WorldWideScience

Sample records for abnormal cervical cytology

  1. Abnormal cervical cytology and health care use

    DEFF Research Database (Denmark)

    Frederiksen, Maria Eiholm; Baillet, Miguel Vázquez-Prada; Dugué, Pierre-Antoine;

    2015-01-01

    OBJECTIVE: This study aimed to assess the long-term use of health care services in women with abnormal cytology results compared to women with normal cytology results. METHODS: We did a nationwide population-based study, using women aged 23 to 59years participating in the national organized......" the cytology result and for the 5-year period "after" the result. RESULTS: During the "before" period exposed women had more contacts to GPs, more contacts to psychologists/psychiatrist, and more hospital admissions than non-exposed women. In both exposed and non-exposed women, health care use increased from...... the "before" to the "after" period. This increase was significantly higher for exposed than non-exposed women regarding contacts to GP, admissions to hospitals, and drug use. CONCLUSION: Women with abnormal cytology results constitute a selected group with a higher health care use than other women even before...

  2. Histopathological Correlation of Squamous Cell Abnormalities Detected on Cervical Cytology

    Directory of Open Access Journals (Sweden)

    Remzi ABALI

    2011-05-01

    Full Text Available Objective: To investigate the correlation between cytology and cervical biopsy in patients with squamous cell abnormality on cervical cytology.Material and Method: The cervical smears diagnosed in our clinic between 2005-2008 were reviewed retrospectively. Cases which exhibited squamous cell abnormality (n: 374 were evaluated.Results: The mean age was 45.15±10.78. In the cytopathological results, 256 (68.4% ASC-US, 21 (5.6% ASC-H, 31 (8.2% LSIL, 48 (12.8% HSIL, and 8 (4.8% invasive carcinomas were diagnosed. Histopathological results were 213 (57% nonneoplastic, 85 (22.7% CIN I, 14 (3.7% CIN II, 34(9.0% CIN III and 28 (7.5% invasive squamous cell carcinomas. Including all squamous cell abnormalities, the sensitivity of the smear test in CIN I and higher grade lesions was 56.95% and the false positivity was 43.04%. Excluding ASC-US and ASC-H lesions, the sensitivity of the smear test was 77.31% and the false positivity was 22.68%. After evaluating cervical cytohistopathological correlation, the positive predictive value was found to be 100% in invasive carcinoma, 62% in HSIL and 38% in LSIL.Conclusion: As the grade of cytopathological result increases, the correlation between biopsy and the smear test also increases. The high sensitivity of the cervical smear test for high-grade lesions shows that it is an effective screening test.

  3. The management of women with abnormal cervical cytology in pregnancy.

    LENUS (Irish Health Repository)

    Flannelly, Grainne

    2010-02-01

    The management of women with abnormal cytology in pregnancy represents both a diagnostic and a therapeutic challenge for colposcopists. The emphasis should be on diagnosis and confirmation of cervical precancer (Cervical intraepithelial neoplasia (CIN) or Adenocarcinoma in situ (AIS), thus excluding invasive cancer). Following an initial assessment, careful follow-up is essential. This must include colposcopy and take into account the physiological changes of the cervix during pregnancy and the puerperium. The management of women with invasive cancer diagnosed during pregnancy depends on the gestation at diagnosis and requires careful assessment and multidisciplinary planning.

  4. Comparison of Efficacy in Abnormal Cervical Cell Detection between Liquid-based Cytology and Conventional Cytology.

    Science.gov (United States)

    Tanabodee, Jitraporn; Thepsuwan, Kitisak; Karalak, Anant; Laoaree, Orawan; Krachang, Anong; Manmatt, Kittipong; Anontwatanawong, Nualpan

    2015-01-01

    This study was conducted to 1206 women who had cervical cancer screening at Chonburi Cancer Hospital. The spilt-sample study aimed to compare the efficacy of abnormal cervical cells detection between liquid-based cytology (LBC) and conventional cytology (CC). The collection of cervical cells was performed by broom and directly smeared on a glass slide for CC then the rest of specimen was prepared for LBC. All slides were evaluated and classified by The Bethesda System. The results of the two cytological tests were compared to the gold standard. The LBC smear significantly decreased inflammatory cell and thick smear on slides. These two techniques were not difference in detection rate of abnormal cytology and had high cytological diagnostic agreement of 95.7%. The histologic diagnosis of cervical tissue was used as the gold standard in 103 cases. Sensitivity, specificity, positive predictive value, negative predictive value, false positive, false negative and accuracy of LBC at ASC-US cut off were 81.4, 75.0, 70.0, 84.9, 25.0, 18.6 and 77.7%, respectively. CC had higher false positive and false negative than LBC. LBC had shown higher sensitivity, specificity, PPV, NPV and accuracy than CC but no statistical significance. In conclusion, LBC method can improve specimen quality, more sensitive, specific and accurate at ASC-US cut off and as effective as CC in detecting cervical epithelial cell abnormalities. PMID:26514540

  5. Histological Correlation of Glandular Abnormalities in Cervical Liquid-Based Cytology

    Directory of Open Access Journals (Sweden)

    Yosuke Kawakami

    2011-01-01

    Full Text Available Conventional Papanicolaou smear method is still commonly used for cervical cancer screening in Japan, despite the liquid-based cytology (LBC that has become a global tendency in the world recently. One of the obstacles in the way of popularization of this method seems to be the confusion as to diagnosis upon cervical glandular lesions. We performed comparison study between LBC and conventional Papanicolaou smear about cytological diagnosis using split-sample method in 4522 patients. In 13 cases analyses, which were reported with either AGC or adenocarcinoma by either method, LBC tends to be milder than that by conventional smear, however, the credibility of LBC is considered to be near to that of conventional smear with regard to screening for glandular abnormalities. These results indicate that cervical cancer screening should shift to LBC under the enough experience and appropriate dealing with the cytological diagnosis.

  6. Cervical acid phosphatase detection: A guide to abnormal cells in cytology smear screening for cervical cancer

    OpenAIRE

    Deb Prabal; Iyer Venkateswaran; Bhatla Neerja; Markovic O; Verma Kusum

    2008-01-01

    Background: Cervical acid phosphatase-Papanicolaou (CAP-PAP) test has recently been described for detection of acid phosphatase enzyme in abnormal squamous cells, and has been proposed as a biomarker-based technology for the screening of cervical cancer. Materials and Methods: Eighty-one consecutive cervical smears were subjected to routine Papanicolaou (Pap) staining as well as CAP-PAP, which combined cytochemical staining for acid phosphatase with modified Pap stain. Statistical evaluation ...

  7. Correlative analysis between abnormal cervical cytology and pathology of vaginoscopic biopsy or conization

    Institute of Scientific and Technical Information of China (English)

    Li Cai-juan; Lang Jing-he; Cheng Xue-mei; Wang You-fang

    2006-01-01

    Objectives: To evaluate the predictive value of cervical intraepithelial neoplasia (CIN)Ⅲ/ carcinoma in situ (CIS) by correlating analysis between abnormal cervical cytologic findings and pathological diagnosis of vaginoscopic biopsies or conization.Methods: Routine cervical cytology screening was performed in 31,634 cases by fluid-based thin-layer method (ThinPrep cytology test, TCT), 948 patients had both abnormal squamous cell appearance by TCT and pathological diagnosis of vaginoscopic biopsies and /or cervical conization. The predictive value of CINⅢ/CIS were studied retrospectively by correlating analysis of different cytology abnormalities and pathology diagnosis.Results: Cytologically, 1,260 out of 31,634 TCT tests showed abnormal squamous cells appearance, including atypical squamous cell of undetermined significance (ASCUS) 675 cases(2.13%), low squamous intraepithelial lesion (L-SIL) 379 cases(1.20%), high squamous intraepithelial lesion (H-SIL)176 cases(0.56%),cancer 30 cases (0.09%). Among 948 patients with pathological diagnosis, there were CINⅡ-Ⅲ in 70 cases(7.38%) and CINⅢ/CIS in 56 cases.(5.91%). The relative risks (RR) of different precancerous TCT results in predicting CIN Ⅲ/CIS validated by pathology are as follow: AUSCUS 14.7% (95% confidence interval (CI) 8.0-27.0), Lsil 13.9% (95% CI 6.3-30.9), Hsil 126.1 (95% CI 60.6-218.6). The RR of AUSCUS group is not significantly different from that of L-SIL group(P =0.951). However, the RR of CINⅢ/CIS morbidity between the H-SIL or cancer group and the ASCUS or L-SIL group are significantly different (all P value <0.01).Conclusion: Vaginoscopic biopsy could verify pathological CINⅡ-Ⅲ and CINⅢ/CIS from cases with abnormal TCT results. There is significantly greater risk of being CINⅢ/CIS validated by vaginoscopic biopsy in the H-SILpatients,while ASCUS and L-SIL group have the coequal risk.

  8. The use of telemedicine in primary care for women with cervical cytological abnormalities.

    Science.gov (United States)

    Etherington, lan J; Watts, Anne D; Hughes, Elisabeth; Lester, Helen E

    2002-01-01

    Telemedicine can be used in two different ways in the context of the National Health Service Cervical Screening Programme. The first method allows primary health-care providers to offer direct online booking of clinic appointments according to predefined algorithms based on the woman's cytological abnormality. The second method is telecolposcopy, which is designed to be used by nurses in primary care. Preliminary data confirm that such a system can be used reliably to make diagnoses. The technology is easily adaptable for realtime teleconsultation.

  9. Prevalence and molecular epidemiology of human papillomavirus infection in Italian women with cervical cytological abnormalities

    Directory of Open Access Journals (Sweden)

    Angelo Meloni

    2014-03-01

    Full Text Available Background. Human papillomavirus (HPV infection is the most common sexually transmitted infection and high-risk HPV types are a necessary cause for the development of cervical cancer. The present study investigated the HPV-type specific prevalence in 650 women, aged 15-76 years, with cytological abnormalities and the association between HPV infection and cervical disease in a subset of 160 women for whom cytological results for Pap-Test were available, during the period 2008-2011 in Cagliari (Southern Italy.Design and Methods. HPV-DNA extraction was performed by lysis and digestion with proteinase K and it was typed by using the INNOLiPA HPV Genotyping Assay.Results. Overall the HPV prevalence was 52.6%; high-risk genotypes were found in 68.9% of women and multiple-type infection in 36.1% of HPV-positive women. The commonest types were HPV-52 (23.4%, HPV-53 (15.7%, HPV-16 (15.4% and HPV-6 (12.4%. Among the women with cytological diagnosis, any-type of HPV DNA was found in 49.4% of the samples and out of these 93.7% were high-risk genotypes. Genotype HPV 53 was the commonest type among women affected by ASCUS lesions (21.4%, genotype 52 in positive L-SIL cases (22.5%, genotype 16 H-SIL (27.3%.Conclusions. This study confirmed the high prevalence of HPV infection and high-risk genotypes among women with cervical abnormalities while, unlike previously published data, genotype HPV-52 was the most common type in our series. These data may contribute to increase the knowledge of HPV epidemiology and designing adequate vaccination strategies.

  10. HPV genotype prevalence in cervical specimens with abnormal cytology: a report from north-east Italy.

    Science.gov (United States)

    Rassu, Mario; Bertoloni, Giulio; Mengoli, Carlo; Peron, Antonella; Benedetti, Paolo; Palu', Giorgio

    2005-01-01

    We have investigated the prevalence of HPV DNA in cervical samples collected from 1335 women with abnormal Pap test and analysed the degree of association of HPV genotype with cervical cytological abnormality and also with patient age. The study was principally aimed at providing some cross-sectional figures on the epidemiology of HPV in our area, where the ethnic background is expected to rapidly evolve due to extensive immigration from overseas. 471 (35.3%) of the 1335 patients screened were positive for HPV DNA. A clear association was observed between cytological findings and the proportion of patients with positive HPV PCR, namely 24.0% HPV positivity in the ASCUS group (atypical squamous cells of undetermined significance), 48.7% in LSIL group (low grade squamous intraepithelial lesions), and 71.9% in HSIL group (high grade squamous intraepithelial lesions) (p-value < 0.001). High-risk (HR) HPV prevalence appeared to be different from other areas of the world; we have detected a high prevalence rate of HPV-16, 31, and 58 and a low prevalence rate of HPV-18 and 11. The prevalence of both HR and low risk (LR) genotype groups was clearly related to age (p-value < 0.001), since the prevalence of LR group had a nadir between 41 and 50 y of age and 2 peaks at 15-20 y and at over 60 y, while the curve of prevalence of HR genotypes displayed an almost inverse trend.

  11. The importance of immediate verification of a cervical cytological abnormality with histology

    Directory of Open Access Journals (Sweden)

    C Kabaca

    2013-01-01

    Full Text Available Background: A serious proportion of the patients with invasive cervical cancer can be women who have had abnormal smear findings known for at least 6 months. Aims: The aims of the study were to evaluate the cervical cytohistopathologic correlation in the population studied, and to discuss the acceptability of immediate histological verification for minor Papanicolaou smear abnormalities. Materials and Methods: A total of 443 patients who were admitted with abnormal smear results and had undergone immediate colposcopy, cervical biopsy and endocervical curretage in the gynecologic oncology clinic between the years of 2003-2009 were enrolled into the present retrospective study. One-way analysis of variance and independent t-tests were used to study the results. Results: The distribution of abnormal smear results were documented as 46.27%, 29.57%, 13.76%, 7.67%, 1.58%, 0.67%, and 0.45% for atypical squamous cells of undetermined significance (ASC-US, low-grade squamous intraepithelial lesion (LSIL, high-grade squamous intraepithelial lesion (HSIL, atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (ASC-H, squamous cell carcinoma (SCC, atypical glandular cell (AGC, and adenocarcinoma, respectively. The percentages of cervical intraepithelial neoplasia grade 2-3 (CIN 2-3 and greater lesions were 70.49%, 35.29%, 15.26%, and 9.75% for HSIL, ASC-H, LSIL, and ASC-US, respectively. Moreover, 38.36% of all the CIN 2-3 or cancer (n = 104 cases originated from those with low grade referral diagnosis (ASC-US and LSIL. Conclusions: The majority of cases in the study were predominantly ASC-US and LSIL and approximately 40% of all the high grade lesions came from those with low grade referral diagnosis. This shows poor cytohistopathological correlation and calls the triage of minor cytological abnormalities into question.

  12. Histological Correlation of Glandular Abnormalities in Cervical Liquid-Based Cytology

    OpenAIRE

    Yosuke Kawakami; Tamaki Toda; Toshinao Nishimura; Junichi Sakane; Kazuya Kuraoka; Kazuhiro Takehara; Tomoya Mizunoe; Kiyomi Taniyama

    2011-01-01

    Conventional Papanicolaou smear method is still commonly used for cervical cancer screening in Japan, despite the liquid-based cytology (LBC) that has become a global tendency in the world recently. One of the obstacles in the way of popularization of this method seems to be the confusion as to diagnosis upon cervical glandular lesions. We performed comparison study between LBC and conventional Papanicolaou smear about cytological diagnosis using split-sample method in 4522 patients. In 13 ca...

  13. Cervical cytology in serous and endometrioid endometrial cancer.

    Science.gov (United States)

    Roelofsen, Thijs; Geels, Yvette P; Pijnenborg, Johanna M A; van Ham, Maaike A P C; Zomer, Saskia F; van Tilburg, Johanna M Wiersma; Snijders, Marc P M L; Siebers, Albert G; Bulten, Johan; Massuger, Leon F A G

    2013-07-01

    The aim of this study was to determine the frequency of abnormal cervical cytology in preoperative cervical cytology of patients diagnosed with uterine papillary serous carcinoma (UPSC) and endometrioid endometrial carcinoma (EEC). In addition, associations between abnormal cervical cytology and clinicopathologic factors were evaluated. In this multicentre study, EEC patients diagnosed at two hospitals from 1999 to 2009 and UPSC patients diagnosed at five hospitals from 1992 to 2009, were included. Revision of the histologic slides was performed systematically and independently by 3 gynecopathologists. Cervical cytology within six months before histopathologic diagnosis of endometrial carcinoma was available for 267 EEC and 80 UPSC patients. Cervical cytology with atypical, malignant, or normal endometrial cells in postmenopausal women was considered as abnormal cytology, specific for endometrial pathology. Abnormal cervical cytology was found in 87.5% of UPSC patients, compared with 37.8% in EEC patients. In UPSC, abnormal cytology was associated with extrauterine spread of disease (P=0.043). In EEC, abnormal cytology was associated with cervical involvement (P=0.034). In both EEC and UPSC patients, abnormal cervical cytology was not associated with survival. In conclusion, abnormal cervical cytology was more frequently found in UPSC patients. It was associated with extrauterine disease in UPSC patients, and with cervical involvement in EEC patients. More prospective research should be performed to assess the true clinical value of preoperative cervical cytology in endometrial cancer patients. PMID:23722512

  14. Polymerase chain reaction and conventional DNA tests in detection of HPV DNA in cytologically normal and abnormal cervical scrapes

    DEFF Research Database (Denmark)

    Kalia, A.; Jalava, T.; Nieminen, P.;

    1992-01-01

    Med.mikrobiologi, polymerase chain reaction, DNA tests, human papillomavirus (HPV), cervical smear, hybridisation, cytologi, affiProbe HPV test, ViraType test......Med.mikrobiologi, polymerase chain reaction, DNA tests, human papillomavirus (HPV), cervical smear, hybridisation, cytologi, affiProbe HPV test, ViraType test...

  15. Genotype Distribution of Human Papillomavirus among Women with Cervical Cytological Abnormalities or Invasive Squamous Cell Carcinoma in a High-Incidence Area of Esophageal Carcinoma in China.

    Science.gov (United States)

    Wang, Yuanyuan; Wang, Shaohong; Shen, Jinhui; Peng, Yanyan; Chen, Lechuan; Mai, Ruiqin; Zhang, Guohong

    2016-01-01

    Data of HPV genotype including 16 high-risk HPV (HR-HPV) and 4 low-risk HPV from 38,397 women with normal cytology, 1341 women with cervical cytology abnormalities, and 223 women with ISCC were retrospectively evaluated by a hospital-based study. The prevalence of high-risk HPV (HR-HPV) was 6.51%, 41.83%, and 96.86% in women with normal cytology, cervical cytology abnormalities, and ISCC, respectively. The three most common HPV types were HPV-52 (1.76%), HPV-16 (1.28%), and HPV-58 (0.97%) in women with normal cytology, whereas the most prevalent HPV type was HPV-16 (16.85%), followed by HPV-52 (9.55%) and HPV-58 (7.83%) in women with cervical cytology abnormalities. Specifically, HPV-16 had the highest frequency in ASC-H (24.16%, 36/149) and HSIL (35.71%, 110/308), while HPV-52 was the most common type in ASC-US (8.28%, 53/640) and LSIL (16.80%, 41/244). HPV-16 (75.78%), HPV18 (10.31%), and HPV58 (9.87%) were the most common types in women with ISCC. These data might contribute to increasing the knowledge of HPV epidemiology and providing the guide for vaccine selection for women in Shantou. PMID:27610364

  16. Predictors of persistent cytologic abnormalities after treatment of cervical intraepithelial neoplasia in Soweto, South Africa: a cohort study in a HIV high prevalence population

    Directory of Open Access Journals (Sweden)

    McIntyre James A

    2008-07-01

    Full Text Available Abstract Background In the presence of both HIV infection and cervical intraepithelial neoplasia (CIN, the risk of cancer development despite treatment may be greater. We investigated clinical predictors of persistent cytological abnormalities in women who had had a large loop excision of the transformation zone (LLETZ. Methods Women with high grade squamous intraepithelial lesions or worse (HSIL, less severe abnormalities which persisted and any abnormality in women who are HIV-infected, were referred to the colposcopy clinic. HIV infection was ascertained by self-report. A LLETZ was performed on all patients with HSIL or higher on Papanicolaou (Pap smear or colposcopy, LSIL or higher in patients who are HIV-infected, where the colposcopy is inadequate, and when there was a discrepancy between colposcopy and cytology by one or more grades. Women with abnormal follow-up smears were compared to those with normal smears. We examined the association between abnormal follow-up smears and demographic and clinical predictors using logistic regression Results The median time between LLETZ and first follow-up Pap smear was rather short at 122 days. Persistent cytological abnormalities occurred in 49% of our patients after LLETZ. Predictors of persistence included the presence of disease at both margins and HIV infection. Among the latter, disease at the excision margins and CD4+ cell count were important predictors. In these women, disease at the endocervical margin, both margins, and disease only at the ectocervical margin were associated with increased odds of persistent abnormalities on follow-up cervical smear. Conclusion We showed extremely high risk of cytological abnormality at follow-up after treatment more so in patients with incomplete excision and in the presence of immunocompromise. It remains uncertain whether recurrent CIN is a surrogate marker for invasive cervical cancer.

  17. Human papillomavirus infection and anxiety: analyses in women with low-grade cervical cytological abnormalities unaware of their infection status.

    Directory of Open Access Journals (Sweden)

    Candice Y Johnson

    Full Text Available BACKGROUND: Women testing positive for human papillomavirus (HPV infection experience increased levels of anxiety that have been attributed to fears of stigmatization and developing cervical cancer. The objective of this study was to investigate the association between HPV infection and anxiety in women who were unaware they had been tested specifically for HPV, to determine if any anxiety experienced by HPV-positive women could be due to causes other than learning of test results. METHODS: This study was nested within a randomised controlled trial of management of women with abnormal cervical cytology conducted in the United Kingdom with recruitment between 1999 and 2002. At baseline, prior to having a sample taken for HPV testing, the results of which were not disclosed, women were assessed for anxiety using the Hospital Anxiety and Depression Scale and asked about fears of developing cervical cancer ("cancer worries"; this assessment was repeated at 12, 18, 24, and 30 months of follow-up. Logistic regression and generalized estimating equations were used for the cross-sectional (baseline and longitudinal analyses, respectively. RESULTS: Among the 2842 participants, there was no association between HPV status and anxiety among white women. Among non-white women, however, anxiety was less common among HPV-positive than HPV-negative women (adjusted odds ratio 0.41, 95% confidence interval 0.22 to 0.77. Among non-smokers, cancer worry was more common in HPV-positive than HPV-negative women; the opposite association was observed among ex-smokers. CONCLUSIONS: Associations between HPV status and anxiety may be explained by factors other than learning of test results and may vary by ethnicity and lifestyle factors.

  18. Management of Cervical Cytology with HPV Test

    Centers for Disease Control (CDC) Podcasts

    2009-10-15

    Dr. Stewart Massad, a professor in the Division of Gynecologic Oncology at Washington University in Saint Louis and a board member of the American Society for Colposcopy and Cervical Cancer Prevention (ASCCP), talks about ASCCP's 2006 Consensus Guidelines on the management of abnormal cervical cytology and histology.  Created: 10/15/2009 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP), Division of Cancer Prevention and Control (DCPC).   Date Released: 6/9/2010.

  19. CIGARETTE-SMOKING AND HUMAN PAPILLOMAVIRUS IN PATIENTS WITH REPORTED CERVICAL CYTOLOGICAL ABNORMALITY

    NARCIS (Netherlands)

    BURGER, MPM; HOLLEMA, H; GOUW, ASH; PIETERS, WJLM; QUINT, WGV

    1993-01-01

    Objective-To assess the relation between two risk factors for cervical neoplasia: smoking and infection with oncogenic human papillomavirus. It has been suggested that smoking causes a local immunological defect, which could facilitate the infection and persistence of human papillomavirus. Design-Cr

  20. Prevalence and cumulative incidence of abnormal cervical cytology among HIV-infected Thai women: a 5.5-year retrospective cohort study

    Directory of Open Access Journals (Sweden)

    Thamkhantho Manopchai

    2011-01-01

    Full Text Available Abstract Background Cervical cancer is one of the most common AIDS-related malignancies in Thailand. To prevent cervical cancer, The US Public Health Service and The Infectious Disease Society of America have recommended that all HIV-infected women should obtain 2 Pap smears 6 months apart after the initial HIV diagnosis and, if results of both are normal, should undergo annual cytological screening. However, there has been no evidence in supporting whether this guideline is appropriate in all settings - especially in areas where HIV-infected women are living in resource-constrained condition. Methods To determine the appropriate interval of Pap smear screenings for HIV-infected Thai women and risk factors for subsequent abnormal cervical cytology, we assessed the prevalence, cumulative incidence and associated factors of cervical cell abnormalities (atypical squamous cell of undetermined significance or higher grades, ASCUS+ among this group of patients. Results The prevalence of ASCUS+ was 15.4% at the first visit, and the cumulative incidence of ASCUS+ gradually increased to 37% in the first 3.5 years of follow-up appointments (first 7 times, and tended to plateau in the last 2 years. For multivariate correlation analysis, women with a CD4 count P = 0.043. There were no associations of subsequent ASCUS+ with age, pregnancy, contraceptive method, highly active anti-retroviral treatment, assumed duration of infection, or the CD4 count nadir level. Conclusion There are high prevalence and cumulative incidence of ASCUS+ in HIV-infected Thai women. With a high lost-to-follow-up rate, an appropriate interval of Pap smear screening cannot be concluded from the present study. Nevertheless, the HIV-infected Thai women may require more than two normal semi-annual Pap smears before shifting to routinely annual cytologic screening.

  1. Study of the abnormal cervical-vaginal cytology of sexually active young women living within the Waterloo region.

    Science.gov (United States)

    Redmond, M; Schumacher, B

    1990-01-01

    618 women aged 13-36 attending a family planning clinic took part in a sexual health survey during 1985 and 1986. They were followed up for one year in order to ascertain whether an increase in atypical and dysplastic pap smears was linked to a certain lifestyle. The routine examination included a pap smear, a cervical swab for gonorrhea and other vaginal flora. Cultures for herpes and chlamydia were done. Benign atypia infection was treated followed by repeat pap smears 6-8 weeks and 2-3 months later. 7% of the women were virgins, 72% had smoked, and 62% presently smoked (an average of 9 cigarettes daily). Average age at first intercourse, was 16, and partners ranged from 1 to 35. Condom use was most prevalent: 15% used it "always", 20% "often", and 36% "occasionally". The oral contraceptive use pattern was: 60% "always" used it and 10% "often" relied on it. 1/4th used the rhythm method. 90% had never been pregnant, 9% had, and 1% were unsure. of 49 pregnancies 41 ended in abortion. After examining 581, 4 cases condylomata and 1 case of genital herpes were found. 60% of the women had normal cervical cytology, 32% showed atypia, and 8% displayed dysplasia. 22% of the atypias were linked to inflammation and 6% were related to yeast. 9% of repeat atypia or dysplasia was less severe by biopsy, while 54% turned out to be more severe than the pap smear results, although they tend to indicate more severe changes. 8% showed less severe cytology, and 59% more severe cytological changes. 78% of the group with mild dysplasia had smoked, as opposed to 22% that had "never smokes". Those with mild dysplasia were 4 times more likely to smoke at the time of their initial visit (79%) than nonsmokers (21%). 54% of those with normal pap smear reported "often" or "always" using condoms, whereas only 10% of the group with dysplasia reported similar use pattern. These findings uphold the practice of following the repeated atypical, or initial dysplastic pap smear results with

  2. A Virtual Microscopy System to Scan, Evaluate and Archive Biomarker Enhanced Cervical Cytology Slides

    Directory of Open Access Journals (Sweden)

    Niels Grabe

    2010-01-01

    Full Text Available Background: Although cytological screening for cervical precancers has led to a reduction of cervical cancer incidence worldwide it is a subjective and variable method with low single-test sensitivity. New biomarkers like p16 that specifically highlight abnormal cervical cells can improve cytology performance. Virtual microscopy offers an ideal platform for assisted evaluation and archiving of biomarker-stained slides.

  3. The probability for a Pap test to be abnormal is directly proportional to HPV viral load: results from a Swiss study comparing HPV testing and liquid-based cytology to detect cervical cancer precursors in 13,842 women.

    Science.gov (United States)

    Bigras, G; de Marval, F

    2005-09-01

    In a study involving 13,842 women and 113 gynaecologists, liquid-based cytology and HPV testing for detecting cervical cancer were compared. A total of 1334 women were found to be positive for one or both tests and were invited for colposcopy with biopsy. A total of 1031 satisfactory biopsies on 1031 women were thereafter collected using a systematic biopsy protocol, which was random in the colposcopically normal-appearing cervix or directed in the abnormal one. In all, 502 women with negative tests were also biopsied. A total of 82 histologic high-grade squamous intraepithelial lesion (HSIL) were reported in biopsies, all from the group with one or both tests positive. Sensitivity and specificity to detect histologic HSIL were 59 and 97% for cytology, and 97 and 92% for HPV. In total, 14% of reviewed negative cytological preparations associated with histologic HSIL contained no morphologically abnormal cells despite a positive HPV test. This suggested a theoretical limit for cytology sensitivity. HPV viral load analysis of the 1143 HPV-positive samples showed a direct relationship between abnormal Pap test frequency and HPV viral load. Thus, not only does the HPV testing have a greater sensitivity than cytology but the probability of the latter being positive can also be defined as a function of the associated HPV viral load. PMID:16136031

  4. The probability for a Pap test to be abnormal is directly proportional to HPV viral load: results from a Swiss study comparing HPV testing and liquid-based cytology to detect cervical cancer precursors in 13 842 women

    Science.gov (United States)

    Bigras, G; de Marval, F

    2005-01-01

    In a study involving 13 842 women and 113 gynaecologists, liquid-based cytology and HPV testing for detecting cervical cancer were compared. A total of 1334 women were found to be positive for one or both tests and were invited for colposcopy with biopsy. A total of 1031 satisfactory biopsies on 1031 women were thereafter collected using a systematic biopsy protocol, which was random in the colposcopically normal-appearing cervix or directed in the abnormal one. In all, 502 women with negative tests were also biopsied. A total of 82 histologic high-grade squamous intraepithelial lesion (HSIL) were reported in biopsies, all from the group with one or both tests positive. Sensitivity and specificity to detect histologic HSIL were 59 and 97% for cytology, and 97 and 92% for HPV. In total, 14% of reviewed negative cytological preparations associated with histologic HSIL contained no morphologically abnormal cells despite a positive HPV test. This suggested a theoretical limit for cytology sensitivity. HPV viral load analysis of the 1143 HPV-positive samples showed a direct relationship between abnormal Pap test frequency and HPV viral load. Thus, not only does the HPV testing have a greater sensitivity than cytology but the probability of the latter being positive can also be defined as a function of the associated HPV viral load. PMID:16136031

  5. Cervical cytology screening convenient for the workforce.

    Science.gov (United States)

    Andren, S M

    1991-01-01

    The study describes the demographic details and smear results of women attending a Well Woman Clinic in an Occupational Health setting over a two year period. The clinic is located close to the place of work, run during working hours, and operated by a doctor and nurse. Charging fees acceptable to women attending or their employers, the service has made a modest profit and offers scope for increasing the income against fixed costs. The age range attending is older than commonly found at screening clinics. The abnormal smear detection rate, 115 per 1000 is high while the proportion of inadequate results at 50 per 1000 compares favourably with other clinics. In an area with 13 per cent Asian population, only 5 per cent of attenders were Asian but of these one fifth had abnormal smears. A cervical cytology screening facility timed and located for the convenience of the working woman is found to attract a group of women in the older age range who have a high abnormal smear rate, and those who fail to attend elsewhere. PMID:1779673

  6. Cytological diagnosis in cervical cancer

    OpenAIRE

    Mariana Bratu; Florentina Pricop; Ovidiu Toma; Dragos Crauciuc; Eduard Crauciuc

    2010-01-01

    Aim. The cytological test has multiple valences, allowing the early discovery and location of feminine genital cancer. Material and methods. In the period of time between 2001 and 2009, the study made within the Obstetrics and Gynecology Department of „Sf. Apostol Andrei” Emergency Hospital in Galaţi, revealed that from 415 cases with a changed PAP smear, the cytological diagnosis showed cancerous and pre-cancerous lesions in 53 patients (12.8%). We harvested cytological smears fo...

  7. Rastreamento anterior para câncer de colo uterino em mulheres com alterações citológicas ou histológicas Previous screening for cervical cancer among women with cytological and histological abnormalities

    Directory of Open Access Journals (Sweden)

    C Rama

    2008-06-01

    do último exame citológico anterior.OBJECTIVE: To examine previous Pap smear history in women screened for cervical cancer with cytological or histological abnormalities. METHODS: Cross-sectional study in a sample of 5,485 women (15-65 years old who self-referred to cervical cancer screening in Sao Paulo and Campinas, Southeastern Brazil, between February 2002 and March 2003. A behavioral questionnaire was applied and cervical specimens were obtained for testing by Pap smears or liquid-based cytology. Women who had abnormal cytology were referred for colposcopic examination and, if abnormal, for cervical punch biopsy. To explore factors associated to cervical abnormalities Pearson's chi-was conduted square test at a 5% significance level. RESULTS: Cytological abnormalities were found in 354 women (6.4% and included 41 high-grade squamous intra-epithelial lesions and 3 carcinomas; 92.7% were normal results. Colposcopy was performed in 289 women, and 145 (50.2% showed abnormal results. Punch biopsies showed 14 cervical intraepithelial neoplasias grade 3 and 4 carcinomas. Previous Pap smears were reported in all women who had cytology suspected of carcinoma, 97.6% of those with high-grade squamous intra-epithelial lesions, all women with histological diagnosis of carcinoma and 92.9% of those who had cervical intraepithelial neoplasias grade 3 histologically. Previous Pap smear in the last tree years was reported by 86.5% and 92.8% of women with abnormal cytology and biopsy, respectively. CONCLUSIONS: There was no statistically significant difference regarding the number of Pap tests and time since their last test between women with histologically diagnosed carcinoma and cervical intraepithelial neoplasia grade 3 compared with those with normal cytology.

  8. CERVICAL CYTOLOGICAL SCREENING AND MANAGEMENT IN PREGNANT AND POSTPARTUM WOMEN

    Institute of Scientific and Technical Information of China (English)

    Gui-fang He; Mei-lu Bian; Ying Wang; Xiao-yan Liu

    2005-01-01

    Objective To examine and follow up cervical cytology of pregnant and postpartum women and study their cytopathologic characteristics, so as to determine screening and managing programs for abnormal cervical cytology.Methods Totally 5296 patients in pregnancy and postpartum, in which 3729 by computer-assisted cytology test and 1567 by liquid-based monolayers cytology test, were examined and diagnosed by the Bethesda System made in 2001. Those proven epithelial abnormalities patients were followed up until the lesions regressed to normal. The remaining patients,who exhibited persistent abnormalities or progression, were given further examinations.Results The positive rate of cervical cytological test was 8.72% (462/5296), including squamous carcinoma (SCA) 1case (0.02%), high grade squamous intraepithelial lesion (HSIL) 34 cases (0.64%), low grade squamous intraepithelial lesion (LSIL) 145 cases (2.74%), atypical glandular cells (AGC) 5 cases (0.09%), atypical squamous cells cannot exclude HSIL (ASC-H) 14 cases (0.26%), atypical squamous cells of undetermined significance (ASC-US) 263 cases (4.97%). The 419 proven cytological abnormality cases were followed up successfully. The total transnegative rate in three months was 73.74% (309/419), in which 303 cases (72.32%) persisted normal status for more than six months after regression. And the transnegative rate of ASC-US, ASC-H, AGC, LSIL, and HSIL were 79.56%, 64.29%, 100%,72.14% and 44.12%, respectively. Forty-six cases received biopsy directed by colposcopy. The distribution of coincidence of cytopathologic and histopathologic diagnosis was: SCA 1 case (100%), HSIL 10 cases (76.92%), LSIL 13 cases (65%), ASC-H 2 cases (50%), ASC-US 3 cases (37.50%), total 29 cases (63.04%).Conclusions We should cast more attention to screening cervix lesions in pregnant and postpartum women. Their cytopathologic characteristics are liable to make the clinician give a false positive diagnosis. So we propose to follow up them closely

  9. Letter: Contraceptive choice and cervical cytology.

    Science.gov (United States)

    Leppaluoto, P

    1974-02-15

    In the evaluation of biological potentials of contraceptives in the genesis of cervical cancer, cytology seems to have been given due attention while information on the clinical condition and specific treatment of the target organ, the cervix, is repeatedly neglected. "Cancer does not develop in a healthy cervix" is an old dogma derived from impressions gained by clinical examination of the organ. This concept is supported by epidemiologic studies indicating that cancer is extremely rare in cervices restored to a normal condition by cauterization. It may be postulated that this type of treatment destroys the permissive target cells of the cervix, thus indirectly rendering possible coitus-associated and contraceptive-associated(?) mutagen(s) invalid. Also, in comparative studies, an evaluation should be made of the significance of a higher incidence of cervical ectopy reported in women receiving oral hormonal contraceptives. The importance of ectopy in the genesis of cervical malignancy has been derived from the presumption that permissive cervical cells are thus created and exposed to vaginal contents which may harbor the mutagens(s). Therefore, it would seem not only desirable but imperative to include the neglected parameters of clinical condition and of specific treatment (both past and present) of the uterine cervix in the structure of studies like the recent one in this Journal on "Contraceptive choice and cervical cytology", by Drs. Shulman and Merritt. Still another parameter which might prove to be of significance is the predominant type of vaginal flora, which can be assessed readily in the routine examination of Papanicolaou smears. It may be that vaginal contents, which would seem to be somewhat different in wearers of the intrauterine contraceptive device as compared to "pill" users, might play a part in the advent of cervical malignancy as a medium of or for coitus-associated mutagens(s). PMID:4855868

  10. The cytological diagnosis and prognosis of malignization of cervical epithelium of uterus in pregnant women.

    OpenAIRE

    Sumenko V.V.

    2007-01-01

    The investigation is dedicated to the study of the pathological alterations of cervical epithelium of uterus, proliferative activity in the lesion focuses and the improvement of cytological diagnosis of the state of cervical epithelium of uterus. The correlation between the proliferative processes in abnormal epithelium of uterine cervix and some pathological factors of the pregnant women organism was studied. The criteria for prognosis of the cervical epithelial dysplasia in pregnant women w...

  11. Routine Treatment of Cervical Cytological Cell Changes

    Science.gov (United States)

    Huber, J.; Pötsch, B.; Gantschacher, M.; Templ, M.

    2016-01-01

    Introduction: Diagnosis and treatment of vaginal and cervical cytological cell changes are described in European and national guidelines. The aim of this data collection was to evaluate the remission rates of PAP III and PAP III D cytological findings in patients over a period of 3–4 months. Method: The current state of affairs in managing suspicious and cytological findings (PAP III, and III D) in gynecological practice was assessed in the context of a data collection survey. An evaluation over a period of 24 months was conducted on preventative measures, the occurrence and changes to normal/suspect/pathological findings and therapy management (for suspicious or pathological findings). Results: 307 female patients were included in the analysis. At the time of the survey 186 patients (60.6 %) had PAP III and 119 (38.8 %) had PAP III D findings. The spontaneous remission rate of untreated PAP III patients was 6 % and that of untreated PAP III D patients was 11 %. The remission rates of patients treated with a vaginal gel were 77 % for PAP III and 71 % for PAP III D. Conclusion: A new treatment option was used in gynecological practice on patients with PAP III and PAP III D findings between confirmation and the next follow-up with excellent success. PMID:27761030

  12. Outcomes in cervical screening using various cytology technologies

    DEFF Research Database (Denmark)

    Barken, Sidsel S; Rebolj, Matejka; Lynge, Elsebeth;

    2013-01-01

    Unlike for human papillomavirus screening, little is known about the possible age-dependent variation in the outcomes of cervical cytology screening. The aim of our study was to describe age-related outcomes of five cytological technologies in a population-based screening program targeting women......, the proportion almost doubled, relative proportion 1.96 (95% confidence interval: 1.84-2.08). An opposite development was seen in women aged 45-59 years, relative proportion 0.68 (95% confidence interval: 0.57-0.82). Technological upgrading of cytology strongly affected the outcome of cervical screening...... aged 23-59 years. All cervical cytology from women residing in Copenhagen has been analyzed in the laboratory of the Department of Pathology, Hvidovre University Hospital. We studied five technology phases: (1) conventional cytology with manual reading, (2) conventional cytology with 50% automatically...

  13. 妊娠期妇女子宫颈细胞学检查结果异常的相关因素分析%Factors associated with abnormal cervical cytology in pregnant women

    Institute of Scientific and Technical Information of China (English)

    范玲; 邹丽颖; 吴玉梅; 张为远

    2010-01-01

    Objective To investigate the risk factors associated with abnormal cervical cytology findings in pregnant women. Methods From Sep. 2007 to Sep. 2008, 12 112 pregnant women who underwent their antenatal examinations at 12-36 gostational weeks in Beijing Obsteties and Gynecology Hospital were enrolled in this study. They were all excluded from the following pathologic obstetrics factors including threatened abortion, premature rupture of membranes or placental previa Thinprep cytology test (TCT) were given at their first examination, meanwhile, a personal clinic file was established to record her occupation, education, address, family income, nationality, age of first intercourse, number of sex partners, contraception, marriage and pregnancy, current gynecologic diseases, family history of gynecologic tumors, history of gynecologic diseases and smoking and result of pelvic examination. Those risk factors leading to abnormal cervical cytology were analyzed. Results The complete clinical data were collected from 11 906 cases(98.30%, 11 906/12 112). It was found that 10 354 women were shown with normal TCT result, however, 1134 women (9.52%, 1134/11 906) with atypical squamaous cells of undetermined significance(ASCUS), 112 women (0.94%, 112/11 906) with atypical glandular cells of undetermined significance(AGUS), 229 women (1.92%, 229/11 906) with low grade squamaous intraepithelial(LSIL),74 women (0.62%, 74/11906) with high grade squamaous intraepithelial(HSIL). Multiple factorial non-conditioned logistic regression analysis showed that age of first sexual intercourse (OR_(ASCUS)=2.90, OR_(AGUS)=7. 32), number of sex partners (OR_(AGUS)=1.49, OR_(AGUS)=2. 02), number of abortion (OR_(ASCUS)=1.68,OR_(AGUS)=3.50) were correlated with ASCUS and AGUS. In LSIL group and HSIL group, age of first sexual intercourse (OR_(LSIL)=6. 34, OR_(HSIL)=9.26), number of sex partners (OR_(LSIL)=1.69, OR_(HSIL)=1.6.5),number of abortion (OR_(LSIL)=1.53, OR_(HSIL)=5. 33), smoking (OR

  14. Integrated cervical smear screening using liquid based cytology and bioimpedance analysis

    OpenAIRE

    Lopamudra Das; Tandra Sarkar; Maiti, Ashok K.; Sukla Naskar; Soumen Das; Jyotirmoy Chatterjee

    2014-01-01

    Objective: To minimize the false negativity in cervical cancer screening with Papanicolaou (Pap) test, there is a need to explore novel cytological technique and identification of unique and important cellular features from the perspectives of morphological as well as biophysical properties. Materials and Methods: The present study explores the feasibility of low-cost cervical monolayer techniques in extracting cyto-pathological features to classify normal and abnormal conditions. The cer...

  15. The status of cervical cytology in Swaziland, Southern Africa: A descriptive study

    Directory of Open Access Journals (Sweden)

    Okonda Sylvain

    2009-01-01

    Full Text Available Background: Cancer of the cervix is the most common cancer in women in Swaziland where most women never undergo cervical screening. The extremely high prevalence of HIV/AIDS in Swaziland complicates the management of preinvasive and invasive cervical cancer. The purpose of this study was to assess the current status of cervical cytology in Swaziland, its strengths and limitations. Methods: The study is a retrospective review of 12,188 conventional cervical smears received by the Central Public Health Laboratory in Swaziland from June 2004 to May 2006. Results: Review of results showed very high rates of cytologic abnormalities with 43.2% of smears screened reported as abnormal. The percentages of abnormalities were as follows: atypical squamous cells of undermined significance (ASC-US, 19.8%; atypical squamous cells, cannot exclude HSILs (ASC-H, 8.8%; low-grade squamous intraepithelial lesions (LSIL, 9.0%; high-grade squamous intraepithelial lesions (HSIL, 4.6%; squamous cell carcinomas, 0.5%; atypical endocervical cells, 0.6%; and atypical endometrial cells, 0.4%. Just over 5% of smears were inadequate. The highest rates of HSILs and invasive squamous carcinoma occurred in women aged 50-59 years. Conclusions: This study underscores the need to reduce the incidence of cervical cancer and its precursor lesions in Swaziland women. Based on studies of human papillomavirus (HPV types in other Southern African countries, current HPV vaccines would reduce the incidence and mortality from cervical cancer in the future, but cervical screening would still be required, both for women already infected with the HPV and for HPV subtypes not covered by current vaccines. The most cost-effective combination of screening modalities such as visual inspection, HPV DNA testing, and cytology should be investigated. Cervical cancer reduction needs to be managed within the greater framework of the HIV/AIDS epidemic.

  16. CpG methylation in human papillomavirus (HPV) type 31 long control region (LCR) in cervical infections associated with cytological abnormalities.

    Science.gov (United States)

    László, Brigitta; Ferenczi, Annamária; Madar, László; Gyöngyösi, Eszter; Szalmás, Anita; Szakács, Levente; Veress, György; Kónya, József

    2016-08-01

    The mechanisms that regulate papillomavirus gene expression include DNA methylation. The transcription of papillomavirus oncogenes E6 and E7 is controlled by certain regulatory elements in the LCR, which include binding sites for the E2 protein, a viral regulator of oncogene expression. In HPV-31-infected exfoliated cervical cells, the CpG methylation of the entire LCR was determined by next-generation sequencing after bisulfite modification. Six of the 22 cases had methylated CpG sites in the HPV-31 LCR, including position 7479 and/or 7485, at the promoter distal E2 binding site, thus suggesting a potential regulatory mechanism for papillomavirus transcription. PMID:27098644

  17. Two cytological methods for screening for cervical cancer

    DEFF Research Database (Denmark)

    Kirschner, B.; Simonsen, K.; Junge, J.

    2008-01-01

    INTRODUCTION: Denmark has had an organized screening programme for cervical cancer since the 1960s. In spite of this, almost 150 Danish women die from the disease each year. There are currently two different methods for preparation of cervical samples: conventional Papanicolaou smear and liquid......-based cytology. MATERIALS AND METHODS: In 2002, the Department of Pathology, Hvidovre Hospital changed over from the conventional Papanicolaou smear screening method to SurePath liquid-based cytology. This article is based on a retrospective comparison on data from the population screening programme for cervical...... cancer in the Municipality of Copenhagen. RESULTS: The number of tests with the diagnosis of "normal cells" decreased 1% after the conversion to liquid-based cytology, whilst the number of tests with "atypical cells" and "cells suspicious for malignancy" increased by 64.3% and 41.2% respectively...

  18. CD-ROM transnational training program in cervical cytology (CYTOTRAIN).

    Science.gov (United States)

    Marsan, C; Coleman, D V; Branca, M; Cochand-Priollet, B; Molinié, V

    2001-01-01

    The Transnational Training Programme in Cervical Cytology (CYTOTRAIN) is a 3-yr project funded by the European Commission to harmonize training and quality standards in cervical screening across the European Union. The aim of the program is to develop new approaches in initial and continuing vocational training, particularly in the area of life-long learning with the aim of meeting national, regional, and local needs. We present a new approach to training in cervical cytology, using an interactive program of cytological images. The method used to prepare the program and the problems encountered are described. The authors have the feeling that giving details of the organizational and management structure adopted for the project implementation might help other pathologists realize more or less similar CD-ROM training programs in their own field of activity. PMID:11135473

  19. Association of Trichomonas vaginalis and cytological abnormalities of the cervix in low risk women.

    Directory of Open Access Journals (Sweden)

    Gilbert G G Donders

    Full Text Available OBJECTIVE: Is Trichomonas vaginalis (TV an inducing factor for the development of (pre-cancerous lesions of the cervix? DESIGN: Cross sectional study. SETTING: Screening healthy Belgian women with low infection risk. SAMPLE: 63,251 consecutive liquid based cervical samples. METHODS: Real time quantitative PCR for presence of TV, 18 HPV types and Pap smear analysis of cytologic abnormalities. MAIN OUTCOME MEASURES: Association of TV and HPV with cervix dysplasia. RESULTS: The overall prevalence of TV DNA was 0.37%, of low risk HPV 2%, of high risk HPV 13.2%, and 8.8 % had cytological abnormalities. Both LR-HPV and HR-HPV were significantly associated with all cytological abnormalities. Presence of TV was associated with LR- and HR-HPV, ASC-US and HSIL, but not with other abnormalities. All women with TV and HSIL also had HR-HPV, while the latter was present in only 59% of women with TV and ASC-US. Amongst HPV negative women, TV was found in 1.3% of women with ASC-US, but only in 0.03% of women with normal cytology (OR 4.2, CL95% 2.1-8.6. In HR-HPV positive women, presence of TV increased the likelihood of cytological abnormalities somewhat (P=0.05, mainly due to an increase in ASC-US and LSIL, but not HSIL. CONCLUSIONS: We conclude that TV infection is associated with both LR and HR-HPV infection of the cervix, as well as with ASC-US and HSIL. TV is a concomitant STI, but is not thought to be a co-factor in the causation of HSIL and cervical cancer. However, TV may cause false positive diagnoses of ASC-US.

  20. Automatic recognition of abnormal cells in cytological tests using multispectral imaging

    Science.gov (United States)

    Gertych, A.; Galliano, G.; Bose, S.; Farkas, D. L.

    2010-03-01

    Cervical cancer is the leading cause of gynecologic disease-related death worldwide, but is almost completely preventable with regular screening, for which cytological testing is a method of choice. Although such testing has radically lowered the death rate from cervical cancer, it is plagued by low sensitivity and inter-observer variability. Moreover, its effectiveness is still restricted because the recognition of shape and morphology of nuclei is compromised by overlapping and clumped cells. Multispectral imaging can aid enhanced morphological characterization of cytological specimens. Features including spectral intensity and texture, reflecting relevant morphological differences between normal and abnormal cells, can be derived from cytopathology images and utilized in a detection/classification scheme. Our automated processing of multispectral image cubes yields nuclear objects which are subjected to classification facilitated by a library of spectral signatures obtained from normal and abnormal cells, as marked by experts. Clumps are processed separately with reduced set of signatures. Implementation of this method yields high rate of successful detection and classification of nuclei into predefined malignant and premalignant types and correlates well with those obtained by an expert. Our multispectral approach may have an impact on the diagnostic workflow of cytological tests. Abnormal cells can be automatically highlighted and quantified, thus objectivity and performance of the reading can be improved in a way which is currently unavailable in clinical setting.

  1. The cytological diagnosis and prognosis of malignization of cervical epithelium of uterus in pregnant women.

    Directory of Open Access Journals (Sweden)

    Sumenko V.V.

    2007-01-01

    Full Text Available The investigation is dedicated to the study of the pathological alterations of cervical epithelium of uterus, proliferative activity in the lesion focuses and the improvement of cytological diagnosis of the state of cervical epithelium of uterus. The correlation between the proliferative processes in abnormal epithelium of uterine cervix and some pathological factors of the pregnant women organism was studied. The criteria for prognosis of the cervical epithelial dysplasia in pregnant women were elaborated on this basis of our data. The pathological processes in cervical epithelium were revealed in 309 pregnant women in the first trimester (mean age of women was 26,17±0,83 years. The hormonal state of pregnant women was studied on the basis of colpocytological picture and the colpocytological types which indicate the unfavorable prognosis with a certain degree of validity were determined. The mathematic model for all cytological groups of pregnant women for prognosis of inflammatory processes, epithelial dysplasia and preinvasive cervical cancer was created. The simple and comprehensible prognostic scheme for diagnosis of epithelial dysplasia and preinvasive cervical cancer was developed.

  2. Liquid-based cytology versus conventional cytology for evaluation of cervical Pap smears: Experience from the first 1000 split samples

    Directory of Open Access Journals (Sweden)

    Vikrant Bhar Singh

    2015-01-01

    Full Text Available Context and Aim: Screening programs using conventional cytology conventional Pap smear (CPS have successfully reduced cervical cancer, but newer tests like liquid-based cytology (LBC and human papillomavirus testing might enhance screening. The main aim of the present study was to assess the diagnostic accuracy of LBC versus CPS using "split samples." Materials and Methods: This was a prospective study comprising of 1000 consecutive cervical "split samples" over a period of 1 year. Split sample was obtained using cervex-brush. CPS was prepared from the brush and the brush head was suspended in the LBC vial and processed by SurePath™ LBC. Results: There were 4.3% unsatisfactory (U/S cases in CPS and 1.7% in LBC; the main cause is insufficient cells, and excess of blood in CPS. About 25/100 (2.5% split samples had epithelial abnormalities both in CPS and LBC (1.2%-atypical squamous cells of undetermined significance; 0.4%-low grade squamous intraepithelial lesion; 0.2%-high grade squamous intraepithelial lesion; 0.5%-squamous cell carcinoma; 0.1%-atypical glandular cells favouring neoplasia; 0.2%-adenocarcinoma. Inflammatory organisms were almost equally identified in both techniques but were better seen in LBC samples. Conclusions: LBC technique leads to significant reduction of U/S rate. LBC samples offered better clarity, uniform spread of smears, less time for screening and better handling of hemorrhagic and inflammatory samples. LBC had equivalent sensitivity and specificity to CPS.

  3. APPLICATION OF THE 2014 BETHESDA SYSTEM FOR REPORTING OF CERVICAL/VAGINAL CYTOLOGICAL LESIONS

    Directory of Open Access Journals (Sweden)

    Pratima

    2015-12-01

    Full Text Available Pap smear (Conventional smear is the most widely used cervical cancer screening test in the world. Negative intraepithelial lesion for malignancy, squamous intraepithelial lesion and cervical cancer remain important health problems for women worldwide. In developing countries like India, there is a great need for mass screening program for cervical lesion. AIMS AND OBJECTIVES To assess the clinician utility of The 2014 Bethesda System Reporting of cervical and vaginal epithelial abnormalities. MATERIAL AND METHODS All the cervical/vaginal cytology smears reported were retrieved and reviewed in the duration from 2007, 2008, 2009 and 2014. RESULTS A total number of 1700 cases of Pap smears were retrieved during period of study, out of which 90.0% were satisfactory for evaluation; 35.5% were within normal limit and 55.6% were NILM. A few (8.14% of the cases were reported as squamous intraepithelial lesion; 0.5% as atypical squamous cells and 0.19% were reported as atypical glandular cells. CONCLUSION Pap smear is a proven tested tool for making an early diagnosis and treating cervical cancer in early stage. Thus, Pap smear is simple, less expensive diagnostics tool suitable for implementation in India.

  4. ABC3 Part I: a review of the guidelines for terminology, classification and management of cervical cytology in England.

    Science.gov (United States)

    Smith, J H F

    2012-12-01

    The provision of guidance on cytology reporting and evaluation, first outlined in 1995 with the publication of Achievable Standards, Benchmarks for Reporting, and Criteria for Evaluating Cervical Cytopathology (ABC), and subsequently revised and expanded in a second edition in 2000, has been pivotal to the success of the National Health Service Cervical Screening Programme (NHSCSP), ensuring that standards are upheld, and that rigorous evaluation and quality assurance take place. In the last decade, major changes to the NHSCSP, notably the adoption of revised age ranges and screening intervals for all women in England, implementation of liquid-based cytology and, most recently, the decision to introduce high-risk human papillomavirus (HR-HPV) testing for triage of low-grade and borderline (equivalent to 'atypical') cytological abnormalities and test of cure after treatment of cervical intraepithelial neoplasia (CIN) determined that an updated version of ABC was required. The third edition of ABC recommends adoption, with minor modification, of the revised British Society for Clinical Cytology terminology and provides guidance on the management of abnormal cytology results linked to this terminology taking account of HR-HPV testing. To accommodate these changes, expanded result codes, which are electronic codes used to transfer management information to central computers for follow-up, call and recall of individual women, have been developed. Further guidance on specimen adequacy is also provided. Revised performance indicators are described and explained in a separate article by R. Blanks in this issue of Cytopathology. All the changes in ABC3 are designed to support the mission statement of the NHSCSP that 'the objective of cervical screening is to reduce cervical cancer incidence and mortality by screening with a high sensitivity for the detection of CIN2 or worse, whilst maintaining a high specificity'. PMID:23173804

  5. COMPARATIVE STUDY OF CYTOLOGIC AND COLPOSCOPIC FINDINGS IN PRECLINICAL CERVICAL CANCER

    Directory of Open Access Journals (Sweden)

    Penagaluru

    2015-11-01

    Full Text Available BACKGROUND The cytologic diagnosis of cervical smears has become a very important screening test for the detection of pre-invasive and invasive cervical epithelial abnormalities. MATERIALS AND METHODS It is a prospective study conducted for a period of 1 year in 100 women who fulfilled the inclusion criteria. Colposcopy, PAP smear and biopsy were done. RESULTS Majority 70.5% i.e., (12/17 of CIN occurred in the age group of 30-49 years. Among the 9 women who took OCP, 12% (2/17 had CIN. Incidence of CIN in the permanently sterilized group was 59% (10/17 and among IUCD user was 5.9% (1/17. Among women who were diagnosed to have CIN, 70.5% (12/17 complained of excessive vaginal discharge 11.7% (2/17 of women had post-coital bleeding. PAP smear had a sensitivity of 29% and a specificity of 88% which was attributed to the high number of false, negative smears. Colposcopy showed a sensitivity of 82% and a specificity of 81%. Sensitivity was more than pap smear but specificity was less than pap smear. Accuracy of Colposcopy was found to be 82% which was comparatively more accurate than pap smear (78%. CONCLUSIONS COLPOSCOPY offers an excellent tool in evaluating cervical lesions. It is an easy and perspective method and its importance lies in teaching, diagnosis and management of cervical lesions, both neoplastic and non-neoplastic.

  6. Accurate assessment of cell density in low cellular liquid-based cervical cytology

    NARCIS (Netherlands)

    Siebers, A.G.; Laak, J.A.W.M. van der; Huberts-Manders, R.; Vedder, J.E.M.; Bulten, J.

    2013-01-01

    A. G. Siebers, J. A. W. M. van der Laak, R. Huberts-Manders, J. E. M. Vedder and J. Bulten Accurate assessment of cell density in low cellular liquid-based cervical cytology Objective: Scant cellularity is the most important source of unsatisfactory liquid-based cytology. Although still being debate

  7. Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology

    DEFF Research Database (Denmark)

    Ikenberg, Hans; Bergeron, Christine; Schmidt, Dietmar;

    2013-01-01

    Pap cytology is known to be more specific but less sensitive than testing for human papillomavirus (HPV) for the detection of high-grade cervical intraepithelial neoplasia (CIN2+). We assessed whether p16/Ki-67 dual-stained cytology, a biomarker combination indicative of transforming HPV infections...

  8. Fine needle aspiration cytology of cervical lymph node involvement by ovarian serous borderline tumor.

    Science.gov (United States)

    Chen, Longwen; Butler, Kristina A; Bell, Debra A

    2016-01-01

    Serous borderline tumor (SBT) involving a cervical lymph node is extremely rare. In addition, fine needle aspiration (FNA) cytology of the involved cervical lymph node shares tremendous morphologic similarity with other low-grade papillary carcinomas. Thus, it can be easily misdiagnosed as metastatic carcinoma. A 42-year-old female had a history of bilateral SBT and postbilateral salpingo-oophorectomy. She presented with left cervical lymphadenopathy 6 months later. FNA cytology showed a low-grade papillary neoplasm with psammoma bodies. Needle core biopsy along with immunostains was diagnostic of cervical lymph node involvement (LNI) of SBT. although extremely rare, cervical LNI can be found in patients with SBTs. FNA cytology, sometimes, is indistinguishable from metastatic papillary adenocarcinoma. Cell block or needle core biopsy is essential to make the correct diagnosis. PMID:27563339

  9. Validity and reliability of using a self-lavaging device for cytology and HPV testing for cervical cancer screening: findings from a pilot study.

    Directory of Open Access Journals (Sweden)

    Heidi E Jones

    Full Text Available Self-sampling could increase cervical cancer screening uptake. While methods have been identified for human papillomavirus (HPV testing, to date, self-sampling has not provided adequate specimens for cytology. We piloted the validity and reliability of using a self-lavaging device for cervical cytology and HPV testing. We enrolled 198 women in New York City in 2008-2009 from three ambulatory clinics where they received cervical cancer screening. All were asked to use the Delphi Screener™ to self-lavage 1-3 months after clinician-collected index cytological smear (100 normal; 98 abnormal. Women with abnormal cytology results from either specimen underwent colposcopy; 10 women with normal results from both specimens also underwent colposcopy. We calculated sensitivity of self-collected cytology to detect histologically confirmed high grade lesions (cervical intraepithelial neoplasia, CIN, 2+; specificity for histology-negative (CIN 1 or lower, paired cytology negative, or a third cytology negative; and kappa for paired results. One hundred and ninety-seven (99.5% women self-collected a lavage. Seventy-five percent had moderate to excellent cellularity, two specimens were unsatisfactory for cytology. Seven of 167 (4% women with definitive results had CIN2+; one had normal and six abnormal cytology results with the self-lavage (sensitivity = 86%, 95% Confidence Interval, CI: 42, 100. The kappa for paired cytology was low (0.36; 95% CI: 0.25, 0.47 primarily due to clinician specimens with atypical squamous cells of undetermined significance (ASC-US and low grade squamous intraepithelial lesion (LSIL coded as normal using Screener specimens. However, three cases of HSIL were coded as ASC-US and one as normal using Screener specimens. Seventy-three women had paired high-risk HPV tests with a kappa of 0.66 (95% CI: 0.49, 0.84. Based on these preliminary findings, a larger study to estimate the performance of the Screener for co-testing cytology and

  10. Concordância entre citologia, colposcopia e histopatologia cervical Agreement between cytology, colposcopy and cervical histopathology

    Directory of Open Access Journals (Sweden)

    Letícia Maria Correia Katz

    2010-08-01

    Full Text Available OBJETIVO: avaliar a concordância da citologia convencional por Papanicolaou repetida no momento da colposcopia com os achados colposcópicos e a histopatologia. MÉTODOS: o estudo foi realizado no Laboratório Central de Saúde Pública do Estado de Pernambuco (LACEN, de janeiro a julho de 2008, em 397 mulheres com exame citopatológico alterado encaminhadas para avaliação colposcópica. No momento da colposcopia, repetiu-se a citologia em meio convencional, pesquisando-se os achados colposcópicos anormais. A nomenclatura citológica utilizada foi a de Bethesda e a histopatológica, da Organização Mundial de Saúde. A citologia no momento da colposcopia e a colposcopia foram comparadas entre si e com o resultado do histopatológico obtido por biópsia dirigida. A concordância entre os métodos foi avaliada pelo coeficiente Kappa (K, além do teste χ2 a um nível de significância de 5%. RESULTADOS: foi encontrada uma concordância fraca entre a citologia realizada no momento da colposcopia e a colposcopia, K=0,33 (IC95%=0,21-0,45 e entre a colposcopia e a histopatologia, K=0,35 (IC95%=0,39-0,51. Para a concordância entre citologia no momento da colposcopia e histopatologia, o Kappa foi de 0,41 (IC95%=0,29-0,530, considerado moderado. CONCLUSÕES: houve melhor concordância entre citologia e histopatologia do que entre colposcopia e citologia ou colposcopia e histopatologia.PURPOSE: to evaluate the agreement between conventional cytology using the Papanicolaou test, repeated at the time of colposcopy, with colposcopic and histopathological findings. METHODS: the study was carried out at the central public health laboratory of the state of Pernambuco between January and July, 2008, involving 397 women referred for colposcopic evaluation following an abnormal cervical smear test. Cytology was repeated at the time of colposcopy using conventional method, with particular attention being paid to the presence of abnormal colposcopic findings

  11. Cytological Analysis for Human Papillomavirus DNAs in Cervical Intraepithelial Neoplasia by In situ Hybridization

    OpenAIRE

    Nagai, Nobutaka; Takehara, Kazuhiro; Murakami, Takahiro; Ohama, Koso

    1994-01-01

    Human papillomavirus (HPV) type 16 and 18 DNAs are reported to be associated with uterine cervical cancer. In order to investigate the relationship between the presence of HPV DNA and cervical intraepithelial neoplasia (CIN), we attempted the cytological detection of HPV DNAs in uterine cervical smear samples. The samples included those of severe dysplasia and carcinoma in situ (CIS). They were analysed by DNA-DNA in situ hybridization using biotinylated HPV DNA probes.  The results of in sit...

  12. Cytological Analysis for Human Papillomavirus DNAs in Cervical Intraepithelial Neoplasia by In situ Hybridization

    OpenAIRE

    Nagai, Nobutaka; Takehara, Kazuhiro; Murakami, Takahiro; Ohama, Koso

    1994-01-01

    Human papillomavirus (HPV) type 16 and 18 DNAs are reported to be associated with uterine cervical cancer. In order to investigate the relationship between the presence of HPV DNA and cervical intraepithelial neoplasia (CIN), we attempted the cytological detection of HPV DNAs in uterine cervical smear samples. The samples included those of severe dysplasia and carcinoma in situ (CIS). They were analysed by DNA-DNA in situ hybridization using biotinylated HPV DNA probes.   The results of i...

  13. Hybrid capture vs. PCR screening of cervical human papilloma virus infections. Cytological and histological associations in 1270 women

    Directory of Open Access Journals (Sweden)

    Tsivilika Angeliki

    2010-02-01

    Full Text Available Abstract Background We evaluated two molecular methods of HPV detection and their correlation with cytological and histological diagnosis in a large sample of Greek women. Methods All women with liquid-based cytology performed at a University Hospital between 2000 and 2003 were included. The Hybrid Capture 2 (HC2 kit and in house Polymerase Chain Reaction (PCR were used for HPV DNA detection. Cervical biopsy was performed for women with ASCUS+ cytology, HPV detection, or abnormal colposcopy. Positive (PLR and negative (NLR likelihood ratios were calculated for cytology and HPV molecular testing for the prediction of CIN2 and greater histology. Results Of the 1270 women evaluated 241 (18.5% had abnormal cytology. Cytology diagnosed high-grade squamous intraepithelial lesion (HSIL or invasive carcinoma in 21(1.7% cases whereas 26 (2% women had CIN2+ or greater histology. PCR detected HPV in 397/1270 (31.3% and HC2 in 260/1270 (20.4% samples. Both molecular tests exhibited high reproducibility (Cohen's kappa value 0.691, 95% CI: 0.664 - 0.718. Positive likelihood ratios (PLR of 9.4, 3.8 and 3.4 and negative likelihood ratios of 0.13, 0.21, and 0 were noted for ≥ LSIL, any positive HC2 or any positive PCR-HPV testing, for predicting CIN2+ histology, respectively. All CIN 3+ lesions harbored high risk oncogenic HPV type infections. Conclusions HPV infection was found in a large proportion of this population and was associated with CIN 2/3 lesions and infiltrating carcinomas. Thin prep testing and HPV detection by HC2 or PCR performed very well with regards to identifying high grade lesions in an environment with experienced examiners.

  14. What women want. Women's preferences for the management of low-grade abnormal cervical screening tests: a systematic review

    DEFF Research Database (Denmark)

    Frederiksen, Maria Eiholm; Lynge, E; Rebolj, M

    2012-01-01

    cytology in primary cervical screening, the frequency of low-grade abnormal screening tests will double. Several available alternatives for the follow-up of low-grade abnormal screening tests have similar outcomes. In this situation, women's preferences have been proposed as a guide for management......Please cite this paper as: Frederiksen M, Lynge E, Rebolj M. What women want. Women's preferences for the management of low-grade abnormal cervical screening tests: a systematic review. BJOG 2011; DOI: 10.1111/j.1471-0528.2011.03130.x. Background If human papillomavirus (HPV) testing will replace....... Selection criteria Studies asking women to state a preference between active follow-up and observation for the management of low-grade abnormalities on screening cytology or HPV tests. Data collection and analysis Information on study design, participants and outcomes was retrieved using a prespecified form...

  15. Value of human papillomavirus typing for detection of anal cytological abnormalities

    Directory of Open Access Journals (Sweden)

    Livia Bravo Maia

    2013-01-01

    Full Text Available Purpose: The objective of this study was to evaluate anal cytology and human papillomavirus (HPV typing in patients with human immunodeficiency virus infection. Materials and Methods: Anal samples were collected from 61 patients (44 men and 17 women and analyzed by PapilloCheck test and conventional cytology. Results: Of all anal samples, 37.7% had cytological abnormalities, 47.54% were negative and 14.75% were unsatisfactory. High-risk HPV, multiple high-risk HPV and HPV 16 infection was detected in 91.13%, 78.26% and 47.82% of the samples with cytological abnormalities and in 47.54%, 6.89% and 3.44% of the negative samples, respectively. High-risk HPV infection was significantly more frequent in anal samples with cytological abnormalities than in negative samples (P = 0.0005, Fisher′s test, particularly multiple high-risk HPV infection (P < 0.0001 and HPV 16 infection (P = 0.0002. Conclusions: High-risk HPV, multiple high-risk HPV and HPV 16 infections are significantly associated with anal cytological abnormalities. Furthermore, the frequency of HPV infection in anal cytological samples suggests that high-risk HPV detection has high sensitivity, but low specificity for detection of anal cytological abnormalities, but multiple high-risk HPV typing and HPV 16 typing have a lower sensitivity and high specificity. Results suggest that HPV typing may be useful as an adjunct to cytology to screen patients for high-resolution anoscopy and biopsy.

  16. Human Papillomavirus Assays and Cytology in Primary Cervical Screening of Women Aged 30 Years and Above

    DEFF Research Database (Denmark)

    Rebolj, Matejka; Bonde, Jesper; Preisler, Sarah;

    2016-01-01

    In women aged ≥30 years, Human Papillomavirus testing will replace cytology for primary cervical screening. We compared Hybrid Capture 2 (HC2), cobas, CLART, and APTIMA HPV assays with cytology on 2869 SurePath samples from women undergoing routine screening at 30-65 years in Copenhagen, Denmark...... (positive test results without ≥CIN3) varied between 3.3% with cytology and 14.9% with cobas. All HPV assays led to significantly more false-positive tests, whereas compared to HC2 cobas and CLART were associated with a significantly higher and APTIMA with a significantly lower proportion. Detection of CIN1...... was particularly increased for the three DNA assays. With APTIMA combined with cytological triage, about 20% more women were referred for colposcopy than with cytology screening. With the three DNA assays, the increase was ≥50%. The number of women with repeated testing was twice as high with APTIMA and almost...

  17. Ultrasonography-Guided Fine Needle Aspiration Cytology of Cervical Lymphadenopathy: Comparative Study of the Cytological Adequacy Using of 21- and 23- Gauge Needles

    Energy Technology Data Exchange (ETDEWEB)

    Beak, Jin Wook; KIm, Dong Wook; Kim, Bo Mi [Busan Paik Hospital, Inje University School of Medicine, Busan (Korea, Republic of)

    2011-05-15

    To assess the difference in the cytological adequacy of ultrasonography (US)-guided fine needle aspiration cytology (US-FNAC) for cervical lymphadenopathy using 21 and 23 gauge needles. US-FNACs were separately performed on two cervical lymph nodes and in each patient using 21 gauge (group A) and 23 gauge (group B) needles, respectively. We used 4 grade classifications for the cytological analysis of each slide and compared the cytological adequacy of US-FNAC for cervical lymphadenopathy in the two groups. We performed US-FNACs on 58 cervical lymph nodes from 29 patients, with a cytological adequacy of 69.0%. The number of adequate cytological results was 19 (65.5%) in group A and 21 (72.4%) in group B, and the diagnostic adequacy did not differ significantly between the two groups (Mann-Whitney test, p = 0.574). There was no statistically significant difference in the cytological adequacy of US-FNAC for cervical lymphadenopathy according using of 21 and 23 gauge needles.

  18. Prevalence of human papillomavirus infection & cervical abnormalities in HIV-positive women in eastern India

    Directory of Open Access Journals (Sweden)

    Jaya Chakravarty

    2016-01-01

    Full Text Available Background & objectives: India has the third highest burden of HIV and highest number of cervical cancer in the world. A cross-sectional study was performed to determine the prevalence and types of human papillomavirus (HPV infection, and the factors associated with HPV infection and abnormal cervical cytology in HIV-positive women attending the Antiretroviral Therapy (ART Centre in a tertiary care hospital in eastern India. Methods: We screened 216 HIV- positive women with Papanicolau smear cytology and HPV testing. HPV DNA was detected by using consensus primers followed by sequencing. Results: Of the 216 HIV-positive women screened, 58 (26.85% were HPV-positive; 56 (25.9% were of high-risk (HR HPV type. The most prevalent HPV type was HPV-16 (7.9%; non 16 and 18 HPV types were present in 17.6 per cent patients. Age ≤ 35 yr [(OR, 2.56 (1.26-5.19], illiteracy [OR, 2.30 (1.19-4.46], rural residence [OR, 3.99 (1.27-12.56] and CD4 ≤350/µl [OR, 2.46 (1.26-4.83] were associated with increased risk of acquisition of HPV. One hundred thirty nine (74.33% patients had normal/ negative for intraepithelial lesions (NILM cytology, three (1.60% had atypical squamous cells of undetermined significance (ASCUS, 32 (17.11% had low-grade squamous intraepithelial lesions (LSIL, 10 (5.35% had high-grade squamous intraepithelial lesions (HSIL and three (1.60% had carcinoma cervix. WHO clinical Stage III and IV [OR, 2.83 (1.07-7.49] and CD4 ≤350/µl [OR, 2.84 (1.30-6.20] were risk factors for abnormal cytology. Interpretation &conclusions: Our study showed 26.85 per cent HPV positivity in HIV infected women in this region, with HPV-16 as the commonest genotype. Abnormal cervical cytology was seen in about 25 per cent women. Regular Pap smear screening as recommended by the National AIDS Control Organization will help in early detection of cervical abnormalities in HIV- positive women.

  19. Cervical cancer screening: on the way to a shift from cytology to full molecular screening.

    Science.gov (United States)

    Dijkstra, M G; Snijders, P J F; Arbyn, M; Rijkaart, D C; Berkhof, J; Meijer, C J L M

    2014-05-01

    Cytology-based nation-wide cervical screening has led to a substantial reduction of the incidence of cervical cancer in western countries. However, the sensitivity of cytology for the detection of high-grade precursor lesions or cervical cancer is limited; therefore, repeated testing is necessary to achieve program effectiveness. Additionally, adenocarcinomas and its precursors are often missed by cytology. Consequently, there is a need for a better screening test. The insight that infection with high-risk human papillomavirus (hrHPV) is the causal agent of cervical cancer and its precursors has led to the development of molecular tests for the detection of hrHPV. Strong evidence now supports the use of hrHPV testing in the prevention of cervical cancer. In this review, we will discuss the arguments in favor of, and concerns on aspects of implementation of hrHPV testing in primary cervical cancer screening, such as the age to start hrHPV-based screening, ways to increase screening attendance, requirements for candidate hrHPV tests to be used, and triage algorithms for screen-positive women. PMID:24445150

  20. Value of human papillomavirus typing for detection of anal cytological abnormalities

    OpenAIRE

    Livia Bravo Maia; Larissa Cardoso Marinho; Tânia Wanderley Paes Barbosa; Lara Franciele Ribeiro Velasco; Patrícia Godoy Garcia Costa; Fabiana Pirani Carneiro; Paulo Gonçalves de Oliveira

    2013-01-01

    Purpose: The objective of this study was to evaluate anal cytology and human papillomavirus (HPV) typing in patients with human immunodeficiency virus infection. Materials and Methods: Anal samples were collected from 61 patients (44 men and 17 women) and analyzed by PapilloCheck test and conventional cytology. Results: Of all anal samples, 37.7% had cytological abnormalities, 47.54% were negative and 14.75% were unsatisfactory. High-risk HPV, multiple high-risk HPV and HPV 16 infection was d...

  1. A study of metastatic lesions of cervical lymphadenopathy by fine needle aspiration cytology

    Directory of Open Access Journals (Sweden)

    Deepa Jethwani

    2015-07-01

    Conclusion: FNAC is a cost effective, reliable, rapid and inexpensive method for diagnosis of lymphadenopathy. Cytology of Metastatic lesions in cervical lymph nodes gives clue to nature and origin of tumors. [Int J Res Med Sci 2015; 3(7.000: 1697-1700

  2. Impact of technology on cytology outcome in cervical cancer screening of young and older women

    DEFF Research Database (Denmark)

    Rask, J; Lynge, E; Franzmann, M;

    2014-01-01

    Little is known about age-dependent variation in outcomes of cervical cytology with modern technologies. This population-based study evaluated age-dependent changes after routine implementation of ThinPrep and SurePath technology in two independent laboratories, and controlled for time trends in ...

  3. Is it necessary to repeat cervical cytology at the tie of a colposcopy? É necessário repetir a citologia cervical no momento da colposcopia?

    Directory of Open Access Journals (Sweden)

    Leticia Maria Correia Katz

    2012-09-01

    Full Text Available OBJECTIVES: to evaluate whether repeating Papanicolaou smear testing at the time of colposcopy improves agreement between cytology and histopathology in the diagnosis of cervical cancer. METHODS: this cross-sectional study included 397 women referred for colposcopic evaluation following an abnormal cervical smear test. The cytology was repeated at the time of the colposcopy using a conventional medium. The two cytology tests were compared with each other and with the histopathological findings obtained by colposcopy-directed biopsy. The 2001 Bethesda system and the WHO 1994 classification were used for reporting cytology and histology results. The kappa coefficient was used to determine the agreement between methods. RESULTS: the comparison between the initial cytology findings and cytology performed at the time of colposcopy revealed a kappa of 0.297 (95%CI: 0.235-0.359, indicating a fair degree of agreement. When the results of the initial cytology were compared with histopathology, a kappa of 0.261, considered to represent a fair degree of agreement, was obtained (95%CI: 0.181-0.340. A kappa of 0.408, considered to represent moderate agreement, was found when the second cytology findings were compared with the histopathology (95%CI: 0.332-0.485. CONCLUSIONS: the agreement between cytology and histology improved when cytology was repeated at the time of colposcopy, following an initial abnormal test.OBJETIVOS: avaliar se a repetição do Papanicolaou no momento da colposcopia melhora a concordância entre os métodos diagnósticos (citologia e histopatologia do câncer do colo do útero. MÉTODOS: o estudo foi realizado no Laboratório Central de Saúde Pública do Estado de Pernambuco (LACEN/PE, de janeiro a julho de 2008, em 397 mulheres com exame citopatológico alterado encaminhadas para avaliação colposcópica. No momento da colposcopia, repetiu-se a citologia, em meio convencional. As duas citologias foram comparadas entre si e com o

  4. P16INK4a: a potential diagnostic adjunct for prediction of high-grade cervical lesions in liquid-based cytology: with HPV testing and histological correlation.

    Science.gov (United States)

    Wong, Y P; Abdul Raub, S H; Mohd Dali, A Z H; Kassim, F; Visvalingam, V; Zakaria, Z; Kamaluddin, M A; Noor Akmal, S

    2016-08-01

    Human papillomavirus (HPV) is a necessary cause of cervical cancer and its precursors. Increased expression of high-risk hrHPV viral oncogenes in abnormal cells might increase the expression of p16INK4a. We aimed to determine the role of p16INK4a in detecting hrHPV-transformed epithelial cells in liquid-based cervical cytology, and compared the results with hrHPV DNA testing by realtime polymerase chain reaction (RT-PCR). Fifty-seven cytological samples were tested for p16INK4a immunomarker and hrHPV DNA. Test performance of both tests was determined by comparing sensitivity, specificity and predictive values using available histological follow-up data as gold standard. Of 57 samples, 36 (63.2%) showed immunoreactivity for p16INK4a and 43 (75.4%) were hrHPV-infected. A fairly low concordance rate (k = 0.504) between p16INK4a immunolabelling and hrHPV DNA status was noted. For prediction of cervical intraepithelial neoplasia (CIN) II and worse lesions, p16INK4a had a sensitivity and specificity of 93.5% and 60%; whereas hrHPV DNA testing had a sensitivity and specificity of 100% and 20%. Dual testing by combining p16INK4a and hrHPV showed sensitivity and specificity of 100% and 33.3%. In conclusion, p16INK4a is useful in predicting severity of the cytological abnormalities. Although p16INK4a is more specific but less sensitive than hrHPV in detecting high-grade cervical lesions, a combination of both tests failed to demonstrate significant improvement in diagnostic sensitivity, specificity and predictive value. Larger-scale prospective studies are required to assess further whether this biomarker should be routinely used as primary screening tool independently or in combination with hrHPV testing to improve diagnostic accuracy in cervical cytology. PMID:27568665

  5. HPV-DNA testing and Ki-67 immunocytochemistry in liquid based cervical cytology in prostitute women

    Directory of Open Access Journals (Sweden)

    Esin ATİK

    2006-09-01

    Full Text Available HPV causes several changes in the function of host genes, and these interactions cause deregulation of the cell cycle manifested by abnormal expression of cell cycle associated proteins, such as Ki-67. The detection of Ki-67 can play a role in screening and diagnosis of HPV infection with risk of progression towards dysplasia and carcinoma. To show this relation in prostitute women, cervical cells were collected in the PapSpin Collection Fluid. A starting volume of 1000 µl for each sample, and a 200 µl cell suspension were used to prepare each sample for thin layer liquid based cytology and then they were stained by Papanicolaou method. The cytological results were classified according to the Bethesda 2001 system. From the remaining cell suspension of 800 µl, a 400 µl sample was used for HPV-DNA detection by PCR, a 50 µl alliquot was used to make thin layer preparations for immunocytochemistry. Single antigen staining was performed with Ki-67 protein. Cells were considered immunopositive if the nuclei were stained. All cells in one high power field (x400 were counted, and the fraction of immunopositive cells on the slide was calculated. This fraction was expressed as the number of positive cells per 1000 cells to facilitate comparisons of differential cell counts. HPV types 6 and 32 in the study, and HPV types 6 and 51 in the control group were detected. The mean Ki-67 values were 2.7±1.2 and 3.6±4.1 in HPV positive and negative cases respectively. There was a positive correlation only with nuclear changes and HPV positivity (x2=28.8, p<0.001. There was not any significant correlation between HPV or Ki-67 and leukocytosis. An association with HPV and contraception, smoking, and concurrent genital infection was not found. The prevalence of HPV types in different geographical locations and races may indicate different etiologies of cervical cancer. Our results suggest that Ki-67 immunocytochemistry is not useful as a surrogate marker for

  6. Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL.

