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Sample records for acid auxotrophs constructed

  1. Isolation and Characterization of Unsaturated Fatty Acid Auxotrophs of Streptococcus pneumoniae and Streptococcus mutans▿

    Science.gov (United States)

    Altabe, Silvia; Lopez, Paloma; de Mendoza, Diego

    2007-01-01

    Unsaturated fatty acid (UFA) biosynthesis is essential for the maintenance of membrane structure and function in many groups of anaerobic bacteria. Like Escherichia coli, the human pathogen Streptococcus pneumoniae produces straight-chain saturated fatty acids (SFA) and monounsaturated fatty acids. In E. coli UFA synthesis requires the action of two gene products, the essential isomerase/dehydratase encoded by fabA and an elongation condensing enzyme encoded by fabB. S. pneumoniae lacks both genes and instead employs a single enzyme with only an isomerase function encoded by the fabM gene. In this paper we report the construction and characterization of an S. pneumoniae 708 fabM mutant. This mutant failed to grow in complex medium, and the defect was overcome by addition of UFAs to the growth medium. S. pneumoniae fabM mutants did not produce detectable levels of monounsaturated fatty acids as determined by gas chromatography-mass spectrometry and thin-layer chromatography analysis of the radiolabeled phospholipids. We also demonstrate that a fabM null mutant of the cariogenic organism Streptococcus mutants is a UFA auxotroph, indicating that FabM is the only enzyme involved in the control of membrane fluidity in streptococci. Finally we report that the fabN gene of Enterococcus faecalis, coding for a dehydratase/isomerase, complements the growth of S. pneumoniae fabM mutants. Taken together, these results suggest that FabM is a potential target for chemotherapeutic agents against streptococci and that S. pneumoniae UFA auxotrophs could help identify novel genes encoding enzymes involved in UFA biosynthesis. PMID:17827283

  2. Positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius by use of 5-fluoroorotic acid.

    OpenAIRE

    Kondo, S; Yamagishi, A; Oshima, T

    1991-01-01

    Uracil auxotrophs of Sulfolobus acidocaldarius were positively selected by using 5-fluoroorotic acid. The wild-type strain was unable to grow in medium containing 5-fluoroorotic acid, whereas the mutants grew normally. Positive selection could be done for the auxotrophs. Mutants deficient in orotidine-5'-monophosphate pyrophosphorylase activity were isolated.

  3. Advances in molecular tools for the use of Zygosaccharomyces bailii as host for biotechnological productions and construction of the first auxotrophic mutant.

    Science.gov (United States)

    Dato, Laura; Branduardi, Paola; Passolunghi, Simone; Cattaneo, Davide; Riboldi, Luca; Frascotti, Gianni; Valli, Minoska; Porro, Danilo

    2010-11-01

    The nonconventional yeast Zygosaccharomyces bailii has been proposed as a new host for biotechnological processes due to convenient properties such as its resistance to high sugar concentrations, relatively high temperatures and especially to acidic environments. We describe a series of new expression vectors specific for Z. bailii and the resulting improvements in production levels. By exploiting the sequences of the endogenous plasmid pSB2, 2microm-like multicopy vectors were obtained, giving a fivefold increase in production. A specific integrative vector was developed which led to 100% stability in the absence of selective pressure; a multiple-integration vector was constructed, based on an rRNA gene unit portion cloned and sequenced for this purpose, driving the insertion of up to 80 copies of the foreign construct. Moreover, we show the construction of the first stable auxotrophic mutant of Z. bailii, obtained by targeted gene deletion applied to ZbLEU2. The development of molecular tools for the Z. bailii manipulation has now reached a level that may be compatible with its industrial exploitation; the production of organic acids is a prominent field of application.

  4. The control of ribonucleic acid synthesis in bacteria. The synthesis and stability of ribonucleic acids in relaxed and stringent amino acid auxotrophs of Escherichia coli.

    Science.gov (United States)

    Gray, W J; Midgley, J E

    1972-08-01

    The biosynthesis and stability of various RNA fractions was studied in RC(str) and RC(rel) multiple amino acid auxotrophs of Escherichia coli. In conditions of amino acid deprivation, RC(str) mutants were labelled with exogenous nucleotide bases at less than 1% of the rate found in cultures growing normally in supplemented media. Studies by DNA-RNA hybridization and by other methods showed that, during a period of amino acid withdrawal, not more than 60-70% of the labelled RNA formed in RC(str) mutants had the characteristics of mRNA. Evidence was obtained for some degradation of newly formed 16S and 23S rRNA species to heterogeneous material of lower molecular weight. This led to overestimations of the mRNA content of rapidly labelled RNA from such methods as simple examination of sucrose-density-gradient profiles. In RC(rel) strains the absolute and relative rates of synthesis of the various RNA fractions were not greatly affected. However, the stability of about half of the mRNA fraction was increased in RC(rel) strains during amino acid starvation, giving kinetics of mRNA labelling and turnover that were identical with those found in either RC(str) or RC(rel) strains inhibited by high concentrations of chloramphenicol. Coincidence hybridization techniques showed that the mRNA content of amino acid-starved RC(str) auxotrophs was unchanged from that found in normally growing cells. In contrast, RC(rel) strains deprived of amino acids increased their mRNA content about threefold. In such cultures the mRNA content of accumulating newly formed RNA was a constant 16% by wt.

  5. Identification of auxotrophic mutants of the yeast Kluyveromyces marxianus by non-homologous end joining-mediated integrative transformation with genes from Saccharomyces cerevisiae.

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    Yarimizu, Tohru; Nonklang, Sanom; Nakamura, Junpei; Tokuda, Shuya; Nakagawa, Takaaki; Lorreungsil, Sasithorn; Sutthikhumpha, Surasit; Pukahuta, Charida; Kitagawa, Takao; Nakamura, Mikiko; Cha-Aim, Kamonchai; Limtong, Savitree; Hoshida, Hisashi; Akada, Rinji

    2013-12-01

    The isolation and application of auxotrophic mutants for gene manipulations, such as genetic transformation, mating selection and tetrad analysis, form the basis of yeast genetics. For the development of these genetic methods in the thermotolerant fermentative yeast Kluyveromyces marxianus, we isolated a series of auxotrophic mutants with defects in amino acid or nucleic acid metabolism. To identify the mutated genes, linear DNA fragments of nutrient biosynthetic pathway genes were amplified from Saccharomyces cerevisiae chromosomal DNA and used to directly transform the K. marxianus auxotrophic mutants by random integration into chromosomes through non-homologous end joining (NHEJ). The appearance of transformant colonies indicated that the specific S. cerevisiae gene complemented the K. marxianus mutant. Using this interspecific complementation approach with linear PCR-amplified DNA, we identified auxotrophic mutations of ADE2, ADE5,7, ADE6, HIS2, HIS3, HIS4, HIS5, HIS6, HIS7, LYS1, LYS2, LYS4, LYS9, LEU1, LEU2, MET2, MET6, MET17, TRP3, TRP4 and TRP5 without the labour-intensive requirement of plasmid construction. Mating, sporulation and tetrad analysis techniques for K. marxianus were also established. With the identified auxotrophic mutant strains and S. cerevisiae genes as selective markers, NHEJ-mediated integrative transformation with PCR-amplified DNA is an attractive system for facilitating genetic analyses in the yeast K. marxianus.

  6. Auxotrophic complementation as a selectable marker for stable expression of foreign antigens in Mycobacterium bovis BCG.

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    Borsuk, Sibele; Mendum, Tom A; Fagundes, Michel Quevedo; Michelon, Marcelo; Cunha, Cristina Wetzel; McFadden, Johnjoe; Dellagostin, Odir Antônio

    2007-11-01

    Mycobacterium bovis BCG has the potential to be an effective live vector for multivalent vaccines. However, most mycobacterial cloning vectors rely on antibiotic resistance genes as selectable markers, which would be undesirable in any practical vaccine. Here we report the use of auxotrophic complementation as a selectable marker that would be suitable for use in a recombinant vaccine. A BCG auxotrophic for the amino acid leucine was constructed by knocking out the leuD gene by unmarked homologous recombination. Expression of leuD on a plasmid not only allowed complementation, but also acted as a selectable marker. Removal of the kanamycin resistance gene, which remained necessary for plasmid manipulations in Escherichia coli, was accomplished by two different methods: restriction enzyme digestion followed by re-ligation before BCG transformation, or by Cre-loxP in vitro recombination mediated by the bacteriophage P1 Cre Recombinase. Stability of the plasmid was evaluated during in vitro and in vivo growth of the recombinant BCG in comparison to selection by antibiotic resistance. The new system was highly stable even during in vivo growth, as the selective pressure is maintained, whereas the conventional vector was unstable in the absence of selective pressure. This new system will now allow the construction of potential recombinante vaccine strains using stable multicopy plasmid vectors without the inclusion of antibiotic resistance markers.

  7. Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice.

    Science.gov (United States)

    Ulrich, Ricky L; Amemiya, Kei; Waag, David M; Roy, Chad J; DeShazer, David

    2005-03-14

    Burkholderia mallei is an obligate mammalian pathogen that causes the zoonotic disease glanders. Two live attenuated B. mallei strains, a capsule mutant and a branched-chain amino acid auxotroph, were evaluated for use as vaccines against aerosol-initiated glanders in mice. Animals were aerogenically vaccinated and serum samples were obtained before aerosol challenge with a high-dose (>300 times the LD50) of B. mallei ATCC 23344. Mice vaccinated with the capsule mutant developed a Th2-like Ig subclass antibody response and none survived beyond 5 days. In comparison, the auxotrophic mutant elicited a Th1-like Ig subclass antibody response and 25% of the animals survived for 1 month postchallenge. After a low-dose (5 times the LD50) aerosol challenge, the survival rates of auxotroph-vaccinated and unvaccinated animals were 50 and 0%, respectively. Thus, live attenuated strains that promote a Th1-like Ig response may serve as promising vaccine candidates against aerosol infection with B. mallei.

  8. Chromium induced stress conditions in heterotrophic and auxotrophic strains of Euglena gracilis.

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    Rocchetta, Iara; Mazzuca, Marcia; Conforti, Visitación; Balzaretti, Vilma; del Carmen Ríos de Molina, María

    2012-10-01

    Oxidative stress parameter and antioxidant defense compound as well as enzyme activity were studied in relation to different Cr(VI) concentrations (0, 10, 20, 40 μM) in two strains of Euglena gracilis, one isolated from a polluted river (MAT) and the other acquired from a culture collection (UTEX). Chromium toxicity was measured in the auxotrophic and obligated heterotrophic variants of the two strains. Chromium uptake was higher in auxotrophic cultures, reflected by their higher cell proliferation inhibition and lower IC50 levels compared to heterotrophic ones. In the Cr(VI) treatments a reduction of chlorophyll a and b ratio (Chl a/Chl b) was observed, the ratio of protein to paramylon content was augmented, and total lipid content increased, having the auxotrophic strains the highest values. TBARS content increased significantly only at 40 μM Cr(VI) treatment. Unsaturated fatty acids also increased in the Cr(VI) treatments, with the higher storage lipid (saturated acids) content in the heterotrophic cells. The antioxidant response, such as SOD activity and GSH content, increased with chromium concentration, showing the highest GSH values in the heterotrophic cultures and the SOD enzyme participation in chromium toxicity. The MAT strain had higher IC50 values, higher carbohydrate and saturated acid content, and better response of the antioxidant system than the UTEX one. This strain isolated from the polluted place also showed higher GSH content and SOD activity in control cells and in almost all treated cultures. SOD activity reached a 9-fold increase in both MAT strains. These results suggest that tolerance of MAT strain against Cr(VI) stress is not only related to GSH level and/or biosynthesis capacity but is also related to the participation of the SOD antioxidant enzyme.

  9. Stable expression of Mycobacterium bovis antigen 85B in auxotrophic M. bovis bacillus Calmette-Guérin

    Science.gov (United States)

    Rizzi, Caroline; Peiter, Ana Carolina; Oliveira, Thaís Larré; Seixas, Amilton Clair Pinto; Leal, Karen Silva; Hartwig, Daiane Drawanz; Seixas, Fabiana Kommling; Borsuk, Sibele; Dellagostin, Odir Antônio

    2017-01-01

    BACKGROUND Bovine tuberculosis (TB) is a zoonotic disease caused by Mycobacterium bovis, responsible for causing major losses in livestock. A cost effective alternative to control the disease could be herd vaccination. The bacillus Calmette-Guérin (BCG) vaccine has a limited efficacy against bovine TB, but can improved by over-expression of protective antigens. The M. bovis antigen 85B demonstrates ability to induce protective immune response against bovine TB in animal models. However, current systems for the construction of recombinant BCG expressing multiple copies of the gene result in strains of low genetic stability that rapidly lose the plasmid in vivo. Employing antibiotic resistance as selective markers, these systems also compromise vaccine safety. We previously reported the construction of a stable BCG expression system using auxotrophic complementation as a selectable marker. OBJECTIVES The fundamental aim of this study was to construct strains of M. bovis BCG Pasteur and the auxotrophic M. bovis BCG ΔleuD expressing Ag85B and determine their stability in vivo. METHODS Employing the auxotrophic system, we constructed rBCG strains that expressed M. bovis Ag85B and compared their stability with a conventional BCG strain in mice. Stability was measured in terms of bacterial growth on the selective medium and retention of antigen expression. FINDINGS The auxotrophic complementation system was highly stable after 18 weeks, even during in vivo growth, as the selective pressure and expression of antigen were maintained comparing to the conventional vector. MAIN CONCLUSION The Ag85B continuous expression within the host may generate a stronger and long-lasting immune response compared to conventional systems. PMID:28177046

  10. Generation of Enterobacter sp. YSU auxotrophs using transposon mutagenesis.

    Science.gov (United States)

    Caguiat, Jonathan James

    2014-10-31

    Prototrophic bacteria grow on M-9 minimal salts medium supplemented with glucose (M-9 medium), which is used as a carbon and energy source. Auxotrophs can be generated using a transposome. The commercially available, Tn5-derived transposome used in this protocol consists of a linear segment of DNA containing an R6Kγ replication origin, a gene for kanamycin resistance and two mosaic sequence ends, which serve as transposase binding sites. The transposome, provided as a DNA/transposase protein complex, is introduced by electroporation into the prototrophic strain, Enterobacter sp. YSU, and randomly incorporates itself into this host's genome. Transformants are replica plated onto Luria-Bertani agar plates containing kanamycin, (LB-kan) and onto M-9 medium agar plates containing kanamycin (M-9-kan). The transformants that grow on LB-kan plates but not on M-9-kan plates are considered to be auxotrophs. Purified genomic DNA from an auxotroph is partially digested, ligated and transformed into a pir+ Escherichia coli (E. coli) strain. The R6Kγ replication origin allows the plasmid to replicate in pir+ E. coli strains, and the kanamycin resistance marker allows for plasmid selection. Each transformant possesses a new plasmid containing the transposon flanked by the interrupted chromosomal region. Sanger sequencing and the Basic Local Alignment Search Tool (BLAST) suggest a putative identity of the interrupted gene. There are three advantages to using this transposome mutagenesis strategy. First, it does not rely on the expression of a transposase gene by the host. Second, the transposome is introduced into the target host by electroporation, rather than by conjugation or by transduction and therefore is more efficient. Third, the R6Kγ replication origin makes it easy to identify the mutated gene which is partially recovered in a recombinant plasmid. This technique can be used to investigate the genes involved in other characteristics of Enterobacter sp. YSU or of a

  11. Culture and selection of somatic hybrids using an auxotrophic cell line.

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    Hein, T; Przewoźny, T; Schieder, O

    1983-01-01

    Protoplast fusions between Nicotiana tabacum and N. paniculata and between N. tabacum and N. sylvestris were obtained by polyethylene glycol and Ca(NO3)2 treatment. The protoplasts of one parent originated from cell suspensions, while the protoplasts of the other originated from leaf mesophyll. The heterokaryons were detectable by their intermediate phenotype, namely the green chloroplasts from mesophyll and the dense cytoplasm from suspension cells. They were isolated with micropipettes immediately after fusion using a micromanipulator and were transferred into a protoplast suspension of an auxotrophic cell line serving as a nursery. This mutant is not able to utilize nitrate and had to be supplemented with amino acids. The somatic hybrids were selected by a stepwise reduction of the supplements, which caused the death of the mutant cell colonies, while the autotrophic somatic hybrids continued to grow. The hybrid character of the selected colonies was confirmed by isoenzyme investigations.

  12. Staphylococcus aureus small colony variants show common metabolic features in central metabolism irrespective of the underlying auxotrophism

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    André eKriegeskorte

    2014-10-01

    Full Text Available In addition to the classical phenotype, Staphylococcus aureus may exhibit the small colony-variant (SCV phenotype, which has been associated with chronic, persistent and/or relapsing infections. SCVs are characterized by common phenotypic features such as slow growth, altered susceptibility to antibiotic agents and pathogenic traits based on increased internalization and intracellular persistence. They show frequently auxotrophiesms mainly based on two different mechanisms: (i deficiencies in electron transport as shown for menadione- and/or hemin-auxotrophs and (ii thymidylate biosynthetic-defective SCVs. To get a comprehensive overview of the metabolic differences between both phenotypes, we compared sets of clinically derived menadione-, hemin- and thymidine-auxotrophic SCVs and stable site directed mutants exhibiting the SCV phenotype with their corresponding isogenic parental strains displaying the normal phenotype. Isotopologue profiling and transcriptional analysis of central genes involved in carbon metabolism, revealed large differences between both phenotypes. Labeling experiments with [U-13C6]glucose showed reduced 13C incorporation into aspartate and glutamate from all SCVs irrespective of the underlying auxotrophism. More specifically, these SCVs showed decreased fractions of 13C2-aspartate and glutamate; 13C3-glutamate was not detected at all in the SCVs. In comparison to the patterns in the corresponding experiment with the classical S. aureus phenotype, this indicated a reduced carbon flux via the citric acid cycle in all SCV phenotypes. Indeed, the aconitase-encoding gene (acnA was found down-regulated in all SCV phenotypes under study. In conclusion, all SCV phenotypes including clinical isolates and site-directed mutants displaying the SCV phenotype were characterized by down-regulation of citric acid cycle activity. The common metabolic features in central carbon metabolism found in all SCVs may explain similar

  13. Tunable switch mediated shikimate biosynthesis in an engineered non-auxotrophic Escherichia coli.

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    Gu, Pengfei; Su, Tianyuan; Wang, Qian; Liang, Quanfeng; Qi, Qingsheng

    2016-01-01

    Shikimate is a key intermediate in the synthesis of neuraminidase inhibitors. Compared with traditional methods, microbial production of shikimate has the advantages of environmental friendliness, low cost, feed stock renewability, and product selectivity and diversity. Despite these advantages, shikimate kinase I and II respectively encoded by aroK and aroL are inactivated in most shikimate microbial producers, thus requiring the addition of aromatic compounds during the fermentation process. To overcome this problem, we constructed a non-auxotrophic, shikimate-synthesising strain of Escherichia coli. By inactivation of repressor proteins, blocking of competitive pathways and overexpression of key enzymes, we increased the shikimate production of wild-type E. coli BW25113 to 1.73 g/L. We then designed a tunable switch that can conditionally decrease gene expression and substituted it for the original aroK promoters. Expression of aroK in the resulting P-9 strain was maintained at a high level during the growth phase and then reduced at a suitable time by addition of an optimal concentration of inducer. In 5-L fed-batch fermentation, strain P-9 produced 13.15 g/L shikimate without the addition of any aromatic compounds. The tunable switch developed in this study is an efficient tool for regulating indispensable genes involved in critical metabolic pathways.

  14. Dependence of mutation spectrum on physiological state of Pen. Chrysogenum cells. Auxotrophic mutants induced with UV rays at various stages of DNA replication

    Energy Technology Data Exchange (ETDEWEB)

    Zakharova, G.M.; Bartoshevich, Yu.Eh.; Lebed, Eh.S.; Domracheva, A.G.

    1981-01-01

    The effect of UV-rays on non-activated and activated conidia of penicillin producers Pen, chryrogenum, strain 39, has been studied. It has been established that 2 doses of UV-rays - 4000 and 6000 erg/mm/sup 2/ - induce in non-activated conidia approximately equal quantity of mutations with broken synthesis of amino acids and nitrous bases of nucleic acids. The spectrum of auxotrophic mutations induced during the DNA replication changes depending on the mutagen dose and replication stage. No distinct periodicity in the mutation yield has been observed. Schemes of the induction of mutations during the DNA replication under the effect of both doses of UV-rays have been made.

  15. Acetic acid inhibits nutrient uptake in Saccharomyces cerevisiae: auxotrophy confounds the use of yeast deletion libraries for strain improvement.

    Science.gov (United States)

    Ding, Jun; Bierma, Jan; Smith, Mark R; Poliner, Eric; Wolfe, Carole; Hadduck, Alex N; Zara, Severino; Jirikovic, Mallori; van Zee, Kari; Penner, Michael H; Patton-Vogt, Jana; Bakalinsky, Alan T

    2013-08-01

    Acetic acid inhibition of yeast fermentation has a negative impact in several industrial processes. As an initial step in the construction of a Saccharomyces cerevisiae strain with increased tolerance for acetic acid, mutations conferring resistance were identified by screening a library of deletion mutants in a multiply auxotrophic genetic background. Of the 23 identified mutations, 11 were then introduced into a prototrophic laboratory strain for further evaluation. Because none of the 11 mutations was found to increase resistance in the prototrophic strain, potential interference by the auxotrophic mutations themselves was investigated. Mutants carrying single auxotrophic mutations were constructed and found to be more sensitive to growth inhibition by acetic acid than an otherwise isogenic prototrophic strain. At a concentration of 80 mM acetic acid at pH 4.8, the initial uptake of uracil, leucine, lysine, histidine, tryptophan, phosphate, and glucose was lower in the prototrophic strain than in a non-acetic acid-treated control. These findings are consistent with two mechanisms by which nutrient uptake may be inhibited. Intracellular adenosine triphosphate (ATP) levels were severely decreased upon acetic acid treatment, which likely slowed ATP-dependent proton symport, the major form of transport in yeast for nutrients other than glucose. In addition, the expression of genes encoding some nutrient transporters was repressed by acetic acid, including HXT1 and HXT3 that encode glucose transporters that operate by facilitated diffusion. These results illustrate how commonly used genetic markers in yeast deletion libraries complicate the effort to isolate strains with increased acetic acid resistance.

  16. Feasibility of biohydrogen production from tofu wastewater with glutamine auxotrophic mutant of Rhodobacter sphaeroides

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, G.H.; Wang, L.; Kang, Z.H. [School of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping road, Shanghai 200092 (China)

    2010-12-15

    NH{sub 4}{sup +}, which is normally the integrant in organic wastewater, such as Tofu wastewater, is an inhibitor to hydrogen production by anoxygenic phototrophic bacterium. In order to release inhibition of NH{sub 4}{sup +} to biohydrogen generation by Rhodobacter sphaeroides, a glutamine auxotrophic mutant R. sphaeroides TJ-0803 was obtained by mutagenizing with ethyl methane sulfonate. The mutant could generate biohydrogen efficiently in the medium with high NH{sub 4}{sup +} concentration, because the inhibition of NH{sub 4}{sup +} to nitrogenase was released. Under suitable conditions, TJ-0803 could effectively produce biohydrogen from tofu wastewater, which commonly containing 50-60 mg L{sup -1} NH{sub 4}{sup +}, and the generation rate was increased by more than 100% compared with that from wild-type R. sphaeroides. (author)

  17. Construction of a cyanobacterium synthesizing cyclopropane fatty acids.

    Science.gov (United States)

    Machida, Shuntaro; Shiraiwa, Yoshihiro; Suzuki, Iwane

    2016-09-01

    Microalgae have received much attention as a next-generation source of biomass energy. However, most of the fatty acids (FAs) from microalgae are multiply unsaturated; thus, the biofuels derived from them are fluid, but vulnerable to oxidation. In this study, we attempted to synthesize cyclopropane FAs in the cyanobacterium Synechocystis sp. PCC 6803 by expressing the cfa gene for cyclopropane FA synthase from Escherichia coli with the aim of producing FAs that are fluid and stable in response to oxidization. We successfully synthesized cyclopropane FAs in Synechocystis with a yield of ~30% of total FAs. Growth of the transformants was altered, particularly at low temperatures, but photosynthesis and respiration were not significantly affected. C16:1(∆9) synthesis in the desA(-)/desD(-) strain by expression of the desC2 gene for sn-2 specific ∆9 desaturase positively affected growth at low temperatures via promotion of various cellular processes, with the exceptions of photosynthesis and respiration. Estimation of the apparent activities of desaturases suggested that some acyl-lipid desaturases might recognize the lipid side chain.

  18. Recombinant host cells and nucleic acid constructs encoding polypeptides having cellulolytic enhancing activity

    Energy Technology Data Exchange (ETDEWEB)

    Schnorr, Kirk; Kramer, Randall

    2017-03-28

    The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

  19. Directed arginine deiminase evolution for efficient inhibition of arginine-auxotrophic melanomas.

    Science.gov (United States)

    Cheng, Feng; Zhu, Leilei; Lue, Hongqi; Bernhagen, Jürgen; Schwaneberg, Ulrich

    2015-02-01

    Arginine deiminase (ADI) is a therapeutic protein for cancer therapy of arginine-auxotrophic tumors. However, ADI's application as anticancer drug is hampered by its low activity for arginine under physiological conditions mainly due to its high "K M" (S₀.₅) values which are often 1 magnitude higher than the arginine concentration in blood (0.10-0.12 mM arginine in human plasma). Previous evolution campaigns were directed by us with the aim of boosting activity of PpADI (ADI from Pseudomonas plecoglossicida, k cat = 0.18 s(-1); S₀.₅ = 1.30 mM), and yielded variant M6 with slightly reduced S₀.₅ values and enhanced k cat (S₀.₅ = 0.81 mM; k cat = 11.64 s(-1)). In order to further reduce the S₀.₅ value and to increase the activity of PpADI at physiological arginine concentration, a more sensitive screening system based on ammonia detection in 96-well microtiter plate to reliably detect ≥0.005 mM ammonia was developed. After screening ~5,500 clones with the ammonia detection system (ADS) in two rounds of random mutagenesis and site-directed mutagenesis, variant M19 with increased k cat value (21.1 s(-1); 105.5-fold higher compared to WT) and reduced S₀.₅ value (0.35 mM compared to 0.81 mM (M6) and 1.30 mM (WT)) was identified. Improved performance of M19 was validated by determining IC₅₀ values for two melanoma cell lines. The IC₅₀ value for SK-MEL-28 dropped from 8.67 (WT) to 0.10 (M6) to 0.04 μg/mL (M19); the IC₅₀ values for G361 dropped from 4.85 (WT) to 0.12 (M6) to 0.05 μg/mL (M19).

  20. Immunization of olive flounder (Paralichthys olivaceus) with an auxotrophic Edwardsiella tarda mutant harboring the VHSV DNA vaccine.

    Science.gov (United States)

    Choi, Seung Hyuk; Kim, Min Sun; Kim, Ki Hong

    2012-09-01

    The aims of the present study were to find more powerful promoter for DNA vaccines in olive flounder (Paralichthys olivaceus) and to evaluate the availability of the auxotrophic Edwardsiella tarda mutant (Δalr Δasd E. tarda) as a delivery vehicle for DNA vaccine against VHSV in olive flounder. The marine medaka (Oryzias dancena) β-actin promoter was clearly stronger than cytomegalovirus (CMV) promoter when the vectors were transfected to Epithelioma papulosum cyprini (EPC) cells or injected into the muscle of olive flounder, suggesting that marine medaka β-actin promoter would be more appropriate promoter for DNA vaccines in olive flounder than CMV promoter. Olive flounder immunized with the Δalr Δasd E. tarda harboring viral hemorrhagic septicemia virus (VHSV) DNA vaccine vector driven by the marine medaka β-actin promoter showed significantly higher serum neutralization titer and higher survival rates against challenge with VHSV than fish immunized with the bacteria carrying VHSV DNA vaccine vector driven by CMV promoter. These results indicate that auxotrophic E. tarda mutant harboring marine medaka β-actin promoter-driven DNA vaccine vectors would be a potential system for prophylactics of infectious diseases in olive flounder.

  1. Pre-clinical development of BCG.HIVA(CAT, an antibiotic-free selection strain, for HIV-TB pediatric vaccine vectored by lysine auxotroph of BCG.

    Directory of Open Access Journals (Sweden)

    Narcís Saubi

    Full Text Available In the past, we proposed to develop a heterologous recombinant BCG prime-recombinant modified vaccinia virus Ankara (MVA boost dual pediatric vaccine platform against transmission of breast milk HIV-1 and Mycobacterium tuberculosis (Mtb. In this study, we assembled an E. coli-mycobacterial shuttle plasmid pJH222.HIVA(CAT expressing HIV-1 clade A immunogen HIVA. This shuttle vector employs an antibiotic resistance-free mechanism based on Operator-Repressor Titration (ORT system for plasmid selection and maintenance in E. coli and lysine complementation in mycobacteria. This shuttle plasmid was electroporated into parental lysine auxotroph (safer strain of BCG to generate vaccine BCG.HIVA(CAT. All procedures complied with Good Laboratory Practices (GLPs. We demonstrated that the episomal plasmid pJH222.HIVA(CAT was stable in vivo over a 20-week period, and genetically and phenotypically characterized the BCG.HIVA(CAT vaccine strain. The BCG.HIVA(CAT vaccine in combination with MVA.HIVA induced HIV-1- and Mtb-specific interferon γ-producing T-cell responses in newborn and adult BALB/c mice. On the other hand, when adult mice were primed with BCG.HIVA(CAT and boosted with MVA.HIVA.85A, HIV-1-specific CD8(+ T-cells producing IFN-γ, TNF-α, IL-2 and CD107a were induced. To assess the biosafety profile of BCG.HIVA(CAT-MVA.HIVA regimen, body mass loss of newborn mice was monitored regularly throughout the vaccination experiment and no difference was observed between the vaccinated and naïve groups of animals. Thus, we demonstrated T-cell immunogenicity of a novel, safer, GLP-compatible BCG-vectored vaccine using prototype immunogen HIVA. Second generation immunogens derived from HIV-1 as well as other major pediatric pathogens can be constructed in a similar fashion to prime protective responses soon after birth.

  2. [Construction and composition diversity of a lactic acid bacterial community SFC-2].

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    Gao, Li-juan; Wang, Xiao-fen; Yang, Hong-yan; Li, Xian-mei; Cui, Zong-jun

    2007-05-01

    To obtain the community that could accelerate the fermentation of the air-dried crop straws, a lactic acid bacteria community SFC-2 was constructed from the natural fermented products of the corn straw and rice straw by continuous restricted subcultivation in the MRS-S broth. The SFC-2 could lower the pH of the broth quickly, and produce high amount of lactic acid. The microbial composition of the SFC-2 became stable from the 25th generation according to the results of denaturing gradient gel electrophoresis (DGGE). The pH value of the SFC-2 dropped to 3.8 and the amount of lactic acid reached 10.64 mg x mL(-1) after 12 h cultivation in the broth, which contained 64% L-(+) lactic acid. Using morphological methods, four strains were isolated from the SFC-2 and they all belonged to Lactobacillus. The closest species were L. fermentum, L. plantarum, L. paracasei and L. paracasei sub sp., respectively. At the same time, the composition of the SFC-2 was also analyzed by constructing 16S rDNA clone library. The results of 16S rDNA sequencing showed that the closest species of seven clones were mainly L. fermentum, L. plantarum and L. paracasei. In clone libraries, 76.3% belonged to L. fermentum, 20.3% belonged to L. plantarum and 3.4% belonged to L. paracasei.

  3. 黑曲霉菌PyrG缺陷株的建立%CONSTRUCTION OF PYRG AUXOTROPHIC Aspergillus niger STRAIN

    Institute of Scientific and Technical Information of China (English)

    刘钟滨; DavidJ.Jeenes; 等

    2001-01-01

    运用紫外线照射致突变方法建立了黑曲霉菌ATCC 12049,13496和N402等3种菌株的乳清酸核苷-5′-磷酸脱羧酶基因(pyrG)缺陷株.其中ATCC 13496是一种蛋白酶缺陷株.含黑曲霉菌pyrG基因的重组质粒pY 1.2可使它们发生转化,成为Pyr+,转化效率约为8~40转化子/μgDNA.这些pyrG缺陷株将可被用作基因工程的受体菌.

  4. Selection of nitrogen-fixing deficient Burkholderia vietnamiensis strains by cystic fibrosis patients: involvement of nif gene deletions and auxotrophic mutations.

    Science.gov (United States)

    Menard, Aymeric; Monnez, Claire; Estrada de Los Santos, Paulina; Segonds, Christine; Caballero-Mellado, Jesus; Lipuma, John J; Chabanon, Gerard; Cournoyer, Benoit

    2007-05-01

    Burkholderia vietnamiensis is the third most prevalent species of the Burkholderia cepacia complex (Bcc) found in cystic fibrosis (CF) patients. Its ability at fixing nitrogen makes it one of the main Bcc species showing strong filiations with environmental reservoirs. In this study, 83% (29 over 35) of the B. vietnamiensis CF isolates and 100% of the environmental ones (over 29) were found expressing the dinitrogenase complex (encoded by the nif cluster) which is essential in N(2) fixation. Among the deficient strains, two were found growing with ammonium chloride suggesting that they were defective in N(2) fixation, and four with amino acids supplements suggesting that they were harbouring auxotrophic mutations. To get insights about the genetic events that led to the emergence of the N(2)-fixing defective strains, a genetic analysis of B. vietnamiensis nitrogen-fixing property was undertaken. A 40-kb-long nif cluster and nif regulatory genes were identified within the B. vietnamiensis strain G4 genome sequence, and analysed. Transposon mutagenesis and nifH genetic marker exchanges showed the nif cluster and several other genes like gltB (encoding a subunit of the glutamate synthase) to play a key role in B. vietnamiensis ability at growing in nitrogen-free media. nif cluster DNA probings of restricted genomic DNA blots showed a full deletion of the nif cluster for one of the N(2)-fixing defective strain while the other one showed a genetic organization similar to the one of the G4 strain. For 17% of B. vietnamiensis clinical strains, CF lungs appeared to have favoured the selection of mutations or deletions leading to N(2)-fixing deficiencies.

  5. Construction

    Science.gov (United States)

    2002-01-01

    Harbor Deepening Project, Jacksonville, FL Palm Valley Bridge Project, Jacksonville, FL Rotary Club of San Juan, San Juan, PR Tren Urbano Subway...David. What is nanotechnology? What are its implications for construction?, Foresight/CRISP Workshop on Nanotechnology, Royal Society of Arts

  6. Supramolecular Coordination Assemblies Constructed From Multifunctional Azole-Containing Carboxylic Acids

    Directory of Open Access Journals (Sweden)

    Yuheng Deng

    2010-05-01

    Full Text Available This paper provides a brief review of recent progress in the field of metal coordination polymers assembled from azole-containing carboxylic acids and gives a diagrammatic summary of the diversity of topological structures in the resulting infinite metal-organic coordination networks (MOCNs. Azole-containing carboxylic acids are a favorable kind of multifunctional ligand to construct various metal complexes with isolated complexes and one, two and three dimensional structures, whose isolated complexes are not the focus of this review. An insight into the topology patterns of the infinite coordination polymers is provided. Analyzed topologies are compared with documented topologies and catalogued by the nature of nodes and connectivity pattern. New topologies which are not available from current topology databases are described and demonstrated graphically.

  7. Assessment of the microbial community in a constructed wetland that receives acid coal mine drainage

    Energy Technology Data Exchange (ETDEWEB)

    Nicomrat, D.; Dick, W.A.; Tuovinen, O.H. [Ohio State University, Columbus, OH (United States)

    2006-01-15

    Constructed wetlands are used to treat acid drainage from surface or underground coal mines. However, little is known about the microbial communities in the receiving wetland cells. The purpose of this work was to characterize the microbial population present in a wetland that was receiving acid coal mine drainage (AMD). Samples were collected from the oxic sediment zone of a constructed wetland cell in southeastern Ohio that was treating acid drainage from an underground coal mine seep. Samples comprised Fe(Ill) precipitates and were pretreated with ammonium oxalate to remove interfering iron, and the DNA was extracted and purified by agarose gel electrophoresis prior to amplification of portions of the 16S rRNA gene. Amplified products were separated by denaturing gradient gel electrophoresis and DNA from seven distinct bands was excised from the gel and sequenced. The sequences were matched to sequences in the GenBank bacterial 16S rDNA database. The DNA in two of the bands yielded matches with Acidithiobacillus ferrooxidans and the DNA in each of the remaining five bands was consistent with one of the following microorganisms: Acidithiobacillus thiooxidans, strain TRA3-20 (a eubacterium), strain BEN-4 (an arsenite-oxidizing bacterium), an Alcaligenes sp., and a Bordetella sp. Low bacterial diversity in these samples reflects the highly inorganic nature of the oxic sediment layer where high abundance of iron- and sulfur-oxidizing bacteria would be expected. The results we obtained by molecular methods supported our findings, obtained using culture methods, that the dominant microbial species in an acid receiving, oxic wetland are A. thiooxidans and A. ferrooxidans.

  8. Production of fired construction brick from high sulfate-containing fly ash with boric acid addition.

    Science.gov (United States)

    Başpinar, M Serhat; Kahraman, Erhan; Görhan, Gökhan; Demir, Ismail

    2010-01-01

    The increase of power plant capacity has led to the production of an increasing amount of fly ash that causes high environmental impact in Turkey. Some of the fly ash is utilized within the fired brick industry but high sulfate-containing fly ash creates severe problems during sintering of the fired brick. This study attempted to investigate the potential for converting high sulfate-containing fly ash into useful material for the construction industry by the addition of boric acid. The chemical and mineralogical composition of fly ash and clay were investigated. Boric acid (H(3)BO(3)) was added to fly ash-clay mixtures with up to 5 wt.%. Six different series of test samples were produced by uniaxial pressing. The samples were fired at the industrial clay-brick firing temperatures of 800, 900 and 1000 degrees C. The microstructures of the fired samples were investigated by scanning electron microscopy and some physical and mechanical properties were measured. It was concluded that the firing at conventional brick firing temperature of high sulfate fly ash without any addition of boric acid resulted in very weak strength bricks. The addition of boric acid and clay simultaneously to the high sulfate- containing fly ash brick dramatically increased the compressive strength of the samples at a firing temperature of 1000 degrees C by modifying the sintering behaviour of high sulfate fly ash.

  9. Construction of HA-1-DC Nucleic-acid Vaccine and Induction of Specific Cytotoxic T Lymphocytes

    Institute of Scientific and Technical Information of China (English)

    WANG Yaya; ZHANG Donghua; HU Jinmei; LIU Wenli; ZHOU hongsheng; ZHANG Lu; LIU Dan; HUANG Zhenqian; TAN Huo

    2007-01-01

    An HA-1-DC nucleic-acid vaccine was constructed to induce anti-leukemia effect after hematopoietic stem cell transplantation (HSCT). DCs were generated from HSCT donors in vitro, and its immunologic activity was assayed by using flow cytometry and mixed lymphocytes reaction.HA-1 gene was electroporated into the cultured DCs to construct a DC nucleic-acid vaccine. After transfection for 48 h, the expression of HA-1 protein could be detected by using Western blot. The DCs were cultured with syngenic lymphocytes to induce specific cytotoxic T lymphocytes (CTLs).The cytoxicity of the CTLs was detected by LDH assay. The results showed that The DCs derived from peripheral blood monocytes (PBMCs) expressed the phenotype of DCs, and were effective in stimulating proliferation of the allogenic lymphocytes. After electroporating for 48-h, HA-1 protein was detected by using Western blot. The cytotoxity of inducing CTLs was higher than the control group. It was suggested that minor histocompatibility antigen HA-1 could be considered as a target of immunotherapy against leukemia after HSCT.

  10. Codes in the codons: construction of a codon/amino acid periodic table and a study of the nature of specific nucleic acid-protein interactions.

    Science.gov (United States)

    Benyo, B; Biro, J C; Benyo, Z

    2004-01-01

    The theory of "codon-amino acid coevolution" was first proposed by Woese in 1967. It suggests that there is a stereochemical matching - that is, affinity - between amino acids and certain of the base triplet sequences that code for those amino acids. We have constructed a common periodic table of codons and amino acids, where the nucleic acid table showed perfect axial symmetry for codons and the corresponding amino acid table also displayed periodicity regarding the biochemical properties (charge and hydrophobicity) of the 20 amino acids and the position of the stop signals. The table indicates that the middle (2/sup nd/) amino acid in the codon has a prominent role in determining some of the structural features of the amino acids. The possibility that physical contact between codons and amino acids might exist was tested on restriction enzymes. Many recognition site-like sequences were found in the coding sequences of these enzymes and as many as 73 examples of codon-amino acid co-location were observed in the 7 known 3D structures (December 2003) of endonuclease-nucleic acid complexes. These results indicate that the smallest possible units of specific nucleic acid-protein interaction are indeed the stereochemically compatible codons and amino acids.

  11. Synthesis, crystal structure and optical property of three coordination polymer constructed from m-phenylenediacrylate acid

    Energy Technology Data Exchange (ETDEWEB)

    Xiao, YiFan; Wang, TingTing; Zeng, HePing, E-mail: hpzeng@scut.edu.cn

    2015-01-15

    Three new complexes of the wholly deprotonated flexible ligand m-phenylenediacrylate acid (H{sub 2}mpda), namely, [Zn (mpda) (H{sub 2}O)] [1], [Pr{sub 2}(mpda){sub 2}(H{sub 2}O){sub 2}(CHOO){sub 2}] [2], and [Mn{sub 2}(mpda){sub 2} (H{sub 2}O){sub 4}] [3], were synthesized by the solvothermal reaction. The complexes were characterized by IR spectroscopy, thermogravimetric analysis, and X-ray single-crystal diffraction. A mixed sample [Nd(mpda)(H{sub 2}O)(CHOO)·Pr(mpda)(H{sub 2}O)(CHOO)] [2{sup ]} was also synthesized and studied. Compounds [1] and [2] exhibit a selective sensing function with respect to acetone, and they are a potential luminescent sensory material for the selective detection of Zn{sup 2+} and Ni{sup 2+} ions, respectively. Furthermore, the fluorescent changes of compound [1] upon the addition of cations (Fe{sup 3+} and Zn{sup 2+}, Bi{sup 2+} and Zn{sup 2+}) are utilized to construct two logic gates at the molecular level. - Graphical abstract: Three new complexes of flexible ligand m-phenylenedicarboxylic acid were synthesized by solvothermal reaction. Three metal−organic frameworks exhibiting 1D, 2D, 3D structures, respectively. - Highlights: • Three new complexes of m-phenylenedicarboxylic acid were synthesized. • Three metal−organic frameworks exhibiting 1D, 2D, 3D structures, respectively. • [1] and [2] exhibits a very high quenching effect with acetone. • [1] and [2] are a potential luminescent sensory material for Zn{sup 2+}, Ni{sup 2+} ions. • Two logic gates have been constructed using compound [1].

  12. Bacterial phylogenetic diversity in a constructed wetland system treating acid coal mine drainage

    Energy Technology Data Exchange (ETDEWEB)

    Nicorarat, D.; Dick, W.A.; Dopson, M.; Tuovinen, O.H. [Ohio State University, Columbus, OH (USA)

    2008-02-15

    Microorganisms in acid mine drainage are typically acidophiles that mediate the oxidation of reduced compounds of iron and sulfur. However, microbial populations in wetland systems constructed to treat acid mine drainage are not well characterized. This study was to analyze bacterial diversity, using cultivation-independent molecular ecological techniques, in a constructed wetland that received acid drainage from an abandoned underground coal mine. DNA was purified from Fe(III)-precipitates from the oxidized surface zone of wetland sediments and 16S rRNA gene sequences were amplified and cloned. A total of 200 clones were analyzed by restriction fragment length polymorphism (RFLP) and 77 unique RFLP patterns were obtained with four restriction enzymes. Of these patterns, 30 most dominant unique clones were selected for sequencing of their 16S rRNA genes. Half of these 30 clones could be matched with autotrophic iron- and sulfur-oxidizing bacteria (Acidithiohacillus ferrooxidans and Acidithiobacillus thiooxidans). Several clones also formed a clade with heterotrophic iron-oxidizing bacteria (TRA2-10, TRA3-20, and TRA5-3) and heterotrophic bacteria (Stenotrophomas maltophilia, Bordetella spp., Alcalgenes sp., Alcaligenesfaecalis, and Alcaligenes xylosoxidans). Approximately 40% and 35% of the analyzed RFLP restriction patterns were consistent with A. ferrooxidans and A. thiooxidans, respectively. The relatively high frequency of acidithiobacilli is consistent with the chemical and physical characteristics of this site i.e., continuous, abundant supply of reduced iron and sulfur compounds, pH 3-4, ambient temperature, and limited organics originating from the coal seam and from vegetation or soil surrounding the inlet channel to the wetland.

  13. Design, synthesis and docking studies of novel dipeptidyl boronic acid proteasome inhibitors constructed from αα- and αβ-amino acids.

    Science.gov (United States)

    Shi, Jingmiao; Lei, Meng; Wu, Wenkui; Feng, Huayun; Wang, Jia; Chen, Shanshan; Zhu, Yongqiang; Hu, Shihe; Liu, Zhaogang; Jiang, Cheng

    2016-04-15

    A series of novel dipeptidyl boronic acid proteasome inhibitors constructed from αα- and αβ-amino acids were designed and synthesized. Their structures were elucidated by (1)H NMR, (13)C NMR, LC-MS and HRMS. These compounds were evaluated for their β5 subunit inhibitory activities of human proteasome. The results showed that dipeptidyl boronic acid inhibitors composed of αα-amino acids were as active as bortezomib. Interestingly, the activities of those derived from αβ-amino acids lost completely. Of all the inhibitors, compound 22 (IC50=4.82 nM) was the most potent for the inhibition of proteasome activity. Compound 22 was also the most active against three MM cell lines with IC50 values less than 5 nM in inhibiting cell growth assays. Molecular docking studies displayed that 22 fitted very well in the β5 subunit active pocket of proteasome.

  14. Construction of the Core of Pseudolaric Acid A and Mechanistic Studies on Intramolecular [4+3] Cycloaddition

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    This paper describes the construction of hemiacetal 2, the core of pseudolaric acid A via oxidative cleavage of acetonide 6 or 7 and enolization-hemiacetalization of aldehyde 8. A plausible general mechanism for the intramolecular [4+3] cycloaddition of sulfoxide 4 to adduct 3 is suggested.

  15. Novel one-dimensional lanthanide acrylic acid complexes: an alternative chain constructed by hydrogen bonding

    Science.gov (United States)

    Li, Hui; Hu, Chang Wen

    2004-12-01

    Novel one-dimensional (1D) chains of three lanthanide complexes La(L 1) 3(CH 3OH)]·CH 3OH (L 1=(E)-3-(2-hydroxyl-phenyl)-acrylic acid) 1, La(L 2) 3(H 2O) 2]·2.75H 2O (L 2=(E)-3-(3-hydroxyl-phenyl)-acrylic acid) 2, and La(L 3) 3(CH 3OH) 2(H 2O)]·CH 3OH (L 3=(E)-3-(4-hydroxyl-phenyl)-acrylic acid) 3 are reported. The crystal structure data are as follows for 1: C 29H 29LaO 11, monoclinic, P2 1/ n, a=15.4289(12) Å, b=7.9585(6) Å, c=23.041(2) Å, β=99.657(2)°, Z=4, R1=0.0637, w R2=0.0919; for 2: C 27H 30.50LaO 13.75, triclinic, P-1, a=8.4719(17) Å, b=13.719(3) Å, c=14.570(3) Å, α=62.19(3)°, β=99.657(2)°, γ=78.22(3)°, Z=2, R1=0.0384, w R2=0.0820; and for 3: C 30H 35LaO 13, monoclinic, P2(1)/ c, a=9.5667(6) Å, b=24.3911(15) Å, c=14.0448(9) Å, β=109.245(2)°, Z=4, R1=0.0374, w R2=0.0630. All the three structure data were collected using graphite monochromated molybdenum Kα radiation and refined using full-matrix least-squares techniques on F 2. These structures show that four kinds of the carboxylato bridge modes are included in these chains to link the La(III) ions. It is the first time that it has been found that the intra-chain hydrogen bonding can construct an alternative chain even, when the coordination bridge mode is the same along the chain (complex 2). There are 2D and 3D hydrogen bonding in the crystal lattices of complexes 1- 3.

  16. Predicting Three-Dimensional Conformations of Peptides Constructed of Only Glycine, Alanine, Aspartic Acid, and Valine

    Science.gov (United States)

    Oda, Akifumi; Fukuyoshi, Shuichi

    2015-06-01

    The GADV hypothesis is a form of the protein world hypothesis, which suggests that life originated from proteins (Lacey et al. 1999; Ikehara 2002; Andras 2006). In the GADV hypothesis, life is thought to have originated from primitive proteins constructed of only glycine, alanine, aspartic acid, and valine ([GADV]-proteins). In this study, the three-dimensional (3D) conformations of randomly generated short [GADV]-peptides were computationally investigated using replica-exchange molecular dynamics (REMD) simulations (Sugita and Okamoto 1999). Because the peptides used in this study consisted of only 20 residues each, they could not form certain 3D structures. However, the conformational tendencies of the peptides were elucidated by analyzing the conformational ensembles generated by REMD simulations. The results indicate that secondary structures can be formed in several randomly generated [GADV]-peptides. A long helical structure was found in one of the hydrophobic peptides, supporting the conjecture of the GADV hypothesis that many peptides aggregated to form peptide multimers with enzymatic activity in the primordial soup. In addition, these results indicate that REMD simulations can be used for the structural investigation of short peptides.

  17. Predicting three-dimensional conformations of peptides constructed of only glycine, alanine, aspartic acid, and valine.

    Science.gov (United States)

    Oda, Akifumi; Fukuyoshi, Shuichi

    2015-06-01

    The GADV hypothesis is a form of the protein world hypothesis, which suggests that life originated from proteins (Lacey et al. 1999; Ikehara 2002; Andras 2006). In the GADV hypothesis, life is thought to have originated from primitive proteins constructed of only glycine, alanine, aspartic acid, and valine ([GADV]-proteins). In this study, the three-dimensional (3D) conformations of randomly generated short [GADV]-peptides were computationally investigated using replica-exchange molecular dynamics (REMD) simulations (Sugita and Okamoto 1999). Because the peptides used in this study consisted of only 20 residues each, they could not form certain 3D structures. However, the conformational tendencies of the peptides were elucidated by analyzing the conformational ensembles generated by REMD simulations. The results indicate that secondary structures can be formed in several randomly generated [GADV]-peptides. A long helical structure was found in one of the hydrophobic peptides, supporting the conjecture of the GADV hypothesis that many peptides aggregated to form peptide multimers with enzymatic activity in the primordial soup. In addition, these results indicate that REMD simulations can be used for the structural investigation of short peptides.

  18. [Constructing recombinant plasmid pSH-CUP and knockout of acid trehalase gene in baker's yeast].

    Science.gov (United States)

    He, Dongqin; Xiao, Dongguang; Lv, Ye

    2008-02-01

    The ATH1 gene encoded acid trehalase in Saccharomyces cerevisiae. The gene disruption cassette combined the heterologous dominant kan(r) resistance marker with a Cre/loxP-mediated marker removal procedure. The gene disruption cassette was produced by PCR using the same long oligonucleotides comprising 50 nucleotides that annealed to sites upstream or downstream of the genomic target sequence to be deleted. After transformation of the linear disruption cassettes with a Cre/loxP-mediated marker into the cells of Saccharomyces cerevisiae BY-6, selected transformants were checked by PCR for correct the integration of the cassette and concurrent deletion of the chromosomal target sequence. The copper-resistance gene (CUP1-MT1) was cloned into pSH47, which yielded pSH-CUP. The recombinant plasmid pSH-CUP was transformed into the cells of Saccharomyces cerevisiae BY-6(delta ATH1, G418(r)), and transformants were selected for copper resistance. Upon expression of the Cre recombinase results in removal of the kan(r) gene, leaving behind a single loxP site at the chromosomal locus. Construction of the recombinant plasmid pSH-CUP avoided inserting non-yeast gene and made the loxP - kanMX - loxP gene disruption cassette more conventional for eukaryotic organism gene disruption.

  19. Vitamin B12, folic acid, ferritin and haematological variables among Thai construction site workers in urban Bangkok.

    Science.gov (United States)

    Tungtrongchitr, R; Pongpaew, P; Phonrat, B; Chanjanakitskul, S; Paksanont, S; Migasena, P; Schelp, F P

    1995-01-01

    Serum vitamin B12, folic acid, ferritin and haematological variables were investigated in eighty-seven male and nineteen female construction site workers in Bangkok. Haemoglobin concentration, haematocrit and MCHC were found to be higher in male than in female workers. Serum ferritin was slightly higher in males than in females. Serum B12 was found to be higher in male than in female workers and serum folic acid level were significantly higher in female than in male workers. Vitamin B12 deficiency was found in 2.3 per cent and folic acid deficiency in 6.9 per cent of the male workers. Serum vitamin B12 and folic acid levels were normal for female workers. The adequate serum levels of vitamin B12 and folic acid might be the result of the habit of the workers to consume tonic drinks which contain glucose, caffeine, and vitamins especially vitamins B6, and B12.

  20. [Construction and fermentation control of reductive TCA pathway for malic acid production in Saccharomyces cerevisiae].

    Science.gov (United States)

    Yan, Daojiang; Wang, Caixia; Zhou, Jiemin; Liu, Yilan; Yang, Maohua; Xing, Jianmin

    2013-10-01

    Malic acid is widely used in food, and chemical industries. Through overexpressing pyruvate carboxylase and malate dehydrogenase in pdc1-deficient Saccharomyces cerevisiae, malic acid was successfully produced through the reductive TCA pathway. No malic acid was detected in wild type Saccharomyces cerevisiae, however, 45 mmol/L malic acid was produced in engineered strain, and the concentration of byproduct ethanol also reduced by 18%. The production of malic acid enhanced 6% by increasing the concentration of Ca2+. In addition, the final concentration reached 52.5 mmol/L malic acid by addition of biotin. The increasing is almost 16% higher than that of the original strain.

  1. Transferability of different classical force fields for right and left handed α-helices constructed from enantiomeric amino acids.

    Science.gov (United States)

    Biswas, Santu; Sarkar, Sujit; Pandey, Prithvi Raj; Roy, Sudip

    2016-02-21

    Amino acids can form d and l enantiomers, of which the l enantiomer is abundant in nature. The naturally occurring l enantiomer has a greater preference for a right handed helical conformation, and the d enantiomer for a left handed helical conformation. The other conformations, that is, left handed helical conformations of the l enantiomers and right handed helical conformations of the d enantiomers, are not common. The energetic differences between left and right handed alpha helical peptide chains constructed from enantiomeric amino acids are investigated using quantum chemical calculations (using the M06/6-311g(d,p) level of theory). Further, the performances of commonly used biomolecular force fields (OPLS/AA, CHARMM27/CMAP and AMBER) to represent the different helical conformations (left and right handed) constructed from enantiomeric (D and L) amino acids are evaluated. 5- and 10-mer chains from d and l enantiomers of alanine, leucine, lysine, and glutamic acid, in right and left handed helical conformations, are considered in the study. Thus, in total, 32 α-helical polypeptides (4 amino acids × 4 conformations of 5-mer and 10-mer) are studied. Conclusions, with regards to the performance of the force fields, are derived keeping the quantum optimized geometry as the benchmark, and on the basis of phi and psi angle calculations, hydrogen bond analysis, and different long range helical order parameters.

  2. Water quality, fate of metals and predictive model validation of a constructed wetland treating acid mine drainage

    Energy Technology Data Exchange (ETDEWEB)

    Mitsch, W.J.; Wise, K.M. [Ohio State University, Columbus, OH (United States). School of Natural Resources

    1998-06-01

    The paper describes how 0.39 ha constructed wetland designed with 9 cells, including two anaerobic cells that were to stimulate dissimilatory sulfate reduction, was evaluated for its effect on water quality of a low-order acid mine drainage (AMD) stream in southeastern Ohio, USA. Emphasis was on the uptake and fate of selected metals and the accuracy of a simulation model that predicted this specific wetland`s behavior before it was built.

  3. A new modular indole synthesis. Construction of the highly strained CDEF parent tetracycle of nodulisporic acids A and B.

    Science.gov (United States)

    Smith, Amos B; Kürti, Laszló; Davulcu, Akin H

    2006-05-11

    [reaction: see text] Construction of the highly strained CDEF parent tetracycle, a structural motif found only in the potent ectoparasiticidal agents (+)-nodulisporic acids A and B and related congeners, has been achieved via a new modular indole synthesis, exploiting a sequential Stille cross-coupling/Buchwald-Hartwig union/cyclization tactic. The new indole synthesis holds the promise of rapid assembly of diverse, highly substituted indoles possessing uncommon substitution patterns.

  4. Cloning of a yeast gene coding for the glutamate synthase small subunit (GUS2) by complementation of Saccharomyces cerevisiae and Escherichia coli glutamate auxotrophs.

    Science.gov (United States)

    González, A; Membrillo-Hernández, J; Olivera, H; Aranda, C; Macino, G; Ballario, P

    1992-02-01

    A Saccharomyces cerevisiae glutamate auxotroph, lacking NADP-glutamate dehydrogenase (NADP-GDH) and glutamate synthase (GOGAT) activities, was complemented with a yeast genomic library. Clones were obtained which still lacked NADP-GDH but showed GOGAT activity. Northern analysis revealed that the DNA fragment present in the complementing plasmids coded for a 1.5kb mRNA. Since the only GOGAT enzyme so far purified from S. cerevisiae is made up of a small and a large subunit, the size of the mRNA suggested that the cloned DNA fragment could code for the GOGAT small subunit. Plasmids were purified and used to transform Escherichia coli glutamate auxotrophs. Transformants were only recovered when the recipient strain was an E. coli GDH-less mutant lacking the small GOGAT subunit. These data show that we have cloned the structural gene coding for the yeast small subunit (GUS2). Evidence is also presented indicating that the GOGAT enzyme which is synthesized in the E. coli transformants is a hybrid comprising the large E. coli subunit and the small S. cerevisiae subunit.

