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Sample records for acido docosahexaenoico dha

  1. Composicao quimica, perfil de acidos graxos e quantificacao dos acidos ƒ¿-linolenico, eicosapentaenoico e docosahexaenoico em visceras de tilapias (Oreochromis niloticus = Percentual composition, fatty acids and quantification of the LNA (Alfa-Linolenic, EPA (Eicosapentaenoic and DHA (Docosahexaenoic acids in visceras of Nile Tilapia (Oreochromis niloticus

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    Nilson Evelázio de Souza

    2005-01-01

    Full Text Available Foi avaliada a composição química de vísceras de tilápias (Oreochromis niloticus criadas em cativeiro Os teores de umidade, cinza, proteína bruta e lipídios totais foram de 64,4%; 1,3%; 6,3% e 18,0%, respectivamente, caracterizando alta concentração de lipídiostotais em relação a outros resíduos de peixes. Foram identificados 49 ácidos graxos, sendo majoritários os ácidos: oléico, (32,8%, seguido do palmítico, (19,9% e linoléico, (18,2%. As razões entre n-6/n-3 e ácidos poliinsaturados/saturados foram de 5,5 e 0,9, respectivamente. As quantificações dos ácidos graxos alfa-linolênico, eicosapentaenóico e docosahexaenóico, em mg/g de lipídios totais, foram de 10,4, 1,4 e 9,3, respectivamente. O elevado teor de lipídios totais das vísceras contribuiu significativamente para as quantidadesde ácidos graxos n-3. Todos os parâmetros analisados foram satisfatórios sob o ponto de vista nutricional e neste sentido as vísceras de tilápias poderão ser utilizadaa para alimentar peixes ou outros animais.The chemical composition was evaluated in visceras of tilapias raised in captivity. The moisture, ash, crude protein and total lipids contents were 64.4%; 1.3%; 6.3% and 18.0%, respectively, characterizing high total lipids concentration in relation other residues of fish. Forty nine fatty acids were detected, the major fatty acids were oleic (32.8%, palmitic (19.9% and linoleic-1 (18.2% and oleic (9.4%. The ratio n-6/n-3 and polyunsaturated/saturated fatty acids, showed the values 5.5 and 0.9, respectively. The quantifications of alfa-linolenic, eicosapentaenoic and docosahexaenoic acids (in mg/g of total lipids, were 10.4, 1.4 and 0.3, respectively. The higher contents of total lipids in visceras contributed significantly for amounts of n-3 fatty acids. All the parameters analyzed were shown nutritional value satisfactory in this sense visceras of tilapias can be used in the feed of fish and other animal.

  2. Supplementing female rats with DHA-lysophosphatidylcholine increases docosahexaenoic acid and acetylcholine contents in the brain and improves the memory and learning capabilities of the pups.

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    Rojas, I.

    2010-03-01

    Full Text Available Docosahexaenoic acid (DHA is supplied to the foetus and newborn through the mother from their own reserves and their diet. No consensus about the best form to supplement DHA has been established. We propose that DHAcontaining lysophosphatidylcholine (DHA-LPC, obtained from DHA-rich eggs may be a suitable form of DHA and choline (the precursor of acetylcholine supplementation. We evaluated the effectiveness of DHA-LPC to increase DHA and acetylcholine concentration in the brain of pups born from female rats supplemented with DHA-LPC before and during pregnancy. We also evaluated the effect of DHA supplementation on learning and memory capabilities of pups through the Skinner test for operant conditioning. Female Wistar rats received 40-day supplementation of DHA-LPC (8 mg DHA/kg b.w/daily., before and during pregnancy. After delivery, plasma, erythrocyte, liver, and adipose tissue DHA and plasma choline were analyzed. Brains from 60 day-old pups separated into frontal cortex, cerebellum, striatum, hippocampus, and occipital cortex, were assessed for DHA, acetylcholine, and acetylcholine transferase (CAT activity. Pups were subjected to the Skinner box test. DHA-LPC supplementation produces higher choline and liver DHA contents in the mother’s plasma and increases the pups’ DHA and acetylcholine in the cerebellum and hippocampus. CAT was not modified by supplementation. The Skinner test shows that pups born from DHA-LPC supplemented mothers exhibit better scores of learning and memory than the controls. Conclusion: DHA-LPC may be an adequate form for DHA supplementation during the perinatal period.El ácido docosahexaenoico (DHA que requiere el feto y el recién nacido lo aporta la madre desde sus reservas y la dieta, por lo cual se sugiere suplementar a la madre con DHA. No hay consenso sobre la mejor forma de suplementación. Proponemos que un lisofosfolípido que contiene DHA y colina (DHA-LPC obtenido de huevos con alto contenido de DHA es

  3. Docosahexaenoic acid (DHA, essentiality and requirements: why and how to provide supplementation

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    Nieto, Susana

    2006-06-01

    cido docosahexaenoico (C22:6, DHA es el ácido graso poliinsaturado de cadena larga de los fosfolípidos del cerebro más importante, siendo el 25% del total de los ácidos grasos. La mayor parte del DHA presente en el cerebro se incorpora durante el desarrollo de este, el que comienza a la 26ava semana gestacional, generando una alta demanda por el ácido graso hasta los dos años de edad. El DHA se requiere en el desarrollo cerebral durante la diferenciación neuronal y glial, y durante la mielinización y la sinaptogénesis neuronal El ácido graso debe ser incorporado a los lípidos del cerebro preformado ya que menos de un 5% de su precursor, el ácido alfa linolénico (LNA, se convierte a DHA. El feto humano tiene una capacidad muy limitada para sintetizar DHA a partir de LNA, por lo cual debe ser aportado por fuentes de origen materno. El DHA puede ser aportado por la madre a partir de tres fuentes principales; desde el tejido adiposo, cual es el principal reservorio del ácido graso, a partir de la biosíntesis desde el precursor LNA, la que ocurre principalmente en el hígado, o como ácido graso preformado proveniente de la dieta. Durante el período postnatal, el DHA es aportado por la madre al recién nacido a través de la leche. La nutrición occidental aporta baja cantidad de LNA y de DHA, y los Comités de Expertos en Nutrición sugieren que la madre debe recibir una suplementación con DHA durante el embarazo y la lactancia. Actualmente, la suplementación con DHA puede ser aportada a partir de diferentes fuentes; como DHA puro, como un etil éster, como aceite obtenido de microalgas, a partir de los fosfolípidos de la yema de huevo, o en la forma de un sn-2 monoacilglicerol. En esta revisión se discute sobre la evidencia que apoya la suplementación del recién nacido con DHA, la necesidad de la suplementación de la madre durante el embarazo y la lactancia, y sobre cuales son al presente las alternativas para proveer la suplementación con DHA.

  4. Docosahexaenoic acid (DHA, an essential fatty acid for the proper functioning of neuronal cells: their role in mood disorders

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    2009-06-01

    Full Text Available The brain and the nervous system are tissues with high contents of two polyunsaturated fatty acids: arachidonic acid (20:4, omega-6, AA and docosahexaenoic acid (22:6, omega-3, DHA. Despite their abundance in these tissues, AA and DHA cannot be re-synthesized in mammals. However, the concentration of these fatty acids can be modulated by dietary intake. AA and DHA must be provided by the diet as such (preformed or through the respective omega-6 and omega-3 precursors from vegetable origin. Linoleic acid, the precursor of AA is very abundant in the western diet and therefore the formation of AA from linoleic acid is not restrictive. On the other hand, alpha linolenic acid, the precursor of DHA is less available in our diet and preformed DHA is highly restrictive in some populations. During the last period of gestation and during the early post natal period, neurodevelopment occurs exceptionally quickly, and significant amounts of omega-6 and omega-3 polyunsaturated fatty acids, especially DHA, are critical to allow neurite outgrowth and the proper brain and retina development and function. In this review various functions of DHA in the nervous system, its metabolism into phospholipids, and its involvement in different neurological and mood disorders, such as Alzheimer’s disease, depression, and others are revised.

    El cerebro y el sistema nervioso son tejidos con un alto contenido de dos ácidos grasos poliinsaturados: el ácido araquidónico (20:4, omega-6, AA y el ácido docosahexaenoico (22:6, omega-3, DHA. A pesar de la abundancia de estos ácidos grasos en dichos tejidos los mamíferos no los pueden sintetizar de novo. Sin embargo, la concentración de estos ácidos grasos puede ser modificada por la dieta. El AA y el DHA pueden ser aportados por la dieta como tales (preformados o a partir de los respectivos precursores de origen vegetal. El ácido linoleico, precursor del AA es muy abundante en la dieta occidental, por lo cual la

  5. DHA Effects in Brain Development and Function

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    Lotte Lauritzen

    2016-01-01

    Full Text Available Docosahexaenoic acid (DHA is a structural constituent of membranes specifically in the central nervous system. Its accumulation in the fetal brain takes place mainly during the last trimester of pregnancy and continues at very high rates up to the end of the second year of life. Since the endogenous formation of DHA seems to be relatively low, DHA intake may contribute to optimal conditions for brain development. We performed a narrative review on research on the associations between DHA levels and brain development and function throughout the lifespan. Data from cell and animal studies justify the indication of DHA in relation to brain function for neuronal cell growth and differentiation as well as in relation to neuronal signaling. Most data from human studies concern the contribution of DHA to optimal visual acuity development. Accumulating data indicate that DHA may have effects on the brain in infancy, and recent studies indicate that the effect of DHA may depend on gender and genotype of genes involved in the endogenous synthesis of DHA. While DHA levels may affect early development, potential effects are also increasingly recognized during childhood and adult life, suggesting a role of DHA in cognitive decline and in relation to major psychiatric disorders.

  6. DHA Effects in Brain Development and Function.

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    Lauritzen, Lotte; Brambilla, Paolo; Mazzocchi, Alessandra; Harsløf, Laurine B S; Ciappolino, Valentina; Agostoni, Carlo

    2016-01-04

    Docosahexaenoic acid (DHA) is a structural constituent of membranes specifically in the central nervous system. Its accumulation in the fetal brain takes place mainly during the last trimester of pregnancy and continues at very high rates up to the end of the second year of life. Since the endogenous formation of DHA seems to be relatively low, DHA intake may contribute to optimal conditions for brain development. We performed a narrative review on research on the associations between DHA levels and brain development and function throughout the lifespan. Data from cell and animal studies justify the indication of DHA in relation to brain function for neuronal cell growth and differentiation as well as in relation to neuronal signaling. Most data from human studies concern the contribution of DHA to optimal visual acuity development. Accumulating data indicate that DHA may have effects on the brain in infancy, and recent studies indicate that the effect of DHA may depend on gender and genotype of genes involved in the endogenous synthesis of DHA. While DHA levels may affect early development, potential effects are also increasingly recognized during childhood and adult life, suggesting a role of DHA in cognitive decline and in relation to major psychiatric disorders.

  7. DHA involvement in neurotransmission process

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    Vancassel Sylvie

    2007-05-01

    Full Text Available The very high enrichment of the nervous system in the polyunsaturated fatty acids, arachidonic (AA, 20: 4n-6 and docosahexaenoic acids (DHA, 22: 6n-3, is dependant of the dietary availability of their respective precursors, linoleic (18: 2n-6 and_-linolenic acids (18: 3n-3. Inadequate amounts of DHA in brain membranes have been linked to a wide variety of abnormalities ranging from visual acuity and learning irregularities, to psychopathologies. However, the molecular mechanisms involved remain unknown. Several years ago, we hypothesized that a modification of DHA contents of neuronal membranes by dietary modulation could change the neurotransmission function and then underlie inappropriate behavioural response. We showed that, in parallel to a severe loss of brain DHA concomitant to a compensatory substitution by 22:5n-6, the dietary lack of α-linolenic acid during development induced important changes in the release of neurotransmitters (dopamine, serotonin, acetylcholine in cerebral areas specifically involved in learning, memory and reward processes. Data suggested alteration of presynaptic storage process and dysregulations of reciprocal functional interactions between monoaminergic and cholinergic pathways. Moreover, we showed that recovery of these neurochemical changes was possible when the deficient diet was switched to a diet balanced in n-3 and n-6 PUFA before weaning. The next step is to understand the mechanism involved. Particularly, we focus on the study of the metabolic cooperation between the endothelial cell, the astrocyte and the neuron which regulate synaptic transmission.These works could contribute to the understanding of the link between some neuropsychiatric disorders and the metabolism of n-3 PUFA, through their action on neurotransmission.

  8. Health benefits of docosahexaenoic acid (DHA)

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    Horrocks, L A; Yeo, Y K

    1999-09-01

    Docosahexaenoic acid (DHA) is essential for the growth and functional development of the brain in infants. DHA is also required for maintenance of normal brain function in adults. The inclusion of plentiful DHA in the diet improves learning ability, whereas deficiencies of DHA are associated with deficits in learning. DHA is taken up by the brain in preference to other fatty acids. The turnover of DHA in the brain is very fast, more so than is generally realized. The visual acuity of healthy, full-term, formula-fed infants is increased when their formula includes DHA. During the last 50 years, many infants have been fed formula diets lacking DHA and other omega-3 fatty acids. DHA deficiencies are associated with foetal alcohol syndrome, attention deficit hyperactivity disorder, cystic fibrosis, phenylketonuria, unipolar depression, aggressive hostility, and adrenoleukodystrophy. Decreases in DHA in the brain are associated with cognitive decline during aging and with onset of sporadic Alzheimer disease. The leading cause of death in western nations is cardiovascular disease. Epidemiological studies have shown a strong correlation between fish consumption and reduction in sudden death from myocardial infarction. The reduction is approximately 50% with 200 mg day(-1)of DHA from fish. DHA is the active component in fish. Not only does fish oil reduce triglycerides in the blood and decrease thrombosis, but it also prevents cardiac arrhythmias. The association of DHA deficiency with depression is the reason for the robust positive correlation between depression and myocardial infarction. Patients with cardiovascular disease or Type II diabetes are often advised to adopt a low-fat diet with a high proportion of carbohydrate. A study with women shows that this type of diet increases plasma triglycerides and the severity of Type II diabetes and coronary heart disease. DHA is present in fatty fish (salmon, tuna, mackerel) and mother's milk. DHA is present at low levels in

  9. DHA effects in brain development and function

    DEFF Research Database (Denmark)

    Lauritzen, Lotte; Brambilla, Paola; Mazzocchi, Allesandra;

    2016-01-01

    Docosahexaenoic acid (DHA) is a structural constituent of membranes specifically in the central nervous system. Its accumulation in the fetal brain takes place mainly during the last trimester of pregnancy and continues at very high rates up to the end of the second year of life. Since the endoge......Docosahexaenoic acid (DHA) is a structural constituent of membranes specifically in the central nervous system. Its accumulation in the fetal brain takes place mainly during the last trimester of pregnancy and continues at very high rates up to the end of the second year of life. Since...... the endogenous formation of DHA seems to be relatively low, DHA intake may contribute to optimal conditions for brain development. We performed a narrative review on research on the associations between DHA levels and brain development and function throughout the lifespan. Data from cell and animal studies...... justify the indication of DHA in relation to brain function for neuronal cell growth and differentiation as well as in relation to neuronal signaling. Most data from human studies concern the contribution of DHA to optimal visual acuity development. Accumulating data indicate that DHA may have effects...

  10. Dietary reference intakes for DHA and EPA.

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    Kris-Etherton, Penny M; Grieger, Jessica A; Etherton, Terry D

    2009-01-01

    Various organizations worldwide have made dietary recommendations for eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and fish intake that are primarily for coronary disease risk reduction and triglyceride (TG) lowering. Recommendations also have been made for DHA intake for pregnant women, infants, and vegetarians/vegans. A Dietary Reference Intake (DRI), specifically, an Adequate Intake (AI), has been set for alpha-linolenic acid (ALA) by the Institute of Medicine (IOM) of The National Academies. This amount is based on an intake that supports normal growth and neural development and results in no nutrient deficiency. Although there is no DRI for EPA and DHA, the National Academies have recommended that approximately 10% of the Acceptable Macronutrient Distribution Range (AMDR) for ALA can be consumed as EPA and/or DHA. This recommendation represents current mean intake for EPA and DHA in the United States ( approximately 100mg/day), which is much lower than what many groups worldwide are currently recommending. Global recommendations for long-chain omega-3 fatty acids underscore the pressing need to establish DRIs for DHA and EPA because DRIs are recognized as the "official" standard by which federal agencies issue dietary guidance or policy directives for the health and well-being of individuals in the United States and Canada. Because of the many health benefits of DHA and EPA, it is important and timely that the National Academies establish DRIs for the individual long-chain (20 carbons or greater) omega-3 fatty acids.

  11. Cross linkage studies with the membranes of the vesicular stomatitis virus using radioactive 4-acido and 5-acido palmitic acid

    International Nuclear Information System (INIS)

    In the study described here the spatial arrangement of lipids and proteins in the VS virus was investigated on the basis of the covalent cross linkage technique. The formation of such cross linkages is brought about by the action of photosensitive acidosubstituted lipids, which permit acido functions to be introduced into a membrane in a previously defined position. Subsequently, photolysis helps to trigger the generation of radioactive nitrenes that react with the proteins and lipids in their immediate vicinity in a direct and non-selective way. The findings revealed by this study have raised questions as to the possibility of lipid-protein and lipid-lipid interactions, which is also discussed. (orig./MG)

  12. Supplementation of a low dose of DHA or DHA plus AA does not prevent peripartum depressive symptoms in a small population based sample

    NARCIS (Netherlands)

    Doornbos, B.; van Goor, S. A.; Dijck-Brouwer, D. A. J.; Schaafsma, A.; Korf, J.; Muskiet, F. A. J.

    2009-01-01

    Background: The decrease of maternal docosahexaenoic (DHA) status during pregnancy has been associated with postpartum depression, especially in women with a low intake of DHA. Since the DHA intake in the Netherlands is low, we investigated whether supplementation of low doses of DHA or DHA plus ara

  13. Intrauterine, postpartum and adult relationships between arachidonic acid (AA) and docosahexaenoic acid (DHA).

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    Kuipers, Remko S; Luxwolda, Martine F; Janneke Dijck-Brouwer, D A; Muskiet, Frits A J

    2011-11-01

    Erythrocyte (RBC) fatty acid compositions from populations with stable dietary habits but large variations in RBC-arachidonic (AA) and RBC-docosahexaenoic acid (DHA) provided us with insight into relationships between DHA and AA. It also enabled us to estimate the maternal RBC-DHA (mRBC-DHA) status that corresponded with no decrease in mRBC-DHA during pregnancy, or in infant (i) RBC-DHA or mRBC-DHA during the first 3 months postpartum (DHA-equilibrium) while exclusively breastfeeding. At delivery, iRBC-AA is uniformly high and independent of mRBC-AA. Infants born to mothers with low RBC-DHA exhibit higher, but infants born to mothers with high RBC-DHA exhibit lower RBC-DHA than their mothers. This switch from 'biomagnification' into 'bioattenuation' occurs at 6g% mRBC-DHA. At 6g%, mRBC-DHA is stable throughout pregnancy, corresponds with postpartum infant DHA-equilibrium of 6 and 0.4g% DHA in mature milk, but results in postpartum depletion of mRBC-DHA to 5g%. Postpartum maternal DHA-equilibrium is reached at 8g% mRBC-DHA, corresponding with 1g% DHA in mature milk and 7g% iRBC-DHA at delivery that increases to 8g% during lactation. This 8g% RBC-DHA concurs with the lowest risks of cardiovascular and psychiatric diseases in adults. RBC-data from 1866 infants, males and (non-)pregnant females indicated AA vs. DHA synergism at low RBC-DHA, but antagonism at high RBC-DHA. These data, together with high intakes of AA and DHA from our Paleolithic diet, suggest that bioattenuation of DHA during pregnancy and postnatal antagonism between AA and DHA are the physiological standard for humans across the life cycle. PMID:21561751

  14. Breast milk docosahexaenoic acid (DHA) correlates with DHA status of malnourished infants

    NARCIS (Netherlands)

    Smit, EN; Oelen, EA; Seerat, E; Muskiet, FAJ; Boersma, ER

    2000-01-01

    Aim-To investigate whether low docosahexaenoic acid (22:6 omega 3; DHA) status of malnourished, mostly breast fed infants is a result of low omega 3 fatty acid intake via breast milk. Methods-Fatty acid composition of breast milk of eight Pakistani mothers, and of the erythrocytes of their malnouris

  15. Imaging plasma docosahexaenoic acid (dha incorporation into the brain in vivo, as a biomarker of brain DHA: Metabolism and neurotransmission

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    Rapoport Stanley I.

    2011-09-01

    Full Text Available Docosahexaenoic acid (DHA is critical for normal brain structure and function, and its brain concentration depends on dietary DHA content and hepatic conversion from its dietary derived n-3 precursor, a-linolenic acid (α-LNA. We developed an in vivo method in rats using quantitative autoradiography to image incorporation into brain of unesterified plasma DHA, and showed that the incorporation rate equals the rate of brain metabolic DHA consumption. Thus, quantitative imaging of DHA incorporation from plasma into brain can be used as a biomarker of brain DHA metabolism and neurotransmission. The method has been extended to humans with the use of positron emission tomography (PET. Furthermore, imaging in unanesthetized rats using DHA incorporation as a biomarker in response to N-methyl-D-aspartate (NMDA administration confirms that regional DHA signaling is independent of extracellular calcium, and likely mediated by a calcium-independent phospholipase A2 (iPLA2. Studies in mice in which iPLA2-VIA (β was knocked out confirmed that this enzyme is critical for baseline and muscarinic cholinergic signaling involving DHA.

  16. Impact of DHA on Metabolic Diseases from Womb to Tomb

    NARCIS (Netherlands)

    Arnoldussen, I.A.C.; Kiliaan, A.J.

    2014-01-01

    Long chain polyunsaturated fatty acids (LC-PUFAs) are important mediators in improving and maintaining human health over the total lifespan. One topic we especially focus on in this review is omega-3 LC-PUFA docosahexaenoic acid (DHA). Adequate DHA levels are essential during neurodevelopment and, i

  17. Intrauterine, postpartum and adult relationships between arachidonic acid (AA) and docosahexaenoic acid (DHA)

    NARCIS (Netherlands)

    Kuipers, Remko S.; Luxwolda, Martine F.; Dijck-Brouwer, D. A. Janneke; Muskiet, Frits A. J.

    2011-01-01

    Erythrocyte (RBC) fatty acid compositions from populations with stable dietary habits but large variations in RBC-arachidonic (AA) and RBC-docosahexaenoic acid (DHA) provided us with insight into relationships between DHA and AA. It also enabled us to estimate the maternal RBC-DHA (mRBC-DHA) status

  18. Restoring the DHA levels in the brains of Zellweger patients.

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    Martinez, M

    2001-01-01

    Patients with the Zellweger syndrome and its variants have very low levels of docosahexaenoic acid (DHA) in the brain, retina, and other tissues. Such a marked DHA deficiency could be related to the pathogenesis of peroxisomal disorders. Therefore, restoring the DHA levels in these patients can probably improve the clinical course of the disease. With this rationale, 20 patients with generalized peroxisomal disorders have been treated to date with DHA ethyl ester, at daily doses of 100-500 mg, for variable periods of time. Treatment has been always accompanied by a nutritious diet, normal for the age, in order to provide all the necessary nutrients and avoid a polyunsaturated fatty acid (PUFA) imbalance. The most constant improvement has been normalization of the DHA levels and liver function. Vision has improved in about half the patients and muscle tone has generally increased. Magnetic resonance imaging (MRI) examination revealed improvement of myelination in 9 patients. Significantly, the clinical improvement has been most marked in those patients who started the treatment before 6 mo of age. Biochemically, the plasma very long-chain fatty acids (VLCFA) 26:0 and 26:1n-9 decreased markedly despite the complete diet provided. In erythrocytes, the plasmalogen ratio 18: ODMA/18:0 increased in most cases, and sometimes even normalized. All these beneficial effects suggest that DHA deficiency plays a fundamental role in the pathogenesis of peroxisomal disease. Because DHA accretion is maximal during early brain development, it is essential to initiate the treatment as soon as possible. Otherwise, restoration of brain DHA levels and prevention of further damage will not be possible. PMID:11478386

  19. Lipidomic analysis of Arabidopsis seed genetically engineered to contain DHA

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    Xue-Rong eZhou

    2014-09-01

    Full Text Available Metabolic engineering of omega-3 long-chain (≥C20 polyunsaturated fatty acids (ω3 LC-PUFA in oilseeds has been one of the key metabolic engineering targets in recent years. By expressing a transgenic pathway for enhancing the synthesis of the ω3 LC-PUFA docosahexaenoic acid (DHA from endogenous -linolenic acid (ALA, we obtained the production of fish oil-like proportions of DHA in Arabidopsis seed oil. Liquid chromatography-mass spectrometry (LC-MS was used to characterize the triacylglycerol (TAG, diacylglycerol (DAG and phospholipid (PL lipid classes in the transgenic and wild type Arabidopsis seeds at both developing and mature stages. The analysis identified the appearance of several abundant DHA-containing phosphatidylcholine (PC, DAG and TAG molecular species in mature seeds. The relative abundances of PL, DAG and TAG species showed a preferred combination of LC-PUFA with ALA in the transgenic seeds, where LC-PUFA were esterified in positions usually occupied by 20:1ω9. Trace amounts of di-DHA PC and tri-DHA TAG were identified, and confirmed by high resolution MS/MS. Studying the lipidome in transgenic seeds provides insights into where DHA accumulated and composed with other fatty acids of neutral and phospholipids from the developing and mature seeds.

  20. The relation between the omega-3 index and arachidonic acid is bell shaped : Synergistic at low EPA plus DHA status and antagonistic at high EPA plus DHA status

    NARCIS (Netherlands)

    Luxwolda, Martine F.; Kuipers, Remko S.; Smit, Ella N.; Velzing-Aarts, Francien V.; Dijck-Brouwer, D. A. Janneke; Muskiet, Frits A. J.

    2011-01-01

    Introduction: The relation between docosahexaenoic (DHA) and eicosapentaenoic (EPA) vs. arachidonic acid (AA) seems characterized by both synergism and antagonism. Materials and methods: Investigate the relation between EPA + DHA and AA in populations with a wide range of EPA + DHA status and across

  1. Reducción de la biohidrogenación del ácido linoleico y alfa linolénico por la adición de diferentes proporciones de ácido eicosapentaenoico y docosahexaenoico

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    Julian Castillo

    2014-04-01

    Full Text Available Se evaluó el efecto de la adición de ácidodocosahexaenoicoe (DHA y eicosapentaenocio(EPA a una mezcla de ácidolinoleico (ALi y alfa-linolénico (ALn,sobre su biohidrogenación, en fluidoruminal in vitro. A 500 mg de kikuyo,se agregaron 18 μL de una mezcla deALi:ALn (75:25 (Control o una mezclade 14,9 μL ALi:ALn (75:25 con 3,1μL de una mezcla que contenía cuatrorelaciones de DHA:EPA (100:0, 75:25,25:75, 0:100. El kikuyo sin adición deácidos grasos (AG (Control negativo ocon adición de estos, fue incubado confluido ruminal por triplicado, durante 0,5 o 16 horas, en tubos de 50 mL. Loslípidos del material incubado fueron extraídos,metilados y analizados para AGpor CG-FID. Las concentraciones relativas(g/100 g AG de los AG incubados ylos principales productos de la isomerizacióny biohidrogenación se compararonmediante un modelo completamenteal azar, usando el PROC GLM de SAS.La concentración relativa de ALi y ALndisminuyó con el tiempo de incubación,pero no fue diferente entre tratamientoscon EPA y DHA a las 16 horas. La adiciónde DHA y EPA puros o en mezclasdisminuyó la concentración relativade C18:0 (P < 0,01 e incrementó la deC18:1 trans-11 (ATV (P < 0,01 y C18:2trans-11, cis-15 (P < 0,01 (ALC,mientras que los cambios en la concentraciónrelativa del C18:2 cis-9, trans-11no fueron claros. Los resultados sugieren que la acción inhibitoria del EPA y DHA ocurre en los pasos finales de la biohidrogenación del ALi y del ALn.

  2. Enhanced incorporation of dietary DHA into lymph phospholipids by altering its molecular carrier.

    Science.gov (United States)

    Subbaiah, Papasani V; Dammanahalli, Karigowda J; Yang, Peng; Bi, Jian; O'Donnell, J Michael

    2016-08-01

    Several previous studies indicated that for optimal uptake by the brain, docosahexaenoic acid (DHA) should be present as phospholipid in the plasma. However most of dietary DHA is absorbed as triacylglycerol (TAG) because it is released as free fatty acid during digestion of either TAG-DHA (fish oil) or sn-2-DHA phospholipid (krill oil), and subsequently incorporated into TAG of chylomicrons. We tested the hypothesis that the absorption of DHA as phospholipid can be increased if it is present in the sn-1 position of dietary phospholipid or in lysophosphatidylcholine (LPC), because it would escape the hydrolysis by pancreatic phospholipase A2. We infused micelle containing the DHA either as LPC or as free acid, into the duodenum of lymph cannulated rats, and analyzed the chylomicrons and HDL of the lymph for the DHA-containing lipids. The results show that while the total amount of DHA absorbed was comparable from the two types of micelle, the percentage of DHA recovered in lymph phospholipids was 5 times greater with LPC-DHA, compared to free DHA. Furthermore, the amount of DHA recovered in lymph HDL was increased by 2-fold when LPC-DHA micelle was infused. These results could potentially lead to a novel strategy to increase brain DHA levels through the diet. PMID:27178174

  3. One-generation reproductive toxicity study of DHA-rich oil in rats

    NARCIS (Netherlands)

    Blum, R.; Kiy, T.; Waalkens-Berendsen, I.; Wong, A.W.; Roberts, A.

    2007-01-01

    Polyunsaturated fatty acids, including docosahexaenoic acid (DHA), are natural constituents of the human diet. DHA-algal oil is produced through the use of the marine protist, Ulkenia sp. The reproductive toxicity of DHA-algal oil was assessed in a one-generation study. Rats were provided diets cont

  4. Effect of EPA and DHA Containing Glycerophospholipid Molecular Species on the Fluidity of Erythrocyte Cell Membranes

    OpenAIRE

    Nojima, Masahiro; Hosokawa, Masashi; Takahashi, Koretaro; HATANO, Mutsuo

    1994-01-01

    Fluorescence depolarization of erythrocytes was measured to evaluate the increase in fluidity of cell membrane due to treatment with soy phospholipids, hydrogenated soy phospholipids, icosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) containing phospholipids. When the erythrocytes were treated with EPA or DHA containing phosphatidylethanolamine or phosphatidylserine, the least fluorescence polarization was observed, followed by EPA or DHA containing phosphatidylcholine. All of the hy...

  5. A Correlation Study of DHA Dietary Intake and Plasma, Erythrocyte and Breast Milk DHA Concentrations in Lactating Women from Coastland, Lakeland, and Inland Areas of China.

    Science.gov (United States)

    Liu, Meng-Jiao; Li, Hong-Tian; Yu, Li-Xia; Xu, Gao-Sheng; Ge, Hua; Wang, Lin-Lin; Zhang, Ya-Li; Zhou, Yu-Bo; Li, You; Bai, Man-Xi; Liu, Jian-Meng

    2016-01-01

    We aimed to assess the correlation between docosahexaenoic acid (DHA) dietary intake and the plasma, erythrocyte and breast milk DHA concentrations in lactating women residing in the coastland, lakeland and inland areas of China. A total of 408 healthy lactating women (42 ± 7 days postpartum) were recruited from four hospitals located in Weihai (coastland), Yueyang (lakeland) and Baotou (inland) city. The categories of food containing DHA, the average amount consumed per time and the frequency of consumption in the past month were assessed by a tailored DHA food frequency questionnaire, the DHA Intake Evaluation Tool (DIET). DHA dietary intake (mg/day) was calculated according to the Chinese Food Composition Table (Version 2009). In addition, fasting venous blood (5 mL) and breast milk (10 mL) were collected from lactating women. DHA concentrations in plasma, erythrocyte and breast milk were measured using capillary gas chromatography, and were reported as absolute concentration (μg/mL) and relative concentration (weight percent of total fatty acids, wt. %). Spearman correlation coefficients were used to assess the correlation between intakes of DHA and its concentrations in biological specimens. The study showed that the breast milk, plasma and erythrocyte DHA concentrations were positively correlated with DHA dietary intake; corresponding correlation coefficients were 0.36, 0.36 and 0.24 for relative concentration and 0.33, 0.32, and 0.18 for absolute concentration (p food frequency questionnaire, DIET, is a valid tool for the assessment of DHA dietary intake. PMID:27213448

  6. MIKROENKAPSULASI FITOPLANKTON (Porphyridium cruentum) KAYA DHA DAN EPA

    OpenAIRE

    Mahmud, Roomy

    2015-01-01

    Abstrak. Dalam penelitian ini dilakukan kultivasi fitoplankton Porphyridium cruentum untuk memperoleh biomassa kemudian dilakukan pembuatan formula mikroenkapsul dengan berbagai variasi konsentrasi untuk melihat formula mikroenkapsul terbaik. Dari hasil analisis diperoleh DHA dan EPA berturut-turut sebesar 31,667 mg/g berat kering dan 69,688 mg/g berat kering. Hasil formulasi mikroenkapsul diperoleh mikroenkapsul F4 yang jadi formula terbaik.

  7. [CONSUMPTION OF OMEGA- 3 FATTY ACIDS AND DEPRESSIVE SYMPTOMS IN COLLEGE STUDENTS FROM SONORA, MEXICO].

    Science.gov (United States)

    Rubi Vargas, María; González Lomelí, Daniel; Terrazas Medina, Efraín A; Peralta Peña, Sandra L; Jordán Jinez, Ma Lourdes; Ruiz Paloalto, Ma Laura; Cupul Uicab, Lea A

    2015-10-01

    Introducción: estudios recientes sugieren que los niveles bajos de acidos grasos poliinsaturados omega-3 se relacionan con una mayor prevalencia de depresion; sin embargo, los resultados no son concluyentes. Objetivo: evaluar la asociacion entre el bajo consumo de acidos grasos omega-3 y la presencia de sintomas depresivos en jovenes universitarios del noroeste de Mexico. Métodos: se realizo un estudio transversal en una muestra de 706 universitarios de 18 a 24 anos de edad de ambos sexos. La presencia de sintomatologia depresiva se estimo con la escala de Depresion del Centro de Estudios Epidemiologicos (CES-D) empleando un punto de corte ≥ 24. El consumo semanal de acidos grasos alfalinolenico (ALA) y de eicosapentaenoico (EPA) mas docosahexaenoico (DHA) en mg/g de alimento se estimo por medio de un cuestionario de frecuencia de consumo de alimentos validado para la poblacion mexicana. La asociacion del consumo de omega-3 con la presencia de sintomas depresivos se evaluo con modelos de regresion logistica. Resultados: el 67% de los participantes fueron mujeres; en general, el 16,6% presentaron sintomatologia depresiva. El consumo bajo de ALA y EPA + DHA no se asocio con mayor prevalencia de sintomatologia depresiva antes y despues de ajustar por confusores. En aquellos casos con sintomatologia depresiva, los niveles ALA derivados solo de las nueces fueron significativamente menores. Conclusiones: en esta poblacion no se observo asociacion entre el bajo consumo de acidos grasos omega-3 y la sintomatologia depresiva. La posible asociacion entre el consumo de nueces y la sintomatologia depresiva requiere de mas estudios.

  8. The Relationship of Docosahexaenoic Acid (DHA with Learning and Behavior in Healthy Children: A Review

    Directory of Open Access Journals (Sweden)

    Norman Salem

    2013-07-01

    Full Text Available Childhood is a period of brain growth and maturation. The long chain omega-3 fatty acid, docosahexaenoic acid (DHA, is a major lipid in the brain recognized as essential for normal brain function. In animals, low brain DHA results in impaired learning and behavior. In infants, DHA is important for optimal visual and cognitive development. The usual intake of DHA among toddlers and children is low and some studies show improvements in cognition and behavior as the result of supplementation with polyunsaturated fatty acids including DHA. The purpose of this review was to identify and evaluate current knowledge regarding the relationship of DHA with measures of learning and behavior in healthy school-age children. A systematic search of the literature identified 15 relevant publications for review. The search found studies which were diverse in purpose and design and without consistent conclusions regarding the treatment effect of DHA intake or biomarker status on specific cognitive tests. However, studies of brain activity reported benefits of DHA supplementation and over half of the studies reported a favorable role for DHA or long chain omega-3 fatty acids in at least one area of cognition or behavior. Studies also suggested an important role for DHA in school performance.

  9. Supplementing female rats with DHA-lysophosphatidylcholine increases docosahexaenoic acid and acetylcholine contents in the brain and improves the memory and learning capabilities of the pups.

    OpenAIRE

    Rojas, I.; Zañartu, P.; Nieto, S.; Sanhueza, J.; Morgado, N.; A. Valenzuela

    2010-01-01

    Docosahexaenoic acid (DHA) is supplied to the foetus and newborn through the mother from their own reserves and their diet. No consensus about the best form to supplement DHA has been established. We propose that DHAcontaining lysophosphatidylcholine (DHA-LPC), obtained from DHA-rich eggs may be a suitable form of DHA and choline (the precursor of acetylcholine) supplementation. We evaluated the effectiveness of DHA-LPC to increase DHA and acetylcholine concentration in the brain of pups born...

  10. Different dietary omega-3 sources during pregnancy and DHA in the developing rat brain

    Directory of Open Access Journals (Sweden)

    Childs Caroline E.

    2011-09-01

    Full Text Available The essential n-3 fatty acid α-linolenic acid (ALA can be converted into eicosapentaenoic acid (EPA, docosapentaenoic acid (DPA and docosahexaenoic acid (DHA under the action of desaturase and elongase enzymes. Human studies have demonstrated that females convert a higher proportion of ALA into EPA and DHA than males. We have demonstrated that when fed upon an ALA rich diet, female rats have a significantly higher EPA content of plasma and liver lipids than males. When fetal tissues were collected, it was observed that pups from dams fed the ALA rich diet had a comparable brain DHA status to those from dams fed on a salmon-oil based diet, indicating that conversion of ALA to DHA during pregnancy was efficient, and that DHA accumulated in a tissue-specific manner. Similar efficacy of dietary ALA in women during pregnancy would mean that plant n-3 fatty acids would be useful alternatives to preformed EPA and DHA.

  11. Sources connues et potentielles de DHA pour les besoins de l’homme

    OpenAIRE

    Barnathan Gilles

    2007-01-01

    This paper focuses on the production of docosahexaenoic acid (DHA; 22:6 n-3), a major ω3 polyunsaturated fatty acid (PUFA) with applications in foods and pharmaceuticals. Fish oils are currently the main source of PUFA including EPA and DHA. Growing interest in PUFA properties in various fields coupled with their significance in health and dietary requirements has encouraged searching for more suitable sources of these compounds, specially DHA. Some methods in lipid extracting process now all...

  12. On the potential application of polar and temperate marine microalgae for EPA and DHA production

    OpenAIRE

    Boelen, Peter; van Dijk, Roechama; Sinninghe Damsté, Jaap S.; Rijpstra, W Irene C; Buma, Anita GJ

    2013-01-01

    Long chain polyunsaturated fatty acids (LC-PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are considered essential omega-3 fatty acids in human nutrition. In marine microalgae EPA and/or DHA are allegedly involved in the regulation of membrane fluidity and thylakoid membrane functioning. The cellular content of EPA and DHA may therefore be enhanced at low temperature and irradiance conditions. As a result, polar and cold temperate marine microalgal species might pot...

  13. DHA supplementation for late onset Stargardt disease: NAT-3 study

    Directory of Open Access Journals (Sweden)

    Giuseppe Querques

    2010-06-01

    Full Text Available Giuseppe Querques1, Pascale Benlian1, Bernard Chanu2, Nicolas Leveziel1, Gabriel Coscas1, Gisele Soubrane1, Eric H Souied11Department of Ophthalmology, University of Paris XII, Centre Hospitalier Intercommunal de Creteil, 2Department of Nutrition, University of Paris XII, Hopital Henry Mondor, Creteil, FranceBackground: We analyzed the effects of a docosahexaenoic acid (DHA supplementation in patients affected with late onset Stargardt disease (STGD.Methods: DHA (840 mg/day was given to 20 STGD patients for six months. A complete ophthalmologic examination, including best-corrected visual acuity (BCVA and multifocal electroretinogram (mfERG, was performed at inclusion day 0 (D0 and at month 6 (M6.Results: Overall, no statistical differences have been observed at M6 vs D0 as regards BCVA and mfERG (P > 0.05. Mild Improvement of BCVA and improvement of mfERG was noted in seven/40 eyes of four/20 patients. In the first patient, the peak of the a wave increased from 66 nV/deg² to 75.4 nV/deg² in the right eye (RE and 24.5 nV/deg² to 49.1 nV/deg² in the left eye (LE. The peak of the b wave improved from 122 nV/deg² to 157 nV/deg² in the RE, and 102 nV/deg² to 149 nV/deg² in the LE. In the second patient peaks of the a and b waves respectively increased from 11.8 nV/deg² to 72.1 nV/deg² and 53 nV/deg² to 185 nV/deg² in the RE. In the third patient the peak of the a wave increased from 37 nV/deg² to 43 nV/deg² in the RE, and from 31 nV/deg² to 45 nV/deg² in the LE; the peak of the b wave improved from 70 nV/deg² to 89 nV/deg² in the RE, and from 101 nV/deg² to 108 nV/deg² in the LE. In the fourth patient, the peak of the a wave increased from 39 nV/deg² to 42 nV/deg² in the RE, and from 40 nV/deg² to 43 nV/deg² in the LE; the peak of the b wave improved from 86 nV/deg² to 94 nV/deg² in the RE, and from 87 nV/deg² to 107 nV/deg² in the LE.Conclusion: DHA seems to influence some functional parameters in patients affected with

  14. Docosahexaenoic Acid (DHA: An Ancient Nutrient for the Modern Human Brain

    Directory of Open Access Journals (Sweden)

    Joanne Bradbury

    2011-05-01

    Full Text Available Modern humans have evolved with a staple source of preformed docosahexaenoic acid (DHA in the diet. An important turning point in human evolution was the discovery of high-quality, easily digested nutrients from coastal seafood and inland freshwater sources. Multi-generational exploitation of seafood by shore-based dwellers coincided with the rapid expansion of grey matter in the cerebral cortex, which characterizes the modern human brain. The DHA molecule has unique structural properties that appear to provide optimal conditions for a wide range of cell membrane functions. This has particular implications for grey matter, which is membrane-rich tissue. An important metabolic role for DHA has recently been identified as the precursor for resolvins and protectins. The rudimentary source of DHA is marine algae; therefore it is found concentrated in fish and marine oils. Unlike the photosynthetic cells in algae and higher plants, mammalian cells lack the specific enzymes required for the de novo synthesis of alpha-linolenic acid (ALA, the precursor for all omega-3 fatty acid syntheses. Endogenous synthesis of DHA from ALA in humans is much lower and more limited than previously assumed. The excessive consumption of omega-6 fatty acids in the modern Western diet further displaces DHA from membrane phospholipids. An emerging body of research is exploring a unique role for DHA in neurodevelopment and the prevention of neuropsychiatric and neurodegenerative disorders. DHA is increasingly being added back into the food supply as fish oil or algal oil supplementation.

  15. On the potential application of polar and temperate marine microalgae for EPA and DHA production

    NARCIS (Netherlands)

    Boelen, Peter; van Dijk, Roechama; Sinninghe Damsté, Jaap S; Rijpstra, W Irene C; Buma, Anita Gj

    2013-01-01

    Long chain polyunsaturated fatty acids (LC-PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are considered essential omega-3 fatty acids in human nutrition. In marine microalgae EPA and/or DHA are allegedly involved in the regulation of membrane fluidity and thylakoid membra

  16. Dietary intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in children - a workshop report

    NARCIS (Netherlands)

    Koletzko, B.; Uauy, R.; Palou, A.; Kok, F.J.; Hornstra, G.; Eilander, A.; Moretti, D.; Osendarp, S.J.M.; Zock, P.L.; Innis, S.

    2010-01-01

    There is controversy whether children should have a dietary supply of preformed long-chain polyunsaturated n-3 fatty acids EPA and DHA. The aims of the workshop were to review evidence for a possible benefit of a preformed EPA and/or DHA supply, of data required to set desirable intakes for children

  17. Screening on DHA-producing Antarctic bacteria N- 6 and its cultural conditions

    Institute of Scientific and Technical Information of China (English)

    Zhang Botao; Miao Jin-lai; Hui Hanxing; Wang Qing

    2006-01-01

    In Antarctic, the geography and climate differs from those in other places,and the bacteria there have adapted well to the environment there. Two hundred strains of bacteria were isolated from the sea ice in Antarctica. The bacteria were screened for DHA by means of GC, with fish oil as the standard. Seven strains containing DHA or EPA were obtained, among which the strain of No. N-6 was outstanding. And the component of DHA was identified by GC-MS. The relative content of DHA in N-6 was 8.72%, and total lipids in dry bacteria was 22.54%. The effects of some factors, including temperature, salinity and pH value, on the growth and DHAcontent of strain N-6 were studied. The results show that the DHA-content is relatively high when in low temperature and high pH, and the bacterium is psychrophilic, alkalophilic.

  18. DHA Depletion in Rat Brain Is Associated With Impairment on Spatial Learning and Memory

    Institute of Scientific and Technical Information of China (English)

    YING XIAO; LING WANG; RUO-JUN XU; ZHEN-YU CHEN

    2006-01-01

    Objective To examine the effect of docosahexaenoic acid (DHA) deficiency in brain on spatial learning and memory in rats. Methods Sprague Dawley rats were fed with an n-3 fatty acid deficient diet for two generations to induce DHA depletion in brain. DHA in seven brain regions was analyzed using the gas-liquid chromatography. Morris water maze (MWM) was employed as an assessing index of spatial learning and memory in the n-3 fatty acid deficient adult rats of second generation. Results Feeding an n-3 deficient diet for two generations depleted DHA differently by 39%-63% in the seven brain regions including cerebellum, medulla, hypothalamus, striatum, hippocampus, cortex and midbrain. The MWM test showed that the n-3 deficient rats took a longer time and swam a longer distance to find the escape platform than the n-3 Adq group. Conclusion The spatial learning and memory in adult rats are partially impaired by brain DHA depletion.

  19. Docosahexaenoic acid supplementation (DHA) and the return on investment for pregnancy outcomes.

    Science.gov (United States)

    Shireman, T I; Kerling, E H; Gajewski, B J; Colombo, J; Carlson, S E

    2016-08-01

    The Kansas University DHA Outcomes Study (KUDOS) found a significant reduction in early preterm births with a supplement of 600mg DHA per day compared to placebo. The objective of this analysis was to determine if hospital costs differed between groups. We applied a post-hoc cost analysis of the delivery hospitalization and all hospitalizations in the following year to 197 mother-infant dyads who delivered at Kansas University Hospital. Hospital cost saving of DHA supplementation amounted to $1678 per infant. Even after adjusting for the estimated cost of providing 600mg/d DHA for 26 weeks ($166.48) and a slightly higher maternal care cost ($26) in the DHA group, the net saving per dyad was $1484. Extrapolating this to the nearly 4 million US deliveries per year suggests universal supplementation with 600mg/d during the last 2 trimesters of pregnancy could save the US health care system up to USD 6 billion. PMID:27499448

  20. Metabolic engineering Camelina sativa with fish oil-like levels of DHA.

    Directory of Open Access Journals (Sweden)

    James R Petrie

    Full Text Available BACKGROUND: Omega-3 long-chain (≥C20 polyunsaturated fatty acids (ω3 LC-PUFA such as eicosapentaenoic acid (EPA and docosahexaenoic acid (DHA are critical for human health and development [corrected].. Numerous studies have indicated that deficiencies in these fatty acids can increase the risk or severity of cardiovascular, inflammatory and other diseases or disorders. EPA and DHA are predominantly sourced from marine fish although the primary producers are microalgae. Much work has been done to engineer a sustainable land-based source of EPA and DHA to reduce pressure on fish stocks in meeting future demand, with previous studies describing the production of fish oil-like levels of DHA in the model plant species, Arabidopsis thaliana. PRINCIPAL FINDINGS: In this study we describe the production of fish oil-like levels (>12% of DHA in the oilseed crop species Camelina sativa achieving a high ω3/ω6 ratio. The construct previously transformed in Arabidopsis as well as two modified construct versions designed to increase DHA production were used. DHA was found to be stable to at least the T5 generation and the EPA and DHA were found to be predominantly at the sn-1,3 positions of triacylglycerols. Transgenic and parental lines did not have different germination or seedling establishment rates. CONCLUSIONS: DHA can be produced at fish oil-like levels in industrially-relevant oilseed crop species using multi-gene construct designs which are stable over multiple generations. This study has implications for the future of sustainable EPA and DHA production from land-based sources.

  1. Interrelationships between maternal DHA in erythrocytes, milk and adipose tissue. Is 1wt% DHA the optimal human milk content? Data from four Tanzanian tribes differing in lifetime stable intakes of fish

    NARCIS (Netherlands)

    Luxwolda, Martine F.; Kuipers, Remko S.; Koops, Jan-Hein; Muller, Stefan; de Graaf, Deti; Dijck-Brouwer, Janneke; Muskiet, Frits A. J.

    2014-01-01

    Little is known about the interrelationships between maternal and infant erythrocyte-DHA, milk-DHA and maternal adipose tissue (AT)-DHA contents. We studied these relationships in four tribes in Tanzania (Maasai, Pare, Sengerema and Ukerewe) differing in their lifetime intakes of fish. Cross-section

  2. Contributions towards a specific DHA enrichment in the live food Brachionus plicatilis and Artemia sp.

    OpenAIRE

    Dhert, Ph.; Sorgeloos, P.; Devresse, B.

    1993-01-01

    This paper reports on enrichment techniques oriented towards a specific DHA accumulation in the live prey Brachionus plicatilis and Artemia sp. The product used in these experiments (DHA7) is an experimental emulsion with a DHA/EPA ratio 6.7:1. Rotifers enriched with this pure DHA7 emulsion contained up to 69 mg/g DW DHA and a DHA/EPA ratio of 1.6. The DHA/EPA ration could be increased to a much higher level by different enrichment techniques. Dilution of the DHA7 emulsion with coconut oil pr...

  3. Exercise and DHA prevent the negative effects of hypoxia on EEG and nerve conduction velocity.

    Science.gov (United States)

    Erken, Haydar Ali; Erken, Gülten; Colak, Rıdvan; Genç, Osman

    2013-12-01

    It is known that hypoxia has a negative effect on nervous system functions, but exercise and DHA (docosahexaenoic acid) have positive effect. In this study, it was investigated whether exercise and/or DHA can prevent the effects of hypoxia on EEG and nerve conduction velocity (NCV). 35 adult Wistar albino male rats were divided into five groups (n=7): control (C), hypoxia (H), hypoxia and exercise (HE), hypoxia and DHA (HD), and hypoxia and exercise and DHA (HED) groups. During the 28-day hypoxia exposure, the HE and HED groups of rats were exercised (0% incline, 30 m/min speed, 20 min/day, 5 days a week). In addition, DHA (36 mg/kg/day) was given by oral gavage to rats in the HD and HED groups. While EEG records were taken before and after the experimental period, NCV records were taken after the experimental period from anesthetized rats. Data were analyzed by paired t-test, one-way ANOVA, and post hoc Tukey test. In this study, it was shown that exposure to hypoxia decreased theta activity and NCV, but exercise and DHA reduced the delta activity, while theta, alpha, beta activities, and NCV were increased. These results have shown that the effects of hypoxia exposure on EEG and NCV can be prevented by exercise and/or DHA. PMID:24377343

  4. Sources connues et potentielles de DHA pour les besoins de l’homme

    Directory of Open Access Journals (Sweden)

    Barnathan Gilles

    2007-01-01

    Full Text Available This paper focuses on the production of docosahexaenoic acid (DHA; 22:6 n-3, a major ω3 polyunsaturated fatty acid (PUFA with applications in foods and pharmaceuticals. Fish oils are currently the main source of PUFA including EPA and DHA. Growing interest in PUFA properties in various fields coupled with their significance in health and dietary requirements has encouraged searching for more suitable sources of these compounds, specially DHA. Some methods in lipid extracting process now allow to get a better industrial use for fish by-products. An important objective is to find cultivated microbiological sources that delivered DHA but no EPA. Potentialities of marine bacteria, microalgae and marine protists are described. The dinoflagellate Crypthecodinium cohnii seems the most efficient microrganism for the large-scale production of DHA devoid of EPA. The marine protists Thraustochytrids offer promising possibilities for DHA and other major PUFA production. C. cohnii as well as Thraustochytrium and Schizochytrium are able to produce large biomass and lipid amounts, and DHA at levels up to 60%. The first results in the production of n-3 long-chain PUFA in transgenic plants are given.

  5. Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions

    OpenAIRE

    Zengxin Ma; Yanzhen Tan; Guzhen Cui; Yingang Feng; Qiu Cui; Xiaojin Song

    2015-01-01

    Aurantiochytrium is a promising docosahexaenoic acid (DHA) production candidate due to its fast growth rate and high proportions of lipid and DHA content. In this study, high-throughput RNA sequencing technology was employed to explore the acclimatization of this DHA producer under cold stress at the transcriptional level. The overall de novo assembly of the cDNA sequence data generated 29,783 unigenes, with an average length of 1,200 bp. In total, 13,245 unigenes were annotated in at least o...

  6. Dietary intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in children - a workshop report

    OpenAIRE

    Koletzko, B; Uauy, R; PALOU, A.; Kok, F. J.; Hornstra, G.; Eilander, A.; Moretti, D.; Osendarp, S.J.M.; Zock, P. L.; Innis, S

    2010-01-01

    There is controversy whether children should have a dietary supply of preformed long-chain polyunsaturated n-3 fatty acids EPA and DHA. The aims of the workshop were to review evidence for a possible benefit of a preformed EPA and/or DHA supply, of data required to set desirable intakes for children aged 2–12 years, and of research priorities. The authors concluded that EPA and DHA intakes per kg body weight may often be low in 2- to 12-year-old children, relative to intakes per kg body weigh...

  7. Omega-3 fatty acids for nutrition and medicine: considering microalgae oil as a vegetarian source of EPA and DHA.

    Science.gov (United States)

    Doughman, Scott D; Krupanidhi, Srirama; Sanjeevi, Carani B

    2007-08-01

    Long-chain EPA/DHA omega-3 fatty acid supplementation can be co-preventative and co-therapeutic. Current research suggests increasing accumulated long chain omega-3s for health benefits and as natural medicine in several major diseases. But many believe plant omega-3 sources are nutritionally and therapeutically equivalent to the EPA/DHA omega-3 in fish oil. Although healthy, precursor ALA bio-conversion to EPA is inefficient and production of DHA is nearly absent, limiting the protective value of ALA supplementation from flax-oil, for example. Along with pollutants certain fish acquire high levels of EPA/DHA as predatory species. However, the origin of EPA/DHA in aquatic ecosystems is algae. Certain microalgae produce high levels of EPA or DHA. Now, organically produced DHA-rich microalgae oil is available. Clinical trials with DHA-rich oil indicate comparable efficacies to fish oil for protection from cardiovascular risk factors by lowering plasma triglycerides and oxidative stress. This review discusses 1) omega-3 fatty acids in nutrition and medicine; 2) omega-3s in physiology and gene regulation; 3) possible protective mechanisms of EPA/DHA in major diseases such as coronary heart disease, atherosclerosis, cancer and type 2 diabetes; 4) EPA and DHA requirements considering fish oil safety; and 5) microalgae EPA and DHA-rich oils and recent clinical results. PMID:18220672

  8. Bax translocation into mitochondria during dihydroartemisinin(DHA)-induced apoptosis in human lung adenocarcinoma cells

    Science.gov (United States)

    Lu, Ying-ying; Chen, Tong-sheng; Qu, Jun-Le

    2009-02-01

    Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, isolated from the traditional Chinese herb Artemisia annua, has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathways. However, the molecular mechanisms are not well understood. This study was investigated in human lung adenocarconoma ASTC-a-1 cell line and aimed to determine whether the apoptotic process was mediated by Bax activation and translocation during DHA-induced apoptosis. In this study, DHA induced a time-dependent apoptotic cell death, which was assayed by Cell Counting Kit (CCK-8) and Hoechst 33258 staining. Detection of Bax aggregation and translocation to mitochondria was observed in living cells which were co-transfected with GFP-Bax and Dsred-mito plasmid using confocal fluorescence microscope technique. Overall, these results demonstrated that Bax activation and translocation to mitochondria occurred during DHA-induced apoptosis.

  9. Role of DHA in aging-related changes in mouse brain synaptic plasma membrane proteome.

    Science.gov (United States)

    Sidhu, Vishaldeep K; Huang, Bill X; Desai, Abhishek; Kevala, Karl; Kim, Hee-Yong

    2016-05-01

    Aging has been related to diminished cognitive function, which could be a result of ineffective synaptic function. We have previously shown that synaptic plasma membrane proteins supporting synaptic integrity and neurotransmission were downregulated in docosahexaenoic acid (DHA)-deprived brains, suggesting an important role of DHA in synaptic function. In this study, we demonstrate aging-induced synaptic proteome changes and DHA-dependent mitigation of such changes using mass spectrometry-based protein quantitation combined with western blot or messenger RNA analysis. We found significant reduction of 15 synaptic plasma membrane proteins in aging brains including fodrin-α, synaptopodin, postsynaptic density protein 95, synaptic vesicle glycoprotein 2B, synaptosomal-associated protein 25, synaptosomal-associated protein-α, N-methyl-D-aspartate receptor subunit epsilon-2 precursor, AMPA2, AP2, VGluT1, munc18-1, dynamin-1, vesicle-associated membrane protein 2, rab3A, and EAAT1, most of which are involved in synaptic transmission. Notably, the first 9 proteins were further reduced when brain DHA was depleted by diet, indicating that DHA plays an important role in sustaining these synaptic proteins downregulated during aging. Reduction of 2 of these proteins was reversed by raising the brain DHA level by supplementing aged animals with an omega-3 fatty acid sufficient diet for 2 months. The recognition memory compromised in DHA-depleted animals was also improved. Our results suggest a potential role of DHA in alleviating aging-associated cognitive decline by offsetting the loss of neurotransmission-regulating synaptic proteins involved in synaptic function. PMID:27103520

  10. Omega-3 Fatty Acids EPA and DHA: Health Benefits Throughout Life12

    OpenAIRE

    Swanson, Danielle; Block, Robert; Mousa, Shaker A

    2012-01-01

    Omega-3 [(n-3)] fatty acids have been linked to healthy aging throughout life. Recently, fish-derived omega-3 fatty acids EPA and DHA have been associated with fetal development, cardiovascular function, and Alzheimer’s disease. However, because our bodies do not efficiently produce some omega-3 fatty acids from marine sources, it is necessary to obtain adequate amounts through fish and fish-oil products. Studies have shown that EPA and DHA are important for proper fetal development, includin...

  11. Impact of Genotype on EPA and DHA Status and Responsiveness to Increased Intakes

    OpenAIRE

    Anne Marie Minihane

    2016-01-01

    At a population level, cardioprotective and cognitive actions of the fish oil (FO) derived long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been extensively demonstrated. In addition to dietary intake, which is limited for many individuals, EPA and DHA status is dependent on the efficiency of their biosynthesis from α-linolenic acid. Gender and common gene variants have been identified as influencing the rate-limiting de...

  12. Recent developments in the commercial production of DHA and EPA rich oils from micro-algae

    OpenAIRE

    Winwood Robert J.

    2013-01-01

    The regular intake of marine omega 3’s DHA and EPA has been scientifically established as providing a wide range of health benefits. This paper reviews recent developments in the commercial production of DHA and EPA rich oils from micro-algae. The selection of suitable micro-algae species is discussed. The complexities of producing algal oil rich in marine omega 3’s is examined in terms of both upstream and downstream production.

  13. Modulatory Effects of EPA and DHA on Proliferation and Apoptosis of Pancreatic Cancer Cells

    Institute of Scientific and Technical Information of China (English)

    ZHANG Weikang; LONG Yueping; ZHANG Jinghui; WANG Chunyou

    2007-01-01

    In order to investigate the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the proliferation, apoptosis of pancreatic cancer cell line SW1990 cells and the ex-pression of cyclin E mRNA, the SW1990 cells were treated with different concentrations of EPA or DHA (20, 40, 60 μg/mL) for 0, 12, 24, 36 and 48 h respectively. By using MTT method, the inhibi-tory effects of EPA or DHA on the cell growth were assayed. Real time PCR was used to detect the expression changes of cyclin E mRNA after the SW1990 cells were treated with 40 μg/mL EPA or DHA for different time. Flow eytometry was used to test the changes of apoptostic rate in the SW1990 cells treated with different concentrations of EPA or DHA for 24 h. The results showed that EPA and DHA could inhibit the growth of SW1990 cells in a time- and concentration-dependent manner (P<0.01). EPA or DHA could also significantly inhibit the expression of cyclin E mRNA in a time-dependent manner (P<0.05). EPA or DHA could induce the apoptosis of SW1990 cells in a concentration-dependent manner (P<0.01). It was concluded that ω-3 fatty acid could inhibit the pro- liferation of pancreatic cancer cell line SW1990 cells and promote their apoptosis. The down-regulation of the cyclin E expression by ω-3 fatty acid might be one of the mechanisms for its anti-tumor effect on pancreatic cancer.

  14. Comparative analysis of EPA and DHA in fish oil nutritional capsules by GC-MS

    OpenAIRE

    Yi, Tao; Li, Shuk-Man; Fan, Jia-Yi; Fan, Lan-Lan; Zhang, Zhi-feng; Luo, Pei; Zhang, Xiao-Jun; Wang, Jian-Gang; Zhu, Lin; Zhao, Zhong-zhen; Chen, Hu-Biao

    2014-01-01

    Background Fish oil is a popular nutritional product consumed in Hong Kong. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are the two main bioactive components responsible for the health benefits of fish oil. Market survey in Hong Kong demonstrated that various fish oil capsules with different origins and prices are sold simultaneously. However, these capsules are labelled with same ingredient levels, namely EPA 180 mg/g and DHA 120 mg/g. This situation makes the consumers very c...

  15. Different dietary omega-3 sources during pregnancy and DHA in the developing rat brain

    OpenAIRE

    Childs Caroline E.; Fear Alison L.; Hoile Samuel P.; Calder Philip C

    2011-01-01

    The essential n-3 fatty acid α-linolenic acid (ALA) can be converted into eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) under the action of desaturase and elongase enzymes. Human studies have demonstrated that females convert a higher proportion of ALA into EPA and DHA than males. We have demonstrated that when fed upon an ALA rich diet, female rats have a significantly higher EPA content of plasma and liver lipids than males. When fetal tissues were ...

  16. Food Sources of EPA and DHA in the Diets of American Children, NHANES 2003-2010

    OpenAIRE

    Kranz, Sibylle; Huss, Lyndsey R.; Dobbs-Oates, Jennifer

    2015-01-01

    Objective Dietary eicosapentaenoicacid (EPA) and docosahexaenoic acid (DHA) are found in the highest concentrations in fish and seafood. As important nutrients for brain and eye development and function, their consumption levels are of public health interest, especially in children. This study was conducted toexamine children’sreported consumption of fish and shellfish as well as EPA and DHA intake.Methods Secondary analysis of dietary intake (24-hour recall) and Food Frequency Questionnaire ...

  17. N-3 polyunsaturated fatty acid DHA during IVM affected oocyte developmental competence in cattle.

    Science.gov (United States)

    Oseikria, Mouhamad; Elis, Sébastien; Maillard, Virginie; Corbin, Emilie; Uzbekova, Svetlana

    2016-06-01

    The positive effect of n-3 polyunsaturated fatty acids (FAs) on fertility in ruminants seems to be partly mediated through direct effects on the oocyte developmental potential. We aimed to investigate whether supplementation with physiological levels of docosahexaenoic acid (DHA, C22:6 n-3 polyunsaturated fatty acids) during IVM has an effect on oocyte maturation and in vitro embryo development in cattle. We reported that DHA (0, 1, 10, or 100 μM) had no effect on oocyte viability or maturation rate after 22-hour IVM. Incubation of oocyte-cumulus complexes with 1-μM DHA during IVM significantly increased (P reaction, negative effects of 100-μM DHA were associated with significant increase of progesterone synthesis by oocyte-cumulus complexes, a three-fold increase in expression level of FA transporter CD36 and a two-fold decrease of FA synthase FASN genes in cumulus cells (CCs) of corresponding oocytes. Docosahexaenoic acid at 1 and 10 μM had no effect on expression of those and other key lipid metabolism-related genes in CC. In conclusion, administration of a low physiological dose of DHA (1 μM) during IVM may have beneficial effects on oocyte developmental competence in vitro without affecting lipid metabolism gene expression in surrounding CCs, contrarily to 100 μM DHA which diminished oocyte quality associated with perturbation of lipid and steroid metabolism in CC. PMID:26898414

  18. Dihydroartemisinin (DHA induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells

    Directory of Open Access Journals (Sweden)

    Sun Lei

    2009-02-01

    Full Text Available Abstract Background Dihydroartemisinin (DHA, a semi-synthetic derivative of artemisinin, isolated from the traditional Chinese herb Artemisia annua, is recommended as the first-line anti-malarial drug with low toxicity. DHA has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathways, although the molecular mechanisms are not well understood. Methods In this study, cell counting kit (CCK-8 assay was employed to evaluate the survival of DHA-treated ASTC-a-1 cells. The induction of apoptosis was detected by Hoechst 33258 and PI staining as well as flow cytometry analysis. Collapse of mitochondrial transmembrane potential (ΔΨm was measured by dynamic detection under a laser scanning confocal microscope and flow cytometry analysis using Rhodamine123. Caspase-3 activities measured with or without Z-VAD-fmk (a broad spectrum caspase inhibitor pretreatment by FRET techniques, caspase-3 activity measurement, and western blotting analysis. Results Our results indicated that DHA induced apoptotic cell death in a dose- and time-dependent manner, which was accompanied by mitochondrial morphology changes, the loss of ΔΨm and the activation of caspase-3. Conclusion These results show for the first time that DHA can inhibit proliferation and induce apoptosis via caspase-3-dependent mitochondrial death pathway in ASTC-a-1 cells. Our work may provide evidence for further studies of DHA as a possible anticancer drug in the clinical treatment of lung adenocarcinoma.

  19. Supplementing female rats with DHA-lysophosphatidylcholine increases docosahexaenoic acid and acetylcholine contents in the brain and improves the memory and learning capabilities of the pups

    Energy Technology Data Exchange (ETDEWEB)

    Valenzuela, A.; Nieto, S.; Sanhueza, J.; Morgado, N.; Rojas, I.; Zanartu, P.

    2010-07-01

    Docosahexaenoic acid (Dha) is supplied to the foetus and newborn through the mother from their own reserves and their diet. No consensus about the best form to supplement DHA has been established. We propose that DHA containing lysophosphatidylcholine (DHA-LPC), obtained from DHA-rich eggs may be a suitable form of DHA and choline (the precursor of acetylcholine) supplementation. We evaluated the effectiveness of DHA-LPC to increase DHA and acetylcholine concentration in the brain of pups born from female rats supplemented with DHA-LPC before and during pregnancy. We also evaluated the effect of DHA supplementation on learning and memory capabilities of pups through the Skinner test for operant conditioning. Female Wistar rats received 40-day supplementation of DHA-LPC (8 mg DHA/kg b.w/daily.), before and during pregnancy. After delivery, plasma, erythrocyte, liver, and adipose tissue DHA and plasma choline were analyzed. Brains from 60 day-old pups separated into frontal cortex, cerebellum, striatum, hippocampus, and occipital cortex, were assessed for DHA, acetylcholine, and acetylcholine transferase (CAT) activity. Pups were subjected to the Skinner box test. DHA-LPC supplementation produces higher choline and liver DHA contents in the mothers plasma and increases the pups DHA and acetylcholine in the cerebellum and hippocampus. CAT was not modified by supplementation. The Skinner test shows that pups born from DHA-LPC supplemented mothers exhibit better scores of learning and memory than the controls. Conclusion: DHA-LPC may be an adequate form for DHA supplementation during the perinatal period. (Author) 66 refs.

  20. Reduction in DHA transport to the brain of mice expressing human APOE4 compared to APOE2.

    Science.gov (United States)

    Vandal, Milène; Alata, Wael; Tremblay, Cyntia; Rioux-Perreault, Christine; Salem, Norman; Calon, Frédéric; Plourde, Mélanie

    2014-05-01

    Benefits on cognition from docosahexaenoic acid (DHA, 22 : 6 n-3) intake are absent in humans carrying apolipoprotein E ε4 allele (APOE4), the most important genetic risk factor for Alzheimer's disease (AD). To test the hypothesis that carrying APOE4 impairs DHA distribution, we evaluated plasma and brain fatty acid profiles and uptake of [(14) C]-DHA using in situ cerebral perfusion through the blood-brain barrier in 4- and 13-month-old male and female APOE-targeted replacement mice (APOE2, APOE3, and APOE4), fed with a DHA-depleted diet. Cortical and plasma DHA were 9% lower and 34% higher in APOE4 compared to APOE2 mice, respectively. Brain uptake of [(14) C]-DHA was 24% lower in APOE4 versus APOE2 mice. A significant relationship was established between DHA and apoE concentrations in the cortex of mice (r(2) = 0.21) and AD patients (r(2) = 0.32). Altogether, our results suggest that lower brain uptake of DHA in APOE4 than in APOE2 mice may limit the accumulation of DHA in cerebral tissues. These data provide a mechanistic explanation for the lack of benefit of DHA in APOE4 carriers on cognitive function and the risk of AD. Using human APOE2, 3, and 4 isoform-specific transgenic mice, we found a lower brain uptake of docosahexaenoic acid (DHA) in APOE4 than in APOE2 mice that may limit the biodistribution of DHA in cerebral tissues. These data provide a mechanistic explanation for the lack of benefit of DHA in APOE4 carriers on cognitive function and the risk of Alzheimer's disease (AD).

  1. Chemopreventive and renal protective effects for docosahexaenoic acid (DHA: implications of CRP and lipid peroxides

    Directory of Open Access Journals (Sweden)

    Darweish MM

    2009-04-01

    Full Text Available Abstract Background The fish oil-derived ω-3 fatty acids, like docosahexanoic (DHA, claim a plethora of health benefits. We currently evaluated the antitumor effects of DHA, alone or in combination with cisplatin (CP in the EAC solid tumor mice model, and monitored concomitant changes in serum levels of C-reactive protein (CRP, lipid peroxidation (measured as malondialdehyde; MDA and leukocytic count (LC. Further, we verified the capacity of DHA to ameliorate the lethal, CP-induced nephrotoxicity in rats and the molecular mechanisms involved therein. Results EAC-bearing mice exhibited markedly elevated LC (2-fold, CRP (11-fold and MDA levels (2.7-fold. DHA (125, 250 mg/kg elicited significant, dose-dependent reductions in tumor size (38%, 79%; respectively, as well as in LC, CRP and MDA levels. These effects for CP were appreciably lower than those of DHA (250 mg/kg. Interestingly, DHA (125 mg/kg markedly enhanced the chemopreventive effects of CP and boosted its ability to reduce serum CRP and MDA levels. Correlation studies revealed a high degree of positive association between tumor growth and each of CRP (r = 0.85 and leukocytosis (r = 0.89, thus attesting to a diagnostic/prognostic role for CRP. On the other hand, a single CP dose (10 mg/kg induced nephrotoxicity in rats that was evidenced by proteinuria, deterioration of glomerular filtration rate (GFR, -4-fold, a rise in serum creatinine/urea levels (2–5-fold after 4 days, and globally-induced animal fatalities after 7 days. Kidney-homogenates from CP-treated rats displayed significantly elevated MDA- and TNF-α-, but reduced GSH-, levels. Rats treated with DHA (250 mg/kg, but not 125 mg/kg survived the lethal effects of CP, and showed a significant recovery of GFR; while their homogenates had markedly-reduced MDA- and TNF-α-, but -increased GSH-levels. Significant association was detected between creatinine level and those of MDA (r = 0.81, TNF-α r = 0.92 and GSH (r = -0

  2. Application of Microalgal DHA in Cake%微藻DHA在蛋糕中的应用

    Institute of Scientific and Technical Information of China (English)

    彭云; 李汴生; 林应胜; 黄巍峰; 张影霞

    2012-01-01

    Sensory evaluation of the cake added with micro-algae DHA and determination of the DHA, peroxide value and acid value, etc, were made in this paper. The results showed that adding micro-algae DHA did not cause significant changes in the smell and taste of the cake. In the test of the micro-algae DHA additives (0.10 mg/g~1.00 mg/g finished product) inside, the average loss rate of the DHA in cake after baking is 12.28% and DHA loss also changes very little in room temperature placed after 2 d. No significant changes in the peroxide value and acid value of the cake were found in the process of production and placement. The cake's main ingredients showed little influence on the stability of the micro-algae DHA. The micro-algae DHA of the test can be used in the production of cake, and new functional food containing DHA will be developed.%通过在蛋糕配料中加入微藻DHA,评价蛋糕的感官和测定蛋糕中的DHA含量、过氧化值和酸价等,探讨微藻DHA在蛋糕生产中应用的可行性.结果表明,添加微藻DHA不会对蛋糕的气味、口感产生明显的影响;在试验的微藻DHA添加量(0.10mg/g~1.00 mg/g成品)内,蛋糕在烘烤后DHA的损失率平均在12.28%,室温放置2d后DHA的损失率也变化很小;在此生产和放置过程中蛋糕的过氧化值和酸价也没有明显变化;蛋糕的各主要配料成分也不会对微藻DHA的稳定性有明显影响.试验的微藻DHA可用于蛋糕的生产,从而开发新型的富含DHA的功能性食品.

  3. Maternal and fetal brain contents of docosahexaenoic acid (DHA) and arachidonic acid (AA) at various essential fatty acid (EFA), DHA and AA dietary intakes during pregnancy in mice

    NARCIS (Netherlands)

    van Goor, Saskia A; Dijck-Brouwer, D A Janneke; Fokkema, M Rebecca; van der Iest, Theo Hans; Muskiet, Frits A J

    2008-01-01

    We investigated essential fatty acids (EFA) and long-chain polyunsaturated fatty acids (LCP) in maternal and fetal brain as a function of EFA/LCP availability to the feto-maternal unit in mice. Diets varying in parent EFA, arachidonic acid (AA), and docosahexaenoic acid (DHA) were administered from

  4. Dopamine receptor alterations in female rats with diet-induced decreased brain docosahexaenoic acid (DHA): interactions with reproductive status

    OpenAIRE

    Davis, Paul F.; Ozias, Marlies K.; Carlson, Susan E.; Reed, Gregory A.; Winter, Michelle K; McCarson, Kenneth E.; Levant, Beth

    2010-01-01

    Decreased tissue levels of n-3 (omega-3) fatty acids, particularly docosahexaenoic acid (DHA), are implicated in the etiologies of non-puerperal and postpartum depression. This study examined the effects of a diet-induced loss of brain DHA content and concurrent reproductive status on dopaminergic parameters in adult female Long–Evans rats. An α-linolenic acid-deficient diet and breeding protocols were used to produce virgin and parous female rats with cortical phospholipid DHA levels 20–22% ...

  5. Thraustochytrids as production organisms for docosahexaenoic acid (DHA), squalene, and carotenoids.

    Science.gov (United States)

    Aasen, Inga Marie; Ertesvåg, Helga; Heggeset, Tonje Marita Bjerkan; Liu, Bin; Brautaset, Trygve; Vadstein, Olav; Ellingsen, Trond E

    2016-05-01

    Thraustochytrids have been applied for industrial production of the omega-3 fatty acid docosahexaenoic (DHA) since the 1990s. During more than 20 years of research on this group of marine, heterotrophic microorganisms, considerable increases in DHA productivities have been obtained by process and medium optimization. Strains of thraustochytrids also produce high levels of squalene and carotenoids, two other commercially interesting compounds with a rapidly growing market potential, but where yet few studies on process optimization have been reported. Thraustochytrids use two pathways for fatty acid synthesis. The saturated fatty acids are produced by the standard fatty acid synthesis, while DHA is synthesized by a polyketide synthase. However, fundamental knowledge about the relationship between the two pathways is still lacking. In the present review, we extract main findings from the high number of reports on process optimization for DHA production and interpret these in the light of the current knowledge of DHA synthesis in thraustochytrids and lipid accumulation in oleaginous microorganisms in general. We also summarize published reports on squalene and carotenoid production and review the current status on strain improvement, which has been hampered by the yet very few published genome sequences and the lack of tools for gene transfer to the organisms. As more sequences now are becoming available, targets for strain improvement can be identified and open for a system-level metabolic engineering for improved productivities. PMID:27041691

  6. Deptermination of Partial Molar Volumes of EPA and DHA Ethyl Esters in Supercritical Carbon Dioxide

    Institute of Scientific and Technical Information of China (English)

    MeiHUANG; XianDaWANG; 等

    2002-01-01

    The use of supercritical-fluid shromatogrphy for determining partial molar volumes of ethyl esters of cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and cis-4,7,10,13,16,19-docosa-hexaenoic acid(DHA) in supercritical carbon dioxide is presented and discussed. Partial molar volumes of EPA and DHA esters are obtained from the variation of the retention properties with the density of mobile phase at 313.15K,323.15K,333.15K and in the pressure range from 9 MPa to 21 MPa.

  7. Determination of Partial Molar Volumes of EPA and DHA Ethyl Esters in Supercritical Carbon Dioxide

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The use of supercritical-fluid chromatography for determining partial molar volumes of ethyl esters of cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and cis -4,7,10,13,16,19- docosa-hexaenoic acid (DHA) in supercritical carbon dioxide is presented and discussed. Partial molar volumes of EPA and DHA esters are obtained from the variation of the retention properties with the density of mobile phase at 313.15 K, 323.15 K, 333.15 K and in the pressure range from 9 MPa to 21 MPa.

  8. Chemoprevention of DMBA-induced mammary carcinogenesis in rats by low-dose EPA and DHA.

    OpenAIRE

    Noguchi, M.; Minami, M; Yagasaki, R.; Kinoshita, K; Earashi, M.; Kitagawa, H; Taniya, T.; Miyazaki, I.

    1997-01-01

    We investigated the effects of low-dose eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the incidence and growth of 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinoma in rats fed a high-fat (HF) diet. We also examined the effects of these treatments on the fatty acid composition of tumour and serum. Tumour incidence was significantly decreased by the administration of low-dose EPA and DHA, whereas their inhibitory effects on tumour growth did not reach significance....

  9. DHA Production in Escherichia coli by Expressing Reconstituted Key Genes of Polyketide Synthase Pathway from Marine Bacteria.

