Sample records for abies guatemalensis rehder


    José Vicente Martínez-Arévalo


    Full Text Available The Chemical composition ( pH , elements major and minor , % S.B., CIC , % M.O. and N.T on soils in open areas and forest fir (Abies guatemalensis Rehder in a successional gradient was studied. These results were related to vegetation through correlation analysis and Canonical Correspondence analysis. The aim was to show the relationships present between vegetation and soil chemistry through ecological succession in areas of cold climate. The results indicate that the vegetation is composed of 82 species of which 20 are the lower herbaceous layer, 34 the herbaceous layer of the upper, 21 shrubs and seven trees. The chemical characteristics of the soil through five successional stages have only few significant differences and not show a clear tendency to increase according to the degree of ecosystem development. A low percentage of correlations between vegetation and soil chemical characteristics are presented. The correspondence canonical analysis shows that the features that correlate with sites through vegetation are the K, and % S.B. and CIC. One can conclude that the approach of the study of the chemical composition of soils in areas of ecological sequence is complex, especially in high regions where, besides other factors, the temperature and humidity play an important role on the soil-vegetation dynamic.

  2. Los bosques de Abies guatemalensis Rehder de San Marcos, Guatemala: una oportunidad para su restauración ecológica

    José Vicente Martínez Arévalo


    Full Text Available El pinabete, Abies guatemalensis Rehder, es una especie distribuida en las montañas altas de Guatemala. Según el Consejo Nacional de Áreas Protegidas (Conap, sus poblaciones se encuentran severamente fragmentadas. Por su rareza y fragmentación, esta especie se encuentra protegida por el Convenio sobre el Comercio Internacional de Especies Amenazadas de Fauna y Flora Silvestre (Cites, lo que pone en relieve la importancia de la recuperación de sus bosques para su debida conservación. El éxito de su establecimiento puede mejorase al sembrarla junto a plantas nodriza, que le ayudarían a sobrevivir a las condiciones secas del año y las heladas que se presentan en las zonas de mayor altitud del país durante los meses de noviembre a febrero. Este trabajo muestra las características físicas, químicas y de vegetación del área de tres bosques de pinabete (A. guatemalensis de la parte alta de San Marcos. Además, presenta la propuesta de restauración ecológica estimulada simulando la sucesión vegetal del área. Se elaboraron mapas, y describieron tres bosques de pinabete y sus alrededores; se presenta el estudio de la composición química del suelo y la vegetación y su representación en diferentes etapas de sucesión vegetal. Además, se propone la recuperación de áreas alrededor de parches de bosque, consistente en establecer plantas arbustivas en lugares abiertos donde aún no hay; por su parte en aquellas áreas donde ya se encuentren arbustos, se propone utilizarlos como plantas nodriza para establecer árboles de varias especies buscando mantener biodiversidad de especies arbóreas.

  3. Leaf and fruit essential oil compositions of Pimenta guatemalensis (Myrtaceae) from Costa Rica.

    Chaverri, Carlos; Cicció, José F


    Pitnenta is a genus of flowering plants in the Myrtaceae family, which has about 15 species, mostly found in the Caribbean region of the Americas. Commonly used for culinary and medicinal purposes, the best known commercial species are allspice, P. dioica (P. officinalis) and bay rum, P. racemosa, but there is little information concerning P. guatemalensis. The aim of the present study was to identify the chemical composition of the leaf and fruit essential oils ofP. guatemalensis. The extraction of essential oils of P. guatemalensis growing wild in Costa Rica was carried out by the hydrodistillation method at atmospheric pressure, using a modified Clevenger type apparatus. The chemical composition of the oils was analyzed by capillary gas chromatographyflame ionization detector (GC/FID) and gas chromatography-mass spectrometry (GC/MS) using the retention indices on DB-5 type capillary column. A total of 103 and 63 compounds were identified in the leaf and fruit oils, respectively, corresponding to 96.8% and 86.1% of the total amount of the oils. The leaf oil consisted mainly of eugenol (72.8%), and mono- and sesquiterpene hydrocarbons (18.2%). Among terpenes the major components were beta-caryophyllene (8.2%) and terpinolene (3.0%). The fruit oil also consisted mainly of eugenol (74.7%) and minor amounts of oxygenated mono- and sesquiterpenes (7.3%), mainly caryophyllene oxide (3.3%). This is the first report of the chemical composition of the essential oils obtained from this plant species.

  4. Antinociceptive effect of aqueous extracts from the bark of Croton guatemalensis Lotsy in mice

    Rejón-Orantes José del Carmen


    Full Text Available Croton guatemalensis Lotsy (CGL, known as "copalchi" in Chiapas, Mexico, is used for the treatment of fever, abdominal pain and malaria and also as a remedy for chills and for treating rheumatism. The aim of this study was to evaluate whether aqueous extracts from the bark of this plant possesses indeed antinociceptive properties by using two different animal models of nociception, the acetic acid-induced writhing test and the hot plate model. The results showed that i.p. administration of this extract (0, 100, 200 and 400 mg/kg 30 min prior testing had significant dose-dependent antinociceptive effects in the acetic acid-induced writhing test and that the reduction of writhings (85.5 % as compared to the control at the highest dose tested is similar to that exhibited by dipyrone (250 mg/kg. This effect was not reversed by naloxone, a non-selective opioid receptor antagonist, suggesting that the endogenous opioid system does not underlie the antinociceptive effects of CGL in the acetic acid-induced writhing test. No effects were however observed in the hot-plate model. Our results indicate that aqueous extracts from Croton guatemalensis bark contain pharmacologically active constituents endowed with antinociceptive activity. It is suggested that cyclooxygenase inhibition might be at least partially involved in the antinociceptive effects of this extract.

  5. Antinociceptive effect of aqueous extracts from the bark of Croton guatemalensis Lotsy in mice.

    Del Carmen, Rejón-Orantes José; Willam, Hernández Macías John; Del Carmen, Grajales Morales Azucena; Nataly, Jiménez-García; Stefany, Coutiño Ochoa Samantha; Anahi, Cañas Avalos; Domingo, Parcero Torres Jorge; Leonardo, Gordillo Páez; Miguel, Pérez de la Mora


    Croton guatemalensis Lotsy (CGL), known as "copalchi" in Chiapas, Mexico, is used for the treatment of fever, abdominal pain and malaria and also as a remedy for chills and for treating rheumatism. The aim of this study was to evaluate whether aqueous extracts from the bark of this plant possesses indeed antinociceptive properties by using two different animal models of nociception, the acetic acid-induced writhing test and the hot plate model. The results showed that i.p. administration of this extract (0, 100, 200 and 400 mg/kg) 30 min prior testing had significant dose-dependent antinociceptive effects in the acetic acid-induced writhing test and that the reduction of writhings (85.5 % as compared to the control) at the highest dose tested is similar to that exhibited by dipyrone (250 mg/kg). This effect was not reversed by naloxone, a non-selective opioid receptor antagonist, suggesting that the endogenous opioid system does not underlie the antinociceptive effects of CGL in the acetic acid-induced writhing test. No effects were however observed in the hot-plate model. Our results indicate that aqueous extracts from Croton guatemalensis bark contain pharmacologically active constituents endowed with antinociceptive activity. It is suggested that cyclooxygenase inhibition might be at least partially involved in the antinociceptive effects of this extract.

  6. Compositional Variation and Bioactivity of the Leaf Essential Oil of Montanoa guatemalensis from Monteverde, Costa Rica: A Preliminary Investigation

    Victoria D. Flatt


    Full Text Available Background: Montanoa guatemalensis is a small to medium-sized tree in the Asteraceae that grows in Central America from Mexico south through Costa Rica. There have been no previous investigations on the essential oil of this tree. Methods: The leaf essential oils of M. guatemalensis were obtained from different individual trees growing in Monteverde, Costa Rica, in two different years, and were analyzed by gas chromatography—mass spectrometry. Results: The leaf oils from 2008 were rich in sesquiterpenoids, dominated by α-selinene, β-selinene, and cyclocolorenone, with lesser amounts of the monoterpenes α-pinene and limonene. In contrast, the samples from 2009 showed no α- or β-selinene, but large concentrations of trans-muurola-4(14,5-diene, β-cadinene, and cyclocolorenone, along with greater concentrations of α-pinene and limonene. The leaf oils were screened for cytotoxic and antimicrobial activities and did show selective cytotoxic activity on MDA-MB-231 breast tumor cells. Conclusion: M. guatemalensis leaf oil, rich in cyclocolorenone, α-selinene, and β-selinene, showed selective in vitro cytotoxic activity to MDA-MB-231 cells. The plant may be a good source of cyclocolorenone.

  7. Illustrations and studies in Neotropical Orchidaceae. 6. The Lepanthes guatemalensis group (Pleurothallidinae in Costa Rica

    Pupulin, Franco


    Full Text Available We revise and characterize the group of species close to Lepanthes guatemalensis. The group comprises eight species in Costa Rica. Three new species are described and illustrated. Lepanthes durikäensis is distinguished by the non resupinate flowers, the lateral sepals ovate with the apical tails diverging, the lower lobe of petals lanceolate-subfalcate, up-curved and the pinkish column. Lepanthes leporina is recognized by the resupinate flowers with yellow sepals, faintly suffused with red, the petals violet, the lip orange, the bifid synsepal with the apices of the lateral sepals not forming tails, the lateral sepals subequal to the dorsal sepal, the petals markedly convex with the upper lobe elliptic-lanceolate and the lobes of the lip connivent at apex. Lepanthes sanctiorum is characterized by the small plants less than 1.1 cm tall, the short peduncle less than 10 mm long, the petals as wide as the lip length and the blades of the lip distinctly diverging at apex. All the species are described and illustrated on the basis of Costa Rican material. A key to the species of the group is provided.Revisamos y caracterizamos el grupo de especies cercanas a Lepanthes guatemalensis. El grupo está compuesto por ocho especies en Costa Rica. Se describen e ilustran tres especies nuevas. Lepanthes durikäensis se caracteriza por las flores no resupinadas, los sépalos laterales ovados, con las colas apicales divergentes, el lóbulo inferior de los pétalos lanceolado-subfalcado, con curvatura hacia arriba y la columna rosada. Lepanthes leporina es reconocida por las flores resupinadas, los sépalos amarillos y ligeramente teñidos de rojo, los pétalos violeta, el labelo naranja, el sinsépalo bífido con los ápices de los sépalos laterales que no forman colas, los sépalos laterales subiguales al sépalo dorsal, los pétalos marcadamente convexo con el lóbulo superior elíptico-lanceolado y los lóbulos del labelo conniventes en el ápice. Lepanthes

  8. Allelopathy on bark of downed logs of Chamaecyparis Obtusa sieb. and Zucc. var. formosana (Hayata) Rehder.

    Tseng, Mei-Hwei; Lai, Wen-Rong; Hsieh, Chin-Lin; Kuo, Yueh-Hsiung


    Chamaecyparis obtusa Sieb. and Zucc. var. formosana (Hayata) Rehder is the dominant species in the temperate forest of Yuanyang Lake Nature Reserve (YYL), Taiwan. Although downed logs of C. obstusa var. formosana occupy only a small percentage of the forest floor area in YYL, they are important regeneration substrates. Seedlings of this species often grow without competition on the new downed logs, and a few broadleaf trees grow with them. We hypothesized that the bark of the newly fallen logs possesses allelopathic potential that provides a habitat especially suitable for seedling establishment. Eight different seeds including those from Lactuca sativa L. (lettuce), Bidens pilosa (an invasive weed), and six species in YYL were planted on the bark of the downed logs in an incubator for germination tests. Two dominant species in the forest of YYL, C. obtusa var. formosana and Rhododendron formosanum, were able to grow normally, but the others, Pieris taiwanensis, Barthea formosana, Chamaecyparis formosensis, Miscanthus transmorrisonensis, lettuce, and B. pilosa were growth inhibited. A bioactivity-guided isolation was designed to isolate allelochemicals from the bark. Salicylic acid, one of the inhibiting substances, was isolated and identified by gas chromatography/mass spectroscopy (GC/MS), proton nuclear magnetic resonance ((1)H NMR), and infrared (IR). Bioassay of salicylic acid confirmed a phytotoxic effect. The results suggest that the dominance of C. obtusa var. formosana seedlings on bark could be partly due to allelopathy.

  9. Análisis genotípico en Vochysia guatemalensis Donn Smith (Vochysiaceae) mediante microsatélites

    Rojas, Fabiana; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica; Murillo-Gamboa, Olman; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica; Aguilar, Gabriel; Universidad de Costa Rica; Rocha, Oscar; Kent State University, Ohio, USA; Araya-Valverde, Emanuel; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica


    En el presente estudio se muestran los resultados del análisis de una colección de 62 clones de Vochysia guatemalensis ubicados en la zona norte y caribe de Costa Rica mediante el uso microsatélites (SSR), adaptados y validados de un grupo de marcadores existentes para Vochysia ferruginea. El material fueseleccionado de árboles plus procedente de varias poblaciones naturales y de pequeñas plantaciones. Se trabajó en el establecimiento de la huella genética de cada clon y en el agrupamiento de...

  10. Cytotoxic terpenes from Abies sibirica

    Gui Rong Wang; Yong Li Li; Wei Dong Zhang; Xian Wen Yang; Wen Cong Liu; Ji Ye; Zhi Jun Zhu; Hong Chen


    One new and 16 known compounds were isolated from Abies sibirica.Their structures were assigned mainly on the basis ofNMR and MS spectroscopic data.In bioassay for anti-proliferative activity against four human tumor cells,compound 7 exhibitedselective anti-proliferative activity against COLO-205 rumor cell with an IC50 value of 0.9 μg/mL.

  11. A New Farnesyl Diphosphate Synthase Gene from Taxus media Rehder: Cloning, Characterization and Functional Complementation

    Zhi-Hua Liao; Min Chen; Yi-Fu Gong; Zhu-Gang Li; Kai-Jing Zuo; Peng Wang; Feng Tan; Xiao-Fen Sun; Ke-Xuan Tang


    Farnesyl diphosphate synthase (FPS; EC catalyzes the production of 15-carbon farnesyl diphosphate which is a branch-point intermediate for many terpenoids. This reaction is considered to be a ratelimiting step in terpenoid biosynthesis. Here we report for the first time the cloning of a new full-length cDNA encoding farnesyl diphosphate synthase from a gymnosperm plant species, Taxus media Rehder,designated as TmFPS1. The full-length cDNA of TmFPS1 (GenBank accession number: AY461811) was 1 464bp with a 1 056-bp open reading frame encoding a 351-amino acid polypeptide with a calculated molecular weight of 40.3 kDa and a theoretical pl of 5.07. Bioinformatic analysis revealed that TmFPS1 contained all five conserved domains of prenyltransferases, and showed homology to other FPSs of plant origin. Phylogenetic analysis showed that farnesyl diphosphate synthases can be divided into two groups: one of prokaryotic origin and the other of eukaryotic origin. TmFPS1 was grouped with FPSs of plant origin. Homologybased structural modeling showed that TmFPS1 had the typical spatial structure of FPS, whose most prominent structural feature is the arrangement of 13 core helices around a large central cavity in which the catalytic reaction takes place. Our bioinformatic analysis strongly suggests that TmFPS1 is a functional gene. Southern blot analysis revealed that TmFPS1 belongs to a small FPS gene family in T. media. Northern blot analysis indicated that TmFPS1 is expressed in all tested tissues, including the needles, stems and roots of T. media. Subsequently, functional complementation with TmFPS1 in a FPS-deficient mutant yeast demonstrated that TmFPS1 did encode farnesyl diphosphate synthase, which rescued the yeast mutant.This study will be helpful in future investigations aiming at understanding the detailed role of FPS in terpenoid biosynthesis flux control at the molecular genetic level.

  12. Autecology and conservation status of Magnolia sargentiana Rehder & Wilson (Magnoliaceae) in the Dafengding region, southern Sichuan Province, China

    Jing WANG; Ya TANG; Zheng-Hua XIE; Mian-Yue ZHANG


    This paper reports the first population ecology study of the endangered Magnolia sargentiana Rehder & Wilson (Magnoliaceae). Magnolia sargentiana is a protected species in China, but little is known about its present status in the field. In 2007 and 2008, we surveyed the population and conservation status of M. sargentiana in the Provincial Mamize Nature Reserve and the National Meigu Dafengding Nature Reserve, Sichuan Province, southwestern China. Natural regeneration is poor because of unfavorable environmental conditions and anthropogenic disturbances. Flower buds and bark of M. sargentiana are used in traditional Chinese medicine and their collection by local people over the period 1983-1994 has led to marked population declines. The collection of flower buds and bark is now banned, but hewing branches for firewood and grazing continues to have a negative impact on the recovery of M. sargentiana populations. To protect the species, we require a ban on hewing branches, closure of primary forests to reduce the impact of humans and ungulates, better education of local people, and increased awareness of wildlife conservation.

  13. Potential of AbiS as defence mechanism determined by conductivity measurement

    Holubová, Jitka; Josephsen, Jytte


    Aim: To compare pH and conductivity used in the determination of growth in reconstituted skim milk (RSM), to determine whether the presence of one or two plasmids in Lactococcus lactis had any influence on growth, and whether AbiS improved bacteriophages resistance of L. lactis. Methods and Results...

  14. 19 CFR 143.7 - Revocation of ABI participation.


    ... THE TREASURY (CONTINUED) SPECIAL ENTRY PROCEDURES Automated Broker Interface § 143.7 Revocation of ABI..., Trade Policy and Programs, Office of International Trade, will immediately revoke ABI participation....

  15. The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in Arabidopsis.

    Sugliani, M.; Brambilla, V.; Clerkx, E.J.M.; Koornneef, M.; Soppe, W.J.J.


    ABSCISIC ACID INSENSITIVE3 (ABI3) is a major regulator of seed maturation in Arabidopsis thaliana. We detected two ABI3 transcripts, ABI3- and ABI3-ß, which encode full-length and truncated proteins, respectively. Alternative splicing of ABI3 is developmentally regulated, and the ABI3-ß transcript a

  16. Factores edáficos que influyen en el crecimiento de Vochysia guatemalensis en la región Huetar Norte de Costa Rica

    Mora, Fernando; Muñoz, Roger; Meza, Víctor; Fonseca, William


    Se evalúa el crecimiento de Vochysia guatemalensis en Ultisoles e Inceptisoles de la Región Huetar Norte de Costa Rica. Este documento presenta las condiciones de los sitios donde la especie está creciendo en la región bajo estudio. Por medio de variables físicas y químicas de los suelos, se explicó el comportamiento del índice de sitio (IS) y su relación con aquellas variables edáficas que muestran algún tipo de relación con este indicador de la calidad de los terrenos. Además, se determinar...

  17. Proteocephalus brooksi n. sp. (Cestoda:Proteocephalidae) in the neotropical freshwater fish Rhamdia guatemalensis (Siluriformes: Pimelodidae) from Lake Catemaco, Veracruz, Mexico.

    García-Prieto, L; Rodríguez, L M; Pérez-Ponce de León, G


    Proteocephalus brooksi n. sp. is described from the neotropical pimelodid fish Rhamdia guatemalensis from Lake Catemaco, Veracruz, México. The new species is characterized by the "paramuscular" position of vitellaria, the cortical origin of uterine stem with development of medullar lateral branches, the alternated position of vagina (anteriorly and posteriorly to cirrus sac), and the absence of apical organ and vaginal sphincter. Proteocephalus brooksi most closely resembles Nomimoscolex matogrossensis from which it differs in a series of characters. The probable paraphyletic nature of both Proteocephalus and Nomimoscolex and the convergent evolution of the "paramuscular" location of vitellaria among proteocephalideans are suggested, and the need for a phylogenetic analysis of the group is emphasized. The new species is assigned to Proteocephalus pending such an analysis.

  18. Detector level ABI spectral response function: FM4 analysis and comparison for different ABI modules

    Efremova, Boryana; Pearlman, Aaron J.; Padula, Frank; Wu, Xiangqian


    A new generation of imaging instruments Advanced Baseline Imager (ABI) is to be launched aboard the Geostationary Operational Environmental Satellites - R Series (GOES-R). Four ABI flight modules (FM) are planned to be launched on GOES-R,S,T,U, the first one in the fall of 2016. Pre-launch testing is on-going for FM3 and FM4. ABI has 16 spectral channels, six in the visible/near infrared (VNIR 0.47 - 2.25 μm), and ten in the thermal infrared (TIR 3.9 - 13.3 μm) spectral regions, to be calibrated on-orbit by observing respectively a solar diffuser and a blackbody. Each channel has hundreds of detectors arranged in columns. Operationally one Analytic Generation of Spectral Response (ANGEN) function will be used to represent the spectral response function (SRF) of all detectors in a band. The Vendor conducted prelaunch end-to-end SRF testing to compare to ANGEN; detector specific SRF data was taken for: i) best detector selected (BDS) mode - for FM 2,3, and 4; and ii) all detectors (column mode) - for four spectral bands in FM3 and FM4. The GOES-R calibration working group (CWG) has independently used the SRF test data for FM2 and FM3 to study the potential impact of detector-to-detector SRF differences on the ABI detected Earth view radiances. In this paper we expand the CWG analysis to include the FM4 SRF test data - the results are in agreement with the Vendor analysis, and show excellent instrument performance and compare the detector-to-detector SRF differences and their potential impact on the detected Earth view radiances for all of the tested ABI modules.


    Ottaviano Allegretti,


    Full Text Available The study presents results of the characterization of Norway spruce (Picea abies Karst. and fir (Abies alba Mill. wood thermally modified by TERMOVUOTO® technology at temperatures in the range of 160 to 220°C in vacuum conditions. Sixteen thermo-vacuum treatment tests were carried out using a pilot laboratory unit on 30-mm-thick spruce and fir boards in various combinations of the process parameters, i.e. temperature (T, duration (t, and pressure (p. The treated material was characterized to reveal the changes of the physical-mechanical properties including color and durability. The treated wood showed an improved performance with relation to the dimensional stability and durability. The measured mechanical properties did not show any significant decrease. Analytical models, based on the existing correlations between wood properties and process parameters, were assessed, thus allowing the control of the process.

  20. Separation of polyphenols from leaves of Malus hupehensis (Pamp.) Rehder by off-line two-dimensional High Speed Counter-Current Chromatography combined with recycling elution mode.

    Liu, Qi; Zeng, Hualiang; Jiang, Shujing; Zhang, Li; Yang, Fuzhu; Chen, Xiaoqing; Yang, Hua


    In this study, off-line two-dimensional High Speed Counter-Current Chromatography (2D HSCCC) strategy combined with recycling elution mode was developed to isolate compounds from the ethyl acetate extract of a common green tea--leaves of Malus hupehensis (Pamp.) Rehder. In the orthogonal separation system, a conventional HSCCC was employed for the first dimension and two recycling HSCCCs were used for the second in parallel. Using a solvent system consisting of n-hexane-ethyl acetate-methanol-water (1:4:0.6:4.4, v/v) in the first and second dimension, four compounds including 3-hydroxy-phlorizin (1), phloretin (2), avicularin (3) and kaempferol 3-O-β-D-glucoside (4) were obtained. The purities of these four compounds were all over 95.0% as determined by HPLC. And their structures were all identified through UV, MS and (1)H NMR. It has been demonstrated that the combination of off-line 2D HSCCC with recycling elution mode is an efficient technique to isolate compounds with similar polarities in natural products.

  1. A social identity approach to acquired brain injury (ABI)

    Walsh, Stephen R.


    peer-reviewed The central argument put forward in this thesis is that, in the context of acquired brain injury (ABI) social identity matters. The first article is a theoretical paper which reviews an emerging literature that is trying to draw together social psychology and neuropsychology in the study of ABI. This article argues that the social identity approach is an appropriate vehicle for such integration and introduces the concept of identity sub-types based on belonging and based on p...

  2. ABI3 ectopic expression reduces in vitro and in vivo cell growth properties while inducing senescence

    Riggins Gregory J


    Full Text Available Abstract Background Mounting evidence has indicated that ABI3 (ABI family member 3 function as a tumor suppressor gene, although the molecular mechanism by which ABI3 acts remains largely unknown. Methods The present study investigated ABI3 expression in a large panel of benign and malignant thyroid tumors and explored a correlation between the expression of ABI3 and its potential partner ABI3-binding protein (ABI3BP. We next explored the biological effects of ABI3 ectopic expression in thyroid and colon carcinoma cell lines, in which its expression was reduced or absent. Results We not only observed that ABI3 expression is reduced or lost in most carcinomas but also that there is a positive correlation between ABI3 and ABI3BP expression. Ectopic expression of ABI3 was sufficient to lead to a lower transforming activity, reduced tumor in vitro growth properties, suppressed in vitro anchorage-independent growth and in vivo tumor formation while, cellular senescence increased. These responses were accompanied by the up-regulation of the cell cycle inhibitor p21 WAF1 and reduced ERK phosphorylation and E2F1 expression. Conclusions Our result links ABI3 to the pathogenesis and progression of some cancers and suggests that ABI3 or its pathway might have interest as therapeutic target. These results also suggest that the pathways through which ABI3 works should be further characterized.

  3. Fossil history and modern distribution of the genus Abies (Pinaceae)

    XIANG Xiaoguo; CAO Ming; ZHOU Zhekun


    The plants of the genus Abies are dominant and key species in the dark coniferous forest in the Northern Hemisphere.There are 52 species,one subspecies and 12 varieties in the genus Abies in the world.The history and modern distribution of Abies were discussed.The genus has three modern distribution centers:South Europe,North America and East Asia.These areas are also rich in fossil records.The vertical distribution regions of Abies are from sea level to an elevation of 4,700 m,concentrated in 1,000-2,000 m(15 species).In China,the genus distributes in 20 provinces,especially abundant in the Hengduan Mountains.Meanwhile,endemic and relic phenomena are obvious in this genus.There are seven relic species with both limited individuals and limited distributed regions.Based on the fossil records and the latest phylogenetic data,the following hypothesis was proposed:Abies originated from the middle and high altitudes of the Northern Hemisphere in the middle Cretaceous and it was dispersed southward in the Eocene due to global climate cooling down.The distribution of Abies was deeply influenced by geological events such as the uplift of Himalaya,the Alps,the Rocky Mountains,the occurrence of the Asian Monsoon as well as the Quaternary glaciers.Finally,the current distribution pattern appeared in the Quaternary.The genus Abies has a fossil history and modern distribution pattern similar to that of Cathaya and Pseudolarix.

  4. Psühhiaatrilise abi osutamise problemaatika Eestis / Mari Amos

    Amos, Mari


    ÜRO piinamise ning muu julma, ebainimliku või inimväärikust alandava kohtlemise ja karistamise vastasest konventsioonist, millega Eesti ühines 1. juunil 2002. Kaasaegne ja asjakohane psüühilise abi osutamise regulatsioon Eestis puudub

  5. The Arabidopsis CROWDED NUCLEI genes regulate seed germination by modulating degradation of ABI5 protein

    Wenming Zhao; Chunmei Guan; Jian Feng; Yan Liang; Ni Zhan; Jianru Zuo; Bo Ren


    In Arabidopsis, the phytohormone abscisic acid (ABA) plays a vital role in inhibiting seed germination and in post-germination seedling establishment. In the ABA signaling pathway, ABI5, a basic Leu zipper transcription factor, has important functions in the regulation of seed germination. ABI5 protein localizes in nuclear bodies, along with AFP, COP1, and SIZ1, and was degraded through the 26S proteasome pathway. However, the mechanisms of ABI5 nuclear body formation and ABI5 protein degradation remain obscure. In this study, we found that the Arabidopsis CROWDED NUCLEI (CRWN) proteins, predicted nuclear matrix proteins essential for maintenance of nuclear morphology, also participate in ABA-control ed seed germination by regulating the degradation of ABI5 protein. During seed germination, the crwn mutants are hypersensitive to ABA and have higher levels of ABI5 protein compared to wild type. Genetic analysis suggested that CRWNs act upstream of ABI5. The observation that CRWN3 colocalizes with ABI5 in nuclear bodies indicates that CRWNs might participate in ABI5 protein degrada-tion in nuclear bodies. Moreover, we revealed that the extreme C-terminal of CRWN3 protein is necessary for its function in the response to ABA in germination. Our results suggested important roles of CRWNs in ABI5 nuclear body organization and ABI5 protein degradation during seed germination.


    Veronique Seidel


    Full Text Available Three active compounds obtained from the aerial parts of Abies nobilis and Pinus sylvestris isolated by different chromatographic techniques. Their structures were identified by NMR (1H, 13C, COSY, HMBC spectroscopic and mass spectrometric data and established as catechin, dehydroabietic acid and Dihydroconiferyl alcohol. The isolated compounds were exhibited activity against blood stream form of parasite Trypanosoma brucei brucei (S 427.

  7. ABI4 mediates antagonistic effects of abscisic acid and gibberellins at transcript and protein levels.

    Shu, Kai; Chen, Qian; Wu, Yaorong; Liu, Ruijun; Zhang, Huawei; Wang, Pengfei; Li, Yanli; Wang, Shengfu; Tang, Sanyuan; Liu, Chunyan; Yang, Wenyu; Cao, Xiaofeng; Serino, Giovanna; Xie, Qi


    Abscisic acid (ABA) and gibberellins (GAs) are plant hormones which antagonistically mediate numerous physiological processes, and their optimal balance is essential for normal plant development. However, the molecular mechanism underlying ABA and GA antagonism still needs to be determined. Here, we report that ABA-INSENSITIVE 4 (ABI4) is a central factor in GA/ABA homeostasis and antagonism in post-germination stages. ABI4 overexpression in Arabidopsis (OE-ABI4) leads to developmental defects including a decrease in plant height and poor seed production. The transcription of a key ABA biosynthetic gene, NCED6, and of a key GA catabolic gene, GA2ox7, is significantly enhanced by ABI4 overexpression. ABI4 activates NCED6 and GA2ox7 transcription by directly binding to the promoters, and genetic analysis revealed that mutation in these two genes partially rescues the dwarf phenotype of ABI4 overexpressing plants. Consistently, ABI4 overexpressing seedlings have a lower GA/ABA ratio than the wild type. We further show that ABA induces GA2ox7 transcription while GA represses NCED6 expression in an ABI4-dependent manner; and that ABA stabilizes the ABI4 protein whereas GA promotes its degradation. Taken together, these results suggest that ABA and GA antagonize each other by oppositely acting on ABI4 transcript and protein levels.


    AJGH Kostermans


    Full Text Available Seven species of Burretwdendron are recognized, of which B. siamensis and B. yunnanensis are new to science. The distributional area of the genus covers Siam (one species, Yunnan (two species, Kweichow (one species;  Kwangsi (three species  and Tonkin (two species. B. tonkinensis is reduced to the synonymy of B. hsienmu. A key to the species is presented.

  9. Terpenoids with anti-inflammatory activity from Abies chensiensis.

    Zhao, Qian-Qian; Wang, Shu-Fang; Li, Ya; Song, Qiu-Yan; Gao, Kun


    The phytochemical investigation of Abies chensiensis led to the isolation and identification of nine new compounds including eight triterpenoids (1-8) and a new abietane-type diterpene (9), along with three known compounds (10-12). The absolute configuration of 9 was assigned by X-ray diffraction analysis. Compounds 1-11 were evaluated for the anti-inflammatory activity. Among the tested compounds, 1, 2, 5 and 6 exhibited potent inhibitory activity with IC50 values of 15.97, 18.73, 20.18 and 10.97μM, respectively.

  10. Henri Frankfort, Aby Warburg and “Mythopoeic Thought”

    Paul Taylor


    Full Text Available This is the text of a lunchtime lecture given at the Warburg Institute in the spring of 2004. It formed part of a series concerned with past Warburg scholars, and was devoted to the work of Henri Frankfort, Director of the Institute from 1949 to 1954. The first third of the lecture was a rapid account of his career; the remainder of the lecture was an analysis and criticism of the concept of ‘primitive thinking’ in the work of Frankfort and Aby Warburg.

  11. Aby Warburg, Images and Exhibitions. Aby Warburg, Bilderreihen und Ausstellungen edited by Uwe Fleckner and Isabelle Woldt, Akademie Verlag, 2012

    Matthew Rampley


    Full Text Available This article reviews the latest volume in the collected works of Aby Warburg published by Akademie Verlag. The volume consists of exhibitions and plates of images Warburg compiled to illustrate lectures in the period between 1925-1929. The review focuses on two key issues raised by the publication: the light it casts on the Mnemosyne Atlas Warburg was working on at the same time, and, in particular, how it helps shape perceptions of the broader intellectual direction of Warburg's thinking in the final half decade of his life.

  12. The complete chloroplast genome sequence of Abies nephrolepis (Pinaceae: Abietoideae

    Dong-Keun Yi


    Full Text Available The plant chloroplast (cp genome has maintained a relatively conserved structure and gene content throughout evolution. Cp genome sequences have been used widely for resolving evolutionary and phylogenetic issues at various taxonomic levels of plants. Here, we report the complete cp genome of Abies nephrolepis. The A. nephrolepis cp genome is 121,336 base pairs (bp in length including a pair of short inverted repeat regions (IRa and IRb of 139 bp each separated by a small single copy (SSC region of 54,323 bp (SSC and a large single copy region of 66,735 bp (LSC. It contains 114 genes, 68 of which are protein coding genes, 35 tRNA and four rRNA genes, six open reading frames, and one pseudogene. Seventeen repeat units and 64 simple sequence repeats (SSR have been detected in A. nephrolepis cp genome. Large IR sequences locate in 42-kb inversion points (1186 bp. The A. nephrolepis cp genome is identical to Abies koreana’s which is closely related to taxa. Pairwise comparison between two cp genomes revealed 140 polymorphic sites in each. Complete cp genome sequence of A. nephrolepis has a significant potential to provide information on the evolutionary pattern of Abietoideae and valuable data for development of DNA markers for easy identification and classification.

  13. Ankle-brachial index (ABI), abdominal aortic calcification (AAC), and coronary artery calcification (CAC): the Jackson heart study.

    Tullos, Bobby W; Sung, Jung Hye; Lee, Jae Eun; Criqui, Michael H; Mitchell, Marc E; Taylor, Herman A


    To examine the associations of peripheral atherosclerosis, assessed by the ABI at baseline with the extent of AAC and with CAC measured by MDCT at follow-up examination in the Jackson Heart Study cohort. Four categories of ABI: 1.40. Presence of CAC/AAC was defined as scoring above the 75th percentile among participants with non-zero CT calcium scores. We conducted multivariable log-binomial models for this analysis examining the relationship between ABI and the presence of CAC or AAC using normal ABI (1.0 ≤ ABI ≤ 1.39) as the reference group. We estimated prevalence ratios adjusted for age, smoking, HTN, DM, BMI, LDL, HDL, CRP, systolic and diastolic blood pressure, and use of lipid-lowering medication. There were 2,398 patients in this analysis (women: 65 %, average age 55 years). AAC scores were not significantly different between sex. CAC scores were significantly higher in males than females regardless of ABI groups. The prevalence of significant AAC was 1.7 times higher for ABI 1.40 compared to those with normal ABI. The prevalence of the significant CAC was higher for ABI CAC prevalence did not differ between subjects with ABI > 1.40 compared to those with normal ABI. Lower ABI was significantly associated with the extent of AAC and CAC in this cohort. ABI can provide clinicians with an inexpensive additional tool to assess vascular health and cardiovascular risk without exposing the patient to ionizing radiation.

  14. The lactococcal abortive infection protein AbiP is membrane-anchored and binds nucleic acids.

    Domingues, Susana; McGovern, Stephen; Plochocka, Danuta; Santos, Mário A; Ehrlich, S Dusko; Polard, Patrice; Chopin, Marie-Christine


    AbiP, a lactococcal abortive phage infection system, has previously been shown to arrest phage bIL66M1 DNA replication around 10 min after infection and to inhibit the switch off of phage early transcripts. We report here the functional characterization and implication in the abortive infection phenotype of two domains identified in the AbiP sequence. We show that AbiP is a protein anchored to the membrane by an N-terminal membrane-spanning domain. Our results further suggest that membrane localization may be required for the anti-phage activity of AbiP. The remainder of the protein, which contains a putative nucleic acid binding domain, is shown to be located on the cytosolic side. Purified AbiP is shown to bind nucleic acids with an approximately 10-fold preference for RNA relative to ssDNA. AbiP interaction with both ssDNA and RNA molecules occurs in a sequence-independent manner. We have analyzed the effect of substitutions of aromatic and basic residues on the surface of the putative binding fold. In vitro and in vivo studies of these AbiP derivatives indicate that the previously reported effects on phage development might be dependent on the nucleic acid binding activity displayed by the membrane-bound protein.

  15. Genome-Wide Analysis of the bZIP Gene Family Identifies Two ABI5-Like bZIP Transcription Factors, BrABI5a and BrABI5b, as Positive Modulators of ABA Signalling in Chinese Cabbage

    Hu, Xiaochen; Sun, Congcong; Li, Yanlin; Wang, Dandan; Wang, Qinhu; Pei, Guoliang; Zhang, Yanfeng; Guo, Aiguang; Zhao, Huixian; Lu, Haibin; Mu, Xiaoqian; Hu, Jingjiang; Zhou, Xiaona; Xie, Chang Gen


    bZIP (basic leucine zipper) transcription factors coordinate plant growth and development and control responses to environmental stimuli. The genome of Chinese cabbage (Brassica rapa) encodes 136 putative bZIP transcription factors. The bZIP transcription factors in Brassica rapa (BrbZIP) are classified into 10 subfamilies. Phylogenetic relationship analysis reveals that subfamily A consists of 23 BrbZIPs. Two BrbZIPs within subfamily A, Bra005287 and Bra017251, display high similarity to ABI5 (ABA Insensitive 5). Expression of subfamily A BrbZIPs, like BrABI5a (Bra005287/BrbZIP14) and BrABI5b (Bra017251/BrbZIP13), are significantly induced by the plant hormone ABA. Subcellular localization assay reveal that both BrABI5a and BrABI5b have a nuclear localization. BrABI5a and BrABI5b could directly stimulate ABA Responsive Element-driven HIS (a HIS3 reporter gene, which confers His prototrophy) or LUC (LUCIFERASE) expression in yeast and Arabidopsis protoplast. Deletion of the bZIP motif abolished BrABI5a and BrABI5b transcriptional activity. The ABA insensitive phenotype of Arabidopsis abi5-1 is completely suppressed in transgenic lines expressing BrABI5a or BrABI5b. Overall, these results suggest that ABI5 orthologs, BrABI5a and BrABI5b, have key roles in ABA signalling in Chinese cabbage. PMID:27414644

  16. 植物ABI4转录因子的研究进展%Research advances of transcription factor ABI4 in plants

    段星亮; 张晶; 宋晓东; 谢彦杰; 沈文飚


    Abscisic acid-insensitive 4 (ABI4) is a member of APETALA2/ethylene responsive factor (AP2/ERF) family, which was discovered and characterized to be an abscisic acid (ABA) signaling responsive tran-scription factor. Recent investigations illustrated that ABI4 is a multiple-faced regulatory factor in diverse cellu-lar processes. These biological events include seed germination and seedling establishment, plastid and/or mito-chondrial retrograde signaling, lipid metabolism, sugar signaling, and ABA responses etc. This paper summarized the latest research progress of ABI4 in terms of its structure characteristics, transcriptional regula-tion pattern, gene expression regulation, and signal transduction pathway.%Abscisic acid-insensitive 4(ABI4)属于APETALA2/ethylene responsive factor (AP2/ERF)类转录因子,它是在研究ABA信号转导途径中被发现和鉴定的。近年来, ABI4被认为是一种具有多种调节功能的转录因子,它参与了植物种子萌发和幼苗形态建成、质体/线粒体反向信号传递、脂类合成和分解、糖信号和ABA应答等诸多重要的生物学事件。本文综述了ABI4转录因子的结构特征、转录调控模式、基因表达调控和参与的信号转导功能等的最新研究进展。

  17. Portugal palus ELilt abi põlengutega võitlemiseks / Kajar Kase

    Kase, Kajar


    Sellel aastal on Portugali metsatulekahjudes hukkunud juba 15 inimest ja hetkel on kahjustatud 140 000 hektarit metsa. Appi on tõtanud Prantsusmaa, Saksamaa, Itaalia, Hispaania ja Hollandi helikopterid ja lennukid. Kaart: Abi Portugalile

  18. Future ofAbies pindrow in Swat district, northern Pakistan

    Kishwar Ali; Habib Ahmad; Nasrullah Khan; Stephen Jury


    Swat district is a biodiversity hub of Pakistan. The plant species, especially trees, in the Swat District are exposed to extinction threat from global climate change. Maximum entropy (MaxEnt) modelling of species distribution, using HADCM3 A2a global climate change scenario, pre-dicted a considerable change in the future distribution ofAbies pindrow (Royle ex D.Don) Royle. AUC (area under the curve)values of 0.972 and 0.983 were significant for the present and future distribution models of the species, respectively. It is clear that bioclimatic variables such as the mean temperature of the warmest quarter (bio_10) and the annual temperature range (bio_7) contribute significantly to the model and thus affect the predicted distribution and density of the species. The future model predicts that by the year 2080 population density will have decreased significantly. The highest density of the species is recorded in the eastern and western borders of the Valley in the areas of Sulatanr and Mankial. The changes in density and distribution of the species can have considerable impact, not only on the tree species itself, but on the associated subflora as well.

  19. Wood formation in Abies balsamea seedlings subjected to artificial defoliation.

    Rossi, Sergio; Simard, Sonia; Deslauriers, Annie; Morin, Hubert


    We determined the cambial sensitivity and quantified the anatomical differences in xylem of Abies balsamea (L.) Mill. seedlings subjected to artificial defoliation to simulate spruce budworm feeding. Defoliation was performed by removing two-thirds of needles of all current-year shoots for up to four consecutive growth cycles to account for inter- and intra-annual xylem formation. In Experiment 1, xylem development was studied from May to October 2005 in seedlings defoliated at the end of June. In Experiment 2, anatomical features of the xylem were measured along the tree rings formed in 2005 and 2006 during the four cycles of growth and defoliation. Control and defoliated seedlings showed similar patterns of cambial activity and timing of xylem differentiation, although fewer enlarging cells were observed in August to September in defoliated seedlings. Tree-ring widths were similar in control and defoliated seedlings, with thinner rings produced in the greenhouse in winter. No effect of defoliation on cell lumen area was observed, and effects on radial cell diameter and wall thickness were found only occasionally. The results indicate that the A. balsamea seedlings produced all the resources required to maintain stem growth during the four cycles of defoliation.

  20. ABySS-Explorer: visualizing genome sequence assemblies.

    Nielsen, Cydney B; Jackman, Shaun D; Birol, Inanç; Jones, Steven J M


    One bottleneck in large-scale genome sequencing projects is reconstructing the full genome sequence from the short subsequences produced by current technologies. The final stages of the genome assembly process inevitably require manual inspection of data inconsistencies and could be greatly aided by visualization. This paper presents our design decisions in translating key data features identified through discussions with analysts into a concise visual encoding. Current visualization tools in this domain focus on local sequence errors making high-level inspection of the assembly difficult if not impossible. We present a novel interactive graph display, ABySS-Explorer, that emphasizes the global assembly structure while also integrating salient data features such as sequence length. Our tool replaces manual and in some cases pen-and-paper based analysis tasks, and we discuss how user feedback was incorporated into iterative design refinements. Finally, we touch on applications of this representation not initially considered in our design phase, suggesting the generality of this encoding for DNA sequence data.

  1. Delphinella Shoot Blight on Abies lasiocarpa Provenances in Norway

    Venche Talgø


    Full Text Available Delphinella shoot blight (Delphinella abietis attacks true firs (Abies spp. in Europe and North America. Especially subalpine fir (A. lasiocarpa, one of the main Christmas tree species in Norway, is prone to the disease. The fungus kills current year needles, and in severe cases entire shoots. Dead needles become covered with black fruiting bodies, both pycnidia and pseudothecia. Delphinella shoot blight has mainly been a problem in humid, coastal regions in the northwestern part of Southern Norway, but, probably due to higher precipitation in inland regions during recent years, heavy attacks were found in 2011 in a field trial with 76 provenances of subalpine fir in Southeastern Norway. However, the amount of precipitation seemed less important once the disease had established in the field. Significant differences in susceptibility between provenances were observed. In general, the more bluish the foliage was, the healthier the trees appeared. The analysis of provenance means indicated that, at least for the southern range, the disease ratings were correlated with foliage color. This study also includes isolation, identification, a pathogenicity test, a seed test and electron microscopy of the wax layer on the needles. The fungus was identified based on the morphology of spores and by sequencing the Internal Transcribed Spacer (ITS regions of the ribosomal DNA. Koch’s postulates were fulfilled. The fungus was found present on newly harvested seeds and may therefore spread via international seed trade. When comparing the wax layers on green and blue needles, those of the latter were significantly thicker, a factor that may be involved in disease resistance.

  2. Phylogeography of SW Mediterranean firs: different European origins for the North African Abies species.

    Sánchez-Robles, Jose M; Balao, Francisco; Terrab, Anass; García-Castaño, Juan L; Ortiz, María A; Vela, Errol; Talavera, Salvador


    The current distribution of Western Mediterranean Abies species is a result of complex geodynamic processes and climatic oscillations that occurred in the past. Abies sect. Piceaster offers a good study model to explore how geo-climatic oscillations might have influenced its expansion and diversification on both sides of the W Mediterranean basin. We investigated the genetic variation within and among nine populations from five Abies species by molecular markers with high and low mutation rates and contrasting inheritance (AFLP and cpSSR). Analyses revealed the opening of the Strait of Gibraltar as an effective barrier against gene flow between the Southern Iberian (A. pinsapo) and North African (A. marocana and A. tazaotana) firs. The A. pinsapo populations in Spain and likewise those of the A. marocana - A. tazaotana population complex were not differentiated, and no evidence was found to distinguish A. tazaotana at the species level. Diversification of Abies across North Africa could occur by way of at least two vicariant events from Europe, in the west, giving rise to the A. marocana - A. tazaotana complex, and in the east, giving A. numidica. Secondary contacts among species from Abies sect. Piceaster (A. pinsapo and A. numidica), and with A. alba (Abies sect. Abies) are also indicated. However, there is a closer relationship between the Algerian fir (A. numidica) and the North Mediterranean widespread A. alba, than with the Moroccan firs (A. marocana and A. tazaotana) or the Southern Iberian (A. pinsapo). We also discuss the distribution range of these taxa in its paleogeological and paleoclimatic context, and propose that part of the modern geography of the South-Western Mediterranean firs might be traced back to the Tertiary.

  3. Analisis Perbandingan Database Jurnal Elektronik Emerald, ProQuest ABI/INFORM dan SpringerLink Bidang Manajemen dan Ekonomi

    Karyatin, Arianiansyah


    Penelitian ini dilakukan pada situs database jurnal elektronik Emerald, ProQuest ABI/INFORM dan SpringerLink. Tujuan penelitian ini adalah untuk mengetahui bagaimana konten (muatan) subjek kajian manajemen dan ekonomi pada database Emerald, ProQuest ABI/INFORM dan SpringerLink dan untuk mengetahui di dalam database manakah diantara database Emerald, ProQuest ABI/INFORM, dan Springerlink yang memuat bidang kajian manajemen dan ekonomi yang paling lengkap. Metode yang digunakan dalam pene...

  4. Ecological environment analysis of Abies holophylla plantations under different cutting systems


    Different types of plantations were observed in Baishilazi National Nature Reserve, Liaoning Province,for 34 a. The environmental quality of Abies holophylla plantations was analyzed under different cutting systems.Analysis factors included soil erosion rate, humification degree of litters, and water hold capacity. The surface soil loss of clear cutting area was 19 000 t·km-2·a-1 more than that of selective cutting area. The content of soil organic matter in board-leaved-Abies holophylla forest was 4.62% more than that in pure stand, and the water hold capacity of the mixed forest was 1.43 time of that of pure stand. The mixed forest of board-leaved--Abies holophylla by selective cutting can upgrade the ecological environment quality.

  5. Differential regulation of macropinocytosis by Abi1/Hssh3bp1 isoforms.

    Patrycja M Dubielecka

    Full Text Available BACKGROUND: Macropinocytosis, which is a constitutive cellular process of fluid and macromolecule uptake, is regulated by actin cytoskeleton rearrangements near the plasma membrane. Activation of Rac1, which is proposed to act upstream of the actin polymerization regulatory Wave 2 complex, has been found to correlate with enhanced macropinocytosis. One of the components of the Wave 2 complex is Abi1. Multiple, alternatively spliced isoforms of Abi1 are expressed in mammalian cells, but the functional significance of the various isoforms is unknown. PRINCIPAL FINDINGS: Here, using flow cytometric assay analysis for Alexa Fluor 647, we demonstrate that Abi1 isoforms 2 and 3 differentially regulate macropinocytosis. LNCaP cells expressing isoform 3 had increased macropinocytic uptake that correlated with enhanced cell spreading and higher Rac1 activation in comparison to cells expressing isoform 2. Isoform 2 expressing cells had decreased macropinocytic uptake, but demonstrated greater sensitivity to Rac1 activation. Moreover, more isoform 2 was localized within the cytoplasm in comparison to isoform 3, which was more associated with the plasma membrane. Activated Rac1 was found to specifically bind to a site in exon 10 of isoform 2 in vitro. Because of alternative mRNA splicing, exon 10 is absent from isoform 3, precluding similar binding of activated Rac1. Both isoforms, however, bound to inactive Rac1 through the same non-exon 10 site. Thus, Abi1 isoform 3-containing Wave 2 complex exhibited a differential binding to activated vs. inactive Rac1, whereas isoform 2-containing Wave 2 complex bound activated or inactive Rac1 comparably. CONCLUSION: Based on these observations, we postulate that Abi1 isoforms differentially regulate macropinocytosis as a consequence of their different relative affinities for activated Rac1 in Wave 2 complex. These findings also raise the possibility that isoform-specific roles occur in other Abi1 functions.

  6. Department of Defense (DOD) Automated Biometric Identification System (ABIS) Version 1.2: Initial Operational Test and Evaluation Report


    Director, Operational Test and Evaluation Department of Defense (DOD) Automated Biometric Identification System (ABIS) Version 1.2 Initial...Operational Test and Evaluation Report May 2015 This report on the Department of Defense (DOD) Automated Biometric Identification System...COVERED - 4. TITLE AND SUBTITLE Department of Defense (DOD) Automated Biometric Identification System (ABIS) Version 1.2 Initial Operational Test

  7. Identification and Characterization of the Phage Gene sav, Involved in Sensitivity to the Lactococcal Abortive Infection Mechanism AbiV

    Haaber, Jakob Brandt Borup; Rousseau, G. M.; Hammer, Karin;


    Lactococcus lactis phage mutants that are insensitive to the recently characterized abortive infection mechanism AbiV were isolated and analyzed in an effort to elucidate factors involved in the sensitivity to AbiV. Whole-genome sequencing of the phage mutants p2.1 and p2.2 revealed mutations...

  8. Nuclear microsatellite primers for the endangered relict fir, Abies pinsapo (Pinaceae) and cross-amplification in related Mediterranean Species.

    Sánchez-Robles, Jose M; Balao, Francisco; García-Castaño, Juan L; Terrab, Anass; Navarro-Sampedro, Laura; Talavera, Salvador


    Twelve nuclear microsatellite primers (nSSR) were developed for the endangered species Abies pinsapo Boiss. to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454) data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other Abies species from the Mediterranean Basin, and most of the loci showed higher polymorphisms in the Mediterranean species than in A. pinsapo. These microsatellite markers provide tools for conservation genetic studies in Abies pinsapo as well other Abies species from the Mediterranean Basin.

  9. Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae and Cross-Amplification in Related Mediterranean Species

    Laura Navarro-Sampedro


    Full Text Available Twelve nuclear microsatellite primers (nSSR were developed for the endangered species Abies pinsapo Boiss. to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454 data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other Abies species from the Mediterranean Basin, and most of the loci showed higher polymorphisms in the Mediterranean species than in A. pinsapo. These microsatellite markers provide tools for conservation genetic studies in Abies pinsapo as well other Abies species from the Mediterranean Basin.


    Yéo N'Gana


    Full Text Available This work is a commented translation of the tale La force du serment by the Ivorian writer Amon d’Aby. We aimed at analyzing through/by the translation both figures of style particularly framed by an authoritative imaginary requiring that reflections be made on the translation of space, and linguistic and cultural elements found within the tale.

  11. The Classical Classroom: Enhancing Learning for Pupils with Acquired Brain Injury (ABI)

    Rees, Sian A.; Skidmore, David


    This paper seeks to draw parallels between different approaches to classroom instruction and two contrasting musical styles and to examine how pupils with Acquired Brain Injuries (ABI) might fare in each. A polyphonic classroom is defined as one where an awareness of multiple layers of meaning are encouraged to enhance the learning opportunities,…

  12. [Genetic control of Silver fir isozymes (Abies alba Mill.) of the Ukrainian Carpathian Mountains].

    Korshikov, I I; Morozova, N N; Pirko, Ia V


    Genetic control of GOT, GDH, DIA, MDH, ME, SOD, FDH, ADH, ACP, LAP enzymes has been studied in the seed megagametophytes of Silver fir (Abies alba Mill.) from four natural populations of the Ukrainian Carpathian mountains. The distinct electrophoretic division has been obtained for the 21 loci products. The analysis of allele segregation in the heterozygous trees confirms monogenic inheritance of the revealed variants.

  13. Iraagi peaminister tänas Eestit abi eest / Toivo Tootsen

    Tootsen, Toivo, 1943-


    Välisminister Urmas Paeti kohtumisest Iraagi peaministri Nuri al-Malikiga, mille käigus tänas peaminister Eestit osutatud abi eest ning rõhutas Iraagi iseseisvumisprotsessi tähtsust, mille saavutamisel on oluline roll ka koalitsioonivägedel

  14. Pakistan vajab abi - kas maailma tõesti ei huvita? / Urmas Jaagant

    Suurkask, Heiki, 1972-


    Pakistan saab igal aastal suurt rahvusvahelist abi. Mitmed riigid on üleujutustes Pakistani toetanud nüüdki kümnete miljonite dollaritega, kuid riikide tahe annetada on erinev, sest mitte iga abidollarit ei suunata Pakistanis sinna, kus seda tegelikult vajatakse

  15. Cytokinin profiles in the conifer tree Abies nordmanniana

    Rasmussen, Hanne Nina; Veierskov, Bjarke; Hansen-Møller, Jens;


    Abstract  Conifer trees are routinely manipulated hormonally to increase flowering, branching, or adjust crown shape for production purposes. This survey of internal cytokinin levels provides a background for such treatments in Abies nordmanniana, a tree of great economic interest. Reference poin...

  16. Tree mortality patterns following prescribed fire for Pinus and Abies across the southwestern United States

    van Mantgem, Philip J.; Nesmith, Jonathan C. B.; Keifer, MaryBeth; Brooks, Matthew


    The reintroduction of fire to historically fire-prone forests has been repeatedly shown to reduce understory fuels and promote resistance to high severity fire. However, there is concern that prescribed fire may also have unintended consequences, such as high rates of mortality for large trees and fire-tolerant Pinus species. To test this possibility we evaluated mortality patterns for two common genera in the western US, Pinus and Abies, using observations from a national-scale prescribed fire effects monitoring program. Our results show that mortality rates of trees >50 DBH were similar for Pinus (4.6% yr-1) and Abies (4.0% yr-1) 5 years following prescribed fires across seven sites in the southwestern US. In contrast, mortality rates of trees >50 cm DBH differed between Pinus (5.7% yr-1) and Abies (9.0% yr-1). Models of post-fire mortality probabilities suggested statistically significant differences between the genera (after including differences in bark thickness), but accounting for these differences resulted in only small improvements in model classification. Our results do not suggest unusually high post-fire mortality for large trees or for Pinus relative to the other common co-occurring genus, Abies, following prescribed fire in the southwestern US.

  17. ABI4 regulates primary seed dormancy by regulating the biogenesis of abscisic acid and gibberellins in arabidopsis.

    Shu, Kai; Zhang, Huawei; Wang, Shengfu; Chen, Mingluan; Wu, Yaorong; Tang, Sanyuan; Liu, Chunyan; Feng, Yuqi; Cao, Xiaofeng; Xie, Qi


    Seed dormancy is an important economic trait for agricultural production. Abscisic acid (ABA) and Gibberellins (GA) are the primary factors that regulate the transition from dormancy to germination, and they regulate this process antagonistically. The detailed regulatory mechanism involving crosstalk between ABA and GA, which underlies seed dormancy, requires further elucidation. Here, we report that ABI4 positively regulates primary seed dormancy, while negatively regulating cotyledon greening, by mediating the biogenesis of ABA and GA. Seeds of the Arabidopsis abi4 mutant that were subjected to short-term storage (one or two weeks) germinated significantly more quickly than Wild-Type (WT), and abi4 cotyledons greened markedly more quickly than WT, while the rates of germination and greening were comparable when the seeds were subjected to longer-term storage (six months). The ABA content of dry abi4 seeds was remarkably lower than that of WT, but the amounts were comparable after stratification. Consistently, the GA level of abi4 seeds was increased compared to WT. Further analysis showed that abi4 was resistant to treatment with paclobutrazol (PAC), a GA biosynthesis inhibitor, during germination, while OE-ABI4 was sensitive to PAC, and exogenous GA rescued the delayed germination phenotype of OE-ABI4. Analysis by qRT-PCR showed that the expression of genes involved in ABA and GA metabolism in dry and germinating seeds corresponded to hormonal measurements. Moreover, chromatin immunoprecipitation qPCR (ChIP-qPCR) and transient expression analysis showed that ABI4 repressed CYP707A1 and CYP707A2 expression by directly binding to those promoters, and the ABI4 binding elements are essential for this repression. Accordingly, further genetic analysis showed that abi4 recovered the delayed germination phenotype of cyp707a1 and cyp707a2 and further, rescued the non-germinating phenotype of ga1-t. Taken together, this study suggests that ABI4 is a key factor that

  18. Activation of mRNA translation by phage protein and low temperature: the case of Lactococcus lactis abortive infection system AbiD1

    Ehrlich S Dusko


    Full Text Available Abstract Background Abortive infection (Abi mechanisms comprise numerous strategies developed by bacteria to avoid being killed by bacteriophage (phage. Escherichia coli Abis are considered as mediators of programmed cell death, which is induced by infecting phage. Abis were also proposed to be stress response elements, but no environmental activation signals have yet been identified. Abis are widespread in Lactococcus lactis, but regulation of their expression remains an open question. We previously showed that development of AbiD1 abortive infection against phage bIL66 depends on orf1, which is expressed in mid-infection. However, molecular basis for this activation remains unclear. Results In non-infected AbiD1+ cells, specific abiD1 mRNA is unstable and present in low amounts. It does not increase during abortive infection of sensitive phage. Protein synthesis directed by the abiD1 translation initiation region is also inefficient. The presence of the phage orf1 gene, but not its mutant AbiD1R allele, strongly increases abiD1 translation efficiency. Interestingly, cell growth at low temperature also activates translation of abiD1 mRNA and consequently the AbiD1 phenotype, and occurs independently of phage infection. There is no synergism between the two abiD1 inducers. Purified Orf1 protein binds mRNAs containing a secondary structure motif, identified within the translation initiation regions of abiD1, the mid-infection phage bIL66 M-operon, and the L. lactis osmC gene. Conclusion Expression of the abiD1 gene and consequently AbiD1 phenotype is specifically translationally activated by the phage Orf1 protein. The loss of ability to activate translation of abiD1 mRNA determines the molecular basis for phage resistance to AbiD1. We show for the first time that temperature downshift also activates abortive infection by activation of abiD1 mRNA translation.

  19. Metabolite changes in conifer buds and needles during forced bud break in Norway spruce (Picea abies and European silver fir (Abies alba

    Priyanka eDhuli


    Full Text Available Environmental changes such as early spring and warm spells induce bud burst and photosynthetic processes in cold-acclimated coniferous trees and consequently, cellular metabolism in overwintering needles and buds. The purpose of the study was to examine metabolism in conifers under forced deacclimation (artificially induced spring by exposing shoots of Picea abies (boreal species and Abies alba (temperate species to a greenhouse environment (22°C, 16/8 h D/N cycle over a nine week period. Each week, we scored bud opening and collected samples for GC/MS–based metabolite profiling. We detected a total of 169 assigned metabolites and 80 identified metabolites, comprising compounds such as mono- and disaccharides, Krebs cycle acids, amino acids, polyols, phenolics and phosphorylated structures. Untargeted multivariate statistical analysis based on PCA and cluster analysis segregated samples by species, tissue type, and stage of tissue deacclimations. Similar patterns of metabolic regulation in both species were observed in buds (amino acids, Krebs cycle acids and needles (hexoses, pentoses, and Krebs cycle acids. Based on correlation of bud opening score with compound levels, distinct metabolites could be associated with bud and shoot development, including amino acids, sugars and acids with known osmolyte function, and secondary metabolites. This study has shed light on how elevated temperature affects metabolism in buds and needles of conifer species during the deacclimation phase, and contributes to the discussion about how phenological characters in conifers may respond to future global warming.

  20. Abies semenovii B. Fedtsch. at the Peter the Great Botanical Garden

    Tkachenko Kirill


    Full Text Available Abies semenovii B. Fedtsch. (Pinaceae is an extremely rare flora species of the Central Asia (Kirghizia; it has been cultivated at the Peter the Great Botanical Garden of the Komarov Botanical Institute of the Russian Academy of Sciences (RAS since 1949, where it was first introduced into general cultivation. Since 2000, upon reaching the age of 43 years, the seed reproduction of the plants is being marked. An X-ray test proved seeds, collected in 2014, to be filled and full. In spring 2015, first time in the 67 years of cultivating this specie in St. Petersburg area, first young crops were received. Abies semenovii – a cold hard and decorative tree – has to be introduced into the gardening of St. Petersburg and shall be promoted into the Karelia and further to the northern regions of the European part of the Russian Federation.

  1. Analysis of the Phialocephala subalpina Transcriptome during Colonization of Its Host Plant Picea abies.

    Vanessa Reininger

    Full Text Available Phialocephala subalpina belongs to the Phialocephala fortinii s.l.-Acepphala applanata species complex (PAC forming one of the major groups belonging to the dark septate endophytes (DSE. Depending on the strain, PAC was shown to form neutral to pathogenic associations with its host plant Picea abies. To understand PACs lifestyle we investigated the effect of presence/absence of Picea abies on the transcriptome of strain 6_70_1.PAC strain 6_70_1 was grown in liquid Pachlewski media either induced by its host plant Picea abies or without host plant as a control. Mycelia were harvested in a time course (1, 2, 3, 4, 7, 11, 18 days with and without induction by the host plant and the fungal transcriptome revealed by Illumina sequencing. Differential gene expression analysis over the time course comparing control and treatment at each time point using the 'edgeR glm approach' and a gene enrichment analysis using GO categories were performed.The three main functional groups within differentially expressed genes were 'metabolism', 'transport' and 'cell rescue, defense and virulence'. Additionally, genes especially involved in iron metabolism could be detected by gene set enrichment analysis.In conclusion, we found PAC strain 6_70_1 to be metabolically very active during colonization of its host plant Picea abies. A major shift in functional groups over the time course of this experiment could not be observed but GO categories which were found to be enriched showed different emphasis depending in the day post induction.

  2. [Genetic control of isozymes in European spruces (Picea abies (L) Karst) of the Ukrainian Carpathian mountains].

    Privalikhin, S N; Korshikov, I I; Pirko, N N; Velikorid'ko, T I; Pirko, Ia V


    Genetical control of the enzymes GOT, GDH, DIA, MDH, SOD, FDH, ADH, ACP and LAP has been studied in nine natural Carpathian populations of Norway spruce (Picea abies (L.) Karst.) using polyacrylamide gel elecrophoresis and analysis of isozyme variability in 346 trees. Seventy one allel products of 20 gene loci have been clearly established. Segregation analysis of the revealed allele variants confirms their monogenic inheritance.

  3. Recherche de lignées aromatiques d’Abies par hybridation somatique

    Fauconnier, Marie-Laure


    Créer une lignée d’Abies aromatique qui associera les propriétés de croissance reconnues à l’espèce A. nordmanniana aux propriétés aromatiques d’A. balsamea, en utilisant des lignées embryogènes capables de régénérer des plantes entières.

  4. Mycocoenology in Abies alba Miller woods of central-southern Tuscany (Italy)

    Angela Laganà; Elena Salerni; Carla Barluzzi; Claudia Perini; Vincenzo de Dominicis


    Numerous reports indicate that fir woods in central and northern Europe have recently been damaged by increasing pollution. It has been demonstrated that fungi can be good bioindicators of forest health status. In polluted areas the production of fruit bodies generally declines and the fungal biodiversity, especially of symbiotic species, is reduced. Here we report the results of a survey of the fungal and plant communities in woods of Abies alba Miller in central-southern Tuscany, already st...

  5. Final Scientific/Technical report for "ABI8: Prototype of a novel signaling factor"

    Finkelstein, Ruth R. [Univ. of California, Santa Barbara, CA (United States)


    The Arabidopsis thaliana ABSCISIC ACID-INSENSITIVE8 locus encodes a highly conserved plant-specific protein that mediates abscisic acid (ABA) and sugar responses essential for growth. Although initial database comparisons revealed no domains of predictable function, it has recently been re-annotated as a member of the Glycosyltransferase family A. However, this function has not been demonstrated experimentally and no specific substrates have been identified. Mutations affecting ABI8 are near-lethal due to pleiotropic yet specific effects including altered ABA signaling, sugar transport, cell wall synthesis, root meristem maintenance, vascular patterning, and male sterility. Because the predicted sequence initially provided no clues, we used a guilt by association strategy to address function of this protein by determining its subcellular localization and identifying interacting proteins. Our studies showed that ABI8 is localized to the endomembrane system and may interact with proteins implicated in Golgi trafficking, lignification, and stress signaling. We found that the root meristem arrest reflects decreased auxin accumulation and resulting decreases in regulators required for meristem identity, all of which can be rescued by added glucose. Further studies showed that this glucose-dependence reflects reduced glucose uptake as well as the decreased expression of sugar-mobilizing enzymes. This work suggests that ABI8 may regulate trafficking of membrane proteins such as auxin transporters and cellulose synthase, but this hypothesis has not yet been tested. The altered gene expression is likely to be a secondary or later effect of this pleiotropic mutation.

  6. Genetic Variation among 11 Abies concolor Populations Based on Allozyme Analysis

    Zhang Jin-feng; Li Hui; Dong Jian-sheng; Wang Jun-hui


    In order to obtain information on the genetic structure of Abies concolor and the genetic variation among 11 populations introduced from America to China, allozyme analysis based on starch gel electrophoresis technology was used. 24 loci of 10allozyme systems were mensurated, and the genetic structure and genetic diversity of the 11 populations of A. concolor evaluated.The results show that the genetic variation among is significant, and the genetic variation within A. concolor populations is more important. In contrast with other conifers, the variation of A. concolor is above the average level of conifers, and higher than the same level ofAbies. The percentage of polymorphic loci (P) was 62.5%, the number of alleles per locus (A) 2.08, the number of effective alleles per locus (Ae) was 1.37, the expected heterozygosity (H) 0.204, and the Shannon information index (I) 0.351 7. There is a short genetic distance (D=0.061) and a low gene flow (Nm=0.839 4) among the 11 introduced populations of A. concolor with high genetic variation. The genetic differentiation coefficient (Gst) was 0.229 5, which is higher than that of the mean in Abies or Pinus.

  7. An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.

    Stefan Liebau

    Full Text Available BACKGROUND: The stabilization or regulated reorganization of the actin cytoskeleton is essential for cellular structure and function. Recently, we could show that the activation of the SK3-channel that represents the predominant SK-channel in neural stem cells, leads to a rapid local outgrowth of long filopodial processes. This observation indicates that the rearrangement of the actin based cytoskeleton via membrane bound SK3-channels might selectively be controlled in defined micro compartments of the cell. PRINCIPAL FINDINGS: We found two important proteins for cytoskeletal rearrangement, the Abelson interacting protein 1, Abi-1 and the neural Wiskott Aldrich Syndrome Protein, nWASP, to be in complex with SK3- channels in neural stem cells (NSCs. Moreover, this interaction is also found in spines and postsynaptic compartments of developing primary hippocampal neurons and regulates neurite outgrowth during early phases of differentiation. Overexpression of the proteins or pharmacological activation of SK3 channels induces obvious structural changes in NSCs and hippocampal neurons. In both neuronal cell systems SK3 channels and nWASP act synergistic by strongly inducing filopodial outgrowth while Abi-1 behaves antagonistic to its interaction partners. CONCLUSIONS: Our results give good evidence for a functional interplay of a trimeric complex that transforms incoming signals via SK3-channel activation into the local rearrangement of the cytoskeleton in early steps of neuronal differentiation involving nWASP and Abi-1 actin binding proteins.

  8. Lactococcal Abortive Infection Protein AbiV Interacts Directly with the Phage Protein SaV and Prevents Translation of Phage Proteins

    Haaber, Jakob Brandt Borup; Samson, J.E.; Labrie, S.J.;


    RNAs and proteins suggested that AbiV blocks the activation of late gene transcription, probably by a general inhibition of translation. Using size exclusion chromatography coupled with on-line static light scattering and refractometry, as well as fluorescence quenching experiments, we also demonstrated that both......AbiV is an abortive infection protein that inhibits the lytic cycle of several virulent phages infecting Lactococcus lactis, while a mutation in the phage gene sav confers insensitivity to AbiV. In this study, we have further characterized the effects of the bacterial AbiV and its interaction...... with the phage p2 protein SaV. First, we showed that during phage infection of lactococcal AbiV(+) cells, AbiV rapidly inhibited protein synthesis. Among early phage transcripts, sav gene transcription was slightly inhibited while the SaV protein could not be detected. Analyses of other phage p2 m...

  9. ABI3 mediates expression of the peroxiredoxin antioxidant AtPER1 gene and induction by oxidative stress.

    Haslekås, Camilla; Grini, Paul E; Nordgard, Silje H; Thorstensen, Tage; Viken, Marte K; Nygaard, Vigdis; Aalen, Reidunn B


    The peroxiredoxin antioxidant gene AtPER1 has been considered to be specifically expressed in the embryo and aleurone layer during maturation and desiccation stages of development, and in the mature seed, typically for late embryogenesis-abundant (lea) transcripts. In the abscisic acid-insensitive abi3-1 mutant, the AtPER1 transcript level is strongly reduced, suggesting ABI3 to be a prime regulator of AtPER1. We have studied the expression pattern and regulation of AtPER1 with a series of nine promoter::GUS constructs with deletions and/or mutations in putative regulatory elements. Arabidopsis lines harbouring these constructs revealed AtPER1 promoter activity in the endosperm, especially the chalazal cyst, already when the embryo is in the late globular stage, in the embryo from the late torpedo stage, and in distinct cells of unfertilized and fertilized ovules. Early expression seems to be dependent on a putative antioxidant-responsive promoter element (ARE), while from the bent cotyledon stage endosperm and embryo expression is dependent on an ABA-responsive element (ABRE) likely to bind ABI5. The shortest promoter fragment (113 bp), devoid of ARE, ABRE and without an intact RY/Sph element thought to bind ABI3 did not drive GUS expression. The AtPER1::GUS construct also revealed expression in cotyledons, meristems and stem branching points. In general, seed and vegetative expression coincided with the expression pattern of ABI3. In plants ectopically expressing ABI3, AtPER1::GUS expression was found in true leaves, and AtPER1 could be induced by exogenous ABA and oxidative stress (H2O2 and hydroquinone). ABI3-mediated oxidative stress induction was dependent on the presence of an intact ARE element.

  10. Identification of Salt Stress Biomarkers in Romanian Carpathian Populations of Picea abies (L. Karst.

    Sorin T Schiop

    Full Text Available The Norway spruce (Picea abies, the most important tree species in European forests, is relatively sensitive to salt and does not grow in natural saline environments. Yet many trees are actually exposed to salt stress due to the common practice of de-icing of mountain roads in winter, using large amounts of NaCl. To help develop strategies for an appropriate use of reproductive seed material on reforestation sites, ensuring better chances of seedling survival in salt-affected areas, we have studied the responses of young spruce seedlings to salt treatments. The specific aim of the work was to identify the optimal salt stress biomarkers in Picea abies, using as experimental material seedlings obtained by germination of seeds with origin in seven populations from the Romanian Carpathian Mountains. These responses included general, conserved reactions such as the accumulation of ions and different osmolytes in the seedlings needles, reduction in photosynthetic pigments levels, or activation of antioxidant systems. Although changes in the contents of different compounds involved in these reactions can be associated to the degree of stress affecting the plants, we propose that the (decreasing levels of total phenolics or total carotenoids and the (increasing levels of Na+ or K+ ions in Picea abies needles, should be considered as the most reliable and useful biomarkers for salt stress in this species. They all show very high correlation with the intensity of salt stress, independently of the genetic background of the seeds parental population, and relatively easy, quantitative assays are available to determine their concentrations, requiring simple equipment and little amount of plant material.

