WorldWideScience

Sample records for abomasum

  1. Computed tomography of the abdomen in Saanen goats: II. liver, spleen, abomasum, and intestine

    OpenAIRE

    2011-01-01

    This study describes the results of computed tomography (CT) of the liver, spleen, abomasum, small intestine and large intestine in 30 healthy Saanen goats. CT examination and anatomical slice preparation postmortem were performed as described in the first communication. After subjective evaluation of the CT images, various variables including the length/size, volume and density of the liver, spleen and gallbladder, the wall thickness of the abomasum, small intestine and large intestine and t...

  2. Characteristics of fluid composition of left displaced abomasum in beef cattle fed high-starch diets.

    Science.gov (United States)

    Ichijo, Toshihiro; Satoh, Hiroshi; Yoshida, Yuki; Murayama, Isao; Kikuchi, Tomoko; Sato, Shigeru

    2014-08-01

    To clarify the pathophysiology of left displaced abomasum (LDA), beef cattle fed high-starch diets were examined. The abomasal pH in beef cattle with LDA was lower than that in non-LDA reference animals (data from beef cattle at an abattoir), suggesting that it facilitated acidity. Bacteriological examinations of the abomasal fluid in cattle with LDA revealed the presence of Pseudomonas spp., Clostridium spp. and Candida spp., presumably reflecting the accelerated influx of ruminal fluid into the abomasum. Biochemical analyses of serum revealed that LDA cattle had higher lactic acid and lower vitamin A and E levels than non-LDA reference animals. These results indicate that beef cattle with LDA may suffer from vitamin A and E deficiencies due to maldigestion of starch and the high acidity of abomasal fluid.

  3. Modification of abomasum contractility by flavonoids present in ruminants diet: in vitro study.

    Science.gov (United States)

    Mendel, M; Chłopecka, M; Dziekan, N; Karlik, W

    2016-09-01

    Flavonoid supplementation is likely to be beneficial in improving rumen fermentation and in reducing the incidence of rumen acidosis and bloat. Flavonoids are also said to increase the metabolic performance during the peripartum period. Ruminants are constantly exposed to flavonoids present in feed. However, it is not clear if these phytochemicals can affect the activity of the gut smooth muscle. Therefore, the aim of the study was to verify the effect of three flavonoids on bovine isolated abomasum smooth muscle. The study was carried out on bovine isolated circular and longitudinal abomasal smooth muscle specimens. All experiments were conducted under isometric conditions. The effect of apigenin, luteolin and quercetin (0.001 to 100 µM) was evaluated on acetylcholine-precontracted preparations. The effect of multiple, but not cumulative, treatment and single treatment with each flavonoid on abomasum strips was compared. Apigenin (0.1 to 100 µM) dose-dependently showed myorelaxation effects. Luteolin and quercetin applied in low doses increased the force of the ACh-evoked reaction. However, if used in high doses in experiments testing a wide range of concentrations, their contractile effect either declined (luteolin) or was replaced by an antispasmodic effect (quercetin). Surprisingly, the reaction induced by flavonoids after repeated exposure to the same phytochemical was not reproducible in experiments testing only single exposure of abomasum strips to the same flavonoid used in a high concentration. Taking into account the physicochemical properties of flavonoids, this data suggests the ability of flavonoids to interfere with cell membranes and, subsequently, to modify their responsiveness. Assuming ruminant supplementation with luteolin or quercetin or their presence in daily pasture, a reduction of the likelihood of abomasum dysmotility should be expected.

  4. Factors influencing the digestion of dietary carbohydrates between the mouth and abomasum of steers.

    Science.gov (United States)

    McAllan, A B; Smith, R H

    1983-09-01

    Six protozoa-free steers with simple rumen and abomasal cannulas were given basal diets consisting of a concentrate mixture of flaked maize and tapioca with either barley straw (BS) or alkali-treated barley straw (BSA). Other diets used were supplemented with urea (BSU and BSAU respectively) or contained fish meal in place of tapioca BSF and BSAF respectively). The diets were given in a 6 X 6 Latin square design. Diets were isoenergetic and provided sufficient metabolizable energy (ME) to support a growth rate of approximately 0.5 kg/d. Basal diets, urea- and fish-meal-supplemented diets had estimated rumen-degradable nitrogen (RDN):ME values (g/MJ) of 0.5, 1.2 and 0.8 respectively. 103Ruthenium and polyethylene glycol were given as flow markers, and flows (g/24 h) at the abomasum of organic matter (OM) and carbohydrate components were calculated. True digestibility coefficients of OM between mouth and abomasum were significantly greater for diets containing alkali-treated straw (approximately 0.63) than for those containing untreated straw (approximately 0.55) but were not significantly affected by N supplementation. Digestibility coefficients of the neutral-sugar components of dietary polysaccharides between mouth and abomasum were 0.28, 0.34, 0.31, 0.23, 0.31 and 0.87 for mannose, galactose arabinose, xylose, cellulose-glucose and starch-glucose respectively for diet BS. Corresponding values were 0.37, 0.42, 0.56, 0.51, 0.40 and 0.88 for diet BSA. All but the mannose and starch-glucose values were significantly greater for the latter diet. N supplementation also led to increases in digestibility of all neutral sugars except mannose and starch-glucose. Fish meal produced a markedly greater effect than urea but only significantly so for cellulose-glucose. Thus, the highest digestibilities were seen for diet BSAF and were 0.68, 0.67, 0.74 and 0.64 for galactose, arabinose, xylose and cellulose-glucose respectively. Of all these sugars xylose consistently showed the

  5. Computed tomography of the abdomen of calves during the first 105 days of life: I. Reticulum, rumen, omasum and abomasum.

    Science.gov (United States)

    Braun, U; Schnetzler, C; Ohlerth, S; Hatz, L; Augsburger, H

    2014-05-01

    Computed tomographic (CT) images of the reticulum, rumen, omasum and abomasum of five healthy Holstein-Friesian bull calves were compared with anatomical transverse cadaver sections of the same calves. The calves were scanned in the transverse plane from the 5th thoracic vertebra to the sacrum six times three weeks apart from birth to 105 days of age. Multiplanar reconstruction was used to create images in sagittal and dorsal planes. After subjective assessment of various anatomical structures, the rumen, omasum and abomasum as well as the ruminal strata (gas cap, fibre mat and fluid phase) were measured. After the last CT scan, all calves were euthanised, and four were kept at -18 °C in sternal position for 14 days. Transverse sections 1.0 to 1.5 cm thick were made from two calves and dorsal and sagittal sections were made from one calf each using a band saw. The CT images and anatomical slices were compared and the structures on the CT images identified. Very clear CT images were obtained from the reticulum, rumen, omasum and abomasum and there was excellent agreement between images and anatomical slices.

  6. Identification and location of the cocaine and amphetamine regulated transcript (CART) in the abomasum of cattle.

    Science.gov (United States)

    Janiuk, Izabela; Młynek, Krzysztof; Wysocki, Jarosław

    2013-05-01

    The cocaine and amphetamine regulated transcript (CART) belongs to the group of peptides with anorexigenic properties and is present in many areas of the central and peripheral nervous systems of numerous mammalian species. Research has suggested an effect on the feeling of appetite and satiety; however, there are no clear clues as to the role of CART in specific organs, including the stomach. Considering the specificity of cattle feeding and digestion, CART may play a highly significant role possibly associated with the option of administering greater amounts of high-volume feeds. Based on the results of immunohistochemical staining of abomasum samples prepared from hybrid bulls, the presence of CART-positive structures and CART distribution were determined in the mucosa, submucosa and muscularis layers of the stomach. Abundant sites of CART were found in the myenteric plexus, nerve fibers innervating the myocytes of the myenteron, neuroendocrine cells of the diffuse neuroendocrine system and the submucous plexus. The preliminary stage of abomasal CART detection suggests that CART is an agent that strongly affects the regulation of motor activity involved in stomach emptying and in secretory functions of the stomach. However, further research is necessary to explain the relationship.

  7. Peritoneal inflammatory response to surgical correction of left displaced abomasum using different techniques.

    Science.gov (United States)

    Wittek, T; Fürll, M; Grosche, A

    2012-12-08

    The objective of this study was to compare the inflammatory response within the abdominal cavity between three surgical methods. The study comprised 45 cows with left displacement of the abomasum, which were allocated into three groups (n = 15). Right flank laparotomy and omentopexy (group R), left flank laparotomy and omentopexy (group L), and laparoscopic abomasopexy (group J) have been applied. Laparoscopic abomasopexy was the only technique that requires perforation of the abomasal wall. Blood and peritoneal fluid (PF) samples were obtained before, and on days 1, 2 and 3 after surgery. Macroscopic and microscopic evaluation of PF were performed. Cytological and biochemical parameters were analysed in blood and PF. No bacteria were present in PF after surgery. The number of PF leukocytes increased in all groups on day 1 after surgery with the highest value after laparoscopy (median, 1st quartile, 3rd quartile, R: 13.1, 6.4, 16.0; L: 13.6, 9.9, 17.4; J: 33.7, 21.1, 46.9 G/l). Laparotomy resulted in an increase of blood and PF CK on day 1 after surgery, whereas, laparoscopy caused an increased PF CK only. All groups had elevated PF D-dimer concentrations before surgery, with further increase in groups R and L on day 1 after surgery.

  8. Blood calcium and phosphorus concentrations in cows with left displaced abomasum

    Directory of Open Access Journals (Sweden)

    Šamanc Horea

    2009-01-01

    Full Text Available Abomasal displacement is one of the most important disorders of the digestive tract in high-yield dairy cows. The etiology and pathogenesis of its occurrence has not been fully clarified to this day. The work examines calcaemia and phosphataemia in cows during the antepartal and postpartal periods and their connection with the incidence of abomasal displacement. The experiment covered 30 cows in advanced stages of gravidity. Blood samples were taken two weeks before expected parturition and two weeks after calving. In all blood samples (taken antepartal and postpartal, concentrations of calcium and an organic phosphorus were determined using a commercial test package (Bio-Merieux. Following parturition, left displacement of the abomasums was established in seven (23.33 % of the 30 cows covered by the experiment (experimental group. The remaining 23 cows in the experiment were clinically healthy, they were placed in a group and represented control animals. The average value of calcaemia for the control group of cows during the antepartal period was 2.72±0.25 mmol/l, and of phosphataemia 2.04±0.25 mmol/l. In the same cows postpartum, average calcaemia values were 2.46±0.22 mmol/l and phosphataemia 1.85±0.29 mmol/l. The average antepartal value for calcaemia in the experimental group of cows was 2.51±0.25 mmol/l, and for phosphataemia 1.73± 0.22 mmol/l. The average values for this group of cows postpartally was 2.13±0.31 mmol/l for calcaemia and 1.43±0.24 mmol/l for phosphataemia. The differences between the concentration values for calcium and phosphorus obtained postpartally between the experimental and control groups were statistically significant. .

  9. Nématodes de l'abomasum du dromadaire au Maroc : une enquête épidémiologique

    OpenAIRE

    Coudray, Alexandre

    2006-01-01

    Les nématodes de l'abomasum sont responsables de lourdes pertes économiques pour les élevages camelins marocains. Peu d'études ont été réalisées dans la région et la présence de troupeau mixte (ovin, caprins et camelins) rend possible la présence d'infestations hétérologues. Les objectifs de l'étude sont de recenser la faune abomasale du dromadaire et d'évaluer l'importance des infestations hétérologues à Haemonchus. Les résultats de l'étude menée pendant un mois à l'abattoir de Lâayoune ont ...

  10. Milk yield and survival of Holstein-Friesian dairy cattle after laparoscopic correction of left-displaced abomasum.

    Science.gov (United States)

    Jorritsma, R; Westerlaan, B; Bierma, M P R; Frankena, K

    2008-06-07

    The milk yield and survival of 91 Holstein-Friesian dairy cows that had had a left-displaced abomasum (LDA) corrected laparoscopically were compared with those of 193 control cows matched for herd, parity and calving date. Ninety per cent of the LDA treatments were performed within four weeks after calving. The risk of being culled during the whole observational period of at least three years was 1.5 times greater for the LDA cows than for their matched herdmates (P<0.01). The risk of being culled in the current lactation was 1.8 times greater for the LDA cows (P=0.01), but risk of being culled after the next calving following the LDA correction was similar for both groups. For the lactation in which the LDA was corrected, there was no difference in the 305-day milk production of 80 of the LDA cows and 182 of the matched cows; however, the mean interval from calving to first service during the same lactation was longer for the LDA cows (115 v 98 days) and the mean calving interval was also longer (451 v 418 days).

  11. Metabolic Profile and Inflammatory Responses in Dairy Cows with Left Displaced Abomasum Kept under Small-Scaled Farm Conditions

    Directory of Open Access Journals (Sweden)

    Fenja Klevenhusen

    2015-10-01

    Full Text Available Left displaced abomasum (LDA is a severe metabolic disease of cattle with a strong negative impact on production efficiency of dairy farms. Metabolic and inflammatory alterations associated with this disease have been reported in earlier studies, conducted mostly in large dairy farms. This research aimed to: (1 evaluate metabolic and inflammatory responses in dairy cows affected by LDA in small-scaled dairy farms; and (2 establish an Animals 2015, 5 1022 association between lactation number and milk production with the outcome of metabolic variables. The cows with LDA had lower serum calcium (Ca, but greater concentrations of non-esterified fatty acids (NEFA and beta-hydroxy-butyrate (BHBA, in particular when lactation number was >2. Cows with LDA showed elevated levels of aspartate aminotransferase, glutamate dehydrogenase, and serum amyloid A (SAA, regardless of lactation number. In addition, this study revealed strong associations between milk yield and the alteration of metabolic profile but not with inflammation in the sick cows. Results indicate metabolic alterations, liver damage, and inflammation in LDA cows kept under small-scale farm conditions. Furthermore, the data suggest exacerbation of metabolic profile and Ca metabolism but not of inflammation and liver health with increasing lactation number and milk yield in cows affected by LDA.

  12. Effects of level of dietary intake and physical form of protein supplement on the digestibilities of different dietary carbohydrates between mouth and abomasum of young steers.

    Science.gov (United States)

    McAllan, A B; Smith, R H

    1983-09-01

    Steers fitted with simple rumen and abomasal cannulas were given isoenergetic diets of rolled barley and chopped straw, pelleted together with some tapioca alone (B) or with some tapioca replaced by coarse soyabean meal (M) or finely ground soyabean flour (F). The diets were given at two levels to support 0.5 (L) and 1.0 (H) kg/d live weight gain. Chromic oxide and PEG were given as digesta flow markers. Mouth to abomasum digestibilities of different dietary sugars at the low level of intake (LB) were 0.65, 0.68, 0.59, 0.56 and 0.94 for arabinose, galactose, xylose, cellulose-glucose and starch-glucose respectively. Corresponding values at the higher level of intake (HB) were 0.55, 0.66, 0.55, 0.44 and 0.93 respectively. Supplementation with either soya bean meal or flour had no significant effects on the mouth to abomasum of dietary carbohydrate digestibilities at either level of feeding.

  13. Pathway of programmed cell death and oxidative stress induced by β-hydroxybutyrate in dairy cow abomasum smooth muscle cells and in mouse gastric smooth muscle.

    Directory of Open Access Journals (Sweden)

    Wulin Tian

    Full Text Available The administration of exogenous β-hydroxybutyrate (β-HB, as well as fasting and caloric restriction, is a condition associated with β-HB abundance and decreased appetite in animals. Increased β-HB and decreased appetite exist simultaneously in some diseases, such as bovine left displaced abomasums (LDA and human chronic gastritis. However, the effects of β-HB on stomach injuries have not been explored. To elucidate the possible effects of exogenous β-HB on the stomach, mice were injected intraperitoneally with β-HB, and bovine abomasum smooth muscle cells (BSMCs were treated with different concentrations of β-HB. We found that β-HB induced BSMCs endoplasmic reticulum- and mitochondria-mediated apoptotic cell death. β-HB promoted Bax expression and caspase-12, -9, and -3 activation while blocking Bcl-2 expression. β-HB also promoted AIF, EndoG release and p53 expression. β-HB acted on key molecules in the apoptotic cell death pathway and increased p38 and c-June NH2-terminal kinase phosphorylation while inhibiting ERK phosphorylation and PCNA expression. β-HB upregulated P27 and P21 mRNA levels while downregulating cyclin and CDK mRNA levels, arresting the cell cycle. These results suggest that BSMCs treated with β-HB can induce oxidative stress, which can be prevented by intracellular calcium chelators BAPTA/AM but not antioxidant NAC. Additionally, these results suggest that β-HB causes ROS generation through a Ca2+-dependent mechanism and that intracellular Ca2+ levels play a critical role in β-HB -induced apoptotic cell death. The impact of β-HB on programmed cell death and oxidative stress in vivo was confirmed in murine experiments. For the first time, we show oxidative stress effects of β-HB on smooth muscle. We propose that β-HB is a possible cause of some stomach diseases, including bovine LDA.

  14. Nitrogen balance in lambs fed low-quality brome hay and infused with differing proportions of casein in the rumen and abomasum.

    Science.gov (United States)

    Swanson, K C; Freetly, H C; Ferrell, C L

    2004-02-01

    Twenty wether lambs (46 +/- 2 kg) fitted with ruminal and abomasal infusion catheters were used in a completely randomized design to determine the effects of differing proportions of ruminal and abomasal casein infusion on N balance in lambs fed low-quality brome hay (0.8% N, DM basis) for ad libitum intake. Wethers were infused with 0 (control) or 10.7 g/d of N from casein with ratios of ruminal:abomasal infusion of 100:0 (100R:0A), 67:33 (67R:33A), 33:67 (33R:67A), or 0:100% (0R:100A), respectively, over a 12-d period. Total N supply (hay N intake + N from casein infusion) was greater (P = 0.001) in lambs receiving casein infusion than in controls. Urinary N excretion (g/d) was greater (P = 0.001) in lambs receiving casein infusion than in controls. Urinary N excretion decreased as casein infusion was shifted from 100R:0A to 33R:67A and then slightly increased in lambs receiving 0R:100A (quadratic, P = 0.02). Total N excretion was greater (P = 0.001) in lambs receiving casein infusion than in controls and decreased linearly (P = 0.005) as casein infusion was shifted to the abomasum. Retained N (g/d, % of N intake, and % of digested N) was greater (P = 0.001) in lambs receiving casein than in controls. Retained N increased as infusion was shifted from 100R:0A to 33R:67A and then slightly decreased in lambs receiving 0R: 100A (quadratic, P infusion to maximize N retention was 68% into the abomasum. The regression suggests that supplementation with undegradable intake protein had an additional benefit over supplementation with ruminally degradable intake protein (100R:0A) and that changing the percentage of ruminally undegradable intake protein in supplemental protein from 33 to 100% resulted in minimal differences in N retention. Apparent N, DM, OM, and energy digestibility (% of intake) was greater (P infused with casein than controls but did not differ among casein infusion groups. These data suggest that feeding protein supplements containing a portion (greater

  15. An evaluation of protein/fat ratio in first DHI test milk for prediction of subsequent displaced abomasum in dairy cows.

    Science.gov (United States)

    Geishauser, T D; Leslie, K E; Duffield, T F; Edge, V L

    1998-04-01

    First DHI test milk that was sampled prior to displaced abomasum (DA) diagnosis was used to evaluate milk protein/fat ratio (PFR) for prediction of subsequent DA in dairy cows. Odds ratio, sensitivity, specificity, and likelihood ratio were determined. Twenty-seven DA cases were matched to 3 controls per case by herd and calving date. Milk was tested at a median of 19 d after calving, which was 8 d prior to the median time of DA diagnosis. Adjusted for parity and days in milk, a protein/fat ratio 0.72. Using the cut off value of 0.72, the sensitivity of PFR for DA was 80% and the specificity was 68%. A receiver operating characteristics curve indicated that the minimum sum of false negative and false positive results was at a PFR cut off value of 0.72. The likelihood ratio indicated that protein/fat ratios DHI test milk may predict subsequent DA in dairy cows.

  16. Avaliação morfológica do abomaso e ceco-cólon de bovinos Morphologic evaluation of the abomasum and cecum-colon of bovines

    Directory of Open Access Journals (Sweden)

    M.G. Cardoso

    2013-04-01

    Full Text Available A morfofisiologia relacionada à absorção de ácidos graxos voláteis (AGV ao longo do trato gastrintestinal de ruminantes não é totalmente caracterizada. Desse modo, os objetivos deste trabalho foram mensurar a extensão da superfície de absorção e determinar o índice mitótico (IM do abomaso, do ceco e da alça proximal do cólon ascendente (APCA. Dez bovinos mestiços adultos tiveram seu estômago e intestino grosso removidos imediatamente após o abate. A área total da superfície de absorção foi mensurada por meio de digitalização e análise de imagens. Cortes histológicos foram feitos para determinação do IM. A superfície absortiva do abomaso, 0,58m², foi menor (PThe morphology and physiology related to volatile fatty acid (VFA absorption throughout the gastrointestinal tract of ruminants is not totally characterized. The purpose of this work was to measure the abomasum and cecum-colon absorptive surface extension and determine the mitotic index (MI. Ten adult crossbred bovine had their stomach and large intestine removed after slaughter. The total area of the absorptive surface was measured through image capture and analysis. Histological sections were performed to measure the MI. The abomasum absorptive surface (0.58m² was lower (P<0.01 than that of the rumen (6.53m² and omasum (2.31m². The cecum-colon absorptive surface (0.23m² corresponded to 3.5% of the rumen and 10% of the omasum. The MI observed was 0.48%; 0.14%; 0.36% and 0.41% for the regions of spiral folds, pyloric, cecum and proximal loop of ascending colon, respectively. A positive correlation between abomasum tissue mass and the abomasum absorptive surface was observed. This phenomenon has also occurred in the cecum and colon. It was possible to establish regressions to facilitate measurements of the absorptive surface of the abomasum and cecum-colon.

  17. The effect of administering ketoprofen on the physiology and behavior of dairy cows following surgery to correct a left displaced abomasum.

    Science.gov (United States)

    Newby, Nathalie C; Pearl, David L; LeBlanc, Stephen J; Leslie, Ken E; von Keyserlingk, Marina A G; Duffield, Todd F

    2013-03-01

    Surgical correction of left displaced abomasum (LDA) is common in lactating dairy cattle. Despite the growing acceptance that abdominal surgery is painful, few cows are administered analgesia following LDA surgery. The objective of this research was to examine the effect of administering a label dose of ketoprofen on physiological and behavioral indicators of pain in dairy cattle. Holstein cows were enrolled in a field study following LDA surgery. Surgery was performed using the standing right flank (RF) approach or the paramedian (PARA) approach. Using a triple-blind randomized trial, each animal was assigned to receive either 3mg of ketoprofen/kg of body weight or saline (the equivalent volume) by intramuscular injection immediately following surgery and 24h postoperatively. Physiological parameters (heart rate, respiration rate, and rumen motility), blood β-hydroxybutyrate (BHBA) levels, and an assessment of cow attitude were measured on the day of surgery, and at 2 follow-up visits (visit 1=3 ± 0.9 d and visit 2=9 ± 1.2 d postsurgery; n=175). Milk production and culling were recorded for all cows enrolled in the study. Producers assessed their cows' attitudes and appetites daily for the first 3 d following surgery. A subset of cows (n=37) were fitted on the day of surgery with a 3-axis accelerometer on the hind leg to assess lying behavior. Continuous and binary outcome data were analyzed using multivariable mixed linear and mixed logistic models, respectively, with cow modeled as a random effect. Ketoprofen did not alter the physiological measures, BHBA levels, or behavioral outcomes measured. Cows subjected to RF surgery had longer lying times [model coefficient β=228.9 min; 95% confidence interval (CI): 122.2 to 335.6] in the first 3 d following surgery, and lower heart rates (β=-9.4 beats/min; 95% CI: -12 to -6.9 beats/min) at the follow-up visits, compared with animals that underwent PARA surgery. Regardless of surgical procedure, BHBA decreased from

  18. A field trial on the effect of propylene glycol on displaced abomasum, removal from herd, and reproduction in fresh cows diagnosed with subclinical ketosis.

    Science.gov (United States)

    McArt, J A A; Nydam, D V; Oetzel, G R

    2012-05-01

    The purpose was to determine the effect of oral propylene glycol (PG) administration in fresh cows diagnosed with subclinical ketosis (SCK). Measured outcomes were development of displaced abomasum (DA) and removal from herd in the first 30 d in milk (DIM), conception to first service, and time to conception within 150 DIM. Cows from 4 freestall dairy herds (2 in New York and 2 in Wisconsin) were each tested 6 times for SCK from 3 to 16 DIM on Mondays, Wednesdays, and Fridays using the Precision Xtra meter (Abbott Laboratories, Abbott Park, IL). Subclinical ketosis was defined as a blood β-hydroxybutyrate concentration of 1.2 to 2.9 mmol/L. Cows with SCK were randomized to treatment group (oral PG) or untreated control group (no PG); treatment cows were orally drenched with 300 mL of PG once daily from the day they tested 1.2 to 2.9 mmol/L until the day they tested <1.2 mmol/L. Mixed effects multivariable Poisson regression was used to assess the effect of PG on DA, removal from herd, and conception to first service; a semiparametric proportional hazards model was used to evaluate the days-to-conception outcome. A total of 741 of 1,717 (43.2%) eligible enrolled cows had at least 1 β-hydroxybutyrate test of 1.2 to 2.9 mmol/L. Of these, 372 were assigned to the PG treatment group and 369 to the control group. Thirty-nine cows (5.3%) developed a DA after testing positive for SCK and 30 cows (4.0%) died or were sold within the first 30 DIM. Based on risk ratios, control cows were 1.6 times more likely [95% confidence interval (CI)=1.3 to 2.0] to develop a DA and 2.1 times more likely (95% CI=1.2 to 3.6) to die or be sold than cows treated with PG. In addition, PG-treated cows were 1.3 times more likely (risk ratio 95% CI=1.1 to 1.5) to conceive at first insemination than control cows in 3 of the herds. No difference was observed in days to conception within 150 DIM between treatment groups (hazard ratio for PG cows=1.1, 95% CI=0.8 to 1.4), with a median time to

  19. Hematological, biochemical and ruminant parameters for diagnosis of left displacement of the abomasum in dairy cows from Southern Brazil Indicadores hematológicos, bioquímicos e ruminais no diagnóstico do deslocamento de abomaso à esquerda em vacas leiteiras do Sul do Brasil

    Directory of Open Access Journals (Sweden)

    Felipe Cardoso de Cardoso

    2008-01-01

    Full Text Available The objective of this work was to evaluate hematological, biochemical and ruminant parameters for diagnosis and treatment of the left displaced abomasum (LDA in dairy cows, in the Plateau Region of Rio Grande do Sul, Brazil. Ruminant fluid, blood and urine samples were collected from 20 cows suffering LDA and from 20 healthy cows (control. The cows with LDA showed lower values of daily milk production, body weight and corporal condition score. The use of pH reagent strips showed to be functional in the field, when compared to a digital pH meter. Ruminant dynamics was damaged in cows affected by LDA, as it was evidenced by the higher reduction time of methylene blue. Serum values of lactate, beta-hydroxybutyrate, urea, albumin, free fatty acids and cholesterol shows to be auxiliary tools in the LDA characterization.O objetivo deste trabalho foi avaliar indicadores hematológicos, bioquímicos e ruminais no diagnóstico e tratamento do deslocamento de abomaso à esquerda (DAE em vacas leiteiras, na Região do Planalto do Rio Grande do Sul, Brasil. Foram coletadas amostras de líquido ruminal, sangue e urina de 40 animais, dos quais 20 vacas com DAE e 20 vacas clinicamente sadias utilizadas como grupo controle. Os animais com DAE, quando comparados ao grupo controle, apresentaram diminuição da produção de leite diária, do peso corporal e do escore condição corporal. A utilização de fitas reagentes para medição do pH ruminal demonstrou-se eficaz em campo, em comparação ao potenciômetro digital. A dinâmica ruminal apresentou-se prejudicada nos animais com DAE, o que foi evidenciado pelos valores aumentados do tempo de redução de azul de metileno. Os valores séricos de lactato, beta-hidroxibutirato, uréia, albumina, ácidos graxos livres e colesterol apresentam-se como ferramentas auxiliares na caracterização da doença.

  20. Early IL-4 gene expression in abomasum is associated with resistance to Haemonchus contortus in hair and wool sheep breeds.

    Science.gov (United States)

    Jacobs, J R; Sommers, K N; Zajac, A M; Notter, D R; Bowdridge, S A

    2016-06-01

    Early immune events associated with reduced larval burden remain unclear in parasite-resistant breeds of sheep. Therefore, our objective was to determine breed differences in immune-related gene expression following infection with H. contortus. Gene expression in abomasal tissue and mucosa and in abomasal lymph nodes (ALN) was measured in 24 St. Croix (hair) lambs and 24 Dorset x (Finn-Rambouillet) (wool) lambs at 0 (uninfected), 3, 5 and 7 days after infection with 10 000 L3 H. contortus larvae. Expression of IL-4 in abomasal mucosa was detected on day 3 and increased to day 7 in hair lambs, but was not detectable in wool lambs. Genes that recruit neutrophils (CXCL1) and macrophages (MCP1) were upregulated in abomasal mucosa of hair lambs. Genes associated with alternative macrophage activation (ARG-1) and eosinophil activation (Gal-14) were also upregulated in the abomasal mucosa of hair lambs. Tissue remodeling genes (MMP13, PDGF) and tumour necrosis factor alpha (TNF-α) and MCP1 were upregulated in abomasal tissue of wool lambs; these lambs also had greater expression of forkhead box P3 in ALN. These data indicate a role for early IL-4 expression locally and demonstrate potential downregulation of immunity in wool sheep that could facilitate establishment of H. contortus.

  1. Responses to increasing amounts of high-oleic sunflower fatty acids infused into the abomasum of lactating dairy cows.

    Science.gov (United States)

    Drackley, J K; Overton, T R; Ortiz-Gonzalez, G; Beaulieu, A D; Barbano, D M; Lynch, J M; Perkins, E G

    2007-11-01

    Increasing the oleic acid (18:1 cis-9) content of milk fat might be desirable to meet consumer concerns about dietary healthfulness and for certain manufacturing applications. The extent to which milk fat could be enriched with oleic acid is not known. Increasing the intestinal supply of polyunsaturated fatty acids decreases dry matter intake (DMI) in cows, but the effects of oleic acid have not been quantified. In a crossover design, 4 multiparous Holstein cows were abomasally infused with increasing amounts (0, 250, 500, 750, or 1,000 g/d) of free fatty acids from high-oleic sunflower oil (HOSFA) or with carrier alone. Continuous infusions (20 to 22 h/d) were for 7 d at each amount. Infusions were homogenates of HOSFA with 240 g/d of meat solubles and 11.2 g/d of Tween 80; controls received carrier only. The HOSFA contained (by wt) 2.4% 16:0, 1.8% 18:0, 91.4% 18:1 cis-9, and 2.4% 18:2. The DMI decreased linearly (range 22.0 to 5.8 kg/d) as the infused amount of HOSFA increased. Apparent total tract digestibilities of dry matter, organic matter, neutral detergent fiber, and energy decreased as the infusion increased to 750 g/d and then increased when 1,000 g/d was infused. Digestibility of total fatty acids increased linearly as infused fatty acids increased. Yields of milk, fat, true protein, casein, and total solids decreased quadratically as infused amounts increased; decreases were greatest when 750 or 1,000 g/d of HOSFA were infused. Concentrations of fat and total solids increased at the higher amounts of HOSFA. The volume mean diameter of milk fat droplets and the diameter below which 90% of the volume of milk fat is contained both increased as HOSFA infusion increased. Concentrations of short-chain fatty acids, 12:0, 14:0, and 16:0 in milk fat decreased linearly as HOSFA increased. The concentration of 18:1 cis-9 (19.4 to 57.4% of total fatty acids) increased linearly as HOSFA infusion increased. Concentrations of 18:1 cis-9 in blood triglyceride-rich lipoproteins increased linearly as infusion increased, whereas contents of 14:0, 16:0, 18:0, total 18:1 trans, and 18:2n-6 decreased linearly. The composition and physical characteristics of milk fat can be altered markedly by an increased intestinal supply of 18:1 cis-9, which could influence processing characteristics and the healthfulness of milk fat. However, an increased supply of free 18:1 cis-9 to the intestine decreased DMI and milk production.

  2. Ultrasonographic characterization of the abomasum in sheeps with surgery-induced left displacement of abomsum%小尾寒羊皱胃左方变位动物模型皱胃超声影像学特征

    Institute of Scientific and Technical Information of China (English)

    张仁和; 杨威; 张玉明; 郭立辉; 苑学; 田宇; 张敏; 李心慰; 刘国文

    2015-01-01

    比较分析了手术致小尾寒羊皱胃左方变位动物模型造模前后皱胃B超声像图的变化情况,旨在为B超诊断反刍动物皱胃左方变位提供依据.将6只体型相近的成年雌性小尾寒羊,随机分为Ⅰ、Ⅱ组,每组3只羊,用配备5.0MHz线型探头的B超仪对2组羊皱胃进行探查,记录皱胃可探查区域及声像图特征,随后通过手术对Ⅰ组羊进行轻度皱胃左方移位,Ⅱ组羊进行重度皱胃左方移位,术后每2d对实验羊的皱胃位置进行1次B超探测,持续15d,记录各次探查结果.结果显示,手术前各实验羊均可在剑状突后腹中线及近腹中线区域探测到皱胃,声像图中皱胃壁较难分辨,但皱胃内可明显看到其内容物产生的不均一回声点或回声块,不时可看到产弱回声的皱胃皱襞;术后,2组羊在皱胃固定点附近区域都可探查到皱胃嵌于腹壁与瘤胃之间的声像图,皱胃可与周围器官明显区别开,Ⅱ组羊还可在腹腔左侧壁探查到幽门;15d的观察期内除Ⅱ组中一羊皱胃自发复位外,2组羊皱胃位置在固定点附近无太大变化,Ⅰ组羊皱胃声像图没变化,Ⅱ组羊嵌留于瘤胃与腹壁之间的皱胃厚度增加.结果表明,在B超探查中小尾寒羊皱胃声像图特征明显,在皱胃变位后能与相邻组织器官区别,B超仪具有诊断反刍动物皱胃变位疾病的潜力.

  3. 奶牛皱胃左方变位的手术治疗与术后护理%Surgical Operation Treatment and after the Technique Nurses Which the Abomasums of Cow to Change Its Position to the Left

    Institute of Scientific and Technical Information of China (English)

    周晓翠

    2006-01-01

    本文介绍了奶牛皱胃左方变位的主要临床特征、诊断特点、手术治疗的方法与效果.结合临床的观察,对皱胃变位在听诊、叩诊、直肠检查及全身症状表现等方面的特征做了比较详尽的论述.并对皱胃变位与奶牛年龄、胎次、病程长短等因素与手术效果、术后护理应注意的问题,进行了探讨.

  4. Effect of Ultrasonic Treatment on the Extracting Activity of Kid Rennet

    Institute of Scientific and Technical Information of China (English)

    ZHANG Fu-xin; LI Lin-qiang

    2005-01-01

    Using 2-3 weeks kid abomasums as materials, the ultrasound intensity, extracting time, NaC1 concentration and pH value in extracting solution and ratio of abomasums to extracting solution were studied by ultrasonic method. The results showed that the main factor affecting kid rennet activity during extraction was ultrasound intensity, and then NaC1concentration, extracting time and ratio of abomasums to extracting solution in order. Kid rennet activity reached peak with ultrasound intensity 30 W cm-2, extraction time 40 min, NaC1 concentration 8%, pH value 3.0, ratio of abomasums to extracting solution 1:15.