    Science.gov (United States)

    Peng, Yun-Feng; Chen, Wen-Chao; Xiao, Kang; Xu, Lin; Wang, Lian; Wan, Xia

    2016-01-01

    The gene encoding phosphopantetheinyl transferase (PPTase), pfaE, a component of the polyketide synthase (PKS) pathway, is crucial for the production of docosahexaenoic acid (DHA, 22:6ω3), along with the other pfa cluster members pfaA, pfaB, pfaC and pfaD. DHA was produced in Escherichia coli by co-expressing pfaABCD from DHA-producing Colwellia psychrerythraea 34H with one of four pfaE genes from bacteria producing arachidonic acid (ARA, 20:4ω6), eicosapentaenoic acid (EPA, 20:5ω3) or DHA, respectively. Substitution of the pfaE gene from different strain source in E. coli did not influence the function of the PKS pathway producing DHA, although they led to different DHA yields and fatty acid profiles. This result suggested that the pfaE gene could be switchable between these strains for the production of DHA. The DHA production by expressing the reconstituted PKS pathway was also investigated in different E. coli strains, at different temperatures, or with the treatment of cerulenin. The highest DHA production, 2.2 mg of DHA per gram of dry cell weight or 4.1% of total fatty acids, was obtained by co-expressing pfaE(EPA) from the EPA-producing strain Shewanella baltica with pfaABCD in DH5α. Incubation at low temperature (10-15°C) resulted in higher accumulation of DHA compared to higher temperatures. The addition of cerulenin to the medium increased the proportion of DHA and saturated fatty acids, including C12:0, C14:0 and C16:0, at the expense of monounsaturated fatty acids, including C16:1 and C18:1. Supplementation with 1 mg/L cerulenin resulted in the highest DHA yield of 2.4 mg/L upon co-expression of pfaE(DHA) from C. psychrerythraea. PMID:27649078

  10. FADS genotype and diet are important determinants of DHA status: a cross-sectional study in Danish infants1-3

    DEFF Research Database (Denmark)

    Harsløf, Laurine Bente Schram; Larsen, Lesli Hingstrup; Ritz, Christian;

    2013-01-01

    Background: Infant docosahexaenoic acid (DHA) status is supported by the DHA content of breast milk and thus can decrease once complementary feeding begins. Furthermore, it is unclear to what extent endogenous DHA synthesis contributes to status. Objective: We investigated several determinants......, breastfeeding, and fish intake explained 25% of the variation in infant RBC DHA status [mean ± SD: 6.6 ± 1.9% of fatty acids (FA%)]. Breastfeeding explained most of the variation (∼20%), and still being breastfed at 9 mo was associated with a 0.7 FA% higher DHA compared with no longer being breastfed (P ....0 FA% (P = 0.001), respectively. Each 10-g increment in fish intake was associated with an increased DHA status of 0.3 FA%. At 3 y, fish intake was the only significant determinant of DHA status (0.2 FA%/10 g). Conclusion: Breastfeeding, FADS genotype, and fish intake are important determinants of DHA...

  11. Engineering of EPA/DHA omega-3 fatty acid production by Lactococcus lactis subsp. cremoris MG1363.

    Science.gov (United States)

    Amiri-Jami, Mitra; Lapointe, Gisele; Griffiths, Mansel W

    2014-04-01

    Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been shown to be of major importance in human health. Therefore, these essential polyunsaturated fatty acids have received considerable attention in both human and farm animal nutrition. Currently, fish and fish oils are the main dietary sources of EPA/DHA. To generate sustainable novel sources for EPA and DHA, the 35-kb EPA/DHA synthesis gene cluster was isolated from a marine bacterium, Shewanella baltica MAC1. To streamline the introduction of the genes into food-grade microorganisms such as lactic acid bacteria, unnecessary genes located upstream and downstream of the EPA/DHA gene cluster were deleted. Recombinant Escherichia coli harboring the 20-kb gene cluster produced 3.5- to 6.1-fold more EPA than those carrying the 35-kb DNA fragment coding for EPA/DHA synthesis. The 20-kb EPA/DHA gene cluster was cloned into a modified broad-host-range low copy number vector, pIL252m (4.7 kb, Ery) and expressed in Lactococcus lactis subsp. cremoris MG1363. Recombinant L. lactis produced DHA (1.35 ± 0.5 mg g(-1) cell dry weight) and EPA (0.12 ± 0.04 mg g(-1) cell dry weight). This is believed to be the first successful cloning and expression of EPA/DHA synthesis gene cluster in lactic acid bacteria. Our findings advance the future use of EPA/DHA-producing lactic acid bacteria in such applications as dairy starters, silage adjuncts, and animal feed supplements. PMID:24389665

  12. Determinants of DHA status and functional effects on metabolic markers and immune modulation in early life

    DEFF Research Database (Denmark)

    Harsløf, Laurine Bente Schram

    intake and other potential determinants in infancy and childhood. The first part of the PhD thesis describes several potential determinants of infant and young child DHA status including genetic variation in FADS, breastfeeding and fish intake. Results can be found in Paper 1. Evidence for effects of n-3...

  13. Impact of Genotype on EPA and DHA Status and Responsiveness to Increased Intakes.

    Science.gov (United States)

    Minihane, Anne Marie

    2016-03-01

    At a population level, cardioprotective and cognitive actions of the fish oil (FO) derived long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been extensively demonstrated. In addition to dietary intake, which is limited for many individuals, EPA and DHA status is dependent on the efficiency of their biosynthesis from α-linolenic acid. Gender and common gene variants have been identified as influencing the rate-limiting desaturase and elongase enzymes. Response to a particular intake or status is also highly heterogeneous and likely influenced by genetic variants which impact on EPA and DHA metabolism and tissue partitioning, transcription factor activity, or physiological end-point regulation. Here, available literature relating genotype to tissue LC n-3 PUFA status and response to FO intervention is considered. It is concluded that the available evidence is relatively limited, with much of the variability unexplained, though APOE and FADS genotypes are emerging as being important. Although genotype × LC n-3 PUFA interactions have been described for a number of phenotypes, few have been confirmed in independent studies. A more comprehensive understanding of the genetic, physiological and behavioural modulators of EPA and DHA status and response to intervention is needed to allow refinement of current dietary LC n-3 PUFA recommendations and stratification of advice to "vulnerable" and responsive subgroups. PMID:26950146

  14. DHA and EPA Content and Fatty Acid Profile of 39 Food Fishes from India.

    Science.gov (United States)

    Mohanty, Bimal Prasanna; Ganguly, Satabdi; Mahanty, Arabinda; Sankar, T V; Anandan, R; Chakraborty, Kajal; Paul, B N; Sarma, Debajit; Syama Dayal, J; Venkateshwarlu, G; Mathew, Suseela; Asha, K K; Karunakaran, D; Mitra, Tandrima; Chanda, Soumen; Shahi, Neetu; Das, Puspita; Das, Partha; Akhtar, Md Shahbaz; Vijayagopal, P; Sridhar, N

    2016-01-01

    Docosahexaenoic acid (DHA) is the principal constituent of a variety of cells especially the brain neurons and retinal cells and plays important role in fetal brain development, development of motor skills, and visual acuity in infants, lipid metabolism, and cognitive support and along with eicosapentaenoic acid (EPA) it plays important role in preventing atherosclerosis, dementia, rheumatoid arthritis, Alzheimer's disease, and so forth. Being an essential nutrient, it is to be obtained through diet and therefore searching for affordable sources of these ω-3 polyunsaturated fatty acids (PUFA) is important for consumer guidance and dietary counseling. Fish is an important source of PUFA and has unique advantage that there are many food fish species available and consumers have a wide choice owing to availability and affordability. The Indian subcontinent harbors a rich fish biodiversity which markedly varies in their nutrient composition. Here we report the DHA and EPA content and fatty acid profile of 39 important food fishes (including finfishes, shellfishes, and edible molluscs from both marine water and freshwater) from India. The study showed that fishes Tenualosa ilisha, Sardinella longiceps, Nemipterus japonicus, and Anabas testudineus are rich sources of DHA and EPA. Promotion of these species as DHA rich species would enhance their utility in public health nutrition. PMID:27579313

  15. DHA and EPA Content and Fatty Acid Profile of 39 Food Fishes from India

    Directory of Open Access Journals (Sweden)

    Bimal Prasanna Mohanty

    2016-01-01

    Full Text Available Docosahexaenoic acid (DHA is the principal constituent of a variety of cells especially the brain neurons and retinal cells and plays important role in fetal brain development, development of motor skills, and visual acuity in infants, lipid metabolism, and cognitive support and along with eicosapentaenoic acid (EPA it plays important role in preventing atherosclerosis, dementia, rheumatoid arthritis, Alzheimer’s disease, and so forth. Being an essential nutrient, it is to be obtained through diet and therefore searching for affordable sources of these ω-3 polyunsaturated fatty acids (PUFA is important for consumer guidance and dietary counseling. Fish is an important source of PUFA and has unique advantage that there are many food fish species available and consumers have a wide choice owing to availability and affordability. The Indian subcontinent harbors a rich fish biodiversity which markedly varies in their nutrient composition. Here we report the DHA and EPA content and fatty acid profile of 39 important food fishes (including finfishes, shellfishes, and edible molluscs from both marine water and freshwater from India. The study showed that fishes Tenualosa ilisha, Sardinella longiceps, Nemipterus japonicus, and Anabas testudineus are rich sources of DHA and EPA. Promotion of these species as DHA rich species would enhance their utility in public health nutrition.

  16. DHA与EPA的研究进展%Progress in research on DHA and EPA

    Institute of Scientific and Technical Information of China (English)

    左珊珊

    2012-01-01

    二十二碳六烯酸(Docosahexaenoic acid,22:6n3,DHA)和二十碳五烯酸(Eicosapentaenoic acid,20∶5n3,EPA)均属于n3类多不饱和脂肪酸(Polyunsatumted fatty acids,PUFAs),能够在一定程度上预防和治疗糖尿病、类风湿性关节炎、自身免疫紊乱等疾病,对人体健康非常重要,因而受到越来越多的关注.本文对DHA与EPA的生理功能、合成机制及其在微藻和真菌中合成的研究现状进行了综述,并对利用转基因技术在哺乳动物体内生产EPA和DHA的前景进行了展望.%Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are both n3 polyunsatumted fatty acids, which prevent and treat some diseases including diabetes mellitus, rheumatoid arthritis (RA) and autoimmune disorder to a certain extent and are essential for human health, thus are paid more and more attention. This paper reviews the physiological functions and syn-thetic mechanisms of DHA and EPA, as well as their roles in syntheses of microalgae and fungi, and prospected the syntheses of EPA and DHA in mammals by transgenic technology.

  17. Production of lipids and docosahexasaenoic acid (DHA) by a native Thraustochytrium strain

    DEFF Research Database (Denmark)

    Shene, Carolina; Leyton, Allison; Rubilar, Mónica;

    2013-01-01

    Production of docosahexasaenoic acid (DHA) by a native Labyrinthulomycetes strain, Thraustochytriidae sp. TN5, whose growth characteristics present some differences to related strains, was scaled from shaken flask to a laboratory fermentor. The effect of the growth medium composition and growth c...

  18. Trans isomers of EPA and DHA in omega-3 products on the European market.

    Science.gov (United States)

    Sciotto, Caterina; Mjøs, Svein A

    2012-07-01

    The levels of trans isomers of eicosapentaenoic (EPA) acid and docosahexaenoic acid (DHA) in 77 omega-3 products on the European market have been studied. Fatty acids were analyzed as fatty acid methyl esters by gas chromatography with a flame ionization detector, using a cyanopropyl coated stationary phase. The amount of mono-trans EPA isomers relative to the corresponding all-cis isomer ranged from 0.19 to 4.5 %. The corresponding values for mono-trans DHA relative to the all-cis isomer ranged from 0.25 to 5.9 %. There was a strong correlation between the degree of isomerization of EPA and DHA, showing that DHA was 1.26 times more isomerized than EPA. Division of the samples into different product groups showed that samples with a low degree of isomerization were found in all groups, except one. This shows that a high degree of isomerization is avoidable, and also points to deodorization of the oils as the main source of trans isomers. PMID:22566205

  19. Impact of Genotype on EPA and DHA Status and Responsiveness to Increased Intakes

    Directory of Open Access Journals (Sweden)

    Anne Marie Minihane

    2016-03-01

    Full Text Available At a population level, cardioprotective and cognitive actions of the fish oil (FO derived long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs eicosapentaenoic acid (EPA and docosahexaenoic acid (DHA have been extensively demonstrated. In addition to dietary intake, which is limited for many individuals, EPA and DHA status is dependent on the efficiency of their biosynthesis from α-linolenic acid. Gender and common gene variants have been identified as influencing the rate-limiting desaturase and elongase enzymes. Response to a particular intake or status is also highly heterogeneous and likely influenced by genetic variants which impact on EPA and DHA metabolism and tissue partitioning, transcription factor activity, or physiological end-point regulation. Here, available literature relating genotype to tissue LC n-3 PUFA status and response to FO intervention is considered. It is concluded that the available evidence is relatively limited, with much of the variability unexplained, though APOE and FADS genotypes are emerging as being important. Although genotype × LC n-3 PUFA interactions have been described for a number of phenotypes, few have been confirmed in independent studies. A more comprehensive understanding of the genetic, physiological and behavioural modulators of EPA and DHA status and response to intervention is needed to allow refinement of current dietary LC n-3 PUFA recommendations and stratification of advice to “vulnerable” and responsive subgroups.

  20. Effect of DHA-containing formula on growth of preterm infants to 59 weeks postmenstrual age.

    Science.gov (United States)

    Ryan, Alan S.; Montalto, Michael B.; Groh-Wargo, Sharon; Mimouni, Francis; Sentipal-Walerius, Joan; Doyle, Jeanine; Siegman, Joel S.; Thomas, Alicia J.

    1999-01-01

    Between May 1993, and September 1994, a randomized, blinded clinical trial was conducted to evaluate measures of growth and body composition in 63 (32 males; 31 females) healthy, low-birth-weight infants (940-2250 g) who were randomly assigned to an infant formula with docosahexaenoic acid (22:6n3, DHA, 0.2 wt%) from fish oil or to a control formula. A preterm formula with or without DHA was fed beginning at 7-10 days prior to hospital discharge through 43 weeks postmenstrual age (PMA). Then, from 43-59 weeks PMA, infants were fed a term infant formula with or without a corresponding amount of DHA. Growth (weight, length, head circumference), regional body fatness (triceps, subscapular, suprailiac skinfold thicknesses), circumferences (arm, abdominal, chest), and estimates of body composition determined by total body electrical conductivity (TOBEC) (fat-free mass [FFM]) were evaluated. Growth was slower in males fed the DHA formula. They had significantly (P weight, length, and head circumference between study enrollment to 59 weeks PMA than those fed the control formula. At 51 weeks PMA, males in the DHA group had significantly smaller head circumferences (P head circumferences (P body weight (kcal/kg/d), mean energy intakes from formula at 51 and 59 weeks PMA were not significantly different between feeding groups. The differences in recumbent length, head circumference, and FFM remained statistically significant after controlling for energy and protein intakes (P body fat (TBF), when expressed as a percentage of total body weight, differed significantly between feeding groups. Among females, there were no significant differences between the feeding groups in measures of growth, body composition, or energy intake. The results indicated that infant formula with fish oil containing DHA and EPA in a 5:1 ratio had a significant, negative effect on growth and body composition in males during the first 6 months of life. It is not clear why the growth deficits were

  1. Respiration gating and Bloch fitting improve pH measurements with acidoCEST MRI in an ovarian orthotopic tumor model

    Science.gov (United States)

    Jones, Kyle M.; Randtke, Edward A.; Howison, Christine M.; Pagel, Mark D.

    2016-03-01

    We have developed a MRI method that can measure extracellular pH in tumor tissues, known as acidoCEST MRI. This method relies on the detection of Chemical Exchange Saturation Transfer (CEST) of iopamidol, an FDA-approved CT contrast agent that has two CEST signals. A log10 ratio of the two CEST signals is linearly correlated with pH, but independent of agent concentration, endogenous T1 relaxation time, and B1 inhomogeneity. Therefore, detecting both CEST effects of iopamidol during in vivo studies can be used to accurately measure the extracellular pH in tumor tissues. Past in vivo studies using acidoCEST MRI have suffered from respiration artifacts in orthotopic and lung tumor models that have corrupted pH measurements. In addition, the non-linear fitting method used to analyze results is unreliable as it is subject to over-fitting especially with noisy CEST spectra. To improve the technique, we have recently developed a respiration gated CEST MRI pulse sequence that has greatly reduced motion artifacts, and we have included both a prescan and post scan to remove endogenous CEST effects. In addition, we fit the results by parameterizing the contrast of the exogenous agent with respect to pH via the Bloch equations modified for chemical exchange, which is less subject to over-fitting than the non-linear method. These advances in the acidoCEST MRI technique and analysis methods have made pH measurements more reliable, especially in areas of the body subject to respiratory motion.

  2. DHA supplemented in peptamen diet offers no advantage in pathways to amyloidosis: is it time to evaluate composite lipid diet?

    Directory of Open Access Journals (Sweden)

    Zareen Amtul

    Full Text Available Numerous reports have documented the beneficial effects of dietary docosahexaenoic acid (DHA on beta-amyloid production and Alzheimer's disease (AD. However, none of these studies have examined and compared DHA, in combination with other dietary nutrients, for its effects on plaque pathogenesis. Potential interactions of DHA with other dietary nutrients and fatty acids are conventionally ignored. Here we investigated DHA with two dietary regimes; peptamen (pep+DHA and low fat diet (low fat+DHA. Peptamen base liquid diet is a standard sole-source nutrition for patients with gastrointestinal dysfunction. Here we demonstrate that a robust AD transgenic mouse model shows an increased tendency to produce beta-amyloid peptides and amyloid plaques when fed a pep+DHA diet. The increase in beta-amyloid peptides was due to an elevated trend in the levels of beta-secretase amyloid precursor protein (APP cleaving enzyme (BACE, the proteolytic C-terminal fragment beta of APP and reduced levels of insulin degrading enzyme that endoproteolyse beta-amyloid. On the contrary, TgCRND8 mice on low fat+DHA diet (based on an approximately 18% reduction of fat intake ameliorate the production of abeta peptides and consequently amyloid plaques. Our work not only demonstrates that DHA when taken with peptamen may have a tendency to confer a detrimental affect on the amyloid plaque build up but also reinforces the importance of studying composite lipids or nutrients rather than single lipids or nutrients for their effects on pathways important to plaque development.

  3. The effects of aspirin on platelet function and lysophosphatidic acids depend on plasma concentrations of EPA and DHA

    OpenAIRE

    Block, Robert C.; Abdolahi, Amir; Tu, Xin; Georas, Steve N.; Brenna, J. Thomas; Phipps, Richard P.; Lawrence, Peter; Mousa, Shaker A.

    2014-01-01

    Aspirin’s prevention of cardiovascular disease (CVD) events in individuals with type 2 diabetes mellitus is controversial. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and aspirin all affect the cyclooxygenase enzyme. The relationship between plasma EPA and DHA and aspirin’s effects has not been determined. Thirty adults with type 2 diabetes mellitus ingested aspirin (81 mg/day) for 7 days, then EPA+DHA (2.6 g/day) for 28 days, then both for another 7 days. Lysophosphatidic acid...

  4. The Effects of EPA, DHA, and Aspirin Ingestion on Plasma Lysophospholipids and Autotaxin

    OpenAIRE

    Block, RC; Duff, R; Lawrence, P.; Kakinami, L.; Brenna, JT; Shearer, GC; Meednu, N; Mousa, S; Friedman, A.; Harris, WS; Larson, Mark; Georas, S

    2010-01-01

    Lysophophatidylcholine (LPC) and lysophosphatidic acid (LPA) are potent lysolipid mediators increasingly linked with atherosclerosis and inflammation. A current model proposing that plasma LPA is produced when LPC is hydrolyzed by the enzyme autotaxin has not been rigorously investigated in human subjects. We conducted a clinical trial of eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) and aspirin ingestion in normal volunteers. Fasting blood samples were drawn at baseline and after 4-we...

  5. Is the omega-3 index a valid marker of intestinal membrane phospholipid EPA+DHA content?

    OpenAIRE

    Gurzell, Eric A.; Wiesinger, Jason; Morkam, Christina; Hemmrich, Sophia; William S. Harris; Fenton, Jenifer I.

    2014-01-01

    Despite numerous studies investigating n-3 long chain polyunsaturated fatty acid (LCPUFA) supplementation and inflammatory bowel diseases (IBD), the extent to which dietary n-3 LCPUFAs incorporate in gastrointestinal (GI) tissues and correlate to the omega-3 index is unknown. In this study, mice were fed three diets with increasing percent of energy (%en) derived from eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA). Dietary levels reflected recommended intakes of fish/fish oil by the...

  6. Peningkatan EPA dan DHA Rotifer (Brachionus plicatilis) oleh Bacillus sp. dengan Periode Pengkayaan Berbeda

    OpenAIRE

    Budi, Sutia; Zainuddin; Aslamyah, Siti

    2012-01-01

    Brachionus plicatilis memiliki beberapa kelebihan dibanding dengan pakan alami lainnya, seperti ukurannya yang relatif kecil, tetap bertahan di kolom air dan tidak mengendap, bergerak dengan kecepatan yang rendah dan laju perkembangbiakan yang cukup tinggi, merupakan pakan alami yang banyak dipergunakan usaha pembenihan. Penelitian ini bertujuan untuk melihat pengaruh pengkayaan dengan menambahkan Bacillus sp. Pada media kultur terhadap EPA dan DHA Nannochloropsis sp. dengan kepadatan 105 ce...

  7. TRANSLATIONAL STUDIES ON REGULATION OF BRAIN DOCOSAHEXAENOIC ACID (DHA) METABOLISM IN VIVO

    OpenAIRE

    Rapoport, Stanley I.

    2012-01-01

    One goal in the field of brain polyunsaturated fatty acid (PUFA) metabolism is to translate the many studies that have been conducted in vitro and in animal models to the clinical setting. Doing so should elucidate the role of PUFAs in the human brain, and effects of diet, drugs, disease and genetics. This review briefly discusses new in vivo radiotracer kinetic and neuroimaging techniques that allow us to do this, with a focus on docosahexaenoic acid (DHA). We illustrate how brain PUFA metab...

  8. Nonlinear Mathematical Simulation and Analysis of Dha Regulon for Glycerol Metabolism in Klebsiella pneumoniae

    Institute of Scientific and Technical Information of China (English)

    孙亚琴; 叶剑雄; 牟晓佳; 滕虎; 冯恩民; 曾安平; 修志龙

    2012-01-01

    Glycerol may be converted to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae under anaerobic conditions and glycerol dismutation involves two parallel pathways controlled by the dha regulon. In this study, a fourteen-dimensional nonlinear dynamic system is presented to describe the continuous culture and multiplicity analysis, in which two regulated negative-feedback mechanisms of repression and enzyme inhibition are investigated. The model describing the expression of gene-mRNA-enzyme-product was established according to the repression of the dha regulon by 3-hydroxypropionaldehy (3-HPA). Comparisons between simulated and experimental results indicate that the model can be used to describe the production of 1,3-PD under continuous fermentation. The new model is translated into the corresponding S-system version. The robustness of this model is discussed by using the S-system model and the sensitivity analysis shows that the model is sufficiently robust. The influences of initial glycerol concentration and dilution rate on the biosynthesis of 1,3-PD and the stability of the dha regulon model are investigated. The intracellular concentrations of glycerol, 1,3-PD, 3-HPA, repressor mRNA, repressor, mRNA and protein levels of glycerol dehydratase (GDHt) and 1,3-PD oxydoreductase (PDOR) can be predicted for continuous cultivation. The results of simulation and analysis indicate that 3-HPA accumulation will repress the expression of the dha regulon at the transcriptional level. This model gives new insights into the regulation of glycerol metabolism in K. pneumoniae and explain some of the experimental observations.

  9. Effect of different mulch materials on the soil dehydrogenase activity (DHA) in an organic pepper crop

    Science.gov (United States)

    Moreno, Marta M.; Peco, Jesús; Campos, Juan; Villena, Jaime; González, Sara; Moreno, Carmen

    2016-04-01

    The use biodegradable materials (biopolymers of different composition and papers) as an alternative to conventional mulches has increased considerably during the last years mainly for environmental reason. In order to assess the effect of these materials on the soil microbial activity during the season of a pepper crop organically grown in Central Spain, the soil dehydrogenase activity (DHA) was measured in laboratory. The mulch materials tested were: 1) black polyethylene (PE, 15 μm); black biopolymers (15 μm): 2) Mater-Bi® (corn starch based), 3) Sphere 4® (potato starch based), 4) Sphere 6® (potato starch based), 5) Bioflex® (polylactic acid based), 6) Ecovio® (polylactic acid based), 7) Mimgreen® (black paper, 85 g/m2). A randomized complete block design with four replications was adopted. The crop was drip irrigated following the water demand of each treatment. Soil samples (5-10 cm depth) under the different mulches were taken at different dates (at the beginning of the crop cycle and at different dates throughout the crop season). Additionally, samples of bare soil in a manual weeding and in an untreated control were taken. The results obtained show the negative effect of black PE on the DHA activity, mainly as result of the higher temperature reached under the mulch and the reduction in the gas interchange between the soil and the atmosphere. The values corresponding to the biodegradable materials were variable, although highlighting the low DHA activity observed under Bioflex®. In general, the uncovered treatments showed higher values than those reached under mulches, especially in the untreated control. Keywords: mulch, biodegradable, biopolymer, paper, dehydrogenase activity (DHA). Acknowledgements: the research was funded by Project RTA2011-00104-C04-03 from the INIA (Spanish Ministry of Economy and Competitiveness).

  10. A computational study of the phosphoryl transfer reaction between ATP and Dha in aqueous solution.

    Science.gov (United States)

    Bordes, I; Ruiz-Pernía, J J; Castillo, R; Moliner, V

    2015-10-28

    Phosphoryl transfer reactions are ubiquitous in biology, being involved in processes ranging from energy and signal transduction to the replication genetic material. Dihydroxyacetone phosphate (Dha-P), an intermediate of the synthesis of pyruvate and a very important building block in nature, can be generated by converting free dihydroxyacetone (Dha) through the action of the dihydroxyacetone kinase enzyme. In this paper the reference uncatalyzed reaction in solution has been studied in order to define the foundations of the chemical reaction and to determine the most adequate computational method to describe this electronically complex reaction. In particular, the phosphorylation reaction mechanism between adenosine triphosphate (ATP) and Dha in aqueous solution has been studied by means of quantum mechanics/molecular mechanics (QM/MM) Molecular Dynamics (MD) simulations with the QM subset of atoms described with semi-empirical and DFT methods. The results appear to be strongly dependent on the level of calculation, which will have to be taken into account for future studies of the reaction catalyzed by enzymes. In particular, PM3/MM renders lower free energy barriers and a less endergonic process than AM1d/MM and PM6/MM methods. Nevertheless, the concerted pathway was not located with the former combination of potentials. PMID:26303076

  11. Scientific Opinion on the Tolerable Upper Intake Level of eicosapentaenoic acid (EPA, docosahexaenoic acid (DHA and docosapentaenoic acid (DPA

    Directory of Open Access Journals (Sweden)

    EFSA Panel on Dietetic Products, Nutrition and Allergies

    2012-07-01

    Full Text Available

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to deliver a scientific opinion on the Tolerable Upper Intake Level (UL of the n-3 LCPUFAs eicosapentaenoic acid (EPA, docosahexaenoic acid (DHA and docosapentaenoic acid (DPA. Available data are insufficient to establish a UL for n-3 LCPUFA (individually or combined for any population group. At observed intake levels, consumption of n-3 LCPUFA has not been associated with adverse effects in healthy children or adults. Long-term supplemental intakes of EPA and DHA combined up to about 5 g/day do not appear to increase the risk of spontaneous bleeding episodes or bleeding complications, or affect glucose homeostasis immune function or lipid peroxidation, provided the oxidative stability of the n-3 LCPUFAs is guaranteed. Supplemental intakes of EPA and DHA combined at doses of 2 6 g/day, and of DHA at doses of 2 4 g/day, induce an increase in LDL-cholesterol concentrations of about 3 % which may not have an adverse effect on cardiovascular disease risk, whereas EPA at doses up to 4 g/day has no significant effect on LDL cholesterol. Supplemental intakes of EPA and DHA combined at doses up to 5 g/day, and supplemental intakes of EPA alone up to 1.8 g/day, do not raise safety concerns for adults. Dietary recommendations for EPA and DHA based on cardiovascular risk considerations for European adults are between 250 and 500 mg/day. Supplemental intakes of DHA alone up to about 1 g/day do not raise safety concerns for the general population. No data are available for DPA when consumed alone. In the majority of the human studies considered, fish oils, also containing DPA in generally unknown (but relatively low amounts, were the source of EPA and DHA.

  12. DHA is a more potent inhibitor of breast cancer metastasis to bone and related osteolysis than EPA

    OpenAIRE

    Rahman, M.; Veigas, Maria; Williams, Paul J.; Fernandes, Gabriel

    2013-01-01

    Breast cancer patients often develop bone metastasis evidenced by osteolytic lesions, leading to severe pain and bone fracture. Attenuation of breast cancer metastasis to bone and associated osteolysis by fish oil (FO), rich in EPA and DHA, has been demonstrated previously. However, it was not known whether EPA and DHA differentially or similarly affect breast cancer bone metastasis and associated osteolysis. In vitro culture of parental and luciferase gene encoded MDA-MB-231 human breast can...

  13. Concentration of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) of Asian catfish oil by urea complexation: optimization of reaction conditions

    OpenAIRE

    Pornpisanu Thammapat; Sirithon Siriamornpun; Patcharin Raviyan

    2016-01-01

    Optimization of the concentrating conditions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) extracted from Asian catfish oil was studied to obtain a maximum concentration. The crude fish oil was extracted from the belly flap and adipose tissue of Asian catfish, and the extracted oil was used as fresh crude oil. The EPA and DHA were concentrated by the urea complexation method. A hexagonal rotatable design was applied to examine the effects of crystallization temperatur...

  14. DHA and EPA reverse cystic fibrosis-related FA abnormalities by suppressing FA desaturase expression and activity

    OpenAIRE

    Njoroge, Sarah W; Laposata, Michael; Katrangi, Waddah; Seegmiller, Adam C.

    2012-01-01

    Patients and models of cystic fibrosis (CF) exhibit consistent abnormalities of polyunsaturated fatty acid composition, including decreased linoleate (LA) and docosahexaenoate (DHA) and variably increased arachidonate (AA), related in part to increased expression and activity of fatty acid desaturases. These abnormalities and the consequent CF-related pathologic manifestations can be reversed in CF mouse models by dietary supplementation with DHA. However, the mechanism is unknown. This study...

  15. DHA protects against experimental colitis in IL-10-deficient mice associated with the modulation of intestinal epithelial barrier function.

    Science.gov (United States)

    Zhao, Jie; Shi, Peiliang; Sun, Ye; Sun, Jing; Dong, Jian-Ning; Wang, Hong-Gang; Zuo, Lu-Gen; Gong, Jian-Feng; Li, Yi; Gu, Li-Li; Li, Ning; Li, Jie-Shou; Zhu, Wei-Ming

    2015-07-01

    A defect in the intestinal barrier is one of the characteristics of Crohn's disease (CD). The tight junction (TJ) changes and death of epithelial cells caused by intestinal inflammation play an important role in the development of CD. DHA, a long-chain PUFA, has been shown to be helpful in treating inflammatory bowel disease in experimental models by inhibiting the NF-κB pathway. The present study aimed at investigating the specific effect of DHA on the intestinal barrier function in IL-10-deficient mice. IL-10-deficient mice (IL-10(-/-)) at 16 weeks of age with established colitis were treated with DHA (i.g. 35.5 mg/kg per d) for 2 weeks. The severity of their colitis, levels of pro-inflammatory cytokines, epithelial gene expression, the distributions of TJ proteins (occludin and zona occludens (ZO)-1), and epithelial apoptosis in the proximal colon were measured at the end of the experiment. DHA treatment attenuated the established colitis and was associated with reduced infiltration of inflammatory cells in the colonic mucosa, lower mean histological scores and decreased levels of pro-inflammatory cytokines (IL-17, TNF-α and interferon-γ). Moreover, enhanced barrier function was observed in the DHA-treated mice that resulted from attenuated colonic permeability, rescued expression and corrected distributions of occludin and ZO-1. The results of the present study indicate that DHA therapy may ameliorate experimental colitis in IL-10(-/-) mice by improving the intestinal epithelial barrier function.

  16. Treatment with DHA/EPA ameliorates atopic dermatitis-like skin disease by blocking LTB4 production.

    Science.gov (United States)

    Yoshida, Shinya; Yasutomo, Koji; Watanabe, Toshiyuki

    2016-01-01

    Atopic dermatitis (AD) is caused by both dysregulated immune responses and an impaired skin barrier. Although leukotriene B4 (LTB4) is involved in tissue inflammation that occurs in several disorders, including AD, therapeutic strategies based on LTB4 inhibition have not been explored. Here we demonstrate that progression of an AD-like skin disease in NC/Nga mice is inhibited when docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) is administered together with FK506. Treatment with DHA/EPA and FK506 decreases the clinical score of dermatitis in NC/Nga mice and lowers local LTB4 concentrations. The treatment also suppressed the infiltration of T cells, B cells, eosinophils and neutrophils, and promoted reduced serum IgE levels. Secretion of IL-13 and IL-17A in CD4(+) T cells was lower in DHA/EPA- and FK506-treated mice than in mice treated with FK506 alone. The inhibition of disease progression induced by DHA/EPA was reversed by local injection of LTB4, suggesting that the therapeutic effect of DHA/EPA is LTB4-dependent. Our results demonstrate that treatment of AD with DHA/EPA is effective for allergic skin inflammation and acts by suppressing LTB4 production. J. Med. Invest. 63: 187-191, August, 2016. PMID:27644556

  17. DHA improves cognition and prevents dysfunction of entorhinal cortex neurons in 3xTg-AD mice.

    Directory of Open Access Journals (Sweden)

    Dany Arsenault

    Full Text Available Defects in neuronal activity of the entorhinal cortex (EC are suspected to underlie the symptoms of Alzheimer's disease (AD. Whereas neuroprotective effects of docosahexaenoic acid (DHA have been described, the effects of DHA on the physiology of EC neurons remain unexplored in animal models of AD. Here, we show that DHA consumption improved object recognition (↑12%, preventing deficits observed in old 3xTg-AD mice (↓12%. Moreover, 3xTg-AD mice displayed seizure-like akinetic episodes, not detected in NonTg littermates and partly prevented by DHA (↓50%. Patch-clamp recording revealed that 3xTg-AD EC neurons displayed (i loss of cell capacitance (CC, suggesting reduced membrane surface area; (ii increase of firing rate versus injected current (F-I curve associated with modified action potentials, and (iii overactivation of glutamatergic synapses, without changes in synaptophysin levels. DHA consumption increased CC (↑12% and decreased F-I slopes (↓21%, thereby preventing the opposite alterations observed in 3xTg-AD mice. Our results indicate that cognitive performance and basic physiology of EC neurons depend on DHA intake in a mouse model of AD.

  18. Differential effects of EPA versus DHA on postprandial vascular function and the plasma oxylipin profile in men[S

    Science.gov (United States)

    McManus, Seán; Tejera, Noemi; Awwad, Khader; Rigby, Neil; Fleming, Ingrid; Cassidy, Aedin; Minihane, Anne Marie

    2016-01-01

    Our objective was to investigate the impact of EPA versus DHA on arterial stiffness and reactivity and underlying mechanisms (with a focus on plasma oxylipins) in the postprandial state. In a three-arm crossover acute test meal trial, men (n = 26, 35–55 years) at increased CVD risk received a high-fat (42.4 g) test meal providing 4.16 g of EPA or DHA or control oil in random order. At 0 h and 4 h, blood samples were collected to quantify plasma fatty acids, long chain n-3 PUFA-derived oxylipins, nitrite and hydrogen sulfide, and serum lipids and glucose. Vascular function was assessed using blood pressure, reactive hyperemia index, pulse wave velocity, and augmentation index (AIx). The DHA-rich oil significantly reduced AIx by 13% (P = 0.047) with the decrease following EPA-rich oil intervention not reaching statistical significance. Both interventions increased EPA- and DHA-derived oxylipins in the acute postprandial state, with an (1.3-fold) increase in 19,20-dihydroxydocosapentaenoic acid evident after DHA intervention (P < 0.001). In conclusion, a single dose of DHA significantly improved postprandial arterial stiffness as assessed by AIx, which if sustained would be associated with a significant decrease in CVD risk. The observed increases in oxylipins provide a mechanistic insight into the AIx effect. PMID:27170732

  19. Maternal DHA supplementation protects rat offspring against impairment of learning and memory following prenatal exposure to valproic acid.

    Science.gov (United States)

    Gao, Jingquan; Wu, Hongmei; Cao, Yonggang; Liang, Shuang; Sun, Caihong; Wang, Peng; Wang, Ji; Sun, Hongli; Wu, Lijie

    2016-09-01

    Docosahexaenoic acid (22:6n-3; DHA) is known to play a critical role in postnatal brain development. However, there have been no studies investigating the preventive effect of DHA on prenatal valproic acid (VPA)-induced behavioral and molecular alterations in offspring. The present study was to evaluate the neuroprotective effects in offspring using maternal feeding of DHA to rats exposed to VPA in pregnancy. In the present study, rats were exposed to VPA on day 12.5 of pregnancy; DHA was administered at the dosages of 100, 300 and 500 mg/kg/day for 3 weeks from day 1 to 21 of pregnancy. The results showed that maternal feeding of DHA to the prenatal exposed to VPA (1) prevented VPA-induced learning and memory impairment but did not change social-related behavior, (2) increased total DHA content in offspring plasma and hippocampus, (3) rescued VPA-induced neuronal loss and apoptosis of pyramidal cells in hippocampal CA1, (4) influenced the content of malondialdehyde and glutathione and the activities of superoxide dismutase and glutathione in the hippocampus, (5) altered levels of apoptosis-related proteins (Bcl-2, Bax and caspase-3) and inhibited the activity of caspase-3 in offspring hippocampus and (6) enhanced relative levels of p-CaMKII and p-CREB proteins in the hippocampus. These findings suggest that maternal feeding with DHA may prevent prenatal VPA-induced impairment of learning and memory, normalize several different molecules associated with oxidative stress and apoptosis in the hippocampus of offspring, and exert preventive effects on prenatal VPA-induced brain dysfunction. PMID:27469996

  20. Synthesis of DHA/EPA-rich phosphatidylcholine by immobilized phospholipase A1: effect of water addition and vacuum condition.

    Science.gov (United States)

    Li, Daoming; Qin, Xiaoli; Wang, Weifei; Li, Zhigang; Yang, Bo; Wang, Yonghua

    2016-08-01

    DHA/EPA-rich phosphatidylcholine (PC) was successfully synthesized by immobilized phospholipase A1 (PLA1)-catalyzed transesterification of PC and DHA/EPA-rich ethyl esters in a solvent-free system. Effects of reaction temperature, water addition and substrate mass ratio on the incorporation of DHA/EPA were evaluated using response surface methods (RSM). Water addition had most significant effect on the incorporation. Reaction temperature and substrate mass ratio, however, had no significant effect on the incorporation. The maximal incorporation was 19.09 % (24 h) under the following conditions: temperature 55.7 °C, water addition 1.1 wt % and substrate mass ratio (ethyl esters/PC) 6.8:1. Furthermore, effects of water addition (from 0 to 1.25 wt %) on DHA/EPA incorporation and the composition of products were further investigated. The immobilized PLA1 was more active when water addition was above 0.5 wt %. By monitoring the reaction processes with different water addition, a possible reaction scheme was proposed for transesterification of PC with DHA/EPA-rich ethyl esters. In summary, PC and sn2-lysophosphatidylocholine (LPC) were predominant in the mixtures at early stages of reaction, whereas sn1-LPC and glycerophosphocholine (GPC) predominant at later stages. The vacuum employed after 24 h significantly increased the incorporation of DHA/EPA and the composition of PC, and the highest incorporation (30.31 %) of DHA/EPA was obtained at 72 h and the yield of PC was 47.2 %. PMID:27108109

  1. Síntesis de óxidos tipo perovskita, mediante polimerización con acido cítrico y combustión con glicina.

    OpenAIRE

    Gómez Cuaspud, Jairo A; Valencia Ríos, Jesús S

    2011-01-01

    En este trabajo se describe la preparación de la perovskita La0.25 Sr 0.25 CO 0.5 Fe 0.5 O3 (LSCoF), mediante la polimerización con acido cítrico y combustión con glicina, con el propósito de obtener materiales para potenciales aplicaciones catalíticas, eléctricas y electroquímicas; para ellos los sólidos se caracterizaron mediante difracción de rayos X (DRX) y microscopia electrónica de barrido (SEM); obteniendo información acerca de la formación y pureza de fases, la morfología, la estructu...

  2. Identification of DHA-23, a Novel Plasmid-mediated and Inducible AmpC beta-Lactamase from Enterobacteriaceae in Northern Taiwan

    Directory of Open Access Journals (Sweden)

    Wen-Shyang eHsieh

    2015-05-01

    Full Text Available Objectives: AmpC β-lactamases are classified as Amber Class C and Bush Group 1. AmpC β-lactamases can hydrolyze broad and extended-spectrum cephalosporins, and are not inhibited by β-lactamase inhibitors such as clavulanic acid. This study was conducted to identify DHA-23, a novel plasmid-mediated and inducible AmpC β-lactamase obtained from Enterobacteriaceae. Methods: A total of 210 carbapenem-resistant Enterobacteriaceae isolates were collected from a medical center (comprising 2 branches in Northern Taiwan during 2009–2012. AmpC β-lactamase genes were analyzed through a polymerase chain reaction using plasmid DNA templates and gene sequencing. The genetic relationships of the isolates were typed using pulsed-field gel electrophoresis following the digestion of intact genomic DNA by using XbaI. Results: Three enterobacterial isolates (one Escherichia coli and 2 Klebsiella pneumoniae were obtained from 3 hospitalized patients. All 3 isolates were resistant or intermediately susceptible to all β-lactams, and exhibited reduced susceptibility to carbapenems. These 3 isolates expressed a novel AmpC β-lactamase, designated DHA-23, approved by the curators of the Lahey website. DHA-23 differs from DHA-1 and DHA-6 by one amino acid substitution (Ser245Ala, exhibiting 2 amino acid changes compared with DHA-7 and DHA-Morganella morganii; 3 amino acid changes compared with DHA-3; 4 amino acid changes compared with DHA-5; and 8 amino acid changes compared with DHA-2 (> 97% identity. This AmpC β-lactamase is inducible using a system involving ampR. Conclusion: This is the first report to address DHA-23, a novel AmpC β-lactamase. DHA-type β-lactamases are continuous threat in Taiwan.

  3. Redox-Sensitive Induction of Src/PI3-kinase/Akt and MAPKs Pathways Activate eNOS in Response to EPA:DHA 6:1

    OpenAIRE

    Faraj Zgheel; Mahmoud Alhosin; Sherzad Rashid; Mélanie Burban; Cyril Auger; Schini-Kerth, Valérie B.

    2014-01-01

    AIMS: Omega-3 fatty acid products containing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have vasoprotective effects, in part, by stimulating the endothelial formation of nitric oxide (NO). This study determined the role of the EPA:DHA ratio and amount, and characterized the mechanism leading to endothelial NO synthase (eNOS) activation. METHODS AND RESULTS: EPA:DHA 6∶1 and 9∶1 caused significantly greater endothelium-dependent relaxations in porcine coronary artery rings than ...

  4. Exogenous modification of platelet membranes with the omega-3 fatty acids EPA and DHA reduces platelet procoagulant activity and thrombus formation

    OpenAIRE

    Larson, Mark K.; Tormoen, Garth W.; Weaver, Lucinda J.; Luepke, Kristen J.; Patel, Ishan A.; Hjelmen, Carl E.; Ensz, Nicole M.; McComas, Leah S.; McCarty, Owen J. T.

    2012-01-01

    Several studies have implicated the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in inhibition of normal platelet function, suggesting a role for platelets in EPA- and DHA-mediated cardioprotection. However, it is unclear whether the cardioprotective mechanisms arise from alterations to platelet-platelet, platelet-matrix, or platelet-coagulation factor interactions. Our previous results led us to hypothesize that EPA and DHA alter the ability of platelets to ...

  5. The Pattern of Fatty Acids Displaced by EPA and DHA Following 12 Months Supplementation Varies between Blood Cell and Plasma Fractions

    OpenAIRE

    Walker, Celia G.; West, Annette L.; Browning, Lucy M; Jackie Madden; Gambell, Joanna M; Jebb, Susan A.; Calder, Philip C.

    2015-01-01

    Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are increased in plasma lipids and blood cell membranes in response to supplementation. Whilst arachidonic acid (AA) is correspondingly decreased, the effect on other fatty acids (FA) is less well described and there may be site-specific differences. In response to 12 months EPA + DHA supplementation in doses equivalent to 0–4 portions of oily fish/week (1 portion: 3.27 g EPA+DHA) multinomial regression analysis was used to identify...

  6. DHA effect on chemotherapy-induced body weight loss: an exploratory study in a rodent model of mammary tumors.

    Science.gov (United States)

    Hajjaji, Nawale; Couet, Charles; Besson, Pierre; Bougnoux, Philippe

    2012-01-01

    Body weight loss during the course of cancer disease has been associated with poor prognosis. Beside cancer-associated cachexia, weight loss can also result from chemotherapy. This work explored whether a model of mammary tumors in female Sprague Dawley rats could be appropriate to study the effect of doxorubicin on body weight, described weight change in this model, and assessed the effect of DHA on weight during chemotherapy. After tumor induction, rats were randomly assigned to a control or a DHA-enriched diet, and treated with doxorubicin or placebo twice a week for 2.5 wk (n = 6 in each group). Body weight, food intake, and tumor growth were monitored. Neither the induction of tumors nor their initial development impaired body weight gain. No reduction in food intake was observed. Tumor growth was similar between groups from day 1 to day 11. Although doxorubicin induced body weight loss from day 4 compared to placebo (Pweight loss in rats fed the DHA-enriched diet (P = 0.02), indicating that DHA had a protective effect. These results indicate that doxorubicin can induce body weight loss in this model and that a DHA-enriched diet can prevent this effect.

  7. Bad is not involved in DHA-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells

    Science.gov (United States)

    Yu, Huai-na; Lu, Ying-ying; Chen, Tong-sheng

    2011-03-01

    Dihydroartemisinin (DHA), a first-line anti-malarial drug with low toxicity, has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathway, but the molecular mechanisms are not well understood. In this paper, we focus on whether Bad, a BH3-only pro-apoptotic protein, is involved in apoptotic cell death in DHA-treated human lung adenocarcinoma (ASTC-a-1) cells. Confocal fluorescence microscope imaging was used to monitor the temporal and spatial distribution of Bad in single living cells. Our results indicate that Bad is still located in cytoplasm and does not translocate to mitochondria after treatment with DHA for 24 h, while only a small proportion of Bad located in cytoplasm in the STS-treated cells for 6 h. These results show for the first time that Bad is not involved in DHA-induced apoptosis in ASTC-a-1 cells, which could give more evidence for the molecular mechanisms of apoptosis induced by DHA.

  8. Comparison of the effects of EPA and DHA alone or in combination in a murine model of myocardial infarction.

    Science.gov (United States)

    Madingou, Ness; Gilbert, Kim; Tomaro, Leandro; Prud'homme Touchette, Charles; Trudeau, François; Fortin, Samuel; Rousseau, Guy

    2016-08-01

    The aim of this project was to investigate the impact of two dietary omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), alone or in combination, on infarct size. Adult, male Sprague-Dawley rats were fed for 14 days with different omega-3 diets. The animals were subjected to ischemia for 40min followed by reperfusion. Infarct size, Akt (protein kinase B) activation level, caspase-3 activity and mitochondrial permeability transition pore (mPTP) opening were measured. The results indicate that EPA or DHA alone significantly reduced infarct size compared to the other diets. Akt activity was increased in the group fed EPA or DHA alone, whereas no significant activation was observed in the other groups compared to no omega-3 PUFA. DHA alone reduced caspase-3 activity and conferred resistance to mPTP opening. In conclusion, our results demonstrate that EPA and DHA are individually effective in diminishing infarct size in our experimental model while their combination is not. PMID:27499449

  9. Physicochemical properties and antioxidant activity of α-tocopherol loaded nanoliposome's containing DHA and EPA.

    Science.gov (United States)

    Sahari, Mohammad Ali; Moghimi, Hamid Reza; Hadian, Zahra; Barzegar, Mohsen; Mohammadi, Abdorreza

    2017-01-15

    The aim of this study was to prepare α-tocopherol loaded nanoliposomes as carriers of DHA and EPA and to investigate their physicochemical properties, such as peroxide value (PV), volatile compounds (VOCs), particle size, size distribution, zeta potential and morphology of the liposomes. The particle size of liposomes was in the range of 82.4-107.2nm. The highest extent of lipid oxidation was observed at 40°C for 90days, with the lowest PV and propanal levels for a nanoliposome formulation in comparison with the control sample. The zeta potential of the nanoliposomes was decreased during storage. No significant change in the PV and zeta potential of the liposome formulations with α-tocopherol was observed at 4°C after 90days (0.14meq/kg and -43.5mV, respectively). This study demonstrated that incorporation of α-tocopherol into liposomes contributes a significant antioxidant effect on DHA and EPA. PMID:27542462

  10. EPA- and DHA-derived resolvins' actions in inflammatory bowel disease.

    Science.gov (United States)

    Schwanke, Raquel Cristina; Marcon, Rodrigo; Bento, Allisson Freire; Calixto, João B

    2016-08-15

    Inflammatory bowel diseases are chronic diseases divided into two major forms, ulcerative colitis and Crohn's disease, which are both associated with a chronic inflammatory condition of the gastrointestinal tract. Recent studies have shown that the resolution of inflammatory conditions is a biosynthetically active process where new pro-resolution lipid mediators derived from omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), such as E- and D-series resolvins, protectins, and macrophage mediator in resolving inflammation (maresins), have potent anti-inflammatory activity and serve as specialised mediators that play an important role in the resolution of inflammation. Recent studies have also shown the role of resolvins in referred hyperalgesia associated with different inflammatory processes, such as the visceral pain caused by inflammatory bowel disease. There are many reports describing the principal effects of EPA- and DHA-derived mediators in experimental models of inflammatory bowel diseases. This review focuses on the recent studies on the important role played by pro-resolution lipid mediators in controlling the inflammatory process associated with inflammatory bowel diseases. PMID:26325092

  11. 培养海洋微藻Isochrysis galbana生产EPA和DHA%EPA AND DHA PRODUCTIVITY BY MARINE MICROALGA Isochrysis galbana

    Institute of Scientific and Technical Information of China (English)

    戴俊彪; 吴庆余

    1999-01-01

    @@ 某些海洋鱼油是EPA(廿碳五烯酸)和DHA(廿二碳六烯酸)等ω-3多不饱和脂肪酸(ω-3 PUFA)的主要来源.由于鱼油资源有限,且鱼油中ω-3 PUFA的构成和含量随鱼的种类、季节、地理位置等不同而变化,利用海洋鱼油来生产ω-3 PUFA受到了很大的限制.另外,利用鱼油生产的EPA和DHA产品带有无法去除的鱼腥味,大大影响了产品的质量.随着人类对自身健康要求的提高,人们对这两种具有良好生理效果的脂肪酸的需求越来越多,仅仅依靠原有的鱼油资源已无法满足日益扩大的市场需求,开发EPA和DHA新生物资源已成为一个受到诸多学者关心的重要课题[1,2].

  12. EFFECTS OF FISH OIL, DHA OIL AND LECITHIN IN MICROPARTICULATE DIETS ON STRESS TOLERANCE OF LARVAL GILTHEAD SEABREAM (SPARUS AURATA)

    Institute of Scientific and Technical Information of China (English)

    刘镜恪; 王文琪; 李岿然; 雷霁霖

    2002-01-01

    The effects of natural fish oil, DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream (Sparus aurata) were investigated after 15 days feeding trials. The tolerance of larval gilthead seabream to various stress factors such as exposure to air ( lack of dissolved oxygen), changes in water temperature (low) and salinity ( high) were determined. This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for increasing the tolerance of larval gilthead seabream to various stresses, and that microparticulate diet with natural fish oil and palmitic acid (16:0) was more effective than microparticulate diet with DHA oil and soybean lecithin.

  13. Age and sex differences in the incorporation of EPA and DHA into plasma fractions, cells and adipose tissue in humans

    OpenAIRE

    Walker, Celia G.; Browning, Lucy M; Mander, Adrian P; Madden, Jackie; West, Annette L.; Calder, Philip C.; Jebb, Susan A.

    2013-01-01

    The aims of this study were to determine whether age and sex influence both the status and the incorporation of EPA and DHA into blood plasma, cells and tissues. The study was a double-blind, randomised, controlled intervention, providing EPA+DHA equivalent to 0, 1, 2 or 4 portions of oily fish per week, for 12 months. Participants were stratified by age and sex. A linear regression model was used to analyse baseline outcomes, with covariates for age or sex groups, and adjusting for BMI. The ...

  14. Production of Structured Phosphatidylcholine with High Content of DHA/EPA by Immobilized Phospholipase A1-Catalyzed Transesterification

    OpenAIRE

    Xiang Li; Jia-Feng Chen; Bo Yang; Dao-Ming Li; Yong-Hua Wang; Wei-Fei Wang

    2014-01-01

    This paper presents the synthesis of structured phosphatidylcholine (PC) enriched with docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) by transesterification of DHA/EPA-rich ethyl esters with PC using immobilized phospholipsase A1 (PLA1) in solvent-free medium. Firstly, liquid PLA1 was immobilized on resin D380, and it was found that a pH of 5 and a support/PLA1 ratio (w/v) of 1:3 were the best conditions for the adsorption. Secondly, the immobilized PLA1 was used to catalyze trans...

  15. (n-3) Fatty Acids and Cardiovascular Health: Are Effects of EPA and DHA Shared or Complementary?123

    OpenAIRE

    Mozaffarian, Dariush; Wu, Jason H Y

    2012-01-01

    Considerable research supports cardiovascular benefits of consuming omega-3 PUFA, also known as (n-3) PUFA, from fish or fish oil. Whether individual long-chain (n-3) PUFA have shared or complementary effects is not well established. We reviewed evidence for dietary and endogenous sources and cardiovascular effects on biologic pathways, physiologic risk factors, and clinical endpoints of EPA [20:5(n-3)], docosapentaenoic acid [DPA, 22:5(n-3)], and DHA [22:6(n-3)]. DHA requires direct dietary ...

  16. Functions and development status in solid drink of DHA%DHA功能及其在固体饮料中的开发现状

    Institute of Scientific and Technical Information of China (English)

    黎校君; 司华静; 黄巍峰; 李建平; 侯文伟

    2012-01-01

    DHA,docosahexaenoic acid,commonly known as the"Golden Brain",a very important polyunsaturated fatty acids belonging to Omega-3 fatty acid family,plays important functions in human body.With an increasing understanding towards DHA,more and more attention has been paid to DHA,and the demand for DHA has increased greatly.DHA is widely applied in health products and food industry.Recently,the addition of DHA into solid drink has become a new application trend.The function and development status in solid drink of DHA was reviewed.%DHA,二十二碳六烯酸,俗称"脑黄金",是一种非常重要的多不饱和脂肪酸,属于Omega-3脂肪酸家族,对人体有着重要的功能。随着人们对DHA认知度的不断提升,对其重视及需求程度也日益增加。在食品和保健品行业,DHA被广泛应用。近来,将DHA添加于固体饮料中成为一种新的应用趋势。本文综述了DHA功能及其在固体饮料中的开发现状。

  17. DHA derivatives of fish oil as dietary supplements: a nutrition-based drug discovery approach for therapies to prevent metabolic cardiotoxicity

    Science.gov (United States)

    Ma, Yonggang; Lindsey, Merry L.; Halade, Ganesh V.

    2014-01-01

    Introduction During the early 1970s, Danish physicians Jorn Dyerberg and colleagues observed that Greenland Eskimos consuming fatty fishes exhibited low incidences of heart disease. Fish oil is now one of the most commonly consumed dietary supplements. In 2004, concentrated fish oil was approved as a drug by the FDA for the treatment of hyperlipidemia. Fish oil contains two major omega-3 fatty acids: eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). With advancements in lipid concentration and purification techniques, EPA- or DHA-enriched products are now commercially available, and the availability of these components in isolation allows their individual effects to be examined. Newly synthesized derivatives and endogenously discovered metabolites of DHA exhibit therapeutic utility for obesity, metabolic syndrome and cardiovascular disease. Areas covered This review summarizes our current knowledge on the distinct effects of EPA and DHA to prevent metabolic syndrome and reduce cardiotoxicity risk. Since EPA is an integral component of fish oil, we will briefly review EPA effects, but our main theme will be to summarize effects of the DHA derivatives that are available today. We focus on using nutrition-based drug discovery to explore the potential of DHA derivatives for the treatment of obesity, metabolic syndrome and cardiovascular diseases. Expert opinion The safety and efficacy evaluation of DHA derivatives will provide novel biomolecules for the drug discovery arsenal. Novel nutritional-based drug discoveries of DHA derivatives or metabolites may provide realistic and alternative strategies for the treatment of metabolic and cardiovascular disease. PMID:22724444

  18. PENGARUH DOCOSAHEXAENOIC ACID (DHA PADA TUMBUH KEMBANG ANAK BALITA GIZI BURUK YANG DIRAWAT JALAN [The effect of DocosaHexaenoic Acid (DHA on growth and development of outpatient rehabilitation of children under-five with severe malnutrition

    Directory of Open Access Journals (Sweden)

    Astuti Lamid1, , , , dan

    2002-12-01

    Full Text Available The study examined the effect of DocosaHexaenoic Acid (DHA on growth and development of outpatient rehabilitation of children under five with severe malnutrition. Sample was children whose age from 6 to 24 months suffering from severe malnutrition with weight /age index of WHO standard of Z score 0,05.

  19. Determination of EPA-E and DHA-E in Fish Oil Pills by Gas Chromatography%鱼油微丸中EPA-E和DHA-E的含量测定

    Institute of Scientific and Technical Information of China (English)

    朱亚尔; 李士敏

    2001-01-01

    A gas chromatographic method for determination of the ethyl esters of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in fish oil pills was reported. The pills were refluxed with absolute ethanol under N2,n-tetracosane was used as internal standard. The average recovery was 100.5%~105.8%, RSD<3.3%. The linear range of EPA-E and DHA-E were 0.24~3.6 and 0.38~5.7 mg/ml, respectively (r=0.9999).%鱼油微丸与无水乙醇在氮气保护下回流,以正二十四烷为内标物,用气相色谱法测定鱼油微丸中EPA-E和DHA-E的含量,两者的加样回收率在100.5%~105.8%之间,RSD小于3.3%,EPA-E在0.24~3.6 mg/ml,DHA-E在0.38~5.7 mg/ml间线性关系良好,相关系数均为0.9999。本法操作简便,结果准确,重现性好。

  20. Maturation of visual acuity is accelerated in breast-fed term infants fed baby food containing DHA-enriched egg yolk.

    Science.gov (United States)

    Hoffman, Dennis R; Theuer, Richard C; Castañeda, Yolanda S; Wheaton, Dianna H; Bosworth, Rain G; O'Connor, Anna R; Morale, Sarah E; Wiedemann, Lindsey E; Birch, Eileen E

    2004-09-01

    Between 6 and 12 mo of age, blood levels of the (n-3) long-chain PUFA, docosahexaenoic acid (DHA), in breast-fed infants typically decrease due to diminished maternal DHA stores and the introduction of DHA-poor solid foods displacing human milk as the primary source of nutrition. Thus, we utilized a randomized, clinical trial format to evaluate the effect of supplemental DHA in solid foods on visual development of breast-fed infants with the primary outcome, sweep visual-evoked potential (VEP) acuity, as an index for maturation of the retina and visual cortex. At 6 mo of age, breast-fed infants were randomly assigned to receive 1 jar (113 g)/d of baby food containing egg yolk enriched with DHA (115 mg DHA/100 g food; n = 25) or control baby food (0 mg DHA; n = 26). Gravimetric measures were used to estimate the supplemental DHA intake which was 83 mg DHA/d in the supplemented group and 0 mg/d in controls. Although many infants in both groups continued to breast-feed for a mean of 9 mo, RBC DHA levels decreased significantly between 6 and 12 mo (from 3.8 to 3.0 g/100 g total fatty acids) in control infants, whereas RBC DHA levels increased by 34% from 4.1 to 5.5 g/100 g by 12 mo in supplemented infants. VEP acuity at 6 mo was 0.49 logMAR (minimum angle of resolution) and improved to 0.29 logMAR by 12 mo in controls. In DHA-supplemented infants, VEP acuity was 0.48 logMAR at 6 mo and matured to 0.14 logMAR at 12 mo (1.5 lines on the eye chart better than controls). At 12 mo, the difference corresponded to 1.5 lines on the eye chart. RBC DHA levels and VEP acuity at 12 mo were correlated (r = -0.50; P = 0.0002), supporting the need of an adequate dietary supply of DHA throughout 1 y of life for neural development. PMID:15333721

  1. Apports en DHA (acide gras oméga-3 par les poissons et les fruits de mer consommés en France

    Directory of Open Access Journals (Sweden)

    Bourre Jean-Marie

    2007-01-01

    Full Text Available This present work measures the contributions of seafood (finfish and shellfish, wild and farmed to the intakes of DHA in France, year 2005, and consequently to the French recommended daily intakes (RDA of DHA. For this purpose, we measured the concentrations of DHA in each individual seafood by analysis of many published data. We also determined the exact consumption of the main products of seafood (fishing and farming in France (year 2005 using the modified method of the dietary intake measurement defined by the FAO. For year 2005, the 34 species of finfish and shellfish whose DHA contents are known account for 150% of the RDA of this omega-3 fatty acid. Taking all the species individually and extrapolating to include those whose DHA contents are not known, gives 156% of the RDA. The 10 greatest contributors to the DHA intake in the present diet are: salmon (47.6 mg/day/person, sardine (28.4, tuna (20.5, mackerel (15.7, herring (12.4, Alaskan pollock (8.9, cod (6.9, trout (6.5, hake (4.7, saithe (4.6. The 5 species of oily fish (salmon, sardine, tuna, mackerel and herring provide 63% of the DHA intake, and thus approx 95% of the French RDA. Besides DHA, seafood contains low amounts of ALA, but it can very high in farmed fish fed on rapeseed or linseed products. Omega-3 fatty acids (including EPA and DHA have an important role in human diet, both for prevention and the therapy of different pathologies. Data from many epidemiological studies has shown an inverse association between fish consumption, generally oily fish, and reductions in certain diseases. Although number of people do not lack dietary DHA, others, mainly pregnant women and nursing mothers and those people whose life styles or socio-economic positions restrict their seafood intakes, would benefit greatly from an increased intake of this omega-3 fatty acid.

  2. Omega-3 DHA and EPA for cognition, behavior, and mood: clinical findings and structural-functional synergies with cell membrane phospholipids.

    Science.gov (United States)

    Kidd, Parris M

    2007-09-01

    The omega-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are orthomolecular, conditionally essential nutrients that enhance quality of life and lower the risk of premature death. They function exclusively via cell membranes, in which they are anchored by phospholipid molecules. DHA is proven essential to pre- and postnatal brain development, whereas EPA seems more influential on behavior and mood. Both DHA and EPA generate neuroprotective metabolites. In double-blind, randomized, controlled trials, DHA and EPA combinations have been shown to benefit attention deficit/hyperactivity disorder (AD/HD), autism, dyspraxia, dyslexia, and aggression. For the affective disorders, meta-analyses confirm benefits in major depressive disorder (MDD) and bipolar disorder, with promising results in schizophrenia and initial benefit for borderline personality disorder. Accelerated cognitive decline and mild cognitive impairment (MCI) correlate with lowered tissue levels of DHA/EPA, and supplementation has improved cognitive function. Huntington disease has responded to EPA. Omega-3 phospholipid supplements that combine DHA/EPA and phospholipids into the same molecule have shown marked promise in early clinical trials. Phosphatidylserine with DHA/EPA attached (Omega-3 PS) has been shown to alleviate AD/HD symptoms. Krill omega-3 phospholipids, containing mostly phosphatidylcholine (PC) with DHA/EPA attached, markedly outperformed conventional fish oil DHA/EPA triglycerides in double-blind trials for premenstrual syndrome/dysmenorrhea and for normalizing blood lipid profiles. Krill omega-3 phospholipids demonstrated anti-inflammatory activity, lowering C-reactive protein (CRP) levels in a double-blind trial. Utilizing DHA and EPA together with phospholipids and membrane antioxidants to achieve a triple cell membrane synergy may further diversify their currently wide range of clinical applications. PMID:18072818

  3. Brain docosahexaenoic acid (DHA levels of young rats are related to alpha-linolenic acid (ALA levels and fat matrix of the diet: impact of dairy fat*

    Directory of Open Access Journals (Sweden)

    Delplanque Bernadette

    2011-11-01

    Full Text Available Dososahexaenoate (DHA is highly concentrated in mammalian nervous and visual systems and its deficiency during gestation, lactation and early life, could have dramatic impacts on brain functions and mental health. Achieving an appropriate DHA status in the neonatal brain is an important goal of neonatal nutrition. We evaluated how a-linolenic acid (ALA provided by different dietary fat matrices improved DHA content in the brains of both young male and female rats. Young rats born from dams fed during gestation and lactation with a low ALA diet (0.4% of fatty acids were subjected for 6 weeks after weaning to an anhydrous dairy fat blend-based diet that provided 1.5% ALA or to a palm oil blend-based diet that provided the same ALA level: either 1.5% ALA or 1.5% ALA and 0.12% DHA with 0.4% arachidonic acid (ARA. With each diet the n-6/ n-3 ratio was similar (10 to follow the values generally recommended for infant formula. Fatty acids analysis in whole brain showed that 1.5% ALA dairy fat blend was superior to both 1.5% ALA palm-oil blends, supplemented or not with dietary DHA, for increasing brain DHA. Females compared to males had significantly higher brain DHA with the 1.5% ALA palm-blend diet, but the dietary supplementation with DHA smoothed the differences by a specific increase of males DHA brain. In conclusion, dairy fat blend enriched with ALA appear to be an interesting strategy for achieving optimal DHA levels in the brain of post-weaning rats. Inclusion of dairy fat in infant formulas should be reconsidered.

  4. Odour characteristics of seafood flavour formulations produced with fish by-products incorporating EPA, DHA and fish oil.

    Science.gov (United States)

    Peinado, I; Miles, W; Koutsidis, G

    2016-12-01

    Thermal degradation of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids was investigated. As a novelty, EPA, DHA or fish oil (FO) were incorporated as ω-fatty acid sources into model systems containing fish powder produced via Maillard reactions. Aroma composition of the resulting products was determined and complemented with sensory evaluation. Heating of the oils led to a fast decrease of both, EPA and DHA, and to the development of characteristic volatile compounds including hexanal, 2,4-heptadienal and 4-heptenal, the most abundant being (E,E)-2,4-heptadienal (132±44-329±122μmol/g). EPA and DHA addition to the model systems increased the concentration of these characteristic volatile compounds. However, it did not have a considerable impact on the development of characteristic Maillard reaction products, such as pyrazines and some aldehydes. Finally, the results of the sensory evaluation illustrated that panellists would chose samples fortified with FO as the ones with a more pleasant aroma. PMID:27374575

  5. Effect of DHA and CoenzymeQ10 Against Aβ- and Zinc-Induced Mitochondrial Dysfunction in Human Neuronal Cells

    Directory of Open Access Journals (Sweden)

    Nadia Sadli

    2013-07-01

    Full Text Available Background: Beta-amyloid (Aβ protein is a key factor in the pathogenesis of Alzheimer's disease (AD and it has been reported that mitochondria is involved in the biochemical pathway by which Aβ can lead to neuronal dysfunction. Coenzyme Q10 (CoQ10 is an essential cofactor involved in the mitochondrial electron transport chain and has been suggested as a potential therapeutic agent in AD. Zinc toxicity also affects cellular energy production by decreasing oxygen consumption rate (OCR and ATP turnover in human neuronal cells, which can be restored by the neuroprotective effect of docosahexaenoic acid (DHA. Method: In the present study, using Seahorse XF-24 Metabolic Flux Analysis we investigated the effect of DHA and CoQ10 alone and in combination against Aβ- and zinc-mediated changes in the mitochondrial function of M17 neuroblastoma cell line. Results: Here, we observed that DHA is specifically neuroprotective against zinc-triggered mitochondrial dysfunction, but does not directly affect Aβ neurotoxicity. CoQ10 has shown to be protective against both Aβ- and zinc-induced alterations in mitochondrial function. Conclusion: Our results indicate that DHA and CoQ10 may be useful for the prevention, treatment and management of neurodegenerative diseases such as AD.

  6. Production of Structured Phosphatidylcholine with High Content of DHA/EPA by Immobilized Phospholipase A1-Catalyzed Transesterification

    Directory of Open Access Journals (Sweden)

    Xiang Li

    2014-08-01

    Full Text Available This paper presents the synthesis of structured phosphatidylcholine (PC enriched with docosahexaenoic acid (DHA and eicosapentaenoic acid (EPA by transesterification of DHA/EPA-rich ethyl esters with PC using immobilized phospholipsase A1 (PLA1 in solvent-free medium. Firstly, liquid PLA1 was immobilized on resin D380, and it was found that a pH of 5 and a support/PLA1 ratio (w/v of 1:3 were the best conditions for the adsorption. Secondly, the immobilized PLA1 was used to catalyze transesterification of PC and DHA/EPA-rich ethyl esters. The maximal incorporation of DHA and EPA achieved was 30.7% for 24 h of reaction at 55 °C using a substrate mass ratio (PC/ethyl esters of 1:6, an immobilized PLA1 loading of 15% and water dosage of 1.25%. Then the reaction mixture was analyzed by 31P nuclear magnetic resonance (NMR. The composition of reaction product included 16.5% PC, 26.3% 2-diacyl-sn-glycero-3-lysophosphatidylcholine (1-LPC, 31.4% 1-diacyl-sn-glycero-3-lysophosphatidylcholine (2-LPC, and 25.8% sn-glycerol-3-phosphatidylcholine (GPC.

  7. DHA- RICH FISH OIL IMPROVES COMPLEX REACTION TIME IN FEMALE ELITE SOCCER PLAYERS

    Directory of Open Access Journals (Sweden)

    José F. Guzmán

    2011-06-01

    Full Text Available Omega-3 fatty acids (n-3 has shown to improve neuromotor function. This study examined the effects of docosahexaenoic acid (DHA on complex reaction time, precision and efficiency, in female elite soccer players. 24 players from two Spanish female soccer Super League teams were randomly selected and assigned to two experimental groups, then administered, in a double-blind manner, 3.5 g·day-1 of either DHA-rich fish oil (FO =12 or olive oil (OO = 12 over 4 weeks of training. Two measurements (pre- and post-treatment of complex reaction time and precision were taken. Participants had to press different buttons and pedals with left and right hands and feet, or stop responding, according to visual and auditory stimuli. Multivariate analysis of variance displayed an interaction between supplement administration (pre/post and experimental group (FO/OO on complex reaction time (FO pre = 0.713 ± 0.142 ms, FO post = 0.623 ± 0.109 ms, OO pre = 0.682 ± 1.132 ms, OO post = 0.715 ± 0.159 ms; p = 0.004 and efficiency (FO pre = 40.88 ± 17.41, FO post = 57.12 ± 11.05, OO pre = 49.52 ± 14.63, OO post = 49. 50 ± 11.01; p = 0.003. It was concluded that after 4 weeks of supplementation with FO, there was a significant improvement in the neuromotor function of female elite soccer players

  8. Predicting the effect of maternal docosahexaenoic acid (DHA) supplementation to reduce early preterm birth in Australia and the United States using results of within country randomized controlled trials.

    Science.gov (United States)

    Yelland, L N; Gajewski, B J; Colombo, J; Gibson, R A; Makrides, M; Carlson, S E

    2016-09-01

    The DHA to Optimize Mother Infant Outcome (DOMInO) and Kansas DHA Outcomes Study (KUDOS) were randomized controlled trials that supplemented mothers with 800 and 600mg DHA/day, respectively, or a placebo during pregnancy. DOMInO was conducted in Australia and KUDOS in the United States. Both trials found an unanticipated and statistically significant reduction in early preterm birth (ePTB; i.e., birth before 34 weeks gestation). However, in each trial, the number of ePTBs were small. We used a novel Bayesian approach to estimate statistically derived low, moderate or high risk for ePTB, and to test for differences between the DHA and placebo groups. In both trials, the model predicted DHA would significantly reduce the expected proportion of deliveries in the high risk group under the trial conditions of the parent studies. Among the next 300,000 births in Australia we estimated that 1112 ePTB (95% credible interval 51-2189) could be avoided by providing DHA. And in the USA we estimated that 106,030 ePTB (95% credible interval 6400 to 175,700) could be avoided with DHA.

  9. Predicting the effect of maternal docosahexaenoic acid (DHA) supplementation to reduce early preterm birth in Australia and the United States using results of within country randomized controlled trials.

    Science.gov (United States)

    Yelland, L N; Gajewski, B J; Colombo, J; Gibson, R A; Makrides, M; Carlson, S E

    2016-09-01

    The DHA to Optimize Mother Infant Outcome (DOMInO) and Kansas DHA Outcomes Study (KUDOS) were randomized controlled trials that supplemented mothers with 800 and 600mg DHA/day, respectively, or a placebo during pregnancy. DOMInO was conducted in Australia and KUDOS in the United States. Both trials found an unanticipated and statistically significant reduction in early preterm birth (ePTB; i.e., birth before 34 weeks gestation). However, in each trial, the number of ePTBs were small. We used a novel Bayesian approach to estimate statistically derived low, moderate or high risk for ePTB, and to test for differences between the DHA and placebo groups. In both trials, the model predicted DHA would significantly reduce the expected proportion of deliveries in the high risk group under the trial conditions of the parent studies. Among the next 300,000 births in Australia we estimated that 1112 ePTB (95% credible interval 51-2189) could be avoided by providing DHA. And in the USA we estimated that 106,030 ePTB (95% credible interval 6400 to 175,700) could be avoided with DHA. PMID:27637340

  10. Lipid structure does not modify incorporation of EPA and DHA into blood lipids in healthy adults: a randomised-controlled trial.

    Science.gov (United States)

    West, Annette L; Burdge, Graham C; Calder, Philip C

    2016-09-01

    Dietary supplementation is an effective means to improve EPA and DHA status. However, it is unclear whether lipid structure affects EPA+DHA bioavailability. We determined the effect of consuming different EPA and DHA lipid structures on their concentrations in blood during the postprandial period and during dietary supplementation compared with unmodified fish oil TAG (uTAG). In a postprandial cross-over study, healthy men (n 9) consumed in random order test meals containing 1·1 g EPA+0·37 g DHA as either uTAG, re-esterified TAG, free fatty acids (FFA) or ethyl esters (EE). In a parallel design supplementation study, healthy men and women (n 10/sex per supplement) consumed one supplement type for 12 weeks. Fatty acid composition was determined by GC. EPA incorporation over 6 h into TAG or phosphatidylcholine (PC) did not differ between lipid structures. EPA enrichment in NEFA was lower from EE than from uTAG (P=0·01). Plasma TAG, PC or NEFA DHA incorporation did not differ between lipid structures. Lipid structure did not affect TAG or NEFA EPA incorporation and PC or NEFA DHA incorporation following dietary supplementation. Plasma TAG peak DHA incorporation was greater (P=0·02) and time to peak shorter (P=0·02) from FFA than from uTAG in men. In both studies, the order of EPA and DHA incorporation was PC>TAG>NEFA. In conclusion, EPA and DHA lipid structure may not be an important consideration in dietary interventions. PMID:27424661

  11. ANALISIS DEL CO-TRATAMIENTO PASIVO DE AGUAS RESIDUALES MUNICIPALES Y DRENAJE ACIDO DE MINAS EN CERRO RICO DE POTOSÍ, BOLIVIA

    Directory of Open Access Journals (Sweden)

    William H.J. Strosnider

    2015-01-01

    Full Text Available El drenaje acido de minas (DAM es un problema a nivel mundial. El co-tratamiento pasivo de DAM con aguas residuales municipales (ARM es una manera efectiva y de bajo costo que utiliza los nutrientes de ARM para tratar las concentraciones de metales y sulfato en DAM. Cerro Rico de Potosí, Bolivia es una de las grandes ciudades mineras en el mundo que tiene muchos problemas con el DAM. El objetivo de este estudio fue determinar velocidades de reacción de Al, Fe, Mn, Zn, y otros metales en DAM provenientes de un flujo de agua en Cerro Rico usando un sistema reactor de tres etapas. El DAM tenía un pH de 3.58, acidez de 1080 mg/L equivalente a CaCO3 conteniendo concentraciones de 12, 68, 17, y 550 mg/L de Al, Fe, Mn y Zn respectivamente. Las velocidades de reacción de Al, Fe, Mn y Zn fueron de 1.43, 2.09, 0.01 y 0.10 d-1, respectivamente.

  12. ESTUDIO DE LA ADSORCIÓN DE PROTEÍNAS SOBRE SUPERFICIES DE ACIDO POLILÁCTICO MEDIANTE TÉCNICAS GRAVIMÉTRICAS Y ELECTROQUÍMICAS

    Directory of Open Access Journals (Sweden)

    HUGO ARMANDO ESTUPIÑAN DURAN

    2011-01-01

    Full Text Available Metodos electroquimicos fueron utilizados para promover y evaluar la adsorcion orientada de albumina y colageno sobre superficies polimericas de acido polilactico (PLA mediante la aplicacion de un sobrepotencial anodico en un rango de 0 V a 0,45 V. La adsorcion fueestudiada usando mediciones simultaneas de espectroscopia de impedancia electroquimica (EIE y microbalanza de cristal de cuarzo (QCM, en un rango de temperaturas de 25°C a 45°C, con variaciones en proteina entre 5 y 20 ¿Êg/ml en solucion buffer de fosfato de pH 7,4. Con el aumento de la concentracion de proteinas en el electrolito, disminuye la resistencia a la transferencia de carga, exhibiendo asi una mayor adsorcion sobre la superficie, ademas de aumentar la magnitud de los cambios de masa. Se mejoro la hidrofilicidad del recubrimiento, mostrando una mayor afinidad con las soluciones de colageno y por lo tanto un aumento en la biocompatibilidad debido a la activacion superficial.

  13. Hydrogen sulfide corrosion of weld regions in API X52 steel; Corrosion por acido sulfhidrico de las regiones de soldadura en acero API X52

    Energy Technology Data Exchange (ETDEWEB)

    Arenas-Martinez, L.F [Universidad Autonoma de Coahuila, Coahuila (Mexico)]. E-mail: fernando.arenas@uadec.edu.mx; Garcia-Cerecero, G. [Corporacion Mexicana de Investigacion en Materiales S.A. de C.V., Saltillo, Coahuila (Mexico)]. E-mail: ggarcia@comimsa.com

    2012-10-15

    The corrosion behavior of gas metal arc welding (GMAW) regions has been studied using potentiodynamic polarization and polarization resistance (LPR) techniques. Experiments were conducted in hydrogen sulfide (H{sub 2}S)-containing brine and in H{sub 2}S-free brine. Welds were made on API 5L X52 steel. Due to differences in their microstructure, chemical composition and residual stress level, weld regions exhibited different responses under H{sub 2}S corrosion. Base metal exhibited the highest corrosion rate (CR) and the most cathodic corrosion potential. [Spanish] Se estudio el comportamiento ante la corrosion de las regiones de soldadura de un cordon realizado por arco metalico con gas (GMAW) sobre un acero grado API X52 mediante las tecnicas de polarizacion potencio dinamica y resistencia a la polarizacion (LPR). Los experimentos se realizaron utilizando salmuera con 300 ppm de acido sulfhidrico (H{sub 2}S) y salmuera libre de H{sub 2}S como electrolitos. Debido a las diferencias en su microestructura, composicion quimica y el nivel de esfuerzos residuales, las regiones de soldadura mostraron diferentes respuestas a la corrosion por H{sub 2}S. El metal base exhibio la velocidad de corrosion (VC) mas alta y el potencial de corrosion mas catodico.