  5. Performance of the auxotrophic Saccharomyces cerevisiae BY4741 as host for the production of IL-1β in aerated fed-batch reactor: role of ACA supplementation, strain viability, and maintenance energy

    Directory of Open Access Journals (Sweden)

    Zueco Jesus

    2009-12-01

    Full Text Available Abstract Background Saccharomyces cerevisiae BY4741 is an auxotrophic commonly used strain. In this work it has been used as host for the expression and secretion of human interleukin-1β (IL1β, using the cell wall protein Pir4 as fusion partner. To achieve high cell density and, consequently, high product yield, BY4741 [PIR4-IL1β] was cultured in an aerated fed-batch reactor, using a defined mineral medium supplemented with casamino acids as ACA (auxotrophy-complementing amino acid source. Also the S. cerevisiae mutant BY4741 Δyca1 [PIR4-IL1β], carrying the deletion of the YCA1 gene coding for a caspase-like protein involved in the apoptotic response, was cultured in aerated fed-batch reactor and compared to the parental strain, to test the effect of this mutation on strain robustness. Viability of the producer strains was examined during the runs and a mathematical model, which took into consideration the viable biomass present in the reactor and the glucose consumption for both growth and maintenance, was developed to describe and explain the time-course evolution of the process for both, the BY4741 parental and the BY4741 Δyca1 mutant strain. Results Our results show that the concentrations of ACA in the feeding solution, corresponding to those routinely used in the literature, are limiting for the growth of S. cerevisiae BY4741 [PIR4-IL1β] in fed-batch reactor. Even in the presence of a proper ACA supplementation, S. cerevisiae BY4741 [PIR4-IL1β] did not achieve a high cell density. The Δyca1 deletion did not have a beneficial effect on the overall performance of the strain, but it had a clear effect on its viability, which was not impaired during fed-batch operations, as shown by the kd value (0.0045 h-1, negligible if compared to that of the parental strain (0.028 h-1. However, independently of their robustness, both the parental and the Δyca1 mutant ceased to grow early during fed-batch runs, both strains using most of the

  6. Calculations and measurements of the deuterium tunneling frequency in the propiolic acid-formic acid dimer and description of a newly constructed Fourier transform microwave spectrometer.

    Science.gov (United States)

    Sun, Ming; Wang, Yimin; Carey, Spencer J; Mitchell, Erik G; Bowman, Joel; Kukolich, Stephen G

    2013-08-28

    The concerted proton tunneling frequency for the propiolic acid-formic acid dimer was calculated using a relaxed ab initio double-well potential in the imaginary-frequency mode of the saddle point, and new measurements were made for the deuterated propiolic acid-formic acid (ProOD-FAOD) isotopologue. It is important to have consistent calculated tunneling frequency values between normal and deuterated isotopologues since parameters can be readily adjusted to get good agreement with one isotopologue. High-resolution rotational spectra of deuterated (ProOD-FAOD) dimer were measured using a newly constructed Fourier Transform microwave spectrometer. The new spectrometer has mirror size: 30 cm in diameter with a radius of curvature of 59 cm and is equipped with multiple-FID data collection (5-10 FID's for each gas pulse). For the deuterated (ProOD-FAOD) isotopologue, 45 rotational lines (a type: 34; b type: 11) were measured in the lowest tunneling states range between 6.5 GHz and 15.5 GHz. With the new high-resolution measurements of the tunneling doublets (b-dipole transitions), the double potential well responsible for the deuterium tunneling was depicted much more precisely. The two tunneling states are separated by 3.48 MHz. The rotational constants obtained in this work are quite helpful for further structure analysis as well.

  7. Novel (Phenylethynyl)pyrene-LNA Constructs for Fluorescence SNP Sensing in Polymorphic Nucleic Acid Targets

    DEFF Research Database (Denmark)

    Astakhova, Irina Kira; Samokhina, Evgeniya; Babu, B Ravindra;

    2012-01-01

    We describe fluorescent oligonucleotide probes labeled with novel (phenylethynyl)pyrene dyes attached to locked nucleic acids. Furthermore, we prove the utility of these probes for the effective detection of single-nucleotide polymorphisms in natural nucleic acids. High-affinity hybridization...... of the probes and excellent fluorescence responses to single-base mismatches in DNA/RNA targets are demonstrated in model dual-probe and doubly labeled probe formats. This stimulated us to develop two diagnostic systems for the homogeneous detection of a drug-resistance-causing mutation in HIV-1 protease c...

  8. Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast

    OpenAIRE

    Xiaoying Sun; Go Hirai; Masashi Ueki; Hiroshi Hirota; Qianqian Wang; Yayoi Hongo; Takemichi Nakamura; Yuki Hitora; Hidekazu Takahashi; Mikiko Sodeoka; Hiroyuki Osada; Makiko Hamamoto; Minoru Yoshida; Yoko Yashiroda

    2016-01-01

    Uptake of poor nitrogen sources such as branched-chain amino acids is repressed in the presence of high-quality nitrogen sources such as NH4 + and glutamate (Glu), which is called nitrogen catabolite repression. Amino acid auxotrophic mutants of the fission yeast Schizosaccharomyces pombe were unable to grow on minimal medium containing NH4Cl or Glu even when adequate amounts of required amino acids were supplied. However, growth of these mutant cells was recovered in the vicinity of colonies...

  9. CONSTRUCTION OF MODULAR FIELD-BIOREACTOR FOR ACID MINE DRAINAGE TREATMENT

    Science.gov (United States)

    The paper focuses on the improvements to engineered features of a passive technology that has been used for remediation of acid rock drainage (ARD). This passive remedial technology, a sulfate-reducing bacteria (SRB) bioreactor, takes advantage of the ability of SRB that, if sup...

  10. Synthesis, structures and properties of two new chiral rare earth-organic frameworks constructed by L/D-tartaric acid

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Gonghao; Zhang, Haitao; Miao, Hao; Wang, Jiahong [College of Chemistry and Chemical Engineering, State Key Laboratory of Materials-oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009 (China); Xu, Yan, E-mail: yanxu@njtech.edu.cn [College of Chemistry and Chemical Engineering, State Key Laboratory of Materials-oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009 (China); State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093 (China)

    2015-09-15

    Hydrothermal reactions of rare earth cerium with L- or D- tartaric acid afford a pair of novel chiral enantiomer coordination polymers, namely, [Ce(L-tart)(CH{sub 2}OHCH{sub 2}OH)(H{sub 2}O)]Cl (L-1) and [Ce(D-tart)(CH{sub 2}OHCH{sub 2}OH)(H{sub 2}O)]Cl (D-1). Their structures were determined by single crystal X-ray diffraction analyses and further characterized by elemental analyses, XRD, IR spectra, and TG analyses. The circular dichroism (CD) spectra and second-harmonic generation (SHG) efficiency measurements proved that they are of structural chirality in the bulk samples. To the best of our knowledge, the enantiomers of L-1 and D-1 are the first 2D chiral dilayer frameworks constructed from L/D-tartrate ligands, ancillary ligand ethanediol and lanthanide ion Ce. - Graphical abstract: Hydrothermal reactions of rare earth cerium with L- or D- tartaric acid afford a pair of novel chiral enantiomer coordination polymers, namely, [Ce(L-tart)(CH{sub 2}OHCH{sub 2}OH)(H{sub 2}O)]Cl (L-1) and [Ce(D-tart)(CH{sub 2}OHCH{sub 2}OH)(H{sub 2}O)]Cl (D-1). Structural analysis indicates that the enantiomers of L-1 and D-1 are the first 2D chiral dilayer frameworks constructed from L/D-tartrate ligands and ancillary ligands ethanediol reacted with lanthanide ions Ce.

  11. Research on the Construction of Tissue Engineering Scaffolds by Alginate-hyaluronic Acid with Different Characteristics

    Institute of Scientific and Technical Information of China (English)

    LI Qin-hua; LIU Li; LIN Dong-qing

    2014-01-01

    This research uses alginate and hyaluronic acid as the main component to prepare support, then explores the possibilities as a tissue engineering scaffold. Firstly, prepare HA with various average molecular weight and alginate with different viscosity, mix them up at a certain proportion and make it into a AlgCa 2+-HA composite scaffold with a film-forming method. This article discusses the feasibility of this scaffold used in tissue engineering field according to the consequence of moisture content testing, mechanical analysis, and scanning electron microscopy analysis. The structure and properties of AlgCa2+-HA composite scaffold are closely related to some factors such as average molecular weight of hyaluronic acid, hyaluronic acid concentration, alginate viscosity, cross-linking agents and processing technology. The AlgCa 2+-HA composite material, which is at different proportions and adding different cross-linking agent,has some certain characteristics:moisture content ranging from 50%to 95%, tensile strength between 2.69 N/mm2 and 4.299 N/mm2, and elongation at break is about 58%to 160%. The prepared AlgCa2+-HA composite scaffolds can be used as tissue engineering scaffolds resulting from its high moisture content, good mechanical properties and ideal pore structure.

  12. Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis.

    Science.gov (United States)

    Koo, Byoung-Mo; Kritikos, George; Farelli, Jeremiah D; Todor, Horia; Tong, Kenneth; Kimsey, Harvey; Wapinski, Ilan; Galardini, Marco; Cabal, Angelo; Peters, Jason M; Hachmann, Anna-Barbara; Rudner, David Z; Allen, Karen N; Typas, Athanasios; Gross, Carol A

    2017-03-22

    A systems-level understanding of Gram-positive bacteria is important from both an environmental and health perspective and is most easily obtained when high-quality, validated genomic resources are available. To this end, we constructed two ordered, barcoded, erythromycin-resistance- and kanamycin-resistance-marked single-gene deletion libraries of the Gram-positive model organism, Bacillus subtilis. The libraries comprise 3,968 and 3,970 genes, respectively, and overlap in all but four genes. Using these libraries, we update the set of essential genes known for this organism, provide a comprehensive compendium of B. subtilis auxotrophic genes, and identify genes required for utilizing specific carbon and nitrogen sources, as well as those required for growth at low temperature. We report the identification of enzymes catalyzing several missing steps in amino acid biosynthesis. Finally, we describe a suite of high-throughput phenotyping methodologies and apply them to provide a genome-wide analysis of competence and sporulation. Altogether, we provide versatile resources for studying gene function and pathway and network architecture in Gram-positive bacteria.

  13. Hydrogen-bonded Supramolecular Rosette Ribbon Constructed from Melamine with Isophthalic Acid

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiu-Lian

    2008-01-01

    The new complex of melamine (MA) with isophthalie acid (H2IA), [(HMA+)-(HIA-)]·2H2O, has been prepared and its structure was characterized by X-ray crystallography.The crystal is of triclinic, space group P1 with a = 7.0228(6), b = 9.1706(8), c = 12.170(1) A, α =95.337(2), β = 105.247(2), γ = 97.813(2)°, V= 742.3(1) A3, Mr= 328.30, Z= 2,Dc = 1.469 g/cm3,λ = 0.71073 A, μ(MoKa) = 0.121 mm-1 and F(000) = 344. The structure was refined to R =0.0389 and wR = 0.1093 for 2513 observed reflections with Ⅰ 2σ(Ⅰ). Melamine dimer and isophthalic acid anion are interlinked by N-H…N and N-H…O hydrogen bonds, resulting in a 1D rosette ribbon in which water molecules and amino groups are self-assembled into quasi-hexagonal patterns.

  14. Copper coordination polymers constructed from thiazole-5-carboxylic acid: Synthesis, crystal structures, and structural transformation

    Science.gov (United States)

    Meundaeng, Natthaya; Rujiwatra, Apinpus; Prior, Timothy J.

    2017-01-01

    We have successfully prepared crystals of thiazole-5-carboxylic acid (5-Htza) (L) and three new thiazole-5-carboxylate-based Cu2+ coordination polymers with different dimensionality, namely, 1D [Cu2(5-tza)2(1,10-phenanthroline)2(NO3)2] (1), 2D [Cu(5-tza)2(MeOH)2] (2), and 3D [Cu(5-tza)2]·H2O (3). These have been characterized by single crystal X-ray diffraction and thermogravimetry. Interestingly, the 2D network structure of 2 can directly transform into the 3D framework of 3 upon removal of methanol molecules at room temperature. 2 can also undergo structural transformation to produce the same 2D network present in the known [Cu(5-tza)2]·1.5H2O upon heat treatment for 2 h. This 2D network can adsorb water and convert to 3 upon exposure to air.

  15. A novel nanofiber Cur-loaded polylactic acid constructed by electrospinning

    Science.gov (United States)

    Thu Trang Mai, Thi; Thu Thuy Nguyen, Thi; Duong Le, Quang; Ngoan Nguyen, Thi; Cham Ba, Thi; Binh Nguyen, Hai; Bich Hoa Phan, Thi; Tran, Dai Lam; Phuc Nguyen, Xuan; Park, Jun Seo

    2012-06-01

    Curcumin (Cur), extracted from the Curcuma longa L. plant, is well known for its anti-tumor, anti-oxidant, anti-inflammatory and anti-bacterial properties. Nanofiber mats of polylactic acid (PLA) loading Cur (5 wt%) were fabricated by electrospinning (e-spinning). Morphology and structure of the fibers were characterized by field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared (FTIR) spectroscopy, respectively. The diameters of the obtained fibers varied from 200 to 300 nm. The release capacity of curcumin from curcumin-loaded PLA fibers was investigated in phosphate buffer saline (PBS) containing ethanol. After 24 h, 50% of the curcumin was released from curcumin-loaded PLA fibers. These results of electrospun (e-spun) fibers exhibit the potential for biomedical application.

  16. Microbial populations identified by fluorescence in situ hybridization in a constructed wetland treating acid coal mine drainage

    Energy Technology Data Exchange (ETDEWEB)

    Nicomrat, D.; Dick, W.A.; Tuovinen, O.H. [Ohio State University, Wooster, OH (United States). Environmental Science Graduate Programme

    2006-07-15

    Microorganisms are an integral part of the biogeochemical processes in wetlands, yet microbial communities in sediments within constructed wetlands receiving acid mine drainage (AMD) are only poorly understood. The purpose of this study was to characterize the microbial diversity and abundance in a wetland receiving AMD using fluorescence in situ hybridization (FISH) analysis. Seasonal samples of oxic surface sediments, comprised of Fe(III) precipitates, were collected from two treatment cells of the constructed wetland system. The pH of the bulk samples ranged between pH 2.1 and 3.9. Viable counts of acidophilic Fe and S oxidizers and heterotrophs were determined with a most probable number (MPN) method. The MPN counts were only a fraction of the corresponding FISH counts. The sediment samples contained microorganisms in the Bacteria (including the subgroups of acidophilic Fe- and S-oxidizing bacteria and Acidiphilium spp.) and Eukarya domains. Archaea were present in the sediment surface samples at < 0.01% of the total microbial community. The most numerous bacterial species in this wetland system was Acidithiobacillus ferrooxidans, comprising up to 37% of the bacterial population. Acidithiobacillus thiooxidans was also abundant.

  17. Enhancement of azo dye Acid Orange 7 removal in newly developed horizontal subsurface-flow constructed wetland.

    Science.gov (United States)

    Tee, Heng-Chong; Lim, Poh-Eng; Seng, Chye-Eng; Mohd Nawi, Mohd Asri; Adnan, Rohana

    2015-01-01

    Horizontal subsurface-flow (HSF) constructed wetland incorporating baffles was developed to facilitate upflow and downflow conditions so that the treatment of pollutants could be achieved under multiple aerobic, anoxic and anaerobic conditions sequentially in the same wetland bed. The performances of the baffled and conventional HSF constructed wetlands, planted and unplanted, in the removal of azo dye Acid Orange 7 (AO7) were compared at the hydraulic retention times (HRT) of 5, 3 and 2 days when treating domestic wastewater spiked with AO7 concentration of 300 mg/L. The planted baffled unit was found to achieve 100%, 83% and 69% AO7 removal against 73%, 46% and 30% for the conventional unit at HRT of 5, 3 and 2 days, respectively. Longer flow path provided by baffled wetland units allowed more contact of the wastewater with the rhizomes, microbes and micro-aerobic zones resulting in relatively higher oxidation reduction potential (ORP) and enhanced performance as kinetic studies revealed faster AO7 biodegradation rate under aerobic condition. In addition, complete mineralization of AO7 was achieved in planted baffled wetland unit due to the availability of a combination of aerobic, anoxic and anaerobic conditions.

  18. Construction of Yeast Vectors with Resistance to Geneticin

    Institute of Scientific and Technical Information of China (English)

    林会兰; 张广; 周全; 陈国强

    2002-01-01

    Two Escherichia coli-Saccharomyces cerevisiae shuttle vectors containing a resistance marker to geneticin (G418) are constructed. Both vectors contain a kanamycin-resistant marker (KanMX4) module coding aminoglycoside 3'-phosphotransferase (APH) that renders E. coli resistant to kanamycin and S. cerevisiae to geneticin. These vectors overcome the shortage of the conventional yeast vectors bearing HIS3, TRP1, LEU2, and URA3 modules as selection markers, which require hosts to be auxotrophic. Green fluorescent protein (GFP) is used as the reporter to examine the functions of the vectors. The vectors are powerful tools for the convenient cloning and controlled expression of genes in most S. cerevisiae strains.

  19. Novel lanthanide complexes constructed from 3, 4-dimethoxybenzoic acid: crystal structures, spectrum and thermochemical properties

    Energy Technology Data Exchange (ETDEWEB)

    Qi, Xiao-Xia; Wu, Jun-Chen [Testing and Analysis Center, Hebei Normal University, Shijiazhuang 050024 (China); College of Chemistry & Material Science, Hebei Normal University, Shijiazhuang 050024 (China); Ren, Ning, E-mail: ningren9@163.com [College of Chemical engineering & Material, Handan College, Handan 056005 (China); Zhao, Chun-Li [Raoyang High School of Hebei, Raoyang 053900 (China); Zhang, Jian-Jun, E-mail: jjzhang6@126.com [Testing and Analysis Center, Hebei Normal University, Shijiazhuang 050024 (China); College of Chemistry & Material Science, Hebei Normal University, Shijiazhuang 050024 (China); Zong, Guang-Cai; Gao, Jie [Testing and Analysis Center, Hebei Normal University, Shijiazhuang 050024 (China); College of Chemistry & Material Science, Hebei Normal University, Shijiazhuang 050024 (China)

    2015-09-10

    Graphical abstract: Four novel lanthanide coordination polymers [Ln(3,4-DMOBA){sub 3}(H{sub 2}O){sub 2}]·H{sub 2}O·C{sub 2}H{sub 5}OH (Ln = Sm(1), Tb(2), Dy(3), Ho(4); 3,4-DMOBA = 3, 4-dimethoxybenzoate) have been hydrothermal synthesized and characterized. Single crystal structures indicates 1 D stucture of the title complexes are linked by hydrogen bonds to form a three-dimensional (3-D) structure. Complex 2 exhibits the characteristic emission of Tb{sup 3+} ion({sup 5}D{sub 4} → {sup 7}F{sub 6-3}). The investigation of TG-FTIR and IR spectra of the evolved gases shows uncoordinated water and ethanol molecules tend to lose firstly, and then 3,4-DMOBA ligands begin to decompose. - Highlights: • Four lanthanide coordination polymers were synthesized and characterized. • 1-D chain structures of the title complexes are are linked by hydrogen bonds to form a 3-D structures. • Thermal decomposition processes of the title complexes were studied using TG-FTIR techniques. • IR spectra of evolved gases in thermal decomposition process were obtained and analyzed. - Abstract: Four novel lanthanide complexes [Ln(3,4-DMOBA){sub 3}(H{sub 2}O){sub 2}]·H{sub 2}O·C{sub 2}H{sub 5}OH (Ln = Sm(1), Tb(2), Dy(3), Ho(4); 3,4-DMOBA = 3, 4-dimethoxybenzoate) have been hydrothermal synthesized and characterized. Structural analyses reveal that adjacent lanthanide ions are connected by 3,4-DMOBA ligands adopting bridging bidentate mode to generate one-dimensional (1-D) structure with the uncoordinated water and ethanol molecules. 1-D structures are linked by hydrogen bonds to form a three-dimensional (3-D) structure, which is rarely observed in lanthanide carboxylic acids complexes. Under the radiation of UV light, complex 2 exhibits the characteristic emission of Tb{sup 3+} ion ({sup 5}D{sub 4} → {sup 7}F{sub 6-3}). The thermal decomposition mechanism is investigated by TG-FTIR technology. IR spectra of the evolved gases show that the uncoordinated water and ethanol

  20. Reversions of two proline-requiring auxotrophs of Haemophilus influenzae by N-methyl-N'-nitro-N-nitrosoguanidine and hydrazine

    Energy Technology Data Exchange (ETDEWEB)

    Kimball, R.F.

    1976-01-01

    New mutation detection systems are described for Haemophilus influenzae. They involve two independently isolated proline auxotrophs which appear to be mutants at different sites in a proline locus (proB) that is very closely linked to a locus (thd) for thymidine requirement. One of the mutants, proB1, appears to revert to prototrophy only by mutations at the locus. The other, proB2, reverts both by mutation at the locus and by unlinked suppressors. The latter account for about 90 percent of the reversions induced by MNNG and by HZ. The close linkage of proB to thd was used to distinguish between true revertants and suppressors by a transformation test. A comparison was made between the mutation induction kinetics of the different classes of revertants and mutations to novobiocin resistance with MNNG and HZ. The very different induction kinetics for these two mutagens previously reported for the novobiocin resistance system were also found for the proline systems. There were some differences between the detection systems, however, in the frequency of induced mutation relative to the spontaneous frequency and, in one case, in the form of the induction curve. It is concluded that the major features of the induction curves reflect the amount of damage done to DNA and so are general for all systems, but that there are some features which are locus- or site-specific.

  1. Construction of efficient Streptococcus zooepidemicus strains for hyaluoronic acid production based on identification of key genes involved in sucrose metabolism.

    Science.gov (United States)

    Zhang, Xuzhen; Wang, Man; Li, Tuanjie; Fu, Lixia; Cao, Wei; Liu, Hao

    2016-12-01

    Biosynthesis of polysaccharide hyaluoronic acid (HA) by Streptococcus zooepidemicus is a carbon-intensive process. The carbon flux and factor(s) restricting HA yield were not well understood. Here, we investigated the function of genes involved in sucrose metabolism and identified targets limiting HA yield, which were exploited to construct efficient S. zooepidemicus strains for HA production. The sucrose uptake was addressed by deletion of scrA and scrB, which encodes sucrose-PTS permease and sucrose-6-phosphate hydrolase, respectively. We found that scrB was essential for the growth of S. zooepidemicus and HA biosynthesis, and accumulation of sucrose-6-phosphate was toxic. ΔscrB could not grow in THY-sucrose medium, while ΔscrA and ΔscrAΔscrB showed negligible growth defects. Overexpression of scrA significantly reduced biomass and HA production, while overexpression of scrB resulted in 26% increase of biomass and 30% increase of HA yield. We revealed that fructose-6-phosphate for HA biosynthesis mainly originates from glucose-6-phosphate. Deletion of scrK, a gene encoding hexokinase, led to 11% reduction of biomass and 12% decrease of HA yield, while deletion of hasE, a gene encoding phosphoglucoisomerase, resulted in the abolishment of HA biosynthesis and a significantly slow growth. We found that HA biosynthesis could be improved by directing carbon flux to fructose-6-phosphate. Deletion of fruA encoding the EII of fructose-PTS and fruK encoding phosphofructokinase showed no apparent effect on cell growth, but resulted in 22 and 27% increase of HA yield, respectively. Finally, a strain with 55% increase of HA was constructed by overexpression of scrB in ΔfruK. These results provide a solid foundation for further metabolic engineering of S. zooepidemicus for highly efficient HA production.

  2. Construction and characterization of pta gene-deleted mutant of Clostridium tyrobutyricum for enhanced butyric acid fermentation.

    Science.gov (United States)

    Zhu, Ying; Liu, Xiaoguang; Yang, Shang-Tian

    2005-04-20

    Clostridium tyrobutyricum ATCC 25755 is an acidogenic bacterium, producing butyrate and acetate as its main fermentation products. In order to decrease acetate and increase butyrate production, integrational mutagenesis was used to disrupt the gene associated with the acetate formation pathway in C. tyrobutyricum. A nonreplicative integrational plasmid containing the phosphotransacetylase gene (pta) fragment cloned from C. tyrobutyricum by using degenerate primers and an erythromycin resistance cassette were constructed and introduced into C. tyrobutyricum by electroporation. Integration of the plasmid into the homologous region on the chromosome inactivated the target pta gene and produced the pta-deleted mutant (PTA-Em), which was confirmed by Southern hybridization. SDS-PAGE and two-dimensional protein electrophoresis results indicated that protein expression was changed in the mutant. Enzyme activity assays using the cell lysate showed that the activities of PTA and acetate kinase (AK) in the mutant were reduced by more than 60% for PTA and 80% for AK. The mutant grew more slowly in batch fermentation with glucose as the substrate but produced 15% more butyrate and 14% less acetate as compared to the wild-type strain. Its butyrate productivity was approximately 2-fold higher than the wild-type strain. Moreover, the mutant showed much higher tolerance to butyrate inhibition, and the final butyrate concentration was improved by 68%. However, inactivation of pta gene did not completely eliminate acetate production in the fermentation, suggesting the existence of other enzymes (or pathways) also leading to acetate formation. This is the first-reported genetic engineering study demonstrating the feasibility of using a gene-inactivation technique to manipulate the acetic acid formation pathway in C. tyrobutyricum in order to improve butyric acid production from glucose.

  3. Construction and characterization of ack deleted mutant of Clostridium tyrobutyricum for enhanced butyric acid and hydrogen production.

    Science.gov (United States)

    Liu, Xiaoguang; Zhu, Ying; Yang, Shang-Tian

    2006-01-01

    Clostridium tyrobutyricum produces butyrate, acetate, H(2), and CO(2) as its main fermentation products from glucose and xylose. To improve butyric acid and hydrogen production, integrational mutagenesis was used to create a metabolically engineered mutant with inactivated ack gene, encoding acetate kinase (AK) associated with the acetate formation pathway. A non-replicative plasmid containing the acetate kinase gene (ack) fragment was constructed and introduced into C. tyrobutyricum by electroporation. Integration of the plasmid into the homologous region on the chromosome should inactivate the target ack gene and produce ack-deleted mutant, PAK-Em. Enzyme activity assays showed that the AK activity in PAK-Em decreased by approximately 50%; meanwhile, phosphotransacetylase (PTA) and hydrogenase activities each increased by approximately 40%. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results showed that the expression of protein with approximately 32 kDa molecular mass was reduced significantly in the mutant. Compared to the wild type, the mutant grew more slowly at pH 6.0 and 37 degrees C, with a lower specific growth rate of 0.14 h(-1) (vs 0.21 h(-1) for the wild type), likely due to the partially impaired PTA-AK pathway. However, the mutant produced 23.5% more butyrate (0.42 vs 0.34 g/g glucose) at a higher final concentration of 41.7 g/L (vs 19.98 g/L) as a result of its higher butyrate tolerance as indicated in the growth kinetics study using various intial concentrations of butyrate in the media. The mutant also produced 50% more hydrogen (0.024 g/g) from glucose than the wild type. Immobilized-cell fermentation of PAK-Em in a fibrous-bed bioreactor (FBB) further increased the final butyric acid concentration (50.1 g/L) and the butyrate yield (0.45 g/g glucose). Furthermore, in the FBB fermentation at pH 5.0 with xylose as the substrate, only butyric acid was produced by the mutant, whereas the wild type produced large amounts

  4. Construction of transplastomic lettuce (Lactuca sativa) dominantly producing astaxanthin fatty acid esters and detailed chemical analysis of generated carotenoids.

    Science.gov (United States)

    Harada, Hisashi; Maoka, Takashi; Osawa, Ayako; Hattan, Jun-Ichiro; Kanamoto, Hirosuke; Shindo, Kazutoshi; Otomatsu, Toshihiko; Misawa, Norihiko

    2014-04-01

    The plastid genome of lettuce (Lactuca sativa L.) cv. Berkeley was site-specifically modified with the addition of three transgenes, which encoded β,β-carotenoid 3,3'-hydroxylase (CrtZ) and β,β-carotenoid 4,4'-ketolase (4,4'-oxygenase; CrtW) from a marine bacterium Brevundimonas sp. strain SD212, and isopentenyl diphosphate isomerase from a marine bacterium Paracoccus sp. strain N81106. Constructed transplastomic lettuce plants were able to grow on soil at a growth rate similar to that of non-transformed lettuce cv. Berkeley and generate flowers and seeds. The germination ratio of the lettuce transformants (T0) (98.8%) was higher than that of non-transformed lettuce (93.1 %). The transplastomic lettuce (T1) leaves produced the astaxanthin fatty acid (myristate or palmitate) diester (49.2% of total carotenoids), astaxanthin monoester (18.2%), and the free forms of astaxanthin (10.0%) and the other ketocarotenoids (17.5%), which indicated that artificial ketocarotenoids corresponded to 94.9% of total carotenoids (230 μg/g fresh weight). Native carotenoids were there lactucaxanthin (3.8%) and lutein (1.3 %) only. This is the first report to structurally identify the astaxanthin esters biosynthesized in transgenic or transplastomic plants producing astaxanthin. The singlet oxygen-quenching activity of the total carotenoids extracted from the transplastomic leaves was similar to that of astaxanthin (mostly esterified) from the green algae Haematococcus pluvialis.

  5. Increased resveratrol production in wines using engineered wine strains Saccharomyces cerevisiae EC1118 and relaxed antibiotic or auxotrophic selection.

    Science.gov (United States)

    Sun, Ping; Liang, Jing-Long; Kang, Lin-Zhi; Huang, Xiao-Yan; Huang, Jia-Jun; Ye, Zhi-Wei; Guo, Li-Qiong; Lin, Jun-Fang

    2015-01-01

    Resveratrol is a polyphenolic compound with diverse beneficial effects on human health. Red wine is the major dietary source of resveratrol but the amount that people can obtain from wines is limited. To increase the resveratrol production in wines, two expression vectors carrying 4-coumarate: coenzyme A ligase gene (4CL) from Arabidopsis thaliana and resveratrol synthase gene (RS) from Vitis vinifera were transformed into industrial wine strain Saccharomyces cerevisiae EC1118. When cultured with 1 mM p-coumaric acid, the engineered strains grown with and without the addition of antibiotics produced 8.249 and 3.317 mg/L of trans-resveratrol in the culture broth, respectively. Resveratrol content of the wine fermented with engineered strains was twice higher than that of the control, indicating that our engineered strains could increase the production of resveratrol during wine fermentation.

  6. Construction and Verification of LuxS-negative Mutants of Streptococcus Mutans and the Effect of the Absence of LuxS Gene on the Acid Tolerance

    Institute of Scientific and Technical Information of China (English)

    YU Dan-ni; CHEN Jie; ZHANG Yao-chao; HAN Yu-zhi

    2009-01-01

    Objective: To knock out the entire Luxs gene of Streptococcus mutans(S.mutans) UA159 strain via homologous recombination and construct a Luxs-deleted mutant strain of S. Mutans. To study the difference between the acid resistance of S. Mutans Ingbritt C international standard strain and the acid resistance of LuxS mutant strain. Methods: Two DNA fragments locating in the upper and downstream of Luxs gene were amplified and a erythromycin resistance gene of PJT10 between them were engineered into PUC19 plasmid for constructing the recombination plasmid pUCluxKO. Electrotransformation of S.mutans cells with pUCluxKO-mutant resulted in isolation of erythromycin resistant S. Mutans transformants, which was identified by polymerase chain reaction, V.harveyi BB170 luminescence bioassay and sequencing analysis. Solutions of S. Mutans standard strain and LuxS mutant strain with same density were made and cultured at pH 3.5 to 7.0 BHI liquid for the same period.Terminal growth situation was compared.Firstly acidized in pH 5.5 BHI liquid,the two strains were cultured at pH 3.0 BHI liquid. The acid tolerance responses of the two strains were compared.Results:Restriction endonuclease analyses showed that pUCluxKO-mutant vector had been successfully recombined. The Luxs-deleted status of S.mutans mutants was confirmed by PCR with primers which were specific for the genes of Luxs and Erythromycin resistance. S.mutans mutant can not induce bioluminescence, indiating the mutant had been successfully recombined. After twenty generations of culture, the constructed Chinese S.mutans mutants were confirmed to be stable. Significant difference of aciduricity was observed between S.mutans standard strain and LuxS mutant strain.The acid resistance of standard strain was stronger than that of LuxS mutant strain.The two strains both displayed the capability of acid tolerance responses. Conclusion:The S.mutans gene allelic exchange plasmid is constructed correctively and a Luxs

  7. In vivo reshaping the catalytic site of nucleoside 2'-deoxyribosyltransferase for dideoxy- and didehydronucleosides via a single amino acid substitution.

    Science.gov (United States)

    Kaminski, Pierre Alexandre; Dacher, Priscilla; Dugué, Laurence; Pochet, Sylvie

    2008-07-18

    Nucleoside 2'-deoxyribosyltransferases catalyze the transfer of 2-deoxyribose between bases and have been widely used as biocatalysts to synthesize a variety of nucleoside analogs. The genes encoding nucleoside 2'-deoxyribosyltransferase (ndt) from Lactobacillus leichmannii and Lactobacillus fermentum underwent random mutagenesis to select variants specialized for the synthesis of 2',3'-dideoxynucleosides. An Escherichia coli strain, auxotrophic for uracil and unable to use 2',3'-dideoxyuridine, cytosine, and 2',3'-dideoxycytidine as a source of uracil was constructed. Randomly mutated lactobacilli ndt libraries from two species, L. leichmannii and L. fermentum, were screened for the production of uracil with 2',3'-dideoxyuridine as a source of uracil. Several mutants suitable for the synthesis of 2',3'-dideoxynucleosides were isolated. The nucleotide sequence of the corresponding genes revealed a single mutation (G --> A transition) leading to the substitution of a small aliphatic amino acid by a nucleophilic one, A15T (L. fermentum) or G9S (L. leichmannii), respectively. We concluded that the "adaptation" of the nucleoside 2'-deoxyribosyltransferase activity to 2,3-dideoxyribosyl transfer requires an additional hydroxyl group on a key amino acid side chain of the protein to overcome the absence of such a group in the corresponding substrate. The evolved proteins also display significantly improved nucleoside 2',3'-didehydro-2',3'-dideoxyribosyltransferase activity.

  8. Multicomponent hydrogen-bonding organic solids constructed from 6-hydroxy-2-naphthoic acid and N-heterocycles: Synthesis, structural characterization and synthon discussion

    Science.gov (United States)

    Zong, Yingxia; Shao, Hui; Pang, Yanyan; Wang, Debao; Liu, Kang; Wang, Lei

    2016-07-01

    Seven novel multicomponent crystals involving various substituted organic amine molecules and 6-hydroxy-2-naphthoic acid were prepared and characterized by using single crystal X-ray diffraction, infrared and thermogravimetric analyses (TGA). Crystal structures with 1,4-bis(imidazol) butane (L1) 1, 1,4-bis(imidazol-1-ylmethyl)benzene (L2) 2, 1-phenyl piperazine 3, 2-amino-4-hydroxy-6-methyl pyrimidine 4, 4,4'-bipyridine 5, 5,5'-dimethyl-2,2'-dipyridine 6, 2-amino-4,6-dimethyl pyrimidine 7 were determined. Among the seven molecular complexes, total proton transfer from 6-hydroxy-2-naphthoic acid to coformer has occurred in crystals 1-4, while the remaining were cocrystals. X-ray single-crystal structures of these complexes reveal that strong hydrogen bonding O-H···O/N-H···O/O-H···N and weak C-H···O/C-H···π/π···π intermolecular interactions direct the packing modes of molecular crystals together. The analysis of supramolecular synthons in the present structures shows that some classical supramolecular synthons like pyridine-carboxylic acid heterosynthon R22 (7) and aminopyridine-carboxylic acid heterosynthon R22 (8), are again observed in constructing the hydrogen-bonding networks in this paper. Besides, we noticed that water molecules act as a significant hydrogen-bonding connector in constructing supramolecular architectures of 3, 4, 6, and 7.

  9. 谷氨酸棒杆菌L-色氨酸营养缺陷型突变及其高产菌株的选育%Study on breeding of Corynebacterium glutamicum L-tryptophan auxotrophic strains with high yield

    Institute of Scientific and Technical Information of China (English)

    张新武; 侯钢北; 杨晓明; 廉娜娜

    2015-01-01

    Objective To research the breeding of high L-tryptophan yield with multiple synthetic and metabolic pathway mutation strain by using Corynebacterium glutamicum HX-22 as the starting strain. Methods Diethyl sulfate (DES), UV, and cobalt-60 gamma-ray mutagenesis method were performed cross processing starting on C. glutamicum HX-22 and mutant strain. The auxotrophic phenotype and metabolic suicide substrate resistance screening methods were used, and targeted mutagenesis and breeding of L-tryptophan acid synthesis and catabolism mutation of lubricious ammonia acid producing strain. Results Through continuous type defect mutagenesis breeding for many times, the strains with auxotroph and resistance marker of L-tryptophan high yield bacteria HX22-118 (Phe--Tyr--5FTr-4FPr-SGr) was screened, then extend the 10 times in succeeding transfer culture, the L-trp fermentation yield reached to 27.1 g/L, which was increased by 81.8% comparing to the wild start strain. Conclusion The selected multiple mutated strain HX22-118 (Phe--Tyr--5FTr-4FPr-SGr) with high L-trp yielding strain has a good genetic stability, which can be applied in industrial application.%目的:以谷氨酸棒杆菌(Corynebacterium glutamicum)HX-22为出发菌株,研究了目标发酵产物L-色氨酸合成途径交叉反馈抑制途径代谢突变与色氨酸分解代谢类似物抗性的营养缺陷型突变诱导过程及 L-色氨酸高产复合突变菌株的筛选。方法采用硫酸二乙酯、紫外线、钴60γ-射线等诱变方法交叉处理起始与突变菌株,通过营养缺陷表型和自杀代谢底物抗性的筛选方法,定向突变和选育 L-色氨酸合成与分解代谢突变的色氨酸高产菌株。结果通过多次连续营养缺陷型诱变选育,筛选出一株具有分支酸-Trp/Phe/Tyr代谢途径L-Phe和 L-Tyr 合成缺陷型和 L-Trp 分解代谢自杀代谢底物抗性的 L-色氨酸高产菌 HX22-118(Phe--Tyr--5FTr-4FPr-SGr);通过连续传代10次

  10. Construction of amperometric uric acid biosensor based on uricase immobilized on PBNPs/cMWCNT/PANI/Au composite.

    Science.gov (United States)

    Rawal, Rachna; Chawla, Sheetal; Chauhan, Nidhi; Dahiya, Tulika; Pundir, C S

    2012-01-01

    A chitosan-glutaraldehyde crosslinked uricase was immobilized onto Prussian blue nanoparticles (PBNPs) absorbed onto carboxylated multiwalled carbon nanotube (c-MWCNT) and polyaniline (PANI) layer, electrochemically deposited on the surface of Au electrode. The nanohybrid-uricase electrode was characterized by scanning electron microscopic (SEM), Fourier transform infrared spectroscopy (FTIR) and cyclic voltammetry. An amperometric uric acid biosensor was fabricated using uricase/c-MWCNT/PBNPs/Au electrode as working electrode, Ag/AgCl as standard and Pt wire as auxiliary electrode connected through a potentiostat. The biosensor showed optimum response within 4s at pH 7.5 and 40°C, when operated at 0.4V vs. Ag/AgCl. The linear working range for uric acid was 0.005-0.8 mM, with a detection limit of 5 μM. The sensor was evaluated with 96% recovery of added uric acid in sera and 4.6 and 5.4% within and between batch of coefficient of variation respectively and a good correlation (r=0.99) with standard enzymic colorimetric method. This sensor measured uric acid in real serum samples. The sensor lost only 37% of its initial activity after its 400 uses over a period of 7 months, when stored at 4°C.

  11. Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast.

    Science.gov (United States)

    Sun, Xiaoying; Hirai, Go; Ueki, Masashi; Hirota, Hiroshi; Wang, Qianqian; Hongo, Yayoi; Nakamura, Takemichi; Hitora, Yuki; Takahashi, Hidekazu; Sodeoka, Mikiko; Osada, Hiroyuki; Hamamoto, Makiko; Yoshida, Minoru; Yashiroda, Yoko

    2016-02-19

    Uptake of poor nitrogen sources such as branched-chain amino acids is repressed in the presence of high-quality nitrogen sources such as NH4(+) and glutamate (Glu), which is called nitrogen catabolite repression. Amino acid auxotrophic mutants of the fission yeast Schizosaccharomyces pombe were unable to grow on minimal medium containing NH4Cl or Glu even when adequate amounts of required amino acids were supplied. However, growth of these mutant cells was recovered in the vicinity of colonies of the prototrophic strain, suggesting that the prototrophic cells secrete some substances that can restore uptake of amino acids by an unknown mechanism. We identified the novel fatty acids, 10(R)-acetoxy-8(Z)-octadecenoic acid and 10(R)-hydroxy-8(Z)-octadecenoic acid, as secreted active substances, referred to as Nitrogen Signaling Factors (NSFs). Synthetic NSFs were also able to shift nitrogen source utilization from high-quality to poor nitrogen sources to allow adaptive growth of the fission yeast amino acid auxotrophic mutants in the presence of high-quality nitrogen sources. Finally, we demonstrated that the Agp3 amino acid transporter was involved in the adaptive growth. The data highlight a novel intra-species communication system for adaptation to environmental nutritional conditions in fission yeast.

  12. Comparative genomics of the lactic acid bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Makarova, K.; Slesarev, A.; Wolf, Y.; Sorokin, A.; Mirkin, B.; Koonin, E.; Pavlov, A.; Pavlova, N.; Karamychev, V.; Polouchine, N.; Shakhova, V.; Grigoriev, I.; Lou, Y.; Rokhsar, D.; Lucas, S.; Huang, K.; Goodstein, D. M.; Hawkins, T.; Plengvidhya, V.; Welker, D.; Hughes, J.; Goh, Y.; Benson, A.; Baldwin, K.; Lee, J. -H.; Diaz-Muniz, I.; Dosti, B.; Smeianov, V; Wechter, W.; Barabote, R.; Lorca, G.; Altermann, E.; Barrangou, R.; Ganesan, B.; Xie, Y.; Rawsthorne, H.; Tamir, D.; Parker, C.; Breidt, F.; Broadbent, J.; Hutkins, R.; O' Sullivan, D.; Steele, J.; Unlu, G.; Saier, M.; Klaenhammer, T.; Richardson, P.; Kozyavkin, S.; Weimer, B.; Mills, D.

    2006-06-01

    Lactic acid-producing bacteria are associated with various plant and animal niches and play a key role in the production of fermented foods and beverages. We report nine genome sequences representing the phylogenetic and functional diversity of these bacteria. The small genomes of lactic acid bacteria encode a broad repertoire of transporters for efficient carbon and nitrogen acquisition from the nutritionally rich environments they inhabit and reflect a limited range of biosynthetic capabilities that indicate both prototrophic and auxotrophic strains. Phylogenetic analyses, comparison of gene content across the group, and reconstruction of ancestral gene sets indicate a combination of extensive gene loss and key gene acquisitions via horizontal gene transfer during the coevolution of lactic acid bacteria with their habitats.

  13. Chemical mutagenesis of Gluconobacter frateurii to construct methanol-resistant mutants showing glyceric acid production from methanol-containing glycerol.

    Science.gov (United States)

    Sato, Shun; Kitamoto, Dai; Habe, Hiroshi

    2014-02-01

    To produce glyceric acid (GA) from methanol-containing glycerol, resistance to methanol of Gluconobacter frateurii NBRC103465 was improved by chemical mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine. The obtained mutant Gf398 produced 6.3 g/L GA in 5% (v/v) methanol-containing 17% (w/v) glycerol medium, in which the wild-type strain neither grew nor produced GA.

  14. Construction and Study of Hetreojunction Solar Cell Based on Dodecylbenzene Sulfonic Acid-Doped Polyaniline/n-Si

    OpenAIRE

    Morsi, I.; Sh. Ebrahim; Soliman, M

    2012-01-01

    Polyaniline/n-type Si heterojunctions solar cell are fabricated by spin coating of soluble dodecylbenzene sulfonic acid (DBSA)-doped polyaniline onto n-type Si substrate. The electrical characterization of the Al/n-type Si/polyaniline/Au (Ag) structure was investigated by using current-voltage (I-V), capacitance-voltage (C-V), and impedance spectroscopy under darkness and illumination. The photovoltaic cell parameters, that is, open-circuit voltage (oc), short-circuit current density (sc), ...

  15. Construction of composite electrodes comprising manganese dioxide nanoparticles distributed in polyaniline-poly(4-styrene sulfonic acid-co-maleic acid) for electrochemical supercapacitor

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Feng-Jiin; Hsu, Tsui-Fen [Department of Chemical Engineering, National United University, 1, Lien Da, Kung-Ching Li, Miao-Li 36003 (China); Yang, Chien-Hsin [Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 811 (China)

    2009-06-15

    This work demonstrated a novel and simple route for preparing a composite comprising of manganese oxide (MnO{sub 2}) nanoparticles and polyaniline (PANI) doped poly(4-styrene sulfonic acid-co-maleic acid) (PSSMA) by ''electrochemical doping-deposition''. The PANI-PSSMA-MnO{sub 2} composite was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). SEM images revealed a uniform dispersion of MnO{sub 2} nanoparticles in the porous structure of PANI-PSSMA structure. XRD measurements showed the distortion of the crystal structure of {beta}-MnO{sub 2} after deposition of MnO{sub 2} in PANI-PSSMA structure. Thus, the XRD pattern of PANI was predominating. Cyclic voltammetry and chronopotentiometry were employed in 0.5 M Na{sub 2}SO{sub 4} to evaluate the capacitor properties. The results showed a significant improvement in the specific capacitance of the composite electrode. The specific capacitance of PANI-PSSMA-MnO{sub 2} (50.4 F g{sup -1}) had improvement values of 172% compared to that of PANI (18.5 F g{sup -1}). When only the MnO{sub 2} mass was considered, the composite had a specific capacitance of 556 F g{sup -1}. (author)

  16. Synthesis and Crystal Structure of a Novel 1D Magnesium (Ⅱ) Coordination Polymer Constructed by L-Cysteic Acid

    Institute of Scientific and Technical Information of China (English)

    LI Hai-Ye; LIAO Bei-Ling; JIANG Yi-Min; ZHANG Shu-Hua; LI Jun-Xia

    2007-01-01

    A novel coordination polymer [Mg(L)(H2O)2]·H2O] (LH2=L-cysteic acid) has been synthesized and characterized by elemental analysis, IR and single-crystal X-ray diffraction.The crystal crystallizes in orthorhombic system, space group P212121, with a=5.962(3), b=11.224(6), c=13.664(7)(A), V=914.3(8) (A)3, Z=4, Mr=245.50, Dc=1.783 g/cm3,μ=0.445 mm-1,flack parameter=0.32(16), F(000)=512, the final R=0.0458 and wR=0.1172 for 1578 observed reflections with I > 2σ(Ⅰ). The Mg(Ⅱ) atom shows an octahedral geometry defined by two carboxyl O atoms from two different L-cysteic acid ligands, one carboxyl O atom and one amino N atom from the adjacent ligand, and two aqua ligands. The Mg(Ⅱ) atoms are bridged by L-cysteic acid ligands, leading to a 1D infinite zigzag chain. In the structure there are extensive hydrogen bonds,through which the complex completes its 3D framework structure.

  17. Combination of nucleic acid and protein isolation with tissue array construction: using defined histologic regions in single frozen tissue blocks for multiple research purposes.

    Science.gov (United States)

    Li, Hong; Sun, Yuan; Kong, Qing-You; Zhang, Kai-Li; Wang, Xiao-Wei; Chen, Xiao-Yan; Wang, Qian; Liu, Jia

    2003-09-01

    Precise dissection of defined histological regions for nucleic acid and protein isolation is a precedent step in finding out cancer-related alterations, and high quality tissue microarrays are demanded in the validation of screened genetic alterations by multiple in situ approaches. In this study, a combined technique was developed by which sample isolation and tissue array construction could be performed on the defined morphological region(s) in single tissue block. The RNA and protein samples generated from the selected portions were of good quality and sufficient for multiple experimental purposes. The frozen tissue arrays constructed on a novel recipient are suitable for multiple in situ evaluations including immunohistochemical staining and mRNA hybridisation. In most cases, the data obtained from in situ assays coincided well with the ones revealed by RT-PCR and Western blot hybridisation. The potential experimental bias caused by cell contamination can be amended by tissue array-based retrospective examination. The combination of tissue-selective sample preparations with tissue array construction thus provide a tool by which comprehensive cancer research can be performed on defined histological regions in a series of single frozen tissue blocks.

  18. Construction and expression of nucleic acid vaccine pVAXl-h-alpha S1-14o coding human alpha-synuclein

    Institute of Scientific and Technical Information of China (English)

    Jiacai Wang; Yingsong Ouyang; Shaojun Wang; Guoguang Peng; Qin Luo; Side Jiang; Faxiang Wang

    2008-01-01

    BACKGROUND: The deposition of α-synuclein (α-syn) aggregates is a neuropathological feature of Parkinson's disease. It remains impossible to involve α-syn aggregation in the treatment of Parkinson's disease. A nucleic acid vaccine will provide a new pathway to immunotherapy for Parkinson's disease.OBJECTIVE: To construct a recombinant eukaryotic expression vector pVAX1 coding human α-syn and to observe its expression level in COS-7 cells.DESIGN AND SETTING: The present bioengineering and molecular biology experiment was performed at Department of Neurology, First Affiliated Hospital of Chongqing Medical University & Chongqing Key Laboratory of Neurology.MATERIALS: The eukaryotic expression plasmid pVAX1, human embryonic brain tissue, healthy human blood cells, and COS-7 cells were purchased from Promega Company, USA.METHODS: The full-length CDS sequence of the human α-syn gene was amplified by RT-PCR, which contained restriction sites for the enzymes Kpn I, Xba I and Kozak consensus sequence. Then the PCR products and eukaryotic expression vector pVAX1 were digested with Kpn I and Xba I simultaneously, and were extracted and ligated by T4 ligase. The recombinant constructs pVAXI-hα-S1-14o were transformed into competent E. coli TOP 10 cells and the positive clones were screened and selected using PCR analysis,restriction digestion analysis, and DNA sequencing. The constructs were then tested for protein expression in COS-7 cells by RT-PCR and Western blotting.MAIN OUTCOME MEASURES: Identification of an eukaryotic expression vector containing the human α-syn gene, pVAX1-hα-S1-140, and detection of the expression in mammalian cell COS-7.RESULTS: The pVAXi vector was successfully cloned with human a -syn in the correct orientation and in-frame. The DNA vaccine constructs pVAX1-hα-S1-140 with the human α-syn gene were shown to be expressed in COS-7 cells. Human α-syn was successfully expressed in the mammalian cell line and was detected by RT-PCR and western

  19. The influence of ascorbic acid, TGF-beta1, and cell-mediated remodeling on the bulk mechanical properties of 3-D PEG-fibrinogen constructs.

    Science.gov (United States)

    Kim, Peter D; Peyton, Shelly R; VanStrien, Amy J; Putnam, Andrew J

    2009-08-01

    Two-dimensional cell culture studies have shown that matrix rigidity can influence cell function, but little is known about how matrix physical properties, and their changes with time, influence cell function in 3-D. Biosynthetic hydrogels based on PEGylated fibrinogen permit the initial decoupling of matrix chemical and mechanical properties, and are thus potentially attractive for addressing this question. However, the mechanical stability of these gels due to passive hydrolysis and cell-mediated remodeling has not previously been addressed. Here, we show that the bulk mechanical properties of acellular PEG-fibrinogen hydrogels significantly decrease over time in PBS regardless of matrix cross-linking density in 7 days. To compensate, smooth muscle cells (SMCs) were encapsulated and stimulated to produce their own matrix using ascorbic acid or TGF-beta1. Ascorbic acid treatment improved the mechanical properties of the constructs after 14 days in less cross-linked matrices, but TGF-beta1 did not. The increase in matrix modulus of the constructs was not due to an increase in type I collagen deposition, which remained low and pericellular regardless of cross-link density or the soluble factor applied. Instead, ascorbic acid, but not TGF-beta1, preferentially enhanced the contractile SMC phenotype in the less cross-linked gels. Inhibition of contractility reduced cell spreading and the expression of contractile markers, and eliminated any beneficial increase in matrix modulus induced by cell-generated contraction of the gels. Together, these data show that PEG-fibrinogen hydrogels are susceptible to both hydrolysis and proteolysis, and suggest that some soluble factors may stimulate matrix remodeling by modulating SMC phenotype instead of inducing ECM synthesis in a 3-D matrix.

  20. A pH-responsive drug nanovehicle constructed by reversible attachment of cholesterol to PEGylated poly(l-lysine) via catechol-boronic acid ester formation.

    Science.gov (United States)

    Yang, Bin; Lv, Yin; Zhu, Jing-Yi; Han, Yun-Tao; Jia, Hui-Zhen; Chen, Wei-Hai; Feng, Jun; Zhang, Xian-Zheng; Zhuo, Ren-Xi

    2014-08-01

    The present work reports the construction of a drug delivery nanovehicle via a pH-sensitive assembly strategy for improved cellular internalization and intracellular drug liberation. Through spontaneous formation of boronate linkage in physiological conditions, phenylboronic acid-modified cholesterol was able to attach onto catechol-pending methoxypoly(ethylene glycol)-block-poly(l-lysine). This comb-type polymer can self-organize into a micellar nanoconstruction that is able to effectively encapsulate poorly water-soluble agents. The blank micelles exhibited negligible in vitro cytotoxicity, yet doxorubicin (DOX)-loaded micelles could effectively induce cell death at a level comparable to free DOX. Owing to the acid-labile feature of the boronate linkage, a reduction in environmental pH from pH 7.4 to 5.0 could trigger the dissociation of the nanoconstruction, which in turn could accelerate the liberation of entrapped drugs. Importantly, the blockage of endosomal acidification in HeLa cells by NH4Cl treatment significantly decreased the nuclear uptake efficiency and cell-killing effect mediated by the DOX-loaded nanoassembly, suggesting that acid-triggered destruction of the nanoconstruction is of significant importance in enhanced drug efficacy. Moreover, confocal fluorescence microscopy and flow cytometry assay revealed the effective internalization of the nanoassemblies, and their cellular uptake exhibited a cholesterol dose-dependent profile, indicating the contribution of introduced cholesterol functionality to the transmembrane process of the nanoassembly.

  1. 产L 苹果酸重组大肠杆菌的构建%Construction of recombinant Escherichia coli for L-malic acid production

    Institute of Scientific and Technical Information of China (English)

    吴亚斌; 张梁; 石贵阳

    2014-01-01

    Based on the synthesis pathway of succinic acid in E�coli B0013-1050,the synthesize pathway of L-malic acid was constructed by using Red homologus recombination combined with Xer/dif recombinase system to delete the gene fumB,fumC and maeB from chromosome,and a recombinant strain E�coli 2030 was obtained�L-malic acid of 12�5 g/L with yield of 52�1% was achieved in a 15-L bioreactor. The main by-products, including pyruvic acid and succinic acid were analyzed�To increase the yield of L-malic acid, the malate dehydrogenase encoded gene mdh from Aspergillus flavus was integrantly expressed as the recombinant strain and a recombinant strain E�coli 2040 was obtained. The concentration and the yield of L-malic acid increased to 14 g/L and 60�3% in a 15-L bioreactor, respectively�%基于产琥珀酸重组大肠杆菌E�coli B00131050的琥珀酸合成途径,利用Red同源重组技术结合Xer/dif重组系统敲除富马酸酶基因fumB、fumC,苹果酸酶基因maeB,构建L 苹果酸合成途径,最终得到重组大肠杆菌E�coli 2030,该菌株在15 L发酵罐中,产L 苹果酸12�5 g/L,葡萄糖苹果酸转化率为52�1%,同时对发酵产物中主要杂酸丙酮酸和琥珀酸的生产原因进行了初步的探讨与分析。为进一步提高L 苹果酸的转化率,整合表达来源于黄曲霉的苹果酸脱氢酶基因,构建重组菌E�coli 2040,在15 L发酵罐中产L 苹果酸14 g/L,葡萄糖苹果酸转化率提高到60�3%。

  2. Construction of antibacterial poly(ethylene terephthalate) films via layer by layer assembly of chitosan and hyaluronic acid.

    Science.gov (United States)

    Del Hoyo-Gallego, Sara; Pérez-Álvarez, Leyre; Gómez-Galván, Flor; Lizundia, Erlantz; Kuritka, Ivo; Sedlarik, Vladimir; Laza, Jose Manuel; Vila-Vilela, Jose Luis

    2016-06-05

    Polyelectrolytic multilayers (PEMs) with enhanced antibacterial properties were built up onto commercial poly(ethylene terephthalate) (PET) films based on the layer by layer assembling of bacterial contact killing chitosan and bacterial repelling highly hydrated hyaluronic acid. The optimization of the aminolysis modification reaction of PET was carried out by the study of the mechanical properties and the surface characterization of the modified polymers. The layer by layer assembly was successfully monitored by TEM microscopy, surface zeta-potential, contact angle measurements and, after labeling with fluorescein isothiocyanate (FTIC) by absorption spectroscopy and confocal fluorescent microscopy. Beside, the stability of the PEMs was studied at physiological conditions in absence and in the presence of lysozyme and hyaluronidase enzymes. Antibacterial properties of the obtained PEMs against Escherichia coli were compared with original commercial PET.