    Science.gov (United States)

    Halliwell, Diane E; Morais, Camilo L M; Lima, Kássio M G; Trevisan, Julio; Siggel-King, Michele R F; Craig, Tim; Ingham, James; Martin, David S; Heys, Kelly A; Kyrgiou, Maria; Mitra, Anita; Paraskevaidis, Evangelos; Theophilou, Georgios; Martin-Hirsch, Pierre L; Cricenti, Antonio; Luce, Marco; Weightman, Peter; Martin, Francis L

    2016-01-01

    Cervical cancer remains a major cause of morbidity and mortality among women, especially in the developing world. Increased synthesis of proteins, lipids and nucleic acids is a pre-condition for the rapid proliferation of cancer cells. We show that scanning near-field optical microscopy, in combination with an infrared free electron laser (SNOM-IR-FEL), is able to distinguish between normal and squamous low-grade and high-grade dyskaryosis, and between normal and mixed squamous/glandular pre-invasive and adenocarcinoma cervical lesions, at designated wavelengths associated with DNA, Amide I/II and lipids. These findings evidence the promise of the SNOM-IR-FEL technique in obtaining chemical information relevant to the detection of cervical cell abnormalities and cancer diagnosis at spatial resolutions below the diffraction limit (≥0.2 μm). We compare these results with analyses following attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy; although this latter approach has been demonstrated to detect underlying cervical atypia missed by conventional cytology, it is limited by a spatial resolution of ~3 μm to 30 μm due to the optical diffraction limit. PMID:27406404

  7. Anal cytology in women with cervical intraepithelial or invasive cancer: interobserver agreement

    Directory of Open Access Journals (Sweden)

    Sandra A. Heráclio

    2015-10-01

    Full Text Available ABSTRACTIntroduction:Incidence rates of anal cancer have been rising worldwide in the last 20 years. Due to embryological, histological and immunohistochemical similarities between the anal canal and the cervix, routine screening with anal cytology for precursor lesions in high-risk groups has been adopted. Objective: To determine interobserver agreement for the diagnosis of anal neoplasia by anal cytology.Material and methods:A cross-sectional observational study was conducted in 324 women with cervical intraepithelial or invasive cancers, for screening of anal cancer, from December 2008 to June 2009. Three hundred twenty-four cytological samples were analyzed by three cytopathologists. Cytological evaluation was based on the revised Bethesda terminology; samples were also classified into negative and positive for atypical cells. We calculated the kappa statistic with 95% confidence interval (95% CI to assess agreement among the three cytopathologists.Results:Interobserver agreement in the five categories of the Bethesda terminology was moderate (kappa for multiple raters: 0.6. Agreement among cytopathologists 1, 2 and 3 with a consensus diagnosis was strong (kappa: 0.71, 0.85 and 0.82, respectively.Conclusion:Interobserver agreement in anal cytology was moderate to strong, indicating that cervical cytomorphological criteria are reproducible also in anal samples.

  8. Quantitative Detection of Screening for Cervical Lesions with ThinPrep Cytology Test

    Institute of Scientific and Technical Information of China (English)

    Hong-xin ZHANG; Yi-min SONG; Su-hong LI; Yu-hui YIN; Dong-ling GAO; Kui-sheng CHEN

    2010-01-01

    OBJECTIVE To investigate the available parameters in gynecological screening for cervical lesions by liquid-based cytology technology (ThinPrep Cytology Test, TCT) and The Bethesda System (TBS), also with computer image analysis. METHODS With application of the image analysis system, all grades of cervical lesion cells were detected quantitatively and sorted in atypical squamous cells of undetermined significance (ASCUS), atypical squamous cells-cannot exclude HSIL (ASC-H), low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL) and cervical squamous cell carcinoma (SCC) with the mean optical density (MOD), average grey (AG), positive units (PU), and nucleus to cytoplasmic ratio (N:C). Differences between each group of cells were compared and analyzed statistically.RESULTS Apart from four stereologic parameters in LSIL and HSIL groups there were no differences among them, in the other groups, there was statistically significant in differences between MOD, AG and PU values. Differences between them in the ratio of nucleus to cytoplasm were highly statistically signifi cant. CONCLUSION Stereological indexes may serve as a screening tool for cervical lesions. The image analysis system is expected to become a new means of cytological assisted diagnosis.

  9. Abnormal cervical smears in the unchanged uterine cervix: difficulties in the screening, diagnosis, and treatment of cervical intraepithelial neoplasias and microinvasive cancer

    Directory of Open Access Journals (Sweden)

    L. I. Korolenkova

    2011-01-01

    Full Text Available The author analyzes an algorithm for identifying and treating w omen with cervical intraepithelial neoplasias (CIN and microinv asive cancer of the cervix uteri in cases of the hidden area of transformation and in the absence of visible cervical changes. There are excep- tional difficulties of making the diagnosis of epithelial damages due to the incomplete reproducibility of cytological abnormal ities and the low informative value of a histological study of scrapes from the cervical canal. To avoid hypodiagnosis, it is justifiable to prefer human papillomavirus testing (Hybrid Capture 2 (HC2 to repeat smears for the choice of a management tactic. Conization is recommend ed as a diagnostic and/or therapeutic procedure when the viral load is high in over 35-year-old patients with abnormal smears anda hidden transformation area.

  10. Human papillomavirus genotypes in women with cervical cytological abnormalities from an area with high incidence of cervical cancer Genotipos de virus papiloma humano en mujeres con alteraciones citológicas cervicales de un área con alta incidencia de cáncer cervical

    Directory of Open Access Journals (Sweden)

    Gerardo Daniel Deluca

    2004-02-01

    Full Text Available It has been well demonstrated the relationship between the infection with high-risk human papillomavirus (HPVs genotypes and cervical cancer. In Northeastern Argentina a high incidence of this pathology has been described and therefore a high prevalence of HPV infection is expected. In order to identify HPV genotypes associated with malignant and pre-malignant cervical lesions present in the area, 53 ecto-endo cervical cell specimens obtained from women with cytohistological alterations were studied by a PCR-RFLP technique. Out of 53 patients, 34 (64.2% were positive for HPV infection, being HPV-16 (32.3% the most frequently found genotype, followed by HPV-58 (14.7%, -6, -18 and -45 (5.9%, -33, -52, -53, -54, -56, -66, -MM4 and -LVX100 (2.9%. Also 5 cases of infection caused by multiple genotypes were found, which corresponded to 14.7% of the positive cases. Results indicate that besides HPV-16 and -18, the most prevalent high-risk HPV genotypes worldwide, others like -45 and -58 as well as co-infection cases are frequent between women of Northeastern Argentina, and a particular attention should be paid to this circumstance because it could be an epidemiological feature of regional importance and a useful information for a future vaccination program.La relación entre la infección por los virus papiloma humanos (HPVs de alto riesgo y el cáncer de cuello de útero ha sido bien demostrada. En el Nordeste de Argentina se observa una alta incidencia de esta patología y en consecuencia se estima una alta prevalencia de infección por HPV. A fin de identificar los genotipos de HPV presentes en el área, asociados a casos de lesiones malignas y premalignas de cuello de útero, se estudiaron 53 muestras ecto-endo cervicales de mujeres con alteraciones citohistológicas residentes permanentes de las ciudades de Resistencia y Corrientes. De las 53 pacientes estudiadas, 34 resultaron positivas para HPV (64.2%, correspondiendo la mayor frecuencia a HPV

  11. Cytological patterns of cervical pap smears with histopathological correlation

    Directory of Open Access Journals (Sweden)

    Bhagya Lakshmi Atla

    2015-08-01

    Conclusion: Pap smear is simple, inexpensive and can be performed in the outpatient department. Hence, it should be recommended routinely as a method of improving reproductive health, early detection of premalignant and malignant cervical lesions. [Int J Res Med Sci 2015; 3(8.000: 1911-1916

  12. [Risk factors for uterine cervical cancer according to results of VIA, cytology and cervicography].

    Science.gov (United States)

    dos Anjos, Saiwori de Jesus Silva Bezerra; Vasconcelos, Camila Teixeira Moreira; Franco, Eugênio Santana; de Almeida, Paulo César; Pinheiro, Ana Karina Bezerra

    2010-12-01

    This study aimed to evaluate the association between risk factors for uterine cervical neoplasms and cervical lesions by HPV by comparison of the visual inspection with acetic acid (VIA), cytology and cervicography results. A prevalence research was made with 157 women in a health center of Fortaleza in the period of June to September 2006. The SPSS program was used to codify the data. Inferences were made through statistical tests (chi2 = chi square and LR = likelihood ratio). The VIA, cervicography and cytology obtained 43.3%, 10.19% and 3.2% of altered results. The variables with important association to cervical lesions in the VIA were: aged less than 20 years old (p = 0.0001); one or more partners in the last three months (p = 0.015); use of contraceptives (p = 0.0008); presence of vaginal discharge (p = 0.0001) and moderate or accentuated inflammatory process (p = 0.0001). In the cytology: low instructional level (p = 0.0001) and high pH (p = 0.001). It wasn't found any significant association in the cervicography.

  13. Psychosocial impact of alternative management policies for low-grade cervical abnormalities: results from the TOMBOLA randomised controlled trial.

    Directory of Open Access Journals (Sweden)

    Linda Sharp

    Full Text Available BACKGROUND: Large numbers of women who participate in cervical screening require follow-up for minor cytological abnormalities. Little is known about the psychological consequences of alternative management policies for these women. We compared, over 30-months, psychosocial outcomes of two policies: cytological surveillance (repeat cervical cytology tests in primary care and a hospital-based colposcopy examination. METHODS: Women attending for a routine cytology test within the UK NHS Cervical Screening Programmes were eligible to participate. 3399 women, aged 20-59 years, with low-grade abnormal cytology, were randomised to cytological surveillance (six-monthly tests; n = 1703 or initial colposcopy with biopsies and/or subsequent treatment based on colposcopic and histological findings (n = 1696. At 12, 18, 24 and 30-months post-recruitment, women completed the Hospital Anxiety and Depression Scale (HADS. A subgroup (n = 2354 completed the Impact of Event Scale (IES six weeks after the colposcopy episode or first surveillance cytology test. Primary outcomes were percentages over the entire follow-up period of significant depression (≥ 8 and significant anxiety (≥ 11; "30-month percentages". Secondary outcomes were point prevalences of significant depression, significant anxiety and procedure-related distress (≥ 9. Outcomes were compared between arms by calculating fully-adjusted odds ratios (ORs for initial colposcopy versus cytological surveillance. RESULTS: There was no significant difference in 30-month percentages of significant depression (OR = 0.99, 95% CI 0.80-1.21 or anxiety (OR = 0.97, 95% CI 0.81-1.16 between arms. At the six-week assessment, anxiety and distress, but not depression, were significantly less common in the initial colposcopy arm (anxiety: 7.9% vs 13.4%; OR = 0.55, 95% CI 0.38-0.81; distress: 30.6% vs 39.3%, OR = 0.67 95% CI 0.54-0.84. Neither anxiety nor depression differed between arms at subsequent time

  14. Cervical cytology as a diagnostic tool for female genital schistosomiasis: Correlation to cervical atypia and Schistosoma polymerase chain reaction

    Directory of Open Access Journals (Sweden)

    Pavitra Pillay

    2016-01-01

    Full Text Available Background: Female genital schistosomiasis (FGS is a tissue reaction to lodged ova of Schistosoma haematobium in the genital mucosa. Lesions can make the mucosa friable and prone to bleeding and discharge. Women with FGS may have an increased risk of HIV acquisition, and FGS may act as a cofactor in the development of cervical cancer. Objectives: To explore cytology as a method for diagnosing FGS and to discuss the diagnostic challenges in low-resource rural areas. The correlation between FGS and squamous cell atypia (SCA is also explored and discussed. Cytology results are compared to Schistosoma polymerase chain reaction (PCR in vaginal lavage and urine and in urine microscopy. Materials and Methods: In a clinical study, 394 women aged between 16 and 23 years from rural high schools in KwaZulu-Natal, South Africa, underwent structured interviews and the following laboratory tests: Cytology Papanicolaou (Pap smears for S. haematobium ova and cervical SCA, real-time PCR for Schistosoma-specific DNA in vaginal lavage and urine samples, and urine microscopy for the presence of S. haematobium ova. Results: In Pap smears, S. haematobium ova were detected in 8/394 (2.0%. SCA was found in 107/394 (27.1%, seven of these had high-grade squamous intraepithelial lesion (HSIL. Schistosoma specific DNA was detected in 38/394 (9.6% of vaginal lavages and in 91/394 (23.0% of urines. Ova were found microscopically in 78/394 (19.7% of urines. Conclusion: Schistosoma PCR on lavage was a better way to diagnose FGS compared to cytology. There was a significant association between S. haematobium ova in Pap smears and the other diagnostic methods. In low-resource Schistosoma-endemic areas, it is important that cytology screeners are aware of diagnostic challenges in the identification of schistosomiasis in addition to the cytological diagnosis of SCA. Importantly, in this study, three of eight urines were negative but showed Schistosoma ova in their Pap smear, and

  15. Serum alpha-antitrypsin in patients with lung cancer or abnormal sputum cytology

    Energy Technology Data Exchange (ETDEWEB)

    Harris, C.C.; Cohen, M.H.; Connor, R.; Primack, A.; Saccomanno, G.; Talamo, R.C.

    1976-10-01

    Scrum alpha/sub 1/-antitrypsin Pi types and trypsin inhibitory capacity (TIC) were measured in 72 patients with lung cancer and in 196 patients with abnormal sputum cytology but no clinical evidence of lung cancer to determine if a genetic deficiency of alpha/sub 1/-antitrypsin (AAT) predisposes to lung cancer. The distributions of Pi types in these two groups of patients and healthy adults are similar. Serum TIC and AAT concentrations are elevated in lung cancer patients. However, patients with abnormal sputum cytology and no clinical lung cancer have normal levels of serum TIC and AAT. A genetic deficiency of AAT probably does not produce a state of increased susceptibility to the carcinogenic effects of respiratory carcinogens such as tobacco smoke.

  16. [Health technology assessment report. Use of liquid-based cytology for cervical cancer precursors screening].

    Science.gov (United States)

    Ronco, Guglielmo; Confortini, Massimo; Maccallini, Vincenzo; Naldoni, Carlo; Segnan, Nereo; Sideri, Mario; Zappa, Marco; Zorzi, Manuel; Calvia, Maria; Giorgi Rossi, Paolo

    2012-01-01

    OBJECTIVE OF THE PROJECT: Purpose of this Report is to evaluate the impact of the introduction of liquid-based cytology (LBC) in cervical cancer screening in terms of efficacy, undesired effects, costs and implications for organisation. EFFICACY AND UNDESIRED EFFECTS: LBC WITH MANUAL INTERPRETATION: The estimates of cross-sectional accuracy for high-grade intraepithelial neoplasia (CIN2 or more severe and CIN3 or more severe) obtained by a systematic review and meta-analysis published in 2008 were used. This review considered only studies in which all women underwent colposcopy or randomised controlled trials (RCTs) with complete verification of test positives. A systematic search of RCTs published thereafter was performed. Three RCTs were identified. One of these studies was conducted in 6 Italian regions and was of large size (45,174 women randomised); a second one was conducted in another Italian region (Abruzzo) and was of smaller size (8,654 women randomised); a third RCT was conducted in the Netherlands and was of large size (89,784 women randomised). No longitudinal study was available. There is currently no clear evidence that LBC increases the sensitivity of cytology and even less that its introduction increases the efficacy of cervical screening in preventing invasive cancers. The Italian randomised study NTCC showed a decrease in specificity, which was not observed in the other two RCTs available. In addition, the 2008 meta-analysis observed a reduction - even if minimal - in specificity just at the ASC-US cytological cut-off, but also a remarkable heterogeneity between studies. These results suggest that the effect of LBC on specificity is variable and plausibly related to the local style of cytology interpretation. There is evidence that LBC reduces the proportion of unsatisfactory slides, although the size of this effect varies remarkably. LBC WITH COMPUTER-ASSISTED INTERPRETATION: An Australian study, based on double testing, showed a statistically

  17. [Satisfactory cervical cytology. Circular exocervical cytologic smears against longitudinal exocervical smears].

    Science.gov (United States)

    Ramos-Ortega, Gregorio; Díaz-Hernández, Maritza Consuelo; Rodríguez-Moctezuma, José Raymundo; Domínguez-Gómez, Francisco Guillermo

    2014-01-01

    INTRODUCCIÓN: en el Instituto Mexicano del Seguro Social, la muestra del frotis del cuello uterino se extiende en el portaobjetos mediante movimientos circulares (técnica habitual) desde el año de 1962. En 2006, la Secretaría de Salud estableció que el extendido exocervical se realizara de forma longitudinal (técnica convencional). El objetivo de la investigación que se presenta fue evaluar la eficacia y calidad de la técnica de extendido exocervical habitual, convencional y una nueva que se propone. MÉTODOS: estudio cuasiexperimental en mujeres que solicitaron prueba citológica cervical. Las variables analizadas fueron técnica de extendido, cobertura celular y calidad de la muestra exocervical. Se conformaron grupos según la técnica que se aplicó. Los estudios citológicos fueron procesados con técnica de Papanicolaou y los resultados fueron informados por tres observadores conforme al sistema Bethesda. Se utilizó prueba Z, ?2, Anova y riesgo relativo.

  18. 液基细胞学筛查宫颈癌临床研究应用%Liquid-based cytology screening for cervical cancer of clinical research and application

    Institute of Scientific and Technical Information of China (English)

    李彩华

    2010-01-01

    Objective To make a comparative study between the Liquid-based cervical cytology and the Papanicolaou stain cytology,to evaluate the sensitivity of Liquid-based cytology to cervical pathology screening and its clinical value.Methods To apply the cytological analysis on 511 patients in two ways by using the TBS standards to let the two results be analyzed and compared with each other.Results Among the patients who are abnormal both in the Liquid-based cytology and the Papanicolaou cytology,the positive rate of the Liquid-based cytology is obviously higher than the Papanicolaou cytology' s,compared to the tissue biopsy.Conclusion Liquid-based cytology has a higher sensitivity compared with Papanicolaou cytology.It be can able to enhance the rate of detecting,thus achieve the goal of early detection,diagnosis and treatment.%目的 将宫颈液基细胞学与巴氏细胞学染色进行比较研究,评价液基细胞学对宫颈病变筛查的敏感性与临床价值.方法 对511例患者经过两种方法进行细胞学分析采用TBS标准,将其结果进行比较分析.结果 在液基细胞学与巴氏细胞学两种方法均见异常的患者中,以组织学活检做对照,液基细胞学阳性率明显高于巴氏细胞学.结论 液基细胞学与巴氏细胞学诊断相比,具有更高的敏感性,能提高检出率,从而使患者得到早发现、早诊断及早治疗.

  19. COMPARISON BETWEEN DNA DETECTION TEST OF HUMAN PAPILLOMA VIRUS AND THE HYBRID CAPTURE SYSTEM WITH CYTOLOGY IN CERVICAL SMEARS.

    Directory of Open Access Journals (Sweden)

    Maristela Gabriel

    2006-06-01

    Full Text Available The Human Papilloma Virus (HPV has significant importance in invasive cervical cancer etiology of and its precursory injuries. Thus, it is necessary the application of molecular methodologies for the precocious diagnosis of this infection. This research has evaluated the effectiveness of Hybrid Capture System (HCS in detecting pathogenic types of HPV. HCS was compared with empirical established cytological criteria in specimens with cellular alterations that were suggestive of HPV infection. The presence of HPV was also evaluated in cytological normal specimens with inflammation and Cervical Intraepithelial Neoplasia (CIN. One hundred and eight women, suspected of HPV infection (clinical or cytological suspicion, were selected. Results demonstrated that both methods were complementary, since Hybrid Capture confirmed results of the cytology. The two methods may be used together to improve the diagnosis. Furthermore, the use of Hybrid Capture test in HPV diagnosis allows the identification of the risk that the virus proportionate.

  20. A STUDY OF HISTOPATHOLOGICAL CORRELATION WITH FINE NEEDLE ASPIRATION CYTOLOGY OF CERVICAL LYMPHADENOPATHY

    Directory of Open Access Journals (Sweden)

    Bobby Duarah

    2016-07-01

    Full Text Available BACKGROUND Cervical lymphadenopathy is a common manifestation in our country where incidence of tuberculosis and oral cancer is fairly high, so the proper and early diagnosis of lymphadenopathy is very important for early and effective treatment. AIM The present study was undertaken in the Department of Pathology, Assam Medical College and Hospital, for a period of one year with a view to correlate the cytological diagnosis with histopathology. MATERIALS AND METHODS A series of 100 cases with clinical presentation of palpable lymph node at any part of the cervical region irrespective of age and sex attending the different OPDs of this institution were taken. FNAC was done as an initial step for diagnosis. The results were confirmed by incisional/excisional biopsy afterwards. RESULT Out of 100 cases, there were 74 cases of benign lesions (74% and 26 cases of malignant lesions (26% proven histopathologically. The peak incidence of age group suffering from the disease was 21-30 years (25% followed by 11-20 years (24%. Majority were Tubercular (40% followed by Inflammatory (34%, Metastasis (16%, and Lymphoma (10%. All the cases (Benign and Malignant presented with neck swelling (100%. It was seen that the Jugulodigastric, Posterior triangle, and the submandibular groups of lymph nodes were the most commonly involved groups with a percentage being 41%, 21%, and 13% respectively. Out of a total of 74 histologically proved benign cases, cytology could diagnose 73 cases (98.7%. The percentage of accuracy was 98.7%. Also, the cytological diagnosis could be obtained in 24 out of 26 cases with malignant lesions when correlated with histopathology. The percentage of accuracy was 92.3%. It was seen that the overall accuracy rate of aspiration cytology in the diagnosis of benign lesions was 98.7% and that of malignant lesions was 92.3% against 100% accuracy with histopathology. The overall accuracy of aspiration cytology is 97%. CONCLUSION Though

  1. Application of Bethesda system for conventional cervical cytology: a study of 340 cases

    Directory of Open Access Journals (Sweden)

    Lalji G. Valiya

    2015-09-01

    Results: Overall incidence of unsatisfactory smears was 9.4%, of squamous cell abnormality was 4.1% and of glandular cell abnormality was 1.2%. Highest frequency of epithelial cell abnormalities (squamous as well as glandular was seen in 61-70 years age-group women (35.71% and in women presented with complaints of post-menopausal bleeding (22.5%. Conclusions: The study had shown a relatively low prevalence of epithelial abnormalities in cervical smears. The 2001 Bethesda system seems to reduce the number of cervical smear diagnoses of Atypical Squamous Cells (ASC, without causing any impairment in the diagnosis of High grade Squamous Intraepithelial Lesion (HSIL thus decreasing the number of unnecessary interventions. [Int J Res Med Sci 2015; 3(9.000: 2252-2258

  2. Mycoplasma genitalium infection is associated with microscopic signs of cervical inflammation in liquid cytology specimens.

    Science.gov (United States)

    Dehon, Patricia M; McGowin, Chris L

    2014-07-01

    Cervicitis is a common clinical finding often attributed to sexually transmitted infections (STIs), but no etiologic agent is identified in the majority of cases. In this study, we comparatively assessed inflammation among the common infectious etiologies of cervicitis and assessed the potential value of liquid cytology specimens for predicting STIs. Among 473 Louisiana women at low risk for acquiring STIs, the prevalences of Mycoplasma genitalium, Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis in liquid-based cytology specimens were 1.5, 2.1, 0.6, and 4.4%, respectively. N. gonorrhoeae and human papillomavirus 18 (HPV18) infections were significantly more common among subjects infected with M. genitalium. Using direct microscopy, we observed significant increases in leukocyte infiltrates among subjects with monoinfections with M. genitalium or C. trachomatis compared to women with no detectable STIs. Inflammation was highest among subjects with M. genitalium. Using a threshold of ≥ 2 leukocytes per epithelial cell per high-powered field, the positive predictive values for M. genitalium, C. trachomatis, N. gonorrhoeae, and T. vaginalis were 100, 70, 67, and 20%, respectively. Several novel M. genitalium genotypes were identified, all of which were predicted to be susceptible to macrolide antibiotics, suggesting that different strains may circulate among low-risk women and that macrolide resistance is substantially lower than in high-risk populations. This study highlights the capacity of M. genitalium to elicit cervical inflammation and, considering the strong epidemiologic associations between M. genitalium and human immunodeficiency virus (HIV), provides a potential mechanism for acquisition and shedding of HIV via chronic leukocyte recruitment to the cervical mucosa. PMID:24759719

  3. Bonafide, type-specific human papillomavirus persistence among HIV-positive pregnant women: predictive value for cytological abnormalities, a longitudinal cohort study

    Directory of Open Access Journals (Sweden)

    Angela RI Meyrelles

    2016-02-01

    Full Text Available This study investigated the rate of human papillomavirus (HPV persistence, associated risk factors, and predictors of cytological alteration outcomes in a cohort of human immunodeficiency virus-infected pregnant women over an 18-month period. HPV was typed through L1 gene sequencing in cervical smears collected during gestation and at 12 months after delivery. Outcomes were defined as nonpersistence (clearance of the HPV in the 2nd sample, re-infection (detection of different types of HPV in the 2 samples, and type-specific HPV persistence (the same HPV type found in both samples. An unfavourable cytological outcome was considered when the second exam showed progression to squamous intraepithelial lesion or high squamous intraepithelial lesion. Ninety patients were studied. HPV DNA persistence occurred in 50% of the cases composed of type-specific persistence (30% or re-infection (20%. A low CD4+T-cell count at entry was a risk factor for type-specific, re-infection, or HPV DNA persistence. The odds ratio (OR was almost three times higher in the type-specific group when compared with the re-infection group (OR = 2.8; 95% confidence interval: 0.43-22.79. Our findings show that bonafide (type-specific HPV persistence is a stronger predictor for the development of cytological abnormalities, highlighting the need for HPV typing as opposed to HPV DNA testing in the clinical setting.

  4. Bonafide, type-specific human papillomavirus persistence among HIV-positive pregnant women: predictive value for cytological abnormalities, a longitudinal cohort study

    Science.gov (United States)

    Meyrelles, Angela RI; Siqueira, Juliana D; dos Santos, Pâmela P; Hofer, Cristina B; Luiz, Ronir R; Seuánez, Héctor N; Almeida, Gutemberg; Soares, Marcelo A; Soares, Esmeralda A; Machado, Elizabeth S

    2016-01-01

    This study investigated the rate of human papillomavirus (HPV) persistence, associated risk factors, and predictors of cytological alteration outcomes in a cohort of human immunodeficiency virus-infected pregnant women over an 18-month period. HPV was typed through L1 gene sequencing in cervical smears collected during gestation and at 12 months after delivery. Outcomes were defined as nonpersistence (clearance of the HPV in the 2nd sample), re-infection (detection of different types of HPV in the 2 samples), and type-specific HPV persistence (the same HPV type found in both samples). An unfavourable cytological outcome was considered when the second exam showed progression to squamous intraepithelial lesion or high squamous intraepithelial lesion. Ninety patients were studied. HPV DNA persistence occurred in 50% of the cases composed of type-specific persistence (30%) or re-infection (20%). A low CD4+T-cell count at entry was a risk factor for type-specific, re-infection, or HPV DNA persistence. The odds ratio (OR) was almost three times higher in the type-specific group when compared with the re-infection group (OR = 2.8; 95% confidence interval: 0.43-22.79). Our findings show that bonafide (type-specific) HPV persistence is a stronger predictor for the development of cytological abnormalities, highlighting the need for HPV typing as opposed to HPV DNA testing in the clinical setting. PMID:26872340

  5. 围绝经期宫颈病变所致阴道流血%Perimenopausal abnormal vaginal bleeding caused by cervical lesions

    Institute of Scientific and Technical Information of China (English)

    王登凤; 张国楠

    2012-01-01

    围绝经期妇女中,不规则阴道流血是一个最常见、也是最易被忽视的症状,宫颈病变是其主要原因之一,主要包括了宫颈炎、宫颈炎症相关疾病、宫颈上皮内瘤变、宫颈癌、宫颈肌瘤、宫颈结核、宫颈妊娠等,可以通过妇科查体、宫颈脱落细胞学检查、宫颈活检等简单手段在短时间内得到确诊.%For perimenopausal women, abnormal vaginal bleeding is the most common symptom, which is also the most neglected one. In various causes, cervical lesion is the one which can be diagnosed by some simple methods, e. g. gynecological examination , cervical cytology, and cervical biopsy. Cervical lesions in this review include: cervicitis, cervicitis related disease, cervical intraepithelial neoplasia, cervical cancer, cervical myoma, tuberculosis of cervix, cervical pregnancy, etc.

  6. Evaluation of adjunctive HPV testing by Hybrid Capture II® in women with minor cytological abnormalities for the diagnosis of CIN2/3 and cost comparison with colposcopy

    Directory of Open Access Journals (Sweden)

    Kyi May S

    2003-09-01

    Full Text Available Abstract Background As a proportion of high grade cervical intraepithelial neoplasia (CIN2/3 are associated with equivocal cervical smears, which show borderline or mild dyskaryosis, follow up with repeat smears, colposcopy and biopsy is required. Since infection with oncogenic Human Papilloma Virus (HR HPV has been found to be associated with the development of cervical cancer, HRHPV testing appears to be an alternative. Objective The present study assesses if HRHPV testing can predict CIN2/3 in women referred for mild dyskaryosis and borderline cytological changes in an health authority with a referral policy to colposcopy after one single mild dyskaryotic Pap smear. Study design The HPV DNA Hybrid Capture II (Digene/Abbott, Maidenhead was evaluated on 110 consenting women with mild dyskaryosis and 23 women with persistent borderline changes, who were referred for colposcopy between May and November 2001. A cost comparison between two referral policies was performed. Results CIN2/3 was diagnosed histologically in 30 of 133 women (22% with minor cytological abnormalities. As the Receiver Operator Characteristics plot suggested a cut-off of 3 pg/ml the HRHPV HCII was evaluated at 3 RLU (relative light units and at the manufacturer's recommendation of 1 RLU. At both cut-offs sensitivity and negative predictive value were high at 97%. Specificity was low at 37% at a cut-off of 1 pg/ml and 46% at a cut-off of 3 RLU. To remain cost neutral in comparison to immediate colposcopy the costs for one HR HPV HC II must not exceed £34.37 per test at a cut off of 3 pg/ml. Conclusion The negative likelihood ratio (NLR was of good diagnostic value with 0.089 at 1 RLU and 0.072 at 3 RLU, which reduces the post-test probability for CIN2/3 to 2% in this population. Women with minor cytological disorders can be excluded from colposcopy on a negative HR HPV result. Specificity can be improved by restricting HR HPV testing to women with persistent borderline

  7. Cervical histology after routine ThinPrep or SurePath liquid-based cytology and computer-assisted reading in Denmark

    DEFF Research Database (Denmark)

    Rebolj, Matejka; Rask, Johanne; van Ballegooijen, Marjolein;

    2015-01-01

    BACKGROUND: We compared the sensitivity and specificity of liquid-based cytology (LBC) and computer-assisted reading for SurePath/FocalPoint and ThinPrep with those of manually read conventional cytology in routine cervical screening in four Danish laboratories. METHODS: Using data from five nati...

  8. Comparison of three human papillomavirus DNA assays and one mRNA assay in women with abnormal cytology

    DEFF Research Database (Denmark)

    Rebolj, Matejka; Lynge, Elsebeth; Ejegod, Ditte;

    2014-01-01

    OBJECTIVE: To compare the clinical characteristics of four human papillomavirus (HPV) assays: hybrid capture 2 (HC2), cobas, CLART, and APTIMA in Danish women with abnormal cytology. METHODS: SurePath samples from 367 consecutive women from Copenhagen, with atypical squamous cells of undetermined...

  9. Fine needle aspiration cytology: a tool to diagnose cervical and vaginal endometriosis in low-income places.

    Science.gov (United States)

    Oliveira-Filho, Manoel; Rao, Vietla S; Eleutério, José; Medeiros, Francisco C

    2013-01-01

    Regarded as infrequent, vaginal and cervical endometriosis is probably more common than is generally realized. The apparent rarity of the lesion may be ascribed to the limited awareness of its clinical appearance, combined with technical difficulty in obtaining suitable biopsy material for confirmation. Thus, clinical recognition and tissue confirmation become essential. This paper focuses on vaginal and cervical endometriosis, documenting the clinical, macroscopic, cytological and colposcopic findings in 4 cases seen at a single physical vaginal examination. Diagnosis in these patients was facilitated and improved by fine needle aspiration cytology and confirmed by histology. This technique, which is not used for the diagnosis of endometriosis, could be an option in low-income areas. PMID:23406608

  10. Knowledge, attitudes and practices on cervical cytology-uterine in women from sincelejo and Cartagena, colombia

    Directory of Open Access Journals (Sweden)

    Barrrios-Garcia Lia

    2011-12-01

    Full Text Available Although in Colombia screening programs of cervical cancer have achieved high levelsof coverage, cervical cancer has the first places in incidence and mortality. There isthe need to identify factors influencing it, among them is to investigate the level ofawareness of women about the screening test, their attitudes to screening and itspractices. For this we made a survey of 505 women 13 to 60 years who have had sexual life, living in the cities of Cartagena and Sincelejo (Colombia.The results showthat virtually all women have knowledge of cervical cytology, 94.5% of women havemade at least once. This percentage is higher in the range of 40 to 60 years where itreaches 99% in younger women this percentage decrease. In defining its usefulness,only 73.8% responded accurately. 50% reported unpleasant aspects related to themaking of the cytology, such as fear, and fear of a cancer diagnosis. A fail to rememberis the main factor for not claiming the results.These data show that the percentage ofwomen with sexual life that ignores the usefulness of cytology or never have practicedis minimal, which leads to the conclusion that ineffective screening programs to reducecervical cancer rates depends on other factors that should be investigated.RESUMEN:Aunque en Colombia los programas de prevención del cáncer de cérvix han alcanzadoaltos niveles de cobertura, todavía este ocupa los primeros lugares en incidencia ymortalidad. Existe la necesidad de identificar los factores que influyen en ello, por tantoes importante investigar el nivel de conocimiento de las mujeres sobre la prueba detamizaje, su actitud frente a la misma y sus prácticas relacionadas. Se realizó encuestaa 505 mujeres entre 13 y 60 años edad, que habían tenido vida sexual, residentes en lasciudades de Cartagena y Sincelejo (Colombia. Los resultados revelan que prácticamentetodas las mujeres tienen conocimientos sobre citología cérvico-uterina. El 94.5% delas mujeres se la han

  11. A method for quantitative analysis of clump thickness in cervical cytology slides.

    Science.gov (United States)

    Fan, Yilun; Bradley, Andrew P

    2016-01-01

    Knowledge of the spatial distribution and thickness of cytology specimens is critical to the development of digital slide acquisition techniques that minimise both scan times and image file size. In this paper, we evaluate a novel method to achieve this goal utilising an exhaustive high-resolution scan, an over-complete wavelet transform across multi-focal planes and a clump segmentation of all cellular materials on the slide. The method is demonstrated with a quantitative analysis of ten normal, but difficult to scan Pap stained, Thin-prep, cervical cytology slides. We show that with this method the top and bottom of the specimen can be estimated to an accuracy of 1 μm in 88% and 97% of the fields of view respectively. Overall, cellular material can be over 30 μm thick and the distribution of cells is skewed towards the cover-slip (top of the slide). However, the median clump thickness is 10 μm and only 31% of clumps contain more than three nuclei. Therefore, by finding a focal map of the specimen the number of 1 μm spaced focal planes that are required to be scanned to acquire 95% of the in-focus material can be reduced from 25.4 to 21.4 on average. In addition, we show that by considering the thickness of the specimen, an improved focal map can be produced which further reduces the required number of 1 μm spaced focal planes to 18.6. This has the potential to reduce scan times and raw image data by over 25%. PMID:26477005

  12. A COMPARATIVE STUDY OF FINE NEEDLE ASPIRATION CYTOLOGY AND OPEN BIOPSY IN THE DIAGNOSIS OF CERVICAL LYMPHADENOPATHY IN RURAL TERTIARY CENTRE

    Directory of Open Access Journals (Sweden)

    Karunamoorthy

    2016-04-01

    Full Text Available ABSTRACT Lymphadenopathy is a very common clinical manifestation of many diseases, defined as an abnormality in the size or character of lymph nodes, caused by the invasion or propagation of either inflammatory cells or neoplastic cells into the node. The clinical examination may be inaccurate in differentiating benign from neoplastic enlargement of the lymph node. Surgical excision of a palpable peripheral lymph node is relatively simple; it does require anaesthesia, strict sterility and theatre time and it leaves behind a scar. Fine needle aspiration cytology offers the advantages of an immediate with little cost and trauma. The present study is being undertaken to evaluate its accuracy in the diagnosis of tuberculosis and malignancy of the cervical lymph nodes. AIM AND OBJECTIVE To assess the usefulness of FNAC in the diagnosing cervical lymph node enlargement and cytological features of common pathological conditions affecting the lymph nodes and to evaluate the diagnostic accuracy of FNAC and open biopsy in cervical lymphadenopathy. MATERIALS AND METHODS A total of 100 patients were subjected for the study at CMCH and RC, Irungalur. FNAC was performed under aseptic precaution of the cervical lymph nodes using a 22G needle and HPE correlation was obtained for these patients remaining. These patients were subjected to surgical excision for radical dissection and the HPE correlation for specimen was obtained. RESULTS The result shows that the age wise distribution of patient with benign lesions were within 21-25 age groups and those with malignant lesion were in 31-50 age groups. Among the incidence of male-to-female ratio was 107:93. The duration of symptom was <6 months (<3 cm 50%/3-6 months 45%. The gross morphology shows that TB adenitis patient with positive matting nodes were 18 out of 25 patients were positive for matted nodes (94.47%. According to AJCC cancer staging the size of the nodes were assessed, which shows most of the nodes <3

  13. Cytological detection of cervical carcinoma with new cytochemical markers and flow microanalysis. [Mithramycin, chromomycin

    Energy Technology Data Exchange (ETDEWEB)

    Jensen, R.H.; King, E.B.; Mayall, B.H.

    1976-04-21

    Human cervical cell samples have been stained with mithramycin or chromomycin A3 in an effort to analyze such preparations for premalignant abnormal cells by flow cytometry. Fluorescence from mithramycin or chromomycin A3-stained cells is similar to the fluorescence from DNA in solution when it is complexed with these same antibiotics. Mithramycin or chromomycin A3-stained cells exhibit nuclear specific fluorescence which, for exponentially grown tissue culture cells, reflects the cellular DNA content. All these facts indicate that DNA is the sole intracellular binding site for these antibiotics. Flow cytofluorometry on mithramycin or chromomycin A3-stained cervical cells using a single parameter, fluorescence intensity per cell, appears to be a poor diagnostic procedure. However, simultaneous analysis of cellular fluorescence and small angle light scatter permits a relatively detailed description of each cell sample and appears to be useful for automated sample diagnosis. Qualitative diagnostic analysis based on comparisons of two parameter histograms agrees moderately well with cytomorphological diagnosis on the same cell samples. A technique for quantitation in comparing two parameter histograms is presented and promises to be useful for further progress in flow analysis of human cervical cell samples.

  14. Comparison of conventional Papanicolaou smear and SurePath® liquid-based cytology in the Copenhagen population screening programme for cervical cancer

    DEFF Research Database (Denmark)

    Kirschner, Benny; Simonsen, Kåre; Junge, Jette

    2006-01-01

    OBJECTIVE: To compare diagnostic performance of conventional Papanicolaou smear with SurePath liquid-based cytology in a population screening programme. METHODS: A retrospective comparison was performed on data from two 18-month periods of the screening programme for cervical cancer...... in the municipality of Copenhagen with conventional Papanicolaou technique (n = 82,116) and liquid-based cytology (n = 84,414). RESULTS: After the conversion to liquid-based cytology the percentage of unsatisfactory samples decreased from 2.3% to 0.3% (P cervical samples lacking......-based technique. CONCLUSIONS: This study showed the number of unsatisfactory samples to be significantly reduced with the liquid-based technique. The data suggest that there is an increased detection rate of cervical precancerous lesions with liquid-based cytology, but the number of false positive tests is still...

  15. Clinical utility of Liqui-PREP™ cytology system for primary cervical cancer screening in a large urban hospital setting in China

    Directory of Open Access Journals (Sweden)

    Deshou Hao

    2009-01-01

    Full Text Available Background: Liquid based cytology (LBC has been reported to increase the sensitivity of cervical cytology, in comparison with conventional cytology Pap smear (CPS. Most LBC systems though require expensive automated devices. Aims: To evaluate the efficiency of a new and inexpensive LBC system - LPT cytology system. Materials and Methods: Cervical screening was performed on 31500 patients utilizing the LPT cytology system test from January 2006 to May 2007. A similar number (n = 31500 of CPS were performed from January 2004 to July 2006. All cytology positive patients underwent colposcopy and cervical biopsy with histopathology examination. Fifty cases positive both on cytology and biopsy were submitted to the high-risk human papillomavirus (HPV L1 protein (HR-HPV L1 tests. Results: The LPT cytology system adequately preserved cellular structure for morphologic evaluation. There was a significant difference of the histology/cytology diagnosis concordant rate between that of the CPS and LPT systems [93.6 vs. 78.4%, p=0.001]. The significant higher concordant rate was also seen in the low grade intraepithelial lesion (LSIL (95.4 vs. 78.9%, p=0.001 and in high grade intraepithelial lesion (HSIL (90.2 vs. 76.1%, p=0.001 cytology diagnosis. There was no statistical difference in rate in atypical glandular cells (AGC (61.5 vs. 60% and glandular cell carcinoma (GCC (83.3 vs. 80%. LPT resulted in a marked increased global detection over the CPS. Nuclear expression of HPV L1 was seen in 34% (17/50 of cases. Conclusions: LPT showed an increase in detection rate compared to CPS (P = 0.001 and a significantly higher histological versus cytological concordant referral rate.

  16. Value of Cervical Smear Cytology for Cervical Cancer Screening%宫颈刮片脱落细胞学检查对宫颈癌筛选的价值

    Institute of Scientific and Technical Information of China (English)

    黄瑞虹

    2013-01-01

    Objective:To explore the clinical value of cervical smear cytology for cervical cancer screening.Methods:5963 cases of gynecological examination of women underwent the cervical scraping levy cytology and TCT detection,the positive rate was compared.The TCT test result showed positive or suspected positive underwent colposcopy sampling biopsy,and was compared with the results of TCT test.Results:The positive rate of TCT detection of cervical lesions was significantly higher than that in the control group(P<0.01).There were a total of 538 cases of positive or suspected positive subjects by TCT detected, 449 cases of cervical precancerous lesions detected by biopsy,the detection rate was 83.46%.Conclusion:TCT detection of cervical precancerous lesions can help to improve clinical detection rate,the more significant of TCT diagnosis of abnormalities,the detection rate of biopsy is higher.%  目的:探讨宫颈刮片脱落细胞学检查对宫颈癌筛查的临床价值。方法:5963例妇科体检妇女均行宫颈刮片脱落细胞学检查及TCT检测,比较阳性率;TCT检测结果为阳性或疑似阳性受检者行阴道镜取样病理活检,并与TCT检测结果比较。结果:TCT检测宫颈病变的阳性率明显高于对照组(P<0.01);TCT检测共发现阳性或疑似阳性受检者538例,病理活检共检出宫颈癌前病变449例,检出率83.46%。结论:TCT检测有利于提高宫颈癌前病变的临床检出率,TCT诊断的异常性越显著,病理活检的检出率越高。

  17. Analysis on results of cervical liquid-based cytology in 11030 cases%11030例宫颈液基细胞学检查结果分析

    Institute of Scientific and Technical Information of China (English)

    江海燕

    2011-01-01

    Objective: To explore the value of thin layer cytology test (TLT) plus cervical liquid -based cytology in gyrecological screening. Methods: The results of cervical liquid - based cytology in 11 030 patients from December 2006 to June 2009 were analyzed, all the patients were given TBS reports; 711 positive patients received histological biopsy, the results of cervical liquid -based cytology and histological biopsy were compared. Results: Among 1 1 030 patients receiving cervical liquid -based cytology, 757 patients were diagnosed as above-ASCUS pathological changes, the positive detection rate was 6. 86%. Among 711 positive patients, 165 patients were found with LSIL, 96 patients were diagnosed as condyloma and cervical intraepithelial neoplasia (CIN), the accordance rate was 58. 18%; 43 patients were found with excessive diagnosis ( inflammation), accounting for 26. 06%; 26 patients were found with under - diagnosis (CtN Ⅱ and CIN Ⅲ ) ( 15. 76% ) . Among 49 patients with HSIL, 25 patients were diagnosed as CIN Ⅱ and CIN Ⅲ, the accordance rate was 51.02%; 23 patients were found with excessive diagnosis, accounting for 45. 10% (3 patients with inflammation, 2 patients with condyloma and 18 patients with CIN Ⅰ ), one patient was found with under- diagnosis (squamous cell carcinoma); among 497 patients with ASC -US, ASC -H .and ACC- NOS, 114 patients were diagnosed as condyloma and CIN, the detection rate was 22. 94%. Conclusion: TLT plus cervical liquid -based cytology is an effective screening method of cervical precancerous lesion, but it has false negative and positive results, the cases with abnormal results of cervical liquid - based cytology should be confirmed furtherly by biopsy.%目的:探讨TLT - plus 液基细胞学检测系统在妇科普查中的价值.方法:分析该院2006年12月~2009年6月11 030例宫颈液基细胞学检查结果,所有病例均采用宁波美生医疗器材有限公司的液基细胞学产品(TLT-plus

  18. EUROarray human papillomavirus (HPV) assay is highly concordant with other commercial assays for detection of high-risk HPV genotypes in women with high grade cervical abnormalities.

    Science.gov (United States)

    Cornall, A M; Poljak, M; Garland, S M; Phillips, S; Machalek, D A; Tan, J H; Quinn, M A; Tabrizi, S N

    2016-06-01

    The purpose of this study was to evaluate the performance of the EUROIMMUN EUROArray HPV genotyping assay against the Roche Cobas 4800, Roche HPV Amplicor, Roche Linear Array and Qiagen Hybrid Capture 2 assays in the detection of high-risk HPV (HR-HPV) from liquid based cervical cytology samples collected from women undergoing follow-up for abnormal cervical cytology results. Cervical specimens from 404 women undergoing management of high-grade cytological abnormality were evaluated by EUROarray HPV for detection of HR-HPV genotypes and prediction of histologically-confirmed cervical intraepithelial neoplasia grade 2 or higher (≥CIN2). The results were compared to Hybrid Capture 2, Cobas 4800 HPV, Amplicor and Linear Array HPV. Positivity for 14 HR-HPV types was 80.0 % for EUROarray (95 % CI; 75.7-83.8 %). Agreement (κ, 95 % CI) between the EUROarray and other HPV tests for detection of HR-HPV was good to very good [Hybrid Capture κ = 0.62 (0.54-0.71); Cobas κ = 0.81 (0.74-0.88); Amplicor κ = 0.68 (0.60-0.77); Linear Array κ = 0.77 (0.70-0.85)]. For detection of HR-HPV, agreement with EUROarray was 87.90 % (Hybrid Capture), 93.58 % (Cobas), 92.84 % (Amplicor) and 92.59 % (Linear Array). Detection of HR-HPV was not significantly different between EUROarray and any other test (p < 0.001). EUROarray was concordant with other assays evaluated for detection of high-risk HPV and showed sensitivity and specificity for detection of ≥ CIN2 of 86 % and 71 %, respectively. PMID:27048314

  19. 8781例宫颈液基细胞学分析%Liquid-based cytology test analysis of 8 781 cervical cases

    Institute of Scientific and Technical Information of China (English)

    王红群

    2014-01-01

    Objective To investigate the characteristics of cervical intraepithelial lesions through analyzing the liquid -based cytology test results of 8 781 cases.Methods We used liquid-based cytology test,and the criteria of the Bethesda system (TBS) were adopted. The abnormal cytological examination results of 199 cases were compared with those of histopathological results .Results Four hundred and ten cases(4.7%) had squamous epithelial lesions which were positively correlative with patients ’age(r=0.105,P=0.036). Three cases(0.03%) had glandular epithelium lesions .Liquid-based cytology test had high positive accuracy with histopathological re-sult of biopsy .Conclusions Adult women should be regularly examined by liquid-based cytology test in order to find precancerous le-sions as early as possible and have early treatment .%目的:分析8781例宫颈液基细胞学检查结果及其与组织学检测的相关性。方法采用宫颈液基细胞学检查,细胞学诊断采用TBS进行分析,199例异常细胞学检查与活检组织学进行对比分析。结果410例(4.7%)标准有鳞状上皮病变,3例(0.03%)有腺上皮病变,细胞的鳞状上皮病变与年龄大小呈正相关(r=0.105,P=0.036)。活检与细胞学检查阳性符合率高。结论成年女性应常规做宫颈液基细胞学检查,以便早期发现癌前病变,及时治疗。

  20. Carcinogenic HPV prevalence and age-specific type distribution in 40,382 women with normal cervical cytology, ASCUS/LSIL, HSIL, or cervical cancer

    DEFF Research Database (Denmark)

    Kjær, Susanne K; Munk, Christian; Junge, Jette;

    2014-01-01

    BACKGROUND: Assessment of the prevaccination type-specific prevalence of human papillomavirus (HPV) in the general population is important for the prediction of the impact of HPV vaccination. METHODS: We collected consecutively residual specimens from liquid-based cytology samples from 40,382 women...... % in cervical cancer and the corresponding prevalence of HPV16/18/31/33/45/52/58 was 89 %. CONCLUSION: This study forms a valuable starting point for monitoring the effect of HPV vaccination in Denmark. In addition, the particular carcinogenic role of HPV16 and 18 is confirmed and may support a role...... from the general population in Copenhagen, Denmark, during 2002-2005. All samples were tested for high-risk HPV using the Hybrid Capture 2 technique, and genotyping was done using LiPa (Innogenetics). Through linkage with the Pathology Data Bank, we obtained information on the cytology result...

  1. Promoter Methylation of p16INK4A, hMLH1, and MGMT in Liquid-Based Cervical Cytology Samples Compared with Clinicopathological Findings and HPV Presence

    Directory of Open Access Journals (Sweden)

    Aris Spathis

    2011-01-01

    Full Text Available Cervical cancer is a common cancer inflicting women worldwide. Even though, persistent infection with oncogenic Human Papillomavirus (HPV types is considered the most important risk factor for cervical cancer development, less than 5% of women with HPV will eventually develop cervical cancer supporting that other molecular events, like methylation-dependent inactivation of tumor suppressor genes, may cocontribute in cervical carcinogenesis. We analyzed promoter methylation of three candidate genes (p16, MGMT, and hMLH1 in 403 liquid-based cytology samples. Methylation was commonly identified in both benign and pathologic samples and correlated with higher lesion grade determined by cytological, colposcopical, or histological findings, with HPV DNA and mRNA positivity of specific HPV types and p16INK4A protein expression. Overall accuracy of methylation is much lower than traditional diagnostic tests ranking it as an ancillary technique with more data needed to identify the exact value of methylation status in cervical carcinogenesis.

  2. Comparative analysis of DNA quantitative cytology and TCT in cervical cancer screening%宫颈癌筛查中DNA定量分析与薄层液基细胞学检查对比分析

    Institute of Scientific and Technical Information of China (English)

    钟萍萍; 顾依群; 王军; 周秋立; 王爱春

    2014-01-01

    Objective To compare the cytology diagnostic accuracy of DNA quantitative cytology and thinprep cytology test(TCT) in cervical cancer screening for exploring effective method in cervical cancer screening.Methods TCT and DNA quantitative cytology were carried out in 7 470 women.Women with positive results additionally underwent high risk human papillomavirus (HPV) detection.Positive cytologic diagnosis included atypical squamous cells(ASC) or above in TCT and DNA index 2.5 or above in DNA quantitative cytology.Results The positive rate was 13.0% in method of DNA quantitative cytology and 13.7% in method of TCT in 7 470 cases.Positive rate of the two methods had no significant difference in cervical cancer screening(x2 =1.813,P =0.178).There was significant difference in positive rate of TCT between cases with DNA index≥2.5,<4.5,heteroploid cells more than 3 or DNA index≥4.5 and cases with DNA index≥2.5,<4.5,heteroploid cells less than 3.Every grade of TCT abnormality had abnormal DNA index.Abnormality of DNA index had an increasing trend with the severity of TCT.Infection rate of high risk HPVs had significant difference in different grades of DNA index (x2 =62.648,P =0.000).Conclusion Combination of DNA quantitative cytology and TCT is an effective method in cervical cancer screening,which can reduce misdiagnosis,guide cervical biopsy and suggest infection of high risk of HPVs.%目的 比较DNA定量分析与薄层液基细胞学(TCT)检查在宫颈癌中的应用价值,以提高细胞学诊断的准确性.方法 对7 470例妇女行宫颈薄层液基细胞学(TCT)及DNA定量分析,结果异常者用二代基因杂交捕获法(HC2)检测高危型人乳头状瘤病毒(HPV),TCT在ASC及以上病变为阳性,DNA指数≥2.5为阳性.结果 7470例患者,用DNA定量分析法检测阳性率为13.0%,TCT检查阳性率为13.7%,两种检查方法差异无统计学意义(x2 =1.813,P=0.178);≥3个细胞2.5≤DNA指数<4.5者及DNA指数≥4.5

  3. Cervical neuro-muscular syndrome: discovery of a new disease group caused by abnormalities in the cervical muscles.

    Science.gov (United States)

    Matsui, Takayoshi; Ii, Kunio; Hojo, Shuntaro; Sano, Keiji

    2012-01-01

    Our previous study of whiplash injury found that abnormalities in the cervical muscles cause autonomic dystonia. Further research has found that abnormalities in the cervical muscles cause headache, chronic fatigue syndrome, vertigo, and dizziness. We named this group of diseases cervical neuro-muscular syndrome. Patients treated within a 2-year period from April 1, 2002 to March 31, 2004 reported good outcomes in 83.8% for headache, 88.4% for vertigo and dizziness, 84.5% for chronic fatigue syndrome, 88.0% for autonomic dystonia, and 83.7% for whiplash-associated disorder. A large number of outpatients present with general malaise, including many general physical complaints without identifiable cause. We propose that treatment of the cervical muscle is effective for general malaise.

  4. Histology verification demonstrates that biospectroscopy analysis of cervical cytology identifies underlying disease more accurately than conventional screening: removing the confounder of discordance.

    Directory of Open Access Journals (Sweden)

    Ketan Gajjar

    Full Text Available BACKGROUND: Subjective visual assessment of cervical cytology is flawed, and this can manifest itself by inter- and intra-observer variability resulting ultimately in the degree of discordance in the grading categorisation of samples in screening vs. representative histology. Biospectroscopy methods have been suggested as sensor-based tools that can deliver objective assessments of cytology. However, studies to date have been apparently flawed by a corresponding lack of diagnostic efficiency when samples have previously been classed using cytology screening. This raises the question as to whether categorisation of cervical cytology based on imperfect conventional screening reduces the diagnostic accuracy of biospectroscopy approaches; are these latter methods more accurate and diagnose underlying disease? The purpose of this study was to compare the objective accuracy of infrared (IR spectroscopy of cervical cytology samples using conventional cytology vs. histology-based categorisation. METHODS: Within a typical clinical setting, a total of n = 322 liquid-based cytology samples were collected immediately before biopsy. Of these, it was possible to acquire subsequent histology for n = 154. Cytology samples were categorised according to conventional screening methods and subsequently interrogated employing attenuated total reflection Fourier-transform IR (ATR-FTIR spectroscopy. IR spectra were pre-processed and analysed using linear discriminant analysis. Dunn's test was applied to identify the differences in spectra. Within the diagnostic categories, histology allowed us to determine the comparative efficiency of conventional screening vs. biospectroscopy to correctly identify either true atypia or underlying disease. RESULTS: Conventional cytology-based screening results in poor sensitivity and specificity. IR spectra derived from cervical cytology do not appear to discriminate in a diagnostic fashion when categories were based on

  5. Long-term risk of cervical intraepithelial neoplasia grade 3 or worse according to high-risk human papillomavirus genotype and semi-quantitative viral load among 33,288 women with normal cervical cytology

    DEFF Research Database (Denmark)

    Thomsen, Louise T; Frederiksen, Kirsten; Munk, Christian;

    2015-01-01

    2002-2005, residual liquid-based cervical cytology samples were collected from women screened for cervical cancer in Copenhagen, Denmark. Samples were HPV-tested with Hybrid Capture 2 (HC2) and genotyped with INNO-LiPA. Semi-quantitative viral load was measured by HC2 relative light units in women......HPV genotyping during cervical cancer screening may help identify women at highest risk of CIN3+....

  6. Relation between psychiatric disorder and abnormal illness behaviour in patients undergoing operations for cervical discectomy

    OpenAIRE

    Taylor, R.; Creed, F; Hughes, D.

    1997-01-01

    OBJECTIVE—To test the hypothesis that depression in patients being considered for cervical disc surgery is associated with severe organic pathology. Secondly, to test whether depression and abnormal illness attitudes recorded preoperatively would predict poorer recovery.
METHODS—Seventy four patients with pain and disability from cervical arthrosis were examined during investigations before potential cervical surgery. The prevalence of psychiatric disorder was assessed using...

  7. 妊娠相关宫颈细胞形态学特点及其临床处理%Pregnancy related cervical cytological changes and clinical management

    Institute of Scientific and Technical Information of China (English)

    马莉; 卞美璐; 刘军; 王秀红; 庞春红; 陈颖

    2011-01-01

    Objective To investigate characteristics of cervical cytology and management in pregnant women. Methods From Aug. 2006 to Jan. 2010, 5152 pregnant women who received antenatal and postpartum examination underwent cervical cytological screening by liquid-based cytological test (LCT)in China-Japan Friendship Hospital. The cytological diagnosis was in accordance with the Bethesda system (TBS) 2001 diagnosis and classification system.The abnormal LCT results were followed up at 3 months after postpartum. The diagnosis of high-grade squamous intraepithelial lesions (HSIL) and squamous cell carcinoma (SCC) were based on colposcopic examination and biopsy during pregnant. The diagnosis of atypical glandular cells(AGC) was based on curettage and biopsy at postpartum 6 weeks. The histopathology of biopsy were compared and analyzed. Results ( 1 ) Cervical cytological changes related with pregnancy:among 5152 cases, it was found navicular cells in 3215 cases (62. 40% ), decidual cells in 783 cases ( 15.20% ), reactive glandular cells in 369 cases (7. 16% ), and trophoblastic cells in 55 cases (1.07%). (2) LCT results: among 5152 cases, the normal samples were 4125 cases (80.07%), the inflammatory samples were 542 cases (10.52%), and the samples of abnormal epithelial cells were 485cases (9.41%). Among those abnormal cases, 291 cases (5.65%) were in atypical squamous cells (ASC), 153 cases (2. 97%) were in low-grade squamous intraepithelial lesions (LSIL), 33 cases (0. 64%) were in HSIL, 1 case ( 0. 02% ) were in SCC and 7 cases (0. 14% ) were in AGC. (3)Histological pathology results: all women with HSIL and SCC underwent colposcopic examination and biopsy,it was found 28 cases in cervical intraepithelial neoplasia (CIN) Ⅱ - Ⅲ, 1 cases in adenosquamous carcinoma. 7 women underwent curettage and biopsy at postpartum 6 weeks which were diagnosed by AGC,the histopathological diagnosis was all negative. The concordance rate of cytopathologic and histopathologic diagnosis was

  8. p16 INK4a immunocytochemistry on cell blocks as an adjunct to cervical cytology: Potential reflex testing on specially prepared cell blocks from residual liquid-based cytology specimens

    Directory of Open Access Journals (Sweden)

    Vinod B Shidham

    2011-01-01

    Full Text Available Background: p16 INK4a (p16 is a well-recognized surrogate molecular marker for human papilloma virus (HPV related squamous dysplasia. Our hypothesis is that the invasive interventions and related morbidities could be avoided by objective stratification of positive cytologic interpretations by p16 immunostaining of cell block sections of cytology specimens. Materials and Methods: Nuclear immunoreactivity for p16 was evaluated in cell block sections in 133 adequate cases [20 negative for intraepithelial lesion or malignancy, 28 high-grade squamous intraepithelial lesion (HSIL, 50 low-grade squamous intraepithelial lesion (LSIL, 21 atypical squamous cells, cannot exclude HSIL (ASC-H, and 14 atypical squamous cells of undetermined significance (ASCUS] and analyzed with cervical biopsy results. Results: (a HSIL cytology (28: 21 (75% were p16 positive (11 biopsies available - 92% were positive for cervical intraepithelial neoplasia (CIN 1 and above and 7 (25% were p16 negative (3 biopsies available - all showed only HPV with small atypical parakeratotic cells. (b LSIL cytology (50: 13 (26% cases were p16 positive (12 biopsies available - all were CIN1 or above and 37 (74% were p16 negative (12 biopsies available - all negative for dysplasia. However, 9 (75% of these biopsies showed HPV. (c ASC-H cytology (21: 14 (67% were p16 positive (6 biopsies available - 5 showed CIN 3/Carcinoma in situ/Ca and 1 showed CIN 1 with possibility of under-sampling. Cytomorphologic re-review favored HSIL and 7 (33% were p16 negative (5 biopsies available - 3 negative for dysplasia. Remaining 2 cases - 1 positive for CIN 3 and 1 showed CIN 1 with scant ASC-H cells on cytomorphologic re-review with possibility under-sampling in cytology specimen. (d ASCUS cytology (14: All (100% were p16 negative on cell block sections of cervical cytology specimen. HPV testing performed in last 6 months in 7 cases was positive in 3 (43% cases. Conclusion: p16 immunostaining on cell block

  9. TCT联合DNA定量细胞学检查对宫颈病变临床诊断价值研究%The study of TCT and DNA quantitive cytology in the diagnosis of cervical lesions.

    Institute of Scientific and Technical Information of China (English)

    刘桂依; 黄艳美

    2011-01-01

    目的 评价宫颈细胞学配合DNA定量细胞学检查对子宫颈病变的诊断价值.方法 2006年2月至2007年12月间在我们中心对2 800例患者行液基薄层细胞学检查(TCT)及DNA定量细胞学检查.TCT或DNA定量细胞学检查异常者,3个月后复查.TCT未明确诊断意义的不典型鳞状上皮细胞(ASCUS)异常者行阴道镜下多点活组织病理检查.结果 TCT异常者302例,占10.79%(302/2 800).ASCUS 194例,占6.93%;低度鳞状上皮内病变(LSIL)34例,占1.21%;高度鳞状上皮(HSIL)内病变18例,占0.64%(18/2 800);鳞癌(SCC)3例,占0.10%.经阴道镜下多点活组织病理检查,诊断符合率较高.结论 采用TCT配合细胞DNA定量分析,因取材方便无创伤,是进行阴道镜下活检前筛查宫颈癌和癌前病变的好方法,可以帮助早发现宫颈癌和宫颈病变.%Objective To evaluate the diagnostic value of cervical cytology combined with DNA quantitive cytology for cervical lesions.Methods From February 2006 to December 2007, 2,800 patients were examined with liquid - based ThinPrep cytologic test ( TCT ) and DNA quantitative cytology. The patients with abnormal TCT or DNA quantitative cytology results were re - examined after three months. The patients with abnormal TCT results and atypical squamous cells of uncertain significance ( ASCUS ) were further examined by colposcopy and multi - site biopsy examinations. Results Abnormal TCT results were found in 302 patients ( 10.79% ), ASCUS, low - grade squamous intraepithelial lesions ( LSIL ), high - grade squamous intraepithelial lesion ( HSIL ) and squamous cell carcinoma ( SCC ) were found in 194 ( 6.93% ), 34 ( 1.21% ),18 ( 0.64% ) and 3 patients ( 0. 10% ), respectively. Pathological examination confirmed inflammation in 198 cases, accounting for 65.56%( 198/302 ) of abnormal TCT. The difference in TCT and human papillomavirus ( HPV ) infection diagnosis was significant ( P < 0.01 ). Conclusion The combined examinations of

  10. The vaginal stump cytology role in follow-up after treatment of cervical cancer%阴道残端超薄细胞学检查在宫颈癌治疗后随访中的作用

    Institute of Scientific and Technical Information of China (English)

    刘春红; 殷超

    2015-01-01

    Objective To assess the clinical role of vaginal stump cytology in follow-up of cervical cancer patients who had surgery treatment. Method A retrospective analysis of these patients underwent cervical cancer surgery in our hospital between January 1, 2002 and December 31, 2005, and the data of the vaginal stump ultra-thin cytology about these patients were intact during postoperative follow-up. Result Totally, 1244 cases of cervical vaginal exam-inations from 300 female patients were involved in our research. Abnormalities in the vaginal cytology stump exami-nation was showed by 187 cases from 93 patients, which including 83 cases of atypical squamous cells without defi-nitely diagnostic significance, 58 cases of low-grade squamous intraepithelial lesions, 34 cases of highly squamous intraepithelial lesion , 6 cases of atypical glandular cell lesions,6 patients of tumor tendency. 25 patients had CINⅡ or worse lesions were showed in colposcopy examination of these abnormal cases. No recurrence or CINⅢof col-poscopy biopsy was not found in these patients which was also better vaginal stump cytological results than HSIL. Conclusion During follow-up, 1/3 cervical cancer survivors may have abnormal cytology; cytological results for HSIL patients should accept the colposcopy. If there is no visible lesions, ASC-US or LSIL patients cannot to col-poscopy in follow up, unless the abnormalities persist.%目的:评估阴道残端细胞学在宫颈癌随访中的作用。方法回顾分析2002年1月至2005年12月在本院因宫颈癌行手术治疗的患者,对300例患者的1244个阴道残端细胞学结果进行分析。结果93例患者的187个细胞学结果异常,包括83个诊断意义尚不明确的非典型鳞状上皮改变( atypical squamous cell of undetermined significance,ASC-US),58个低度鳞状上皮内病变(low grade squamous intraepithelial le-sion,LSIL),34个高度鳞状上皮内病变( high grade squamous intraepithelial lesion,HSIL),6

  11. Comparison of conventional Papanicolaou smear and SurePath® liquid-based cytology in the Copenhagen population screening programme for cervical cancer

    DEFF Research Database (Denmark)

    Kirschner, Benny; Simonsen, Kåre; Junge, Jette

    2006-01-01

    OBJECTIVE: To compare diagnostic performance of conventional Papanicolaou smear with SurePath liquid-based cytology in a population screening programme. METHODS: A retrospective comparison was performed on data from two 18-month periods of the screening programme for cervical cancer...

  12. Liquid-based cervical cytology using ThinPrep technology: weighing the pros and cons in a cost-effectiveness analysis.

    NARCIS (Netherlands)

    Bekker-Grob, E.W. de; Kok, I.M. de; Bulten, J.; Rosmalen, J. van; Vedder, J.E.M.; Arbyn, M.; Klinkhamer, P.J.; Siebers, A.G.; Ballegooijen, M. van

    2012-01-01

    PURPOSE: Cervical cancer screening with liquid-based cytology (LBC) has been developed as an alternative to the conventional Papanicolaou (CP) smear. Cost-effectiveness is one of the issues when evaluating LBC. Based on the results of a Dutch randomised controlled trial, we conducted cost-effectiven

  13. 宫颈脱落细胞中的DNA甲基化研究%Study of DNA Methylation in Cervical Cytology

    Institute of Scientific and Technical Information of China (English)

    仲肇基

    2012-01-01

    在宫颈病变发生、发展的过程中,DNA甲基化水平发生一系列改变.以往的研究多是基于宫颈组织的,而近来检测宫颈脱落细胞甲基化的研究越来越多.与宫颈组织相比,用宫颈脱落细胞作为研究对象进行检测安全无创,更方便临床应用,可能成为筛查、诊断宫颈病变和判断宫颈病变预后的有利工具.讨论研究较多的宫颈脱落细胞中基因的甲基化水平与宫颈病变等级的关系及其临床应用.%Aberrant promoter methylation has been detected in pathogenesis of many cancers,including cervical cancer. During cervical carcinogenesis,there is a series of alteration in the methylation status of host genes. Recently,the role of promoter hypermethylation as a diagnostic tool for cervical cancer screening has been examined in liquid-based cytology samples. Detection of DNA methylation in exfoliated cervical cytology samples is more convenient and minimally invasive than in biopsy tissues. In those samples .researchers are searching for DNA methylation markers selectively indicating high-grade precancerous lesions, in order to increase the accuracy of cervical cytological diagnosis. The methylation markers might be used as a potential biomarker for cancer screening,diagnosis and predicting clinical outcomes. Here,we discuss methylation status of host genes in foliated cervical cytology samples during cervical carcinogenesis and its clinical impact.

  14. Cytological diagnosis of tuberculous cervicitis: A case report with review of literature

    OpenAIRE

    Kalyani, R; S R Sheela; M Rajini

    2012-01-01

    Tuberculosis of cervix is a rare disease. Tuberculosis usually affects women of childbearing age, indicating hormone dependence of infection. The patient presents with menstrual irregularities, infertility or vaginal discharge. Cervical lesions presents as papillary/vegetative growth or ulceration mimicking cervical cancer. Cervical Papanicolaou (Pap) smear plays an important role in diagnosing the disease by non-invasive technique in which the presence of epithelioid cells and Langhan′s type...

  15. Clinical significance of Thinprep cytology test in screening of cervical lesions%液基薄层细胞学在筛查宫颈病变中的临床意义

    Institute of Scientific and Technical Information of China (English)

    张江菊; 黄寅虎; 郭建新; 李力; 郑秀惠

    2013-01-01

    Objective:To explore the clinical significance of Thinprep cytology test (TCT) in screening of cervical lesions.Methods:TCT screening was conducted among 18 904 patients from outpatient department of Daping Hospital Affiliated to the Third Military Medical University from January to December in 2012,and 688 patients were found with cytological abnormalities (diagnosis of TBS system ≥ ASC-US),then 416 patients underwent colposcopy and biopsy ; the clinical significance of TCT in screening of cervical lesions was evaluated with pathological examination as gold standard.Results:A total of 416 patients were found with cytological abnormalities,including 195 patients with ASC,65 patients with LSIL,138 patients with HSIL,and 52 patients with cervical cancer; 128 patients with cervicitis,106 patients with cervical intraepithelial neoplasia (CIN) Ⅰ,78 patients with CIN Ⅱ,52 patients with CIN Ⅲ,and 52 patients with cervical invasive carcinoma (including one patient with cervical adenocarcinoma) were found by pathological examination; the coincidence rates of diagnosis between pathological examination and TCT were 48.21% (ASC),81.54% (LSIL),89.13% (HSIL),and 100.00% (cervical cancer),respectively; the sensitivity and specificity of TCT for cervical lesions (≥ CIN Ⅱ) were 90.66% and 76.07%,respectively.Conclusion:TCT can improve the detection rate of cervical lesions and it has a high concordance with the histopathological results with high sensitivity,TCT is an effective method for screening cervical lesions and missed diagnosis can be reduced.%目的:探讨液基薄层细胞学在筛查子宫颈病变中的临床意义.方法:对2012年1月~12月第三军医大学大坪医院18 904例门诊患者进行TCT筛查,发现细胞学异常(TBS系统诊断≥ASC-US)的患者688例,其中416例进行了阴道镜检查并取活检,以病理学为标准,评价TCT筛查子宫颈病变的临床意义.结果:细胞学异常的416例患者中,ASC 195例,LSIL65

  16. 18356例宫颈液基细胞学与活检组织病理学结果分析%Analysis the Results of 18 356 Cases of Liquid-based Cervical Cytology and the Histopathology of Colposcopic Biopsy in Cervical Screening/

    Institute of Scientific and Technical Information of China (English)

    江淑萍; 孔友明; 陈冰

    2013-01-01

      目的:探讨宫颈液基细胞学与活检组织病理学在宫颈病变筛查中的价值.方法:对18356例经宫颈液基细胞学检查发现异常者在阴道镜下活检的患者临床及病理资料进行回顾性分析.结果:LCT 显示:18356例中,ASCUS 以上的有1256例,阳性率为6.8%,其中 ASCUS 占51.8%,LSIL占29.5%,HSIL 占10.6%.宫颈活检:慢性炎症占39.7%,CIN Ⅰ占37.3%,CIN Ⅱ占13.6%,CIN Ⅲ占8.9%,宫颈癌占0.4%.结论:LCT 液基细胞学检测系统是一种有效的宫颈癌前病变筛查方法.对 LCT 阳性病例行阴道镜下活检,能大大提高宫颈癌前病变及宫颈癌早期检出率,减少漏诊的发生.%Objective:To evaluate the value of liquid-based cytology (LCT) and histopathology of colposcopic biopsy in cervical screening.Methods:The cervical LCT results of 18 356 cases were analyzed,follow up diagnoses of colposcopic biopsy were available for those cytological abnormalities,then we performed a retrospective analysis of those clinical and pathological data.Results:Among 18 356 patients receiving LCT,1256 patients were diagnosed as Above-ASCUS pathological changes,the positive detection rate was 6.8%.Among these,ASCUS,LSIL and HSIL were 51.8%,29.5% and 10.6%,respectively. Among 1256 patients receiving colposcopic biopsy,39.7% were diagnosed as chronic inflammation,and cervical intraepithelial neoplasiaⅠ(CINⅠ) were 37.3%, CIN Ⅱ were 13.6%,CIN Ⅲ were 8.9% and cervical cancer were 0.4%.Conclusion:LCT liquid-based cytology technology is an effective method in screening of cervical precancerous disease.Combining with colposcopic biopsy among patients with abnormal LCT results,can not only improve the detection rate of cervical precancerous lesions and early stage of cervical cancer,but also reduce the incidence of missed diagnosis,significantly.

  17. Prevalence of High-Risk Human Papillomavirus (HR-HPV) Genotypes and Multiple Infections in Cervical Abnormalities from Northern Xinjiang, China

    Science.gov (United States)

    Du, Jingyun; Jiang, Jianjun; Jia, Xuesong; Chen, Chuangfu; Wang, Yuanzhi

    2016-01-01

    Multiple human papillomavirus (HPV) genotypes often coexist within the cervical epithelia and are frequently detected together in various grades of the cervical neoplasia. To date, only a few reports exist on multiple HPV infections of HPV in Xinjiang Uygur Autonomous Region (XUAR). In the present study, we investigated the prevalence of High-Risk HPV (HR-HPV) genotypes and multiple infections. Cervical cytology samples were collected from 428 women who presented cervical abnormalities. Genotyping of HPV was performed by polymerase chain reaction–sequencing based typing (PCR-SBT) using consensus primers and specific primers. Of them, 166 samples were positive for HPV according to PCR results using the consensus primers. These samples contained cervical abnormalities enriched with inflammation (n = 107), cervical intraepithelial neoplasia (CIN) I (n = 19), CINII-III (n = 9) and cervical cancer (n = 31). Of the 166 HPV positive samples as determined by PCR analysis, 151 were further typed by PCR-SBT using 19 pairs of genotype-specific primers. Using this method, 17 different HR-HPV genotypes were identified. The most frequently observed HPV genotypes were HPV16 (44.0%, 73/166), 53 (28.9%, 48/166), 52 (25.3%, 42/166), 58 (22.3%, 37/166) and 35 (17.5%, 29/166). The proportions of single and multiple infections in the HPV-positive specimens were 34.9% and 65.1%, respectively. Multiple HPV types were most prevalent in the inflammatory state (63.0%), followed by cervical cancer (24.1%), CINI (11.1%), and CINII-III (1.9%). The results of our data analyses suggested that i) multiple HPV infection is not necessarily correlated with the severity of cervical abnormalities; and ii) among the multiple HPV infections, double infections combined with HPV16 is the most common. In addition, L1 full-length sequences of the top five high-risk HPV genotypes were amplified and sequenced. According to the L1 sequence of the epidemic genotypes that were amplified, we found that these

  18. TCT联合HPV检测在宫颈病变筛查中的意义%Cervical Liquid-Based Cytology Combined Human Papillomavirus Testing in Cervical Lesion

    Institute of Scientific and Technical Information of China (English)

    戴一菲; 田荣华

    2012-01-01

    Objective To study the significance of combination of cervical liquid-based cytology and human papillomavirus detection in cervical lesion screening. Methods From March 2010 to December 2011,3,028 patients were detected with nipple shaped tumor virus ( HPV) and cervical liquid base cytology (TCT) ,TCT was used by 2001 International Cancer Association TBS diagnosis system report way,to the patients with positive results of TCT check for ASCIIS and the above lesions and (or) HPV detection, cervical organization biopsy under electronic vaginal mirror were performed. Results The sensitivity of TCT check was 56.72% ,with the specificity of 28. 19% ;the sensitivity of HPV check was 86.03% ,with specific for 46.38% ;among 408 cases of CIN and cervical cancer pathology ,28 cases were single TCT positive (6. 86% ) ;with TCT and HPV positive 203 ca-ses(49. 75% ) ,there was significant difference in diagnosis of cervical squamous intraepithelial neoplasm ( CIN) and cervical cancer(x2 = 184.926,P=0.000) between single TCT check and combination of TCT and HPV examination. Conclusion TCT combined with HPV check can improve the detection rate of cervical cancer and precancerous lesions, improve the sensitivity and specificity of screening,effectively reduce the TCT in detection of false-negative rates,and avoid excessive colposcopy and biopsy in low-risk populations and over-treatment.%目的 探讨宫颈液基细胞学检查与人乳头状瘤病毒检测联合应用在宫颈病变筛查中的意义.方法 选取2010年3月-2011年12月孝感市中心医院病理科同时进行了宫颈液基细胞学(TCT)检查及人乳头状瘤病毒(HPV)检测的患者3028人,TCT采用2001年国际癌症协会TBS诊断系统报告方式,对TCT检查结果为ASCUS及以上病变和(或)HPV检测结果为阳性的患者在电子阴道镜下取宫颈组织活检.结果 TCT检查敏感度为56.72%,特异度为28.19%;HPV检查敏感度为86.03%,特异度为46.38%;病

  19. First trial of cervical cytology in healthy women of urban Laos using by self-sampling instrument.

    Science.gov (United States)

    Nabandith, Viengvansay; Pholsena, Vatsana; Mounthisone, Phouthasone; Shimoe, Kyoko; Kato, Saiko; Aoki, Kunio; Noda, Sadamu; Takamatsu, Reika; Saio, Masanao; Yoshimi, Naoki

    2012-01-01

    Cervical cancer is the most common cancer in Laos women and a screening programme, even with the PAP smear test (PAP test), has yet to be established for routine use. The Pap test is accepted as the most appropriate for cervical cancer screening in some settings but it is not commonly available in Laos hospitals, because there are few cytopathologists and gynecologists have little experience. As a pilot program, seminars for the PAP test were given in 2007 and 2008, and then PAP tests were carried out using self-sampling instrument (Kato's device) with 200 healthy volunteers in Setthathirath hospital, Laos, in 2008. The actual examination number was 196, divided into class I 104 (53.1%), class II 85 (43.3%), class IIIa 4 (2.0%), class IIIb 1 (0.5%), and class V 1 (0.5%) by modified Papanicolau classification. Four cases had menstruation. There were 6 cases with epithelial cell abnormalities including malignancy. There were 7 cases with fungus and 2 cases with trichomonas in Class II. More than 70% volunteers felt comfortable with the Kato's device and wanted to use it next time, because of the avoidance of the embarrassment and a low cost as compared with pelvic examination by gynecologists. This first trial for PAP test for healthy Laos women related to a hospital found three percent to have abnormal cervical epithelial cells. Therefore, this appraoch using a self-sampling device suggests that it should be planned for cervical cancer prevention in Laos.