  5. Linear distribution of nematodes in the gastrointestinal tract of tracer lambs.

    Science.gov (United States)

    Makovcová, Katerina; Langrová, Iva; Vadlejch, Jaroslav; Jankovská, Ivana; Lytvynets, Andriy; Borkovcová, Marie

    2008-12-01

    Forty-eight tracer lambs were killed in 2004-2007. The abomasum, duodenum, small intestine (jejunum and ileum), colon and caecum were collected and processed for parasites enumeration and identification-mucosal scrapings of both abomasums and intestines were digested. Out of 48 gastrointestinal tracts examined, all were found to be positive for nematode infection. Seventeen species of gastrointestinal nematodes were recovered: Bunostomum trigonocephalum, Cooperia curticei, Haemonchus contortus, Chabertia ovina, Nematodirus battus, Nematodirus filicollis, Oesophagostomum venulosum, Teladorsagia circumcincta, Trichostrongylus axei, Trichostrongylus colubriformis, Trichostrongylus vitrinus, Strongyloides papillosus, Trichuris ovis, Trichuris globulosa, Trichuris skrjabini and Skrjabinema ovis. All species were searched for in the entire gastrointestinal tract. Six species of nematodes were recovered from abnormal sites, naturally in small numbers of lambs as well as in small amounts: Nematodirus battus in the abomasums (6.67% of lambs), N. filicollis in the caecum and in the colon (%4 and 8%, respectively), T. axei in the colon (9.52%), T. colubriformis in the colon (13.89%), T. vitrinus in the caecum (16.67%), in the colon (20.00%) and in the abomasum (3.33%). T. ovis was found in one case in the small intestine.

  6. Effect of abomasal butyrate infusion on gene expression in the duodenum of lambs

    Science.gov (United States)

    A previous study infusing butyrate into the abomasum of sheep produced increased oxygen, glucose, glutamate, and glutamine uptake by the portal-drained viscera. These changes were thought to be partially due to increases in glycolysis and cell proliferation. The purpose of this study was to evaluate...

  7. Abomasal secretion and motility in sheep : effect of diet and digesta components

    NARCIS (Netherlands)

    Bruchem, van J.

    1977-01-01

    In sheep fitted with re-entrant cannulas in the proximal duodenum, secretory rates of acid in the abomasum increased with protein or buffering content of the rations.In sheep fitted with an abomasal infusion tube and with duodenal re-entrant cannulas, proteins and inorganic buffers continuously infu

  8. Measurement of the postruminal digestibility of crude protein by the bag technique in cows.

    Science.gov (United States)

    Voigt, J; Piatkowski, B; Engelmann, H; Rudolph, E

    1985-08-01

    A new method has been developed which permits the crude protein digestibility of feedstuffs in the intestine of cattle to be measured with little effort in terms of samples and experimental work. It consists of welding 0.4 ... 0.8 g of the feedstuff (particle size: 125 ... 1000 micron) into polyamide fabric bags (25 X 40 mm) which are inserted via cannulae into the digestive tracts of fistulated cows from the abomasum/duodenum to the ileum or from the abomasum/duodenum to the faeces. The mean retention time of the bags in the animal was 8.5 +/- 2.7 h from the abomasum to the end of the ileum and (13.3 +/- 1.9 h from the abomasum to the faeces. Up to 15 bags per day and cow may be used. The random error of the method is 1.3% (absolute) when the measurements are performed on two animals using two bags each. Intestinal digestibilities of over 90% were measured for concentrate proteins (except linseed meal) and of 72 ... 95% for forage proteins. Post-ruminal digestion was virtually finished at the end of the small intestine.

  9. The interaction between Cooperia spp. and Ostertagia spp. (Nematoda: Trichostrongylidae) in cattle.

    OpenAIRE

    Frankena, K.

    1987-01-01

    In this study the presence of interaction between Cooperia oncophora and Ostertagia ostertagi, nematodes which parasitize the small intestine and the abomasum of cattle, respectively, has been investigated. Interaction is of epidemiological importance when it leads to a reduced worm burden or a lowered faecal egg output. As there were some indications that interaction between C. oncophora and O.ostertagi is immunologically, mediated experiments were carried out in which calves were given some...

  10. The toxicity of Cassia occidentalis to goats

    OpenAIRE

    Suliman, H.B.; Wasfi, I.A.; Adam, S. E. I.

    1982-01-01

    Metadata only record In 8 goats given the leaves or seeds of C. occidentalis , the main signs of poisoning were diarrhea, inappetence, dyspnoea, staggering, ataxia and recumbency. Lesions consisted of hemorrhages and congestion in the heart, lungs, abomasum and spleen, catarrhal enteritis, hepatic fatty change and necrosis, splenic haemosiderosis, pulmonary emphysema, necrosis and/or degeneration of the epithelial cells of the renal convoluted tubule, and packing of the glomeruli with endo...

  11. [The use of ultrasonography for diagnosing the cause of colic in cows. A review].

    Science.gov (United States)

    Braun, U; Nuss, K; Knubben-Schweizer, G; Gerspach, C

    2011-01-01

    Ultrasonography is a very useful technique for diagnosing the cause of colic in cows. It allows visualisation of abnormal reticular contour and occasionally of abnormal contractility in cows with reticuloperitonitis. In right-displaced abomasum, the dilated abomasum can be detected between the right abdominal wall and the liver. Fluid ingesta are seen ventrally and a gas cap of varying size dorsally. Dilated loops of small intestines that are almost always static are the main diagnostic criterion for ileus of the small intestine, but the cause of the ileus can only rarely be determined. Cholestasis can almost always be diagnosed by imaging a dilated biliary system. With obstruction at the level of the hepatic portal, only the intrahepatic biliary ducts are dilated, while a dilatation of the entire biliary tract, including the gallbladder, occurs in the case of an obstruction near the duodenal papilla. Urinary tract diseases cause colic in cows when concrement or inflammatory products become lodged in a ureter. The importance of ultrasonography in the diagnosis of diseases causing colic in cows varies. For example, with colic attributable to ileus of the small intestines, cholestasis or urinary tract disease, ultrasonography is a very useful diagnostic tool. On the other hand, for diagnosis of left or right displacement of the abomasum or caecal dilatation, ultrasonography is generally not required, but it is helpful in difficult cases to confirm or rule out a tentative diagnosis and to avoid an unnecessary exploratory laparotomy.

  12. Efficacy of fenbendazole against inhibited larvae of Ostertagia ostertagi in yearling cattle.

    Science.gov (United States)

    Williams, J C; Knox, J W; Marbury, K S; Kimball, M D; Scheide, S W; Snider, T G; David, M U

    1984-10-01

    Efficacy of fenbendazole, at doses of 7.5 and 10.0 mg/kg of body weight, against inhibited early 4th-stage larvae of Ostertagia ostertagi and other nematodes of the abomasum and intestinal tract, was investigated in naturally infected yearling heifers in late May 1982. In Louisiana, this is near the end of the period (March to May) in which maximal numbers of inhibition-prone larvae are acquired. The mean numbers of O ostertagi in 10 untreated control cattle were: adults, 4,880; developing 4th-stage larvae, 12,546; and inhibited early 4th-stage larvae, 167,931. At the 7.5 mg/kg dose level (10% liquid suspension) in 10 cattle, percentage reduction of O ostertagi in comparison with controls was: adults, 95.7%; developing 4th stages, 91.1%; and inhibited 4th stage, 55.0%. Percentage reductions of other genera were as follows: abomasum--Trichostrongylus axei, 99.6%; Haemonchus sp, 95.1%; intestinal tract--Cooperia spp, 97.8%; Trichostrongylus colubriformis, 100.0%; and Oesophagostomum radiatum 4th stage and adults, 100.0%. At the 10.0 mg/kg dose (10% liquid suspension) in 11 cattle, the percentage reduction of O ostertagi in comparison with controls was: adults, 98.6%; developing 4th stages, 92.9%; and inhibited 4th stage, 80.0%. Percentage reductions of other genera were: abomasum--T axei, 99.9%; Haemonchus sp, 98.8%; intestinal tract--Cooperia spp, 99.3%; T colubriformis, 100.0%; and Oes radiatum 4th stage and adults, 100.0%. Variability of efficacy against inhibited larvae was observed, particularly at the 7.5 mg/kg dose; at this dose, 7 of the 10 heifers in the group yielded in excess of 54,000 surviving larvae.(ABSTRACT TRUNCATED AT 250 WORDS)

  13. Bochdalek Congenital Diaphragmatic Hernia in an Adult Sheep.

    Science.gov (United States)

    Williams, R D; Katz, M G; Fargnoli, A S; Kendle, A P; Mihalko, K L; Bridges, C R

    2016-06-01

    Congenital diaphragmatic hernia (CDH) is a rare condition. The aetiology of CDH is often unclear. In our case, a hollow mass was noted on MRI. Cardiac ejection fraction was diminished (47.0%) compared to 60.5% (average of 10 other normal animals, P congenital diaphragmatic hernia (Bochdalek type) was made when the sheep underwent surgery. The hernia was right-sided and contained the abomasum. Lung biopsy demonstrated incomplete development with a low number of bronchopulmonary segments and vessels. The likely cause of this hernia was genetic malformation.

  14. Immunohistochemical localization of galanin receptors (GAL-R1, GAL-R2, and GAL-R3) on myenteric neurons from the sheep and dog stomach.

    Science.gov (United States)

    Arciszewski, Marcin Bartłomiej; Barabasz, Sylwester; Całka, Jarosław

    2008-01-01

    Galanin exerts its biological activities (inhibitory or excitatory) via three different G protein-coupled receptors. In the present study, double immunocytochemical labeling was used to localize GAL-R1, GAL-R2 and GAL-R3 on PGP 9.5-positive myenteric neurons from the dog and sheep stomach/forestomachs. In both species, the occurrence of galanin in neurons and nerve fibers of gastric ganglia was also studied. Myenteric ganglia of the dog stomach were supplied with numerous, mainly varicose, galanin-immunoreactive (IR) nerve terminals whereas the frequency of galanin-positive nerve fibers in myenteric ganglia of the ovine stomach and forestomachs was moderate. The number of PGP 9.5-IR/galanin-IR myenteric neurons was significantly lower in the dog stomach (12.3+/-1.3%) as compared to the sheep rumen (20.1+/-0.7%), omasum (19.5+/-2.9%), abomasum (23.8+/-1.2%) but not reticulum (8.1+/-0.8%). In the canine stomach the frequencies of GAL-R1, GAL-R2 and GAL-R3 expressing myenteric neurons were statistically equivalent (4.4+/-0.9%, 3.5+/-0.7% and 3.1+/-0.5%, respectively). Immunoreactivity to GAL-R1 was absent in myenteric ganglia from the ovine rumen, reticulum as well as omasum. GAL-R1 was localized on 0.5+/-0.3% of myenteric perikarya from the abomasum. GAL-R2 bearing myenteric neurons were localized in the ovine rumen (0.6+/-0.3%), reticulum (0.5+/-0.3%), omasum (1.0+/-0.2%) and abomasum (1.1+/-0.3%). The percentages of PGP 9.5-IR/GAL-R3-IR neurons were 0.8+/-0.2% in the rumen, 0.6+/-0.3% in the reticulum, 0.7+/-0.2% in the omasum and 0.9+/-0.3% in the abomasum. In all compartments of the sheep stomach, the proportions of GAL-R1, GAL-R2 and GAL-R3 expressing neurons were significantly lower when compared to analogous neuronal subpopulations present in the dog. It is suggested that, although endogenous galanin may potentially inhibit or stimulate the activity of sparse gastric enteric neurons, its general role in indirect mediation of gastric motility and/or secretion

  15. Bochdalek Congenital Diaphragmatic Hernia in an Adult Sheep

    Science.gov (United States)

    Williams, R. D.; Katz, M. G.; Fargnoli, A. S.; Kendle, A. P.; Mihalko, K. L.; Bridges, C. R.

    2016-01-01

    Summary Congenital diaphragmatic hernia (CDH) is a rare condition. The aetiology of CDH is often unclear. In our case, a hollow mass was noted on MRI. Cardiac ejection fraction was diminished (47.0%) compared to 60.5% (average of 10 other normal animals, P < 0.05). The final diagnosis of congenital diaphragmatic hernia (Bochdalek type) was made when the sheep underwent surgery. The hernia was right-sided and contained the abomasum. Lung biopsy demonstrated incomplete development with a low number of bronchopulmonary segments and vessels. The likely cause of this hernia was genetic malformation. PMID:26293994

  16. Dicty_cDB: VFH648 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 5 Bovine Abomasum cDNA Library Bos taurus cDNA 5', mRNA sequence. 44 2e-04 2 CB062515 |CB062515.1 4012285 BARC...alue N AX536940 |AX536940.1 Sequence 541 from Patent WO02064766. 50 1e-06 2 BG688...103 |BG688103.1 335557 BARC 5BOV Bos taurus cDNA 5', mRNA sequence. 44 2e-04 2 BG938001 |BG938001.1 1Abo08B0

  17. Effect of abomasal glucose infusion on splanchnic amino acid metabolism in periparturient dairy cows

    DEFF Research Database (Denmark)

    Larsen, Mogens; Kristensen, Niels Bastian

    2009-01-01

    Six Holstein cows fitted with ruminal cannulas and permanent indwelling catheters in the portal vein, hepatic vein, mesenteric vein, and an artery were used to study the effects of abomasal glucose infusion on splanchnic AA metabolism. The experimental design was a split plot, with cow as the whole...... plot, treatment as the whole-plot factor and days in milk (DIM) as the subplot factor. Cows were assigned to 1 of 2 treatments: control or infusion of 1,500 g/d of glucose into the abomasum from the day of calving to 29 DIM....

  18. Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes

    Science.gov (United States)

    2010-01-01

    Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and was ranked as the most stable in abomasum and lymph node tissues. On the other hand, SDHA was the most stable in the small intestine followed by RPLO and GAPDH. These findings demonstrate the importance of correctly choosing reference genes prior to relative quantification. In addition, we determined that reference genes used in sheep from temperate regions, when properly tested, can be applied in animals from tropical regions such as the Brazilian Somalis sheep. PMID:21637421

  19. Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes

    Directory of Open Access Journals (Sweden)

    Lilian Giotto Zaros

    2010-01-01

    Full Text Available Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO, glyceraldehyde 3-phosphate dehydrogenase (GAPDH and succinate dehydrogenase complex subunit A (SDHA, were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and was ranked as the most stable in abomasum and lymph node tissues. On the other hand, SDHA was the most stable in the small intestine followed by RPLO and GAPDH. These findings demonstrate the importance of correctly choosing reference genes prior to relative quantification. In addition, we determined that reference genes used in sheep from temperate regions, when properly tested, can be applied in animals from tropical regions such as the Brazilian Somalis sheep.

  20. Spontaneous poisoning by Ricinus communis (Euphorbiaceae in cattle

    Directory of Open Access Journals (Sweden)

    Samuel S.C. Albuquerque

    2014-09-01

    Full Text Available The aim of this study is to report cases of spontaneous poisoning of cattle by Ricinus communis (castor beans in Paraíba, a semiarid region of northeastern Brazil. The cases were observed in 2 herds on neighboring properties in 2013. Clinical signs developed within 6-24 h and consisted of weakness, tachycardia, dyspnea, profuse watery diarrhea, dehydration, depression, instability, cramps, permanent lateral recumbency and death within 48-72 h. Of the 60 cattle at risk, 19 were affected and 14 died. Five fully recovered after the course of 12 days. Three animals were necropsied. The main gross lesions were hemopericardium, hemothorax, pulmonary edema, petechial hemorrhages in the epicardium and endocardium, ecchymoses at the papillary muscles and suffusions on the intercostal muscles. Hemorrhages were also observed in the abdominal cavity, spleen and mucosa of the abomasum and small intestine. The rumen content was liquid with a large amount of castor bean seeds. There were circular, whitish and focally diffuse areas in the liver parenchyma. The main microscopic lesions consisted of multifocal coagulative myocardial necrosis with the presence of mononuclear cell infiltration and varying degrees of bleeding between cardiac muscle fibers. The abomasum and small intestine mucosae and submucosa had mild edema and mononuclear and polymorphonuclear inflammatory cell infiltration. The diagnosis of R. communis was based on the history of plant consumption, clinical signs, pathology of the disease and the presence of large amounts of castor bean seeds in the forestomachs.

  1. Source of dietary protein influences kinetics of plasma gut regulatory peptide concentration in response to feeding in preruminant calves.

    Science.gov (United States)

    le Huërou-Luron, I; Gestin, M; Le Dréan, G; Romé, V; Bernard, C; Chayvialle, J A; Guilloteau, P

    1998-03-01

    The kinetics of the peripheral plasma concentrations of eight gut regulatory peptides were examined in response to feeding in preruminant calves. Two experiments were carried out in animals fed milk substitutes either based on milk protein (control diet) or in which casein had been replaced by hydrolyzed fish (fish diet in experiment 1) or whey (whey diet in experiment 2) protein concentrate. In contrast to the control diet, the latter two did not coagulate within the abomasum. No variation was observed in plasma concentrations of gut regulatory peptides during 1-1.4 hr before the morning meal regardless of the nature of the dietary protein. With the control diet, the meal was followed by an increase in cholecystokinin, gastrin and gastric inhibitory polypeptide and a fall in secretin, vasoactive intestinal polypeptide and motilin, whereas no significant change was observed for somatostatin and pancreatic polypeptide. The replacement of casein by protein substitutes did not greatly modify the pattern of plasma responses to feeding, but the prefeeding and postfeeding levels were highly affected. We conclude that the most important characteristic influencing plasma gut peptide concentrations is the ability of dietary protein to clot in the abomasum, consequently determining the pattern of gastric emptying, and that variations appear depending on the origin of protein substitutes in relation to the duodenal content and mainly to the digesta pH.

  2. Experimental induction of abdominal tympany, abomasitis, and abomasal ulceration by intraruminal inoculation of Clostridium perfringens type A in neonatal calves.

    Science.gov (United States)

    Roeder, B L; Chengappa, M M; Nagaraja, T G; Avery, T B; Kennedy, G A

    1988-02-01

    The etiologic role of Clostridum perfringens type A in the acute abdominal syndrome characterized by abomasal and rumen tympany, abomasitis, and abomasal ulceration was investigated in neonatal calves. Eight calves, 4 to 12 days old, were inoculated intraruminally with toxigenic C perfringens type A. Before and after C perfringens inoculation, blood samples were collected from all calves for blood gas and serum biochemical analysis and for determination of serum copper concentration; ruminal fluid was obtained for isolation of C perfringens. Calves were monitored daily for clinical signs of the syndrome and, depending on the severity of clinical signs, they were either euthanatized or redosed within 4 to 7 days. After necropsy, specimens obtained from the abomasum and rumen for macroscopic and microscopic examination and for anaerobic bacteriologic culture were processed in routine manner. Intraruminal inoculation of C perfringens type A into healthy calves induced anorexia, depression, bloat, diarrhea, and in some calves, death. Serum copper concentration was within normal range. Necropsy revealed variable degrees of abomasitis, petechial and ecchymotic hemorrhages, and ulcers (ranging from pinpoint to nearly perforate) in the abomasum. Seven of those calves also had multiple trichobezoars in the rumen. These necropsy findings were not seen in calves (controls) given distilled H2O only. In affected calves, acute abdominal syndrome was unrelated to copper deficiency, and C perfringens type A given intraruminally was able to induce clinical signs similar to those of the naturally acquired disease.

  3. [The surgical treatment of left-sided abomasal displacement in the cow: a literature review].

    Science.gov (United States)

    Steiner, A

    1996-01-01

    In this paper, a literature review of the 4 most common surgical techniques for treatment of left displacement of the abomasum in cattle is provided, and advantages and disadvantages of the different techniques are described. Many aspects as economy, cosmetics, stage of reproduction, degree of periparturient abdominal edema, availability of assistants, and the surgeons preference determine the decision making process of the technique to be chosen. It is the author's recommendation to perform the left flank abomasopexy in cattle during the last months of pregnancy, the percutaneous toggle-pin fixation as economic procedure in less valuable animals to afford savings to the owner, and the right flank omentopexy in all other cases. Becoming acquainted with the right flank omentopexy has the advantage that this is the technique of choice for correction of right displacement of the abomasum. Ventral paramedian abomasopexy has the distinct disadvantage that 2 or 3 assistants are required to cast and position the animal. Ventral paramedian abomasopexy is, therefore, only recommended in cases, where the cosmetic result plays an important role in the decision making process.

  4. Cryopreservation of roe deer abomasal nematodes for morphological identification.

    Science.gov (United States)

    Beraldo, Paola; Pascotto, Ernesto

    2014-02-01

    Conventional methods to preserve adult nematodes for taxonomic purposes involve the use of fixative or clearing solutions (alcohol, formaldehyde, AFA and lactophenol), which cause morphological alterations and are toxic. The aim of this study is to propose an alternative method based on glycerol-cryopreservation of nematodes for their subsequent identification. Adults of trichostrongylid nematodes from the abomasum of roe deer (Capreolus capreolus Linnaeus) were glycerol-cryopreserved and compared with those fixed in formaldehyde, fresh and frozen without cryoprotectans. Morphology, transparency and elasticity of the anterior and posterior portion of male nematodes were compared, especially the caudal cuticular bursa and genital accessories. The method presented is quick and easy to use, and the quality of nematode specimens is better than that of nematodes fixed by previously used fixatives. Moreover, glycerol cryopreserved nematodes can be stored for a long time at -20 degrees C in perfect condition and they could be suitable for further analyses, such as histological or ultrastructural examinations.

  5. Effect of abomasal glucose infusion on plasma concentrations of gut peptides in periparturient dairy cows

    DEFF Research Database (Denmark)

    Larsen, Mogens; Relling, A E; Reynolds, C K;

    2010-01-01

    plasma concentrations of ghrelin were greatest prepartum and lowest at 4 d postpartum, giving a quadratic pattern of change over the transition period. Positive portal venous-arterial and hepatic venous-arterial concentration differences were observed for glucagon-like peptide 1(7-36) amide. A negative......Six Holstein cows fitted with ruminal cannulas and permanent indwelling catheters in the portal vein, hepatic vein, mesenteric vein, and an artery were used to study the effects of abomasal glucose infusion on splanchnic plasma concentrations of gut peptides. The experimental design...... was a randomized block design with repeated measurements. Cows were assigned to one of 2 treatments: control or infusion of 1,500 g of glucose/d into the abomasum from the day of parturition to 29 d in milk. Cows were sampled 12 ± 6 d prepartum and at 4, 15, and 29 d in milk. Concentrations of glucose...

  6. Effect of polyethylene glycol on rumen volume and retention time of liquid and particulate matter along the digestive tract in goats fed tannin-rich carob leaves (Ceratonia siliqua).

    Science.gov (United States)

    Silanikove, N; Gilboa, N; Nitsan, Z

    2001-04-01

    The present work studied the effects of tannins in carob leaves (CL) on rumen volume and kinetics, and on the retention time of fluid and particulate components of the digesta along the gastrointestinal tract (GIT) in goats. The experimental design was a two factor crossover experiment, i.e. in phase 1, two goats were fed CL and 2 CL and polyethylene glycol (PEG) and in phase 2, the treatments were switched. The main effects of tannins were depression of the rumen fluid and particulate content of the rumen, acceleration of the passage of liquid from the abomasum, and delay of the passage of digesta in the intestine. The overall effect was a delay in the passage of fluid and particulate matter throughout the entire GIT. It is hypothesised that these responses are largely the consequence of the interaction of tannins with digestive enzymes and the epithelium lining of the digestive tract.

  7. Decontamination effectiveness of bentonite in pigs and sheep repeatedly contaminated with radiocesium

    Energy Technology Data Exchange (ETDEWEB)

    Kossakowski, S.; Dziura, A.; Zuk, M.M. [National Veterinary Research Institute, Pulawy (Poland)

    1995-12-31

    The investigations were conducted on 10 pigs and 10 sheep contaminated daily for 21 days with {sup 137}CsCl and fed twice a day a normal diet or the same diet supplemented with 10% of bentonite. The animals were killed at days 7, 14 and 21 of radiocesium administration and after cessation of the administration. The abomasum, liver, kidneys, lung, spleen, brain, heart, muscles, tongue and skin were sampled for radiometric determinations. The animals fed a bentonite-supplemented diet revealed substantially lower contents of Cs-137 as compared to the controls. The radioactivities of pig and sheep organs after 21 d Cs-137 administration were lower by about 67.5 and 81.3%, respectively, compared to the controls. The cessation of Cs-137 administration decreased organ radioactivities in bentonite-fed sheep and pigs by about 74.5 and 64.1%, respectively, compared to those in animals without bentonite. (author). 12 refs, 2 tabs.

  8. A recombinant DNA vaccine encoding C. andersoni oocyst wall protein induces immunity against experimental C. parvum infection.

    Science.gov (United States)

    Zheng, Jun; Ren, Wenzhi; Pan, Qingshan; Wang, Qiuyue; Elhag, I A Elfaki; Li, Jianhua; Li, Mingying; Gong, Pengtao; Liu, Yingli; Zhang, Xichen

    2011-06-30

    Cryptosporidium andersoni parasited in the abomasum has been demonstrated as a cause of reduction of milk production in dairy cow. In this study, a novel chimeric DNA vaccine pVAX1-AB was constructed and the efficacy against Cryptosporidium parvum was determined. BALB/c mice were divided into 3 groups and immunized with DNA vaccine expressing the oocyst wall protein, AB protein of C. andersoni, the recombinant plasmid containing the AB gene, respectively. After inoculation of 1 × 10(6) oocysts of C. parvum, the humoral and cellular immune responses were detected. Experimental results showed that the recombinant plasmid can induce corresponding specific antibody response, simultaneously influenced cellular immune responses, and provided greater protection rate (48.6%) than the other groups. These results indicated that chimeric DNA vaccine has a potential in Cryptosporidium vaccine development.

  9. Transport and recycling of radiocesium in the alimentary tract of reindeer

    Directory of Open Access Journals (Sweden)

    Hans Staaland

    1990-09-01

    Full Text Available Transport of radiocesium between the body pools and the alimentary tract was studied in 7 reindeer calves. Comparisons were made between reindeer receiving the Cs- binder Prussian blue (Ammon-ium-ironhexacyanoferrate and untreated animals. The calves were fed lichens contaminated with 134Cs + 137Cs from the Chernobyl accident (about 10 kBq/day for 4 weeks. Absorption and secretion of radiocesium, Na and K in the alimentary tract were calculated using 51Cr-EDTA as a reference substance. Thirteen sections of the alimentary tract were sampled and analysed for radionuclides and chemical composition. In 4 animals, feeding with contaminated lichens continued until they were slaughtered, whereas in the 3 others the lichen feeding terminated 4 days before slaughter. The activity concentration of Cs nuclides increased 5-17 - fold from duodenum to the distal colon, whereas the concentration of Na decreased and K remained almost constant. Radiocesium, Na and K were secreted into the rumen, the omasum and the abomasum, whereas Na and K also were secreted into the proximal small intestine. Prussian blue had no effect on Na and K recycling, but the flow of radiocesium from the abomasum to the anus and the fecal excretion increased markedly. In the 3 animals where feeding with contaminated lichens was disconutinued 4 days before slaughter, endogenous Cs was continuously recycled between the body pools and the alimentary tract. The net exchange of radiocesium between body pools and the alimentary tract was more than 4 times the amount ingested when lichens were fed. It is concluded that radiocesium is rapidly recycled between the alimentary tract and the other body pools. Cs-binders like ammonium-ironhexacyanoferrate may bind both endogenous Cs and Cs from feed.

  10. An epidemiologic study of disease in 32 registered Holstein dairy herds in British Columbia.

    Science.gov (United States)

    van Dorp, R T; Martin, S W; Shoukri, M M; Noordhuizen, J P; Dekkers, J C

    1999-07-01

    Data recorded in a herd health management system were obtained from 32 registered Holstein dairy herds from British Columbia. Frequencies of disease were described, and the effect of herd, age, year, season, and the interrelationships between diseases within a lactation on the occurrence of disease were evaluated. Lactational incidence rates were computed for diseases with a short period of risk (ie, udder edema, milk fever, retained placenta, metritis, displaced abomasum, and ketosis), whereas for diseases with a longer period of risk (ie, cystic ovaries, mastitis and stable footrot), incidence densities were calculated. Overall, the disease incidence was low and showed an increase in frequency by year, which we attributed to more observing and complete recording by the owner, rather than an actual increase in disease incidence. Most diseases occurred early in lactation and their frequency increased with lactation number; the exception was udder edema, which occurred mainly during the first 2 lactations. An informal path model of disease interrelationships was made conditional on herd. Based on the results we inferred 2 independent pathways: one started by udder edema, and the other by milk fever. Udder edema was directly associated with mastitis occurrence from 0 to 30 d in lactation, metritis, and cystic ovaries. Mastitis from 0-30 d in lactation increased the risk of both mastitis from 31-150 d in lactation and cystic ovaries. Both of these increased the risk of late lactation mastitis. Milk fever was directly related with displaced abomasum, which increased the risk of footrot. In general, diseases that occurred in early lactation tended to increase the risk of other diseases later in lactation.

  11. Digestibility of Duddingtonia flagrans chlamydospores in ruminants: in vitro and in vivo studies

    Directory of Open Access Journals (Sweden)

    Mendoza-de-Gives Pedro

    2009-12-01

    Full Text Available Abstract Background The use of Duddingtonia flagrans as a tool for the biological control of gastrointestinal nematodes (GIN is a promising alternative to anthelmintics. The chlamydospores of D. flagrans are orally dosed and their thick cell wall gives them the capacity to resist digestion and pass through the gastrointestinal tract (GIT. Chlamydospores reaching the faeces are able to germinate and trap nematode larvae. The efficacy of this control method is based on reducing the numbers of infective larvae leaving the faeces. Techniques have recently been developed for quantifying the numbers of chlamydospores in faeces. As the number of non-digested spores could be relevant in the design and optimization of dosing programmes for the control of GIN infective larvae, the aim of the present study was to estimate the loss of D. flagrans chlamydospores during their passage through the ruminant gastrointestinal tract using in vitro and in vivo techniques. Results After in vitro rumen digestion, chlamydospore recovery was not different from the quantity originally incubated (undigested spores (P > 0.05. In vitro rumen+abomasum digestion caused nearly 36% loss of the chlamydospores originally incubated (P In vivo apparent chlamydospore digestibility in sheep showed a loss of 89.7% of the chlamydospores (P Conclusions The loss of chlamydospores was evident under in vitro and in vivo conditions. Negligible amounts of spores were lost during the in vitro rumen digestion. However, in vitro rumen+abomasum digestion resulted in a chlamydospore loss of approximately 36%. In vivo passage through the sheep GIT resulted in a total loss of 89.7% of the orally administered spores.