  14. ELOVL2基因多态性与乳母乳汁 DHA 水平的关联性分析%Association analysis on polymorphisms of ELOVL2 gene and DHA level in breast milk of lactating mothers

    Institute of Scientific and Technical Information of China (English)

    李响; 丁珍; 吴义霞; 陈雪妍; 刘国良; 谢林

    2015-01-01

    目的:探讨 ELOVL 脂肪酸延长酶基因2(ELOVL2)rs2281591和 rs3798713位点与乳母乳汁二十二碳六烯酸(DHA)水平的关系,阐明 ELOVL2基因多态性对乳汁 DHA 水平的影响。方法:选取健康产妇209人,在产后第22~25天签署知情同意书并进行三天二十四小时膳食回顾调查,收取乳汁20 mL,应用气相色谱法检测乳汁 DHA 水平,并提取乳汁基因组 DNA,应用 Sequenom Mass Array 系统检测 ELOVL2基因2个单核苷酸多态性(SNP)位点基因型,采用 UNPHASED 3.012遗传学软件进行多位点单倍型与乳汁 DHA 水平的数量性状分析。结果:ELOVL2基因 rs2281591和 rs3798713位点基因型频数分布均符合 Hardy-Weinberg 平衡定律(P >0.05);不同基因型的乳母膳食脂肪酸摄入量和乳汁 DHA 水平差异均无统计学意义(P >0.05);不同 rs3798713(CG)-rs2281591(AG)单倍型的乳母乳汁 DHA 水平比较差异无统计学意义(χ2=3.422,df =5,P =0.635)。结论:ELOVL2基因 rs3798713和 rs2281591位点及其组成的单倍型与乳母乳汁 DHA 水平无关联。%Objective To investigate the association between rs2281951 and rs3798753 in ELOVL fatty acid elongase2 (ELOVL2 gene)and the docosahexenoic acid (DHA)level in breast milk,and to clarify the influence of the polymorphisms of ELOVL2 gene in the DHA level of breast milk.Methods 209 healthy maternals were selected and signed the consent form and completed the 3-day 24-hour dietary recall questionaire on one day during the 22nd-the 25th day after partum,and 20 mL breast milk was collected.The DHA level in breast milk was detected with gas chromatography.The milk DNA was extracted and two single nucleotide polymorphisms (SNPs) of ELOVL2 gene were detected by Sequenom Mass Array System. UNPHASED 3.012 genetics software was adopted to analyze the quantitative trait of haplotype and the DHA level in breast milk.Results The distribution of genotypic

  15. Physiological function and development of extraction method of EPA and DHA%EPA、DHA的生理功能及提取方法的研究进展

    Institute of Scientific and Technical Information of China (English)

    石雨; 田媛; 李磊; 李国巍; 阚侃

    2014-01-01

    近年来,EPA和DHA已经成为人们关注的热点。本文叙述了EPA和DHA的生理功能和正确摄入的重要性,提出采用不同传统方法相结合的方式来高纯度分离提取EPA和DHA。%EPA and DHA have been in focus in recent years. This paper describes the physiological function of EPA and DHA,notes the importance of proper intake,and combines different isolation principles to extract high-purity EPA and DHA.

  16. Effect of consuming novel foods consisting high oleic canola oil, barley β-glucan, and DHA on cardiovascular disease risk in humans: the CONFIDENCE (Canola Oil and Fibre with DHA Enhanced) study – protocol for a randomized controlled trial

    OpenAIRE

    Ramprasath, Vanu R; Thandapilly, Sijo J.; Yang, Shuo; Abraham, Anjalika; Jones, Peter J.H.; Ames, Nancy

    2015-01-01

    Background Metabolic syndrome (MetS) has been identified as a major contributor to the development of cardiovascular disease (CVD). Current recommendations for dietary management of people with MetS involve quantitative and qualitative modifications of food intake, such as high consumption of vegetables, fruits, and whole grain foods. The results from our previous human trials revealed the potential of the dietary components high-oleic acid canola oil (HOCO)-docosahexaenoic acid (DHA) and hig...

  17. Purification of DHA algal oil by molecular distillation%分子蒸馏法纯化DHA藻油

    Institute of Scientific and Technical Information of China (English)

    梁井瑞; 胡耀池; 陈园力; 蒋露; 张红漫

    2012-01-01

    采用分子蒸馏对脱色DHA藻油进行纯化,以酸值、不皂化物、DHA含量等为分析指标,考察了不同的蒸馏温度、进料速率、刮膜器转速对DHA藻油分离除杂效果的影响.结果显示,在进料预热温度30℃,冷凝水25℃,系统操作压力0.3Pa条件下,蒸馏温度240℃,进料速率2.0 mL/min,刮膜器转速150 r/min为最佳工艺参数.采用气相色谱分析,分子蒸馏纯化后DHA含量46.07%,高于传统脱臭工艺的38.41%.%The purification of DHA algal oil by molecular distillation was studied. The effect of distillation temperature,feeding rate and scraper speed on DHA algal oil separation was taken into consideration by analysing the indicators such as acid value,non - saponification content and DHA content were measured. The results showed that the content of DHA was up to 46. 07% under the conditions: system operation pressure 0.3 Pa,distillation temperature 2401,feeding rate 2.0 miymin and scraper speed 150 r/min.

  18. Neuroprostanes, produced by free-radical mediated peroxidation of DHA, inhibit the inflammatory response of human macrophages.

    Science.gov (United States)

    Gladine, Cécile; Laurie, Joumard-Cubizolles; Giulia, Chinetti; Dominique, Bayle; Corinne, Copin; Nathalie, Hennuyer; Bart, Staels; Giuseppe, Zanoni; Alessio, Porta; Jean-Marie, Galano; Camille, Oger; Thierry, Durand

    2014-10-01

    The anti-inflammatory properties of DHA have been largely demonstrated in vitro and in vivo but research gaps remain regarding the contribution of the oxygenated metabolites. Among them, we are focusing on prostaglandin-like molecules termed Neuroprostanes (NeuroPs) which are produced through free-radical-mediated peroxidation of DHA. We hypothesized that these specific molecules which are highly reactive and produced in abundance during oxidative stress and inflammation could contribute to the anti-inflammatory properties of DHA. Human peripheral blood mononuclear cells were isolated from healthy donors by Ficoll density gradient centrifugation. Monocytes were differentiated into resting macrophages (RM) for 6 days (37°C, 5% CO2). RM were exposed to 2 different types of NeuroPs (i.e. 14-A4-NeuroP and 4-F4t-NeuroP, 10µM) or ethanol (vehicle 0.15%) during 30min. Then LPS (100ng/mL) was added for 6hours to induced inflammatory response. Both types of NeuroPs (14-A4-NeuroP and 4-F4t-NeuroP) significantly decreased the mRNA levels of IL-6 (-49% and -26% respectively) and MCP-1 (-55% and -24% respectively). Secretion of TNFα and MCP-1 was also reduced when RM were exposed to 14-A4-NeuroP (-10%, ns and -34%, pinhibition of IkBa phosphorylation. Finally, cotransfection of luciferase reporter vector with hPPARg expression vector performed on Cos-7 cells suggests that NeuroPs probably act independently of PPARg. In conclusion, these results suggest that the anti-inflammatory properties of DHA could be mediated, at least in part, by NeuroPs which corroborates the importance of oxidative stress in cell signaling. PMID:26461294

  19. Scientific Opinion on the Tolerable Upper Intake Level of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA)

    OpenAIRE

    EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA)

    2012-01-01

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to deliver a scientific opinion on the Tolerable Upper Intake Level (UL) of the n-3 LCPUFAs eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA). Available data are insufficient to establish a UL for n-3 LCPUFA (individually or combined) for any population group. At observed intake levels, consumption of n-3 LCPUFA has not been associa...

  20. Unraveling the dha cluster in Citrobacter werkmanii: comparative genomic analysis of bacterial 1,3-propanediol biosynthesis clusters.

    Science.gov (United States)

    Maervoet, Veerle E T; De Maeseneire, Sofie L; Soetaert, Wim K; De Mey, Marjan

    2014-04-01

    In natural 1,3-propanediol (PDO) producing microorganisms such as Klebsiella pneumoniae, Citrobacter freundii and Clostridium sp., the genes coding for PDO producing enzymes are grouped in a dha cluster. This article describes the dha cluster of a novel candidate for PDO production, Citrobacter werkmanii DSM17579 and compares the cluster to the currently known PDO clusters of Enterobacteriaceae and Clostridiaceae. Moreover, we attribute a putative function to two previously unannotated ORFs, OrfW and OrfY, both in C. freundii and in C. werkmanii: both proteins might form a complex and support the glycerol dehydratase by converting cob(I)alamin to the glycerol dehydratase cofactor coenzyme B12. Unraveling this biosynthesis cluster revealed high homology between the deduced amino acid sequence of the open reading frames of C. werkmanii DSM17579 and those of C. freundii DSM30040 and K. pneumoniae MGH78578, i.e., 96 and 87.5 % identity, respectively. On the other hand, major differences between the clusters have also been discovered. For example, only one dihydroxyacetone kinase (DHAK) is present in the dha cluster of C. werkmanii DSM17579, while two DHAK enzymes are present in the cluster of K. pneumoniae MGH78578 and Clostridium butyricum VPI1718.

  1. Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia 1,3-propanediol-producing strains

    Directory of Open Access Journals (Sweden)

    Diana Milena Quilaguy-Ayure

    2010-04-01

    Full Text Available To analyze the dhaT gene, one of the genes responsible for the 1,3-propanediol (1,3-PD production, in two native Clostridiumstrains. Materials and methods: The dhaT gene was amplified by Polimerase Chain Reaction with specific primers designed fromClostridium butyricum VPI1718 operon. Bioinformatics tools like BLASTN, ORF finder, BLASTP and ClustalW were used to determinethe identity of the sequence and to assign a function. Results: DNA amplification products were obtained from Colombian Clostridium sp.native strains (IBUN 13A and IBUN 158B and the Clostridium butyricum DSM 2478 strain, which were sequenced. According to thebioinformatics analysis of the above sequences, a high degree of similarity was found with the dhaT gene of different bacterial species. Thehighest percentage of identity was obtained with the Clostridium butyricum VPI 1718 strain. Conclusion: knowledge of the physicalstructure of the 1,3-PD operon in native strains opens the way for developing genetic and metabolic engineering strategies for improvingprocesses productivity.

  2. Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease.

    Science.gov (United States)

    Bascoul-Colombo, Cécile; Guschina, Irina A; Maskrey, Benjamin H; Good, Mark; O'Donnell, Valerie B; Harwood, John L

    2016-06-01

    Alzheimer's disease (AD) is of major concern in ageing populations and we have used the Tg2576 mouse model to understand connections between brain lipids and amyloid pathology. Because dietary docosahexaenoic acid (DHA) has been identified as beneficial, we compared mice fed with a DHA-supplemented diet to those on a nutritionally-sufficient diet. Major phospholipids from cortex, hippocampus and cerebellum were separated and analysed. Each phosphoglyceride had a characteristic fatty acid composition which was similar in cortex and hippocampus but different in the cerebellum. The biggest changes on DHA-supplementation were within ethanolamine phospholipids which, together with phosphatidylserine, had the highest proportions of DHA. Reciprocal alterations in DHA and arachidonate were found. The main diet-induced alterations were found in ethanolamine phospholipids, (and included their ether derivatives), as were the changes observed due to genotype. Tg mice appeared more sensitive to diet with generally lower DHA percentages when on the standard diet and higher relative proportions of DHA when the diet was supplemented. All four major phosphoglycerides analysed showed age-dependent decreases in polyunsaturated fatty acid contents. These data provide, for the first time, a detailed evaluation of phospholipids in different brain areas previously shown to be relevant to behaviour in the Tg2576 mouse model for AD. The lipid changes observed with genotype are consistent with the subtle alterations found in AD patients, especially for the ethanolamine phospholipid molecular species. They also emphasise the contrasting changes in fatty acid content induced by DHA supplementation within individual phospholipid classes. PMID:26968097

  3. Long-chain omega-3 fatty acids and the brain: A review of the independent and shared effects of EPA, DPA and DHA

    Directory of Open Access Journals (Sweden)

    Simon eDyall

    2015-04-01

    Full Text Available Omega-3 polyunsaturated fatty acids (PUFAs exhibit neuroprotective properties and represent a potential treatment for a variety of neurodegenerative and neurological disorders. However, traditionally there has been a lack of discrimination between the different omega-3 PUFAs and effects have been broadly accredited to series as a whole. Evidence for unique effects of eicosapentaenoic acid (EPA, docosahexaenoic acid (DHA and more recently docosapentaenoic acid (DPA is growing. For example, beneficial effects in mood disorders have more consistently been reported in clinical trials using EPA; whereas, with neurodegenerative conditions such as Alzheimer’s disease, the focus has been on DHA. DHA is quantitatively the most important omega-3 PUFA in the brain, and consequently the most studied, whereas the availability of high purity DPA preparations has been extremely limited until recently, limiting research into its effects. However, there is now a growing body of evidence indicating both independent and shared effects of EPA, DPA and DHA. The purpose of this review is to highlight how a detailed understanding of these effects is essential to improving understanding of their therapeutic potential. The review begins with an overview of omega-3 PUFA biochemistry and metabolism, with particular focus on the central nervous system, where DHA has unique and indispensable roles in neuronal membranes with levels preserved by multiple mechanisms. This is followed by a review of the different enzyme-derived anti-inflammatory mediators produced from EPA, DPA and DHA. Lastly, the relative protective effects of EPA, DPA and DHA in normal brain aging and the most common neurodegenerative disorders are discussed. With a greater understanding of the individual roles of EPA, DPA and DHA in brain health and repair it is hoped that appropriate dietary recommendations can be established and therapeutic interventions can be more targeted and refined.

  4. EPA/DHA and vitamin A supplementation improves spatial memory and alleviates the age-related decrease in hippocampal RXRγ and kinase expression in rats

    OpenAIRE

    Létondor, Anne; Buaud, Benjamin; Vaysse, Carole; Richard, Emmanuel; Layé, Sophie; Pallet, Véronique; Alfos, Serge

    2016-01-01

    Studies suggest that eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and vitamin A are critical to delay aged-related cognitive decline. These nutrients regulate gene expression in the brain by binding to nuclear receptors such as the retinoid X receptors (RXRs) and the retinoic acid receptors (RARs). Moreover, EPA/DHA and retinoids activate notably kinase signaling pathways such as AKT or MAPK, which includes ERK1/2. This suggests that these nutrients may modulate brain function in ...

  5. Effect of α-linolenic acid and DHA intake on lipogenesis and gene expression involved in fatty acid metabolism in growing-finishing pigs.

    Science.gov (United States)

    De Tonnac, A; Labussière, E; Vincent, A; Mourot, J

    2016-07-01

    The regulation of lipogenesis mechanisms related to consumption of n-3 PUFA is poorly understood. The aim of the present study was to find out whether α-linolenic acid (ALA) or DHA uptake can have an effect on activities and gene expressions of enzymes involved in lipid metabolism in the liver, subcutaneous adipose tissue and longissimus dorsi (LD) muscle of growing-finishing pigs. Six groups of ten pigs received one of six experimental diets supplemented with rapeseed oil in the control diet, extruded linseed, microalgae or a mixture of both to implement different levels of ALA and DHA with the same content in total n-3. Results were analysed for linear and quadratic effects of DHA intake. The results showed that activities of malic enzyme (ME) and fatty acid synthase (FAS) decreased linearly in the liver with dietary DHA. Although the expression of the genes of these enzymes and their activities were poorly correlated, ME and FAS expressions also decreased linearly with DHA intake. The intake of DHA down-regulates the expressions of other genes involved in fatty acid (FA) metabolism in some tissues of pigs, such as fatty acid desaturase 2 and sterol-regulatory element binding transcription factor 1 in the liver and 2,4-dienoyl CoA reductase 2 in the LD muscle. FA oxidation in the LD muscle and FA synthesis decreased in the liver with increasing amount of dietary DHA, whereas a retroconversion of DHA into EPA seems to be set up in this last tissue. PMID:27181335

  6. Evaluation of suppressive and pro-resolving effects of EPA and DHA in human primary monocytes and T-helper cells[S

    OpenAIRE

    Jaudszus, Anke; Gruen, Michael; Watzl, Bernhard; Ness, Christina; Roth, Alexander; Lochner, Alfred; Barz, Dagmar; Gabriel, Holger; Rothe, Michael; Jahreis, Gerhard

    2013-01-01

    Despite their beneficial anti-inflammatory properties, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) may increase the infection risk at high doses, likely by generating an immune-depressed state. To assess the contribution of different immune cell populations to the immunomodulatory fatty acid effect, we comparatively investigated several aspects of inflammation in human T-helper (Th) cells and monocytes. Both fatty acids, but DHA to a lesser extent compared with EPA, selectively...

  7. Effects of an open-label pilot study with high-dose EPA/DHA concentrates on plasma phospholipids and behavior in children with attention deficit hyperactivity disorder

    OpenAIRE

    Hutchins Heather L; Hallowell Edward M; Sorgi Paul J; Sears Barry

    2007-01-01

    Abstract Background Attention deficit hyperactivity disorder (ADHD) is the most common neurological condition in children. This pilot study evaluated the effects of high-dose eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) supplementation on the isolated plasma phospholipids and behavior in children with ADHD (primarily inattentive subtype and combined subtype). Methods Nine children were initially supplemented with 16.2 g EPA/DHA concentrates per day. The dosage was adjusted depen...

  8. DHA and EPA Down-regulate COX-2 Expression through Suppression of NF-κB Activity in LPS-treated Human Umbilical Vein Endothelial Cells

    OpenAIRE

    Lee, Soon Ae; Kim, Hye Jung; Chang, Ki Churl; Baek, Jong Chul; Park, Ji Kwon; Shin, Jeong Kyu; Choi, Won Jun; Lee, Jong Hak; Paik, Won Young

    2009-01-01

    Inflammatory processes of vascular endothelial cells play a key role in the development ofatherosclerosis. We determined the anti-inflammatory effects and mechanisms of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on LPS-treated human umbilical vein endothelial cells (HUVECs) to evaluate their cardioprotective potential. Cells were pretreated with DHA, EPA, or troglitazone prior to activation with LPS. Expression of COX-2, prostaglandin E2 (PGE2) and IL-6 production, and NF-κB a...

  9. EPA/DHA and vitamin A supplementation improves spatial memory and alleviates the age-related decrease in hippocampal RXRγ and kinase expression in rats

    OpenAIRE

    Anne eLétondor; Benjamin eBuaud; Carole eVaysse; Emmanuel eRichard; Sophie eLaye; Véronique ePallet; Serge eAlfos

    2016-01-01

    Studies suggest that eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and vitamin A are critical to delay aged-related cognitive decline. These nutrients regulate gene expression in the brain by binding to nuclear receptors such as the retinoid X receptors (RXRs) and the retinoic acid receptors (RARs). Moreover, EPA/DHA and retinoids activate notably kinase signaling pathways such as AKT or MAPK, which includes ERK1/2. This suggests that these nutrients may modulate brain function in a...

  10. DHA-Containing Oilseed: A Timely Solution for the Sustainability Issues Surrounding Fish Oil Sources of the Health-Benefitting Long-Chain Omega-3 Oils

    Directory of Open Access Journals (Sweden)

    Soressa M. Kitessa

    2014-05-01

    Full Text Available Benefits of long-chain (≥C20 omega-3 oils (LC omega-3 oils for reduction of the risk of a range of disorders are well documented. The benefits result from eicosapentaenoic acid (EPA and docosahexaenoic acid (DHA; optimal intake levels of these bioactive fatty acids for maintenance of normal health and prevention of diseases have been developed and adopted by national and international health agencies and science bodies. These developments have led to increased consumer demand for LC omega-3 oils and, coupled with increasing global population, will impact on future sustainable supply of fish. Seafood supply from aquaculture has risen over the past decades and it relies on harvest of wild catch fisheries also for its fish oil needs. Alternate sources of LC omega-3 oils are being pursued, including genetically modified soybean rich in shorter-chain stearidonic acid (SDA, 18:4ω3. However, neither oils from traditional oilseeds such as linseed, nor the SDA soybean oil have shown efficient conversion to DHA. A recent breakthrough has seen the demonstration of a land plant-based oil enriched in DHA, and with omega-6 PUFA levels close to that occurring in marine sources of EPA and DHA. We review alternative sources of DHA supply with emphasis on the need for land plant oils containing EPA and DHA.

  11. Wax Ester Rich Oil From The Marine Crustacean, Calanus finmarchicus, is a Bioavailable Source of EPA and DHA for Human Consumption.

    Science.gov (United States)

    Cook, Chad M; Larsen, Terje S; Derrig, Linda D; Kelly, Kathleen M; Tande, Kurt S

    2016-10-01

    Oil from the marine copepod, Calanus finmarchicus, which contains >86 % of fatty acids present as wax esters, is a novel source of n-3 fatty acids for human consumption. In a randomized, two-period crossover study, 18 healthy adults consumed 8 capsules providing 4 g of Calanus(®) Oil supplying a total of 260 mg EPA and 156 mg DHA primarily as wax esters, or 1 capsule of Lovaza(®) providing 465 mg EPA and 375 mg DHA as ethyl esters, each with an EPA- and DHA-free breakfast. Plasma EPA and DHA were measured over a 72 h period (t = 1, 2, 4, 6, 8, 10, 12, 24, 48, and 72 h). The positive incremental area under the curve over the 72 h test period (iAUC0-72 h) for both EPA and DHA was significantly different from zero (p wax ester rich marine oil is a suitable alternative source of EPA and DHA for human consumption.

  12. DHA-containing oilseed: a timely solution for the sustainability issues surrounding fish oil sources of the health-benefitting long-chain omega-3 oils.

    Science.gov (United States)

    Kitessa, Soressa M; Abeywardena, Mahinda; Wijesundera, Chakra; Nichols, Peter D

    2014-05-01

    Benefits of long-chain (≥C20) omega-3 oils (LC omega-3 oils) for reduction of the risk of a range of disorders are well documented. The benefits result from eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA); optimal intake levels of these bioactive fatty acids for maintenance of normal health and prevention of diseases have been developed and adopted by national and international health agencies and science bodies. These developments have led to increased consumer demand for LC omega-3 oils and, coupled with increasing global population, will impact on future sustainable supply of fish. Seafood supply from aquaculture has risen over the past decades and it relies on harvest of wild catch fisheries also for its fish oil needs. Alternate sources of LC omega-3 oils are being pursued, including genetically modified soybean rich in shorter-chain stearidonic acid (SDA, 18:4ω3). However, neither oils from traditional oilseeds such as linseed, nor the SDA soybean oil have shown efficient conversion to DHA. A recent breakthrough has seen the demonstration of a land plant-based oil enriched in DHA, and with omega-6 PUFA levels close to that occurring in marine sources of EPA and DHA. We review alternative sources of DHA supply with emphasis on the need for land plant oils containing EPA and DHA. PMID:24858407

  13. 氧化葡萄糖酸杆菌 DHA3-9的葡萄糖代谢酶系%Enzymatic system of glucose metabolism in Gluconobacter oxydans strain DHA3-9

    Institute of Scientific and Technical Information of China (English)

    李倩延; 卢向锋; 张鹏程; 孙玲艳; 刘于; 马晓航

    2014-01-01

    Summary Gluconobacter oxydans is widely used in industrial application for its dehydrogenase system locating on cell membrane.These dehydrogenases have a character to oxidize sugars and sugar alcohols incompletely. There are two potential pathways known for glucose oxidization in G.oxydans :More than 90% of glucose is transformed into gluconate in the periplasmic space;only a minority of glucose(about 5%)is phosphorylated and taken into functional central metabolic pathways such as Entner-Doudoroff pathway(EDP)and pentose phosphate pathway (PPP) in the cytoplasmic compartment.In previous study,the Embden-Meyerhof-Parnas pathway (EMP) was found inactive in G.oxydans due to its lack of phosphofructokinase. In this study,a G.oxydans strain named DHA3-9 was screened which produced dihydroxyacetone (DHA) during glucose degradation.But DHA was not a product in EDP or PPP.A mutant strain of G.oxydans DHA3-9 lacking of glucose dehydrogenase in cell membrane was constructed to study the possibility of other pathway of glucose metabolism in G.oxydans . A mgdh gene-disrupted mutant of G. oxydans DHA3-9 was constructed by the way of homologous recombination and its characteristic changes of the cells growth on glucose,glucose degradation,gluconate transformation,intermediate products and growth inhibition on acetate were studied. The results indicated that the growth of mutant strain on glucose showed an obvious delay and pH dropped much slower than that of wild type.The mutant lost most of its ability of glucose degradation and produced little gluconate.Instead,DHA formation of the mutant was recorded four times as that of wild type.Pyruvate and acetate were detected in the products of mutant whereas none of such products were found in wild type culture. Under the condition with glucose as the sole carbon source,50 mmol/L acetate completely inhibited the growth of mutant,whereas this effect was remarkably low on wild type. These results prove that in G. oxydans DHA3-9 mutant

  14. Mfsd2a Is a Transporter for the Essential ω-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development.

    Science.gov (United States)

    Wong, Bernice H; Chan, Jia Pei; Cazenave-Gassiot, Amaury; Poh, Rebecca W; Foo, Juat Chin; Galam, Dwight L A; Ghosh, Sujoy; Nguyen, Long N; Barathi, Veluchamy A; Yeo, Sia W; Luu, Chi D; Wenk, Markus R; Silver, David L

    2016-05-13

    Eye photoreceptor membrane discs in outer rod segments are highly enriched in the visual pigment rhodopsin and the ω-3 fatty acid docosahexaenoic acid (DHA). The eye acquires DHA from blood, but transporters for DHA uptake across the blood-retinal barrier or retinal pigment epithelium have not been identified. Mfsd2a is a newly described sodium-dependent lysophosphatidylcholine (LPC) symporter expressed at the blood-brain barrier that transports LPCs containing DHA and other long-chain fatty acids. LPC transport via Mfsd2a has been shown to be necessary for human brain growth. Here we demonstrate that Mfsd2a is highly expressed in retinal pigment epithelium in embryonic eye, before the development of photoreceptors, and is the primary site of Mfsd2a expression in the eye. Eyes from whole body Mfsd2a-deficient (KO) mice, but not endothelium-specific Mfsd2a-deficient mice, were DHA-deficient and had significantly reduced LPC/DHA transport in vivo Fluorescein angiography indicated normal blood-retinal barrier function. Histological and electron microscopic analysis indicated that Mfsd2a KO mice exhibited a specific reduction in outer rod segment length, disorganized outer rod segment discs, and mislocalization of and reduction in rhodopsin early in postnatal development without loss of photoreceptors. Minor photoreceptor cell loss occurred in adult Mfsd2a KO mice, but electroretinography indicated visual function was normal. The developing eyes of Mfsd2a KO mice had activated microglia and up-regulation of lipogenic and cholesterogenic genes, likely adaptations to loss of LPC transport. These findings identify LPC transport via Mfsd2a as an important pathway for DHA uptake in eye and for development of photoreceptor membrane discs. PMID:27008858

  15. Whole-body DHA synthesis-secretion kinetics from plasma eicosapentaenoic acid and alpha-linolenic acid in the free-living rat.

    Science.gov (United States)

    Metherel, Adam H; Domenichiello, Anthony F; Kitson, Alex P; Hopperton, Kathryn E; Bazinet, Richard P

    2016-09-01

    Whole body docosahexaenoic acid (DHA, 22:6n-3) synthesis from α-linolenic acid (ALA, 18:3n-3) is considered to be very low, however, the daily synthesis-secretion of DHA may be sufficient to supply the adult brain. The current study aims to assess whether whole body DHA synthesis-secretion kinetics are different when comparing plasma ALA versus eicosapentaenoic acid (EPA, 20:5n-3) as the precursor. Male Long Evans rats (n=6) were fed a 2% ALA in total fat diet for eight weeks, followed by surgery to implant a catheter into each of the jugular vein and carotid artery and 3h of steady-state infusion with a known amount of (2)H-ALA and (13)C-eicosapentaenoic acid (EPA, 20:5n3). Blood samples were collected at thirty-minute intervals and plasma enrichment of (2)H- and (13)C EPA, n-3 docosapentaenoic acid (DPAn-3, 22:5n-3) and DHA were determined for assessment of synthesis-secretion kinetic parameters. Results indicate a 13-fold higher synthesis-secretion coefficient for DHA from EPA as compared to ALA. However, after correcting for the 6.6 fold higher endogenous plasma ALA concentration, no significant differences in daily synthesis-secretion (nmol/day) of DHA (97.6±28.2 and 172±62), DPAn-3 (853±279 and 1139±484) or EPA (1587±592 and 1628±366) were observed from plasma unesterified ALA and EPA sources, respectively. These results suggest that typical diets which are significantly higher in ALA compared to EPA yield similar daily DHA synthesis-secretion despite a significantly higher synthesis-secretion coefficient from EPA. PMID:27263420

  16. The effects of Docosahexaenoic Acid (DHA on Helicobacter pylori eradication, some serum inflammatory factors and total antioxidant capacity

    Directory of Open Access Journals (Sweden)

    Shahram Agah

    2013-09-01

    Full Text Available Background: As regard to high prevalence of Helicobacter pylori infection and complications of it's persistence, as well as anti-bacterial activity against of Helicobacter pylori and anti-inflammatory properties of omega-3 fatty acids, this study was conducted to evaluate the effects of supplementation with Docosahexaenoic Acid (DHA on the eradication of Helicobacter pylori infection, some serum inflammatory markers and total antioxidant capacity.  Methods: In a double-blind, placebo-controlled clinical trial, 66 H. pylori positive patients (33 in the intervention group and 33 in the control group, along with tetra-drugs H. pylori eradication regimen, randomly received daily two grams morDHA supplement or Medium Chain Triglyceride (MCT oil as placebo for 12 weeks. Dietary intake data was collected by 24 hour food recall and analyzed by Nutritionist IV software. Sampling from fasting blood and measuring weight, height, body mass index (BMI and level of physical activity were done at the first and the end of the study. As well as, eradication test of the infection was performed for all patients at the end of the intervention.  Results: Eradication rate of the infection, the level of interleukin-6 (IL-6, high sensitivity C-reactive protein (hs-CRP and total antioxidant capacity (TAC didn't have significant difference between two groups at the end of the study (P>0.05, while the level of interleukin-8 (IL-8 was different between two groups (P=0.008. Difference of the concentration between the beginning and the end of the study was not significant in any factors between two groups (P>0.05.   Conclusion: Intake of morDHA supplement didn't have significant effect on the eradication of H. pylori, serum levels of IL-6, hs-CRP and TAC, while it was effective on the level of IL-8.

  17. 浅谈EPA和DHA的生理作用%Simply discuss the physiological function of EPA and DHA

    Institute of Scientific and Technical Information of China (English)

    赵春艳

    2010-01-01

    二十二碳六烯酸(DHA)和二十碳五烯酸(EPA)是人体必需的高度不饱和脂肪酸,它们对人类健康有特殊的作用和影响.DHA和EPA与生命现象密切相关,它们不但在视网膜和大脑的结构膜起重要作用,而且还是二十碳四烯酸(20∶4ω-6)代谢生成花生四烯酸(AA)的调节者.

  18. Estimation of microalgae genus through the fatty acid composition of the commercial microalgae DHA oil%通过商品化DHA藻油的脂肪酸组成推测其微藻属名

    Institute of Scientific and Technical Information of China (English)

    常桂芳; 王兴国

    2011-01-01

    DHA is an important functional omega -3 polyunsaturated fatty acid. Chinese Ministry of Health has successively approved mieroalgae DHA oils produced by Crpthecodinium cohnii , Schizochytrium sp. , Ulkenia sp. as novel resource food. Nine commercial microalgae DHA oils were collected and their fatty acid compositions were analyzed. The analysis result and related literature data showed that estimating microalgae genus through the fatty acid composition of the commercial microalgae DHA oil was possible. The long chain polyunsaturated fatty acids (LCPUFA) from Crpthecodinium cohnii contained only DHA and hardly DPA, and the LCPUFA from Schizochytrium sp. and Ulkenia sp. were composed of DHA and DPA. The ratio of DHA to DPA kept constant with the specific DHA microalgae, and had no significant changes on various fermentation conditions. For the ratio of DHA to DPA ranging 2 -3, the microalgae DHA oil was much possible from Schizochytrium sp.; while the ratio of DHA to DPA ranging 4 -6, the microalgae DHA oil may be from Schizochytrium sp. or Ulkenia sp.%DHA是一种重要的功能性ω-3多不饱和脂肪酸.我国卫生部相继批准了寇氏隐甲藻、裂壶藻、吾肯氏壶藻DHA藻油为新资源食品.收集并分析了9个商品化DHA藻油的脂肪酸含量,结合文献数据,发现通过商品化DHA藻油的脂肪酸特征推测其微藻属名是可行的.其中寇氏隐甲藻DHA藻油中长链多不饱和脂肪酸只含DHA,几乎不合DPA.裂壶藻与吾肯氏藻DHA藻油中长链多不饱脂肪酸主要以DHA和DPA为主,DHA/DPA比例随藻种而恒定,几乎不随发酵条件而有明显变化;DHA/DPA比例在2~3的DHA藻油多半来自裂壶藻;DHA/DPA比例在4~6的DHA藻油既可能来自裂壶藻也可能来自吾肯氏壶藻.

  19. EPA/DHA and vitamin A supplementation improves spatial memory and alleviates the age-related decrease in hippocampal RXRγ and kinase expression in rats

    Directory of Open Access Journals (Sweden)

    Anne eLétondor

    2016-05-01

    Full Text Available Studies suggest that eicosapentaenoic acid (EPA, docosahexaenoic acid (DHA and vitamin A are critical to delay aged-related cognitive decline. These nutrients regulate gene expression in the brain by binding to nuclear receptors such as the retinoid X receptors (RXRs and the retinoic acid receptors (RARs. Moreover, EPA/DHA and retinoids activate notably kinase signaling pathways such as AKT or MAPK, which includes ERK1/2. This suggests that these nutrients may modulate brain function in a similar way. Therefore we investigated in middle-aged rats the behavioral and molecular effects of supplementations with EPA/DHA and vitamin A alone or combined. 18-month-old rats exhibited reference and working memory deficits in the Morris water maze, associated with a decrease in serum vitamin A and hippocampal EPA/DHA contents. RARα, RXRβ and RXRγ mRNA expression and CAMKII, AKT, ERK1/2 expression were decreased in the hippocampus of middle-aged rats. A combined EPA/DHA and vitamin A supplementation had a beneficial additive effect on reference memory but not in working memory in middle-aged rats, associated with an alleviation of the age-related decrease in RXRγ, CAMKII, AKT and ERK1 expression in the hippocampus. This study provides a new combined nutritional strategy to delay brain aging.

  20. EPA/DHA and Vitamin A Supplementation Improves Spatial Memory and Alleviates the Age-related Decrease in Hippocampal RXRγ and Kinase Expression in Rats.

    Science.gov (United States)

    Létondor, Anne; Buaud, Benjamin; Vaysse, Carole; Richard, Emmanuel; Layé, Sophie; Pallet, Véronique; Alfos, Serge

    2016-01-01

    Studies suggest that eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and vitamin A are critical to delay aged-related cognitive decline. These nutrients regulate gene expression in the brain by binding to nuclear receptors such as the retinoid X receptors (RXRs) and the retinoic acid receptors (RARs). Moreover, EPA/DHA and retinoids activate notably kinase signaling pathways such as AKT or MAPK, which includes ERK1/2. This suggests that these nutrients may modulate brain function in a similar way. Therefore, we investigated in middle-aged rats the behavioral and molecular effects of supplementations with EPA/DHA and vitamin A alone or combined. 18-month-old rats exhibited reference and working memory deficits in the Morris water maze, associated with a decrease in serum vitamin A and hippocampal EPA/DHA contents. RARα, RXRβ, and RXRγ mRNA expression and CAMKII, AKT, ERK1/2 expression were decreased in the hippocampus of middle-aged rats. A combined EPA/DHA and vitamin A supplementation had a beneficial additive effect on reference memory but not in working memory in middle-aged rats, associated with an alleviation of the age-related decrease in RXRγ, CAMKII, AKT, and ERK1 expression in the hippocampus. This study provides a new combined nutritional strategy to delay brain aging. PMID:27242514

  1. Determination of the load state of lead-acid batteries using neural networks; Determinacion del estado de carga de baterias plomo-acido utilizando redes neuronales

    Energy Technology Data Exchange (ETDEWEB)

    Cristin V, Miguel A; Ortega S, Cesar A [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2005-07-01

    The charge of lead-acid batteries (LAB), as in any other type of batteries, consists of replacing the energy consumed during the discharge. Nevertheless, as no physical or chemical process is good enough to totality recharge a battery, it is necessary to supply to it more than the 100% of the energy demanded during its discharge. A critical factor to make a suitable load control of the batteries is to determine its own state of load. That is to say, to have an efficient load control, it is necessary to count on means that allow to accurately determining the residual capacity of the battery to deliver load. This one is the one of the aspects of greater interest in the research centers around world. For this reason, in this work it was pretended to develop a calculation algorithm of the state of load of batteries based on a fuzzy-neural network that could calculate the state of load without using the battery current as an input. This is because one of the main problems for the designers of battery load controllers is the correct supervision of the current that circulates around the system in all the rank of operation of the same one because the sensors do not have a linear behavior. [Spanish] La recarga de baterias plomo-acido (BPA), como cualquier otro tipo de baterias, consiste en reponer la energia consumida durante la descarga. Sin embargo, como ningun proceso fisico o quimico es lo bastante eficiente para recargar a totalidad una bateria, es necesario suministrarle mas del 100% de la energia demandada durante su descarga. Un factor critico para realizar un adecuado control de carga de las baterias, es determinar su propio estado de carga. Es decir, para tener un control de carga eficiente, es necesario contar con un medio que permita determinar con precision la capacidad remanente de la bateria para entregar carga. Este es uno de los aspectos de mayor interes en los centros de investigacion alrededor el mundo. Por tal razon, en este trabajo se propuso

  2. 饲料中DHA/EPA值对星斑川鲽幼鱼生长、体组成及血清生理指标的影响%Effect of dietary DHA to EPA ratios on growth performance,body composition and serum physiological parameters in juvenile Platichthys stellatus

    Institute of Scientific and Technical Information of China (English)

    马晶晶; 王际英; 孙建珍; 郝甜甜; 张德瑞; 张利民

    2014-01-01

    为研究饲料DHA/EPA值对星斑川鲽幼鱼生长、体组成和血液生理指标的影响,实验配制等氮、等能的5种不同DHA/EPA值(0.64、0.97、1.18、1.59和1.91)的饲料,每个比值设3个重复,饲养周期56 d.结果显示:(1)随着饲料DHA/EPA值的升高,星斑川鲽幼鱼增重率(WGR)、饲料效率(FE)、蛋白质效率(PER)均呈先上升后下降的趋势(P<0.05).当饲料DHA/EPA值为0.97 ~ 1.59时实验鱼增重最快,饲料效率最高.蛋白质效率则在DHA/EPA值为0.97 ~1.18时达到最高.蛋白质沉积率(protein retention efficiency,PRE)与饲料DHA/EPA值呈显著二次回归关系(y=-1.589 5x2 +2.858 3x +45.184;R2 =0.910 8,x=饲料DHA/EPA值,y=PRE),当饲料DHA/EPA值大于0.90时呈下降趋势.肝体比(HSI)呈先下降后小幅回升的趋势(P<0.05),在饲料DHA/EPA值为1.18时达到最低,为2.85%,脾脏指数(SSI)呈显著上升趋势(P<0.05),于饲料DHA/EPA值为1.59组最高(0.12%);(2)肝脏粗脂肪含量随饲料DHA/EPA值的增加呈明显下降趋势(P<0.05),且在饲料DHA/EPA值为1.18时降到最低,为8.60%,而后又显著上升,但仍显著低于饲料DHA/EPA值为0.64时的水平(13.44%).二次回归分析(y=5.1996x2-15.652x+20.866;R2 =0.634 8,x=饲料DHA/EPA值,y=肝脏脂肪含量)显示,当饲料中DHA/EPA值为1.51时肝脏脂肪含量最低.脂肪酸分析结果显示,随着饲料DHA/EPA值的升高,肝脏及肌肉中EPA含量均呈线性下降趋势(P<0.05),而DHA含量及DHA/EPA均呈直线上升趋势(P<0.05).肝脏和肌肉组织n-3 HUFA总量不受饲料处理的影响(P>0.05);(3)血清总蛋白、球蛋白含量在饲料DHA/EPA值为1.59时显著高于其他各组(P<0.05),白蛋白在饲料DHA/EPA值为0.64、0.97和1.59水平最高.溶菌酶(LSZ)活性在饲料DHA/EPA值为1.18时达到峰值(P<0.05),为2.76 μg/mL.谷丙转氨酶(ALT)活性在饲料DHA/EPA值<1.91时无显著变化,而当饲料DHA/EPA值>1.18时,血清谷草转氨酶(AST)活性提高了65

  3. Inadequate supply of vitamins and DHA in the elderly: implications for brain aging and Alzheimer-type dementia.

    Science.gov (United States)

    Mohajeri, M Hasan; Troesch, Barbara; Weber, Peter

    2015-02-01

    Alzheimer's disease (AD) is the most prevalent, severe, and disabling cause of dementia worldwide. To date, AD therapy is primarily targeted toward palliative treatment of symptoms rather than prevention of disease progression. So far, no pharmacologic interventions have changed the onset or progression of AD and their use is accompanied by side effects. The major obstacle in managing AD and designing therapeutic strategies is the difficulty in retarding neuronal loss in the diseased brain once the pathologic events leading to neuronal death have started. Therefore, a promising alternative strategy is to maintain a healthy neuronal population in the aging brain for as long as possible. One factor evidently important for neuronal health and function is the optimal supply of nutrients necessary for maintaining normal functioning of the brain. Mechanistic studies, epidemiologic analyses, and randomized controlled intervention trials provide insight to the positive effects of docosahexaenoic acid (DHA) and micronutrients such as the vitamin B family, and vitamins E, C, and D, in helping neurons to cope with aging. These nutrients are inexpensive in use, have virtually no side effects when used at recommended doses, are essential for life, have established modes of action, and are broadly accepted by the general public. This review provides some evidence that the use of vitamins and DHA for the aging population in general, and for individuals at risk in particular, is a viable alternative approach to delaying brain aging and for protecting against the onset of AD pathology. PMID:25592004

  4. Healthy effect of different proportions of marine ω-3 PUFAs EPA and DHA supplementation in Wistar rats: Lipidomic biomarkers of oxidative stress and inflammation.

    Science.gov (United States)

    Dasilva, Gabriel; Pazos, Manuel; García-Egido, Eduardo; Gallardo, Jose Manuel; Rodríguez, Isaac; Cela, Rafael; Medina, Isabel

    2015-11-01

    Dietary intervention with ω-3 marine fatty acids may potentially modulate inflammation and oxidative stress markers related with CVD, metabolic syndrome and cancer. The aim of this study was to evaluate whether different proportions of ω-3 EPA and DHA intake provoke a modulation of the production of lipid mediators and then, an influence on different indexes of inflammation and oxidative stress in a controlled dietary animal experiment using Wistar rats. For such scope, a lipidomic SPE-LC-ESI-MS/MS approach previously developed was applied to determine lipid mediators profile in plasma samples. The effect of ω-3 fatty acids associated to different ratios EPA:DHA was compared with the effect exerted by ω-3 ALA supplementation from linseed oil and ω-6 LA from soybean oil. CRP showed a tendency to greater inflammatory status in all ω-3-fed animals. Interestingly, ratios 1:1 and 2:1 EPA:DHA evidenced a noteworthy healthy effect generating a less oxidative environment and modulating LOX and COX activities toward a decrease in the production of proinflammatory ARA eicosanoids and oxidative stress biomarkers from EPA and DHA. In addition, the ability of 1:1 and 2:1 fish oil diets to reduce lipid mediator levels was in concurrence with the protective effect exerted by decreasing inflammatory markers as ω-6/ω-3 ratio in plasma and membranes. It was also highlighted the effect of a higher DHA amount in the diet reducing the healthy benefits described in terms of inflammation and oxidative stress. Results support the antiinflammatory and antioxidative role of fish oils and, particularly, the effect of adequate proportions EPA:DHA. PMID:26320676

  5. Extraction and Purification of EPA and DHA from Microalgae%从微藻中提取分离EPA和DHA的方法

    Institute of Scientific and Technical Information of China (English)

    周冉; 王飞; 常明; 岳红坤; 史兰香; 刘司婕

    2012-01-01

    With important medical and healthcare functions, EPA (eicosapentaenoic acid) and DHA (docosahexaenoicacid) are becoming more and more popular. Compared with the deep-sea fish, the microalgae are easy to get and contain higher content of EPA and DHA. In this paper, several common and potential methods to extract EPA and DHA from the microalgae were introduced, including solvent extraction, supercritical fluid extraction, urea inclusion, high performance liquid chromatography, supercritical fluid chromatography and lipase-catalyzed reactions. Both the advantages and disadvantages of these methods were discussed, and their application prospects were forecasted, aiming to provide basis for the extraction and isolation of EPA and DHA from the microaigae.%EPA( eicosapentaenoic acid,EPA)和DHA (docosahexaenoic acid,DHA)由于具有很重要的医疗和保健功效,越来越受到研究者的青睐.与深海鱼相比,微藻材料来源较易,其EPA和DHA的含量更高,是更好的生产EPA和DHA的原料.该研究介绍了几种常用和具有开发潜力的从微藻中提取分离EPA和DHA的方法,包括溶剂提取法、超临界流体萃取法、尿素包合法、高效液相色谱法、超临界流体色谱法和脂肪酶催化反应法,并通过探讨各种方法的优缺点,对其应用前景进行了展望,以期为从微藻中提取分离EPA和DHA的研究提供参考依据.

  6. EFECTO DEL ENRIQUECIMIENTO DE Artemia FRANCISCANA CON DIFERENTES PROPORCIONES DE ω-3 DHA/EPA SOBRE EL CRECIMIENTO DE JUVENILES DE PEZ BLANCO Menidia estor.

    OpenAIRE

    Hernández Téllez, Ana Rosa

    2012-01-01

    La inclusión de los ácidos grasos ω-3 DHA y EPA en el alimento, es un factor importante para lograr mejor supervivencia y crecimiento en la mayoría de los peces cultivados. Dentro del desarrollo tecnológico para el cultivo del pez blanco de Pátzcuaro Menidia estor, se desconoce el requerimiento de ácidos grasos ω-3 DHA/EPA, y el efecto que éstos tienen en el crecimiento y supervivencia de juveniles en cautiverio. En esta especie como en otras, el alimento vivo (nauplio de Artem...

  7. Characterization of Oilseed Lipids from “DHA-Producing Camelina sativa”: A New Transformed Land Plant Containing Long-Chain Omega-3 Oils

    OpenAIRE

    Maged P. Mansour; Pushkar Shrestha; Srinivas Belide; Petrie, James R.; Peter D Nichols; Surinder P. Singh

    2014-01-01

    New and sustainable sources of long-chain (LC, ≥C20) omega-3 oils containing DHA (docosahexaenoic acid, 22:6ω3) are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG) as the major lipid class in hexane extracts (96% of total lipid). Subsequent chloroform-methanol (CM) extraction recovered fur...

  8. 海藻EPA、DHA含量及分离浓缩方法%EPA and DHA Content of Marine Algae and Their Separation Concentration Methods

    Institute of Scientific and Technical Information of China (English)

    胡爱军; 丘泰球; 梁汉华

    2002-01-01

    EPA和DHA具有多种重要的生理功能,许多海藻富含EPA或DHA,利用海藻作为生产EPA或DHA的又一来源具有巨大的潜力和十分重要的意义.本文论述了EPA和DHA的营养学功能、天然海藻和人工培养的海藻EPA、DHA含量,并阐述了EPA、DHA的分离浓缩方法.

  9. The influence of EPA and DHA on markers of inflammation in 3T3-L1 cells at different stages of cellular maturation

    OpenAIRE

    Prostek, Adam; Gajewska, Małgorzata; Kamola, Dariusz; Bałasińska, Bożena

    2014-01-01

    Background EPA and DHA have been reported to have anti-obesity and anti-inflammatory properties. Recent studies revealed that these positive actions of n-3 PUFA at least partially are connected with their influence on metabolism and secretory functions of the adipose tissue. However, their impact on old adipocytes is still poorly understood. Therefore the aim of the present study was to evaluate the influence of EPA and DHA on markers of inflammation in 3T3-L1 cells at different stages of cel...

  10. DHA Inhibits Protein Degradation More Efficiently than EPA by Regulating the PPARγ/NFκB Pathway in C2C12 Myotubes

    OpenAIRE

    Yue Wang; Qiao-wei Lin; Pei-pei Zheng; Jian-song Zhang; Fei-ruo Huang

    2013-01-01

    This study was conducted to evaluate the mechanism by which n-3 PUFA regulated the protein degradation in C2C12 myotubes. Compared with the BSA control, EPA at concentrations from 400 to 600 µM decreased total protein degradation (P < 0.01). However, the total protein degradation was decreased when the concentrations of DHA ranged from 300 µM to 700 µM (P < 0.01). DHA (400 µM, 24 h) more efficiently decreased the I κ B α phosphorylation and increased in the I κ B α protein level than 400 µM E...

  11. Proposal to neutralize acid fluids from wells in the Los Humeros, Pue., geothermal field; Propuesta para la neutralizacion de fluidos acidos provenientes de pozos del campo geotermico de Los Humeros, Pue.

    Energy Technology Data Exchange (ETDEWEB)

    Flores Armenta, Magaly del Carmen; Ramirez Montes, Miguel; Garcia Cuevas, Juan Manuel [Comision Federal de Electricidad, Gerencia de Proyectos Geotermoelectricos, Morelia, Michoacan (Mexico)]. E-mail: magaly.flores@cfe.gob.mx

    2009-07-15

    Neutralizing an acidic fluid consists of adding a sodium hydroxide solution to neutralize the H group of acids, therefore increasing the pH. The injection of sodium hydroxide has to be continuous and at a proper depth inside the well to protect against the corrosion of casing and surface equipment. Neutralization is a common practice used in geothermal fields, such as at The Geysers in the US and Miravalles in Costa Rica-places where aggressive fluids cause problems for extracting and using geothermal fluids commercially. A zone surrounding wells H-4, H-16 and H-29 in the northern section of the Los Humeros, Pue., geothermal field, known as the Colapso Central, has shown evidence of aggressively acidic fluids. Several wells drilled in the area had to be repaired, thus plugging and isolating the deepest production zones. Well H-43 was drilled two years ago in the northern zone of the field, and even though it is not located in the aggressive-fluid zone, the well presents mineralogical features possibly indicating the presence of acidic fluids. Therefore, before producing this well it has been proposed we install a neutralization system with general characteristics presented in this paper. The system will prevent corrosion that up to now has prevented exploitation of the deep portion of Colapso Central, helping to develop the field in a more profitable way. [Spanish] Neutralizar un fluido acido consiste en agregarle una solucion de hidroxido de sodio. Esto neutraliza el grupo de acidos H y en consecuencia aumenta el pH. La inyeccion de hidroxido de sodio se realiza de manera continua y a una profundidad adecuada dentro del pozo para proteger a la tuberia y a todo el equipo superficial contra la corrosion. La neutralizacion es una practica comun que se viene realizando en campos como Los Geysers en Estados Unidos y en Miravalles, Costa Rica, donde la presencia de fluidos agresivos causa problemas en la extraccion y aprovechamiento del fluido geotermico con fines

  12. Impact of different ratios of Omega-6 polyundaturated fatty acids to eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA) on atherosclerotic lesion formation and inflammatory factors in the LDL receptor knockout mouse

    Science.gov (United States)

    The objective was to assess the effect of different ratios of omega-6 to EPA plus DHA on atherosclerotic lesion formation and plasma inflammatory markers in LDLr-/- mice. Mice (n=10/group) were fed the following diets for 32 weeks: high fat (20% w/w) without EPA and DHA (HF omega-6), and high fat wi...

  13. 金藻Isochrysis Galbana中DHA的分离与分析%Separation and Analysis DHA from Isochrysis Galbana

    Institute of Scientific and Technical Information of China (English)

    李润; 张万宽; 倪杭生; 戴俊彪; 王宏; 周春玉

    2001-01-01

    以金藻作为二十二碳六烯酸(DHA)的新来源,对金藻中的脂肪酸分别用氯仿、甲醇、水(体积比为1:2:0.8)的混合液进行萃取分离和BF3、无水乙醇(体积比1:3)作酯化剂乙酯化.用硅胶薄板检验乙酯化程度.利用气相色谱(GC)和气相色谱-质谱联用仪(GC/MS)进行定性分析,用内标法对DHA进行定量分析,C19脂肪酸乙酯作内标.测得藻粉中DHA含量为2.2%.

  14. Lipidomics to analyze the influence of diets with different EPA:DHA ratios in the progression of Metabolic Syndrome using SHROB rats as a model.

    Science.gov (United States)

    Dasilva, Gabriel; Pazos, Manuel; García-Egido, Eduardo; Pérez-Jiménez, Jara; Torres, Josep Lluis; Giralt, Montserrat; Nogués, María-Rosa; Medina, Isabel

    2016-08-15

    The role of specific proportions of ω-3 EPA and DHA, in the modulation of inflammation and oxidative stress markers associated to the progression of Metabolic Syndrome was investigated. Potential inflammatory eicosanoids and docosanoids were discussed together to biomarkers of CVD, obesity, inflammation and oxidative stress in an animal model of metabolic disorders. Results evidenced a noteworthy health effect of 1:1 and 2:1 EPA:DHA proportions over 1:2 EPA:DHA based diets through a down-regulation in the production of strong pro-inflammatory ω-6 eicosanoids, a decrement of biomarkers of oxidative stress, and a modulation of fatty acid desaturase activities and plasma and membrane PUFAs towards greater anti-inflammatory profiles. Outcomes contribute to the general knowledge on the health benefits of marine lipids and their role on the progress of MetS, inflammation and oxidative stress. Results shed light on controversial protective mechanisms of EPA and DHA to better design dietary interventions aimed at reducing MetS. PMID:27006231

  15. The omega-3 fatty acid DHA dose-dependently reduces atherosclerosis: a putative role for F4-neuroprostanes a specific class of peroxidized metabolites

    Science.gov (United States)

    Objective. Consumption of long chain omega-3 polyunsaturated fatty acids is associated with reduced risks of cardiovascular disease but the role of their oxygenated metabolites remains unclear. We hypothesized that peroxidized metabolites of docosahexaenoic acid (DHA, 22:6 n-3) could play a role in ...

  16. The ethanolamide metabolite of DHA, docosahexaenoylethanolamine, shows immunomodulating effects in mouse peritoneal and RAW264.7 macrophages: Evidence for a new link between fish oil and inflammation

    NARCIS (Netherlands)

    Meijerink, J.; Plastina, P.; Vincken, J.P.; Poland, M.; Attya, M.; Balvers, M.; Gruppen, H.; Gabriele, B.; Witkamp, R.F.

    2011-01-01

    Several mechanisms have been proposed for the positive health effects associated with dietary consumption of long-chain n-3 PUFA (n-3 LC-PUFA) including DHA (22 : 6n-3) and EPA (20 : 5n-3). After dietary intake, LC-PUFA are incorporated into membranes and can be converted to their corresponding N-ac

  17. Decreased arachidonic acid content and metabolism in tissues of NZB/W F1 females fed a diet containing 0. 45% dehydroisoandrosterone (DHA)

    Energy Technology Data Exchange (ETDEWEB)

    Matsunaga, A.; Cottam, G.L.

    1987-05-01

    A diet containing 0.45% DHA fed to NZB/W mice, a model of systemic lupus erythematosus, delays the time of onset, improves survival and decreases the formation of antibodies to ds-DNA. Essential fatty acid-deficient diets or inclusion of eicosapentaenoic acid have similar beneficial effects and led them to investigate arachidonic acid metabolism in response to feeding DHA. The arachidonic acid content of plasma cholesteryl ester decreased from 37.4 +/- 2.2 to 28.2 +/- 1.3 mg%. In total liver phospholipid the value decreased from 18.1 +/- 0.52 to 13.7 +/- 1.3 mg%, in total kidney phospholipid the value decreased from 24.10 +/- 0.87 to 20.7 +/- 0.32 mg% and in resident peritoneal macrophages the value decreased from 15.4 +/- 4.6 to 3.6 +/- 1.4 mg%. The metabolism of exogenous (1-/sup 14/C)arachidonic acid by resident peritoneal macrophages in response to Zymosan stimulation for 2 hr was examined by extraction of metabolites and separation by HPLC. Cells isolated from DHA-fed animals produced less PGE2 than controls, yet similar amounts of 6-keto PGF1..cap alpha.. were produced. Arachidonic acid metabolites have significant effects on the immune system and may be a mechanism involved in the benefits obtained by inclusion of DHA in the diet.

  18. 高含量DHA薇藻食用油的应用可行性研究%The application research of high levels of DHA in edible oil

    Institute of Scientific and Technical Information of China (English)

    司华静; 彭晓芳; 马金余

    2011-01-01

    研究了添加高含量DHA藻油的花生油和橄榄油(20%DHA净含量)的保质期;考察了其在烹调(160℃)条件下DHA的稳定性以及DHA藻油对食用油感官品质的影响.实验结果表明:高含量DHA的橄榄油和花生油(20%DHA净含量)在62℃条件下储存28d后,未出现哈败及藻味;与未加热的DHA食用油相比,100℃烹调20min后DHA含量基本无变化,而在160℃烹调20min,DHA含量会有稍许降低,但不明显.且DHA高含量的添加不会对橄榄油、花生油的原本口感和气味产生明显影响.%The shelf - life of peanut oil and olive oil containing 20% DHA was studied, respectively; The thermal stability of DHA under cooking conditions with low temperatures was examined, and the sensory evaluation of olive oil and peanut oil with 20% DHA was also conducted to observe potential changes caused by DHA; Results showed that: peanut oil and olive oil with 20% DHA can be stored for 28 days at 62℃ without rancid flavor.

  19. Emergence of DHA-1-producing Klebsiella spp. in the Parisian region: genetic organization of the ampC and ampR genes originating from Morganella morganii.

    Science.gov (United States)

    Verdet, Charlotte; Benzerara, Yahia; Gautier, Valérie; Adam, Olivier; Ould-Hocine, Zahia; Arlet, Guillaume

    2006-02-01

    Eleven Klebsiella pneumoniae clinical isolates and one Klebsiella oxytoca clinical isolate showing various pulsed-field gel electrophoresis types and producing an inducible DHA-1 class C beta-lactamase were isolated in the Parisian region between 1998 and 2003. The aim of this study was to compare the genetic organization of the bla(DHA-1) genes in this collection of clinical isolates. In four isolates, the Morganella morganii-derived genomic region containing bla(DHA-1) was inserted in an entire complex sul1-type integron, including a region common to In6-In7 (CR1), as previously described in a bla(DHA-1)-producing Salmonella enterica serovar Enteritidis KF92 isolate from Saudi Arabia in 1992. Different gene cassette arrays were characterized in each of these integrons. In two of them, an additional 10-kb fragment was inserted between the CR1 and the M. morganii-derived region and was similar to the sap (ABC transporter family) and psp (phage shock protein) operons originated from Salmonella enterica serovar Typhimurium. The length of the M. morganii region was variable, suggesting that several independent recombination events have occurred and that open reading frame orf513 encodes a recombinase involved in the mobilization of the resistance genes. The genetic organization of bla(DHA-1) was identical in the eight other isolates. This structure is likely derived from a complex integron following the insertion of IS26, leading to the deletion of the first part of integron. The horizontal transfer of one plasmid carrying that truncated integron was shown for seven of these isolates.

  20. Characterization of Oilseed Lipids from “DHA-Producing Camelina sativa”: A New Transformed Land Plant Containing Long-Chain Omega-3 Oils

    Directory of Open Access Journals (Sweden)

    Maged P. Mansour

    2014-02-01

    Full Text Available New and sustainable sources of long-chain (LC, ≥C20 omega-3 oils containing DHA (docosahexaenoic acid, 22:6ω3 are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG as the major lipid class in hexane extracts (96% of total lipid. Subsequent chloroform-methanol (CM extraction recovered further lipid (~50% polar lipid, comprising glycolipids and phospholipids and residual TAG. The main phospholipid species were phosphatidyl choline and phosphatidyl ethanolamine. The % DHA was: 6.8% (of total fatty acids in the TAG-rich hexane extract and 4.2% in the polar lipid-rich CM extract. The relative level of ALA (α-linolenic acid, 18:3ω3 in DHA-camelina seed was higher than the control. Major sterols in both DHA- and control camelina seeds were: sitosterol, campesterol, cholesterol, brassicasterol and isofucosterol. C16–C22 fatty alcohols, including iso-branched and odd-chain alcohols were present, including high levels of iso-17:0, 17:0 and 19:0. Other alcohols present were: 16:0, iso-18:0, 18:0 and 18:1 and the proportions varied between the hexane and CM extracts. These iso-branched odd-chain fatty alcohols, to our knowledge, have not been previously reported. These components may be derived from wax esters, or free fatty alcohols.

  1. Characterization of oilseed lipids from "DHA-producing Camelina sativa": a new transformed land plant containing long-chain omega-3 oils.

    Science.gov (United States)

    Mansour, Maged P; Shrestha, Pushkar; Belide, Srinivas; Petrie, James R; Nichols, Peter D; Singh, Surinder P

    2014-02-21

    New and sustainable sources of long-chain (LC, ≥C₂₀) omega-3 oils containing DHA (docosahexaenoic acid, 22:6ω3) are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG) as the major lipid class in hexane extracts (96% of total lipid). Subsequent chloroform-methanol (CM) extraction recovered further lipid (~50% polar lipid, comprising glycolipids and phospholipids) and residual TAG. The main phospholipid species were phosphatidyl choline and phosphatidyl ethanolamine. The % DHA was: 6.8% (of total fatty acids) in the TAG-rich hexane extract and 4.2% in the polar lipid-rich CM extract. The relative level of ALA (α-linolenic acid, 18:3ω3) in DHA-camelina seed was higher than the control. Major sterols in both DHA- and control camelina seeds were: sitosterol, campesterol, cholesterol, brassicasterol and isofucosterol. C₁₆-C₂₂ fatty alcohols, including iso-branched and odd-chain alcohols were present, including high levels of iso-17:0, 17:0 and 19:0. Other alcohols present were: 16:0, iso-18:0, 18:0 and 18:1 and the proportions varied between the hexane and CM extracts. These iso-branched odd-chain fatty alcohols, to our knowledge, have not been previously reported. These components may be derived from wax esters, or free fatty alcohols.

  2. Mildly abnormal general movement quality in infants is associated with higher Mead acid and lower arachidonic acid and shows a U-shaped relation with the DHA/AA ratio.

    Science.gov (United States)

    van Goor, S A; Schaafsma, A; Erwich, J J H M; Dijck-Brouwer, D A J; Muskiet, F A J

    2010-01-01

    We showed that docosahexaenoic acid (DHA) supplementation during pregnancy and lactation was associated with more mildly abnormal (MA) general movements (GMs) in the infants. Since this finding was unexpected and inter-individual DHA intakes are highly variable, we explored the relationship between GM quality and erythrocyte DHA, arachidonic acid (AA), DHA/AA and Mead acid in 57 infants of this trial. MA GMs were inversely related to AA, associated with Mead acid, and associated with DHA/AA in a U-shaped manner. These relationships may indicate dependence of newborn AA status on synthesis from linoleic acid. This becomes restricted during the intrauterine period by abundant de novo synthesis of oleic and Mead acids from glucose, consistent with reduced insulin sensitivity during the third trimester. The descending part of the U-shaped relation between MA GMs and DHA/AA probably indicates DHA shortage next to AA shortage. The ascending part may reflect a different developmental trajectory that is not necessarily unfavorable. PMID:20022733

  3. Low validity of self-report in identifying recent mental health diagnosis among U.S. service members completing Pre-Deployment Health Assessment (PreDHA) and deployed to Afghanistan, 2007: a retrospective cohort study

    OpenAIRE

    Nevin Remington L

    2009-01-01

    Abstract Background Since 1998, the U.S. Armed Forces has used the mandatory Pre-Deployment Health Assessment (PreDHA) screening questionnaire as a means of assessing the health and suitability of U.S. service members for deployment. Limited data exists to quantify the validity of the self-reported PreDHA. This study was conducted to assess the validity of self-reporting in PreDHA to identify deployed service members who have had a recent mental health disorder diagnosis. Methods A retrospect...

  4. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion related to the Tolerable Upper Intake Level of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA)

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to deliver a scientific opinion on the Tolerable Upper Intake Level (UL) of the n-3 LCPUFAs eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA...... 6 g/day, and of DHA at doses of 2 4 g/day, induce an increase in LDL-cholesterol concentrations of about 3 % which may not have an adverse effect on cardiovascular disease risk, whereas EPA at doses up to 4 g/day has no significant effect on LDL cholesterol. Supplemental intakes of EPA and DHA...

  5. The effect of DHA/EPA ratio in live feed on the fatty acid composition, survival, growth and pigmentation of turbot larvae Scophthalmus maximus L.

    OpenAIRE

    Curé, K.; Gajardo, G.; Coutteau, P.

    1996-01-01

    A set of experiments was designed to understand the effect of standardized enrichment diets on the fatty acid composition and culture performance of turbot larvae reared using the food chain rotifers -freshly hatched Artemia nauplii- enriched Artemia . Rotifers were fed on experimental diets of similar total n-3 HUFA content (34-39 mg/g DW) but different DHA/EPA ratio (0.7, 2.6 and 5.5). DHA/EPA ratio in Artemia was manipulated by 24-h enrichment with an emulsion with either a high or low DHA...

  6. Concentração de ácido eicosapentaenóico (EPA) e ácido docosahexaenóico (DHA) em peixes marinhos da costa brasileira

    OpenAIRE

    Visentainer, Jesuí Vergilio; Carvalho, Patrícia de Oliveira; Ikegaki, Masaharu; PARK Yong Kum

    2000-01-01

    Análises experimentais foram realizadas objetivando a composição quantitativa dos ácidos graxos Eicosapentaenóico (EPA) e Docosahexaenóico (DHA), em diferentes partes do corpo de espécies de peixes marinhos da costa brasileira (atum, bonito, olho de boi, cavalinha, sardinha e serra). Os teores de EPA e DHA foram analisados em duas partes distintas: olho (órbita ocular e material gorduroso da cavidade ocular) e filés, sendo significativas as diferenças entre as mesmas. Os teores de DHA para um...

  7. Separation of EPA and DHA by Ag+modified amino silica gel column%载银氨基硅胶柱分离EPA和DHA的研究

    Institute of Scientific and Technical Information of China (English)

    邢飞; 朱靖博; 丁燕; 寇自农

    2014-01-01

    利用负载银离子的氨基硅胶为固定相,制备4.6 mm ×250 mm载银氨基硅胶柱,系统评价EPA和DHA在正相体系中的色谱保留行为,考察了载银氨基硅胶的粒径和流动相对EPA和DHA分离的影响。结果表明:5μm粒径的载银氨基硅胶比10μm粒径的分离度好;随着流动相中正己烷比例的增大,EPA和DHA分离度增大;当选用5μm载银氨基硅胶、流动相为正己烷-异丁醇(体积比2000∶1)、流速1 mL/min、紫外检测波长210 nm、进样量5μL时,EPA和DHA的分离度最佳,分离度为2.03。该方法简单、快速、重现性好,可用于EPA和DHA的分离。%The analysis column (4. 6 mm × 250 mm) for the separation of EPA and DHA was prepared with Ag+ modified amino silica gel as stationary phase. A systematic evaluation on retention behavior of EPA and DHA in normal phase chromatography was performed. The influences of Ag+ modified amino silica gel with different pore sizes and mobile phase on the separation of EPA and DHA were researched. The results showed that the separation degree of 5 μm Ag+ modified amino silica gel was better than that of 10 μm Ag+ modified amino silica gel;the separation degree of EPA and DHA increased with the pro-portion of n-hexane in mobile phase increasing;when the pore size of Ag+ modified amino silica gel was 5 μm, the volume ratio of n-hexane to isobutanol was 2 000∶1, flow rate was 1 mL/min, ultraviolet de-tection wavelength was 210 nm and the injection volume was 5 μL, the separation degree of EPA and DHA was the best and reached 2. 03. The method had the items of simpleness, rapidness and high repro-ducibility, which was suitable for the separation of EPA and DHA.

  8. Synthesis and characterization of PdAg as a catalyst for oxygen reduction reaction in acid medium; Sintesis y caracterizacion de PdAg como catalizador para la reaccion de reduccion de oxigeno en medio acido

    Energy Technology Data Exchange (ETDEWEB)

    Martinez-Casillas, D. C.; Vazquez-Huerta, G.; Solorza-Feria, O. [Centro de Investigacion y de Estudios Avanzados del IPN, Mexico D.F. (Mexico)]. E-mail: dcmartinez@cinvestav.mx

    2009-09-15

    This work presents the synthesis of the binary compound PdAg and the electrochemical characterization for oxygen reduction reaction (ORR) in acid medium. The catalyst is obtained from the reduction of Pd(NO{sub 3}){sub 2}·2H{sub 2}O and AgNO{sub 3} with NaBH{sub 4} in THF. The synthesized compound was physically characterized with transmission electron microscopy (TEM), sweep electron microscopy (SEM) and x-ray diffraction (XRD) of powder. Electrochemical studies were conducted to determine the catalytic activity and intrinsic properties of the PdAg material for the ORR in acid medium using cyclic voltamperometry (CV), rotary disc electrode (RDE) and electrochemical impedance spectroscopy (EIS) in a solution of H{sub 2}SO{sub 4} 0.5 M at 25 degrees Celsius. The electrochemical current-potential responses were compared to those of palladium and platinum. The kinetic results showed an increase in the performance of the bimetallic electrocatalyst containing Ag as compared to pure Pd, but less than that obtained with nanometric Pt. The Tafel slopes obtained are roughly120 mV dec-1, similar to that reported for Pt and Pd and for other Ru-based electrocatalysts. [Spanish] En este trabajo se presentan la sintesis del compuesto binario PdAg y su caracterizacion electroquimica para la reaccion de reduccion de oxigeno (RRO) en medio acido. El catalizador se obtuvo a partir de la reduccion de Pd(NO{sub 3}){sub 2}·2H{sub 2}O y AgNO{sub 3} con NaBH{sub 4} en THF. El compuesto sintetizado se caracterizo fisicamente por microscopia electronica de transmision (MET), microscopia electronica de barrido (MEB) y difraccion de rayos X (DRX) de polvos. Se realizaron estudios electroquimicos para determinar la actividad catalitica y las propiedades intrinsecas del material de PdAg para la reaccion de reduccion de oxigeno (RRO) en medio acido, utilizando las tecnicas de voltamperometria ciclica (VC), electrodo disco rotatorio (EDR) y espectroscopia de impedancia electroquimica (EIE), en

  9. 气相色谱仪测定血液中DHA、EPA含量%Gas Chromatograph to Determine Content of DHA and EPA in the Blood

    Institute of Scientific and Technical Information of China (English)

    冯剑军

    2014-01-01

    二十二碳六烯酸(DHA)、二十碳五烯酸(EPA)是人体血液中的两种物质,对人体的健康有着重要的影响.因此,使用科学方法对血液中DHA,EPA进行检测是非常重要的.使用气相色谱仪对血液中的DHA和EPA进行检测实验,详细介绍了实验材料、方法和结果,最终证实,气相色谱仪适用于检测血液中的DHA和EPA.

  10. Extraction and concentration of DHA single cell oil%DHA单细胞油脂的萃取与浓缩

    Institute of Scientific and Technical Information of China (English)

    张娟梅; 柯崇榕; 黄建忠

    2008-01-01

    裂殖壶茵Schizochytrium sp.FJU-512细胞内富含生理活性物质二十二碳六烯酸(DHA),极具开发潜力.采用超临界CO2萃取技术对Schizochytrium sp.FJU-512 DHA单细胞油脂进行萃取,结合尿素包合法对所得单细胞油脂中的DHA进行浓缩.通过工艺优化,得到最佳提取条件为:萃取压力45 MPa,萃取温度35℃,萃取时间3 h,在该条件下单细胞油脂的得率高达25.9%;最佳浓缩工艺为:包合温度4 ℃,脂肪酸乙酯、尿素与乙醇的配比为1:2:12,包合时间6 h,该条件下DHA含量由46.1%提高到85.6%.

  11. Hippocampal Lipid Homeostasis in APP/PS1 Mice is Modulated by a Complex Interplay Between Dietary DHA and Estrogens: Relevance for Alzheimer's Disease.

    Science.gov (United States)

    Díaz, Mario; Fabelo, Noemí; Casañas-Sánchez, Verónica; Marin, Raquel; Gómez, Tomás; Quinto-Alemany, David; Pérez, José A

    2015-01-01

    Current evidence suggests that lipid homeostasis in the hippocampus is affected by different genetic, dietary, and hormonal factors, and that its deregulation may be associated with the onset and progression of Alzheimer's disease (AD). However, the precise levels of influence of each of these factors and their potential interactions remain largely unknown, particularly during neurodegenerative processes. In the present study, we have performed multifactorial analyses of the combined effects of diets containing different doses of docosahexaenoic acid (DHA), estrogen status (ovariectomized animals receiving vehicle or 17β-estradiol), and genotype (wild-type or transgenic APP/PS1 mice) in hippocampal lipid profiles. We have observed that the three factors affect lipid classes and fatty acid composition to different extents, and that strong interactions between these factors exist. The most aberrant lipid profiles were observed in APP/PS1 animals receiving DHA-poor diets and deprived of estrogens. Conversely, wild-type animals under a high-DHA diet and receiving estradiol exhibited a lipid profile that closely resembled that of the hippocampus of control animals. Interestingly, though the lipid signatures of APP/PS1 hippocampi markedly differed from wild-type, administration of a high-DHA diet in the presence of estrogens gave rise to a lipid profile that approached that of control animals. Paralleling changes in lipid composition, patterns of gene expression of enzymes involved in lipid biosynthesis were also altered and affected by combination of experimental factors. Overall, these results indicate that hippocampal lipid homeostasis is strongly affected by hormonal and dietary conditions, and that manipulation of these factors might be incorporated in AD therapeutics.

  12. DHA-Containing Oilseed: A Timely Solution for the Sustainability Issues Surrounding Fish Oil Sources of the Health-Benefitting Long-Chain Omega-3 Oils

    OpenAIRE

    Soressa M. Kitessa; Mahinda Abeywardena; Chakra Wijesundera; Nichols, Peter D.

    2014-01-01

    Benefits of long-chain (≥C20) omega-3 oils (LC omega-3 oils) for reduction of the risk of a range of disorders are well documented. The benefits result from eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA); optimal intake levels of these bioactive fatty acids for maintenance of normal health and prevention of diseases have been developed and adopted by national and international health agencies and science bodies. These developments have led to increased consumer demand for LC omega...

  13. Incorporation of EPA and DHA into plasma phospholipids in response to different omega-3 fatty acid formulations - a comparative bioavailability study of fish oil vs. krill oil

    OpenAIRE

    Schuchardt Jan; Schneider Inga; Meyer Henrike; Neubronner Juliane; von Schacky Clemens; Hahn Andreas

    2011-01-01

    Abstract Background Bioavailability of omega-3 fatty acids (FA) depends on their chemical form. Superior bioavailability has been suggested for phospholipid (PL) bound omega-3 FA in krill oil, but identical doses of different chemical forms have not been compared. Methods In a double-blinded crossover trial, we compared the uptake of three EPA+DHA formulations derived from fish oil (re-esterified triacylglycerides [rTAG], ethyl-esters [EE]) and krill oil (mainly PL). Changes of the FA composi...

  14. Stress Response and Recovery in Atlantic Salmon (Salmo salar L.) Fed Diets Low in the Marine n-3 Fatty Acids EPA and DHA

    OpenAIRE

    Cai, Chan

    2014-01-01

    With the fast growth of today's aquaculture industry, the demand for aquafeeds is expanding dramatically. Finding sustainable alternative sources to fishmeal and fish oil (FO) is becoming an urgent issue; vegetable oil (VO) and plant meal are potential candidates. Replacing the fishmeal and FO with plant materials means fish eat low levels of polyunsaturated fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA); this may affect the fish growth and health such as s...

  15. Long-chain omega-3 fatty acids and the brain: a review of the independent and shared effects of EPA, DPA and DHA

    OpenAIRE

    Dyall, Simon C.

    2015-01-01

    Omega-3 polyunsaturated fatty acids (PUFAs) exhibit neuroprotective properties and represent a potential treatment for a variety of neurodegenerative and neurological disorders. However, traditionally there has been a lack of discrimination between the different omega-3 PUFAs and effects have been broadly accredited to the series as a whole. Evidence for unique effects of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and more recently docosapentaenoic acid (DPA) is growing. For exam...

  16. DHA和EPA的生理作用及开发利用研究进展%A Summary of DHA's and EPA's Special Physiological Functions and Their Exploitations

    Institute of Scientific and Technical Information of China (English)

    张春艳

    2005-01-01

    二十二碳六烯酸(DHA)和二十碳五烯酸(EPA),两者常混存于食物中,特别是海产鱼类的鱼油中.对人的大脑、心血管、人体免疫力等有特殊的作用和影响.本文综述了DHA、EPA的代谢机理、生理作用及开发利用.

  17. EPA and DHA exposure alters the inflammatory response but not the surface expression of toll-like receptor 4 in macrophages

    OpenAIRE

    Honda, Kaori L.; Lamon-Fava, Stefania; Matthan, Nirupa R.; Wu, Dayong; Lichtenstein, Alice H

    2014-01-01

    Dietary intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and their respective enrichment in cell membranes have been negatively associated with atherosclerotic lesion development. This effect may be mediated, in part, by dampened inflammatory response of macrophages triggered by toll-like receptor 4 (TLR4) activation. This study investigated the influence of membrane fatty acid profile on TLR4-mediated inflammation in RAW 264.7 macrophages. Cells pretreated with myristic a...

  18. Enhanced anti-inflammatory effects of DHA and quercetin in lipopolysaccharide-induced RAW264.7 macrophages by inhibiting NF-κB and MAPK activation.

