WorldWideScience

Sample records for aaa cv grande

  1. Efect of intercellular extracts from banana inoculated leaves with Mycosphaerella fijiensis Morelet, on chloroplast electronic transport of Grande naine (AAA cv.

    Directory of Open Access Journals (Sweden)

    Michel Leiva-Mora

    2003-01-01

    Full Text Available Some foliar pathogens colonize intercellular spaces of damage tissues during infection process, mediated by toxins production and diffusion to kill adjacent healthy cells. Due to the absence of reliable bioassays, the physiologic effects of several phytotoxins are still ignored on cellular membranous systems of the affected cells. In the present work it was extracted the intercellular content from not inoculated and inoculated banana leaves with different Mycosphaerella fijiensis strains. Their effects on chloroplasts of Grande naine cv were evaluated by the absorbance evolution (595 nm of Hill reactive (DCPIP, mixture with 810 ì l of chloroplasts suspension and 99 ì l of the intercellular contents. The electronic exchange on chloroplasts suspension was inhibited by intercellular contents of inoculated leaves. The intercellular contents from leaves inoculated with I1 (high virulence strain had a major inhibiter effect respect to leaves inoculates with G1 strain (low virulence, showing a correspondence between the inhibiter effect of intercellular contents and the affection levels of affected tissues. The procedures used in this work will let to make studies concerned with Mycosphaerella fijiensis-Musa spp interactions and the future breeding programs. Key words: banana breeding, black Sigatoka, host pathogen interaction, physiological bioassays

  2. BACTERIAL CONTAMINATION CONTROL IN BANANA EXPLANTS (Musa AAA cv. CAIPIRA) CONTROLE DE BACTÉRIAS CONTAMINANTES EM EXPLANTES DE BANANEIRA (Musa AAA cv. CAIPIRA)

    OpenAIRE

    Juliana Domingues Lima; Wilson da Silva Moraes

    2007-01-01

    Esse trabalho teve por objetivo testar métodos de controle de contaminação bacteriana no processo de multiplicação in vitro de bananeira (Musa AAA cv. Caipira), utilizando-se hipoclorito de sódio (NaOCl), antibiótico rifampicina e suas combinações. Não houve oxidação excessiva dos explantes após a imersão em NaOCl ou rifampicina. O melhor tratamento para explantes recém isolados foi imersão em NaOCl a 1% (v/v), dura...

  3. Early evaluation and on field conditions of resistance to Mycosphaerella fijiensis Morelet of plants from Grande naine (AAA cultivar, obtained through out tissue culture and mutations induction

    Directory of Open Access Journals (Sweden)

    Lourdes R. García

    2003-04-01

    Full Text Available The present work was carried out in the Plants Biotechnology Institute of the Central University of Las Villas. The plant material from the cv. Grande Naine (AAA was treated with physical mutagenic agents(gamma radiation 60Co source to induce genetic variability. The behaviour of the population to the black Sigatoka was evaluated. A somaclone was selected by its disease resistance and was in vitro multiplied and the plants were acclimatized to evaluate its behaviour facing the disease on greenhouse conditions and in a second cycle of multiplication in the field. The results showed that in the majority of the plants were not found differences respect cv Grande Naine, just one presented similar reaction to cv. ‘FHIA 18’ (AAAB (partially resistant as for the variable evaluated, being obtained a frequency of 0.018% for this character. This plant was named IBP 446. After 60 days of application of the mycelial homogenized of M. fijiensis in micropropagated plants of this somaclone, differences in the respect affectation states were found at susceptible witness in greenhouse conditions. When plants of the IBP 446 were evaluated in a second cycle of multiplication differences were found with the susceptible control only at flowering, while they behaved similar at susceptible control in the crop. Key words: early detection, breeding, mutation, Black Sigatoka

  4. In vitro propagation and assessment of the genetic fidelity of Musa acuminata (AAA) cv. Vaibalhla derived from immature male flowers.

    Science.gov (United States)

    Hrahsel, Lalremsiami; Basu, Adreeja; Sahoo, Lingaraj; Thangjam, Robert

    2014-02-01

    An efficient in vitro propagation method has been developed for the first time for Musa acuminata (AAA) cv. Vaibalhla, an economically important banana cultivar of Mizoram, India. Immature male flowers were used as explants. Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) were used for the regeneration process. Out of different PGR combinations, MS medium supplemented with 2 mg L(-1) 6-benzylaminopurine (BAP) + 0.5 mg L(-1) α-naphthalene acetic acid (NAA) was optimal for production of white bud-like structures (WBLS). On this medium, explants produced the highest number of buds per explant (4.30). The highest percentage (77.77) and number (3.51) of shoot formation from each explants was observed in MS medium supplemented with 2 mg L(-1) kinetin + 0.5 mg L(-1) NAA. While MS medium supplemented with a combination of 2 mg L(-1) BAP + 0.5 mg L(-1) NAA showed the maximum shoot length (14.44 cm). Rooting efficiency of the shoots was highest in the MS basal medium without any PGRs. The plantlets were hardened successfully in the greenhouse with 96% survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro regenerated plantlets of M. acuminata (AAA) cv. Vaibalhla. Eight RAPD and 8 ISSR primers were successfully used for the analysis from the 40 RAPD and 30 ISSR primers screened initially. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. The present standardised protocol will find application in mass production, conservation and genetic transformation studies of this commercially important banana.

  5. Agrobacterium tumefaciens-mediated genetic transformation of embryogenesis cell suspensions of banana cultivar Grande naine (AAA

    Directory of Open Access Journals (Sweden)

    Idalmis Bermúdez-Caraballoso

    2004-01-01

    Full Text Available The black Sigatoka (Mycosphaerella fijiensis Morelet has become in the last years, the most destructive disease that affects the production of banana and plantains world-wide. The present work was made with the objective to obtain transgenic plants of banana cultivar Grand naine (AAA resistant to this disease with the use of genetic transformation. Embryogenenic cell suspensions obtained from somatic embryos formed from immature male flowers, were used for the transformation by Agrobacterium tumefaciens. The bacterial strain EHA-105 was used with the binary plasmids pHCA-58, pHCG-59 and pHGA-91, which contain different combinations of genes that encode for the antifungal chitinase, glucanase enzymes and the AP-24 osmotin. The commercial herbicide BASTA® was used as selective agent. One hundred ten putative transformed lines of the three constructions were obtained, after three selection months in the culture medium. The transgenic events were verified by means of Polymerase Chain Reaction analysis. Key words: AP-24, chitinase, glucanase, Musa, Mycosphaerella fijiensis

  6. Ultrastructural changes and the distribution of arabinogalactan proteins during somatic embryogenesis of banana (Musa spp. AAA cv. 'Yueyoukang 1').

    Science.gov (United States)

    Pan, Xiao; Yang, Xiao; Lin, Guimei; Zou, Ru; Chen, Houbin; Samaj, Jozef; Xu, Chunxiang

    2011-08-01

    A better understanding of somatic embryogenesis in banana (Musa spp.) may provide a practical way to improve regeneration of banana plants. In this study, we applied scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to visualize the ultrastructural changes during somatic embryogenesis of banana (Musa AAA cv. 'Yueyoukang 1'). We also used histological and immunohistochemical techniques with 16 monoclonal antibodies to study the spatial distribution and cellular/subcellular localization of different arabinogalactan protein (AGP) components of the cell wall during somatic embryogenesis. Histological study with periodic acid-Schiff staining documented diverse embryogenic stages from embryogenic cells (ECs) to the late embryos. SEM revealed a mesh-like structure on the surface of proembryos which represented an early structural marker of somatic embryogenesis. TEM showed that ECs were rich in juvenile mitochondria, endoplasmic reticulum and Golgi stacks. Cells in proembryos and early globular embryos resembled ECs, but they were more vacuolated, showed more regular nuclei and slightly more developed organelles. Immunocytochemical study revealed that the signal of most AGP epitopes was stronger in starch-rich cells when compared with typical ECs. The main AGP component in the extracellular matrix surface network of banana proembryos was the MAC204 epitope. Later, AGP immunolabelling patterns varied with the developmental stages of the embryos. These results about developmental regulation of AGP epitopes along with developmental changes in the ultrastructure of cells are providing new insights into the somatic embryogenesis of banana. Copyright © Physiologia Plantarum 2011.

  7. In vitro multiplication of banana (Musa sp.) cv. Grand Naine | Ahmed ...

    African Journals Online (AJOL)

    A micropropagation method is described for banana (Musa Spp.) Cv. Grand Naine. Suckers were surface sterilized with HgCl2 (0.1%) for 6 min which gave minimum contamination with maximum culture establishment. Of various treatment combinations, Murashige and Skoog (MS) medium + BAP 4.00 mg/l with IAA 2.00 ...

  8. Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain.

    Science.gov (United States)

    Vishnevetsky, Jane; White, Thomas L; Palmateer, Aaron J; Flaishman, Moshe; Cohen, Yuval; Elad, Yigal; Velcheva, Margarita; Hanania, Uri; Sahar, Nachman; Dgani, Oded; Perl, Avihai

    2011-02-01

    The most devastating disease currently threatening to destroy the banana industry worldwide is undoubtedly Sigatoka Leaf spot disease caused by Mycosphaerella fijiensis. In this study, we developed a transformation system for banana and expressed the endochitinase gene ThEn-42 from Trichoderma harzianum together with the grape stilbene synthase (StSy) gene in transgenic banana plants under the control of the 35S promoter and the inducible PR-10 promoter, respectively. The superoxide dismutase gene Cu,Zn-SOD from tomato, under control of the ubiquitin promoter, was added to this cassette to improve scavenging of free radicals generated during fungal attack. A 4-year field trial demonstrated several transgenic banana lines with improved tolerance to Sigatoka. As the genes conferring Sigatoka tolerance may have a wide range of anti-fungal activities we also inoculated the regenerated banana plants with Botrytis cinerea. The best transgenic lines exhibiting Sigatoka tolerance were also found to have tolerance to B. cinerea in laboratory assays.

  9. Mineral fertilizer response and nutrient use efficiencies of East African highland banana (Musa spp., AAA-EAHB, cv. Kisansa)

    NARCIS (Netherlands)

    Nyombi, K.; Asten, van P.J.A.; Corbeels, M.; Taulya, G.; Leffelaar, P.A.; Giller, K.E.

    2010-01-01

    Poor yields of East African highland bananas (Musa spp., AAA-EAHB) on smallholder farms have often been attributed to problems of poor soil fertility. We measured the effects of mineral fertilizers on crop performance at two sites over two to three crop cycles; Kawanda in central Uganda and Ntungamo

  10. AAA application in diagnosis exams in a large public hospital, RS, Brazil; Aplicacao do AAA na realizacao de exames diagnosticos em um hospital publico de grande porte

    Energy Technology Data Exchange (ETDEWEB)

    Bacelar, A.; Ferret, A.A.; Vanni, S.; Galhardi, M.P.; Lykawka, R., E-mail: abacelar@hepa.ufrgs.br, E-mail: allferret@gmail.com, E-mail: svanni@hepa.ufrgs.br, E-mail: mpgalhardi@gmail.com, E-mail: rlykawka@hepa.ufrgs.br [Hospital de Clinicas de Porto Alegre (HCPA), Porto Alegre, RS (Brazil)

    2013-10-01

    Objective: the initiative AAA - Awareness , Appropriateness and Audit , promotes consciousness ( Awareness) , fitness ( Appropriateness ) and Audit ( Audit) . This paper analyzes the application of the concept in the AAA requests and justifications examinations using ionizing radiation within a large public hospital. Materials and methods: we collected and analyzed data between the years 2011 and 2012, concerning the number of exams performed with the use of radiation and their justifications. After, we sought to raise awareness of the clinical team through training on the risks and benefits of the various modalities of the radiology department and the need to justify the use of ionizing radiation on health. After the data were collected again of test requests for verification of the effectiveness of training. Results: the mean requests that need to be appropriate to the AAA in the last quarter of 2011 was 75 % lower than the average demands of the first quarter, matched against the last two months of 2012 increased by up to four times the number requests that require improvements in relation to the excellent results obtained in July 2012. Conclusion: it is shown in this paper the need of implementing this initiative AAA continuously added to the clinical staff awareness about the risk of the use of ionizing radiation, the appropriateness of the requests of these tests , as well as the control of this process in order to optimize use of ionizing radiation on health.

  11. Activation of salicylic acid metabolism and signal transduction can enhance resistance to Fusarium wilt in banana (Musa acuminata L. AAA group, cv. Cavendish).

    Science.gov (United States)

    Wang, Zhuo; Jia, Caihong; Li, Jingyang; Huang, Suzhen; Xu, Biyu; Jin, Zhiqiang

    2015-01-01

    Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubens (Foc) is the most serious disease that attacks banana plants. Salicylic acid (SA) can play a key role in plant-microbe interactions. Our study is the first to examine the role of SA in conferring resistance to Foc TR4 in banana (Musa acuminata L. AAA group, cv. Cavendish), which is the greatest commercial importance cultivar in Musa. We used quantitative real-time reverse polymerase chain reaction (qRT-PCR) to analyze the expression profiles of 45 genes related to SA biosynthesis and downstream signaling pathways in a susceptible banana cultivar (cv. Cavendish) and a resistant banana cultivar (cv. Nongke No. 1) inoculated with Foc TR4. The expression of genes involved in SA biosynthesis and downstream signaling pathways was suppressed in a susceptible cultivar and activated in a resistant cultivar. The SA levels in each treatment arm were measured using high-performance liquid chromatography. SA levels were decreased in the susceptible cultivar and increased in the resistant cultivar. Finally, we examined the contribution of exogenous SA to Foc TR4 resistance in susceptible banana plants. The expression of genes involved in SA biosynthesis and signal transduction pathways as well as SA levels were significantly increased. The results suggest that one reason for banana susceptibility to Foc TR4 is that expression of genes involved in SA biosynthesis and SA levels are suppressed and that the induced resistance observed in banana against Foc TR4 might be a case of salicylic acid-dependent systemic acquired resistance.

  12. EFECTO DE LA MICORRIZACIÓN Y LA FERTILIZACIÓN EN LA ACUMULACIÓN DE BIOMASA EN PLANTAS DE BANANO (Musa AAA cv. Gran Enano (Musaceae MICORRHIZATION AND FERTILIZATION EFFECT ON BIOMASS ACCUMULATION IN BANANA PLANTS (Musa AAA cv. Gran Enano (Musaceae

    Directory of Open Access Journals (Sweden)

    Carmen Elena Usuga Osorio

    2008-06-01

    Full Text Available Bajo condiciones de invernadero (ubicado en el municipio de Bello - Antioquia (Colombia se evaluó el efecto independiente y combinado de los factores: tipo de inóculo de Hongos Micorriza Arbuscular (HMA, fertilización y aplicación de materia orgánica sobre el porcentaje de asociación de HMA en plantas de banano (Musa AAA cv. Gran Enano, así como en la acumulación de materia seca foliar y radical. Dentro del factor tipo de inóculo, se evaluaron inóculos nativos, de agroecosistemas bananeros y ecosistemas naturales del Urabá (Antioquia-Colombia, uno comercial y la especie Acaulospora morrowiae; con respecto a la fertilización se probó la mitad, completa y dos veces la dosis de la fertilización recomendada de acuerdo al análisis de suelo y a los requerimientos de la planta, y cada uno de estos factores con y sin la aplicación de materia orgánica; como testigos se usaron, la no aplicación del respectivo factor. Se usó como material vegetal plantas de banano micropropagadas del grupo Cavendish cv. Gran Enano (AAA. El sustrato utilizado para el crecimiento de las plantas de banano se compuso de suelo y arena en relación 70/30 v/v. El suelo se obtuvo de la granja experimental de Augura, ubicado en el municipio de Carepa en la región de Urabá. Los resultados encontrados, muestran que los factores que más incidieron en la asociación así como en la acumulación de biomasa en toda la planta son la micorrización y la adición de materia orgánica. Los resultados, también muestran un comportamiento positivo respecto al uso de inóculos nativos de agroecosistemas bananeros, con bajas aplicaciones de fertilizantes.The effects of independent an combined factors such as inoculum type, fertilization and organic matter application on the percentage of association of ‘H.M.A’ in banana plants (Musa AAA cv. ‘Gran Enano’, and on the accumulation of leaves and rrots material, were evaluated under greenhouse conditions. Natives samples

  13. Role of Ethylene in Lactuca sativa cv ;Grand Rapids' Seed Germination.

    Science.gov (United States)

    Abeles, F B

    1986-07-01

    Promotion of thermoinhibited (30 degrees C) lettuce (Lactuca sativa cv ;Grand Rapids') seed germination by ethylene is similar to the action of the gas in other hormonal systems. Ethylene was more active than propylene and ethane was inactive. An inhibitor of ethylene production, aminoethoxy-vinylglycine, reduced ethylene evolution and germination. Inhibitors of ethylene action such as, 5-methyl-7-chloro-4-ethoxycarbonylmethoxy-2,1,3-benzothiadiazole, 2,5-norbornadiene, and silver thiosulfate inhibited germination and the effect was reversed by the addition of ethylene to the gas phase. The action of ethylene appears to be due to the promotion of radial cell expansion in the embryonic hypocotyl. The action of N6-benzyladenine and fusiccocin, which also overcome thermoinhibition, appears to be due to a promotion of hypocotyl elongation. None of the germination promoters studied appeared to function by lowering the mechanical resistance of the endosperm to embryonic growth. Data presented here are consistent with the view that ethylene plays a role in lettuce seed germination under thermoinhibited and normal conditions.

  14. Identification and expression analysis of four 14-3-3 genes during fruit ripening in banana (Musa acuminata L. AAA group, cv. Brazilian).

    Science.gov (United States)

    Li, Mei-Ying; Xu, Bi-Yu; Liu, Ju-Hua; Yang, Xiao-Liang; Zhang, Jian-Bin; Jia, Cai-Hong; Ren, Li-Cheng; Jin, Zhi-Qiang

    2012-02-01

    To investigate the regulation of 14-3-3 proteins in banana (Musa acuminata L. AAA group, cv. Brazilian) fruit postharvest ripening, four cDNAs encoding 14-3-3 proteins were isolated from banana and designated as Ma-14-3-3a, Ma-14-3-3c, Ma-14-3-3e, and Ma-14-3-3i, respectively. Amino acid sequence alignment showed that the four 14-3-3 proteins shared a highly conserved core structure and variable C-terminal as well as N-terminal regions with 14-3-3 proteins from other plant species. Phylogenetic analysis revealed that the four 14-3-3 genes belong to the non-ε groups. They were differentially and specifically expressed in various tissues. Real-time RT-PCR analysis indicated that these four genes function differentially during banana fruit postharvest ripening. Three genes, Ma-14-3-3a, Ma-14-3-3c, and Ma-14-3-3e, were significantly induced by exogenous ethylene treatment. However, gene function differed in naturally ripened fruits. Ethylene could induce Ma-14-3-3c expression during postharvest ripening, but expression patterns of Ma-14-3-3a and Ma-14-3-3e suggest that these two genes appear to be involved in regulating ethylene biosynthesis during fruit ripening. No obvious relationship emerged between Ma-14-3-3i expression in naturally ripened and 1-MCP (1-methylcyclopropene)-treated fruit groups during fruit ripening. These results indicate that the 14-3-3 proteins might be involved in various regulatory processes of banana fruit ripening. Further studies will mainly focus on revealing the detailed biological mechanisms of these four 14-3-3 genes in regulating banana fruit postharvest ripening.

  15. Molecular cloning and expression of five glutathione S-transferase (GST) genes from Banana (Musa acuminata L. AAA group, cv. Cavendish).

    Science.gov (United States)

    Wang, Zhuo; Huang, Suzhen; Jia, Caihong; Liu, Juhua; Zhang, Jianbin; Xu, Biyu; Jin, Zhiqiang

    2013-09-01

    Three tau class MaGSTs responded to abiotic stress, MaGSTF1 and MaGSTL1 responded to signaling molecules, they may play an important role in the growth of banana plantlet. Glutathione S-transferases (GST) are multifunctional detoxification enzymes that participate in a variety of cellular processes, including stress responses. In this study, we report the molecular characteristics of five GST genes (MaGSTU1, MaGSTU2, MaGSTU3, MaGSTF1 and MaGSTL1) cloned from banana (Musa acuminate L. AAA group, cv. Cavendish) using a RACE-PCR-based strategy. The predicted molecular masses of these GSTs range from 23.4 to 27.7 kDa and their pIs are acidic. At the amino acid level, they share high sequence similarity with GSTs in the banana DH-Pahang (AA group) genome. Phylogenetic analysis showed that the deduced amino acid sequences of MaGSTs also have high similarity to GSTs of other plant species. Expression analysis by semi-quantitative RT-PCR revealed that these genes are differentially expressed in various tissues. In addition, their expression is regulated by various stress conditions, including exposure to signaling molecules, cold, salinity, drought and Fusarium oxysporum f specialis(f. Sp) cubense Tropical Race 4 (Foc TR4) infection. The expression of the tau class MaGSTs (MaGSTU1, MaGSTU2 and MaGSTU3) mainly responded to cold, salinity and drought while MaGSTF1 and MaGSTL1 expressions were upregulated by signaling molecules. Our findings suggest that MaGSTs play a key role in both development and abiotic stress responses.

  16. Comparison of total Hg results in sediment samples from Rio Grande reservoir determine by NAA and CV AAS

    International Nuclear Information System (INIS)

    Franklin, Robson L.

    2011-01-01

    The Rio Grande reservoir is located in the Metropolitan area of Sao Paulo and it is used for recreation purposes and as source water for drinking water production. During the last decades has been detected mercury contamination in the sediments of this reservoir, mainly in the eastern part, near the main affluent of the reservoir, in the Rio Grande da Serra and Ribeirao Pires counties. In the present study bottom sediment samples were collected in four different sites into four sampling campaigns during the period of September 2008 to January 2010. The samples were dried at room temperature, ground and passed through a 2 mm sieve. Total Hg determination in the sediment samples was carried out by two different analytical techniques: neutron activation analysis (NAA) and cold vapor atomic absorption spectrometry (CV AAS). The methodology validation, in terms of precision and accuracy, was performed by reference materials, and presented a recovery of 83 to 108%. The total Hg results obtained by both analytical techniques ranged from 3 to 71 mg kg-1 and were considered similar by statistical analysis, even though NAA technique furnishes the total concentration while CV AAS using the 3015 digestion procedure characterizes only the bioavailable Hg. These results confirm that both analytical techniques were suitable to detect the Hg concentration levels in the Rio Grande sediments studied. The Hg levels in the sediment of the Rio Grande reservoir confirm the anthropogenic origin for this element in this ecosystem. (author)

  17. Effect of hydric stress induced by PEG 6000 on water content of Musa spp. ‘Grande naine’ (AAA and ‘Pelipita’ (ABB in vitro plants

    Directory of Open Access Journals (Sweden)

    Leonardo J Moreno-Bermúdez

    2015-10-01

    Full Text Available Bananas and bananas are grown in the tropical and subtropical regions of the world, by hence they are adapted to good humidity conditions. The productivity of these crops is affected by abiotic factors such as drought, which has increased in recent years due to the effects of climate change. In vitro selection of tolerant genotypes offers advantages over field selection as it saves time, resources and permit to work with larger plant volumes. The aim of this work was to determine the effect of in vitro water stress induced with polyethylene glycol 6000 (PEG 6000 on water content (WC of Musa spp. ‘Grande naine’ (AAA and ‘Pelipita’ (ABB cultivars. In ‘Pelipita’ cultivar WC was not affected by PEG 6000 effect, but in ‘Grande naine’ this trait was significantly reduced in those plants under stress. Both, ‘Pelipita’ and ‘Grande naine’ cultivars differentially respond to water stress induced in vitro by PEG 6000 and could be used as reference in plants evaluation obtained by plant breeding for drought tolerance. Key words: banana, climate change, drought, in vitro selection

  18. MULTIPLICACIÓN DE HONGOS MICORRIZA ARBUSCULAR (H.M.A Y EFECTO DE LA MICORRIZACIÓN EN PLANTAS MICROPROPAGADAS DE BANANO (Musa AAA cv. Gran Enano (Musaceae MULTIPLICATION OF ARBUSCULAR MYCORRHIZAE FUNGI (AMF AND MYCORRHIZATION EFFECT IN MICROPROPAGATED PLANTS OF BANANA (Musa AAA cv. ‘Gran Enano’ (Musaceae

    Directory of Open Access Journals (Sweden)

    Carmen Elena Usuga Osorio

    2008-06-01

    Full Text Available Se evaluó el proceso de multiplicación de hongos que forman micorriza arbuscular (HMA, para lo cual se usaron diferentes tipos de inóculos entre ellos nativos de agroecosistemas bananeros del Urabá (Antioquia-Colombia, en sustrato sólido, con diferentes plantas hospedadoras y la infectividad y efectividad sobre plantas de banano (Musa AAA cv. Gran Enano. La colonización micorrizal promedio general de los HMA a las plantas trampa fue de 37,76 ± 21,86 %, con respecto a este porcentaje, las plantas B (Brachiaria decumbens y S (Sorgum vulgare fueron las que más favorecieron la simbiosis. Teniendo en cuenta el sustrato, el S2 (Arena 50 - suelo 50 y el S6 (Vermiculita 50-suelo 50 permitieron expresiones significativamente mayores respecto a los demás. El Sorgum vulgare y Pueraria phaseoloides y en el sustrato S1 (Arena 30 - suelo 70, se encontró un mayor número de esporas. La combinación planta-sustrato que más favoreció la asociación fue la planta trampa B en los sustratos S2 y S4 (cascarilla de arroz 50-suelo50 y la producción de esporas fueron las plantas K y S en el sustrato S1. La asociación micorrícica general en plantas de banano provenientes de cultivo de tejidos fue de 48,74 ± 30,44. No se encontraron diferencias significativas (P > 0,05 entre plantas de cero días con plantas de 30 de aclimatadas. Los inóculos que significativamente favorecieron la asociación fueron los provenientes de agroecosistemas bananeros al compararse con el inóculo comercial y el proveniente de ecosistemas naturales del Urabá. El mayor peso seco foliar y radical se encontró en plántulas de banano inoculadas con I5 (Inóculo proveniente de agroecosistema bananeros de la zona de estudio. Para las variables de crecimiento no se encontraron diferencias.The process of multiplication of arbuscular mycorrhizae fungi (AMF from indigenous banana agro-environments from Urabá (Antioquia - Colombia was evaluated, using solid substrate, with different

  19. Adesope, AAA

    African Journals Online (AJOL)

    Adesope, AAA. Vol 10, No 1 (2010) - Articles Effect of waste water on vegetable production. Abstract PDF · Vol 10, No 1 (2010) - Articles Willingness to pay for safety label on sugar and vegetable oil among households in South – Western Nigeria Abstract PDF · Vol 10, No 2 (2010) - Articles Analysis of physical and chemical ...

  20. Acibenzolar-S-Metil e silício como indutores de resistência à Sigatoka-negra em bananeira cultivar Grand Naine (AAA

    Directory of Open Access Journals (Sweden)

    Cleilson do Nascimento Uchôa

    2014-03-01

    Full Text Available Uma alternativa ao uso de defensivos agrícolas no controle da Sigatoka-negra, causada pelo agente etiológico Mycosphaerella fijiensis, é a utilização da indução de resistência, que ativa os mecanismos latentes de resistência da planta com o uso de agentes bióticos ou abióticos, como por exemplo, o Acibenzolar-S-Metil (ASM e produtos a base de silício. Objetivou-se com este trabalho estudar a indução de resistência à Sigatoka-negra por ASM e Silicato de potássio. Folhas da bananeira Grand Naine (AAA foram pulverizadas com os tratamentos: T1 - Silicato de potássio a 0,5 %; T2 - 0,2 g.L-1 de ASM + Silicato de potássio a 0,5 %; T3 - 0,5 g.L-1 de ASM + Silicato de potássio a 0,5 %; T4 - 0,2 g.L-1 de ASM; T5 - 0,5 g.L-1 de ASM; T6 - 1g.L-1 de ASM; T7 - 1g.L-1 de ASM + Silicato de potássio a 0,5 %; T8 - Testemunha com inoculação e T9 - Testemunha sem inoculação com aplicação de água destilada esterilizada. Após sete dias as plantas foram inoculadas e mantidas em câmara de crescimento. Os tratamentos T1, T5, T6 e T7 não diferiram significativamente da testemunha inoculada. Os tratamentos T2, T3 e T4 reduziram a severidade da Sigatoka-negra, apresentando os respectivos valores 7,75, 8,75 e 12,75 para área abaixo da curva do progresso da doença e foram capazes de induzir a síntese conjunta de lignina e de fenóis.