  12. Eficiência microbiana e parâmetros ruminais em bovinos alimentados com dietas à base de volumosos tropicais Microbial efficiency and ruminal parameters in cattle fed diets based on tropical forage

    Directory of Open Access Journals (Sweden)

    Luciano da Silva Cabral

    2008-05-01

    Full Text Available Objetivou-se com este trabalho determinar o fluxo de compostos nitrogenados no abomaso, a degradação ruminal dos carboidratos totais e da matéria orgânica, a eficiência microbiana, a concentração de N-NH3 e o pH ruminal em bovinos alimentados com dietas à base de silagem de milho, silagem de capim-elefante ou feno de capim-tifton 85. Foram utilizados seis bovinos fistulados no rúmen e abomaso em um delineamento quadrado latino 3 × 3 duplo. Os fluxos de matéria seca da digesta e da matéria seca microbiana no abomaso foram determinados a partir da utilização de fibra em detergente neutro indigestível e das bases purinas como indicador microbiano, respectivamente. As concentrações de NH3 e o pH ruminal foram determinados antes e 2, 4 e 8 horas após a alimentação. As dietas à base de silagem de milho e de feno de capim-tifton 85 proporcionaram os maiores fluxos de compostos nitrogenados no abomaso. As bactérias isoladas do rúmen apresentaram em média 8,89% de N total e 18,40 para a relação N-RNA:N-total. A dieta à base de silagem de milho promoveu maior degradação ruminal da matéria orgânica (2,96 kg/dia e dos carboidratos totais (3,07 kg/dia e maior fluxo de massa microbiana para o abomaso (788,28 g/dia. As dietas à base de feno de capim-tifton 85 e de silagem de capim-elefante resultaram em maior eficiência microbiana, cujos valores foram 28,10 e 30,39 g de N microbiano/kg de carboidratos degradados no rúmen. As menores concentrações de NH3 e pH ruminal, considerando o tempo após a alimentação, ocorreram quando fornecida a dieta à base de silagem de milho, o que possivelmente afetou negativamente a eficiência microbiana.The objectives of the present work were to determine nitrogen compounds flow into the abomasum, total carbohydrate and organic matter ruminal degradation, microbial efficiency, N-NH3 concentration and ruminal pH in cattle fed with diets based on corn silage, elephant grass silage and Tifton

  13. 7~35日龄羔羊胃肠胃蛋白酶、凝乳酶、乳糖酶活性与其 mRNA 相对表达量和胃肠发育的关系研究%A Study on Relationships between the Activities of Repsin,Chymosin, Lactase and Their mRNA Relative Expressional Volumes and Development of the Stomach and Intestines of 7-35 Day Old Lambs

    Institute of Scientific and Technical Information of China (English)

    牛越峰; 雒秋江; 陈勇; 李凤鸣; 张慧玲

    2014-01-01

    powder substitute (MPS)(n =28)or fish meal substitute (FMS)(n =28)respectively,and from 21 day old,8 of lamb fed with MPS or FMS were turned to be fed with starter ration respectively. Each group of lamb at 7,14,21,28 and 35 day old was sacrificed to take the abomasum and duodenum tis-sue,measuring the activities of the pepsin,chymosin and lactase and the corresponding relative expression of mRNA,for studying the development of 3 digestive enzymes of lamb fed with MPS,FMS and starter ra-tion respectively.The result showed that in abomasum or duodenum tissue of newborn lamb the activities of the chymosin and lactase were decreased with the age of lamb by 1.3 U/g (r 2 = 0.92,P 0.05)respectively,but the pepsin activity was weakly related with the age.The relationships be-tween the activities of the pepsin,chymosin and lactase and thier corresponding relative expression of mR-NA was poor,the mRNA expressions of the pepsin and chymosin were fluctuated by high-low-high with the age,however the activity of lactose was not significantly changed with the age of lamb.When FMS was fed,the activities of pepsin,and chymosinwere decreased,the weight of abomasum was (36.0 ±3.0)g at 7 day old of lamb,and was (38.3±5.4)g at 35 day old,suggesting a growth retardation.Turning to starter ration at 21 days of lamb fed priviously with MPS did not significantly affect the activities of the pepsin, chymosin and lactase.But the abomasum and duodenum and daily weight gains of the lambs reduced.Fur-thermore,the activities of pepsin,chymosin and lactase in the milk substitutes,which were fed priviously to the lambs,tended to declining which affected obviously abomasum and duodenum and daily weight gains of the lambs.It is concluded that the pepsin activity is weakly related with the age of lamb,but the activities of chymosin and lactase were decreased with the age;the milk substitutes may affect the latter digestion and growth of lamb;the digestive obstruction on FMS of lamb may mainly be because

  14. Effect of pelleting on efficacy of sericea lespedeza hay as a natural dewormer in goats.

    Science.gov (United States)

    Terrill, T H; Mosjidis, J A; Moore, D A; Shaik, S A; Miller, J E; Burke, J M; Muir, J P; Wolfe, R

    2007-05-15

    Resistance of gastrointestinal nematodes (GIN) to anthelmintic treatment has increased pressure to find alternative, non-chemical control methods. Feeding hay of the high condensed tannin (CT) forage sericea lespedeza [SL; Lespedeza cuneata (Dum-Cours.) G. Don.] to sheep and goats has reduced GIN fecal egg count (FEC) and worm numbers in the abomasum and small intestines. This effect has been reported with both unground (long) and ground hay. Pelleting of ground hay increases ease of storage, transport, and feeding, but heating during the pelleting process could reduce biological activity of CT. Eighteen naturally GIN-infected 5-6-month-old Kiko-Spanish cross bucks were fed pelleted and ground SL hay and ground bermudagrass [BG; Cynodon dactyon (L.) Pers.] hay diets (n=6 per treatment) in a confinement trial. The bucks were fed the ground BG hay (75% of daily intake) plus a pelleted 16% CP commercial goat chow (25% of daily intake) for 3 weeks, after which they were assigned to treatment groups based upon FEC, 12 animals were switched to ground and pelleted SL hay plus goat chow for 4 weeks, and then all animals were fed the BG ration for one additional week. Throughout the trial, feces and blood were collected from individual animals weekly to determine FEC and blood packed cell volume (PCV), respectively. All goats were slaughtered at the end of the trial, with adult worms in the abomasum and small intestines recovered, counted, and identified to species. Both forms of SL hay reduced (P<0.05) FEC in goats relative to BG hay-fed animals, with a greater reduction in goats fed the SL pellets. There was no effect on PCV until the final sampling date, when the SL pellet-fed goats' PCV increased (P<0.05) compared with the other treatments. Feeding pelleted SL reduced (P<0.05) abomasal worms, primarily Haemonchus contortus, relative to the BG hay-fed goats. Worm numbers in the goats fed ground SL hay were intermediate. Pelleting SL hay enhanced its efficacy against

  15. Pathomorphological and microbiological studies in sheep with special emphasis on gastrointestinal tract disorders

    Directory of Open Access Journals (Sweden)

    Sarvan Kumar

    2015-08-01

    Full Text Available Aim: The present study was envisaged to elucidate the pathomorphological and microbiological aspects of gastrointestinal tract (GIT disorders of sheep/lambs. Materials and Methods: Samples for research were collected from 12 sheep died with a history of GIT disorders which were brought for post-mortem examination to the Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, for pathomorphological and microbiological examination. Results: Gross pathological changes in various organs noticed were abomasitis, congestion and hemorrhages in intestine; necrotic foci on liver surface; enlarged, hard, and indurated mesenteric lymph nodes, hydropericardium, congestion, hemorrhages and consolidation of lungs and congestion and soft kidneys as the major change. On histopathological examination, there were abomasitis with leukocyte infiltration, enteritis with desquamation of mucosal epithelium and goblet cell hyperplasia, lymphadenitis with depletion of lymphocytes in the germinal center of lymphoid follicle, and splenitis with depletion of lymphocytes in the white pulp. In the liver congestion, degenerative changes in hepatocytes including cloudy swelling, fatty changes, congestion in sinusoids, and dilatation of sinusoids leading to atrophy of hepatocytes. Lungs evidenced edema, congestion, emphysema, serous inflammation, thickening of interlobular septa, fibrinous pleuritis, and peribronchiolar lymphoid follicle formation. Heart revealed sarcocystosis, fibrinous pericarditis, and hyalinization of the myocardium. In kidneys, congestion, focal interstitial nephritis, hyaline degeneration, and coagulative necrosis were seen. For microbiological aspects; cultural isolation was done from samples of liver, abomasum, mesenteric lymph nodes, spleen, heart blood, lungs, and kidneys from the carcasses of sheep/lambs. Escherichia coli was the only bacterium isolated during present studies. E. coli isolates from

  16. Produção Microbiana e Parâmetros Ruminais de Novilhos Alimentados com Dietas Contendo Vários Níveis de Concentrado Microbial Production and Ruminal Parameters in Bullos Fed Diets Containing Different Concentrate Levels

    Directory of Open Access Journals (Sweden)

    Luís Carlos Vinhas Ítavo

    2002-06-01

    Full Text Available Objetivou-se estudar a eficiência de síntese microbiana e parâmetros ruminais em bovinos. Foram utilizados cinco bovinos da raça Nelore, não-castrados, com 165 kg, fistulados no rúmen, abomaso e íleo. O delineamento foi em blocos casualizados com cinco períodos de coleta e quatro tratamentos. Os tratamentos consistiram de quatro níveis de concentrado na dieta (20, 40, 60 e 80%. O volumoso utilizado foi feno de capim-Tifton 85 (Cynodon dactylon (L. Pers.. As dietas foram isonitrogenadas com 15% de proteína bruta. O indicador microbiano utilizado foi as bases purinas. As análises estatísticas do pH ruminal e das concentrações de N-NH3 foram realizadas em esquema de parcelas subdivididas, tendo na parcela os tratamentos e na subparcela o tempo de coleta. As médias para compostos nitrogenados totais presentes no abomaso e N microbiano foram 68,58 e 60,75 g/dia, respectivamente. A quantidade de carboidratos totais degradados no rúmen (CHODR foi 1,37 kg/dia. Foi observado para a eficiência microbiana valor de 392,4 g MS microbiana/kg CHODR. A composição de bactérias e a eficiência de síntese microbiana não foram influenciadas pelo nível de concentrado das dietas.It was aimed to study the efficiency of microbial synthesis and ruminal parameters in bovine. It were used five 165 kg growing no-castrated Nellore bulls, rumen, abomasum and ileum fistulated.A randomized blocks design with four treatments and five collection periods was used. The treatments consisted of four concentrate levels (20, 40, 60 and 80%. The roughage used was Tifton 85' (Cynodon dactylon (L. Pers. Bermudagrass hay. The diets were isonitrogen with 15% of crude protein. The microbial marker used was purine basis. The averages for total compound nitrogen in the abomasum and microbial nitrogen were 68.58 and 60.75 g/day, respectively. The amount total rumen carbohydrates degraded (CHODR was 1.37 kg/day. It was observed for microbial efficiency 392.4 g DM

  17. The effect of large milk meals on digestive physiology and behaviour in dairy calves.

    Science.gov (United States)

    Ellingsen, Kristian; Mejdell, Cecilie M; Ottesen, Nina; Larsen, Stig; Grøndahl, Ann Margaret

    2016-02-01

    It is commonly believed that young calves should not be fed more than about 2l of milk per meal. If calves are fed beyond this volume, it is said that the capacity of the abomasum may be exceeded and that milk could enter the rumen. This can disturb the microbial flora/fauna of the rumen and increase the risk of indigestion, diarrhoea and reduced growth. The aim of this study was to examine the effect of large milk meals on digestive physiology and behaviour in dairy calves. Six calves (19-23days of age at the beginning of the experiment) were fed 2l of warm whole milk by teat bottle three times per day, which was the recommended Norwegian feeding regime at the time. The calves were given free access to hay, concentrates and water. During three morning feeding sessions, each separated by 48h, all calves were offered larger meals. The offered amounts were calculated according to the within patient 3-level Response Surface Pathway (RSP) design. The milk given on the three test days contained a contrast medium (barium sulphate), and the animals were radiographed before, during and immediately after intake to reveal whether milk entered the rumen. Four out of the six calves drank more than 5l in one meal and the highest voluntary intake was 6.8l in one meal (13.2% of BW). Abdominal radiographs showed that the abomasum has a large ability for distension. Milk in the rumen was not observed in any of the calves, regardless of intake. The behaviour of the calves was observed for 2h after each test session. No behaviour indicating abdominal pain or discomfort was observed regardless of intake. The results indicate that when warm whole milk is administered from a teat bottle, farmers can increase the amount of milk they offer their calves beyond the traditionally recommended portion size without risk of milk entering the rumen. Hence, farmers who want to feed their calves more milk can do so by increasing meal sizes, and not necessarily by introducing an additional meal.

  18. Lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep.

    Science.gov (United States)

    Min, B R; Attwood, G T; Reilly, K; Sun, W; Peters, J S; Barry, T N; McNabb, W C

    2002-10-01

    Condensed tannins in forage legumes improve the nutrition of sheep by reducing ruminal degradation of plant protein and increasing crude protein flow to the intestine. However, the effects of condensed tannins in forage legumes on rumen bacterial populations in vivo are poorly understood. The aim of this study was to investigate the specific effects of condensed tannins from Lotus corniculatus on four proteolytic rumen bacteria in sheep during and after transition from a ryegrass (Lolium perenne)-white clover (Trifolium repens) diet (i.e., low condensed tannins) to a Lotus corniculatus diet (i.e., higher condensed tannins). The bacterial populations were quantified using a competitive polymerase chain reaction. Lotus corniculatus was fed with or without ruminal infusions of polyethylene glycol (PEG), which binds to and inactivates condensed tannins, enabling the effect of condensed tannins on bacterial populations to be examined. When sheep fed on ryegrass-white clover, populations of Clostridium proteoclasticum B316T, Butyrivibrio fibrisolvens C211a, Eubacterium sp. C12b, and Streptococcus bovis B315 were 1.5 x 10(8), 1.1 x 10(6), 4.6 x 10(8), and 7.1 x 10(6) mL(-1), respectively. When the diet was changed to Lotus corniculatus, the average populations (after 8-120 h) of C. proteoclasticum, B. fibrisolvens, Eubacterium sp., and S. bovis decreased (P Lotus corniculatus, the populations of C. proteoclasticum, B. fibrisolvens, Eubacterium sp., and S. bovis were higher (P Lotus corniculatus without the PEG infusion, with average populations (after 8-120 h) of 4.9 x 10(7), 3.8 x 10(5), 1.9 x 10(8), and 1.0 x 10(6), respectively. Sheep fed the Lotus corniculatus diet had lower rumen proteinase activity, ammonia, and soluble nitrogen (P Lotus corniculatus plus PEG. The Lotus corniculatus diet reduced rumen nitrogen digestibility (P Lotus corniculatus and in sheep fed Lotus corniculatus plus PEG, but nonmicrobial non-ammonia nitrogen flux to the abomasum was higher (P

  19. The effect of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed on Lotus corniculatus L.

    Science.gov (United States)

    Waghorn, G C; Ulyatt, M J; John, A; Fisher, M T

    1987-01-01

    Sheep were used to evaluate the nutritional consequences of a low condensed-tannin concentration (22 g/kg dry matter (DM)) in lotus (Lotus corniculatus L.) (control group) compared with lotus given to sheep receiving intraruminal polyethylene glycol (PEG) infusion (PEG group). PEG selectively binds to tannins and prevents tannins from binding proteins. DM intakes (1430 (SE 28) g/d) and digestibility of energy (663 (SE 4.5) kJ/MJ intake) were similar for both groups but the apparent digestion of nitrogen was lower in the control sheep (0.70) than in the PEG sheep (0.78; P less than 0.001). The proportion of N apparently digested before the abomasum (i.e. in the rumen) was lower (P less than 0.05) in control sheep (0.12) than in PEG sheep (0.21; P less than 0.05). Rumen ammonia concentrations were lower (P less than 0.001) in control sheep than in PEG sheep. The proportion of neutral-detergent fibre (NDF) digested in the rumen was similar for both groups (0.48 (SE 0.012)) but less energy was digested in the rumen of the control (0.42) than of the PEG sheep (0.47; P less than 0.05). The flux of essential amino acids (EAA) through the abomasum of control sheep was 50% greater than that in PEG sheep; flux of non-essential amino acids (NEAA) was 14% higher in control than in PEG sheep. Apparent digestibility of EAA in the small intestine was similar for both treatments (0.67), but NEAA were less well digested in the control (0.55) than in the PEG sheep (0.69). The presence of tannins in the control group increased net apparent absorption of threonine (57%), valine (89%), isoleucine (94%), leucine (30%), tyrosine (41%), phenylalanine (93%), histidine (90%) and lysine (59%), and reduced NEAA absorption by 10%, compared with PEG sheep.

  20. Accumulation of monepantel and its sulphone derivative in tissues of nematode location in sheep: pharmacokinetic support to its excellent nematodicidal activity.

    Science.gov (United States)

    Lifschitz, A; Ballent, M; Virkel, G; Sallovitz, J; Viviani, P; Lanusse, C

    2014-06-16

    compared to MNP. The parent drug and its sulphone metabolite were detected in the different segments of the sheep intestine. MNPSO₂ concentrations in the different intestine sections sampled were significantly higher compared to those measured in the abomasum. Although MNP is metabolized to MNPSO₂ in the liver, the large concentrations of both anthelmintically active molecules recovered during the first 48 h post-treatment from the abomasum and small intestine may greatly contribute to the well-established pharmacological activity of MNP against GI nematodes.

  1. Presence of Selected Methanogens, Fibrolytic Bacteria, and Proteobacteria in the Gastrointestinal Tract of Neonatal Dairy Calves from Birth to 72 Hours.

    Directory of Open Access Journals (Sweden)

    Cesar E Guzman

    Full Text Available The microbial communities in the gastrointestinal tract of a young calf are essential for the anatomical and physiological development that permits a transition from milk to solid feed. Selected methanogens, fibrolytic bacteria, and proteobacteria were quantified in the rumen fluid and tissue, abomasum fluid, cecum fluid and tissue, and feces of Holstein bull calves on day 0 (0-20 mins after birth, day 1 (24 ± 1 h after birth, day 2 (48 ± 1 h after birth, and day 3 (72 ± 1 h after birth. Methanogens, fibrolytic bacteria, and Geobacter spp. were found to be already present from birth, indicating that microbial colonization of the gastrointestinal tract occurred before or during delivery. The abundance of methanogens and Geobacter spp. differed between the days tested and between compartments of the digestive tract and feces, but such difference was not observed for fibrolytic bacteria. Our findings suggests that methanogens might have an alternative hydrogen provider such as Geobacter spp. during these early stages of postnatal development. In addition, fibrolytic bacteria were present in the rumen well before the availability of fibrous substrates, suggesting that they might use nutrients other than cellulose and hemicellose.

  2. Clostridium perfringens type E enteritis in calves: two cases and a brief review of the literature.

    Science.gov (United States)

    Songer, J Glenn; Miskimmins, Dale W

    2004-08-01

    Toxigenic types of Clostridium perfringens are important causes of enteric disease in domestic animals, although type E is putatively rare, appearing as an uncommon cause of enterotoxemia of lambs, calves, and rabbits. We report here two geographically distinct cases of type E enterotoxemia in calves, and diagnostic findings which suggest that type E may play a significant role in enteritis of neonatal calves. The cases had many similarities, including a history of diarrhea and sudden death, abomasitis, and hemorrhagic enteritis. In both cases, anaerobic cultures of abomasum yielded heavy growth of C. perfringens genotype E. Four percent of > 1000 strains of C. perfringens from cases of enteritis in domestic animals were type E, and all (n=45) were from neonatal calves with hemorrhagic enteritis. Furthermore, type E isolates represented nearly 50% of all isolates submitted from similar clinical cases in calves. Commercial toxoids available in North America have no label claims for efficacy against type E infections. Consideration should be given to type E-associated enteritis when planning for the health care of calves.

  3. Clinicopathologic features of experimental Clostridium perfringens type D enterotoxemia in cattle.

    Science.gov (United States)

    Filho, E J F; Carvalho, A U; Assis, R A; Lobato, F F; Rachid, M A; Carvalho, A A; Ferreira, P M; Nascimento, R A; Fernandes, A A; Vidal, J E; Uzal, F A

    2009-11-01

    This study was designed to experimentally reproduce enterotoxemia by Clostridium perfringens type D in cattle and to characterize the clinicopathologic findings of this disease. Fourteen 9-month-old calves were inoculated intraduodenally according to the following schedule: group 1 (n = 4), C. perfringens type D whole culture; group 2 (n = 3), C. perfringens type D washed cells; group 3 (n = 5), C. perfringens type D filtered and concentrated supernatant; group 4 (n = 2), sterile, nontoxic culture medium. In addition, all animals received a 20% starch solution in the abomasum. Ten animals from groups 1 (4/4), 2 (3/3), and 3 (3/5) showed severe respiratory and neurologic signs. Gross findings were observed in these 10 animals and consisted of acute pulmonary edema, excessive protein-rich pericardial fluid, watery contents in the small intestine, and multifocal petechial hemorrhages on the jejunal mucosa. The brain of one animal of group 2 that survived for 8 days showed multifocal, bilateral, and symmetric encephalomalacia in the corpus striatum. The most striking histologic changes consisted of perivascular high protein edema in the brain, and alveolar and interstitial proteinaceous pulmonary edema. The animal that survived for 8 days and that had gross lesions in the corpus striatum showed histologically severe, focal necrosis of this area, cerebellar peduncles, and thalamus. Koch's postulates have been met and these results show that experimental enterotoxemia by C. perfringens type D in cattle has similar clinical and pathologic characteristics to the natural and experimental disease in sheep.

  4. Growth rate, health and welfare in a dairy herd with natural suckling until 6–8 weeks of age: a case report

    Directory of Open Access Journals (Sweden)

    Mejdell Cecilie

    2007-06-01

    Full Text Available Abstract Over a period of two years, growth rate and health were measured for dairy calves allowed to suckle their mothers up to 6–8 weeks of age. Thirty-one calves were weighted weekly, and the mean daily growth rate was 1.2 ± 0.03 kg from birth up to 13 weeks of age. Illness in calves and young stock was not observed. In the cows, the mean incidences of ketosis, displaced abomasum, puerperal paresis, mastitis, teat injury and retained placenta were 0, 0, 8, 22, 1 and 1%, respectively, during a 6-year period. The mean daily gain of 56 growing bulls was 1.4 kg when slaughtered at 15 months of age, which is higher than the mean daily gain of 0.95 kg in the population. Probiotics, hormones and vaccines were not used, and antibiotics were only used for treating illness. The present study indicates many advantages and few problems when dairy calves are penned together with the cows and allowed natural feeding up to 6–8 weeks of age. This production system was easy to manage, preferred by the farmer, and may satisfy the public concern regarding the practice of immediate separation of cow and calf in commercial milk production.

  5. Effect of ruminal vs postruminal administration of degradable protein on utilization of low-quality forage by beef steers.

    Science.gov (United States)

    Bandyk, C A; Cochran, R C; Wickersham, T A; Titgemeyer, E C; Farmer, C G; Higgins, J J

    2001-01-01

    An experiment was designed to determine the effects of ruminal and postruminal infusions of ruminally degradable protein (casein) on intake and digestion of low-quality hay by beef steers. Twelve ruminally fistulated Angus x Hereford steers (initial BW = 563 kg) were blocked by weight and assigned to one of three treatments: control (C; hay only) or hay plus ruminal (R) or postruminal (P) infusion of 400 g/d of sodium caseinate. The trial consisted of five periods: 1) 10-d adaptation to the hay diet; 2) 7-d measurement of hay intake (without infusions); 3) 10-d adaptation to protein infusion treatments (intake measurements continued); 4) 7-d measurement of hay intake and digestibility (infusions continued); and 5) 3-d ruminal sampling period (infusions continued). Steers were given ad libitum access to tallgrass-prairie hay (3.4% CP, 76.6% NDF) throughout the study. Casein was administered once daily before feeding, either directly into the rumen or via anchored infusion lines into the abomasum. Hay intake was increased by supplementation (P infusion elicited a greater (P = 0.04) increase in hay intake than postruminal infusion. Intake tended (P = 0.11) to be lower in period 4 than in period 2 for control steers but was greater in period 4 than in period 2 (P infusion of a degradable protein source improved forage utilization, although the response in forage OM intake and total digestible OM intake was greater for ruminal infusion than for postruminal infusion.

  6. Characterization of the abomasal transcriptome for mechanisms of resistance to gastrointestinal nematodes in cattle

    Directory of Open Access Journals (Sweden)

    Li Robert W

    2011-11-01

    Full Text Available Abstract The response of the abomasal transcriptome to gastrointestinal parasites was evaluated in parasite-susceptible and parasite-resistant Angus cattle using RNA-seq at a depth of 23.7 million sequences per sample. These cattle displayed distinctly separate resistance phenotypes as assessed by fecal egg counts. Approximately 65.3% of the 23 632 bovine genes were expressed in the fundic abomasum. Of these, 13 758 genes were expressed in all samples tested and likely represent core components of the bovine abomasal transcriptome. The gene (BT14427 with the most abundant transcript, accounting for 10.4% of sequences in the transcriptome, is located on chromosome 29 and has unknown functions. Additionally, PIGR (1.6%, Complement C3 (0.7%, and Immunoglobulin J chain (0.5% were among the most abundant transcripts in the transcriptome. Among the 203 genes impacted, 64 were significantly over-expressed in resistant animals at a stringent cutoff (FDR

  7. Characterization of the abomasal transcriptome for mechanisms of resistance to gastrointestinal nematodes in cattle.

    Science.gov (United States)

    Li, Robert W; Rinaldi, Manuela; Capuco, Anthony V

    2011-11-30

    The response of the abomasal transcriptome to gastrointestinal parasites was evaluated in parasite-susceptible and parasite-resistant Angus cattle using RNA-seq at a depth of 23.7 million sequences per sample. These cattle displayed distinctly separate resistance phenotypes as assessed by fecal egg counts. Approximately 65.3% of the 23,632 bovine genes were expressed in the fundic abomasum. Of these, 13,758 genes were expressed in all samples tested and likely represent core components of the bovine abomasal transcriptome. The gene (BT14427) with the most abundant transcript, accounting for 10.4% of sequences in the transcriptome, is located on chromosome 29 and has unknown functions. Additionally, PIGR (1.6%), Complement C3 (0.7%), and Immunoglobulin J chain (0.5%) were among the most abundant transcripts in the transcriptome. Among the 203 genes impacted, 64 were significantly over-expressed in resistant animals at a stringent cutoff (FDR gastrointestinal nematode infection and will facilitate understanding of mechanism underlying host resistance.

  8. First description of gastrointestinal nematodes of Barbary sheep (Ammotragus lervia): the case of Camelostrongylus mentulatus as a paradigm of phylogenic and specific relationship between the parasite and its ancient host.

    Science.gov (United States)

    Mayo, E; Ortiz, J; Martínez-Carrasco, C; Garijo, M M; Espeso, G; Hervías, S; Ruiz de Ybáñez, M R

    2013-09-01

    The gastrointestinal helminth fauna of 24 Barbary sheep or Aoudad (Ammotragus lervia sahariensis) maintained in the Parque de Rescate de la Fauna Sahariana (PRFS, CSIC, Almeria, Spain) was analyzed. Most animals (87.5 %) were parasitized, and multiple infections were highly present. The following species were identified: Camelostrongylus mentulatus, Teladorsagia circumcincta, Marshallagia marshalli, Ostertagia ostertagi, O. leptospicularis, O. lyrata, Haemonchus contortus, Teladorsagia trifurcata, Trichostrongylus vitrinus, T. colubriformis, T. probolorus, T. capricola, Nematodirus spathiger, N. abnormalis, N. filicollis, N. helvetianus, Trichuris spp. and Skrjabinema ovis. Teladorsagia circumcincta was the most prevalent nematode in abomasum (52.6 %) followed by C. mentulatus (50 %). However, this latter nematode had the greater mean intensity and abundance. In the small intestine, T. colubriformis and T. vitrinus had the highest prevalence (36.4 %); the last one showed also the greater mean intensity and abundance. It should be emphasized the presence of Skrjabinema ovis (prevalence 39.1 %) in the large intestine, showing the greater mean abundance and intensity, although with a low values. Camelostrongylus mentulatus could be the most primitive nematode of the family trichostrongylidae recovered in this study; attending to its high prevalence, mean abundance and mean intensity, the possible specificity between this parasite and the Aoudad is discussed.

  9. Serum calcium and magnesium level in dairy cows at calving

    Directory of Open Access Journals (Sweden)

    A.M. Pulimeno

    2011-03-01

    Full Text Available Milk fever and hypocalcaemia are post-partum metabolic diseases affecting about 6% of dairy cows and are due to a fail of the homeostatic metabolism regulating the calcium blood level around 9 and 10mg/100mL. The calcium drainage to the mammary gland along with the reduced capacity of the animal to mobilize calcium from bone reserve lead to a drop of the calcium blood level under 5-6mg/100mL with paresis like clinical symptoms known as milk fever. The incidence of the clinical milk fever is low, however the occurrence of mild hypocalcaemia (subclinical could be as high as 15- 20% within few days after calving, particularly in multiparous cows. The hypocalcaemia status as for the reduced bone calcium mobilization and intestinal absorption leads to reduced feed intake and make it a good start for ketosis, retained placenta, displaced abomasums and mastitis problems (Beede, 1991. The acid-base balance of the cow in the late pregnancy is determinant for hypocalcaemia............

  10. High mortality due to accidental salinomycin intoxication in sheep

    Directory of Open Access Journals (Sweden)

    Ashrafihelan Javad

    2014-09-01

    Full Text Available In February 2012, 100% mortality was reported in a herd with 79 local sheep that were kept around of Abhar, Northwest of Iran. The ration for adult sheep was daily mixed (40 kg straw, 25 kg wheat and 2 kg Vit-C premix and accidentally 1 500 g of salinomycin (Salinomycin 12% Premix; Aras Bazar Laboratories, Iran had been added to the ration (22388 mg/kg = 22388 ppm and overnight was fed to herd. At the morning, 78 sheep were founded dead and one of them showed convulsive seizures. Postmortem examination revealed pulmonary congestion and edema, hemorrhages in abomasum, large pale kidney and white streak lines in myocardium. Main histopathologic lesions were extensive subepicardial and intercardiomyofibers hemorrhages, extensive cardiomyolysis and myocarditis in heart, severe hyperemia and extensive acute tubular necrosis (ATN in kidneys and focal necrosis and retention of bile cholangitis in the liver. In this study, on the basis of the history, observation of the ionophore remnant in the ration, clinical signs, gross and histopathological findings, acute salinomycin intoxication is definitely diagnosed.

  11. Cloning of the Authentic Bovine Gene Encoding Pepsinogen A and Its Expression in Microbial Cells

    Science.gov (United States)

    Muñoz, Rosario; García, José L.; Carrascosa, Alfonso V.; Gonzalez, Ramon

    2004-01-01

    Bovine pepsin is the second major proteolytic activity of rennet obtained from young calves and is the main protease when it is extracted from adult animals, and it is well recognized that the proteolytic specificity of this enzyme improves the sensory properties of cheese during maturation. Pepsin is synthesized as an inactive precursor, pepsinogen, which is autocatalytically activated at the pH of calf abomasum. A cDNA coding for bovine pepsin was assembled by fusing the cDNA fragments from two different bovine expressed sequence tag libraries to synthetic DNA sequences based on the previously described N-terminal sequence of pepsinogen. The sequence of this cDNA clearly differs from the previously described partial bovine pepsinogen sequences, which actually are rabbit pepsinogen sequences. By cloning this cDNA in different vectors we produced functional bovine pepsinogen in Escherichia coli and Saccharomyces cerevisiae. The recombinant pepsinogen is activated by low pH, and the resulting mature pepsin has milk-clotting activity. Moreover, the mature enzyme generates digestion profiles with α-, β-, or κ-casein indistinguishable from those obtained with a natural pepsin preparation. The potential applications of this recombinant enzyme include cheese making and bioactive peptide production. One remarkable advantage of the recombinant enzyme for food applications is that there is no risk of transmission of bovine spongiform encephalopathy. PMID:15128507

  12. Stomach lysozymes of the three-toed sloth (Bradypus variegatus), an arboreal folivore from the Neotropics.

    Science.gov (United States)

    Pacheco, M Andreína; Concepción, Juan Luís; Rangel, José David Rosales; Ruiz, Marie Christine; Michelangeli, Fabián; Domínguez-Bello, María G

    2007-07-01

    Lysozymes are antimicrobial defences that act as digestive enzymes when expressed in the stomach of herbivores with pre-gastric fermentation. We studied this enzyme in the complex stomach of the three-toed sloth (Bradypus variegatus), a folivore with pre-gastric fermentation. Lysozymes were identified by SDS-PAGE and immunoblotting in all portions: diverticulum, pouch, glandular and muscular prepyloric area with 14.3 kDa of molecular mass. Purified lysozymes from all areas but the diverticulum were characterized by MALDI-TOF, optimal pH, optimal ionic strength, and specific activity. The differences observed suggested at least three isoforms. The optimal pHs were similar to the pH of the stomach portion where the enzymes were isolated. The lysozyme from the pouch (fermentation chamber) exhibited higher specific activity and concentration than the others. The specific activity of the enzyme from the acid muscular prepyloric portion was comparable to that reported in the cow abomasums; however, its concentration was lower than that observed in cow. This distinctive pattern of secretion/specific activity and overall low concentration suggests different roles for the lysozymes in this herbivore compared to Artiodactyla. We postulate that sloth stomach lysozymes may still be antimicrobial defences by protecting the microbial flora of the fermentation chamber against foreign bacteria.

  13. INTOXICAÇÃO NATURAL DE BOVINOS LEITEIROS POR Cestrum laevigatum (SOLANACEAE NO AGRESTE DE PERNAMBUCO – BRASIL

    Directory of Open Access Journals (Sweden)

    Luiz Teles Coutinho

    2013-09-01

    Full Text Available The aim of the present study was to report an outbreak of natural poisoning by Cestrum laevigatum in dairy cattle in the “Agreste” region of Pernambuco state, Brazil. Epidemiological and clinical data were collected. Among a lot of 60 cows, eight became ill and four died. Two cows underwent necropsy, during which fragments of the central nervous system, liver, gall bladder, spleen and kidney were collected for histopathlogical analysis. Blood samples were collected for hematological and biochemical tests. The animals exhibited apathy, muscle tremors, reduced appetite, different degrees of dehydration and compromised reticulorumen dynamics as well as a small quantity of dry feces with the presence of mucus and blood. The laboratory exams revealed an increase in serum activity of aspartate aminotransferase and gamma glutamyltransferase as well as hypoalbuminemia. The necropsy revealed an enlarged liver and cutting surface with a nutmeg aspect as well as areas of hemorrhaging in the heart, trachea, abomasum, spleen, intestine and bladder. The microscopic analysis revealed centrilobular hepatic necrosis associated to accentuated hemorrhaging. These findings characterized poisoning by Cestrum laevigatum and led to the adoption of control and prevention measures.

  14. Increased blood concentration of isopropanol in ketotic dairy cows and isopropanol production from acetone in the rumen.

    Science.gov (United States)

    Sato, Hiroshi

    2009-08-01

    To evaluate acetone and isopropanol metabolism in bovine ketosis, the blood concentrations of isopropanol, acetone, plasma 3-hydroxybutyrate (3-HB) and other metabolites were analyzed in 12 healthy controls and 15 ketotic dairy cows including fatty liver and inferior prognosis after laparotomy for displaced abomasum. In ruminal fluid taken from 6 ketotic cows, ruminal isopropanol and acetone were also analyzed. Ketotic cows showed higher concentrations of isopropanol, acetone, 3-HB and nonesterified fatty acid, and higher activities of aspartate transaminase and gamma-glutamyl transferase than control cows. Blood samples had higher concentration of isopropanol accompanied by increased acetone. In the ketotic cows, acetone was detected not only in blood but also in ruminal fluid, while higher ruminal isopropanol did not necessarily accompany its elevation in the blood. Using 2 steers with rumen cannula, all ruminal content was emptied and then substituted with artificial saliva to evaluate the importance of ruminal microbes in isopropanol production. Under each condition of intact and emptied rumen, acetone was infused into the rumen and blood isopropanol was analyzed. The elevation in the blood isopropanol concentration after acetone infusion was markedly inhibited by the emptying. Here, increased blood concentrations of isopropanol and acetone were observed in ketotic cows, and the importance of ruminal microbes in isopropanol production was confirmed.

  15. Metal exposure in cows grazing pasture contaminated by iron industry: Insights from magnetic particles used as tracers.

    Science.gov (United States)

    Ayrault, Sophie; Catinon, Mickaël; Boudouma, Omar; Bordier, Louise; Agnello, Gregory; Reynaud, Stéphane; Tissut, Michel

    2016-05-01

    Magnetic particles (MP) emitted by an iron smelter were used to investigate the exposure of cows grazing on a grassland polluted by these MP and by large amounts of potentially toxic elements (PTE). The morphology as well as the chemical composition of the MP separated from cow dung were studied. Large amounts of typical MP were found (1.1 g kg(-1) dry weight) in the cow dung sampled from the exposed site, whereas these particles were absent from the reference unpolluted site. The ingested MP were mainly technogenic magnetic particles (TMP) emitted by the smelter. Considering the MP concentration in the grazed grass on the exposed site, it was concluded that cows absorb the MP not only from the grass but also from the soil surface. The results of a mild acidic leaching of the MP suggested that the particles were possibly submitted to a superficial dissolution in the abomasum, pointing at a potential route of transfer of the PTE originating from the TMP and leading into food chains. TMP were only a small part of the anthropogenic contamination having affected the soil and the dung. However, due to their unequivocal signature, TMP are a powerful tracer of the distribution of PTE in the different compartments constituting the food chains and the ecosystems. Furthermore, the measurement of the particle sizes gave evidence that a noticeable proportion of the MP could enter the respiratory tract.

  16. Characterising the bacterial microbiota across the gastrointestinal tracts of dairy cattle: membership and potential function.

    Science.gov (United States)

    Mao, Shengyong; Zhang, Mengling; Liu, Junhua; Zhu, Weiyun

    2015-11-03

    The bacterial community composition and function in the gastrointestinal tracts (GITs) of dairy cattle is very important, since it can influence milk production and host health. However, our understanding of bacterial communities in the GITs of dairy cattle is still very limited. This study analysed bacterial communities in ten distinct GIT sites (the digesta and mucosa of the rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon and rectum) in six dairy cattle. The study observed 542 genera belonging to 23 phyla distributed throughout the cattle GITs, with the Firmicutes, Bacteroidetes and Proteobacteria predominating. In addition, data revealed significant spatial heterogeneity in composition, diversity and species abundance distributions of GIT microbiota. Furthermore, the study inferred significant differences in the predicted metagenomic profiles among GIT regions. In particular, the relative abundances of the genes involved in carbohydrate metabolism were overrepresented in the digesta samples of forestomaches, and the genes related to amino acid metabolism were mainly enriched in the mucosal samples. In general, this study provides the first deep insights into the composition of GIT microbiota in dairy cattle, and it may serve as a foundation for future studies in this area.

  17. Bovine leukemia virus high tax molecular clone experimentally induces leukemia/lymphoma in sheep.

    Science.gov (United States)

    Okada, Kosuke; Nakae, Norihiro; Kuramochi, Konomi; Yin, Shan-ai; Ikeda, Manabu; Takami, Shigeaki; Hirata, Tou-ichi; Goryo, Masanobu; Numakunai, Shigeru; Takeshima, Shin-nosuke; Takahashi, Masahiko; Tajima, Shigeru; Konnai, Satoru; Onuma, Misao; Aida, Yoko

    2005-12-01

    Sheep were inoculated with high tax coded pBLV-IF (H group, Nos.1-5) of bovine leukemia virus (BLV), wild tax coded pBLV-IF (W group, Nos. 6-11), or control plasmid (C group, Nos. 12-14). During the observation period (4 to 46 months), 5 of 5 cases in H group and 3 of 6 cases (Nos. 6, 7, 9) in W group became positive for gp 51. Only 1 case in H group became leukemic, and one case each of H and W groups developed lymphoma. In No. 3, lesions were found in multiple organs including the lymph nodes, gastrointestinal tract following abomasum, and heart. In No. 6, lesions of lymphoma were found only in the jejunum and heart. Morphologically, small to middle-sized lymphocytic neoplastic (NP) cells were found in both cases, but lymphoblastic NP cells were found only in No. 3. By immunohistochemical examination, the phenotypes of NP cells were determined as CD1-, CD4-, CD5- -, CD8alpha-, sIgM+, lambda light chain+, B-B4+, MHC class II+ in both case. The results of this study indicate that inoculation of pBLV-IF can induce lymphocytic and lymphoblastic leukemia/lymphoma in sheep. Additionally, it is suggested that the expression rate of tax gene is not associated with the development of leukemia/lymphoma in sheep experimentally inoculated with pBLV-IF.