    Science.gov (United States)

    Si, Tian-Lei; Liu, Qi; Ren, Yu-Fei; Li, Hui; Xu, Xiao-Yun; Li, Er-Hu; Pan, Si-Yi; Zhang, Jiu-Liang; Wang, Ke-Xing

    2016-07-01

    The aim of the present study was to investigate the anti-inflammatory effects of docosahexaenoic acid (DHA) + quercetin (QE) used in combination. DHA and QE are natural compounds derived from various foods and have been demonstrated to exert anti‑inflammatory effects The protein mRNA expression involved in the nuclear factor (NF)-κB and mitogen-activated protein kinase (MAPK) signalling pathway was analyzed by western blot analysis and reverse transcription-polymerase chain reaction methods respectively, other cytokines were detected by an enzyme‑linked immunosorbent assay kit. The results of the present study demonstrated that combined treatment of lipopolysaccharide (LPS)‑stimulated RAW264.7 cells with DHA + QE decreased the levels of pro‑inflammatory mediators to a greater extent than QE or DHA alone. Additionally, DHA + QE synergistically suppressed nitric oxide, prostaglandin E2 and cyclooxygenase-2 levels. Molecular‑level studies indicated that the DHA + QE combination can significantly inhibit the mRNA expression of NF‑κB subunits p50 and p65, extracellular signal‑regulated kinase (ERK) 1/2 and c‑JUN N‑terminal kinase (JNK) 1/2, which suggests that the NF‑κB signalling pathway is involved in the synergistic effects observed. Furthermore, western blot analysis demonstrated that DHA + QE synergistically inhibit the phosphorylation of p50, p65, ERK1/2 and JNK1/2. This finding indicates that the enhanced anti‑inflammatory effects of the combined compounds are achieved by suppressing NF‑κB and MAPK signalling in LPS‑stimulated RAW264.7 cells. The results of the present study suggest that DHA and QE in combination may be utilized as potent anti‑inflammatory compounds, with potential preventative or palliative effects on obesity, atherosclerosis and cardiovascular diseases. PMID:27176922

  19. Reduction of n-3 PUFAs, specifically DHA and EPA, and enhancement of peroxisomal beta-oxidation in type 2 diabetic rat heart

    Directory of Open Access Journals (Sweden)

    Hou Lianguo

    2012-10-01

    Full Text Available Abstract Background There is overwhelming evidence that dietary supplementation with n-3 polyunsaturated fatty acids (PUFAs, mainly EPA (C20:5n-3 and DHA (C22:6n-3, has cardiovascular protective effects on patients with type 2 diabetes mellitus (T2DM but not on healthy people. Because the T2DM heart increases fatty acid oxidation (FAO to compensate for the diminished utilization of glucose, we hypothesize that T2DM hearts consume more n-3 PUFAs and, therefore, need more n-3 PUFAs. In the present study, we investigated the changes in cardiac n-3 PUFAs and peroxisomal beta-oxidation, which are responsible for the degradation of PUFAs in a high-fat diet (HFD and low-dose streptozotocin- (STZ induced type 2 diabetic rat model. Methods and results The capillary gas chromatography results showed that all the n-3 (or omega-3 PUFAs, especially DHA (~50% and EPA (~100%, were significantly decreased, and the n-6/n-3 ratio (~115% was significantly increased in the hearts of diabetic rats. The activity of peroxisomal beta-oxidation, which is crucial to very-long-chain and unsaturated FA metabolism (including DHA, was significantly elevated in DM hearts. Additionally, the real-time PCR results showed that the mRNA expression of most peroxisomal beta-oxidation key enzymes were up-regulated in T2DM rat hearts, which might contribute to the reduction of n-3 (or omega-3 PUFAs. Conclusion In conclusion, our results indicate that T2DM hearts consume more n-3 PUFAs, especially DHA and EPA, due to exaggerated peroxisomal beta-oxidation.

  20. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the extension of use for DHA and EPA-rich algal oil from Schizochytrium sp. as a Novel Food ingredient

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on an extension of use for docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)-rich algal oil from Schizochytrium sp. as a novel food...... ingredient (NFI) in the context of Regulation (EC) No 258/97. The NFI is already authorised for use in a range of foodstuffs at specified maximum levels. The applicant requests an extension of use of the NFI in food supplements up to a maximum DHA and EPA content of 3 g per daily dose for the adult...... population, excluding pregnant and lactating women. In a previous opinion on the Tolerable Upper Intake Level of EPA, DHA and docosapentaenoic acid (DPA), the Panel concluded that supplemental intake of EPA and DHA combined at doses up to 5 g/day, does not give rise to safety concerns for adults. Based on...

  1. Identification of a novel C22-∆4-producing docosahexaenoic acid (DHA) specific polyunsaturated fatty acid desaturase gene from Isochrysis galbana and its expression in Saccharomyces cerevisiae.

    Science.gov (United States)

    Shi, Tonglei; Yu, Aiqun; Li, Ming; Ou, Xiuyuan; Xing, Laijun; Li, Mingchun

    2012-12-01

    Isochrysis galbana, produces long chain polyunsaturated fatty acids including docosahexaenoic acid (DHA, 22:6n-3). A novel gene (IgFAD4-2), encoding a C22-∆4 polyunsaturated fatty acid specific desaturase, has been isolated and characterized from I. galbana. A full-length cDNA of 1,302 bp was cloned by LA-PCR technique. The IgFAD4-2 encoded a protein of 433 amino acids that shares 78 % identity with a previously reported ∆4-desaturase (IgFAD4-1) from I. galbana. The function of IgFAD4-2 was deduced by its heterologous expression in Saccharomyces cerevisiae, which then desaturated docosapentaenoic acid (DPA, 22:5n-3) to DHA. The conversion ratio of DPA to DHA was 34 %, which is higher than other ∆4-desaturases cloned from algae. However, IgFAD4-2 did not catalyze the desaturation or elongation reactions with other fatty acids. These results confirm that IgFAD4-2 has C22-∆4-PUFAs-specific desaturase activity.

  2. DHA Supplementation Alone or in Combination with Other Nutrients Does not Modulate Cerebral Hemodynamics or Cognitive Function in Healthy Older Adults

    Directory of Open Access Journals (Sweden)

    Philippa A. Jackson

    2016-02-01

    Full Text Available A number of recent trials have demonstrated positive effects of dietary supplementation with the omega-3 polyunsaturated fatty acids (n-3 PUFAs, docosahexaenoic acid (DHA and eicosapentaenoic acid (EPA on measures of cognitive function in healthy young and older adults. One potential mechanism by which EPA, and DHA in particular, may exert these effects is via modulation of cerebral hemodynamics. In order to investigate the effects of DHA alone or provided as one component of a multinutrient supplement (also including Gingko biloba, phosphatidylserine and vitamins B9 and B12 on measures of cerebral hemodynamics and cognitive function, 86 healthy older adults aged 50–70 years who reported subjective memory deficits were recruited to take part in a six month daily dietary supplementation trial. Relative changes in the concentration of oxygenated hemoglobin and deoxygenated hemoglobin were assessed using Near Infrared Spectroscopy (NIRS during the performance of cognitive tasks prior to and following the intervention period. Performance on the cognitive tasks was also assessed. No effect of either active treatment was found for any of the NIRS measures or on the cognitive performance tasks, although the study was limited by a number of factors. Further work should continue to evaluate more holistic approaches to cognitive aging.

  3. Les microalgues marines : source alternative d’acide eicosapentaènoïque (EPA et d’acide docosahexaènoïque (DHA

    Directory of Open Access Journals (Sweden)

    Pencreac’h Gaëlle

    2004-03-01

    Full Text Available Les lipides de microalgues marines sont riches en acide eicosapentaènoïque (EPA, C20 :5 et en acide docosahexaènoïque (DHA, C22 :6, deux acides gras hautement insaturés de la série ω3 (AGHI-ω3, et représentent aujourd’hui une source alternative potentielle face aux huiles de poissons. Les microalgues synthétisent de l’EPA et\\\\ou du DHA dans des proportions relatives variables selon leur classe taxonomique. D’autre part, pour une même espèce, les teneurs en ces acides gras dépendent fortement des conditions de culture. De part leur métabolisme photoautotrophe, la production de biomasse microalgale à grande échelle nécessite la conception de bioréacteurs spécifiques, les photobioréacteurs, qui représentent encore aujourd’hui un défi technologique. La poursuite de travaux de recherche, dans des domaines très divers, reste nécessaire pour confirmer la viabilité économique des procédés industriels de production d’EPA et de DHA par les microalgues.

  4. A SURVEY ON EPA & DHA IN PUFFERFISH TISSUES%六种河豚鱼组织中EPA与DHA含量调查

    Institute of Scientific and Technical Information of China (English)

    张风雷; 赖少阳; 戴桂勋; 王臻

    2004-01-01

    目的:分析河豚鱼组织中EPA(二十碳五烯酸)、DHA(二十二碳六烯酸)含量及其利用价值.方法:利用带FID检测器的气相色谱仪作为检测设备,取河豚鱼的肌肉、肝脏进行EPA、DHA定量测定.结果:河豚鱼肌肉未能检出EPA、DHA;肝脏含EPA范围0.036~7.800 g/kg,含DHA范围0.030~45.672 g/kg;东方豚肝脏中EPA、DHA含量显著高于腹刺豚;无毒的棕腹刺豚和暗鳍腹刺豚肝脏内的EPA、DHA含量差异有统计学意义.结论:河豚鱼肝脏含有丰富的EPA、DHA.棕腹刺豚肝脏没有毒性,是摄取EPA、DHA的可利用资源.

  5. Changes of arterial blood pressure, heart rate, internal body temperature, and blood acido-basic balance in the unanaesthetized rabbit following whole-body gamma irradiation at a mean absorbed dose of 250 rads

    Energy Technology Data Exchange (ETDEWEB)

    Dufour, R.; Court, L.

    1973-09-01

    The general effects of whole-body gamma -irradiation at a mean absorbed dose of 250 rads were studied simultaneously in the unanaesthetized rabbit for 48 hours. They occurred early, with the following characteristics: arterial blood pressure decreased steadily as early as the 2nd hour and reached its minimum value on the 5th hour with a decrease of about 14%; it remained low during the following two days. Heart rate increased during the first hour, was the highest by the end of the second hour, and resumed normal value on the 24th hour. Internal body temperature increased during the 1st hour and was maximum by the end of the 2nd hour, with a mean increase of 1.2 deg C; hyperthermia steadily decreased between the 4th and the 6th hours and had completely disappeared by the 24th hour. Respiratory alkalosis is shown in the acido-basic balance by a raise of pH, a decrease of PCO/sub 2/ and arterial blood bicarbonates. These various changes seem to indicate a double origin, both central and peripheral. (FR)

  6. DHA single cell oil:DHA supplement for infant,pregnant and lactation woman%二十二碳六烯酸单细胞油(DHASCO)--婴幼儿及孕产妇的DHA营养补充剂

    Institute of Scientific and Technical Information of China (English)

    陈亚非; 黄珮珊

    2005-01-01

    DHA是大脑中最丰富的长链多不饱和脂肪酸之一,也是视网膜光受体中最丰富的多不饱和脂肪酸.然而人体,特别是婴幼儿自身合成DHA的能力有限,缺乏DHA会影响大脑和视力的发育及其功能,故从饮食中摄取足够的DHA十分重要.鱼油是目前DHA的主要来源,但鱼油中与DHA同时存在的EPA会影响婴儿的生长发育,因此鱼油不适合添加进婴幼儿配方食品中.利用现代生物技术生产的二十二碳六烯酸单细胞油含有40%左右的高纯度DHA,几乎不含EPA,产品已能工业化生产,各种理化指标稳定,纯度和安全性优于鱼油,目前已开始应用于婴幼儿奶粉和作为功能性食品原料使用.评述了二十二碳六烯酸单细胞油的原料来源、制作过程、理化性状、吸收代谢、安全性以及在婴幼儿、孕产妇的临床功能研究等方面的研究进展,并对二十二碳六烯酸单细胞油在保健食品中的应用情况作了简单的介绍.

  7. Robust optimization for nonlinear time-delay dynamical system of dha regulon with cost sensitivity constraint in batch culture

    Science.gov (United States)

    Yuan, Jinlong; Zhang, Xu; Liu, Chongyang; Chang, Liang; Xie, Jun; Feng, Enmin; Yin, Hongchao; Xiu, Zhilong

    2016-09-01

    Time-delay dynamical systems, which depend on both the current state of the system and the state at delayed times, have been an active area of research in many real-world applications. In this paper, we consider a nonlinear time-delay dynamical system of dha-regulonwith unknown time-delays in batch culture of glycerol bioconversion to 1,3-propanediol induced by Klebsiella pneumonia. Some important properties and strong positive invariance are discussed. Because of the difficulty in accurately measuring the concentrations of intracellular substances and the absence of equilibrium points for the time-delay system, a quantitative biological robustness for the concentrations of intracellular substances is defined by penalizing a weighted sum of the expectation and variance of the relative deviation between system outputs before and after the time-delays are perturbed. Our goal is to determine optimal values of the time-delays. To this end, we formulate an optimization problem in which the time delays are decision variables and the cost function is to minimize the biological robustness. This optimization problem is subject to the time-delay system, parameter constraints, continuous state inequality constraints for ensuring that the concentrations of extracellular and intracellular substances lie within specified limits, a quality constraint to reflect operational requirements and a cost sensitivity constraint for ensuring that an acceptable level of the system performance is achieved. It is approximated as a sequence of nonlinear programming sub-problems through the application of constraint transcription and local smoothing approximation techniques. Due to the highly complex nature of this optimization problem, the computational cost is high. Thus, a parallel algorithm is proposed to solve these nonlinear programming sub-problems based on the filled function method. Finally, it is observed that the obtained optimal estimates for the time-delays are highly satisfactory

  8. Continuous gradient temperature Raman spectroscopy of the long chain polyunsaturated fatty acids docosapentaenoic (DPA, 22:5n-6) and docosahexaenoic (DHA; 22:6n-3) from -100 to 20° C

    Science.gov (United States)

    Broadhurst, C. Leigh; Schmidt, Walter F.; Kim, Moon S.; Nguyen, Julie K.; Qin, Jianwei; Chao, Kuanglin; Bauchan, Gary L.; Shelton, Daniel R.

    2016-05-01

    The structural, cognitive and visual development of the human brain and retina strictly require long-chain polyunsaturated fatty acids (LC-PUFA). Excluding water, the mammalian brain is about 60% lipid. One of the great unanswered questions with respect to biological science in general is the absolute necessity of the LC-PUFA docosahexaenoic acid (DHA; 22:6n-3) in these fast signal processing tissues. A lipid of the same chain length with just one less diene group, docosapentaenoic acid (DPA; 22:5n-6) is fairly abundant in terrestrial food chains yet cannot substitute for DHA. Gradient Temperature Raman spectroscopy (GTRS) applies the temperature gradients utilized in differential scanning calorimetry to Raman spectroscopy, providing a straightforward technique to identify molecular rearrangements that occur near and at phase transitions. Herein we apply GTRS to DPA, and DHA from -100 to 20°C. 20 Mb three-dimensional data arrays with 1°C increments and first/second derivatives allows complete assignment of solid, liquid and transition state vibrational modes, including low intensity/frequency vibrations that cannot be readily analyzed with conventional Raman. DPA and DHA show significant spectral changes with premelting (-33 and -60°C, respectively) and melting (-27 and -44°C, respectively). The CH2-(HC=CH)-CH2 moieties are not identical in the second half of the DHA and DPA structures. The DHA molecule contains major CH2 twisting (1265 cm-1) with no noticeable CH2 bending, consistent with a flat helical structure with small pitch. Further modeling of neuronal membrane phospholipids must take into account this structure for DHA, which would be configured parallel to the hydrophilic head group line.

  9. Gene expression of fatty acid transport and binding proteins in the blood-brain barrier and the cerebral cortex of the rat: differences across development and with different DHA brain status.

    Science.gov (United States)

    Pélerin, Hélène; Jouin, Mélanie; Lallemand, Marie-Sylvie; Alessandri, Jean-Marc; Cunnane, Stephen C; Langelier, Bénédicte; Guesnet, Philippe

    2014-11-01

    Specific mechanisms for maintaining docosahexaenoic acid (DHA) concentration in brain cells but also transporting DHA from the blood across the blood-brain barrier (BBB) are not agreed upon. Our main objective was therefore to evaluate the level of gene expression of fatty acid transport and fatty acid binding proteins in the cerebral cortex and at the BBB level during the perinatal period of active brain DHA accretion, at weaning, and until the adult age. We measured by real time RT-PCR the mRNA expression of different isoforms of fatty acid transport proteins (FATPs), long-chain acyl-CoA synthetases (ACSLs), fatty acid binding proteins (FABPs) and the fatty acid transporter (FAT)/CD36 in cerebral cortex and isolated microvessels at embryonic day 18 (E18) and postnatal days 14, 21 and 60 (P14, P21 and P60, respectively) in rats receiving different n-3 PUFA dietary supplies (control, totally deficient or DHA-supplemented). In control rats, all the genes were expressed at the BBB level (P14 to P60), the mRNA levels of FABP5 and ACSL3 having the highest values. Age-dependent differences included a systematic decrease in the mRNA expressions between P14-P21 and P60 (2 to 3-fold), with FABP7 mRNA abundance being the most affected (10-fold). In the cerebral cortex, mRNA levels varied differently since FATP4, ACSL3 and ACSL6 and the three FABPs genes were highly expressed. There were no significant differences in the expression of the 10 genes studied in n-3 deficient or DHA-supplemented rats despite significant differences in their brain DHA content, suggesting that brain DHA uptake from the blood does not necessarily require specific transporters within cerebral endothelial cells and could, under these experimental conditions, be a simple passive diffusion process. PMID:25123062

  10. Nutritional Evaluation of an EPA-DHA Oil from Transgenic Camelina sativa in Feeds for Post-Smolt Atlantic Salmon (Salmo salar L..

    Directory of Open Access Journals (Sweden)

    Mónica B Betancor

    Full Text Available Vegetable oils (VO are possible substitutes for fish oil in aquafeeds but their use is limited by their lack of omega-3 (n-3 long-chain polyunsaturated fatty acids (LC-PUFA. However, oilseed crops can be modified to produce n-3 LC-PUFA such as eicosapentaenoic (EPA and docosahexaenoic (DHA acids, representing a potential option to fill the gap between supply and demand of these important nutrients. Camelina sativa was metabolically engineered to produce a seed oil with around 15% total n-3 LC-PUFA to potentially substitute for fish oil in salmon feeds. Post-smolt Atlantic salmon (Salmo salar were fed for 11-weeks with one of three experimental diets containing either fish oil (FO, wild-type Camelina oil (WCO or transgenic Camelina oil (DCO as added lipid source to evaluate fish performance, nutrient digestibility, tissue n-3 LC-PUFA, and metabolic impact determined by liver transcriptome analysis. The DCO diet did not affect any of the performance or health parameters studied and enhanced apparent digestibility of EPA and DHA compared to the WCO diet. The level of total n-3 LC-PUFA was higher in all the tissues of DCO-fed fish than in WCO-fed fish with levels in liver similar to those in fish fed FO. Endogenous LC-PUFA biosynthetic activity was observed in fish fed both the Camelina oil diets as indicated by the liver transcriptome and levels of intermediate metabolites such as docosapentaenoic acid, with data suggesting that the dietary combination of EPA and DHA inhibited desaturation and elongation activities. Expression of genes involved in phospholipid and triacylglycerol metabolism followed a similar pattern in fish fed DCO and WCO despite the difference in n-3 LC-PUFA contents.

  11. Nutritional Evaluation of an EPA-DHA Oil from Transgenic Camelina sativa in Feeds for Post-Smolt Atlantic Salmon (Salmo salar L.).

    Science.gov (United States)

    Betancor, Mónica B; Sprague, Matthew; Sayanova, Olga; Usher, Sarah; Metochis, Christoforos; Campbell, Patrick J; Napier, Johnathan A; Tocher, Douglas R

    2016-01-01

    Vegetable oils (VO) are possible substitutes for fish oil in aquafeeds but their use is limited by their lack of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA). However, oilseed crops can be modified to produce n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, representing a potential option to fill the gap between supply and demand of these important nutrients. Camelina sativa was metabolically engineered to produce a seed oil with around 15% total n-3 LC-PUFA to potentially substitute for fish oil in salmon feeds. Post-smolt Atlantic salmon (Salmo salar) were fed for 11-weeks with one of three experimental diets containing either fish oil (FO), wild-type Camelina oil (WCO) or transgenic Camelina oil (DCO) as added lipid source to evaluate fish performance, nutrient digestibility, tissue n-3 LC-PUFA, and metabolic impact determined by liver transcriptome analysis. The DCO diet did not affect any of the performance or health parameters studied and enhanced apparent digestibility of EPA and DHA compared to the WCO diet. The level of total n-3 LC-PUFA was higher in all the tissues of DCO-fed fish than in WCO-fed fish with levels in liver similar to those in fish fed FO. Endogenous LC-PUFA biosynthetic activity was observed in fish fed both the Camelina oil diets as indicated by the liver transcriptome and levels of intermediate metabolites such as docosapentaenoic acid, with data suggesting that the dietary combination of EPA and DHA inhibited desaturation and elongation activities. Expression of genes involved in phospholipid and triacylglycerol metabolism followed a similar pattern in fish fed DCO and WCO despite the difference in n-3 LC-PUFA contents. PMID:27454884

  12. Carbapenem and cefoxitin resistance of Klebsiella pneumoniae strains associated with porin OmpK36 loss and DHA-1 β-lactamase production

    Directory of Open Access Journals (Sweden)

    Weifeng Shi

    2013-01-01

    Full Text Available Clinical isolates of carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae strains are being increased worldwide. Five pan-resistant K. pneumoniae strains have been isolated from respiratory and ICU wards in a Chinese hospital, and reveal strong resistance to all β-lactams, fluoroquinolones and aminoglycosides. Totally 27 β-lactamase genes and 2 membrane pore protein (porin genes in 5 K. pneumoniae strains were screened by polymerase chain reaction (PCR. The results indicated that all of 5 K. pneumoniae strains carried blaTEM-1 and blaDHA-1 genes, as well as base deletion and mutation of OmpK35 or OmpK36 genes. Compared with carbapenem-sensitive isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, the resistant isolates markedly lacked the protein band of 34-40 kDa, which might be the outer membrane proteins of OmpK36 according to the electrophoresis mobility. In addition, the conjugation test was confirmed that blaDHA-1 mediated by plasmids could be transferred between resistant and sensitive strains. When reserpine (30 µg/mL and carbonyl cyanide m-chlorophenylhydrazone (CCCP (50 µg/mL were added in imipenem and meropenem, the MICs had no change against K. pneumoniae strains. These results suggest that both DHA-1 β-lactamase and loss or deficiency of porin OmpK36 may be the main reason for the cefoxitin and carbapenem resistance in K. pneumoniae strains in our hospital.

  13. Progressive retinal degeneration and glial activation in the CLN6 (nclf mouse model of neuronal ceroid lipofuscinosis: a beneficial effect of DHA and curcumin supplementation.

    Directory of Open Access Journals (Sweden)

    Myriam Mirza

    Full Text Available Neuronal ceroid lipofuscinosis (NCL is a group of neurodegenerative lysosomal storage disorders characterized by vision loss, mental and motor deficits, and spontaneous seizures. Neuropathological analyses of autopsy material from NCL patients and animal models revealed brain atrophy closely associated with glial activity. Earlier reports also noticed loss of retinal cells and reactive gliosis in some forms of NCL. To study this phenomenon in detail, we analyzed the ocular phenotype of CLN6 (nclf mice, an established mouse model for variant-late infantile NCL. Retinal morphometry, immunohistochemistry, optokinetic tracking, electroretinography, and mRNA expression were used to characterize retinal morphology and function as well as the responses of Müller cells and microglia. Our histological data showed a severe and progressive degeneration in the CLN6 (nclf retina co-inciding with reactive Müller glia. Furthermore, a prominent phenotypic transformation of ramified microglia to phagocytic, bloated, and mislocalized microglial cells was identified in CLN6 (nclf retinas. These events overlapped with a rapid loss of visual perception and retinal function. Based on the strong microglia reactivity we hypothesized that dietary supplementation with immuno-regulatory compounds, curcumin and docosahexaenoic acid (DHA, could ameliorate microgliosis and reduce retinal degeneration. Our analyses showed that treatment of three-week-old CLN6 (nclf mice with either 5% DHA or 0.6% curcumin for 30 weeks resulted in a reduced number of amoeboid reactive microglia and partially improved retinal function. DHA-treatment also improved the morphology of CLN6 (nclf retinas with a preserved thickness of the photoreceptor layer in most regions of the retina. Our results suggest that microglial reactivity closely accompanies disease progression in the CLN6 (nclf retina and both processes can be attenuated with dietary supplemented immuno-modulating compounds.

  14. Results of test of acid fluids neutralization in the well H-43, Los Humeros geothermal field, Puebla; Resultados de la prueba de neutralizacion de fluidos acidos en el pozo H-43, campo geotermico de Los Humeros, Puebla

    Energy Technology Data Exchange (ETDEWEB)

    Flores Armenta, Magaly del Carmen; Ramirez Montes, Miguel; Sandoval Medina, Fernando; Rosales Lopez, Cesar [Comision Federal de Electricidad, Gerencia de Proyectos Geotermoelectricos, Morelia, Michoacan (Mexico)]. E-mail: magaly.flores@cfe.gob.mx

    2011-07-15

    The well H-43 was drilled in Los Humeros Geothermal Field, Pue., in 2007 and 2008. When well production was measured, it was found the well produced acid fluids with high corrosion potential. Then it was decided to try to neutralize the acidity of the H-43 fluids by adding a solution of sodium hydroxide. This is a basic substance used to neutralize mainly the acid groups H+, and the goal was to raise the pH of the fluids to minimize its corrosive features. First sodium hydroxide was injected into the well to protect the casing of 244.4 mm (9 5/8 in) against corrosion and then all the surface installations. It was possible to increase the pH of the well fluid from 5.2 up to 6.8 without altering the steam production, thus demonstrating the neutralization procedure is feasible. The approximate costs of the neutralization tests performed, including equipment for the proposed neutralization system, the neutralizer (NaOH), and drilling the well are about 71.4 million pesos (around 5.5 million USD). We estimate a benefit/cost ratio of 1.5 and a return on investment in five years, considering the income from energy sales at present value. [Spanish] El pozo H-43 fue perforado en el campo de Los Humeros, Pue., en 2007-2008, y al evaluar su produccion se encontro que producia fluidos acidos de alto potencial corrosivo. Se decidio por tanto realizar una prueba para neutralizar la acidez de los fluidos de este pozo, que consistio en agregarle una solucion de hidroxido de sodio. Esta es una sustancia basica que neutraliza principalmente los grupos acidos H+, con lo que se buscaba aumentar el pH del fluido lo necesario para reducir al maximo su caracter corrosivo. La inyeccion del hidroxido de sodio se realizo dentro del pozo a fin de proteger de la corrosion a la tuberia de revestimiento de 244.4 mm (9 5/8 pulgadas), y posteriormente a todos los equipos superficiales. Como resultado de la prueba, se logro aumentar el pH del fluido producido por el pozo de 5.2 a un maximo de 6

  15. Reduction of n-3 PUFAs, specifically DHA and EPA, and enhancement of peroxisomal beta-oxidation in type 2 diabetic rat heart

    OpenAIRE

    Hou Lianguo; Lian Kaoqi; Yao Min; Shi Yun; Lu Xin; Fang Lijia; He Tianbo; Jiang Lingling

    2012-01-01

    Abstract Background There is overwhelming evidence that dietary supplementation with n-3 polyunsaturated fatty acids (PUFAs), mainly EPA (C20:5n-3) and DHA (C22:6n-3), has cardiovascular protective effects on patients with type 2 diabetes mellitus (T2DM) but not on healthy people. Because the T2DM heart increases fatty acid oxidation (FAO) to compensate for the diminished utilization of glucose, we hypothesize that T2DM hearts consume more n-3 PUFAs and, therefore, need more n-3 PUFAs. In the...

  16. Iron and a mixture of DHA and EPA supplementation, alone and in combination, affect bioactive lipid signalling and morbidity of iron deficient South African school children in a two-by-two randomised controlled trial.

    Science.gov (United States)

    Malan, L; Baumgartner, J; Zandberg, L; Calder, P C; Smuts, C M

    2016-02-01

    We recently reported that iron supplementation increased respiratory morbidity in iron deficient South African children. This increase, however, was attenuated when iron was provided in combination with a mixture of DHA/EPA. To explore potential underlying mechanisms, we examined the effects of iron and DHA/EPA, alone and in combination, on plasma lipid-derived immune modulator concentrations and related gene expression in peripheral blood mononuclear cells (PBMC). DHA/EPA decreased inflammatory 12-hydroxyeicosatetraenoic acid and tended to increase anti-inflammatory and pro-resolving 17-hydroxydocosahexaenoic acid (17-HDHA), while iron decreased 17-HDHA. However, in combination with iron, the anti-inflammatory effect of DHA/EPA was maintained. These biochemical changes may explain the prevention of iron-induced respiratory morbidity that we observed when iron was supplemented in combination with DHA/EPA during the 8.5 month randomised controlled trial and might lead to a safer approach of delivering iron supplementation. The study was registered at clinicaltrials.gov as NCT01092377.

  17. Cloning and sequencing of the plasmid-mediated DHA-1 gene of AmpC β-lactamase in Klebsiella pnuemoniae%肺炎克雷伯菌DHA-1型质粒AmpC酶全编码基因克隆、表达及序列分析

    Institute of Scientific and Technical Information of China (English)

    多丽波; 刘晶; 杨东晓; 王孟滨; 周虹

    2005-01-01

    目的以我院临床分离的肺炎克雷伯菌KP1为研究对象,对其所产生的质粒AmpC酶编码基因的序列进行研究. 方法采用聚合酶链反应(PCR),用DHA-1型AmpC酶引物扩增KP1接合子中AmpC酶全编码基因,并克隆到pUC118载体中进行表达,对PCR产物进行测序,分析其基因型别. 结果肺炎克雷伯菌KP1菌株的转移接合子所含有的质粒AmpC酶为DHA型,编码基因序列与GenBank中DHA-1编码基因序列的同源性为100%. 结论哈尔滨地区临床分离肺炎克雷伯菌有可转移性DHA-1型AmpC酶的存在.

  18. DHA-1型质粒AmpC酶调控因子AmpR基因检测%Detection of regulator AmpR genes of DHA-1 type plasmid-encoded AmpC β-lactamase in escherichia coli and klebsiella pnuemoniae isolates

    Institute of Scientific and Technical Information of China (English)

    多丽波; 栾英; 张联博; 李垚

    2007-01-01

    目的 检测DHA-1型质粒AmpC酶诱导性耐药调控因子AmpR基因.方法 以我院临床分离产DHA-1型质粒AmpC酶的4株大肠埃希菌和10株肺炎克雷伯菌为研究对象,采用聚合酶链反应(PCR),用特异性引物扩增AmpR调节因子全编码基因,并将一株肺炎克雷伯菌AmpR基因克隆到pET-22b(+)载体质粒中测序.结果 所有实验菌株AmpR基因检测阳性,重组子AmpR基因测序表明与摩根摩根菌染色体上AmpR基因同源性达99.8%.结论 产DHA-1型质粒AmpC酶肺炎克雷伯菌、大肠埃希菌的质粒上存在调控因子AmpR基因.

  19. Lipidomics and H218O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio) embryos

    Science.gov (United States)

    McDougall, Melissa Q.; Choi, Jaewoo; Stevens, Jan F.; Truong, Lisa; Tanguay, Robert L.; Traber, Maret G.

    2016-01-01

    We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without (E−) or with added α-tocopherol (E+, 500 mg RRR-α-tocopheryl acetate/kg diet) for a minimum of 80 days, and then spawned them to obtain E− and E+ embryos. The E− compared with E+ embryos were 82% less responsive (p<0.01) to a light/dark stimulus at 96 h post-fertilization (hpf), demonstrating impaired locomotor behavior, even in the absence of gross morphological defects. Evaluation of phospholipid (PL) and lysophospholipid (lyso-PL) composition using untargeted lipidomics in E− compared with E+ embryos at 24, 48, 72, and 120 hpf showed that four PLs and three lyso-PLs containing docosahexaenoic acid (DHA), including lysophosphatidylcholine (LPC 22:6, required for transport of DHA into the brain, p<0.001), were at lower concentrations in E− at all time-points. Additionally, H218O labeling experiments revealed enhanced turnover of LPC 22:6 (p<0.001) and three other DHA-containing PLs in the E− compared with the E+ embryos, suggesting that increased membrane remodeling is a result of PL depletion. Together, these data indicate that α-tocopherol deficiency in the zebrafish embryo causes the specific depletion and increased turnover of DHA-containing PL and lyso-PLs, which may compromise DHA delivery to the brain and thereby contribute to the functional impairments observed in E− embryos. PMID:26774753

  20. Lipidomics and H218O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio embryos

    Directory of Open Access Journals (Sweden)

    Melissa Q. McDougall

    2016-08-01

    Full Text Available We hypothesized that vitamin E (α-tocopherol is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6, the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio defined diets without (E− or with added α-tocopherol (E+, 500 mg RRR-α-tocopheryl acetate/kg diet for a minimum of 80 days, and then spawned them to obtain E− and E+ embryos. The E− compared with E+ embryos were 82% less responsive (p<0.01 to a light/dark stimulus at 96 h post-fertilization (hpf, demonstrating impaired locomotor behavior, even in the absence of gross morphological defects. Evaluation of phospholipid (PL and lysophospholipid (lyso-PL composition using untargeted lipidomics in E− compared with E+ embryos at 24, 48, 72, and 120 hpf showed that four PLs and three lyso-PLs containing docosahexaenoic acid (DHA, including lysophosphatidylcholine (LPC 22:6, required for transport of DHA into the brain, p<0.001, were at lower concentrations in E− at all time-points. Additionally, H218O labeling experiments revealed enhanced turnover of LPC 22:6 (p<0.001 and three other DHA-containing PLs in the E− compared with the E+ embryos, suggesting that increased membrane remodeling is a result of PL depletion. Together, these data indicate that α-tocopherol deficiency in the zebrafish embryo causes the specific depletion and increased turnover of DHA-containing PL and lyso-PLs, which may compromise DHA delivery to the brain and thereby contribute to the functional impairments observed in E− embryos.

  1. Lipidomics and H2(18)O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio) embryos.

    Science.gov (United States)

    McDougall, Melissa Q; Choi, Jaewoo; Stevens, Jan F; Truong, Lisa; Tanguay, Robert L; Traber, Maret G

    2016-08-01

    We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without (E-) or with added α-tocopherol (E+, 500mg RRR-α-tocopheryl acetate/kg diet) for a minimum of 80 days, and then spawned them to obtain E- and E+ embryos. The E- compared with E+ embryos were 82% less responsive (p<0.01) to a light/dark stimulus at 96h post-fertilization (hpf), demonstrating impaired locomotor behavior, even in the absence of gross morphological defects. Evaluation of phospholipid (PL) and lysophospholipid (lyso-PL) composition using untargeted lipidomics in E- compared with E+ embryos at 24, 48, 72, and 120hpf showed that four PLs and three lyso-PLs containing docosahexaenoic acid (DHA), including lysophosphatidylcholine (LPC 22:6, required for transport of DHA into the brain, p<0.001), were at lower concentrations in E- at all time-points. Additionally, H2(18)O labeling experiments revealed enhanced turnover of LPC 22:6 (p<0.001) and three other DHA-containing PLs in the E- compared with the E+ embryos, suggesting that increased membrane remodeling is a result of PL depletion. Together, these data indicate that α-tocopherol deficiency in the zebrafish embryo causes the specific depletion and increased turnover of DHA-containing PL and lyso-PLs, which may compromise DHA delivery to the brain and thereby contribute to the functional impairments observed in E- embryos. PMID:26774753

  2. 贮藏条件对DHA微胶囊化学稳定性的影响研究%Influences of Storage Conditions on Chemical Stability of DHA Microcapsules

    Institute of Scientific and Technical Information of China (English)

    何伟; 郭华; 刘向宇; 邓敏

    2011-01-01

    This article studied the influences of storage conditions, such as illumination or lucifuge, aerobic packaging or vacuum packaging/nitrogen gas packing, normal temperature or high temperature/refrigeration/freeze, on the stability of DHA microcapsules. The results showed that light, aerobic and high temperature conditions would promote oxidization of DHA microcapsule, in which the key factor influencing the stability of DHA microcapsule is whether isolated oxygen or not. A storage test was carried out by vacuum packaging the DHA microcapsules with foil bag, stored at room temperature for eight weeks, the results showed that the POV value increased from the initial value 1.7 meq/kg to the 3.01 meq/kg, the DHA reserved rate reduced to 94.0%, and the stability of DHA microcapsules was great.%对照分析了光照与避光、有氧与真空包装/充氮包装、常温与高温/冷藏/冷冻等不同条件对DHA微胶囊的稳定性的影响.结果表明:光照、有氧条件、高温等贮藏条件均促进DHA微胶囊的氧化;其中影响DHA微胶囊稳定性的关键因索是隔绝氧气与否.将DHA微胶囊用锡箔袋真空包装,室温下放置8周,POV值仅由初始值1.7meq/kg增加到3.01 meq/kg,DHA保留率降为94.0%,DHA微胶囊的稳定性较好.

  3. PREPARATION OF EPA AND DHA ESTERS WITH HIGH PURITY BY SUPERCRITICAL CO2 EXTRACTION AND RECTIFICATION%超临界CO2萃取精馏制取高纯度EPA乙酯和DHA乙酯

    Institute of Scientific and Technical Information of China (English)

    邱榕; 范维澄; 陈钧

    2001-01-01

    Fish oil ethyl esters complexed with aqueous silver nitrate solution were extracted and rectified by supercritical CO2 to obtain DHA ester and EPA ester with high purity. The effects of some independent variables,such as extraction pressure, temperature gradient of rectifying column and programmed pressure,on rectification were investigated.The results showed that programmed pressure is suitable for purification of EPA and DHA esters. Increase of column temperature gradient from bottom to top is one of the key elements in rectification. Furthermore, higher temperature gradient leads to better separation effect.

  4. EPA和DHA对人食管鳞癌及腺癌细胞增殖的影响%The effects of EPA and DHA on proliferation of the human esophageal squamous carcinoma cells and esophageal adenocarcinoma cells

    Institute of Scientific and Technical Information of China (English)

    庄则豪; 孙静文; 曾以琳; 魏晶晶

    2013-01-01

    目的 观察EPA和DHA对食管鳞癌(ESCC)和腺癌(EAC)细胞增殖的影响,探讨可能机制.方法 EPA、DHA分别设100 μmol/L、50 μmol/L、1 μmol/L干预组及对照组.干预Eca-109细胞(ESCC)及OE-19细胞(EAC)24 h及48 h后,CCK-8法检测细胞增殖,RT-PCR及Western blot检测COX-2 mRNA和蛋白表达,ELISA法检测PGE2和PGE3水平.结果 在Eca-109细胞,高浓度EPA及DHA抑制细胞增殖,仅高浓度EPA抑制COX-2 mRNA表达;EPA及DHA呈时间-浓度依赖性抑制COX-2蛋白表达;各浓度EPA及高浓度DHA组PGE2减少、PGE3增加(P均0.05);DHA呈时间-浓度依赖性抑制COX-2 mRNA及蛋白表达,EPA则先促进后抑制其表达;EPA引起PGE2增加而PGE3减少,DHA反之(P均<0.05).结论 DHA抑制EAC细胞增殖,且与COX-2及PGE2表达抑制、PGE3增加有关.EPA对EAC细胞增殖、EPA及DHA对ESCC细胞增殖无明显影响.%Objective To investigate the effects and potential mechanisms of two major n-3 PUFAs, eicosapentaeno-ic acid (EPA) and docosahexaenoic acid (DHA) on the proliferation of ESCC and EAC cells. Methods The Eca-109 (ESCC) and OE-19 (EAC) were divided into EPA, DHA, blank control and 0. 1% ethanol solute groups. The treatment concentrations of EPA or DHA were 1 , 50 and 100 μmol/L, respectively. Cell proliferation was assessed by CCK-8 assay. Expression of COX-2 protein and mRNA were determined by Western blot and RT-PCR, respectively. The concentrations of prostaglandin E2 (PGE2) and prostaglandin E3 (PGE3) were measured by ELISA Kits. Results In Eca-109 cells, cell growth inhibition was found only in high concentration groups (100 μmol/L of EPA and DHA) ( P < 0.05). Down regulation of COX-2 protein was found in both EPA and DHA treated cells in a time- and concentration-dependent manner. Down-regulation of PGE2 and up-regulation of PGE3 expression were found in EPA treated cells and cells with high concentration DHA treatment (P <0. 05) ; In OE-19 cells, cell growth inhibition was found in DHA treated cells in a

  5. Comparison of elongatio n efficiency between DHA and EPA in syn thesis of very long chain polyunsaturated fatty acids%DHA 与 EPA 合成超长链多不饱和脂肪酸的效率比较

    Institute of Scientific and Technical Information of China (English)

    余曼; 陈波; 张瑞帆; 吴峥峥

    2014-01-01

    Objective To compare the elongation efficiency between DHA and EPA for synthesis of very long chain polyunsat -urated fatty acid (VLC-PUFAs) under catalytic action of ELOVL4 protease.Methods PC12 cells were transduced with recombinant adenovirus type 5 carrying mouse Elovl4 and green fluorescent protein (GFP).GFP-expressing and non-transduced cells were used as controls.ELOVL4 gene expression was quantified by qRT-PCRs.ELOVL4 protein was analyzed by Western-Blot (WB).The transduced cells were treated with DHA or EPA (1:1).After 48 h of incubation,cells were collected,and fatty acid methyl esters were prepared following total lipids extraction .The fatty acid was analyzed by using a gas chromatography-mass spectrometry ( GC-MS) .Results GC-MS analysis showed that the DHA and EPA treated PC 12+Ad-ELOVL4 had n3 VLC-PUFAs in which 34:5n3 and 36:5n3 were 0.85%and 1.11%,respectively;34:6n3 and 36:6n3 were 0.16% and 0.29%,respectively.Total amount of pentaenoics synthesized from EPA was almost four times than that of hexaenoics synthesized from DHA .Conclusion Elongation efficiency of VLC-PUFAs from EPA is much higher than that from DHA .Therefore,dietary supplementation of more EPA rather than DHA may provide some therapeutic benefits for patients with Stargardts'disease (STGD3).%目的:比较在ELOVL4蛋白酶催化作用下,DHA和EPA合成超长链多不饱和脂肪酸VLC-PUFA的效率。方法构建携带ELOVL4基因和绿色荧光蛋白的重组腺病毒,转入培养的 PC12细胞,通过qRT-PCR定量分析ELOVL 4基因的表达量,WB检测ELOVL4蛋白的表达;1∶1加入DHA和EPA,孵育48 h之后进行脂肪酸提取,通过气相质谱 GC-MS分析超长链脂肪酸的成分。结果 GC-MS检测到分别用DHA及EPA处理后的PC12+Ad-ELOVL4的细胞中有n3 VLC-PUFA的表达,34:5n3和36:5n3分别为0.85%和1.11%;34:6n3和36:63n分别为0.16%和0.29%;EPA所产生的五烯酸总和是DHA所产生的六烯酸总和的4

  6. Study on the optimum proportion of DHA and EPA in microdiets for red seabream (Pagrus major) larvae%真鲷仔稚鱼微粒饲料中DHA与EPA最佳比例的研究

    Institute of Scientific and Technical Information of China (English)

    刘镜恪; 陈晓琳; 周利; 雷霁霖

    2004-01-01

    制备 4种 n- 3HUFA含量相同, DHA和 EPA含量比 (m(DHA)∶ m(EPA))分别为 1.70∶ 1, 2.00∶ 1, 2.30∶ 1和 2.60∶ 1的微粒饲料,探讨 DHA与 EPA的配比对真鲷( Pagrus major)仔稚鱼生长、存活和体内相关成分的影响. 30 d的养殖试验结果表明, m(DHA)∶ m(EPA)≥ 2.30∶ 1的 2种微粒饲料与 m(DHA)∶ m(EPA)≤ 2.00∶ 1的 2种微粒饲料相比,真鲷仔稚鱼的生长速度和成活率,前者明显优于后者.该比例即 m(DHA)∶ m(EPA)≥ 2.30∶ 1应是真鲷微粒饲料中 DHA与 EPA的最佳配比.养殖试验结束后,对仔稚鱼体内相关成分的分析结果表明,仔稚鱼体内 DHA的含量随微粒饲料中 DHA含量的增加而增大.

  7. Study of a two-stage growth of DHA-producing marine algae Schizochytrium limacinum SR21 with shifting dissolved oxygen level.

    Science.gov (United States)

    Chi, Zhanyou; Liu, Yan; Frear, Craig; Chen, Shulin

    2009-01-01

    The culture protocol of Schizochytrium limacinum SR 21, a known docosahexaenoic acid (DHA) producing marine algae was modified in this study to better fit fermentation parameters, particularly control of dissolved oxygen (DO) to the known reproductive and growth biology of the microorganism. The cultures controlled at 50% DO saturation produced a cell density of 181 million cells/ml, whereas cultures with 10% DO produced only 98.4 million cells/ml. A fixed-agitation rate of 150 rpm resulted in an even lower density of 22.5 million cells/ml. Fifty percent DO saturation level led to a decreased pH, as well as a negative correlation with lipid accumulation, while low oxygen concentration was obligatory for lipid accumulation. This study indicated that high DO was preferred for the cells' reproduction via release of zoospores. Thus, the culture of S. limacinum SR21 should be best divided into two stages: (1) a cell-number-increasing stage in which cell reproduction and cell number increase with little increase in the size and weight of each cell; and (2) a cell-size-increasing stage in which cells stop reproduction but cell size enlarges due to lipids accumulation. With such a protocol, the production of algae biomass and DHA was improved to levels of 37.9 g/L and 6.56 g/L, respectively. The two-stage culture process could be potentially used not only for omega-3 PUFA production, but also in other single cell oil (SCO)-producing processes, including biodiesel production from algae.

  8. Effects of DHA-rich fish oil supplementation on the lipid profile, markers of muscle damage, and neutrophil function in wheelchair basketball athletes before and after acute exercise.

    Science.gov (United States)

    Marques, Camila Garcia; Santos, Vinicius Coneglian; Levada-Pires, Adriana Cristina; Jacintho, Thiago Manzoni; Gorjão, Renata; Pithon-Curi, Tânia Cristina; Cury-Boaventura, Maria Fernanda

    2015-06-01

    We investigated the effects of docosahexaenoic acid (DHA)-rich fish oil (FO) supplementation on the lipid profile, levels of plasma inflammatory mediators, markers of muscle damage, and neutrophil function in wheelchair basketball players before and after acute exercise. We evaluated 8 male basketball wheelchair athletes before and after acute exercise both prior to (S0) and following (S1) FO supplementation. The subjects were supplemented with 3 g of FO daily for 30 days. The following components were measured: the plasma lipid profile (total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglycerides), plasma inflammatory mediators (C-reactive protein, interleukin (IL)-1β, IL-1ra, IL-4, IL-6, IL-8, and tumor necrosis factor-α), markers of muscle damage (creatine kinase and lactate dehydrogenase (LDH)), and neutrophil function (cytokine production, phagocytic capacity, loss of membrane integrity, mitochondrial membrane potential, neutral lipid accumulation, phosphatidylserine externalization, DNA fragmentation, and production of reactive oxygen species (ROS)). Acute exercise increased the plasma levels of total cholesterol, LDH, IL1ra, and IL-6, led to the loss of membrane integrity, ROS production, and a high mitochondrial membrane potential in neutrophils, and reduced the phagocytic capacity and IL-6 production by the neutrophils (S0). However, supplementation prevented the increases in the plasma levels of LDH and IL-6, the loss of membrane integrity, and the alterations in ROS production and mitochondrial membrane potential in the neutrophils that were induced by exercise (S1). In conclusion, DHA-rich FO supplementation reduces the markers of muscle damage, inflammatory disturbances, and neutrophil death induced by acute exercise in wheelchair athletes.

  9. 河豚鱼肝油中EPA、DHA的纯化与含量测定%Purification and Content Determination of EPA and DHA in Puffer Fish Liver Oil

    Institute of Scientific and Technical Information of China (English)

    郭斌; 孟磊; 彭宏伟; 李智

    2011-01-01

    OBJECTIVE: To study purification method of EPA and DHA in the puffer fish liver oil and to establish the methods for the content determination of EPA and DHA. METHODS: Unsaturated fatty acid was purified by refinement, salt fractionation,hypothermy-sylvite method and urea clathration method. After activated by methyl ester, the content of EPA and DHA were detected by GC chromatography. RESULTS: Highly purified unsaturated fatty acids were purified from puffer fish liver oil and the content of EPA and DHA was 0.261 9 g· g-1 and 0.452 7 g· g-1, respectively. The calibration curves were linear in the ranges of 0.05-0.25 mg· mL-1 for EPA methyl ester and DHA methyl ester. CONCLUSION: It is a feasible method for purification of EPA and DHA in puffer fish liver oil. The method is simple, accurate and reproducible for the content determination.%目的:研究河豚鱼肝油中二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)的纯化方法,并建立EPA和DHA的含量测定方法.方法:河豚鱼肝油经精炼、盐析、低温-钾盐乙醇法和尿素包合法相结合纯化得不饱和脂肪酸;不饱和脂肪酸经甲脂化后,采用气相色谱法测定EPA和DHA的含量.结果:河豚鱼肝油经纯化可获得高纯度的不饱和脂肪酸;EPA和DHA的含量分别为0.261 9g·g-1和0.4527 g·g-1.EPA甲酯和DHA甲酯标准品浓度均在0.05~0.25mg·mL-1范围内同其与内标的峰面积比值呈良好的线性关系(r分别为0.999 7、0.999 8).结论:河豚鱼肝油中EPA和DHA的纯化方法可行;检测方法操作简单、结果准确、重复性良好,是一种可行的含量测定方法.

  10. 毛细管气相色谱法在血液DHA、EPA测定中的应用及评价%Application and evaluation of capillary gas chromatography for the determination of DHA and EPA in blood

    Institute of Scientific and Technical Information of China (English)

    韩光辉; 张艳丽; 贾秋桦; 公新; 刘泽伦

    2012-01-01

    Objective To evaluate the application effect of the determination of docosahexaenoic acid ( DHA) and ei-cosapentaenoic acid (EPA) in blood by capillary gas chromatographic ( CGC) method. Methods CGC method was established , and used to determinate the concentration of DHA and EPA in blood, then this method was evaluated. Results In the standard recovery test of high, middle and low levels, the DHA average recovery rate was between 92. 0 % and 93. 6% , RSD ranged from 3. 0% to 3. 6%. The EPA average recovery rate was between 92. 3% and 94. 0% , RSD ranged from 2.8% to 4.0%. Conclusion CGC method is precise, accurate, reproducible, and stable in a short time. It is suitable for the determination of DHA and EPA in blood.%目的 评价毛细管气相色谱法在血液二十二碳六烯酸(DHA)和二十碳五烯酸(EPA)检测中的应用效果.方法 建立毛细管气相色谱法,测定血液中的DHA、EPA,并对该方法进行评价.结果 高中低三个浓度的加标回收试验,DHA平均回收率为92.0% ~ 93.6%,RSD为3.0% ~3.6%,EPA的平均回收率为92.3% ~ 94.0%,RSD为2.8% ~4.0%.结论 毛细管气相色谱法精密度、准确度和重复性较好,且短时间内稳定,适用于检测血液中的DHA和EPA.

  11. Effect of DHA on Antioxidation and Fatty Acids in Aged Rat Brain%二十二碳六烯酸对老年大鼠脑组织抗氧化和脂肪酸的影响

    Institute of Scientific and Technical Information of China (English)

    蒋利和; 马博; 谢正轶; 杨志荣

    2011-01-01

    Objective:To explore the antioxidant mechanisms of DHA in the brain of aged rats.Methods:Wistar male rats were randomly divided into four groups including 7-month-old adult group,20-month-old aged group,and low-dose and high-dose DHA-treated 20-month-old aged groups at the respective doses of 180 mg/(kg·d) and 360 mg/(kg·d)(administered by gavage for 42 consecutive days).At the end of the administration period,all rats were sacrificed and the activities of superoxide dismutase(SOD),gtutathione peroxidase(GSH-Px) and catalase(CAT) and the level of malondialdehyde(MDA) were assayed in brain.In addition,the fatty acid profile in brain was analyzed by gas chromatography-mass spectrometry,and the phospholipid composition was also analyzed by thin layer chromatography and phosphomolybdate methods.Results:Compared with the aged group,the activities of SOD,GSH-Px and CAT in the brain of the DHA-treated groups were significantly increased and MDA content was significantly decreased.Fatty acid analysis showed that DHA-treated rats exhibited an obvious increase in the ratio of total n-3 fatty acids to total n-6 fatty acids and the ratio of DHA to arachidonic acid(AA) and an obvious reduction in AA level.It was also revealed that DHA could improve the levels of phosphatidylcholine,phosphatidylethanolamine,phosphatidylinositol,phosphatidylserine and sphingomgelins in the brain of rats.Conclusion:DHA can obviously improve antioxidant capacity in aged rats and its antioxidant mechanism may be due to DHA-induced change of fatty acid profile in the brain.%目的:研究二十二碳六烯酸(DHA)改善老年大鼠抗氧化的可能机制。方法:动物分为青年组(7月龄青年雄性大鼠)、老年组(20月龄雄性大鼠)、DHA低剂量组(灌喂20月龄雄性大鼠DHA 180mg/(kg.d)42d)、DHA高剂量组(灌喂20月龄雄性大鼠360mg/(kg.d)42d),处死大鼠后测定脑组织中超氧化物歧化酶

  12. Dietary fatty acid composition is sensed by the NLRP3 inflammasome: omega-3 fatty acid (DHA) prevents NLRP3 activation in human macrophages.

    Science.gov (United States)

    Martínez-Micaelo, N; González-Abuín, N; Pinent, M; Ardévol, A; Blay, M

    2016-08-10

    The Nod-like receptor protein 3 (NLRP3) inflammasome is considered to be a pivotal host platform responsible for sensing of exogenous and endogenous danger signals, including those generated as a result of metabolic dysregulation, and for the subsequent, IL-1β-mediated orchestration of inflammatory and innate immunity responses. In this way, although the molecular link between diet-induced obesity and inflammasome activation is still unclear, free fatty acids (FFA) have been proposed as a triggering event. We report that dietary fatty acid (FA) composition is sensed by the NLRP3 inflammasome in human macrophages. For this purpose, we have analysed three roles of FA supplementation: as a priming signal for ATP-activated macrophages, in determining where the administration of dietary FAs interferes with LPS-mediated inflammasome activation and by inducing inflammasome activation per se. In this study, we confirm that saturated (SFAs) activated the NLRP3 inflammasome and stimulated the secretion of the IL-1β cytokine, while PUFAs were mainly inhibitors. Moreover, in general, DHA (n-3 PUFA) was more effective in preventing inflammasome activation than arachidonic acid (n-6 PUFA). PMID:27405925

  13. 高纯度EPA/DHA甘油三酯的酶法合成%Enzymatic Synthesis of High Purity Triglyceride of EPA and DHA

    Institute of Scientific and Technical Information of China (English)

    陈小娥; 方旭波; 陈洁; 全文琴; 余辉

    2009-01-01

    利用脂肪酶分别催化游离型EPA和DHA与甘油发生酯化反应生成甘油三酯,考察了合成的影响因素.结果表明,正己烷6 mL,甘油/EPA(DHA)摩尔比为l:3(以0.4 mmol甘油为基准),Novozym 435添加量为100 mg,反应温度40℃,振荡频率150 r/min,反应24 h后添加1 g分子筛,反应时间48 h,EPA甘油三酯与DHA甘油三酯的得率分别可达88.64%和88.07%,EPA与DHA酯化度分别可达95.0%和94.5%.分析结果表明,所得产物为EPA甘油三酯和DHA甘油三酯.

  14. In Vitro Activity of Imipenem and Colistin against a Carbapenem-Resistant Klebsiella pneumoniae Isolate Coproducing SHV-31, CMY-2, and DHA-1.

    Science.gov (United States)

    Tang, Hung-Jen; Ku, Yee-Huang; Lee, Mei-Feng; Chuang, Yin-Ching; Yu, Wen-Liang

    2015-01-01

    We investigated the synergism of colistin and imipenem against a multidrug-resistant K. pneumoniae isolate which was recovered from a severe hip infection. PCR and DNA sequencing were used to characterize the outer membrane porin genes and the resistance genes mediating the common β-lactamases and carbapenemases. Synergism was evaluated by time-kill studies. The bla SHV-31, bla CMY-2, and bla DHA-1 were detected. Outer membrane porin genes analysis revealed loss of ompK36 and frame-shift mutation of ompK35. The common carbapenemase genes were not found. Time-kill studies demonstrated that a combination of 1x MIC of colistin (2 mg/L) and 1x MIC of imipenem (8 mg/L) was synergistic and bactericidal but with inoculum effect. Bactericidal activity without inoculum effect was observed by concentration of 2x MIC of colistin alone or plus 2x MIC of imipenem. In conclusion, colistin plus imipenem could be an alternative option to treat carbapenem-resistant K. pneumoniae infections. PMID:26064923

  15. In Vitro Activity of Imipenem and Colistin against a Carbapenem-Resistant Klebsiella pneumoniae Isolate Coproducing SHV-31, CMY-2, and DHA-1

    Directory of Open Access Journals (Sweden)

    Hung-Jen Tang

    2015-01-01

    Full Text Available We investigated the synergism of colistin and imipenem against a multidrug-resistant K. pneumoniae isolate which was recovered from a severe hip infection. PCR and DNA sequencing were used to characterize the outer membrane porin genes and the resistance genes mediating the common β-lactamases and carbapenemases. Synergism was evaluated by time-kill studies. The blaSHV-31, blaCMY-2, and blaDHA-1 were detected. Outer membrane porin genes analysis revealed loss of ompK36 and frame-shift mutation of ompK35. The common carbapenemase genes were not found. Time-kill studies demonstrated that a combination of 1x MIC of colistin (2 mg/L and 1x MIC of imipenem (8 mg/L was synergistic and bactericidal but with inoculum effect. Bactericidal activity without inoculum effect was observed by concentration of 2x MIC of colistin alone or plus 2x MIC of imipenem. In conclusion, colistin plus imipenem could be an alternative option to treat carbapenem-resistant K. pneumoniae infections.

  16. Effects of stripping steam on the quality of microalgae DHA oil in the deodorization process%汽提蒸汽量在脱臭工艺中对微藻DHA油脂品质的影响

    Institute of Scientific and Technical Information of China (English)

    蒋露; 胡耀池; 梁井瑞; 龚东平; 陈园力; 张红漫

    2011-01-01

    The bleached microalgae DH A oil was deodorized in a self - designed deodorization equipment. Under the deodorization conditions of temperature of 180t ,time of 5 h and pressure of -0. 1 MPa,the effects of different amount of steam on the acid value,peroxide value,soap content,unsaponifiable matter, DHA content, sterol content and color of microalgae DHA oil were investigated by adjusting the amount of steam in deodorization process. The result indicated that the peroxide value,acid value,soap content,unsaponifiable matter, sterol content and the color of the microalgae DHA oil decreased with the increase of the amount of stripping steam,meanwhile,the oil loss increased. During the processing,the DHA content exhibited no significant difference.%在自行设计的脱臭装置上对微藻DHA脱色油进行脱臭处理.在脱臭温度180℃、脱臭时间5h、脱臭压力-0.1 MPa的条件下,通过调节脱臭过程中的蒸汽量,考察了不同蒸汽量对微藻DHA油脂酸值、过氧化值、含皂量、不皂化物、DHA含量、甾醇含量以及色泽的影响.结果表明:随着汽提蒸汽量的增加,DHA油脂的过氧化值、酸值、含皂量、不皂化物、甾醇含量以及色泽均随之降低,DHA油脂的损耗随之增加,但不同的汽提蒸汽量对DHA含量影响却不大.

  17. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the extension of use for DHA and EPA-rich algal oil from Schizochytrium sp. as a Novel Food ingredient

    OpenAIRE

    Tetens, Inge

    2014-01-01

    Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on an extension of use for docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)-rich algal oil from Schizochytrium sp. as a novel food ingredient (NFI) in the context of Regulation (EC) No 258/97. The NFI is already authorised for use in a range of foodstuffs at specified maximum levels. The applicant requests an extension of use ...

  18. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion related to the Tolerable Upper Intake Level of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA)

    OpenAIRE

    Tetens, Inge

    2012-01-01

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to deliver a scientific opinion on the Tolerable Upper Intake Level (UL) of the n-3 LCPUFAs eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA). Available data are insufficient to establish a UL for n-3 LCPUFA (individually or combined) for any population group. At observed intake levels, consumption of n-3 LCPUFA has not been associated with ...

  19. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to DHA and contribution to normal brain development pursuant to Article 14 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following an application from DSM Nutritional Products, submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of the United Kingdom, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver...... an opinion on the scientific substantiation of a health claim related to docosahexaenoic acid (DHA) and contribution to normal brain development. The Panel considers that DHA is sufficiently characterised, and that contribution to normal brain development is a beneficial physiological effect for infants...... and contribution to normal brain development. The following wording reflects the scientific evidence: “DHA contributes to normal brain development”. In order to bear the claim, foods for older infants and young children below the age of 24 months should provide a daily intake of 100 mg DHA in one or more servings...

  20. Rise in DPA Following SDA-Rich Dietary Echium Oil Less Effective in Affording Anti-Arrhythmic Actions Compared to High DHA Levels Achieved with Fish Oil in Sprague-Dawley Rats

    Directory of Open Access Journals (Sweden)

    Mahinda Y. Abeywardena

    2016-01-01

    Full Text Available Stearidonic acid (SDA; C18:4n-3 has been suggested as an alternative to fish oil (FO for delivering health benefits of C ≥ 20 long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA. Echium oil (EO represents a non-genetically-modified source of SDA available commercially. This study compared EO and FO in relation to alterations in plasma and tissue fatty acids, and for their ability to afford protection against ischemia-induced cardiac arrhythmia and ventricular fibrillation (VF. Rats were fed (12 weeks diets supplemented with either EO or FO at three dose levels (1, 3 and 5% w/w; n = 18 per group. EO failed to influence C22:6n-3 (DHA but increased C22:5n-3 (DPA in tissues dose-dependently, especially in heart tissue. Conversely, DHA in hearts of FO rats showed dose-related elevation; 14.8%–24.1% of total fatty acids. Kidney showed resistance for incorporation of LC n-3 PUFA. Overall, FO provided greater cardioprotection than EO. At the highest dose level, FO rats displayed lower (p < 0.05 episodes of VF% (29% vs. 73% and duration (22.7 ± 12.0 vs. 75.8 ± 17.1 s than the EO group but at 3% EO was comparable to FO. We conclude that there is no endogenous conversion of SDA to DHA, and that DPA may be associated with limited cardiac benefit.

  1. Preliminary Validation of a High Docosahexaenoic Acid (DHA) and -Linolenic Acid (ALA) Dietary Oil Blend: Tissue Fatty Acid Composition and Liver Proteome Response in Atlantic Salmon (Salmo salar) Smolts

    Science.gov (United States)

    Nuez-Ortín, Waldo G.; Carter, Chris G.; Wilson, Richard; Cooke, Ira; Nichols, Peter D.

    2016-01-01

    Marine oils are important to human nutrition as the major source of docosahexaenoic acid (DHA), a key omega-3 long-chain (≥C20) polyunsaturated fatty acid (n-3 LC-PUFA) that is low or lacking in terrestrial plant or animal oils. The inclusion of fish oil as main source of n-3 LC-PUFA in aquafeeds is mostly limited by the increasing price and decreasing availability. Fish oil replacement with cheaper terrestrial plant and animal oils has considerably reduced the content of n-3 LC-PUFA in flesh of farmed Atlantic salmon. Novel DHA-enriched oils with high alpha-linolenic acid (ALA) content will be available from transgenic oilseeds plants in the near future as an alternative for dietary fish oil replacement in aquafeeds. As a preliminary validation, we formulated an oil blend (TOFX) with high DHA and ALA content using tuna oil (TO) high in DHA and the flaxseed oil (FX) high in ALA, and assessed its ability to achieve fish oil-like n-3 LC-PUFA tissue composition in Atlantic salmon smolts. We applied proteomics as an exploratory approach to understand the effects of nutritional changes on the fish liver. Comparisons were made between fish fed a fish oil-based diet (FO) and a commercial-like oil blend diet (fish oil + poultry oil, FOPO) over 89 days. Growth and feed efficiency ratio were lower on the TOFX diet. Fish muscle concentration of n-3 LC-PUFA was significantly higher for TOFX than for FOPO fish, but not higher than for FO fish, while retention efficiency of n-3 LC-PUFA was promoted by TOFX relative to FO. Proteomics analysis revealed an oxidative stress response indicative of the main adaptive physiological mechanism in TOFX fish. While specific dietary fatty acid concentrations and balances and antioxidant supplementation may need further attention, the use of an oil with a high content of DHA and ALA can enhance tissue deposition of n-3 LC-PUFA in relation to a commercially used oil blend. PMID:27556399

  2. Preliminary Validation of a High Docosahexaenoic Acid (DHA) and -Linolenic Acid (ALA) Dietary Oil Blend: Tissue Fatty Acid Composition and Liver Proteome Response in Atlantic Salmon (Salmo salar) Smolts.

    Science.gov (United States)

    Nuez-Ortín, Waldo G; Carter, Chris G; Wilson, Richard; Cooke, Ira; Nichols, Peter D

    2016-01-01

    Marine oils are important to human nutrition as the major source of docosahexaenoic acid (DHA), a key omega-3 long-chain (≥C20) polyunsaturated fatty acid (n-3 LC-PUFA) that is low or lacking in terrestrial plant or animal oils. The inclusion of fish oil as main source of n-3 LC-PUFA in aquafeeds is mostly limited by the increasing price and decreasing availability. Fish oil replacement with cheaper terrestrial plant and animal oils has considerably reduced the content of n-3 LC-PUFA in flesh of farmed Atlantic salmon. Novel DHA-enriched oils with high alpha-linolenic acid (ALA) content will be available from transgenic oilseeds plants in the near future as an alternative for dietary fish oil replacement in aquafeeds. As a preliminary validation, we formulated an oil blend (TOFX) with high DHA and ALA content using tuna oil (TO) high in DHA and the flaxseed oil (FX) high in ALA, and assessed its ability to achieve fish oil-like n-3 LC-PUFA tissue composition in Atlantic salmon smolts. We applied proteomics as an exploratory approach to understand the effects of nutritional changes on the fish liver. Comparisons were made between fish fed a fish oil-based diet (FO) and a commercial-like oil blend diet (fish oil + poultry oil, FOPO) over 89 days. Growth and feed efficiency ratio were lower on the TOFX diet. Fish muscle concentration of n-3 LC-PUFA was significantly higher for TOFX than for FOPO fish, but not higher than for FO fish, while retention efficiency of n-3 LC-PUFA was promoted by TOFX relative to FO. Proteomics analysis revealed an oxidative stress response indicative of the main adaptive physiological mechanism in TOFX fish. While specific dietary fatty acid concentrations and balances and antioxidant supplementation may need further attention, the use of an oil with a high content of DHA and ALA can enhance tissue deposition of n-3 LC-PUFA in relation to a commercially used oil blend. PMID:27556399

  3. Pathways of Lipid Metabolism in Marine Algae, Co-Expression Network, Bottlenecks and Candidate Genes for Enhanced Production of EPA and DHA in Species of Chromista

    Directory of Open Access Journals (Sweden)

    Alice Mühlroth

    2013-11-01

    Full Text Available The importance of n-3 long chain polyunsaturated fatty acids (LC-PUFAs for human health has received more focus the last decades, and the global consumption of n-3 LC-PUFA has increased. Seafood, the natural n-3 LC-PUFA source, is harvested beyond a sustainable capacity, and it is therefore imperative to develop alternative n-3 LC-PUFA sources for both eicosapentaenoic acid (EPA, 20:5n-3 and docosahexaenoic acid (DHA, 22:6n-3. Genera of algae such as Nannochloropsis, Schizochytrium, Isochrysis and Phaedactylum within the kingdom Chromista have received attention due to their ability to produce n-3 LC-PUFAs. Knowledge of LC-PUFA synthesis and its regulation in algae at the molecular level is fragmentary and represents a bottleneck for attempts to enhance the n-3 LC-PUFA levels for industrial production. In the present review, Phaeodactylum tricornutum has been used to exemplify the synthesis and compartmentalization of n-3 LC-PUFAs. Based on recent transcriptome data a co-expression network of 106 genes involved in lipid metabolism has been created. Together with recent molecular biological and metabolic studies, a model pathway for n-3 LC-PUFA synthesis in P. tricornutum has been proposed, and is compared to industrialized species of Chromista. Limitations of the n-3 LC-PUFA synthesis by enzymes such as thioesterases, elongases, acyl-CoA synthetases and acyltransferases are discussed and metabolic bottlenecks are hypothesized such as the supply of the acetyl-CoA and NADPH. A future industrialization will depend on optimization of chemical compositions and increased biomass production, which can be achieved by exploitation of the physiological potential, by selective breeding and by genetic engineering.

  4. DHA提高大鼠学习记忆的分子机制研究%Molecular Mechanism of DHA for Improving Learning and Memory in Rats

    Institute of Scientific and Technical Information of China (English)

    马琳; 王丽梅; 樊永波; 吴晨; 陶兴无; 刘志国; 刘烈炬

    2013-01-01

    目的:研究二十二碳六烯酸(DHA)对大鼠学习记忆的影响并探讨其作用的分子机制.方法:将32只初断乳SD雄性大鼠,按随机数字表法分成4组:阴性对照组、阳性对照组、DHA低剂量组(370mg/(kg·d))和DHA高剂量组(740mg/(kg·d)).连续饲养8周,测定大鼠脑组织中乙酰胆碱酯酶(AChE)、一氧化氮合酶(NOS)的活性,其次,采用RT-PCR方法测定大鼠脑部海马组织中N-甲基-D-天冬氨酸(NMDA)受体亚基(NR1)、cAMP反应元件结合蛋白(CERB)、即刻早期基因(c-fos)mRNA的表达水平.结果:与阴性对照组相比,DHA高、低剂量均能提高大鼠脑组织AChE和NOS的活性,并且能够显著上调海马组织内NR1、CRE、c-fos基因mRNA的表达(P<0.01),但以低剂量为佳.结论:DHA能够提高大鼠学习记忆能力并且存在一定的量效关系,其机制与上调海马组织内NR1、CREB、c-fos基因mRNA的表达水平有关.

  5. [Therapeutic effects of larger doses of arachidonic acid added to DHA on social impairment and its relation to alterations of polyunsaturated fatty acids in individuals with autism spectrum disorders].

    Science.gov (United States)

    Yui, Kunio; Koshiba, Mamiko; Nakamura, Shun; Onishi, Masako

    2011-06-01

    The polyunsaturated fatty acids (PUFAs), arachidonic acid (ARA) and docosahexaenoic acid (DHA) may play key roles in brain network maturation. ARA plays an important role in signal transduction related to neuronal maturation. This study aims to evaluate the efficacy of supplementing with larger doses of ARA added to DHA in a double-blind, placebo-controlled 16-week trial. To confirm findings observed in the placebo-controlled trial, an additional 16-week open-label study was further conducted. To examine the relationship between the efficacy of the supplementation regimen and alterations in PUFAs levels, we examined plasma levels of PUFAs. We used the Social Responsiveness Scale (SRS) and the Aberrant Behavior Checklist-Community (ABC) to estimate psychotic symptoms. Repeated measures ANOVA revealed that this supplementation significantly improved SRS-measured communication as well as ABC-measured social withdrawal during the placebo-controlled trial. The treatment effect sizes were more favorable for the treatment group compared with the placebo group (communication: 0.87 vs. 0.44; social withdrawal: 0.88 vs. 0.54). At the end of the placebo-controlled trial, there was a significant difference in the change in plasma ARA levels from the baseline and a trend towards a significant difference in plasma ARA levels between the two groups. The open-label study was not powered to detect significant improvements in the outcome measures or significant differences in plasma ARA levels. The present clinical trials suggest that supplementation with larger ARA doses added to DHA improves social impairment in individuals with ASD via ARA-induced upregulation of neuronal functioning. PMID:21800702

  6. Lipidomics and H218O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio) embryos

    OpenAIRE

    McDougall, Melissa Q.; Jaewoo Choi; Stevens, Jan F.; Lisa Truong; Tanguay, Robert L.; Traber, Maret G.

    2016-01-01

    We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without (E−) or with added α-tocopherol (E+, 500 mg RRR-α-tocopheryl acetate/kg diet) for a minimum of 80 days, and then spawned them to obta...

  7. THE EFFECTS OF DIFFERENT LEVELS OF EPA AND DHA ON LIPIDS METABOLISM, GROWTH AND REPRODUCTIVE PERFORMANCE OF RICE FIELD EEL(MONOPTERUS ALBUS)%饲料中不同EPA和DHA含量对黄鳝脂类代谢、生长及繁殖性能的影响

    Institute of Scientific and Technical Information of China (English)

    朱长生; 江波; 周秋白

    2013-01-01

    为研究 EPA 和 DHA 对黄鳝脂类代谢、生长及繁殖性能的影响,用添加有 EPA 和 DHA 比例相同(EPA/DHA=0.35)但水平不同(EPA和DHA总量分别为0.00%、0.35%、0.70%)的3种等氮等脂肪饲料,在常温(22-30℃)下,分别喂养平均体重为(21.71±0.54) g的黄鳝7周,分析黄鳝脂类代谢和生长性能,然后每组取15尾继续养殖至性腺发育成熟用于繁殖性能分析。结果表明:在饲料中添加0.70%的EPA和DHA 能促进黄鳝生长,显著提高黄鳝的特定生长率(SGR)及肌肉粗蛋白含量,降低肝体指数(HSI)及肌肉、肝脏中的粗脂肪含量(P<0.05),显著提高亲鳝的性体指数(GSI)、产卵量、孵化率、幼苗的存活指数(SAI)(P<0.05)。添加0.35%的EPA和DHA能显著降低肝脏中粗脂肪含量(P<0.05)。综合分析表明: EPA和DHA能促进黄鳝的脂类代谢,显著提高其生长及繁殖性能;饲料中添加0.70%的EPA和DHA较为适宜。%A feeding experiment was conducted to investigate the influences of EPA and DHA of dietary lipids on lipids metabolism, growth and reproductive performance of rice field eel (Monopterus albus Zuiew). Fishes of which the av-erage weight was (21.71±0.54) g were fed with three isonitrogenous and isolipidic experimental diets with the same EPA/DHA ratios (EPA∶DHA=0.35) but different amount of EPA and DHA (0.00%, 0.35%and 0.70%, respectively) for seven weeks at room temperature ranging from 22℃ to 30℃, then the lipid metabolism and growth performance were analyzed. After that fifteen fishes from each group were took to keep until gonadal maturation for reproductive performance analysis. Results showed that the diet containing 0.70%of EPA and DHA could promote the growth of rice field eels and significantly increased the specific growth rate (SGR) and the protein content of muscle, but decreased the hepatoso-matic index (HSI) and the fat content of liver and muscle (P<0.05). Significant increments of spawning

  8. Clonal spread of both oxyimino-cephalosporin- and cefoxitin-resistant Klebsiella pneumoniae isolates co-producing SHV-2a and DHA-1 beta-lactamase at a burns intensive care unit.

    Science.gov (United States)

    Song, Wonkeun; Lee, Kyu Man; Kim, Han-Sung; Kim, Jae-Seok; Kim, Jonghyun; Jeong, Seok Hoon; Roh, Kyoung Ho

    2006-12-01

    Over a 1-month period, a total of 16 ceftriaxone- and cefoxitin-resistant Klebsiella pneumoniae isolates were isolated from 15 patients hospitalised at a burns intensive care unit (ICU). These isolates showed negative results for extended-spectrum beta-lactamase (ESBL) by the Vitek system and were highly resistant to ceftazidime, aztreonam and cefoxitin (minimum inhibitory concentrations > or =128 microg/mL). The bla(SHV-2a) and bla(DHA-1) genes were detected by polymerase chain reaction and sequence analysis. Pulsed-field gel electrophoresis profiles of the isolates were identical. AmpC disk tests for AmpC enzymes as well as double-disk tests and Clinical and Laboratory Standards Institute (CLSI) confirmatory disk tests for ESBLs yielded positive results for all the isolates. However, only three isolates (18.8%) were shown to produce ESBL by CLSI confirmatory tests using broth microdilution. We report the first outbreak of colonisations and infections due to K. pneumoniae isolates co-producing an SHV-2a ESBL and a DHA-1 AmpC beta-lactamase in a Korean hospital, which were suggested to represent a single clonal spread at a burns ICU. In addition, this report presents problems associated with ESBL detection using broth microdilution in isolates that co-produce an ESBL and an AmpC beta-lactamase. PMID:17095195

  9. Backbone and sidechain 1H, 13C and 15N resonance assignments of the human brain-type fatty acid binding protein (FABP7) in its apo form and the holo forms binding to DHA, oleic acid, linoleic acid and elaidic acid

    DEFF Research Database (Denmark)

    Oeemig, Jesper S; Jørgensen, Mathilde L; Hansen, Mikka S;

    2009-01-01

    In this manuscript, we present the backbone and side chain assignments of human brain-type fatty acid binding protein, also known as FABP7, in its apo form and in four different holo forms, bound to DHA, oleic acid, linoleic acid and elaidic acid.......In this manuscript, we present the backbone and side chain assignments of human brain-type fatty acid binding protein, also known as FABP7, in its apo form and in four different holo forms, bound to DHA, oleic acid, linoleic acid and elaidic acid....

  10. Optimization of fermentation process for production of DHA oil by Schizochytrium sp.%裂殖壶菌发酵产DHA油脂的生产工艺优化

    Institute of Scientific and Technical Information of China (English)

    黎丽; 窦光鹏; 霍文严; 魏巍

    2015-01-01

    采用磷酸香草醛法实时监测裂殖壶菌发酵产DHA油脂的积累情况,对裂殖壶菌的基础发酵工艺进行了优化。得到裂殖壶菌生长和油脂积累的最佳培养基配方为:葡萄糖30 g/L,玉米浆粉6 g/L,蛋白胨4 g/L,硝酸钠3.6~3.9 g/L,海水晶15 g/L;在50 L的发酵罐中采用后期流加一定量的葡萄糖提高碳氮比来提高油脂积累外,通过流加3.0 g/L的大豆油来刺激菌体生长,最终经过72 h的流加培养,菌体湿重达到200 g/L,总油脂含量达到60%以上,油脂脂肪酸组成中的DHA含量占22%左右。%The accumulation of DHA oil produced by Schizochytrium sp. was real-timely monitored by phosphoric acid vanillin method, and the fermentation process for production of DHA oil by Schizochytri-um sp. was optimized. The results showed that the optimal formular of culture medium for growth and oil accumulation of Schizochytrium sp. were obtained as follows:glucose 30 g/L, corn steep powder 6 g/L, peptone 4 g/L, sodium nitrate 3. 6-3. 9 g/L, sea crystal 15 g/L. A certain dosage of glucose was fed to increase carbon-nitrogen ratio to improve oil accumulation and a fed batch of 3 . 0 g/L soybean oil could stimulate cell growth in 50 L fermentor. After fed batch fermentation for 72 h, wet weight of cell reached 200 g/L,total oil content was more than 60% and DHA content in the fatty acid composition of the oil ac-counted for about 22%.

