flavonoid 5-desmethylsinensetin showed moderate activity against T. cruzi amastigotes (IC50  value = 78.7 μg/mL) and was the most active compound on L. braziliensis promastigotes (IC50  value = 37.0 μg/mL). Neither of the flavonoids showed cytotoxicity on Vero cells, up to a concentration of 500 μg/mL.

  9. Enhancement of antibiotic activity by efflux inhibitors against multidrug resistant Mycobacterium tuberculosis clinical isolates from Brazil

    Directory of Open Access Journals (Sweden)

    Tatiane eCoelho

    2015-04-01

    Full Text Available Drug resistant tuberculosis continues to increase and new approaches for its treatment are necessary. The identification of M. tuberculosis clinical isolates presenting efflux as part of their resistant phenotype has a major impact in tuberculosis treatment. In this work, we used a checkerboard procedure combined with the tetrazolium microplate-based assay (TEMA to study single combinations between antituberculosis drugs and efflux inhibitors (EIs against multidrug resistant M. tuberculosis clinical isolates using the fully susceptible strain H37Rv as reference. Efflux activity was studied on a real-time basis by a fluorometric method that uses ethidium bromide as efflux substrate. Quantification of efflux pump genes mRNA transcriptional levels were performed by RT-qPCR. The fractional inhibitory concentrations (FIC indicated synergistic activity for the interactions between isoniazid, rifampicin, amikacin, ofloxacin, and ethidium bromide plus the EIs verapamil, thioridazine and chlorpromazine. The FICs ranged from 0.25, indicating a four-fold reduction on the MICs, to 0.015, 64-fold reduction. The detection of active efflux by real-time fluorometry showed that all strains presented intrinsic efflux activity that contributes to the overall resistance which can be inhibited in the presence of the EIs. The quantification of the mRNA levels of the most important efflux pump genes on these strains shows that they are intrinsically predisposed to expel toxic compounds as the exposure to subinhibitory concentrations of antibiotics were not necessary to increase the pump mRNA levels when compared with the non-exposed counterpart. The results obtained in this study confirm that the intrinsic efflux activity contributes to the overall resistance in multidrug resistant clinical isolates of M. tuberculosis and that the inhibition of efflux pumps by the EIs can enhance the clinical effect of antibiotics that are their substrates.

  10. Development of Antioxidant Activity during Milk Fermentation by Wild Isolates of Lactobacillus helveticus

    Directory of Open Access Journals (Sweden)

    Aazam Namdari

    2016-07-01

    Full Text Available Background and Objective: Oxidative stress, due to free radicals, brings injury to the body by attacking large molecules and cell organs, and is the main reason of many diseases. Fermentation of foods containing large amount of proteins such as milk by special species of lactic acid bacteria is a potential way in enhancement of the antioxidative activity of foods. This study aimed at evaluating non-common starter species isolates of Lactobacillus helveticus for their capability to produce fermented milk enriched in antioxidant peptides.Materials and Methods: Reconstituted skim milk (11% was inoculated with 7 wild isolates of Lactobacillus helveticus, and after 24 h fermentation at 37ºC, the samples were kept 4ºC and for 14 days. Viable cell number, acidification and proteolysis degree in the milk fermented by each isolate were assessed in 1, 7 and 14 days. Development of antioxidant activity was measured using DPPH and ABTS●+ radial scavenging activities during the storage period.Results and Conclusion: Though some slight strain-dependent differences were observed in growth, acidification and proteolysis, all the samples showed considerably strong antioxidant activity (at least 62.32±3.66% and 57.64±1.42% measured using DPPH and ABTS●+ radicals, respectively through the whole storage period. In vitro simulated gastrointestinal digestion indicated that DPPH radical-scavenging activity of the antioxidative peptidic supernatants was not affected significantly by consecutive pepsin-pancreatin hydrolysis in most of the samples. These evidences support Lactobacillus helveticus as a promising functional culture able to promote health benefits in dairy-based functional foods.Conflict of interest: The authors declare that there is no conflict of interest.

  11. Comparison of enzymatic activities in different Candida species isolated from women with vulvovaginitis.

    Science.gov (United States)

    Fatahinia, M; Halvaeezadeh, M; Rezaei-Matehkolaei, A

    2017-06-01

    Comparing the activities of secreted enzymes in different fungal species can improve our understanding of their pathogenic role. Secretion of various enzymes by Candida species has been considered for determination of their virulence in different Candida infections including vulvovaginitis. The aim of this study was to determine and compare the activity of secreted enzymes in Candidia strains isolated from women suspected to vulvovaginal candidiasis (VVC) and referred to some health centers in Khuzestan, Southwestern Iran. The vaginal secretion samples were taken by swap from 250 suspected women with symptoms of vulvovaginal candidiasis and cultured on CHROMagar Candida medium. Identification of the isolated Candida from culture positive samples performed by the color of colonies and some standard mycological procedures. Activities of phospholipase, hemolysin-α, hemolysin-β, esterase and proteinase were measured in vitro by standard laboratory protocols. The enzymatic activity index (EAI) was calculated for each enzyme in accordance to relevant protocols. Totally in eighty cases (32%), a Candida strain was isolated which found to be as 52 (65%) Candida albicans; 12 (15%) C. glabrata; 10 (12.5%) C. dubliniensis; 4 (5%) C. krusei, C. tropicalis and C. parapsilosis species (each=1; 1.3%). Among C. albicans strains, 89.1% produced all studied enzymes, while 86% of C. glabrata strains failed to produce proteinase and phospholipase. The EAIs in decreasing order were as hemolysin-β=0.2895, hemolysin-α=0.5420, esterase=0.5753, proteinase=0.7413, and phospholipase=0.7446, respectively. Activity of phospholipase, esterase and proteinase secreted by C. albicans and C. dubliniensis were significantly more than those released by C. glabrata and C. krusei, while 86% of C. glabrata strains did not show esterase activity. On the other hand, the activity rates of hemolysin α and β among all studied isolates were almost similar. In the present study, the prevalence

  12. Chemical constituents of Aspergillus sp EJC08 isolated as endophyte from Bauhinia guianensis and their antimicrobial activity.