  18. Biotransformation of albendazole and activities of selected detoxification enzymes in Haemonchus contortus strains susceptible and resistant to anthelmintics.

    Science.gov (United States)

    Vokřál, Ivan; Jirásko, Robert; Stuchlíková, Lucie; Bártíková, Hana; Szotáková, Barbora; Lamka, Jiří; Várady, Marián; Skálová, Lenka

    2013-09-23

    The increased activity of drug-metabolizing enzymes can protect helminths against the toxic effect of anthelmintics. The aim of this study was to compare the metabolism of the anthelmintic drug albendazole (ABZ) and the activities of selected biotransformation and antioxidant enzymes in three different strains of Haemonchus contortus: the ISE strain (susceptible to common anthelmintics), the BR strain (resistant to benzimidazole anthelmintics) and the WR strain (multi-resistant). H. contortus adults were collected from the abomasum of experimentally infected lambs. In vitro (subcellular fractions of H. contortus homogenate) as well as ex vivo (living nematodes cultivated in flasks with medium) experiments were performed. HPLC with spectrofluorimetric and mass-spectrometric detection was used in the analysis of ABZ metabolites. The in vitro activities of oxidation/antioxidation and conjugation enzymes toward model substrates were also assayed. The in vitro data showed significant differences between the susceptible (ISE) and resistant (BR, WR) strains regarding the activities of peroxidases, catalase and UDP-glucosyltransferases. S-oxidation of ABZ was significantly lower in BR than in the ISE strain. Ex vivo, four ABZ metabolites were identified: ABZ sulphoxide and three ABZ glucosides. In the resistant strains BR and WR, the ex vivo formation of all ABZ glucosides was significantly higher than in the susceptible ISE strain. The altered activities of certain detoxifying enzymes might partly protect the parasites against the toxic effect of the drugs as well as contribute to drug-resistance in these parasites.

  19. Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortus

    Directory of Open Access Journals (Sweden)

    Donnan Alison A

    2011-03-01

    Full Text Available Abstract Background The cysteine proteinases in papaya latex have been shown to have potent anthelmintic properties in monogastric hosts such as rodents, pigs and humans, but this has not been demonstrated in ruminants. Methods In two experiments, sheep were infected concurrently with 5,000 infective larvae of Haemonchus contortus and 10,000 infective larvae of Trichostrongylus colubriformis and were then treated with the supernatant from a suspension of papaya latex from day 28 to day 32 post-infection. Faecal egg counts were monitored from a week before treatment until the end of the experiment and worm burdens were assessed on day 35 post-infection. Results We found that the soluble fraction of papaya latex had a potent in vivo effect on the abomasal nematode H. contortus, but not on the small intestinal nematode T. colubriformis. This effect was dose-dependent and at tolerated levels of gavage with papaya latex (117 μmol of active papaya latex supernatant for 4 days, the H. contortus worm burdens were reduced by 98%. Repeated treatment, daily for 4 days, was more effective than a single dose, but efficacy was not enhanced by concurrent treatment with the antacid cimetidine. Conclusions Our results provide support for the idea that cysteine proteinases derived from papaya latex may be developed into novel anthelmintics for the treatment of lumenal stages of gastro-intestinal nematode infections in sheep, particularly those parasitizing the abomasum.

  20. Subchronic centrifugal mechanical assist in an unheparinized calf model.

    Science.gov (United States)

    Wagner-Mann, C; Curtis, J; Mann, F A; Turk, J; Demmy, T; Turpin, T

    1996-06-01

    The purpose of this study was to determine whether the major centrifugal pumps currently in use in the United States (Medtronic, BioMedicus BioPump and Carmeda-coated BioPump, Sarns 3M centrifugal pump, and St. Jude Medical Lifestream) could function as left mechanical assist devices in the subchronic (72 h) unheparinized calf model. Calves were instrumented for left atrial to aorta ex vivo assist, and the pump flow was set at 3.5 +/- 0.4 L/min. Two calves (Sarns 3M and St. Jude) survived 72 h of pumping without clinical complications. The other 2 calves died at 62 and 66 h (Medtronic BioPump and Carmeda-coated BioPump, respectively); both had pelvic limb paralysis. The seal of the Sarns 3M pump head ruptured after approximately 36 h of pumping and required replacement. On postmortem examination, pump-associated thromboembolic lesions were detected in 3 of the 4 calves in one or more of the following organs: kidneys, pancreas, abomasum, duodenum, ileum, spleen, and lumbar spinal cord. The calf with the Sarns 3M pump had no discernable lesions. Because of the clinical abnormalities and postmortem lesions in the unheparinized calf model, it was suggested that anticoagulation is necessary for conducting centrifugal mechanical assist studies in calves using presently available technology.

  1. Experimental Clostridium perfringens type D enterotoxemia in goats.

    Science.gov (United States)

    Uzal, F A; Kelly, W R

    1998-03-01

    The effects of intraduodenal administration of Clostridium perfringens cultures and culture products in goats were evaluated to develop a reliable experimental model of enterotoxemia in this species. Five conventionally reared, 11-16-week-old Angora goat kids were dosed intraduodenally with whole cultures of C. perfringens type D; five similar animals were dosed with C. perfringens type D filtered culture supernatant; and a third group of five kids was dosed with C. perfringens type D washed cells. Two kids were used as controls and received sterile, nontoxic culture medium intraduodenally. All animals received starch solution into the abomasum. All five kids inoculated with whole culture and three of five dosed with culture supernatant and with washed cells developed central nervous system signs. Diarrhea was observed in two of five kids inoculated with whole culture, in all five of those dosed with culture supernatant, and in three of five of those that received washed cells. The most striking postmortem findings consisted of lung edema, necrotizing pseudomembranous colitis, and cerebral vasogenic edema. The protocol thus provided a reasonable model of naturally occurring enterotoxemia in goats, producing a range of clinical signs and postmortem changes similar to those observed in the natural disease.

  2. Seneciosis in cattle associated with photosensitization

    Directory of Open Access Journals (Sweden)

    Paula R. Giaretta

    2014-05-01

    Full Text Available Senecio spp. poisoning is the main cause of cattle mortality in the central region of Rio Grande do Sul. This paper reports an outbreak of seneciosis in cattle with high prevalence of photosensitization, where 83 out of 162 cows (51.3% presented this clinical sign. The outbreak occurred in September 2013, affecting adult cows that were held in a 205 hectare-pasture from April to October 2013 with abundant Senecio brasiliensis infestation. Main clinical signs were weight loss, excessive lacrimation or mucopurulent ocular discharge, nasal serous discharge, ventral diphteric glossitis, crusts in the nose, teats, dorsum of ears, and vulva. Liver biopsy was performed in all the cows under risk; the histopathological findings in the liver biopsies consisted of fibrosis, megalocytosis, and biliary ductal proliferation and were present in 73.4% of the biopsied animals. Six cows had increased serum activity of gamma glutamyl transferase. Three affected cows were necropsied. The main necropsy findings were a hard liver, distended gall bladder, edema of the mesentery and abomasum. Liver histological changes in the necropsied cows were similar to those of the biopsied livers. Spongiosis was detected in the brain of necropsied cows and is characteristic of hepatic encephalopathy.

  3. Replacement of Forage Fiber Sources with Dried Distillers Grains with Solubles and Corn Germ Meal in Holstein Calf Diets

    Institute of Scientific and Technical Information of China (English)

    XU Jun; HOU Yu-jie; ZHAO Guo-qi; YU Ai-bing; SU Yan-jing; HUO Yong-jiu; ZHU Jian-ming

    2014-01-01

    This study was carried out to determine the effect of replacement of forage ifber sources from alfalfa and Leymus chinensis with nonforage ifber sources (NFFS) from dried distillers grains with solubles and corn germ meal on calves growth, rumen development and blood parameters. 48 female and 12 male calves ((110.55±15.36) kg of body weight and 12 wk of age) were assigned randomly to four dietary treatments (15 calves/treatment) in a completely randomized design. Experimental diets were:0%NFFS (control), 9%NFFS (group 1), 18%NFFS (group 2), 27%NFFS (group 3), and contained equivalent neutral detergent ifber and total digestible nutrients, respectively. The dry matter intake was similar among diets, averaging 3.33 kg d-1, and no differences were detected for body weight, withers height, body length and heart girth. In addition, the development of rumen, reticulum, omasum and abomasum also were similar among diets. Dry matter, crude protein, and neutral detergent ifber digestibilities increased with the increasing levels of NFFS in the experimental diets, but had no signiifcant effect. Blood urea nitrogen, total protein and glubulin were not affected by the dietary treatment, but group 3 resulted in the highest (P<0.05) concentrations of glucose and the lowest (P<0.05) concentrations of triglycerides and albumin. In conclusion, dried distillers grains with solubles (DDGS) and corn germ meal (CGM) were available and alternative ifber sources for Holstein calf diets.

  4. THE EFFECTS OF ORAL ADMINISTRATION OF PROPYLENE GLYCOL AND CALCIUM PROPIONATE IN DAIRY COWS

    Directory of Open Access Journals (Sweden)

    C. GAVAN

    2013-07-01

    Full Text Available This study was designed to determine the effects of the oral administration of propylene glycol and calcium propionate on performance of dairy cows. Treatments were 10 l water (control, 10 l water+300 ml propylene glycol (GP and 10 l water+500 g calcium propionate (CP. Animals were mainly of Holstein breeds and were fed and managed in a commercial setting. The cows were divided randomly into an experimental group, n=24 (n=12 with PG and n=12 with CP and a control group, n=11. Cows received the assigned treatment within 10 hours of calving and 24 hours after calving. Health events were recorded during calving and for the first 21 days in milk (DIM. Health examinations were performed on cows that appeared not well. The cows were milked three times daily and milk production was recorded electronically. Milk solid content and somatic cell score were determinate from three consecutive milking weekly till 20 DIM and than monthly till 110 DIM. Retained placenta, hypocalcaemia, displaced abomasums, ketosis and metritis were low in treatment groups (with PG and CP. The cows receiving PG had 2.8 Kg/day grater milk production than control group. The cows receiving CP had 1.7 kg/day grater milk production than control group. Prophylactic administration of PG and CP drenches to Holstein cows may be justified by potentially higher milk yields and reduced health complications.

  5. Computed tomography of the abdomen of calves during the first 105 days of life: II. Liver, spleen, and small and large intestines.

    Science.gov (United States)

    Braun, U; Schnetzler, C; Augsburger, H; Müller, U; Dicht, S; Ohlerth, S

    2014-05-01

    Computed tomography (CT) findings of the liver, spleen and intestines of five healthy calves during six examinations in the first 105 days of life were compared with corresponding cadaver slices. The liver was located in the right hemiabdomen adjacent to the diaphragm and right abdominal wall. The caudal vena cava was seen dorsomedially and the portal vein further ventrally. The umbilical vein was seen running from the navel to the liver in all calves in the first scan and in four calves in the second scan. The spleen ran dorsoventrally adjacent to the costal part of the left abdominal wall and appeared sickle-shaped on transverse images. Differentiation of small and large intestines was only possible when the former contained fluid content and the latter gaseous content. The small intestine was in the left hemiabdomen dorsal to the abomasum and caudodorsal to the rumen at the first two examinations. Growth of the forestomachs caused displacement of the small intestine to the right and toward the ventral abdomen caudal to the liver and adjacent to the right abdominal wall. The large intestine was located caudodorsally, and the typical features of the spiral colon were apparent in the dorsal plane. The location of the caecum varied from dorsal to the spiral colon to adjacent to the right abdominal wall with the apex always pointing caudally. The rectum was easily identified in the pelvic region. The size, volume and density of the described organs throughout the study are shown in several tables.

  6. Enteric listeriosis in grazing steers supplemented with spoiled silage.

    Science.gov (United States)

    García, Juan A; Micheloud, Juan F; Campero, Carlos M; Morrell, Eleonora L; Odriozola, Ernesto R; Moreira, Ana R

    2016-01-01

    An outbreak of enteric listeriosis in steers that were fed spoiled silage is reported. The outbreak started 2 days after ~200 animals in a single paddock were given a supplement of spoiled silage. Forty animals (20%) were affected, and 13 (6.5%) died over a period of 10 days. Affected animals were recumbent, depressed, and had diarrhea with mucus and fibrin. Gross and microscopic findings in 3 animals that were subjected to autopsy included excess peritoneal fluid, congestion and edema of abomasum, suppurative enteritis and colitis, and suppurative mesenteric lymphadenitis. Two strains of Listeria monocytogenes were isolated, one of serotype 1/2c from the gallbladder and one of serotype 1/2b from the spoiled silage. Listeria monocytogenes was detected in the mesenteric lymph nodes and intestinal wall of 1 animal by immunohistochemistry (IHC). Clinical history and signs, gross and microscopic findings, bacterial isolation, and IHC results confirmed a diagnosis of enteric listeriosis. The source of infection was likely the spoiled silage.

  7. UT-B Urea Transporter Localization in the Bovine Gastrointestinal Tract.

    Science.gov (United States)

    Coyle, J; McDaid, S; Walpole, C; Stewart, Gavin S

    2016-04-01

    Facilitative UT-B urea transporters play an important role in the urea nitrogen salvaging process that occurs in the gastrointestinal tract of mammals, particularly ruminants. Gastrointestinal UT-B transporters have previously been reported in various ruminant species-including cow, sheep and goat. In this present study, UT-B transporter localization was investigated in tissues throughout the bovine gastrointestinal tract. RT-PCR analysis showed that UT-B2 was the predominant UT-B mRNA transcript expressed in dorsal, ventral and cranial ruminal sacs, while alternative UT-B transcripts were present in other gastrointestinal tissues. Immunoblotting analysis detected a strong, glycosylated ~50 kDa UT-B2 protein in all three ruminal sacs. Immunolocalization studies showed that UT-B2 protein was predominantly localized to the plasma membrane of cells in the stratum basale layer of all ruminal sac papillae. In contrast, other UT-B protein staining was detected in the basolateral membranes of the surface epithelial cells lining the abomasum, colon and rectum. Overall, these findings confirm that UT-B2 cellular localization is similar in all ruminal sacs and that other UT-B proteins are located in epithelial cells lining various tissues in the bovine gastrointestinal tract.

  8. Potential and existing mechanisms of enteric methane production in ruminants

    Directory of Open Access Journals (Sweden)

    Junyi Qiao

    2014-10-01

    Full Text Available Enteric methane (CH4 emissions in ruminants have attracted considerable attention due to their impact on greenhouse gases and the contribution of agricultural practices to global warming. Over the last two decades, a number of approaches have been adopted to mitigate CH4 emissions. However, the mechanisms of methanogenesis have still not been fully defined. According to the genome sequences of M. ruminantium in the rumen and of M. AbM4 in the abomasum, the pathways of carbon dioxide (CO2 reduction and formate oxidation to CH4 have now been authenticated in ruminants. Furthermore, in the light of species or genera description of methanogens, the precursors of methanogenesis discovered in the rumen and research advances in related subjects, pathways of acetate dissimilation via Methanosarcina and Methanosaeta as well as metabolism of methanol to CH4 might be present in the rumen, although neither process has yet been experimentally demonstrated in the rumen. Herein the research advances in methanogenesic mechanisms including existing and potential mechanisms are reviewed in detail. In addition, further research efforts to understand the methanogenesis mechanism should focus on isolation and identification of more specific methanogens, and their genome sequences. Such increased knowledge will provide benefits in terms of improved dietary energy utilization and a reduced contribution of enteric CH4 emissions to total global greenhouse gas emissions from the ruminant production system.

  9. Effects of mineral content of bovine drinking water: does iron content affect milk quality?

    Science.gov (United States)

    Mann, G R; Duncan, S E; Knowlton, K F; Dietrich, A D; O'Keefe, S F

    2013-01-01

    The composition of water given to dairy cattle is often ignored, yet water is a very important nutrient and plays a major role in milk synthesis. The objective of this study was to study effects of elevated levels of iron in bovine drinking water on milk quality. Ferrous lactate treatments corresponding to 0, 2, 5, and 12.5mg/kg drinking water concentrations were delivered through the abomasum at 10 L/d to 4 lactating dairy cows over 4 periods (1 wk infusion/period) in a Latin square design. On d 6 of infusion, milk was collected, processed (homogenized, pasteurized), and analyzed. Mineral content (Fe, Cu, P, Ca) was measured by inductively coupled plasma mass spectrometry. Oxidative stability of whole processed milk was measured by the thiobarbituric acid reactive substances (TBARS) assay for malondialdehyde (MDA) and sensory analysis (triangle test) within 72 h of processing and after 7d of storage (4°C). Significant sensory differences between processed milks from cows receiving iron and the control infusion were observed. No differences in TBARS (1.46±0.04 mg of MDA/kg) or mineral content (0.22±0.01 mg/kg Fe) were observed. A 2-way interaction (iron treatment by cow) for Ca, Cu, and Fe concentrations was seen. While iron added directly to milk causes changes in oxidation of milk, high levels of iron given to cattle have subtle effects that initially may not be obvious.

  10. Consumo e digestibilidades aparentes totais e parciais de rações contendo diferentes níveis de concentrado, em novilhos F1 Limousin x Nelore Intake and total and partial apparent digestibilities of diets with different concentrate levels, in F1 Limousin x Nellore Bulls

    Directory of Open Access Journals (Sweden)

    Rodrigo Carvalho Cardoso

    2000-12-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos de cinco níveis de concentrados (25,0; 37,5; 50,0; 62,5; e 75,0% sobre os consumos e as digestibilidades aparentes totais e parciais da matéria seca (MS, matéria orgânica (MO, proteína bruta (PB, do extrato etéreo (EE, dos carboidratos totais (CHO e da fibra em detergente neutro (FDN. Cinco novilhos F1 Limousin x Nelore, não-castrados, com peso médio inicial de 279 kg, fistulados no rúmen, abomaso e íleo, foram usados em um delineamento quadrado latino 5 x 5, com cinco tratamentos, cinco períodos experimentais e cinco animais. Cada período teve duração de 14 dias - 10 dias para adaptação dos animais à ração e quatro dias para coleta de fezes e de digestas de abomaso e íleo. Utilizou-se o óxido crômico em duas doses de 10 g/dia, como indicador, para medir os fluxos de nutrientes no trato digestivo. Os consumos de EE e NDT aumentaram e o de FDN diminuiu linearmente com o incremento dos níveis de concentrados das rações. O aumento de níveis de concentrado na MS da ração resultou em acréscimos lineares nas digestilidades totais de MS, MO, PB, EE e CHO. A digestibilidade aparente total de FDN e as digestões aparentes ruminais de MS, MO, PB, CHO e FDN não foram influenciadas pelos níveis de concentrado nas rações, observando-se médias de 45,91; 54,45; 64,67; -9,92; 79,29; e 95,90%, respectivamente. Conclui-se que o aumento dos níveis de concentrado nas rações não alterou os locais de digestão dos nutrientes.The objective of this work was to evaluate the effects of five dietary concentrate levels (25.0, 37.5, 50.0, 62.5, and 75.0% on intake and the total and partial apparent digestibilities of dry matter (DM, organic matter (OM, crude protein (CP, ether extract (EE, total carbohydrates (CHO and neutral detergent fiber (NDF. Five rumen, abomasum and ileum fistulated F1 Limousin x Nellore bulls, with average initial weight of 279 kg were used, in a 5 x 5 Latin square

  11. Effects of gastrointestinal nematode infection on the ruminant immune system.

    Science.gov (United States)

    Gasbarre, L C

    1997-11-01

    Gastrointestinal (GI) nematodes of ruminants evoke a wide variety of immune responses in their hosts. In terms of specific immune responses directed against parasite antigens, the resulting immune responses may vary from those that give strong protection from reinfection after a relatively light exposure (e.g. Oesophagostomum radiatum) to responses that are very weak and delayed in their onset (e.g. Ostertagia ostertagi). The nature of these protective immune responses has been covered in another section of the workshop and the purpose of this section will be to explore the nature of changes that occur in the immune system of infected animals and to discuss the effect of GI nematode infections upon the overall immunoresponsiveness of the host. The discussion will focus primarily on Ostertagia ostertagi because this parasite has received the most attention in published studies. The interaction of Ostertagia and the host immune system presents what appears to be an interesting contradiction. Protective immunity directed against the parasite is slow to arise and when compared to some of the other GI nematodes, is relatively weak. Although responses that reduce egg output in the feces or increase the number of larvae undergoing inhibition may occur after a relatively brief exposure (3-4 months), immune responses which reduce the number of parasites that can establish in the host are not evident until the animal's second year. Additionally, even older animals that have spent several seasons on infected pastures will have low numbers of Ostertagia in their abomasa, indicating that sterilizing immune responses against the parasite are uncommon. In spite of this apparent lack of specific protective immune responses, infections with Ostertagia induce profound changes in the host immune system. These changes include a tremendous expansion of both the number of lymphocytes in the local lymph nodes and the number of lymphoid cells in the mucosa of the abomasum. This expansion

  12. Componentes corporais e órgãos internos de cordeiros Texel x Bergamácia, Texel x Santa Inês e Santa Inês puros, terminados em confinamento, com casca de café como parte da dieta Body components and internal organs of Texel x Bergamácia, Texel x Santa Inês and purebred Santa Inês lambs finished in fedlot, with of coffee hull as part of the diet

    Directory of Open Access Journals (Sweden)

    Iraides Ferreira Furusho-Garcia

    2003-12-01

    Full Text Available Foram utilizados 12 cordeiros cruzas Texel x Bergamácia (TB, 12 cordeiros cruzas Texel x Santa Inês (TS e 12 cordeiros puros Santa Inês (SI, confinados individualmente por 50 dias (dos 130 aos 180 dias de idade. Cada grupo genético constou de seis machos inteiros e seis fêmeas, recebendo três dietas diferentes: 1 = sem casca de café (controle; 2 = com casca de café in natura; 3 = com casca de café tratada com uréia. O rúmen/retículo (RR dos animais que receberam as dietas 2 e 3 foram mais pesados em relação ao RR dos animais submetidos a dieta 1. O abomaso (ABO, fígado (FIG e pâncreas (PAN dos cordeiros alimentados com a dieta 3 foram mais leves comparados aos que receberam as dietas 1 e 2. Os cordeiros cruzas TB e TS apresentaram maior peso para a cabeça (CAB, pés/canelas (PEC, omaso (OMA, ABO, intestinos delgado (IND e grosso (ING. Os machos apresentaram sangue (SAN e RR mais pesados que as fêmeas. Os pesos de pele (PEL, gordura (GOR, esôfago/traquéia (ET, coração (COR e pulmão (PUL também foram analisados e não foram afetados pelas dietas, pelos grupos genéticos e pelo sexo. As fêmeas apresentaram tendência de maior proporção de GOR.Twelve crossbred Texel x Bergamácia (TB, 12 crossbred Texel x Santa Inês (TS and 12 purebred Santa Inês (SI lambs were individually finished in fedlot for 50 days (from 130 to 180 days of age. Each genetic group consisted of six males and six females, fed three different diets: 1 = without coffee hulls (control; 2 = with coffee hulls in nature; 3 = with coffee hulls treated of with urea. The rumen/reticulum (RR of the animals fed diets 1 and 3 were significantly heavier than those fed diet 1. The abomasum (ABO, liver (FIG and pancreas (PAN of the lambs fed diet 3 were lighter than the animals fed diet 2. The TB and TS of crossbred lambs showed greater weights of head (CAB, foot/shin (PEC, omasum (OMA, abomasum (ABO, small intestine (IND and large intestine (ING. The males showed

  13. Differential expression of Orexin A in gastrointestinal tract betw een yak and cattle%Orexin A在牦牛和黄牛消化道中的表达差异

    Institute of Scientific and Technical Information of China (English)

    丁艳平; 孟媛; 张晋平; 邵宝平

    2016-01-01

    通过分析促食欲素A(Orexin A)在牦牛和黄牛消化道不同部位的分布特征及表达差异,探讨Orexin A对牦牛能量代谢的调节作用.运用免疫组织化学SP法检测Orexin A在牦牛和黄牛胃肠道的表达,利用Image‐ProPlus 6.0软件对其表达强度进行半定量分析.结果表明, Orexin A在牦牛和黄牛的皱胃、幽门、贲门和十二指肠均有表达,但表达特征不同;牦牛胃和十二指肠中Orexin A阳性反应产物极显著地低于黄牛(P<0.01);牦牛胃肠道中Orexin A阳性反应产物的吸光度分布为:皱胃区>幽门区>贲门区>十二指肠区(P<0.01).推测牦牛通过胃肠道Orexin A的低表达,调节中枢及外周组织的激素水平,从而维持机体能量代谢水平,适应青藏高原的特殊环境.%To explore the regulation effect of Orexin A on energy metabolism of yak by analyzing the difference of distribution and expression in gastrointestinal tract between yak and cattle . Immunohistochemistry is used to localize the expression of Orexin A in the gastrointestinal tract of the yak ,Image‐ProPlus 6.0 software is used to semi‐quantitative analyse the intensity of expression of Orexin A . Results show that Orexin A is widely expressed in the abomasum ,pylorus and duodenum cardia in yak and cattle ,which have different expressional characteristics ;The mean absorbance of Orexin A positive reaction product in stomach tissue and duodenum of yak is significantly lower than that of cattle ( P<0.01);By comparing the mean absorbance of Orexin A positive reaction product in the yak , it is found that its expression from strong to weak successively is abomasums area , pyloric region , cardiac region , and then duodenum area (P<0.01) .It indicats that the lower expression of Orexin A could regulate the hormone levels in the central and peripheral tissues to maintain its energy balance to adapt to the extreme environment of the Tibetan

  14. Whole-body valine and cysteine kinetics and tissue fractional protein synthesis rates in lambs fed Sulla (Hedysarum coronarium) and infected or not infected with adult Trichostrongylus colubriformis.

    Science.gov (United States)

    Bermingham, Emma N; McNabb, Warren C; Sutherland, Ian A; Sinclair, Bruce R; Treloar, Bryan P; Roy, Nicole C

    2006-07-01

    Poor growth during parasitic infection may be due to a redistribution of amino acids away from skeletal muscle protein synthesis to the intestinal site of infection. The effect of a Trichostrongylus colubriformis infection on whole-body amino acid kinetics and tissue fractional protein synthesis rates were determined in lambs fed fresh Sulla (Hedysarum coronarium; 800 g DM/d). Lambs were dosed with 6000 L3 Trichostrongylus colubriformis larvae daily for 6 d (n 6) or kept as parasite-free controls (n 6). On day 45 post-infection, the lambs received an intravenous injection of 2H2O and infusions (8 h) of [35S]sulphate to measure the size of the whole-body water and sulphate pools, respectively. On day 48, the lambs were continuously infused for 8 h with [3,4-3H]valine into the jugular vein as well as with [1-13C]valine and [35S]cysteine into the abomasum. After the 8 h infusions, the lambs were killed and tissue samples collected from the duodenum, ileum, mesenteric lymph nodes, liver, spleen, thymus, muscle and skin. Feed intake (769 v. 689 (sd 47) g DM/d) was not affected by infection, whereas liveweight gains (50 v. -50 (sd 70) g/d) were lower and intestinal worm burdens (240 v. 18,000 (sd 7000) worms) higher in the infected lambs. Parasitic infection increased the fractional protein synthesis rates in the small intestine, mesenteric lymph nodes and liver but did not affect skin and skeletal muscle fractional protein synthesis rates during the established parasitic infection.

  15. Severe dental fluorosis in juvenile deer linked to a recent volcanic eruption in Patagonia.

    Science.gov (United States)

    Flueck, Werner T; Smith-Flueck, Jo Anne M

    2013-04-01

    The Puyehue-Cordon Caulle volcanic eruption deposited large amounts of tephra (ashes) on about 36 million ha of Argentina in June of 2011. Tephra was considered chemically innoxious based on water leachates, surface water fluoride levels were determined to be safe, and livestock losses were attributable to inanition and excessive tooth wear. To evaluate effects on wild ungulates, we sampled wild red deer (Cervus elaphus) at 100 km from the volcano in September-November 2012. We show that tephra caused severe dental fluorosis, with bone fluoride levels up to 5,175 ppm. Among subadults, tephra caused pathologic development of newly emerging teeth typical of fluorosis, including enamel hypoplasia, breakages, pitting, mottling, and extremely rapid ablation of entire crowns down to underlying pulp cavities. The loss of teeth functionality affected physical condition, and none of the subadults was able to conceive. Susceptibility to fluorosis among these herbivores likely resides in ruminant food processing: 1) mastication and tephra size reduction, 2) thorough and repeated mixing with alkaline saliva, 3) water-soluble extraction in the rumen, and 4) extraction in the acidic abomasum. Although initial analyses of water and tephra were interpreted not to present a concern, ruminants as a major component of this ecosystem are shown to be highly susceptible to fluorosis, with average bone level increasing over 38-fold during the first 15.5 mo of exposure to tephra. This is the first report of fluorosis in wild ungulates from a volcanic eruption. The described impact will reverberate through several aspects of the ecology of the deer, including effects on population dynamics, morbidity, predation susceptibility, and other components of the ecosystem such as scavenger and plant communities. We anticipate further impact on livestock production systems, yet until now, existence of fluorosis had not been recognized.

  16. Survival and infectivity studies of in-vitro cultivated larvae of Haemonchus contortus in Sheep and Goats in Nigeria

    Directory of Open Access Journals (Sweden)

    A O Sonibare

    Full Text Available The survival and infectivity of indigenous isolates of Haemonchus contortus and in vitro cultivated infective larvae (L3 were studied. Active motile gravid females, adult and immature parasites were morphologically and morphometrically characterized and isolated from Haemonchus infected goats. These were subsequently inoculated into the abomasum of parasite free lambs through left sided laparatomy for production of fertile eggs to be used for coproculture. Harvested faeces from these lambs were cultured in a nutritive medium and kept in the incubator at 320C and room temperature 24-280C to obtain in vitro cultivated L of H. contortus. The infectivity and survival of in vitro cultivated L3 stored in refrigerator at 4-80C and under room temperature 24-280C were evaluated. Incubation period of 3 coproculture was observed to be shorter in medium at 320C than under room temperature 24-280C. The storage of in vitro cultivated L under different temperature range showed that the quantity of active larvae in a given volume 3 decreased at p<0.05 from the 5th day under 24-280C while at 4-80C became significantly lower from 20th day. Infectivity of isolate kept at 4-80C was sustained for 18-20 days while at 24-280C it was maintained for 10-12 days. Laboratory invitro cultivated L of H. contortus had limited infectivity and larvae mortality increase with time under different 3 temperatures. [Vet. World 2011; 4(12.000: 533-536

  17. Desenvolvimento alométrico do trato gastrintestinal de bezerros da raça holandesa alimentados com diferentes dietas líquidas durante o aleitamento - DOI: 10.4025/actascianimsci.v26i4.1729 Alometric growth of gastrointestinal tract of holstein calves fed with diferent liquid diets during milking period - DOI: 10.4025/actascianimsci.v26i4.1729

    Directory of Open Access Journals (Sweden)

    Silvana Martines Baraldi Artoni

    2004-04-01

    Full Text Available Neste trabalho, foram utilizados 12 bezerros da raça Holandesa distribuídos em três tratamentos, em delineamento inteiramente casualisado com quatro repetições. Os tratamentos leite integral e dois tipos de sucedâneos comerciais Lactal® e Destetor®, avaliaram o desenvolvimento alométrico do sistema digestório, altura da papila do rúmen e do retículo, e altura e comprimento das cristas do omaso. O aleitamento dos animais transcorreu por um período de 60 dias, e, ao serem abatidos, todos os órgãos do trato digestório foram pesados e comparados, com destaque para o estômago (rúmen, retículo, omaso e abomaso. Não foram observadas diferenças (p>0,05 nas proporções dos diferentes compartimentos do estômago dos bezerros submetidos aos tratamentos. No entanto, verificou-se diferença (pIn this work 12 Holstein calves were used distributed in three treatment, on a completely randomized desing with four replicatin. The treatments were used, integral milk and two types of comercial milk replacer Lactal® and Destetor® to evaluate the digestive system alometric growthof the including, rumen and reticulum papillae heigth and omasum rigde growth. The animals were milking for 60 days and after slaughtring, their digest system organs were weighed and stomach complex compared (rumen, reticulum, omasum and abomasum. No difference (P>0.05 due to treatments were obseved for the proportions among the diferent stomach compartiments of the calves. However, differences (P<0.05 for the papillae height of rumen and for the rigde lenght of omasum were observed. The milk replacers in this work resulted in a good calve growth being an alternative source of feed during mlking period.

  18. Efficacy of copper oxide wire particles against gastrointestinal nematodes in sheep and goats.

    Science.gov (United States)

    Soli, F; Terrill, T H; Shaik, S A; Getz, W R; Miller, J E; Vanguru, M; Burke, J M

    2010-02-26

    Profitable sheep and goat production in the USA is severely limited by gastrointestinal nematode (GIN) parasitism, particularly by Haemonchus contortus. Copper oxide wire particles (COWP) have anti-parasitic properties in the diet of small ruminants, but efficacy of COWP may differ between sheep and goats. In a study with weaned kids (Kiko x Spanish cross, 6 months old) and lambs (Katahdin or Dorper x Blackface crosses, 5 months old), grazing the same pasture area in Central Georgia, 2g of COWP in a gel capsule was given to half the animals of each species, while the other half were given no COWP. Fecal and blood samples were taken weekly to determine GIN fecal egg counts (FEC) and blood packed cell volume (PCV). After COWP treatment, animals were grazed for 4 weeks and then slaughtered, with adult GIN recovered from the abomasum and small intestines for counting and identification to species. For both sheep and goats, COWP treatment reduced EPG (P<0.05), increased PCV (P<0.05), and lowered abomasal GIN numbers (P<0.05). For EPG, these differences were 82.5 and 90.5% for sheep and goats, respectively, 26 days after treatment, while adult H. contortus were 67.2 and 85.8% lower for COWP-treated sheep and goats, respectively. In this study, COWP treatment was equally effective against H. contortus infection in lambs and kids and appears to be an effective method of controlling H. contortus infection for up to 6 weeks in small ruminants following weaning.

  19. Effects of wheat protein in milk replacers on abomasal emptying rate in calves.

    Science.gov (United States)

    Wittek, T; Ernstberger, M; Muckenhuber, M; Flöck, M

    2016-04-01

    Diarrhoea is a condition with tremendous impact on calf health. Infectious agents play a dominant role; however, non-infective factors may also contribute to pathogenesis of diarrhoea. One factor, the abomasal emptying rate, is mainly influenced by the composition of feed. The aim of the study was to assess the influence of different protein sources in milk replacers on abomasal emptying rate and clinical parameters. The effect of increasing age of the calves on abomasal emptying was also evaluated. The study compared abomasal emptying rates and clinical parameters in calves, which were fed either milk replacer containing only whey protein or one which partially contained wheat protein. Abomasal emptying rate was estimated by ultrasonography. Ten calves were used in the study over 18 days, and each calf was fed 3 periods of 3 days length using different milk replacers in an alternating crossover design. The abomasum was emptied significantly faster when the wheat protein containing milk replacer was fed (half-emptying time wheat protein 49.1 ± 4.1 min, half-emptying time milk protein 59.1 ± 7.4 min); however, clinical parameters and weight gain did not differ between the feeding regimes. Age did not significantly influence abomasal emptying rate. As milk replacers containing wheat proteins increased abomasal emptying rate, they may have a higher potential to initiate diarrhoea, especially if high volumes are fed. Thus, the feeding regimes are likely to be even more important when such milk replacers are used.