  11. Determination of DHA and EPA in fish oil lipid emulsion injection by GC%毛细管气相色谱法测定鱼油脂肪乳注射液中DHA和EPA的含量

    Institute of Scientific and Technical Information of China (English)

    靖会; 赵惠茹; 扈本荃

    2011-01-01

    The content of DHA and EPA in fish oil lipid emulsion injection was determined by capillary gas chromatographic method with directly sampling. The chromatographic column was PEC-20M. The column temperature was programmed. The carrier gas was helium and the flow rate was 0. 5 mL/min. The detector was FID and its temperature was 280 ℃. The injection temperature was 300 ℃ , the split ratio was 5 ∶ 1 , and the injection volume was 1 μL. Results showed that the linear rang of DHA ester and EPA ester was 0. 197 ~0. 690 g/L and 0. 208 ~0. 743 g/L,r was 0. 999 5 and 0. 998 4, respectively. The average recovery was 99 . 99% and 99 . 4l% , RSD was 0. 52% and 0. 47 % , respectively. The method was simple ,accurate and reproducible and it can be used to determinate the concentration of DHA and EPA.%采用直接进样毛细管气相色谱法测定鱼油脂肪乳注射液中DHA和EPA的含量,色谱柱为PEG-20M毛细管柱,柱温采用程序升温,载气为氦气,流速0.5 mL/min,采用FID检测器,温度为280℃,进样口温度300 ℃,分流比5∶1,进样量1 μL.结果表明,DHA甲酯及EPA甲酯浓度分别为0.197~0.690 g/L,0.208~0.743 g/L时线性关系良好,r分别为0.999 5,0.998 4;平均l旦I收率分别为99.99%,99.41%,RSD分别为0.52%,0.47%.该法简便、准确,重现性好,可用于鱼油脂肪乳注射液中DHA和EPA的含量测定.

  12. Modificación de la transferencia de nutrientes en la cadena nutritiva microalga-rotífero y microalga-Artemia mediante cambios en la formulación de los nutrientes en el cultivo microalgal

    OpenAIRE

    Ronsón Paulín, José Ángel

    2011-01-01

    Las densidades celulares de los cultivos discontinuo y semicontinuo en Phaeodactylum tricurnutum e Isochrysis galbana var. T-iso, utilizando las diferentes concentraciones de Ca2+, Mg2+, Se2-, Si4+, Fe3+, Zn2+, Cu2+, y S2- produjo diferencias altamente significativas así mismo, en la composición bioquímica y en el conjunto de los ácidos grasos ω-3 y con énfasis en los ácidos grasos eicosapentaenoico (EPA) y docosahexaenoico (DHA) respectivamente. Estos resultados sugirieron que determinadas c...

  13. Growth and the content of EPA and DHA in three algae under two culture methods%3种微藻兼养培养及营养成分的比较

    Institute of Scientific and Technical Information of China (English)

    孙灵毅; 王力勇; 赵强

    2004-01-01

    对三角褐指藻Phaeodactylum tricornutum、亚心形扁藻Platymonas subcordiformis、等鞭金藻Isochrysis galbana 3011品系分别进行自养培养和兼养培养试验,结果表明:三角褐指藻、等鞭金藻在兼养培养条件下,其生长速度都不同程度地增加,EPA和DHA含量也显著地提高;而亚心形扁藻在兼养培养条件下却表现出很大的差别,生长速度没有改变,EPA的含量极低,并且不含DHA.

  14. Biomass, Total Lipids and Fatty Acid EPA/DHA Composition of 11 Species of Microalgae%11种微藻脂类和EPA/DHA组成的研究

    Institute of Scientific and Technical Information of China (English)

    林学政; 李光友

    2000-01-01

    研究了11种微藻在可比较的条件下,其生物量、总脂含量和多不饱和脂肪酸(EPA/DHA)的组成.结果表明:卡德藻(Tetraselmissp.)的生物量最大,细胞干重可达0.140g/(L·d);南极冰藻(Berkeleyarutdans)次之,可达0.0748/(L·d).以叉鞭金藻(Dicrateriainornata)和球等鞭金藻(Isochrysisgalbana)的总酯含量最高,分别占细胞干重的13.1%和12.1%.在绿色巴夫藻(Pavlovaviridis)中,EPA和DHA分别占总脂肪酸(TFA)的25%和6%;小球藻(Chlorellasp.)的EPA含量为28%;南极冰藻的EPA含量为19%.

  15. First characterisation of plasmid-mediated quinolone resistance-qnrS1 co-expressed bla CTX-M-15 and bla DHA-1 genes in clinical strain of Morganella morganii recovered from a Tunisian Intensive Care Unit

    Directory of Open Access Journals (Sweden)

    S Mahrouki

    2012-01-01

    Full Text Available Purpose: Aim of this study was to show the emergence of the qnr genes among fluoroquinolone-resistant, AMPC and ESBL (extended-spectrum-beta-lactamase co-producing Morganella morganii isolate. Materials and Methods: A multi resistant Morganella morganii SM12012 isolate was recovered from pus from a patient hospitalized in the intensive care unit at the Military hospital, Tunisia. Antibiotic susceptibility was tested with the agar disk diffusion method according to Clinical and Laboratory Standards Institute guidelines. ESBLs were detected using a standard double-disk synergy test. The characterization of beta-lactamases and associated resistance genes were performed by isoelectric focusing, polymerase chain reaction and nucleotide sequencing. Results: The antimicrobial susceptibility testing showed the high resistance to penicillins, cephalosporins (MICs: 64-512 μg/ml and fluoroquinolones (MICs: 32-512 μg/ml. But M. morganii SM12012 isolate remained susceptible to carbapenems (MICs: 4-<0.25 μg/ml. The double-disk synergy test confirmed the phenotype of extended-spectrum β-lactamases (ESBLs. Three identical β-lactamases with pI values of 6.5, 7.8 and superior to 8.6 were detected after isoelectric focusing analysis. These β-lactamases genes can be successfully transferred by the conjugative plasmid. Molecular analysis demonstrated the co-production of bla DHA-1, bla CTX-M-15 and qnrS1 genes on the same plasmid. The detection of an associated chromosomal quinolone resistance revealed the presence of a parC mutation at codon 80 (Ser80-lle80. Conclusion: This is the first report in Tunisia of nosocomial infection due to the production of CTX-M-15 and DHA-1 β-lactamases in M. morganii isolate with the association of quinolone plasmid resistance. The incidence of these strains invites continuous monitoring of such multidrug-resistant strains and the further study of their epidemiologic evolution.

  16. アルテミア摂餌期におけるノコギリガザミ幼生への EPA と DHA の給餌適正量

    OpenAIRE

    小林, 孝幸; 竹内, 俊郎; 荒井, 大介; 関谷, 幸生; KOBAYASHI, TAKAYUKI; Takeuchi, Toshio; Arai, Daisuke; Sekiya, Sachio

    2000-01-01

    アルテミア摂餌期におけるノコギリガザミ幼生へのエイコサペンタエン酸(EPA)とドコサヘキサエン酸(DHA)の給餌適正量を明らかにすることを目的に, 各齢期の生残率, 平均到達日数, 第1齢稚ガニの全甲幅長および餌料中の脂肪酸含量等を検討した。その結果, ノコギリガザミ幼生は生残に対してEPAを強く要求し, 乾燥重量当り1.3∿2.5%程度必要であるものと推察された。また, DHAは第1齢稚ガニの全甲幅長を大きくすることに機能するが, 0.46%以上強化するとメガロパ幼生への変態に際し, 脱皮失敗によるへい死率の増加がみられることが明らかとなった。The suitable dietary levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were determined for larval mud crab during the Artemia feeding period. Two feeding experiments were carried out in one liter pl...

  17. Isolation of a novel C18-Δ9 polyunsaturated fatty acid specific elongase gene from DHA-producing Isochrysis galbana H29 and its use for the reconstitution of the alternative Δ8 pathway in Saccharomyces cerevisiae.

    Science.gov (United States)

    Li, Ming; Ou, Xiuyuan; Yang, Xiangdong; Guo, Dongquan; Qian, Xueyan; Xing, Laijun; Li, Mingchun

    2011-09-01

    A novel gene (IgASE2) encoding a C18-Δ9 polyunsaturated fatty acids specific (C18-Δ9-PUFAs-specific) elongase was isolated and characterized from DHA-rich microalga, Isochrysis galbana H29. The IgASE2 gene was 1,653 bp in length, contained a 786 bp ORF encoding a protein of 261 amino acids that shared 87% identity with Δ9 elongase, IgASE1, and possessed a 44 bp 5'-untranslated region (5'-UTR) and a 823 bp 3'-untranslated region (3'-UTR). IgASE2, by its heterologous expression in Saccharomyces cerevisiae, elongated linoleic acid (LA, 18:2n-6) and α-linolenic (ALA, 18:3n-3) to eicosadienoic acid (EDA, 20:2n-6) and eicosatrienoic acid (ETrA, 20:3n-3), respectively. The conversions of LA to EDA and ALA to ETrA were 57.6 and 56.1%, respectively. Co-expression of this elongase with Δ8 desaturase required for the synthesis of C20-polyunsaturated fatty acids resulted in the accumulation of dihomo-γ-linolenic acid (20:3n-6) from LA and eicosatetraenoic acid (20:4n-6) from ALA. These results demonstrated that IgASE2 exhibited C18-Δ9-PUFAs-specific elongase activity and the alternative Δ8 pathway was reconstituted.

  18. Production of a Single Cell Oil Which is Highly Enriched in DHA by Marine Microalgae%利用海洋微藻生产富含DHA的单细胞油脂

    Institute of Scientific and Technical Information of China (English)

    潘冰蜂; 李祖义

    2000-01-01

    DHA是神经组织的基本组分,人体合成DHA的能力有限,从饮食中摄取足够的DHA十分重要.特别是婴幼儿缺乏DHA会影响智力、视力和生理发育.鱼油是目前DHA的最普遍来源,但是鱼油的产量和质量难以满足人们的需要.利用生物技术生产DHA是发展方向,研究得最多的是用海洋真菌和藻类生产DHA,其中用海洋藻类Crhpthecodinium cohnii生产富含DHA的单细胞油脂已达工业化规模,而且产品的稳定性,纯度,安全性和生物利用率都优于鱼油,目前已应用于婴儿奶粉和作为辅助食品.

  19. No effect of 12 weeks' supplementation with 1 g DHA-rich or EPA-rich fish oil on cognitive function or mood in healthy young adults aged 18-35 years.

    Science.gov (United States)

    Jackson, Philippa A; Deary, Michael E; Reay, Jonathon L; Scholey, Andrew B; Kennedy, David O

    2012-04-01

    The n-3 PUFA are a unique class of fatty acids that cannot be manufactured by the body, and must be acquired via dietary sources. In the UK, as well as in other Western nations, these 'essential' fatty acids are consumed in quantities that fall below government guidelines. The present study explored the effects of 12 weeks' dietary supplementation with 1 g/d of two types of fish oil (FO; DHA-rich and EPA-rich) in 159 healthy young adults aged 18-35 years. An assessment of performance on a battery of computerised cognitive tasks and mood measures took place before and following the 12-week treatment regimen. Venous blood samples were also supplied by participants at both time points which were later analysed for serum fatty acid concentrations. Despite good adherence to the study protocol - as reflected in increased concentrations of n-3 serum fatty acids - compared with placebo, the observed effects of both active treatments were minimal. The only finding of note revealed that supplementation with EPA-rich FO may reduce subjective mental fatigue at times of high cognitive demand, although further investigation is required. These findings, taken together with other recent reports of null effects, suggest that dietary supplementation with n-3 PUFA in healthy, normally developing and impairment-free populations is unlikely to result in cognitive enhancement.

  20. Differential Ratios of Omega Fatty Acids (AA/EPA+DHA Modulate Growth, Lipid Peroxidation and Expression of Tumor Regulatory MARBPs in Breast Cancer Cell Lines MCF7 and MDA-MB-231.

    Directory of Open Access Journals (Sweden)

    Prakash P Mansara

    Full Text Available Omega 3 (n3 and Omega 6 (n6 polyunsaturated fatty acids (PUFAs have been reported to exhibit opposing roles in cancer progression. Our objective was to determine whether different ratios of n6/n3 (AA/EPA+DHA FAs could modulate the cell viability, lipid peroxidation, total cellular fatty acid composition and expression of tumor regulatory Matrix Attachment Region binding proteins (MARBPs in breast cancer cell lines and in non-cancerous, MCF10A cells. Low ratios of n6/n3 (1:2.5, 1:4, 1:5, 1:10 FA decreased the viability and growth of MDA-MB-231 and MCF7 significantly compared to the non-cancerous cells (MCF10A. Contrarily, higher n6/n3 FA (2.5:1, 4:1, 5:1, 10:1 decreased the survival of both the cancerous and non-cancerous cell types. Lower ratios of n6/n3 selectively induced LPO in the breast cancer cells whereas the higher ratios induced in both cancerous and non-cancerous cell types. Interestingly, compared to higher n6/n3 FA ratios, lower ratios increased the expression of tumor suppressor MARBP, SMAR1 and decreased the expression of tumor activator Cux/CDP in both breast cancer and non-cancerous, MCF10A cells. Low n6/n3 FAs significantly increased SMAR1 expression which resulted into activation of p21WAF1/CIP1 in MDA-MB-231 and MCF7, the increase being ratio dependent in MDA-MB-231. These results suggest that increased intake of n3 fatty acids in our diet could help both in the prevention as well as management of breast cancer.

  1. Determination of EPA, DPA and DHA in Seal Oil by Gas Chromatography%气相色谱法测定海豹油中EPA、DPA、DHA含量的方法学研究

    Institute of Scientific and Technical Information of China (English)

    杨宜婷; 张晓燕; 区海燕

    2012-01-01

    Objective: To establish a method for determining co -3 polyunsaturated fatty acids including eicosapentaenoic acid (EPA), docosapentenoic acid (DPA) and docosahexaenoic acid (DHA) in seal oil. Methods: Fatty acids in seal oil were methyl esterified using a KOH-methanol solution. The applicability of the developed method was validated. Results: This method revealed an excellent liner relationship for methylated EPA, DPA and DHA in the concentration range of 10--2000μg/mL with recovery rate of 96.93%-- 103.38% and RSD less than 2%. Conclusion: This method is simple, rapid, accurate and suitable for the analysis of fatty acids in seal oil.%目的:建立气相色谱检测海豹油中EPA、DPA和DH。A的方法。方法:采用氢氧化钾-甲醇酯化法将海豹油中的脂肪酸快速、有效的转化成脂肪酸甲酯,并通过方法学实验验证其可行性。结果:EPA、DPA和DHA甲酯含量在10-2000lag/mL范围内,峰面积与EPA、DPA和DHA甲酯含量呈良好线性关系;加样回收率在96.93%~103.38%之间,相对标准偏差小于2%;结论:该方法可以快速、准确地同时测定EPA、DPA和DHA含量。

  2. 鱼油精炼副产物中EPA和DHA的回收工艺研究%Study on Recovering EPA and DHA from Fish Oil By-Product

    Institute of Scientific and Technical Information of China (English)

    方旭波; 蒋雅美; 龚戬芳; 陈小娥; 黄海华; 王庆和; 李振达

    2012-01-01

    以鱼油精炼副产物的皂脚和固脂为原料,采用酸催化乙酯工艺回收皂脚、碱催化乙酯工艺回收固脂,结果表明:酸催化回收皂脚时无水乙醇/脂肪酸为0.8 mL/g,硫酸/脂肪酸为0.09 mL/g,78℃下反应4h,鱼油乙酯化得率达到97%;碱催化回收固脂时无水乙醇用量0.6 mL/g,NaOH用量3%,78℃下反应3h,鱼油乙酯化得率达到94.5%.以酸、碱催化得到的乙酯化鱼油为原料,分别经二次三级分子蒸馏和一次尿素包合,产品中EPA、DHA的总质量分数分别可达到78.42%和76.15%.%Fish oil refining by - product of soap stock and solid lipid was used as the raw material, and the processes used were acid - catalyzed ester recovery process soap, and solid base - catalyzed ester resin recovery process. The results showed that when the acid - catalyzed recycling soap ethanol consumption 0. 8 ML/g, sulfuric acid concentration of 0.09 ML/g, and at 79℃ for 4 h,the fish oil ethyl ester rate is 97%. Recovery of solid lipid when the base -catalyzed ethanol consumption 0. 6 ML/g,NaOH concentration of 3% ,and at 79℃ for 3 h,the ester-ification rate of fish oil is up to 94. 5%. The acid - base catalyst with ethyl ester fish oil is used as raw material,a second three level and urea inclusion can produce EPA and DHA content of up to 78.42% and 76.15% respectively.

  3. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and gamma-linolenic acid (GLA) and contribution to normal cognitive function (ID 532) and maintenance of normal bone (ID 642, 697, 1552) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food constituents that are the subjects of the health claims are “omega-3 and omega-6 fatty acids (GLA)”, “gamma-linolenic acid + eicosapentaenoic acid (GLA+EPA)”, and “evening primrose oil...... and fish oil”. From the references provided, the Panel assumes that the food constituents that are the subject of the claims are the n-6 fatty acid gamma-linolenic acid (GLA) in evening primrose oil and the n-3 long-chain polyunsaturated fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA...

  4. 饲粮中添加DHA复合添加剂对蛋鸡生产性能和蛋品质的影响%Effects of DHA Compound Additives in Diets on Production Performance and Egg Quality of Laying Hens

    Institute of Scientific and Technical Information of China (English)

    彭瑛; 张彬; 占今舜; 李丽立; 曹建明; 罗锐; 高建武; 胡金杰; 罗佳捷; 范觉鑫

    2012-01-01

    In order to investigate influence of DHA compound additives in diets on egg quality and production performance, 96 Hy-Line layers were divided into 4 groups at random, and each group consisted of 6 cages with 4 laying hens per cage. Group I was control group and fed with basal diet. Groups II -IV were experimental groups and fed increasing levels of DHA compound additives at 2% ,4% and 8% respectively. Results showed that average laying rate, average egg shape index and average feed/egg ratio of all groups were not significantly different (P > 0. 05) ; the average egg mass of groups II - IV were found to be significantly higher than that of group I (P <0. 01) in the whole trial period. Average haugh unit of group IV was also significantly higher than that of group I in the first two weeks ( P < 0. 01) . In the third week, average haugh unit of group IV was significantly higher than that of the other three groups ( P < 0. 05 ). In the last two weeks, average contents of DHA in groups II - IV were significantly higher than those of group I (P < 0. 01 ) , whereas group IV was the highest. To sum up, the effects of DHA compound additive in diets can improve production performance and egg quality. According to the experiment results, the 8% DHA compound additive in diets is found to be the best approach.%试验选用96羽海兰灰蛋鸡随机分成4个处理,每个处理6个重复,每个重复4只鸡,试验期为28 d.第Ⅰ组为对照组,第Ⅱ~Ⅳ组为试验组,分别添加DHA复合添加剂为2%、4%和8%.旨在探讨蛋鸡饲粮中添加DHA复合添加剂对蛋鸡生产性能和蛋品质的影响.结果表明:1)在整个试验期,第Ⅱ~Ⅳ组与第Ⅰ组相比,蛋鸡的平均产蛋率和平均料蛋比无显著差异(P>0.05),平均蛋质量极显著提高(P<0.01).2)在整个试验期,平均蛋形指数第Ⅱ、Ⅲ、Ⅳ组与第Ⅰ组无显著差异(P>0.05);试验第1、2周,平均哈氏单位第Ⅳ组显著高于第Ⅰ组(P<0.05),试验第3

  5. DHA高产菌Schizochytrium sp.FJU-512的分离及其18S rRNA基因序列比较分析%ISOLATION OF SCHIZOCHYTRIUM SP. FJU-512 WITH HIGH YIELD OF DHA AND COMPARATIVE ANALYSIS ON ITS 18S rRNA GENE SEQUENCE

    Institute of Scientific and Technical Information of China (English)

    黄建忠; 江贤章

    2005-01-01

    采用松花粉垂钓法分离到一株Docosahexaenoic acid(DHA)高产菌FJU-512.该菌株DHA含量高(占总脂肪酸的56.24%),其它长链杂酸含量少(仅有docosapentaenoic acid,DPA),极具开发应用价值.高密度培养可获得33 gL-1生物量.该菌株行二分裂生长,没有分生胞子.对其18S rRNA基因进行了克隆测序并登录GenBank(AY758384).依据18S rRNA基因建立的系统进化树表明:该菌与Schizochytrium limacinum具有紧密的亲源关系.图7表2参29

  6. Effects of dietary LA and DHA supplementation on odd and branch-chain fatty acid composition of rumen protozoa in dairy cows%日粮添加亚油酸和二十二碳六烯酸对奶牛瘤胃原虫奇数碳链脂肪酸和支链脂肪酸的影响

    Institute of Scientific and Technical Information of China (English)

    王蕾; 王加启; 李发弟; 刘仕军; 哈斯; 梁松

    2010-01-01

    试验选取4头安装有永久性瘤胃、十二指肠和回肠瘘管的荷斯坦泌乳奶牛,采用4×4拉丁方试验设计,研究日粮中添加亚油酸(LA)和二十二碳六烯酸(DHA)对瘤胃原虫中奇数碳链脂肪酸和支链脂肪酸的影响.试验结果表明:奇数碳链脂肪酸和支链脂肪酸(OBCFA)是原虫脂肪酸的重要组成部分,与对照组相比,日粮中单独添加LA或DHA对瘤胃原虫中主要OBCFA的含量无显著影响,而当LA和DHA协同添加时C15∶0和C17∶0的含量显著降低(P<0.05),但对异构酸和反异构酸的含量无显著影响.

  7. 4种抗氧化剂对二十二碳六烯酸和二十碳五烯酸稳定性影响研究%Effects of 4 Kinds of Antioxidants on the Stability of DHA and EPA

    Institute of Scientific and Technical Information of China (English)

    王晓辉; 王文彤; 陈冠; 陶遵威

    2010-01-01

    目的:研究影响二十二碳六烯酸(DHA)和二十碳五烯酸(EPA)氧化发生的因素,寻找其高效的抗氧化剂.方法:以DHA、EPA质量为指标,温度、光照、时间为考察因素,采用正交试验法,优选对DHA、EPA氧化影响最小的条件;通过对样品添加红景天苷、丁基羟基茴香醚(BHA)、二丁基羟基甲苯(BHT)、维生素E 4种抗氧化剂,筛选最强抗氧化剂.结果:温度越低、光照越弱、时间越短,DHA、EPA氧化越慢;红景天苷对DHA、EPA的抗氧化作用最强.结论:通过降温、避光、减少储存时间、添加红景天苷的方法可以减弱DHA、EPA的氧化.

  8. Effect of DHA intervention of pregnant rats with intrauterine infection on inflammation state of cerebral tissues of neonatal rats%孕鼠DHA干预对宫内感染仔鼠脑组织炎症状态的影响

    Institute of Scientific and Technical Information of China (English)

    刘艳; 丰利芳; 徐三清; 罗小平

    2011-01-01

    目的:探讨宫内感染的孕鼠DHA干预后,对围产期炎症暴露的仔鼠脑组织炎症状态的影响.方法:本实验将孕鼠随机分为3组,①对照组:孕期第1天开始无菌生理盐水灌胃至分娩,于孕第17、18天腹腔注射无菌生理盐水0.6ml.②LPS组(模型组):无菌生理盐水灌胃,孕第17、18天连续两次腹腔注射LP3,计量为350 μg/kg.③LPS+ DHA组(干预组):孕期第1天开始给予DHA130 mg/kg灌胃至分娩,于孕第17、18天连续两次腹腔注射LPS,计量同②.取各组孕21天(G21),生后P1、P7、P14脑组织,用HE染色法观察不同时间点脑组织的病理改变,用免疫组化方法检测脑组织中0X42阳性小胶质细胞的表达情况.用Q - RT - PCR检测脑组织中细胞因子TNF -α的mRNA转录水平.结果:模型组脑白质早期可见水肿和形态较幼稚的细胞,甚至伴有局灶性出血,后期脑白质内神经纤维走向紊乱,组织疏松,细胞数减少.干预组早期脑白质可见较多水肿细胞,无明显出血灶和软化灶,P14时与对照组形态接近.与对照组相比:①模型组小胶质细胞0X42阳性表达在P14时仍较高(P<0.05);干预组至P7时亦较高(P<0.05),P14时无明显差异(P>0.05);干预组在各时间点与模型组比较均较低(P<0.05).②模型组TNF-αmRNA的表达在P7时仍较高(P<0.05),而在P14无显著差异(P>0.05);干预组TNF-αmR-NA的表达较模型组低直至P7 (P <0.05),而在P14无显著差异(P>0.05).结论:宫内LPS感染可导致胎儿脑组织炎性反应,孕期补充DHA能通过减少小胶质细胞的持续活化和炎症细胞因子的表达而抑制胎儿脑组织的炎症反应,促进脑损伤的恢复.%Objective: To explore the effect of DHA intervention of pregnant rats with intrauterine infection on inflammation state of cerebral tissues of neonatal rats exposure to perinatal inflammation. Methods: All the neonatal rates were divided into three groups randomly. The neonatal rats in

  9. DHA诱导小鼠乳癌4T1细胞凋亡及其对死亡受体蛋白表达的影响%APOPTOSIS OF MOUSE 4TI BREAST CANCER CELL INDUCED BY DHA AND ITS DFFECTS ON EXPRESSIONS OF DEATH RECEPTORS

    Institute of Scientific and Technical Information of China (English)

    隋英忠; 薛美兰; 葛银林; 张金玉; 何信佳

    2015-01-01

    目的 研究二十二碳六烯酸(DHA)对小鼠乳癌4T1细胞凋亡的诱导作用及其对死亡受体(DR)蛋白表达的影响.方法 体外培养4T1细胞,应用MTT方法观察不同浓度的DHA对4T1细胞增殖活力的影响;Annexin V/PI双染流式细胞术分析不同浓度DHA对4T1细胞凋亡率影响;采用Western blotting方法检测DR4、DR5和肿瘤坏死因子相关凋亡诱导配体(TRAIL)表达的变化.结果 采用不同浓度DHA处理后,4T1细胞增殖活力较对照组显著下降(F=84.62,q=16.54~25.31,P<0.05);细胞凋亡率明显增高(F=287.68,q=32.74~56.58,P<0.05);DR5和TRAIL表达均明显上调(F=64.75、149.72,q=9.32~51.59,P<0.05).结论 DHA可能通过DR途径抑制小鼠4T1乳癌细胞的生长,诱导其凋亡.

  10. Effect of Docosahexaenoic Acid (DHA) on Spinal Cord Injury.

    Science.gov (United States)

    Samaddar, Sreyashi

    2016-01-01

    Spinal cord injury (SCI) has become one of the most leading concerns in the past decade. Preclinical and research studies are now ongoing trying to understand the molecular mechanisms and develop treatment strategies for this neurodegenerative condition. In the last decade, researchers have deciphered few of the leading players that play a major role in worsening the condition. But till date none of these have been applied to the clinical treatment of patients with SCI. Here in this chapter I discuss about one of the dietary requirements that could ameliorate the condition of these patients. PMID:27651246

  11. Variación cuantitativa y cualitativa de la composición en ácidos grasos de Crypthecodinium cohnii en condiciones de supresión de nitrógeno

    Directory of Open Access Journals (Sweden)

    Carmona, L.

    2008-03-01

    Full Text Available Crypthecodinium cohnii is a heterotrophic dinoflagellate that can achieve a lipid content greater than 20% on dry weight. DHA can represent up to 40% of the total fatty acids. Our objective was to investigate the variation in fatty acid content when growth conditions are optimal compared with when there is limited growth, due to the absence of nitrogen in the media. Nitrogen-limited growth conditions caused the cells to stop dividing and accumulate lipids, principally as docohexanoic acid (DHA. Oxygen availability in the culture favoured DHA accumulation. In the cultures without nitrogen and with oxygen availability there was a lipid cell content 3,18 times higher than in the control condition (with nitrogen and air, and 2,25 higher than those without nitrogen and air. C. cohnii can be used as an optimal DHA source for the production of phytodiets in aquaculture.Crypthecodinium cohnii, es un dinoflagelado marino heterotrófico estricto, que puede alcanzar contenidos en lípidos superiores al 40% de su peso seco, representando el DHA más del 40% del conjunto total de ácidos grasos. El objetivo del presente trabajo fue el de establecer el perfil de variación de los ácidos grasos en condiciones de óptima disponibilidad de nutrientes y de supresión de nitrógeno. Los resultados muestran que en condiciones de supresión de nitrógeno las células dejan de dividirse y empiezan a acumular lípidos, especialmente ácido docosahexaenoico (DHA. La disponibilidad de oxígeno en el medio también favorece este proceso. En los cultivos sin nitrógeno y con disponibilidad de oxígeno se alcanzó un contenido celular de ácidos grasos 3,18 veces superior al alcanzado en la condición control (con nitrógeno y aire y 2,25 al observado en los cultivos sin nitrógeno no burbujeados. Crypthecodinium cohnii puede constituir una óptima fuente de DHA para la elaboración de fitodietas en acuicultura.

  12. 真菌发酵法生产EPA和DHA%PRODUCTION OF EPA AND DHA BY FUNGAL FERMENTATION

    Institute of Scientific and Technical Information of China (English)

    毛跟年; 张俊涛

    2005-01-01

    利用真菌发酵生产EPA和DHA已是目前研究的热点.综述了菌株种类、发酵培养基、发酵条件对EPA和DHA的合成产生的影响,探讨了工业化生产所面临的问题及解决途径.

  13. Contenidos de isomeros trans de los acidos grasos en productos carnicos (I). Embutidos

    OpenAIRE

    Hernández, N.; Codony Salcedo, Rafael; Rafecas Martínez, Magdalena; Boatella Riera, Josep

    1991-01-01

    En este trabajo se determinan los contenidos de isómeros trans de la fracción de los ácidos grasos, en muestras de embutidos diversos (total 48 muestras), mediante extracción previa de la fracción grasa y posterior análisis por cromatografía de gases capilar, con columna CP Sil 88. Los resultados muestran una gran variabilidad, entre 0,14 y 1,63% de C18:1 t y entre 0,37 y 2,14% de formas trans totales.

  14. Acid Deposition Maps in Spain; Mapas de Deposito Acido en Espana

    Energy Technology Data Exchange (ETDEWEB)

    Artinano, B.; Cabal, H.; Garcia, C.

    1998-07-01

    Animal and monthly deposition velocity and total sulfur deposition maps have been performed for the peninsular Spain for 1992 by using the inferential method. To do this, updated databases with high space and time resolution, for land uses (CORINE) and meteorological information from analysis modelling for the same year, have been utilized. The final result are deposition maps in a 5x5 Km{sup 2} grid which allow to assess the methodology used in Europe to obtain the maps of excedances over the critical loads of pollutants. (Author) 32 refs.

  15. Terpenes and fatty acids from Dipteryx lacunifera Ducke; Terpenos e acidos graxos de Dipteryx lacunifera Ducke

    Energy Technology Data Exchange (ETDEWEB)

    Vieira Junior, Gerardo Magela; Silva, Hilris Rocha e; Bittencourt, Thais Chaves; Chaves, Mariana H. [Universidade Federal do Piaui (UFPI), Teresina (Brazil). Dept. de Quimica]. E-mail: mariana@ufpi.br; Simone, Carlos Alberto de [Universidade Federal do Alagoas (UFAL), Maceio, AL (Brazil). Inst. de Quimica e Biotecnologia

    2007-07-01

    This paper describes the isolation of the furanocassane-type diterpene, named vinhaticoic acid, along with b-farnesene and spatulenol from fruit shells of D. lacunifera. Structural determinations were accomplished by chemical derivatization and spectral analysis, including 1D and 2D NMR and X-ray crystallography. The fatty portion was extracted from the fruit kernels, transesterfied and analysed by HRGC/MS. Oleic acid (75.8 +- 4.3%) was the major component. Essential oil extracted from the fruit shells of D. lacunifera was analysed by HRGC/MS and nine sesquiterpenes were identified; beta-farnesene (48.6%) and spatulenol (21.61%) were the major constituents. (author)

  16. Acido, ergo sum: Holger Hydén--the neuroscientist in Cortázar's Hopscotch.

    Science.gov (United States)

    Delgado, Guillermo; Estañol, Bruno

    2013-06-01

    The fictional Italian author Morelli is throughout the novel "Hopscotch" (1963) Julio Cortázar's alter ego. This character proposes an unoriginal literary hypothesis in chapter 62. There is an allusion to a particular Swedish that 'is working on a chemical theory of thought.' The Swedish neuroscientist under analysis is Holger Hydén (1917-2000), by then professor and chairman of the Department of Histology at the University of Göteborg. Hydén, who was the first to work in neurobiological micromethods, is mentioned by Morelli due to his participation in a symposium held at the end of January 1961, in San Francisco. His pioneering work will never be completely forgotten, because Hydén's neuroscientific legacy lives and will live in Cortázar's "Hopscotch".

  17. Isolation of deoxyribonucleic acids (A Review); Aislamiento de los acidos desoxiribonucleicos. Revision Bibliografica

    Energy Technology Data Exchange (ETDEWEB)

    Garcia de Pineda, M. de

    1974-07-01

    The criteria of choice in this Review have been to gather some of the last advances in the methodology of DNAs isolation; also the description of the generally accepted procedures has been emphasized. Only papers published before March 1974 are reviewed, because this work has been finished during this month. (Author) 109 refs.

  18. Acido, ergo sum: Holger Hydén--the neuroscientist in Cortázar's Hopscotch.

    Science.gov (United States)

    Delgado, Guillermo; Estañol, Bruno

    2013-06-01

    The fictional Italian author Morelli is throughout the novel "Hopscotch" (1963) Julio Cortázar's alter ego. This character proposes an unoriginal literary hypothesis in chapter 62. There is an allusion to a particular Swedish that 'is working on a chemical theory of thought.' The Swedish neuroscientist under analysis is Holger Hydén (1917-2000), by then professor and chairman of the Department of Histology at the University of Göteborg. Hydén, who was the first to work in neurobiological micromethods, is mentioned by Morelli due to his participation in a symposium held at the end of January 1961, in San Francisco. His pioneering work will never be completely forgotten, because Hydén's neuroscientific legacy lives and will live in Cortázar's "Hopscotch". PMID:23828537

  19. Acid Deposition Maps in Spain; Mapas de Deposito Acido en Espana

    Energy Technology Data Exchange (ETDEWEB)

    Artinano, B.; Cabal, H.; Garcia, C. [CIEMAT. Madrid (Spain)

    1998-12-31

    Annual and monthly deposition velocity and total sulfur deposition maps have been performed for the peninsular Spain for 1992 by using the inferential method. To de this, updated database with high space and time resolution, for land uses (CORINE) and meteorological information from analysis modelling for the same year, have been utilized. The final result are deposition maps in a 5 x 5 km``2 grid which allow to assess the methodology used in Europe to obtain the maps of excedance over the critical loads of pollutants. (Author) 32 refs.

  20. Copper corrosion originated by volatile organic acid vapours; Corrosion del cobre por acidos organicos volatiles

    Energy Technology Data Exchange (ETDEWEB)

    Cano, E.; Polo, J. L.; Kong, D. Y.; Mora, E. M.; Lopez-Caballero, J. A.; Bastidas, J. M.

    2004-07-01

    The corrosion of copper in the presence of volatile organic acids is frequent. Thus, for example, it is known that failures by corrosion of the copper tubes take place in the air conditioning equipment, caused by volatile organic acids emitted by oils used in their manufacturing. Another frequent case is the corrosion of copper objects caused by the acids emitted by the materials used in packing, wood and resins, amongst others. This communication presents the corrosion results of copper exposed to 100% relative humidity and different concentrations (10-300ppm) of formic (HCOOH), acetic (CH{sub 3}COOH), propionic (CH{sub 3}CH{sub 2}COOH) and butyric (CH{sub 3}(CH{sub 2}){sub 2}COOH) acid vapours, for short exposure times. the techniques used were gravimetric analysis, scanning electron microscopy (SEM) and X-ray diffraction (XRD). (Author) 9 refs.

  1. Naphthenic acid characterization and distribution in crude oils; Caracterizacao e distribuicao de acidos naftenicos em petroleos

    Energy Technology Data Exchange (ETDEWEB)

    Guimaraes, Regina C.L.; Gomes, Alexandre de O. [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil)

    2008-07-01

    Naphthenic acid corrosion was observed for the first time during the distillation process of some kind of crude oils in 1920. Recent reports about naphthenic acid corrosion have been found in China, India, Venezuela, Eastern Europe, Russia and the USA. In Brazil, heavy and acid crude oil processing is rising. Some brazilian crude oils have TAN around 3,0 mg KOH/g. The presence of relatively high levels of naphthenic acids in crude oils is a bane of petroleum refiners; and more recently, of producers as well, who have reported problems during production with calcium and sodium naphthenate . Essentially, these acids which are found to greater or lesser extent in virtually all crude oils are corrosive and tend to cause equipment failures, lead to high maintenance costs and may pose environmental disposal problems. In order to give these information to PETROBRAS, The Research and Development Center of PETROBRAS (CENPES) has been working in house and with brazilian universities developing analytical technicians to know better molecular structures and distribution of these acids compounds in crude oils. This work presents the actual methods and some results from these developments. (author)

  2. Quantification of uranyl in presence of citric acid; Cuantificacion de uranilo en presencia de acido citrico

    Energy Technology Data Exchange (ETDEWEB)

    Garcia G, N.; Barrera D, C.E. [UAEM, Facultad de Quimica, 50000 Toluca, Estado de Mexico (Mexico); Ordonez R, E. [ININ, 52750 La Marquesa, Estado de Mexico (Mexico)]. e-mail: nidgg@yahoo.com.mx

    2007-07-01

    To determine the influence that has the organic matter of the soil on the uranyl sorption on some solids is necessary to have a detection technique and quantification of uranyl that it is reliable and sufficiently quick in the obtaining of results. For that in this work, it intends to carry out the uranyl quantification in presence of citric acid modifying the Fluorescence induced by UV-Vis radiation technique. Since the uranyl ion is very sensitive to the medium that contains it, (speciation, pH, ionic forces, etc.) it was necessary to develop an analysis technique that stands out the fluorescence of uranyl ion avoiding the out one that produce the organic acids. (Author)

  3. Avaliacao da seguranca de diferentes doses de suplementos de acido folico em mulheres do Brasil

    Directory of Open Access Journals (Sweden)

    Quenia dos Santos

    2013-10-01

    Full Text Available OBJETIVO Avaliar a distribuição da ingestão de ácido fólico e a segurança de diferentes doses de suplementos em mulheres em idade reprodutiva. MÉTODOS Foram utilizados dados de consumo a partir de dois dias não consecutivos de registro alimentar de 6.837 mulheres em idade reprodutiva (19 a 40 anos participantes do Inquérito Nacional de Alimentação, módulo da Pesquisa de Orçamentos Familiares 2008-2009. Médias e percentis de consumo habitual de folato natural e ácido fólico foram estimados utilizando o método do National Cancer Institute . Cinco cenários foram simulados somando-se diferentes doses diárias de fortificação (400 mcg, 500 mcg, 600 mcg, 700 mcg e 800 mcg ao ácido fólico oriundo dos alimentos consumidos pelas mulheres. Comparou-se o total de ácido fólico (dieta + suplemento com o nível máximo de ingestão tolerável (UL = 1.000 mcg para definir a dose segura de suplementação. RESULTADOS Mulheres com ingestão habitual de ácido fólico acima do nível máximo de ingestão tolerável foram observadas para doses de suplemento de 800 mcg (7,0% das mulheres. Abaixo desse valor, qualquer dose de suplementação mostrou-se segura. CONCLUSÕES O uso de suplementos de até 700 mcg de ácido fólico mostrou-se seguro.

  4. Long-chain polyunsaturated fatty acid concentration in patients with inborn errors of metabolism Concentración de ácidos grasos poliinsaturados de cadena larga en pacientes con errores innatos del metabolismo

    Directory of Open Access Journals (Sweden)

    M.ª A. Vilaseca

    2011-02-01

    Full Text Available Introduction: Long-chain polyunsaturated fatty acid (LCPUFA can be provided by diet (fatty fish, eggs, viscera and human milk or synthetised from essential fatty acids linoleic and α-linolenic acids through the microsomal pathway. However, endogenous LCPUFA synthesis is rather low, especially for docosahexaenoic (DHA, and seems insufficient to achieve normal DHA values in individuals devoid of preformed dietary supply. Inborn errors of metabolism (IEMs are therefore diseases with a special risk for LCPUFA deficient status. Aim: Our aim was to evaluate LCPUFA status in 132 patients with different IEMs. Methods: We performed a cross-sectional study of plasma and erythrocyte LCPUFA composition of 63 patients with IEMs treated with protein-restricted diets compared with data from 69 patients with IEMs on protein-unrestricted diets, and 43 own reference values. Results: Erythrocyte and plasma DHA and arachidonic acid concentrations were significantly decreased in patients treated with protein-restriction compared with those on protein-unrestricted diets and with our reference values (p Introducción: Los ácidos grasos poliinsaturados de cadena larga (LCPUFA pueden ser suministrados por la dieta o sintetizados a partir de los ácidos grasos esenciales, linoleico y α-linolénico. La síntesis endógena de LCPUFA es escasa, especialmente la de ácido docosahe-xaenoico (DHA, e insuficiente para alcanzar los valores normales de DHA en individuos que carecen de un suministro dietético de dichos ácidos preformados. Por ello, los errores innatos del metabolismo (IEM son enfermedades con riesgo especial de deficiencia de LCPUFAs. Objetivos: Evaluar el estado de LCPUFA en 132 pacientes con diferentes IEMs. Métodos: Estudio transversal de LCPUFA en plasma y eritrocitos de 63 pacientes con IEMs tratados con dieta restringida en proteínas comparados con 69 pacientes con IEMs con una dieta libre y 43 valores de referencia. Resultados: Las concentraciones de

  5. Fatty acids and astaxanthin composition of two edible native Mexican crayfish Cambarellus (C. montezumae and Procambarus (M. bouvieri

    Directory of Open Access Journals (Sweden)

    Coral-Hinostroza, G.

    2016-09-01

    Full Text Available The content and composition of the fatty acids (FAs and astaxanthin (AST in the edible forms of crayfish: the whole animal of Cambarellus (C. montezumae, and the tail meat (TM of Procambarus (M. bouvieri were determined by GC and HPLC. The exoskeleton (EXK of P. (M. bouvieri was also studied. Unsaturated FAs, and mostly oleic acid (C18:1 n-9, were predominant in both edible forms. The contents of the polyunsaturated eicosapentaenoic (C20:5 n-3, EPA, arachidonic (C20:4 n-6, ARA and docosahexaenoic acid (C22:6 n-3, DHA, were higher in the TM of P. (M. bouvieri than in the complete C. (C. montezumae (p -1 and 66.3 ± 3.91 μg·g-1, and were composed mainly of AST ( > 79.50%. AST esters were enriched with saturated FAs in C. (C. montezumae and with PUFAs in EXK of P. (M. bouvieri. We conclude that both C. (C. montezumae and the TM of P. (M. bouvieri are traditional foods rich in n-3 PUFAs and C. (C. montezumae in AST. The EXK of P. (M. bouvieri is a rich potential source of AST, n-3 PUFAs, and the combination AST-DHA.Se determinó por GC y HPLC el contenido y composición de ácidos grasos (AGs y astaxantina (AST, en dos formas comestibles de acocil: el animal completo de Cambarellus (C. montezumae, y el músculo de la cola (MC de Procambarus (M. bouvieri. Adicionalmente, se estudió el exosqueleto (EXK de P. (M. bouvieri. En ambas formas comestibles predominaron los AGs insaturados. Los contenidos de ácido eicosapentaenoico (C20:5 n-3, EPA, araquidónico (C20:4 n-6, ARA y docosahexaenoico (C22: 6 n-3, DHA, fueron mayores en el MC que en C. (C montezumae (p -1 a 66.3 ± 3.9 μg·g-1, con predominancia de AST ( > 79.50%. Los ésteres de AST en C. (C. montezumae fueron enriquecidos con AGs saturados mientras que los del EXK de P. (M. bouvieri con AGs poliinsaturados. Se concluyó que tanto C. (C. montezumae como el MC de P. (M. bouvieri, son alimentos tradicionales ricos en PUFAs n-3, y C. (C. montezumae en AST. El EXK de P. (M. bouvieri abunda en

  6. Modificazioni dei livelli di acido arachidonico e vitamina A in relazione alla attività anticancerogena attribuita all'apporto alimentare di burro naturalmente arricchito in acido linoleico a dieni coniugati (CLA)

    OpenAIRE

    Banni, Stefano; Angioni, E.; Carta, G.; Dessi, M. Assunta; Melis, M. Paola; Murru, E.; Scanu, P.; Spada, Sebastiano; Vargiolu, Silvana; Corongiu, Francesco P.

    2000-01-01

    Considerable attention has recently been directed to the naturally occurring trace fatty acid conjugated linoleic acid (CLA), based on its dual ability to act both as a preventive and therapeutic agent in a number of rodent and human tumor model systems. CLA, which is synthesized by rumen bacteria, is found in meat and dairy products, and is of particular interest since it is effective at low levels in the diet. Our research interest is to explore whether the mechanism of the anticarcinogenic...

  7. Estudios Cinéticos de la Disolución de Hidroboracita en Soluciones de Acido Clorhídrico y Acido Sulfúrico Dissolution Kinetics of Hydroboracite in Hydrochloric Acid and Sulphuric Acid Solutions

    Directory of Open Access Journals (Sweden)

    Graciela V Morales

    2008-01-01

    Full Text Available Se realizan estudios cinéticos de la disolución de hidroboracita en soluciones de ácido clorhídrico y ácido sulfúrico en un reactor tanque agitado discontinuo de acero inoxidable, a presión atmosférica. Se investiga el efecto de la temperatura, el tamaño de partícula, la relación sólido/líquido y la concentración de ácido sobre la velocidad de disolución. Los resultados experimentales permiten observar que la velocidad de disolución aumenta con el incremento de la concentración del ácido y de la temperatura, y disminuye con el aumento del tamaño de partícula y con la relación sólido/líquido. La energía de activación aparente calculada para la disolución de hidroboracita en soluciones de ácido clorhídrico es 44.9 kJ/mol, lo cual no permite inferir cual es la etapa controlante del proceso; mientras que la energía de activación aparente calculada para la disolución de hidroboracita en soluciones de ácido sulfúrico es 14.6 kJ/mol, lo cual permite inferir que el proceso ocurre con control difusivo.The dissolution kinetics of hydroboracite in hydrochloric acid and sulphuric acid solutions was investigated. The experiments were carried out in a steel batch reactor equipped with a mechanical stirrer, at atmospheric pressure. The effect of reaction temperature, particle size, solid/liquid ratio and acid concentration on the dissolution rate was determined. It was found that the dissolution rate increased with increasing the acid concentration and reaction temperature. However, the increase of particle size and solid/liquid ratio decreased the dissolution rate. The activation energy for the hydroboracite dissolution in hydrochloric acid was calculated as 44.9 kJ/mol, which does not permit to know the process controlling step. The activation energy for the hydroboracite dissolution in sulphuric acid was found to be 14.6 kJ/mol, which indicates that the dissolution is a diffusion controlled process.

  8. Effects of acido-basic support properties on the catalytic hydrogenation of acetylene on gold nano-particles

    Science.gov (United States)

    Manda, Abdullah Ahmed

    Metallic gold nanoparticles supported on gamma-Al2O 3 and magnesia-alumina mixed oxide, with different magnesia content have been prepared by sol-gel method and characterized by different techniques (inductive coupled plasma-mass spectroscopy (ICP-MS), XRD, BET surface area analysis, transmission electron microscopy (TEM), CO2 and NH 3 temperature programmed desorption (TPD), H2 temperature programmed reduction (TPR) and FTIR of adsorbed CO2). Such systems were found to produce catalysts with controllable acidity, varying from catalyst possessing large density of acidic and low density of basic sites, others with acidic and basic sites of equal strength and density, and others with large basic and low acid sites densities, respectively. The catalytic assessment of the generated acidity was carried out using 2-propanol decomposition as a test reaction. The results obtained indicate that the presence of magnesia and reduced gold nanopartilces has imparted the catalysts, 1%Au/4%Mg-Al 2O3 and 1%Au/8%Mg-Al2O3, with significant base-catalytic properties. Acetylene hydrogenation and formation of coke deposits were investigated on a gold catalyst supported on gamma-Al2O3 and gold supported on alumina-magnisia mixed oxide with different gold content; 1%Au/gamma-Al 2O3, 1%Au/15%Mg-Al2O3, 2%Au/15%Mg-Al 2O3 and 4%Au/15%Mg-Al2O3. The effect of the H2/C2H2 ratio was studied over a range of values. The catalytic activity and selectivity towards ethylene and other products were investigated at different reaction temperatures. Acetylene hydrogenation was investigated in the presence and absence of ethylene in stream. It is investigated that the adsorption of the triple bond is preferred over the double bond and during selective catalytic (SCR) of C2H2 the two hydrocarbons do not compete for the same adsorption sites. The deactivation of catalysts was studied by temperature programmed oxidation (TPO). Higher content of coke over 1%Au/Al2O3 catalyst was investigated in contrast to the other catalysts in which the basisity of magnesia-alumina supports could have played an important role in inhibiting the acidic carbon formation by possessing smaller number and/or weaker acid sites.

  9. Revisión de los niveles de acidos grasos trans encontrados en distintos tipos de alimentos

    Directory of Open Access Journals (Sweden)

    Vicario, I. M.

    2007-03-01

    Full Text Available In this paper a comprehensive review of trans-fatty acid levels reported in different types of food products is offered. The lowest levels are found in some types of bread (0,1% while the highest levels (up to 40% are found in fried potatoes. Some foods like shortenings, cakes and pastries may have up to 30% of these fatty acids. In milk and dairy products the reported levels are between 1.50% and 5.20%, in meat and derived products between 0.20% and 21,30%; in chocolates from 0% to 15, 70%. The greatest variability is found in margarines, where a variety has been reported from “trans-free products” to products containing up to 34. 30%. In relation to the trans-fatty acid profile, 18:1t is the most abundant fatty acid found in the majority of foods, followed by C18:2t, C18:3t, C18:1t y C14:1t. Due to the negative health effects of these fatty acids, there is a trend to reduce their levels in food products and to include them in the nutritional label of food packaging.En este trabajo se realiza una exhaustiva revisión bibliográfica de los contenidos en grasos trans en alimentos de distinto origen. Los niveles más bajos se encuentran en algunos tipos de pan (0,10%, y los más elevados (hasta 40%, en algunas muestras de patatas fritas. Alimentos tales como shortenings, algunos tipos de galletas y bollería contienen hasta el 30% de dichos ácidos grasos. En las leches y derivados se encuentran cantidades entre 1,50% y 5,20%; en carnes y derivados entre 0,20% y 21,30%; en chocolates desde cantidades nulas hasta 15,70%. La mayor variabilidad se registra en margarinas, entre las que se encuentran desde las “libres de trans”, hasta algunas con 34,30% de estos ácidos grasos. En cuanto a los distintos isómeros, el mayoritario es el C18:1t seguido del C18:2t, C18:3t, C18:1t y C14:1t. Debido a los efectos desfavorables que presentan sobre la salud se observa en los últimos años una tendencia a reducir este tipo de ácidos grasos en algunos alimentos y a incluirlos en el etiquetado nutricional de los alimentos.

  10. Contenidos de isomeros trans de los acidos grasos en productos carnicos. (III) Tejido adiposo y grasa intramuscular de vacuno

    OpenAIRE

    Lluch, M. C.; Roca de Vinyals, M.; Parcerisa Egea, Javier; Guardiola Ibarz, Francesc; Codony Salcedo, Rafael; Rafecas Martínez, Magdalena; Boatella Riera, Josep

    1993-01-01

    Se presentan los resultados obtenidos para la determinación de ácidos grasos en una serie de muestras de tejidos subcutáneo y muscular, procedentes de canales de vacuno, por aplicación de la cromatografía en fase gaseosa, para los que se obtuvieron unos valores medios de 58.7% de ácidos saturados, 39.1 % de monoinsaturados y 2.7% de polinsaturados, en el tejido adiposo, y de 44.7% de saturados, 46.1% de monoinsaturados y 9.4% de polinsaturados, en el tejido muscular. Los contenidos de ácidos ...

  11. New heterogeneous acid catalysts in the synthesis of biodiesel; Estudo de novos catalisadores heterogeneous acidos na sintese de biodiesel

    Energy Technology Data Exchange (ETDEWEB)

    Soldi, Rafael A.; Cesar-Oliveira, Maria Aparecida F. [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Dept. de Quimica. Lab. de Polimeros Sinteticos], e-mail: mafco@quimica.ufpr.br; Oliveira, Angelo R.S.; Ramos, Luiz P. [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Dept. de Quimica. Centro de Pesquisa em Quimica Aplicada (CEPESQ)

    2007-07-01

    In this work, sulfonated polystyrene compounds (PSS) were synthesized from linear polystyrene (PS). Several methods and experimental conditions were investigated for the sulfonation of PS, producing catalytically active polymeric materials with sulfonation degrees in the range of 5.0-6.2 mmol -SO{sub 3}H/g of dry polymer. The performance of these catalysts was evaluated in transesterification reactions of beef tallow and vegetable oils with ethanol and methanol. For the sake of comparison, the same reaction conditions employed for the PSS catalysts were also used for an Amberlyst 15 (3,7 mmol SO{sub 3}H/g - Aldrich). The PSS samples were shown to be insoluble in the reaction media, leading to conversion rates of 85%, 75% and 80% of the refined soybean oil, beef tallow and crude corn oil in to ethyl esters, respectively, and 94% of the refined soybean oil methyl esters. Amberlyst 15 was studied as an alternative to the process, but its conversion rate to alkyl esters was very low in the employed conditions. These results demonstrated that our synthetic PSS materials have a great potential to act as heterogeneous catalysts for transesterification. (author)

  12. Acido, ergo sum: Holger Hydén – the neuroscientist in Cortázar's Hopscotch

    Directory of Open Access Journals (Sweden)

    Guillermo Delgado

    2013-06-01

    Full Text Available The fictional Italian author Morelli is throughout the novel “Hopscotch” (1963 Julio Cortázar's alter ego. This character proposes an unoriginal literary hypothesis in chapter 62. There is an allusion to a particular Swedish that ‘is working on a chemical theory of thought.’ The Swedish neuroscientist under analysis is Holger Hydén (1917-2000, by then professor and chairman of the Department of Histology at the University of Göteborg. Hydén, who was the first to work in neurobiological micromethods, is mentioned by Morelli due to his participation in a symposium held at the end of January 1961, in San Francisco. His pioneering work will never be completely forgotten, because Hydén's neuroscientific legacy lives and will live in Cortázar's “Hopscotch”.

  13. Bacterias halotolerantes/alcalofilas productoras de acido indol acético (AIA asociadas a Arthrospira platensis (Cyanophyceae

    Directory of Open Access Journals (Sweden)

    Liliana Cecilia Gómez Gómez

    2012-08-01

    Full Text Available Título en ingles: Halotolerant alkalophilic and indolacetic acid producing acid producing bacteria associated with Arthrospira platensis (Cyanophyceae Resumen: Este trabajo tuvo como propósito contribuir al conocimiento de la interacción entre la cianobacteria alcalófila Arthrospira platensis y las bacterias que crecen asociadas a su mucilago. Se desarrolló un  medio de cultivo heterotrófico en el cual se aislaron cinco cepas bacterianas asociadas a un monocultivo de A. platensis. Se determinó la capacidad de estas cinco cepas para producir ácido 3- indol acético (AIA. La tipificación molecular de los aislamientos bacterianos permitió identificarlos como Exiguobacterium aurantiacum str. DSM 20416, Xanthomonas sp. ML-122, Halomonas sp. Ap-5, Bacillus okhensis str.  Kh10-101, Indibacter alkaliphilus, type str. LW1T; todas las cepas bacterianas obtenidas  son halotolerantes, alcalófilas y productoras de AIA. Los resultados aportan evidencia para sugerir una interacción benéfica entre A. platensis y sus bacterias asociadas,  quizá como estrategia evolutiva  de cooperación para desarrollarse en  un ambiente hipersalino.    Palabras claves: Bacillus okhensis, Exiguobacterium aurantiacum, Halomonas sp., Indibacter alkaliphilus. Xanthomonas sp. Abstract: The aim of this study was contribute to knowledge over alkalophilic cianobacteryum  Arthrospira platensis  and their  interaction with some associated bacteria growing in their mucilage. Heterotrophic culture medium was designed, in this medium were isolated five bacterial strains associated to single culture of A. platensis. It was measured the 3-indol acetic acid (IAA production by these bacterial strains. Molecular typing allowed identify these bacterial strains like Exiguobacterium aurantiacum str. DSM 20416, Xanthomonas sp. ML-122, Halomonas sp. Ap-5, Bacillus okhensis str. Kh10-101, Indibacter alkaliphilus, type str. LW1T; all these bacteria are halotolerant, alkalophilic and IAA producer.  The findings allow suggest a beneficial interaction between A. platensis and their associated bacteria, maybe as evolutionary strategy of cooperation to grow and develop in  hypersaline environments. Key words: Bacillus okhensis, Exiguobacterium aurantiacum, Halomonas sp., Indibacter alkaliphilus.Xanthomonas sp.

  14. Producción de acido láctico por Lactobacillus plantarum L10 en cultivos batch y continuo

    Directory of Open Access Journals (Sweden)

    Waldir Estela

    2013-04-01

    Full Text Available Se ha ensayado a escala de laboratorio la cepa Lactobacillus plantarum L10, para la producción de ácido láctico en cultivos batch y continuo; además se ha optimizado la composición del medio y las condiciones de cultivo para este propósito. Los mejores parámetros de producción de ácido láctico encontrados en cultivo batch fueron los siguientes: YP/S 86,1%; PP 5,4 g/L/h; unido a YX/S 13,2%; PX 1,2 g/L/h y μ = 0,2 h-1, el cultivo se ha llevado a cabo en un medio conteniendo glucosa 70 g/L; extracto de levadura 12,1 g/L; KH2PO4 1,2 g/L; (NH42HPO4 1,2 g/L; citrato de amonio 3,0 g/L; MgSO4. 7H2O 0,3 g/L y MnSO4. 4H2O 0,03 g/L. Así mismo los mejores parámetros de producción de ácido láctico encontrados en cultivo continuo fueron los siguientes: YP/S 96%; P´P 6,0 g/L/h; unido a YX/S 19 %; P´X 1,2 g/L/h; y tasa de dilución (D 0,46 h-1.

  15. Bacterias halotolerantes/alcalofilas productoras de acido indol acético (aia) asociadas a arthrospira platensis (cyanophyceae)

    OpenAIRE

    Liliana Cecilia Gómez Gómez; Nelson Valero Valero; Ever Morales Avendaño

    2012-01-01

    Título en ingles: Halotolerant alkalophilic and indolacetic acid producing acid producing bacteria associated with Arthrospira platensis (Cyanophyceae) Resumen: Este trabajo tuvo como propósito contribuir al conocimiento de la interacción entre la cianobacteria alcalófila Arthrospira platensis y las bacterias que crecen asociadas a su mucilago. Se desarrolló un  medio de cultivo heterotrófico en el cual se aislaron cinco cepas bacterianas asociadas a un monocultivo de A. platensis. Se determi...

  16. Gas chromatography of alkylphosphonic and dialkyl phosphinic acids; Cromatografia en fase gaseosa de acidos alquifosfonicos y dialquilfosinicos

    Energy Technology Data Exchange (ETDEWEB)

    Gasco Sanchez, L.; Barrera Peniero, R.; Ramirez Caceres, A.; Marin Munoz, M.

    1978-07-01

    After carrying out an optimization study on the separation conditions for the TMSr- derivatives, of the hexyl-, cyclohexyl-, heptyl-, and octyl-phosphonic acids; dihexyl dicyclohexyl-, heptyl-, and octyl-phosphinic acids, and dioctyl phosphine oxide, their retention indices (I) at two temperatures and on the OV-1 and OV-17 stationary phase were determined. Correlations between I and molecular structure were established. Calibration factors of these compounds in the flame ionization detector were studied, and the results analyzed taking into account the variables affecting the quantitative results, These results were unbiased but they had a lower precision than that usually achievable in gas chromatography. (Author) 24 refs.

  17. Polyunsaturated fatty acids in amyotrophic lateral sclerosis: role of DHA, peroxidative modifications and sexual dimorphism

    OpenAIRE

    Cacabelos Barral, Daniel

    2014-01-01

    In the present work we focus into the potential relevance of PUFAs in some models and human samples from patients suffering amyotrophic lateral sclerosis (ALS). Due to its pathological implication, oxidative stress was our first goal. We started from simple oxidative methodology screening to search for an antioxidant substance (among 21 different candidates) available in a Mediterranean diet. The results demonstrated high heterogeneity in carbonyl (measured by DNP) accumulation...

  18. DHA-Rich Tuna Oil Effectively Suppresses Allergic Symptoms in Mice Allergic to Whey or Peanut

    NARCIS (Netherlands)

    van den Elsen, Lieke Wj; Bol-Schoenmakers, Marianne; van Esch, Betty Cam; Hofman, Gerard A; van de Heijning, Bert Jm; Pieters, Raymond H; Smit, Joost J; Garssen, Johan; Willemsen, Linette Em

    2014-01-01

    BACKGROUND: Supplementation with long-chain n-3 polyunsaturated fatty acids (LCPUFAs) has been found to reduce the development of allergic disease. OBJECTIVE: The aim of this study was to compare the effectiveness of fish oil diets rich in eicosapentaenoic acid (20:5n-3; EPA) or docosahexaenoic acid

  19. DHA- and EPA-derived resolvins, protectins, and maresins in airway inflammation.

    Science.gov (United States)

    Duvall, Melody G; Levy, Bruce D

    2016-08-15

    Essential fatty acids can serve as important regulators of inflammation. A new window into mechanisms for the resolution of inflammation was opened with the identification and structural elucidation of mediators derived from these fatty acids with pro-resolving capacity. Inflammation is necessary to ensure the continued health of the organism after an insult or injury; however, unrestrained inflammation can lead to injury "from within" and chronic changes that may prove both morbid and fatal. The resolution phase of inflammation, once thought to be a passive event, is now known to be a highly regulated, active, and complex program that terminates the inflammatory response once the threat has been contained. Specialized pro-resolving mediators (SPMs) are biosynthesized from omega-3 essential fatty acids to resolvins, protectins, and maresins and from omega-6 fatty acids to lipoxins. Through cell-specific actions mediated through select receptors, these SPMs are potent regulators of neutrophil infiltration, cytokine and chemokine production, and clearance of apoptotic neutrophils by macrophages, promoting a return to tissue homeostasis. This process appears to be defective in several common human lung diseases, such as asthma and COPD, which are characterized by chronic unrestrained inflammation and significant associated morbidity. Here, we highlight translational research in animal models of disease and with human subjects that sheds light on this rapidly evolving area of science and review the molecular and cellular components of the resolution of lung inflammation. PMID:26546247

  20. Ácidos grasos omega-3 y prevención cardiovascular

    Directory of Open Access Journals (Sweden)

    Julio César Fernández Travieso

    2010-01-01

    Full Text Available Las enfermedades cardiovasculares (ECV constituyen la principal causa de muerte en los países desarrollados y su incidencia va en aumento en los países en desarrollo por lo que se estima que superarán la mortalidad por causas infecciosas en los próximos años. Por ello, es de máxima prioridad desarrollar estrategias encaminadas a identificar las causas de las ECV y cómo prevenir o paliar sus consecuencias. El éxito de cualquier medida preventiva depende en gran parte del conocimiento de los factores de riesgo y del impacto que su modificación pudiera tener sobre la progresión de la enfermedad. Los estudios experimentales, epidemiológicos y de intervención han demostrado los efectos beneficiosos cardiovasculares del consumo de ácidos grasos omega-3 [ácidos eicosapentaenoico (EPA y docosahexaenoico (DHA], los cuales han sido atribuidos a sus efectos antiateroscleróticos, antitrombóticos, antiarrítmicos y antiinflamatorios. En la presente reseña se hace una breve revisión de la estructura química de los ácidos grasos omega-3, las acciones farmacológicas y los mecanismos por los cuales estos ácidos pueden reducir el riesgo cardiovascular, se analizan las evidencias clínicas que soportan su rol cardioprotector. También, se comenta brevemente el perfil de seguridad de estos agentes y las recomendaciones de las guías internacionales relativas a su uso en la prevención y manejo de la enfermedad cardiovascular. Se concluye que teniendo en cuenta los beneficios que aportan para la prevención y tratamiento de las enfermedades cardiovasculares se hace necesario incrementar su consumo a través de la dieta o con suplementos.

  1. Evaluación físico-química de aceite pigmentado obtenido de la cabeza de camarón

    Directory of Open Access Journals (Sweden)

    Núñez-Gastélum, J. A.

    2011-09-01

    Full Text Available In this work the proximal analysis, physicochemical characterization, fatty acid profile and astaxanthin content of pigmented oil obtained by fermentation shrimp heads are presented. Lipids are the major components in the oil (95%. The saponification number is 178.62 mg KOH/g, iodine value 139.8 cg iodine/g, and the peroxide value was not detected. Density and viscosity were 0.92 mg/ml and 64 centipoises, respectively. The highest contents of fatty acids were linoleic (C18:2n6, oleic (C18:1n9 and palmitic (C16:0. Eicosapentaenoic acid (C20:5n3, EPA and docosahexaenoic acid (C22:6n3, DHA account for 9% of the total. The content of astaxanthin was 2.72 mg/g dry weight. The pigmented oil is a dietary source of nutrients with high value such as astaxanthin.

    En este trabajo se presenta el análisis proximal, caracterización físico-química, perfil de ácidos grasos y contenido de astaxantina en aceite pigmentado aislado por fermentación láctica de los residuos de camarón. Los lípidos son los componentes mayoritarios (95%. El índice de saponificación es 178.62 mg KOH/g, el de yodo 139.8 cg yodo/g, y los peróxidos no fueron detectados. La densidad y la viscosidad fueron de 0.92 mg/ml y 64 centipoises, respectivamente. Los ácidos grasos en mayor cantidad fueron el linoleico (C18:2n6, oleico (C18:1n9 y palmítico (C16:0. El ácido eicosapentaenoico (C20:5n3, EPA y el docosahexaenoico (C22:6n3, DHA suman el 9% del total. El contenido promedio de astaxantina fue de 2.72 mg/g base seca. El aceite pigmentado es una fuente dietética de nutrientes con alto valor como la astaxantina.

  2. Vitamina C, carotenoides, fenólicos totais e atividade antioxidante de goiaba, manga e mamão procedentes da Ceasa do Estado de Minas Gerais = Vitamin C, carotenoids, phenolic compounds and antioxidant activity of guava, mango and papaya from Ceasa of Minas Gerais State

    Directory of Open Access Journals (Sweden)

    Daniela da Silva Oliveira

    2011-01-01

    Full Text Available Este estudo teve como objetivo analisar o conteudo de compostos antioxidantes (acido ascorbico - AA, acido desidroascorbico - ADA, vitamina C total, licopeno, £]-caroteno, £]-criptoxantina e estimativa de compostos fenolicos e avaliar a atividade antioxidante, em goiaba, manga e mamao. A analise de carotenoides e vitamina C foi realizada por Cromatografia Liquida de Alta Eficiencia (CLAE. O teor de fenolicos totais foi determinado utilizando o reagente de Folin-Ciocalteu e leitura espectrofotometrica. A atividade antioxidante foi avaliada pelo Teste do 2,2-diphenil-2-picril-hidrazil (DPPH¡E e do Poder Redutor. A Anova (ƒÑ = 0,05 foi utilizada para a analise dos dados. Os teores dos constituintes antioxidantes diferiram entre as tres frutas, mas a goiaba foi a fruta que apresentou teores mais elevados de compostos fenolicos, vitamina C total, ADA e licopeno, alem dos maiores valores para atividade antioxidante. Foi constatada forte correlacao entre os testes que avaliaram a atividade antioxidante e o teor de fenolicos totais, demonstrando serem estes os principais compostos antioxidantes a contribuirem para a atividade antioxidante das frutas analisadas, em ambos os testes. E importante incentivar a utilizacao das frutas avaliadas neste estudo, tanto em nivel domestico quanto em estabelecimentos de alimentacao coletiva para aumentar o consumo de antioxidantes naturais pela populacao.This study aimed to analyze the content of antioxidant compounds (ascorbic acid - AA, dehydroascorbic acid - DHA, total vitamin C, lycopene, £]-carotene, £]-cryptoxanthin and phenolic compounds and to evaluate the antioxidant activity in guava, mango and papaya.The analysis of carotenoids and vitamin C was performed by high performance liquid chromatography (HPLC. The content of phenolic compounds was determined using the Folin-Ciocalteu reagent and spectrophotometric reading. Antioxidant activity was evaluated by testing the 2.2-diphenyl-2-picryl

  3. Removing volatile fatty acids during the anaerobic treatment of pig sewage; Remocion de acidos grasos volatiles durante el tratamiento anaerobio de aguas residuales porcicolas

    Energy Technology Data Exchange (ETDEWEB)

    Vazquez Borges, E.; Mendez Novelo, R.; Magana Pietra, A.; Partinez Pereda, P.; Fernandez Villagomez, G. [Facultad de Ingenieria, Universidad Atuonoma de Yucatan. Mexico (Mexico)

    1997-06-01

    This study examined the behaviour of a hybrid anaerobic digester in treating pig farm sewage. The experimental model consisted of a 208-litre UASB reactor at the bottom and a 195-litre high-rate sedimentator at the top. The digester was installed on a pig farm and its efficiency in removing volatile (acetic and propionic) fatty acids (VFA) was determined with hydraulic retention time (HTR) as the critical parameter for evaluating the anaerobic system`s performance. The results obtained with the five different HRTs used during the experiment are reported. The highest removal rates were obtained with an HRT of 2.8 days: 98% in the UASB, 28% in the sedimentator and 98% in the digester as a whole. An HRT of 1 day gave VFA removal rates of 40%, 12% and 50% in the UASB reactor sedimentator and digest respectively. (Author) 16 refs.

  4. Study of the desphosphatization process. The impact of nitrilotriacetic acid trisodium salt (NTA). Estudio del proceso de defosfatacion. Impacto de la sal tisodica del acido nitrilotriacetico SNTA

    Energy Technology Data Exchange (ETDEWEB)

    Peisajovich, A.; El Falaki, K.; Martin, G.

    1993-07-01

    In this paper we examined the effects of NTA on the removal of phosphorus process. The biological phosphorus removal process has been studied in the batch tests. This process indicated that there is a perturbation level (DD) of the phosphorus removal which is situated at 40 mg/g MVLSS. A dynamic lab-scale study showed a reduction of the nitrogen and phosphorus removal efficiency, ten days after the introduction of NTA in the influent water in concentration lower than DD. The precipitation of phosphate from wastewater has been examined using the Jar-Test method. The resultants of phosphate precipitation employed iron (III), aluminium (III) or lime did not reveal any differences in the efficiency between the coagulants. The present of NTA did not show a reduction of efficiency in the removal phosphorus process. (Author) 32 refs.

  5. Disolución anodica del niquel en medio acido. Caracterización de probetas termoplasticas conductroras del tipo grafito/niquel-polipropileno.

    OpenAIRE

    Negrete Barrera, Faustino Antonio

    2009-01-01

    RESUMEN El objetivo científico de este trabajo se centra principalmente en la caracterización de un tipo composite basado en la dispersión aleatoria de polvos micrométricos de grafito y níquel en el interior de una matriz termoplástica de polipropileno. Se han utilizado también técnicas microscópicas convencionales, de SEM, EDAX y STM para analizar la morfología superficial de las probetas. El composite estudiado es un material de tipo híbrido elaborado con polvos de níquel y de grafito gr...

  6. Radioprotection potential of the ascorbic acid on the carrageenan used as food additive; Potencial radioprotetor do acido ascorbico sobre a carragenana utilizada como aditivo alimenticio

    Energy Technology Data Exchange (ETDEWEB)

    Aliste, Antonio Joao; Mastro, Nelida Lucia del [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)]. E-mail: ajaliste@net.ipen.br; nlmastro@net.ipen.br

    2002-07-01

    Carrageenans are a group of natural carbohydrates that are present in the structure of certain varieties of red algae (Rhodophyceae). They are used in emulsions, for syneresis control and to grow up, to promote adhesiveness and dispersion. In the industry of foods they can be used for instance, as thickness and gelling agents, alone or together with other additives. The processing of foods by radiation is increasing considerably, because the efficiency of the process in the industrial decontamination of products. The objective of this work was to study the action of the ascorbic acid as potential radioprotector of the carrageenan against {sup 60} Co gamma radiation effects, using the viscosity as parameter. Samples of commercial carrageenan dissolved at 1,0% were irradiated in the presence or not of ascorbic acid, with doses of 0.0 kGy; 1.0 kGy; 2.5 kGy; 5.0 kGy and 10.0 kGy. After the irradiation the relationships viscosity/dose were established for the temperature of 60 deg C. For the dose of 10.0 kGy a better protecting effect of the ascorbic acid on the carrageenan was seen. The implications of the use of this antioxidant is discussed as a form of minimizing the effect of the radiation in irradiated foods. (author)

  7. The importance of the fatty acids in breast milk and in lacteal formulae; La importancia de los acidos grasos en la leche materna y en las formulas lacteas

    Energy Technology Data Exchange (ETDEWEB)

    Vega, S.; Gutierrez, R.; Radilla, M.; Ramirez, A.; Perez, J. J.; Schettino, B.; Ramirez, M. L.; Ortiz, R.; Fontecha, J.

    2012-11-01

    The best choice for feeding the newborn child is breast milk, and when it is not possible to breast feed a baby, the breast milk is replaced by lacteal formula, which is why numerous studies focus their attention on the analysis of the diverse components of baby formula as well as functions in the breast fed baby. Fatty acids are components of great nutritional importance in the fetus and in the newborn child. Nowadays it is estimated that the fetus, during the last trimester of the gestational period and the newborn child, during the first six months of life, need a great contribution of araquidonic and docosahexaenoic acids, due to the fact that the speed of transformation of the predecessors to the hepatic level are not sufficient to cover the metabolic requirements of these fatty acids and it is the mother who contributes them through placental transport during gestation and her milk during lactation. The Organization of Food and Agriculture (FAO), the World Health Organization (WHO), the European Society of Gastroenterology, Hepato logy and Pediatric Nutrition (ESPGHAN) and the American Academy of Pediatrics recommend that lacteal formula imitate the composition of the breast milk, especially in the fatty polyunsaturated acids (PUFA). (Author) 61 refs.

  8. Analytical methods for 2,4-D (Dichlorophenoxyacetic acid) determination; Metodos analiticos para la determinacion del 2,4-D (Acido diclorofenoxiacetico)

    Energy Technology Data Exchange (ETDEWEB)

    Martinez G, M.S.M

    1999-06-01

    The 2,4-D herbicide is one of the main pesticides for controlling the bad grass in crops such as the water undergrowth. In Mexico the allowed bound of this pesticide is 0.05 mg/l in water of 2,4-D so it is required to have methods trusts and exacts, which can used in order to detected low concentration of it. In this work we show some for the conventional techniques and for establishing the 2,4-D concentrations. The UV-Vis spectrometer and liquids chromatography due that they are the most common used nowadays. Beside, we introduce a now developed technique, which is based on the neutronic activation analysis. Though use of the UV-Vis spectrometer technique it was possible target the concentrations interval between 1 and 200 mg/l. In the liquids chromatography interval was between 0.1 and 0.9, and by the neutronic activation analysis the interval was between 0.01 and 200 mg/l. (Author)

  9. Natural Radiation in byproducts of the production of phosphoric acid; Radiacao natural em residuos gerados no processo de producao de acido fosforico

    Energy Technology Data Exchange (ETDEWEB)

    Silveira, Marcilei A. Guazzelli da; Cardoso, L.L., E-mail: marcilei@fei.edu.br [Centro Universitario da FEI, Sao Bernardo do Campo, SP (Brazil); Medina, N.H. [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Institutlo de Fisica

    2014-07-01

    Natural radiation is the largest source of radiation exposure to which man is subject. It is formed basically by cosmic radiation and the radionuclides present in the Earth crust, as {sup 40}K and the elements of the decay series of {sup 232}Th and {sup 238}U. Phosphate ores, which constitutes the raw material for the production of phosphoric acid, have a high rate of natural radiation from the decay series of {sup 232}Th and {sup 238}U. Phosphogypsum, which is naturally radioactivity, is a by-product of the production of phosphoric acid by the wet method. For each ton of phosphoric acid it is produced about 4.5 tons of phosphogypsum. This work presents the analysis of samples collected in all stages of the manufacturing process of phosphoric acid, which generates the phosphogypsum. Gamma-ray spectrometry was used to measure the concentration of the elements of the decay series of {sup 232}Th and {sup 238}U. All analyzed samples showed a high concentration of radionuclides, promoting the need for further steps in the process in order to reduce the presence of such radionuclides in the phosphogypsum. The results indicate the radionuclide {sup 238}U has higher contribution in some samples of the intermediate stages of the process. All samples exceeded the international average range of human exposure to terrestrial gamma radiation, which is 0.3 to 1.0 mSv/year. (author)

  10. EFECTO DE LA ADICION DE ACIDOS GRASOS POLIINSATURADOS SOBRE LA DINAMICA FOLICULAR, TASA DE GESTACIÓN Y RESPUESTA OVÁRICA EN OVEJAS PELIBUEY

    OpenAIRE

    J. Herrera Camacho

    2003-01-01

    El objetivo general del presente trabajo fue evaluar el efecto de la adición de ácidos grasos poliinsaturados en la dieta sobre la dinámica folicular, la tasa de fertilidad, prolificidad y respuesta superovulatoría en ovejas Pelibuey. Se realizaron tres experimentos. El experimento uno, se realizó en la Facultad de Medicina Veterinaria y Zootecnia durante el período julio-septiembre del año 2000. Se utilizaron 30 ovejas Pelibuey de condición corporal similar con el objeto de evaluar el efecto...

  11. Comparative experimental study on municipal wastewater disinfection with ozone and peracetic acid; Indagine sperimentale comparata sulla disinfezione di acque reflue urbane con ozono e acido peracetico

    Energy Technology Data Exchange (ETDEWEB)

    Andreottola, G.; Bertola, P.; Ziglio, G. [Trento Univ. (Italy). Dip. di Ingegneria Civile Ambientale

    1996-04-01

    The results of a pilot experimental study on the disinfection of treated municipal wastewater to be used in agriculture are presented. A comparative evaluation has been carried out on two parallel pilot-scale disinfection plants using respectively ozone and peracetic acid. After a preliminary sand filtration pilot unit. Both processes showed the capability of meeting the Italian standards for agricultural reuse, but the disinfection process with ozone required much higher doses than the one with peracetic acid, probably because of the higher reactivity of ozone, if compared to peracetic acid with the organic matter present in wastewater. Further research is needed in order to evaluate the efficiency of peracetic acid applied to microorganism different from the bacterial ones and to identify nature and consequences of possible disinfection by products.

  12. El acido esteárico: un posible sustituto para los ácidos grasos trans de origen industrial

    OpenAIRE

    Tavella, Marcelo; Delplanque, Bernadette; Valenzuela, Alfonso

    2011-01-01

    Trans isomers, contained in partially hydrogenated oils, which are used in the food industry, have been questioned and nowadays trends are heading towards reducing their consumption. The food industry is facing a dilemma, since in order to remove trans fatty acids, hydrogenated fats should be eliminated and replaced by fats rich in saturated fatty acids. Scientific research has shown that saturated fatty acids have negative effects on the lipid profile and its consumption is associated with a...