  3. Construction and Analysis of Three-dimensional Graphic Model of Single-chain Fv Derived from an Anti-human Placental Acidic Isoferritin Monoclonal Antibody by Computer

    Institute of Scientific and Technical Information of China (English)

    ZHOU Chun; SHEN Guanxin; ZHU Huifen; YANG Jing; ZHANG Yue; FENG Jiannan; SHEN Beifen

    2000-01-01

    A three-dimensional (3D) graphic model of a single-chain Fv (scFv) which was derived from an anti-human placental acidic isoferritin (PAF) monoclonal antibody (Mab) was constructed by a homologous protein-predicting computer algorithm on Silicon graphic computer station.The structure, surface static electricity and hydrophobicity of scFv were investigated. Computer graphic modelling indicated that all regions of scFv including the linker, variable regions of the heavy (VH) and light (VL) chains were suitable. The VH region and the VL region were involved in composing the "hydrophobic pocket". The linker was drifted away VH and VL regions. The complementarity determining regions (CDRs) of VH and VL regions surrounded the "hydrophobic pocket". This study provides a theory basis for improving antibody affinity, investigating antibody structure and analyzing the functions of VH and VL regions in antibody activity.

  4. Synthesis,Electrocatalytic Properties and Crystal Structure of a New Organic—Inorganic Hybrid Constructed from Dodecamolybdophosphoric Acid and Benzotriazole

    Institute of Scientific and Technical Information of China (English)

    HANZheng-bo; WANGXiu-li; WANGLi; WANGEn-bo; LUANGuo-you; HUNing-hai; JIAHeng-qing

    2003-01-01

    A new compound,(C6H6N2)7(PMo12ⅥO40)(PMoⅤMo11ⅥO40)·2CH3CH2OH·5H2O,was synthcsized and characterized by means of elemental analyses,IR spectroscopy,1H NMR spectroscopy and single crystal X-ray diffraction.This is the first example of benzotriazole-Polyoxometalates species.The compound crystallized in a triclinic space group P1 with a=1.8378(4)nm,b=1.9078(4)nm,c=2.1037(4)nm,a=63.41(3)°,γ=68.38(3)°,V=5.803(2)nm3,Z=2,R1=0.0486,ωR2=0.1357.The X-ray crystallographic study showed that the crystal structure was constructed by electrostatic interactions and hy-drogen bonds between dodecamolybdophosphorate anions and protonated benzotriazole cations.The electrochemical bchavior and the reduction of nitrite and hydrogen peroxide clctrocatalyzed by the title compound were studied.

  5. Construction and Study of Hetreojunction Solar Cell Based on Dodecylbenzene Sulfonic Acid-Doped Polyaniline/n-Si

    Directory of Open Access Journals (Sweden)

    I. Morsi

    2012-01-01

    Full Text Available Polyaniline/n-type Si heterojunctions solar cell are fabricated by spin coating of soluble dodecylbenzene sulfonic acid (DBSA-doped polyaniline onto n-type Si substrate. The electrical characterization of the Al/n-type Si/polyaniline/Au (Ag structure was investigated by using current-voltage (I-V, capacitance-voltage (C-V, and impedance spectroscopy under darkness and illumination. The photovoltaic cell parameters, that is, open-circuit voltage (oc, short-circuit current density (sc, fill factor (FF, and energy conversion efficiency (η were calculated. The highest sc, oc, and efficiency of these heterojunctions obtained using PANI-DBSA as a window layer (wideband gap and Au as front contact are 1.8 mA/cm2, 0.436 V, and 0.13%, respectively. From Mott-Schottky plots, it was found that order of charge carrier concentrations is 3.5×1014 and 1.0×1015/cm3 for the heterojunctions using Au as front contact under darknessness and illumination, respectively. Impedance study of this type of solar cell showed that the shunt resistance and series resistance decreased under illumination.

  6. Tetraarylboronic acid resorcinarenes: Synthesis, molecular recognition, and templates for the construction of three-dimensional electronic organic materials

    Science.gov (United States)

    Lewis, Patrick Tyrone

    The resorcinarenes are remarkable cyclic aromatic tetramers whose impressive impact in the disciplines of molecular recognition, materials science and supramolecular chemistry has been the subject of extensive study and recent review. The potential utility of these materials as chemosensors, catalysts, energy storage and drug delivery agents has been studied or proposed. Research involving resorcinarene molecular containers (careceplexes, carecerands, hemicarcerands) has resulted in landmark achievements including the stabilization of encapusulated cyclobutadiene and benzyne. Functionalization of the resorcinarene lower rim has begun to attract attention as a means to enhance the properties of the parent macrocycles. There has been only one report (1989), however, describing the extension of their lower cavity conjugation. We have performed direct, fourfold conjugation extension of the lower rim. Our findings would allow for the fabrication of heteropolyfunctional, directional molecular scaffolds embodying new receptors and supramolecular materials. We have thus (1) performed a gram scale synthesis and direct isolation of boronic acid functionalized stereoisomeric resorcinarenes; (2) polyfunctionalized at divergent macrocyclic sites, affording chiral and achiral resorcinarene octols and cavitands; (3) presented preliminary evidence that the relatively little-explored C2h resorcinarenes can compete effectively with their C4v counterparts in both covalent and non-covalent binding of polar guests; (4) reported their use in the colorimetric differentiation of carbohydrates; and (5) extended the lower cavity by fourfold aryl coupling under Suzuki conditions.

  7. Usefulness of biodegradable stents constructed of poly-/-lactic acid monofilaments in patients with benign esophageal stenosis

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    AIM: To report 13 patients with benign esophageal stenosis treated with the biodegradable stent.METHODS: We developed a Ultraflex-type stent by knitting poly-l-lactic acid monofilaments.RESULTS: Two cases were esophageal stenosis caused by drinking of caustic liquid, 4 cases were due to surgical resection of esophageal cancers, and 7 cases were patients with esophageal cancer who received the preventive placement of biodegradable stents for postendoscopic mucosal dissection (ESD) stenosis. The preventive placement was performed within 2 to 3 d after ESD. In 10 of the 13 cases, spontaneous migration of the stents occurred between 10 to 21 d after placement. In these cases, the migrated stents were excreted with the feces, and no obstructive complications were experienced. In 3 cases, the stents remained at the proper location on d 21 after placement. No symptoms of re-stenosis were observed within the follow-up period of 7 mo to 2 years. Further treatment with balloon dilatation or replacement of the biodegradable stent was not required.CONCLUSION: Biodegradable stents were useful for the treatment of benign esophageal stenosis, particularly for the prevention of post-ESD stenosis.

  8. Noncovalent interaction of polyethylene glycol with copper complex of ethylenediaminetetraacetic acid and its application in constructing inorganic nanomaterials.

    Science.gov (United States)

    Pan, Shu Zhen; Song, Le Xin; Chen, Jie; Du, Fang Yun; Yang, Jing; Xia, Juan

    2011-10-21

    In this study, we try to answer a fundamental question: what is the consequence of the noncovalent interaction between a polymer and a coordination compound? Here, polyethylene glycol (PEG-4000, PEG-b) and copper complex of ethylenediaminetetraacetic acid (H(2)CuY) were employed to solve this problem. A novel adduct (CEP) between H(2)CuY and PEG-b was prepared. Our results indicated several interesting findings. First, the introduction of H(2)CuY had no effect on the stacking structure of PEG-b but led to a large change in surface structure of the polymer. Second, there was a significant difference (117 K) in the maximum degradation temperature between the PEG and the CEP, suggesting that the noncovalent interaction can drastically improve the thermal stability of the PEG. Third, sintering experiments showed that H(2)CuY and CEP produced completely different decomposition products. The former formed Cu crystals in nitrogen and CuO in air, but the latter generated Cu and CuCl crystals with good crystallinity, respectively. Finally, three independent measurements: viscosity, conductivity and nuclear magnetic resonance in solution, provided useful information and insights from both sides of the noncovalent interaction. Probable interaction mechanisms and interaction sites were proposed. We consider that the current research could create the foundation for a new understanding of how the noncovalent adduct interaction between a metallic complex and a polymer relates to the change in physical and chemical properties of the adducted components.

  9. Construction of Prokaryotic Expressing Vector of Antisense Nucleic Acid of LasR and Its Effect on the Virulence of Pseudomonas Aeruginosus

    Institute of Scientific and Technical Information of China (English)

    ZHANG Ling; ZHOU Junli; LI Jingming; LIAO Fang

    2007-01-01

    To construct a pUCP18/lasRantisense plasmid carrying the reversed gene and analyze its effect on the virulence of Pseudomonas aeruginosus, LasR gene was amplified from the genome of Pseudomonas aeruginosus by PCR and reversely recombined with plasmid pUCP18. The recombinant pUCP18/lasRantisense was verified by enzyme digestion, PCR and sequencing. The biological effects of pUCP18/lasRantisense were examined by using RT-PCR, NAD method and the assay of pyocyanin. Our results showed that the expected full length lasR fragment (721 bp) was extended from Pseudomonas aeruginosus gene with PCR. And it is consistent with LasR gene of Pseudomonas aeruginosa in GenBank (No. NC_002516). The recombinant plasmid was successfully constructed and transferred into Pseudomonas aeruginosus. The antisense nucleic acid of LasR gene could reduce the virulence of Pseudomonas aeruginosus and might serve as a new target site for treatment purpose.

  10. Construction of a DOPC/PSM/cholesterol phase diagram based on the fluorescence properties of trans-parinaric acid.

    Science.gov (United States)

    Nyholm, Thomas K M; Lindroos, Daniel; Westerlund, Bodil; Slotte, J Peter

    2011-07-05

    Cell membranes have a nonhomogenous lateral organization. Most information about such nonhomogenous mixing has been obtained from model membrane studies where defined lipid mixtures have been characterized. Various experimental approaches have been used to determine binary and ternary phase diagrams for systems under equilibrium conditions. Such phase diagrams are the most useful tools for understanding the lateral organization in cellular membranes. Here we have used the fluorescence properties of trans-parinaric acid (tPA) for phase diagram determination. The fluorescence intensity, anisotropy, and fluorescence lifetimes of tPA were measured in bilayers composed of one to three lipid components. All of these parameters could be used to determine the presence of liquid-ordered and gel phases in the samples. However, the clearest information about the phase state of the lipid bilayers was obtained from the fluorescence lifetimes of tPA. This is due to the fact that an intermediate-length lifetime was found in samples that contain a liquid-ordered phase and a long lifetime was found in samples that contained a gel phase, whereas tPA in the liquid-disordered phase has a markedly shorter fluorescence lifetime. On the basis of the measured fluorescence parameters, a phase diagram for the 1,2-dioleoyl-sn-glycero-3-phosphocholine/N-palmitoyl sphingomyelin/cholesterol system at 23 °C was prepared with a 5 mol % resolution. We conclude that tPA is a good fluorophore for probing the phase behavior of complex lipid mixtures, especially because multilamellar vesicles can be used. The determined phase diagram shows a clear resemblance to the microscopically determined phase diagram for the same system. However, there are also significant differences that likely are due to tPA's sensitivity to the presence of submicroscopic liquid-ordered and gel phase domains.

  11. Synthesis, Structures and Photoluminescent Properties of Two Novel Zinc(II) Compounds Constructed from 5-Sulfoisophthalic Acid

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Yu Lan; Tang, Xue Ling; Ma, Kui Rong; Chen, Hao [Huaiyin Normal University, Huaian (China); Ma, Feng; Zhao, Lian Hua [Yanbian University, Yanji (China)

    2010-07-15

    Hydrothermal reaction of zinc(II) salts with 5-sulfoisophthalic acid monosodium salt (NaO{sub 3}SC{sub 6}H{sub 3}-1,3-(COOH){sub 2}, NaH{sub 2}- SIP) and 1,10-phenanthroline (phen) led to two new compounds, [Zn(phen){sub 3}]{center_dot}2H{sub 2}SIP{center_dot}4H{sub 2}O and [Zn(phen){sub 2}(H{sub 2}O){sub 2}]{center_dot} 2H{sub 2}SIP{center_dot}2H{sub 2}O. They were characterized by element analysis, IR spectroscopy, thermal gravimetric analysis (TGA), X-ray powder diffraction (XRD), and single-crystal X-ray diffraction. Both compounds 1-2 represent the first example of Zn/phen/SIP system. The Zn (II) ion in 1 is six-coordinated by six nitrogen atoms from three phen molecules, and the H{sub 2}SIP{sup -} ligands engage in the formation of hydrogen bond. The Zn(II) ion in 2 is coordinated by four nitrogen atoms from two phen molecules and two oxygen atoms from two water molecules. Moreover, both 1 and 2 are assembled into 3D supramolecular architectures by hydrogen bonds (O-H{center_dot}{center_dot}{center_dot}O) and {pi}-{pi} interactions. Solvent water molecules occupying voids of the compounds serve as receptors or donors of the extensive O-H{center_dot}{center_dot}{center_dot}O hydrogen bonds.

  12. Inhibitors of amino acids biosynthesis as antifungal agents.

    Science.gov (United States)

    Jastrzębowska, Kamila; Gabriel, Iwona

    2015-02-01

    Fungal microorganisms, including the human pathogenic yeast and filamentous fungi, are able to synthesize all proteinogenic amino acids, including nine that are essential for humans. A number of enzymes catalyzing particular steps of human-essential amino acid biosynthesis are fungi specific. Numerous studies have shown that auxotrophic mutants of human pathogenic fungi impaired in biosynthesis of particular amino acids exhibit growth defect or at least reduced virulence under in vivo conditions. Several chemical compounds inhibiting activity of one of these enzymes exhibit good antifungal in vitro activity in minimal growth media, which is not always confirmed under in vivo conditions. This article provides a comprehensive overview of the present knowledge on pathways of amino acids biosynthesis in fungi, with a special emphasis put on enzymes catalyzing particular steps of these pathways as potential targets for antifungal chemotherapy.

  13. Incorporation of extracellular fatty acids by a fatty acid kinase-dependent pathway in Staphylococcus aureus.

    Science.gov (United States)

    Parsons, Joshua B; Frank, Matthew W; Jackson, Pamela; Subramanian, Chitra; Rock, Charles O

    2014-04-01

    Acyl-CoA and acyl-acyl carrier protein (ACP) synthetases activate exogenous fatty acids for incorporation into phospholipids in Gram-negative bacteria. However, Gram-positive bacteria utilize an acyltransferase pathway for the biogenesis of phosphatidic acid that begins with the acylation of sn-glycerol-3-phosphate by PlsY using an acyl-phosphate (acyl-PO4 ) intermediate. PlsX generates acyl-PO4 from the acyl-ACP end-products of fatty acid synthesis. The plsX gene of Staphylococcus aureus was inactivated and the resulting strain was both a fatty acid auxotroph and required de novo fatty acid synthesis for growth. Exogenous fatty acids were only incorporated into the 1-position and endogenous acyl groups were channeled into the 2-position of the phospholipids in strain PDJ39 (ΔplsX). Extracellular fatty acids were not elongated. Removal of the exogenous fatty acid supplement led to the rapid accumulation of intracellular acyl-ACP and the abrupt cessation of fatty acid synthesis. Extracts from the ΔplsX strain exhibited an ATP-dependent fatty acid kinase activity, and the acyl-PO4 was converted to acyl-ACP when purified PlsX is added. These data reveal the existence of a novel fatty acid kinase pathway for the incorporation of exogenous fatty acids into S. aureus phospholipids.

  14. Construction and immunological evaluation of truncated hepatitis B core particles carrying HBsAg amino acids 119-152 in the major immunodominant region (MIR).

    Science.gov (United States)

    Su, Qiudong; Yi, Yao; Guo, Minzhuo; Qiu, Feng; Jia, Zhiyuan; Lu, Xuexin; Meng, Qingling; Bi, Shengli

    2013-09-13

    Hepatitis B capsid protein expressed in Escherichia coli can reassemble into icosahedral particles, which could strongly enhance the immunogenicity of foreign epitopes, especially those inserted into its major immunodominant region. Herein, we inserted the entire 'α' antigenic determinant amino acids (aa) 119-152 of HBsAg into the truncated HBc (aa 1-144), between Asp(78) and Pro(79). Prokaryotic expression showed that the mosaic HBc was mainly in the form of inclusion bodies. After denaturation with urea, it was dialyzed progressively for protein renaturation. We observed that before and after renaturation, mosaic HBc was antigenic as determined by HBsAg ELISA and a lot of viruslike particles were observed after renaturation. Thus, we further purified the mosaic viruslike particles by (NH4)2SO4 precipitation, DEAE chromatography, and Sepharose 4FF chromatography. Negative staining electron microscopy demonstrated the morphology of the viruslike particles. Immunization of Balb/c mice with mosaic particles induced the production of anti-HBs antibody and Th1 cell immune response supported by ELISPOT and CD4/CD8 proportions assay. In conclusion, we constructed mosaic hepatitis core particles displaying the entire 'α' antigenic determinant on the surface and laid a foundation for researching therapeutic hepatits B vaccines.

  15. Construction of a multiplex promoter reporter platform to monitor Staphylococcus aureus virulence gene expression and the identification of usnic acid as a potent suppressor of psm gene expression

    Directory of Open Access Journals (Sweden)

    Peng GAO

    2016-08-01

    Full Text Available As antibiotic resistance becomes phenomenal, alternative therapeutic strategies for bacterial infections such as anti-virulence treatments have been advocated. We have constructed a total of 20 gfp-luxABCDE dual-reporter plasmids with selected promoters from S. aureus virulence-associated genes. The plasmids were introduced into various S. aureus strains to establish a gfp-lux based multiplex promoter reporter platform for monitoring S. aureus virulence gene expressions in real time to identify factors or compounds that may perturb virulence of S. aureus. The gene expression profiles monitored by luminescence correlated well with qRT-PCR results and extrinsic factors including carbon dioxide and some antibiotics were shown to suppress or induce the expression of virulence factors in this platform. Using this platform, sub-inhibitory ampicillin was shown to be a potent inducer for the expression of many virulence factors in S. aureus. Bacterial adherence and invasion assays using mammalian cells were employed to measure S. aureus virulence induced by ampicillin. The platform was used for screening of natural extracts that perturb the virulence of S. aureus and usnic acid was identified to be a potent repressor for the expression of psm.

  16. Construction of a Multiplex Promoter Reporter Platform to Monitor Staphylococcus aureus Virulence Gene Expression and the Identification of Usnic Acid as a Potent Suppressor of psm Gene Expression.

    Science.gov (United States)

    Gao, Peng; Wang, Yanli; Villanueva, Iván; Ho, Pak Leung; Davies, Julian; Kao, Richard Yi Tsun

    2016-01-01

    As antibiotic resistance becomes phenomenal, alternative therapeutic strategies for bacterial infections such as anti-virulence treatments have been advocated. We have constructed a total of 20 gfp-luxABCDE dual-reporter plasmids with selected promoters from S. aureus virulence-associated genes. The plasmids were introduced into various S. aureus strains to establish a gfp-lux based multiplex promoter reporter platform for monitoring S. aureus virulence gene expressions in real time to identify factors or compounds that may perturb virulence of S. aureus. The gene expression profiles monitored by luminescence correlated well with qRT-PCR results and extrinsic factors including carbon dioxide and some antibiotics were shown to suppress or induce the expression of virulence factors in this platform. Using this platform, sub-inhibitory ampicillin was shown to be a potent inducer for the expression of many virulence factors in S. aureus. Bacterial adherence and invasion assays using mammalian cells were employed to measure S. aureus virulence induced by ampicillin. The platform was used for screening of natural extracts that perturb the virulence of S. aureus and usnic acid was identified to be a potent repressor for the expression of psm.

  17. Synthesis, structures, and fluorescence properties of two d-d heterometallic cluster-based complexes constructed by N-(phosphonomethyl) iminodiacetic acid

    Science.gov (United States)

    Li, Sa-Ying

    2016-12-01

    Two novel d-d heterometallic cluster-based complexes constructed by N-(Phosphonomethyl)imino-diacetic acid (H4PMIDA) multifunctional ligand, [Cu2Zn2(PMIDA)2(H2O)3]·3H2O(1), [ZnNi14(PMIDA)6(H2O)18]·(NO3)6·15H2O(2), have been synthesized under hydrothermal conditions and characterized by elemental analyses, IR spectra, thermal analyses, and single-crystal X-ray diffraction. The complex 1 is one-dimensional heteronuclear molecular chain, which is further extends into a 3D supramolecular network through very extensive Osbnd H⋯O hydrogen bonds. The compound 2 is a rare novel zero-dimensional heteronuclear molecular cluster, which is further extends into a 3D supramolecular network through very extensive Osbnd H⋯O hydrogen bonds. Moreover, the solid-state fluorescence properties of the two complexes have also been investigated at room temperature.

  18. Synthesis, structures of four coordination compounds constructed from o-methacrylamidobenzoic acid and their relationship between structure and solid state luminescence

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Hong-Xia; Ma, Yong; Zhou, Feng; Wu, Bing [Key Laboratory of Organic Synthesis of Jiangsu Province, School of Chemistry, Chemical Engineering and Materials Science, Soochow University(DuShuHu Campus), 199 Renai Road, Suzhou, 215123 (China); Key Laboratory of Energy-Saving And Environmental Protection Materials Test and Technical Service Center of Jiangsu Province, Soochow University (DuShuHu Campus), 199 Renai Road, Suzhou, 215123 (China); Xu, Qing-Feng, E-mail: xuqingfeng@suda.edu.cn [Key Laboratory of Organic Synthesis of Jiangsu Province, School of Chemistry, Chemical Engineering and Materials Science, Soochow University(DuShuHu Campus), 199 Renai Road, Suzhou, 215123 (China); Key Laboratory of Energy-Saving And Environmental Protection Materials Test and Technical Service Center of Jiangsu Province, Soochow University (DuShuHu Campus), 199 Renai Road, Suzhou, 215123 (China); Lu, Jian-Mei, E-mail: lujm@suda.edu.cn [Key Laboratory of Organic Synthesis of Jiangsu Province, School of Chemistry, Chemical Engineering and Materials Science, Soochow University(DuShuHu Campus), 199 Renai Road, Suzhou, 215123 (China); Key Laboratory of Energy-Saving And Environmental Protection Materials Test and Technical Service Center of Jiangsu Province, Soochow University (DuShuHu Campus), 199 Renai Road, Suzhou, 215123 (China); Ge, Jian-Feng [Key Laboratory of Organic Synthesis of Jiangsu Province, School of Chemistry, Chemical Engineering and Materials Science, Soochow University(DuShuHu Campus), 199 Renai Road, Suzhou, 215123 (China); Key Laboratory of Energy-Saving And Environmental Protection Materials Test and Technical Service Center of Jiangsu Province, Soochow University (DuShuHu Campus), 199 Renai Road, Suzhou, 215123 (China)

    2013-07-15

    Four new coordination compounds, namely, Zn(o-MAABA){sub 2}(Phen) (1), [Cd(o-MAABA){sub 2}·2H{sub 2}O]{sub 2} (2), ([Pb{sub 2}Cl{sub 2}(o-MAABA){sub 2}(Phen){sub 4}])·2H{sub 2}O (3·2H{sub 2}O), [Pb(NO{sub 3})(o-MAABA)(Phen)]{sub n} (4), where o-MAABA=o-methacrylamidobenzoic acid and phen=1, 10-phenanthroline, have been synthesized. All compounds were fully confirmed by FT-IR, elemental analysis and TGA analysis. Their structures were determined by single crystal X-ray diffraction, in which compound 1 shows a mononuclear structure, compounds 2 and 3 have binuclear structures and compound 4 shows an infinite chain. In 2 and 4, the adjacent chains are extended into a 3D supramolecular architecture via π–π interactions. Solid-state room temperature luminescence spectra revealed that emission bands of compound 1 were located at 524 nm (λ{sub ex}=352 nm) and compound 4 at 479 and 584 nm (λ{sub ex}=390 nm) assigned to the excimer formation. The emission at 454 nm (λ{sub ex}=340 nm) of compound 2 was mainly ascribed to the Ligand–Metal Charge Transfer (LMCT). - Graphical abstract: Four coordination compounds constructed by o-methacrylamidobenzoic, phenanthroline and metal ions are reported. The photoluminescent properties is studied, which is affected by the molecular stacking and LMCT.

  19. Three new Cu(Ⅱ)-Ln(Ⅲ)heterometallic coordination polymers constructed from quinolinic acid and nicotinic acid:Synthesis,structures,and magnetic properties%Three new Cu(Ⅱ)-Ln(Ⅲ)heterometallic coordination polymers constructed from quinolinic acid and nicotinic acid: Synthesis,structures,and magnetic properties

    Institute of Scientific and Technical Information of China (English)

    LIU SuiJun; SONG WeiChao; XUE Li; HAN SongDe; ZENG YongFei; WANG LiFu; BU XianHe

    2012-01-01

    Three new Cu(Ⅱ)-Ln(Ⅲ)heterometallic coordination polymers based on two N-heterocyclic carboxyllc ligands,{[LnCu(L1)2(L2)(H2O)2]· mH2O}n(Ln =La(1),Nd(2),Gd(3),m =2(for 1),1(for 2,3),H2L1 =quinolinic acid,HL2 =nicotinic acid),have been synthesized and characterized.1 has a two-dimensional(2D)layer structure with a Schl(a)fli symbol of(44.62),while complexes 2 and 3 are isostructural and have three-dimensional(3D)structures with a Schl(a)fli symbol of(3.4.5)2(32.42.52.614.74.83.9)(32.63.7)of 3-nodal net.Magnetic investigations suggest that antiferromagnetic coupling exists between NdⅢ and CuⅡ in 2,while weak ferromagnetic coupling between GdⅢ and CuⅡ in 3.The difference of magnetic prooerties between 2 and 3 has been discussed.

  20. Oxygen-dependent catabolism of indole-3-acetic acid in Bradyrhizobium japonicum

    DEFF Research Database (Denmark)

    Egebo, L A; Nielsen, S V; Jochimsen, B U

    1991-01-01

    addition of the substrates for induction and is oxygen dependent. The highest activity is obtained when the concentration of inducer is 0.2 mM. Spectrophotometric data are consistent with the suggestion that the indole ring is broken during degradation of IAA. We hypothesize that the enzyme catalyzes...... an oxygen-consuming opening of the indole ring analogous to the one catalyzed by tryptophan 2,3-dioxygenase. The pattern of metabolite usage by known tryptophan-auxotrophic mutants and studies of metabolites by high-performance liquid chromatography indicate that anthranilic acid is a terminal degradation...

  1. Construction and immunological evaluation of truncated hepatitis B core particles carrying HBsAg amino acids 119–152 in the major immunodominant region (MIR)

    Energy Technology Data Exchange (ETDEWEB)

    Su, Qiudong; Yi, Yao [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China); Guo, Minzhuo [Beijing Entry-Exit Inspection and Quarantine Beureau, Tianshuiyuan Lane 6, Chaoyang District, Beijing 100026 (China); Qiu, Feng; Jia, Zhiyuan; Lu, Xuexin; Meng, Qingling [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China); Bi, Shengli, E-mail: shengli_bi@163.com [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China)

    2013-09-13

    Highlights: •The conformational HBV neutralization antigen domain was successfully displayed on the surface of truncated HBc particles. •Appropriate dialysis procedures to support the renaturing environment for the protein refolding. •Efficient purification procedures to obtain high purity and icosahedral particles of mosaic HBV antigen. •Strong immune responses not only including neutralization antibody response but also Th1 cell response were induced in mice. -- Abstract: Hepatitis B capsid protein expressed in Escherichia coli can reassemble into icosahedral particles, which could strongly enhance the immunogenicity of foreign epitopes, especially those inserted into its major immunodominant region. Herein, we inserted the entire ‘α’ antigenic determinant amino acids (aa) 119–152 of HBsAg into the truncated HBc (aa 1–144), between Asp{sup 78} and Pro{sup 79}. Prokaryotic expression showed that the mosaic HBc was mainly in the form of inclusion bodies. After denaturation with urea, it was dialyzed progressively for protein renaturation. We observed that before and after renaturation, mosaic HBc was antigenic as determined by HBsAg ELISA and a lot of viruslike particles were observed after renaturation. Thus, we further purified the mosaic viruslike particles by (NH{sub 4}){sub 2}SO{sub 4} precipitation, DEAE chromatography, and Sepharose 4FF chromatography. Negative staining electron microscopy demonstrated the morphology of the viruslike particles. Immunization of Balb/c mice with mosaic particles induced the production of anti-HBs antibody and Th1 cell immune response supported by ELISPOT and CD4/CD8 proportions assay. In conclusion, we constructed mosaic hepatitis core particles displaying the entire ‘α’ antigenic determinant on the surface and laid a foundation for researching therapeutic hepatits B vaccines.

  2. Three two-dimensional coordination polymers constructed from transition metals and 2,3-norbornanedicarboxylic acid: Hydrothermal synthesis, crystal structures and photocatalytic properties

    Science.gov (United States)

    Zhang, Jia; Wang, Chong-Chen

    2017-02-01

    Three novel coordination polymers based on transition metals like Co(II), Cu(II) and Mn(II), namely [Co2(bpy)2(nbda)2(H2O)2]·2H2O (denoted as BUC-1), [Cu2(bpy)2(nbda)2(H2O)2]·2H2O (BUC-2), [Mn2(bpy)2(nbda)2(H2O)2]·2H2O (BUC-3), (where bpy = 4,4‧-bipyridine, H2nbda = 2,3-norbornanedicarboxylic acid, BUC = Beijing University of Civil Engineering and Architecture), were synthesized under hydrothermal conditions, and characterized by CNH elemental analyses (EA), Fourier Transform infrared spectroscopy (FTIR), and single crystal X-ray diffraction (SCXRD). BUC 1-3 were isostructural and crystallized in the monoclinic space group C2/c, in which the corresponding metal atoms were linked by typical bidentate bpy ligands into two adjacent 1D [M1(bpy)]n2n+ and [M2(bpy)]n2n+ (M = Co(II), Cu(II), Mn(II)), further joined by versatile nbda2- ligands into 2D [M2(bpy)2(nbda)2]n sheets. Finally, three-dimensional supramolecular frameworks were constructed with the aid of the intermolecular hydrogen bonding interactions. BUC 1-3 exhibited different photocatalytic degradation ability to decompose methylene blue (MB) and methyl orange (MO) under UV light irradiation. Additionally, a possible photocatalytic mechanism HOMO-LUMO was proposed and discussed, which was further confirmed by radicals trapping experiments using isopropanol as radical scavenger.

  3. Availability of Amino Acids Extends Chronological Lifespan by Suppressing Hyper-Acidification of the Environment in Saccharomyces cerevisiae.

    Directory of Open Access Journals (Sweden)

    Yo Maruyama

    Full Text Available The chronological lifespan of Saccharomyces cerevisiae represents the duration of cell survival in the postdiauxic and stationary phases. Using a prototrophic strain derived from the standard auxotrophic laboratory strain BY4742, we showed that supplementation of non-essential amino acids to a synthetic defined (SD medium increases maximal cell growth and extends the chronological lifespan. The positive effects of amino acids can be reproduced by modulating the medium pH, indicating that amino acids contribute to chronological longevity in a cell-extrinsic manner by alleviating medium acidification. In addition, we showed that the amino acid-mediated effects on extension of chronological longevity are independent of those achieved through a reduction in the TORC1 pathway, which is mediated in a cell-intrinsic manner. Since previous studies showed that extracellular acidification causes mitochondrial dysfunction and leads to cell death, our results provide a path to premature chronological aging caused by differences in available nitrogen sources. Moreover, acidification of culture medium is generally associated with culture duration and cell density; thus, further studies are required on cell physiology of auxotrophic yeast strains during the stationary phase because an insufficient supply of essential amino acids may cause alterations in environmental conditions.

  4. Palladium-Catalyzed Asymmetric Conjugate Addition of Arylboronic Acids to Five-, Six-, and Seven-Membered β-Substituted Cyclic Enones: Enantioselective Construction of All-Carbon Quaternary Stereocenters

    KAUST Repository

    Kikushima, Kotaro

    2011-05-11

    The first enantioselective Pd-catalyzed construction of all-carbon quaternary stereocenters via 1,4-addition of arylboronic acids to β-substituted cyclic enones is reported. Reaction of a wide range of arylboronic acids and cyclic enones using a catalyst prepared from Pd(OCOCF(3))(2) and a chiral pyridinooxazoline ligand yields enantioenriched products bearing benzylic stereocenters. Notably, this transformation is tolerant to air and moisture, providing a practical and operationally simple method of synthesizing enantioenriched all-carbon quaternary stereocenters.

  5. Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Applied to Quantitative Proteomics of Bacillus subtilis

    DEFF Research Database (Denmark)

    Soufi, Boumediene; Kumar, C.; Gnad, F.

    2010-01-01

    We applied stable isotope labeling by amino acids in cell culture (SILAC) to large-scale quantitative proteomics analyses of the model bacterium Bacillus subtilis in two physiological conditions: growth on succinate and growth under phosphate starvation. Using a B. subtilis strain auxotrophic...... of the most comprehensive quantitative proteomics studies in bacteria, covering more than 75% of the B. subtilis genes expressed in the log phase of growth. Furthermore, we detect and quantify dynamics of 35 Ser/Thr/Tyr phosphorylation sites under growth on succinate, and 10 phosphorylation sites under...

  6. Two new coordination polymers constructed by naphthalene-1,4-dicarboxylic acid and 2,4-diamino-6-methyl-triazine

    Energy Technology Data Exchange (ETDEWEB)

    Li, Yamin, E-mail: liyamin@henu.edu.cn [Institute of Molecular and Crystal Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004 (China); Xiao, Changyu [Institute of Molecular and Crystal Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004 (China); Zhang, Xudong [State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China); Xu, Yanhui [Department of Medical Imaging, Bethune Medical Non-Commissioned Officer' s, College, Shijiazhuang, Hebei 050081 (China); Li, Junrui; Lun, Huijie; Chen, Qi [Institute of Molecular and Crystal Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004 (China)

    2013-08-15

    Two new transition metal coordination complexes, ([MnO(nda)](H{sub 2}dmt)(H{sub 2}O)){sub n} (1), [Ag{sub 5}(nda){sub 2.5}(dmt)]{sub n} (2), (H{sub 2}nda=naphthalene-1,4-dicarboxylic acid, dmt=2,4-diamine-6-methyl-1,3,5-triazine) have been hydrothermally synthesized by the reactions of H{sub 2}nda and dmt with the homologous MnCl{sub 2}·4H{sub 2}O and AgNO{sub 3}, respectively, and characterized by single-crystal X-ray diffraction, IR spectra, elemental analysis, thermogravimetric analysis (TGA). The compound 1 exhibits a 3D network comprising 1D metal chain (MnO(CO{sub 2}){sub 2}){sub n} connected by the ligand nda{sup 2−}, featuring a four-connected uninodal diamond -like topology. In compound 2, it is firstly observed that decanuclear silver units as secondary building units to construct 3D network by the ligands dmt and nda{sup 2−}, with a rare 2-nodal (3,8)-connected tfz-d topology ((4{sup 3}){sub 2}(4{sup 6}.6{sup 18}.8{sup 4})). The interactions within each Mn(II)—Mn(II) pair of compound 1 are antiferromagnetic (g=2.07, J=−1.42(1) cm{sup −1}, zj′=−0.73(2) cm{sup −1}). In addition, compound 2 exhibits photoluminescent property at about 472 nm (λ{sub ex}=394 nm). - Graphical abstract: Two new transition metal coordination complexes 1–2 have been hydrothermally synthesized and characterized by single-crystal X-ray diffraction, IR spectra, elemental analysis thermogravimetric analysis (TGA). Highlights: • The compound 1 exhibits a 3D network with four-connected uninodal diamond-like topology. • The first 3D network of 2 with a rare tfz-d topology consists of decanuclear silver clusters as secondary building units. • The magnetic measurement indicates the compound 1 shows antiferromagnetic interactions. • The photoluminescent property of 2 has been measured.

  7. 透明质酸改性聚乳酸支架组织工程软骨的构建%Construction of tissue engineering cartilage with modified polylactic acid scaffold using hyaluronic acid

    Institute of Scientific and Technical Information of China (English)

    刘春栋; 张志光; 苏凯; 申晓青

    2012-01-01

    Objective To construct tissue engineering neocartialge based on the modified polylactic acid (PLA) scaffold with hyaluronic acid ( HA). The significance of HA in construct tissue-engineered cartilage was to be analyzed and discussed. Methods The highly porous PLA scaffold was made by salt-leaching method; Low concentration NaOH, car-bodiimide and HA were used to modify the scaffold. The third passage of chondrocytes was seeded into the scaffold after prewetted. The concentration was 5 ×107 per milliliter. The complex was implanted into rabbits one week after vitro culture. The prepared complex was implanted into the back of the New Zealand rabbits. The complexes of chondrocyte-modi-fied PLA scaffold were applied in the experimental group, and the complexes of chondrocyte-PLA scaffold were used in the control group. Two rabbits were sacrificed randomly at four, six, eight weeks after implantation. The tissue were taken out according to the marker made during operation at 4, 6, 8 weeks after operation respectively. The histologioal characteristics was observed by HE, Masson, toluidine blue stain and type Ⅱ collagen immunohistochemical test. Results There was no immunoreactive rejection after implantation of the cell-scaffold complex. After two month two kinds of cell-scaffold appearance were similar to the articular cartilage. The results of histochemical test revealed that the tissue had been becoming better when being ompanted longer. A comparison analysis was made according to the expression intensity using Imagej. There were statistical significance between experimental and control groups (P < 0.05) at 6, 8 weeks. The secretion cartilage matrix of modified scaffold group was better than that of the PLA group. Conclusion The allogeneic chondrocytes could be the seed of tissue-engineered cartilage. The modified PLA scaffold can be the carrier to form neo-cartilage in vivo.%目的 利用透明质酸( hyaluronic acid,HA)改性聚乳酸(polylactic acid,PLA)支架

  8. Construction management

    CERN Document Server

    Pellicer, Eugenio; Teixeira, José C; Moura, Helder P; Catalá, Joaquín

    2014-01-01

    The management of construction projects is a wide ranging and challenging discipline in an increasingly international industry, facing continual challenges and demands for improvements in safety, in quality and cost control, and in the avoidance of contractual disputes. Construction Management grew out of a Leonardo da Vinci project to develop a series of Common Learning Outcomes for European Managers in Construction. Financed by the European Union, the project aimed to develop a library of basic materials for developing construction management skills for use in a pan-European context. Focused exclusively on the management of the construction phase of a building project from the contractor's point of view, Construction Management covers the complete range of topics of which mastery is required by the construction management professional for the effective delivery of new construction projects. With the continued internationalisation of the construction industry, Construction Management will be required rea...

  9. The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering.

    Science.gov (United States)

    Abdul Rahman, Rozlin; Mohamad Sukri, Norhamiza; Md Nazir, Noorhidayah; Ahmad Radzi, Muhammad Aa'zamuddin; Zulkifly, Ahmad Hafiz; Che Ahmad, Aminudin; Hashi, Abdurezak Abdulahi; Abdul Rahman, Suzanah; Sha'ban, Munirah

    2015-08-01

    Articular cartilage is well known for its simple uniqueness of avascular and aneural structure that has limited capacity to heal itself when injured. The use of three dimensional construct in tissue engineering holds great potential in regenerating cartilage defects. This study evaluated the in vitro cartilaginous tissue formation using rabbit's bone marrow mesenchymal stem cells (BMSCs)-seeded onto poly(lactic-co-glycolic acid) PLGA/fibrin and PLGA scaffolds. The in vitro cartilaginous engineered constructs were evaluated by gross inspection, histology, cell proliferation, gene expression and sulphated glycosaminoglycan (sGAG) production at week 1, 2 and 3. After 3 weeks of culture, the PLGA/fibrin construct demonstrated gross features similar to the native tissue with smooth, firm and glistening appearance, superior histoarchitectural and better cartilaginous extracellular matrix compound in concert with the positive glycosaminoglycan accumulation on Alcian blue. Significantly higher cell proliferation in PLGA/fibrin construct was noted at day-7, day-14 and day-21 (p<0.05 respectively). Both constructs expressed the accumulation of collagen type II, collagen type IX, aggrecan and sox9, showed down-regulation of collagen type I as well as produced relative sGAG content with PLGA/fibrin construct exhibited better gene expression in all profiles and showed significantly higher relative sGAG content at each time point (p<0.05). This study suggested that with optimum in vitro manipulation, PLGA/fibrin when seeded with pluripotent non-committed BMSCs has the capability to differentiate into chondrogenic lineage and may serve as a prospective construct to be developed as functional tissue engineered cartilage.

  10. Design and characterization of auxotrophy-based amino acid biosensors.

    Directory of Open Access Journals (Sweden)

    Felix Bertels

    Full Text Available Efficient and inexpensive methods are required for the high-throughput quantification of amino acids in physiological fluids or microbial cell cultures. Here we develop an array of Escherichia coli biosensors to sensitively quantify eleven different amino acids. By using online databases, genes involved in amino acid biosynthesis were identified that - upon deletion - should render the corresponding mutant auxotrophic for one particular amino acid. This rational design strategy suggested genes involved in the biosynthesis of arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tryptophan, and tyrosine as potential genetic targets. A detailed phenotypic characterization of the corresponding single-gene deletion mutants indeed confirmed that these strains could neither grow on a minimal medium lacking amino acids nor transform any other proteinogenic amino acid into the focal one. Site-specific integration of the egfp gene into the chromosome of each biosensor decreased the detection limit of the GFP-labeled cells by 30% relative to turbidometric measurements. Finally, using the biosensors to determine the amino acid concentration in the supernatants of two amino acid overproducing E. coli strains (i.e. ΔhisL and ΔtdcC both turbidometrically and via GFP fluorescence emission and comparing the results to conventional HPLC measurements confirmed the utility of the developed biosensor system. Taken together, our study provides not only a genotypically and phenotypically well-characterized set of publicly available amino acid biosensors, but also demonstrates the feasibility of the rational design strategy used.

  11. Usability Constructs

    DEFF Research Database (Denmark)

    Hertzum, Morten; Clemmesen, Torkil; Hornbæk, Kasper Anders Søren

    2007-01-01

    Whereas research on usability predominantly employs universal definitions of the aspects that comprise usability, people experience their use of information systems through personal constructs. Based on 48 repertory-grid interviews, this study investigates how such personal constructs are affecte...

  12. An amino acid depleted cell-free protein synthesis system for the incorporation of non-canonical amino acid analogs into proteins.

    Science.gov (United States)

    Singh-Blom, Amrita; Hughes, Randall A; Ellington, Andrew D

    2014-05-20

    Residue-specific incorporation of non-canonical amino acids into proteins is usually performed in vivo using amino acid auxotrophic strains and replacing the natural amino acid with an unnatural amino acid analog. Herein, we present an efficient amino acid depleted cell-free protein synthesis system that can be used to study residue-specific replacement of a natural amino acid by an unnatural amino acid analog. This system combines a simple methodology and high protein expression titers with a high-efficiency analog substitution into a target protein. To demonstrate the productivity and efficacy of a cell-free synthesis system for residue-specific incorporation of unnatural amino acids in vitro, we use this system to show that 5-fluorotryptophan and 6-fluorotryptophan substituted streptavidin retain the ability to bind biotin despite protein-wide replacement of a natural amino acid for the amino acid analog. We envisage this amino acid depleted cell-free synthesis system being an economical and convenient format for the high-throughput screening of a myriad of amino acid analogs with a variety of protein targets for the study and functional characterization of proteins substituted with unnatural amino acids when compared to the currently employed in vivo methodologies.

  13. Electrochemical construction

    Science.gov (United States)

    Einstein, Harry; Grimes, Patrick G.

    1983-08-23

    An electrochemical cell construction features a novel co-extruded plastic electrode in an interleaved construction with a novel integral separator-spacer. Also featured is a leak and impact resistant construction for preventing the spill of corrosive materials in the event of rupture.

  14. 15种氨基酸随机肽库的小盒式DNA编码文库的构建%Construction of a DNA Library in Small Cassette Encoding Random Peptide Consisting of 15 Amino Acids

    Institute of Scientific and Technical Information of China (English)

    卢明锋; 康寿凯; 张月杰; 张红雨

    2012-01-01

    采用小盒式DNA编码文库的构建策略,选取在进化上可能起源较早的15种氨基酸,按照其简并密码子合成了一个为10个随机氨基酸编码的小盒式DNA模板,经过连续3轮的PCR扩增、酶切及连接的小盒式文库组装过程,成功构建了一个文库容量达1.31×1012/ml,随机编码区长达97个氨基酸的小盒式DNA编码文库.%Using a small cassette DNA encoding library construction strategies, DNA template encoding 10 random amino acids was synthesized according to degenerate codon of a 15 kinds amino acids set. After three consecutive small cassette library assembly process including PCR amplification, digestion and connection, a small cassette DNA encoding library which a random coding region with length up to 97 amino acids was successfully constructed, and library capacity was 1.31×1012 /ml.

  15. Induced Mutation Breeding of Gomphidius viscidus Amino Acid Auxotrophic Strain by UV%血红铆钉菇氨基酸缺陷型菌株的紫外诱变选育

    Institute of Scientific and Technical Information of China (English)

    勾丽莉; 李莉; 周建树; 孟庆国; 关艳丽; 赵洁

    2008-01-01

    通过原生质体去细胞壁技术,以血红铆钉菇原生质体为材料,利用紫外线对其进行诱变处理,筛选出6株氨基酸营养缺陷型突变株,经稳定性试验确认1株突变株性状可以稳定遗传,利用生长谱法对缺陷型进行了鉴定、分析.结果表明,该菌株为L-半胱氨酸缺陷型菌株,为营养缺陷型突变株的筛选奠定了基础.

  16. Application of Health Belief Model\\'s constructs for predicting regular consumption of folic acid supplements in pregnant women referred to Borazjan’s health centers in 2014-15

    Directory of Open Access Journals (Sweden)

    Zohre kafaee

    2016-04-01

    Full Text Available Background: One of the most common disorders in pregnancy is Folic Acid deficiency and its complications. The aim of this study was to examine the predictors of regular use of folic acid supplements based on HBM in pregnant women referred to Borazjan’s health centers. Material and Methods: In this cross-sectional study, 228 pregnant women or women with planning of pregnancy referred to health centers of Borazjan with random sampling method evaluated. Data was collected with questionnaire in 4 parts included demographic characteristics, knowledge, health belief model constructs and questions about folic acid supplement use. Data was analyzed by SPSS software with using appropriate statistical tests. Results: The mean age of samples was 27.4±5.41. 144 patients (63.2% consumed Folic Acid pills regularly, and 84 patients (36.8% had irregular use. The awareness of folic acid in 22.8% of women was good, 59.6%  and 17.5% of samples had intermediate and poor awareness, respectively. The perceived barriers (P<0.001, perceived benefits (P=0.002 and self-efficacy (P<0.001 had relation with consumption of folic acid and among demographic variables, only education level (P=0.04 had relation with the consumption of pills. In logistic regression perceived barriers was only predictor. Age and educational level had indirect effect in regular consume pill. Conclusion: Perceived barriers was strongest predictors of folic acid use, therefore intervention based on health belief model, with emphasis on reducing barriers is necessary for improving the use of this medicine during pregnancy.

  17. Factors enhancing L-valine production by the growth-limited L-isoleucine auxotrophic strain Corynebacterium glutamicum DeltailvA DeltapanB ilvNM13 (pECKAilvBNC).

    Science.gov (United States)

    Denina, Ilze; Paegle, Longina; Prouza, Marek; Holátko, Jiri; Pátek, Miroslav; Nesvera, Jan; Ruklisha, Maija

    2010-07-01

    Cell growth limitation is known to be an important condition that enhances L: -valine synthesis in Corynebacterium glutamicum recombinant strains with L: -isoleucine auxotrophy. To identify whether it is the limited availability of L: -isoleucine itself or the L: -isoleucine limitation-induced rel-dependent ppGpp-mediated stringent response that is essential for the enhancement of L: -valine synthesis in growth-limited C. glutamicum cells, we deleted the rel gene, thereby constructing a relaxed (rel (-) ) C. glutamicum DeltailvA DeltapanB Deltarel ilvNM13 (pECKAilvBNC) strain. Variations in enzyme activity and L: -valine synthesis in rel (+) and rel (-) strains under conditions of L: -isoleucine excess and limitation were investigated. A sharp increase in acetohydroxy acid synthase (AHAS) activity, a slight increase in acetohydroxyacid isomeroreductase (AHAIR) activity, and a dramatic increase in L: -valine synthesis were observed in both rel (+) and rel (-) cells exposed to L: -isoleucine limitation. Although the positive effect of induction of the stringent response on AHAS and AHAIR upregulation in cells was not confirmed, we found the stringent response to be beneficial for maintaining increased AHAS, dihydroxyacid dehydratase, and transaminase B activity and L: -valine synthesis in cells during the stationary growth phase.

  18. Constructive Fun.

    Science.gov (United States)

    Simanek, Donald E.

    1994-01-01

    Compares and reviews currently available brands of steel construction sets that are useful to physics teachers for building demonstrations, prototypes of mechanisms, robotics, and remote control devices. (ZWH)

  19. TEM and SEM observations of super-structures constructed in organogel systems from a combination of boronic-acid-appended bola-amphiphiles with chiral diols

    Science.gov (United States)

    Koumoto, Kazuya; Yamashita, Tadahiro; Kimura, Taro; Luboradzki, Roman; Shinkai, Seiji

    2001-03-01

    Two bola-amphiphilic α,ω-diboronic acids separated by a (CH2)11 or (CH2)12 group were synthesized. Complexation with chiral diols readily gave new amphiphiles end-capped with the chiral substituents. Some of these acted as good gelators of organic solvents. Transmission electron microscope and scanning electron microscope observations established that a variety of super-structures are created in the organogels, depending on the solvents and the structure of the chiral end-cap groups. In most cases, the fibrous aggregates, the network structure which is the driving-force for gelation, showed a helical higher-order structure reflecting the chirality of the end-cap groups. The results indicate that the combinatorial approach utilizing boronic acid functions and diol compounds is useful in creating a variety of new super-structures in the gel phase.

  20. Synthesis and Crystal Structure of a Two-dimensional Silver Framework Constructed by H2idc (H2idc = Iminodiacetic Acid)

    Institute of Scientific and Technical Information of China (English)

    LIN Xiu-E; CAO Rong; WANG Yan-Qin; LI Xiao-Ju; BI Wen-Hua; YUAN Da-Qiang

    2005-01-01

    A new compound formulated [Ag2(idc)](NO3)(H2O (H2idc = iminodiacetic acid) was synthesized under mild conditions and characterized by IR spectrum and single-crystal X-ray diffraction. The crystal crystallizes in the monoclinic system, space group of P21/n with a = 5.5029(2), b = 11.1316(5), c = 15.5319 (1) (A), β = 96.038(3)°, V = 946.15(6) (A)3, Z = 4, R = 0.0619 and wR = 0.1606. Its structure is a 2D polymer, whose repeat units consist of dinuclear silver cluster coordinated by two bridging iminodiacetic acids. In the complex, the Ag(I) ions adopt three coordinate fashions, and the Ag-Ag metal-metal contacts of 2.8418 (A) are observed.

  1. Seq2Logo: a method for construction and visualization of amino acid binding motifs and sequence profiles including sequence weighting, pseudo counts and two-sided representation of amino acid enrichment and depletion

    DEFF Research Database (Denmark)

    Thomsen, Martin Christen Frølund; Nielsen, Morten

    2012-01-01

    Seq2Logo is a web-based sequence logo generator. Sequence logos are a graphical representation of the information content stored in a multiple sequence alignment (MSA) and provide a compact and highly intuitive representation of the position-specific amino acid composition of binding motifs, active...... sites, etc. in biological sequences. Accurate generation of sequence logos is often compromised by sequence redundancy and low number of observations. Moreover, most methods available for sequence logo generation focus on displaying the position-specific enrichment of amino acids, discarding the equally...... valuable information related to amino acid depletion. Seq2logo aims at resolving these issues allowing the user to include sequence weighting to correct for data redundancy, pseudo counts to correct for low number of observations and different logotype representations each capturing different aspects...

  2. Regulated-stereoselective construction of thirteen stereogenic centers necessary for the frame of (+)-discodermolide, based on iterative Lewis acid-promoted aldol reactions.

    Science.gov (United States)

    Kiyooka, Syun-ichi; Shahid, Kazi Abdus; Goto, Fumitaka; Okazaki, Momotoshi; Shuto, Yoshihiro

    2003-10-17

    The segments C(1)-C(13) and C(15)-C(21) containing the 13 stereogenic centers required for the frame of (+)-discodermolide were synthesized in good to excellent enantio- and diastereoselectivities from a common racemic aldehyde, derived from 2-methyl-1,3-propanediol. The enantioselective aldol reactions of the racemic aldehyde with a silylketene acetal, derived from ethyl 2-bromopropionate, in the presence of chiral oxazaborolidinones, prepared in situ with N-p-toluenesulfonyl-(R)- and -(S)-valine and BH(3).THF, proceeded under kinetic control to give the stereotriads with a high degree of enantioselectivity. Enantioselective (chiral borane) and diastereoselective (BF(3).OEt(2) and TiCl(4)) aldol reactions with the silylketene acetal, coupled with diastereoselective radical debrominations (Bu(3)SnH, Et(3)B, with or without MgBr(2)), were used iteratively. This aldol reaction strategy for the construction of the polypropionate frame dramatically shortened the steps needed for the construction of the final segments.

  3. Synthesis, crystal structure and photoelectric property of two new coordination polymers constructed by longer-spanning suberic acid and 4,4'-bipyridine ligands.

    Science.gov (United States)

    Xie, Yan; Bai, Feng Ying; Li, Jing; Xing, Yong Heng; Wang, Zhuo; Zhao, Hai Yan; Pu, Zhi Feng; Ge, Mao Fa; Shi, Zhan

    2010-11-01

    Two-dimensional coordination polymers, [M(C₈H₁₂O₄)(C₁₀H₈N₂)]·H₂O [M=Co (1), Cd (2); C₁₀H₈N₂ = 4,4-bipyridine, C₈H₁₄O₄=subaric acid] were obtained from the reaction of the metal salts, bipy and subaric acid at 180°C and characterized by elemental analysis, infrared spectrum, and single-crystal X-ray diffraction and surface photovoltage spectrum (SPS). The single-crystal X-ray diffraction showed that the subaric ligand in the two complexes exhibits two types of modes coordinating to transition metal ions, resulting in the formation of a 1D infinite chain along the c-axis. In addition, the results of SPS for complexes 1 and 2 indicate that these two complexes exhibit positive surface photovoltage responses in the range of 300-800 nm, which can be assigned to LMCT and MLCT, respectively. And the SPS of complex 1 also can be assigned to the d→d* electronic transition. The SPS spectra of the two complexes are consistent with their UV-vis spectra.

  4. Construction aggregates

    Science.gov (United States)

    Nelson, T.I.; Bolen, W.P.

    2007-01-01

    Construction aggregates, primarily stone, sand and gravel, are recovered from widespread naturally occurring mineral deposits and processed for use primarily in the construction industry. They are mined, crushed, sorted by size and sold loose or combined with portland cement or asphaltic cement to make concrete products to build roads, houses, buildings, and other structures. Much smaller quantities are used in agriculture, cement manufacture, chemical and metallurgical processes, glass production and many other products.

  5. Responsible construction?

    OpenAIRE

    Lou, Eric; Lee,Angela; WU, SONG; Mathison, Gill

    2015-01-01

    The ability of the construction industry to innovate in order to improve its practice has been widely debated over the years. As more and more organisations in other sectors, globally, are addressing 21st century consumer challenges: encompassing fair-trade, ethically sourced and more recycled products; and are reporting on their corporate responsibility performance (such as Marks and Spencer's Plan A, The Co-operative, The Body Shop etc), isn't it about time the construction industry followe...

  6. Metal-organic frameworks constructed from d-camphor acid: bifunctional properties related to luminescence sensing and liquid-phase separation.

    Science.gov (United States)

    Wen, Lili; Xu, Xiaoyue; Lv, Kangle; Huang, Yumei; Zheng, Xiaofang; Zhou, Li; Sun, Renqiang; Li, Dongfeng

    2015-02-25

    Three metal-organic frameworks (MOFs) [M2(d-cam)2(bimb)2]n · 3.5nH2O (M = Mn for 1, Co for 2) and [Cd8(d-cam)8(bimb)4]n (3) (d-H2cam = d-camphor acid, bimb = 4,4'-bis(1-imidazolyl)biphenyl), solvothermally synthesized, exhibit structural diversity. The charming aspect of these frameworks is that compound 3 is the very first MOF-based sensor for quantitatively detecting three different types of analytes (metal ions, aromatic molecules, and pesticides). And also, both compounds 2 and 3 show rapid uptake and ready regeneration for methyl orange (MO) and can selectively bind MO over methylene blue (MB) with high MO/MB separation ratio.

  7. Solvothermal Synthesis, Crystal Structure and Luminescent Property of a Dy(Ⅲ) Coordination Polymer Constructed by 1,2,4,5-Benzenetetracarboxylic Acid

    Institute of Scientific and Technical Information of China (English)

    BAI Wei-Wei; ZHU Ning; HAN Li-Min; tONG Hai-Long; GAOYuan-Yuan; SUO Quan-Ling

    2012-01-01

    A three-dimensional coordination polymer {[Dy(H2btec)2/4(btec)3/6(H2O)]·2H2O} n has been synthesized through the reaction of DyCl3·6H2O and 1,2,4,5-benzenetetracarboxylic acid by solvothermal technique, and the crystal structure was determined by X-ray diffraction. The Dy(Ⅲ) coordination polymer crystallizes in monoclinic, space group P21/n with a=10.7063(1), b=7.1491(1), c=17.1197(3), α=90, β=97.10, γ=90°, V=1300.31(3)3 , C10H9DyO11 , Dc=2.389g/cm3 , Z=4, F(000)=892, the final R=0.0165 and wR=0.0448 for I 〉 2σ(I). The title coordination polymer possesses a three-dimensional framework consisting of nine-coordinate Dy(Ⅲ) centers and two kinds of coordination modes for the 1,2,4,5-benzenetetracarboxylic acid ligands. Every btec4- ligand (deprotonated four protons from 1,2,4,5-benzenetetracarboxylic acid) in the title coordination polymer can clamp three Dy atoms like forceps, which could make the Dy atoms closer in the area of metal-metal interaction. The 3-D framework of the coordination polymer has many channels occupied by the free guest water molecules, and the hydrogen bonds between the coordinated carboxylic groups and guest water molecules could stabilize the crystal structure of the title coordination polymer. In addition, the luminescence properties were also studied.