  20. Integrated assessment of ivermectin pharmacokinetics, efficacy against resistant Haemonchus contortus and P-glycoprotein expression in lambs treated at three different dosage levels.

    Science.gov (United States)

    Alvarez, Luis; Suarez, Gonzalo; Ceballos, Laura; Moreno, Laura; Canton, Candela; Lifschitz, Adrián; Maté, Laura; Ballent, Mariana; Virkel, Guillermo; Lanusse, Carlos

    2015-05-30

    The main goals of the current work were: (a) to assess the ivermectin (IVM) systemic exposure and plasma disposition kinetics after its administration at the recommended dose, x5 and x10 doses to lambs, (b) to compare the clinical efficacy of the same IVM dosages in lambs infected with an IVM-resistant isolate of Haemonchus contortus, and (c) to assess the expression of the transporter protein P-glycoprotein (P-gp) in H. contortus recovered at 14 days after administration of the IVM dose regimens. There were two separated trials where IVM was administered either subcutaneously (SC, Experiment I) or intraruminally (IR, Experiment II). Each experiment involved twenty-four (24) lambs artificially infected with a highly resistant H. contortus isolate. Animals were allocated into 4 groups (n=6) and treated with IVM at either 0.2 (IVM x1), 1 (IVM x5) or 2mg/kg (IVM x10). Plasma samples were collected up to 12 days post-treatment and analysed by HPLC. An untreated-control Group was included to assess the comparative anthelmintic efficacy of the different treatments. The level of expression of Pgp in H. contortus specimens obtained from lambs both untreated and IR treated with the different IVM doses was quantified by real time PCR. Parametric and non-parametric tests were used to compare the statistical significance of the results (PP-gp in adult H. contortus at 14 days post-treatment compared to samples collected from the untreated control group. An enhanced parasite exposure of the drug at the abomasum may explain the improved efficacy against this recalcitrant H. contortus isolate observed only after the IR administration at 5- and 10-fold the IVM therapeutic dosage.

  1. Aislamiento de Clostridium perfringens tipo D en un ternero lactante afectado con abomasitis ulcerativa Isolation of Clostridium perfringens type D from a suckling calve with ulcerative abomasitis

    Directory of Open Access Journals (Sweden)

    R. A ASSIS

    2002-01-01

    Full Text Available Se describe un brote de abomasitis ulcerativa asociada con infección por Clostridium perfringens tipo D en terneros lactantes. Seis terneros Holstein, de 2 semanas de edad, murieron después de presentar anorexia y apatía. Otros animales del mismo establecimiento habían muerto de la misma forma seis meses antes. A la necropsia el abomaso estaba muy distendido con contenido fluido y oscuro, la mucosa estaba edematosa y presentaba gran cantidad de úlceras milimétricas y habían depósitos de fibrina en la serosa. En el ciego de un animal se observaron extensas areas de infarto. En frotis de la mucosa abomasal se observaron bacilos cortos Gram positivos, no esporulados, aislándose de ella un cultivo rico de C. perfringens tipo D. Es probable que la baja inmunidad de los terneros debido a falta de calostro y estrés alimenticio, haya sido el predisponente para la infección por C. perfringens tipo DAn outbreak of ulcerative abomasitis in suckling calves associated with Clostridium perfrigens type D infection is described. Six twoweek old Holstein calves died after showing loss of appetite and lethargy. Other animals had died in similar circumstances during the previous six months. At necropsy, the abomasum was severely distended with dark fluid and the mucosa was oedematous and covered with many millimetric ulcers, while the serous surface of this organ was covered with fibrin. Several irregular black areas of infarcts were observed in the cecum of one animal. Histologically, the abomasal mucosa showed ulcers and haemorrhage, while the submucosa showed severe oedema and infiltration of neutrophils, lymphocytes and plasma cells. Short, thick, nonsporulated Gram positive rods were observed on smears of abomasal mucosa. C. perfringens type D was isolated from the abomasal mucosa. Low immunity and stress could have contributed to the pathogenesis of the lesions described

  2. Genetic parameters for producer-recorded health data in Canadian Holstein cattle.

    Science.gov (United States)

    Neuenschwander, T F-O; Miglior, F; Jamrozik, J; Berke, O; Kelton, D F; Schaeffer, L R

    2012-04-01

    Health traits are of paramount importance for economic dairy production. Improvement in liability to diseases has been made with better management practices, but genetic aspects of health traits have received less attention. Dairy producers in Canada have been recording eight health traits (mastitis (MAST), lameness (LAME), cystic ovarian disease (COD), left displaced abomasum (LDA), ketosis (KET), metritis (MET), milk fever (MF) and retained placenta (RP)) since April 2007. Genetic analyses of these traits were carried out in this study for the Holstein breed. Edits on herd distributions of recorded diseases were applied to the data to ensure a sufficient quality of recording. Traits were analysed either individually (MAST, LAME, COD) or were grouped according to biological similarities (LDA and KET, and MET, MF and RP) and analysed with multiple-trait models. Data included 46 104 cases of any of the above diseases. Incidence ranged from 2.3% for MF to 9.7% for MAST. MET and KET also had an incidence below 4.0%. Variance components were estimated using four different sire threshold models. The differences between models resulted from the inclusion of days at risk (DAR) and a cow effect, in addition to herd, parity and sire effects. Models were compared using mean squared error statistic. Mean squared error favoured, in general, the sire and cow within sire model with regression on DAR included. Heritabilities on the liability scale were between 0.02 (MET) and 0.21 (LDA). There was a moderate, positive genetic correlation between LDA and KET (0.58), and between MET and RP (0.79).

  3. Use of rumination and activity monitoring for the identification of dairy cows with health disorders: Part I. Metabolic and digestive disorders.

    Science.gov (United States)

    Stangaferro, M L; Wijma, R; Caixeta, L S; Al-Abri, M A; Giordano, J O

    2016-09-01

    The objectives of this study were to evaluate (1) the performance of an automated health-monitoring system (AHMS) to identify cows with metabolic and digestive disorders-including displaced abomasum, ketosis, and indigestion-based on an alert system (health index score, HIS) that combines rumination time and physical activity; (2) the number of days between the first HIS alert and clinical diagnosis (CD) of the disorders by farm personnel; and (3) the daily rumination time, physical activity, and HIS patterns around CD. Holstein cattle (n=1,121; 451 nulliparous and 670 multiparous) were fitted with a neck-mounted electronic rumination and activity monitoring tag (HR Tags, SCR Dairy, Netanya, Israel) from at least -21 to 80 d in milk (DIM). Raw data collected in 2-h periods were summarized per 24 h as daily rumination and activity. A HIS (0 to 100 arbitrary units) was calculated daily for individual cows with an algorithm that used rumination and activity. A positive HIS outcome was defined as a HIS of metabolic and digestive disorders combined (n=104). Days (mean and 95% CI) from the first positive HIS metabolic and digestive disorders, respectively. The patterns of rumination, activity, and HIS for cows flagged by the AHMS were characterized by lower levels than for cows without a health disorder and cows not flagged by the AHMS from -5 to 5 d after CD, depending on the disorder and parameter. Differences between cows without health disorders and those flagged by the AHMS for blood markers of metabolic and health status confirmed the observations of the CD and AHMS alerts. The overall sensitivity and timing of the AHMS alerts for cows with metabolic and digestive disorders indicated that AHMS that combine rumination and activity could be a useful tool for identifying cows with metabolic and digestive disorders.

  4. Natural Outbreak of BVDV-1d-Induced Mucosal Disease Lacking Intestinal Lesions.

    Science.gov (United States)

    Bianchi, M V; Konradt, G; de Souza, S O; Bassuino, D M; Silveira, S; Mósena, A C S; Canal, C W; Pavarini, S P; Driemeier, D

    2017-03-01

    Bovine viral diarrhea virus (BVDV) belongs to the Pestivirus genus, which is further divided into subgenotypes (1a-1u and 2a-c). When persistent infection occurs, the calf will be immunotolerant to BVDV and possibly develop mucosal disease. This study describes an outbreak of BVDV-1d-induced mucosal disease lacking intestinal lesions. Eleven calves presented with anorexia, sialorrhea, lameness, recumbency, and death. Three calves were necropsied, showing ulceration of the interdigital skin and the oral and nasal mucosa; linear ulcers in the tongue, esophagus, and rumen; and rounded ulcers in the abomasum. Microscopically, mucosa and skin had superficial necrosis, with single-cell necrosis and vacuolation in epithelial cells, and severe parakeratosis. Immunohistochemistry (IHC) showed BVDV antigen in the cytoplasm of epithelial cells in skin and mucosa. All 11 dead calves were positive upon reverse transcription-polymerase chain reaction (RT-PCR) for the detection of Pestivirus along with another 11 live calves from the herd, which were positive again by RT-PCR and IHC after a 4-week interval. Sequencing of the 5' untranslated region and N-terminal protease showed that viruses from these 22 calves were homologous and of subgenotype BVDV-1d. Cytopathic BVDV was isolated from 8 of 11 dead calves, but only noncytopathic BVDV was isolated from the 11 live animals. The findings indicate that this was an outbreak of mucosal disease caused by BVDV-1d, with high morbidity, and lesions restricted to the upper alimentary system and skin and absent from intestine. Thus, the epidemiological and pathological features in this form of mucosal disease may be similar to vesicular diseases, including foot and mouth disease.

  5. Effect of Calcium Borogluconate Injection pre and Immediately Postpartum on Production Performance, Incidence of Metabolic Disorders and Situation of the Uterus After Calving in Cows Fed Anionic Diets

    Directory of Open Access Journals (Sweden)

    H. Amanlou

    2012-10-01

    Full Text Available The objective of this study was to determine the effect of calcium borogluconate injection 48 and 24 hours prepartum and immediately after parturition on dry matter intake at day of calving, milk yield, incidence of metabolic disorders and uterine conditions during the first 21 days postpartum in cows fed anionic diets. Thirty six lactating dairy cows were balanced by parity (1, 2, 3+ and allocated to 4 subcutaneous injection treatments of 15.2 gr calcium as borogluconate at different times pre and postpartum than expected calving date . Group 1 consisted of 9 cows receiving no treatment before or after parturition. Group 2 consisted of 9 cows receiving calcium borogluconate 48 h prepartum.Group 3 consisted of 9 cows receiving calcium borogluconate 24 h prepartum. Group 4 consisted of 9 cows receiving 15.2 gr of ca as borogluconate immediately postpartum. Dry matter intake was in treatments 1, 2, 3 and 4 was 10.87, 12.32, 12.63 and 15.03 kg in the first 24 h after calving, respectively And indicated significantly difference among treatments. milk yield in 2, 3 and 4 treatments was significantly greater than group 1 in the first 21 days of lactation (38.7, 38.9, 40.3 vs 36.07 kg/d. Treatment had a significant effect on the subclinical hypocalcemia incidence so incidence risk of subclinical hypocalcemia in 1 treatment was 10 times more likely than 4 treatment. Data regression analysis indicated that 4 treatment had a significant effect on the ketosis incidence. So that the cows no calcium injection 2.43 times more likely to experience ketosis than 4 treatment. But experimental treatments were not significantly effect on the incidence of milk fever, displacement abomasum, retained placenta, metritis and endometritis. In general, calcium injection, especially immediately after parturation increased the dry matter intake in day of calving and reduced metabolic disorders such as hypocalcaemia and ketosis.

  6. 饲草中缩合单宁的研究进展%Progress in Condensed Tannins of Forage

    Institute of Scientific and Technical Information of China (English)

    朱琳; 陈鑫珠; 张建国

    2012-01-01

    Feeding a high concentration of condensed tannins will reduce protein degradation and voluntary feed intake, damage abomasum or intestine. However, appropriate concentration of condensed tannins have beneficial effects on prevention of bloat and pulmonary edema, reducing parasitic disease and pastoral flavour in meat products, improving animal performance. Condensed tannins are clearly a "double edged sword" for feeding value. Successful management of condensed tannins depends on a combination of chemical analysis and animal experimentation. This paper is a review on nutrition and anti-nutrition of condensed tannins for ruminants, the factors affecting content of condensed tannins in forage, and the methods of controling concentration of condensed tannins. Research on successful management of condensed tannins will offer a real benefit for farmers.%饲料中高浓度的缩合单宁会降低动物对营养物质的消化利用率,影响自愿采食量,甚至损伤肠胃.适宜含量的缩合单宁,又能阻止臌胀病、肺水肿的发生,降低寄生虫病害的影响,改善肉质品味,提高动物的生产性能.论文概述了缩合单宁的营养作用、抗营养作用,影响饲草缩合单宁含量的因素,以及控制缩合单宁含量的方法,以便在实践中合理应用缩合单宁.

  7. An attempt to prevent production diseases in dairy cows by intense monitoring and ad hoc treatment

    Directory of Open Access Journals (Sweden)

    Matteo G. Coiatelli

    2015-11-01

    Full Text Available A trial has been performed on 201 dairy cows from two Italian commercial herds in order to verify whether the mitigation of a recognized negative energy balance (NEB by a therapeutic mean may influence the incidence of peri-partum diseases. All animals were tested for beta-hydroxybutyrate (β-HOB and non-esterified fatty acids (NEFA three times a week from 2 weeks before the expected due time to 2 weeks after calving. Animals whose blood levels were above β-HOB>1.2 or NEFA>0.5 mmol/L were declared POSITIVE and then split in two groups. Group T animals (n=57 were treated with a glycogenic treatment (ENERGAN KETOSIS, Virbac. The treatment was repeated daily as long as biochemical values remained abnormal. Group C animals (n=48 served as untreated controls. Animals with values within the physiological range over the study period were said NEGATIVE (n=96. This study confirmed that animals presenting excessive β-HOB or NEFA concentrations show a higher risk to get sick during the study period (P<0.05, the major risk being clinical ketosis (P<0.01 and in a lesser extend retention of the placenta (P=0.09. The application of a glycogenic treatment did not show an impact on blood metabolite levels due to huge individual differences. However, application of the treatment for an average duration of 5 days tends to reduce the incidence of all the diseases related to a NEB. Moreover, untreated control animals were more likely to get dislocation of the abomasum (P<0.05 than NEGATIVE animals whereas treated animals were not.

  8. A Study of Eimeria Species in Sheep in Mosul City

    Directory of Open Access Journals (Sweden)

    M. H. Hasan

    2012-01-01

    Full Text Available The study was conducted to diagnose and study species of Eimeria in sheep in Mosul city from beginning of September2009 to end May 2010, as well as to determine the percentage and intensity of infection of Eimeria species. Five hundredfecal samples of sheep with different ages were collected from different areas of the Mosul city. The results showed that totalpercentage of Emeria infection was 63.6%. The variations in percentage of infection were recorded according to month ofstudy. Highest percentage was recorded in March being 89.2% and the lowest in September 25.9%. The species E. ovinarecorded the highest infection rate 86.7%, while the species E. granulosa represented lowest infection rate 10%. Moreover theintensity of infection was higher in young ages and lower in adult. The results were detected that indoor sheep infection withhigh parasitic infection 69.9% whereas outdoor animals have an infection rate 25.3%. The morphological characters of oocystswere varied according to species of Eimeria has been studied. Fifty of intestinal and abomasal samples from both slaughteredin shops butchery in Mosul city and dead animals were examined to detect Eimeria infection, and results show that infectionpercentage was 56.4% in intestine of slaughtered animals and 36.3% in dead animal. Moreover no infection of Eimeria weredetected in abomasums in both slaughtered and dead animals. The oocysts of (E. parva, E.pallida and E. ovinoidalis detectedat more than 5000 oocysts per gram of intestinal contents. The intestinal secraping stained with Giemsa stain reveals thepresence of different developmental stages of parasites in wall of intestine. The histopathological sections of intestine revealedthe different pathological changes concerning of Eimeria infection.

  9. A biometric study of the digestive tract of one-humped camel (camelus dromedarius fetuses

    Directory of Open Access Journals (Sweden)

    A. Bello

    2012-09-01

    Full Text Available A Biometrical study was conducted on the digestive tract of 35 foetuses of the one-humped camel collected from the Sokoto metropolitan abattoir, over a period of five months at different gestational ages. The approximate age of the foetuses was estimated from the crown vertebral rump length (CVRL and samples were categorised into first, second and third trimester. The mean body weight of the foetus at first, second third trimester ranged from 1.40 ± 0.06 kg, 6.10 ± 0.05 kg and 17.87 ± 0.6 kg respectively. The mean weights of the entire digestive system at first, second and third trimester were 0.80 ± 0.07 kg, 2.13 ± 0.04 kg and 4.86 ± 0.08 kg respectively. The mean weights of the digestive tract at first, second and third trimester were 0.53 ± 0.07 kg, 1.03 ± 0.05 and 2.43 ± 0.07 kg respectively. The small intestine at first trimester were found not to have any clear demarcation to show duodenum, jejunum and ileum; the entire small intestine was found to be 76.00 ± 3.00 cm at first trimester and showed clear demarcation at second and third trimesters.. The mean volumes of the entire stomach (rumen, reticular and abomasum ranged from 136.67 ± 8.30 cm3 at first trimester to 353.33 ± 6.50 cm3 at third trimester. It was observed that there was increase in body weight, organ weight and individual segment of the digestive tract of the fetuses with advancement in gestation period. A geometrical increase in length and diameter of the various segments of the digestive tract showed a significant difference (P≤ 0.05 with advancement in gestational period.

  10. Managing anthelmintic resistance in small ruminant livestock of resource-poor farmers in South Africa : review article

    Directory of Open Access Journals (Sweden)

    A.F. Vatta

    2006-06-01

    Full Text Available Gastrointestinal parasitism is one of the most important disease complexes of sheep and goats impacting on the resource-poor livestock farmer. Of the responsible nematodes, Haemonchus contortus, a blood-sucking worm of the abomasum, poses possibly the greatest threat. Over the past several decades, the worm has been controlled through the use of anthelmintics, but the emergence of anthelmintic resistance has threatened this chemotherapeutic approach. In Africa, the overall prevalence of anthelmintic resistance has not been extensively investigated, particularly within the resource-poor farming sector, but resistance has been reported from at least 14 countries with most of the reports emanating from Kenya and South Africa and the majority concerning H. contortus. While levels of resistance under commercial sheep farming systems in South Africa is considered to be amongst the worst in the world, resistance has also been reported from the resource-poor farming sector. Increases in productivity and reproduction of livestock and the development of markets for sale of animals are seen by international funding bodies as a way out of poverty for communities that keep livestock. This must lead to the greater need for parasite control. At such times, the risk of levels of anthelmintic resistance escalating is much greater and there is therefore a need to look at alternatives to their use. Proposed strategies include the appropriate, but judicious use of anthelmintics by application of the FAMACHA(c system and the use of alternatives to anthelmintics such as strategic nutrient supplementation. It is also very clear that there is a strong demand for knowledge about animal diseases, including helminthosis, and their effective management in the resource-poor livestock farming communities. This is an important challenge to meet.

  11. Detection of PrP(Sc) in peripheral tissues of clinically affected cattle after oral challenge with bovine spongiform encephalopathy.

    Science.gov (United States)

    Franz, Martin; Eiden, Martin; Balkema-Buschmann, Anne; Greenlee, Justin; Schatzl, Hermann; Fast, Christine; Richt, Jürgen; Hildebrandt, Jan-Peter; Groschup, Martin H

    2012-12-01

    Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative prion disease that mainly affects cattle. Transmission of BSE to humans caused a variant form of Creutzfeldt-Jakob disease. Following infection, the protease-resistant, disease-associated isoform of prion protein (PrP(Sc)) accumulates in the central nervous system and in other tissues. Many countries have defined bovine tissues that may contain prions as specified risk materials, which must not enter the human or animal food chains and therefore must be discarded. Ultrasensitive techniques such as protein misfolding cyclic amplification (PMCA) have been developed to detect PrP(Sc) when present in minuscule amounts that are not readily detected by other diagnostic methods such as immunohistochemistry or Western blotting. This study was conducted to determine when and where PrP(Sc) can be found by PMCA in cattle orally challenged with BSE. A total of 48 different tissue samples from four cattle infected orally with BSE at various clinical stages of disease were examined using a standardized PMCA protocol. The protocol used brain homogenate from bovine PrP transgenic mice (Tgbov XV) as substrate and three consecutive rounds of PMCA. Using this protocol, PrP(Sc) was found in the brain, spinal cord, nerve ganglia, optic nerve and Peyer's patches. The presence of PrP(Sc) was confirmed in adrenal glands, as well as in mesenteric lymph nodes - a finding that was reported recently by another group. Interestingly, additional positive results were obtained for the first time in the oesophagus, abomasum, rumen and rectum of clinically affected cattle.

  12. Molecular and morphological characterization of Parabronema skrjabini of sheep and goats at three different geographical zones in Iran.

    Science.gov (United States)

    Hasheminasab, Seyed Sajjad; Jalousian, Fatemeh; Meshgi, Behnam

    2016-01-01

    Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular and morphological aspects of Parabronema skrjabini in sheep and goats in Iran. The worms were collected from these animal species from three different regions. An internal transcribed spacer 2 ribosomal DNA (ITS2-rDNA) fragment of Parabronema skrjabini was amplified by polymerase chain reaction (PCR) using a pair of specific primers (Para-Ir-R and Para-Ir-F). Morphological studies based on the body length, the frontal shield, spicules of male and egg dimensions were performed. ITS2-rDNA sequences were between 167 and 299 bp in different isolates. ITS2 homology in different isolates was between 68% and 77% compared with the sequence data in GenBank. Morphological results showed that the average length of male and female worms in sheep were 16.5 mm and 36 mm and in goats 16 mm and 35.5 mm, respectively. The average length of the small and large spicules in sheep were 657.5 μm and 304.07 μm and in goats 653.08 μm and 302.66 μm, respectively. To our knowledge, this is the first study in the world exploring the genetic diversity of Parabronema in sheep and goats. Add this sentence in discussion: the low ITS2-rDNA identity in different isolates from Iran as compared to the reference sequence in GenBank (68-77%) raise questions regarding the species identity of the parasites isolated in Iran.

  13. Diagnosis, Treatment and Management of Haemonchus contortus in Small Ruminants.

    Science.gov (United States)

    Besier, R B; Kahn, L P; Sargison, N D; Van Wyk, J A

    2016-01-01

    Haemonchus contortus is a highly pathogenic, blood-feeding nematode of small ruminants, and a significant cause of mortalities worldwide. Haemonchosis is a particularly significant threat in tropical, subtropical and warm temperate regions, where warm and moist conditions favour the free-living stages, but periodic outbreaks occur more widely during periods of transient environmental favourability. The clinical diagnosis of haemonchosis is based mostly on the detection of anaemia in association with a characteristic epidemiological picture, and confirmed at postmortem by the finding of large numbers of H. contortus in the abomasum. The detection of impending haemonchosis relies chiefly on periodic monitoring for anaemia, including through the 'FAMACHA' conjunctival-colour index, or through faecal worm egg counts and other laboratory procedures. A range of anthelmintics for use against H. contortus is available, but in most endemic situations anthelmintic resistance significantly limits the available treatment options. Effective preventative programmes vary depending on environments and enterprise types, and according to the scale of the haemonchosis risk and the local epidemiology of infections, but should aim to prevent disease outbreaks while maintaining anthelmintic efficacy. Appropriate strategies include animal management programmes to avoid excessive H. contortus challenge, genetic and nutritional approaches to enhance resistance and resilience to infection, and the monitoring of H. contortus infection on an individual animal or flock basis. Specific strategies to manage anthelmintic resistance centre on the appropriate use of effective anthelmintics, and refugia-based treatment schedules. Alternative approaches, such as biological control, may also prove useful, and vaccination against H. contortus appears to have significant potential in control programmes.

  14. A macro- and light microscopical study of the pathology of Calicophoron microbothrium infection in experimentally infected cattle

    Directory of Open Access Journals (Sweden)

    M. Mavenyengwa

    2005-09-01

    Full Text Available Twelve Tuli weaner steers aged 1 year were randomly subdivided into three groups of four animals and infected with different doses of Calicophoron microbothrium metacercariae. Each animal in Group I received a low dose (LD of 5 000 metacercariae, Group II a medium dose (MD of 15 000 metacercariae, Group III a high dose (HD of 25 000 metacercariae and one additional animal was kept as an uninfected control (C. After infection, one animal from each group was slaughtered on Day 28, 42, 56 and 84 post infection (pi and samples from the ileum, jejunum, duodenum, abomasum and the rumen were collected for histopathological and cytological examination. On Day 28 pi, the gross pathological lesions observed in the duodenum of the LD and the MD animals were similar and comprised duodenal thickening, corrugation, hyperaemia, petechiation and ulceration. In the HD animal the duodenal lesions were similar but more severe. The abomasal folds were severely oedematous in the MD group and nearly occluded the abomasal lumen. Moderate oedema of the abomasal folds was also present in the LD and HD animals. The gross pathological lesions regressed in all the infected groups with increasing age of infection and had disappeared completely by Day 56 pi. On Day 28 pi the histopathological lesions in the duodenum and jejunum of the LD and MD groups were similar, comprising subtotal villous atrophy, hyperplasia of Brunner's glands and Peyer's patches and moderate infiltration of eosinophils, mast cells and a few globule leukocytes, basophils and lymphocytes in the lamina propria. The HD group had total villous atrophy, severe hyperplasia and cystic dilatation of Brunner's glands, which had expanded to cover the entire submucosa. On Day 42 pi the histopathological lesions were still present in the MD and the HD groups comprising subtotal villous atrophy and hyperplasia of Brunner's glands. Heavy infiltrations of eosinophils, moderate amounts of mast cells and a few

  15. Digestive development in neonatal dairy calves with either whole or ground oats in the calf starter.

    Science.gov (United States)

    Suarez-Mena, F X; Heinrichs, A J; Jones, C M; Hill, T M; Quigley, J D

    2015-05-01

    A series of 3 trials was conducted to determine effects of whole or ground oats in starter grain on reticulorumen fermentation and digestive system development of preweaned calves. Male Holstein calves (43.1±2.3kg at birth; n=8, 9, and 7 for trials 1, 2, and 3, respectively) were housed in individual pens in a heated facility; bedding was covered with landscape fabric to prevent consumption of bedding by the calves. In trials 1 and 2 only, calves were fitted with rumen cannulas by wk 2 of life. In all trials, a fixed amount of starter (containing 25% oats either ground and in the pellet or whole) was offered daily; orts were fed through the cannula in trials 1 and 2. Calves were randomly assigned to an all-pelleted starter or pellets plus whole oats. Rumen contents (trials 1 and 2) were sampled weekly at -8, -4, 0, 2, 4, 8, and 12 h after grain feeding for determination of pH and volatile fatty acids. Calves were killed 3 wk (trial 1) or 4 wk (trials 2 and 3) after grain was offered; organs were harvested, emptied, rinsed, and weighed to gauge digestive organ development. Starter intake was not different between treatments. Weekly measurements of rumen digesta pH did not change and only subtle changes were observed in molar proportions of individual volatile fatty acids. Molar proportion of butyrate and pH linearly decreased with age, whereas acetate proportion increased. Reticulorumen weight and papillae length tended to be greater for calves fed pelleted starter, whereas abomasum weight was greater for calves fed pellets plus whole oats. Fecal particle size and starch content were greater for calves fed pellets plus whole oats. Under the conditions of this study, physical form of oats in starter grain did not affect rumen fermentation measurements; greater rumen weight and papillae length in calves fed pelleted starter may be the result of greater nutrient availability of ground oats. Under the conditions of this study with young calves on treatments for

  16. Sobre um surto de envenenamento por derivado cumarínico em bovinos About an outbreak of cumarin poisoning in cattle

    Directory of Open Access Journals (Sweden)

    Marilene F. Brito

    2005-09-01

    Full Text Available Relata-se um surto de envenenamento por derivado cumarínico em bovinos confinados, ocorrido no município de Seropédica, Rio de Janeiro, no qual morreram duas vacas e um touro, de um total de 43 animais. Entre as principais manifestações clínicas observaram-se apatia, anorexia, sialorréia, diarréia sanguinolenta, incoordenação e dificuldade respiratória. À necropsia observaram-se principalmente hemorragias de intensidade e formas diversas, em vários órgãos. O exame histopatológico não evidenciou alterações, além de hemorragias. A pesquisa toxicológica em conteúdos de rúmen, retículo, omaso e abomaso, em amostras de fígado e rim, bem como da cevada coletada no cocho, resultou positiva para o grupo de derivados cumarínicos que inclui warfarina, bromadiolone e bromadifacoum. Não se pode excluir uma ação intencional ou acidental, visto que os animais que não estavam se alimentando no mesmo cocho, não adoeceram.An outbreak of cumarin poisoning which occurred in feedlot cattle in the county of Seropédica, Rio de Janeiro, is described. Three from 43 animal died. The main manifestations included bloody diarrhea, apathy, anorexia, sialorrhoe, incoordenation and dyspnoe. At post-mortem examinations there were mainly hemorrhages of varies forms and intensity in several organs, which also were seen at histopathological examination. Analysis for cumarin derivates of rumen, reticulum, omasum and abomasum contents, of liver and kidney samples, as well as of food collected from the trough, resulted positive for cumarin compounds which included warfarin, bromadiolone and brodifacoum. Feeding experiments with food stored at the property resulted negative. An intentional or accidental act could not be excluded. Animals that did not feed from the same trough did not get sick.

  17. Discovery of quantitative trait loci for resistance to parasitic nematode infection in sheep: I. Analysis of outcross pedigrees

    Directory of Open Access Journals (Sweden)

    Greer Gordon J

    2006-07-01

    Full Text Available Abstract Background Currently most pastoral farmers rely on anthelmintic drenches to control gastrointestinal parasitic nematodes in sheep. Resistance to anthelmintics is rapidly increasing in nematode populations such that on some farms none of the drench families are now completely effective. It is well established that host resistance to nematode infection is a moderately heritable trait. This study was undertaken to identify regions of the genome, quantitative trait loci (QTL that contain genes affecting resistance to parasitic nematodes. Results Rams obtained from crossing nematode parasite resistant and susceptible selection lines were used to derive five large half-sib families comprising between 348 and 101 offspring per sire. Total offspring comprised 940 lambs. Extensive measurements for a range of parasite burden and immune function traits in all offspring allowed each lamb in each pedigree to be ranked for relative resistance to nematode parasites. Initially the 22 most resistant and 22 most susceptible progeny from each pedigree were used in a genome scan that used 203 microsatellite markers spread across all sheep autosomes. This study identified 9 chromosomes with regions showing sufficient linkage to warrant the genotyping of all offspring. After genotyping all offspring with markers covering Chromosomes 1, 3, 4, 5, 8, 12, 13, 22 and 23, the telomeric end of chromosome 8 was identified as having a significant QTL for parasite resistance as measured by the number of Trichostrongylus spp. adults in the abomasum and small intestine at the end of the second parasite challenge. Two further QTL for associated immune function traits of total serum IgE and T. colubiformis specific serum IgG, at the end of the second parasite challenge, were identified on chromosome 23. Conclusion Despite parasite resistance being a moderately heritable trait, this large study was able to identify only a single significant QTL associated with it. The QTL

  18. Association between body energy content in the dry period and post-calving production disease status in dairy cattle.

    Science.gov (United States)

    Smith, G L; Friggens, N C; Ashworth, C J; Chagunda, M G G

    2017-02-15

    The transition from gestation to lactation is marked by significant physiological changes for the individual cow such that disease incidence is highest in early lactation. Around the time of calving, cows rely on mobilisation of body energy reserves to fill the energy deficit created by an increase in nutrient demands at a time of restricted feed intake. It is well established that monitoring of body energy reserves in lactation is an important component of herd health management. However, despite their influence on future health and productivity, monitoring of body energy reserves in the dry period is often sparse. Further, there is increasing concern that current dry off management is inappropriate for modern cattle and may influence future disease risk. This study aimed to identify candidate indicators of early lactation production disease from body energy data collected in the dry period and production data recorded at the time of dry off. Retrospective analysis was performed on 482 cow-lactations collected from a long-term Holstein-Friesian genetic and management systems project, the Langhill herd in Scotland. Cow-lactations were assigned to one of four health groups based on health status in the first 30 days of lactation. These four groups were as follows: healthy, reproductive tract disorders (retained placenta and metritis), subclinical mastitis and metabolic disorders (ketosis, hypocalcaemia, hypomagnesaemia and left displaced abomasum). ANOVA, employing a GLM was used to determine effects for the candidate indicator traits. Cows which were diagnosed with a reproductive tract disorder in the first 30 days of lactation experienced a significantly greater loss in body energy content, body condition score and weight in the preceding dry period than healthy cows. The rate of change in body energy content during the first 15 days of the dry period was -18.26 MJ/day for cows which developed reproductive tract disorder compared with +0.63 MJ/day for healthy cows

  19. The effects of feeding sericea lespedeza hay on growth rate of goats naturally infected with gastrointestinal nematodes.

    Science.gov (United States)

    Moore, D A; Terrill, T H; Kouakou, B; Shaik, S A; Mosjidis, J A; Miller, J E; Vanguru, M; Kannan, G; Burke, J M

    2008-09-01

    Goat production is increasing in the United States due to high ethnic demand, but infection with gastrointestinal nematode (GIN) parasites is a major constraint to the industry. Increasing GIN resistance to chemical anthelmintics worldwide has led to the development of alternative control strategies, including use of forages containing condensed tannins (CT). An experiment was designed using infected and dewormed male kids (Kiko x Spanish, 6 mo old, 18.9 +/- 3.25 kg) fed diets containing 25% concentrate and either 75% sericea lespedeza [SL; Lespedeza cuneata (Dum-Cours.) G. Don], a high CT forage (87 to 181 g of CT/kg), or 75% bermudagrass [BG; Cynodon dactylon (L.) Pers.] hay (n = 10/treatment). The kids were weighed every 14 d, and fecal and blood samples were taken weekly for fecal egg counts and packed cell volume determination, respectively. Fecal cultures were processed every 14 d to determine CT effect on larval development. At slaughter, adult GIN were collected from the abomasum and small intestines for counting and speciation. Blood samples were also analyzed for plasma urea-N, and ruminal VFA and pH were determined. The infected SL-fed kids had consistently lower (P < 0.05) fecal egg counts than the infected BG goats throughout the trial and greater (P < 0.05) packed cell volume beginning by d 77. Average daily gain was greater (P < 0.001) in kids fed SL- than BG-based diets, regardless of infection status (104.3 +/- 5.0 and 75.5 +/- 4.8 g/d, respectively). Total VFA and acetate concentrations were greater (P < 0.001) in the BG- than in SL-fed goats, whereas propionate levels were unaffected by diet. Acetate:propionate ratio (P = 0.01) and plasma urea-N (P = 0.03) levels were greater in BG-fed goats, whereas rumen pH was greater (P < 0.001) in the SL-fed goats. Feeding SL hay can reduce GIN infection levels and increase performance of goats compared with BG hay.