  13. Kraft pulp bleaching with molybdenum activated acid peroxide (P{sub Mo} stage); Branqueamento de polpa celulosica kraft de eucalipto com peroxido acido ativado por molibdenio

    Energy Technology Data Exchange (ETDEWEB)

    Rabelo, Marcos Sousa [Servico Nacional de Aprendizagem Industrial (SENAI), Lauro de Freitas, BA (Brazil). Dept. Regional da Bahia; Silva, Vanessa Lopes; Barros, Denise Pires de; Colodette, Jorge Luiz [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Engenharia Florestal; Sacon, Vera Maria; Silva, Marcelo Rodrigues da [Votorantim Celulose e Papel, Jacarei, SP (Brazil)

    2009-07-01

    Optimum conditions to run the P{sub Mo} stage for bleaching eucalyptus kraft pulp were 90 deg C, pH 3.5, 2 h, 0.1 kg/t Mo and 5 kg/t H{sub 2}O{sub 2}. The P{sub Mo} stage efficiency increased with decreasing pH (1.5-5.5) and increasing temperature (75-90 deg C), time (2-4 h), and hydrogen peroxide (3-10 kg/t) and molybdenum concentration (0.1-0.4 kg/t). The implementation of the P{sub Mo} stage, as replacement for the A stage, decreased total active chlorine demand of the OAZDP sequence by 6 kg/t to reach 90% ISO, both in laboratory and mill scale. Such practice resulted in decreased bleaching chemical costs to produce fully bleached pulp of 90% ISO. (author)

  14. Mechanical properties of poly(N-isopropylacrylamide-Co-itaconic acid) hydrogels; Propiedades mecanicas de hidrogeles de poli(N-sopropilacrilamida-Co-acido itaconico)

    Energy Technology Data Exchange (ETDEWEB)

    Valderruten, N. E.; Quintana, J. R.; Katime, I.

    2001-07-01

    It is well known that polymers of N-isopropylacrylamide (NIPA) show lower critical solution temperature (LCST) behavior in water and its gels have a volume phase transition at about 34 degree centigree in water. In this study, we reported the polymerization of NIPA in the presence of N,N methylenebisacrylamide (BIS). Poly(N-isopropylacrylamide) and poly(N-isopropylacrylamide-co-itaconic acid) hydrogels were obtained by swelling the resultant solid xerogels to equilibrium in water. The effects of monomer composition and concentration of added cross-linking agent on the swelling behavior and mechanical properties of these hydrogels at 22 and 37 degree centigree were investigated, the latter involving measurements of shear in a DMTA system. The storage moduli at 22 degree centigree lay within the range 9.08-5.08 KPa. At a fixed BIS concentration, an increase from 22 to 37 degree centigree resulted in an increase in the shear moduli and the effective crosslinking density (v{sub e}) and a decrease in the interaction parameter hydrogel/water, {chi}. (Author) 6 refs.

  15. Influence of state of charge in lead-acid batteries operating in PV systems; Comportamiento no repetitivo de las baterias de plomo-acido operando en sistemas FV.

    Energy Technology Data Exchange (ETDEWEB)

    Vela, N.; Chenlo, F.

    2004-07-01

    Correct determination of the overcharge cut-off voltage is a key point for both the optimal operation and maximum life-time of batteries in photovoltaic (PV) systems. This work presents the results of analysing the influence on charge voltage of different operation conditions, mainly current rate, temperature and state of charge (SOC). From the results obtained we have observed that voltage evolution during a charge process depends on its activation degree of the battery. The battery activation is reached when battery was previously fully charged. So, we can conclude that variation of the charge voltage with time as function of starting point (fully charged or fully discharged) together with current rate and temperature should be taking into account in the battery SOC determination and in the design of charge controllers. (Author)

  16. Modelado Cinético de la Disolución de Hidroboracita en Acido Clorhídrico Kinetics Modeling of Hydroboracite Dissolution in Hydrochloric Acid

    OpenAIRE

    Graciela V Morales; Oscar D Quiroga; Ebelia N Tolaba

    2010-01-01

    Se realizó el modelado cinético de la disolución de hidroboracita en soluciones de ácido clorhídrico. La disolución se llevó a cabo en un reactor tanque agitado discontinuo a presión atmosférica. Se investigó el efecto de la temperatura, el tamaño de partícula, la relación sólido/líquido, y la concentración del ácido sobre la velocidad de disolución del mineral. Se observa que la velocidad de disolución es muy rápida en todos los casos estudiados, aumenta con el incremento de la temperatura y...

  17. Polyesters production from the mixture of phthalic acid, terephthalic and glycerol; Producao de poliesteres a partir da mistura de acido ftalico, tereftalico e glicerol

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, A.L.S.; Oliveira, J.C.; Miranda, C.S.; Boaventura, J.S.; Jose, N.M., E-mail: adrianaequfba@gmail.co [Universidade Federal da Bahia (GECIM/UFBA), Salvador, BA (Brazil). Inst. de Quimica. Grupo de Energia e Ciencias dos Materiais; Carvalho, R.F. [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Escola Politecnica. Curso de Mestrado em Engenharia Ambiental Urbana

    2010-07-01

    Glycerin, a byproduct of biodiesel is currently an environmental and economic problem for producers of this renewable fuel in Brazil and in others parts of the world. In order to offer new proposals for recovery, it is used for the manufacture of polyesters used in applications in diverse areas such as construction and automobile industry. This work reports the production of polymer from the mixture of terephthalic and phthalic acid in three different proportions. The polyesters showed good thermal stability, analyzed by TGA and DSC, with an increase proportional to the terephthalic acid content. The X-ray diffraction patterns show that the samples are semi crystalline polymers. The micrographs indicated the presence of a smoother surface in the polyester that has a larger amount of phthalic acid, as reported in the literature. Therefore, the materials showed good thermal properties and morphological characteristics, so it consists in a new alternative to use glycerin. (author)

  18. Determinacion de la configuracion E-Z de los acidos Fumarico y Maleico. Un experimento orientado a incentivar el desarrollo de la investigacion cientifica en alumnos de Pregrado

    Directory of Open Access Journals (Sweden)

    Bustos Carlos

    2000-01-01

    Full Text Available In this work we intend to eliminate the idea that laboratory exercises seem like cookbooks. That is, exercises shall be presented as a problematic situation. Based on observation and experimentation, the students should determine the E-Z configuration of maleic and fumaric acids. The basis of this laboratory exercise is the acid-catalyzed isomerization of maleic acid to fumaric acid. Students are given the starting material, reagents and the experimental procedure. They are told that the starting material is a dicarboxylic acid containing a C=C double bond of formula C4H4O4. Students determine melting points, solubilities, acidity and chromatographic patterns for both the starting material and the product, so that a configuration of each acid can be proposed. This type of experiment yields excellent results, because the students are left to deduce that maleic acid is less stable than fumaric acid. Additionally, they conclude that maleic acid is the "Z" isomer and fumaric acid is the "E" isomer. Finally, this laboratory exercise allows the students to develop simultaneously their critical-thinking skills with the respective laboratory techniques and not to see chemistry as recipes to be followed.

  19. Behaviour and reliability of lead-acid batteries in stand-alone photovoltaic systems; Comportamiento y durabilidad de baterias de plomo-acido en sistemas fotovoltaicos autonomos

    Energy Technology Data Exchange (ETDEWEB)

    Vela Barrionuevo, N.

    2007-07-01

    Vented stationary lead-acid batteries are currently the most commonly used type of accumulator in stand-alone PV systems. The state-of the art of the technology suggests that lead-acid batteries will maintain a predominant position in the PV market for the next years. Additionally to the specific operating requirements of batteries in PV systems, there are other questions not completely solved related to battery characterisation and testing methods for this type of application. The objective of this work is to contribute to the operational optimisation and reliability improvement of lead-acid batteries operating in stand-alone PV systems. This objective has been approached by means of eminently experimental works. These works are focused firstly on functional characterisation of batteries, with special emphasis on capacity and characteristic voltages study and secondly on degradation analysis of these batteries taking into account the specific working conditions of stand-alone PV systems which main characteristic is its wide variability of operational conditions (current rates, temperature and state of charge). In relation with battery characterisation, a procedure for the determination of the usable capacity of lead-acid batteries has been established and applied to a set of commercial batteries at different current rate and temperature conditions. From each battery experimental data and using a model equation describing the battery capacity as function of current rate and temperature, the corresponding parameters for each battery have been obtained. A comparative analysis of the model application to experimental data and data supplied by the manufacturer is included. Also, initial gassing voltage and end-of-charge voltage values have been measured at different current rate and temperature conditions for the whole set of batteries. Experimental values of voltage from each battery have been fitted with high degree of accuracy to simple mathematical expressions for both characteristic voltages as function of current rate and temperature. From the regression analysis, temperature coefficients have been obtained. To conclude the characterisation works, an study of charge voltage evolution and overall charge discharge efficiency at different operating conditions (current rate and temperature) combined with different initial test conditions and variations of state of- charge has been performed. It has been observed that battery voltage, in many cases, could not be indicative of its actual state of charge, instead of this a strong dependence on the voltage evolution on initial battery condition has been obtained. With respect to battery reliability, an analysis of failures and degradation mechanisms of lead-acid batteries under specific PV operation condition is presented. From this analysis we can conclude that the main causes of degradation of lead-acid batteries in PV systems are due to extended periods working at overcharge or over discharge conditions and typical cycling condition. Because of this, different accelerated battery tests have been developed to reproduce these degradation mechanisms. The objective of these procedures is to considerably reduce the tests duration without causing any alteration to the physical meaning of the actual degradation mechanism. Results of applying these accelerated degradation tests to two different batteries are presented. Finally, in basis of the obtained results, a general qualification test sequence for lead-acid batteries in PV stand-alone systems has been proposed. The proposed sequence includes individual specific test procedures for: technical specifications included in the documentation supplied with the battery, visual inspection, characterisation (full charge, capacity and efficiency) and reliability (overcharge, over discharge and cycling). (Author)

  20. Effect of the temperature and oxalic acid in the uranyl sorption in zircon; Efecto de la temperatura y acido oxalico en la sorcion de uranilo en circon

    Energy Technology Data Exchange (ETDEWEB)

    Ordonez R, E.; Almazan T, M. G.; Garcia G, N. [ININ, Departamento de Quimica, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico); Hernandez O, R., E-mail: eduardo.ordonez@inin.gob.mx [Instituto Tecnologico de Veracruz, Ingenieria Quimica, Miguel Angel de Quevedo No. 2779, 91860 Veracruz (Mexico)

    2012-10-15

    In this work the results of the temperature effect study are presented on uranyl solutions adsorbed on zirconium silicate (ZrSiO{sub 4}) and also on the compounds formed in surface with oxalic acid. The adsorption isotherms of uranyl on hydrated zircon with NaClO{sub 4} 0.5 M, show an increase of the uranyl sorption efficiency when increasing the temperature from 20 to 4 C with a sudden descent in this efficiency when changing the temperature at 60 C. The uranyl sorption efficiency increases to hydrate the zircon with a solution of oxalic acid 0.1 M, maintaining the same tendency regarding to the temperatures of the sorption in medium NaClO{sub 4} 0.5 M. The complex formation in the zircon surface with organic acids of low molecular weight increases the fixation of the uranyl in solution due to the formation of ternary systems, in the order Zircon/A. Organic/Uranyl, without altering their response to the temperature. (Author)

  1. Acidos húmicos y fúlvicos en la producción hidropónica de Chile Manzano (Capsicum pubescens R y P) en invernadero.

    OpenAIRE

    Hernández Hernández, Antelma

    2011-01-01

    Para determinar el efecto de substancias húmicas (SH) en el crecimiento del chile manzano (Capsicum pubescens R y P), en invernadero, se evaluaron tres niveles de presión osmótica de solución nutritiva Steiner (0.54, 0.72 y 0.90 atm) y tres concentraciones de aplicación foliar de substancias húmicas (0.5, 1, 1.5 ml.L-1 de agua de las SH). Las variables medidas fueron: diámetro ecuatorial (DE), diámetro polar (DP), volumen (VOL), grosor de pericarpio (GP), numero de frutos comerciales (NFRC), ...

  2. Acido-basic control of the thermoelectric properties of poly(3,4-ethylenedioxythiophene)tosylate (PEDOT-Tos) thin films

    DEFF Research Database (Denmark)

    Khan, Zia Ullah; Bubnova, Olga; Jafari, Mohammad Javad;

    2015-01-01

    in the nanoscale morphology due to anion exchange between tosylate and Cl- or OH-. But, we identified that changing the pH leads to a tuning of the oxidation level of the polymer, which can explain the changes in thermoelectric properties. Hence, a simple acid-base treatment allows finding the optimum...

  3. Evolution of biochemical parameters in irradiated fishes: Serum proteins and intestinal nucleic acids; Evolucion de parametros bioquimicos en peces irradiados: Proteinas en suero y acidos nucleicos en intestino

    Energy Technology Data Exchange (ETDEWEB)

    Garces, F.; Andres, P.; Davila, C. A.

    1976-07-01

    In sublethal gamma-irradiated C. auratus, a sudden decrease of total serum protein concentration and a preferential descent of the low molecular weight gamma-globulin fraction have been observed. These effects are transient and after different latent periods dependent on doses, normal values are recovered, A temporal failure of a vascular permeability regulation system is probably implied. The DMA depolymerization. observed in the intestine indicates the action of radio-induced DNA degradation mechanisms since this effect is independent on doses. (Author) 29 refs.

  4. Degradation of polylactic acid (Pla) at different doses of gamma radiation; Degradacion del acido polilactico (PLA) a diferentes dosis de radiacion gamma

    Energy Technology Data Exchange (ETDEWEB)

    Castillo R, Y.

    2015-07-01

    The excessive use of polymers such as polyethylene (PET), polystyrene (Ps) and recently the polylactic acid (Pla) that take more than 20 years to degrade, have caused great pollution in the environment. In this study the effects of gamma radiation in the Pla to different doses were studied, in order to reduce the degradation time of this polymer. The changes in physico-chemical structure of Pla during radiation were studied by thermo-gravimetric/Mass analysis; differential scanning calorimetry; scanning electron microscopy; X-ray dispersive analysis; infrared spectroscopy; X-ray diffraction and mechanical tests of hardness, elasticity and deformation. With scanning electron microscopy, the morphology of the Pla surface unirradiated was observed, in which an apparently smooth surface was observed, after changes that had the Pla when irradiated also was observed, where the effects of radiation were observed in form of scratch, agglomeration and small fractures. By X-ray dispersive analysis was determined and verified the elemental chemical composition of the Pla; as expected the tests showed only carbon, oxygen and hydrogen. With thermo-gravimetric/Mass analysis the decomposition temperatures of Pla were determined, identifying that the degradation compounds are CO, CO{sub 2} and CH{sub 4}. With infrared spectrometry the major peaks of Pla were observed before and after being irradiated with increasing of radiation dose the intensity of the bands decreased. Also by X-ray diffraction was observed that the polymer is an amorphous material. The mechanical tests indicate that the values of each of the tests decrease significantly with increasing the radiation dose. (Author)

  5. Myrsinoic A acid and its derivative: in vitro inhibitors of photosynthesis; Acido myrsinoico A e derivado: inibidores da fotossintese in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Burger, Marcela Carmen de M.; Oliveira, Gracielle S. de; Menezes, Antonio Carlos S. [Universidade Estadual de Goias, Anapolis, GO (Brazil). Unidade Universitaria de Ciencias Exatas e Tecnologicas; Vieira, Paulo Cezar; Silva, Maria Fatima das G.F. da [Universidade Federal de Sao Carlos (UFSCar), SP (Brazil). Dept. de Quimica; Veiga, Thiago A.M., E-mail: tveiga@unifesp.br [Universidade Federal de Sao Paulo (UNIFESP), SP (Brazil). Inst. de Ciencias Ambientais, Quimicas e Farmaceuticas. Dept. de Ciencias Exatas e da Terra

    2012-07-01

    Myrsinoic A acid, isolated from Myrsine cuneifolia and its hydrogenated derivative had their effect on photosynthesis tested. The compounds inhibited the electron flow (basal, phosphorylating and uncoupled) from water to methyl viologen; therefore, they act as Hill reaction inhibitors in spinach thylakoids. They inhibited partial reactions of PSII electron flow from water to 2,5-dichloro-1,4-benzoquinone, from water to sodium silicomolybdate, and partially electron flow from diphenylcarbazide to 2,6-dichloroindophenol. Their inhibition sites were at the donor and acceptor sides of PSII, between P{sub 680} and Q{sub A}. Chlorophyll {alpha} fluorescence measurements confirmed the behavior of the compounds (pool of quinones). (author)

  6. Behaviour of the 1-Ascorbic as supporting Electrolyte. Influence of the Magnesium Ion; Comportamiento del acido 1-Ascorbico como electrolito soporte influencai del ion magnesio

    Energy Technology Data Exchange (ETDEWEB)

    Alonso Lopez, J.

    1962-07-01

    The behaviour of 1-ascorbic acid, as supporting electrolyte of the uranyl ion in a 0{sub 1}-0.7 M concentration range, and the influence of pH on the diffusion current and half wave potential of 0,1 M uranyl ion is studied. The cathodic waves from 0 to -2,5 volts, with mercury dropping electrode are studied in fresh 0,1 M aqueous solution in presence of Mg{sup 2}+ and at 2,0-12 pH range. A kinetic current with a half wave potential of 0,85 v. vs. Hg. b.e. is obtained at pH> 9,5 appears a tilth wave a -0,60 v. The pH variation does not influence these potentials. (Author) 18 refs.

  7. Spectrophotometric determination of titanium with ascorbic acid in aluminium alloys and other materials. Determinacion espectrofotometrica de titanio con acido ascorbico en aleaciones de base aluminio y otros materiales

    Energy Technology Data Exchange (ETDEWEB)

    Bosch Serrat, F. (Departamento de Quimica Analitica. Facultad de Qauimica. Valencia (Spain))

    1994-01-01

    A spectrophotometric determination of titanium with ascorbic acid in aluminium alloys and bauxite is described. The proposed procedures permit to determine levels of titanium down to 5.10 ''3% with a good accuracy and precision. (Author) 13 refs.

  8. PRODUCCIÓN DE ACIDO CLAVULÁNICO POR FERMENTACIÓN DE Streptomyces clavuligerus: EVALUACIÓN DE DIFERENTES MEDIOS DE CULTIVO Y MODELADO MATEMÁTICO

    Directory of Open Access Journals (Sweden)

    CLAUDIA PATRICIA SANCHEZ HENAO

    2012-01-01

    Full Text Available El ácido clavulánico (AC es un antibiótico b-lactámico con una potente capacidad inhibidora de la actividad b-lactamasa. En el presente trabajo se evaluaron cuatro medios de cultivo reportados en la bibliografía y uno propuesto por los autores, para la producción de AC. Se evaluó también la concentración de glicerol adecuada para el medio seleccionado y con base en los resultados obtenidos se realizó una evaluación de la producción de AC en un biorreactor de laboratorio. Se propuso un modelo matemático y se evaluó su ajuste a los resultados experimentales. Se observó que los medios complejos con presencia de una mezcla de aminoácidos libres, son adecuados para la producción de AC. El glicerol presentó un valor óptimo frente a la producción de AC en 50 g.L-1 y se observó el efecto inhibitorio a concentraciones de 100 g.L-1. Con el medio complejo y 50 g.L-1 de glicerol, la concentración de AC alcanzada fue de 994 mg.L-1. El modelo matemático propuesto que incluye una cinética tipo Contois para la biomasa y una cinética de formación de producto parcialmente asociado al crecimiento, presentan un ajuste significativo con un 95% de nivel de confianza.

  9. Sprayed microspheres of poly(lactic acid) obtained with calcium compounds; Microesferas aspergidas de poli(acido latico) obtidos com compostos de calcio

    Energy Technology Data Exchange (ETDEWEB)

    Goncalves, Raquel P.; Picciani, Paulo H. de Souza; Dias, Marcos L., E-mail: raquelpires@ima.ufrj.br [Instituto de Macromoleculas - Universidade Federal do Rio de Janeiro - UFRJ, Rio de Janeiro, RJ (Brazil)

    2011-07-01

    In this work PLLA and PDLA were synthesized using calcium methoxide (Ca(OMe){sub 2}) as initiator. This compound shows good activity in the bulk polymerization of L-lactide (LLA) and D-lactide (DLA) producing polymers with average molecular weight up to 22,300 g/mol, but with microstructure containing a significant amount of estereoerros, as revealed by {sup 13} C NMR. Block copolymers containing blocks of L-and D-lactic acid were also prepared, using the method of sequential addition of LLA and DLA in an attempt to obtain stereo complexes. Analyses of scanning electron microscopy (SEM) revealed that the polymers obtained with catalysts of calcium produced PLA microspheres with diameters of around 5 {mu}m via electro spray technique. (author)

  10. Elution of nickel ions from alginate beads in an acid media; Elucion de iones de niquel desde esferas de alginato en un medio acido

    Energy Technology Data Exchange (ETDEWEB)

    Aracena, A.; Carcamo, F.; Jerez, O.; Constanzo, R.

    2016-05-01

    Elution of nickel ions from alginate beads was studied in a temperature range of 2 to 80 degree centigrade and a sulfuric acid concentration between 0.005 to 2.5 g L{sup -}1. The elution mechanism was established by ion exchange between nickel and ions protons, obtaining a value of 93% elution at a temperature of 80 degree centigrade and a H{sub 2}SO{sub 4} concentration of 0.25 g L{sup -}1. The influence of other acids on nickel elution was also studied. The nickel elution rate was significantly influenced by temperature and concentration of H{sub 2}SO{sub 4}. Elution kinetics of nickel ions was studied, and the 1-(1-α){sup 1}/3=k{sub a}ppt model properly described the kinetics of this reaction. The dependence of nickel elution on the sulfuric acid concentration was of the order of 0.33. Moreover, the intrinsic rate constants were determined and an activation energy value of 54.5 kJ mol{sup -}1 was obtained for the temperature range studied. The results indicated that the nickel elution is a process controlled by chemical reactions. (Author)

  11. l aceite de atún como fuente de ácidos grasos ω-3 en el huevo de gallina

    Directory of Open Access Journals (Sweden)

    Vázquez-Valladolid, J. L.

    2005-06-01

    Full Text Available Fish oils are a source of polyunsaturated omega 3 fatty acids (AG ω3, mainly eicosapentaenoic acid (EPA and docosahexaenoic acid (DHA, which supply many benefits to human health. Tuna oil was used as a source of marine 3ω FA to enrich eggs by supplementing the diet of egg-laying hens with tuna oil. One hundred and twenty White Leghorn hens of 90 weeks old were allocated on three treatments with four replicates each, on a completely random design. Treatments consisted of adding 1 % and 2 % of tuna oil to the diets. The total lipids and ω3FA of the eggs were determined. The egg-laying hen dietary tuna oil supplement (1 and 2 % enriched eggs with 300 % more EPA (0.40, 1.37, 1.54 mg/g lipids and DHA (7.90, 24.67, 24.50 mg/g lipids versus the control egg . The ω6:ω3 ratio decreased ( 11.4:1, 3.8:1, 3.0:1 as dietary tuna oil increased. There were no differences (p>0.05 among treatments in productive performance and egg quality, except egg weight which decreased with the tuna oil.Los aceites de pescado son una fuente de lípidos poliinsaturados de origen animal, principalmente de los ácidos grasos omega-3 (AG ω-3, eicosapentaenoico (EPA y docosahexaenoico (DHA, beneficiosos para la salud. En este trabajo se utilizó el aceite de atún como fuente de estos nutrientes en el huevo para consumo. Se utilizaron 120 gallinas blancas Leghorn Isa-Babcock B-300 de 90 semanas de edad, distribuidas al azar en tres tratamientos, con cuatro réplicas cada uno. Los tratamientos consistieron en adicionar 1% y 2% de aceite de atún en la dieta de las gallinas ponedoras. Se determinaron los lípidos totales y AG ω-3 en el huevo, obteniéndose 300 % más de EPA (0,40, 1,37, 1,54 mg/g lípidos y DHA (7,90, 24,67, 24,50 mg/g lípidos al adicionar 1 y 2% de aceite de atún en la dieta de las aves, que en el grupo testigo. La relación ω-6: ω-3 en el huevo disminuyó con el suplemento dietético de aceite de atún (11,4:1, 3,8:1, 3:1, lo mismo ocurri

  12. Nutritional enrichment of vegetable oils with long-chain n-3 fatty acids through enzymatic interesterification with a new vegetable lipase

    Directory of Open Access Journals (Sweden)

    Sousa, J. S.

    2015-06-01

    diferentes fuentes mediante catálisis enzimática. Tras la reacción de interesterificación, que también fue catalizada por la VLP, el contenido de PUFA en el aceite de coco aumentó casi diez veces de 1,8% a 17,7%. En el aceite de palma, el contenido de PUFA aumentó dos veces desde 10,5% a 21,8%, mientras que en el aceite de oliva el nivel de PUFA incrementó de 8,6% a 21,3%. La mezcla de ácido eicosapentaenoico (EPA y ácido docosahexaenoico (DHA (3,7% a 3,9% fué incorporada a la fracción de triacilgliceroles de cada uno de los aceites, coco, palma y oliva. Mediante hidroesterificación (hidrólisis seguido de interesterificación todos los aceites vegetales interesterificados ensayados contienen en una cucharada suficientes niveles de EPA y DHA para cubrir los niveles recomendados de ingesta de humanos adultos.

  13. Resting heart rate in infants and toddlers: variations associated with early infant diet and the omega 3 fatty acid DHA

    Science.gov (United States)

    Although early postnatal nutrition can have long-term effects on developmental processes, the influence of infant diet on the maturation of cardiac development has not been documented. To study this relationship we recorded resting heart-rate (HR) in awake, healthy infants and toddlers exclusively b...

  14. Enhancement of Anti-Dermatitis Potential of Clobetasol Propionate by DHA [Docosahexaenoic Acid] Rich Algal Oil Nanoemulsion Gel

    Science.gov (United States)

    Sarfaraz Alam, Mohammad; Ali, Mohammad Sajid; Zakir, Foziyah; Alam, Nawazish; Intakhab Alam, Mohammad; Ahmad, Faruque; Siddiqui, Masoom Raza; Ali, Mohammad Daud; Ansari, Mohammad Salahuddin; Ahmad, Sarfaraz; Ali, Maksood

    2016-01-01

    The aim of the present study was to investigate the potential of nanoemulsion formulation for topical delivery of Clobetasol propionate (CP) using algal oil (containing omega-3 fatty acids) as the oil phase. CP has anti-inflammatory, immunomodulatory and antiproliferative activities. However, its clinical use is restricted to some extent due to its poor permeability across the skin. Algal oil was used as the oil phase and was also exploited for its anti-inflammatory effect along with CP in the treatment of inflammation associated with dermatitis. Nanoemulsion formulations were prepared by aqueous phase titration method, using algal oil, tween 20, PEG 200 and water as the oil phase, surfactant, co-surfactant and aqueous phase respectively. Furthermore, different formulations were subjected to evaluate for ex-vivo permeation and in-vivo anti-inflammatory, irritation and contact dermatitis studies. The optimized nanoemulsion was converted into hydrogel-thickened nanoemulsion system (HTN) using carbopol 971 and had a viscosity of 97.57 ± 0.04 PaS. The optimized formulation had small average diameter (120 nm) with zeta potential of -37.01 mV which indicated good long-term stability. In-vivo anti-inflammatory activity indicated 84.55% and 41.04% inhibition of inflammation for drug loaded and placebo formulations respectively. The assessment of skin permeation was done by DSC and histopathology studies which indicated changes in the structure of epidermal membrane of skin. Contact dermatitis reveals that the higher NTPDase activity in the treatment with the CP-loaded nanoemulsion could be related to the higher anti-inflammatory effect in comparison with placebo nanoemulsion gel. PMID:27610146

  15. Research Pogress of EPA and DHA from Aatic%水产品中EPA和DHA的研究进展

    Institute of Scientific and Technical Information of China (English)

    郭玉华; 李钰金

    2011-01-01

    EPA和DHA是人体中重要的不饱和脂肪酸,具有许多重要的生理功能,对人类和动物的生长发育和健康起着重要的作用,本文简要介绍了EPA和DHA的生理功能,分离提取方法及水产来源的EPA和DHA研究现状,并对其发展前景进行了展望.

  16. DHA Serum Levels Were Significantly Higher in Celiac Disease Patients Compared to Healthy Controls and Were Unrelated to Depression

    NARCIS (Netherlands)

    Hees, van N.J.M.; Giltay, E.J.; Geleijnse, J.M.; Janssen, N.; Does, van der A.J.W.

    2014-01-01

    Objectives: Celiac disease (CD), a genetically predisposed intolerance for gluten, is associated with an increased risk of major depressive disorder (MDD). We investigated whether dietary intake and serum levels of the essential n-3 polyunsaturated fatty acids (PUFA) eicosapentaenoic acid (EPA) and

  17. 富集DHA、EPA的保健蛋和肉%Quail eggs and meat rich in DHA and EPA

    Institute of Scientific and Technical Information of China (English)

    郑婵颖; 郑海龙

    2001-01-01

    以添加质量浓度为50 kg/m 3的粉末化鱼油的饲料及一般饲料喂养小鹌鹑.2个月后,对所产的鹌鹑蛋和鹌鹑肉进行EPA和DHA测试.结果表明:全蛋黄中的EPA和DHA平均值,喂添加鱼油饲料的鹌鹑蛋远高于喂一般饲料的鹌鹑蛋;喂5 d添加鱼油饲料的鹌鹑蛋其EPA和DHA平均值可达49.06 mg;且该值将随着喂添加鱼油饲料天数的增加而增大.喂60 d添加鱼油饲料时,每只鹌鹑肉含EPA和DHA的平均值为喂60 d一般饲料的52倍.这种富含DHA的蛋和肉没有鱼油的特殊腥味,特别适合孕妇、婴儿食用,也适合于中、老年人作为保健品食用.

  18. Production of EPA and DHA by Marine Algae%利用海藻生产EPA和DHA

    Institute of Scientific and Technical Information of China (English)

    胡爱军; 丘泰球; 梁汉华

    2001-01-01

    EPA和DHA具有多种重要的生理功能,已引起食品、医药等领域的高度重视.利用海藻作为生产EPA和DHA的又一来源具有巨大的潜力和十分重要的意义.着重阐述了海藻的EPA、DHA的含量和生产优势,并叙述了EPA、DHA的生产方法.

  19. 高产EPA和DHA藻株的筛选%SELECTION OF ALGAL STRAINS OF HIGH PRODUCTION OF EPA AND DHA

    Institute of Scientific and Technical Information of China (English)

    张秋会; 马莺

    2003-01-01

    为筛选出高产 EPA和 DHA藻株,针对 8种海洋微藻进行培养,测定了它们的最佳吸收波长和生长曲线.藻体在对数期末期收获,经过提取脂肪,进行皂化酯化处理后用气相色谱测定 EPA和 DHA的含量.结果筛选出 EPA高产藻株为新月菱形藻, EPA产量为 16mg/L,占粗脂肪重的 26%,占细胞干重的 3.3%;等边金藻为 DHA高产藻株, DHA产量为 3.2mg/L,占粗脂肪重的 9.1%,占细胞干重的 5.2%.

  20. Optimized Analytical Method to Determine Gallic and Picric Acids in Pyrotechnic Samples by Using HPLC/UV (Reverse Phase); Optimizacion del Metodo Analitico mediante HPLC/UV Operando en Fase Inversa para la Determinacion de Acido Galico y Acido Picrico en Muestras de Origen Pirotecnico

    Energy Technology Data Exchange (ETDEWEB)

    Garcia Alonso, S.; Perez Pastor, R. M.

    2013-10-01

    A study on the optimization and development of a chromatographic method for the determination of gallic and picric acids in pyrotechnic samples is presented. In order to achieve this, both analytical conditions by HPLC with diode detection and extraction step of a selected sample were studied. (Author)

  1. Impregnation of 12-tungstophosphoric acid on silica - part II: effect of different solvents on the impregnation and catalytic activity in methyl esterification of stearic acid; Impregnacao do acido 12-tungstofosforico em silica - parte II: efeito de diferentes solventes na impregnacao e atividade catalitica na esterificacao metilica de acido estearico

    Energy Technology Data Exchange (ETDEWEB)

    Scroccaro, Karine Isabel; Yamamoto, Carlos I., E-mail: karineisabel@yahoo.com.br [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Centro Politecnico. Departamento de Engenharia Quimica; Tanobe, Valcineide O. de A.; Oliveira, Alan Antonio de [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Departamento de Engenharia e Tecnologia Florestal; Wypych, Fernando [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Departamento de Quimica

    2014-04-15

    Materials obtained by the immobilization of 12-tungstophosphoric acid (PTA) on silica using the method of impregnation with excess solution in distinct solvents (aqueous HCl, methanol:H{sub 2}O, and acetonitrile) were evaluated for use as catalysts in the methyl esterification of stearic acid. Optimum conditions were established for the impregnation of 0.5 g (w/w) of PTA on amorphous silica, under stirring at 150 rpm for 24 h, using 20 mL of 0.1 mol L{sup -1} HCl as the solvent. After calcination at 200 deg C, high conversions were obtained under mild reaction conditions, resulting in high turnover numbers. The catalyst was evaluated in ten catalytic cycles of use, where the activity was reduced only slightly, attesting its stability and the possibility to apply it to industrial production of methylesters. (author)

  2. Marginal microleakage in vitro study on class V cavities prepared with Er:YAG laser and etched with acid or etched with Er:YAG laser and acid; Estudo in vitro da microinfiltracao marginal em cavidades classe V preparadas com laser de Er:YAG e condicionadas com acido ou com laser de Er:YAG e acido

    Energy Technology Data Exchange (ETDEWEB)

    Tavares, Henrique Dutra Simoes

    2001-07-01

    Microleakage at the interface between the teeth and the restorative materials remains a problem with composite resin restorations. Microleakage at the gingival margins of class V cavities restorations still challenge as they are usually placed in dentin and/or cementum. Previous studies have shown that the cavity preparation with Er:YAG laser is possible. It has been reported that Er:YAG laser has ability to create irregular surface providing micromechanical retention for adhesive dental restorative materials and to improve marginal sealing. The purpose of this in vitro study was to evaluate the marginal microleakage on class V cavities prepared with Er:YAG laser and etched with acid or with Er:YAG laser and acid, in compared to those prepared and etched conventionally. Thirty human molars were divided into three groups, namely: group I - prepared with Er:YAG laser (KaVo KEY Laser II - Germany) and etched with 37% phosphoric acid; group II - prepared with Er:YAG laser and etched with Er:YAG laser and 37% phosphoric acid; group III (control group) - prepared with high speed drill and etched with 37% phosphoric acid. All cavities were treated with same adhesive system (Single Bond - 3M) and restored with the composite resin (Z100 - 3M), according to the manufacturer's instructions. The specimens were stored at 37 deg C in water for 24 hours, polished with Sof-Lex discs (3M), thermally stressed, sealed with a nail polish coating except for the area of the restoration and 1 mm around it, and immersed in a 50% aqueous solution of silver nitrate for 24 hours. After that, the specimens were rinsed in water, soaked in a photodeveloping solution and exposed to a fluorescent light for 8 hours. The teeth were embedded in an autopolymerizing resin and sectioned longitudinally using a diamond saw microtome under running water. The sections were photographed. The microleakage at the occlusal cavity and at the gingival margins of each specimen was evaluated with scores (0-3) by three calibrated examinators. Both results on occlusal and gingival margins have shown no statistic significant difference among the three groups evaluated (p>0.05). The Er:YAG laser have shown to be as effective as the conventional methods for cavity preparation and etching, suggesting its use for the class V cavity preparation and enamel etching. (author)

  3. Development of methodology for the synthesis of poly(lactic acid-co-glycolic acid) for use in the production of radioactive sources; Desenvolvimento da metodologia para sintese do poli(acido latico-co-acido glicolico) para utilizacao na producao de fontes radioativas

    Energy Technology Data Exchange (ETDEWEB)

    Peleias Junior, Fernando dos Santos

    2013-07-01

    According to World Health Organization (WHO), cancer is a leading cause of death worldwide. Prostate cancer is the second most common cancer in men. A method of radiotherapy which has been extensively used is brachytherapy, where radioactive seeds are placed inside the area requiring treatment. Iodine-125 seeds can be placed loose or stranded in bioabsorbable polymers. Stranded seeds show some advantages, since they reduce the rate of seed migration, an event that could affect the dosimetry of the prostate and cause unnecessary damage to healthy tissues or organs. For Iodine-125 stranded seeds, polyglactin 910 (poly(lactic-co-glycolic acid)) (PLGA), with a coverage of polyglactin 370 (Vicryl Registered-Sign ) is used. It was purposed in this dissertation, the study and development of the synthesis methodology for PLGA via ring-opening polymerization, as well as its characterization, with the objective of using the synthesized material to manufacture a material similar to RAPID Strand{sub Registered-Sign }. The results obtained show that it was possible to determine the optimal reaction parameters (time and temperature) for PLGA in 80/20 (lactide/glycolide) ratio. Using a temperature of 110 Degree-Sign C and reaction time of 24h, a yield of 86% was obtained, and increasing the reaction time to 72 hours, the yield was higher than 90%. The molecular mass values obtained from the samples are still very low compared to those obtained by other authors in the literature (about 20%). Failures in the sealing of vials, leaving them vulnerable to moisture and oxygen, or lack of an efficient stirring system might be possible explanations for these results. A suitable chemical reactor could solve the problem. Regarding polymer characterization, all techniques used not only confirmed the expected structure of the polymer, but also showed the highest proportion of lactide units compared to to glycolide units. (author)

  4. Development of methodology for the synthesis of poly(lactic acid-co-glycolic acid) for use in the production of radioactive sources; Desenvolvimento da metodologia para sintese do poli(acido latico-co-acido glicolico) para utilizacao na producao de fontes radioativas

    Energy Technology Data Exchange (ETDEWEB)

    Peleias Junior, Fernando dos Santos; Zeituni, Carlos Alberto; Rostelato, Maria Elisa Chuery Martins; Souza, Carla Daruich de; Mattos, Fabio Rodrigues de; Moura, Eduardo Santana de; Moura, Joao Augusto; Benega, Marcos Antonio Gimenes; Feher, Anselmo; Costa, Osvaldo Luiz da; Rodrigues, Bruna Teiga, E-mail: fernandopeleias@gmail.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP/CTR), Sao Paulo, SP (Brazil). Centro de Tecnologia das Radiacoes; Fechine, Guilhermino Jose [Universidade Presbiteriana Mackenzie, Sao Paulo, SP (Brazil). Escola de Engenharia

    2015-05-15

    According to the World Health Organization, cancer is a leading cause of death worldwide. A radiotherapy method extensively used in prostate cancer is brachytherapy, where the area requiring treatment receives radioactive seeds. Iodine-125 seeds can be inserted loose or stranded in bioabsorbable polymers produced from poly(lactic-co-glycolic acid) (PLGA). We developed the synthesis methodology for PLGA and the results obtained show that it was possible to determine the optimal reaction parameters (time and temperature) for PLGA in 80/20 (lactide/glycolide) ratio. The yield was higher than 90% using a temperature of 110 °C and reaction time of 72 hours; however, the molecular weight values obtained are very low compared to those obtained by other authors. New tests using previously synthesized dimers and nitrogen atmosphere are being performed. These conditions could potentially increase the molar mass of PLGA. All techniques used confirmed the expected structure of the polymer. (author)

  5. Obtención de concentrados de ácidos grasos poliinsaturados por el método de los compuestos de inclusión de urea

    Directory of Open Access Journals (Sweden)

    Robles Medina, A.

    1995-06-01

    Full Text Available A concentration process for polyunsaturated fatty acids (PUFAs n-3 stearidonic (18:4 n-3, eicosapentaenoic (20:5 n-3 and docosahexaenoic (22:6 n-3 from cod liver oil using urea method has been thoroughly studied. The influence of urea/fatty acid ratio and crystallization temperature have been studied on both fatty acids recovery yield and fatty acid concentration. Methanol and ethanol have been used as urea solvent. The use of methanol and an urea/fatty acid ratio of 4:1, and 4°C were found to be the best conditions for SA (concentration 8,5%, yield 71,6 and DHA (59,8% and 100%, respectively. However, 28ºC was found to be the best temperature for EPA (28,7% and 75,6%, respectively. At temperatures below -12ºC, PUFAs also developed adducts compounds, mainly adducts derived from EPA. A variance analysis showed the urea/fatty acid ratio as the main factor affecting fatty acid concentration. At the same time, high urea/fatty acid ratios resulted in increased influence of the solvent used, being more favorable for methanol.

    En este trabajo se estudia la influencia de la relación urea/ácidos grasos y de la temperatura de cristalización (con metanol y etanol como disolventes de la urea sobre las concentraciones y rendimientos en los ácidos grasos estearidónico (SA, 18:4n-3, eicosapentaenoico (EPA, 20:5n-3 y docosahexaenoico (DHA, 22:6n-3, utilizando ácidos grasos de aceite de hígado de bacalao. Las máximas concentraciones y rendimientos en SA (8,5% y 71,6%, respectivamente y en DHA (59,8% y 100% se han obtenido con metanol, una relación urea/ácidos grasos 4:1 p/p y 4°C; sin embargo, si se desean obtener altas concentraciones y rendimientos en EPA es preferible cristalizar a 28ºC (rendimiento 76%, concentración 29%. A temperaturas por debajo de -12°C también los PUFAs forman compuestos de inclusión en un porcentaje elevado, sobre todo el EPA. Mediante un análisis de la varianza se ha puesto de manifiesto que la variable con

  6. Inflamación celular en pacientes sometidos a cirugía electiva tratados con carbohidratos de baja carga glicémica, ácidos grasos omega 3 y antioxidantes

    Directory of Open Access Journals (Sweden)

    Lucrecia Arceo-Giorgana

    2016-03-01

    Full Text Available Antecedentes y Objetivos. La creciente complejidad de la Especialidad de Cirugía Plástica, ha inducido a su ramificación en varias subespecialidades. Una de las limitantes de la Cirugía Plástica ha sido la obesidad, enfermedad que conlleva complicaciones, aumenta el riesgo de inconvenientes y hace insuficiente el resultado proyectado para la estética y la función. El objetivo del presente trabajo es mostrar la importancia de la evaluación de la inflamación celular en la preparación de pacientes sometidos a cirugía electiva, previamente tratados con dieta con carbohidratos de baja carga glicémica, ácidos grasos omega 3 y 6 y antioxidantes. Material y Método. Realizamos un estudio longitudinal prospectivo cuasi-experimental no aleatorio de 23 pacientes que solicitaron intervenciones de Cirugía Plástica y aceptaron entrar en el protocolo de diagnóstico y tratamiento de inflamación celular. Primero realizamos el cuestionario de Reporte de Inflamación Celular "RIS" y tomamos a los pacientes pruebas de inflamación celular y de ácido eicosapentaenóico (EPA, ácido decosaexaenóico (DHA, acido araquidónico (AA y ácido dihomogamalinoléico (DGLA al ingreso y antes de la cirugía. Analizamos los datos con estadística descriptiva y comparamos los rangos con la prueba McNemar y de hipótesis t de student, del sistema SPSS. Resultados. Tras aplicar el RIS a los 23 pacientes antes de la preparación con dieta y tras un periodo de 1 a 3 meses, evaluamos las respuestas de cada uno de los cuestionarios a través de las pruebas no paramétricas, encontrando diferencia significativa en los 14 items. Se mejoró considerablemente a la alza el EPA; el DHA y el AA sin cambios significativos; y el DGLA disminuyó considerablemente. Con t de student encontramos variación significativa en los fosfolípidos del plasma y no hubo diferencia significativa entre los DHA y AA. Conclusiones. Demostramos así la efectividad de la dieta de los omegas

  7. Fuentes alimentarias y adecuación de la ingesta de ácidos grasos omega-3 y omega-6 en una muestra representativa de adultos españoles

    Directory of Open Access Journals (Sweden)

    Rosa M. Ortega Anta

    2013-12-01

    Full Text Available Introducción y Objetivos: Teniendo en cuenta la importancia sanitaria del aporte de ácidos grasos omega-3 y omega-6, y ante la escasez de estudios sobre el tema en colectivos españoles, se plantea la conveniencia de conocer la ingesta de estos ácidos grasos, su adecuación a los objetivos nutricionales marcados y sus fuentes alimentarias en una muestra representativa de la población española. Métodos: Se ha estudiado un colectivo de 1068 adultos (521 varones y 547 mujeres de 17 a 60 años, seleccionados en diez provincias españolas, que constituyen una muestra representativa de la población, a nivel nacional. Se determino la ingesta de ácidos grasos poliinsaturados (AGP, ácidos grasos omega-3, α-linolénico (ALA, ácido eicosapentaenoico (EPA y docosahexaenoico (DHA, ácidos grasos omega-6, ácido linoléico (LA y araquidónico, en g/día y en porcentaje de la energía, utilizando un "Registro del consumo de alimentos" durante 3 días consecutivos, incluyendo un domingo, y recogiendo también datos personales, sanitarios y antropométricos de los individuos estudiados. Resultados y Discusión: La ingesta de grasa total y grasa saturada fue superior a la marcada como aconsejable en el 89,2% y 93,3% de los individuos, respectivamente, mientras que con la ingesta de AGP sucede lo contrario, siendo más frecuente el aporte insuficiente (79,2% de los estudiados tienen una ingesta inferior al 6% de la energía. Resulta especialmente bajo el aporte de ácidos grasos omega-3 (1,85 ± 0,82 g/día, que proporcionan menos del 1% de la energía en el 85,3% de los individuos. En concreto el ALA (1,40 ± 0,55 g/día no supera el 0,5% de la energía en el 53,7% de los casos y la suma de EPA+DHA (0,55 ± 0,58 g/día no supera los 0,5 g/día en el 64,6%. Por otra parte, el aporte de ácidos grasos omega-6 fue más adecuado (10,95 ± 3,79 g/día y en concreto el de LA (10,77 ± 3,76 g/día supuso menos del 3% de la energía en el 25,5% de los

  8. CHARACTERIZATION OF THE NUTRITIONAL QUALITY OF THE MEAT IN SOME SPECIES OF CATFISH: A REVIEW REVISIÓN: CARACTERIZACIÓN DE LA CALIDAD NUTRICIONAL DE LA CARNE EN ALGUNAS ESPECIES DE BAGRE

    Directory of Open Access Journals (Sweden)

    Nubia Estella Cruz Casallas

    2012-06-01

    se encuentra dentro de lo establecido por la Organización Mundial de la Salud (OMS para muchas de estas especies de bagre. De igual forma, el contenido de los ácidos eicosapentaenoico (EPA y docosahexaenoico (DHA, así como el de aminoácidos, minerales y vitaminas, revelan una alta variabilidad individual y entre especies, asociada con el tipo de cultivo y los hábitos alimenticios, así como también con la edad y peso al momento del sacrificio. Asimismo, se han definido algunos parámetros de calidad relacionados con la susceptibilidad a la autolisis, oxidación e hidrólisis de las grasas y con las alteraciones causadas por microorganismos que generan cambios determinantes en las características fisicoquímicas, microbiológicas y sensoriales. Esta revisión recopila la información actual relacionada sobre la composición nutricional de la carne de bagre y los parámetros de calidad.

  9. Influence of omega-3 fatty acids from the flaxseed (Linum usitatissimum on the brain development of newborn rats Influencia de los ácidos grasos omega-3 de la linaza (Linum usitatissimum en el desarrollo del cerebro de ratas recién nacidas

    Directory of Open Access Journals (Sweden)

    K. C. Lenzi Almeida

    2011-10-01

    base de caseína, y GCM: con caseína modificada suplementada con fibras y aceite de soja. Las crías recién nacidas fueron pesadas y sometidas a eutanasia; los cerebros fueron recolectados para la evaluación del peso y el perfil lipídico mediante cromatografía gaseosa. Resultados: Se verificó aumento significativo en el peso cerebral (39% y en el peso relativo del cerebro (37% en las crías de madres alimentadas con la dieta de linaza. Los ácidos grasos omega-3 (n-3 de la linaza se encontraron en abundancia en la dieta elaborada con esta oleaginosa, y también hubo importante aumento en el ácido docosahexaenoico (DHA (38%, así como en el total de ácidos grasos omega-3 (n-3 (62%. Conclusión: La dieta materna a base de linaza durante el embarazo influye en la incorporación de ácidos grasos omega-3 en la composición del tejido cerebral, asegurando un buen desarrollo de este órgano en ratas recién nacidas.

  10. NCBI nr-aa BLAST: CBRC-PHAM-01-1048 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PHAM-01-1048 ref|YP_003183601.1| hypothetical protein Aaci_0148 [Alicyclobacillus acido...caldarius subsp. acidocaldarius DSM 446] gb|ACV57212.1| hypothetical protein Aaci_0148 [Alicyclobacillus acido...caldarius subsp. acidocaldarius DSM 446] YP_003183601.1 6e-05 22% ...

  11. Composición molecular de acidos humicos evaluada mediante pirólisis -cromatografia de gases- masas e hidrólisis térmica asistida y metilación, en suelos altoandinos – colombia

    OpenAIRE

    Claudia Martinez; Isabel Bravo; Francisco Martin

    2014-01-01

    Se determinó la composición  molecular de ácidos húmicos (AH), extraídos del horizonte A de suelos altoandinos, Colombia dedicados a tres usos: bosque primario, cultivo y pastura. Los AH se obtuvieron mediante extracción secuencial de la materia orgánica humificada (MOH) con soluciones de tetraborato, pirofosfato e hidróxido de sodio (0,1N), y fueron purificados por diferentes procesos analíticos. Su composición molecular se determinó  por Py-GC/MS y THMA. Los diferentes productos de la pirol...

  12. Shyntesis and cytotoxicity evaluation in vitro of new compounds with hybrid structures of 8-flavoneacetic acid and quinolones; Sintesis y evaluacion citotoxica in vitro de nuevos compuestos con estructuras hibridas del acido 8-flavonacetico quinolonas

    Energy Technology Data Exchange (ETDEWEB)

    Biaa, M.F.; Castellano, J.M.; Emling, F.; Schlick, E. [Knoll, S.a., Madrid (Spain)

    1994-12-31

    Using the structural similarity between 8-flavoneacetic acid the antitumor quinolones, we have prepared some hybrid compounds on both systems and studied their cytotoxicity. None of the sinthesized compounds have shown sufficient interest for further development. 33 refs.

  13. Radiation characterization, physico-chemical and mineralogical materials associated with the production of phosphoric acid; Caracterizacion radiactiva, fisico-quimica y mineralogica de materiales asociados con la proudccion del acido fosforico

    Energy Technology Data Exchange (ETDEWEB)

    Pereira Ambrosio, E.; Gazquez Gonzalez, M.; Bolivar Raya, J. P.

    2011-07-01

    For over 20 years there is a clear awareness in the scientific community about the need to assess the occupational and environmental radiological impact caused by the activities of conventional nuclear industries (industries NORM). These are characterized either by use in their production processes raw materials rich in natural radionuclides, or by generating commercial products, by-products or residues enriched in these radionuclides. (Author)

  14. Obtention of Samarium and Gadolinium concentrates by solvent extraction using mono-2-ethylhexyl ester of 2-ethylhexyl phosphonic acid; Obtencao de concentrados de samario e gadolinio via extracao por solventes com o ester mono-2-etilhexil do acido 2-etilhexilfosfonico

    Energy Technology Data Exchange (ETDEWEB)

    Miranda Junior, Pedro

    1996-07-01

    The rare earth chlorides solution employed in this study, which is constituted by medium and heavy fractions, is derived from monazite processing accomplished by NUCLEMON-Mineroquimica (SP). This solution shows an acidity about 1.18 M and 189 g/L of rare earth oxides, containing as main constituents: Sm(34.55%), Gd(23.85%), Dy (6.82%), and Y (24.45%). It was used, as organic phase, 2-ethylhexyl phosphonic acid, mono-2-ethylhexylester diluted to 1 M in isododecane. (author)

  15. Cleaning of the diffusers with formic acid for aeration of an active sludge process; Limpieza con acido formico de los difusores demembrana para la aireacion en un proceso de fangos activos

    Energy Technology Data Exchange (ETDEWEB)

    Simon Andre, P.; Lardin Mifsut, C.; Abella Solar, M.; Marc Ponsoda, J.; Garcia del Real, A.; Perez Sanchez, P.

    2009-07-01

    Electric energy costs are one of the main issues inside the total amounts in a WWTP, being the aeration system consumption the highest one but also the most flexible and order to reduce the global costs. In this work it has been quantified the improvement obtained when formic acid is used to do periodic cleanness of the diffusers. When this is used, manual cleanness can be kept apart. During the cleanness processes with formic acid, it has been observed a reduction in the pressure drop, between 6 and 13%, and a reduction in the power demand, between 7 and 12% . These reduction have been correlated with the annual costs, reaching an annual saving of near 3%. (Author) 8 refs.

  16. Polymeric blends from post-consumer PET and polyester becoming of glycerol and phthalic acid; Misturas polimericas a partir do PET pos-consumo e poliesteres derivados do gliceraol e acido ftalico

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, C.S.; Brioude, M.; Fiuza, R.P.; Luporini, S.; Carvalho, R.F.; Jose, N.M., E-mail: cleidienesm@gmail.co [Universidade Federal da Bahia (GECIM/UFBA), Salvador, BA (Brazil). Inst. de Quimica. Grupo de Energia e Ciencias dos Materiais

    2010-07-01

    Preparation of physical mixture or polymer blends is a very important method to obtain a final product with excellent balance of properties, where one component can compensate for the poor property of another, and is often a modified low cost compared to development and synthesis of a new polymer. PET has become a major waste of post-consumer plastics and aiming to remedy this problem, this work aims to obtain blends from recycled PET and polyesters derived from glycerol and phthalic acid. The material with higher proportion of PET showed better thermal properties, observed by TGA and DSC, with a similar profile of pure PET. In XRD analysis showed a semicrystalline, while the SEM is a smooth surface on all materials, characteristic of pure polyester. The ratio of 50% its surface showed a probable immiscibility of polymers. (author)

  17. Influence of acids composition in the properties of poly(glycerol citrate-co-adipate); Influencia da composicao dos acidos nas propriedades do poli(glicerol citrato-co-adipato)

    Energy Technology Data Exchange (ETDEWEB)

    Brioude, M.M.; Pereira, R.; Fiuza, R.P.; Jose, N.M., E-mail: mbrioude@gmail.co [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Quimica

    2010-07-01

    Synthetic polymers are highly resistant to degradation, being one of the greatest responsible for environment pollution. Consequently, an increase in biodegradable polymers research and development is happening. In this work we propose the synthesis and characterization of polyesters potentially biodegradable using glycerol, citric acid (CA) and adipic acid (AD). The polyesters were prepared by adding the reactants in molar ratio between glycerol:acids (1:1,5), but changing the acids ratio. The system was heated to 150-160 deg C, under nitrogen atmosphere and magnetic stirring, without catalyst adding. The samples were characterized by TGA, DSC, FTIR, XRD e SEM. The results show that the polymers prepared are amorphous polyesters, with two thermal events in 250 deg C and 450 deg C and the surfaces presents smooth and rough regions refers to bonds between glycerol and CA or AD, respectively. (author)

  18. COMPOSICIÓN MOLECULAR DE ACIDOS HUMICOS EVALUADA MEDIANTE PIRÓLISIS -CROMATOGRAFIA DE GASES- MASAS E HIDRÓLISIS TÉRMICA ASISTIDA Y METILACIÓN, EN SUELOS ALTOANDINOS – COLOMBIA

    Directory of Open Access Journals (Sweden)

    Claudia Martinez

    2014-07-01

    Full Text Available Se determinó la composición  molecular de ácidos húmicos (AH, extraídos del horizonte A de suelos altoandinos, Colombia dedicados a tres usos: bosque primario, cultivo y pastura. Los AH se obtuvieron mediante extracción secuencial de la materia orgánica humificada (MOH con soluciones de tetraborato, pirofosfato e hidróxido de sodio (0,1N, y fueron purificados por diferentes procesos analíticos. Su composición molecular se determinó  por Py-GC/MS y THMA. Los diferentes productos de la pirolisis se clasificaron atendiendo a la familia química a la que pertenecen,  prevalecieron los ácidos grasos (FA como el producto más abundante en los AH de todos los usos, mientras que  los fenoles, esteroles, S-compuestos y terpenos se encontraron en menor abundancia. Se presentaron diferencias en los porcentajes de abundancia relativa de los grupos identificados, demostrando que el cambio de uso de suelo influye en las propiedades físicas, químicas y biológicas del suelo por lo que se perciben diferentes grados de transformación, que afectan la composición de los AH y su reactividad en el suelo.

  19. Obtention and characterization of acrylic acid-i-polyethylene organometallic copolymers with Mo, Fe, Co, Zn, and Ni; Obtencion y caracterizacion de copolimeros organometalicos de acido acrilico-i-polietileno, con Mo, Fe, Co, Zn y Ni

    Energy Technology Data Exchange (ETDEWEB)

    Dorantes, G.; Urena, F.; Lopez, R. [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico); Lopez, R. [Universidad Autonoma del Estado de Mexico (Mexico)

    1997-07-01

    In this study a graft acrylic acid (AA) in low density polyethylene (PEBD) copolymers were prepared, using as reaction initiator, gamma radiation at different doses. These copolymers were coordinated with molybdenum, cobalt, iron, zinc and nickel. the obtained polymeric materials were characterized by conventional analysis techniques. It was studied the measurement parameter variation of the positron annihilation when they inter activated with this type of materials and so obtaining information about microstructure of these polymers. (Author)

  20. Chemical characterization of composites developed from glycerol and dicarboxylic acids rein forced with piassava fiber; Caracterizacao quimica de compositos desenvolvidos a partir do glicerol e acidos dicarboxilicos reforcados com fibra de piacava

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, Cleidiene S.; Oliveira, Jamerson C.; Guimaraes, Danilo H.; Jose, Nadia M., E-mail: cleidienesm@gmail.com [Universidade Federal da Bahia, Instituto de Quimica, GECIM - Programa de Pos-Graduacao em Engenharia Quimica, Salvador, BA (Brazil); Carvalho, Ricardo F. [Universidade Federal da Bahia, Escola Politecnica, Mestrado em Engenharia Ambiental Urbana, Salvador, BA (Brazil)

    2011-07-01

    In search of alternative technologies that enable the use of products with lower environmental impact, This study aims to develop a composite polymer-based piassava fiber. The sludge, waste and byproduct of commercial uses currently being used as reinforcement in polymer matrices, due to presence of lignocellulosic materials. The matrix polymer used was synthesized from glycerol with dicarboxylic acids, in order to open future perspectives on the use of glycerin generated from purified biodiesel production plastics. Composites with 2, 5, 10 wt% of piassava fiber cut into 5 mm raw and treated were obtained a mixture of solution. The materials were characterized by TGA, DSC, XRD and SEM. It was observed that the material under study is promising for the industrial market, because it has good compatibility with natural fibers allowing wider application of fiber natural and glycerol, producing semicrystalline composites and with good thermal properties. (author)

  1. Modified montmorillonite as a heterogeneous catalyst in (m)ethyl esterification reaction of lauric acid; Montmorilonita modificada como catalisador heterogeneo em reacoes de esterificacao (M)etilica de acido laurico

    Energy Technology Data Exchange (ETDEWEB)

    Zatta, Leandro; Nepel, Angelita; Barison, Andersson; Wypych, Fernando, E-mail: wypych@ufpr.br [Departamento de Quimica, Universidade Federal do Parana (UFPR), Curitiba , PR (Brazil)

    2012-07-01

    Montmorillonite was modified with zirconium polyoxycations in the presence of ammonium sulphate. The material was characterized and used as a catalyst in the esterification of lauric acid, the reactions being accompanied by 2{sup 3} factorial design. Conversions of up to 95.33 and 83.35% were observed for the methyl and ethyl esterification reactions respectively, proving superior to results obtained by thermal conversion. The material was submitted to three reaction cycles and similar conversions were observed, indicating the catalyst is not significantly deactivated after reuse. The catalyst was also tested under reflux conditions, yielding a maximum conversion of 36.86%. (author)

  2. Esterification of fatty acids using sulfated zirconia and composites activated carbon/sulfated zirconia catalysts; Esterificacao de acidos graxos utilizando zirconia sulfatada e compositos carvao ativado/zirconia sulfatada como catalisadores

    Energy Technology Data Exchange (ETDEWEB)

    Brum, Sarah S.; Santos, Valeria C. dos; Destro, Priscila; Guerreiro, Mario Cesar [Universidade Federal de Lavras, MG (Brazil). Dept. de Quimica

    2011-07-01

    In this work sulfated zirconia (SZr) and activated carbon/SZr composites produced by impregnation method with or without heating treatment step (CABC/SZr-I and CABC/SZr-I SC) and by the method of synthesis of SZr on the carbon (CABC/SZr-S) was used as catalysts in the esterification reactions of fatty acids. The SZr presented very active, conversions higher than 90% were obtained after 2 h of reaction. The activity of the composite CABC/SZr-I20%SC was up to 92%, however, this was directly related to time and temperature reactions. CABC/SZr-I and CABC/SZr-S were less active in esterification reactions, what could be attributed to its low acidity. (author)

  3. The elution of erbium from a cation exchanger bed by means of the N-hydroxyethyl-ethylene-diamine triacetic acid; Mecanismo de la elucion del erbio en un cambiador cationico con el acido n-hidroxietil-etilen-diamono-triacetico

    Energy Technology Data Exchange (ETDEWEB)

    Amer Amezaga, S.

    1963-07-01

    A physicochemical study of the phenomena resulting when erbium is eluted from a cation-exchanger bed at a steady by means of the N-hydroxyethyl-ethylene-diamine-triacetic acid (HEDTA) is made. Two different retaining beds are used, a hydrogen bed, in which no ammonium passes through, and a zinc bed, which leaks ammonium ion. Good agreement between experimental and calculated values by using the equations deduced for the concentrations of the main species has been achieved, with errors around 1-2% in most of the experiments. (Author) 69 refs.

  4. Crystal structure of ATVORF273, a new fold for a thermo- and acido-stable protein from the Acidianus two-tailed virus

    DEFF Research Database (Denmark)

    Felisberto-Rodrigues, Catarina; Blangy, Stéphanie; Goulet, Adeline;

    2012-01-01

    in the viral world. To understand this intriguing phenomenon, we have undertaken structural studies of ATV virion proteins and here we present the crystal structure of one of these proteins, ATV[Formula: see text]. ATV[Formula: see text] forms tetramers in solution and a molecular envelope is provided...... for the tetramer, computed from small-angle X-ray scattering (SAXS) data. The crystal structure has properties typical of hyperthermostable proteins, including a relatively high number of salt bridges. However, the protein also exhibits flexible loops and surface pockets. Remarkably, ATV[Formula: see text......] displays a new [Formula: see text] protein fold, consistent with the absence of homologues of this protein in public sequence databases....

  5. Oscillatory dynamics in systems containing bromate and 1,4-cyclohexanedione in acidic media. I. The effect of temperature; Dinamica oscilatoria em sistemas contendo bromato e 1,4-ciclo-hexanodiona em meio acido. I. Efeito da temperatura

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Hyrla C.L.; Oliveira, Tatiane B.; Varela, Hamilton, E-mail: varela@iqsc.usp.br [Instituto de Quimica de Sao Carlos, Universidade de Sao Paulo (IQSC/USP), Sao Carlos, SP (Brazil)

    2012-07-01

    We present in this work the influence of temperature on the dynamics of homogeneous chemical systems containing bromate and 1,4-cyclohexanedione (1,4-CHD) in acidic media. In particular, the following systems were studied: bromate/1,4-CHD/acid, bromate/1,4-CHD/ferroin/acid and bromate/1,4-CHD/trisbipyridine ruthenium/acid. Investigations were carried out by means of an electrochemical probe, at five temperatures between 5 and 45 degree C. Activation energies (Ea) were estimated in different ways for the pre-oscillatory and oscillatory regimes. In any case, the Ea was found to depend on the catalyst, composition and initial concentrations. In addition, it was observed that ferroin and trisbipyridine ruthenium act as catalysts only during the transition between the induction period and oscillatory regime. (author)

  6. Acido tranexâmico e hemostasia em cirurgia de revascularização do miocárdio com circulação extracorpórea Tranexamic acid and hemostasis in myocardial revascularization with extracorporeal circulation

    Directory of Open Access Journals (Sweden)

    Guilherme F Vargas

    1992-12-01

    Full Text Available O antífibrinolítico sintético ácido tranexâmico (Transamin ® foi avaliado em seus efeitos hemostáticos e poupadores de transfusões homólogas, em pacientes submetidos a revascularização do miocárdio com circulação extracorpórea (CEC. Quarenta pacientes receberam placebo e 55 pacientes foram operados sob o efeito do ácido tranexâmico na dose de 10 g endovenosa no trans-operatório (2 g administrados na indução anestésica e os restantes 8 g nas 4 horas seguintes de cirurgia, de modo contínuo. O ácido tranexâmico, na dosagem utilizada, demonstrou possuir efeito hemostático impressionante, promovendo uma redução no débito pelos drenos torácicos da ordem de 47% nas 12 horas de P.O., 42,5% nas 24 horas de P. O. e 40,5% até a retirada dos drenos, em relação ao grupo-controle (pThe synthetic antif ibrinolytic drug tranexamic acid was evaluated in its hemostatic and blood saving effects, in patients submitted to myocardial revascularization with extracorporeal circulation. To 40 patients were administered placebo and to 55 tranexamic acid I.V. in a dosage of 10 g in the operative period (2 g in the anesthetic induction and the remaining 8 g in a continuous way during the operative procedure. Tranexamic acid, in this dosage, has proved to have a very impressive hemostatic effect, leadir g to a reduction in post operative bleeding of 47% in 12 h, 42,5% in 24 h and 40,5% when drains were taken off, related to the control group (p < 0.05. Tranexamic acid have led to less utilization for homologous paked red cells per patient, but statistical significance was found only in the 24 h of post operative period, with 1,025 units/patient in control group and 0,333 units/ patient in treated group. Concerning post operative complications, there have been more neurological alterations without sequelae (2.5% against 12.7% and creatinin alterations (5% against 10.9% in the tranexamic acid group. Such alterations were attributed to the high dosage used. As a conclusion, we do not recommend routine use of tranexamic acid to patients submitted to myocardial revascularization in the dosage of 10 g I.V., but, owing to the evident hemostatic effect of the drug, we recommend more investigations concerning the ideal dosage and way of administration.

  7. Phosphorus in chronosequence of burnt sugar cane in Brazilian cerrado: humic acid analysis by {sup 31}P NMR; Fosforo em cronossequencia de cana-de-acucar queimada no cerrado goiano: analise de acidos humicos por RMN de {sup 31}P

    Energy Technology Data Exchange (ETDEWEB)

    Rossi, Celeste Q.; Pereira, Marcos G.; Garcia, Andreas C., E-mail: mgervasiopereira@gmail.com [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Dept. de Solos; Perin, Adriano; Gazolla, Paulo R. [Instituto Federal de Educacao, Ciencia e Tecnologia Goiano, Rio Verde, GO (Brazil); Gonzalez, Antonio P. [Universidade de Coruna, ES (Spain). Faculdad de Ciencias

    2013-10-01

    The aim of this study was to identify, with the use of {sup 31}P NMR spectroscopy, organic P species in humic acids (HA) in samples from Oxisol cultivated in chronosequence with sugar cane, pasture and Cerrado. The main forms of P-type found were orthophosphate, monoester-P (phosphate sugars) and P-diester (orthophosphate). The {sup 31}P NMR technique proved capable of identifying changes in the areas studied as a function of sugar cane burning time. In areas with 1 and 5 years of burnt cane, a decrease in recalcitrant organic P in humic acids indicated the need for use of P-humic substances for plant nutrition (author)

  8. Gamma radiation effects at color, antioxidant capacity and fatty acid profile in peanut (Arachis hypogaea L.); Efeitos da radiacao gama na cor, capacidade antioxidante e perfil de acidos graxos em amendoim (Arachis hypogaea L.)

    Energy Technology Data Exchange (ETDEWEB)

    Camargo, Adriano Costa de; Canniatti-Brazaca, Solange Guidolin; Mansi, Debora Niero; Domingues, Maria Antonia Calori [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil). Dept. de Agroindustria, Alimentos e Nutricao; Arthur, Valter, E-mail: arthur@cena.usp.b [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

    2011-01-15

    Irradiation is efficient at extinction fungi contamination in peanuts. Peanuts have high biologic value protein, minerals, vitamin E, complex B, and high concentration of lipids. The objective of this research is to evaluate the gamma irradiation effect on color, total phenolic, antioxidant activity, and fatty acid profile in peanuts (Arachis hypogaea L.). Cultivars IAC-Tatu ST and IAC-Runner 886 were submitted to gamma radiation with doses of 5.0; 7.5; 10.0, and 15.0 kGy and storage at room temperature. There was no significant difference in the color of IAC-Tatu ST. However, significant difference was found in the luminosity and Chroma in IAC-Runner 886. Total fenolics differed from the control with 33.27 mg.g{sup -1} and treatment dose of 10.0 kGy with 58.60 mg.g{sup -1} in IAC-Tatu ST. This parameter not had significant difference in IAC-Runner 886 and the control with 51.59 mg.g{sup -1}. The antioxidant activity did not present significant difference with a dose of 10.0 kGy, recommended for the elimination of fungi in peanuts. The dose of 10.0 kGy showed a decrease in saturated fatty acids, increase in unsaturated fatty acids, and an increase in linolleic acid. The oleic/linoleic relation decreased justifying further research correlating storage and oxidative stability. (author)

  9. Bioethanol production by cashew apple bagasse (Anacardium occidentale L.): comparison of acid diluted and alkali pre-treatments; Producao de bioetanol a partir da fibra do caju (Anacardium occidentale L.): comparacao entre o pre-tratamento acido e alcalino

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigues, Tigressa H.S.; Pinheiro, Alvaro D.T.; Goncalves, Luciana R.B. [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil); Rocha, Maria V.P.; Macedo, Gorete R. de [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil)

    2008-07-01

    Due to the growing environmental awareness on the negative impact resulting from utilization of fossil fuels, and in the search for renewable energy sources, biofuels' interest as Bioethanol has rapidly expanded recently. In this context, lignocellulosic compounds have become center of attention as an abundant and economic alternative source of carbohydrates for ethanol production. In this study, cashew's bagasse acid hydrolysis was initially studied for glucose synthesis and its fermentation towards ethanol production. Sulfuric acid concentration, solids concentration and time were some of the factors evaluated. The highest glucose productivity value (162,9 mg.g de bagaco{sup -1}) , was obtained for 0,6 mol.L{sup -1} of sulfuric acid in an autoclave at 121 deg C for 15 min. For the fermentation of the hydrolyzed material by S. cerevisiae containing 16 {+-} 2,0 g.L{sup -1} of glucose metabolic, the yield and productivity obtained were 0,63 g-g glucose{sup -1} and 1,43 g.L{sup -1}h{sup -1} respectively. Ethanol concentration after 6 h of fermentation of this hydrolyzed was 11 g.L{sup -1}. In the best conditions of acid hydrolysis, a second pre-treatment with diluted sulfuric acid was performed to evaluate availability of hemicelluloses at 160 deg C and 180 deg C. For comparative purposes, alkali pre-treatment was evaluated under the conditions of 0,6 mol.L{sup -1}, 30% p/v of bagasse thermically treated at 121 deg C for 15 minutes. However, concentration of sugars was not sufficient for fermentation. Also, nitrogen supplementation of the hydrolyzed fraction did not influence significantly ethanol production. The results demonstrate that the hydrolyzed fraction from the acid pre-treatment of cashew's bagasse could be utilized for ethanol production. (author)

  10. Evaluation of gamma irradiation effects on carotenoids, ascorbic acid and sugar contents of buriti fruit (Mauritia flexuosa L.); Avaliacao dos efeitos da radiacao gama nos teores de carotenoides, acido ascorbico e acucares do fruto Buriti do Brejo (Mauritia flexuosa L.)

    Energy Technology Data Exchange (ETDEWEB)

    Lima, Antonio Luis dos Santos [Instituto Militar de Engenharia (IME), Rio de Janeiro, RJ (Brazil). Secao de Engenharia Quimica, Quimica Organica e de Produtos Naturais], e-mail: santoslima@ime.eb.br; Lima, Keila dos Santos Cople; Silva, Jaqueline Michele [Instituto Militar de Engenharia (IME), Rio de Janeiro, RJ (Brazil). Secao de Engenharia Nuclear. Irradiacao de Alimentos], e-mail: keila@ime.eb.br, e-mail: jaquelinefisica@bol.com.br; Coelho, Maysa Joppert [Instituto Militar de Engenharia (IME), Rio de Janeiro, RJ (Brazil). Secao de Engenharia Nuclear. Engenharia Ambiental], e-mail: maysa@ime.eb.br; Godoy, Ronoel Luiz de Oliveira [EMBRAPA Agroindustria de Alimentos, Rio de Janeiro, RJ (Brazil). Lab. de Cromatografia Liquida, Quimica Organica e de Produtos Naturais], e-mail: ronoel@ctaa.embrapa.br; Pacheco, Sidney [EMBRAPA Agroindustria de Alimentos, Rio de Janeiro, RJ (Brazil). Lab. de Cromatografia Liquida, Ciencia e Tecnologia de Alimentos], e-mail: sidney@ctaa.embrapa.br

    2009-07-01

    Buriti (Mauritia flexuosa L.), a typical fruit from the Northeast and Center-West Amazon of Brazil, is used in many regional dishes. It is considered an excellent source of carotenoids that are A vitamin precursors, showing a majority of beta-carotene. It also presents ascorbic acid and sugar contents. Many studies have indicated that the lack of A vitamin is the main cause of night blindness and xerophthalmia. Also, ascorbic acid deficiency may cause scorbutic disease. The use of food irradiation is growing and represents an economic benefit to agriculture through the reduction of post-harvesting losses while maintaining food nutritional quality. In this study, buriti in natura was treated with gamma irradiation with doses of 0.5 kGy and 1.0 kGy. The objective was to evaluate the irradiation effects on total carotenoids, ascorbic acid and sugars concentrations of buriti. The fruit was evaluated through the total carotenoids analysis, by spectrophotometry, and the carotenoids (alpha and beta-carotene and lutein), ascorbic acid and sugars were determined by high performance liquid chromatography (HPLC). The results showed that buriti is an excellent source of total carotenoids (44600 {mu}g/100 g). The irradiation of buriti with the dose of 0.5 kGy did not significantly change carotenoids and sugars contents. However, there was a reduction of ascorbic acid concentration with an increase of the dose, which may have been caused by irradiation or by intrinsic and extrinsic factors that alter ascorbic acid stability in food, converting ascorbic to dehydroascorbic acid, while keeping the C vitamin active form. (author)

  11. Dosimetric influence of hyaluronic acid in patients with prostate cancer treated with RT3D; Influencia dosimetrica del acido hialuronico en pacientes cancer de prostata tratados con RT3D externa+HDR braquiterapia

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, J.; Vazquez, J. A.; Garcia Blanco, A. S.; Gomez, F.; Montejo, A.; Raba, J. I.; Pacheco, M. T.; Cardenal Carro, J.; Mendigueren, M. A.

    2013-07-01

    This study aims to assess the influence on dosimetry by the introduction of hyaluronic acid and assess the need for the realization of a second CT scan and consequent re-planning of RT3D treatment. (Author)

  12. Exhaust lead-acid batteries recycling as a tool of the environmental protection policy. Energy, environmental and economic issues; Il riciclaggio delle batterie al piombo-acido esauste come strumento della politica di salvaguardia ambiental. Aspetti energetici, ambientali ed economici

    Energy Technology Data Exchange (ETDEWEB)

    Picini, P.; Battista, A. [ENEA, Divisione Caratterizzazione dell' Ambiente e del Territorio, Centro Ricerche Casaccia, S. Maria di Galeria, RM (Italy)

    2001-07-01

    Lead is an heavy metal that has a major impact on human health and his removal from the environment is an important action for its protection. The aim of the present work is to provide a framework of the Italian lead recycling with respect to the economic and environmental aspects of COBAT activities (COBAT is the Mandatory Consortium to collect and recycle the exhaust lead-acid batteries and lead wastes). In order to better understand the context in which COBAT works, some statistical data on the lead production, consumption and end uses in Italy and in the world are provided. An estimate of the energy consumptions and the environmental impact related to Italian lead production was also carried out. [Italian] Il piombo e' uno dei metalli pesanti a maggiore impatto ambientale e sanitario e la sua rimozione dall'ambiente costituisce un'importante azione di protezione e tutela della salute umana. Lo scopo del presente lavoro e' quello di fornire un quadro di riferimento relativo al riciclaggio del piombo in Italia evidenziandone gli aspetti ambientali ed economici in relazione alle attivita' condotte dal COBAT (Consorzio Obbligatorio delle Batterie Esauste e dei rifiuti piombosi). In tal senso, per disporre di una visione piu' completa del contesto in cui si inserisce l'attivita' del Consorzio, vengono forniti alcuni dati di carattere statistico sulla produzione, sul consumo e sugli utilizzi del piombo in Italia e nel mondo e viene effettuata una stima dei consumi energetici e dell'impianto ambientale associati alla produzione di piombo nazionale.