  11. Identification of Salt Stress Biomarkers in Romanian Carpathian Populations of Picea abies (L.) Karst.

    Schiop, Sorin T; Al Hassan, Mohamad; Sestras, Adriana F; Boscaiu, Monica; Sestras, Radu E; Vicente, Oscar


    The Norway spruce (Picea abies), the most important tree species in European forests, is relatively sensitive to salt and does not grow in natural saline environments. Yet many trees are actually exposed to salt stress due to the common practice of de-icing of mountain roads in winter, using large amounts of NaCl. To help develop strategies for an appropriate use of reproductive seed material on reforestation sites, ensuring better chances of seedling survival in salt-affected areas, we have studied the responses of young spruce seedlings to salt treatments. The specific aim of the work was to identify the optimal salt stress biomarkers in Picea abies, using as experimental material seedlings obtained by germination of seeds with origin in seven populations from the Romanian Carpathian Mountains. These responses included general, conserved reactions such as the accumulation of ions and different osmolytes in the seedlings needles, reduction in photosynthetic pigments levels, or activation of antioxidant systems. Although changes in the contents of different compounds involved in these reactions can be associated to the degree of stress affecting the plants, we propose that the (decreasing) levels of total phenolics or total carotenoids and the (increasing) levels of Na+ or K+ ions in Picea abies needles, should be considered as the most reliable and useful biomarkers for salt stress in this species. They all show very high correlation with the intensity of salt stress, independently of the genetic background of the seeds parental population, and relatively easy, quantitative assays are available to determine their concentrations, requiring simple equipment and little amount of plant material.

  12. Effects of irrigation, fertilization and drought on the occurrence of Lophodermium piceae in Picea abies needles

    Lehtijaervi, Asko; Barklund, Pia [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Mycology and Pathology


    Effects of irrigation, drought and fertilization on the frequency of the fungal endophyte Lophodermium piceae in green needles was assessed in a 30-year-old experimental stand of Picea abies in southern Sweden. Frequencies of needles with L. piceae were lower in irrigation and ammonium sulphate fertilization treatments than in the control. Drought treatment frequencies were similar to the control. Needles were susceptible to colonization for at least 3 years; colonization increased with needle age. The results indicate that the increased availability of water to the root system as well as ammonium sulphate fertilization indirectly delays colonization of needles by L. piceae 21 refs, 3 figs, 2 tabs

  13. Electron probe microanalysis of fluorine in polluted needle sections of Abies alba

    Garrec, J.P.; Lhoste, A.M.


    After fumigation with hydrogen fluoride, sections of needles of Abies alba were studied by electron probe microanalysis. The levels of fluoride vary in different tissues: most fluoride was found in the spongy mesophyll and the palisade tissues as a function of the distance from the cells of entry and the paths of translocation of fluoride. These results support the view that the differential responses of various tissues of a leaf to fumigation with HF depend on the localization of the tissues rather than on a specific response by a particular tissue to fluoride. 15 references, 6 figures.

  14. The afterlife of antiquity and modern art: Aby Warburg on Manet

    Dimitrios Latsis


    Full Text Available Aby Warburg’s manuscript on Édouard Manet – unpublished during his lifetime and presented here for the first time in English – constitutes one of his rare, substantial commentaries on nineteenth century art. Using “Le Déjeuner sur l’herbe” as a departure point, Warburg proceeds from his customary meticulous investigation of the central motif’s “visual archeology,” to a larger reflection on the evolution of the representation of nature in art and the image of antiquity that modernity has created for itself.

  15. ELi abi ettevõtjale lükkub osaliselt edasi / Silva Männik

    Männik, Silva, 1974-


    Eesti ettevõtjatele lubatud Euroopa Liidu tõukefondide raha taotlemine Ettevõtluse Arendamise Sihtasutuses viibib majandusministeeriumis vastuvõtmata määruste tõttu. Diagrammid: Struktuurifondidest saadava abi kõrval peab Eesti liikmemaksu tasuma; EAS plaanis jagada tänavu ligi 800 miljonit krooni struktuurifondide raha. Nimekiri: Põhiosa euroabist läheb töötajate arengusse ja transporti. 5 prioriteeti. Vt. samas: Raha venimine kõige hullem. AS-i E-Arsenal juht Jüri Tümanok rahade viibimisest

  16. Decline of sacred fir (Abies religiosa) in a forest park south of Mexico City.

    Alvarado R, D; De Bauer, L I; Galindo A, J


    Decline of sacred fir (Abies religiosa) trees in the high elevation forest park, Desierto de los Leones, located south of Mexico City, is described. Trees located in the windward zone (exposed to air masses from Mexico City) were the most severely affected, especially trees at the distal ends of ravines. Examination of tree growth rings indicated decreases in ring widths for the past 30 years. Polluted air from Mexico City may be an important causal factor in fir decline. Drought, due to excessive removal of soil water, insects, mites and pathogens, and poor forest management are possible contributing and interactive factors in fir decline.


    Al Makin


    Full Text Available This article questions the domination of the prophethood of Muhammad in the narrative of the seventh century of the Arabian Peninsula presented by both Muslim and Western scholars. There were many other claimants to prophethood, who are ignored in Muslim and Western sources. In this vein, this article deals with Umayya ibn Abi Salt, a poet who claimed prophethood. Umayya’s short biography, collections of his poems (diwan and , and examples of his poems are discussed. Keywords: prophethood, qur’an, Umayya, revelation, sira, diwan.

  18. E3B1/ABI-1 Isoforms Are Down-Regulated in Cancers of Human Gastrointestinal Tract

    Rafia A. Baba


    Full Text Available The expression of E3B1/ABI-1 protein and its role in cancer progression and prognosis are largely unknown in the majority of solid tumors. In this study, we examined the expression pattern of E3B1/ABI-1 protein in histologically confirmed cases of esophageal (squamous cell carcinoma and adenocarcinoma, gastro-esophageal junction, colorectal cancers and corresponding normal tissues freshly resected from a cohort of 135 patients, by Western Blotting and Immunofluorescence Staining. The protein is present in its phosphorylated form in cells and tissues. Depending on the extent of phosphorylation it is either present in hyper-phosphorylated (M. Wt. 72 kDa form or in hypo-phosphorylated form (M. Wt. 68 kDa and 65 kDa. A thorough analysis revealed that expression of E3B1/ABI-1 protein is significantly decreased in esophageal, gastro-esophageal junction and colorectal carcinomas irrespective of age, gender, dietary and smoking habits of the patients. The decrease in expression of E3B1/ABI-1 was consistently observed for all the three isoforms. However, the decrease in the expression of isoforms varied with different forms of cancers. Down-regulation of E3B1/ABI-1 expression in human carcinomas may play a critical role in tumor progression and in determining disease prognosis.

  19. In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter: possible role of this interaction in the expression of seed dormancy.

    Cantoro, Renata; Crocco, Carlos Daniel; Benech-Arnold, Roberto Luis; Rodríguez, María Verónica


    The precise adjustment of the timing of dormancy release according to final grain usage is still a challenge for many cereal crops. Grain sorghum [Sorghum bicolor (L.) Moench] shows wide intraspecific variability in dormancy level and susceptibility to pre-harvest sprouting (PHS). Both embryo sensitivity to abscisic acid (ABA) and gibberellin (GA) metabolism play an important role in the expression of dormancy of the developing sorghum grain. In previous works, it was shown that, simultaneously with a greater embryo sensitivity to ABA and higher expression of SbABA-INSENSITIVE 4 (SbABI4) and SbABA-INSENSITIVE 5 (SbABI5), dormant grains accumulate less active GA4 due to a more active GA catabolism. In this work, it is demonstrated that the ABA signalling components SbABI4 and SbABI5 interact in vitro with a fragment of the SbGA 2-OXIDASE 3 (SbGA2ox3) promoter containing an ABA-responsive complex (ABRC). Both transcription factors were able to bind the promoter, although not simultaneously, suggesting that they might compete for the same cis-acting regulatory sequences. A biological role for these interactions in the expression of dormancy of sorghum grains is proposed: either SbABI4 and/or SbABI5 activate transcription of the SbGA2ox3 gene in vivo and promote SbGA2ox3 protein accumulation; this would result in active degradation of GA4, thus preventing germination of dormant grains. A comparative analysis of the 5'-regulatory region of GA2oxs from both monocots and dicots is also presented; conservation of the ABRC in closely related GA2oxs from Brachypodium distachyon and rice suggest that these species might share the same regulatory mechanism as proposed for grain sorghum.

  20. Kinetic modeling of the ultrasound-assisted extraction of polyphenols from Picea abies bark.

    Lazar, Liliana; Talmaciu, Adina Iulia; Volf, Irina; Popa, Valentin I


    In this paper, the kinetics of polyphenols extraction from spruce bark (Picea abies) under ultrasounds action was investigated. Studies were performed in order to express the effect of some specific parameters (as: ultrasounds, surface contact between solvent and solid, extraction time and temperature) on the total phenolic content (TPC). Experiments were performed in the presence and absence of ultrasounds, using different contact surfaces between solvent and solid, for times from 5 to 75min and temperatures of 318, 323 and 333K. All these factors have a positive influence on the process, enhancing the extraction rate by recovering higher amounts of polyphenols. The process takes place in two stages: a fast one in the first 20-30min (first stage), followed by a slow one approaching to an equilibrium concentration after 40min (second stage). In these conditions, the second-order kinetic model was successfully developed for describing the mechanism of ultrasound-assisted extraction of polyphenols from P. abies bark. Based on this model, values of second-order extraction rate constant (k), initial extraction rate (h), saturation concentration (Cs) and activation energy (Ea) could be predicted. Model validation was done by plotting experimental and predicted values of TPC's, revealing a very good correlation between the obtained data (R(2)>0.98).

  1. Outcomes after Cognitive Perceptual Motor Retraining (CPM of Patients with Acquired Brain Injury (ABI

    Kara Christy


    Full Text Available Background: Remediation of deficits is one approach used by occupational therapists in the treatment of clients with acquired brain injury (ABI. This retrospective study examined outcomes after participation in Cognitive Perceptual Motor Retraining (CPM of clients with ABI and identified demographic and injury characteristics of clients that were associated with outcomes. CPM was delivered as part of the standard treatment and was not designed for research purposes. Method: A retrospective review of 59 client records was completed. CPM evaluation test scores, demographic information, and injury characteristics were extracted from the records. Results: There were moderate improvements in CPM test scores and good discharge outcomes for most clients. Discharge to home with independent status was associated with mild to moderate traumatic brain injury severity and being married. Longer time since injury and having a concurrent psychiatric diagnosis were associated with longer duration of CPM. Conclusion: This preliminary study demonstrates positive therapy outcomes after CPM. Recommendations were made for future research and considerations in the use of CPM. These include the need for addressing concurrent needs, such as psychological issues and repeated re-evaluations, to determine when clients have met maximum remediation and thereby minimizing cost.

  2. Simulation of GOES-R ABI aerosol radiances using WRF-CMAQ: a case study approach

    Christopher, S. A.


    In anticipation of the upcoming GOES-R launch we simulate visible and near-infrared reflectances of the Advanced Baseline Imager (ABI) for cases of high aerosol loading containing regional haze and smoke over the eastern United States. The simulations are performed using the Weather Research and Forecasting (WRF), Sparse Matrix Operator Kernel Emissions (SMOKE), and Community Multiscale Air Quality (CMAQ) models. Geostationary, satellite-derived, biomass-burning emissions are also included as an input to CMAQ. Using the CMAQ aerosol concentrations and Mie calculations, radiance is computed from the discrete ordinate atmospheric radiative transfer model. We present detailed methods for deriving aerosol extinction from WRF and CMAQ outputs. Our results show that the model simulations create a realistic set of reflectances in various aerosol scenarios. The simulated reflectances provide distinct spectral features of aerosols which are then compared to data from the Moderate Resolution Imaging Spectroradiometer (MODIS). We also present a simple technique to synthesize green band reflectance (which will not be available on the ABI), using the model-simulated blue and red band reflectance. This study is an example of the use of air quality modeling in improving products and techniques for Earth-observing missions.

  3. Simulation of GOES-R ABI aerosol radiances using WRF-CMAQ: a case study approach

    S. A. Christopher


    Full Text Available The primary focus of this paper is to simulate visible and near-infrared reflectances of the GOES-R Advanced Baseline Imager (ABI for cases of high aerosol loading containing regional haze and smoke over the eastern United States. The simulations are performed using the Weather Research and Forecasting (WRF, Sparse Matrix Operator Kernel Emissions (SMOKE, and Community Multiscale Air Quality (CMAQ models. Geostationary satellite-derived biomass burning emissions are also included as an input to CMAQ. Using the CMAQ aerosol concentrations and Mie calculations, radiance is computed from the discrete ordinate atmospheric radiative transfer model. We present detailed methods for deriving aerosol extinction from WRF and CMAQ outputs. Our results show that the model simulations create a realistic set of reflectance in various aerosol scenarios. The simulated reflectance provides distinct spectral features of aerosols which is then compared to data from the Moderate Resolution Imaging Spectroradiometer (MODIS. We also present a simple technique to synthesize green band reflectance (which will not be available on the ABI, using the model-simulated blue and red band reflectance. This study is an example of the use of air quality modeling in improving products and techniques for Earth observing missions.

  4. A weeding-duration model for Abies sachalinensis plantations in Hokkaido, northern Japan

    Masahiko Nakagawa


    I developed a weeding-duration model for Sakhalin fir (Abies sachalinensis (Fr.Schmidt) Masters) plantations that employs a generalized linear model.The number of years following planting that weeding is necessary is the response variable,and elevation,slope steepness,maximum snow depth,annual precipitation,geology,soil,site index,slope aspect,and vegetation type are explanatory variables.Among the explanatory variables,geology,soil,slope aspect,and vegetation type are categorical data.A Poisson distribution is assumed for the response variable,with a log-link function.Elevation,slope steepness,maximum snow depth,annual precipitation,site index,and vegetation type had a significant effect on weeding duration.Among the eight models with the smallest Akaike information criterion (AIC),I chose the model with no multicollinearity among the explanatory variables.The weeding-duration model includes site index,maximum snow depth,slope steepness (angle)and vegetation type as explanatory variables; elevation and annual precipitation were not included in the selected model because of multicollinearity with maximum snow depth.This model is useful for cost-benefit analyses of afforestation or reforestation with Abies sachalinensis.

  5. Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers

    Kovačević Dragan


    Full Text Available Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%. The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus. [Projekat Ministarstva nauke Republike Srbije, br.173029

  6. The hydraulic conductivity of the xylem in conifer needles (Picea abies and Pinus mugo).

    Charra-Vaskou, Katline; Mayr, Stefan


    Main resistances of the plant water transport system are situated in leaves. In contrast to angiosperm leaves, knowledge of conifer needle hydraulics and of the partitioning of resistances within needles is poor. A new technique was developed which enabled flow-meter measurements through needles embedded in paraffin and thus quantification of the specific hydraulic conductivity (K(s)) of the needle xylem. In Picea abies, xylem K(s) of needle and axes as well as in needles of different age were compared. In Pinus mugo, resistance partitioning within needles was estimated by measurements of xylem K(s) and leaf conductance (K(leaf), measured via 'rehydration kinetics'). Mean K(s) in P. abies needles was 3.5×10(-4) m(2) s(-1) MPa(-1) with a decrease in older needles, and over all similar to K(s) of corresponding axes xylem. In needles of P. mugo, K(s) was 0.9×10(-4) m(2) s(-1) MPa(-1), and 24% of total needle resistance was situated in the xylem. The results indicate species-specific differences in the hydraulic efficiency of conifer needle xylem. The vascular section of the water transport system is a minor but relevant resistance in needles.

  7. Characterization of variable EST SSR markers for Norway spruce (Picea abies L.

    Spiess Nadine


    Full Text Available Abstract Background Norway spruce is widely distributed across Europe and the predominant tree of the Alpine region. Fast growth and the fact that timber can be harvested cost-effectively in relatively young populations define its status as one of the economically most important tree species of Northern Europe. In this study, EST derived simple sequence repeat (SSR markers were developed for the assessment of putative functional diversity in Austrian Norway spruce stands. Results SSR sequences were identified by analyzing 14,022 publicly available EST sequences. Tri-nucleotide repeat motifs were most abundant in the data set followed by penta- and hexa-nucleotide repeats. Specific primer pairs were designed for sixty loci. Among these, 27 displayed polymorphism in a testing population of 16 P. abies individuals sampled across Austria and in an additional screening population of 96 P. abies individuals from two geographically distinct Austrian populations. Allele numbers per locus ranged from two to 17 with observed heterozygosity ranging from 0.075 to 0.99. Conclusions We have characterized variable EST SSR markers for Norway spruce detected in expressed genes. Due to their moderate to high degree of variability in the two tested screening populations, these newly developed SSR markers are well suited for the analysis of stress related functional variation present in Norway spruce populations.

  8. ABI domain-containing proteins contribute to surface protein display and cell division in Staphylococcus aureus.

    Frankel, Matthew B; Wojcik, Brandon M; DeDent, Andrea C; Missiakas, Dominique M; Schneewind, Olaf


    The human pathogen Staphylococcus aureus requires cell wall anchored surface proteins to cause disease. During cell division, surface proteins with YSIRK signal peptides are secreted into the cross-wall, a layer of newly synthesized peptidoglycan between separating daughter cells. The molecular determinants for the trafficking of surface proteins are, however, still unknown. We screened mutants with non-redundant transposon insertions by fluorescence-activated cell sorting for reduced deposition of protein A (SpA) into the staphylococcal envelope. Three mutants, each of which harboured transposon insertions in genes for transmembrane proteins, displayed greatly reduced envelope abundance of SpA and surface proteins with YSIRK signal peptides. Characterization of the corresponding mutations identified three transmembrane proteins with abortive infectivity (ABI) domains, elements first described in lactococci for their role in phage exclusion. Mutations in genes for ABI domain proteins, designated spdA, spdB and spdC (surface protein display), diminish the expression of surface proteins with YSIRK signal peptides, but not of precursor proteins with conventional signal peptides. spdA, spdB and spdC mutants display an increase in the thickness of cross-walls and in the relative abundance of staphylococci with cross-walls, suggesting that spd mutations may represent a possible link between staphylococcal cell division and protein secretion.

  9. Modelling initial mortality of Abies religiosa in a crown fire in Mexico

    Salomé Temiño-Villota


    Full Text Available Aim of study: The objectives of this work were to determine which morphological and fire severity variables may help explain the mortality of adult Abies religiosa (Kunth Schltdl. & Cham., to model the probability of this species after being affected by crown fire, and to obtain more elements to classify the sacred fir in terms of fire resistance. This type of studies are relevant to estimate the impact of crown fires on the climax forests that forms this species.Area of study: The burned forest was located in the southern Mexico City, borough.Material and methods: Morphological variables and fire severity indicators were collected for 335 Abies religiosa trees burned by a mixed severity fire. Logistic regression was used to analyze data and develop models that best explained tree mortality.Main results: Survival was 26.9%. The models for height (p≤0.0001, diameter at breast height (p=0.0082, crown length (p≤0.0001 and crown base height (p≤0.0001 were significant, with a negative relationship between each one of these variables and probability of mortality. The significant severity variables were lethal scorch height (p≤0.0001 and crown kill (p≤ 0.0001, which have a direct relationship with probability of mortality.Highlights: This species is moderately fire-resistant. Crown kill ≥ 70% markedly increases mortality. Silvicultural activities such as pruning, thinning and fuel management can reduce the risk of crown fires.

  10. The Andean Biotic Index (ABI): revised tolerance to pollution values for macroinvertebrate families and index performance evaluation.

    Ríos-Touma, Blanca; Acosta, Raúl; Prat, Narcís


    Score-based biotic indices are widely used to evaluate the water quality of streams and rivers. Few adaptations of these indices have been done for South America because there is a lack of knowledge on macroinvertebrate taxonomy, distribution and tolerance to pollution in the region. Several areas in the Andes are densely populated and there is need for methods to assess the impact of increasing human pressures on aquatic ecosystems. Considering the unique ecological and geographical features of the Andes, macroinvertebrate indices used in other regions must be adapted with caution. Here we present a review of the literature on macroinvertebrate distribution and tolerance to pollution in Andean areas above 2,000 masl. Using these data, we propose an Andean Biotic Index (ABI), which is based on the BMWP index. In general, ABI includes fewer macroinvertebrate families than in other regions of the world where the BMWP index has been applied because altitude restricts the distribution of several families. Our review shows that in the high Andes, the tolerance of several macroinvertebrate families to pollution differs from those reported in other areas. We tested the ABI index in two basins in Ecuador and Peru, and compared it to other BMWP adaptations using the reference condition approach. The ABI index is extremely useful for detecting the general impairment of rivers but class quality boundaries should be defined independently for each basin because reference conditions may be different. The ABI is widely used in Ecuador and Peru, with high correlations with land-use pressures in several studies. The ABI index is an integral part of the new multimetric index designed for high Andean streams (IMEERA).

  11. Mechanisms of Abnormal Growth Regulation in Prostatic Adenocarcinoma Using Abi1/Hssh3bp1 Conditional Knockout Mouse Model


    growth factor-BB human was from Sigma, (P3201); Alexa Fluor 594 Phalloidin was from Molecular Probes (Invitrogen, 41A2 -2). Abi1 immunostaining in...Invitrogen - Molecular Probes, A11001) and Alexa Fluor 594 Phalloidin (Invitrogen -Molecular Probes, 41A2 -2). Antibodies to Wave complex

  12. Koolieeliku hirmudega toimetulek kui oma probleemidega toimetuleku õppimine ja lähedaste abi selles / Merle Taimalu

    Taimalu, Merle


    Eestis ja Soomes 1993-1994.a. läbi viidud ühisuurimusest laste turvalisuse kohta, kus üheks eesmärgiks oli välja selgitada, kuidas koolieelikud oma hirmudega toime tulevad ja kuivõrd nad saavad ja kasutavad lähedaste täiskasvanute abi


    Ioana Ieremia


    Full Text Available The paper present the influence of ascorbic acid upon the mitotic division of Larix decidua Mill ssp. carpatica and Picea abies (L. Karst. The treatment is applied of two variants, germinated seed in ascorbic acid (variantAand germinated seeds in disttilate water, than treated with ascorbic acid in 3 concentrations (variant B.

  14. Nuevos genes reguladores de la tolerancia a estrés abiótico en Arabidopsis.



    Martínez Macías, F. (2015). Nuevos genes reguladores de la tolerancia a estrés abiótico en Arabidopsis [Tesis doctoral no publicada]. Universitat Politècnica de València. doi:10.4995/Thesis/10251/48560. TESIS

  15. An experimental study on the effects of exhaust gas on spruce (Picea abies L. Karst.)

    Hautala, E.L.; Holopainen, J.; Kaerenlampi, L. [Kuopio Univ. (Finland). Dept. of Ecology and Environmental Science; Surakka, J.; Ruuskanen, J. [Kuopio Univ. (Finland). Dept. of Environmental Sciences


    Motor vehicle exhausts are significant contributors to air pollution. Besides fine particles and inorganic gases, like CO, SO{sub 2} and NO{sub x}, exhaust gas contains a large group of aromatic hydrocarbon compounds, many of which are phytotoxic. In field studies, exhausts are found to have both direct and indirect harmful effects on roadside plants. However, only few experimental studies have been made about the effects of exhaust gas emissions on coniferous trees. The aim of this study was to survey the effects of exhausts on spruce (Picea abies L. Karst.) in standardized conditions. The concentrations of major exhaust gas components in the chamber atmosphere were detected simultaneously. The effects of exhaust on epistomatal waxes of first-year spruce needles are described. (author)

  16. Tree ring variability and climate response of Abies spectabilis along an elevation gradient in Mustang, Nepal

    Kharal, D.K.; Meilby, Henrik; Rayamajhi, S.


    In mountainous areas including the Himalayas, tree lines are expected to advance to higher altitudes due to global climate change affecting the distribution and growth of plant species. This study aimed at identifying the tree ring variability of Abies spectabilis (D. Don) and its response...... to the climate along an elevation gradient in the high Himalayas of central Nepal. Tree core samples were collected from four sites in Mustang district. All sites were located in the same valley and exposed to similar weather conditions. Out of 232 samples collected from the sites, Titi lower (2700 m), Titi...... upper (2900 m), Pangukhark (3100 m) and Lete upper (3300 m), 44, 40, 39 and 41 series were successfully cross-dated and ring width chronologies including 168, 79, 138 and 156 years previous to 2012 were developed, respectively. Statistically significant differences in average annual radial growth were...

  17. Climatic significance of stable carbon isotope in tree rings of Abies spectabibis in southeastern Tibet

    LIU Xiaohong; Qin Dahe; SHAO Xuemei; CHEN Tuo; REN Jiawen


    The annually cross-dated stable carbon isotope of tree-ring α-cellulose of Abies spectabibis collected from the southeastern Tibetan Plateau is used to examine its relationship with climatic parameters. The residual △13C series in treerings is constructed after removing the effects of age trend and rising CO2. We found a close relationship between △13C in tree rings and the relative humidity of September-November of the previous year measured at the nearby Nyingchi Meteorological Station, albeit a strong "lagged effect". Thus we developed a transfer function to reconstruct the autumn relative humidity for the Nyingchi region, which explained 37.9% of the total variance (p < 0.001). Our results suggest a high frequency and moderate amplitude variance of the relative humidity before 1800, and the variance reversed afterwards.

  18. Exploratory assessment of groundwater vulnerability to pollution in Abi, southeastern Nigeria, using geophysical and geological techniques.

    Akpan, Anthony E; Ebong, Ebong D; Emeka, Chimezie N


    The geophysical-based integrated electrical conductivity (IEC) and the groundwater hydraulic confinement-overlying strata-depth to water table (GOD) techniques were used to assess vulnerability levels of aquifers and the extent of aquifer protection in Abi, Nigeria. The IEC indices was generated from constrained one dimensional (1D) inversion of vertical electrical sounding (VES) and two dimensional (2D) electrical resistivity tomography (ERT) data, acquired randomly in the area. The GOD indices were sourced from existing geologic data within the area. Results showed that IEC values vary from 2.0 S in the strongly protected areas. The GOD indices vary from groundwater resources in the area need to be properly managed for sustainability and such management practices have been suggested.

  19. Geographical Variation in the Composition of Abies bornmuelleriana Mattf. Needle Oils

    Güneş Uçar


    Full Text Available This study is to investigate the effect of the geographic variation on the composition of needle oils of Abies bornmuelleriana endemic to Turkey. Samples were taken from the stands distanced up to 500 km from each other. By means of GC-MS/FID analyses, it was found that main compounds making up about 90 percent of the needle essential oils exhibit notable systematic variation depending on the longitude where the trees grow. Among most occurring monoterpenes, the percentages of a -pinene and camphene tend to increase in west–east direction. To compensate these increments a decrease was observed in mainly the three monoterpenes, β-pinene, β-phellandrene and limonene.

  20. Lignin distribution in waterlogged archaeological Picea abies (L.) Karst degraded by erosion bacteria

    Pedersen, Nanna Bjerregaard; Schmitt, Uwe Schmitt; Koch, Gerald


    degraded S2 showed a varied lignin distribution as evidenced by the different local UV-absorbance intensities. However, evaluation of UV-absorbance line spectra of RM revealed no change in conjugation of the aromatic ring system. Presence of RM with both very low and very high lignin absorbances showed......The lignin distribution in poles of waterlogged archaeological Picea abies (L.) Karst, which was decayed by erosion bacteria (EB) under anoxic conditions for approximately 400 years, was topochemically identified by transmission electron microscopy (TEM) and high resolution UV...... evidence for disassembly of lignin during degradation combined with aggregation of lignin fragments and physical movement of these fractions. In contrast to TEM analysis, locally decreasing lignin content was found by UMSP in CML regions....

  1. Growing trees on completed sanitary landfills. [Nyssa sylvatica, Picea abies, Ginkgo biloba

    Leone, I.A.; Gilman, E.F.; Flower, F.B.


    A 10-year old completed landfill in New Jersey consisting of 9 m (depth) of refuse covered with 15-25 cm of soil was cleared of debris and vegetation and covered with 30 cm of subsoil and 15-25 cm of topsoil. Nineteen coniferous and broadleaved species were planted on the landfill and on a control site in 1975, and trees were maintained and growth and condition monitored over 4 years. On the basis of shoot length and stem area increase, the most successful of the surviving trees were Nyssa sylvatica, Picea abies and Ginkgo biloba, in decreasing order of tolerance. Tolerance of landfill conditions appeared to be greatest in those species with low water requirements, a slow growth rate, high acid tolerance and a shallow root system. (Refs. 11).

  2. Sobre el caso Aby Warburg : La cura por el símbolo

    Erbetta, Anahí E.; Morete, Amalia


    En el presente trabajo nos ocuparemos del caso del historiador de arte alemán Aby Warburg (1886-1929), reconocido por el estudio de la historia cultural a través de las artes visuales. Retiene nuestra atención desde una perspectiva psicoanalítica el desencadenamiento de una psicosis clínica a la edad de 52 años. Es internado en la clínica Bellevue, en Kreuzlingen, dirigida por el Dr. Binswanger, desde el año 1921 al año 1924. Si bien el motivo de internación reside en el riesgo de vida en que...

  3. Mycocoenology in Abies alba Miller woods of central-southern Tuscany (Italy

    Angela Laganà


    Full Text Available Numerous reports indicate that fir woods in central and northern Europe have recently been damaged by increasing pollution. It has been demonstrated that fungi can be good bioindicators of forest health status. In polluted areas the production of fruit bodies generally declines and the fungal biodiversity, especially of symbiotic species, is reduced. Here we report the results of a survey of the fungal and plant communities in woods of Abies alba Miller in central-southern Tuscany, already studied in the past. Certain changes were observed in the study areas, but they seem more likely to be due to other factors than pollution, such as the weather conditions, the age and natural evolution of the forests towards their climax.

  4. The work of Ernst H. Gombrich on the Aby M. Warburg fragments

    Katia Mazzucco


    Full Text Available On January 1, 1936, Ernst Gombrich arrived in London from Austria. His task was to reorganize the last of Warburg’s fragments and, in particular, to revise what was left of the incomplete mnemosyne atlas. The goal of this study and inventory was to collate Warburg’s uncompleted studies with the purpose of putting together an English publication of his collected work. In 1970, the only result of this publishing endeavour was included in the pages of the famous Aby Warburg. An intellectual biography. The history of the intellectual, as well as critical, relationship between Gombrich and Warburg is, then, a posthumous one that was started on British soil. Further marking this distance are certain material and non-material aspects of Gombrich’s work in London, including his relationship with other warburgian scholars.

  5. Annotation and Re-Sequencing of Genes from De Novo Transcriptome Assembly of Abies alba (Pinaceae

    Anna M. Roschanski


    Full Text Available Premise of the study: We present a protocol for the annotation of transcriptome sequence data and the identification of candidate genes therein using the example of the nonmodel conifer Abies alba. Methods and Results: A normalized cDNA library was built from an A. alba seedling. The sequencing on a 454 platform yielded more than 1.5 million reads that were de novo assembled into 25 149 contigs. Two complementary approaches were applied to annotate gene fragments that code for (1 well-known proteins and (2 proteins that are potentially adaptively relevant. Primer development and testing yielded 88 amplicons that could successfully be resequenced from genomic DNA. Conclusions: The annotation workflow offers an efficient way to identify potential adaptively relevant genes from the large quantity of transcriptome sequence data. The primer set presented should be prioritized for single-nucleotide polymorphism detection in adaptively relevant genes in A. alba.

  6. Tobacco exposure and diabetes-related autoantibodies in children: results from the ABIS study.

    Johansson, Annakarin; Hermansson, Göran; Ludvigsson, Johnny


    Passive smoking has decreased in recent years ("increased hygiene"). Less environmental tobacco smoke (ETS) gives increased hygiene that, if the hygiene hypothesis is true, in turn might give more autoimmune diseases. The presence of auto antibodies is considered to be an early indicator of type 1 diabetes (T1D). Because tobacco exposure may influence the immune system, we analyzed the relation between passive smoking and development of autoantibodies. A subsample (n= 8794) of the children in the ABIS study was used for this analysis. The parents answered questionnaires on smoking from pregnancy and onwards, and blood samples from the children aged 2.5-3 years were analyzed for GADA and IA-2A. Results showed that there was no significant difference in the prevalence of GADA or IA-2A (>95 percentile) between tobacco-exposed and nonexposed children. It was concluded that passive smoking does not seem to influence development of diabetes-related autoantibodies early in life.

  7. Genetic diversity of Norway spruce [Picea abies (L. Karst.] in Romanian Carpathians

    Raul Gheorghe Radu


    Full Text Available The genetic diversity of Romanian most important coniferous tree species, the Norway spruce, was estimated by means of allozyme markers. A total of 695 adult trees sampled from eleven populations grouped in six mountainous areas in the Romanian Carpathians were analyzed. In three metapopulations (Maramureş, Postăvar and Parâng, to evaluate the influence of altitudinal gradient on genetic diversity, samples were collected from populations located at high and low altitude. At other location (ApuseniMountains we compared the narrow-crown biotype (Picea abies var. columnaris and the pyramidal crown biotype (Picea abies var. pyramidalis and explored the genetic structure of peat bog ecotype. By analyzing 7 enzyme systems and 12 enzyme coding loci, a total of 38 allelic variants have been detected. The mean value of polymorphic loci for the six sites was 86.1%, ranging between 83.3% and 91.7% and the mean expected heterozygosity was 0.115, resulting in a moderate level of genetic diversity. The highest genetic diversity (He = 0.134 was found in the narrow-crown spruce population. Apuseni metapopulation showed the highest genetic diversity (He = 0.125, being the most valuable for conservation of genetic resources. The small value of fixation index (FST = 0.009 indicates a low genetic differentiation between the six sites and AMOVA test revealed a very high level of genetic diversity within population (99%. Comparative analysis of genetic parameters showed small differences between high and low altitude populations at each site, probably due to the neutral character of the markers analyzed and the effect of gene flow between gradiental populations.

  8. Regulation of actin cytoskeleton architecture by Eps8 and Abi1

    Miller Jeffrey R


    Full Text Available Abstract Background The actin cytoskeleton participates in many fundamental processes including the regulation of cell shape, motility, and adhesion. The remodeling of the actin cytoskeleton is dependent on actin binding proteins, which organize actin filaments into specific structures that allow them to perform various specialized functions. The Eps8 family of proteins is implicated in the regulation of actin cytoskeleton remodeling during cell migration, yet the precise mechanism by which Eps8 regulates actin organization and remodeling remains elusive. Results Here, we show that Eps8 promotes the assembly of actin rich filopodia-like structures and actin cables in cultured mammalian cells and Xenopus embryos, respectively. The morphology of actin structures induced by Eps8 was modulated by interactions with Abi1, which stimulated formation of actin cables in cultured cells and star-like structures in Xenopus. The actin stars observed in Xenopus animal cap cells assembled at the apical surface of epithelial cells in a Rac-independent manner and their formation was accompanied by recruitment of N-WASP, suggesting that the Eps8/Abi1 complex is capable of regulating the localization and/or activity of actin nucleators. We also found that Eps8 recruits Dishevelled to the plasma membrane and actin filaments suggesting that Eps8 might participate in non-canonical Wnt/Polarity signaling. Consistent with this idea, mis-expression of Eps8 in dorsal regions of Xenopus embryos resulted in gastrulation defects. Conclusion Together, these results suggest that Eps8 plays multiple roles in modulating actin filament organization, possibly through its interaction with distinct sets of actin regulatory complexes. Furthermore, the finding that Eps8 interacts with Dsh and induced gastrulation defects provides evidence that Eps8 might participate in non-canonical Wnt signaling to control cell movements during vertebrate development.

  9. Throughfall nutrients in a degraded indigenous Fagus orientalis forest and a Picea abies plantation in the of North of Iran

    Parisa Abbasian


    Full Text Available Aim of study: The objective of this study was to compare the quantity and quality of TF (throughfall in an indigenous, but degraded, stand of Fagus orientalis and Picea abies plantation.Area of study: Forests of Kelar-Dasht region located in Mazandaran province, northern Iran.Material and Methods: TF measured by twenty collectors that were distributed randomly underneath each stand. For 21 storms sampled in 2012 (August-December and 2013 (April-June, we analyzed pH, EC, Ca2+, Mg2+, K+, NO3-, and P of  gross rainfall (GR and TF.Main results: Cumulative interception (I for F. orientalis and P. abies were 114.2 mm and 194.8 mm of the total GR, respectively. The amount of K+ (13.4 mg L-1 and Ca2+ (0.9 mg L-1 were higher (for both elements, p = 0.001 in the TF of P. abies compared to those of F. orientalis (6.8 and 0.5, mg L-1, respectively and GR (3.2 and 0.37 mg L-1, respectively. Conversely, mean P concentration was doubled (p = 0.022 in the TF of F. orientalis (11.1 mg L-1 compared to GR (5.8 mg L-1.Research highlights: P. abies plantations may provide a solution for reforestation of degraded F. orientalis forests of northern Iran, yet how P. abies plantations differentially affect the quality and quantity of rainfall reaching subcanopy soils (TF compared to F. orientalis is unknown. Understanding the connection between hydrological processes and nutrient cycling in forest ecosystems is crucial for choosing the appropriate species to rehabilitate the degraded indigenous forests with nonindigenous species.  Keywords: concentration; hydrological process; interception; reforestation.

  10. Genetic and environmental characterization of Abies alba Mill. populations at its western rear edge

    Sancho-Knapik, D.


    Full Text Available The genetic structure of ten Abies alba populations at the western rear edge in the Spanish Pyrenees was characterized and compared with two German populations, looking for the role of climatic factors in the fir decline. Growth, defoliation, aridity and cumulative summer water deficit summer were also characterized. Spanish populations show a lower genetic diversity and a high genetic differentiation than German ones, mainly established by an East (higher diversity-West (lower diversity gradient. The three defoliated populations are the western ones, with higher summer aridity. Contrastingly, the southern population (Guara shows climatic variables close to western sites but without defoliation and with a higher genetic diversity, indicating a possible adaptation to the sub-Mediterranean conditions. Silver fir in the Spanish Pyrenees constitutes a “stable” rear edge because of their isolation, small sized and small genetic diversity. Western Pyrenean sites subjected to dryer conditions and presenting lower genetic diversity are prone to drought-induced mortality in the context of global warming.Se ha caracterizado la estructura genética de diez poblaciones de Abies alba en la retaguardia occidental de su distribución en el Pirineo español en comparación con dos poblaciones de Alemania, buscando la influencia de factores climáticos en el decaimiento del abeto. También se caracterizó crecimiento, defoliación, aridez y déficit acumulado de agua durante el verano. Las poblaciones españolas mostraron una menor diversidad genética y una mayor diferenciación genética que las poblaciones alemanas, establecida principalmente a través de un eje Este (mayor diversidad-Oeste (menor diversidad. Las tres poblaciones con defoliación son las del suroeste, con mayor aridez estival. Por el contrario, la población más meridional (Guara muestra variables climáticas cercanas a estas poblaciones del suroeste pero sin defoliación y con mayor

  11. Regulation of somatic embryo development in Norway spruce (Picea abies). A molecular approach to the characterization of specific developmental stages

    Sabala, I. [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Genetics


    Embryo development is a complex process involving a set of strictly regulated events. The regulation of these events is poorly understood especially during the early stages of embryo development. Somatic embryos go through the same developmental stages as zygotic embryos making them an ideal model system for studying the regulation of embryo development. We have used embryogenic cultures of Picea abies to study some aspects of the regulation of embryo development in gymnosperms. The bottle neck during somatic embryogenesis is the switch from the proliferation stage to the maturation stage. This switch is initiated by giving somatic embryos a maturation treatment i.e. the embryos are treated with abscisic acid (ABA). Somatic embryos which respond to ABA by forming mature somatic embryos were stimulated to secret a 70 kDa protein, AF70. The af70 gene was isolated and characterised. The expression of the af70 gene was constitutive in embryos but was highly ABA-induced in seedlings. Moreover, expression of this gene was stimulated during cold acclimation of Picea abies seedlings. A full length Picea abies cDNA clone Pa18, encoding a protein with the characteristics of plant lipid transfer proteins (LTPs), was isolated and characterised. The Pa18 gene is constitutively expressed in embryogenic cultures of Picea abies representing different stages of development as well as in nonembryogenic callus and seedlings. In situ hybridization showed that Pa18 gene is expressed in all embryonic cells of proliferating somatic embryos but the expression of the gene in mature somatic and zygotic embryos is restricted to the outer cell layer. Southern blot analysis at different stringencies was consistent with a single gene. An alteration in expression of Pa18 causes disturbance in the formation of the proper outer cell layer in the maturing somatic embryos. In addition to its influence on embryo development the Pa18 gene product also inhibits growth of Agrobacterium tumefaciens 195

  12. La Huella del montaje Aby Warburg; Aldo van Eyck, Jerzy Grotowski : recorridos a partir del Atlas Mnemosyne

    Konstantopoulou, Dimitra


    Premi extraordinari doctorat curs 2010-2011, àmbit d’Arquitectura, Urbanisme i Edificació The thesis consists in constructing an itinerary through three fields "culture of science, architecture, theatre" and three authors Aby Warburg, Aldo van Eyck, Jerzy Grotowski, on the basis of the idea of the montage. Its methodological reference in spite of the inadequacy of any literal reference to the term "method" in this case' is Warburg's Atlas Mnemosyne; on the one hand, since it constitutes it...

  13. Peroxidase activity, soluble proteins and chlorophyll content in spruce (Picea abies L. Karst.) needles affected by cement dust

    Cesar, Vera; Lepeduš, Hrvoje


    The correlation between the peroxidase activity, chlorophyll and soluble protein content as well as the changes in vascular bundle structure in Norway spruce (Picea abies L. Karst.) needles affected by cement dust were studied. In spite of the absence of any yellowing symptoms, a significantly lower chlorophyll content was measured in spruce needles affected by cement dust. Observed sieve cells distortions in needle samples indicated that spruce trees grown near the cement factory were Mg def...

  14. Conserved function of core clock proteins in the gymnosperm Norway spruce (Picea abies L. Karst).

    Karlgren, Anna; Gyllenstrand, Niclas; Källman, Thomas; Lagercrantz, Ulf


    From studies of the circadian clock in the plant model species Arabidopsis (Arabidopsis thaliana), a number of important properties and components have emerged. These include the genes CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), GIGANTEA (GI), ZEITLUPE (ZTL) and TIMING OF CAB EXPRESSION 1 (TOC1 also known as PSEUDO-RESPONSE REGULATOR 1 (PRR1)) that via gene expression feedback loops participate in the circadian clock. Here, we present results from ectopic expression of four Norway spruce (Picea abies) putative homologs (PaCCA1, PaGI, PaZTL and PaPRR1) in Arabidopsis, their flowering time, circadian period length, red light response phenotypes and their effect on endogenous clock genes were assessed. For PaCCA1-ox and PaZTL-ox the results were consistent with Arabidopsis lines overexpressing the corresponding Arabidopsis genes. For PaGI consistent results were obtained when expressed in the gi2 mutant, while PaGI and PaPRR1 expressed in wild type did not display the expected phenotypes. These results suggest that protein function of PaCCA1, PaGI and PaZTL are at least partly conserved compared to Arabidopsis homologs, however further studies are needed to reveal the protein function of PaPRR1. Our data suggest that components of the three-loop network typical of the circadian clock in angiosperms were present before the split of gymnosperms and angiosperms.

  15. Improvement of grafting procedures for the ornamental species: II. Abies concolor [(Gord. & Glend. Lindl

    Ioan Blada


    Full Text Available The achieved results concerning the grafting silver-fir - Abiesconcolor [(Gord. & Glend. Lindl] scions on white-fir (Abies alba Mill.rootstocks are reporting in this article. The double-side-veneer grafting method and the plastic tape and the ecological Ceraltin® wax were applied in four experimental variants. The side-veneer-grafting method and the classic materials, such as raffia and the hot wax were used at the two controls involved in this experiment. The grafting success expressed in percents, were transformed in arcsin square root of percent values, and a two-way analysis of variance was performed. Highly significant (p <0.001 statistical differences were found between grafting variants, including controls. The Duncan Multiple Range Test showed that the four experimental grafting variants were highly significantly (p < 0.01 better than the two controls. The grafting success of the best experimental variant has surpassed the two controls by 129 and 153%, respectively.Consequently, the double-side-veneer grafting method, the new developed plastic tape and the ecological Ceraltin® wax have contributed to this grafting success owing to which they are recommended to be used for grafting silver-fir ornamental trees.

  16. Effects of Temperature on Photosynthetic Rates in Korean Fir (Abies koreana) between Healthy and Dieback Population

    Su Young Woo; Jong-Hwan Lim; Don Koo Lee


    The present study was carried out on natural Korean fir forests (Abies koreana) growing In Mount Halla in Jeju Island, Korea (33°13-36′ N and 126° 12-57′ E). Mount Halla is the highest mountain (1950 m a.s.l.) in South Korea. On the Korean fir forests near the top of Mount Halla in Korea, we established permanent plots between dieback and healthy population. Each permanent plot includes both dieback and relatively healthy Korean fir individuals. Three sites in this study showed similar altitude, topographic position, aspects, slope, diameter at breast height, average height and ages. Net photosynthetic rates (PN) on different temperature regimes were evaluated to explain the forest dieback phenomenon on Korean fir populations. Light response curves were determined on three different temperature regimes: 15℃, 20℃and 25℃. The Irradiance response curve showed higher values in lower air temperatures. Generally, Irradiance response curves of healthy Korean fir populations were higher than the dieback population at all sites.

  17. Regulation of Water Use in the Southernmost European Fir (Abies pinsapo Boiss.: Drought Avoidance Matters

    Raúl Sánchez-Salguero


    Full Text Available The current scenario of global warming has resulted in considerable uncertainty regarding the capacity of forest trees to adapt to increasing drought. Detailed ecophysiological knowledge would provide a basis to forecast expected species dynamics in response to climate change. Here, we compare the water balance (stomatal conductance, xylem water potential, needle osmotic adjustment of Abies pinsapo, a relict drought-sensitive Mediterranean fir, along an altitudinal gradient. We related these variables to soil water and nutrient availability, air temperature, atmospheric water potential, and vapour pressure deficit during two consecutive years. Our results indicate that A. pinsapo closed stomata rapidly over a very narrow range of soil water availability and atmospheric dryness. This isohydric response during water stress suggests that this relict conifer relied on the plant hormone abscisic acid to maintain closed stomata during sustained drought, instead of needle desiccation to passively drive stomatal closure, needle osmotic adjustment or a plastic response of the xylem to different levels of water availability. Both the soil and foliar nutrient contents suggest that the studied populations are not limited by nutrient deficiencies, and drought was stronger in the warmer low-elevation areas.

  18. Review of Lepidoptera with trophic relationships to Picea abies (L. in the conditions of Czechia

    Modlinger Roman


    Full Text Available Trophic relationships of Lepidoptera (Insecta occurring in the territory of Czechia to the Norway spruce (Picea abies L. was evaluated on the basis of the excerption and critical evaluation of literature. Each species was classified into the following categories – spruce as the host plant, regular development on spruce, narrow trophic relationship, indirect relationship and episodical occurrence. The particular taxa were also characterized according to their distribution and the form of larval life was specified. The development on spruce was documented in 96 species of Lepidoptera, which represented less than 3% of taxa belonging to this group and being reported from Czechia. Of that, spruce was a common host plant for 67 species, 23 species were polyphagous and might develop on spruce, and 6 species belonged to soil species damaging spruce roots, mainly in forest nurseries. Among the species of Lepidoptera, which regularly develop on spruce in the Czech conditions, 55 species were classified. As narrow specialists with special trophic relationship to spruce, 33 taxa could be considered. There were 15 spruce species with forestry importance, which were able to outbreak their populations regularly or irregularly. Among spruce species it was possible to classify 16 taxa as rare. The provided information on Lepidoptera with trophic relationship to spruce is applicable also for other Central European areas. Besides the species with importance for forest pest management, also rare taxa, which can become endangered by climate change or by forest management, were indicated.

  19. Pre-growth mortality of Abies cilicica trees and mortality models performance.

    Carus, Serdar


    In this study, we compared tree-growth rates (basal area increment) from recently dead and living Taurus fir (Abies cilicica Carr.) trees in the Kovada lake Forest of Isparta, Turkey. For each dead tree, tree-growth rates were analyzed for the presence of pre-death growth depressions in the study area (number of sample plots = 11) in 2006. However, we compared both the magnitude and rate of growth prior to death to a control (living) group of trees. Basal area increment (BAI) averaged substantially less during the last 10 years before death than for control trees. Trees that died started diverging in growth, on average, 50-60 years before death. About 18% of trees that died had chronically slow growth, 46% had pronounced declines in growth, whereas 36% had good growth up to death. However, tree-ring-based growth patterns of dead and living Taurus fir trees were compared and used 12 mortality models that were derived using logistic regression from growth patterns of tree-ring series as predictor variables. The four models with the highest overall performance correctly classified 43.8-56.3% of all dead trees and 75.0-87.5% of all living trees, and they predicted 25.0-43.8% of all dead trees to die within 0-15 years prior to the actual year of death.

  20. Diverse ecological roles within fungal communities in decomposing logs of Picea abies.

    Ottosson, Elisabet; Kubartová, Ariana; Edman, Mattias; Jönsson, Mari; Lindhe, Anders; Stenlid, Jan; Dahlberg, Anders


    Fungal communities in Norway spruce (Picea abies) logs in two forests in Sweden were investigated by 454-sequence analyses and by examining the ecological roles of the detected taxa. We also investigated the relationship between fruit bodies and mycelia in wood and whether community assembly was affected by how the dead wood was formed. Fungal communities were highly variable in terms of phylogenetic composition and ecological roles: 1910 fungal operational taxonomic units (OTUs) were detected; 21% were identified to species level. In total, 58% of the OTUs were ascomycetes and 31% basidiomycetes. Of the 231 337 reads, 38% were ascomycetes and 60% basidiomycetes. Ecological roles were assigned to 35% of the OTUs, accounting for 62% of the reads. Wood-decaying fungi were the most common group; however, other saprotrophic, mycorrhizal, lichenized, parasitic and endophytic fungi were also common. Fungal communities in logs formed by stem breakage were different to those in logs originating from butt breakage or uprooting. DNA of specific species was detected in logs many years after the last recorded fungal fruiting. Combining taxonomic identification with knowledge of ecological roles may provide valuable insights into properties of fungal communities; however, precise ecological information about many fungal species is still lacking.

  1. Norway spruce (Picea abies) laccases: characterization of a laccase in a lignin-forming tissue culture.

    Koutaniemi, Sanna; Malmberg, Heli A; Simola, Liisa K; Teeri, Teemu H; Kärkönen, Anna


    Secondarily thickened cell walls of water-conducting vessels and tracheids and support-giving sclerenchyma cells contain lignin that makes the cell walls water impermeable and strong. To what extent laccases and peroxidases contribute to lignin biosynthesis in muro is under active evaluation. We performed an in silico study of Norway spruce (Picea abies (L.) Karst.) laccases utilizing available genomic data. As many as 292 laccase encoding sequences (genes, gene fragments, and pseudogenes) were detected in the spruce genome. Out of the 112 genes annotated as laccases, 79 are expressed at some level. We isolated five full-length laccase cDNAs from developing xylem and an extracellular lignin-forming cell culture of spruce. In addition, we purified and biochemically characterized one culture medium laccase from the lignin-forming cell culture. This laccase has an acidic pH optimum (pH 3.8-4.2) for coniferyl alcohol oxidation. It has a high affinity to coniferyl alcohol with an apparent Km value of 3.5 μM; however, the laccase has a lower catalytic efficiency (V(max)/K(m)) for coniferyl alcohol oxidation compared with some purified culture medium peroxidases. The properties are discussed in the context of the information already known about laccases/coniferyl alcohol oxidases of coniferous plants.

  2. Analysis of volatile components, fatty acids, and phytosterols of Abies koreana growing in Poland.

    Wajs-Bonikowska, Anna; Olejnika, Karol; Bonikowski, Radosław; Banaszczakb, Piotr


    Extracts and essential oils from seeds as well as essential oils from cone scales and needles with twigs of the Abies koreana population were studied. An analysis of Korean fir essential oils allowed us to determine 147 volatile compounds. The identified compounds constituted 97-99% of the seed, cone and needle oils. The main volatile in the seed and needle oils was limonene (56.6% and 23.4%, respectively), while the predominant volatile in cone oils was alpha-pinene (51.2%). Korean fir seeds provided a rich source of both essential oil (3.8-8.5%) and extract, which was isolated with a 24.5% yield and contained numerous groups of fatty acids and phytosterols (414 microg/100g extract). The most prominent fatty acids were unsaturated, among which linoleic (41.2%) and oleic (31.2%) fatty acid were the main ones while the dominant sterols were isomers of ergostadienol and beta-sitosterol. A. koreana seeds, cones and needles are a source of many volatile bioactive compounds while the seed extract, with a pleasant scent, contained not only volatiles, but also fractions rich in fatty acids and phytosterols. These facts make A. koreana essential oils and especially the seed extract potential components of cosmetics.

  3. Experimental versus modelled water use in mature Norway spruce (Picea abies exposed to elevated CO2

    Sebastian eLeuzinger


    Full Text Available Rising levels of atmospheric CO2 have often been reported to reduce plant water use. Such behaviour is also predicted by standard equations relating photosynthesis, stomatal conductance, and atmospheric CO2 concentration, which form the core of global dynamic vegetation models (DGVMs. Here, we provide first results from a free air CO2 enrichment (FACE experiment with naturally growing, mature (35 m Picea abies (L. (Norway spruce and compare them to simulations by the DGVM LPJ-GUESS. We monitored sap flow, stem water deficit, stomatal conductance, leaf water potential and soil moisture in five 35-40 m tall CO2-treated (550 ppm trees over two seasons. Using LPJ-GUESS, we simulated this experiment using climate data from a nearby weather station. While the model predicted a stable reduction of transpiration of between 9 and 18 % (at concentrations of 550-700ppm atmospheric CO2, the combined evidence from various methods characterising water use in our experimental trees suggest no changes in response to future CO2 concentrations. The discrepancy between the modelled and the experimental results may be a scaling issue: while dynamic vegetation models correctly predict leaf-level responses, they may not sufficiently account for the processes involved at the canopy and ecosystem scale, which could mitigate the first-order stomatal response.

  4. Shoot growth of mature Fagus sylvatica and Picea abies in relation to ozone

    Braun, Sabine [Institute for Applied Plant Biology, Sandgrubenstrasse 25, CH-4124 Schoenenbuch (Switzerland)]. E-mail:; Schindler, Christian [Institute for Social and Preventive Medicine, University of Basel, Steinengraben 49, CH-4051 Basel (Switzerland)]. E-mail:; Rihm, Beat [Meteotest, Fabrikstrasse 14, CH-3012 Berne (Switzerland)]. E-mail:; Flueckiger, Walter [Institute for Applied Plant Biology, Sandgrubenstrasse 25, CH-4124 Schoenenbuch (Switzerland)


    Epidemiological analysis of sequential growth data may be a tool in assessing ozone sensitivity of mature trees. Annual shoot growth of mature Fagus sylvatica in 83 Swiss permanent forest observation plots and of Picea abies in 61 plots was evaluated for 11 and 8 consecutive years, respectively, using branches harvested every 4 years. The data were assessed as annual deviation from average growth and related to fructification, ozone, meteorological parameters, and modelled soil water content using a mixed linear model. In beech, a significant association between ozone and shoot growth was observed which corresponded to a 7.4% growth reduction between 0 and 10 ppm h AOT40 (accumulated ozone over threshold 40). This is in the same order of magnitude as the response observed in experiments with seedlings. No interaction was found between ozone and drought parameters. In Norway spruce, shoot growth was neither associated with ozone nor with drought. - Epidemiological assessment of shoot growth suggests an ozone sensitivity of mature beech which is similar to seedlings.

  5. Groundwater quality assessment using geoelectrical and geochemical approaches: case study of Abi area, southeastern Nigeria

    Ebong, Ebong D.; Akpan, Anthony E.; Emeka, Chimezie N.; Urang, Job G.


    The electrical resistivity technique which involved the Schlumberger depth sounding method and geochemical analyses of water samples collected from boreholes was used to investigate the suitability of groundwater aquifers in Abi for drinking and irrigation purposes. Fifty randomly located electrical resistivity data were collected, modeled, and interpreted after calibration with lithologic logs. Ten borehole water samples were collected and analysed to determine anion, cation concentrations and some physical and chemical parameters, such as water colour, temperature, total dissolved solids, and electrical conductivity. The results show that the lithostratigraphy of the study area is composed of sands, sandstones (fractured, consolidated and loosed), siltstones, shales (compacted and fractured) of the Asu River Group, Eze-Aku Formation which comprises the aquifer units, and the Nkporo Shale Formation. The aquifer conduits are known to be rich in silicate minerals, and the groundwater samples in some locations show a significant amount of Ca2+, Mg2+, and Na+. These cations balanced the consumption of H+ during the hydrolytic alteration of silicate minerals. The geochemical analysis of groundwater samples revealed dominant calcium-magnesium-carbonate-bicarbonate water facies. Irrigation water quality parameters, such as sodium absorption ratio, percentage of sodium, and permeability index, were calculated based on the physico-chemical analyses. The groundwater quality was observed to be influenced by the interaction of some geologic processes but was classified to be good to excellent, indicating its suitability for domestic and irrigation purposes.

  6. Vertical foliage distribution determines the radial pattern of sap flux density in Picea abies.

    Fiora, Alessandro; Cescatti, Alessandro


    Understanding the causes determining the radial pattern of sap flux density is important both for improving knowledge of sapwood functioning and for up-scaling sap flow measurements to canopy transpiration and ecosystem water use. To investigate the anatomical connection between whorls and annual sapwood rings, pruning-induced variation in the radial pattern of sap flux density was monitored with Granier probes in a 35-year-old Picea abies (L.) Karst tree that was pruned from the crown bottom up. Modifications in the radial pattern of sap flux density were quantified by a shape index (SI), which varies with the relative contribution of the outer and inner sapwood to tree transpiration. The SI progressively diminished during bottom up pruning, indicating a significant reduction in sap flow contribution of the inner sapwood. Results suggest that the radial pattern of sap flux density depends mainly on the vertical distribution of foliage in the crown, with lower shaded branches hydraulically connected with inner sapwood and upper branches connected with the outer rings.

  7. Wood anatomical relationships within Abies spp. from the Mediterranean area: a phyletic approach

    Esteban, L. G.; Palacios, P. de; Garcia Fernandez, F.; Martin, J. A.


    An analysis was made of the wood anatomy of seven species, one subspecies and two varieties of the genus Abies from the Mediterranean area (A. alba, A. borisii-regis, A. cephalonica, A. cilicica, A. nordmanniana, A. nordmanniana subsp. equi-trojani, A. numidica, A. pinsapo, A. pinsapo var. marocana and A. pinsapo var. tazaotana) in order to find phyletically relevant features to help clarify and complement the evolutionary patterns based on molecular studies. The wood structure within the genus was qualitatively similar, except for specific features characteristic of certain provenances. However, the wood biometry allowed the different taxa to be grouped in accordance with their anatomical similarity. A. alba, A. pinsapo, A. pinsapo var. marocana and A. pinsapo var. tazaotana possess biometric features which distinguish them from the other Mediterranean firs. Furthermore, A. numidica showed biometric features which distinguish it from the eastern firs (A. borisii-regis, A. cephalonica, A. cilicica, A. nordmanniana and A. nordmanniana subsp. equi-trojani) and place it closer to certain Iberian populations of A. pinsapo. The maximum ray height in number of cells, frequency of rays with more than 30 cells and tracheid length can be regarded as specific patterns of the Mediterranean fir phylum. (Author) 72 refs.

  8. General Toxicity and Antifungal Activity of a New Dental Gel with Essential Oil from Abies Sibirica L

    Noreikaitė, Aurelija; Ayupova, Rizvangul; Satbayeva, Elmira; Seitaliyeva, Aida; Amirkulova, Marzhan; Pichkhadze, Guram; Datkhayev, Ubaidilla; Stankeviandccaron;ius, Edgaras


    Background The aim of this study was to analyze the antifungal activity and the general toxicity of a new dental gel containing essential oil from the tree Abies sibirica L., which grows in the Republic of Kazakhstan. Material/Methods The essential oil from Abies sibirica L. was obtained by microwave heating method using the STARTE Microwave Extraction System. Adjutants used to prepare the oil were carbomer 974P, glycerin, polysorbate 80, xylitol, triethanolamine, and purified water, all allowed for medical usage. The antifungal activity of the essential oil was assessed by monitoring the optical density of Candida albicans in a microplate reader. The safety was determined by analyzing the acute and subacute toxicity. Results The essential oil obtained by the microwave heating method revealed a higher antifungal activity in comparison with the essential oil obtained by the steam distillation method. No obvious changes were detected in guinea pigs following cutaneous application of the gel. Enteral administration of the essential oil caused minimal functional and histological changes in mice after 4 weeks. The new harmless dental gel containing pine oil from Abies sibirica L. was provided for the purposes of this particular clinical research. Conclusions The high antifungal activity of the gel is the basis for more in-depth studies on its safety and pharmacological activity. PMID:28132065

  9. Mycorrhiza of the host-specific Lactarius deterrimus on the roots of Picea abies and Arctostaphylos uva-ursi.

    Mühlmann, O; Göbl, F


    The ectomycorrhizal basidiomycete species Lactarius deterrimus Gröger is considered to be a strictly host-specific mycobiont of Picea abies (L.) Karst. However, we identified arbutoid mycorrhiza formed by this fungus on the roots of Arctostaphylos uva-ursi (L.) Spreng. in a mixed stand at the alpine timberline; typical ectomycorrhiza of P. abies were found in close relation. A. uva-ursi is known as an extremely unspecific phytobiont. The mycorrhizae of both associations are described and compared morphologically. The mycorrhiza formed by L. deterrimus on both A. uva-ursi and P. abies show typical ectomycorrhizal features such as a hyphal mantle and a Hartig net. The main difference between the mycorrhizal symbioses with the different phytobionts is the occurrence of intracellular hyphae in the epidermal cells of A. uva-ursi. This emphasizes the importance of the plant partner for mycorrhizal anatomy. This is the first report of a previously considered host-specific ectomycorrhizal fungus in association with A. uva-ursi under natural conditions. The advantages of this loose specificity between the fungus and plant species is discussed.

  10. General Toxicity and Antifungal Activity of a New Dental Gel with Essential Oil from Abies Sibirica L.

    Noreikaitė, Aurelija; Ayupova, Rizvangul; Satbayeva, Elmira; Seitaliyeva, Aida; Amirkulova, Marzhan; Pichkhadze, Guram; Datkhayev, Ubaidilla; Stankevičius, Edgaras


    BACKGROUND The aim of this study was to analyze the antifungal activity and the general toxicity of a new dental gel containing essential oil from the tree Abies sibirica L., which grows in the Republic of Kazakhstan. MATERIAL AND METHODS The essential oil from Abies sibirica L. was obtained by microwave heating method using the STARTE Microwave Extraction System. Adjutants used to prepare the oil were carbomer 974P, glycerin, polysorbate 80, xylitol, triethanolamine, and purified water, all allowed for medical usage. The antifungal activity of the essential oil was assessed by monitoring the optical density of Candida albicans in a microplate reader. The safety was determined by analyzing the acute and subacute toxicity. RESULTS The essential oil obtained by the microwave heating method revealed a higher antifungal activity in comparison with the essential oil obtained by the steam distillation method. No obvious changes were detected in guinea pigs following cutaneous application of the gel. Enteral administration of the essential oil caused minimal functional and histological changes in mice after 4 weeks. The new harmless dental gel containing pine oil from Abies sibirica L. was provided for the purposes of this particular clinical research. CONCLUSIONS The high antifungal activity of the gel is the basis for more in-depth studies on its safety and pharmacological activity.

  11. Stand-structural effects on Heterobasidion abietinum-related mortality following drought events in Abies pinsapo.

    Linares, Juan Carlos; Camarero, Jesús Julio; Bowker, Matthew A; Ochoa, Victoria; Carreira, José Antonio


    Climate change may affect tree-pathogen interactions. This possibility has important implications for drought-prone forests, where stand dynamics and disease pathogenicity are especially sensitive to climatic stress. In addition, stand structural attributes including density-dependent tree-to-tree competition may modulate the stands' resistance to drought events and pathogen outbreaks. To assess the effects of stand structure on root-rot-related mortality after severe droughts, we focused on Heterobasidion abietinum mortality in relict Spanish stands of Abies pinsapo, a drought-sensitive fir. We compared stand attributes and tree spatial patterns in three plots with H. abietinum root-rot disease and three plots without root-rot. Point-pattern analyses were used to investigate the scale and extent of mortality patterns and to test hypotheses related to the spread of the disease. Dendrochronology was used to date the year of death and to assess the association between droughts and growth decline. We applied a structural equation modelling approach to test if tree mortality occurs more rapidly than predicted by a simple distance model when trees are subjected to high tree-to-tree competition and following drought events. Contrary to expectations of drought mortality, the effect of precipitation on the year of death was strong and negative, indicating that a period of high precipitation induced an earlier tree death. Competition intensity, related to the size and density of neighbour trees, also induced an earlier tree death. The effect of distance to the disease focus was negligible except in combination with intensive competition. Our results indicate that infected trees have decreased ability to withstand drought stress, and demonstrate that tree-to-tree competition and fungal infection act as predisposing factors of forest decline and mortality.

  12. Evaluation of growth disturbances of Picea abies (L.) Karst. to disturbances caused by landslide movements

    Šilhán, Karel


    Dendrogeomorphic methods are frequently used in landslide analyses. Although methods of landslide dating based on tree rings are well developed, they still indicated many questions. The aim of this study was to evaluate the frequently used theoretical scheme based on the event-response relationship. Seventy-four individuals of Norway spruce (Picea abies (L.) Karst.) exhibiting visible external disturbance, were sampled on the Girová landslide (the largest historical flow-like landslide in the Czech Republic). This landslide reactivated in May 2010, and post-landslide tree growth responses were studied in detail. These growth responses were compared with the intensity and occurrence of visible external tree disturbance: tilted stems, damaged root systems, and decapitation. Twenty-nine trees (39.2%) died within one to four years following the 2010 landslide movement. The trees that died following the landslide movement were significantly younger and displayed significantly greater stem tilting than the live trees. Abrupt growth suppression was a more-frequent response among the dead trees, whereas growth release dominated among the live trees. Only two trees (2.7%) created no reaction wood in response to the landslide movement. Forty-four percent of the trees started to produce reaction wood structure after a delay, which generally spanned one year. Some eccentric growth was evident in the tree rings of the landslide year and was significant in the first years following the landslide movement. Missing rings were observed only on the upper sides of the stems, and no false tree rings were observed. The results confirm the general validity of event-response relationship, nevertheless this study points out the limitations and uncertainties of this generally accepted working scheme.

  13. Insights into Diterpene Cyclization from Structure of Bifunctional Abietadiene Synthase from Abies grandis

    Zhou, Ke; Gao, Yang; Hoy, Julie A.; Mann, Francis M.; Honzatko, Richard B.; Peters, Reuben J. (Iowa State)


    Abietadiene synthase from Abies grandis (AgAS) is a model system for diterpene synthase activity, catalyzing class I (ionization-initiated) and class II (protonation-initiated) cyclization reactions. Reported here is the crystal structure of AgAS at 2.3 {angstrom} resolution and molecular dynamics simulations of that structure with and without active site ligands. AgAS has three domains ({alpha}, {beta}, and {gamma}). The class I active site is within the C-terminal {alpha} domain, and the class II active site is between the N-terminal {gamma} and {beta} domains. The domain organization resembles that of monofunctional diterpene synthases and is consistent with proposed evolutionary origins of terpene synthases. Molecular dynamics simulations were carried out to determine the effect of substrate binding on enzymatic structure. Although such studies of the class I active site do lead to an enclosed substrate-Mg{sup 2+} complex similar to that observed in crystal structures of related plant enzymes, it does not enforce a single substrate conformation consistent with the known product stereochemistry. Simulations of the class II active site were more informative, with observation of a well ordered external loop migration. This 'loop-in' conformation not only limits solvent access but also greatly increases the number of conformational states accessible to the substrate while destabilizing the nonproductive substrate conformation present in the 'loop-out' conformation. Moreover, these conformational changes at the class II active site drive the substrate toward the proposed transition state. Docked substrate complexes were further assessed with regard to the effects of site-directed mutations on class I and II activities.

  14. Isoprene and terpenoid emissions from Abies alba: Identification and emission rates under ambient conditions

    Pokorska, Olga; Dewulf, Jo; Amelynck, Crist; Schoon, Niels; Šimpraga, Maja; Steppe, Kathy; Van Langenhove, Herman


    In this study, biogenic volatile organic compound (BVOC) emissions from Abies alba were studied under ambient conditions in Flanders (Belgium). Emission patterns and rates were investigated from April till November 2010 by using the dynamic branch enclosure technique. The present work revealed that A. alba is an isoprene emitter, with isoprene accounting for 86-93% of total BVOC emissions, except during budburst (67%) in May. The emission spectrum of A. alba consisted of 27 compounds. Next to isoprene, the main emitted compounds were α-pinene, β-pinene, camphene and limonene. BVOC emissions showed a peak in June after development of the young needles, followed by a constant emission during summer months and September and a decrease in October. In all the samples isoprene was the most abundant compound with standardized emission rates between 27 μg g(dw)-1 h-1 in June and 4.6 μg g(dw)-1 h-1 in October, while the total standardized terpenoid emission rates ranged from 2.85 μg g(dw)-1 h-1 in June to 0.26 μg g(dw)-1 h-1 in October. The obtained average β coefficients according to the temperature dependent algorithm of Guenther et al. (1993) during April-June, July, August and September-October were as follows: for terpenoids 0.12 ± 0.03, 0.11 ± 0.05, 0.12 ± 0.04, 0.24 ± 0.01 K-1 and sesquiterpenes (SQTs) 0.09 ± 0.02, 0.11 ± 0.01, 0.10 ± 0.05, 0 K-1, respectively. Overall, isoprene detected in this study was never quantified in previous studies on A. alba and this finding could have a significant impact on the regional BVOCs budget. Therefore, the result of this study is very important for modeling and local air quality.

  15. Heterobasidion annosum root rot in Picea abies: Variability in aggressiveness and resistance

    Swedjemark, G.


    In greenhouse experiments 3-4 year-old plants of Picea abies and Pinus sylvestris were inoculated with the root rot fungus Heterobasidion annosum. The growth rates of 25 S and 14 P isolates of the fungus were measured in the sapwood of about 25 rametes each of 98 Norway spruce clones and in 150 seedlings each of Norway spruce and Scots pine. Correlations were determined between fungal sapwood growth and a variety of variables, i.e. the physical stage, growth rhythm, size and condition of the cuttings, provenance and the growth capacity of the clones estimated in field tests. Genetic parameters such as phenotypic and genotypic variance, genotypic coefficient of variance and broad sense heritability were calculated. H. annosum infection incidence was close to 100 % for inoculated Norway spruce clones, and the mortality rate was about 1 %. Fungal growth in sapwood differed significantly among clones and among fungal isolates. The genotypic correlation coefficient was large (30 %) and broad sense heritability was 0.35. This suggests that good selection progress can be achieved in breeding programs. The physical stage (bud-flush, vegetative and post bud-set stage) of the clones contributed significantly to the total variation in fungal growth. The population structure of H. annosum was studied in two 25-year-old, first generation stands of Norway spruce. The stands had been thinned one and seven years earlier, respectively. All stumps and remaining trees were sampled for H. annosum isolates. The isolates were tested with somatic incompatibility to detect single genets and the distribution of genets in the stand was determined. About 70 % of the 7-year-old thinning stumps were colonized by H, annosum, and 50 % of these stumps were colonized by more than one fungal genet. Sixty-three percent of the remaining trees were infected with H. annosum but among them there was only one genet per tree. 104 refs, 12 figs

  16. Climate signal in a Picea abies tree ring chronology in Eastern Romanian Carpathians

    Vieru, I.; Holobâcǎ, I. H.; Pop, O.; Irimuş, I. A.; Georgescu, M.


    Trees are both sensitive and adaptive to environmental change; consequently they are often used as indicators for past climate variability. The correlation between climate and growth of spruce (Picea abies (L.) Karst) in Eastern Romanian Carpathians was tested by choosing a sample site situated beneath the upper timberline (where the climatic factor would be more restrictive), at the altitude of 900 - 1000 m, on the north facing left slope of the Gošman valley (Neamt county). The tree ring width chronology covering most of the last three centuries was derived from the 25 living spruce trees sampled. The climatic dataset comprises monthly average temperature and precipitation values from the closest 7 weather stations, as well as for the nearest grid point of CRU TS. 3.1 dataset. One of the methods consists of detrending and standardization the tree ring using a negative exponential function and a linear function, the average population index being calculated using a bi-weight robust mean. The statistical significance of correlation is tested with the bootstrap method and the coefficients that exceed the 95% confidence level are highlighted. Preliminary results indicate a significant correlation between the tree ring chronology and the average winter temperatures (November, December and January) of the grid dataset. The length of this temperature dataset also allows the performance of correlation with evolutionary and moving intervals, which is not possible in the case of instrumental data. Further on, differences in standardization methods used and the corresponding results are detailed. The results of Regional Curve Standardization are of particular interest, considering the limited length of the chronology, and the possible bias introduced by the modern sample of uneven aged trees that is prone to a contemporaneous-growth-rate-bias. Keywords: dendroclimatology, tree ring, standardization, correlation, RCS method.