  8. Construction and application of gold-labeled immunology assay for the detection of okadaic acid%软海绵酸胶体金免疫检测法的建立与应用

    Institute of Scientific and Technical Information of China (English)

    蔡春尔; 柳俊秀; 胡乐琴; 贾睿; 汪卿; 李春霞; 何培民

    2012-01-01

    Objective To construct and apply gold-labeled immunology assay for the detection of okadaic acid. Methods Gold-labeled immunology testing technology for fast detecting okadaic acid was established and optimized by a series of procedures,inclu-ding purification of monoclonal antibody of okadaic acid, preparation of colloidal gold, label of monoclonalantibody, preparation of strip,simulated sample extraction andspikedrecoverydetection,followed by extraction and testing of exposed shellfish. Results 30 nm colloidal gold particles were used to mark the test strip, the concentration and coated amount of go Id-labeled monoclonal anti-body were 200 μg/mL and 2. 5 μL/cm, respectively, and the concentration of second antibody was 1. 0 mg/mL. The colloidal gold test strip detection in spiked shellfish and exposed shellfish were applied, with detection time of 3 - 5 minutes and detection limit of less than 25 ng/mL. Conclusion Go Id-labeled immunology assay for fast detecting okadaic acid was established, meeting the safe threshold of okadaic acid in shellfish food ruled by many countries and proving technical foundation for detection of okadaic acid in shellfish.%目的 建立并应用软海绵酸(OA)胶体金免疫层析检测法.方法 通过纯化抗OA单抗、制备胶体金、标记单抗、制备胶体金试剂条、样品模拟提取与加标回收检测、贝类染毒提取及检测,建立和优化金标免疫层析法.结果 选择30 nm胶体金颗粒用于单抗标记,金标单抗浓度为200 μg/mL,金标单抗包被量为2.5 μL/cm,二抗包被浓度为1.0 mg/mL,反应时间为3~5 min,对加标贝肉和染毒贝类的检测灵敏度为25 ng/mL.结论 建立了可用于OA检测的胶体金免疫层析检测法,满足规定的贝类OA含量安全阈值,为腹泻性贝毒检测与食品安全检验提供了新的技术方法.

  9. Construction safety

    CERN Document Server

    Li, Rita Yi Man

    2013-01-01

    A close-to-ideal blend of suburb and city, speedy construction of towers of Babylon, the sparkling proportion of glass and steel buildings’ facade at night showcase the wisdom of humans. They also witness the footsteps, sweats and tears of architects and engineers. Unfortunately, these signatures of human civilizations are swathed in towering figures of construction accidents. Fretting about these on sites, different countries adopt different measures on sites. This book firstly sketches the construction accidents on sites, followed by a review on safety measures in some of the developing countries such as Bermuda, Egypt, Kuwait and China; as well as developed countries, for example, the United States, France and Singapore. It also highlights the enormous compensation costs with the courts’ experiences in the United Kingdom and Hong Kong.

  10. Activated Schwann Cell-Like Cells on Aligned Fibrin-Poly(Lactic-Co-Glycolic Acid) Structures: A Novel Construct for Application in Peripheral Nerve Regeneration.

    Science.gov (United States)

    Schuh, Christina M A P; Morton, Tatjana J; Banerjee, Asmita; Grasl, Christian; Schima, Heinrich; Schmidhammer, Robert; Redl, Heinz; Ruenzler, Dominik

    2015-01-01

    Tissue engineering approaches in nerve regeneration search for ways to support gold standard therapy (autologous nerve grafts) and to improve results by bridging nerve defects with different kinds of conduits. In this study, we describe electrospinning of aligned fibrin-poly(lactic-co-glycolic acid) (PLGA) fibers in an attempt to create a biomimicking tissue-like material seeded with Schwann cell-like cells (SCLs) in vitro for potential use as an in vivo scaffold. Rat adipose-derived stem cells (rASCs) were differentiated into SCLs and evaluated with flow cytometry concerning their differentiation and activation status [S100b, P75, myelin-associated glycoprotein (MAG), and protein 0 (P0)]. After receiving the proliferation stimulus forskolin, SCLs expressed S100b and P75; comparable to native, activated Schwann cells, while cultured without forskolin, cells switched to a promyelinating phenotype and expressed S100b, MAG, and P0. Human fibrinogen and thrombin, blended with PLGA, were electrospun and the alignment and homogeneity of the fibers were proven by scanning electron microscopy. Electrospun scaffolds were seeded with SCLs and the formation of Büngner-like structures in SCLs was evaluated with phalloidin/propidium iodide staining. Carrier fibrin gels containing rASCs acted as a self-shaping matrix to form a tubular structure. In this study, we could show that rASCs can be differentiated into activated, proliferating SCLs and that these cells react to minimal changes in stimulus, switching to a promyelinating phenotype. Aligned electrospun fibrin-PLGA fibers promoted the formation of Büngner-like structures in SCLs, which also rolled the fibrin-PLGA matrix into a tubular scaffold. These in vitro findings favor further in vivo testing.

  11. Nanostructuring effect of multi-walled carbon nanotubes on electrochemical properties of carbon foam as constructive electrode for lead acid battery

    Science.gov (United States)

    Kumar, Rajeev; Kumari, Saroj; Mathur, Rakesh B.; Dhakate, Sanjay R.

    2015-01-01

    In the present study, nanostructuring effect of multi-walled carbon nanotubes (MWCNTs) on electrochemical properties of coal tar pitch (CTP) based carbon foam (CFoam) was investigated. The different weight fractions of MWCNTs were mixed with CTP and foam was developed from the mixture of CTP and MWCNTs by sacrificial template technique and heat treated at 1,400 and 2,500 °C in inert atmosphere. These foams were characterized by scanning electron microscopy, X-ray diffraction, and potentiostat PARSTAT for cyclic voltammetry. It was observed that, bulk density of CFoam increases with increasing MWCNTs content and decreases after certain amount. The MWCNTs influence the morphology of CFoam and increase the width of ligaments as well as surface area. During the heat treatment, stresses exerting at MWCNTs/carbon interface accelerate ordering of the graphene layer which have positive effect on the electrochemical properties of CFoam. The current density increases from 475 to 675 mA/cm2 of 1,400 °C heat treated and 95 to 210 mA/cm2 of 2,500 °C heat-treated CFoam with 1 wt% MWCNTs. The specific capacitance was decreases with increasing the scan rate from 100 to 1,000 mV/s. In case of 1 % MWCNTs content CFoam the specific capacitance at the scan rate 100 mV/s was increased from 850 to 1,250 μF/cm2 and 48 to 340 μF/cm2 of CFoam heat treated at 1,400 °C and 2,500 °C respectively. Thus, the higher value surface area and current density of MWCNTs-incorporated CFoam heat treated to 1,400 °C can be suitable for lead acid battery electrode with improved charging capability.

  12. Construction work

    CERN Multimedia

    2004-01-01

    Construction work on building 179 will start on the 16th February 2004 and continue until November 2004. The road between buildings 179 and 158 will temporarily become a one way street from Route Democrite towards building 7. The parking places between buildings 179 and 7 will become obsolete. The ISOLDE collaboration would like to apologize for any inconveniences.

  13. Layout Construction

    DEFF Research Database (Denmark)

    Frandsen, Gudmund Skovbjerg; Palsberg, Jens; Schmidt, Erik Meineche

    We design a system for generating newspaper layout proposals. The input to the system consists of editorial information (text, pictures, etc) and style information (non-editorial information that specifies the aesthetic appearance of a layout). We consider the automation of layout construction...

  14. Sandwich construction

    Science.gov (United States)

    Marshall, A.

    A form of composites known as structural sandwich construction is presented in terms of materials used, design details for solving edging and attachment problems, and charts of design material analysis. Sandwich construction is used in nearly all commercial airliners and helicopters, and military air and space vehicles, and it is shown that this method can stiffen a structure without causing a weight increase. The facing material can be made of 2024 or 7075 aluminum alloy, titanium, or stainless steel, and the core material can be wood or foam. The properties of paper honeycomb and various aluminum alloy honeycombs are presented. Factors pertaining to adhesive materials are discussed, including products given off during cure, bonding pressure, and adaptability. Design requirements and manufacturing specifications are resolved using numerous suggestions.

  15. pH非敏感型自交联共轭亚油酸囊泡的构建和表征%Construction and Characterization of a Novel pH-insensitive Self-crosslinked Ufasome of Conjugated Linoleic Acid

    Institute of Scientific and Technical Information of China (English)

    樊晔; 方云; 高迪

    2014-01-01

    Conjugated linoleic acid( CLA) was employed as a molecular building block to construct a novel pH-insensitive fatty acid vesicle(FAV). First, CLA was semi-synthesized from linoleic acid through alkali isomerization. Subsequently, the ufasome(unsaturated fatty acid liposome) of CLA was self-assembled from the CLA molecules together with the CLA ions in response to pH, and a novel pH-insensitive FAV, the self-crosslinked-CLA FAV, was prepared by chemical tethering the CLA-ufasome through UV irradiation. The sui-table pH ranges and the CLA concentrations were determined by acid-base titration and surface tension mea-surement. The morphology of the self-crosslinked-CLA FAV was imaged by transmission electro microscopy ( TEM) and the pH-insensitive characteristic of the self-crosslinked-CLA FAV was tested by dynamic light scattering ( DLS ) . The experimental results indicated that the self-crosslinked-CLA FAV with diameter of 10-20 nm and wall thickness of 2.0 nm was obtained through a two-step procedure. At first, the CLA-ufa-some formed using the CLA species as a molecular building block at pH=8.6 and CLA concentration of 3 mmol/L, and then followed by UV irradiation self-crosslinking for 2.5 h. It was verified by DLS data that the self-crosslinked-CLA FAV is pH-insensitive in almost all pH ranges. Finally, the in vitro release results of 5-fluorouracil from the self-crosslinked-CLA FAV showed that the process was slow and sustainable.%以共轭亚油酸( Conjugated linoleic acid, CLA)为构造pH非敏感型脂肪酸囊泡( Fatty acid vesicle, FAV)的分子砌块,通过碱异构化法从亚油酸半合成CLA,然后采用pH刺激响应自组装法获得CLA的不饱和脂肪酸囊泡( Ufasome),采用紫外辐照诱导方式对CLA-ufasome实施囊泡内化学绑定,获得一种新的pH非敏感型FAV.通过酸碱滴定和表面张力实验确定CLA-ufasome形成的适宜pH范围和浓度,利用透射电子显微镜( TEM)表征了自交联CLA的FAV的

  16. A series of coordination polymers constructed from R-isophthalic acid (R=-SO3H, -NO2, and -OH) and N-donor ligands: Syntheses, structures and fluorescence properties

    Science.gov (United States)

    Zhou, Yong-Hong; Zhou, Xu-Wan; Zhou, Su-Rong; Tian, Yu-Peng; Wu, Jie-Ying

    2017-01-01

    Six novel Zn(II), Cd(II), and Cu(II) mixed-ligand coordination complexes, namely, [Zn2Na(sip)2(bpp)3(Hbpp)(H2O)2]·8H2O (1), [Cd3(sip)2(nbi)6(H2O)2]·7H2O (2), [Zn(sip)(nbi)2(H2O)]·Hnbi·3H2O (3), [Cd(hip)(nbi)2(H2O)]·nbi·5H2O (4), [Cd2(nip)2(nbi)2(H2O)2]·DMF (5), and [Cu(nip)(nbi)(H2O)2]·H2O (6) (H3sip=5-sulfoisophthalic acid, H2hip=5-hydroxylisophthalic acid, H2nip=5-nitroisophthalic acid, bpp=1,3-bis(4-pyridyl)propane, and nbi=6-nitrobenzimidazole) have been synthesized hydrothermally by the self-assembly of R-isophthalic acid (R=-SO3H, -NO2, and -OH) and N-donor ligands. Single crystal X-ray analyses reveal that two Zn(II) ions and one Na(I) ion of complex 1 are linked through O atoms to generate a 1D linear chain. Then the 2D supramolecular architectures are constructed via intermolecular interactions. In complex 2, the Cd1 ions are connected by bridging carboxyl groups from sip3- anions to form 1D double chain, which are further connected by Cd2 ions to afford 2D layer structure. The adjacent 2D layers are further linked via hydrogen-bonding interactions to give 3D supramolecular network. Compounds 3-5 show 1D chain structures, which are assembled into 2D or 3D supramolecular frameworks via weak interactions. In compound 6, the Cu(II) ions are bridged by the nip2- ligands to form 48-membered ring, which is assembled into 1Dchain via the π-π stacking interaction. In addition, the thermal stabilities and fluorescence properties of these compounds have also been studied.

  17. Constructing Catalonia

    Directory of Open Access Journals (Sweden)

    Bill Philips

    2009-07-01

    Full Text Available Catalonia, in common with other nations, has long been concerned with the question of identity and difference. Its problematic relationship with Spain has led to an emphasis on differentiating itself from its larger neighbour (if we are to accept, as most Spaniards do not, that Catalonia is not Spain, a situation complicated by the loss of the Spanish colonies of Cuba and The Philippines in 1898, and the Spanish Civil War and subsequent dictatorship from 1936 to 1976. Beginning in the late nineteenth century, the construction of a Catalan identity followed a similar route to that taken by other European nations such as England, Ireland and, indeed, Spain, including an emphasis on rural values, activities and the countryside, and the conversion of specifically local traditions into national past times. It is only in the last ten years or so that this model of Catalan identity has been recognised for what it is – a model constructed and encouraged for and by specific nationalist political interests. Ironically, Catalonia’s identity abroad has also been constructed and manipulated for political purposes, but from quite a different perspective. Orwell’s /Homage to Catalonia/ (1938 narrates an extremely blinkered version of the Spanish Civil War which has achieved iconic status as a result of cold war politics. Subsequent portrayals of the Spanish Civil War – Valentine Cunningham’s /The Penguin Book of Spanish Civil War Verse/ (ed., Penguin, 1980, or Ken Loach’s 1995 film /Land and Freedom/ base their arguments unquestioningly on /Homage to Catalonia/, perpetuating a view of the nation’s recent history that is both reductive and inaccurate

  18. Constructing History

    DEFF Research Database (Denmark)

    Jørgensen, Michael Riber

    2010-01-01

    The Icelandic sagas can be read and interpreted in many ways. This article examines the sagas both as literary expressions of a longstanding oral tradition and as part of a collective and cultural memory. The focus in the first part is on people and places in the sagas as ‘realms of memory’: thin...... that help construct a common past and a common identity. The second part of the article explores the role of the sagas in medieval Iceland as ‘key myths’ that explain the origin and uniqueness of a society, and as moral and legal role models legitimizing the current social order....

  19. Construction material

    Science.gov (United States)

    Wagh, Arun S.; Antink, Allison L.

    2008-07-22

    A structural material of a polystyrene base and the reaction product of the polystyrene base and a solid phosphate ceramic is applied as a slurry which includes one or more of a metal oxide or a metal hydroxide with a source of phosphate to produce a phosphate ceramic and a poly (acrylic acid or acrylate) or combinations or salts thereof and polystyrene or MgO applied to the polystyrene base and allowed to cure so that the dried aqueous slurry chemically bonds to the polystyrene base. A method is also disclosed of applying the slurry to the polystyrene base.

  20. Constructing Knowledge

    Science.gov (United States)

    Blanton, Patricia

    2003-02-01

    Schools are expected to lay the foundation upon which knowledge can be built and equip students with the tools necessary to accomplish the construction. The role of the teacher in this building process is crucial to the type of structure the student can build. Whether you call it constructivism, discussion teaching, project-based learning, inquiry learning, or any of the other names given to the instructional strategies being suggested by education researchers, the key is getting students to become active participants in the process. While some students may be able to learn from eloquently delivered lectures and dynamic demonstrations, the majority of students cannot effectively retain and apply ideas communicated in this manner.

  1. Aspartic acid

    Science.gov (United States)

    Aspartic acid is a nonessential amino acids . Amino acids are building blocks of proteins. "Nonessential" means that our ... this amino acid from the food we eat. Aspartic acid is also called asparaginic acid. Aspartic acid helps ...

  2. Plasmid construction using recombination activity in the fission yeast Schizosaccharomyces pombe.

    Directory of Open Access Journals (Sweden)

    Ayako Chino

    Full Text Available BACKGROUND: Construction of plasmids is crucial in modern genetic manipulation. As of now, the common method for constructing plasmids is to digest specific DNA sequences with restriction enzymes and to ligate the resulting DNA fragments with DNA ligase. Another potent method to construct plasmids, known as gap-repair cloning (GRC, is commonly used in the budding yeast Saccharomyces cerevisiae. GRC makes use of the homologous recombination activity that occurs within the yeast cells. Due to its flexible design and efficiency, GRC has been frequently used for constructing plasmids with complex structures as well as genome-wide plasmid collections. Although there have been reports indicating GRC feasibility in the fission yeast Schizosaccharomyces pombe, this species is not commonly used for GRC as systematic studies of reporting GRC efficiency in S. pombe have not been performed till date. METHODOLOGY/PRINCIPAL FINDINGS: We investigated GRC efficiency in S. pombe in this study. We first showed that GRC was feasible in S. pombe by constructing a plasmid that contained the LEU2 auxotrophic marker gene in vivo and showed sufficient efficiency with short homology sequences (>25 bp. No preference was shown for the sequence length from the cut site in the vector plasmid. We next showed that plasmids could be constructed in a proper way using 3 DNA fragments with 70% efficiency without any specific selections being made. The GRC efficiency with 3 DNA fragments was dramatically increased >95% in lig4Delta mutant cell, where non-homologous end joining is deficient. Following this approach, we successfully constructed plasmid vectors with leu1+, ade6+, his5+, and lys1+ markers with the low-copy stable plasmid pDblet as a backbone by applying GRC in S. pombe. CONCLUSIONS/SIGNIFICANCE: We concluded that GRC was sufficiently feasible in S. pombe for genome-wide gene functional analysis as well as for regular plasmid construction. Plasmids with different

  3. 3-PBA降解菌BA3的降解特性及基因工程菌构建%Characteristics of a 3-Phenoxybenzoic Acid Degrading-Dacterium and the Construction of a Engineering Bacterium

    Institute of Scientific and Technical Information of China (English)

    段晓芹; 郑金伟; 张隽; 杭宝建; 何健; 李顺鹏

    2011-01-01

    从石油污染土壤中分离到1株能以3-苯氧基苯甲酸(3-PBA)为唯一碳源生长的细菌,命名为BA3.根据其生理生化特征和16S rDNA序列相似性分析,将该菌鉴定为鞘脂菌属(Sphingobium sp.).该菌株在60 h内对100 mg·L-1的3-苯氧基苯甲酸的降解率达到99%.降解3-苯氧基苯甲酸的最适温度为30℃,pH值为7.0,降解速率与初始接种量呈正相关.拟除虫菊酯类杀虫剂水解酶基因pytH为本实验室克隆到的一个新的菊酯水解酶基因,通过PCR从菊酯降解菌株JZ-2总DNA扩增了pytH,将pytH定向克隆到pBBRMCS-5载体上,构建重组质粒pPYTH,在辅助质粒pBK600的帮助下,通过三亲接合将pPYTH转移到3-PBA降解菌株Sphingobium sp.BA3中,获得工程菌Sphingobium sp.BA3-pytH;甲氰菊酯的降解实验表明,菌株JZ-2在48 h内对50 mg·L-1的甲氰菊酯降解率仅为60%左右,工程菌BA3一pytH相同条件下对50 mg·L-1的甲氰菊酯降解率达到95%以上,同时对甲氰菊酯的降解中间产物3-PBA也能较快降解,不会对菊酯的降解产生反馈抑制.工程菌BA3-pytH因其对甲氰菊酯和其降解中间产物3-PBA的快速降解能力,比原始菌株JZ-2在菊酯污染土壤生物修复应用中更具有优势.%A bacterium capable of utilizing 3-phenoxybenzoic acid (3-FBA) as sole carbon source was isolated trom petroleumcontaminated soil. This bacterium, designated as BA3, was identified as Sphingobium sp. according to its physiological & biochemical characteristic and the similarity analysis of its 16S rDNA sequence. Strain BA3 was able to degrade 99% of 100 mg· L-1 3-phenoxybenzoic acid within 60 h. The optimal pH and temperature for the degradation were 7.0 and 30℃, respectively. The degradation efficiency was related positively to initial inoculum size. The pyrethroid hydrolase gene(pytH) gene was amplified from the genomic DNA of Sphingobium sp. JZ-2 by PCR. Recombinant plasmids pPYTH was constructed by ligating pytH gene into the broad

  4. Concrete construction engineering handbook

    CERN Document Server

    Nawy, Edward G

    2008-01-01

    Provides coverage of concrete construction engineering and technology. This work features discussions focusing on: the advances in engineered concrete materials; reinforced concrete construction; specialized construction techniques; and, design recommendations for high performance.

  5. Polymer supported sulphanilic acid: A highly efficient and recyclable green heterogeneous catalyst for the construction of 4,5-dihydropyrano[3,2-c]chromenes under solvent-free conditions

    Indian Academy of Sciences (India)

    Jashmin P Patel; Jemin R Avalani; Dipak K Raval

    2013-05-01

    Polystyrene divinylbenzene supported sulphanilic acid was prepared, characterized and tested as an acidic catalyst in multicomponent organic synthesis. This is the first report focusing on the development of one-pot solvent-free synthesis of 4,5- dihydropyrano [3,2-c]chromenes under acidic conditions. It proved to be a versatile catalyst for microwave-assisted synthesis of 4,5-dihydropyrano[3,2-c]chromenes. This methodology is mild, high yielding, green and the catalyst could be easily recycled for several times.

  6. Constructing an Archive

    DEFF Research Database (Denmark)

    Carbone, Claudia; Wieczorek, Izabela; Francis, Alice;

    2016-01-01

    "Constructing an Archive" contains a collection of student works conducted during the Fall semester of 2015 at the Aarhus School of Architecture, at the master studio Constructing an Archive......."Constructing an Archive" contains a collection of student works conducted during the Fall semester of 2015 at the Aarhus School of Architecture, at the master studio Constructing an Archive....

  7. Determination of acute lethal and chronic lethal dose thresholds of valproic acid using 3D spheroids constructed from the immortal human hepatocyte cell line HepG2/C3A

    DEFF Research Database (Denmark)

    Fey, S. J.; Wrzesinski, K.

    2013-01-01

    Valproic acid (VPA) is a broad-spectrum antiepileptic drug that is now used commonly for several other neurological and psychiatric indications. While VPA is usually well tolerated, on rare occasions, it has been associated with severe, and sometimes, fatal liver injuries. These complications may...... describe here a culture system based on 3D spheroid culture of immortal hepatocytes which can determine the toxicity of valproic acid (or structurally or functionally related molecules) in vitro. The spheroids were used to follow changes in ATP production, glucose uptake and adenylate kinase following...... also arise due to acute VPA overdose. As a branched chain carboxylic acid, VPA is readily metabolized in the liver via glucuronic acid conjugation, mitochondrial β- and cytosolic ω-oxidation to produce multiple metabolites, and it is probably some of these metabolites that are involved in its toxicity...

  8. Construction of modular tandem expression vectors for the green alga Chlamydomonas reinhardtii using the Cre/lox-system.

    Science.gov (United States)

    Heitzer, Markus; Zschoernig, Barbara

    2007-09-01

    The successful expression of foreign genes mainly depends on both a reliable method for transformation and a suitable promoter sequence. We created a series of modular plasmids that facilitate the rapid construction of large tandem vectors for transgene expression under the control of different promoter sequences in Chlamydomonas reinhardtii. Tandem vectors carrying expression cassettes for Renilla luciferase and a metabolic selection marker (ARG7) were manufactured by fusing two plasmids in vitro using Cre/lox site-specific recombination. Supercoiled and linear plasmids were used to transform an arginine auxotrophic Chlamydomonas strain, and rates of co-expression as well as levels of luciferase activity were monitored for frequently used promoters (HSP70A, LHCB1, PSAD, and the chimeric HSP70A/RBCS2). Linearized tandem vectors generally increased the co-expression frequency (up to 77%) compared with standard cotransformation protocols. Most transformants showed a single and complete integration event confirming the close linkage of active selectable marker and reporter gene within the nuclear genome. The analysis of luciferase activity showed expression levels within three orders of magnitude for the promoters used, with the artificial HSP70A/RRBCS2 being the most active. For 69% of all luminescent transformants carrying the HSP70A promoter luciferase expression was enhanced by heatshock, indicating physiological promoter function in a transgenic context.

  9. The Role of Amino Acid Permeases and Tryptophan Biosynthesis in Cryptococcus neoformans Survival.

    Directory of Open Access Journals (Sweden)

    João Daniel Santos Fernandes

    Full Text Available Metabolic diversity is an important factor during microbial adaptation to different environments. Among metabolic processes, amino acid biosynthesis has been demonstrated to be relevant for survival for many microbial pathogens, whereas the association between pathogenesis and amino acid uptake and recycling are less well-established. Cryptococcus neoformans is an opportunistic fungal pathogen with many habitats. As a result, it faces frequent metabolic shifts and challenges during its life cycle. Here we studied the C. neoformans tryptophan biosynthetic pathway and found that the pathway is essential. RNAi indicated that interruptions in the biosynthetic pathway render strains inviable. However, auxotroph complementation can be partially achieved by tryptophan uptake when a non preferred nitrogen source and lower growth temperature are applied, suggesting that amino acid permeases may be the target of nitrogen catabolism repression (NCR. We used bioinformatics to search for amino acid permeases in the C. neoformans and found eight potential global permeases (AAP1 to AAP8. The transcriptional profile of them revealed that they are subjected to regulatory mechanisms which are known to respond to nutritional status in other fungi, such as (i quality of nitrogen (Nitrogen Catabolism Repression, NCR and carbon sources (Carbon Catabolism Repression, CCR, (ii amino acid availability in the extracellular environment (SPS-sensing and (iii nutritional deprivation (Global Amino Acid Control, GAAC. This study shows that C. neoformans has fewer amino acid permeases than other model yeasts, and that these proteins may be subjected to complex regulatory mechanisms. Our data suggest that the C. neoformans tryptophan biosynthetic pathway is an excellent pharmacological target. Furthermore, inhibitors of this pathway cause Cryptococcus growth arrest in vitro.

  10. USAID Construction Assessment

    Data.gov (United States)

    US Agency for International Development — The USAID construction assessment is a survey of the character, scope, value and management of construction activities supported by USAID during the period from...

  11. Professional entrepreneurial identity construction

    DEFF Research Database (Denmark)

    Ramsgaard, Michael Breum

    2014-01-01

    The present study investigates the construction of a professional identity as an entrepreneur in a sample of people with educational background in nutrition and health. The study examines the connection between professional identity construction and entrepreneurial business emergence using...

  12. Organizational Behaviour in Construction

    DEFF Research Database (Denmark)

    Kreiner, Kristian

    2013-01-01

    Review of: Organizational Behaviour in Construction / Anthony Walker (Wiley-Blackwell,2011 336 pp)......Review of: Organizational Behaviour in Construction / Anthony Walker (Wiley-Blackwell,2011 336 pp)...

  13. Validating MEDIQUAL Constructs

    Science.gov (United States)

    Lee, Sang-Gun; Min, Jae H.

    In this paper, we validate MEDIQUAL constructs through the different media users in help desk service. In previous research, only two end-users' constructs were used: assurance and responsiveness. In this paper, we extend MEDIQUAL constructs to include reliability, empathy, assurance, tangibles, and responsiveness, which are based on the SERVQUAL theory. The results suggest that: 1) five MEDIQUAL constructs are validated through the factor analysis. That is, importance of the constructs have relatively high correlations between measures of the same construct using different methods and low correlations between measures of the constructs that are expected to differ; and 2) five MEDIQUAL constructs are statistically significant on media users' satisfaction in help desk service by regression analysis.

  14. Personality Constructs and Measures

    Science.gov (United States)

    Teglasi, Hedwig; Simcox, April G.; Kim, Na-Young

    2007-01-01

    A psychological construct, such as personality, is an abstraction that is not directly seen but inferred through observed regularities in cognitive, affective, and behavioral responses in various settings. Two assumptions give meaning to the idea of construct validity. First, constructs represent real phenomena that exist apart from the potential…

  15. Component School Construction Program.

    Science.gov (United States)

    New Brunswick Dept. of Economic Growth, Fredericton.

    In 1968, the Province of New Brunswick initiated a three-phase program to provide for elementary school facilities, employing a component systems approach to their construction. This booklet describes briefly the planning and construction of these schools, and provides graphic and photographic records of the construction in progress as well as of…

  16. "Task" as Research Construct

    Science.gov (United States)

    Seedhouse, Paul

    2005-01-01

    The article examines "task" as research construct as predominantly conceived in terms of task-as-workplan in the task-based learning/second language acquisition literature. It is suggested that "task" has weak construct validity and ontology in an overwhelmingly quantitative paradigm because the construct has a "split personality."…

  17. A Duplicate Construction Experiment.

    Science.gov (United States)

    Bridgeman, Brent

    This experiment was designed to assess the ability of item writers to construct truly parallel tests based on a "duplicate-construction experiment" in which Cronbach argues that if the universe description and sampling are ideally refined, the two independently constructed tests will be entirely equivalent, and that within the limits of item…

  18. Engineering biotin prototrophic Corynebacterium glutamicum strains for amino acid, diamine and carotenoid production.

    Science.gov (United States)

    Peters-Wendisch, P; Götker, S; Heider, S A E; Komati Reddy, G; Nguyen, A Q; Stansen, K C; Wendisch, V F

    2014-12-20

    The Gram-positive Corynebacterium glutamicum is auxotrophic for biotin. Besides the biotin uptake system BioYMN and the transcriptional regulator BioQ, this bacterium possesses functional enzymes for the last three reactions of biotin synthesis starting from pimeloyl-CoA. Heterologous expression of bioF from the Gram-negative Escherichia coli enabled biotin synthesis from pimelic acid added to the medium, but expression of bioF together with bioC and bioH from E. coli did not entail biotin prototrophy. Heterologous expression of bioWAFDBI from Bacillus subtilis encoding another biotin synthesis pathway in C. glutamicum allowed for growth in biotin-depleted media. Stable growth of the recombinant was observed without biotin addition for eight transfers to biotin-depleted medium while the empty vector control stopped growth after the first transfer. Expression of bioWAFDBI from B. subtilis in C. glutamicum strains overproducing the amino acids l-lysine and l-arginine, the diamine putrescine, and the carotenoid lycopene, respectively, enabled formation of these products under biotin-depleted conditions. Thus, biotin-prototrophic growth and production by recombinant C. glutamicum were achieved.

  19. Lactic acid bacteria producing B-group vitamins: a great potential for functional cereals products.

    Science.gov (United States)

    Capozzi, Vittorio; Russo, Pasquale; Dueñas, María Teresa; López, Paloma; Spano, Giuseppe

    2012-12-01

    Wheat contains various essential nutrients including the B group of vitamins. However, B group vitamins, normally present in cereals-derived products, are easily removed or destroyed during milling, food processing or cooking. Lactic acid bacteria (LAB) are widely used as starter cultures for the fermentation of a large variety of foods and can improve the safety, shelf life, nutritional value, flavor and overall quality of the fermented products. In this regard, the identification and application of strains delivering health-promoting compounds is a fascinating field. Besides their key role in food fermentations, several LAB found in the gastrointestinal tract of humans and animals are commercially used as probiotics and possess generally recognized as safe status. LAB are usually auxotrophic for several vitamins although certain strains of LAB have the capability to synthesize water-soluble vitamins such as those included in the B group. In recent years, a number of biotechnological processes have been explored to perform a more economical and sustainable vitamin production than that obtained via chemical synthesis. This review article will briefly report the current knowledge on lactic acid bacteria synthesis of vitamins B2, B11 and B12 and the potential strategies to increase B-group vitamin content in cereals-based products, where vitamins-producing LAB have been leading to the elaboration of novel fermented functional foods. In addition, the use of genetic strategies to increase vitamin production or to create novel vitamin-producing strains will be also discussed.

  20. Amoeba host-Legionella synchronization of amino acid auxotrophy and its role in bacterial adaptation and pathogenic evolution.

    Science.gov (United States)

    Price, Christopher T D; Richards, Ashley M; Von Dwingelo, Juanita E; Samara, Hala A; Abu Kwaik, Yousef

    2014-02-01

    Legionella pneumophila, the causative agent of Legionnaires' disease, invades and proliferates within a diverse range of free-living amoeba in the environment, but upon transmission to humans, the bacteria hijack alveolar macrophages. Intracellular proliferation of L. pneumophila in two evolutionarily distant hosts is facilitated by bacterial exploitation of conserved host processes that are targeted by bacterial protein effectors injected into the host cell. A key aspect of microbe-host interaction is microbial extraction of nutrients from the host, but understanding of this is still limited. AnkB functions as a nutritional virulence factor and promotes host proteasomal degradation of polyubiquitinated proteins generating gratuitous levels of limiting host cellular amino acids. Legionella pneumophila is auxotrophic for several amino acids including cysteine, which is a metabolically preferred source of carbon and energy during intracellular proliferation, but is limiting in both amoebae and humans. We propose that synchronization of bacterial amino acids auxotrophy with the host is a driving force in pathogenic evolution and nutritional adaptation of L. pneumophila and other intracellular bacteria to life within the host cell. Understanding microbial strategies of nutrient generation and acquisition in the host will provide novel antimicrobial strategies to disrupt pathogen access to essential sources of carbon and energy.

  1. Lunar base construction requirements

    Science.gov (United States)

    Jolly, Steve; Helleckson, Brent

    1990-01-01

    The following viewgraph presentation is a review of the Lunar Base Constructibility Study carried out in the spring and summer of 1990. The objective of the study was to develop a method for evaluating the constructibility of Phase A proposals to build facilities on orbit or on extraterrestrial surfaces. Space construction was broadly defined as all forms of assembly, disassembly, connection, disconnection, deployment, stowage, excavation, emplacement, activation, test, transportation, etc., required to create facilities in orbit and on the surfaces of other celestial bodies. It was discovered that decisions made in the face of stated and unstated assumptions early in the design process (commonly called Phase A) can lock in non-optimal construction methods. Often, in order to construct the design, alterations must be made to the design during much later phases of the project. Such 'fixes' can be very difficult, expensive, or perhaps impossible. Assessing constructibility should thus be a part of the iterative design process, starting with the Phase A studies and continuing through production. This study assumes that there exists a minimum set of key construction requirements (i.e., questions whose answers form the set of discriminators) that must be implied or specified in order to assess the constructibility of the design. This set of construction requirements constitutes a 'constructibility filter' which then becomes part of the iterative design process. Five inherently different, dichotomous design reference missions were used in the extraction of these requirements to assure the depth and breath of the list.

  2. Folic acid

    Science.gov (United States)

    ... taking folic acid by itself, or with L-carnitine a compound similar to an amino acid from ... levels. It is not clear if folic acid supplementation reduces hearing loss in people with normal folate ...

  3. Kirchnerist construction of memory

    OpenAIRE

    Bermúdez, Nicolás

    2015-01-01

    The effects on the construction of collective memory caused by the arrival of the kirchnerism to the political field have been extensively studied. That construction suffered transformations with the change of president, which have been less studied. Therefore, the objective of this paper is to present an analysis about the discursive construction of memory during Cristina Fernández de Kirchner first government. This research is part of the discourse studies, and more precisely it is part of ...

  4. Constructing Student Problems in Phylogenetic Tree Construction.

    Science.gov (United States)

    Brewer, Steven D.

    Evolution is often equated with natural selection and is taught from a primarily functional perspective while comparative and historical approaches, which are critical for developing an appreciation of the power of evolutionary theory, are often neglected. This report describes a study of expert problem-solving in phylogenetic tree construction.…

  5. Xinjiang Dushanzi Project Started Construction Started Construction

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ Xinjiang Dushanzi petroleum refining and chemical integrated project,is the largest one so far in China, with a capacity of ten-million-ton petroleum refinery and onemillion-ton ethylene complex, directly under PetroChina Company Limited (PetroChina), was started construction along with laying a foundation on August 22, 2005.

  6. Construction Technology Transfer Center

    Science.gov (United States)

    1998-12-01

    44 USACERL TR 99/11 References Arditi , D., "Construction Productivity Improvement," Journal of Construction Engi- neering and Management, American...8. 12 Ichinowski, T. and Setzer, S. "Corps seeks plans to boost productivity." Engineering News Record, February 9, 1989, p. 11-12. 13 Arditi , D

  7. Human Resource Construction

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    Centering on strategic objective of reform and development,CIAE formulated its objectives in human resource construction for the 13th Five-year Plan period,and achieved new apparent progress in human resource construction in 2015.1 Implementation of"LONGMA Project"

  8. Personal Usability Constructs

    DEFF Research Database (Denmark)

    Hertzum, Morten; Clemmensen, Torkil; Hornbæk, Kasper

    2011-01-01

    Whereas the concept of usability is predominantly defined analytically, people relate to systems through personal usability constructs. Based on 48 repertory-grid interviews, this study investigates how such personal constructs are affected by two factors crucial to the international development ...

  9. Acquisition of Comparison Constructions

    Science.gov (United States)

    Hohaus, Vera; Tiemann, Sonja; Beck, Sigrid

    2014-01-01

    This article presents a study on the time course of the acquisition of comparison constructions. The order in which comparison constructions (comparatives, measure phrases, superlatives, degree questions, etc.) show up in English- and German-learning children's spontaneous speech is quite fixed. It is shown to be insufficiently determined by…

  10. Railway Construction in Tibet

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Authored by Li Ping and Li Xinhu, the book actually is the result of research into “schemes of construction of railways in Yunnan and Tibet.” The book centers around research into the scheme of construction of the YunnanTibet Railway, conducting comprehensive studies involving political, economic, technological, social and State security fields.

  11. Motivating Workers in Construction

    Directory of Open Access Journals (Sweden)

    Jason E. Barg

    2014-01-01

    Full Text Available The study of the motivation of construction workers is limited to a relatively small body of knowledge. Although there is considerable research available regarding motivation and productivity, few researchers have provided a comprehensive analysis on the motivation of construction workers. The research stated that productivity in construction has not improved compared to other industry sectors such as manufacturing. This trend has been echoed in publications throughout the past five decades, and suggested that motivation is one of the key factors impacting productivity. This paper offers a comprehensive review of the published work that directly links the key words—construction and motivation. The findings have been presented in five themes, that is, motivation models, environment and culture, incentives and empowerment, and worker management. This paper concludes with two methods suggested by previous researchers to improve motivation of construction workers: (1 relevant worker incentives (intrinsic or extrinsic and (2 improved management practices, specifically regarding communication with workers.

  12. Operations management for construction

    CERN Document Server

    March, Chris

    2009-01-01

    Students studying construction management and related subjects need to have a broad understanding of the major aspects of controlling the building processes. Operations Management for Construction is one of three textbooks (Business Organisation, Operations Management and Finance Control) written to systematically cover the field. Focusing on construction sites and operations which are challenging to run, Chris March explores issues such as the setting up of the site, the deciding of the methodology of construction, and the sequence of work and resourcing. As changing and increasing regulations affect the way sites are managed, he also considers the issues and methods of successful administering, safety, quality and environment. Finally, the contractor's responsibility to the environment, including relationships with third parties, selection of materials, waste management and sustainability is discussed. Chris March has a wealth of practical experience in the construction industry, as well as considerable exp...

  13. VENTILATION NEEDS DURING CONSTRUCTION

    Energy Technology Data Exchange (ETDEWEB)

    C.R. Gorrell

    1998-07-23

    The purpose of this analysis is to determine ventilation needs during construction and development of the subsurface repository and develop systems to satisfy those needs. For this analysis, construction is defined as pre-emplacement excavation and development is excavation that takes place simultaneously with emplacement. The three options presented in the ''Overall Development and Emplacement Ventilation Systems'' analysis (Reference 5.5) for development ventilation will be applied to construction ventilation in this analysis as well as adding new and updated ventilation factors to each option for both construction and development. The objective of this analysis is to develop a preferred ventilation system to support License Application Design. The scope of this analysis includes: (1) Description of ventilation conditions; (2) Ventilation factors (fire hazards, dust control, construction logistics, and monitoring and control systems); (3) Local ventilation alternatives; (4) Global ventilation options; and (5) Evaluation of options.

  14. Governmentalities of Construction

    DEFF Research Database (Denmark)

    Gottlieb, Stefan; Jensen, Jens Stissing

    2016-01-01

    In this paper, we apply Foucault’s concept of governmentality in a dual analysis of the formation and transformation of the construction sector and the construction worker. The governmentality concept is well-suited for such an analysis as it directs attention to the ways in which control...... is the exercised over a specific area of institutional life through the shaping of individuals’ conduct. We argue that construction, as a coherent sector, first was rendered governable in the 1940s in order to achieve national modernisation. It is shown how the political measures that were based on the exercise...... of disciplinary power also impacted the formation of identities constituting the construction worker as a normalised subject. We then illustrate how construction since the mid-1990s has been shaped by two contrasting governmentalities framing the sector as respectively a resource area, with emphasis on innovation...

  15. Gluconic acid production.

    Science.gov (United States)

    Anastassiadis, Savas; Morgunov, Igor G

    2007-01-01

    Gluconic acid, the oxidation product of glucose, is a mild neither caustic nor corrosive, non toxic and readily biodegradable organic acid of great interest for many applications. As a multifunctional carbonic acid belonging to the bulk chemicals and due to its physiological and chemical characteristics, gluconic acid itself, its salts (e.g. alkali metal salts, in especially sodium gluconate) and the gluconolactone form have found extensively versatile uses in the chemical, pharmaceutical, food, construction and other industries. Present review article presents the comprehensive information of patent bibliography for the production of gluconic acid and compares the advantages and disadvantages of known processes. Numerous manufacturing processes are described in the international bibliography and patent literature of the last 100 years for the production of gluconic acid from glucose, including chemical and electrochemical catalysis, enzymatic biocatalysis by free or immobilized enzymes in specialized enzyme bioreactors as well as discontinuous and continuous fermentation processes using free growing or immobilized cells of various microorganisms, including bacteria, yeast-like fungi and fungi. Alternatively, new superior fermentation processes have been developed and extensively described for the continuous and discontinuous production of gluconic acid by isolated strains of yeast-like mold Aureobasidium pullulans, offering numerous advantages over the traditional discontinuous fungi processes.

  16. Synthesis and Crystal Structure of a Novel 2D Cadmium(Ⅱ) Complex Constructed from N-(3-Carboxyphenyl)-4,4'-bipyridinium and Benzene-1,4-dicarboxylic Acid

    Institute of Scientific and Technical Information of China (English)

    WU Wei; JIN Xu-Hui; JU Zhang-Feng; ZHANG Jie

    2008-01-01

    A novel redox-active bipyridinium ligand N-(3-carboxyphenyl)-4,4'-bipyridinium chloride (HCPBPyCl) and its coordination polymer {[Cd2(CPBPy)2(BDC)(H2O)2Cl2]·6H2O}n 1 (H2BDC = benzene-1,4-dicarboxylic acid) were synthesized and characterized. Crystallographic data for 1: C42H44Cd2C12N4O16, Mr = 1156.51, monoclinic, space group C2/c, a = 21.046(3), b = 11.0036(15), c = 20.012(3)A, β = 98.694(8)°, Z = 4, V = 4581.1(11) A3, Dc = 1.677 g/cm3, F(000) = 2280 and μ = 1.120 mm-1. Complex 1 possesses a thick and undulated two-dimensional layer with (6,3) topology, which interpenetrates with two others (above and below) to extend into a three-di- mensional supramolecular framework.

  17. Construction program management

    CERN Document Server

    Delaney, Joseph

    2013-01-01

    Although construction is one of the largest industries in the United States, it lags behind other industries in its implementation of modern management techniques such as those contained in the Standard for Program Management (the Standard) by the Project Management Institute (PMI(R)). Construction Program Management details the successful use of the PMI(R) approach for the construction of capital programs. It demonstrates, through case studies, how implementation of PMI's set of tools and techniques can improve the chances of program success. Exploring tactical and strategic management method

  18. Governmentalities of Construction

    DEFF Research Database (Denmark)

    Gottlieb, Stefan; Jensen, Jens Stissing

    2016-01-01

    In this paper, we apply Foucault’s concept of governmentality in a dual analysis of the formation and transformation of the construction sector and the construction worker. The governmentality concept is well-suited for such an analysis as it directs attention to the ways in which control...... is the exercised over a specific area of institutional life through the shaping of individuals’ conduct. We argue that construction, as a coherent sector, first was rendered governable in the 1940s in order to achieve national modernisation. It is shown how the political measures that were based on the exercise...

  19. Sustainability Base Construction Update

    Science.gov (United States)

    Mewhinney, Michael

    2012-01-01

    Construction of the new Sustainability Base Collaborative support facility, expected to become the highest performing building in the federal government continues at NASA's Ames Research Center, Moffet Field, Calif. The new building is designed to achieve a platinum rating under the leadership in Energy and Environment Design (LEED) new construction standards for environmentally sustainable construction developed by the U. S. Green Building Council, Washington, D. C. When completed by the end of 2011, the $20.6 million building will feature near zero net energy consumption, use 90 percent less potable water than conventionally build buildings of equivalent size, and will result in reduced building maintenance costs.

  20. Construction and Demolition Waste

    DEFF Research Database (Denmark)

    Christensen, Thomas Højlund; Andersen, L.

    2011-01-01

    Construction and demolition waste (C&D waste) is the waste generated during the building, repair, remodeling or removal of constructions. The constructions can be roads, residential housing and nonresidential buildings. C&D waste has traditionally been considered without any environmental problems...... and has just been landfilled. However, in recent years more focus has been put on C&D waste and data are starting to appear. One reason is that it has been recognized that C&D waste may include many materials that are contaminated either as part of their original design or through their use and therefore...

  1. Constructing supramolecular nanostructure by hydrogen-bonding

    Institute of Scientific and Technical Information of China (English)

    LI YiBao; ZENG QingDao; WANG ZhiHui; QI GuiCun; GUAN Li; FAN XiaoLin; WANG Chen

    2008-01-01

    The diquinoxalino (2.3-2'.3'-a.c) phenazine (DQP), containing 6 nitrogen atoms, was synthesized, and its adsorption and self-assembling behavior on highly oriented pyrolytic graphite (HOPG) was studied by scanning tunneling microscopy (STM) under ambient conditions. With 1,14-tetradecanedioic acid as a bridge, uniform two-dimensional arrays of 1,14-tetradecanedioic acid/DQP nanostrueture were suc-cessfully fabricated. The result illustrates that it is possible to construct and control supramolecular nanostructure by intermolecular hydrogen-bonding.

  2. Management for Construction Materials and Control of Construction Waste in Construction Industry: A Review

    Directory of Open Access Journals (Sweden)

    A. A. Gulghane

    2015-04-01

    Full Text Available In recent treads a wide range of building materials is available for the construction of civil engineering structures. The total cost of materials may be up to 60% or more of the total cost incurred in construction project dependent upon the type of project. Effective construction materials management is a key to success for a construction project. Construction waste is another serious problem in construction industry. A large and various types of construction waste with different characteristics are created at all the stages of construction. Construction industries have a larger part in contributing environmental problems. The economic and environmental benefits must be gained from construction waste minimization. This paper presents a review on systematically investigation of the management of construction materials and construction waste, material management techniques, control of construction waste and existing situation of construction management and construction waste in the industry.

  3. 高产琥珀酸重组大肠杆菌的构建及厌氧发酵%Construction and anaerobic fermentation of genetically engineered Escherichia coli for the production of succinic acid

    Institute of Scientific and Technical Information of China (English)

    赵锦芳; 华渤文; 王永泽; 赵筱; 王金华

    2013-01-01

    In this study, metabolic pathway engineering using Red recombinase system was performed on Escherichia coli W, a wild type strain, for the production of succinic acid. In order to eliminate the by-product formation of E. coli fermentation, five genes leading for production of lactate (ldhA) , formate. (pflB) ethanol (adhE) , and acetate (poxB and ackA) were deleted from the chromosome. The resulting mutant was then further selected through a-daptive (metabolic) evolution for improved anaerobic growth. The evolved strain WS100 could produce succinate as the major fermentation product. In a 15 L fermentation under anaerobic condition using mineral salt medium, WS100 produced 70. 13 g/L succinate from 100 g/L glucose in 72 h, with a volumetric productivity of 0. 98 g/( L · h) and a yield of 76% based on sugar metabolism. Although small amount of acetate and lactate were still produced as the minor by - products, there was no detectable production of formate and ethanol. This result demonstrated the great potential of the engineered E. coli WS100 for fermentative production of succinic acid at large scale.%以野生型大肠杆菌Escherichia coli W为出发菌株,利用Red同源重组系统分别敲除了乳酸脱氢酶基因(ldhA)、乙醇脱氢酶基因(adhE)、丙酮酸甲酸裂解酶基因(pflB)、丙酮酸氧化酶基因(poxB)和乙酸激酶基因(ackA),再通过无氧生长进化筛选过程,构建得到在厌氧条件下能有效生长,并以琥珀酸为主要发酵产物的重组大肠杆菌WS100(△ldhA,△adhE,△pflB,△poxB,△ackA).利用15 L发酵罐进行厌氧发酵测定显示,经72 h发酵,菌体密度OD600最大值可提高至6.48,琥珀酸产量达到70.13 g/L,琥珀酸的生产强度为0.98 g/(L· h),葡萄糖-琥珀酸转化率为76%.发酵液中副产物含量低,乙酸含量为5.34 g/L,乳酸产量仅为0.15 g/L,未检测到甲酸和乙醇生成.结果表明,厌氧条件下,该工程菌可有效利用低营养成分的无机盐培养基,在

  4. Utilization of mixed ligands to construct diverse Ni(II)-coordination polymers based on terphenyl-2,2‧,4,4‧-tetracarboxylic acid and varied N-donor co-ligands

    Science.gov (United States)

    Wang, Chao; Zhao, Jun; Xia, Liang; Wu, Xue-Qian; Wang, Jian-Fang; Dong, Wen-Wen; Wu, Ya-Pan

    2016-06-01

    Three new coordination polymers, namely, {[Ni(H2L)(bix)(H2O)2]·2h2O}n (1), {[Ni(HL)(Hdpa)(H2O)2]·H2O}n (2), {[Ni(L)0.5(bpp)(H2O)]·H2O}n (3) (H4L=terphenyl-2,2‧,4,4‧-tetracarboxylic acid; bix=1,4-bis(imidazol-1-ylmethyl)benzene; dpa =4,4‧-dipyridylamine; bpp=1,3-bis(4-pyridyl)propane), based on rigid H4L ligand and different N-donor co-ligands, have been synthesized under hydrothermal conditions. Compound 1 features a 3D 4-connected 66-dia-type framework with H4L ligand adopts a μ2-bridging mode with two symmetry-related carboxylate groups in μ1-η1:η0 monodentate mode. Compound 2 displays a 1D [Ni(HL)(Hdpa)]n ribbon chains motif, in which the H4L ligand adopts a μ2-bridging mode with two carboxylate groups in μ1-η1:η1 and μ1-η1:η0 monodentate modes, while 3 possesses a (4,4)-connected 3D frameworks with bbf topology, with H4L ligand displays a μ4-bridging coordination mode. The H4L ligand displays not only different deprotonated forms but also diverse coordination modes and conformations. The structural diversities among 1-3 have been carefully discussed, and the roles of N-donor co-ligands in the self-assembly of coordination polymers have been well documented.

  5. A novel copper(II) complex constructed with mixed ligands of biphenyl-4,4'-dicarboxylic acid (H 2bpdc) and dipyrido[3,2-d:2',3'-f]quinoxaline (Dpq): Synthesis, structure, electrochemistry and electrocatalysis

    Science.gov (United States)

    Lin, HongYan; Wang, XiuLi; Hu, HaiLiang; Chen, BaoKuan; Liu, GuoCheng

    2009-03-01

    A novel metal-organic framework [Cu 2(bpdc) 2(Dpq) 2(H 2O)]·H 2O ( 1) has been obtained from hydrothermal reaction of copper chloride with the mixed ligands [biphenyl-4,4'-dicarboxylic acid (H 2bpdc) and dipyrido[3,2-d:2',3'-f]quinoxaline (Dpq)], and structurally characterized by elemental analysis, IR, TG and single-crystal X-ray diffraction analysis. The unique feature is that there simultaneously exist two kinds of one-dimensional (1-D) zigzag polymeric chains in complex 1. Moreover, the 1-D polymeric chains are ultimately packed into a three-dimensional (3-D) supramolecular framework through two different hydrogen bonding interactions. The adjacent different chains are linked by C-H⋯O hydrogen bonding interactions, and the same kind chains are further connected through C-H⋯π stacking interactions. Additionally, the complex 1 was used as solid bulk-modifier to fabricate renewable carbon paste electrode (Cu-CPE) by the direct mixing method. The electrochemical behavior and electrocatalysis of Cu-CPE have been studied in detail. The results indicate that Cu-CPE give one-electron quasi-reversible redox waves in potential range of 400 to -300 mV due to the metal copper ion Cu(II)/Cu(I). The Cu-CPE showed good electrocatalytic activity toward the reduction of the bromate, nitrite and hydrogen peroxide. The electrocatalytic reduction peak currents of KBrO 3, KNO 2 and H 2O 2 showed a linear dependence on their concentrations. All of the results revealed that the Cu-CPE had a good reproducibility, remarkable long-term stability and especially good surface renewability by simple mechanical polishing in the event of surface fouling, which is important for practical application.

  6. A New 3D Layered-pillared Cobalt(II)-organic Framework Constructed by Imidazole-4,5-dicarboxylic Acid (H_3IDC) and 4,4'-Bipyridine (bipy)

    Institute of Scientific and Technical Information of China (English)

    YUAN Li; LU Wen-Guan

    2011-01-01

    A new 3D metal-organic framework of {[Co3(IDC)2(bipy)2(py)2]·7H2O}n (1) was obtained by the hydrothermal reaction of Co(NO3)2·6H2O, imidazole-4,5-dicarboxylic acid (H3IDC), 4,4'-bipyridine (bipy), and pyridine (py), and structurally characterized by elemental analysis, infrared spectroscopy, thermal gravimetric analysis, and single-crystal X-ray diffraction. X-ray diffraction crystal structural analysis reveals that it crystallizes in orthorhombic system, space group Pccn with a = 11.1040(3), b = 19.8834(5), c = 21.3025(5), V = 4703.3(2)3, Mr = 1079.63, Z = 4, Dc = 1.525 Mg·m-3, F(000) = 2212, μ(CuKα) = 8.855 mm-1, the final R = 0.0331 and wR = 0.0713 for 2525 observed reflections with I ≥ 2σ(I). In compound 1, each cobalt(II) ion is six-coordinated with a slightly distorted octahedral coordination geometry, and each μ3-IDC3- acts as a bridge to bond three neighboring Co(II) ions, leading to an infinite 2D network layer structure of [Co3(IDC)2]n with Kagomé lattice. The adjacent layers are further linked by μ2-bipy to form an infinite 3D layered-pillared framework architecture of [Co3(IDC)2(bipy)2(py)2]n.

  7. Passive solar construction handbook

    Energy Technology Data Exchange (ETDEWEB)

    Levy, E.; Evans, D.; Gardstein, C.

    1981-08-01

    Many of the basic elements of passive solar design are reviewed. The unique design constraints presented in passive homes are introduced and many of the salient issues influencing design decisions are described briefly. Passive solar construction is described for each passive system type: direct gain, thermal storage wall, attached sunspace, thermal storage roof, and convective loop. For each system type, important design and construction issues are discussed and case studies illustrating designed and built examples of the system type are presented. Construction details are given and construction and thermal performance information is given for the materials used in collector components, storage components, and control components. Included are glazing materials, framing systems, caulking and sealants, concrete masonry, concrete, brick, shading, reflectors, and insulators. The Load Collector Ratio method for estimating passive system performance is appended, and other analysis methods are briefly summarized. (LEW)

  8. Employment and winter construction

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan de Place; Larsen, Jacob Norvig

    2011-01-01

    possibilities exist for reducing seasonal variation in employment? In addition to a literature review related to winter construction, European and national employment and meteorological data were studied. Finally, ministerial acts, ministerial orders or other public policy documents related to winter...

  9. School Construction Scheduling.

    Science.gov (United States)

    Delaney, J. B.

    1983-01-01

    Explains that favorable market and working conditions influence the scheduling of school construction projects. Facility planners, architects, and contractors are advised to develop a realistic time schedule for the entire project. (MLF)

  10. Vascularised Tissue Engineering Construct

    Directory of Open Access Journals (Sweden)

    Irza Sukmana

    2012-01-01

    Full Text Available The guidance of endothelial cell organization into a capillary network has been a long-standing challenge in tissue engineering. Some research efforts have been made to develop methods to promote capillary networks inside engineered tissue constructs. Capillary and vascular networks that would mimic blood microvessel function can be used to subsequently facilitate oxygen and nutrient transfer as well as waste removal. Vascularization of engineering tissue construct is one of the most favorable strategies to overpass nutrient and oxygen supply limitation, which is often the major hurdle in developing thick and complex tissue and artificial organ. This paper addresses recent advances and future challenges in developing three-dimensional culture systems to promote tissue construct vascularization allowing mimicking blood microvessel development and function encountered in vivo. Bioreactors systems that have been used to create fully vascularized functional tissue constructs will also be outlined.