  13. Mechanical performance evaluation of bionanocomposites with polymeric matrix of poly(lactic acid)-PLA and organo clay; Avaliacao do comprtamento mecanico de bionanocompositos com patriz polimerica de poli(acido latico)-PLA e argila organofilica

    Energy Technology Data Exchange (ETDEWEB)

    Cavalcanti, Shirley N.; Melo, Tomas J.A. de; Araujo, Edcleide M.; Oliveira, Akidauana D.A.; Lima, Jessica C.C., E-mail: shirleynobre@gmail.com [Universidade Federal de Campina Grande - UFCG - Dept. de Engenharia de Materiais, Campina Grande, PB (Brazil)

    2011-07-01

    The aim of this study is to evaluate the influence of ethylene/methyl acrylate (EMA) and ethylene/glycidyl methacrylate (E-GMA) copolymers and organoclay on the mechanical properties of polymer PLA-poly (lactic acid), from the system PLA / organoclay. The clay was organophilized with the ionic surfactant praepagem and the mixtures were prepared in a modular twin screw extruder. The behavior of bionanocomposites was evaluated by tensile and impact tests. The results of FTIR and DR-X indicated that the process was efficient organophilization. The mechanical properties showed that the elastic modulus and tensile strength of bionanocomposites obtained decreased compared to PLA. On the other hand there was a gain for resistance to impact, for all systems except for system PLA/OMMT. (author)

  14. Viability of uses of fluoride 10% acid to removal paramagnetic ions in soil samples and its physic fractions; Viabilidade do emprego de acido fluoridrico 10% para a remocao de ions paramagneticos em amostras de solos e suas respectivas fracoes fisicas

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Larissa Macedo dos; Simoes, Marcelo Luiz; Martin-Neto, Ladislau, E-mail: larissa@cnpdia.embrapa.b [EMBRAPA Instrumentacao Agropecuaria, Sao Carlos, SP (Brazil); Montes, Celia Regina [Universidade de Sao Paulo (IQ/USP), SP (Brazil). Inst. de Quimica; Melfi, Adolpho Jose [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil)

    2010-09-15

    Treated sewage effluent utilization for irrigation represents an antique, popular and attractive alternative to the common disposal of effluent to watercourses and includes three main purposes: effluent complementary treatment, water and nutrient source to the soil-plant system. However, because in Brazil no experiences in wastewater recycling exists consequently few scientific studies were carried out despite the importance of the subject. The present study aimed to evaluate by chemical and spectroscopy techniques the soil organic matter from soils irrigated with treated sewage effluent. Five treatments were evaluated: TSI (control) - irrigation with potable water and addition of the nitrogen as mineral fertilizer; T100, T125, T150 and T200 - irrigation with treated sewage sludge effluent and addition of the nitrogen as mineral fertilizer. The results obtained shown changes in the carbon contend and humification degree for the soils irrigated with treated sewage sludge effluent due to the increase activity of the decomposition of organic matter, stimulated by the increase of water in the soil. (author)

  15. Modificacion del Valor Nutrimental de Acidos Grasos en Semilla de la Halofita Salicornia bigelovii Torr. con la Interaccion de Bacterias Promotoras del Crecimiento de Plantas (Azospirillum halopraeferens y Klebsiella pneumoniae

    Directory of Open Access Journals (Sweden)

    Edgar Omar Rueda Puente

    2005-01-01

    Full Text Available Una especie vegetal a gran escala se presenta como una alternativa para la producción de ácidos grasos esenciales en nutrición humana por lo que su composición de ácidos grasos cobra especial importancia. Salicornia bigelovii una halófita que se desarrolla en ambientes áridos y costeros como las del estado de Sonora y de la península de Baja California Sur, México, presenta ser una especie alternativa en la producción de aceites comestibles. La fijación de N2 por bacterias asociadas con raíces de Salicornia bigelovii y halófitas similares son una fuente importante de nitrógeno disponible en ecosistemas costeros. La inoculación de Klebsiella pneumoniae y Azospirillum halopraeferens fue evaluada durante el desarrollo vegetativo de Salicornia bigelovii bajo condiciones de campo en dos genotipos (genotipo silvestre y cv. SOS-10, afectando positivamente variables como peso, longitud de plantas, rendimientos de producción y características bioquímicas como proteína total, ceniza, y contenido de lípidos totales en semilla producida. Nuestros resultados sugieren que ambos genotipos de S. bigelovii, bajo las condiciones de campo empleadas, pueden ser mejorados con la aplicación de K. pneumoniae y A. halopraeferens, mostrando una utilidad potencial para productores agrícolas de zonas semiáridas costeras.

  16. Evaluation of human dental loss caused by carbamide peroxide bleacher compared with phosphoric acid conditioning - radioactive method; Avaliacao da perda dental humana com o uso do clareador peroxido de carbamida comparado ao condicionamento com acido fosforico - metodo radiometrico

    Energy Technology Data Exchange (ETDEWEB)

    Adachi, Eduardo Makoto; Yousseff, Michel Nicolau [Sao Paulo Univ., SP (Brazil). Faculdade de Odontologia. Dept. de Dentistica; Saiki, Mitiko [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Lab. de Analise por Ativacao Neutronica

    2002-07-01

    The radiometric method was applied to the evaluation of dental loss caused by carbamide peroxide when it is applied on the surface layers of enamel and dentin tissues. Also the dental loss caused by the etching with 37% phosphoric acid procedure used in aesthetic restoration was assessed for comparison with those results obtained. The tooth samples irradiated with a P standard in a thermal neutron flux of the nuclear reactor were placed in contact with 10% carbamide peroxide or with 37% phosphoric acid solution. The radioactivity of {sup 32} P transferred from the radioactive teeth to the bleaching gel or to etching acid was measured using a Geiger Muller detector to calculate the mass of P removed in this treatment and losses were calculated after obtaining their P concentrations. Results obtained indicated that enamel and dentin exposed to carbamide peroxide bleaching agent lose phosphorus. The extent of enamel loss was smaller than that obtained for dentin. In the case of acid etching, there was no difference between the results obtained for enamel and dentin loss. Also the dentin loss obtained after a treatment of 30 applications of 10% carbamide peroxide was the same magnitude of that one application of 37% phosphoric acid. (author)

  17. Cyclopentenyl acids from sapucainha (Carpotroche brasiliensis Endl, Flacourtiaceae): the first antileprotic used in Brazil; Acidos ciclopentenicos do oleo da sapucainha (Carpotroche brasiliensis Endl, Flacourtiaceae): o primeiro antileprotico usado no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Adriana S.; Lima, Joselia A.; Rezende, Claudia M.; Pinto, Angelo C. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Centro de Tecnologia. Inst. de Quimica]. E-mail: crezende@iq.ufrj.br

    2009-07-01

    The chaoolmugra oil is one of the oldest medicines used for leprosy until the development of sulfas. In Brazil, it is known as sapucainha oil that is obtained from Carpotroche brasilienesis seeds (Flacourtiaceae). Chemical and medicinal studies of sapucainha oil were introduced in Brazil by Peckolt, Cole and Cardoso which showed that major ciclopentenyl fatty acids as chaulmoogric, hydnocarpic and gorlic were responsible for its bactericidal activity. In this work, a method for quantification of sapucainha oil acidic fraction by HRGC was established using methyl n-heptadecanoate as internal standard. The oil chemical stability was investigated by the use of GC-MS, IR, {sup 1}H and {sup 13}C NMR and showed that the main degradation products belong to the cyclopentenyl moiety oxidation. (author)

  18. Effect of the humic acids in the adsorption of U (Vi) in a Mexican natural zeolite; Efecto de los acidos humicos en la adsorcion de U (VI) en una zeolita natural mexicana

    Energy Technology Data Exchange (ETDEWEB)

    Sandoval G, H. A.; Lopez del R, H.; Davila R, J. I.; Mireles G, F., E-mail: hlopezdelrio@hotmail.com [Universidad Autonoma de Zacatecas, Unidad Academica de Estudios Nucleares, Cipres No. 10, Fracc. La Penuela, 98068 Zacatecas (Mexico)

    2012-10-15

    The uranium adsorption was studied in a Mexican natural zeolite of the clinoptilolite type in presence and absence of humic acids (Ha). The adsorption kinetics of the binary U-zeolite and ternary U-Ha-zeolite systems were compared, and the effect of the humic matter impregnated in the zeolite in the uranium removal in function of the solution ph was studied. The experiments were made to environmental conditions and lightly acid ph, with fixed concentrations of uranium and Ha of 1 x 10{sup -5} M and 20 ppm, respectively, and a reason m/V of 5 mg/ml. The uranium adsorption in absence of Ha was of 96% and decreased to 45% in the ternary system U-Ha-zeolite. The data of the binary system U-zeolite were analyzed with three kinetic models of adsorption (pseudo-first order, pseudo-second order and Elovich). By means of non lineal regression was found that the uranium adsorption obeys a kinetic of pseudo-second order. It was observed that the uranium adsorption in the zeolite impregnated with Ha is favored to more ph at 5. (Author)

  19. Synthesis and characterization of chitosan-graft-poly(acrylic acid)/rice husk ash hydrogels composites; Sintese e caracterizacao de hidrogeis compositos de cinza da casca de arroz e quitosana enxertada com poli(acido acrilico)

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigues, Francisco H.A. [Universidade Estadual Vale do Acarau - UVA, Sobral, CE (Brazil); Lopes, Gabriel V.; Pereira, Antonio G.B.; Fajardo, Andre R.; Muniz, Edvani C. [Universidade Estadual de Maringa - UEM, PR (Brazil)

    2011-07-01

    According to environmental concerns, super absorbent hydrogel composites were synthesized based on rice husk ash (RHA), an industrial waste, and Chitosan-graft-poly(acrylic acid). The WAXS and FTIR data confirmed the syntheses of hydrogel composites. The effect of crystalline or amorphous RHA on water uptake was investigated. It was found that the RHA in crystalline form induces higher water capacity (W{sub eq}) of composites hydrogels due to the fact that the intra-interactions among silanol groups on RHA make available new sites in the polymer matrix, which could interact to water. (author)

  20. Preparation and characterization of polymer blends based on recycled PET and polyester derived by terephthalic acid; Preparacao e caracterizacao de blendas polimericas a base de PET reciclado e poliester derivado do acido tereftalico

    Energy Technology Data Exchange (ETDEWEB)

    Ohara, L.; Miranda, C.S.; Fiuza, R.P.; Luporini, S.; Carvalho, R.F.; Jose, N.M., E-mail: leandro.ohara@gmail.co [Universidade Federal da Bahia (GECIM/UFBA), Salvador, BA (Brazil). Inst. de Quimica. Grupo de Energia e Ciencias dos Materiais

    2010-07-01

    Environmentally friendly materials, made from industrial waste, are being increasingly used as a solution to the growing amount of waste generated by society, but also as a cheaper alternative to replace conventional materials for use in construction. In this work were investigated the properties of polymer blends based on recycled PET and a polyester derived from terephthalic acid and glycerin, a co-product of biodiesel. The samples were characterized by XRD, TGA, DSC, FTIR and SEM. The polyester synthesized showed a degradation event near 300 deg C. The blends with higher ratio of PET showed thermal behavior similar to pure PET. The X-ray diffraction showed that the polymer blends are semicrystalline materials. The micrographs presents the presence of a smooth surface, indicating the possibility of miscibility between the arrays. Therefore, the blending makes possible the fabrication of low-cost materials with applications in several areas. (author)

  1. 铌-酒石酸-邻硝基苯基萤光酮-CTMAB 多元络合物显色反应的研究%Colour Reaction of Multicomponent Complex Niobium-Tartaric Acido-Nitrophenylfluorone-CTMAB

    Institute of Scientific and Technical Information of China (English)

    沈含熙; 王连生

    1984-01-01

    @@铌的高灵敏显色反应,迄今报道甚少。以邻硝基苯基萤光酮为显色剂的氯仿萃取光度法[1],虽然具有较高的灵敏度(8=1.38×105),但由于使用有机溶剂进行萃取操作而感不便。我们考虑以胶束增敏反应在水溶液中进行铌的测定来代替萃取光度法,可能使实验操作简化。 试验结果表明,在酒石酸-盐酸介质中,铌(Ⅱ) 可以与邻硝基苯基萤光酮以及阳离子表面活性剂CTMAB形成具有四元组分的络合物,其最大吸收峰位于525nm,相应的摩尔吸光系数可达到1.63×105升·摩尔-1·厘米-1。有可能发展成为水相介质中测定微量铌的高灵敏分光光度法。

  2. Study of stability of humic acids from soil and peat irradiated by gamma rays; Estudo da estabilidade de acidos humicos extraidos de solo e turfa, frente a radiacao ionizante gama

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Wilson Tadeu Lopes da

    1995-07-01

    Humic acids samples (one deriving from a sedimentary soil and other from a peat), in aqueous media, were irradiated with gamma rays, in doses of 10, 50 and 100 kGy, in order to understand their chemical behavior after the irradiation. The material, before and after irradiation, was analyzed by Elemental Analysis, Functional Groups (carboxylic acids and phenols), UV/Vis Spectroscopy (E{sub 4}/E{sub 6} ratio), IR spectroscopy, CO{sub 2} content and Gel permeation Chromatography (GPC) ). The Elemental Analysis showed the humic acid derived from a peat had a most percentage quantity of Carbon and Hydrogen than the material from a sedimentary soil. From the UV/Vis Spectroscopy, it was observed a decrease of E{sub 4}/E{sub 6} ratio with an increase of the applied dose. The data from GPC are in agreement with this. The results showed that the molecular weight of the material increased by exposing it to a larger radiolitical dose. The peat material was less affected by the gamma radiation than the soil material. The carboxylic groups were responsible by radiochemical behavior of the material. (author)

  3. Impregnation of 12-tungstophosphoric acid on silica - part I: determination of impregnation parameters, characterization and evaluation of catalytic activity; Impregnacao do acido 12-fosfotungstico em silica - parte I: determinacao de parametros de impregnacao, caracterizacao e avaliacao da atividade catalitica

    Energy Technology Data Exchange (ETDEWEB)

    Scroccaro, Karine Isabel; Tanobe, Valcineide Oliveira de Andrade; Cocco, Lilian Cristina; Yamamoto, Carlos Itsuo, E-mail: karineisabel@yahoo.com.br [Universidade Federal do Parana, Curitiba, PR (Brazil). Centro Politecnico. Dept. de Engenharia Quimica; Wypych, Fernando [Universidade Federal do Parana, Curitiba, PR (Brazil). Centro Politecnico. Dept. de Quimica

    2012-07-01

    Catalyst based on Kegging-type heteropolyacids (H{sub 3}PW{sub 12}O{sub 40} - HPA), supported on SiO{sub 2} (H{sub 3}PW), were prepared by the impregnation method under different thermal treatment conditions. The materials were characterized by different instrumental techniques and used as catalysts in the methyl esterification reactions of stearic acid. Using the catalyst with 15% of HPA, conversions higher than 60% were obtained after 2 h of reaction at 65 deg C. Recovery studies using hot-filtration with ethanol at 75 deg C showed satisfactory activity for two additional reaction cycles. (author)

  4. Acido tranexâmico e hemostasia em cirurgia de revascularização do miocárdio com circulação extracorpórea Tranexamic acid and hemostasis in myocardial revascularization with extracorporeal circulation

    OpenAIRE

    Guilherme F Vargas; João Nelson R. Branco; Ana Hercília S Guimarães; Cecília Kobata; Espedito T. V. F Silva; Carlos Alberto Teles; Carlos Arnulfo A La Rotta; M. L. A Batista Filho; José Carlos S Andrade; Ênio Buffolo

    1992-01-01

    O antífibrinolítico sintético ácido tranexâmico (Transamin ®) foi avaliado em seus efeitos hemostáticos e poupadores de transfusões homólogas, em pacientes submetidos a revascularização do miocárdio com circulação extracorpórea (CEC). Quarenta pacientes receberam placebo e 55 pacientes foram operados sob o efeito do ácido tranexâmico na dose de 10 g endovenosa no trans-operatório (2 g administrados na indução anestésica e os restantes 8 g nas 4 horas seguintes de cirurgia, de modo contínuo). ...

  5. Poderá o carrapato transmitir a lepra?: isolamento e cultura dum bacilo acido-álcool resistente de sedimento de "Amblyomma cajennense" capturado em leproso: 2ª nota May ticks transmit leprosy?: Second Note

    Directory of Open Access Journals (Sweden)

    H. C. Souza-Araujo

    1942-01-01

    Full Text Available In this 2nd note upon the possibility of transmission of human leprosy by ticks, the A. relates his stepps to obtain the collaboration of his colleagues working in leprosaria in various States of Brazil, Argentina and Paraguay in such studies. Firstly the A. describes the positive results of examination of sediment of ticks, the cattle tick Boophilus microplus (Canestrini, 1888, received from Paraná (Leprosário São Roque , which were put on active lepers, two of them sucking during 9 days and one during 7 days. Two out of three were killed for examination and were very strongly positive for acid-fast bacilli. A series of tubes of Loewenstein medium was smeared with the sediment of such ticks. Secondly the A. relates his personnal experiment, carried out in Rio de Janeiro, trying to infect normal ticks in lepers. The experiment with Boophilus microplus was negative and was twicely positive the experiment with Amblyomma cajennense Fabricius, 1794. The experiment is being in progress and will be continued in other places of Brazil. Finally, after being given the general characteristics of Boophilus microplus, the A. describes the non-chromogenic culture of a acid-fast bacillus isolated by him from sediment of ticks (Amblyomma cajennense captured in lepers from Colônia Santa Isabel (Minas gerais, which parasitism was spontaneous. The first isolation was obtained in Loewenstein medium after 62 days incubation at 37°C. The culture is pure and the bacillus is permanent acid-fast. The plate1, in full color, represents this culture in its four generations. The colonies are pearl-white in color, dry, elevated and rough, developing slowly and beginning as white pinhead points scattered upon the surface of the medium. The culture is not yet rich enough to be inoculated into laboratory animals, which will be done when possible.

  6. Modeling of an immobilized lipase tubular reactor for the production of glycerol and fatty acids from oils; Modelado de un reactor tubular de lipasas inmovilizadas para la produccion de glicerol y acidos grasos a partir de aceites

    Energy Technology Data Exchange (ETDEWEB)

    Oddone, S.; Grasselli, M.; Cuellas, A.

    2010-07-01

    Advances in the design of a bioreactor in the fats and oils industry have permitted the hydrolysis of triglycerides in mild conditions and improved productivity while avoiding the formation of unwanted byproducts. The present work develops a mathematical model that describes the hydrolytic activity of a tubular reactor with immobilized lipases for the production of glycerol and fatty acids from the oil trade. Runge Kuttas numerical method of high order has been applied, considering that there is no accumulation of the substratum in the surface of the membrane, where the enzyme is. At the same time, different equations based on the kinetic model of Michaelis Mentens and the Ping-Pong bi-bi mechanism were examined. Experimental data in discontinuous systems are the basis for the development of the quantitative mathematical model that was used to simulate the process computationally. The obtained results allow for optimizing both the operative variables and the economic aspects of industrial processes. (Author)

  7. Synthesis and reactions with a midines of derivates cycles and of open chain of 2-bromo-3, 4-dioxobutanoic; Sintesis y reacciones con amidinas de derivados ciclicos y de cadena abierta del acido 2-bromo-3, 4-dioxobutanoico

    Energy Technology Data Exchange (ETDEWEB)

    Ancos, B. de; Delgado, F.; Martin, M.R. [Departamento de Quimica Organica, Facultad de Ciencias, Universidad Autonoma, Madrid (Spain)

    1995-12-31

    Bromination of enaminoesters 1 and 5, in appropriate conditions, affords in good yield enamines of the corresponding 2-bromo-3.4-dioxobutanoic acid derivatives 2-3 and 6 or dibromocompounds 4 and 6. The open chain enaminoesters (1-3) are more easily hydrolyzed that enaminofuranones 5 and 6. Prolongated treatment of 6 with hydrochloric acid affords the chlorinated enamine 11. The synthesis of 3-Bromo-4-hydroxy-5-methoxyfuran-2(5H)-one 10 can be achieved by bromination of 12, obtained by hydrolysis of 5. The reaction of methyl 3-bromo-4, 4-dimethoxy-3-oxobutanoate with amidines is a good method for the synthesis of 5-bromo-6-(dimethoxymethyl) pyrimidine-4(3H)-ones substituted at 2 position. Under the same conditions the reaction of furanone 10 with benzamide does not lead to the corresponding imidazoline. (Author) 12 refs.

  8. Effect of glycerin and formic acid in the efficiency of deposit on Zn-Ni, obtained by electrodeposition; Efeito da glicerina e do acido formico na eficiencia de deposito da liga Zn-Ni, obtido atraves de eletrodeposicao

    Energy Technology Data Exchange (ETDEWEB)

    Pedroza, G.A.G.; Souza, C.A.C.; Lima, L.R.P.A.; Ferreira, D.M. [Universidade Federal da Bahia - Escola Politecnica, BA (Brazil)

    2010-07-01

    Additives are added to the electrodeposition of metal coatings to improve the characteristics of the deposit. However, the objective was to investigate the effect of adding glycerin and formic acid in the deposition efficiency and deposit structure of zinc-nickel alloy obtained by electrodeposition. The depositions were made at a galvanostatic current density of 10 mA/cm{sup 2} to obtain a deposit of about 5 mm in thickness. The deposition efficiency was determined through measures of mass, chemical composition of the deposit in the presence and absence of additives was examined by X-ray Spectrometer Fluorescence (XRF) and surface characterization of coatings was performed by Scanning Electron Microscopy (SEM). The high levels of glycerin (0,07 M) and formic acid (0,26 M) in bath deposition increased the deposition efficiency of around 10% to 12% by mass, respectively. (author)

  9. Behavior of uranium isotopes along a tidal cycle in a study affected by acid mine drainage; Comportamiento de los isotopos de uranio a lo largo de un ciclo mareal en un estuario afectado por denaje acido de minas

    Energy Technology Data Exchange (ETDEWEB)

    Hierro, A.; Martin, J. e.; Olias, M.; Garcia, C.; Bolivar, J. P.

    2013-07-01

    The Tinto and Odiel rivers converge in an estuarine system known as the Ria de Huelva, which is an ecosystem of great interest, conditioned by hydrochemical facts. The main objective of this study was to analyze the behavior of uranium isotopes in the waters of the Red River estuary in a tidal cycle under hydrochemical conditions of high gradients of salinity and pH generated by the acidic waters of the Rio Tinto and seawater. (Author)

  10. Labelling of 5-ethyl-5-phenylbarbituric acid with Technetium-99m: biodistribution study in Swiss mice; Marcacao do acido 5-etil-5-fenilbarbiturico com tecnecio-99m: estudo da biodistribuicao em camundongos Swiss

    Energy Technology Data Exchange (ETDEWEB)

    Simoes, Susana B.E.; Oliveira, Marcia B.N. de; Gutfilen, Bianca; Bernardo-Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia. Dept. de Biofisica e Biometria; Alves, Andreia Coelho; Machado-Silva, Jose R. [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia. Dept. de Parasitologia

    1996-07-01

    The 5-ethyl-5-phenylbarbituric acid (phenobarbital) is used as a sedative, hypnotic and anticonvulsant drug. We decided to label it with technetium-99m. In order to determine the optimal conditions, different concentrations of this drug were incubated with various stannous chloride solutions. Then, {sup 99m}Tc was added and chromatography was performed using 0.9% NaCl solution, acetone and n-butyl alcohol as the mobile phase. Using a solution of 0.01 mg/ml stannous chloride and 1.0 mg/ml phenobarbital over 92% of the radioactivity bound to phenobarbital {sup 99m}Tc-phenobarbital. In the biodistribution study, {sup 99m}Tc-phenobarbital was administered in mice intraperitoneal. The main uptake of the labeled drug was in the liver, blood, kidneys, spleen and stomach. The phenobarbital is also used as anesthetic drug in animals. Earlier studies confirm that this drug can dislocate the adult worms of Schistosoma mansoni to mesenteric vein towards the liver and portal vein, so that we used infected animals, radioactivity was not found in isolated worms and we can conclude that the phenobarbital has an indirect action in relation to the displacement of the worms. (author)

  11. Morphology and thermal degradation study of poly(lactic acid)/synthetic mica composites; Estudo da morfologia e da degradacao termica de compositos de poli(acido latico)/mica sintetica organofilica

    Energy Technology Data Exchange (ETDEWEB)

    Souza, D.H.S.; Dias, M.L., E-mail: diegosaboya@ima.ufrj.b [Universidade Federal do Rio de Janeiro (IMA/UFRJ), RJ (Brazil). Inst. de Macromoleculas Professora Eloisa Mano

    2010-07-01

    Poly(lactic acid) (PLA)/synthetic mica composites has been little studied in the literature. In this work, an organophilic synthetic mica was used to prepare PLA nanocomposites. The composites were obtained at an internal mixer containing 3, 5, 7 and 10 wt% of mica. The materials were analyzed by x-ray diffraction, thermogravimetric analysis and gel permeation chromatography. (author)

  12. Resistencia a la insulina inducida por acidos grasos en células de músculo esquelético L6E9: papel de la carnitina palmitoiltransferasa I (CPT-I)

    OpenAIRE

    Sebastián Muñoz, David

    2006-01-01

    La resistencia a la insulina es un estado patológico que se define como la incapacidad del organismo de responder normalmente a las acciones de la insulina. Este estado está ligado a la obesidad, al estilo sedentario de vida y es responsable en gran medida de la aparición de la diabetes de tipo 2. Aunque tradicionalmente el estudio de esta patología se había centrado en el metabolismo de carbohidratos, en las últimas décadas se ha producido un cambio hacia el estudio del metabolismo de ácidos...

  13. Evaluation of the physicochemical properties of structured materials: metallic, polymeric and ceramic, for the treatment of sour gases; Evaluacion de las propiedades fisicoquimicas de materiales estructurados: metalico, polimerico y ceramico, para el tratamiento de gases acidos

    Energy Technology Data Exchange (ETDEWEB)

    Salazar, A.; Chavez, R. H. [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico); Olea, O.; Solis, D., E-mail: rosahilda.chavez@inin.gob.mx [Universidad Autonoma del Estado de Mexico, Facultad de Quimica, Cerro de Coatepec s/n, Ciudad Universitaria, 50100 Toluca, Estado de Mexico (Mexico)

    2013-07-01

    in this work the physicochemical properties of three structured materials: metallic, polymeric and ceramic, from Sulzer Brothers Limited brand, are studied in order to removal sour gases, by absorption process, in aqueous solution of Monoethanolamine (Mea), at 30% weight. Mechanical properties, chemical composition, morphology and corrosion resistance were determined, using different characterization techniques, such as: 1) mechanically, according to standard procedures Astm E-384-1990, 2) chemically, by the corrosion resistance in the presence of an electrochemical cell, in aqueous solution of H{sub 2}SO{sub 4}, 1 N by Astm G-5-1999, 3) morphologically by scanning electron microscopy technique, and 4) efficiency of separation, by the gas chromatography technique in order to determine the chemical absorption of CO{sub 2} by Mea. The ceramic material was the hardest with 700 Hk value and tensile strength of 90 MPa, likewise showed resistance to corrosion of 10.28 m py, separation efficiency of 74% CO{sub 2}, at 10 minutes. The metallic material had a hardness of 190 Hk and it was the most resistant of tension, with 831 MPa, and corrosion resistance of 780.4 x 10{sup -6} m py, likewise promoted CO{sub 2} separation efficiency of 90% during the evaluation. The polymeric material presented hardness of 20 Hk and 35 MPa and it was not suffered surface change with electrochemical attack, with 282.4 x 10{sup -6} m py, and separation efficiency of 88%. Therefore the polymer was the most ductile, with smooth surface and greater resistance with H{sub 2}SO{sub 4}. The metal material was more resistant to plastic deformation and more corrugated surface and the second resistance in the presence of acid medium in aqueous solutions. For all the above, the metallic material is recommended by its greater separation in the reduction of acid gases and the polymer due to its greater chemical resistance. (Author)

  14. FORMULATION D'UN GEL OXYDANT À MATRICE ORGANIQUE APPLICABLE À LA DÉCONTAMINATION NUCLÉAIRE : PROPRIÉTÉS RHÉOLOGIQUES, ACIDO-BASIQUES ET OZONOLYSE DE LA MATRICE

    OpenAIRE

    Rouy, Emmanuel

    2003-01-01

    Un gel fortement acide et oxydant, à matrice purement organique, a été formulé dans l'objectif de l'appliquer sur des parois métalliques contaminées par des radioéléments. Les propriétés rhéologiques pertinentes au regard de l'application envisagée (caractère rhéofluidifiant, thixotropie, seuil d'écoulement...) ont été analysées dans différents milieux : purement aqueux, acide (HNO3 2 mol/kg), acide et cérique ( (NH4)2Ce(NO3)6 1 mol/kg). La matrice organique utilisée, le xanthane, présente po...

  15. Characterization of commercial ceramic adsorbents and their application on naphthenic acids removal of petroleum distillates; Caracterizacao de adsorventes ceramicos comerciais e sua aplicacao na remocao de acidos naftenicos de destilados de petroleo

    Energy Technology Data Exchange (ETDEWEB)

    Silva, J.P.; Senna, L.F. de; Lago, D.C.B. do; Silva Junior, P.F. da; Figueiredo, M.A.G. de; Dias, E.G. [Universidade do Estado do Rio de Janeiro (UERJ), RJ (Brazil)], e-mail: julia_psi@yahoo.com.br; Chiaro, S.S.X. [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil). Centro de Pesquisas (CENPES)

    2006-07-01

    One denominates 'naphthenic acids' to the mixture of carboxylic acids that is present in petroleum oil, and is directly responsible for its acidity and corrosiveness in liquid phase during the refine process. These acids are also presents in distilled fraction of petroleum, causing several problems in final products quality. A possible way to remove them from petroleum distilled fractions is the adsorption in porous materials. However, the published results indicate that ion exchange resins would be the best adsorbents for this process, which would probably increase its cost. In this work, two commercial adsorbents (clay and activated alumina) were characterized by a set of physical-chemistry techniques and evaluated concerning their capacity of removing naphthenic acids from a light petroleum fraction. It was also verified the influence of a previous thermal treatment to the adsorption in their physical-chemistry characteristics and its properties. (author)

  16. Modification of zirconium diphosphate with salicylic acid and its effect on the uranium (Vi) sorption; Modificacion del difosfato de circonio con acido salicilico y su efecto sobre la sorcion de uranio (VI)

    Energy Technology Data Exchange (ETDEWEB)

    Almazan T, M. G.; Garcia G, N. [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico); Simoni, E., E-mail: guadalupe.almazan@inin.gob.mx [Universidad Paris Sud, Instituto de Fisica Nuclear, Georges Clemenceau No. 15, Orsay (France)

    2014-10-15

    The surface of zirconium diphosphate (ZrP{sub 2}O{sub 7}) was modified with salicylic acid and its effect was evaluated on the uranium (Vi) sorption. The modified surface of the material was analyzed with different analytical techniques among which are included the atomic force microscopy, scanning electron microscopy and X-ray photoelectron spectroscopy. This analysis allowed showing that the salicylic acid is being held on the surface of the zirconium diphosphate. The reactivity of modified zirconium diphosphate compared with uranium (Vi) was investigated using the classical method of batch sorption. The analysis of sorption isotherms shows that the salicylic acid has an important effect in the uranium (Vi) sorption. According to the study conducted, the interaction among the uranium (Vi) and the surface of zirconium diphosphate modified with the salicylic acid most likely leads to the complexes formation of binary (U(Vi)/ZrP{sub 2}O{sub 7}) and ternary (U(Vi)/salicylate/ZrP{sub 2}O{sub 7}) surface. (Author)

  17. Influence of the temperature in the uranyl sorption in zirconium diphosphate modified with salicylic acid; Influencia de la temperatura en la sorcion de uranilo en difosfato de circonio modificado con acido salicilico

    Energy Technology Data Exchange (ETDEWEB)

    Garcia G, N.; Solis C, D. A. [Universidad Autonoma del Estado de Mexico, Facultad de Quimica, Paseo Colon y Paseo Tollocan s/n, 50000 Toluca, Estado de Mexico (Mexico); Ordonez R, E., E-mail: nidgg@yahoo.com.mx [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (MX)

    2011-11-15

    In this work the experimental conditions were established to evaluate the uranium (Vi) sorption to 20 and 40 C on the surface of the zirconium diphosphate (ZrP{sub 2}O{sub 7}) modified with a solution of salicylic acid 0.1 M. The modification of the ZrP{sub 2}O{sub 7} was produced during the hydrate process, taking advantage that these are formed complexes between the carboxyl and hydroxyl groups of salicylic acid and amphoteric species of the interface solid/liquid. The method is used by lots to elaborate the isotherms that explain the behavior of this sorption in different ph conditions and temperature, the quantity of the uranium reaction is analyzed with the fluorescence technique. The results indicated that in the temperature increases the uranium sorption on the material and is more efficient to low ph values. (Author)

  18. Dietary DHA reduced downstream endocannabinoid and inflammatory gene expression, epididymal fat mass, and improved aspects of glucose use in muscle in C57BL/6J mice

    Science.gov (United States)

    Objective: Endocannabinoid system (ECS) overactivation is associated with increased adiposity and likely contributes to type II diabetes risk. Elevated tissue cannabinoid receptor 1 (CB1) and circulating endocannabinoids derived from the n-6 polyunsaturated acid (PUFA) arachidonic acid occur in obes...

  19. Tumor growth reduction is regulated at the gene level in Walker 256 tumor-bearing rats supplemented with fish oil rich in EPA and DHA

    Energy Technology Data Exchange (ETDEWEB)

    Borghetti, G.; Yamazaki, R.K.; Coelho, I.; Pequito, D.C.T.; Schiessel, D.L.; Kryczyk, M.; Mamus, R.; Naliwaiko, K.; Fernandes, L.C. [Departamento de Fisiologia, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, PR (Brazil)

    2013-08-23

    We investigated the effect of fish oil (FO) supplementation on tumor growth, cyclooxygenase 2 (COX-2), peroxisome proliferator-activated receptor gamma (PPARγ), and RelA gene and protein expression in Walker 256 tumor-bearing rats. Male Wistar rats (70 days old) were fed with regular chow (group W) or chow supplemented with 1 g/kg body weight FO daily (group WFO) until they reached 100 days of age. Both groups were then inoculated with a suspension of Walker 256 ascitic tumor cells (3×10{sup 7} cells/mL). After 14 days the rats were killed, total RNA was isolated from the tumor tissue, and relative mRNA expression was measured using the 2{sup -ΔΔCT} method. FO significantly decreased tumor growth (W=13.18±1.58 vs WFO=5.40±0.88 g, P<0.05). FO supplementation also resulted in a significant decrease in COX-2 (W=100.1±1.62 vs WFO=59.39±5.53, P<0.001) and PPARγ (W=100.4±1.04 vs WFO=88.22±1.46, P<0.05) protein expression. Relative mRNA expression was W=1.06±0.022 vs WFO=0.31±0.04 (P<0.001) for COX-2, W=1.08±0.02 vs WFO=0.52±0.08 (P<0.001) for PPARγ, and W=1.04±0.02 vs WFO=0.82±0.04 (P<0.05) for RelA. FO reduced tumor growth by attenuating inflammatory gene expression associated with carcinogenesis.

  20. Early infant diet and the omega 3 fatty acid DHA: effects on resting cardiovascular activity and behavioral development during the first half-year of life

    Science.gov (United States)

    The course of postnatal maturation of cardiac control has implications for cognitive development, but the influence of early nutrition on underlying processes is still being defined. This investigation evaluated the effects of different diets on interactions among these variables during the first ha...

  1. Tumor growth reduction is regulated at the gene level in Walker 256 tumor-bearing rats supplemented with fish oil rich in EPA and DHA.

    Science.gov (United States)

    Borghetti, G; Yamazaki, R K; Coelho, I; Pequito, D C T; Schiessel, D L; Kryczyk, M; Mamus, R; Naliwaiko, K; Fernandes, L C

    2013-08-01

    We investigated the effect of fish oil (FO) supplementation on tumor growth, cyclooxygenase 2 (COX-2), peroxisome proliferator-activated receptor gamma (PPARγ), and RelA gene and protein expression in Walker 256 tumor-bearing rats. Male Wistar rats (70 days old) were fed with regular chow (group W) or chow supplemented with 1 g/kg body weight FO daily (group WFO) until they reached 100 days of age. Both groups were then inoculated with a suspension of Walker 256 ascitic tumor cells (3 × 10(7) cells/mL). After 14 days the rats were killed, total RNA was isolated from the tumor tissue, and relative mRNA expression was measured using the 2(-ΔΔCT) method. FO significantly decreased tumor growth (W=13.18 ± 1.58 vs WFO=5.40 ± 0.88 g, Psupplementation also resulted in a significant decrease in COX-2 (W=100.1 ± 1.62 vs WFO=59.39 ± 5.53, Pprotein expression. Relative mRNA expression was W=1.06 ± 0.022 vs WFO=0.31 ± 0.04 (P<0.001) for COX-2, W=1.08 ± 0.02 vs WFO=0.52 ± 0.08 (P<0.001) for PPARγ, and W=1.04 ± 0.02 vs WFO=0.82 ± 0.04 (P<0.05) for RelA. FO reduced tumor growth by attenuating inflammatory gene expression associated with carcinogenesis.

  2. Pro-resolving mediators produced from EPA and DHA: Overview of the pathways involved and their mechanisms in metabolic syndrome and related liver diseases.

    Science.gov (United States)

    López-Vicario, Cristina; Rius, Bibiana; Alcaraz-Quiles, José; García-Alonso, Verónica; Lopategi, Aritz; Titos, Esther; Clària, Joan

    2016-08-15

    A novel genus of pro-resolving lipid mediators endogenously generated from omega-3 polyunsaturated fatty acids has been identified in exudates obtained during the resolution phase of acute inflammation. The term specialized pro-resolving mediators (SPM) has been coined for these lipid mediators, comprising four novel chemical mediator families designated resolvins of the E series (if derived from eicosapentaenoic acid) and resolvins of the D series, protectins and maresins (if generated from docosahexaenoic acid). These SPM act not only as "stop-signals" of inflammatory response, but also as facilitators of the ability of macrophages to clear apoptotic cells (efferocytosis) and migrate to peripheral lymph nodes (efflux), thus, expediting their removal from sites of inflammation. In this review, we provide an overview of the current efforts to elucidate the structure-function, biosynthesis and actions of these omega-3-derived SPM in the context of inflammatory diseases. We specifically highlight the role of these SPM as endogenous counter-regulators of the persistent inflammatory status present in adipose tissue of obese individuals and describe the potential therapeutic impact of these bioactive lipid autacoids on the prevention of hepatic co-morbidities associated with obesity and the metabolic syndrome. PMID:25987424

  3. DHA和EPA的生理功能及开发前景%Physiological Functions and Utilizing Prospect of DHA and EPA

    Institute of Scientific and Technical Information of China (English)

    李春香; 刘永杰

    2002-01-01

    DHA和EPA均为ω-3高碳不饱和脂肪酸,在健脑增智、防止心血管疾病、降血脂防动脉硬化、保护视力等方面有重要的作用,是人体健康必要的脂肪酸.将DHA和EPA应用于食品、药品及动物饲料具有广泛的前景.但是要合理食用DHA、EPA,防止其氧化和过量.

  4. MFA of omega-3 fatty acids EPA & DHA from a Norwegian resource perspective: Implications for future growth in fisheries and aquaculture toward 2050

    OpenAIRE

    Gracey, Erik

    2014-01-01

    Much of the current research into the sustainability of the fisheries and aquaculture industry has focused on achieving continued growth. The report “Value creation from productive oceans,” makes annual growth predictions of 4% for aquaculture and 7% for the marine ingredients sector from 2010 to 2050. The Norwegian government incorporated these growth rates into a political vision presented to parliament as Meld.St.22 “the world’s foremost seafood nation.” Among many...

  5. 真菌发酵生产EPA及DHA影响因素的研究进展%Factors affect the Production of EPA and DHA by Fungal Fermentation

    Institute of Scientific and Technical Information of China (English)

    陈欣

    2002-01-01

    对真菌发酵生产EPA及DHA的影响因素进行综述,介绍了菌种、碳源、氮源、C/N比、pH值、温度、发酵时间、通气量、代谢途径的调控、种龄和接种量等因素对EPA及DHA产量的影响.

  6. TECHNOLOGY OF PURIFICATION OF EPA、DHA WITH DEEP PROCESSING OF FISH OIL%鱼油深加工提纯EPA、DHA工艺技术

    Institute of Scientific and Technical Information of China (English)

    宋海林; 金荣成; 李先慧

    1999-01-01

    @@ 1 前言 随着人们对深海鱼油的广泛重视,EPA和DHA对人体的医疗保健作用也越来越倍受关注.国内外已研制出多种制备方法和多种鱼油产品,但都因提取时间长、成本高、操作复杂、工艺条件不易控制以及EPA、DHA易发生异构化而受到限制,难于实现工业化生产.本法制备的EPA和DHA不但纯度高、色泽好,而且易于生产实践.

  7. DHA的功能及在食品添加中的应用研究%Functions of DHA and Its Application in Food Addictives

    Institute of Scientific and Technical Information of China (English)

    黄菊华; 李蕴成

    2014-01-01

    DHA是二十二碳六烯酸(Docoseahexaenoic acid)的简称,为人体必需的ω-3系长链多不饱和脂肪酸.DHA对人体有重要作用,能促进婴幼儿生长发育,防治成人心血管疾病,延缓衰老、预防老年痴,保护视网膜、改善视力,抗癌、抗炎症等.近年来,随着人们保健意识的增强,DHA在食品添加中的研究与应用也更广泛和深入.目前,我国人群的DHA摄入量不足,及时补充足量DHA很有必要.

  8. Enhanced apoptotic effects of dihydroartemisinin-aggregated gelatin and hyaluronan nanoparticles on human lung cancer cells.

    Science.gov (United States)

    Sun, Qian; Teong, Benjamin; Chen, I-Fen; Chang, Shwu Jen; Gao, Jimin; Kuo, Shyh-Ming

    2014-04-01

    Recent studies suggest that dihydroartemisinin (DHA), a derivative of artemisinin isolated from the traditional Chinese herb Artemisia annua L., has anticancer properties. Due to poor water solubility, poor oral activity, and a short plasma half-life, large doses of DHA have to be injected to achieve the necessary bioavailability. This study examined increasing DHA bioavailability by encapsulating DHA within gelatin (GEL) or hyaluronan (HA) nanoparticles via an electrostatic field system. Observations from transmission electron microscopy show that DHA in GEL and HA nanoparticles formed GEL/DHA and HA/DHA aggregates that were approximately 30-40 nm in diameter. The entrapment efficiencies for DHA were approximately 13 and 35% for the GEL/DHA and HA/DHA aggregates, respectively. The proliferation of A549 cells was inhibited by the GEL/DHA and HA/DHA aggregates. Fluorescent annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) staining displayed low background staining with annexin V-FITC or PI on DHA-untreated cells. In contrast, annexin V-FITC and PI stains dramatically increased when the cells were incubated with GEL/DHA and HA/DHA aggregates. These results suggest that DHA-aggregated GEL and HA nanoparticles exhibit higher anticancer proliferation activities than DHA alone in A549 cells most likely due to the greater aqueous dispersion after hydrophilic GEL or HA nanoparticles aggregation. These results demonstrate that DHA can aggregate with nanoparticles in an electrostatic field environment to form DHA nanosized aggregates. PMID:24039154

  9. An investigation of the auto-induction of and gender-related variability in the pharmacokinetics of dihydroartemisinin in the rat

    Directory of Open Access Journals (Sweden)

    Zhu Fanping

    2012-11-01

    Full Text Available Abstract Background Artemisinin (QHS and its derivatives dihydroartemisinin (DHA, artemether and artesunate have become the first-line anti-malarials in areas of multidrug resistance. Declining plasma concentrations during the repeated dosing have been reported for QHS, artemether and less convincingly for artesunate (ARS. However, there is limited information on whether the concentrations of their active metabolite DHA and its subsequent metabolites increased after multiple drug administrations. This study was designed to evaluate the potential auto-induction metabolism of DHA in animal species. The sex-specific effect on the pharmacokinetic profiles of DHA and its metabolites was studied. The pharmacokinetics of ARS, the prodrug of DHA, and its phase I/II metabolites were also investigated. Methods Two groups of rats received a single oral dose of DHA or ARS, and another two groups of rats were given oral doses of DHA or ARS once daily for five consecutive days. Plasma samples were analyzed for DHA, ARS and their phase I/II metabolites, using a validated liquid chromatography tandem mass spectrometric (LC-MS method. Results DHA, monohydroxylated DHA (M1 and the glucuronide of DHA (DHA-G were detected in rat plasma after oral administration of DHA or ARS. Neither DHA nor its metabolites (M1 and DHA-G changed significantly (P > 0.05 in AUC0-t after 5-day oral doses of DHA or ARS. Sex difference was observed for DHA and its metabolites (M1 and DHA-G, whereas its prodrug ARS did not show similar characteristics for the corresponding metabolites (DHA, M1 and DHA-G. Conclusions The results gave the direct evidence for the absence of auto-induction of phase I and phase II metabolism of DHA and ARS in rats. The sex effect existed for DHA but not for ARS, which could be caused by the sex-specific differences in absorption of DHA.

  10. Composti perfluorurati: Valutazione degli effetti biologici e molecolari in modelli cellulari

    OpenAIRE

    Pirini, Francesca

    2014-01-01

    L’acido perfluorottanoico (PFOA) e l’acido perfluoronanoico (PFNA) sono composti perfluorurati (PFCs) comunemente utilizzati nell’industria, negli ultimi 60 anni, per diverse applicazioni. A causa della loro resistenza alla degradazione, questi composti sono in grado di accumularsi nell’ambiente e negli organismi viventi, da cui possono essere assunti in particolare attraverso la dieta. Le esistenti evidenze sugli effetti dell’esposizione negli animali, tra cui la potenziale cancerogenicità, ...

  11. Ácido graxo a-linolênico causa o mesmo efeito que seus derivados de cadeia longa em ratos portadores de tumor de Walker 256?

    OpenAIRE

    Schiessel, Dalton Luiz

    2012-01-01

    Resumo: Alteracoes na dieta estabelecidas nos ultimos 200 anos causaram reducao no consumo de acidos graxos poliinsaturados (AGPI) n-3, aumento do consumo de gordura total, saturada e rica em AGPI n-6. Estas mudancas foram fatores determinantes para o aumento da incidencia de doencas cronicas nao transmissiveis entre estas o cancer. Estima-se que um terco de todos os canceres pode ser prevenido pelo estilo de vida saudavel e dieta saudavel. AGPI n-3, em especial o acido graxo eicosapentaenoic...

  12. Phosphategypsum wastes in Venice lagoon. Radiological impact; Le discariche di fosfogessi nella laguna di Venezia. Valutazioni preliminari dell'impatto radiologico

    Energy Technology Data Exchange (ETDEWEB)

    Belli, M; Blasi, M; Guogang, J.; Rosamilia, S.; Sansone, U. [Agenzia Nazionale per la Protezione dell' Ambiente, Rome (Italy); Biancotto, R.; Bidoli, P.; Sepulcri, D. [Agenzia Regionale di Prevenzione e Protezione del Veneto, Venice (Italy). Dipt. provinciale di Venezia; Cavolo, F. [Smilax, Mira, VE (Italy)

    2000-07-01

    The phosphoric minerals utilized in phosphoric acid production, presents high concentrations of radioactive materials: U238, Th 232, K 40. The phosphogypsum is the waste material obtained in the phosphoric acid production in wet process. This type of production method is employed for many years in Venice lagoon (Porto Marghera chemical plants). In this paper are reported evaluations of radiological impact on aquatic environment of lagoon. [Italian] Con il termine di fosfogessi si intende comunemente il materiale di risulta che si ottiene nella produzione di acido fosforico attraverso la via umida (attacco acido). Questa tipologia di produzione che ha operato per diversi decenni a Porto Marghera, e' finalizzata allo scopo di ottenere acido fosforico principalmente per l'industria dei fertilizzanti e quindi come prodotto intermedio per la chimica e per le preparazioni alimentari. Il fosforo, elemento principale della reazione, era ricavato da rocce fosfatiche di origine sedimentaria marina provenienti per lo piu' dall'Africa settentrionale. Il sistema produttivo utilizzato negli impianti di Porto Marghera era basato su una reazione principale, che partendo dal minerale attraverso un attacco acido, produceva acido fosforico: Ca{sub 3}(PO{sub 4}){sub 2} (Minerale Fosforico) + 3H{sub 2}SO{sub 4} (Acido Solforico) + 3H{sub 2}O (Acqua) {yields} 2H{sub 3}PO{sub 4} (Acido fosforico) + 3CaSO{sub 4}H{sub 2}O (Solfato di calcio (gesso)). In particolare il minerale era preventivamente macinato e vagliato, quindi si procedeva alla sua miscelazione con l'acido fosforico ed alla successiva reazione del composto ottenuto.

  13. Alessandri_Buffer_rev1.xls

    OpenAIRE

    S. Alessandri

    2006-01-01

    Applicazione Excel per la simulazione delle caratteristiche chimiche di una miscela acquosa composta da un acido debole, un suo sale, un acido forte ed una base forte. L'applicazione produce risultati ottenuti con tre livelli di approssimazione, documentati e descritti, e la soluzione esatta degli equilibri in gioco. Il calcolo del pH all'equilibrio viene restituito in forma di piaccametro simulato.

  14. Bacteria obtained from a sequencing batch reactor that are capable of growth on dehydroabietic acid.

    Science.gov (United States)

    Mohn, W W

    1995-06-01

    Eleven isolates capable of growth on the resin acid dehydroabietic acid (DhA) were obtained from a sequencing batch reactor designed to treat a high-strength process stream from a paper mill. The isolates belonged to two groups, represented by strains DhA-33 and DhA-35, which were characterized. In the bioreactor, bacteria like DhA-35 were more abundant than those like DhA-33. The population in the bioreactor of organisms capable of growth on DhA was estimated to be 1.1 x 10(6) propagules per ml, based on a most-probable-number determination. Analysis of small-subunit rRNA partial sequences indicated that DhA-33 was most closely related to Sphingomonas yanoikuyae (Sab = 0.875) and that DhA-35 was most closely related to Zoogloea ramigera (Sab = 0.849). Both isolates additionally grew on other abietanes, i.e., abietic and palustric acids, but not on the pimaranes, pimaric and isopimaric acids. For DhA-33 and DhA-35 with DhA as the sole organic substrate, doubling times were 2.7 and 2.2 h, respectively, and growth yields were 0.30 and 0.25 g of protein per g of DhA, respectively. Glucose as a cosubstrate stimulated growth of DhA-33 on DhA and stimulated DhA degradation by the culture. Pyruvate as a cosubstrate did not stimulate growth of DhA-35 on DhA and reduced the specific rate of DhA degradation of the culture. DhA induced DhA and abietic acid degradation activities in both strains, and these activities were heat labile. Cell suspensions of both strains consumed DhA at a rate of 6 mumol mg of protein-1 h-1.(ABSTRACT TRUNCATED AT 250 WORDS)

  15. The influence of supplemental docosahexaenoic and arachidonic acids during pregnancy and lactation on neurodevelopment at eighteen months

    NARCIS (Netherlands)

    van Goor, Saskia A.; Dijck-Brouwer, D. A. Janneke; Erwich, Jan Jaap H. M.; Schaafsma, Anne; Hadders-Algra, Mijna

    2011-01-01

    Docosahexaenoic acid (DHA) and arachidonic acid (AA) are important for neurodevelopment. The effects of DHA (220 mg/day, n=41), DHA+AA (220 mg/day, n=39) or placebo (n=34) during pregnancy and lactation on neurodevelopment at 18 months, and the relations between umbilical cord DHA, AA and Mead acid

  16. ENRIQUECIMIENTO DE HUEVOS CON ÁCIDOS GRASOS OMEGA-3 MEDIANTE LA SUPLEMENTACIÓN CON SEMILLA DE LINO (Linum usitatissimum EN LA DIETA

    Directory of Open Access Journals (Sweden)

    Gonzalo Díaz

    2009-04-01

    Full Text Available Objetivo. Enriquecer los huevos de gallina con ácidos grasos omega-3 (n-3 mediante la inclusión de semillas de lino en la dieta. Materiales y métodos. La dieta de las aves fue formulada con niveles de 0 (L0, 10 (L10, 15 (L15 y 20% (L20 de linaza. Se determinó la composición de ácidos grasos por cromatografía de gases y se evaluó la producción y calidad del huevo. Resultados. El porcentaje de producción de huevo fue superior en los grupos L0 y L15 (p<0.05 con 93,0 y 91,5% de producción comparada con 86,0 % en los grupos L10 y L20. La mejor conversión de alimento se presentó también en los grupos L0 y L15. Con la inclusión de semilla de lino, se incrementó el contenido de ácidos grasos n-3 desde 3,0 % en el grupo L0 hasta 12,6 % en el grupo L20, siendo los ácidos grasos n-3 á-linolénico (C18:3, n-3 y docosahexahenóico (DHA, C22:6, n-3 los hallados en mayor concentración. Con la inclusión de linaza en la dieta de las gallinas ponedoras se redujo el contenido de acido linoleico (C18:2n-6, lo cual resultó en una disminución en la relación n-6: n-3. Conclusiones. Con base en la evaluación económica se concluye que es posible enriquecer la yema de huevo de gallina hasta con un 10,1 % de ácidos grasos n-3 mediante la inclusión de un 15 % de linaza en la dieta de las gallinas, sin embargo, esto representó un sobrecosto de producción del 12%.

  17. Differential effects of eicosapentaenoic acid and docosahexaenoic acid in promoting the differentiation of 3T3-L1 preadipocytes.

    Science.gov (United States)

    Murali, Ganesan; Desouza, Cyrus V; Clevenger, Michelle E; Ramalingam, Ramesh; Saraswathi, Viswanathan

    2014-01-01

    The objective of this study was to determine the effects of enrichment with n-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on the differentiation of 3T3-L1 preadipocytes. Enrichment with DHA but not EPA significantly increased the differentiation markers compared to control differentiated cells. DHA compared to EPA treatment led to a greater increase in adiponectin secretion and, conditioned media collected from DHA treated cells inhibited monocyte migration. Moreover, DHA treatment resulted in inhibition of pro-inflammatory signaling pathways. DHA treated cells predominantly accumulated DHA in phospholipids whereas EPA treatment led to accumulation of both EPA and its elongation product docosapentaenoic acid (DPA), an n-3 fatty acid. Of note, adding DPA to DHA inhibited DHA-induced differentiation. The differential effects of EPA and DHA on preadipocyte differentiation may be due, in part, to differences in their intracellular modification which could impact the type of n-3 fatty acids incorporated into the cells.

  18. The Effect of Omega-3 Docosahexaenoic Acid Supplementation on Gestational Length: Randomized Trial of Supplementation Compared to Nutrition Education for Increasing n-3 Intake from Foods

    OpenAIRE

    Harris, Mary A.; Reece, Melanie S.; McGregor, James A; Wilson, John W; Shannon M. Burke; Marsha Wheeler; Anderson, Jennifer E.; Garry W. Auld; French, Janice I.; Allen, Kenneth G. D.

    2015-01-01

    Objective. DHA supplementation was compared to nutrition education to increase DHA consumption from fish and DHA fortified foods. Design. This two-part intervention included a randomized double-blind placebo controlled DHA supplementation arm and a nutrition education arm designed to increase intake of DHA from dietary sources by 300 mg per day. Setting. Denver Health Hospitals and Clinics, Denver, Colorado, USA. Population. 871 pregnant women aged 18–40 were recruited between16 and 20 weeks ...

  19. Short term effects of different omega-3 fatty acid formulation on lipid metabolism in mice fed high or low fat diet

    Directory of Open Access Journals (Sweden)

    Tang Xiao

    2012-07-01

    Full Text Available Abstract Background Bioactivities of Docosahexaenoic acid (DHA and Eicosapentaenoic acid (EPA depend on their chemical forms. The present study was to investigate short term effects of triglyceride (TG, ethyl ester (EE, free fatty acid (FFA and phospholipid (PL forms of omega-3 fatty acid (FA on lipid metabolism in mice, fed high fat or low fat diet. Method Male Balb/c mice were fed with 0.7% different Omega-3 fatty acid formulation: DHA bound free fatty acid (DHA-FFA, DHA bound triglyceride (DHA-TG, DHA bound ethyl ester (DHA-EE and DHA bound phospholipid (DHA-PL for 1 week, with dietary fat levels at 5% and 22.5%. Serum and hepatic lipid concentrations were analyzed, as well as the fatty acid composition of liver and brain. Result At low fat level, serum total cholesterol (TC level in mice fed diets with DHA-FFA, DHA-EE and DHA-PL were significantly lower than that in the control group (P P  0.05, DHA-EE (P  0.05 and DHA-PL (P P P P P P  Conclusion The present study suggested that not only total dietary fat content but also the molecular forms of omega-3 fatty acids contributed to lipid metabolism in mice. DHA-PL showed effective bioactivity in decreasing hepatic and serum TC, TG levels and increasing omega-3 concentration in liver and brain.

  20. 利用变性淀粉微胶囊化微藻单细胞DHA油脂的研究%Study on Modified Starch Microencapsulation of DHA Single Cell Oil from Microalgae

    Institute of Scientific and Technical Information of China (English)

    吴克刚; 孟宏昌

    2004-01-01

    本文研究变性淀粉作为壁材对DHASCO的微胶囊包埋效果及其氧化稳定性.实验结果表明,变性淀粉是一种优良壁材,能有效地包埋DHASCO;特别是National变性淀粉包埋的DHASCO,其表面油和溶剂浸出率最低,包埋率最高,室温存放6个月DHA仅降低3.26%;不过,与阿拉伯胶比较,变性淀粉的氧化稳定性较差.

  1. Optimización del enriquecimiento de nauplios de Artemia mediante el uso de emulsiones lipídicas formuladas a partir de aceites sintéticos ricos en DHA

    OpenAIRE

    Viciano Delibano, Elena

    2015-01-01

    Los nauplios de Artemia se utilizan como presa viva en criaderos de organimos marinos de todo el mundo debido a su disponibilidad y digestibilidad, pero su valor nutricional no se ajusta a las demandas o necesidades de las larvas de peces, moluscos o crustáceos marinos, ya que carecen de los ácidos grasos esenciales (EFA, Essential Fatty Acids) para estos organismos. Por tanto, es indispensable llevar a cabo un enriquecimiento de los nauplios de Artemia. El enriquecimiento consiste en incubac...

  2. The Study of Adsorption of EPA and DHA from Fish Oil Using Molecular Sieve%分子筛吸附鱼油中DHA与EPA特性的研究

    Institute of Scientific and Technical Information of China (English)

    郝六平; 邓荣辉; 杜会茹

    2008-01-01

    实验改进了硝酸银修饰分子筛的方法.研究了修饰分子筛对EPA乙酯和DHA乙酯的吸附特性.结果表明,分子筛对EPA乙酯和DHA乙酯的吸附属于单分子层吸附.修饰分子筛在40℃时对EPA乙酯和DHA乙酯的最大吸附量分别为37.4 mg/g和24.2 mg/g.

  3. 硝酸银-水法提纯高含量DHA和EPA的实验研究%Study on extracting high contents of DHA and EPA by using silver nitrate-water method

    Institute of Scientific and Technical Information of China (English)

    陶遵威; 解红武; 刘惠芝

    2004-01-01

    目的从鱼油中分离提纯高含量的DHA和EPA方法用硝酸银-水法对鱼油乙酯化产品进行分离提纯.结果用硝酸银-水法分离粗鱼油得到(1)DHA、EPA总含量大于95%的产品;(2)DHA含量大于95%的产品;(3)EPA含量高于DHA的产品.结论研究的工艺可降低生产高含量鱼油DHA、EPA的成本,降低现售DHA、EPA产品的副作用,提高DHA、EPA产品的稳定性.

  4. Draft Genome Sequence of Klebsiella pneumoniae KGM-IMP216 Harboring bla CTX-M-15, bla DHA-1, bla TEM-1B, bla NDM-1, bla SHV-28, and bla OXA-1, Isolated from a Patient in Lebanon

    OpenAIRE

    Tokajian, Sima; Eisen, Jonathan A.; Jospin, Guillaume; Matar, Ghassan; Araj, George F.; Coil, David A.

    2016-01-01

    We present the draft genome of highly drug-resistant Klebsiella pneumoniae KGM-IMP216, isolated from a urine sample collected from a patient in Lebanon. The draft genome sequence consisted of 77 contigs, including a combined 5,731,500 bases with 57% G+C content.

  5. Continuous gradient temperature Raman spectroscopy of the long chain polyunsaturated fatty acids Docosapentaenoic (DPA, 22:5n-6) and Docosahexaenoic (DHA; 22:6n-3) from -100 to 20° C

    Science.gov (United States)

    The structural, cognitive and visual development of the human brain and retina strictly require long-chain polyunsaturated fatty acids (LC-PUFA). Excluding water, the mammalian brain is about 60% lipid. One of the great unanswered questions with respect to biological science in general is the absolu...

  6. Use of probabilistic safety analysis for design of emergency mitigation systems in hydrogen producer plant with sulfur-iodine technology, Section II: sulfuric acid decomposition; Uso de analisis probabilistico de seguridad para el diseno de sistemas de mitigacion de emergencia en planta productora de hidrogeno con tecnologia azufre-iodo, Seccion II: descomposicion de acido sulfurico

    Energy Technology Data Exchange (ETDEWEB)

    Mendoza A, A.; Nelson E, P. F.; Francois L, J. L. [Facultad de Ingenieria, Departamento de Sistemas Energeticos, UNAM, Paseo Cuauhnahuac 8532, 62550 Jiutepec, Morelos (Mexico)], e-mail: iqalexmdz@yahoo.com.mx

    2009-10-15

    Over the last decades, the need to reduce emissions of greenhouse gases has prompted the development of technologies for the production of clean fuels through the use of primary energy resources of zero emissions, as the heat of nuclear reactors of high temperature. Within these technologies, one of the most promising is the hydrogen production by sulfur-iodine cycle coupled to a high temperature reactor initially proposed by General Atomics. By their nature and because it will be large-scale plants, the development of these technologies from its present phase to its procurement and construction, will have to incorporate emergency mitigation systems in all its parts and interconnections to prevent undesired events that could put threaten the plant integrity and the nearby area. For the particular case of sulfur-iodine thermochemical cycle, most analysis have focused on hydrogen explosions and failures in the primary cooling systems. While these events are the most catastrophic, is that there are also many other events that even taking less direct consequences, could jeopardize the plant operation, the people safety of nearby communities and carry the same economic consequences. In this study we analyzed one of these events, which is the formation of a toxic cloud prompted by uncontrolled leakage of concentrated sulfuric acid in the second section of sulfur-iodine process of General Atomics. In this section, the sulfuric acid concentration is near to 90% in conditions of high temperature and positive pressure. Under these conditions the sulfuric acid and sulfur oxides from the reactor will form a toxic cloud that the have contact with the plant personnel could cause fatalities, or to reach a town would cause suffocation, respiratory problems and eye irritation. The methodology used for this study is the supported design in probabilistic safety analysis. Mitigation systems were postulated based on the isolation of a possible leak, the neutralization of a pond of sulfuric acid and finally washing the same; later some scenarios and modifications were analyzed to determine their impact on the likelihood of failure system and provide feedback design with the information produced in the probabilistic safety analysis. (Author)

  7. The speciation of products from the reaction of {sup 51} Cr(VI) with mineral acids by high efficiency liquid chromatography; Especiacao dos produtos da reacao entre {sup 51} Cr(VI) e acidos minerais por cromatografia liquida de alta eficiencia

    Energy Technology Data Exchange (ETDEWEB)

    Pezzin, Sergio H.; Collins, Carol H.; Collins, Kenneth E. [Universidade Estadual de Campinas, SP (Brazil). Inst. de Quimica; Archundia, Cielita [Universidad Nacional Autonoma de Mexico, Mexico DF (Mexico). Inst. de Ciencias Nucleares

    1996-07-01

    The speciation of the products from the reaction of {sup 51} Cr(VI) with concentrated acids (HClO{sub 4}, HCl e HF) was carried out by ion chromatography. The separation was made on a Partisil SCX (10 {mu}m) column, using HClO{sub 4} and Ca(ClO{sub 4}){sub 2} solutions as eluents. The eluates were measured by {gamma}-counting. The results for the reaction (1h) of carrier-free {sup 51} Cr(VI) with HCl show that 96.4% from the {sup 51} Cr is not retained on the column (anionic and/or neutral species). A similar result (92.7% not retained) is observed in a Cr(VI)-HF system. However, for the reaction with HClO{sub 4}, 82% of the {sup 51} Cr was observed as a +3 species and only 5.9% as anionic and/or neutral species. (author)

  8. Synthesis of zeolites coal ash in surfactant modified in application and removal of orange 8 acid solution: study in batch, fixed bed column and evaluation ecotoxicological; Sintese de zeolitas de cinzas de carvao modificada por surfactante e aplicacao na remocao de acido laranja 8 de solucao aquosa: estudo em leito movel, coluna de leito fixo e avaliacao ecotoxicologica

    Energy Technology Data Exchange (ETDEWEB)

    Magdalena, Carina Pitwak

    2015-09-01

    In this study, synthesized zeolitic material from coal ash and modified cationic surfactant was used for removing the acid dye Orange 8 (AL8) by adsorption process using moving bed and fixed-bed column. The raw material and adsorbents were characterized by different techniques, such as X-ray diffraction, X-ray fluorescence spectroscopy, among others. The adsorption of AL8 was performed by moving bed in order to optimize the results when they are launched in a fixed bed. The effects of adsorption on zeolite AL8 were compared: (1) Effect of counterions Br{sup -} and Cl{sup -} surfactant used in the modification of the zeolite; (2) effect of type of coal ash used as raw material in the synthesis of zeolites (fly and bottom). The following adsorbents were used in the study: fly and bottom zeolite modified by surfactant hexadecyltrimethylammonium bromide (ZLMS-Br-Br and ZPMS-Br) and fly zeolite modified by surfactant hexadecyltrimethylammonium chloride (ZLMS-Cl). The pseudo-second-order kinetic described the adsorption of the dye on all adsorbents. The equilibrium time was reached 40, 60 and 120 min for ZLMS-Br, ZLMS-Cl and ZPMS-Br, respectively. The adsorption equilibrium was analyzed by the equations of the models of linear and nonlinear isotherms of Langmuir, Freundlich, Temkin and Dubinin- Radushkevivh (DR) and the criterion of best fit was evaluated using the error functions.The DR model was adjusted better to the experimental data for the system AL8 / ZLMS-Br, the Freundlich model for AL8 / ZLMS-Cl and Langmuir for AL8 / ZPMS. According to the Langmuir maximum adsorption capacity was 4.67, 1.48 and 1.38 mg g{sup -1} for ZLMS-Br, ZLMS-Cl and ZPMS-Br, in order. In studies employing fixed bed columns, the effects of inlet concentration (20- 30 mg L{sup -1}), flow rate (4.0 -5.3 mL min{sup -1}) and the bed height (5, 5 - 6.5 cm) above the breakthrough curves characteristics in the adsorption system were determined. The Adams-Bohart, Thomas, Yoon-Nelson models were applied to experimental data to predict the breakthrough curves and to determine the parameters that characterize the column. The mathematical models of Thomas and Yoon-Nelson adjusted well to the data of breakthrough curves. The highest bed capacity of 5.3 mg g{sup -1} was obtained using 30 mg L{sup -1} inlet Acid Orange 8 concentration, 5.5 cm bed height and 5.3 mL min{sup -1} flow rate. Acute ecotoxicity tests using Daphnia similis microcrustacean with wastewater (AL8) and after treatment with surfactant modified zeolite were carried out with the purpose of evaluating evidence of possible contamination when launched on the receiving water body. The results of this study showed that samples AL8 dye in aqueous solution does not show any toxic effect, and the treated samples showed toxicity with ZLMS-Br. (author)

  9. Electrocatalytical activity of Pt, SnO{sub 2} and RuO{sub 2} mixed electrodes for the electrooxidation of formic acid and formaldehyde; Atividade eletrocatalitica de eletrodos compostos por Pt, RuO{sub 2} e SnO{sub 2} para a eletrooxidacao de formaldeido e acido formico

    Energy Technology Data Exchange (ETDEWEB)

    Profeti, L.P.R.; Profeti, D.; Olivi, P. [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Filosofia, Ciencias e Letras. Dept. de Quimica

    2005-07-01

    The electrocatalytical activity of binary electrodes of Pt and SnO{sub 2} and ternary electrodes of Pt and SnO{sub 2} and RuO{sub 2} for the electrooxidation of formic acid and formaldehyde was investigated by cyclic voltammetry and chronoamperometry techniques. The electrode materials were prepared by the thermal decomposition of polymeric precursors at 400 deg C. The cyclic voltammetry results showed that the methanol electrooxidation process presents peak potentials for those electrodes approximately 100 mV lower than the values obtained for metallic platinum electrodes. The Pt{sub 0.6}Ru{sub 0.2}Sn{sub 0.2}O{sub y} electrodes presented the highest current density values for potentials lower than the peak potential values. The chronoamperometric experiments also showed that the addition of SnO{sub 2} and RuO{sub 2} contributed for the enhancement of the electrode activity in low potential values. The preparation method was found to be useful to obtain high active materials. (author)

  10. Utility of the dimercapto succinic acid pentavalent ({sup 99m} Tc- DMSA V) in the diagnostic of secondary bone leisure at metastasis of diverse primary tumours. Preliminary study; Utilidad del acido dimercapto succinico pentavalente ({sup 99m} Tc-DMSA V) en el diagnostico de lesiones oseas secundarias a metastasis de diversos tumores primarios. Estudio preliminar

    Energy Technology Data Exchange (ETDEWEB)

    Ortega L, N. [Hospital de Especialidades ' Dr. Antonio Fraga Mouret' Centro Medico Nacional ' La Raza' , IMSS Mexico D.F. (Mexico); Pichardo R, P.A. [Medico Nuclear adscrito al servicio de Medicina Nuclear del Hospital de Oncologia, Centro Medico Nacional Siglo XXI, IMSS, Mexico D.F. (Mexico); Marquez H, A. [Departamento de Medicina Nuclear, IMSS, Mexico D.F. (Mexico)

    2005-07-01

    The more used method in the diagnosis of secondary bone lesions to become cancerous it is by means of having derived of phosphates like it is the {sup 99m}Tc- MDP. The reason of acquiring searching with the radiopharmaceutical {sup 99m}Tc- DMSA V is with the purpose to find other bone lesions that are not visualized with the gammagraphy with diphosphonate and therefore to increase the specificity of the study. (Author)

  11. Optimization of labelling conditions of 15-p-iodophenyl pentadecanoic acid with {sup 123}I and its pharmacokinetics evaluation; Otimizacao das condicoes de marcacao do acido 15-p-iodo fenil pentadecanoico com {sup 123/131} I e sua avaliacao farmacocinetica

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Ione Caselato; Colturato, Maria Tereza; Araujo, Elaine Bortoleti de; Barbosa, Marycel Figols de; Muramoto, Emiko; Pereira, Nilda Petrona Sosa de; Silva, Constanca Pagano Goncalves da; Almeida, Maria Aparecida T.M. de [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    1996-07-01

    The 15-p-iodo-phenyl pentadecanoic acid (IPPA) labelled with {sup 123} I is an important radiopharmaceutical for use in cardiology, due to its favorable physical characteristics and its labelling stability. The labelling procedure studies for the preparation of IPPA {sup 123/131} I was according to the procedures described by Dougan et col. The optimization of the labelling of the labelling condition and product stability were evaluated by radiochemical controls and by biological distribution study using animal models. (author)

  12. Teores de gordura e acidos graxos de clones de cacau nas condições do Vale do Ribeira (SP Fat and fatty acids content of cocoa clones under the conditions of Vale do Ribeira, State of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Maria Luiza Sant'Anna Tucci

    1996-01-01

    Full Text Available O Estado de São Paulo é uma das regiões em que se cultiva o cacaueiro (Theobroma cacao L. mais afastadas do equador, com características climáticas distintas daquelas registradas nas regiões tradicionais de cultivo. A manteiga de cacau apresenta características físico-químicas peculiares, estreitamente relacionadas aos teores dos ácidos graxos que a constituem, os quais são influenciados pelas condições climáticas, especialmente a temperatura do ar. No presente trabalho, caracterizaram-se treze genótipos de cacau, da coleção do Instituto Agronômico, em Pariquera-Açu a 24°43' S, 47°53' W e 25 m de altitude, com base no teor de gordura e na composição em ácidos graxo. Os clones estudados foram os seguintes: SCA 6, SCA 12, IAC 1, ICS 39, ICS 60, ICS 95, UF29, UF 613, UF 667, UF 668, UF 677, P 7 e IMC 67. Houve diferenças significativas entre os clones quanto ao teor de gordura, que variou de 50,7%, para o ICS 95, a 57,6%, para o IAC 1. A composição de ácidos graxos diferiu significativamente entre os clones, exceto para o ácido araquídico. A relação entre ácidos graxos saturados e insaturados variou de 1,37 a 1,74: os clones SCA 12, SCA 6 e P 7 diferiram estatisticamente dos clones ICS 39, 60, 95, IMC 67 e UF 668.The State of São Paulo (Brazil represents the farthest region from the equator in which cocoa (Theobroma cacao L. is cultivated, with different climatic conditions from those observed in the traditional growing areas. Cocoa butter shows peculiar physical and chemical characteristics closely related to fatty acids balance, which are influenced by climatic conditions, mainly air temperature. This paper deals with the characterization of 13 clones of cocoa, growing in the State of São Paulo, based on fat content and fatty acid composition for the climatic conditions of Pariquera-Açu Experimental Station, at 24°43' S, 47°53' W and 25 m of altitude. The clones studied were: SCA 6, SCA 12, IAC 1, ICS 39, ICS 60, ICS 95, OF 29, OF 613, OF 667, OF 668, OF 677, P 7 e IMC 67. Significant differences were found for fat content, which varied from 50.7% for ICS 95 to 57.6% for IAC 1. Fatty acid composition differed significantly among the clones, except for araquidic acid. The saturated and unsaturated fatty acids ratio varied from 1.37 to 1.74, and the clones of SCA 12, SCA 6 and P 7 differed statistically for ICS 39, ICS 60, ICS 95, IMC 67 and OF 668 clones.

  13. Characterization and corrosion behavior of phytic acid coatings, obtained by chemical conversion on magnesium substrates in physiological solution; Caracterizacion y comportamiento frente a la corrosion de recubrimientos de acido fitico, obtenidos por conversion quimica, sobre substratos de magnesio en solucion fisiologica

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez-Alvarado, L. A.; Lomeli, M. A.; Hernandez, L. S.; Miranda, J. M.; Narvaez, L.; Diaz, I.; Garcia-Alonso, M. C.; Escudero, M. L.

    2014-10-01

    In order to improve the corrosion resistance of biodegradable magnesium and AZ31 magnesium alloy implants, a phytic acid coating has been applied on both substrates and their protective effect against corrosion has been assessed. The morphology and the chemical nature of the conversion coating were analyzed by SEM/EDX, XRD and FTIR. The spectra showed that the conversion coating was amorphous, and it was composed of Mg, O, and P on magnesium surface, along with Al, Zn and C on AZ31 alloy. The main coating components were chelate compounds formed by phytic acid and metallic ions. The corrosion resistance of bare and coated samples was evaluated by potentiodynamic polarization technique in Hank's solution at 37 degree centigrade. The results indicate that phytic acid conversion coatings provided a very effective protection to the magnesium substrates studied. (Author)

  14. Seguimiento de la producción del aroma del yogurt durante la fermentación acido láctica mediante nariz electrónica y evaluación sensorial / Monitoring aroma production of yogurt during lactic fermentation by electronic nose and sensory evaluation

    OpenAIRE

    Rúales Guzmán, Bertha Viviana

    2012-01-01

    El yogurt es una de las bebidas fermentadas más consumidas en el mundo por sus propiedades nutricionales, funcionales y sensoriales, por lo tanto la calidad final del producto será de gran importancia para satisfacer las necesidades del consumidor y obtener un posicionamiento en el mercado. El seguimiento de la fermentación es la clave para obtener todas estas propiedades, por tal razón, en la presente investigación se propuso el seguimiento de la producción de aroma como un método alternativ...

  15. Influence of the acid and basic properties of rhenium oxide supported on alumina catalyst on the catalytic performance in olefin metathesis; Influence des proprietes acido-basiques de l`oxyde de rhenium supporte sur les performances catalytiques en metathese des olefines

    Energy Technology Data Exchange (ETDEWEB)

    Nahama, F.

    1996-11-30

    The aim of this work is to study the influence of the acid-basic properties of rhenium oxide supported on alumina catalyst on the catalytic performance in olefin metathesis. The literature data indicate that the environment of the active site does possess acid properties. However, the nature of the acid sites is still matter of debate. Concerning the Re O{sub x} - Al{sub 2}O{sub 3} interactions, we have shown that perrhenate ions are electrostatically absorbed on alumina. The uptake of rhenium is favoured at acidic pH (below 4), and the absorbed rhenium is in equilibrium with rhenium in solution. The results of rhenium extraction by water strongly suggest that the surface compounds of the calcined Re{sub 2}O{sub 7}/Al{sub 2}O{sub 3} materials is aluminium perrhenate. Characterization of surface acidity of the catalyst by infrared spectroscopy reveals that the initiation of the metathesis reaction is governed essentially by Lewis acidity. This strongly supports the role of Lewis acidity, which is exalted by the increase of the rhenium content and the calcination temperature. Finally, we point out by ammonia adsorption-thermodesorption a band at 1320 cm{sup -1} characteristic of the Lewis acidity of aluminium perrhenate. This result is a second indication of the presence of aluminium perrhenate on the Re{sub 2}O{sub 7}/Al{sub 2}O{sub 3} catalyst surface. (author)

  16. Chemical recycling of post-consumer PET: structural characterization of terephthalic acid and the effect of Alkaline Hydrolysis at low temperature; Reciclagem quimica do PET pos-consumo: caracterizacao estrutural do acido tereftalico e efeito da hidrolise alcalina em baixa temperatura

    Energy Technology Data Exchange (ETDEWEB)

    Fonseca, Talitha Granja; Almeida, Yeda Medeiros Bastos de; Vinhas, Gloria Maria, E-mail: gmvinhas@yahoo.com.br [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Engenharia Quimica

    2014-09-15

    Due to the environmental impact caused by PET packaging disposal, this material recycling has been thoroughly discussed and evaluated. In particular, chemical recycling enables achievement of the monomers that are used in PET resin manufacture: ethylene glycol (EG) and terephthalic acid (PTA). Therefore, studies for this process optimization are important from environmental and economic points of view. The present study investigated certain parameters that influence the depolymerization reaction of PET post-consumer via alkaline hydrolysis in order to obtain PTA. Assays were performed at 70 °C by varying the concentration of sodium hydroxide and the reaction time. The best results were obtained at 10.82 mol L{sup -1} NaOH and 9 h reaction time. Consequently, it was possible to prove this process viability, once analyses by infrared and nuclear magnetic resonance confirmed that PTA was obtained in all reactions performed. (author)

  17. Influence of method of preparation of Pt Ru/C electrocatalysts on the catalytic activity for the ethanol oxidation reaction in acidic medium; Influencia do metodo de preparacao de eletrocatalisadores PtRu/C sobre a atividade catalitica frente a reacao de oxidacao de etanol em meio acido

    Energy Technology Data Exchange (ETDEWEB)

    Gomes, Walber dos Santos; Silva, Uriel Lean Valente; Souza, Jose Pio Iudice de, E-mail: jpio@ufpa.br [Universidade Federal do Para, (UFPA), Belem, PA (Brazil). Instituto de Ciencias Exatas e Naturais. Faculdade de Quimica

    2013-09-01

    In this work the influence of variations in the borohydrate reduction method on the properties of Pt Ru/C electrocatalysts was investigated. The electrocatalysts were prepared using 1:1 ; 2:1; 5:1; 50:1 and 250:1 molar ratios of NaBH{sub 4} to metals. The reduction was also performed by dripping or by fast addition of the solution. The results showed that Pt Ru nanoparticles obtained by fast addition had the smallest crystallite sizes. It was also noted that the catalytic activity increased as the borohydrate:metal molar ratio increased. The Pt Ru/C electrocatalysts (50:1) obtained by fast addition presented the best catalytic activity for ethanol electro-oxidation. (author)

  18. The preparation of nucleotides uniformly labelled with carbon-14 by biosynthetic methods. Isolation of adenylic, uridylic, cytidylic,and guanylic acids, from the alkaline hydrolysate of escherichia coli RNA; Preparacion de nucleiotidos uniformemente marcados con 14{sup C}, por via biosintetica. Aislamiento de los acidos adenilico, uridilico, citidilico y guanilico, procedentes de la hidrolisis alcalina de RNA de escherichia Coli.

    Energy Technology Data Exchange (ETDEWEB)

    Garcia Pineda, M. D.; Pacheco Lopez, J.