  20. 宫颈刮片脱落细胞学检查在筛选宫颈癌中的应用分析%Application Analysis the Cervical Scraping Cytology in Screening of Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    张鸥

    2016-01-01

    目的:对宫颈刮片脱落细胞学检查在筛选宫颈癌中的应用进行观察分析。方法选取我院2014年1月~2015年12月收治的214例进行宫颈癌筛查患者作为研究对象,进行宫颈刮片脱落细胞学检查,将宫颈刮片脱落细胞学检查结果同宫颈活组织病理检查相比较,观察分析宫颈刮片脱落细胞学检查在诊断宫颈癌、癌前病变的敏感度、特异度、阴性预测值和阳性预测值等。结果宫颈刮片脱落细胞学检查诊断宫颈癌、癌前病变敏感度为90.43%,特异度为89.17%,阳性预测值为86.73%,阴性预测值为92.24%,诊断准确率为89.72%。结论宫颈刮片脱落细胞学检查在筛查宫颈癌方面具有较高的敏感度。%Objective To observe and analyze the application of cervical scraping cytology in screening of cervical cancer.Methods Selected 214 cases of patients with cervical cancer screening from January 2014 to December 2015 in our hospital, for example, cervical scraping cytology, cervical scraping cytology results with cervical biopsy compared toobserve and analyze the cervical scraping off cytology in the diagnosis of cervical cancer and precancerous lesions of sensitivity, speciifcity, negative predictive and positive predictive value.Results The sensitivity of cervical cancer and precancerous lesion was 90.43%, the speciifcity was 89.17%, the positive predictive value was 86.73%, the negative predictive value was 92.24%, the diagnostic accuracy rate was 89.72%.Conclusion Cervical scraping cytology test in the screening of cervical cancer has a high sensitivity.

  1. Integrated cervical smear screening using liquid based cytology and bioimpedance analysis

    Directory of Open Access Journals (Sweden)

    Lopamudra Das

    2014-01-01

    Conclusion: The integration of bioimpedance observation along with the proposed low-cost monolayer technology could increase the efficiency of the cervical screening to a greater extent thereby reducing the rates of faulty diagnosis.

  2. Effect of intrauterine copper device on cervical cytology and its comparison with other contraceptive methods

    Directory of Open Access Journals (Sweden)

    Sipra Bagchi

    2016-08-01

    Conclusions: There was no significant risk of cervical dysplasia or invasive carcinoma in IUCD users up to 2 years of use while other contraceptives (except barrier one showed increased incidence of mild dysplasia (LSIL after 1 year of use. Though risk of cervical malignancy is less with intrauterine copper devices, regular follow up should be done in long term users. [Int J Reprod Contracept Obstet Gynecol 2016; 5(8.000: 2795-2798

  3. 宫颈细胞DNA倍体定量分析联合液基细胞学在宫颈癌早期筛查中的应用价值%Value of quantitative analysis is of DNA ploidy and cervical liquid-based cytology in the screening of ;cervical cancer and cervical intraepithelial neoplasm

    Institute of Scientific and Technical Information of China (English)

    宋志琴; 王蔼明

    2014-01-01

    Objective To evaluate the application of DNA ploidy detection and cervical liquid-based cytology in the screen methods of cervical cancer and cervical precancerous lesion. Methods 12 630 women were detected by DNA ploidy and cervical liquid-based cytology, and in which of 1 146 cases with DNA aneuploidy and (or) abnormal cervical liquid-based cytology were given cervical biopsy. Pathological diagnosis was taken as gold standard. The clinical significance of two methods in the screen of cervical lesion was analyzed. Results The positive rates of DNA ploidy was significantly higher than cervical liquid-based cytology. Cases which were observed that LSIL and all above decided by cervical liquid-based cytology, and cases with DNA heteroploid cell more than 3 were sent to fixed point biopsy, the sensitivity of 73.28%and the specificity of 76.50%were cervical liquid-based cytology, while the sensitivity of 89.14%and the specificity of 75.80%by quantitative DNA. Conclusion DNA imaging cytometry is better for screening cervical dysplasia and carcinoma of the uterine cervix than conventional cytology.%目的:探讨宫颈细胞DNA倍体检测联合液基细胞学在宫颈癌早期筛查中的应用价值。方法对12630例患者采用宫颈细胞DNA倍体定量检测与液基细胞学联合进行宫颈病变的早期筛查,对其中1146例宫颈细胞DNA倍体定量检测阳性和(或)宫颈液基细胞学阳性者行阴道镜检查并取活检,以病理诊断为金标准,评价两种方法在宫颈癌早期筛查中的作用与意义。结果(1)宫颈DNA异倍体细胞的检出率和宫颈液基细胞学的阳性率分别为13.68%和8.97%,有统计学差异(P<0.01);(2)随着DNA异倍体细胞数量的增加,液基细胞学的阳性率也相应增加,且宫颈病变的严重程度也增加,两种方法检测结果均阳性者与活检病理诊断有较高的符合率;(3)以1~2个倍体异常细胞为宫颈活检标准,发现

  4. Cytological diagnosis of tuberculous cervicitis: A case report with review of literature

    Directory of Open Access Journals (Sweden)

    R Kalyani

    2012-01-01

    Full Text Available Tuberculosis of cervix is a rare disease. Tuberculosis usually affects women of childbearing age, indicating hormone dependence of infection. The patient presents with menstrual irregularities, infertility or vaginal discharge. Cervical lesions presents as papillary/vegetative growth or ulceration mimicking cervical cancer. Cervical Papanicolaou (Pap smear plays an important role in diagnosing the disease by non-invasive technique in which the presence of epithelioid cells and Langhan′s type of giant cells is diagnostic. However, other causes of granulomatous cervicitis should be considered and ruled out. Ziehl-Neelsen (ZN stain for acid fast bacilli, fluorescent technique, biopsy and culture help in confirming the disease. We present the case of a 45-year-old female, who presented with vaginal discharge, dysfunctional uterine bleeding, first degree uterine descent with grade II cystocele and rectocele and cervical ulcer. Pap smear revealed epithelioid cells and Langhan′s type of giant cells, confirmed by ZN stain of cervical smear, fluorescent technique and culture.

  5. Human Papillomavirus Research on the Prevention, Diagnosis, and Prognosis of Cervical Cancer in Taiwan

    OpenAIRE

    Chyong-Huey Lai; Angel Chao; Huei-Jean Huang

    2012-01-01

    Cervical cancer is third in incidence and fourth in mortality among cancers of women worldwide. Epidemiological studies have shown that human papillomavirus (HPV) is necessary, if not sufficient, to cause nearly 100% of cervical cancers. HPV testing is useful in primary screening for cervical neoplasms. The value of HPV detection or genotyping is potentially useful in triage of borderline or low-grade abnormal cervical cytology, follow-up after treatment of cervical intraepithelial neoplasia,...

  6. 宫颈薄层液基细胞学检查联合高危型HPV检测在宫颈病变筛查中的临床应用%CLINICAL APPLICATION OF CERVICAL THIN PREP CYTOLOGIC-TEST AND HIGH-RISK HPV TYPE TESTING IN SCREENING OF CERVICAL LESIONS

    Institute of Scientific and Technical Information of China (English)

    朱华洁

    2012-01-01

    目的 研究宫颈薄层液基细胞学检查(thin prep cytologic test,TCT)联合高危型人乳头瘤病毒(human papilloma virus,HPV)检测在子宫颈病变筛查中的诊断价值.方法对2010年1-12月行宫颈病变筛查的患者680例,进行TCT和高危型HPV检测,同时行阴道镜下组织活检.结果 680例患者中宫颈TCT检查结果异常180例,对宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)诊断的敏感性为85.57%,特异性为97.12%,漏诊率为4.12%.TCT与HPV联合检查,诊断敏感性为89.69%,特异性为85.80%,漏诊率1.62%.HPV感染率为26.91%,随着细胞学和组织学诊断级别的升高,HPV感染率不断上升.结论 TCT与高危型HPV联合检测是筛查宫颈癌及癌前病变的有效方法,可防止漏诊,有利于提高宫颈上皮内瘤变的诊断率.%Objective To explore the diagnostic value of cervical thin prep cytologic test ( TCT ) combined with high - risk human papilloma virus ( HPV ) detection in the screening of cervical lesions. Methods A total of 680 patients for cervical lesions screeing underwent TCT and high - risk HPV type detection in gynecology outpatient from January 2010 to December 2010, meanwhile, biopsy was performed under colposcopy. Results Among 680 cases,the result of cervical TCT was abnormal in 180 cases. The sensitivity, specificity and the rate of missed diagnosis of TCT for the diagnosis of cervical intraepithelial neoplasia ( CIN ) was 85.57% , 97. 12% and 4. 12% , respectively, while thoses of combination of TCT and HPV was 89. 69% ,85. 80% and 1. 62%. The infection rate of HPV was 26. 91%. With the increase of cytological and histological grading, the infection rate of HPV increased. Conclusion TCT combined with high risk HPV detection is an effective method in screening cervical cancer and precancerous lesions. It helps to prevent missed diagnosis and improve the diagnosis rate of CIN.

  7. Evaluation of fine needle aspiration cytology of cervical lymphadenopathy in Bir Hospital

    Directory of Open Access Journals (Sweden)

    Subash Khadka

    2015-09-01

    Full Text Available Background & Objectives: To correlate FNAC of cervical lymphadenopathy with the histopathology.Materials and Methods: Prospective study on 67 patients with cervical lymphadenopathy at the ENT Department, Bir Hospital, from January 2013 to July 2014. Patients were subjected to both FNAC and histopathology.Results: Thirty cases (44.8% were females and 37 cases (55.2% were males. Tuberculosis was most common disease in 30(44.80% patients. Sensitivity, specificity, and diagnostic accuracy of FNAC to diagnose tubercular lymphadenopathies were 83.0%, 100.0%, and 92.54% respectively. Overall correlation of FNAC to histopathology was 86.57%.Conclusion: FNAC is very simple and accurate technique for diagnosis of cervical lymphadenopathy.JCMS Nepal. 2015;11(1: 22-25

  8. Diagnostic value of cervical cytological examination, HPV-DNA test combined with colposcopy examination in cervical lesions diagnosis%宫颈细胞学检查、HPV-DNA检测配合阴道镜检查对子宫颈病变的诊断价值

    Institute of Scientific and Technical Information of China (English)

    李玉欢

    2013-01-01

    目的 分析与探讨宫颈细胞学检查、HPV-DNA(人乳头瘤病毒基因检测)检测配合阴道镜检查对于子宫颈病变的诊断价值.方法 选取本院2010年8月至2012年8月期间收治的接受子宫颈病变筛查的妇女共1020例,对其实施宫颈细胞学检查和HPV-DNA配合阴道镜检查,检查结果与组织学金标准进行比对,以对比两种方法的准确性与诊断价值.结果 采取HPV-DNA配合阴道镜检查为异常结果的检出率较宫颈细胞学检查更高,差异具有统计学意义(P<0.05),前者检出异常共64例,后者检出异常共34例.结论 宫颈细胞学检查、HPV-DNA检测以及阴道镜检查对于子宫颈病变均有一定的诊断价值,而采用HPV-DNA检测配合阴道镜检查能够帮助医生较早地发现患者的宫颈癌前病变,为患者的治疗方案的选择提供科学依据.%Objective To analyze and discuss the diagnostic value of cervical cytological examination,HPV-DNA test combined with colposcopy examination in diagnosis of cervical lesions.Methods From August 2010 to August 2012 in our hospital a total of 1020 cases of women who underwent cervical diseases screening,were implemented with cervical cytological examination and HPV-DNA combined with colposcopy examination,the correlated pathological results were compared with the gold standard in accuracy and diagnostic value of two methods.Results The HPV-DNA abnormal results detection rate was higher than that of cervical cytological examination combined with colposcopy examination,with statistically significant difference,former found 64 abnormal cases,the latter examination found 34 cases of abnormal.Conclusion Cervical cytological examination,HPV-DNA testing combined with colposcopy examination for cervical lesions has certain diagnostic value,and the combined use of HPV-DNA testing and colposcopy examination could help doctors find early precancerous lesion of cervical cancer,and provide scientific basis for the

  9. Tele-cytology: An innovative approach for cervical cancer screening in resource-poor settings.

    Science.gov (United States)

    Singh, Sandeep; Badaya, Sorabh

    2016-01-01

    Carcinoma cervix remains a leading cause of cancer mortality among women in countries lacking any screening program. The existing screening policy and approach via conventional cytology centered mainly in Tertiary Care Center, is totally unaffordable to Indian women, especially in the remote areas. This suggests the need of depolarizing the resources via generating the near real time modalities which could be used at the door step of the needy ones. For any screening modality to be effective it should be adequately sensitive, specific, reproducible, cheap, simple, affordable, and the most important is should be real time to ensure wide coverage and curtail loss to follow-up. Incorporating telecytology as a screening tool could make the dream come true. Telecytology is the interpretation of cytology material at a distance using digital images. Use of mobile telecytology unit housed in a van carrying satellite equipment and the automated image capturing systems is the central theme behind this idea. The imaging equipment would be carrying out the imaging of Papanicolaou smears prepared at the screening site and sending the images to the central laboratories situated at some tertiary care level. This concept could overcome the hindrance of trained cytology infrastructure in the resource poor settings and could provide an efficient and economical way of screening patients. There is possibility that the designed approach may not detect the entire women positive for the disease but if the desired objective was to diagnose as many cases as possible in resource poor setting, then this process offers an advantage over no screening at all. PMID:27461597

  10. Tele-cytology: An innovative approach for cervical cancer screening in resource-poor settings

    Directory of Open Access Journals (Sweden)

    Sandeep Singh

    2016-01-01

    Full Text Available Carcinoma cervix remains a leading cause of cancer mortality among women in countries lacking any screening program. The existing screening policy and approach via conventional cytology centered mainly in Tertiary Care Center, is totally unaffordable to Indian women, especially in the remote areas. This suggests the need of depolarizing the resources via generating the near real time modalities which could be used at the door step of the needy ones. For any screening modality to be effective it should be adequately sensitive, specific, reproducible, cheap, simple, affordable, and the most important is should be real time to ensure wide coverage and curtail loss to follow-up. Incorporating telecytology as a screening tool could make the dream come true. Telecytology is the interpretation of cytology material at a distance using digital images. Use of mobile telecytology unit housed in a van carrying satellite equipment and the automated image capturing systems is the central theme behind this idea. The imaging equipment would be carrying out the imaging of Papanicolaou smears prepared at the screening site and sending the images to the central laboratories situated at some tertiary care level. This concept could overcome the hindrance of trained cytology infrastructure in the resource poor settings and could provide an efficient and economical way of screening patients. There is possibility that the designed approach may not detect the entire women positive for the disease but if the desired objective was to diagnose as many cases as possible in resource poor setting, then this process offers an advantage over no screening at all.

  11. FTIR Microspectroscopy Coupled with Two-Class Discrimination Segregates Markers Responsible for Inter- and Intra-Category Variance in Exfoliative Cervical Cytology

    Directory of Open Access Journals (Sweden)

    Mark A. Pitt

    2008-01-01

    Full Text Available Infrared (IR absorbance of cellular biomolecules generates a vibrational spectrum, which can be exploited as a “biochemical fingerprint” of a particular cell type. Biomolecules absorb in the mid-IR (2–20 μm and Fourier-transform infrared (FTIR microspectroscopy applied to discriminate different cell types (exfoliative cervical cytology collected into buffered fixative solution was evaluated. This consisted of cervical cytology free of atypia (i.e. normal; n = 60, specimens categorised as containing low-grade changes (i.e. CIN1 or LSIL; n = 60 and a further cohort designated as high-grade (CIN2/3 or HSIL; n = 60. IR spectral analysis was coupled with principal component analysis (PCA, with or without subsequent linear discriminant analysis (LDA, to determine if normal versus low-grade versus high-grade exfoliative cytology could be segregated. With increasing severity of atypia, decreases in absorbance intensity were observable throughout the 1,500 cm−1 to 1,100 cm−1 spectral region; this included proteins (1,460 cm−1, glycoproteins (1,380 cm−1, amide III (1,260 cm−1, asymmetric (νas PO2 − (1,225 cm−1 and carbohydrates (1,155 cm−1. In contrast, symmetric (νs PO2 − (1,080 cm−1 appeared to have an elevated intensity in high-grade cytology. Inter-category variance was associated with protein and DNA conformational changes whereas glycogen status strongly influenced intra-category. Multivariate data reduction of IR spectra using PCA with LDA maximises inter-category variance whilst reducing the influence of intra-class variation towards an objective approach to class cervical cytology based on a biochemical profile.

  12. Prevalence of HPV Infection and Its Association with Cytological Abnormalities of Pap Smears in Tehran

    Directory of Open Access Journals (Sweden)

    M Jamali Zavarei Jamali Zavarei

    2008-09-01

    Full Text Available Background: Human papillomavirus infection is one of the most common genital infections. More than 100 types of this virus have been identified, and most of them are capable of infecting the genital mucosa. Human papillomavirus is in association with cancerous and precancerous lesions of the cervix; some types like HPV 16 and 18 are highly carcinogenic, some types like HPV 31 and 33 are moderately and some types like HPV 6 and 11 are mildly carcinogenic. In this research, the relationship between cytological changes of the squamous epithelial cells and the presence of HPV infections in our cases has been assessed."nMethods: In this prospective study, we collected 681 samples from women admitted to different hospitals and private gynecological clinics in Tehran, during the years 2003-2005. Two specimens were collected from each patient; one for a Pap smear study and the other for PCR assay in order to detect HPV."nResults: Out of our 681 samples, 600 specimens were suitable for PCR assay, and 34 cases were HPV positive in PCR assay. This means that 5.7 percent of our patients were infected with HPV."nConclusion: HPV infection is common in Iran and is nearly identical to European countries such as Germany, and Spain. Also, we found that using PCR assay in order to detect the presence of HPV viruses in vaginal discharges can be very helpful.

  13. Cervical Cytology Smear Combined Colposcopy in the Diagnosis of Cervical Lesions in Clinical Observa-tion%宫颈细胞学涂片联合阴道镜诊断宫颈病变的临床观察

    Institute of Scientific and Technical Information of China (English)

    许盛芳

    2015-01-01

    Objective To explore the cervical cytological smear the clinical effect of combined colposcopy in the di-agnosis of cervical lesions.Methods Between January 2013 and December 2013 in our hospital to accept the cervical cytological smear combined colposcope examination of 880 cases of married women as the research object, on the cervical lesions of inspection, analysis of the clinical condition.Results Of cervical smear cytologic examination and vaginoscope diagnosis coincidence rate in RCI score photogenic compared with significant difference ( P<0.05) , after statistics treat-ment, under colposcopy in the diagnosis of CIN sensitivity was 94.84%, specificity is 88.81%, both the positive pre-dictive value was 72.31% and negative predictive value was 95.63%.Conclusions Cervical cytological smear com-bined colposcope examination before screening of cervical lesions can ensure cerical cancer lesions and early diagnosis of cervical cancer, can also become local screening test for cervical cancer lesion before effective policies.%目的:探讨宫颈细胞学涂片联合阴道镜诊断宫颈病变的临床效果。方法选取2013年1月~2013年12月在我院接受宫颈细胞学涂片联合阴道镜检查的880例已婚妇女作为研究对象,对其进行宫颈病变的检查,分析其临床情况。结果宫颈涂片细胞学检查与阴道镜下RCI评分诊断在符合率上相比较差异具有显著性( P<0.05),经统计学处理后,阴道镜下诊断CIN的敏感性是94.84%,特异性是88.81%,两者联合的阳性预测值是72.31%,阴性预测值是95.63%。结论宫颈细胞学涂片联合阴道镜检查筛选子宫颈病变可以保证宫颈癌前病变和前期宫颈癌的确诊,还可以成为当地筛选检查宫颈癌前病变的有效政策。

  14. Risk assessment on liquid based cytology test and human papilloma virus L1 Nuclecapsid protein detection in the diagnosis of cervical lesions and its progress

    Institute of Scientific and Technical Information of China (English)

    Ling-Zhi Chen

    2016-01-01

    Objective: To discuss the clinical value of liquid based cytology test in the diagnosis of cervical lesions and to evaluate the expression difference and significance of human papilloma virus L1(HPV L1) protein in exfoliative cells of different cervical lesions. Methods: A total of 1 400 cases of patients in gynaecological clinics of our hospital from January 2009 to August 2013 were selected and were given liquid based cytology test(LCT) examination and HPV L1 protein expression detection in exfoliative cells. Meanwhile, cervical biopsy pathology results were taken as baseline for make a comparison. Results: (1) In such1400 cases of women: LCT(+)-30 cases, accounting for 2.1%, HPV L1 protein detection(+)-18 cases, accounting for 1.29%; LCT and HPV L1 protein detection(+)-8 cases, LCT and HPV L1 protein detection(-)- 1360 cases. (2)Pathological tissue biopsy was performed on 30 cases of patients with LCT(+), results showed that(+)were 23 cases; pathological tissue biopsy was performed on 18 cases of patients with HPV L1 protein(+),results showed that (+)were 14 cases. Among 1 360 cases with LCT and HPV L1 protein detection(-), 497 cases were willing to accept the pathological biopsy, and the results showed that 1 case presented(+), accounting for 0.2%. Total 545 cases were willing to accept the pathological biopsy, among whom, 38 cases presented(+), accounting for 6.97%;(3)Sensitivity and specificity of LCT combined with HPV L1 protein detection were higher than single-method detection, and difference had statistical significance. Conclusion: liquid based cytology test combined with HPV L1 protein detection could improve the diagnostic rate of cervical lesions, and were expected as the effective method in the early clinical screening of cervical neoplasia lesions.

  15. Clinical analysis on results of cervical cytology screening: report of 6 498 cases%6498例宫颈液基细胞学检查结果分析

    Institute of Scientific and Technical Information of China (English)

    李茜; 郭德玉; 黄玉胜; 陈艳

    2013-01-01

    Objective To investigate the diagnose accordance rate of liquid-based cytology test ( LCT) and tissue biopsy for cervix, and to investigate the clinical application value of LCT combined with tissue biopsy in early screening for cervical epithelial lesions. Methods The results of 6 498 cases undergoing cervical LCT inspection during January to December 2011 were retrospectively analyzed. Patients with atypical squamous cells of undetermined significance (ASC-US) or severe than ASC-US were defined as abnormal cytology according to Bethesda system for cytology classifications. Among 377 cases of abnormal cytology, 148 cases received biopsy inspection. The cytology and histology results were compared, and the relationship of lesion and ages were analyzed. Results In 6 498 cases receiving cervical LCT, the abnormal cytology ratio was 5. 80%. Among them, ASC-US were 215 cases (57.03% ), atypical squamous cell cannot exclude high grade squamous intraepithelial lesion (ASC-H) were 36 cases (9.55%), low grade squamous intraepithelial lesion (LSIL) were 82 cases (21. 75% ) , high grade squamous intraepithelial lesion ( HSIL) were 39 cases ( 10. 34% ) , atypical glandular cells of undetermined significance not otherwise specified ( AGC-NOS) were 4 cases (1. 06% ), and squamous cell carcinoma (SCC) were 1 cases (0.27% ). Among the 148 cases of biopsy, cases of inflammation reaction were 39 cases (26. 35% ) , koilocytotic cells 16 cases (10. 81% ) , cervical intraepithelial neoplasia (CIN Ⅰ ) 32 cases (21.62% ), CINⅡ35 cases (23.65% ), CINⅢ 17 cases (11.49% ), squamous carcinoma 8 cases (5.41%), and adenocarcinoma 1 cases (0. 68% ) , which revealing that the cytology results had a high conformity rate with biopsy results. And the onset age of cervical lesions ranged from 30 to 50. Conclusion LCT is an important approach of screening for cervical epithelial lesions with its high accuracy. Combination of LCT and tissue biopsy can greatly improve early diagnosis of CIN and

  16. Striatal morphology correlates with sensory abnormalities in unaffected relatives of cervical dystonia patients.

    LENUS (Irish Health Repository)

    Walsh, Richard A

    2012-02-01

    Structural grey matter abnormalities have been described in adult-onset primary torsion dystonia (AOPTD). Altered spatial discrimination thresholds are found in familial and sporadic AOPTD and in some unaffected relatives who may be non-manifesting gene carriers. Our hypothesis was that a subset of unaffected relatives with abnormal spatial acuity would have associated structural abnormalities. Twenty-eight unaffected relatives of patients with familial cervical dystonia, 24 relatives of patients with sporadic cervical dystonia and 27 control subjects were recruited. Spatial discrimination thresholds (SDTs) were determined using a grating orientation task. High-resolution magnetic resonance imaging (MRI) images (1.5 T) were analysed using voxel-based morphometry. Unaffected familial relatives with abnormal SDTs had reduced caudate grey matter volume (GMV) bilaterally relative to those with normal SDTs (right Z = 3.45, left Z = 3.81), where there was a negative correlation between SDTs and GMV (r = -0.76, r(2) = 0.58, p < 0.0001). Familial relatives also had bilateral sensory cortical expansion relative to unrelated controls (right Z = 4.02, left Z = 3.79). Unaffected relatives of patients with sporadic cervical dystonia who had abnormal SDTs had reduced putaminal GMV bilaterally compared with those with normal SDTs (right Z = 3.96, left Z = 3.45). Sensory abnormalities in some unaffected relatives correlate with a striatal substrate and may be a marker of genetic susceptibility in these individuals. Further investigation of grey matter changes as a candidate endophenotype may assist future genetic studies of dystonia.

  17. Psychosocial outcomes of three triage methods for the management of borderline abnormal cervical smears: an open randomised trial

    OpenAIRE

    McCaffery, Kirsten J.; Irwig, Les; Turner, Robin; Chan, Siew Foong; Macaskill, Petra; Lewicka, Mary; Clarke, Judith; Weisberg, Edith; Barratt, Alex

    2010-01-01

    Objective To assess which of three triage strategies for women with borderline abnormal cervical smear results in the best psychosocial outcomes. Design Pragmatic, non-blinded, multicentre, randomised controlled trial. Setting 18 family planning clinics across Australia, covering both urban and rural areas, between January 2004 and October 2006. Participants Women aged 16-70 years (n=314) who attended routine cervical screening and received a borderline cervical smear. Interventions Patients ...

  18. [Sexual profile of women with cervical cytology in a first level unit].

    Science.gov (United States)

    Cabrera-Gaytán, David Alejandro; Palacios-Rodríguez, Raúl Gabriel; Guzmán-Solorio, José Antonio

    2014-01-01

    INTRODUCCIÓN: el perfil sexual de las mujeres conforma un conjunto de componentes que juega un papel fundamental en la aparición del virus del papiloma humano (VPH) o del cáncer cervicouterino (CACU). Se buscó determinar el perfil sexual de un grupo de mujeres con citología cervical en una unidad de primer nivel del Instituto Mexicano del Seguro Social del Estado de México. MÉTODOS: se estudiaron mujeres que acudieron para detección citológica de tumor maligno del cuello uterino. Se les entrevistó para obtener características sociodemográficas y ginecoobstétricas. El análisis estadístico se hizo con prueba exacta de Fisher, chi cuadrada de Mantel-Haenszel, y correlación de Spearman.

  19. Screening results on cervical lesions with DNA quantitative cytology and liquid-based cytology%DNA定量细胞学配合液基细胞学对宫颈病变筛查的价值

    Institute of Scientific and Technical Information of China (English)

    曹红英; 武卫华; 许振; 许艳梅

    2011-01-01

    OBJECTIVE: To evaluate the value of the DNA quantitative cytology with liquid-based cytology in cervical cancer prevention and treatment. METHODS: 4 352 cases of patients in our hospital outpatient gynecologic from 01-01-2009 to 10-31-2010 were involved in this study. All the 504 cases which were recommended biopsy were conducted colposcopy and biopsy. The pathological changes were observed after the routine HE staining. Then the positive coincidence rate of the liquid-based cytology was calculated with routine HE staining and DNA Feulgen staining with routine HE staining respectively. RESULTS: The positive rate was 52. 18% (263/504) with the diagnosis of the TBS biopsy criteria. The positive rate was 66.67%(336/504) with the diagnosis of the DNA Feulgen staining biopsy criteria, While the positive rate was 81.75 % (412/504) with the diagnosis of the two combined cytological method biopsy criteria. There was significant difference among the three methods (P<0. 05). CONCLUSIONS: DNA Feulgen staining compared with the single liquid-based cytology, the rate of early detection of cervical lesions is improved significantly. The false negative rate of thc liquid-based cytology can be reduced by the combination of the two, but also the detection rate of cervical lesions of early can be improved. It plays a posive role in order to prevent further development of lesions of early cervical cancer.%目的:评价DNA定量细胞学配合液基细胞学检查在宫颈癌防治中的价值.方法:收集2009-01-01-2010-10-31在我院妇科门诊行液基细胞学及DNA定量检查的患者4 352例,对检查结果建议为活检的病例504例行阴道镜检查及活体组织检查,常规HE染色后观察病变程度,分别计算其与液基细胞学和DNA Feulgen染色后的阳性率.结果:以TBS活检标准行活检病例的阳性检出率为52.18%(263/504),以DNA定量分析结果活检标准行活检的病例阳性检出率为66.67%(336/504),经2种细胞学方法联

  20. HPV-Based Screening, Triage, Treatment, and Followup Strategies in the Management of Cervical Intraepithelial Neoplasia

    OpenAIRE

    Oscar Peralta-Zaragoza; Jessica Deas; Claudia Gómez-Cerón; Wendy Argelia García-Suastegui; Geny del Socorro Fierros-Zárate; Nadia Judith Jacobo-Herrera

    2013-01-01

    Cervical cancer is the second most common cause of death from cancer in women worldwide, and the development of new diagnostic, prognostic, and treatment strategies merits special attention. Many efforts have been made to design new drugs and develop immunotherapy and gene therapy strategies to treat cervical cancer. HPV genotyping has potentially valuable applications in triage of low-grade abnormal cervical cytology, assessment of prognosis and followup of cervical intraepithelial neoplasia...

  1. The clinical impact of using p16(INK4a) immunochemistry in cervical histopathology and cytology: an update of recent developments.

    Science.gov (United States)

    Bergeron, Christine; Ronco, Guglielmo; Reuschenbach, Miriam; Wentzensen, Nicolas; Arbyn, Marc; Stoler, Mark; von Knebel Doeberitz, Magnus

    2015-06-15

    Cervical cancer screening test performance has been hampered by either lack of sensitivity of Pap cytology or lack of specificity of Human Papillomavirus (HPV) testing. This uncertainty can lead to unnecessary referral and treatment, which is disturbing for patients and increases costs for health care providers. The identification of p16(INK4a) as a marker for neoplastic transformation of cervical squamous epithelial cells by HPVs allows the identification of HPV-transformed cells in histopathology or cytopathology specimens. Diagnostic studies have demonstrated that the use of p16(INK4a) immunohistochemistry substantially improves the reproducibility and diagnostic accuracy of histopathologic diagnoses. p16(INK4a) cytology has substantially higher sensitivity for detection of cervical precancer in comparison to conventional Pap tests. Compared to HPV DNA tests, immunochemical detection of p16(INK4a) -stained cells demonstrates a significantly improved specificity with remarkably good sensitivity. About 15 years after the initial observation that p16(INK4a) is overexpressed in HPV-transformed cells we review the accumulated clinical evidence suggesting that p16(INK4a) can serve as a useful biomarker in the routine diagnostic work up of patients with HPV infections and associated lesions of the female anogenital tract.

  2. DIAGNOSTIC AND THERAPEUTIC POSSIBILITIES IN THE PROPHYLAXIS OF CERVICAL CANCER

    Directory of Open Access Journals (Sweden)

    Marzena Wrześniewska

    2013-11-01

    Full Text Available Poland is one of the countries with high cervical cancer morbidity and mortality. The main means to change this situation is to manage an active and modern programme of cervical cancer prophylaxis and diagnostics. To a large extent, the effectiveness of a cervical cancer prophylaxis programme is decided by the availability of modern diagnostic research. The conventional Papanicolaou test and modern LBC cytology techniques were discussed in the article, taking into consideration HPV diagnostics in the procedures for carefully selected cytological diagnosis, in the so called in-depth stage of preventive screening tests and the role of the p16 biomarker in predicting the development of a higher degree of epithelial-cell pathologies of the cervix. Colposcopy as a diagnostic method for the verification of cytological and virological abnormalities. The modern LEEP/LLETZ procedure used in diagnosis and treatment of cervical changes is used to realise the in-depth stage of cervical cancer prophylaxis programmes.

  3. Research of cervical cytology screening in diagnosis of 370,000 cases with cervical lesion in Guangdong%广东地区37万例妇女子宫颈细胞学筛查子宫颈病变的研究

    Institute of Scientific and Technical Information of China (English)

    Baowen Zheng; Congde Chen; Anxiang Wei; Han Ran; Dong Liu; Wenling Yue; Huirong Zhou; Qingyi Zhu; Juming Su

    2009-01-01

    Objective:Analysis of the results of 37 million cases by using cervical liquid-based cytology screening.discussion the situation of cervical cancer and precancerous lesions in Guangdong province.Methods:From 2002 to 2006.using liquid-based thin-layer cytology (TCT)method screening cervical lesions within 371.929 women in Guangdong.Cytological diagnosis adopt TBS(the Bethesda system)Standard(TBS improved 2001 version of the diagnostic criteria),staastic positive rate by age and region respectively.Results:371,929 cases of gynecology cervical samples in Guangdong.331,251 cases were negative,accounted for 91.23%:cytology positive cases:32,548 cases were squamous intreepithelial lesions(8.96%),486 cases were glandular epithelial lesions(0.13%):grouped according to age:30 to 40-year-old positive rate was 9.13%,40 to 50-year-old positive rate was 9.60%.the latter had higher cytology positive rate;grouped according to region:the total samples in PRD areas were 304.951 cases,accounted for 81.99%,in which 24301 cases were positive(8.14%),in 66978 cases(18.01%)of NON-PRD regions,7645 cases were positive(11.87%),there were 1858 cases had biopsy/follow-up results from 2004t02005.and the rate was 2.88%.Conclusion:The cytological positive rate of cervical cancer and its pre-cancerous lesions was 9.09%in Guangdong(of which the rate of cytological diagnosis as precancerous lesions was 3.40%),30 to 50 age group had the high incidence of cervical cancer and its precursor lesions.and also the main stage of prevention for cervical cancer.Cytology positive rate was 11.87% in economic underdevelopment Non-Pead River Delta regions.much higher than the Pearl River Delta region.

  4. Chlamydia trachomatis detection in cervical PreservCyt specimens from an Irish urban female population.

    LENUS (Irish Health Repository)

    Keegan, H

    2012-02-01

    OBJECTIVE: The aim of this study was to determine the prevalence of cervical Chlamydia trachomatis infection by polymerase chain reaction (PCR) in urban women undergoing routine cervical cytological screening and to investigate the relationship with age, cytology, smoking status and concurrent human papillomavirus (HPV) infection. METHODS: A total of 996 women (age range 16-69 years) attending general practitioners for routine liquid-based cervical smear screening in the Dublin area were recruited in the study of prevalence of C. trachomatis. Informed consent was obtained and liquid-based cytology (LBC) specimens were sent for cytological screening. DNA was extracted from residual LBC and tested for C. trachomatis by PCR using the highly sensitive C. trachomatis plasmid (CTP) primers and for HPV infection using the MY09\\/11 primers directed to the HPV L1 gene in a multiplex format. RESULTS: The overall prevalence of C. trachomatis was 5.4%. Prevalence was highest in the <25 years age group (10%). Coinfection with HPV and C. trachomatis occurred in 1% of the screening population. A higher rate of smoking was observed in women positive for C. trachomatis, HPV infections or those with abnormal cervical cytology. Chlamydia trachomatis infection was not associated with abnormal cytology. CONCLUSIONS: Women (5.4%) presenting for routine cervical screening are infected with C. trachomatis. Opportunistic screening for C. trachomatis from PreservCyt sample taken at the time of cervical cytological screening may be a possible strategy to screen for C. trachomatis in the Irish female population.

  5. Fine-needle aspiration cytology in nonpalpable mammographic abnormalities in breast cancer screening: results from the breast cancer screening programme in Oslo 1996-2001.

    Science.gov (United States)

    Sauer, Torin; Myrvold, Kristina; Lømo, Jon; Anderssen, Karin Yvonne; Skaane, Per

    2003-10-01

    Fine-needle aspiration cytology (FNAC) of nonpalpable mammographic lesions has been under attack from two sides for some years. There has been much discussion and controversy as to the ability to differentiate between in situ and invasive carcinomas in cytological material. A further issue is that of optimal sampling to obtain adequate cell material in sufficient quantity. We present the results of FNAC from 832 nonpalpable mammographic abnormalities detected in the course of the breast cancer screening programme in Oslo during 1996-2001. In 11.6% of cases the smears were inadequate, and there were 7% false negatives (FN) and 1.3% false positives. Of the FN, 64% represented microcalcifications and 86% were due to sampling errors. Absolute sensitivity was 74%, complete sensitivity 88% and specificity 88%. In 255 carcinomas a cytological diagnosis of them as in situ or invasive was made. In 93% of the invasive cases (190/205) these had been correctly identified as invasive on FNAC. In 78% of cases proper follow-up could be resolved by cytology/radiology alone. Suboptimal sampling and localization remains the main cause of FN FNAC results. Problems in differentiating between in situ and invasive breast carcinomas can be significantly reduced by applying strict criteria for in situ lesions. PMID:14659146

  6. The Application Value of Colposcopy Combined with Cervical Cytology in Cervical Lesions%阴道镜检查结合宫颈细胞学在宫颈病变中的应用价值

    Institute of Scientific and Technical Information of China (English)

    王少华

    2014-01-01

    目的:探讨阴道镜检查结合宫颈细胞学在宫颈病变诊治中的临床应用价值。方法随机选在该院接收的656例行阴道镜检查结合宫颈细胞学的妇女作为研究对象,对所有对象的资料进行回顾性分析,总结患者的治疗效果。结果单纯行阴道镜检查筛查3例,无漏诊,癌前期可疑1例。单纯行新柏氏液基细胞学检测检查筛选宫颈癌4例,漏诊1例。行阴道镜检查结合新柏氏液基细胞学检测符合宫颈癌病例5例,无漏诊。结论单纯性阴道镜检查或者新柏氏液基细胞学检测宫颈癌存在漏诊情况,而结合两种方法进行检查后无漏诊现象,即结合两种方法诊断早期宫颈癌有助于提高疾病检出率,值得在临床上推广和使用。%Objective To investigate the clinical application value of colposcopy combined with cervical cytology in the diagnosis and treatment of cervical lesions. Methods 656 cases of women underwent colposcopy combined with cervical cytology in our hos-pital from March, 2012 to March, 2013 were randomly selected as the subjects, and the data of them were analyzed retrospectively, the treatment effect was summarized. Results A simple colposcopy screening 3 cases, no missed diagnosis, pre-cancerous suspi-cious 1 case. Simple ThinPrep liquid-based cervical cytology screening examination in 4 cases, missed 1 case. Colposcopy com-bined with ThinPrep liquid-based cervical cytology in line 5 cases, no missed case. Conclusion Missed diagnosis exists in simple colposcopy or ThinPrep liquid-based cervical cytology for detecting cervical cancer, but that does not exist if combining the two methods for detecting cervical cancer, and the diagnosis of early cervical cancer by combining the two methods is conducive to im-proving the detection rate of the disease, which is worthy of clinical popularization and application.

  7. 6000例宫颈液基细胞学检查的病理分析%Pathological Analysis on 6000 Cases of Cervical Thinprep Cytological Test

    Institute of Scientific and Technical Information of China (English)

    郝彤; 李晓敏; 马潇潇; 刘学伟; 何金萍; 刘晓燕

    2013-01-01

    Objective:Compare detection rate of cervical cancer lession precancerous 、degree of satisfaction of the examples and coincidence of biopsy masccline by liquid-based cytology test and traditional Bethesda smeara.To explore apply value of liquid-based cytology test in the screening of cervical cancer .Methods:Analyse resultes of liquid-based cytology test of six thousands married women in Ren Qiu area.Review resultes of traditional Bethesda smeara of five thousands women from 2008-2010.Compare the two.And compare cervical biopsy of masccline cases.Results:TCT cervical biopsy CINI 69 cases、CINII 16 cases、CINIII 42 cases. detection rate of cervical cancer lession precancerous 、degree of satisfaction of examples and the coincidence of biopsy masccline are 96.02%、2.11%、62.56%.Control group cervical biopsy CINI 23 cases、CINII 5 cases、CINIII 9 cases. detection rate of cervical cancer lession precancerous 、degree of satisfaction of the examples and coincidence of biopsy masccline are 62.7%、0.62%、38.54%.Conclusion:liquid-based cytology test is bet er than traditional Bethesda smeara in the above three indexes. It can improve diagnosis rate of prophase cervix lesion、reduce incidence rate of cervical cancer and promote woman reproductive health.%目的:通过将液基细胞学(TCT)检查与传统巴氏涂片检查对妇科宫颈癌癌前病变的检出率、标本满意度及活检阳性符合率进行对比,探讨TCT在妇科宫颈病变筛查中的应用价值。方法:对任丘地区6000例已婚妇女应用液基细胞学检查结果进行分析,并回顾2008-2010年5000例应用传统巴氏涂片的妇科检查结果,二者对比,并对细胞学检查阳性病例行宫颈活检对照。结果:TCT组宫颈活检CINI 69例,CINII 16例,CINIII 42例,标本满意度和宫颈癌癌前病变的检出率分别是96.02%和2.11%,活检阳性符合率为62.56%;对照组宫颈活检CINI 23例,CINII 5例,CINIII 9例,标本满

  8. Analysis on liquid-based cytology test in the screening of cervical cancer%宫颈癌筛查中液基细胞学研究

    Institute of Scientific and Technical Information of China (English)

    王赫

    2009-01-01

    目的:评价液基细胞学(LPT)薄片制片对宫颈癌筛查的准确性.方法:对3 600例和2 200例受检者分别进行LPT制片和传统宫颈细胞涂片法进行宫颈癌筛查.细胞诊断采用TBS分级系统,阳性诊断包括意义不明的不典型鳞状上皮(ASCUS)以上病变.所有ASCUS以上病变的受检者全部在阴道镜下活检.结果:LPT薄片法和传统涂片法对ASCUS以上病变检出阳性率分别为10.5%和5.2%,两种检测方法的阳性率差异有统计学意义(P<0.01);两种制片方法的阳性结果与病理检查符合率比较:LPT薄片法检出SCC、HSIL、LSIL与阴道镜活检阳性符合率分别为100%、91%、82%,传统宫颈巴氏涂片法检出SCC、HSIL、LSIL与阴道镜活检阳性符合率分别为100%、60%、53%,两种方法比较差异有统计学意义(P<0.05).结论:LPT液基细胞学检查敏感性明显高于传统宫颈细胞涂片法,能大大提高检出率,尤其是对宫颈癌前筛查的患者.%Objective: To evaluate the accuracy of liquid-based cytology test in the screening of cervical cancer. Methods: 3 600 and 2 200 subjects were screened for cervical cancer by liquid-based cytology test and traditional cervical cells smears, respectively. Cell diagnosis adopted TBS-group system, positive diagnosis included above-ASCUS pathological changes. All the subjects of above-ASCUS pathological changes were examined under colposcopo. Results: The detection rates of above-ASCUS pathological changes identified by liq-uid-based cytology test and traditional smears were 10. 5% and 5.2 %, respectively (P < 0. 01) . The coincidence rates of SCC, HSIL and LSIL detected by liquid-based cytology test and biopsy under eolposcepe were 100%, 91% and 82%, respectively, while the coinci-dence rates detected by traditional cervical cells smears and biopsy under colposcope were 100%, 60% and 53%, respectively (P <0. 05)Conclusion: The sensitivity and detection rate of liquid-based cytology test are higher than those

  9. CO2 laser vaporization in the treatment of cervical human papillomavirus infection in women with abnormal Papanicolaou smears

    DEFF Research Database (Denmark)

    Ruge, S; Felding, C; Skouby, S O;

    1992-01-01

    In a randomized study, we have evaluated the treatment of cervical human papillomavirus (HPV) lesions by CO2 laser vaporization. Fifty patients with abnormal Papanicolaou smears and histological evidence of cervical HPV infection associated or not with cervical intraepithelial neoplasia (CIN) gra...... in their cervical smears at 12 months' follow-up was identical in the two groups, supporting the hypothesis that HPV is a persistent infection during which the virus is widespread in the vaginal epithelium.......In a randomized study, we have evaluated the treatment of cervical human papillomavirus (HPV) lesions by CO2 laser vaporization. Fifty patients with abnormal Papanicolaou smears and histological evidence of cervical HPV infection associated or not with cervical intraepithelial neoplasia (CIN) grade...... I were randomized to either a treatment or a control group. The cervical swabs were obtained every 3 months in both groups and examined for HPV type 16 DNA by the polymerase chain reaction. After a follow-up period of 12 months no significant differences were found between the laser treatment...

  10. Cervical cancer screening in the Faroe Islands

    DEFF Research Database (Denmark)

    Hammer, Turið; Lynge, Elsebeth; Djurhuus, Gisela W;

    2015-01-01

    aim was to provide the first description of cervical cancer screening, and to determine the screening history of women diagnosed with cervical cancer in the Faroe Islands. MATERIAL AND METHODS: Screening data from 1996 to 2012 were obtained from the Diagnostic Centre at the National Hospital......BACKGROUND: The Faroe Islands have had nationally organised cervical cancer screening since 1995. Women aged 25-60 years are invited every third year. Participation is free of charge. Although several European overviews on cervical screening are available, none have included the Faroe Islands. Our...... 1999. At present, 7.0% of samples have abnormal cytology. Of all ASCUS samples, 76-95% were tested for HPV. A total of 58% of women diagnosed with cervical cancer did not participate in screening prior to their diagnosis, and 32% had normal cytology in the previous four years. CONCLUSION: Despite...

  11. HPV E6/E7 mRNA testing is more specific than cytology in post-colposcopy follow-up of women with negative cervical biopsy.

    Directory of Open Access Journals (Sweden)

    Sveinung Wergeland Sørbye

    Full Text Available BACKGROUND: In Norway, women with negative or low-grade cervical biopsies (normal/CIN1 are followed up after six months in order to decide on further follow-up or recall for screening at three-year intervals. A high specificity and positive predictive value (PPV of the triage test is important to avoid unnecessary diagnostic and therapeutic procedures whereas a low risk of high-grade disease among triage negative women assures safety. MATERIALS AND METHODS: At the University Hospital of North Norway, cytology and the HPV mRNA test PreTect HPV-Proofer, detecting E6/E7 mRNA from HPV types 16, 18, 31, 33 and 45, are used in post-colposcopy follow-up of women with negative or low-grade biopsy. In this study, women with negative biopsy after high grade cytology (ASC-H/HSIL and/or positive HPV mRNA test in the period 2005-2009 were included (n = 520. Histologically confirmed cervical intraepithelial neoplasia of grade 2 or worse (CIN2+ was used as study endpoint. RESULTS: Of 520 women with negative or low-grade biopsy, 124 women (23.8% had CIN2+ in follow-up biopsy. The sensitivity and specificity of the HPV mRNA test were 89.1% (95% CI, 80.1-98.1 and 92.5% (95% CI, 88.2-96.7, respectively. The ratios of sensitivity, specificity and PPV of HPV mRNA testing compared to repeat cytology for finding CIN2+ was 1.05 (95% CI: 0.92-1.21, 1.21 (95% CI: 1.12-1.32, and 1.49 (95% CI: 1.20-1.86, respectively. The PPV of mRNA was 77.3% (95% CI, 59.8-94.8 in women aged 40 or older. CONCLUSION: Women with negative cervical biopsy require follow-up before resumption of routine screening. Post-colposcopy HPV mRNA testing was as sensitive but more specific than post-colposcopy cytology. In addition, the HPV mRNA test showed higher PPV. A positive mRNA test post-colposcopy could justify treatment in women above 40 years.

  12. Absent cervical spine pedicle and associated congenital spinal abnormalities - a diagnostic trap in a setting of acute trauma: case report

    International Nuclear Information System (INIS)

    Congenital spinal abnormalities can easily be misdiagnosed on plain radiographs. Additional imaging is warranted in doubtful cases, especially in a setting of acute trauma. This patient presented at the emergency unit of our university hospital after a motor vehicle accident and was sent to our radiology department for imaging of the cervical spine. Initial clinical examination and plain radiographs of the cervical spine were performed but not conclusive. Additional CT of the neck helped establish the right diagnosis. CT as a three-dimensional imaging modality with the possibility of multiplanar reconstructions allows for the exact diagnosis and exclusion of acute traumatic lesions of the cervical spine, especially in cases of doubtful plain radiographs and when congenital spinal abnormalities like absent cervical spine pedicle with associated spina bifida may insinuate severe trauma

  13. p16INK4a immunocytochemistry versus human papillomavirus testing for triage of women with minor cytologic abnormalities: a systematic review and meta-analysis.

    Science.gov (United States)

    Roelens, Jolien; Reuschenbach, Miriam; von Knebel Doeberitz, Magnus; Wentzensen, Nicolas; Bergeron, Christine; Arbyn, Marc

    2012-10-25

    The best method for identifying women who have minor cervical lesions that require diagnostic workup remains unclear. The authors of this report performed a meta-analysis to assess the accuracy of cyclin-dependent kinase inhibitor 2A (p16(INK4a)) immunocytochemistry compared with high-risk human papillomavirus DNA testing with Hybrid Capture 2 (HC2) to detect grade 2 or greater cervical intraepithelial neoplasia (CIN2+) and CIN3+ among women who had cervical cytology indicating atypical squamous cells of undetermined significance (ASC-US) or low-grade cervical lesions (LSIL). A literature search was performed in 3 electronic databases to identify studies that were eligible for this meta-analysis. Seventeen studies were included in the meta-analysis. The pooled sensitivity of p16(INK4a) to detect CIN2+ was 83.2% (95% confidence interval [CI], 76.8%-88.2%) and 83.8% (95% CI, 73.5%-90.6%) in ASC-US and LSIL cervical cytology, respectively, and the pooled specificities were 71% (95% CI, 65%-76.4%) and 65.7% (95% CI, 54.2%-75.6%), respectively. Eight studies provided both HC2 and p16(INK4a) triage data. p16(INK4a) and HC2 had similar sensitivity, and p16(INK4a) has significantly higher specificity in the triage of women with ASC-US (relative sensitivity, 0.95 [95% CI, 0.89-1.01]; relative specificity, 1.82 [95% CI, 1.57-2.12]). In the triage of LSIL, p16(INK4a) had significantly lower sensitivity but higher specificity compared with HC2 (relative sensitivity, 0.87 [95% CI, 0.81-0.94]; relative specificity, 2.74 [95% CI, 1.99-3.76]). The published literature indicated the improved accuracy of p16(INK4a) compared with HC2 testing in the triage of women with ASC-US. In LSIL triage, p16(INK4a) was more specific but less sensitive.

  14. p16INK4a immunocytochemistry versus HPV testing for triage of women with minor cytological abnormalities: A systematic review and meta-analysis

    Science.gov (United States)

    Roelens, Jolien; Reuschenbach, Miriam; von Knebel-Doeberitz, Magnus; Wentzensen, Nicolas; Bergeron, Christine; Arbyn, Marc

    2014-01-01

    Background The best method to identify women with minor cervical lesions that require diagnostic work-up remains unclear. We performed a meta-analysis to assess the accuracy of p16INK4a immunocytochemistry compared to hrHPV DNA testing with hybrid capture II (HC2) to detect cervical intraepithelial neoplasia (CIN2+ and CIN3+) in women with a cervical cytology showing atypical squamous cells of undetermined significance (ASC-US) or low-grade cervical lesions (LSIL). Methods A literature search was performed in three electronic databases to identify studies eligible for this meta-analysis. Results Seventeen studies were included in the meta-analysis. The pooled sensitivity of p16INK4a to detect CIN2+ was 83.2% (95%CI: 76.8–88.2%) and 83.8% (95%CI: 73.5–90.6%) in ASC-US and LSIL cervical cytology respectively; pooled specificities were 71.0% (95%CI: 65.0–76.4%) and 65.7% (95%CI: 54.2–75.6%). Eight studies provided both HC2 and p16INK4a triage data. p16INK4a and HC2 have a similar sensitivity and p16INK4a has significantly higher specificity in the triage of women with ASC-US (relative sensitivity: 0.95 (95%CI: 0.89–1.01); relative specificity: 1.82 (95%CI: 1.57–2.12)). In the triage of LSIL, p16INK4a has a significantly lower sensitivity but higher specificity compared to HC2 (relative sensitivity: 0.87 (95%CI: 0.81–0.94); relative specificity: 2.74 (1.99–3.76)). Conclusion The published literature indicates an improved accuracy of p16INK4a compared to HC2 testing in the triage of ASC-US. In LSIL triage p16INK4a is more specific but less sensitive. PMID:22700382

  15. Reproducibilidad en la lectura de un set de placas de citología cérvico-uterinas en cuatro centros especializados de Medellín, Antioquia / Reproducibility of reading a set of cervical cytology smears in four specialized centers in Medellin, Antioquia

    Directory of Open Access Journals (Sweden)

    Edwin Guevara

    2014-05-01

    Full Text Available Objetivo: evaluar el grado de reproducibilidad en la lectura de placas de citología cérvico-uterina entre cuatro centros de lectura especializados de la ciudad de Medellín, Colombia. Metodología: 181 placas de citología cérvico-uterina provenientes de un estudio de prevalencia de anormalidades citológicas en Pueblorrico, Antioquia, se sometieron a lectura en cuatro centros especializados de Medellín. Se le pidió a cada centro que realizara una lectura rutinaria manteniendo el estudio en ciego por medio de recodificación de las placas, evitando que se filtrara información entre centros. Se calculó la concordancia general y el índice kappa de Fleiss. Resultados: de las 181 placas, sólo en 55 placas los 4 centros concordaron en el resultado, obteniéndose un porcentaje de concordancia global del 30% y un índice kappa global de 0,31. Según la escala de Fleiss, se observó una baja reproducibilidad en la lectura de las placas citológicas entre los cuatro centros involucrados en el estudio. La concordancia por pares de centros presentó índices de kappa entre 0,3 a 0,7. Discusión: existe una alta variabilidad en la interpretación de los resultados citológicos entre los centros estudiados. Es necesario implementar procesos de entrenamiento y unificación de criterios de lecturas de la citología cervical en nuestro medio Objective: to assess the degree of reproducibility in the reading of cervical cytology smears among four specialized reading centers at Medellin, Colombia. Methodology: 181 cervical cytology smears from a study on the prevalence of cytological abnormalities in Pueblorrico, a town located in the southwestern region of the state of Antioquia in Colombia, were re-read by four specialized centers in Medellin. Each center was asked to conduct a blind routine reading of the smears to avoid disclosure of information between centers. The reproducibility was measured by percentage agreement and kappa. Results: of 181 smears

  16. Cost-effectiveness of conventional cytology and HPV DNA testing for cervical cancer screening in Colombia Costo-efectividad de la citología y la tamización con pruebas de ADN-VPH para cáncer de cuello uterino en Colombia

    OpenAIRE

    Oscar Andrés-Gamboa; Liliana Chicaíza; Mario García-Molina; Jorge Díaz; Mauricio González; Raúl Murillo; Mónica Ballesteros; Ricardo Sánchez

    2008-01-01

    OBJECTIVE: To assess cost-effectiveness of conventional cytology and HPV DNA testing for cervical-cancer screening in Colombia. MATERIAL AND METHODS: The National Cancer Institute of Colombia (NCIC) in 2007 developed a Markov model on the natural history of cervical cancer; no screening, conventional cytology, and HPV DNA testing were compared. Only direct costs were used. Outcomes comprise cervical cancer mortality, years of life saved, and lifetime costs. Discounted incremental cost-effecti...

  17. Effects of streamlining cervical cancer screening the Dutch way: consequences of changes in the Dutch KOPAC-based follow-up protocol and consensus-based limitation of equivocal cytology

    DEFF Research Database (Denmark)

    Briët, Martijn C; Berger, Thomas H D; van Ballegooijen, Marjolein;

    2010-01-01

    To analyze the impact of the 1995 revision of the Dutch cervical screening program guidelines (e.g., the introduction of more stringent criteria for cytologic diagnosis of atypical squamous cells of undetermined significance [ASCUS]) on the negative side effects of screening in Region West....

  18. Evaluation of Brain and Cervical MRI Abnormality Rates in Patients With Systemic Lupus Erythematosus With or Without Neurological Manifestations

    International Nuclear Information System (INIS)

    Central nervous system (CNS) involvement has been observed in 14-80% of patients with systemic lupus erythematosus (SLE). Magnetic resonance imaging (MRI) is an appropriate method for evaluating CNS involvement in these patients. Clinical manifestations and MRI findings of CNS lupus should be differentiated from other mimicking diseases such as multiple sclerosis (MS). The aim of this study was to evaluate the prevalence and extent of brain and cervical cord MRI lesions of lupus patients. The relationship between neurological signs and symptoms and MRI findings were evaluated as well. Fifty SLE patients who had been referred to the rheumatology clinic of our hospital within 2009 were included in a cross sectional study. All patients fulfilled the revised 1981 American College of Rheumatology (ACR) criteria for SLE. We evaluated the neurological signs and symptoms and brain and cervical MRI findings in these patients. Forty-one patients (82%) were female and nine (18%) were male. The mean age was 30.1 ± 9.3 years. Twenty eight (56%) patients had an abnormal brain MRI. No one showed any abnormality in the cervical MRI. The lesions in 20 patients were similar to demyelinative plaques. Seventeen patients with abnormal brain MRI were neurologically asymptomatic. There was only a significant relationship between neurological motor manifestations and brain MRI abnormal findings. Unlike the brain, cervical MRI abnormality and especially asymptomatic cord involvement in MRI is quite rare in SLE patients. This finding may be helpful to differentiate SLE from other CNS disorders such as MS

  19. Frequency and patterns of abnormal Pap smears in Sudanese women with infertility: What are the perspectives?

    Directory of Open Access Journals (Sweden)

    Ahmed O Almobarak

    2013-01-01

    Conclusions: Epithelial cell abnormalities are significantly higher in women with infertility as compared with fertile women. Importantly, inflammatory smears were reported two times more than in the controls. We recommend pap smear as a routine practice for all women assessed for infertility problems. Further studies are necessary to evaluate the incidence of human papilloma virus infections in infertile women with abnormal cervical cytology.

  20. 薄层液基细胞学在宫颈癌及其癌前病变筛查中的价值%The value of Thin prep cytology test in cervical precancerous lesions and cervical cancer screening

    Institute of Scientific and Technical Information of China (English)

    雷静; 万霖; 李惠新; 蒋瑛; 李瑞萍

    2011-01-01

    Objective: To evaluate the detecting technology of thin layers of liquid base cytology (TCT) on the diagnosis of precancerous lesions of uterine cervix and the clinical value, accuracy of the cervical cancer screening. Methods: To collect and analysis 7340 patients who were did examine of TCT in gynecological clinic line of our hospital from May, 2009 ~ November, 2010. Take cytology diagnosed ASC - US and higher as positive results, and the positive results do pathohistological. Take histological diagnosis as gold standard. Results: Thin prep cytology test specimens of SCC, and satisfaction LSIL accuracy HSIL, respectively, 76.8% 97.3%, 100%. Conclusion: TCT combination diagnostic systems of TBS are ideal method of current diagnosis precancerous lesions of uterine cervix (CIN) and cervical cancer screening. Also it can be used as an indicator for cervical cancer detecting. There is some risk of precancerous lesions of young In ASC-US patients.%目的:评价薄层液基细胞学(Thin prep cytology test,TCT)检测技术对宫颈癌前病变的诊断和宫颈癌筛查的准确性及临床价值.方法:收集分析2009年5月~2010年11月在我院妇科门诊行TCT检查的受检者7340例,以细胞学诊断为未明确意义的不典型鳞状上皮细胞(ASC-US)及以上者为阳性结果,并对阳性结果行病理组织学诊断,以组织学诊断作为金标准.结果:液基细胞学标本满意度高,对SCC、HSIL、LSIL的准确率分别为76.8%、97.3%、100%.结论:TCT结合TBS诊断系统是目前诊断宫颈癌前病变和筛查宫颈癌的理想方法 [1],同时也可以作为一项宫颈癌术后随访的检测指标 [2].ASC-US患者中存在部分年轻的高危癌前病变者.

  1. The effectiveness of acetic acid wash protocol and the interpretation patterns of blood contaminated cervical cytology ThinPrep® specimens

    Directory of Open Access Journals (Sweden)

    Nora K Frisch

    2015-01-01

    Full Text Available Background: ThinPrep® (TP cervical cytology, as a liquid-based method, has many benefits but also a relatively high unsatisfactory rate due to debris/lubricant contamination and the presence of blood. These contaminants clog the TP filter and prevent the deposition of adequate diagnostic cells on the slide. An acetic acid wash (AAW protocol is often used to lyse red blood cells, before preparing the TP slides. Design: From 23,291 TP cervical cytology specimens over a 4-month period, 2739 underwent AAW protocol due to initial unsatisfactory smear (UNS with scant cellularity due to blood or being grossly bloody. Randomly selected 2739 cervical cytology specimens which did not undergo AAW from the same time period formed the control (non-AAW group. Cytopathologic interpretations of AAW and non-AAW groups were compared using the Chi-square test. Results: About 94.2% of the 2739 cases which underwent AAW were subsequently satisfactory for evaluation with interpretations of atypical squamous cells of undetermined significance (ASCUS 4.9% (135, low-grade squamous intraepithelial lesions (LSIL 3.7% (102, and high-grade squamous intraepithelial lesions (HSIL 1% (28. From the 2739 control cases, 96.3% were satisfactory with ASCUS 5.5% (151, LSIL 5.1% (139, and HSIL 0.7% (19. The prevalence of ASCUS interpretations was similar (P = 0.33. Although there were 32% more HSIL interpretations in the AAW group (28 in AAW vs. 19 in non-AAW, the difference was statistically insignificant (P = 0.18. AAW category; however, had significantly fewer LSIL interpretations (P = 0.02. The percentage of UNS cases remained higher in the AAW group with statistical significance (P < 0.01. Conclusions: While AAW had a significantly higher percent of UNS interpretations, the protocol was effective in rescuing 94.2% of specimens which otherwise may have been reported unsatisfactory. This improved patient care by avoiding a repeat test. The prevalence of ASCUS and HSIL

  2. 宫颈细胞学阴性HPV阳性妇女的HPV分型检测%HPV genotyping detection of HPV positive women with negative cervical cytology

    Institute of Scientific and Technical Information of China (English)

    张珊; 洪颖; 马莉; 兰明

    2015-01-01

    目的:探讨宫颈细胞学阴性人乳头瘤病毒( HPV)阳性妇女接受HPV分型检测的临床意义。方法宫颈细胞学阴性HPV阳性的妇女200例,对其进行HPV分型检测并转诊阴道镜及组织病理学检查,分析感染HPV型别与宫颈上皮内瘤变( CIN)Ⅱ+的关系,并在受检1 a后对病理检查结果为CINⅠ及宫颈炎症的患者进行随访,随访方法为宫颈新柏氏液基细胞学检查及 HPV 分型检测。结果200例受检者中检出 CIN Ⅱ+患者37例(18.6%),HPV16型感染者发生CINⅡ+的风险是不伴HPV16型感染者的2.608倍(OR值为2.608,P<0.05)。151例完成随访的CIN Ⅰ及宫颈炎症患者中,同型HPV感染者的宫颈上皮内瘤变患病率显著高于非同型感染者(P<0.01),且同型感染者发生病理高级别进展的风险是非同型感染患者的14.89倍。结论 HPV分型检测对宫颈细胞学阴性HPV阳性妇女有重要的临床意义。宫颈细胞学阴性HPV16型感染者应立即转诊阴道镜检查。 HPV分型检测用于细胞学阴性HPV阳性妇女的随访,随访中发现同型HPV阳性的患者应转诊阴道镜检查,尤其HPV16型感染患者。%Objective To investigate the clinical significance of HPV genotyping detection in patients with negative cervi-cal cytology and positive human papilloma virus (HPV). Methods A total of 200 women with negative cervical cytology and positive HPV received the HPV genotyping detection and were referred to colposcopy and histopathological examination. The cor-relation between different HPV subtype infection and cervical intraepithelial neoplasia(CINⅡ) + was analyzed. The women whose pathological diagnosis were CINⅠand cervicitis were followed up for one year by the method of TCT and HPV genotyping detec-tion. Results Of 200 women, 37 patients were CINⅡ+(18. 6%). The risk of patients with HPV16 infection to develop CINⅡ+was 2. 608 times higher than that of patients without HPV16 infection (OR=2. 608, P<0. 05). Of 151

  3. Clinical significance of liquid-based Thinprep Cytology Test and TBS for cervical diseases screening%液基薄层细胞学结合TBS系统对宫颈癌筛查的临床意义

    Institute of Scientific and Technical Information of China (English)

    张蕾; 赵计端

    2013-01-01

    OBJECTIVE To investigate the differences between the Thinprep Cytology Test (TCT) and pathological results, and to evaluate the accuracy of TCT for cervical lesions screening in clinic. METHODS All 800 cases cytologic screening were analyzed retrospectively. 510 cases of 800 cases cytologic screening were received by biopsies. The results of TCT were compared with that of biopsy. RESULTS Among the 800 cases, there were 484 cases (60.5%, 484/800) of NLIM, 316 cases (39.5%, 316/800) of abnormal epithelial cells, which included 8 cases of ASC-US, 32 cases of ASC-H, 15 cases of AGUS, 118 cases of HSIL, 136 cases of LSIL, 5 cases of SCC, 2 cases of AC. The positive rate of lesions up to LSIL was 51.18 % (261/ 510) in TCT and 56.47% (288/510) in biopsy. There was no significant difference between them (x2=2.88, P> 0.05). The coincidence rate of LSIL was 80.88%, HSIL was 93.22%, SCC was 80.0% and AC was 100%. CONCLUSION The liquid -based TCT is a vital method for cervical cancer screening, which can be adopted combined with biopsy to detect precancerous lesions of uterine cervix. TCT is an effective and perfect method for the cervical cancer screening and treatment.%目的 比较宫颈液基薄层细胞学(TCT)与组织病理学检查结果的差异,评价TCT在宫颈病变诊断中的临床价值.方法 回顾性分析800例行TCT的患者,其中有510例行阴道镜下多点活检或宫颈锥切术后行组织病理学检查,对TCT与组织病理学检查结果进行统计学分析.结果 TCT报告NILM 484例(60.5%,484/800);上皮细胞异常316例(39.5%,316/800);其中ASC-US 8例;ASC-H 32例;AGUS 15例;HSIL 118例;LSIL 136例;SCC 5例;AC2例.细胞学诊断LSIL以上病变的阳性率为51.18% (261/510),组织病理学诊断CIN Ⅰ以上病变的阳性率为56.47%(288/510),两者比较差异无统计学意义(x2=2.88,P>0.05).Spearman等级相关分析显示细胞学和组织学检查结果呈高度正相关(r2=0.949,P< 0.05).细胞学诊断

  4. Rescreening in cervical cytology for quality control. When bad data is worse than no data or what works, what doesn't, and why.

    Science.gov (United States)

    Renshaw, Andrew A

    2003-09-01

    The currently mandated methods to measure the sensitivity of Papanicolaou smear interpretation (including the 5-year look back and review of 10% of negative smears) are misleading. They do not allow one to measure the true sensitivity of the test and allow only a small fraction of errors to be detected and corrected. Rapid prescreening and automated screening are the only methods that seem practically feasible, and will allow the sensitivity of the method to be measured on a routine basis, and, thereby allow a reduction in overall errors. Although emerging technologies, such as HPV testing, may allow alternative methods of measuring sensitivity, the most appropriate way to use and interpret these data in this context are not yet fully developed. Unfortunately, at present there seems to be little desire to change the way things are done, and, therefore, to accurately measure sensitivity in cervical cytology. The first task that needs to be undertaken in fixing a problem is to admit that one exists. At present, most laboratory directors believe that their own laboratory is performing satisfactorily. They may well be, although the laboratories lack an analytic method to demonstrate this, and, therefore, the need for better QA methods does not seem to be acute. There is some educational value to the currently practiced and mandated performance measures, the 5-year look back and review of 10% of negative smears. Most laboratory directors seem to be happy with their QA methods and are not concerned that the data that are derived from the 10% review of negative slides does not reflect their actual sensitivity of screening in any meaningful way. Unfortunately, the forces that are currently in place in the United States ensure that accurate measures of the sensitivity of cervical cytology interpretation are unlikely to be implemented beyond the level of individual experimentation. As long as the expectation of cytologists is that the error rates are significantly less than

  5. Association of cervical artery dissection with connective tissue abnormalities in skin and arteries.

    Science.gov (United States)

    Brandt, T; Morcher, M; Hausser, I

    2005-01-01

    Spontaneous cervical artery dissections (sCAD) often occur in otherwise healthy individuals without known risk factors for stroke and frequently develop spontaneously without relevant trauma. An underlying arteriopathy leading to a so-called 'weakness of the vessel wall' and predisposing certain individuals to dissection has often been postulated. Therefore, the morphology of connective tissue, a main component of vessel wall and environment, was investigated in carotids and skin. While the overall morphology of dermal connective tissue is normal, about half of patients with sCAD show mild ultrastructural connective tissue alterations. These ultrastructural morphological aberrations can be designated either as 'Ehlers-Danlos syndrome (EDS) III-like', resembling mild findings in patients with the hypermobility type of EDS (EDS III); or coined 'EDS IV-like' with collagen fibers containing fibrils with highly variable diameters resembling mild findings in vascular EDS; or the abnormalities are restricted to the elastic fibers (with fragmentation and minicalcifications) without significant alterations in the morphology of the collagen fibrils. These findings had some similarity with the morphology found in heterozygous carriers of pseudoxanthoma elasticum. A grading scale according to the severity of the findings has been introduced. Similar connective tissue abnormalities were detected in some first-degree relatives of patients with sCAD showing hereditary at least in a subgroup. They can serve as a phenotypic marker for further genetic studies in patients with sCAD and large families to possibly identify the underlying basic molecular defect(s). Very few of patients (connective tissue abnormalities have clinical manifestations of skin, joint, or skeletal abnormalities of a defined heritable connective tissue disorder. In specimens of arterial walls of carotid, aortic, and renal arteries of patients with sCAD, pronounced systemic, histopathological, and

  6. Inter-Rater Agreement of Anal Cytology

    OpenAIRE

    Darragh, Teresa M.; Tokugawa, Diane; Castle, Philip E.; Follansbee, Stephen; Borgonovo, Sylvia; LaMere, Brandon J.; Schwartz, Lauren; Gage, Julia C.; Fetterman, Barbara; Lorey, Thomas; Wentzensen, Nicolas

    2012-01-01

    Most anal cancers are caused by persistent infections with carcinogenic human papillomaviruses (HPV). Similar to cervical carcinogenesis, the progression from HPV infection to anal cancer goes through precancerous lesions that can be treated to prevent invasion. In analogy to cervical cytology, anal cytology has been proposed as a screening tool for anal cancer precursors in high-risk populations. We analyzed the inter-observer reproducibility of anal cytology in a population of 363 HIV-infec...

  7. Diagnosis value of liquid-based cytology and conventional cytology smears on detecting cervical squamous cell lesions%液基细胞学技术与传统细胞学涂片技术对宫颈病变细胞学的诊断价值

    Institute of Scientific and Technical Information of China (English)

    董彦军; 郑加荣

    2013-01-01

    Objective To compare the value of liquid-based cytology and conventional cytology smears on detection of cervical squamous cell lesions.Methods One thousand and two hundred women were detected by cervical liquid-based cytology and conventional cytology smear screening from Oct.2009 to Oct.2012,of which positive cases were detected by colposcopy and biopsy as the gold standard as a positive control.The diagnostic value of the liquid-based cytology on cervical lesions was assessed.Results The positive detection rate of ASCUS/A-GUS and above lesions was 16.00% (96 cases) by liquid-based cytology detection,and positive detection rate of pap ⅡB level and above lesions was 4.83 % (29 cases).The positive detection rate by liquid-based cytology was significantly higher than that of conventional cytology smears,the difference was statistically significant (P < 0.05).Colposcopic and histopathological findings as the gold standard,cervical liquid-based cytology screening coincidence rate was 76.80%,conventional cytology smear screening coincidence rate was 38.16%,and the difference was statistically significant (P < 0.05).Conclusions Liquid-based cytology screening for cervical squamous cell lesions is significantly superior to conventional cytology smears.%目的 比较液基细胞学技术与传统细胞学涂片在宫颈鳞状细胞病变检测中的价值.方法 对2009年10月至2012年10月1200例妇女采用宫颈液基细胞学或传统细胞学涂片进行筛查,并对筛查阳性病例以其阴道镜检查及病理活检结果为金标准作为阳性对照,评估液基细胞学技术对宫颈不同病变的诊断价值.结果 宫颈液基细胞学筛查出ASCUS/A-GUS及以上分级病例96例,阳性检出率为16.00%;传统细胞学涂片筛查出巴氏ⅡB级及以上分级病例29例,阳性检出率为4.83%;宫颈液基细胞学阳性检出率明显高于传统细胞学涂片,差异有统计学意义(P<0.05).以阴道镜及病理组织学

  8. 液基细胞结合阴道镜下活钳在宫颈病变中的应用%Application of Liquid Based Cytology Combined with Colposcopy Biopsy in Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    税锦华; 李晓冬

    2014-01-01

    目的:了解液基细胞结合阴道镜下活钳对诊断宫颈病变的临床价值。方法对我院2010年1月1日至2013年12月31日妇科门诊自愿作TCT的6718例已婚妇女作TCT检测,结果异常的再作阴道镜活钳。TCT检查:用特制的塑料刷在宫颈内口磷柱状上皮交界处旋转6~8周,放入液基细胞保存液瓶中,经自动制片机制成簿片,经巴氏染色封片,由专业病检医生读片。阴道镜检查由专业从事阴道镜的医生,在子宫颈转化区3、6、9、12点处活钳或采用醋酸涂宫颈在醋酸白上皮区取材行宫颈活钳。结果⑴TCT筛查6718例异常的573例,占8.57%。⑵阴道镜下活钳573例病理结果:炎症201例,CINⅠ287例,CINIⅡ53例,CINⅢ24例,SCC8例。结论液基细胞学结合阴道镜活检对宫颈病变可提高阳性诊断率,做到早期诊断、早期治疗。%Objective To understand the clinical value of the liquid based cytology combined with colposcopy biopsy in the diagnosis of cervical lesions.Methods 6718 married women who were voluntary to test by TCT were selected by the out-patient gynecological department of our hospital from January 1, 2010 to December 31, 2013, while the abnormal one was tested by further colposcopy biopsy. TCT examination: the special plastic brush was used to rotate 6 - 8 weeks in the junction of endocervix phosphorus columnar epithelial cell, then the cells were put into basal cell preservation liquid bottles. The slices were made by automatic production machine and stained by Pap stain and mounting. The pathological specimens were checked by professional doctor in under the microscope. Colposcopy operation was performed by specializing colposcopy doctors in 3, 6, 9, 12 at the cervical transformation zone given or smearing acetate on cervix then given cervicalbiopsy on acetowhite epithelium area.Results (1) The screening of TCT in 6718 cases of abnormal 573, accounted for 8.57%.(2) The results of 573

  9. Intra- and inter-observer reliability of MRI examination of intervertebral disc abnormalities in patients with cervical myelopathy

    Energy Technology Data Exchange (ETDEWEB)

    Braga-Baiak, Andresa [Center for Excellence in Surgical Outcomes, Duke University Medical Center, Durham, NC (United States); Post-graduation Program, Department of Radiology, University of Sao Paulo (Brazil); Shah, Anand [Center for Excellence in Surgical Outcomes, Duke University Medical Center, Durham, NC (United States); Pietrobon, Ricardo [Center for Excellence in Surgical Outcomes, Duke University Medical Center, Durham, NC (United States); Department of Surgery, Duke University Medical Center, Durham, NC (United States); Braga, Larissa [Center for Excellence in Surgical Outcomes, Duke University Medical Center, Durham, NC (United States); University of Nebraska Medical Center, Lincoln NE (United States); Neto, Arnolfo Carvalho [Clinica DAPI, Curitiba (Brazil); Section of Diagnostic Radiology, Department of Internal Medicine, Universidade Federal do Parana (Brazil); Cook, Chad [Center for Excellence in Surgical Outcomes, Duke University Medical Center, Durham, NC (United States); Division of Physical Therapy, Duke University Medical Center, Durham, NC (United States)], E-mail: chad.cook@duke.edu

    2008-01-15

    Purpose: Intervertebral cervical disc herniation (CDH) is a relatively common disorder that can coexist with degenerative changes to worsen cervicogenic myelopathy. Despite the frequent disc abnormalities found in asymptomatic populations, magnetic resonance imaging (MRI) is considered excellent at detecting cervical spine myelopathy (CSM) associated with disc abnormality. The objective of this study was to investigate the intra- and inter-observer reliability of MRI detection of CSM in subjects who also had co-existing intervertebral disc abnormalities. Materials and methods: Seven experienced radiologists reviewed twice the MRI of 10 patients with clinically and/or imaging determined myelopathy. MRI assessment was performed individually, with and without operational guidelines. A Fleiss Kappa statistic was used to evaluate the intra- and inter-observer agreement. Results: The study found high intra-observer percent agreement but relatively low Kappa values on selected variables. Inter-observer reliability was also low and neither observation was improved with operational guidelines. We believe that those low values may be associated with the base rate problem of Kappa. Conclusion: In conclusion, this study demonstrated high intra-observer percent agreement in MR examination for intervertebral disc abnormalities in patients with underlying cervical myelopathy, but differing levels of intra- and inter-observer Kappa agreement among seven radiologists.

  10. Cervical lymph node metastases from thyroid cancer: does thyroglobulin and calcitonin measurement in fine needle aspirates improve the diagnostic value of cytology?

    Directory of Open Access Journals (Sweden)

    Baldini Enke

    2013-02-01

    Full Text Available Abstract Background Measurement of thyroglobulin (Tg protein in the washout of the needle used for fine needle aspiration biopsy cytology (FNAB-C has been shown to increase the sensitivity of FNAB-C in identifying cervical lymph node (CLN metastasis from well-differentiated thyroid cancer (TC. In this study, we evaluated whether routine measurement of Tg protein (FNAB-Tgp, Tg mRNA (FNAB-Tgm and calcitonin (CT mRNA (FNAB-CTm in the FNAB washout of CLN increases the accuracy of FNAB-C in the diagnosis of suspicious metastatic CLN. Methods In this prospective study 35 CLN from 28 patients were examined. Histology showed metastatic papillary TC (PTC in 26 CLN, metastatic medullary TC (MTC in 3 CLN, metastatic anaplastic TC (ATC in 3 CLN and 3 metastatic CLN from extra-thyroidal cancers. Results The overall accuracy of FNAB-C was 84.4%, reaching 95.7% when the analysis was restricted to PTC. Both FNAB-Tgp and FNAB-Tgm compared favorably with FNAB-C and shown diagnostic performances not statistically different from that of FNAB-C. However, FNAB-Tgp and FNAB-Tgm/FNAB-CTm were found useful in cases in which cytology results were inadequate or provided diagnosis inconsistent with patient's clinical parameters. Conclusions We demonstrated that FNAB-C, Tg/CT mRNA and Tg protein determination in the fine-needle washout showed similar accuracy in the diagnosis of metastatic CLN from TC. The results of this study suggest that samples for Tg protein and Tg/CT mRNA measurements from CLN suspicious for metastatic TC should be collected, but their measurements should be restricted to cases in which FNAB-C provides uninformative or inconsistent diagnosis with respect to patient's clinical parameters.

  11. Correlation of plasma nitrite/nitrate levels and inducible nitric oxide gene expression among women with cervical abnormalities and cancer.