  11. Predictable grammatical constructions

    DEFF Research Database (Denmark)

    Lucas, Sandra

    2015-01-01

    My aim in this paper is to provide evidence from diachronic linguistics for the view that some predictable units are entrenched in grammar and consequently in human cognition, in a way that makes them functionally and structurally equal to nonpredictable grammatical units, suggesting...... that these predictable units should be considered grammatical constructions on a par with the nonpredictable constructions. Frequency has usually been seen as the only possible argument speaking in favor of viewing some formally and semantically fully predictable units as grammatical constructions. However, this paper...... semantically and formally predictable. Despite this difference, [méllo INF], like the other future periphrases, seems to be highly entrenched in the cognition (and grammar) of Early Medieval Greek language users, and consequently a grammatical construction. The syntactic evidence speaking in favor of [méllo...

  12. Ship construction and welding

    CERN Document Server

    Mandal, Nisith R

    2017-01-01

    This book addresses various aspects of ship construction, from ship types and construction materials, to welding technologies and accuracy control. The contents of the book are logically organized and divided into twenty-one chapters. The book covers structural arrangement with longitudinal and transverse framing systems based on the service load, and explains basic structural elements like hatch side girders, hatch end beams, stringers, etc. along with structural subassemblies like floors, bulkheads, inner bottom, decks and shells. It presents in detail double bottom construction, wing tanks & duct keels, fore & aft end structures, etc., together with necessary illustrations. The midship sections of various ship types are introduced, together with structural continuity and alignment in ship structures. With regard to construction materials, the book discusses steel, aluminum alloys and fiber reinforced composites. Various methods of steel material preparation are discussed, and plate cutting and form...

  13. On Construction Grammar

    Institute of Scientific and Technical Information of China (English)

    XIAO Kunxue

    2005-01-01

    Constructionist approach with its brand-new perspective has begun to demonstrate its dynamic power. This paper attempts to review the basic ideas, achievements and comparison with generative grammar of Construction Grammar and generalize some problems and future research prospects.

  14. Constructing the Audience

    DEFF Research Database (Denmark)

    Willig, Ida

    2010-01-01

    constructions in policy papers of Danish newspapers: the reader-as-citizen, which can be traced back to the late 1940s, and the reader-as-consumer, visible in the historically new reader profiles, where we also find a third reader construction, the reader-as-commodity. The development indicates a transformation...... from an Omnibus press system with a publicist logic of practice to a Segment press system with a commercial logic of practice....

  15. Constructing quantum codes

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Quantum error correcting codes are indispensable for quantum information processing and quantum computation.In 1995 and 1996,Shor and Steane gave first several examples of quantum codes from classical error correcting codes.The construction of efficient quantum codes is now an active multi-discipline research field.In this paper we review the known several constructions of quantum codes and present some examples.

  16. CONTRACT CONSTRUCTION ACTIVITY

    Directory of Open Access Journals (Sweden)

    ANTOANETA CLAUDIA BUTUZA

    2010-01-01

    Full Text Available This paper presents the main types of contractual arrangements used worldwide in construction: traditional contracts, cost reimbursable type contracts, management contracts and other contractual arrangements such as partnership, offering two-phase, contracts in series and turnkey contracts. Also, based on a comparison of the main types of contractual arrangements a number of recommendations are suggested for choosing the appropriate type of contract construction project to be realized.

  17. TANK CAR CONSTRUCTION REFINMENT

    Directory of Open Access Journals (Sweden)

    A. N. Soberzhansjkyj

    2010-06-01

    Full Text Available The increase of volume and load-carrying capacity of tank cars is an urgent task for improving the efficiency of transportation of liquid bulk cargoes. Variants of the constructive and technical approaches, which allow increasing the specified indices, are considered. After the analysis the most rational constructive scheme meeting the modern requirements for tank cars and allowing to raise their productivity is chosen.

  18. ATLAS construction status

    CERN Document Server

    Jenni, P

    2006-01-01

    The ATLAS detector is being constructed at the LHC, in view of a data-taking start-up in 2007. This report concentrates on the progress and the technical challenges of the detector construction, and summarizes the status of the work as of August 2004. The project is on track to allow the highly motivated ATLAS collaboration to enter into a new exploratory domain of high-energy physics in 2007.

  19. Valproic Acid

    Science.gov (United States)

    ... acid is in a class of medications called anticonvulsants. It works by increasing the amount of a ... older (about 1 in 500 people) who took anticonvulsants such as valproic acid to treat various conditions ...

  20. Ascorbic Acid

    Science.gov (United States)

    Ascorbic acid is used to prevent and treat scurvy, a disease caused by a lack of vitamin C ... Ascorbic acid comes in extended-release (long-acting) capsules and tablets, lozenges, syrup, chewable tablets, and liquid drops ...

  1. Amino acids

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/002222.htm Amino acids To use the sharing features on this page, please enable JavaScript. Amino acids are organic compounds that combine to form proteins . ...

  2. Construction of a yeast artificial chromosome contig encompassing the human acidic fibroblast growth factor (FGF1) gene: Toward the cloning of the ANLL/MDS tumor-suppressor gene

    Energy Technology Data Exchange (ETDEWEB)

    Chiu, Ing-Ming; Gilmore, E.C.; Liu, Yang; Payson, R.A. (Ohio State Univ., Columbus, OH (United States))

    1994-02-01

    The region surrounding the human acidic fibroblast growth factor (FGF1) locus on chromosome 5q31 is of particular interest since it represents a critical region consistently lost in acute nonlymphocytic leukemia (ANLL) or myelodysplastic syndrome (MDS) patients who have a demonstrable deletion of the distal portion of the long arm of chromosome 5. It is proposed that an ANLL/MDS leukemia suppressor gene resides on 5q31. The authors have previously shown that the gene is most likely localized between FGF1 and PDGFRB/CSF1R loci. The region has also been linked to at least four other genetic diseases, Treacher Collins syndrome, diastrophic dysplasia, limb-girdle muscular dystrophy, and an autosomal dominant deafness, by linkage analysis. Here, they describe yeast artificial chromosomes (YAC) spanning 450 kb around the FGF1 gene. Six YAC clones were isolated from a human YAC library and their restriction enzyme maps were determined. The overlap of the clones with each other and with FGF1 cosmid and phage clones was characterized. Three of the YAC clones were found to contain the entire FGF1 gene, which spans more than 100 kb. Proximal and distal ends of several of these YAC clones were isolated for further overlap cloning. The proximal ends of both Y2 and Y4 were localized to previously isolated FGF1 DNA by sequence analysis. The distal ends of these two clones also hybridized to a human-hamster hybrid containing chromosome 5 as the only human genetic material. These results suggest that these YAC clones represent colinear DNA around the FGF1 locus. None of the YAC clones were found to contain the CD 14 and GRL genes, the closest known proximal and distal markers (relative to the centromere) to the FGF1 gene, respectively. This contig is useful for the overlap cloning of the 5q31 region and for reverse genetic strategies for the isolation of disease genes in the region. 46 refs., 7 figs., 5 tabs.

  3. Management for Construction Materials and Control of Construction Waste in Construction Industry: A Review

    OpenAIRE

    A. A. Gulghane; Prof P. V. Khandve

    2015-01-01

    In recent treads a wide range of building materials is available for the construction of civil engineering structures. The total cost of materials may be up to 60% or more of the total cost incurred in construction project dependent upon the type of project. Effective construction materials management is a key to success for a construction project. Construction waste is another serious problem in construction industry. A large and various types of construction waste with different...

  4. Construction and immunological identification of lactic acid bacteria vaccine to Helicobacter pylori HspA%幽门螺杆菌HspA乳酸菌疫苗构建及免疫反应性鉴定

    Institute of Scientific and Technical Information of China (English)

    张小娟; 王新彩; 段广才; 张荣光

    2013-01-01

    Objective To explore the expression and immune activity of Helicobacter pylori(Hp) heat shock protein A (HspA) in Lactococcus lactis(L.lactis)NZ3900,a food-grade delivery vehicle.Methods NZ3900/pNZ8110-hspA was constructed using gene recombination technique.Growth curves were plotted to study whether the growth of recombinant bacteria was affected after exogenous gene hspA cloned into L.lactis and whether the growth of empty bacteria,empty plasmid bacteria and recombinant bacteria was affected after treatment of nisin at different concentrations.Sodium dodecylsulfate polyacrglaide gel electrophoreses (SDS-PAGE) and tricine SDS-PAGE were used to detect HspA expressed in recombinant bacteria.Western blot was adopted to confirm whether the HspA expressed by transformant bacteria having immunoreactivity.Results The hspA gene was amplified from genome DNA of Hp MEL-Hp27 and then cloned in the E.coli-L,lactis shuttle vector pNZ8110 and transformed into E.coli MC1061.The positive recombinant plasmid was electro-transformed into L.lactis NZ3900.There was no obvious affection observed from the growth curve after exogenous gene hspA cloned into L.lactis NZ3900.The growth of recombinant bacteria was affected after Nisin treatment at different concentrations except for 10 ng/ml Nisin treatment,while the growth of empty bacteria,empty plasmid bacteria was not affected by different concentrations of nisin.No HspA strip was observed in SDS-PAGE and tricine SDS-PAGE analyses.Western blot analysis showed that HspA expressed by recombinant bacteria had good immunoreactivity.Conclusion The growth of recombinant bacteria was affected by the expressioin of HspA.%目的 探讨幽门螺杆菌(Hp)热休克蛋白A (HspA)在食品级乳酸乳球菌NZ3900菌株中的克隆表达及免疫反应性.方法 利用基因重组技术构建乳酸乳球菌重组子NZ3900/pNZ8110-hspA;绘制生长曲线,观察hspA插入对乳酸乳球菌重组子生长影响及乳酸链球菌素(Nisin)对重组子

  5. Construction time of PWRs

    Energy Technology Data Exchange (ETDEWEB)

    Carajilescov, Pedro; Moreira, Joao M.L., E-mail: pedro.carajilescov@ufabc.edu.b, E-mail: joao.moreira@ufabc.edu.b [Universidade Federal do ABC (UFABC), Santo Andre, SP (Brazil). Center of Engineering, Modeling and Applied Social Sciences

    2011-07-01

    The cost of electricity generated by nuclear power is greatly affected by the capital cost, which is dependent on the construction time of the plant. This work analyses the construction time of PWRs in several countries with different market structure and licensing experience. Countries which succeeded to establish a more collaborative environment among utilities, constructors, regulators, and energy planners through effective partnerships were able to build PWRs in shorter times. The construction time in Germany, France and Russia was around 80 months and in Japan, about 60 months. The envelope of 95% of the cases includes a range between 50 and 250 months of construction time. The evaluations show that construction time of PWRs has been longer for countries that did not hold the technology to build their own reactors, and depended on contracts with foreign suppliers. The nominal power of the reactors was considered a measure of plant size, technology complexity and standardization. Countries with standardized reactor designs (France, Japan and Russia) were able to build plants in shorter times. (author)

  6. Fatty acids - trans fatty acids

    Science.gov (United States)

    The data supporting a negative effect of dietary trans fatty acids on cardiovascular disease risk is consistent. The primary dietary sources of trans fatty acids include partially hydrogenated fat and rudiment fat. The adverse effect of trans fatty acids on plasma lipoprotein profiles is consisten...

  7. Construction of Selenomonas ruminantium with the Acetic Acid Generation Deficiency and Analysis on the Relative Enzyme Activity%反刍月形单胞菌乙酸生成缺陷株的构建及相关酶活性分析

    Institute of Scientific and Technical Information of China (English)

    苑学; 孙国权; 逢晓阳; 刘国文; 王哲

    2011-01-01

    [Objective] The research aimed to the construct the acetic acid generation deficiency strain of Selenomonas ruminantium and analyze the fermentation characteristic. [ Method] Based on the transposon tagging method, Selenomonas ruminantium ( recipient strain) was carried out the transposon mutagenesis by using the transposon donor strain E. Coli S17-1/pZJ25 :: Tn5. The zygote was screened by using the selective medium which included kanamycin and sodium fluoroacetate. [ Result] Seven transposition engineering strains which had the stable resistance to kanamycin and fluoroethanoic acid were screened. The mutant strain of Selenomonas ruminantium was carried out 16S rRNA identification and Tn5 PCR identification. Moreover, the specific activities of AK and PTA were analyzed. The mutant strain belonged to pta gene deletion and fluoroethanoic acid resistance one. [ Conclusion] The research laid the foundation for further studying the acetic acid cellular metabolic network and regulation of rumen microorganism in the ruminant animal.%[目的]构建反刍月形单胞菌乙酸生成的缺陷株并分析其发酵特性.[方法]应用转座子标签法,通过转座子供体菌E.coli S17-1/pZJ25::Tn5对受体菌反刍月形单胞菌进行转座子诱变,采用含卡邢霉素和氟乙酸纳的选择性培养基筛选接合子.[结果]共筛选出稳定的对卡那霉素和氟乙酸具有抗性的转座工程菌7株.对反刍月形单胞菌的突变株进行16S rRNA鉴定和Tn5的PCR鉴定,及乙酸激酶(AK)和磷酸乙酰转移酶(PTA)酶比活力分析,确定突变株属于pta基因缺失型氟乙酸抗性菌株.[结论]该研究为进一步研究反刍善瘤胃微生物乙酸的细胞代谢网络和调控奠定基础.

  8. Novel genetic tools for diaminopimelic acid selection in virulence studies of Yersinia pestis.

    Directory of Open Access Journals (Sweden)

    David M Bland

    Full Text Available Molecular studies of bacterial virulence are enhanced by expression of recombinant DNA during infection to allow complementation of mutants and expression of reporter proteins in vivo. For highly pathogenic bacteria, such as Yersinia pestis, these studies are currently limited because deliberate introduction of antibiotic resistance is restricted to those few which are not human treatment options. In this work, we report the development of alternatives to antibiotics as tools for host-pathogen research during Yersinia pestis infections focusing on the diaminopimelic acid (DAP pathway, a requirement for cell wall synthesis in eubacteria. We generated a mutation in the dapA-nlpB(dapX operon of Yersinia pestis KIM D27 and CO92 which eliminated the expression of both genes. The resulting strains were auxotrophic for diaminopimelic acid and this phenotype was complemented in trans by expressing dapA in single and multi-copy. In vivo, we found that plasmids derived from the p15a replicon were cured without selection, while selection for DAP enhanced stability without detectable loss of any of the three resident virulence plasmids. The dapAX mutation rendered Y. pestis avirulent in mouse models of bubonic and septicemic plague which could be complemented when dapAX was inserted in single or multi-copy, restoring development of disease that was indistinguishable from the wild type parent strain. We further identified a high level, constitutive promoter in Y. pestis that could be used to drive expression of fluorescent reporters in dapAX strains that had minimal impact to virulence in mouse models while enabling sensitive detection of bacteria during infection. Thus, diaminopimelic acid selection for single or multi-copy genetic systems in Yersinia pestis offers an improved alternative to antibiotics for in vivo studies that causes minimal disruption to virulence.

  9. Rural Infrastructure Construction in New Socialist Countryside Construction

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    In the background of new socialist countryside construction,rural infrastructure construction is favorable to consolidating fundamental position of agriculture,expanding rural job opportunities,increasing farmers’ income,and favorable to development of rural economy and the national economy. Rural infrastructure construction mainly includes construction of agricultural basic production conditions,construction of rural ecological environment,construction of agricultural service system,and construction of rural living facilities. Finally,we put forward approaches and methods for strengthening rural infrastructure construction: firstly,increase fund input to provide financial support for rural infrastructure construction; second ly,make scientific village planning to ensure sustainability of rural infrastructure construction; thirdly,arouse farmers’ enthusiasm and initiative to promote stable development of rural infrastructure construction.

  10. Reliability and construction control

    Directory of Open Access Journals (Sweden)

    Sherif S. AbdelSalam

    2016-06-01

    Full Text Available The goal of this study was to determine the most reliable and efficient combination of design and construction methods required for vibro piles. For a wide range of static and dynamic formulas, the reliability-based resistance factors were calculated using EGYPT database, which houses load test results for 318 piles. The analysis was extended to introduce a construction control factor that determines the variation between the pile nominal capacities calculated using static versus dynamic formulae. From the major outcomes, the lowest coefficient of variation is associated with Davisson’s criterion, and the resistance factors calculated for the AASHTO method are relatively high compared with other methods. Additionally, the CPT-Nottingham and Schmertmann method provided the most economic design. Recommendations related to a pile construction control factor were also presented, and it was found that utilizing the factor can significantly reduce variations between calculated and actual capacities.

  11. Applying lean thinking in construction

    OpenAIRE

    Remon Fayek Aziz; Sherif Mohamed Hafez

    2013-01-01

    The productivity of the construction industry worldwide has been declining over the past 40 years. One approach for improving the situation is using lean construction. Lean construction results from the application of a new form of production management to construction. Essential features of lean construction include a clear set of objectives for the delivery process, aimed at maximizing performance for the customer at the project level, concurrent design, construction, and the application of...

  12. La Grammaire de Construction

    OpenAIRE

    Yannick Mathieu, Yvette

    2003-01-01

    Cet article est une introduction à la Grammaire de Construction (notée GC dans la suite) développée par Charles. J. Fillmore et Paul Kay (Fillmore (1985), Fillmore, Kay & O’Connor (1988), Kay (1994), Kay & Fillmore (1999)). Cette approche s’inscrit dans un courant général qui attribue un rôle central à la notion de construction grammaticale (Lakoff (1987), Zwicky (1987), Langacker (1987), Fried (à paraître), Goldberg (1995), Lambrecht (1995, 2001), etc.). Les exemples présentés en anglais so...

  13. Logic, construction, computation

    CERN Document Server

    Berger, Ulrich; Diener, Hannes; Seisenberger, Monika

    2012-01-01

    Over the last few decades the interest of logicians and mathematicians in constructive and computational aspects of their subjects has been steadily growing, and researchers from disparate areas realized that they can benefit enormously from the mutual exchange of techniques concerned with those aspects. A key figure in this exciting development is the logician and mathematician Helmut Schwichtenberg to whom this volume is dedicated on the occasion of his 70th birthday and his turning emeritus. The volume contains 20 articles from leading experts about recent developments in Constructive set t

  14. Constructing populations in biobanking

    DEFF Research Database (Denmark)

    Tupasela, Aaro; Snell, Karoliina; Cañada, Jose a.

    2015-01-01

    This article poses the question of whether biobanking practices and standards are giving rise to the construction of populations from which various biobanking initiatives increasingly draw on for legitimacy? We argue that although recent biobanking policies encourage various forms of engagement...... to the construction of populations, whereby specific nationalities, communities, societies, patient groups and political systems become imbued or bio-objectified with particular characteristics, such as compliant, distant, positive, commercialized or authoritarian. This bio-objectification process is problematic...... in relation to policy aspirations ascribed to biobanking engagement since it gives rise to reified notions of different populations....

  15. Federal Environmental Requirements for Construction

    Data.gov (United States)

    Department of Veterans Affairs — This guide provides information on federal environmental requirements for construction projects. It is written primarily for owners of construction projects and for...

  16. Utilization of acidic α-amino acids as acyl donors: an effective stereo-controllable synthesis of aryl-keto α-amino acids and their derivatives.

    Science.gov (United States)

    Wang, Lei; Murai, Yuta; Yoshida, Takuma; Okamoto, Masashi; Tachrim, Zetryana Puteri; Hashidoko, Yasuyuki; Hashimoto, Makoto

    2014-05-16

    Aryl-keto-containing α-amino acids are of great importance in organic chemistry and biochemistry. They are valuable intermediates for the construction of hydroxyl α-amino acids, nonproteinogenic α-amino acids, as well as other biofunctional components. Friedel-Crafts acylation is an effective method to prepare aryl-keto derivatives. In this review, we summarize the preparation of aryl-keto containing α-amino acids by Friedel-Crafts acylation using acidic α-amino acids as acyl-donors and Lewis acids or Brönsted acids as catalysts.

  17. Ibotenic acid and thioibotenic acid

    DEFF Research Database (Denmark)

    Hermit, Mette B; Greenwood, Jeremy R; Nielsen, Birgitte

    2004-01-01

    In this study, we have determined and compared the pharmacological profiles of ibotenic acid and its isothiazole analogue thioibotenic acid at native rat ionotropic glutamate (iGlu) receptors and at recombinant rat metabotropic glutamate (mGlu) receptors expressed in mammalian cell lines....... Thioibotenic acid has a distinct pharmacological profile at group III mGlu receptors compared with the closely structurally related ibotenic acid; the former is a potent (low microm) agonist, whereas the latter is inactive. By comparing the conformational energy profiles of ibotenic and thioibotenic acid...... with the conformations preferred by the ligands upon docking to mGlu1 and models of the other mGlu subtypes, we propose that unlike other subtypes, group III mGlu receptor binding sites require a ligand conformation at an energy level which is prohibitively expensive for ibotenic acid, but not for thioibotenic acid...

  18. Energy-Friendly Construction

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A building is a base for human activities, but it also consumes a large amount of energy and resources. Of all the newly constructed buildings throughout China, it is estimated only 10 to 15 percent are up to the state standard, and over 80 percent require enormous energy consumption. This

  19. ACCELERATING CONSTRUCTION INDUSTRY DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    Mohan M. Kumaraswamy

    2006-06-01

    Full Text Available The needs for construction industry development are initially viewed from the broader perspectives of imperatives for infrastructure development and national development. All these are clearly more critical in developing countries. A non-exhaustive set of potential drivers and common barriers to construction industry development is identified from previous research. These suggest the usefulness of consolidating a cluster of recent proposals and exercises aiming at (a construction organization development in terms of an over-arching management support system model, as well as improved information and knowledge management; and (b project team development in the context of relationally integrated teams and supply chains, joint risk management and ‘technology and knowledge exchange’ in joint ventures, as well as longer term public private partnerships. These apparently disparate research thrusts are threaded together into a pattern that may inspire, if not feed, specific research and development (R & D agendas for construction industry development in different countries according to their own priorities, constraints and stages of infrastructure and national development.

  20. Intentionality Versus Constructive Empiricism

    NARCIS (Netherlands)

    F.A.I. Buekens; F.A. Muller (Archibald)

    2012-01-01

    textabstractBy focussing on the intentional character of observation in science, we argue that Constructive Empiricism-B. C. van Fraassen's much debated and explored view of science-is inconsistent. We then argue there are at least two ways out of our Inconsistency Argument, one of which is more eas

  1. Construction of Resilient Functions

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jie; WEN Qiao-yan

    2005-01-01

    Based on the relationship between nonlinearity and resiliency of a multi-output function, we present a method for constructing nonintersecting linear codes from packing design. Through these linear codes, we obtain n-variable, moutput, t-resilient functions with very high nonlinearity.Their nonlinearities are currently the best results for most of cases.

  2. Constructions of Peripherality

    DEFF Research Database (Denmark)

    Carter, Helen Frances Lindsay

    2013-01-01

    In this paper I focus on the concept of peripheralisation. In particular, I consider how peripheries can be discursively constructed in the debates surrounding planning cases, and how this might serve to legitimate particular interests. This is related to the case of a proposed golf resort...

  3. Mortality table construction

    Science.gov (United States)

    Sutawanir

    2015-12-01

    Mortality tables play important role in actuarial studies such as life annuities, premium determination, premium reserve, valuation pension plan, pension funding. Some known mortality tables are CSO mortality table, Indonesian Mortality Table, Bowers mortality table, Japan Mortality table. For actuary applications some tables are constructed with different environment such as single decrement, double decrement, and multiple decrement. There exist two approaches in mortality table construction : mathematics approach and statistical approach. Distribution model and estimation theory are the statistical concepts that are used in mortality table construction. This article aims to discuss the statistical approach in mortality table construction. The distributional assumptions are uniform death distribution (UDD) and constant force (exponential). Moment estimation and maximum likelihood are used to estimate the mortality parameter. Moment estimation methods are easier to manipulate compared to maximum likelihood estimation (mle). However, the complete mortality data are not used in moment estimation method. Maximum likelihood exploited all available information in mortality estimation. Some mle equations are complicated and solved using numerical methods. The article focus on single decrement estimation using moment and maximum likelihood estimation. Some extension to double decrement will introduced. Simple dataset will be used to illustrated the mortality estimation, and mortality table.

  4. School Construction Summer Slam

    Science.gov (United States)

    Jensen, Richard F.

    2012-01-01

    Every school has a list of renovations, upgrades and repairs that need attention, but many are too distracting and disruptive to carry out during the school year. Often, the best time to address these nagging construction projects is during the summer when students are on break and the campus is quieter. Although these "summer slammers" often are…

  5. Provability Calculus of Constructions

    DEFF Research Database (Denmark)

    Nyblad, Kasten

    This thesis presents a type system, Provability Calculus of Constructions (PCoC) that can be used for the formalization of logic. In a theorem prover based on the system, the user can extend the prover with new inference rules in a logically consistent manner. This is done by representing PCo...

  6. Modular cleanroom construction success.

    Science.gov (United States)

    Möllmann, Markus

    2007-09-01

    The completion of a 408 m2 major new aseptic pharmacy unit for the St George's Hospital NHS Trust, London, is a significant example of the benefits of using modern modular construction techniques compared to a traditional cleanroom build. At every stage from concept through project planning to final completion, the use of modules proved to be the most appropriate for the task.

  7. Construction, repair and maintenance

    Energy Technology Data Exchange (ETDEWEB)

    Hinger, R. [Terasen Pipelines, Calgary, AB (Canada); Wong, P. [KC Integrity Corp., Calgary, AB (Canada); Yeomans, M.; Glover, A. [TransCanada PipeLines Ltd., Calgary, AB (Canada)

    2003-07-01

    The presentations by Working Group 4 focused on construction, repair and maintenance of pipelines with particular reference to high-strength steels and their applications for natural gas transmission pipelines. Discussions focused on how technology can be used to provide cost-effective design and construction solutions for long distance pipelines in a manner that does not compromise safety and reliability. The role that carbon plays in increasing the toughness of steel was also discussed. A few fundamental changes in steel production technology and a reduction in carbon content can result in a simultaneous increase in strength and toughness of steels. The cost impact of this technology was summarized. It was concluded that high strength steels offer effective solutions to deliver cost-effective pipelines to transport natural gas from northern basins. This session also presented pipeline routes that transport diluted bitumens from the Athabasca Oil Sands Project and the Muskeg River Mine to upgrader facilities in Alberta. Construction issues included design conservatism, water course crossings, winter construction, winter ditching, and public consultation. Examples of high strength steel pipe applications were also included with reference to experience gained by TransCanada and Corridor Pipeline. tabs., figs.

  8. The Seneca Amplification Construction

    Directory of Open Access Journals (Sweden)

    Wallace Chafe

    2012-01-01

    Full Text Available The polysynthetic morphology of the Northern Iroquoian languages presents a challenge to studies of clause combining. The discussion here focuses on a Seneca construction that may appear within a single clause but may also straddle clause boundaries. It amplifies the information provided by a referent, here called the trigger, that is introduced by the pronominal prefix within a verb or occasionally in some other way. The particle neh signals that further information about that referent will follow. This construction is found at four levels of syntactic complexity. At the first level the trigger and its amplification occur within the same prosodic phrase and the amplification is a noun. At the second level the amplification occurs in a separate prosodic phrase but remains a noun. At the third level the amplification exhibits verb morphology but has been lexicalized with a nominal function. At the fourth level the amplification functions as a full clause and neh serves as a marker of clause combining. Several varieties of amplification are discussed, as are cases in which the speaker judges that no amplification is needed. It is suggested that the typologically similar Caddo language illustrates a situation in which this construction could never arise, simply because Caddo verbs lack the pronominal element that triggers the construction in Seneca.

  9. Noncommutative Sugawara construction

    Energy Technology Data Exchange (ETDEWEB)

    Ghasemkhani, M. [Shahid Beheshti University, Department of Physics, Tehran (Iran, Islamic Republic of)

    2015-07-15

    The noncommutative extension of the Sugawara construction for free massless fermionic fields in two dimensions is studied. We prove that the equivalence of the noncommutative Sugawara energy-momentum tensor and symmetric energy-momentum tensor persists in the noncommutative extension. Some relevant physical results of this equivalence are also discussed. (orig.)

  10. Combustor liner construction

    Science.gov (United States)

    Craig, H. M.; Wagner, W. B.; Strock, W. J. (Inventor)

    1983-01-01

    A combustor liner is fabricated from a plurality of individual segments each containing counter/parallel Finwall material and are arranged circumferentially and axially to define the combustion zone. Each segment is supported by a hook and ring construction to an opened lattice frame with sufficient tolerance between the hook and ring to permit thermal expansion with a minimum of induced stresses.

  11. Construction Report, 2000.

    Science.gov (United States)

    Abramson, Paul

    2000-01-01

    Provides cost data on college and university construction projects completed for 1999 and projected for completion in 2000 by region. New building cost comparisons and profiles are listed, including basic information on 61 dormitory projects schedules to be completed in 2000. (GR)

  12. KARATE WITH CONSTRUCTIVE LEARNING

    Directory of Open Access Journals (Sweden)

    Srikrishna Karanam

    2012-02-01

    Full Text Available Any conventional learning process involves the traditional hierarchy of garnering of information and then recall gathered information. Constructive learning is an important research area having wide impact on teaching methods in education, learning theories, and plays a major role in many education reform movements. It is observed that constructive learning advocates the interconnection between emotions and learning. Human teachers identify the emotions of students with varying degrees of accuracy and can improve the learning rate of the students by motivating them. In learning with computers, computers also should be given the capability to recognize emotions so as to optimize the learning process. Image Processing is a very popular tool used in the process of establishing the theory of Constructive Learning. In this paper we use the Optical Flow computation in image sequences to analyze the accuracy of the moves of a karate player. We have used the Lucas-Kanade method for computing the optical flow in image sequences. A database consisting of optical flow images by a group of persons learning karate is formed and the learning rates are analyzed in order to main constructive learning. The contours of flow images are compared with the standard images and the error graphs are plotted. Analysis of the emotion of the amateur karate player is made by observing the error plots.

  13. Nanotechnologies for sustainable construction

    DEFF Research Database (Denmark)

    Geiker, Mette Rica; Andersen, Maj Munch

    2009-01-01

    This chapter aims to highlight key aspects and recent trends in the development and application of nanotechnology to facilitate sustainable construction, use and demolition of buildings and infrastructure structures, ‘nanoconstruction’. Nanotechnology is not a technology but a very diverse...

  14. Glider construction and design

    Science.gov (United States)

    Gymnich, Alfried

    1927-01-01

    The endeavor to draw a strict line between gliders and soarers as been recently abandoned and the following classification adopted: 1) gliders controlled by shifting the weight of the body; 2) gliders controlled by rudders; 3) gliders controlled by wings. The different classes are discussed as well as materials and parts used in construction.

  15. Industry Studies 2002: Construction

    Science.gov (United States)

    2002-01-01

    Jacksonville, FL Rotary Club of San Juan, San Juan, PR Tren Urbano Subway Project, San Juan, PR U.S. Army South, San Juan, PR U.S. Coast Guard Housing Project...are its implications for construction?, Foresight/CRISP Workshop on Nanotechnology, Royal Society of Arts . Cheltenham, England: 2001, p.5. 56 Concrete

  16. Okadaic acid

    DEFF Research Database (Denmark)

    Danielsen, E Michael; Hansen, Gert H; Severinsen, Mai C K

    2014-01-01

    Okadaic acid (OA) is a polyether fatty acid produced by marine dinoflagellates and the causative agent of diarrhetic shellfish poisoning. The effect of OA on apical endocytosis in the small intestine was studied in organ cultured porcine mucosal explants. Within 0.5-1 h of culture, the toxin caused...... in acidic organelles, implying a different toxic mechanism of action. We propose that rapid induction of LBs, an indicator of phospholipidosis, should be included in the future toxicity profile of OA....

  17. The Evolution of Modular Construction.

    Science.gov (United States)

    American School & University, 1993

    1993-01-01

    Explores how the myths of modular construction for schools began; also discusses the advances made in steel and modular construction. The major advantages of using permanent modular construction for schools are highlighted, including its rapid construction, use of standard building materials, financial flexibility, and durability. (GR)

  18. The Conjugate Acid-Base Chart.

    Science.gov (United States)

    Treptow, Richard S.

    1986-01-01

    Discusses the difficulties that beginning chemistry students have in understanding acid-base chemistry. Describes the use of conjugate acid-base charts in helping students visualize the conjugate relationship. Addresses chart construction, metal ions, buffers and pH titrations, and the organic functional groups and nonaqueous solvents. (TW)

  19. Lean Construction Implementation in the Saudi Arabian Construction Industry

    Directory of Open Access Journals (Sweden)

    Jamil Ghazi Sarhan

    2017-03-01

    Full Text Available The Kingdom of Saudi Arabia (KSA has witnessed a huge increase in construction during the last two decades. However, many projects experienced time delays, cost overruns and the generation of massive amounts of waste. To address these challenges, lean construction has been introduced into the Saudi construction industry; however, it is still in its infancy. This study therefore investigates the current state of lean construction implementation in the construction industry in the KSA. The objectives are to identify: the types of construction waste, level of use of tools that support the implementation of lean construction, stages of application of lean methods, and the benefits of lean construction. To achieve these objectives, a structured questionnaire survey of 282 construction professionals was carried out. After the analysis of the collected data using mean score and Anova test, the following conclusions were made.  In the construction industry in the KSA, waiting is the most common type of waste, while Computer Aided Design (CAD is the conventional tool supporting the implementation of lean construction. Furthermore, the data suggests that lean construction is most commonly used in the construction stage of projects while customer satisfaction is the main benefit derived from lean construction practices. This study concludes that the level of implementation of lean construction in the KSA construction industry is increasing. The results will help benchmark the current state of lean construction implementation, which will enable the construction industry to identify strategies to implement lean construction in Saudi Arabia in accordance with their needs and project goals, to achieve better productivity.

  20. The Specifications for Monitoring of Acid Rain

    Institute of Scientific and Technical Information of China (English)

    Tang Jie

    2011-01-01

    Background Since China is a country seriously affected by acid rain pollution,it is a long-term fundamental work for acid rain pollution prevention and control in China by getting well informed of the characteristics of spatial and temporal changes in acid rain and long-term trends of these changes.In order to reach the national demand for acid rain monitoring data,the China Meteorological Administration (CMA) began to construct the network of acid rain monitoring stations in 1992.By the end of 2010,the total number of monitoring stations has exceeded 340.

  1. Folic Acid

    Science.gov (United States)

    ... damage. 10 Do I need folic acid after menopause? Yes. Women who have gone through menopause still need 400 micrograms of folic acid every ... United States: 2003–2006 . American Journal of Clinical Nutrition; 91(1): 231–237. Hamner, H.C., Cogswell, ...

  2. Construction with macromolecules

    Directory of Open Access Journals (Sweden)

    Stefan Hecht

    2005-03-01

    Full Text Available The generation of nanosized features via the bottom-up organization of individual atoms and molecules represents one of the most rapidly emerging fields in modern science and technology. In order to gain general and modular access to nanostructures, a construction kit consisting of molecular building blocks with defined nanoscale dimensions, shape, and functionality is required. While nature has developed a hierarchical strategy to construct sophisticated and complex nanostructures, chemistry is just starting to explore artificial macromolecules as a means to synthesize specific nano-objects. In this review, recent advances in achieving control over conformation within macromolecules and the interconnection of conformationally defined subunits by either covalent bonds or self-assembly are discussed in analogy to natural structure evolution.

  3. Constructive Engineering of Simulations

    Science.gov (United States)

    Snyder, Daniel R.; Barsness, Brendan

    2011-01-01

    Joint experimentation that investigates sensor optimization, re-tasking and management has far reaching implications for Department of Defense, Interagency and multinational partners. An adaption of traditional human in the loop (HITL) Modeling and Simulation (M&S) was one approach used to generate the findings necessary to derive and support these implications. Here an entity-based simulation was re-engineered to run on USJFCOM's High Performance Computer (HPC). The HPC was used to support the vast number of constructive runs necessary to produce statistically significant data in a timely manner. Then from the resulting sensitivity analysis, event designers blended the necessary visualization and decision making components into a synthetic environment for the HITL simulations trials. These trials focused on areas where human decision making had the greatest impact on the sensor investigations. Thus, this paper discusses how re-engineering existing M&S for constructive applications can positively influence the design of an associated HITL experiment.

  4. Construction quality assurance report

    Energy Technology Data Exchange (ETDEWEB)

    Roscha, V.

    1994-09-08

    This report provides a summary of the construction quality assurance (CQA) observation and test results, including: The results of the geosynthetic and soil materials conformance testing. The observation and testing results associates with the installation of the soil liners. The observation and testing results associated with the installation of the HDPE geomembrane liner systems. The observation and testing results associated with the installation of the leachate collection and removal systems. The observation and testing results associated with the installation of the working surfaces. The observation and testing results associated with in-plant manufacturing process. Summary of submittal reviews by Golder Construction Services, Inc. The submittal and certification of the piping material specifications. The observation and verification associated of the Acceptance Test Procedure results of the operational equipment functions. Summary of the ECNs which are incorporated into the project.

  5. Constructions in Wonderland

    DEFF Research Database (Denmark)

    Jensen, Kim Ebensgaard; Shibuya, Yoshikata

    In constructionist theory (e.g. Fillmore et al 1988; Goldberg 1995; Croft 2001), constructions are functional entities that pair form and conventionalized semantic and/or discourse-pragmatic function. In this paper, we will explore the extent to which the N-gram information retrieval technique......-gram analysis to some different texts, including Lewis Carroll's 1865 novel Alice's Adventures in Wonderland (AW), Mark Twain's 1884/5 novel The Adventures of Huckleberry Finn (HF), and some US presidential speeches, to identify frequently (co)occurring words that may be indicative of underlying constructions...... by the narrator and titular character, including the N-grams I reckon, there warn't no, I says to myself, and it warn't no use, thus contributing to his mind-style (Fowler 1977) and characterization. Moreover, a number of recurring strings are additionally imbued with narrative-structural functions, such as the 2...

  6. Applying lean thinking in construction

    Directory of Open Access Journals (Sweden)

    Remon Fayek Aziz

    2013-12-01

    Full Text Available The productivity of the construction industry worldwide has been declining over the past 40 years. One approach for improving the situation is using lean construction. Lean construction results from the application of a new form of production management to construction. Essential features of lean construction include a clear set of objectives for the delivery process, aimed at maximizing performance for the customer at the project level, concurrent design, construction, and the application of project control throughout the life cycle of the project from design to delivery. An increasing number of construction academics and professionals have been storming the ramparts of conventional construction management in an effort to deliver better value to owners while making real profits. As a result, lean-based tools have emerged and have been successfully applied to simple and complex construction projects. In general, lean construction projects are easier to manage, safer, completed sooner, and cost less and are of better quality. Significant research remains to complete the translation to construction of lean thinking in Egypt. This research will discuss principles, methods, and implementation phases of lean construction showing the waste in construction and how it could be minimized. The Last Planner System technique, which is an important application of the lean construction concepts and methodologies and is more prevalent, proved that it could enhance the construction management practices in various aspects. Also, it is intended to develop methodology for process evaluation and define areas for improvement based on lean approach principles.

  7. Construction Contract Provisions Analysis.

    Science.gov (United States)

    1982-09-01

    bidding and contract docuents. These documents appear together in the bid package. After the bid, the IFB (or RFTP, RFP ) is extracted and the remainder...Corps personnel suggest that the original reason for the clause -- to prevent brokering - is anachronistic in the current construction market. However...amount of premiums paid for performance and payment * bonds (including coinsurance and reinsurance agreements, when applicable) after furnishing

  8. Pseudorandomness and Combinatorial Constructions

    OpenAIRE

    2006-01-01

    In combinatorics, the probabilistic method is a very powerful tool to prove the existence of combinatorial objects with interesting and useful properties. Explicit constructions of objects with such properties are often very difficult, or unknown. In computer science, probabilistic algorithms are sometimes simpler and more efficient than the best known deterministic algorithms for the same problem. Despite this evidence for the power of random choices, the computational theory of pseudorandom...

  9. Construct of emotional intelligence

    Directory of Open Access Journals (Sweden)

    Sonja Pečjak in Andreja Avsec

    2003-04-01

    Full Text Available The article highlights the construct of emotional intelligence, that has appeared about then years ago. We present the popular and scientific comprehension of emotional intelligence, briefly describe the development of the concept and than in detail we propose the existing comprehension of emotional intelligence: through the models of Goleman (1995 and Bar-On (1997 we present the comprehension of emotional intelligence as a non-cognitive (personality traits.

  10. LEAR construction completed

    CERN Multimedia

    1982-01-01

    In July 1982, LEAR construction was completed, the individual systems had been dry-tested. On 16 July, the first 50 MeV (309 MeV/c) protons from Linac 1 were injected and circulated. On 11 October, the first antiprotons from the AA, decelerated in the PS to 609 MeV/c, were injected. Also in 1982, acceleration, deceleration and stochastic cooling were successfully tested. See 9007366 for a more detailed description. See also 8201061, 8204131, 8309026.

  11. Sustainable Construction in Afghanistan

    Science.gov (United States)

    2010-09-01

    described in the Szabo and Barfield book.74 These descriptions are summarized in the chart in Appendix C, Section 1. With the exception of Bamiyan , who...chubdara hut is also found in this area. It is also constructed of reeds, but has a wooden frame for roof support.79 Bamiyan is the home to the...Afghanistan currently uses caves for domestic housing, Bamiyan . It was common in the past in many regions; however, the practice has slowly disappeared as

  12. Construct Graph Logic

    OpenAIRE

    Tan, Yong

    2013-01-01

    In this paper, author uses set theory to construct a logic model of abstract figure from binary relation. Based on the uniform quantified structure, author gives two logic system for graph traversal and graph coloring respectively, moreover shows a new method of cutting graph. Around this model, there are six algorithms in this paper including exact graph traversal, Algebra calculation of natural number, graph partition and graph coloring.

  13. Self constructing in adolescence

    Directory of Open Access Journals (Sweden)

    Vranješević Jelena

    2003-01-01

    Full Text Available The aim of this study was to explore “theories” adolescents have about themselves, i.e. about the changes in the ways they construe themselves in the period of middle adolescence: how they construe changes and estimate them according to some dimensions relevant for change (appreciation of change, its importance, expectancy, timing, comprehensiveness, intensity and visibility in social surroundings. The study was explorative and it was performed on the sample of 96 adolescents aged 15-17 (middle adolescence. Instruments used for the study were: interview and Role Construct Repertory Grid, adjusted to the need of study. The results show that the adolescents’ construct system is highly monolithic and tight in regard to constructs (black and white construing, but at the same time vague and predictively unclear in regard to elements, i. e. the stated changes, meaning that adolescents are not able to construe those changes according to certain constructs. It seems that this study included the beginning of the loosening phase (creativity cycle in which the system is still tight and, therefore, protects from threat and anxiety, while the elements are not predictively (this is what Erikson calls moratorium, i.e. the process of active experimenting. Changes which are fully predictively are those which refer to adults' roles, such as: self confident, experienced, independent and has responsibilities, worries. On the hand, changes which are predictably the most clear to adolescents (and not appreciated are depression and nervousness. Adolescents face an implicative dilemma: positive implications of maturity are not enough, while negative implications, such as nervousness, depression and not trusting people are clear, predictable and negative. This dilemma can be the cause of their ambivalence towards growing-up and accepting the roles of adults.

  14. Canadian construction industry

    Energy Technology Data Exchange (ETDEWEB)

    Rich, M.

    2001-07-01

    The principal sectors of the Canadian construction industry - commercial, industrial, institutional and residential - are examined with regard to their technical considerations concerning the subject of sustainability. Apart from the different needs of each of the sectors of the industry there are also regional differences caused by population distribution, and differences in climate, that have to be identified and accommodated in considering attitudes to recycling and sustainable development. Some indications that there is growing awareness of recycling and reuse are: the increasing frequency of life cycle costing in the commercial and institutional sectors, the use of recycled or otherwise waste materials in concrete, examples of using steel supporting structures and roof joists salvaged from previous uncompleted projects in the industrial sector, improved building envelope and indoor air quality concerns, collective ground source heating, and new basement and framing technologies and construction materials in the residential sector. These improvements notwithstanding, there remains much to be done. The new objective-based National Building Code, for which comments are now being solicited across the country, is expected to identify new and innovative solutions and to kick-start serious efforts to come up with solutions towards increasing overall sustainability in all sectors of the Canadian construction industry.

  15. Toward a constructive physics

    Energy Technology Data Exchange (ETDEWEB)

    Noyes, H.P.; Gefwert, C.; Manthey, M.J.

    1983-06-01

    We argue that the discretization of physics which has occurred thanks to the advent of quantum mechanics has replaced the continuum standards of time, length and mass which brought physics to maturity by counting. The (arbitrary in the sense of conventional dimensional analysis) standards have been replaced by three dimensional constants: the limiting velocity c, the unit of action h, and either a reference mass (eg m/sub p/) or a coupling constant (eg G related to the mass scale by hc/(2..pi..Gm/sub p//sup 2/) approx. = 1.7 x 10/sup 38/). Once these physical and experimental reference standards are accepted, the conventional approach is to connect physics to mathematics by means of dimensionless ratios. But these standards now rest on counting rather than ratios, and allow us to think of a fourth dimensionless mathematical concept, which is counting integers. According to constructive mathematics, counting has to be understood before engaging in the practice of mathematics in order to avoid redundancy. In its strict form constructive mathematics allows no completed infinities, and must provide finite algorithms for the computation of any acceptable concept. This finite requirement in constructive mathematics is in keeping with the practice of physics when that practice is restricted to hypotheses which are testable in a finite time. In this paper we attempt to outline a program for physics which will meet these rigid criteria while preserving, in so far as possible, the successes that conventional physics has already achieved.

  16. Assessing the sustainable construction of large construction companies in Malaysia

    Science.gov (United States)

    Adewale, Bamgbade Jibril; Mohammed, Kamaruddeen Ahmed; Nasrun, Mohd Nawi Mohd

    2016-08-01

    Considering the increasing concerns for the consideration of sustainability issues in construction project delivery within the construction industry, this paper assesses the extent of sustainable construction among Malaysian large contractors, in order to ascertain the level of the industry's impacts on both the environment and the society. Sustainable construction explains the construction industry's responsibility to efficiently utilise the finite resources while also reducing construction impacts on both humans and the environment throughout the phases of construction. This study used proportionate stratified random sampling to conduct a field study with a sample of 172 contractors out of the 708 administered questionnaires. Data were collected from large contractors in the eleven states of peninsular Malaysia. Using the five-level rating scale (which include: 1= Very Low; 2= Low; 3= Moderate; 4= High; 5= Very High) to describe the level of sustainable construction of Malaysian contractors based on previous studies, statistical analysis reveals that environmental, social and economic sustainability of Malaysian large contractors are high.

  17. Constructability Improvement: Making Effective Use of Construction Lessons Learned

    Science.gov (United States)

    1991-01-01

    CHAPTER I - INTRODUCTION 1 CHAPTER II - FEEDBACK IN THE PROJECT LIFE-CYCLE 4 Value Engineering 4 Constructability 6 Post Occupancy Evaluations 7 CHAPTER...following section. CONCEPT/ MASTERPLAN VALUEr ENGINEERING’- DESIGN CONSTRUCTABILITY L CONSTRUCTION POST OCCUPANCY EVALUATIONS OPERATION/ p MAINTENANCE...this research is the feedback loop that begins and ends in the construction phase. Post Occupancy Evaluations Post Occupancy Evaluations (POEs) represent

  18. [Materials for construction sector].

    Science.gov (United States)

    Macchia, C

    2012-01-01

    The construction sector is characterized by high complexity due to several factors. There are a lot of processes within the building sites and they need the use of different materials with the help of appropriate technologies. Traditional materials have evolved and diversified, meanwhile new products and materials appeared and still appear, offering services which meet user needs, but that often involve risks to the health of workers. Research in the field of materials, promoted and carried out at various levels, has led to interesting results, encoded in the form of rules and laws.

  19. FAST Construction Progress

    Science.gov (United States)

    Nan, R. D.; Zhang, H. Y.; Zhang, Y.; Yang, L.; Cai, W. J.; Liu, N.; Xie, J. T.; Zhang, S. X.

    2016-11-01

    The Five-hundred-meter Aperture Spherical radio Telescope (FAST) is a Chinese mega-science project to build the largest single dish radio telescope in the world. A unique karst depression in Guizhou province has been selected as the site to build an active reflector radio telescope with a diameter of 500 m and three outstanding aspects, which enables FAST to have a large sky coverage and the ability of observing astronomical targets with a high precision. Chinese Academy of Sciences and Guizhou province are in charge of FAST construction. The first light of the telescope was expected on September 25, 2016.

  20. Constructing optimal entanglement witnesses

    Science.gov (United States)

    Chruściński, Dariusz; Pytel, Justyna; Sarbicki, Gniewomir

    2009-12-01

    We provide a class of indecomposable entanglement witnesses. In 4×4 case, it reproduces the well-known Breuer-Hall witness. We prove that these witnesses are optimal and atomic, i.e., they are able to detect the “weakest” quantum entanglement encoded into states with positive partial transposition. Equivalently, we provide a construction of indecomposable atomic maps in the algebra of 2k×2k complex matrices. It is shown that their structural physical approximations give rise to entanglement breaking channels. This result supports recent conjecture by Korbicz [Phys. Rev. A 78, 062105 (2008)].

  1. Meaning construction in remembering

    DEFF Research Database (Denmark)

    Wagoner, Brady

    2011-01-01

    F.C. Bartlett and L.S.Vygotsky were two seminal figures in the psychological study of remembering. Both emphasized the role of meaning and imagination in this process. Bartlett did this by showing the systematic and holistic changes that ensue when cultural material is repeatedly reproduced outside...... of the group to which it belongs. Vygotsky, on the other hand, attended to the ways in which we construct meaningful ―signs‖ as artificial memory aids to solve memory problems that go beyond our natural capacities. These two approaches are brought together to show how we utilize various cultural resources...

  2. Constructing Healthcare Spaces

    DEFF Research Database (Denmark)

    Harty, Chris; Holm Jacobsen, Peter; Tryggestad, Kjell

    2015-01-01

    The aim of this paper is to inquire into the role of project visualisations in shaping healthcare spaces and practices. The study draws upon an ethnographic field study from a large on-going hospital construction project in Denmark, and focuses on the early phases of on-boarding the design team...... into the project organisation. The theoretical contribution concerns the ways in which project visualisations plays an active role in developing novel conceptions of space and how these are mobilized in the process of on-boarding, in terms of 1. Design space (especially the engagement of users in the design...

  3. Constructing Regional advantage

    DEFF Research Database (Denmark)

    Asheim, Bjørn T.; Boschma, Ron; Cooke, Phil

    2011-01-01

    This paper presents a regional innovation policy model based on the idea of constructing regional advantage. This policy model brings together concepts like related variety, knowledge bases and policy platforms. Related variety attaches importance to knowledge spillovers across complementary...... economic development within and between regions in action lines appropriate to incorporate the basic principles behind related variety and differentiated knowledge bases....... sectors. The paper categorizes knowledge into ‘analytical’ (science based), ‘synthetic’ (engineering based) and ‘symbolic’ (arts based) in nature, with different requirements of ‘virtual’ and real proximity mixes. The implications of this are traced for evolving ‘platform policies’ that facilitate...

  4. Mefenamic Acid

    Science.gov (United States)

    ... any of the inactive ingredients in mefenamic acid capsules. Ask your pharmacist for a list of the inactive ingredients.tell your doctor and pharmacist what prescription and nonprescription medications, vitamins, nutritional supplements, and herbal products you are taking ...

  5. MAST Upgrade - Construction Status

    CERN Document Server

    Milnes, Joe; Dhalla, Fahim; Fishpool, Geoff; Hill, John; Katramados, Ioannis; Martin, Richard; Naylor, Graham; O'Gorman, Tom; Scannell, Rory

    2015-01-01

    The Mega Amp Spherical Tokamak (MAST) is the centre piece of the UK fusion research programme. In 2010, a MAST Upgrade programme was initiated with three primary objectives, to contribute to: 1) Testing reactor concepts (in particular exhaust solutions via a flexible divertor allowing Super-X and other extended leg configurations); 2) Adding to the knowledge base for ITER (by addressing important plasma physics questions and developing predictive models to help optimise ITER performance of ITER) and 3) Exploring the feasibility of using a spherical tokamak as the basis for a fusion Component Test Facility. With the project mid-way through its construction phase, progress will be reported on a number of the critical subsystems. This will include manufacture and assembly of the coils, armour and support structures that make up the new divertors, construction of the new set coils that make up the centre column, installation of the new power supplies for powering the divertor coils and enhanced TF coil set, progr...

  6. [Construction of high sulphite-producing industrial strain of Saccharomyces cerevisiae].

    Science.gov (United States)

    Qu, Na; He, Xiu-ping; Guo, Xue-na; Liu, Nan; Zhang, Bo-run

    2006-02-01

    In the process of beer storage and transportation, off-flavor can be produced for oxidation of beer. Sulphite is important for stabilizing the beer flavor because of its antioxidant activity. However, the low level of sulphite synthesized by the brewing yeast is not enough to stabilize beer flavor. Three enzymes involve sulphite biosynthesis in yeast. One of them, APS kinase (encoded by MET14) plays important role in the process of sulphite formation. In order to construct high sulphite-producing brewing yeast strain for beer production, MET14 gene was cloned and overexpressed in industrial strain of Saccharomyces cerevisiae. Primer 1 (5'-TGTGAATTCCTGTACACCAATGGCTACT-3', EcoR I) and primer 2 (5'-TATAAGCTTGATGA GGTGGATGAAGACG-3', HindIII) were designed according to the MET14 sequence in GenBank. A 1.1kb DNA fragment containing the open reading frame and terminator of MET14 gene was amplified from Saccharomyces cerevisiae YSF-5 by PCR, and inserted into YEp352 to generate recombinant plasmid pMET14. To express MET14 gene properly in S. cerevisiae, the recombinant expression plasmids pPM with URA3 gene as the selection marker and pCPM with URA3 gene and copper resistance gene as the selection marker for yeast transformation were constructed. In plasmid pPM, the PGK1 promoter from plasmid pVC727 was fused with the MET14 gene from pMET14, and the expression cassette was inserted into the plasmid YEp352. The dominant selection marker, copper-resistance gene expression cassette CUP1-MTI was inserted in plasmid pPM to result in pCPM. Restriction enzyme analysis showed that plasmids pPM and pCPM were constructed correctly. The laboratory strain of S. cerevisiae YS58 with ura3, trp1, leu2, his4 auxotroph was transformed with plasmid pPM. Yeast transformants were screened on synthetic minimal medium (SD) containing leucine, histidine and tryptophan. The sulphite production of the transformants carrying pPM was 2 fold of that in the control strain YS58, which showed that the

  7. Road construction in underground mines

    Energy Technology Data Exchange (ETDEWEB)

    Benke, L.; Benkovics, I.

    1985-01-01

    The need and reasons of road construction for rubber-tyre vehicles in various mine sections are examined. A detailed analysis is given of the direct and indirect influences of underground haulage ways and transport roads on the parameters of mine performance. The various mine road construction technologies are overviewed. Experiences are presented with road construction in the Mecsek Ore Mines Company, Plant 3, Hungary. The cost factors of four construction technologies are compared.

  8. Using Willie's Acid-Base Box for Blood Gas Analysis

    Science.gov (United States)

    Dietz, John R.

    2011-01-01

    In this article, the author describes a method developed by Dr. William T. Lipscomb for teaching blood gas analysis of acid-base status and provides three examples using Willie's acid-base box. Willie's acid-base box is constructed using three of the parameters of standard arterial blood gas analysis: (1) pH; (2) bicarbonate; and (3) CO[subscript…

  9. Inactivation of the ilvB1 gene in Mycobacterium tuberculosis leads to branched-chain amino acid auxotrophy and attenuation of virulence in mice.

    Science.gov (United States)

    Awasthy, Disha; Gaonkar, Sheshagiri; Shandil, R K; Yadav, Reena; Bharath, Sowmya; Marcel, Nimi; Subbulakshmi, Venkita; Sharma, Umender

    2009-09-01

    Acetohydroxyacid synthase (AHAS) is the first enzyme in the branched-chain amino acid biosynthesis pathway in bacteria. Bioinformatics analysis revealed that the Mycobacterium tuberculosis genome contains four genes (ilvB1, ilvB2, ilvG and ilvX) coding for the large catalytic subunit of AHAS, whereas only one gene (ilvN or ilvH) coding for the smaller regulatory subunit of this enzyme was found. In order to understand the physiological role of AHAS in survival of the organism in vitro and in vivo, we inactivated the ilvB1 gene of M. tuberculosis. The mutant strain was found to be auxotrophic for all of the three branched-chain amino acids (isoleucine, leucine and valine), when grown with either C(6) or C(2) carbon sources, suggesting that the ilvB1 gene product is the major AHAS in M. tuberculosis. Depletion of these branched chain amino acids in the medium led to loss of viability of the DeltailvB1 strain in vitro, resulting in a 4-log reduction in colony-forming units after 10 days. Survival kinetics of the mutant strain cultured in macrophages maintained with sub-optimal concentrations of the branched-chain amino acids did not show any loss of viability, indicating either that the intracellular environment was rich in these amino acids or that the other AHAS catalytic subunits were functional under these conditions. Furthermore, the growth kinetics of the DeltailvB1 strain in mice indicated that although this mutant strain showed defective growth in vivo, it could persist in the infected mice for a long time, and therefore could be a potential vaccine candidate.

  10. Transcriptome sequencing revealed the transcriptional organization at ribosome-mediated attenuation sites in Corynebacterium glutamicum and identified a novel attenuator involved in aromatic amino acid biosynthesis.