    1978-07-01

    A method is described for the preparation and analysis of adenylic, uri dilic, cytidi- 11c and guanylic acids, labelled with 14{sup C}. Escherichia coli cells have been labelled by growing them in a medi dia containing glucose-14{sup C} as their only source of carbon. RNA is isolated from the cells, and after hydrolysis of the molecule the resulting nucleotides are separated by gel filtration and exchange chromatography. Chemical and radiochemical purity of the Isolated nucleotides is determined, and also its specific radioactivity. (Author) 30 refs.

  19. Efectividad del Acido Peracético sobre la reducción de la carga de Esporas de Mohos causantes de Pudrición Poscosecha de Frutas y Hortalizas Effectiveness of Peracetic Acid on fungal Spores reduction of Moulds causing rotting in Fruits and Vegetables

    Directory of Open Access Journals (Sweden)

    María V Kyanko

    2010-01-01

    Full Text Available Se evaluó in vitro la efectividad del ácido peracético sobre la reducción de la carga de esporas de mohos micotoxicogénicos causantes de pudrición de frutas y hortalizas, para determinar su potencial aplicación al control poscosecha de esta patología. Se evaluó tres concentraciones de ácido (0.05%, 0.1 % y 0.3% para determinar su capacidad antifúngica frente a Alternaria alternata, Fusarium graminearum, Aspergillus ochraceus, A. níger, A. flavus, Penicillium roqueforti y P. expansum. Se observó una reducción de la carga de esporas aún a la más baja concentración ensayada, efecto que incrementó con el aumento de la concentración del tratamiento. A una concentración del 0.3 % se logró una mucho mayor reducción de la carga de esporas viables de A. alternata, F. graminearum y A. ochraceus, respectivamente. El ácido peracético podría resultar una alternativa de tratamiento no contaminante para el control poscosecha de la pudrición fúngica.The objective of this work was to determine in vitro the effectiveness of peracetic acid on the load reduction of mould spores that are responsible for rotting in fruits and vegetables to evaluate its potential application to post-harvest control. Three concentrations of peracetic acid (0.05%, 0.1% and 0.3% were used to evaluate its anti-fungal capacity against Alternaria Alternata, Fusarium graminearum, Aspergillus ochraceus, A. niger, A. flavus, Penicillium roqueforti and P. expansum. It was observed reduction of the load of spores using the lowest concentration. This effect was increased with a higher concentration of peracetic acid. It was extremely effective at a concentration of 0.3% against A. alternata, F. graminearum and A. ochraceus. It is concluded that the peracetic acid is a nonpolluting alternative treatment for post-harvest rotting control of fruits and vegetables

  20. Assessment of micro, macro, toxic elements (Cd, Hg, Pb) and fatty acids profile in consumed fish commercially available in Cananeia and Cubatao, Sao Paulo State; Avaliacao de micro e macroelementos, elementos toxicos (Cd, Hg e Pb) e acidos graxos, em peixes disponiveis comercialmente para consumo em Cananeia e Cubatao, Estado de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Curcho, Michel Rodrigues da Silva Morales

    2009-07-01

    Aside from their nutritional importance, fish are considered one of the most important bio indicators in aquatic systems for the estimation of pollution levels by toxic metals. This is so, since fish can bio accumulate these elements and they occupy different trophic levels and present different sizes and age. The purpose of the present study was to contribute with important data for the knowledge of nutritional and toxic constituents in muscles of the most consumed fish species from two coastal regions, Cananeia and Cubatao. Cananeia fish species analyzed were: Micropogonias furnieri (Corvina), Macrodon ancylodon (Pescada), Centropomus undecimalis (Robalo peba) and Mugil platanus (Tainha). From Cubatao, Micropogonias furnieri (Corvina), Macrodon ancylodon (Pescada), Menticirrhus americanus (Perna de moca), Sardella braziliensis (Sardinha) and Mugil liza (Tainha). For this study, analytical methodologies were developed and validated, regarding precision and accuracy, by means of certified reference materials. Micronutrients (Ca, Fe, K, Na, Se and Zn) and some trace elements (As, Br, Co, Cr, Rb) in fish muscle were determined by means of neutron activation analysis (NAA). Cold Vapor Atomic Absorption Spectrometry (CV AAS) was used for total Hg determination and Electrothermal Atomic Absorption Spectrometry (ET AAS) for Cd and Pb determinations. Proximate composition determinations according to AOAC methodologies and fatty acids profiles by gas chromatography were done in these muscle fish samples. Regarding fatty acids profile from the {omega}-6 family, Tainha species pool presented the highest values (8.9%) and Pescada species pool the lowest ones (4.4%) for Cananeia species. Fatty acids from the {omega}-3 family, Sardinha pool species presented the highest values (31.8%) for all species analyzed. Regarding micronutrients content great concentration variations between individual of the same species and between different species were observed. Discriminate analysis was used for the values for micronutrient content for all fish species analyzed in order to identify samples of similar chemical composition and respective food habits. Concerning populational risk to toxic elements exposure, in this case As and Hg, it was possible to conclude that Corvina and Pescada from Cananeia and Corvina, Sardinha and Tainha from Cubatao presented the highest values for As exceeding the Brazilian legislation limits for this contaminant. For Hg, all the species analyzed did not exceed the Brazilian legislation limits for predatory and non predatory species. Results from this study also suggest that Corvina species can be used as a bioindicator since this species bioaccumulate metals and remains in the estuary until it reaches adulthood. Thus, Corvina can be considered as a good indicator of the conditions of the coastal region where it inhabits. (author)

  1. Study of conformational and acid-base properties of norbadione A and pulvinic derivatives: Consequences on their complexation properties of alkaline and alkaline earth cations; Etude des proprietes conformationnelles et acido-basiques de la norbadione A et de derives pulviniques: consequences sur leurs proprietes complexantes de cations alcalins et alcalino-terreux

    Energy Technology Data Exchange (ETDEWEB)

    Kuad, P

    2006-01-15

    This work deals with the study of norbadione A, a pigment extracted from mushrooms and known to complex cesium cations. The study of the acid-base properties of norbadione A has allowed to determine the relative acidity of the seven protonable functions of the molecule and to reveal a reversible isomerization of the double exocyclic bond of the pulvinic moieties. The observed change of configuration is induced by a hydrogen bond of the H-O-H type and by electrostatic interactions. Moreover, the microscopic protonation mechanism of the norbadione A has been analyzed, considering three different study media where the acid-base properties of the norbadione A are compared. In the presence of 0.15 mol.l{sup -1} of NaCl, it has been observed a remarkable cooperativity in the protonation of the enol groups. At last, the use of different analytical methods (NMR, potentiometry and calorimetry) has allowed to study the complexing properties of the norbadione A towards cesium and other alkaline and rare earth cations. (O.M.)

  2. Evaluation of mitomycin-C effect on biodistribution of {sup 99m}Tc-dimercaptosuccinic acid in BALB/C mice; Avaliacao do efeito da mitomicina-c na biodistribuicao do radiofarmaco acido dimercaptosuccinico marcado com tecnecio-99m em camundongos BALB/C

    Energy Technology Data Exchange (ETDEWEB)

    Gomes, Maria Luisa; Britto, Deise Mara de M.; Freitas, Rosimeire de S.; Bernardo-Filho, Mario [Instituto Nacional do Cancer, Rio de Janeiro, RJ (Brazil). Centro de Pesquisa Basica; Braga, Ana Cristina de S.; Bezerra, Roberto Jose A.C. [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Dept. de Biofisica e Biometria

    1999-11-01

    The many desirable characteristics of technetium-99m have stimulated the development of labeling techniques for different molecular and cellular structures. It is accepted that a variety of factors such the drug chemotherapy can alter the biodistribution of radiopharmaceuticals. The unknowledge of these factor may result in an unexpected behavior of the radiopharmaceuticals. Since patients on chemotherapeutic treatment can be submitted to a nuclear medicine procedure, we have studied in mice, the effect of mitomycin-C on the {sup 99m} Tc-DMSA used for kidney scintigraphy. Mitomycin-C is an antineoplastic agent obtained from Streptomyces caesptosus. The metabolism of this drug produces different toxic and inactive metabolites. Mitomycin-C 0.15 mg was administered in Balb/c female with an interval of 72 hours. After one hour of the last dose, 0.3 ml of 9{sup 9m}c-DMSA (7.4 MBq) were injected and after 0.5 h the animals were sacrificed. The organs were isolated and counted in a well counter. The percentage of radioactivity (%ATI) were calculated and statistical analysis were performed (Wilcoxon Test). The results have shown that the %ATI: has increased in stomach and uterus, has decreased in kidney and ovary; has not altered in pancreas, spleen, lung, heart, liver, thymus, thyroid, bone and brain. The increase of % ATI could be justified by the metabolic process or the therapeutical effect of mitomycin-C. As an effect of this drug is observed in the uptake of the {sup 99m} Tc-DMSA by the kidney, probably an alteration in the kidney scintigraphy is expected. However, the increase of %ATI of this radiopharmaceutical in other organs by the mitomycin-C could be evaluated carefully. (author) 12 ref., 2 tabs.

  3. Evolution evidence of a basic fluid to an acid based in the analysis of hydrothermal alteration of the geothermic field of the Azufres, Michoacan; Evidencias de evolucion de un fluido basico a acido a partir del analisis de la alteracion hidrotermal del campo geotermico de los Azufres, Michoacan

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez Partida, Eduardo [Universidad Nacional Autonoma de Mexico, Mexico, D.F. (Mexico)

    2000-07-01

    Hydrothermal alteration at the Los Azufres geothermal field is mostly composed of calc-silicate minerals that define a propylitic alteration zone, which shows progressive dehydration with depth and temperature increase. A generalized zoning of the calc-silicate zone can be observed, with zeolites in the upper part and epidote-clinozoisite at the deepest levels. An argillic alteration zone overlies the calc-silicate zone and is the dominant surface manifestation of the hydrothermal alteration. In some parts, there is a mineral assemblage composed of kaolinite-alunite-native sulfur-quartz (advanced argillic zone) formed by the interaction of vapor and shallow groundwater. The proto-fluid at the Los Azufres geothermal system is related to a neutral sodium chlorine brine, which favors deep propyllitic alteration (productive zone). This zone is characterized by secondary permeability due to fracturing. At depth the geothermal field is dominated by a pressurized liquid, yielding to vapor at more shallow zone. The gradual change from a liquid to a vapor phase occurs through boiling at depths between 1,200 and 1,500 m, and is accompanied by changes in the hydrothermal alteration mineralogy. The type of alteration passes from proylitic to argillic by means of an oxidation-acidification process, which includes the participation of a gas, particularly CO{sub 2}. Considering the physicochemical characteristic of the brine and the evolution of the paragenetic sequence, the Los Azufres geothermal field could de considered a model for hydrothermal behavior at ore deposits which develop by boiling and oxidation of low sulfidation fossil hydrothermal fluids. [Spanish] En el campo geotermico de Los Azufres la zona de alteracion hidrotermal esta formada en su mayor parte por calcosilicatos (que definen una zona paragenetica del tipo propilitico), los cuales muestran una deshidratacion progresiva conforme se va profundizando e incrementandose la temperatura. Se puede generalizar un zoneamiento evolutivo para los calcosilicatos predominando las zeolitas hacia la cima y la epidota-clinozoicita en los niveles mas profundos. Le sobreyace una zona de argilitizacion que domina las manifestaciones hidrotermales superficiales. En algunas zonas, se tiene la asociacion caolinita-alunita-azufre nativo-cuarzo (zona argilica avanzada) formada por la interaccion vapor-acuifero someros. En el sistema geotermico de Los Azufres el protofluido esta ligado a una salmuera clorurado-sodica de caracter neutro que propicia una alteracion hidrotermal propilitica profunda (zona productoras) y se caracteriza por tener una permeabilidad secundaria debido al fracturamiento. A profundiad el yacimiento es a liquido dominante presurizado para evolucionar a vapor en su parte mas somera. El cambio gradual de la fase liquida a vapor se da por ebullicion a profundidades del orden de 1,200 a 1,500 m, y esta acompanado por cambios igualmente presentes en la mineralogia de alteracion hidrotermal. Ella pasa de propilitica a argilica por un proceso de oxidacion-acidificacion, en donde la participacion de gases, principalmente de CO{sub 2}, es importante. De acuerdo a las caracteristicas fisicoquimicas de la salmuera y la evolucion en las fases parageneticas, el yacimiento de Los Azufres pudiera ser un modelo de comportamiento hidrotermal en el deposito de las menas que se dan por ebullicion y oxidacion de los fluidos hidrotermales fosiles de baja sulfidacion.

  4. Preparation of manganese salts of carboxylic acids labelled with ''54Mn and comparison with ''54 MnCl{sub 2} in liquid scintillation counting; Preparacion de sales manganosas de acidos alifaticos monocarboxilicos marcados con ''54 MnCl{sub 2} en medidas por centelleo liquido

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez Barquero, L.; Arcos Merino, J. M. los; Grau Malonda, A.

    1992-07-01

    Procedures for liquid scintillation sample preparation of manganese dimethylbutirate, decanoate and palmitate, labelled with 54 Mn are described. their quenching effect, spectral evolution and counting stability along several weeks are analysed in liquid scintillation measurements with Toluene. HISafe II. PCS, instagel. Dioxane-naphtalene and Toluene-alcohol. For comparison, Inorganic 54 MnCl-2 samples are also studied, resulting in acceptable counting stability but showing greater quenching and signs of little spectral degradation against the organic samples. (Author)

  5. Characterization of ZrP{sub 2}O{sub 7} modified with oxalic acid and thermodynamic analysis associated to the uranyl (Vi) sorption; Caracterizacion de ZrP{sub 2}O{sub 7} modificado con acido oxalico y analisis termodinamico asociado a la sorcion de uranilo (VI)

    Energy Technology Data Exchange (ETDEWEB)

    Garcia G, N.; Ordonez R, E. [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico); Solis C, D. A. [Universidad Autonoma del Estado de Mexico, Facultad de Quimica, Paseo Colon y Paseo Tollocan, 50120 Toluca, Estado de Mexico (Mexico); Simoni, E.; Drot, R.; Jeanson, A., E-mail: eduardo.ordonez@inin.gob.mx [Universite Paris-Sud, Institut de Physique Nucleaire, Georges Clemenceau No. 15, Orsay (France)

    2013-10-15

    Several physical and chemical tests were carried out to evaluate the influence of the oxalic acid in the uranium (Vi) sorption on the surface of the zirconium diphosphate. The physical analyses consist of atomic force microscopy (AFM) and total organic coal, and the chemists are the calculation of the reaction heat for the hydrate and sorption processes, the reaction speed constant and the sorption yield in the sodium perchlorate systems and of oxalic acid; these tests allow to corroborate that the oxalic acid influences positively in the uranium (Vi) sorption forming a ternary system of ZrP{sub 2}O{sub 7}/(C{sub 2}O{sub 4})/U(Vi), with a single configuration along the ph interval studied. (Author)

  6. Oxidizing gel formulation for nuclear decontamination: rheological and acidic properties of the organic matrix and its ozonolysis; Formulation d'un gel oxydant a matrice organique applicable a la decontamination nucleaire: proprietes rheologiques, acido-basiques et ozonolyse de la matrice

    Energy Technology Data Exchange (ETDEWEB)

    Rouy, E

    2003-10-15

    An acidic and oxidizing gel was formulated with a purely organic matrix, xanthan gum, at low concentrations (1 to 2 wt %). This polymer gel was investigated in various media (aqueous, acidic and ceric) by means of rheology: shear thinning behaviour, thixotropy, yield stress... Evidences of unexpected rheological properties in highly concentrated media show that xanthan is quite convenient for industrial projection of this type of gel on metallic walls in nuclear plants, notwithstanding its time-limited resistance to oxidation (about a few hours). Complexation mechanisms between ceric species and polar sites of the polymer led us to characterise acidic properties of our xanthan sample by potentiometric titration and {sup 1}H NMR techniques. The matrix was finally treated by ozonolysis to suppress organic residues, as required to handle nuclear wastes. In acidic medium, ozonolysis of the gel was achieved successfully while in acidic and ceric medium this process showed limited efficiency, needing further investigation to be clarified. (author)

  7. Crystallochemical study of amides derived from 6{alpha}, 7{beta}-diidroxivoacapan-17{beta}-oic acid by X-ray diffraction; Estudo cristaloquimico de amidas derivadas do acido 6{alpha}, 7{beta}-di- hidroxivouacapan-17 {beta}-oico por difracao de raios-X

    Energy Technology Data Exchange (ETDEWEB)

    Branco, Marcello Cardoso; Prado Gambardella, Maria Teresa do [Sao Paulo Univ., Sao Carlos, SP (Brazil). Inst. de Quimica. Dept. de Quimica e Fisica Molecular

    1995-12-31

    Abstract. The 6{alpha}, 7{beta}-diidroxivoacapan-17{beta}-oic acid (DVA) is a Furane-diterpene isolated from Peterodon genus. It has anti inflammatory and analgesic properties. The purpose of this work is the characterization of amides derived from DVA, in order to understand the relationship between Chemical Structure and Biological Activity of Vouacapanes. The structures of DVA derivatives will be solved by single-crystal X-ray diffraction. (author) 15 refs., 2 figs.

  8. Crystallochemical study of esters derived from 6{alpha}, 7{beta}-diidroxivouacapan-17{beta}-oic acid by x-ray diffraction; Estudo cristaloquimico de esteres derivados do acido 6{alpha}, 6{beta}-di-hidroxivouacapan-17{beta}-oico por difracao de raios-X

    Energy Technology Data Exchange (ETDEWEB)

    Abrahao Junior, Odonirio [Sao Paulo Univ., Sao Carlos, SP (Brazil). Inst. de Quimica

    1995-12-31

    The 6{alpha}, 7{beta}-diidroxivoacapan-17{beta}-oic acid (DVA) is a Furane-diterpene isolated from Peterodon genus. It has anti-inflammatory and analgesic properties. The purpose of this work is the characterisation by x-ray single crystal diffraction technique of esters derived from DVA, to understand the relationship between chemical structure and biological activity of vouacapanes. (author) 15 refs.

  9. Comparison of the efficacy of carbamazepine, haloperidol and valproic acid in the treatment of children with Sydenham´s chorea: clinical follow-up of 18 patients Comparación de la eficacia de carbamazepina, haloperidol y acido valproico en el tratamiento de niños con corea de Sydenham: seguimiento clínico de 18 pacientes

    Directory of Open Access Journals (Sweden)

    Joaquín Peña

    2002-06-01

    Full Text Available In order to compare and contrast the efficacy of haloperidol, carbamazepine, and valproic acid in the treatment of Sydenham´s chorea a prospective study including 18 cases of this disorder was undertaken. Age of patients ranged from 7 to 15 years. Ten children were female and 8 were male. All but one had generalized, either symmetric or asymmetric chorea. The patients were divided in three equal groups, and were given a standardized dose of each of the drugs built-up over a week. Following therapy, the six children receiving valproic acid showed remarkable improvement, without side effects. Five patients receiving carbamazepine showed improvement without side effects. Only three of the patients that received haloperidol improved. In the 4 cases that did not show clinical improvement after one week of treatment, therapy with valproic acid led to disappearance of the symptoms in a lapse that ranged from 4 to 7 days. Recurrence related to discontinuation of treatment was observed in two patients. In view of the present results we recommend valproic acid as the first choice drug to treat Sydenham chorea.A fin de comparar y contrastar la eficacia de haloperidol, carbamazepina y ácido valproico en el tratamiento de la corea de Sydenham, se realizó un estudio prospectivo que incluyó 18 casos de esta patología. La edad de los pacientes varió de 7 a 15 años. Diez de los niños eran varones y el resto hembras. A excepción de uno de ellos, todos tenían corea generalizada, simétrica ó asimétrica. Los pacientes fueron divididos en tres grupos iguales, a cada uno de los cuales se le administró una dosis estandarizada de los medicamentos mencionados durante una semana. Luego del tratamiento, los seis pacientes que recibieron ácido valproico mostraron mejoría notable sin efectos colaterales. Cinco de los seis pacientes que recibieron carbamazepina exhibieron mejoría sin efectos colaterales. Solo tres de los pacientes que recibieron haloperidol mejoraron. En los cuatro casos que luego de recibir estas dos últimas drogas sin experimentar mejoría clínica luego de una semana, se instaló terapia con ácido valproico, lo que llevó a desaparición de la sintomatología en un lapso de 4 a 7 días. Se observó recaída relacionada con tratamiento discontinuado en dos de los pacientes. A la vista de nuestros resultados, recomendamos el ácido valproico como droga de primera elección en el tratamiento de la corea de Sydenham.

  10. Estabilidad de un análogo de capsaicina : Efecto del pH sobre la reacción de descomposición térrnica de vainillilamida del acido pelargónico (N-[4-hidroxi-3-metoxifenil-metil] nonanamida) en solución hidroalcohólica

    OpenAIRE

    Matias, Rossana L.; Sein, Gustavo O.; González, Miriam; Mandrile, Eloy L.; Cafferata, Lázaro F.R.

    1998-01-01

    La vainillilamida del ácido pelargónico ("capsaicina sintética"), un análogo del compuesto natural N[{4-hidroxi-3-metoxifenil)-metil]-8-metil-(E)-6-nonenamida), es hidrolizada rápidamente en soluciones metanólicas acuosas con valores de pH de 6,O, 8,5 y 10,5 y en un ámbito de temperaturas comprendido entre 50ºC y 100ºC. El análisis cualitativo de los principales productos de la reacción fue realizado utilizando CG-EM. La cinética de la reacción, medida utilizando espectroscopía ultra- violeta...

  11. Radiomimeticity of the system H{sub 2}O{sub 2}/Fe(II) on nucleic acid components. Kinetics study; Radiomimeticidad del sistema H{sub 2}O{sub 2}/Fe(II) sobre components de Acidos Nucleicos. Estudio Cinetico

    Energy Technology Data Exchange (ETDEWEB)

    Cirauqui, R.; Mingot, F.; Davila, C. A.

    1974-07-01

    The kinetic study of the action of a redox system on DNA monomers allowed us to make criticisms on radiomimetic character of this system. Assuming that in both cases, gamma radiolysis of aerated aqueous solutions and action of H{sub 2}O{sub 2} / Fe(II) system in the same conditions, the reactive species is the OH radical, we propose the kinetic expressions that are confirmed by our experimental results. Some of the accepted G-values are corrected in view of our results. Al so these results put in evidence mechanisms of molecular repair after radical attack. (Author) 79 refs.

  12. Polarographic study of the Cu(II)/Cu(I) system in the presence of 1-ascorbic acid and 0,1 M ClK; Estudio polarografico del sistema Cu(II)/Cu(I) en presencia del acido 1-ascorbico y en ClK 0,1 M

    Energy Technology Data Exchange (ETDEWEB)

    Alonso Lopez, J.

    1969-07-01

    While studying the catalytic effect of Cu{sup {down_arrow}}2 ions on the oxidation process of 1-ascorbic acid, it has been observed that, in a 0,1 M solution of K1 at a pH 6 to 7, the above acid gives rise in the presence of Cu{sup {down_arrow}}2 ions to a polarographic wave of half-wave potential of -0,41 V (vs. S.C.E.). (Author) 14 refs.

  13. Determination of the extractive capacity of para-tert butyl calix[8]arene octa-phosphinoylated towards uranyl ions from an aqueous-acidic-salty medium; Determinacion de la capacidad extractiva del p-ter-butilocalix[8]areno octa-fosfinoilado hacia iones uranilo de un medio acuo-acido salino

    Energy Technology Data Exchange (ETDEWEB)

    Serrano V, E. C.

    2011-07-01

    The extraction properties of octa-phosphinoylated para-tert butyl calix[8]arene (prepared in the laboratory) in chloroform towards uranyl ions from an aqueous-acidic-salty medium (HNO{sub 3}-3.5 NaNO{sub 3}) containing uranyl nitrate salt, was investigated. Two spectroscopic techniques UV/Vis and Luminescence were used for this study. The latter permitted analyze the fluorescence from the uranyl ions influenced by the surrounding medium. Both permitted to learn about the power of this calixarene as extractant towards the mentioned ions. Its extraction ability or capability using this calixarene at 5.91 x 10{sup -4} M towards the uranyl ions was 400% as determined by UV/Vis while fluorescence revealed 100% of uranyl ion extraction. A closed analysis of the results obtained by using these techniques revealed that the stoichiometry of the main extracted species was 1calixarene:2 uranyl ions. The loading capacity of the calixarene ligand towards the uranyl ions was also investigated using both techniques. UV/Vis resulted to be inadequate for quantifying exactly the loading capacity of the calixarene whereas luminescence was excellent indeed, using a 5.91 x 10{sup -4} M calixarene concentration, its loading capacity was 0.157 M of free uranyl ions from 0.161 M of uranyl ions present in the aqueous-acidic-salty medium. The extracts from the ability and capacity studies were concentrated to dryness, purified and the dried extracts were analyzed by infrared and neutron activation analysis. By these techniques it was demonstrated that during the extraction of the uranyl ions by the calixarene ligand they form thermodynamically and kinetically stable complexes, since in the solid state, the 1:2, calixarene; uranyl ions stoichiometry was kept with the minimum formula: (UO{sub 2}){sub 2}B{sub 8}bL{sup 8}(NO{sub 3}){sub 4}(H{sub 2}O){sub 4}CHCl{sub 3}(CH{sub 3}OH){sub 3} the methanol molecules come from its purification. It is proposed that B{sub 8}bL{sup 8} calixarene in chloroform medium is a good extractant for the treatment of nuclear wastes or radioactive wastes containing uranium in a rich aqueous-acidic-salty medium. (Author)

  14. Usefulness of the dimercapto succinic pentavalent acid ({sup 99m} Tc- Dmsa-V) in the diagnostic of the bone metastases illness; Utilidad del acido dimercapto succinico pentavalente ({sup 99m} Tc- DMSA-V) en el diagnostico de la enfermedad osea metastasica

    Energy Technology Data Exchange (ETDEWEB)

    Ortega L, N

    2005-07-01

    The objective of this study was to determine the utility of {sup 99m} Tc-(V)- Dmsa whole body planar scan in the diagnostic of skeletal metastases. Nineteen patients were studied, having a recent {sup 99m} Tc-HDP bone scan reporting different pathologies (3 normal scans, 5 equivocal scans and 11 with disseminated bone metastases). 72 hours later, a whole body planar scan was obtained at 3 and 24 hours after the i.v. administration of {sup 99m} Tc-(V)-Dmsa. Counts per pixel were determined in regions of interest drawn over metastases sites and in normal tissue, and were correlated with the bone scan. Statistical analysis was carried out by using the Kruskal-Wallis analysis of variance followed by Mann Whitney U test. Other comparisons were done with Momios, chi square and t Student tests. 273 lesions were studied in the whole body bone scan and 184 lesions in the {sup 99m} Tc-(V)-Dmsa scintigraphy (t Student test n.s.). The tumor to normal tissue ratios were 3.3 (+/- 0.521) and the soft to normal tissue ratios were 1.01 (+/- 0.01), Mann Whitney p< 0.01. The study sensitivity was 92% an overall specificity was 97%. As conclusions we have that {sup 99m} Tc-(V)-Dmsa scintigraphy is an useful choice in the diagnostic of bone metastases when the whole body planar bone scan ({sup 99m} Tc- HDP) is equivocal or abnormal. It also points out other lesions such as in bone and in soft tissue. (Author)

  15. The sealing of second mandibular temporary molar pits and fissure with the laser of Nd: YAG, phosphoric acid and the glass ionomer cement; Selamento de fossulas e fissura de segundo molar deciduo inferior com laser de Nd: YAG, acido fosforico e cimento de ionomero de vidro

    Energy Technology Data Exchange (ETDEWEB)

    Toda, Maria Aparecida

    2003-07-01

    The main of our study was to check the sealing of second mandibular temporary molar pits and fissure, in vitro, with the laser of Nd: YAG, phosphoric acid at 37% and the glass ionomer cement (CIV, Fuji IX GC).The proposal was to check the structural morphologic changes in the laser irradiation upon the enamel surface to watch the pits and fissure sealing with the glass ionomer cement use after the laser irradiation and to verify the efficiency of the 'double conditioning' (phosphoric acid + Nd: YAG). At the same time we watch the evolution of the temperature in the pulp chamber's inside. Our desire was to achieve a therapeutic alternative technic to prevent the dental caries. The Nd: YAG laser parameters were the same: 79 mJ of energy per pulse; frequency of 5 Hz; mean power of 0,4 W; optical fiber on contact of 320 {mu}m diameter; fluency of 99,52 J/ cm{sup 2}, assuming that the only differential was the time of the laser application on the enamel surface. The samples were prepared with this way: Laser Nd: YAG (53 second) + acid + CIV (Fuji IX); Laser Nd: YAG (53 s); Laser Nd: YAG (20 s + 20 s) + acid + CIV; Laser Nd: YAG (20 s + 20 s); Acid + CIV; Control. Through the scanning electron microscopy (MEV) we noticed fusion and resolidification regions due to the laser irradiation and a better adaptation of the glass ionomer cement when we did the 'double conditioning'. Concerning the temperature increase we can conclude that the echeloned period was the best recommended because the temperature was found in a pattern that would not cause any damage to the dental pulp. For future studies we suggest a longer relaxing time between the laser irradiation, a comparative study of this method with other lasers, the use of other sealing materials and the study with the permanent teeth. (author)

  16. Study of the temperature influence during the uranium (Vi) sorption on surface of ZrP{sub 2}O{sub 7} in presence of oxalic and salicylic acid; Estudio de la influencia de la temperatura durante la sorcion de uranio (VI) en la superficie del ZrP{sub 2}O{sub 7} en presencia de acidos oxalico y salicilico

    Energy Technology Data Exchange (ETDEWEB)

    Garcia G, N.

    2013-07-01

    This work studies the effect of temperature on the uranium (Vi) sorption onto zirconium diphosphate in the presence of organic acids (oxalic and salicylic acids). Zirconium diphosphate was synthesized by a chemical condensation reaction and characterized using several analytical techniques, in order to check its purity. This point is very important because the presence of any impurities or secondary phases may interfere with the hydration and sorption process. Prior to the sorption experiments, three batches of zirconium diphosphate were pre-equilibrated with NaClO{sub 4}, oxalic acid or salicylic acid solutions. The hydrated solids were washed and dried and then again characterized in order to study the interactions between organic acids and zirconium diphosphate surface. Uranium sorption onto zirconium diphosphate (pre-equilibrated with NaClO{sub 4}, oxalic acid and salicylic acid solutions) was investigated as a function of ph, organic acid and temperature (20, 40 y 60 grades C). Thermodynamic parameters for the sorption reactions (enthalpy change, entropy change and Gibbs free energy change) were determined from temperature dependence of distribution coefficient by using the Vant Hoff equation. Solids characterization after hydration shows that exist an interaction between organic acids and ZrP{sub 2}O{sub 7}. This fact was confirmed with the microcalorimetry study, the reaction heat for hydration of zirconium diphosphate in NaClO{sub 4} solution was exothermic (-269.59 mJ) and for hydration of zirconium diphosphate in oxalic acid solution was endothermic (53.64 mJ). The experimental results showed important differences in the sorption mechanisms for the reaction of Uranium with ZrP{sub 2}O{sub 7} in the presence and absence of organic acids. For the zirconium diphosphate hydrated with oxalic acid, the sorption percentage was 50% from lowest ph values. For the zirconium diphosphate hydrated with salicylic acid, the initial concentration of uranium was 6 x 10{sup -4} M and a percentage of 10% was observed in the initial reaction and it reached 100% at ph 5.5. A similar behavior was observed for the zirconium diphosphate hydrated with NaClO{sub 4}, but the initial concentration of uranium was 5 x 10{sup -4} M. The temperature had an important effect on the sorption of uranium onto ZrP{sub 2}O{sub 7}. The reaction of uranium with the solid hydrated with NaClO{sub 4} was exothermic from 20 to 60 grades C, and the reaction with the solid hydrated with organic acids was exothermic from 20 to 40 grades C and endothermic from 40 to 60 grades C. In conclusion, the thermodynamic study indicated that the sorption process was activated chemisorption s. (Author)

  17. Innovation in sodium erythorbate production. The use of membrane-reactors

    Energy Technology Data Exchange (ETDEWEB)

    Spigno, G. [Piacenza Univ. Cattolica del S. Cuore, Piacenza (Italy). Ist. di Enologia e Ingegneria Alimentare

    2001-04-01

    Isoascorbic or erythorbic acid is a stereoisomer of ascorbic acid acting as a preservative against oxidation and decoloration, recently approved for food use also in the European Market. Actually erythorbate is produced by means of a complex and long process after bio-fermentation of dextrose. In order to simplify and improve this process a membrane system was conceived able to induce acid 2-ketogluconic diffusion from fermentation broth directly into methanol where it esterficates. Acid 2-ketogluconic methyl ester is the intermediate for erythorbic acid. The principal problem was to perfectly separate water methanol to avoid saponification instead of esterification. [Italian] L'acido eritorbico o isoascorbico, recentemente approvato per uso alimentare anche in Europa, e' uno stereoisomero dell'acido ascorbico e agisce come antiossidante e preservante del colore. Attualmente viene prodotto con un complicato processo dopo fermentazione del destrosio. Per semplificare e migliorare questo processo, e' stato studiato un sistema a membrane in grado di trasferire direttamente l'acido 2-chetogluconico dal brodo di fermentazione al metanolo, dove esterifica. L'estere metilico dell'acido 2-chetogluconico e' l'intermedio dell'acido eritorbico. Il problema maggiore era quello di mantenere separati l'acqua ed il metanolo, per evitare la saponificazione al posto della esterificazione.

  18. Regeneracion adventicia de somaclones de uchuva (physalis peruviana)

    OpenAIRE

    Santana, Gloria E.; Angarita, Antonio

    2011-01-01

    Hipocoti1os de uchuva (Physalis peruvjana L.) fueron utilizados en la inducción de callos y regeneración de plántulas. El medio basal de Murashige y Skoog (MS) fue suplementado con bencil amino purina (BAP) de 0 a 5,0 ppm, acido giberelico (GA3 0 Y 1,0 ppm) en combinacion con acido naftalenoacetico (ANA de 0 a 1.0 ppm) o con acido 2,4 diclorofenoxiacetico (2,4-D  de 0 a 1.0 ppm). La inducción de callos dependió de las interacciones hormonales y condiciones de luz. La regeneración de plántulas...

  19. 78 FR 69076 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-11-18

    ... System (SNPMIS) Records. ] DHA 17 DoD EDHA 17 DoD...... Defense Nutrition Management Information System... & Environmental Health Readiness System--Industrial Hygiene (DOEHRS-IH). DHA 23 EDHA 23 Pharmacy Data...

  20. Lewis acid enhanced switching of the 1,1-dicyanodihydroazulene/vinylheptafulvene photo/thermoswitch

    DEFF Research Database (Denmark)

    Parker, Christian Richard; Tortzen, Christian Gregers; Broman, Søren Lindbæk;

    2011-01-01

    Mild Lewis acids enhance the rate of the thermal conversion of vinylheptafulvene (VHF) to dihydroazulene (DHA). In the absence of light, stronger Lewis acids promote the otherwise photoinduced DHA to VHF conversion....

  1. Nutrition and the Aging Eye

    Science.gov (United States)

    ... more sources of the omega-3 fatty acids DHA and EPA (found in fish, especially salmon) also appeared to ... vitamins lutein and zeaxanthin, and the fatty acids DHA and EPA – in four participant groups. One group is to ...

  2. Omega-3 fats: Good for your heart

    Science.gov (United States)

    ... contain two kinds of omega-3s. These are EPA and DHA. Both have direct benefits for your heart. You ... benefits your heart, but not as directly as EPA and DHA. Still, eating nuts, seeds, and healthy oils as ...

  3. Enhanced production of polyunsaturated fatty acid docosahexaenoic acid by thraustochytrid protists

    Digital Repository Service at National Institute of Oceanography (India)

    Jain, R.; Raghukumar, S.; Chandramohan, D.

    The polyunsaturated fatty acid docosahexaenoic acid (DHA) is an important requirement in the human diet. It is also essential in the nutrition of crustaceans and aquaculture animals. Of the sources available for commercial production of DHA...

  4. EFECTO NEFROPROTECTOR DEL ÁCIDO ASCÓRBICO EN UN MODELO DE DAÑO RENAL INDUCIDO POR GENTAMICINA EN CONEJOS

    Directory of Open Access Journals (Sweden)

    Javier Antonio Padilla Funes

    2013-08-01

    Full Text Available Objetivo. Uno de los efectos secundarios mas notables de la Gentamicina es su nefrotoxicidad. En este estudio se propone el posible efecto nefroprotector del acido ascorbico como barredor de radicales libres de oxigeno. Metodología. Se ejecuto un estudio experimental en 24 conejos machos, raza Nueva Zelanda, cuyo peso fue de 1,5 +/- 0,5 Kg. Los sujetos experimentales fueron distribuidos en tres grupos. El grupo Control se manejo con Gentamicina a dosis de 80 mg/Kg/dia por via IM. El grupo Acido Ascorbico se trato a dosis de 200 mg/kg/dia via IP y Gentamicina 80mg/Kg/dia IM. El grupo Sham se manejo con SSN 0,9% por via IP e IM, durante cinco dias consecutivos, para cada grupo. Se determinaron los siguientes parametros: creatinina serica, N-Acetil s-D glucosaminidasa urinaria (NAG-U, diuresis e histopatologia de las muestras renales. Resultados. La creatinina serica fue significativamente mayor para el grupo Control respecto al grupo Acido Ascorbico (p<0,05. Respecto a la diuresis, se observo una tendencia a la poliuria en el grupo Control. En cuanto a NAG-U, se observo inhibicion completa de la actividad enzimatica en el grupo Acido Ascorbico. El analisis histopatologico demuestra hallazgos de necrosis tubular aguda en el 100% del grupo control, mientras que en el grupo Acido Ascorbico se obtuvo en un 50% de los sujetos unicamente detritos intraluminales. Conclusiones. La administracion concomitante de Acido Ascorbico con Gentamicina, disminuye significativamente el dano tubular renal, evidenciado en los valores de creatinina serica, NAG, diuresis e histopatologia renal.

  5. The influence of long chain polyunsaturate supplementation on docosahexaenoic acid and arachidonic acid in baboon neonate central nervous system

    Directory of Open Access Journals (Sweden)

    Sarkadi-Nagy Eszter A

    2005-06-01

    Full Text Available Abstract Background Docosahexaenoic acid (DHA and arachidonic acid (ARA are major components of the cerebral cortex and visual system, where they play a critical role in neural development. We quantitatively mapped fatty acids in 26 regions of the four-week-old breastfed baboon CNS, and studied the influence of dietary DHA and ARA supplementation and prematurity on CNS DHA and ARA concentrations. Methods Baboons were randomized into a breastfed (B and four formula-fed groups: term, no DHA/ARA (T-; term, DHA/ARA supplemented (T+; preterm, no DHA/ARA (P-; preterm and DHA/ARA supplemented (P+. At four weeks adjusted age, brains were dissected and total fatty acids analyzed by gas chromatography and mass spectrometry. Results DHA and ARA are rich in many more structures than previously reported. They are most concentrated in structures local to the brain stem and diencephalon, particularly the basal ganglia, limbic regions, thalamus and midbrain, and comparatively lower in white matter. Dietary supplementation increased DHA in all structures but had little influence on ARA concentrations. Supplementation restored DHA concentrations to levels of breastfed neonates in all regions except the cerebral cortex and cerebellum. Prematurity per se did not exert a strong influence on DHA or ARA concentrations. Conclusion 1 DHA and ARA are found in high concentration throughout the primate CNS, particularly in gray matter such as basal ganglia; 2 DHA concentrations drop across most CNS structures in neonates consuming formulas with no DHA, but ARA levels are relatively immune to ARA in the diet; 3 supplementation of infant formula is effective at restoring DHA concentration in structures other than the cerebral cortex. These results will be useful as a guide to future investigations of CNS function in the absence of dietary DHA and ARA.

  6. Bioavailability of fatty acids from krill oil, krill meal and fish oil in healthy subjects--a randomized, single-dose, cross-over trial

    OpenAIRE

    Köhler, Anton; Sarkkinen, Essi; Tapola, Niina; Niskanen, Tarja; Bruheim, Inge

    2015-01-01

    Background Krill contains two marine omega-3 polyunsaturated fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), mainly bound in phospholipids. Typical products from krill are krill oil and krill meal. Fish oils contain EPA and DHA predominantly bound in triglycerides. The difference in the chemical binding of EPA and DHA has been suggested to affect their bioavailability, but little is known on bioavailability of EPA and DHA in krill meal. This study was undertaken to co...

  7. Docosahexaenoic acid-enriched fish oil attenuates kidney disease and prolongs median and maximal life span of autoimmune lupus-prone mice

    OpenAIRE

    Ganesh V. Halade; Rahman, Md Mizanur; Bhattacharya, Arunabh; Barnes, Jeffery; Chandrasekar, Bysani; Fernandes, Gabriel

    2010-01-01

    The therapeutic efficacy of individual components of fish oils (FO) in various human inflammatory diseases still remains unresolved, possibly due to low levels of n-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) or lower ratio of DHA to EPA. Since FO enriched with DHA (FO-DHA) or EPA (FO-EPA) has become available recently, we investigated their efficacy on survival and inflammatory kidney disease in a well-established animal model of human Systemic Lupus Erythematosu...

  8. Inhibitory effects of omega-3 fatty acids on injury-induced epidermal growth factor receptor transactivation contribute to delayed wound healing

    OpenAIRE

    Turk, Harmony F.; Monk, Jennifer M.; Fan, Yang-Yi; Callaway, Evelyn S.; Weeks, Brad; Chapkin, Robert S.

    2013-01-01

    Epidermal growth factor receptor (EGFR)-mediated signaling is required for optimal intestinal wound healing. Since n-3 polyunsaturated fatty acids (PUFA), specifically docosahexaenoic acid (DHA), alter EGFR signaling and suppress downstream activation of key signaling pathways, we hypothesized that DHA would be detrimental to the process of intestinal wound healing. Using a mouse immortalized colonocyte model, DHA uniquely reduced EGFR ligand-induced receptor activation, whereas DHA and its m...

  9. Acute appearance of fatty acids in human plasma – a comparative study between polar-lipid rich oil from the microalgae Nannochloropsis oculata and krill oil in healthy young males

    OpenAIRE

    Kagan, Michael L; West, Annette L.; Zante, Christa; Calder, Philip C.

    2013-01-01

    Background The long-chain n-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have human health benefits. Alternatives to fish as sources of EPA and DHA are needed. Oil from the micro-algae Nannochloropsis oculata contains a significant amount of EPA conjugated to phospholipids and glycolipids and no DHA. Krill oil contains EPA and DHA conjugated to phospholipids. We compare the appearance of fatty acids in blood plasma of healthy humans after consuming a high fat meal ...

  10. Eicosapentaenoic and docosahexaenoic acid ethyl esters differentially enhance B-cell activity in murine obesity[S

    OpenAIRE

    Teague, Heather; Harris, Mitchel; Fenton, Jenifer; Lallemand, Perrine; Shewchuk, Brian M.; Shaikh, Saame Raza

    2014-01-01

    EPA and DHA are not biologically equivalent; however, their individual activity on B cells is unknown. We previously reported fish oil enhanced murine B-cell activity in obesity. To distinguish between the effects of EPA and DHA, we studied the ethyl esters of EPA and DHA on murine B-cell function as a function of time. We first demonstrate that EPA and DHA maintained the obese phenotype, with no improvements in fat mass, adipose inflammatory cytokines, fasting insulin, or glucose clearance. ...

  11. Docosahexaenoic Acid Ameliorates Fructose-Induced Hepatic Steatosis Involving ER Stress Response in Primary Mouse Hepatocytes

    OpenAIRE

    Jinying Zheng; Chuan Peng; Yanbiao Ai; Heng Wang; Xiaoqiu Xiao; Jibin Li

    2016-01-01

    The increase in fructose consumption is considered to be a risk factor for developing nonalcoholic fatty liver disease (NAFLD). We investigated the effects of docosahexaenoic acid (DHA) on hepatic lipid metabolism in fructose-treated primary mouse hepatocytes, and the changes of Endoplasmic reticulum (ER) stress pathways in response to DHA treatment. The hepatocytes were treated with fructose, DHA, fructose plus DHA, tunicamycin (TM) or fructose plus 4-phenylbutyric acid (PBA) for 24 h. Intra...

  12. The composition of polyunsaturated fatty acids in erythrocytes of lactating mothers and their infants

    DEFF Research Database (Denmark)

    Jørgensen, M.H.; Nielsen, P.K.; Michaelsen, K.F.;

    2006-01-01

    Long-chain polyunsaturated fatty acids (LCPUFA) in breastmilk, specifically docosahexaenoic acid (DHA), are important for infant brain development. Accretion of DHA in the infant brain is dependent on DHA-status, intake and metabolism. The aim of this study was to describe changes in maternal and...

  13. The effects of docosahexaenoic acid on glial derived neurotrophic factor and neurturin in bilateral rat model of Parkinson's disease.

    Directory of Open Access Journals (Sweden)

    Gokhan Akkoyunlu

    2010-11-01

    Full Text Available Parkinson's disease (PD is the second most common neurodegenerative disorder marked by cell death in the Substantia nigra (SN. Docosahexaenoic acid (DHA is the major polyunsaturated fatty acid (PUFA in the phospholipid fraction of the brain and is required for normal cellular function. Glial cell line derived neurotrophic factor (GDNF and neurturin (NTN are very potent trophic factors for PD. The aim of the study was to evaluate the neuroprotective effects of GDNF and NTN by investigating their immunostaining levels after administration of DHA in a model of PD. For this reason we hypothesized that DHA administration of PD might alter GDNF, NTN expression in SN. MPTP neurotoxin that induces dopaminergic neurodegeneration was used to create the experimental Parkinsonism model. Rats were divided into; control, DHA-treated (DHA, MPTP-induced (MPTP, MPTP-induced+DHA-treated (MPTP+DHA groups. Dopaminergic neuron numbers were clearly decreased in MPTP, but showed an increase in MPTP+DHA group. As a result of this, DHA administration protected dopaminergic neurons as shown by tyrosine hydroxylase immunohistochemistry. In the MPTP+DHA group, GDNF, NTN immunoreactions in dopaminergic neurons were higher than that of the MPTP group. In conclusion, the characterization of GDNF and NTN will certainly help elucidate the mechanism of DHA action, and lead to better strategies for the use of DHA to treat neurodegenerative diseases.

  14. On the bromination of the dihydroazulene/vinylheptafulvene photo-/thermoswitch

    DEFF Research Database (Denmark)

    Mazzanti, Virginia; Cacciarini, Martina; Broman, Søren Lindbæk;

    2012-01-01

    Background: The dihydroazulene (DHA)/vinylheptafulvene (VHF) system (with two cyano groups at C1) functions as a photo-/thermoswitch. Direct ionic bromination of DHA has previously furnished a regioselective route to a 7,8-dibromide, which by elimination was converted to a 7-bromo-substituted DHA...

  15. Phosphoserine Lyase Deoxyribozymes: DNA-Catalyzed Formation of Dehydroalanine Residues in Peptides.

    Science.gov (United States)

    Chandrasekar, Jagadeeswaran; Wylder, Adam C; Silverman, Scott K

    2015-08-01

    Dehydroalanine (Dha) is a nonproteinogenic electrophilic amino acid that is a synthetic intermediate or product in the biosynthesis of several bioactive cyclic peptides such as lantibiotics, thiopeptides, and microcystins. Dha also enables labeling of proteins and synthesis of post-translationally modified proteins and their analogues. However, current chemical approaches to introducing Dha into peptides have substantial limitations. Using in vitro selection, here we show that DNA can catalyze Zn(2+) or Zn(2+)/Mn(2+)-dependent formation of Dha from phosphoserine (pSer), i.e., exhibit pSer lyase activity, a fundamentally new DNA-catalyzed reaction. Two new pSer lyase deoxyribozymes, named Dha-forming deoxyribozymes 1 and 2 (DhaDz1 and DhaDz2), each function with multiple turnover on the model hexapeptide substrate that was used during selection. Using DhaDz1, we generated Dha from pSer within an unrelated linear 13-mer peptide. Subsequent base-promoted intramolecular cyclization of homocysteine into Dha formed a stable cystathionine (thioether) analogue of the complement inhibitor compstatin. These findings establish the fundamental catalytic ability of DNA to eliminate phosphate from pSer to form Dha and suggest that with further development, pSer lyase deoxyribozymes will have broad practical utility for site-specific enzymatic synthesis of Dha from pSer in peptide substrates.

  16. Does genetic variation in the Δ6-desaturase promoter modify the association between α-linolenic acid and the prevalence of metabolic syndrome?123

    OpenAIRE

    Truong, Hong; DiBello, Julia R; Ruiz-Narvaez, Edward; Kraft, Peter; Campos, Hannia; Baylin, Ana

    2009-01-01

    Background: Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are associated with protection against components of the metabolic syndrome, but the role of α-linolenic acid (ALA), the metabolic precursor of EPA and DHA, has not been studied. The Δ6-desaturase enzyme converts ALA into EPA and DHA, and genetic variation in the Δ6-desaturase gene (FADS2) may affect this conversion.

  17. Polymorphisms in the fatty acid desaturase genes and diet are important determinants of infant docosahexaenoic acid status

    DEFF Research Database (Denmark)

    Lauritzen, L.; Harsløf, L.; Larsen, L.H.;

    2013-01-01

    Tissue docosahexaenoic acid (DHA) accretion in early infancy is supported by DHA in breast-milk and may thus decrease once complementary feeding takes over. Endogenous synthesis of DHA from alphalinolenic acid is low and polymorphisms in the genes that encodes the fatty acid desaturases (FADS) has...

  18. Does aging change docosahexaenoic acid homeostasis? Implications for the challenge to cognitive health in the elderly

    Directory of Open Access Journals (Sweden)

    Castellano Christian-Alexandre

    2011-07-01

    Full Text Available Epidemiological studies fairly convincingly suggest that higher intake of fish and omega-3 fatty acids present in fish is associated with reduced risk for age-related cognitive decline (ARCD. Normally, docosahexaenoic acid (DHA in plasma is positively associated with DHA intake. However, despite being associated with lower fish and DHA intake, unexpectedly, ARCD is not consistently associated with lower plasma DHA. Furthermore, DHA is often slightly but significantly higher in plasma and erythrocytes in the elderly without ARCD compared to young adults. Higher plasma DHA in the elderly may be a sign that their fish or DHA intake is higher but we show here that various aspects of DHA homeostasis also change with age. Our supplementation and tracer studies show that DHA metabolism, e.g. transit through the plasma and apparent retroconversion but not beta-oxidation, is different in healthy elderly compared to healthy young adults. Apolipoprotein E4 increases the risk of ARCD, possibly in part because it changes DHA homeostasis. Therefore, independent of differences in fish intake, changing DHA homeostasis may contribute to making the elderly more susceptible to cognitive decline despite them having similar or sometimes higher plasma DHA than in younger adults.

  19. 魚のエイコサペンタエン酸及びドコサヘキサエン酸含量に及ぼす調理方法の影響(第1報)

    OpenAIRE

    玉利, 正人; 池田, まどか; 久冨, 貴子; 青山, 佐和子

    1994-01-01

    We wish to report on the effect of cooking on the properties and amounts of eicosapentaenoic acid (EPA) and dosahexaenoic acid (DHA) in fish, pacific saury. Change of EPA and DHA contents by cooking was investigated by gas chromatography. The contents of EPA and DHA of pacific saury were reduced by cooking. The most profound decreases in EPA and DHA contents occurred when pacific saury was fried. It has been demonstrated that standard EPA and DHA were decomposed about 50% by heating for 10 mi...

  20. 魚のエイコサペンタエン酸及びドコサヘキサエン酸含量に及ぼす調理方法の影響(第2報)

    OpenAIRE

    玉利, 正人; 池田, まどか; 久冨, 貴子; 青山, 佐和子

    1994-01-01

    We wish to report on the effect of cooking on the properties and amounts of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in fish, Pasific Saury. Change of EPA and DHA contents by cooking was investigated by gas chromatography. The contents of EPA and DHA of pacific saury were reduced by cooking. The most profound decreases in EPA and DHA contents occurred when pacific saury was broiled and boiled. The EPA and DHA were decreased to about 36% and 37% of the initial values by cooki...

  1. Plasma non-esterified docosahexaenoic acid is the major pool supplying the brain.

    Science.gov (United States)

    Chen, Chuck T; Kitson, Alex P; Hopperton, Kathryn E; Domenichiello, Anthony F; Trépanier, Marc-Olivier; Lin, Lauren E; Ermini, Leonardo; Post, Martin; Thies, Frank; Bazinet, Richard P

    2015-10-29

    Despite being critical for normal brain function, the pools that supply docosahexaenoic acid (DHA) to the brain are not agreed upon. Using multiple kinetic models in free-living adult rats, we first demonstrate that DHA uptake from the plasma non-esterified fatty acid (NEFA) pool predicts brain uptake of DHA upon oral administration, which enters the plasma NEFA pool as well as multiple plasma esterified pools. The rate of DHA loss by the brain is similar to the uptake from the plasma NEFA pool. Furthermore, upon acute iv administration, although more radiolabeled lysophosphatidylcholine (LPC)-DHA enters the brain than NEFA-DHA, this is due to the longer plasma half-life and exposure to the brain. Direct comparison of the uptake rate of LPC-DHA and NEFA-DHA demonstrates that uptake of NEFA-DHA into the brain is 10-fold greater than LPC-DHA. In conclusion, plasma NEFA-DHA is the major plasma pool supplying the brain.

  2. Enhanced Amelioration of High-Fat Diet-Induced Fatty Liver by Docosahexaenoic Acid and Lysine Supplementations

    Directory of Open Access Journals (Sweden)

    Hsin-Yu Lin

    2014-01-01

    Full Text Available Fatty liver disease is the most common pathological condition in the liver. Here, we generated high-fat diet-(HFD- induced nonalcoholic fatty liver disease (NAFLD in mice and tested the effects of docosahexaenoic acid (DHA and lysine during a four-week regular chow (RCfeeding. Our results showed that 1% lysine and the combination of 1% lysine + 1% DHA reduced body weight. Moreover, serum triglyceride levels were reduced by 1% DHA and 1% lysine, whereas serum alanine transaminase activity was reduced by 1% DHA and 1% DHA + 0.5% lysine. Switching to RC reduced hepatic lipid droplet accumulation, which was further reduced by the addition of DHA or lysine. Furthermore, the mRNA expressions of hepatic proinflammatory cytokines were suppressed by DHA and combinations of DHA + lysine, whereas the mRNA for the lipogenic gene, acetyl-CoA carboxylase 1 (ACC1, was suppressed by DHA. In the gonadal adipose tissues, combinations of DHA and lysine inhibited mRNA expression of lipid metabolism-associated genes, including ACC1, fatty acid synthase, lipoprotein lipase, and perilipin. In conclusion, the present study demonstrated that, in conjunction with RC-induced benefits, supplementation with DHA or lysine further ameliorated the high-fat diet-induced NAFLD and provided an alternative strategy to treat, and potentially prevent, NAFLD.

  3. Dihydroxyacetone production in an engineered Escherichia coli through expression of Corynebacterium glutamicum dihydroxyacetone phosphate dephosphorylase.

    Science.gov (United States)

    Jain, Vishist Kumar; Tear, Crystal Jing Ying; Lim, Chan Yuen

    2016-05-01

    Dihydroxyacetone (DHA) has several industrial applications such as a tanning agent in tanning lotions in the cosmetic industry; its production via microbial fermentation would present a more sustainable option for the future. Here we genetically engineered Escherichia coli (E. coli) for DHA production from glucose. Deletion of E. coli triose phosphate isomerase (tpiA) gene was carried out to accumulate dihydroxyacetone phosphate (DHAP), for use as the main intermediate or precursor for DHA production. The accumulated DHAP was then converted to DHA through the heterologous expression of Corynebacterium glutamicum DHAP dephosphorylase (cghdpA) gene. To conserve DHAP exclusively for DHA production we removed methylglyoxal synthase (mgsA) gene in the ΔtpiA strain. This drastically improved DHA production from 0.83g/l (0.06g DHA/g glucose) in the ΔtpiA strain bearing cghdpA to 5.84g/l (0.41g DHA/g glucose) in the ΔtpiAΔmgsA double mutant containing the same gene. To limit the conversion of intracellular DHA to glycerol, glycerol dehydrogenase (gldA) gene was further knocked out resulting in a ΔtpiAΔmgsAΔgldA triple mutant. This triple mutant expressing the cghdpA gene produced 6.60g/l of DHA at 87% of the maximum theoretical yield. In summary, we demonstrated an efficient system for DHA production in genetically engineered E. coli strain. PMID:26992791

  4. Evaluation of docosahexaenoic acid in a dog model of hypertension induced left ventricular hypertrophy.

    Science.gov (United States)

    Stanley, William C; Cox, James W; Asemu, Girma; O'Connell, Kelly A; Dabkowski, Erinne R; Xu, Wenhong; Ribeiro, Rogerio F; Shekar, Kadambari C; Hoag, Stephen W; Rastogi, Sharad; Sabbah, Hani N; Daneault, Caroline; des Rosiers, Christine

    2013-12-01

    Marine n-3 polyunsaturated fatty acids alter cardiac phospholipids and prevent cardiac pathology in rodents subjected to pressure overload. This approach has not been evaluated in humans or large animals with hypertension-induced pathological hypertrophy. We evaluated docosahexaenoic acid (DHA) in old female dogs with hypertension caused by 16 weeks of aldosterone infusion. Aldosterone-induced hypertension resulted in concentric left ventricular (LV) hypertrophy and impaired diastolic function in placebo-treated dogs. DHA supplementation increased DHA and depleted arachidonic acid in cardiac phospholipids, but did not improve LV parameters compared to placebo. Surprisingly, DHA significantly increased serum aldosterone concentration and blood pressure compared to placebo. Cardiac mitochondrial yield was decreased in placebo-treated hypertensive dogs compared to normal animals, which was prevented by DHA. Extensive analysis of mitochondrial function found no differences between DHA and placebo groups. In conclusion, DHA did not favorably impact mitochondrial or LV function in aldosterone hypertensive dogs.

  5. A method for the determination of ascorbic acid using the iron(II)-pyridine-dimethylglyoxime complex

    Energy Technology Data Exchange (ETDEWEB)

    Arya, S. P.; Mahajan, M. [Haryana, Kurukshetra Univ. (India). Dept. of Chemistry

    1998-05-01

    A simple and rapid spectrophotometric method for the determination of ascorbic acid is proposed. Ascorbic acid reduces iron (III) to iron (II) which forms a red colored complex with dimethylglyoxime in the presence of pyridine. The absorbance of the resulting solution is measured at 514 nm and a linear relationship between absorbance and concentration of ascorbic acid is observed up to 14 {mu}g ml{sup -1}. Studies on the interference of substances usually associated with ascorbic acid have been carried out and the applicability of the method has been tested by analysing pharmaceutical preparations of vitamin C. [Italiano] Si propone un rapido e semplice metodo spettrofotometrico per la determinazione dell`acido ascorbico. L`acido ascorbico riduce il ferro(III) a ferro(II) che forma con la dimetilgliossima, in presenza di piridina, un complesso colorato in rosso. L`assorbanza della soluzione risultante e` misurata a 514 nm e si ottiene una relazione lineare tra assorbanza e concentrazione dell`acido ascorbico fino a 14 {mu}g ml{sup -1}. Si sono condotti studi sugli interferenti usualmente associati all`acido ascorbico ed e` stata valutata l`applicabilita` del metodo all`analisi di preparati farmaceutici di vitamina C.

  6. Inhibition of fructan-fermenting equine fecal bacteria and Streptococcus bovis by hops (Humulus lupulus L.) ß-acid

    Science.gov (United States)

    Aims: The goals were to determine if the '-acid from hops (Humulus lupulus L.) could be used to control fructan fermentation by equine hindgut microorganisms, and to verify the antimicrobial mode of action on the Streptococcus bovis, which has been implicated in fructan fermentation, hindgut acidos...

  7. Disease: H00243 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available iology of renal tubular acidosis. Curr Genomics 10:51-9 (2009) PMID:11045400 (gene) Rodriguez-Soriano J New ...rol 14:1121-36 (2000) PMID:12138150 (gene, env_factor, marker, drug) Rodriguez Soriano J Renal tubular acido

  8. Revista de revistas

    OpenAIRE

    Revista, Facultad de Medicina

    2011-01-01

    The Lancet. Vol. I, 1947: 775-811. Londres, junio 7 de 1947 / Aspectos sociales de la epilepsia. Tylor Fox; Lingfield Epilecptic Colony, Surrey. Pp: 775-778 / Virus y enfermedad (Editorial). P: 793-794 / Acido fólico (Editorial). Pp. 795-797

  9. Dietary Crude Lecithin Increases Systemic Availability of Dietary Docosahexaenoic Acid with Combined Intake in Rats.

    Science.gov (United States)

    van Wijk, Nick; Balvers, Martin; Cansev, Mehmet; Maher, Timothy J; Sijben, John W C; Broersen, Laus M

    2016-07-01

    Crude lecithin, a mixture of mainly phospholipids, potentially helps to increase the systemic availability of dietary omega-3 polyunsaturated fatty acids (n-3 PUFA), such as docosahexaenoic acid (DHA). Nevertheless, no clear data exist on the effects of prolonged combined dietary supplementation of DHA and lecithin on RBC and plasma PUFA levels. In the current experiments, levels of DHA and choline, two dietary ingredients that enhance neuronal membrane formation and function, were determined in plasma and red blood cells (RBC) from rats after dietary supplementation of DHA-containing oils with and without concomitant dietary supplementation of crude lecithin for 2-3 weeks. The aim was to provide experimental evidence for the hypothesized additive effects of dietary lecithin (not containing any DHA) on top of dietary DHA on PUFA levels in plasma and RBC. Dietary supplementation of DHA-containing oils, either as vegetable algae oil or as fish oil, increased DHA, eicosapentaenoic acid (EPA), and total n-3 PUFA, and decreased total omega-6 PUFA levels in plasma and RBC, while dietary lecithin supplementation alone did not affect these levels. However, combined dietary supplementation of DHA and lecithin increased the changes induced by DHA supplementation alone. Animals receiving a lecithin-containing diet also had a higher plasma free choline concentration as compared to controls. In conclusion, dietary DHA-containing oils and crude lecithin have synergistic effects on increasing plasma and RBC n-3 PUFA levels, including DHA and EPA. By increasing the systemic availability of dietary DHA, dietary lecithin may increase the efficacy of DHA supplementation when their intake is combined. PMID:27038174

  10. Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells

    Institute of Scientific and Technical Information of China (English)

    Piet Habbel; Karsten H Weylandt; Katja Lichopoj; Johannes Nowak; Martin Purschke; Jing-Dong Wang; Cheng-Wei He; Daniel C Baumgart; Jing X Kang

    2009-01-01

    AIM: To investigate the impact of arachidonic acid (AA) and docosahexaenoic acid (DHA) and their combination on colon cancer cell growth.METHODS: The LS-174T colon cancer cell line was used to study the role of the prostaglandin precursor AA and the omega-3 polyunsaturated fatty acid DHA on cell growth. Cell viability was assessed in XTT assays. For analysis of cell cycle and cell death, flow cytometry and DAPI staining were applied. Expression of cyclooxygenase-2 (COX-2), p21 and bcl-2 in cells incubated with AA or DHA was examined by real-time RT-PCR. Prostaglandin E2 (PGE2) generation in the presence of AA and DHA was measured using a PGE2ELISA.RESULTS: AA increased cell growth, whereas DHA reduced viability of LS 174T cells in a time- and dosedependent manner. Furthermore, DHA down- regulated mRNA of bcl-2 and up-regulated p21. Interestingly,DHA was able to suppress AA-induced cell proliferation and significantly lowered AA-derived PGE2 formation.DHA also down-regulated COX-2 expression. In addition to the effect on PGE2 formation, DHA directly reduced PGE2-induced cell proliferation in a dosedependent manner.CONCLUSION: These results suggest that DHA can inhibit the pro-proliferative effect of abundant AA or PGE2.

  11. The Effect of Omega-3 Docosahexaenoic Acid Supplementation on Gestational Length: Randomized Trial of Supplementation Compared to Nutrition Education for Increasing n-3 Intake from Foods

    Directory of Open Access Journals (Sweden)

    Mary A. Harris

    2015-01-01

    Full Text Available Objective. DHA supplementation was compared to nutrition education to increase DHA consumption from fish and DHA fortified foods. Design. This two-part intervention included a randomized double-blind placebo controlled DHA supplementation arm and a nutrition education arm designed to increase intake of DHA from dietary sources by 300 mg per day. Setting. Denver Health Hospitals and Clinics, Denver, Colorado, USA. Population. 871 pregnant women aged 18–40 were recruited between16 and 20 weeks of gestation of whom 564 completed the study and complete delivery data was available in 505 women and infants. Methods. Subjects received either 300 or 600 mg DHA or olive oil placebo or nutrition education. Main Outcome Variable. Gestational length. Results. Gestational length was significantly increased by 4.0–4.5 days in women supplemented with 600 mg DHA per day or provided with nutrition education. Each 1% increase in RBC DHA at delivery was associated with a 1.6-day increase in gestational length. No significant effects on birth weight, birth length, or head circumference were demonstrated. The rate of early preterm birth (1.7% in those supplemented with DHA (combined 300 and 600 mg/day was significantly lower than in controls. Conclusion. Nutrition education or supplementation with DHA can be effective in increasing gestational length.

  12. Inhibition of lymphangiogenesis, nodal and lung metastasis by dihydroartemisinin in mice bearing Lewis lung carcinoma

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Objective: To investigate the activity of anti-malarial dihydroartemisinin (DHA) on tumor growth, lymphangiogenesis, nodal and lung metastasis and survival in mice bearing Lewis lung carcimoma (LLC). Methods: The models of C57BL/6 mice transplantation tumors were established via subcutaneous injection of LLC cells and divided into 4 groups: control group, DHA group, DHA + ferrous sulfate (FS) group and FS group, with 25 mice in each group. Tumor volumes and weights, nodal and lung metastasis, and survival were monitored. Tumor lymphatic microvessel density (LMVD) was determined by lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) immnohistochemistry. After LLC cells were treated with DHA or DHA + FS, protein and mRNA levels of vascular endothelial growth factor (VEGF) -C were evaluated by Western blotting and real time quantitative RT-PCR, respectively. Results: Oral administration of DHA or DHA+FS inhibited lymph node and lung metastasis, and prolonged survival. However, no significant tumor growth retardation effect was observed when mice were treated with DHA alone. The inhibited tumor metastasis was related to the decreased LMVD in the peritumoral regions, but not in the in-tratumoral regions. DHA significantly down-regulated the expression of VEGF-C protein and mRNA in LLC cells. Conclusion; DHA effectively inhibits LLC transplantation tumor lymphangiogenesis, nodal and lung metastasis, and may be a promising chemotherapeutic agent for controlling lung cancer metastasis by decreasing VEGF-C expression.

  13. Dihydroartemisinin attenuates lipopolysaccharide-induced osteoclastogenesis and bone loss via the mitochondria-dependent apoptosis pathway.

    Science.gov (United States)

    Dou, C; Ding, N; Xing, J; Zhao, C; Kang, F; Hou, T; Quan, H; Chen, Y; Dai, Q; Luo, F; Xu, J; Dong, S

    2016-01-01

    Dihydroartemisinin (DHA) is a widely used antimalarial drug isolated from the plant Artemisia annua. Recent studies suggested that DHA has antitumor effects utilizing its reactive oxygen species (ROS) yielding mechanism. Here, we reported that DHA is inhibitory on lipopolysaccharide (LPS)-induced osteoclast (OC) differentiation, fusion and bone-resorption activity in vitro. Intracellular ROS detection revealed that DHA could remarkably increase ROS accumulation during LPS-induced osteoclastogenesis. Moreover, cell apoptosis was also increased by DHA treatment. We found that DHA-activated caspase-3 increased Bax/Bcl-2 ratio during LPS-induced osteoclastogenesis. Meanwhile, the translocation of apoptotic inducing factor (AIF) and the release of cytochrome c from the mitochondria into the cytosol were observed, indicating that ROS-mediated mitochondrial dysfunction is crucial in DHA-induced apoptosis during LPS-induced osteoclastogenesis. In vivo study showed that DHA treatment decreased OC number, prevents bone loss, rescues bone microarchitecture and restores bone strength in LPS-induced bone-loss mouse model. Together, our findings indicate that DHA is protective against LPS-induced bone loss through apoptosis induction of osteoclasts via ROS accumulation and the mitochondria-dependent apoptosis pathway. Therefore, DHA may be considered as a new therapeutic candidate for treating inflammatory bone loss. PMID:27031959

  14. Dietary Docosahexaenoic Acid Improves Cognitive Function, Tissue Sparing, and Magnetic Resonance Imaging Indices of Edema and White Matter Injury in the Immature Rat after Traumatic Brain Injury.

    Science.gov (United States)

    Schober, Michelle E; Requena, Daniela F; Abdullah, Osama M; Casper, T Charles; Beachy, Joanna; Malleske, Daniel; Pauly, James R

    2016-02-15

    Traumatic brain injury (TBI) is the leading cause of acquired neurologic disability in children. Specific therapies to treat acute TBI are lacking. Cognitive impairment from TBI may be blunted by decreasing inflammation and oxidative damage after injury. Docosahexaenoic acid (DHA) decreases cognitive impairment, oxidative stress, and white matter injury in adult rats after TBI. Effects of DHA on cognitive outcome, oxidative stress, and white matter injury in the developing rat after experimental TBI are unknown. We hypothesized that DHA would decrease early inflammatory markers and oxidative stress, and improve cognitive, imaging and histologic outcomes in rat pups after controlled cortical impact (CCI). CCI or sham surgery was delivered to 17 d old male rat pups exposed to DHA or standard diet for the duration of the experiments. DHA was introduced into the dam diet the day before CCI to allow timely DHA delivery to the pre-weanling pups. Inflammatory cytokines and nitrates/nitrites were measured in the injured brains at post-injury Day (PID) 1 and PID2. Morris water maze (MWM) testing was performed at PID41-PID47. T2-weighted and diffusion tensor imaging studies were obtained at PID12 and PID28. Tissue sparing was calculated histologically at PID3 and PID50. DHA did not adversely affect rat survival or weight gain. DHA acutely decreased oxidative stress and increased anti-inflammatory interleukin 10 in CCI brains. DHA improved MWM performance and lesion volume late after injury. At PID12, DHA decreased T2-imaging measures of cerebral edema and decreased radial diffusivity, an index of white matter injury. DHA improved short- and long-term neurologic outcomes after CCI in the rat pup. Given its favorable safety profile, DHA is a promising candidate therapy for pediatric TBI. Further studies are needed to explore neuroprotective mechanisms of DHA after developmental TBI. PMID:26247583

  15. The effects of n-3 long-chain polyunsaturated fatty acids on bone formation and growth factors in adolescent boys

    DEFF Research Database (Denmark)

    Damsgaard, C. T.; Mølgaard, C.; Gyldenløve, S. N.;

    2012-01-01

    with docosahexaenoic acid (DHA) status and (ii) affected by fish oil supplementation, in adolescent boys. METHODS: Seventy-eight healthy, slightly overweight 13- to 15-y-old boys were randomly assigned to breads with DHA-rich fish oil (1.1 g/d n-3 LCPUFA) or control for 16 wk. Whole-body bone mineral content (BMC......-1 (IGF-1) during intervention (β = 0.24, P = 0.03, n = 78). DISCUSSION: DHA status and fish oil supplementation were not associated with bone mass or markers of bone formation in adolescent boys, but IGF-1 increased with increasing DHA status.......), bone area (BA), bone mineral density (BMD), plasma osteocalcin, and growth factors were measured at wk 0 and wk 16, as well as diet, physical activity, and n-3 LCPUFA status in erythrocytes. RESULTS: Fish oil strongly increased DHA status (P = 0.0001). No associations were found between DHA status...

  16. ISSFAL Early Career Award Lecture. N-3 Fatty Acids and Membrane Microdomains: From Model Membranes to Lymphocyte Function

    OpenAIRE

    Shaikh, Saame Raza; Teague, Heather

    2012-01-01

    This article summarizes the author's research on fish oil derived n-3 fatty acids, plasma membrane organization and B cell function. We first cover basic model membrane studies that investigated how docosahexaenoic acid (DHA) targeted the organization of sphingolipid-cholesterol enriched lipid microdomains. A key finding here was that DHA had a relatively poor affinity for cholesterol. This work led to a model that predicted DHA acyl chains in cells would manipulate lipid-protein microdomain ...

  17. Induction of lipid oxidation by polyunsaturated fatty acids of marine origin in small intestine of mice fed a high-fat diet

    OpenAIRE

    Hooiveld Guido JEJ; Vink Carolien; Molthoff Jos; Bunschoten Annelies; Kuda Ondrej; Franssen-van Hal Nicole LW; Flachs Pavel; van Schothorst Evert M; Kopecky Jan; Keijer Jaap

    2009-01-01

    Abstract Background Dietary polyunsaturated fatty acids (PUFA), in particular the long chain marine fatty acids docosahexaenoic (DHA) and eicosapentaenoic (EPA), are linked to many health benefits in humans and in animal models. Little is known of the molecular response to DHA and EPA of the small intestine, and the potential contribution of this organ to the beneficial effects of these fatty acids. Here, we assessed gene expression changes induced by DHA and EPA in the wildtype C57BL/6J muri...

  18. A novel 2,6-diisopropylphenyl-docosahexaenoamide conjugate induces apoptosis in T cell acute lymphoblastic leukemia cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Altenburg, Jeffrey D.; Harvey, Kevin A.; McCray, Sharon; Xu, Zhidong [Cellular Biochemistry Laboratory, Methodist Research Institute, Indiana University Health, Indianapolis, IN (United States); Siddiqui, Rafat A., E-mail: rsiddiqu@iuhealth.org [Cellular Biochemistry Laboratory, Methodist Research Institute, Indiana University Health, Indianapolis, IN (United States); Department of Biology, Indiana University-Purdue University, Indianapolis, IN (United States); Department of Medicine, Indiana University School of Medicine, Indianapolis, IN (United States)

    2011-07-29

    Highlights: {yields} 2,6-Diisopropylphenyl-docosahexaenoamide conjugates (DIP-DHA) inhibits the proliferation of T-cell leukemic cell lines. {yields} DIP-DHA resulted in increased activation of caspase-3, and caspase-7. {yields} DIP-DHA significantly downregulated CXCR4 surface expression. -- Abstract: We have previously characterized the effects of 2,6-diisopropylphenyl-docosahexaenoamide (DIP-DHA) conjugates and their analogs on the proliferation and progression of breast cancer cell lines. For this study, we investigated the effects of the DIP-DHA conjugate on 2 representative T cell acute lymphoblastic leukemia (T-ALL) cell lines: CEM and Jurkat. Treatment of both cell lines with DIP-DHA resulted in significantly greater inhibition of proliferation and induction of apoptosis than that of parent compounds, 2,6-diisopropylphenol (DIP) or docosahexaenoate (DHA). Treatment of the cells with DIP-DHA resulted in increased activation of caspase-3, and caspase-7. Furthermore, induction of apoptosis in both cell lines was reversed in the presence of a caspase family inhibitor. Treatment with DIP-DHA reduced mitochondrial membrane potential. These observations suggest that the effects are driven by intrinsic apoptotic pathways. DIP-DHA treatment also downregulated surface CXCR4 expression, an important chemokine receptor involved in cancer metastasis that is highly expressed in both CEM and Jurkat cells. In conclusion, our data suggest that the DIP-DHA conjugate exhibits significantly more potent effects on CEM and Jurkat cells than that of DIP or DHA alone. These conjugates have potential use for treatment of patients with T cell acute lymphoblastic leukemia.

  19. Docosahexaenoic acid signaling modulates cell survival in experimental ischemic stroke penumbra and initiates long-term repair in young and aged rats.

    Directory of Open Access Journals (Sweden)

    Tiffany N Eady

    Full Text Available BACKGROUND: Docosahexaenoic acid, a major omega-3 essential fatty acid family member, improves behavioral deficit and reduces infarct volume and edema after experimental focal cerebral ischemia. We hypothesize that DHA elicits neuroprotection by inducing AKT/p70S6K phosphorylation, which in turn leads to cell survival and protects against ischemic stroke in young and aged rats. METHODS AND RESULTS: Rats underwent 2 h of middle cerebral artery occlusion (MCAo. DHA, neuroprotectin D1 (NPD1 or vehicle (saline was administered 3 h after onset of stroke. Neurological function was evaluated on days 1, 2, 3, and 7. DHA treatment improved functional recovery and reduced cortical, subcortical and total infarct volumes 7 days after stroke. DHA also reduced microglia infiltration and increased the number of astrocytes and neurons when compared to vehicle on days 1 and 7. Increases in p473 AKT and p308 AKT phosphorylation/activation were observed in animals treated with DHA 4 h after MCAo. Activation of other members of the AKT signaling pathway were also observed in DHA treated animals including increases in pS6 at 4 h and pGSK at 24 h. DHA or NPD1 remarkably reduced total and cortical infarct in aged rats. Moreover, we show that in young and aged rats DHA treatment after MCAo potentiates NPD1 biosynthesis. The phosphorylation of p308 AKT or pGSK was not different between groups in aged rats. However, pS6 expression was increased with DHA or NPD1 treatment when compared to vehicle. CONCLUSIONS: We suggest that DHA induces cell survival, modulates the neuroinflammatory response and triggers long term restoration of synaptic circuits. Both DHA and NPD1 elicited remarkable protection in aged animals. Accordingly, activation of DHA signaling might provide benefits in the management of ischemic stroke both acutely as well as long term to limit ensuing disabilities.

  20. Dihydroartemisinin exhibits antitumor activity toward hepatocellular carcinoma in vitro and in vivo.

    Science.gov (United States)

    Zhang, Chris Zhiyi; Zhang, Haitao; Yun, Jingping; Chen, George Gong; Lai, Paul Bo San

    2012-05-01

    Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin isolated from the traditional Chinese herb Artemisia annua L., has been shown to exhibit inhibitory effects on human cancer cells. However, its antitumor ability toward hepatocellular carcinoma (HCC) has not been studied. In this study, we demonstrated that DHA significantly inhibited HCC cell growth in vitro and in vivo via inducing G2/M cell cycle arrest and apoptosis. The induction of p21 and the inhibition of cyclin B and CDC25C contributed to DHA-induced G2/M arrest. DHA-induced apoptosis was associated with mitochondrial membrane depolarization, release of cytochrome c, activation of caspases, and DNA fragmentation. Activation of caspase 9 and caspase 3, but not caspase 8, was detected in DHA-treated cells. Attenuation of apoptosis in cells pretreated with Z-VAD-FMK suggested the involvement of caspase cascade. Furthermore, p53 facilitated apoptosis caused by DHA. Bcl-2 family proteins were also responsible for DHA-induced apoptosis. DHA exposure decreased Mcl-1 expression but increased the levels of Noxa and active Bak. Bak was released from the Mcl-1/Bak complex due to the decline of Mcl-1. Further study revealed that Mcl-1 was rapidly degraded in DHA-treated cells and that DHA-induced apoptosis was largely inhibited by overexpression of Mcl-1 or RNAi-mediated decrease of Bak and Noxa. In a HCC-xenograft mouse model, the intraperitoneal injection of DHA resulted in significant inhibition of HCC xenograft tumors. Taken together, our data, for the first time, demonstrate the potential antitumor activity of DHA in HCC. PMID:22342732