  20. Efficacy of sericea lespedeza hay as a natural dewormer in goats: dose titration study.

    Science.gov (United States)

    Terrill, T H; Dykes, G S; Shaik, S A; Miller, J E; Kouakou, B; Kannan, G; Burke, J M; Mosjidis, J A

    2009-07-07

    Gastrointestinal nematodes (GIN) parasitism is the greatest threat to economic sheep and goat production in the southern USA, and there is widespread prevalence of GIN resistance to broad-spectrum anthelmintics in this region. A natural alternative for controlling GIN in small ruminants is feeding hay of sericea lespedeza [SL, Lespedeza cuneata (Dum.-Cours., G. Don)], a perennial warm-season legume high in condensed tannins. To determine the level of SL needed to reduce GIN infection, a confinement study was completed with 32 Spanish/Boer/Kiko cross yearling bucks offered one of four diets with 75% hay and 25% concentrate (n=8, 2 pens/treatment, 4 goats/pen). The hay portion of each diet consisted of a combination of ground SL (0%, 25%, 50%, and 75% of the diet) and bermudagrass [BG, Cynodon dactylon (L.) Pers.; 75%, 50%, 25%, and 0% of the diet]. The bucks were allowed to acquire a natural GIN infection on pasture prior to moving to the pens. After a 3-week adjustment period in the pens, the goats were stratified by fecal egg count (FEC) and packed cell volume (PCV), randomly assigned to treatments and pens, and then fed the treatment diets for six weeks. During the experimental period, fecal and blood samples were collected from individual animals weekly to determine FEC and PCV, respectively. Adult worms from abomasum and small intestines were collected for counting and identification of species at slaughter. Goats fed SL hay at 25%, 50%, and 75% of the diet had 45.3% (P=0.2048), 66.3% (P=0.0134), and 74.5% (P=0.0077) lower FEC than control animals (75% BG hay) after 21 days. The 50% and 75% SL goats had 84.6% (P=0.0625) and 91.9% (P=0.0340) lower FEC than controls by day 42. The 75% SL-fed goats tended to have higher (P=0.0624) PCV and had fewer (P=0.035) abomasal worms than control animals, while PCV and adult worm numbers of the 50% and 25% SL goats were not different from controls. The optimum level of SL hay in the diet for reducing worm numbers of small

  1. The effects of frequency of feeding on some quantitative aspects of digestion in the rumens of growing steers.

    Science.gov (United States)

    McAllan, A B; Lewis, P E; Griffith, E S

    1987-09-01

    Three steers with simple rumen and abomasal cannulas were given ground and pelleted diets containing predominantly dried grass meal (DG) or rolled barley (RB). Diets were given at frequencies of two or eight feeds/d in a simple changeover design. Chromic oxide and polyethylene glycol were given as flow markers and flows (g/24 h) of organic matter (OM), nitrogenous and carbohydrate compounds were calculated. Ribonucleic acid and 35S were used as microbial markers and diaminopimelic acid (DAP) as a bacterial marker. Frequency of feeding had no significant effect on mean rumen pH, ammonia levels or liquid outflow rates with either diet. Rumen volume was decreased and abomasal digesta flow increased on Diet DG with more feeds but these parameters were unaffected with Diet RB. Increased feeding frequency with both feeds resulted in increased numbers of protozoa. There were no significant effects of feeding frequency of Diet DG on the abomasal flows of any of the nitrogenous constituents measured. However, there was a significant increase in microbial-N flow from 33 to 43 g/d with more frequent feeding of diet RB which was not reflected in bacterial-N flow as measured by DAP. The apparent digestion of OM in the rumen, expressed as g/g intake with diet DG was 0.41 and 0.31 for two feeds and eight feeds/d respectively. Corresponding values for diet RB were 0.56 and 0.63 respectively. The reduction in OM digestion with frequent feeding of diet DG was reflected in similarly reduced rumen digestibilities of all dietary carbohydrate components whereas the increase in OM digestion with diet RB was reflected only by the component sugars of the dietary fibre. The efficiencies of microbial protein synthesis (expressed as gMN/kg ADOM) increased from 36 to 46 when the feeding frequency of diet DG was increased from two to eight times/d. No significant effect of frequency of feeding was found for diet RB. Mouth to abomasum degradation of feed-N (expressed as g/g intake) of 0.64 was

  2. Immunohistochemical evidence for an endocrine/paracrine role for ghrelin in the reproductive tissues of sheep

    Directory of Open Access Journals (Sweden)

    Brown Yvonne A

    2005-10-01

    Full Text Available Abstract Background The gut hormone, ghrelin, is involved in the neuroendocrine and metabolic responses to hunger. In monogastric species, circulating ghrelin levels show clear meal-related and body weight-related changes. The pattern of secretion and its role in ruminant species is less clear. Ghrelin acts via growth hormone secretagogue receptors (GHSR-1a to alter food intake, fat utilization, and cellular proliferation. There is also evidence that ghrelin is involved in reproductive function. In the present study we used immunohistochemistry to investigate the presence of ghrelin and GHSR-1a in sheep reproductive tissues. In addition, we examined whether ghrelin and GHSR-1a protein expression is developmentally regulated in the adult and fetal ovine testis, and whether there is an association with markers of cellular proliferation, i.e. stem cell factor (SCF and proliferating cell nuclear antigen (PCNA. Methods Antibodies raised against ghrelin and its functional receptor, GHSR-type 1a, were used in standard immunohistochemical protocols on various reproductive tissues collected from adult and fetal sheep. GHSR-1a mRNA presence was also confirmed by in situ hybridisation. SCF and PCNA immunoexpression was investigated in fetal testicular samples. Adult and fetal testicular immunostaining for ghrelin, GHSR-1a, SCF and PCNA was analysed using computer-aided image analysis. Image analysis data were subjected to one-way ANOVA, with differences in immunostaining between time-points determined by Fisher's least significant difference. Results In adult sheep tissue, ghrelin and GHSR-1a immunostaining was detected in the stomach (abomasum, anterior pituitary gland, testis, ovary, and hypothalamic and hindbrain regions of the brain. In the adult testis, there was a significant effect of season (photoperiod on the level of immunostaining for ghrelin (p Conclusion Evidence is presented for the presence of ghrelin and its receptor in various reproductive

  3. Functional properties of butter oil made from bovine milk with experimentally altered fat composition.

    Science.gov (United States)

    Ortiz-Gonzalez, G; Jimenez-Flores, R; Bremmer, D R; Clark, J H; DePeters, E J; Schmidt, S J; Drackley, J K

    2007-11-01

    Modification of milk fat composition might be desirable to alter manufacturing characteristics or produce low saturated fat dairy products that more closely meet consumer dietary preferences. The aim of this research was to evaluate functional properties of butter oil obtained from milks with fat composition modified by altering the profile of long-chain fatty acids (FA) absorbed from the small intestine of cows. A control and 5 mixtures of long-chain free FA were infused into the abomasum of lactating dairy cows in a 6 x 6 Latin square design with 21-d periods. Treatments were 1) control (no FA infused), 2) mostly saturated FA (C16:C18 = 0.72), 3) low-linoleic palm FA (C16:C18 = 0.85), 4) palm FA (C16:C18 = 0.72), 5) soy FA (C16:C18 = 0.10), and 6) high-palmitic soy FA (C16:C18 = 0.68). All treatments included meat solubles and Tween 80 as emulsifiers. Solid fat content (from 0 to 40 degrees C), melting point, and force at fracture were determined in butter oil. Milk fat from cows infused with palm FA (treatment 4) exhibited functionality equal to or better than control butter oil. Infusion with palm FA increased amounts of triglyceride (TG) fractions with 48, 52, and 54 carbon numbers but decreased TG with 32, 34, 36, and 42 carbon numbers. Infusion with soy FA increased TG with 26, 38, 40, 52, and 54 carbon numbers but decreased TG with 34, 42, and 46 carbons. Infusion of the mostly saturated FA increased TG with 38, 50, 52, and 54 carbon numbers but decreased TG with 32, 34, and 42 carbon numbers. These TG groups were consistently correlated with functional properties of butter oils from different treatments. The content of palmitic acid is important for maintaining functionality in the presence of increased polyunsaturated FA. The composition of milk fat may be able to be optimized through nutritional manipulation of diets for dairy cows if the optimal composition of FA and TG is defined for a particular dairy product.

  4. Subclinical hypocalcemia, plasma biochemical parameters, lipid metabolism, postpartum disease, and fertility in postparturient dairy cows.

    Science.gov (United States)

    Chamberlin, W G; Middleton, J R; Spain, J N; Johnson, G C; Ellersieck, M R; Pithua, P

    2013-01-01

    occurrence of clinical mastitis, ketosis, displaced abomasum, dystocia, retained placenta, metritis, or fertility measures (percentage cycling at 50-60 d postpartum, services per conception, or days open). These data suggest that early lactation fatty acid metabolism differs between cows with subclinical hypocalcemia and their normocalcemic counterparts.

  5. The efficiency of utilization of metabolizable energy and apparent absorption of amino acids in sheep given spring- and autumn-harvested dried grass.

    Science.gov (United States)

    Macrae, J C; Smith, J S; Dewey, P J; Brewer, A C; Brown, D S; Walker, A

    1985-07-01

    Three experiments were conducted with sheep given spring-harvested dried grass (SHG) and autumn-harvested dried grass (AHG). The first was a calorimetric trial to determine the metabolizable energy (ME) content of each grass and the efficiency with which sheep utilize their extra ME intakes above the maintenance level of intake. The second examined the relative amounts of extra non-ammonia-nitrogen (NAN) and individual amino acids absorbed from the small intestine per unit extra ME intake as the level of feeding was raised from energy equilibrium (M) to approximately 1.5 M. The third was a further calorimetric trial to investigate the effect of an abomasal infusion of 30 g casein/d on the efficiency of utilization of AHG. The ME content of the SHG (11.8 MJ/kg dry matter (DM] was higher than that of AHG (10.0 MJ/kg DM). The efficiency of utilization of ME for productive purposes (i.e. above the M level of intake; kf) was higher when given SHG (kf 0.54 between M and 2 M) than when given AHG (kf 0.43 between M and 2 M). As the level of intake of each grass was raised from M to 1.5 M there was a greater increment in the amounts of NAN (P less than 0.001) and the total amino acid (P less than 0.05) absorbed from the small intestines when sheep were given the SHG (NAN absorption, SHG 5.4 g/d, AHG 1.5 g/d, SED 0.54; total amino acid absorption SHG 31.5 g/d, AHG 14.3 g/d, SED 5.24). Infusion of 30 g casein/d per abomasum of sheep given AHG at M and 1.5 M levels of intake increased (P less than 0.05) the efficiency of utilization of the herbage from kf 0.45 to kf 0.57. Consideration is given to the possibility that the higher efficiency of utilization of ME in sheep given SHG may be related to the amounts of extra glucogenic amino acids absorbed from the small intestine which provide extra reducing equivalents (NADPH) and glycerol phosphate necessary for the conversion of acetate into fatty acids.

  6. Sawfly larval poisoning in cattle: Report on new outbreaks and brief review of the literature

    Directory of Open Access Journals (Sweden)

    Bianca Tessele

    2012-11-01

    Full Text Available Sawfly larval poisoning (SLP is an acute hepatotoxicosis documented in livestock in Australia, Denmark and in countries of South America. It is caused by the ingestion of the larval stage of insects of the suborder Symphyta, order Hymenoptera, commonly known as "sawfly". Three species of sawfly are reportedly involved in the toxicosis. The insect involved in Australian SLP is Lophyrotoma interrupta (Pergidae, in Denmark the cause of SLP is the ingestion of the larvae Arge pullata (Argidae, and in South American countries documented outbreaks of SLP were caused by the ingestion of yet another sawfly, Perreyia flavipes (Pergidae. In all geographical areas where it occurred, SLP causes important livestock losses. In cattle, as well as in other affected species, the disease has a short clinical course and in many outbreaks affected cattle can be found dead. When observed, clinical signs include apathy, recumbence, tremors, paddling movements and death in 24-48 hours. Neurological signs such aggressiveness attributable to hepatic encephalopathy are also observed. In cases with a more protracted course icterus and photodermatitis may develop. Gross findings included ascites, petechiae and ecchymosis over serosal surfaces of thoracic and abdominal cavities, and an enlarged liver that displays accentuation of the lobular pattern and edema of the gall bladder wall. Sawfly larval body fragments and heads are consistently found in the fore stomachs and occasionally abomasum of affected cattle. Main microscopic lesions are restricted to the liver and consist of centrolobular (periacinar to massive hepatocellular necrosis. In most lobules necrotic areas extended up to the portal triads where only a few viable hepatocytes remain. Mild to moderate lymphocyte necrosis is seen in lymphatic tissues. Cases occur in the winter months when the larval stages of the sawfly are developing. D-amino acid-containing peptides have been found to be the toxic principle in

  7. Inferring causal relationships between reproductive and metabolic health disorders and production traits in first-lactation US Holsteins using recursive models.

    Science.gov (United States)

    Dhakal, K; Tiezzi, F; Clay, J S; Maltecca, C

    2015-04-01

    Health disorders in dairy cows have a substantial effect on the profitability of a dairy enterprise because of loss in milk sales, culling of unhealthy cows, and replacement costs. Complex relationships exist between health disorders and production traits. Understanding the causal structures among these traits may help us disentangle these complex relationships. The principal objective of this study was to use producer-recorded data to explore phenotypic and genetic relationships among reproductive and metabolic health disorders and production traits in first-lactation US Holsteins. A total of 77,004 first-lactation daughters' records of 2,183 sires were analyzed using recursive models. Health data contained information on reproductive health disorders [retained placenta (RP); metritis (METR)] and metabolic health disorders [ketosis (KETO); displaced abomasum (DA)]. Production traits included mean milk yield (MY) from early lactation (mean MY from 6 to 60 d in milk and from 61 to 120 d in milk), peak milk yield (PMY), day in milk of peak milk yield (PeakD), and lactation persistency (LP). Three different sets of traits were analyzed in which recursive effects from each health disorder on culling, recursive effects of one health disorder on another health disorder and on MY, and recursive effects of each health disorder on production traits, including PeakD, PMY, and LP, were assumed. Different recursive Gaussian-threshold and threshold models were implemented in a Bayesian framework. Estimates of the structural coefficients obtained between health disorders and culling were positive; on the liability scale, the structural coefficients ranged from 0.929 to 1.590, confirming that the presence of a health disorder increased culling. Positive recursive effects of RP to METR (0.117) and of KETO to DA (0.122) were estimated, whereas recursive effects from health disorders to production traits were negligible in all cases. Heritability estimates of health disorders ranged

  8. Multiple-trait estimates of genetic parameters for metabolic disease traits, fertility disorders, and their predictors in Canadian Holsteins.

    Science.gov (United States)

    Jamrozik, J; Koeck, A; Kistemaker, G J; Miglior, F

    2016-03-01

    Producer-recorded health data for metabolic disease traits and fertility disorders on 35,575 Canadian Holstein cows were jointly analyzed with selected indicator traits. Metabolic diseases included clinical ketosis (KET) and displaced abomasum (DA); fertility disorders were metritis (MET) and retained placenta (RP); and disease indicators were fat-to-protein ratio, milk β-hydroxybutyrate, and body condition score (BCS) in the first lactation. Traits in first and later (up to fifth) lactations were treated as correlated in the multiple-trait (13 traits in total) animal linear model. Bayesian methods with Gibbs sampling were implemented for the analysis. Estimates of heritability for disease incidence were low, up to 0.06 for DA in first lactation. Among disease traits, the environmental herd-year variance constituted 4% of the total variance for KET and less for other traits. First- and later-lactation disease traits were genetically correlated (from 0.66 to 0.72) across all traits, indicating different genetic backgrounds for first and later lactations. Genetic correlations between KET and DA were relatively strong and positive (up to 0.79) in both first- and later-lactation cows. Genetic correlations between fertility disorders were slightly lower. Metritis was strongly genetically correlated with both metabolic disease traits in the first lactation only. All other genetic correlations between metabolic and fertility diseases were statistically nonsignificant. First-lactation KET and MET were strongly positively correlated with later-lactation performance for these traits due to the environmental herd-year effect. Indicator traits were moderately genetically correlated (from 0.30 to 0.63 in absolute values) with both metabolic disease traits in the first lactation. Smaller and mostly nonsignificant genetic correlations were among indicators and metabolic diseases in later lactations. The only significant genetic correlations between indicators and fertility

  9. Gastrointestinal parasites of sheep, municipality of Lajes, Rio Grande do Norte, Brazil Parasitos do trato gastrintestinal de ovinos, município de Lajes, Rio Grande do Norte, Brasil

    Directory of Open Access Journals (Sweden)

    Maria de Fátima de Souza

    2012-03-01

    Full Text Available This study investigated the gastrointestinal parasitism by helminths and protozoa in sheep (Ovis aries Santa Inês breed, municipality of Lajes, Rio Grande do Norte. Monthly, from April 2005 to August 2007, stool samples were collected from two tracer lambs in the first day of the experiment and performed a necropsy of these animals in 44th day. A total of 64 lambs were sampled, but only 62 lambs were slaughtered. The fecal samples were examined by sedimentation in water. The contents of the abomasum, small intestine and large intestine were examined for the recovery of helminths. The parasitological examination revealed eggs of the following groups of helminths: Strongyloidea, Strongyloides sp., Trichuris sp., and Moniezia sp. Also were found oocysts of Eimeria spp., cysts of Entamoeba ovis and Giardia duodenalis. The helminths identified from examining the contents were: Haemonchus contortus, Cooperia pectinata, Cooperia punctata, Trichostrongylus colubriformis, Moniezia expansa, Oesophagostomum sp. Skrjabinema ovis and Trichuris sp.O presente estudo investigou o parasitismo gastrintestinal por helmintos e protozoários em ovinos (Ovis aries da raça Santa Inês, no município de Lajes, Rio Grande do Norte. Mensalmente, entre abril de 2005 e agosto de 2007, foram coletadas amostras fecais de dois cordeiros traçadores no primeiro dia do experimento e realizada a necropsia desses animais no 44º dia. O total de cordeiros amostrados foi 64, mas apenas 62 foram necropsiados. As amostras fecais foram examinadas pela técnica de sedimentação espontânea em água. Os conteúdos do abomaso, intestino delgado e intestino grosso dos cordeiros necropsiados foram examinados para a recuperação dos helmintos. Os exames parasitológicos evidenciaram ovos dos seguintes grupos de helmintos: Strongyloidea, Strongyloides sp. , Trichuris sp., e Moniezia sp. Também foram encontrados oocistos de Eimeria spp., cistos de Entamoeba ovis e de Giardia duodenalis

  10. Growth performance, behaviour, forestomach development and meat quality of veal calves provided with barley grain or ground wheat straw for welfare purpose

    Directory of Open Access Journals (Sweden)

    Igino Andrighetto

    2010-01-01

    Full Text Available Two different feeding plans for veal calves were compared in the study: a traditional liquid diet supplemented with 250  g/calf/d of barley grain or with 250 g/calf/d of ground wheat straw. The two solid feeds had different chemical composi-  tion but a similar particle size obtained by grinding the straw in a mill with an 8-mm mesh screen. Twenty-four Polish  Friesian male calves were used in the study and they were housed in individual wooden stalls (0.83 x 1.80 m. The health  status of all the calves was satisfactory for the entire fattening period and no specific medical treatment was required  during the trial. Calves fed wheat straw showed a greater intake of solid feed (196 vs. 139 g/d; P  average daily gain (1288 vs. 1203 g/d; P  not affected by the type of solid feed and no milk refusal episodes were detected. The haemoglobin concentration was  similar in calves receiving the two feeding treatments despite the higher iron intake provided by the wheat straw through-  out the fattening period (2.12 vs. 1.15 g; P  calves’ metabolism. Feeding behaviour was affected by the provision of solid feeds. Eating and chewing were prolonged  in calves receiving ground wheat straw and the same solid feed reduced the frequency of oral stereotypies at the end of  the fattening period. At the slaughterhouse, no differences were observed between the feeding treatments as regards  carcass weight and dressing percentage. The calves fed ground wheat straw had a heavier weight of the empty omasum  (518 vs. 341 g; P  fed barley grain. The incidence of abomasal erosions, ulcers and scars was similar in both treatments; however the index  of abomasal damage, which considers the number and the seriousness of different type of lesions, was higher in calves  receiving barley grain. Therefore, the grinding of straw particles, as opposed to barley grain, can reduce the abrasive-  ness of roughage at the abomasum level. Visual evaluation of the

  11. Intoxicação por organofosforado em bovinos no Rio Grande do Sul Poisoning by organophosphate in cattle in southern Brazil

    Directory of Open Access Journals (Sweden)

    José C Oliveira-Filho

    2010-10-01

    Full Text Available Descreve-se um surto de intoxicação por organofosforado (ORF em bovinos na mesorregião centro ocidental do Rio Grande do Sul. A água fornecida a 49 bovinos foi contaminada com ORF e carbamato (CM. Vinte bovinos morreram após quadro clínico de poucas horas. Foram observados os clássicos sinais clínicos de hiperestimulação do sistema parassimpático incluindo incoordenação motora, agressividade, sialorreia, tremores musculares e, em alguns casos, diarreia. Na necropsia e histopatologia não foram observadas alterações morfológicas significativas. ORF e CM foram detectados por cromatografia em camada delgada em amostras de tecido de dois bovinos afetados. Adicionalmente, uma amostra da água consumida pelos bovinos foi positiva para ORF e CM e outras duas foram positivas para ORF. Uma análise quantitativa realizada por cromatografia gasosa no conteúdo do abomaso de um bovino afetado, revelou 0,93µg/g de mancozebe (CM e 0,07 µg/g de forato (ORF.An outbreak of organophosphate (ORF poisoning in cattle occurred in the central-midwestern region of Rio Grande do Sul, Brazil. The water available for 49 cattle was contaminated with ORF and carbamate (CM. Twenty cows died after a clinical course that lasted for few hours. The classical clinical signs of over stimulation of the parasympathetic nervous system were observed, including motor incoordination, aggressiveness, drooling, muscle tremors and, on occasion, diarrhea. Significant morphological changes were not observed at necropsy or at histopathological examination. ORF e CM were detected by thin layer chromatography in tissue samples of two affected cattle and in a sample from the water consumed by the affected cattle. Additionally, two other water samples accessed by the affected cattle were positive for ORF. A quantitative analysis carried out by gas chromatography in the abomasum contents of an affected cow revealed 0.93µg/g of mancozebe (CM and 0.07µg/g of phorate (ORF.

  12. Study of the effect of presence or absence of protozoa on rumen fermentation and microbial protein contribution to the chyme.

    Science.gov (United States)

    Belanche, A; Abecia, L; Holtrop, G; Guada, J A; Castrillo, C; de la Fuente, G; Balcells, J

    2011-12-01

    bacterial-N flow through the abomasum by 33%, whereas the protozoa-N contribution to the microbial N flow increased from 1.9 to 14.1% when barley grain was added to the alfalfa hay. Absolute data related to intestinal flow must be treated with caution because the limitations of the sampling and maker system employed.

  13. Effects of folic acid and vitamin B12 supplementation on culling rate, diseases, and reproduction in commercial dairy herds.

    Science.gov (United States)

    Duplessis, M; Girard, C L; Santschi, D E; Laforest, J-P; Durocher, J; Pellerin, D

    2014-01-01

    This study was undertaken to determine the effect of a combined folic acid and vitamin B12 supplement given in early lactation on culling rate, metabolic disorders and other diseases, and reproduction in commercial dairy herds. A total of 805 cows (271 primiparous and 534 multiparous cows) in 15 commercial dairy herds were involved. Every 2mo from February to December 2010 and within each herd, cows were assigned according to parity, previous 305-d milk production, and calving interval to 5mL of either (1) saline 0.9% NaCl (control group) or (2) 320mg of folic acid + 10mg of vitamin B12 (vitamin group). Treatments were administered weekly by intramuscular injections starting 3wk before the expected calving date until 8wk after parturition. A total of 221 cows were culled before the next dry period. Culling rate was not affected by treatment and was 27.5%; culling rate was greater for multiparous (32.2%) than for primiparous cows (18.8%). Within the first 60d in milk (DIM), 47 cows were culled, representing 21.3% of total culling, and no treatment effect was noted. Ketosis incidence based on a threshold ≥100µmol/L of β-hydroxybutyrate in milk was 38.3±2.9% for the vitamin group and 41.8±3.0% for the control group and was not affected by treatment. The combined supplement of folic acid and vitamin B12 did not decrease incidence of retained placenta, displaced abomasum, milk fever, metritis, or mastitis. However, the incidence of dystocia decreased by 50% in multiparous cows receiving the vitamin supplement, although no effect was observed in primiparous cows. The first breeding postpartum for multiparous cows occurred 3.8d earlier with the vitamin supplement compared with controls, whereas no treatment effect was seen for primiparous cows. Days open, first- and second-breeding conception rates, number of breedings per conception, and percentage of cows pregnant at 150 DIM were not affected by treatment. The reduced percentage of dystocia combined with the

  14. Dry period plane of energy: Effects on feed intake, energy balance, milk production, and composition in transition dairy cows.

    Science.gov (United States)

    Mann, S; Yepes, F A Leal; Overton, T R; Wakshlag, J J; Lock, A L; Ryan, C M; Nydam, D V

    2015-05-01

    The objective was to investigate the effect of different dry cow feeding strategies on the degree of ketonemia postpartum. Epidemiologic studies provide evidence of an association between elevated β-hydroxybutyrate (BHBA) concentrations in postpartum dairy cows and a decreased risk for reproductive success as well as increased risk for several diseases in early lactation, such as displacement of the abomasum and metritis. The plane of energy fed to cows in the prepartum period has been shown to influence ketogenesis and the degree of negative energy balance postpartum. Our hypothesis was that a high-fiber, controlled-energy diet (C) fed during the dry period would lead to a lower degree of hyperketonemia in the first weeks postpartum compared with either a high-energy diet (H), or a diet where an intermediate level of energy would only be fed in the close-up period (starting at 28d before expected parturition), following the same controlled-energy diet in the far-off period. Hyperketonemia in this study was defined as a blood BHBA concentration of ≥1.2mmol/L. Holstein cows (n=84) entering parity 2 or greater were enrolled using a randomized block design and housed in individual tiestalls. All treatment diets were fed for ad libitum intake and contained monensin. Cows received the same fresh cow ration after calving. Blood samples were obtained 3 times weekly before and after calving and analyzed for BHBA and nonesterified fatty acids (NEFA). Milk components, production, and dry matter intake were recorded and energy balance was calculated. Repeated measures ANOVA was conducted for the outcomes dry matter intake, energy balance, BHBA and NEFA concentrations, milk and energy-corrected milk yield, as well as milk composition. Predicted energy balance tended to be less negative postpartum in group C and cows in this group had fewer episodes of hyperketonemia compared with both the intermediate group and group H in the first 3 wk after calving. Postpartum BHBA and

  15. In vivo assessment of the ability of condensed tannins to interfere with the digestibility of plant protein in sheep

    Energy Technology Data Exchange (ETDEWEB)

    Andrabi, S.M.; Ritchie, M.M.; Stimson, C.; Horadagoda, A.; Hyde, M.; McNeill, D.M. [MC Franklin Laboratory, Faculty of Veterinary Science, University of Sydney, Camden, NSW (Australia)]. E-mail: karalue@bigpond.net.au

    2005-08-19

    To assess the effect of condensed tannin (CT) astringency on the digestibility of protein post-ruminally, CT were purified from four types (accessions) of Mulga (Acacia aneura), and a Leucaena pallida and dosed into sheep as complexes with {sup 15}N-plant protein (tannin-protein complexes, TPC), together with an indigestible marker, chromium-EDTA (Cr-EDTA). Each CT treatment dose comprised 12 mg CT, 10 mg {sup 15}N-protein, and 2.77 mg of Cr. A protein-only control (same as the other TPC solutions but without any CT) treatment was also included to make a total of six treatments. Treatments were applied in two 6 x 6 Latin Square designs with 72 h between each infusion for each sheep. In the first, the solutions were infused post-ruminally via an abomasal cannula. In the second they were dosed directly into the mouth following an intra-nasal dose of an analogue of vasopressin in an attempt to stimulate the oesophageal groove reflex to direct the solutions more efficiently toward the small intestine. Results showed no detectable effect of CT type on the in vivo digestibility of the {sup 15}N-protein. Protein digestibilities were uniformly high, indicative of complete dissociation of the TPC. There was no correlation between protein digestibility, mouth to faeces, and protein digestibility, abomasum to faeces (P > 0.05). In vivo digestibility was also uncorrelated with CT astringencies defined in vitro (P > 0.05). Astringency in vitro was defined as the mg of CT required to achieve half-maximal precipitation of 0.5 mg of protein (bovine serum albumin). In vitro, the most astringent CT (A. aneura 883558), had at least 1.6 times the astringency of the weakest CT (A. aneura 842394). The A. aneura with the weakest CT also contained less than 1/3 the total amount of CT/g leaf dry matter than that with the strongest, highlighting the scope for selection of more nutritionally useful types of A. aneura. Limitations of the in vivo protocol used are discussed and it is concluded

  16. Fetal organ growth in response to oesophageal infusion of amniotic fluid, colostrum, milk or gastrin-releasing peptide: a study in fetal sheep.

    Science.gov (United States)

    Trahair, J F; Sangild, P T

    2000-01-01

    The hypothesis of the present study was that the infusion of the biological fluids to which the developing gut is normally exposed (i.e. amniotic fluid, colostrum, milk) and a single growth factor (gastrin-releasing peptide), which is found in high concentrations in fetal fluids and milk, could ameliorate the altered growth induced by the elimination of swallowed input secondary to ligation of the oesophagus. At 108-110 days of gestation the fetal oesophagus was ligated and a catheter inserted towards the stomach (32 fetuses). At 117-119 days of gestation saline (n = 5), amniotic fluid (n = 5), colostral whey (n = 5), milk whey (n = 5) or gastrin-releasing peptide (3.6 nmol day(-1), n = 6), was infused for 7 days (4 x 20 mL day(-1)), or no infusion was given (ligated group, n = 6). A further 15 fetuses were not ligated (normal group, n = 15). All fetuses had carotid artery and/or jugular vein catheters implanted. At 124-126 days of gestation the fetus was delivered and fetal body and organ weights recorded. Analysing the results by ANOVA, there were no effects of either ligation alone or infusion after ligation on fetal weight, crown-rump length, or weight relative to bodyweight of heart, adrenal, pancreas, large intestine and cecum. There were significant differences between the infusion groups for lungs, kidney, pancreas, total gut, abomasum, small intestine, spleen, chest and neck thymus, and mesenteric lymph nodes. Ligation alone significantly reduced small intestinal growth and increased kidney and spleen growth. Colostrum infusion enhanced growth of most organs. Gastrin-releasing peptide significantly increased growth of all the immune organs studied. It was concluded that at an age when premature delivery could be encountered, the fetal gut is capable of significant adaptive growth, to varying degrees, depending on the enteral diet. Growth effects in organs distant to the gut suggest that either gastrointestinal uptake and transport of growth factors or

  17. 脑红蛋白在成年牦牛不同组织细胞中的分布%Distribution of neuroglobin in different tissues and cells of the adult yak

    Institute of Scientific and Technical Information of China (English)

    曹亮; 刘霞; 石宁宁; 李盛杰; 杜晓华

    2013-01-01

    Objective To explore the localization of neuroglobin (NGB) in adult yak tissues and cells. Methods Eight health adult yaks were used in this study. NGB expression in adult yak tissues and cells was localized using the immunohistochemical SP technique. Results NGB-positive cells were mainly distributed in nerve cells, adrenal gland, adenohypophysis and pancreas islet, testis, efferent ductules of testis and epididymal epithelium of genital system. NGB-immunoreactive product was located in the cytoplasm. The NGB-positive cells were found in the fundic glands of abomasum and duodenal glands. Conclusion The high expression of NGB in tissues and cells of nervous system, endocrine system, genital system and alimentary system in yak suggested that NGB might play an important role in the utilization of oxygen and physiological functions.%目的 探讨脑红蛋白(NGB)在成年牦牛不同组织细胞中的分布特征.方法 选择健康成年牦牛8只,利用免疫组织化学染色SP法,观察NGB在成年牦牛不同组织细胞中的分布特征.结果 在成年牦牛体内,NGB主要表达于神经细胞,肾上腺、腺垂体及胰岛组织细胞,雄性生殖系统睾丸、睾丸输出小管及附睾管上皮细胞.NGB免疫阳性物质定位于细胞质.本研究在哺乳动物消化系统皱胃胃底腺分泌细胞及十二指肠腺分泌细胞中发现有NGB阳性表达.结论 NGB在牦牛神经系统、内分泌系统、生殖系统以及消化系统组织细胞中有高表达,提示NGB在这些组织的氧利用及功能活动中发挥作用.

  18. Valine partitioning and kinetics between the gastrointestinal tract and hind limbs in lambs with an adult Trichostrongylus colubriformis burden.

    Science.gov (United States)

    Bermingham, E N; McNabb, W C; Sinclair, B R; Tavendale, M H; Roy, N C

    2011-11-01

    Intestinal parasitic infection increases the demand for AA because of increased protein synthesis in the intestine and increased luminal losses of AA, and these increased demands may be supported by increased mobilization of AA from the skeletal muscles. Two experiments were conducted to determine the effects of parasitic infection on valine kinetics within the gastrointestinal tract and hind limbs of lambs fed fresh forages. On d 1, lambs were given 6,000 stage-3 Trichostrongylus colubriformis larvae per day for 6 d (n = 6) or kept as parasite-free controls (n = 6) and fed fresh lucerne (Medicago sativa; Exp. 1) or fresh sulla (Hedysarum coronarium; Exp. 2). On d 48, valine kinetics within the mesenteric- (MDV) and portal-drained viscera (PDV) and hind limbs were obtained by carrying out concurrent infusions of para-amminohippuric acid into the mesenteric vein and indocyanin green into the abdominal aorta (for blood flow), and [3,4-(3)H]valine into the jugular vein and [1-(13)C]valine into the abomasum for 8 h (for kinetics). During the infusions, blood was collected from the mesenteric and portal veins and from the mesenteric artery and vena cava, and plasma was harvested. After the 8-h infusion, lambs were euthanized, ileal digesta were collected, and tissues were sampled from the intestine and muscle (biceps femoris). Tissues, digesta, and plasma were analyzed for valine concentration, specific radioactivity, and isotopic enrichment. In both experiments, intestinal worm burdens on d 48 were greater in parasitized lambs (P = 0.0001 and 0.003). In Exp. 1, parasitic infection increased (P = 0.03) the total valine irreversible loss rate (ILR) in the MDV and PDV. In Exp. 2, luminal ILR of valine in the MDV was reduced (P = 0.01); however, ILR of valine in the PDV was unaffected. Despite these changes within the MDV and PDV, parasitic infection did not affect the ILR of valine within the hind limbs, and valine transport rates were largely unchanged. We suggest that

  19. Genetic selection for health traits using producer-recorded data. I. Incidence rates, heritability estimates, and sire breeding values.