  17. Effects of tree architecture on pollen dispersal and mating patterns in Abies pinsapo Boiss. (Pinaceae).

    Sánchez-Robles, Jose M; García-Castaño, Juan L; Balao, Francisco; Terrab, Anass; Navarro-Sampedro, Laura; Tremetsberger, Karin; Talavera, Salvador


    Plant architecture is crucial to pollination and mating in wind-pollinated species. We investigated the effect of crown architecture on pollen dispersal, mating system and offspring quality, combining phenotypic and genotypic analyses in a low-density population of the endangered species Abies pinsapo. A total of 598 embryos from three relative crown height levels (bottom, middle and top) in five mother plants were genotyped using eleven nuclear microsatellite markers (nSSRs). Paternity analysis and mating system models were used to infer mating and pollen dispersal parameters. In addition, seeds were weighed (N = 16 110) and germinated (N = 736), and seedling vigour was measured to assess inbreeding depression. Overall, A. pinsapo shows a fat-tailed dispersal kernel, with an average pollen dispersal distance of 113-227 m, an immigration rate of 0.84-26.92%, and a number of effective pollen donors (Nep ) ranging between 3.5 and 11.9. We found an effect of tree height and relative crown height levels on mating parameters. A higher proportion of seeds with embryo (about 50%) and a higher rate of self-fertilization (about 60%) were found at the bottom level in comparison with the top level. Seed weight and seedling vigour are positively related. Nevertheless, no differences were found in seed weight or in seedling-related variables such as weight and length of aerial and subterranean parts among the different relative crown height levels, suggesting that seeds from the more strongly inbred bottom level are not affected by inbreeding depression. Our results point to vertical isotropy for outcross-pollen and they suggest that self-pollen may ensure fertilization when outcross-pollen is not available in low-density population.

  18. Insights into Diterpene Cyclization from Structure of Bifunctional Abietadiene Synthase from Abies grandis*

    Zhou, Ke; Gao, Yang; Hoy, Julie A.; Mann, Francis M.; Honzatko, Richard B.; Peters, Reuben J.


    Abietadiene synthase from Abies grandis (AgAS) is a model system for diterpene synthase activity, catalyzing class I (ionization-initiated) and class II (protonation-initiated) cyclization reactions. Reported here is the crystal structure of AgAS at 2.3 Å resolution and molecular dynamics simulations of that structure with and without active site ligands. AgAS has three domains (α, β, and γ). The class I active site is within the C-terminal α domain, and the class II active site is between the N-terminal γ and β domains. The domain organization resembles that of monofunctional diterpene synthases and is consistent with proposed evolutionary origins of terpene synthases. Molecular dynamics simulations were carried out to determine the effect of substrate binding on enzymatic structure. Although such studies of the class I active site do lead to an enclosed substrate-Mg2+ complex similar to that observed in crystal structures of related plant enzymes, it does not enforce a single substrate conformation consistent with the known product stereochemistry. Simulations of the class II active site were more informative, with observation of a well ordered external loop migration. This “loop-in” conformation not only limits solvent access but also greatly increases the number of conformational states accessible to the substrate while destabilizing the nonproductive substrate conformation present in the “loop-out” conformation. Moreover, these conformational changes at the class II active site drive the substrate toward the proposed transition state. Docked substrate complexes were further assessed with regard to the effects of site-directed mutations on class I and II activities. PMID:22219188

  19. Number of chromosomes and chromosome rearrangement of Norway spruce Picea abies (L. H. Karst. in the forests of Rilo-Rhodope mountain in Bulgaria

    A. N. Tashev


    Full Text Available Comparative investigations of chromosome numbers and chromosome rearrangements in populations of Norway spruce (Picea abies (L. H. Karst. growing in the territory of State Forest Service «Garmen», «Eleshnitsa», «Yakoruda», «Dobrinishte» in Rila-Rhodope mountain region (Bulgaria. Populations are located at the southern border of species range, and protected according to Bern Convention and EC Habitat Directive 92/43. It was found that diploid set of seed progeny of P. abies from the 4 populations studied includes 24 chromosomes (2n = 2x = 24. Mixoploidy (2n = 24, 36; 2n = 24, 48; 2n = 24, 36, 48 was detected in some germinating seeds of all studied populations of P. abies. Metaphase cells of germinating seeds of P. abies from State Forest Service «Garmen» and «Eleshnitsa» contain supernumerary, or B-chromosomes, while the variability of their number and occurrence was observed. In cells of germinating seeds of P. abies from State Forest Service «Yakoruda», «Dobrinishte», B-chromosomes are not revealed. Some chromosome rearrangements such as fragments and ring chromosomes were revealed in metaphase cells of P. abies from populations of «Garmen», «Eleshnitsa» and «Dobrinishte». Probably occurrence of mixoploids, B chromosomes and chromosome rearrangements in P. abies populations growing in Rila-Rhodope mountain region is connected with adaptation of trees to the extreme environmental conditions and such a serious factor that presently forests in the studied region located in the zone of natural radioactivity and in the past, the region of study was subjected to substantial anthropogenic pressure due to uranium extraction industry.

  20. Streamwater chemistry and flow dynamics along vegetation-soil gradient in a subalpine Abies fabri forest watershed,China

    SHAN Bao-qing; WANG Wei-dong; YIN Cheng-qing


    Streamwater chemistry and spatial flow dynamics from a subalpine Abies fabri forest in an experimental watershed located in the east slope of Gongga Mountain were analyzed to gain insights into the gradient effect of primary community succession on the stream biogeochemical process. Results showed that high sand content(exceeding 80%) and porosity in the soil(exceeding 20% in A horizon and 35% in B horizon), as well as a thick humus layer on the soil surface, made the water exchange quickly in the Huangbengliu(HBL) watershed. Consequently, no surface runoff was observed, and the stream discharge changed rapidly with the daily precipitation. The flow trends of base ions in the stream water were influenced by the Abies fabri succession gradient. Ca2+, HCO3- and SO42- were the dominant anions in the streamwater in this region. A significant difference of Ca2+, HCO3- and SO42- concentration exported between the succession stages in the watershed can be found. But they had the similar temporal change in the stream flow. Ca2+, HCO3- and SO42- showed significantly negative correlations with the daily precipitation and the stream discharge. Concentrations of Cl-, Na+, K+, and Mg2+ were low in all streamwaters monitored and we observed no differences along the Abies fabri succession gradient. Low ratios of Na:(Na+Ca) (range from 0.1 to 0.2) implied cations were from bedrock weathering(internal source process in the soil system) in this region. But, a variance analysis showed there were almost no differences between rainwater and streamwaters for Mg2+, Na+, K+, and Cl- concentrations. This indicated that they might be come from rainfall inputs (external source). We suggest that the highly mobile capacity, rapid water exchange between precipitation and discharge, and long-term export lead to this observed pattern.

  1. Holocene variability in the range distribution and abundance of Pinus, Picea abies, and Quercus in Romania; implications for their current status

    Feurdean, Angelica; Tanţău, Ioan; Fărcaş, Sorina


    This paper examines fourteen fossil pollen datasets from Romania. It aims to investigate the temporal and spatial variability in the range distribution and abundance of three forest taxa, Pinus, Picea abies, and Quercus, during the Holocene. This is essential for understanding their current status in the forests of Eastern Europe, the conditions under which they arose, and the timing and processes responsible for their variability. Results from this synthesis do not indicate any apparent time lag in the establishment of Pinus diploxylon type ( Pinus sylvestris and Pinus mugo), Pinus cembra, P. abies, and Quercus across Romania within the limits of the dating resolution. However, the onset of the mass expansion of P. abies was not uniform, spreading earlier from sites in the western and north-western Carpathians (11,000-10,500 yr BP) than in the east (10,000 yr BP). We found that sites from the western, north-western, and northern Carpathians contained higher abundances of P. abies, whilst Quercus was in higher abundances in sites from the east, but there was no regional distinctiveness in the abundance of Pinus across the study area. However, P. diploxylon type was found in much higher abundance than P. cembra. Additionally, results indicate a greater proportion of Pinus (mainly P. diplxylon type) at high elevations, P. abies at mid to high elevations, and Quercus at low elevations (Pinus in the early Holocene boreal forest is likely the legacy of its local glacial refugia, fast life history strategies, high stress tolerance, and large habitat availability. In contrast, Pinus exhibited poor competitive abilities and was quickly replaced with P. abies and temperate deciduous taxa after 10,500 yr BP. P. abies has persisted in large abundances at higher elevations (above 1000 m) until the present day, as a result of good competitive abilities, and resilience to climate change and disturbance. The long-term dominance of P. abies appears to have been spatially

  2. ABI Research:2016年家庭自动化系统出货量将突破1200万


    ABI Research最新报告指出,家庭自动化系统(home automation system)2011年度全球出货量将达到180万套,并预计2016年将达1200万套。基于不需添加新电缆线的无线技术如ZigBee等标准的解决方案将系统的成本降低,成为该市场强劲成长的动力。

  3. Are Early Somatic Embryos of the Norway Spruce (Picea abies (L. Karst. Organised?

    Jiri Petrek

    Full Text Available Somatic embryogenesis in conifer species has great potential for the forestry industry. Hence, a number of methods have been developed for their efficient and rapid propagation through somatic embryogenesis. Although information is available regarding the previous process-mediated generation of embryogenic cells to form somatic embryos, there is a dearth of information in the literature on the detailed structure of these clusters.The main aim of this study was to provide a more detailed structure of the embryogenic tissue clusters obtained through the in vitro propagation of the Norway spruce (Picea abies (L. Karst.. We primarily focused on the growth of early somatic embryos (ESEs. The data on ESE growth suggested that there may be clear distinctions between their inner and outer regions. Therefore, we selected ESEs collected on the 56th day after sub-cultivation to dissect the homogeneity of the ESE clusters. Two colourimetric assays (acetocarmine and fluorescein diacetate/propidium iodide staining and one metabolic assay based on the use of 2,3,5-triphenyltetrazolium chloride uncovered large differences in the metabolic activity inside the cluster. Next, we performed nuclear magnetic resonance measurements. The ESE cluster seemed to be compactly aggregated during the first four weeks of cultivation; thereafter, the difference between the 1H nuclei concentration in the inner and outer clusters was more evident. There were clear differences in the visual appearance of embryos from the outer and inner regions. Finally, a cluster was divided into six parts (three each from the inner and the outer regions of the embryo to determine their growth and viability. The innermost embryos (centripetally towards the cluster centre could grow after sub-cultivation but exhibited the slowest rate and required the longest time to reach the common growth rate. To confirm our hypothesis on the organisation of the ESE cluster, we investigated the effect of

  4. ABI-like transcription factor gene TaABL1 from wheat improves multiple abiotic stress tolerances in transgenic plants.

    Xu, Dong-Bei; Gao, Shi-Qing; Ma, You-Zhi; Xu, Zhao-Shi; Zhao, Chang-Ping; Tang, Yi-Miao; Li, Xue-Yin; Li, Lian-Cheng; Chen, Yao-Feng; Chen, Ming


    The phytohormone abscisic acid (ABA) plays crucial roles in adaptive responses of plants to abiotic stresses. ABA-responsive element binding proteins (AREBs) are basic leucine zipper transcription factors that regulate the expression of downstream genes containing ABA-responsive elements (ABREs) in promoter regions. A novel ABI-like (ABA-insensitive) transcription factor gene, named TaABL1, containing a conserved basic leucine zipper (bZIP) domain was cloned from wheat. Southern blotting showed that three copies were present in the wheat genome. Phylogenetic analyses indicated that TaABL1 belonged to the AREB subfamily of the bZIP transcription factor family and was most closely related to ZmABI5 in maize and OsAREB2 in rice. Expression of TaABL1 was highly induced in wheat roots, stems, and leaves by ABA, drought, high salt, and low temperature stresses. TaABL1 was localized inside the nuclei of transformed wheat mesophyll protoplast. Overexpression of TaABL1 enhanced responses of transgenic plants to ABA and hastened stomatal closure under stress, thereby improving tolerance to multiple abiotic stresses. Furthermore, overexpression of TaABL1 upregulated or downregulated the expression of some stress-related genes controlling stomatal closure in transgenic plants under ABA and drought stress conditions, suggesting that TaABL1 might be a valuable genetic resource for transgenic molecular breeding.

  5. Impact Of Pine (Pinus sylvestris L. And Spruce (Picea abies (L. Karst. Bark Extracts On Important Strawberry Pathogens

    Minova Sandra


    Full Text Available Phytopathogenic fungi induced considerable economic losses in strawberry production industry; therefore, more attention should be paid to development and implementation of preventative treatment that is environmentally friendly. Coniferous trees produce a wide variety of compounds, such as terpenoids and phenolics. Several studies are known on fungicidal activity of different components of coniferous tree bark. The aim of this study was to evaluate in vitro pine (Pinus sylvestris L. and spruce (Picea abies (L. Karst. bark ethanol extracts impact on pathogenous fungi causing diseases of strawberries. Products of processed pine (Pinus sylvestris and spruce (Picea abies bark were tested. During 2011 to 2013, several in vitro experiments were carried out to test the effectiveness of pine and spruce bark extracts against various phytopathogenic fungi isolated from strawberries: Botrytis cinerea, Colletotrichum acutatum, Phytophthora cactorum and Mycosphaerella fragariae. Radial growth tests showed that coniferous bark extracts inhibit mycelial growth of B. cinerea, C. acutatum, P. cactorum and M. fragariae. Extracts had the highest antifungal effect on B. cinerea two and five days after inoculation (p < 0.05. Bark extracts can reduce the sporulation of B. cinerea, C. acutatum and P. cactorum.

  6. Network Performance Evaluation of Abis Interface over DVB-S2 in the GSM over Satellite Network

    S. B. Musabekov


    Full Text Available This paper deals with establishing a GSM link over Satellite. Abis interface, which is defined between Base Transceiver Station (BTS and Base Station Controller (BSC, in a GSM network is considered here to be routed over the Satellite. The satellite link enables a quick and cost-effective GSM link in meagerly populated areas. A different scenario comparison was done to understand the impact of Satellite environment on network availability comparing to terrestrial scenario. We have implemented an Abis interface over DVB S2 in NS2 and evaluated the performance over the high delay and loss satellite channel. Network performance was evaluated with respect to Satellite channel delay and DVB S2 encapsulation efficiency under different amount of user traffic and compared with the terrestrial scenario. The results clearly showed an increased amount of SDCCH and TCH channels required in the case of satellite scenario for the same amount of traffic in comparison to conventional terrestrial scenario. We have optimized the parameters based on the simulation results. Link budget estimation considering DVB-S2 platform was done to find satellite bandwidth and cost requirements for different network setups.

  7. A pyrosequencing insight into sprawling bacterial diversity and community dynamics in decaying deadwood logs of Fagus sylvatica and Picea abies.

    Hoppe, Björn; Krger, Krüger; Kahl, Tiemo; Arnstadt, Tobias; Buscot, François; Bauhus, Jürgen; Wubet, Tesfaye


    Deadwood is an important biodiversity hotspot in forest ecosystems. While saproxylic insects and wood-inhabiting fungi have been studied extensively, little is known about deadwood-inhabiting bacteria. The study we present is among the first to compare bacterial diversity and community structure of deadwood under field conditions. We therefore compared deadwood logs of two temperate forest tree species Fagus sylvatica and Picea abies using 16S rDNA pyrosequencing to identify changes in bacterial diversity and community structure at different stages of decay in forest plots under different management regimes. Alphaproteobacteria, Acidobacteria and Actinobacteria were the dominant taxonomic groups in both tree species. There were no differences in bacterial OTU richness between deadwood of Fagus sylvatica and Picea abies. Bacteria from the order Rhizobiales became more abundant during the intermediate and advanced stages of decay, accounting for up to 25% of the entire bacterial community in such logs. The most dominant OTU was taxonomically assigned to the genus Methylovirgula, which was recently described in a woodblock experiment of Fagus sylvatica. Besides tree species we were able to demonstrate that deadwood physico-chemical properties, in particular remaining mass, relative wood moisture, pH, and C/N ratio serve as drivers of community composition of deadwood-inhabiting bacteria.

  8. [Spatial patterns of dominant tree species in sub-alpine Betula-Abies forest in West Sichuan of China].

    Miao, Ning; Liu, Shi-Rong; Shi, Zuo-Min; Yu, Hong; Liu, Xing-Liang


    Based on the investigation in a 4 hm2 Betula-Abies forest plot in sub-alpine area in West Sichuan of China, and by using point pattern analysis method in terms of O-ring statistics, the spatial patterns of dominant species Betula albo-sinensis and Abies faxoniana in different age classes in study area were analyzed, and the intra- and inter-species associations between these age classes were studied. B. albo-sinensis had a unimodal distribution of its DBH frequency, indicating a declining population, while A. faxoniana had a reverse J-shaped pattern, showing an increasing population. All the big trees of B. albo-sinensis and A. faxoniana were spatially in random at all scales, while the medium age and small trees were spatially clumped at small scales and tended to be randomly or evenly distributed with increasing spatial scale. The maximum aggregation degree decreased with increasing age class. Spatial association mainly occurred at small scales. A. faxoniana generally showed positive intra-specific association, while B. albo-sinensis generally showed negative intra-specific association. For the two populations, big and small trees had no significant spatial association, but middle age trees had negative spatial association. Negative inter-specific associations of the two populations were commonly found in different age classes. The larger the difference of age class, the stronger the negative inter-specific association.

  9. A postal survey of data in general practice on the prevalence of Acquired Brain Injury (ABI) in patients aged 18-65 in one county in the west of Ireland.

    Finnerty, Fionnuala


    BACKGROUND: Very little is known about the prevalence of acquired brain injury (ABI) in Ireland. ABI prevalence has previously been obtained from Belgian general practitioners using a postal survey. We attempted to ascertain the prevalence of ABI in County Mayo through a postal survey of all general practitioners in the county.The specific objectives of this project were to:1. identify whether general practitioners are a. aware of patients with ABI aged 18-65 in their practices b. able to provide prevalence data on ABI in patients aged 18-65 c. able to provide data on age, gender and patient diagnosis 2. analyse prevalence of ABI from any available data from general practitioners. METHODS: A pilot postal survey was performed initially in order to assess the feasibility of the study. It was established that general practitioners did have the necessary information required to complete the questionnaire. A main postal survey was then undertaken. A postal questionnaire was administered to all general practices in County Mayo in the west of Ireland (n = 59). The response rate was 32.2% (n = 19). RESULTS: General practitioners who replied on behalf of their practice could provide data on patient age, gender and diagnosis. In the nineteen practices, there were 57 patients with ABI. The age-specific prevalence of ABI in the area surveyed was estimated at 183.7 per 100,000. The mean patient population per practice was 2,833 (SD = 950). There were found to be significantly more patients with ABI in rural areas than urban areas (p = 0.006). There were also significant differences in the ages of patients in the different ABI categories. Patients whose ABI was of traumatic origin were significantly younger than those patients with ABI of haemorrhagic origin (p = 0.002). CONCLUSION: Although this is a small-scale study, we have ascertained that general practitioners do have data on patients with ABI. Also, some prevalence data now exist where none was available before. These can

  10. Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment

    Jeon, Je-Seung; Kim, Ji Hoon; Park, Chae Lee; Kim, Chul Young


    Preparative separation of antioxidant constituents from the leaves of Abies koreana Wilson (Pinaceae) was performed by centrifugal partition chromatography (CPC) with a two-phase solvent system of ethyl acetate-isopropanol-water (9:1:10, v/v) target-guided by DPPH•-HPLC experiment. In DPPH•-HPLC exp

  11. Binding of ABI4 to a CACCG motif mediates the ABA-induced expression of the ZmSSI gene in maize (Zea mays L.) endosperm.

    Hu, Yu-Feng; Li, Yang-Ping; Zhang, Junjie; Liu, Hanmei; Tian, Mengliang; Huang, Yubi


    Starch synthase I (SSI) contributes the majority of the starch synthase activity in developing maize endosperm. In this work, the effects of various plant hormones and sugars on the expression of the starch synthase I gene (ZmSSI) in developing maize endosperms were examined. The accumulation of ZmSSI mRNA was induced using abscisic acid (ABA) but not with glucose, sucrose, or gibberellin treatment. To investigate the molecular mechanism underlying this effect, the ZmSSI promoter region (-1537 to +51) was isolated and analysed. A transient expression assay in maize endosperm tissue showed that the full-length ZmSSI promoter is activated by ABA. The results of deletion and mutation assays demonstrated that a CACCG motif in the ZmSSI promoter is responsible for the ABA inducibility. The results of binding shift assays indicated that this CACCG motif interacts with the maize ABI4 protein in vitro. The overexpression of ABI4 in endosperm tissue enhanced the activity of a promoter containing the CACCG motif in the absence of ABA treatment. Expression pattern analysis indicated that the transcription pattern of ABI4 in the developing maize endosperm was induced by ABA treatment but was only slightly affected by glucose or sucrose treatment. Taken together, these data indicate that ABI4 binds to the CACCG motif in the ZmSSI promoter and mediates its ABA inducibility.

  12. Examination of the "Theory of Guidance" in the View of 'Ali Ibn Abi Talib (A): An Exploration into the Nahj Al-Balaghah

    Rostami-Nasab, Abas Ali; Tajedini, Oranus; Sadatmoosavi, Ali


    This study examines the "Theory of Guidance" according to 'Ali ibn Abi Talib (a). This theory is based on three divine covenants or fundamentals in guidance including the divine Prophet, the divine Book, and the divine human nature ("fitrat"). Research in this regard seems essential because this theory has not been previously…

  13. Liming effects on the chemical composition of the organic surface layer of a mature Norway spruce stand (Picea abies [L.] Karst.)

    Rosenberg, W.; Nierop, K.G.J.; Knicker, H.; Jager, de P.A.; Kreutzer, K.; Weiá, T.


    The application of lime in a mature Norway spruce (Picea abies [L.] Karst.) forest in southern Germany induced major changes in the activity of soil organisms and root growth. Since this may influence the chemical compostion of the soil organic matter (SOM) of the organic surface layer, its composit

  14. Compuestos fenólicos y flavonoides como marcadores bioquímicos de la respuesta a estrés abiótico en plantas tolerantes



    Calatayud Vaello, P. (2010). Compuestos fenólicos y flavonoides como marcadores bioquímicos de la respuesta a estrés abiótico en plantas tolerantes. Archivo delegado

  15. Epidihydropinidine, the main piperidine alkaloid compound of Norway spruce (Picea abies) shows promising antibacterial and anti-Candida activity.

    Fyhrquist, Pia; Virjamo, Virpi; Hiltunen, Eveliina; Julkunen-Tiitto, Riitta


    This study reports for the first time promising antibacterial and antifungal effects of epidihydropinidine, the major piperidine alkaloid in the needles and bark of Norway spruce, Picea abies (L.) Karsten. Epidihydropinidine was growth inhibitory against all bacterial and fungal strains used in our investigation, showing the lowest MIC value of 5.37μg/mL against Pseudomonas aeruginosa, Enterococcus faecalis, Candida glabrata and C. albicans. Epidihydropinidine was nearly three times more active than tetracycline against P. aeruginosa and E. faecalis. Promising antibacterial effects were also recorded against Staphylococcus aureus and Bacillus cereus (MIC 10.75μg/mL) as well as against Salmonella enterica (MIC and MBC 43μg/mL). Our preliminary results suggest that epidihydropinidine as well related alkaloids of Norway spruce could be powerful candidates for new antibiotics and for preventing food spoilage.

  16. Variation in Mechanical Properties within Individual Annual Rings in the Resonance Spruce Wood (Picea abies (L. Karst.

    Waldemar Moliński


    Full Text Available This paper shows results of specific elastic modulus and specific tensile strength in the resonance spruce wood [Picea abies (L. Karst.] in relation to its heterogeneity within individual annual rings. It also presents changes in the average values of microfibril angle in S2 layer measured in tangential walls of tracheids (MFA within individual annual rings. On the grounds of the results, it can be concluded that specific modulus of elasticity and specific tensile strength of wood are strongly influenced by the position of the sample in annual rings. Generally, the values of these parameters increase along the width of annual rings. It is also noted that the specific modulus of elasticity and specific tensile strength depend on MFA. As MFA decreases, the values of these parameters increase.

  17. Discovery and molecular biology of the abortive infection phage resistance system AbiV from Lactococcus lactis

    Haaber, Jakob Brandt Borup


    Bakteriofager (fager) er bakteriedræbende virus som fungerer ved at sprøjte deres DNA ind i en bakteriecelle og udnytte dennes celle-apparat til at danne nye fager. Disse frigives til omgivelserne under ødelæggelse af bakteriens cellevæg hvorved cellen dør. Fager forårsager stor skade i industrier...... evolutionen har bakterier udviklet naturlige anti-fag barrierer som beskyttelse mod fag-angreb. En af disse såkaldte fag-resistensmekanismer hedder abortiv infektion (Abi) og den virker ved at standse fag-angrebet efter fagens DNA er kommet ind i cellen. Den enkelte celle dør herved, men bakteriepopulationen...

  18. Tree ring variability and climate response of Abies spectabilis along an elevation gradient in Mustang, Nepal

    Kharal, D.K.; Meilby, Henrik; Rayamajhi, S.;


    In mountainous areas including the Himalayas, tree lines are expected to advance to higher altitudes due to global climate change affecting the distribution and growth of plant species. This study aimed at identifying the tree ring variability of Abies spectabilis (D. Don) and its response...... to the climate along an elevation gradient in the high Himalayas of central Nepal. Tree core samples were collected from four sites in Mustang district. All sites were located in the same valley and exposed to similar weather conditions. Out of 232 samples collected from the sites, Titi lower (2700 m), Titi......-elevation sites the correlation between pre-monsoon precipitation and tree growth was positive, and for the month of May this was statistically significant (ptemperature (March-June) was negatively correlated with precipitation and with tree growth at all sites, and at the upper elevation...

  19. ABI and TBI in diabetics%糖尿病病人的踝肱指数和趾肱指数

    陈若飞; 罗文军; 唐博


    目的探讨踝肱指数(ABI)、趾肱指数(TBI)在2型糖尿病(T2DM)病人中的变化、影响因素.方法 142例2型糖尿病病人和40例非糖尿病病人对照进行ABI与TBI测量.记录病人高血压、血脂异常、吸烟、是否行胰岛素治疗等病史,检测血糖、血脂,测量血压.根据ABI和临床资料分组,进行统计学分析.结果 T2DM组TBI低于对照组TBI (P<0.01).ABI<1.3组与ABI≥1.3组间ABI比较,差异有统计学意义(P<0.01), TBI比较差异无统计学意义.T2DM组中,ABI和TBI在有和无高血压病史、有和无血脂异常及有和无吸烟病史比较差异均有统计学意义(P<0.01).在对照组和T2DM未合并下肢动脉疾病组,ABI与TBI相关系数r分别为0.44(P<0.05)、0.32(P<0.01).T2DM组中,ABI与TBI的一致性检验P<0.05.结论 2型糖尿病病人的TBI较正常人低,ABI与TBI受年龄、糖尿病病程、高血压、血脂异常、吸烟等因素影响.ABI与TBI的相关性不高.

  20. Fungistatic efficiency of phenolic compounds in spruce (Picea abies L. Karst. ) and influence of SO[sub 2]. Fungistatische Wirksamkeit phenolischer Inhaltsstoffe der Fichte Picea abies (L. ) Karst. und Einfluss von SO[sub 2

    Beyer, U. (TU Dresden, Inst. fuer Forstbotanik und Forstzoologie, Tharandt (Germany)); Tesche, M. (TU Dresden, Inst. fuer Forstbotanik und Forstzoologie, Tharandt (Germany)); Heller, W. (GSF - Forschungszentrum fuer Umwelt und Gesundheit GmbH, Neuherberg (Germany). Inst. fuer Biochemische Pflanzenpathologie); Sandermann, H. (GSF - Forschungszentrum fuer Umwelt und Gesundheit GmbH, Neuherberg (Germany). Inst. fuer Biochemische Pflanzenpathologie)


    The paper deals with fungistatic phenols in Norway spruce (Picea abies). Phenolic substances were isolated from several tissues and chromatographed by TLC. Detected by bioassay using Cladosporium cucumerinum, the most fungistatic compound was isolated and identified by UV-, mass- and NMT-spectra as the stilbenglucoside isorhapontin. The highest amounts of isorhapontin were found in a 15-year-old spruce in the bark of root (1.3%) and stem (0.4%), and also in fine roots (0.3%). Sapwood contained between 0.001 and 0.01% of this stilbene (all values related to green weight). The brown-rot fungi Phaeolus schweinitzii and Fomitopsis pinicola showed a depression of growth related to the concentration of isorhapontin on malt agar. Growth reduction of 50% was reached at a concentration of 1.25 mM (F. pinicola) and 3 mM (P. schweinitzii). The white-rot fungi Trametes versicolor, Heterobasidion annosum, and Armillaria ostoyae were not inhibited. First investigation results 6 months after long-term SO[sub 2]-treatment (0.28-0.34 ppm) of cloned spruces showed a reduction of fungistatic stilbenes in fine roots and bark compared with untreated controls (indirect effect). (orig.)

  1. Medicinal plants and traditional healing practices in ehotile people, around the aby lagoon (eastern littoral of Côte d'Ivoire)

    Malan, Djah F; Neuba, Danho F R; Kouakou, Kouakou L


    Background Access to useful plants is a growing problem in Africa, increased by the loss of natural vegetation and the erosion of traditional knowledge. Ethnobotany contributes to promote these indigenous knowledge. Despite the large diversity of ethnic groups in Côte d’Ivoire, few ethnomedicine researches have targeted these groups. Among the great Akan group, the Ehotile people are one of the smallest and oldest ethnic group around the Aby Lagoon. The goal of this study was to analyze the l...

  2. The effects of heartwood and sapwood on kraft pulp properties of Pinus nigra J.F.Arnold and Abies bornmuelleriana Mattf.

    Ataç, Yasin; EROĞLU, Hüdaverdi


    The effects of heartwood and sapwood on kraft pulp properties of Pinus nigra J.F.Arnold. and Abies bornmuelleriana Mattf. were investigated. The differences in terms of chemical composition and fiber properties between the heartwood and sapwood of these species were also examined. Heartwood had more holocellulose and extractive compared to sapwood. Moreover, heartwood fiber length was shorter than that of sapwood. Kraft cookings of heartwood and sapwood each species were separately done under...

  3. Impact of soil drainage to the radial stem growth of Norway spruce (Picea Abies L. Karst. in peatland forests

    Klempířová Barbora


    Full Text Available Peatland Norway spruce (Picea abies L. Karst. forests represent very valuable ecosystems with considerable importance for nature conservation. However, a lot of peatland forests have been drained or used for opencast mining of peat. Since dendrochronological and dendroecological studies on trees growing on peatlands in Europe are not many, this study aimed to reconstruct the impact of drainage to the growth of trees in forest stands older than 100 years in the moment of drainage. Dendrochronological analysis was performed on two 0.25-ha square sampling plots (50*50 m in two pre-selected stands (control site vs. drained site with similar natural conditions and age. The mean-value functions of the ring indices, comparing the drained site with the control site, in the period after 1940 revealed very similar radial-growth trends. After the year 1992, when one site was substantially drained, the radial-growth trends not showed any significant change. Likewise, the result of the independent two sample t-test for the period after 1992 has not revealed any substantial statistically important difference in the mean index between the control site and the drained site.

  4. Wood density variations of Norway spruce (Picea abies (L. Karst. under contrasting climate conditions in southwestern Germany

    Marieke van der Maaten-Theunissen


    Full Text Available We analyzed inter-annual variations in ring width and maximum wood density of Norway spruce (Picea abies (L. Karst. at different altitudes in Baden-Württemberg, southwestern Germany, to determine the climate response of these parameters under contrasting climate conditions. In addition, we compared maximum, average and minimum wood density between sites. Bootstrapped correlation coefficients of ring width and maximum wood density with monthly temperature and precipitation, revealed a different climate sensitivity of both parameters. Ring width showed strong correlations with climate variables in the previous year and in the first half of the growing season. Further, a negative relationship with summer temperature was observed at the low-altitude sites. Maximum wood density correlated best with temperature during the growing season, whereby strongest correlations were found between September temperature and maximum wood density at the high-altitude sites. Observed differences in maximum, average and minimum wood density are suggested to relate to the local climate; with lower temperatures and higher water availability having a negative effect on wood density. 

  5. Antimicrobial activities of several parts of Pinus brutia, Juniperus oxycedrus, Abies cilicia, Cedrus libani and Pinus nigra.

    Diğrak, M; Ilçim, A; Hakki Alma, M


    In this study, the antimicrobial activities of several parts of various trees grown in the Kahramanmaraş region of Turkey were investigated by the disc diffusion method. Chloroform, acetone and methanol extracts of leaves, resins, barks, cones and fruits of Pinus brutia Ten., Juniperus oxycedrus L., Abies cilicia Ant. & Kotschy Carr., Cedrus libani A. Rich. and Pinus nigra Arn. were prepared and tested against Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, Bacillus cereus FMC 19, Escherichia coli DM, Klebsiella pneumoniae FMC 3, Enterobacter aerogenes CCM 2531, Staphylococcus aureus Cowan 1, Mycobacterium smegmatis RUT, Proteus vulgaris FMC 1, Listeria monocytogenes Scoot A, Pseudomonas aeruginosa DSM 5007, Candida albicans CCM 314, Candida tropicalis MDC 86 and Penicillium italicum K. The results showed that antifungal effects were not observed for the whole extracts, E. coli was not inhibited by any of the plant extracts except by the chloroform and acetone extracts of the leaves of A. cilicia, which showed inhibition zones of 16-18 mm, respectively. All the plant extracts used in this study inhibited the development of the other bacteria studied. When the results of this study were compared with an ampicillin standard, it was found that the microorganisms studied were generally susceptible, intermediate or resistant to the extracts of species when compared with the ampicillin standard. On the other hand, the acetone and methanol extracts of Juniperus fruits were found to be quite resistant.

  6. Comparison of the Calibration Algorithms and SI Traceability of MODIS, VIIRS, GOES, and GOES-R ABI Sensors

    Raju Datla


    Full Text Available The radiometric calibration equations for the thermal emissive bands (TEB and the reflective solar bands (RSB measurements of the earth scenes by the polar satellite sensors, (Terra and Aqua MODIS and Suomi NPP (VIIRS, and geostationary sensors, GOES Imager and the GOES-R Advanced Baseline Imager (ABI are analyzed towards calibration algorithm harmonization on the basis of SI traceability which is one of the goals of the NOAA National Calibration Center (NCC. One of the overarching goals of NCC is to provide knowledge base on the NOAA operational satellite sensors and recommend best practices for achieving SI traceability for the radiance measurements on-orbit. As such, the calibration methodologies of these satellite optical sensors are reviewed in light of the recommended practice for radiometric calibration at the National Institute of Standards and Technology (NIST. The equivalence of some of the spectral bands in these sensors for their end products is presented. The operational and calibration features of the sensors for on-orbit observation of radiance are also compared in tabular form. This review is also to serve as a quick cross reference to researchers and analysts on how the observed signals from these sensors in space are converted to radiances.

  7. The Influence of Age, Location and Soil Conditions on the Allometry of Young Norway Spruce (Picea abies L. Karst. Trees

    Ioan DUTCA


    Full Text Available In this study the influence of tree’s age, location (i.e. latitude and altitude and soil conditions (i.e. pH, humus content, carbon to nitrogen ratio, cation exchange capacity and percent base saturation on tree allometry was investigated. The data was collected from 22 Norway spruce (Picea abies L. Karst plantations located in Eastern Carpathians of Romania, aged between 4 and 15. From each plantation a soil sample and 10 trees were collected for soil chemical properties and biomass measurements, respectively. Root collar diameter (RCD and height (H based allometric equations were developed for total tree and vegetative organs of the tree (i.e. stem, branches, needles and roots. Furthermore, the interaction between the standardised residuals of these models and the tested factors was analysed. In order to account for the random effect of the clustered data, the mixed-effect modelling procedure was used. The results have shown no influence of these factors (age, location and soil conditions on RCD based models, except for branches biomass model which was linked to soil carbon/nitrogen ratio. The H based models, however, were significantly influenced by latitude and soil cation exchange capacity as a consequence of H/RCD ratio change with these factors. The trees were more likely to allocate more to height growth when growing in higher latitudes or on soils with higher values of cation exchange capacity.

  8. Norway spruce (Picea abies) laccases:Characterization of a laccase in a lignin-forming tissue culture

    Sanna Koutaniemi; Heli A Malmberg; Liisa K Simola; Teemu H Teeri; Anna Karkonen


    Secondarily thickened cel wal s of water-conducting vessels and tracheids and support-giving sclerenchyma cel s contain lignin that makes the cel wal s water impermeable and strong. To what extent laccases and peroxidases contribute to lignin biosynthesis in muro is under active evaluation. We performed an in silico study of Norway spruce (Picea abies (L.) Karst.) laccases utilizing available genomic data. As many as 292 laccase encoding sequences (genes, gene fragments, and pseudogenes) were detected in the spruce genome. Out of the 112 genes annotated as laccases, 79 are expressed at some level. We isolated five ful-length laccase cDNAs from developing xylem and an extracel ular lignin-forming cel culture of spruce. In addition, we purified and biochemical y characterized one culture medium laccase from the lignin-forming cel culture. This laccase has an acidic pH optimum (pH 3.8–4.2) for coniferyl alcohol oxidation. It has a high affinity to coniferyl alcohol with an apparent Km value of 3.5 mM;however, the laccase has a lower catalytic efficiency (Vmax/Km) for coniferyl alcohol oxidation compared with some purified culture medium peroxidases. The properties are discussed in the context of the information already known about laccases/coniferyl alcohol oxidases of coniferous plants.

  9. Influence of Binasal and Uninasal Inhalations of Essential Oil of Abies koreana Twigs on Electroencephalographic Activity of Human

    Min Seo


    Full Text Available Objectives. The present work investigates the effect of essential oil from the twigs of Abies koreana on electroencephalographic (EEG activity of human brain in order to understand the influence of binasal and uninasal inhalations. Methods. To accomplish this study, the essential oil from the twigs of A. koreana (AEO was isolated by steam distillation and the EEG readings were recorded using QEEG-8 system from 8 grounding electrodes according to the International 10-20 System. Results. D-Limonene (25.29%, bornyl acetate (19.31%, camphene (12.48%, α-pinene (11.88%, β-pinene (6.45%, and eudesm-7(11-en-ol (5.38% were the major components in the essential oil. In the EEG study, the absolute alpha (left frontal and right parietal and absolute fast alpha (right parietal values significantly increased during the binasal inhalation of AEO. In the uninasal inhalation, absolute beta and theta values decreased significantly, especially in the right frontal and left and right parietal regions. The results revealed that the AEO produced different EEG power spectrum changes according to the nostril difference. Conclusion. The changes in EEG values due to the inhalation of AEO may contribute to the enhancement of relaxation (binasal inhalation and alertness/attention (right uninasal inhalation states of brain which could be used in aromatherapy treatments.

  10. ,* Copper transport and accumulation in spruce stems (picea abies(L.) Karsten) revelaed by laser-induced breakdown spectroscopy

    Krajcarova, Dr. Lucie [Czech Technical University; Novotny, Dr. Karel [Mendel University of Brno; Babula, Dr. Petr [Czech Technical University; Pravaznik, Dr Ivo [Czech Technical University; Kucerova, Dr. Petra [Czech Technical University; Vojtech, Dr. Adam [Czech Technical University; Martin, Madhavi Z [ORNL; Kizek, Dr. Rene [Czech Technical University; Kaiser, Jozef [ORNL


    Laser-Induced Breakdown Spectroscopy (LIBS) in double pulse configuration (DP LIBS) was used for scanning elemental spatial distribution in annual terminal stems of spruce (Picea abies (L.) Karsten). Cross sections of stems cultivated in Cu2+ solution of different concentrations were prepared and analyzed by DP LIBS. Raster scanning with 150 m spatial resolution was set and 2D (2-dimentional) maps of Cu and Ca distribution were created on the basis of the data obtained. Stem parts originating in the vicinity of the implementation of the cross sections were mineralized and subsequently Cu and Ca contents were analyzed by inductively coupled plasma mass spectrometry (ICP-MS). The results provide quantitative information about overall concentration of the elements in places, where LIBS measurements were performed. The fluorescence pictures were created to compare LIBS distribution maps and the fluorescence intensity (or the increase in autofluorescence) was used for the comparison of ICP-MS quantitative results. Results from these three methods can be utilized for quantitative measurements of copper ions transport in different plant compartments in dependence on the concentration of cultivation medium and/or the time of cultivation.

  11. Transcript Accumulation Dynamics of Phenylpropanoid Pathway Genes in the Maturing Xylem and Phloem of Picea abies during Latewood Formation

    Giovanni Emiliani; Maria Laura Traversi; Monica Anichini; Guido Giachi; Aiessio Giovannelli


    In temperate regions,latewood is produced when cambial activity declines with the approach of autumnal dormancy.The understanding of the temporal (cambium activity vs dormancy) and spatial (phloem,cambial region,maturing xylem) regulation of key genes involved in the phenylpropanoid pathway during latewood formation represents a crucial step towards providing new insights into the molecular basis of xylogenesis.In this study,the temporal pattern of transcript accumulation of 12 phenylpropanoid genes (PAL1,C4H3I5,C4H4,4CL3,4CL4,HCT1,C3H3,CCoAOMT1,COMT2,COMT5,CCR2) was analyzed in maturing xylem and phloem of Picea abies during latewood formation.Quantitative reverse transcription-polymerase chain reaction analyses revealed a well-defined RNA accumulation pattern of genes involved in the phenylpropanoid pathway during latewood formation.Differences in the RNA accumulation patterns were detected between the different tissue types analyzed.The results obtained here demonstrated that the molecular processes involved in monolignol biosynthesis are not restricted to the cambial activity timeframe but continued after the end of cambium cell proliferation.Furthermore,since it has been shown that lignification of maturing xylem takes place in late autumn,we argue on the basis of our data that phloem could play a key role in the monolignol biosynthesis process.

  12. Effect of bark beetle (Ips typographus L.) attack on bark VOC emissions of Norway spruce (Picea abies Karst.) trees

    Ghimire, Rajendra P.; Kivimäenpää, Minna; Blomqvist, Minna; Holopainen, Toini; Lyytikäinen-Saarenmaa, Päivi; Holopainen, Jarmo K.


    Climate warming driven storms are evident causes for an outbreak of the European spruce bark beetle (Ips typographus L.) resulting in the serious destruction of mature Norway spruce (Picea abies Karst.) forests in northern Europe. Conifer species are major sources of biogenic volatile organic compounds (BVOCs) in the boreal zone. Climate relevant BVOC emissions are expected to increase when conifer trees defend against bark beetle attack by monoterpene (MT)-rich resin flow. In this study, BVOC emission rates from the bark surface of beetle-attacked and non-attacked spruce trees were measured from two outbreak areas, Iitti and Lahti in southern Finland, and from one control site at Kuopio in central Finland. Beetle attack increased emissions of total MTs 20-fold at Iitti compared to Kuopio, but decreased the emissions of several sesquiterpenes (SQTs) at Iitti. At the Lahti site, the emission rate of α-pinene was positively correlated with mean trap catch of bark beetles. The responsive individual MTs were tricyclene, α-pinene, camphene, myrcene, limonene, 1,8-cineole and bornyl acetate in both of the outbreak areas. Our results suggest that bark beetle outbreaks affect local BVOC emissions from conifer forests dominated by Norway spruce. Therefore, the impacts of insect outbreaks are worth of consideration to global BVOC emission models.

  13. Comportamiento en flexión estática de vigas de madera antigua de Picea abies

    Javier Ramón Sotomayor-Castellanos


    Full Text Available Las tipologías arquitecturales encontradas en el contexto del análisis estructural de construcciones con significado histórico y cultural son complejas. El modelado numérico es propuesto como una solución para diagnosticar la seguridad estructural de estas edificaciones. En esta investigación se realizaron pruebas de flexión estática de acuerdo a la norma Europea EN 408 y se calcularon los módulos de elasticidad y de ruptura en 5 vigas antiguas de Picea abies. La densidad de la madera fue de 422 kg/m3 para las vigas con un contenido de humedad de 12 %. El módulo de elasticidad promedio fue de 9,059 MPa y el módulo de ruptura fue de 29.18 Pa. Los módulos presentaron una variabilidad aceptable. Los resultados experimentales se contrastaron con las predicciones numéricas empleando el método del elemento finito. Las predicciones numéricas en los dominios lineal y no lineal mostraron buena aproximación con los resultados experimentales.

  14. Good manufacturing practice production of [(68)Ga]Ga-ABY-025 for HER2 specific breast cancer imaging.

    Velikyan, Irina; Wennborg, Anders; Feldwisch, Joachim; Lindman, Henrik; Carlsson, Jörgen; Sörensen, Jens


    Therapies targeting human epidermal growth factor receptor type 2 (HER2) have revolutionized breast cancer treatment, but require invasive biopsies and rigorous histopathology for optimal patient stratification. A non-invasive and quantitative diagnostic method such as positron emission tomography (PET) for the pre-therapeutic determination of the presence and density of the HER2 would significantly improve patient management efficacy and treatment cost. The essential part of the PET methodology is the production of the radiopharmaceutical in compliance with good manufacturing practice (GMP). The use of generator produced positron emitting (68)Ga radionuclide would provide worldwide accessibility of the agent. GMP compliant, reliable and highly reproducible production of [(68)Ga]Ga-ABY-025 with control over the product peptide concentration and amount of radioactivity was accomplished within one hour. Two radiopharmaceuticals were developed differing in the total peptide content and were validated independently. The specific radioactivity could be kept similar throughout the study, and it was 6-fold higher for the low peptide content radiopharmaceutical. Intrapatient comparison of the two peptide doses allowed imaging optimization. The high peptide content decreased the uptake in healthy tissue, in particular liver, improving image contrast. The later imaging time points enhanced the contrast. The combination of high peptide content radiopharmaceutical and whole-body imaging at 2 hours post injection appeared to be optimal for routine clinical use.

  15. Volatile Profile of Croatian Lime Tree (Tilia sp., Fir Honeydew (Abies alba and Sage (Salvia officinalis Honey

    Olivera Koprivnjak


    Full Text Available Volatile profiles of lime tree (Tilia sp., fir honeydew (Abies alba and sage (Salvia officinalis honey produced in Croatia have been studied by using headspace solid phase microextraction (HS-SPME followed by gas chromatography-mass spectrometry (GC-MS analysis. Melissopalynological and sensory characterization have been performed in order to check the reliability of botanical origin of the samples. In case of sage honey, sensory characteristics are reported for the first time and are rather uniform including: colour characterized as beige to jade, depending on the consistency; odour characterized as between light and medium intensity, slightly pungent and wooden; taste characterized as low sweetness, expressive acidity and apple caramel, with persistent fruity aftertaste. Characteristic volatile profiles of the analyzed honeys are described. Taking into consideration similarities with lime and fir honey volatile profiles reported in literature, characteristic volatile compounds resulting from qualitative data evaluation are proposed. Sage honey volatile profile has been reported for the first time and it was found quite different compared to the other studied honeys showing the lowest number of peaks among the studied honeys, 34. Several compounds belonging to the sage honey volatile profile have been identified for the first time in honeys. They include tetrahydro-2,2,5,5-tetramethylfuran, 3-hexenyl ester of butanoic acid, 2-methylbenzene, maltol, methyl ester of 3-furanocarboxylic acid and benzeneacetic acid. Based on the obtained results and with the lack of comparative literature data, they are proposed as characteristic volatiles for the volatile pattern of sage honey.

  16. Isoprene and monoterpene emission from the coniferous species Abies Borisii-regis - implications for regional air chemistry in Greece

    Harrison, D.; Hunter, M.C.; Lewis, A.C.; Seakins, P.W. [Leeds Univ., School of Chemistry, Leeds (United Kingdom); Nunes, T.V.; Pio, C.A. [Universidade de Aveiro, Dept. de Ambiente e Ordenamento, Aveiro (Portugal)


    The emission of isoprene has been studied from a forest of Abies Borisii-regis, a Mediterranean fir species previously thought to emit only monoterpenes. Emission studies from two independent enclosure experiments indicated a standardised isoprene emission rate of (18.4{+-}3.8){mu}gg{sub dry-weight} {sup -1}h{sup -1}, similar in magnitude to species such as eucalyptus and oak which are considered to be strong isoprene emitters. Isoprene emission depended strongly on both leaf temperature (2degC-34degC) and photosynthetically active radiation (PAR) below 250{mu}molm{sup -2}s{sup -1}, becoming saturated with respect to PAR above this value. The annual isoprene emission rate was estimated to be (132{+-}29) kTyr{sup -1} for those trees growing within Greece, comparable to current estimates of the total isoprene budget of Greece as a whole, and contributing significantly to regional ozone and carbon monoxide budgets. Monoterpene emission exhibited exponential temperature dependence, with 1,8-cineole, {alpha}-pinene, {beta}-pinene and limonene forming the primary emissions. A standardised total monoterpene emission rate of (2.7{+-}1.1){mu}gg{sub dry-weight} {sup -1}h{sup -1} was calculated, corresponding to an annual monoterpene emission rate of (24{+-}12) kTyr{sup -1}. Research was conducted as part of the AEROBIC'97 (AEROsol formation from Biogenic organic Carbon) series of field campaigns. (Author)

  17. Mutational analysis of a monoterpene synthase reaction: altered catalysis through directed mutagenesis of (-)-pinene synthase from Abies grandis.

    Hyatt, David C; Croteau, Rodney


    Two monoterpene synthases, (-)-pinene synthase and (-)-camphene synthase, from grand fir (Abies grandis) produce different product mixtures despite having highly homologous amino acid sequences and, presumably, very similar three-dimensional structures. The major product of (-)-camphene synthase, (-)-camphene, and the major products of (-)-pinene synthase, (-)-alpha-pinene, and (-)-beta-pinene, arise through distinct mechanistic variations of the electrophilic reaction cascade that is common to terpenoid synthases. Structural modeling followed by directed mutagenesis in (-)-pinene synthase was used to replace selected amino acid residues with the corresponding residues from (-)-camphene synthase in an effort to identify the amino acids responsible for the catalytic differences. This approach produced an enzyme in which more than half of the product was channeled through an alternative pathway. It was also shown that several (-)-pinene synthase to (-)-camphene synthase amino acid substitutions were necessary before catalysis was significantly altered. The data support a model in which the collective action of many key amino acids, located both in and distant from the active site pocket, regulate the course of the electrophilic reaction cascade.

  18. Effects of disturbance on regeneration of Abies fabri forests at the upper reaches of the Yangtze River

    Gao Jia-rong; Gao Yang


    Mudflow is the principal disturbance in Abies fabri forests. In the Gongga Mountain areas of the upper reaches of the Yangtze River, the intensities and periodicity of different scale mudflows vary. Small-scale mudflows are more frequent, occurring every one or two years while large-scale mudflows may occur once in more than one hundred years. Through a field study of A. fabri forests during different stages of growth, we analyzed their structural characteristics and discovered that after different sizes of mudflow, poplar and birch often occupy the dominant canopy at the expense of the slow growing A. fabri, for only a small number of saplings are A. fabri that occurs in the first regeneration stage. However, a large number of seed resources can be found in mature A.fabri forests and as a unique regeneration species, A. fabri will gradually replace all the other species and form a stable community of strong shade-tolerant trees. Because of the intimate relationship between growing conditions and soil and water conservation at the upper reaches of the Yangtze River, we should carry out some artificial measurements to control and promote the slow regeneration process of A. fabri.

  19. Influence of Binasal and Uninasal Inhalations of Essential Oil of Abies koreana Twigs on Electroencephalographic Activity of Human

    Seo, Min; Sowndhararajan, Kandhasamy


    Objectives. The present work investigates the effect of essential oil from the twigs of Abies koreana on electroencephalographic (EEG) activity of human brain in order to understand the influence of binasal and uninasal inhalations. Methods. To accomplish this study, the essential oil from the twigs of A. koreana (AEO) was isolated by steam distillation and the EEG readings were recorded using QEEG-8 system from 8 grounding electrodes according to the International 10-20 System. Results. D-Limonene (25.29%), bornyl acetate (19.31%), camphene (12.48%), α-pinene (11.88%), β-pinene (6.45%), and eudesm-7(11)-en-ol (5.38%) were the major components in the essential oil. In the EEG study, the absolute alpha (left frontal and right parietal) and absolute fast alpha (right parietal) values significantly increased during the binasal inhalation of AEO. In the uninasal inhalation, absolute beta and theta values decreased significantly, especially in the right frontal and left and right parietal regions. The results revealed that the AEO produced different EEG power spectrum changes according to the nostril difference. Conclusion. The changes in EEG values due to the inhalation of AEO may contribute to the enhancement of relaxation (binasal inhalation) and alertness/attention (right uninasal inhalation) states of brain which could be used in aromatherapy treatments. PMID:28042202

  20. Growth, allocation and tissue chemistry of Picea abies seedlings affected by nutrient supply during the second growing season.

    Kaakinen, Seija; Jolkkonen, Annika; Iivonen, Sari; Vapaavuori, Elina


    One-year-old Norway spruce (Picea abies (L.) Karst.) seedlings were grown hydroponically in a growth chamber to investigate the effects of low and high nutrient availability (LN; 0.25 mM N and HN; 2.50 mM N) on growth, biomass allocation and chemical composition of needles, stem and roots during the second growing season. Climatic conditions in the growth chamber simulated the mean growing season from May to early October in Flakaliden, northern Sweden. In the latter half of the growing season, biomass allocation changed in response to nutrient availability: increased root growth and decreased shoot growth led to higher root/shoot ratios in LN seedlings than in HN seedlings. At high nutrient availability, total biomass, especially stem biomass, increased, as did total nonstructural carbohydrate and nitrogen contents per seedling. Responses of stem chemistry to nutrient addition differed from those of adult trees of the same provenance. In HN seedlings, concentrations of alpha-cellulose, hemicellulose and lignin decreased in the secondary xylem. Our results illustrate the significance of retranslocation of stored nutrients to support new growth early in the season when root growth and nutrient uptake are still low. We conclude that nutrient availability alters allocation patterns, thereby influencing the success of 2-year-old Norway spruce seedlings at forest planting sites.

  1. Arabidopsis HOOKLESS1 Regulates Responses to Pathogens and Abscisic Acid through Interaction with MED18 and Acetylation of WRKY33 and ABI5 Chromatin.

    Liao, Chao-Jan; Lai, Zhibing; Lee, Sanghun; Yun, Dae-Jin; Mengiste, Tesfaye


    Arabidopsis thaliana HOOKLESS1 (HLS1) encodes a putative histone acetyltransferase with known functions in seedling growth. Here, we show that HLS1 regulates plant responses to pathogens and abscisic acid (ABA) through histone acetylation at chromatin of target loci. The hls1 mutants show impaired responses to bacterial and fungal infection, accelerated senescence, and impaired responses to ABA. HLS1 modulates the expression of WRKY33 and ABA INSENSITIVE5 (ABI5), known regulators of pathogen and ABA responses, respectively, through direct association with these loci. Histone 3 acetylation (H3Ac), a positive mark of transcription, at WRKY33 and ABI5 requires HLS1 function. ABA treatment and pathogen infection enhance HLS1 recruitment and H3Ac at WRKY33. HLS1 associates with Mediator, a eukaryotic transcription coregulatory complex, through direct interaction with mediator subunit 18 (MED18), with which it shares multiple functions. HLS1 recruits MED18 to the WRKY33 promoter, boosting WKRY33 expression, suggesting the synergetic action of HLS1 and MED18. By contrast, MED18 recruitment to ABI5 and transcriptional activation are independent of HLS1. ABA-mediated priming of resistance to fungal infection was abrogated in hls1 and wrky33 mutants but correlated with ABA-induced HLS1 accumulation. In sum, HLS1 provides a regulatory node in pathogen and hormone response pathways through interaction with the Mediator complex and important transcription factors.

  2. The influence of O3, NO2 and SO2 on growth of Picea abies and Fagus sylvatica in the Carpathian Mountains.

    Muzika, R M; Guyette, R P; Zielonka, T; Liebhold, A M


    At 17 long-term pollution monitoring sites throughout the Carpathian Mountains, tree growth patterns and variation in growth rate were examined to determine relationship of tree growth to specific pollutants. Canopy dominant Picea abies and Fagus sylvatica were selected at each site. Basal area increment (BAI) values were calculated from raw ring widths and used as an estimate of tree growth. Across all sites, BAI chronologies were highly variable, therefore local conditions and forest structure accounted for considerable variation. Several significant relationships, however, implicated a role of pollutants on tree growth. Average levels (1997-1999) of NO(2) and SO(2) were inversely related to BAI means (1989-1999). Although average O(3) alone was not related to growth, the maximum O(3) value reported at the sites was negatively correlated with overall growth. A variable representing the combined effect of O(3), NO(2) and SO(2) was negatively correlated with both P. abies and F. sylvatica growth. Pollution data were used to categorize all sites into 'high' or 'low' pollution sites. Difference chronologies based on these categories indicated trends of decline in the 'high' pollution sites relative to 'low' pollution site. In the more heavily polluted sites, the BAI of Fagus sylvatica has declined approximately 50% and Picea abies has declined 20% over the past 45 years.

  3. Evaluating the usability of a single UK community acquired brain injury (ABI) rehabilitation service website: implications for research methodology and website design.

    Newby, Gavin; Groom, Christina


    Information provision is an important resource for those living with acquired brain injury (ABI) and their families. Web-based health information services are now common additions to health service provision. Ideally, they should be easy to use and provide useful, relevant and accurate information. ABI injuries do not affect individuals in the same way, and survivors can have a wide range of abilities and impairments. Therefore, any informational resource intended for this group should take account of their needs and help to compensate for their limitations. This pilot study recruited a group of individuals with ABI (of a median Extended Glasgow Outcome Scale rating of "lower moderate disability") who were clients of a UK National Health Service rehabilitation service and asked them to assess a specialised website provided by that service and hosted by their employing Primary Care Trust organisation. Participants completed a practical task and then gave their opinions on various aspects of website design, and content. They were also asked to suggest improvements and recommend additions. Overall the results were favourable. However, improvements in the legibility, layout and writing style were identified. There were also requests to add more information on the existing topics and add additional topics. The discussion also evaluates the utility of the methodology and the implications of the results for others considering constructing their own website.

  4. 也门1区块Abyed-2井卡钻事故的处理%Handling of Pipe Stuck in Well Abyed-2,Block 1 of Yemen

    张君亚; 夏柏如; 赵增新; 王长生; 崔卫华; 赵胜英



  5. Tree water status and growth of saplings and mature Norway spruce (Picea abies at a dry distribution limit

    Walter eOberhuber


    Full Text Available We evaluated the size effect on stem water status and growth in Norway spruce (Picea abies (L. Karst. occurring at the edge of its natural range in a dry inner Alpine environment (750 m asl, Tyrol, Austria. Intra-annual dynamics of stem water deficit (ΔW, maximum daily shrinkage (MDS and radial growth (RG were compared among saplings (stem diameter/height: 2.2 cm/93 cm; n = 7 and mature adult trees (25 cm/12.7 m; n = 6 during 2014. ΔW, MDS and RG were extracted from stem diameter variations, which were continuously recorded by automatic dendrometers and the influence of environmental drivers was evaluated by applying moving correlation analysis (MCA. Additionally, we used Morlet wavelet analysis to assess the differences in cyclic radial stem variations between saplings and mature trees. Results indicate that saplings and mature trees were experiencing water limitation throughout the growing season. However, saplings exhibited a more strained stem water status and higher sensitivity to environmental conditions than mature trees. Hence, the significantly lower radial increments in saplings (0.16 ± 0.03 mm compared to mature trees (0.54 ± 0.14 mm is related to more constrained water status in the former, affecting the rate and duration of RG. The wavelet analysis consistently revealed more distinct diurnal stem variations in saplings compared to mature trees. Intra-annual RG was most closely related to climate variables that influence transpiration, i.e., vapor pressure deficit, relative air humidity, and air temperature. MCA, however, showed pronounced instability of climate-growth relationships, which masked missing temporal or significant correlations when the entire study period (April-October was considered. We conclude that an increase in evaporative demand will impair regeneration and long-term stability of drought-prone inner Alpine Norway spruce forests.

  6. Carbon dioxide concentration and nitrogen input affect the C and N storage pools in Amanita muscaria-Picea abies mycorrhizae.

    Turnau, K; Berger, A; Loewe, A; Einig, W; Hampp, R; Chalot, M; Dizengremel, P; Kottke, I


    We studied the influence of elevated atmospheric CO2 concentration ([CO2]) on the vacuolar storage pool of nitrogen-containing compounds and on the glycogen pool in the hyphal sheath of Amanita muscaria (L. ex Fr.) Hooker-Picea abies L. Karst. mycorrhizae grown with two concentrations of ammonium in the substrate. Mycorrhizal seedlings were grown in petri dishes on agar containing 5.3 or 53 mg N l(-1) and exposed to 350 or 700 microl CO2 l(-1) for 5 or 7 weeks, respectively. Numbers and area of nitrogen-containing bodies in the vacuoles of the mycorrhizal fungus were determined by light microscopy linked to an image analysis system. The relative concentration of nitrogen in the vacuolar bodies was measured by electron energy loss spectroscopy (EELS). Glycogen stored in the cytosol was determined at the ultrastructural level by image analysis after staining the sections (PATAg test). Shoot dry weight, net photosynthesis and relative amounts of N in vacuolar bodies were greater at the higher N and CO2 concentrations. The numbers and areas of vacuolar N-containing bodies were significantly greater at the higher N concentration only at ambient [CO2]. In the same treatment the percentage of hyphae containing glycogen declined to nearly zero. We conclude that, in the high N/low [CO2] treatment, the mycorrhizal fungus had an insufficient carbohydrate supply, partly because of increased amino acid synthesis by the non-mycorrhizal rootlets. When [CO2] was increased, the equilibrium between storage of glycogen and N-containing compounds was reestablished.

  7. Influence of low- and high-elevation plant genomes on the regulation of autumn cold acclimation in Abies sachalinensis

    Wataru eIshizuka


    Full Text Available Boreal coniferous species with wide geographic distributions show substantial variation in autumn cold acclimation among populations. To determine how this variation is inherited across generations, we conducted a progeny test and examined the development of cold hardening in open-pollinated second-generation (F2 progeny of Abies sachalinensis. The F1 parents had different genetic backgrounds resulting from reciprocal interpopulational crosses between low-elevation (L and high-elevation (H populations: L × L, L × H, H × L, and H × H. Paternity analysis of the F2 progeny using molecular genetic markers showed that 91.3% of the fathers were located in surrounding stands of the F1 planting site (i.e., not in the F1 test population. The remaining fathers were assigned to F1 parents of the L × L cross-type. This indicates that the high-elevation genome in the F1 parents was not inherited by the F2 population via pollen flow. The timing of autumn cold acclimation in the F2 progeny depended on the cross-type of the F1 mother. The progeny of H × H mothers showed less damage in freezing tests than the progeny of other cross-types. Statistical modeling supported a linear effect of genome origin. In the best model, variation in freezing damage was explained by the proportion of maternally inherited high-elevation genome. These results suggest that autumn cold acclimation was partly explained by the additive effect of the responsible maternal genome. Thus, the offspring that inherited a greater proportion of the high-elevation genome developed cold hardiness earlier. Genome-based variation in the regulation of autumn cold acclimation matched the local climatic conditions, which may be a key factor in elevation-dependent adaptation.

  8. Secondary metabolites of the lichen Hypogymnia physodes (L.) Nyl. and their presence in spruce (Picea abies (L.) H. Karst.) bark.

    Latkowska, Ewa; Bober, Beata; Chrapusta, Ewelina; Adamski, Michal; Kaminski, Ariel; Bialczyk, Jan


    Lichen species typically have a characteristic profile of secondary metabolites. Dense populations of Hypogymnia physodes growing frequently as epiphytes on tree branches have harmful effects on the host, likely due to their secondary compounds, which were undetected in tree tissues until now. The aim of the present study was to re-characterise the suite of secondary metabolites of H. physodes thalli and to estimate their translocation into spruce (Picea abies) bark. Thallus and bark extracts were compared using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The compounds were identified based on their UV, MS and MS/MS spectra as well as retention factors of their TLC analysis. In addition to the previously described secondary metabolites (protocetraric, physodalic, 3-hydroxyphysodic, physodic, and 2'-O-methylphysodic acids, atranorin and chloroatranorin) of H. physodes, further three were identified in its thalli: conphysodalic, 4-O-methylphysodic and α-alectoronic acids. Fragmentation patterns from the negative ionisation of each compound were proposed, some of which were described for the first time. Among all of the detected lichen substances, a few, e.g., physodalic, 3-hydroxyphysodic, physodic acids and atranorin, were present in the bark of spruce branches that were abundantly colonised by lichen. The newly identified compounds of H. physodes thalli may belong to its constant or accessory secondary metabolites. These compounds may be useful in the chemotaxonomic classification of this species. The presence of some lichen substances in spruce bark confirmed their ability to penetrate host tissues. These data suggest that H. physodes compounds may cause long-term effects on spruces in nature.

  9. Effects of acidic fog on seedlings of Pinus ponderosa and Abies concolor: foliar injury, physiological and biochemical responses.

    Takemoto, B K; Bytnerowicz, A


    Seedlings of Pinus ponderosa (ponderosa pine) and Abies concolor (white fir) were exposed to acidic fog (pH 2.0, 3.0 or 4.0) in open-field plots for six weeks. The two species exhibited dissimilar injury responses; neither current year nor previous year needles of ponderosa pine were injured by pH 2.0 fog, but current year needles exhibited higher membrane permeability responses (i.e. needle extract conductivity, K+ concentration). In comparison, both needle age classes in white fir were significantly injured by pH 2.0 fog, but no significant effects on membrane permeability were observed. For both species, whole-study average rates of net photosynthesis in previous year needles were lower in plants exposed to pH 2.0 fog than in plants treated with pH 4.0 fog. While decreased process rates coincided with leaf necrosis in white fir, stomatal closure appeared to be the mechanism of inhibition in ponderosa pine with pH 2.0 fog (i.e. no visible injury). The findings of the present study provide evidence that frequent applications of highly acidic fog (i.e. pH 2.0-3.0) can cause temporal alterations in membrane permeability and gas exchange rates in western conifer seedlings, in the presence or absence of visible injury. However, because incipient effects on other measures of foliage health were species-specific (i.e. concentrations of starch, photosynthetic pigments, inorganic nutrients), a general mechanism of phytotoxicity could not be identified.

  10. Variation in spread of Heterobasidion annosum in clones of Picea abies grown at different vegetation phases under greenhouse conditions

    Svedjemark, G.; Stenlid, J. [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Mycology and Pathology


    Forty-nine Picea abies (L.) Karst clones were inoculated under greenhouse conditions with a Heterobasidion annosum (Fr.) Bref. isolate of the S intersterility group. The cuttings were inoculated at the following vegetation stages; bud-flushing stage, vegetative stage and after bud-set. Fungal growth in sapwood and leison length in the inner bark were measured after 34 days. The susceptibility of the various clones to H. annosum was strongly correlated among the three vegetation stages, both in terms of mean growth and mean growth ranking. Partitioning of variance components showed that variation in growth was explained by physiological stages and clone to 4% and 24%, respectively, and for interaction between clone and physiological stage to 9%. Corresponding values for leison length in the inner bark were 3%, 14% and 5%, respectively. Fungal growth in wood and leison length in the inner bark were strongly correlated (r{sup 2} ranging between 0.23 and 0.36). When cuttings were inoculated during bud-flushing, leison length and fungal growth in wood were both strongly correlated with bud-flushing index of the cuttings (r{sup 2} = 0.03 and 0.04 respectively) but that was not the case for the other stages. The number of active fine-roots and the degree of wilting of the cuttings were negatively correlated with leison length and fungal growth (r{sup 2} ranging between 0.01 and 0.13). Height and diameter varied greatly between the clones and both were negatively correlated with fungal extension (r{sup 2} ranging between 0.01 and 0.09). 33 refs, 2 figs, 3 tabs

  11. [Spatial point pattern analysis of Abies georgei var. smithii in forest of Sygera Mountains in southeast Tibet, China].

    Xie, Chuan-qi; Tian, Min-xia; Zhao, Zhong-rui; Zheng, Wei-lie; Wang, Guo-yan


    In this study, based on a 4 hm2 stem-mapping plot, we analyzed spatial distributions of Abies georgei var. smithii, the dominant species in forest of Sygera Mountains in southeast Tibet, China. Pair-correlation function was used to characterize univariate spatial point patterns of three size classes of the population and bivariate spatial patterns between those and different sizes of dead wood. A. georgei var. smithii population was characterized by reverse J-shaped DBH distribution, indicating an increasing population. Saplings of the population were spatially obviously aggregated at the small scales (0-7 m), and mid-sized trees and large-sized trees of the population were randomly or uniformly distributed. The aggregation intensities of A. georgei var. smithii decreased with the increasing diameter classes and spatial scales. Saplings and mid-sized trees were significantly and negatively associated with large-sized trees at the small scales (0-35 and 0-30 m), but the associations reversed at the large scales (45-100 and 80-100 m). In addition, with the increasing age difference between diameter classes of the population, the intensities of positive or negative correlations increased. Spatial associations between saplings and dead large-sized trees, and between mid-sized trees and dead large-sized trees were negative at the small scales (0-34 and 5-27 m), but positive at the large scales (49-100 and 73-100 m). This suggested that released niche space due to dead large-sized trees is not enough to weaken their negative impacts on saplings. We concluded that self-thinning effect and Janzen-Connell hypothesis may be the main mechanisms for the spatial pattern formation of A. georgei var. smithii population.

  12. Clavulina-Membranomyces is the most important lineage within the highly diverse ectomycorrhizal fungal community of Abies religiosa.

    Argüelles-Moyao, Andrés; Garibay-Orijel, Roberto; Márquez-Valdelamar, Laura Margarita; Arellano-Torres, Elsa


    Abies religiosa is an endemic conifer of Mexico, where its monodominant forests are the winter refuge of the monarch butterfly. Due to climate change, it has been estimated that by 2090, A. religiosa populations will decline by 96.5 %. To achieve success, reforestation programs should consider its ectomycorrhizal (ECM) fungi. We used ITS nrDNA sequences to identify the ECM fungi associated with A. religiosa and, based on its abundance and frequency, determined the diversity and community structure in a pure A. religiosa forest near Mexico City. Using sequence metadata, we inferred the species geographic distribution and host preferences. We conducted phylogenetic analyses of the Clavulinaceae (the most important family). The ECM community held 83 species, among which the richest genera were Inocybe (21 species), Tomentella (10 species), and Russula (8 species). Besides its low species richness, the Clavulina-Membranomyces lineage was the most dominant family. Clavulina cf. cinerea and Membranomyces sp. exhibited the highest relative abundance and relative frequency values. Phylogenetic analyses placed the Clavulinaceae genotypes in three different clades: one within Membranomyces and two within Clavulina. A meta-analysis showed that the majority of the ECM fungi (45.78 %) associated with A. religiosa in Mexico have also been sequenced from North America and are shared by Pinaceae and Fagaceae. In contrast, because they have not been sequenced previously, 32.2 % of the species have a restricted distribution. Here, we highlight the emerging pattern that the Clavulina-Membranomyces lineage is dominant in several ECM communities in the Neotropics, including Aldinia and Dicymbe legume tropical forests in the Guyana Shield, the Alnus acuminata subtropical communities, and the A. religiosa temperate forests in Mexico.

  13. SlSOM inhibits seed germination by regulating the expression of ABA/GA metabolic genes and SlABI5 in Solanum lycopersicum

    SUN Xiao-chun; GAO Yong-feng; ZHANG Ning; LI Hui-rong; YANG Shu-zhang; LIU Yong-sheng


    SOM encodes a nucleus-localized CCCH-type zinc finger protein and negatively regulates seed germination in Arabidopsis thaliana. We have previously demonstrated that ectopic expression of SlABI3, an important transcription factor in abscisic acid (ABA) signaling pathway, resulted in alteration of SlSOM expression patterns in both leaf and seed of tomato. In this study, we aimed to elucidate the function of tomato SlSOM in regarding to seed germination and seedling development. Here, we constructed SlSOM over-expression vector pBI121-SOM driven by CaMV 35S promoter, and the recombinant plasmid was incorporated into wild-type tomato by the method of Agrobacterium tumefaciens-mediated transformation. The result showed that over-expression of SlSOM conferred enhanced responses to exogenous ABA application during seed germination and seedling development. In addition, ectopic expression of SlSOM resulted in the alteration of expression level of ABA/GA (gibberel ins) metabolic genes, such as SlABA1, SlCYP707A1, SlGA3ox2, and SlGA2ox4, in both leaf and seed. The ABA anabolic gene SlABA1 and the GA catabolic gene SlGA2ox4 were up-regulated while the ABA catabolic gene SlCYP707A1 and the GA anabolic gene SlGA3ox2 were down-regulated. Compared to wild type, the expression level of SlABI5 was increased by about 40–50%in transgenic seeds while adding exogenous ABA treatment. These results support the notion that SlSOM inhibits seed germination by regulating ABA/GA metabolic genes and SlABI5 expression in Solanum lycopersicum.