  1. Efecto de la sigatoka negra (Mycosphaerella fijiensis sobre la fotosíntesis y transpiración foliar del banano (Musa sp. AAA, cv. Valery

    Directory of Open Access Journals (Sweden)

    Martín Hidalgo

    2006-01-01

    Full Text Available Con el propósito de relacionar el ataque de la Sigatoka negra (Mycosphaerella fijiensis con las tasas fotosintética y de transpiración foliares, se sembraron rebrotes del tercer ciclo de producción de una plantación del cv. Valery en potes plásticos de 4 litros. Las plantas se mantuvieron dentro de un invernadero a humedad relativa y temperatura del 90% y 27°C, respectivamente. Al emitir 6 hojas, las 4 más jóvenes fueron inoculadas con conidios de M. fijiensis a partir de cultivos monospóricos de aislamientos silvestres. Dos riegos diarios por aspersión mantuvieron una película de agua sobre las hojas. Se aplicó una suspensión de 150000 conidios ml-1 sobre el envés de las hojas utilizando un aerógrafo, en 2 fechas con un intervalo de 30 días entre ellas, para obtener diferentes grados de severidad y estadios de la enfermedad en hojas de diferentes edades. Tanto la determinación visual del porcentaje de severidad como la del estadio de desarrollo de la enfermedad, fueron llevadas a cabo en los 6,25 cm2 del área foliar cubierta por la cubeta del medidor infrarrojo de gases, utilizado para determinar las tasas fotosintéticas y transpiratorias foliares. La tasa fotosintética neta foliar (Fn; μmoles de CO2 reducidos por m2 s-1 decayó con el incremento del porcentaje de severidad (Fn=-0,1517 x+6,845; R2=0,72 y el estadio de la enfermedad (Fn=-1,62 x+8,36; R2=0,60. El impacto del patógeno sobre la tasa transpiratoria foliar (E; mmoles de agua m-2 s-1 fue relativamente menor, como lo mostró la regresión de E contra el porcentaje de severidad (E=-0,0122 x+2,429; R2=0,11

  2. Abdominal Aortic Aneurysm (AAA)

    Science.gov (United States)

    ... Professions Site Index A-Z Abdominal Aortic Aneurysm (AAA) Abdominal aortic aneurysm (AAA) occurs when atherosclerosis or plaque buildup causes the ... weak and bulge outward like a balloon. An AAA develops slowly over time and has few noticeable ...

  3. A role for α-galactosidase in the degradation of the endosperm cell walls of lettuce seeds, cv. Grand Rapids.

    Science.gov (United States)

    Leung, D W; Bewley, J D

    1983-04-01

    Isolated endosperms of Grand Rapids lettuce (Lactuca sativa L.) seeds undergo extensive cell-wall degradation and sugars are released into the surrounding incubation medium. One sugar so released is galactose. α-Galactosidase (EC 3.2.122) is present at the same level in both dry and imbibed isolated endosperms and is responsible for the release of galactose. However, this enzyme does not act upon the native endosperm cell wall, but requires first its partial hydrolysis and the production of oligomers by the action of endo-β-mannanase (EC 3.2.1.787). Galactose is then cleaved from these oligomers, allowing their further subsequent hydrolysis by endo-β-mannanase. Thus α-galactosidase and endo-β-mannanase act cooperatively to effect the hydrolysis of the lettuce endosperm cell walls.

  4. Genome-wide analysis of transcription factors during somatic embryogenesis in banana (Musa spp.) cv. Grand Naine.

    Science.gov (United States)

    Shivani; Awasthi, Praveen; Sharma, Vikrant; Kaur, Navjot; Kaur, Navneet; Pandey, Pankaj; Tiwari, Siddharth

    2017-01-01

    Transcription factors BABY BOOM (BBM), WUSCHEL (WUS), BSD, LEAFY COTYLEDON (LEC), LEAFY COTYLEDON LIKE (LIL), VIVIPAROUS1 (VP1), CUP SHAPED COTYLEDONS (CUC), BOLITA (BOL), and AGAMOUS LIKE (AGL) play a crucial role in somatic embryogenesis. In this study, we identified eighteen genes of these nine transcription factors families from the banana genome database. All genes were analyzed for their structural features, subcellular, and chromosomal localization. Protein sequence analysis indicated the presence of characteristic conserved domains in these transcription factors. Phylogenetic analysis revealed close evolutionary relationship among most transcription factors of various monocots. The expression patterns of eighteen genes in embryogenic callus containing somatic embryos (precisely isolated by Laser Capture Microdissection), non-embryogenic callus, and cell suspension cultures of banana cultivar Grand Naine were analyzed. The application of 2, 4-dichlorophenoxyacetic acid (2, 4-D) in the callus induction medium enhanced the expression of MaBBM1, MaBBM2, MaWUS2, and MaVP1 in the embryogenic callus. It suggested 2, 4-D acts as an inducer for the expression of these genes. The higher expression of MaBBM2 and MaWUS2 in embryogenic cell suspension (ECS) as compared to non-embryogenic cells suspension (NECS), suggested that these genes may play a crucial role in banana somatic embryogenesis. MaVP1 showed higher expression in both ECS and NECS, whereas MaLEC2 expression was significantly higher in NECS. It suggests that MaLEC2 has a role in the development of non-embryogenic cells. We postulate that MaBBM2 and MaWUS2 can be served as promising molecular markers for the embryogencity in banana.

  5. AAA Foundation for Traffic Safety

    Science.gov (United States)

    ... of Top Deadly Mistakes Made by Teen Drivers -- AAA AAA: Road debris causes avoidable crashes, deaths Save the ... and 500 deaths! Foundation News Stay Tuned New AAA Foundation for Traffic Safety website coming Fall 2017 ...

  6. The influence of salinity and drought stress on sodium,potassium and proline content of solanum lycopersicum l. cv. rio grande

    International Nuclear Information System (INIS)

    Ali, S.; Rab, A.

    2017-01-01

    The influence of salinity and drought stress on sodium (Na+), potassium (K+) and proline content of Solanum lycopersicum L. (tomato) cv. Rio Grande was investigated by exposing the plants to five salinity levels i.e., 0 (control), 50, 100, 150 and 200 mM NaCl and four drought regimes i.e. 0 (Control), 2, 4 and 6 days, applied from seedling (4-5 true leaves) to the harvesting stage. The means across salinity levels showed an increase in proline content and Na+ concentration but a reduced K+ concentrations, resulting in high Na+/K+ ratios in shoot and root tissue. In contrast, drought stress decreased the Na+ and K+ content, Na+/K+ ratio but increased the proline content in both the root and shoot tissue. The interaction of salinity and drought significantly affected the sodium (Na+) and potassium (K+) contents, Na+/K+ and proline content of the shoot but K+ content and proline accumulation were not significant. The root and shoot tissue of control plants (0 mMNaCl + 0 Days drought stress) had the minimum Na+ content (2316 and 3490 mu M/g D.wt.), Na+/ K+ ratio (0.399 and 0.364) and proline content (0.72 and 1.91 mu M/g F.wt.) but the highest K+ content (6399 and 9603 mu M/g D.wt.). Whereas, the Na+ content increased with salinity, the K+ content declined. It resulted in the maximum Na+/K+ ratio of the root (1.26) and shoot (0.76) with 200 mMNaCl + 0 Days drought stress. The drought stress also increased the Na+/K+ ratio. Thus, the highest Na+/K+ ratio of root (0.78) and shoot (0.77) was recorded in plants grown under 200 mMNaCl+ 6 Days drought stress. The proline content of the root and shoot were 0.462 and 1.904 mu M/g F.wt. respectively in control plants which increased with increasing salinity and drought stress duration. Thus, the maximum proline content of root (10.61 mu M/g F.wt.) and shoot (28.05 mu M/g F.wt.) was recorded in plants exposed to 200 mMNaCl + 6 days drought stress combination. (author)

  7. Multifunctional Mitochondrial AAA Proteases.

    Science.gov (United States)

    Glynn, Steven E

    2017-01-01

    Mitochondria perform numerous functions necessary for the survival of eukaryotic cells. These activities are coordinated by a diverse complement of proteins encoded in both the nuclear and mitochondrial genomes that must be properly organized and maintained. Misregulation of mitochondrial proteostasis impairs organellar function and can result in the development of severe human diseases. ATP-driven AAA+ proteins play crucial roles in preserving mitochondrial activity by removing and remodeling protein molecules in accordance with the needs of the cell. Two mitochondrial AAA proteases, i-AAA and m-AAA, are anchored to either face of the mitochondrial inner membrane, where they engage and process an array of substrates to impact protein biogenesis, quality control, and the regulation of key metabolic pathways. The functionality of these proteases is extended through multiple substrate-dependent modes of action, including complete degradation, partial processing, or dislocation from the membrane without proteolysis. This review discusses recent advances made toward elucidating the mechanisms of substrate recognition, handling, and degradation that allow these versatile proteases to control diverse activities in this multifunctional organelle.

  8. PRODUÇÃO E DESENVOLVIMENTO DA TANGERINEIRA-'MONTENEGRINA' PROPAGADA POR ENXERTIA E ESTAQUIA, NO RIO GRANDE DO SUL MANDARIN CV. 'MONTENEGRINA' PRODUCTION AND DEVELOPMENT PROPAGATED BY CUTTINGS AND GRAFTING IN RIO GRANDE DO SUL STATE, BRAZIL

    Directory of Open Access Journals (Sweden)

    GILMAR SCHÄFER

    2001-12-01

    Full Text Available A tangerineira-'Montenegrina' (Citrus deliciosa Ten., devido às boas características de sabor, conservação e colheita tardia dos frutos, é muito apreciada e cultivada no Rio Grande do Sul. Os pomares comerciais são praticamente formados por mudas enxertadas sobre Poncirus trifoliata (L. Raf. Devido à escassez de informações relativas à combinação desta cultivar com outros porta-enxertos, bem como sua propagação por estaquia, esta pesquisa objetivou estudar o comportamento inicial de plantas enxertadas sobre citrange 'Troyer' (Citrus sinensis (L. Osb. x P. trifoliata, citrumelo 'Swingle' (C. paradisi Macf. x P. trifoliata e P. trifoliata e de plantas propagadas por estaquia. O experimento foi instalado na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul - UFRGS, em junho de 1989, delineado em blocos casualisados, com cinco plantas úteis por parcela e quatro repetições. Os resultados compreenderam as primeiras safras ocorridas de 1993 a 1998. O citrumeleiro 'Swingle' foi o porta-enxerto que melhores resultados apresentou, aumentando a eficiência produtiva da tangerineira-'Montenegrina', cultivada na Depressão Central do Rio Grande do Sul, sendo que o Poncirus trifoliata diminuiu o desenvolvimento vegetativo das copas e induziu baixa eficiência produtiva. A propagação por estaquia não ofereceu vantagens em relação à enxertia sobre citrumeleiro 'Swingle' e citrangeiro 'Troyer'.The mandarin 'Montenegrina' (Citrus deliciosa Ten. yield from plants propagated either by grafting or cutting was evaluated at the 'Universidade Federal do Rio Grande do Sul' experimental agronomic station, southern Brazil, from 1993 to 1998. Three rootstocks were used in the grafted plants. The rootstocks studied were: 'Swingle' citrumelo [Citrus paradisi Macf. x Poncirus trifoliata (L. Raf.] 'Troyer' citrange (Citrus sinensis x P. trifoliata and trifoliate orange (P. trifoliata. The work was done using a randomized

  9. NASA Airborne Astronomy Ambassadors (AAA)

    Science.gov (United States)

    Backman, D. E.; Harman, P. K.; Clark, C.

    2016-12-01

    NASA's Airborne Astronomy Ambassadors (AAA) is a three-part professional development (PD) program for high school physics and astronomy teachers. The AAA experience consists of: (1) blended-learning professional development composed of webinars, asynchronous content learning, and a series of hands-on workshops (2) a STEM immersion experience at NASA Armstrong Flight Research Center's B703 science research aircraft facility in Palmdale, California, and (3) ongoing participation in the AAA community of practice (CoP) connecting participants with astrophysics and planetary science Subject Matter Experts (SMEs). The SETI Institute (SI) is partnering with school districts in Santa Clara and Los Angeles Counties during the AAA program's "incubation" period, calendar years 2016 through 2018. AAAs will be selected by the school districts based on criteria developed during spring 2016 focus group meetings led by the program's external evaluator, WestEd.. Teachers with 3+ years teaching experience who are assigned to teach at least 2 sections in any combination of the high school courses Physics (non-AP), Physics of the Universe (California integrated model), Astronomy, or Earth & Space Sciences are eligible. Partner districts will select at least 48 eligible applicants with SI oversight. WestEd will randomly assign selected AAAs to group A or group B. Group A will complete PD in January - June of 2017 and then participate in SOFIA science flights during fall 2017 (SOFIA Cycle 5). Group B will act as a control during the 2017-18 school year. Group B will then complete PD in January - June of 2018 and participate in SOFIA science flights in fall 2018 (Cycle 6). Under the current plan, opportunities for additional districts to seek AAA partnerships with SI will be offered in 2018 or 2019. A nominal two-week AAA curriculum component will be developed by SI for classroom delivery that will be aligned with selected California Draft Science Framework Disciplinary Core Ideas

  10. Multi-Centre Study on Cardiovascular Risk Management on Patients Undergoing AAA Surveillance.

    Science.gov (United States)

    Saratzis, A; Dattani, N; Brown, A; Shalhoub, J; Bosanquet, D; Sidloff, D; Stather, P

    2017-07-01

    The risk of cardiovascular events and death in patients with abdominal aortic aneurysms (AAA) is high. Screening has been introduced to reduce AAA related mortality; however, after AAA diagnosis, cardiovascular modification may be as important to patient outcomes as surveillance. The aim of this study was to assess cardiovascular risk reduction in patients with small AAA. Institutional approval was granted for The Vascular and Endovascular Research Network (VERN) to retrospectively collect data pertaining to cardiovascular risk reduction from four tertiary vascular units in England. Patients with small AAA (January 2013-December 2015) were included. Demographic details, postcode, current medications, and smoking status were recorded using a bespoke electronic database and analysed. In a secondary analysis VERN contacted all AAA screening units in England and Wales to assess their current protocols relating to CV protection. In total, 1053 patients were included (mean age 74 ± 9 years, all men). Of these, 745 patients (70.8%) had been prescribed an antiplatelet agent and 787 (74.7%) a statin. Overall, only 666 patients (63.2%) were prescribed both a statin and antiplatelet. Two hundred and sixty eight patients (32.1%) were current smokers and the proportion of patients who continued to smoke decreased with age. Overall, only 401 patients (48.1%) were prescribed a statin, antiplatelet, and had stopped smoking. In the secondary analysis 38 AAA screening units (84% national coverage) replied. Thirty-one units (82%) suggest changes to the patient's prescription; however, none monitor compliance with these recommendations or assess whether the general practitioner has been made aware of the AAA diagnosis or prescription advice. Many patients with small AAA are not prescribed an antiplatelet/statin, and still smoke cigarettes, and therefore remain at high risk of cardiovascular morbidity and mortality. National guidance to ensure this high risk group of patients is

  11. Africa Agribusiness Academy (AAA) Year Report 2014

    NARCIS (Netherlands)

    Nijhoff, G.H.; Vugt, van S.M.

    2015-01-01

    The Africa Agribusiness Academy (AAA) supports African SME agrifood companies in growing their business. An AAA member companies can enhance knowledge, skills and expertise, and get support in accessing finance and markets. By the end of 2014, AAA had 200 members in five countries: Tanzania, Kenya,

  12. Exhibition at the AAA library

    DEFF Research Database (Denmark)

    2013-01-01

    Sonnesgade 11 The exhibition at the AAA library presents selected work produced by students prior to the exhibition of installations in project and praxis constructing an archive at Sonnesgade 11. The exhibition at Sonnesgade 11 was the culmination of collaboration with SLETH architects and studio...

  13. EFFECT OF NUMBER OF FUNCTIONAL LEAVES AT FLOWERING ON YIELD OF BANANA GRAND NAINE (Musa AAA Simmonds EFECTO DEL NÚMERO DE HOJAS FUNCIONALES A LA FLORACIÓN SOBRE LA PRODUCCIÓN DE BANANO GRAN ENANO (Musa AAA Simmonds

    Directory of Open Access Journals (Sweden)

    Carolina Rodríguez González

    2012-06-01

    Full Text Available Abstract. The effect of the selective removal of leaves at flowering on yield, growth, maturity and quality of Grand Nain banana fruits was evaluated. The present study was conducted in three regions of Urabá (Colombia with precipitation low (experiment 1, medium (experiment 2 and high (experiment 3. The experimental design used randomized complete blocks, four treatments (6, 8, 10 and 12 leaves from the start of flowering and three replications. Every 15 days, the thickness (cm and length (cm of central fruit from the second hand were measured and two fruits were taken to record fresh weight and dry weight. At harvest, leaf number, bunch weight (kg, and the thickness (cm and length (cm of central fruit from the second hand were measured. During fruit ripening (8 and 15 days, the contents of starch and sugars in the pulp were determined. The thickness and length of the fruit, bunch weight and the box-stem ratio were not affected (PResumen. Se evaluó el efecto de la eliminación selectiva de hojas a la floración sobre la producción, crecimiento, maduración y calidad de los frutos de banano Gran Enano. El estudio se realizó en tres zonas de Urabá (Colombia, con precipitación baja (experimento 1, media (experimento 2 y alta (experimento 3. Se usó un diseño de bloques completamente al azar, cuatro tratamientos (6, 8, 10 y 12 hojas desde el inicio de floración y tres repeticiones. Cada 15 días se midieron el grosor (cm y la longitud (cm del fruto central de la segunda mano y se tomaron dos frutos más para registrar el peso fresco y el peso seco. A la cosecha se midieron el número de hojas, el peso del racimo (kg, el grosor (cm y la longitud (cm del fruto central de la segunda mano. Durante la maduración de los frutos (8 y 15 días se determinaron los contenidos de almidón y azúcares en la pulpa. El grosor y la longitud del fruto, peso del racimo y la cantidad de cajas a partir de un racimo (ratio, no fueron afectados (P<0,05 por la

  14. Boron toxicity in banana (Musa AAA) plantations of Costa Rica

    International Nuclear Information System (INIS)

    Vargas, Alfonso; Serrano, Edgardo; Arias, Fulvio; Arias M, Oscar

    2007-01-01

    A marginal, irregular and continuous necrosis was observed in the leaves of in banana plants (Musa AAA, cvs. Grande Naine and Valery), This necrosis was developed from an irregular chlorotic area, from the edge towards the internal part of the leaf blade. The central portion of the leaf kept the original green color. Soil and foliar analyses showed that symptoms were caused by high boron concentrations, probably due to excessive soil or foliage applications of the nutriment, or to the effect of very frequent applications of boron during fertigation, combined with a decrease of calcium in the leaf. (author) [es

  15. The LHC at the AAAS

    CERN Multimedia

    CERN Bulletin

    2011-01-01

    The American Association for the Advancement of Science held its annual meeting in the Walter E. Washington Convention Center in Washington D.C. last week.   Veteran science writer Tim Radford introduces LHC scientists during a media briefing at the AAAS annual meeting. Left to right: Felicitas Pauss, Tom LeCompte, Yves Schutz and Nick Hadley. As the world’s largest popular science meeting, the AAAS meeting is a major event in the calendar of science journalists.  At this year’s LHC session, CERN’s coordinator for international relations, Felicitas Pauss, opened the discussion, paving the way for Tom LeCompte of ATLAS, Joe Incandela of CMS, Yves Schutz of ALICE and Monica Pepe-Altarelli of LHCb to report on the status of the first year’s analysis from their experiments.    

  16. AAA-ATPases in Protein Degradation

    Directory of Open Access Journals (Sweden)

    Ravikiran S. Yedidi

    2017-06-01

    Full Text Available Proteolytic machineries containing multisubunit protease complexes and AAA-ATPases play a key role in protein quality control and the regulation of protein homeostasis. In these protein degradation machineries, the proteolytically active sites are formed by either threonines or serines which are buried inside interior cavities of cylinder-shaped complexes. In eukaryotic cells, the proteasome is the most prominent protease complex harboring AAA-ATPases. To degrade protein substrates, the gates of the axial entry ports of the protease need to be open. Gate opening is accomplished by AAA-ATPases, which form a hexameric ring flanking the entry ports of the protease. Protein substrates with unstructured domains can loop into the entry ports without the assistance of AAA-ATPases. However, folded proteins require the action of AAA-ATPases to unveil an unstructured terminus or domain. Cycles of ATP binding/hydrolysis fuel the unfolding of protein substrates which are gripped by loops lining up the central pore of the AAA-ATPase ring. The AAA-ATPases pull on the unfolded polypeptide chain for translocation into the proteolytic cavity of the protease. Conformational changes within the AAA-ATPase ring and the adjacent protease chamber create a peristaltic movement for substrate degradation. The review focuses on new technologies toward the understanding of the function and structure of AAA-ATPases to achieve substrate recognition, unfolding and translocation into proteasomes in yeast and mammalian cells and into proteasome-equivalent proteases in bacteria and archaea.

  17. AAA-ATPases in Protein Degradation.

    Science.gov (United States)

    Yedidi, Ravikiran S; Wendler, Petra; Enenkel, Cordula

    2017-01-01

    Proteolytic machineries containing multisubunit protease complexes and AAA-ATPases play a key role in protein quality control and the regulation of protein homeostasis. In these protein degradation machineries, the proteolytically active sites are formed by either threonines or serines which are buried inside interior cavities of cylinder-shaped complexes. In eukaryotic cells, the proteasome is the most prominent protease complex harboring AAA-ATPases. To degrade protein substrates, the gates of the axial entry ports of the protease need to be open. Gate opening is accomplished by AAA-ATPases, which form a hexameric ring flanking the entry ports of the protease. Protein substrates with unstructured domains can loop into the entry ports without the assistance of AAA-ATPases. However, folded proteins require the action of AAA-ATPases to unveil an unstructured terminus or domain. Cycles of ATP binding/hydrolysis fuel the unfolding of protein substrates which are gripped by loops lining up the central pore of the AAA-ATPase ring. The AAA-ATPases pull on the unfolded polypeptide chain for translocation into the proteolytic cavity of the protease. Conformational changes within the AAA-ATPase ring and the adjacent protease chamber create a peristaltic movement for substrate degradation. The review focuses on new technologies toward the understanding of the function and structure of AAA-ATPases to achieve substrate recognition, unfolding and translocation into proteasomes in yeast and mammalian cells and into proteasome-equivalent proteases in bacteria and archaea.

  18. Risk of abdominal aortic aneurysm (AAA) among male and female relatives of AAA patients.

    Science.gov (United States)

    van de Luijtgaarden, Koen M; Rouwet, Ellen V; Hoeks, Sanne E; Stolker, Robert J; Verhagen, Hence Jm; Majoor-Krakauer, Danielle

    2017-04-01

    Sex affects the presentation, treatment, and outcomes of abdominal aortic aneurysm (AAA). Although AAAs are less prevalent in women, at least in the general population, women with an AAA have a poorer prognosis in comparison to men. Sex differences in the genetic predisposition for aneurysm disease remain to be established. In this study we investigated the familial risk of AAA for women compared to men. All living AAA patients included in a 2004-2012 prospective database were invited to the multidisciplinary vascular/genetics outpatient clinic between 2009 and 2012 for assessment of family history using detailed questionnaires. AAA risk for male and female relatives was calculated separately and stratified by sex of the AAA patients. Families of 568 AAA patients were investigated and 22.5% of the patients had at least one affected relative. Female relatives had a 2.8-fold and male relatives had a 1.7-fold higher risk than the estimated sex-specific population risk. Relatives of female AAA patients had a higher aneurysm risk than relatives of male patients (9.0 vs 5.9%, p = 0.022), corresponding to 5.5- and 2.0-fold increases in aneurysm risk in the female and male relatives, respectively. The risk for aortic aneurysm in relatives of AAA patients is higher than expected from population risk. The excess risk is highest for the female relatives of AAA patients and for the relatives of female AAA patients. These findings endorse targeted AAA family screening for female and male relatives of all AAA patients.

  19. Utilização de filmes de polietileno de baixa densidade(PEBD para prolongar a vida pós-colheita de morangos, cv. oso grande The use of low density polyethylene packing to prolong the postharvest life of 'oso grande' strawberries

    Directory of Open Access Journals (Sweden)

    Joel Donazzolo

    2003-02-01

    Full Text Available Desenvolveu-se um experimento com o objetivo de avaliar o efeito da atmosfera modificada (AM, utilizando filmes de polietileno de baixa densidade (PEBD, sobre a manutenção da qualidade de morangos (Fragaria ananassa L., cv. Oso Grande, acondicionados em câmara frigorífica, a 0ºC, e em câmara de climatização, a 20ºC, simulando transporte/armazenamento refrigerado e o transporte/armazenamento convencional, sob altas temperaturas respectivamente. Os morangos foram acondicionados em bandejas plásticas com capacidade para 300 g, envoltas por filme de polivinil cloreto (PVC esticável de 15 µm de espessura. Os tratamentos constituíram-se do recobrimento de seis bandejas, alojadas em armações de madeira utilizadas comercialmente para morangos, com filmes de PEBD com 22 [T2] ou 45 µm [T3] de espessura. No tratamento-controle [T1], não se utilizou filme de PEBD. Antes do fechamento dos filmes de PEBD, foi retirado o ar e, injetado CO2, até atingir pressão parcial aproximada de 15 kPa. Todos os tratamentos foram expostos às temperaturas de 0 e 20ºC, e cada temperatura foi considerada um experimento independente. O delineamento experimental utilizado foi o de blocos ao acaso, com três repetições e a unidade experimental composta por seis bandejas com 17 frutas cada uma. As avaliações foram realizadas após sete dias para o experimento a 20ºC e, após 14 dias (com mais três dias em temperatura de 20ºC, para o experimento a 0ºC. Conforme os resultados, as frutas armazenadas a 20ºC e com filmes de PEBD apresentaram menor incidência de podridões, menor índice de cor vermelha e menor acidez titulável, e também maior firmeza de polpa, em comparação com o tratamento-controle (sem filme de PEBD. Porém, a pressão parcial de CO2 atingiu cerca de 30 kPa no interior de algumas bandejas envoltas com o filme de 45 µm, ocasionando sabor alcoólico às frutas. No experimento a 0ºC, não foram verificadas diferenças significativas

  20. Pathophysiology of AAA: heredity vs environment.

    Science.gov (United States)

    Björck, Martin; Wanhainen, Anders

    2013-01-01

    Abdominal aortic aneurysm (AAA) has a complex pathophysiology, in which both environmental and genetic factors play important roles, the most important being smoking. The recently reported falling prevalence rates of AAA in northern Europe and Australia/New Zeeland are largely explained by healthier smoking habits. Dietary factors and obesity, in particular abdominal obesity, are also of importance. A family history of AAA among first-degree relatives is present in approximately 13% of incident cases. The probability that a monozygotic twin of a person with an AAA has the disease is 24%, 71 times higher than that for a monozygotic twin of a person without AAA. Approximately 1000 SNPs in 100 candidate genes have been studied, and three genome-wide association studies were published, identifying different diverse weak associations. An example of interaction between environmental and genetic factors is the effect of cholesterol, where genetic and dietary factors affect levels of both HDL and LDL. True epigenetic studies have not yet been published. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. CTA Contribution by Evaluation of Treatment AAA

    International Nuclear Information System (INIS)

    Mikulas, J.; Majercik, M.; Klepanec, A.; Balazs, T.; Bazik, R.; Vulev, I.