    Science.gov (United States)

    Sowjanya, A Pavani; Rao, Meera; Vedantham, Haripriya; Kalpana, Basany; Poli, Usha Rani; Marks, Morgan A; Sujatha, M

    2016-01-30

    Cervical cancer is caused by infection with high risk human papillomavirus (HR-HPV). Inducible nitric oxide synthase (iNOS), a soluble factor involved in chronic inflammation, may modulate cervical cancer risk among HPV infected women. The aim of the study was to measure and correlate plasma nitrite/nitrate levels with tissue specific expression of iNOS mRNA among women with different grades of cervical lesions and cervical cancer. Tissue biopsy and plasma specimens were collected from 120 women with cervical neoplasia or cancer (ASCUS, LSIL, HSIL and invasive cancer) and 35 women without cervical abnormalities. Inducible nitric oxide synthase (iNOS) mRNA from biopsy and plasma nitrite/nitrate levels of the same study subjects were measured. Single nucleotide polymorphism (SNP) analysis was performed on the promoter region and Ser608Leu (rs2297518) in exon 16 of the iNOS gene. Differences in iNOS gene expression and plasma nitrite/nitrate levels were compared across disease stage using linear and logistic regression analysis. Compared to normal controls, women diagnosed with HSIL or invasive cancer had a significantly higher concentration of plasma nitrite/nitrate and a higher median fold-change in iNOS mRNA gene expression. Genotyping of the promoter region showed three different variations: A pentanucleotide repeat (CCTTT) n, -1026T > G (rs2779249) and a novel variant -1153T > A. These variants were associated with increased levels of plasma nitrite/nitrate across all disease stages. The higher expression of iNOS mRNA and plasma nitrite/nitrate among women with pre-cancerous lesions suggests a role for nitric oxide in the natural history of cervical cancer. PMID:26435258

  12. 液基细胞学技术与传统细胞学涂片检测宫颈鳞状细胞病变的比较%Comparison of liquid - based cytological test and routine cytological smears in detection of cervical squamous cell lesions

    Institute of Scientific and Technical Information of China (English)

    印永祥; 赵华; 黄望珍

    2012-01-01

    目的:评价液基细胞学技术与传统细胞学涂片检测宫颈鳞状细胞病变的差异.方法:比较同地区、同期宫颈液基细胞学筛查病例及传统细胞学涂片筛查病例,同时收集液基细胞组中289例阴道镜检查及病理活检结果及传统细胞组中62例阴道镜检查及病理活检结果进行分析,评估不同病变的假阳性率.结果:液基细胞组标本不满意率(2.71%)明显低于传统细胞组标本(3.96%)(P<0.01).液基细胞组对不明意义的非典型鳞状细胞(ASC - US)及更高级别的病变检出率(2.25%)明显高于传统细胞组(0.74%)(P<0.01).液基细胞组ASC - US占低度鳞状上皮内病变(LSIL)、非典型鳞状细胞不除外高度病变( ASC - H)、高度鳞状上皮内病变(HSIL)及鳞癌(SCC)总数的比例(0.82%)明显低于传统细胞组(2.11%)(P<0.01).传统细胞组与液基细胞组假阳性率比较,差异有统计学意义(P<0.01),传统细胞组ASC -US、ASC -H和SCC中假阳性率与液基细胞组比较差异无统计学意义.组织学活检与细胞学结果比较,年龄≥35岁液基细胞组ASC - US阳性预测值与传统细胞组比较差异无统计学意义(P>0.01);年龄<35岁液基细胞组ASC - US阳性预测值明显高于传统细胞组(P<0.01).结论:液基细胞学技术检测宫颈鳞状细胞病变明显优于传统细胞学涂片,高质量的宫颈细胞标本对提高诊断的准确性非常重要;选择不同方法筛查宫颈病变时,目标人群的年龄是一个重要的决定因素.%Objective; To evaluate the difference of liquid - based cytological technique and routine cytological smears in detection of cervical squamous cell lesions. Methods; The cases receiving cervical liquid - based cytological screening and the cases receiving routine cytological smears screening in the same region and during the same period were compared. The results of colposcopy and pathological biopsy of 289 cases from liquid - based

  13. 探讨毛细式液基细胞学薄层染色技术对宫颈癌筛查应用价值研究%Sutdy on the Application Value of the Capillary Liquid- based Cytology Thin Layer Staining Technique (TCT) in the Screening of Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    刘新梅

    2016-01-01

    Objective To discuss the clinical application value of the capillary liquid- based cytology thin layer staining technique (TCT) in the screening of cervical cancer. Methods 480 cases of patients undergoing cervical cancer screening in our hospital from June 2011 to July 2013 were randomly selected and were given TCT examination, the equivocal atypical squamous epithelial cells (ASCUS) or above were regarded as the positive lesions and they were given the biopsy by col-poscopy, and the biopsy results and cytological test results were compared and analyzed. Results There were 102 positive specimens of the 480 cases of patients, and the positive rate was 21.25%; the coincidence rate of TCT examination and biopsy by colposcopy of the 35 cases abnormal was 88.57%. Conclusion Capillary liquid-based cytology thin layer staining technique (TCT) in the screening of cervical cancer can effectively improve the screening quality, which is of important sig-nificance to the prevention and treatment of cervical cancer.%目的 探讨毛细式液基细胞学薄层染色技术(TCT)对宫颈癌筛查的临床应用价值. 方法 随机选取2011年6月—2013年7月期间在该院行宫颈癌筛查的患者480例,所有患者均行TCT检查,将其中意义不明确的不典型鳞状上皮细胞(ASCUS)以上病变视为阳性病变,并对其进行阴道镜活检,将活检结果和细胞学检测结果进行对比分析.结果 480例患者中,共计得到102例阳性标本,阳性率为21.25%;其中35例异常者的TCT检查的同阴道镜活检的符合率为88.57%. 结论 在宫颈癌筛查中应用毛细式液基细胞学薄层染色检查,可有效提高筛查质量,对于宫颈癌的预防和治疗具有十分重要的意义.

  14. Desempenho do exame colpocitológico com revisão por diferentes observadores e da captura híbrida II no diagnóstico da neoplasia intra-epitelial cervical graus 2 e 3 Performance of cervical cytology with review by different observers and hybrid capture II in the diagnosis of cervical intraepithelial neoplasia grades 2 and 3

    Directory of Open Access Journals (Sweden)

    André Luís Ferreira Santos

    2003-08-01

    Full Text Available Para avaliar o desempenho da colpocitologia (CO de encaminhamento e daquela coletada no serviço de referência, com revisão por diferentes técnicas e observadores, e da captura híbrida II (CH II no diagnóstico da neoplasia intra-epitelial cervical (NIC de alto grau, foram incluídas 105 mulheres atendidas entre agosto de 2000 e junho de 2001 por atipias pré-neoplásicas na CO. Todas foram submetidas à coleta de nova CO e CH II para detecção do DNA-HPV. Foi realizada biópsia cervical em 91, sendo o diagnóstico histológico considerado padrão ouro. Foram descritas a sensibilidade, especificidade e razão de verossimilhança positiva (RVP dos métodos propedêuticos com intervalo de confiança de 95% (IC95%. A sensibilidade e especificidade da CO de encaminhamento foi de 57% e 82% para o diagnóstico de NIC 2 e 3, e a RVP de 3,2 (IC95%: 1,5-6,8. A CO do serviço mostrou uma sensibilidade e especificidade 79% e 84%, respectivamente e RVP de 5,0 (IC95%: 2,5-10,0. A sensibilidade (86%, especificidade (80% e RVP (4,3 foram semelhantes com a revisão lenta realizada pelo segundo observador, havendo uma queda significativa da sensibilidade (64% à revisão rápida pelo terceiro observador. A CH II mostrou alta sensibilidade (100%, baixa especificidade (43% e baixa RVP (1,7, IC95%: 1,4-2,2.To evaluate the performance of initial cervical cytology and that collected at the reference service with a review conducted by different observers and techniques, as well as hybrid capture II, in the diagnosis of high-grade cervical intraepithelial neoplasia (CIN, 105 women attended from August 2000 to June 2001 for preneoplastic atypia upon cervical cytology were included. A new cervical cytology and hybrid capture II for DNA-HPV were conducted in all the patients. Cervical biopsies were taken in 91 women. Performance of the investigative procedures was described by estimating the sensitivity, specificity, and positive likelihood ratio (PLR, with a 95

  15. Cervical Liquid-based Cytology in Cervical Lesions the Pathological Diagnosis of Significance%宫颈液基薄层细胞学在宫颈病变病理诊断中的意义

    Institute of Scientific and Technical Information of China (English)

    郑国文

    2013-01-01

      Objective To investigate the liquid-based cytology (TCT) value in pathological diagnosis of cervical lesions. Methods Retrospective analysis of 2009 January to 2012 June, 5795 patients with TCT cervical cytology, colposcopy biopsy positive cases, compared the two results. Results In 5499 cases of TCT were negative, 296 cases were positive, the positive rate was 5.11%, false negative in 15 cases, no false positive cases. 17 cases of biopsy tissue inflammation, 224 cases of CIN Ⅰ, 20 cases of CINⅡ, 30 cases of CINⅢ, CA 5 cases, LSIL and CIN Ⅰcoincidence rate was 80% (36/45), HSIL and CINⅡ/Ⅲcoincidence rate was 88.9% (24/27). Conclusion TCT examination has a little injury, high positive rate, strong repeatability, for married women can be used as the main method of screening for cervical cancer and precancerous lesions.%  目的探讨液基薄层细胞学(TCT)检查在宫颈病变病理诊断中的意义。方法回顾分析我院2009年1月至2012年6月5795例患者运用 TCT 进行宫颈脱落细胞学检查,阳性病例在阴道镜下活检,二者结果相比较分析。结果 TCT 检查阴性5499例,阳性296例,阳性率5.11%,假阴性15例,无假阳性病例。组织活检炎症17例,CIN Ⅰ224例,CIN Ⅱ20例,CIN Ⅲ30例,CA 5例,LSIL 与 CIN Ⅰ符合率80.0%(36/45),HSIL 与 CIN Ⅱ/Ⅲ符合率88.9%(24/27)。结论 TCT 检查具有损伤小,阳性检出率高,重复性强,对已婚妇女可作为宫颈癌及癌前病变筛查的主要方法。

  16. 液基薄层细胞学检测对宫颈病变的诊断价值%Diagnostic value of thinPrep liquid-base cytologic test(TCT)examination in patients with cervical disease

    Institute of Scientific and Technical Information of China (English)

    朱淑玲; 武彤彤

    2010-01-01

    目的 探讨液基薄层细胞学检测(TCT)技术对宫颈癌前病变的诊断价值.方法 收集宫颈病变患者1 000例,分别应用TCT(500例)检查及传统巴氏涂片(500例)检查.结果 TCT法满意490例(98.0%),高于传统涂片法的440例(88.0%)(χ~2=3.987,P<0.05);传统涂片法检查ⅡB~V级阳性50例(10.0%)低于TCT法的85例(17.1%)(χ~2=3.923,P<0.05);TCT检查与病理活检的阳性符合率64.7%,明显高于传统涂片法的44.0%(χ~2=3.893,P<0.05).结论 TCT技术明显提高了标本的满意率及宫颈异常细胞检出率,并且与组织病理活检有较高的阳性符合率,适合于临床推广应用.%Objective To evaluate the clinical significance of liquid-based cytological test(TCT)examina-tion for screening of cervical disease.Method 1000 case were divided into two groups,500 cases were examined by liquid-based cytological examination.and 500 cases were examined by traditional Papanicoloaou smear.Results TCT satisfied with 490 cases(98.0%)which was higher than the traditional smear method 440 cases(88.0%)(χ~2=3.987,P<0.05);The traditional smear examination ⅡB~V-class positive 50 cases(10.0%)were lower than TGT 85 cases(17.1%)(χ~2=3.923,P<0.05);TCT examination、with biopsy of the positive coincidence rate of 64.7% were significantly higher than the traditional smear 44.0%(χ~2=3.893,P<0.05).Conclusion TCT technology significantly improves satisfaction rates and detection rate of abnormal cells,also with the pathology biopsy have a higher positive coincidence rate,suitable for clinical application.

  17. The possible role of cranio-cervical trauma and abnormal CSF hydrodynamics in the genesis of multiple sclerosis.

    Science.gov (United States)

    Damadian, Raymond V; Chu, David

    2011-01-01

    UPRIGHT Multi-Position MR scanning has uncovered a key set of new observations regarding Multiple Sclerosis (MS), which observations are likely to provide a new understanding of the origin of MS. The new findings may also lead to new forms of treatment for MS. The UPRIGHT MRI has demonstrated pronounced anatomic pathology of the cervical spine in five of the MS patients studied and definitive cervical pathology in the other three. The pathology was the result of prior head and neck trauma. All eight MS patients entered the study on a first come first serve basis without priority, and all but one were found to have a history of serious prior cervical trauma which resulted in significant cervical pathology. The cervical pathology was visualized by UPRIGHT MRI. Upright cerebrospinal fluid (CSF) cinematography and quantitative measurements of CSF velocity, CSF flow and CSF pressure gradients in the upright patient revealed that significant obstructions to CSF flow were present in all MS patients. The obstructions are believed to be responsible for CSF "leakages" of CSF from the ventricles into the surrounding brain parenchyma which "leakages" can be the source of the MS lesions in the brain that give rise to MS symptomatology. The CSF flow obstructions are believed to result in increases in intracranial pressure (ICP) that generate "leakages" of the CSF into the surrounding brain parenchyma. In all but one MS patient, anatomic pathologies were found to be more severe in the upright position than in the recumbent position. Similarly, CSF flow abnormalities were found to be more severe in the upright position than in the recumbent position in all but one MS patient. Images of the MS patient anatomic pathologies and CSF flow abnormalities are provided with comparison images from normal examinees in Figures 1-15. PMID:21970155

  18. Minor Cytological Abnormalities and up to 7-Year Risk for Subsequent High-Grade Lesions by HPV Type.

    Directory of Open Access Journals (Sweden)

    Maria Persson

    Full Text Available Diagnoses of atypical squamous cells of undetermined significance (ASCUS and low-grade squamous intraepithelial lesions (LSIL are common, but the corresponding risk of disease varies by human papillomavirus (HPV status, complicating management strategies. Our aim was to estimate the longer-term risk of cervical intraepithelial neoplasia grade 2 or worse (CIN2+ among women with ASCUS/LSIL by age, HPV status, and genotype(s.A total of 314 women with ASCUS/ LSIL were followed for a median of 3.8 years. Baseline HPV status was determined by reflex testing and women with histologically confirmed CIN2+ were identified through linkage to the Swedish National Quality Register for Cervical Cancer Prevention. Cumulative incidence and hazard ratios were estimated to explore differences between index data and associations with CIN2+.In total, 89 women (28.3% developed CIN2+. High-risk (HR HPV-positive women developed significantly more CIN2+ than HR-HPV-negative women (cumulative incidence 3.5 years after the index test: 42.2%, 95% CI: 32.5-53.5 for HPV16/18; 36.2%, 95% CI: 28.3-45.4 for other HR-HPV types; and 2.0%, 95% CI: 0.5-7.8 for HR-HPV-negative women; p<0.0001.HPV status was of greatest importance in determining the risk of CIN2+. The risk was low among HPV-negative women during the first years of follow-up, suggesting these women could be followed less intensively. HPV16/18-positive women may need intensified follow-up as they showed the highest risk of CIN2+.

  19. Clinical comparison between traditional Bethesda smears and liquid-based cytology test in the screening of cervical cancer%两种宫颈癌筛查方法对比研究及临床分析

    Institute of Scientific and Technical Information of China (English)

    李新敏; 古雅丽

    2012-01-01

    目的:探讨传统巴氏涂片法和液基细胞学法在宫颈癌筛查中的临床意义.方法:分别对12302例和15750例患者分别进行传统巴氏涂片法和液基细胞学检测的宫颈癌筛查.对细胞学阳性者进行阴道镜下活检并分析结果.结果:传统巴氏涂片法和液基细胞学检测的阳性检出率分别为5.45%和6.06%,差异无统计学意义(P>0.05);两种方法与活检符合率分别为22.84%和55.57%,差异有统计学意义(P<0.05);两种方法的假阳性率分别为77.16%和44.42%,差异有统计学意义(P<0.05).结论:液基细胞学检测用于宫颈癌的筛查与活检的符合率高,假阳性率低,优于传统巴氏涂片,值得在临床推广应用.%Objective: To explore clinical value of traditional Bethesda smears and liquid - based cytology test in the screening of cervical cancer. Methods: Traditional Bethesda smears and liquid-based cytology test were used to screen 12302 and 15750 subjects with cervical cancer, respectively. Biopsy under the colposcope was performed for the subjects with the positive outcome of cytological examination. Results: The positive rates identified by traditional Bethesda smears and liquid-based cytology test were 5.45% and 6.06%, respectively (P>0.05). The coincidence positive rate for traditional Bethesda smear and biopsy under the colposcope was 22.84%. The coincidence positive rate for liquid-based cytology test and biopsy under the colposcope was 55.57% (P<0.05). The fake positive rates for traditional Bethesda smear and liquid-based cytology test were 77.16% and 44.42%, respectively (P<0.05). Conclusion: Liquid-based cytology test is worth to be recommended in the clinical examination of cervical cancer due to its higher coincidence rate with pathological examination and its lower fake-positive rate.

  20. Coverage of cervical cancer screening in Catalonia for the period 2008-2011 among immigrants and Spanish-born women.

    Directory of Open Access Journals (Sweden)

    Vanesa eRodriguez-Sales

    2013-12-01

    Full Text Available Background: The Public Health system in Catalonia, Spain provides a free of charge opportunistic cervical cancer screening. In June 2006 a revised recommendations for cervical cancer screening was introduced to increase coverage and to promote a three-year interval between screening tests. Aim: This study examines cervical cancer screening coverage and prevalence of cytology abnormalities in Catalonia by immigration status.Methods: The study analyses the cytologies registered among women aged 25-65 that have been attended at the Primary Health Centers (PHC for any reason (n=1,242,105 during 2008-11. Coverage was estimated from Governmental data base Information System Primary Care (SISAP that includes 77% of PHC. The database is anonymous, and includes information on age, country of birth, diagnostic center and cytology results Results: During the period 2008-2011, 758,690 smears were performed in a total of 595,868 women over 14 years, of whom 18.3% were immigrants. Cytology coverage was higher among immigrant women compared to Spanish born (51.2% and 39% respectively. Immigrant women also had a higher prevalence of abnormal Paps compared to the Spanish population, 4.9% and 3.3% respectively.Conclusion: Immigrant women in Catalonia have a good access to the Public Health Services and to cervical cancer screening facilities. The higher prevalence of abnormal cytologies in immigrant women compared to native women indicates the relevance to prioritize cervical cancer screening activities on a regular base in new comers.

  1. Comprehensive evaluation of early screening of cervical cancer cytological examination%宫颈癌早期筛查细胞学检查方法综合评价

    Institute of Scientific and Technical Information of China (English)

    刘玉瑰

    2015-01-01

    Objective To appraise the comprehensive evaluation of early screening of cervical cancer cytological examination.Methods From October 2012 to October 2014, 1000 cases of gynecology clinic admissions were selected, and they were randomly divided into group A and group B, with 500 cases in each group, group A was given Pap cytology, while B group was given ThinPrep cytology (TCT), comparative analysis of test results, while the colposcopic biopsy histology results were analyzed.Results CIN and cervical cancer diagnostic sensitivity and specificity was significantly higher in group B than those in group A (P < 0.05).Conclusions Early cervical cancer screening to take ThinPrep cytology diagnosis can be achieved relatively good sensitivity and specificity, it is worth learning from.%目的 综合评价宫颈癌早期筛查细胞学检查方法.方法 抽取2012年10月至2014年10月妇科门诊接诊的患者1000例,随机分为A组与B组,每组500例,其中A组采取巴氏细胞学检查,而B组采取液基薄层细胞学检查(TCT),对比分析检查结果,同时与阴道镜组织学活检结果进行对比分析.结果 B组诊断CIN及宫颈癌的敏感性与特异性皆高于A组(P<0.05).结论 宫颈癌早期筛查采取液基薄层细胞学检查诊断可取得比较好的敏感性与特异性,值得借鉴.

  2. Expression of cellular bromodomain protein 4 and human papillomavirus-16 E2 protein in cervical cytological specimens%Brd4与HPV-16病毒E2在宫颈脱落细胞中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    黄卓敏; 江曼茹; 王楚莹; 姚吉龙; 谢建生

    2012-01-01

    Objective To investigate the expression of cellular bromodomain protein (Brd4) and human papillomavirus-16 (HPV-16) E2 protein in cervical cytological specimens as well as their potential roles in cervical carcinogenesis and their potential clinical significances. Methods The expression of Brd4 and E2 protein in 150 cases of cervical cytological specimens with HPV-16 infection was detected by Western-blot assay. According to cytological diagnosis, the samples were classified into five groups, including 30 cases of normal control, 46 cases of ASCUS, 23 cases of LSIL, 31 cases of HSIL and 20 cases of cervical cancer. Then, 120 cases with abnormal cytology were divided into four groups by histopathological identification, including 21 cases of cervicitis, 25 cases of CIN I , 45 cases of CIN Ⅱ/Ⅲ and 29 cases of invasive cervical carcinoma as well. Results Brd4 and E2 protein expression significantly decreased in groups of ASCUS, LSIL, HSIL and cervical carcinoma compared with that in normal cervical exfoliated cells. Meanwhile, Brd4 and E2 protein expression in groups of HSIL and cervical carcinoma was significantly lower than that of LSIL and HSIL. No significant difference of Brd4 and E2 protein expression was found in both groups of cervicitis and CIN I (P > 0.05). However, Brd4 and E2 protein expression was decreased dramatically corresponding with pathological upgrading from CIN group to invasive cervical carcinoma group(P < 0.05). The sensitivity, specificity, positive predictive value and negative predictive value of Brd4 and E2 protein for screening potential cervical lesions in ASCUS was 0.93, 0.94, 0.88, 0.98 and 0.80, 0.95, 0.86, 0.93, respectively. Conclusions Deletion of HPV-16 E2 protein induced by degradation of Brd4-E2 complex has been proved to be a key step in the malignant transformation of HPV-induced cervical cells, which may play an important role in cervical carcinogenesis. Therefore, Brd4 and E2 protein expression may be used for

  3. 颈椎生理曲度异常与颈椎病发病关系分析%Relationship analysis of abnormal physiological curvature of cervical vertebrae and cervical spondylosis

    Institute of Scientific and Technical Information of China (English)

    王黎明; 吕宏乐; 关海森; 贾艳领; 韩莉娟

    2015-01-01

    ObjectiveTo study relationship of abnormal physiological curvature of cervical vertebrae and cervical spondylosis.Methods 200 patients with cervical spondylosis and 200 patients without cervical spondylosis were randomly selected from May 2013 to June 2013 in the hospital, they were allocated to the observation group and the control group. Lateral positions of cervical vertebrae of patients in two groups were detected by X-ray. Shapes of curvature of cervical vertebrae of two groups were compared and the difference of probability of abnormal physiological curvature of cervical vertebrae of two groups was observed.Results In the observation group, there were 179 patients with abnormal curvature of cervical vertebrae accounting for 89.5% and 21 patients with normal curvature of cervical vertebrae accounting for 11.5%. In the control group, there were 69 patients with abnormal curvature of cervical vertebrae accounting for 34.5% and 131 patients with normal curvature of cervical vertebrae accounting for 65.5%. Conclusion Abnormal curvature of cervical vertebra is common in patients with cervical spondylosis, which is very meaningful for clinical treatment of cervical spondylosis.%目的:研究颈椎生理曲度异常与颈椎病发病之间的关系。方法选择2013年5~6月医院颈椎病病患200例和无颈椎病患者200例,分为观察组和参照组。参照组与观察组均进行颈椎侧位的X线摄片,对比研究两组人员颈椎曲度的形态,观察颈椎生理曲度发生异常的概率的差异性。结果观察组出现颈曲异常的有179例,占总人数的89.5%,颈曲正常的有21例,占总人数的11.5%;无颈椎病人员颈曲异常的有69例,占总人数的34.5%,颈曲正常的有131例,占总人数的65.5%。结论颈椎曲度异常在颈椎病患者中是普遍存在的,这对颈椎病的临床医治是很有意义的。

  4. Cervical cytology screening among low-income, minority adolescents in New York City following the 2009 ACOG guidelines

    Science.gov (United States)

    Tsui, Jennifer; Hofstetter, Annika M.; Soren, Karen

    2014-01-01

    Objectives In December 2009, the American College of Obstetricians and Gynecologists recommended cervical cancer screening begin at age 21 for young women. In this study, we examine receipt of first lifetime Papanicolaou (Pap) test and predictors of over-screening among adolescents within a large urban ambulatory care network. Methods We compared the proportion of first lifetime Pap test of adolescents aged 13-20 years between June 2007-November 2009 (n=7700) and December 2009-June 2012 (n=9637) using electronic health records. We employed multivariable regression models to identify demographic and health care factors associated with receiving a first lifetime Pap test at age <21 years in the post-guideline period (over-screening). Results The proportion of Pap tests declined from 19.3% to 4.2% (p <0.001) between the two periods. Multivariable logistic regression results showed receiving care from gynecology/obstetrics/family planning clinics compared to pediatric clinics, having more clinic encounters, and older age were associated with over-screening in the post-guideline period. Conclusions We found that guideline adherence differed by clinic type, insurance status, and health care encounters. In the quickly evolving field of cervical cancer control, it is important to monitor practice trends as they relate to shifts in population-based guidelines, especially in high-risk populations. PMID:24650625

  5. Discussion of Results Inconsistent between the Cervical Biopsy and Cytological Smear%宫颈活检病理诊断与细胞学涂片结果不符的探讨

    Institute of Scientific and Technical Information of China (English)

    贠世娟; 赵素香; 徐辉

    2014-01-01

    Objective:To improve the cytological diagnosis level of cervical lesions and investigate the situation adapt to cervical biopsy.Method:31 pathological sections and cytological smears which results inconsistent between the cervical biopsy and cytological smear in our hospital from 2008 to 2012 were reexamined.Result:Biopsy pathological diagnosis:1 case of AIS;3 cases of CIN1;1 case of CIN hard classification;6 cases of AIM;2 cases of HPV infection;6 cases of ISM;2 cases of atrophy;10 cases of chronic cervicitis.Conclusion:The inspection results of cervical lesions are directly related to the accuracy of cytologic diagnosis which depends on diagnostician's cognitive level.If the cytologic interpretation results are cancer or HSIL,the direct cervical biopsy should be done.If the cytologic interpretation results are LSIL,ASC-H,ASCUS, the vaginoscopy should be done first,then determine whether it’s worth doing cervical biopsy,AGC,cervical canal biopsy or endometrium biopsy related to tumor.%目的:提高对宫颈病变的细胞学诊断水平及探讨适应宫颈活检的情况。方法:复查本院2008-2012年宫颈活检病理诊断与细胞学涂片结果不符31例病理切片及细胞学涂片。结果:活检病理诊断:原位腺癌1例;CIN1(上皮内肿瘤1级)3例;CIN难分级1例;AIM(不典型不成熟鳞状细胞化生)6例;HPV(人乳头状瘤病毒)感染2例;ISM(不成熟鳞状细胞化生)6例;萎缩2例;慢性宫颈炎10例。结论:细胞学诊断的准确性直接关系到宫颈病变的检出,而其依赖于诊断医生的认识水平;细胞学判读结果为癌或HSIL(高级别鳞状上皮内病变)时直接做宫颈活检,为LSIL(低级别鳞状上皮内病变)、ASC-H(非典型性鳞状细胞、不除外HSIL)、ASC-US(意义不明确的非典型性鳞状细胞)时至少要做阴道镜检查后,再决定是否做宫颈活检,AGC(非典型性腺细胞)倾向于肿瘤的

  6. 液基细胞学和巴氏涂片在宫颈癌筛查中的对比研究%Comparative study of liquid-based cytology and Pap smear in the screening of cervical cancer

    Institute of Scientific and Technical Information of China (English)

    赵章树

    2013-01-01

    Objective: To explore the value of liquid-based cytology and Pap smear in cervical cancer screening of the general population. Methods: Retrospective analysis on 9562 cases of liquid-based cytology specimens were collected by our hospital from 2010-2011, cytology diagnosis using TBS (2001) classification reporting system; and 30 370 cases of Pap smears, cytology diagnosis using Palestine's five classification report; for the part of positive cytology cases, they were all under colposcopy with cervical biopsy and were sent for pathological diagnosis. Results: Among 9 562 cases of liquid-based, the positive were 403 cases, the detection rate was 4.21%, the pathological diagnosis of biopsy-positive rate is 90.3%; Among 30 370 cases of Pap smear, the positive were 187 cases, the detection rate was 0.62%, the pathological diagnosis of biopsy-positive rate is 88.2%. Conclusion: The positive detection rate of liquid-based cytology is much higher than that of Pap smear, but there is no obvious difference in pathological diagnosis of biopsy-positive rate between liquid-based cytology and Pap smear.%目的:探讨液基细胞学(TCT)和巴氏涂片检查在普通人群宫颈癌筛查中的价值.方法:回顾性分析2010-2011年本院收集的TCT标本9 562例,细胞学诊断以TBS (2001)分级系统报告;巴氏涂片标本30 370例,细胞学诊断以巴氏五级分类法报告;对细胞学阳性者,在阴道镜下行宫颈活检,送病理诊断.结果:9 562例TCT标本中阳性者403例,检出率为4.21%,和组织病理学诊断阳性符合率为90.3%;30 370例巴氏涂片标本中阳性者187例,检出率为0.62%,和组织病理学诊断阳性符合率为88.2%.结论:TCT阳性检出率明显高于巴氏涂片组,和组织病理学诊断阳性符合率两者无明显差异.

  7. Management of the abnormal Papanicolaou smear and colposcopy in pregnancy: an evidenced-based review.

    Science.gov (United States)

    Fleury, A C; Birsner, M L; Fader, A N

    2012-04-01

    Women diagnosed with abnormal Papanicolau smears or cervical abnormalities during pregnancy present a challenge to health care providers, as conventional management guidelines appropriate for the non-pregnant population may be contraindicated. The physiologic effects of pregnancy that may result in greater difficulty with the colposcopic examination include increased cervical mucus production that may obscure visualization, cervical hyperemia, gland prominence, and eversion of the columnar epithelium. The squamo-columnar junction may also be difficult to visualize in early pregnancy, but will often evert as the pregnancy continues. Because of these changes, cervical dysplasia may have a more prominent appearance in the gravid patient. Therefore, colposcopy should be performed by a skilled examiner with expertise in the cervical changes of pregnancy. The primary goal of colposcopy during pregnancy is to exclude the presence of invasive cancer, and thus, many cervical lesions may be followed with serial cytology and colposcopy during pregnancy or by deferring further colposcopic examination until the postpartum period. Cervical biopsy should be avoided unless a malignancy is suspected and endocervical sampling is contraindicated. Herein, we present a contemporary, evidence-based review of the colposcopic examination and guidelines for triaging and evaluating abnormal cervical cytology and lesions that are diagnosed during pregnancy.

  8. 高危型 HPV 结合液基细胞学技术在宫颈癌前病变诊断中的应用%Application of High-risk HPV Combined with Liquid-based Cytology Technology in the Diagnosis of Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    颜萍; 龚旭华

    2014-01-01

    Objective To investigate the application of high-risk HPV genotyping assay combined with liquid-based cy-tology for cervical lesions .Methods Liquid-based cytology samples of 204 cases of cervical cancer were detected by high-risk HPV genotyping assay ,and were compared with cervical biopsy results .Results 138 patients were TCT-positive combined with HPV infection,LSIL showed that HPV-positive was 40 cases (29.0%),HSIL showed that HPV-positive was 10 cases (7.2%), ASCUS showed that HPV-positive was 80 cases(58.0%),SCC showed that HPV-positive was 8 cases (5.8%).High-risk HPV was 42 cases,and positive rate was 30.4%.Samples of different types of high-risk HPV infection had significant difference ( P<0.05).When TCT and HPV were both ( -),there had no high grade lesion .When HPV-positive and TCT abnormal both oc-curred,detection rate of ≥CINⅠwas 34.8%.When TCT was abnormal HPV (-),the detection rate was 47.8%.Conclusion High-risk HPV genotyping assay combined with liquid-based cervical cytology is a valuable method for early screening of cervical cancer,the result is more reliable than single inspection .%目的:探讨高危型HPV基因分型检测结合液基细胞学检查在宫颈癌前病变诊断中的应用。方法纳入204例宫颈癌患者,采集液基细胞标本后,进行高危型HPV基因分型检测,并与宫颈活检结果进行比较分析。结果138例患者显示TCT阳性合并HPV感染,其中LSIL显示HPV阳性40例(29.0%),HSIL显示HPV阳性10例(7.2%),AS-CUS显示HPV阳性80例(58.0%),SCC显示HPV阳性8例(5.8%)。42例HPV高危型,阳性率为30.4%。不同类型标本HPV高危型感染率具有显著性差异( P<0.05)。 TCT和HPV均为(-)时,则未发生高度病变。 HPV阳性和TCT异常同时存在时,≥CINⅠ的检出率为34.8%。仅TCT异常而HPV(-)时,检出率为47.8%。结论高危型HPV基因分型检测结合液基细胞学检查是极具价

  9. Clinical value of liquid-based thinPrep cytology test and cervical biopsy in the diagnosis of cervical Squamous epithelial lesions%液基薄层细胞学检测联合宫颈活检对诊断宫颈鳞状上皮病变的临床价值

    Institute of Scientific and Technical Information of China (English)

    赵瑞皎; 石曼丽; 张家兴; 孔令非

    2012-01-01

    目的:探讨液基薄层细胞学检测(liquid-based thinPrep cytology test,TCT)联合宫颈活检对诊断宫颈鳞状上皮病变的临床价值.方法:应用TCT对30350例受检者进行宫颁细胞学检查,细胞学检查结果为鳞状上皮异常者,进行阴道镜活检及病理检查.结果:TCT检出鳞状上皮异常者1 824例(6.01%),其中无明确意义的不典型鳞状细胞(atypical squamous cells of undetermined significance,ASC-US)1423例,不除外高度鳞状上皮病变的不典型鳞状细胞(atypical squamous cells cannot exclude high grade intraepithelial lesion,ASC-H)214例,低度鳞状上皮内病变(low grade squamous intraepithelial lesion,LSIL) 92例,高度鳞状上皮内病变(high grade squamous intraepithelial lesion,HSIL) 80例,鳞状细胞癌(squamous cell carcinoma,SCC) 15例.与活检病理检测结果相比,1423例ASC-US中,宫颁上皮内瘤样病变-Ⅰ级(grade Ⅰ cervical intraepithelial neoplasia,CIN-Ⅰ)202例、CIN- Ⅱ和CIN- Ⅲ 22例、SCC 1例;214例ASC-H中,CIN-Ⅰ 12例、CIN-Ⅱ和CIN-Ⅲ 101例、SCC5例:LSIL、HSIL及SCC组中与组织病理检测结果的符合率分别为63.04%( 58/92)、81.25% (65/80)及100%( 15/15),SCC组和HSIL组的组织学符合率高于LSIL组(P<0.01).结论:TCT与阴道镜活检病理检测结果有较高的符合率,二者联合能提高宫颈癌前病变及癌变的检出率.%Objective : To investigate the clinical value of liquid-based thinPrep cytology test (TCT) and cervical biopsy in the diagnosis of cervical squamous epithelial lesions. Methods: TCT was performed in 30 350 subjects, and the colposcopy-directed biopsy and histopathologicai examination were performed when the TCT revealed abnormal cervical squamous epithelial lesions. Results: TCT results revealed that 1 824 (6.01%) cases had abnormal cervical squamous epithelial lesions, including 1 423 cases of atypical squamous cells of undetermined significance (ASC-US), 214 cases of atypical squamous cells cannot

  10. Clinical Application of Colposcopy Cervical Biopsy and Liquid-based Cytology Test in the Screening of Cervical Lesions%阴道镜宫颈活检与 TCT 联合检测在宫颈病变中的应用

    Institute of Scientific and Technical Information of China (English)

    冷海娜; 孙名芳

    2013-01-01

    Objective To explore the clinical application value of colposcopy cervical biopsy and liquid -based cytology test in the screening of cervical lesions .Methods Four hundred and eighty-three patients who udergone the TCT examination from January to December in gynecological clinic were chosen for pathological colposcopy cervical biopsy To select 483 gynecological clinic patients who had the TCT examination and were inspected to be abnormal in Care Hospital from January to December ,2012 ,have pathological colposcopy cervical biopsy for them and retrospective a-nalysis fwas carried out .or their entire clinical datum .Among which the TBS diagnostic system wasis used in the TCT in-spection report ,which contributed to the diagnosis ,as well as combined with the pathological examination report to make the diagnosis.Results Among the patients with the TCT result being ASC-US351,CINⅠ~CINⅢ223 patients(63. 53%) were detected with the pathological colposcopy cervical biopsy;CINⅠ~CINⅢ75 patients(91.46%) were de-tected with the pathological colposcopy cervical biopsy;CINⅠ~CIMⅢ41 patients were detected with the pathological colposcopy cervical biopsy and among the 6 SCC patients ,SCC 6 patients were detected with the colposcopy cervical bi-opsy .Conclusion The diagnose accordance rate of TCT examination and cervical biopsy diagnosis is high ;the diagnose accordance rate of cervical cancer with CIN of higher level is high .Precancerous lesions of uterine cervix can be found at an earlier age with the adoption of colposcopic cervical biopsy combined with TCT examination and stopped or slowed down ,which is the safe ,reliable and accurate examination method for the screening of cervical cancer .%目的:探讨阴道镜宫颈活检与宫颈薄层液基细胞学( TCT )在宫颈病变筛查中的临床应用价值。方法选取2012年1月~12月在妇科门诊行宫颈薄层液基细胞学( TCT)检查异常的483例患者,并同时行阴道镜宫颈活检

  11. Genomic amplification patterns of human telomerase RNA gene and C-MYC in liquid-based cytological specimens used for the detection of high-grade cervical intraepithelial neoplasia

    Directory of Open Access Journals (Sweden)

    Chen Shaomin

    2012-04-01

    Full Text Available Abstract Background The amplification of oncogenes initiated by high-risk human papillomavirus (HPV infection is an early event in cervical carcinogenesis and can be used for cervical lesion diagnosis. We measured the genomic amplification rates and the patterns of human telomerase RNA gene (TERC and C-MYC in the liquid-based cytological specimens to evaluate the diagnostic characteristics for the detection of high-grade cervical lesions. Methods Two hundred and forty-three residual cytological specimens were obtained from outpatients aged 25 to 64 years at Qilu Hospital, Shandong University. The specimens were evaluated by fluorescence in situ hybridization (FISH using chromosome probes to TERC (3q26 and C-MYC (8q24. All of the patients underwent colposcopic examination and histological evaluation. A Chi-square test was used for categorical data analysis. Results In the normal, cervical intraepithelial neoplasia grade 1 (CIN1, grade 2 (CIN2, grade 3 (CIN3 and squamous cervical cancer (SCC cases, the TERC positive rates were 9.2%, 17.2%, 76.2%, 100.0% and 100.0%, respectively; the C-MYC positive rates were 20.7%, 31.0%, 71.4%, 81.8% and 100.0%, respectively. The TERC and C-MYC positive rates were higher in the CIN2+ (CIN2, CIN3 and SCC cases than in the normal and CIN1 cases (p p p > 0.05. Conclusions The TERC test is highly sensitive and is therefore suitable for cervical cancer screening. The C-MYC test is not suitable for cancer screening because of its lower sensitivity. The amplification patterns of TERC become more diverse and complex as the severity of cervical diseases increases, whereas for C-MYC, the amplification patterns are similar between the normal/CIN1 and CIN2+ groups. Virtual slides The virtual slide(s for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1308004512669913.

  12. 颈淋巴结穿刺细胞学检查对颈淋巴结转移的诊断价值%Clinical value of aspiration cytology of cervical lymph node in the diagnosis of cervical lymph node metastasis

    Institute of Scientific and Technical Information of China (English)

    刘新梅

    2016-01-01

    Objective To investigate the clinical value of aspiration cytology of cervical lymph node in the diagnosis of cervical lymph node metastasis.Methods 80 cases of thyroid cancer with cervical lymph node metastasis admitted in our hospital from January 2003 to February 2015 were randomly selected.All patients were treated with fine needle aspiration cytology of cervical lymph node;the results were observed and recorded, and then compared with the routine postoperative pathological diagnosis results for analysis.Results After 80 patients received fine needle aspiration cytology of cervical lymph node, 74 cases were diagnosed as metastasis of cervical lymph node of thyroid cancer, with the positive rate of 92.50%;and the sensitivity of aspiration cytology of cervical lymph node was higher than that of slide cytological examination (85.19% vs.33.33%), with statistically significant difference (P<0.05).Conclusion Aspiration cytology of cervical lymph node can provide accurate preoperative diagnosis for thyroid cancer patients with cervical lymph node metastasis, which is helpful for the decision of the surgical plan and the successful development of the treatment.%目的 探讨采用颈淋巴结穿刺细胞学检查进行颈淋巴结转移诊断的临床价值.方法 随机选取本院2003年1月至2015年2月收治的甲状腺癌颈淋巴结转移患者80例,所有患者均接受颈淋巴结细针穿刺细胞学检查,对患者的细胞学检查结果进行观察记录,并同术后常规病理诊断结果进行对比分析.结果 80例患者经颈淋巴结穿刺细胞学检查,其中74例患者诊断为存在甲状腺癌颈淋巴结转移,阳性率为92.50%;颈淋巴结穿刺细胞学检查的灵敏度(85.19%)明显高于甲状腺切片细胞学检查结果(33.33%),差异有统计学意义(P<0.05).结论 颈淋巴结穿刺细胞学检查可对甲状腺癌合并颈淋巴结转移患者进行准确的术前诊断,有助于手术方案的制定和治疗的

  13. BACTERIAL VAGINOSIS IS NOT IMPORTANT IN THE ETIOLOGY OF CERVICAL NEOPLASIA - A SURVEY ON WOMEN WITH DYSKARYOTIC SMEARS

    NARCIS (Netherlands)

    VANLEEUWEN, AM; PIETERS, WJLM; HOLLEMA, H; QUINT, WGV; BURGER, MPM

    1995-01-01

    Background and Objectives: It has been suggested that bacterial vaginosis may play a role in the etiology of cervical neoplasia. The authors analyzed the prevalence, risk factors, and impact on histologic changes of bacterial vaginosis in women with cytological abnormalities of the uterine cervix. M

  14. Value of Cervical Fluid Based Cytology and Vaginal Examination in Early Screening for Cervical Lesions%宫颈病变早期筛查中宫颈液基细胞学和阴道镜检查的应用价值探析

    Institute of Scientific and Technical Information of China (English)

    张书霞

    2015-01-01

    目的 研究和探析宫颈病变早期筛查中宫颈液基细胞学和阴道镜检查的应用价值. 方法 收集2013年1月-2014年12月到该院妇科门诊行宫颈检查的患者共1 037例,所有患者均接受宫颈液基细胞学检测,对检查结果呈阳性者进行阴道镜检查. 结果 37例(3.6%)患者宫颈液基细胞学检测呈阳性,经过阴道镜检查后,23例显示阳性,其中炎症12例(32.4%),宫颈上皮内瘤样病变Ⅰ期14例(37.8%), 宫颈上皮内瘤样病变Ⅱ~Ⅲ6例(16.2%). 阴道镜下的活检组织病理学检查结果与宫颈液基细胞学的检查结果的差异有统计学意义(P<0.05).结论 在宫颈病变早期筛查中,宫颈液基细胞学和阴道镜活组织病理检查联合使用,有助于尽早发现宫颈病变,值得推广应用.%Objective To study the application value of cervical fluid based cytology and vaginal examination in early screening for cervical lesions. Methods 1037 gynecological outpatients who underwent inspection of the cervix in the hospital between January 2013 and December 2014 were included. All of them received cervical fluid based cytology, and for those with positive results vaginoscopy was carried out. Results Out of 37 patients (3.6%) who showed positive result from cervical fluid based cytology, 23 showed positive result after vaginoscopy, including 12 (32.4%) cases of f inflammation and 14(37.8%) cases of stage I cervical in-traepithelial neoplasia, and 6 (16.2%) cases of stage II-III cervical intraepithelial neoplasia. Therefore, there was statistically sig-nificant difference in the outcomes between vaginoscopy and cervical fluid based cytology, P<0.05. Conclusion In the early screening of cervical lesions, the cervical fluid based cytology combined with pathological examination of biopsy under vaginoscope is beneficial to the early detection of cervical lesions, therefore it is worthy of popularization and application.

  15. The value of liquid-based cytology combined with colposcopy in cervical cancer screening%液基细胞学联合阴道镜筛查在宫颈癌防治中的应用价值

    Institute of Scientific and Technical Information of China (English)

    刘晓艳

    2013-01-01

    Objective To investigate the value of liquid-based cytology combined with colposcopy in cervical cancer screening. Methods 400 cases were randomly divided into two groups, each 200 cases, the observation group used liquid-based cytology combined with colposcopy, the control group simply used liquid-based cytology, then pathological findings diagnostic results and analyzed the false positive rate and false negative rate were compared in the two groups. Results The observation group with cervical cancer diagnosed was 95.4%, and then were significantly higher than 63.1% in control group (P < 0.05), the observation group with false negative rates of false-positive rate was significantly lower than control group (P < 0.05). Conclusion The liquid-based cytology combination with colposcopy can improve cervical cancer screening rates and reduce the misdiagnosis and missed diagnosis rate, so it is worthy of promotion.%目的:探讨液基细胞学联合阴道镜筛查在宫颈癌防治中的应用价值。方法选择400例患者,随机分为两组,各200例,观察组使用液基细胞学联合阴道镜检术,对照组则单纯使用液基细胞学检查,比较两组患者诊断结果与病理检查结果,并分析假阳性率和假阴性率。结果观察组宫颈癌确诊率为95.4%,显著高于对照组的63.1%(P<0.05),观察组假阳性率和假阴性率均显著低于对照组(P<0.05)。结论液基细胞学检查联合阴道镜检能有效提高宫颈癌筛查率,减少误诊和漏诊率,值得临床推广。

  16. 观察HPV联合液基细胞学检查在宫颈癌筛查中的应用效果%To observe the effect of HPV combined with liquid based cytology testin cervical cancer screening

    Institute of Scientific and Technical Information of China (English)

    唐利华

    2015-01-01

    Objective Application of HPV in cervical cancer screening joint liquid based cytology effects were observed. Methods From the hospital outpatient service of department of gynaecology in from January 2012 to December 2012 received line in patients with cervical cancer screening randomly 320 cases were studied, and randomly divided into control group 160 cases, liquid based cytology examination and observation group 160 cases, HPV joint liquid based cytology, compare the positive detection rate of two groups of patients.Results The positive detection rate of 59.375% in the control group and observation group was 74.375%, by comparison, the observation group is significantly higher than the control group, the differences between groups (P<0.05). Conclusion Combined use of HPV in cervical cancer screening and liquid based cytology, can improve the diagnostic accuracy, has high clinical value.%目的:对宫颈癌筛查中应用HPV联合液基细胞学检查的效果进行观察。方法选取2012年1月~2013年12月在我院妇科门诊行宫颈癌筛查患者320例为研究对象,将其随机分成对照组和观察组,各160例。对照组行液基细胞学检查,观察组行HPV联合液基细胞学检查,比较两组患者的阳性检出率。结果对照组的阳性检出率为59.375%,观察组为74.375%,差异有统计学意义(P<0.05)。结论在宫颈癌筛查中联合应用HPV和液基细胞学检查,可以提高诊断准确率,具有较高的临床应用价值。

  17. Application of thinprep cytology test and cervical smear combined with colposcope in screening of cervical lesions%液基细胞学、宫颈刮片联合阴道镜在宫颈病变筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    江明珠

    2015-01-01

    目的 探讨液基细胞学(TCT)、宫颈刮片联合阴道镜在宫颈病变筛查中的应用价值. 方法 选取2014年1月~2015年4月来我所妇科门诊行TCT筛查的1726例妇女(门诊组)和在乡镇行宫颈刮片细胞学筛查的26 021例妇女(乡镇组)作为研究对象,两组均行TBS分级诊断,将细胞学阳性者进一步行阴道镜及镜下活组织病理学检查,分析两组不同取材方法的细胞学筛查结果,并观察细胞学阳性者的阴道镜和病理组织学检查结果. 结果 两组细胞学检出阳性率差异有统计学意义(X2=86.56,P0.05). 以病理组织学诊断为金标准,宫颈癌前病变和宫颈癌(CIN+SCC)两者合计,阴道镜诊断符合率为90.14%(128/142), 细胞学诊断符合率为70.73%(87/123). 结论 TCT筛查法优于传统刮片细胞学筛查法,联合应用阴道镜及镜下活检组织病理学检查,可明显提高宫颈癌前病变及宫颈癌的检出.%Objective To discuss the application value of thinprep cytology test(TCT) and cervical smear combined with colposcope in screening of cervical lesions. Methods 1726 cases of female patients (outpatient group) visited outpatient clinic in our hospital from January 2014 to April 2015 and 26 021 cases of rural women (rural group) receiving cervical cancer screening were selected as research object.Two groups were diagnosed by TBS classification,and those with pos-itive cytologic results were further examined by colposcope and histopathological examination.The cytologic screening results in two groups were analyzed by different methods, the colposcope and histopathologic results of patients with positive cytologic results were observed. Results There was significant difference in positive rate of cytologic results be-tween two groups (X2=86.56,P0.05).The gold standard was diagnosed by histopathology.The total of cervical precancerous lesions and cervical cancer (CIN+SCC),colposcope diagnosis coincidence rate was 90.14%(128/142),and cytology

  18. Human papillomavirus research on the prevention, diagnosis, and prognosis of cervical cancer in Taiwan.

    Science.gov (United States)

    Chao, Angel; Huang, Huei-Jean; Lai, Chyong-Huey

    2012-01-01

    Cervical cancer is third in incidence and fourth in mortality among cancers of women worldwide. Epidemiological studies have shown that human papillomavirus (HPV) is necessary, if not sufficient, to cause nearly 100% of cervical cancers. HPV testing is useful in primary screening for cervical neoplasms. The value of HPV detection or genotyping is potentially useful in triage of borderline or low-grade abnormal cervical cytology, follow-up after treatment of cervical intraepithelial neoplasia, assessment of prognosis and treatment planning for invasive cervical cancer. Studies from Chang Gung Memorial Hospital have defined the genotype distribution of cervical cancer in Taiwan and confirmed the independent prognostic value of the HPV genotype in cervical cancer. The cost-effectiveness of using HPV testing in prevention and management of cervical neoplasms depends on the medical and public health infrastructure of the individual country. The population-based HPV prevalence and genotype distribution as well as longitudinal follow-up studies have established strong support for incorporating HPV testing with cervical cytology and for future comparisons of HPV epidemiology before and after implementation of HPV prophylactic vaccines in Taiwan. Future directions in HPV research are discussed. PMID:22913856

  19. Computer-assisted myelography in degenerative abnormalities of the cervical vertebral column.

    Science.gov (United States)

    van der Tas, C

    1979-01-01

    In 19 patients with symptoms suggestive of a cervicobrachial syndrome due to degernative lesions of the cervical spine, a CT scan was made after intrathecal administration of metrizamide. The images thus obtained supply adequate information on the extent of bone apposition, if any, on the degree of displacement and compression of the spinal cord, and on the condition of the intervertebral foramina. A CT scan is indicated in the case of discrepancy between myelographic findings and clinical diagnosis. In the case of recurrent postoperative symptoms, too, CT scans have proved to supply valuable information. PMID:535511

  20. Telomerase activity as a biomarker for (pre)neoplastic cervical disease in scrapings and frozen sections from patients with abnormal cervical smear

    NARCIS (Netherlands)

    Wisman, GBA; Hollema, H; de Jong, S; ter Schegget, J; Tjong-A-Hung, SP; Ruiters, MHJ; Krans, M; de Vries, EGE; van der Zee, AGJ

    1998-01-01

    Purpose: To evaluate the diagnostic value of semiquantitative telomerase activity assessment in cervical scrapings together with human papillomavirus (HPV) typing for detection of (pre)neoplastic cervical lesions and to compare telomerase activity in cervical scrapings and frozen specimens from the

  1. Detection of gene amplification in MYCN, C-MYC, MYCL1, ERBB2, EGFR, AKT2, and human papilloma virus in samples from cervical smear normal cytology, intraepithelial cervical neoplasia (CIN I, II, III, and cervical cancer

    Directory of Open Access Journals (Sweden)

    Dabeiba Adriana García

    2011-06-01

    Full Text Available Introducción: El cáncer cervical es el segundo cáncer más importante en mujeres a nivel mundial y es la segunda causa de muerte por cáncer en mujeres. Se ha demostrado que el proceso de carcinogénesis cervical presenta componentes tanto genéticos como epigenéticos y medio ambientales. En la actualidad, hay gran interés en la búsqueda de marcadores moleculares asociados con la progresión de esta enfermedad, uno de los posibles mecanismos y que además está poco estudiado en cáncer cervical es la amplificación génica de algunos oncogenes como la familia MYC, EGFR y AKT entre otros. Objetivos: Detectar la amplificación génica de MYCN, C-MYC, MYCL1, ERBB2, EGFR y AKT2 además de la presencia del virus de papiloma humano en cepillados cervicales en mujeres con citología normal o con neoplasia intraepitelial cervical (NIC I, II y III o con cáncer cervical. Métodos: Se genotipificó mediante reverse line blot (RLB el virus de papiloma humano (VPH y se determinó el estado de amplificación génica de los genes mencionados mediante PCR en tiempo real utilizando sondas taqman. Resultados: El VPH se encontró presente en 4% de las pacientes con citología normal, en 48% en NIC I, 63.6% en NIC II, 64% en NIC III y 70.8% en cáncer cervical. Los genes MYCN, MYCL1 y ERBB2 mostraron mayor amplificación en lesiones de alto grado y cáncer con diferencias estadísticamente significativas  a las lesiones de bajo grado y citología normal, en 39.1%, 34.7% y 30.4% respectivamente. Además, se encontraron amplificados los genes C-MYC, EGFR y AKT2, en muestras de pacientes con cáncer cervical, en 12%, 18% y 13% respectivamente. Sin embargo, no se observaron diferencias estadísticamente significativas con respecto a las lesiones de alto y bajo grado y citología normal. Conclusión: En las lesiones de alto grado como en cáncer cervical, se encuentra mayor prevalencia del virus al igual que se detectan mayor cantidad de alteraciones gen

  2. Value of computer-assisted slide-screening system in ThinPrep cervical cytology%电脑辅助阅片系统在宫颈液基细胞涂片诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    杨静; 陶琨; 杨华; 郭振华; 胡月梅; 谈振宇

    2013-01-01

    Objective To investigate the value of computer-assisted slide-screening system (ThinPrep imaging system,TIS) in the diagnosis of cervical Thinprep smears.Methods A total of 19 600 ThinPrep smears were collected,including 9800 slides by TIS-assisted screening from September 2011 to March 2012 and 9800 slides by manual screening from September 2010 to April 2011 as control.The detection rates of abnormal cells and common microbial infection by the different screening methods were compared.With histopathological diagnosis of colposcopic biopsy as the gold standard,the screening efficiency and correlation of cytologic diagnosis among different screening methods were analyzed.Results Compared with manual screening,the detection rate of abnormal cells in 9800 cases by TIS-assisted screen was increased from 5.4% (525/9800) to 6.8% (665/9800),mainly in the categories of ASCUS and LSIL (P < 0.05).TIS had a higher accordance rate between cytologic diagnosis and histopathological diagnosis in the NILM and ASCUS than that by manual screening.False-negative rate of finding abnormal cells by TIS decreased from 8.5% (17/200) to 0.7% (2/289,P < 0.01) with an increased sensitivity compared to manual screening,althoughthe specificity was similar.Both TIS and manual screening had advantages and disadvantages respectively in the detection of microbial organisms.TIS improved screening efficiency by 50%.Conclusion TIS improves not only the screening efficiency but also the detection of abnormal cells with a reduced false negativity,and it therefore has a broad application prospect.%目的 探讨电脑辅助阅片系统(TIS)在宫颈薄层液基细胞涂片诊断中的价值.方法 共收集19 600例液基细胞涂片,其中2011年9月至2012年3月期间9800例经TIS阅片,2010年9月至2011年4月期间9800例经人工阅片作为对照,比较两种阅片方法异常细胞检出率和常见微生物检出率;以阴道镜活检的组织病理学诊断结果作为金标

  3. 不同细胞学检查方法在宫颈癌筛查中的应用%Applications of different methods for cytological examination in cervical cancer screening

    Institute of Scientific and Technical Information of China (English)

    张敏; 张丽华; 胡争光; 徐爱娣

    2012-01-01

    Objective; To evaluate the effects of different methods for cytological examination in cervical cancer screening, explore the appropriate techniques for screening of cervical cancer, in order to conduct early diagnosis and early treatment, improve the detection rate of precancgrous lesions of uterine cervix. Methods: Liquid - based cytological test and traditional Papanicolaou smear were used for cervical cancer screening among 1 091 cases and 4 784 cases respectively from October 2005 to June 2007, then the cases with positive results of cytological examination underwent biopsy under colposcope and pathological examination. Results: The sensitivity, specificity, negative predictive value, positive predictive value, and coincidence rate of traditional Papanicolaou smear were 44. 44% , 83. 33% , 30. 0% , 83. 33% , and 77. 95 % , respectively; the sensitivity, specificity, negative predictive value, positive predictive value, and coincidence rate of traditional Papanicolaou smear were 92. 11% , 88. 37% , 77. 78, 88. 37% , and 89. 52% , respectively. The detection rate of precancerous lesions of uterine cervix by liquid - based cytological test was 91. 67% , which was significantly higher than that by traditional Papanicolaou smear (40. 00% ) ( P < 0. 05) . Conclusion: The sensitivity of liquid - based cytological test is significantly higher than that of traditional Papanicolaou smear, liquid - based cytological test can improve the detection rate obviously, especially for the cases with low - grade squa-mous intraepithelial lesion (LSIL) and high -grade squamous intraepithelial lesion (HSIL) .%目的:评价不同细胞学检查方法在宫颈癌筛查中的作用,探讨宫颈癌筛查的适宜技术,以便做到早诊、早治,有效提高宫颈癌前病变的检出率.方法:采用液基薄层细胞学检测技术(LCT)及传统宫颈细胞涂片(巴氏涂片)法,分别对2005年10月~ 2007年6月1 091名及4 784名受检者进行宫颈癌筛查,根据细胞学

  4. Validade da citologia e da biópsia orientada pela colposcopia no diagnóstico do carcinoma cervical pré-clínico Validity of cytology and colposcopy - guided biopsy for the diagnosis of preclinical cervical carcinoma

    Directory of Open Access Journals (Sweden)

    Aldo Franklin Ferreira Reis

    1999-05-01

    ,5 para displasia acentuada e carcinoma in situ, zero para displasia leve e moderada, zero para negativo e inflamatório e 0,4 para o conjunto de resultados negativos para invasão.Purpose: to evaluate the efficacy of cytology and colposcopy-directed biopsy to distinguish preclinical invasive cervical carcinoma from intraepithelial lesions. Patients and Methods: 441 patients submitted to conization, hysterectomy and Wertheim-Meigs operation from 1978 to 1995 in the University Hospital "Clementino Fraga Filho", Federal University of Rio de Janeiro, Cervical Pathology Outpatient Clinic. We estimated sensitivity, specificity, predictive values, likelihood ratio and confidence intervals of each study, which were divided into four classes: 1 normal and inflammatory; 2 mild and moderate dysplasias; 3 severe dysplasia and carcinoma in situ; 4 microcarcinoma and invasive carcinoma. Biopsies were analyzed as a whole and separated in accordance with the type of the colposcopic result (satisfactory and unsatisfactory. Results: cytology has shown sensitivity of 50%, specificity of 89%, positive predictive value of 63% and negative predictive value of 82%. The likelihood ratios were 4.4 for stromal invasion diagnosis, 0.7 for severe dysplasia and carcinoma in situ, 0.1 for mild and moderate dysplasia, 2.2 for normal and inflammatory report and 0.6 for the negative results for invasion as a whole. Satisfactory colposcopic guided biopsy white a visible lesion showed sensitivity of 59%, specificity of 100% positive predictive value of 100% and negative predictive value of 83%. Likelyohood ratios were: tending to infinity for invasion, 0.5 for severe dysplasia and carcinoma in situ, zero for mild and moderate dysplasia, zero for negative and inflammatory and 0.4 for all negative results for invasion.

  5. The PapilloCheck Assay for Detection of High-Grade Cervical Intraepithelial Neoplasia.

    Science.gov (United States)

    Crosbie, Emma J; Bailey, Andrew; Sargent, Alex; Gilham, Clare; Peto, Julian; Kitchener, Henry C

    2015-11-01

    Human papillomavirus (HPV) testing is used in primary cervical screening, as an adjunct to cervical cytology for the management of low grade abnormal cytology, and in a test of cure. PapilloCheck (Greiner Bio-One) is a PCR-based DNA microarray system that can individually identify 24 HPV types, including the 13 high-risk (HR) types identified by Hybrid Capture 2 (HC2). Here, we compare PapilloCheck with HC2 for the detection of high-grade cervical intraepithelial neoplasia (CIN2+) in a total of 8,610 cervical cytology samples from the ARTISTIC population-based cervical screening study. We performed a retrospective analysis of 3,518 cytology samples from round 1 ARTISTIC enriched for underlying CIN2+ (n = 723) and a prospective analysis of 5,092 samples from round 3 ARTISTIC. Discrepant results were tested using the Roche reverse line blot (RLB) or Linear Array (LA) assay. The relative sensitivity and specificity of HR PapilloCheck compared with that of HC2 for the detection of CIN2+ in women aged over 30 years were 0.94 (95% confidence interval [CI], 0.91, 0.97) and 1.05 (95% CI, 1.04, 1.05), respectively. HC2 missed 44/672 (7%) CIN2+ lesions, while HR PapilloCheck missed 74/672 (11%) CIN2+ lesions. Thirty-six percent of HC2-positive normal cytology samples were HR HPV negative by both PapilloCheck and RLB/LA, indicating that the use of HR PapilloCheck rather than HC2 in population-based primary screening would reduce the number of additional tests required (e.g., reflex cytology) in women where underlying CIN2+ is extremely unlikely. HR PapilloCheck could be a suitable HPV detection assay for use in the cervical screening setting. PMID:26338859

  6. Cervical screening in HIV-positive women in the East of England: recent CD4 as the predictive risk factor.

    Science.gov (United States)

    Davies, Olubanke; Rajamanoharan, Sasikala; Balachandran, Thambiah

    2015-11-01

    This study examines the relationship between CD4 count and cervical cytological abnormality in HIV-positive women attending two district general hospital genitourinary medicine clinics in the East of England. It aims to determine whether the rate of cervical cytological abnormalities differs in HIV-positive women with CD4 count >350 cells/µl and those with CD4 count ≤350 cells/µl; and to compare the rates of abnormalities with that of the general population. We retrospectively reviewed data from a cross-sectional audit undertaken between December 2010 and December 2011 and analysed them using multivariable statistics. There was a significant association between recent CD4 count ≤350 cells/µl and cervical cytological abnormality (p 350 cells/µl had abnormal cervical smear results, compared with 6.6% of the general population in the screening period 2010-11 and 7.2% of the general population in the screening period 2009-10. In our study population of women with recent CD4 counts >350 cells/µl, the proportions of mild, moderate and severe dysplasia were also similar to national figures. This raises important questions about the cost effectiveness of blanket annual screening for HIV-positive women.

  7. A evaluation on cervical intraepithelial lesion and cervical cancer screening by DNA quantitative analysis and liquid-based monolayer cytology%液基薄层细胞学联合DNA定量方法对宫颈病变诊断试验的评价

    Institute of Scientific and Technical Information of China (English)

    侯安丽; 张玉娟; 李秀芬; 邵雪斋; 王杏茶

    2012-01-01

    目的 评价采用液基薄层细胞学联合DNA定量分析方法进行宫颈癌普查的工作效率.方法 对参与宫颈癌普查的2 599名妇女用宫颈刷取材,进行液基薄层制片,分别进行巴氏染色和Feulgen染色,由细胞学医师对巴氏染色片做细胞学诊断,应用全自动DNA定量分析对Feulgen染色片进行自动扫描诊断.其中156例进一步做了宫颈组织病理诊断.结果 DNA定量分析方法的敏感度、特异度、准确度、阳性预测值、阴性预测值、阳性似然比均高于液基薄层细胞学方法,而误诊率、漏诊率及阴性似然比均低于液基薄层细胞学方法.液基薄层细胞学和DNA定量分析方法的平行诊断试验的联合敏感度和联合特异度分别是99.56%、44.52%,两种方法的系列诊断试验的联合敏感度和联合特异度分别是83.78%、89.61%.平行诊断试验的敏感度最高,系列诊断试验的特异度最高.结论 DNA定量分析方法与液基薄层细胞学联合筛查,可提高宫颈癌前病变及宫颈癌筛查的敏感度和特异度.%Objective To evaluate the working efficiency of cervical cancer screening by combining DNA quantitative analysis with liquid-based monoiaycr cytology. Methods A total of 2599 women were involved in this study and cervical samples were taken by a cervical brush. Monolaycr of cells were deposited onto microscopic slides by liquid-based sampling preparation. Two slides were prepared from each case: one slide was stained by Papanicolaou stain for conventional cytology examination, while the other slide was stained by Fculgcn method for determination of amount of DNA using an automated DNA imaging cytomctry. 156 women of the total were followed by pathological examination of cervical tissue. Results The sensitivity, specificity, accuracy, positive predictive value, negative predictive value and positive likelihood ratio of DNA quantitative analysis arc higher than liquid-based monolaycr cytology,however the

  8. 液基细胞学检查与HPV定量分析在宫颈癌普查中的应用%Application of Thinprip Cytologic Test Combined with Cervical HPV Quantitative Analysis in the General Survey of Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    黎骋

    2015-01-01

    目的::探讨宫颈液基细胞学检查与宫颈HPV定量分析联合应用于宫颈癌普查中的临床病理意义。方法:回顾性分析2010年3月至2014年3月的1000例妇产科患者的临床资料,包括患者的门诊资料和住院病历数据,记录、比较液基细胞检查、HPV定量分析及联合诊断的准确率,总结相关数据的临床意义。结果:HPV感染率与各级宫颈病变和年龄段之间存在统计学差异( P<0.05)。液基细胞学检查准确率(79.60%)低于宫颈HPV定量分析准确率(81.00%)和联合检查准确率(98.50%),宫颈液基细胞学检查联合宫颈HPV定量分析的临床准确率显著高于宫颈液基细胞学检查、宫颈HPV定量分析的单独准确率(P<0.05)。结论:较之单独的宫颈液基细胞学检查和宫颈HPV定量分析,二者联合具有极高的诊断准确率,为子宫颈癌普查中的有效方法,可在临床筛查中进一步推广应用。%Objective:To discuss the clinicopathological significance of applying thinprip cytologic test (TCT) combined with cervical HPV quantitative analysis in the general survey of cervical cancer. Methods:Retrospectively analyzed the clinical datas of 1 000 patients in obstetrics and gynecology department from Mar 2010 to Mar 2014;the outpatient datas, hospital cases, TCT, HPV quantitative analysis and diagnosis were recorded and compared to; and summarized the clinical significance of the related datas. Results:There were differences in HPV infection rate,all level cervical lesions and age groups (P<0. 05). The accuracy of TCT (79. 60%) was lower than cervical HPV quantitative analysis (81. 09%) and combined diagnosis (98. 51%). Clinical accuracy of liquid-based cervical cytology combined with quantitative analysis of cervical HPV was significantly greater than that of liquid-based cervical cytology or cervical HPV quantitative analysis alone (P<0. 05). Conclusion:TCT combined with cervical HPV quantitative

  9. Clinical Value of Liquid-based Cytology in the Diagnosis of Cervical Lesions%液基细胞学检查在诊断宫颈病变中的临床价值

    Institute of Scientific and Technical Information of China (English)

    姜丽红; 杨滨

    2011-01-01

    [目的]探讨宫颈液基细胞学检查(TCT)对宫颈病变的诊断价值及意义.[方法]回顾性分析中国医科大学附属盛京医院门诊57 784例TCT筛查结果.细胞学诊断采用TBS(2001)分级报告系统,阳性诊断包括意义不明确的不典型鳞状细胞(ASC-US)及以上病变,其中阳性者554例行阴道镜下宫颈活组织检查,其中317例同时又进行了宫颈高危型人乳头瘤病毒DNA(HPV-DNA)检测,对三者进行了对比分析.另外对经宫颈活检证实为宫颈癌并同时有TCT检测结果的103例患者结果也进行了对比分析.[结果]①TCT检查阳性结果与宫颈活检诊断符合率:鳞状细胞癌(SCC)3例及腺癌4例均符合组织病理学诊断;高级别鳞状上皮内病变(HSIL)符合率为86.67%(91/105),低级别鳞状上皮内瘤变(LSIL)符合率为33.93% (38/112);②经宫颈活检证实为原位癌和SCC患者TCT与宫颈活检诊断的符合率为28.57%(26/91),假阴性率38.46%(35/91),经宫颈活检证实为腺癌患者TCT与宫颈活检诊断的符合率为50.00%(6/12),假阴性率41.67%(5/12);③TCT提示HPV感染与宫颈HPV-DNA检测的符合率为79.00%(173/219);④宫颈高危型HPV-DNA 阳性检出率与组织病理学分级正相关.[结论]TCT用于宫颈病变筛查,能够及时发现大量的宫颈病变,TCT结果为HSIL及宫颈癌的患者与宫颈活检诊断的符合率较高.TCT提示HPV感染有较高的准确性,但TCT对宫颈癌筛查有较高的假阴性率.对于TCT检查阴性、而临床高度可疑恶性病变、年龄较大的患者应予以重视,进一步做阴道镜检查及高危型HPV-DNA检测和宫颈活组织检查,尤其是宫颈管诊刮,以降低宫颈癌的漏诊率.高危型HPV-DNA检测可以作为TCT诊断的客观控制指标,还可作为ASC-US及LSIL患者分流无病与患病人群的检查.%[Objective] To explore the value of liquid-based cervical cytology(TCT) for the diagnosis of cervical lesions and its significance. [Methods] The TCT

  10. 探讨宫颈液基细胞学与HPV检测在宫颈癌筛查中的联合作用%The Discussion about the Combined Effect of Cervical Liquid Based Cytology and HPV Test in Screening for Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    李琴艳; 付雯; 刘蔚; 陈加来

    2014-01-01

    Objective:To discuss the application value of Thin prep cytologic test and high-risk human papilloma virus HPV-DNA test in the diagnosis of cervical lesions. Method:1194 patients in our hospital department of gynaecology clinic were selected as the research object,who should do cervical liquid based cytology and HPV test routinely. Cytology positive or HPV-DNA positive did pathology biopsy test under colposcope and regarded the pathology diagnosis as the gold standard. Result:The sensitivity,specificity,accuracy,and Youden index of each diagnostic test was listed respectively as follows:89.66%,90.49%,90.45%,0.801 for liquid-based cytology,79.31%,91.90%,91.29%,0.712 for HPV DNA test,94.83%,89.70%,89.95%,0.845 for combine liquid-based cytology and HPV DNA test in parallel,87.93%, 95.16%,94.81%,0.831 for combine liquid-based cytology and HPV DNA test in series. The diagnosis value of combine liquid-based cytology and HPV DNA test in series and in parallel was higher than the others through the analysis of the area under ROC curve. Conclusion:LPT combined with HPV-DNA testing for cervical cancer screening can improve not only the sensitivity and specificity of cervical cancer and precancerous lesions,but also detection rate.%目的:探讨液基细胞学检查(LPT)与高危型人乳头状瘤病毒HPV-DNA检测在子宫颈病变诊断中的应用价值。方法:选取1194例在本院妇科门诊就诊的妇女作为研究对象,常规行宫颈液基细胞学及HPV-DNA检查,以活检的病理诊断为金标准。结果:以组织病理学结果作为标准,液基细胞学检查方法的敏感性、特异性、准确性及约登指数分别为89.66%、90.49%、90.45%、0.801,HPV-DNA检测方法的敏感性、特异性、准确性及约登指数分别为79.31%、91.90%、91.29%、0.712,并联液基细胞学检查和HPV DNA检测方法的敏感性、特异性、准确性及约登指数分别为94.83%、89.70%、89.95%、0.845,串

  11. Human papillomavirus prevalence in paired urine and cervical samples in women invited for cervical cancer screening.

    Science.gov (United States)

    Burroni, Elena; Bonanni, Paolo; Sani, Cristina; Lastrucci, Vieri; Carozzi, Francesca; Iossa, Anna; Andersson, Karin Louise; Brandigi, Livia; Di Pierro, Carmelina; Confortini, Massimo; Levi, Miriam; Boccalini, Sara; Indiani, Laura; Sala, Antonino; Tanini, Tommaso; Bechini, Angela; Azzari, Chiara

    2015-03-01

    With the introduction of Human papillomavirus (HPV) vaccination in young girls in 2007, it is important to monitor HPV infections and epidemiological changes in this target population. The present study has evaluated the detection of human papillomavirus DNA in paired cervical and urine samples to understand if HPV testing in urine could be used as non-invasive method to monitor HPV status in young women. The study enrolled 216 twenty five-year-old women, resident in Florence and invited for the first time to the cervical cancer Screening Program within a project evaluating the impact of HPV vaccination. HPV genotyping was performed on 216 paired urine and cervical samples. The overall concordance between cervix and urine samples, investigated by HPV genotyping (INNO-LiPA HPV Genotyping Extra), was: 85.6% (184/215), 84.6% (182/215), 80% (172/215) when the same HPV, at least the same HR HPV and all HR HPV, respectively, were detected. HPV type specific concordance in paired urine and cervical samples was observed in 85.8% (175/204) of women with normal cytology and in seven out of nine women with abnormal cytology. Urine seems to be a suitable and reliable biological material for HPV DNA detection as evidenced by the high concordance with HPV detected in cervical samples. These results suggest that urine could be a good noninvasive tool to monitor HPV infection in vaccinated women.

  12. Application of liquid -based cytology with colposcopy in diagnosis of cervical lesions%液基细胞学结合阴道镜检查在诊断宫颈病变中的应用

    Institute of Scientific and Technical Information of China (English)

    周晓花

    2011-01-01

    Objective To explore and evaluate the liquid -based cytology(TCT) and colposcopy for cervical lesions.Methods The diagnostic criteria for pathological results in our hospital colposcopy, biopsy and the cervical lesions TCT test results were analyzed.Results TCT compared with the biopsy tissue, benign coincidence rate was 98.4% , LSIL, HSIL, cancer diagnosis rate was 85.4% ,97% and 100%; Colposcopy compared with histologica findings, CIN or more colposcopy cervical lesions was 97.8% compliance.Conclusions TCT consistent with colposcopy was no significant difference( P > 0.05 ), application of TCT assisted colposcopy can improve cervical lesions and cancer detection rate.%目的 探讨和评价液基细胞学(TCT)结合阴道镜对宫颈病变的诊断价值.方法 以病理学结果为诊断标准,对在永城市人民医院进行阴道镜、活检及TCT的宫颈病变患者的检查结果进行分析.结果 TCT与活检病理组织学相比,良性符合率为98.4%,LSIL、HSIL、癌的诊断符合率分别为85.4%、97%和100%;阴道镜检查结果与组织学相比,CIN以上的宫颈病变阴道镜符合率为97.8%.结论 TCT与阴道镜检查符合率无统计学意义(P>0.05),应用TCT辅助阴道镜检查可提高宫颈癌前病变和癌变的检出率.

  13. The effect of human papillomavirus DNA testing and cervical cytology for screening of cervical precancerous lesions%人乳头瘤病毒DNA检测和宫颈细胞学检查对宫颈癌前病变筛查的效果

    Institute of Scientific and Technical Information of China (English)

    梁瑜; 彭华丽

    2015-01-01

    Objective To investigate the effect of human papillomavirus (HPV) DNA testing and cervical cytology screening of cervical precancerous lesions. Methods 1100 patients admitted to our hospital who were carried out screening cervical precancerous lesions from January 2013 to June 2014 were selected,all were carried out HPV DNA testing,cervical cytology,colposcopy and cervical biopsy,pathological diagnosis was as the standard,the test results were analyzed. Results Positive rate of HPV DNA testing was 23.00% in 1100 patients,in which,positive rate of cervical cancer was 83.33%,positive rate of cervical intraepithelial neoplasia (CIN) Ⅰ was 44.44%,CIN II testing was 86.67%, CINⅢ positive rate was 94.12%.Cervical cytology results showed that there were 851 cases (77.36%) with no CIN,139 cases (12.64%) were atypical squamous cells,78 cases (7.09%) were low-grade squamous intraepithelial lesion,29 cases (2.64%) of high-grade squamous intraepithelial cells lesions,3 cases (0.27%) of squamous cell carcinoma.The compli-ance rate of colposcopy examination and pathological diagnosis was 62.79% (54/86). Conclusion The suitable cervical lesions screening method for patients can help to improve the detection rate,reduce the incidence rate of cervical cancer.%目的:探讨人乳头瘤病毒(HPV)DNA检测和宫颈细胞学检查对宫颈癌前病变筛查的效果。方法选取2013年1月~2014年6月来本院就诊并进行宫颈癌前病变筛查的1100例患者,均进行HPV DNA检测、宫颈细胞学检查、阴道镜检查与宫颈活检,以病理诊断为标准,对检测结果进行分析。结果1100例患者中,HPV DNA阳性率为23.00豫,其中宫颈癌阳性率为83.33豫,宫颈上皮内瘤变(CIN)Ⅰ阳性率为44.44豫,CINⅡ阳性率为86.67豫,CINⅢ阳性率为94.12豫。宫颈细胞学检查无CIN 851例(77.36豫),非典型鳞状上皮细胞139例(12.64豫),低度鳞状上皮细胞内病变78例(7.09豫),高度鳞状上皮细胞内病变29

  14. Effect of human papillomavirus vaccination on cervical cancer screening in Alberta

    Science.gov (United States)

    Kim, Jong; Bell, Christopher; Sun, Maggie; Kliewer, Gordon; Xu, Linan; McInerney, Maria; Svenson, Lawrence W.; Yang, Huiming

    2016-01-01

    Background: A school-based program with quadrivalent human papillomavirus (HPV) vaccination was implemented in Alberta in 2008. We assessed the impact of this program on Pap test cytology results using databases of province-wide vaccination and cervical cancer screening. Methods: We conducted a nested case–control study involving a cohort of women in Alberta born between 1994 and 1997 who had at least 1 Pap test between 2012 and 2015. Women with negative cytology results were controls. Women with low-grade (atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion) and high-grade (atypical squamous cells, cannot rule out a high-grade lesion; or high-grade squamous intraepithelial lesion) cervical abnormalities were cases. Exposure status was assigned according to records of HPV vaccination. Odds ratios (ORs) for abnormal cytology results by vaccination status were adjusted for neighbourhood income, laboratory service, rural versus urban residency, and age. Results: The total study population was 10 204. Adjusting for age, vaccinated women had a higher screening rate than unvaccinated women (13.0% v. 11.4%, p vaccination (≥ 3 doses), the adjusted OR for cervical abnormalities was 0.72 (95% confidence interval [CI] 0.63–0.82). For high-grade lesions, the adjusted OR was 0.50 (95% CI 0.30–0.85). With 2-dose HPV vaccination, the adjusted OR for cervical abnormalities was 1.08 (95% CI 0.84–1.38). Interpretation: Quadrivalent HPV vaccination significantly reduced high-grade cervical abnormalities but required 3 doses. Vaccination against HPV was associated with screening uptake. Population-based vaccination and screening programs should work together to optimize cervical cancer prevention. PMID:27378467

  15. The significance in expression of protein p16 in liquid-based thinner cytology to cervical screening%宫颈薄层液基细胞中P16ink4a的表达在宫颈癌筛查中的意义

    Institute of Scientific and Technical Information of China (English)

    韩雪松; 王钫; 徐琳; 许妙玲

    2011-01-01

    Objective To evaluate the significance in expression of protein pl6 which is the product of tumor-suppressing gene P16ink4a in liquid-based cytology to cervical screening and the confidence to be the biological marker of cervical intraepithelial neo-plasia(CIN) .Methods SP immunohistochemistry detection of protein pl6 was performed on 78 specimens remaining from liquid-based cytology which HPV detection was positive ,and compared with the results of biopsy histology .Results In patients with cytolog-ical diagnosis of negative for intraepithelial lesion or malignancy (NILM ) ,atypical squamous cells (ASC ) ,low-grade squamous intraep-ithelial lesion(LSIL) ,and high-grade squamous intraepithelial lesion (HSIL) ,the positive rates of protein pl6 were 0(0/20)、38.9% (14/36),25 .0% (5/2)、85 .0% (17/20),respectively .The positive rate in ASC and HSIL groups was significantly higher than NILM group( ρ<0 .05) .Whereas for LSIL group was not ( ρ>0 .05) .Compared with the results of biopsy histology ,one case was positive of protein pl6 in chronic cervicitis group (2 .70% ,1/36) ,40 cases in CIN (72 .7% ,40 /55 ) ,and 5cases in SCC (100 .0% ,5 / 5 ) .The difference in the positive rates between the first two groups ( ρ<0 .05 ) .Conclusion The abnormal expression of protein pl6 has connected with the development of CIN ,which can become the maker of CIN to help cytology screening .%目的 探讨肿瘤抑制基因P16ink4a的编码产物p16蛋白的表达在宫颈液基薄层细胞学(liquid based cytological test,LCT)筛查中的意义及作为宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)生物标志物的可行性和临床意义.方法 选择96例HPV阳性的宫颈液基薄层细胞学剩余标本,制作液基薄片,利用SP免疫组化法检测LCT标本中p16蛋白,并与组织活检结果进行对照.结果 p16蛋白在宫颈细胞学诊断未见上皮内病变及癌变(NILM)、非典型鳞状上皮细胞(ASC)、低度鳞状上皮内

  16. Diagnosis and analysis of 5 268 cases of cervical lesions by liquid based cytology combined with colposcopy%液基细胞学联合阴道镜检查对5268例宫颈病变的诊断分析

    Institute of Scientific and Technical Information of China (English)

    朱兆霞; 孙颖慧; 辛英

    2016-01-01

    目的:探讨液基薄层细胞学(TCT)技术联合阴道镜检查对宫颈病变的诊断效果。方法:分析TCT结果5268例,并分析阳性者年龄分布特征。结果:5268例妇女中异常细胞学检查结果300例(5.69%),85例经阴道镜多点活检,检出率1.6%。不同年龄段的筛查,以36~45岁为高发年龄,各年龄组比较,差异有统计学意义(P<0.05);TCT检测与病理组织学检查比对检出符合率高,且病理级别越高,符合率越高。ASC-H 符合率66.67%、LSIL 符合率87.50%,HSIL、SCC的符合率100%,经统计学分析,差异有统计学意义(P<0.05)。结论:宫颈癌近年来有年轻化趋势,TCT联合阴道镜检出准确率较高,误诊率低,操作简便。%Objective:To investigate the effect of liquid based cytology test(TCT)technique combined with vaginal cytology in the diagnosis of cervical lesions.Methods:We analyzed the TCT results in 5 268 cases,and the age distribution characteristics of the positive were analyzed.Results:There were 300 cases(5.69% )of abnormal cytology in 5 268 women,85 cases with multiple colposcopic biopsy,and the detection rate was 1.6% .Screening of different ages,taking 36 to 45 years old as the age of high incidence,and there were significant differences among different age groups(P<0.05).TCT examination combined with histopathological examination had a high detection coincidence rate.And the higher the level of pathology,the higher the compliance rate.The coincidence rate of ASC-H was 66.67% and LSIL was 87.50%,the coincidence rate of SCC and HSIL was 100%,which had a significant difference(P<0.05).Conclusion:There is a younger trend of cervical cancer in recent years.The detection accuracy of TCT combined with vaginal cytology is higher.The misdiagnosis rate is low,and the operation is simple.