  14. The influence of O{sub 3}, NO{sub 2} and SO{sub 2} on growth of Picea abies and Fagus sylvatica in the Carpathian Mountains

    Muzika, R.M.; Guyette, R.P.; Zielonka, T.; Liebhold, A.M


    Trends in tree growth rates can be explained by long-term effects of air pollution. - At 17 long-term pollution monitoring sites throughout the Carpathian Mountains, tree growth patterns and variation in growth rate were examined to determine relationship of tree growth to specific pollutants. Canopy dominant Picea abies and Fagus sylvatica were selected at each site. Basal area increment (BAI) values were calculated from raw ring widths and used as an estimate of tree growth. Across all sites, BAI chronologies were highly variable, therefore local conditions and forest structure accounted for considerable variation. Several significant relationships, however, implicated a role of pollutants on tree growth. Average levels (1997-1999) of NO{sub 2} and SO{sub 2} were inversely related to BAI means (1989-1999). Although average O{sub 3} alone was not related to growth, the maximum O{sub 3} value reported at the sites was negatively correlated with overall growth. A variable representing the combined effect of O{sub 3}, NO{sub 2} and SO{sub 2} was negatively correlated with both P. abies and F. sylvatica growth. Pollution data were used to categorize all sites into 'high' or 'low' pollution sites. Difference chronologies based on these categories indicated trends of decline in the 'high' pollution sites relative to 'low' pollution site. In the more heavily polluted sites, the BAI of Fagus sylvatica has declined approximately 50% and Picea abies has declined 20% over the past 45 years.

  15. 拟南芥PKS5激酶磷酸化ABI5参与植物ABA响应

    赵菲佚; 焦成瑾; 陈荃; 王太术; 田春芳; 高雅梅


    植物激素脱落酸(abscisic acid,ABA)在植物生长、发育及环境胁迫中起着重要的作用。本研究发现拟南芥PKSes(SOS2-like protein kinases)蛋白激酶家族成员PKS5(SOS2-like protein kinase 5)参与植物ABA响应。PKS5功能获得性点突变体pks5-3与pks5-4表现出对ABA的敏感表型。在外源ABA处理下,pks5-3与pks5-4种子萌发率降低,幼苗生长矮小、黄化。体外磷酸化测试显示,PKS5特异磷酸化ABA响应元件ABI5(ABA-insensitive 5)N末端多肽(1~211 aa)。q RT-PCR分析表明pks5-3与pks5-4突变体中ABI5下游ABA响应基因RAB18(RESPONSIVE TO ABA18)与EM6(LATE EMBRYOGENESIS ABUNDANT 6)表达均发生改变。这些研究结果表明,拟南芥PKS5通过磷酸化ABI5的N末端参与植物ABA响应过程。

  16. KnowIedge Teaching:An Effective Way to Promote the DeveIoPment of Thinking AbiIity%知识教学促进思维能力发展的有效方式



    KnowIedge and abiIity are restricted and Promoted reciProcaIIy. KnowIedge teaching may infIuence the deveIoPment of the abiIity in four ways of Positive and negative of baIance and imbaIance. KnowIedge can scarceIy naturaIIy changes into abiIity. Conscious Iearning and teaching are effective ways to Promote the deveIoPment of the abiIity to baIance and imbaIance. Thinking abiIity is the core comPetence of understanding and of PracticaI abiIity;knowIedge teaching,first of aII,can Promote the deveIoPment of thinking abiIity;we shouId choose effective teaching methods to Promote the Positive baIance and imbaIance deveIoPment of thinking abiIity,IogicaI thinking abiIity,thinking in images and imagination,diaIecticaI thinking and converse thinking,thinking of induction,and,deduction and reasoning.%知识与能力相互制约和促进。知识教学对能力发展的影响可能产生正负向平衡和不平衡的四种状态。知识自然而然地转化为能力的程度很有限,自觉的求知和教学方式是知识促进能力发展达到正向平衡和不平衡的有效方式。思维能力是认识和实践能力的核心能力,知识教学促进能力的发展首先是促进思维能力的发展,应选择有效教学方式促进探究思维能力、逻辑思维能力、形象思维和想象力、辩证思维和逆向思维能力、思维的归纳、演绎和推理能力实现正向平衡和不平衡的发展。

  17. Bacterial and abiotic decay in waterlogged archaeological Picea abies (L.) Karst studied by confocal Raman imaging and ATR-FTIR spectroscopy

    Pedersen, Nanna Bjerregaard; Gierlinger, Notburga; Thygesen, Lisbeth Garbrecht


    contained regions with intensities lower than sound S2 layers up to intensity values as high as the compound middle lamella (CML). CRI revealed strong depletion of carbohydrates in RM which indicated that EB are able to utilise the carbohydrate fraction of the cell wall effectively. Raman bands assigned......Waterlogged archaeological Norway spruce [Picea abies (L.) Karst] poles were studied by means of confocal Raman imaging (CRI) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) analysis to determine lignin and polysaccharide composition and distribution in the cell...

  18. 美国ABI Research预测:2010年全球至少有12家运营商提供LTE服务


    2010年提供LTE(Long Term Evoludon)网络及ITE月最务的通信运营商将至少有12家。美国ABI Research日前发布了上述预测。另外,预计2010年以前,使用通信速度不亚于使用连接线和DSL通信的高速数据服务的全球用户将达到近3400万人。

  19. Ecologie et croissance de Abies alba Mill. en peuplements purs et mélangés dans le Massif Vosgien (Nord-est de la France)

    Pinto, Paulina


    The objective of this work was to determine the ecological niche and growth of silver fir (Abies alba Mill.) in the Vosges Mountains (north-east France), in relation to environmental conditions and inter-specific competition. The data set used to study the ecological niche consisted in 325 phytoecological relevés extracted from the EcoPlant database. To study the growth of silver fir, 143 plots were carried out in this work using a stratified sampling design according to stand composition, el...

  20. Factores bióticos y abióticos que condicionan la biorremediación por Pseudomonas en suelos contaminados por hidrocarburos

    Gómez Romero, Sara Esmeralda; Gutiérrez Bustos, Diana Carolina; Hernández Marín, Ana María; Hernández Rodríguez, Cindy Zulay; Losada Casallas, Mariana; Mantilla Vargas, Paula Cecilia


    El proceso de biorremediación de hidrocarburos, contaminantes del suelo, llevado a cabo por Pseudomonas sp. está condicionado por factores bióticos y abióticos como los nutrientes, respiración, pH, humedad y temperatura propios del microorganismo, los cuales al ser modificados limitan o favorecen la capacidad metabólica de la bacteria y su adaptabilidad con el cambio de posición de los ácidos grasos de la membrana celular. La biorremediación de suelos ha demostrado ser una tecnología eficaz p...

  1. Research and Realization of Abis Interface Monitor Technology in User-Aware Intelligence Analysis System%用户感知智能分析系统Abis接口信令监测的研究

    李娟; 雒江涛


    以用户感知智能分析系统Abis接口信令监测功能为研究背景,分析了Abis接口协议栈结构,利用模块化思想实现了对Abis接口的监测.针对目前Abis接口CDR合成算法的不足,在进行CDR合成之前,创新性地采用了预处理技术来获取合成CDR时的散列Key,并利用一种新的递归回溯消息关联方法合成CDR.经现网实际测试,该方案已成功通过了Abis接口信令监测系统的测试和验证,效果良好.%Based on the user-aware intelligence analysis system Abis interface signaling monitoring, this paper analyzes the Abis interface protocal stack structure, and realizes to monitor the Abis interface, using of modular thinking. For the current lack of Abis interface CDR synthesis algorithm, this paper uses pretreatment technology to obtain the synthetic CDR Hash key before making CDR synthesis, and a new recursive backtrack message associated with synthesis of the CDR. This scheme has successfully passed the Abis interface signaling monitoring system test and has good results.

  2. [Response of Picea purpurea and Abies faxoniana tree rings at different slope aspects to rapid warming in western Sichuan, China].

    Guo, Bin-de; Zhang, Yuan-dong; Wang, Xiao-chun


    By using an empirical 'signal-free' standardization approach, we constructed four Picea purpurea and Abies faxoniana tree-ring chronologies at southeast and northwest slope aspects of Erdaohai and east slope aspect of Zharisi, Songpan, west Sichuan, China. The response analysis and multivariate analysis of variance between tree rings and climatic variables were conducted to explore the divergent responses of tree growth at different slope aspects to the recent warming climate. Results showed that tree growth of P. purpurea at east slope aspect was obviously accelerated (0.011 a-1) since rapid warming in 1980, whereas those at northwest slope aspect was significantly reduced (-0.006 a-1). Tree growth of P. purpurea at southeast slope aspect and A. faxoniana at northwest slope aspect decreased in significantly. With the rapid warming, growth-climate relationships of P. purpurea and A. faxoniana at different slope aspects changed significantly. After rapid warming in 1980, the promoting effects of growing season temperature (GST) on P. purpurea growth at east slope increased significantly, while the inhibitory effects of GST on its growth at southeast and northwest slopes also increased significantly. However, the effects of GST on A. faxoniana growth at northwest slope did not change significantly before and after rapid warming. The effects of precipitation in May (PM) on P. purpurea growth at east slope was changed from inhibition before rapid warming to significant promotion after rapid warming, while the inhibitory effects of PM on P. purpurea growth at southeast and northwest slopes increased significantly. For A. faioniana at northwest slope, however, it did not change obviously before and after rapid warming. The response analysis between tree growth and the Palmer drought severity index (PDSI) showed that soil moisture variations at different slope aspects were an important reason of tree-ring growth response difference since rapid warming. In addition, the

  3. Tree growth and competition in an old-growth Picea abies forest of boreal Sweden: influence of tree spatial patterning

    Fraver, Shawn; D'Amato, Anthony W.; Bradford, John B.; Jonsson, Bengt Gunnar; Jönsson, Mari; Esseen, Per-Anders


    Question: What factors best characterize tree competitive environments in this structurally diverse old-growth forest, and do these factors vary spatially within and among stands? Location: Old-growth Picea abies forest of boreal Sweden. Methods: Using long-term, mapped permanent plot data augmented with dendrochronological analyses, we evaluated the effect of neighbourhood competition on focal tree growth by means of standard competition indices, each modified to include various metrics of trees size, neighbour mortality weighting (for neighbours that died during the inventory period), and within-neighbourhood tree clustering. Candidate models were evaluated using mixed-model linear regression analyses, with mean basal area increment as the response variable. We then analysed stand-level spatial patterns of competition indices and growth rates (via kriging) to determine if the relationship between these patterns could further elucidate factors influencing tree growth. Results: Inter-tree competition clearly affected growth rates, with crown volume being the size metric most strongly influencing the neighbourhood competitive environment. Including neighbour tree mortality weightings in models only slightly improved descriptions of competitive interactions. Although the within-neighbourhood clustering index did not improve model predictions, competition intensity was influenced by the underlying stand-level tree spatial arrangement: stand-level clustering locally intensified competition and reduced tree growth, whereas in the absence of such clustering, inter-tree competition played a lesser role in constraining tree growth. Conclusions: Our findings demonstrate that competition continues to influence forest processes and structures in an old-growth system that has not experienced major disturbances for at least two centuries. The finding that the underlying tree spatial pattern influenced the competitive environment suggests caution in interpreting traditional tree

  4. Population dynamics and genetic changes of Picea abies in the South Carpathians revealed by pollen and ancient DNA analyses

    Braun Mihály


    Full Text Available Abstract Background Studies on allele length polymorphism designate several glacial refugia for Norway spruce (Picea abies in the South Carpathian Mountains, but infer only limited expansion from these refugia after the last glaciation. To better understand the genetic dynamics of a South Carpathian spruce lineage, we compared ancient DNA from 10,700 and 11,000-year-old spruce pollen and macrofossils retrieved from Holocene lake sediment in the Retezat Mountains with DNA extracted from extant material from the same site. We used eight primer pairs that amplified short and variable regions of the spruce cpDNA. In addition, from the same lake sediment we obtained a 15,000-years-long pollen accumulation rate (PAR record for spruce that helped us to infer changes in population size at this site. Results We obtained successful amplifications for Norway spruce from 17 out of 462 pollen grains tested, while the macrofossil material provided 22 DNA sequences. Two fossil sequences were found to be unique to the ancient material. Population genetic statistics showed higher genetic diversity in the ancient individuals compared to the extant ones. Similarly, statistically significant Ks and Kst values showed a considerable level of differentiation between extant and ancient populations at the same loci. Lateglacial and Holocene PAR values suggested that population size of the ancient population was small, in the range of 1/10 or 1/5 of the extant population. PAR analysis also detected two periods of rapid population growths (from ca. 11,100 and 3900 calibrated years before present (cal yr BP and three bottlenecks (around 9180, 7200 and 2200 cal yr BP, likely triggered by climatic change and human impact. Conclusion Our results suggest that the paternal lineages observed today in the Retezat Mountains persisted at this site at least since the early Holocene. Combination of the results from the genetic and the PAR analyses furthermore suggests that the higher

  5. Raman Spectroscopic Online Investigation of Respiratory Quotients in Pinus Sylvestris and Picea Abies during Drought and Shading

    Hanf, S.; Fischer, S.; Hartmann, H.; Trumbore, S.; Popp, J.; Frosch, T.


    Drought and heat waves have been linked to forest mortality event across the globe. The underlying physiological processes are still not elucidated but both tree carbon and water relations have been identified as the driving forces. While studies on tree hydraulics are straightforward, studies on the tree carbon balance are not. For example, the use of different carbon compounds for maintenance respiration during drought cannot be assessed with measurements of carbon pools but requires real-time analyses of respiration stoichiometry. However, so far there were no technical solutions for such applications. Here we introduce cavity-enhanced Raman spectrometry (CERS) for simultaneous real-time monitoring of O2 and CO2 and rapid and continuous quantification of dark respiration rates and the respiratory quotient (RQ), i.e. the ratio of CO2 produced over O2 consumed during respiration. This ratio indicates the proportions of different substrates (carbohydrates [COH], lipids, proteins) used during respiration and allows fundamental insights into tree physiology. CERS combines high temporal resolution with a high dynamic concentration range for all important gases, ranging from few ppm to 100 vol. % with a single measurement every few seconds. The respiration analysis of tree branches was performed in a closed chamber for two species of different drought tolerance, Pinus sylvestris and Picea abies. We applied not only drought but also a shading treatment because both cause reductions in carbon assimilation rates but have different effects on tree hydraulics. Declines in RQ during shading in both species indicate a switch from pure COH metabolism to a mixture of COH, lipids and proteins. During drought such declines occurred only in the drought-tolerant pine but not in spruce and the underlying more dynamic carbon use strategy in pine may provide a physiological basis for its drought tolerance, more detailed investigation still pending. Our study highlights the suitability

  6. General practitioner advice on physical activity: Analyses in a cohort of older primary health care patients (getABI

    Thiem Ulrich


    Full Text Available Abstract Background Although the benefits of physical activity for health and functioning are recognized to extend throughout life, the physical activity level of most older people is insufficient with respect to current guidelines. The primary health care setting may offer an opportunity to influence and to support older people to become physically active on a regular basis. Currently, there is a lack of data concerning general practitioner (GP advice on physical activity in Germany. Therefore, the aim of this study was to evaluate the rate and characteristics of older patients receiving advice on physical activity from their GP. Methods This is a cross-sectional study using data collected at 7 years of follow-up of a prospective cohort study (German epidemiological trial on ankle brachial index, getABI. 6,880 unselected patients aged 65 years and above in the primary health care setting in Germany were followed up since October 2001. During the 7-year follow-up telephone interview, 1,937 patients were asked whether their GP had advised them to get regular physical activity within the preceding 12 months. The interview also included questions on socio-demographic and lifestyle variables, medical conditions, and physical activity. Logistic regression analysis (unadjusted and adjusted for all covariables was used to examine factors associated with receiving advice. Analyses comprised only complete cases with regard to the analysed variables. Results are expressed as odds ratios (ORs with 95% confidence intervals (95% CI. Results Of the 1,627 analysed patients (median age 77; range 72-93 years; 52.5% women, 534 (32.8% stated that they had been advised to get regular physical activity. In the adjusted model, those more likely to receive GP advice on physical activity were men (OR [95% CI] 1.34 [1.06-1.70], patients suffering from pain (1.43 [1.13-1.81], coronary heart disease and/or myocardial infarction (1.56 [1.21-2.01], diabetes mellitus (1

  7. Modeling effects of temperature and precipitation on carbon characteristics and GHGs emissions in Abies fabric forest of subalpine

    LU Xuyang; CHENG Genwei; XIAO Feipeng; FAN Jihui


    Abies fabric forest in the eastern slope of Gongga mountain is one type of subalpine dark coniferous forests of southwestern China.It is located on the southeastern edge of the Qinghai-Tibet plateau and is sensitive to climatic changes,A process-oriented biogeochemical model,Forest-DNDC,was applied to simulate the effects of climatic factors,temperature and precipitation changes on carbon characteristics,and greenhouse gases (GHGs) emissions inA.fabric forest.Validation indicated that the Forest-DNDC could be used to predict carbon characteristics and GHGs emissions with reasonable accuracy.The model simulated carbon fluxes,soil carbon dynamics,soil CO2,N2O,and NO emissions with the changes of temperature and precipitation conditions.The results showed that with variation in the baseline temperature from-2℃ to+2℃,the gross primary production (GPP) and soil organic carbon (SOC)increased,and the net primary production (NPP) and net ecosystem production (NEP) decreased because of higher respiration rate.With increasing baseline precipitation the GPP and NPP increased slightly,and the NEP and SOC showed decreasing trend.Soil CO2 emissions increased with the increase of temperature,and CO2 emissions changed little with increased baseline precipitation.With increased temperature and decreased baseline temperature,the total annual soil N2O emissions increased.With the variation of baseline temperature from -2℃ to +2℃,the total annual soil NO emissions increased.The total annual N2O and NO emissions showed increasing trends with the increase of precipitation.The biogeochemical simulation of the typical forest indicated that temperature changes strongly affected carbon fluxes,soil carbon dynamics,and soil GHGs emissions.The precipitation was not a principal factor affecting carbon fluxes,soil carbon dynamics,and soil CO2 emissions,but changes in precipitation could exert strong effect on soil N2O and NO emissions.

  8. Ibn Abi Ussaibea "Oyoun aI-Anbaa Fi Tabaqat al-Attiba" a selected review of this Index of Physicians and their Works; (from early Greeks to his time in Egypt).

    Kotby, M Nasser; Wahba, Hassan; Mabrouk, Amr


    Muwaffaq-al-Deen abu-al-Abbas Ahmad ibn Abi Ussaibea (1203-1270) was born in Damascus. He started his brilliant career in his birth place then moved to Cairo where he worked and excelled for the rest of his life. His learning was intensified by the scholarly contacts of the intellectual leaders of the day. In this study we review The Index of Physicians-Oyoun al-Anbaa Fi Tabaqat al-Attiba--(Sources of Information in the Classes of Physicians) of ibn Abi Ussaibea.The biographies in this book do not just cover physicians only but also the learned people of his day whose knowledge and expertise covered medicine, astronomy, mathematics, philosophy, and botany. For this reason the book of ibn Abi Ussaibea represents an indispensable source of the scientific and philosophical achievements of the Arabic/Islamic civilization.

  9. Niveles y Patrones de variación química en resinas de las hojas del género Abies del norte de México

    Yolanda Nava Cruz; Francisco J. Espinosa García; Glenn R. Furnier Whitelaw


    El género Abies se caracteriza por dificultades en su taxonomía, pues la hibridación interespecífica es frecuente y presenta alta variación morfológica intraespecífica. Así, las propuestas para clasificar a las especies de Abies son discordantes. En este estudio se analizó por cromatografía de gases la composición de la fracción volátil de las resinas foliares de 11 poblaciones de cuatro especies de Abies del norte de México (dos A. concolor, tres A. durangensis, dos A. coahuilensis y cuatro ...

  10. Constitutive Equations and ANN Approach to Predict the Flow Stress of Ti-6Al-4V Alloy Based on ABI Tests

    Wang, Fuzeng; Zhao, Jun; Zhu, Ningbo


    The flow behavior of Ti-6Al-4V alloy was studied by automated ball indentation (ABI) tests in a wide range of temperatures (293, 493, 693, and 873 K) and strain rates (10-6, 10-5, and 10-4 s-1). Based on the experimental true stress-plastic strain data derived from the ABI tests, the Johnson-Cook (JC), Khan-Huang-Liang (KHL) and modified Zerilli-Armstrong (ZA) constitutive models, as well as artificial neural network (ANN) methods, were employed to predict the flow behavior of Ti-6Al-4V. A comparative study was made on the reliability of the four models, and their predictability was evaluated in terms of correlation coefficient (R) and mean absolute percentage error. It is found that the flow stresses of Ti-6Al-4V alloy are more sensitive to temperature than strain rate under current experimental conditions. The predicted flow stresses obtained from JC model and KHL model show much better agreement with the experimental results than modified ZA model. Moreover, the ANN model is much more efficient and shows a higher accuracy in predicting the flow behavior of Ti-6Al-4V alloy than the constitutive equations.

  11. Relación entre variabilidad biogeoquímica y rasgos estructurales de la comunidad microbiana en suelos forestales: Bosques de Abies pinsapo como caso de estudio

    P. Torres-Cañabate


    Full Text Available Relación entre variabilidad biogeoquímica y rasgos estructurales de la comunidad microbiana en suelos forestales: Bosques de Abies pinsapo como caso de estudio. Tradicionalmente, los estudios biogeoquímicos consideraban el suelo como una "caja negra", conectada con el resto del ecosistema mediante flujos de entrada y de salida de nutrientes, en la que éstos experimentan transformaciones mediadas engran parte por microorganismos desconocidos. El desarrollo de nuevas técnicas moleculares ha incrementado el conocimiento sobre lascomunidades microbianas del suelo. Estudios recientes han puesto de manifiesto correspondencias entre la variabilidad de la disponibilidad de nutrientes y la estructura de la comunidad microbiana en suelos forestales. A este respecto, hemos empleado bosques de Abies pinsapo como modelo ya que muestran una gran variabilidad biogeoquímica asociada a diferencias litológicas y sucesionales, mientras que la estacionalidad mediterránea provoca una alta variabilidad intra-anual en la disponibilidad de nutrientes. Además, existen pinsapares sujetos alimitación o a saturación de nitrógeno. Mediante análisis del polimorfismo de longitud de fragmentos de restricción terminal (T-RFLP, hemos puesto de manifiesto que los patrones de variabilidad biogeoquímica descritos en los pinsapares se corresponden con cambios en la composición y estructura de sus comunidades bacterianas.

  12. Comparison of the ABI 7700 System (TaqMan) and Competitive PCR for Quantification of IS6110 DNA in Sputum during Treatment of Tuberculosis

    Desjardin, L. e.; Chen, Y.; Perkins, M. D.; Teixeira, L.; Cave, M. D.; Eisenach, K. D.


    Mycobacterium tuberculosis can persist in sputum for long periods of time after the initiation of antituberculosis chemotherapy. The purpose of this study was to determine whether quantitative estimates of M. tuberculosis DNA in sputum correlate with the numbers of viable bacilli and thus measure the therapeutic response of patients during treatment. Two methods of M. tuberculosis DNA quantification were examined by using DNA isolated from sputum specimens serially collected during the course of chemotherapy. A competitive PCR assay was compared to an automated system of real-time quantification with the ABI Prism 7700 Sequence Detection System (TaqMan). The ABI 7700 system uses standard PCR in conjunction with a fluorogenic probe in which the intensity of fluorescence is proportional to the amount of target DNA present. The results showed that both PCR systems are reproducible and accurate. The amounts of M. tuberculosis DNA quantified in sputum corresponded well with the numbers of acid-fast bacilli (AFB) counted by microscopy. Before initiation of antituberculosis therapy, measures of AFB, M. tuberculosis DNA, and cultivable bacilli were similar, suggesting that quantification of DNA is a good method for measuring the initial bacillary load. However, the rate of disappearance of both AFB and M. tuberculosis DNA did not correlate with the decline in cultivable bacilli in the specimen; therefore, these tests are not appropriate for monitoring treatment efficacy. PMID:9650945

  13. ABI、PWV与冠心病危险因素关系的研究%Study the Relation of ABI, PWV and Coronary Heart Disease Risk Factors

    杨志; 万艳芳; 张军; 王长厚; 杨静; 牛和平; 马晓丽


    Objective:To Evaluated the relationship of ABI,PWV and coronary heart disease risk factors.Methods:Retrospectively analyzed ABI,PWV and related risk factors of coronary heart disease had Coronary angiography in our hospital.Results:There were 506 cases with coronary heart disease in 657 cases confirmed by coronary angiography.Smoking or who smoke more than 5 years,hypertension,diabetes,abnormal lipid metabolism in coronary heart disease patiets was significantly higher than in non-coronary heart disease patients (P<0.01);Obesity in coronary heart disease higher than non-coronary heart disease (P<0.05);the ABI values were significantly downward trend,PWV values were significantly Upward trend in those with smoking,hypertension,diabetes,abnormal lipid metabolism,obesity (P<0.05).Severe coronary artery disease (more than double vessel disease and stenosis≥70%)was 186 cases,the ABI,PWV value in Severe coronary artery disease were significant Statistically differences with non-coronary heart disease(P<0.01).Conclusions:Smoking,hypertension,diabetes,dyslipidemia,Obesity may affect the development of coronary heart disease,and the ABI,PWV can expressed comprehensive impact ]for coronary artery that cause by the variety of risk factors.%目的:综合评价ABI、PWV与冠心病危险因素的关系.方法:对我院因冠心病住院并行冠状动脉造影的心脏病患者的ABI、PWV及相关危险因素进行回顾性分析.结果:经冠脉造影证实657例患者中冠心病患者为506例;吸烟或曾经吸烟5年以上者、高血压、糖尿病、脂代谢异常冠心病患者明显高于非冠心病患者(P<0.01);肥胖冠心病患者高于非冠心病患者(P<0.05);有吸烟、高血压、糖尿病、脂质代谢异常、肥胖患者的ABI数值有明显下降趋势,PWV数值有明显增高趋势(P<0.05);严重冠心病患者(双支以上病变且血管狭窄≥70%)共186例,其ABI、PWV值与非冠心病患者差异有显著统计学意义(P<0

  14. Evaluating the species- and site-specific differences in the physiological response of Picea abies, Fagus sylvatica and Larix decidua to drought

    Hartl-Meier, Claudia; Rothe, Andreas; Treydte, Kerstin


    Sensitive regions like the Alps are very vulnerable to climate change. Especially warmer temperatures and a higher frequency of drought periods may imply strong effects on mountain ecosystems. In the Northern Limestone Alps, temperatures were already 1 °C higher (compared to the reference period 1941-1970) in the last two decades. Within a Bavarian-Austrian EU-project (INTERREG program) we investigated long-term growth patterns of mountain tree species and a possible growth effect caused by climate change using a dendroecological approach. In total we measured the ring widths of ~1300 living, on average 180 year old trees. The samples were taken along altitudinal gradients, ranging from 500 up to 1700 m a.s.l., in five different regions in the Northern Austrian and Bavarian Limestone Alps, covering the most prevalent coniferous (Picea abies, Abies alba, Larix decidua, Pinus sylvestris) and broad-leafed (Fagus sylvatica, Acer pseudoplatanus) mountain forest species. To get more detailed information about the physiological response to climate and especially drought events of different tree species, an additional study was conducted in the Kalkalpen Nationalpark, Austria. Stable isotopes (δ13C and δ18O) of Picea abies, Fagus sylvatica and Larix decidua tree-rings (8 trees per species and site) were analysed at three different sites. The sites are located at the montane elevation level (900 m a.s.l.) on a south-facing and a north-facing slope as well on a plateau situation with deeper soils. Our main focus deals with the following questions: i) Is it possible to identify "drought events" in a region like the Alps with generally humid precipitation conditions (1400 mm/a), by analysing stable isotopes in tree rings? ii) Are there species- and/or site-specific differences in the isotopic signatures - also with respect to the trees' climate response? We will present (i) the isotopic signatures for the common period 1970-2010, (ii) their response to climate conditions

  15. The mycorrhizal fungus Amanita muscaria induces chitinase activity in roots and in suspension-cultured cells of its host Picea abies.

    Sauter, M; Hager, A


    A cell-wall fraction of the mycorrhizal fungus Amanita muscaria increased the chitinase activity in suspension-cultured cells of spruce (Picea abies (L.) Karst.) which is a frequent host of Amanita muscaria in nature. Chitinase activity was also increased in roots of spruce trees upon incubation with the fungal elicitor. Non-induced levels of chitinase activity in spruce were higher in suspension cells than in roots whereas the elicitorinduced increase of chitinase activity was higher in roots. Treatment of cells with hormones (auxins and cytokinin) resulted in a severalfold depression of enzyme activity. However, the chitinase activity of hormone-treated as well as hormone-free cells showed an elicitor-induced increase. Suspension cells of spruce secreted a large amount of enzyme into the medium. It is postulated that chitinases released from the host cells in an ectomycorrhizal system partly degrade the fungal cell walls, thus possibly facilitating the exchange of metabolites between the symbionts.

  16. Ibn Abi Al-Hadid’s and Ibn Meysam’s interpretations comparative analysis for the purpose of Nahj Al-Balagha 1 to 34 sermons lexicological understanding

    Shahnaz Azizi


    Full Text Available Nahj Al-Balagha, as a valuable literary, scientific and Palladian work is the focus of scientists in various Islamic fields such as literature, history, ethics, theosophy, philosophy and discourse, politics and government and management. Now, we are the inheritors of various massive explications on Nahj Al-Balagha which is approached by many from various perspectives. The lexicological terms proposition and analysis, before going to the interpretations, is a general method of interpreting a text, but not all of the explicators have acted in one single level of elaboration and analysis of the terminologies. The study of the wording can be based on the lexical, literary, eloquence, discourse, philosophical and theosophical methods. In the present study the comparative analysis and the lexical exegesis and the interpretations by the great expositor of Nahj Al-Balagha, Ibn Abi Al-Hadid and Ibn Meysam, is presented on the first “34 sermons of Nahj Al-Balagha”.

  17. Effects of Steaming, Drying Temperature, and Adhesive Type on Static Bending Properties of LVL made of Picea orientalis and Abies nordmanniana veneers

    Huseyin Peker


    Full Text Available The modulus of elasticity (MOE and modulus of rupture (MOR were evaluated for laminated veneer lumber (LVL in static bending. The studied species were spruce (Picea orientalis and fir (Abies nordmanniana originated from the Eastern Black sea region and prepared with phenol-formaldehyde and melamine-urea-formaldehyde. The effect of wood species, steaming, drying temperature, and type of adhesive on static bending MOE and MOR were determined. According to the experimental results, the bending strength of spruce wood (Maçka treated with phenol formadehyde adhesives is the highest for the specimens steamed for 6 h at a drying temperature of 110 ºC. Furthermore, the modulus of elasticity for spruce wood (Maçka treated with phenol formadehyde adhesive is the highest for the specimens steamed for 12 h and subjected to a drying temperature of 150 ºC.

  18. Effects of deicing salt on the vitality and health of two spruce species, Picea abies Karst., and Picea glehnii Masters planted along roadsides in northern Japan.

    Kayama, M; Quoreshi, A M; Kitaoka, S; Kitahashi, Y; Sakamoto, Y; Maruyama, Y; Kitao, M; Koike, T


    In northern Japan, the growth of Picea abies Karst., and Picea glehnii Masters, which have been planted along the highways, is often suppressed due to several environmental stresses. To examine the adverse effects of deicing salt, the primary source of stress,we measured needle life span, photosynthetic capacity, and water potential and transpiration rate of the two spruce species at a site with damaged trees, near the roadside and a site with healthy trees, located far from the highway. Results from the analysis showed large amounts of sodium and chlorine in the soil and snow at the damaged site. These elements had accumulated in the needles of the spruce. Moreover, physiological traits of the spruce, at the damaged site were also affected. Therefore, we concluded that poor physiological traits might be attributed to an accumulation of deicing salt in the needles, resulting in the suppression of tree growth.

  19. Holophyllane A: A Triterpenoid Possessing an Unprecedented B-nor-3,4-seco-17,14-friedo-lanostane Architecture from Abies holophylla

    Kim, Chung Sub; Oh, Joonseok; Subedi, Lalita; Kim, Sun Yeou; Choi, Sang Un; Lee, Kang Ro


    A novel triterpenoid, holophyllane A (1), featuring a B-nor-3,4-seco-17,14-friedo-lanostane, along with its putative precursor, compound 2 were isolated from the methanol extract of the trunks of Abies holophylla. The 2D structure and relative configuration of 1 were initially determined via analysis of 1D and 2D NMR spectroscopic data and the assignment was confirmed by quantum mechanics-based NMR chemical shift calculations. The absolute configuration was established by comparison of the experimental and simulated ECD data generated at different theory levels. Compounds 1 and 2 exhibited moderate to weak cytotoxicity and significant inhibitory activity against nitric oxide (NO) production. PMID:28252664

  20. Histological studies on the bundle sheath in needles of Picea abies (L. ) Karst. , diseased or fumigated with SO/sub 2/

    Maier-Maercker, U.


    The paper reports disorganisations within the bundle sheath in current year needles of diseased Picea abies. Similar symptoms were produced by fumigation of young trees for 20 days with 0.3 ppm SO/sub 2/. The lignified radial walls of the sheath cells were in a state of partial delignification and the lumina contained granular substances which absorbed UV light at 280 nm. While autofluorescence of the radial walls was weak in injured material the granular cell contents were heavily fluorescent. Studies of structural features and the outcome of feeding experiments using berberine sulfate and Tl/sub 2/SO/sub 4/ suggest that the bundle sheath is involved in the water transport from the vascular system towards the evaporating surfaces of the needle. The activity of wall bound peroxidase was localized histochemically; the possible role of this enzyme complex is discussed.

  1. Growth losses in Swiss forests caused by ozone: epidemiological data analysis of stem increment of Fagus sylvatica L. and Picea abies Karst.

    Braun, Sabine; Schindler, Christian; Rihm, Beat


    The estimate of growth losses by ozone exposure of forest trees is a significant part in current C sequestration calculations and will also be important in future modeling. It is therefore important to know if the relationship between ozone flux and growth reduction of young trees, used to derive a Critical Level for ozone, is also valid for mature trees. Epidemiological analysis of stem increment data from Fagus sylvatica L. and Picea abies Karst. observed in Swiss forest plots was used to test this hypothesis. The results confirm the validity of the flux-response relationship at least for beech and therefore enable estimating forest growth losses by ozone on a country-wide scale. For Switzerland, these estimates amount to 19.5% growth reduction for deciduous forests, 6.6% for coniferous forests and 11.0% for all forested areas based on annual ozone stomatal uptake during the time period 1991-2011.

  2. Functions for biomass and basic density of stem, crown and root system of Norway spruce (Picea abies (L.) Karst.) in Denmark

    Skovsgaard, Jens Peter; Bald, Caroline; Nord-Larsen, Thomas


    Models for predicting the biomass of forest trees are becoming increasingly important for assessing forest resources and carbon sequestration in forests. We developed functions for predicting the biomass and basic density of above- and below-ground parts of Norway spruce (Picea abies (L.) Karst...... variety of tree sizes, stand treatments and growth conditions. The models are believed to substantially improve national estimates of carbon sequestration and biomass resources........) in Denmark. Separate models were developed for branches (including foliage), stem and the below-ground stump and root system as well as for the aggregate components of total above-ground biomass and total tree biomass. Trees were sampled in 14 forest stands, reflecting the range of growth conditions...

  3. The Primary Diterpene Synthase Products of Picea abies Levopimaradiene/Abietadiene Synthase (PaLAS) Are Epimers of a Thermally Unstable Diterpenol*

    Keeling, Christopher I.; Madilao, Lina L.; Zerbe, Philipp; Dullat, Harpreet K.; Bohlmann, Jörg


    The levopimaradiene/abietadiene synthase from Norway spruce (Picea abies; PaLAS) has previously been reported to produce a mixture of four diterpene hydrocarbons when incubated with geranylgeranyl diphosphate as the substrate: levopimaradiene, abietadiene, neoabietadiene, and palustradiene. However, variability in the assay products observed by GC-MS of this and orthologous conifer diterpene synthases over the past 15 years suggested that these diterpenes may not be the initial enzyme assay products but are rather the products of dehydration of an unstable alcohol. We have identified epimers of the thermally unstable allylic tertiary alcohol 13-hydroxy-8(14)-abietene as the products of PaLAS. The identification of these compounds, not previously described in conifers, as the initial products of PaLAS has considerable implications for our understanding of the complexity of the biosynthetic pathway of the structurally diverse diterpene resin acids of conifer defense. PMID:21518766

  4. A vida póstuma de Aby Warburg: por que seu pensamento seduz os pesquisadores contemporâneos da imagem?

    Mauricio Lissovsky

    Full Text Available A fama de Aby Warburg (1866-1929 sempre superou o conhecimento de sua obra. Mas, nas últimas duas décadas, alguns dos mais importantes pensadores da estética - como Georges Didi-Huberman e Giorgio Agamben - encontram neste obscuro historiador da arte alemão um precursor de suas próprias investigações. Partindo do debate sobre o legado de Warburg nas obras de Ernst Gombrich e Erwin Panofsky, este ensaio procura historiar a trajetória deste resgate e repertoriar os conceitos e procedimentos warburgianos que mais mobilizam os teóricos contemporâneos. Sugere-se, por fim, que é devido à crescente influência de Walter Benjamin e às inquietações suscitadas pelo estatuto da imagem na contemporaneidade que os estudos de Warburg voltam a servir de referência para historiadores e estudiosos da imagem.

  5. Population differentiation in tree-ring growth response of white fir (Abies concolor) to climate: Implications for predicting forest responses to climate change

    Jensen, Deborah Bowne [Univ. of California, Berkeley, CA (United States)


    Forest succession models and correlative models have predicted 200--650 kilometer shifts in the geographic range of temperate forests and forest species as one response to global climate change. Few studies have investigated whether population differences may effect the response of forest species to climate change. This study examines differences in tree-ring growth, and in the phenotypic plasticity of tree-ring growth in 16-year old white fir, Abies concolor, from ten populations grown in four common gardens in the Sierra Nevada of California. For each population, tree-ring growth was modelled as a function of precipitation and degree-day sums. Tree-ring growth under three scenarios of doubled CO2 climates was estimated.

  6. Aby Warburg, Homo victor’ [A translation (back into English, and with some revisions of the article that appeared in French: ‘Aby Warburg, Homo victor’, in Cahiers du Musée national d’art moderne 118, 2011/12, 81-101

    Christopher S. Wood


    Full Text Available This essay reflects on the reception of Aby Warburg’s writings in the last several decades. A common theme of the recent literature is that Warburg was misunderstood or misread by the scholars who looked after the library and the legacy in London, in particular Ernst H. Gombrich. Here it is proposed that Gombrich, with his mistrust of traditional aesthetics, his affirmative humanism, and his hopes for a convergence of humanistic studies with the natural sciences, grasped aspects of Warburg’s thought that much of the recent literature has missed.

  7. Simulating Carbon Sequestration and GHGs Emissions in Abies fabric Forest on the Gongga Mountains Using a Biogeochemical Process Model Forest-DNDC

    LU Xuyang; CHENG Genwei; XIAO Feipeng; HUO Changfu


    The process-oriented model Forest-DNDC describing biogeochemical cycling of C and N and GHGs (greenhouse gases) fluxes (CO2, NO and N2O) in forest ecosystems was applied to simulate carbon sequestration and GHGs emissions in Abies fabric forest of the Gongga Mountains at southeastern edge of the Tibetan Plateau. The results indicated that the simulated gross primary production (GPP) of Abies fabric forest was strongly affected by temperature. The annual total GPP was 24,245.3 kg C ha-1 yr-1 for 2005 and 26,318.8 kg C ha-1 yr-1 for 2006, respectively. The annual total net primary production (NPP) was 5,935.5 and 4,882.2 kg C ha-1 yr-1 for 2005 and 2006, and the annual total net ecosystem production (NEP) was 4,815.4 and 3,512.8 kg C ha-1 yr-1 for 2005 and 2006, respectively. The simulated seasonal variation in CO2 emissions generally followed the seasonal variations in temperature and precipitation. The annual total CO2 emissions were 3,109.0 and 4,821.0 kg C ha-1 yr-1 for 2005 and 2006, the simulated annual total N2O emissions from forest soil were 1.47 and 1.36 kg N ha-1 yr-1 for 2005 and 2006, and the annual total NO emissions were 0.09 and 0.12 kg N ha-1 yr-1 for 2005 and 2006, respectively.

  8. The effects of land use change on carbon content in the aerial biomass of an Abies religiosa (Kunth Schltdl. et Cham. forest in central Veracruz, Mexico

    M.R. Pineda Lopez


    Full Text Available Aim of study. Were analyzed patterns and dynamics of forest cover change and land use for the period 1995-2005, and estimated carbon content in biomass in a forest of fir (Abies religiosa.Area of study. Ejido El Conejo in the Cofre de Perote National Park, Veracruz, Mexico.Material and Methods. Spatial data (maps, orthophotographs were used: (UTM; GRS 80 spheroid; zone 14, datum ITRF92; units: metres. Maps for 1995 and 2005 were constructed and compared. Carbon content in the aboveground biomass of fir (Abies religiosa was estimated using an equation specific to species.Main results. After one decade, the forested area had increased slightly (0.25%. The main type of land cover change was from shrubland to forest (probability of change 0.41; the change from forestland to shrubland with trees was 0.05%, and from forest to agriculture was 0.02%. Data from 2008 confirmed that the A. religiosa forest located in the ejido is distributed in largely unconnected fragments, of which some have a high density of young trees. Estimated carbon content in the aboveground biomass (163.51 Mg ha-1 was higher than that estimated for other forests of the same species in Mexico.Research highlights. It is recommended that ecological corridors be established throughout the reforested area, in order to connect these fragments (and increase carbon sequestration and favour faunal and floral conservation. Finally, recognition should be given to the high vulnerability of A. religiosa forests to climate change, given their high degree of fragmentation and critical altitudinal limit.Key words: Aboveground biomass; carbon storage; ecological corridors; fir forest.

  9. The effects of land use change on carbon content in the aerial biomass of an Abies religiosa (Kunth Schltdl. et Cham.) forest in central Veracruz, Mexico

    Pineda-Lopez, M. R.; Sanchez-Velasquez, L. R.; Vazquez-Dominguez, G.; Rojo-Alboreca, A.


    Aim of study.) Were analyzed patterns and dynamics of forest cover change and land use for the period 1995-2005, and estimated carbon content in biomass in a forest of fir (Abies religiosa). Area of study. Ejido El Conejo in the Cofre de Perote National Park, Veracruz, Mexico. Material and Methods. Spatial data (maps, ortho photographs) were used: (UTM); GRS 80 spheroid; zone 14, datum ITRF92; units: metres. Maps for 1995 and 2005 were constructed and compared. Carbon content in the aboveground biomass of fir (Abies religiosa) was estimated using an equation specific to species. Main results. After one decade, the forested area had increased slightly (0.25%). The main type of land cover change was from shrubland to forest (probability of change 0.41); the change from forestland to shrubland with trees was 0.05%, and from forest to agriculture was 0.02%. Data from 2008 confirmed that the A. religiosa forest located in the ejido is distributed in largely unconnected fragments, of which some have a high density of young trees. Estimated carbon content in the aboveground biomass (163.51 Mg ha-1) was higher than that estimated for other forests of the same species in Mexico. Research highlights. It is recommended that ecological corridors be established throughout the reforested area, in order to connect these fragments (and increase carbon sequestration) and favour faunal and floral conservation. Finally, recognition should be given to the high vulnerability of A. religiosa forests to climate change, given their high degree of fragmentation and critical altitudinal limit. (Author) 55 refs.

  10. Establecimiento de una red de equilibrios biológicos en ecosistemas con presencia de pinsapo (Abies pinsapo Boiss. en Andalucía.

    Navarro Cerrillo, R. M.


    Full Text Available In 2001, the Consejería de Medio Ambiente of the Junta de Andalucía established a Monitoring Network on ecosystems of Abies pinsapo Boiss.for evaluating the phytosanitary state in the natural range areas of A. pinsapo in the Iberian Península: Sierra de las Nieves (Málaga, Spain, Sierra de Grazalema (Cadiz, Spain and Los Reales de Sierra Bermeja (Málaga, Spain. This network is based on a 1 x 1 Km grid established throughout the forest stands where Abies pinsapo is present, given by the Mapa Forestal de España (Spanish land cover map, RUIZ DE LA TORRE, 1990. The Network has been constructed from 35 sample plots, which will be visited annually (in summer in order to assess the state of trees. This article describes the network design process and the main results from the first campaign of sampling.

    En mai 2001, la Consejería de Medio Ambiente-Junta de Andalucía a mis sur pied un Réseau pour la surveillance systématique et multitemporelle de l'état de santé et vitalité des forets de Abies pinsapo Boiss. dans ses aires de répartition naturelle dans la Péninsule Ibérique: Sierra de las Nieves (Málaga, Espagne, Sierra de Grazalema (Cádiz, Espagne et Los Reales de Sierra Bermeja (Málaga, Espagne. Cet inventaire a été réalisé à partir d'un réseau d'échantillonnage systématique selon un maillage de 1 x 1 kilomètres couvrant la surface des forêts avec présence de Abies pinsapo, d'après la Mapa Forestal de España (Carte Forestière Espagnole, RUIZ DE LA TORRE, 1990. Après Rétablissement, l'inventaire a été constitué par 35 parcelles d'observation, qui seront visitées annuellement (en été pour évaluer l'état des arbres. Cette note présente le dessin de ce réseau et les résultats de la première campagne de terrain.
    En la primavera del año 2001, la Consejería de Medio Ambiente de la Junta de Andalucía estableció una Red de Equilibrios Biol

  11. Composition and structure of varves in Lake Żabińskie, northeastern Poland: a potential for high-resolution reconstruction of meteorological conditions

    Tylmann, Wojciech; Bonk, Alicja; Amann, Benjamin; Butz, Christoph; Enters, Dirk; Kinder, Małgorzata; Grosjean, Martin


    Reconstructions of paleoclimatic and paleoenvironmental data from sediment records require a detailed knowledge of the physical, chemical and biological factors which influence sediment-formation processes and signal preservation in lake sediments. This can be achieved by process studies and high-resolution analysis of sediments that provide an opportunity to calibrate varves and paleolimnological proxies against instrumental hydrological and meteorological data. Here we present results from Lake Żabińskie located in northeastern Poland with the aim to understand the relationship between meteorological conditions, sedimentation processes and climate signal preservation in varve structure and chemical composition. This eutrophic and hardwater lake exhibits well preserved biogenic varves with high sedimentation rates (5-8 mm/yr). We conducted a three-year long observation of limnological conditions within the water column and recent sediment fluxes as well as analyzed a 70-cm long sediment core from the deepest part of the lake basin covering the last 125 years. Thin sections were prepared and analyzed microscopically for individual laminae composition. We also measured chemical variability within varves using high-resolution XRF scanning of impregnated sediment slabs. We demonstrate that different mixing patterns may occur in Lake Żabińskie, from dimictic to meromictic depending on the meteorological conditions. Sediment fluxes varied substantially during the observation period with characteristic spring maxima and, optionally, a second late fall maxima. Considerable variability was also observed for the fluxes of total organic carbon, biogenic silica and calcite. Microscopic investigation of the topmost sediments revealed a complex varve structure showing a distinct spring calcite lamina followed by one or more fine calcite laminae interbedded with diatom-rich laminae and, finally, by an organic-rich lamina with minerogenic admixtures deposited during winter

  12. Structure determination at room temperature and phase transition studies above $T_c$ in ABi4Ti4O15 (A = Ba, Sr or Pb)

    G Nalini; T N Guru Row


    The room temperature structure of three compounds belonging to the Aurivillius family ( = 4), ABi4Ti4O15 (A = Ba, Sr or Pb) has been analysed. BaBi4Ti4O15 crystallizes in a tetragonal 4/ space group whereas SrBi4Ti4O15 and PbBi4Ti4O15 crystallize in the orthorhombic space group $A2_1am$. The starting model for the Sr and Pb analogues was derived from ab initio methods and refined using the Rietveld method. The cations Ba and Sr are disordered over the Bi sites while the Pb cation is found exclusively in the [Bi2O2]2+ layers. The TiO6 octahedra are tilted with the Ti–O bonds forming zigzag chains along the `’ axis. The displacement of Bi atoms along the `’ axis might be responsible for ferroelectricity in these compounds. The high temperature X-ray data above $T_c$ indicate no structural transition for A = Ba and Pb while A = Sr transforms to the tetragonal structure.

  13. Mesure des stades de developpement du sapin baumier (Abies balsamea [L.] Mill.) par teledetection des maxima locaux sur images a tres haute resolution

    Demers, Alain

    L'objectif principal de cette these est d'identifier les parametres cles pour lier le developpement morphologique de la cime du sapin baumier (Abies balsamea [L.] Mill.) aux patrons de reflectance qu'elle genere sur une image aerienne a haute resolution. La quantite et la distribution spatiale des aiguilles dans le sapin dependent de la structure des branches. Un mecanisme de ramification particulier (la reiteration) ressort comme le parametre cle pour lier l'augmentation de la biomasse foliaire et la forme de la branche. Les reiterats forment des agglomerats de feuillage qui engendrent des inegalites dans les patrons de reflectance de la cime. La reflectance est plus elevee ou il y a une forte concentration de reiterats (maximum local). On demontre que le developpement du sapin baumier se mesure au moyen de l'analyse des maxima locaux de la cime sur une image. La biomasse de l'arbre se mesure par la teledetection et l'analyse des patrons de reflectance de la cime, c'est-a-dire la mesure du niveau de reiteration par le decompte des maxima locaux de la cime. Cette etude demontre qu'il est possible de proceder a l'inventaire du volume de bois d'un peuplement par la teledetection de l'architecture des arbres.

  14. Impact of climate change, seedling type and provenance on the risk of damage to Norway spruce (Picea abies (L.) Karst.) seedlings in Sweden due to early summer frosts

    Langvall, Ola (Swedish Univ. of Agricultural Sciences, Unit for Field-based Forest Research, Asa Forest Research Station, Lammhult (Sweden))


    A model including site-specific microclimate-affecting properties of a forest regeneration area together with seedling characteristics was used to evaluate the accumulated risk of frost damage to Norway spruce (Picea abies (L.) Karst.) seedlings. Climate change in Sweden was simulated on the basis of the regional climate model RCA3. The daily average temperature, the driving factor for bud burst in the model, was adjusted using the difference between the mean of the climate model data for the years 1961-1990 and 2036-2065. The model was run for a highly frost prone, clear-cut site in which bare-rooted Norway spruce seedlings of mid-Swedish provenance were planted. Alternate runs were conducted with data for containerized seedlings and seedlings of Belarusian origin. The study showed that bud burst will occur at earlier dates throughout Sweden in the period 2036-2065 if the climate changes according to either of the climate scenarios examined, compared to the reference period 1961-1990. Furthermore, the risk of damage to Norway spruce seedlings as a result of frost events during summer will increase in southern Sweden and be unaffected or decrease in northern Sweden. The risk of frost damage was exacerbated in containerized seedlings, while the risk was lower for the seedlings of Belarusian provenance when compared with bare-rooted seedlings or seedlings of mid-Swedish origin

  15. Functions for biomass and basic density of stem, crown and root system of Norway spruce (Picea abies (L.) Karst.) in Denmark

    Skovsgaard, Jens Peter (Swedish Univ. of Agricultural Sciences (SLU), Southern Swedish Forest Research Centre, Alnarp (Sweden)); Bald, Caroline (Danish Nature Agency, Koebenhavn Oe (Denmark)); Nord-Larsen, Thomas (Forest and Landscape, Univ. of Copenhagen, Frederiksberg (Denmark))


    Models for predicting the biomass of forest trees are becoming increasingly important for assessing forest resources and carbon sequestration in forests. We developed functions for predicting the biomass and basic density of above and below ground parts of Norway spruce (Picea abies (L.) Karst.) in Denmark. Separate models were developed for branches (including foliage), stem and the below-ground stump and root system as well as for the aggregate components of total above-ground biomass and total tree biomass. Trees were sampled in 14 forest stands, reflecting the range of growth conditions and thinning practises of Norway spruce in Denmark. Because of inclusion of experimental plots, data reflected a wider range of thinning practices than commonly used in forestry practice. The data included measurements of biomass and basic density from 114 trees, two of which were regarded as outliers and consequently excluded in the final model estimation. The final models reflected known properties of tree growth and allocation of biomass among different tree components of even-aged Norway spruce. The models were successful in predicting biomass, basic density and biomass expansion factors across a wide variety of tree sizes, stand treatments and growth conditions. The models are believed to substantially improve national estimates of carbon sequestration and biomass resources

  16. An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems

    Verschraegen Gerda


    Full Text Available Abstract Background Currently, most laboratories identify yeasts routinely on the basis of morphology and biochemical reactivity. This approach has quite often limited discriminatory power and may require long incubation periods. Due to the increase of fungal infections and due to specific antifungal resistence patterns for different species, accurate and rapid identification has become more important. Several molecular techniques have been described for fast and reliable identification of yeast isolates, but interlaboratory exchangeability of identification schemes of molecular techniques has hardly been studied. Here, we compared amplified ITS2 fragment length determination by an ABI Prism 310 (Applied Biosystems, Foster City, Ca. capillary electrophoresis system with that obtained by a CEQ8000 (Beckman Coulter, Fullerton, Ca. capillary electrophoresis system. Results Although ITS2 size estimations on both systems differed and separate libraries had to be constructed for each system, both approaches had the same discriminatory power with regard to the 44 reference strains, identical identifications were obtained for 39/ 40 clinical isolates in both laboratories and strains from 51 samples were correctly identified using CEQ8000, when compared to phenotypic identification. Conclusion Identification of yeasts with ITS2-PCR followed by fragment analysis can be carried out on different capillary electrophoresis systems with comparable discriminatory power.

  17. Updating beliefs and combining evidence in adaptive forest management under climate change: a case study of Norway spruce (Picea abies L. Karst) in the Black Forest, Germany.

    Yousefpour, Rasoul; Temperli, Christian; Bugmann, Harald; Elkin, Che; Hanewinkel, Marc; Meilby, Henrik; Jacobsen, Jette Bredahl; Thorsen, Bo Jellesmark


    We study climate uncertainty and how managers' beliefs about climate change develop and influence their decisions. We develop an approach for updating knowledge and beliefs based on the observation of forest and climate variables and illustrate its application for the adaptive management of an even-aged Norway spruce (Picea abies L. Karst) forest in the Black Forest, Germany. We simulated forest development under a range of climate change scenarios and forest management alternatives. Our analysis used Bayesian updating and Dempster's rule of combination to simulate how observations of climate and forest variables may influence a decision maker's beliefs about climate development and thereby management decisions. While forest managers may be inclined to rely on observed forest variables to infer climate change and impacts, we found that observation of climate state, e.g. temperature or precipitation is superior for updating beliefs and supporting decision-making. However, with little conflict among information sources, the strongest evidence would be offered by a combination of at least two informative variables, e.g., temperature and precipitation. The success of adaptive forest management depends on when managers switch to forward-looking management schemes. Thus, robust climate adaptation policies may depend crucially on a better understanding of what factors influence managers' belief in climate change.

  18. Distribution of lignin and its coniferyl alcohol and coniferyl aldehyde groups in Picea abies and Pinus sylvestris as observed by Raman imaging.

    Hänninen, Tuomas; Kontturi, Eero; Vuorinen, Tapani


    Wood cell wall consists of several structural components, such as cellulose, hemicelluloses and lignin, whose concentrations vary throughout the cell wall. It is a composite where semicrystalline cellulose fibrils, acting as reinforcement, are bound together by amorphous hemicelluloses and lignin matrix. Understanding the distribution of these components and their functions within the cell wall can provide useful information on the biosynthesis of trees. Raman imaging enables us to study chemistry of cell wall without altering the structure by staining the sample or fractionating it. Raman imaging has been used to analyze distributions of lignin and cellulose, as well as the functional groups of lignin in wood. In our study, we observed the distribution of cellulose and lignin, as well as the amount of coniferyl alcohol and aldehyde groups compared to the total amount of lignin in pine (Pinus sylvestris) and spruce (Picea abies) wood samples. No significant differences could be seen in lignin and cellulose distribution between these samples, while clear distinction was observed in the distribution of coniferyl alcohols and coniferyl aldehyde in them. These results could provide valuable insight on how two similar wood species control biosynthesis of lignin differently during the differentiation of cell wall.

  19. Effects of season and urea treatment on infection of stumps of Picea abies by Heterobasidion annosum in stands on former arable land

    Brandtberg, P.O. [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Ecology and Environmental Research; Johansson, Martin [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Mycology and Pathology; Seeger, P. [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Statistics


    Between 1986 and 1990, a series of thinnings were made in previously unthinned first rotation stands on former arable land located in the southern half of Sweden. The aim was to evaluate the effects of season and urea treatment on the frequency of infection of stumps of Norway spruce (Picea abies (L.) Karst.) by the root-rot fungus Heterobasidion annosum (Fr.) Bref. Untreated stumps, resulting from 60 thinnings (22-100 stumps each, altogether ca 3000 stumps) made at different times of year, were investigated 3-24 months after cutting to determine whether they were infected with H. annosum. On average only 2% of the stumps from thinnings made in November-February were infected, whereas the incidence of infection among stumps thinned in June-July was 34%. Two methods of treating stumps with urea to prevent stump infection by H. annosum after thinning were evaluated in terms of effectiveness. The freshly cut stumps were treated with a 20% urea solution, transformed to a gel by adding 0.2% carboxymethyl cellulose, or with a 30% urea solution. On average, the reduction in infection rate obtained was 62% with the first method and 85% with the latter. In a separate study involving a concentration series of urea, there was a considerable drop in protection efficiency, from 89% to 58%, when the concentration was decreased from 30% to 15%. 38 refs, 3 figs, 1 tab

  20. Bio-Guided Isolation of Methanol-Soluble Metabolites of Common Spruce (Picea abies Bark by-Products and Investigation of Their Dermo-Cosmetic Properties

    Apostolis Angelis


    Full Text Available Common spruce (Picea abies L. is a fast-growing coniferous tree, widely used in several countries for the production of sawn wood, timber and pulp. During this industrial exploitation, large quantities of barks are generated as waste materials. The aim of this study was the bio-guided investigation and the effective recovery of methanol-soluble metabolites of common spruce bark for the development of new dermo-cosmetic agents. The active methanol extract was initially fractionated by Centrifugal Partition Chromatography (CPC using a triphasic solvent system in a step-gradient elution mode. All resulting fractions were evaluated for their antibacterial activity, antioxidant activity and their capability to inhibit tyrosinase, elastase and collagenase activity. In parallel, the chemical composition of each fraction was established by combining a 13C-NMR dereplication approach and 2D-NMR analyses. As a result, fourteen secondary metabolites corresponding to stilbene, flavonoid and phenolic acid derivatives were directly identified in the CPC fractions. A high amount (0.93 g of E-astringin was recovered from 3 g of crude extract in a single 125 min run. E-Astringin significantly induced the tyrosinase activity while E-piceid, taxifolin, and taxifolin-3′-O-glucopyranoside exhibited significant anti-tyrosinase activity. The above compounds showed important anti-collagenase and antimicrobial activities, thus providing new perspectives for potential applications as cosmetic ingredients.

  1. Nutrients induction on lead, cadmium, manganese, zinc and cobalt speciation in the sediments of Aby lagoon (Côte d'Ivoire

    Akpétou K. L.,


    Full Text Available This study reported nitrogen and phosphorus leverage on lead, Cadmium, Manganese, Zinc and Cobalt speciation in the Aby lagoon sediments. The trace elements and water samples were collected from eleven sites located within the four lagoon sectors. Sequential extraction was carried out in five fractions: exchangeable (F1, bound to the carbonates (F2, bound to iron and manganese (oxy hydroxides (F3, bound to the organic matters (F4 and residual(F5. Heavy metals chemical fractionation followed the four-step Zerbe and al. (1999 process, completed with an acid digestion method for residual (F5 extraction. Co-inertia analysis monitored with ADE4 package showed that nitrite and Kjeldhal nitrogen (TKN mainly influenced Co speciation and especially the compounds (total lead, total Cd, total Zn, Co-F1 and Co-F4 tend to accumulate on sediment surface. However, their higher influence than nitrates on previous metals was too lower than the other nitrogen compounds which they were very negatively correlated. As the phosphorus compounds which showed a greatest impact on lead, Cd, Mn, and Zn speciation. The respective resulted factorial values were about -1 and 1. Total and inorganic phosphorus portion showed a similar influence range than total nitrogen on the various studied heavy metals speciation in sediment.

  2. The seasonal variation of arthropods living on forest soil at different altitudes in fir (Abies nordmanniana subsp. bornmulleriana ecosystem in Bolu-Aladağ

    Ahmet Duyar


    Full Text Available In the forest ecosystems, soil arthropods (Arthropoda, as primary and secondary consumers, have a significant role in litter decay and decomposition processes. The abundance, diversity and community structure of arthropods in soil ecosystem; give rapid response to change of site characteristics. The current study was aimed to determine of seasonal variation of soil arthropods on forest floor at different altitudes in Uludağ Fir (Abies nordmanniana subsp. bornmulleriana Mattf. ecosystem which is an important forest tree species in Turkey. The study was conducted in pure fir stands at 1200-1600 m altitudes (4 elevation gradients in Aladağ, Bolu. The sampling was carried out for each winter, spring, summer and autumn seasons. The samples were collected from forest floor by pitfall traps. Variations of abundance and diversity of arthropods were evaluated according to seasons and altitudes. The distributions in trophic levels and biological diversity of arthropods were also determined. During the study, the maximum abundance of arthropods was 7576 individuals/m² in summer among seasons, and was 7854 individuals/m² at 1200 m altitude. Shannon-Wiener Index (H′ and Species Richness (S′ values were detected in the pitfall traps (H′= 2.22; S′= 22.

  3. 神农架巴山冷杉林土壤碳储量特征%Characteristics of Soil Carbon Storage of Abies fargesii Forest in Shennongjia

    崔鸿侠; 肖文发; 潘磊; 黄志霖; 王晓荣; 庞宏东


    The natural forest, secondary forest and plantation plots of Abies fargesii were selected in the Shennongjia nature reserve, and soil carbon storage of the three forest types was analyzed. The result showed litter storage of the natural forest, secondary forest and plantation was 97. 15 t·hm-2 , 53. 03 t·hm-2 and 83. 22 t·hm-2 , respectively. The content of the soil organic carbon gradually declined with the soil depth from 0 to 60 cm, and the mean soil organic carbon storage was 53. 31 g·kg-1 for natural forest > 48. 66 g·kg-1 for secondary forest > 47. 62 g·kg-1 for plantation. The total soil organic carbon storage was 271.93 t·hm-2 for natural forest > 219.80 t·hm-2 for plantation > 218.29 t·hm-2 for secondary forest. There was a negative significant correlation between total soil organic carbon storage and Shannon-Wiener diversity index of the shrub and herb layers, and the soil carbon storage from 0 to 40 cm layers was significantly positive correlated with the litter storage.

  4. Chemical composition and biological activity of Abies alba and A. koreana seed and cone essential oils and characterization of their seed hydrolates.

    Wajs-Bonikowska, Anna; Sienkiewicz, Monika; Stobiecka, Agnieszka; Maciąg, Agnieszka; Szoka, Łukasz; Karna, Ewa


    The chemical composition, including the enantiomeric excess of the main terpenes, the antimicrobial and antiradical activities, as well as the cytotoxicity of Abies alba and A. koreana seed and cone essential oils were investigated. Additionally, their seed hydrolates were characterized. In the examined oils and hydrolates, a total of 174 compounds were identified, which comprised 95.6-99.9% of the volatiles. The essential oils were mainly composed of monoterpene hydrocarbons, whereas the composition of the hydrolates, differing from the seed oils of the corresponding fir species, consisted mainly of oxygenated derivatives of sesquiterpenes. The seed and cone essential oils of both firs exhibited DPPH-radical-scavenging properties and low antibacterial activity against the bacterial strains tested. Moreover, they evoked only low cytotoxicity towards normal fibroblasts and the two cancer cell lines MCF-7 and MDA-MBA-231. At concentrations up to 50 μg/ml, all essential oils were safe in relation to normal fibroblasts. Although they induced cytotoxicity towards the cancer cells at concentrations slightly lower than those required for the inhibition of fibroblast proliferation, their influence on cancer cells was weak, with IC50 values similar to those observed towards normal fibroblasts.

  5. WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration.

    Xie, Min-Jue; Yagi, Hideshi; Kuroda, Kazuki; Wang, Chen-Chi; Komada, Munekazu; Zhao, Hong; Sakakibara, Akira; Miyata, Takaki; Nagata, Koh-Ichi; Oka, Yuichiro; Iguchi, Tokuichi; Sato, Makoto


    Glia-guided migration (glia-guided locomotion) during radial migration is a characteristic yet unique mode of migration. In this process, the directionality of migration is predetermined by glial processes and not by growth cones. Prior to the initiation of glia-guided migration, migrating neurons transform from multipolar to bipolar, but the molecular mechanisms underlying this multipolar-bipolar transition and the commencement of glia-guided migration are not fully understood. Here, we demonstrate that the multipolar-bipolar transition is not solely a cell autonomous event; instead, the interaction of growth cones with glial processes plays an essential role. Time-lapse imaging with lattice assays reveals the importance of vigorously active growth cones in searching for appropriate glial scaffolds, completing the transition, and initiating glia-guided migration. These growth cone activities are regulated by Abl kinase and Cdk5 via WAVE2-Abi2 through the phosphorylation of tyrosine 150 and serine 137 of WAVE2. Neurons that do not display such growth cone activities are mispositioned in a more superficial location in the neocortex, suggesting the significance of growth cones for the final location of the neurons. This process occurs in spite of the "inside-out" principle in which later-born neurons are situated more superficially.

  6. Early needle senescence and thinning of the crown structure of Picea abies as induced by chronic SO{sub 2} pollution. 1. Model deduction and analysis

    Slovik, S. [Universitaet Wuerzburg, Wuerzburg (Germany). Julius von Sachs Inst. fuer Biowissenschaften


    Regarding time ranges of years, a rationale has been developed which is capable of explaining `spruce decline` symptoms observed when spruce is exposed to air containing ambient levels of SO{sub 2}. It integrates and interrelates (1) ecophysiological data (tree morphology, assimilate partitioning, canopy turnover, senescence physiology, stomatal conductance, canopy throughfall, sulphur metabolism, tonoplast symport), (2) pedological data (soil leaching, cation recycling, litter decomposition, forest nutrition), and (3) meteorological data (site elevation, length of the annual trunk growth period, SO{sub 2}-pollution). It can also explain field observations at numerous sites of spruce decline in central Europe where SO{sub 2} is implicated as a factor of forest decline: (1) thinning of the canopy structure; (2) early needle senescence; (3) cation deficiency; (4) low SO{sub 2} tolerance at sites with depleted soils in the mountains; (5) synergism of SO{sub 2} pollution and acidic precipitation; (6) recovery after liming, fertilization and after decreasing SO{sub 2} pollution; and (7) higher SO{sub 2} tolerances of deciduous angiosperms. Different SO{sub 2} tolerance strategies are identified. Ecophysiological SO{sub 2} tolerance factors interact in a complex synergistic or antagonistic manner. It is concluded that chronic SO{sub 2} pollution at ambient concentrations predisposes evergreen gymnosperms to suffer under synergistic environmental stresses (frost, drought, pathogenes, etc.). Thinning of the crown structure is massive at extreme sites, where several stresses act simultaneously on the trees. This universal rationale, which is applicable to all tree species, is exemplified here for Norway spruce (Picea abies).

  7. Drought tolerance established by enhanced expression of the CC-NBS-LRR gene, ADR1, requires salicylic acid, EDS1 and ABI1.

    Chini, Andrea; Grant, John J; Seki, Motoaki; Shinozaki, Kazuo; Loake, Gary J


    An activation-tagged allele of activated disease resistance 1 (ADR1) has previously been shown to convey broad spectrum disease resistance. ADR1 was found to encode a coiled-coil (CC)-nucleotide-binding site (NBS)-leucine-rich repeat (LRR) protein, which possessed domains of homology with serine/threonine protein kinases. Here, we show that either constitutive or conditional enhanced expression of ADR1 conferred significant drought tolerance. This was not a general feature of defence-related mutants because cir (constitutive induced resistance)1, cir2 and cpr (constitutive expressor of PR genes)1, which constitutively express systemic acquired resistance (SAR), failed to exhibit this phenotype. Cross-tolerance was not a characteristic of adr1 plants, rather they showed increased sensitivity to thermal and salinity stress. Hence, adr1-activated signalling may antagonise some stress responses. Northern analysis of abiotic marker genes revealed that dehydration-responsive element (DRE)B2A but not DREB1A, RD (response to dehydration)29A or RD22 was expressed in adr1 plant lines. Furthermore, DREB2A expression was salicylic acid (SA) dependent but NPR (non-expressor of PR genes)1 independent. In adr1/ADR1 nahG (naphthalene hydroxylase G), adr1/ADR1 eds (enhanced disease susceptibility)1 and adr1/ADR1 abi1 double mutants, drought tolerance was significantly reduced. Microarray analyses of plants containing a conditional adr1 allele demonstrated that a significant number of the upregulated genes had been previously implicated in responses to dehydration. Therefore, biotic and abiotic signalling pathways may share multiple nodes and their outputs may have significant functional overlap.

  8. Resilience, rapid transitions and regime shifts: fingerprinting the responses of Lake Żabińskie (NE Poland) to climate variability and human disturbance since 1000 AD

    Tylmann, Wojciech; Hernández-Almeida, Iván; Grosjean, Martin; José Gómez Navarro, Juan; Larocque-Tobler, Isabelle; Bonk, Alicja; Enters, Dirk; Ustrzycka, Alicja; Piotrowska, Natalia; Przybylak, Rajmund; Wacnik, Agnieszka; Witak, Małgorzata


    Rapid ecosystem transitions and adverse effects on ecosystem services as responses to combined climate and human impacts are of major concern. Yet few quantitative observational data exist, particularly for ecosystems that have a long history of human intervention. Here, we combine quantitative summer and winter climate reconstructions, climate model simulations and proxies for three major environmental pressures (land use, nutrients and erosion) to explore the system dynamics, resilience, and the role of disturbance regimes in varved eutrophic Lake Żabińskie since AD 1000. Comparison between regional and global climate simulations and quantitative climate reconstructions indicate that proxy data capture noticeably natural forced climate variability, while internal variability appears as the dominant source of climate variability in the climate model simulations during most parts of the last millennium. Using different multivariate analyses and change point detection techniques, we identify ecosystem changes through time and shifts between rather stable states and highly variable ones, as expressed by the proxies for land-use, erosion and productivity in the lake. Prior to AD 1600, the lake ecosystem was characterized by a high stability and resilience against considerable observed natural climate variability. In contrast, lake-ecosystem conditions started to fluctuate at high frequency across a broad range of states after AD 1600. The period AD 1748-1868 represents the phase with the strongest human disturbance of the ecosystem. Analyses of the frequency of change points in the multi-proxy dataset suggests that the last 400 years were highly variable and flickering with increasing vulnerability of the ecosystem to the combined effects of climate variability and anthropogenic disturbances. This led to significant rapid ecosystem transformations.

  9. Effect of Forest Management of Picea abies and Fagus sylvatica with Different Types of Felling on Carbon and Economic Balances in the Czech Republic

    Plch, Radek; Pulkrab, Karel; Bukáček, Jan; Sloup, Roman; Cudlín, Pavel


    The selection of the most sustainable forest management under given site conditions needs suitable criteria and indicators. For this purpose, carbon and economic balance assessment, completed with environmental impact computation using the Life Cycle Assessment (LCA) were used. The aim of this study was to compare forestry operations and wood production of selected forest stands with different i) tree species composition (Norway spruce - Picea abies and European beech - Fagus sylvatica) and ii) type of felling (chainsaw and harvester). Carbon and economic balance methods consist in the comparison of quantified inputs (fossil fuels, electricity, used machinery, fertilizers, etc., converted into emission units of carbon in Mg of C- CO2-eq. or EUR) with quantified outputs (biomass production in Mg of carbon or EUR). In this contribution, similar forest stands (“forest site complexes”) in the 4th forest vegetation zone (in the Czech Republic approximately 400-700 m above sea-level) were selected. Forestry operations were divided into 5 main stages: i) seedling production, ii) stand establishment and pruning, iii) thinning and final cutting, iv) skidding, and v) secondary timber transport and modelled for one rotation period of timber production (ca. 100 years). The differences between Norway spruce and European beech forest stands in the carbon efficiency were relatively small while higher differences were achieved in the economic efficiency (forest stands with Norway spruce had a higher economic efficiency). Concerning the comparison of different types of felling in Norway spruce forest stands, the harvester use proved to induce significantly higher environmental impacts (emission of carbon) and lower economic costs. The comparison of forestry operation stages showed that the main part of carbon emissions, originating from fuel production and combustion, is connected with a thinning and final cutting, skidding and secondary timber transport in relations to

  10. Throughfall deposition and canopy exchange processes along a vertical gradient within the canopy of beech (Fagus sylvatica L.) and Norway spruce (Picea abies (L.) Karst).