    2010-01-01

    Over the past decade, the EVAR (Endovascular aortic aneurysm repair) became an accepted treatment of abdominal aortic aneurysms (AAA) as compared with the radical open surgical treatment. Due to the latest generation of stents, 60% of infra renal AAA can be treated by the endovascular method or even percutaneously, without any surgical procedure, and only under the local anesthesia. We retrospectively evaluated the findings of patients following elective EVAR who underwent CTA examination immediately after the procedure and three months after the procedure, for the period from June 2009 to February 2010 (9 months).

  2. LMIP/AAA: Local Authentication, Authorization and Accounting (AAA) Protocol for Mobile IP

    Science.gov (United States)

    Chenait, Manel

    Mobile IP represents a simple and scalable global mobility solution. However, it inhibits various vulnerabilities to malicious attacks and, therefore, requires the integration of appropriate security services. In this paper, we discuss two authentication schemes suggested for Mobile IP: standard authentication and Mobile IP/AAA authentication. In order to provide Mobile IP roaming services including identity verication, we propose an improvement to Mobile/AAA authentication scheme by applying a local politic key management in each domain, hence we reduce hando latency by avoiding the involvement of AAA infrastructure during mobile node roaming.

  3. The influence of wall stress on AAA growth and biomarkers

    NARCIS (Netherlands)

    Speelman, L.; Hellenthal, F.A.M.V.I.; Pulinx, B.; Bosboom, E.M.H.; Breeuwer, M.; Sambeek, M.R.; Vosse, van de F.N.; Jacobs, M.J.H.M.; Wodzig, W.K.W.H.; Schurink, G.W.H.

    2010-01-01

    Objectives This study investigated the relation between abdominal aortic aneurysm (AAA) wall stress, AAA growth rate and biomarker concentrations. With increasing wall stress, more damage may be caused to the AAA wall, possibly leading to progression of the aneurysm and reflection in up- or

  4. 26 CFR 1.1368-2 - Accumulated adjustments account (AAA).

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 11 2010-04-01 2010-04-01 true Accumulated adjustments account (AAA). 1.1368-2... adjustments account (AAA). (a) Accumulated adjustments account—(1) In general. The accumulated adjustments account is an account of the S corporation and is not apportioned among shareholders. The AAA is relevant...

  5. The Weekend Effect in AAA Repair.

    Science.gov (United States)

    O'Donnell, Thomas F X; Li, Chun; Swerdlow, Nicholas J; Liang, Patric; Pothof, Alexander B; Patel, Virendra I; Giles, Kristina A; Malas, Mahmoud B; Schermerhorn, Marc L

    2018-04-18

    Conflicting reports exist regarding whether patients undergoing surgery on the weekend or later in the week experience worse outcomes. We identified patients undergoing abdominal aortic aneurysm (AAA) repair in the Vascular Quality Initiative between 2009 and 2017 [n = 38,498; 30,537 endovascular aneurysm repair (EVAR) and 7961 open repair]. We utilized mixed effects logistic regression to compare adjusted rates of perioperative mortality based on the day of repair. Tuesday was the most common day for elective repair (22%), Friday for symptomatic repairs (20%), and ruptured aneurysms were evenly distributed. Patients with ruptured aneurysms experienced similar adjusted mortality whether they underwent repair during the week or on weekends. Transfers of ruptured AAA were more common over the weekend. However, patients transferred on the weekend experienced higher adjusted mortality than those transferred during the week (28% vs 21%, P = 0.02), despite the fact that during the week, transferred patients actually experienced lower adjusted mortality than patients treated at the index hospital (21% vs 31%, P AAA repair. However, patients with ruptured AAA transferred on the weekend experienced higher mortality than those transferred during the week, suggesting a need for improvement in weekend transfer processes.

  6. CV equipment responsibilities

    CERN Document Server

    Pirollet, B

    2008-01-01

    This document describes the limits of the responsibilities of the TS/CV for fire fighting equipment at the LHC. The various interfaces, providers and users of the water supply systems and clean water raising systems are described.

  7. Emgu CV essentials

    CERN Document Server

    Shi, Shin

    2013-01-01

    This book provides a practical guide to Emgu CV libraries, with sample code and examples used throughout to explain the concepts clearly. Each chapter deals with a different aspect of the Computer Vision field and the implementation of that topic in Emgu CV.If you are a C# programmer working on computer vision projects, this book is for you. You should have prior experience with C#.

  8. AAA+ Machines of Protein Destruction in Mycobacteria.

    Science.gov (United States)

    Alhuwaider, Adnan Ali H; Dougan, David A

    2017-01-01

    The bacterial cytosol is a complex mixture of macromolecules (proteins, DNA, and RNA), which collectively are responsible for an enormous array of cellular tasks. Proteins are central to most, if not all, of these tasks and as such their maintenance (commonly referred to as protein homeostasis or proteostasis) is vital for cell survival during normal and stressful conditions. The two key aspects of protein homeostasis are, (i) the correct folding and assembly of proteins (coupled with their delivery to the correct cellular location) and (ii) the timely removal of unwanted or damaged proteins from the cell, which are performed by molecular chaperones and proteases, respectively. A major class of proteins that contribute to both of these tasks are the AAA+ (ATPases associated with a variety of cellular activities) protein superfamily. Although much is known about the structure of these machines and how they function in the model Gram-negative bacterium Escherichia coli , we are only just beginning to discover the molecular details of these machines and how they function in mycobacteria. Here we review the different AAA+ machines, that contribute to proteostasis in mycobacteria. Primarily we will focus on the recent advances in the structure and function of AAA+ proteases, the substrates they recognize and the cellular pathways they control. Finally, we will discuss the recent developments related to these machines as novel drug targets.

  9. CV : [luuletused] / Mari Vallisoo

    Index Scriptorium Estoniae

    Vallisoo, Mari, 1950-2013

    2007-01-01

    Sisu: CV ; Sõnaraamat ; Tegijad ; "Kus leemekulp?..." ; "Veel üks viimnepäev mis asja teha sellega..." ; "Ülemise riiuli ääre pääl...", "Alkeemik Alberti elamus...", "Valge pulber veel enam valgega...", "Meie isa, kes sa...", "Ükskord, kui Maa...", "Kevadvesi ja -pori...", "Mõisnik Meeskurat müüs mu vaarisale..."

  10. Nutrient accumulation models in the banana (Musa AAA Simmonds cv Williams plant under nitrogen doses

    Directory of Open Access Journals (Sweden)

    Jaime Torres Bazurto

    2017-07-01

    Full Text Available This research determined the effect of four nitrogen (N doses on the nutritional behavior of (N, potassium (K, phosphorus (P, calcium (Ca and magnesium (Mg, respectively, in banana Williams, during five plant development stages and two productive cycles. The treatments were as follows: 1 absolute control, 2 0 N, 3 161 kg N ha-1, 4 321.8 kg N ha-1 and 5 483 kg N ha-1, respectively. A multivariate approach of the differences among cycles was used to adjust the models and eliminate their individual effect, with a randomized complete block design with repeated measurements over time. There were significant differences among plant development stages, with an increase in nutrient accumulation in the banana plant, there were no differences among treatments or blocks, nor in the interaction block by treatment, but the dose of 321.8 kg of N, exhibited a fructification increase in terms of N accumulation, harvest was exceeded by the dose of 483 kg of nitrogen, Ca and Mg, were the other nutrients, which showed effect at the dose of 483 kg of N but increasing only to harvest. It was concluded that high doses of nitrogen showed a trend to increase nutrient accumulation during the development of the banana plant, but especially until fructification, with the exception of Ca and Mg, which achieved the greatest accumulation in harvest.

  11. Produção convencional x integrada em pessegueiro cv. Marli na depressão central do Rio Grande do Sul Conventional production x integrated production of peaches 'Marli' in the central depression of Rio Grande do Sul state

    Directory of Open Access Journals (Sweden)

    Roseli de Mello Farias

    2003-08-01

    Full Text Available A produção integrada de frutas procura reduzir o uso de agrotóxicos, eliminar produtos considerados perigosos para a saúde humana ou prejudicial para o meio ambiente e, ao mesmo tempo, fomentar as boas práticas de manejo agrícola. Assim, os sistemas de produção convencional (PC e integrado (PI de pêssegos da cv. Marli foram comparados em relação às principais práticas de manejo da planta e do solo, controle fitossanitário, aspectos econômicos, bem como à qualidade da fruta, objetivando estabelecer o sistema de Produção Integrada de Frutas de Caroço (PIFC na Depressão Central-RS. Na área conduzida sob PI, foram utilizadas as práticas de acordo com o manejo preconizado pela Organização Internacional de Controle Biológico e no sistema de PC, aquelas de uso comum pelo produtor. A produção de pêssegos obtidas em ambos os sistemas não foi afetada. Na área de PI, houve menor número de frutas por planta quando comparada com a PC, entretanto, os pêssegos apresentaram maior tamanho e peso, não afetando a produção final. A classificação das frutas demonstrou que os pêssegos provenientes do sistema de PI são na maioria, pertencente à CAT I (diâmetro > 57 mm, enquanto os do sistema PC são de CAT II (48 a 57 mm. Houve maior incidência de Grapholita molesta e Monilinia fructicola no pomar de pêssegos provenientes do sistema de PI. O monitoramento de pragas e o manejo de doenças proporcionaram uma sensível redução na aplicação de agroquímicos. De uma forma geral é possível produzir pêssegos de melhor qualidade, mantendo a produtividade com uma redução considerável no uso de agroquímicos.The integrated production of fruits with Pit tries to reduce the use of pesticides, to eliminate products considered dangerous for human health or harmful to the environment and, at the same time, encourage good agricultural practices. Therefore the objective of the present work was to compare conventional (CP and

  12. In vitro multiplication of banana (Musa sp.) cv. Grand Naine

    African Journals Online (AJOL)

    SAM

    2014-07-02

    Jul 2, 2014 ... with IAA 2.00 mg/l resulted in maximum establishment of cultures in lesser time. MS medium + BAP. 4.00 mg/l + ... is, Musa acuminata (Malaysia) and Musa balbsiana. (India) (Georget et al., 2000). Banana plantlets produced.

  13. Advanced, Analytic, Automated (AAA) Measurement of Engagement during Learning

    Science.gov (United States)

    D'Mello, Sidney; Dieterle, Ed; Duckworth, Angela

    2017-01-01

    It is generally acknowledged that engagement plays a critical role in learning. Unfortunately, the study of engagement has been stymied by a lack of valid and efficient measures. We introduce the advanced, analytic, and automated (AAA) approach to measure engagement at fine-grained temporal resolutions. The AAA measurement approach is grounded in…

  14. Grandes remolques

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1961-07-01

    Full Text Available El empleo creciente del material pesado auxiliar en la construcción de obras de ingeniería civil ha motivado la fabricación de grandes plataformas, capaces de transportar toda clase de maquinaria auxiliar. En general, este tipo de maquinaria requiere medios de transporte, pues su circulación por carreteras es lenta, obstructiva y cara, siempre que se trate de grandes distancias, caso presente en la mayoría de ocasiones en que se exige un traslado de esta maquinaria de una a otra obra.

  15. NASA Airborne Astronomy Ambassadors (AAA) Professional Development and NASA Connections

    Science.gov (United States)

    Backman, D. E.; Clark, C.; Harman, P. K.

    2017-12-01

    NASA's Airborne Astronomy Ambassadors (AAA) program is a three-part professional development (PD) experience for high school physics, astronomy, and earth science teachers. AAA PD consists of: (1) blended learning via webinars, asynchronous content learning, and in-person workshops, (2) a STEM immersion experience at NASA Armstrong's B703 science research aircraft facility in Palmdale, California, and (3) ongoing opportunities for connection with NASA astrophysics and planetary science Subject Matter Experts (SMEs). AAA implementation in 2016-18 involves partnerships between the SETI Institute and seven school districts in northern and southern California. AAAs in the current cohort were selected by the school districts based on criteria developed by AAA program staff working with WestEd evaluation consultants. The selected teachers were then randomly assigned by WestEd to a Group A or B to support controlled testing of student learning. Group A completed their PD during January - August 2017, then participated in NASA SOFIA science flights during fall 2017. Group B will act as a control during the 2017-18 school year, then will complete their professional development and SOFIA flights during 2018. A two-week AAA electromagnetic spectrum and multi-wavelength astronomy curriculum aligned with the Science Framework for California Public Schools and Next Generation Science Standards was developed by program staff for classroom delivery. The curriculum (as well as the AAA's pre-flight PD) capitalizes on NASA content by using "science snapshot" case studies regarding astronomy research conducted by SOFIA. AAAs also interact with NASA SMEs during flight weeks and will translate that interaction into classroom content. The AAA program will make controlled measurements of student gains in standards-based learning plus changes in student attitudes towards STEM, and observe & record the AAAs' implementation of curricular changes. Funded by NASA: NNX16AC51

  16. AAAS Communicating Science Program: Reflections on Evaluation

    Science.gov (United States)

    Braha, J.

    2015-12-01

    The AAAS Center for Public Engagement (Center) with science builds capacity for scientists to engage public audiences by fostering collaboration among natural or physical scientists, communication researchers, and public engagement practitioners. The recently launched Leshner Leadership Institute empowers cohorts of mid-career scientists to lead public engagement by supporting their networks of scientists, researchers, and practitioners. The Center works closely with social scientists whose research addresses science communication and public engagement with science to ensure that the Communicating Science training program builds on empirical evidence to inform best practices. Researchers ( Besley, Dudo, & Storkdieck 2015) have helped Center staff and an external evaluator develop pan instrument that measures progress towards goals that are suggested by the researcher, including internal efficacy (increasing scientists' communication skills and confidence in their ability to engage with the public) and external efficacy (scientists' confidence in engagement methods). Evaluation results from one year of the Communicating Science program suggest that the model of training yields positive results that support scientists in the area that should lead to greater engagement. This talk will explore the model for training, which provides a context for strategic communication, as well as the practical factors, such as time, access to public engagement practitioners, and technical skill, that seems to contribute to increased willingness to engage with public audiences. The evaluation program results suggest willingness by training participants to engage directly or to take preliminary steps towards engagement. In the evaluation results, 38% of trained scientists reported time as a barrier to engagement; 35% reported concern that engagement would distract from their work as a barrier. AAAS works to improve practitioner-researcher-scientist networks to overcome such barriers.

  17. Protein quality control in organelles - AAA/FtsH story.

    Science.gov (United States)

    Janska, Hanna; Kwasniak, Malgorzata; Szczepanowska, Joanna

    2013-02-01

    This review focuses on organellar AAA/FtsH proteases, whose proteolytic and chaperone-like activity is a crucial component of the protein quality control systems of mitochondrial and chloroplast membranes. We compare the AAA/FtsH proteases from yeast, mammals and plants. The nature of the complexes formed by AAA/FtsH proteases and the current view on their involvement in degradation of non-native organellar proteins or assembly of membrane complexes are discussed. Additional functions of AAA proteases not directly connected with protein quality control found in yeast and mammals but not yet in plants are also described shortly. Following an overview of the molecular functions of the AAA/FtsH proteases we discuss physiological consequences of their inactivation in yeast, mammals and plants. The molecular basis of phenotypes associated with inactivation of the AAA/FtsH proteases is not fully understood yet, with the notable exception of those observed in m-AAA protease-deficient yeast cells, which are caused by impaired maturation of mitochondrial ribosomal protein. Finally, examples of cytosolic events affecting protein quality control in mitochondria and chloroplasts are given. This article is part of a Special Issue entitled: Protein Import and Quality Control in Mitochondria and Plastids. Copyright © 2012 Elsevier B.V. All rights reserved.

  18. Grandes cocinas

    Directory of Open Access Journals (Sweden)

    García de Castro, Emilio

    1957-11-01

    Full Text Available Se describen en este artículo una serie de aparatos para grandes cocinas, vistos por los autores durante un rápido viaje por Alemania. Aprovechando los datos obtenidos se analizan brevemente las necesidades de una gran cocina moderna, comentando los planos de las instalaciones en varios hoteles o instituciones de todo el mundo. La mayoría de la información.

  19. Initial stress and nonlinear material behavior in patient-specific AAA wall stress analysis

    NARCIS (Netherlands)

    Speelman, L.; Bosboom, E.M.H.; Schurink, G.W.H.; Buth, J.; Breeuwer, M.; Jacobs, M.J.; Vosse, van de F.N.

    2009-01-01

    Rupture risk estimation of abdominal aortic aneurysms (AAA) is currently based on the maximum diameter of the AAA. A more critical approach is based on AAA wall stress analysis. For that, in most cases, the AAA geometry is obtained from CT-data and treated as a stress free geometry. However, during

  20. Fundamental Characteristics of AAA+ Protein Family Structure and Function.

    Science.gov (United States)

    Miller, Justin M; Enemark, Eric J

    2016-01-01

    Many complex cellular events depend on multiprotein complexes known as molecular machines to efficiently couple the energy derived from adenosine triphosphate hydrolysis to the generation of mechanical force. Members of the AAA+ ATPase superfamily (ATPases Associated with various cellular Activities) are critical components of many molecular machines. AAA+ proteins are defined by conserved modules that precisely position the active site elements of two adjacent subunits to catalyze ATP hydrolysis. In many cases, AAA+ proteins form a ring structure that translocates a polymeric substrate through the central channel using specialized loops that project into the central channel. We discuss the major features of AAA+ protein structure and function with an emphasis on pivotal aspects elucidated with archaeal proteins.

  1. Advanced, Analytic, Automated (AAA) Measurement of Engagement During Learning.

    Science.gov (United States)

    D'Mello, Sidney; Dieterle, Ed; Duckworth, Angela

    2017-01-01

    It is generally acknowledged that engagement plays a critical role in learning. Unfortunately, the study of engagement has been stymied by a lack of valid and efficient measures. We introduce the advanced, analytic, and automated (AAA) approach to measure engagement at fine-grained temporal resolutions. The AAA measurement approach is grounded in embodied theories of cognition and affect, which advocate a close coupling between thought and action. It uses machine-learned computational models to automatically infer mental states associated with engagement (e.g., interest, flow) from machine-readable behavioral and physiological signals (e.g., facial expressions, eye tracking, click-stream data) and from aspects of the environmental context. We present15 case studies that illustrate the potential of the AAA approach for measuring engagement in digital learning environments. We discuss strengths and weaknesses of the AAA approach, concluding that it has significant promise to catalyze engagement research.

  2. Functional Diversity of AAA+ Protease Complexes in Bacillus subtilis

    OpenAIRE

    Elsholz, Alexander K. W.; Birk, Marlene S.; Charpentier, Emmanuelle; Turgay, K?r?ad

    2017-01-01

    Here, we review the diverse roles and functions of AAA+ protease complexes in protein homeostasis, control of stress response and cellular development pathways by regulatory and general proteolysis in the Gram-positive model organism Bacillus subtilis. We discuss in detail the intricate involvement of AAA+ protein complexes in controlling sporulation, the heat shock response and the role of adaptor proteins in these processes. The investigation of these protein complexes and their adaptor pro...

  3. Structural Elements Regulating AAA+ Protein Quality Control Machines.

    Science.gov (United States)

    Chang, Chiung-Wen; Lee, Sukyeong; Tsai, Francis T F

    2017-01-01

    Members of the ATPases Associated with various cellular Activities (AAA+) superfamily participate in essential and diverse cellular pathways in all kingdoms of life by harnessing the energy of ATP binding and hydrolysis to drive their biological functions. Although most AAA+ proteins share a ring-shaped architecture, AAA+ proteins have evolved distinct structural elements that are fine-tuned to their specific functions. A central question in the field is how ATP binding and hydrolysis are coupled to substrate translocation through the central channel of ring-forming AAA+ proteins. In this mini-review, we will discuss structural elements present in AAA+ proteins involved in protein quality control, drawing similarities to their known role in substrate interaction by AAA+ proteins involved in DNA translocation. Elements to be discussed include the pore loop-1, the Inter-Subunit Signaling (ISS) motif, and the Pre-Sensor I insert (PS-I) motif. Lastly, we will summarize our current understanding on the inter-relationship of those structural elements and propose a model how ATP binding and hydrolysis might be coupled to polypeptide translocation in protein quality control machines.

  4. A methodology for developing anisotropic AAA phantoms via additive manufacturing.

    Science.gov (United States)

    Ruiz de Galarreta, Sergio; Antón, Raúl; Cazón, Aitor; Finol, Ender A

    2017-05-24

    An Abdominal Aortic Aneurysm (AAA) is a permanent focal dilatation of the abdominal aorta at least 1.5 times its normal diameter. The criterion of maximum diameter is still used in clinical practice, although numerical studies have demonstrated the importance of biomechanical factors for rupture risk assessment. AAA phantoms could be used for experimental validation of the numerical studies and for pre-intervention testing of endovascular grafts. We have applied multi-material 3D printing technology to manufacture idealized AAA phantoms with anisotropic mechanical behavior. Different composites were fabricated and the phantom specimens were characterized by biaxial tensile tests while using a constitutive model to fit the experimental data. One composite was chosen to manufacture the phantom based on having the same mechanical properties as those reported in the literature for human AAA tissue; the strain energy and anisotropic index were compared to make this choice. The materials for the matrix and fibers of the selected composite are, respectively, the digital materials FLX9940 and FLX9960 developed by Stratasys. The fiber proportion for the composite is equal to 0.15. The differences between the composite behavior and the AAA tissue are small, with a small difference in the strain energy (0.4%) and a maximum difference of 12.4% in the peak Green strain ratio. This work represents a step forward in the application of 3D printing technology for the manufacturing of AAA phantoms with anisotropic mechanical behavior. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Toxicidad de boro en plantaciones de banano(Musa AAA en Costa Rica

    Directory of Open Access Journals (Sweden)

    Alfonso Vargas

    2007-01-01

    Full Text Available En las hojas de plantas de banano (Musa AAA, cvs. Grande Naine y Valery, se observó una necrosis marginal irregular y continua, la cual se desarrolló a partir de un área clorótica igualmente irregular, que avanzó del margen hacia el interior de la hoja. La parte central de la lámina foliar retuvo siempre su coloración verde original. Los análisis de suelo y tejido foliar mostraron que los síntomas fueron causados por altas concentraciones de boro, debido ya fuese a aplicaciones excesivas del nutrimento al suelo y al follaje, o por el efecto de aplicaciones muy frecuentes de boro vía fertirriego, combinado con una disminución de la concentración de calcio en la hoja

  6. Inhibition of early AAA formation by aortic intraluminal pentagalloyl glucose (PGG) infusion in a novel porcine AAA model

    DEFF Research Database (Denmark)

    Kloster, Brian O; Lund, Lars; Lindholt, Jes S

    2016-01-01

    to prevent or delay their expansion. In this study, we investigated whether intraluminal delivered pentagalloyl glucose (PGG) can impair the early AAA development in a porcine model. METHODS: The infrarenal aorta was exposed in thirty pigs. Twenty underwent an elastase based AAA inducing procedure and ten...... of these received an additional intraluminal PGG infusion. The final 10 were sham operated and served as controls. RESULTS: All pigs who only had an elastase infusion developed macroscopically expanding AAAs. In pigs treated with an additional PGG infusion the growth rate of the AP-diameter rapidly returned...... and histology. CONCLUSION: In our model, intraluminal delivered PGG is able to penetrate the aortic wall from the inside and impair the early AAA development by stabilizing the elastic lamellae and preserving their integrity. The principle holds a high clinical potential if it can be translated to human...

  7. Phenylalanine and tyrosine levels are rate-limiting factors in production of health promoting metabolites in Vitis vinifera cv. Gamay Red cell suspension.

    Science.gov (United States)

    Manela, Neta; Oliva, Moran; Ovadia, Rinat; Sikron-Persi, Noga; Ayenew, Biruk; Fait, Aaron; Galili, Gad; Perl, Avichai; Weiss, David; Oren-Shamir, Michal

    2015-01-01

    Environmental stresses such as high light intensity and temperature cause induction of the shikimate pathway, aromatic amino acids (AAA) pathways, and of pathways downstream from AAAs. The induction leads to production of specialized metabolites that protect the cells from oxidative damage. The regulation of the diverse AAA derived pathways is still not well understood. To gain insight on that regulation, we increased AAA production in red grape Vitis vinifera cv. Gamay Red cell suspension, without inducing external stress on the cells, and characterized the metabolic effect of this induction. Increased AAA production was achieved by expressing a feedback-insensitive bacterial form of 3-deoxy- D-arabino-heptulosonate 7-phosphate synthase enzyme (AroG (*)) of the shikimate pathway under a constitutive promoter. The presence of AroG(*) protein led to elevated levels of primary metabolites in the shikimate and AAA pathways including phenylalanine and tyrosine, and to a dramatic increase in phenylpropanoids. The AroG (*) transformed lines accumulated up to 20 and 150 fold higher levels of resveratrol and dihydroquercetin, respectively. Quercetin, formed from dihydroquercetin, and resveratrol, are health promoting metabolites that are induced due to environmental stresses. Testing the expression level of key genes along the stilbenoids, benzenoids, and phenylpropanoid pathways showed that transcription was not affected by AroG (*). This suggests that concentrations of AAAs, and of phenylalanine in particular, are rate-limiting in production of these metabolites. In contrast, increased phenylalanine production did not lead to elevated concentrations of anthocyanins, even though they are also phenylpropanoid metabolites. This suggests a control mechanism of this pathway that is independent of AAA concentration. Interestingly, total anthocyanin concentrations were slightly lower in AroG(*) cells, and the relative frequencies of the different anthocyanins changed as well.

  8. Phenylalanine and tyrosine levels are rate-limiting factors in production of health promoting metabolites in Vitis vinifera cv. Gamay Red cell suspension

    Directory of Open Access Journals (Sweden)

    Neta eManela

    2015-07-01

    Full Text Available Environmental stresses such as high light intensity and temperature cause induction of the shikimate pathway, aromatic amino acids (AAA pathways, and of pathways downstream from AAAs. The induction leads to production of specialized metabolites that protect the cells from oxidative damage. The regulation of the diverse AAA derived pathways is still not well understood. To gain insight on that regulation, we increased AAA production in red grape Vitis vinifera cv. Gamay Red cell suspension, without inducing external stress on the cells, and characterized the metabolic effect of this induction. Increased AAA production was achieved by expressing a feedback-insensitive bacterial form of 3-deoxy- D-arabino-heptulosonate 7-phosphate synthase enzyme (AroG* of the shikimate pathway under a constitutive promoter. The presence of AroG* protein led to elevated levels of primary metabolites in the shikimate and AAA pathways including phenylalanine and tyrosine, and to a dramatic increase in phenylpropanoids. The AroG* transformed lines accumulated up to 20 and 150 fold higher levels of resveratrol and dihydroquercetin, respectively. Quercetin, formed from dihydroquercetin, and resveratrol, are health promoting metabolites that are induced due to environmental stresses. Testing the expression level of key genes along the stilbenoids, benzenoids and phenylpropanoid pathways showed that transcription was not affected by AroG*. This suggests that concentrations of AAAs, and of phenylalanine in particular, are rate-limiting in production of these metabolites. In contrast, increased phenylalanine production did not lead to elevated concentrations of anthocyanins, even though they are also phenylpropanoid metabolites. This suggests a control mechanism of this pathway that is independent of AAA concentration. Interestingly, total anthocyanin concentrations were slightly lower in AroG* cells, and the relative frequencies of the different anthocyanins changed as

  9. AAA (2010) CAPD clinical practice guidelines: need for an update.

    Science.gov (United States)

    DeBonis, David A

    2017-09-01

    Review and critique of the clinical value of the AAA CAPD guidance document in light of criteria for credible and useful guidance documents, as discussed by Field and Lohr. A qualitative review of the of the AAA CAPD guidelines using a framework by Field and Lohr to assess their relative value in supporting the assessment and management of CAPD referrals. Relevant literature available through electronic search tools and published texts were used along with the AAA CAPD guidance document and the chapter by Field and Lohr. The AAA document does not meet many of the key requirements discussed by Field and Lohr. It does not reflect the current literature, fails to help clinicians understand for whom auditory processing testing and intervention would be most useful, includes contradictory suggestions which reduce clarity and appears to avoid conclusions that might cast the CAPD construct in a negative light. It also does not include input from diverse affected groups. All of these reduce the document's credibility. The AAA CAPD guidance document will need to be updated and re-conceptualised in order to provide meaningful guidance for clinicians.