  17. Detection of Cervical Liquid Based Cytology in the Meaning of the Lotus Pond Town Women Cervical Cancer Screening%宫颈液基细胞学检测在荷塘镇妇女宫颈癌筛查中的意义

    Institute of Scientific and Technical Information of China (English)

    霍喜兰

    2014-01-01

    目的:探讨宫颈液基细胞学检查技术(TCT)在荷塘镇宫颈癌筛查中的应用价值。方法:对1328例本镇及外来务工已婚妇女使用液基细胞学检测(LCT)进行宫颈癌筛查。采用TBS诊断系统选择TCT报告ASCUS以上患者428例行电子阴道镜检并活检病理,将两者结果进行比对分析。结果:宫颈液基细胞学检查1328例中428例阳性,阳性率32.2%。ASCUS 128例,LSIL 86例,ASC-H 90例,HSIL 124例。对TCT检查阳性的428例进行阴道镜病理检查,正常164例,CIN Ⅰ级102例,CIN Ⅱ/Ⅲ级154例,鳞癌8例。宫颈液基细胞学检查结果与组织病理结果符合率,LSIL与CINⅠ级符合率84.3%(86/102),HSIL与CINⅡ/Ⅲ级符合率80.5%(124/154)。结论:基层医院积极开展宫颈癌筛查LCT检测,有助于癌前病变的早发现、早诊断、早治疗。%Objective:To study the cervical liquid based cytology examination technology (TCT) in the lotus pond,the value of cervical cancer screening. Method:The town and migrant workers,1328 cases of married women with a Liquid based cytology test (Liquid-based cytology test,LCT) for cervical cancer screening. Patients with ASCUS by TBS diagnosis system choice TCT report 428 routine electron microscopic examination of the vagina and biopsy pathology,to compare the two results were analyzed. Result:In 1328 patients with cervical liquid based cytology,428 cases of positive,positive rate was 32.2%. ASCUS 128 example, LSIL 86,ASC-H 90,HSIL 124 cases. For TCT examination of 428 patients with colposcope pathologic examination,the result:164 patients with normal,CINⅠgrade 102 cases,CINⅡ/Ⅲlevel of 154 cases squamous carcinoma,8 cases of cervical liquid based cytology examination results and the histopathological results coincidence rate,LSIL and CINⅡ/Ⅲlevel coincidence rate 84.3%(86/102),HSIL and CINⅡ/Ⅲlevel coincidence rate 80.5%(124/154). Conclusion:Basic-level hospital actively carry out cervical cancer

  18. HPV-Based Screening, Triage, Treatment, and Followup Strategies in the Management of Cervical Intraepithelial Neoplasia

    Directory of Open Access Journals (Sweden)

    Oscar Peralta-Zaragoza

    2013-01-01

    Full Text Available Cervical cancer is the second most common cause of death from cancer in women worldwide, and the development of new diagnostic, prognostic, and treatment strategies merits special attention. Many efforts have been made to design new drugs and develop immunotherapy and gene therapy strategies to treat cervical cancer. HPV genotyping has potentially valuable applications in triage of low-grade abnormal cervical cytology, assessment of prognosis and followup of cervical intraepithelial neoplasia, and in treatment strategies for invasive cervical cancer. It is known that during the development of cervical cancer associated with HPV infection, a cascade of abnormal events is induced, including disruption of cellular cycle control, alteration of gene expression, and deregulation of microRNA expression. Thus, the identification and subsequent functional evaluation of host proteins associated with HPV E6 and E7 oncoproteins may provide useful information in understanding cervical carcinogenesis, identifying cervical cancer molecular markers, and developing specific targeting strategies against tumor cells. Therefore, in this paper, we discuss the main diagnostic methods, management strategies, and followup of HPV-associated cervical lesions and review clinical trials applying gene therapy strategies against the development of cervical cancer.

  19. HPV-Based Screening, Triage, Treatment, and Followup Strategies in the Management of Cervical Intraepithelial Neoplasia.

    Science.gov (United States)

    Peralta-Zaragoza, Oscar; Deas, Jessica; Gómez-Cerón, Claudia; García-Suastegui, Wendy Argelia; Fierros-Zárate, Geny Del Socorro; Jacobo-Herrera, Nadia Judith

    2013-01-01

    Cervical cancer is the second most common cause of death from cancer in women worldwide, and the development of new diagnostic, prognostic, and treatment strategies merits special attention. Many efforts have been made to design new drugs and develop immunotherapy and gene therapy strategies to treat cervical cancer. HPV genotyping has potentially valuable applications in triage of low-grade abnormal cervical cytology, assessment of prognosis and followup of cervical intraepithelial neoplasia, and in treatment strategies for invasive cervical cancer. It is known that during the development of cervical cancer associated with HPV infection, a cascade of abnormal events is induced, including disruption of cellular cycle control, alteration of gene expression, and deregulation of microRNA expression. Thus, the identification and subsequent functional evaluation of host proteins associated with HPV E6 and E7 oncoproteins may provide useful information in understanding cervical carcinogenesis, identifying cervical cancer molecular markers, and developing specific targeting strategies against tumor cells. Therefore, in this paper, we discuss the main diagnostic methods, management strategies, and followup of HPV-associated cervical lesions and review clinical trials applying gene therapy strategies against the development of cervical cancer. PMID:23690785

  20. The study of thinprep cytology test combined truscreen in early diagnosis cervical precancerous lesions%膜式薄层液基细胞学检查联合初善仪在子宫颈癌前病变诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    张小鹏; 罗新; 王晓玉

    2012-01-01

    目的 探讨膜式薄层液基细胞学检查(thinprep cytologic test,TCT)联合初善仪(truscreen,TS)在子宫颈癌前病变诊断中的应用价值.方法 对30例阴道分泌物异常的患者同时行TCT和TS检查,再行阴道镜检查及组织活检,以组织病理学检查结果作为诊断的金标准.比较2种检查方法单独及联合使用与组织病理学检查结果的吻合程度.结果 30例患者中有25例病理学诊断为子宫颈上皮内瘤变1级及以上病变,为真阳性.TCT测试细胞学意义不明的不典型鳞状上皮细胞以上的阳性19例(63.3%),假阴性6例,漏诊率20.0%,真阳性16例,敏感度84.2%.TS测试阳性18例(60.0%),假阴性7例,漏诊率23.3%,真阳性14例,敏感度77.8%.TCT联合TS测试阳性27例(90.0%),假阴性1例,漏诊率3.3%,真阳性24例,敏感度88.9%.TCT联合TS检测子宫颈癌前病变的阳性率与病理学检查的阳性率分别为90.0%和83.3%,差异无统计学意义(P>0.05).结论 TCT联合TS检查诊断子宫颈癌前病变的准确性明显优于任一单项检查,其漏诊率显著下降.%Objective: To investigate the application value of thinprep cytology test(TCT) combined truscreen(TS) in early diagnosis cervical precancerous lesions. Methods:Thirty patients with abnormal vaginal secretions were detected under TCT and TS,and then vaginal tissue biopsies were tested under colposcopy. The results of pathological examination acted as the gold standard of diagnosis. The match degrees of single and combined using methods and histopathological examination results were compared. Results: The results of histopathological examination of 25 cases were cervical intraepithelial neoplasia grade 1 or above. Nineteen cases(63. 3% ) were ASCUS cytology positive,6 cases were false negative,16 cases were true postive,the rate of fail to diagnosis was 20.0% ,and the sensitiveness degree was 84. 2% under TCT. Compared with TCT results,the data were 18 cases(60. 0% ) ,7 cases, 14 cases,23. 3% and

  1. The Comparative Analysis of Liquid Based Cytology Diagnosis and Histological Diagnosis in 83 Cases of Cervical Squamous Cell Carcinoma%宫颈癌患者液基细胞学与组织学诊断的比较分析

    Institute of Scientific and Technical Information of China (English)

    仇波; 彭悦; 邓亚平; 刘志红; 曾亮

    2012-01-01

    目的:通过对液基细胞学检测(TCT)与组织学活检的比较分析,探讨TCT出现漏诊的原因.方法:收集83例宫颈癌患者的宫颈活检组织蜡块和宫颈脱落细胞标本,组织蜡块行常规病理切片检查,脱落细胞标本进行TCT检测.结果:和组织病理学诊断结果比较,这些患者的TCT诊断漏诊18例.进行过抗HPV治疗的宫颈癌患者,其TCT漏诊率明显高于未治疗者(P<0.05).结论:TCT在宫颈癌筛查中存在一定的漏诊,其产生的原因与癌细胞分化程度、宫颈糜烂程度、非诊断细胞的干扰、不当的采集方法及抗HPV治疗有关.%Objective: To explore the causes of the errors of liquid based cytology test, through a comparative analysis of liquid based cytology test and histology of biopsy. Methods: The paraffin blocks of cervical biopsies and the cervical cytology specimens of 83 cases of cervical carcinoma were collected, the paraffin blocks were for routine HE staining and histological diagnosis, the cytology specimens were dectected with liquid based cytology test and diagnosis. Results: Compared to histological diagnosis, the diagnosis in the liquid based cytology of these patients showed that 18 cases were missed diagnosed. The rate of misdiagosis of these patients whot-had been regularly anti-HPV treated was significantly higher than that of the others (P<0.05). Conclusion: There were some errors in liquid based cytology test in cervical cancer screening, the reasons associated with the cell differentiation ,the degree of cervical erosion, the interference from non-diagnostic cells, the improper collection method and that the patients had been regularly anti-HPV treated.

  2. The APTIMA HPV assay versus the Hybrid Capture 2 test in triage of women with ASC-US or LSIL cervical cytology: a meta-analysis of the diagnostic accuracy.

    Science.gov (United States)

    Arbyn, Marc; Roelens, Jolien; Cuschieri, Kate; Cuzick, Jack; Szarewski, Ann; Ratnam, Sam; Reuschenbach, Miriam; Belinson, Suzanne; Belinson, Jerome L; Monsonego, Joseph

    2013-01-01

    Testing for DNA of 13 high-risk HPV types with the Hybrid Capture 2 (HC2) test has consistently been shown to perform better in triage of women with cervical cytology results showing atypical squamous cells of undetermined significance (ASC-US) but often not in triage of low-grade squamous intraepithelial lesions (LSIL) detected in cervical cancer screening. In a meta-analysis, we compared the accuracy of the APTIMA HPV test, which identifies RNA of 14 high-risk HPV types, to HC2 for the triage of women with ASC-US or LSIL. Literature search-targeted studies where the accuracy of APTIMA HPV and HC2 for detection of underlying CIN2/3+ was assessed concomitantly including verification of all cases of ASC-US and LSIL. HSROC (Hierarchical Summary ROC) curve regression was used to compute the pooled absolute and relative sensitivity and specificity. Eight studies, comprising 1,839 ASC-US and 1,887 LSIL cases, were retrieved. The pooled sensitivity and specificity of APTIMA to triage ASC-US to detect underlying CIN3 or worse was 96.2% (95% CI = 91.7-98.3%) and 54.9% (95% CI = 43.5-65.9%), respectively. APTIMA and HC2 showed similar pooled sensitivity; however, the specificity of the former was significantly higher (ratio: 1.19; 95% CI = 1.08-1.31 for CIN2+). The pooled sensitivity and specificity of APTIMA to triage LSIL were 96.7% (95% CI = 91.4-98.9%) and 38.7% (95% CI = 30.5-47.6%) for CIN3+. APTIMA was as sensitive as HC2 but more specific (ratio: 1.35; 95% CI = 1.11-1.66). Results were similar for detection of CIN2 or worse. In both triage of ASC-US and LSIL, APTIMA is as sensitive but more specific than HC2 for detecting cervical precancer.

  3. 液基细胞学及HPV分型检测在宫颈病变筛查中的应用%Thinprep Cytology Test and HPV Genotyping in Screening of Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    焦凤娟; 韩洁

    2013-01-01

    Objective: To investigate the clinical significance of thinprep cytology test (TCT) and HPV genotyping in cervical le-sions. Methods: TCT and HPV genotyping were done for 390 cases of cervical lesions, and did the biopsy for who was ≥ASCUS. Results: In the 390 cases, the positive rates of TCT and HPV were 26.41% and 28.72%; the rate of high-risk type of HPV in 103 cas-es of TCT-positive patients was 63.11%, and the biopsy showed that there were inflammation 46 cases, CIN I 24 cases, CIN II 12 cases, CIN III 18 cases and cervical cancer 3 cases. Conclusion: TCT and HPV genotyping can improve the detection rate of cervical cancer and precancerous lesions, it has important significance in early diagnosis and prevention for cervical cancer.%  目的:探讨液基细胞学(TCT)联合HPV 分型检测在宫颈病变筛查中的临床意义.方法:对390例宫颈病变患者行TCT 检查和 HPV 检测,对≥ASCUS 患者行组织活检.结果:390例患者中 TCT 和 HPV 阳性率分别为26.41%、28.72%;103例TCT阳性患者中高危型HPV阳性率为63.11%,组织活检结果为炎症46例、CIN I 24例、CIN II 12例、CIN III 18例和宫颈癌3例.结论:TCT联合HPV检测可提高对宫颈癌及癌前病变的检出率,对宫颈癌的早期诊断和防治有重要临床意义.

  4. 液基细胞学联合初善仪检测在宫颈癌筛查中的应用%Value of TruScreen combined with Thinprep cytology test in screening of cervical lesions

    Institute of Scientific and Technical Information of China (English)

    杜欣欣; 赛晓勇; 刘爱军; 张云; 付晓宇; 李江华; 孟元光

    2015-01-01

    目的:探讨液基细胞学(TCT)与初善仪(TS)在宫颈病变筛查中的联合应用价值。方法选择2013年12月~2014年11月就诊于解放军总医院妇科门诊阴道镜室的患者218例,所有患者均给予TCT及TS检查,并根据TCT及TS检查结果进行分组,结合病理学结果分析两种检测方法细胞学诊断价值的差异。结果TCT结果显示,218例患者中,不能明确意义的非典型鳞状细胞(ASC-US)76例,非典型鳞状细胞不除上皮内高度病变(ASC-H)43例,低度鳞状上皮内病变(LSIL)58例,高度鳞状上皮内病变(HSIL)41例;TS检测异常者94例。病理结果正常者106例,LSIL/CIN 141例,HSIL/CIN 2~371例。病理结果正常患者中,细胞学ASC-H与LSIL的检出率分别为53.5%和56.9%;低级别病变患者中,ASC-US和LSIL的检出率显著高于ASC-H及HSIL组(P=0.005);高级别病变患者中,ASC-H及HSIL的检出率显著高于ASC-US和LSIL(P=0.004)。经TS分层后,TS阴性而病理结果正常的患者中,TCT各组的检出率均提高;TS阳性而病理低级别病变患者中,ASC-US与LSIL的检出率升高,ASC-H与HSIL呈下降趋势,高级别病变患者中,TBS分级四组的检出率均升高。结论联合应用TS可以减少阴道镜门诊的过度转诊并能够更灵敏地发现高级别病变患者。%Objective To investigate the value of TruScreen (TS) combined with Thinprep cytology test in screening of cervical lesions. Methods 218 patients from December 2013 to November 2014 in Outpatient Vaginoscopy Room of PLA General Hospital, all patients were given the detection of TCT and TS, they were divided into different group ac-cording the detection results of TCT and TS, and diagnostic value of TCT and TS were evaluated by pathology result as a golden standard. Results The results of TCT showed that, there were 76 cases of ASC-US, 43 cases of ASC-H, 58 cases of LSIL and 41 cases of HSIL in total of 218 patients; TS showed that 94 cases were abnormal. Pathological re

  5. 宫颈液基细胞学检查、HPV分型、LEEP环状电切术在宫颈疾病中的诊治意义%Diagnostic and therapeutic significance of thinprep cytologic test, HPV genotyping and loop electrosurgical excision procedure for cervical diseases

    Institute of Scientific and Technical Information of China (English)

    谷新; 邓雷

    2012-01-01

    Objective To investigate the diagnostic and therapeutic significance and clinical value of thinprep cytologic test, HPV genotyping and loop electrosurgical excision procedure for cervical diseases. Methods From January to December 2010 in Enshi center hospital, 4 613 patients were taken gynecological examination and thinprep cytologic test, 336 cases with the positivity of thinprep cytologic test were taken electronic colposcopy and colposcopic cervical biopsy, diagnosed 125 cases of cervical intraepithelial neoplasia-like lesions and cervical cancer, they were taken HPV genotyping. cervical severe erosion and repeated physical therapy chronic cervicitis of CIN Ⅱ and some CIN I , and early diagnosis of cervical cancer conization were treated with loop electrosurgical excision procedure, the surgical removal of tissue were taken pathological examination. Results The diagnosis rate was 98.6% for cervical liquid-based brush cytology proposed line cervical biopsy of electronic colposcopy and colposcopy, HPV genotyping check, HPV genotyping check positive 125 cases and the the loop electrosurgical excision procedure after excision pathological examination, no case of intraoperative bleeding in the surgery, 5 cases with cervical wound after the scab off bleeding, and no case of postoperative infection. Conclusion Electronic colposcopy, thinprep cytologic test, HPV genotyping and cervical biopsy has important diagnostic value for cervical precancerous lesions; the loop electrosurgical excision procedure in the treatment of cervical disease has small risk of surgery, and simple operation, less bleeding and high success rate, loop electrosurgical excision has important clinical value in the diagnosis and treatment of cervical disease.%目的 探讨宫颈液基细胞学检查、HPV分型、LEEP环状电切术在宫颈疾病中的诊治意义及临床价值.方法 对2010年1~12月在恩施州中心医院就诊的4 613例患者行妇检、宫颈液基细胞学刷片检查,

  6. 酸性磷酸酶筛查宫颈异常细胞的临床实验研究%Clinical studies of acid phosphatase screening cervical abnormal cells

    Institute of Scientific and Technical Information of China (English)

    李洪言; 李嘉; 魏兆莲; 赵卫东; 雷蕾; 周平; 吴娟; 许孝凤; 王影; 胡卫平

    2012-01-01

    分别采用酸性磷酸酶(CAP)和巴氏染色对208例妇科门诊患者的宫颈脱落细胞进行染色.CAP筛查宫颈异常细胞的检出率明显高于巴氏染色.%208 cases of gynecological outpatients' cervical exfoliated cells were stained by acid phosphatase and Papanicolaou dye, to investigate the efficacy and safety of screening the abnormal cervical cells by acid phosphatase. The results indicated that the check rates of screening abnormal cervical cells by acid phosphatase were significantly higher than those of the conventional Papanicolaou stain.

  7. Analysis of cervical smears in a Muslim population

    International Nuclear Information System (INIS)

    Screening for the cancer of the cervix remains a neglected health care issue in Pakistan. To provide baseline data for future efforts to improve screening, we conducted a retrospective analysis of cervical smears taken in the obstetrics and gynaecological clinics of the Agha Khan University Hospital, Karachi, Pakistan. We collected data on cervical smear cytology for cervical smears taken from January 1, 1990 to December 31, 1996. We assessed risk factors for dysplasia, including age, age at first marriage, and number of pregnancies. The overall prevalence of abnormal smears in our study was 0.5%. Of 20,995 cervical smears, showed non-specific inflammation, 7302 (34.8%) were reported as normal, 809 (3.85%) showed monillial infection, 148 (0.71%) showed atypia, 105 (0.5%) had dyplastic cytology and 52 (0.25%) samples were inadequate. The highest incidence of dysplastic smears was seen in the age group 35 to 44 years. Of 105 patients with dysplasia 12 were pregnant, and all were asymptomatic. The low prevalence of abnormal smears, compared with the data from Western populations, could be due to the internet bias of health awareness in the women who attended our hospital. The results of this study may serve as baseline for for future comparisons. A large community-based study may establish the exact prevalence of malignant and premalignant lesions so as to plan for future screening. (author)

  8. Liquid-based Cytology with Colposcopy in the Clinical Diagnostic Application of Cervical Lesions%液基细胞学联合阴道镜检诊断宫颈病变

    Institute of Scientific and Technical Information of China (English)

    陈宁静; 涂干卿

    2010-01-01

    目的 研究液基薄层细胞学(TCT)检查联合阴道镜检对宫颈病变诊断的价值.方法 选择1 407例患者进行TCT、阴道镜检查和活组织病理检查.结果 281例单独行阴道镜检和699例单独行TCT检查提示活检的,分别有39.50%和42.63%属CIN Ⅰ级以上病变;427例TCT检查结合阴道镜检提示活检的,有86.42%属CIN Ⅰ级以上病变.结论 TCT检查结合阴道镜检定位活检可提高宫颈病变的准确诊断率.%Objective Studying the clinical diagnostic value of liquid-based thin-layer cytology(TCT)combined colpoacopic examination for cervical lesions.Methods In May 2006~August 2007 choose the 1407 patients coming the hospital who was done cytology examination(TCT)and coiposcopy examination and biopsy.Results Among 281 cases of eolposcopy prompt biopsy 39.50% of them suffered from CIN Ⅰ.So did 42.63 % of 699 cases of TCT examinations.427 cases of TCT combined Colposcopy were prompted they need to do biopsy.86.42% of them suffered from CIN Ⅰ or above lesions.Conclusion TCT combination of colposcopy and endoscopic biopsy can increase the rate of accurate diagnosis of cervicallesions.

  9. Conjunctival impression cytology in contact lens wearers.

    Directory of Open Access Journals (Sweden)

    Simon Priya

    2002-01-01

    Full Text Available PURPOSE: To evaluate the cytological changes in conjunctiva following regular contact lens wear and to determine the correlation, if any, between severity of cytological alteration and symptoms related to contact lens wear. METHODS: One hundred eyes (50 normal asymptomatic subjects who served as a control group were studied by conjunctival impression cytology (CIC. These subjects were fitted with rigid gas permeable (RGP or soft contact lenses and were followed up at the end of 3 and 6 months. At each follow-up visit the CIC was repeated. A filter paper with the impression specimen was stained with periodic acid schiff (PAS and haematoxylin stain to study goblet cell loss. Papanicolaou stain was done to study squamous metaplasia. The cytological changes were graded using the system described by Natadisastra et al. RESULTS: Severity of cytological changes increased with the duration of contact lens wear (P = 0.00001. At the end of 6 months, 60% of symptomatic eyes wearing soft contact lens and RGP lens showed abnormal CIC changes. None of the asymptomatic RGP contact lens wearing eyes showed abnormal CIC changes whereas 33.4% of the asymptomatic soft contact lens wearing eyes showed abnormal CIC changes (P = 0.033. Epithelial changes occurred within 3-6 months of contact lens fitting. CONCLUSION: Severity of cytological changes increased with duration of lens wear (P = 0.00001. Prevalence and severity of cytological alteration is more in symptomatic contact lens wearers. Soft contact lens wearers although asymptomatic showed severe CIC changes.

  10. Evaluation of colposcopy vs cytology as secondary test to triage women found positive on visual inspection test

    Directory of Open Access Journals (Sweden)

    S A Pimple

    2010-01-01

    Full Text Available Background: Colposcopic evaluation and guided biopsy is an important diagnostic step and standard of management for abnormal cytology smears in developed countries. Aim: The present study evaluates the performance of colposcopy vs conventional cytology in estimating the presence and grade of cervical disease against the reference standard of histopathology as a secondary test modality to triage women found positive on primary screening by visual inspection with 5% acetic acid (VIA. Settings and Design: Colposcopy and directed biopsy were performed after primary screening for cervical cancer in an urban hospital cancer screening clinic between January 2000 and June 2005, in Mumbai, India. Materials and Methods: Healthy women (8863 in the age group of 35-65 years participated in the cervical cancer early detection program in the hospital and community cancer screening clinics in Mumbai. Women found positive on the primary screening test by VIA underwent diagnostic evaluation by Pap smear cytology and colposcopy evaluation with directed biopsies. Statistical Analysis: Accuracy parameters and their 95% confidence intervals were calculated using 2 Χ 2 tables and standard formulae. Results: The test range of sensitivity of colposcopy for the detection of histologically confirmed cervical intraepithelial neoplasia grade 1+ (CIN1+ or CIN2+ was 58.0-74.7% and that of specificity was 57.5-92.9%. The sensitivity and specificity of cytology to detect CIN2+ was 57.4% and 99.4%, respectively. Conclusion: Colposcopy is a good sensitive test for the detection of CIN and can be considered as a secondary testing tool to triage women found positive on VIA.

  11. 液基薄层细胞学检查配合阴道镜检查对子宫颈病变的诊断价值%The diagnostic value of thinprep cytology combined with colposcopy for cervical lesions

    Institute of Scientific and Technical Information of China (English)

    白杰

    2014-01-01

    Objective To investigate the diagnostic value of ThinPrep cytology(TCT) combined with colposcopy for cervical lesions.Methods 980 patients with suspected cervical lesions were selected and received TCT combined with colposcopy examination.Among the 980 patients,102 patients with positive smear were referred for colposcopy and were diagnosed by colposcopy biopsy.Results Of 102 patients with positive smear,the detection rates of the atypical squamous cells (ASC),low-grade squamous intraepithelial lesions (LSIL),cervical intraepithelial neoplasia (HSIL) and atypical glandular cells (AGC) were 6.1%,0.6%,2.1% and 1.5%,respectively.Compared with the colposcopy biopsy for cytology samples,the test results were as follows:LSIL was 72.4% (21/29),HSIL was 93.8% (15/16).Conclusion The method of TCT could increase the screening rate of positive diagnosis of cervical lesions and for the highly suspected patients,the combination of vaginal colposcopy and biopsy could further improve the accuracy of diagnosis,in order to provide scientific solutions for clinical treatment.%目的 探讨液基薄层细胞学检查(TCT)联合阴道镜检查对子宫颈病变的临床诊断应用价值.方法 选取疑似为宫颈病变的患者980例,采用TCT联合阴道镜检查,其中,送检样本阳性涂片患者102例,均行阴道镜检查,于阴道镜下取组织活检.结果 980例患者的102例阳性患者中,不典型鳞状上皮细胞(ASC)、低度鳞状上皮内病变(LSIL)、宫颈癌前病变(HSIL)及不典型腺细胞(AGC)的检出率分别为6.1%、0.6%、2.1%和1.5%;对细胞学阳性样本进行阴道镜组织活检,两种检测结果比较:LSIL为72.4% (21/29),HSIL为93.8% (15/16).结论 TCT筛查可增加宫颈病变阳性诊断率,对于高度疑似患者配合阴道镜检查及阴道组织活检可以进一步提高诊断的准确度,以便为临床治疗提供科学的方案.

  12. The investigation on the cervical lesions in the combined use of cytology and the significance of hu-man papilloma virus detection%探讨子宫颈病变中联合应用细胞学及人类乳头状瘤病毒检测的意义

    Institute of Scientific and Technical Information of China (English)

    刘禄; 杨娜; 杨槐; 蒲泽宴; 刘方久; 张薇珊; 崔丽娟; 罗启翅

    2016-01-01

    目的:研究子宫颈病变诊断中联合细胞学和人类乳头状瘤病毒检测对病理诊断的作用。方法:收集遂宁市中心医院病理科子宫颈活组织检查标本876例,分别进行细胞学检查及人类乳头状瘤病毒检测,采用统计学软件进行数据分析。结果:31~50岁为高危型 HPV 感染高发年龄,分别占26.7%和45.7%。子宫颈鳞状细胞癌在41~50岁年龄组占43.7%。结论:子宫颈脱落细胞学及高危型 HPV 检测在子宫颈癌筛查及诊断中具有重要作用,随着我国经济社会的发展,国家应加大筛查的宣传力度,子宫颈脱落细胞学及高危型 HPV 检测可以明显降低子宫颈癌的发病率。%Objective:To research joint cytological diagnosis of cervical lesions and human papilloma virus detection of pathological diagnosis. Methods:Collection of suining city central hospital,876 cases of cervical biopsy specimens,respectively for cytologic examination and detection of human papilloma virus using statistical software for data analysis. Results:31 to 50 years old in high - risk type HPV infection high - risk age,respectively,26. 7% ,45. 7% . Cervical squamous cell carcinoma (43. 7% )as the highest in group C. Conclusion:Type cervical exfoliated cytology and high - risk HPV detection in cervical cancer screening and diagnosis plays an important role,along with the development of China's economic and social,countries should step up the propaganda screening,cervical exfoliated cytology and high - risk type HPV testing can significantly reduce the incidence of cervical cancer.

  13. Cervical screening in England: the past, present, and future.

    Science.gov (United States)

    Albrow, Rebecca; Kitchener, Henry; Gupta, Nalini; Desai, Mina

    2012-04-25

    Cervical screening in England commenced in a disorganized fashion in 1964. The flaws of this approach became apparent in the mid-1980s and led to the inception of the National Health Service Cervical Screening Programme (NHSCSP). The main features of this program are its population-based registry, accessibility to all women within the screening age range, its systematic process of call and recall, national coordination, and quality assurance. Its success is in part based on its ability to evolve as evidence necessitates, and throughout the period of 2000-2010, it embarked upon a series of developments involving liquid-based cytology, which also provided the means to conduct reflex high-risk human papillomavirus (HR-HPV) testing and the potential to automate the screening process. As a result of evidence acquired since 2000, the NHSCSP is currently based on a system of primary cytology with HPV triage for low-grade abnormalities combined with cytology plus a HR-HPV "test of cure" for women who have received treatment for cervical intraepithelial neoplasia. Future challenges for the program will involve finding solutions to increasing screening uptake among women HPV primary screening. PMID:22367883

  14. 不能明确意义的不典型鳞状细胞伴DNA倍体异常在宫预早期病变筛查中的意义%Diagnostic implications of atypical squamous cells of unknown significance with abnormal DNA ploidy for early cervical lesions

    Institute of Scientific and Technical Information of China (English)

    梅金红; 徐姗; 韩永良; 涂轶; 熊一峰; 余燕青

    2013-01-01

    Objective To investigate the clinical significance of atypical squamous cells of unknown significance (ASCUS) with abnormal DNA ploidy in the early diagnosis of cervical lesions.Methods Eight thousand four hundred and forty-eight patients were included in this study and all had DNA quantitative analysis and cervical liquid-based cytology.Among 1041 cases with DNA aneuploidy and/or abnormal cervical liquid-based cytology and additional cervical biopsy,histological review was performed in 247 ASCUS cases with abnormal DNA ploidy.Results (1) Among 8448 cases,7877 were normal or benign,426 were ASCUS,45 were ASC-H,55 were LSIL and 22 were HSIL by TBS diagnosis.The presence of 1-2 abnormal DNA ploidy cells was detected in 15.3% (65/426) of ASCUS,11.1% (5/45) of ASC-H,9.1% (5/55) of LSIL,and 0 (0/22) of HSIL.The presence of ≥ 3 abnormal DNA ploidy cells was detected in 39.0% (166/426) of ASCUS,75.6% (34/45) of ASC-H,76.4% (42/55) of LSIL,and 95.5% (21/22) of HSIL.(2) A total of 67 cases of CIN 2,CIN 3 or cancers were found in 247 patients with ASCUS by colposcopy biopsies,of which 13.9% (5/36) had 1-2 abnormal DNA ploidy cells,45.5% (56/123) had ≥3 abnormal DNA ploidy cells and 6.8% (6/88) had normal DNA polidy.ASCUS with 1-2 abnormal DNA ploidy cells and with ≥ 3 abnormal DNA ploidy cells were compared.The difference was statistically significant (x2 =11.79,P <0.01).But the difference between ASCUS with 1-2 abnormal DNA ploidy cells and normal DNA ploidy had no statistical significance (P > 0.05).Conclusions ASCUS with ≥3 abnormal DNA ploidy cells has higher risk for developing CIN 2,CIN 3 or invasive carcinoma.The application of DNA quantitative analysis and cervical liquid-based cytology test can help in guiding clinical follow-up and treatment options in patients with ASCUS.%目的 通过DNA定量分析与薄层液基细胞学检查,探讨不能明确意义的不典型鳞状细胞(ASCUS)伴DNA倍体异常在宫颈早期

  15. 液基细胞学联合人乳头瘤病毒检查在妊娠期宫颈病变筛查中的作用%Effect of liquid-based cytology combined with human papillomavirus test in screening of cervical lesions during pregnancy

    Institute of Scientific and Technical Information of China (English)

    关嵩青; 叶菲

    2012-01-01

    目的:探讨液基细胞学(TCT)联合人乳头瘤病毒(HPV)检查在妊娠期宫颈病变筛查中的价值.方法:采用TCT联合HPV检查的方法,对在深圳市第二人民医院行产前检查的500例妊娠期妇女进行宫颈病变筛查,以宫颈组织病理学检查结果作为确诊的金标准.结果:500例中,高危HPV阳性检出率为28.00%,TCT阳性检出率为8.40%,HPV与TCT同时阳性与阴道镜活检病理结果的阳性符合率为89.29%.结论:产前检查是进行宫颈病变筛查的良好时机,TCT联合HPV检查与阴道镜活检病理结果的阳性符合率高,用于妊娠期宫颈病变筛查是安全和有效的.%Objective: To explore the value of liquid — based cytology combined with human papillomavirus (HPV) test in screening of cervical lesions during pregnancy. Methods: Liquid-based cytology combined with HPV test was used to carry out screening of cervical lesions in 500 pregnant women in the hospital, the pathological result of cervical tissue was designed as gold standard of definite diagnosis. Results: Among 500 pregnant women, the detection rate of high risk HPV was 28. 00% , and the positive detection rate of liquid-based cytology was 8. 40% , the positive coincidence rate between positive HPV test, liquid-based cytology and pathological result after colposcopic biopsy was 89. 29%. Conclusion: Prenatal examination is a good opportunity for screening of cervical lesions; the positive coincidence rate between liquid-based cytology combined with HPV test and pathological result after colposcopic biopsy was high; the method is safe and effective for screening of cervical lesions during pregnancy.

  16. Performance of Implementing Guideline Driven Cervical Cancer Screening Measures in an Inner City Hospital System

    Science.gov (United States)

    Wieland, Daryl L.; Reimers, Laura L.; Wu, Eijean; Nathan, Lisa M.; Gruenberg, Tammy; Abadi, Maria; Einstein, Mark H.

    2013-01-01

    Objective In 2006, the American Society for Colposcopy and Cervical Pathology (ASCCP) updated evidence based guidelines recommending screening intervals for women with abnormal cervical cytology. In our low-income inner city population, we sought to improve performance by uniformly applying the guidelines to all patients. We report the prospective performance of a comprehensive tracking, evidence-based algorithmically driven call-back and appointment scheduling system for cervical cancer screening in a resource-limited inner city population. Materials and Methods Outreach efforts were formalized with algorithm-based protocols for triage to colposcopy, with universal adherence to evidence-based guidelines. During implementation from August 2006 through July 2008, we prospectively tracked performance using the electronic medical record with administrative and pathology reports to determine performance variables such as the total number of Pap tests, colposcopy visits, and the distribution of abnormal cytology and histology results, including all CIN 2,3 diagnoses. Results 86,257 gynecologic visits and 41,527 Pap tests were performed system-wide during this period of widespread and uniform implementation of standard cervical cancer screening guidelines. The number of Pap tests performed per month varied little. The incidence of CIN 1 significantly decreased from 117/171 (68.4%) the first tracked month to 52/95 (54.7%) the last tracked month (p=0.04). The monthly incidence rate of CIN 2,3, including incident cervical cancers did not change. The total number of colposcopy visits declined, resulting in a 50% decrease in costs related to colposcopy services and approximately a 12% decrease in costs related to excisional biopsies. Conclusions Adherence to cervical cancer screening guidelines reduced the number of unnecessary colposcopies without increasing numbers of potentially missed CIN 2,3 lesions, including cervical cancer. Uniform implementation of administrative

  17. Diffusion tensor magnetic resonance imaging may show abnormalities in the normal-appearing cervical spinal cord from patients with multiple sclerosis

    Directory of Open Access Journals (Sweden)

    Fernanda Miraldi

    2013-09-01

    Full Text Available Objective This study aims to evaluate “in vivo” the integrity of the normal-appearing spinal cord (NASC in patients with multiple sclerosis (MS compared to controls, using diffusion tensor MR imaging. Methods We studied 32 patients with MS and 17 without any neurologic disorder. Fractional anisotropy (FA, axial diffusivity (AD, radial diffusivity (RD and mean diffusivity (MD were calculated within regions of interest at C2 and C7 levels in the four columns of the spinal cord. Results At C2, FA value was decreased in MS patients. Besides, RD value was higher in MS than in controls. At C7, MD values were increased in MS. Conclusion The NASC in the right column of the cervical spinal cord showed abnormal FA, RD and MD values, which is possibly related to demyelination, since the FA abnormality was related to the RD and not to the AD.

  18. Surgical treatment of lower cervical injuries combined with cervical congenital abnormalities%合并颈椎先天性畸形的下颈椎损伤的治疗策略

    Institute of Scientific and Technical Information of China (English)

    谢宁; 倪斌; 叶晓健; 陈德玉; 肖建如; 袁文

    2008-01-01

    目的 探讨合并颈椎先天性畸形的下颈椎损伤的治疗策略. 方法 自1998年至2005年收治合并颈椎先天性畸形的下颈椎损伤患者11例,损伤类型包括急性C3/4椎间盘突出症1例,颈椎过伸伤7例,C5骨折1例,C4/5、C5/6脱位各1例.合并的颈椎先天性畸形包括Klippel-Feil综合征2例,颅底凹陷2例,颅底扁平5例,寰椎枕骨化9例,Chiari畸形1例.Frankel分级为A级1例,B级2例,c级5例,D级3例.合并Klippel-Feil综合征者实施了颈前路单间隙减压取髂骨植骨融合带锁钢板固定加后路短节段减压侧块螺钉固定术.1例颈椎过伸伤合并Chiari畸形实施了颈椎后路减压加枕大孔减压枕颈融合后路固定术.余患者依致压物范围和椎管狭窄程度分别实施了颈椎前路减压植骨钢板固定术或颈椎后路减压侧块螺钉固定术. 结果 所有患者获得4个月~6年(平均22个月)随访.术后3个月Frankel分级为B级1例,C级2例,D级3例,E级5例,平均提高1~2级.植骨平均于术后3个月愈合,未出现内置物相关并发症.随访期内未出现颈椎不稳以及颈椎畸形相关的临床症状. 结论 明确没有上颈椎损伤的合并颈椎先天性畸形的下颈椎损伤可以只处理下颈椎损伤,所合并的颈椎畸形根据不同类型区别考虑.对于合并颈椎畸形的下颈椎损伤没有必要为了防止颈椎畸形远期继发的神经症状而融合上颈椎或其他节段.%Objective To discuss strategies of treating lower cervical injuries combined with cervical congenital abnormalities. Methods Eleven cases of lower cervical injuries combined with cervical congenital abnormalities were treated from 1998 to 2005. The injuries included 1 case of C3/4 disc herniation, 7 cases of whiplash injury, 1 case of C5 vertebral body fracture, and 2 cases of C4/5 and C5/6 dislocation.Cervical abnormalities included Klippel-Feil syndrome (2), basilar impression (2), platybasia (5), occipitalization of atlas (9

  19. Liquid Based Cytology and Colposcopy in the Screening of Cervical Cancer and Precancerous Lesions%宫颈液基细胞学及阴道镜在宫颈癌及癌前病变中的筛查作用

    Institute of Scientific and Technical Information of China (English)

    王清; 胡霞

    2015-01-01

    目的:分析液基细胞学和阴道镜在宫颈癌及癌前病变中的筛查作用,评估液基细胞学和阴道镜在宫颈癌及癌前病变筛查中的临床应用价值。方法回顾性分析2013年5月-2014年8月接受宫颈癌筛查的500例女性的临床资料,所有体检者都接受阴道镜检查和液基细胞学检查。结果阴道镜检测假阳性患者有11例,阴道镜检测的特异性为97.39%(412/423),漏诊率为1.00%(5/500);液基细胞学诊断的假阳性患者有15例,由检测结果可知液基细胞学检测宫颈癌的检测特异性为96.45%(408/423),漏诊率为6.40%(32/500)。结论液基细胞学和阴道镜在宫颈癌及癌前病变筛查中具有临床应用价值,阴道镜对宫颈癌及癌前病变的检出率较液基细胞学高。%Objective To study the application value of liquid based cytology and colposcopy in the screening of cervical cancer and precancerous lesions .Methods Retrospective analysis of clinical data of 500 female patients who underwent cervical cancer screening were conducted .All subjects received colposcopy and liquid based cytology .Results Colposcopy results showed that false positive were 11 cases,the specificity of colposcopy was 97.39%(412 /423),the rate of missed diagnosis was 1.00%(5 /500);liquid based cytology diagnosis showed that false positive were 15 cases,specificity was 96.45%(408/423),the rate of missed diagnosis was 6.40%(32 /500).Conclusion Liquid based cytology and colposcopy has clinical application value in the screening of cervical cancer and precancerous lesions ,Colposcopy for cervical cancer and precancerous lesions has higher detec -tion rate compared with liquid based cytology .

  20. Application of human papillomavirus genotyping detection and thinprep cytology test in screening of cervical lesions%HPV分型检测和TCT检查在宫颈病变筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    卜祥茂; 徐莉莉; 陈艳萍; 宋卫青

    2014-01-01

    Objective:To evaluate the role of human papillomavirus(HPV) genotyping detection and thinprep cytology test(TCT )for screening cervical lesions.Methods:A retrospective analysis 1128 cases of cervical cytology specimens from September 2012 to September 2013 using HPV、TCT and cervical biopsy.Results: In 1128 cases of specimens, the positive rate for HPV infection was 64.4%(726/1128) and there were 21 kinds of genotypes, high-risk subtype 16 kinds, low-risk types 5 kinds. High-risk subtype infection were 16 (25.0%), 58 (12.1%), 52(10.2%) and low-risk subtype main was HPV 61(3.4%), 11(1.9%). Single subtype was detected in 555 cases,double or multiple 171 cases, and quadruple infection was also can be detected. TCT ≥ASC-US was 87.8%(990/1128). In ASCUS, LSIL, HSIL and SCC, the detection rates of HPV infection were 60.3 %, 71.8%, 80.3%,100 % respectively. The positive predict value (PPV) of HPV genotype detection was 74.1% and negative predict value(NPV) was 68.7%. The PPV and NPV of TCT were 63.2% and 72.5%, and HPV joint TCT were 75.5% and 100%. The PPV of group H and group H+T was higher than that of group T. The NPV of group H+T were higher than that of group H and group T.Conclusions:The genotyping technology for detection HPV has very high accuracy, joint TCT screen diseased cells effectively in cervical lesions, and can provide more reliable scientific basis for the prevention and treatment of cervical cancer and for the use of HPV vaccine in Qingdao.%目的:探讨人乳头瘤病毒(HPV)分型检测和液基细胞学(TCT)检查在筛查宫颈病变中的应用。方法:回顾性分析2012.9~2013.9在我院妇科门诊就诊,同时采用HPV分型和TCT检查的门诊妇女宫颈脱落细胞标本1128例,并最终进行组织病理学检查。结果:1128例标本中HPV阳性率为64.4%(726/1128),共检出21种亚型,高危亚型16种,低危亚型5种,高危型以16,58,52多见,构成比分别为25.0%,12.1%和10.2%。低危型以61,11型

  1. Performance of the Aptima high-risk human papillomavirus mRNA assay in a referral population in comparison with Hybrid Capture 2 and cytology.

    Science.gov (United States)

    Clad, Andreas; Reuschenbach, Miriam; Weinschenk, Johanna; Grote, Ruth; Rahmsdorf, Janina; Freudenberg, Nikolaus

    2011-03-01

    This study compared the Aptima human papillomavirus (HPV) (AHPV; Gen-Probe Incorporated) assay, which detects E6/E7 mRNA from 14 high-risk types, the Hybrid Capture 2 HPV DNA (HC2; Qiagen Incorporated) test, and repeat cytology for their ability to detect high-grade cervical lesions (cervical intraepithelial neoplasia grade 2+ [CIN2+]) in women referred to colposcopy due to an abnormal Papanicolaou (Pap) smear. A total of 424 clinical specimens, stored in liquid-based cytology (LBC) vials at room temperature for up to 3 years, were tested by repeat cytology, the AHPV assay, and the HC2 test. Assay results were compared to each other and to histology results. The overall agreement between the AHPV assay and the HC2 test was 88.4%. The sensitivity (specificity) of cytology, the HC2 test, and the AHPV assay for the detection of CIN2+ was 84.9% (66.3%), 91.3% (61.0%), and 91.7% (75.0%) and for the detection of CIN3+ was 93.9% (54.4%), 95.7% (46.0%), and 98.2% (56.3%), respectively. Of the disease-positive specimens containing high-risk HPV (HR HPV) DNA as determined by Linear Array (Roche Diagnostics), the AHPV assay missed 3 CIN2 and 1 microfocal CIN3 specimen, while the HC2 test missed 6 CIN2, 4 CIN3, and 1 cervical carcinoma specimen. The AHPV assay had a sensitivity similar to but a specificity significantly higher (P < 0.0001) than the HC2 test for the detection of CIN2+. The AHPV assay was significantly more sensitive (P = 0.0041) and significantly more specific (P = 0.0163) than cytology for the detection of disease (CIN2+).

  2. Liquid-based cytologic screening on cervical lesion:a study of 38 088 cases%宫颈液基细胞学检查对宫颈病变的筛查价值-附38088例分析

    Institute of Scientific and Technical Information of China (English)

    贾英; 吴瑾; 唐良萏; 廖光东; 张新华

    2011-01-01

    目的:探讨液基细胞学检查(Thinprep cytology test,TCT)对宫颈病变的筛查价值.方法:回顾性分析2003年7月至2007年7月在重庆医科大学附属第一医院妇科38 088例TCT结果,阳性者定期复查或行阴道镜检查,必要时在阴道镜引导下定位活检.对有完整资料的1 556例病理结果按年龄进行分组分析,并对TCT与活检组织病理结果的相关性进行统计学分析.结果:38 088例TCT检测中,异常者2 462例,有明确阴道镜活检病理结果者1 556例,病理证实CINⅠ、CINⅡ、CINⅢ、宫颈癌及HPV感染病例分别为279、314、365、75例和94例.TCT与病理结果符合率为60.35%,TCT筛查宫颈病变的灵敏度为84%,特异度为53%,阳性预测值为83%,阴性预测值为56%.30~39岁为宫颈上皮内瘤变和人乳头瘤病毒的高发年龄段、宫颈癌的高发年龄段为30~49岁.TCT阳性者发生CINⅠ、CINⅡ和CINⅢ的相对危险度分别为:非典型鳞状细胞为11.11、13.21、8.19,鳞状上皮内低度病变为22.60、20.72、13.66,鳞状上皮内高度病变为10.33、25.45、54.65.结论:TCT筛查宫颈病变的阳性率、阳性预测值与活检病理结果符合率均较高,对病理结果有一定的预测价值,TCT阳性者宫颈病变的危险较一般人群明显升高.%To investigate the accuracy of Thin-Prep-liquid-based cytology test for cervical lesion with histopathological diagnosis as the criteria. Methods:TCT results of 38088 cases admitted to the First Affiliated Hospital of Chongqing Medical U- niversity from July 2003 to July 2007 were retrospectively studied. The patients with positive result underwent regular check or col- poscopy,and,if necessary,cervical biopsy under guidance of colposcope. A total of 1 556 samples with complete data were divided into groups and analyzed. The relativity of TCT results to biopsy pathological diagnosis was statistically analyzed. Results: The results of TCT examination for 38 088 cases indicated that 2 462

  3. Abnormal Pap Smear and Diagnosis of High-Grade Vaginal Intraepithelial Neoplasia: A Retrospective Cohort Study.

    Science.gov (United States)

    Sopracordevole, Francesco; Mancioli, Francesca; Clemente, Nicolò; De Piero, Giovanni; Buttignol, Monica; Giorda, Giorgio; Ciavattini, Andrea

    2015-10-01

    The aim of this study was to analyze the correlation between the first diagnosis of high-grade Vaginal Intraepithelial Neoplasia (HG-VaIN: VaIN 2-VaIN 3) and the cytological abnormalities on the referral pap smear.All the women with histological diagnosis of HG-VaIN consecutively referred to the Gynecological Oncology Unit of the Aviano National Cancer Institute (Aviano, Italy) from January 1991 to April 2014 and with a pap smear performed in the 3 months before the diagnosis were considered, and an observational cohort study was performed.A total of 87 women with diagnosis of HG-VaIN were identified. Major cytological abnormalities (HSIL and ASC-H) on the referral pap smear were significantly more frequent than lesser abnormalities (ASC-US and LSIL) in postmenopausal women (64.9% vs 36.7%, P = 0.02) and in women with a previous diagnosis of HPV-related cervical preinvasive or invasive lesions (70.5% vs 39.5%, P = 0.01). Diagnosis of VaIN 3 was preceded by major cytological abnormalities in most of the cases (72.7% vs 27.3%, P diagnosis of HG-VaIN can be preceded by different abnormalities on referral pap smear. Major abnormalities are usually reported in postmenopausal women and in women with previous cervical HPV-related disease. However, ASC-US or LSIL do not exclude HG-VaIN, especially VaIN2. An accurate examination of the whole vaginal walls (or vaginal vault) must be performed in all the women who underwent colposcopy for an abnormal pap smear, and a biopsy of all suspicious areas is mandatory. PMID:26496321

  4. Implementation and evaluation of a National External Quality Control Program for Cervical Cytology in Mexico Instrumentación y evaluación de un programa nacional de control de calidad externo para citología cervical en México

    Directory of Open Access Journals (Sweden)

    Ana Flisser

    2002-09-01

    Full Text Available Objective. To evaluate cytology laboratories and the performance of cytotechnologists for establishing efficient external quality control for Mexico's National Program for the Prevention and Control of Cervical Cancer. Material and Methods. During January and February 1998, an on-site evaluation of all cytology laboratories of the Ministry of Health found that only 70% of the microscopes were in adequate working conditions, reagents were out of date, and working conditions were sub-optimal. A program for external quality control based on proficiency testing was established for cytotechnologists. Fifty slide sets with 20 Papanicolaou slides and 10 photographic slides were prepared. The sets were given to the cytotechnologists for evaluation and again one year later by courier. Results. Twenty-one percent of microscopes were repaired and 9% replaced; reagents were distributed and laboratory facilities improved. Only 16% of cytotechnologists passed the initial proficiency test. Cytotechnologists received a refresher training course: one year later 67% of them passed the proficiency test. To ascertain that each slide was correctly diagnosed, 41 sets were rescreened by expert cytopathologists or cytologists and their diagnoses compared to the original ones. Thirty-seven sets had 86% to 96% concordance. Conclusions. This new system for external quality control of cervical cytology allowed the opportune and reliable evaluation of the performance of cytotechnologists.Objetivo. Evaluar los laboratorios de citología y la capacidad de los citotecnólogos para establecer un programa eficiente de control de calidad externo para el Programa Nacional de Prevención y Control de Cáncer Cérvico Uterino de México. Material y métodos. Se realizó una evaluación de todos los laboratorios de citología de la Secretaría de Salud; sólo 70% de los microscopios estaban en buenas condiciones de trabajo, había reactivos caducos y condiciones subóptimas de

  5. 阴道脱落细胞检查联合人类染色体末端酶基因、c-myc 检测在宫颈癌诊断中的价值%The clinical value of Thinprep cytology test combined with h -TERC and c -myc in the diagnosis of cervical ;cancer

    Institute of Scientific and Technical Information of China (English)

    毛海波

    2015-01-01

    Objective To explore the clinical value of Thinprep cytology test (TCT)combined with h -TERC and c -myc in the diagnosis of cervical cancer.Methods hTERC amplification was detected by dual -color interphase fluorescence in situ hybridization (FISH),and the results were compared with TCT and histological examination.Examination the positive which TCT,h -TERC and c -myc by pathological examination.The final diag-nosis was determined by the pathological examination.Results TCT was abnormal in 26.4% of 500 case,18.0%abnormal h -TERC gene,16.0% abnormal c -myc gene.In 270 cases according to the cervical biopsy,the positive rate of chronic inflammation,cervical intraepithelial neoplasia (CIN)Ⅰ,CINⅡ,CINⅢ and cervical cancer:44.4%, 38.2%,36.4%,18.2%,and 7.3% respectively.The positive rates of h -TERC were 18.1%,45.4%,52.5%, 65.9% and 100.0%,respectively.The positive rates of c -myc were 21.4%,48.9%,56.7%,59.9% and 100.0%.With increased pathological grade,the expressions of h -TERC and c -myc were high.Conclusion TCT combined with h -TERC and c -myc can test cervical cancer more effective.%目的:探讨阴道脱落细胞检查(TCT)结合人类染色体末端酶基因(h-TERC)和 c-myc 检测在宫颈癌中的价值。方法运用免疫荧光原位杂交技术检测近三年来该院宫颈癌患者500例的宫颈脱落细胞中h-TERC 和 c-myc 的表达,将其检测结果与 TCT 检测结果比较,将上述三种结果的任一阳性检测再进行病理学诊断标准来确定,且以病理诊断为准进行分析。结果在所检测的500例患者中,TCT 异常者132例(26.4%),h-TECR 异常者90例(18.0%),c-myc 异常者80例(16.0%),将270例患者进行阴道宫颈活检技术,在这些病例中,宫颈慢性炎者120例,宫颈病变者150例,其中宫颈上皮瘤变 CINⅠ52例(38.2%),CINⅡ50例(36.4%),CIN Ⅲ30例(18.2%),宫颈癌18例(7.3%)。在所检测的病例中,

  6. Clinical Significance of HC2-HPV Assay Combined with Liquid Based Cytology in the Screen of Cervical Cancer and Precancerous Lesions%HC2-HPV 检测法联合液基细胞学在宫颈癌及癌前病变筛查中的临床意义

    Institute of Scientific and Technical Information of China (English)

    徐艺; 杨静秀; 王玉平

    2016-01-01

    目的:探讨HC2-HPV检测法联合液基细胞学在筛查宫颈癌及癌前病变中的意义。方法应用HC2-HPV检测法联合液基细胞学技术对体检的患者进行免费宫颈癌及癌前病变的筛查。对不典型增生患者或(和) HC2-HPV检测阳性的患者行阴道镜下宫颈活检。结果液基细胞学检查对宫颈高度病变的检出率为11.0%(11/100);HC2-HPV检测对宫颈高度病变的检出率为27.2%(22/81),对宫颈癌的检出率高达100.0%。 HC2-HPV检测法联合液基细胞学检测阳性与病理检查阳性符合率为64.2%(52/81),显著优于TCT(52.0%)单独检查时的阳性符合率(P<0.05,χ2=11.39)。结论 HC2-HPV检测法联合液基细胞学检测对宫颈癌及癌前病变的筛查具有重要的临床推广价值。%Objective To investigate the significance of HC 2-HPV assay combined with liquid based cytology in the screen of cervical cancer and precancerous lesions .Methods HC2-HPV assay combined with liquid based cytology was used in the screen of cervical cancer and precancerous lesions .Cervical biopsy was performed in patients with atypical hyperplasia or (and) HC2-HPV positive patients.Results Detection rate of liquid based cytology for cervical lesion was 11.0%(11/100),and detection rates of HC2-HPV for cervical lesion and cervical cancer were 27.2%(22/81) and 100.0%.The coincidence rate of HC2-HPV combined with liquid based cytology and biopsy results was 64.2%(52/81),which was significantly higher than that of TCT(52.0%)(P<0.05,χ2 =11.39).Conclusion HC2-HPV assay combined with liquid based cytology test has important clinical value in the screen of cervical cancer and precancerous lesions .

  7. Randomized double-blind trial of beta-carotene and vitamin C in women with minor cervical abnormalities

    OpenAIRE

    Mackerras, D; Irwig, L.; Simpson, J M; Weisberg, E; Cardona, M.; Webster, F.; Walton, L.; Ghersi, D

    1999-01-01

    A double-blind, placebo-controlled, randomized, factorial study using a daily oral administration of 30 mg beta-carotene and/or 500 mg vitamin C was conducted in 141 women with colposcopically and histologically confirmed minor squamous atypia or cervical intra-epithelial neoplasia (CIN) I. Over approximately 2 years of follow-up, 43 lesions regressed to normal and 13 progressed to CIN II. The regression rate was slightly higher, but not significantly so, in those randomized to beta-carotene ...

  8. CLINICAL ASSESSMENT AND CORRILATION OF PAP SMEAR AND LIQUID BASED CYTOLOGY IN BAD CERVIX

    Directory of Open Access Journals (Sweden)

    Khushboo

    2014-10-01

    Full Text Available AIM: Aim of our study to analyze the prevalence of premalignant lesion based on Pap smear and liquid based cytology in patients of bad cervix. OBJECTIVE: To compare sensitivity and specificity of two methods for screening of bad cervix and to know IDR (increase detection rate of cervical cancer by conventional Pap smear and liquid based cytology. MATERIAL AND METHOD: 200 women attending Gynaecology OPD were random selection on the basis of inclusion criteria. All 200 selected women were subjected for down staging through per speculum examination for identification of bad cervix. Pap smears of all 200 selected women were taken and ensured that no local douche, antiseptic cream and no local internal examination was done on the day of test. The prepared smears were then stained according to Papanicolaou's technique. Liquid based cytology smears preparing by using cervical brush 1-1.5cm were inserted into the cervical os until the large outer bristles of the brush touch ectocervix. Data collected for sociodemographic, parity, down staging clinical examination Pap smear and LBC was organised, interpreted and analysis on appropriate statistical software. P value < 0.05 is considered significant RESULT: Analysis revealed that the maximum number of women in our study are from middle age group(31-40yrs, low socioeconomic status, married before 18 yrs, multipara, do not use any contraceptive, uneducated, urban, Muslim population.in this study more abnormal smear is seen in LBC as compared to pap smear. Sensitivity and specificity for LBC is more as compare to pap smear. CONCLUSION: In low resource setting like ours were facilities for radiology, chemo- radiotherapy and supportive care are limited or unavailable. It is important to identify which resources fill healthcare need most effectively and to consider alternative approaches, LBC is strongly advocated in the best interest of public health, by improving the quality of the sample and reducing the

  9. Separation of Normal and Premalignant Cervical Epithelial Cells Using Confocal Light Absorption and Scattering Spectroscopic Microscopy Ex Vivo

    Directory of Open Access Journals (Sweden)

    Ling Yang

    2011-01-01

    Full Text Available Confocal light absorption and scattering spectroscopic (CLASS microscopy can detect changes in biochemicals and the morphology of cells. It is therefore used to detect high-grade cervical squamous intraepithelial lesion (HSIL cells in the diagnosis of premalignant cervical lesions. Forty cervical samples from women with abnormal Pap smear test results were collected, and twenty cases were diagnosed as HSIL; the rest were normal or low-grade cervical squamous intraepithelial lesion (LSIL. The enlarged and condensed nuclei of HSIL cells as viewed under CLASS microscopy were much brighter and bigger than those of non-HSIL cells. Cytological elastic scattered light data was then collected at wavelengths between 400 and 1000 nm. Between 600 nm to 800 nm, the relative elastic scattered light intensity of HSIL cells was higher than that of the non-HSIL. Relative intensity peaks occurred at 700 nm and 800 nm. CLASS sensitivity and specificity results for HSIL and non-HSIL compared to cytology diagnoses were 80% and 90%, respectively. This study demonstrated that CLASS microscopy could effectively detect cervical precancerous lesions. Further study will verify this conclusion before the method is used in clinic for early detection of cervical cancer.

  10. Clinical value of high-risk human papillomavirus DNA test,liquid-based cytology test in diagnosis of cervical cancer and precancerous lesion%高危型人乳头状瘤病毒DNA、液基薄层细胞检测在宫颈癌及前期病变诊断中的临床价值

    Institute of Scientific and Technical Information of China (English)

    杨秀珍

    2014-01-01

    Objective To investigate the clinical significance of high-risk human papillomavirus (HPV) DNA test com-bined with cytology inspection in the screening of cervical precancerous lesions. Methods 5080 women were selected from February 2013 to January 2014 in our hospital,they were given screen of cervical precancerous lesions.Cervical cytology,high-risk HPV DNA testing,colposcopy and cervical biopsy were done in them,and the results were analyzed with statistical methods. Results ASC-US (atypical squamous meaning is not clear epithelial cells) group of 318 cases, LSIL (low-grade squamous intraepithelial lesions) 26 cases of group,HSIL (high-grade squamous intraepithelial lesion) group of 52 cases,6 cases of invasive group confirmed by histopathological examination. Sensitivity and negative predic-tive value of HPV +TCT (liquid-based cytology test) to detect cervical lesions were higher than those high-risk HPV DNA and TCT,the specificity of TCT detection of cervical lesions was higher than high-risk HPV DNA and HPV DNA+ TCT,the difference was significant (P<0.05). Conclusion The sensitivity and negative predictive value of high-risk HPV DNA testing combined with cytology testing in the cervical cancer screening are higher,and has positive im-plications for screening for cervical lesions.%目的:探讨高危型人乳头状瘤病毒(HPV)DNA检测联合宫颈细胞学检查对宫颈癌前病变筛查的临床意义。方法5080名女性均在2013年2月~2014年1月选择在本院就诊并进行宫颈癌前病变筛查,对其进行宫颈细胞学检查、高危型HPV DNA检测、阴道镜下行宫颈活检,使用统计学方法分析检测结果。结果经组织病理检查确诊ASC-US(不能明确意义的非典型鳞状上皮细胞)组318例,LSIL(低度鳞状上皮内病变)组26例,HSIL(高度鳞状上皮内病变)组52例,浸润癌组6例,HPV DNA+液基薄层细胞检测(TCT)检测宫颈病变的灵敏度及阴性预测值高于高危型HPV DNA检测及TCT,TCT

  11. An audit of cervicovaginal cytology in a teaching hospital: Are atypical glandular cells under-recognised on cytological screening?

    Directory of Open Access Journals (Sweden)

    Crasta Julian

    2009-01-01

    Full Text Available Background: Cervical cytology screening for carcinoma of the cervix in India is mainly opportunistic in nature and is practiced mainly in urban centres. The effectiveness of cervical cytology screening depends on various factors. The quality of cervicovaginal cytology service is assessed by various quality indices and by cyto-histology correlation, which is the most important quality assurance measure. Aims: To describe the cervical cytology diagnoses, estimate the quality indices, and evaluate the discrepant cases on cytohistological correlation. Settings and Design: Retrospective observational study from a tertiary care centre in South India. Materials and Methods: Using a database search, all the cervicovaginal cytology reported during the period of 2002-2006 was retrieved and various diagnoses were described. The data was analysed to assess the quality indices. The cytohistologically discrepant cases were reviewed. Results: A total of 10,787 cases were retrieved, of which 98.14% were labeled negative and 1.36% were unsatisfactory for evaluation. A few (0.81% of the cases were labeled as squamous intraepithelial lesions and 0.38% as atypical squamous cells. The ASCUS: SIL ratio was 0.5. Cytohistological correlation revealed a total of ten cases with significant discrepancy. The majority of these were carcinomas that were misdiagnosed as atypical glandular cells. These cytology smears and the subsequent biopsies were reviewed to elucidate the reasons for the discrepancies. Conclusions: The cervical cytology service at our centre is well within the accepted standards. An increased awareness of cytological features, especially of glandular lesions, a good clinician-laboratory communication and a regular cytohistological review would further improve the diagnostic standards.

  12. 249例细胞学、HPV-DNA分型检测在宫颈癌初筛中的意义分析%Analysis on significances of cytology and HPV-DNA genotyping in primary screening of 249 cases with cervical cancer

    Institute of Scientific and Technical Information of China (English)

    林莉; 林清平; 陈小苑; 徐朝斌

    2013-01-01

    Objective:To analyze and understand the significances of cytology and HPV-DNA genotyping in primary screening of cervical cancer by conducting cytology test,HPV-DNA genotyping,and biopsy among patients from the hospital from August 10th,2010 to October 1 st,2011.Methods:Cytological screening:all the patients were detected by liquid-based Thin Prep cytology test (TCT) ; the diagnostic criterion of TCT was TBS classification; HPV-DNA genotyping:cervical canal samples of all the patients were collected by sampling kit of dot blot,supervising technicians conducted dot blot and analyzed the results,21 kinds of cancer type HPV DNA currently known were detected,including 13 high risk HPV types and 8 low risk and common HPV subtype.All the women underwent electronic colposcopy (SLC-2000,Shenzhen),the operation was conducted by specialized obstetricians and gynecologists,and suspicious lesions underwent colposcopic localization and biopsy.Results:The total sensitivity of joint detection of cytological test and HPV-DNA genotyping was 91.82%,which was significantly higher than those of simple cytological test and simple HPV-DNA genotyping,especially for cervical intraepithelial neoplasia Ⅱ or above lesions,the sensitivity was as high as 100.00%,so joint detection of cytological test and HPV-DNA genotyping was the most reliable method for screening of cervical cancer,which could reduce the missed diagnostic rate to a minimal level.Conclusion:Cytological test is the most reliable method for primary screening of cervical cancer currently,HPV-DNA genotyping only can assist cytological test for primary screening,but it cant replace cytological test completely.HPV-DNA genotyping is a best method for treatment of ASCUS,which can isolate high risk population of ASCUS effectively.Compared with simple cytological test,the sensitivity of joint detection of cytological test and HPV-DNA genotyping increases,but the screening cost increases greatly,it is recommended to use the method

  13. Avaliação da frequência de realização do exame físico das mamas, da colpocitologia cervical e da ultrassonografia obstétrica durante a assistência pré-natal: uma inversão de valores Evaluation of the frequency of accomplishment of the breast examination and of oncotic cervical cytology and the obstetrical ultrasound during the prenatal period: an inversion of values

    Directory of Open Access Journals (Sweden)

    Carla Vitola Gonçalves

    2009-01-01

    Full Text Available INTRODUÇÃO: Embora o exame de ultrassonografia seja um procedimento frequente na gravidez, o seu uso rotineiro não demonstrou efetividade sobre a redução da morbi-mortalidade materna ou perinatal. OBJETIVOS: Avaliar a cobertura do exame das mamas e da citologia oncótica cervical entre os exames de pré-natal, comparando-se com o número de ultrassonografias obstétricas realizadas. MÉTODOS: Constituiu-se de uma avaliação transversal, realizada na cidade de Rio Grande (RS, entre maio e julho de 2007. Os dados obtidos por meio de um questionário estruturado foram digitados no programa Epi-Info 6.04 e analisados no software SPSS. RESULTADOS: Entre as 230 puérperas entrevistadas, 99,1% realizaram ultrassonografia durante a gravidez, enquanto 37,5% e 33,6% realizaram exame clínico das mamas ou colpocitologia cervical, respectivamente. Houve significância estatística (p-valor Although ultrasound examination is a frequent procedure during pregnancy, routine use has not been effective to reduce maternal or perinatal morbidity-mortality. OBJECTIVE: Assess frequency of breast examination and of oncotic cervical cytology among prenatal exams and compare it to frequency of obstetrical ultrasound. METHODS: This was a cross-sectional evaluation conducted in the city of Rio Grande, RS, Brazil between May and July 2007. Data obtained with a structured questionnaire were entered into the Epi-Info 6.04 program and analyzed using the SPSS software. RESULTS: Among the 230 puerperae interviewed, 99.1% had undergone an ultrasound during pregnancy, while 37.5% and 33.6% had been submitted to clinical examination of the breasts and cervical cytology, respectively. It was noted that a larger number of obstetrical echographies increased the probability that a patient would be submitted to clinical examination of the breasts. On the other hand, the number of cytopathological evaluations of the uterine cervix was not influenced by the number of ultrasound

  14. 宫颈液基薄层细胞学(LCT)及TBS报告系统在宫颈病变诊断中的应用价值%Study application value of Liquid-based thinlayer cytology and TBS for cervical diseases diagnosis

    Institute of Scientific and Technical Information of China (English)

    汤峻; 范自然

    2010-01-01

    目的 探讨宫颈液基薄层细胞制片技术(LCT)及TBS报告系统在宫颈病变检查中的应用价值.方法 收集我院1257例宫颈细胞标本,采用液基薄层细胞制片技术和TBS报告系统诊断,对细胞学诊断阳性的标本进行阴道镜活检和组织病理学诊断,所有病例的诊断均双盲进行.结果 1257例中细胞学检出鳞状细胞癌(SCC)8例,高级别鳞状上皮病变(HSIL)21例,低级别鳞状上皮病变(LSIL)34例,与活检病理诊断符合例数分别为:SCC 100.00%(8/8),HSIL 90.47%(19/21)和LSIL 94.11%(32/34).结论 宫颈液基薄层细胞制片技术和TBS报告系统对宫颈病变检出阳性率较高,与组织学诊断符合率高,是临床宫颈病变检查的好方法.%Objective To study the value of liquid-based thinlayer cytology and TBS for cervical diseases diagnosis. Methods This study screen 1257women from August 2007 to August 2009 in this hospital. The cytologic diagnosis was performed using the Bethesda system (TBS)on the liquid-based thinlayer cytology. Theresults of cytologic diagnosis compare with the results of histopathologic diagnosis. All the diagnosises were performed in an independent and blinded fashion. Results All cases were able to detect 100% ( 8/8 ) of squamous cell carcinoma ( SCC )1, 90.47% ( 19/21 ) high grade squamous intraepithelial lesion ( HSIL), 94.11% ( 23/34 )low grade squamous intraepithelial lesion ( LSIL). Conclusion The liguidbased thinlayer cytology test and the Bethesda System(TBS) is a sensitive of cervical cancer diagnosis.

  15. A lectin-based diagnostic system using circulating antibodies to detect cervical intraepithelial neoplasia and cervical cancer.

    Science.gov (United States)

    Jin, Yingji; Kim, Seung Cheol; Kim, Hyoung Jin; Ju, Woong; Kim, Yun Hwan; Kim, Hong-Jin

    2016-01-01

    In the present study, we developed serological strategies using immunoglobulin fractions obtained by protein A chromatography to screen for cervical cancer and cervical intraepithelial neoplasia I (CIN I). The reactivities of the immunoglobulins purified from sera of women with normal cytology, CIN I and cervical cancer were compared in enzyme-linked immunosorbent assays (ELISA) and enzyme-linked lectin assays (ELLAs). To capture the immunoglobulins, ELISAs and ELLAs were performed in protein A immobilized microplates. The reactivity of immunoglobulin in ELISA was in the increasing order normal cytology, CIN I and cervical cancer, while that in ELLAs for detecting fucosylation was in the decreasing order normal cytology, CIN I and cervical cancer. It was confirmed that women with CIN I were distinguishable from women with normal cytology or women with cervical cancer in the ELISA or the ELLA for detecting fucosylation with considerable sensitivity and specificity. Women with cervical cancer were also distinguishable from women with normal cytology with high sensitivity (ELISA: 97%, ELLA: 87%) and specificity (ELISA: 69%, ELLA: 72%). Moreover, the logistic regression model of the ELISA and the ELLA discriminated cervical cancer from normal cytology with 93% sensitivity and 93% specificity. These results indicate that the ELISAs and the ELLAs have great potential as strategies for primary screening of cervical cancer and CIN. It is expected that the ELISA and the ELLA can provide new insights to understand systemic changes of serum immunoglobulins during cervical cancer progression.

  16. Optoelectronic method for detection of cervical intraepithelial neoplasia and cervical cancer

    Science.gov (United States)

    Pruski, D.; Przybylski, M.; Kędzia, W.; Kędzia, H.; Jagielska-Pruska, J.; Spaczyński, M.

    2011-12-01

    The optoelectronic method is one of the most promising concepts of biophysical program of the diagnostics of CIN and cervical cancer. Objectives of the work are evaluation of sensitivity and specificity of the optoelectronic method in the detection of CIN and cervical cancer. The paper shows correlation between the pNOR number and sensitivity/specificity of the optoelectronic method. The study included 293 patients with abnormal cervical cytology result and the following examinations: examination with the use of the optoelectronic method — Truscreen, colposcopic examination, and histopathologic biopsy. Specificity of the optoelectronic method for LGSIL was estimated at 65.70%, for HGSIL and squamous cell carcinoma of cervix amounted to 90.38%. Specificity of the optoelectronic method used to confirm lack of cervical pathology was estimated at 78.89%. The field under the ROC curve for the optoelectronic method was estimated at 0.88 (95% CI, 0.84-0.92) which shows high diagnostic value of the test in the detection of HGSIL and squamous cell carcinoma. The optoelectronic method is characterised by high usefulness in the detection of CIN, present in the squamous epithelium and squamous cell carcinoma of cervix.

  17. Liquid-Based Cytology of Villoglandular Adenocarcinoma of the Cervix: A Report of 3 Cases

    OpenAIRE

    Choi, Younghwa; Kim, Haeryoung; Choi, Haiyoung; Hwang, Daehyun; Choe, Gheeyoung; Chung, Jin-Haeng; Park, So Yeon; Lee, Hye Seung; Paik, Jin Ho; Park, Hyo Jin

    2012-01-01

    Villoglandular adenocarcinoma (VGA) is a rare subtype of cervical adenocarcinoma with a more favorable prognosis compared to conventional adenocarcinomas. Although the tumors are usually recognized on colposcopic examination due to the mainly exophytic growth pattern, they may be underdiagnosed as benign lesions by cytology because of their minimal cytologic atypia. We report the liquid-based cytology (LBC) findings of three histologically confirmed VGAs which we have recently identified. The...

  18. High grade squamous intraepithelial lesion in inmates from Ohio: cervical screening and biopsy follow-up

    Directory of Open Access Journals (Sweden)

    Rofagha Soraya

    2006-01-01

    Full Text Available Abstract Background Cervical carcinoma remains the second leading cause of cancer death in women worldwide and sexual behavior is regarded as the main contributing factor. We studied cervical cytology screening with surgical biopsy follow-up in women prisoners and compared the findings to those in the general population. Methods We reviewed 1024 conventional cervical smears, 73 cervical biopsies and 2 loop electrosurgical excision procedure (LEEP specimens referred to us from the Correctional Center in Columbus, Ohio during a 12-month period. The results were compared to 40,993 Pap smears from the general population for the same 12-month period. Results High grade squamous intraepithelial lesion (HGSIL was diagnosed in 1.3% of the cervical smears from the inmate population versus 0.6% in the general population (p < 0.01. The unsatisfactory rate was 1.6% compared to 0.3% in the general population (p < 0.01. Among the study population, follow-up tissue diagnosis was obtained in 24.3% of the abnormal cytology results (ASCUS, LGSIL, and HGSIL. Of the HGSIL Pap smears, 61.5% had a subsequent tissue diagnosis. Thirty-nine biopsies (52% of the all inmate biopsies and LEEP showed CIN II/III (cervical intraepithelial neoplasia II/III. Eight of these thirty-nine follow-up biopsies diagnosed as CIN II/III had a previous cervical cytology diagnosis of ASCUS. The average age for HGSIL was 30.5 years (S.D. = 5.7 and for low grade squamous intraepithelial lesion (LGSIL was 27.2 years (S.D. = 6.1. Conclusion A significantly higher prevalence of HGSIL cervical cytology and unsatisfactory smears was encountered in female inmates, with tissue follow-up performed in less than two thirds of the patients with HGSIL. These results are in keeping with data available in the literature suggesting that the inmate population is high-risk and may be subject to less screening and tissue follow-up than the general population. Clinicians should proceed with urgency to improve

  19. 陕西地区宫颈癌和细胞学正常妇女 HR-HPV病毒流行谱分析%High risk human papillomavirus prevalence in women with normal cytology and with cervical cancer in Shaanxi area

    Institute of Scientific and Technical Information of China (English)

    杨波; 曹萌; 齐静娴; 李娇; 朱广侠; 张冠军; 王一理

    2015-01-01

    Objective To analyze the spectrum of HPV in women with normal cytology and cervical cancer with high risk human papillomavirus (HR-HPV) in Shaanxi area.Methods HPV16, 18, 31, 33, 35, 45, 52, 58 DNA in cervical exfoliated cells were detected by PCR.Pap tests were performed for cytopathological analysis.Infection spectrum was analyzed and compared between 1 005 cases with positive HR-HPV and normal cytology and 160 cases of cervical cancer.Results The distribution of HPV16, 18, 31, 33, 52 and 58 was significantly different between normal cytology group and cervical cancer group except for HPV35 and 45 (χ2 value was 465.147, 110.288, 9.261, 4.496, 30.155 and 46.559, respectively, all P<0.05).Infection with single HPV type was more common in the normal cytology group, while infection with multiple HPV types were frequently found in the cervical cancer group.HPV16 was the most prevalent genotype detected in two groups, and the infection rate of it was 34.9% and 37.6% respectively in the normal cytology group and cervical cancer group.Considering multiple infection, HPV16 and 52 were more frequently found in the normal cytology group (2.9%), while in the cervical cancer group the most prevalent multiple infection pattern were HPV16 and 18 (20.8%).Conclusion The difference in the distribution of HPV types found both in normal cytology group and cervical cancer group is helpful for understanding HPV carcinogenic mechanism and designing of prophylactic vaccine.%目的:分析陕西地区高危型人乳头瘤病毒( HR-HPV)阳性,但宫颈细胞学正常和宫颈癌患者HPV病毒谱。方法利用PCR基因扩增方法,检测宫颈脱落细胞中8种HR-HPV DNA(包括HPV16、18、31、33、35、45、52、58等)并行细胞病理学检测。对1005例HR-HPV阳性但宫颈细胞学正常标本及160例经组织病理学证实宫颈癌标本进行HPV感染谱对比分析。结果正常细胞学组与宫颈癌组的HR-HPV型别分布除HPV35、45

  20. Epidemiology and Early Detection of Cervical Cancer.

    Science.gov (United States)

    Hillemanns, Peter; Soergel, Phillip; Hertel, Hermann; Jentschke, Matthias

    2016-01-01

    The new German S3 guideline 'Prevention of Cervical Cancer' published in 2016 is based on the latest available evidence about cervical cancer screening and treatment of cervical precancer. Large randomized controlled trials indicate that human papillomavirus (HPV)-based screening may provide better protection against cervical cancer than cytology alone through improved detection of premalignant disease in the first screening round prior to progression. Therefore, women aged 30 years and older should preferably be screened with HPV testing every 3-5 years (cytology alone every 2 years is an acceptable alternative). Co-testing is not recommended. Screening should start at 25 years using cytology alone every 2 years. The preferred triage test after a positive HPV screening test is cytology. Women positive for HPV 16 and HPV 18 should receive immediate colposcopy. Another alternative triage method is p16/Ki-67 dual stain cytology. The mean yearly participation rate in Germany is between 45 and 50%. Offering devices for HPV self-sampling has the potential to increase participation rates in those women who are at higher risk of developing cervical cancer. Regarding primary prevention, the 9-valent vaccine may provide protection against up to 85% of cervical intraepithelial neoplasia (CIN) 3 and 90% of cervical cancer, and is available in Europe as a 2-dose schedule from May 2016. PMID:27614953

  1. Diagnosis of cervical cancer with transvaginal color Doppler sonography

    OpenAIRE

    Li-bo DENG; Wei ZHOU; Chang, Shu-Fang; Ming-jie LIN

    2011-01-01

    Objective To investigate the imaging features of cervical cancer by transvaginal color Doppler sonography(TVCS),and evaluate the diagnostic value of TVCS.Methods A hundred and thirty cases of cervical intraepithelial neoplasia(CIN) grade Ⅰ-Ⅱ and cervical cancer,diagnosed by Thinprep cytologic test(TCT),cervical biopsy and pathological examination,received TVCS examination.The image characters and color Doppler flow imaging(CDFI) were collected and analyzed.Another 41 cases with normal cervice...