    Science.gov (United States)

    Zwald, N R; Weigel, K A; Chang, Y M; Welper, R D; Clay, J S

    2004-12-01

    The objective of this study was to determine the feasibility of genetic selection for health traits in dairy cattle using data recorded in on-farm herd management software programs. Data regarding displaced abomasum (DA), ketosis (KET), mastitis (MAST), lameness (LAME), cystic ovaries (CYST), and metritis (MET) were collected between January 1, 2001 and December 31, 2003 in herds using Dairy Comp 305, DHI-Plus, or PCDART herd management software programs. All herds in this study were either participants in the Alta Genetics (Watertown, WI) Advantage progeny testing program or customers of the Dairy Records Management Systems (Raleigh, NC) processing center. Minimum lactation incidence rates were applied to ensure adequate reporting of these disorders within individual herds. After editing, DA, KET, MAST, LAME, CYST, and MET data from 75,252 (313), 52,898 (250), 105,029 (429), 50,611 (212), 65,080 (340), and 97,318 (418) cows (herds) remained for analysis. Average lactation incidence rates were 0.03, 0.10, 0.20, 0.10, 0.08, and 0.21 for DA, KET, MAST, LAME, CYST, and MET (including retained placenta), respectively. Data for each disorder were analyzed separately using a threshold sire model that included a fixed parity effect and random sire and herd-year-season of calving effects; both first lactation and all lactation analyses were carried out. Heritability estimates from first lactation (all lactation) analyses were 0.18 (0.15) for DA, 0.11 (0.06) for KET, 0.10 (0.09) for MAST, 0.07 (0.06) for LAME, 0.08 (0.05) for CYST, and 0.08 (0.07) for MET. Corresponding heritability estimates for the pooled incidence rate of all diseases between calving and 50 d postpartum were 0.12 and 0.10 for the first and all lactation analyses, respectively. Mean differences in PTA for probability of disease between the 10 best and 10 worst sires were 0.034 for DA, 0.069 for KET, 0.130 for MAST, 0.054 for LAME, 0.039 for CYST, and 0.120 for MET. Based on the results of this study, it

  20. Administração de somatotropina bovina no período pré-parto sobre parâmetros produtivos, sanitários e reprodutivos da primeira lactação de vacas holandesas Bovine somatotropin administration during pre-delivery period to productive, sanitary and reproductive parameters of holstein cows' first lactation

    Directory of Open Access Journals (Sweden)

    Laerte Dagher Cassoli

    2002-04-01

    Full Text Available Para avaliar a administração de somatotropina bovina (bST no período pré-parto, sobre o desempenho na primeira lactação, foram utilizadas 18 novilhas gestantes, de modo que 9 receberam injeções subcutâneas de 500mg de bST nos dias 21 e 9 antes da data prevista para o parto. Os animais foram alimentados com dieta total, 2 vezes ao dia, e os dados coletados foram relativos a parâmetros sanitários, produtivos e reprodutivos na primeira lactação. A administração de bST não afetou os parâmetros sanitários (retenção de placenta, metrite, deslocamento de abomaso, febre do leite e cetose e reprodutivo (cisto folicular. Entretanto, o uso de bST no pré-parto levou à variação de escore de condição corporal (ECC 65% maior, adiantou o pico de produção de leite em 9,7 dias e diminuiu os dias em lactação em 21,8 dias em relação ao controle (P The effects of pre-delivery administration of bovine somatotropin (bST on first lactation performance were evaluated in this study, which involved eighteen Holstein pregnant heifers. Nine animals were supplemented with subcutaneous injections of 500 mg of bST, on days 21 and 9 prior expected delivery, and 9 were not supplemented (control group. Animals were fed total mixed ration, twice daily. Data evaluated in this experiment were related to sanitary, reproductive and productive parameters during first lactation. Administration of bST did not affect parameters related to sanity (retained placenta, metritis, displacement abomasum, milk fever, ketosis, reproduction (follicular cysts. However, heifers supplemented with bST showed changes of body condition score 65% greater, anticipated milk production peak in 9.7 days and decreased number of days in lactation in 21,8 days, compared to the non-supplemented ones (p < 0.05. Milk production peak and milk production corrected for 305 days were not affected by bST administration.

  1. Effects of Graded Abomasal Starch Infusion on Feed Intake, Lactation Performance,Gastrointestinal Fermentation and Blood Metabolism of Lactating Cows%真胃灌注不同水平淀粉对泌乳后期奶牛采食量、泌乳性能、胃肠道发酵和血液代谢的影响

    Institute of Scientific and Technical Information of China (English)

    邹杨; 李胜利; 曹志军; 杨占山

    2014-01-01

    This experiment was performed to assess the effects of infusion of graded starch in abomasum on feed intake,lactation performance,gastrointestinal fermentation and blood metabolism of lactating cows. Four Holstein cows fitted with permanent ruminal cannulas were arranged into four groups by a 4×4 Latin square de-sign. Cows were infused with 0,800,1 600 and 2 400 g / d starch in abomasum,respectively. The experiment lasted for 7 days,which was consisted of a 5-day pre-experimental period and a 2-day experimental period. The results showed as follows:1)dry matter intake of dairy cows was not affected by abomasal starch infusion at different levels(P>0.05);with the increase of abomasal starch infusion level,digestive energy intake( P =0.022)and net energy for lactation intake(P = 0.014)were linearly increased,while starch apparent digesti-bility was linearly decreased(P = 0.029). 2)With the increase of abomasal starch infusion level,milk fat per-centage(P = 0.024)and yield(P = 0.022)were quadratically increased,those in 1 600 g / d group reached the highest. 3)Butyric acid concentration in fecal fluid in 800 g / d group was significantly lower than that in 1 600 and 2 400 g / d groups(P0.05). In conclusion,milk fat percentage is quadratically increased with the increase of abomasal starch infusion level,and the peak amount of rumen es-caped starch that can be utilized by dairy cows at late lactation period is 1 600 g / d.%为了研究真胃灌注不同水平淀粉对泌乳后期奶牛采食量、泌乳性能、胃肠道发酵和血液代谢的影响,采用4×4拉丁方设计将4头安装有永久性瘤胃瘘管的荷斯坦2胎奶牛分为4组,分别向真胃内灌注0(对照组)、800、1600、2400 g / d 淀粉。共进行4期试验,每期7 d(5 d的预试期和2 d 的正试期)。结果表明:1)真胃灌注不同水平淀粉对奶牛干物质采食量无显著影响(P>0.05);奶牛的消化能( P =0.022)和泌乳净能采食量( P

  2. Eficiência microbiana, fluxo de compostos nitrogenados no abomaso, amônia e pH ruminais, em bovinos recebendo dietas contendo feno de capim-tifton 85 de diferentes idades de rebrota Microbial efficiency, abomasal nitrogen compounds flow, ruminal ammonia and ruminal pH in cattle fed diets containing tifton 85 bermudagrass hays at different regrowth ages

    Directory of Open Access Journals (Sweden)

    Karina Guimarães Ribeiro

    2001-04-01

    Full Text Available Avaliaram-se a eficiência de síntese microbiana, o fluxo de compostos nitrogenados no abomaso, o balanço de compostos nitrogenados, a taxa de passagem da digesta ruminal, a concentração de amônia e o pH ruminais, em bovinos recebendo rações contendo feno de capim-tifton 85 de diferentes idades de rebrota. Utilizaram-se quatro animais zebu, com peso médio de 340 kg, fistulados no rúmen e abomaso, distribuídos em um delineamento em quadrado latino 4 x 4. Todas as rações continham 60% de volumoso e 40% de concentrado. O volumoso foi constituído de feno de capim-tifton 85 de 28, 35, 42 e 56 dias de idade e o concentrado continha fubá de milho e mistura mineral. Os microorganismos ruminais foram quantificados utilizando-se as bases purinas como indicador. O pH e N-amoniacal foram mensurados, no fluido ruminal, antes e 2; 4 e 6 horas após o fornecimento da ração. A taxa de passagem foi determinada pelo modelo unicompartimental, utilizando-se o óxido crômico como indicador. As eficiências de síntese microbiana não foram influenciadas pela idade do feno na ração, apresentando valores médios de 31,32 g Nbact/kg MODR; 30,74 g Nbact/kg CHODR; 337,4 g MSbact/kg CHODR; e 12,5 g PBbact/100 g NDT. Estimaram-se máximos fluxos de compostos nitrogenados totais, amoniacais e não-amoniacais, de 119,0; 9,76; e 109,6 g/dia, com a inclusão de feno com 39,7; 37,6; e 39,9 dias de idade, respectivamente, e fluxo de compostos nitrogenados bacterianos de 80,54 g/dia, em média. O balanço de nitrogênio, a taxa de passagem, as concentrações de amônia e o pH ruminais também não foram influenciados pela idade do feno na ração, encontrando-se valores de 30,67 g/dia; 3,2%/h; 9,7 mg/100mL (máximo às 1,38h e 6,08 (mínimo às 6,64h, respectivamente.The microbial efficiency synthesis, the abomasum nitrogen compounds flow, the nitrogen compounds balance, the passage rate of ruminal digest, the ruminal ammonia concentration and ruminal pH in

  3. Intoxicação por Baccharidastrum triplinervium (Asteraceae em bovinos Poisoning in cattle by Baccharidastrum triplinervium (Asteraceae

    Directory of Open Access Journals (Sweden)

    Ingeborg Maria Langohr

    2005-12-01

    Baccharidastrum triplinervium in cattle from Paraná, Brazil, is described. The disease occurred during a severe drought in early summer. The onset of clinical signs was two days after 50 cows and 8 heifers had been introduced into a pasture with high density of B. triplinervium that showed signs of having been consumed by the animals. Fifteen animals (9 cows and 6 heifers got sick. Of these 15, two cows and four heifers died after a clinical course of 12-60 hours. Clinical signs included depression, ruminal atony, moderate bloat, marked dehydration, mild diarrhea and anorexia. The animals were restless, laying down and getting up constantly, remaining progressively longer periods in sternal recumbency. Once in that position, the animals had an extended head or the head turned to one of the sides of the body, and were groaning. Additionally, the cows had an abrupt fall in milk yield. The remaining affected cattle presented milder clinical signs and were partially back to their feed on the day following the onset of the clinical signs. The milk production was back to normal values within one week. The main gross lesions observed in two necropsied cows were in the forestomachs and abomasum, consisting of edema of the ruminal wall, as well as of diffuse reddening of the mucosae of the rumen, reticulum, abomasum and of some of the omasal folds. The main histological lesions included multifocal ballooning degeneration and necrosis of the lining epithelium of the rumen, associated with neutrophilic infiltrate. The diagnosis was based on the epidemiological data and on the experimental reproduction of the disease by force-feeding 3 bovine with the aeral fresh parts (20 and 30g/kg of B. triplinervium. Chemical analysis of dried material from B. triplinervium harvested at the site of the outbreak was negative for macrocyclic trichothecenes.

  4. Nutrition, microbiota, and endotoxin-related diseases in dairy cows Nutrição, microbiota e doenças relacionadas à endotoxina em vacas leiteiras

    Directory of Open Access Journals (Sweden)

    Burim N. Ametaj

    2010-07-01

    Full Text Available In this review article we present an overall summary of the role that high-grain/low forage diets have on rumen composition of microbiota and how changes in the diet affect the release of bacterial cell wall components that are toxic to the host. One of these toxic compounds is lipopolysaccharide or endotoxin, a component of the outer membrane of all Gram-negative bacteria. Moreover, data are provided that support the concept that endotoxin translocates into the blood circulation and show that rumen endotoxin is associated with multiple perturbations of blood variables related to carbohydrate, lipid, and mineral metabolism. In addition, endotoxin induces a general, nonspecific immune response known as acute phase response. We also pinpoint the fact that high-grain diets are associated with distinct clusters of plasma metabolites and immune variables suggesting that changing cereal grain to forage ratio in the diet is very important for the health of dairy cattle. Furthermore, we provide information that support the concept that endotoxin is involved in multiple metabolic diseases such as fatty liver, milk fever, laminitis, retained placenta, displaced abomasum, and downer cow syndrome. More research is warranted to clarify the mechanisms by which nutrition, microbiota, and endotoxin contribute to development of metabolic diseases in dairy cattle. It is concluded that besides the aforementioned causal agents other compounds generated in the gastrointestinal tract such as lipoteichoic acid or methylated amines might be involved in the etiology of several metabolic diseases.Neste artigo de revisão é apresentado um resumo total do papel que a dieta de alto teor de grãos e baixo teor de forragem tem sobre a composição da microbiota do rúmen e como as mudanças na dieta afetam a liberação de componentes da parede celular bacteriana tóxicos ao hospedeiro. Um destes compostos tóxicos é um lipopolisacarídeo ou endotoxina, um componente da

  5. Effects of Heutral Detergent Fiber Level of Starter on Growth Performance and Digestive Tract Development of Suckling Lambs%开食料中性洗涤纤维水平对哺乳羔羊生长性能和消化道发育的影响

    Institute of Scientific and Technical Information of China (English)

    王世琴; 李冲; 李发弟; 汪晓娟; 王维民; 刘婷

    2014-01-01

    In order to investigate the effects of neutral detergent fiber( NDF)level of starter on growth per-formance and digestive tract development of suckling lambs,twenty-four healthy new born male Hu lambs with similar birth weight[(3.01±0.12)kg]were divided into 4 groups with 6 lambs in each group by a one factor design. Lambs in control group were ewe reared without dietary supplementation,and those in high,medium and low NDF groups were ad libitum fed starter with 17.01%,13.65% and 11.32% NDF,respectively,based on ewe reared from 7 days of age. All lambs were slaughtered at 35 days of age to determine weight and the volume of each part of digestive tract and the length of each part of intestinal tract. The results showed as fol-lows:1)final weight,average daily gain and carcass weight of high NDF group were significantly higher than those of control group( P<0.05),and carcass weight of high NDF group was significantly higher than that of low NDF group(P<0.05). 2)Abomasum weight of high,medium and low NDF groups was significantly higher than that of control group( P<0.05),and reticulorumen volume of high NDF group was significantly higher than that of control group(P<0.05);abomasum weight of high NDF group was significantly higher than that of control group and low NDF group( P<0.05). 3)Jejunum weight of high and low NDF groups was significantly higher than that of control group( P<0.05),and jejunum volume of high NDF group was signifi-cantly higher than that of control group(P<0.05). It is concluded that the optimal NDF level of starter for suckling lambs is 17.01% under the conditions in the present study,and lambs can obtain best growth perform-ance and digestive tract development under the above level.%为研究开食料中性洗涤纤维( NDF)水平对1~35日龄羔羊生长性能和消化道发育的影响,本试验选用24只体况良好、初生重[(3.01±0.12)kg]相近的湖羊初生公羔,采用单因素随机分组设计分为4

  6. Intoxicação por Cestrum laevigatum (Solanaceae em bubalinos Poisoning by Cestrum laevigatum (Solanaceae in buffaloes

    Directory of Open Access Journals (Sweden)

    José Diomedes Barbosa

    2010-12-01

    ões necrosadas observou-se um halo de hepatócitos com vacuolização.Based on the history and clinical and pathological data, as well as on inspection of the pastures, a mortality of buffaloes in the county of Itaguaí/RJ, Brazil, was diagnosed as poisoning by Cestrum laevigatum Schlecht., a plant of the Solanaceae family. The poisoning was reproduced in two buffaloes. Dried leaves of the shrub were administered by hand, in single doses corresponding to 20g/kg and 40g/kg of the fresh leaves, to four buffaloes of the Murrah breed. The dose corresponding to 40g/kg of the fresh leaves caused signs of poisoning, as apathy, anorexia, absence of rumen movements, dysmetria, excitement and aggressiveness, followed by death of the two buffaloes within 65 hours after administration. From the two buffaloes that received the corresponding dose of 20g/kg of the fresh plant, one presented clinical signs characterized mainly by decrease of the rumen movements, but recovered 97h22min after the administration; the other buffalo did not show symptoms of poisoning. Laboratory analyses for biochemical evaluation accused hepatic alterations. In one buffalo that died, the main macroscopic finding was an orange liver with a clear nutmeg appearance; in the second buffalo, the orange liver had no nutmeg appearance. Other alterations found in these two buffaloes were slight edema of the gall bladder wall, a slightly reddish mucous membrane of the abomasum, extensive echymoses in the endocard of the left ventricle and few petechiae in the endocard of the right ventricle; the abomasum content was slightly dry, and the large intestine had little and slightly dry contents wrapped by mucus. Histopatological examination revealed severe coagulative necrosis of the liver parenchyma in the centrolobular and intermediate lobular areas, with a halo of vacuolated hepatocytes at the periphery of the necrotic areas.

  7. Effect of lipid supplementation on milk odd- and branched-chain fatty acids in dairy cows.

    Science.gov (United States)

    Baumann, E; Chouinard, P Y; Lebeuf, Y; Rico, D E; Gervais, R

    2016-08-01

    Eight ruminally fistulated, multiparous Holstein cows were arranged in a double 4×4 Latin square with 14-d periods to investigate the effects of lipid supplementation on performance, rumen parameters, the milk odd- and branched-chain fatty acid (OBCFA) profile, and the relationships between milk OBCFA and rumen parameters. Lipid supplementation is known to inhibit microbial growth in the rumen, decrease de novo microbial fatty acid synthesis, and increase the uptake of circulating fatty acids by the mammary gland; treatments were selected to isolate these effects on the milk OBCFA profile. The 4 treatments were (1) a lipid-free emulsion medium infused in the rumen (CTL), (2) soybean oil as a source of polyunsaturated fatty acids infused in the rumen (RSO), (3) saturated fatty acids (38% 16:0, 40% 18:0) infused in the rumen (RSF), and (4) saturated fatty acids infused in the abomasum (ASF). Fat supplements were provided continuously as emulsions at a rate of 450g/d. Preplanned contrasts compared CTL to RSO, RSO to RSF, and RSF to ASF. Infusing RSO slightly decreased ruminal pH, but did not affect volatile fatty acids profile and milk fat concentration as compared with CTL. The yields of energy-corrected milk, fat, and protein were greater with RSF compared with RSO. The concentration of odd-chain fatty acids was decreased by RSO, whereas even-chain iso fatty acids were not affected. Milk fat concentration of 17:0 + cis-9 17:1 was higher for RSF than for RSO, due to the saturated fatty acids supplement containing 2% 17:0 + cis-9 17:1. Limited differences were observed in the milk OBCFA profile between RSF and ASF. A multiple regression analysis yielded the following equation for predicting rumen pH based on milk fatty acids: pH=6.24 - (0.56×4:0) + (1.67 × iso 14:0) + (4.22 × iso 15:0) + (9.41×22:0). Rumen propionate concentration was negatively correlated with milk fat concentration of iso 14:0 and positively correlated with milk 15:0, whereas the acetate

  8. Simulação e validação de parâmetros da cinética digestiva em novilhos mestiços suplementados a pasto, por intermédio do sistema in vitro de produção de gases Simulation and validation of digestive kinetic parameters using an in vitro gas production system in crossbred steers with pasture supplementation

    Directory of Open Access Journals (Sweden)

    Edenio Detmann

    2005-12-01

    production technique. In vivo estimates were obtained from five Holstein x Zebu steers averaging 304 kg of body weight and 24 months of age that were grazing on five paddocks of Brachiaria decumbens (0.34 ha. The supplements fed to the steers (4 kg/animal/day contained: grounded corn, whole soybean, urea, ammonium sulfate, and mineral salt and the following crude protein levels: 12, 16, 20, or 24% on as-fed basis. Statistical analyses were performed as a 4 x 4 Latin square design with 21-days periods. The fifth animal was not supplemented and was used only for descriptive comparisons. Data were obtained by regression of values predicted in vitro on values observed in vivo. Use of digestion rates obtained in vitro resulted in under- and over-estimation of the ruminal degradation of fiber (FC and nonfiber (NFC carbohydrates with overall biases of -25.27% and +33.58%, respectively. No significant relationship was found between predicted versus observed values for microbial efficiency showing lack of relationship between predicted and observed values for the abomasum flow of microbial nitrogen. Results showed that the ruminal incubation of feeds separately underestimates the digestion rate of FC.

  9. Polymerase chain reaction for the diagnosis of bovine genital campylobacteriosis Reação em cadeia da polimerase para o diagnóstico de campilobacteriose genital bovina

    Directory of Open Access Journals (Sweden)

    Ana C.M. Groff

    2010-12-01

    Full Text Available Bovine genital campylobacteriosis is a common venereal disease of cattle; the prevalence of this disease can be underestimated mostly because of the nature of the etiological agent, the microaerobic Campylobacter fetus subspecies venerealis. The purpose of the current study was to evaluate the utilization of polymerase chain reaction (PCR in the diagnosis of genital campylobacteriosis in samples obtained from bull prepuce aspirate, cow cervical mucus, and abomasum contents of aborted fetuses, collected into enrichment medium. Five different DNA extraction protocols were tested: thermal extraction, lysis with proteinase K, lysis with guanidine isothiocyanate, lysis with DNAzol, and lysis with hexadecyltrimethylammonium bromide (CTAB. The specificity, sensitivity, and technical application of the PCR assay were also evaluated with clinical samples and compared to bacterial isolation by standard culture. DNA extraction by the CTAB protocol provided better results in PCR, and it was able to detect 63 colony-forming units per ml of C. fetus. Out of 277 clinical samples tested, 68 (24% were positive for Campylobacter fetus using PCR, while only 8 (2.8% of the samples were positive by bacterial isolation in solid medium, proving the superiority of the PCR technique when compared to the standard isolation method, and providing evidence for its usefulness as a better screening test in cattle for the diagnosis of bovine genital campylobacteriosis.Campilobacteriose genital bovina é uma doença venérea comum em bovinos. A prevalência desta doença pode ser subestimada na maioria das vezes pela natureza microaeróbica do agente etiológico, Campylobacter fetus subspecies venerealis. O propósito do presente estudo foi avaliar a utilização da reação de polimerase em cadeia (PCR no diagnóstico de campilobacteriose genital em amostras obtidas de aspirado prepucial de touros, muco cervical de vacas e conteúdo abomasal de fetos abortados, coletados em

  10. Total and partial digestibility, rates of digestion obtained with rumen evacuation and microbial protein synthesis in bovines fed fresh or ensiled sugar cane and corn silage Digestibilidade total e parcial, taxas de digestão obtidas com o esvaziamento ruminal e síntese de proteína microbiana em bovinos alimentados com cana-de-açúcar fresca ou ensilada e silagem de milho

    Directory of Open Access Journals (Sweden)

    Gustavo Chamon de Castro Menezes

    2011-05-01

    Full Text Available It was evaluated intake, rumen and intestinal digestibility and passage and digestion rates in bovines fed diets constituted of corn silage, crushed sugar cane and given fresh, crushed sugar cane and given after 72 hours of storage, ensiled sugar cane with 1% of calcium oxide and with no treatment and a same concentrate fixed at 1% of body weight. All roughage was corrected to contain 10% of crude protein. It was used five rumen-fistulated bovine with average weight of 240 ± 15 kg, distributed in a 5 × 5 Latin square. Abomasum and total fecal collection and two rumen evacuations were carried out in the morning. Animals fed corn silage based diet presented greater rumen digestibility of the protein and intestinal digestibility of the ether extract, greater intake and passage of dry matter, justifying greater intakes of dry matter and neutral detergent fiber corrected for protein and ash (NDFap. The greatest passage rates in animals fed fresh sugar cane based diet justify greater intakes of dry matter and NDFap in relation to the one observed with sugar cane silage supply. Animal consuming corn silage diets present greater dry matter passage rate and NDFap digestion. Diets with fresh sugar cane, stored or not, favor dry matter passage rate and intake, in relation to ensiled sugar cane. The use of calcium oxide in the ensilage does not improve nutrient digestibility neither passage rate of the diet. Sugar cane stored for 72 hours has digestible traits similar to the ones of fresh sugar cane.Avaliaram-se os consumos, as digestibilidades ruminal e intestinal e as taxas de passagem e de digestão de nutrientes em bovinos alimentados com dietas constituídas de silagem de milho, cana-de-açúcar triturada e fornecida in natura, cana triturada e ofertada após 72 horas de armazenamento, cana-de-açúcar ensilada com 1 % de cal e sem tratamento e um mesmo concentrado fixado em 1% do peso corporal. Todos os volumosos foram corrigidos com ureia

  11. Surto de aflatoxicose em bezerros no Rio Grande do Sul Outbreak of aflatoxicosis in calves in southern Brazil

    Directory of Open Access Journals (Sweden)

    Felipe Pierezan

    2010-05-01

    and rolling. The clinical course, as observed by the owners, was 2-3 days; however many calves in this lot that did not die, remained underdeveloped. Three calves were necropsied. Necropsy findings included firm, light tan livers and marked hydrothorax, ascites and edema of the mesentery, mesocolon and of the mucosal folds of the abomasum. Main histopathological changes were restricted to the liver and consisted of fibrosis, moderate megalocytosis, biliary duct hyperplasia and veno-occlusive disease. The search for Senecio spp. contamination in the alfalfa hay resulted negative. The analysis by thin layer chromatography of the corn fed to calves revealed 5,136 ppb of aflatoxin B1. A diagnosis of aflatoxicosis was made based on the characteristic clinical signs and pathology, on the absence of Senecio spp. in the food and on the presence of high levels of aflatoxin in the corn fed to the calves.

  12. Mixing sainfoin and lucerne to improve the feed value of legumes fed to sheep by the effect of condensed tannins.

    Science.gov (United States)

    Aufrère, J; Dudilieu, M; Andueza, D; Poncet, C; Baumont, R

    2013-01-01

    The aim of this study was to investigate whether the use of sainfoin-based condensed tannins (CT) enhances feed value when given with tannin-free legumes (lucerne) to sheep. The experiments were conducted with fresh sainfoin and lucerne harvested at two stages (vegetative stage as compared with early flowering) in the first growth cycle. Fresh sainfoin and lucerne forages were combined in ratios of 100 : 0, 75 : 25, 25 : 75 and 0 : 100 (denoted S100, S75, S25 and S0, respectively). Voluntary intake, organic matter digestibility (OMD) and nitrogen (N) retention were measured in sheep fed the different sainfoin and lucerne mixtures. Loss of dry matter (DM) and N from polyester bags suspended in the rumen, abomasum and small intestine (SI) was also measured using rumen-fistulated sheep and intestinally fistulated sheep. The CT content in sainfoin (S100) decreased with increasing percentage of lucerne in the mixture (mean value from 58 g/kg DM for S100 to 18 g/kg DM for S25) and with growth stage (S100: 64 to 52 g/kg DM). OMD did not differ between different sainfoin/lucerne mixture ratios. Sainfoin and lucerne had an associative effect (significant quadratic contrast) on voluntary intake, N intake, total-tract N digestibility, N in faeces and urine (g/g N intake) and N retained (g/g N intake). Compared with lucerne mixtures (S0 and S25), high-sainfoin-content mixtures (S100 and S75) increased the in situ estimates of forage N escaping from the rumen (from 0.162, 0.188 for S0 and S25 to 0.257, 0.287 for S75 and S100) but decreased forage N intestinal digestibility (from 0.496, 0.446 for S0 and S25 to 0.469, 0.335 for S75 and S100). The amount of forage N disappearing from the bags in the SI (per g forage N) was the highest for high-sainfoin mixtures (from 0.082, 0.108 for S100 and S75 to 0.056, 0.058 for S25 and S0, P < 0.001). Rumen juice total N (tN) and ammonia N (NH3-N) values were the lowest in the high-sainfoin diet (mean tN 0.166 mg/g in S100 as compared with 0

  13. Epithelial, metabolic and innate immunity transcriptomic signatures differentiating the rumen from other sheep and mammalian gastrointestinal tract tissues.

    Science.gov (United States)

    Xiang, Ruidong; Oddy, Victor Hutton; Archibald, Alan L; Vercoe, Phillip E; Dalrymple, Brian P

    2016-01-01

    between the species and different between the rumen and colon in sheep. The importance of nitrogen and iodine recycling in sheep was highlighted by the highly preferential expression of SLC14A1-urea (rumen), RHBG-ammonia (intestines) and SLC5A5-iodine (abomasum). The gene encoding a poorly characterized member of the maltase-glucoamylase family (MGAM2), predicted to play a role in the degradation of starch or glycogen, was highly expressed in the small and large intestines. Discussion. The rumen appears to be a specialised stratified cornified epithelium, probably derived from the oesophagus, which has gained some liver-like and other specialized metabolic functions, but probably not by expression of pre-existing colon metabolic programs. Changes in gene transcription downstream of the rumen also appear have occurred as a consequence of the evolution of the rumen and its effect on nutrient composition flowing down the GIT.

  14. Intoxicação espontânea por Baccharis coridifolia (Compositae em ovinos Spontaneous Baccharis coridifolia (Compositae poisoning in sheep

    Directory of Open Access Journals (Sweden)

    Daniela B. Rozza

    2006-03-01

    , dry feces, sternal ou lateral decumbency, struggling movements, coma and death. Clinical evolution was from 5 to 48 hours. Other sheep that were already before on the pasture were not affected. Main necropsy findings were in the gastrointestinal tube, with wall and serosal oedema, mainly in the reticulo-ruminal fold and of the abomasum, reddening and detachment of the mucosa of forestomachs, and intense hemorrhage in the submucosa. Histopathological findings were degeneration and necrosis of the epithelium of the forestomachs with polymorphonuclear infiltration associated with bacterial colonies, congestion, hemorrhage and oedema, and lymphoid tissue necrosis.

  15. Consumo e dinâmica ruminal da fibra em detergente neutro em bovinos em pastejo no período das águas recebendo suplementação com nitrogênio não-proteico e/ou proteína verdadeira Intake and rumen dynamics of neutral detergent fiber in grazing cattle supplemented with non-protein nitrogen and, or true protein during the rainy season

    Directory of Open Access Journals (Sweden)

    Viviane Aparecida Carli Costa

    2011-12-01

    during rainy season. Five crossbred Holstein × Zebu steers, averaging 335±35 kg of body weight and fitted with rumen and abomasum canullaes were used. The treatments were: control (only pasture, and supplements based on urea, 2/3 of nitrogenous compounds from urea and 1/3 of nitrogenous compounds from albumin, 1/3 of nitrogenous compounds from urea and 2/3 of nitrogenous compounds from albumin, and albumin. Two hundred grams/d of crude protein (CP were supplied from supplements. The experiment was carried out according to a 5 × 5 Latin square design, with five 15-day experimental periods. There were no effects of supplementation on voluntary intake, except for CP intake, which was increased by supplementation. The replacement of urea by albumin in the supplements caused linear effect on the CP intake. The intakes of the other diet components were not affected by the supplement composition. There was no effect on ruminal rate of passage of fibrous compounds. Supplementation increased the estimates of common rate of lag and degradation of NDF. However, no effect of supplement composition alteration was observed on this parameter. Supplementation of cattle with rumen degradable (protein or non-protein nitrogenous compounds for grazing cattle during rainy season does not affect voluntary intake of pasture.

  16. 蒙古绵羊Ghrelin基因的克隆和原核表达%The cDNA Cloning and Prokaryotic Expression of Mongolia Sheep Ghrelin Gene

    Institute of Scientific and Technical Information of China (English)

    梁建荣; 曹贵方; 赵鹏伟; 温世勇; 程兰玲; 凃勇

    2011-01-01

    To construct Mongolia sheep Ghrelin prokaryotic expression vector, and observe its expression in host Escherichia coli strain BL2KDE3), the cDNA of Ghrelin gene was amplified from abomasum fundic gland mRNA of Mongolia sheep by RT-PCR. PCR product was cloned into the T vector pMD-19T to construct pMD-19T-Ghrelin for sequencing. Then the cDNA was subcloned into the prokaryotic expressing plasmid vector pET-32a( + ) and transformed into host Escherichia coli strain BL21(DE3)for expression. The clone was induced by IPTG and was identified by SDS-PAGE and Western-blot. Comparing with the Ghrelin sequence of sheep reported in GenBank, there were two nucleotide differences, but it did not affect the amino acid sequence; the expression product was observed with soluble protein; The result of Western-blot showed that the recombinant protein was recognized by His-antibody specifically. The nucleotide sequence isolated from the recombinant plasmid pET-32a-Ghrelin was the same as expected; the Ghrelin protein was expressed successfully in Escherichia coli, which provided a basis for further investigation of Ghrelin function.%为了克隆蒙古绵羊Ghrelin基因,构建其原核表达载体,并检测其在大肠杆菌中的表达情况.本研究用RT-PCR方法从蒙古绵羊胃组织mRNA扩增获得Ghrelin基因的cDNA序列,克隆到pMD-19T载体后进行序列分析.将测序正确的cDNA序列与原核表达载体pET-32a(+)连接,并转化BL21( DE3)大肠杆菌.经IPTG诱导后进行SDS-PAGE和Western-blot分析.结果表明克隆的蒙古绵羊Ghrelin基因序列与已发表基因序列有2个碱基的差异,该碱基的变化并不影响氨基酸序列,目的蛋白主要以可溶性形式存在,Western-blot初步证实了所获得的融合蛋白是特异的.本研究成功构建了蒙古绵羊Ghrelin基因的原核表达载体,并在大肠杆菌中获得高效表达,为进一步研究蒙古绵羊Ghrelin基因的功能提供参考.

  17. Effect of fall-grazed sericea lespedeza (Lespedeza cuneata) on gastrointestinal nematode infections of growing goats.

    Science.gov (United States)

    Mechineni, A; Kommuru, D S; Gujja, S; Mosjidis, J A; Miller, J E; Burke, J M; Ramsay, A; Mueller-Harvey, I; Kannan, G; Lee, J H; Kouakou, B; Terrill, T H

    2014-08-29

    High prevalence of anthelmintic-resistant gastrointestinal nematodes (GIN) in goats has increased pressure to find effective, alternative non-synthetic control methods, one of which is adding forage of the high condensed tannin (CT) legume sericea lespedeza (SL; Lespedeza cuneata) to the animal's diet. Previous work has demonstrated good efficacy of dried SL (hay, pellets) against small ruminant GIN, but information is lacking on consumption of fresh SL, particularly during the late summer-autumn period in the southern USA when perennial warm-season grass pastures are often low in quality. A study was designed to determine the effects of autumn (September-November) consumption of fresh SL forage, grass pasture (predominantly bermudagrass, BG; Cynodon dactylon), or a combination of SL+BG forage by young goats [intact male Spanish kids, 9 months old (20.7 ± 1.1 kg), n = 10/treatment group] on their GIN infection status. Three forage paddocks (0.40 ha) were set up at the Fort Valley State University Agricultural Research Station (Fort Valley, GA) for an 8-week trial. The goats in each paddock were supplemented with a commercial feed pellet at 0.45 kg/head/d for the first 4 weeks of the trial, and 0.27 kg/head/d for the final 4 weeks. Forage samples taken at the start of the trial were analyzed for crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) content, and a separate set of SL samples was analyzed for CT in leaves, stems, and whole plant using the benzyl mercaptan thiolysis method. Animal weights were taken at the start and end of the trial, and fecal and blood samples were collected weekly for determination of fecal egg counts (FEC) and packed cell volume (PCV), respectively. Adult GIN was recovered from the abomasum and small intestines of all goats at the end of the experiment for counting and speciation. The CP levels were highest for SL forage, intermediate for SL+BG, and lowest for BG forage samples, while NDF and ADF values

  18. Evaluation of propylene glycol and glycerol infusions as treatments for ketosis in dairy cows.

    Science.gov (United States)

    Piantoni, P; Allen, M S

    2015-08-01

    To evaluate propylene glycol (PG) and glycerol (G) as potential treatments for ketosis, we conducted 2 experiments lasting 4 d each in which cows received one bolus infusion per day. Blood was collected before infusion, over 240min postinfusion, as well as 24 h postinfusion. Experiment 1 used 6 ruminally cannulated cows (26±7 d in milk) randomly assigned to 300-mL infusions of PG or G (both ≥99.5% pure) in a crossover design experiment with 2 periods. Within each period, cows were assigned randomly to infusion site sequence: abomasum (A)-cranial reticulorumen (R) or the reverse, R-A. Glucose precursors were infused into the R to simulate drenching and the A to prevent metabolism by ruminal microbes. Glycerol infused in the A increased plasma glucose concentration the most (15.8mg/dL), followed by PG infused in the R (12.6mg/dL), PG infused in the A (9.11mg/dL), and G infused in the R (7.3mg/dL). Infusion of PG into the R increased plasma insulin and insulin area under the curve (AUC) the most compared with all other treatments (7.88 vs. 2.13μIU/mL and 321 vs. 31.9min×μIU/mL, respectively). Overall, PG decreased plasma BHBA concentration after infusion (-6.46 vs. -4.55mg/dL) and increased BHBA AUC (-1,055 vs. -558min ×mg/dL) compared with G. Plasma NEFA responses were not different among treatments. Experiment 2 used 8 ruminally cannulated cows (22±5 d in milk) randomly assigned to treatment sequence in a Latin square design experiment balanced for carryover effects. Treatments were 300mL of PG, 300mL of G, 600mL of G (2G), and 300mL of PG + 300mL of G (GPG), all infused into the R. Treatment contrasts compared PG with each treatment containing glycerol (G, 2G, and GPG). Propylene glycol increased plasma glucose (14.0 vs. 5.35mg/dL) and insulin (7.59 vs. 1.11μIU/mL) concentrations compared with G, but only tended to increase glucose and insulin concentrations compared with 2G. Propylene glycol increased AUC for glucose (1,444 vs. 94.3mg/dL) and insulin (326

  19. Peso e rendimento dos componentes do peso vivo de cordeiros terminados em confinamento com dietas contendo proporções crescentes de resíduo úmido de cervejaria Weight and yield of liveweight components of feedlot finish lambs fed diets with increasing proportions of wet brewery residue

    Directory of Open Access Journals (Sweden)

    M.A. Brochier

    2008-10-01

    mix and wet brewery residue, and its proportions varied in accordance with the treatments. There was a linear decrease in weights of blood, legs, head, liver, lungs, and spleen, and liver proportion, with the increase of the proportion of wet brewery residue on the diets. The proportion, in relation to live weight, of rumen/reticulum and of gastrointestinal tract with content, and the gastrointestinal tract content showed a linear increase; while the weights of rumen/reticulum, abomasum, small intestine, and gastrointestinal tract without content showed a linear decrease with the increase of wet brewery residue in the diets. A quadratic effect was observed in the proportion of rumen/reticulum without content.