    Adriaenssens, Sandy; Hansen, Karin; Staelens, Jeroen; Wuyts, Karen; De Schrijver, An; Baeten, Lander; Boeckx, Pascal; Samson, Roeland; Verheyen, Kris


    To assess the impact of air pollution on forest ecosystems, the canopy is usually considered as a constant single layer in interaction with the atmosphere and incident rain, which could influence the measurement accuracy. In this study the variation of througfall deposition and derived dry deposition and canopy exchange were studied along a vertical gradient in the canopy of one European beech (Fagus sylvatica L.) tree and two Norway spruce (Picea abies (L.) Karst) trees. Throughfall and net throughfall deposition of all ions other than H(+) increased significantly with canopy depth in the middle and lower canopy of the beech tree and in the whole canopy of the spruce trees. Moreover, throughfall and net throughfall of all ions in the spruce canopy decreased with increasing distance to the trunk. Dry deposition occurred mainly in the upper canopy and was highest during the growing season for H(+), NH(4)(+), NO(3)(-) and highest during the dormant season for Na(+), Cl(-), SO(4)(2-) (beech and spruce) and K(+), Ca(2+) and Mg(2+) (spruce only). Canopy leaching of K(+), Ca(2+) and Mg(2+) was observed at all canopy levels and was higher for the beech tree compared to the spruce trees. Canopy uptake of inorganic nitrogen and H(+) occurred mainly in the upper canopy, although significant canopy uptake was found in the middle canopy as well. Canopy exchange was always higher during the growing season compared to the dormant season. This spatial and temporal variation indicates that biogeochemical deposition models would benefit from a multilayer approach for shade-tolerant tree species such as beech and spruce.

  11. Spatial Pattern of the Mitochondrial and Chloroplast Genetic Variation in Poland as a Result of the Migration of Abies alba Mill. from Different Glacial Refugia

    Monika Litkowiec


    Full Text Available Currently, the information on the gene pool of silver fir (Abies alba Mill. at the northeastern edge of its distribution in Poland is scarce and insufficient. Using the advantage provided by markers with different modes of inheritance, a hypothesis that gene flow via both seeds and pollen contributed to the genetic structure across the entire analyzed region was investigated. The geographic distribution of maternally inherited mitochondrial DNA (mtDNA, nad5-4 and paternally inherited chloroplast DNA (cpDNA, psbC variation was studied in 81 Polish populations and three reference populations from Ukraine and Romania. The spatial pattern of mtDNA haplotypes (dispersed via seeds indicated that the Apennine Peninsula was the only maternal glacial refugium for the entire territory of Poland and also the Ukraine no 1 population, whereas the other two populations—Ukraine no 2 and Romania—had the haplotype representing the Balkan origin. By contrast, the cpDNA haplotypes (dispersed via pollen from all studied Polish and reference populations showed that A. alba colonized the current natural range from two genetically distinct glacial refugia located on the Apennine and Balkan peninsulas. The occurrence of cpDNA haplotypes varied among the studied populations. Additionally, statistical analyses were used to infer the genetic structure of examined populations. Two distinct groups of A. alba populations were identified showing the postglacial geographic distribution of haplotypes of both mtDNA and cpDNA. A. alba is an important ecological and economic component of forest ecosystems in Europe. An understanding of the Holocene history of this species is relevant for planning sustainable forest management, and acquired data can contribute to strategies of conservation and restoration.

  12. Cardiomyopathy in ostriches (Struthio camelus) due to avocado (Persea americana var. guatemalensis) intoxication.

    Burger, W P; Naudé, T W; Van Rensburg, I B; Botha, C J; Pienaar, A C


    Nine out of 120 ostriches died from congestive heart failure within 96 h of ingesting avocado leaves and immature fruit in an avocado orchard containing Hass and Fuerte cultivars. Foliage and immature fruit from both cultivars dosed to ostriches (n = 4) and domestic hens (n = 8) resulted in severe cardiomyopathy in all the ostriches. In the hens, which had received a lower dose, milder lesions occurred. Macroscopically the intoxication in ostriches resulted in a severe anasarca of the neck and ventral body. The cardiomyopathy was characterised by degeneration and necrosis of myocytes, a marked infiltration of heterophils and in one case, early fibroplasia.

  13. Distribución vertical de colémbolos muscícolas en un bosque de Abies religiosa del Estado de México, México Vertical distribution of springtails from epiphytic moss from Abies religiosa forest in State of Mexico, Mexico

    Leopoldo Q. Cutz-Pool


    Full Text Available Se estudió la distribución de colémbolos asociados a musgos epífitos, considerando 3 diferentes alturas sobre el árbol soporte: 0 m, 1 m y 2 m. El estudio se realizó en la localidad de San Rafael, Estado de México, en un bosque de Abies religiosa. Los ejemplares se recolectaron en noviembre de 2003, y en marzo, junio y agosto de 2004. En total fueron registradas 12 especies; se estimó el índice de diversidad de Shannon, la riqueza de especies, y la densidad y diversidad de colémbolos en 3 comunidades diferentes. La riqueza y densidad disminuyeron conforme la altura sobre los árboles se incrementó. La altura sobre los árboles tuvo un efecto significativamente negativo sobre la diversidad así como en la densidad de 4 especies: Pseudachorutes subcrassus, Entomobrya ca. triangularis, Americabrya arida y Ptenothrix marmorata. Los resultados sugieren que la estratificación vertical de los colémbolos muscícolas está afectada de manera diferencial por los microhábitats que se presentan sobre los árboles soporte a diversas alturas, así como por las características morfológicas de las especies. Estos resultados demuestran la capacidad de los colémbolos para migraciones verticales en busca de alimento y para evitar la depredación.We evaluated the Collembola communities at 3 different heights on epiphytic moss. The study was carried out in an Abies religiosa forest, in the locality of San Rafael in the State of Mexico, Mexico. Collembola inhabiting epiphytic moss were sampled during November 2003, and during March, June and August 2004. The Shannon's diversity index, species richness, as well as their density and diversity were estimated in 3 communities. The total number of Collembola species that was collected was 12, with the species richness and density decreasing as the height of tree increased. The height on trees where moss was sampled had a significant negative effect on species diversity as well as on the densities of

  14. 中国冷杉属植物的地理分布特征及成因初探%Geographical distribution characters and generating mechanisms of genus Abies in China

    王清春; 李晖; 李晓笑


    By combining existing documents and literatures and field investigation data, the geographical distribution and distribution pattern of species diversity of fir(Abies Mill.) in China were quantitatively analyzed, and the endangerment mechanisms of several rare and endangered species were also discussed. The results show that (1) the Hengduan mountains regions in West Sichuan and North Yunnan were the modern distribution center and mutation center of fir in China; (2)With the increase of latitude and longitude, the distribution pattern of species diversity of fir presented an rising trend that firstly increases and then decreases, between 98 ° to 104 ° E and 26 ° to 30 ° N, the distribution of species was the most concentrated; the vertical distribution pattern presented like the horizontal distribution pattern. Between 2600 m to 3600 m, there were more species number; (3) the distribution altitude of Abies increased with a decrease of longitude and decreases with an increase of latitude; (4)the needs of wet and cold habitat, and climate change were the reasons that caused several species of Abies to be endangered.%结合文献资料和野外调查,定量分析了中国冷杉属Abies Mill.植物的地理分布特征和物种多样性分布格局,并探讨几种珍稀濒危冷杉属植物孤岛状分布的成因.结果表明:(1)川西滇北横断山区是我国冷杉属的现代分布中心和变异中心;(2)随着经度、纬度的增加,冷杉属植物物种多样性呈现先增加后减少的分布格局,在98°~104°E、26°~30°N之间,冷杉属种数分布最集中;同样,垂直分布格局也呈现相似的趋势,在2 600~3600m的中高海拔冷杉物种数较多; (3)冷杉属植物分布的海拔高度随经度的减小而上升,随纬度的增加而降低; (4)冷杉属植物喜湿冷的特性和气候变化等原因导致该属几种冷杉的濒危现状.

  15. Impacto de la senescencia foliar sobre la producción de fotoasimilados y el rendimiento de maíz (Zea mays L.) bajo condiciones de estrés abiótico

    Antonietta, Mariana A.


    El objetivo general de esta tesis fue estudiar si la demora en la senescencia foliar (carácter stay-green, SG) en híbridos de maíz confiere tolerancia del rendimiento al estrés abiótico. Se realizaron ensayos a campo utilizando híbridos contrastantes en la senescencia foliar. Se registró la evolución de la senescencia, la producción neta de fotoasimilados, el rendimiento y sus componentes. Bajo sequía en post-floración, los genotipos SG presentaron mayor producción de fotoasimilados y esto se...

  16. Nuevos mecanismos moleculares de tolerancia a sequía y otros tipos de estrés abiótico en especies arbóreas de interés económico

    Contreras Mogollón, Ángela Bibiana


    Las masas forestales tienen una importancia colosal para nuestra sociedad y el conjunto de la biosfera. Estudios recientes a escala mundial indican que la sequía es el factor abiótico que más afecta a su crecimiento y supervivencia, seguida por las temperaturas extremas y la salinidad. Aunque comprender los mecanismos con que las especies arbóreas toleran estas formas de estrés tiene un interés aplicado evidente, dichos mecanismos se han estudiado mucho más en especies herbáceas modelo o de i...

  17. Sequencing of the needle transcriptome from Norway spruce (Picea abies Karst L. reveals lower substitution rates, but similar selective constraints in gymnosperms and angiosperms

    Chen Jun


    Full Text Available Abstract Background A detailed knowledge about spatial and temporal gene expression is important for understanding both the function of genes and their evolution. For the vast majority of species, transcriptomes are still largely uncharacterized and even in those where substantial information is available it is often in the form of partially sequenced transcriptomes. With the development of next generation sequencing, a single experiment can now simultaneously identify the transcribed part of a species genome and estimate levels of gene expression. Results mRNA from actively growing needles of Norway spruce (Picea abies was sequenced using next generation sequencing technology. In total, close to 70 million fragments with a length of 76 bp were sequenced resulting in 5 Gbp of raw data. A de novo assembly of these reads, together with publicly available expressed sequence tag (EST data from Norway spruce, was used to create a reference transcriptome. Of the 38,419 PUTs (putative unique transcripts longer than 150 bp in this reference assembly, 83.5% show similarity to ESTs from other spruce species and of the remaining PUTs, 3,704 show similarity to protein sequences from other plant species, leaving 4,167 PUTs with limited similarity to currently available plant proteins. By predicting coding frames and comparing not only the Norway spruce PUTs, but also PUTs from the close relatives Picea glauca and Picea sitchensis to both Pinus taeda and Taxus mairei, we obtained estimates of synonymous and non-synonymous divergence among conifer species. In addition, we detected close to 15,000 SNPs of high quality and estimated gene expression differences between samples collected under dark and light conditions. Conclusions Our study yielded a large number of single nucleotide polymorphisms as well as estimates of gene expression on transcriptome scale. In agreement with a recent study we find that the synonymous substitution rate per year (0.6 × 10

  18. Monsoon Season Moisture Deficit Limits Growth in Co-Occurring Alpine Shrub (Cassiope fastigata) and Tree (Abies spectabilis) Species in the Central Himalayas, Nepal

    Rayback, S. A.; Shrestha, K. B.; Hofgaard, A.


    Recent evidence indicates changing climatological conditions in the Nepalese Himalayas including decreasing precipitation, a weakening Indian monsoon and rising temperatures. Trees and shrubs found at treeline are considered to be highly sensitive to climate, but the climatic effects on these ecotone species in the Himalayas are not well understood. Dendrochronological techniques applied to co-occurring shrubs and trees up-and down-slope of treeline extend our understanding of vegetation response at range margins and into tree-less environments. We developed tree-ring width and annual height increment chronologies for Abies spectabilis (Himalayan fir) and the first annual growth increment and annual production of leaves chronologies for Cassiope fastigata (Himalayan heather) at a high elevation site in central Nepal. C. fastigata chronologies showed moisture availability in late pre-monsoon and monsoon seasons of the previous year are critical to stem elongation and leaf production (AGI and previous May-August SPEI-12, r = 0.790; LEAF and previous June-September SPEI-12, r = 0.708) A. spectabilis chronologies were significantly and negatively correlated with monsoon season temperature during the current year (tree-ring width and June mean temperature, r = -0.677; height-increment and Sept maximum temperature, r = -0.605). In addition to both long-term and recent declines in moisture in the Himalayas, moisture deficit may be further exacerbated at high elevation sites via run-off and higher levels of evapotranspiration resulting in growth reductions, dieback and even death of these species. These results highlight that not all mid-latitude, high elevation treelines are limited by temperature as previously thought and that severe drought stress may initiate downslope treeline retraction. Understanding the response of co-occurring tree and shrub species to climate, now and in the future, may help to elucidate the physiological mechanisms controlling local and

  19. Effects of open-field experimental warming on the growth of two-year-old Pinus densiflora and Abies holophylla seedlings

    Han, S.; Son, Y.; Lee, S.; Jo, W.; Yoon, T.; Park, C.; Ko, S.; Kim, J.; Han, S.; Jung, Y.


    Temperature increase due to climate change is expected to affect tree growth and distribution [Way and Oren, 2010]. The responses of trees to warming vary with tree species, ontogenic stages, tree life forms, and biomes. Especially, seedling stage is a vulnerable period for tree survival and competition [Saxe et al., 2007] and thus research on effects of temperature increase on seedling stage is needed. We aimed to examine the responses of coniferous seedlings to future temperature increase by conducting an open-field warming experiment. An experimental warming set-up using infra-red heater was built in 2011 and the temperature in warming plots has been regulated to 3°C higher than that of control plots constantly. The seeds of Pinus densiflora and Abies holophylla were planted in each 1 m × 1 m plot (n=3) in April, 2012. Seedling growth, root collar diameter (RCD) and height of 45 individuals of each plot were measured in June and July, 2012. The survival rate of seedlings was also measured. Survival rate of P. densiflora was lower in warming plots (93.3%) than in control plots (100.0%, pdensiflora seedlings were not significantly different between control and warming plots, however, height of A. holophylla was significantly higher in warming plots in June and July (p<0.01). Comparatively, RCD of A. holophylla was only higher in control plots in June. While there is still a lack of case studies on the growth of seedlings under experimental warming, a few studies reported increased seedling growth [Yin et al., 2008] or and no difference [Han et al., 2009] in warming plots. Different responses of seedling growth between two species of the current study might be derived from species-specific acclimation to temperature increase and/or other limiting factors [Way and Oren, 2010]. This result is, to our knowledge, unprecedented and will contribute to the knowledge of species-specific growth response of tree species and to development of model predicting species

  20. Applicability of non-destructive substitutes for leaf area in different stands of Norway spruce (Picea abies L. Karst.) focusing on traditional forest crown measures.

    Laubhann, Daniel; Eckmüllner, Otto; Sterba, Hubert


    Since individual tree leaf area is an important measure for productivity as well as for site occupancy, it is of high interest in many studies about forest growth. The exact determination of leaf area is nearly impossible. Thus, a common way to get information about leaf area is to use substitutes. These substitutes are often variables which are collected in a destructive way which is not feasible for long term studies. Therefore, this study aimed at testing the applicability of using substitutes for leaf area which could be collected in a non-destructive way, namely crown surface area and crown projection area. In 8 stands of Norway spruce (Picea abies L. Karst.), divided into three age classes and two thinning treatments, a total of 156 trees were felled in order to test the relationship between leaf area and crown surface area and crown projection area, respectively. Individual tree leaf area of the felled sample trees was estimated by 3P-branch sampling with an accuracy of ±10%. Crown projection area and crown surface area were compared with other, more commonly used, but destructive predictors of leaf area, namely sapwood area at different heights on the bole. Our investigations confirmed findings of several studies that sapwood area is the most precise measure for leaf area because of the high correlation between sapwood area and the leaf area. But behind sapwood area at crown base and sapwood area at three tenth of the tree height the predictive ability of crown surface area was ranked third and even better than that of sapwood area at breast height (R(2) = 0.656 compared with 0.600). Within the stands leaf area is proportional to crown surface area. Using the pooled data of all stands a mixed model approach showed that additionally to crown surface area dominant height and diameter at breast height (dbh) improved the leaf area estimates. Thus, taking dominant height and dbh into account, crown surface area can be recommended for estimating the leaf area

  1. Ground-level ozone differentially affects nitrogen acquisition and allocation in mature European beech (Fagus sylvatica) and Norway spruce (Picea abies) trees.

    Weigt, R B; Häberle, K H; Millard, P; Metzger, U; Ritter, W; Blaschke, H; Göttlein, A; Matyssek, R


    Impacts of elevated ground-level ozone (O(3)) on nitrogen (N) uptake and allocation were studied on mature European beech (Fagus sylvatica L.) and Norway spruce (Picea abies [L.] Karst.) in a forest stand, hypothesizing that: (i) chronically elevated O(3) limits nutrient uptake, and (ii) beech responds more sensitively to elevated O(3) than spruce, as previously found for juvenile trees. Tree canopies were exposed to twice-ambient O(3) concentrations (2 × O(3)) by a free-air fumigation system, with trees under ambient O(3) serving as control. After 5 years of O(3) fumigation, (15)NH(4)(15)NO(3) was applied to soil, and concentrations of newly acquired N (N(labelled)) and total N (N(total)) in plant compartments and soil measured. Under 2 × O(3), N(labelled) and N(total) were increased in the bulk soil and tended to be lower in fine and coarse roots of both species across the soil horizons, supporting hypothesis (i). N(labelled) was reduced in beech foliage by up to 60%, and by up to 50% in buds under 2 × O(3). Similarly, N(labelled) in stem bark and phloem was reduced. No such reduction was observed in spruce, reflecting a stronger effect on N acquisition in beech in accordance with hypothesis (ii). In spruce, 2 × O(3) tended to favour allocation of new N to foliage. N(labelled) in beech foliage correlated with cumulative seasonal transpiration, indicating impaired N acquisition was probably caused by reduced stomatal conductance and, hence, water transport under elevated O(3). Stimulated fine root growth under 2 × O(3) with a possible increase of below-ground N sink strength may also have accounted for lowered N allocation to above-ground organs. Reduced N uptake and altered allocation may enhance the use of stored N for growth, possibly affecting long-term stand nutrition.

  2. Biosorption of B-aflatoxins Using Biomasses Obtained from Formosa Firethorn [Pyracantha koidzumii (Hayata) Rehder].

    Ramales-Valderrama, Rosa Adriana; Vázquez-Durán, Alma; Méndez-Albores, Abraham


    Mycotoxin adsorption onto biomaterials is considered as a promising alternative for decontamination without harmful chemicals. In this research, the adsorption of B-aflatoxins (AFB₁ and AFB₂) using Pyracantha koidzumii biomasses (leaves, berries and the mixture of leaves/berries) from aqueous solutions was explored. The biosorbent was used at 0.5% (w/v) in samples spiked with 100 ng/mL of B-aflatoxin standards and incubated at 40 °C for up to 24 h. A standard biosorption methodology was employed and aflatoxins were quantified by an immunoaffinity column and UPLC methodologies. The biosorbent-aflatoxin interaction mechanism was investigated from a combination of zeta potential (ζ), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The highest aflatoxin uptakes were 86% and 82% at 6 h using leaves and the mixture of leaves/berries biomasses, respectively. A moderate biosorption of 46% was attained when using berries biomass. From kinetic studies, the biosorption process is described using the first order adsorption model. Evidence from FTIR spectra suggests the participation of hydroxyl, amine, carboxyl, amide, phosphate and ketone groups in the biosorption and the mechanism was proposed to be dominated by the electrostatic interaction between the negatively charged functional groups and the positively charged aflatoxin molecules. Biosorption by P. koidzumii biomasses has been demonstrated to be an alternative to conventional systems for B-aflatoxins removal.

  3. Biosorption of B-aflatoxins Using Biomasses Obtained from Formosa Firethorn [Pyracantha koidzumii (Hayata) Rehder

    Ramales-Valderrama, Rosa Adriana; Vázquez-Durán, Alma; Méndez-Albores, Abraham


    Mycotoxin adsorption onto biomaterials is considered as a promising alternative for decontamination without harmful chemicals. In this research, the adsorption of B-aflatoxins (AFB1 and AFB2) using Pyracantha koidzumii biomasses (leaves, berries and the mixture of leaves/berries) from aqueous solutions was explored. The biosorbent was used at 0.5% (w/v) in samples spiked with 100 ng/mL of B-aflatoxin standards and incubated at 40 °C for up to 24 h. A standard biosorption methodology was employed and aflatoxins were quantified by an immunoaffinity column and UPLC methodologies. The biosorbent-aflatoxin interaction mechanism was investigated from a combination of zeta potential (ζ), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The highest aflatoxin uptakes were 86% and 82% at 6 h using leaves and the mixture of leaves/berries biomasses, respectively. A moderate biosorption of 46% was attained when using berries biomass. From kinetic studies, the biosorption process is described using the first order adsorption model. Evidence from FTIR spectra suggests the participation of hydroxyl, amine, carboxyl, amide, phosphate and ketone groups in the biosorption and the mechanism was proposed to be dominated by the electrostatic interaction between the negatively charged functional groups and the positively charged aflatoxin molecules. Biosorption by P. koidzumii biomasses has been demonstrated to be an alternative to conventional systems for B-aflatoxins removal. PMID:27420096

  4. Biosorption of B-aflatoxins Using Biomasses Obtained from Formosa Firethorn [Pyracantha koidzumii (Hayata Rehder

    Rosa Adriana Ramales-Valderrama


    Full Text Available Mycotoxin adsorption onto biomaterials is considered as a promising alternative for decontamination without harmful chemicals. In this research, the adsorption of B-aflatoxins (AFB1 and AFB2 using Pyracantha koidzumii biomasses (leaves, berries and the mixture of leaves/berries from aqueous solutions was explored. The biosorbent was used at 0.5% (w/v in samples spiked with 100 ng/mL of B-aflatoxin standards and incubated at 40 °C for up to 24 h. A standard biosorption methodology was employed and aflatoxins were quantified by an immunoaffinity column and UPLC methodologies. The biosorbent-aflatoxin interaction mechanism was investigated from a combination of zeta potential (ζ, Fourier transform infrared spectroscopy (FTIR and scanning electron microscopy (SEM. The highest aflatoxin uptakes were 86% and 82% at 6 h using leaves and the mixture of leaves/berries biomasses, respectively. A moderate biosorption of 46% was attained when using berries biomass. From kinetic studies, the biosorption process is described using the first order adsorption model. Evidence from FTIR spectra suggests the participation of hydroxyl, amine, carboxyl, amide, phosphate and ketone groups in the biosorption and the mechanism was proposed to be dominated by the electrostatic interaction between the negatively charged functional groups and the positively charged aflatoxin molecules. Biosorption by P. koidzumii biomasses has been demonstrated to be an alternative to conventional systems for B-aflatoxins removal.

  5. Interconnection among Main Tree Populations of Abies yuanbaoshanensis Community%元宝山冷杉群落主要树木种群间联结关系的研究

    欧祖兰; 李先琨; 苏宗明; 唐润琴; 宁世江; 李瑞棠


    Different indices were used based on a 2×2 contingency table to determine the interconnection of the 15 main tree populations of Abies yuanbaoshanensis community in 25m2,50m2 and 100m2 size quadrates.The results showed that the quadrates of 100m2 could exactly display the character of connection between main tree populations and whole community.There was positive correlation among main tree populations in general.But most pairs of main tree populations displayed either positive or negative correlation.A few pairs displayed significant or more correlation.The interconnection of main tree populations was associated with the ecological character of the species.

  6. Morphometric traits of Picea abies L. and Pinus sylvestris L. on the copper dump-field Maximilián in Špania Dolina (Starohorské vrchy Mts., Slovakia

    Tomáš Štrba


    Full Text Available The main aim of the work was to determine difference in length of needles and annual wood increments of Pinus sylvestris L. and Picea abies L. on the dump-field and for comparison on referential site. We performed 500 measurements of needles for each tree species and for each study area. The annual wood increments were measured on wood bores from five tree individuals from each species and from both study areas – dump-field and referential site. Student t-test and indicators of central values evaluated differences of the measured attributes. The results show statistically significant difference at 99% level of confidence for both attributes – length of needles and annual wood increments. The results support the hypothesis about influence of heavy metals on the plants growing.

  7. Comparative study on the turnover of quinic- and shikimic acid and of its derivatives in needles of Norway spruce, Picea abies (L. ) Karst. , affected by 'Waldsterben' syndrome

    Dittrich, P.; Senser, M.; Frielinghaus, J.


    A comparative study on the incorporation of /sup 14/C into quinic- and shikimic acid in spruce needles was carried out with four different syndroms of Picea abies (L.) Karst: a tree from high altitudes of the Bayerischer Wald with the syndrom of 'acute yellowing', a light-sensitive mutant with yellowish needles (Picea abies Karst. finedonensis), a tree exhibiting 'Lametta' syndrome with a translucent crown and twigs hanging down like tinsel, and a healthy tree for control. The needles of these trees were labelled via photosynthetic fixation of /sup 14/CO/sub 2/ in June, and the fate of labelled compounds was monitored over 125 days. In the youngest needles incorporation rates of quinic and shikimic acid reached a dominating level of about 60% of the label of soluble metabolites, which underlines the decisive role of both acids during needle development. The yellow mutant 'finedonensis' and the tree with 'Lametta' syndrome, which exhibit rather identical turnover kinetics of quinate and shikimate, show lower rates and reach, in particular in one- and two-year-old needles, only about 50% of the incorporation rates of the control spruce. The tree with the syndrome of 'acute yellowing' exhibits a significant reduction of incorporation already in the youngest needles; the older needles of this tree virtually suspend metabolism of both acids all together, though apparently high but dormant pools of shikimate and quinate are present. The degree of label incorporation into shikimate and quinate may possibly serve as a measure of needle damage. Exclusively in the yellow mutant a novel spruce constituent, 3-0-p-coumaroylquinic acid, could be detected; a related compound, 3-0-p-caffeoylshikimic acid was identified in the needles of the 'acute yellowing' tree.

  8. Paramètres de croissance et d'exploitation de la carpe blanche Pomadasys jubelini (Cuvier, 1830 dans les complexes lagunaires de Grand-Lahou, Ebrié et Aby (Côte d'Ivoire, Afrique de l'Ouest

    Bodji Iridjé, M.


    Full Text Available Growth and exploitation parameters of the Sompat grunt Pomadasys jubelini (Cuvier, 1830 in Grand-Lahou, Ebrié and Aby lagoons complex (Ivory Coast, West Africa. The growth and exploitation parameters of Pomadasys jubelini (Cuvier, 1830 were studied from January 2007 to December 2008 in Grand-Lahou, Ebrié and Aby lagoons. A total of 2284 specimens (8.50 to 32.70 cm FL were examined. Length-frequency data collected were analyzed with FiSAT software (FAO-ICLARM Fish Stock Assessment Tool. The demographic structure of P. jubelini was composed of young individuals with a majorant allometric growth. Estimation of growth and exploitation parameters gave: L∞= 33.60 cm; K= 0.53 year-1; t0= - 0,30 year; φ'= 2.78; M= 1.10 year-1; F= 0.26 year-1; E= 0.19 in Grand-Lahou lagoon, L∞= 31.50 cm; K= 0.56 year-1; t0= - 0,29 year; φ'= 2.75; M= 1.21 year-1; F= 0.63 year-1; E= 0.34 in Ebrié lagoon and L∞= 31.50 cm; K= 0.57 year-1; t0= - 0,28 year; φ'= 2.74; M= 1.22 year-1; F= 0.71 year-1; E= 0.37 in Aby lagoon. Pomadasys jubelini stock was more exploited in lagoon Aby than in the other lagoons. The maximum recruitment was observed from July in Grand-Lahou and Ebrié lagoons and May in Aby lagoon.

  9. Biotic and abiotic factors associated with soil suppressiveness to Rhizoctonia solani Fatores bióticos e abióticos associados à supressividade de solos a Rhizoctonia solani

    Raquel Ghini


    s propriedades abióticas (pH, condutividade elétrica, teor de matéria orgânica, N total, P, K, Ca, Mg, Al, H, S, Na, Fe, Mn, Cu, Zn, B, capacidade de troca catiônica, soma de bases e saturação de bases e bióticas (atividade microbiana do solo, avaliada pelo desprendimento de CO2 e hidrólise de diacetato de fluoresceína; comunidades de bactérias, fungos, actinomicetos, protozoários, Pseudomonas fluorescentes e Fusarium spp.. A contribuição e relação dessas variáveis para a supressividade a R. solani foram quantificadas por análise de coeficientes de trilha. Quando se avaliaram todas as amostras em conjunto, somente variáveis abióticas apresentaram correlação com a supressão a R. solani, mas o conjunto das variáveis explicou somente 51% da variação total. Entretanto, quando as amostras foram agrupadas e analisadas considerando o tipo de cobertura vegetal, o conjunto de variáveis explicou mais de 90% da variação da supressividade. Para as áreas de floresta e pasto/pousio, as quais foram classificadas como as mais supressivas, algumas variáveis abióticas e a hidrólise de diacetato de fluoresceína apresentaram correlação com a supressão de R. solani e o conjunto de variáveis explicou mais de 98% da supressividade nesses solos.

  10. AtMyb7, a subgroup 4 R2R3 Myb, negatively regulates ABA-induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5

    Kim, Junhyeok


    Various Myb proteins have been shown to play crucial roles in plants, including primary and secondary metabolism, determination of cell fate and identity, regulation of development and involvement in responses to biotic and abiotic stresses. The 126 R2R3 Myb proteins (with two Myb repeats) have been found in Arabidopsis; however, the functions of most of these proteins remain to be fully elucidated. In the present study, we characterized the function of AtMyb7 using molecular biological and genetic analyses. We used qRT-PCR to determine the levels of stress-response gene transcripts in wild-type and atmyb7 plants. We showed that ArabidopsisAtMyb7 plays a critical role in seed germination. Under abscisic acid (ABA) and high-salt stress conditions, atmyb7 plants showed a lower germination rate than did wild-type plants. Furthermore, AtMyb7 promoter:GUS seeds exhibited different expression patterns in response to variations in the seed imbibition period. AtMyb7 negatively controls the expression of the gene encoding bZIP transcription factor, ABI5, which is a key transcription factor in ABA signalling and serves as a crucial regulator of germination inhibition in Arabidopsis. © 2014 John Wiley & Sons Ltd.

  11. Seasonal dynamics in the stable carbon isotope composition δ¹³C from non-leafy branch, trunk and coarse root CO₂ efflux of adult deciduous (Fagus sylvatica) and evergreen (Picea abies) trees.

    Kuptz, Daniel; Matyssek, Rainer; Grams, Thorsten E E


    Respiration is a substantial driver of carbon (C) flux in forest ecosystems and stable C isotopes provide an excellent tool for its investigation. We studied seasonal dynamics in δ¹³C of CO₂ efflux (δ¹³C(E)) from non-leafy branches, upper and lower trunks and coarse roots of adult trees, comparing deciduous Fagus sylvatica (European beech) with evergreen Picea abies (Norway spruce). In both species, we observed strong and similar seasonal dynamics in the δ¹³C(E) of above-ground plant components, whereas δ¹³C(E) of coarse roots was rather stable. During summer, δ¹³C(E) of trunks was about -28.2‰ (Beech) and -26.8‰ (Spruce). During winter dormancy, δ¹³C(E) increased by 5.6-9.1‰. The observed dynamics are likely related to a switch from growth to starch accumulation during fall and remobilization of starch, low TCA cycle activity and accumulation of malate by PEPc during winter. The seasonal δ¹³C(E) pattern of branches of Beech and upper trunks of Spruce was less variable, probably because these organs were additionally supplied by winter photosynthesis. In view of our results and pervious studies, we conclude that the pronounced increases in δ¹³C(E) of trunks during the winter results from interrupted access to recent photosynthates.

  12. Seasonal patterns of carbon allocation to respiratory pools in 60-yr-old deciduous (Fagus sylvatica) and evergreen (Picea abies) trees assessed via whole-tree stable carbon isotope labeling.

    Kuptz, Daniel; Fleischmann, Frank; Matyssek, Rainer; Grams, Thorsten E E


    • The CO(2) efflux of adult trees is supplied by recent photosynthates and carbon (C) stores. The extent to which these C pools contribute to growth and maintenance respiration (R(G) and R(M), respectively) remains obscure. • Recent photosynthates of adult beech (Fagus sylvatica) and spruce (Picea abies) trees were labeled by exposing whole-tree canopies to (13) C-depleted CO(2). Label was applied three times during the year (in spring, early summer and late summer) and changes in the stable C isotope composition (δ(13) C) of trunk and coarse-root CO(2) efflux were quantified. • Seasonal patterns in C translocation rate (CTR) and fractional contribution of label to CO(2) efflux (F(Label-Max)) were found. CTR was fastest during early summer. In beech, F(Label-Max) was lowest in spring and peaked in trunks during late summer (0.6 ± 0.1, mean ± SE), whereas no trend was observed in coarse roots. No seasonal dynamics in F(Label-Max) were found in spruce. • During spring, the R(G) of beech trunks was largely supplied by C stores. Recent photosynthates supplied growth in early summer and refilled C stores in late summer. In spruce, CO(2) efflux was constantly supplied by a mixture of stored (c. 75%) and recent (c. 25%) C. The hypothesis that R(G) is exclusively supplied by recent photosynthates was rejected for both species.

  13. Embyo-Specific Expression of Tomato S/AB/3 Results in Inhibition of Preharvest Sprouting in G46B of Rice%种胚特异性表达番茄SlABI3对水稻冈46B穗萌的抗性研究

    肖英勇; 高永峰; 唐维; 余进德; 刘亮亮; 张霞; 孟蒙; 刘永胜


    [目的]利用水稻胚特异表达基因OsVP1的启动子驱动与OsVP1同源的番茄SlABI3在穗萌严重的水稻保持系冈46B中表达,以增强冈46B对穗萌的抗性,进而为改良水稻穗萌抗性的分子设计育种提供理论依据.[方法]构建SlABI3的胚特异表达载体pHB-0sVP1P::SlABI3,利用根癌农杆菌介导的遗传转化法将其导入籼稻冈46B中,通过研究转基因和野生型植株的穗萌差异,验证种胚特异表达的SlABI3在穗萌抑制中的生理功能.[结果]获得了pHB-0sVP1P::SlABI3转冈46B的转基因植株;转基因植株种子的萌发速率较野生型冈46B慢,并且萌发后第8天转基因植株的苗长、根长显著(P<0.05)短于野生型冈46B;此外,在不同浓度ABA处理的试验中,随着ABA浓度的升高,转基因和野生型种子的萌发速率都呈现下降的趋势,但转基因种子的萌发速率均比野生型种子慢.[结论]SlABI3在水稻胚中特异表达能够降低种子的萌发速率并且抑制穗萌发生.%[Objective] In order to increase resistance to preharvest sprouting in maintain line G46B of rice, the promoter of rice OsVP1 was used to drive tomato SIABI3 (homologous to OsVP1) expression in rice embryos. This will probably provide a theoretical basis of molecular design for improving preharvest sprouting resistance in hybrid rice.[Method]The expression vector containing the full-length coding region of SlABI3 whose expression is driven by the OsVPl promoter was constructed and introduced into rice G46B by Agrobacterium-mediated genetic transformation. To further elucidate the physiological function of SIABI3 in inhibiting the pre-harvest sprouting, the phenotypic differences in germination between transgenic and wild-type seeds were observed. [Result] A number of transgenic plants containing pHB-OsVPlP::SLABI3 were obtained. Germination experiments showed that the percentage of geminated seeds from transgenic plants was lower than that of wild-type G46B plants

  14. Indução de resistência do mamoeiro à podridão radicular por indutores bióticos e abióticos Resistance induction to root rot in papaya by biotic and abiotic elicitors

    Giltembergue Macedo Tavares


    Full Text Available O objetivo deste trabalho foi avaliar o potencial do uso de indutores de resistência bióticos e abióticos na redução da podridão radicular em mamoeiro. Mudas de mamoeiro foram pulverizadas com os fungicidas fosetil-Al, metalaxil e Mancozeb (2 g L-1, com os indutores abióticos fosfito de potássio (2,5 e 5 mL L-1, ácido salicílico 0,15 e 0,30%, Reforce (indutor comercial + ácido salicílico a 5%, acibenzolar-S-metil (ASM (0,15 e 0,30 g L-1, e com o indutor biótico Saccharomyces cerevisiae (3 e 6 mL L-1, três e seis dias antes da pulverização de 1 mL de suspensão de 10(5 zoósporos mL-1 de Phytophthora palmivora. Todos os tratamentos tiveram efeito no controle da podridão de raízes em relação à testemunha, com exceção do Reforce + ácido salicílico a 5% (3 mL L-1, seis dias antes da inoculação. Os tratamentos com ASM, com exceção da dosagem 0,15 g L-1 seis dias antes da inoculação, apresentaram resultados similares aos dos fungicidas metalaxil e Mancozeb. Plantas pulverizadas com ASM apresentaram aumento de atividade da peroxidase e beta-1,3-glucanase e maior concentração de lignina que a testemunha. No entanto, esses tratamentos não tiverem efeito sobre a atividade da quitinase. O ASM é um potencial indutor de resistência a P. palmivora em mamoeiro.The objective of this work was to evaluate the potential use of biotic and abiotic elicitors of resistance in the reduction of root rot in papaya. Papaya seedlings were sprayed with the fosetil-Al and metalaxyl and Mancozeb (2 g L-1 fungicides, with the potassium phosphite (2.5 and 5 mL L-1, salicylic acid 0.15 e 0.30%, Reforce (commercial product + salicylic acid 5%, acibenzolar-S-methyl (ASM (0.15 and 0.30 g L-1 abiotic elicitors, and with the Saccharomyces cerevisiae (3 and 6 mL L-1 biotic elicitor, applied three and six days before the inoculation with 1 mL of 10(5 zoospores mL-1 suspension of Phytophthora palmivora. All treatments were effective in controlling

  15. Community Structure and Species Diversity of Abies georgei var.smithii Forest near the Timerline%急尖长苞冷杉林林线附近群落结构与物种多样性

    张晓娟; 罗大庆


    急尖长苞冷杉(Abies georgei var.smithii)是西藏东南部高山林线区森林群落的主要建群树种,分布于色季拉山海拔3 600~4 400 m区域,为阴坡高山林线的优势树种.在藏东南色季拉山东坡急尖长苞冷杉林林线附近海拔4 000~4 400m地带进行样地样方调查的基础上,分析急尖长苞冷杉群落的物种组成、物种丰富度(S)、Simpson指数(D)、Shannon-Wiener指数(H')、均匀度指数(Ea)、Jaccard指数(Cj)、Cody指数(βc)以及种群结构与生长特征.结果表明:1)在林线5个海拔的调查样地中,共有种子植物26种,分属16科20属;随海拔梯度的升高,植物的科、属、种的数量总体呈减少趋势,灌木层和草本层的丰富度亦呈相似的趋势;2)林线附近的灌木层和草本层植物的α多样性与海拔总体呈负相关;3)在生境转折带的4 100~4 200 m之间Jaccard指数(Cj)最低,Cody指数与海拔呈负相关;4)林线附近的急尖长苞冷杉种群总体呈稳定的金字塔型结构;5)在林线附近,海拔4 200 m的急尖长苞冷杉的分布与生长状况相对较优.%Abies georgei var. Smithii is a main constructive species of the forest communities at alpine timer-line in southeast area of Tibet. It is also the dominant tree species on the shady slope in this area,and distributed between 3 600 to 4 400 m above sea level at the Mt. Sejila. Based on a comprehensive investigation on A. Georgei population that distributed between the altitudes of 4 000 to 4 400 m,an analysis was carried out on the species composition, species richness, Simpson index, Shannon-Wiener index, Evenness index, Jaccard index,Cody index, changes in the structure, and growth characteristics. The results were reported as follows. 1) There were 26 species of wild seed plants belonging to 16 families and 20 genera in sampling plots. The number of families,genera and species decreased with the increase of altitude. Species richness of shrubs and herbs also

  16. Sensitivity of multiplex real-time PCR reactions, using the LightCycler and the ABI PRISM 7700 Sequence Detection System, is dependent on the concentration of the DNA polymerase.

    Exner, M M; Lewinski, M A


    The introduction of multiplex PCR techniques to clinical laboratories has provided a means to streamline assays and to produce multiple results with minimal effort. While this methodology is very beneficial, care must be taken to ensure that reactions are properly optimized to allow for maximum sensitivity. This study was conducted to determine whether the sensitivity of multiplex-real-time PCR assays could be improved by increasing the concentration of DNA polymerase within a reaction. Multiplex reactions were designed to simultaneously detect the human HLA-DQ gene and a sequence from the UL83 region of the CMV genome. Two real-time PCR systems, one utilizing AmpliTaq Gold DNA polymerase and the ABI 7700 Sequence Detection System, and one utilizing FastStart Taq DNA polymerase and the Roche LightCycler were tested. The results indicated that increasing the AmpliTaq Gold concentration from 0.050 to 0.10 U/microl and the FastStart Taq concentration from 0.1875 to 0.375 U/microl increased detection sensitivity from 5,000 to 50 CMV copies per PCR reaction. In separate experiments, commercially prepared mastermixes were utilized for both real-time PCR platforms as per the manufacturer's suggestions or with the addition of supplemental DNA polymerase. In assays designed to detect 4 CMV genome copies per reaction, the addition of 2.5 U of AmpliTaq Gold to TaqMan Universal Mastermix increased the detection rate from 21 to 67%, and the addition of 5 U of FastStart Taq to FastStart DNA Master Hybridization Probes mastermix increased the detection rate from 17 to 56%. These results indicate that increasing the DNA polymerase concentration in multiplex real-time PCR reactions may be a simple way to optimize assay sensitivity.

  17. Evaluation of the RapidHIT™ 200 System: A comparative study of its performance with Maxwell(®) DNA IQ™/Identifiler(®) Plus/ABI 3500xL workflow.

    Thong, Zhonghui; Phua, Yong Han; Loo, Eileen Shuzhen; Goh, Sze Kae; Ang, Jiatian; Looi, Woan Foon; Syn, Christopher Kiu Choong


    RapidHIT(™) System is a rapid DNA instrument that is capable of processing forensic samples from extraction through to capillary electrophoresis and profile generation within two hours. Evaluation of the RapidHIT(™) 200 System was conducted to examine several key performance indicators of the instrument, including reproducibility, contamination, sensitivity, versatility and the possibility of sample re-extraction. Results indicated that the RapidHIT(™) 200 System was capable of generating high quality DNA profiles which were comparable to those from the standard protocol comprising of Maxwell(®) 16 DNA IQ(™) System, Identifiler(®) Plus and ABI 3500xL. No contamination was detected during the studies. Results also showed that the instrument was able to generate DNA profiles from samples containing lower amounts of DNA (0.5 μl of blood) albeit with more allele and locus dropouts when compared to the standard protocol. The ability to process blood swabs, blood-stained FTA punches, semen swabs, buccal swabs, product of conception (POC), bone marrow, fingernail clippings and cigarette butts at a good success rate indicated the robustness and versatility of the RapidHIT(™) 200 System. Furthermore, additional alleles could be recovered via re-analysis of the failed samples using the standard protocol. In summary, our results showed that the RapidHIT(™) 200 System was able to process casework samples for the purpose of providing rapid intelligence through DNA database searches and reference matching. Confirmative DNA results can be obtained through either concurrent processing of duplicate samples via standard protocol or re-extraction of samples retrieved from the RapidHIT(™) sample cartridge.

  18. Identification and quantitative determination of pinoresinol in Taxus ×media Rehder needles, cell suspension and shoot cultures

    Paulina Mistrzak


    Full Text Available The aim of our study was to investigate the presence and quantitative contents of lignans in the tissues of Taxus ×media. The presence of the lignans: pinoresinol, matairesinol and secoisolariciresinol was assessed in needles, shoots cultures and suspension culture. Pinoresinol was the only lignan found in the tissue of T. ×media. The total pinoresinol content in the needles and in the shoots was 1.24 mg/g dry weight (dw and 0.69 mg/g dw, respectively. Most of the pinoresinol identified was appeared glycosidically bound. In needles, the amount of glycosidically bound pinoresinol (0.81 mg/g dw was about twice as high as that of free pinoresinol (0.43 mg/g dw. The content of free and glycosidically bound pinoresinol showed the level of 0.18 mg/g dw and 0.51 mg/g dw, respectively in the in vitro shoot cultures. In the cell culture, no pinoresinol was found.

  19. Clinical Study of the Value of PCT, ABI and 128 Hz Tuning Fork Combined with 10 g Monofilament in Evaluating the Prognosis of Diabetic Foot%PCT、 ABI、128 Hz音叉联合10克单丝对糖尿病足预后评估价值

    刘强; 游东; 梁永


    目的:探讨降钙素原(PCT)、踝肱指数(ABI)、 Rydel-seifer分级音叉(128 Hz音叉)联合10 g单丝对糖尿病足预后评估的临床价值。方法选择243例糖尿病患者,其中观察组为糖尿病足患者50例,对照组为非糖尿病足183例,对其临床资料进行回顾性分析糖尿病足的相关因素。根据患者预后分为两组,比较其PCT水平、 ABI、128 Hz音叉联合10 g单丝结果。结果多因素 Logistic 回归分析显示,肥胖、空腹血糖、128 Hz音叉及PCT升高、 ABI、10 g单丝降低是糖尿病足的危险因素。截肢组患者PCT水平及128 Hz音叉结果高于非截肢组, ABI、10 g单丝结果低于非截肢组,两组比较,差异具有统计学意义, P<0.05。结论肥胖、空腹血糖、128 Hz音叉及PCT升高、 ABI、10 g单丝降低在糖尿病的进展过程中起着重要作用,应给予足够重视,积极对症治疗,降低其截肢率。%Objective To investigate the clinical value of procalcitonin (PCT), ankle brachial index (ABI) and Rydel-seifer tuning fork rating (128 Hz tuning fork) combined with 10 g monofilament in evaluating the prognosis of diabetic foot. Methods 243 cases of patients with diabetes were selected, the observation group of 50 patients with diabetic foot, compared with the control group of 183 cases of non diabetic foot. The clinical data were retrospectively analyzed and the related factors of diabetic foot were analyzed by SPSS19. 0 statistics software. The patients were divided into 2 groups according to the prognosis of the patients and the results of the level of PCT, ABI, and 128 Hz fork combined with 10g monofilament. Results Multivariate logistic regression analysis showed that the increase of obesity, fasting blood glucose, 128 Hz tuning fork and PCT and the decrease of ABI and 10 g monofilament were the risk factors for diabetic foot. The scores of the level of PCT level and 128 Hz tuning fork of the patients in amputation group

  20. Influência dos fatores abióticos e da disponibilidade de presas sobre comunidade de serpentes do Planalto Médio do Rio Grande do Sul Influence of abiotic factors and availability of prey on the occurrence of snakes in the middle plateau region of Rio Grande do Sul, Brazil

    Noeli Zanella; Sonia Z. Cechin


    A influência dos fatores abióticos sobre a disponibilidade de presas e a dieta das espécies de serpentes mais abundantes do Planalto Médio do Rio Grande do Sul, foi estudada em duas áreas: floresta e campo. O trabalho foi desenvolvido utilizando serpentes coletadas com os métodos: procura limitada por tempo, encontros ocasionais, armadilhas de interceptação e queda, e serpentes depositadas na coleção de répteis da Universidade de Passo Fundo. Foram registradas as guildas alimentares das seis ...

  1. Fatores abióticos definidores da distribuição dos diferentes tipos florestais (floresta paludícula, floresta estacional semidecídua ribeirinha e cerradão), nos municípios de Batatais e Restinga, SP


    Com o objetivo de compreender quais fatores abióticos determinam a ocorrência das diferentes formações florestais na região de Batatais, entre as coordenadas 20038S e 20058S e 47037W e 47028W, foram escolhidas três propriedades agrícolas (Fazenda Monte Belo, em Restinga, SP e Fazenda Magnólia e Sítio Pratinha, em Batatais, SP), que apresentavam remanescentes florestais representativos da região (floresta ribeirinha paludícula, floresta estacional semidecídua ribeirinha e cerradão). Para descr...

  2. 应用GIS和最大熵模型分析秦岭冷杉潜在地理分布%Using GIS and MaxEnt to analyze the potential distribution of Abies chensiensis

    高蓓; 卫海燕; 郭彦龙; 顾蔚


    秦岭冷杉(Abies chensiensis)为松科常绿针叶乔木,属国家濒危野生物种.本文利用获取的秦岭冷杉地理分布记录和14个气候指标、3个土壤指标和3个地形指标,通过最大熵模型(MaxEnt)和ArcGIS空间分析构建秦岭冷杉潜在分布区.结果表明,影响秦岭冷杉潜在生境适宜性的主要环境指标为6个气候指标(年平均气温、年极端最高气温、1月平均气温、≥0℃积温、年干燥度指数和年日照时数)、1个土壤指标(pH)和1个地形指标(海拔).研究区秦岭冷杉生长的最适宜区面积为19498.87 km2,适宜区面积为32219.61 km2,边缘适宜区面积为51874.76 km2,不适宜区面积为106307.97 km2,秦岭冷杉适宜生长区域狭窄,其中最适宜区和适宜区主要集中在甘肃东南部、陕西中南部、四川东北部,湖北西北部和河南西北部,适宜区内次生植被丰富、地形复杂.研究表明,基于最大熵模型与GIS空间分析构建的秦岭冷杉潜在地理分布,可以对我国秦岭冷杉适宜生长区做出科学的区划,为秦岭冷杉资源的保护和管理提供科学依据.

  3. Non-functional plastid ndh gene fragments are present in the nuclear genome of Norway spruce (Picea abies L. Karsch): insights from in silico analysis of nuclear and organellar genomes.

    Ranade, Sonali Sachin; García-Gil, María Rosario; Rosselló, Josep A


    Many genes have been lost from the prokaryote plastidial genome during the early events of endosymbiosis in eukaryotes. Some of them were definitively lost, but others were relocated and functionally integrated to the host nuclear genomes through serial events of gene transfer during plant evolution. In gymnosperms, plastid genome sequencing has revealed the loss of ndh genes from several species of Gnetales and Pinaceae, including Norway spruce (Picea abies). This study aims to trace the ndh genes in the nuclear and organellar Norway spruce genomes. The plastid genomes of higher plants contain 11 ndh genes which are homologues of mitochondrial genes encoding subunits of the proton-pumping NADH-dehydrogenase (nicotinamide adenine dinucleotide dehydrogenase) or complex I (electron transport chain). Ndh genes encode 11 NDH polypeptides forming the Ndh complex (analogous to complex I) which seems to be primarily involved in chloro-respiration processes. We considered ndh genes from the plastidial genome of four gymnosperms (Cryptomeria japonica, Cycas revoluta, Ginkgo biloba, Podocarpus totara) and a single angiosperm species (Arabidopsis thaliana) to trace putative homologs in the nuclear and organellar Norway spruce genomes using tBLASTn to assess the evolutionary fate of ndh genes in Norway spruce and to address their genomic location(s), structure, integrity and functionality. The results obtained from tBLASTn were subsequently analyzed by performing homology search for finding ndh specific conserved domains using conserved domain search. We report the presence of non-functional plastid ndh gene fragments, excepting ndhE and ndhG genes, in the nuclear genome of Norway spruce. Regulatory transcriptional elements like promoters, TATA boxes and enhancers were detected in the upstream regions of some ndh fragments. We also found transposable elements in the flanking regions of few ndh fragments suggesting nuclear rearrangements in those regions. These evidences

  4. Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.

    Bohlmann, J; Steele, C L; Croteau, R


    Grand fir (Abies grandis) has been developed as a model system for studying defensive oleoresin formation in conifers in response to insect attack or other injury. The turpentine fraction of the oleoresin is a complex mixture of monoterpene (C10) olefins in which (-)-limonene and (-)-alpha- and (-)-beta-pinene are prominent components; (-)-limonene and (-)-pinene synthase activities are also induced upon stem wounding. A similarity based cloning strategy yielded three new cDNA species from a wounded stem cDNA library that appeared to encode three distinct monoterpene synthases. After expression in Escherichia coli and enzyme assay with geranyl diphosphate as substrate, subsequent analysis of the terpene products by chiral phase gas chromatography and mass spectrometry showed that these sequences encoded a (-)-limonene synthase, a myrcene synthase, and a (-)-pinene synthase that produces both alpha-pinene and beta-pinene. In properties and reaction stereochemistry, the recombinant enzymes resemble the corresponding native monoterpene synthases of wound-induced grand fir stem. The deduced amino acid sequences indicated the limonene synthase to be 637 residues in length (73.5 kDa), the myrcene synthase to be 627 residues in length (72.5 kDa), and the pinene synthase to be 628 residues in length (71.5 kDa); all of these monoterpene synthases appear to be translated as preproteins bearing an amino-terminal plastid targeting sequence. Sequence comparison revealed that these monoterpene synthases from grand fir resemble sesquiterpene (C15) synthases and diterpene (C20) synthases from conifers more closely than other monoterpene synthases from angiosperm species. This similarity between extant monoterpene, sesquiterpene, and diterpene synthases of gymnosperms is surprising since functional diversification of this enzyme class is assumed to have occurred over 300 million years ago. Wound-induced accumulation of transcripts for monoterpene synthases was demonstrated by RNA

  5. Height Extraction and Stand Volume Estimation Based on Fusion Airborne LiDAR Data and Terrestrial Measurements for a Norway Spruce [Picea abies (L. Karst.] Test Site in Romania

    Bogdan APOSTOL


    Full Text Available The objective of this study was to analyze the efficiency of individual tree identification and stand volume estimation from LiDAR data. The study was located in Norway spruce [Picea abies (L. Karst.] stands in southwestern Romania and linked airborne laser scanning (ALS with terrestrial measurements through empirical modelling. The proposed method uses the Canopy Maxima algorithm for individual tree detection together with biometric field measurements and individual trees positioning. Field data was collected using Field-Map real-time GIS-laser equipment, a high-accuracy GNSS receiver and a Vertex IV ultrasound inclinometer. ALS data were collected using a Riegl LMS-Q560 instrument and processed using LP360 and Fusion software to extract digital terrain, surface and canopy height models. For the estimation of tree heights, number of trees and tree crown widths from the ALS data, the Canopy Maxima algorithm was used together with local regression equations relating field-measured tree heights and crown widths at each plot. When compared to LiDAR detected trees, about 40-61% of the field-measured trees were correctly identified. Such trees represented, in general, predominant, dominant and co-dominant trees from the upper canopy. However, it should be noted that the volume of the correctly identified trees represented 60-78% of the total plot volume. The estimation of stand volume using the LiDAR data was achieved by empirical modelling, taking into account the individual tree heights (as identified from the ALS data and the corresponding ground reference stem volume. The root mean square error (RMSE between the individual tree heights measured in the field and the corresponding heights identified in the ALS data was 1.7-2.2 meters. Comparing the ground reference estimated stem volume (at trees level with the corresponding ALS estimated tree stem volume, an RMSE of 0.5-0.7 m3 was achieved. The RMSE was slightly lower when comparing the ground

  6. Britid suurendasid ELi uusliikmete abi / Marit Ruuda

    Ruuda, Marit


    Saksamaa ja Prantsusmaa moodustasid Euroopa Liidu eelarvekava läbirääkimistel ühisrinde ning nõudsid brittidelt nende tagasimakse kadumist aastaks 2013, aga ka ELi uusliikmete abiraha suurendamist

  7. Firmad loodavad abi saada sundpuhkustest / Valdu Toomla

    Toomla, Valdu


    Teisel poolaastal on esitanud ning tööinspektsioonilt loa saanud 11 Lääne-Virumaa ettevõtet kokku 222 töötajaga ja menetlemist ootab veel 6 ettevõtet, kes soovisid saata ühtekokku 180 töötajat osaliselt tasustatavale puhkusele või viia osalisele tööajale

  8. Vananemise vastu otsitakse abi noorte verest


    Füüsik Jesse Karmazin koos endise vaktsiiniuurija Craig Wrightiga panid aluse uuringule (katses osaleks 600 vähemalt 35-aastast inimest, kellele kantakse üle kuni 25-aastaste verest pärit plasma), et uurida vereplasma kui võimaliku noorendava eliksiiri toimet. Kommenteerinud Toivo Maimets

  9. Abi Ida-Euroopale / Sirje Rank

    Rank, Sirje, 1966-


    Ida-Euroopast on saamas riskikoht ning koos nende riikide langusega kannataks ka laenuandja Lääne-Euroopa. Suurbritannia peaminister ja Prantsusmaa president arutasid ka Ida-Euroopa abistamist. Lisa: EL plaanib kahekordistada abilaenude mahtu

  10. Omavalitsused ootavad eelarvest abi / Toomas Varek

    Varek, Toomas, 1948-


    Ilmunud ka: Valgamaalane, 2.okt. 2007, lk. 2; Meie Maa, 27. sept. 2007, lk. 2; Vali Uudised, 28. sept. 2007, lk. 2; Sakala, 28. sept 2007, lk. 2; Harju Ekspress, 28. sept. 2007, lk. 4; Koit, 29. sept. 2007, lk. 6; Vooremaa, 29. sept. 2007, lk. 2; Lõunaleht, 4. okt. 2007, lk. 2. Riigieelarve kontrolli komisjoni esimehe hinnangul pole koalitsioonilepingus kohaliku omavalitsuse tulubaasi süsteemselt käsitletud ning valitsuse positsioon ja eelarveläbirääkimiste senine käik ei anna lootust kiireteks lahendusteks

  11. Tudengid otsivad abi õiguskantslerilt / Dannar Leitmaa

    Leitmaa, Dannar, 1982-


    Õppemaksu tõstmisest Tallinna ja Tartu Ülikoolis ja ülikooliseaduse vastava regulatsiooni tõlgendamisest ning üliõpilaste märgukirjast õiguskantslerile. Lisatud advokaat Ramon Raski kommentaar. Seisukoha avaldavad ka Tallinna Ülikooli akadeemiline prorektor Heli Mattisen, Tartu Ülikooli prorektor Birute Klaas ja Eesti Üliõpilaskondade Liidu juhatuse esimees Eimar Veldre

  12. Vennalik abi / Raul-Levroit Kivi

    Kivi, Raul-Levroit, 1920-2009


    Vabariikliku projekteerimisinstituudi "Estonprojekt" Tartu filiaali juhataja Kuno Tiku väljavahetamisest Venemaalt tulnud Nikolai Toomega. Arhitektide vastuhakust. Lk. 83-84 1954. a. 24. detsembri "Edasis" pseudonüümi E. Saar all ilmunud Olaf Uti kollektiivi toetav artikkel "Ühe kollektiivi mured"

  13. ‘”Cannon fodder for respectable question marks”: Fritz Saxl and the history of the Warburg Library’. Review of: Dorothea McEwan, Fritz Saxl – Eine Biografie: Aby Warburgs Bibliothekar und Erster Direktor des Londoner Warburg Institutes, Wien, Köln, Weimar: Böhlau Verlag, 2012

    Mark A. Russell


    Full Text Available As the first Archivist of The Warburg Institute, University of London, from 1993 until her retirement in 2006, Dorothea McEwan compiled the database of the Aby Warburg Correspondence. McEwan has published and lectured widely on Warburg and Fritz Saxl. The present book is preceded by two previous volumes treating the Warburg-Saxl correspondence: Das Ausreiten der Ecken, Hamburg: Dölling und Galitz, 1998; and Wanderstrassen der Kultur, München und Hamburg: Dölling und Galitz, 2004. Based largely on Fritz Saxl’s correspondence in his various capacities as Aby Warburg’s principal aid and successor, this recounting of the Austrian scholar’s life and work is presented as a narration of the events of his professional career, and not as an intellectual biography per se. Saxl is situated within a history of the Warburg Library, is pictured as devoted to Warburg and his work, and is shown to have been critical to the functioning and survival of the Library in its various forms. As such, the book suggests that Saxl’s greatest achievement was his administrative and organizational contribution to what became the Warburg Institute. Surveying and integrating a large body of material, the author provides the necessary outlines of a career and corpus of scholarship worthy of further exploration.

  14. 滇西北不同海拔梯度下急尖长苞冷杉叶片的生态解剖结构特性研究%An Eco-anatomical Study on Abies georgei var. smithii Leaves at Gradient Elevation in Northwest of Yunnan

    唐探; 姜永雷; 冯程程; 程小毛; 黄晓霞


    采用生态解剖学的方法对玉龙雪山自然保护区内不同海拔梯度(3100 m,3250 m,3400 m,3550 m,3700 m)的急尖长苞冷杉叶片解剖结构特征进行比较研究,探究不同海拔高度对急尖长苞冷杉叶片生态适应的影响。结果表明:在海拔低于3550 m时,随着海拔的升高,叶片表皮角质层厚度、上表皮厚度、栅栏组织厚度、海绵组织厚度、栅栏组织细胞长短径、栅栏系数、栅栏细胞系数、韧皮部厚度、木质部厚度及转输组织厚度均呈增大趋势,而海拔高于3550 m时,各项指标及参数有所下降。因此,海拔3550 m附近为玉龙雪山急尖长苞冷杉生长最适宜的环境;不同海拔梯度上复杂的环境条件导致了急尖长苞冷杉叶片解剖结构的差异性,并限制了它的生长和分布。这种差异性是植物在一定程度上适应环境的结果。%To study the ecological adaptation of Abies georgei var.Smithii leaves at different gradient eleva⁃tions,the leaf anatomical characteristics of Abies georgei var. Smithii and their relationship with ecological fac⁃tors were investigated,which are growing at different elevations ( 3 100 m,3 250 m,3 400 m,3 550 m,3 700 m) in the Nature Reserve of Yulong Snow Mountain.The results showed that:at the altitude below 3 550 m,the strata corneum epidermidis thickness,upper epidermis thickness,palisade mesophyll thickness,spongy meso⁃phyll thickness,length and width of palisade cell,palisade tissue ratio,palisade cell factor,phloem thickness, xylem thickness and the transfusion tissue thickness increased with the increasing elevation. However,the pa⁃rameters of leaf anatomical characteristics decreased at the elevation above 3 550 m. Hence, the optimum zone for Abies georgei var.Smithii was nearly at the elevation of around 3 550 m.On one hand,the growth and distribution of Abies georgei var.Smithii are limited by the changes of complex environmental conditions

  15. Freezing damage in early spring induced by simulated global warming:a case study in Abies faxoniana%模拟增温引发的早春冻害:以岷江冷杉为例

    徐振锋; 胡庭兴; 张远彬; 鲜骏仁; 王开运


    Global climate change mainly characterized by global warming had changed and been changing the structure and function of terrestrial ecosystem. The relationships between current vegetations and environments are formed through hundreds of thousands of years′ evolution, thus changes in plant phenology may influence these subtle relationships. In this study, the short-term responses of leaf phenology, freezing damage of Abies faxoniana to simulated global warming were investigated using the open-top chamber (OTC) and transposing of surface soil with vegetation (TSSV) methods. The results were shown as follows: In Muyangchang, mean air temperature in the OTC raised by 2.2 ℃ at 1.0 m above the ground in the OTC during the growing season. Moreover, mean air temperature was 2.5 ℃ lower in timberline ecotone (3200 m) than versus in Muyangchang (2600 m). A. faxoniana seedlings in warming treatments showed earlier bud break, later bud dormancy and longer growing season. Unfortunately, severe freezing damage in current buds was detected in warming treatments. The results obtained in this study indicate that frost damage probably poses strong impact on the alpine species under future warming conditions.%以全球变暖为主要特征的全球气候变化已经并正在改变着陆地生态系统的结构和功能.现存植被与环境间的关系是经过漫长自然选择而形成的,因此植物物候变化可能会影响物种与环境间的相互关系.采用开顶式生长室(Open-top chamber,OTC)和移地试验(transposing of surface soil with vegetation,TSSV)模拟增温的方法,研究了川西亚高山岷江冷杉幼苗物候和冻害对模拟增温的短期响应.结果表明,生长季中OTC内日平均气温较对照增加2.2 ℃,高海拔(3 200 m)比低海拔(2 600 m)日平均温度低2.5 ℃.在两种研究方法下,温度升高都使岷江冷杉芽开放提前,休眠期推迟,生长季延长.温度升高使岷江冷杉幼苗新生芽遭受严重的冻害.

  16. 利用无人机遥感测定岷江冷杉单木树干生物量%Estimation of stem biomass of individual Abies faxoniana through unmanned aerial vehicle remote sensing

    何游云; 张玉波; 李俊清; 王卷乐


    无人机获取的高分辨率遥感影像,已成为单木生物量估算的有效手段。本文以四川省王朗自然保护区岷江冷杉为研究对象,利用本项目组自行研制的无人机获取无阴影遥感影像。设置2块样地,其中plot 1为建模样本, plot 2为验证样本。通过人机交互的方式提取单木树冠面积( CA)数据,并结合野外实测的胸径( DBH),建立DBH遥感估算模型。最后基于CA-DBH模型的有效性,结合已有DBH-SB(树干生物量)经验方程,计算plot 2岷江冷杉单木树干生物量。结果表明:基于无人机遥感影像提取的单木CA与实测DBH存在较好的非线性相关关系,所建立的模型有较好的拟合度,R2达到0.752(P0.05),其皮尔森相关系数可达0.879,证明利用无人机获取的遥感影像,通过提取的CA估算DBH是可行的。本次实验表明:利用无人机遥感获取影像,通过提取的单木CA进行树干生物量的估算是有效的。%Fast and accurate quantification of biophysical parameters of trees is essential for forest management, assessment of carbon sequestration and evaluation of regional ecosystem services value. Unmanned aerial vehicle ( UAV ) is a promising tool to estimate biomass of individual trees due to its extremely high resolution. In this study, we used self-developed UAV to obtain shadow-free remote sensing images, taking Abies faxoniana in Wanglang Nature Reserve of Sichuan Province as an example. There were two plots, one for model training and the other for model validation. Crown area (CA) of individual trees was delineated through man-computer interpretation. Meanwhile, the field inventory was conducted to record the diameter at breast height ( DBH) of individual trees, and to establish CA-DBH regression model. Based on the validity of CA-DBH model, the stem biomass ( SB) of individual A. faxoniana trees in plot 2 was derived according to the existing empirical DBH-SB equation. There was a strong nonlinear

  17. Factores bióticos y abióticos que determinan la seroprevalencia de anticuerpos contra Trypanosoma Cruzi en el municipio de Palmar de Bravo, Puebla, México Biotic and abiotic determinants of seroprevalence of antibodies against Trypanosoma cruzi in Palmar de Bravo, Puebla, Mexico

    Francisca Sosa-Jurado


    Full Text Available OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi y su relación con los factores bióticos y abióticos en Palmar de Bravo, Puebla, México. MATERIAL Y MÉTODOS: Estudio transversal efectuado en agosto de 2000 a septiembre de 2001, con una muestra aleatoria simple de 390 voluntarios residentes en Palmar de Bravo, Puebla, México. Se hizo determinación de anticuerpos contra T cruzi con técnicas serológicas validadas, búsqueda del vector y de reservorios domésticos, así como determinación de asociación entre caso positivo y factores de riesgo bióticos y abióticos. El análisis estadístico consistió en índice Kappa para las pruebas diagnósticas, empleando tabla de contingencia de 2 x 2; ji cuadrada corregida de Yates, exacta de Fisher y la razón de posibilidad para estimar la significancia de la asociación de factores bióticos y abióticos. RESULTADOS: La seroprevalencia fue de 4% en la población humana estudiada y de los reservorios (equinos, porcinos y caninos, sólo 10% de los caninos resultaron reactivos. Los vectores identificados fueron T barberi y T pallidipennis, con índice de dispersión e índice de colonización de 55 y 40%, respectivamente. Los factores de riesgo más importantes fueron la altitud (>2 150 y OBJECTIVE: To establish the relationship between seroprevalence for antibodies against Trypanosoma cruzi and its relationship with biotic and abiotic factors. MATERIAL AND METHODS: A cross-sectional study was conducted between August 2000 and September 2001. The study population consisted of a simple random sample of 390 volunteers residing in Palmar de Bravo, Puebla, Mexico. Sample and data collection procedures included assaying antibodies against T cruzi with validated assays, and searching for domestic reservoirs and triatomine bugs. The relationship between biotic and abiotic factors with seropositivity was assessed. Statistical analysis was conducted using Kappa values for

  18. Altitudinal Variation in Aboveground Biomass of Abies georgei var.smithii at Timberline of the Sergyemla Mountains, Southeast Tibet%藏东南色季拉山急尖长苞冷杉林线地带地上生物量随海拔的变化特征

    刘新圣; 张林; 孔高强; 罗天祥


    Environment becomes harsh with increasing altitude, which has major effects on forest biomass accumulation and its allocation to woody and foliage components.In this study, we compared aboveground biomass and its allocation pattern of Abies georgei[var.smithii along a transect from sub-alpine forest to alpine timberline in the Sergyemla Mountain, southeast Tibet.We estimated different components of aboveground biomass for Abies trees and undergrowth Rhododendron shrubs by using allometric regressions.The aboveground biomass of undergrowth herbs was measured by harvesting in 0.5 m ×0.5 m quadrats.Aboveground biomasses were 181 ~284 t/hm2, 117 ~ 248 t/hm2, 35 ~ 62 t/hm2 and 0.9 ~ 1.5 t/hm2 for whole community, trees, undergrowth shrubs and herbs, respectively.Aboveground biomass of the whole community decreased significantly with increasing elevation, and the decreasing rate was 73.1 t/hm2 per 100 m.Furthermore, the Abies forest stand at higher elevation tended to reduce biomass allocation to non-photosynthesis organs (stem and branch), and to increase the allocation to longlived needles.The increased biomass allocation to long-lived needles at higher elevations may extend the mean residence time of nutrients in plants, and then enhance nutrient use efficiency of dry mass production to adapt to the low temperature environment at alpine timberlines.%从亚高山森林到高山林线,逐渐严酷的生长环境影响了生物量的积累和分配.本研究采用相对生长法和样方收获法估算了藏东南色季拉山急尖长苞冷杉林在禾同海拔(4 190 m,4 270 m和4 326 m)的地上生物量,分析了群落中不同层次地上生物量、各器官生物量及其分配比例随海拔的变化格局.结果表明:1.急尖长苞冷杉群落地上生物量在181~284 t/hm2之间,其中,乔木层在11~-248 t/hm2,灌木层为35~62 t/hm2,草本层为0.9~1.5 t/hm2;2.群落地上生物量随海拔升高急剧降低,降低的平均幅度为73.1 t/(hm2

  19. Desenvolvimento de um sistema de apoio à decisão para a diagnose de doenças, pragas e distúrbios abióticos dos citros Development of a decision support system for the diagnosis of citrus diseases, pests, and abiotic factors

    Cleilson do Nascimento Uchôa


    Full Text Available A preocupação com a diagnose dos problemas que afetam as plantas cítricas proporcionou ao longo dos anos o acúmulo de conhecimento, que precisava ser organizado e divulgado de forma eficiente e rápida. Dessa forma, objetivou-se com esse trabalho construir uma base de conhecimentos para diagnóstico de doenças bióticas e abióticas dos citros, desenvolver e implementar o sistema de apoio à decisão (SAD e avaliar o sistema. O SAD foi construído utilizando a ferramenta 'Borland Delphi' versão 5. Foram formuladas 562 perguntas, 322 regras e anexadas fotografias para facilitar o diagnóstico de 34 doenças, 40 pragas e 34 distúrbios abióticos dos citros. A avaliação do SAD foi dividida em verificação e validação. Na verificação, o SAD foi submetido a uma análise por meio de questionário a 5 especialistas em citros. A validação do programa foi realizada com quatro grupos, de diferentes níveis de conhecimento (10 pessoas/grupo, tentando diagnosticar corretamente. Obteve-se um acerto de 45,6% dos usuários e 93,6% do SAD. O sistema foi denominado Dr. Citrus (protótipo com registro no INPI n° 014070009188.The concern about the diagnosis of problems affecting citrus led to an accumulation of knowledge over the years. However, such accumulated knowledge needed to be organized and efficiently disseminated. Thus, the aims of this work were to build a knowledge basis for the diagnosis of abiotic and biotic citrus diseases to develop and implement a Decision Support System (DSS and to test and evaluate this system. DSS was built by using the tool 'Borland Delphi' version 5. A total of 562 questions were formulated, including 322 rules, besides photographs to help in the diagnosis of 34 diseases, 40 pests and 34 abiotics disturbances in citrus. DSS evolution was divided into verification and validation. In the verification phase, DSS was analyzed by means of a questionnaire applied to citrus specialists. The program validation phase

  20. Mise en oeuvre de carottages de sol et de minirhizotrons pour l'étude à long terme de la réponse des fines racines d'épicéa (Picea abies (L. Karst. à l'augmentation de la concentration en CO2 dans l'atmosphère et la nutrition minérale

    Geneviève Pissart


    Full Text Available Use of soil cores and minirhizotrons for the long-term study of the response of Norway spruce fine roots (Picea abies (L. Karst. to the increase in CO2 concentration in the atmosphere and nutrition. The fine root biomass of Norway spruce (Picea abies (L. Karst. grown in open top chambers (OTCs under elevated CO2 since 1987 was investigated with soil cores and minirhizotrons, at Vielsalm (Ardenne, Belgium. Four levels of CO2 concentration (700, 580, 470 and 350 µmol.mol-1 CO2 were applied in combination with two nutrition regimes: the optimal nutrition and the nutrition corresponding to the local brown forest soil. First soil coring was performed in 1997 to describe the root biomass and root distribution according depth, at the initial stage of this study. Soil coring was repeated in 2003. Two observation and measurement campaigns using minirhizotrons were carried out in 2001 and in 2002. These latter measurements shown a very large proportion of very fine roots within the root classes classically reported in the literature: ]0-1 mm], ]1-2 mm], ]2-5 mm] and > 5 mm. Among the 2252 roots we regularly observed in minirhizotrons, more than 90 % were thinner than 0.5 mm in diameter, less than 2 % were in the range [1-3 mm[ in diameter and their mean was 0.28 mm. Calculations on the 42 Biotechnol. Agron. Soc. Environ. 2004 8 (1, 41-53 Lebègue C., Laita É., Perrin D., Pissart G. roots observed with minirhizotrons shown that root biomass under elevated atmospheric CO2 concentrations (580 and 700 µmol.mol-1 CO2 were two times higher than root biomass under ambient concentration (350 µmol.mol-1 CO2. As these figures are based on specific root length (SRL, they are supposed to be largely underestimated. A larger proportion of these roots were exploring the upper soil horizons under elevated CO2. Norway spruce showed an annual production cycle of fine roots, independent from the atmospheric CO2 concentration. Increased CO2 affected root branching. Under

  1. Elevation of Anopheles Chiriquiensis from Synonymy with Anopheles Parapunctipennis and Designation of Name-bearing Types for Anopheles Parapunctipennis and Anopheles Parapunctipennis Guatemalensis (Diptera: Culicidae)


    la determination de las especies y subespecies, basada en 10s caracteres de las hembras adultas. Ciencia (Mex. City) 6: 69-77. Russell, P.F., L.E...66-68. 186 Vargas, L. 1940b. Clave para identificar las Inst. Salubr. Enferm. Trop. 1: 199-203. hembras de Aszopheles mexicanos. Rev. Vargas, L...1941. Nota sobre 10s huevecillos de Atzopheles mexicanos. Gac. Med. Mex. 71: 107-123. Vargas, L. 1942. Las hembras Americanas de1 subgenero

  2. Parastās egles Picea abies L. ietekme uz mālsmilts augsnes virskārtas īpašībām bijušajās lauksaimniecībā izmantojamā zemē Taurenes apkārtnē

    Ševčuka, Aleksandra


    Latvijā, kā arī citās Eiropas valstīs pēc 1990. gada strauji palielinājās pamesto lauksaimniecībā izmantojamo zemju(LIZ) platības. Zemju neapsaimniekošanas dēļ uz bijušajām LIZ attīstījās sekundārā sukcesija un apmežošanas process. Apmežošanas izraisa veģetācijas, mikroklimata un augsnes īpašību izmaiņas uz bijušajām LIZ zemēm. Pētījuma mērķis ir noskaidrot egles (Picea abies L.) ietekmi uz augsnes īpašībām (ķīmiskajām, morfoloģiskajām, fizikālajām) Taurenes apkārtnē, Vidzemē. Bakalaura darbā...