    Science.gov (United States)

    Pinheiro, Eduardo A A; Carvalho, Josiwander M; Santos, Diellem C P dos; Feitosa, André O; Marinho, Patrícia S B; Guilhon, Giselle Maria S P; Santos, Lourivaldo S; Souza, Afonso L D de; Marinho, Andrey M R

    2013-01-01

    The present work reports the isolation of five compounds from Aspergillus sp EJC08 isolated as endophytic from Bauhinia guianensis, a tipical plant of the Amazon. The compounds ergosterol (1), ergosterol peroxide (2), mevalolactone (3), monomethylsulochrin (4) and trypacidin A (5) were isolated by chromatographic procedures and identified by spectral methods of 1D and 2D NMR and MS. Compounds 3, 4 and 5 were tested against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus and showed good activity.

  13. Evaluation of antifungal activity of standardized extract of Salvia rhytidea Benth. (Lamiaceae) against various Candida isolates.

    Science.gov (United States)

    Salari, S; Bakhshi, T; Sharififar, F; Naseri, A; Ghasemi Nejad Almani, P

    2016-12-01

    Salvia species have long been described in traditional medicine for various indications. Owing to the widespread use of this genus by ethnic populations, especially for various infections ranging from skin disease to gastrointestinal disorders, we were encouraged to determine whether Salvia rhytidea could be effective against fungal infections. Given the increased incidence of candidiasis in the past decade, limits on the use of antifungal drugs, emergence of azole-resistant Candida species and increased incidence of treatment failures, it is necessary to identify a novel agent with antifungal properties. Aim of the study was to evaluate the antifungal properties of S. rhytidea against various Candida isolates. In this study, at first rosmarinic acid content of plant extract was determined. A total of 96 Candida isolates were tested, including the following species: Candida albicans (n=42), Candida glabrata (n=16), Candida tropicalis (n=11), Candida krusei (n=9), Candida parapsilosis (n=9), Candida lusitaniae (n=7) and Candida guilliermondii (n=2). The in vitro antifungal activity of methanolic extracts of S. rhytidea Benth. was evaluated against Candida isolates and compared with that of the standard antifungal drug nystatin by using a broth microdilution method, according to CLSI. Phytochemical screening results showed that the methanolic extract of S. rhytidea Benth. was rich in flavonoids and tannins. The minimal inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of S. rhytidea Benth. ranged from 3.125 to>100μg/ml and 6.25 to>100μg/ml respectively. The growth inhibition value displayed that C. tropicalis, C. krusei and C. albicans isolates were most susceptible to S. rhytidea. Findings show that S. rhytidea possesses an antifungal effect against Candida isolates. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  14. Isolation and characterization of marine bacteria from macroalgae Gracilaria salicornia and Gelidium latifolium on agarolitic activity for bioethanol production

    Science.gov (United States)

    Kawaroe, M.; Pratiwi, I.; Sunudin, A.

    2017-05-01

    Gracilaria salicornia and Gelidium latifolium have high content of agar and potential to be use as raw material for bioethanol. In bioethanol production, one of the processes level is enzyme hydrolysis. Various microorganisms, one of which is bacteria, can carry out the enzyme hydrolysis. Bacteria that degrade the cell walls of macroalgae and produce an agarase enzyme called agarolytic bacteria. The purpose of this study was to isolate bacteria from macroalgae G. salicornia and G. latifolium, which has the highest agarase enzyme activities, and to obtain agarase enzyme characteristic for bioethanol production. There are two isolates bacteria resulted from G. salicornia that are N1 and N3 and there are two isolates from G. latifolium that are BSUC2 and BSUC4. The result of agarase enzyme qualitative test showed that isolates bacteria from G. latifolium were greater than G. salicornia. The highest agarolitic index of bacteria from G. salicornia produced by isolate N3 was 2.32 mm and isolate N3 was 2.27 mm. Bacteria from G. latifolium produced by isolate BSUC4 was 4.28 mm and isolate BSUC2 was 4.18 mm, respectively. Agarase enzyme activities from isolates N1 and N3 were optimum working at pH 7 and temperature 30 °C, while from isolates BSUC4 was optimum at pH 7 and temperature 50 °C. This is indicated that the four bacteria are appropriate to hydrolyze macro alga for bioethanol production.

  15. Antifungal Activity of Bacillus coagulans TQ33, Isolated from Skimmed Milk Powder, against Botrytis cinerea

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    Rui Feng Xiao

    2013-01-01

    Full Text Available Bacillus coagulans TQ33 is isolated from the skimmed milk powder and has a broad antifungal activity against pathogens such as Botrytis cinerea, Alternaria solani, Phytophthora drechsleri Tucker, Fusarium oxysporum and Glomerella cingulata. The characteristics of active antifungal substances produced by B. coagulans TQ33 and its antifungal effects against the growth of plant pathogenic fungi has been evaluated. The effect of pH, temperature and protease on the antifungal activity of B. coagulans TQ33 was determined. The results of partial characterization of the antifungal compound indicated that its activity is likely to be due to the production of a proteinaceous substance together with other substances. The greenhouse trials suggest that B. coagulans TQ33 has a great potential for the control of plant pathogenic fungi.