  10. Assessing heterogeneity in oligomeric AAA+ machines.

    Science.gov (United States)

    Sysoeva, Tatyana A

    2017-03-01

    ATPases Associated with various cellular Activities (AAA+ ATPases) are molecular motors that use the energy of ATP binding and hydrolysis to remodel their target macromolecules. The majority of these ATPases form ring-shaped hexamers in which the active sites are located at the interfaces between neighboring subunits. Structural changes initiate in an active site and propagate to distant motor parts that interface and reshape the target macromolecules, thereby performing mechanical work. During the functioning cycle, the AAA+ motor transits through multiple distinct states. Ring architecture and placement of the catalytic sites at the intersubunit interfaces allow for a unique level of coordination among subunits of the motor. This in turn results in conformational differences among subunits and overall asymmetry of the motor ring as it functions. To date, a large amount of structural information has been gathered for different AAA+ motors, but even for the most characterized of them only a few structural states are known and the full mechanistic cycle cannot be yet reconstructed. Therefore, the first part of this work will provide a broad overview of what arrangements of AAA+ subunits have been structurally observed focusing on diversity of ATPase oligomeric ensembles and heterogeneity within the ensembles. The second part of this review will concentrate on methods that assess structural and functional heterogeneity among subunits of AAA+ motors, thus bringing us closer to understanding the mechanism of these fascinating molecular motors.

  11. AAA - University Fellowship Program for 2001: Process, Progress and Prospects (Report on the Solicitation and Award of AAA-UFP Fellowships)

    International Nuclear Information System (INIS)

    Davis, Freddie; Dixon, Cathy

    2002-01-01

    The AAA University Fellowship Program (AAA-UFP) was developed by the Amarillo National Research Center (ANRC) in fiscal year 2001 for The Department of Energy, Office of Nuclear Energy, Science, and Technology (NE), Office of Advanced Accelerator Applications (AAA). The AAA-UFP provides financial support for science and engineering students to pursue master's degrees in areas that support the AAA Program. Each fellow's thesis research must relate to the AAA program and must be approved by the Department of Energy. ANRC manages the AAA-UFP program and managed the solicitation in an open and competitive process, resulting in the award of 10 fellowships. This paper discusses the process, the response, results and recommendations for subsequent program years. (authors)

  12. The mitochondrial inner membrane AAA metalloprotease family in metazoans.

    Science.gov (United States)

    Juhola, M K; Shah, Z H; Grivell, L A; Jacobs, H T

    2000-09-15

    Three metalloproteases belonging to the AAA superfamily (Yme1p, Afg3p and Rca1p) are involved in protein turnover and respiratory chain complex assembly in the yeast inner mitochondrial membrane. Analysis of the completed genome sequences of Caenorhabditis elegans and Drosophila melanogaster indicates that this gene family typically comprises 3-4 members in metazoans. Phylogenetic analysis reveals three main branches represented, respectively, by Saccharomyces cerevisiae YME1, human SPG7 (paraplegin) and S. cerevisiae AFG3 and RCA1. mt-AAA metalloproteases are weak candidates for several previously studied Drosophila mutants. A full elucidation of the cellular and physiological roles of mt-AAA metalloproteases in metazoans will require the creation of targeted mutations.

  13. Role of AAA(+)-proteins in peroxisome biogenesis and function.

    Science.gov (United States)

    Grimm, Immanuel; Erdmann, Ralf; Girzalsky, Wolfgang

    2016-05-01

    Mutations in the PEX1 gene, which encodes a protein required for peroxisome biogenesis, are the most common cause of the Zellweger spectrum diseases. The recognition that Pex1p shares a conserved ATP-binding domain with p97 and NSF led to the discovery of the extended family of AAA+-type ATPases. So far, four AAA+-type ATPases are related to peroxisome function. Pex6p functions together with Pex1p in peroxisome biogenesis, ATAD1/Msp1p plays a role in membrane protein targeting and a member of the Lon-family of proteases is associated with peroxisomal quality control. This review summarizes the current knowledge on the AAA+-proteins involved in peroxisome biogenesis and function.

  14. Meiotic Clade AAA ATPases: Protein Polymer Disassembly Machines.

    Science.gov (United States)

    Monroe, Nicole; Hill, Christopher P

    2016-05-08

    Meiotic clade AAA ATPases (ATPases associated with diverse cellular activities), which were initially grouped on the basis of phylogenetic classification of their AAA ATPase cassette, include four relatively well characterized family members, Vps4, spastin, katanin and fidgetin. These enzymes all function to disassemble specific polymeric protein structures, with Vps4 disassembling the ESCRT-III polymers that are central to the many membrane-remodeling activities of the ESCRT (endosomal sorting complexes required for transport) pathway and spastin, katanin p60 and fidgetin affecting multiple aspects of cellular dynamics by severing microtubules. They share a common domain architecture that features an N-terminal MIT (microtubule interacting and trafficking) domain followed by a single AAA ATPase cassette. Meiotic clade AAA ATPases function as hexamers that can cycle between the active assembly and inactive monomers/dimers in a regulated process, and they appear to disassemble their polymeric substrates by translocating subunits through the central pore of their hexameric ring. Recent studies with Vps4 have shown that nucleotide-induced asymmetry is a requirement for substrate binding to the pore loops and that recruitment to the protein lattice via MIT domains also relieves autoinhibition and primes the AAA ATPase cassettes for substrate binding. The most striking, unifying feature of meiotic clade AAA ATPases may be their MIT domain, which is a module that is found in a wide variety of proteins that localize to ESCRT-III polymers. Spastin also displays an adjacent microtubule binding sequence, and the presence of both ESCRT-III and microtubule binding elements may underlie the recent findings that the ESCRT-III disassembly function of Vps4 and the microtubule-severing function of spastin, as well as potentially katanin and fidgetin, are highly coordinated. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. m-AAA proteases, mitochondrial calcium homeostasis and neurodegeneration.

    Science.gov (United States)

    Patron, Maria; Sprenger, Hans-Georg; Langer, Thomas

    2018-03-01

    The function of mitochondria depends on ubiquitously expressed and evolutionary conserved m-AAA proteases in the inner membrane. These ATP-dependent peptidases form hexameric complexes built up of homologous subunits. AFG3L2 subunits assemble either into homo-oligomeric isoenzymes or with SPG7 (paraplegin) subunits into hetero-oligomeric proteolytic complexes. Mutations in AFG3L2 are associated with dominant spinocerebellar ataxia (SCA28) characterized by the loss of Purkinje cells, whereas mutations in SPG7 cause a recessive form of hereditary spastic paraplegia (HSP7) with motor neurons of the cortico-spinal tract being predominantly affected. Pleiotropic functions have been assigned to m-AAA proteases, which act as quality control and regulatory enzymes in mitochondria. Loss of m-AAA proteases affects mitochondrial protein synthesis and respiration and leads to mitochondrial fragmentation and deficiencies in the axonal transport of mitochondria. Moreover m-AAA proteases regulate the assembly of the mitochondrial calcium uniporter (MCU) complex. Impaired degradation of the MCU subunit EMRE in AFG3L2-deficient mitochondria results in the formation of deregulated MCU complexes, increased mitochondrial calcium uptake and increased vulnerability of neurons for calcium-induced cell death. A reduction of calcium influx into the cytosol of Purkinje cells rescues ataxia in an AFG3L2-deficient mouse model. In this review, we discuss the relationship between the m-AAA protease and mitochondrial calcium homeostasis and its relevance for neurodegeneration and describe a novel mouse model lacking MCU specifically in Purkinje cells. Our results pledge for a novel view on m-AAA proteases that integrates their pleiotropic functions in mitochondria to explain the pathogenesis of associated neurodegenerative disorders.

  16. Functional Diversity of AAA+ Protease Complexes in Bacillus subtilis.

    Science.gov (United States)

    Elsholz, Alexander K W; Birk, Marlene S; Charpentier, Emmanuelle; Turgay, Kürşad

    2017-01-01

    Here, we review the diverse roles and functions of AAA+ protease complexes in protein homeostasis, control of stress response and cellular development pathways by regulatory and general proteolysis in the Gram-positive model organism Bacillus subtilis . We discuss in detail the intricate involvement of AAA+ protein complexes in controlling sporulation, the heat shock response and the role of adaptor proteins in these processes. The investigation of these protein complexes and their adaptor proteins has revealed their relevance for Gram-positive pathogens and their potential as targets for new antibiotics.

  17. Functional Diversity of AAA+ Protease Complexes in Bacillus subtilis

    Science.gov (United States)

    Elsholz, Alexander K. W.; Birk, Marlene S.; Charpentier, Emmanuelle; Turgay, Kürşad

    2017-01-01

    Here, we review the diverse roles and functions of AAA+ protease complexes in protein homeostasis, control of stress response and cellular development pathways by regulatory and general proteolysis in the Gram-positive model organism Bacillus subtilis. We discuss in detail the intricate involvement of AAA+ protein complexes in controlling sporulation, the heat shock response and the role of adaptor proteins in these processes. The investigation of these protein complexes and their adaptor proteins has revealed their relevance for Gram-positive pathogens and their potential as targets for new antibiotics. PMID:28748186

  18. The Airborne Astronomy Ambassadors (AAA) Program and NASA Astrophysics Connections

    Science.gov (United States)

    Backman, Dana Edward; Clark, Coral; Harman, Pamela

    2018-01-01

    The NASA Airborne Astronomy Ambassadors (AAA) program is a three-part professional development (PD) experience for high school physics, astronomy, and earth science teachers. AAA PD consists of: (1) blended learning via webinars, asynchronous content delivery, and in-person workshops, (2) a STEM immersion experience at NASA Armstrong’s B703 science research aircraft facility in Palmdale, California, including interactions with NASA astrophysics & planetary science Subject Matter Experts (SMEs) during science flights on SOFIA, and (3) continuing post-flight opportunities for teacher & student connections with SMEs.

  19. Instant OpenCV starter

    CERN Document Server

    Dalal, Jayneil

    2013-01-01

    Get to grips with a new technology, understand what it is and what it can do for you, and then get to work with the most important features and tasks.A practical, quick, and hands-on guide for Python developers and hobbyists who want to get started with computer vision with OpenCV.This book is great for developers, hobbyists, and students new to computer vision who are looking to get a good grounding in how to use the OpenCV library. It's assumed that you will have some basic experience in C/C++ programming.

  20. Validation of Varian's AAA algorithm with focus on lung treatments

    International Nuclear Information System (INIS)

    Roende, Heidi S.; Hoffmann, Lone

    2009-01-01

    The objective of this study was to examine the accuracy of the Anisotropic Analytical Algorithm (AAA). A variety of different field configurations in homogeneous and in inhomogeneous media (lung geometry) was tested for the AAA algorithm. It was also tested against the present Pencil Beam Convolution (PBC) algorithm. Materials and methods. Two dimensional (2D) dose distributions were measured for a variety of different field configurations in solid water with a 2D array of ion chambers. The dose distributions of patient specific treatment plans in selected transversal slices were measured in a Thorax lung phantom with Gafchromic dosimetry films. A Farmer ion chamber was used to check point doses in the Thorax phantom. The 2D dose distributions were evaluated with a gamma criterion of 3% in dose and 3 mm distance to agreement (DTA) for the 2D array measurements and for the film measurements. Results. For AAA, all fields tested in homogeneous media fulfilled the criterion, except asymmetric fields with wedges and intensity modulated plans where deviations of 5 and 4%, respectively, were seen. Overall, the measured and calculated 2D dose distributions for AAA in the Thorax phantom showed good agreement - both for 6 and 15 MV photons. More than 80% of the points in the high dose regions met the gamma criterion, though it failed at low doses and at gradients. For the PBC algorithm only 30-70% of the points met the gamma criterion. Conclusion. The AAA algorithm has been shown to be superior to the PBC algorithm in heterogeneous media, especially for 15 MV. For most treatment plans the deviations in the lung and the mediastinum regions are below 3%. However, the algorithm may underestimate the dose to the spinal cord by up to 7%

  1. Increased levels of thioredoxin in patients with abdominal aortic aneurysms (AAAs). A potential link of oxidative stress with AAA evolution

    DEFF Research Database (Denmark)

    Martinez-Pinna, R; Lindholt, Jes S.; Blanco-Colio, L M

    2010-01-01

    Oxidative stress is a main mechanism involved in vascular pathologies. Increased thioredoxin (TRX) levels have been observed in several oxidative stress-associated cardiovascular diseases. We aim to test the potential role of TRX as a biomarker of oxidative stress in abdominal aortic aneurysm (AAA)....

  2. Inhibition of early AAA formation by aortic intraluminal pentagalloyl glucose (PGG) infusion in a novel porcine AAA model.

    Science.gov (United States)

    Kloster, Brian O; Lund, Lars; Lindholt, Jes S

    2016-05-01

    The vast majority of abdominal aortic aneurysms found in screening programs are small, and as no effective treatment exits, many will expand until surgery is indicated. Therefore, it remains intriguing to develop a safe and low cost treatment of these small aneurysms, that is able to prevent or delay their expansion. In this study, we investigated whether intraluminal delivered pentagalloyl glucose (PGG) can impair the early AAA development in a porcine model. The infrarenal aorta was exposed in thirty pigs. Twenty underwent an elastase based AAA inducing procedure and ten of these received an additional intraluminal PGG infusion. The final 10 were sham operated and served as controls. All pigs who only had an elastase infusion developed macroscopically expanding AAAs. In pigs treated with an additional PGG infusion the growth rate of the AP-diameter rapidly returned to physiological values as seen in the control group. In the elastase group, histology revealed more or less complete resolution of the elastic lamellae in the media while they were more abundant, coherent and structurally organized in the PGG group. The control group displayed normal physiological growth and histology. In our model, intraluminal delivered PGG is able to penetrate the aortic wall from the inside and impair the early AAA development by stabilizing the elastic lamellae and preserving their integrity. The principle holds a high clinical potential if it can be translated to human conditions, since it, if so, potentially could represent a new drug for stabilizing small abdominal aneurysms.

  3. A structural analysis of the AAA+ domains in Saccharomyces cerevisiae cytoplasmic dynein.

    Science.gov (United States)

    Gleave, Emma S; Schmidt, Helgo; Carter, Andrew P

    2014-06-01

    Dyneins are large protein complexes that act as microtubule based molecular motors. The dynein heavy chain contains a motor domain which is a member of the AAA+ protein family (ATPases Associated with diverse cellular Activities). Proteins of the AAA+ family show a diverse range of functionalities, but share a related core AAA+ domain, which often assembles into hexameric rings. Dynein is unusual because it has all six AAA+ domains linked together, in one long polypeptide. The dynein motor domain generates movement by coupling ATP driven conformational changes in the AAA+ ring to the swing of a motile element called the linker. Dynein binds to its microtubule track via a long antiparallel coiled-coil stalk that emanates from the AAA+ ring. Recently the first high resolution structures of the dynein motor domain were published. Here we provide a detailed structural analysis of the six AAA+ domains using our Saccharomycescerevisiae crystal structure. We describe how structural similarities in the dynein AAA+ domains suggest they share a common evolutionary origin. We analyse how the different AAA+ domains have diverged from each other. We discuss how this is related to the function of dynein as a motor protein and how the AAA+ domains of dynein compare to those of other AAA+ proteins. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

  4. Transcripts and MicroRNAs Responding to Salt Stress in Musa acuminata Colla (AAA Group cv. Berangan Roots.

    Directory of Open Access Journals (Sweden)

    Wan Sin Lee

    Full Text Available Physiological responses to stress are controlled by expression of a large number of genes, many of which are regulated by microRNAs. Since most banana cultivars are salt-sensitive, improved understanding of genetic regulation of salt induced stress responses in banana can support future crop management and improvement in the face of increasing soil salinity related to irrigation and climate change. In this study we focused on determining miRNA and their targets that respond to NaCl exposure and used transcriptome sequencing of RNA and small RNA from control and NaCl-treated banana roots to assemble a cultivar-specific reference transcriptome and identify orthologous and Musa-specific miRNA responding to salinity. We observed that, banana roots responded to salinity stress with changes in expression for a large number of genes (9.5% of 31,390 expressed unigenes and reduction in levels of many miRNA, including several novel miRNA and banana-specific miRNA-target pairs. Banana roots expressed a unique set of orthologous and Musa-specific miRNAs of which 59 respond to salt stress in a dose-dependent manner. Gene expression patterns of miRNA compared with those of their predicted mRNA targets indicated that a majority of the differentially expressed miRNAs were down-regulated in response to increased salinity, allowing increased expression of targets involved in diverse biological processes including stress signaling, stress defence, transport, cellular homeostasis, metabolism and other stress-related functions. This study may contribute to the understanding of gene regulation and abiotic stress response of roots and the high-throughput sequencing data sets generated may serve as important resources related to salt tolerance traits for functional genomic studies and genetic improvement in banana.

  5. Rubisco Activases: AAA+ Chaperones Adapted to Enzyme Repair.

    Science.gov (United States)

    Bhat, Javaid Y; Thieulin-Pardo, Gabriel; Hartl, F Ulrich; Hayer-Hartl, Manajit

    2017-01-01

    Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the key enzyme of the Calvin-Benson-Bassham cycle of photosynthesis, requires conformational repair by Rubisco activase for efficient function. Rubisco mediates the fixation of atmospheric CO 2 by catalyzing the carboxylation of the five-carbon sugar ribulose-1,5-bisphosphate (RuBP). It is a remarkably inefficient enzyme, and efforts to increase crop yields by bioengineering Rubisco remain unsuccessful. This is due in part to the complex cellular machinery required for Rubisco biogenesis and metabolic maintenance. To function, Rubisco must undergo an activation process that involves carboxylation of an active site lysine by a non-substrate CO 2 molecule and binding of a Mg 2+ ion. Premature binding of the substrate RuBP results in an inactive enzyme. Moreover, Rubisco can also be inhibited by a range of sugar phosphates, some of which are "misfire" products of its multistep catalytic reaction. The release of the inhibitory sugar molecule is mediated by the AAA+ protein Rubisco activase (Rca), which couples hydrolysis of ATP to the structural remodeling of Rubisco. Rca enzymes are found in the vast majority of photosynthetic organisms, from bacteria to higher plants. They share a canonical AAA+ domain architecture and form six-membered ring complexes but are diverse in sequence and mechanism, suggesting their convergent evolution. In this review, we discuss recent advances in understanding the structure and function of this important group of client-specific AAA+ proteins.

  6. Ensacamento de frutos de pereira cv. Housui Bagging of nashi pear cv. Housui

    Directory of Open Access Journals (Sweden)

    Ivan Dagoberto Faoro

    2004-04-01

    Full Text Available Foi avaliada a qualidade de frutos de pêra japonesa cv. Housui (Pyrus pyrifolia var. culta ensacados com diferentes tipos de sacos de papel e em duas épocas: 34 e 83 dias após a florada. O ensacamento não influenciou na firmeza, no teor de sólidos solúveis totais e no peso médio dos frutos. Sacos vermelhos não induziram aumento do pH da polpa, mas o uso de sacos duplos, sacos marrons, sacos de papel kraft marrons e sacos de pipoca brancos aumentaram significativamente o pH. Ao buscar-se maior precocidade de colheita, os melhores resultados foram obtidos com o uso de sacos pequenos de papel manteiga aos 34 dias após a floração e o uso de sacos grandes duplos ou sacos grandes marrons, 83 dias após a plena floração. O ensacamento 34 dias após a plena floração, com sacos grandes de papel duplo de cor marrom ou sacos de papel kraft marrons, ou ainda o uso de sacos pequenos parafinados transparentes de papel manteiga, aos 34 dias, seguidos pela colocação, aos 83 dias, dos dois tipos de sacos grandes citados anteriormente, resultaram em frutos de melhor qualidade externa (película de coloração homogênea e mais clara, lisa e com lenticelas pouco salientes. O uso de sacos vermelhos de papel manteiga e de sacos de pipoca brancos, com ou sem ensacamento prévio com sacos pequenos de papel manteiga parafinado, não resultaram em melhoria substancial da qualidade externa do fruto.The fruit quality of Nashi pears cv. Housui (Pyrus pyrifolia var. culta have been evaluated after bagging with different paper bags at two bagging dates: 34 and 83 days after full bloom. The bagging did not affect the fruit firmness, the total soluble solids and the average fruit weight. Red bags did not affect the pH of the fruit flesh. However the fruit bagged with double bags of brown color, brown kraft paper and white popcorn paper increased the pH. In order to harvest precociously, the use of small transparent paraffin paper bags 34 days after full bloom

  7. Functional characterization of the mammalian iAAA protease subunit, YME1L

    OpenAIRE

    Majczak, Joanna

    2008-01-01

    The iAAA protease is an ATP-dependent proteolytic complex in the mitochondrial inner membrane and belongs to the highly conserved family of AAA proteins. In the yeast Saccharomyces cerevisiae, the iAAA protease is a homo-oligomeric complex composed of Yme1p subunits which are active in the intermembrane space and mediate protein quality control. Yeast cells lacking Yme1p are characterized by pleiotropic phenotypes including a respiratory deficiency at elevated temperature and an aberrant mito...

  8. Differential expression of TRAIL and its receptors relative to calcification in AAA

    International Nuclear Information System (INIS)

    Liu, Xun; Winrow, Vivienne R.; Horrocks, Michael; Stevens, Cliff R.

    2007-01-01

    Abdominal aortic aneurysm (AAA) is commonly associated with atherosclerosis. Human AAA tissue displays cells undergoing all stages of apoptosis. Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis in tumour cells but not in normal cells. It has death receptors and decoy receptors. An inhibitor of TRAIL, osteoprotegerin (OPG), is involved in osteogenesis and vascular calcification. We investigated TRAIL and its receptors in AAA compared within normal aorta (NA). Both qualitative and quantitative analyses of calcification in AAA walls were determined using Von Kossa staining and pre-operation computer tomography (CT) scans. There was a significant difference in calcification level at different locations in the AAA wall (p < 0.05). Apoptosis was confirmed in AAA by TUNEL assay. A significant difference in TRAIL and its receptor expression was observed between normal aortae and AAA (p < 0.05). Significant differences were also observed between tissues displaying different extents of calcification for TRAIL mRNA (p < 0.05) by RT-PCR examination and OPG protein (p < 0.01) by protein blotting examination. We propose that this pattern of expression of TRAIL and its receptors may contribute to AAA formation and calcification in the AAA wall

  9. Structure and evolution of N-domains in AAA metalloproteases.

    Science.gov (United States)

    Scharfenberg, Franka; Serek-Heuberger, Justyna; Coles, Murray; Hartmann, Marcus D; Habeck, Michael; Martin, Jörg; Lupas, Andrei N; Alva, Vikram

    2015-02-27

    Metalloproteases of the AAA (ATPases associated with various cellular activities) family play a crucial role in protein quality control within the cytoplasmic membrane of bacteria and the inner membrane of eukaryotic organelles. These membrane-anchored hexameric enzymes are composed of an N-terminal domain with one or two transmembrane helices, a central AAA ATPase module, and a C-terminal Zn(2+)-dependent protease. While the latter two domains have been well studied, so far, little is known about the N-terminal regions. Here, in an extensive bioinformatic and structural analysis, we identified three major, non-homologous groups of N-domains in AAA metalloproteases. By far, the largest one is the FtsH-like group of bacteria and eukaryotic organelles. The other two groups are specific to Yme1: one found in plants, fungi, and basal metazoans and the other one found exclusively in animals. Using NMR and crystallography, we determined the subunit structure and hexameric assembly of Escherichia coli FtsH-N, exhibiting an unusual α+β fold, and the conserved part of fungal Yme1-N from Saccharomyces cerevisiae, revealing a tetratricopeptide repeat fold. Our bioinformatic analysis showed that, uniquely among these proteins, the N-domain of Yme1 from the cnidarian Hydra vulgaris contains both the tetratricopeptide repeat region seen in basal metazoans and a region of homology to the N-domains of animals. Thus, it is a modern-day representative of an intermediate in the evolution of animal Yme1 from basal eukaryotic precursors. Copyright © 2015. Published by Elsevier Ltd.

  10. Prior Radiological Investigations in 65-Year-Old Men Screened for AAA.

    Science.gov (United States)

    Meecham, Lewis; Summerour, Virginia; Hobbs, Simon; Newman, Jeremy; Wall, Michael L

    2018-05-01

    The National Health Service abdominal aortic aneurysm screening programme (NAAASP) is now fully operational. Those who have previously been formally investigated for abdominal aortic aneurysm (AAA) are excluded; however, many patients undergo radiological investigation of the abdomen for other reasons. Such practices may find incidental AAA which may be eroding the performance of the NAAASP. We investigated the rates of preinvestigation before invitation to screening in our local AAA screening programme. Electronic patient records were retrospectively reviewed for all patients called between March 2013 and February 2016 in 1 local AAA screening programme. Their records were interrogated to identify any abdominal imaging within 5 years of their invitation to screening. Two thousand six hundred thirty-eight men were invited for screening; of these, 563 (21.3%) had been "prescreened". Median time between prescreening and screening was 19 months (0-60 months). Ultrasound abdomen was the most prevalent at 248 (44.0%). Two thousand two hundred forty-three (85.0%) men attended screening, and 6 (0.27%) were excluded for known AAA. Prevalence of AAA was 1.8% (n = 41). Of these, 15 (36.6%) had prior investigation with 6 (40.0%) having AAA diagnosed. Therefore, 9 (22.0%) had potential missed AAA on "prescreening" (mean diameter 35 mm [30-45], mean time lapse between investigation and screening 21.1 months [1-49]). Incidence of missed aneurysm in the "prescreened" cohort was 1.6% (9/563). Large numbers of men invited for AAA screening have undergone preinvestigation of their abdominal aorta, with 60% of the present AAA being missed. Reliance on incidental detection of AAA would leave many patients undiagnosed in the community-at risk of future rupture. Copyright © 2018 Elsevier Inc. All rights reserved.