  2. Validation of cervical cancer screening methods in HIV positive women from Johannesburg South Africa.

    Directory of Open Access Journals (Sweden)

    Cynthia Firnhaber

    Full Text Available BACKGROUND: HIV-infected women are at increased risk for developing cervical cancer. Women living in resource-limited countries are especially at risk due to poor access to cervical cancer screening and treatment. We evaluated three cervical cancer screening methods to detect cervical intraepithelial neoplasia grade 2 and above (CIN 2+ in HIV-infected women in South Africa; Pap smear, visual inspection with 5% acetic acid (VIA and human papillomavirus detection (HPV. METHODS: HIV-infected women aged 18-65 were recruited in Johannesburg. A cross-sectional study evaluating three screening methods for the detection of the histologically-defined gold standard CIN-2 + was performed. Women were screened for cervical abnormalities with the Digene HC2 assay (HPV, Pap smear and VIA. VIA was performed by clinic nurses, digital photographs taken and then later reviewed by specialist physicians. The sensitivity, specificity and predictive valves for CIN-2 + were calculated using maximum likelihood estimators. RESULTS: 1,202 HIV-infected women participated, with a median age of 38 years and CD4 counts of 394 cells/mm(3. One third of women had a high grade lesion on cytology. VIA and HPV were positive in 45% and 61% of women respectively. Estimated sensitivity/specificity for HPV, Pap smear and VIA for CIN 2+ was 92%/51.4%, 75.8%/83.4% and 65.4/68.5% (nurse reading, respectively. Sensitivities were similar, and specificities appeared significantly lower for the HPV test, cytology and VIA among women with CD4 counts ≤200 cells/mm(3 as compared to CD4 counts >350 cells/mm(3. CONCLUSIONS: Although HPV was the most sensitive screening method for detecting CIN 2+, it was less specific than conventional cytology and VIA with digital imaging review. Screening programs may need to be individualized in context of the resources and capacity in each area.

  3. Clinical Application of Liquid-based Cytology, HPV-DNA or Colposcopy in Cervical Cancer Screening%液基细胞、HPV检测与阴道镜宫颈癌的筛查方面的应用研究

    Institute of Scientific and Technical Information of China (English)

    胡芳艳; 金志红; 刘国华; 杨金艳; 王艳芳

    2013-01-01

    Objective: To compare the diagnosis value of liquid-based cytology, HPV-DNA and colposcopy in early diagnosis of cervical cancer. Methods: 92 cases were retrospectively studied which liquid-based cytology were diagnosed as typical squamous cell of undetermined significance (ASCUS). All these patients were taken human papilloma virus (HPV) DNA detection, colposcopy and biopsies were taken. The results of the different tests were compared with the pathological examination of biopsy for further analysis. Results: The pathological and histological examination of biopsy was set as the standards, and all the results from other test were compared with them. Colposcopy had highest sensitivity in 3 methods (P<0.05), and plays an important role in screening of cervical lesions. The infection rate of HR-HPV increased significantly along with the increase of cervical lesion severity, there were obvious differences between ASCUS and squamous intraepithelial lesion (SIL) (P<0.05). With the grade of biopsy increased, the incidence of positive rate increased in all three methods. The detection rate of CIN and cervical cancer in ASCUS patients were more commonly in the stage of age between 30 and 50. Conclusion: Liquid base cervical cytological examination (LCT) was a good method with higher detection rate for early detection of higher cervical lesions. High-risk type HPV-DNA testing and Colposcopy could reduce the omission diagnose rate and improve the diagnostic accuracy. The cervical lesion screening of women over 30 year-old should cause much attention, especially for the women of 30-50 years old.%目的:探讨液基薄层细胞学检查、HPV-DNA检测、阴道镜检查在宫颈癌早期诊断中的临床应用价值.方法:回顾性分析92例宫颈液基薄层细胞学检查未能明确诊断意义的宫颈非典型鳞状细胞病变患者,进行HPV-DNA检测与阴道镜检查,将检测的结果进行对比研究.结果:阴道镜与宫颈液基薄层细胞检查(TCT)

  4. Cost-effectiveness of conventional cytology and HPV DNA testing for cervical cancer screening in Colombia Costo-efectividad de la citología y la tamización con pruebas de ADN-VPH para cáncer de cuello uterino en Colombia

    Directory of Open Access Journals (Sweden)

    Oscar Andrés-Gamboa

    2008-08-01

    Full Text Available OBJECTIVE: To assess cost-effectiveness of conventional cytology and HPV DNA testing for cervical-cancer screening in Colombia. MATERIAL AND METHODS: The National Cancer Institute of Colombia (NCIC in 2007 developed a Markov model on the natural history of cervical cancer; no screening, conventional cytology, and HPV DNA testing were compared. Only direct costs were used. Outcomes comprise cervical cancer mortality, years of life saved, and lifetime costs. Discounted incremental cost-effectiveness ratios were estimated and sensitivity analyses were conducted for key parameters. RESULTS: Depending on the screening strategy a 69-81% mortality reduction might be expected. The HPV DNA testing every five years is a cost-effective strategy (Incremental Cost-Effectiveness Ratio (ICER: USD$44/YLS if the cost per test is under USD$31. The effectiveness was sensitive to coverage and primarily to follow-up. CONCLUSIONS: HPV DNA testing is a cost-effective alternative for screening in Colombia. Not only high coverage but high follow-up rates are critical for successful screening programs.OBJETIVO: evaluar el costo-efectividad de la citología convencional y la prueba de ADN-VPH para tamización de cáncer cervical en Colombia. MATERIAL Y MÉTODOS: el Instituto Nacional de Cancerología de Colombia construyó en 2007 un modelo de Markov de historia natural del cáncer cervical. Se comparó "no tamización", citología convencional y prueba de ADN-VPH. Se utilizaron costos directos. Los desenlaces fueron mortalidad, años de vida ganados y costos. Se calcularon razones de costo-efectividad incremental. Se realizaron análisis de sensibilidad para parámetros clave. RESULTADOS: la mortalidad se redujo 69-81% según la estrategia. La tamización con ADN-VPH cada cinco años es costo-efectiva (ICER (Razón de Costo-Efectividad incremental por sus siglas en inglés: 44 dólares por año de vida saludable si los costos por prueba son menores a 31 dólares. La

  5. Human Papillomavirus Types 52 and 58 Are Prevalent in Uterine Cervical Squamous Lesions from Japanese Women

    Directory of Open Access Journals (Sweden)

    Kazuhiro Takehara

    2011-01-01

    Full Text Available Objective. To estimate the prevalence and genotypes of high-risk human papillomavirus (HPV focusing HPV 16, 18, 52, and 58 in Japan. Methods. Liquid-base cytology specimens were collected from Japanese women (n=11022, aged 14–98. After classifying cytodiagnosis, specimens were analyzed for HPV DNA by the multiplex polymerase chain reaction method, where 1195 specimens were positive for cervical smear, except adenomatous lesions. Result. HPV genotypes were detected in 9.5% of NILM and 72.2% of ASC-US or more cervical lesions. In positive cervical smears, HPV genotypes were HPV 52 at 26.6%, HPV 16 at 25.2%, HPV 58 at 21.8%, and HPV 18 at 7.1%. Most patients infected with HPV 16 were between 20–29 years old, decreasing with age thereafter. As for HPV 52 and 58, although the detection rate was high in 30- to 39-year-olds, it also was significant in the 50s and 60s age groups. Conclusion. In Japan, as a cause of abnormal cervical cytology, HPV52 and 58 are detected frequently in addition to HPV 16. In older age groups, HPV 52 and 58 detection rates were higher than that observed for HPV 16. After widespread current HPV vaccination, we still must be aware of HPV 52 and 58 infections.

  6. The role of HPV in diagnosis and management of cervical premalignancies

    OpenAIRE

    Hamont, D. van

    2008-01-01

    Cervical cytological pathology is not uncommon. Prevention of cervical cancer by detection of the disease in an early and pre-malignant stage is practised globally either through population-based screening programmes or more optimistically non-organised ones. High-grade cervical intraepithelial neoplasia (CIN) detected by cervical cytological screening are extensively visualised by colposcopy and successively treated by, for instance, large loop electro-surgical excision of the transformation...

  7. Evidence of disrupted high-risk human papillomavirus DNA in morphologically normal cervices of older women.

    Science.gov (United States)

    Leonard, Sarah M; Pereira, Merlin; Roberts, Sally; Cuschieri, Kate; Nuovo, Gerard; Athavale, Ramanand; Young, Lawrence; Ganesan, Raji; Woodman, Ciarán B

    2016-02-15

    High-risk human papillomavirus (HR-HPV) causes nearly 100% of cervical carcinoma. However, it remains unclear whether HPV can establish a latent infection, one which may be responsible for the second peak in incidence of cervical carcinoma seen in older women. Therefore, using Ventana in situ hybridisation (ISH), quantitative PCR assays and biomarkers of productive and transforming viral infection, we set out to provide the first robust estimate of the prevalence and characteristics of HPV genomes in FFPE tissue from the cervices of 99 women undergoing hysterectomy for reasons unrelated to epithelial abnormality. Our ISH assay detected HR-HPV in 42% of our study population. The majority of ISH positive samples also tested HPV16 positive using sensitive PCR based assays and were more likely to have a history of preceding cytological abnormality. Analysis of subsets of this population revealed HR-HPV to be transcriptionally inactive as there was no evidence of a productive or transforming infection. Critically, the E2 gene was always disrupted in those HPV16 positive cases which were assessed. These findings point to a reservoir of transcriptionally silent, disrupted HPV16 DNA in morphologically normal cervices, re-expression of which could explain the increase in incidence of cervical cancer observed in later life.

  8. Squash smear cytology of Langerhans cell histiocytosis

    Science.gov (United States)

    Nahm, Ji Hae; Yoon, Gun; Do, Sung-Im; Kim, Hyun-Soo

    2015-01-01

    Squash smear cytology of Langerhans cell histiocytosis (LCH) has rarely been reported. We described squash cytological findings of cranial LCH. Additionally, based on recent data that suggests an association of LCH with either viral infection or genetic alteration, we investigated the presence of several viruses or mutation of TP53 and BRAF in LCH tissue samples. Intraoperative squash smears of a small tissue fragment excised from the lesion demonstrated a mixed population of eosinophils, neutrophils, small lymphocytes and a high content of histiocytes. The histiocytes possessed abundant dense cytoplasm with round cell shape and eccentrically located nuclei with fine chromatin, delicate nuclear membranes and prominent nuclear grooves, indentations and pseudoinclusions. The cytologic features were consistent with Langerhans cells (LCs). Subsequent histopathologic examination confirmed the diagnosis of LCH. Immunohistochemically, the LCs were positive for S-100, CD1a and langerin, but negative for adenovirus, CMV, EBV, HHV-8, HPV, HSV, SV 40 and p53. BRAF V600E mutation was absent. Our findings did not support the role of viruses and genetic abnormalities in the pathogenesis of LCH. In summary, the presence of a mixed population of inflammatory cells and a high content of histiocytes with characteristic cytomorphology, along with radiologic evidence and appropriate clinical findings, is highly suggestive of LCH on the intraoperative squash smears. Awareness of characteristic cytological features of LCH is necessary for rapid and accurate diagnosis. Squash smear cytology is a potentially useful tool in the intraoperative diagnosis of LCH. PMID:26339366

  9. Role of human papillomavirus testing and cytology in follow-up after conization

    DEFF Research Database (Denmark)

    Gosvig, Camilla F; Huusom, Lene D; Deltour, Isabelle;

    2015-01-01

    OBJECTIVE: Adequate follow-up of women who have undergone conization for high-grade cervical lesions is crucial in cervical cancer screening programs. We evaluated the performance of testing for high-risk human papillomavirus (HPV) types, cytology alone, and combined testing in predicting cervica...

  10. 液基薄层细胞学、活检病理和宫颈环形电切术(LEEP)诊断早期宫颈癌及宫颈上皮细胞内瘤样病变的比较研究%Comparison of Liquid Based Cytology, Biopsy, and Loop Electrosurgical Excisional Procedure(LEEP)for Diagnosis of Cervical Cancer and Cervical Intraepithelial Neoplasia

    Institute of Scientific and Technical Information of China (English)

    杨波; 杨凤云; 曹云桂; 顾萍; 朱梅娟; 甘晓卫

    2012-01-01

    Objective: To compare liquid based cytology, biopsy, and loop electrosurgical excisional procedure (LEEP) histopathology for diagnosis of cervical cancer and cervical intraepithelial neoplasia (CIN). Methods: A total of 201 patients who referred for LEEP were enrolled in this study. The diagnostic results of thinprep cytologic tests (TCT), previous biopsies, and LEEP pathology were compared and evaluated. Results: In LEEP pathology of 201 patients, 92 cases (45.8%) were identified to have CIN2 or above lesion, and 4 cases were confirmed as invasive cancer. Among them, 71 (71/92) biopsy was CIN2 or above cervical lesions. Biopsy and LEEP determined 22 and 23 CIN2 or above cases in 66 LSIL cases by TCT, respectively. The concordance rate of biopsy and LEEP was 86.5%, and the Kappa value was 0.718. Among 113 CIN1 patients conformed by biopsy, LEEP pathology found 16 cases of CIN2 or above. Conclusion: Biopsy may improve the diagnosis of CIN2 or above cervical lesions in LSIL patients by TCT, and will be helpful for determination of index for LEEP in clinical practice.%目的:比较细胞学、活检病理和宫颈环形电切术(loop electrosurgical excisional procedure,LEEP)病理诊断早期宫颈癌和宫颈上皮细胞内瘤样病变(cervical intraepithelial neoplasia,CIN)的准确性.方法:回顾性比较分析201例进行LEEP患者术后病理、术前活检病理和液基薄层细胞学(thinprep cytologic test,TCT)3种诊断方法的检测结果.结果:201例LEEP术后组织病理分析显示92例(45.8%)患者具有CIN2~3病变及4例浸润癌患者,其中71例患者术前病理为CIN2及以上鳞状上皮内瘤样病变.在细胞学检查结果为LSIL的66例患者中活检病理和LEEP病理结果为CIN2及以上病变的分别有22例和23例.活检病理与LEEP病理符合率为86.5%,Kappa值为0.718.活检病理结果为CIN1的113例患者中有16例LEEP病理结果为CIN2及以上病变.结论:活检病理可以提高细胞学结果为LSIL的患

  11. Human papillomavirus status and cervical abnormalities in women from public and private health care in Rio de Janeiro State, Brazil Papilomavírus humano e anormalidades cervicais em mulheres do sistema de saúde privado e público no Estado do Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Ledy H.S. Oliveira

    2006-10-01

    Full Text Available This article reports the HPV status and cervical cytological abnormalities in patients attended at public and private gynecological services from Rio de Janeiro State. It also comments the performance of each HPV DNA tests used. A set of 454 women from private health clinics was tested by routine Capture Hybrid II HPV DNA assay. Among these, 58.4% presented HPV and nearly 90% of them were infected by high risk HPV types. However, this group presented few premalignant cervical lesions and no invasive cervical cancer was registered. We also studied 220 women from low income class attended at public health system. They were HPV tested by polymerase chain reaction using My09/11 primers followed by HPV typing with E6 specific primers. The overall HPV prevalence was 77.3%. They also showed a high percentage of high squamous intraepithelial lesion-HSIL (26.3%, and invasive cervical carcinoma (16.3%. HPV infection was found in 93.1% and 94.4% of them, respectively. The mean ages in both groups were 31.5 and 38 years, respectively. In series 1, HPV prevalence declined with age, data consistent with viral transient infection. In series 2, HPV prevalence did not decline, independent of age interval, supporting not only the idea of viral persistence into this group, but also regional epidemiological variations in the same geographic area. Significant cytological differences were seen between both groups. Normal and benign cases were the most prevalent cytological findings in series 1 while pre-malignant lesions were the most common diagnosis in the series 2. HPV prevalence in normal cases were statistically higher than those from series 1 (p Este artigo analisa a infecção por HPV e anormalidades citológicas cervicais encontradas em pacientes atendidas em serviços ginecológicos dos sistemas de saúde público e privado do estado do Rio de Janeiro. O trabalho também avalia os testes utilizados para detecção de DNA do HPV em cada população estudada

  12. The role of HPV genotyping test and liquid-based cytology in diagnosis of cervical pre-cancerous lesions%人乳头瘤病毒分型基因检测和液基细胞学检查在宫颈癌前病变中的诊断作用

    Institute of Scientific and Technical Information of China (English)

    谢芳; 黎云; 彭秀红; 李洁明

    2011-01-01

    Objective To evaluate the role and significance of HPV genotyping detection and Thinprep cytology test (TCT ) in the diagnosis of cervical precancerous lesions ( mild to moderate epithelial neoplasia ). Methods The findings of HPV genotyping and TCT were analyzed in 401 patients with cervical precancerous lesions ( CIN1-CIN2 ) who had been hospitalized between November 2008 to November 2010. Results The sensitivity of HPV genotyping was significantly higher than that of TCT ( P<0.01 ).TCT combined with HPV genotyping enhanced the detection rate of cervical lesions. Subtypes 16, 52, 58, and 18 of HPV were common in high-risk HPV infection with cervical precancerous lesions. Subtype 16 had a markedly higher rate of occurrence of cervical lesions than subtypes 52 and 58 ( P < 0.01 ). Conclusions High-risk HPV infection is the major pathogenic factor for cervical cancer and precancerous lesions. HPV 16 infection easily leads to cervical lesion. HPV genotyping test combined with Thinprep cytology test can increase the detection rate of cervical precancerous lesions.%目的 评估人乳头瘤病毒(HPV)基因分型检测和液基细胞学检查(TCT)在宫颈轻、中度上皮细胞内瘤变(CIN1-CIN2)中的诊断作用及意义.方法 对我院2008年11月-2010年11月期间401例因宫颈癌前病变(CIN1-CIN2)要求官颈环切的患者的HPV分型基因检测情况与液基细胞学检查结果进行总结分析.结果 HPV的灵敏性较TCT明显增高(P<0.01),TCT和HPV基因分型检测结合能提高宫颈病变的检出率;宫颈癌前病变中HPV感染以高危基因组的16、52、58、18亚型感染多见,HPV16犁感染引起高度病变的几率较HPV52型和HPV58型明显增高(P<0.01).结论 高危HPV感染是宫颈癌及癌前病变发病的主要致病因子,HPV16型感染易导致宫颈高度病变;HPV基因分型检测和液基细胞学检查结合,可以提高宫颈癌前病变的检出率.

  13. Transition of high-grade cervical intraepithelial neoplasia to micro-invasive carcinoma is characterized by integration of HPV 16/18 and numerical chromosome abnormalities.

    NARCIS (Netherlands)

    Hopman, A.H.N.; Smedts, F.; Dignef, W.; Ummelen, M.; Sonke, G.; Mravunac, M.; Vooijs, G.P.; Speel, E.J.; Ramaekers, F.C.S.

    2004-01-01

    Cervical intraepithelial neoplasia (CIN I, II, and III) and cases of CIN III associated with micro-invasive cervical carcinoma (CIN III & mCA) were analysed for evidence of episomal or integrated human papillomavirus (HPV) 16/18 DNA by fluorescence in situ hybridization (FISH). In parallel, numerica

  14. [Preventing cervical cancer].

    Science.gov (United States)

    Simon, P; Noël, J-C

    2015-09-01

    The incidence of cervical cancer has hopefully been dropping down in our industrialized countries since the introduction of both primary and secondary prevention. Nevertheless, it is still lethal in one out of two affected women though the introduction of cytological screening has dramatically reduced the mortality. Progressive diffusion of anti-HPV vaccination, the broadening of the viral types concerned, its association with existing screening measures and finally the introduction of viral detection as a screening tool must optimize the results already obtained.

  15. 宫颈液基细胞学检查在宫颈病变筛查中的临床应用%The clinical application of liquid based cytologic test on cervical carcinoma screening

    Institute of Scientific and Technical Information of China (English)

    董健余

    2011-01-01

    目的 探讨宫颈液基细胞学检查(TCT)技术在预防与筛查宫颈病变中的临床应用价值。方法 9 582例患者进行TCT检测,对阳性病例再进行阴道镜检查加组织活检。结果 9 582例标本中,异常涂片有392例,其中ASC 311例(3.24%),LSIL 45例(0.47%),HSIL 13例(0.13%),AGC 21例(0.22%),SCC 1例(0.01%)。在所有阳性病例中,35~39岁组人群的阳性检出率最高(20.92%),30~34岁组其次(20.66%)。TCT与组织活检检查的总体符合率为58.67%,ASC、LSIL、HSIL、AGC、SCC的符合率分别为54.98%、80.00%、100.00%、42.86%、100.00%。TCT对HSIL及以上的病变的阳性符合率明显高于LSIL及以下的病变,差异有统计学意义(t=9.810,P=0.002)。结论 TCT结合阴道镜下病理检查是筛查并及早发现宫颈癌的癌前病变的有效方法。%Objective To study the clinical application value of TCT technology in prevention and screening cervical lesions.Methods 9 582 cases received TCT detection. The positive patients received further biopsy vaginal endoscopy. Results In 9 582 specimens, there were 392 abnormal smear including 311 ASC (3.24%) ,45 LSIL (0.47%) ,13 HSIL(0. 13% ),21 AGC(0. 22% ),1 SCC(0. 01% ). The 35 ~39 age group had the highest positive rate(20. 92% ) and the 30~35 age group was prior(20. 66% ) in the positive group. The coordination rate between TCT and biopsy endoscopy was 58. 67%. The coordination rates for ASC, LSIL, HSIL, AGC, SCC were 54. 98% ,80. 00%, 100. 00% ,42. 86%, 100. 00% respectively. The effect of TCT on the positive coordination rate of upper HSIL was higher than lower HSIL( x2 =9. 810,P =0. 002). Conclusion The combination of TCT and biospy endoscope was an effective method to screen the early cervical cancer.

  16. Cerebrospinal fluid cytology studies of neuropsychiatric systemic lupus erythematosus

    Directory of Open Access Journals (Sweden)

    CHEN Lin

    2013-02-01

    Full Text Available Background Neuropsychiatric systemic lupus erythematosus (NP-SLE is the central nervous system (CNS involvement of systemic lupus erythematosus (SLE. The diagnosis of NP-SLE may be difficult due to the lack of specific biomarker. CNS cerebrospinal fluid (CSF cytology is diagnostic significant to CNS autoimmune disease. This paper described the characteristics of CSF cytology and evaluated its diagnostic value in NP-SLE. Methods Seventy-six eligible patients with clear diagnosis of NP-SLE were collected for CSF cytological examinations. Results The CSF cytology findings of 25 cases in 76 were abnormal, among which 16 cases showed lymphocytic inflammatory reactions; 8 cases had slight increase of neutrophile granulocyte percent; 9 cases showed lymphocyte-neutrophile inflammation. Activated lymphocytes together with monocytes were present in 24 cases. Among those cases, abnormal endocytosis of monocytes, which presented as monocytes phagocytosing lymphocytes or plasmocytes, was shown in 17 cases; plasmocytes were found in 17 cases. Conclusion The diagnosis of NP-SLE is based on clinical, neuroimaging and CSF studies. Among these methods, the CSF cytology findings are quite useful in practice, since the CSF cytological inflammatory reactions, especially the presentation of abnormal phagocytes in CSF is typical in NP-SLE and indicates its vasculitic mechanism.

  17. TCT、阴道镜组织病理学及 HPV 检查在宫颈病变诊断中的意义%The Value of Thinprep Cytology Test(TCT),Vaginoscope Histopathologic Examination and HPV-test in the Diagnosis of Cervical Disease

    Institute of Scientific and Technical Information of China (English)

    孙素玲; 王新云

    2012-01-01

      目的综合评价超薄液基细胞学(TCT)、阴道镜组织病理及人乳头状瘤病毒高危型(HR-HPV)检查在宫颈病变诊断中的应用价值.方法2007年1月~2010年12月期间,行 TCT 检查及健康查体者5836例,其中检出宫颈病变518例,对有宫颈病变患者进行阴道镜组织病理学检查,对 CINⅠ及以上的214例患者进行 HPV 检查.结果 TCT 与组织病理结果比较,符合率为86.3%.HPV 阳性率分别为 CINⅠ为19.4%(25/129),CINⅡ52.8%(28/53),CINⅢ及宫颈癌100%(32/32),随着宫颈组织病理学级别增高,HPV 感染阳性率明显增高.结论 TCT、阴道镜组织病理学及 HPV 检查联合应用可以提高宫颈病变的检出率,有利于早预防、早诊断、早治疗,达到有效防治宫颈癌的目的.%  Objective To evaluate the value of thinprep cytology test ,vaginoscope Histopathologic examination and HPV -test in the diagnosis of cervical disease .Methods In January 2007 to February 2010 period,5836 outpatients were given thinprep cytology Test (TCT) examination in Hospital.Five hundred and eighteen cases of cervical lesions were further underwent vaginoscope histopathologic exam -ination.Two hundred and fourteen cases of cervical intraepithelial neoplasia and cancer were examined for HR -HPV by PCR.Results Among the 518 cases,the coincidence between the findings of TCT and pathology was 86.3%.The positive rate of HR-HPV of CINⅠ,CINⅡ,CINⅢand cervical cancer was 19.4%(25/129),52.8%(28/53)and 100%(32/32) respectively.The positive rate HR-HPV infection was signifi-cantly raised with the increased level of cervical pathology .Obviously,HR-HPV examination in the diagnosis of cervical lesions is extremely important.Conclusion The combination of the above examination can increase the detection rate of lesions that can be beneficil to early pre -vention,early diagnosis and early treatment ,and effective prevention and treatment of cervical cancer .

  18. Cervical pre-malignant lesions in HIV infected women attending Care and Treatment Centre in a tertiary hospital, Dar es Salaam, Tanzania.

    Science.gov (United States)

    Balandya, Belinda S; Pembe, Andrea B; Mwakyoma, Henry A

    2011-09-01

    The aims of this study was to determine proportion of HIV infected women with cervical pre-malignant lesions; and compare the use of Visual Inspection of the cervix after application of Acetic acid (VIA) and Papanicolau (Pap) smear in screening for cervical premalignant lesions in HIV positive women attending Care and Treatment Centre (CTC) at Muhimbili National Hospital (MNH), Dar es Salaam, Tanzania. A total of 316 women aged 18-70 years had a Pap smear taken for cytology, followed by spraying onto the cervix with 4% acetic acid and then inspecting it. Cytology was considered negative when there was no Cervical Intraepithelial Neoplasia (CIN) lesion reported from the Pap smear taken, and positive if CIN lesion 1, 2 or 3 was reported. Detection of a well-defined, opaque acetowhite lesion close to the squamocolumnar junction or close to the external cervical os constituted a positive VIA. Out of 316 women, 132 women had acetowhite lesions on VIA, making the proportion of abnormal cervical lesions to be 42.4%. One hundred and one out of 312 women (32.4%) had CIN lesions detected on Pap smear. The proportion of agreement between these two tests was 0.3. The proportion of agreement was moderate in women with advanced WHO HIV clinical stage of the disease and in women not on ART (Anti Retroviral Therapy). Women with CD-4 count less than 200 cells/mm3 had more abnormal cervical lesions. There is considerable proportion of HIV positive women with premalignant lesions of the cervix. Considering the proportion of HIV women with abnormal lesions and the difficulty in logistics of doing Pap smear in low resource settings, these results supports the recommendation to introduce screening of premalignant lesions of the cervix using VIA to all HIV infected women.

  19. Four and a half year follow up of women with dyskaryotic cervical smears.

    Science.gov (United States)

    Fletcher, A; Metaxas, N; Grubb, C; Chamberlain, J

    1990-01-01

    OBJECTIVE--To determine the proportion of women with mild or moderate dyskaryosis in cervical smears who (a) progress to cervical intraepithelial neoplasia grade III or worse or (b) regress. DESIGN--Four and a half year cytological follow up study of women with mild or moderate dyskaryosis in cervical smears. SETTING--666 Women (mean age 28 (SD 8) years; range 14-74) found to have borderline, mild, or moderate dyskaryosis on routine screening. RESULTS--45 Women (6.8%) had a cone biopsy recommended on the basis of an abnormal follow up smear (severe dyskaryosis suggestive of cervical intraepithelial neoplasia grade III or invasive cancer), and in one patient cervical intraepithelial neoplasia grade III was reported in a biopsy specimen after dilatation and curettage. Life table analysis gave a 14% probability of a patient being recommended for a biopsy after four and a half years of follow up (95% confidence interval 12% to 15%). There was a significant excess incidence of invasive cancer of the cervix in the series compared with the general population (five cases observed compared with less than 0.1 expected). 157 Patients (24%) showed reversion to a normal cell pattern sustained in several smears over more than 18 months but a single negative smear was an unreliable indicator of apparent regression. Having two successive smears showing mild dyskaryosis or a smear at any time showing moderate dyskaryosis was a significant predictor of a subsequent severely dyskaryotic smear. CONCLUSIONS--Women found to have mild or moderate dyskaryosis in cervical smears should be kept under regular surveillance. The optimum management of these patients--by cytology or colposcopy--needs to be determined by randomised controlled trials. PMID:2224218

  20. Expenditure and resource utilisation for cervical screening in Australia

    Directory of Open Access Journals (Sweden)

    Lew Jie-Bin

    2012-12-01

    Full Text Available Abstract Background The National Cervical Screening Program in Australia currently recommends that women aged 18–69 years are screened with conventional cytology every 2 years. Publicly funded HPV vaccination was introduced in 2007, and partly as a consequence, a renewal of the screening program that includes a review of screening recommendations has recently been announced. This study aimed to provide a baseline for such a review by quantifying screening program resource utilisation and costs in 2010. Methods A detailed model of current cervical screening practice in Australia was constructed and we used data from the Victorian Cervical Cytology Registry to model age-specific compliance with screening and follow-up. We applied model-derived rate estimates to the 2010 Australian female population to calculate costs and numbers of colposcopies, biopsies, treatments for precancer and cervical cancers in that year, assuming that the numbers of these procedures were not yet substantially impacted by vaccination. Results The total cost of the screening program in 2010 (excluding administrative program overheads was estimated to be A$194.8M. We estimated that a total of 1.7 million primary screening smears costing $96.7M were conducted, a further 188,900 smears costing $10.9M were conducted to follow-up low grade abnormalities, 70,900 colposcopy and 34,100 histological evaluations together costing $21.2M were conducted, and about 18,900 treatments for precancerous lesions were performed (including retreatments, associated with a cost of $45.5M for treatment and post-treatment follow-up. We also estimated that $20.5M was spent on work-up and treatment for approximately 761 women diagnosed with invasive cervical cancer. Overall, an estimated $23 was spent in 2010 for each adult woman in Australia on cervical screening program-related activities. Conclusions Approximately half of the total cost of the screening program is spent on delivery of primary

  1. CCR2-V64I polymorphism is associated with increased risk of cervical cancer but not with HPV infection or pre-cancerous lesions in African women

    International Nuclear Information System (INIS)

    Cervical cancer, caused by specific oncogenic types of human papillomavirus (HPV), is the second most common cancer in women worldwide. A large number of young sexually active women get infected by HPV but only a small fraction of them have persistent infection and develop cervical cancer pointing to co- factors including host genetics that might play a role in outcome of the HPV infection. This study investigated the role of CCR2-V64I polymorphism in cervical cancer, pre-cancers and HPV infection in South African women resident in Western Cape. CCR2-V64I polymorphism has been previously reported to influence the progression to cervical cancer in some populations and has also been associated with decreased progression from HIV infection to AIDS. Genotyping for CCR2-V64I was done by PCR-SSP in a case-control study of 446 women (106 black African and 340 mixed-ancestry) with histologically confirmed invasive cervical cancer and 1432 controls (322 black African and 1110 mixed-ancestry) group-matched (1:3) by age, ethnicity and domicile status. In the control women HPV was detected using the Digene Hybrid Capture II test and cervical disease was detected by cervical cytology. The CCR2-64I variant was significantly associated with cervical cancer when cases were compared to the control group (P = 0.001). Further analysis comparing selected groups within the controls showed that individuals with abnormal cytology and high grade squamous intraepitleial neoplasia (HSIL) did not have this association when compared to women with normal cytology. HPV infection also showed no association with CCR2-64I variant. Comparing SIL positive controls with the cases showed a significant association of CCR2-64I variant (P = 0.001) with cervical cancer. This is the first study of the role of CCR2-V64I polymorphism in cervical cancer in an African population. Our results show that CCR2-64I variant is associated with the risk of cervical cancer but does not affect the susceptibility to HPV

  2. TCT、HPV、阴道镜下活体组织检查及LEEP术在宫颈疾病筛查中的临床价值%Clinical values of Thin Prep cytologic test, human papillomavirus detection, biopsy under colposcope and LEEP in screening of cervical diseases

    Institute of Scientific and Technical Information of China (English)

    景笑笑; 杨雪峰

    2012-01-01

    目的:以病理组织学为确诊标准探讨TCT、HPV、阴道镜下活体组织检查(活检)及LEEP术在宫颈疾病筛查中的临床价值.方法:收集2010年1月~2011年5月于郑州大学第三附属医院妇科门诊行阴道镜下活检的989例患者,比较其TCT、HPV及组织病理学结果,对TCT与组织学结果不符的114例进一步行LEEP手术并送病理.结果:TCT诊断宫颈疾病的敏感性、特异性、阳性预测值、阴性预测值、漏诊率分别为91.32%、68.78%、42.98%、97.01%、5.12%.HPV的阳性检出率64.65%,感染率与病理级别呈正相关,HPV负荷量在宫颈上皮内瘤变明显高于宫颈炎,差异有统计学意义(P<0.05).阴道镜下活检与LEEP术后病理、级别下降30例,上升44例.结论:TCT联合HPV- DNA检测能提高宫颈病变的检出率,HPV -DNA的定量检测有助于细胞学阳性患者的分流,LEEP术可在一定程度上弥补活检的不足,提高宫颈病变的诊断率.%Objective: To explore the clinical values of Thin Prep cytologic test (TCT) , human papillomavirus (HPV) detection, biopsy under colposcope, and loop electrosurgical excision procedure (LEEP) in screening of cervical diseases taking histopathology as diagnostic criterion. Methods: A total of 989 patients who underwent biopsy under colposcope in gynecological outpatient of the hospital from January 2010 to May 2011 were collected, the results of TCT, HPV detection, and histopathology were compared, 114 patients whose results of TCT and histology disagreed received LEEP and pathological examination. Results: The sensitivity, specificity, positive predictive value, negative predictive value, and missed diagnostic rate of TCT in diagnosis of cervical diseases were 91. 32% , 68. 78% , 42. 98% , 97. 01% , and 5. 12% , respectively. The positive detection rate of HPV was 64. 65%. There was a positive correlation between HPV infection rate and pathological grades; HPV load in cervical intraepithelial

  3. The correlation between HIV seropositivity, cervical dysplasia, and HPV subtypes 6/11, 16/18, 31/33/35

    DEFF Research Database (Denmark)

    Tweddel, G; Heller, P; Cunnane, M;

    1994-01-01

    Twenty-one human immunodeficiency virus (HIV) seropositive patients were studied to determine the prevalence of cervical dysplasia, the distribution of human papilloma virus (HPV) subtypes, and the utility of cytologic diagnosis in this population. The patients ranged in age from 18 to 41 years...... abnormalities. In 14 patients there was sufficient tissue for HPV typing by in situ hybridization; the HPV subtypes identified were 6/11 (10 patients), 16/18 (9 patients), and 31/33/35 (9 patients). Dysplasia was present in 11/14 (79%) of the specimens submitted for subtyping. The absolute CD4 cell counts were...

  4. 液基薄层细胞学检查与阴道镜活检在宫颈癌诊断中的应用对比研究%Application of liquid based thin-layer cytology and colposcopy biopsy in the diagnosis of cervical cancer

    Institute of Scientific and Technical Information of China (English)

    徐继勋; 熊娟

    2015-01-01

    目的:探讨液基薄层细胞学检查与阴道镜活检在宫颈癌诊断中的临床诊断效果。方法:选取2013年4月至2014年4月在我院妇科门诊接受宫颈癌筛查的1434患者为研究对象,对采取宫颈液基薄层细胞学检查(TCT)后结果呈阳性的202例患者行阴道镜下宫颈活检,对比诊断结果。结果:参与本次宫颈癌筛查的1434患者中,病理检验正常或炎症者1200例,阴性率为837%,检测结果呈阳性者234例,阳性率为163%;TCT检查结果呈阳性的患者202例,阳性检出率为863%,其中诊断结果为ASC-US、LSIL、HSIL或SCC者分别125例(619%)、53例(262%)、20例(99%)和4例(20%),两组检测方法对比无明显差异,P>005;阴道镜下活检结果与TCT检查结果相符率分别为SCC25%(1/4)、HSIL60%(12/20)、LSIL66%(35/53);ASC-US结果中,阴道镜下活检CINⅠ48例、CINⅡ7例、CINⅢ3例,共58例,占例数的464%。结论:液基薄层细胞学检查能有效提升宫颈癌及宫颈上皮内病变(CIN)等癌前病变检出率,对宫颈癌早期诊断意义重大。对于TCT检查结果为无明确意义的非典型细胞改变(ASC-US)患者,应引起临床重视,根据患者病情行阴道镜检查,以提升宫颈癌及癌前病变诊断准确率,争取宝贵的治疗时间。%Objectives:To investigate the diagnostic effect of liquid thin -layer cytology and colposcopy bi-opsy in the diagnosis of cervical cancer.Method:1434 patients received cervical cancer screening selection from A-pril 2013 -April 2014 in outpatient of gynaecology in our hospital were selected as the research objects.202 pa-tients with a positive result of cervical liquid thin -layer cytology (TCT)received cervical biopsy under colposcopy to compare the diagnosis results.Results:Among the 1434 patients received cervical cancer screening,there were 1200

  5. Correlation between cervical curvature abnormality and symptoms of adolescent headache%颈椎曲度异常与青少年头痛等症状的相关性研究

    Institute of Scientific and Technical Information of China (English)

    常蜀英; 张国强; 张卫清; 陈勇; 黎建民; 李申生

    2004-01-01

    度异常源性头痛伴随症状中以头晕(81例)最多见,其中记忆障碍21例,晕厥发作17例和其他症状39例,3组间积分差异显著(P<0.05~0.01).3组患者中Hoffmann征(40例)和Romberg征(58例)阳性积分比较差异显著(P<0.05~0.01).随颈椎曲度异常加重,120例患者中血压呈上升趋势,收缩期血压3组分别为(108.6±13.1),(112.6±13.4)和(118.5±17.9)mmHg(1mmHg=0.133 kPa),组间比较差异显著(F=4.374,P=0.015),舒张期血压3组分别为(72.6±6.8),(77.1±9.5)和(78.8±10.2)mmHg,组间比较差异显著(F=5.219,P=0.007).颈曲异常各组的脑电图和经颅血管彩色超声检查比较差异显著(F=8.243,P<0.05和F=11.983,P<0.01),重度颈曲异常组阳性积分值最高.疗效分析:100例接受治疗的患者中9例因路途远,学习忙而中断治疗,其余91例中,痊愈55例(60%),显效22例(24%),好转9例(10%),无效加重5例,总有效率95%;曲度异常轻、中、重三组间疗效差异不显著(X2=2.865,P=0.239).结论:颈椎曲度异常与青少年头痛的异常临床症状、体征密切相关,与脑电图、TCD检查异常有关,可能是青少年颈源性头痛一个不可忽视的潜在病因;牵引、按摩的物理治疗对减轻此类头痛有效.%BACKGROUND: The detective rate of abnormal cervical curvature in adolescent patients with headache is comparatively high, which suggests that cervical curvature abnormality may be the potential pathogenesis inducing the clinical symptoms of cervicogenic headache(CEH) and dizziness. The correlation between cervical curvature abnormality and the clinical symptoms of adolescent headache requires thorough researches.OBJECTIVE: To investigate the correlation between cervical curvature abnormality and the clinical symptoms and physical signs of adolescent headache for the exploration of effective therapeutic methods.DESIGN: A case-controlled trial based on diagnosis.SETTING and PARTICIPANTS: A total of 120 patients including 43 male and 77 female

  6. Agreement Between Visual Inspection with Acetic Acid and Papanicolaous Smear as Screening Methods for Cervical Cancer

    International Nuclear Information System (INIS)

    Objective: To determine degree of agreement between visual inspection with acetic acid (VIA) and Papanicolaous (Pap) smear as screening methods for cervical cancer. Study Design: A cross-sectional study. Place and Duration of Study: Department of Obstetrics and Gynaecology, Sir Ganga Ram Hospital, Lahore, from July to December 2012. Methodology: Two hundred and fifty women in reproductive age group presenting with various gynaecological complaints were included in the study. A Papanicolaous smear was taken and visual inspection with 5% acetic acid was done. VIA was reported as positive or negative according to acetowhite changes and cytology result was graded as CIN 1, 2, 3 and squamous carcinoma. Those women who showed positive result with either VIA or Pap smear or both were further subjected to colposcopic directed biopsy which was taken as gold standard. Results were computed using Statistical Package for Social Sciences (SPSS) version 16 and statistical test used was kappa. Results: Out of 250 women, VIA was positive in 55 (22%) patients and Pap smear was abnormal in 27 (10.8%). Histological diagnosis of CIN/cancer was made in 36 out of a total 62 patients who underwent biopsy. Conclusion: There was a fair agreement between VIA and Pap smear, with VIA detecting more abnormalities than cytology. In the absence of Pap smear availability, VIA may be a reasonable cervical cancer screening method, especially in low resource settings. (author)

  7. Human papillomavirus detection from human immunodeficiency virus-infected Colombian women's paired urine and cervical samples.

    Directory of Open Access Journals (Sweden)

    Marina Munoz

    Full Text Available Infection, coinfection and type-specific human papillomavirus (HPV distribution was evaluated in human immunodeficiency virus (HIV-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204 were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R. HPV-positive samples were typed for six high-risk HPV (HR-HPV (HPV-16, -18, -31, -33, -45 and -58 and two low-risk (LR-HPV (HPV-6/11 types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine followed by HPV-31(47.2% in cervical samples and HPV-58 (35.7% in urine samples. There was 55.4% coinfection (infection by more than one type of HPV in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in 25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance.

  8. Human papillomavirus detection from human immunodeficiency virus-infected Colombian women's paired urine and cervical samples.

    Science.gov (United States)

    Munoz, Marina; Camargo, Milena; Soto-De Leon, Sara C; Sanchez, Ricardo; Parra, Diana; Pineda, Andrea C; Sussmann, Otto; Perez-Prados, Antonio; Patarroyo, Manuel E; Patarroyo, Manuel A

    2013-01-01

    Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV) (HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine) followed by HPV-31(47.2%) in cervical samples and HPV-58 (35.7%) in urine samples. There was 55.4% coinfection (infection by more than one type of HPV) in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in 25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance.

  9. Cervical screening and cervical cancer death among older women: a population-based, case-control study.

    Science.gov (United States)

    Rustagi, Alison S; Kamineni, Aruna; Weinmann, Sheila; Reed, Susan D; Newcomb, Polly; Weiss, Noel S

    2014-05-01

    Recent research suggests that cervical screening of older women is associated with a considerable decrease in cervical cancer incidence. We sought to quantify the efficacy of cervical cytology screening to reduce death from this disease. Among enrollees of 2 US health plans, we compared Papanicolaou smear screening histories of women aged 55-79 years who died of cervical cancer during 1980-2010 (cases) to those of women at risk of cervical cancer (controls). Controls were matched 2:1 to cases on health plan, age, and enrollment duration. Cytology screening during the detectable preclinical phase, estimated as the 5-7 years before diagnosis during which cervical neoplasia is asymptomatic but cytologically detectable, was ascertained from medical records. A total of 39 cases and 80 controls were eligible. The odds ratio of cervical cancer death associated with screening during the presumed detectable preclinical phase was 0.26 (95% confidence interval: 0.10, 0.63) after adjustment for matching characteristics, smoking, marital status, and race/ethnicity using logistic regression. We estimate that cervical cytology screening of all women aged 55-79 years in the United States could avert 630 deaths annually. These results provide a minimum estimate of the efficacy of human papillomavirus DNA screening-a more sensitive test-to reduce cervical cancer death among older women.

  10. Cervical dysplasia

    Science.gov (United States)

    ... by your provider. Make sure to get the HPV vaccine when it is offered to you. This vaccine prevents many cervical cancers. ... Early diagnosis and prompt treatment cures most cases of cervical ... severe cervical dysplasia may change into cervical cancer.

  11. Distribution of HPV genotypes in cervical intraepithelial lesions and cervical cancer in Tanzanian women

    Directory of Open Access Journals (Sweden)

    Vidal Adriana C

    2011-11-01

    Full Text Available Abstract Background Infection with human papillomavirus (HPV is associated with uterine cervical intraepithelial neoplasia (CIN and invasive cancers (ICC. Approximately 80% of ICC cases are diagnosed in under-developed countries. Vaccine development relies on knowledge of HPV genotypes characteristic of LSIL, HSIL and cancer; however, these genotypes remain poorly characterized in many African countries. To contribute to the characterization of HPV genotypes in Northeastern Tanzania, we recruited 215 women from the Reproductive Health Clinic at Kilimanjaro Christian Medical Centre. Cervical scrapes and biopsies were obtained for cytology and HPV DNA detection. Results 79 out of 215 (36.7% enrolled participants tested positive for HPV DNA, with a large proportion being multiple infections (74%. The prevalence of HPV infection increased with lesion grade (14% in controls, 67% in CIN1 cases and 88% in CIN2-3. Among ICC cases, 89% had detectable HPV. Overall, 31 HPV genotypes were detected; the three most common HPV genotypes among ICC were HPV16, 35 and 45. In addition to these genotypes, co-infection with HPV18, 31, 33, 52, 58, 68 and 82 was found in 91% of ICC. Among women with CIN2-3, HPV53, 58 and 84/83 were the most common. HPV35, 45, 53/58/59 were the most common among CIN1 cases. Conclusions In women with no evidence of cytological abnormalities, the most prevalent genotypes were HPV58 with HPV16, 35, 52, 66 and 73 occurring equally. Although numerical constraints limit inference, findings that 91% of ICC harbor only a small number of HPV genotypes suggests that prevention efforts including vaccine development or adjuvant screening should focus on these genotypes.

  12. Reflections on the ACS-ASCCP-ASCP Cervical Cancer Screening Guidelines 2012%美国最新子宫颈癌筛查指南学习与思考

    Institute of Scientific and Technical Information of China (English)

    孙丽芳

    2015-01-01

    Cervical cancer screening has become a widely accepted practice to reduce the incidence of cervical cancer. Standardized protocols of screening would help prevent the failure of diagnosis and therapy. ACS-ASCCP-ASCP Cervical Cancer Screening Guidelines 2012 recommends standardized screening methods for females of different ages with different types of abnormal cervical cytology. Specifically, it states that the joint screening on cervical cytology and HPV (co-test) is the most effective practice for females of 30 years old and older.%虽然子宫颈癌筛查已较为普及,但规范诊治仍有待进一步加强。美国2012子宫颈癌筛查指南细化了不同年龄各种宫颈细胞学异常的处理原则,特别强化了宫颈细胞学+人乳头状瘤病毒(HPV)联合检测是30岁以上女性最好的筛查方法。

  13. Accuracy of liquid-based cytology in diagnosis of high-grade squamous cervical intraepithelial neoplasia%影响宫颈液基细胞学高度鳞状上皮内病变诊断的因素分析

    Institute of Scientific and Technical Information of China (English)

    李旻; 梅平; 罗东兰; 王小冰; 刘艳辉

    2012-01-01

    目的 探讨影响宫颈液基细胞学高度鳞状上皮内病变(HSIL)诊断准确性的原因.方法 收集2007至2010年间415例细胞学诊断为HSIL的病例,将细胞学结果与组织学结果对照分析,复查液基细胞学制片及组织学切片,分析误诊原因.结果 共42209例受检者进行了ThinPrep液基细胞学检查,其中细胞学诊断为HSIL者415例,占筛查人群的1.0%.HSIL患者的平均年龄41.6岁,30~ 49岁为高发年龄.325例细胞学诊断为HSIL的病例其组织学诊断结果为:阴性/炎性改变23例(7.1%),宫颈上皮内病变(CIN)1级/人乳头状瘤病毒(HPV)感染22例(6.8%),CIN2级/CIN3级223例(68.6%),鳞状细胞癌(SCC)57例(17.5%).细胞学HSIL对组织学CIN2级及以上病变的阳性预测值为86.2% (280/325),对组织学CIN1级以上病变的阳性预测值为92.9%(302/325).细胞学假阳性的原因主要是组织学取材阴性、细胞学片中出现反应性腺上皮、孤岛状萎缩及放化疗反应等.细胞学诊断为HSIL而组织学诊断为SCC者57例(17.5%),误诊的原因主要为社会因素、对部分低分化SCC的细胞学表现认识不足,以及细胞学片中缺乏典型癌性特征.结论 细胞学HSIL对宫颈CIN2级/CIN3级及SCC的阳性预测值较高,但存在与组织学诊断结果不符的情况,细胞学医师和妇科医师应注意避免造成不一致的因素.%Objective To investigate factors affccting the diagnostic accuracy of cervical liquidbased cytology for high-grade squamous intraepithelial lesion (HSIL).Methods A retrospective evaluation of cytological and histological slides was performed in 415 patients who had cytological HSIL between 2007and 2010.Results Among 42 209 cases screened by ThinPrep liquid-based cytology,415 cases ( 1.0% )of HSIL were eventually identified.The mean age of HSIL patients was 41.6 years,and 30 -49 years were the most common age group.Among 415 cases,325 patients had available histological diagnosis as follows

  14. HPV检测与细胞学检测在宫颈病变诊断中的应用%Comparison and application of high-risk human papillomavirus DNA test and thinprep liquid based cytology for the screening of cervical lesions

    Institute of Scientific and Technical Information of China (English)

    刘彩艳; 曲芃芃; 陈红晓

    2011-01-01

    Objective: To evaluate the significance of high-risk human papillomavirus (HPV) DNA test and thinprep liquid based cytology for the screening of cervical lesions. Methods: 1 036 women were monitored by thinprep liquid based cytology and hybrid capture II, taking biopsy pathology result as standard. Results: In 1 036 women, by thinprep liquid based cytology, normal and benign reactive hy-perplasia were found in 575, 281 women, ASCUS, LSIL and HSIL were found in 72, 55, 53 women. 376 cases were positive of HR-HFV DNA. By biopsy under the celposeope, 72, 6land 65 women were diagnosed as C1N I, CIN II and CINl, respectively, while 11 women were diagnosed as cervical cancer. The sensitivity, specificity, positive predictive values and negative predictive values of the thinprep liquid based cytology were 63.16%, 94,19%, 73.3% and 91.0%. The sensitivity, specificity, positive predictive values and negative predictive values of HR-HPV were 5.28%, 75.69%, 47.0% and 85.17%. Additionally, the sensitivity, specificity positive predictive values and negative predictive values were 98.65%, 97.21%, 89.59% and 94.73% by HR-HPV combined thinprep liquid based cytology. Conclusion: Combining thinprep liquid based cytology with HR-HPV DNA test may increase the sensitivity and specificity for detecting cervical lesions.%目的:评价高危型人乳头状瘤病毒(HPV)DNA检测联合宫颈液基细胞学检查在宫颈病变筛查中的价值.方法:采用宫颈超薄液基细胞学(TCT)检洲及第二代杂交捕获试验(HC-2)对1036例患者进行宫颈机会筛查,以宫颈组织病理学检查结果作为确诊的标准.结果:细胞学检查发现正常575例;良性反应性改变281例;不典型鳞状细胞72例;低度鳞状上皮内病变55例;高度鳞状上皮内病变53例.HR-HPV感染376例,阳性率36.29%;经组织病理检查确诊宫颈上皮内瘤变(CIN) I72例,CINⅡ61例,CINⅢ65例,宫颈癌11例.高危型HPV-DNA在宫颈病变组与正常组比较有显

  15. Bax and Bak expression in cervical smears of women with low-and high-risk HPV types: A study of 120 cases

    Directory of Open Access Journals (Sweden)

    Eirini Klapsinou

    2015-01-01

    Full Text Available Background: Human papillomavirus (HPV is known to be involved in the carcinogenesis of squamous cells in uterine cervix cancer, mostly by binding and inactivating the p53 and pRb tumor suppressor genes. Lately, evidence has emerged suggesting that HPV oncoproteins may interact with proteins involved in cellular apoptosis as well. Aim: This study aimed to investigate the expression of proapoptotic proteins Bax and Bak in women with low-risk and high-risk HPV types as opposed to HPV-negative women, and in women with normal pap smear compared to women with abnormal Papanicolau test (Pap smear. Materials and Methods: A total of 120 liquid-based cervical samples were subtyped for HPV types with microarray hybridization and then stained and evaluated immunocytochemically for Bax and Bak expression. Statistical analysis was performed on the Bax and Bak scores (percentage of positive cells × staining intensity, the overall percentage of positive cells, and the most prevalent staining intensity group found in each sample. Results: A weak association between negative Bax staining and cytologically normal Pap smears was discovered, whereas cytologically abnormal samples tended to stain weakly or moderately positive. No other statistically significant difference was found in the other analyzed parameters. Conclusion: Cytologically normal pap smears seem to have a slight tendency to stain negative for Bax as opposed to cytologically abnormal pap smears. Although the association is weak, it is an indication that there might be a connection between the expression of Bax and the development of cervical intraepithelial dysplasia, which warrants further investigation in larger-scale studies.

  16. Incidence of cervical dysplasia and cervical cancer in women living with HIV in Denmark

    DEFF Research Database (Denmark)

    Thorsteinsson, Kristina; Ladelund, Steen; Jensen-Fangel, Søren;

    2014-01-01

    INTRODUCTION: Women living with HIV (WLWH) are reportedly at increased risk of invasive cervical cancer (ICC). WLWH in Denmark attend the National ICC screening program less often than women in the general population. We aimed to estimate the incidence of cervical dysplasia and ICC in WLWH...... and hazard ratios (HRs) for time from inclusion to first cervical intraepithelial neoplasia (CIN)/ICC and time from first normal cervical cytology to first CIN/ICC were estimated. Sensitivity analyses were performed to include prior screening outcome, screening intensity and treatment of CIN...... with normal baseline cytology, incidences of CIN1+ and CIN2+ were higher in WLWH. However, incidences were comparable between WLWH and controls adherent to the National ICC screening program. CONCLUSIONS: Overall, WLWH develop more cervical disease than controls. However, incidences of CIN are comparable...

  17. Cervical intraepithelial lesions in females attending Women′s Health Clinics in Alexandria, Egypt

    Directory of Open Access Journals (Sweden)

    Mona Abdel-Hadi

    2015-01-01

    Full Text Available Background: Data from Egyptian studies provide widely varying estimates on the prevalence of preinvasive cervical lesions. The aim of this study was to estimate the rate of cervical intraepithelial neoplasia (CIN   in Egyptian women living in Alexandria to clarify the need for implementing a national organized screening program and a vaccination program in our community. Materials and Methods: The study was conducted over a 6 years period and covered the different socioeconomic levels to have a representative sample for women living in Alexandria. All women included did not have any cervical disorder related complaints. Conventional Pap smears were obtained and diagnosed using the Bethesda system. Women with abnormal Pap smears were managed according to the 2006 consensus guidelines within the available facilities. Persistent abnormal cytological results were referred for colposcopic biopsy. Histological results were grouped into: Reactive changes, CIN 1, CIN 2/CIN 3 and adenocarcinoma in-situ (AIS. Results: Out of the 6173 smears included in the study 6072 (98.36% were normal and only 101 (1.63% were abnormal. After colposcopic biopsies, 0.08% had CIN 1, 0.03% had CIN 2, 3 and 0.01% had AIS. Conclusion: We concluded that cervical cancer screening programs, although life-saving for a number of women, are not a sufficiently high priority in our community. Money for national health screening programs should preferably be directed more towards recruiting women for breast cancer screening, since breast cancer accounts for about 33% of all female cancers in Egypt ranking number one, while cervical cancer ranks number 13.

  18. The LTS liquid based cytology producer joint residue fluid HPV 16/18 in early cervical cancer screening test application value%LTS液基细胞学联合HPV16/18检测筛查早期宫颈癌的价值

    Institute of Scientific and Technical Information of China (English)

    兰淼; 李艳红; 朱少君; 巩丽; 韩秀娟; 任拼; 姚丽; 张伟

    2011-01-01

    Objective > Explore the LTS liquid based cytology producer joint liquid based residual liquid HPV16/18 cervical cancer screening lesion before value of clinical application. Method t Using the LTS liquid based cells producer method to detect 1000 cases of patients with the mouth and obstetrics and gynecology cervical neck tube exfoliated cells and discharge,the remaining liquid based cells remain fluid HPV16/18 immune markmethod to detect. HPV16/18 positive and highly suspected patients with cervical lesions vaginal microscopically more biopsy, combined with the pathologic result analysis cervical lesions. Results: 1000 cases of sample analysis by the LTS detection in 870, 24 cases of ASCUS ASCUS-H 26 cases, LSIL 61 cases, H SIL 19 cases. 130 cases of vaginal cytology unusual person under mirror more tissue biopsies confirmed 71 cases of inflammatory, CINI18 example, CINII 21 example, CINIII 13 cases, invasive cancer in 3, 4 cases were wet wart. Immune markmethod to detect DNA found HPV16/18 infection rates for: normal or inflammation respectively 21. 1%, CINI16. 7 %, CINII 33. 3%, CINIII 69.2%, invasive cancer, wet wart 40.0% 100%. 65 cases of normal, HPV16/18 LTS positive patients with pathological test showed that inflammation 51 cases, CINI (19 cases), CINII5 cases. The LTS normal or inflammation and HPV16/18 positive patients with cervical lesions, the detection rate of 21. 5%. Conclusion: Liquid based cytology producer joint liquid based residual liquid HPV16 /18 cells detection can add cytology results, the screening early cer ical cancer to have the important meaning.%目的:探讨LTS液基细胞学联合液基细胞残留液HPV16/18检测筛查宫颈癌前病变的临床应用价值.方法:采用LTS液基细胞制片法检测1000例患者宫颈外口及颈管脱落细胞和分泌物,将剩余液基细胞残留液进行HPV16/18免疫印记法检测.HPV16/18阳性且高度怀疑宫颈病变患者进行阴道镜下多点活检,结合病理

  19. Short-term risk of cervical intraepithelial neoplasia grades 2 and 3 for women with normal cytology and human papillomavirus infection Riesgo a corto plazo de lesiones intraepiteliales cervicales grados 2 y 3 en mujeres con citología vaginal normal e infección por el virus del papiloma humano

    Directory of Open Access Journals (Sweden)

    Gustavo Hernández-Suárez

    2010-12-01

    Full Text Available OBJECTIVE. To assess the risk of cervical intraepithelial neoplasia grades 2, 3 or higher (CIN 2/3+ for women with normal cytology and concurrent high-risk human papillomavirus infection (HR-HPV. MATERIAL AND METHODS. We examined 2 200 women every 6 months for an average of 9 years. Cervical smears and samples for HPV DNA were obtained at each visit. Absolute risk of subsequent CIN2/CIN3+ was estimated using the Kaplan-Meier method. RESULTS. The absolute risk of CIN2/CIN3+ among HR-HPV-positive women with normal Pap smear results was 1.06% (95%CI, 0.57-2.20, 5 times higher the risk among all women with normal Pap smears (0.20%; 95%CI, 0.12-0.32 but 7 times lower than that for women with HR-HPV infection and LSIL (7.24%; 95%CI, 3.78-15.2. CONCLUSION. Short-term absolute risk of CIN2/3+ after a normal Pap smear with concurrent HR-HPV infection is low (~1%, suggesting that the HR-HPV test has limited utility in short-term clinical decision-making for women with normal cytology.OBJETIVO. Evaluar el riesgo a corto plazo de neoplasia intraepitelial cervical de alto grado (CIN2/CIN3+ en mujeres con citologí-a cervicouterina normal e infección por virus del papiloma humano de alto riesgo (HR-HPV. MATERIAL Y MÉTODOS. Cohorte prospectiva de 2200 mujeres evaluadas cada seis meses durante 9 años en promedio. En cada visita se tomó muestra cervical para extendido y detección de HPV DNA. El riesgo absoluto de CIN2/CIN3+ a la siguiente visita fue calculado utilizando el método de Kaplan-Meier. RESULTADOS. En mujeres con citologí-a normal e infección concomitante por HR-HPV el riesgo absoluto de presentar CIN2/CIN3+ fue de 1.06% (95%CI, 0.57-2.20. Este riesgo fue cinco veces mayor al observado en todas las mujeres con citologí-a normal (0.20%; 95%CI, 0.12-0.32 pero siete veces menor que el observado en mujeres con lesiones intraepiteliales escamosas de bajo grado con infección concomitante (7.24%; 95%CI, 3.78-15.2. CONCLUSIÓN. El riesgo absoluto de

  20. Human papillomavirus types distribution in eastern Sicilian females with cervical lesions. A correlation with colposcopic and histological findings.

    Science.gov (United States)

    Le Donne, Maria; Giuffrè, Giuseppe; Caruso, Carmela; Nicotina, Piero Antonio; Alibrandi, Angela; Scalisi, Rosalba; Simone, Angela; Chiofalo, Benito; Triolo, Onofrio

    2013-07-01

    To determine human papillomavirus (HPV) types distribution in cervical lesions in a Southern Italian female population in Messina and their relationship between HPV type and grade of colposcopic and histopathological abnormality, a total of 253 women aged 17-68 years, with previous cytological abnormalities, were included in this study. HPV-DNA testing, colposcopy and biopsy were performed. For each sample, cervical cells were collected by centrifugation and DNA was extracted, followed by a PCR-based HPV-DNA assay and reverse dot blot genotyping. HPV-16 was found the most common type (46.6 %) followed by HPV-31 (26.9 %), -6 (18.6 %), -58 (8.8 %), -18 (6.7 %), -66 (5.7 %), -52 and -53 (4.7 %). Out of 62 women with abnormal transformation zone (ATZ) area compatible with squamous intraepithelial lesion (SIL) or cervical cancer (CC), 64.5 % was found high risk (HR) HPV-positive. Moreover the severity of the colposcopic diagnosis was positively correlated with the higher HPV oncogenicity risk (HPV-16 P = 0.023; and HPV-53 P = 0.047). The HPV-16 was found the most prevalent type within each histological category: 66.7 %, 31.2 %, 44 % and 37.2 % of CC, high grade (H)SIL, low grade (L)SIL and chronic cervicitis respectively; followed by HPV-31 present in 25 %, 8 %, and 13.3 % of HSIL, LSIL and chronic cervicitis respectively. A higher HPV incidence than the rest of Italy was found, in agreement with that detected by other authors for the South of the country. These data provide further information about the types prevalence in women with cervical lesions living in Eastern Sicily, suggesting the introduction of new targeted vaccines against a wider spectrum of HPV.

  1. The role of HPV in diagnosis and management of cervical premalignancies

    NARCIS (Netherlands)

    Hamont, D. van

    2008-01-01

    Cervical cytological pathology is not uncommon. Prevention of cervical cancer by detection of the disease in an early and pre-malignant stage is practised globally either through population-based screening programmes or more optimistically non-organised ones. High-grade cervical intraepithelial neop

  2. Patients with cervical cancer: why did screening not prevent these cases?

    NARCIS (Netherlands)

    Bie, R.P. de; Vergers-Spooren, H.C.; Massuger, L.F.A.G.; Siebers, A.G.; Salet-van der Pol, M.R.; Vedder, J.E.M.; Melchers, W.J.G.; Bulten, J.; Bekkers, R.L.M.

    2011-01-01

    OBJECTIVE: The objective of the study was to assess the screening history of women with cervical cancer and review normal cervical smears 5 years preceding the diagnosis. STUDY DESIGN: Cytological and histological results of 401 women treated for invasive cervical cancer between 1991 and 2008 at the

  3. Liquid-based cytology combined HPV-DNA genotyping in cervical cancer screening%液基细胞学检查联合人乳头瘤病毒基因分型检测在宫颈癌筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    刘汉忠; 杨继洲; 肖兰; 卢叶; 戴一菲; 王岚

    2013-01-01

    目的 探讨液基细胞学检查(TCT)联合人乳头瘤病毒基因(HPV-DNA)分型检测在宫颈癌筛查中的应用价值.方法 对28 524例女性患者进行TCT检测,其中5 932例TCT诊断为不能明确意义的非典型鳞状细胞(ASCUS)及以上的患者均行HPV-DNA分型检测及宫颈活检,分析TCT和HPV感染与宫颈癌及癌前病变的相关性.结果 共检测样本28 524例,其中良性病变22 592例(79.20%),ASCUS 4 824例(16.91%),鳞状上皮内低度病变(LSIL) 776例(2.72%),鳞状上皮内高度病变(HSIL) 316例(1.11%),鳞状细胞癌(SCC) 16例(0.06%).HSIL符合率为97.47%(308/316例),SCC符合率为100%(16/16例).HPV-DNA分型检测5 932例,其中阳性1 486例,阳性率为25.05%.TCT诊断与活检结果比较,良性病变与CINⅠ、CINⅡ、CINⅢ比较差异有统计学意义(P<0.01); CINⅠ、CINⅡ、CINⅢ、SCC间比较差异有统计学意义(P<0.01).宫颈活检结果与HPV感染在炎症及CINⅠ、CINⅡ、CINⅢ、SCC组间比较差异有统计学意义(P<0.01).结论 TCT与活检,特别是针对高级病变及癌,符合率较高,特异性较强.TCT与HPV-DNA分型检测对诊断宫颈癌及癌前病变具有敏感、特异和早于形态学改变的特点,具有较高的临床应用价值和推广前景.%Objective To investigate the value of the liquid-based cytology (TCT) joint HPV-DNA genotyping in cervical cancer screening.Methods Liquid-based cytology (TCT) combined HPV-DNA genotyping,and TCT were detected in 28 524 women,including 5 932 of TCT cytological diagnosis of ASCUS or more patients underwent genotyping of HPV-DNA detection and cervical biopsy,TCT and HPV infection and cervical cancer and precancerous lesions to cervical biopsy results confirmed standard.Results The total number of samples tested were 28 524 cases.Benign reactive changes were 22 592 cases (79.20%),ASCUS 4 824 cases (16.91%),LSIL 776 cases (2.72 %),HSIL 316 cases (1.11%),and the SCC 16 cases (0.06 %),in which

  4. Utility of Papanicolaou test in diagnosis of cervical lesions: a study in a tertiary care centre of western Uttar Pradesh

    Directory of Open Access Journals (Sweden)

    Mithila Bisht

    2015-05-01

    Full Text Available Background: Cervical cancer is the most common cancer among females in India. It is also one of the major causes of deaths among them. Papanicolaou (Pap cytological test helps in detecting the early epithelial abnormalities in cervical cells. The morbidity and mortality due to cervical cancer has come down in countries with well-established screening programmes at national level. This study was conducted to emphasize the value of cervical screening as a tool for early detection of non-neoplastic and neoplastic lesions of cervix. Slide positivity rate was computed and clinico-pathological correlation was done. Methods: This cross-sectional study was conducted from January 2012 to December 2014 (3 years, on 2202 women aged between 20-70 years coming for a Pap smear examination in a tertiary care hospital in Bareilly, India. After staining with conventional Papanicolaou technique, all cases were classified as per Bethesda nomenclature (2001. Results: Out of 2202 cases, 85.16% slides were found to be negative for intraepithelial abnormalities and 14.84% slides were labelled as positive for epithelial lesions. 97.67% of high grade lesions were found in women more than 40 years. Smears with epithelial cell abnormality were found more common in older age groups whereas smears negative for epithelial cell abnormality were found more common in young age groups. Conclusion: Pap test is a cost effective cancer screening and is a simple method to detect various lesions of cervix, non-neoplastic as well as neoplastic. High risk screening programmes should be directed to all women >40 years. Pap test is especially useful to diagnose precancerous lesions of cervix, thereby early detection of these lesions and subsequent proper treatment can be helpful in prevention of cervical cancer. [Int J Res Med Sci 2015; 3(5.000: 1070-1076

  5. Evaluation on the application value of Liquid-based cytology combined with high-risk HPV genotyping in cervical le-sions%液基细胞学结合HPV高危分型检测对宫颈病变应用价值的评估

    Institute of Scientific and Technical Information of China (English)

    郭周庆; 徐宁; 吴又明

    2014-01-01

    目的:评估宫颈液基细胞学结合高危性HPV DNA检测在宫颈病变中的诊断价值。方法收集本院2009-2012年细胞室及HPV检测室的资料进行评估分析。结果液基细胞学阳性检出率6.01%,HPVDNA高危型分型检出率20.34%,两种方法联合检测的评估指标:阳性检测率33%,敏感性、特异性、阳性预测值、阴性预测值88.58%。结论单独应用上述两种筛查方法都有一定的局限性,但有较高敏感性,阴性预测值及阳性检出率。联合应用检测后不但能够优势互补,而且其敏感性、阴性预测值、阳性检出率更高,但其特异性反而降低。具体原因不详,有待于在今后工作中进一步深入探讨。对已婚妇女在进行细胞学及HPV检测的同时,对HPV检测阳性病例如能采用HPV疫苗治疗,对于HPV检测阴性病例如能早期接种HPV疫苗预防,对于宫颈癌的防治效果会更加科学与完善。%Objective To evaluate the diagnostic value of liquid-based cytologic test combined with high-risk HPV DNA de-tection in the diagnosis of cervical lesions. Methods The data of HPV and liquid-based cytologic test derived from the cell apart-ment and HPV test room in Xiaolan hospital from 2009 to 2012 were collected, evaluated and analyzed. Results The positive de-tection rate of liquid-based cytologic test was 6.01%. The positive rate of high-risk HPV DNA detection was 20.34%. The perfor-mance of the combination method was as follows: the positive detection rate was 33%; the sensitivity was 96.43%; the specificity was 12.62%;the positive predictive rate was 33.49%;the negative predictive rate was 88.58%. Conclusions There exist some cer-tain limitations when using LCT or HPV DNA test along. The two methods shoud be used together. The combination method had a higher sensitivity, negative predictive rate and positive detection rate, but the specificity was lower than that of each single method. HPV vaccine should be

  6. Association of human papillomavirus, Neisseria gonorrhoeae and Chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion

    Science.gov (United States)

    de Abreu, André LP; Malaguti, Natália; Souza, Raquel P; Uchimura, Nelson S; Ferreira, Érika C; Pereira, Monalisa W; Carvalho, Maria DB; Pelloso, Sandra M; Bonini, Marcelo G; Gimenes, Fabrícia; Consolaro, Marcia EL

    2016-01-01

    The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods. A total of 614 (73.3%) of the women had normal cytology (NILM) and 224 (26.7%) women exhibited abnormal cytology (≥ ASC-US). HPV-DNA prevalence was 33.9%, and HPV-16 was the most prevalent genotype in women with NILM and ≥ ASC-US cytology. Non-HPV STDs were detected in 30.4% women and T. vaginalis was the most prevalent one (11.6%). A higher increased risk of ≥ ASC-US and HSIL occurred in co-infections of HR-HPV with C. trachomatis and N. gonorrhoeae. Co-infections of HPV-DNA and HR-HPV with HSV-2 exhibited a similar increased risk but only with ≥ ASC-US. Co-infections of HPV-DNA and HR-HPV with T. vaginalis demonstrated a similar increased risk of ≥ ASC-US and HSIL. We found that C. trachomatis and N. gonorrhoeae were the primary pathogens associated with HR-HPV for the increased risk for all grades of cervical abnormalities but mainly for HSIL, suggesting a possible synergistic action in cervical lesions progression. Our results reinforce the hypothesis that some non-HPV STDs might play a role as co-factors in HPV-mediated cervical carcinogenesis. These data improve our understanding of the etiology of SCC and may also be useful for disease prevention. PMID:27429850

  7. Association of human papillomavirus, Neisseria gonorrhoeae and Chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion.

    Science.gov (United States)

    de Abreu, André Lp; Malaguti, Natália; Souza, Raquel P; Uchimura, Nelson S; Ferreira, Érika C; Pereira, Monalisa W; Carvalho, Maria Db; Pelloso, Sandra M; Bonini, Marcelo G; Gimenes, Fabrícia; Consolaro, Marcia El

    2016-01-01

    The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods. A total of 614 (73.3%) of the women had normal cytology (NILM) and 224 (26.7%) women exhibited abnormal cytology (≥ ASC-US). HPV-DNA prevalence was 33.9%, and HPV-16 was the most prevalent genotype in women with NILM and ≥ ASC-US cytology. Non-HPV STDs were detected in 30.4% women and T. vaginalis was the most prevalent one (11.6%). A higher increased risk of ≥ ASC-US and HSIL occurred in co-infections of HR-HPV with C. trachomatis and N. gonorrhoeae. Co-infections of HPV-DNA and HR-HPV with HSV-2 exhibited a similar increased risk but only with ≥ ASC-US. Co-infections of HPV-DNA and HR-HPV with T. vaginalis demonstrated a similar increased risk of ≥ ASC-US and HSIL. We found that C. trachomatis and N. gonorrhoeae were the primary pathogens associated with HR-HPV for the increased risk for all grades of cervical abnormalities but mainly for HSIL, suggesting a possible synergistic action in cervical lesions progression. Our results reinforce the hypothesis that some non-HPV STDs might play a role as co-factors in HPV-mediated cervical carcinogenesis. These data improve our understanding of the etiology of SCC and may also be useful for disease prevention. PMID:27429850

  8. Bacterial imbalance of the vaginal flora: a cytological and biomolecular concept of Gardnerella vaginalis

    NARCIS (Netherlands)

    Klomp, J.M.

    2008-01-01

    Since 1996 all women aged 30 to 60 years are invited for a cervical smear on a 5 year interval. KOPAC, the Dutch national coding system for cervical smears, introduced for determination of cervical abnormalities also evaluates the inflammatory status of the vaginal flora. In this system dysbacterios

  9. 液基细胞学制片技术与人乳头瘤病毒L1壳蛋白检测对子宫颈病变诊断及进展风险评估%Liquid based cytology test and human papilloma virus L1 Nuclecapsid protein detection in di-agnosis of cervical lesions and its progress

    Institute of Scientific and Technical Information of China (English)

    陈灵芝

    2016-01-01

    Objective:To discuss the clinical value of liquid based cytology test in the diagnosis of cervical lesions and to evaluate the expression and significance of human papilloma virus L1(HPV L1)protein in exfoliative cells of different cervical lesions.Methods:A total of 1400 cases visiting gynaecological clinics of our hospital from January 2009 to August 2013 were se-lected and were given liquid based cytology test (LCT)examination and HPV L1 protein expression detection in exfoliative cells.Meanwhile,cervical biopsy pathology results were taken as baseline for make a comparison.Results:(1)Out of 1400 ca-ses,there were 30 cases with LCT(+),accounting for 2.1%,18 cases with HPV L1 protein detection(+),accounting for 1. 29%;8 cases with LCT and HPV L1 protein detection(+),1360 cases with LCT and HPV L1 protein detection(-).(2) Pathological tissue biopsy was performed on 30 cases of patients with LCT(+),results showed that there were 23 cases with positive result.Pathological tissue biopsy was performed on 18 cases of patients with HPV L1 protein(+),results showed that 14 cases with positive result.Among 1360 cases with LCT and HPV L1 protein detection(-),497 cases accepted the patho-logical biopsy,and the results showed that 1 case presented(+),accounting for 0.2%.Totally 545 cases accepted the patholog-ical biopsy,among whom,38 cases presented(+),accounting for 6.97%;(3)Sensitivity and specificity of LCT combined with HPV L1 protein detection were higher than single-method detection,and difference had statistical significance(P <0.05).Con-clusion:Liquid based cytology test combined with HPV L1 protein detection could improve the diagnostic rate of cervical le-sions,and is expected effective method in the early clinical screening of cervical neoplasia lesions.%目的::探讨液基细胞学制片技术对诊断子宫颈病变的价值,评价人乳头瘤病毒 L1(HPV L1)在子宫颈不同病变脱落细胞中的表达差异及意义.方法:将河

  10. Diagnostic validity of cervical precancerous lesions by liquid-based cytology technique combined with high-risk HPV-DNA detection%液基细胞学技术联合高危HPV-DNA检测对宫颈癌前病变的诊断效度

    Institute of Scientific and Technical Information of China (English)

    王泽曼; 陈玉; 郑建鹏

    2011-01-01

    目的:探讨液基细胞学检查技术(LCT)联合高危人乳头状瘤病毒DNA(HPV-DNA)检测诊断宫颈癌前病变的效度.方法:对2007年12月~2010年12月来我院行体检的19~65岁的6 521名女性采用LCT进行宫颈癌的筛查,以及HPV分型基因芯片检测系统进行18种高危HPV基因亚型检测.对上述检测阳性者行阴道镜下宫颈活组织检查,对检测均阴性者依其意愿进行阴道镜下宫颈活组织检查.结果:LCT阳性(≥ASCUS)152例,HPV阳性86例,其中二者均为阳性的有42例,LCT和HPV均为阴性的有6 325例;LCT阳性的152例和HPV阳性的86例中病理活组织检查结果为阳性(≥CIN I)的分别有112例和68例,其中LCT和HPV均阳性的42例中病理活组织检查阳性的有34例.LCT和HPV均为阴性的6 325例中有2 000人自愿行病理检查,其中1人病理检查结果为阳性.LCT诊断宫颈癌前病变的灵敏度为76.19%,特异度为98.05%;HPV检测诊断宫颈癌前病变的灵敏度为46.26%,特异度为99.12%;两方法联合诊断(其中1项阳性即判定为患者)宫颈癌前病变的灵敏度为99.32%,特异度为99.61%.结论:液基细胞检测技术和高危HPV-DNA检测的联合应用优于单项技术检测,对于宫颈癌前病变的筛检具有重要意义.%AIM: To investigate liquid - based cytology technique ( LCT ) combined with high - risk human papilloma virus DNA ( HPV - DNA ) detection for diagnosis of cervical precancerous lesions. METHODS: Screening of cervical cancer was performed by LCT combined with HPV - DNA detection in 6 521 women at the age of 19 ~65 in our hospital for physical examination from December 2007 to December 2010. Eighteen high - risk HPV isoform genes were detected by HPV typing gene chip detection system. The women with positive detection underwent colposcopic cervical biopsies. The women with negative detection also underwent colposcopic cervical biopsies if the operation was of their own accord. RESULTS: The LCT positive results

  11. DIAGNOSTIC AND THERAPEUTIC POSSIBILITIES IN THE PROPHYLAXIS OF CERVICAL CANCER

    OpenAIRE

    Marzena Wrześniewska; Olga Adamczyk-Gruszka; Jakub Gruszka; Beata Bąk

    2013-01-01

    Poland is one of the countries with high cervical cancer morbidity and mortality. The main means to change this situation is to manage an active and modern programme of cervical cancer prophylaxis and diagnostics. To a large extent, the effectiveness of a cervical cancer prophylaxis programme is decided by the availability of modern diagnostic research. The conventional Papanicolaou test and modern LBC cytology techniques were discussed in the article, taking into consideration HPV diagno...

  12. Apparent rarity of asymptomatic herpes cervicitis in a woman with intra-uterine contraceptive device

    Directory of Open Access Journals (Sweden)

    Adeola Fowotade

    2013-12-01

    Full Text Available Infection with genital herpes simplex virus (HSV remains a common viral sexually transmitted disease, often subclinical and a major worldwide problem of women of reproductive age group. Herpes cervicitis is an unusual presentation of Herpes simplex virus infection in females. The finding of herpes cervicitis on routine pap smear of an asymptomatic woman on Intrauterine contraceptive device still further supports the need for increased awareness on the possibility of Herpes simplex virus infection among women, particularly those on Intrauterine contraceptive device. The index case is a 28 years old Nigerian female who was referred to our Special Treatment Clinic on account of an abnormal pap smear cytology which was in keeping with Herpes cervicitis. There was no history of genital ulcer in this patient; however ELISA for HSV 2 IgM was positive in her. We therefore describe a case of herpes cervicitis in an asymptomatic woman on intrauterine contraceptive device. This case highlights to clinicians the need to be aware of the possibility of this association and to carry out relevant investigations so as to identify and treat these patients appropriately. Therefore, there is a need to put in place adequate public health intervention strategy to prevent genital herpes in women of reproductive age group with a view to preventing the possibility of congenital herpes in subsequent pregnancy.