  16. Antimicrobial activity of Mimosa tenuiflora (Willd. Poir. from Northeast Brazil against clinical isolates of Staphylococcus aureus

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    Itácio Q. M. Padilha

    Full Text Available Mimosa tenuiflora is a native plant of Northeast Brazil where it is popularly known as ''jurema-preta'' and it is widely used in folk medicine. In this work the anti-Staphylococcus aureus activity of ethanol extract of M tenuiflora was evaluated by determination of minimum inhibitory concentration (MIC of clinical isolates by the agar dilution method, and by time-kill assay using a reference strain. MIC values against 30 isolates were 0,18 mg/mL (16/30 or 0,36 mg/mL (14/30, and also the reference strain. In the reference strains, at concentrations up to 4x MIC, only bacteriostatic effect was observed, but at 8x MIC a fast bactericidal effect was observed.

  17. Screening of medicinal plants for antibacterial activities on Staphylococcus aureus strains isolated from bovine mastitis

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    Marisa A. N. Diaz

    2010-08-01

    Full Text Available Staphylococcus aureus is the main causative agent of bovine mastitis. The activity of several extracts from ten medicinal plants traditionally used in Brazil as antiseptic was investigated against fifteen strains of Staphylococcus aureus isolated from animals with mastitis manifestation by the disc diffusion method and broth microdilution assay. The interference of the extracts on cell in the form of adherent colonies was also evaluated. MIC values ranged from 0.5 mg/mL to 1.0 mg/mL and biofilm inhibitory concentration (BIC were between 0.25 mg/mL and 0.8 mg/mL. Results revealed the potential of extracts of Senna macranthera, Artemisia absinthium, Cymbopogon nardus and Baccharis dracunculifolia as antibacterial agents against S. aureus strains isolated from bovine mastitis and support the possible use of these phytotherapic agents in the clinical management of the disease.

  18. In vitro activity of daptomycin against clinical isolates of Gram-positive bacteria.

    Science.gov (United States)

    Piper, Kerryl E; Steckelberg, James M; Patel, Robin

    2005-08-01

    We determined the activity of daptomycin, a recently FDA-approved antimicrobial agent, against clinical isolates of Gram-positive bacteria, including viridans group streptococci (16 Streptococcus mitis species group, 12 S. mutans species group, 9 S. anginosus species group, 8 S. sanguinis species group, 5 S. salivarius species group) from patients with infective endocarditis, 32 methicillin-resistant Staphylococcus aureus, 32 high-level penicillin-resistant Streptococcus pneumoniae, 38 vancomycin-resistant enterococci (including 1 linezolid-resistant isolate), and the following unusual Gram-positive bacteria: 3 Listeria monocytogenes, 4 Erysipelothrix rhusiopathiae, 9 Corynebacterium species, 10 Abiotrophia/Granulicatella species, 2 Rothia (Stomatococcus) mucilaginosus, and 4 Gemella morbillorum. Daptomycin minimum inhibitory concentration (MIC)(90) values for the viridans group streptococci, methicillin-resistant S. aureus, penicillin-resistant S. pneumoniae, and Enterococcus species were 0.5, 0.5, endocarditis as well as against several types of unusual Gram-positive bacteria that can cause endocarditis.

  19. Anti-ulcerogenic Activity of Extract and Some Isolated Flavonoids from Desmostachia bipinnata (L. Stapf

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    Amani, S. Awaad

    2008-08-01

    Full Text Available Five main flavonoid glycosides were isolated, for the first time, from the ethanol extract of Desmostachia bipinnata (L.Stapf ( Gramineae . They were identified as kaempferol(1, quercetin(2, quercetin-3-glucoside(3, trycin(4 and trycin-7-glucoside(5. The structure elucidation was based on UV, Electrospray ionization mass spectrometry (ESIMS, 1H and 13C NMR, proton- proton correlation spectroscopy ( 1H- 1H Cosy, distortionless enhancement by polarization transfer (DEPT, heteronuclear single quantum coherence (HSQC, and heteronuclear multiple bond correlations spectrum (HMBC. The total extract (200 and 300 mg/kg and two of the isolated compounds (trycin and trycin-7-glucoside.100 mg/kg each showed a very promising antiulcerogenic activity with curative ratios; 68.31, 70.54, 77.39 and 78.93%, respectively.

  20. The development of underwater remote cutting method for the disassembling of rotary specimen rack KRR-1 and 2

    Energy Technology Data Exchange (ETDEWEB)

    Lee, D. K.; Jung, K. H.; Lee, K. W.; Oh, W. J. [KAERI, Taejon (Korea, Republic of); Lee, K. Y. [Korea Institute of Industrial Technology, Kwangju (Korea, Republic of)

    2004-07-01

    The Rotary Specimen Racks (RSRs) were highly activated and then classified intermediate level radioactive waste for the decommissioning of KRR-1anel2. The RSR can be treated as low level radioactive waste after removing stainless steel parts. To reduce the volume of intermediate level radioactive waste, underwater cutting is required to separate stainless steel parts from RSR because of high radioactivity. In this study, the automatic remote cutting method was developed to disassemble RSR under water. For automatic remote cutting processes, a CAM (Computer Aided Manufacturing) system is employed. A computer inputs NC (Numerical Control) codes to the controller, which are based on CAM model, and the controller instructs the equipment to process according to NC codes automatically. And the cutting force model was improved to cut RSR stably. The automatic cutting was conducted using imitation of RSR and then it was resulted that the developed automatic cutting method can be safely disassemble stainless steel parts of RSR under water.