    Science.gov (United States)

    Neshat, Armin; Mentz, Almut; Rückert, Christian; Kalinowski, Jörn

    2014-11-20

    The Gram-positive bacterium Corynebacterium glutamicum belongs to the order Corynebacteriales and is used as a producer of amino acids at industrial scales. Due to its economic importance, gene expression and particularly the regulation of amino acid biosynthesis has been investigated extensively. Applying the high-resolution technique of transcriptome sequencing (RNA-seq), recently a vast amount of data has been generated that was used to comprehensively analyze the C. glutamicum transcriptome. By analyzing RNA-seq data from a small RNA cDNA library of C. glutamicum, short transcripts in the known transcriptional attenuators sites of the trp operon, the ilvBNC operon and the leuA gene were verified. Furthermore, whole transcriptome RNA-seq data were used to elucidate the transcriptional organization of these three amino acid biosynthesis operons. In addition, we discovered and analyzed the novel attenuator aroR, located upstream of the aroF gene (cg1129). The DAHP synthase encoded by aroF catalyzes the first step in aromatic amino acid synthesis. The AroR leader peptide contains the amino acid sequence motif F-Y-F, indicating a regulatory effect by phenylalanine and tyrosine. Analysis by real-time RT-PCR suggests that the attenuator regulates the transcription of aroF in dependence of the cellular amount of tRNA loaded with phenylalanine when comparing a phenylalanine-auxotrophic C. glutamicum mutant fed with limiting and excess amounts of a phenylalanine-containing dipeptide. Additionally, the very interesting finding was made that all analyzed attenuators are leaderless transcripts.

  11. Geotextiles in Flexible Pavement Construction

    Science.gov (United States)

    Alungbe, Gabriel D.

    2004-01-01

    People everywhere in the developed world regularly drive on paved roads. Learning about the construction techniques and materials used in paving benefits technology and construction students. This article discusses the use of geosynthetic textiles in pavement construction. It presents background on pavements and describes geotextiles and drainage…

  12. Construction of optimized Boolean functions

    Institute of Scientific and Technical Information of China (English)

    CHEN Wei; YANG Yi-xian; NIU Xin-xin

    2006-01-01

    Considering connections of characteristics,this paper is aimed at the construction of optimized Boolean functions.A new method based on the Bent function,discrete Walsh spectrum and characteristics matrices are presented by concatenating,breaking,and revising output sequences conditionally.This new construction can be used to construct different kinds of functions satisfying different design criteria.

  13. Inductive types in constructive languages

    NARCIS (Netherlands)

    Bruin, Peter Johan de

    1995-01-01

    This dissertation deals with constructive languages: languages for the formal expression of mathematical constructions. The concept of construction does not only encompass computations, as expressed in programming languages,but also propositions and proofs, as expressed in a mathematical logic, and

  14. SPEAR3 Construction Alignment

    Energy Technology Data Exchange (ETDEWEB)

    LeCocq, Catherine; Banuelos, Cristobal; Fuss, Brian; Gaudreault, Francis; Gaydosh, Michael; Griffin, Levirt; Imfeld, Hans; McDougal, John; Perry, Michael; Rogers,; /SLAC

    2005-08-17

    An ambitious seven month shutdown of the existing SPEAR2 synchrotron radiation facility was successfully completed in March 2004 when the first synchrotron light was observed in the new SPEAR3 ring, SPEAR3 completely replaced SPEAR2 with new components aligned on a new highly-flat concrete floor. Devices such as magnets and vacuum chambers had to be fiducialized and later aligned on girder rafts that were then placed into the ring over pre-aligned support plates. Key to the success of aligning this new ring was to ensure that the new beam orbit matched the old SPEAR2 orbit so that existing experimental beamlines would not have to be reoriented. In this presentation a pictorial summary of the Alignment Engineering Group's surveying tasks for the construction of the SPEAR3 ring is provided. Details on the networking and analysis of various surveys throughout the project can be found in the accompanying paper.

  15. Constructing Healthcare Spaces

    DEFF Research Database (Denmark)

    Harty, Chris; Holm Jacobsen, Peter; Tryggestad, Kjell

    2015-01-01

    into the project organisation. The theoretical contribution concerns the ways in which project visualisations plays an active role in developing novel conceptions of space and how these are mobilized in the process of on-boarding, in terms of 1. Design space (especially the engagement of users in the design...... process), 2.Organisational space (work processes and their spatial-temporal dimension) and; 3. Economic space (cost estimations and budgets). In practice, our findings show that the visualisations of different yet connected project spaces and the development of future clinical practices is related......The aim of this paper is to inquire into the role of project visualisations in shaping healthcare spaces and practices. The study draws upon an ethnographic field study from a large on-going hospital construction project in Denmark, and focuses on the early phases of on-boarding the design team...

  16. Complex performance in construction

    DEFF Research Database (Denmark)

    Bougrain, Frédéric; Forman, Marianne; Gottlieb, Stefan Christoffer

    To fulfil the expectations of demanding clients, new project-delivery mechanisms have been developed. Approaches focusing on performance-based building or new procurement processers such as new forms of private-public partnerships are considered as solutions improving the overall performance...... of the industry. The main objective of this project was to understand how the development of integrated solutions in construction led to distinct configuration of actors and structures. Furthermore, the project analyses whether these changes modified project processes and contributed to the delivery of new value...... to the end users. This report summarises the results from work undertaken in the international collaborative project “Procuring and Operating Complex Products and Systems in Construction” (POCOPSC). POCOPSC was carried out in the period 2010-2014. The project was executed in collaboration between CSTB...

  17. Constructing visual representations

    DEFF Research Database (Denmark)

    Huron, Samuel; Jansen, Yvonne; Carpendale, Sheelagh

    2014-01-01

    The accessibility of infovis authoring tools to a wide audience has been identified as a major research challenge. A key task in the authoring process is the development of visual mappings. While the infovis community has long been deeply interested in finding effective visual mappings......, comparatively little attention has been placed on how people construct visual mappings. In this paper, we present the results of a study designed to shed light on how people transform data into visual representations. We asked people to create, update and explain their own information visualizations using only...... tangible building blocks. We learned that all participants, most of whom had little experience in visualization authoring, were readily able to create and talk about their own visualizations. Based on our observations, we discuss participants’ actions during the development of their visual representations...

  18. TOTEM Construction Complete

    CERN Multimedia

    Katarina Anthony

    2011-01-01

    The winter technical stop saw the final steps of the installation of the TOTEM experiment. After 8 years of development, the inelastic telescope T1 was successfully installed inside CMS at both sides of the interaction point. This detector joins the previously installed inelastic telescope T2 and the Roman Pots (147m and 220m from the CMS interaction point) to study both elastic and inelastic proton scattering.   The Genova team who designed and built the detector in front of the '+' arm of the T1 telescope after final insertion. All the detector chambers were assembled at the Petersburg Nuclear Physics Institute, Gatchina, Russia. Major contributions to the construction of the support structure and the installation process came from CERN. “Installing the T1 detector was an important milestone for TOTEM,” says Joachim Baechler, Technical Coordinator for TOTEM. Enrico Robutti from Genova, responsible for the T1 project, added: "The T1 detector was carefully installed ...

  19. Environmentally friendly road construction

    Directory of Open Access Journals (Sweden)

    A.I. Essawy

    2013-06-01

    Full Text Available Environmental pollution is a major problem in developing countries like Egypt. Reuse of waste polymers is considered an attractive solution for environmental white pollution and reducing of the costs of road pavement and maintenance. This research aims to prepare environmentally friendly hot mix asphalt (HMA for paving using some industrial wastes as polypropylene and polyester fibers. The solid materials in the mix include normal and highly porous aggregates. 5% and 10% of waste polymers by weight of the asphalt were used to prepare special binders. The samples were tested for their physical properties, chemical properties, aging, scanning electron microscopy (SEM and thermo-gravimetric analysis (TGA. The results revealed that the prepared HMA using 5% of waste polymer had high performance as compared to the ordinary one and the waste polymer could be used in road construction.

  20. Constructing Data Curation Profiles

    Directory of Open Access Journals (Sweden)

    Michael Witt

    2009-12-01

    Full Text Available This paper presents a brief literature review and then introduces the methods, design, and construction of the Data Curation Profile, an instrument that can be used to provide detailed information on particular data forms that might be curated by an academic library. These data forms are presented in the context of the related sub-disciplinary research area, and they provide the flow of the research process from which these data are generated. The profiles also represent the needs for data curation from the perspective of the data producers, using their own language. As such, they support the exploration of data curation across different research domains in real and practical terms. With the sponsorship of the Institute of Museum and Library Services, investigators from Purdue University and the University of Illinois interviewed 19 faculty subjects to identify needs for discovery, access, preservation, and reuse of their research data. For each subject, a profile was constructed that includes information about his or her general research, data forms and stages, value of data, data ingest, intellectual property, organization and description of data, tools, interoperability, impact and prestige, data management, and preservation. Each profile also presents a specific dataset supplied by the subject to serve as a concrete example. The Data Curation Profiles are being published to a public wiki for questions and discussion, and a blank template will be disseminated with guidelines for others to create and share their own profiles. This study was conducted primarily from the viewpoint of librarians interacting with faculty researchers; however, it is expected that these findings will complement a wide variety of data curation research and practice outside of librarianship and the university environment.

  1. Two Constructions of Libertarianism

    Directory of Open Access Journals (Sweden)

    Chandran Kukathas

    2009-02-01

    Full Text Available The libertarian first principle—a belief in individual freedom—can lead to two different and not necessarily acceptable societies from the standpoint of liberty. One is the “Union of Liberty,” in which communities, associations, and intermediate bodies are held to rigorous standards of voluntariness (and thus face sharp limits on their internal associational freedom because of the knowledge that children will be born into them. In the other, the “Federation of Liberty,” they are not (thereby allowing children to be born into locally unfree environments.While in any free society individuals may voluntarily join together and waive some of their rights (in institutions such as contract or marriage, for example, hard questions arise when nonconsenting children are born into restrictive environments that their parents may have voluntarily created. An adult who gives up all of his or her property to a communal religious body upon conversion has made a voluntary choice, but what about the child born into that religious community later on? Thus, the Federation of Liberty can, in theory, turn out to contain no communities that actually value or respect liberty; and even slavery might have a lawful place within it. The Union of Liberty, on the other hand, can, in principle turn out to be society ruled by a strong authority with little respect for dissenting moral traditions, including some self-styled libertarian moral traditions.Libertarians face a stark choice between these “two constructions of libertarianism”; there is no third way, theoretically speaking. Libertarians must choose one of them. Given the necessity to choose one of these constructions, the Federation of Liberty is arguably preferable to the Union of Liberty.

  2. Construction and Analysis of N-phosphoryl Peptide Libraries

    Institute of Scientific and Technical Information of China (English)

    Shu Xia CAO; Jian Chen ZHANG; Ming Yu NIU; Kui LU; Xin Cheng LIAO; Yu Fen ZHAO

    2004-01-01

    N-Phosphoryl peptide libraries were constructed by transformation from homo-oligopeptide libraries, which was synthesized by self-assembly of amino acids with the assistance of phosphorus oxychloride. Electrospray ionization mass spectrometry (ESI-MS) was used to monitor the reaction.

  3. A New Application of Carbon Nanotubes Constructing Biosensor

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Carbon nanotubes used for constructing biosensor was described for the first time. Single-wall carbon nanotubes (SWNTs) functionalized with carboxylic acid groups were used to immobilize glucose oxidase forming a glucose biosensor. The biosensor response can be determined by amperometric method at a low applied potential (0.40 V).

  4. 鼠伤寒沙门菌spvB基因缺陷变异株的制备及其抗酸能力检测%Construction of a spvB Gene-deleted Mutant of Salmonella Enterica Serovar Typhi and Its Survival Ability in Acid Condition

    Institute of Scientific and Technical Information of China (English)

    吴春雪; 陈强; 李红; 余晓君; 朱春晖; 李岚; 何美娟; 刘晓艳

    2014-01-01

    Objective To investigate the function of spvB gene,a toxicity gene of Salmonella enterica serovar Typhi,by constructing the spvB gene-deleted mutant and examinimg its survival ability in acid condition.Methods According to the se-quences of spvB gene of Salmonella enterica serovar Typhi,specific primers were designed for PCR.The homologous DNA fragments with spvB gene deleted were constructed,which was cloned into the suicide plasmid pCVD442 and then transferred into the target cells of Salmonella enterica serovar Typhi.The recombination was visualized by PCR,and the complete recombi-nant strain was selected as the spvB gene-deleted mutant strain and confirmed by the corresponding sequencing analysis.Under the acid condition,the survival ability of the spvB mutant and parent was compared by using the growth curve.Results A dele-tion of 1 748 bp of the spvB gene was confirmed by PCR and sequencing analysis in spvB gene-deleted mutants.The number of live wild-type strains were significantly greater than that of spvB gene-deleted mutants under the acid condition for 1 and 2 h, and the difference was statistically significant(P<0.05).The survival rate of the wild-type strains was 85.6% and 74.9% at 1 and 2 h,significantly higher than that of the spvB gene-deleted mutants,which was 68.0% and 42.3%.Conclusion The spvB gene-deleted mutant of Salmonella enterica serovar Typhi was successfully generated and its survival ability was significantly compromised under the acid condition,which lays a foundation for studying the function of the spvB gene in Salmonella enteri-ca serovar Typhi.%目的:为深入研究鼠伤寒沙门菌毒力基因 spvB的功能,制备鼠伤寒沙门菌 spvB基因完全缺陷变异株,观察spvB基因缺陷株在体外酸性环境中的生存能力。方法根据鼠伤寒沙门菌spvB基因序列,设计PCR特异性引物,制备spvB基因缺陷性同源性核苷酸片段,导入自杀质粒 pCVD442后再导入鼠伤寒沙门菌野生

  5. Inspection of Construction Works According to Polish Construction Law

    Science.gov (United States)

    Czemplik, A.

    2015-11-01

    Construction regulations are still different in many European countries, even though the European Union directives have unified many acts for construction works and construction products in the member countries. The scheme of the construction process presented in the paper could be valid for most countries, despite of detailed regulations of legal systems. The number of construction regulations to be followed in order to get the Construction Permit in Poland is rather big, so the time between the start of the investment process and the day when the Construction Permit is issued could be several months. Only licensed professional engineers can play the role of site managers, site inspectors and designers, registered for the given construction project. Duties and responsibilities (civil liability) of these engineers are strictly defined by regulations. The obligatory inspection of construction works should be executed by the licensed site inspectors. Moreover, the works can be incidentally inspected by Authority, banks, insurance companies or designers. Foreign designers and foreign site engineers in order to be allowed by respective Authority to play official roles on Polish construction sites should present documents proving that they can do the same jobs in their countries as per regulations obligatory there.

  6. Acid Rain

    Science.gov (United States)

    Bricker, Owen P.; Rice, Karen C.; Dietrich, W.E.; Sposito, Garrison

    1997-01-01

    Acid deposition, or acid rain as it is more commonly referred to, has become a widely publicized environmental issue in the U.S. over the past decade. The term usually conjures up images of fish kills, dying forests, "dead" lakes, and damage to monuments and other historic artifacts. The primary cause of acid deposition is emission of S02 and NOx to the atmosphere during the combustion of fossil fuels. Oxidation of these compounds in the atmosphere forms strong acids - H2SO4 and HNO3 - which are returned to the Earth in rain, snow, fog, cloud water, and as dry deposition.Although acid deposition has only recently been recognized as an environmental problem in the U.S., it is not a new phenomenon (Cogbill & Likens 1974). As early as the middle of the 17th century in England, the deleterious effects of industrial emissions on plants, animals, and humans, and the atmospheric transport of pollutants between England and France had become issues of concern (Evelyn 1661, Graunt 1662). It is interesting that well over three hundred years ago in England, recommendations were made to move industry outside of towns and build higher chimneys to spread the pollution into "distant parts." Increasing the height of smokestacks has helped alleviate local problems, but has exacerbated others. In the U.S. the height of the tallest smokestack has more than doubled, and the average height of smokestacks has tripled since the 1950s (Patrick et al 1981). This trend occurred in most industrialized nations during the 20th century and has had the effect of transforming acid rain from a local urban problem into a problem of global scale.

  7. Carbonic Acid Pretreatment of Biomass

    Energy Technology Data Exchange (ETDEWEB)

    G. Peter van Walsum; Kemantha Jayawardhana; Damon Yourchisin; Robert McWilliams; Vanessa Castleberry

    2003-05-31

    acid compared to water alone. 6) Determine optimal conditions for carbonic acid pretreatment of aspen wood. Optimal severities appeared to be in the mid range tested. ASPEN-Plus modeling and economic analysis of the process indicate that the process could be cost competitive with sulfuric acid if the concentration of solids in the pretreatment is maintained very high (~50%). Lower solids concentrations result in larger reactors that become expensive to construct for high pressure applications.

  8. Carbonic Acid Retreatment of Biomass

    Energy Technology Data Exchange (ETDEWEB)

    Baylor university

    2003-06-01

    the use of carbonic acid compared to water alone. (6) Determine optimal conditions for carbonic acid pretreatment of aspen wood. Optimal severities appeared to be in the mid range tested. ASPEN-Plus modeling and economic analysis of the process indicate that the process could be cost competitive with sulfuric acid if the concentration of solids in the pretreatment is maintained very high ({approx}50%). Lower solids concentrations result in larger reactors that become expensive to construct for high pressure applications.

  9. Lewis acid promoted highly diastereoselective Petasis Borono-Mannich reaction: efficient synthesis of optically active β,γ-unsaturated α-amino acids.

    Science.gov (United States)

    Li, Yi; Xu, Ming-Hua

    2012-04-20

    An efficient and straightforward method for the preparation of highly enantiomerically enriched β,γ-unsaturated α-amino acid derivatives by a Lewis acid promoted diastereoselective Petasis reaction of vinylboronic acid, N-tert-butanesulfinamide, and glyoxylic acid has been developed. The synthetic utilities of the approach were demonstrated by the rapid and convenient construction of challenging cyclopenta[c]proline derivatives.

  10. Exploration of structure-function relationships in Escherichia coli cystathionine γ-synthase and cystathionine β-lyase via chimeric constructs and site-specific substitutions.

    Science.gov (United States)

    Manders, Adrienne L; Jaworski, Allison F; Ahmed, Mohammed; Aitken, Susan M

    2013-06-01

    Cystathionine γ-synthase (CGS) and cystathionine β-lyase (CBL) share a common structure and several active-site residues, but catalyze distinct side-chain rearrangements in the two-step transsulfuration pathway that converts cysteine to homocysteine, the precursor of methionine. A series of 12 chimeric variants of Escherichia coli CGS (eCGS) and CBL (eCBL) was constructed to probe the roles of two structurally distinct, ~25-residue segments situated in proximity to the amino and carboxy termini and located at the entrance of the active-site. In vivo complementation of methionine-auxotrophic E. coli strains, lacking the genes encoding eCGS and eCBL, demonstrated that exchange of the targeted regions impairs the activity of the resulting enzymes, but does not produce a corresponding interchange of reaction specificity. In keeping with the in vivo results, the catalytic efficiency of the native reactions is reduced by at least 95-fold, and α,β versus α,γ-elimination specificity is not modified. The midpoint of thermal denaturation monitored by circular dichroism, ranges between 59 and 80°C, compared to 66°C for the two wild-type enzymes, indicating that the chimeric enzymes adopt a stable folded structure and that the observed reductions in catalytic efficiency are due to reorganization of the active site. Alanine-substitution variants of residues S32 and S33, as well as K42 of eCBL, situated in proximity to and within, respectively, the targeted amino-terminal region were also investigated to explore their role as determinants of reaction specificity via positioning of key active-site residues. The catalytic efficiency of the S32A, S33A and the K42A site-directed variants of eCBL is reduced by less than 10-fold, demonstrating that, while these residues may participate in positioning S339, which tethers the catalytic base, their role is minor.

  11. Sustainable regulation of construction.

    Science.gov (United States)

    2000-11-01

    The seminar examined the role building codes and regulations can have in promoting a more sustainable approach to construction, particularly through their application to non-industrial building materials. A range of building materials such as straw, bamboo, rammed earth, adobe, and cob (a mixture of clay and chopped straw) were described and illustrated by slides to show their building potential. The current codes have a prime concern to protect the health and safety of people from the built environment. They have been developed almost exclusively for mainstream industrial materials and methods of construction, which makes them difficult to use with alternative, indigenous, or non-industrial building materials, even though those materials may be considered more sustainable. The argument was put forward that with only one-third of the world population living in modern industrial buildings today, it is not sustainable to re-house the remaining rapidly expanding population in high technology dwellings. Many of the low technology building materials and methods now used by the majority of people in the world need only incremental improvement to be equal or superior to many of their industrial replacements. Since these can be more sustainable methods of building, there needs to be an acceptance of the use of alternative materials, particularly in the developing parts of the world, where they are being rejected for less sustainable industrial methods. However, many codes make it difficult to use non-industrial materials; indeed, many of the industrial materials would not meet the demands that must be now met if they were now being introduced as new materials. Consequently, there is a need to develop codes to facilitate the use of a wider range of materials than in current use, and research is needed to assist this development. Sustainable regulation should take into account the full range of real impacts that materials and systems have in areas such as resource use and

  12. Trypanocidal Effect of Isotretinoin through the Inhibition of Polyamine and Amino Acid Transporters in Trypanosoma cruzi

    Science.gov (United States)

    Reigada, Chantal; Valera-Vera, Edward A.; Sayé, Melisa; Errasti, Andrea E.; Avila, Carla C.; Miranda, Mariana R.; Pereira, Claudio A.

    2017-01-01

    Polyamines are essential compounds to all living organisms and in the specific case of Trypanosoma cruzi, the causative agent of Chagas disease, they are exclusively obtained through transport processes since this parasite is auxotrophic for polyamines. Previous works reported that retinol acetate inhibits Leishmania growth and decreases its intracellular polyamine concentration. The present work describes a combined strategy of drug repositioning by virtual screening followed by in vitro assays to find drugs able to inhibit TcPAT12, the only polyamine transporter described in T. cruzi. After a screening of 3000 FDA-approved drugs, 7 retinoids with medical use were retrieved and used for molecular docking assays with TcPAT12. From the docked molecules, isotretinoin, a well-known drug used for acne treatment, showed the best interaction score with TcPAT12 and was selected for further in vitro studies. Isotretinoin inhibited the polyamine transport, as well as other amino acid transporters from the same protein family (TcAAAP), with calculated IC50 values in the range of 4.6–10.3 μM. It also showed a strong inhibition of trypomastigote burst from infected cells, with calculated IC50 of 130 nM (SI = 920) being significantly less effective on the epimastigote stage (IC50 = 30.6 μM). The effect of isotretinoin on the parasites plasma membrane permeability and on mammalian cell viability was tested, and no change was observed. Autophagosomes and apoptotic bodies were detected as part of the mechanisms of isotretinoin-induced death indicating that the inhibition of transporters by isotretinoin causes nutrient starvation that triggers autophagic and apoptotic processes. In conclusion, isotretinoin is a promising trypanocidal drug since it is a multi-target inhibitor of essential metabolites transporters, in addition to being an FDA-approved drug largely used in humans, which could reduce significantly the requirements for its possible application in the treatment of

  13. Towards Reassignment of the Methionine Codon AUG to Two Different Noncanonical Amino Acids in Bacterial Translation

    Directory of Open Access Journals (Sweden)

    Alessandro De Simone

    2016-06-01

    Full Text Available Genetic encoding of noncanonical amino acids (ncAAs through sense codon reassignment is an efficient tool for expanding the chemical functionality of proteins. Incorporation of multiple ncAAs, however, is particularly challenging. This work describes the first attempts to reassign the sense methionine (Met codon AUG to two different ncAAs in bacterial protein translation. Escherichia coli methionyl-tRNA synthetase (MetRS charges two tRNAs with Met: tRNAfMet initiates protein synthesis (starting AUG codon, whereas elongator tRNAMet participates in protein elongation (internal AUG codon(s. Preliminary in vitro experiments show that these tRNAs can be charged with the Met analogues azidohomoalanine (Aha and ethionine (Eth by exploiting the different substrate specificities of EcMetRS and the heterologous MetRS / tRNAMet pair from the archaeon Sulfolobus acidocaldarius, respectively. Here, we explored whether this configuration would allow a differential decoding during in vivo protein initiation and elongation. First, we eliminated the elongator tRNAMet from a methionine auxotrophic E. coli strain, which was then equipped with a rescue plasmid harboring the heterologous pair. Although the imported pair was not fully orthogonal, it was possible to incorporate preferentially Eth at internal AUG codons in a model protein, suggesting that in vivo AUG codon reassignment is possible. To achieve full orthogonality during elongation, we imported the known orthogonal pair of Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS / tRNAPyl and devised a genetic selection system based on the suppression of an amber stop codon in an important glycolytic gene, pfkA, which restores enzyme functionality and normal cellular growth. Using an evolved PylRS able to accept Met analogues, it should be possible to reassign the AUG codon to two different ncAAs by using directed evolution. This work is licensed under a Creative Commons Attribution 4.0 International

  14. Constructing Sentient Androids

    Institute of Scientific and Technical Information of China (English)

    AMOROSO; Richard; L.

    2015-01-01

    Generally the simplistic distinction between a humanoid robot,a computerized machine capable of replicating a variety of complex human functions automatically,and an android is one of appearance; an android is meant to look and act like a human being even to the extent of being indistinguishable. While one day a yottaflop(1024 bits per second) hyper-supercomputer could have a sufficient holographic database and processing power to be truly indistinguishable from a human being,the issue of the applicability of sentience(self-awareness) to an android comes to the forefront. The currently dominant cognitive model of awareness,closely aligned to the AI model,states that mind equals brain and that once correct algorithms are known all of human intelligence could be replicated artificially. This is the so-called mechanistic view: ‘The laws of physics and chemistry are sufficient to describe all living systems; no additional life principle is required’. In this work we develop the point of view that the regime of Unified Field Mechanics(UFM) supplies an inherent action principle driving both the evolution of complex Self-Organized Living Systems(SOLS) and the physical processes of awareness. These UFM parameters in conjunction with ‘conscious quantum computing’(class of quantum computer modeled with physical parameters of mind-body interaction) putatively leads directly to the construction of sentient(or sentient-like) Androids.

  15. Culture in constructive remembering

    DEFF Research Database (Denmark)

    Wagoner, Brady

    2012-01-01

    The present chapter explores novel ways of thinking about what it means to remember and how precisely culture is involved in this process. Since Plato, the dominant metaphor for conceptualizing memory has been that of a spatial ‘storage’. In contrast to this, Frederic Bartlett advanced an alterna......The present chapter explores novel ways of thinking about what it means to remember and how precisely culture is involved in this process. Since Plato, the dominant metaphor for conceptualizing memory has been that of a spatial ‘storage’. In contrast to this, Frederic Bartlett advanced...... an alternative temporal metaphor of remembering as ‘construction’. If we push his metaphor further—with the help of cultural psychology—we can say memory construction is done by agents using cultural ‘tools’ such as language and narrative. In this chapter, Bartlett’s theory is contextualized, elucidated......, critiqued and developed with the help of a number of other thinkers. The ultimate aim of the chapter is to go beyond Bartlett and arrive at a thoroughgoing culturally inclusive psychological theory of remembering. Though Bartlett clearly situated remembering within a social process, he did not provide...

  16. Quantitative proteomics by amino acid labeling identifies novel NHR-49 regulated proteins in C. elegans

    DEFF Research Database (Denmark)

    Fredens, Julius; Færgeman, Nils J.

    2012-01-01

    in the nematode Caenorhabditis elegans. We have recently shown that C. elegans can be completely labeled with heavy-labeled lysine by feeding worms on prelabeled lysine auxotroph Escherichia coli for just one generation. We applied this methodology to examine the organismal response to functional loss or RNAi...... gene knockdown by RNAi provides a powerful tool with broad implications for C. elegans biology....

  17. Biomedical problems of hydrotechnical construction

    Energy Technology Data Exchange (ETDEWEB)

    Avakyan, A.B.; El' piner, L.I.; Delitsyn, V.M.

    1988-04-01

    The effect of hydrotechnical and water-management construction on the living conditions and health of the population was examined. The results were used to develop the scientific bases and methods of biomedical predictions in several stages: evaluating biomedical conditions in territories where a change is expected, and constructing biomedical prediction proper of the effect of hydrotechnical constructions. The development of the indicated predictions make it possible to include measures on intensifying the positive and preventing or abating undesired effects on the biomedical situation when designing hydrotechnical and water-management construction.

  18. Construction of Pseudomonas sp.M18 relA mutant and its regulation on phenazine-1-carboxylic acid biosynthesis%假单胞菌M18relA突变株的构建及其对吩嗪-1-羧酸合成的调控

    Institute of Scientific and Technical Information of China (English)

    王金英; 张明月; 王国昊; 魏雪; 李雅乾; 黄显清; 许煜泉

    2011-01-01

    The rhizosphere bacterium Pseudomonas sp. M 18 can simultaneously produce two antibiotics:phenazine-1- carboxylic acid (PCA) and pyoluteorin. ppGpp, which is synthesized by Re1A, can mediate bacterial stringent response to nutritional starvation. The relA gene was PCR amplified from the M18 strain chromosomal DNA template. The relA mutant of M18 strain (M18RAG) was constructed through inserted inactivation of gentamicin resistance cassette and homologous recombination. PCA production was assayed in PPM media. It was showed that the relA mutation resulted in a significant enhancement of PCA production. PCA production of M 18RAG was about 1.5 to 2 times as much as that of the wild-type strain. The negative regulation of RelA on PCA biosynthesis and its gene expression was further confirmed by the trans complementation test of relA gene and the expression analysis of phzA '-'lacZ translational fusion.%假单胞菌M18是一株能同时合成吩嗪-1-羧酸(PCA)和藤黄绿菌素两种抗生素的植物根际分离细菌.RelA催化合成的效应分子ppGpp能介导细菌因营养饥饿引起的应激反应.以M18菌株染色体DNA为模板,PCR扩增获得relA基因,通过庆大霉素抗性片段插入失活与同源重组技术,构建假单胞菌M18的relA突变菌株M18RAG.在PPM培养基中进行PCA发酵分析,发现突变菌株M18RAG的PCA产量显著升高,约为野生型菌株的1.5-2倍.relA基因反式互补实验以及phzA'-'lacZ翻译融合测定结果,均进一步证明了RelA对PCA生物合成及其基因表达具有抑制作用.

  19. Artificial vessel construction in nude mice by subcutaneous implantation of polyglicolide acid cocultured with vascular endothelial cells and smooth muscle cells%血管内皮细胞和平滑肌细胞-聚羟基乙酸复合物置入裸鼠皮下构建人工血管

    Institute of Scientific and Technical Information of China (English)

    刘阳; 张燕中; 陈瑾君; 殷德民; 曹颖; 许志成; 刘伟; 崔磊; 曹谊林

    2008-01-01

    BACKGROUND: A lot of researches have proved that polyglicolide acid (PGA), as a stent material, has been successfully used to construct engineered tissues, such as cartilage, bone and tendons, in nude mice or even big mammal. Whether the incubation of vascular endothelial cells and smooth muscle cells in the polyglicolide acid may subcutaneously construct vessel-like structure in nude mice needs a further study.OBJECTIVE: To verify the feasibility of forming vessel-like structure in the nude mice by subcutaneous implantation of polyglicolide acid cocultured with vascular endothelial cells and smooth muscle cells derived from newborn umbilical vein.DESIGN: Contrast study.SETTING: Tissue Engineering Key Laboratory, Medical College, Shanghai Jiao Tong University.MATERIALS: This study was performed at Tissue Engineering Key Laboratory, Medical College, Shanghai Jiao Tong University from January to June 2002. Belly band was derived from newborn babies in our department of obstetrics & gynecology. The parturien provided the informed consent, and this study was approved by the local research ethics committee. Twenty-six nude mice (3-4 weeks old, clean grade, irrespective of gender) were selected in this study. The animal experiment received confirmed consent from the local ethic committee. Polyglicolide acid was provided by Albany International Research Co.METHODS: Vascular endothelial cells and smooth muscle cells derived from newborn umbilical vein were incubated on a piece of polyglicolide acid to produce cell-material compound. In addition, the compound covered around the silicone tube to form a tube-like structural substance. Subsequently, the tube-like structural substance was subcutaneously implanted in 20 nude mice, which were regarded as an experimental group. And then, polyglicolide acid alone was subcutaneously transplanted in the rest 6 nude mice, which was regarded as a control group.MAIN OUTCOME MEASURES: Gross observations of cell-material compound by 2 and

  20. Conformational classification of functional nucleic acids.

    Science.gov (United States)

    Fujii, S

    1999-01-01

    The kink parameters would provide the tolerant aspect for irregular helical structure of nucleic acid. Using these kink parameters, the classification of conformation space was carried for the functional nucleic acid molecules. The kink parameters could afford us the simple structural aspects about the constructive parts of functional molecules. Local elastic kink phenomena can be classified by rod like models with the combination of kink parameters. The constructive parts, such as the stable tetra nucleotides loop, U-turn conformation and adenosine platform, were selected and the statistical analyses were carried on the parameters calculated by program BIOCON.

  1. Hydroxycarboxylic acids and salts

    Energy Technology Data Exchange (ETDEWEB)

    Kiely, Donald E; Hash, Kirk R; Kramer-Presta, Kylie; Smith, Tyler N

    2015-02-24

    Compositions which inhibit corrosion and alter the physical properties of concrete (admixtures) are prepared from salt mixtures of hydroxycarboxylic acids, carboxylic acids, and nitric acid. The salt mixtures are prepared by neutralizing acid product mixtures from the oxidation of polyols using nitric acid and oxygen as the oxidizing agents. Nitric acid is removed from the hydroxycarboxylic acids by evaporation and diffusion dialysis.

  2. Evaluation as Construction of Knowledge.

    Science.gov (United States)

    Kvale, Steinar

    Evaluation is discussed as knowledge construction. Examinations are considered as tests of knowledge or as tests of students and then as selection tools or as the construction of knowledge in themselves. Examinations involve the power to define what is common knowledge in a discipline and in a culture, and the examination discourse contributes to…

  3. Strategic Scenario Construction Made Easy

    DEFF Research Database (Denmark)

    Duus, Henrik Johannsen

    2016-01-01

    insights from the area of strategic forecasting (of which scenario planning is a proper subset) and experiences gained from a recent course in that area to develop a simpler, more direct, hands-on method for scenario construction and to provide several ideas for scenario construction that can be used...

  4. Toward Constructing the Therapeutic System.

    Science.gov (United States)

    Andolfi, Maurizio; Angelo, Claudio

    1988-01-01

    Describes the therapist as an active participant in the construction of the therapeutic system, explaining how the therapist constructs complex relationships within the evolving therapeutic process. Reevaluates the importance of the individual in the family as an agent of change and as a mediator of triangular relational messages. (Author/NB)

  5. Constructing optimal entanglement witnesses. II

    CERN Document Server

    Chruscinski, Dariusz

    2010-01-01

    We provide a class of optimal nondecomposable entanglement witnesses for 4N x 4N composite quantum systems or, equivalently, a new construction of nondecomposable positive maps in the algebra of 4N x 4N complex matrices. This construction provides natural generalization of the Robertson map. It is shown that their structural physical approximations give rise to entanglement breaking channels.

  6. Teacher Guidance of Knowledge Construction

    Science.gov (United States)

    Schwarz, Baruch; Dreyfus, Tommy; Hadas, Nurit; Hershkowitz, Rina

    2004-01-01

    This paper focuses on how teachers guide construction of knowledge in classrooms. We suggest that guidance hinges on the kind of dialogue teachers choose to engage students in. We propose several classroom dialogue types relevant for the construction of knowledge and suggest that critical dialogue is particularly effective for knowledge…

  7. Constructing higher dimensional local fields

    CERN Document Server

    Morrow, Matthew

    2012-01-01

    This note is a gentle introduction to higher dimensional local fields, with the motivating problem being the standard geometric "localisation-completion" process by which they can be constructed. A direct proof of the behaviour of this construction, which is the simplest part of the theory of higher dimensional adeles, will hopefully be useful to both specialists and newcomers.

  8. Design Processes and Constructive Ergonomics

    DEFF Research Database (Denmark)

    Broberg, Ole

    2015-01-01

    This paper discusses some central issues in the concept of constructive ergonomics. It does so by reflecting on experiences from ergonomics intervention projects carried out in Denmark. Constructive ergonomics has a huge potential as a new way to go for ergonomics research and practice. However, ...

  9. Language Test Construction and Evaluation.

    Science.gov (United States)

    Alderson, J. Charles; And Others

    The guide is intended for teachers who must construct language tests and for other professionals who may need to construct, evaluate, or use the results of language tests. Most examples are drawn from the field of English-as-a-Second-Language instruction in the United Kingdom, but the principles and practices described may be applied to the…

  10. New Ideas for School Construction.

    Science.gov (United States)

    Producers' Council, Inc., Washington, DC.

    Present educators, architects, engineers, and building product manufacturers with a medium of common interest for discussion of mutual school construction problems, objectives, needs, ideas, capabilities and limitations. Contents include--(1) modern wood construction, (2) school room in a steel mill, (3) masonry in new school design, (4) the…

  11. Constructions over localizations of rings

    Directory of Open Access Journals (Sweden)

    Alessandro Logar

    1987-11-01

    Full Text Available In this paper we construct a category of effective noetherian rings in which linear equations can be “solved”. This category is closed with respect to some important constructions like trascendental extensions, quotientations, finite products and localizations with respect to a large class of multiplicatively closed systems. Hence it gives a definition of “constructive” rings.

  12. About Hebei Jianxin Construction Group

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Hebei Jianxin Construction (Group) Ltd. was incorporated in August 1984. As the holding company, Hebei Jianxin Construction (Group)Ltd. along with five companies under its holding, such as Baoding New Generation Real Estate Development Co., Ltd. and Baoding New Generation Property Management Co., Ltd., formed Hebei Jianxin Architectural Group (hereafter, the Group).

  13. Workshop presentation: research guidelines for Construction Management

    Directory of Open Access Journals (Sweden)

    Marco Alvise Bragadin

    2013-10-01

    Full Text Available Nowadays the European economic system challenges the construction sector to take part to industrial recovery of western countries. In co-operation with the Construction Production research group of the Tampere University of of research about construction management tools and methods were detected. Research guidelines: 1 Construction management: tools and methods to manage construction projects 2 environmental impact of construction projects 3 construction management and safety 4 project procurement 5 construction management for major public works & complex projects

  14. Innovations in Construction Personnel Education

    Directory of Open Access Journals (Sweden)

    Nicał Aleksander

    2016-01-01

    Full Text Available The Erasmus+ programme, financed by the European Union, encourages international projects focused on education and recognition of professional qualifications. Projects presented in this paper deepen the ideas of Directive 89/48/EWG, which will lead to the creation of a proper European system of comparison, certification and mutual recognition of qualifications in construction. Results of the projects will improve didactic process and qualifications recognition of European Union managers in construction. Paper presents 3 Erasmus+ projects: “The Augmented Reality Formwork Assembly Training” (Project number 2016-1-PL01-KA202-026102, “Common Learning Outcomes for Construction Managers in EU, part IV” (Project number 2015-1-PL01-KA202-016454, and “Continuing V.E.T. Training Programme in Lean Construction to support the transition of the Construction Sector towards a more competitive and productive model” (Project number 2016-1-ES01-KA202-025694.

  15. CRITICAL EVENTS IN CONSTRUCTION PROCESS

    DEFF Research Database (Denmark)

    Jørgensen, Kirsten; Rasmussen, Grane Mikael Gregaard

    2009-01-01

    Function failures, defects and poor communication are major problems in the construction industry. These failures and defects are caused by a row of critical events in the construction process. The purpose of this paper is to define “critical events” in the construction process and to investigate...... cause-effects of failures and defects in the construction industry by using an analytical approach (The bowtie model) which is developed in the accident research. Using this model clarifies the relationships within the chain of failures that causes critical events with undesirable consequences....... In this way the causes of failures and the relationships between various failures are rendered visible. A large construction site was observed from start to finish as the empirical element in the research. The research focuses on all kinds of critical events identified throughout every phase during...

  16. Combinatorial designs constructions and analysis

    CERN Document Server

    Stinson, Douglas R

    2004-01-01

    Created to teach students many of the most important techniques used for constructing combinatorial designs, this is an ideal textbook for advanced undergraduate and graduate courses in combinatorial design theory. The text features clear explanations of basic designs, such as Steiner and Kirkman triple systems, mutual orthogonal Latin squares, finite projective and affine planes, and Steiner quadruple systems. In these settings, the student will master various construction techniques, both classic and modern, and will be well-prepared to construct a vast array of combinatorial designs. Design theory offers a progressive approach to the subject, with carefully ordered results. It begins with simple constructions that gradually increase in complexity. Each design has a construction that contains new ideas or that reinforces and builds upon similar ideas previously introduced. A new text/reference covering all apsects of modern combinatorial design theory. Graduates and professionals in computer science, applie...

  17. Statics of historic masonry constructions

    CERN Document Server

    Como, Mario

    2016-01-01

    This successful book, which is now appearing in its second edition, presents a comprehensive new Statics of Masonry Constructions. Masonry constructions are the great majority of the buildings in Europe’s historic centres and the most important monuments in its architectural heritage. Given the age of these constructions, the demand for safety assessments and restoration projects is pressing and constant. The book you hold in hands contributes to fill this demand. The second edition integrates the original text of the first edition with new developments, widening and revisions, due to recent research studies achievements. The result is a book that gives a complete picture of the behaviour of the Masonry Constructions. First of all, it gives the fundamentals of its Statics, based on the no-tension assumption, and then it develops the Limit Analysis for the Masonry Constructions. In this framework, through an interdisciplinary approach combining Engineering and Architecture, the book also investigates the sta...

  18. Collapse simulation of building constructions

    Directory of Open Access Journals (Sweden)

    Nekrest'yanov Viktor Nikolaevich

    Full Text Available The physical reasons for building structures destruction are both the forces arising at stress-strain state of construction elements and external influences arising at emergency situations, as well as their moments, impulses and periodic impulses with the frequencies close to of fluctuations frequencies of construction elements. We shall call the mathematical calculation models for the parameters-reasons of destructions the basic models. The basic models of destruction of building structures elements allow not only providing necessary level of reliability and survivability of the elements and the construction as a whole already at the stage of their design, but also giving the chance, at their corresponding completion, to provide rational decisions on the general need of recovery works and their volume depending on destruction level. Especially important for rational design decisions development, which ensure the demanded constructional safety of building structures, is library creation of the basic mathematical models of standard processes of bearing elements destructions for standard construction designs for the purpose of the further forecast (assessment of the level and probabilities of standard destructions. Some basic mathematical models of destructions processes of the standard elements of building structures are presented in the present article. A model of accounting for construction defects and a model of obtaining requirements to probabilities of partial destructions of a construction are given. Both of these models are probabilistic.

  19. Data Policy Construction Set - Building Blocks from Childhood Constructions

    Science.gov (United States)

    Fleischer, Dirk; Paul-Stueve, Thilo; Jobmann, Alexandra; Farrenkopf, Stefan

    2016-04-01

    A complete construction set of building blocks usually comes with instructions and these instruction include building stages. The products of these building stages usually build from very general parts become highly specialized building parts for very unique features of the whole construction model. This sounds very much like the construction or organization of an interdisciplinary research project, institution or association, doesn't it! The creation process of an overarching data policy for a project group or institution is exactly the combination of individual interests with the common goal of a collaborative data policy and can be compared with the building stages of a construction set of building blocks and the building instructions. Keeping this in mind we created the data policy construction set of textual building blocks. This construction set is subdivided into several building stages or parts each containing multiple building blocks as text blocks. By combining building blocks of all subdivisions it is supposed to create a cascading data policy document. Cascading from the top level as a construction set provider for all further down existing levels such as project, themes, work packages or Universities, faculties, institutes down to the working level of working groups. The working groups are picking from the remaining building blocks in the provided construction set the suitable blocks for its working procedures to create a very specific policy from the available construction set provided by the top level community. Nevertheless, if a working group realized that there are missing building blocks or worse that there are missing building parts, then they have the chance to add the missing pieces to the construction set of direct an future use. This cascading approach enables project or institution wide application of the encoded rules from the textual level on access to data storage infrastructure. This structured approach is flexible enough to allow for

  20. Hydrofluoric acid poisoning

    Science.gov (United States)

    Fluorhydric acid ... stomach, or intestine have holes (perforations) from the acid. ... Hydrofluoric acid is especially dangerous. The most common accidents involving hydrofluoric acid cause severe burns on the skin ...

  1. I. Monographic Section Constructing territories/constructing identity. Lagoons compared

    Directory of Open Access Journals (Sweden)

    Sauro Gelichi (a cura di

    2015-10-01

    Though fragmented and tied to the specificity of the single contributions, the picture that emerges is one moving towards the construction of a unitary interpretative paradigm, in which the constructed territory is analysed in all its forms and expressions, both material and ideological. The result is an original and in some ways unique story of a significant segment of early medieval Italian history that has until now been largely neglected by historical studies.

  2. Passive-solar construction handbook

    Energy Technology Data Exchange (ETDEWEB)

    Levy, E.; Evans, D.; Gardstein, C.

    1981-02-01

    Many of the basic elements of passive solar design are reviewed. Passive solar construction is covered according to system type, each system type discussion including a general discussion of the important design and construction issues which apply to the particular system and case studies illustrating designed and built examples of the system type. The three basic types of passive solar systems discussed are direct gain, thermal storage wall, and attached sunspace. Thermal performance and construction information is presented for typical materials used in passive solar collector components, storage components, and control components. Appended are an overview of analysis methods and a technique for estimating performance. (LEW)

  3. Landscape Construction in Dynamical Systems

    Science.gov (United States)

    Tang, Ying; Yuan, Ruoshi; Wang, Gaowei; Ao, Ping

    The idea of landscape has been recently applied to study various of biological problems. We demonstrate that a dynamical structure built into nonlinear dynamical systems allows us to construct such a global optimization landscape, which serves as the Lyapunov function for the ordinary differential equation. We find exact constructions on the landscape for a class of dynamical systems, including a van der Pol type oscillator, competitive Lotka-Volterra systems, and a chaotic system. The landscape constructed provides a new angle for understanding and modelling biological network dynamics.

  4. Concurrent Delay in Construction Disputes

    DEFF Research Database (Denmark)

    Cavaleri, Sylvie Cécile

    period of delay can potentially be attributed to several events falling within both parties' spheres of responsibility, commonly termed concurrent delay, is rarely regulated in construction contracts in spite of its common occurrence. This book analyses both the theoretical foundations and the practical......Delay is one of the issues most frequently encountered in today’s construction industry; it causes significant economic damage to all parties involved. Construction contracts, standard and bespoke, almost invariably consider delay from a perspective of single liability. If the event causing...

  5. Recombinant constructs of Borrelia burgdorferi

    Energy Technology Data Exchange (ETDEWEB)

    Dattwyler, Raymond J. (Setauket, NY); Gomes-Solecki, Maria J. C. (New York, NY); Luft, Benjamin J. (East Setauket, NY); Dunn, John J.(Bellport, NY)

    2007-02-20

    Novel chimeric nucleic acids, encoding chimeric Borrelia proteins comprising OspC or an antigenic fragment thereof and OspA or an antigenic fragment thereof, are disclosed. Chimeric proteins encoded by the nucleic acid sequences are also disclosed. The chimeric proteins are useful as vaccine immunogens against Lyme borreliosis, as well as for immunodiagnostic reagents.

  6. Isothermal Amplification of Nucleic Acids.

    Science.gov (United States)

    Zhao, Yongxi; Chen, Feng; Li, Qian; Wang, Lihua; Fan, Chunhai

    2015-11-25

    Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently accumulates nucleic acid sequences at constant temperature. Since the early 1990s, various isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR). These isothermal amplification methods have been used for biosensing targets such as DNA, RNA, cells, proteins, small molecules, and ions. The applications of these techniques for in situ or intracellular bioimaging and sequencing have been amply demonstrated. Amplicons produced by isothermal amplification methods have also been utilized to construct versatile nucleic acid nanomaterials for promising applications in biomedicine, bioimaging, and biosensing. The integration of isothermal amplification into microsystems or portable devices improves nucleic acid-based on-site assays and confers high sensitivity. Single-cell and single-molecule analyses have also been implemented based on integrated microfluidic systems. In this review, we provide a comprehensive overview of the isothermal amplification of nucleic acids encompassing work published in the past two decades. First, different isothermal amplification techniques are classified into three types based on reaction kinetics. Then, we summarize the applications of isothermal amplification in bioanalysis, diagnostics, nanotechnology, materials science, and device integration. Finally, several challenges and perspectives in the field are discussed.

  7. Workshop presentation: research guidelines for Construction Management

    OpenAIRE

    Marco Alvise Bragadin

    2013-01-01

    Nowadays the European economic system challenges the construction sector to take part to industrial recovery of western countries. In co-operation with the Construction Production research group of the Tampere University of of research about construction management tools and methods were detected. Research guidelines: 1) Construction management: tools and methods to manage construction projects 2) environmental impact of construction projects 3) construction management and safety 4) project p...

  8. Conceptual Model for Systematic Construction Waste Management

    OpenAIRE

    Abd Rahim Mohd Hilmi Izwan; Kasim Narimah

    2017-01-01

    Development of the construction industry generated construction waste which can contribute towards environmental issues. Weaknesses of compliance in construction waste management especially in construction site have also contributed to the big issues of waste generated in landfills and illegal dumping area. This gives sign that construction projects are needed a systematic construction waste management. To date, a comprehensive criteria of construction waste management, particularly for const...

  9. Sequential Construction of Costly Networks

    Energy Technology Data Exchange (ETDEWEB)

    Gutfraind, Alexander [Los Alamos National Laboratory

    2011-01-01

    Natural disasters or attacks often disrupt infrastructure networks requiring a costly recovery. This motivates an optimization problem where the objecitve is to construct the nodes of a graph G(V;E), and the cost of each node is dependent on the number of its neighbors previously constructed, or more generally, any properties of the previously-completed subgraph. In this optimization problem the objective is to find a permutation of the nodes which results in the least construction cost. We prove that in the case where the cost of nodes is a convex function in the number of neighbors, the optimal construction sequence is to start at a single node and move outwards. We also introduce algorithms and heuristics for solving various instances of the problem. Those methods can be applied to help reduce the cost of recovering from disasters as well as to plan the deployment of new network infrastructure.

  10. Fsusy and Field Theoretical Construction

    CERN Document Server

    Sedra, M B

    2009-01-01

    Following our previous work on fractional spin symmetries (FSS) \\cite{6, 7}, we consider here the construction of field theoretical models that are invariant under the $D=2(1/3,1/3)$ supersymmetric algebra.

  11. Construction Minerals Operations - Direct Download

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This map layer includes construction minerals operations in the United States. The data represent commodities covered by the Minerals Information Team (MIT) of the...

  12. Teen Brain: Still Under Construction

    Science.gov (United States)

    ... Brain Reprints For more information Share The Teen Brain: Still Under Construction Download PDF Download ePub Order ... the ups and downs of adolescence. The "Visible" Brain A clue to the degree of change taking ...

  13. What Should Guide Reality Construction?

    DEFF Research Database (Denmark)

    Ravn, Ib

    1991-01-01

    The constructivist movement in the social sciences (Berger & Luckmann, Gergen) argues that social institutions are the constructions of a community of human agents. This implies that people possess the power to radically change those institutions. This raises what may be called the values problem...... in constructivism, which, in its simplest form, is put in the title of the present chapter: What should guide reality construction? What principles or values should guide our construction of economic institutions, foreign policies, roles for the sexes, schooling, care for the sick? This values problem......-constructing the reality of her hosts. This acknowledgement implies responsibility and, hence, a personal set of values. If the values problem is central in constructivism as a general outlook on the world, it is even more so in the practice of constructivist research, since the values aspect has been actively suppressed...

  14. Dehydroabietic acid

    Directory of Open Access Journals (Sweden)

    Xiao-Ping Rao

    2009-10-01

    Full Text Available The title compound [systematic name: (1R,4aS,10aR-7-isopropyl-1,4a-dimethyl-1,2,3,4,4a,9,10,10a-octahydrophenanthrene-1-carboxylic acid], C20H28O2, has been isolated from disproportionated rosin which is obtained by isomerizing gum rosin with a Pd-C catalyst.. Two crystallographically independent molecules exist in the asymmetric unit. In each molecule, there are three six-membered rings, which adopt planar, half-chair and chair conformations. The two cyclohexane rings form a trans ring junction with the two methyl groups in axial positions. The crystal structure is stabilized by intermolecular O—H...O hydrogen bonds.

  15. Utilizing platforms in industrialized construction

    DEFF Research Database (Denmark)

    Bonev, Martin; Wörösch, Michael; Hvam, Lars

    2015-01-01

    construction companies. A promising approach adapted by operations management and design theory regards individual building projects as the adjustment and recombination of components and processes from a set of predefined platforms, while configuration systems assure feasible building solutions. Design...... methods map structural platform characteristics so as to balance commonality and distinctiveness. Originality/value – This paper proposes a general theory of platform-based development and execution in the industrialised construction sector, which goes beyond concurrent approaches of standardising...

  16. Constructing a modern city machine

    DEFF Research Database (Denmark)

    Lindegaard, Hanne; Jørgensen, Ulrik

    1998-01-01

    Based on the Copenhagen sewers debates and constructions the role of changing perceptions of water, hygiene and environment is discussed in relation to the modernisation of cities by machinating flows and infrastructures.......Based on the Copenhagen sewers debates and constructions the role of changing perceptions of water, hygiene and environment is discussed in relation to the modernisation of cities by machinating flows and infrastructures....

  17. On Lawson-Osserman Constructions

    OpenAIRE

    Xu, Xiaowei; Yang, Ling; Zhang, Yongsheng

    2016-01-01

    Lawson-Osserman constructed three types of non-parametric minimal cones of higher codimension based on Hopf fibrations between Euclidean spheres, which can been seen as Lipschitz solutions to the minimal surface equations which are not differentiable, thereby making sharp contrast to the regularity theorem for minimal hypersurfaces in Euclidean spaces. In this paper, the above constructions are generalized in a more general scheme. Once a mapping f can be written as the composition of a Riema...

  18. User Driven Innovation Construct Development

    Directory of Open Access Journals (Sweden)

    Tine Nagy

    2011-12-01

    Full Text Available In this paper by the literature review we interpreted changes in societyand other changing conditions that have contributed to the emergenceof user-driven innovation and a ‘design thinking’ approach. Thetwo areas are very similar in practice, so we suggest combining theminto a construct. The construct is a pioneer contribution to the developmentof science in the field of user-driven innovation and the designthinking approach.

  19. Design Processes and Constructive Ergonomics

    DEFF Research Database (Denmark)

    Broberg, Ole

    2015-01-01

    This paper discusses some central issues in the concept of constructive ergonomics. It does so by reflecting on experiences from ergonomics intervention projects carried out in Denmark. Constructive ergonomics has a huge potential as a new way to go for ergonomics research and practice. However......, many challenges are to be overcome. They relate among others to education and training of ergonomist, and the cultural and institutional setting of ergonomics in specific countries....

  20. RISK MANAGEMENT IN CONSTRUCTION INDUSTRY

    OpenAIRE

    V. Sankara Subramaniyan; K. Veerakumar

    2017-01-01

    Construction industry is highly risk prone, with complex and dynamic project environments which create an atmosphere of high uncertainty and risk. The industry is vulnerable to various technical, socio-political and business risks. The track record to cope with these risks has not been very good in construction industry. Risk management is a concept which becomes very popular in a number of businesses. Many companies often establish a risk management procedure in their projects for improving ...

  1. Design Processes and Constructive Ergonomics

    OpenAIRE

    Broberg, Ole

    2015-01-01

    This paper discusses some central issues in the concept of constructive ergonomics. It does so by reflecting on experiences from ergonomics intervention projects carried out in Denmark. Constructive ergonomics has a huge potential as a new way to go for ergonomics research and practice. However, many challenges are to be overcome. They relate among others to education and training of ergonomist, and the cultural and institutional setting of ergonomics in specific countries.

  2. Green Construction in Building Renovation

    Directory of Open Access Journals (Sweden)

    Ksit Barbara

    2016-06-01

    Full Text Available Modern materials and construction solutions draw more and more attention to ecology and building certification. Among the criteria appearing in revitalization, an important element is bringing plants back into heavily urbanized areas. In its natural form, this is not possible to carry out everywhere, often requiring large amounts of space. Nowadays, however, there are a number of green roofs and green wall systems, allowing “greener” construction without making significant changes in the urban environment.

  3. Green Construction in Building Renovation

    OpenAIRE

    Ksit Barbara; Majcherek Michał

    2016-01-01

    Modern materials and construction solutions draw more and more attention to ecology and building certification. Among the criteria appearing in revitalization, an important element is bringing plants back into heavily urbanized areas. In its natural form, this is not possible to carry out everywhere, often requiring large amounts of space. Nowadays, however, there are a number of green roofs and green wall systems, allowing “greener” construction without making significant changes in the urba...