  11. Characterization of the modular design of the autolysin/adhesin Aaa from Staphylococcus aureus.

    Science.gov (United States)

    Hirschhausen, Nina; Schlesier, Tim; Peters, Georg; Heilmann, Christine

    2012-01-01

    Staphylococcus aureus is a frequent cause of serious and life-threatening infections, such as endocarditis, osteomyelitis, pneumonia, and sepsis. Its adherence to various host structures is crucial for the establishment of diseases. Adherence may be mediated by a variety of adhesins, among them the autolysin/adhesins Atl and Aaa. Aaa is composed of three N-terminal repeated sequences homologous to a lysin motif (LysM) that can confer cell wall attachment and a C-terminally located cysteine, histidine-dependent amidohydrolase/peptidase (CHAP) domain having bacteriolytic activity in many proteins. Here, we show by surface plasmon resonance that the LysM domain binds to fibrinogen, fibronectin, and vitronectin respresenting a novel adhesive function for this domain. Moreover, we demonstrated that the CHAP domain not only mediates the bacteriolytic activity, but also adherence to fibrinogen, fibronectin, and vitronectin, thus demonstrating for the first time an adhesive function for this domain. Adherence of an S. aureus aaa mutant and the complemented aaa mutant is slightly decreased and increased, respectively, to vitronectin, but not to fibrinogen and fibronectin, which might at least in part result from an increased expression of atl in the aaa mutant. Furthermore, an S. aureus atl mutant that showed enhanced adherence to fibrinogen, fibronectin, and endothelial cells also demonstrated increased aaa expression and production of Aaa. Thus, the redundant functions of Aaa and Atl might at least in part be interchangeable. Lastly, RT-PCR and zymographic analysis revealed that aaa is negatively regulated by the global virulence gene regulators agr and SarA. We identified novel functions for two widely distributed protein domains, LysM and CHAP, i.e. the adherence to the extracellular matrix proteins fibrinogen, fibronectin, and vitronectin. The adhesive properties of Aaa might promote S. aureus colonization of host extracellular matrix and tissue, suggesting a role for

  12. Mechanism of Enzyme Repair by the AAA+ Chaperone Rubisco Activase.

    Science.gov (United States)

    Bhat, Javaid Y; Miličić, Goran; Thieulin-Pardo, Gabriel; Bracher, Andreas; Maxwell, Andrew; Ciniawsky, Susanne; Mueller-Cajar, Oliver; Engen, John R; Hartl, F Ulrich; Wendler, Petra; Hayer-Hartl, Manajit

    2017-09-07

    How AAA+ chaperones conformationally remodel specific target proteins in an ATP-dependent manner is not well understood. Here, we investigated the mechanism of the AAA+ protein Rubisco activase (Rca) in metabolic repair of the photosynthetic enzyme Rubisco, a complex of eight large (RbcL) and eight small (RbcS) subunits containing eight catalytic sites. Rubisco is prone to inhibition by tight-binding sugar phosphates, whose removal is catalyzed by Rca. We engineered a stable Rca hexamer ring and analyzed its functional interaction with Rubisco. Hydrogen/deuterium exchange and chemical crosslinking showed that Rca structurally destabilizes elements of the Rubisco active site with remarkable selectivity. Cryo-electron microscopy revealed that Rca docks onto Rubisco over one active site at a time, positioning the C-terminal strand of RbcL, which stabilizes the catalytic center, for access to the Rca hexamer pore. The pulling force of Rca is fine-tuned to avoid global destabilization and allow for precise enzyme repair. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. Multibands tunneling in AAA-stacked trilayer graphene

    Science.gov (United States)

    Redouani, Ilham; Jellal, Ahmed; Bahaoui, Abdelhadi; Bahlouli, Hocine

    2018-04-01

    We study the electronic transport through np and npn junctions for AAA-stacked trilayer graphene. Two kinds of gates are considered where the first is a single gate and the second is a double gate. After obtaining the solutions for the energy spectrum, we use the transfer matrix method to determine the three transmission probabilities for each individual cone τ = 0 , ± 1 . We show that the quasiparticles in AAA-stacked trilayer graphene are not only chiral but also labeled by an additional cone index τ. The obtained bands are composed of three Dirac cones that depend on the chirality indexes. We show that there is perfect transmission for normal or near normal incidence, which is a manifestation of the Klein tunneling effect. We analyze also the corresponding total conductance, which is defined as the sum of the conductance channels in each individual cone. Our results are numerically discussed and compared with those obtained for ABA- and ABC-stacked trilayer graphene.

  14. APC-PCI complex levels for screening of AAA in patients with peripheral atherosclerosis.

    Science.gov (United States)

    Zarrouk, Moncef; Keshavarz, Kave; Lindblad, Bengt; Gottsäter, Anders

    2013-11-01

    To evaluate the use of activated protein C-protein C inhibitor (APC-PCI) complex levels for detection of abdominal aortic aneurysm (AAA) in patients with peripheral atherosclerotic disease (PAD). APC-PCI levels and aortic diameter evaluated in 511 PAD patients without previously known AAA followed-up concerning survival for 4.8(0.5) years. AAA was found in 13% of patients. Aortic diameter correlated (r = 0.138; p = 0.002) with APC-PCI levels which were higher (0.40[0.45] vs. 0.30[0.49] μg/l; p = 0.004) in patients with AAA. This difference persisted in multivariate analysis (p = 0.029). A threshold value of APC-PCI ≥0.15 μg/L showed a specificity of 11%, a sensitivity of 97% and a negative predictive value of 96% for an AAA diagnosis. APC-PCI levels were higher in patients with AAA, and showed high sensitivity but low specificity for the diagnosis and can therefore not be considered as a screening tool in PAD patients. An AAA prevalence of 13% in patients with PAD indicates a need for AAA screening within this population.

  15. Initial stress and nonlinear material behavior in patient-specific AAA wall stress analysis

    NARCIS (Netherlands)

    Speelman, L.; Bosboom, E.M.H.; Schurink, G.W.H.; Buth, J.; Breeuwer, M.; Jacobs, M.J.H.M.; Van De Vosse, F.N.

    2009-01-01

    An abdominal aortic aneurysm (AAA) is a local dilation in the abdominal aorta of more than 50% of the original diameter and occurs in about 1 of 20 men over 65 years of age [1]. The largest threat for an AAA is rupture. Aortic repair is considered when the risk of rupture exceeds the risk of the

  16. Biomarkers for AAA: Encouraging steps but clinical relevance still to be delivered.

    Science.gov (United States)

    Htun, Nay Min; Peter, Karlheinz

    2014-10-01

    Potential biomarkers have been investigated using proteomic studies in a variety of diseases. Some biomarkers have central roles in both diagnosis and monitoring of various disorders in clinical medicine, such as troponins, brain natriuretic peptide, and C-reactive protein. Although several biomarkers have been suggested in human abdominal aortic aneurysm (AAA), reliable markers have been lacking. In this issue, Moxon et al. [Proteomics Clin Appl. 2014, 8, 762-772] undertook a broad and systematic proteomic approach toward identification of biomarkers in a commonly used AAA mouse model (AAA created by angiotensin-II infusion). In this mouse model, apolipoprotein C1 and matrix metalloproteinase-9 were identified as novel biomarkers of stable AAA. This finding represents an important step forward, toward a clinically relevant role of biomarkers in AAA. This also encourages for further studies toward the identification of biomarkers (or their combinations) that can predict AAA progression and rupture, which would represent a major progress in AAA management and would establish an AAA biomarker as a much anticipated clinical tool. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Combination of endovascular graft exclusion and drug therapy in AAA with hypertension or hyperglycemia.

    Science.gov (United States)

    Wang, Dile; Qu, Bihui; He, Tao

    2017-08-01

    The objective of the present study was to evaluate the efficacy of combination of endovascular graft exclusion and drugs for hypertension/hyperglycemia for the treatment of abdominal aortic aneurysm (AAA). We analyzed 156 patients with AAA. Eighty-four patients were hypertensive and 72 were hyperglycemic. After endovascular graft exclusion, hypertensive patients were divided into four groups and treated with cyclopenthiazide, reserpine, propranolol, and placebo respectively. Hyperglycemic patients were divided into three groups and treated with metformin, insulin, and placebo respectively. Body temperature and peripheral blood leukocytes were measured at day 1, 2, 7, and 14 after endovascular graft exclusion. Size of AAAs, blood pressure, and blood sugar were measured again after 1 year. In hypertensive patients, the size of AAAs reduced after endovascular graft exclusion, while the combined treatments with cyclopenthiazide, reserpine, or propranolol helped to reduce blood pressure (blood pressure decrease AAA size decreased in the control group (PAAAs reduced after endovascular graft exclusion. Combined treatment with Metformin and Insulin reduced blood sugar (control, blood sugar >7.8 mmol/L (22/24), AAA size (P7.8 mmol/L (14/24), AAA size (P7.8 mmol/L (11/24), AAA size (PAAA therapy.

  18. Aortocaval fistula (ACF) in patients operated for ruptured aortic aneurysm (rAAA)

    DEFF Research Database (Denmark)

    Warning, Karina; Houlind, Kim Christian; Ravn, Hans

    Aortocaval fistula (ACF) in patients operated for Ruptured Acute Aorta Aneurysm (rAAA): A surgical challenge. Introduction Aortocaval fistula is a rare complication of abdominal aortic aneurysm (AAA). It been reported to be present in less than 1% of all AAAs. ACF arise in patients with large...... aneurysms and is typically discovered peroperatively in patients with rAAA. Open surgical treatment is associated with high mortality and morbidity. ACF is a result of spontaneously rupture of large atherosclerotic aneurysms into the inferior vena cava in 80%, 15% arise after trauma and 5% are iatrogenic....... Patients During the last 9 month, we have operated three cases with rAAA and ACF. All patients had an open, acute operation. All ACFs detected during operation. Common for all operations was that the patients had large aneurysms (>10 cm) and extensive bleeding (3-4 l) immediately when the aneurysm...

  19. INTERSTOCK EFFECT ON THE GROWTH OF MANDARIN CV BATU 55, TANGERINE CV PONTIANAK AND LIME CV NIMAS PROPAGATED BY GRAFTING

    Directory of Open Access Journals (Sweden)

    Sugiyatno A.

    2017-10-01

    Full Text Available Japansche citroen (JC rootstock is still the main choice for producing citrus seeds for its high availability and compatibility. Other rootstocks have been used in the production, yet they produce unsatisfying results. Based on the theory, there is an alternative to use rootstock as interstock in order to stimulate the growth of scion. The objective of this reseach is to understand the effect of interstock on the growth of Mandarin cv. Batu 55, Tangerine cv. Pontianak and Lime cv. Nimas. This research was conducted in the screen house of Tlekung Experimental Field of Indonesian Citrus and Subtropical Fruits Research Institute (ICSFRI in Batu-East Java, located on 950 m asl, from January to April 2016. This research was arranged in a CRD design with 4 replications and 24 interstock-scion combination treatments. The interstocks used in this study were Carizzo citrange, Citrumello, Poncirus trifoliata, Volkameriana, Rough lemon, Troyer citrange, Kanci and control (JC combined with 3 scions namely Mandarin cv. Batu 55, Tangerine cv. Pontianak and Lime cv. Nimas propagated by grafting. The result showed the percentage of successful grafting ranged between 37.5% to 100%, while the percentage of the shoot formed on grafted plants was 75%-100%. The use of Poncirus trifoliata as interstock has stimulated fastest bud break on Tangerine cv. Pontianak in just 24.75 days while the Troyer citrange has stimulated better shoot growth in Lime cv. Nimas, resulted to the highest shoot around 52.20 cm which was significantly different from other treatments. Meanwhile, Carizzo citrange has been found to have some influences to the plant diameter and the number of leave of Lime cv. Nimas but showed insignificant effect on the growth of its own diameter. The use of interstock showed that it can be used to promote the growth of scion which usually has slow growth when it is directly grafted on the rootstock.

  20. Efeito de diferentes sanificantes sobre a qualidade de morango cv. Oso Grande Effect of different sanitizing on the quality of strawberry cv. Oso Grande

    Directory of Open Access Journals (Sweden)

    Kelen Cristina dos Reis

    2008-02-01

    Full Text Available Conduziu-se o presente trabalho para estudar o efeito de diferentes sanificantes, na conservação pós-colheita de morangos, armazenados sob refrigeração (9± 1ºC e 99% UR, por um período de 12 dias. O delineamento experimental utilizado foi inteiramente casualizado, com 3 repetições, constituindo dos seguintes tratamentos: T1 Controle, imersão em água destilada, 15 min, T2 imersão em solução de dicloro s. triazinatriona sódica dihidratada (Sumaveg® a 200 ppm, por 15 min; T3 imersão em solução de peróxido de hidrogênio 10%, por 15 min; T4 imersão em solução de dicloro isocianurato de sódio (Hidrosan® a 200 ppm, por 15 min. As análises de pH, SS, AT, Cor L*a*b e microbiológicas foram realizadas a cada 3 dias. Os processos de sanificação adotados foram eficientes para manter a qualidade de morangos minimamente processados por 12 dias de armazenamento. Verificou-se, no presente trabalho, que o uso de sanificantes foi de fundamental importância para manter baixas as contagens de fungos filamentosos e leveduras dos morangos minimamente processados.The aim of this work was to study the effect of different sanitizing in the conservation postharvest of strawberry, stored in 9ºC and 99% R.H. for 12 days. The experiment was conducted in completely randomized design, with three replicates, the treatments constituted by: T1 Control, immersion in distilled water; T2 immersion in solution of dychlorine s. triazinatriona sodiumdehydrated (Sumaveg® at 200 ppm, for 15 min; T3 immersion in solution of hydrogen peroxide 10%, for 15 min; T4 immersion in solution of dychlorine isocianurate of sodium (Hidrosan® 200 ppm, for 15 min. The analyses of pH, SS (soluble solids, TA (titrable acidity, Color L*a*b and microbiological was evaluated in 0, 3, 6, 9 and 12 days. The process of sanitizing was efficient to maintain the quality of strawberry minimally processed for 12 days. The use of sanitizing was of fundamental importance to maintain low containing of microorganisms of the group mold and yeasts.

  1. Loss of Drosophila i-AAA protease, dYME1L, causes abnormal mitochondria and apoptotic degeneration.

    Science.gov (United States)

    Qi, Y; Liu, H; Daniels, M P; Zhang, G; Xu, H

    2016-02-01

    Mitochondrial AAA (ATPases Associated with diverse cellular Activities) proteases i-AAA (intermembrane space-AAA) and m-AAA (matrix-AAA) are closely related and have major roles in inner membrane protein homeostasis. Mutations of m-AAA proteases are associated with neuromuscular disorders in humans. However, the role of i-AAA in metazoans is poorly understood. We generated a deletion affecting Drosophila i-AAA, dYME1L (dYME1L(del)). Mutant flies exhibited premature aging, progressive locomotor deficiency and neurodegeneration that resemble some key features of m-AAA diseases. dYME1L(del) flies displayed elevated mitochondrial unfolded protein stress and irregular cristae. Aged dYME1L(del) flies had reduced complex I (NADH/ubiquinone oxidoreductase) activity, increased level of reactive oxygen species (ROS), severely disorganized mitochondrial membranes and increased apoptosis. Furthermore, inhibiting apoptosis by targeting dOmi (Drosophila Htra2/Omi) or DIAP1, or reducing ROS accumulation suppressed retinal degeneration. Our results suggest that i-AAA is essential for removing unfolded proteins and maintaining mitochondrial membrane architecture. Loss of i-AAA leads to the accumulation of oxidative damage and progressive deterioration of membrane integrity, which might contribute to apoptosis upon the release of proapoptotic molecules such as dOmi. Containing ROS level could be a potential strategy to manage mitochondrial AAA protease deficiency.

  2. Grand Hotel prijutil hudozhnikov

    Index Scriptorium Estoniae

    2004-01-01

    Raadioajakirjanik Lea Veelmaa lindistas "Kunstikanali" 2004. a. esimese saate Grand Hotel Viljandis. Saatekülaliseks oli maalikunstnik Andres Tolts. Toltsi kaheksa akrüülmaali on eksponeeritud hotelli fuajees ja restoranis

  3. Grand Mal Seizure

    Science.gov (United States)

    ... grand mal seizures include: A family history of seizure disorders Any injury to the brain from trauma, a ... the risk of birth defects. If you have epilepsy and plan to become pregnant, work with your ...

  4. Parallel Agent-as-a-Service (P-AaaS Based Geospatial Service in the Cloud

    Directory of Open Access Journals (Sweden)

    Xicheng Tan

    2017-04-01

    Full Text Available To optimize the efficiency of the geospatial service in the flood response decision making system, a Parallel Agent-as-a-Service (P-AaaS method is proposed and implemented in the cloud. The prototype system and comparisons demonstrate the advantages of our approach over existing methods. The P-AaaS method includes both parallel architecture and a mechanism for adjusting the computational resources—the parallel geocomputing mechanism of the P-AaaS method used to execute a geospatial service and the execution algorithm of the P-AaaS based geospatial service chain, respectively. The P-AaaS based method has the following merits: (1 it inherits the advantages of the AaaS-based method (i.e., avoiding transfer of large volumes of remote sensing data or raster terrain data, agent migration, and intelligent conversion into services to improve domain expert collaboration; (2 it optimizes the low performance and the concurrent geoprocessing capability of the AaaS-based method, which is critical for special applications (e.g., highly concurrent applications and emergency response applications; and (3 it adjusts the computing resources dynamically according to the number and the performance requirements of concurrent requests, which allows the geospatial service chain to support a large number of concurrent requests by scaling up the cloud-based clusters in use and optimizes computing resources and costs by reducing the number of virtual machines (VMs when the number of requests decreases.

  5. Assessment of the accuracy of AortaScan for detection of abdominal aortic aneurysm (AAA).

    Science.gov (United States)

    Abbas, A; Smith, A; Cecelja, M; Waltham, M

    2012-02-01

    AortaScan AMI 9700 is a portable 3D ultrasound device that automatically measures the maximum diameter of the abdominal aorta without the need for a trained sonographer. It is designed to rapidly diagnose or exclude an AAA and may have particular use in screening programs. Our objective was to determine its accuracy to detect AAA. Subjects from our AAA screening and surveillance programs were examined. The aorta was scanned using the AortaScan and computed tomography (CT). Ninety-one subjects underwent imaging (44 AAA on conventional ultrasound surveillance and 47 controls). The largest measurement obtained by AortaScan was compared against the CT-aortic measurement. The mean aortic diameter was 2.8 cm. The CT scan confirmed the diagnosis of AAA in 43 subjects. There was one false positive measurement on conventional ultrasound. AortaScan missed the diagnosis of AAA in eight subjects. There were thirteen false positive measurements. The sensitivity, specificity, positive and negative predictive values were 81%, 72%, 72% and 81% respectively. A device to detect AAA without the need for a trained operator would have potential in a community-based screening programme. The AortaScan, however, lacks adequate sensitivity and significant technical improvement is necessary before it could be considered a replacement for trained screening personnel. Copyright © 2011 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.

  6. Mitochondrial AAA proteases--towards a molecular understanding of membrane-bound proteolytic machines.

    Science.gov (United States)

    Gerdes, Florian; Tatsuta, Takashi; Langer, Thomas

    2012-01-01

    Mitochondrial AAA proteases play an important role in the maintenance of mitochondrial proteostasis. They regulate and promote biogenesis of mitochondrial proteins by acting as processing enzymes and ensuring the selective turnover of misfolded proteins. Impairment of AAA proteases causes pleiotropic defects in various organisms including neurodegeneration in humans. AAA proteases comprise ring-like hexameric complexes in the mitochondrial inner membrane and are functionally conserved from yeast to man, but variations are evident in the subunit composition of orthologous enzymes. Recent structural and biochemical studies revealed how AAA proteases degrade their substrates in an ATP dependent manner. Intersubunit coordination of the ATP hydrolysis leads to an ordered ATP hydrolysis within the AAA ring, which ensures efficient substrate dislocation from the membrane and translocation to the proteolytic chamber. In this review, we summarize recent findings on the molecular mechanisms underlying the versatile functions of mitochondrial AAA proteases and their relevance to those of the other AAA+ machines. Copyright © 2011 Elsevier B.V. All rights reserved.

  7. Wyoming CV Pilot Traveler Information Message Sample

    Data.gov (United States)

    Department of Transportation — This dataset contains a sample of the sanitized Traveler Information Messages (TIM) being generated by the Wyoming Connected Vehicle (CV) Pilot. The full set of TIMs...

  8. cv. “Sukali Ndiizi”- AAB

    African Journals Online (AJOL)

    Clara

    2013-03-27

    Mar 27, 2013 ... improve transformation and regeneration efficiency of banana (cv. “Sukali .... Vuylsteke (1989) proliferation medium in the tissue culture .... DNA repair machinery is more active during cell division ... Biology-Plant 40:31-45.

  9. Different gamma radiation doses effects of 60 Co on buds of banana plant (Nanicao-AAA) breeded in vitro

    International Nuclear Information System (INIS)

    Alves, Gilberto Dias; Colaco, Waldeciro

    1999-01-01

    Buds from banana cv. Nanicao-AAA (3 mm x 3 mm) were aseptically cultured in a modified Murashige and Skoog (MS) basal medium supplement with bezylaminepurine and sucrose, and solidified with agar. Buds placed on sterile Petri dishes were irradiated with increasing gamma rays doses (10, 20, 30, and 40 Gy). The statistical design was completely randomized with 5 doses and 30 replications. After irradiation, buds were transferred to 10 ml of the same medium into test tubes, and allowed to grow in a controlled environment (25 deg C, 16 h illumination ) for 4 weeks. There were no significant differences (Tukey, 0,05) between doses in terms of oxidation: in around 20% for all treatments. On the other hand, a statistically significant decrease in the germination of new buds with increased doses of irradiation was observed. The treatment with 40 Gy reduced in 80% the germination of new buds by the end of the evaluated period (4 weeks), resulting in a mean production of 1,5 buds. Mean production in the control was 7,6 buds. no statistically significant differences were detected between treatments with 10, 20, and 30 Gy, with a mean production of 3 buds, less than half obtained in the control. (author)

  10. Selective Intra-procedural AAA sac Embolization During EVAR Reduces the Rate of Type II Endoleak.

    Science.gov (United States)

    Mascoli, C; Freyrie, A; Gargiulo, M; Gallitto, E; Pini, R; Faggioli, G; Serra, C; De Molo, C; Stella, A

    2016-05-01

    The pre-treatment presence of at least six efferent patent vessels (EPV) from the AAA sac and/or AAA thrombus volume ratio (VR%) AAA sac embolization (Group A, 2012-2013) were retrospectively selected and compared with a control group of patients with the same p-MRF, who underwent EVAR without intra-procedural sac embolization (Group B, 2008-2010). The presence of ELIIp was evaluated by duplex ultrasound at 0 and 6 months, and by contrast enhanced ultrasound at 12 months. The association between AAA diameter, age, COPD, smoking, anticoagulant therapy, and AAA sac embolization with ELIIp was evaluated using multiple logistic regression. The primary endpoint was the effectiveness of the intra-procedural AAA sac embolization for ELIIp prevention. Secondary endpoints were AAA sac evolution and freedom from ELIIp and embolization related re-interventions at 6-12 months. Seventy patients were analyzed: 26 Group A and 44 Group B; the groups were homogeneous for clinical/morphological characteristics. In Group A the median number of coils positioned in AAA sac was 4.1 (IQR 1). There were no complications related to the embolization procedures. A significantly lower number of ELIIp was detected in Group A than in Group B (8/26 vs. 33/44, respectively, p AAA sac embolization was the only factor independently associated with freedom from ELIIp at 6 (OR 0.196, 95% CI 0.06-0.63; p = .007) and 12 months (OR 0.098, 95% CI 0.02-0.35; p AAA sac diameter shrinkage were detected between the two groups at 6-12 months (p = .42 and p = .58, respectively). Freedom from ELIIp related and embolization related re-interventions was 100% in both groups, at 6 and 12 months. Selective intra-procedural AAA sac embolization in patients with p-MRF is safe and could be an effective method to reduce ELIIp. Further studies are mandatory to support these results at long-term follow up. Copyright © 2015 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.

  11. Inflammatory cell phenotypes in AAAs; their role and potential as targets for therapy

    OpenAIRE

    Dale, Matthew A; Ruhlman, Melissa K.; Baxter, B. Timothy

    2015-01-01

    Abdominal aortic aneurysms are characterized by chronic inflammatory cell infiltration. AAA is typically an asymptomatic disease and caused approximately 15,000 deaths annually in the U.S. Previous studies have examined both human and murine aortic tissue for the presence of various inflammatory cell types. Studies show that in both human and experimental AAAs, prominent inflammatory cell infiltration, such as CD4+ T cells and macrophages, occurs in the damaged aortic wall. These cells have t...

  12. Electron cryomicroscopy structure of a membrane-anchored mitochondrial AAA protease.

    Science.gov (United States)

    Lee, Sukyeong; Augustin, Steffen; Tatsuta, Takashi; Gerdes, Florian; Langer, Thomas; Tsai, Francis T F

    2011-02-11

    FtsH-related AAA proteases are conserved membrane-anchored, ATP-dependent molecular machines, which mediate the processing and turnover of soluble and membrane-embedded proteins in eubacteria, mitochondria, and chloroplasts. Homo- and hetero-oligomeric proteolytic complexes exist, which are composed of homologous subunits harboring an ATPase domain of the AAA family and an H41 metallopeptidase domain. Mutations in subunits of mitochondrial m-AAA proteases have been associated with different neurodegenerative disorders in human, raising questions on the functional differences between homo- and hetero-oligomeric AAA proteases. Here, we have analyzed the hetero-oligomeric yeast m-AAA protease composed of homologous Yta10 and Yta12 subunits. We combined genetic and structural approaches to define the molecular determinants for oligomer assembly and to assess functional similarities between Yta10 and Yta12. We demonstrate that replacement of only two amino acid residues within the metallopeptidase domain of Yta12 allows its assembly into homo-oligomeric complexes. To provide a molecular explanation, we determined the 12 Å resolution structure of the intact yeast m-AAA protease with its transmembrane domains by electron cryomicroscopy (cryo-EM) and atomic structure fitting. The full-length m-AAA protease has a bipartite structure and is a hexamer in solution. We found that residues in Yta12, which facilitate homo-oligomerization when mutated, are located at the interface between neighboring protomers in the hexamer ring. Notably, the transmembrane and intermembrane space domains are separated from the main body, creating a passage on the matrix side, which is wide enough to accommodate unfolded but not folded polypeptides. These results suggest a mechanism regarding how proteins are recognized and degraded by m-AAA proteases.

  13. Dosimetric comparison of peripheral NSCLC SBRT using Acuros XB and AAA calculation algorithms.

    Science.gov (United States)

    Ong, Chloe C H; Ang, Khong Wei; Soh, Roger C X; Tin, Kah Ming; Yap, Jerome H H; Lee, James C L; Bragg, Christopher M

    2017-01-01

    There is a concern for dose calculation in highly heterogenous environments such as the thorax region. This study compares the quality of treatment plans of peripheral non-small cell lung cancer (NSCLC) stereotactic body radiation therapy (SBRT) using 2 calculation algorithms, namely, Eclipse Anisotropic Analytical Algorithm (AAA) and Acuros External Beam (AXB), for 3-dimensional conformal radiation therapy (3DCRT) and volumetric-modulated arc therapy (VMAT). Four-dimensional computed tomography (4DCT) data from 20 anonymized patients were studied using Varian Eclipse planning system, AXB, and AAA version 10.0.28. A 3DCRT plan and a VMAT plan were generated using AAA and AXB with constant plan parameters for each patient. The prescription and dose constraints were benchmarked against Radiation Therapy Oncology Group (RTOG) 0915 protocol. Planning parameters of the plan were compared statistically using Mann-Whitney U tests. Results showed that 3DCRT and VMAT plans have a lower target coverage up to 8% when calculated using AXB as compared with AAA. The conformity index (CI) for AXB plans was 4.7% lower than AAA plans, but was closer to unity, which indicated better target conformity. AXB produced plans with global maximum doses which were, on average, 2% hotter than AAA plans. Both 3DCRT and VMAT plans were able to achieve D95%. VMAT plans were shown to be more conformal (CI = 1.01) and were at least 3.2% and 1.5% lower in terms of PTV maximum and mean dose, respectively. There was no statistically significant difference for doses received by organs at risk (OARs) regardless of calculation algorithms and treatment techniques. In general, the difference in tissue modeling for AXB and AAA algorithm is responsible for the dose distribution between the AXB and the AAA algorithms. The AXB VMAT plans could be used to benefit patients receiving peripheral NSCLC SBRT. Copyright © 2017 American Association of Medical Dosimetrists. Published by Elsevier Inc. All rights

  14. Endovascular abdominal aortic repair for AAA. Anatomical suitability and limitation in Japanese population according to the inclusion criteria of Zenith AAA stent graft

    International Nuclear Information System (INIS)

    Kitagawa, Atsushi; Okita, Yutaka; Okada, Kenji

    2009-01-01

    Since 2007, the EVAR (endovascular abdominal aortic repair) grafts, Zenith, Excluder and Powerlink had been commercially available in Japan. However, a small iliac artery, typical of Japanese population especially in women, was a limiting factor to indicate EVAR. We analyzed the suitability of EVAR in Japanese population according to the inclusion criteria of Zenith AAA stent graft in the current study. From January 2006 to December 2007, 106 AAA (abdominal aortic aneurysm) patients (88 men, 18 women) with a mean age of 73 years were investigated in our institution by multi-slice CT scan in terms of suitability of EVAR, then we measured their abdominal aorta and iliac artery parameters as follows; proximal neck diameter (PND) and length (PNL), common iliac artery diameter (CIAD) and length (CIAL), suprarenal (SNA) and infrarenal neck angulation (INA), external iliac artery diameter (EIAD) and aortic length from the lowest renal artery to the aortic bifurcation (AOL). The inclusion criteria for Zenith AAA stent graft treatment were; PND: 18-28 mm, PNL more than 15 mm, unilateral CIAD less than 20 mm, CIAL at least 10 mm, SNA less than 45 degree and INA less than 60 degree, unilateral EIAD more than 7.5 mm. The indication of EVAR was 25.5% (27/106 patients), and was especially very low in women (5.6%) strictly according to the inclusion criteria of the Zenith AAA stent graft. The main reason of exclusion of EVAR was proximal short neck (40.5%), small iliac artery (30.4%) and infrarenal aortic neck angulation (29.1%). In our analysis, female AAA patients had small PNL and EIAD with angulated neck compared with male AAA ones. Anatomical suitability of EVAR in Japanese population strictly following by the inclusion criteria of Zenith AAA stent graft was low due to their characteristic differences from the European Union (EU) and the United States (US) patients, such as short proximal neck, steep neck angulation and small iliac artery, especially in women. More flexible

  15. The peroxisomal AAA ATPase complex prevents pexophagy and development of peroxisome biogenesis disorders.

    Science.gov (United States)

    Law, Kelsey B; Bronte-Tinkew, Dana; Di Pietro, Erminia; Snowden, Ann; Jones, Richard O; Moser, Ann; Brumell, John H; Braverman, Nancy; Kim, Peter K

    2017-05-04

    Peroxisome biogenesis disorders (PBDs) are metabolic disorders caused by the loss of peroxisomes. The majority of PBDs result from mutation in one of 3 genes that encode for the peroxisomal AAA ATPase complex (AAA-complex) required for cycling PEX5 for peroxisomal matrix protein import. Mutations in these genes are thought to result in a defect in peroxisome assembly by preventing the import of matrix proteins. However, we show here that loss of the AAA-complex does not prevent matrix protein import, but instead causes an upregulation of peroxisome degradation by macroautophagy, or pexophagy. The loss of AAA-complex function in cells results in the accumulation of ubiquitinated PEX5 on the peroxisomal membrane that signals pexophagy. Inhibiting autophagy by genetic or pharmacological approaches rescues peroxisome number, protein import and function. Our findings suggest that the peroxisomal AAA-complex is required for peroxisome quality control, whereas its absence results in the selective degradation of the peroxisome. Thus the loss of peroxisomes in PBD patients with mutations in their peroxisomal AAA-complex is a result of increased pexophagy. Our study also provides a framework for the development of novel therapeutic treatments for PBDs.