  1. Wound healing and protease inhibition activity of Bacoside-A, isolated from Bacopa monnieri wettest.

    Science.gov (United States)

    Sharath, R; Harish, B G; Krishna, V; Sathyanarayana, B N; Swamy, H M Kumara

    2010-08-01

    Bacopa monnieri (L.) Wettest. (Scrophulariaceae) is a well-known medicinal herb. In the Indian system of medicine it is known as Brahmi (Sanskrit) and Indian water hyssop. Methanolic extract of Bacopa monnieri and its isolated constituent Bacoside-A were screened for wound healing activity. Bacoside-A was screened for wound healing activity by excision, incision and dead space wound on Swiss albino rats. Significant wound healing activity was observed in both extract and the Bacoside-A treated groups. The SDS-PAGE caseinolytic zymogram analysis of inhibition of matrix metalloproteases (MMPs) enzyme from the excision wound by Bacoside-A, an isolated constituent, was done with the concentrations 100 and 200 micromg/ml. In Bacoside-A treated groups, epithelialization of the excision wound was faster with a high rate (18.30 +/- 0.01 days) of wound contraction. The tensile strength of the incision wound was increased (538.47 +/- 0.14 g) in the Bacoside-A treated group. In the dead space wound model, the weight of the granuloma was also increased (89.15 +/- 0.08 g). The histological examination of the granuloma tissue of the Bacoside-A treated group showed increased cross-linking of collagen fibers and absence of monocytes. The wound healing activity of Bacoside-A was more effective in various wound models compared to the standard skin ointment Nitrofurazone.

  2. Immunostimulatory and anti-metastatic activity of polysaccharides isolated from byproducts of the corn starch industry.

    Science.gov (United States)

    Lee, Sue Jung; Lee, Ho Sung; Kim, Sun Young; Shin, Kwang-Soon

    2018-02-01

    Corn steep liquor (CSL) is a major by-product of the corn steeping process that is utilized in the wet milling industry. To develop new physiologically active polysaccharides from CSL, polysaccharides were isolated and their innate immunostimulatory and anti-metastatic activities were investigated. Corn byproduct polysaccharides (CBP) were preferentially isolated from CSL and further separated into supernatant (CBP1S) and precipitate (CBP1P) fractions. The anti-complementary activity of CBP1S was more potent than CBP1P and CBP. In addition, CBP1S enhanced production of macrophage-stimulating cytokines (e.g., IL-6 and IL-12) and natural killer (NK) cell-activating substances (e.g., granzyme and interferon-γ). Further, CBP1S significantly inhibited lung metastasis at a dose of 1000μg per mouse in an experimental lung metastasis model. These results suggest that CBP1S seems to promote the inhibition of lung metastasis through a mechanism leading to stimulation of the innate immune system, and CBP1S could be used as immunostimulating agents and for industrial applications. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Evaluation of in vitro anti-proliferative and immunomodulatory activities of compounds isolated from Curcuma longa

    Science.gov (United States)

    Yue, Grace G. L.; Chan, Ben C. L.; Hon, Po-Ming; Lee, Mavis Y. H.; Fung, Kwok-Pui; Leung, Ping-Chung; Lau, Clara B. S.

    2010-01-01

    The rhizome of Curcuma longa (CL) has been commonly used in Asia as a potential candidate for the treatment of different diseases, including inflammatory disorders and cancers. The present study evaluated the anti-proliferative activities of the isolated compounds (3 curcuminoids and 2 turmerones) from CL, using human cancer cell lines HepG2, MCF-7 and MDA-MB-231. The immunomodulatory activities of turmerones (α and aromatic) isolated from CL were also examined using human peripheral blood mononuclear cells (PBMC). Our results showed that the curcuminoids (curcumin, demethoxycurcumin and bisdemethoxycurcumin) and α-turmerone significantly inhibited proliferation of cancer cells in dose-dependent manner. The IC50 values of these compounds in cancer cells ranged from 11.0–41.8 μg/ml. Alpha-turmerone induced MDA-MB-231 cells to undergo apoptosis, which was confirmed by annexin-V & propidium iodide staining, and DNA fragmentation assay. The caspase cascade was activated as shown by a significant decrease of procaspases-3, -8 and -9 in α-turmerone treated cells. Both α-turmerone and aromatic-turmerone showed stimulatory effects on PBMC proliferation and cytokine production. The anti-proliferative effect of α-turmerone and immunomodulatory activities of ar-turmerone were shown for the first time. The findings revealed the potential use of CL crude extract (containing curcuminoids and volatile oil including turmerones) as chemopreventive agent. PMID:20438793

  4. Antileishmanial, antimalarial and antimicrobial activities of the extract and isolated compounds from Austroplenckia populnea (Celastraceae).

    Science.gov (United States)

    Andrade, Sérgio F; da Silva Filho, Ademar A; de O Resende, Dimas; Silva, Márcio L A; Cunha, Wilson R; Nanayakkara, N P Dhammika; Bastos, Jairo Kenupp

    2008-01-01

    Austroplenckia populnea (Celastraceae), known as "marmelinho do campo", is used in Brazilian folk medicine as antimicrobial, anti-inflammatory, and antitumoural agent. The aim of the present work was to evaluate the antimicrobial, antileishmanial and antimalarial activities of the crude hydroalcoholic extract of A. populnea (CHE) and some of its isolated compounds. The phytochemical study of the CHE was carried out affording the isolation of methyl populnoate (1), populnoic acid (2), and stigmast-5-en-3-O-beta-(D-glucopyranoside) (3). This is the first time that the presence of compound 3 in A. populnea is reported. The results showed that the CHE presents antifungal and antibacterial activities, especially against Candida glabrata and Candida albicans, for which the CHE showed IC50 values of 0.7 microg mL(-1) and 5.5 microg mL(-1), respectively, while amphotericin B showed an IC50 value of 0.1 microg mL(-1) against both microorganisms. Compounds 1-3 were inactive against all tested microorganisms. In the antileishmanial activity test against Leishmania donovani, the CHE showed an IC50 value of 52 microg mL(-1), while compounds 2 and 3 displayed an IC50 value of 18 microg mL(-1) In the antimalarial assay against Plasmodium falciparum (D6 and W2 clones), it was observed that all evaluated samples were inactive. In order to compare the effect on the parasites with the toxicity to mammalian cells, the cytotoxicity activity of the isolated compounds was evaluated against Vero cells, showing that all evaluated samples exhibited no cytotoxicity at the maximum dose tested.