  13. Raman spectroscopy and oral exfoliative cytology

    Science.gov (United States)

    Sahu, Aditi; Shah, Nupur; Mahimkar, Manoj; Garud, Mandavi; Pagare, Sandeep; Nair, Sudhir; Krishna, C. Murali

    2014-03-01

    Early detection of oral cancers can substantially improve disease-free survival rates. Ex vivo and in vivo Raman spectroscopic (RS) studies on oral cancer have demonstrated the applicability of RS in identifying not only malignant and premalignant conditions but also cancer-field-effects: the earliest events in oral carcinogenesis. RS has also been explored for cervical exfoliated cells analysis. Exfoliated cells are associated with several advantages like non-invasive sampling, higher patient compliance, transportation and analysis at a central facility: obviating need for on-site instrumentation. Thus, oral exfoliative cytology coupled with RS may serve as a useful adjunct for oral cancer screening. In this study, exfoliated cells from healthy controls with and without tobacco habits, premalignant lesions (leukoplakia and tobacco-pouch-keratosis) and their contralateral mucosa were collected using a Cytobrush. Cells were harvested by vortexing and centrifugation at 6000 rpm. The cellular yield was ascertained using Neubauer's chamber. Cell pellets were placed on a CaF2 window and Raman spectra were acquired using a Raman microprobe (40X objective) coupled HE-785 Raman spectrometer. Approximately 7 spectra were recorded from each pellet, following which pellet was smeared onto a glass slide, fixed in 95% ethanol and subjected to Pap staining for cytological diagnosis (gold standard). Preliminary PC-LDA followed by leave-one-out cross validation indicate delineation of cells from healthy and all pathological conditions. A tendency of classification was also seen between cells from contralateral, healthy tobacco and site of premalignant lesions. These results will be validated by cytological findings, which will serve as the basis for building standard models of each condition.

  14. Distribution of high and low risk HPV types by cytological status: a population based study from Italy

    Directory of Open Access Journals (Sweden)

    Pini Maria T

    2011-01-01

    Full Text Available Abstract Background HPV type distribution by cytological status represents useful information to predict the impact of mass vaccination on screening programs. Methods women aged from 25 to 64 who attended cervical cancer screening in five different Italian regions were tested for HPV infection with Hybrid Capture II (HCII low and high risk probes. Women repeating Pap-test upon unsatisfactory or positive results, or as a post-treatment and post-colposcopy follow-up analysis, were excluded from our study. High risk (HR HPV positive samples were typed using GP5+/GP6+ primed PCR, followed by Reverse Line Blot for 18 high/intermediate risk HPV types, while low risk (LR HPV positive samples were tested with type specific primers for HPV6 and HPV11. Results 3410 women had a valid HCII and Pap-test. The prevalence of HR and LR infections was 7.0% and 3.6%, 29.1% and 13.7%, 68.1% and 31.9%, 60.0% and 0.0%, 65.0% and 12.0%, for negative, ASC-US, L-SIL, ASC-H and H-SIL cytology, respectively. The fraction of ASC-US+ cytology due to HPV 16 and 18 ranged from 11.2 (HPV 16/18 alone to 15.4% (including HPV 16/18 in co-infection with other virus strains, and that due to HPV 6 and 11 ranged from 0.2% (HPV 6/11 alone to 0.7% (including HPV 6/11 in co-infection with other LR virus strains. Conclusions mass vaccination with bivalent or quadrivalent HPV vaccine would modestly impact on prevalence of abnormal Pap-test in screening.

  15. Informatics applied to cytology

    Directory of Open Access Journals (Sweden)

    Pantanowitz Liron

    2008-01-01

    Full Text Available Automation and emerging information technologies are being adopted by cytology laboratories to augment Pap test screening and improve diagnostic accuracy. As a result, informatics, the application of computers and information systems to information management, has become essential for the successful operation of the cytopathology laboratory. This review describes how laboratory information management systems can be used to achieve an automated and seamless workflow process. The utilization of software, electronic databases and spreadsheets to perform necessary quality control measures are discussed, as well as a Lean production system and Six Sigma approach, to reduce errors in the cytopathology laboratory.

  16. 桐乡市洲泉镇9000例已婚妇女宫颈癌筛查分析%Analysis of 9 000 cases of married women for cervical cancer screening in Zhouquan town of Tongxiang city

    Institute of Scientific and Technical Information of China (English)

    范利梅; 俞翠芳

    2015-01-01

    目的:了解桐乡市洲泉镇所辖地区已婚妇女宫颈上皮内瘤样病变(CIN)的患病情况,为制定干预措施提供依据。方法:调查桐乡市洲泉镇经液基细胞学检查的已婚妇女9000例,采用TBS细胞学诊断标准;并对液基细胞学检查有异常者行阴道镜下宫颈活组织检查。结果:液基细胞学检查异常301例;阴道镜下宫颈活组织检查261例,诊断CIN 68例(26.15%),占液基细胞学结果异常者22.59%,其中CIN Ⅰ38例,CIN Ⅱ18例,CIN Ⅲ12例;原位癌1例,早期浸润癌0。结论:桐乡市洲泉镇所辖已婚妇女CIN的患病率0.76%,早期对CIN干预和治疗可有效降低宫颈癌的发生率。%Objective:To understand married women for cervical cancer screening in Zhouquan town of Tongxiang city,to provide the basis for the intervention measures.Methods:Investigating 9 000 cases of liquid based cytology for married women of in Zhouquan town of Tongxiang city,using standard cytological diagnosis of TBS,and liquid-based cytology abnormalities underwent colposcopy cervical biopsy.Results:301 cases of abnormal liquid based cytology,colposcopic cervical biopsy tissue was examined in 261 cases,the diagnosis of CIN in 68 cases(26.15%),accounting for liquid based cytology results abnormal 22.59%,of which 38 cases of CIN Ⅰ,CIN Ⅱ in 18 cases,CIN Ⅲ in 12 cases,orthotopic carcinoma in 1 cases and early invasive carcinoma in 0 cases. Conclusion:Zhouquan town of Tongxiang city of the prevalence of CIN in married women was 0.76% .Early intervention and treatment for CIN can effectively reduce the incidence of cervical cancer.

  17. Clinical evaluation of cytological diagnosis of nasopharyngeal malignancies.

    Science.gov (United States)

    Molinari, R; Pilotti, S; Rilke, F

    1978-01-01

    Between 1970 and 1975 cytological examination was applied to the diagnosis of nasopharyngeal malignancies in a series of 216 consecutive patients who had either a tumour in the nasopharynx or clinical signs of nasopharyngeal carcinoma, or who were locally asymptomatic but had enlarged cervical lymph nodes. Smears were taken by introducing a small rough pad of compressed gauze through the mouth into the nasopharynx with an upward-angled forceps. In each case the cytological smear was taken immediately before biopsy; often, a lymph node was removed subsequently. When morphological diagnoses were doubtful and histological findings were at variance with positive cytological findings, the patients were reexamined clinically, and diagnosis was postponed. The case material was made up of 90 nasopharyngeal carcinomas, 24 lymphomas, one malignant melanoma, one adenoid cystic carcinoma and 100 patients without malignancies. Cytological findings from the first smear were positive in 77.8% of nasopharyngeal carcinomas, in 66.6% of lymphomas and in the cases of melanoma and adenoid cystic carcinoma. There were no false-positive results. When the nasopharyngeal carcinomas were subdivided into undifferentiated carcinomas of the nasopharyngeal type and squamous-cell carcinomas, cytological findings were positive in ,0% and 73%, respectively. Positivity of histological findings was distributed as follows: 91.7% for malignant lymphomas, 86.6% for undifferentiated carcinomas and 86.6% for squamous-cell carcinomas. With respect to clinical suspicion of malignancy, positive cytological findings were obtained in 50% of clinically occult cases and in 84.6% of patients with obvious malignancies; intermediate figures were found for clinically doubtful (64.3%) and for highly suspicious (77.8%) cases. Cyto-histological concordance was shown in 70% of cases; false-negative histological results were obtained in 7.8% and false-negative cytological results in 16.6% of cases. Combined cyto

  18. 叶酸受体新技术及细胞学检查对宫颈癌筛查应用价值的评估%Evaluation of the application of folate receptor mediated (FRD) multifunctional acetic acid white solution inspection with liquid based cytology screening for cervical cancer

    Institute of Scientific and Technical Information of China (English)

    邓翠; 康佳丽; 蒋文燕; 聂妙玲; 黄晓晖

    2014-01-01

    Objective To explore the folate receptor mediated (FRD) check and multi-function acetic acid white solution liquid based cervical cytology (TCT) application value in cervical cancer screening.Methods A total of 602 cases of patients was tested with FRD multi-function acetic acid white solution check,and TCT and cervical biopsy pathology examination.With the used of histopathological results as the gold standard,FRD multi-function acetic acid white analysis was compared with the TCT screening inspection results.Results For a total 602 patients with TCT screening,the positive rate was 21.8% (131/602),including 36 cases of CIN Ⅰ level,41 cases of CIN Ⅱ level,24 cases of CIN Ⅲ level,and 30 cases of cervical invasive carcinoma.For the FRD multifunction white acetate solution screening,its positive rate was 23.8% (143/602).No statistically significant difference was found between TCT and FRD screening (P > 0.05).The missed diagnosis rate of FRD multi-function white acetate solution screening was 2.6% in inflammation,and 21.1% in cervical invasive cancer,and 3.8 % in CIN.The missed diagnosis rate of TCT screening was 7.2% in inflammation,5.3% in CIN Ⅰ,4.9% in CIN Ⅱ,and 58.6% in CIN Ⅲ]; whereas,its detection coincidence rate was 100% in squamous cells carcinoma (SCC) and adenocarcinoma (AC).FRD multi-function acetic acid white solution screening had a sensitivity 80.92%,specificity 92.14%,positive predictive value 74.13%,and negative predictive value 95.59%.TCT examination had a sensitivity 90.84%,specificity 90.23%,positive predictive value 72.12%,and negative predictive value 97.25%.No significant difference was found between FRD and TCT methods (P > 0.05).Conclusions FRD and TCT methods were both efficient in screening and evaluation for cervical lesions and cervical cancer.Because FRD method is limited in the deep tube for examination of cervical lesions; it cannot completely replace the TCT examination

  19. Cytologic features of pulmonary blastoma

    Directory of Open Access Journals (Sweden)

    Sai Shalini C

    2009-01-01

    Full Text Available Pulmonary blastomas are rare lung neoplasms constituting 0.5% of all lung tumors. This tumor has an aggressive course and needs to be recognized on cytology. A preoperative diagnosis of pulmonary blastoma is difficult to obtain by cytopathologic methods. A diagnosis of biphasic pulmonary blastoma should be considered when there is a dimorphic population of cells on cytology. A 30-year-old male presented with gradually progressing breathlessness and left-sided chest pain for the past one month. Chest radiograph and computed tomography of thorax revealed an anterior mediastinal mass that was subjected to ultrasound-guided fine-needle aspiration cytology. Aspiration cytology showed a highly cellular lesion with a dimorphic population of tumor cells in a necrotic background. The possibility of a non-small cell carcinoma was suggested. Subsequent histopathology revealed the tumor to be a pulmonary blastoma. The importance of recognizing the dimorphic population of cells in cytology is discussed.

  20. Are all left supraclavicular lymphadenopathies Virchow's? A retrospective study of 320 left cervical lymphadenopathy

    OpenAIRE

    Veena Raja; Chinnaiya Subramaniam; Babu Rajendra Prasad; Mari Lingiah Harendra Kumar; Balluru Vasudeva Swaroop Raj

    2016-01-01

    Background: Cervical Lymphadenopathy is one of the commonest and sometimes only clinical finding for a benign and malignant lesion. Fine needle aspiration cytology (FNAC) is a simple, safe, rapid and inexpensive method for establishing the diagnosis of lesions. Metastasis is more common in cervical lymph node and enlarged cervical nodes in an elderly patient must be considered as metastatic until proved otherwise. Methods: Retrospectively, 320 cases of left cervical lymph node FNAC were colle...

  1. Detection, management, and follow-up of pre-malignant cervical lesions and the role for human papillomavirus.

    NARCIS (Netherlands)

    Hamont, D. van; Bekkers, R.L.M.; Massuger, L.F.A.G.; Melchers, W.J.G.

    2008-01-01

    Cervical cytological pathology is common. Prevention of cervical cancer by detecting the disease process at an early and pre-malignant stage is practised globally either through population-based screening programmes (PSP) or through non-organised ones. High-grade cervical intraepithelial neoplasia (

  2. Analysis on the results of cervical disease liquid-based cytology and human papillomavirus detection in 708 migrant female workers in Fengxian District, Shanghai%上海奉贤地区708例外来务工妇女宫颈疾病液基细胞学及人乳头瘤病毒检测结果分析

    Institute of Scientific and Technical Information of China (English)

    顾其龙; 凤敏华

    2013-01-01

    Objective To investigate the prevalence of cervical diseases and high-risk human papillomavirus (HR-HPV) infection in migrant female workers in Fengxian District, Shanghai. Methods The results of liquid-based cytology and HR-HPV detection of 708 migrant female workers seeking medical advice for possible cervical diseases in Fengxian District, Shanghai were analyzed retrospectively. The results of biopsy and loop electrosurgical excisional procedure ( LEEP) from a part of these patients were also analyzed. Results Among these migrant female workers,the prevalences of atypical squamous cell ( ASC ) , low-grade squamous intraepithelial lesion ( LSIL) and high-grade squamous intraepithelial lesion (HSIL) were 31. 6% ,23. 2% and 11.9% Respectively. The prevalence of HR-HPV was 68.4%. With the increase of age, the prevalences of LSIL and HR-HPV decreased, but the prevalence of HSIL increased. Cervical intraepithelial neoplasia or worse ( CIN2 + ) results were found by biopsy in 35. 7% of HR-HPV positive ASC patients and 37.8% of HR-HPV positive LSIL patients,respectively. Among HSIL patients,85. 7% were CIN2 + by biopsy or LEEP. Conclusions The prevalence of cervical diseases is high among migrant female workers in Fengxian District,Shanghai. Some of them have high-grade lesions.%目的 研究上海奉贤地区外来务工妇女宫颈疾病发生及高危型人乳头瘤(HR-HPV)感染情况.方法 回顾性分析上海奉贤区708例因疑似宫颈疾病就诊的外来务工妇女液基细胞学和HR-HPV检测结果,以及部分患者的活检病理结果和宫颈环形电切(LEEP)病理结果.结果 上述患者中非典型鳞状细胞(ASC)、低度鳞状上皮病变(LSIL)和高度鳞状上皮内病变(HSIL)的发生率分别为31.6%、23.2%和11.9%,HR-HPV的感染率为68.4%.随着年龄的增长,LSIL发生率显著降低,HR-HPV感染率逐渐降低,而HSIL发生率则逐渐升高.HR-HPV阳性的ASC和LSIL患者中活检病理结果

  3. Human papillomavirus and infections of the lower genital tract in women with abnormal cervical cytological examination = Papilomavírus humano e infecções do trato genital inferior em mulheres com exame colpocitológico anormal

    Directory of Open Access Journals (Sweden)

    Paesi, Suelen

    2013-01-01

    Conclusões: Não houve associação da infecção pelo HPV com outras infecções do trato genital feminino nem com características sociodemográficas. Múltiplas infecções com até cinco tipos de HPV foram observadas em uma pequena parcela das mulheres. É difícil definir claramente um grupo de risco para a infecção por HPV. Todas as mulheres com exame colpocitológico anormal são possíveis portadoras do HPV e de outras infecções do trato genital inferior

  4. 液基细胞学联合 HPV 分型检测在妊娠合并宫颈上皮内瘤变患者的应用%Liquid-based cytology in combination with human papilloma virus test for cervical intraepithelial neoplasia during pregnancy

    Institute of Scientific and Technical Information of China (English)

    郑寰宇; 马冬; 李鸥; 李月红; 张丽芬; 刘桂艳; 杨利华

    2013-01-01

    Objective: The present study aimed to investigate the relationship between cervical intraepithelial neoplasia (CIN) and human papilloma virus (HPV) infection during pregnancy using liquid-based cytology and HPV test. Methods: We prospectively studied the clinical data of 72 pregnant women with CIN who were hospitalized between January 2006 and January 2012 at Tangshan Gongren Hospital. All patients underwent liquid-based thin-layer cytology technique and/or HPV test. Results: Thirty two cases, which included 16 cases each of CIN Ⅰ and Ⅱ, displayed high-grade squamous intraepithelial lesion. The other 40 cases were CIN Ⅲcharacterized by low-grade squamous intraepithelial lesion based on biopsy pathology through colposcopy. A total of 48 (85.7% ) HPV-positive cases were determined among 56 antenatal patients. Moreover, 32 of the 48 HPV-positive cases had either HPV16 infection alone or a mixture of different infection types. Conclusion: Results indicated that the CIN pregnant patients exhibited high frequency of HPV infections, and its HPV16 type is closely associated with the pathological development of high-grade lesions.%  目的:应用液基细胞学联合HPV分型方法检测妊娠合并宫颈上皮内瘤变(Cervical intraepithelial neoplasia,CIN),初步了解此类患者中TCT及HPV感染情况与妊娠宫颈病变的关系.方法:对象为2006年1月至2012年1月确诊为妊娠合并宫颈上皮内瘤变的72例患者,初次孕期保健均进行细胞学、HPV检测,对这些临床资料进行回顾性分析研究.结果:妊娠合并CIN发生率为2.1%.72例患者中全部产前行宫颈TCT检测,HSIL为32例,LSIL为40例,56例于产前保健时检测HPV,其中48例(85.7%)为阳性结果,32例为HPV16型单独或混合感染.72例孕期保健时行阴道镜活检病理诊断CINⅠ16例、CINⅡ16例、CINⅢ40例.结论:液基细胞学联合HPV分型检测在妊娠合并宫颈上皮内瘤变患者的应用是安全而且有效的.在妊娠

  5. 分析改良阴道取样巴氏涂片及液基薄层细胞学检查对宫颈癌的诊断价值%Analysis the diagnostic value of improved vaginal sampling Pap smear and thinprep cytology for cervical cancer

    Institute of Scientific and Technical Information of China (English)

    韩胜春

    2015-01-01

    Objective Analysis of the value of improved vaginal sampling Pap smear and liquid based cytologyexamination in diagnosis of cervical cancer.Methods In our hospital for health examination of 683 cases of female, with improved vaginal sampling Pap smear, and ThinPrep cytological test (TCT), TCT for the detection of positive patients who underwent colposcopic multiple biopsy in pathological diagnosis as the criterion.Summary of analysis of test Results .Results In 683 specimens, there are unsatisfactory in 12 cases, specimensof satisfaction is 98.2% (671/683). In 671 specimens including 52 cases of Trichomonas, mold in 17 cases, 42cases of clue cells. TCT detection of squamous cell carcinoma antigen (SCC)、coincidence detection and biopsy of AC and rate of HSIL is higher than LSIL, the difference was Statistically signiifcant (P<0.05).Conclusion The improved vaginal sampling Pap smear and liquid based cytology examination on the positive detection rate of squamous cell carcinoma, adenocarcinoma and high-grade squamous intraepithelial lesion is higher, and the method of economic security, convenient operation, suitable for cervical cancer screening work of the majority of the masses of women.%目的 分析改良阴道取样巴氏涂片及液基薄层细胞学检查对宫颈癌的诊断价值.方法 选取在我院进行健康体检的女性683名,采用改良阴道取样巴氏涂片,并进行液基薄层细胞学检查(TCT),对于TCT检测阳性患者行行阴道镜下宫颈多点活检,以病理诊断为判定标准.总结分析检测结果.结果 683例标本中,有不满意标本12例,标本满意度为98.2%(671/683).671例标本中,滴虫52例,霉菌17例,线索细胞42例.TCT检测对鳞状上皮细胞癌抗原(SCC)、腺癌(AC)及鳞状上皮内高度病变(HSIL)的检测与病理活检的符合率高于LSIL,差异均有统计学意义(P<0.05).结论 改良阴道取样巴氏涂片及液基薄层细胞学检查对鳞癌、腺癌及鳞状上皮内高度病变有

  6. 细针穿刺细胞学及细胞块技术在颈部肿大淋巴结诊断的意义%Diagnostic Significance of the Fine Needle Aspiration Cytology and Cell Block Technology in Cervical Lymphadenopathy

    Institute of Scientific and Technical Information of China (English)

    张春梁; 陈水平; 黄丽云; 晋雯

    2014-01-01

    Objective To explore the diagnostic significance of combined use of the fine needle as-piration cytology (FNAC) and cell block technique (FNACB) in the cervical lymphadenopathy . Methods 109 cases of cervical lymphadenopathy were examined by combined FNAC and FNACB as well as immu-nocytochemistry (ICC) when necessary ,and compared with FNAC only to analyze the diagnostic accuracy . Results The diagnostic accuracy of FNAC ,FNAC + FNACB ,and FNAC + FNACB + ICC was 51 .4% ,78 .9% and 95 .4% ,respectively . Conclusion FNAC combined with FNACB and ICC is of higher diagnostic value but has some limitations in accurate diagnosis of poorly differentiated carcinomas and malignant lymphoma .%目的:探讨细针穿刺细胞学联合细胞块技术对于明确诊断颈部肿大淋巴结的意义。方法选择颈部肿大淋巴结病例109例行细针穿刺涂片并制备细胞块切片,必要时行免疫细胞化学染色检测,比较单纯常规穿刺涂片与涂片联合细胞块切片检查结果,分析相应技术诊断的准确性。结果109例中,单纯常规细针穿刺涂片与最终诊断的符合率仅为54.1%,联合细胞块切片符合率为78.9%,免疫细胞化学染色检查后符合率则达到95.4%。结论常规细针穿刺涂片联合细胞块切片及免疫细胞化学染色对于颈部肿大淋巴结诊断有较高的应用价值,但对于少数差分化肿瘤及恶性淋巴瘤的诊断存在一定局限性。

  7. Malignant atypical cell in urine cytology: a diagnostic dilemma

    Directory of Open Access Journals (Sweden)

    Kakkar Nandita

    2006-01-01

    Full Text Available Abstract Aims The aim of this study was to find out the characteristic morphology of malignant atypical cells which were missed on routine cytology of urine. Materials and methods In this retrospective study, we examined detailed cytomorphology of 18 cases of atypical urinary cytology which were missed on routine examination and were further proved on histopathology as transitional cell carcinoma (TCC of bladder. The cytological features of these cases were compared with 10 cases of benign urine samples. Results There were 11 cases of high grade TCC and 7 cases of low grade TCC on histopathology of the atypical urine samples. Necrosis in the background and necrosed papillae were mostly seen in malignant atypical cells. The comet cells and cells with India ink nuclei (single cells with deep black structure-less nuclei were only observed in malignant atypical cells. The most consistent features in malignant atypical cells were: i high nuclear and cytoplasmic (N/C ratio ii nuclear pleomorphism iii nuclear margin irregularity iv hyperchromasia and v chromatin abnormalities Conclusion The present study emphasizes that nuclear features such as high N/C ratio, hyperchromasia and chromatin abnormalities are particularly useful for assessing the malignant atypical cells. Other cytological features such as comet cells and cells with India ink nuclei are also helpful for diagnosis but have limited value because they are less frequently seen.

  8. Fatores de risco para câncer de colo do útero segundo resultados de IVA, citologia e cervicografia Factores de riesgo para cáncer de cuello uterino según resultados de IVA, citología y cervicografía Risk factors for uterine cervical cancer according to results of VIA, cytology and cervicography

    Directory of Open Access Journals (Sweden)

    Saiwori de Jesus Silva Bezerra dos Anjos

    2010-12-01

    ,001. No se encontró asociación significativa en la cervicografía.This study aimed to evaluate the association between risk factors for uterine cervical neoplasms and cervical lesions by HPV by comparison of the visual inspection with acetic acid (VIA, cytology and cervicography results. A prevalence research was made with 157 women in a health center of Fortaleza in the period of June to September 2006. The SPSS program was used to codify the data. Inferences were made through statistical tests (χ2= chi square and LR= likelihood ratio. The VIA, cervicography and cytology obtained 43.3%, 10.19% and 3.2% of altered results. The variables with important association to cervical lesions in the VIA were: aged less than 20 years old (p= 0.0001; one or more partners in the last three months (p= 0.015; use of contraceptives (p = 0.0008; presence of vaginal discharge (p= 0.0001 and moderate or accentuated inflammatory process (p= 0.0001. In the cytology: low instructional level (p= 0.0001 and high pH (p= 0.001. It wasn't found any significant association in the cervicography.

  9. Cervical cancer: screening, diagnosis and staging.

    Science.gov (United States)

    Tsikouras, Panagiotis; Zervoudis, Stefanos; Manav, Bachar; Tomara, Eirini; Iatrakis, George; Romanidis, Constantinos; Bothou, Anastasia; Galazios, George

    2016-01-01

    Purpose: Despite the widespread screening programs, cervical cancer remains the third most common cancer in developing countries. Based on the implementation of cervical screening programs with the referred adoption of improved screening methods in cervical cytology with the knowledge of the important role of the human papilloma virus (HPV) it's incidence is decreased in the developed world. Even if cervical HPV infection is incredibly common, cervical cancer is relatively rare. Depending on the rarity of invasive disease and the improvement of detection of pre-cancerous lesions due to the participation in screening programs, the goal of screening is to detect the cervical lesions early in order to be treated before cancer is developed. In populations with many preventive screening programs, a decrease in cervical cancer mortality of 50-75% is mentioned over the past 50 years. The preventive examination of vagina and cervix smear, Pap test, and the HPV DNA test are remarkable diagnostic tools according to the American Cancer Association guidelines, in the investigation of asymptomatic women and in the follow up of women after the treatment of pre-invasive cervical cancer. The treatment of cervical cancer is based on the FIGO 2009 cervical cancer staging.

  10. Extraction of DNA from exfoliative cytology specimens and its suitability for analysis by the polymerase chain reaction.

    Science.gov (United States)

    Jackson, D P; Payne, J; Bell, S; Lewis, F A; Taylor, G R; Peel, K R; Sutton, J; Quirke, P

    1990-01-01

    The extraction of DNA from archival exfoliative cytology samples would allow the molecular biological analysis of this readily available material using the polymerase chain reaction (PCR). We have quantitatively and qualitatively studied the extraction of DNA from a variety of cytological preparations. For both fresh and archival cervical smears, overnight incubation with proteinase K produces high yields of high molecular weight DNA, but simply boiling the samples produces DNA suitable for PCR amplification of a single copy gene. Increasing the proteinase K incubation to several days allows the extraction of DNA from fixed and stained archival cytology slides from a variety of sites. The extracted DNA was again suitable for PCR analysis. Fresh and archival cytological material can be utilized for molecular biological study of disease processes using PCR. Archival cytological material is probably the best source of DNA and RNA after stored frozen tissue.

  11. [Cytological finding in the pre- and early stages of cervix carcinoma--a contribution to the evaluation of Papanicolau III].

    Science.gov (United States)

    Bader, G; Büttner, H H; Neumann, H G; Rhode, E; Beust, M

    1977-01-01

    Cytologic findings and the histologic diagnosis are compared in 326 cervical cones. We have found following ratio of the groups Papanicolaou (Pap) III: Pap IV--in dysplasia 1: 1: 1, in "more dysplasia than carcinoma in situ (CIS)" 1:2:2. The Pap IV dominates in "pure" CIS and in cones with "more CIS than dysplasia". We take out of the Pap III ("with cytologic control") cases named "Pap III with necessity for histologic diagnosis". We have found in this subgroup of Pap III prestages or early stages of cervical carcinoma.

  12. Abnormal apoptosis and human papilloma virus infection in carcinogenesis of cervical carcinoma%凋亡异常与人乳头瘤病毒感染在宫颈癌发生中的意义

    Institute of Scientific and Technical Information of China (English)

    李素红; 刘玲玲; 连婧; 王全红; 白玮

    2012-01-01

    Objective To investigate the impact of abnormal human telomerase activity and high-risk human papilloma virus (HPV) infection on the carcinogenesis of cervical carcinoma, and to analyze their correlation with abnormal expression of cyclin. Methods The expressions of human telomerase gene (hTERC), high-risk HPV DNA 16/ 18, 31/33 and cyclins (P16ink4a, CDK4, Rb, Ki67) in squamous cell carcinoma and cervical intraepithelial neoplasia (CIN) were detected using fluorescence in situ hybridization, chromogenic in situ hybridization and immunohistochem-ical assay, as were normal cervical tissues designed as control. Results There was a marked increase in the amplification of hTERC gene, expression of high-risk HPV, Rb, P16ink4a, CDK4 and Ki67 in squamous cell carcinoma and CIN as compared with normal controls. Infection of moderate-to-high-risk HPV in squamous cell carcinoma was positively correlated with hTERC gene expression. A significantly higher amplification of hTERC was presented in advanced squamous cell carcinoma than that in low-grade carcinoma. Rb was correlated with the positive expression of Ki-67. There was positive correlation between the positive expression of CDK4 and histopathologic grading of squamous cell carcinoma. Also revealed was the correlation between HPV16/18 infection and intravascular tumor embolus. Conclusion Development of cervical carcinoma is closely correlated with high-risk HPV infection. Synergistic actions from infection with high-risk HPV and the resulting hTERC gene abnormal amplification may lead to carcinogenesis. The amplification of hTERC gene may be used as a predictor for progression of cervical precancerous lesion, and P16ink4a, CDK4 and Ki67 may be used to indicate the severity of cervical precancerous lesion in adjunctive diagnosis.%目的 探讨宫颈鳞癌发生过程中端粒酶活性异常和高危型人乳头瘤病毒(HPV)感染的意义,同时分析与细胞周期蛋白异常表达的关系.方法 应用荧光原位

  13. False-positive Human Papillomavirus DNA tests in cervical screening

    DEFF Research Database (Denmark)

    Rebolj, Matejka; Pribac, Igor; Lynge, Elsebeth

    2011-01-01

    Based on data from randomised controlled trials (RCT) on primary cervical screening, it has been reported that the problem of more frequent false-positive tests in Human Papillomavirus (HPV) DNA screening compared to cytology could be overcome. However, these reports predominantly operated with a...... narrow definition of a (false-)positive test. The aim of this paper was to illustrate how the narrow definition affected the measured adverse effects of HPV DNA screening compared with cytology screening....

  14. Evaluation of novel assays for the detection of human papilloma virus in self-collected samples for cervical cancer screening.

    Science.gov (United States)

    Chen, Q; Du, H; Zhang, R; Zhao, J H; Hu, Q C; Wang, C; Wang, G X; Tang, J L; Wu, R F

    2016-01-01

    The aim of this study was to evaluate the performance of three new high-risk human papillomavirus (HPV) assays for primary cervical cancer screening, by using self-collected samples, and to identify an HPV assay that could overcome the major obstacles faced during large-scale population-based screening. Two hundred and ten women showing abnormal cervical cytology (and referred for a colposcopy) were recruited in this study. Self-collected samples obtained from all women were tested with the Cobas, Seq, and BioPerfectus Multiplex Real Time HPV assays; simultaneously, clinician-collected samples (from the same women) were tested with the gold-standard Cobas HPV assay. The results of all the assays were consistent. The sensitivity, positive predictive value, and negative predictive value for cervical intraepithelial neoplasia 2+ (CIN2+) and CIN3+ were comparable between the self-collected samples tested with the three new assays and the clinician-collected samples tested with the Cobas HPV assay (P > 0.05). The single-genotype HPV load per sample did not differ significantly between the self- and clinician-collected samples (P = 0.195). In conclusion, the results of this study demonstrated the applicability of the three new HPV assays for primary cervical cancer screening based on self-collection. PMID:27420961

  15. DNA定量分析联合阴道镜在诊断宫颈癌及高级别宫颈上皮内瘤变中的价值%Value of DNA quantitative analysis combined with colposcopy for diagnosis of cervical cancer and high- grade cervical intraepithelial neoplasia

    Institute of Scientific and Technical Information of China (English)

    李苗; 刘芳芳; 邸媛媛; 邓清华; 钟培根

    2012-01-01

    目的:探讨宫颈细胞DNA定量分析法联合阴道镜在宫颈癌及高级别宫颈上皮内瘤变(CIN)诊断中的价值.方法:对4 577名妇女的宫颈细胞采用DNA定量分析系统和常规细胞学进行检查,常规细胞学诊断低级别鳞状上皮内瘤变(LSIL)及以上级别或DNA定量分析异常者在阴道镜下行宫颈活组织病理检查.结果:50名妇女做了病理活检,病理诊断宫颈癌及CIN 2以上病变共27例,其中DNA定量分析方法异常25例(92.6%),常规细胞学异常6例(22.2%),阴道镜异常22例(81.5%),三者间差异有统计学意义(P<0.005).DNA定量分析及阴道镜检查筛查子宫颈癌及高级别宫颈上皮内瘤变(≥CIN2)的敏感度、特异度均高于常规细胞学.结论:DNA定量分析方法联合阴道镜在早期诊断宫颈癌及高级别宫颈上皮内瘤变方面有更高的敏感性,适合在基层单位大力推广.%Objective: To explore the value of DNA quantitative analysis of cervical cells combined with colposcopy for diagnosis of cervical cancer and high-grade cervical intraepithelial neoplasia (CIN) . Methods: The cervical cells of 4 577 women were examined by DNA quantitative analysis system and routine cytologic test, the women diagnosed as low - grade squamous intraepithelial lesion (LSIL) and lesions above by routine cytologic test and the women with abnormal results of DNA quantitative analysis underwent cervical biopsy under col-poscope and pathological examination. Results: Fifty women underwent pathological biopsy, and 27 women were diagnosed as cervical cancer and &CIN Ⅱ lesions, 25 women (92. 6% ) were found with abnormal results of DNA quantitative analysis, 6 women (22. 2% ) were found with abnormal results of routine cytologic test, 22 women (81. 5% ) were found with abnormal results of colposcopy, there was statistically significant difference among the three rates (P < 0. 005 ) . The sensitivities and specificities of DNA quantitative analysis and

  16. 电针深刺颈夹脊穴治疗单纯性颈椎曲度异常临床随机对照观察%Clinical Randomized Control Observation on the Treatment of Cervical Curvature Abnormality by Deep Electro-acupuncture at Cervical Jiaji Points

    Institute of Scientific and Technical Information of China (English)

    吴曦; 段超; 彭锐

    2015-01-01

    Objective:To observe the effect of deep electro -acupuncture at cervical Jiaji points on simple cer-vical curvature abnormality and its clinical value.Methods:138 patients with simple cervical curvature abnor-mality ( from the loss of follow-up of the Group actually 131 ) in random questionnaires were randomly divided into three groups,electro -acupuncture ( EA ) deep needling Group ( group A ) with 53 cases; common EA Group (Group B)with 46 cases;the physical therapy group (Group C)with 32 cases.Group A:C3 ~C6 spinous level deep sting treated with EA at Jiaji points,with tip touching bone;Group B received levels of C 3~6 spinous Jiaji points EA,but the tip did not touch the bone;Group C:cervical physical therapy such as electrotherapy and ultra short wave radiation.All three groups were treated for 10 days.Three groups before treatment and six months after treatment were conducted by X -ray examination.Measure cervical curvature by the Borden's meas-urement for statistical analysis.Results:Measured values in Groups A,B and C before treatment were (6.9 ± 1.9)mm,(7.3 ±2.1)mm and(6.9 ±1.7)mm;Six months after treatment measurements in Groups A,B and C were(14.1 ±2.6)mm,(10.1 ±1.5)mm and(9.7 ±1.9)mm.The levels of recovery of cervical curvature after treatment between group A and group B or group C had a statistically significant difference ( P<0.01 ).Conclusion:EA deep acupuncture at cervical Jiaji points for the treatment of simple cervical curvature abnor-mality is effective,and is superior to the common EA and physical therapy.%目的:观察电针深刺颈夹脊穴疗法对单纯性颈椎曲度异常的影响并判断其临床价值.方法:138名单纯性颈椎曲度异常患者(因失随访入组实际131人)按随机量表法(分段随机化)分为3组,电针深刺组(A组)53人、普通电针组(B组)46人、物理治疗组(C组)32人.A组接受C3 ~C6 棘突水平夹脊穴深刺电针治疗,针尖触及骨质;B组接受C3 ~C6 棘突水平夹脊穴电针治

  17. Detection of telomerase, its components, and human papillomavirus in cervical scrapings as a tool for triage in women with cervical dysplasia

    NARCIS (Netherlands)

    Reesink-Peters, N.; Helder, M N; Wisman, G B A; Knol, A J; Koopmans, S; Boezen, H M; Schuuring, E; Hollema, H; de Vries, Elisabeth G. E.; de Jong, Steven; van der Zee, A G J

    2003-01-01

    AIM: To examine whether the detection of either telomerase and its components or high risk human papillomavirus (HPV) are of value in predicting the presence of cervical intraepithelial neoplasia (CIN) grade II/III in women referred because of cervical cytology reports showing at most moderate dyska

  18. ANTERIOR OSTEOPHYTE IDENTIFICATION IN CERVICAL VERTEBRAE

    Directory of Open Access Journals (Sweden)

    A. T. Chougale

    2011-06-01

    Full Text Available Radiologist always examines X-ray to determine abnormal changes in cervical, lumbar & thoracic vertebrae. Osteophyte (bony growth may appear at the corners of vertebrae so that vertebral shape becomes abnormal. This paper presents the idea from Image processing techniques such as customised Hough transform which will be used for segmentation which should be independent of rotation, scale, noise & shape. This segmented image will be then used for computing size invariant, convex hull based features to differentiate normal cervical vertebrae from cervical vertebrae containing anterior osteophyte. This approach effectively finds anterior osteophytes in cervical vertebrae.

  19. Testicular cytology in azoospermia

    Directory of Open Access Journals (Sweden)

    Usha Prasad

    2015-06-01

    Results: The commonest group with infertility were in the age group 21-30 years. The testicular size was normal in 87.17% of subjects and 12.82% had small testis. Out of 39 subjects with azoospermia 38.46% subjects had varicocele. Varicocele was commonly associated with duct obstruction. The commonest causes observed in cases with azoospermia were; hypospermatogenesis with maturation arrest (4/39,10.25%, duct obstruction (12/39,30.76%, maturation arrest (7/39,17.94%, testicular atrophy (10/39,25.64% and sertoli cell only syndrome (2/39,5.12%. In the present study diagnostic accuracy was 89.18%. Conclusions: Fine needle aspiration cytology is as informative as biopsy and can be done as a routine procedure. It helps us in ruling out obstructive and non-obstructive causes for azoospermia. In cases where FNAC shows normal spermatogenesis with azoospermia, biopsy and Doppler study is indicated to rule out duct obstruction which can be corrected surgically. It is a simple and cost effective. [Int J Reprod Contracept Obstet Gynecol 2015; 4(3.000: 647-652

  20. [Cervical cancer screening in Switzerland - current practice and future challenges].

    Science.gov (United States)

    Untiet, Sarah; Schmidt, Nicole; Low, Nicola; Petignat, Patrick

    2013-04-01

    At the beginning of the 20th Century, cervical cancer was the leading cause of death from cancer in women. A marked decline in cervical cancer has been observed since the 1960s, in parallel with the introduction of the Papanicolau (Pap) test as a cytological screening method. Today, Pap smear screening is still the most widely used tool for cervical cancer prevention. Testing for human papillomavirus (HPV) in cervical specimens or a combination of Pap and HPV testing are also now available. In this article we compare current guidelines for cervical cancer screening in Switzerland with those in other European countries. In view of the opportunities offered by HPV testing and, since 2008, HPV vaccination, current guidelines for cervical cancer screening should be updated. Both the choice of screening tests and general organization of cervical cancer screening should be reviewed.

  1. Diagnosis of cervical cancer with transvaginal color Doppler sonography

    Directory of Open Access Journals (Sweden)

    Li-bo DENG

    2011-09-01

    Full Text Available Objective To investigate the imaging features of cervical cancer by transvaginal color Doppler sonography(TVCS,and evaluate the diagnostic value of TVCS.Methods A hundred and thirty cases of cervical intraepithelial neoplasia(CIN grade Ⅰ-Ⅱ and cervical cancer,diagnosed by Thinprep cytologic test(TCT,cervical biopsy and pathological examination,received TVCS examination.The image characters and color Doppler flow imaging(CDFI were collected and analyzed.Another 41 cases with normal cervices as determined by inspection and cytological examination were involved as control.Results In order of normal cervix,CIN,cancer in situ and cervical cancer,the cervical diameter showed a tendency of increase,also with an increase incidence of low-level echo focus in cervix.As a specific image of cervical cancer,the low level echo focus occurred only in cervical cancer with a specificity of 100%.The absence of mucosal line in cervical canal was a specific character of stage Ⅱ cervical cancer with a specificity of 100%.CDFI and resistance index(RI revealed that the local blood flow was more abundant in invasive cancer than in CIN and cancer in situ,and significant difference was found between stage Ⅰ and stage Ⅱ cervical cancer(P < 0.05.The sensitivity and specificity of enlarged cervical diameters in diagnosis of cervical cancer were 89.1% and 82.8%.The specificity of cervical low level echo focus in diagnosis of cervical cancer and invasive cervical cancer were 100% and 94.8%,respectively.The specificity of abundant blood flow in dendritic form in diagnosis of invasive cervical cancer was 100%.Conclusions Invasive cervical cancer may present several specific features in TVCS images.TVCS examination is of high reliability in diagnosis of invasive cervical cancer,but is not so reliable in diagnosing precancerous lesion and preinvasive cancer.Combined with other auxiliary examinations,TVCS could be considered as one of the methods to diagnose cervical

  2. Investigação do valor da categoria diagnóstica de células epiteliais atípicas, de significado indeterminado, e origem indefinida da nomenclatura brasileira para laudos citopatológicos cervicais Investigation of the value of the atypical epithelial cells of undetermined significance and origin diagnostic category proposed by the Brazilian nomenclature for reporting cervical cytological diagnosis

    Directory of Open Access Journals (Sweden)

    Álvaro Piazzetta Pinto

    2006-04-01

    um achado raro e uma cuidadosa revisão da lâmina, na maioria dos casos, resultará no encontro de campos de AGUS. Vale ainda salientar a forte associação desse diagnóstico com áreas de má fixação nos esfregaços. Novos estudos sobre o assunto serão necessários. Com base nos achados do presente estudo, sugerimos que a persistência desse termo deva ser questionada em futuras revisões da nomenclatura nacional para laudos citopatológicos.BACKGROUND: In 2002, the Brazilian Nomenclature for Reporting Cervical Cytological Diagnosis was revised. A category of atypical epithelial cells of undetermined significance (AUS and another (sub-classification of atypical epithelial cells of undetermined significance and undetermined origin (AUSUO were introduced. Like atypical squamous cells of undetermined significance (ASCUS and atypical glandular cells of undetermined significance (AGUS, the diagnostic category AUSUO is controversial. Despite controversies, no previous national study had investigated its importance. OBJECTIVES: This study has the main objective of investigate the importance of the diagnostic category AUSUO. Another purpose is to contribute to spread the Brazilian Nomenclature for Reporting Cervical Cytological Diagnosis, by publishing it entirely. MATERIALS AND METHODS: The study resulted from the contribution of two private pathology and cytopathology laboratories. Cases diagnosed as ASCUS or AGUS with follow-up were selected from archives of cytopathology exams, collected from the period between 2000 and 2004. In total, 30 cases were selected and revised, being identified cytological diagnostic fields of ASIOI, ASCUS and AGUS. RESULTS: After revision, from the 30 cases, 26 were selected for the study. Among these, 19 presented cytological fields with diagnosis of only ASCUS and/or AGUS and were used as a control group. Only seven cases contained fields compatible with AUSUO, 4 (57,1% were associated with AGUS; 1 (14.3% with ASCUS; 1 (14.3% with both

  3. Genotypes of human papilloma virus in Sudanese women with cervical pathology

    Directory of Open Access Journals (Sweden)

    Tobi Khater

    2010-12-01

    Full Text Available Abstract Background Knowledge of the distribution of human papillomavirus (HPV genotypes among women with cervical lesion and in invasive cervical cancer is crucial to guide the introduction of prophylactic vaccines. There is no published data concerning HPV and cervical abnormalities in Sudan. This study aimed to define the prevalence of HPV and its subtypes in the cervical smears of women presenting with gynecological complains at Omdurman Military Hospital, Sudan. During the period between March 2003 and April 2004, 135 cervical smears collected from these women, were screened using cytological techniques, and analysed by PCR for (beta-globin and HPV DNA using gel electrophoresis and ELISA. Results Of these 135 smears, there were 94 (69.3% negative, 22 (16.3% positive for inflammation, 12(8.9 mild dyskaryosis, 5 (3.7 moderate dyskaryosis and 2 (1.8 severe dyskaryosis. There were 60.7% ß. globin positive samples for HPV indicating DNA integrity. HPV DNA was identified in three samples (2.2% by gel electrophoresis and. was positive in four samples (2.9% as single and multiple infections by PCR-ELISA. The high risk HPV types 16 and 58 were identified in one sample as a mixed infection. The low risk HPV types 40 and 42 were also found as a mixed infection in another patient. HPV types 58 and 42 were identified in the other two patients. Conclusion HPV type distribution in Sudan appears to differ from that in other countries. The HPV genotypes identified were not associated with cancer.

  4. Cytological artifacts masquerading interpretation

    Science.gov (United States)

    Sahay, Khushboo; Mehendiratta, Monica; Rehani, Shweta; Kumra, Madhumani; Sharma, Rashi; Kardam, Priyanka

    2013-01-01

    Background: Cytological artifacts are important to learn because an error in routine laboratory practice can bring out an erroneous result. Aims: The aim of this study was to analyze the effects of delayed fixation and morphological discrepancies created by deliberate addition of extraneous factors on the interpretation and/or diagnosis of an oral cytosmear. Materials and Methods: A prospective study was carried out using papanicolaou and hematoxylin and eosin-stained oral smears, 6 each from 66 volunteer dental students with deliberate variation in fixation delay timings, with and without changes in temperature, undue pressure while smear making and intentional addition of contaminants. The fixation delay at room temperature was carried out at an interval of every 30 minutes, 1 day and 1 week and was continued till the end of 1 day, 1 week, and 1 month, respectively. The temperature variations included 60 to 70°C and 3 to 4°C. Results: Light microscopically, the effect of delayed fixation at room temperature appeared first on cytoplasm followed by nucleus within the first 2 hours and on the 4th day, respectively, till complete cytoplasmic degeneration on the 23rd day. However, delayed fixation at variable temperature brought faster degenerative changes at higher temperature than lower temperature. Effect of extraneous factors revealed some interesting facts. Conclusions: In order to justify a cytosmear interpretation, a cytologist must be well acquainted with delayed fixation-induced cellular changes and microscopic appearances of common contaminants so as to implicate better prognosis and therapy. PMID:24648667

  5. Cervical Cancer

    Centers for Disease Control (CDC) Podcasts

    2007-03-06

    Did you know that cervical cancer rates differ by race/ethnicity and region? Or that cervical cancer can usually be prevented if precancerous cervical lesions are found by a Pap test and treated? Find out how getting regular Pap tests can save a woman's life.  Created: 3/6/2007 by National Breast and Cervical Cancer Early Detection Program.   Date Released: 4/25/2007.

  6. Restriction of human papillomavirus DNA testing in primary cervical screening to women above age 30

    DEFF Research Database (Denmark)

    Rebolj, Matejka; Njor, Sisse H; Lynge, Elsebeth

    2012-01-01

    Cervical screening with human papillomavirus (HPV) testing is less specific for high-grade cervical intraepithelial neoplasia (=CIN3) than cytology. The aim of this systematic review was to determine whether a restriction of HPV testing to women aged at least 30 years would eliminate the problem....

  7. Can we confidently diagnose pilomatricoma with fine needle aspiration cytology?

    Science.gov (United States)

    Wong, Yin-Ping; Masir, Noraidah; Sharifah, Noor Akmal

    2015-01-01

    Pilomatricomas can be confidently diagnosed cytologically due to their characteristic cytomorphological features. However, these lesions are rarely encountered by cytopathologists and thus pose a diagnostic dilemma to even experienced individuals, especially when the lesions are focally sampled. We describe two cases of histologically confirmed pilomatricoma. The first case is of a 13-year-old boy with posterior cervical 'lymphadenopathy', and the second one is of a 12-year-old girl with a lower cheek swelling. Both aspirates comprised predominantly atypical basal-like cells, with prominent nucleoli. 'Ghost cells' were readily identified by cell block in case two, but cell block in case one yielded no diagnostic material. In case two, pilomatricoma was accurately diagnosed pre-operatively. A cytological suspicion of a neoplastic process was raised in case one. Despite being diagnostically challenging, pilomatricoma can be diagnosed with careful observation of two unique cytological features of the lesions: (1) pathognomonic 'ghost cells' and (2) irregular, saw-toothed, loosely cohesive basaloid cells, with prominent nucleoli. The role of thorough sampling of the lesion, with multiple passes of various sites, cannot be overemphasized.

  8. Can we confidently diagnose pilomatricoma with fine needle aspiration cytology?

    Science.gov (United States)

    Wong, Yin-Ping; Masir, Noraidah; Sharifah, Noor Akmal

    2015-01-01

    Pilomatricomas can be confidently diagnosed cytologically due to their characteristic cytomorphological features. However, these lesions are rarely encountered by cytopathologists and thus pose a diagnostic dilemma to even experienced individuals, especially when the lesions are focally sampled. We describe two cases of histologically confirmed pilomatricoma. The first case is of a 13-year-old boy with posterior cervical 'lymphadenopathy', and the second one is of a 12-year-old girl with a lower cheek swelling. Both aspirates comprised predominantly atypical basal-like cells, with prominent nucleoli. 'Ghost cells' were readily identified by cell block in case two, but cell block in case one yielded no diagnostic material. In case two, pilomatricoma was accurately diagnosed pre-operatively. A cytological suspicion of a neoplastic process was raised in case one. Despite being diagnostically challenging, pilomatricoma can be diagnosed with careful observation of two unique cytological features of the lesions: (1) pathognomonic 'ghost cells' and (2) irregular, saw-toothed, loosely cohesive basaloid cells, with prominent nucleoli. The role of thorough sampling of the lesion, with multiple passes of various sites, cannot be overemphasized. PMID:25892955

  9. Cytological causes of blond psyllium for male sterility

    Directory of Open Access Journals (Sweden)

    Ashish G. Vala, Fougat. R.S. ,Roshni.S. and Vinay Kumar

    2011-03-01

    Full Text Available Cytological male sterility is a useful trait in plant breeding, especially in medicinal plants such as Cassia angustifolia, Commiphorawightii, Asparagus racemosus .Abnormalities appeared to be the cause of male sterility in plants, we carried out this research in anattempt to make clear the characteristics and inheritance of this male sterility. In Plantago ovata germplasm cytoplasmicabnormality was found to be the cause of abnormality. cytomixis varied from 2% to 48 % in plants. Maximum numbers of PMCscarrying B-chromosomes were recorded in male sterile plants of line JI-214(62%. No B-chromosomes were observed in fertile,sterile and partial male sterile line of JI-107 and maximum abnormal chromosomal segregation was recorded in JI-206. Thesecytological abnormality parameters can be used for hetrosis breeding and hybrid seed production to improve quality of psyllium.

  10. Testing Biopsy and Cytology Specimens for Cancer

    Science.gov (United States)

    ... articles window. My Saved Articles » My ACS » Testing Biopsy and Cytology Specimens for Cancer Download Printable Version [ ... on the topics below to get started. Testing Biopsy and Cytology Specimens for Cancer How is cancer ...

  11. New Molecular Tools for Efficient Screening of Cervical Cancer

    OpenAIRE

    Magnus von Knebel Doeberitz

    2001-01-01

    Cytological screening using the Pap-smear led to a remarkable reduction of the mortality of cervical cancer. However, due to subjective test criteria it is hampered by poor inter- and intra-observer agreement. More reproducible assays are expected to improve the current screening and avoid unnecessary medical intervention and psychological distress for the affected women. Cervical cancer arises as consequence of persistent high risk papillomavirus (HR-HPV) infections. Expression of two viral ...

  12. Economic burden of cervical cancer in Malaysia

    OpenAIRE

    Sharifa E. W. Puteh; Paul Ng; Aljunid, Syed M

    2008-01-01

    Cervical cancers form the second highest number of female cancers in Malaysia, imposing a substantial amount of cost burden on its management. However, an estimation of cost burden of abnormal smears, cervical pre-invasive and invasive diseases needs to be done to show how much spending has been allocated to the problem. An expert panel committee came up with the clinical pathway and management algorithm of  cervical pre invasive and invasive diseases from July-December 2006 Malaysia. An acti...

  13. Rationale and design of a multicenter prospective cohort study for the eVALuation and monitoring of HPV infections and relATEd cervical diseases in high-risk women (VALHIDATE study)

    International Nuclear Information System (INIS)

    Pap screening, an effective method for cervical cancer prevention, is now supported by molecular human papillomavirus (HPV) testing. Recently commercialised preventive vaccines also provide new tools for the primary prevention of cervical cancer. To determine appropriate prevention strategies, the Health General Direction, Lombardy Region, funded a project that aims to characterize and monitor HPV infections and related cervical diseases in high-risk women. VALHIDATE is a 5-year multicentre open prospective cohort study. It will recruit 7000 consenting women aged 13–65 years to provide information about the local biomolecular epidemiology of HPV infection and cervical diseases in high-risk women recruited from nine clinical centres and one faith-based organisation. The study will estimate the overall and type-specific prevalence of HPV infection and cervical abnormalities. It also aims to compare standard Pap screening with biomolecular screening, and to assist in the design of targeted regional prevention programs directed specifically at high-risk groups. Three groups of high-risk women: 1000 HIV-infected women (aged 26–65 years), 1000 recent migrant women (aged 26–65 years) and 3000 young women (aged 13–26 years) and 1 control group: 2000 women (aged 26–45 years) attending a spontaneous screening program, will be recruited. Sample sizes will be revised after the first year. Adult participants will undergo conventional cervical cytology, HPV DNA screening and genotyping. Paediatric participants will undergo HPV DNA testing and genotyping of urine samples. HPV DNA, cytological abnormalities and HPV types will be analysed according to demographic, epidemiological, behavioural, and clinical data collected in an electronic case report form. Overall and stratified prevalences will be estimated to analyse the associations between HPV infection and selected characteristics. Logistic regression models will be used to estimate crude and adjusted odds ratios. Cox

  14. Rationale and design of a multicenter prospective cohort study for the eVALuation and monitoring of HPV infections and relATEd cervical diseases in high-risk women (VALHIDATE study

    Directory of Open Access Journals (Sweden)

    Orlando Giovanna

    2012-05-01

    Full Text Available Abstract Background Pap screening, an effective method for cervical cancer prevention, is now supported by molecular human papillomavirus (HPV testing. Recently commercialised preventive vaccines also provide new tools for the primary prevention of cervical cancer. To determine appropriate prevention strategies, the Health General Direction, Lombardy Region, funded a project that aims to characterize and monitor HPV infections and related cervical diseases in high-risk women. Methods/design VALHIDATE is a 5-year multicentre open prospective cohort study. It will recruit 7000 consenting women aged 13–65 years to provide information about the local biomolecular epidemiology of HPV infection and cervical diseases in high-risk women recruited from nine clinical centres and one faith-based organisation. The study will estimate the overall and type-specific prevalence of HPV infection and cervical abnormalities. It also aims to compare standard Pap screening with biomolecular screening, and to assist in the design of targeted regional prevention programs directed specifically at high-risk groups. Three groups of high-risk women: 1000 HIV-infected women (aged 26–65 years, 1000 recent migrant women (aged 26–65 years and 3000 young women (aged 13–26 years and 1 control group: 2000 women (aged 26–45 years attending a spontaneous screening program, will be recruited. Sample sizes will be revised after the first year. Adult participants will undergo conventional cervical cytology, HPV DNA screening and genotyping. Paediatric participants will undergo HPV DNA testing and genotyping of urine samples. HPV DNA, cytological abnormalities and HPV types will be analysed according to demographic, epidemiological, behavioural, and clinical data collected in an electronic case report form. Overall and stratified prevalences will be estimated to analyse the associations between HPV infection and selected characteristics. Logistic regression models

  15. A pedigree of cervical stenosis, brachydactyly, syndactyly, and hyperopia.

    Science.gov (United States)

    Iida, H; Shikata, J; Yamamuro, T; Takeda, N; Ueba, Y

    1989-10-01

    Cervical myelopathy due to developmental cervical canal stenosis occurred in a 13-year-old boy. The patient's father and aunt also had an abnormally small cervical canal, although both were asymptomatic. The patient and his family had many congenital anomalies including hereditary brachydactyly, syndactyly, and hyperopia. The association of these anomalies seems not to have been previously reported in the literature. PMID:2551554

  16. Analysis of cervical diseases in 2 333 married women aged 35-59 years%2333例35~59岁已婚妇女宫颈疾病分析

    Institute of Scientific and Technical Information of China (English)

    梁海虹; 徐旦; 吴美飞

    2015-01-01

    Objective To understand the incidence status of cervical diseases among rural (fishery) women in Xiangshan area, so as to improve the early diagnosis and treatment of cervical cancer and improve the self health care consciousness and health level of rural ( fishery) women.Methods Cervical cells were collected by pelvic examination to perform liquid based thin-layer cytology test ( TCT) , and colposcopy examination, HPV test and histopathological examination were further performed for cases with abnormal cytological results. Results Gynecological examination showed that 57.35%of rural ( fishery) women were diagnosed with cervical diseases and the incidence of cervical erosion was highest (21.35%).TCT examination revealed that the incidence of abnormal cervical cytology results, precancerous lesions and cervical cancer was 3.26%, 120/10 000 and 8.57/10 000, respectively.Conclusion TCT diagnosis revealed by TBS reporting system was superior to traditional cervical smear pap cytology report in detecting of precancerous lesions.Women of childbearing age ranging 40-50 years are high-risk group developing cervical cancer, and the cases with persistent infection of HPV are the objects of key intervention and testing.Strengthening rural ( fishery ) women ’ s health education and improving their self health care consciousness will contribute to the early prevention of cervical disease.%目的:了解象山海岛地区农(渔)村妇女宫颈疾病的发病状况,提高宫颈癌的早诊和早治率,提升广大农(渔)村妇女的自我保健意识和健康水平。方法采用妇科内诊检查,采集宫颈细胞进行液基薄层细胞学检查( TCT),对细胞学检查异常的患者进一步行阴道镜检查、人乳头瘤病毒( HPV)检测、宫颈组织病理学检查。结果通过妇科检查发现57.35%的农(渔)村妇女患有宫颈疾病,其中以宫颈糜烂发病率最高(21.35%);通过TCT检查发现

  17. TCT与HR-HPV联合检测对宫颈癌筛查的意义%Role of combined use of TCT and HR-HPV testing in screening cervical cancer

    Institute of Scientific and Technical Information of China (English)

    胡静熠; 吴江平

    2012-01-01

    Objective To investigate the clinical significance of thin prep liquid-based cytology test(TCT) combined with high-risk human papillomavirus (HR-HPV) in the diagnosis of cervical cancer and cervical early lesions. Methods Cervical cytological examination and HR-HPV testing were carried out by TCT and flow-through hybrization and gene chip(HybriMax) in 520 women. Results The cytological abnormity was reported by TCT in 148 cases with an inspection rate of 28. 5%(148/520) ,of whom atypical squamous celKASC) was found in 105 cases,low-grade squamous intraepithelial lesion(LSIL) in 32 cases,HSIL high-grade squamous intraepithelial lesion(HSIL) in 10 cases,and cervical squamous cells cancer(SCC) in 1 case. In the positive cases reported by TCT test, HR-HPV positive was detected in 77. l%(81/105) for ASC,84. 4%(27/32) for LSIL,90. 0%(9/10) for HSIL, and 100.0% (1/1) for SCC. The positive rate of HR-HPV was increased as the grade of cervical cytology increased. Conclusion The positive rate of HR-HPV is closely associated with the grade of cervical cytological abnormity. The false negative rate of TCT test can be reduced by HR-HPV detection. Combined examination of HR-HPV and TCT test is valuable in the early diagnosis of cervical cancer and cervical lesions.%目的 探讨液基薄层细胞学技术(TCT)与高危型人乳头状瘤病毒(HR-HPV)联合检测对筛查宫颈癌和宫颈早期病变的临床意义.方法 宫颈癌普查的体检者520例,采用TCT和HR-HPV分型检测,后者用导流杂交基因芯片技术(HybriMax).分析联合检测对宫颈早期病变筛查的价值.结果 TCT检测中有148例出现细胞学异常,检出率28.5%(148/520);其中,非典型鳞状细胞癌(ASC)105例,低度鳞状上皮内病变(LSIL)32例,高度鳞状上皮内病变(HSIL)10例,宫颈鳞状细胞癌(SCC)1例.HR-HPV阳性率39.8%(207/520).TCT阳性结果病例中,HR-HPV的阳性率分别为ASC 77.1%(81/105),LSIL 84.4%(27/32),HSIL 90.0%(9/10),SCC 100.0%(1/1).HR

  18. Impact of intravenous acetaminophen therapy on the necessity of cervical spine imaging in patients with cervical spine trauma

    Institute of Scientific and Technical Information of China (English)

    Koorosh Ahmadi; Amir Masoud Hashemian; Elham Pishbin; Mahdi Sharif-Alhoseini; Vafa Rahimi-Movaghar

    2014-01-01

    Objective:We evaluated a new hypothesis of acetaminophen therapy to reduce the necessity of imaging in patients with probable traumatic cervical spine injury.Methods:Patients with acute blunt trauma to the neck and just posterior midline cervical tenderness received acetaminophen (15 mg/kg) intravenously after cervical spine immobilization.Then,all the patients underwent plain radiography and computerized tomography of the cervical spine.The outcome measure was the presence of traumatic cervical spine injury.Sixty minutes after acetaminophen infusion,posterior midline cervical tendemess was reassessed.Results:Of 1 309 patients,41 had traumatic cervical spine injuries based on imaging.Sixty minutes after infusion,posterior midline cervical tenderness was eliminated in 1 041 patients,none of whom had abnormal imaging.Conclusion:Patients with cervical spine trauma do not need imaging if posterior midline cervical tendemess is eliminated after acetaminophen infusion.This analgesia could be considered as a diagnostic and therapeutic intervention.

  19. DAPK1, MGMT and RARB promoter methylation as biomarkers for high-grade cervical lesions

    OpenAIRE

    Sun, Yin; Li, Shu; Shen, Keng; Ye, Shuang; Cao, Dongyan; Yang, Jiaxin

    2015-01-01

    Gene promoter methylation may be used a potential biomarker for detecting solid tumor including cervical cancer. Here, we used methylation sensitive-high resolution melting (MS-HRM) analysis to detecting promoter methylation ratios of DAPK1, MGMT and RARB gene in patients with different cervical disease grade. The detection of gene promoter methylation was conducted in two hundred fifty patients’ samples including normal cytology (n=48), cervical intraepithelial neoplasia grade 1 (CIN1, n=54)...

  20. Cervical incompetence: preliminary evaluation with MR imaging.

    Science.gov (United States)

    Hricak, H; Chang, Y C; Cann, C E; Parer, J T

    1990-03-01

    The ability of magnetic resonance (MR) imaging to demonstrate cervical incompetence in nonpregnant women was investigated in a prospective study of 41 volunteers referred in random order. These included 20 patients with normal cervices, 11 with cervical incompetence of traumatic or congenital origin, and 10 with clinically small cervices due to in utero diethylstilbestrol (DES) exposure. On MR images of the normal patients, cervical length was 33.0 mm +/- 1.0 (mean +/- standard error of the mean) and the width of the internal cervical os was 3.3 mm +/- 0.1. In patients with cervical incompetence, the cervical length did not significantly differ from those in the normal group. However, the internal cervical os was significantly wider (4.5 mm +/- 0.3, P less than .001), and localized irregularity of the endocervical canal was demonstrated in two patients. The MR appearance of the cervical stroma varied from normal, uniformly low signal intensity (n = 4) to uniformly (n = 3) or partially (n = 4) medium-to-high signal intensity on T2-weighted images. In the patients with in utero DES exposure, the mean length of the cervical canal (22.9 mm +/- 1.7) was significantly shorter than that of the normal group. The width of the internal cervical os and the MR signal intensity of the cervical stroma were normal. In summary, MR findings of a cervical length shorter than 3.1 mm (95% confidence limit), an internal cervical os wider than 4.2 mm (95% confidence limit), or abnormal signal intensity in the cervical stroma are highly suggestive of incompetent cervix and should assist in planning further therapeutic decisions. PMID:2305065

  1. Cervical cancer screening at crossroads

    DEFF Research Database (Denmark)

    Lynge, Elsebeth; Rygaard, Carsten; Baillet, Miguel Vazquez-Prada;

    2014-01-01

    ) demonstrated that HPV testing provides better protection against cervical cancer than cytology, but it requires extra repeated testing. HPV vaccination RCTs, furthermore, have proved that HPV vaccination protects against vaccine-type high-grade CIN in women vaccinated prior to sexual activity, but less so...... cancer case. The discovery of human papillomavirus (HPV) as the cause of cervical cancer dramatically changed perspectives for disease control. Screening with HPV testing was launched around 1990, and preventive HPV vaccination was licensed in 2006. Long-term randomized controlled trials (RCT...... in women vaccinated later. The challenge now is therefore to find an algorithm for screening of a heterogeneous population including non-vaccinated women; women vaccinated prior to start of sexual activity; and women vaccinated later....

  2. Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil

    Directory of Open Access Journals (Sweden)

    Geni NL Camara

    2003-10-01

    Full Text Available As a contribution to the public health authorities in planning prophylactic and therapeutic vaccine strategies, we describe the prevalence of human papillomavirus (HPV types in women presenting abnormal cytological results in Pap smear screening tests in the Federal District, Central Brazil. We studied 129 cervical scraping samples from women whose cytological tests showed either pre-neoplastic or neoplastic lesions. Amplification of HPV DNA was performed by polymerase chain reaction using consensus primers MY09 and MY11 followed by identification of isolates by restriction fragment length polymorphism. We detected HPV DNA in 62% of the samples, including HPV-16 in 43.8%, HPV-58 in 12.5%, HPV-31 in 10%, HPV-53 in 6.3%, each of HPV-18 and HPV-33 in 3.8% of the isolates. Other types (HPV-35, -52, -66, -CP8304, -6, -11, and -CP8061 were less frequent (= or < 2.5% each. The prevalence of HPV-58 was relatively higher in this population than in data in South America, but similar to results obtained in other studies in Latin America, Europe, and Eastern Asia. Case-control studies need to be carried out to establish the association between the prevalence of HPV types specially the less frequent high-risk types and cervical cancer.

  3. Cytological evaluation of enlarged lymph nodes in metastatic disease: A hospital-based assessment

    Directory of Open Access Journals (Sweden)

    Ghazala Mehdi

    2015-01-01

    Full Text Available Background: Lymphadenopathy is a heterogeneous entity with many underlying causes, ranging from self-limiting benign disease to severe neoplastic proliferations. Fine-needle aspiration is a cost-effective and reliable tool for initial investigation of enlarged lymph nodes. Materials and Methods: A study was conducted on 326 patients with metastatic disease in order to evaluate the efficacy of cytology in diagnosing malignancies metastatizing to the lymph node and predicting their primary origin as well as to find the relative frequency of different malignancies. The findings were also correlated with histopathology. Results: Cervical lymph nodes were the most frequently involved group, followed by axillary, supraclavicular, and inguinal lymph nodes. Squamous cell carcinoma (SCC and adenocarcinoma were the most common cytological diagnosis. Among the cases with known primary tumors, head and neck was the most common site followed by breast carcinoma. Most common lymph node group to be aspirated in cases with unknown primary was cervical lymph node, and SCC was most frequently diagnosed cases. Sensitivity and positive predictive value of cytological diagnosis were calculated to be 100% and 93.1%, respectively. Conclusion: Fine-needle aspiration cytology has a very high sensitivity and positive predictive value and hence, a presumptive diagnosis can be made along with the detection of the primary site in case of metastatic disease.

  4. Human papillomavirus testing and genotyping in cervical screening

    DEFF Research Database (Denmark)

    Rebolj, Matejka; Lynge, Elsebeth; Bonde, Jesper

    2011-01-01

    Mass vaccination against human papillomavirus (HPV) genotypes 16 and 18 will, in the long term, reduce the incidence of cervical cancer, but screening will remain an important cancer control measure in both vaccinated and unvaccinated women. Since the 1960s, cytology screening has helped to reduc...

  5. Human papillomavirus type-specific prevalence in the cervical cancer screening population of Czech women.

    Directory of Open Access Journals (Sweden)

    Ruth Tachezy

    Full Text Available BACKGROUND: Infection with high-risk human papillomavirus (HPVtypes has been recognized as a causal factor for the development of cervical cancer and a number of other malignancies. Today, vaccines against HPV, highly effective in the prevention of persistent infection and precancerous lesions, are available for the routine clinical practice. OBJECTIVES: The data on the prevalence and type-specific HPV distribution in the population of each country are crucial for the surveillance of HPV type-specific prevalence at the onset of vaccination against HPV. METHODS: Women attending a preventive gynecological examination who had no history of abnormal cytological finding and/or surgery for cervical lesions were enrolled. All samples were tested for the presence of HPV by High-Risk Hybrid Capture 2 (HR HC2 and by a modified PCR-reverse line blot assay with broad spectrum primers (BS-RLB. RESULTS: Cervical smears of 1393 women were analyzed. In 6.5% of women, atypical cytological findings were detected. Altogether, 28.3% (394/1393 of women were positive for any HPV type by BS-RLB, 18.2% (254/1393 by HR HC2, and 22.3% (310/1393 by BS-RLB for HR HPV types. In women with atypical findings the prevalence for HR and any HPV types were significantly higher than in women with normal cytological findings. Overall, 36 different HPV types were detected, with HPV 16 being the most prevalent (4.8%. HPV positivity decreased with age; the highest prevalence was 31.5% in the age group 21-25 years. CONCLUSIONS: Our study subjects represent the real screening population. HPV prevalence in this population in the Czech Republic is higher than in other countries of Eastern Europe. Also the spectrum of the most prevalent HPV types differs from those reported by others but HPV 16 is, concordantly, the most prevalent type. Country-specific HPV type-specific prevalences provide baseline information which will enable to measure the impact of HPV vaccination in the future.

  6. Cervical radiculopathy.

    Science.gov (United States)

    Iyer, Sravisht; Kim, Han Jo

    2016-09-01

    Cervical radiculopathy is a common clinical scenario. Patients with radiculopathy typically present with neck pain, arm pain, or both. We review the epidemiology of cervical radiculopathy and discuss the diagnosis of this condition. This includes an overview of the pertinent findings on the patient history and physical examination. We also discuss relevant clinical syndromes that must be considered in the differential diagnosis including peripheral nerve entrapment syndromes and shoulder pathology. The natural history of cervical radiculopathy is reviewed and options for management are discussed. These options include conservative management, non-operative modalities such as physical therapy, steroid injections, and operative intervention. While the exact indications for surgical intervention have not yet been elucidated, we provide an overview of the available literature regarding indications and discuss the timing of intervention. The surgical outcomes of anterior cervical decompression and fusion (ACDF), cervical disc arthroplasty (CDA), and posterior cervical foraminotomy (PCF) are discussed. PMID:27250042

  7. Cervical Cancer Screening Among Homeless Women of New York City Shelters.

    Science.gov (United States)

    Asgary, Ramin; Alcabes, Analena; Feldman, Rebecca; Garland, Victoria; Naderi, Ramesh; Ogedegbe, Gbenga; Sckell, Blanca

    2016-06-01

    Introduction Homeless persons have minimal opportunities to complete recommended cancer screening. The rates and predictors of cervical cancer screening are understudied among homeless women in the US. Methods We enrolled 297 homeless women 21-65 years old residing in 6 major New York City shelters from 2012 to 2014. We used a validated national survey to determine the proportion and predictors of cervical cancer screening using cytology (Pap test). Results Mean age was 44.72 (±11.96) years. Majority was Black, heterosexual, single, with high school or lower education; 50.9 % were smokers and 41.7 % were homeless more than a year. Despite a 76.5 % proportion of self-reported Pap test within the past 3 years, 65 % of women assumed their Pap test results were normal or did not get proper follow up after abnormal results. Forty-five-point-nine percent of women did not know about frequency of Pap test or causes of cervical cancer. Lower proportion of up-to-date Pap test was associated with lack of knowledge of recommended Pap test frequency (p homeless women was similar to a national sample. However, the majority of women surveyed were not aware of their results, received limited if any follow up and had significant education gaps about cervical cancer screening. We recommend improved counseling and patient education, patient navigators to close screening loops, and consideration of alternative test-and-treat modalities to improve effective screening. PMID:26649876

  8. The potential therapeutic targets for cervical cancer

    Directory of Open Access Journals (Sweden)

    L Priyanka Dwarampudi

    2013-01-01

    Full Text Available In case of invasive cervical carcinoma several molecular events were reported and these molecular events resulting in multiple genetic abnormalities. In order to control these tumors multiple molecular therapeutic targets are needed with different molecular mechanisms. Unfortunately, these molecular targets were in early stages of development. Because of less degree of success of conventional therapeutics for late stages of cervical cancer and lowering of prognosis of patients there is an increase in interest for the development of potential therapeutic targets for cervical cancer. This review article emphasizes the current molecular targeted agents; with special attention to estrogen receptors for human papilloma virus infected cervical cancer.

  9. Design and methods of a population-based natural history study of cervical neoplasia in a rural province of Costa Rica: the Guanacaste Project.

    Science.gov (United States)

    Herrero, R; Schiffman, M H; Bratti, C; Hildesheim, A; Balmaceda, I; Sherman, M E; Greenberg, M; Cárdenas, F; Gómez, V; Helgesen, K; Morales, J; Hutchinson, M; Mango, L; Alfaro, M; Potischman, N W; Wacholder, S; Swanson, C; Brinton, L A

    1997-05-01

    This paper reports on the enrollment phase of a population-based natural history study of cervical neoplasia in Guanacaste, a rural province of Costa Rica with consistently high rates of invasive cervical cancer. The main goals of the study are to investigate the role of human papillomavirus (HPV) infection and its co-factors in the etiology of high-grade cervical neoplasia, and to evaluate new cervical cancer screening technologies. To begin, a random sample of censal segments was selected and enumeration of all resident women 18 years of age and over was conducted with the aid of outreach workers of the Costa Rican Ministry of Health. Of the 10738 women who were eligible to participate, 10049 (93.6%) were interviewed after giving written informed consent. After the interview on cervical cancer risk factors was administered, a pelvic examination was performed on those women who reported previous sexual activity. The pelvic examination included a vaginal pH determination and collection of cervical cells for cytologic diagnosis using three different techniques. Additional cervical cells were collected for determination of the presence and amount of DNA from 16 different types of HPV, and two photographic images of the cervix were taken and interpreted offsite by an expert colposcopist. Finally, blood samples were collected for immunologic and micronutrient assays. Women with any abnormal cytologic diagnosis or a positive Cervigram, as well as a sample of the whole group, were referred for colposcopy, and biopsies were taken when lesions were observed. The enrollment screening will serve as the basis for a prevalent case-control study, and the members of the cohort free from serious disease will be followed actively, at intervals of no more than a year, to study the natural history of HPV infection and the origins of high-grade squamous intraepithelial lesions (HSIL). Details of the field operation are outlined, with particular reference to the realization of this

  10. Detection and typing of human papillomaviruses by restriction fragment length polymorphism in women with different cytology

    Directory of Open Access Journals (Sweden)

    Vesna Tripković,

    2010-02-01

    Full Text Available Aim The aim of this study was to determine a percentage of high risk genotypes which are included in the current vaccines in women with different cytology and on the basis of obtained results to evaluatethe protective activity of the current vaccines. Methods Endocervical swabs were taken from 70 women with different cervical cytology (25 women with CIN 1, 25 women with CIN 2/3 and 20 women with negative cytology. The samples were tested by PCR method using MY09/MY11 primers to determinate the HPV status, especially the percentage of high risk genotypes which are not included in the current vaccines. Genotyping was done by RFLP method. Results In as much as 38.8% of all samples high risk genotypes (hrHPV, not included in the current vaccine, were detected; in a group of women with normal cytology hrHPV genotypes were found in 29.9% samples. HrHPV were found in 47%, and 64% of samples taken from women with normal cytology and CIN 1, respectively, while in women with CIN 2 /3 hrHPV were found in 94%. Conclusion These results indicate that in more than in one third of tested women the current available vaccines would be of minimal protective activity, but further studies which should include more women are needed.

  11. Screening for cervical cancer: new alternatives and research Detección oportuna de cáncer cervical: nuevas alternativas y pautas de investigación

    OpenAIRE

    Lörincz, Attila T.

    2003-01-01

    Evidence for the clinical utility of human papillomavirus (HPV) DNA testing has increased over the years and has now become very convincing. Some specific uses of HPV detection are a) triage of women with cytological determinations of atypical squamous cells of undetermined significance (ASC-US) and related management strategies, b) as a marker for test of cure post-treatment, and c) most importantly, as an adjunct to cytology in routine cervical disease screening programs. There are many stu...

  12. CYTOLOGICAL DIAGNOSIS OF GELATINOUS ASCITES

    Directory of Open Access Journals (Sweden)

    Samith

    2013-04-01

    Full Text Available ABSTRACT: Pseudomyxoma peritonei (PMP is a condition characte rized by the accumulation of mucinous material in the peritoneal cavity (gela tinous ascites. Pseudomyxoma peritonei is an uncommon condition in which mucinous ascites causes progressive abdominal distension and gastrointestina l dysfunction. We present a case of 44 year old female who presented with gradually progres sive abdominal distension since 6 months. An ascitic tap was performed and the fluid wa s sent for cytological study. Cytology showed pools of acellular mucin. On laparotomy there was mucinous material in the abdomen that was evacuated. Appendix and omentum were found t o be thickened, hence appendicectomy was done and omental biopsy taken. Histopathological diagnosis of well differentiated mucinous adenocarcinoma of appendix with rupture and mucinous ascitis was made.

  13. Vulval schwannoma: A cytological diagnosis

    Directory of Open Access Journals (Sweden)

    Das Subhashish

    2008-01-01

    Full Text Available Schwannomas are the most common peripheral nerve sheath tumors, the frequent sites of involvement being the head and neck, the flexor aspect of the limbs, especially near the elbow, wrist, knee, and trunk. Involvement of the female genital tract is extremely rare. We present one such rare case of a solitary vulval schwannoma which was diagnosed by fine needle aspiration cytology in a 48 year-old female, and confirmed by histopathological examination.

  14. Incidence of cervical dysplasia and cervical cancer in women living with HIV in Denmark

    DEFF Research Database (Denmark)

    Thorsteinsson, Kristina; Ladelund, Steen; Jensen-Fangel, Søren;

    2014-01-01

    and hazard ratios (HRs) for time from inclusion to first cervical intraepithelial neoplasia (CIN)/ICC and time from first normal cervical cytology to first CIN/ICC were estimated. Sensitivity analyses were performed to include prior screening outcome, screening intensity and treatment of CIN...... in Denmark. METHODS: We studied a nationwide cohort of WLWH and a cohort of age-matched females from the general population in the period 1999-2010. Pathology samples were obtained from The Danish Pathology Data Bank containing nationwide records of all pathology specimens. The cumulative incidence...

  15. Clinical effect of semen-storing cervical cap on artificial insemination outcome in patients with abnormal anatomical position of cervix%储精式宫颈帽用于宫颈解剖位置异常人工授精的临床效果观察

    Institute of Scientific and Technical Information of China (English)

    陶国振; 胥玉梅; 李娟; 孙林; 刘锦云; 于建春

    2012-01-01

    目的:观察储精式宫颈帽用于宫颈解剖位置异常人工授精的临床效果.方法:将宫颈解剖位置异常导致不孕的妇女66例随机分成3组,实验组采用储精式宫颈帽夫精人工授精,对照A组采用宫颈管夫精人工授精,对照B组采用宫腔夫精人工授精,比较各组周期妊娠率.结果:周期妊娠率实验组(21.86%,14/65)高于对照A组(8.06%,5/62)(P <0.05),与对照B组(23.21%,13/56)比较无统计学差异(P>0.05).结论:储精式宫颈帽夫精人工授精治疗宫颈解剖位置异常所致不孕与宫腔夫精人工授精临床效果一致,明显高于宫颈管夫精人工授精,是一种简单、方便和有效的人工授精方法.%Objective: To explore the clinical use of self - made semen - storing cervical cap on the outcome of artificial insemination with husband sperm in infertile women with abnormal anatomical position of the cervix. Methods; Sixty - six infertile women with abnormal anatomical position of the cervix were prospectively randomized to 3 groups; the experimental group, control group A and B. Women in the experimental group received underwent artificial insemination with semen - storing cervical cap. And those in control group A and B received cervical artificial insemination and intrauterine insemination, respectively. The pregnancy rate was evaluated. Results o The pregnancy rate of the experimental group and was 21.86% (14/65), significantly higher than that of control group A (8.06% , 5/62, P0.05). Conclusion; The clinical effect of artificial insemination with semen - storing cervical cap is similar with that of intrauterine insemination and higher than that of cervical artificial insemination for patients with abnormal anatomical position of the cervix. It may be a simple, convenient and effective method for artificial insemination.