  16. Determining the influence of calcification on the failure properties of abdominal aortic aneurysm (AAA) tissue.

    Science.gov (United States)

    O'Leary, Siobhan A; Mulvihill, John J; Barrett, Hilary E; Kavanagh, Eamon G; Walsh, Michael T; McGloughlin, Tim M; Doyle, Barry J

    2015-02-01

    Varying degrees of calcification are present in most abdominal aortic aneurysms (AAAs). However, their impact on AAA failure properties and AAA rupture risk is unclear. The aim of this work is evaluate and compare the failure properties of partially calcified and predominantly fibrous AAA tissue and investigate the potential reasons for failure. Uniaxial mechanical testing was performed on AAA samples harvested from 31 patients undergoing open surgical repair. Individual tensile samples were divided into two groups: fibrous (n=31) and partially calcified (n=38). The presence of calcification was confirmed by fourier transform infrared spectroscopy (FTIR). A total of 69 mechanical tests were performed and the failure stretch (λf), failure stress (σf) and failure tension (Tf) were recorded for each test. Following mechanical testing, the failure sites of a subset of both tissue types were examined using scanning electron microscopy (SEM)/energy dispersive X-ray spectroscopy (EDS) to investigate the potential reasons for failure. It has been shown that the failure properties of partially calcified tissue are significantly reduced compared to fibrous tissue and SEM and EDS results suggest that the junction between a calcification deposit and the fibrous matrix is highly susceptible to failure. This study implicates the presence of calcification as a key player in AAA rupture risk and provides further motivation for the development of non-invasive methods of measuring calcification. Copyright © 2014 Elsevier Ltd. All rights reserved.

  17. The m-AAA Protease Associated with Neurodegeneration Limits MCU Activity in Mitochondria.

    Science.gov (United States)

    König, Tim; Tröder, Simon E; Bakka, Kavya; Korwitz, Anne; Richter-Dennerlein, Ricarda; Lampe, Philipp A; Patron, Maria; Mühlmeister, Mareike; Guerrero-Castillo, Sergio; Brandt, Ulrich; Decker, Thorsten; Lauria, Ines; Paggio, Angela; Rizzuto, Rosario; Rugarli, Elena I; De Stefani, Diego; Langer, Thomas

    2016-10-06

    Mutations in subunits of mitochondrial m-AAA proteases in the inner membrane cause neurodegeneration in spinocerebellar ataxia (SCA28) and hereditary spastic paraplegia (HSP7). m-AAA proteases preserve mitochondrial proteostasis, mitochondrial morphology, and efficient OXPHOS activity, but the cause for neuronal loss in disease is unknown. We have determined the neuronal interactome of m-AAA proteases in mice and identified a complex with C2ORF47 (termed MAIP1), which counteracts cell death by regulating the assembly of the mitochondrial Ca 2+ uniporter MCU. While MAIP1 assists biogenesis of the MCU subunit EMRE, the m-AAA protease degrades non-assembled EMRE and ensures efficient assembly of gatekeeper subunits with MCU. Loss of the m-AAA protease results in accumulation of constitutively active MCU-EMRE channels lacking gatekeeper subunits in neuronal mitochondria and facilitates mitochondrial Ca 2+ overload, mitochondrial permeability transition pore opening, and neuronal death. Together, our results explain neuronal loss in m-AAA protease deficiency by deregulated mitochondrial Ca 2+ homeostasis. Copyright © 2016 Elsevier Inc. All rights reserved.

  18. An AAA-DDD triply hydrogen-bonded complex easily accessible for supramolecular polymers.

    Science.gov (United States)

    Han, Yi-Fei; Chen, Wen-Qiang; Wang, Hong-Bo; Yuan, Ying-Xue; Wu, Na-Na; Song, Xiang-Zhi; Yang, Lan

    2014-12-15

    For a complementary hydrogen-bonded complex, when every hydrogen-bond acceptor is on one side and every hydrogen-bond donor is on the other, all secondary interactions are attractive and the complex is highly stable. AAA-DDD (A=acceptor, D=donor) is considered to be the most stable among triply hydrogen-bonded sequences. The easily synthesized and further derivatized AAA-DDD system is very desirable for hydrogen-bonded functional materials. In this case, AAA and DDD, starting from 4-methoxybenzaldehyde, were synthesized with the Hantzsch pyridine synthesis and Friedländer annulation reaction. The association constant determined by fluorescence titration in chloroform at room temperature is 2.09×10(7)  M(-1) . The AAA and DDD components are not coplanar, but form a V shape in the solid state. Supramolecular polymers based on AAA-DDD triply hydrogen bonded have also been developed. This work may make AAA-DDD triply hydrogen-bonded sequences easily accessible for stimuli-responsive materials. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. The Mitochondrial m-AAA Protease Prevents Demyelination and Hair Greying.

    Science.gov (United States)

    Wang, Shuaiyu; Jacquemyn, Julie; Murru, Sara; Martinelli, Paola; Barth, Esther; Langer, Thomas; Niessen, Carien M; Rugarli, Elena I

    2016-12-01

    The m-AAA protease preserves proteostasis of the inner mitochondrial membrane. It ensures a functional respiratory chain, by controlling the turnover of respiratory complex subunits and allowing mitochondrial translation, but other functions in mitochondria are conceivable. Mutations in genes encoding subunits of the m-AAA protease have been linked to various neurodegenerative diseases in humans, such as hereditary spastic paraplegia and spinocerebellar ataxia. While essential functions of the m-AAA protease for neuronal survival have been established, its role in adult glial cells remains enigmatic. Here, we show that deletion of the highly expressed subunit AFG3L2 in mature mouse oligodendrocytes provokes early-on mitochondrial fragmentation and swelling, as previously shown in neurons, but causes only late-onset motor defects and myelin abnormalities. In contrast, total ablation of the m-AAA protease, by deleting both Afg3l2 and its paralogue Afg3l1, triggers progressive motor dysfunction and demyelination, owing to rapid oligodendrocyte cell death. Surprisingly, the mice showed premature hair greying, caused by progressive loss of melanoblasts that share a common developmental origin with Schwann cells and are targeted in our experiments. Thus, while both neurons and glial cells are dependant on the m-AAA protease for survival in vivo, complete ablation of the complex is necessary to trigger death of oligodendrocytes, hinting to cell-autonomous thresholds of vulnerability to m-AAA protease deficiency.

  20. Abdominal aorta aneurysm (AAA): Is there a role for prevention and therapy using antioxidants?

    Science.gov (United States)

    Pincemail, Joël; Defraigne, Jean-Olivier; Courtois, Audrey; Albert, Adelin; Cheramy-Bien, Jean-Paul; Sakalihasan, Natzi

    2017-09-18

    Abdominal aortic aneurysm (AAA) is a degenerative disease that cause mortality in people aged > 65 years. Increased reactive oxygen species (ROS) and oxidative stress seems to play a pivotal role in AAA pathogenesis. Several sources of ROS have been identified in aortic tissues using experimental models: inflammation, increased activity of NAD(P)H or NOX, over-expression of inducible nitric oxide synthase (iNOS), uncoupled endothelial nitric oxide synthase (eNOS), platelets activation and iron release from hemoglobin. Reducing oxidative stress by antioxidants has been shown to be a potential strategy for limiting AAA development. Human studies confirmed that oxidative stress and endothelial dysfunction are well associated with AAA development. Unfortunately, there is currently no evidence showing that strategies using low molecular weight antioxidants (vitamins C and E, β-carotene) as target for ROS is effective to reduce human AAA progression. However, recent epidemiological data have highlighted the positive role of a diet enriched in fruits which contain high amounts of antioxidant polyphenols. By their ability to restore endothelial function but also their capacity to stimulate enzymatic antioxidants trough activation of the Keap1/Nrf2/ARE pathway, polyphenols can represent a promising treatment target for reducing human AAA progression. Clinical studies are therefore urgently necessary to confirm such a suggestion. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  1. Clinical implementation of AXB from AAA for breast: Plan quality and subvolume analysis.

    Science.gov (United States)

    Guebert, Alexandra; Conroy, Leigh; Weppler, Sarah; Alghamdi, Majed; Conway, Jessica; Harper, Lindsay; Phan, Tien; Olivotto, Ivo A; Smith, Wendy L; Quirk, Sarah

    2018-04-25

    Two dose calculation algorithms are available in Varian Eclipse software: Anisotropic Analytical Algorithm (AAA) and Acuros External Beam (AXB). Many Varian Eclipse-based centers have access to AXB; however, a thorough understanding of how it will affect plan characteristics and, subsequently, clinical practice is necessary prior to implementation. We characterized the difference in breast plan quality between AXB and AAA for dissemination to clinicians during implementation. Locoregional irradiation plans were created with AAA for 30 breast cancer patients with a prescription dose of 50 Gy to the breast and 45 Gy to the regional node, in 25 fractions. The internal mammary chain (IMC CTV ) nodes were covered by 80% of the breast dose. AXB, both dose-to-water and dose-to-medium reporting, was used to recalculate plans while maintaining constant monitor units. Target coverage and organ-at-risk doses were compared between the two algorithms using dose-volume parameters. An analysis to assess location-specific changes was performed by dividing the breast into nine subvolumes in the superior-inferior and left-right directions. There were minimal differences found between the AXB and AAA calculated plans. The median difference between AXB and AAA for breast CTV V 95% , was AAA for breast radiotherapy is not expected to result in changes in clinical practice for prescribing or planning breast radiotherapy. © 2018 The Authors. Journal of Applied Clinical Medical Physics published by Wiley Periodicals, Inc. on behalf of American Association of Physicists in Medicine.

  2. The AAA+ ATPase p97, a cellular multitool.

    Science.gov (United States)

    Stach, Lasse; Freemont, Paul S

    2017-08-17

    The AAA+ (ATPases associated with diverse cellular activities) ATPase p97 is essential to a wide range of cellular functions, including endoplasmic reticulum-associated degradation, membrane fusion, NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) activation and chromatin-associated processes, which are regulated by ubiquitination. p97 acts downstream from ubiquitin signaling events and utilizes the energy from ATP hydrolysis to extract its substrate proteins from cellular structures or multiprotein complexes. A multitude of p97 cofactors have evolved which are essential to p97 function. Ubiquitin-interacting domains and p97-binding domains combine to form bi-functional cofactors, whose complexes with p97 enable the enzyme to interact with a wide range of ubiquitinated substrates. A set of mutations in p97 have been shown to cause the multisystem proteinopathy inclusion body myopathy associated with Paget's disease of bone and frontotemporal dementia. In addition, p97 inhibition has been identified as a promising approach to provoke proteotoxic stress in tumors. In this review, we will describe the cellular processes governed by p97, how the cofactors interact with both p97 and its ubiquitinated substrates, p97 enzymology and the current status in developing p97 inhibitors for cancer therapy. © 2017 The Author(s).

  3. Radiation dose assessment of musa acuminata - triploid (AAA)

    International Nuclear Information System (INIS)

    Maravillas, Mart Andrew S.; Locaylocay, Jocelyn R.; Mendoza, Concepcion S.

    2008-01-01

    Bananas are radioactive due to the presence of the radioisotope- 40 K. This imposes a possible health risk to the general public. This study intended to assess the annual equivalent dosages and the annual effective dosage committed by the body. This seeks to benefit the general public, students and researchers, and entrepreneurs. Using atomic absorption spectrophotometry, lakatan banana (Musa acuminata-triploid (AAA), the most purchased variety cultivated in Barangay Adlawon, Cebu City, Philippines, was found to contain 0.53 g of total potassium for every 100 g of its fresh fruit wherein 6.2 x 10 -5 g of which is potassium-40. Based on its 40 K content banana was calculated to have a radioactivity of 16 Bq/100 g. it was found out that the body is exposed to radiation dosages ranging from 2.8 x 10 -3 rem annually by eating 100 g of lakatan bananas everyday. Conversely, it is equivalent to the annual effective dosage of 0.0043 rem; the amount at which the body of an individual is uniformly exposed. However, no or extremely minute health risk was determined by just eating bananas. In fact, to exceed the radiation dose limits set by the International Commission on Radiation Protection, an individual may eat 116 kg of lakatan bananas everyday for a year. Fertilizers may be the major source of the radioisotope - 40 K and assimilated by the plants. (author)

  4. Democracy and "Grand" Corruption.

    Science.gov (United States)

    Rose-Ackerman, Susan

    1996-01-01

    Defines "grand" corruption as that occurring at the higher levels of a political system and involving large sums of money. Discusses the impact and incentives for this level of corruption as well as various government responses. Identifies multinational corporations as the major malefactors. (MJP)

  5. The GRANDE detector

    International Nuclear Information System (INIS)

    Adams, A.; Bond, R.; Coleman, L.; Rollefson, A.; Wold, D.; Bratton, C.B.; Gurr, H.; Kropp, W.; Nelson, M.; Price, L.R.; Reines, F.; Schultz, J.; Sobel, H.; Svoboda, R.; Yodh, G.; Burnett, T.; Chaloupka, V.; Wilkes, R.J.; Cherry, M.; Ellison, S.B.; Guzik, T.G.; Wefel, J.; Gaidos, J.; Loeffler, F.; Sembroski, G.; Wilson, C.; Goodman, J.; Haines, T.J.; Kielczewska, D.; Lane, C.; Steinberg, R.; Lieber, M.; Nagle, D.; Potter, M.; Tripp, R.

    1990-01-01

    In this paper we present a detector facility which meets the requirements outlined above for a next-generation instrument. GRANDE (Gamma Ray and Neutrino DEtector) is an imaging, water Cerenkov detector, which combines in one facility an extensive air shower array and a high-energy neutrino detector. (orig.)

  6. Triggering the GRANDE array

    International Nuclear Information System (INIS)

    Wilson, C.L.; Bratton, C.B.; Gurr, J.; Kropp, W.; Nelson, M.; Sobel, H.; Svoboda, R.; Yodh, G.; Burnett, T.; Chaloupka, V.; Wilkes, R.J.; Cherry, M.; Ellison, S.B.; Guzik, T.G.; Wefel, J.; Gaidos, J.; Loeffler, F.; Sembroski, G.; Goodman, J.; Haines, T.J.; Kielczewska, D.; Lane, C.; Steinberg, R.; Lieber, M.; Nagle, D.; Potter, M.; Tripp, R.

    1990-01-01

    A brief description of the Gamma Ray And Neutrino Detector Experiment (GRANDE) is presented. The detector elements and electronics are described. The trigger logic for the array is then examined. The triggers for the Gamma Ray and the Neutrino portions of the array are treated separately. (orig.)

  7. Grand-Bassam

    African Journals Online (AJOL)

    Geo

    l'estuaire du fleuve Comoé (Grand-Bassam, Côte d'Ivoire). Kouassi Laurent ADOPO1*, Apie Colette AKOBE1, Etche Mireille AMANI2,. Sylvain MONDE3 et Kouamé AKA3. (1)Laboratoire de Géologie Marine, Sédimentologie et Environnement, Centre de Recherche en Ecologie,. Université Felix Houphouet Boigny Abidjan, ...

  8. m-AAA and i-AAA complexes coordinate to regulate OMA1, the stress-activated supervisor of mitochondrial dynamics.

    Science.gov (United States)

    Consolato, Francesco; Maltecca, Francesca; Tulli, Susanna; Sambri, Irene; Casari, Giorgio

    2018-04-09

    The proteolytic processing of dynamin-like GTPase OPA1, mediated by the activity of both YME1L1 [intermembrane (i)-AAA protease complex] and OMA1, is a crucial step in the regulation of mitochondrial dynamics. OMA1 is a zinc metallopeptidase of the inner mitochondrial membrane that undergoes pre-activating proteolytic and auto-proteolytic cleavage after mitochondrial import. Here, we identify AFG3L2 [matrix (m) - AAA complex] as the major protease mediating this event, which acts by maturing the 60 kDa pre-pro-OMA1 to the 40 kDa pro-OMA1 form by severing the N-terminal portion without recognizing a specific consensus sequence. Therefore, m - AAA and i - AAA complexes coordinately regulate OMA1 processing and turnover, and consequently control which OPA1 isoforms are present, thus adding new information on the molecular mechanisms of mitochondrial dynamics and neurodegenerative diseases affected by these phenomena.This article has an associated First Person interview with the first author of the paper. © 2018. Published by The Company of Biologists Ltd.

  9. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    DEFF Research Database (Denmark)

    Ottosson, R O; Hauer, Anna Karlsson; Behrens, C.F.

    2010-01-01

    to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located...... centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6MV. AAA and MC both predict similar perturbations in dose...... distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement...

  10. On the Impact of Intraluminal Thrombus Mechanical Behavior in AAA Passive Mechanics.

    Science.gov (United States)

    Riveros, Fabián; Martufi, Giampaolo; Gasser, T Christian; Rodriguez-Matas, Jose F

    2015-09-01

    Intraluminal thrombus (ILT) is a pseudo-tissue that develops from coagulated blood, and is found in most abdominal aortic aneurysms (AAAs) of clinically relevant size. A number of studies have suggested that ILT mechanical characteristics may be related to AAA risk of rupture, even though there is still great controversy in this regard. ILT is isotropic and inhomogeneous and may appear as a soft (single-layered) or stiff (multilayered fibrotic) tissue. This paper aims to investigate how ILT constitution and topology influence the magnitude and location of peak wall stress (PWS). In total 21 patient-specific AAAs (diameter 4.2-5.4 cm) were reconstructed from computer tomography images and biomechanically analyzed using state-of-the-art modeling assumptions. Results indicated that PWS correlated stronger with ILT volume (ρ = 0.44, p = 0.05) and minimum thickness of ILT layer (ρ = 0.73, p = 0.001) than with maximum AAA diameter (ρ = 0.05, p = 0.82). On average PWS was 20% (SD 12%) higher for FE models that used soft instead of stiff ILT models (p AAA diameter and was independent from ILT stiffness. In addition, ILT heterogeneity, i.e., the spatial composition of soft and stiff thrombus tissue, can considerably influence stress in the AAA wall. The present study is limited to identification of influential biomechanical factors, and how its findings translate to an AAA rupture risk assessment remains to be explored by clinical studies.

  11. Regionalization of Emergent Vascular Surgery for Patients With Ruptured AAA Improves Outcomes.

    Science.gov (United States)

    Warner, Courtney J; Roddy, Sean P; Chang, Benjamin B; Kreienberg, Paul B; Sternbach, Yaron; Taggert, John B; Ozsvath, Kathleen J; Stain, Steven C; Darling, R Clement

    2016-09-01

    Safe and efficient endovascular aneurysm repair (EVAR) for ruptured abdominal aortic aneurysm (r-AAA) requires advanced infrastructure and surgical expertise not available at all US hospitals. The objective was to assess the impact of regionalizing r-AAA care to centers equipped for both open surgical repair (r-OSR) and EVAR (r-EVAR) by vascular surgeons. A retrospective review of all patients with r-AAA undergoing open or endovascular repair in a 12-hospital region. Patient demographics, transfer status, type of repair, and intraoperative variables were recorded. Outcomes included perioperative morbidity and mortality. Four hundred fifty-one patients with r-AAA were treated from 2002 to 2015. Three hundred twenty-one patients (71%) presented initially to community hospitals (CHs) and 130 (29%) presented to the tertiary medical center (MC). Of the 321 patients presenting to CH, 133 (41%) were treated locally (131 OSR; 2 EVAR) and 188 (59%) were transferred to the MC. In total, 318 patients were treated at the MC (122 OSR; 196 EVAR). At the MC, r-EVAR was associated with a lower mortality rate than r-OSR (20% vs 37%, P = 0.001). Transfer did not influence r-EVAR mortality (20% in r-EVAR presenting to MC vs 20% in r-EVAR transferred, P > 0.2). Overall, r-AAA mortality at the MC was 20% lower than CH (27% vs 46%, P AAA repair to centers equipped for both r-EVAR and r-OSR decreased mortality by approximately 20%. Transfer did not impact the mortality of r-EVAR at the tertiary center. Care of r-AAA in the US should be centralized to centers equipped with available technology and vascular surgeons.

  12. Identifying patients with AAA with the highest risk following endovascular repair.

    Science.gov (United States)

    Cadili, Ali; Turnbull, Robert; Hervas-Malo, Marilou; Ghosh, Sunita; Chyczij, Harold

    2012-08-01

    It has been demonstrated that endovascular repair of arterial disease results in reduced perioperative morbidity and mortality compared to open surgical repair. The rates of complications and need for reinterventions, however, have been found to be higher than that in open repair. The purpose of this study was to identify the predictors of endograft complications and mortality in patients undergoing endovascular abdominal aortic aneurysm (AAA) repair; specifically, our aim was to identify a subset of patients with AAA whose risk of periprocedure mortality was so high that they should not be offered endovascular repair. We undertook a prospective review of patients with AAA receiving endovascular therapy at a single institution. Collected variables included age, gender, date of procedure, indication for procedure, size of aneurysm (where applicable), type of endograft used, presence of rupture, American Society of Anesthesiologists (ASA) class, major medical comorbidities, type of anesthesia (general, epidural, or local), length of intensive care unit (ICU) stay, and length of hospital stay. These factors were correlated with the study outcomes (overall mortality, graft complications, morbidity, and reintervention) using univariate and multivariate logistic regression. A total of 199 patients underwent endovascular AAA repair during the study period. The ICU stay, again, was significantly correlated with the primary outcomes (death and graft complications). In addition, length of hospital stay greater than 3 days, also emerged as a statistically significant predictor of graft complications in this subgroup (P = .024). Survival analysis for patients with AAA revealed that age over 85 years and ICU stay were predictive of decreased survival. Statistical analysis for other subgroups of patients (inflammatory AAA or dissection) was not performed due to the small numbers in these subgroups. Patients with AAA greater than 85 years of age are at a greater risk of mortality

  13. OpenCV for secret agents

    CERN Document Server

    Howse, Joseph

    2015-01-01

    This book is for programmers who want to expand their skills by building fun, smart, and useful systems with OpenCV. The projects are ideal in helping you to think creatively about the uses of computer vision, natural user interfaces, and ubiquitous computers (in your home, car, and hand).

  14. AstroCV: Astronomy computer vision library

    Science.gov (United States)

    González, Roberto E.; Muñoz, Roberto P.; Hernández, Cristian A.

    2018-04-01

    AstroCV processes and analyzes big astronomical datasets, and is intended to provide a community repository of high performance Python and C++ algorithms used for image processing and computer vision. The library offers methods for object recognition, segmentation and classification, with emphasis in the automatic detection and classification of galaxies.

  15. Structural basis for the ATP-independent proteolytic activity of LonB proteases and reclassification of their AAA+ modules.

    Science.gov (United States)

    An, Young Jun; Na, Jung-Hyun; Kim, Myung-Il; Cha, Sun-Shin

    2015-10-01

    Lon proteases degrade defective or denature proteins as well as some folded proteins for the control of cellular protein quality. There are two types of Lon proteases, LonA and LonB. Each consists of two functional components: a protease component and an ATPase associated with various cellular activities (AAA+ module). Here, we report the 2.03 -resolution crystal structure of the isolated AAA+ module (iAAA+ module) of LonB from Thermococcus onnurineus NA1 (TonLonB). The iAAA+ module, having no bound nucleotide, adopts a conformation virtually identical to the ADP-bound conformation of AAA+ modules in the hexameric structure of TonLonB; this provides insights into the ATP-independent proteolytic activity observed in a LonB protease. Structural comparison of AAA+ modules between LonA and LonB revealed that the AAA+ modules of Lon proteases are separated into two distinct clades depending on their structural features. The AAA+ module of LonB belongs to the -H2 & Ins1 insert clade (HINS clade)- defined for the first time in this study, while the AAA+ module of LonA is a member of the HCLR clade.

  16. EIN3-like gene expression during fruit ripening of Cavendish banana (Musa acuminata cv. Grande naine).

    Science.gov (United States)

    Mbéguié-A-Mbéguié, Didier; Hubert, Olivier; Fils-Lycaon, Bernard; Chillet, Marc; Baurens, Franc-Christophe

    2008-06-01

    Ethylene signal transduction initiates with ethylene binding at receptor proteins and terminates in a transcription cascade involving the EIN3/EIL transcription factors. Here, we have isolated four cDNAs homologs of the Arabidopsis EIN3/EIN3-like gene, MA-EILs (Musa acuminata ethylene insensitive 3-like) from banana fruit. Sequence comparison with other banana EIL gene already registered in the database led us to conclude that, at this day, at least five different genes namely MA-EIL1, MA-EIL2/AB266318, MA-EIL3/AB266319, MA-EIL4/AB266320 and AB266321 exist in banana. Phylogenetic analyses included all banana EIL genes within a same cluster consisting of rice OsEILs, a monocotyledonous plant as banana. However, MA-EIL1, MA-EIL2/AB266318, MA-EIL4/AB266320 and AB266321 on one side, and MA-EIL3/AB266319 on the other side, belong to two distant subclusters. MA-EIL mRNAs were detected in all examined banana tissues but at lower level in peel than in pulp. According to tissues, MA-EIL genes were differentially regulated by ripening and ethylene in mature green fruit and wounding in old and young leaves. MA-EIL2/AB266318 was the unique ripening- and ethylene-induced gene; MA-EIL1, MA-EIL4/Ab266320 and AB266321 genes were downregulated, while MA-EIL3/AB266319 presented an unusual pattern of expression. Interestingly, a marked change was observed mainly in MA-EIL1 and MA-EIL3/Ab266319 mRNA accumulation concomitantly with changes in ethylene responsiveness of fruit. Upon wounding, the main effect was observed in MA-EIL4/AB266320 and AB266321 mRNA levels, which presented a markedly increase in both young and old leaves, respectively. Data presented in this study suggest the importance of a transcriptionally step control in the regulation of EIL genes during banana fruit ripening.