  5. Antibacterial activity of alkaloids produced by endophytic fungus Aspergillus sp. EJC08 isolated from medical plant Bauhinia guianensis.

    Science.gov (United States)

    Pinheiro, Eduardo Antonio A; Carvalho, Josiwander Miranda; dos Santos, Diellem Cristina P; Feitosa, André de Oliveira; Marinho, Patrícia Santana B; Guilhon, Giselle Maria Skelding Pinheiro; de Souza, Afonso Duarte L; da Silva, Felipe Moura A; Marinho, Andrey Moacir do R

    2013-01-01

    Bauhinia guianensis is a typical plant in the Amazon region belonging to the family Leguminosea, used by local populations for the treatment of infectious and renal diseases. Previous work on the plant B. guianensis led to the isolation of substances with anti-inflammatory and analgesic activities. Thus, compounds isolated from B. guianensis with antimicrobial activities had not been identified. Given that there is a possibility of biological activity reported for a given plant being found in the endophytic fungi, we decided to isolate endophytic fungi from B. guianensis and test their antimicrobial activities. The alkaloids known as fumigaclavine C and pseurotin A were isolated by column chromatography and identified by 1D and 2D NMR techniques and mass spectrometry. The alkaloids are first reported as broad-spectrum antibacterial agents with good activity.

  6. Isolation of Lactobacillus salivarius 1077 (NRRL B-50053) and characterization of its bacteriocin and spectra of antimicrobial activity

    Science.gov (United States)

    Lactobacillus salivarius 1077 (NRRL B-50053) was isolated from poultry intestinal materials after demonstrating in-vitro anti-Campylobacter jejuni activity. The isolate was then used for in-vitro fermentation. The protein content of the cell-free supernatant from the spent medium was precipitated ...

  7. Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity.

    Science.gov (United States)

    Moghadam, Morteza Shojaei; Albersmeier, Andreas; Winkler, Anika; Cimmino, Lorenzo; Rise, Kjersti; Hohmann-Marriott, Martin Frank; Kalinowski, Jörn; Rückert, Christian; Wentzel, Alexander; Lale, Rahmi

    2016-02-16

    Marine cold-temperature environments are an invaluable source of psychrophilic microbial life for new biodiscoveries. An Arctic marine bacterial strain collection was established consisting of 1448 individual isolates originating from biota, water and sediment samples taken at a various depth in the Barents Sea, North of mainland Norway, with an all year round seawater temperature of 4 °C. The entire collection was subjected to high-throughput screening for detection of extracellular laccase activity with guaiacol as a substrate. In total, 13 laccase-positive isolates were identified, all belonging to the Psychrobacter genus. From the most diverse four strains, based on 16S rRNA gene sequence analysis, all originating from the same Botryllus sp. colonial ascidian tunicate sample, genomic DNA was isolated and genome sequenced using a combined approach of whole genome shotgun and 8 kb mate-pair library sequencing on an Illumina MiSeq platform. The genomes were assembled and revealed genome sizes between 3.29 and 3.52 Mbp with an average G + C content of around 42%, with one to seven plasmids present in the four strains. Bioinformatics based genome mining was performed to describe the metabolic potential of these four strains and to identify gene candidates potentially responsible for the observed laccase-positive phenotype. Up to two different laccase-like multicopper oxidase (LMCO) encoding gene candidates were identified in each of the four strains. Heterologous expression of P11F6-LMCO and P11G5-LMCO2 in Escherichia coli BL21 (DE3) resulted in recombinant proteins exhibiting 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and guaiacol oxidizing activity. Thirteen Psychrobacter species with laccase-positive phenotype were isolated from a collection of Arctic marine bacteria. Four of the isolates were genome sequenced. The overall genome features were similar to other publicly available Psychrobacter genome sequences except for P11G5 harboring seven

  8. HYDROGEN PEROXIDE PRODUCTION ACTIVITY AND ADHESIVE PROPERTIES OF AEROCOCCI, ISOLATED IN WOMEN

    Directory of Open Access Journals (Sweden)

    Stepanskyi D.O.

    2017-06-01

    Full Text Available Introduction. Antagonistic activity of probiotic microorganisms against other species of bacteria is an important mechanism of their ecology and it is widely used in practice. This activity is inherent in many heme-deficient bacteria, which include aerococci, and can be composed of several components: the production of organic acids, antibiotics, lysozyme, hydrogen peroxide and others. Ability to produce hydrogen peroxide under aerobic conditions and in a state of relative anaerobiosis was established in aerococci. They were divided into strong and weak producers, depending on the amount of peroxides. Lack of data about peroxide-productive ability of aerococci, isolated from the lower genital tract of women, as well as a proven mechanism of hydrogen peroxide excretion in the oxidation of lactic acid, led to need in studying the aerococci hydrogen peroxide production level, to create autobacterial drugs, based on aerococci symbiont strains for sanitation of birth canal. Colonization resistance of the vaginal mucous and normal microflora value depends largely on the degree of adhesion of microbial cells to the mucosal surface. Along with numerous studies of lactobacilli adhesive properties to the vaginal epithelium, there are no data on the adsorption capacity of aerococci to the vaginal epithelial cells. Material and methods. 18 aerococci resident strains and 1 museum strain were explored in total. Presence and quantity of autosymbiont aerococci content in different parts of the birth tract (cervical canal, vagina, external genitalia skin (EGS and perineum was studied in 44 healthy women. Isolation and identification of aerococci from the women body was conducted by the method, taking into account growth on selective indicator medium, growth and biochemical activity in environments with selenium and tellurium salts, lactate oxidase and superoxide dismutase activity. Hydrogen peroxide was determined by iodometric method. Hydrogen peroxide