  3. Responses of leaf functional traits and nitrogen and phosphorus stoichiometry in Abies fabiri seedlings in Gongga Mountain to simulated nitrogen deposition%峨眉冷杉幼苗叶片功能特征及其N、P化学计量比对模拟大气氮沉降的响应

    羊留冬; 王根绪; 杨阳; 杨燕


    Abies fabiri is one of the dominant conifers in the subalpine area of western Sichuan Province, Southwest China. Taking A. Fabiri seedlings as test materials, this paper studied their leaf functional traits and N and P stoichiometry under effects of simulated nitrogen deposition. To simulate nitrogen deposition, ammonium nitrate solution (50 kg N ? Hm-2? A-1) was weekly sprayed over the growth season (from May to October) of 2009 and 2010. After the two growth seasons, the total biomass, leaf dry weigh, leaf mass ratio, leaf N and P concentrations, and leaf N:P ratio of the seedlings under ammonium nitrate spraying increased by 11. 29% , 46. 70% , 41.40% , 37. 30% , 22. 33% and 6. 43% , respectively, while specific leaf area (SLA) de creased by 6. 61% , as compared with the control. There existed significant differences in the leaf dry weigh, leaf mass ratio, leaf N concentration, and leaf N : P ratio between ammonium nitrate spraying and the control, and the leaf dry weight had a strong linear correlation with leaf N con centration. The total biomass, leaf dry weight, leaf N and P concentrations, and leaf N : P ratio of seedlings under ammonium nitrate spraying were higher in the second growth season than in the first growth season. This study demonstrated that nitrogen deposition promoted the leaf growth of A. Fabiri seedlings significantly, and increased the leaf N and P concentrations and leaf N : P ratio. However, the variation of leaf N : P ratio under nitrogen supply suggested that the seedling growth was still limited by the shortage of N supply. It was suggested that A. Fabiri seedlings had cumulative effect for N.%通过人工施氮模拟大气氮沉降,研究了施氮对峨眉冷杉(Abies fabiri)幼苗叶片功能特征、氮和磷含量及其化学计量比的影响,以及幼苗对氮素的积累效应.结果表明:经过2个生长季节的施氮处理(2009年和2010年,N2)幼苗的总生物量、叶干重、叶重比、叶片氮和磷含量及

  4. Comparison of biotic and abiotic parameters between a native secondary forest fragment and a Pinus taeda L. reforestation Comparação de parâmetros bióticos e abióticos entre fragmento de floresta secundária nativa e um reflorestamento de Pinus taeda L.

    Talita Parpinelli Ferracin


    Full Text Available The substitution of natural forests for commercial forest plantations can be as damaging as farming or urban occupation. However, studies on natural regeneration in these areas show results in the development of native species in the understory, suggesting a successional process in favor of restoring biodiversity as well as an alternative forrestoration. This work aimed at comparing biotic and abiotic parameters between native secondary forest fragments and Pinus taeda reforestation. The study sites are located at Klabin Ecological Park, Monte Alegre Farm, Telêmaco Borba, Paraná. The following biotic data were evaluated: total height, diameter at breast height (DBH - 1.30 m above the ground and basal area (BA, in tree species with DBH ≥ 2.5 cm. The following abiotic data were also evaluated: pH, soil compaction, herb cover, canopy cover, litter thickness, air temperature, and relative humidity. Most parameters differed between pine reforestation and secondary forest, such as pH and soil compaction, canopy cover, herb presence and the size structure of vegetation (diameter and height. It was also observed a low regeneration in reforestation, evidenced by the difference in the basal area. However, there was regeneration in pine reforestation, suggesting that, over time, there will be an increase in the regeneration of the native vegetation, increasing the wealth of species and the structural complexity of the vegetation. A substituição das florestas naturais por plantios florestais comerciais, pode ser tão prejudicial quanto a agricultura ou a ocupação urbana. Porém, estudos sobre regeneração natural nessas áreas, apresentam resultados no desenvolvimento de espécies nativas no sub-bosque, sugerindo um processo sucessional favorável à recuperação da biodiversidade e também uma alternativa para a restauração. Este estudo teve como objetivo comparar parâmetros bióticos e abióticos entre fragmento de floresta secundária nativa

  5. Influência dos fatores abióticos e da disponibilidade de presas sobre comunidade de serpentes do Planalto Médio do Rio Grande do Sul Influence of abiotic factors and availability of prey on the occurrence of snakes in the middle plateau region of Rio Grande do Sul, Brazil

    Noeli Zanella


    Full Text Available A influência dos fatores abióticos sobre a disponibilidade de presas e a dieta das espécies de serpentes mais abundantes do Planalto Médio do Rio Grande do Sul, foi estudada em duas áreas: floresta e campo. O trabalho foi desenvolvido utilizando serpentes coletadas com os métodos: procura limitada por tempo, encontros ocasionais, armadilhas de interceptação e queda, e serpentes depositadas na coleção de répteis da Universidade de Passo Fundo. Foram registradas as guildas alimentares das seis espécies mais abundantes: anurófagas (n = 2: Echinanthera cyanopleura (Cope, 1885 e Thamnodynates cf. strigatus (Günther, 1858; rodentívoras (n = 1: Bothrops alternatus Duméril, Bibron & Duméril, 1854; moluscófagas (n = 1: Tomodon dorsatus Duméril, Bibron & Duméril, 1854 e generalistas (n = 2: Liophis poecilogyrus (Wied-Neuwied, 1825 e Philodryas patagoniensis (Girard, 1858. Dos fatores abióticos analisados, a abundância de serpentes foi mais relacionada à temperatura máxima (R² = 0,66 e não apresentou relação significativa com a pluviosidade. A abundância de anfíbios apresentou relação positiva com a pluviosidade (R² = 0,54 e não foi significativa com a temperatura mínima. A abundância de serpentes não foi correlacionada com a abundância de anfíbios e roedores.The influence of abiotic factors on the availability of prey and on the diet of the most abundant snake species in the middle plateau region of Rio Grande do Sul was studied in two areas: forest and field. Snakes captured using time-constrained search, occasional encounters and pitfall traps and snakes deposited in the reptile collection of the University of Passo Fundo were studied. The feeding guilds of the six most abundant species were recorded: anurophagous (n = 2: Echinanthera cyanopleura (Cope, 1885 and Thamnodynates cf. strigatus (Günther, 1858; rodent-eating (n = 1: Bothrops alternatus Duméril, Bibron & Duméril, 1854; molluscophagous (n = 1: Tomodon

  6. Walter Benjamin: un melanconico allievo di Aby Warburg

    Marco Bertozzi


    Full Text Available Walter Benjamin tried to get in touch with Panofsky and the Warburg’s circle, but the attempt failed. This article examines the chapter on melancholy of Benjamin’s The Origin of the German Tragic Drama (1928 and his main sources, i. e. Warburg’s essay Pagan-Antique Prophecy in Words and Images in the Age of Luther (1920 and Panofsky-Saxl’s work Dürers Melencolia I (1923. Benjamin interpreted the melancholy of the German Tragic Drama as a jump back to the deadly sin of sloth: he saw the saturnine melancholy under the sign of the medieval acedia.

  7. Naine ootab ettevõtluses abi / Signe Kalberg

    Kalberg, Signe, 1959-


    Mittetulundusühing ETNA e. Ettevõtlikud Naised Eestimaal juhatuse esimees Kaja Keskküla selgitas Järvamaa naisettevõtjaile FEM-kavandit, mille eesmärgiks on luua kolme aasta jooksul Balti mere riikides võrgustik ja jätkusuutlik infrastruktuur naisettevõtluse toetuseks. Diagramm: FIEde arv Eestis ja Järvamaal 01.11.2004. Tabel: Naiste osa (%) ettevõtjate hulgas 2004. a.

  8. Lanostane- and cycloartane-type triterpenoids from Abies balsamea oleoresin

    Serge Lavoie


    Full Text Available Phytochemical analysis of A. balsamea oleoresin led to the isolation of three new 3,4-seco-lanostane triterpenoids 1–3, one new cycloartane triterpenoid 4 along with fourteen known terpenoids. Structure determinations were based on extensive 1D/2D NMR, IR and MS spectroscopic analyses, and comparison with literature data. The isolated compounds were evaluated in vitro for their cytotoxicity against human cell lines (A549, DLD-1, WS1 and their antibacterial activity against E. coli and S. aureus. Abiesonic acid (6 exhibited weak cytotoxic activity against A549 (IC50 = 22 µM while compounds 1 and 4 were weakly active against S. aureus (MIC = 25 µM.

  9. Inimene peab abi saama kohe / Kalle Laanet ; interv. Jaanus Kõrv

    Laanet, Kalle, 1965-


    Siseminister maavanemate positsiooni nõrgenemisest, riigi äärealade olukorrast, maavanemate ja maavalitsuste vajalikkusest, siseministeeriumi haldusalas olevate ametite, andmekaitseinspektsiooni, Sisekaitseakadeemia ning Muraste piirivalvekooli tegevusest, päästeteenistuste olukorra parandamisest, siseministri tähtsamatest ülesannetest

  10. Management of silver fir (Abies alba Mill.) in Slovenia

    Bončina, Andrej; Ficko, Andrej; Klopčič, Matija; Matijašič, Dragan; Poljanec, Aleš


    In the paper, we analysed the structure and developmental characteristics of forest stands with silver fir in Slovenia, the management and cut in four forest site strata, where silver fir occurs. We used databases from the Slovenia Forest Service. In growing stock (GS) of silver fir, large (dbh=30-49cm) and very large (d=50 cm and more) diameter trees account for 84.9 % at the national level. The highest share of very large diameter silver fir trees (45 %) is in Dinaric silver fir forests and...

  11. Contribution of soil fauna to mass loss of Abies faxoniana leaf litter during the freeze-thaw season%季节性冻融期间土壤动物对岷江冷杉凋落叶质量损失的贡献

    夏磊; 吴福忠; 杨万勤


    冬季凋落物的质量损失是中高纬度和高海拔地区凋落物分解的关键,但冬季凋落物分解是否与土壤动物的贡献有关,不同冻融时期(冻融初期、深冻期和融化期)的土壤动物对凋落物分解的贡献是否存在差异?对这两个问题仍缺乏必要的关注.为了解季节性冻融期间土壤动物对岷江冷杉(Abies faxoniana)凋落物分解的贡献,采用凋落物分解袋法,调查了季节性冻融期间(2010年10月底至2011年4月中旬)’不同网孔(0.020 mm、0.125 mm、1.000mm和3.000 mm)凋落物分解袋内的岷江冷杉凋落叶质量损失,分析了微型、中型和大型土壤动物对岷汀冷杉凋落叶分解的贡献.在季节性冻融期间,0.020mm、0.125mm、1.000mm和3.000mm分解袋内的岷江冷杉凋落叶质量损失率分别为12.13%、13.07%、14.95%和18.74%.不同体径的土壤动物对季节性冻融期间岷江冷杉凋落叶质量损失的贡献率总共为35.28%;不同孔径凋落物袋内土壤动物的类 ,群和个体相对密度与凋落叶的质量损失率呈现相对·致的变化趋势.在季节性冻融的3个阶段中,十壤动物对岷江冷杉凋落叶质量损失的贡献率均为:微型土壤动物<中型土壤动物<大型土壤动物.其中,微型、中型和大型土壤动物分别在深冻期、冻融初期和融化期表现出最高的贡献率,分别为6.56%、11.77%和21.94%.然而相对于其他冻融时期,深冻期中型和大型土壤动物对岷汀冷杉凋落叶质量损失的贡献率最低.这些结果清晰地表明了川西高山季节性冻融期间土壤动物调控着凋落物分解的生态过程,是高山冬季凋落物分解的重要因素之一.%Aims Mass loss in wintertime is one of the key processes in litter decomposition in cold biomes. The contribution of soil fauna to litter decomposition has been unclear, and the contribution might be different in different winter periods (OF: the onset of the freeze

  12. Invertebrates associated to Eichhornea azurea Kunth in a lagoon of the Upper Paraná River: composition, community attributes and influence of abiotic factors Invertebrados associados à Eichhornea azurea Kunth em uma lagoa do alto rio Paraná: composição, atributos da comunidade e influência de fatores abióticos

    Valéria Flávia Batista-Silva


    ciclo hidrológico, bem como a possível influência de fatores abióticos sobre estes atributos. MÉTODOS: Amostragens foram realizadas em bancos de E. azurea durante os períodos chuvoso e seco em 2010. Os atributos avaliados foram abundância, riqueza, diversidade, equitabilidade e dominância. As variáveis limnológicas temperatura, oxigênio dissolvido, pH e turbidez foram sumarizadas através da Análise de Componentes Principais (PCA. Para verificar possíveis diferenças entre as médias dos atributos e dos escores do eixo 1 da PCA nos diferentes períodos utilizou-se análises de variância de modelos nulos. A influência dos fatores abióticos sobre cada atributo foi avaliada através de correlações de Pearson. RESULTADOS: Nós capturamos 3052 indivíduos, distribuídos em 32 táxons, pertencentes aos filos Mollusca, Annelida, Nematoda e Arthropoda. Dentre os atributos avaliados, apenas a abundância e riqueza variaram significativamente entre os períodos, com maiores valores obtidos para o chuvoso. Chironomidade foi dominante em ambos os períodos enquanto Notonectidae e Cyclopoida foram raros no período chuvoso e Bivalve, Decapoda, Haliplidae, Trichoptera e Pyralidae no período seco. Distinção temporal foi evidente apenas para o eixo 1 da PCA, o qual representou gradientes de temperatura, oxigênio dissolvido e pH. Dentre os atributos avaliados, somente a abundância apresentou correlação significativa e negativa com este eixo. CONCLUSÃO: Ficou evidente que: i o período chuvoso deve agregar condições favoráveis para a ocorrência de maior riqueza e elevada abundância de invertebrados nesta macrófita; ii somente este último atributo foi influenciado pelos gradientes limnológicos.

  13. Influência de diferentes sombreamentos e nutrição foliar no desenvolvimento de mudas de Palmeiras Ráfia Rhapis excelsa (Thunberg Henry ex. Rehder Influence of differents shading and fertilizer foliar in development of seedlings of lady palm Rhapis excelsa (Thunberg Henry ex. Rehder

    Antônio José Arantes Meirelles


    Full Text Available A palmeira ráfia (Rhapis excelsa, possui elevado valor comercial, sendo utilizada na composição de vasos e jardins, principalmente em interiores. No entanto, poucas são as informações sobre sua exigência em adubação ou mesmo sobre as condições ótimas de sombreamento. Para sombreamento, além do tradicional sombrite, estão disponíveis atualmente no mercado as malhas fotoconversoras, que estimulam o desenvolvimento das espécies vegetais. Objetivou-se nesse trabalho avaliar a influência de diferentes porcentagens e tipos de sombreamentos e a nutrição foliar no desenvolvimento de mudas desta palmeira. Utilizaram-se plantas com aproximadamente 4 anos, em vaso contendo terra, areia e esterco (1:1:1, acrescido de 10 g de superfosfato simples por litro de substrato. Determinou-se o incremento em altura e o número de folhas aos 105 e 195 dias. Foi determinado também o número e altura dos brotos, largura do folíolo e altura final das plantas aos 195 dias. Em relação à adubação foliar, não foi observado efeito dessa sobre o desenvolvimento das mudas. As malhas para sombreamento também não exerceram efeitos significativos na altura final de plantas, indicando que o desenvolvimento das mudas de palmeira ráfia pode ser realizado sob qualquer tipo de sombreamento.Lady palm (Rhapis excelsa has high commercial value, specially in ornaments of vases and gardens located on shade. However, the information about its requirements in fertilizer and great terms of shading are little. To shade, the common used material is shade net black. The color shade net stimulate the vegetal growth and it is recently used in the commercial system. The aim of this work was to evaluate the influence of different percents and types of shades and foliar fertilizer in development of seedlings this palm. The plants used in this experiment were cultivated in recipients with soil, organic matter and sand (1:1:1, with the addition of 10 g of fertilizer by liter of substrate. These plants were four years old approximately. The measurements of increasing of height and number of leaves were taken at 105 and 195 days since the beginning of the experiment. It was also observed the number and height of seedlings, width from leaflets, as well as the final plant height at 195 days. The foliar fertilizer was not affected in growth of seedlings. The different types of screening either affect significantly the final height of the plants, indicating than the growth of palm Rhapis can be under any kind of shade.

  14. Efeitos de nitrogênio, fósforo e potássio no crescimento de Rhapis excelsa (Thunberg Henry ex. Rehder (Palmeira-Ráfia Effects of nitrogen, phosphorus and potassium on early growth of seedlings of Rhapis Excelsa (Thunberg Henry ex. Rehder (Lady Palm

    Petterson Baptista da Luz


    Full Text Available Rhapis excelsa é uma das palmeiras ornamentais mais cultivadas no mundo, indicadas principalmente para vasos e áreas de sombra. A maioria das palmeiras apresenta crescimento lento. O objetivo deste estudo é acelerar o processo de produção de mudas. Analisaram-se os efeitos da adubação realizada com nitrogênio, fósforo e potássio, sob o crescimento da espécie, ao longo de 8 meses. As mudas com altura de 3,80 cm foram plantadas em substrato constituído de partes iguais de terra e vermiculita. Realizaram-se aplicações semanais de NPK, diretamente no solo, na concentração de 10 mg.L-1 de substrato, utilizando-se como fonte: MAP (P, sulfato de amônio (N e cloreto de potássio (K. Os tratamentos foram constituídos de todas as combinações possíveis destes nutrientes, mais uma testemunha. O experimento foi mantido em casa-de-vegetação com nebulização. Avaliaram-se os resultados com base na produção de matéria seca da parte aérea e das raízes, área foliar, diâmetro do caule, número de folhas e altura do estipe. O nutriente que proporcionou um maior desenvolvimento e crescimento da palmeira Rhapis excelsa foi o nitrogênio, influenciando de forma positiva na maioria dos parâmetros analisados. Verificou-se melhor desenvolvimento das mudas com a aplicação de N, independente da combinação com P e ou K.Rhapis excelsa is one of the most cultivated ornamental palms of the world, indicated for cultivation in pots and under shadow. Most of the palm trees present slow growth. The objective of this study was to investigate the influence of fertilization on acceleration of palm seedlings production. The effects of nitrogen, phosphorus and potassium fertilizers on the growth of seedlings of this specie, during 8 months, were investigated in this work. Seedlings with 3.80 cm of height were cultivated in a substrate constituted of equal parts of soil and vermiculite. Applications of NPK were made weekly, at the concentration of 10 mg.L-1 of substrate, using MAP, ammonium sulphate and potassium chloride. The treatments were composed of all combinations of these mineral nutrients plus one control. The experiment was carried out in a green house with sprinkles. Dry weight production of shoots and roots, leaf area, stalk diameter, number of leaves and plant height were measured. Nitrogen fertilization increased the development and growth of the seedlings, raising most of the analyzed parameters. Better seedling development was observed with application of N, but there was no correlation between N and P or K application.

  15. Desenvolvimento de Rhapis excelsa (Thunberg Henry Ex. Rehder (Palmeira-Ráfia: Influência da altura do recipiente na formação de mudas Development of Rhapis excelsa (Thunberg Henry Ex. Rehder (Palm-Ráfia: influence of the height of the recipient in the formation of seedlings

    Petterson Baptista da Luz


    Full Text Available Rhapis excelsa, originária do sul da China, é uma das palmeiras ornamentais mais cultivadas no mundo, principalmente em vaso. Objetivou-se com esta pesquisa investigar a influência da altura do vaso no desenvolvimento da espécie. Para tanto, mudas de dois anos de idade, apresentando 10 cm de altura e sistema radicular com 10 cm de comprimento, foram transplantadas para recipientes de PVC com 10 cm de diâmetro e 20, 25, 30, 35 e 40 cm de altura, que se constituíram em tratamentos. O substrato utilizado foi uma mistura de casca de pinho, vermiculita e solo argiloso (2:1:1, adubado com 45 g de Osmocote (15:10:10. Os parâmetros avaliados foram: altura da planta, diâmetro do colo, número de folhas, número de perfilhos emitidos, avaliados aos 6, 12 e 18 meses após a instalação do experimento. Aos 18 meses foram tomados também o peso da matéria seca da parte aérea e o peso da matéria seca das raízes. O delineamento experimental foi inteiramente casualizado, com cinco repetições com duas plantas. Houve diferença significativa para a variável altura de plantas, aos 6 meses, sendo que o recipiente de 40 cm de altura apresentou o pior resultado. Não houve diferença significativa entre as variáveis analisadas aos 12 meses de investigação. Já aos 18 meses, houve diferenças significativas entre os tratamentos para as variáveis altura da planta e diâmetro do colo, sendo o recipiente de 40 cm inferior aos demais em relação à altura da planta e o de 25 cm superior quanto ao diâmetro do colo. A utilização do recipiente com 25 cm de altura revelou-se interessante pela qualidade das plantas formadas e em relação a aspectos econômicos da produção.Rhapis excelsa is one the most important ornamental palm cultivated in the world, mainly as vase plant, being indigenous to China. The aim of this research was to investigate the influence of vase height on the development of the species. Two years-old seedlings with 10 cm height and 10 cm root system length were transplanted to PVC containers with 10 cm diameter and 20, 25, 30, 35 and 40 cm height. The vase substratum was a mixture of pine bark, vermiculite and soil (2:1:1, fertilized with 45 g of Osmocote (15:10:10. The evaluated parameters were: plant height, stem based diameter, number of leaves and numbers of shoots, at 06, 12 and 18 months after the beginning of the experiment. For 18 months plants, the foliar dry matter weight and the roots dry matter weight were also evaluated. The experimental design was completely randomized with five replicates and each experimental unit was composed of 2 plants. There was a significant difference for the parameter plants height at 6 months, and the 40 cm height container presented the worst results. There was no significant difference among the treatments at 12 months since transplantation. After 18 months, there were significant differences among the treatments for the parameters plant height and diameter of the lap, with plant height being lower for the 40 cm container than other treatments and the diameter of the lap being greater for 25 cm container. The use of 25 cm height containers was interesting because of the quality of the grown plants and in relation to the economical aspects of the production.



    Авторами статьи изучено влияние стимулятора (регулятора) роста «Крезацин» на выращивание в лесном питомнике двулетних сеянцев пихты почкочешуйной (белокорой) (Abies nephrolepis Maxim.) и пихты цельнолистной (Abies holophulla Maxim.). Установлено, что корневая подкормка водным раствором стимулятора концентрацией 1 мл/10 л активизирует рост сеянцев по длине мочки корня, диаметру корневой шейки и высоте. Положительное влияние на сеянцы более низких концентраций раствора не отмечено....

  17. Needle longevity, photosynthetic rate and nitrogen concentration of eight spruce taxa planted in northern Japan.

    Kayama, Masazumi; Kitaoka, Satoshi; Wang, Wenjie; Choi, Dongsu; Koike, Takayoshi


    Growth characteristics of Picea glehnii Masters, P. jezoensis (Sieb. et Zucc) Carr., P. jezoensis var. hondoensis (Mayr) Rehder and P. shirasawae Hayashi from Japan, P. abies (L.) Karst. from Europe and P. glauca Voss, P. mariana Britt., Sterns and Pogg. and P. rubens Sarg. from North America were compared. The trees were grown in similar conditions at the Tomakomai Experimental Forest of Hokkaido University in northern Japan. Tree growth, needle biomass, longevity, photosynthetic rate, nitrogen concentration and specific leaf area (SLA) were measured, and photosynthetic nitrogen-use efficiency was calculated. Picea jezoensis, P. jezoensis var. hondoensis, P. abies and P. glauca had high growth rates, high photosynthetic rates in young needles, high needle nitrogen concentrations and short needle life spans. In contrast, P. glehnii, P. shirasawae, P. mariana and P. rubens had low growth and photosynthetic rates, low needle nitrogen concentrations, long needle life spans and maintained a high photosynthetic nitrogen-use efficiency in older needles. Examination of relationships between several growth parameters of the eight taxa revealed positive correlations between SLA and mass-based photosynthetic rate and between SLA and mass-based nitrogen concentration, whereas mass-based photosynthetic rate and mass-based nitrogen concentration were negatively correlated with needle longevity. The species differed greatly in growth characteristics despite being grown in similar conditions.

  18. Relationships of Stable Carbon Isotope of Abies faxoniana Tree-Rings to Climate in the Sub-Alpine Forest in Western Sichuan%川西亚高山森林岷江冷杉树轮碳稳定同位素对气候要素的响应

    靳翔; 徐庆; 刘世荣; 姜春前


    利用四川卧龙亚高山暗针叶林岷江冷杉树木年轮样本资料,建立树轮宽度年表,对比宽度年表,提取树木年轮(简称树轮)碳稳定同位素(δ13C)序列和去趋势序列(DS),研究岷江冷杉树轮碳稳定同位素序列对气候要素的响应关系.结果表明:岷江冷杉(1904-2009年)树轮碳稳定同位素变化范围为-23.33‰~-26.31%‰,平均值为-24.91‰,变异系数为-0.025;相关分析表明,岷江冷杉δ13C序列(DS)与前一年11月和当年1月的月平均气温显著正相关(P≤0.05),与前一年1月和当年2,11月的月平均气温极显著正相关(P≤0.01),冬季平均气温对岷江冷杉树轮碳稳定同位素的响应最为敏感,是研究过去环境变化的良好载体,与当年1月降水量显著正相关(P≤0.05),与全年的月平均相对湿度相关性不显著(P≥0.05).%The tree-ring stable isotope technique is an important means to reconstruct climate chronology and to determine tree responses to environmental factors.In this study,the tree-ring samples of Abies faxoniana,collected in 2010 in a sub-alpine forest of Wolong Sichuan,were used to develop the tree-ring width chronology and the stable carbon isotope was extracted based on the standard dendrochronology methods.This new approach can be used to establish the detrend series (DS) of stable carbon isotope of tree rings to eliminate effect of the 13C originated from atmospheric CO2.Results showed that the stable carbon isotope series (1904-2009) varied in a range of-23.33‰--26.31%o with the average value of-24.91‰ and the coefficient of variation of-0.025.Correlation analysis revealed that the tree-ring DS values were significantly positively correlated to the mean monthly temperature of the last January,and the February and November of the current year (P≤0.01),and was also correlated to the mean monthly temperature of the last November and the January of the current year (P ≤ 0.05).The DS values were positively

  19. Responses of the Tree-Ring of Abies faxoniana and Tsuga chinensis to Climate Factors in Sub-Alpine in Western Sichuan%川西亚高山岷江冷杉和铁杉年轮对气候因子的响应

    靳翔; 徐庆; 刘世荣; 姜春前


    研究四川卧龙亚高山森林岷江冷杉和铁杉树木年轮与气候因子的关系.结果表明:川西亚高山针叶林树木径向生长主要受气温的制约,卧龙地区岷江冷杉树轮宽度序列与当年2和4月月平均气温显著正相关(P≤0.05),而与多数月平均降水量和月平均相对湿度负相关;铁杉树轮宽度序列与上一年7月和当年2--4月月平均气温显著正相关(P≤0.05),与上一年10月和当年5月月平均降雨量显著正相关(P≤0.05),而与上一年7月及当年4和9月月平均相对湿度显著负相关(P≤0.05).%By using the tree-ring samples of Abies faxoniana and Tsuga chinensis collected from sub-alpine forests in Wolong Sichuan in 2010, the tree-ring width standard chronologies and residual chronology were established, A. faxoniana and T. chinensis tree-ring chronology in this region contains worthy climate information. The calculation results showed that A. faxoniana and T. chinensis tree-growth and tree-ring width chronologies were significantly positively correlated with the mean air temperature, suggesting that it was the restriction factor for the tree growth. In study area, the tree-ring width of A. faxoniana was positively correlated to the mean monthly temperature in current February and April (P≤0.05). The correlations of the tree-ring width of A. faxoniana with monthly precipitation and relative humidity were being negative with the lots of months. The tree-ring width of T. chinensis was positively correlated to the mean monthly temperature last July and current February to April (P≤0.05) and to the monthly precipitation in last October and current May (P≤0.05) , and was significantly negatively correlated with monthly relative humidity in last July and current April and September ( P≤0. 05 ) . A. faxoniana tree-ring width chronologies was not significantly correlated with season average climate factors; but T. chinensis tree-ring width was significantly positively

  20. Comparison between abiotic parameters and the forest structure of a forest fragment and an eucalyptus (Eucalyptus saligna Smith abandoned reforestation in the ecological park of Klabin, Telemaco Borba/PR Comparação entre parâmetros abióticos e a estrutura florestal de um fragmento de floresta e um reflorestamento abandonado de eucalipto (Eucalyptus saligna Smith no parque ecológico da Klabin, Telêmaco Borba/PR

    José Antonio Pimenta


    ções florestais, sendo uma delas um fragmento florestal secundário e a outra uma área abandonada de reflorestamento com eucalipto, neste trabalho foram avaliados alguns parâmetros abióticos, bem como a estrutura florestal das áreas. Dentre os fatores abióticos estudados estão: temperatura e umidade relativa do ar, compactação, umidade e pH do solo e espessura da serapilheira. Para analisar a estrutura florestal, foram avaliados: cobertura de herbáceas sobre o solo, índice de cobertura do dossel, diâmetro a altura do peito (DAP, área basal (AB e altura das árvores de todas as espécies. Entre os parâmetros analisados, comparando-se as áreas, foram observadas que: a compactação do solo é maior na floresta secundária (0,57 MPa do que no reflorestamento de eucalipto (0,43 MPa; a presença de herbáceas sobre o solo é menor no reflorestamento de eucalipto (17,96% do que na floresta secundária (59,4%; o índice de cobertura do dossel foi de 45,99% para o reflorestamento de eucalipto, enquanto na floresta secundária este valor foi de 61,02%. No entanto, de acordo com o teste de Kolmogorov-Smirnov, a estrutura florestal não é diferente entre as áreas, já que os valores de DAP e altura não diferiram entre o calculado e o observado.

  1. Variação do número de estômatos e micropêlos em Paspalum vaginatum Sw: em relação às condições abióticas numa marisma do estuário da Lagoa dos Patos, RS-Brasil Variation in the number of stomata and microhairs of Paspalum vaginatum Sw: en relation to abiotic conditions in a breakwater in the Lagoa dos Patos estuary, RS-Brazil

    Eleci de Oliveria Bastos


    Full Text Available Paspalum vaginatum Sw., gramínea perene, estolonífera, com folhas anfistomáticas e pequenas é característica de pântanos salgados, crescendo em condições estressantes na marisma da lagoa dos Patos, molhe oeste da Barra do Rio Grande, RS. Foram feitas contagens mensais de estômatos e micropêlos nas superfícies adaxial e abaxial das lâminas e relacionadas às características abióticas do ambiente. O número de micropêlos da superfície foliar abaxial variou significativamente e diretamente com a temperatura da água intersticial junto às rizosferas. Estes, porém, não mantiveram correlação com a salinidade intersticial. Os resultados sugerem que a espécie seja uma halófita facultativa. O número de estômatos da superfície abaxial manteve um relacionamento inverso com a pluviosidade. Paspalum vaginatum, apresenta-se como uma espécie com características xeromórficas.Paspalum vaginatum Sw., a perennial, stoloniferous grass with small leaves presenting stomata on both epidermises is characteristic of salt marshes, growing under stressful conditions near the west breakwater of the Rio Grande outlet, Lagoa dos Patos, Rio Grande do Sul, Brazil. Monthly counts of stomata and microhairs on the adaxial and abaxial surfaces were related to the abiotic characteristics of the enviroment. The number of microhairs of the abaxial leaf surface varied significantly and directly with interstitial water temperature close to the rhyzosphere. However, these counts did not correlate with the interstitial salinity. The results suggest that the species is a facultative halophyte. Stomata counts of the abaxial surface showed an inverse relation to precipitation. Paspalum vaginatum appears to be a species with xeromorphic characteristics.

  2. Climate-growth relationships of Abies nephrolepis and its connection with large-scale climate change in Xiaoxing'an Mountains, northeastern China%小兴安岭不同海拔臭冷杉年轮-气候关系及大尺度气候影响

    姚启超; 王晓春


    Three tree-ring standard chronologies of Abies nephrolepis at different elevations in Fenglin National Nature Reserve from Xiaoxing'an Mountains of northeastern China were developed by using standard dendrochronological methods. Relationships between these three chronologies and local ( mainly temperature and precipitation) and large-scale climatic factors were analyzed. Results showed that radial growth of A. nephrolepis in this region was mainly limited by air temperature, while the effect of precipitation was relatively weak. Three A. nephrolepis chronologies were negatively correlated with minimum and mean temperatures in the current growth season (P < 0. 01 ) and total precipitations in March (P < 0.05 ). Radial growth of A. nephrolepis in different elevations, however, responded differently to monthly mean temperatures and total precipitations. The effects of climatic factors ( monthly temperature and precipitation) on tree growth in high elevations were significantly stronger than that in low elevations. Correlation analysis between three A. nephrolepis chronologies and the index of Pacific Decadal Oscillation (PDO) and Atlantic Multidecadal Oscillation (AMO) indicated that radial growth of A. nephrolepis could be connected with large-scale climate changes in Pacific and Atlantic. A greater effect of AMO on tree growth appeared in low elevation sites, while greater effect of PDO on tree growth was in two high elevation sites. The decline of valley spruce-fir forests in this region in recent years could be related to the phase changes of AMO and PDO, which changed the regime of temperature and precipitation in this region. Higher minimum temperatures in growing season caused by large-scale climate ( AMO and PDO ) changes decreased net photosynthesis and increased respirations of A. nephrolepis in this area. Thus, the physiological recession of tree growth will appear. In addition, higher temperature may result in the disappearance of patchy permafrosts in

  3. Avaliação da atividade de indutores de resistência abiótica, fungicida químico e extratos vegetais no controle da podridão-negra em Abacaxi 'Pérola' Activity evaluation of abiotic resistance inducers, chemical fungicide and natural plant extracts on black rot of pineapple, cv. pérola

    Mônica Danielly de Mello Oliveira


    Full Text Available A podridão-mole ou podridão-negra, causada pelo fungo Chalara paradoxa (De Seyn. Sacc., é uma doença de pós-colheita que pode ser responsável por perdas elevadas, tanto em frutos para consumo in natura, quanto naqueles destinados à indústria de processamento. O corte do pedúnculo e ferimentos na casca do fruto favorecem a infecção. O objetivo do presente trabalho foi avaliar o efeito de indutores de resistência abiótica, fungicida e extratos vegetais no controle da podridão-negra em abacaxi. Os isolados foram obtidos de frutos coletados no município de Santa Rita, Paraíba, que apresentaram sintomas da doença. Utilizaram-se 32 frutos de cv. 'Pérola', lavados em água corrente e desinfestados com hipoclorito de sódio (produto comercial a 4,0%, por cinco minutos. Após secagem em temperatura ambiente, os frutos foram pulverizados com os tratamentos: 1 ADE (água destilada esterilizada; 2 Derosal; 3 BION® (Acibenzolar-S-Methyl; 4 Ecolife® (Quinabra; 5 Agro-Mos® (mananoligossacarídeo fosforilado; 6 extrato de alho a 20%; 7 extrato de cebola a 20%, e 8 extrato de nim a 20%. Os frutos tratados permaneceram em câmara úmida por 24 horas, antes da inoculação com um disco de micélio (6mm do fungo, incubado em BDA a 25±2ºC e fotoperíodo de 12 horas e colocado sobre um ferimento na região da casca. A avaliação do progresso da doença foi realizada seguindo-se escala de notas, onde: 1 - Ausência de sintomas; 2 - Podridãonegra em área da casca equivalente a 1-5 frutilhos; 3 - Podridão-negra em área da casca equivalente a 6-10 frutilhos; 4 - Podridão inicial da polpa com coloração pardo-amarelada; 5 - Podridão e desintegração da polpa atingindo área superior a 50% do fruto. O delineamento experimental foi o inteiramente casualizado, com oito tratamentos e quatro repetições, utilizando os modelos lineares generalizados com distribuição multinomial, sendo as médias comparadas pelo teste de Scott-Knott, a 5% de

  4. Efeito de fatores abióticos na larvicultura de pintado amarelo Pimelodus maculatus (Lacépède, 1803: salinidade e cor de tanque - DOI: 10.4025/actascianimsci.v26i2.1859 The effect of abiotic factors on the larviculture of pintado amarelo Pimelodus maculatus (Lacépède 1803: salinity an tank color - DOI: 10.4025/actascianimsci.v26i2.1859

    Marcos Weingartner


    Full Text Available Fatores abióticos podem determinar o sucesso na larvicultura de algumas espécies de peixes. A cor do tanque e salinidade da água de criação podem promover alterações no comportamento e na fisiologia das pós-larvas de peixes. O objetivo deste experimento foi avaliar a influência das salinidades 0,0‰; 0,7‰; 1,4‰; 2,0‰; 2,5‰ e 5,0‰ da água e das cores branca e preta dos tanques na larvicultura de Pimelodus maculatus. A sobrevivência foi afetada pela salinidade (p0,05 entre os tanques branco e preto com valor médio de 31,7±17,7%. Portanto, o valor de 2mg/L de NaCl re velou-se a melhor salinidade para larvicultura de Pimelodus maculatus, e as cores testadas não afetaram a sobrevivência das pós-larvas.Abiotic factors determine the success of the larviculture in some fish species. Tank color and the water salinity can cause alterations in the behavior and physiology of the fish post-larvae. The purpose of this experiment was to evaluate the influence of the difference water salinity concentration (0.0‰; 0.7‰; 1.4‰; 2.0‰; 2.5‰ and 5.0‰ and the use of white or black tanks in the larviculture of Pimelodus maculatus. The survival rate was affected by water salinity (p0.05 between post-larvae stocked in black or white tanks with an average value of 31.7±17.7%. Therefore, 2.0‰ proved to be the best salinity for larviculture of the Pimelodus maculatus, and the colors tested did not affect the survival of the post-larvae.

  5. Long-term research in Bosque Fray Jorge National Park: Twenty years studying the role of biotic and abiotic factors in a Chilean semiarid scrubland Investigación de largo plazo en el Parque Nacional Bosque Fray Jorge: Veinte años estudiando el rol de los factores bióticos y abióticos en un matorral chileno semiárido



    in this region, and that more frequent El Niños in conjunction with global climatic change may lead to marked changes in community dynamics. The importance of long-term experimental studies is underscored by the fact that only now after 20 years of work are some patterns becoming evident.Desde 1989 hemos llevado a cabo un experimento ecológico a gran escala en un matorral espinoso semiárido de un parque nacional en el norte de Chile. Inicialmente, nos centramos en el rol de las interacciones bióticas incluyendo depredación, competencia interespecífica y herbivoría en micromamíferos y componentes vegetales de la comunidad. Usamos una aproximación reduccionista con parcelas replicadas cercadas de 0.56 ha que selectivamente excluían depredadores vertebrados y/o micromamíferos herbívoros más grandes como el degu, Octodon degus. Aunque detectamos efectos transitorios menores en la sobrevivencia y número de degus en las exclusiones de depredadores, otras especies no mostraron respuestas. Similarmente, la competencia interespecífica (i.e., degus con otros micromamíferos no tenía efectos numéricos detectables (aunque ocurrieron algunas respuestas conductuales, y las exclusiones tuvieron efectos relativamente pequeños en varios componentes vegetales. Aproximaciones basadas en modelos indican que los factores abióticos juegan un papel determinante en la dinámica de las especies de micromamíferos principales como O. degus y la laucha orejuda (Phyllotis darwini. En cambio, estos están principalmente relacionados a pulsos no periódicos de lluvias más altas (usualmente durante los eventos El Niño que gatilla el crecimiento de plantas efímeras; un experimento de adición de alimento en 1997-2000 verificó la importancia de la precipitación como un determinante de la disponibilidad de alimento. Desde el 2004 hemos expandido los esfuerzos de monitoreo de largo plazo a otros componentes comunitarios importantes incluyendo aves e insectos con el fin de


    Kültiğin Çavuşoğlu


    Full Text Available Bu çalışmada, Kınkkale il ve ilçelerinde yaydış gösteren Pinus nigra subsp. nigra var. caramanica türününyapraklarında taşıtlardan yayılan kurşun birikiminin miktarı araştırılmıştır. Örnek toplanıa işlemi Kırıkkale il merkezive ilçelerinde yol kenarlarında belirlenen dokuz istasyondan gerçekleştirilmiştir. Her bir istasyondan toplanan yaprakörneklerindeki kurşun miktarlan elektron dağılım spektroskopi (EDS yardımı ile belirlenmiştir. En fazla kurşunbirikimine Kırıkkale il merkezinden (o/o44.169 toplanan yaprak örneklerinde rastlanılın ı ştır. Farklı ilçelerden toplananyapraklardaki kurşun miktarları karşılaştınldığında ise en fazla kurşun birikimi Bahşılı ilçesinden (%38.917, en azbirikim ise Karakeçili ilçesinden (%22.883 toplanan yaprak örneklerinde ölçülmüştür. Bu çalışma sonuçlarıyapraklardaki kurşun birikiminin trafık yoğunluğu ile arttığını ve P. nigra türünün bu birikimin tespitinde biyolojik birbelirleyici olarak kullanılabileceğini göstermiştir.

  7. Effects of Forest Gaps on Litter Lignin and Cellulose Dynamics Vary Seasonally in an Alpine Forest

    Han Li


    Full Text Available To understand how forest gaps and the associated canopy control litter lignin and cellulose dynamics by redistributing the winter snow coverage and hydrothermal conditions in the growing season, a field litterbag trial was conducted in the alpine Minjiang fir (Abies faxoniana Rehder and E.H. Wilson forest in a transitional area located in the upper reaches of the Yangtze River and the eastern Tibetan Plateau. Over the first year of litter decomposition, the litter exhibited absolute cellulose loss and absolute lignin accumulation except for the red birch litter. The changes in litter cellulose and lignin were significantly affected by the interactions among gap position, period and species. Litter cellulose exhibited a greater loss in the winter with the highest daily loss rate observed during the snow cover period. Both cellulose and lignin exhibited greater changes under the deep snow cover at the gap center in the winter, but the opposite pattern occurred under the closed canopy in the growing season. The results suggest that decreased snowpack seasonality due to winter warming may limit litter cellulose and lignin degradation in alpine forest ecosystems, which could further inhibit litter decomposition. As a result, the ongoing winter warming and gap vanishing would slow soil carbon sequestration from foliar litter in cold biomes.

  8. Annual and Monthly Variations in Litter Macronutrients of Three Subalpine Forests in Western China

    YANG Wan-Qin; WANG Kai-Yun; S.KELLOM(A)KI; ZHANG Jian


    Macronutrients (N, P, K, Ca, Mg, and S) in litter of three primarily spruce (Picea purpurea Masters) (SF), fir (Abies faxoniana Rehder & E. H. Wilson) (FF), and birch (Betula platyphylla Sukaczev) (BF) subalpine forests in western China were measured to understand the monthly variations in litter nutrient concentrations and annual and monthly nutrient returns via litterfall. Nutrient concentration in litter showed the rank order of Ca > N > Mg > K > S > P. Monthly variations in nutrient concentrations were greater in leaf litter (LL) than other litter components. The highest and lowest concentrations of N, P, K, and S in LL were found in the growing season and the nongrowing season, respectively, but Ca and Mg were the opposite. Nutrient returns via litterfall showed a marked monthly pattern with a major peak in October and one or two small peaks in February and/or May, varying with the element and stand type, but no marked monthly variations in nutrient returns via woody litter, reproductive litter, except in May for the BF, and moss litter. Not only litter production but also nutrient concentration controlled the annual nutrient return and the monthly nutrient return pattern. The monthly patterns of the nutrient concentration and return were of ecological importance for nutrient cycling and plant growth in the subalpine forest ecosystems.

  9. Chemotaxonomic significance of flavonoids, coumarins and triterpenes of Augusta longifolia (Spreng. Rehder, Rubiaceae-Ixoroideae, with new insights about its systematic position within the family Significância quimiotaxômica de flavonoides, cumarinas e triterpenos de Augusta longifolia (Spreng. Rehder, Rubiaceae-Ixoroideae, com novos entendimentos sobre a posição sistemática dentro da família

    Rafael Choze


    Full Text Available Augusta has traditionally been placed in the tribe Rondeletieae, subfamily Cinchonoideae. However, recent molecular phylogenies positioned it near to Wendlandia (Ixoroideae, but locate A. longifolia near to the clade Ixoroidinae II. The study of A. longifolia afforded two coumarins, five flavonoids, three triterpenoids and one benzoic acid derivative. These metabolites reinforce the separation of Augusta as a monospecific genus, and Lindenia as a genus of three species, closely related to Wendlandia.Augusta tem sido tradicionalmente colocada na tribo Rondeletieae, Cinchonoideae subfamília. No entanto, recentes filogenias moleculares posicionou-a perto de Wendlandia, porém localizando A. longifolia perto do clado Ixoroidinae II. O estudo de A. longifolia resultou em duas cumarinas, cinco flavonoides, três triterpenoides e um derivado do ácido benzóico. Estes metabolitos reforçam a separação da Augusta como um gênero monoespecífico, e Lindenia como um gênero de três espécies, intimamente relacionada com Wendlandia.

  10. Aluminium toxicity in seedlings of spruce (Picea abies (L. ) Karst) grown in culture broth. Aluminiumtoxizitaet bei Saemlingen der Fichte (Picea abies (L. ) Karst) in Naehrloesungskultur

    Jorns, A.C.


    The work aimed to study the changes that take place in spruce seedlings in water culture under conditions of aluminium toxicity (170-5200 Al; pH 3.8) and to verify whether these changes are comparable to the ones observed in damaged spruces in the field. Furthermore, it aimed to establish how the supply of nutrients influences the plant's aluminium tolerance and whether by supplying nutrients correspondingly such adverse effects through aluminium can be avoided. The following results were obtained: The untreated long and short roots of spruce belong to the root type of 'periblemo-calyptrogens'. The spruce seedlings proved exceptionally tolerant to aluminium; yet damage to roots and inhibition of root growth were observed even at relatively low aluminium concentrations (170-520 if nutrient supply was reduced. After 40 days of treatment with aluminium, needles began to show chloroses and red-brown discolouration; this was identified by means of needle analyses as lack of magnesium and slight lack of calcium. By increasing the total concentration and, especially, the magnesium concentration of the culture broth, the seedlings' tolerance to aluminium was increased. On treatment of the plants with 520 aluminium in dilute culture broth the roots showed the metacutinisation specific for aluminium toxicity. Supposedly, the zone of the root tip, an important one for apoplastic ion uptake, is reduced by the process of metacutinisation, resulting in inhibited magnesium and calcium transport.

  11. Toxic components of motor vehicle emissions for the spruce Picea abies.

    Kammerbauer, H; Selinger, H; Römmelt, R; Ziegler-Jöns, A; Knoppik, D; Hock, B


    Six-year-old Norway spruce trees were exposed for 30 min under standardised conditions to the exhaust from an Otto engine running on lead-free petrol. Gas-exchange measurements in an open system using an infrared gas analyser showed a sudden, severe drop in CO(2) assimilation and transpiration rates. By using filters which absorbed different fractions of the exhaust it could be demonstrated that the toxic effects can be attributed to the NO(x) fraction.

  12. Kriisimissioonidel ei anta üksnes abi, seal õpitakse palju ka ise / Ene Pajula

    Pajula, Ene, 1950-


    Lääne-Tallinna keskhaigla kirurgia- ja anestesioloogiakliiniku juhataja, anestesioloog Jaak Talving on üks Eesti kümmekonnast päästemeedikuks koolitatud arstist. Ta on käinud missioonil Indoneesias ja Haitil ning aidanud sisustada Afganistani väikehaiglat

  13. Stem and Crown Characteristics of Norway Spruce [Picea abies (L. Karst] Populations from Romanian Carpathians

    Marius BUDEANU


    Full Text Available To establish the most important Norway spruce Romanian populations, open-pollinated progeny of ten plus trees originating from 33 seed stands mapped in the Romanian Carpathians have been evaluated for stem and crown characteristics at the age of 30 years, in two field trials: one established in their natural range (Breţcu trial, while the second was located outside of their natural range, in Subcarpathian lands (Avrig trial. Significant (p < 0.05 differences were found among the seed stands in both sites for most of the characteristics, suggesting an additive genetic control with different levels of significance depending on the evaluated trait and the testing site. Location analysis also revealed a highly significant population x locality interaction for all traits. The same populations behave differently to the change of the ecological conditions. In the Avrig trial higher values were recorded than in the Breţcu trial for the tree slenderness coefficient (19%, crown slenderness coefficient (13.6% and crown lateral area (9.4%. Although outside of their natural area, pruning height (m increased 6.4%, however pruning height ratio (% decreases by 1%, compared to the test established in their natural range. The populations from the Eastern Carpathians stand out by showing sustained growth and superior wood quality, while the populations from the Western Romanian Carpathians have generally smaller differences between the mean values of analysed traits in both field trials. Qualitative characteristics that influence the stability of stands to windthrow and snowbreak showed low levels of correlations to ecological gradients of the originating location of tested seed stands.

  14. Soome sõlmis Kreekaga abi andmisel erikokkuleppe. Deposiit teenib intresse / Kadrin Karner

    Karner, Kadrin


    Soome ja Kreeka kokkuleppe kohaselt maksab Kreeka Soome riigi arvele tagatisraha, milla saab koos intressidega tagasi 2036 aastal, kui Kreeka on abipaketis osalenud riikidele kõik kohustused täitnud. Andres Arraku seisukoht

  15. Afganistan väärib meie abi / Stanley Davis Phillips

    Phillips, Stanley Davis


    Lääne toetus aitab Afganistani elanikel luua stabiilset, demokraatlikku ja majanduslikult elujõulist tulevikku. Kuigi töö Afganistanis on väga riskantne, on Eesti abiks sealsetel elanikel turvalise keskkonna loomisel

  16. Afganistan vajab liitlasvägede abi veel aastaid / Holger Roonemaa

    Roonemaa, Holger


    Kuigi liitlasväed tegutsevad Afganistanis juba märksa laiemal alal kui aastaid tagasi, on koalitsioonivägede kohalolek veel aastaid riigile vajalik. Taliban jätkab aktiivset vastupanu ning on alustanud ka infosõda. Kaart. Lisa: Võitlus südamete ja mõistuse pärast; Oopiumitoodang suureneb. Vt. samas intervjuud logistikaüksuse julgestusmeeskonna ülema vanemveebel Urmas Pindisega

  17. Haiti vajab suurt abi, et jalule tõusta / Heiki Suurkask

    Suurkask, Heiki, 1972-


    ÜRO palub maailmalt maavärinas kannatanud Haiti jaoks rohkem kui pool miljardit dollarit. EBRD ja Maailmapank on sel aastakümnel Haitile ülesehituseks andnud sadu miljoneid dollareid, USA on 5 aasta jooksul Haitisse investeerinud 800 mln. dollarit. Riiki on püütud reformida, kuid korruptsioonist pole vabanetud

  18. Una familia de ulemas cordobeses. los banu abi 'Isà


    El estudio de las grandes familiasandalusies puede contribuir a un mejor conocimiento de la sociedad hispano-árabe desde numerosos puntos de vista. El magnífico trabajo de don Elías Terés ((Linajes árabes en al-Andalus según la hmhara de Ibn ijazm»l ilustra perfectamente esta afirmación. Tanta atención como las familias nobles, sin embargo, merecen las de sabios, alfaquies o jurisconsultos, cuyo estudio es necesario para poder comprender mejor problemas tales como la tran...

  19. Growth rate of Heterobasidion annosum in Picea abies established on forest land and arable land

    Bendz-Hellgren, M.; Johansson, Martin; Swedjemark, G.; Stenlid, J. [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Mycology and Pathology; Brandtberg, P.O. [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Ecology and Environmental Research


    The growth rates of Heterobasidion annosum in Norway spruce were investigated in southern Sweden. In one study, stump and tree roots in stands established on previous forest or arable land were inoculated with H. annosum-infested sawdust. After 1-3 yrs, the linear extent of colonization by the fungus was measured, based on detection of its conidiophores on incubated samples. The average growth rate was 25 cm yr{sup -1} in stump roots and 9 cm yr{sup -1} in tree roots, neither of which differed significantly between forest and arable land. The feeling of a decayed tree could enhance the spread of H. annosum within root systems. In the second study, the height of discoloration and extent of colonization by H. annosum, measured as above, were assessed in naturally infected trees. On average, discoloration moved through the roots and stem at a rate of 36 cm yr{sup -1}. Heterobasidion annosum was found 60 cm in advance of the discoloration, corresponding to a growth rate of 52 cm yr{sup -1}.

  20. III-IV klassi abiõppeõpilaste vahendatud tekstiloome / Karl Karlep, Ana Kontor

    Karlep, Karl


    Artiklis kirjeldatakse abikooli III-IV klassi õpilaste tekstiloome arengut kahe õppeaasta jooksul (1998.-2000.a.). Taseme uurimiseks kasutati lühitekste, mida õpilased kuulasid ja suuliselt ümber jutustasid, analüüsiti jutustuste sisu ja keelelist vormistust. Eriti pakkusid huvi kasutatavad süntaktilised struktuurid, kuna esmakordselt võeti kasutusele harjutuste süsteem muuteoperatsioonide (lausete ühendamine, sisestamine jm.) õpetamiseks-harjutamiseks

  1. DEPREDACIÓN DE LAS SEMILLAS DE Abies religiosa (Kunth) Schltdl. et Cham


    La depredación en los bosques de coníferas es trascendente como proceso sustancial de su conservación, ya que su intensidad y las partes utilizadas definen cambios estructurales en los ecosistemas. El consumo de semillas, en particular, implica una fuerza de selección en virtud de que supone pérdida de germoplasma, por un lado, y, por otro, una nueva distribución del mismo. Con el propósito de conocer el efecto del forrajeo de semillas de oyamel sobre el potencial reproductivo de la especie, ...

  2. Lääne abi vajava Moldova toetamine on Eesti huvides / Marianne Mikko

    Mikko, Marianne, 1961-


    Euroopa Parlamendi Moldova delegatsiooni juht annab ülevaate olukorrast riigis. Autori sõnul nähakse Moldovas Transnistria probleemi lahendamise ainsat võimalust Lääne toetuses ning surves Ukrainale

  3. The identity of some taxa of Schizophragma (Saxifragaceae)

    Wei LIU; Xiang-Yun ZHU


    After examining a large number of type and authoritative specimens of Schizophragma Siebold & Zucc., S. elliptifolium C. F. Wei and S. integrifolium var. glaucescens Rehder were reduced to synonyms of S. hypoglaucum Rehder and S. integrifolium f. cordatum S. Y. Wang was reduced to synonym of S. corylifolium Chun. Schizophragma crassum var. ellipticum J. Anthony was treated as the correct name of S. crassum var. hsitaoanum (Chun) C. F. Wei. The name S. integrifolium var. minus Rehder is reinstated. The taxonomic treat-ment is supported by leaf epidermal characters.

  4. Sucesión y restauración ecológica en las partes altas de cuencas y la provisión de agua

    José Vicente Martínez Arévalo


    Full Text Available Se presenta la descripción del proceso de sucesión ecológica alrededor de parches de bosque de pinabete (Abies guatemalensis en la parte alta de San Marcos, Guatemala, y como a partir de esta se puede establecer un plan de restauración ecológica que permita mejorar los servicios ambientales de estos ecosistemas en especial en la provisión de agua de tal manera que contribuya a equilibrar el ciclo hidrológico permitiendo una escurrimiento más lento y mayor infiltración. El estudio muestra una riqueza de 85 especies clasificadas en estrato herbáceo inferior, estrato herbáceo superior, arbustos y árboles, en donde se muestra una dinámica de la vegetación a través de los cinco estadios sucesionales estudiados, sin haber una dominancia total de unas pocas especies. Se resaltar el papel ecológico de los musgos en la sucesión ecológica y en la retención de agua y humedad, en el estudio hay tres especies que se mantienencon altos valores de importancia a través de los cinco estadios sucesionales. Se identificaron arbustos como plantas nodrizas que juegan un papel importante en el cambio de la fisonomía de la vegetación ya que permiten el establecimiento de especies de árboles que llevan hacia lamadurez del ecosistema y contribuyen a la retención de la humedad en el suelo. Se enfatiza en la contribución que la vegetación tiene en la retención del agua y su mejoramiento en las partes altas de la cuenca, en donde el pinabete es uno de los ecosistemas de mayor importancia.

  5. Analysis of sesquiterpene distributions in leaves, branches, and trunks of avocado (Persea americana Mill.)

    Avocado is a commercially valuable fruit crop cultivated in tropical and subtropical climates throughout the world. Taxonomists recognize three horticultural races of avocado, consisting of Mexican (Persea americana var. drymifolia), Guatemalan (P. americana var. guatemalensis), and West Indian (P. ...

  6. Accumulation of radioactivity in mushrooms and its relation with the mycophagous rodents biology in an Abies religiosa forest; Acumulacion de radiactividad en hongos y su relacion con la biologia de roedores micofagos en un bosque de Abies religiosa

    Valenzuela G, V.H


    Nowadays the society expresses its concern by the action of the nuclear energy with respect to installations safety, the transport of radioactive materials, the radioactive waste management, nuclear accidents and nuclear tests. The concern is based to the fear of radioactive explosion which contaminates the environment and the damages to the public health. The mathematical models which establish and define the behavior and the exposure conditions of radioactive substances in the human beings as well as the systematic deposit in the terrestrial surface of the particles suspended in the air which contain radioactive material are called fallout, that is a contamination indicator. The objective of this work is to identify the little rodents species with greater accumulation of radionuclides of artificial origin. Due to the mushrooms are good bio indicators of radioactive contamination and the rodents consume them often, both are of greater assistance as indicators of this type of contamination and contribute to the knowledge of the {sup 137} Cs and {sup 40} K dynamics at the forest system. This work forms part of the environmental radiological surveillance of the Mexican Nuclear Center (CNM) in which are analysed several samples which will be used as biological indicators of radioactive contamination which serve for the transfer coefficient calculations of the different routes by which the radiation can to arrive to the human being, being able to be evaluated the dose to the people who live at the CNM surrounding area. Due to not always it is possible to detect so lowest levels of radioactivity in some environmental samples (air, water and, foods) it is necessary to quantify them in other animals or plants which accumulate them. (Author)

  7. Branching system and needle loss of spruce (Picea abies (L. ) Karst. ) as a basis of assessing forest decline. Das Verzweigungssystem und der Nadelfall der Fichte (Picea abies (L. ) Karst. ) als Grundlage zur Beurteilung von Waldschaeden

    Gruber, F.


    The book concentrates on the spruce's crown architecture, the regular and proventitious branching and the branching strategies. The morphology is looked at specially from an ecological point of view. Various types of needle loss in the spruces' crowns are described in detail covering anatomical studies as well as quantitative. Less conspicuous growth dimensions are quantified and thus complete the overall picture of spruce growth and the spruce's reaction standards. With 104 photos.

  8. Hispaania nõuab immigrantide vastu võitlemiseks EL-i abi / Anna-Maria Penu

    Penu, Anna-Maria, 1978-


    Üha suureneb Kanaaridele saabuvate Aafrika paadipõgenike hulk, Hispaania asepeaminister Maria Teresa Fernandez de la Vega tahab probleemi lahendamiseks EL-ilt kaasabi. Lisa: Nädalavahetus tõi üle 1000 põgeniku

  9. NATO kõrgeim sõjaväelane: olge abi vastuvõtuks valmis / Raymond Henault ; interv. Raimo Poom

    Henault, Raymond


    NATO sõjalise komitee esimehe sõnul on NATO eesmärk aidata Balti riike õhuturbega kuni 2018. aastani, õhuturbe kontseptsioon vaadatakse üle 2011. aastal. Ämari lennuväli peab muutuma kättesaadavaks kõigile NATO õhuvägedele. Kindral tõstis esile küberkaitse kompetentsikeskuse loomise vajadust ning avaldas arvamust Eesti kaitseväe praeguse mudeli kohta

  10. Kas erivajadustega lapsed saavad õigel ajal abi? / Ene Mägi, Urve Raudsepp-Alt, Ale Sprenk, Peeter Aas


    Küsimusele vastavad: Tallinna Ülikooli Kasvatusteaduste Instituudi eri- ja sotsiaalpedagoogika osakonna juhataja Ene Mägi, Tallinna Haridusameti üldhariduse osakonna peaspetsialist Urve Raudsepp-Alt, Krabi põhikooli direktor Ale Sprenk, Põlva Maavalitsuse haridus-, kultuuri- ja sotsiaalosakonna juhataja Peeter Aas

  11. No consistent effects of ozone exposure for one growth season on levels of polyamines in Picea abies

    Olsen, J.E.; Jensen, E. [Tromsoe Univ. (Norway). Dept. of Plant Physiology and Microbiology


    It is well known that ozone is detrimental to plants, due to its action as a strong oxidant. Polyamines have been suggested as protecting against oxidative stress, and have been reported as being induced by ozone in plants. However, there is a paucity of data on tree species exposed to ozone for an extended period under field-like conditions. In the present investigation, mature spruce clones were exposed to ozone (200 or 400 {mu}g m{sup -3} air) in open-top chambers for an entire growth season at two localities in Norway, and levels of the polyamines putrescine, spermidine and spermine were measured. No consistent effects of ozone fumigation were found on levels of any of the polyamines; not for two clones showing visible injury (yellow needles), and not for four apparently healthy clones. Thus, there was no correlation between sensitivity or tolerance to ozone and polyamine content. However, there were significant differences in polyamine contents between clones and the two localities of exposure 18 refs, 3 figs

  12. Transcriptome Analysis Reveals that Red and Blue Light Regulate Growth and Phytohormone Metabolism in Norway Spruce [Picea abies (L. Karst].

    Fangqun OuYang

    Full Text Available The mechanisms by which different light spectra regulate plant shoot elongation vary, and phytohormones respond differently to such spectrum-associated regulatory effects. Light supplementation can effectively control seedling growth in Norway spruce. However, knowledge of the effective spectrum for promoting growth and phytohormone metabolism in this species is lacking. In this study, 3-year-old Norway spruce clones were illuminated for 12 h after sunset under blue or red light-emitting diode (LED light for 90 d, and stem increments and other growth traits were determined. Endogenous hormone levels and transcriptome differences in the current needles were assessed to identify genes related to the red and blue light regulatory responses. The results showed that the stem increment and gibberellin (GA levels of the seedlings illuminated by red light were 8.6% and 29.0% higher, respectively, than those of the seedlings illuminated by blue light. The indoleacetic acid (IAA level of the seedlings illuminated by red light was 54.6% lower than that of the seedlings illuminated by blue light, and there were no significant differences in abscisic acid (ABA or zeatin riboside [ZR] between the two groups of seedlings. The transcriptome results revealed 58,736,166 and 60,555,192 clean reads for the blue-light- and red-light-illuminated samples, respectively. Illumina sequencing revealed 21,923 unigenes, and 2744 (approximately 93.8% out of 2926 differentially expressed genes (DEGs were found to be upregulated under blue light. The main KEGG classifications of the DEGs were metabolic pathway (29%, biosynthesis of secondary metabolites (20.49% and hormone signal transduction (8.39%. With regard to hormone signal transduction, AUXIN-RESISTANT1 (AUX1, AUX/IAA genes, auxin-inducible genes, and early auxin-responsive genes [(auxin response factor (ARF and small auxin-up RNA (SAUR] were all upregulated under blue light compared with red light, which might have yielded the higher IAA level. DELLA and phytochrome-interacting factor 3 (PIF3, involved in negative GA signaling, were also upregulated under blue light, which may be related to the lower GA level. Light quality also affects endogenous hormones by influencing secondary metabolism. Blue light promoted phenylpropanoid biosynthesis, phenylalanine metabolism, flavonoid biosynthesis and flavone and flavonol biosynthesis, accompanied by upregulation of most of the genes in their pathways. In conclusion, red light may promote stem growth by regulating biosynthesis of GAs, and blue light may promote flavonoid, lignin, phenylpropanoid and some hormones (such as jasmonic acid which were related to plant defense in Norway spruce, which might reduce the primary metabolites available for plant growth.

  13. Testing Romanian seed sources of Norway spruce (Picea abies L. Karst: results on growth traits and survival at age 30

    Marius Budeanu


    Full Text Available Growth traits and survival rate were evaluated in two field trials consisting of 33 provenances (seed stands spread across the entire natural distribution range of Norway spruce in Romania. Total tree height, diameter at breast height (DBH and survival rate were measured at 30 years after planting. Both growth and adaptation traits show substantial genetic variation among the tested seed stands. The amplitude of variation depends markedly on trait and testing site. This fact suggests that the best performing seed stands for growth and adaptation traits at each testing site can be selected. Two groups of valuable populations, located in the North and East of Eastern Carpathians (Apuseni Mountains, were identified. Survival rate was negatively correlated with growth traits, the average values in the two field trials were 68% and 70%. By analyzing growth and adaptation traits together with stem and wood qualitative traits, the best performing populations will be considered as tested seed sources and the forest reproductive material they can provide will be recommended for use in the regions of provenance where the two field trials are located.

  14. Characterization of green seed, an enchancer of abi3-1 in Arabidopsis that affects seed longevity

    Clerkx, E.J.M.; Vries, de M.H.C.; Ruijs, G.J.; Groot, S.P.C.; Koornneef, M.


    Seeds are usually stored in physiological conditions in which they gradually lose their viability and vigor depending on storage conditions, storage time, and genotype. Very little is known about the underlying genetics of seed storability and seed deterioration. We analyzed a mutant in Arabidopsis

  15. Quantitative remote sensing of Norway spruce (Picea abies (L.) Karst.): spectroscopy from needles to crowns to canopies

    Malenovsky, Z.


    Mountain ecosystems represent nearly one fourth of the Earth's land surface, and provide (ecosystem) services to a significant part of the world's human population. As was noted in the 1992 United Nations Conference on Environment and Development (UNCED) inRi

  16. Assessment of sanitary conditions in stands of Norway spruce (Picea abies Karst. damaged by spruce bud scale (Physokermes piceae Schrnk.

    Miezite O


    Full Text Available Spruce bud scale (Physokermes piceae Schrnk. affects tree growth directly and indirectly. Direct injury appears in the form of tissue damage, as insects suck sap from tree phloem. Indirect injury appears as “honeydew”, which results in negative effects on tree growth. Plant sap is saturated with various carbohydrates called photosynthates that are difficult for scaly insects to digest. Therefore it is secreted in excrements, which are subsequently a food source for the black sooty mold (Apiosporium pinophilum Fuckel. The fungus covers needles blocking stomata, causing decreased transpiration and photosynthesis. An inexplicable wither of Norway spruce was reported in Latvia during 2010 due to black sooty mold. However, spruce bud scale was not evident. In 2011, mass propagation of spruce bud scale was observed following the 2010 Norway spruce loss. One objective of this research was to determine if Kraft tree growth classes could be applied to establish the factors responsible for tree foliage damage. Six 21 - 40 year old (second age class Norway spruce stands were evaluated. Two circular sample plots with a 7.98 m radius, and a 200-m2 area were randomly established per each forest stand hectare. Diameter at breast height (dbh, 1.3 m, and height of approximately 30 trees was measured to model a trend. For all trees, Kraft class, and foliage damage level caused by spruce bud scale and black sooty mold were determined. Significant differences were not observed in tree damage levels among stands, however significant differences among damage levels in different Kraft classes were detected (F = 3.45 > Fcrit. = 2.80, α = 0.05 > P = 0.02 found. Overall damage intensity was 29.3 %. Total forestry loss was 1153 LVL (1640 EUR for all surveyed stands (10 ha, and 115 LVL (164 EUR per hectare.

  17. Tallinn lisab abi endistele meeridele ja pensionäridele / Madis Taras, Kerttu Rannamäe

    Taras, Madis


    Tallinna linnavalitsus suurendab toetust pensionäridele, lastega peredele, puudega lastele, endistele Tallinna linnapeadele, samuti suureneb retseptiravimite ja vaktsineerimise osaliseks hüvitamiseks makstav toetus

  18. A forest simulation approach using weighted Voronoi diagrams. An application to Mediterranean fir Abies pinsapo Boiss stands

    Abellanas, B.; Abellanas, M.; Pommerening, A.; Lodares, D.; Cuadros, S.


    Aim of the study. a) To present a new version of the forest simulator Vorest, an individual-based spatially explicit model that uses weighted Voronoi diagrams to simulate the natural dynamics of forest stands with closed canopies. b) To apply the model to the current dynamics of a Grazalema pinsapo stand to identify the nature of its competition regime and the stagnation risks it is currently facing. Area of study: Sierra del Pinar de Grazalema (S Spain) Material and methods: Two large plots representative of Grazalema pinsapo stands were used to fit and validate the model (plus 6 accesory plots to increase the availability of mortality data). Two inventories were carried out in 1998 and 2007 producing tree size and location data. We developed a forest simulator based on three submodels: growth, competition and mortality. The model was fitted, evaluated and validated for Grazalema plots. The simulation outputs were used to infer the expected evolution of structural diversity of forest stands. Main results: Vorest has proved to be a good tool for simulating dynamics of natural closed stands. The application to Grazalema pinsapo stands has allowed assessing the nature of the main processes that are driving its development pathway. We have found that the prevailing size-asymmetric competition dominates the self-thinning process in small-sized trees. At the same time, there is an active tree-size differentiation process. Research highlights: Vorest has proved to be a good tool for simulating natural stands with closed canopies. The Grazalema pinsapo stand under consideration is currently undergoing a natural process of differentiation, avoiding long-term stagnation. (Author)

  19. A forest simulation approach using weighted Voronoi diagrams. An application to Mediterranean fir Abies pinsapo Boiss stands

    Begoña Abellanas


    • The Grazalema pinsapo stand under consideration is currently undergoing a natural process of differentiation, avoiding long-term stagnation. Keywords: Vorest; stand dynamics; individual-based forest model; spatially explicit forest model; pinsapo.