  4. ConStruct: Improved construction of RNA consensus structures

    Directory of Open Access Journals (Sweden)

    Steger Gerhard

    2008-04-01

    Full Text Available Abstract Background Aligning homologous non-coding RNAs (ncRNAs correctly in terms of sequence and structure is an unresolved problem, due to both mathematical complexity and imperfect scoring functions. High quality alignments, however, are a prerequisite for most consensus structure prediction approaches, homology searches, and tools for phylogeny inference. Automatically created ncRNA alignments often need manual corrections, yet this manual refinement is tedious and error-prone. Results We present an extended version of CONSTRUCT, a semi-automatic, graphical tool suitable for creating RNA alignments correct in terms of both consensus sequence and consensus structure. To this purpose CONSTRUCT combines sequence alignment, thermodynamic data and various measures of covariation. One important feature is that the user is guided during the alignment correction step by a consensus dotplot, which displays all thermodynamically optimal base pairs and the corresponding covariation. Once the initial alignment is corrected, optimal and suboptimal secondary structures as well as tertiary interaction can be predicted. We demonstrate CONSTRUCT's ability to guide the user in correcting an initial alignment, and show an example for optimal secondary consensus structure prediction on very hard to align SECIS elements. Moreover we use CONSTRUCT to predict tertiary interactions from sequences of the internal ribosome entry site of CrP-like viruses. In addition we show that alignments specifically designed for benchmarking can be easily be optimized using CONSTRUCT, although they share very little sequence identity. Conclusion CONSTRUCT's graphical interface allows for an easy alignment correction based on and guided by predicted and known structural constraints. It combines several algorithms for prediction of secondary consensus structure and even tertiary interactions. The CONSTRUCT package can be downloaded from the URL listed in the Availability and

  5. 脊髓源星形胶质细胞胶质原纤维酸性蛋白表达抑制最佳短发夹样RNA分子筛选及真核表达载体构建%Optimal screening of short hairpin and construction of its eukaryotic expression vector for glial fibrillary acidic protein

    Institute of Scientific and Technical Information of China (English)

    高明勇; 李振宇; 闫洪印

    2007-01-01

    BACKGROUND: The glial scar formation after spinal cord injury in mammals is the physical and chemical barriers for neural regeneration, and relieving or delaying glial scar formation can provide benefit conditions for the regeneration of injured spinal cord.OBJECTIVE: To design and screen short hairpin RNA (shRNA) interfere molecular targeting the gene coding region of glial fibrillary acidic protein (GFAP) in rat, and reconstruct the eukaryotic vector of shRNA.DESIGN: An observational animal experiment.SETTING: Department of Spine Surgery, Shenzhen Hospital affiliated to Southern Medical University.MATERIALS: Twenty-five Wistar rats of clean degree, either male or female, weighing 20-25 g, were used. DMEM/F12,lipofectamine2000, Trizol RNA separating kits); fetal bovine serum (Hyclone); BamH Ⅰ, HindⅢ, Pstl, Sail and T4 ligases;Plasmid mini preparation kit and DNA gel extraction kit.METHODS: The experiments were carried out in the Shenzhen Hospital affiliated to Southern Medical University from October 2005 to June 2006. Three pairs of shRNA template which composed of 19 bp reverse repeated motif of GFAP target sequence with 9 bp spacer were designed and synthesized, then they were inserted directionally into plasmid Psilencer 2.1 respectively to generate small interfering RNA (siRNA) eukaryotic expression vector. ShRNA molecules were transfected by liposome via ex vivo expression repressive model of GFAP of rat spinal astrocytes. The effects of expressive suppression were detected with real-time fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, and then the optimal shRNA eukaryotic vector of repressive expression of GFAP was screened.MAIN OUTCOME MEASURES: ① Interfering sequence specific shRNA template synthesis; ② Constructing specific recombinant plasmid eukaryotic expression vector. ③ Culturing rat spinal astrocytes in vitro; ④ Effects of expressive suppression on GFAP in primary astrocytes after RNA

  6. Investigation of Groundwater Flow at Highway Construction Areas in Korea

    Science.gov (United States)

    Choi, Y.; Park, Y.; Ji, S.; Cheong, Y.; Yim, G.

    2006-05-01

    Contamination by acid rock drainage was found at highway construction areas in Korea, where pyrites were included in materials to raise the ground level. To remediate the acid rock drainage, groundwater flow direction and total flow rate were investigated in addition to the relationship between groundwater and surface water. Multiple boreholes were installed for geological structure surveys, pumping tests, slug test and tracer tests. Geological survey showed that a water-table aquifer system included a relatively homogeneous earthen layer and an underlying undisturbed alluvial layer. Transmissivity and storativity of the upper layer were investigated 0.1-2.6m2/day and 0.3 relatively by pumping tests. Hydraulic conductivity of the upper layer was investigated 0.1m/day by slug tests. Chloride ion was used in tracer tests, which included a natural gradient method and a push-pull method. In the natural gradient method, it was failed to detect chloride ion in groundwater. In the push-pull test, dispersivity ranges from 0.001m to 0.3m for several drift time. With the characteristic parameters from aquifer tests and tracer tests, numerical modeling techniques were used to evaluate groundwater flow directions and rates. Boundary conditions were decided to reflect geological and geographical boundaries, like concrete barriers, water divides and rivers. Numerical simulations showed the differences between groundwater flow before constructions and that after constructions. After the highway constructions are finished, groundwater direction changes seriously and total amount of the acid rock drainage is estimated 166.5m3/day. To find out the effect of precipitation changes, several numerical simulations were performed. It was shown that total amount of the acid rock drainage ranges from 73.8m3/day in the dry season to 323.6m3/day in the rainy season.

  7. Feasibility of constructing artificial cartilage with rabbit mesenchymal stem cells and polyglycolic acid scaffold%兔骨髓间充质干细胞及聚羟基乙酸支架材料构建人工软骨的可行性

    Institute of Scientific and Technical Information of China (English)

    杨大鉴; 黄定强; 胥方元

    2007-01-01

    ,但质地明显较硬.而第6,12周的对照组的标本大部分均被吸收,以第12周的标本更明显,且均未见软骨样组织形成.②支架植入实验兔体内后6及12周不同染色观察结果及Ⅱ型胶原蛋白的表达:苏木精-伊红染色结果显示:6周时,有软骨陷窝样结构开始形成,聚羟基乙酸支架开始降解;12周时,复合物呈现软骨组织样的形态,有较明显的软骨陷窝样结构形成,细胞排列规则,成群存在于软骨陷窝样结构内,边缘的细胞小,中央的细胞大,聚羟基乙酸基本完全消失.阿新蓝染色结果:6周时,组织内有部分区域被染成淡蓝色;12周时组织的基质被广泛染成蓝紫色,说明有大量的酸性粘多糖形成.苯胺蓝染色结果:6周时,组织内可见蓝色异染性基质;12周时组织内呈现很强的蓝色异染.免疫组织化学染色结果:6周时,胞浆内可见棕黄色阳性颗粒,基质内有少量的Ⅱ型胶原蛋白表达;12周时,胞浆、基质内均有较强的阳性表达.Ⅱ型胶原蛋白的表达:6周时,胞浆内可见深棕黄色阳性颗粒,基质内有少量的Ⅱ型胶原mRNA表达;12周时,胞浆、基质内均有强的阳性表达.对照组标本基本上已被吸收,没有成形的组织工程化标本.结论:兔骨髓间充质干细胞在诱导剂作用下,经体外和体内培养后,可生成组织工程化类软骨.%BACKGROUND: Whether choosing a suitable biological scaffold compounding with mesenchymal stem cells (MSCs) can construct an ideal tissue-engineering cartilage or not should be researched further.OBJ ECTIVE: To investigate the feasibility of constructing artificial cartilage by using amplified rabbit MSCs which were inoculated on poly-glycolic acid (PGA).DESIGN: Single sample observation.SETTING: Department of Otolaryngology-Head & Neck Surgery, Affiliated Hospital of Sichuan Luzhou Medical College.MATERIALS: The experiment was carried out in the Luzhou Medical College from October 2004 to October 2005. A total of 8 Japanese large

  8. Review on Chlorobenzoic Acids Biodegradation and Their Environmental Impacts

    Institute of Scientific and Technical Information of China (English)

    LuWenming; QiYun; ZhaoLin; TanXin

    2005-01-01

    Chlorobenzoic Acids are toxic organic compounds largely distributed in soils and sediments. They can be degraded to various products by microorgans. This paper is a review of the literature on biodegradability of the chlorobenzoic acids. The degradation pathways, degradation genes, role of transposable elements, and construction of strains are discussed. A brief introduction is given on the environmental impacts and the pollution control.

  9. Human Factors Simulation in Construction Management Education

    Science.gov (United States)

    Jaeger, M.; Adair, D.

    2010-01-01

    Successful construction management depends primarily on the representatives of the involved construction project parties. In addition to effective application of construction management tools and concepts, human factors impact significantly on the processes of any construction management endeavour. How can human factors in construction management…

  10. 50 CFR 29.21-5 - Construction.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Construction. 29.21-5 Section 29.21-5... Construction. (a) If construction is not commenced within two (2) years after date of right-of-way grant, the...) Proof of construction: Upon completion of construction, the applicant shall file a certification...

  11. 46 CFR 160.054-3 - Construction.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Construction. 160.054-3 Section 160.054-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS... Construction. (a) Construction. The container shall be constructed of tough transparent material, not less...

  12. 23 CFR 660.113 - Construction.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Construction. 660.113 Section 660.113 Highways FEDERAL... (DIRECT FEDERAL) Forest Highways § 660.113 Construction. (a) No construction shall be undertaken on any FH... construction of FHs will be performed by the contract method, unless construction by the FHWA, the FS, or...

  13. Construction Cost Analysis : Residential Construction Demonstration Project Cycle II.

    Energy Technology Data Exchange (ETDEWEB)

    Barnett, Cole; Thor, Philip W.

    1990-06-01

    The Residential Construction Demonstration Project (RCDP) is designed to demonstrate new residential building techniques and product innovations which advance the stage-of-the-art in constructing energy-efficient electrically heated residences. A secondary purpose is to obtain documented cost and energy savings data from which to make accurate assessments of the cost-effectiveness of various conservation innovations. The project solicits participation of regional homebuilders by offering them financial incentives for constructing homes to the Model Conservation Standards (MCS) and including at least one innovation.'' The innovations are determined by BPA and the States prior to construction and represent construction techniques or energy saving products that might reduce the cost of building MCS homes, or expand the options available to builders in achieving MCS levels of energy efficiency in homes. Besides covering some of the additional risk for employing the innovation, the incentive payment guarantees that builders will provide certain amounts of information regarding the cost and acceptability of building the homes. In addition, an incentive is paid to homeowners for their participation in data collection efforts following construction. Several one-time'' tests were performed on the houses and homeowners were required to report energy consumption and temperature data on a weekly basis for approximately 18 months. BPA and the States compile the information obtained from the builders and homeowners. Access to this data is provided for the purpose of analyzing the cost and performance of the RCDP homes, as well as understanding the value of the various innovations that are tested. 25 tabs., 4 figs.

  14. L-缬氨酸产生菌JVHK597的选育及无机盐对其产酸量的影响%Breeding of of L-valine Producing Strain JVHK597 and Influence of Inorganic Salt on Its Acid Production

    Institute of Scientific and Technical Information of China (English)

    刘焕民; 葛向阳; 张伟国

    2011-01-01

    [目的]筛选L-缬氨酸高产菌株并研究其发酵条件.[方法]以黄色短杆菌(Brebvibacterium flavum)突变株ZGH61.28为出发菌株,采用紫外线(UV)、硫酸二乙酯(DES)和亚硝基胍(NTG)3种诱变剂进行诱变处理,通过摇瓶培养筛选出L-缬氨酸高产突变菌株.[结果]经过UV、DES和NTG3种诱变剂处理菌株ZGH6128,逐步获得菌株JVHK597,并具有Leu营养缺陷、Ile营养缺陷、Met营养缺陷、α-AB抗性、2-TA抗性5种遗传标记.在未优化的条件下,菌株JVHK597摇瓶发酵72 h的产酸量达41.2 g/L.8次传代试验结果表明,菌株JVHK597的产酸能力稳定,经鉴定,菌株JVHK597的基因型为(Leu-,Ile-,Met-,α-ABr,2 -TAr),遗传标记具有稳定性.发酵培养基中硫酸镁( MgSO4·7H2O)和磷酸二氢钾的含量分别为0.6和1.4 g/L时,最有利于菌株生产L-缬氨酸.[结论]试验筛选出了L-缬氨酸高产菌株JVHK597,并为其发酵培养提供了指导.%[Objective]The aim was to screen superior strain of L-valine and study its fermentation condition. [ Method] With the mutant strain ZGH6128 of Brebvibacterium flavum as initial strain, 3 kinds of mutagens of ultraviolet (UV), diethyl sulfate (DES) and nitrosoguani-dine (NTG) were used in its mutagene treatments, its superior mutant strain of L-valine was screened out through shaking culture. [ Result] The strain ZGH6128 was treated with 3 kinds of mutagens of UV, DES and NTG and the mutant strain JVHK597 was obtained progressively. This mutant strain possessed 5 genetic marks of Leu auxotroph, Ile auxotroph, Met auxotroph, a-AB resistance and 2-TA resistance. Under non-optimized condition, the production of acid from the strain JVHK597 reached 41.2 g/L after fermentation for 72 h. The experimental results of 8 passages showed that the acid producing ability of the strain JVHK597 was stable and its genotype was identified to be (Leu-, Ile-, Met-, α-AB', 2-TA') and its genetic marks had stability. When the contents of bitter salt (Mg

  15. Health Profile of Construction Workers in Hong Kong

    Directory of Open Access Journals (Sweden)

    Wen Yi

    2016-12-01

    Full Text Available Construction is a manual, heavy, and complex sector concerning the most fatal accidents and high incidence of occupational illnesses and injuries resulting in days away from work. In Hong Kong, “Pilot Medical Examination Scheme for Construction Workers” was launched in 2014 to detect the health problems of their construction workforce. All registered workers under the Construction Workers Registration Board are eligible to join the scheme. The purpose of this paper is to assess the physical condition, physiological status, and musculoskeletal disorders of 942 construction workers in Hong Kong. This study adopted a two-phase design, which includes a basic medical examination to measure the workers’ physiological parameters, such as blood pressure, resting heart rate, glucose, cholesterol, uric acid, liver function test, and renal function test; as well as a face-to-face interview following the medical examination to collect their demographic information and pain experience. Individual characteristics, including gender, age, obesity, alcohol drinking habit, and sleeping habit influenced the health condition of construction workers. Among the participants, 36.1% and 6.5% of them were overweight and obese, respectively. In addition, 43.0%, 38.4%, 16.2%, and 13.9% of the participants exceeded the thresholds of cholesterol, blood pressure, urea nitrogen, and uric urea, correspondingly. Moreover, 41.0% of the participants suffered musculoskeletal pain, where the most frequent painful parts occur in the lower back, shoulder, knees, leg, and neck. Through these findings, a series of important issues that need to be addressed is pointed out in terms of maintaining the physical well-being and reducing musculoskeletal disorders of construction workers. The finding may have implications for formulating proper intervention strategies for the sustainable development of Hong Kong’s construction industry.

  16. Creatinine related reference ranges for urinary homovanillic acid and vanillylmandelic acid at 6 months of age.

    OpenAIRE

    M Cole; Parker, L.; Craft, A W; Bell, S.; G. Dale; McGill, A C; Seviour, J. A.; Smith, J

    1993-01-01

    The relationship between homovanillic acid (HVA), vanillylmandelic acid (VMA), and creatinine in the urine of 6 month old babies has been studied and reference ranges in the form of centiles constructed for HVA and VMA against creatinine. Over 10,000 urine samples were collected from babies in four health districts in the north of England. HVA and VMA concentration, either independently or when divided by creatinine concentration, were dependent upon the absolute concentration of creatinine i...

  17. Constructing entanglement measures for fermions

    Science.gov (United States)

    Johansson, Markus; Raissi, Zahra

    2016-10-01

    In this paper we describe a method for finding polynomial invariants under stochastic local operations and classical communication (SLOCC) for a system of delocalized fermions shared between different parties, with global particle-number conservation as the only constraint. These invariants can be used to construct entanglement measures for different types of entanglement in such a system. It is shown that the invariants, and the measures constructed from them, take a nonzero value only if the state of the system allows for the observation of Bell-nonlocal correlations. Invariants of this kind are constructed for systems of two and three spin-1/2 fermions and examples of maximally entangled states are given that illustrate the different types of entanglement distinguished by the invariants. A general condition for the existence of SLOCC invariants and their associated measures is given as a relation between the number of fermions, their spin, and the number of spatial modes of the system. In addition, the effect of further constraints on the system, including the localization of a subset of the fermions, is discussed. Finally, a hybrid Ising-Hubbard Hamiltonian is constructed for which the ground state of a three-site chain exhibits a high degree of entanglement at the transition between a regime dominated by on-site interaction and a regime dominated by Ising interaction. This entanglement is well described by a measure constructed by the introduced method.

  18. Modification Management for Construction Processes

    Institute of Scientific and Technical Information of China (English)

    Wolfgang Huhnt; Sven Richter; Felix Enge

    2008-01-01

    This paper presents an approach to handle different types of subsequent modifications in con-struction processes. It is focused on a formalized approach with the objective that effects of subsequent modifications are detected automatically. The approach presented in this paper makes use of an existing modeling technique specifically focused on construction processes. The modeling techniques result in a process description where all technological interdependencies between construction activities are consid-ered and where consistency between the sequence of activities and the history of components is guaran-teed. This modeling technique is expanded by an existing concept for handling versions and dependencies between objects. The integration of these different concepts makes it possible to track effects of relevant types of subsequent modifications in construction processes on a formalized basis. Effects of subsequent modifications are detected by algorithms, different versions can be compared by algorithms and different versions are stored so that subsequent modifications are documented. The innovation of the approach pre-sented is the completeness. Effects of different relevant types of subsequent modifications in construction processes are tracked on a formalized basis.

  19. Soybean phytase and nucleic acid encoding the same

    OpenAIRE

    1999-01-01

    Isolated soybean phytase polypeptides and isolated nucleic acids encoding soybean phytases are provided. The invention is also directed to nucleic acid expression constructs, vectors, and host cells comprising the isolated soybean phytase nucleic acids, as well as methods for producing recombinant and non-recombinant purified soybean phytase. The invention also relates to transgenic plants expressing the soybean phytase, particularly expression under seed-specific expression control elements.

  20. Derived bracket construction up to homotopy

    CERN Document Server

    Uchino, K

    2012-01-01

    We will introduce the notion of higher derived bracket construction in the category of operads and prove that the higher derived bracket construction of Lie operad is equivalent with the cobar construction of Leibniz operad.

  1. Construct ability Improvement for Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dae Soo; Lee, Jong Rim; Kim, Jong Ku [Korea Electric Power Research Institute, Taejon (Korea, Republic of)

    1997-12-31

    The purpose of this study was to identify methods for improving the construct ability of nuclear power plants. This study reviewed several references of current construction practices of domestic and overseas nuclear plants in order to identify potential methods for improving construct ability. The identified methods for improving construct ability were then evaluated based on the applicability to domestic nuclear plant construction. The selected methods are expected to reduce the construction period, improve the quality of construction, cost, safety, and productivity. Selection of which methods should be implemented will require further evaluation of construction modifications, design changes, contract revisions. Among construction methods studied, platform construction methods can be applied through construction sequence modification without significant design changes, and Over the Top construction method of the NSSS, automatic welding of RCL pipes, CLP modularization, etc., are considered to be applied after design modification and adjustment of material lead time. (author). 49 refs., figs., tabs.

  2. The role for an invariant aspartic acid in hypoxanthine phosphoribosyltransferases is examined using saturation mutagenesis, functional analysis, and X-ray crystallography.

    Science.gov (United States)

    Canyuk, B; Focia, P J; Eakin, A E

    2001-03-01

    The role of an invariant aspartic acid (Asp137) in hypoxanthine phosphoribosyltransferases (HPRTs) was examined by site-directed and saturation mutagenesis, functional analysis, and X-ray crystallography using the HPRT from Trypanosoma cruzi. Alanine substitution (D137A) resulted in a 30-fold decrease of k(cat), suggesting that Asp137 participates in catalysis. Saturation mutagenesis was used to generate a library of mutant HPRTs with random substitutions at position 137, and active enzymes were identified by complementation of a bacterial purine auxotroph. Functional analyses of the mutants, including determination of steady-state kinetic parameters and pH-rate dependence, indicate that glutamic acid or glutamine can replace the wild-type aspartate. However, the catalytic efficiency and pH-rate profile for the structural isosteric mutant, D137N, were similar to the D137A mutant. Crystal structures of four of the mutant enzymes were determined in ternary complex with substrate ligands. Structures of the D137E and D137Q mutants reveal potential hydrogen bonds, utilizing several bound water molecules in addition to protein atoms, that position these side chains within hydrogen bond distance of the bound purine analogue, similar in position to the aspartate in the wild-type structure. The crystal structure of the D137N mutant demonstrates that the Asn137 side chain does not form interactions with the purine substrate but instead forms novel interactions that cause the side chain to adopt a nonfunctional rotamer. The results from these structural and functional analyses demonstrate that HPRTs do not require a general base at position 137 for catalysis. Instead, hydrogen bonding sufficiently stabilizes the developing partial positive charge at the N7-atom of the purine substrate in the transition-state to promote catalysis.

  3. Algorithms for Decision Tree Construction

    KAUST Repository

    Chikalov, Igor

    2011-01-01

    The study of algorithms for decision tree construction was initiated in 1960s. The first algorithms are based on the separation heuristic [13, 31] that at each step tries dividing the set of objects as evenly as possible. Later Garey and Graham [28] showed that such algorithm may construct decision trees whose average depth is arbitrarily far from the minimum. Hyafil and Rivest in [35] proved NP-hardness of DT problem that is constructing a tree with the minimum average depth for a diagnostic problem over 2-valued information system and uniform probability distribution. Cox et al. in [22] showed that for a two-class problem over information system, even finding the root node attribute for an optimal tree is an NP-hard problem. © Springer-Verlag Berlin Heidelberg 2011.

  4. TALENs Construction: Slowly but Surely.

    Science.gov (United States)

    Hegazy, Wael Abdel Halim; Youns, Mahmoud

    2016-01-01

    Cancer is thought to be a direct result of transcriptional misregulation. Broad analysis of transcriptional regulatory elements in healthy and cancer cells is needed to understand cancer development. Nucleases regulatory domains are recruited to bind and manipulate a specific genomic locus with high efficacy and specificity. TALENs (transcription activatorlike effector nuclease) fused to endonuclease FokI have been used widely to target specific sequences to edit several genes in healthy and cancer cells. This approach is promising to target specific cancer genes and for this purpose it is needed to pack such TALENs into viral vectors. There are some considerations which control the success of this approach, targeting appropriate sequences with efficient construction of TALENs being crucial factors. We face some obstacles in construction of TALENs; in this study we made a modification to the method of Cermk et al 2011 and added one step to make it easier and increase the availability of constructs.

  5. Construction and compression of Dwarf

    Institute of Scientific and Technical Information of China (English)

    XIANG Long-gang; FENG Yu-cai; GUI Hao

    2005-01-01

    There exists an inherent difficulty in the original algorithm for the construction of Dwarf, which prevents it from constructing true Dwarfs. We explained when and why it introduces suffix redundancies into the Dwarf structure. To solve this problem, we proposed a completely new algorithm called PID. It bottom-up computes partitions of a fact table, and inserts them into the Dwarf structure. Ifa partition is an MSV partition, coalesce its sub-Dwarf; otherwise create necessary nodes and cells. Our performance study showed that PID is efficient. For further condensing of Dwarf, we proposed Condensed Dwarf, a more compressed structure, combining the strength of Dwarf and Condensed Cube. By eliminating unnecessary stores of "ALL" cells from the Dwarf structure, Condensed Dwarf could effectively reduce the size of Dwarf, especially for Dwarfs of the real world, which was illustrated by our experiments. Its query processing is still simple and, only two minor modifications to PID are required for the construction of Condensed Dwarf.

  6. Statics of Historic Masonry Constructions

    CERN Document Server

    Como, Mario

    2013-01-01

    Masonry constructions are the great majority of the buildings in Europe’s historic centres and the most important monuments in its architectural heritage. Given the age of much of these constructions, the demand for safety assessments and restoration projects is pressing and constant. This book aims to help fill this demand presenting a comprehensive new statics of masonry constructions. The book, result of thirty years of research and professional experience, gives the fundamentals of statics of the masonry solid, then applied to the study of statics of arches, piers and vaults. Further, combining engineering and architecture and through an interdisciplinary approach, the book investigates the statical behaviour of many historic monuments, as the Pantheon, the Colosseum,  the domes of S. Maria del Fiore in Florence and of St. Peter in Rome, the Tower of Pisa, the Gothic Cathedrals and the Masonry Buildings under seismic actions.

  7. Oxalic acid production by citric acid-producing Aspergillus niger overexpressing the oxaloacetate hydrolase gene oahA.

    Science.gov (United States)

    Kobayashi, Keiichi; Hattori, Takasumi; Honda, Yuki; Kirimura, Kohtaro

    2014-05-01

    The filamentous fungus Aspergillus niger is used worldwide in the industrial production of citric acid. However, under specific cultivation conditions, citric acid-producing strains of A. niger accumulate oxalic acid as a by-product. Oxalic acid is used as a chelator, detergent, or tanning agent. Here, we sought to develop oxalic acid hyperproducers using A. niger as a host. To generate oxalic acid hyperproducers by metabolic engineering, transformants overexpressing the oahA gene, encoding oxaloacetate hydrolase (OAH; EC 3.7.1.1), were constructed in citric acid-producing A. niger WU-2223L as a host. The oxalic acid production capacity of this strain was examined by cultivation of EOAH-1 under conditions appropriate for oxalic acid production with 30 g/l glucose as a carbon source. Under all the cultivation conditions tested, the amount of oxalic acid produced by EOAH-1, a representative oahA-overexpressing transformant, exceeded that produced by A. niger WU-2223L. A. niger WU-2223L and EOAH-1 produced 15.6 and 28.9 g/l oxalic acid, respectively, during the 12-day cultivation period. The yield of oxalic acid for EOAH-1 was 64.2 % of the maximum theoretical yield. Our method for oxalic acid production gave the highest yield of any study reported to date. Therefore, we succeeded in generating oxalic acid hyperproducers by overexpressing a single gene, i.e., oahA, in citric acid-producing A. niger as a host.

  8. PRIORITISING LEAN CONSTRUCTION BARRIERS IN UGANDA'S CONSTRUCTION INDUSTRY

    Directory of Open Access Journals (Sweden)

    Henry Mwanaki Alinaitwe

    2009-06-01

    Full Text Available Engaging in lean construction efforts could prove to be highly rewarding for building firms in Uganda. However, lean construction is risky and can be disastrous if not properly managed. Lean production efforts in some other countries have not been successful due to the many barriers to its successful implementation. To enable sound lean construction efforts and to increase the chances of success in eliminating waste, a thorough investigation of the barriers is essential. This study presents 31 barriers and investigates their influence (strength on the success of lean construction initiatives. Structured interviews were carried out with technical managers of building firms to assess their perception of the barriers to lean production based on their experience at their firms. The strongest barrier is the provision of inputs exactly when required. Additionally, the barriers were ranked according to the ease of overcoming each. The easiest barrier to overcome is keeping the required items in the right place. Finally, a graphical aid is provided to enable decision makers to concentrate their efforts on the influential (strong, yet easy to overcome barriers. A lack of buildable designs and a participative management style for the workforce are the most important barriers to successful waste reduction in terms of strength and ease of overcoming. On the other hand, a lack of an organisational culture that supports teamwork, a lack of prefabrication and a lack of knowledgeable and skilled workers are regarded as low in strength, and at the same time difficult to overcome.

  9. A Construction Management Framework for Mass Customisation in Traditional Construction

    Directory of Open Access Journals (Sweden)

    María Dolores Andújar-Montoya

    2015-04-01

    Full Text Available A Mass Customisation model is discussed as a competitive positioning strategy in the marketplace adding value to the customer’s end-use. It includes the user as part of the construction process responding to the customer’s demands and wishes. To the present day, almost all proposals for Mass Customisation have been focused on the design phase and single family houses. The reality is that the processes carried out in the work execution are so inefficient that the costs of the Mass Customisation models are assumed by the customer and they do not offer solutions that support the change management. Furthermore, this inefficiency often makes Mass Customisation unfeasible in terms of deadlines and site management. Therefore, the present proposal focuses on achieving the paradigm of Mass Customisation in the traditional residential construction complementary to the existing proposals in the design phase. All this through the proposal of a framework for the integral management in the work execution, which will address change management introduced by the users offering an efficient and productive model that reduces costs in the process. This model will focus on the synergy between different strategies, techniques and technologies currently used in the construction management (such as Lean Construction or Six Sigma, together with, other strategies and technologies that have proven to be valid solutions in other fields (such as Business Process Management, Service Oriented Architecture, etc..

  10. Specifications for Supplementary Classroom Units "Standard Construction" and "Preframed Construction".

    Science.gov (United States)

    Waring, Robert B.; And Others

    The standards that should be applied to the construction of supplementary classroom units are discussed in this report. Areas dealt with are--(1) general mechanical and electrical work, (2) concrete, (3) masonry, (4) miscellaneous steel and iron, (5) metal windows, (6) carpentry at site, (7) millwork, (8) acoustic treatment, (9) thermal…

  11. Constructing the Virtual Production Line

    Institute of Scientific and Technical Information of China (English)

    孙连胜; 宁汝新

    2003-01-01

    In order to study the constructing technology of virtual production line, the structure model of virtual production line was presented, and the object-oriented technique was used to establish its basic classes and relevant models, including solid model, behavior model and object-oriented Petri net based control model, and based on this, the constructing of virtual production line was realized. The application proved that the virtual production line had many good characteristics, such as visualization, interaction, multi-layer and reusability, and it's an efficient tool of analyzing and modeling for layout planning and rapidly reconfiguring of production line.

  12. Minimal surfaces for architectural constructions

    Directory of Open Access Journals (Sweden)

    Velimirović Ljubica S.

    2008-01-01

    Full Text Available Minimal surfaces are the surfaces of the smallest area spanned by a given boundary. The equivalent is the definition that it is the surface of vanishing mean curvature. Minimal surface theory is rapidly developed at recent time. Many new examples are constructed and old altered. Minimal area property makes this surface suitable for application in architecture. The main reasons for application are: weight and amount of material are reduced on minimum. Famous architects like Otto Frei created this new trend in architecture. In recent years it becomes possible to enlarge the family of minimal surfaces by constructing new surfaces.

  13. Construction of UHVAC Test Base

    Institute of Scientific and Technical Information of China (English)

    Yang Yingjian; Hu Yi; Wu Xiong; Wu Zhirong; Wan Qifa; Liu Xianjin; Cai Wei

    2009-01-01

    @@ The construction of the UHVAC test base of SGCC is an important component of the pilot project on UHVAC power transmission and transformation in China.This paper introduces the layout,technical requirements and main test functions of the UHVAC test base as well as difficulties during construction.It lays stress on the characterization of a 1 000-kV single-circuit test line,a 1 000-kV one-tower double-circuit test line,a full voltage test site for UHV equipment,a climate laboratory,a corona cage and a long-wave-front impulse voltage generator.

  14. Partnering in the Construction Industry

    DEFF Research Database (Denmark)

    Ditlev Bohnstedt, Kristian; Haugbølle, Kim; Bejder, Erik

    2013-01-01

    gaps in existing body of knowledge. The objective of this literature-based paper is to explore the current literature by systematically reviewing and summarizing research trends in leading top-tier construction management journals between 2002 and 2012, addressing how the themes in partnering related...... publications change across this period. It is concluded that trends such as research on developing trust among construction partners to facilitate project success, feasibility of and the barriers to adopting a partnering approach and strategic propositions for overcoming barriers are highly emerging. Finally...

  15. Constructing Pairs of Compatible Characters

    Institute of Scientific and Technical Information of China (English)

    Mathias Kratzer

    2003-01-01

    Given a finite group X such that both the conjugacy of elements in X and the length of any conjugacy class in X can be decided/computed efficiently,the first algorithm described in this article constructs a uniquely determined sequence of representatives for all the conjugacy classes of X. In particular, based on this sequence, any two characters of different groups isomorphic to X become comparable against each other which is utilized by a second algorithm designed to construct so-called compatible characters of given finite groups G and H having isomorphic subgroups U ≤ G and V ≤ H, respectively.

  16. The social construction of deafblindness

    DEFF Research Database (Denmark)

    Dammeyer, Jesper Herup

    2007-01-01

      At first sight one might think that deafblindness could be determined in a rather straightforward way by objective criteria, e.g., by medical vision and hearing tests. Reality - for most people, who are familiar with deafblindness - as we know, is not like that. But then, what is deafblindenss......? The following short article describes how deafblindness can be understood as a social construction - or maybe rather social practice or a special culture. The message of the article is that we, in our work of improving the offers to deafblind people, may benefit from being aware of the social construction......" rather than a compensation for missing vision and hearing....

  17. Construction of UHVAC Test Base

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The construction of the UHVAC test base of SGCC is an important component of the pilot project on UHVAC power transmission and transformation in China. This paper introduces the layout, technical requirements and main test functions of the UHVAC test base as well as difficulties during construction. It lays stress on the characterization of a 1 000-kV single-circuit test line, a 1 000-kV one-tower double-circuit test line, a full voltage test site for UHV equipment, a climate laboratory, a corona cage and a...

  18. Partnering models in Nordic construction

    DEFF Research Database (Denmark)

    Larsen, Jacob Norvig

    Traditionally, procurement and contractual policies adopted by building and construction clients produce a system in which clients procure design services separately from construction services, while operation and maintenance have been subject to further, separate procurement actions....... This can involve contractual changes, but is more often primarily founded on agreements and commitments outside the contract framework. The paper presents evidence from an explorative study of partnering and partnerships in five Nordic countries. The study was implemented in a collaborative network......, the concept of partnering was used somewhat ambiguously in the Nordic building industries covering roughly any kind of business relation from alliances, consortia, framework agreements to project partnering....

  19. International Construction Disputes in Denmark

    DEFF Research Database (Denmark)

    Cavaleri, Sylvie Cécile

    2016-01-01

    The article discusses whether the very peculiar Danish system of resolution of construction disputes, which is an hybrid between arbitration and State-Court proceedings, is geared for disputes involving non-Danish parties, comes to the conclusion that it is not, and proposes amendments to the sys......The article discusses whether the very peculiar Danish system of resolution of construction disputes, which is an hybrid between arbitration and State-Court proceedings, is geared for disputes involving non-Danish parties, comes to the conclusion that it is not, and proposes amendments...

  20. Construction Technology of Long Span Bridges

    DEFF Research Database (Denmark)

    Gimsing, Niels Jørgen

    2000-01-01

    A large variety of construction methods are used during construction of major bridges, and in many cases the final structural system must be chosen with due respect to the construction process. Today the preferred construction methods are: the free-cantilever method, the launching method and the ......A large variety of construction methods are used during construction of major bridges, and in many cases the final structural system must be chosen with due respect to the construction process. Today the preferred construction methods are: the free-cantilever method, the launching method...

  1. Production of γ-linolenic acid and stearidonic acid by Synechococcus sp. PCC7002 containing cyanobacterial fatty acid desaturase genes

    Science.gov (United States)

    Dong, Xuewei; He, Qingfang; Peng, Zhenying; Yu, Jinhui; Bian, Fei; Li, Youzhi; Bi, Yuping

    2016-07-01

    Genetic modification is useful for improving the nutritional qualities of cyanobacteria. To increase the total unsaturated fatty acid content, along with the ratio of ω-3/ω-6 fatty acids, genetic engineering can be used to modify fatty acid metabolism. Synechococcus sp. PCC7002, a fast-growing cyanobacterium, does not contain a Δ6 desaturase gene and is therefore unable to synthesize γ-linolenic acid (GLA) and stearidonic acid (SDA), which are important in human health. In this work, we constructed recombinant vectors Syd6D, Syd15D and Syd6Dd15D to express the Δ15 desaturase and Δ6 desaturase genes from Synechocystis PCC6803 in Synechococcus sp. PCC7002, with the aim of expressing polyunsaturated fatty acids. Overexpression of the Δ15 desaturase gene in Synechococcus resulted in 5.4 times greater accumulation of α-linolenic acid compared with the wild-type while Δ6 desaturase gene expression produced both GLA and SDA. Co-expression of the two genes resulted in low-level accumulation of GLA but much larger amounts of SDA, accounting for as much to 11.64% of the total fatty acid content.

  2. [Teichoic acids from lactic acid bacteria].

    Science.gov (United States)

    Livins'ka, O P; Harmasheva, I L; Kovalenko, N K

    2012-01-01

    The current view of the structural diversity of teichoic acids and their involvement in the biological activity of lactobacilli has been reviewed. The mechanisms of effects of probiotic lactic acid bacteria, in particular adhesive and immunostimulating functions have been described. The prospects of the use of structure data of teichoic acid in the assessment of intraspecific diversity of lactic acid bacteria have been also reflected.

  3. Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink.

    Science.gov (United States)

    Skardal, Aleksander; Devarasetty, Mahesh; Kang, Hyun-Wook; Seol, Young-Joon; Forsythe, Steven D; Bishop, Colin; Shupe, Thomas; Soker, Shay; Atala, Anthony

    2016-04-21

    Bioprinting has emerged as a versatile biofabrication approach for creating tissue engineered organ constructs. These constructs have potential use as organ replacements for implantation in patients, and also, when created on a smaller size scale as model "organoids" that can be used in in vitro systems for drug and toxicology screening. Despite development of a wide variety of bioprinting devices, application of bioprinting technology can be limited by the availability of materials that both expedite bioprinting procedures and support cell viability and function by providing tissue-specific cues. Here we describe a versatile hyaluronic acid (HA) and gelatin-based hydrogel system comprised of a multi-crosslinker, 2-stage crosslinking protocol, which can provide tissue specific biochemical signals and mimic the mechanical properties of in vivo tissues. Biochemical factors are provided by incorporating tissue-derived extracellular matrix materials, which include potent growth factors. Tissue mechanical properties are controlled combinations of PEG-based crosslinkers with varying molecular weights, geometries (linear or multi-arm), and functional groups to yield extrudable bioinks and final construct shear stiffness values over a wide range (100 Pa to 20 kPa). Using these parameters, hydrogel bioinks were used to bioprint primary liver spheroids in a liver-specific bioink to create in vitro liver constructs with high cell viability and measurable functional albumin and urea output. This methodology provides a general framework that can be adapted for future customization of hydrogels for biofabrication of a wide range of tissue construct types.

  4. Plasma amino acids

    Science.gov (United States)

    Amino acids blood test ... types of methods used to determine the individual amino acid levels in the blood. ... test is done to measure the level of amino acids in the blood. An increased level of a ...

  5. Design and development of two novel constructed wetlands: the duplex-constructed wetland and the constructed wetroof

    NARCIS (Netherlands)

    Zapater Pereyra, M.

    2015-01-01

    Maribel Zapater Pereyra Abstract thesis:  Design and development of two novel constructed wetlands: the Duplex-constructed wetland and the Constructed wetroof Constructed wetlands (CWs) are among the few natural treatment systems that can guarantee an efficient wastewater treatment and an appe

  6. Construction research needs in Europe

    DEFF Research Database (Denmark)

    1991-01-01

    fælles europæisk forsknings- og udviklingsstrategi for byggesektoren har det europæiske netværk af byggeforskningsinstitutter, ENBRI, sammenfattet de nationale forskningstemaer samt analyseret perspektiver og behov. Dette materiale er anvendt som diskussionsoplæg ved symposiet Construction research needs...

  7. A3 TEST STAND CONSTRUCTION

    Science.gov (United States)

    2008-01-01

    THIS IMAGE SHOWS THE DEVELOPMENT AND CONSTRUCTION OF THE A3 TEST STAND IN SUPPORT OF THE ARES/CLV UPPER STAGE ENGINE AT STENNIS SPACE CENTER, MISSISSIPPI. THIS IMAGE IS EXTRACTED FROM A HIGH DEFINITION VIDEO FILE AND IS THE HIGHEST RESOLUTION AVAILABLE.

  8. Clarifying the Construct of Perfectionism

    Science.gov (United States)

    Stairs, Agnes M.; Smith, Gregory T.; Zapolski, Tamika C. B.; Combs, Jessica L.; Settles, Regan E.

    2012-01-01

    The construct of perfectionism is related to many important outcome variables. However, the term "perfectionism" has been defined in many different ways, and items comprising the different existing scales appear to be very different in content. The overarching aim of the present set of studies was to help clarify the specific…

  9. Basic Metal Plate Jewelry Construction.

    Science.gov (United States)

    Petersen, Hugh

    2003-01-01

    Describes how to do an art project with middle school students in which they learn about jewelry making. Explains that the students did not use soldering stations. Discusses the tools used in the project and how the students learned about jewelry construction. (CMK)

  10. ALTERNATE PRICING STRATEGIES IN CONSTRUCTION

    Directory of Open Access Journals (Sweden)

    Krishna Mochtar

    2000-01-01

    Full Text Available Recent research findings on pricing strategies both in general and in construction are reviewed and explored. First%2C pricing strategy in general%2C mostly in the manufacturing industry%2C is reviewed. It includes the concepts of pricing strategy%2C predatory pricing%2C price wars%2C and price policy development. Second%2C pricing strategy in construction is explored. It includes various pricing models for bid price determination%2C such as the Friedman-Gates models%2C expected utility models%2C risk-pricing model%2C and the crew-day%2C multiple regression%2C and fuzzy-set pricing models. In conclusion%2C pricing strategies in construction are still predominantly based on a cost-based approach. More recent models try to close the gap between the models and the real life conditions of a bidder%5C%27s decision-making process. It appears that there are more problems in cost-based pricing as opposed to market-based pricing. Consequently%2C it is highly recommended that%2C alternative pricing approach such as that are closer to the proposed market-based pricing model need to be explored and developed for use in the construction industry. Abstract in Bahasa Indonesia : Pricing+strategy%2C+cost-based+pricing%2C+market-based+pricing.

  11. Discourse Analysis and Social Construction.

    Science.gov (United States)

    Bazerman, Charles

    1990-01-01

    A brief review of social constructivism as a general movement and how it has been applied in particular to scientific knowledge precedes a review of investigations into the role language and linguistic activities have in the social construction of knowledge. A 39-citation unannotated bibliography is included. (CB)

  12. INNOVATION IN THE CONSTRUCTION INDUSTRY

    Directory of Open Access Journals (Sweden)

    David Arditi

    2000-01-01

    Full Text Available In this paper%2C existing innovation models are reviewed. The influence of technology and market-driven schools of thought on the creation and diffusion of innovation is examined. Incremental vs. radical models and autonomous vs. systemic models are considered in the context of the construction industry. The concept of %93technology adoption life cycle%94 is investigated to understand better the acceptance of new technology by key parties. A new model of innovation is proposed. In this model%2C the transformation process of invention into adoption flows through a cyclical mechanism encompassing six phases%3A 1%29 need%2C 2%29 creation%2C 3%29 invention%2C 4%29 innovation%2C 5%29 diffusion%2C and 6%29 adoption. This model clearly shows the interaction of the three most influential domains%3A users%2C changes%2C and the environment. The model also takes into consideration the influence of technology and market-driven forces on the creation and diffusion of innovation. As a consequence of their particular function in the construction activity%2C construction management firms should perform as catalysts in the generation and diffusion of technological advances in the Abstract in Bahasa Indonesia : Innovation%2C+invention%2C+innovation+models%2C+technology+adoption+life+cycle%2C+construction+management.+

  13. Construction of YBS Critical Assembly

    Institute of Scientific and Technical Information of China (English)

    ZHOU; Qi; LIANG; Shu-hong; ZHU; Qing-fu; ZHANG; Wei; YANG; Li-jun; QUAN; Yan-hui

    2015-01-01

    Supported by the XDA program of CAS,the YBS critical assembly has been constructed for the experimental research of the coupling and influence characteristics of spallation target and reactor in ADS system.This work consists of two parts:one is the conversion of the reactor hall and control room,and the other manufacture,installation and commissioning of the critical

  14. The Psychology of Life Construction

    Science.gov (United States)

    Duarte, Maria Eduarda

    2009-01-01

    The present article analyzes, from a historical perspective, the object of the study in the fields of vocational guidance and career management. It then considers the contemporary need to view workers as collaborators within organizations. Finally, in the third part, it presents tentative principles for the construction of a new paradigm called…

  15. Dubin's Minimal Linkage Construct Revisited.

    Science.gov (United States)

    Rogers, Donald P.

    This paper contains a theoretical analysis and empirical study that support the major premise of Robert Dubin's minimal-linkage construct-that restricting communication links increases organizational stability. The theoretical analysis shows that fewer communication links are associated with less uncertainty, more redundancy, and greater…

  16. Knowledge Construction in Critical Ethnography.

    Science.gov (United States)

    Adkins, Amee; Gunzenhauser, Michael G.

    1999-01-01

    Explores the grounding of cultural critique in ethnography as a process of knowledge construction, using concepts from philosophy, anthropology, and sociology of knowledge to identify a theory of knowledge that may inform a postcritical ethnography. The paper proposes a critical ethnography that is more authentic both to its wider social project…

  17. Compositional compiler construction: Oberon0

    NARCIS (Netherlands)

    Viera, Marcos; Swierstra, S. Doaitse

    2015-01-01

    We describe an implementation of an Oberon0 compiler using the techniques proposed in the CoCoCo project. The compiler is constructed out of a collection of pre-compiled, statically type-checked language-definition fragments written in Haskell.

  18. Conversing Life: An Autoethnographic Construction

    Science.gov (United States)

    Hoelson, Christopher N.; Burton, Rod

    2012-01-01

    This autoethnography is a constructed account of a co-exploration into the nature and effects of a longitudinal dyadic conversation process from a relational constructionist perspective. The conversations, between me as participant autoethnographer and a co-participant, aimed at maximising personal learning for both. Through co-created contexts of…

  19. Constructions of Rank Modulation Codes

    CERN Document Server

    Mazumdar, Arya; Zémor, Gilles

    2011-01-01

    Rank modulation is a way of encoding information to correct errors in flash memory devices as well as impulse noise in transmission lines. Modeling rank modulation involves construction of packings of the space of permutations equipped with the Kendall tau distance. We present several general constructions of codes in permutations that cover a broad range of code parameters. In particular, we show a number of ways in which conventional error-correcting codes can be modified to correct errors in the Kendall space. Codes that we construct afford simple encoding and decoding algorithms of essentially the same complexity as required to correct errors in the Hamming metric. For instance, from binary BCH codes we obtain codes correcting $t$ Kendall errors in $n$ memory cells that support the order of $n!/(\\log_2n!)^t$ messages, for any constant $t= 1,2,...$ We also construct families of codes that correct a number of errors that grows with $n$ at varying rates, from $\\Theta(n)$ to $\\Theta(n^{2})$. One of our constr...

  20. Rolloff Roof Observatory Construction (Abstract)

    Science.gov (United States)

    Ulowetz, J. H.

    2015-12-01

    (Abstract only) Lessons learned about building an observatory by someone with limited construction experience, and the advantages of having one for imaging and variable star studies. Sample results shown of composite light curves for cataclysmic variables UX UMa and V1101 Aql with data from my observatory combined with data from others around the world.

  1. ATLAS, Construction of a Giant

    CERN Multimedia

    McCubbin, Norman

    2004-01-01

    One of the most impressive aspects of modern particle physics in the scale of the equipment. The accelerators require the construction of underground tunnels many kilometers long containing evacuated pipes through which beams of particles travel at incredibly high energies. Even more spectacular are the gigantic complex assemblies that make up the experiments themselves

  2. Higher holonomies: comparing two constructions

    DEFF Research Database (Denmark)

    Schaetz, Florian; Arias Abad, Camilo

    2015-01-01

    We compare two different constructions of higher-dimensional parallel transport. On the one hand, there is the two-dimensional parallel transport associated with 2-connections on 2-bundles studied by Baez–Schreiber [2], Faria Martins–Picken [11] and Schreiber–Waldorf [12]. On the other hand, ther...

  3. The Free Universal Construction Kit

    DEFF Research Database (Denmark)

    Stephensen, Jan Løhmann; Hansen, Lone Koefoed

    2013-01-01

    With the increasing economic accessibility of 3D printers, the lessons learned and the logics cultivated on digital Web 2.0 now seems applicable to the world of material things. Released in early 2012 by the artist groups F.A.T. and Sy-lab, the Free Universal Construction Kit is a set of 3D...

  4. Considerations on Writing Test Construction

    Institute of Scientific and Technical Information of China (English)

    王欣

    2005-01-01

    Writing test , wins its popularity in measuring the mastery of one's language ability. In view of the significant role writing playing in a test , some considerations on writing test construction are presented in this paper which anticipates the effective ways for measuring one's complex language ability of application.

  5. The worlds retail buyers construct

    DEFF Research Database (Denmark)

    Esbjerg, Lars

    Previous research on retailer buying has assumed that the context of decision-making is more or less objectively given, or is at least kept constant for the purpose of study. This paper develops an alternative view in which retail buyers and retailers actively participate in the construction...

  6. Parallel Construction of Irreducible Polynomials

    DEFF Research Database (Denmark)

    Frandsen, Gudmund Skovbjerg

    Let arithmetic pseudo-NC^k denote the problems that can be solved by log space uniform arithmetic circuits over the finite prime field GF(p) of depth O(log^k (n + p)) and size polynomial in (n + p). We show that the problem of constructing an irreducible polynomial of specified degree over GF(p) ...

  7. POLYELEOSTEARIC ACID VESICLES

    Institute of Scientific and Technical Information of China (English)

    LI Zichen; XIE Ximng; FAN Qinghua; FANG Yifei

    1992-01-01

    α-Eleostearic acid and β-eleostearic acid formed vesicles in aqueous medium when an ethanol solutionofeleostearic acid was injected rapidly into a vigorously vortexed aqueous phase. Formation of the vesicles was demonstrated by electron microscopic observation and bromothymol blue encapsulation experiments. Polymerizations of the eleostearic acids in the formed vesicles carried out by UV irradiation produced poly-α-eleostearic acid and poly-β-eleostearic acid vesicles.

  8. The ITER project construction status

    Science.gov (United States)

    Motojima, O.

    2015-10-01

    The pace of the ITER project in St Paul-lez-Durance, France is accelerating rapidly into its peak construction phase. With the completion of the B2 slab in August 2014, which will support about 400 000 metric tons of the tokamak complex structures and components, the construction is advancing on a daily basis. Magnet, vacuum vessel, cryostat, thermal shield, first wall and divertor structures are under construction or in prototype phase in the ITER member states of China, Europe, India, Japan, Korea, Russia, and the United States. Each of these member states has its own domestic agency (DA) to manage their procurements of components for ITER. Plant systems engineering is being transformed to fully integrate the tokamak and its auxiliary systems in preparation for the assembly and operations phase. CODAC, diagnostics, and the three main heating and current drive systems are also progressing, including the construction of the neutral beam test facility building in Padua, Italy. The conceptual design of the Chinese test blanket module system for ITER has been completed and those of the EU are well under way. Significant progress has been made addressing several outstanding physics issues including disruption load characterization, prediction, avoidance, and mitigation, first wall and divertor shaping, edge pedestal and SOL plasma stability, fuelling and plasma behaviour during confinement transients and W impurity transport. Further development of the ITER Research Plan has included a definition of the required plant configuration for 1st plasma and subsequent phases of ITER operation as well as the major plasma commissioning activities and the needs of the accompanying R&D program to ITER construction by the ITER parties.

  9. Acid distribution in phosphoric acid fuel cells

    Energy Technology Data Exchange (ETDEWEB)

    Okae, I.; Seya, A.; Umemoto, M. [Fuji Electric Co., Ltd., Chiba (Japan)

    1996-12-31

    Electrolyte acid distribution among each component of a cell is determined by capillary force when the cell is not in operation, but the distribution under the current load conditions had not been clear so far. Since the loss of electrolyte acid during operation is inevitable, it is necessary to store enough amount of acid in every cell. But it must be under the level of which the acid disturbs the diffusion of reactive gases. Accordingly to know the actual acid distribution during operation in a cell is very important. In this report, we carried out experiments to clarify the distribution using small single cells.

  10. 49 CFR 1101.3 - Construction.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 8 2010-10-01 2010-10-01 false Construction. 1101.3 Section 1101.3 Transportation Other Regulations Relating to Transportation (Continued) SURFACE TRANSPORTATION BOARD, DEPARTMENT OF TRANSPORTATION RULES OF PRACTICE DEFINITIONS AND CONSTRUCTION § 1101.3 Construction. The rules of construction contained in chapter 1 of title 1...

  11. 23 CFR 772.19 - Construction noise.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Construction noise. 772.19 Section 772.19 Highways... ABATEMENT OF HIGHWAY TRAFFIC NOISE AND CONSTRUCTION NOISE § 772.19 Construction noise. The following general... may be affected by noise from construction of the project. The identification is to be...

  12. 45 CFR 84.23 - New construction.

    Science.gov (United States)

    2010-10-01

    ... with sections 3-8 of the Uniform Federal Accessibility Standards (UFSA) (appendix A to 41 CFR subpart... 45 Public Welfare 1 2010-10-01 2010-10-01 false New construction. 84.23 Section 84.23 Public... construction. (a) Design and construction. Each facility or part of a facility constructed by, on behalf of,...

  13. 15 CFR 8b.18 - New construction.

    Science.gov (United States)

    2010-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false New construction. 8b.18 Section 8b.18... construction. (a) Design and construction. Each facility or part of a facility constructed by, on behalf of,...

  14. 46 CFR 72.20-15 - Construction.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Construction. 72.20-15 Section 72.20-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS CONSTRUCTION AND ARRANGEMENT Accommodations for Officers and Crew § 72.20-15 Construction. All crew spaces are to be constructed and...

  15. 23 CFR 140.607 - Construction.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Construction. 140.607 Section 140.607 Highways FEDERAL... Bond Issue Projects § 140.607 Construction. Construction shall be supervised by the SHA in the same manner as for regularly financed Federal-aid projects. The FHWA will make construction inspections...

  16. 48 CFR 522.804-2 - Construction.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Construction. 522.804-2... Construction. Goals for the employment of minorities and women in the construction industry are established by... Construction Contractors.” This guide can be accessed at http://www.dol.gov/esa/ofccp/TAguides/ctaguide.htm....

  17. 48 CFR 22.804-2 - Construction.

    Science.gov (United States)

    2010-10-01

    ... applicable to covered geographical areas or projects and (2) applicable requirements of 41 CFR 60-1 and 60-4... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Construction. 22.804-2... Construction. (a) Construction contractors that hold a nonexempt (see 22.807) Government construction...

  18. 38 CFR 18.423 - New construction.

    Science.gov (United States)

    2010-07-01

    ... sections 3-8 of the Uniform Federal Accessibility Standards (USAF) (appendix A to 41 CFR subpart 101-19.6... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false New construction. 18.423... construction. (a) Design and construction. Each facility or part of a facility constructed by, on behalf of,...

  19. On the weak project construction cost management

    Institute of Scientific and Technical Information of China (English)

    高守刚; 姜婧; 李玲

    2013-01-01

    the weak cost management is the most talked about topics in the weak industry, but also the basis of the weak construction business management and focus. With the increasingly fierce market competition, weak construction enterprises, the competition among enterprises wil gradual y transition from product quality competition to price competition. To strengthen the management of the weak construction enterprises cost, cut public spending ef iciency, improve market competitiveness, wil be the main way most weak construction corporate earnings and long-term business strategy. Based on the to weak project construction cost management based on analysis of the type of project construction costs, and further proposed the weak project construction cost management measures.

  20. 花生△12脂肪酸脱氢酶基因高效表达载体构建与原核表达%Construction of a High-efficient Expression Vector of △12 Fatty Acid Desaturase in Peanut and Its Prokaryotical Expression

    Institute of Scientific and Technical Information of China (English)

    殷冬梅; 崔党群; 贾斌

    2007-01-01

    花生是世界范围内广泛种植的重要油料作物之一,其种子中富含油酸和亚油酸.△12脂肪酸脱氢酶(FAD2)是亚油酸合成的关键酶,催化油酸(18:1)在△12位上脱氢生成亚油酸(18:2),但由于△12脂肪酸脱氢酶本身的特性,目前还没有有效的方法将其纯化并在蛋白水平作进一步的研究,尚需对其结构和功能之间以及表达调控进行更深入全面的研究.本文利用从花生中克隆的△12脂肪酸脱氢酶基因(GenBank接受号为AY1006)构建高效表达载体,把花生△12脂肪酸脱氢酶基因全长序列插入到大肠杆菌高效表达载体pRSETB中,构建了pRSET/HO-A融合表达载体,并转化到大肠杆菌表达菌BL21(DE3)pLysS中,在IPTG诱导下,pRSET/HO-A融合表达载体在BL21(DE3)pLysS菌株中高效表达了△12脂肪酸脱氢酶.利用Clon-Tech蛋白纯化Kit进一步分离了目的蛋白,同时加入外源性底物油酸在20℃温育6h后,进行脂肪酸甲酯化处理,通过气相色谱(GC)和气相色谱/质谱(GC-MS)分析表明,所编码的酶具有△12脂肪酸脱氢酶的活性,能将外源性的底物油酸转化为亚油酸,转化率为11.8%.花生△12脂肪酸脱氢酶基因的原核表达目前国内外还未见报导,本实验为其进一步的大量纯化和结构功能分析奠定了基础.%A full-length sequence coding for △12 fatty acid desaturase gene from peanut(Arachis hypogaea L.)was cloned into the expression vector, pRSETB, to generate recombinant plasmid pRSET/HO-A, which was subsequently transformed into expression Escherichia.coli BL21(DE3)pLysS.The △12 fatty acid desaturase was highly expressed in E.coli BL21(DE3)pLysS in the presence of isopropyl-D-thiogalactopyranoside (IPTG).The fusion protein was purified and used to form a reaction system in vitro by adding oleic acid as substrate and incubating it at 20℃ for 6 h.Total fatty acids was extracted and methlesterified and then analyzed with gas chromatography.A novel peak

  1. A Brief Syntactic Research on Resultative Construction

    Institute of Scientific and Technical Information of China (English)

    邱林林

    2008-01-01

    <正>This article will deal with Resultative Construction syntactically.First of all,the Unaccusative Hypothesis will be introduced,and the ideas of different scholars(mainly Levin and Rappaport) towards the question--whether the resultative construction can serve as diagnostic test to tell unergative verbs and unaccusative verbs apart--will be presented.The second focus of the article is about the differences that tell resultative constructions apart from depicitive construction and secondary predication construction.