  9. Catalytic properties of IgMs with amylolytic activity isolated from patients with multiple sclerosis.

    Science.gov (United States)

    Ivanen, Dina R; Kulminskaya, Anna A; Shabalin, Konstantin A; Isaeva-Ivanova, Luydmila V; Ershova, Nadezhda A; Saveliev, Andrew N; Nevinsky, Gregory A; Neustroev, Kirill N

    2004-08-01

    Recently, amylolytic activity was detected in IgMs isolated from the sera of the patients with multiple sclerosis. All purified samples of IgM were electrophoretically homogenous and did not contain any co-purified a-amylase and a-glucosidase activities, in accordance with a set of criteria developed for abzymes. The amylolytic activity of abzymes was studied in the hydrolysis of p-nitrophenyl a-D-maltooligosaccharides with different degrees of polymerization from 1 to 8 by TLC and reverse-phase HPLC techniques. All IgM samples isolated from 54 patients with clinically definite multiple sclerosis demonstrated hydrolytic activity towards the above artificial substrates. The Michaelis constant values (Km) in the hydrolysis of p-nitrophenyl a-D-maltoheptaoside were in the range of 10 p-nitrophenyl or p-nitrophenyl a-D-glucosides, thus indicating the presence of an a-D-glucosidase activity. For a number of the investigated samples, specific amylolytic activity increased depending on the length of substrates (from p-nitrophenyl maltopentaoside to p-nitrophenyl maltohexaoside); for other IgMs, the opposite dependence was observed. All IgMs studied did not exhibit any other glycoside hydrolase activities toward p-nitrophenyl glycoside substrates. Abzyme fractions from different donors demonstrated catalytic heterogeneity in Michaelis-Menten parameters and different modes of action in the hydrolysis of p-nitrophenyl maltooligosaccharides. Enzymatic properties of the IgMs tested varied from human a-amylases. All investigated abzyme samples did not show transglycosylating ability.

  10. Characterization of fatty acid modifying enzyme activity in staphylococcal mastitis isolates and other bacteria

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    Lu Thea

    2012-06-01

    Full Text Available Abstract Background Fatty acid modifying enzyme (FAME has been shown to modify free fatty acids to alleviate their bactericidal effect by esterifying fatty acids to cholesterol or alcohols. Although it has been shown in previous studies that FAME is required for Staphylococcus aureus survival in skin abscesses, FAME is poorly studied compared to other virulence factors. FAME activity had also been detected in coagulase-negative staphylococci (CNS. However, FAME activity was only surveyed after a bacterial culture was grown for 24 h. Therefore if FAME activity was earlier in the growth phase, it would not have been detected by the assay and those strains would have been labeled as FAME negative. Results Fifty CNS bovine mastitis isolates and several S. aureus, Escherichia coli, and Streptococcus uberis strains were assayed for FAME activity over 24 h. FAME activity was detected in 54% of CNS and 80% S. aureus strains surveyed but none in E. coli or S. uberis. While some CNS strains produced FAME activity comparable to the lab strain of S. aureus, the pattern of FAME activity varied among strains and across species of staphylococci. All CNS that produced FAME activity also exhibited lipase activity. Lipase activity relative to colony forming units of these CNS decreased over the 24 h growth period. No relationship was observed between somatic cell count in the milk and FAME activity in CNS. Conclusions Some staphylococcal species surveyed produced FAME activity, but E. coli and S. uberis strains did not. All FAME producing CNS exhibited lipase activity which may indicate that both these enzymes work in concert to alter fatty acids in the bacterial environment.

  11. Characterization of CCN and IN activity of bacterial isolates collected in Atlanta, GA

    Science.gov (United States)

    Purdue, Sara; Waters, Samantha; Karthikeyan, Smruthi; Konstantinidis, Kostas; Nenes, Athanasios

    2016-04-01

    Characterization of CCN activity of bacteria, other than a few select types such as Pseudomonas syringae, is limited, especially when looked at in conjunction with corresponding IN activity. The link between these two points is especially important for bacteria as those that have high CCN activity are likely to form an aqueous phase required for immersion freezing. Given the high ice nucleation temperature of bacterial cells, especially in immersion mode, it is important to characterize the CCN and IN activity of many different bacterial strains. To this effect, we developed a droplet freezing assay (DFA) which consists of an aluminum cold plate, cooled by a continuous flow of an ethylene glycol-water mixture, in order to observe immersion freezing of the collected bacteria. Here, we present the initial results on the CCN and IN activities of bacterial samples we have collected in Atlanta, GA. Bacterial strains were collected and isolated from rainwater samples taken from different storms throughout the year. We then characterized the CCN activity of each strain using a DMT Continuous Flow Streamwise Thermal Gradient CCN Counter by exposing the aerosolized bacteria to supersaturations ranging from 0.05% to 0.6%. Additionally, using our new DFA, we characterized the IN activity of each bacterial strain at temperatures ranging from -20oC to 0oC. The combined CCN and IN activity gives us valuable information on how some uncharacterized bacteria contribute to warm and mixed-phase cloud formation in the atmosphere.