  16. The impact of HPV vaccination on future cervical screening

    DEFF Research Database (Denmark)

    Hestbech, Mie Sara; Lynge, Elsebeth; Kragstrup, Jakob;

    2015-01-01

    OBJECTIVES: To explore the interplay between primary and secondary prevention of cervical cancer by estimating future screening outcomes in women offered human papillomavirus (HPV) vaccination when they were sexually naïve. DESIGN: Estimation of outcome of liquid-based cytology screening for a post......-HPV vaccination cohort using pre-vaccination screening data combined with HPV vaccination efficacy data reported in the literature. SETTING: Denmark. DATA: The number of screening diagnoses at first screen in a pre-vaccination birth cohort was multiplied by reported risk reductions expected for women who were...... vaccinated for HPV before sexual debut. All identified studies were reviewed by two authors, and weighted pooled estimates of vaccine efficacies were used. MAIN OUTCOME MEASURES: Proportions of positive and false-positive cervical cytologies and positive predictive value (PPV) were calculated using cervical...

  17. Review on the medical and health economic evidence for an inclusion of colposcopy in primary screening programs for cervical cancer

    Directory of Open Access Journals (Sweden)

    von der Schulenburg, Johann-Matthias

    2007-08-01

    Full Text Available Introduction: With 3.2% of all cancer cases in 2002, cervical carcinoma is the tenth most common cancer in Germany and causes 1.8% of all cancer deaths in women in Germany. To date diagnosis in Germany solely has been based on cervical cytology which has been criticised due to its low sensitivity and consequently high rate of false negative results. Objectives: How does colposcopy compare to cytological tests in terms of sensitivity and specificity, and what may be the effects of changes in screening for cervical carcinoma in Germany? Is there health economic evidence that may foster an inclusion of colposcopy into national screening programms? Methods: A systematic literature review was performed, including studies that compared colposcopy to cervical cytology in terms of sensitivity and specificity. In addition, a systematic review of the relevant health economic literature was performed to analyse cost-effectiveness issues relevant to the German setting. Results: A total of four studies fulfilled the inclusion criteria, of which only two were of high methodologic quality. In all studies, the sensitivity of colposcopy was lower than that of cytology. In three studies the specificity of colposcopy was lower than that of cytology, in one study specificity of colposcopy and cytology was similar. No health economic data suggesting positive effects of adding colposcopy in primary screening could be identified. Discussion: Only few studies have compared the test criteria of colposcopy with those of cytology for screening in cervical cancer. In all studies, sensitivity of colposcopy was even lower than the sensitivity of cytology, which has been critisized because of its low sensitivity. Conclusion: Based on the present data, an inclusion of colposcopy in primary cervical cancer screening programmes can not be recommended.

  18. Impression Cytology with Transfer in xerophthalmia and conjunctival diseases.

    Science.gov (United States)

    Resnikoff, S; Luzeau, R; Filliard, G; Amedee-Manesme, O

    1992-11-01

    During a countrywide survey, we assessed the prevalence of clinical signs of xerophthalmia and of major conjunctival diseases in a randomized sample of 2,445 subjects representative of the population of the Republic of Djibouti. On a part of this sample, conjunctival Impression Cytology with Transfer (ICT) test and a plasma retinol determination were performed. Xerophthalmia as a public health problem was displayed by clinical signs (Bitot's spots, corneal scars among preschool children), low plasma retinol levels and ICT test results: 9.3% with deficient cytology in the rural area and 12.3% in the urban one (age-standardized rates). Results of ICT were related to age (p children but also up to 15 years. Moreover, ICT results are influenced by conjunctival diseases: compared to age-matched controls, there were more abnormal cytologies among patients with trachomatous inflammation (p = 0.025), conjunctivitis (p = 0.024) or Limbal Vernal Keratoconjunctivitis (p = 0.015). Thus ICT shouldn't be performed among children with conjunctival diseases. In the region under study conjunctival diseases had high rates of prevalence: 16.4% of trachomatous scarrings in the urban area (standardized rate), 8% of conjunctivitis among rural preschool children, and 5% of Limbal Vernal Keratoconjunctivitis among children between 5 and 14 years in both areas. PMID:1490836

  19. Operations for cervical incompetence.

    Science.gov (United States)

    Branch, D W

    1986-06-01

    At present, cervical cerclage is indicated in those patients with a classic history of cervical incompetence. For the majority of these patients, a postconceptional cerclage procedure is better suited because it is done after the fetus has been evaluated ultrasonographically for obvious abnormalities and after the risk for spontaneous abortion is past. The greatest experience is with the Shirodkar and the McDonald procedures; allowing for operator differences, neither appears to have an important advantage. The transabdominal cervicoisthmic cerclage may be a useful postconceptional procedure in the selected patient whose cervix is either markedly foreshortened, deeply lacerated, or infected. Preconceptional cerclages are best reserved for those rare patients in whom a markedly foreshortened incompetent cervix is associated with early second-trimester pregnancy losses (preconceptional isthmic cerclage) or in whom the cervix has a single, identifiable scar or deep laceration extending through the internal os (Lash procedure). PMID:3522005

  20. 42 CFR 493.1274 - Standard: Cytology.

    Science.gov (United States)

    2010-10-01

    ... a diagnosis of HSIL, adenocarcinoma, or other malignant neoplasm for which histology results were available for comparison; (v) Gynecologic cases where cytology and histology are discrepant; and...

  1. Conjunctival impression cytology: bright hope of children.

    Science.gov (United States)

    1991-01-01

    A practical method of screening for pre-clinical xerophthalmia due to vitamin A deficiency, called conjunctival impression cytology (CIC), is described as it is being used in a training stage in the Philippines. The noninvasive technic consists of touching the conjunctiva with a filter paper disc, and fixing and staining the disc on a slide for histology. Normally goblet cells with mucin spots are seen among sheets of epithelial cells. In abnormal conjunctiva from vitamin A deficient individuals, the epithelial cells are enlarged, and goblet cells are lacking. These specimens may be obtained from areas of the conjunctiva that appear clinically normal. The equipment needed is millipore paper, a hand-held suction pump with 5 feet of tubing, tissue or gauze, screw-top vials, labels, fixative, Papanicolaou stain, and a microscope. Vitamin A supplements can be given to affected children, or to the whole population at risk. With CIC training materials donated by International Center for Epidemiologic and Preventive Ophthalmology (ICEPO) at the Wilmer Institute, and the School of Hygiene and Public Health of the Johns Hopkins University, Baltimore, the 1st training class was certified by the Nutrition Center of the Philippines. Twice yearly training of physicians and technologists has been recommended.

  2. Fine needle cytology of Kaposi's sarcoma in heterosexual male

    Directory of Open Access Journals (Sweden)

    Anjali R. Dhote

    2014-04-01

    Full Text Available Kaposi's sarcomas the most common malignancy associated with Human Herpesvirus-8 (HHV8 infection. Though name is sarcoma but it is low grade vascular neoplasm. It is the tumour which arises from endothelial lining of vessels as well as lymphatic channels. So it involved all sites such as skin, Gastro intestine, lungs along with lymph nodes. We are presenting one such case of 65 year immunocompromised Indian male presented with multiple non blanching reddish bluish nodules on all extremities, chest, back with submandibular and cervical lymphadenopathy. Fine needle aspiration cytology (FNAC was performed and diagnosis was given low grade spindle cell neoplasm consistent with Kaposi's sarcoma which was confirmed on histopathology as Kaposi's sarcoma. [Int J Res Med Sci 2014; 2(2.000: 789-791

  3. Comprehensive evaluation of cervical cancer screening programs: the case of Colombia

    OpenAIRE

    Raúl Murillo; Carolina Wiesner; Ricardo Cendales; Marion Piñeros; Sandra Tovar

    2011-01-01

    OBJECTIVE: To identify critical screening program factors for reducing cervical cancer mortality in Colombia. MATERIAL AND METHODS: Coverage, quality, and screening follow-up were evaluated in four Colombian states with different mortality rates. A case-control study (invasive cancer and healthy controls) evaluating screening history was performed. RESULTS: 3-year cytology coverage was 72.7%, false negative rate 49%, positive cytology follow-up 64.2%. There was no association between screenin...

  4. 广东粤北瑶族地区农村妇女宫颈癌筛查的临床分析%Cervical cancer screening in rural women of Yao’s area in northern Guangdong:a clinical analysis

    Institute of Scientific and Technical Information of China (English)

    陈美玲

    2015-01-01

    Objective:To investigate cervical precancerous lesions and clinical features of cervical cancer in rural women of Yao ethnic region in northern Guangdong. Methods:A total of 1838 rural women,who received cervical cancer screening in Maternal and Child Health Hospital of Yao Autonomous County of Ruyuan,Shaoguan city between June 2014 and November 2014,were included as subjects in the study,using cervical liquid based cytology examination and cervical biopsy. Results: The abnormal rate of cervical liquid based cytology examination and cervical intraepithelial neoplasia( CIN)rate of cervical biopsy pathological examination in women aged 35~44 were significantly higher than those in women aged 45~ 54,or 55~ 64 year old(P﹤0.05). The incidence of cervical cancer in women aged 45~54 was significantly higher than that in women aged 35~44 or 55~ 64 year old(P﹤0.05). Conclusion:Among rural women of Yao ethnic region in northern Guangdong, high rates of abnormal cervical liquid based cytology and CIN are found in women aged 35 ~ 44;and the occurrence of cervical cancer is frequently found in women aged 45~54 year old,suggesting the need for relevant departments to strengthen and improve the disease screening system in ethnic minority areas with early diagnosis and treatment,so as to lower the incidence and mortality of cervical cancer.%目的:分析广东粤北瑶族地区农村妇女宫颈癌前病变及宫颈癌临床特征。方法:选择2014年6-11月在韶关市乳源瑶族自治县妇幼保健院接受宫颈癌筛查的1838名农村妇女作为研究对象,进行宫颈液基细胞学检查和宫颈活组织检查。结果:35~44岁女性宫颈液基细胞学异常率、宫颈活检病理检查CIN 病变率显著高于45~54岁、55~64岁女性(P﹤0.05);45~54岁女性宫颈癌患病率显著高于35~44岁、55~64岁女性(P﹤0.05)。结论:35~44岁是广东粤北瑶族地区农村妇女宫颈液基细胞学检查异常率和 CIN

  5. CORRELATION OF FINE NEEDLE ASPIRATION CYTOLOGY WITH BRONCHOALVEOLAR LAVAGE AND BRUSH SMEAR CYTOLOGY IN PULMONARY LESIONS

    Directory of Open Access Journals (Sweden)

    Surabhi

    2014-06-01

    Full Text Available BACKGROUND: Fine Needle Aspiration Cytology (FNAC, Bronchoalveolar Lavage (BAL and Brush Cytology are important diagnostic tools for evaluation of pulmonary lesions. Considering the limitations of these procedures correlation of these cytological techniques may help in improving accuracy and increasing the diagnostic yield. AIM: To determine the role of FNAC, Brush and BAL Cytology in pulmonary lesions and to correlate the efficacy of FNAC with Brush and BAL Cytology. MATERIALS AND METHODS: This prospective study was conducted on 78 patients with radiologically demonstrable pulmonary lesions in the department of pathology and Dr ML Chest Hospital, GSVM Medical College, Kanpur over a period from nov2011-july2013. The cases selected had to give consent for the procedure. Bronchoalveolar lavage, bronchial brush sample were taken and FNAC was done in the cases. RESULTS: In present study 78 cases of pulmonary lesions were subjected to FNAC and/ or Bronchoalveolar lavage and brush cytology. Among them 49 cases were selected in which all three procedures were done. The sensitivity and specificity for detecting lung tumors by FNAC was 96.29% and 95.45% which was more than that of BAL cytology (84.61%, 91.30% and Brush cytology (81.48%, 95.45%. FNA correlates with Brush and BAL cytology in 78.57% of malignant lesions. Among malignant lesions 84.61% of BAL and Brush cytology correlates with FNA for diagnosis of squamous cell carcinoma. FNA correlates with 66.67% of brush and 83.37% of BAL fluid cytology for diagnosis of adenocarcinoma. BAL and Brush both correlates with FNA in 83.33% cases of small cell carcinoma. Overall 85.71% of BAL and 82.14% of brush smear cytology correlates with FNA cytology for diagnosis of lung cancers. CONCLUSIONS: FNA, BAL and Brush cytology provide a high yield for evaluation of pulmonary lesions. BAL fluid and brush cytology correlated well with FNAC for diagnosis of lung cancers.

  6. Correlation of DNA Ploidy with Progression of Cervical Cancer

    Directory of Open Access Journals (Sweden)

    M. Singh

    2008-01-01

    Full Text Available The majority of squamous cell carcinomas of cervix are preceded by visible changes in the cervix, most often detected by cervical smear. As cervical cancer is preceded by long precancerous stages, identification of the high-risk population through detection of DNA ploidy may be of importance in effective management of this disease. Here we attempted to correlate aneuploid DNA patterns and their influence on biological behavior of flow-cytometry analysis of DNA ploidy which was carried out in cytologically diagnosed cases of mild (79, moderate (36, and severe (12 dysplasia, as well as “atypical squamous cells of unknown significance (ASCUS” (57 along with controls (69, in order to understand its importance in malignant progression of disease. Cytologically diagnosed dysplasias, which were employed for DNA ploidy studies, 39 mild, 28 moderate, and 11 severe dysplasia cases were found to be aneuploid. Out of the 69 control subjects, 6 cases showed aneuploidy pattern and the rest 63 subjects were diploid. An aneuploidy pattern was observed in 8 out of 57 cases of cytologically evaluated ASCUS. The results of the followup studies showed that aberrant DNA content reliably predicts the occurrence of squamous cell carcinoma in cervical smear. Flow cytometric analysis of DNA ploidy may provide a strategic diagnostic tool for early detection of carcinoma cervix. Therefore, it is a concept of an HPV screening with reflex cytology in combination with DNA flow cytometry to detect progressive lesions with the greatest possible sensitivity and specificity.

  7. Cervical Stenosis

    Science.gov (United States)

    ... Accumulation of pus in the uterus is called pyometra. Symptoms Before menopause, cervical stenosis may cause menstrual ... present but not cause symptoms. A hematometra or pyometra can cause pain or cause the uterus to ...

  8. Cervical spondylosis

    Science.gov (United States)

    ... Past neck injury (often several years before) Past spine surgery Ruptured or slipped disk Severe arthritis Small fractures ... Kshettry VR. Cervical spondylosis. In: Benzel EC, ed. Spine Surgery . 3rd ed. Philadelphia, PA: Elsevier Saunders; 2012:chap ...

  9. Cervical Cancer

    Science.gov (United States)

    ... Cervical cancer is caused by a virus called HPV. The virus spreads through sexual contact. Most women's bodies are able to fight HPV infection. But sometimes the virus leads to cancer. You're at higher risk ...

  10. Cervical Cancer

    Science.gov (United States)

    ... 162 KB) This information in Spanish (en español) Female reproductive system Select image to view larger Related ... D., FACS, Captain, U.S. Public Health Service Medical Director, National Breast and Cervical Cancer Early Detection Program, ...

  11. Prospects for controlling cervical cancer at the turn of the century

    Directory of Open Access Journals (Sweden)

    Franco Eduardo L

    2003-01-01

    Full Text Available Cervical cancer morbidity and mortality have decreased substantially during the last 50 years mostly due to success-ful organized or opportunistic screening with Pap cytology in high and middle income countries. In many low income countries Pap cytology screening is yet to be effectively implemented or has failed to reduce cervical cancer rates to an appreciable extent. The fact that infection with certain human papillomavirus (HPV types is now recognized as a necessary cause of this disease has led to new research fronts on prevention of cervical cancer. Testing for HPV DNA has shown great promise as a screening tool with better sensitivity but somewhat lower specificity than Pap cytology. In combination with the latter, HPV testing has the potential to improve the negative predictive value of cytology, thus allowing for increased testing intervals, which would lower program costs with acceptable safety. Advances in cytology processing and automation have also led to new screening approaches that are increasingly gaining acceptance in high and middle income countries. For low income countries, visual inspection with acetic acid has proven to be an effective alternative to conventional Pap cytology, especially in settings where no screening programs have been implemented. Concerning primary prevention of cervical cancer, recent research on the safety and efficacy of candidate prophylactic vaccines against HPV have shown very promising results with nearly 100% efficacy in preventing persistent infections and development of cervical cancer precursors. However, policy makers are strongly cautioned to avoid deferring decisions concerning the implementation of cervical cancer screening under the expectation that a successful vaccine could obviate the need for secondary prevention strategies.

  12. 宫颈癌机会性筛查的临床价值%Clinical Value of Cervical Cancer Opportunistic Screening

    Institute of Scientific and Technical Information of China (English)

    吴新容

    2012-01-01

    目的 分析研究宫颈癌机会性筛查的意义及临床价值.方法 以我院2007年11月至2010年11月在妇科门诊对前来就诊的患者进行宫颈癌机会性筛查研究.接受筛查对象均进行液基细胞学检查,细胞学异常行HPV结合阴道镜检查.并以患者进行活检组织病理学诊断作为宫颈癌的定性诊断.结果 2402例患者愿意接受筛查,顺应性为98.84%;液基细胞学阳性检出率为11.65%;阴道镜检查感染率为33.21%;病理活检阳性率为21.23%.将此次研究与2003 ~2005年的研究进行比较,存在明显差异(P<0.05).结论 通过对患者宫颈癌的机会性筛查,有效地提高了防治效果及群众对宫颈癌的认知度,对宫颈癌的早期诊治具有十分重要的意义,值得临床推广.%Objective To analyze the significance and clinical value of opportunistic screening in cervical cancer. Methods Patients in gynecologic clinic from Nov. 2007 to Nov. 2010 were given opportunistic screening of cervical cancer. All of them were performed liquid-based cytology examination. Those with abnormal cytology were given HPV combined with colposcopy examination. Pathological diagnosis of biopsy tissues were regarded as qualitative diagnosis of cervical cancer. Results 2402 patients were willing to take screening, the compliance was 98.84% ; liquid-based cytology detection rate was 11.65% ; colposcopy examination infection rate was 33.21%; pathologic biopsy positive rate was 21.23 %. Compared with the results of 2003 -2005,the difference was statistically significant,? <0. 05. Conclusion Opportunistic screening can effectively improve cognition degree of cervical cancer. It has very important significance to the early diagnosis and treatment of the disease,which is worth of spreading in clinic.

  13. Cervical cancer screening in Belgium and overscreening of adolescents.

    Science.gov (United States)

    Van Kerrebroeck, Helena; Makar, Amin

    2016-03-01

    There has been a marked decrease in the incidence of cervical cancer thanks to cytological screening with the Pap smear test. In Belgium, this screening is rather opportunistic. Over 39% of Belgian women between 25 and 64 years of age are never or only rarely screened by cytological tests. Moreover, there is an excess use of Pap smears because of women who rely on their yearly cervical smear and because many Pap smears are obtained from women beyond the target age range of 25 to 64 years. Sexually active adolescents are increasingly being recognized as a population distinct from adult women. They are at a high risk of acquiring the human papillomavirus (HPV), but most infections and cervical intraepithelial lesions caused by HPV are efficiently cleared by the immune system. We present a description of cervical cancer screening in Belgium using the database of the National Health Insurance Institute (RIZIV/INAMI) and the Belgian Health Care Knowledge Centre (KCE). We describe why elimination of Pap testing in the adolescent population reduces costs and harms without increasing cervical cancer rates. Expectant management, education on the risk factors for cervical cancer and HPV persistence, and HPV vaccination are very important in adolescents and young adults. PMID:25812038

  14. New Molecular Tools for Efficient Screening of Cervical Cancer

    Directory of Open Access Journals (Sweden)

    Magnus von Knebel Doeberitz

    2001-01-01

    Full Text Available Cytological screening using the Pap-smear led to a remarkable reduction of the mortality of cervical cancer. However, due to subjective test criteria it is hampered by poor inter- and intra-observer agreement. More reproducible assays are expected to improve the current screening and avoid unnecessary medical intervention and psychological distress for the affected women. Cervical cancer arises as consequence of persistent high risk papillomavirus (HR-HPV infections. Expression of two viral oncogenes, E6 and E7, in epithelial stem cells is required to initiate and maintain cervical carcinogenesis and results in significant overexpression of the cellular p16INK4a protein. Since this protein is not expressed in normal cervical squamous epithelia, screening for p16INK4a over-expressing cells allows to specifically identify dysplastic lesions, and significantly reduces the inter-observer disagreement of the conventional cytological or histological tests. Progression of preneoplastic lesions to invasive cancers is associated with extensive recombination of viral and cellular genomes which can be monitored by detection of papillomavirus oncogene transcripts (APOT assay derived from integrated viral genome copies. Detection of integrated type oncogene transcripts points to far advanced dysplasia or invasive cancers and thus represents a progression marker for cervical lesions. These new assays discussed here will help to improve current limitations in cervical cancer screening, diagnosis, and therapy control.

  15. Determinants of clearance of human papillomavirus infections in Colombian women with normal cytology: a population-based, 5-year follow-up study.

    NARCIS (Netherlands)

    Molano, M; Brule, den A Van; Plummer, M; Weiderpass, E; Posso, H; Arslan, A; Meijer, C.J.L.M.; Munoz, N.; Franceschi, S

    2003-01-01

    Little is known about the factors that influence clearance of human papillomavirus (HPV), the primary cause of cervical carcinoma. A total of 227 women cytologically normal and HPV positive at baseline were identified from a population-based cohort of 1,995 Bogota, Colombia, women aged 13-85 years f

  16. Co-infections associated with human immunodeficiency virus type 1 in pregnant women from southern Brazil: high rate of intraepithelial cervical lesions

    Directory of Open Access Journals (Sweden)

    Michele Tornatore

    2012-03-01

    Full Text Available Human immunodeficiency virus type 1 (HIV-positive pregnant women require specific prophylactic and therapeutic approaches. The efficacy of established approaches is further challenged by co-infection with other sexually transmitted diseases (STDs. The objective of this study was to determine the prevalence of co-infections in pregnant women infected with different HIV-1 subtypes and to relate these findings, together with additional demographic and clinical parameters, to maternal and infant outcomes. Blood samples from pregnant women were collected and tested for syphilis, hepatitis B virus (HBV and hepatitis C virus (HCV. Human papillomavirus (HPV diagnosis was evaluated by the presence of alterations in the cervical epithelium detected through a cytopathological exam. Medical charts provided patient data for the mothers and children. Statistical analyses were conducted with STATA 9.0. We found a prevalence of 10.8% for HCV, 2.3% for chronic HBV, 3.1% for syphilis and 40.8% for HPV. Of those co-infected with HPV, 52.9% presented high-grade intraepithelial lesions or in situ carcinoma. Prematurity, birth weight, Apgar 1' and 5' and Capurro scores were similar between co-infected and non-co-infected women. The presence of other STDs did not impact maternal and concept outcomes. More than half of the patients presenting cervical cytology abnormalities suggestive of HPV had high-grade squamous intraepithelial lesions or cervical cancer, evidencing an alarming rate of these lesions.

  17. CORRELATION OF CERVICAL DYSPLASIA WITH SEROLOGICAL AND MICROBIOLOGICAL TESTS FOR GENITAL INFECTIONS

    Directory of Open Access Journals (Sweden)

    Ishita

    2015-01-01

    Full Text Available AIMS : Prevalence of Sexually transmitted infections (STIs tends to be high which constitutes an important public health problem at present worldwide and in India, being associated with Human Immunodeficiency virus and predisposing to cervical dysplasia and cancer. There is a need to overcome the STI infections throughout the nation by cytological and microbiological screening. SETTING AND DESIGN : The present study was undertaken to investigate the association of Chlamydia trachomatis (CT, Herpes simplex virus 2 (HSV 2, Cytomegalovirus (CMV, Hepatitis B (HBV, Hepatitis C virus (HCV, Trichomonas vaginalis (TV and Candidiasis with cervical cytological changes in symptomatic female in West Bengal. METHOD S : Sera and vaginal samples from 431 syndromic female patients with cervico - vaginal discharge were tested for detection of STIs. The study period extended over two years. Concomitantly, study of cervical cytology on Papanicolaou stained smears was performed in all cases. ELISA was performed for detection of CT IgM, HSV2 IgM, CMV IgM, HBsAg and HCV IgM in sera. Candida spp. and TV were identified by culture of vaginal samples. Cytological findings were interpreted according to Bethesda 2001 classification. Statistical analysis was performed using MedCalc for Windows, version 13.3.1.0 (MedCalc Software, Ostend, Belgium. RESULTS: Out of 431 samples, majority of the patients belonged to the age group of 29 to 42 years, however the mean age of patients presenting with HSIL on PAP was 45 ± 7.93 years. CT, HSV2, TV and Candida spp. were associated with cervical cytological changes of ASCUS or worse. Age greater than 40 years and infection with CT emerged independent risk factors associated with cervical dysplasia on univariate and multivariate analysis with p <0.05 in both. CONCLUSION : C. trachomatis is a risk factor for cervical dysplasia. Early detection and treatment will prevent progression of STIs and development of cervical dysplasia and

  18. Limitations of widely used high-risk human papillomavirus laboratory-developed testing in cervical cancer screening

    Directory of Open Access Journals (Sweden)

    Naryshkin S

    2012-11-01

    Full Text Available Sonya Naryshkin,1 R Marshall Austin21Department of Pathology, Mercy Health System, Janesville, WI; 2Department of Pathology, Magee-Womens Hospital of University of Pittsburgh Medical Center, Pittsburgh, PA, USAObjective: To increase awareness of the limitations of high-risk human papillomavirus (hrHPV laboratory-developed testing (LDT widely used in US cervical cancer screening.Methods and results: A young woman in her 30s was diagnosed and treated for stage 1B1 cervical squamous cell carcinoma in which HPV 16 DNA was detected using polymerase chain reaction testing. Both 1 month before and 42 months before cervical cancer diagnosis, the patient had highly abnormal cytology findings; however, residual SurePath™ (Becton, Dickson and Company, Franklin Lakes, NJ vial fluid yielded negative Hybrid Capture 2 (HC2; Qiagen NV, Hilden, Germany hrHPV LDT results from each of the two specimens. This prompted questions to be asked concerning the performance characteristics of hrHPV LDT. A review of the available data indicates that (1 purification of DNA from SurePath specimens requires complex sample preparation due to formaldehyde crosslinking of proteins and nucleic acids, (2 HC2–SurePath hrHPV testing had not been Food and Drug Administration-approved after multiple premarket approval submissions, (3 detectible hrHPV DNA in the SurePath vial decreases over time, and (4 US laboratories performing HC2–SurePath hrHPV LDT testing are not using a standardized manufacturer-endorsed procedure.Conclusion: Recently updated cervical screening guidelines in the US recommend against the use of hrHPV LDT in cervical screening, including widely used HC2 testing from the SurePath vial. The manufacturer recently issued a technical bulletin specifically warning that use of SurePath samples with the HC2 hrHPV test may provide false negative results and potentially compromise patient safety. Co-collection using a Food and Drug Administration-approved hrHPV test

  19. Determining the interobserver reproducibility of Pap smears in the diagnosis of epithelial cell abnormalities

    Directory of Open Access Journals (Sweden)

    Izadi-Mood N

    2011-07-01

    Full Text Available "n 800x600 Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif";} Background: Cancer of uterine cervix is the second cause of death in women in the world and the most common cause in developing countries. Because the majority of women with invasive cervical cancer of the uterine have not previously undergone screening, many clinicians assume that Pap smear has a high degree of accuracy; but problems such as false positive and false negative interpretations, as well as interobserver variability have questioned its validity."n"nMethods : We retrieved 162 positive cervical smears that had been originally interpreted as ASC-US, ASC-H, LSIL, HSIL, SCC, AGC and adenocarcinoma from the cytology archives of Women's Hospital in Tehran, Iran. The slides were rescreened by an experienced pathologist and reclassified in the mentioned categories. All the 162 slides were reviewed by three more pathologists in a blind study using interpretative criteria utilized in their daily routine to evaluate interobserver reproducibility. To increase the level of interobserver agreement, the diagnostic categories were reduced to squamous Vs. glandular abnormalities and invasive (SCC and adenocarcinoma Vs. non-invasive abnormalities."n"nResults : The results obtained in this study indicated slight interobserver agreement (k=0.26. The most reproducible category was the invasive category (SCC in addition to adenocarcinoma and the least agreement was seen for HSIL (k=0.19. "n"nConclusion: This study showed that reproducibility of cytological interpretation of conventional Pap smears

  20. The potential therapeutic targets for cervical cancer

    OpenAIRE

    L Priyanka Dwarampudi; Gowthamarajan, K.; Shanmugam, R; Madhuri, K.; Nilani, P.; M N Satish Kumar

    2013-01-01

    In case of invasive cervical carcinoma several molecular events were reported and these molecular events resulting in multiple genetic abnormalities. In order to control these tumors multiple molecular therapeutic targets are needed with different molecular mechanisms. Unfortunately, these molecular targets were in early stages of development. Because of less degree of success of conventional therapeutics for late stages of cervical cancer and lowering of prognosis of patients there is an inc...

  1. Cytological evaluation and prediction of progression of acute erosive ulcered lesions of upper parts of gastrointestinal tract in acute and early periods of cerebrospinal trauma

    Directory of Open Access Journals (Sweden)

    Norkin I.A.

    2010-09-01

    Full Text Available 80 cytological preparations derived by fibrogastroduodenoscopy from 20 patients with cerebrospinal trauma at cervical part level served as the research subject. Dynamics of the progression of acute erosive ulcered lesions of mucous membrane of the upper parts of the gastrointestinal tract was studied on the basis of the cytological analyses of mucous membrane biopsy materials. In the course of our work we used endoscopic (fibrogastroduodenoscopy and cytological research methods. Cytological analyses of mucous membrane biopsy materials were carried out on the 7th, 14th, 21st and 28th day. Biopsy material cellular composition was evaluated on the grounds of the calculation of neutrophilic leukocytes and epithelial cells with the use of an immersion objective. In so doing we registered neutrophilic leukocyte number for 100 cells and determined neutrophilic and epithelial index. Monitoring of neutrophilic leukocyte number enables to determine presence or absence of inflammatory changes in stomach mucous membrane and duodenum in different periods of cerebrospinal trauma

  2. Fine needle aspiration cytology findings in cases diagnosed as oropharyngeal tularemia lymphadenitis (Erratum was published in Vol 24 Number 1, 2008

    Directory of Open Access Journals (Sweden)

    Banu DOĞAN GÜN

    2007-01-01

    Full Text Available Francisella tularensis is a gram-negative coccobacilus that causes zoonotic disease tularemia. Histopathological examination of lymph node biopsy in tularemia reveals suppurative granulomatous inflammation potentially associated caseous necrosis. Diagnosis is mainly made on the evidence of elevated agglutinating antibodies against F. Tularensis. In this study we aimed to evaluate the cytological features of ulceroglandular tularemia cases and to demonstrate the role of fine needle aspiration cytology in the diagnosis of tularemia.Fine needle aspiration cytology findings of six cervical lymphadenopaties that had established diagnoses of tularemia both clinically and serologically, were evaluated and the cytomorphological features were described.All of the cases revealed suppurative inflammation and some caseous necrosis and in four cases epithelioid histiocytes and multinuclear giant cells were observed additionally.The differential diagnosis of tularemia principally from tuberculosis and other types of bacterial lymphadenitis was made and the place of fine needle aspiration cytology among other diagnostic laboratory tests for tularemia was evaluated.

  3. The role and application of exfoliative cytology in the diagnosis of oral mucosa pathology - contemporary knowledge with review of the literature.

    Science.gov (United States)

    Kazanowska, Krzysztofa; Hałoń, Agnieszka; Radwan-Oczko, Małgorzata

    2014-01-01

    On the basis of the current available literature, the authors have presented a short description of cytological examination and its application in the oral mucosa disease diagnostic process. Furthermore, the advantages and disadvantages of this method are described. The available diagnostic tools used for oral smears were reviewed as well as more and more often available methods which aim at making the diagnosis process more accurate and more favorable for patients. Oral cytology analysis may, in the near future, be a very useful examination for patients in terms of diagnostics and monitoring, not only during the treatment but also afterwards. The authors would like to demonstrate what a beneficial tool this cytological examination could be as a fast and cheap cancer prophylactic test. This opinion is based on the fact that this cytological method has significantly improved the detection of uterine cervical cancer during a gynecological examination since the introduction of the Papanicolau technique in the 40s.

  4. Addition of high-risk HPV testing improves the current guidelines on follow-up after treatment for cervical intraepithelial neoplasia

    NARCIS (Netherlands)

    M.A.E. Nobbenhuis (Marielle); C.J.L.M. Meijer (Chris); A.J.C. van den Brule; L. Rozendaal; F. Voorhorst (Feja); E.K.J. Risse (Elle); R.H.M. Verheijen (René); T.J.M. Helmerhorst (Theo)

    2001-01-01

    textabstractWe assessed a possible role for high-risk human papillomavirus (HPV) testing in the policy after treatment for cervical intraepithelial neoplasia (CIN) 2 or 3 (moderate to severe dysplasia). According to the Dutch guidelines follow-up after treatment consists of cervical cytology at 6, 1

  5. Incidence of cervical dysplasia and cervical cancer in women living with HIV in Denmark

    DEFF Research Database (Denmark)

    Thorsteinsson, K; Ladelund, S; Jensen-Fangel, S;

    2016-01-01

    OBJECTIVES: Women living with HIV (WLWH) are reportedly at increased risk of invasive cervical cancer (ICC). A recent publication found that WLWH in Denmark attend the national ICC screening programme less often than women in the general population. We aimed to estimate the incidence of cervical...... performed to include prior screening outcome, screening intensity and treatment of CIN/ICC in the interpretation of results. RESULTS: We followed 1140 WLWH and 17 046 controls with no prior history of ICC or hysterectomy for 9491 and 156 865 person-years, respectively. Compared with controls, the overall...... in both groups were adherent to the national ICC screening programme and had a normal baseline cytology, incidences of CIN and ICC were comparable. CONCLUSIONS: Overall, WLWH developed more cervical disease than controls. Yet, in WLWH and controls adherent to the national ICC screening programme...

  6. Initiation of an anal cancer screening in HIV+MSM: results of cytology, biopsy and determination of risk factors

    Directory of Open Access Journals (Sweden)

    A Libois

    2012-11-01

    Full Text Available Incidence of anal cancer is increasing and risk of anal cancer is higher in MSM, especially if they are HIV+. European guidelines for treatment of HIV-infected adults recommend anal cancer screening by digital rectal exam±Pap test with anuscopy if Pap test is abnormal. A systematic anal cancer screening in HIV+MSM with anal cytology (Pap smears was established in June 2011 in our reference centre in Brussels. If anal cytology was abnormal, high-resolution anuscopy (HRA with biopsy was performed. 353 MSM HIV+were screened by anal smears between June 2011 and May 2012. 90% were Caucasians, median age was 44.5 years, 83% were on HAART and 74% had an undetectable viral load, median CD4 was 632/µl and 33% had a nadir CD4<200. Thirty-three (9.3% were excluded because of poor quality. Cytology was abnormal in 46% of the 320 remaining patients: high-grade squamous intraepithelial lesion (HSIL 3%, low-grade squamous intraepithelial lesion (LSIL 24%, atypical squamous cells of undetermined significance (ASC-US 16%, and atypical squamous cells / cannot rule out a high-grade lesion (ASC-H 3%. Viral load (VL was more frequently undetectable (82% vs 64%, p=0.0003 and median duration of HAART was longer (111 vs 61 months, p=0.0145 in patients with normal cytology. 80 HRA with biopsies have been performed. 12.5% were normal, 44% showed anal intraepithelial neoplasia (AIN 1, 24% AIN 2 and 19% AIN 3. For this analysis, high-grade AIN (2 and 3 were put together (AIN 2+. Among patients with AIN 2+(n=33, cytology had showed 8 (24% ASC-US, 3 (9% ASC-H, 19 (57% LSIL, 3 (9% HSIL. When patients with normal cytology or normal biopsy and patients with AIN 2+were compared, the only significant risk factor found for AIN 2+was a nadir CD4<100/µl (32% of the patients with AIN 2+vs 14% in patients with normal smear, p=0.0073. Anal precancerous lesions are frequent and at different stages. Among 46% abnormal cytology, 87% had abnormal biopsy including half AIN 2+.Cytology

  7. Frequency and risk factors of cervical human papilloma virus infection in women in Montenegro

    Directory of Open Access Journals (Sweden)

    Mijović Gordana

    2014-01-01

    Full Text Available Cervical human papilloma virus (HPV infection among women from 17 to 62 years of age in Montenegro was studied using in situ hybridization and cytological testing. Cervical HPV infection was diagnosed in 44.3% of women. The most common HPV genotype was 31/33/51 found in 56.9%. HPV positive samples for types 16/18 were identified in 41% HPV positive samples. The results showed that 57% and 45% of women who had had their first sexual intercourse before and after the age of 18, respectively, had cervical HPV infection. Cytological findings of Papanicolaou (Pap test class III were significantly more common in women smokers. Cervical HPV infection among women in Montenegro is most commonly caused by HPV genotypes for which a prophylactic vaccine is available, or by phylogenetically related types, thus offering the opportunity of using vaccines to reduce the incidence of HPV infection.

  8. Cytologic diagnosis of pulmonary lesions.

    Science.gov (United States)

    Rossi, Esther Diana; Mulè, Antonino; Maggiore, Claudia; Miraglia, Antonella; Lauriola, Libero; Vecchio, Fabio Maria; Fadda, Guido

    2004-01-01

    The major types of cytologic preparations used in most laboratories to detect the lesions of the lower respiratory tract (LRT) are examined. These methods include sputum, bronchial washing, bronchial brushing, bronchoalveolar lavage (BAL) and fine-needle aspiration biopsy (FNAB). Sputum represents the simplest and most cost-effective sampling method even though fiberoptic bronchoscopy and radiologic guided FNAB are superseding it as the first diagnostic choice in most cases. There are advantages and disadvantages associated with each technique:bronchial brushing and FNABs tend to preserve both the cellular details and their architectural arrangement whereas sputum and bronchial washing often cause a variable degree of cellular degeneration and fragmentation. As a result, most pulmonary lesions may be detected and correctly diagnosed if multiple techniques are used to acquire diagnostic material. CT-guided FNAB represents the most effective method to achieve a correct diagnosis in pulmonary tumors. PMID:15852720

  9. Bacteriological techniques compliment the clinical and cytological diagnosis of tuberculosis in human immuno deficiency virus infected persons

    Directory of Open Access Journals (Sweden)

    Srikantam A

    2006-01-01

    Full Text Available Human immunodeficiency virus (HIV and M. tuberculosis are two intracellular pathogens that interact with each other at both clinical and cellular levels. A known HIV positive case, presentingwith vague pulmonary symptoms followed by bilateral cervical lymphadenopathy, is reported here. The condition was treated as asthma, based on lung symptoms. Cytology revealed acute purulent lymphadenitis. Only Z. N. staining and culture of sputum and lymphnode aspirate could diagnose tuberculosis. Patient responded well to the standard anti tuberculosis treatment.

  10. Cytologic patterns of lymph node diseases in Hawassa University Referral Hospital, Southern Ethiopia

    Institute of Scientific and Technical Information of China (English)

    Gemechu Ameya Buli; FekadeYerakly Lukas

    2015-01-01

    Objective: To assess the cytological patterns of lymph node diseases in patient attended Hawassa University Referral Hospital, Southern Ethiopia. Methods: A five years retrospective descriptive study design was conducted on fine needle aspiration cytology reports of patients referred to pathology laboratory of Hawassa University Referral Hospital from September, 2009 to September, 2014. Results: A total of 1 067 lymph nodes were aspirated in the study period. Cervical lymphadenopathy was the most frequent (48.82%) followed by submandibular (22.77%) lymph nodes. The age group of 11-20 years was the most affected age group while age group above 60 with less frequency. Tuberculosis lymphadenitis was the highest (48.82%), chronic non-specific lymphadenitis (20.33%), reactive (16.21%), pyogenic abscess (5.99%) and the rest were malignancies. Conclusions: Lymphadenopathy can be associated with a wide range of disorders. However tuberculosis lymphadenitis is the most common cause of enlarged lymph node in the study area.

  11. Application of cellular DNA ploid analysis system in clinical screening of cervical cancer%细胞DNA倍体分析系统在临床宫颈癌筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    黄熙理; 邱峰; 洪丽美; 陈智颖

    2015-01-01

    Objective:To explore the application of cellular DNA ploid analysis system in clinical screening of cervical cancer. Methods:The cervix cells were checked through cellular DNA ploid analysis system and liquid thin-layer cytology test(TCT). Results:A total of 693 cases had cervical cytology and (or) DNA ploid abnormalities;270 cases had positive liquid cytology and the positive rate was 4.5%.668 cases were detected abnormality through DNA ploid analysis system and the positive detection rate was 11.1%.With three or more DNA heteroploid appearing as the standard biopsy,the sensitivity of cervical lesions was 78.9%;the specificity was 43.27%;the positive expected value was 63.13%;the negative expected value was 62.5%.With liquid cytology test above LSIL as the standard biopsy,the sensitivity of cervical lesions was 43.75%;the specificity was 79.81%;the positive expected value was 72.72% ;the negative expected value was 53.55% .Conclusion:Cervical cellular DNA ploid analysis system had the objectivity and high sensitivity,which combined liquid thin-layer cytology test could reduce the misdiagnose and misdiagnosis and improve the specificity of the check.%目的:探讨细胞DNA倍体分析系统在临床宫颈癌筛查中的应用。方法:对宫颈细胞行宫颈DNA倍体分析及液基薄层细胞学检查(TCT)。结果:共检出宫颈细胞学和(或)DNA倍体异常693例,液基细胞学阳性270例,阳性率4.5%。DNA倍体分析系统检出668例异常,阳性检出率11.1%。以≥3个DNA异倍体出现作为活检标准,宫颈病变的敏感性78.9%,特异性43.27%,阳性预期值63.13%,阴性预期值62.5%。以液基细胞学检查 LSIL 以上作为活检标准,宫颈病变的敏感性43.75%,特异性79.81%,阳性预期值72.72%,阴性预期值53.55%。结论:宫颈细胞DNA倍体分析系统具有客观性及较高的敏感性,与宫颈液基细胞学检查联合应用,可减少误诊、漏诊,提高检查的特异性。

  12. A CLINICOPATHOLOGICAL STUDY OF CERVICAL LYMPHADENOPATHY

    Directory of Open Access Journals (Sweden)

    Abhishek

    2015-03-01

    Full Text Available BACKGROUND AND OBJECTIVE: Cervical lymphadenopathy is the most common site of peripheral lymphadenopathy and is frequently encountered in otorhinolaryngology practice. Assessment and predicting its clinical behavior is not an easy task. Fine needle aspiration cytology (FNAC is being routin ely adopted as a special technique to diagnose the cause of lymphadenopathy. This study was undertaken to identify the clinic - demographic parameters in distribution of cases of cervical lymphadenopathy. FNAC was evaluated as a diagnostic tool by corroborat ing its results with histopathological examination of the excised lymph nodes. MATERIAL AND METHODS: This study was carried out at Hi - Tech Medical College and Hospital, Bhubaneswar, on 100 patients of cervical lymphadenopathy, over a period of two years. A long with detailed history, meticulous clinical examinations and investigations were performed. In all cases the diagnosis provided by FNAC and histopathology examination of the excised lymph node were correlated. RESULTS: Tuberculosis (45% was the most c ommon cause of cervical lymphadenopathy, followed by reactive (26% and metastatic secondaries (21% and lymphoma (8%. Maximum presentation was in 3 rd decade (22% and bilateral involvement was seen in 20% cases. Posterior triangle was the most common sit e involved (45%. Overall diagnostic accuracy of FNAC was 92%. CONCLUSION: Most common cause of cervical lymphadenopathy are tuberculosis, reactive lymphadenitis and metastatic secondaries. FNAC is a cheap, quick, readily available and dependable diagnostic modality and can be used as a first line investigatory tool in outdoor departments.

  13. B-Flow Twinkling Sign in Preoperative Evaluation of Cervical Lymph Nodes in Patients with Papillary Thyroid Carcinoma

    Directory of Open Access Journals (Sweden)

    Giuseppina Napolitano

    2013-01-01

    Full Text Available Papillary thyroid cancer (PTC is the most common histologic type of differentiated thyroid cancer. The first site of metastasis is the cervical lymph nodes (LNs. The ultrasonography (US is the best diagnostic method for the detection of cervical metastatic LNs. We use a new technique, B-flow imaging (BFI, recently used for evaluation of thyroid nodules, to estimate the presence of BFI twinkling signs (BFI-TS, within metastatic LNs in patients with PTC. Two hundred and fifty-two patients with known PTC were examined for preoperative evaluation with conventional US and BFI. Only 83 with at least one metastatic LN were included. All patients included underwent surgery; the final diagnosis was based on the results of histology. The following LN characteristics were evaluated: shape, abnormal echogenicity, absent hilum, calcifications, cystic appearance, peripheral vascularization, and BFI-TS. A total of 604 LNs were analyzed. Of these, 298 were metastatic, according to histopathology. The BFI-TS showed high values ​​of specificity (99.7% and sensitivity (80.9%. The combination of each conventional US sign with the BF-TS increases the specificity. Our findings suggest that BFI can be helpful in the selection of suspicious neck LNs that should be examined at cytologic examination for accurate preoperative staging and individual therapy selection.

  14. The value of electromyography in differentiating intramedullary tumor from inflammatory demyelinating disease of cervical region

    Institute of Scientific and Technical Information of China (English)

    王红芬

    2014-01-01

    Objective To investigate the value of needle electromyography(EMG)in differentiating intramedullary tumor from inflammatory demyelinating disease of cervical region.Methods Patients hospitalized in the Chinese PLA General Hospital from March 2008 to June 2013 with abnormalities on MRI of cervical vertabra and preliminary diagnosed as intramedullary tumor or inflammatory demyelinating disease of cervical region were enrolled in the

  15. Os Odontoideum: Rare Cervical Lesion

    Directory of Open Access Journals (Sweden)

    Kristie A Robson

    2011-05-01

    Full Text Available We report the case of a 22-year-old Marine who presented to the emergency department, after a martial arts exercise, with transient weakness and numbness in all extremities. Computed tomography cervical spine radiographs revealed os odontoideum. Lateral flexion–extension radiographs identified atlanto-axillary instability. This abnormality is rare and can be career ending for military members who do not undergo surgical fusion. [West J Emerg Med. 2011;12(4:520–522.

  16. Avaliação da sensibilidade e especificidade dos exames citopatológico e colposcópico em relação ao exame histológico na identificação de lesões intra-epiteliais cervicais Sensibility and specificity of cytology and colposcopy exams with the histological evaluation of cervical intraepithelial lesions

    Directory of Open Access Journals (Sweden)

    FELIPE FRANCISCO BONDAN TUON

    2002-06-01

    Maternidade Santa Brígida de Curitiba, PR, were selected. Those patients were sent to colposcopy under the following criteria: 1 previous abnormal cytopathological exams, 2 Indicative clinical data or, 3 Suspected lesions on gynecological exam. The statistical significance analysis of the results was done using the chi-square test. Sensibility, specificity, positive predictive value (PPV and negative predictive value (NPV were also determined.RESULTS: The patients' mean age was 30.2 (±10.9. Cytopathological capability of identifying lesions was 50% when compared to histology. Its specificity was 77%, the sensibility 41%, the PPV 74%, and the NPV 45%. Colposcopy capability of identifying lesions was 50%. Its sensibility, specificity, PPV and NPV were 96%, 19%, 65% and 75% respectively. The two methods associated were capable of identifying 63% of the lesions.CONCLUSIONS: Cytopathology was a high specificity exam, while colposcopy from those selected patients had a high sensibility. Colposcopy association with cytopathological screening, on those selected patients, significantly raises the diagnostic accuracy of cervical cancer precursor lesions.

  17. Adenomatatoid tumor: Cytological diagnosis of two cases

    Directory of Open Access Journals (Sweden)

    Kalyani R

    2009-01-01

    Full Text Available Adenomatoid tumor is a benign neoplasm of mesothelial cell origin that occurs in both male and female genital tracts. Fine needle aspiration cytology has an important role in the preoperative diagnosis of the male genital adenomatoid tumor and is a rapid, reliable, conclusive, and cost-effective diagnostic tool that can be used to take appropriate surgical decisions. Pathologists should be aware of the cytological features of such lesions so as to avoid diagnostic pitfalls. We present here two cases, one in the testis and another in the epididymis in a 35 year-old and a 30 year-old male respectively, which were diagnosed by fine needle aspiration cytology and later confirmed by histopathology. We present the cytological features and histopathological correlation of these cases.

  18. Low adherence to cervical cancer screening after subtotal hysterectomy

    DEFF Research Database (Denmark)

    Andersen, Lea Laird; Møller, Lars Mikael Alling; Gimbel, Helga Margrethe

    2015-01-01

    INTRODUCTION: A reason for not recommending subtotal hysterectomy is the risk of cervical pathology. We aimed to evaluate cervical cancer screening and to describe cervical pathology after subtotal and total hysterectomy for benign indications. METHODS: Data regarding adherence to screening.......7% were not screened. We found a minimum of one abnormal test in 28 (10.8%) after subtotal hysterectomy and one after total hysterectomy. No cervical cancers were found. CONCLUSIONS: Adherence to cervical cancer screening after subtotal hysterectomy in a Danish population is suboptimal and some patients...... have unnecessary tests performed after total hysterectomy. Clarification of the use of cervical/vaginal smears after hysterectomy is needed to identify women at risk of cervical dysplasia or cancer. FUNDING: Research Foundation of Region Zealand, University of Southern Denmark, Nykøbing Falster...

  19. Intelligent Screening Systems for Cervical Cancer

    Directory of Open Access Journals (Sweden)

    Yessi Jusman

    2014-01-01

    Full Text Available Advent of medical image digitalization leads to image processing and computer-aided diagnosis systems in numerous clinical applications. These technologies could be used to automatically diagnose patient or serve as second opinion to pathologists. This paper briefly reviews cervical screening techniques, advantages, and disadvantages. The digital data of the screening techniques are used as data for the computer screening system as replaced in the expert analysis. Four stages of the computer system are enhancement, features extraction, feature selection, and classification reviewed in detail. The computer system based on cytology data and electromagnetic spectra data achieved better accuracy than other data.

  20. TTV and HPV co-infection in cervical smears of patients with cervical lesions

    Directory of Open Access Journals (Sweden)

    Tachezy Ruth

    2009-07-01

    Full Text Available Abstract Background The female lower genital tract is a gateway for pathogens entering the host through the mucous membrane. One of the prevalent human viruses is Torque teno virus (TTV. The major reported routes of TTV transmission are fecal-oral and parenteral. Furthermore, other modes of transmission, e.g. sexual contact, are suggested. To investigate the sexual route of TTV transmission, cervical smears of healthy women and those with cervical lesions were screened for the presence of TTV DNA. Methods TTV DNA was studied in cervical smears of 95 patients with cervical lesions and 55 healthy women. Paired serum samples were available from 55 and 42 women, respectively. All healthy women had normal cytology while 44 patients had histologically confirmed low-grade lesion (LGL and 51 high-grade lesion (HGL. TTV DNA was detected with primers specific for the non-coding region. In 40 paired cervical smears and serum samples, the phylogenetic group of TTV isolates was determined. The presence of HPV DNA in cervical smears was detected by means of PCR with MY09/11 primers. Results The prevalence of TTV DNA in cervical smears of healthy women was 52.7% and was comparable with that in paired serum samples (50%. Symptomatic women had significantly higher prevalence of TTV DNA in cervical smears (74.7% than healthy controls. The TTV DNA prevalence in patient serum samples was 51%. The phylogenetic groups of TTV serum isolates were concordant with those of TTV from cervical smears of the same subjects. In cervical smears, a wider variety of TTV isolates was found. The viral loads in cervical smears were 10 to 1000 times as high as in sera. The HPV-positive study subjects had significantly higher TTV DNA prevalence than HPV negatives. The prevalence of TTV was not associated with disease severity. Conclusion High prevalence of TTV in cervical smears suggests that sexual transmission is another mode of expansion of TTV infection among the population. The

  1. COMPARATIVE STUDY OF EVALUATION OF SENSITIVITY AND SPECIFICITY OF CYTOLOGY AND COLPOSCOPY FOR DETECTION OF PRECANCEROUS LESIONS OF CERVIX

    Directory of Open Access Journals (Sweden)

    Sharma

    2013-12-01

    Full Text Available ABSTRACT : BACKGROUND : Cervical cancer is the second most common cancer among women globally and a leading cause of death due to cancer among women. If diagnosed earlier, it has a high cure rate but advanced disease is frequently incurable with v ery unpleasant consequences. MATERIAL AND METHODS : This was a cross sectional study done on a group of 50 women. A detailed clinical history of each patient was taken. The patients were made to lie in dorsal lithotomy position and per speculum examination was done. After evaluating the cervix, Paps smear was taken for cytology with Ayers spatula from ectocervix after rotating at 360 degree and spread on 2 glass slides to prepare thin films and kept in koplin jar and sent for cytology reporting. The patients were then subjected to colposcopy and punch biopsy. RESULTS : On comparison with biopsy cervix as gold standard for detection of CIN, cytology had shown Sensitivity - 57.14%, Specificity - 88.88%, NPV - 84.21%, PPV - 66.66%, whereas colposcopy had shown Sen sitivity - 80%, Specificity - 80%, NPV - 94.11%, PPV - 50%. CONCLUSION : In developing countries like India, cytology, a low cost and easily accessible test, is the most logical screening modality although it has very low sensitivity but detection rates coul d be further improved using liquid cytology and use of endocervicalcytobrush. Colposcopy with high sensitivity is although an important adjunctive screening modality but requires expertise and training for interpretation and is costly

  2. Investigation of Trichomonas Vaginalis by Parasitological and Cytological Methods in Patients Admitted to Gynecology Outpatient Clinics in a University Hospital

    Directory of Open Access Journals (Sweden)

    Özlem Aycan-Kaya

    2015-11-01

    Full Text Available Aim: In this study, it was aimed to compare parasitological and cytological diagnostic methods to detect T. Vaginalis in samples taken from posterior fornix of vagina. Methods: In this cross-sectional study, study population (n=104 were selected from women applying to gynecology outpatient clinic of Mustafa Kemal University Hospital with different complaints between March- August 2013. The presence of T. vaginalis was investigated by parasitological and cytological diagnostic methods. While direct microscopy, giemsa staining and culture in Cysteine-Peptone-Liver-Maltose medium (CPLM were performed for parasitological diagnosis of T. vaginalis in samples taken from posterior fornix of vagina, cervical smear stained by Papanicolaou (PAPS was used for cytological diagnosis. Results: T. vaginalis was detected in 12 (11.5%, 12 (11.5%, 14 (%13.4 of materials by direct microscopy, giemsa staining and culture, respectively. T. vaginalis was detected only 5 (4.8% of materials by cytology. This difference was statistically significant (p<0.05. Conclusion: Our study results showed that parasitological methods are more sensitive than cytological methods in diagnosis of T. vaginalis. Therefore, T. vaginalis should be screened in women with non-specific symptoms in under developed countries where lack of periodic examination and polygamy were seen.

  3. Touch imprint cytology of prostate core needle biopsy specimens: A useful method for immediate reporting of prostate cancer

    Directory of Open Access Journals (Sweden)

    Berna Aytac

    2012-01-01

    Conclusions: TIC smears can provide an immediate and reliable cytological diagnosis of prostate carcinoma. It may clearly help the rapid detection of carcinoma, particularly in highly suspected cases that had negative routine biopsy results for malignancy with abnormal serum prostate specific antigen (PSA levels and atypical digital rectal examination.

  4. Case Report: Liquid-based cytology aids in primary fallopian tube cancer diagnosis

    Institute of Scientific and Technical Information of China (English)

    Zhuo REN; Yun-ping ZHANG; Hui-xia YANG; Li-rong ZHU

    2009-01-01

    Primary fallopian tube carcinoma (PFTC) is a rare malignant carcinoma among all genital tract malignancies. It occurs most commonly in postmenopausal women and is similar to ovarian malignancy historically and clinically. Because of its insidious onset and silent course, the diagnosis is made usually postoperatively. Liquid-based cytology (LBC) is a type of method for cervical cancer screening, but sometimes it may aid in making PFTC diagnosis. We report a 47-year-old woman with PFTC, whose diagnosis was made with the aid of LBC.

  5. Role of scrape cytology in ovarian neoplasms

    Directory of Open Access Journals (Sweden)

    Rao Shalinee

    2009-01-01

    Full Text Available Aim: The present study was done to evaluate the role of scrape cytology in the diagnosis of ovarian neoplasm and its utilization for teaching pathology residents. Materials and Methods: This was a prospective study on 50 solid/solid-cystic ovarian neoplasms sent in 10% buffered formalin. Scrapings obtained from the fresh cut surface of tumors were smeared uniformly on to glass slides, immediately fixed in 95% ethyl alcohol and stained with hematoxylin and eosin stain. Results: The overall diagnostic accuracy of scrape cytology has been satisfactory with 92% of cases correlating with the final diagnosis. Characteristic cytological pattern was noted in various types of surface epithelial, sex cord stromal and germ cell tumors. The technique had limited value in mucinous tumors to distinguish borderline cases from invasive carcinoma. Two mucinous carcinomas were diagnosed as borderline mucinous tumor and two endometrioid carcinomas were misinterpreted as cystadenocarcinoma on scrape cytology. Formalin did not interfere or produce any remarkable changes in cytomorphology. Conclusions: Scrape cytology is a simple, rapid, accurate, inexpensive adjunctive cytodiagnostic technique and its routine utilization in ovarian lesions could aid in expanding the cytological knowledge of ovarian neoplasms.

  6. Cervical Cancer Screening after Perimenopause: How Is Human Papillomavirus Test Performed?

    Science.gov (United States)

    2016-01-01

    Cervical cancer is the third most prevalent cancer in women around the world. Recently in Korea, the incidence of cervical cancer has decreased, but in all stages of cervical intraepithelial neoplasia (CIN), CIN has shown a 91% increase from 1999 to 2008. Persistent human papillomavirus (HPV) infection has been found to be the main cause of cervical cancer. HPV types 16 and 18 have been found in 70% of cervical cancer patients around the world. Cervical cancer screening such as cytology has limitations in terms of sensitivity and specificity. A discussion about the need for the HPV test is becoming active in order to compensate for the limitation of cytology. After the role of HPV in cervical cancer was identified, the importance of HPV detection test as a screening was emphasized. Several tests have been developed and each test has its own advantages and disadvantages, and new test method to overcome the disadvantages is still being developed. Today's guidelines and tests are those you would choose from among the large number of cervical cancer screening guidelines and tests, based on the consideration that the selected guidelines and the test are effective.

  7. Cervical Cancer Screening after Perimenopause: How Is Human Papillomavirus Test Performed?

    Science.gov (United States)

    Chung, Soo-Ho

    2016-08-01

    Cervical cancer is the third most prevalent cancer in women around the world. Recently in Korea, the incidence of cervical cancer has decreased, but in all stages of cervical intraepithelial neoplasia (CIN), CIN has shown a 91% increase from 1999 to 2008. Persistent human papillomavirus (HPV) infection has been found to be the main cause of cervical cancer. HPV types 16 and 18 have been found in 70% of cervical cancer patients around the world. Cervical cancer screening such as cytology has limitations in terms of sensitivity and specificity. A discussion about the need for the HPV test is becoming active in order to compensate for the limitation of cytology. After the role of HPV in cervical cancer was identified, the importance of HPV detection test as a screening was emphasized. Several tests have been developed and each test has its own advantages and disadvantages, and new test method to overcome the disadvantages is still being developed. Today's guidelines and tests are those you would choose from among the large number of cervical cancer screening guidelines and tests, based on the consideration that the selected guidelines and the test are effective. PMID:27617239

  8. Risk for cervical intraepithelial neoplasia grade 3 or worse in relation to smoking among women with persistent human papillomavirus infection

    DEFF Research Database (Denmark)

    Jensen, Kirsten Egebjerg; Schmiedel, Sven; Frederiksen, Kirsten;

    2012-01-01

    , and subsequently cervical cells were obtained for HPV DNA testing. Women with high-risk HPV infection and no prevalent cervical disease at baseline (n=1,353) were followed through the Pathology Data Bank for cervical lesions for up to 13 years. Separate analyses of women with persistent high-risk HPV infection.......05-3.22, adjusted for length of schooling, parity and HPV type at baseline). The average number of cervical cytology screening tests per year during follow-up did not explain the differences in risk in relation to smoking (p=0.4). CONCLUSIONS: Smoking is associated with an increased risk for subsequent high...

  9. Extraordinary incidence of cervical ribs indicates vulnerable condition in Late Pleistocene mammoths

    NARCIS (Netherlands)

    Reumer, J.W.F.; ten Broek, C.M.A.; Galis, F.

    2014-01-01

    The number of cervical vertebrae in mammals is highly conserved at seven. We have shown that changes of this number are selected against due to a coupling with major congenital abnormalities (pleiotropic effects). Here we show that the incidence of abnormal cervical vertebral numbers in Late Pleisto

  10. THE TREATMENT AND EVOLUTION OF CERVICAL CANCER

    Directory of Open Access Journals (Sweden)

    Dragos Crauciuc

    2011-09-01

    Full Text Available The purpose of this study is to establish the evolution of cervical cancer after applying a conventional treatment. Materials and methods. The study was performed on a number of 1249 patients who were suspected of having cervical neoplasia, and who were monitored between 2006-2010 in „Elena-Doamna” Clinical Hospital of Obstetrics and Gynecology in Ia�i, the Military Hospital Gala�i, the County Hospital Gala�i and the Emergency Hospital Buzau. Results and discussions. The study proved the effectiveness of the conservative treatment for the patients who were diagnosed using cytology, colposcopy, biopsy and histopathology, with or without HPV viral infection. Conclusions. The patients with an early diagnose have a 15% higher surviving probability. The patients who responded to the conservative preoperative treatment well are more likely to survive than the patients who did not respond favourably to the conservative preoperative treatment.

  11. Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study

    Science.gov (United States)

    Bahena-Román, Margarita; Téllez-Sosa, Juan; Martínez-Barnetche, Jesús; Cortina-Ceballos, Bernardo; López-Estrada, Guillermina; Delgado-Romero, Karina; Burguete-García, Ana I.; Cantú, David; García-Carrancá, Alejandro; Madrid-Marina, Vicente

    2016-01-01

    Cervical cancer (CC) is caused by high-risk human papillomavirus persistence due to the immunosuppressive tumor microenvironment mediated by cytokines. Vaginal microbiota determines the presence of certain cytokines locally. We assessed the association between cervical microbiota diversity and the histopathological diagnosis of each stage of CC, and we evaluated mRNA cervical expression levels of IL-4, IL-6, IL-10, TGF-β1, TNF-α and IFN-γ across the histopathological diagnosis and specific bacterial clusters. We determined the cervical microbiota by high throughput sequencing of 16S rDNA amplicons and classified it in community state types (CST). Mean difference analyses between alpha-diversity and histopathological diagnosis were carried out, as well as a β-diversity analysis within the histological diagnosis. Cervical cytokine mRNA expression was analyzed across the CSTs and the histopathological diagnoses. We found a significant difference in microbiota's diversity in NCL-HPV negative women vs those with squamous intraepithelial lesions (SIL) and CC(p = 0.006, p = 0.036).When β-diversity was evaluated, the CC samples showed the highest variation within groups (p<0.0006) and the largest distance compared to NCL-HPV negative ones (p<0.00001). The predominant bacteria in women with normal cytology were L. crispatus and L. iners, whereas for SIL, it was Sneathia spp. and for CC, Fusobacterium spp. We found higher median cervical levels of IL-4 and TGF-β1 mRNA in the CST dominated by Fusobacterium spp. These results suggest that the cervical microbiota may be implicated in cervical cancer pathology. Further cohort studies are needed to validate these findings. PMID:27115350

  12. 2009~2013年鞍山地区农村妇女宫颈癌检查结果分析%The Analysis of Test Results of Cervical Cancer in Rural Areas of Anshan

    Institute of Scientific and Technical Information of China (English)

    乔玉娇

    2015-01-01

    Objective To understand the 35~64 years old rural women’s cervical cancer and precancerous disease situation, and the relations between them. Methods Using the traditional cervical smear TBS classiifcation report for screening cervical condition in 75 262 cases of rural women. Results 70 994 cases were negative (NSIL), while 4 268 cases suffered cytology abnormalities, among whom 2 507 cases were examined by colposcope and 86 cases had precancerous disease related to cervical cancer. Meanwhile, there were 13 cases of pathological diagnosis of cervical cancer, with a detection rate of 1.73/10 thousand. Conclusion Positive universality for the rural women cervical cancer screening could ifnd cervical cancer and its precursors diseases early, the traditional cervical smear TBS classiifcation check brought no pain, no trauma, and was simple to carry out, which could be used as a choice for mass cervical cancer census.%目的:了解鞍山市35~64岁农村妇女宫颈癌及癌前病变发病情况,及其相关性研究。方法采用传统的宫颈刮片TBS分级报告法对75262例农村妇女进行宫颈疾病筛查。结果其中,阴性(NSIL)70994例,细胞学异常4268例,其中进行阴道镜检查的2507例,宫颈癌前病变86例。病理诊断宫颈癌13例,检出率1.73/万。结论积极对农村妇女进行普遍性宫颈癌筛查能提早发现宫颈癌前病变、宫颈癌病患。采用传统宫颈刮片TBS分类法检查无痛苦、损伤小、简便易行,可作为群体性宫颈癌普查的首选。

  13. Primary HPV screening for cervical cancer prevention: results from European trials

    DEFF Research Database (Denmark)

    Lynge, Elsebeth; Rebolj, Matejka

    2009-01-01

    Six European, randomized, controlled trials that will compare human papillomavirus (HPV) testing with cytological testing for cervical screening are under way. We reviewed the results published so far to compare the benefits and costs for participating women. At baseline screening, use of HPV tes...

  14. EXPLOITING THE TOOTHPICK EFFECT OF THE CYTOBRUSH BY PLASTIC EMBEDDING OF CERVICAL SAMPLES

    NARCIS (Netherlands)

    BOON, ME; ZEPPA, P; OUWERKERKNOORDAM, E; KOK, LP

    1991-01-01

    The introduction of the combined spatula-Cytobrush sampling method has increased the diagnostic accuracy of cervical cytology. However, the smears from a small number of cases contained epithelial fragments dislodged by the "toothpick effect" of the Cytobrush. Due to their thickness, these epithelia

  15. Epidemiology and costs of cervical cancer screening and cervical dysplasia in Italy

    Directory of Open Access Journals (Sweden)

    Valle Sabrina

    2009-02-01

    Full Text Available Abstract Background We estimated the number of women undergoing cervical cancer screening annually in Italy, the rates of cervical abnormalities detected, and the costs of screening and management of abnormalities. Methods The annual number of screened women was estimated from National Health Interview data. Data from the Italian Group for Cervical Cancer Screening were used to estimate the number of positive, negative and unsatisfactory Pap smears. The incidence of CIN (cervical intra-epithelial neoplasia was estimated from the Emilia Romagna Cancer Registry. Patterns of follow-up and treatment costs were estimated using a typical disease management approach based on national guidelines and data from the Italian Group for Cervical Cancer Screening. Treatment unit costs were obtained from Italian National Health Service and Hospital Information System of the Lazio Region. Results An estimated 6.4 million women aged 25–69 years undergo screening annually in Italy (1.2 million and 5.2 million through organized and opportunistic screening programs, respectively. Approximately 2.4% of tests have positive findings. There are approximately 21,000 cases of CIN1 and 7,000–17,000 cases of CIN2/3. Estimated costs to the healthcare service amount to €158.5 million for screening and €22.9 million for the management of cervical abnormalities. Conclusion Although some cervical abnormalities might have been underestimated, the total annual cost of cervical cancer prevention in Italy is approximately €181.5 million, of which 87% is attributable to screening.

  16. Cervical dystonia: a disorder of the midbrain network for covert attentional orienting.

    OpenAIRE

    MichaelHutchinson; TadashiIsa; FionaMolloy; HelenaMoore; DanielGHealy; TimLynch; CathalWalsh; JohnButler; RichardWalsh

    2014-01-01

    While the pathogenesis of cervical dystonia remains unknown, recent animal and clinical experimental studies have indicated its probable mechanisms. Abnormal temporal discrimination is a mediational endophenotype of cervical dystonia and informs new concepts of disease pathogenesis. Our hypothesis is that both abnormal temporal discrimination and cervical dystonia are due to a disorder of the midbrain network for covert attentional orienting caused by reduced gamma-aminobutyric acid inhibitio...

  17. Cervical Dystonia: A Disorder of the Midbrain Network for Covert Attentional Orienting

    OpenAIRE

    Hutchinson, Michael; Isa, Tadashi; Molloy, Anna; Kimmich, Okka; Williams, Laura; Molloy, Fiona; Moore, Helena; Healy, Daniel G; Lynch, Tim; Walsh, Cathal; Butler, John; Richard B. Reilly; Walsh, Richard; O’Riordan, Sean

    2014-01-01

    While the pathogenesis of cervical dystonia remains unknown, recent animal and clinical experimental studies have indicated its probable mechanisms. Abnormal temporal discrimination is a mediational endophenotype of cervical dystonia and informs new concepts of disease pathogenesis. Our hypothesis is that both abnormal temporal discrimination and cervical dystonia are due to a disorder of the midbrain network for covert attentional orienting caused by reduced gamma-aminobutyric acid (GABA) in...

  18. Laminoplasty for Cervical Myelopathy

    OpenAIRE

    Ito, Manabu; Nagahama, Ken

    2012-01-01

    This article reviews cervical laminoplasty. The origin of cervical laminoplasty dates back to cervical laminectomy performed in Japan ~50 years ago. To overcome poor surgical outcomes of cervical laminectomy, many Japanese orthopedic spine surgeons devoted their lives to developing better posterior decompression procedures for the cervical spine. Thanks to the development of a high-speed surgical burr, posterior decompression procedures for the cervical spine showed vast improvement from the ...

  19. Cytology of 2n Pollen Formation in Nonastringent Persimmon

    Institute of Scientific and Technical Information of China (English)

    TANG Xian-ying; LUO Zheng-rong

    2002-01-01

    Cytological mechanisms of 2n pollen formation in ‘Zenjimaru' nonastringent persimmon (Diospyros kaki L.f. ) were studied. The following abnormalities in meiosis were found to be responsible for the production of 2n pollens: (1) disoriented spindles, including parallel, fused and tripolar spindles, were formed at metaphase Ⅱ and anaphase Ⅱ; (2) the nuclei at telophase Ⅱ were arranged to two poles, each of which contained two nuclei, or to three poles, one of which contained two nuclei, the other two contained one nucleus respectively; (3) dyads and triads were produced at the tetrad stage. The dyad would develop into two 2n pollens, and the triad would develop into one 2n and two n pollens. The 2n pollens produced by this mechanism were genetically equivalent to FDR (first division restitution) gametes, thus providing a potential value for sexual polyploidization.

  20. Risk-factors and strategies for control of carcinoma cervix in India: Hospital based cytological screening experience of 35 years

    Directory of Open Access Journals (Sweden)

    J S Misra

    2009-01-01

    Full Text Available Purpose: Role of risk factors in cervical carcinogenesis and strategies for control of the disease have been assessed from the accumulated cytological data, derived from 35 years of hospital-based screening in Lucknow, North India. Materials and Methods: A total of 36,484 women have been cytologically screened during a span of 35 years (April 1971 - June 2005 in the Gynaecology out patient department (OPD of Queen Mary′s Hospital. Results: The frequency of Squamous Intraepithelial Lesion (SIL and carcinoma was found to be 7.2% and 0.6%, respectively, in the present study. The study revealed high age and parity as a predominant factor in cervical carcinogenesis, while viral sexually transmitted disease (STDs -human papilloma virus (HPV and Herpes simplex virus (HSV were also largely associated with SIL cases. The study emphasized great value of clinically downstaging the cervical cancer by detecting cervical cancer in the early stage. The study also revealed a significant difference in the frequency of SIL in symptomatic and asymptomatic women. Conclusion: Based on the analyzed data, it was felt that single lifetime screening, which appears to be the most feasible and affordable mode for control of carcinoma cervix in developing countries like India, should be carried out in all women of high parity irrespective of age (with three or more children and in older women above the age of 40 years irrespective of parity.

  1. An special epithelial staining agents: folic acid receptor-mediated diagnosis (FRD) effectively and conveniently screen patients with cervical cancer.

    Science.gov (United States)

    Lu, Meng-Han; Hu, Ling-Yun; Du, Xin-Xin; Yang, Min; Zhang, Wei-Yi; Huang, Ke; Li, Li-An; Jiang, Shu-Fang; Li, Ya-Li

    2015-01-01

    High-quality screening with cytology has markedly reduced mortality from cervical cancer. However, it needs experienced pathologists to review and make the final decisions. We have developed folic acid receptor-mediated diagnosis (FRD) kits to effectively and conveniently screen patients with cervical cancer. We conduct present study aim to assess clinical significances of FRD in screening cervical cancer. A total of 169 patients were enrolled at Chinese People's liberation Army (PLA) general hospital. We compared diagnostic significances of FRD with thinprep cytology test (TCT). Meanwhile, colposcopy was also performed to confirm any lesion suspicious for cervical cancer. The sensitivity and specificity of FRD were 71.93% and 66.07% in diagnosis cervical cancer, respectively. Meanwhile, the positive predictive values (PPV), negative predictive values (NPV), Youden index were 51.90%, 82.22%, 0.38, respectively. On the other hand, the sensitivity and specificity of TCT in diagnosis cervical cancer were 73.68% and 61.61% respectively. PPV, NPV and Youden index for TCT were 49.41%, 82.14% and 0.35 respectively. Overall, FRD have high values of sensitivity, specificity and Youden index. However, this difference failed to statistical significance. FRD have comparable diagnostic significance with TCT. Therefore, FRD might serve as one effective method to screen cervical cancer. Especially for those patients living in remote regions of China, where cytology was unavailable.

  2. [Cervical radiculopathy].

    Science.gov (United States)

    Kuijper, B

    2014-10-01

    Cervical radiculopathy is a common cause of pain in the arm. It is caused by nerve root compression in the neck, as a consequence of a herniated disc, or spondyliotic foraminal stenosis. It causes severe pain, especially during the first few weeks, and paraesthesias in the forearm and hand. Patients also suffer from neck pain and loss of strength in the relevant arm. The arm pain can be exacerbated by certain movements of the head; these should be avoided as much as possible. Diagnosis can be made on the basis of history and physical examination. The pain generally disappears without active patient treatment. A semi-rigid cervical collar is recommended to accelerate pain relief. In cases of persistent pain, surgery will be considered. In such cases an MRI should be performed to show the cause and level of nerve root compression. PMID:26185991

  3. MICROARRAY ANALYSIS OF DIFFERENT GENE EXPRESSION OF HUMAN CERVICAL CANCER SUBCLONE CELL LINES

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Cervical cancer is the main cause of death inwomen.The influence of HPV plays an i mportantrole incervial cancer.It has been provedthat humanpapillomavirus(HPV)infectionis ani mportant fac-tor in cervical carcinogenesis.Multiple HPVinfec-tion was associated less frequently with cervical car-cinoma and with precancerous lesions compared withnor mal cytology[1].The activation of oncogene,in-activition of tumor suppressor gene and instabilityof genome are also majority reason.We establisheda cell line of human...

  4. Cervical Dysplasia and High-Risk Human Papillomavirus Infections among HIV-Infected and HIV-Uninfected Adolescent Females in South Africa

    Directory of Open Access Journals (Sweden)

    David H. Adler

    2014-01-01

    In this cross-sectional study, we compared the HPV DNA and Pap smear results between 35 HIV-infected and 50 HIV-uninfected adolescents in order to determine the prevalence of HR-HPV genotypes and cervical cytological abnormalities. Comparisons were made using Pearson χ2 and independent-samples t-tests analyses, and associations between demographic and behavioral characteristics and HPV infections were examined. Results. HIV-infected participants were more likely to be infected with any HPV (88.6% versus 48.0%; P<0.001 and with at least one HR-HPV (60.0% versus 24.0%; P=0.001, and to have multiple concurrent HPV infections (68.6% versus 22.0%; P<0.001. HPV 16 and 18 were relatively underrepresented among HR-HPV infections. Abnormal Pap test results were more common among HIV-infected participants (28.8% versus 12.0%; P=0.054. A history of smoking was associated with HR-HPV infection. Conclusions. HIV-infected adolescents have an increased risk of infection with HR-HPV and of Pap test abnormalities. The majority of HR-HPV infections among our participants would not be prevented by the currently available vaccinations against HPV.

  5. Assessment of effectiveness and cost-effectiveness of HPV testing in primary screening for cervical

    Directory of Open Access Journals (Sweden)

    Willich, Stefan N.

    2007-09-01

    Full Text Available Introduction: The introduction of a screening programme for cervical carcinoma in Germany has led to a significant reduction in incidence of the disease. To date, however, diagnosis in Germany has been based solely on cervical cytology, which has been criticised because of a low sensitivity and consequently high rate of false negative results. Because an infection with the human papillomavirus (HPV previously was found to be a necessary aetiological factor in the development of cervical cancer, there has been some discussion that HPV testing should be included in cervical cancer screening. Objectives: How do HPV tests compare to cytological tests in terms of sensitivity and specificity, and what are the effects of screening for cervical carcinoma in Germany? Is there health economic evidence that may foster an inclusion of HPV testing into national screening programms? Methods: A systematic literature review was performed, including studies that compared the HPV test to cervical cytology in terms of sensitivity and specificity in the diagnosis of CIN 2+ (CIN=Cervical Intraepithelial Neoplasia. In addition, a systematic review of the relevant health economic literature was performed to analyze cost-effectiveness in the German setting. Results: A total of 24 studies fulfilled the inclusion criteria. One study consisted of three substudies. Hence, results of 26 comparisons of HPV and cytology are reported. In 25 of these, the HPV test was more sensitive than cytology, whereas cytology had better specificity in 21 studies. The combination of HPV test and cytology increased sensitivity. Variability in results was considerably larger for cytology than for HPV testing. Results of the economic meta-analysis suggest that in health care settings with already established PAP screening programms, cost-effectiveness strongly depends on screening intervals. In analyses comparing HPV screening to conventional PAP screening with two-yearly intervals, only 25

  6. Cytology result entry without using the keyboard.

    Science.gov (United States)

    Aller, R D; DeWitt, C

    1994-01-01

    Many keystrokes are needed to enter routine gynecologic cytology results into many computer systems. Not only is this quite time consuming, but typing errors cannot be avoided and are not always detected before the report is sent to the clinician. Because of continuing shortages of staff time and the desire to avoid transcription errors, we began using $400 bar code wands to enter cytology results and billing information. No changes in our laboratory information system software or tables were necessary. Accession number labels are preprinted on a stand-alone printer. Bar code sheets, containing common keystroke sequences, are printed using a standard Macintosh computer and Laserwriter printer. Scanning the bar codes exactly emulates those keystrokes typed on the keyboard. We have entered the results of > 99% of our gynecologic cytology cases in the past two years without touching the keyboard. PMID:8091908

  7. Fine-needle aspiration cytology in fibromatoses.

    Science.gov (United States)

    Zaharopoulos, P; Wong, J Y

    1992-01-01

    Fine-needle aspiration (FNA) cytology was performed in seven cases of fibromatosis of variable types with tumorous clinical presentation. These included: four cases of musculoaponeurotic fibromatosis, two in posterior neck muscles, one in anterior neck muscles and one in intercostal muscles; one case of fibromatosis of the breast; and two cases of fibromatosis colli in neonates. In all cases the specimens contained connective tissue with many fibroblast-like cells, lacking features which could indicate a malignant lesion. The findings in these cases indicate that, although by FNA cytology in fibromatoses a specific diagnosis for each pathologic entity may not be easily reached, in the proper clinical setting the cytologic findings can be of sufficient relevance to offset the need for an open tissue biopsy, where there are valid reasons against a surgical intervention.

  8. Diagnostic accuracy and