  20. AbiV, a Novel Antiphage Abortive Infection Mechanism on the Chromosome of Lactococcus lactis subsp. cremoris MG1363

    Haaber, Jakob Brandt Borup; Moineau, Sylvain; Fortier, Louis-Charles;


    MG1363. This gene was also found to confer phage resistance to L. lactis MG1363 when it was cloned into an expression vector. A subsequent frameshift mutation in the ORF completely eliminated the phage resistance phenotype, confirming that the ORF was necessary for phage resistance. This ORF provided...

  1. Effects of physical blockage of axial phloem transport on growth of Norway spruce (Picea abies) saplings under drought

    Oberhuber, Walter; Gruber, Andreas; Winkler, Andrea; Lethaus, Gina; Wieser, Gerhard


    Early culmination of maximum radial growth in late spring was found in several coniferous species in a dry inner Alpine environment (Oberhuber et al. 2014). We hypothesized that early decrease in radial stem growth is an adaptation to cope with drought stress, which might require an early switch of carbon allocation to belowground organs. To test this hypothesis we manipulated tree carbon status by physical blockage of phloem transport and soil water availability of Norway spruce saplings (tree height c. 1.5 m) in a common garden experiment to investigate influence of carbon availability and drought on above- and belowground growth. Girdling occurred at different phenological stages during the growing season, i.e., before growth onset, and during earlywood and latewood formation. Non-structural carbohydrates (NSC, soluble sugars and starch) were determined before and after the growing season to evaluate change in tree carbon status. Tree ring analysis revealed that compared to non-girdled controls earlywood width above girdling strikingly increased by c. 170 and 440 %, while latewood width decreased by c. 85 and 55 % in watered and drought stressed trees, respectively. Below girdling no xylem formation was detected. Unexpectedly, preliminary analyses of carbon status revealed striking reduction (c. -80 %) of NSC above and below girdling. Most likely due to reductions in xylem hydraulic conductance, girdling before growth onset reduced leader shoot growth compared to non-girdled controls by c. 45 %, irrespective of water availability. Root dry mass of girdled trees was significantly reduced compared to non-girdled controls (c. 30 % in drought stressed and 45 % in watered trees; p < 0.001). Results suggest that in Norway spruce saplings (1) carbon availability affects radial stem growth, (2) higher basipetal carbon transport occurs under drought supporting our hypothesis of early switch of carbon allocation to belowground when drought stress prevails and (3) minor acropetal transport of carbon from carbon stores in the root system to the stem. We conclude that physical manipulation of carbon availability by disruption of phloem transport is a valuable tool to study relevance of carbon status for tree growth exposed to environmental stress. This study was funded by the Austrian Science Fund (FWF): P25643-B16 "Carbon allocation and growth of Scots pine". Reference Oberhuber W, A Gruber, W Kofler, I Swidrak (2014) Radial stem growth in response to microclimate and soil moisture in a drought-prone mixed coniferous forest at an inner Alpine site. Eur J For Res 133:467-479.

  2. Contrasting carbon allocation responses of juvenile European beech (Fagus sylvatica) and Norway spruce (Picea abies) to competition and ozone.

    Ritter, Wilma; Lehmeier, Christoph Andreas; Winkler, Jana Barbro; Matyssek, Rainer; Edgar Grams, Thorsten Erhard


    Allocation of recent photoassimilates of juvenile beech and spruce in response to twice-ambient ozone (2 × O(3)) and plant competition (i.e. intra vs. inter-specific) was examined in a phytotron study. To this end, we employed continuous (13)CO(2)/(12)CO(2) labeling during late summer and pursued tracer kinetics in CO(2) released from stems. In beech, allocation of recent photoassimilates to stems was significantly lowered under 2 × O(3) and increased in spruce when grown in mixed culture. As total tree biomass was not yet affected by the treatments, C allocation reflected incipient tree responses providing the mechanistic basis for biomass partitioning as observed in longer experiments. Compartmental modeling characterized functional properties of substrate pools supplying respiratory C demand. Respiration of spruce appeared to be exclusively supplied by recent photoassimilates. In beech, older C, putatively located in stem parenchyma cells, was a major source of respiratory substrate, reflecting the fundamental anatomical disparity between angiosperm beech and gymnosperm spruce.

  3. Testing Romanian seed sources of Norway spruce (Picea abies: results on growth traits and survival at age 30

    Marius Budeanu


    Full Text Available Growth traits and survival rate were evaluated in two field trialsconsisting of 33 provenances (seed stands spread across the entire natural distribution range of Norway spruce in Romania. Total tree height, diameter at breast height (DBH and survival rate were measured at 30 years after planting. Both growth and adaptation traits show substantial genetic variation among the tested seed stands. The amplitude of variation depends markedly on trait and testing site. This fact suggests that the best performing seed stands for growth and adaptation traits at each testing site can be selected. Two groups of valuable populations from Romanian Carpathians - the Northern and Western part (Apuseni Mountains - were identified. Survival rate was negatively correlated with growth traits, the average values in the two field trials were 68% and 70%. By analyzing growth and adaptation traits together with stem and wood qualitative traits, the best performing populations will be considered as tested seed sourcesand the forest reproductive material they can provide will be recommended for use in the regions of provenance where the two field trials are located.

  4. Análisis comparativo del "inventario de actitudes y creencias" (ABI) y la "escala de actitudes disfuncionales" (DAS)

    Ruiz Rodríguez, José; Fusté Escolano, Adela


    De acuerdo con los planteamientos de la Terapia Cognitiva (TC) de Aaron Beck y la terapia Racional Emotivo Conductual (TREC) de Albert Ellis, el mantenimiento de creencias rígidas y actitudes disfuncionales constituyen factores de vulnerabilidad a los trastornos psicológicos. En base a estos planteamientos, la adecuada evaluación de las creencias y actitudes disfuncionales es fundamental, no sólo para saber cuáles están en el origen y mantenimiento de la perturbación emocional que puede aquej...

  5. The past ecology of Abies alba provides new perspectives on future responses of silver fir forests to global warming

    Tinner, W.; Colombaroli, D.; Heiri, O.M.; Henne, P.D.; Steinacher, M.; Untenecker, J.; Vescovi, E.; Allen, J.R.M.; Carraro, G.; Conodera, M.; Joos, F.; Lotter, A.F.; Luterbacher, J.; Samartin, S.; Valsecchi, V.


    Paleoecology can provide valuable insights into the ecology of species that complement observation and experiment-based assessments of climate impact dynamics. New paleoecological records (e.g., pollen, macrofossils) from the Italian Peninsula suggest a much wider climatic niche of the important Eur

  6. Estimating the relative nutrient uptake from different soil depths in Quercus robur, Fagus sylvatica and Picea abies

    Göransson, Hans; Wallander, Håkan; Ingerslev, Morten;


    The distribution of fine roots and external ectomycorrhizal mycelium of three species of trees was determined down to a soil depth of 55 cm to estimate the relative nutrient uptake capacity of the trees from different soil layers. In addition, a root bioassay was performed to estimate the nutrient...... or spruce roots was not influenced by soil depth. In modelling the nutrient sustainability of forest soils, the utilization of nutrient resources in deep soil layers has been found to be a key factor. The present study shows that the more shallow-rooted spruce can have a similar capacity to take up...

  7. High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda)

    Wenck, A. R.; Quinn, M.; Whetten, R. W.; Pullman, G.; Sederoff, R.; Brown, C. S. (Principal Investigator)


    Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultivation conditions and different disarmed strains of Agrobacterium to improve transformation. Additional copies of virulence genes were added to three common disarmed strains. These extra virulence genes included either a constitutively active virG or extra copies of virG and virB, both from pTiBo542. In experiments with Norway spruce, we increased transformation efficiencies 1000-fold from initial experiments where little or no transient expression was detected. Over 100 transformed lines expressing the marker gene beta-glucuronidase (GUS) were generated from rapidly dividing embryogenic suspension-cultured cells co-cultivated with Agrobacterium. GUS activity was used to monitor transient expression and to further test lines selected on kanamycin-containing medium. In loblolly pine, transient expression increased 10-fold utilizing modified Agrobacterium strains. Agrobacterium-mediated gene transfer is a useful technique for large-scale generation of transgenic Norway spruce and may prove useful for other conifer species.

  8. Tracing the allocation of recently assimilated C into key metabolites in Norway spruce (Picea abies) shortly after bud break

    Heinrich, Steffen; Dippold, Michaela; Werner, Christiane; Wiesenberg, Guido; Kuzyakov, Yakov; Glaser, Bruno


    Plants allocate carbon (C) to sink tissues depending on phenological, physiological or environmental factors. We still have little knowledge on C partitioning into various cellular compounds and metabolic pathways, especially during tree growth after bud break. Here we investigated C partitioning of freshly assimilated C in Norway spruce by in-situ 13C short-term pulse labeling 15 days after bud break. We quantified 13C incorporation into tree compartments (needles, branches, stem) and into water soluble organic carbon (WSOC) by elemental analyzer-isotope ratio mass spectrometry (EA-IRMS). In addition, we determined 13C allocation into key metabolites of amino acids, hemicellulose sugars, fatty acids and alkanes by compound-specific 13C analysis via gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). The 13C allocation within the trees reflected the needles as major C sink accounting for 86% of the freshly assimilated C. After 6 h 13C was distributed over a broad spectrum of plant metabolites but not homogenously. Highest allocation was observed into structurally relevant compound classes of hemicellulose-derived sugars and proteinogenic amino acids (0.6% and 10% of needle 13C, respectively). However, needle growth also caused high C allocation into pathways not involved in formation of structural compounds like pathways in secondary metabolism, C-1 metabolism or amino acid synthesis from photorespiratory acitivity. C allocation into such pathways could be identified due to the high enrichment of key metabolites within the amino acids. In addition, high 13C allocation was found into the n-alkyl lipid biosynthesis (0.2 % of needle 13C) with 1) higher allocation into intracellular than cuticular fatty acids, presumably for thylakoide membrane formation and 2) decreasing 13C allocation along the lipid transformation and translocation pathways (precursor fatty acids (C16 & C18) > elongated long chain fatty acids > decarbonylated n-alkanes). Consequently, the combination of 13C pulse labeling with compound-specific 13C analysis of key metabolites enabled identification of relevant C allocation pathways during needle growth after bud break. Besides primary metabolism, synthesizing structural cell compounds, a complex network of various pathways consumed the freshly assimilated 13C and kept the majority of the assimilated C in the growing needles.

  9. Incidence of Agrobacterium tumefaciens biovar 1 in and on ‘Paradox’ (Juglans hindsii x Juglans regia) walnut seed collected from commercial nurseries

    The walnut rootstock Paradox (Juglans hindsii (Jeps) Rehder x J. regia L.) is susceptible to Agrobacterium tumefaciens (7) which often results in a high incidence of crown gall in nursery or walnut production orchards. Though A. tumefaciens is susceptible to the commonly used preplant soil fumigant...

  10. 皱叶黄杨的一个新异名%A new synonym of Buxus rugulosa Hatusima



    根据对小叶黄杨(Buxus sinica (Rehder & Wilson) Cheng ex M. Cheng var. parvifolia M. Cheng)模式标本的研究,并比较皱叶黄杨(Buxus rugulosa Hatusima)的标本,而将小叶黄杨作为皱叶黄杨的新异名.

  11. 76 FR 69693 - Tolerance Crop Grouping Program III


    ... pumila; Filbert (hazelnut), Corylus spp.; Hickory nut, Carya spp.; Macadamia nut (bush nut), Macadamia spp.; Pecan, Carya illinoensis; Walnut, black and English (Persian), Juglans spp. EPA proposes to... (Makino) Rehder, J. ailantifolia Carri re) Hickory nut (Carya cathayensis Sarg., C. glabra (Mill.)...

  12. Ühiseid väärtusi hoides : haridusministeerium otsib abi eetikast / Avo Rosenvald ; kommenteerinud Tõnis Lukas, Marju Lauristin ... [jt.


    26. septembril Tallinnas väärtuste ja nende arendamise teemal toimunud konverentsist "Ühiseid väärtusi hoides", mille korraldas Tartu Ülikooli eetikakeskus koostöös Haridus- ja Teadusministeeriumiga

  13. Screening of abscisic acid insensative (ABI) and low phosphorous efficiency (LPE) mutants from some sequenced lines in the sorghum TILLING population

    Sorghum population for Targeting Induced Local Lesion IN Genome (TILLING) was generated from BTx623 in 2005 and publicly available in 2009. After releasing to the public, this population was intensively screened by morphological observation in the field and a number of mutants with useful traits wer...

  14. Keelan, käsen, poon ja lasen või osutan abi? [1.-2. osa] / Kärt Muller

    Muller, Kärt


    Kaalutlusõiguse sisust ja põhimõtetest hoolekande teostamisel väljamõeldud kaasuste näitel, põhjendamiskohustuse sisust ja vajalikkusest. Võrdsest kohtlemisest kaalutlusõiguses, kaalutlusvigade kataloogist ja kaalutlusõiguse teostamisest praktikas Riigikohtu lahendite näite

  15. Estructura espacial y dinámica del bosque montano del sur del Ecuador. Interacciones bióticas y limitaciones abióticas

    Vicuña Merino, Rafael


    Muchos estudios han descrito la composición y diversidad de los bosques montanos tropicales, pero los patrones espaciales y las diferentes tipos de relaciones de estos, entre especies o entre grupos funcionales ha sido poco documentada. El presente trabajo se realizó en tres parcelas completamente censadas del bosque de la Estación Biológica “Chamusquin” (Zamora Chinchipe, Ecuador), y se plantea como objetivo principal conocer la estructura espacial y dinámica del bosque montano tropical del ...

  16. Defence reactions of mature Norway spruce (Picea abies before and after inoculation of the blue-stain fungus Endoconidiophora polonica in a drought stress experiment

    Netherer Sigrid


    Full Text Available We performed an inoculation experiment using the blue-stain fungus Endoconidiophora polonica at the Rosalia Roof study site, which was set up to study drought effects on Norway spruce susceptibility to attacks by the European spruce bark beetle Ips typographus. Tree resistance parameters such as resin flow rate and extent of hypersensitive wound reaction in the phloem were investigated prior to and after fungal infection. Sample trees with different drought treatments (trees fully covered or semi-covered by roofs, control trees were inoculated with E. polonica in July 2014. In order to check for areas of phloem necrosis, the outer bark around the inoculation holes was scratched off 6 weeks later. We recorded the amount of resin exudation within 12 hours overnight in August and September 2013 and 2014. Although wound reaction zones did not differ in size between tree collectives of the various treatments, fully covered trees tended to exhibit larger necrotic areas compared to control trees. The least water supplied trees showed lowest resin flow rates prior to fungal inoculation, but were the only group to show significantly enhanced resin flow five weeks after the evaluation of defence reactions. Our results agree with earlier findings that wounding and few fungal inoculations can increase tree resistance in the medium term given not too severe water stress. Further investigations will clarify how water stress affects the availability of non-structural carbohydrates for secondary metabolism, when depletion of resources eventually occurs, and at which point trees are most susceptible to bark beetle attack.

  17. The Dynamics and Variability of Radial Growth in Provenance Trials of Norway Spruce (Picea abies (L. Karst. Within and Beyond the Hot Margins of its Natural Range

    Neculae ŞOFLETEA


    Full Text Available Multi-site field trials provide valuable data for the investigation of possible effects of environmental changes on forest tree species. We analyze the descendants of plus trees from 33 Norway spruce seed sources of Romanian Carpathians, at age 30, in four comparative field trials: two established in the natural range of species and two outside of it. The dynamics and variation of radial growth, earlywood and latewood were analyzed. The influence of populations, site conditions and climatic factors were also quantified. The provenances response in the four comparative trials was asymmetric for mean radial growth, but its dynamics was less favourable outside of the natural range. Analysis of variance showed significant differences (P<0.001 between the testing sites, but non-significant (P<0.05 for the populations. Populations x localities interaction was high (P<0.001 in the first half of the testing period, but decreased over time, becoming not significant at the age of 30. At intra-populational level, the average coefficient of variation for radial growth was higher outside the natural range, whereas the proportion of latewood decreased. The temperature in the first half of the growing season negatively influenced (P<0.001 the radial increment, but in the latewood proportion significat effects (P˂0.05 were recorded only in trials located outside of the natural range. An increased variability of radial growth and a decrease of latewood proportion are expected in Norway spruce stands located at lower altitudes, towards the limits of the natural range. Our results may contribute to the sustainable management of Norway spruce forests within and outside its natural range.

  18. Biomass Conversion and Expansion Factors for Young Norway Spruce (Picea abies (L. Karst. Trees Planted on Non-Forest Lands in Eastern Carpathians

    Ioan DUTCA


    Full Text Available In this study biomass conversion and expansion factors (BCEFs were developed for young Norway spruce trees planted on non-forest lands, in order to support quantification of carbon stock changes in biomass pools of afforestation works. Regression models for stem volume and stem wood density were also developed. The data set included 250 trees collected from 25 plantations between 1 and 12 years old, located in the Eastern Carpathians of Romania. The study shows that BCEFs decreased with increasing tree dimensions, following an exponential trend. In all proposed models the highest prediction was reached when both variables considered (i.e. root-collar diameter and height were used together. However, used separately, height produced a slightly higher prediction compared to root-collar diameter. Stem volume was well predicted by both root-collar diameter and height. Anyway, a significant improvement in prediction resulted when both variables were used together. Stem wood density decreased sharply with the increase of the two tree dimensions used as variables.

  19. On Fuzzy Regular-I-Closed Sets, Fuzzy Semi-I-Regular Sets, Fuzzy ABI-Sets and Decompositions of Fuzzy Regular-I-Continuity, Fuzzy AI - Continuity

    Yildiz, Cemil; ABBAS, Fadhil


     The concepts of fuzzy regular-I-closed set and fuzzy semi-I-regular set in fuzzy ideal topological spaces are investigated and some of their properties are obtained. Key words: Topological, Spaces, Fuzzy, Regular, Sets

  20. Leaching versus input of nitrogen, potassium and magnesium in different fertilizer regimens in Christmas tree stands of Abies nordmanniana in Denmark

    Pedersen, Lars Bo; Christensen, Claus Jerram; Ingerslev, Morten


    The effects of nitrogen (N), potassium (K) and magnesium (Mg) fertilization and deposition on nutrient leaching were assessed in four Danish Nordmarm fir Christmas tree stands on former arable land. NPK 23-3-7 fertilizer type was applied in doses between 0 and 1200 kg ha(-1) year(-1). Deposition...

  1. Short-term effects of whole-tree and stem-only harvesting on C and N fluxes in two Picea abies stands, Norway

    Janne Kjønaas, O.; Clarke, Nicholas; Eldhuset, Toril; Hietala, Ari; Cross, Hugh; Holt Hanssen, Kjersti; Økland, Tonje; Lange, Holger; Frode Nordbakken, Jørn; Røsberg, Ingvald


    Tree harvest and different harvesting methods may affect the soil carbon (C) pool in forest ecosystems. In conventional stem-only timber harvesting (SOH), branches and tops that are left in the forests may contribute to the build-up of the soil carbon pool. In whole-tree harvesting (WTH), inputs of organic matter from branches and tops are strongly reduced. We established field experiments at Gaupen, SE and Vindberg, SW Norway, to study the short-term effects of SOH and WTH on processes affecting the accumulation and loss of soil C. Logging residues on the WTH plots were collected in piles that were removed after 6 months, rendering two sub treatments (WTH-pile and WTH-removal areas). We weighed selected trees and logging residues, surveyed understorey biomass production, quantified pre-harvest soil C and nutrient pools down to 30 cm. Soil respiration was measured and soil water sampled monthly during the growing season, while temperature and moisture were measured continuously. Organic and mineral horizons were incubated at different temperatures to estimate potential C and N mineralization, and deep sequencing of the ITS2 barcode region of fungal DNA was performed on the samples. Litterbags were deployed in the SOH plots. The logging residues amounted to 2.2-2.4 kg C m-2. At Gaupen, the mean in situ soil respiration rates increased following harvest with all treatments, but were significantly higher in WTH-pile and SOH relative to the WTH-removal areas in the first year as well as the fourth year of treatment. The former rates included aboveground decomposing needles and twigs but excluded coarser branches. The observed increase in the WTH-removal areas may be related to decomposing roots, as well as to increased C mineralization partly due to the higher soil temperatures following harvest. Soil temperature was the single most important factor explaining the variability in soil respiration rates over all treatments. At Vindberg, a decrease in soil respiration was observed with all treatments in the second and third years following harvest. At both sites, decomposition of logging residues from needles was more rapid relative to twigs and fine roots. The decomposing residues released a substantial amount of nitrogen which was gradually reflected in the soil water at 30 cm soil depth. A considerable increase in the NO3-N concentration also in the WTH-removal areas in the second year following harvest suggests an increase in N availability from decomposing fine roots and/or soil organic matter. The increased N availability in the WTH-removal areas was supported by results from short term lab incubations of undisturbed soil from the forest floor. The changes in the WTH-removal areas were also reflected in the soil fungal diversity: saprophytic ascomycetes on decaying plant material showed a striking increase in all treatments. For the WTH-removal areas, this may, again, be related to the increased input of root litter; however, the decrease in mycorrhizal basidiomycete species and the vigorous increase of ascomycetes following harvest may also affect the C mineralization of soil organic matter.

  2. Heterobasidion annosum root and butt rot of Norway spruce, Picea abies: Colonization by the fungus and its impact on tree growth

    Bendz-Hellgren, M. [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Mycology and Pathology


    Diameter growth losses associated with decay were quantified on a nationwide scale, and volume growth losses were measured in two stands. Diameter growth losses were 8-10% during a 5-year period in the nationwide study and 23% in one of the stands, whereas in the other stand, no volume losses could be attributed to decay. The effects of stump moisture content, temperature and time elapsed between felling and inoculation on the establishment of H. annosum spore infections in stumps were investigated among stumps resulting from thinnings and clear-cuttings. Furthermore, inoculations with H. annosum conidia were made between 0 hours and 4 weeks after thinning. The incidence of stump infections was lower on clear-cut areas than in thinned stands, but high enough to warrant stump treatment on clear-cuttings. A positive relation was found between heartwood moisture content and the proportion of heartwood infected, whereas the opposite relation was found for sapwood. The establishment of new conidiospore infections decreased with time, and it appeared that stumps were no longer susceptible to infection after 3 weeks had elapsed since felling. Roots of stumps and trees on forest land or former arable land were inoculated with H. annosum treated sawdust. The growth rate of H. annosum in roots of stumps was 25 cm/year, corresponding to 2.5 to 3 times the growth rate in tree roots. Previous land use did not affect the fungal rate of spread. Also, the average initial spread rate of H. annosum in naturally infected Norway spruce stems was estimated at 30 cm/year 156 refs, 9 figs

  3. Time consumption and productivity of skidding Silver fir (Abies alba Mill. round wood in reduced accessibility conditions: a case study in windthrow salvage logging form Romanian Carpathians

    Stelian Alexandru Borz


    Full Text Available Natural calamities (especially windthrows may generate difficult work conditions in timber harvesting operations. When associated with the reduced accessibility conditions, the work conditions become even harder. This study investigates the time consumptions on specific work elements in timber skidding, develops time prediction models for timber skidding work elements and assesses the production rates for timber skidding in reduced accessibility stands where windthrow salvage cuttings were applied. Following a time study done for two skidders (TAF 690 OP and TAF 657 operating simultaneously in the same felling area, it has been found that, in average, in a delay free skidding cycle time, lateral winching accounted for a share of 26-33%, on-trail skidding accounted for a share of 64-71% and landing operations accounted for a share of 3%.Total delays accounted for 51% and 43% of the total work time in the case of TAF 690 OP skidder and TAF 657skidder respectively. Regression models for lateral winching (both skidders revealed that the winching distance and the number of logs were the relevant predictors for the time consumption estimation (p<0.01, whereas in the case of on-trail skidding only the skidding distance was relevant (p<0.01. For the overall skidding operation (excluding landing operations winching and skidding distances were found as relevant predictors (p<0.01 in case of TAF 690 OP, whereas the number of logs became an additional relevant predictor in case of TAF 657.In conditions of an average winching distance of 19.90 m and an average on-trail skidding distance of 980.32 m, the time study yielded a net production rate of 7.70 m3h-1 and a gross production rate of 3.75 m3h-1 in the case of TAF 690 OP skidder. By comparison, in the case of TAF 657 skidder, for an average winching distance of 22.86 m and an average on-trail skidding distance of 871.00 m, the net and gross production rates were of 5.61 m3h-1 and 3.20 m3h-1 respectively.

  4. Species Favourability Shift in Europe due to Climate Change: A Case Study for Fagus sylvatica L. and Picea abies (L. Karst. Based on an Ensemble of Climate Models

    Wolfgang Falk


    Full Text Available Climate is the main environmental driver determining the spatial distribution of most tree species at the continental scale. We investigated the distribution change of European beech and Norway spruce due to climate change. We applied a species distribution model (SDM, driven by an ensemble of 21 regional climate models in order to study the shift of the favourability distribution of these species. SDMs were parameterized for 1971–2000, as well as 2021–2050 and 2071–2100 using the SRES scenario A1B and three physiological meaningful climate variables. Growing degree sum and precipitation sum were calculated for the growing season on a basis of daily data. Results show a general north-eastern and altitudinal shift in climatological favourability for both species, although the shift is more marked for spruce. The gain of new favourable sites in the north or in the Alps is stronger for beech compared to spruce. Uncertainty is expressed as the variance of the averaged maps and with a density function. Uncertainty in species distribution increases over time. This study demonstrates the importance of data ensembles and shows how to deal with different outcomes in order to improve impact studies by showing uncertainty of the resulting maps.

  5. Sušenje obične jele (Abies alba Mill.) s proizvodnog stajališta na primjeru nekolicine bukovo-jelovih sastojina

    Paladinić, Elvis; Štimac, Denis; Marjanović, Hrvoje; Balenović, Ivan; Ostrogović, Maša Zorana


    Problematika koju rad istražuje je sušenje obične jele u bukovo-jelovim šumama s proizvodnog stajališta. Proizvodnjom šumskih drvnih sortimenata jele u sastojinama različitih kategorija zdravstvenog stanja, ostvaruje se sortimentna struktura koja vrlo često značajno odstupa od planirane strukture, a time i od planiranih prihoda proizvodnje. Navedene razlike između planirane i ostvarene proizvodnje, koje su značajne za sastojine u kojima je jela umjereno do izrazito narušenog zdravstvenog stan...

  6. Comparison of VOC emissions between air-dried and heat-treated Norway spruce ( Picea abies), Scots pine ( Pinus sylvesteris) and European aspen ( Populus tremula) wood

    Hyttinen, Marko; Masalin-Weijo, Marika; Kalliokoski, Pentti; Pasanen, Pertti


    Heat-treated wood is an increasingly popular decoration material. Heat-treatment improves dimensional stability of the wood and also prevents rot fungus growth. Although production of heat-treated wood has been rapidly increasing, there is only little information about the VOC emissions of heat-treated wood and its possible influences on indoor air quality. In the present study, VOC emissions from three untreated (air-dried) and heat-treated wood species were compared during a four weeks test period. It appeared that different wood species had clearly different VOC emission profiles. Heat-treatment was found to decrease VOC emissions significantly and change their composition. Especially, emissions of terpenes decreased from softwood samples and aldehydes from European aspen samples. Emissions of total aldehydes and organic acids were at the same level or slightly higher from heat treated than air-dried softwood samples. In agreement with another recent study, the emissions of furfural were found to increase and those of hexanal to decrease from all the wood species investigated. In contrast to air-dried wood samples, emissions of VOCs were almost in steady state from heat treated wood samples even in the beginning of the test.

  7. Effects of forest canopy gap on biomass of Abies faxoniana seedlings and its allocation in subalpine coniferous forests of western Sichuan

    Junren XIAN; Tingxing HU; Yuanbin ZHANG; Kaiyun WANG


    Using a strip transect sampling method, the density, height (≤ 100 cm), basal diameter and compo-nents of biomass of Abiesfaxoniana seedlings, living in a forest gap (FG) and under the forest canopy (FC) of sub-alpine natural coniferous forests in western Sichuan, were investigated and the relationships among different com-ponents of biomass analyzed. The results indicated that the density and average height (H) of A. faxoniana seed-lings were significantly different in the FG and under the FC, with the values being 12903 and 2017 per hm2, and 26.6 and 24.3 cm. No significant differences were found in the average basal diameter (D) and biomass. The biomass allocation in seedling components was significantly affec-ted by forest gap. In the FG, the biomass ratio of branch to stem reached a maximum of 1.54 at age 12 and then declined and fluctuated around 0.69. Under the FC, the biomass ratio of branch to stem increased with seedling growth and exceeded 1.0 at about age 15. The total bio-mass and the biomass of leaves, stems, shoots and roots grown in the FG and under the FC were significantly correlated with D2H. There were significant and positive correlations among the biomass of different components.

  8. Moderate effects of reforestation with Norway spruce (Picea abies) on carbon storage and turnover in a Swiss sub-alpine pasture

    Hiltbrunner, D.; Hagedorn, F.; Niklaus, P. A.; Zimmermann, S.; Schmidt, M. W. I.


    In alpine regions the forested area is strongly increasing through woody plant encroachment on abandoned pastures or by man-made afforestations. These natural or artificial reforestations, in fact, have several implications on the nutrient cycling between plants and soils and thus, are likely to affect carbon turnover. Although afforestations are to be accounted as a sink according to the Kyoto protocol, there are still uncertainties about their effects on the soil carbon storage. In the present study, we assessed soils under pasture, an adjacent chronosequence of spruce afforestations (25-45 years) and a mature spruce forest (older than 120 years) on a homogenous slope in a Swiss sub-alpine ecosystem. While the soil bulk densities were not affected by the land use change, carbon concentrations in the mineral soil decreased 25-45 years after tree establishment. However, no differences between pasture and the mature forest were apparent, indicating that the C-loss after land use conversion was only transient. Up to 2.5kg m-2 C was additionally stored in the organic layer of the oldest stands, resulting in a net C gain in the old forest soils. C:N-ratios of the soil organic matter (SOM) considerably increased with stand age in the uppermost soil layer, displaying the distinct chemical composition of the plant input. In accordance, a shift of the δ13C natural abundance of the SOM in the uppermost mineral layer towards a less negative signal was observed with tree development. The abundance of soil microorganisms, as identified by their phospholipid fatty acids (PLFA), was only moderately affected by vegetation type in the mineral soils. In contrast, a strong alteration of the microbial community composition with a decreasing proportion of fungi from the organic layers to the uppermost mineral layer was observable. Our results show that afforestation with spruce trees on an extensively used sub-alpine pasture only led to a transient loss of C in the mineral soils. In contrast, the accumulation of additional C in the organic layer resulted in higher C-stocks in the old forest as compared to the pasture. Therefore, afforestation with coniferous trees is likely to increase the total amount of C stored, particularly if also the plant biomass is taken into account.

  9. Elevated CO{sub 2} and development of frost hardiness in Norway spruce (picea abies (L.) Karst.); Oekt CO{sub 2} og utvikling av frostherdighet i gran

    Dalen, Lars Sandved


    This thesis discusses controlled laboratory experiments carried out to study the effects of CO{sub 2} pollution on Norwegian spruce. It was found that elevated CO{sub 2} increased height growth and biomass production. It slightly increased frost hardiness, but only at high nitrogen values. There was no evidence of adverse effects of elevated CO{sub 2} on the phenology of bud set and the development of frost hardiness. Although not statistically significant, there seemed to be a consistently higher concentration of soluble carbohydrates in one-season-old Norway spruce seedlings treated with elevated CO{sub 2}. This was not found in three-year-old seedlings grown in open top chambers, possibly indicating a down-regulation of photosynthesis or a transition from free to predetermined growth, and change in allocation of photosynthates with age. Treatment with high or low concentrations of CO{sub 2} and nitrogen fertilizer did not affect apoplastic chitinolytic activity during cold acclimation, nor were there any effects on antifreeze activity in these apoplastic extracts from cold acclimated needles. 149 refs., 21 figs., 8 tabs.

  10. Predictors and dietary consequences of frequent intake of high-sugar, low-nutrient foods in 1-year-old children participating in the ABIS study.

    Brekke, Hilde K; van Odijk, Jenny; Ludvigsson, Johnny


    Foods rich in sugar have been suggested to contribute to the increasing prevalence of obesity in children. The aim of this report is to investigate the dietary pattern in 1-year-old children who frequently receive foods rich in sugar but low in nutrients and to study associated demographic and parental factors. During 1977-9, 21,700 infants were invited to participate in this prospective, population-based, longitudinal cohort study. Screening questionnaires were completed for 16,070 infants after delivery. Follow-up questionnaires from 10,762 children at 1 year of age are included in the analysis. It was found that 24% of the children received sweets/pastries more often than one or two times per week. They had a higher intake of French fries, potato crisps and cream as well as a lower intake of fruit and vegetables. A frequent intake of sugar-rich, low-nutrient foods was significantly associated with several maternal factors (high intake of sweets/pastries during pregnancy, young age, mother living alone) as well as presence of older siblings. Maternal smoking during pregnancy and maternal overweight were of borderline significance. Parental education level was inversely associated with the frequency of intake of sweets/pastries in the child. Children who frequently receive sweets/pastries also have an otherwise unfavourable dietary pattern. Several parental and demographic factors were associated with this feeding pattern, especially high intake of sweets/pastries during pregnancy. Screening of pregnant women for risk predictors like consumption of sweets/pastries, young age and smoking could be possible ways of identifying children at future risk for low dietary quality.

  11. Impact of traffic on δ15N, δ13C and δ18O of needles and annual tree rings of Norway spruce (Picea abies L.

    Guerrieri MR


    Full Text Available Increase of nitrogen depositions, as oxidized (NOx and reduced (NHx compounds, has important implications on ecosystem nitrogen cycle. NOx comes predominantly from fossil fuel combustion in car engines and industrial processes, while agricultural activities (cattle farming, fertilizations are the main sources for NHx emissions. Such fertilisations could stimulate growth and, therefore, productivity of forests, especially in temperate forests, where nitrogen is a limiting factor. On the other hand an excess of nitrogen can lead to an acidification of the soil and have a negative impact on the microbial fauna and structure of plants. NOx and NHx depositions can be separated with the help of stable isotopes with different 15N-values reported for NOx originating from combustion sources. Consequently it was observed that the nitrogen isotopic composition of the vegetation reflects the isotopic signature of nitrogen sources. Our preliminary results on needles of Norway spruce trees exposed to NOx emissions along a transect perpendicular to a highway close to Faido and Brittnau show a clear isotopic enrichment in 15N in trees growing closer to traffic pollution, indicating an uptake of atmospheric nitrogen by stomatal pathway. Carbon and oxygen isotopic composition measured in tree rings reveals the physiological response in plants. Trees more exposed to air pollution from traffic show an increase in δ13C and δ18O in tree rings, since mid sixties next to Erstfeld. This could mean a higher photosynthetic activity, enhanced by NOx traffic emissions, under low or not changed stomatal conductance. Our results confirm that stable isotopes of carbon, oxygen and nitrogen measured in needles and tree rings represent an important tool to monitor the impact of NOx load on tree physiology.

  12. Whole-tree seasonal nitrogen uptake and partitioning in adult Fagus sylvatica L. and Picea abies L. [Karst.] trees exposed to elevated ground-level ozone.

    Weigt, R B; Häberle, K H; Rötzer, T; Matyssek, R


    The effect of long-term exposure of twice-ambient O(3) (2 × O(3)) on whole-tree nitrogen (N) uptake and partitioning of adult beech and spruce was studied in a mixed forest stand, SE-Germany. N uptake as (15)N tracer and N pools were calculated using N concentrations and biomass of tree compartments. Whole-tree N uptake tended to be lower under 2 × O(3) in both species compared to trees under ambient O(3) (1 × O(3)). Internal partitioning in beech showed significantly higher allocation of new N to roots, with mycorrhizal root tips and fine roots together receiving about 17% of new N (2 × O(3)) versus 7% (1 × O(3)). Conversely, in spruce, N allocation to roots was decreased under 2 × O(3). These contrasting effects on belowground N partitioning and pool sizes, being largely consistent with the pattern of N concentrations, suggest enhanced N demand and consumption of stored N with higher relevance for tree-internal N cycling in beech than in spruce.

  13. Below-ground effects of enhanced tropospheric ozone and drought in a beech/spruce forest (Fagus sylvatica L. / Picea abies [L.] Karst)

    The effects of experimentally elevated O3 on soil respiration rates, standing fine-root biomass, fine-root production and δ13C signature of newly produced fine roots were investigated in an adult European beech/Norway spruce forest in Germany during two subsequent years with cont...

  14. Detection, quantification and modelling of small-scale lateral translocation of throughfall in tree crowns of European beech (Fagus sylvatica L.) and Norway spruce (Picea abies (L.) Karst.)

    Frischbier, Nico; Wagner, Sven


    The redistribution of precipitation in forests depends on the amount of above-canopy precipitation and is characterised by high small-scale variability. Although higher and lower values of net forest precipitation at small scales are typically averaged at larger spatial scales, the small-scale variability of throughfall needs to be understood because subordinate ecological processes in the forest ecosystem, e.g., regeneration of tree species, often take place at the same small scale. High stemflow amounts and canopy driplines at the crown edge of particular tree species can only be explained by lateral flow processes within tree crowns. This study tests the hypothesis that lateral water translocation within the crown can be determined from simultaneous records of precipitation at defined measurement points below and above the canopy by taking single-tree characteristics such as species and crown width into account. Spatially explicit simultaneous measurements of gross precipitation (above-canopy reference) and throughfall were conducted repeatedly at 175 measurements points in a mixed European beech-Norway spruce stand for a total of 26 individual rain events. Subsequent analysis with a new regression approach resulted in an estimated average canopy storage capacity of 3.5 mm and 5.8 mm for beech (leaf-bearing period) and spruce stands, respectively. Values of calculated lateral flow showed considerable variability between individual measurement points. The highest discharge amounts were observed at positions below the inner beech crowns during the leaf-bearing period. For an exemplary rainfall event with a gross precipitation of 25 mm, the predicted discharge ranged from 5 mm underneath the inner beech crown to about zero near the crown edge. A comparison with the measured values indicated that the predicted amount of lateral flow, which could be translated into stemflow for single beech trees, was realistic. However, for the same rainfall event, lateral flow in spruce crowns was mainly identified in the outer crown. The derived functions for calculating lateral water translocation may be incorporated into single-tree models.

  15. Belowground effects of enhanced tropospheric ozone and drought in a beech/spruce forest (Fagus sylvatica L./Picea abies [L.] Karst)

    Nikolova, Petia S., E-mail: nikolova@wzw.tum.d [Ecophysiology of Plants, Technische Universitaet Muenchen, Weihenstephan Center of Life and Food Sciences, Am Hochanger 13, 85354 Freising (Germany); Andersen, Christian P. [Western Ecology Division, National Health and Environmental Effects Research Laboratory, United States Environmental Protection Agency, 200 SW 35th St., Corvallis, OR 97333 (United States); Blaschke, Helmut; Matyssek, Rainer; Haeberle, Karl-Heinz [Ecophysiology of Plants, Technische Universitaet Muenchen, Weihenstephan Center of Life and Food Sciences, Am Hochanger 13, 85354 Freising (Germany)


    The effects of experimentally elevated O{sub 3} on soil respiration rates, standing fine-root biomass, fine-root production and delta{sup 13}C signature of newly produced fine roots were investigated in an adult European beech/Norway spruce forest in Germany during two subsequent years with contrasting rainfall patterns. During humid 2002, soil respiration rate was enhanced under elevated O{sub 3} under beech and spruce, and was related to O{sub 3}-stimulated fine-root production only in beech. During dry 2003, the stimulating effect of O{sub 3} on soil respiration rate vanished under spruce, which was correlated with decreased fine-root production in spruce under drought, irrespective of the O{sub 3} regime. delta{sup 13}C signature of newly formed fine-roots was consistent with the differing g{sub s} of beech and spruce, and indicated stomatal limitation by O{sub 3} in beech and by drought in spruce. Our study showed that drought can override the stimulating O{sub 3} effects on fine-root dynamics and soil respiration in mature beech and spruce forests. - Drought has the capacity to override the stimulating ozone effect on soil respiration in adult European beech/Norway spruce forest.

  16. Phytohormones in needles of spruce (Picea abies [L.] Karst.) under different levels of air pollution in the open-top chamber experiment at Edelmannshof

    Christmann, A.; Frenzel, B. [Hohenheim Univ., Stuttgart (Germany). Inst. fuer Botanik


    The plant hormones ethylene (ACC, MACC), abscisic acid and indoleacetic acid were investigated between August 1988 and December 1989 in current-year and one-year-old needles of the twelve spruce trees of the Edelmannshof experiment. Data from this period do not allow to reliably differentiate between consequences of the reduced impact of immissions (open-top chambers receiving charcoal-filtered air) and individual differences of the trees investigated. The conditions are discussed that might have made such a differentiation possible but which were not fulfilled at Edelmannshof. (orig.)

  17. Estrogenic activity of 7-hydroxymatairesinol potassium acetate (HMR/lignan) from Norway spruce (Picea abies) knots and of its active metabolite enterolactone in MCF-7 cells.

    Cosentino, Marco; Marino, Franca; Ferrari, Marco; Rasini, Emanuela; Bombelli, Raffaella; Luini, Alessandra; Legnaro, Massimiliano; Delle Canne, Marco Gioacchino; Luzzani, Marcello; Crema, Francesca; Paracchini, Silvano; Lecchini, Sergio


    Lignans are plant polyphenols which may possess anticancer, antioxidant, antimicrobial, anti-inflammatory and immunomodulatory activities. In particular, the lignan 7-hydroxymatairesinol (HMR/lignan, HMR) is a novel precursor of the mammalian lignan enterolactone (EL). In the present study, we investigated the estrogenicity of HMR and of EL in comparison to estradiol (E2), by measuring their effects on growth and apoptotic markers in the human estrogen-sensitive cell line MCF-7. HMR, EL and E2 concentration-dependently increased the percentage of MCF-7 cells in the S phase of the cell cycle, with the following relative potencies: E2 congruent with EL>HMR, and efficacies: E2>HMR>EL. Treatment of MCF-7 cells with either HMR, EL or E2 also increased the Bcl-2/Bax mRNA ratio. The effects of HMR and EL were reduced in the presence of the estrogen receptor (ER) antagonist tamoxifene. We conclude that both HMR and its metabolite EL are endowed with estrogenic activity, which is likely to be exerted through the contribution of ER-dependent pathways and to target the same intracellular mechanisms acted upon by E2. The estrogenicity of HMR and EL is however milder than that of E2, as indicated by the lower potencies and efficacies of both lignans. The present results support the notion that dietary supplementation with HMR may result in a mild estrogenic activity, both directly and by providing a suitable source for endogenous EL.

  18. Body Dissatisfaction Measured with a Figure Preference Task and Self-Esteem in 8 Year Old Children - a Study within the ABIS-Project

    A. Sepa


    Full Text Available Body dissatisfaction has been related to low self-esteem and depression in adolescents. With regard to the current world wide rise in childhood obesity and common stigmatization of adults and children with obesity, easy to use and cost effective measurements of body dissatisfaction would be helpful in epidemiological research. In the current study, detailed data on body measurements with regard to perceived and ideal body size and body dissatisfaction, as measured with the figure preference task, are presented for a population based sample of 3837 children. Perceived body size correlations to weight, body mass index [BMI], and waist circumference were between 0.41 and 0.54; and to height between 0.12 and 0.21. Odds ratios for lower self-esteem increased with increase in body dissatisfaction. Gender differences in body dissatisfaction were present but not found in relation to self-esteem. It is concluded that the figure preference task yields valuable information in epidemiological studies of children as young as 7.5 years of age. It is argued, that the figure preference task is an additional measurement which theoretically relates to psychological stress in childhood.

  19. Noteworthy records of Hispines from Belize (Coleoptera: Chrysomelidae)

    Robert F C Naczi; Staines, Charles L.


    Cephaloleia consanguinea Baly, Cephaloleia fulvolimbata Baly, Cephaloleia ruficollis Baly, Chalepus amabilis Baly, Chalepus brevicornis (Baly), Chalepus pici Descarpentries and Villiers, Microrhopala erebus (Newman), Octhispa bimaculata Uhmann, Octotoma championi Baly, Pseudispa tuberculata Staines, Sceloenopla erudita (Baly), Stenispa guatemalensis Uhmann, Sumitrosis gestroi (Weise), and Sumitrosis terminatus (Baly) (Coleoptera: Chrysomelidae: Cassidinae) are new country records of hispine c...

  20. Two additions to the Mexican orchid flora Dos adiciones a la orquideoflora mexicana

    Gerardo A. Salazar


    Full Text Available Two species of Orchidaceae are recorded here for the first time in Mexico, namely Cryptarrhena guatemalensis and Domingoa gemma. Cryptarrhena guatemalensis is widespread in the Neotropics and can be distinguished from C. lunata, the only other species of the genus, by possessing pseudobulbs and narrowly triangular, attenuate apical labellum lobules. Domingoa gemma, previously known only from Guatemala and El Salvador, differs from the similar D. purpurea in the smooth, linear-lanceolate leaves and labellum lacking a prominent sac at the base. Both species are known in Mexico from single localities in the state of Chiapas.Se registran aquí por primera vez en México 2 especies de Orchidaceae, Cryptarrhena guatemalensis y Domingoa gemma. Cryptarrhena guatemalensis está ampliamente distribuida en el neotrópico y se distingue de C. lunata, la única otra especie del género, por presentar pseudobulbos y por los lóbulos apicales del labelo angostamente triangularesy atenuados. Domingoa gemma, previamente conocida sólo de Guatemala y El Salvador, difiere de D. purpurea, especie similar, en las hojas lisas, linear-lanceoladas y el labelo sin un saco prominente en la base. En México sólo se conoce una localidad para cada especie y ambas se ubican en el estado de Chiapas.

  1. NCBI nr-aa BLAST: CBRC-LAFR-01-3534 [SEVENS

    Full Text Available CBRC-LAFR-01-3534 gb|ABI14027.1| luteinizing hormone receptor [Bubalus bubalis] gb|...ABI14028.1| luteinizing hormone receptor [Bubalus bubalis] gb|ABI14029.1| luteinizing hormone receptor [Bubalus bubali...s] gb|ABI14030.1| luteinizing hormone receptor [Bubalus bubalis] gb|ABI14031.1| luteinizing hormone receptor [Bubalus bubalis] ABI14027.1 1e-106 89% ...

  2. NCBI nr-aa BLAST: CBRC-EEUR-01-1181 [SEVENS

    Full Text Available CBRC-EEUR-01-1181 gb|ABI14027.1| luteinizing hormone receptor [Bubalus bubalis] gb|...ABI14028.1| luteinizing hormone receptor [Bubalus bubalis] gb|ABI14029.1| luteinizing hormone receptor [Bubalus bubali...s] gb|ABI14030.1| luteinizing hormone receptor [Bubalus bubalis] gb|ABI14031.1| luteinizing hormone receptor [Bubalus bubalis] ABI14027.1 1e-154 86% ...

  3. NCBI nr-aa BLAST: CBRC-DNOV-01-0593 [SEVENS

    Full Text Available CBRC-DNOV-01-0593 gb|ABI14027.1| luteinizing hormone receptor [Bubalus bubalis] gb|...ABI14028.1| luteinizing hormone receptor [Bubalus bubalis] gb|ABI14029.1| luteinizing hormone receptor [Bubalus bubali...s] gb|ABI14030.1| luteinizing hormone receptor [Bubalus bubalis] gb|ABI14031.1| luteinizing hormone receptor [Bubalus bubalis] ABI14027.1 1e-168 90% ...

  4. A widespread bacteriophage abortive infection system functions through a Type IV toxin-antitoxin mechanism.

    Dy, Ron L; Przybilski, Rita; Semeijn, Koen; Salmond, George P C; Fineran, Peter C


    Bacterial abortive infection (Abi) systems are 'altruistic' cell death systems that are activated by phage infection and limit viral replication, thereby providing protection to the bacterial population. Here, we have used a novel approach of screening Abi systems as a tool to identify and characterize toxin-antitoxin (TA)-acting Abi systems. We show that AbiE systems are encoded by bicistronic operons and function via a non-interacting (Type IV) bacteriostatic TA mechanism. The abiE operon was negatively autoregulated by the antitoxin, AbiEi, a member of a widespread family of putative transcriptional regulators. AbiEi has an N-terminal winged-helix-turn-helix domain that is required for repression of abiE transcription, and an uncharacterized bi-functional C-terminal domain, which is necessary for transcriptional repression and sufficient for toxin neutralization. The cognate toxin, AbiEii, is a predicted nucleotidyltransferase (NTase) and member of the DNA polymerase β family. AbiEii specifically bound GTP, and mutations in conserved NTase motifs (I-III) and a newly identified motif (IV), abolished GTP binding and subsequent toxicity. The AbiE systems can provide phage resistance and enable stabilization of mobile genetic elements, such as plasmids. Our study reveals molecular insights into the regulation and function of the widespread bi-functional AbiE Abi-TA systems and the biochemical properties of both toxin and antitoxin proteins.

  5. Sobre el método de la interpretación documental y el uso de las imágenes en la sociología: Karl Mannheim, Aby Warburg y Pierre Bourdieu On the method of documental interpretation and the use of images in Sociology: Kark Mannheim, Aby Warburg and Pierre Bourdieu

    Amalia Barboza Martínez


    Full Text Available El método de interpretación documental elaborado por el sociólogo Karl Mannheim ofrece herramientas de análisis indicadas para integrar materiales visuales en las investigaciones sociológicas. Esto se debe a que Mannheim elaboró este método basándose en los avances metodológicos de los historiadores del arte de su época. En este artículo se presenta en un primer apartado las características centrales de este método. En una segunda parte se muestra que existe dos formas de aplicación de este método a la hora de hacer uso de las imágenes. Por un lado las imágenes pueden ser objeto del método documental. Por otro lado la producción de imágenes pueden servir como técnica para realizar un análisis documental de la sociedad.The method of documental interpretation of the sociologist Karl Mannheim offers appropriate analysing tools to introduce visual data to sociological investigations. Because Mannheim had elaborated his methods based on the methodological progress of art historians. In the first part of this paper the central characteristics of this method will be presented. In the second part the author differences between two ways of application of this method in the way how to use visual data. On the one hand pictures could be objects of analysis of the method of documental interpretation, on the other hand they could also serve to make a documental analysis of the society.

  6. Notes on the Neotropical and Mexican Species of Tetragnatha (Araneae: Tetragnathidae) with Descriptions of Three New Species

    OKUMA, Chiyoko


    Twenty species of the genus Tetragnatha are recognized to occur in the Neotropical and Mexican Regions. Three new species, T. eberhurdi, T. Levii and T. paradoxa, are described. Seventeen species, T. boydi, T, caudata, T. cognata, T. confratemza, T. elongata, T. ethodon, T. gertschi, T. guatemalensis, T. jaculator, T. laboriosa, T. mabelae, T. mexicana, T. nitens, T. pallescens, T. pallida, T. sinuosa and T. tenuissima are redescribed and illustrated. T. jaculator is recorded from the Neotrop...

  7. Revision of the genus Pseudeurybata Hennig (Diptera, Micropezidae, Taeniapterinae).

    Marshall, S A


    The previously monotypic genus Pseudeurybata Hennig is revised to include seven species from Central America and Mexico and one species from South America. Pseudeurybata rufilabris (Enderlein) and Pseudeurybata compeditus (Hennig) are given as new combinations and a lectotype is designated for P. rufilabris (Enderlein). Pseudeurybata browni and P. alces from Costa Rica, P. guatemalensis from Guatemala, P. dasypogon from Mexico, and P. zeta from Colombia and Ecuador are described as new. All species are keyed and illustrated.

  8. Different methods of calculating ankle-brachial index in mid-elderly men and women: the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil).

    Miname, M; Bensenor, I M; Lotufo, P A


    The ankle-brachial index (ABI) is a marker of subclinical atherosclerosis related to health-adverse outcomes. ABI is inexpensive compared to other indexes, such as coronary calcium score and determination of carotid artery intima-media thickness (IMT). Our objective was to identify how the ABI can be applied to primary care. Three different methods of calculating the ABI were compared among 13,921 men and women aged 35 to 74 years who were free of cardiovascular diseases and enrolled in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). The ABI ratio had the same denominator for the three categories created (the highest value for arm systolic blood pressure), and the numerator was based on the four readings for leg systolic blood pressure: the highest (ABI-HIGH), the mean (ABI-MEAN), and the lowest (ABI-LOW). The cut-off for analysis was ABI20% without the inclusion of ABI<1.0 was 4.9%. For ABI-HIGH, ABI-MEAN and ABI-LOW, the increase in percentage points was 0.3, 0.7, and 2.3%, respectively, and the relative increment was 6.1, 14.3, and 46.9%. In conclusion, all methods were acceptable, but ABI-LOW was more suitable for prevention purposes.

  9. 高大冷杉温室Strophosoma象甲的微生物防治%Microbial control of Strophosoma spp. larvae (Coleoptera:Curculionidae) in Abies procera greenery plantations

    夏洛蒂· 尼尔森; Susanne Vestergaard; Susanne Harding; Jφrgen Eilenberg


    In Denmark, the weevils Strophosoma melanogrammum and S.capitatum cause economic damage in Noble fir due to the adult stage feeding on the needles.No chemical treatments of these weevils are allowed in Denmark,so biological control is an attractive solution.We evaluated the potential for microbial control of larvae of Strophosoma spp.based on laboratory bioassays and field applications,taking effect on both target and non-target into consideration,as well as persistence of the applied fungus.In the laboratory Beauveria bassiana,Paecilomyces farinosus and Metarhizium anisopliae were able to infect and cause mycosis in Strophosoma larvae.Among the tested isolates the most virulent isolate was M.anisopliae BIPESCO 5,which resulted in 80 % mortality.In the field experiment M.anisopliae,isolate BIPESCO 5,was applied to the soil as a conidial suspension against larvae of Strophosoma spp.The effect of the fungus on the target population was monitored at a weekly basis by counts of emerging adult weevils during their activity periods.The population of Strophosoma spp.was reduced by up to 60% in treated plots compared to control plots.The non-target effects of M.anisopliae were studied by sampling insects and ticks from both treated and control plots.Seven days after treatment,two sampled insect orders (Hemiptera and Coleoptera) and ticks were found with prevalences of M.anisopliae above 50%,compared to no infection in the insects collected from control plots.Infections in coccinellids were found as long as 277 days after treatment.However,the effect on population level of non-target is still unexplored.The persistence of the fungus was documented by plating a soil suspension onto agar.We documented that conidia of M.anisopliae could persist in the greenery plantation for at least 418 days after application.

  10. Milles näeks riigi abi tööandjale töötervishoiuarst? = Focus of OH physician : what kind of help need employer from the government side? / Tiia Piho

    Piho, Tiia


    Autori arvates peaks riik toetama neid tööandjaid oma töötajate tervise kaitsel elualadel (politseinikud, päästetöötajad, piirivalvurid, meditsiinitöötajad), kus tegevuse eesmärgiks ei ole kasumi tootmine, vaid ühiskonna kaitsmine ja abistamine

  11. A character study on the dead wood of primeval Abies of Sejila Mountain in Southeastern Tibet%西藏色季拉原始冷杉林死亡木特征研究

    罗大庆; 郭泉水; 黄界; 潘刚; 辛学兵; 郑维列


    通过色季拉原始冷杉林死亡木的研究,结果表明:冷杉林死亡木主要由枯立木、拔根倒木、干基折断木和干中折断木4种类型构成,其中干基折断和拔根倒方式形成的倒木是林地死亡木最主要的输入形式;主林层林木的老龄化和心腐病导致了树势的衰弱,极易发生折倒,风害、雪压是诱发立木折倒死亡的直接原因.冷杉林死亡木的现存量为111.53t·hm-2;C、N、P、K、Ca、Mg 6种养分元素的总贮量为60442.69kg·hm-2;原始冷杉林死亡木现存量大、养分贮量高,是目前国内已知的针叶林类型中贮量最高的.

  12. Respuestas fisiológicas y bioquímicas de plántulas de pimiento morrón (Capsicum annuum L) a factores de estrés abiótico.


    En la presente investigación se realizaron dos experimentos con el objetivo de evaluar el efecto de cloruro de sodio (NaCl) y cadmio (Cd) en variables de crecimiento, fisiológicas y bioquímicas de plántulas de pimiento morrón variedad Yolo Wonder. Ambos experimentos fueron conducidos bajo condiciones de invernadero, en cultivo hidropónico en placas flotantes en contenedores de 3 L de capacidad, utilizando la solución nutritiva Steiner al 50%. En el primer experimento se trataron las plántulas...

  13. Estimación del area de las hojas en plantas de trigo bajo diferentes tipos de estrés abiótico Leaf area estimation in wheat plants suffering several kinds of abiotic stress

    D.H. Cogliatti


    Full Text Available En trigo, es posible estimar el área de las hojas (AF utilizando el producto del largo, el ancho de la lámina (LxA y un coeficiente de proporcionalidad (b m. Sin embargo, no hay información sobre la posibilidad de usar el mismo valor del coeficiente para estimar el área en plantas que sufren estrés hídrico, lumínico o nutricional. Para estudiar este punto se realizaron dos experimentos en los cuales se aplicó sequía, sombreo y deficiencias de N y P a plantas de trigo. El coeficiente b m se calculó a partir de la regresión lineal entre AF y LxA y fue similar entre las plantas control y aquellas que sufrieron sequía o deficiencias de N o P, pero fue distinto en plantas sombreadas. El mayor valor de b m en las plantas sombreadas se debió a una mayor proporción del sector medio de la lámina, definido por su forma rectangular. La validación de la posibilidad de usar el b m del control para estimar AF en plantas estresadas se realizó por regresión lineal entre el AF medida y calculada. Se concluye que puede usarse el mismo coeficiente b m para estimar el AF en plantas no estresadas y en plantas que sufren sequía o deficiencias de N o P. El uso del mismo valor del coeficiente b m en plantas sombreadas llevó a una subestimación del AF, la que fue más pronunciada a medida que aumentó el sombreo.In wheat, leaf area (LA can be estimated as the product between length, maximum blade width (LxW and a proportionality coefficient b m. However, it is unknown whether this coefficient is the same in stressed and non stressed plants. In order to study this, two experiments in which drought, shading and N and P deficiencies were applied to wheat plants were performed. The b m coefficient was calculated by linear regression between LA and LxW. The coefficient was similar in control plants as compared to those suffering wilting or N or P-deficiency, but different in shaded plants. The greater b m in shaded plants was due to an increased proportion of the central section of the blade, defined by its rectangular form. Validation of the possibility of using b m of the control to estimate LA in stressed plants was performed by linear regression between measured and calculated LA. It is concluded that the same b m coefficient can be safely used to calculate LA from LxW in non-stressed plants and in those suffering drought or N or P-deficiency. The use of the same coefficient value in shaded plants produced an underestimation of LA, which was more pronounced as shading increases.

  14. Influência de Fatores Abióticos na Infestação de Mosca-Negra-dos-Citros (Aleurocanthus woglumi Ashby em Plantio de Citros em Sistema Agroflorestal no Estado do Pará

    Anderson Silva


    Abstract. An important part of citrus production at Pará state is planted by Agroforestry System (AFS, that presents, amongst major phytosanitary problems, the citrus blackfly, that by severe attacks cause estimated redution of 80% in its’ production. Beside that, it constitutes a quarentenary pest of maximun alert level A2. Given the relevance of this sucking insect and the lack of basic knowledge, as well pest studies associated to agroforestry planting, the objective of this study was to evaluate the abiotic factors influence on blackfly infestation in citrus planting by agroforestry planting at Pará state. This study was carried out at Capitão Poço county, northeast mesoregion of Pará. 12 samplings were made evaluating the presence or abscence of Aleurocanthus woglumi Ashby alive nymphs and/or adults. Correlation analisys was carried out to evaluate abiotic parameters (temperature and precipitation and kriging maps to evaluate Teca plants shading effects on the pest under study infestation. Amongst the main results obtained, there was pest infestation in every evaluated moth; there was temperature influence onto citrus blackfly population regulation and high precipitations reduced the number of plants with A. woglumi presence. Still, it can be inferred that the citrus blackfly infestations present preference for moderate shading intensity. However, changes occuring by the forestry species introduction onto agricultural cultivations must be better investigated.

  15. Non-destructive analysis and detection of internal characteristics of spruce logs through X computerized tomography; Detection et analyse non destructive de caracteristiques internes de billons d'epicea commun (PICEA ABIES (L.) KARST) par tomographie a rayons X

    Longuetaud, F


    Computerized tomography allows a direct access to internal features of scanned logs on the basis of density and moisture content variations. The objective of this work is to assess the feasibility of an automatic detection of internal characteristics with the final aim of conducting scientific analyses. The database is constituted by CT images of 24 spruces obtained with a medical CT scanner. Studied trees are representative of several social status and are coming from four stands located in North-Eastern France, themselves are representative of several age, density and fertility classes. The automatic processing developed are the following. First, pith detection in logs dealing with the problem of knot presence and ring eccentricity. The accuracy of the localisation was less than one mm. Secondly, the detection of the sapwood/heart-wood limit in logs dealing with the problem of knot presence (main source of difficulty). The error on the diameter was 1.8 mm which corresponds to a relative error of 1.3 per cent. Thirdly, the detection of the whorls location and comparison with an optical method. Fourthly the detection of individualized knots. This process allows to count knots and to locate them in a log (longitudinal position and azimuth); however, the validation of the method and extraction of branch diameter and inclination are still to be developed. An application of this work was a variability analysis of the sapwood content in the trunk: at the within-tree level, the sapwood width was found to be constant under the living crown; at the between-tree level, a strong correlation was found with the amount of living branches. A great number of analyses are possible from our work results, among others: architectural analysis with the pith tracking and the apex death occurrence; analysis of radial variations of the heart-wood shape; analysis of the knot distribution in logs. (author)

  16. O lugar da fotografia na construção da obra de Aby Warburg: uma perspectiva cultural para a compreensão da criação imagética

    Paulo César Boni


    Full Text Available Este estudio busca discutir las relaciones planteadas entre la producción fotográfica y la construcción de la teoría warburguiana de posvida de las imá genes ( Nachleben der Antike por medio de su atlas Mnemosyne . Para tanto, utiliza la investigación bibliográca de autores que contribuirán a la elucidación de ese hecho, como Michaud (2013, Didi-Huberman (2013 y Kossoy (1989. Dado el hecho de la creación de paneles fotográcos para la aproximación de las relaciones temporales, Warburg rearma la impor- tancia de la fotografía como un objeto cultural e histórico. Para él, el atlas enfoca en la capacidad de comparación de imágenes en la historia del arte precisamente porque utiliza un panel móvil y práctico para establecer una base antropológica. Se concluye, por tanto, que Warburg usó y se ampa- ró en el potencial de la imagen fotográca para plantear una nueva mirada que rompiera con la perspectiva estática por medio de la imagen en movi- miento y del arte.

  17. 大数据时代外语教师的教育理念和教学能力探析%Exploration of Engl ish Teachers’Educational Concept and Teaching Abi l ity in Big Data Age



    Big Data wi ll change our traditional education by updating the way of knowing the world, weeding out the past short-cuts, and laying more emphasis on imagination.Tremendous progress has been made in the education of foreign languages in China, but some problems sti ll exist l ike learners’ lack of interest, teachers’ lack of advanced teaching concept, and teachers’inabi l ity to integrate IT with foreign language teaching.In Big Data age, we should update our teaching concept from the following four aspects: developing a diversified and personal ized foreign languages teaching concept by making use of Big Data; establ ishing a humanistic and dynamic teaching mechanism through authorized and patterned method; implementing empirical and scientific class teaaching by abandoning the old teaching way; practicing aptitude-based teaching by correcting the previous averaged teaching.Furthermore, we need to improve the three ski lls of foreign language teaching: making full use of fl ipping class to integrate traditional teaching with onl ine teaching;concluding traditional foreign languages teaching methods to form a teaching strategy suitable for Big Data age; attaching importance to learning, accumulation and classification to provide more advice, resources, and evaluation methods for students.%大数据将从认识世界的新方式、淘汰过去的捷径和想象力将比知识更重要等三个方面改变教育。我国外语教育已取得了长足发展和进步,但也明显存在学生学习兴趣不足、教师教学理念不够先进及整合教育技术与外语教学意识和能力薄弱等问题。面临大数据技术和大数据时代,我们的外语教育首先要进行四个教育理念的更新,即利用大数据去标准化、统一化模式,形成多样化和个性化的外语教育观念;利用大数据去权威化、固态化套路,建立人文化和动态化的外语教育机制;利用大数据告别简单经验式、思辨式、自上而下的教学,实施实证式、科学式、自下而上的课堂教学;利用大数据抛弃传统平均化教育,实施差异化的教育,真正因材施教。然后是实现三个教学能力的提高,即充分利用慕课和翻转课堂,实行线上和线下相融合的外语教育方式;整合传统课堂的外语教学法,打造大数据时代的外语教学策略;加强学习、注重积累、学会分类,为学生提供更多的学习资源、学习建议和评价方式。

  18. DNA data

    National Oceanic and Atmospheric Administration, Department of Commerce — Raw DNA chromatogram data produced by the ABI 373, 377, 3130 and 3730 automated sequencing machines in ABI format. These are from fish (primarily Sebastes spp.,...

  19. Acquired brain injury services in the Republic of Ireland: experiences and perceptions of families and professionals.

    McDermott, Garret L


    This study aimed to highlight the experiences and perceptions of rehabilitation services among families of people with Acquired Brain Injury (ABI) and among professionals working in ABI rehabilitation services in Ireland.

  20. FGA Variants

    ... Smirnova 124 Armed Forces Central Forensic ... ABI 310 PS Michael Mathews, Illinois State Police Observed in five forensic samples and in each ... M U F S C 46.1 329.37 ABI 310 PS Ryan Porter, ...

  1. Experiences from Cochlear Implantation and Auditory Brainstem Implantation in Adults and Children : Electrophysiological Measurements, Hearing Outcomes and Patient Satisfaction

    Lundin, Karin


    Cochlear implants (CIs) and auditory brainstem implants (ABIs) are prostheses for hearing used in patients with profound hearing impairment. A CI requires an operational cochlear nerve to function in contrast to an ABI. ABIs were initially designed for adult patients with neurofibromatosis type 2 (NF2), suffering from bilateral vestibular schwannomas. Now ABIs are also used for patients, both adults and children, with congenital cochlear malformations, cochlear nerve hypoplasia/aplasia, and c...

  2. NCBI nr-aa BLAST: CBRC-DMEL-08-0064 [SEVENS

    Full Text Available b|AAB59125.1| 13.8 kd ORF [Plasmid ColE1] gb|ABI93156.1| hypothetical protein [Cloning vector pOri1] gb|ABI9...3161.1| hypothetical protein [Cloning vector pCW] gb|ABI93166.1| hypothetical protein [Cloning vector pCW1]|ABI93171.1| hypothetical protein [Cloning vector pCW2] NP_040360.1 9e-63 95% ...

  3. Eesti transpordi infrastruktuuri ootavad suured toetused / Tanel Tang

    Tang, Tanel


    EL-iga liitumisel avanevad Eestile Euroopa Regionaalarengu fond (ERDF) ja Ühtekuuluvusfond, mis tähendab transpordisektorile miljardite kroonide suurust abi maanteede, raudteede, lennujaamade ning sadamate ehitamiseks ja rekonstrueerimiseks. Vt. samas: Eestile tulnud abi PHARE ja ISPA fondist ; Ühtekuuluvusfond ja Euroopa regionaalarengu fond (ERDF) toetavad Eestit miljonite kroonidega. Diagramm: EL-i abi Eesti transpordisektorile

  4. Identification and bioinformatics comparison of two novel phosphatases in monoecious and gynoecious cucumber lines

    Pawełkowicz, Magdalena E.; Wojcieszek, Michał; Osipowski, Paweł; Krzywkowski, Tomasz; PlÄ der, Wojciech; Przybecki, Zbigniew


    Two Arabidopsis thaliana genes from the PP2C family of protein phosphatases (AtABI1 and AtABI2) were used to find orthologous genes in the Cucumis sativus L. cv. Borszczagowski (cucumber) genome. Cucumber has been used as a model plant for sex expression studies because although it has been defined as a monoecious species, numerous genotypes are known to produce only female, only male, or hermaphroditic flowers. We identified two new orthologous genes of AtABI1 and AtABI2 in the cucumber genome and named them CsABI1 and CsABI2. To determine the relationships between the regulation of CsABI1 and CsABI2 and flower morphogenesis in cucumber, we performed various computational analyses to define the structure of the genes, and to predict regulatory elements and protein motifs in their sequences. We also performed an expression analysis to identify differences in the expression levels of CsABI1 and CsABI2 in vegetative and generative tissues (leaf, shoot apex, and flower buds) of monoecious (B10) and gynoecious (2gg) cucumber lines. We found that the expressions of CsABI1 and CsABI2 differed in male and female floral buds, and correlated these findings with the abscisic acid signaling pathways in male and female flowers.

  5. 78 FR 30399 - United States v. Anheuser-Busch InBev SA/NV, Grupo Modelo S.A.B de C.V.; Proposed Final Judgment...


    ... lower its prices in local Texas markets, and adjust its marketing strategy to better respond to... do. 4. In contrast, Modelo has resisted ABI-led price hikes. Modelo's pricing strategy--``The... pressure'' on ABI by pursuing a competitive strategy directly at odds with ABI's well-established...

  6. Fuel wood properties of some oak tree species of Manipur, India.

    Meetei, Shougrakpam Bijen; Singh, E J; Das, Ashesh Kumar


    Five indigenous oak tree species, i.e., Castanopsis indica (Roxb. ex Lindl.) A.DC., Lithocarpus fenestratus (Roxb.) Rehder, Lithocarpus pachyphyllus (Kurz) Rehder, Lithocarpus polystachyus (Wall. ex A.DC.) Rehder and Quercus serrata Murray were estimated for their wood properties such as calorific value, density, moisture content and ash content from a sub-tropical forest of Haraothel hill, Senapati District, Manipur. Wood biomass components were found to have higher calorific value (kJ g(-)) than bark components. The calorific values for tree species were found highest in L. pachyphyllus (17.99 kJ g(-1)) followed by C. indica (17.98 kJ g1), L. fenestratus (17.96 kJ g"), L. polystachyus (17.80 kJ g(-1)) and Q. serrata (17.49 kJ g(-1)). Calorific values for bole bark, bole wood and branch bark were found significantly different (F > 3.48 at p = 0.05) in five oak tree species. Percentage of ash on dry weight basis was found to be highest in Q. serrata (4.73%) and lowest in C. indica (2.19%). Ash content of tree components gives a singnificant factor in determining fuelwood value index (FVI). Of all the five oak tree species, Q. serrata exhibited highest value of wood density (0.78 g cm-) and lowest was observed in C. indica (0.63 g cm(-3)). There was significant correlation between wood density (p L. pachyphyllus (898.41)> L. polystachyus (879.02)> L. fenestratus (824.61)> Q. serrata (792.50). Thus, the present study suggests that C. indica may be considered as a fuelwood oak tree species in Manipur.

  7. Regulation of the abscisic acid response by protein phosphatase 2C-interacting proteins ABP7 and ABP9 in Arabidopsis thaliana

    Ma-Lauer, Yue


    The protein phosphatases 2C ABI1 and ABI2 are negative regulators in signal transduction of the phytohormone abscisic acid (ABA). The aim of this work is to characterize two homologous proteins ABP7 and ABP9, which were identified as interacting partners of ABI2 in the yeast two-hybrid system. In protoplasts, ABP7 and ABP9 interacted with both ABI1 and ABI2 in the nucleus and the cytosol. Overexpression of ABP7 and ABP9 resulted in dramatic inductions of ABA-induced gene expression in div...

  8. Peripheral artery disease (PAD) screening in the asymptomatic population

    Sillesen, Henrik; Falk, Erling


    and feet (lower limb), which in the Western world is caused by atherosclerosis if not previous trauma. Whereas severity of intermittent claudication is only poorly related to ABI, cardiovascular outcomes are as follows: the lower the ABI the higher the incidence of cardiovascular events and death....... Measuring ABI identifies asymptomatic persons at increased risk of cardiovascular morbidity and mortality: an ABI 25% in people between 80 and 90 years of age. The majority of persons with reduced ABI are asymptomatic and therefore unaware of the increased risk they are living with, thus, screening...