  12. Antimicrobial activity and safety evaluation of peptides isolated from the hemoglobin of chickens.

    Science.gov (United States)

    Hu, Fengjiao; Wu, Qiaoxing; Song, Shuang; She, Ruiping; Zhao, Yue; Yang, Yifei; Zhang, Meikun; Du, Fang; Soomro, Majid Hussain; Shi, Ruihan

    2016-12-05

    Hemoglobin is a rich source of biological peptides. As a byproduct and even wastewater of poultry-slaughtering facilities, chicken blood is one of the most abundant source of hemoglobin. In this study, the chicken hemoglobin antimicrobial peptides (CHAP) were isolated and the antimicrobial and bactericidal activities were tested by the agarose diffusion assay, minimum inhibitory concentration (MIC) analysis, minimal bactericidal concentration (MBC) analysis, and time-dependent inhibitory and bactericidal assays. The results demonstrated that CHAP had potent and rapid antimicrobial activity against 19 bacterial strains, including 9 multidrug-resistant bacterial strains. Bacterial biofilm and NaCl permeability assays, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were further performed to detect the mechanism of its antimicrobial effect. Additionally, CHAP showed low hemolytic activity, embryo toxicity, and high stability in different temperatures and animal plasma. CHAP may have great potential for expanding production and development value in animal medication, the breeding industry and environment protection.

  13. Molecular docking for thrombolytic activity of some isolated compounds from Clausena lansium.

    Directory of Open Access Journals (Sweden)

    Arkajyoti Paul

    2017-03-01

    Full Text Available Clausena lansium (Family- Rutaceae is commonly known as wampee, is found in fallow lands throughout Bangladesh. Our aim of the study to performed molecular docking studies to identify potential binding affinities of the phytocompounds from Clausena lansium, namely Clausemarin B, Clausenaline C, Clausenaline E, Murrayanine, vanillic acid and Xanthotoxol for searching of lead molecule for thrombolytic activity. A wide range of docking score found during molecular docking by Schrodinger. Clausemarin B , Clausenaline C , Clausenaline E, Murrayanine , vanillic acid and Xanthotoxol showed the docking score -6.926, -4.041, -4.889 , -4.356, -3.007 and -5.816 respectively. Among all the compounds Clausemarin B showed the best docking score. So, Clausemarin B is the best compounds for thrombolytic activity, as it possessed the best value in Molecular docking. Further in vivo investigation need to identify the thrombolytic activity of isolated compounds from Clausena lansium.

  14. Chemical Composition, Biological and Cytotoxic Activities of Plant Extracts and Compounds Isolated from Ferula lutea

    Directory of Open Access Journals (Sweden)

    Mansour Znati

    2014-02-01

    Full Text Available The present work describes the phytochemical study on Ferula lutea flowers. Total phenolics and flavonoids of the n-butanol and ethyl acetate extracts were quantified (phenolics [40.68–52.29 mg gallic acid equivalent/g of dry weight], flavonoids [12.38–14.72 mg quercitin/g dry weight]. Two diastereoisomers were isolated and identified using spectroscopic techniques (1D, 2D NMR and GC-MS. The extracts and diastereoisomers were tested for antioxidant, antiacetylcholinesterase, antimicrobial, antidiabectic, cytotoxic (leukemia cell line activities and allelopathic potentialities. The strongest antioxidant activity was obtained for the ethyl acetate extract (IC50 = 12.8 ± 1.29 µg/mL. The two extracts exhibited high antidiabetic activity (54.1 and 52.1% at 40 µg/mL.

  15. Anti-free radical activities of kaempferol isolated from Acacia nilotica (L.) Willd. Ex. Del.

    Science.gov (United States)

    Singh, Rajbir; Singh, Bikram; Singh, Sukhpreet; Kumar, Neeraj; Kumar, Subodh; Arora, Saroj

    2008-12-01

    In the present study the polyphenolic compound has been isolated from methanol extract of Acacia nilotica Willd. Ex. Del. which has been identified as kaempferol (AN-5) by NMR and mass spectroscopy. The antioxidant potential of the AN-5 was demonstrated in several in vitro assays: measuring the proton radical scavenging activity (DPPH scavenging assay), hydroxyl radical scavenging activity (deoxyribose degradation assay), metal chelating activity, reducing power and inhibition of lipid peroxidation. It was found that the effect of the compound AN-5 was strongly dose dependent up to the concentrations 1-50 microg/ml in DPPH assay and 1-100 microg/ml in deoxyribose degradation assay but did not show further change above the highest concentrations.

  16. Isolation and identification of a new homoisoflavan with potent antioxidant activity from Commelina Elegans

    International Nuclear Information System (INIS)

    Kabbash, A.; Yagi, A.; Ishizu, T.; Haraguchi, H.; Fujioka, T.; Moustafa, S.M.; El-Bassouny, Ashraf A.

    2008-01-01

    Potent antioxidative compounds have been isolated from the ethyl acetate extract of Commelina Elegans, Commelinaceae, using a repeated column chromatography. On the basis of spectral analysis, the compounds were identified as brazilin, brazilein and the new homoisoflavan-3, 7, 9, 3, 4-ol (4-4) dimer with X- and Y-conformers. The compounds inhibited both enzymatic lipid peroxidation using a rat liver microsomes and mitochondria and non-enzymatic reactions. The compounds completely protected the activities of the mitochondrial enzymes NADH- and succinate-cytochrome c reductase. Furthermore, radical scavenging activity on enzymatically and non-enzymatically generated superoxide anion was investigated. Electron spin resonance using spin trapping method suggested that the compounds have potent superoxide anion scavenging activities. (author)

  17. Characteristics and enhanced antioxidant activity of glycated Morchella esculenta protein isolate

    Directory of Open Access Journals (Sweden)

    Qiang ZHANG

    Full Text Available Abstract Morchella esculenta (L Pers. is a highly valued edible and medicinal fungus that remains underutilized. For this study, the effects of glycation treatment on antioxidant activity and characteristics of the M. esculenta protein isolate (MPI were investigated via the Maillard reaction. Conjugation between MPI and xylose was proven via UV-vis, FT-IR, intrinsic fluorescence analysis, and SDS-PAGE. Amino acid analysis revealed involvement of lysine, arginine and tyrosine in MPI, forming a covalent cross-link with xylose. Differential scanning calorimetry (DSC results showed that glycated MPI (MPIG possesses a more favorable thermal stability compared to native MPI (MPIN, heated MPI (MPIH and an unheated mixture of MPI and xylose (MPI-XM. MPIG exhibited significantly enhanced antioxidant activity compared to MPIN, MPIH, and MPI-XM. These results indicate MPIG can serve as a promising novel source of nutraceutical and functional ingredients that exert antioxidant activity.