  17. Grand unified theories

    International Nuclear Information System (INIS)

    Langacker, P.

    1981-01-01

    In this talk I discuss the present status of these theories and of their observational and experimental implications. In section II, I briefly review the standard SU 3 sup(c) x SU 2 x U 1 model of the strong and electroweak interactions. Although phenomenologically successful, the standard model leaves many questions unanswered. Some of these questions are addressed by grand unified theories, which are defined and discussed in Section III. The Georgi-Glashow SU 5 model is described, as are theories based on larger groups such as SO 10 , E 6 , or SO 16 . It is emphasized that there are many possible grand unified theories and that it is an experimental problem not only to test the basic ideas but to discriminate between models. (orig./HSI)

  18. Asymptotically safe grand unification

    Energy Technology Data Exchange (ETDEWEB)

    Bajc, Borut [J. Stefan Institute,1000 Ljubljana (Slovenia); Sannino, Francesco [CP-Origins & the Danish IAS, University of Southern Denmark,Campusvej 55, DK-5230 Odense M (Denmark); Université de Lyon, France, Université Lyon 1, CNRS/IN2P3, UMR5822 IPNL,F-69622 Villeurbanne Cedex (France)

    2016-12-28

    Phenomenologically appealing supersymmetric grand unified theories have large gauge representations and thus are not asymptotically free. Their ultraviolet validity is limited by the appearance of a Landau pole well before the Planck scale. One could hope that these theories save themselves, before the inclusion of gravity, by generating an interacting ultraviolet fixed point, similar to the one recently discovered in non-supersymmetric gauge-Yukawa theories. Employing a-maximization, a-theorem, unitarity bounds, as well as positivity of other central charges we nonperturbatively rule out this possibility for a broad class of prime candidates of phenomenologically relevant supersymmetric grand unified theories. We also uncover candidates passing these tests, which have either exotic matter or contain one field decoupled from the superpotential. The latter class of theories contains a model with the minimal matter content required by phenomenology.

  19. Persistent type II endoleak after EVAR: the predictive value of the AAA thrombus volume.

    Science.gov (United States)

    Gallitto, Enrico; Gargiulo, Mauro; Mascoli, Chiara; Freyrie, Antonio; DE Matteis, Massimo; Serra, Carla; Bianchini Massoni, Claudio; Faggioli, Gianluca; Stella, Andrea

    2018-02-01

    Persistent type II endoleaks (ELIIp, ≥6 months) after an endovascular aneurysm repair (EVAR) can be associated with adverse outcomes. The aims of this study are the evaluation of the incidence of ELIIp, their preoperative morphological predictive features (PMF) and the post-EVAR abdominal aortic aneurysm (AAA) evolution in the presence of ELIIp. Patients underwent EVAR between 2008 and 2010 were prospectively collected. Cases with ELIIp (group A: AG) were identified. A control group without ELIIp (group B: BG), homogeneous for clinical characteristics, follow-up timing and methods (CTA and/or CEUS at 6.12 months and yearly thereafter) was retrospectively selected. The PMF evaluated by computed-tomography-angiography (CTA) were: AAA-diameter, number and diameter of AAA efferent patent vessels (EPV), AAA-total volume (TV), AAA-thrombus volume (THV) and TV/THV rate (%VR). Volumes were calculated by the dedicated vessels analysis software. AG and BG were compared. The primary endpoint was to evaluate the incidence of ELIIp. Secondary endpoints were to analyze the relation between PMF and ELIIp and to assess the post-EVAR AAA-evolution in the presence of ELIIp. Between 2008 and 2010, 200 patients underwent EVAR to treat AAA electively. An ELIIp was detected in 35cases (17.5%) (AG). Twenty-seven patients (13.5%) were included in BG. An overall of 62 patients (GA+GB) were analyzed. The mean pre-operative AAA diameter and EPV were 58±11.6 mm and 5.5±1.8 mm, respectively. The mean TV and THV were 187±111.5 cc and 82±75 cc, respectively. The median %VR was 42.3%. ELIIp was correlated to EPV≥6 (χ2, p=.015) and %VR AAA growth post-EVAR. ELIIp is a not rare complication and it could require re-interventions. Our data suggest that VEP≥6 or %VT<40% are risk factors for ELIIp. No PMF was able to predict the ELIIp evolution. The relative high rate of re-interventions, could suggest the need of adjunctive/preventing primary procedures in patients at high-risk for ELIIp.

  20. LA GRANDE DESCENTE

    CERN Multimedia

    The first endcap disc of CMS being lowered slowly and carefully 100 m underground into the experimental cavern. The disc is one of 15 large pieces to make the grand descent.  The uniquely shaped slice, 16 m high, about 50 cm thick weighs 400 tonnes. The two HF that were lowered earlier in November can also be seen in the foreground and background.  

  1. The Limbic-Prefrontal Network Modulated by Electroacupuncture at CV4 and CV12

    Directory of Open Access Journals (Sweden)

    Jiliang Fang

    2012-01-01

    Full Text Available fMRI studies showed that acupuncture could induce hemodynamic changes in brain networks. Many of these studies focused on whether specific acupoints could activate specific brain regions and were often limited to manual acupuncture at acupoints on the limbs. In this fMRI study, we investigated acupuncture's modulation effects on brain functional networks by electroacupuncture (EA at acupoints on the midline of abdomen. Acupoints Guanyuan (CV4 and Zhongwan (CV12 were stimulated in 21 healthy volunteers. The needling sensations, brain activation, and functional connectivity were studied. We found that the limbic-prefrontal functional network was deactivated by EA at CV4 and CV12. More importantly, the local functional connectivity was significantly changed during EA stimulation, and the change persisted during the period after the stimulation. Although minor differences existed, both acupoints similarly modulated the limbic-prefrontal functional network, which is overlapped with the functional circuits associated with emotional and cognitive regulation.

  2. Effect of Sodium Cyanide on Wheat (Triticum durum cv. Altar and T. aestivum cv. Cumhuriyet)

    DEFF Research Database (Denmark)

    Gemici, Meliha; Karshenass, A.M.; Tan, Kit

    2008-01-01

    The effect of sodium cyanide on the morphology of stem, leaves and grain yields of Triticum durum cv. Altar and Triticum aestivum cv. Cumhuriyet grown under glass was studied. Seeds were planted in six different sets of pots containing ordinary garden soil. After formation of the first leaves......, the first set was used as the control and watered using ordinary bottled water sold commercially. The other five sets with T. durum cv. Altar and T. aestivum cv. Cumhuriyet seedlings were additionally watered with various concentrations of sodium cyanide, the test-quantity used being 10-50 mg/L. Growth...... of individual plants was monitored until grain production. It was found that the sodium cyanide concentrations in the feed solutions affected plant stature, with the plants becoming progressively dwarfed with increasing dosage. Anomalies in the morphological and anatomical structure of the plant were also noted...

  3. Adipocytes and abdominal aortic aneurysm: Putative potential role of adipocytes in the process of AAA development.

    Science.gov (United States)

    Kugo, Hirona; Moriyama, Tatsuya; Zaima, Nobuhiro

    2018-01-15

    Background Adipose tissue plays a role in the storage of excess energy as triglycerides (TGs). Excess fat accumulation causes various metabolic and cardiovascular diseases. It has been reported that ectopic fat deposition and excess TG accumulation in non-adipose tissue might be important predictors of cardiometabolic and vascular risk. For example, ectopic fat in perivascular tissue promotes atherosclerotic plaque formation in the arterial wall. Objective Recently, it has been reported that ectopic fat (adipocyte) in the vascular wall of an abdominal aortic aneurysm (AAA) is present in both human and experimental animal models. The pathological significance of adipocytes in the AAA wall has not been fully understood. In this review, we summarized the functions of adipocytes and discussed potential new drugs that target vascular adipocytes for AAA treatment. Result Previous studies suggest that adipocytes in vascular wall play an important role in the development of AAA. Conclusion Adipocytes in the vascular wall could be novel targets for the development of AAA therapeutic drugs. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  4. Roles of conserved arginines in ATP-binding domains of AAA+ chaperone ClpB from Thermus thermophilus.

    Science.gov (United States)

    Yamasaki, Takashi; Nakazaki, Yosuke; Yoshida, Masasuke; Watanabe, Yo-hei

    2011-07-01

    ClpB, a member of the expanded superfamily of ATPases associated with diverse cellular activities (AAA+), forms a ring-shaped hexamer and cooperates with the DnaK chaperone system to reactivate aggregated proteins in an ATP-dependent manner. The ClpB protomer consists of an N-terminal domain, an AAA+ module (AAA-1), a middle domain, and a second AAA+ module (AAA-2). Each AAA+ module contains highly conserved WalkerA and WalkerB motifs, and two arginines (AAA-1) or one arginine (AAA-2). Here, we investigated the roles of these arginines (Arg322, Arg323, and Arg747) of ClpB from Thermus thermophilus in the ATPase cycle and chaperone function by alanine substitution. These mutations did not affect nucleotide binding, but did inhibit the hydrolysis of the bound ATP and slow the threading of the denatured protein through the central pore of the T. thermophilus ClpB ring, which severely impaired the chaperone functions. Previously, it was demonstrated that ATP binding to the AAA-1 module induced motion of the middle domain and stabilized the ClpB hexamer. However, the arginine mutations of the AAA-1 module destabilized the ClpB hexamer, even though ATP-induced motion of the middle domain was not affected. These results indicated that the three arginines are crucial for ATP hydrolysis and chaperone activity, but not for ATP binding. In addition, the two arginines in AAA-1 and the ATP-induced motion of the middle domain independently contribute to the stabilization of the hexamer. © 2011 The Authors Journal compilation © 2011 FEBS.

  5. Role of mitochondrial processing peptidase and AAA proteases in processing of the yeast acetohydroxyacid synthase precursor.

    Science.gov (United States)

    Dasari, Suvarna; Kölling, Ralf

    2016-07-01

    We studied presequence processing of the mitochondrial-matrix targeted acetohydroxyacid synthase (Ilv2). C-terminal 3HA-tagging altered the cleavage pattern from a single step to sequential two-step cleavage, giving rise to two Ilv2-3HA forms (A and B). Both cleavage events were dependent on the mitochondrial processing peptidase (MPP). We present evidence for the involvement of three AAA ATPases, m- and i-AAA proteases, and Mcx1, in Ilv2-3HA processing. Both, precursor to A-form and A-form to B-form cleavage were strongly affected in a ∆yme1 mutant. These defects could be suppressed by overexpression of MPP, suggesting that MPP activity is limiting in the ∆yme1 mutant. Our data suggest that for some substrates AAA ATPases could play an active role in the translocation of matrix-targeted proteins.

  6. The AAA protein spastin possesses two levels of basal ATPase activity.

    Science.gov (United States)

    Fan, Xiangyu; Lin, Zhijie; Fan, Guanghui; Lu, Jing; Hou, Yongfei; Habai, Gulijiazi; Sun, Linyue; Yu, Pengpeng; Shen, Yuequan; Wen, Maorong; Wang, Chunguang

    2018-04-30

    The AAA ATPase spastin is a microtubule-severing enzyme that plays important roles in various cellular events including axon regeneration. Herein, we found that the basal ATPase activity of spastin is negatively regulated by spastin concentration. By determining a spastin crystal structure, we demonstrate the necessity of intersubunit interactions between spastin AAA domains. Neutralization of the positive charges in the microtubule-binding domain (MTBD) of spastin dramatically decreases the ATPase activity at low concentration, although the ATP-hydrolyzing potential is not affected. These results demonstrate that, in addition to the AAA domain, the MTBD region of spastin is also involved in regulating ATPase activity, making interactions between spastin protomers more complicated than expected. © 2018 Federation of European Biochemical Societies.

  7. CV controls from design to operation

    CERN Document Server

    Blanc, D

    2002-01-01

    The cooling and Ventilation (CV) group has emphasised the need to redefine its organisational structure at the end of 98. The main objective of this operation was to ensure the CV group to be more competitive and efficient through the growing tasks of the LHC projects. The main evolution given to this reorganisation is that the new structure is more project oriented and then operates on three distinct axes: Design, Work and Operation. Process control project management requires a complete and early interaction and participation of all the actors involved. This procedure to be efficient and constructive must be considered and performed not only during the design stage but along the project planning phases and must go beyond the completion work including the process control operation activity. The paper explains the present project management for process control. It describes the present constraints and gives suggestions to a different approach to these projects to improve performances and efficiency of a contr...

  8. Increased AAA-TOB3 correlates with lymph node metastasis and advanced stage of lung adenocarcinoma.

    Science.gov (United States)

    Liu, Yanfeng; Bu, Lina; Li, Wei; Wu, Wei; Wang, Shengyu; Diao, Xin; Zhou, Jing; Chen, Guoan; Yang, Shuanying

    2017-07-24

    This study was to investigate the differential mitochondrial protein expressions in human lung adenocarcinoma and provide preliminary data for further exploration of the carcinogenic mechanism. Total proteins of A549 and 16HBE mitochondria were extracted through 2D polyacrylamide gel electrophoresis (2-DE). The differential mitochondria proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and were further confirmed by Western blot, immunoelectron microscopy and immunohistochemistry (IHC) in A549 cells as well as lung adenocarcinoma tissues. A total of 41 differentially expressed protein spots were found in A549 mitochondria. Of them, 15 proteins were highly expressed and 26 proteins were lowly expressed in the mitochondria of A549 (by more than 1.5 times). Among the 15 more highly expressed proteins, AAA-TOB3 (by more than 3 times) was highly expressed in the mitochondria of A549 compared with the 16HBE, by LC-MS/MS identification. High electron density and clear circular colloidal gold-marked AAA-TOB3 particles were observed in the A549 cells via immunoelectron microscopy. Besides, AAA-TOB3 was confirmed to be elevated in lung adenocarcinoma by Western blot and IHC. Moreover, increased AAA-TOB3 correlated with lymph node metastasis and advanced stage of lung adenocarcinoma (pAAA-TOB3 was highly expressed in lung adenocarcinoma, and the up-regulation of AAA-TOB3 correlated with lymph node metastasis and advanced stage of lung adenocarcinoma, which suggested that it could serve as a potential molecular marker for lung adenocarcinoma.

  9. Dosimetric comparison of Acuros XB, AAA, and XVMC in stereotactic body radiotherapy for lung cancer.

    Science.gov (United States)

    Tsuruta, Yusuke; Nakata, Manabu; Nakamura, Mitsuhiro; Matsuo, Yukinori; Higashimura, Kyoji; Monzen, Hajime; Mizowaki, Takashi; Hiraoka, Masahiro

    2014-08-01

    To compare the dosimetric performance of Acuros XB (AXB), anisotropic analytical algorithm (AAA), and x-ray voxel Monte Carlo (XVMC) in heterogeneous phantoms and lung stereotactic body radiotherapy (SBRT) plans. Water- and lung-equivalent phantoms were combined to evaluate the percentage depth dose and dose profile. The radiation treatment machine Novalis (BrainLab AG, Feldkirchen, Germany) with an x-ray beam energy of 6 MV was used to calculate the doses in the composite phantom at a source-to-surface distance of 100 cm with a gantry angle of 0°. Subsequently, the clinical lung SBRT plans for the 26 consecutive patients were transferred from the iPlan (ver. 4.1; BrainLab AG) to the Eclipse treatment planning systems (ver. 11.0.3; Varian Medical Systems, Palo Alto, CA). The doses were then recalculated with AXB and AAA while maintaining the XVMC-calculated monitor units and beam arrangement. Then the dose-volumetric data obtained using the three different radiation dose calculation algorithms were compared. The results from AXB and XVMC agreed with measurements within ± 3.0% for the lung-equivalent phantom with a 6 × 6 cm(2) field size, whereas AAA values were higher than measurements in the heterogeneous zone and near the boundary, with the greatest difference being 4.1%. AXB and XVMC agreed well with measurements in terms of the profile shape at the boundary of the heterogeneous zone. For the lung SBRT plans, AXB yielded lower values than XVMC in terms of the maximum doses of ITV and PTV; however, the differences were within ± 3.0%. In addition to the dose-volumetric data, the dose distribution analysis showed that AXB yielded dose distribution calculations that were closer to those with XVMC than did AAA. Means ± standard deviation of the computation time was 221.6 ± 53.1 s (range, 124-358 s), 66.1 ± 16.0 s (range, 42-94 s), and 6.7 ± 1.1 s (range, 5-9 s) for XVMC, AXB, and AAA, respectively. In the phantom evaluations, AXB and XVMC agreed better with

  10. Norms for multivariate diagnosis of nutrient imbalance in the East African highland bananas (musa spp.aaa)

    NARCIS (Netherlands)

    Wairegi, L.; Asten, van P.

    2011-01-01

    Despite low yields and soil fertility problems, fertilizer use in the East African Highland banana (AAA-EA) production is absent. High fertilizer costs increase the need for site-specific fertilizer recommendations that address deficiencies. This study aimed to derive and compare norms for AAA-EA

  11. Statins: the holy grail of Abdominal Aortic Aneurysm (AAA) growth attenuation? A systematic review of the literature.

    Science.gov (United States)

    Dunne, Jonathan A; Bailey, Marc A; Griffin, Kathryn J; Sohrabi, Soroush; Coughlin, Patrick A; Scott, D Julian A

    2014-01-01

    In the era of Abdominal Aortic Aneurysm (AAA) screening, pharmacotherapies to attenuate AAA growth are sought. HMG Co-A reductase inhibitors (statins) have pleiotropic actions independent of their lipid lowering effects and have been suggested as potential treatment for small AAAs. We systematically review the clinical evidence for this effect. Medline, EMBASE and the Cochrane Central Register of Controlled Trials (1950-2011) were searched for studies reporting data on the role of statin therapy on AAA growth rate. No language restrictions were placed on the search. References of retrieved articles and pertinent journals were hand searched. Included studies were reviewed by 2 independent observers. The search retrieved 164 papers, 100 were irrelevant based on their title, 47 were reviews and 1 was a letter. 8 studies were excluded based on review of their abstract leaving 8 for inclusion in the study. Eight observational clinical studies with a total of 4,466 patients were reviewed. Four studies demonstrated reduced AAA expansion in statin users while 4 studies failed to demonstrate this effect. The method of determining AAA growth rates varied significantly between the studies and the ability of many studies to control for misclassification bias was poor. The claim that statins attenuate AAA growth remains questionable. Further prospective studies with stringent identification and verification of statin usage and a standardised method of estimating AAA growth rates are required. Statin type and dose also merit consideration.

  12. Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Denis Alcides-Rezende

    2008-01-01

    Full Text Available The objective of this work is to analyse the integration of information systems and information technology resources in the municipal planning of 14 small cities of Rio Grande do Sul (Brazil. The research methodology consisted of a multiple case study together with a convenient non-probabilistic sample chosen through a research protocol. The results demonstrate the difficulties of these cities to organise the municipal data as well as their struggle for accessibility of information and planning for management and control.

  13. EARTH, MOON, SUN, AND CV ACCRETION DISKS

    International Nuclear Information System (INIS)

    Montgomery, M. M.

    2009-01-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting cataclysmic variable (CV) dwarf novae (DN) systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar, and black hole systems. We find that spinning, tilted CV DN systems cannot be described by a precessing ring or by a precessing rigid disk. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our analysis indicates that the best description of a retrogradely precessing spinning, tilted, CV DN accretion disk is a differentially rotating, tilted disk with an attached rotating, tilted ring located near the innermost disk annuli. In agreement with the observations and numerical simulations by others, we find that our numerically simulated CV DN accretion disks retrogradely precess as a unit. Our final, reduced expression for retrograde precession agrees well with our numerical simulation results and with selective observational systems that seem to have main-sequence secondaries. Our results suggest that a major source to retrograde precession is tidal torques like that by the Moon and the Sun on the Earth. In addition, these tidal torques should be common to a variety of systems where one member is spinning and tilted, regardless if

  14. Patient-specific AAA wall stress analysis: 99-percentile versus peak stress

    NARCIS (Netherlands)

    Speelman, L.; Bosboom, E.M.H.; Schurink, G.W.H.; Hellenthal, F.A.M.V.I.; Buth, J.; Breeuwer, M.; Jacobs, M.J.; Vosse, van de F.N.

    2008-01-01

    Objective Biomechanically, rupture of an Abdominal Aortic Aneurysm (AAA) occurs when the stress acting on the wall due to the blood pressure, exceeds the strength of the wall. Peak wall stress estimations, based on CT reconstruction, may be prone to observer variation. This study focuses on the

  15. AAA application in diagnosis exams in a large public hospital, RS, Brazil

    International Nuclear Information System (INIS)

    Bacelar, A.; Ferret, A.A.; Vanni, S.; Galhardi, M.P.; Lykawka, R.

    2013-01-01

    Objective: the initiative AAA - Awareness , Appropriateness and Audit , promotes consciousness ( Awareness) , fitness ( Appropriateness ) and Audit ( Audit) . This paper analyzes the application of the concept in the AAA requests and justifications examinations using ionizing radiation within a large public hospital. Materials and methods: we collected and analyzed data between the years 2011 and 2012, concerning the number of exams performed with the use of radiation and their justifications. After, we sought to raise awareness of the clinical team through training on the risks and benefits of the various modalities of the radiology department and the need to justify the use of ionizing radiation on health. After the data were collected again of test requests for verification of the effectiveness of training. Results: the mean requests that need to be appropriate to the AAA in the last quarter of 2011 was 75 % lower than the average demands of the first quarter, matched against the last two months of 2012 increased by up to four times the number requests that require improvements in relation to the excellent results obtained in July 2012. Conclusion: it is shown in this paper the need of implementing this initiative AAA continuously added to the clinical staff awareness about the risk of the use of ionizing radiation, the appropriateness of the requests of these tests , as well as the control of this process in order to optimize use of ionizing radiation on health

  16. Anonymous Communication Policies for the Internet: Results and Recommendations of the AAAS Conference.

    Science.gov (United States)

    Teich, Al; Frankel, Mark S.; Kling, Rob; Lee, Yaching

    1999-01-01

    Reports the results of a conference on the Internet and anonymous communication organized by the American Association for the Advancement of Science (AAAS). Discusses how anonymous communications can be shaped by the law, education, and public awareness, and highlights the importance of involving all affected interests in policy development.…

  17. Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major

    International Nuclear Information System (INIS)

    Arakaki, Tracy; Le Trong, Isolde; Phizicky, Eric; Quartley, Erin; DeTitta, George; Luft, Joseph; Lauricella, Angela; Anderson, Lori; Kalyuzhniy, Oleksandr; Worthey, Elizabeth; Myler, Peter J.; Kim, David; Baker, David; Hol, Wim G. J.; Merritt, Ethan A.

    2006-01-01

    The crystal structure of a conserved hypothetical protein from L. major, Pfam sequence family PF04543, structural genomics target ID Lmaj006129AAA, has been determined at a resolution of 1.6 Å. The gene product of structural genomics target Lmaj006129 from Leishmania major codes for a 164-residue protein of unknown function. When SeMet expression of the full-length gene product failed, several truncation variants were created with the aid of Ginzu, a domain-prediction method. 11 truncations were selected for expression, purification and crystallization based upon secondary-structure elements and disorder. The structure of one of these variants, Lmaj006129AAH, was solved by multiple-wavelength anomalous diffraction (MAD) using ELVES, an automatic protein crystal structure-determination system. This model was then successfully used as a molecular-replacement probe for the parent full-length target, Lmaj006129AAA. The final structure of Lmaj006129AAA was refined to an R value of 0.185 (R free = 0.229) at 1.60 Å resolution. Structure and sequence comparisons based on Lmaj006129AAA suggest that proteins belonging to Pfam sequence families PF04543 and PF01878 may share a common ligand-binding motif

  18. Improved Resident Adherence to AAA Screening Guidelines via an Electronic Reminder.

    Science.gov (United States)

    Sypert, David; Van Dyke, Kenneth; Dhillon, Namrata; Elliott, John O; Jordan, Kim

    The 2014 United States Preventive Services Task Force systematic review found abdominal aortic aneurysm (AAA) screening decreased related mortality by close to half. Despite the simplicity of screening, research suggests poor adherence to the recommended AAA screening guidelines. Using the quality improvement plan-study-do-act cycle, we retrospectively established poor adherence to AAA screening and poor documentation of smoking history in our resident clinic. An electronic reminder was prospectively implemented into our electronic medical record (EMR) with the goal of improving screening rates. After 1 year, a retrospective chart review was conducted. Comparisons of the pre- and post-electronic reminder intervention data were made using chi-square tests and odds ratios (OR). The purposeful AAA screening rate improved 27.8% during the intervention, 40.3% (95% confidence interval [CI]: 28.6-52.0%) versus 12.5% (95% CI: 3.1-21.9%), p = .002, suggesting patients were more likely to be screened as a result of the electronic reminder, OR = 4.73 (95% CI: 1.77-12.65). This improvement translates to a large effect size, Cohen's d = 0.86 (95% CI: 0.31-1.40). Electronic reminders are a simple EMR addition that can provide evidence-based education while improving adherence rates with preventive health screening measures.

  19. Structure and Function of p97 and Pex1/6 Type II AAA+ Complexes.

    Science.gov (United States)

    Saffert, Paul; Enenkel, Cordula; Wendler, Petra

    2017-01-01

    Protein complexes of the Type II AAA+ (ATPases associated with diverse cellular activities) family are typically hexamers of 80-150 kDa protomers that harbor two AAA+ ATPase domains. They form double ring assemblies flanked by associated domains, which can be N-terminal, intercalated or C-terminal to the ATPase domains. Most prominent members of this family include NSF (N-ethyl-maleimide sensitive factor), p97/VCP (valosin-containing protein), the Pex1/Pex6 complex and Hsp104 in eukaryotes and ClpB in bacteria. Tremendous efforts have been undertaken to understand the conformational dynamics of protein remodeling type II AAA+ complexes. A uniform mode of action has not been derived from these works. This review focuses on p97/VCP and the Pex1/6 complex, which both structurally remodel ubiquitinated substrate proteins. P97/VCP plays a role in many processes, including ER- associated protein degradation, and the Pex1/Pex6 complex dislocates and recycles the transport receptor Pex5 from the peroxisomal membrane during peroxisomal protein import. We give an introduction into existing knowledge about the biochemical and cellular activities of the complexes before discussing structural information. We particularly emphasize recent electron microscopy structures of the two AAA+ complexes and summarize their structural differences.

  20. The mechanical role of thrombus on the growth rate of an abdominal aortic aneurysm (AAA)

    NARCIS (Netherlands)

    Speelman, L.; Schurink, G.W.H.; Bosboom, E.M.H.; Buth, J.; Breeuwer, M.; Vosse, van de F.N.; Jacobs, M.J.H.M.

    2010-01-01

    Objectives: In the decision for surgical repair of abdominal aortic aneurysms (AAAs), the maximum diameter is the main factor. Several studies have concluded that the diameter may not be reliable as rupture risk criterion for the individual patient and wall stress was found to have a higher

  1. Effects of doping and bias voltage on the screening in AAA-stacked trilayer graphene

    Science.gov (United States)

    Mohammadi, Yawar; Moradian, Rostam; Shirzadi Tabar, Farzad

    2014-09-01

    We calculate the static polarization of AAA-stacked trilayer graphene (TLG) and study its screening properties within the random phase approximation (RPA) in all undoped, doped and biased regimes. We find that the static polarization of undoped AAA-stacked TLG is a combination of the doped and undoped single-layer graphene static polarization. This leads to an enhancement of the dielectric background constant along a Thomas-Fermi screening with the Thomas-Fermi wave vector which is independent of carrier concentrations and a 1/r3 power law decay for the long-distance behavior of the screened Coulomb potential. We show that effects of a bias voltage can be taken into account by a renormalization of the interlayer hopping energy to a new bias-voltage-dependent value, indicating screening properties of AAA-stacked TLG can be tuned electrically. We also find that screening properties of doped AAA-stacked TLG, when μ exceeds √{2}γ, are similar to that of doped SLG only depending on doping. While for μ<√{2}γ, its screening properties are combination of SLG and AA-stacked bilayer graphene screening properties and they are determined by doping and the interlayer hopping energy.