  9. Comportamiento y rendimiento en aserrío de trozas de Terminalia amazonia de 6 años de edad proveniente de la zona sur de Costa Rica


    Las primeras experencias llevadasa cabo en CostaRica con la reforestación con especies nativas se dieron en la Estación Biológica La Selva por parte de la Organización de los Estudios Tropicales (OET) a partir del año de 1985 en la zona de Sarapiquí, provincia de Heredia. Por parte de la mencionada organización se han establecido ensayos con especies tales como Dipteryx panamensis, Vochysia ferruginea, Vochysia guatemalensis, entre otros grupos de especies de gran interés para el país.


    Hermes VEGA


    Full Text Available Se registran 11 novedades para la flora de Honduras a partir de ejemplares recolectados en el Parque Nacional Montaña Celaque. Los nuevos registros son: Tillandsia mateoensis (Bromeliaceae, Diastema affine (Gesneriaceae, Scutellaria costaricana (Lamiaceae, Miconia contrerasii (Melastomataceae, Acianthera violacea, Dendrophylax porrectus, Epidendrum santaclarense, Lepanthes enca-barcenae, L. fratercula, L. isabelae y Sarcoglottis schaffneri (Orchidaceae. Por otra parte, Potosia guatemalensis es sinonimizado bajo S. schaffneri. Se incluyen fotos de todas las especies, hábitats y un mapa de distribución con sus localidades en Honduras.

  11. Nuevos registros para la flora de Honduras y el Parque Nacional Montaña de Celaque.

    Hermes Vega


    Full Text Available Se registran 11 novedades para la flora de Honduras a partir de ejemplares recolectados en el Parque Nacional Montaña Celaque. Los nuevos registros son: Tillandsia mateoensis (Bromeliaceae, Diastema affine (Gesneriaceae, Scutellaria costaricana (Lamiaceae, Miconia contrerasii (Melastomataceae, Acianthera violacea, Dendrophylax porrectus, Epidendrum santaclarense, Lepanthes enca-barcenae, L. fratercula, L. isabelae y Sarcoglottis schaffneri (Orchidaceae. Por otra parte, Potosia guatemalensis es sinonimizado bajo S. schaffneri. Se incluyen fotos de todas las especies, hábitats y un mapa de distribución con sus localidades en Honduras.

  12. Lesiones causadas por helmintos del aparato digestivo en peces estuarinos de la Laguna Tres Palos, Guerrero, México

    Ramón Vázquez Núñez; José Ramírez Lezama; David Osorio Sarabia; Larisa Adriana Chávez Soriano; Fernando Constantino Casas


    Se realizó el presente estudio con el fi n de identificar y comparar las lesiones macro y microscópicas asociadas a helmintos en el tracto digestivo del Ariopsis guatemalensis (“cuatete”) y Eleotris picta (“alahuate”) de la Laguna de Tres Palos, Guerrero, México. En Mayo, Julio y Diciembre del 2001 se colectaron 30 peces de cada especie. A 15 individuos de cada muestra se les practicó el examen histopatológico y a los 15 restantes el examen parasitológico. Se identifi caron tres grupos de hel...

  13. AcEST: BP915822 [AcEST

    Full Text Available r_hit_id Q6Y9M7 Definition tr|Q6Y9M7|Q6Y9M7_9FILI Ribulose bisphosphate carboxylase large chain (Fragment) OS=Anogramma...9 6e-78 >tr|Q6Y9M7|Q6Y9M7_9FILI Ribulose bisphosphate carboxylase large chain (Fragment) OS=Anogramma leptop...FILI Ribulose bisphosphate carboxylase large chain (Fragment) OS=Anogramma guatemalensis GN=rbcL PE=3 SV=1 L

  14. Evaluation of a multicapillary electrophoresis instrument for mitochondrial DNA typing.

    Stewart, John E B; Aagaard, Patricia J; Pokorak, Eric G; Polanskey, Deborah; Budowle, Bruce


    Laser-induced detection of fluorescent labeled PCR products and multi-wavelength detection (i.e., multicolor analysis) enables rapid generation of mtDNA sequencing profiles. Traditionally, polyacrylamide slab gels have been used as the electrophoretic medium for mtDNA sequencing in forensic analyses. Replacement of slab gel electrophoresis with capillary electrophoresis (CE) can facilitate automation of the analytical process. Automation and high throughput can be further enhanced by using multicapillary electrophoretic systems. The use of the ABI Prism 3100 Genetic Analyzer (ABI 3100, Applied Biosystems, Foster City, CA) as well as the ABI Prism 310 Genetic Analyzer (ABI 310, Applied Biosystems, Foster City, CA) were evaluated for mtDNA sequencing capabilities and compared with sequencing results obtained on the platform currently in use in the FBI Laboratory (the ABI Prism 377 DNA Sequencer, ABI 377, Applied Biosystems, Foster City, CA). Various studies were performed to assess the utility of the ABI 3100, as well as the ABI 310 for mtDNA sequencing. The tests included: comparisons of results obtained among the ABI 3100, the ABI 310 and the ABI 377 instruments; comparisons of results obtained within and between capillary arrays; evaluation of capillary length; evaluation of sample injection time; evaluation of the resolution of mixtures/heteroplasmic samples; and evaluation of the sensitivity of detection of a minor component with reduced template on the ABI 3100. In addition, other studies were performed to improve sample preparation; these included: comparison of template suppression reagent (TSR, Applied Biosystems, Foster City, CA) versus formamide; the use of Performa DTR Gel Filtration Cartridges (Edge BioSystems Inc., Gaithersburg, MD) versus Centri-Sep Spin Columns (Princeton Separations, Adelphia, NJ) for product purification after cycle sequencing; and sample stability after denaturation. The data support that valid and reliable results can be obtained

  15. Generating code adapted for interlinking legacy scalar code and extended vector code

    Gschwind, Michael K


    Mechanisms for intermixing code are provided. Source code is received for compilation using an extended Application Binary Interface (ABI) that extends a legacy ABI and uses a different register configuration than the legacy ABI. First compiled code is generated based on the source code, the first compiled code comprising code for accommodating the difference in register configurations used by the extended ABI and the legacy ABI. The first compiled code and second compiled code are intermixed to generate intermixed code, the second compiled code being compiled code that uses the legacy ABI. The intermixed code comprises at least one call instruction that is one of a call from the first compiled code to the second compiled code or a call from the second compiled code to the first compiled code. The code for accommodating the difference in register configurations is associated with the at least one call instruction.

  16. Hearing Restoration with Auditory Brainstem Implant

    NAKATOMI, Hirofumi; MIYAWAKI, Satoru; KIN, Taichi; SAITO, Nobuhito


    Auditory brainstem implant (ABI) technology attempts to restore hearing in deaf patients caused by bilateral cochlear nerve injury through the direct stimulation of the brainstem, but many aspects of the related mechanisms remain unknown. The unresolved issues can be grouped into three topics: which patients are the best candidates; which type of electrode should be used; and how to improve restored hearing. We evaluated our experience with 11 cases of ABI placement. We found that if at least seven of eleven electrodes of the MED-EL ABI are effectively placed in a patient with no deformation of the fourth ventricle, open set sentence recognition of approximately 20% and closed set word recognition of approximately 65% can be achieved only with the ABI. Appropriate selection of patients for ABI placement can lead to good outcomes. Further investigation is required regarding patient selection criteria and methods of surgery for effective ABI placement. PMID:27464470

  17. Virtual environments as a tool for people with acquired brain injury

    Wallergård, Mattias


    People with acquired brain injury (ABI) often have problems leading an independent life due to impaired cognitive abilities. One way to address this is to let the patients practise activities of daily living as part of their rehabilitation process. However, some everyday activities can be difficult, inconvenient or risky to practise. The demands of the environment can also have an impact on the independence of an individual with ABI. Today, the involvement of people with ABI in the design of ...

  18. Каузальные конструкции со словом абие в текстах житий(Causal Constructions with the Word абие in the Hagiographic Texts

    Tamara Lönngren


    Full Text Available The aim of this article is to characterize and classify the causal constructions in which the word abie occurs in a collection of Hagiographic Texts from the fifteenth century (the author of the collection is the Russian monk Nil Sorsky, and to investigate the realization of one of the meanings of abie in a certain type of causal construction. The concluding part presents a semantic description of the word abie, which is frequently used in these constructions.

  19. Assistive technologies for brain-injured gamers

    Colman, Jason; Gnanayutham, Paul


    This chapter surveys assistive technologies which make video games more accessible for people who have an acquired brain injury (ABI). As medical care improves, an increasing number of people survive ABI. Video games have been shown to provide therapeutic benefits in many medical contexts, and rehabilitation for ABI survivors has been shown to be facilitated by playing some types of video game. Therefore, technologies which improve the accessibility of games have the potential to bring a form...

  20. Synthesis and Luminescent Chemosensory Behavior of N,N'-bis(p-aminophenyl)-1,5-bis(p-(tetraethyleneglycoloxy)phenyl)anthracene-2,3,6,7-tetracarboxyl bisimide

    Ilhan, Faysal; Tyson, Daniel S.; Meador, Michael A.


    We have utilized the photoenolization reaction of a dibenzoyl-p-xylene derivative to synthesize two new, highly substituted anthracene-based molecules. ABI-NO2 and ABI-NH2 maintained photostability with longer wavelength absorption and emission spectra as compared to unsubstituted anthracene. Conversion of the periphery nitro groups to amines created an internal photoinduced electron transfer scenario that quenched the luminescence of ABI-NH2. Reversible or irreversible reaction of the attached amines resulted in fluorescence recovery. Sensitivity, of ABI-NH2 and other potential derivatives, to toxic chemicals may prove useful for early detection systems.

  1. Isiku paigutamine kinnisesse asutusse psühhiaatrilise ravi eesmärgil : [magistritöö] / Mai-Liis Meigo ; Tartu Ülikool, õigusteaduskond ; juhendajad: Kai Härmand, Kristjan Kask

    Meigo, Mai-Liis


    Isiku tahtevastasest paigutamisest raviasutusse (tsiviilkohtumenetluse seadustik, psühhiaatrilise abi seadus), isiku põhiõigustest ja sellest tulenevast kohtupraktikast tahtevastase ravi läbiviimisel


    Full Text Available RYREPEATBNNAPA S000264 16-Feb-2001 (last modified) seki RY repeat found in RY/G box... (the complex containing the two RY repeats and the G-box) of napA gene in Brassica napus (B.n.); Found betw...een -78 and -50; Required for seed specific expression; See S000262, S000263; dist B ABRE mediated transacti...vation by ABI3 adn ABI3-dependent response to ABA; a tetramer of the composite RY.../G complex mediated only ABA-independent transactivation by ABI3; B2 domain of ABI3 is necessary for ABA-independent and ABA-depe

  3. Crescimento micelial in vitro de cinco linhagens de Agaricus bisporus submetidas a diferentes condições de temperatura = In vitro mycelium growth of five Agaricus bisporus strains submitted to different temperature conditions

    Meire Cristina Nogueira de Andrade


    Full Text Available Avaliou-se o crescimento micelial in vitro das linhagens ABI-05/03, ABI-06/04, ABI-04/02, ABI-06/05 e ABI-01/01 de Agaricus bisporus em meios de cultura sólidos à base de composto. As avaliações foram realizadas por meio de medições de quatro diâmetros das colônias, a cada 48h, durante 12 dias de incubação, no escuro, a 20 e 25ºC. O delineamento experimental foi o de blocos casualizados, com uso do teste de Tukey para a comparação das médias. Com base nos resultados obtidos, verificou-se que: o crescimento micelial de A. bisporus é influenciado pela temperatura de incubação; a temperatura de 25°C foi mais favorável para o crescimento micelial de todas as linhagens de A. bisporus; na temperatura de 20°C, o melhor crescimento foi obtido com as linhagens ABI-06/05 e ABI-01/01; na temperatura de 25°C, a linhagem ABI-01/01 apresentou crescimento significativamente maior que todas as demais.The in vitro mycelium growth of Agaricus bisporus strains ABI-05/03, ABI-06/04, ABI-04/02, ABI-06/05 and ABI-01/01 was evaluated in solidculture media made up of compost. Evaluations were performed by means of measurements of four diameters of the colonies, every 48 hours, during 12 days of incubation in darkness under 20 and 25ºC. The experimental design consisted of randomized blocks, using the Tukey test to compare averages. Based on the obtained results, it was verified that: mycelium growth of A. bisporus is influenced by the temperature of incubation; the temperature of 25°C was more favorable to the mycelium growth of all A. bisporus strains; under the temperature of 20°C, the best growth was obtained with strainsABI-06/05 and ABI-01/01 and, under the temperature of 25°C, strain ABI-01/01 showed significantly higher growth than all other strains.

  4. The risk factors for abnormal ankle-brachial index in type 2 diabetic patients and clinical predictive value for diabetic foot



    Objective To investigate the prevalence of diabetic foot (DF) and the normal,high and low ankle brachial index (ABI) in type 2 diabetic patients and explore the risk factor for abnormal ABI and the clinical predictive value for DF.Methods A total of 2 681 type 2 diabetic patients who visited our hospital between January,2007and December,2009 were enrolled in the study.The clinical data were analyzed and the risk factors for abnormal ABI were determined by logistic regression analysis.Results ABI was normal (0.9-<1.3) in 2 362 cases

  5. Parts toetab eksporti miljardigarantiiga / Juhan Parts ; intervjueerinud Gert D. Hankewitz, Andrus Karnau

    Parts, Juhan, 1966-


    Intervjuu majandus- ja kommunikatsiooniminister Juhan Partsiga, kes vastab küsimustele ekspordiettevõtetele riiklike garantiide andmise teemal. Vt. samas: Ekspordigarantiid; Padar pakub abi laenuvõtjatele

  6. 15 CFR 30.51 - Statistical information required for import entries.


    ... entry summary documentation (CBP-7501). Refer to the Customs and Trade Automated Interface Requirements for instructions on submitting an ABI electronic record, or instructions for completing CBP-226...

  7. Factores abióticos que influencian la germinación de seis especies herbáceas de la zona árida de Chile Abiotic factors effects influencing the germination of six herbaceous species of Chilean arid zone

    Paola A Jara


    Full Text Available La zona árida del norte de Chile presenta un clima seco que impide la emergencia masiva de especies vegetales, excepto cuando se producen escasas e irregulares precipitaciones que modifican las características de aridez y estimulan la germinación de semillas. Los principales factores externos que modifican estructuras y condiciones internas de las semillas son el tiempo de hidratación, iluminación, temperatura y escarificación. En este trabajo se abordaron dos interrogantes: (a ¿la germinación de semillas de seis especies de zonas áridas es regulada por diferentes factores externos o presentan respuestas comunes a una combinación de estímulos? y (b ¿existe correspondencia entre las condiciones germinativas establecidas en laboratorio y las determinadas in situ? Para resolver estas interrogantes se sometieron seis especies herbáceas endémicas y nativas del norte de Chile (Cistanthe salsoloides, Leucocoryne purpurea, Pasithea coerulea, Placea amoena, Schizanthus litoralis y Trichopetalum plumosum a dos experimentos germinativos con combinaciones factoriales de tiempo de hidratación, temperatura e iluminación. Solo S. litoralis fue sometido a un experimento adicional de escarificación y deshidratación. Los resultados determinaron que todas las especies en estudio presentaron respuestas germinativas comunes frente a determinados factores externos, con una máxima germinación al sobrepasar un umbral de 96 h de hidratación y permanecer a niveles de temperatura de 10 a 25 ºC. La respuesta frente a iluminación fue especie dependiente. Schizanthus litoralis solo responde a estímulos externos previo a ser escarificada su semilla. Además, se determinó que existe concordancia entre los factores germinativos determinados en laboratorio y las condiciones naturales de germinación. Por lo tanto, las semillas de las especies en estudio presentan umbrales germinativos semejantes, y las técnicas de germinación en laboratorio deben ser determinadas por las características ambientales del lugar de procedencia y de la época de germinación naturalThe arid zone of northern Chile has a dry climate that prevents the massive emergency of plant species. The exception to this general trend arises when scarce and irregular rainfall events occur, modify the environmental humidity, and stimulate the germination of seeds. The main external factors that modify the internal nature of the seeds are the hydration time, light, temperature and scarification. In this work two questions were addressed: (a is the germination of seeds of arid zones regulated by independent external factors or by a combined array of stimuli? and (b do exist correspondence between laboratory and in situ germinative conditions?. Seeds of six native and endemic herbaceous species of the north of Chile (Cistanthe salsoloides, Leucocoryne purpurea, Pasithea coerulea, Placea amoena, Schizanthus litoralis y Trichopetalum plumosum were subjected to two germination experiments, with factorial combinations of hydration time, temperature, light, dehydration and scarification. Schizanthus litoralis, was subjected to an aditional scarification-dehydration experiment (experiment 3. Results showed a common response of all the species in study to certain external factors. Maximum germination percentages were reached when exceeding a threshold of 96 h of hydration and at temperatures of 10 to 25 ºC. Light response was species-dependent. Agreement was found between the germinative conditions determined in laboratory and natural conditions of germination. Therefore, the seeds of plant species of arid zones display similar germinative thresholds and the techniques of germination in laboratory must be determined by the environmental characteristics of the place of origin and the time of natural germination

  8. The EvaIuation of Auditory and VerbaI AbiIity in 107 Pre-LinguaIIy Deaf ChiIdren with CochIear ImpIantation%语前聋儿童人工耳蜗植入后听觉言语能力评估

    郭思荃; 唐冬梅; 李华伟; 陈兵; 戴春富


    目的:评估语前聋儿童人工耳蜗植入术后听觉言语能力的发展规律及其影响因素。方法对107例语前聋儿童人工耳蜗植入术后,应用婴幼儿有意义听觉整合量表(infant-toddler meaningful auditory integration scale/meaningful auditory integration scale ,IT -MAIS/MAIS)、听觉行为分级(categories of auditory performance , CAP)和言语可懂度分级(speech intelligibility rating ,SIR)三种问卷评估其听觉言发展水平,分析植入时间、植入年龄、实际年龄、术前助听器使用情况等对其的影响。结果107例语前聋儿童的植入年龄、植入时间对IT -M AIS/MAIS得分率有显著影响,植入时间、实际年龄对CAP、SIR分级有显著影响(P值均<0.05),而术前是否佩戴助听器对IT -MAIS/MAIS得分率、CAP、SIR分级均无显著影响(均为 P>0.05)。结论植入年龄、植入时间、实际年龄对语前聋儿童人工耳蜗植入后的听觉言语能力发展有显著影响,而术前是否佩戴助听器对其听觉言语能力的影响有待后续研究证实。%Objective To evaluate the auditory and verbal abilities of cochlear implantees using question_naires ,to explore basic rules of the development of auditory and verbal abilities ,and to analyze their relationships with some influencing factors .Methods A retrospective analysis of 107 cases of pre-lingually deaf children with cochlear implantation was conducted with Infant-Toddler Meaningful Auditory Integration Scale/Meaningful Audi_tory Integration Scale (IT -MAIS/MAIS) ,categories of auditory performance (CAP) and speech intelligibility rat_ing (SIR) for the evaluation of auditory and verbal abilities .An analysis was performed to assess influencing fac_tors ,such as postoperative time ,age at implantation ,physical age at follow -up ,time with hearing aids before im_plantation .SAS 9 .1 .3 was used in statistical analysis .ResuIts A total of 107 pre-lingually deaf children (48 males and 59 females) participated in this study .The postoperative time ranged from 0 to 49 months ,with an average of 9 .0 ± 10 .9 months .The results showed the postoperative time and age at implantation had significant influences on the scores of IT -MAIS/MAIS ,and postoperative time and physical age at follow -up had significant influences on the scores of CAP/SIR (P0 .05) .ConcIusion The ages at implantation ,postoperative time and physical age had significant influences on the auditory and verbal abilities of pre-lingually deaf children with cochlear implanta_tion .Further studies are needed to explore their relationships with hearing aids usage before implantation .

  9. Planktonic crustacean assemblages of three reservoirs from the Mexican Central Plateau: relationships with biotic and abiotic factors Crustáceos planctónicos de tres embalses de la Mesa Central Mexicana: relaciones con factores bióticos y abióticos

    Gloria Ana María Arroyo Bustos


    Full Text Available The reservoirs Trinidad Fabela (TF, Ignacio Ramírez (IR and Tepuxtepec (T are located along an altitude gradient in the upper Lerma Basin of the Mexican Central Plateau. Between July 1993 and June 1994, the planktonic crustacean assemblages of these systems were dominated by seven cladoceran species and five copepod species. Specific richness ranged from 13 species in TF to 16 species in T, out of a total 20 species. Nine species were common to all three systems, while the rest were absent from at least one reservoir. Canonic correspondence analysis showed that the structure and seasonal variation of these assemblages are regulated by factors directly related (transparency and dissolved oxygen or inversely related (turbidity, temperature, mineralization and eutrophication to the altitude at which the reservoirs are located. TF had the lowest levels of mineralization and the lowest specific richness, as well as the highest plankton diversity and mean density, while T had the highest mineralization and highest specific richness, but showed the lowest density. Crustacean mean size was smaller in T than in TF, possibly due to predation by fish in the former system and the presence of fish predators (waterfowl in the latter, as well as the impact of the different hydrological regime and release schedules of the reservoirs.Se estudiaron los embalses Trinidad Fabela (TF, Ignacio Ramírez (IR y Tepuxtepec (T que se localizan en un gradiente de altitud en la subcuenca del Alto Lerma en la Mesa Central de México. La asociación de crustáceos planctónicos estuvo dominada por siete especies de cladóceros y cinco copépodos y la riqueza específica varió entre 13 y 16 especies con un total de 20 especies; nueve de éstas resultaron comunes a todos los embalses y las otras no se encontraron en al menos uno de ellos. El análisis de correspondencias canónicas mostró que la estructura y la variación estacional de las asociaciones de crustáceos planctónicos están regulados por factores que están directamente (transparencia y oxígeno disuelto o inversamente (turbiedad, temperatura, grado de mineralización y de eutrofización relacionados a la altitud de los tres embalses. TF fue el embalse con el menor grado de mineralización y la menor riqueza específica y con la mayor diversidad y densidad de plancton. T tuvo la mayor mineralización y riqueza específica, pero la menor densidad. Los crustáceos de T presentaron tallas medias menores que los de TF, posiblemente como un efecto de depredación de la ictiofauna en T y de la presencia de aves acuáticas piscívoras en TF, además del diferente régimen hidrológico de los embalses.

  10. Abiotic gradients drive floristic composition and structure of plant communities in the Monte Desert Gradientes abióticos dirigen la composición florística y la estructura de las comunidades de plantas en el Desierto del Monte



    Full Text Available Defining plant communities in desert zones is difficult due to large scale homogeneity and small scale heterogeneity, thus making provision of systematic information for conservation decisions problematic. We analysed plant communities of the most arid sector of Monte Desert for structure, plant composition and environmental variables. Small-scale variables such as slope, rock cover, bare ground and litter, as well as large-scale ones such as species diversity, composition and similarity within and between sites were included. Analyses of floristic composition showed the difficulty of segregating distinct communities due to high internal heterogeneity and overlap between the different sites. Only mesquite woodlands, a community situated at the extreme of the soil moisture-gradient was segregated. Ordination on structural variables was somewhat more successful in segregating communities on the basis of substrate type and of tree and shrub cover. Our results showed the difficulty distinguishing plant communities in temperate deserts, suggesting the existence of relatively stable assemblages of species at the extremes of the gradients and of great heterogeneity within and between sites. They cannot be defined by floristic variables solely, but require environmental information also.La definición de comunidades discretas de plantas en zonas desérticas es complejo debido tanto a su homogeneidad a gran escala como a su heterogeneidad a pequeña escala, lo que acaba generando dificultades para la toma de decisiones de conservación. En este trabajo analizamos las comunidades de plantas del sector más árido del Desierto del Monte en función de su estructura y composición florística. Se han utilizado también variables ambientales estimadas a pequeña escala como la pendiente o la superficie de roca, suelo desnudo y hojarasca, así como variables que operan a mayor escala como la diversidad de especies, la composición florística y la similitud dentro y entre localidades. Los análisis de composición florística resaltaron la dificultad para segregar diferentes comunidades debido al elevado nivel de heterogeneidad interna y de solapamiento florístico entre las distintas localidades estudiadas. Solo la comunidad situada en el extremo del gradiente de humedad edáfica, el algarrobal, se segregó del resto. La ordenación realizada sobre las variables estructurales en función del tipo de sustrato y de la cobertura arbórea y de matorral segregó mejor las diferentes comunidades. Nuestros resultados muestran la dificultad para diferenciar comunidades de plantas en desiertos templados, sugiriendo la existencia de ensamblajes de especies relativamente estables en los extremos de los gradientes y de una gran heterogeneidad dentro y entre localidades. Las comunidades de plantas, por tanto, no pueden ser definidas únicamente por variables florísticas, sino que es preciso incluir información ambiental.

  11. Conciliation of Hassan bin Ali bin Abi Talib and the end of strife صُّلْحُ الحسن بن علي بن أبي طالب ونهاية الفتنة

    Mohamed Derradj


    يعتبر صلح الحسن بن علي بن أبي طالب "رضي الله عنه"، وتنازله عن الخلافة لمعاوية بن أبي سفيان منعطفًا تاريخيًا هامًا انتقلت به رئاسة الدولة الإسلامية من طور الخلافة الراشدة إلى طور الملكية السياسية. ولئن كان الكثير من الباحثين يرى في أحداث الفتنة الكبرى- وما نتج عنها من آثار سياسية بالغة التأثير على مستقبل الإسلام السياسي- انعطافًا كبيرًا عن خط الإسلام الأصيل، إلا أن ثمة مَن يرى أنها ليست كذلك، بل كانت تعبيرًا عنيفًا عن تحولات عميقة جدت في تكوين بنية المجتمع الإسلامي الذي بدأ ينتقل إلى عهد جديد ...

  12. Physiological and biochemical contributions to the problems of smoke damage research. III. The influence of strong spasmodic doses of SO/sub 2/ on the CO/sub 2/ absorption and on certain constituents of the needles of Picea abies and Pinus mugo under laboratory conditions

    Vogl, M.; Boertitz, S.; Polster, H.


    Potted young plants show assimilation depressions of various degrees before smoke damage becomes visible. Needles or parts of needles without any outward injuries can recover within a few weeks. Individual differences in outward appearance as well as in physiological behavior were found with the species investigated. Contrary to assimilation, the variations of pH-values as well as those of the sugar-, and amino-acid balances of saps pressed from needles are measurable within the lethal region only. 18 references, 9 figures.

  13. 腐乳中高效氨氮降解菌的筛选、鉴定与降解特性研究%IsoIation and Identification of High Effective Ammonia Nitrogen Degradation Bacterium in Fermented Bean Curd and Research on Its Degradation AbiIity

    冯爱娟; 叶茂; 顾宗珠; 邓毛程


    以500 mg/L的高浓度(NH4)2 SO4为唯一氮源的选择培养基,从市售的腐乳中分离筛选出1株对高氨氮含量中氨氮降解效率较高的菌株 FR08,经形态学和生理特性初步鉴定其为巨大芽孢杆菌。实验结果表明:在初始氨氮质量浓度为500 mg/L,初始pH 值为7.0,培养温度为28℃时,该菌株48 h对氨氮降解率为73.64%。该菌可应用于养殖废水、生活污水及氨氮污染较严重的土壤的氨氮处理。%A high effective bacterium FR08 degrading ammonia nitrogen has been isolated from the fermented bean curd by the selected culture medium with (NH4 )2 SO4 as exclusive nitrogen source. The degrading rate of NH4 +-N by FR08 is over 73.64% with 48 h at initial concentration NH4 +-N of 500 mg/L,initial pH of 7.0,culture temperature of 28 ℃.FR08 strain is identified as Bacillus aryabhattai primarily.It can be treated to control the serious problems of ammonia nitrogen pollution in sewage,urban sewage and soil.

  14. Screening and Identification of a Strain of Lactobacillus with ChoIesteroI-reducing AbiIity and Its AppIication in PickIes%一株降胆固醇乳酸菌的筛选、鉴定及在发酵泡菜中的应用

    朱奇奇; 蒲博; 王周; 张驰翔; 焦士蓉


    对降胆固醇乳酸菌进行了筛选和鉴定。采用MR S培养平板从泡菜中筛选出99株接触酶试验为阴性、革兰氏染色为阳性、有明显透明钙圈的菌株,并分别测定它们的降胆固醇作用。胆固醇降解率大于30%的有8株,其中I4的降解率为37.3%±1.50%(n=3)。通过生理生化试验和16S rRNA 序列分析法,鉴定菌株I4为植物乳杆菌(Lactobacillus plantarum )。对植物乳杆菌I4进行了耐酸、耐胆盐试验的研究,实验表明植物乳杆菌I4具有很好的耐酸能力和一定的耐胆盐能力。对降胆固醇植物乳杆菌I4菌制剂在发酵泡菜中的应用进行研究。实验表明:与未添加I4菌种的发酵泡菜对比,泡菜成熟时间缩短,亚硝酸盐含量降低,总酸与总氨基态氮没有差异,感官检验没有差异,并进行了微生物指标测定,符合国家GB 2714-2003《酱腌菜卫生标准》。%A strain of lactobacillus with cholesterol-reducing ability is screened and identified.Use MRS Plate to screen 99 strains from pickles with catalase test being negative,Gram staining being positive and obvious transparent calcium circle and their cholesterol-reducing effect is determined, wherein there are 8 strains with cholesterol degradation rate greater than 30%,the degradation rate of I4 is 37.3%±1.50% (n= 3).Through physiological and biochemical test and 16S rRNA sequence analysis,the strain I4 is identified as Lactobacillus plantarum. The acid resistance and bile salt resistance tests for I4 are carried out;the experimental results show that Lactobacillus plantarum I4 has good acid resistance ability and certain bile salt resistance ability.The application of Lactobacillus plantarum I4 in fermented pickles is studied.The experimental results show that comparison offermented pickles without I4 species,the mature time of pickles is shortened,the nitrite content is reduced,the total acids and total amino nitrogen have no differences,and there is no difference with sensory test,and the microbial indexes are determined,which is accorded with the national standard GB 2714-2003.

  15. Efecte de la incorporació de biochar al sòl i estrès abiòitic de contaminants orgànics emergents presents en l'aigua de reg en la producció de biomassa de Lactuca sativa

    Nadal Talavera, Marta


    Recently, the hydrological demand has increased while the supply has stayed the same or even, in some places, it has diminished. One possible solution to this problem is the use of recycled wastewater, mostly in places with scarcity of water. The main use of this water is in the agriculture and landscape restoration. However, the wastewaters treatment plants used for the water reclamation are not able to eliminate the presence of xenobiotics and, among them, the presence of emergent organic c...

  16. Análisis genómico de girasol: desarrollo de colecciones de ESTs y de una plataforma bioinformática para estudios de expresión de genes candidato en respuestas a estreses abióticos

    Fernández, Paula del Carmen


    El girasol es uno de los principales cultivos oleaginosos del mundo por su volumen de producción y composición en ácidos grasos insaturados. En promedio, durante los últimos cinco años, la producción mundial se ha mantenido estable concentrándose el 72% de la misma entre Argentina y la Unión Europea. A pesar de esto y debido a las condiciones de los precios internacionales y la productividad comparativa con otros cultivos, el área de producción se está desplazando a regiones más áridas a nive...

  17. Relation between chemical composition of Grateloupia doryphora (Montagne Howe, Gymnogongrus griffithsiae (Turner Martius, and abiotic parameters Relação entre composição química de Grateloupia doryphora (Montagne Howe, Gymnogongrus griffithsiae (Turner Martius e parâmetros abióticos

    Paulo Nelo Medeiros Perfeto


    Full Text Available In Grateloupia doryphora and Gymnogongrus griffithsiae the seasonal variation of their chemical compounds was studied, establishing a relation with the physical and chemical properties of seawater. High values of proteins in the studied species were detected during the winter, 28.88% in G. doryphora and 26.68% in G. griffithsiae, corresponding to the maximum period of ammonium concentration in the marine environment. The variation in carbohydrates content showed an inverse relation with the proteins, with a maximum of 54.72% in G. doryphora and of 55.36% in G. griffithsiae, both in summer, positively correlated with salinity and temperature of sea water. Lipids content was low in both species. In G. doryphora the values ranged between 0.81 and 1.30% and, in G. griffithsiae, from 0.71 to 1.50% of dry weight, showing a direct relation with the amount of nitrogen in the seawater. The maximum content of ashes, phosphorus and potassium occurred in autumn and winter, respectively, with 11.85%, 0.20%, 1.27% in G. doryphora, and 14.46%, 0.14%, 1.41% in G. griffithsiae.Foi estudado em Grateloupia doryphora e Gymnogongrus griffithsiae a variação sazonal de seus compostos químicos, estabelecendo uma relação com os parâmetros físico-químicos da água do mar. Durante o inverno foram detectados valores altos de proteína nas espécies estudadas, com 28,88 % em G. doryphora e 26,68 % em G. griffithsiae, correspondendo ao período de concentração máxima de amônio no ambiente marinho. A variação no conteúdo de carboidratos apresentou relação inversa à de proteínas. Os valores máximos de 54,72 % em G. doryphora e de 55,36 % em G. griffithsiae, ocorreram no verão, correlacionados positivamente com salinidade e temperatura da água do mar. Os conteúdos de lipídeos foram baixos em ambas as espécies. Em G. doryphora os valores variaram entre 0,81 e 1,30 % e em G. griffithsiae, de 0,71 a 1,50 % do peso seco, mostrando relação direta com a quantidade de nitrogênio na água do mar. O conteúdo máximo de cinzas, fósforo e potássio ocorreu no outono e inverno, respectivamente com 11,85 %, 0,20 % e 1,27 % em G. doryphora e 14,46 %, 0,14 % e 1,41 % em G. griffithsiae.

  18. Relation between chemical composition of Grateloupia doryphora (Montagne) Howe, Gymnogongrus griffithsiae (Turner) Martius, and abiotic parameters Relação entre composição química de Grateloupia doryphora (Montagne) Howe, Gymnogongrus griffithsiae (Turner) Martius e parâmetros abióticos

    Paulo Nelo Medeiros Perfeto


    In Grateloupia doryphora and Gymnogongrus griffithsiae the seasonal variation of their chemical compounds was studied, establishing a relation with the physical and chemical properties of seawater. High values of proteins in the studied species were detected during the winter, 28.88% in G. doryphora and 26.68% in G. griffithsiae, corresponding to the maximum period of ammonium concentration in the marine environment. The variation in carbohydrates content showed an inverse relation with the p...

  19. Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops Species Análisis de la Expresión Génica: Una Forma de Estudiar la Tolerancia a Estreses Abióticos en Cultivos

    Eduardo Pérez-Torres


    Full Text Available Regions traditionally destined to agriculture report an ever increasing exposure to cold and drought conditions. This is especially important in countries like Chile where crop management options are limited. The development of new cultivars with better yields under adverse conditions is fundamental if the ever increasing demand for food is to be matched; however, improving tolerance to abiotic stresses has proved to be a complex task. In this regard, development in plant physiology and genomics in the last 20 years has led to a deeper understanding of how plants respond to stress and mechanisms responsible for different ranges of tolerance observed in nature. This review discusses the techniques currently most in use in gene expression analysis, together with some important experimental design variables, such as the developmental stage of the plant, stress intensity and duration, and how different stresses may interact when performing assays. On the other hand, it is fundamental to properly select gene expression techniques according to the available information on the genome, the crop and the final objective of the research. All these points must be considered to ease transition from genomics to practical applications to crop species in order to increase their tolerance to stress. In this regard, the rapid development of new techniques in gene expression analysis with lower costs will determine a new revolution in crop research in coming decades. Therefore, Chile needs to be prepared in this area to continue its development as a major food producer worldwide.Las regiones agrícolas están cada vez más expuestas a condiciones de frío y sequía, algo especialmente importante en países con opciones limitadas de manejo de cultivos como Chile. Si la creciente demanda por alimento ha de ser cubierta, es necesaria la compleja tarea del desarrollo de nuevos cultivares con mejores rendimientos bajo condiciones de estrés. El desarrollo de la fisiología vegetal y la genómica en los últimos 20 años ha permitido entender mejor cómo las plantas responden al estrés y los mecanismos responsables de los distintos rangos de tolerancia observados en la naturaleza. En esta revisión, se discuten las técnicas más usadas actualmente en análisis de expresión génica y algunas variables que deben ser consideradas en el diseño experimental tales como el estado de desarrollo de la planta y la intensidad, duración e interacción de distintos tipos de estrés, además de la elección de técnicas apropiadas de acuerdo a la información disponible del genoma del cultivo y el objetivo final de la investigación. Todos estos puntos son fundamentales para facilitar la transición desde la genómica a aplicaciones prácticas en el aumento de la tolerancia al estrés de los cultivos. En este sentido, el rápido desarrollo de nuevas técnicas para estudiar la expresión de genes a menor costo determinará una nueva revolución en la investigación de cultivos en las próximas décadas. En este sentido, Chile necesita estar preparado en esta área para continuar su desarrollo como un importante productor de alimentos a nivel mundial.

  20. 新媒体环境下高校辅导员职业能力提升的路径选择%On What CoIIege Advisors ShouId Do to Upgrade Their OccupationaI AbiIity in the New Media Environment

    岳甜; 范高社; 杨华


    新媒体环境下高校辅导员群体因教育对象、教育环境及教育主体自身发生的深刻变化,亟待提升符合新媒体要求的沟通表达能力、传播主流价值观的理论教育能力、面向实践提炼问题的调查研究能力,构建更为全面的高校辅导员职业能力体系,实现从工作内容、工作实践、角色定位到培养方式、评价体系等方面的观念“转变”。%As the educational objects ,educational environment and the educational main body itself un-dergo dramatic change in the New Media environment ,college advisors urgently need to upgrade their communicative ability in order to meet the requirements of the New Media ,their theoretical education ability in order to spread mainstream values and their investigation and research ability to identify problems in practice .A comprehensive and complete college advisors occupational ability system calls for conceptual change in aspects from job content ,working practice ,role definition to training mode and evaluation system .

  1. ABSCISIC ACID-INSENSITIVE 4 negatively regulates flowering through directly promoting Arabidopsis FLOWERING LOCUS C transcription

    Shu, Kai; Chen, Qian; Wu, Yaorong; Liu, Ruijun; Zhang, Huawei; Wang, Shengfu; Tang, Sanyuan; Yang, Wenyu; Xie, Qi


    During the life cycle of a plant, one of the major biological processes is the transition from the vegetative to the reproductive stage. In Arabidopsis, flowering time is precisely controlled by extensive environmental and internal cues. Gibberellins (GAs) promote flowering, while abscisic acid (ABA) is considered as a flowering suppressor. However, the detailed mechanism through which ABA inhibits the floral transition is poorly understood. Here, we report that ABSCISIC ACID-INSENSITIVE 4 (ABI4), a key component in the ABA signalling pathway, negatively regulates floral transition by directly promoting FLOWERING LOCUS C (FLC) transcription. The abi4 mutant showed the early flowering phenotype whereas ABI4-overexpressing (OE-ABI4) plants had delayed floral transition. Consistently, quantitative reverse transcription–PCR (qRT–PCR) assay revealed that the FLC transcription level was down-regulated in abi4, but up-regulated in OE-ABI4. The change in FT level was consistent with the pattern of FLC expression. Chromatin immunoprecipitation-qPCR (ChIP-qPCR), electrophoretic mobility shift assay (EMSA), and tobacco transient expression analysis showed that ABI4 promotes FLC expression by directly binding to its promoter. Genetic analysis demonstrated that OE-ABI4::flc-3 could not alter the flc-3 phenotype. OE-FLC::abi4 showed a markedly delayed flowering phenotype, which mimicked OE-FLC::WT, and suggested that ABI4 acts upstream of FLC in the same genetic pathway. Taken together, these findings suggest that ABA inhibits the floral transition by activating FLC transcription through ABI4. PMID:26507894


    Full Text Available ABREDISTBBNNAPA S000262 16-Feb-2001 (last modified) seki dist B (distal portion of ...equired for seed specific expression and ABA responsiveness; See S000263, S000264; dist B ABRE mediated tran...sactivation by ABI3 adn ABI3-dependent response to ABA; a tetramer of the composite RY/G complex medi

  3. Eksperdid: video Liibanonis röövitud eestlastest on hea märk / Kärt Anvelt, Kadri Ibrus, Erik Rand

    Anvelt, Kärt, 1973-


    Youtube'i videokeskkonda ilmus Liibanonis röövitud eestlastest video, milles nad paluvad Liibanoni, Saudi Araabia, Jordaania ja Prantsusmaa juhtide abi enda vabastamiseks. Saudi Araabia, Araabia Ühendemiraadid ja Jordaania on lubanud igakülgset abi. President Toomas Hendrik Ilvese avaldusest seoses videopöördumisega

  4. Impact of Experimentally Elevated Ozone on Seed Germination and Growth of Russian Pine (Pinus sylvestris) and Spruce (Picea spp.) Provenances

    Prozherina, Nadezda; Nakvasina, Elena; Oksanen, Elina


    The impact of elevated ozone concentrations on early ontogenetic stages of pine (Pinus sylvestris) and spruce (Picea abies, Picea obovata, P. abies X P. obovata) seedlings originating from different provenances in Russia were studied in the open-field ozone fumigation system located in Kuopio, Finla

  5. Vaidlus Soomes: miks me peaksime aitama Iirimaad? / Tiina Hakman

    Hakman, Tiina


    Soomes on opositsioon Iirimaale abi andmise vastu, ka ajalehtede lugejaküsitlused ja kommentaarid näitavad ülekaalukalt eitavat suhtumist abi andmisse. Soome rahandusminister ja peaminister veenavad rahvast vajaduses lüüa kaasa Iirimaa abipaketis

  6. AcEST: BP914988 [AcEST

    Full Text Available ctase OS=Delftia acido... 37 0.081 sp|Q9M7D0|TPSD9_ABIGR Terpinolene synthase, chloroplastic OS=Abi... 31 4....LERLQELRDS 210 >sp|Q9M7D0|TPSD9_ABIGR Terpinolene synthase, chloroplastic OS=Abies grandis GN=ag9 PE=1 SV=1

  7. Dicty_cDB: SHB712 [Dicty_cDB

    Full Text Available 52 0.030 1 CO731046 |CO731046.1 JBW004G03.b_019.abi Pineapple green mature fruit ...78 |CO730778.1 JBW001D09.b_073.abi Pineapple green mature fruit cDNA library Ananas comosus cDNA clone JBW00

  8. Dicty_cDB: SHB341 [Dicty_cDB

    Full Text Available 250F14 from 4, complete sequence. 52 0.031 1 CO731046 |CO731046.1 JBW004G03.b_019.abi Pineapple green mature... CO730778 |CO730778.1 JBW001D09.b_073.abi Pineapple green mature fruit cDNA libra

  9. Dicty_cDB: Contig-U16299-1 [Dicty_cDB

    Full Text Available 43 ) JBW089C03.b_027.abi Pineapple week 5-10 nematode-... 139 1e-36 4 ( CT910186 ) Phaeodactylum tricornutum...e-31 2 ( D35256 ) Caenorhabditis elegans cDNA clone yk19a6 : 5' end, ... 92 5e-31 3 ( DT338842 ) JBW090D11.b_089.abi Pineapple

  10. Genetic determinants of the ankle-brachial index

    Wassel, Christina L; Lamina, Claudia; Nambi, Vijay


    Candidate gene association studies for peripheral artery disease (PAD), including subclinical disease assessed with the ankle-brachial index (ABI), have been limited by the modest number of genes examined. We conducted a two stage meta-analysis of ∼50,000 SNPs across ∼2100 candidate genes to iden...... to identify genetic variants for ABI....

  11. 75 FR 12483 - Courtesy Notice of Liquidation


    ... file their entry summaries: Importers of record and customs brokers who are duly authorized agents of... filed in ABI. The ABI filer, who is either the importer of record or a customs broker, already receives... SECURITY Bureau of Customs and Border Protection DEPARTMENT OF THE TREASURY 19 CFR Part 159 RIN...

  12. Patients with severe acquired brain injury show increased arousal in tilt-table training

    Riberholt, Christian G; Thorlund, Jonas Bloch; Mehlsen, Jesper;


    Patients with severe acquired brain injury (ABI) are often mobilised using a tilt-table. Complications such as orthostatic intolerance have been reported. The primary objective of this study was to investigate if using a tilt-table was feasible for mobilising patients with severe ABI admitted...

  13. Evaluation of a Reading Comprehension Strategy Package to Improve Reading Comprehension of Adult College Students with Acquired Brain Injuries

    Griffiths, Gina G.


    Adults with mild to moderate acquired brain injury (ABI) often pursue post-secondary or professional education after their injuries in order to enter or re-enter the job market. An increasing number of these adults report problems with reading-to-learn. The problem is particularly concerning given the growing population of adult survivors of ABI.…

  14. Dutch Multifactor Fatigue Scale : A New Scale to Measure the Different Aspects of Fatigue After Acquired Brain Injury

    Visser-Keizer, Annemarie C.; Hogenkamp, Antoinette; Westerhof-Evers, Herma J.; Egberink, Iris J. L.; Spikman, Jacoba M.


    Objectives: To develop the Dutch Multifactor Fatigue Scale (DMFS), a new scale to assess the nature and impact of fatigue and coping with fatigue in the chronic phase after acquired brain injury (ABI) and to analyze the psychometric properties of this scale in a mixed group of patients with ABI. Des

  15. Front-office/back-office configurations and operational performance in complex health services

    Gemmel, P.; van Steenis, T.; Meijboom, B.R.


    BACKGROUND: Acquired brain injury (ABI) occurs from various causes at different ages and leads to many different types of healthcare needs. Several Dutch ABI-networks installed a local co-ordination and contact point (CCP) which functions as a central and easily accessible service for people to cons

  16. Activity-Based Intervention Practices in Special Education

    Ozen, Arzu; Ergenekon, Yasemin


    Teaching practices in natural settings such as activity-based intervention (ABI) are suggested as alternatives to be used in effective early childhood education. As a multidisciplinary model, ABI consists of four components, which are choosing activities according to the child's interests; teaching generalizable goals embedded in routines and…

  17. NCBI nr-aa BLAST: CBRC-DSIM-04-0030 [SEVENS

    Full Text Available CBRC-DSIM-04-0030 ref|NP_001011594.1| G-protein coupled receptor [Apis mellifera] e...mb|CAB76374.1| G-protein coupled receptor [Apis mellifera] gb|ABI94393.1| tyramine receptor [Apis mellifera]... gb|ABI94394.1| tyramine receptor [Apis mellifera] NP_001011594.1 2e-61 36% ...

  18. HSPC300 and its role in neuronal connectivity.

    Qurashi, A.; Sahin, H.B.; Carrera, P.; Gautreau, A.; Schenck, A.; Giangrande, A.


    ABSTRACT: BACKGROUND: The WAVE/SCAR complex, consisting of CYFIP (PIR121 or Sra1), Kette (Nap1), Abi, SCAR (WAVE) and HSPC300, is known to regulate the actin nucleating Arp2/3 complex in a Rac1-dependent manner. While in vitro and in vivo studies have demonstrated that CYFIP, Kette, Abi and SCAR wor

  19. Arabidopsis CDS blastp result: AK111828 [KOME

    Full Text Available AK111828 J023064H19 At5g61380.1 ABI3-interacting protein 1 (AIP1) identical to pseu...:9247019; contains Pfam profile PF00072: Response regulator receiver domain; identical to cDNA ABI3-interacting protein 1 (aip1 gene) GI:6996312 9e-73 ...


    Full Text Available Arabidopsis (A.T.) homeodomain-leucine zipper protein, ATHB6; ATHB6 is a target of the protein phosphatase ...ABI1 and regulates hormone responses; See S000371; ATHB6; ABA; ABI1; homeodomain-leucine zipper; Arabidopsis thaliana CAATTATTA ... ...ATHB6COREAT S000399 27-Aug-2002 (last modified) uchi Consensus binding sequence for

  1. Comparative study of cogasification and co-combustion of communal sewage sludge in brown coal fuelled plants; Vergleich der Mitvergasung und Mitverbrennung kommunaler Klaerschlaemme in braunkohlegefeuerten Anlagen

    Schiffer, H.P.; Bierbaum, K.; Adlhoch, W.; Thomas, G. [Rheinbraun AG, Koeln (Germany)


    Co-combustion and cogasification of sewage sludge in brown coal fuelled plants are compared, and an economic assessment is made. (ABI) [Deutsch] Die Mitverbrennung und Mitvergasung von Klaerschlamm in braunkohlegefeuerten Anlagen werden verglichen und auf ihre Wirtschaftlichkeit hin untersucht. (ABI)

  2. Ankle-brachial pressure index estimated by laser Doppler in patients suffering from peripheral arterial obstructive disease.

    Ludyga, Tomasz; Kuczmik, Waclaw B; Kazibudzki, Marek; Nowakowski, Przemyslaw; Orawczyk, Tomasz; Glanowski, Michal; Kucharzewski, Marcin; Ziaja, Damion; Szaniewski, Krzysztof; Ziaja, Krzysztof


    Ankle-brachial index (ABI) measurements are widely used for evaluating the functional state of circulation in the lower limbs. However, there is some evidence that the value of ABI does not accurately reflect the degree of walking impairment in symptomatic patients with peripheral arterial obstructive disease (PAOD). We investigated the diagnostic value of ABI estimated by means of laser Doppler flowmetry (IT) for evaluating limb ischemia. We wanted to know whether laser Doppler could be more sensitive than the Doppler method in predicting walking capacity in patients with stable intermittent claudication. We analyzed a group of 30 patients with intermittent claudication (Fontain II, II/III) who were admitted for reconstructive treatment. There were 21 men and 9 women, aged 46-74 (mean 61) years. All patients underwent the treadmill test, and pain-free walking distances were measured. In each patient, we measured ABI using the two different methods: Doppler ultrasound device (ABI-Doppler) and laser Doppler (ABI-laser Doppler). The claudication distances were 25-200 m (mean 73 +/- 50.2 m). ABI-Doppler was 0.2-0.7 (0.582 +/- 0.195). ABI-laser Doppler measurements were 0.581 (+/-0.218). A correlation was found between ABI-Doppler and claudication distance (r = 0.46, P = 0.009). Also, ABI-laser Doppler values significantly correlated with claudication distances (r = 0.536, P = 0.002). The ABI evaluated by laser Doppler correlated well with claudication distances in patients with PAOD. Comparison of Doppler and laser Doppler measurements used for determining ABI showed that both methods have similar predictive power for walking capacity; however, higher correlation was observed between claudication distances and ABI measured by laser Doppler flowmetry. ABI-laser Doppler measurements are easier, are quicker, and seem to be better suited for noncompliant patients. Further investigation should be undertaken to determine whether laser Doppler is superior to the Doppler

  3. Phage abortive infection in lactococci: variations on a theme.

    Chopin, Marie-Christine; Chopin, Alain; Bidnenko, Elena


    Abortive infection (Abi) systems, also called phage exclusion, block phage multiplication and cause premature bacterial cell death upon phage infection. This decreases the number of progeny particles and limits their spread to other cells allowing the bacterial population to survive. Twenty Abi systems have been isolated in Lactococcus lactis, a bacterium used in cheese-making fermentation processes, where phage attacks are of economical importance. Recent insights in their expression and mode of action indicate that, behind diverse phenotypic and molecular effects, lactococcal Abis share common traits with the well-studied Escherichia coli systems Lit and Prr. Abis are widespread in bacteria, and recent analysis indicates that Abis might have additional roles other than conferring phage resistance.

  4. Incidence of butt rot in a tree species experiment in northern Denmark

    Ronnberg, J; Vollbrecht, G.; Thomsen, Iben Margrete


    The susceptibility to infections by Heterobasidion annosum (Fr.) Bref. was examined in a 28-year-old tree species experiment in northern Jutland, Denmark. Silver fir (Abies alba Mill.), Caucasian fir (Abies nordmanniana (Stev.) Spach), grand fir (Abies grandis (Dougl) Lindl.), noble fir (Abies...... procera Rehd.), Japanese larch (Larix kaempferi (Lamb.) Carr.), Norway spruce (Picea abies (L.) Karst.), Sitka spruce (Picea sitchensis (Bong.) Carr.), and Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) were planted after shelterwood felling of a mountain pine (Pinus uncinata Mill. ex Mirb.) stand...... that had been infected by H. annosum. Douglas fir and noble fir showed the greatest mortality due to H. annosum during the first 5 years after planting. At first thinning the highest incidences of butt rot were recorded in noble fir, Japanese larch and Sitka spruce, with 44%, 43% and 36% of the thinned...

  5. Comparison of ankle-brachial index measured by an automated oscillometric apparatus with that by standard Doppler technique in vascular patients

    Korno, M.; Eldrup, N.; Sillesen, H.


    OBJECTIVES: To evaluate the determination of ankle-brachial indices (ABIs) using a simple automated ankle pressure measurement device in comparison with the Doppler technique. DESIGN: ABI was measured in 61 patients (122 legs) admitted to the department of vascular surgery, Rigshospitalet. ABI...... was calculated twice using both the methods on both legs. MATERIALS AND METHODS: We tested the automated oscillometric blood pressure device, CASMED 740, for measuring ankle and arm blood pressure and compared it with the current gold standard, the hand-held Doppler technique, by the Bland-Altman analysis....... RESULTS: Using the Doppler-derived ABI as the gold standard, the sensitivity and specificity of the oscillometric method for determining an ABI Udgivelsesdato: 2009/11...

  6. Sex differences in the prevalence and clinical outcomes of subclinical peripheral artery disease in the Health, Aging, and Body Composition (Health ABC) study.

    Hiramoto, Jade S; Katz, Ronit; Ix, Joachim H; Wassel, Christina; Rodondi, Nicolas; Windham, B Gwen; Harris, Tamara; Koster, Annemarie; Satterfield, Suzanne; Newman, Anne; Shlipak, Michael G


    The objective of the study was to determine if there are sex-based differences in the prevalence and clinical outcomes of subclinical peripheral artery disease (PAD). We evaluated the sex-specific associations of ankle-brachial index (ABI) with clinical cardiovascular disease outcomes in 2797 participants without prevalent clinical PAD and with a baseline ABI measurement in the Health, Aging, and Body Composition study. The mean age was 74 years, 40% were black, and 52% were women. Median follow-up was 9.37 years. Women had a similar prevalence of ABI prevalence of ABI 0.9-1.0 (15% versus 10%, respectively; P disease (CHD) mortality, incident clinical PAD and incident myocardial infarction in both women and men. ABI prevalence of subclinical PAD.

  7. Serum phosphorus levels and the spectrum of ankle-brachial index in older men: the Osteoporotic Fractures in Men (MrOS) study.

    Meng, Jerry; Wassel, Christina L; Kestenbaum, Bryan R; Collins, Tracie C; Criqui, Michael H; Lewis, Cora E; Cummings, Steve R; Ix, Joachim H


    A higher serum phosphorus level is associated with cardiovascular disease (CVD) events among community-living populations. Mechanisms are unknown. The ankle-brachial index (ABI) provides information on both atherosclerosis and arterial stiffness. In this cross-sectional study (2000-2002), the authors evaluated the association of serum phosphorus levels with low ( or =1.40 or incompressible) ABI as compared with intermediate ABI in 5,330 older US men, among whom the mean serum phosphorus level was 3.2 mg/dL (standard deviation, 0.4), 6% had a low ABI, and 5% had a high ABI. Each 1-mg/dL increase in serum phosphorus level was associated with a 1.6-fold greater prevalence of low ABI (95% confidence interval (CI): 1.2, 2.1; P < 0.001) and a 1.4-fold greater prevalence of high ABI (95% CI: 1.0, 1.9; P = 0.03) in models adjusted for demographic factors, traditional CVD risk factors, and kidney function. However, the association of phosphorus with high ABI differed by chronic kidney disease (CKD) status (in persons with CKD, prevalence ratio = 2.96, 95% CI: 1.61, 5.45; in persons without CKD, prevalence ratio = 1.14, 95% CI: 0.81, 1.61; interaction P = 0.04). In conclusion, among community-living older men, higher phosphorus levels are associated with low ABI and are also associated with high ABI in persons with CKD. These associations may explain the link between serum phosphorus levels and CVD events.

  8. Ophioninae (Hymenoptera: Ichneumonidae wasp community in the cloudy forest Monteseco, Cajamarca, Peru

    Evelyn Sánchez


    Full Text Available We describe the species composition of the subfamily Ophioninae (Hymenoptera: Ichneumonidae along an altitudinal gradient in the cloudy forest Monteseco, Cajamarca, Peru collected in 2009 and 2010. Eighteen species were recorded in three genera of Ophioninae: Alophophion, Enicospilus y Ophion. Five species are recorded for the first time in Peru: Ophion polyhymniae Gauld, 1988; Enicospilus cubensis (Norton, 1863; E. guatemalensis (Cameron, 1886; E. cressoni Hooker, 1912 y E. mexicanus (Cresson, 1874. Subfamily composition varies with the elevation. The highest species richness (S=11 was found at 2150 m and the lowest (S=3 at 3116 m. Enicospilus is more diverse from low to mid elevation, Ophion from mid to high elevation and Alophophion occurs predominantly at high elevation.

  9. Genetic Structure and Selection of a Core Collection for Long Term Conservation of Avocado in Mexico

    Guzmán, Luis F.; Machida-Hirano, Ryoko; Borrayo, Ernesto; Cortés-Cruz, Moisés; Espíndola-Barquera, María del Carmen; Heredia García, Elena


    Mexico, as the center of origin of avocado (Persea americama Mill.), harbors a wide genetic diversity of this species, whose identification may provide the grounds to not only understand its unique population structure and domestication history, but also inform the efforts aimed at its conservation. Although molecular characterization of cultivated avocado germplasm has been studied by several research groups, this had not been the case in Mexico. In order to elucidate the genetic structure of avocado in Mexico and the sustainable use of its genetic resources, 318 avocado accessions conserved in the germplasm collection in the National Avocado Genebank were analyzed using 28 markers [9 expressed sequence tag-Simple Sequence Repeats (SSRs) and 19 genomic SSRs]. Deviation from Hardy Weinberg Equilibrium and high inter-locus linkage disequilibrium were observed especially in drymifolia, and guatemalensis. Total averages of the observed and expected heterozygosity were 0.59 and 0.75, respectively. Although clear genetic differentiation was not observed among 3 botanical races: americana, drymifolia, and guatemalensis, the analyzed Mexican population can be classified into two groups that correspond to two different ecological regions. We developed a core-collection by K-means clustering method. The selected 36 individuals as core-collection successfully represented more than 80% of total alleles and showed heterozygosity values equal to or higher than those of the original collection, despite its constituting slightly more than 10% of the latter. Accessions selected as members of the core collection have now become candidates to be introduced in cryopreservation implying a minimum loss of genetic diversity and a back-up for existing field collections of such important genetic resources. PMID:28286510

  10. Perceptions of the ankle brachial index amongst podiatrists registered in Western Australia

    Chen Pamela Y


    Full Text Available Abstract Background The ankle brachial index (ABI is an objective diagnostic tool that is widely used for the diagnosis of peripheral arterial disease. Despite its usefulness, it is evident within the literature that many practitioners forgo using this screening tool due to limiting factors such as time. There is also no recommended technique for ABI measurement. The purpose of this study is to investigate the perceptions of the use of ABI clinically among Western Australian podiatrists. Methods This study was a cross sectional survey which evaluated the perceptions of the ABI amongst registered podiatrists in Western Australia. The study sample was obtained from the register of podiatrists listed with the Podiatrists Registration Board of Western Australia. Podiatrists were contacted by telephone and invited to participate in a telephone questionnaire. Chi-square tests were performed to determine if there was a statistically significant relationship between use of the ABI and podiatrists’ profile which included: sector of employment; geographical location; and length of time in practice. Results There is a statistically significant relationship (p=0.004 between podiatrists’ profile and the use of ABI, with higher usage in the tertiary hospital setting than in private practice. Length of time spent in practice had no significant impact on ABI usage (p=0.098. Time constraints and lack of equipment were key limiting factors to performing the ABI, and no preferred technique was indicated. Conclusion Western Australian podiatrists agree that the ABI is a useful tool for lower limb vascular assessment, however, various factors influence uptake in the clinical setting. This study suggests that a podiatrists’ profile has a significant influence on the use of the ABI, which may be attributed to different patient types across the various settings. The influence of time spent in practice on ABI usage may be attributed to differences in clinical

  11. Low prevalence of peripheral arterial disease in a cross-sectional study of Danish HIV-infected patients

    Knudsen, Andreas; Malmberg, Catarina Anna Evelina; Kjær, Andreas


    . However, controversies exist on the prevalence of PAD among HIV-infected patients. In this study we aimed to measure the prevalence of PAD among HIV-infected patients and compare the ABI with carotid intima-media thickness (cIMT) and other known CVD risk predictors. METHODS: We prospectively included HIV...... patients included (mean age 52 years, 75% male, 94% receiving antiretroviral therapy (ART), 33% active smokers), 1 had a pre-exercise ABI ≤ 0.9 and in addition 3 patients had a post-exercise ABI ≤ 0.9. We found a poor correlation between ABI and traditional CVD risk factors other than body mass index....... In contrast, a strong correlation was found between cIMT and traditional risk factors. Values of post-exercise ABI and cIMT were not correlated. The current ART did not influence ABI values. CONCLUSIONS: We found a low prevalence of PAD in HIV-infected patients. ABI did not correlate with CVD risk factors...

  12. Basic Pentacysteine Proteins Repress Abscisic Acid Insensitive4 Expression via Direct Recruitment of the Polycomb-Repressive Complex 2 in Arabidopsis Root Development.

    Mu, Ying; Zou, Meijuan; Sun, Xuwu; He, Baoye; Xu, Xiumei; Liu, Yini; Zhang, Lixin; Chi, Wei


    Plant transcription factors generally act in complex regulatory networks that function at multiple levels to govern plant developmental programs. Dissection of the interconnections among different classes of transcription factors can elucidate these regulatory networks and thus improve our understanding of plant development. Here, we investigated the molecular and functional relationships of the transcription factors ABSCISIC ACID INSENSITIVE 4 (ABI4) and members of the BASIC PENTACYSTEINE (BPC) family in lateral root (LR) development of Arabidopsis thaliana Genetic analysis showed that BPCs promote LR development by repressing ABI4 expression. Molecular analysis showed that BPCs bind to the ABI4 promoter and repress ABI4 transcription in roots. BPCs directly recruit the Polycomb Repressive Complex 2 (PRC2) to the ABI4 locus and epigenetically repress ABI4 expression by catalyzing the trimethylation of histone H3 at lysine 27. In addition, BPCs and ABI4 coordinate their activities to fine-tune the levels of PIN-FORMED1, a component of the auxin signaling pathway, and thus modulate LR formation. These results establish a functional relationship between two universal and multiple-role transcription factors and provide insight into the mechanisms of the transcriptional regulatory networks that affect Arabidopsis organogenesis.

  13. Ankle-Brachial Index: Nurses Strategy To Cardiovascular Disease Risk Factors Identification

    Daniela Luisa Maggi


    Full Text Available Elevated risk of fatal and non-fatal cardiovascular events is associated with high prevalence of peripheral arterial disease, with assessment through the ankle-brachial index (ABI. This study aimed to demonstrate that the ABI and the Edinburgh Claudication Questionnaire are tools to be used by nurses in prevention and/or treatment of CVD (cardiovascular disease. A cross-sectional study was carried out with patients from a cardiovascular clinic. The Edinburgh Claudication Questionnaire was applied and the ABI was measured with the formula (ABI= Blood Pressure Ankle/Blood Pressure Brachial. A total of 115 patients were included, most were females (57.4%, aged 60.6 ± 12.5 years. The most prevalent risk factors were hypertension (64.3%, physical inactivity (48.7% and family history (58.3%. The study showed that abnormal ABI was frequently found and 42.6% of the patients with abnormal ABI showed intermittent claudication. The method to evaluate the ABI associated to the Edinburg Claudication Questionnaire, can be easily used by nurses in the clinical evaluation of asymptomatic and symptomatic CVD patients.

  14. Susceptibility to low-temperature photoinhibition in three conifers differing in successional status.

    Robakowski, Piotr


    Susceptibility to photoinhibition of the evergreen conifers Abies alba Mill., Picea abies (L.) Karst. and Pinus mugo Turra was investigated in an unheated greenhouse during winter and spring 2003. Photosynthetic performance of the seedlings was assessed by chlorophyll a fluorescence and analyses of chlorophyll and total carotenoid concentrations in needles. During winter months, maximum quantum yield of PSII photochemistry (ratio of variable to maximum fluorescence, Fv/Fm) was significantly greater in A. alba than in P. abies and P. mugo. Abies alba also sustained higher maximum apparent electron transport rate (ETRmax) than P. abies and P. mugo. Total concentrations of chlorophyll and carotenoids in needles decreased during the winter in P. mugo and P. abies, but remained stable in A. alba. For all species, Fv/Fm decreased from December until February and then increased to a maximum in April. Photoinhibition was greatest (Fv/Fm mugo. Winter photoinhibition in P. abies, P. mugo and, to a lesser extent, in A. alba may reflect adaptive photoprotection of the photosynthetic apparatus in winter.

  15. Associations Between Ankle-Brachial Index and Cognitive Function: Results from the Lifestyle Interventions and Independence for Elders Trial

    Espeland, Mark A.; Newman, Anne B.; Sink, Kaycee; Gill, Thomas M.; King, Abby C.; Miller, Michael E.; Guralnik, Jack; Katula, Jeff; Church, Timothy; Manini, Todd; Reid, Kieran F.; McDermott, Mary M.


    OBJECTIVE To evaluate cross-sectional and longitudinal associations between ankle-brachial index (ABI) and indicators of cognitive function DESIGN Randomized clinical trial (Lifestyle Interventions and Independence for Elders Trial) SETTING Eight US academic centers PARTICIPANTS 1,601 adults (ages 70–89 years, sedentary, non-demented, and with functional limitations MEASUREMENTS Baseline ABI and interviewer- and computer-administered cognitive function assessments were obtained from which compared a physical activity intervention with a health education control. Cognitive function was re-assessed 24 months later (interviewer-administered) and 18 or 30 months later (computer-administered) and central adjudication was used to classify individuals as having mild cognitive impairment, probable dementia, or neither. RESULTS Lower ABI had a modest independent association poorer cognitive functioning at baseline (partial r=0.09; p<0.001). While, lower baseline ABI was not associated with overall changes in cognitive function test scores, it was associated with higher odds for two-year progression to a composite of either mild cognitive impairment or probable dementia (OR=2.60 per unit lower ABI; 95% confidence interval [1.06,6.37]). Across two years, changes in ABI were not associated with changes in cognitive function. CONCLUSION In an older cohort of non-demented sedentary individuals with functional limitations, lower baseline ABI was independently correlated with cognitive function and associated with greater 2-year risk for progression to mild cognitive impairment or probable dementia. PMID:25869993

  16. Correlation between Patient-Reported Symptoms and Ankle-Brachial Index after Revascularization for Peripheral Arterial Disease

    Hyung Gon Je


    Full Text Available Improvement in quality of life (QoL is a primary treatment goal for patients with peripheral arterial disease (PAD. The current study aimed to quantify improvement in the health status of PAD patients following peripheral revascularization using the peripheral artery questionnaire (PAQ and ankle-brachial index (ABI, and to evaluate possible correlation between the two methods. The PAQ and ABI were assessed in 149 symptomatic PAD patients before, and three months after peripheral revascularization. Mean PAQ summary scores improved significantly three months after revascularization (+49.3 ± 15 points, p < 0.001. PAQ scores relating to patient symptoms showed the largest improvement following revascularization. The smallest increases were seen in reported treatment satisfaction (all p’s < 0.001. As expected the ABI of treated limbs showed significant improvement post-revascularization (p < 0.001. ABI after revascularization correlated with patient-reported changes in the physical function and QoL domains of the PAQ. Twenty-two percent of PAD patients were identified as having a poor response to revascularization (increase in ABI < 0.15. Interestingly, poor responders reported improvement in symptoms on the PAQ, although this was less marked than in patients with an increase in ABI > 0.15 following revascularization. In conclusion, data from the current study suggest a significant correlation between improvement in patient-reported outcomes assessed by PAQ and ABI in symptomatic PAD patients undergoing peripheral revascularization.

  17. Cancer mutation screening: Comparison of high-resolution melt analysis between two platforms.

    Ebili, Henry O; Ilyas, Mohammad


    High-resolution melt analysis (HRMA) is a cheap and reliable post-polymerase chain reaction (PCR) cancer mutation screening technique, which is fast gaining clinical relevance. The HRMA capabilities of the LightScanner (Idaho Technology) have been severally studied. However, the ABI 7500 HRM has not been tested against the purpose-built HRM instrument such as the LightScanner. DNA from formalin-fixed, paraffin-embedded gastric cancer, colorectal cancer, and normal tissue as well as from colorectal cancer cell lines were amplified at exons 2, 3, and 4 of KRAS, and at exons 11 and 15 of BRAF in the ABI 7500 fast real-time PCR machine and subjected to melting both on the ABI and on the LightScanner. HRMA data were analysed with the ABI HRM software v2.0.1 and the LightScanner Call-IT 2.5. We tested the ABI 7500 HRM for internal precision, accuracy, sensitivity, and specificity at mutation screening relative to the LightScanner, using crude percentage concordance, kappa statistics, and the area under the receiver operator characteristics (AUROC) curve on SPSS version 19. The results show that the ABI 7500 HRMA has a high internal precision, and excellent concordance, sensitivity, and specificity at mutation screening compared with the LightScanner. However, in contrast to the LightScanner HRM software analysis, the ABI HRM software v.2.0.1, cannot distinguish real from certain pseudovariations in PCR amplicons that are sometimes brought about by the artefacts of the melting process. In conclusion, the ABI HRM has a comparable performance level with the LightScanner, although in certain respects mentioned previously, the LightScanner has an edge over the ABI.

  18. To screen or not to screen for peripheral arterial disease in subjects aged 80 and over in primary health care: a cross-sectional analysis from the BELFRAIL study

    Degryse Jan


    Full Text Available Abstract Background Peripheral arterial disease (PAD is common in older people. An ankle-brachial index (ABI Methods A cross-sectional study embedded within the BELFRAIL study. A general practitioner (GP centre, located in Hoeilaart, Belgium, recruited 239 patients aged 80 or older. Only three criteria for exclusion were used: urgent medical need, palliative situation and known serious dementia. The GP recorded the medical history and performed a clinical examination. The clinical research assistant performed an extensive examination including Mini-Mental State Examination (MMSE, Geriatric Depression Scale (GDS-15, Activities of Daily Living (ADL, Tinetti test and the LASA Physical Activity Questionnaire (LAPAQ. ABI was measured using an automatic oscillometric appliance. Results In 40% of patients, a reduced ABI was found. Cardiovascular risk factors were unable to identify patients with low ABI. A negative correlation was found between the number of cardiovascular morbidities and ABI. Cardiovascular morbidity had a sensitivity of 65.7% (95% CI 53.4-76.7 and a specificity of 48.6% (95% CI 38.7-58.5. Palpation of the peripheral arteries showed the highest negative predictive value (77.7% (95% CI 71.8-82.9. The LAPAQ score was significantly lower in the group with reduced ABI. Conclusion The prevalence of PAD is very high in patients aged 80 and over in general practice. The clinical examination, cardiovascular risk factors and the presence of cardiovascular morbidity were not able to identify patients with a low ABI. A screening strategy for PAD by determining ABI could be considered if effective interventions for those aged 80 and over with a low ABI become available through future research.

  19. Overview of Obstacles in the Implementation of the Argumentation Based Science Inquiry Approach and Pedagogical Suggestions



    Full Text Available The aim of this study is to investigate the possible problems confronted in implementing Argumentation Based Science Inquiry (ABI approach. In addition, the ways that teacher used to deal with those problems were further investigated in this study. For this purpose, this study utilized a case study methodology. The participant was a teacher at a primary school located in the eastern part of Turkey. The teacher took part in a project1 related to ABI. Data were collected through classroom videotape recordings and semistructured interviews. The results indicated problems in grasping the ABI by the teacher, questioning (teacher and student questioning, classroom interaction, classroom management, and accessing resources and equipment.

  20. A preliminary psychometric evaluation of the interpersonal communication competence scale for aquired brain injury

    Hald, Søren Vester; Baker, Felicity A.; Ridder, Hanne Mette Ochsner


    Primary objective: To evaluate the psychometric properties of two adapted versions of the interpersonal communication competence scale (ICCS) that were applied to people with acquired brain injury (ABI). Construct validity was tested for both new scales and a factor extraction was performed....... Participants with medium-to-severe ABI self-rated their interpersonal communication skills using the modified ICCS. Cronbach Alpha test was performed on both scales followed by a correlation analysis. Results: Seventeen participants with medium-to-severe ABI and staff and relatives (n¼37) were involved...... of the proxy-rating revealed six meaningful sub-groups of interpersonal communication competencies....