  18. Tigecycline activity tested against 26,474 bloodstream infection isolates: a collection from 6 continents.

    Science.gov (United States)

    Sader, Helio S; Jones, Ronald N; Stilwell, Matthew G; Dowzicky, Michael J; Fritsche, Thomas R

    2005-07-01

    The activity of tigecycline (formerly GAR936), a novel glycylcycline, was tested against recent bloodstream infection (BSI) pathogen isolates from 6 continents. Frequency of clinical occurrence of these pathogens was determined and their antibiograms assessed using reference broth microdilution methods. A total of 26474 strains were tested for tigecycline susceptibility according to the Clinical and Laboratory Standards Institute (formerly the National Committee for Clinical Laboratory Standards) by the M7-A6 guidelines with interpretations from M100-S15 and the package insert. The rank order of pathogens was Staphylococcus aureus (33.1%), Escherichia coli (14.0%), coagulase-negative staphylococci (13.5%), Enterococcus spp. (12.3%), Klebsiella spp. (5.7%), Pseudomonas aeruginosa (4.2%), Enterobacter spp. (3.0%), beta-hemolytic streptococci (2.9%), Streptococcus pneumoniae (2.3%), and viridans group streptococci (1.4%). Tigecycline exhibited a broader spectrum of activity against BSI isolates when compared to ciprofloxacin, tetracycline, aminoglycosides, and many beta-lactams (imipenem). Tigecycline was highly active against most pathogens tested, including staphylococci (MIC(90), 0.5 microg/mL), enterococci (MIC90, 0.25 microg/mL), streptococci (MIC(90), < or =0.12 microg/mL), Escherichia coli (MIC90, 0.25 microg/mL), Klebsiella spp. (MIC90, 1 mmicrog/mL), and Enterobacter spp. (MIC(90), 2 mmicrog/mL), but showed limited inhibition of Pseudomonas aeruginosa (MIC90, 16 microg/mL) and indole-positive or indole-negative Proteae (MIC90, 4-8 microg/mL). In summary, tigecycline exhibited a wide spectrum of antimicrobial potency versus BSI isolates collected worldwide. Serious infections in nosocomial environments should benefit from tigecycline use among the investigational phase 3 agents focused toward resistant strains.

  19. Antibacterial activity of Achillea tenuifolia Lam. extract against standard bacteria and isolated strains

    Directory of Open Access Journals (Sweden)

    Sahar Omidpanah

    2016-12-01

    Full Text Available Researchers have been trying to develop new broad-spectrum antibiotics against the infectious diseases caused by bacteria, fungi, viruses, and parasites for many decades. Prolonged usage of the antibiotics has led to the emergence of drug resistance among bacteria; therefore, there is a tremendous need for novel antimicrobial agents from different sources such as plants which are used in traditional medicine. The aim of this study was to evaluate antibacterial effect of Achillea tenuifolia. The plant material was extracted by maceration method using methanol three times at room temperature. The extract was concentrated after removing the solvent by rotary evaporator and then lyophilized using freeze dryer. Inhibitory effect of the extract was examined against four standard bacteria strains and two isolated strains from diseased hen using disk diffusion method and microdilution method to evaluate their inhibition zone diameter (IZD and minimum inhibitory concentration (MIC, respectively. The results showed that the extract of the plant was active against standard strains including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis with IZDs of 10.3±0.5, 14±0.0, 12±0.0 and 11.6±0.5, respectively. However, growths of isolated strains were not inhibited in the presence of the extract. Although, the growths of isolated strains were not inhibited by the plant extract, the standard strains were moderately susceptible to the extract; among those P. aeroginosa was more sensible than other tested strains

  20. The antispasmodic activity of Buddleja scordioides and Buddleja perfoliata on isolated intestinal preparations.

    Science.gov (United States)

    Cortés, Alma Rosa; Delgadillo, Alba Jady; Hurtado, Marcela; Domínguez-Ramírez, Adriana Miriam; Medina, José Raúl; Aoki, Kazuko

    2006-06-01

    The antispasmodic activity of extracts from the aerial parts of Buddleja scordioides and Buddleja perfoliata (family: Scrophulariaceae) was studied on isolated tissue preparations from rabbit and guinea pig intestine. The chloroformic extract from the plants exhibited a significant relaxation on the spontaneous contraction of isolated rabbit jejunum at concentrations ranging from 1 to 400 microg/ml, and also caused an inhibitory effect on both K+ and Ca2+ induced contractions in the same tissue. The extracts at moderate doses (50 microg/ml) reduced 5-hydroxytryptamine (5-HT), acetylcholine and histamine induced contractions on isolated guinea pig ileum. Therefore, B. scordioides and B. perfoliata possess similar relaxant mechanism of action, in view of the fact that both inhibit K+ induce contraction and act through serotoninic, muscarinic and histaminic receptors. So, these data support the idea that the extracts may interfere either with calcium mobilization from intracellular stores, or with calcium interaction with regulatory proteins (e.g., calmodulin), or in other steps in the calcium signaling pathway. This leads us to suggest that the spasmolytic effect of both Buddleja species on smooth muscular contractility are due to the same or similar compounds occurring in these two species, which might be present in similar quantities.