  2. Experimental studies of Steel Corrugated Constructions

    Directory of Open Access Journals (Sweden)

    Lazarev Yuriy

    2016-01-01

    Full Text Available The purpose of this particular article is to assess existing calculations of steel corrugated constructions. Steel Corrugated Construction is a perspective type of constructions, which is exhibiting numerous advantages in comparison with one that currently applied in automobile and railroad networks (reinforced concrete water-throughput pipes, reinforced concrete frame bridges. The evaluation of experimental data on models of constructions of this particular type has been carried out in order to improve calculations of Steel Corrugated Constructions.

  3. The construction of teaching professionality

    Directory of Open Access Journals (Sweden)

    Heloisa Helena Oliveira de Azevedo

    2013-04-01

    Full Text Available This article was prepared by the results of a research of a investigation about the formative strategies developed by educators related to the construction of the professionality of future childhood and elementary school teachers.From the analysis of the content of the interviews, we want to know what the educators think about the teaching profession and conducting training activities aimed at building the professionalism of future teachers.With theoretical bases for analyses related to the historical-critical conception of education, we inferred that educators have tried to break with the traditional paradigm of teacher’s education, creating new learning alternatives and stimulating the construction of a new professionality that overcomes the culture of isolated an individual thinking

  4. Status of the NCSX construction

    Energy Technology Data Exchange (ETDEWEB)

    Dudek, L. [Princeton Plasma Physics Laboratory (PPPL); Chrzanowski, J. [Princeton Plasma Physics Laboratory (PPPL); Heitzenroeder, P. [Princeton Plasma Physics Laboratory (PPPL); Raftopoulos, S. [Princeton Plasma Physics Laboratory (PPPL); Viola, M. E. [Princeton Plasma Physics Laboratory (PPPL); Neilson, G. [Princeton Plasma Physics Laboratory (PPPL); George, H. [Princeton Plasma Physics Laboratory (PPPL); Rej, D. J. [Princeton Plasma Physics Laboratory (PPPL); Cole, Michael J [ORNL; Goranson, Paul L [ORNL; Freudenberg, Kevin D [ORNL

    2009-06-01

    The National Compact Stellarator Experiment (NCSX) has been under construction at the Princeton Plasma Physics Laboratory (PPPL) in partnership with the Oak Ridge National Laboratory (ORNL). The stellarator core is designed to produce a compact 3D plasma that combines stellarator and tokamak physics advantages. The complex geometry and tight fabrication tolerances of NCSX create some unique engineering and assembly challenges. The NCSX project was cancelled in May 2008; construction activities are presently being phased out in an orderly fashion. This paper will describe the progress of the fabrication and assembly activities of NCSX. Completion of the coil fabrication is on track for the summer of 2008. All three of the vacuum vessel 120 degrees sections have been delivered. Assembly of vacuum vessel services began in May 2006 and is now complete. Assembly of the modular coils into 3-packs for safe storage is presently underway. (C) 2008 Elsevier B.V. All rights reserved.

  5. Status of the NCSX construction

    Energy Technology Data Exchange (ETDEWEB)

    Dudek, Lawrence E.; Chrzanowski, James H.; Heitzenroeder, Philip J.; Raftopoulos, Stephen; Viola, Michael E.; Neilson, George H.; Rej, Donald [Princeton Plasma Physics Laboratory, Princeton, NJ 08543 (United States); Cole, Michael J.; Goranson, Paul; Freudenberg, Kevin [Oak Ridge National Laboratory, Oak Ridge, TN 37831 (United States)

    2009-06-15

    The National Compact Stellarator Experiment (NCSX) has been under construction at the Princeton Plasma Physics Laboratory (PPPL) in partnership with the Oak Ridge National Laboratory (ORNL). The stellarator core is designed to produce a compact 3D plasma that combines stellarator and tokamak physics advantages. The complex geometry and tight fabrication tolerances of NCSX create some unique engineering and assembly challenges. The NCSX project was cancelled in May 2008; construction activities are presently being phased out in an orderly fashion. This paper will describe the progress of the fabrication and assembly activities of NCSX. Completion of the coil fabrication is on track for the summer of 2008. All three of the vacuum vessel 120 deg. sections have been delivered. Assembly of vacuum vessel services began in May 2006 and is now complete. Assembly of the modular coils into 3-packs for safe storage is presently underway.

  6. General Construction of Tubular Geometry

    CERN Document Server

    Mukhopadhyay, Partha

    2016-01-01

    We consider the problem of locally describing tubular geometry around a submanifold embedded in a (pseudo)Riemannian manifold in its general form. Given the geometry of ambient space in an arbitrary coordinate system and equations determining the submanifold in the same system, we compute the tubular expansion coefficients in terms of this {\\it a priori data}. This is done by using an indirect method that crucially applies the tubular expansion theorem for vielbein previously derived. With an explicit construction involving the relevant coordinate and non-coordinate frames we verify consistency of the whole method up to quadratic order in vielbein expansion. Furthermore, we perform certain (long and tedious) higher order computation which verifies the first non-trivial spin connection term in the expansion for the first time. Earlier a similar method was used to compute tubular geometry in loop space. We explain this work in the light of our general construction.

  7. Program Construction by Verifying Specification

    Institute of Scientific and Technical Information of China (English)

    林洪; 陈国良

    1998-01-01

    A program construction method based on Gamma language is proposed.The problem to be solved is specified by first-order predicate logic and a semantic verification program is constructed directly from the specification.Ways for improving efficiency of the program are also studied.The method differs from the one proposed by Manna and Waldinger,where a program is extracted from the proof of the existence of an object meeting the given specification.On the other hand,it also differs from the classical one used for deriving Gamma programs of Banatre and Le metayes,which consists in decomposing the specification into an invariant and a termination conditon.

  8. SYSTEMIC INNOVATION IN CONSTRUCTION: THE

    DEFF Research Database (Denmark)

    Jensen, Jens Stissing; Koch, Christian

    2007-01-01

    This paper offers an evaluation of a best practice program called ‘Best in Construction’ (BiC) which aimed to identify and document cases of successful ICT use in the Danish construction industry. The program is evaluated using two different approaches. The first approach is result-based. This ap......This paper offers an evaluation of a best practice program called ‘Best in Construction’ (BiC) which aimed to identify and document cases of successful ICT use in the Danish construction industry. The program is evaluated using two different approaches. The first approach is result......-based. This approach evaluates the outcome of the program against the main intentions formulated in the tender material. Drawing on systemic innovation theory an alternative evaluative approach is suggested which focuses on the build-up of organizational and institutional resources to support the development...

  9. Green Construction in Building Renovation

    Science.gov (United States)

    Ksit, Barbara; Majcherek, Michał

    2016-06-01

    Modern materials and construction solutions draw more and more attention to ecology and building certification. Among the criteria appearing in revitalization, an important element is bringing plants back into heavily urbanized areas. In its natural form, this is not possible to carry out everywhere, often requiring large amounts of space. Nowadays, however, there are a number of green roofs and green wall systems, allowing "greener" construction without making significant changes in the urban environment. The article includes a presentation and analysis of selected solutions of biological surfaces known as green roofs and green walls, specifying various solutions and their most important features. The case study focuses primarily on material and design solutions, as well as the potential benefits, risks and limitations in their use. Plants structures on the surfaces of vertical and horizontal partitions continue to be a very interesting alternative to take into account when applying for grants, such as LEED or BREEAM certificates.

  10. Constructive version of Boolean algebra

    CERN Document Server

    Ciraulo, Francesco; Toto, Paola

    2012-01-01

    The notion of overlap algebra introduced by G. Sambin provides a constructive version of complete Boolean algebra. Here we first show some properties concerning overlap algebras: we prove that the notion of overlap morphism corresponds classically to that of map preserving arbitrary joins; we provide a description of atomic set-based overlap algebras in the language of formal topology, thus giving a predicative characterization of discrete locales; we show that the power-collection of a set is the free overlap algebra join-generated from the set. Then, we generalize the concept of overlap algebra and overlap morphism in various ways to provide constructive versions of the category of Boolean algebras with maps preserving arbitrary existing joins.

  11. Construct Measurement in Management Research

    DEFF Research Database (Denmark)

    Nielsen, Bo Bernhard

    2014-01-01

    Far too often do management scholars resort to crude and often inappropriate measures of fundamental constructs in their research; an approach which calls in question the interpretation and validity of their findings. Scholars often legitimize poor choices in measurement with a lack of availability...... of better measures and/or that they are simply following existing research in adopting previously published measures without critically assessing the validity, appropriateness, and applicability of such measures in terms of the focal study. Motivated by a recent dialog in Journal of Business Research......, this research note raises important questions about the use of proxies in management research and argues for greater care in operationalizing constructs with particular attention to matching levels of theory and measurement....

  12. Strategic Scenario Construction Made Easy

    DEFF Research Database (Denmark)

    Duus, Henrik Johannsen

    2016-01-01

    insights from the area of strategic forecasting (of which scenario planning is a proper subset) and experiences gained from a recent course in that area to develop a simpler, more direct, hands-on method for scenario construction and to provide several ideas for scenario construction that can be used......Scenario planning is a well-known way to develop corporate strategy by creating multiple images of alternative futures. Yet although scenario planning grew from very hands-on strategy development efforts in the military and from operations research dedicated to solving practical problems, the use...... of scenarios in business has, in many cases, remained a cumbersome affair. Very often a large group of consultants, employees and staff is involved in the development of scenarios and strategies, thus making the whole process expensive in terms of time, money and human resources. In response, this article uses...

  13. SYSTEM OF STANDARTIZATION OF CONSTRUCTION OPERATIONS ARRANGEMENT

    Directory of Open Access Journals (Sweden)

    Oleynik Pavel Pavlovich

    2012-10-01

    Full Text Available In the proposed article, management of construction operations is represented as a multi-level system; it is considered with the framework of projects including new construction, restructuring and overhaul of buildings and structures. The system of management of construction operations is to be composed of the following three constituent parts. They include a construction and assembling entity, project and operations, and a procurement base. Such matters as the quality of construction products, purchase (lease of building machinery and vehicles are incorporated into the level of the construction and assembling entity. The project level comprises such components of construction operations management as pre-construction preparation of a project, methods and forms of construction management, preparatory works, management of construction activities, real-time operations control, construction quality control, etc. The level of operations and the procurement base covers the needs for materials and equipment, their purchase and procurement, as well as the warehouse management. The main elements of the standardization system are identified. Standards of construction operations management are explained, including 1. General Provisions; 2. Preparation and performance of construction and assembling works; 3. New construction. Building site organization; 4. Demolition (dismantling of buildings and structures; 5. Rules of preparation for acceptance and commissioning of completed residential buildings. The prospects for the further development of the system of standardization of construction operations management are outlined

  14. Construction-Paper Puzzle Masterpieces

    Science.gov (United States)

    Vance, Shelly

    2010-01-01

    Creating an appreciation of art history in her junior-high students has always been one of the author's greatest challenges as an art teacher. In this article, the author describes how her eighth-grade students re-created a famous work of art--piece by piece, like a puzzle or a stained-glass window--out of construction paper. (Contains 1 resource.)

  15. Constructal Hypothesis for Mechanical Deformation

    Directory of Open Access Journals (Sweden)

    Atanu Chatterjee

    2012-08-01

    Full Text Available Mild Steel specimen, when subjected to tensile forces shows considerable plastic deformation before fracture. A cross-section of the fractured specimen has the familiar cup – cone form and shows traces of a three – dimensional parabolic geometry. The morphing of the steel specimen from a volume to a point as a spontaneous, entropy producing or energy dispersing process is analysed using the Constructal law.

  16. Generating families and constructible sheaves

    OpenAIRE

    Shende, Vivek

    2015-01-01

    Let $\\Lambda$ be a Legendrian in the jet space of some manifold $X$. To a generating family presentation of $\\Lambda$, we associate a constructible sheaf on $X \\times \\mathbb{R}$ whose singular support at infinity is $\\Lambda$, and such that the generating family homology is canonically isomorphic to the endomorphism algebra of this sheaf. That is, the theory of generating family homology embeds in sheaf theory, and more specifically in the category studied in [STZ]. When $X = \\mathbb{R}$, i....

  17. Profitability Versus Construction Equipment Maintenance

    Science.gov (United States)

    2010-05-01

    E7(/(3+21(180%(5 ,QFOXGHDUHDFRGH XX-05-2010 Master’s Thesis DEC 2009 - MAY 2010 Profitability Versus Construction Equipment Maintenance...optimization of a perceived profit . This project recommends a decision support model that can be used by private and public entities alike to determine the...best fir acquisition method between rent-lease-buy and guidance for profitability optimization. Methods of life cycle cost estimating and decision

  18. Constructive use of holographic projections

    Energy Technology Data Exchange (ETDEWEB)

    Schroer, Bert [Centro Brasileiro de Pesquisas Fisicas (CBPF), Rio de Janeiro, RJ (Brazil); Institut fuer Theoretische Physik der FU, Berlin (Germany)

    2008-07-01

    Revisiting the old problem of existence of interacting models of QFT with new conceptual ideas and mathematical tools, one arrives at a novel view about the nature of QFT. The recent success of algebraic methods in establishing the existence of factorizing models suggests new directions for a more intrinsic constructive approach beyond Lagrangian quantization. Holographic projection simplifies certain properties of the bulk theory and hence is a promising new tool for these new attempts. (author)

  19. CONSTRUCTING ACCOUNTING UNCERTAINITY ESTIMATES VARIABLE

    OpenAIRE

    Nino Serdarevic

    2012-01-01

    This paper presents research results on the BIH firms’ financial reporting quality, utilizing empirical relation between accounting conservatism, generated in created critical accounting policy choices, and management abilities in estimates and prediction power of domicile private sector accounting. Primary research is conducted based on firms’ financial statements, constructing CAPCBIH (Critical Accounting Policy Choices relevant in B&H) variable that presents particular internal control sys...

  20. Home education: Constructions of choice

    Directory of Open Access Journals (Sweden)

    Ruth MORTON

    2010-10-01

    Full Text Available Families who choose to home educate generally do so due to dissatisfaction with schoolbased education. Common perceptions of home educators oscillate between images of the 'tree-hugging hippy' and the 'religious fanatic'. Whilst attempting to go beyond suchstereotypical dichotomies, this paper will examine three very different groupings of home educators and their varying constructions of childhood and the social world, demonstratingthe spectrum between home education as an expression of human rights and of fundamentalism. The first grouping construct home education as a 'natural' choice, often presented in political opposition to existing social structures. For the second grouping home education is predominantly a 'social' choice relating to the conscious transmission of various forms of capital. Finally there are 'last resort' home educators for whom home education is not perceived as a choice. Based on qualitative research, this paper will argue that, even where home education is constructed as natural, the social aspects and impacts of home education choices cannot be ignored.

  1. Utilizing platforms in industrialized construction

    DEFF Research Database (Denmark)

    Bonev, Martin; Wörösch, Michael; Hvam, Lars

    2015-01-01

    Purpose – The purpose of this paper is to explore the development of a platform-based projectexecution in the industrialised construction sector, with a focus on systematically balancing cost andvalue. Offering custom-tailored buildings at reasonable costs has been a growing concern for manyconst......Purpose – The purpose of this paper is to explore the development of a platform-based projectexecution in the industrialised construction sector, with a focus on systematically balancing cost andvalue. Offering custom-tailored buildings at reasonable costs has been a growing concern...... for manyconstruction companies. A promising approach adapted by operations management and design theoryregards individual building projects as the adjustment and recombination of components and processesfrom a set of predefined platforms, while configuration systems assure feasible buildingsolutions. Design....../methodology/approach – After adapting some of the underlying assertions of platformdesign to the engineer-to-order (ETO) situation in construction, the practical implications are evaluatedon a case study of a precast manufacturer using high performance concrete. Findings – Based on empirical findings from three distinct...

  2. Construction of multiform scFv antibodies using linker peptide

    Institute of Scientific and Technical Information of China (English)

    Shihua Wang; Cengjie Zheng; Ying Liu; Huirong Zheng; Zonghua Wang

    2008-01-01

    Multiform single chain variable fragments (acFvs) including different length linker scFvs and bispecific seFv were constructed. The linker lengths of 0, 3, 5, 8, 12, and 15 amino acids between VH and VL of antideoxynivalenol (anti-DON) scFv were used to analyze the affinities of scFvs. The affinity constants of these scFvs increased when the linker was lower than 12 amino acids. The affinity constant would not change when the linker was longer than 12 amino acids. Fusion gene of anti-DON seFv and antizearalenone (anti-ZEN) scFv was also constructed through eormection by a short peptide tinker DNA to express a bispecific scFv. The affinity constants assay showed that the two scFvs of fusion bispecific scFv remained their own affinity compared to their parental scFvs. Competitive direct enzyme linked immunosorbent assay was used to detect DON and ZEN in contaminated wheat (Triticum aestivum L.) samples, and the results indicated that this bispecifie acFv was applicable in DON and ZEN detection. This work confirmed that bispecific scFv could be successfully obtained, and might also have an application in diagnosing fungal infection, and breeding transgertic plants.

  3. Ligand-Enabled γ-C–H Olefination and Carbonylation: Construction of β-Quaternary Carbon Centers

    OpenAIRE

    Li, Suhua; Chen, Gang; Feng, Chen-Guo; Gong, Wei; Yu, Jin-Quan

    2014-01-01

    Monoselective γ-C–H olefination and carbonylation of aliphatic acids has been accomplished by using a combination of a quinoline-based ligand and a weakly coordinating amide directing group. The reaction provides a new route for constructing richly functionalized all-carbon quaternary carbon centers at the β-position of aliphatic acids.

  4. Ligand-enabled γ-C-H olefination and carbonylation: construction of β-quaternary carbon centers.

    Science.gov (United States)

    Li, Suhua; Chen, Gang; Feng, Chen-Guo; Gong, Wei; Yu, Jin-Quan

    2014-04-09

    Monoselective γ-C-H olefination and carbonylation of aliphatic acids has been accomplished by using a combination of a quinoline-based ligand and a weakly coordinating amide directing group. The reaction provides a new route for constructing richly functionalized all-carbon quaternary carbon centers at the β-position of aliphatic acids.

  5. Non-protein amino acids in peptide design

    Indian Academy of Sciences (India)

    S Aravinda; N Shamala; Rituparna S Roy; P Balaram

    2003-10-01

    An overview of the use of non-protein amino acids in the design of conformationally well-defined peptides, based on work from the author’s laboratory, is discussed. The crystal structures of several designed oligopeptides illustrate the use -aminoisobutyric acid (Aib) in the construction of helices, D-amino acids in the design of helix termination segments and DPro-Xxx segments for nucleating of -hairpin structures. - and -amino acid residues have been used to expand the range of designed polypeptide structures.

  6. Construction Managers’ Perception of the Factors Affecting Sustainability in Construction Projects

    Directory of Open Access Journals (Sweden)

    Reza Taheriattar

    2014-07-01

    Full Text Available Sustainable construction is a comprehensive concept which requires long-term planning. Moreover, construction managers play a key role in leading, planning and scheduling of a construction project. As a result, sustainability of construction projects can be affected by construction managers’ decisions. In addition, for greater development of sustainable construction, affecting factors should firstly be notified. Therefore, it seems necessary to investigate construction managers’ perception of the factors affecting sustainability in construction projects. To quantify managers’ perspective of construction sustainability, a survey involving 15 construction managers from Iran construction industry was employed. A statistical comparative analysis used to identify the most important factors affecting sustainability performance at project-level. The results show that most of the factors affecting sustainable construction can be addressed by management teams. The findings will be useful for managers to improve construction sustainability performance at project-level.

  7. Advanced construction management for lunar base construction - Surface operations planner

    Science.gov (United States)

    Kehoe, Robert P.

    1992-01-01

    The study proposes a conceptual solution and lays the framework for developing a new, sophisticated and intelligent tool for a lunar base construction crew to use. This concept integrates expert systems for critical decision making, virtual reality for training, logistics and laydown optimization, automated productivity measurements, and an advanced scheduling tool to form a unique new planning tool. The concept features extensive use of computers and expert systems software to support the actual work, while allowing the crew to control the project from the lunar surface. Consideration is given to a logistics data base, laydown area management, flexible critical progress scheduler, video simulation of assembly tasks, and assembly information and tracking documentation.

  8. Gas-phase Acidities of Aspartic Acid, Glutamic Acid, and their Amino Acid Amides.

    Energy Technology Data Exchange (ETDEWEB)

    Li, Zhong; Matus, Myrna H; Velazquez, Hector A; Dixon, David A; Cassady, Carolyn J

    2007-02-14

    Gas-phase acidities (GA or ΔGacid) for the two most acidic common amino acids, aspartic acid and glutamic acid, have been determined for the first time. Because of the amide linkage’s importance in peptides and as an aid in studying side chain versus main chain deprotonation, aspartic acid amide and glutamic acid amide were also studied. Experimental GA values were measured by proton transfer reactions in an electrospray ionization/Fourier transform ion cyclotron resonance mass spectrometer. Calculated GAs were obtained by density functional and molecular orbital theory approaches. The best agreement with experiment was found at the G3MP2 level; the MP2/CBS and B3LYP/aug-cc-pVDZ results are 3–4 kcal/mol more acidic than the G3MP2 results. Experiment shows that aspartic acid is more acidic than glutamic acid by ca. 3 kcal/mol whereas the G3MP2 results show a smaller acidity difference of 0.2 kcal/mol. Similarly, aspartic acid amide is experimentally observed to be ca. 2 kcal/mol more acidic than glutamic acid amide whereas the G3MP2 results show a correspondingly smaller energy difference of 0.7 kcal/mol. The computational results clearly show that the anions are all ring-like structures with strong hydrogen bonds between the OH or NH2 groups and the CO2- group from which the proton is removed. The two amino acids are main-chain deprotonated. In addition, use of the COSMO model for the prediction of the free energy differences in aqueous solution gave values in excellent agreement with the most recent experimental values for pKa. Glutamic acid is predicted to be more acidic than aspartic acid in aqueous solution due to differential solvation effects.

  9. Aerogenic Vaccination With a Burkholderia mallei Auxotroph Protects Against Aerosol-Initiated Glanders in Mice

    Science.gov (United States)

    2005-03-14

    Greenfield RA. Other bacte- rial diseases as a potential consequence of bioterrorism: Q fever, brucellosis , glanders, and melioidosis. J Okla State Med...sheep and goats . Prev Vet Med 1997;31:275–83. 15] Garmory HS, Griffin KF, Brown KA, Titball RW. Oral immunisation with live aroA attenuated Salmonella

  10. Biosynthesis of biotin from dethiobiotin by the biotin auxotroph Lactobacillus plantarum.

    OpenAIRE

    Bowman, W C; DeMoll, E

    1993-01-01

    Lactobacillus plantarum requires biotin for growth. We show that in the presence of high levels of the biotin biosynthetic precursor, dethiobiotin, L. plantarum synthesizes biotin and grows in medium with dethiobiotin but without biotin. Lactobacillus casei also grew under similar conditions.

  11. Handbook of energy use for building construction

    Energy Technology Data Exchange (ETDEWEB)

    Stein, R.G.; Stein, C.; Buckley, M.; Green, M.

    1980-03-01

    The construction industry accounts for over 11.14% of the total energy consumed in the US annually. This represents the equivalent energy value of 1 1/4 billion barrels of oil. Within the construction industry, new building construction accounts for 5.19% of national annual energy consumption. The remaining 5.95% is distributed among new nonbuilding construction (highways, ralroads, dams, bridges, etc.), building maintenance construction, and nonbuilding maintenance construction. The handbook focuses on new building construction; however, some information for the other parts of the construction industry is also included. The handbook provides building designers with information to determine the energy required for buildings construction and evaluates the energy required for alternative materials, assemblies, and methods. The handbook is also applicable to large-scale planning and policy determination in that it provides the means to estimate the energy required to carry out major building programs.

  12. Sustainable development and construction industry in Malaysia

    Directory of Open Access Journals (Sweden)

    Suliman L. Kh. M.

    2009-12-01

    Full Text Available Sustainable construction is a way for the building and infrastructure industry to move towards achieving sustainable development, taking into account environmental, socioeconomic and cultural issues. Differing approaches and differing economic markets lead to different priorities. This paper presents the construction scenario of Malaysia and the developments in sustainable construction taking place in this country. Barriers to the implementation of sustainable construction are discussed. A list of recommendation was proposed to drive sustainable construction in this country. In conclusion, the status of sustainable construction in Malaysia is still in its infancy. The lack of awareness, training and education, ineffective procurement systems, existing public policies and regulatory frameworks are among the major barriers for sustainable construction in Malaysia. Besides the needs for capacities, technologies and tools, total and ardent commitment by all players in the construction sectors including the governments and the public atlarge are required in order to achieve sustainable construction in Malaysia.

  13. The Effects of Extracellular Matrix on Tissue Engineering Construction of Cartilage in Vitro

    Institute of Scientific and Technical Information of China (English)

    YU Li; LI Fa-tao; TANG Ming-qiao; YAN Wei-qun

    2006-01-01

    The effects of various cartilage extracellular matrix on the construction of rabbit growth plate cartilage tissue in vitro were studied. The results show that collagen, proteoglycan and hyaluronic acid can promote the growth of cultured chondrocytes but the effects of various cartilage extracellular matrix(ECM)on chondrocyte differentiation are different. Collagen can promote the hypertrophy of chondrocytes while proteoglycan and hyaluronic acid inhibit the transition of mature chondrocytes into hypertrophied chondrocytes.

  14. Discussion of Pickling and Rinsing Process for Hydraulic Pipeline Construction%液压管道施工酸洗及冲洗工艺探讨

    Institute of Scientific and Technical Information of China (English)

    师志民

    2015-01-01

    The acid wash construction and construction technology of hydraulic pipe summarize, this article construction unit testing hydraulic pipes pickling,rinse construction quality and construction unit of the hydraulic pipes pickling,rinse construction has a certain reference value.%对施工中液压管道的酸洗及冲洗施工工艺进行总结,此文对建设单位检验液压管道酸洗、冲洗施工质量及施工单位对液压管道酸洗、冲洗施工有一定参考价值。

  15. Gas-phase acidities of aspartic acid, glutamic acid, and their amino acid amides

    Science.gov (United States)

    Li, Zhong; Matus, Myrna H.; Velazquez, Hector Adam; Dixon, David A.; Cassady, Carolyn J.

    2007-09-01

    Gas-phase acidities (GA or [Delta]Gacid) for the two most acidic common amino acids, aspartic acid and glutamic acid, have been determined for the first time. Because of the amide linkage's importance in peptides and as an aid in studying side chain versus main chain deprotonation, aspartic acid amide and glutamic acid amide were also studied. Experimental GA values were measured by proton transfer reactions in an electrospray ionization/Fourier transform ion cyclotron resonance mass spectrometer. Calculated GAs were obtained by density functional and molecular orbital theory approaches. The best agreement with experiment was found at the G3MP2 level; the MP2/CBS and B3LYP/aug-cc-pVDZ results are 3-4 kcal/mol more acidic than the G3MP2 results. Experiment shows that aspartic acid is more acidic than glutamic acid by ca. 3 kcal/mol whereas the G3MP2 results show a smaller acidity difference of 0.2 kcal/mol. Similarly, aspartic acid amide is experimentally observed to be ca. 2 kcal/mol more acidic than glutamic acid amide whereas the G3MP2 results show a correspondingly smaller energy difference of 0.7 kcal/mol. The computational results clearly show that the anions are all ring-like structures with strong hydrogen bonds between the OH or NH2 groups and the CO2- group from which the proton is removed. The two amino acids are main-chain deprotonated. In addition, use of the COSMO model for the prediction of the free energy differences in aqueous solution gave values in excellent agreement with the most recent experimental values for pKa. Glutamic acid is predicted to be more acidic than aspartic acid in aqueous solution due to differential solvation effects.

  16. Improving construction performance through supply chain management

    Institute of Scientific and Technical Information of China (English)

    王要武; 薛小龙

    2004-01-01

    A typical model of construction supply chain (CSC) is presented firstly. Then the characteristics and problems of CSC are identified. The authors also describe the progress towards adoption of supply chain management (SCM) in the construction industry. Traditional construction management approaches (CMAs) are compared with new SCM-based CMAs. The authors suggest that integrated SCM based on cooperating competition is crucial to improve construction performance. Based on relevant results from related research, a framework for integrated construction supply chain management (CSCM) is developed to guide the effective implementation of SCM in construction.

  17. Facility design, construction, and operation

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-04-01

    France has been disposing of low-level radioactive waste (LLW) at the Centre de Stockage de la Manche (CSM) since 1969 and now at the Centre de Stockage de l`Aube (CSA) since 1992. In France, several agencies and companies are involved in the development and implementation of LLW technology. The Commissariat a l`Energie Atomic (CEA), is responsible for research and development of new technologies. The Agence National pour la Gestion des Dechets Radioactifs is the agency responsible for the construction and operation of disposal facilities and for wastes acceptance for these facilities. Compagnie Generale des Matieres Nucleaires provides fuel services, including uranium enrichment, fuel fabrication, and fuel reprocessing, and is thus one generator of LLW. Societe pour les Techniques Nouvelles is an engineering company responsible for commercializing CEA waste management technology and for engineering and design support for the facilities. Numatec, Inc. is a US company representing these French companies and agencies in the US. In Task 1.1 of Numatec`s contract with Martin Marietta Energy Systems, Numatec provides details on the design, construction and operation of the LLW disposal facilities at CSM and CSA. Lessons learned from operation of CSM and incorporated into the design, construction and operating procedures at CSA are identified and discussed. The process used by the French for identification, selection, and evaluation of disposal technologies is provided. Specifically, the decisionmaking process resulting in the change in disposal facility design for the CSA versus the CSM is discussed. This report provides` all of the basic information in these areas and reflects actual experience to date.

  18. INFANTILISM: THEORETICAL CONSTRUCT AND OPERATIONALIZATION

    Directory of Open Access Journals (Sweden)

    Yelena V. Sabelnikova

    2016-01-01

    Full Text Available The aim of the presented research is to define and operationalize theoretically the concept of infantilism and its construct. The content of theoretical construct «infantilism» is analyzed. Methods. The methods of theoretical research involve analysis and synthesis. The age and content criteria are analysed in the context of childhood and adulthood. The traits which can be interpreted as adult infantile traits are described. Results. The characteristics of adult infantilism in modern world taking into account the increasing of information flows and socio-economic changes are defined. The definition of the concept «infantilism» including its main features is given. Infantilism is defined as the personal organization including features and models of the previous age period not adequate for the real age stage with emphasis on immaturity of the emotional and volitional sphere. Scientific novelty. The main psychological characteristics of adulthood are described as the reflection, requirement to work and professional activity, existence of professional self-determination, possession of labor skills, need for selfrealization, maturity of the emotional and volitional sphere. As objective adulthood characteristics are considered the following: transition to economic and territorial independence of a parental family, and also development of new social roles, such as a worker, spouse, and parent. Two options of a possible operationalization of concept are allocated: objective (existence / absence in real human life of objective criteria of adulthood and subjective (the self-report on subjective feeling of existence / lack of psychological characteristics of adulthood. Practical significance consists in a construct operationalization of «infantilism» which at the moment has so many interpretations. That operationalization is necessary for the further analysis and carrying out various researches. 

  19. CONTACT DERMATITIS AMONG CONSTRUCTION WORKERS

    Directory of Open Access Journals (Sweden)

    Ida Ayu Diah Purnama Sari

    2013-02-01

    Full Text Available Contact dermatitis is a form of skin inflammation with spongiosis or intercellular edema of the epidermis due to the interaction of irritants and allergens. While occupational contact dermatitis is an inflammation of the skin due to exposure to irritants or allergens in the workplace. One of the jobs that have a high risk of the disease are construction workers. Although the disease is rarely-threatening but can cause high morbidity and suffering for workers, so it can affect the economy and quality of life of patients.

  20. On constructing disjoint linear codes

    Institute of Scientific and Technical Information of China (English)

    ZHANG Weiguo; CAI Mian; XIAO Guozhen

    2007-01-01

    To produce a highly nonlinear resilient function,the disjoint linear codes were originally proposed by Johansson and Pasalic in IEEE Trans.Inform.Theory,2003,49(2):494-501.In this paper,an effective method for finding a set of such disjoint linear codes is presented.When n≥2k,we can find a set of[n,k] disjoint linear codes with joint linear codes exists with cardinality at least 2.We also describe a result on constructing a set of [n,k] disjoint linear codes with minimum distance at least some fixed positive integer.

  1. Constructing new optimal entanglement witnesses

    CERN Document Server

    Chruscinski, Dariusz; Sarbicki, Gniewomir

    2009-01-01

    We provide a new class of indecomposable entanglement witnesses. In 4 x 4 case it reproduces the well know Breuer-Hall witness. We prove that these new witnesses are optimal and atomic, i.e. they are able to detect the "weakest" quantum entanglement encoded into states with positive partial transposition (PPT). Equivalently, we provide a new construction of indecomposable atomic maps in the algebra of 2k x 2k complex matrices. It is shown that their structural physical approximations give rise to entanglement breaking channels. This result supports recent conjecture by Korbicz et. al.

  2. Reconstruction of Space Steel Constructions

    OpenAIRE

    BRODNIANSKY, Jan; AGOCS, Zoltan; AROCH, Rudolf; SLIVANSKY, Milos

    2010-01-01

    p. 1328-1337 Reconstruction of some special types of constructions - silos, timber roof structure and a winter stadium are described. The silos are cylindrical with a diameter of 36.0m with a total height of 50.5m. The silo shell is made from steel plates of variable thickness. Capacity of the silo is 60 000t. Gradually, during more than 30 years of operation, local deformations and distinctive 6000mm high cracks in the bottom part of the shells of the silos developed. On the basis o...

  3. Real analysis a constructive approach

    CERN Document Server

    Bridger, Mark

    2012-01-01

    A unique approach to analysis that lets you apply mathematics across a range of subjects This innovative text sets forth a thoroughly rigorous modern account of the theoretical underpinnings of calculus: continuity, differentiability, and convergence. Using a constructive approach, every proof of every result is direct and ultimately computationally verifiable. In particular, existence is never established by showing that the assumption of non-existence leads to a contradiction. The ultimate consequence of this method is that it makes sense-not just to math majors but also to students from a

  4. Constructing the E-ELT

    Science.gov (United States)

    de Zeeuw, T.; Tamai, R.; Liske, J.

    2014-12-01

    At its meeting on 3 and 4 December 2014, the ESO Council gave the green light for the construction of the E-ELT in two phases and authorised spending of up to 1012.5 million euros on Phase 1, which will provide a fully working 39-metre telescope with a suite of powerful instruments. It will allow the initial characterisation of earth-mass exoplanets, studies of the resolved stellar populations in nearby galaxies as well as ultrasensitive observations of the deep Universe. This article provides the context for this momentous decision and describes the two-phase approach.

  5. Constructing Outcomes in Teacher Education

    Directory of Open Access Journals (Sweden)

    Marilyn Cochran-Smith

    2001-04-01

    Full Text Available As we enter the twenty-first century, the outcomes, consequences, and results of teacher education have become critical topics in nearly all of the state and national policy debates about teacher preparation and licensure as well as in the development of many of the privately and publicly funded research agendas related to teacher and student learning. In this article, I argue that teacher education reform over the last fifty years has been driven by a series of questions about policy and practice. The question that is currently driving reform and policy in teacher education is what I refer to as "the outcomes question." This question asks how we should conceptualize and define the outcomes of teacher education for teacher learning, professional practice, and student learning, as well as how, by whom, and for what purposes these outcomes should be documented, demonstrated, and/or measured. In this article, I suggest that the outcomes question in teacher education is being conceptualized and constructed in quite different ways depending on the policy, research, and practice contexts in which the question is posed as well as on the political and professional motives of the posers. The article begins with an overview of the policy context, including those reforms and initiatives that have most influenced how outcomes are currently being constructed, debated, and enacted in teacher education. Then I identify and analyze three major "takes" on the outcomes question in teacher education—outcomes as the long-term or general impacts of teacher education, outcomes as teacher candidates' scores on high stakes teacher tests, and outcomes as the professional performances of teacher candidates, particularly their demonstrated ability to influence student learning. For each of these approaches to outcomes, I examine underlying assumptions about teaching and schooling, the evidence and criteria used for evaluation, units of analysis, and consequences for the

  6. Automatic Construction of Finite Algebras

    Institute of Scientific and Technical Information of China (English)

    张健

    1995-01-01

    This paper deals with model generation for equational theories,i.e.,automatically generating (finite)models of a given set of (logical) equations.Our method of finite model generation and a tool for automatic construction of finite algebras is described.Some examples are given to show the applications of our program.We argue that,the combination of model generators and theorem provers enables us to get a better understanding of logical theories.A brief comparison betwween our tool and other similar tools is also presented.

  7. Hydrogels Constructed from Engineered Proteins.

    Science.gov (United States)

    Li, Hongbin; Kong, Na; Laver, Bryce; Liu, Junqiu

    2016-02-24

    Due to their various potential biomedical applications, hydrogels based on engineered proteins have attracted considerable interest. Benefitting from significant progress in recombinant DNA technology and protein engineering/design techniques, the field of protein hydrogels has made amazing progress. The latest progress of hydrogels constructed from engineered recombinant proteins are presented, mainly focused on biorecognition-driven physical hydrogels as well as chemically crosslinked hydrogels. The various bio-recognition based physical crosslinking strategies are discussed, as well as chemical crosslinking chemistries used to engineer protein hydrogels, and protein hydrogels' various biomedical applications. The future perspectives of this fast evolving field of biomaterials are also discussed.

  8. Constructive Sets in Computable Sets

    Institute of Scientific and Technical Information of China (English)

    傅育熙

    1997-01-01

    The original interpretation of the constructive set theory CZF in Martin-Loef's type theory uses the‘extensional identity types’.It is generally believed that these‘types’do not belong to type theory.In this paper it will be shown that the interpretation goes through without identity types.This paper will also show that the interpretation can be given in an intensional type theory.This reflects the computational nature of the interpretation.This computational aspect is reinforced by an ω-Set moel of CZF.

  9. A biosynthetic pathway for hexanoic acid production in Kluyveromyces marxianus.

    Science.gov (United States)

    Cheon, Yuna; Kim, Jun-Seob; Park, Jun-Bum; Heo, Paul; Lim, Jae Hyung; Jung, Gyoo Yeol; Seo, Jin-Ho; Park, Jin Hwan; Koo, Hyun Min; Cho, Kwang Myung; Park, Jin-Byung; Ha, Suk-Jin; Kweon, Dae-Hyuk

    2014-07-20

    Hexanoic acid can be used for diverse industrial applications and is a precursor for fine chemistry. Although some natural microorganisms have been screened and evolved to produce hexanoic acid, the construction of an engineered biosynthetic pathway for producing hexanoic acid in yeast has not been reported. Here we constructed hexanoic acid pathways in Kluyveromyces marxianus by integrating 5 combinations of seven genes (AtoB, BktB, Crt, Hbd, MCT1, Ter, and TES1), by which random chromosomal sites of the strain are overwritten by the new genes from bacteria and yeast. One recombinant strain, H4A, which contained AtoB, BktB, Crt, Hbd, and Ter, produced 154mg/L of hexanoic acid from galactose as the sole substrate. However, the hexanoic acid produced by the H4A strain was re-assimilated during the fermentation due to the reverse activity of AtoB, which condenses two acetyl-CoAs into a single acetoacetyl-CoA. This product instability could be overcome by the replacement of AtoB with a malonyl CoA-acyl carrier protein transacylase (MCT1) from Saccharomyces cerevisiae. Our results suggest that Mct1 provides a slow but stable acetyl-CoA chain elongation pathway, whereas the AtoB-mediated route is fast but unstable. In conclusion, hexanoic acid was produced for the first time in yeast by the construction of chain elongation pathways comprising 5-7 genes in K. marxianus.

  10. Signalling network construction for modelling plant defence response.

    Directory of Open Access Journals (Sweden)

    Dragana Miljkovic

    Full Text Available Plant defence signalling response against various pathogens, including viruses, is a complex phenomenon. In resistant interaction a plant cell perceives the pathogen signal, transduces it within the cell and performs a reprogramming of the cell metabolism leading to the pathogen replication arrest. This work focuses on signalling pathways crucial for the plant defence response, i.e., the salicylic acid, jasmonic acid and ethylene signal transduction pathways, in the Arabidopsis thaliana model plant. The initial signalling network topology was constructed manually by defining the representation formalism, encoding the information from public databases and literature, and composing a pathway diagram. The manually constructed network structure consists of 175 components and 387 reactions. In order to complement the network topology with possibly missing relations, a new approach to automated information extraction from biological literature was developed. This approach, named Bio3graph, allows for automated extraction of biological relations from the literature, resulting in a set of (component1, reaction, component2 triplets and composing a graph structure which can be visualised, compared to the manually constructed topology and examined by the experts. Using a plant defence response vocabulary of components and reaction types, Bio3graph was applied to a set of 9,586 relevant full text articles, resulting in 137 newly detected reactions between the components. Finally, the manually constructed topology and the new reactions were merged to form a network structure consisting of 175 components and 524 reactions. The resulting pathway diagram of plant defence signalling represents a valuable source for further computational modelling and interpretation of omics data. The developed Bio3graph approach, implemented as an executable language processing and graph visualisation workflow, is publically available at http://ropot.ijs.si/bio3graph/and can be

  11. Acid Thunder: Acid Rain and Ancient Mesoamerica

    Science.gov (United States)

    Kahl, Jonathan D. W.; Berg, Craig A.

    2006-01-01

    Much of Mesoamerica's rich cultural heritage is slowly eroding because of acid rain. Just as water dissolves an Alka-Seltzer tablet, acid rain erodes the limestone surfaces of Mexican archaeological sites at a rate of about one-half millimeter per century (Bravo et al. 2003). A half-millimeter may not seem like much, but at this pace, a few…

  12. Corrosion behaviour of construction materials for high temperature water electrolysers

    DEFF Research Database (Denmark)

    Nikiforov, Aleksey

    2010-01-01

    proton exchange membrane (PEM) water electrolysers (HTPEMWE). All samples were exposed to anodic polarisation in 85% phosphoric acid electrolyte solution. Platinum and gold plates were tested for the valid comparison. Steady-state voltammetry was used in combination with scanning electron microscopy......Different types of corrosion resistant stainless steels, Ni-based alloys as well as titanium and tantalum were evaluated as a possible metallic bipolar plate and construction material with respect to corrosion resistance under simulated conditions corresponding to the conditions in high temperature...

  13. Recent developments in the catalytic asymmetric synthesis of alpha- and beta-amino acids.

    Science.gov (United States)

    Ma, Jun-An

    2003-09-22

    The stereoselective synthesis of amino acids is of great importance for the construction of optically active natural products and pharmaceuticals. Apart from enzymes, a broad repertoire of chiral reagents, auxiliaries, and catalysts can be used for the formation of amino acids. Asymmetric reactions using catalytic amounts of chiral molecules provide efficient methods for the generation of optically active proteinogenic and nonproteinogenic amino acids. This minireview collects recent work on catalytic asymmetric synthesis of alpha- and beta-amino acids.

  14. Semiotics of constructed complex systems

    Energy Technology Data Exchange (ETDEWEB)

    Landauer, C.; Bellman, K.L.

    1996-12-31

    The scope of this paper is limited to software and other constructed complex systems mediated or integrated by software. Our research program studies foundational issues that we believe will help us develop a theoretically sound approach to constructing complex systems. There have really been only two theoretical approaches that have helped us understand and develop computational systems: mathematics and linguistics. We show how semiotics can also play a role, whether we think of it as part of these other theories or as subsuming one or both of them. We describe our notion of {open_quotes}computational semiotics{close_quotes}, which we define to be the study of computational methods of dealing with symbols, show how such a theory might be formed, and describe what we might get from it in terms of more interesting use of symbols by computing systems. This research was supported in part by the Federal Highway Administration`s Office of Advanced Research and by the Advanced Research Projects Agency`s Software and Intelligent Systems Technology Office.

  15. Constructal blade shape in nanofluids

    Directory of Open Access Journals (Sweden)

    Bai Chao

    2011-01-01

    Full Text Available Abstract Blade configuration of nanofluids has been proven to perform much better than dispersed configuration for some heat conduction systems. The analytical analysis and numerical calculation are made for the cylinder--shaped and regular-rectangular-prism--shaped building blocks of the blade-configured heat conduction systems (using nanofluids as the heat conduction media to find the optimal cross-sectional shape for the nanoparticle blade under the same composing materials, composition ratio, volumetric heat generation rate, and total building block volume. The regular-triangular-prism--shaped blade has been proven to perform better than all the other three kinds of blades, namely, the regular-rectangular-prism--shaped blade, the regular-hexagonal-prism--shaped blade, and the cylinder--shaped blade. Thus, the regular-triangular-prism--shaped blade is selected as the optimally shaped blade for the two kinds of building blocks that are considered in this study. It is also proven that the constructal cylinder--regular-triangular-prism building block performs better than the constructal regular-rectangular-prism--regular-triangular-prism building block.

  16. DeWitt–Virasoro construction

    Indian Academy of Sciences (India)

    Partha Mukhopadhyay

    2011-03-01

    We study a particular approach for analysing worldsheet conformal invariance for bosonic string propagating in a curved background using Hamiltonian formalism. We work in the Schrödinger picture of a single-particle description of the problem where the particle moves in an infinite-dimensional space. Background independence is maintained in this approach by adopting DeWitt’s (Phys. Rev. 85, 653–661, 1952) coordinate-independent formulation of quantum mechanics. This enables us to construct certain background-independent notion of Virasoro generators, called DeWitt–Virasoro (DWV) generators, and invariant matrix elements of an arbitrary operator constructed out of them in spin-zero representation. We show that the DWV algebra is given by the Witt algebra with additional anomalous terms that vanish for Ricci-flat backgrounds. The actual quantum Virasoro generators should be obtained by first introducing the vacuum state and then normal ordering the DWV generators with respect to that. We demonstrate the procedure in flat and pp-wave backgrounds.

  17. Construction of a Pendentive Grid Crossing Vault

    OpenAIRE

    Palacios Gonzalo, Jose Carlos; Bravo Guerrero, Sandra Cynthia

    2012-01-01

    Nuts & bolts of construction history : culture, technology and society :[proceedings of the Fourth International Congress on Construction History, Paris, 3-7 July 2012. ISBN: 978-2-7084-0929-3 . Vol 1 págs 81 a 88

  18. Validity of Sensory Systems as Distinct Constructs

    OpenAIRE

    2014-01-01

    Confirmatory factor analysis testing whether sensory questionnaire items represented distinct sensory system constructs found, using data from two age groups, that such constructs can be measured validly using questionnaire data.

  19. Seeking Constructive Synergy: Design Science and the Constructive Research Approach

    DEFF Research Database (Denmark)

    Piirainen, Kalle; Gonzalez, Rafael A.

    2013-01-01

    Information systems research and management science create knowledge which can be applied in organizations. Design science specifically aims at applying existing knowledge to solve interesting and relevant business problems and has been steadily gaining support in information systems research....... However, design science is not the only design-oriented framework. Accordingly, this raises the question of whether it is possible to compare the results obtained from different brands of design-oriented research. This paper contributes to answering this question by comparing two research approaches......, enabling mutual learning possibilities and suggesting improvements in transparency and rigor. The objective of this paper is to compare design science research with the constructive research approach. The conclusion is that the two approaches are compatible, save for details in practical requirements...

  20. In situ hydrothermal syntheses, structures and photoluminescent properties of four novel metal-organic frameworks constructed by lanthanide (Ln=Ce(III), Pr(III), Eu(III)) and Cu(I) metals with flexible dicarboxylate acids and piperazine-based ligands

    Energy Technology Data Exchange (ETDEWEB)

    Ay, Burak; Karaca, Serkan [Department of Chemistry, Arts and Science Faculty, Çukurova University, 01330 Adana (Turkey); Yildiz, Emel, E-mail: eeyildiz@cu.edu.tr [Department of Chemistry, Arts and Science Faculty, Çukurova University, 01330 Adana (Turkey); Lopez, Valerie [Department of Chemistry, Syracuse University, Syracuse, NY 13244 (United States); Nanao, Max H. [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9 (France); University Grenoble Alpes-Centre National de la Recherche Scientifique-EMBL Unit of Virus Host-Cell Interactions, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9 (France); Zubieta, Jon [Department of Chemistry, Syracuse University, Syracuse, NY 13244 (United States); Université Grenoble Alpes Laboratoire de Physiologie Cellulaire & Végétale, Institut de Recherches en Technologies et Sciences pour le Vivant, 17 rue des Martyrs, 38054 Grenoble cedex 9 (France)

    2016-01-15

    Four novel metal-organic frameworks,[Cu{sub 2}Cl{sub 2}(pyrz)]{sub n} (1) and (H{sub 2}pip){sub n}[Ln{sub 2}(pydc){sub 4}(H{sub 2}O){sub 2}]{sub n} (Ln=Ce (2), Pr (3) and Eu (4), H{sub 2}pzdc=2,3-pyrazinedicarboxylic acid, pyrz=pyrazine, H{sub 2}pydc=2,6-pyridinedicarboxylic acid, H{sub 2}pip=piperazine) have been synthesized under hydrothermal conditions and characterized by the elemental analysis, ICP, Far IR (FIR), FT-IR spectra, TGA, single crystal X-ray diffraction analysis and powder X-ray diffraction (PXRD). Compound 1 is two-dimensional containing Cl-Cu-Cl sites, while the lanthanide complexes contain one-dimensional infinite Ln–O-Ln chains. All the complexes show high thermal stability. The complexes 1–3 exhibit luminescence emission bands at 584, 598 and 614 nm at room temperature when excited at 300 nm. Complex 4 exhibits bright red solid-state phosphorescence upon exposure to UV radiation at room temperature. - Graphical abstract: Four novel metal-organic frameworks have been synthesized under hydrothermal conditions. Thermal and luminescent properties of the compounds have been investigated.

  1. Sustainable development and construction industry in Malaysia

    OpenAIRE

    2009-01-01

    Sustainable construction is a way for the building and infrastructure industry to move towards achieving sustainable development, taking into account environmental, socioeconomic and cultural issues. Differing approaches and differing economic markets lead to different priorities. This paper presents the construction scenario of Malaysia and the developments in sustainable construction taking place in this country. Barriers to the implementation of sustainable construction are discussed. A li...

  2. Risks Associated with Federal Construction Projects

    Science.gov (United States)

    2011-06-01

    Practices Act. Areas of Application. Existing research shows that risk in contract cover a wide range of application in construction. Erikson ...Year) Area of Application Synopsis Erikson and O’Connor (1979) Firm fixed-price construction contracts Determined the cost effects of varying risk...2001, Erikson , C. A. & O’Connor, M. J. (1979). Construction Contract Risk Assignment, Technical Report P-101, Report No. CERL-TR-P-101, Construction

  3. Construction of LuxS gene deletion of Streptococcus mutans and evaluation of the acid tolerance of the mutant%变异链球菌LuxS基因缺陷株的建立及其耐酸能力的研究

    Institute of Scientific and Technical Information of China (English)

    韩福胜; 韩玉植; 刘宇霞

    2010-01-01

    Objective To construct Streptococcus mutans UA159 mutants with deletion of LuxS gene related to quorum-sensing pathway and evaluate the aciduricity of the mutants. Methods Using S. mutans UA159 as materials, the PCR fragments of the upstream and downstream regions of LuxS and erythromycin resistance(Eymr) gene of PJT10 were cloned into plasmid PUC19. The resulting constructs were integrated into the chromosome of S. mutans. LuxS gene deletion mutant was then constructed in S. mutans by means of allelic exchange and selected for resistance to erythromycin. The aciduric ability of the mutant under different pH was measured and S. mutans UA159 was used as control. Results The LuxS-deleted status of S. mutans mutants were confirmed by various PCR and DNA sequencing. The results showed that Eymr gene take the place of LuxS gene, while the mutant can not induce bioluminescenece. The LuxS mutant strain displayed a decreased growth ability with the decreasing pH values compared to those of the wild-type strain UA159. Conclusion A LuxS-negative mutants of S. mutans is constructed. The LuxS quorum sensing system is involved in the regulation of aciduricity of S. mutans UA159.%目的 建立LuxS基因缺失的变异链球菌突变菌株,并对突变株的耐酸能力进行研究.方法 以变异链球菌UA159为材料,运用基因重组方法将红霉素抗性基因(Eymr)与LuxS基因上下游区域的2个基因片段按一定顺序重组到质粒载体PUC19的多克隆位点中,获得了具有红霉素抗性的重组质粒,将载体质粒转化到含完整LuxS基因的变异链球菌UA159中,利用红霉素抗性筛选出LuxS基因缺失的突变株.检测变异链球菌LuxS基因突变菌株在不同pH环境下生长情况,并以正常菌株为对照.结果 PCR基因扩增结果显示,突变株LuxS基因已被Eymr基因完全替换,不能再编码合成AI-2(autoinducer 2)信号分子,扩增产物经DNA测序证实筛选得到了LuxS基因缺失的突变株,并

  4. Method of constructing an improved electrochemical cell

    Science.gov (United States)

    Grimes, Patrick G.; Einstein, Harry

    1984-10-09

    An electrochemical cell construction features a novel co-extruded plastic electrode in an interleaved construction with a novel integral separator-spacer. Also featured is a leak and impact resistant construction for preventing the spill of corrosive materials in the event of rupture.

  5. 48 CFR 936.7101 - Construction contracts.

    Science.gov (United States)

    2010-10-01

    ... CATEGORIES OF CONTRACTING CONSTRUCTION AND ARCHITECT-ENGINEER CONTRACTS Inspection and Acceptance 936.7101 Construction contracts. (a) Inspection services may be performed by the architect-engineer responsible for the design. Inspection services may not be procured from a construction contractor with respect to its...

  6. 23 CFR 710.311 - Construction advertising.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Construction advertising. 710.311 Section 710.311...-WAY AND REAL ESTATE Project Development § 710.311 Construction advertising. The State must manage real... advertising for construction, the State shall develop ROW availability statements and certifications...

  7. Partially Correct Constructs Illuminate Students' Inconsistent Answers

    Science.gov (United States)

    Ron, Gila; Dreyfus, Tommy; Hershkowitz, Rina

    2010-01-01

    We present a view of knowledge construction processes, focusing on partially correct constructs. Motivated by unexpected and seemingly inconsistent quantitative data based on the written reports of students working on an elementary probability task, we analyze in detail the knowledge construction processes of a representative student. We show how…

  8. 46 CFR 167.20-1 - Construction.

    Science.gov (United States)

    2010-10-01

    ... acceptable. (a) American Bureau of Shipping “Rules for Building and Classing Steel Vessels” regarding the... 46 Shipping 7 2010-10-01 2010-10-01 false Construction. 167.20-1 Section 167.20-1 Shipping COAST... Requirements, Construction and Arrangement of Nautical School Ships § 167.20-1 Construction. Except...

  9. Construction Blue Print in Global Market

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Talking of China Communications Construction Company Ltd.(CCCC), we can easily count several national and even world records. It's the largest port construction and design enterprise in China, the team leader in road and bridge construction and design,the biggest in China also among the world top three companies in dredging,and the No.1 container crane manufacturer in the world.

  10. 29 CFR 1911.10 - Construction standards.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Construction standards. 1911.10 Section 1911.10 Labor... STANDARDS Commencement of Rulemaking § 1911.10 Construction standards. The Assistant Secretary may promulgate, modify, or revoke a standard applicable to employments in construction work, as defined in §...

  11. 46 CFR 160.171-9 - Construction.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Construction. 160.171-9 Section 160.171-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Immersion Suits § 160.171-9 Construction. (a) General....

  12. Construction Grammars: Towards a Pedagogical Model

    Science.gov (United States)

    Holme, Randal

    2010-01-01

    "Constructions" are the central unit of grammatical analysis in cognitive linguistics. In formal linguistics "construction" referred to forms that were projected from lexical items rather than from an autonomous syntax. Thus, an expression, "I danced the night away" requires an intransitive verb in a transitive construction provided "away" is…

  13. 45 CFR 605.23 - New construction.

    Science.gov (United States)

    2010-10-01

    ... Standards (USAF) (appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... 45 Public Welfare 3 2010-10-01 2010-10-01 false New construction. 605.23 Section 605.23 Public... Accessibility § 605.23 New construction. (a) Design and construction. Each facility or part of a...

  14. 46 CFR 108.235 - Construction.

    Science.gov (United States)

    2010-10-01

    ... limit drop test in 14 CFR 29.725, or a load of not less than 75 percent of the helicopter maximum weight... 46 Shipping 4 2010-10-01 2010-10-01 false Construction. 108.235 Section 108.235 Shipping COAST... Construction and Arrangement Helicopter Facilities § 108.235 Construction. (a) Each helicopter deck must...

  15. 45 CFR 1151.23 - New construction.

    Science.gov (United States)

    2010-10-01

    ... 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of this section with respect... 45 Public Welfare 3 2010-10-01 2010-10-01 false New construction. 1151.23 Section 1151.23 Public... Prohibited Accessibility § 1151.23 New construction. (a) Design, construction, and alteration. New...

  16. 46 CFR 160.017-13 - Construction.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Construction. 160.017-13 Section 160.017-13 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Chain Ladder § 160.017-13 Construction. (a) General. Each...

  17. 48 CFR 53.236-1 - Construction.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Construction. 53.236-1... AND FORMS FORMS Prescription of Forms 53.236-1 Construction. The following forms are prescribed, as stated below, for use in contracting for construction, alteration, or repair, or dismantling,...

  18. 46 CFR 161.012-7 - Construction.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Construction. 161.012-7 Section 161.012-7 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL ELECTRICAL EQUIPMENT Personal Flotation Device Lights § 161.012-7 Construction....

  19. 46 CFR 160.047-4 - Construction.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Construction. 160.047-4 Section 160.047-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS... and Child § 160.047-4 Construction. (a) General. This specification covers buoyant vests...

  20. 23 CFR 645.115 - Construction.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Construction. 645.115 Section 645.115 Highways FEDERAL... Relocations, Adjustments, and Reimbursement § 645.115 Construction. (a) Part 635, subpart B, of this title, Force Account Construction (justification required for force account work), states that it is...