  20. 妊娠黑麂(Muntiacus crinifrons)大体解剖分析%Gross anatomy of pregnant black muntjac (Muntiacus crinifrons)

    Institute of Scientific and Technical Information of China (English)

    唐鑫生; 秦文; 任育旺; 汪新建; 李雪峰

    2014-01-01

    为黑麂的保护和人工繁殖提供生物学资料,对一只因盗猎致死的妊娠黑麂进行了解剖。其特点是:上腭有15排腭褶;上颌右侧犬齿脱落,前臼齿和臼齿的齿冠磨损较严重,齿式为0·1·3·3/3·1·3·3=34;瘤胃、网胃、瓣胃、皱胃容积分别为71.6%、9.4%、11.4%、7.6%;小肠7248 mm、大肠5269 mm,分别是体长的6.9倍和5.0倍;肝3叶,重598.5 g,无胆囊;左肺3叶,右肺4叶,重474.4 g;心脏重184 g,占身体总重量的0.8%;双角子宫,胚胎重98.5 g,长14.8 mm,胚体弯曲呈“C”形,眼泡明显,鼻窝、耳泡、鳃弓、肢芽出现。%Gross anatomy was carried out on a pregnant black muntjac (Muntiacus crinifrons), which was found dead due to illegal hunting .The anatomy intends to provide first-hand biological data for the protection and artificial propagation of this species .The ana-tomical features of this subject are 15 rows of rugae palatinae found in the mandible;the incisor on the right side of maxillary is gone and the crown of premolar and molar is heavily worn out , featuring a dental formula of 0· 1· 3· 3/3· 1· 3· 3=34;the capacities of rumen, reticulum, omasum, and abomasums are 71.6%, 9.4%, 11.4%, and 7.6% respectively; the small intestine measures 7248 mm in contrast to 5269 mm of the large intestine , 6.9 times and 5.0 times longer than its body length;3 hepatic lopes are found , weighing 598.5 g, no gall bladder;3 left lung lobes and 4 right lung lobes are found , weighing 474.4 g in total;the heart weighs 184 g, accounting for 0.8%of the total body weight; inside the uterus bicornis , an embryo weighing 98.5 g is found.It measures 14.8 mm long and the embryo proper presents “C” shape, with distinctive eye vesicle and noticeable nasal placode , ear vesicle, gill arch, and limb-bud.

  1. Doenças do sistema digestório de caprinos e ovinos no semiárido do Brasil Diseases of the digestive system of sheep and goats in the semiarid region of Brazil

    Directory of Open Access Journals (Sweden)

    Milena A. Aragão de Lira

    2013-02-01

    Federal University of Campina Grande, Patos, Paraíba, from January 2000 to December 2011. The records were reviewed to determine the occurrence, epidemiology, and the main clinicopathological features of the gastrointestinal diseases diagnosed during the studied period. Out of a total of 512 cases (23.9% of gastrointestinal disorders in small ruminants, 367 (71.7% occurred in goats and 145 (28.1% in sheep. Gastrointestinal helminthiasis and coccidiosis were the most frequent diseases (330 cases. The disorders of the rumen and reticulum (acidosis, simple indigestion, bloat, and ruminal compaction constituted 94 cases. The abomasum was affected by primary and secondary ulcers, and obstruction and compression of the gastrointestinal tract were also observed. Malformations, such as anal atresia and cleft palate were recorded in both species, the latter being associated with ingestion of Mimosa tenuiflora. Among the infectious diseases, five outbreaks of contagious ecthyma, two cases of paratuberculosis, and two cases of gastrointestinal pythiosis were observed. Suspected seven cases of enterotoxemia and nonspecific enteritis were identified. The lack of an integrated control of parasites and the use of inadequate food during the period of lack of forage contributes to the occurrence of a great number of gastrointestinal diseases in small ruminants in the studied area. The practice of conservation of fodder could substantially reduce the occurrence of digestive disorders in the semiarid region.

  2. Conidiobolomicose em ovinos no Estado de Mato Grosso Conidiobolomycosis in sheep in the state of Mato Grosso, Brazil

    Directory of Open Access Journals (Sweden)

    Fabiana M. Boabaid

    2008-01-01

    noisy breathing with dyspnea, and mucous or serohemorrhagic nasal discharge. In three sheep there was unilateral exophthalmia, keratitis and corneal ulceration. A firm whitish, multi-lobulated, friable growth was evident in the ethmoturbinate region at the coronal sections of the head from six affected sheep. In all sheep the choana had similar nodular infiltration which resulted in local obstruction. In three of those there was exophthalmia. There was infiltration in the cribiform plate and brain (2 cases, regional lymph nodes (2, lungs (3, and abomasums (1. Microscopic findings were granulomatous inflammation of the rhinoetimoidal region, with necrosis, lymphocytic infiltration, epithelioid multinucleated giant cells and fibrovascular tissue, surrounding Splendore-Hoeppli material wich contained unstained ghost images of hyphae. The methenamine-silver stain uncover fungi hyphae, rarely ramified with bulbous dilatation in their extremities. Conidiobolus sp. was isolated from nasal tissue lesions of four sheep.

  3. Epithelial, metabolic and innate immunity transcriptomic signatures differentiating the rumen from other sheep and mammalian gastrointestinal tract tissues

    Directory of Open Access Journals (Sweden)

    Ruidong Xiang

    2016-03-01

    different between the species and different between the rumen and colon in sheep. The importance of nitrogen and iodine recycling in sheep was highlighted by the highly preferential expression of SLC14A1-urea (rumen, RHBG-ammonia (intestines and SLC5A5-iodine (abomasum. The gene encoding a poorly characterized member of the maltase-glucoamylase family (MGAM2, predicted to play a role in the degradation of starch or glycogen, was highly expressed in the small and large intestines. Discussion. The rumen appears to be a specialised stratified cornified epithelium, probably derived from the oesophagus, which has gained some liver-like and other specialized metabolic functions, but probably not by expression of pre-existing colon metabolic programs. Changes in gene transcription downstream of the rumen also appear have occurred as a consequence of the evolution of the rumen and its effect on nutrient composition flowing down the GIT.

  4. 转基因豆粕对崂山奶山羊生长性能、肌肉营养成分及组织器官中外源基因转移的影响%Effects of Genetically Modified Soybean Meal on Growth Performance, Meat Nutrient Composition, and Exogenous Gene Transformation to Tissues and Organs of Laoshan Dairy Goats

    Institute of Scientific and Technical Information of China (English)

    刘斌; 秦志华; 黄娟; 张廷荣; 刘文达; 王述柏

    2014-01-01

    In order to analyze the effects of genetically modified soybean meal on growth performance, meat nutrient composition, and exogenous gene transformation to tissues and organs of Laoshan dairy goats, thirty 50-day-old Laoshan dairy goats were randomly divided into two groups with three replicates in each group and five goats per replicate. Goats in different groups were fed concentrates containing non-genetically modified soybean meal and genetically modified soybean meal ( MON89788 and GTS40-3-2 ) , respectively, and were fed the same roughage ( Chinese wildrye) . The experiment lasted for 150 days. The results showed as fol-lows: average daily feed intake, average daily gain and feed to gain ratio in the two groups were not signifi-cantly different (P>0. 05); the contents of dry matter, ether extract, crude protein, ash, calcium and phos-phorus in muscle of dairy goats were not significantly different either ( P >0 . 05 ) . Moreover, no gene rag-ments of genetically modified soybean meal were detected from muscle, liver, pancreas, kidney, spleen, thy-mus, lung, heart, abomasum and small intestine of dairy goats by real-time quantification PCR. The results suggest that genetically modified soybean meal ( MON89788 and GTS40-3-2 ) had no significant effects on growth performance and muscle quality of Laoshan dairy goats, and no genetic horizontal transformation is ob-served in tissues and organs.%本试验旨在研究转基因豆粕对崂山奶山羊生长性能、肌肉营养成分及组织器官中外源基因转移的影响。选取30只50日龄崂山奶山羊随机分为2组,每组3个重复,每个重复5只。精饲料分别以非转基因豆粕和转基因豆粕( MON89788和GTS40-3-2)配制,粗饲料均为干草。试验期150 d。结果表明:2组奶山羊平均日采食量、平均日增重和料重比均无显著差异( P >0.05);奶山羊肌肉的干物质、粗脂肪、粗蛋白质、粗灰分、钙、磷含量2组间亦均无显著差异( P>0

  5. Rendimento dos componentes não-carcaça de cordeiros alimentados com silagem de milho ou cana-de-açúcar e dois níveis de concentrado Non-carcass components yield of lambs fed corn silage or sugar cane under two levels of concentrate

    Directory of Open Access Journals (Sweden)

    Greicy Mitzi Bezerra Moreno

    2011-12-01

    with corn silage or sugarcane under two levels of concentrate. Thirty-two non-castrated Ile de France lambs fed on corn silage or sugar cane with two roughage:concentrate ratios: 60:40 or 40:60. Lambs were confined until they reached 32 kg of body weight (BW, when they were slaughtered. After the bleeding, all non-carcass components (blood, skin, head, feet, rumen, reticulum, omasum, abomasum, small intestine, large intestine, spleen, liver, heart, lung with trachea, pancreas, kidneys with perirenal fat, omental and mesenteric fat were separated and weighed, and their percentages were calculated in relation to the BW. The content of gastrointestinal tract was greater in lambs which fed on sugar cane (15.17% and 60% of roughage (14.55%; while omental and kidney fats were greater in lambs that received corn silage, 0.53 and 0.63%, respectively. The roughage:concentrate ratio and type of forage affected the proportions of rumen, omasum and small intestine in relation to the total weight of gastrointestinal tract. The roughage:concentrate ratio and type of forage influence the content of the gastrointestinal tract of lambs, indirectly affecting the carcass yields. The type of food has greater influence on the proportions of the organs responsible for digestion and absorption of nutrients. The factors that influence the non-carcass components of lambs are varied and contradictory, requiring more research that might encourage their use and, consequently, add more value to the production systems of sheep meat.

  6. Fermentação ruminal e eficiência microbiana em bezerros holandeses alimentados com dietas contendo diferentes níveis de concentrado Ruminal fermentation and microbial efficiency in Holstein calves fed diets with different concentrate levels

    Directory of Open Access Journals (Sweden)

    Peter Johann Bürger

    2000-02-01

    Full Text Available RESUMO - Este trabalho foi realizado para avaliar os efeitos de diferentes níveis de concentrado sobre os parâmetros da fermentação ruminal, as digestibilidades aparente, total, ruminal e intestinal de N, a eficiência microbiana e o balanço de N. Cinco bezerros holandeses, inteiros, fistulados no rúmen e abomaso, com idade média inicial de 5,8±0,7 meses e 107,4±11,0 kg PV médio inicial, foram distribuídos em quadrado latino 5x5 (tratamento x período. Os animais foram alojados em baias individuais e alimentados à vontade com dietas contendo 30,0; 45,0; 60;0; 75,0; e 90,0% de concentrado, com base na MS, em rações contendo como volumoso, o feno de capim coast-cross e no concentrado, o farelo de soja, fubá de milho. Os valores de pH foram influenciados pelos níveis de concentrado das rações, e , 11,3 horas após a alimentação, foram estimandos os valores mínimos de 6,10; 5,89; 5,67; 5,46; e 5,24, para as rações com níveis de 30,0; 45,0; 60,0; 75,0; e 90,0%de concentrado nas rações, respectivamente. A concentração de amônia ruminal reduziu linearmente, em função dos tempos pós-alimentação, apresentando comportamento quadrático, com valores mínimos de 6,84; 7,14; 7,63; 7,82; 8,09; e 8,00 mg/dL, para 86,31; 84,86; 83,41; 81,95; 77,59; e 68,86% de concentrado nas rações. O numero de protozoários ruminais reduziu linearmente com o aumento dos níveis de concentrado nas rações. A eficiência de síntese de compostos nitrogenados microbianos aumentou linearmente com os níveis de concentrado nas rações.ABSTRACT - This work was conducted to evaluate the effects of different concentrate levels on the parameters of at ruminal fermentation, the apparent, total, ruminal and intestinal N digestibilities, the microbial efficiency and the N balance. Five rumen and abomasum fistulated bull Holstein calves, with an initial average age of 5.8±0.7 months and initial average of 107.4±11.0 kg LW were allotted to a 5x5

  7. Consumo e digestibilidade em bovinos em pastejo durante o período das águas sob suplementação com fontes de compostos nitrogenados e de carboidratos Intake and digestibility in cattle under grazing during rainy season and supplemented with different sources of nitrogenous compounds and carbohydrates

    Directory of Open Access Journals (Sweden)

    Viviane Aparecida Carli Costa

    2011-08-01

    suplemento.The objective of this work was to evaluate the effects of different chemical forms of nitrogenous compounds (protein and non-protein and carbohydrates (starch and soluble fiber on intake, digestibility, and ruminal synthesis of microbial protein in cattle supplemented during rainy season. It was used five crossbred Holstein × Zebu steers, with average initial body weight of 211 ± 35 kg and fistulated in the rumen and abomasum. The treatments were: control (only pasture, and supplements based on corn + soybean meal; corn + urea, citrus pulp + soybean meal, and citrus pulp + urea. The supplements were balanced to present 30% of crude protein (CP, on dry matter (DM basis, and provided at 3 g/kg BW. The experiment was carried out according to a 5 × 5 Latin square design in a 2 × 2 + 1 factorial arrangement, composed of two sources of nitrogenous compounds, two sources of carbohydrates and a control treatment. Pasture intake was reduced when supplements were fed to animals, with average substitution coefficient of 2.11 g of DM of pasture/g of DM of supplement. Supplementation did not alter the total and ruminal digestibility coefficients of DM and the dietary content of total digestible nutrients (TDN neither. Supplemented animals presented higher ruminal and total digestibility coefficients of CP than non-supplemented animals. The efficiency of microbial protein synthesis (EMPS, average of 123.1g of microbial CP/kg, was not changed by supplementation. However, animals supplemented with corn presented higher EMPS in comparison to animals supplemented with citrus pulp (137.6 and 106.1 g microbial CP/kg of TDN, respectively. Protein-energy supplementation for cattle grazing in tropical pastures during rainy season does not cause nutritional benefits, which reflects the high coefficient of substitution of forage by the supplement.

  8. Parâmetros nutricionais e produtivos em bovinos de corte a pasto alimentados com diferentes quantidades de suplemento Nutritional and productive parameters of beef cattle on pasture fed different amounts of supplement

    Directory of Open Access Journals (Sweden)

    Polyana Albino Silva Machado

    2011-06-01

    supplement on the intake, digestibility, body weight gain and microbial synthesis efficiency in beef cattle. Four Holstein-Zebu steers fistulated in the esophagus, rumen and abomasum with an average of 250 kg of body weight were used in experiment 1. The supplement was made up cottonseed meal, ground corn grain, mineral mixture and urea/ammonium sulfate. The diets were: control (salt and supplements levels of 0.33, 0.66 and 1.00% of body weight (BW. Maximum intake of dry matter (DM, neutral detergent fiber (NDF and total digestible nutrients (TDN were estimated at 1.09, 0.73 and 1.20% of BW, respectively. It was observed a linear intake increase to organic matter (OM, crude protein (CP, ether extract (EE and non-fiber carbohydrates (NFC intake as supplement offer increased. The total digestibility from DM, OM, CP, EE and NDF increased linearly with the intake of supplement but no affected on microbial efficiency. Nineteen Zebu steers aging 18 months and mean body weight of 320 kg were used in experiment 2. The diets were: maintenance, control (salt, and the daily supplement offer of 1, 2 and 3 kg. It was observed linear increase in DM, OM, CP, EE, NDF and NFC intake as supplement offer increased. Maximum intake of TDN, diet TDN content and average daily gain (ADG were estimated to occur as 2.88, 2.62 and 2.69 kg of supplement offer, respectively. A quadratic effect was observed for total digestibility in all diet components. The microbial efficiency was not influenced by diet supplementation. The neutral detergent fiber digestibility is highest for a intake of approximately 1.5 kg of the supplement/day, which results in the best response to the ADG obtained with the intake of 400 g of CP.

  9. RUMINAL DEGRADATION OF THE DRY MATTER, PROTEIN AND THE AMINOACIDS OF THE CORN AND CORN GLUTENS MEAL DEGRADAÇÃO RUMINAL DA MATÉRIA SECA, DA PROTEÍNA E DOS AMINOÁCIDOS DO MILHO E DE GERMENS DE MILHO

    Directory of Open Access Journals (Sweden)

    Juliana Borbari Dourado

    2007-09-01

    Full Text Available

    The present work was carried out at the Faculty of Agriculture and Animal Science, UNESP-Jaboticabal. Three ¾ Holstein x Zebu bovines fistulated on rumen, abomasum and intestine using a fixed cannula type T were used. Four types of feed: corn (M, 1% EE corn gluten meal (G1, 7% EE corn gluten meal and 10% EE corn gluten meal (G10 were tested.  All feed were also extruded: CornEx, G1Ex, G7Ex and G10EX. Ruminal incubation was done using the nylon bags technique, and degradability of dry matter, crude protein and disponibilization of amino acids were analyzed. Basic diet was composed of corn gluten meal, soybean meal and hay at roughage (70: concentrate (30 ratio. Ruminal degrading of dry matter feed M, G1, G7 and G10 presented the following values: 37.5%, 56.7%, 56.8% and 55.1% respectively.  Extruded feed: 52.3%, 68.9%; 69.0% and 61.6% for Mex, G1Ex, G7Ex and G10Ex. The corn was significantly inferior to others and extruded feed increased ruminal degradedly for all feed. The degradability of crude protein was 27.0%, 60.9%, 56.8% and 35.1% for M, G1, G7 and G10; 50.8, 52.2, 66.4 and 59.6 for MEx; G1Ex; G7Ex and G10Ex, respectively. Statistically the G1 feed was equal to M and both were inferior to G7 and G10. The extrusion process did not increase the degradability of crude protein of G1. The lysine presented high ruminal degradability for tested feed, showing values above 99%. The metionin presented values of degradability inferior for M (37.4% related to G1 (57.1%.  The treonine did not present differences between feed and extrusion. It can be concluded that the processing of corn to obtain by-products can increase the ruminal degradability of dry matter, protein and metionine, but depending on intensity of processing, the degradability of crude protein may not be altered when compared to corn. The extrusion increased the ruminal degradability of dry matter of corn and by-products.

    KEY-WORDS: Amino acids, degradability, extrusion

  10. Uréia em suplementos protéico-energéticos para bovinos de corte durante o período da seca: características nutricionais e ruminais Urea in protein-energy supplements for beef cattle during the dry season: nutritional and ruminal traits

    Directory of Open Access Journals (Sweden)

    Eduardo Henrique Bevitori Kling de Moraes

    2009-04-01

    Full Text Available Objetivou-se avaliar o consumo, a digestibilidade, o pH e a concentração de amônia do líquido ruminal, a concentração de nitrogênio sérico e urinário e a síntese microbiana em novilhos recebendo suplemento durante o período da seca. Utilizaram-se quatro novilhos mestiços Zebu × Holandês, castrados, com peso médio inicial de 430 kg, fistulados no esôfago, rúmen e abomaso e distribuídos em quatro piquetes de Brachiaria decumbens, cada um com 0,40 ha. Cada animal recebeu 4,0 kg/dia de suplemento, constituído de milho moído, farelo de algodão, mistura mineral e uréia/sulfato de amônia em quatro níveis (0; 1,2; 2,4 e 3,6% na matéria natural. Os consumos de matéria seca total, 2,0% (10,9 kg/dia, e de pasto, 2,3% (7,1 kg/dia, matéria orgânica, 2,2% (9,7 kg/dia, matéria orgânica do pasto, 2,3% (6,3 kg/dia, proteína bruta, 1,9% (1,3 kg/dia, extrato etéreo, 3,6% (0,4 kg/dia, fibra em detergente neutro, 1,9% (5,8 kg/dia, carboidratos totais, 2,3% (8,2 kg/dia, e carboidratos não-fibrosos, 2,9% (4,1 kg/dia, foram influenciados de forma quadrática pelos níveis de uréia nos suplementos. Apenas a digestibilidade total da matéria orgânica foi afetada, de forma linear positiva, pelos níveis de uréia no suplemento. Não foram observados efeitos dos níveis de uréia no suplemento sobre o pH ruminal, entretanto as concentrações de amônia aumentaram linearmente de acordo com os níveis de uréia, ocasionando aumento linear na excreção de nitrogênio sérico e urinário. A eficiência microbiana, em qualquer forma de expressão, não diferiu entre os níveis de uréia.Four Zebu × Holstein steers were fistulated in rumen, abomasums and esophagus, with 430 kg of live weight grazing in four paddocks of Brachiaria decumbens (.40 ha, feed supplements were used to evaluate intake, digestibility, pH and ammonia ruminal concentration, nitrogen in serum and urine and microbial yield. The animals received 4.0 kg/animal/day of

  11. Órgãos internos e trato gastrintestinal de novilhos de gerações avançadas do cruzamento rotativo entre as raças Charolês e Nelore terminados em confinamento Internal organs and gastrointestinal tract of feedlot finished steers of advanced generations of rotational crossbreeding between Charolais and Nellore

    Directory of Open Access Journals (Sweden)

    Luís Fernando Glasenapp de Menezes

    2007-02-01

    and gastrointestinal tract (GIT of feedlot purebred (Charolais C and Nellore N and crossbred steers from second (G2 (¾C ¼N and ¾N ¼C, third (G3 (5/8C 3/8N and 5/8N 3/8C and fourth (G4 (11/16C 5/16N and 11/16N 5/16C generations of rotational crossbreeding slaughtered at 23 month of age. Crossbred steers from G2, G3 and G4 had, respectively, 14.95, 17.25 and 18.46% higher empty body weight (EBW than that from purebred animals. A positive heterosis for cold carcass yield expressed as a proportion of EBW (CCYEBW was observed in G2, G3, and G4. Weights of heart, lungs, and kidneys adjusted for EBW were lower on crossbred compared to purebred steers; the heterosis was significant for the weights of heart (-18.29% and kidneys (-14.29% in G3 and for that of lungs (-13.45% in G4. Weights of all organs of the GIT either expressed as 100 kg of EBW or as proportion of slaughter body weight (SBW were lower on crossbred than on purebred steers with the exception of rumen weight in G2. Retained heterosis was positive and significant for EBW, CCYEBW and liver weight. However, it was negative for amount of blood and weights of heart and small plus large intestines when expressed as 100 kg of EBW and SBW. Within purebred animals, N showed greater CCYEBW (61.64% than C (57.09. Conversely, weights of rumen, abomasum, small plus large intestine, and GIT were all higher on C than N steers; excepting for small plus large intestine, the same was observed when weights of the remaining GIT components were expressed as proportion of EBW and SB. These differences partially explain the increased carcass yield on N steers.

  12. Consumo, digestibilidade e parâmetros ruminais em bovinos de corte alimentados com dietas contendo silagem de sorgo e pré-secado de capim-tifton 85 Intake, digestibility and ruminal parameters in beef cattle fed diets with sorghum silage and tifton 85 haylage

    Directory of Open Access Journals (Sweden)

    Odilon Gomes Pereira

    2007-12-01

    digesta, the pH and the ammonia ruminal concentration in beef cattle fed diets containing tifton 85 haylage and sorghum silage in the following proportions: 100:0; 68:32; 34:66 and 0:100, respectively in DM basis. Four crossed animals (H × Z fistulated in the rumen and abomasum, with average body weight of 364 kg were assigned to a 4 × 4 Latin square. The forage: concentrate ratio was 60:40, in DM basis. The daily intake of DM, OM, CP, total carbohydrates (TC, as well as the total apparent digestibility of CP, EE, NFC and NDF were not influenced by the diets, with mean values of 6.01, 5.69, 0.76, and 4.64kg/day and 67.94, 82.12, 84.43, and 53.57%, respectively. The intake of EE, NFC, NDF and TDN and the total apparent digestibility of DM, OM and TC linearly increased with the increment of sorghum silage in the forage. The ruminal and intestinal digestibility of DM, TC, NFC and NDF were not influenced by the diets. The pH was not influenced by the collection time or by the diets. The ammonia concentration was influenced by the collection times, estimating the maximum value of 13.14 mg/100 mL, at 2.90 hour after feeding. Ruminal digesta passage rates of 4.10, 4.22, 4.27, and 5.30%/hour were respectively estimated for diets containing 0, 32, 66, and 100% of sorghum silage in the forage. The level of sorghum silage in the diet did not influence the microbial efficiency. The use of Tifton 85 haylage associated with sorghum silage has shown to be a great alternative of forage for finishing beef cattle.

  13. Intoxicação por Stryphnodendron fissuratum (Mimosoideae em bovinos Poisoning by Stryphnodendron fissuratum (Mimosoideae in cattle

    Directory of Open Access Journals (Sweden)

    Eduardo V. Ferreira

    2009-11-01

    animais, mas não ocorreu fotossensibilização.Epidemiological, clinical and pathological aspects of natural and experimental Stryphnodendron fissuratum poisoning in cattle in Central-Western Brazil were evaluated. The coiled pods of this tree are popularly known as "rosquinha" (small coil; they mature during July-September, when they fall to the ground, and are consumed by cattle causing mortalities. Spontaneous outbreaks of poisoning were investigated in the municipalities of Aruanã, State of Goias, Guiratinga and Rondonópolis State of Mato Grosso, and Rio Verde de Mato Grosso, State of Mato Grosso do Sul. Commonly the poisoning occurred in properties with Nelore cattle herds, which were raised extensively on Brachiaria spp. pastures. The clinical course in natural cases ranged from 24 hours to 10 days, and the morbidity and case fatality rates were 0.9-25% and 15-100%, respectively. The main clinical signs in the spontaneous poisoning were apathy, anorexia, agressiveness, jaundice, sialorrhoea, incoordination, dysmetria, retraction of the abdomen, uneasiness and pasty black feces with stings of mucus or blood, diarrhea, edema of the dewlap and photosensitization. At necropsy, jaundice, edema of the subcutaneous tissue, mainly of the cervical region, hemorrhages of serous membranes, ascitis and hidrotorax, edema of the mesentery, perirenal edema, increased size of liver and kidney, reddening of the ruminal mucosa, and abomasum ulcers were observed. The diagnosis of S. fissuratum poisoning was based on epidemiological, clinical and pathological findings, and in the experimental reproduction by the administration of the pods of this plant to cattle. Experimentally, daily doses of 4 and 20g of pods by kg body weight, respectively, were administered to two bovines. Both animals had digestive signs and died, but photosensitization was not observed.

  14. Effects of Ambroxol on Concentration of Fluconazole in Rat Lung TissueΔ%氨溴索对大鼠肺组织中氟康唑药物浓度的影响Δ

    Institute of Scientific and Technical Information of China (English)

    陈桂林; 翁春梅; 刘广军; 杜贯涛

    2016-01-01

    目的:建立测定大鼠肺组织中氟康唑的高效液相色谱-紫外吸收检测( HPLC-UV)法,探讨氨溴索对大鼠肺组织中氟康唑药物浓度的影响。方法:将实验大鼠按随机数字表法分为A、B组,每组各10只,A组大鼠首日经尾静脉给予72 mg/kg注射用氟康唑,以后给予1日36 mg/kg;B组大鼠先经胃灌注给予6 mg/kg氨溴索,1日2次,上午给予氨溴索30 min后给予注射用氟康唑。连续给药2 d后,第3日给予氟康唑后2 h对大鼠颈静脉取血后解剖,取肺组织备用。采用HPLC-UV法同时测定最后一次使用氟康唑后2 h大鼠的肺组织浓度。结果:氟康唑线性范围均为2.5~80μg/ml之间,回归方程为X=0.052C-0.0276,相关系数r=0.9999。 A组大鼠肺组织氟康唑的浓度(28.1±19.6)μg/ml,B组为(26.6±17.7)μg/ml,2组的差异无统计学意义(P<0.05)。结论:氨溴索对肺组织中的氟康唑药物浓度无显著影响,但尚需结合临床实际行进一步的研究。%OBJECTIVE:To establish a HPLC-UC method of fluconazole and explore effects of ambroxol on concentration of fluconazole in rat lung tissue .METHODS:Rats were divided into group A and group B via the random number table , with 10 cases in each .And group A were treated with 72 mg/kg fluconazole injection by caudal vein in the first day , followed by 36 mg/kg for a day during the latter treatment .Group B received 6 mg/kg ambroxol by abomasum infusion , twice a day , and after the administration of ambroxol for 30 min in the morning , fluconazole injection was given.After administration of 2 d, 2 h after administration of fluconazole in the 3rd day, the blood was drawn from jugular vein of rats and then anatomy was conducted , lung tissue of rats were taken .HPLC-UV method was adopted to determine the concentration of rat lung tissue 2 hours after injecting fluconazole in the last administration . RESULTS:The calibration curve

  15. Formas de utilização do milho em suplementos para novilhos na fase de terminação em pastagem no período das águas: desempenho e parâmetros nutricionais Corn, in different forms, in multiple supplements for finishing crossbred steers on pasture during rainy season: performance and nutrition parameters

    Directory of Open Access Journals (Sweden)

    Marlos Oliveira Porto

    2008-12-01

    season was evaluated. Sixteen crossbred steers with initial weight of 384 kg and 18 mo old were distributed to four supplements mineral mix (MM (control and three multiple supplements with different feeding forms of corn: ground corn (GC; ground ear-corn (GEC and ground whole-plant corn (GWPC - in a completely randomized experimental design with four replicates. The animals were kept in pasture, 6 ha of signal grass, divided into four paddocks of 1.5 ha, with total dry matter and potentially digestible dry matter availabilities of 4.77 t/ha and 3.14 t/ha, respectively. Four esophagus, rumen and abomasum fistulated crossbred animals with initial weight of 390 kg, were used to evaluate the nutritional parameters. Animals were distributed to a 4 × 4 Latin square, composed of four supplements and four experimental periods. The animals in the control group were fed daily with 60 g/day of MM, while the others were fed with 1.0 kg/day of the multiple supplements different. Multiple supplements did not influence the performance of animals, but serum urea concentrations were higher in animals fed with the different multiple supplements. Crude protein and intake and digestibility increased with the use multiple supplements. Animals of the control showed higher dry matter and organic matter intake from the pasture. The different multiple supplements (1 kg/day promoted numerical increase of up to 165 g/animal/day in relation to control during rainy season. Ground whole-plant corn and ground ear-corn are equivalent to ground corn for steers in the finishing phase in pasture in the rainy season.

  16. Aspectos clínico-patológicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos Clinic and pathological and laboratory aspects of experimental poisoning by Bothrops alternatus venom in cattle

    Directory of Open Access Journals (Sweden)

    Saulo A. Caldas

    2008-06-01

    apathy. The laboratory exams revealed progressive normocytic normochromic anemia, thrombocytopenia, reduction in fibrinogen and total plasma proteins, decreased hematocrit and hemoglobin, and slight increase in creatine kinase and lactate dehydrogenase. When subjected to necropsy, the animals that received the venom through the subcutaneous route showed large hematomas and hemorrhagic areas in the subcutaneous tissue, extending from the site of inoculation. The animals in which inoculation was performed intramuscularly exhibited, in addition, intramuscular hemorrhage. The left endocardium showed extensive hemorrhagic lesions, and petechiae were found on the serosae of rumen, omasum, abomasum and gall bladder. The colon, rectum and perirenal areas were surrounded by clotted blood in three animals. Besides hemorrhage, the histological examination revealed hemorrhage and coagulative muscle necrosis in the vicinities of the inoculation site in the animals that received the venom by the intramuscular route. These lesions were mild in the muscles close to the site of inoculation in the animals inoculated by the subcutaneous route. The swelling on the site of inoculation and its surroundings was a consequence of blood accumulation and not merely edema. Myoglobinuria and gross or microscopic lesions were not observed in the kidneys. This study indicates that B. alternatus can lead adult bovines to death if they release all their venom during a bite. Conversely, since venomous ophidians can control the amount of poison they inoculate and they probably do not identify bovines as potential preys, it is likely that the number of accidents involving this species is small, which is in agreement with the findings of the majority of the centers for anatomical-pathological diagnosis in Brazil.

  17. Aspectos epidemiológicos da seneciose na região sul do Rio Grande do Sul Epidemiological aspects of seneciosis in southern Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Fernando Sérgio Castilhos Karam

    2004-12-01

    of Rio Grande do Sul, Brazil. This paper presents epidemiological data of 24 outbreaks in cattle and one in horses, diagnosed by the Regional Diagnostic Laboratory at Pelotas University in southern Rio Grande do Sul, from 1998 to 2000. Additionally, data of 54 outbreaks which occurred in 1978-1997 were analyzed. The farms where outbreaks occurred in 1998-2000 were visited to obtain clinical and epidemiological data and to verify the presence of Senecio spp. Eleven outbreaks (45.83% affected cattle up to 3 years of age, and 13 (54.16% cattle over 3 years. Nine (37.5% outbreaks affected females and 15 (62.5% males. Ten (41.66% outbreaks occurred in spring, 4 (16.66% in summer, 5 (20.83% in autumn, and 5 (20.83% in winter. Morbidity rate was estimated with 4.92% and case fatality with 95.59%. The predomionating Senecio species were S. brasiliensis on 12 farms (57.14%, S. selloi on 10 (47.61%, S. oxyphyllus on 6 (28.57%, S. heterotrichius on 3 (14.28%, and S. leptolobus on 1 farm (4.76%. The main clinical signs were progressive emaciation, incoordination, diarrhea, tenesmus, rectal prolapse and aggressiveness. The clinical manifestation periods of affected cattle observed during the farm visits, or of cattle sent for post-mortem examination,were 24-96 hours in 4 outbreaks (16.66%, 4-7 days in 7 (29.16%, 1-2 weeks in 4 (16.66%, 2-3 weeks in 2 (8.33%, 1-2 months in 2 (8.33%, and 2-3 months in 1 outbreak (4.16%. In 4 outbreaks (16.66% the clinical manifestation period was not established. In outbreaks with longer clinical manifestation periods some animals showed photosensitization. The main necropsy findings were a hard and enlarged liver, distended gall bladder, edema of the mesenterium and abomasum, and increased amount of liquid in the cavities. Histopathological findings were fibroplasia, megalocytosis and biliary ductal proliferation of the liver, and spongy degeneration of the cerebral white matter. Samples of different Senecio species, in different seasons, were