  2. Cassini's Grand Finale Overview

    Science.gov (United States)

    Spilker, L. J.

    2017-12-01

    After 13 years in orbit, the Cassini-Huygens Mission to Saturn ended in a science-rich blaze of glory. Cassini sent back its final bits of unique science data on September 15, 2017, as it plunged into Saturn's atmosphere, vaporizing and satisfying planetary protection requirements. Cassini's final phase covered roughly ten months and ended with the first time exploration of the region between the rings and planet. In late 2016 Cassini transitioned to a series of 20 Ring Grazing orbits with peripases just outside Saturn's F ring, providing close flybys of tiny ring moons, including Pan, Daphnis and Atlas, and high-resolution views of Saturn's A and F rings. A final Titan flyby in late April 2017 propelled Cassini across Saturn's main rings and into its Grand Finale orbits. Comprised of 22 orbits, Cassini repeatedly dove between Saturn's innermost rings and upper atmosphere to answer fundamental questions unattainable earlier in the mission. The last orbit turned the spacecraft into the first Saturn atmosphere probe. The Grand Finale orbits provided highest resolution observations of both the rings and Saturn, and in-situ sampling of the ring particle composition, Saturn's atmosphere, plasma, and innermost radiation belts. The gravitational field was measured to unprecedented accuracy, providing information on the interior structure of the planet, winds in the deeper atmosphere, and mass of the rings. The magnetic field provided insight into the physical nature of the magnetic dynamo and structure of the internal magnetic field. The ion and neutral mass spectrometer sampled the upper atmosphere for molecules that escape the atmosphere in addition to molecules originating from the rings. The cosmic dust analyzer directly sampled the composition from different parts of the main rings for the first time. Fields and particles instruments directly measured the plasma environment between the rings and planet. Science highlights and new mysteries collected in the Grand

  3. Learning openCV computer vision with the openCV library

    CERN Document Server

    Bradski, Gary

    2008-01-01

    Learning OpenCV puts you right in the middle of the rapidly expanding field of computer vision. Written by the creators of OpenCV, the widely used free open-source library, this book introduces you to computer vision and demonstrates how you can quickly build applications that enable computers to see" and make decisions based on the data. With this book, any developer or hobbyist can get up and running with the framework quickly, whether it's to build simple or sophisticated vision applications

  4. Grand unification and supergravity

    International Nuclear Information System (INIS)

    Nanopoulos, D.V.

    Grand Unified Theories (GUTs) are very successful, but they suffer from fine-tuning or hierarchy problems. It seems that more symmetry beyond the gauge symmetry is needed and indeed supersymmetric GUTs may provide the correct framework in solving the hierarchy problems. These are reviewed. From the results discussed, it is seen that for the first time in particle physics, gravity seems to play a dominant role. It may be responsible for GUT breaking, SU(2) x U(1) breaking, fermion masses, proton decay and a consistent cosmological picture. Supergravity seems to offer a consistent, effective theory for energies below the Planck scale to N=1 local SUSY but also, in the context of N=8 extended supergravity with a dynamically realized SU(8), there may be a consistent fundamental unified theory of all interactions. (U.K.)

  5. Grand unification: status report

    International Nuclear Information System (INIS)

    Georgi, H.

    1983-01-01

    Grand unification is reviewed with regard to the flavor puzzle and the hierarchy puzzle. Progress in CP and the PQWWKDFS axion is reviewed. The neutrino mass and B-L research, the understanding and assimilation of the language of effective theories (which divide the momentum scale up into regions), with focus on the models, are surveyed. Various unified models are organized according to whether they address the hierarchy puzzle or the flavor puzzle. SU(5), SO(10), E6, and Higgs are considered simple and explicit models. Global symmetry addresses hierarchy puzzle, but the rules are unclear. In SO (18), with regard to hierarchy, perturbation theory breaks down. SO (14) fails for hierarchy because of GIM, b and t problems. Supersymmetry and technicolor with regard to flavor puzzle are questioned. The CP solution of ETC and Composite C models (addressing both flavor and hierarchy) is a minus. Composite A model has no evident virtues, and the basic idea of ETC model needs checking

  6. Grand unified theories. Pt. 2

    International Nuclear Information System (INIS)

    Ellis, J.

    1982-01-01

    The author gives an introduction to the construction of grand unified theories on the base of the SU(3)xSU(2)xU(1) model of the strong, weak, and electromagnetic interactions. Especially he discusses the proton decay, neutrino masses and oscillations, and cosmological implications in connection with grand unified theories. (orig./HSI)

  7. La importancia de ser grande

    OpenAIRE

    Baisre, J. A.

    2007-01-01

    Se responde a las preguntas ¿por qué los mamíferos marinos son los animales más grandes del planeta?, ¿Por qué los peces no pueden ser más grandes?. Éstas y otras interrogantes son respondidas de forma sencilla y clara.

  8. SOFIA Technology: The NASA Airborne Astronomy Ambassador (AAA) Experience and Online Resources

    Science.gov (United States)

    Clark, C.; Harman, P. K.; Backman, D. E.

    2016-12-01

    SOFIA, an 80/20 partnership of NASA and the German Aerospace Center (DLR), consists of a modified Boeing 747SP carrying a reflecting telescope with an effective diameter of 2.5 meters. SOFIA is the largest airborne observatory in the world, capable of observations impossible for even the largest and highest ground-based telescopes. The SOFIA Program Office is at NASA ARC, Moffett Field, CA; the aircraft is based in Palmdale, CA. During its planned 20-year lifetime, SOFIA will foster development of new scientific instrumentation and inspire the education of young scientists and engineers. Astrophysicists are awarded time on SOFIA to study many kinds of astronomical objects and phenomena. Among the most interesting are: Star birth, evolution, and death Formation of new planetary systems Chemistry of complex molecules in space Planet and exoplanet atmospheres Galactic gas & dust "ecosystems" Environments around supermassive black holes SOFIA currently has eight instruments, five US-made and three German. The instruments — cameras, spectrometers, and a photometer,— operate at near-, mid- and far-infrared wavelengths, each spectral range being best suited to studying particular celestial phenomena. NASA's Airborne Astronomy Ambassadors' (AAAs) experience includes a STEM immersion component. AAAs are onboard during two overnight SOFIA flights that provide insight into the acquisition of scientific data as well as the interfaces between the telescope, instrument, & aircraft. AAAs monitor system performance and view observation targets from their dedicated workstation during flights. Future opportunities for school district partnerships leading to selection of future AAA cohorts will be offered in 2018-19. AAAs may access public archive data via the SOFIA Data Cycle System (DCS) https://dcs.sofia.usra.edu/. Additional SOFIA science and other resources are available at: www.sofia.usra.edu, including lessons that use photovoltaic circuits, and other technology for the

  9. Influencia de la pérdida foliar sobre la cosecha en el cv. Gruesa, Musa acuminata Colla (AAA, cultivado bajo invernadero en las Islas Canarias

    Directory of Open Access Journals (Sweden)

    Juan Cabrera Cabrera

    2010-12-01

    Full Text Available El cultivo de la variedad de platanera Gruesa, selección local de Dwarf Cavendish, ha experimentado un importante aumento en los últimos años en las Islas Canarias, tanto al aire libre como bajo invernadero. La eliminación de hojas, tras la floración, es una práctica habitual en los cultivos bajo invernadero. Asimismo es frecuente la pérdida de hojas por el efecto de los vientos en los cultivos al aire libre. El objetivo de este trabajo es evaluar, mediante simulación de pérdida foliar por daños mecánicos, la influencia que tiene la disminución de superficie foliar sobre el llenado y cosecha de la fruta en dicho cultivar. Para ello, cuatro meses antes de la cosecha se efectuaron cinco niveles de defoliación: 0%, 25%, 50%, 75% y 100%. Se valoran dos métodos diferentes de defoliación, eliminación de limbo foliar y tronchado de hojas con posterior corte de éstas. Se analizan y presentan datos morfológicos, fenológicos y productivos, así como valoración de la metodología empleada en este trabajo para la simulación de daños. A partir de un 25% de defoliado, equivalente a 7.5 hojas funcionales por planta, se detectaron diferencias significativas con las plantas testigos.

  10. SU-F-T-413: Calculation Accuracy of AAA and Acuros Using Cerrobend Blocks for TBI at 400cm

    International Nuclear Information System (INIS)

    Lamichhane, N; Studenski, M

    2016-01-01

    Purpose: It is essential to assess the lung dose during TBI to reduce toxicity. Here we characterize the accuracy of the AAA and Acuros algorithms when using cerrobend lung shielding blocks at an extended distance for TBI. Methods: We positioned a 30×30×30 cm3 solid water slab phantom at 400 cm SSD and measured PDDs (Exradin A12 and PTW parallel plate ion chambers). A 2 cm thick, 10×10 cm2 cerrobend block was hung 2 cm in front of the phantom. This geometry was reproduced in the planning system for both AAA and Acuros. In AAA, the mass density of the cerrobend block was forced to 9.38 g/cm3 and in Acuros it was forced to 8.0 g/cm3 (limited to selecting stainless steel). Three different relative electron densities (RED) were tested for each algorithm; 4.97, 6.97, and 8.97. Results: PDDs from both Acuros and AAA underestimated the delivered dose. AAA calculated that depth dose was higher for RED of 4.97 as compared to 6.97 and 8.97 but still lower than measured. There was no change in the percent depth dose with changing relative electron densities for Acuros. Conclusion: Care should be taken before using AAA or Acuros with cerrobend blocks as the planning system underestimates dose. Acuros limits the ability to modify RED when compared to AAA.

  11. Molecular insights into the m-AAA protease-mediated dislocation of transmembrane helices in the mitochondrial inner membrane.

    Science.gov (United States)

    Lee, Seoeun; Lee, Hunsang; Yoo, Suji; Kim, Hyun

    2017-12-08

    Protein complexes involved in respiration, ATP synthesis, and protein import reside in the mitochondrial inner membrane; thus, proper regulation of these proteins is essential for cell viability. The m -AAA protease, a conserved hetero-hexameric AAA (ATPase associated with diverse cellular activities) protease, composed of the Yta10 and Yta12 proteins, regulates mitochondrial proteostasis by mediating protein maturation and degradation. It also recognizes and mediates the dislocation of membrane-embedded substrates, including foreign transmembrane (TM) segments, but the molecular mechanism involved in these processes remains elusive. This study investigated the role of the TM domains in the m -AAA protease by systematic replacement of one TM domain at a time in yeast. Our data indicated that replacement of the Yta10 TM2 domain abolishes membrane dislocation for only a subset of substrates, whereas replacement of the Yta12 TM2 domain impairs membrane dislocation for all tested substrates, suggesting different roles of the TM domains in each m -AAA protease subunit. Furthermore, m -AAA protease-mediated membrane dislocation was impaired in the presence of a large downstream hydrophilic moiety in a membrane substrate. This finding suggested that the m -AAA protease cannot dislocate large hydrophilic domains across the membrane, indicating that the membrane dislocation probably occurs in a lipid environment. In summary, this study highlights previously underappreciated biological roles of TM domains of the m -AAA proteases in mediating the recognition and dislocation of membrane-embedded substrates. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  12. A conserved inter-domain communication mechanism regulates the ATPase activity of the AAA-protein Drg1.

    Science.gov (United States)

    Prattes, Michael; Loibl, Mathias; Zisser, Gertrude; Luschnig, Daniel; Kappel, Lisa; Rössler, Ingrid; Grassegger, Manuela; Hromic, Altijana; Krieger, Elmar; Gruber, Karl; Pertschy, Brigitte; Bergler, Helmut

    2017-03-17

    AAA-ATPases fulfil essential roles in different cellular pathways and often act in form of hexameric complexes. Interaction with pathway-specific substrate and adaptor proteins recruits them to their targets and modulates their catalytic activity. This substrate dependent regulation of ATP hydrolysis in the AAA-domains is mediated by a non-catalytic N-terminal domain. The exact mechanisms that transmit the signal from the N-domain and coordinate the individual AAA-domains in the hexameric complex are still the topic of intensive research. Here, we present the characterization of a novel mutant variant of the eukaryotic AAA-ATPase Drg1 that shows dysregulation of ATPase activity and altered interaction with Rlp24, its substrate in ribosome biogenesis. This defective regulation is the consequence of amino acid exchanges at the interface between the regulatory N-domain and the adjacent D1 AAA-domain. The effects caused by these mutations strongly resemble those of pathological mutations of the AAA-ATPase p97 which cause the hereditary proteinopathy IBMPFD (inclusion body myopathy associated with Paget's disease of the bone and frontotemporal dementia). Our results therefore suggest well conserved mechanisms of regulation between structurally, but not functionally related members of the AAA-family.

  13. Evaluating the calculation accuracy of AAA algorithm for the situation with small fields in bone by monte carlo

    International Nuclear Information System (INIS)

    Zhang Yanqiu; Qiu Xiaoping; Yang Zhen; Lu Zhiping

    2011-01-01

    In order to evaluate the calculation accuracy of Anisotropic Analytical Algorithm (AAA) for the situation with small fields in a water-bone phantom using Monte Carlo simulation as benchmarks. A water phantom with a bone slab was built,in which the depth dose (DD) and off-axis ratio (OAR) for field 2 cm x 2 cm to field 8 cm x 8 cm were calculated by AAA algorithms, PBC algorithms (as comparison), and Monte Carlo (MC) simulation. The evaluation of algorithms by MC simulation was achieved by the comparisons of DD and the 1 dimension gamma analysis of OAR. It was shown that both of AAA algorithm and PBC algorithm overestimated the DD in bone region, and the dose differences ranged from 2.16%-2.7%, 1.4%-2.03%, respectively. AAA algorithm and PBC algorithm underestimated the DD in back of bone region, and the dose differences ranged from -0.39% - -1.19%, -0.13% - -0.4%, respectively. AAA algorithm and PBC algorithm overestimated the dose of field inner edge and field outer edge,respectively. One dimension gamma analysis indicated that AAA algorithm and PBC algorithm gamma pass rate was 100%, 100%, 100%, 86%, 100%, 100%, 72%, 64%, respectively. In bone medium,the dose calculated by AAA was slightly higher than MC simulation, the calculation accuracy was not evidently higher than PBC. (authors)

  14. SU-F-T-413: Calculation Accuracy of AAA and Acuros Using Cerrobend Blocks for TBI at 400cm

    Energy Technology Data Exchange (ETDEWEB)

    Lamichhane, N; Studenski, M [University of Miami, Miami, FL (United States)

    2016-06-15

    Purpose: It is essential to assess the lung dose during TBI to reduce toxicity. Here we characterize the accuracy of the AAA and Acuros algorithms when using cerrobend lung shielding blocks at an extended distance for TBI. Methods: We positioned a 30×30×30 cm3 solid water slab phantom at 400 cm SSD and measured PDDs (Exradin A12 and PTW parallel plate ion chambers). A 2 cm thick, 10×10 cm2 cerrobend block was hung 2 cm in front of the phantom. This geometry was reproduced in the planning system for both AAA and Acuros. In AAA, the mass density of the cerrobend block was forced to 9.38 g/cm3 and in Acuros it was forced to 8.0 g/cm3 (limited to selecting stainless steel). Three different relative electron densities (RED) were tested for each algorithm; 4.97, 6.97, and 8.97. Results: PDDs from both Acuros and AAA underestimated the delivered dose. AAA calculated that depth dose was higher for RED of 4.97 as compared to 6.97 and 8.97 but still lower than measured. There was no change in the percent depth dose with changing relative electron densities for Acuros. Conclusion: Care should be taken before using AAA or Acuros with cerrobend blocks as the planning system underestimates dose. Acuros limits the ability to modify RED when compared to AAA.

  15. Organisation des travaux ST-CV

    CERN Document Server

    Martel, C; CERN. Geneva. ST Division

    2003-01-01

    Débutée il y a deux ans, la phase de construction des installations de refroidissement du LHC a atteint en 2003 son apogée. De nombreux autres projets sont également gérés par le groupe dans divers secteurs du laboratoire. La spécificité du groupe est de réaliser des installations complexes impliquant des spécificités diverses telles qu’aéraulique, hydraulique, électrique, etc. Pour faire face à ces demandes la section travaux du groupe ST CV a adopté une structure capable de réaliser ces divers projets avec un minimum de personnel. La première partie de ce document décrit l’organisation de la section travaux ST CV. Dans une deuxième partie, les auteurs dressent un état des lieux des chantiers par projet en décrivant plus particulièrement les activités dans les domaines du traitement d’air et de refroidissement par eau.

  16. Retendering of ST-CV maintenance contract

    CERN Document Server

    Battistin, M

    2003-01-01

    During the next three years, the ST/CV group will be deeply involved in the installation works for the LHC project. During this period the need for maintenance activity will decrease. The minimum level will be reached during the “long shutdown” of the PS and SPS machines in 2005. The budget for the maintenance will decrease accordingly, thus the CV group had to review its maintenance strategy. The new contract, which started on January 1st 2003, has been defined to cope with these workload variations during the next years and to guarantee the minimum maintenance activity on the existing equipment. A lump-sum contract based on a win-win strategy has been discarded. The contractor no longer has to guarantee the performance of the CERN cooling and ventilation systems. A new price list strategy based on performance indicators and penalties has been chosen. The contractor now has to guarantee the performance of every maintenance operation demanded by CERN. This modification obliged the Operation section of the...

  17. Prevalence of abdominal aortic aneurysm (AAA) in a population undergoing computed tomography colonography in Canterbury, New Zealand.

    Science.gov (United States)

    Khashram, M; Jones, G T; Roake, J A

    2015-08-01

    There is compelling level 1 evidence in support of screening men for abdominal aortic aneurysm (AAA) to reduce AAA mortality. However, New Zealand (NZ) lacks data on AAA prevalence, and national screening has not been implemented. The aim of this study was to determine the prevalence of AAA in a population undergoing a computed tomography colonography (CTC) for gastrointestinal symptoms. This was an observational study; all consecutive CTCs performed in three regions of the South Island of NZ over a 4 year period were reviewed. Data on abdominal and thoracic aorta diameters ≥30 mm, and iliac and femoral aneurysms ≥20 mm were recorded. Previous aortic surgical grafts or endovascular stents were also documented. Demographics, survival, and AAA related outcomes were collected and used for analysis. Included were 4,893 scans on 4,644 patients (1,933 men [41.6%], 2,711 women [58.4%]) with a median age of 69.3 years (range 17.0-97.0 years). There were 309 scans on 289 patients (75.4% men) who had either an aneurysm or a previous aortic graft with a median age of 79.6 years (range 57.0-96.0 years). Of these, 223 had a native AAA ≥30 mm. The prevalence of AAA rose with age from 1.3% in men aged 55-64 years, to 9.1% in 65-74 year olds, 16.8% in 75-84 year olds, and 22.0% in ≥85 year olds. The corresponding figures in women were 0.4%, 2%, 3.9%, and 6.2%, respectively. In this observational study, the prevalence of AAA was high and warrants further evaluation. The results acquired help to define a population that may benefit from a national AAA screening programme. Copyright © 2015 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.

  18. Clinical implications in the use of the PBC algorithm versus the AAA by comparison of different NTCP models/parameters

    International Nuclear Information System (INIS)

    Bufacchi, Antonella; Nardiello, Barbara; Capparella, Roberto; Begnozzi, Luisa

    2013-01-01

    Retrospective analysis of 3D clinical treatment plans to investigate qualitative, possible, clinical consequences of the use of PBC versus AAA. The 3D dose distributions of 80 treatment plans at four different tumour sites, produced using PBC algorithm, were recalculated using AAA and the same number of monitor units provided by PBC and clinically delivered to each patient; the consequences of the difference on the dose-effect relations for normal tissue injury were studied by comparing different NTCP model/parameters extracted from a review of published studies. In this study the AAA dose calculation is considered as benchmark data. The paired Student t-test was used for statistical comparison of all results obtained from the use of the two algorithms. In the prostate plans, the AAA predicted lower NTCP value (NTCP AAA ) for the risk of late rectal bleeding for each of the seven combinations of NTCP parameters, the maximum mean decrease was 2.2%. In the head-and-neck treatments, each combination of parameters used for the risk of xerostemia from irradiation of the parotid glands involved lower NTCP AAA , that varied from 12.8% (sd=3.0%) to 57.5% (sd=4.0%), while when the PBC algorithm was used the NTCP PBC ’s ranging was from 15.2% (sd=2.7%) to 63.8% (sd=3.8%), according the combination of parameters used; the differences were statistically significant. Also NTCP AAA regarding the risk of radiation pneumonitis in the lung treatments was found to be lower than NTCP PBC for each of the eight sets of NTCP parameters; the maximum mean decrease was 4.5%. A mean increase of 4.3% was found when the NTCP AAA was calculated by the parameters evaluated from dose distribution calculated by a convolution-superposition (CS) algorithm. A markedly different pattern was observed for the risk relating to the development of pneumonitis following breast treatments: the AAA predicted higher NTCP value. The mean NTCP AAA varied from 0.2% (sd = 0.1%) to 2.1% (sd = 0.3%), while the

  19. Increased galectin-3 levels are associated with abdominal aortic aneurysm progression and inhibition of galectin-3 decrease elastase-induced AAA development

    DEFF Research Database (Denmark)

    Fernandez-García, Carlos-Ernesto; Tarin, Carlos; Roldan-Montero, Raquel

    2017-01-01

    Abdominal aortic aneurysm (AAA) evolution is unpredictable. Moreover, no specific treatment exists for AAA, except surgery to prevent aortic rupture. Galectin-3 has been previously associated with CVD, but its potential role in AAA have not been addressed. Galectin-3 levels were increased in plasma...... of AAA patients (n=225) compared to controls (n=100). Moreover, galectin-3 concentrations were associated with need for surgical repair, independently of potential confounding factors. Galectin-3 mRNA and protein expression were increased in human AAA samples compared to healthy aortas. Experimental AAA...... in mice was induced by aortic elastase perfusion. Mice were treated i.v. with the galectin-3 inhibitor modified citrus pectin (MCP, 10mg/kg, every other day) or saline. Similar to humans, galectin-3 serum and aortic mRNA levels were also increased in elastase-induced AAA mice compared to control mice...

  20. Grand slam on cancer.

    Science.gov (United States)

    Gartrell, Nanette

    2014-01-01

    A winner of 59 Grand Slam championships including a record 9 Wimbledon singles titles, Martina Navratilova is the most successful woman tennis player of the modern era. Martina was inducted into the International Tennis Hall of Fame, named "Tour Player of the Year" seven times by the Women's Tennis Association, declared "Female Athlete of the Year" by the Associated Press, and ranked one of the "Top Forty Athletes of All-Time" by Sports Illustrated. Equally accomplished off the court, Martina is an author, philanthropist, TV commentator, and activist who has dedicated her life to educating people about prejudice and stereotypes. After coming out as a lesbian in 1981, Martina became a tireless advocate of equal rights for lesbian, gay, bisexual, and transgender (LGBT) people, and she has contributed generously to the LGBT community. Martina is the author of seven books, including most recently Shape Your Self: My 6-Step Diet and Fitness Plan to Achieve the Best Shape of your Life, an inspiring guide to healthy living and personal fitness. Martina was diagnosed with breast cancer in 2010.

  1. Magnetic spectrometer Grand Raiden

    International Nuclear Information System (INIS)

    Fujiwara, M.; Akimune, H.; Daito, I.; Fujimura, H.; Fujita, Y.; Hatanaka, K.; Ikegami, H.; Katayama, I.; Nagayama, K.; Matsuoka, N.; Morinobu, S.; Noro, T.; Yoshimura, M.; Sakaguchi, H.; Sakemi, Y.; Tamii, A.; Yosoi, M.

    1999-01-01

    A high-resolution magnetic spectrometer called 'Grand Raiden' is operated at the RCNP ring cyclotron facility in Osaka for nuclear physics studies at intermediate energies. This magnetic spectrometer has excellent ion-optical properties. In the design of the spectrometer, the second-order dispersion matching condition has been taken into account, and almost all the aberration terms such as (x vertical bar θ 3 ), (x vertical bar θφ 2 ), (x vertical bar θ 2 δ) and (x vertical bar θδ 2 ) in a third-order matrix calculation are optimized. A large magnetic rigidity of the spectrometer (K = 1400 MeV) gives a great advantage to measure the charge-exchange ( 3 He, t) reactions at 450 MeV. The ability of the high-resolution measurement has been demonstrated. Various coincidence measurements are performed to study the nuclear structures of highly excited states through decay properties of nuclear levels following nuclear reactions at intermediate energies

  2. Clinical physiology grand rounds.

    Science.gov (United States)

    Richards, Jeremy; Schwartzstein, Richard; Irish, Julie; Almeida, Jacqueline; Roberts, David

    2013-04-01

    Clinical Physiology Grand Rounds (CPGR) is an interactive, case-based conference for medical students designed to: (1) integrate preclinical and clinical learning; (2) promote inductive clinical reasoning; and (3) emphasise students as peer teachers. CPGR specifically encourages mixed learning level student interactions and emphasises the use of concept mapping. We describe the theoretical basis and logistical considerations for an interactive, integrative, mixed-learner environment such as CPGR. In addition, we report qualitative data regarding students' attitudes towards and perceptions of CPGR. Medical students from first to fourth year participate in a monthly, interactive conference. The CPGR was designed to bridge gaps and reinforce linkages between basic science and clinical concepts, and to incorporate interactive vertical integration between preclinical and clinical students. Medical education and content experts use Socratic, interactive teaching methods to develop real-time concept maps to emphasise the presence and importance of linkages across curricula. Student focus groups were held to assess attitudes towards and perceptions of the mixed-learner environment and concept maps in CPGR. Qualitative analyses of focus group transcripts were performed to develop themes and codes describing the students' impressions of CPGR. CPGR is a case-based, interactive conference designed to help students gain an increased appreciation of linkages between basic science and clinical medicine concepts, and an increased awareness of clinical reasoning thought processes. Success is dependent upon explicit attention being given to goals for students' integrated learning. © Blackwell Publishing Ltd 2013.

  3. Intraoperative platelet and plasma improves survival in patients operated for a rAAA: a follow-up evaluation

    DEFF Research Database (Denmark)

    Johansson, Per Ingemar; Swiatek, F.; Jorgensen, L.

    2008-01-01

    OBJECTIVES: Continued haemorrhage remains a significant contributor to mortality in massively transfused patients. We found that early administration of platelets and plasma reduced mortality from 54% to 36% in rAAA patients. The aim of the present evaluation was to evaluate whether reduced...... mortality in rAAA patients related to a pro-active transfusion therapy is maintained. DESIGN: Single-centre observational study. METHODS: Mortality of patients operated for rAAA 2006-07 was compared to that of patients operated 2004-05 (intervention group; n=50) and 2002-04 (control group, n=82). RESULTS......: 64 consecutive patients with rAAA received, similar to the intervention group, more platelets (5 and 4 vs. 0 units, P

  4. SFM-FDTD analysis of triangular-lattice AAA structure: Parametric study of the TEM mode

    Science.gov (United States)

    Hamidi, M.; Chemrouk, C.; Belkhir, A.; Kebci, Z.; Ndao, A.; Lamrous, O.; Baida, F. I.

    2014-05-01

    This theoretical work reports a parametric study of enhanced transmission through annular aperture array (AAA) structure arranged in a triangular lattice. The effect of the incidence angle in addition to the inner and outer radii values on the evolution of the transmission spectra is carried out. To this end, a 3D Finite-Difference Time-Domain code based on the Split Field Method (SFM) is used to calculate the spectral response of the structure for any angle of incidence. In order to work through an orthogonal unit cell which presents the advantage to reduce time and space of computation, special periodic boundary conditions are implemented. This study provides a new modeling of AAA structures useful for producing tunable ultra-compact devices.

  5. Dosimetric comparison of Acuros XB, AAA, and XVMC in stereotactic body radiotherapy for lung cancer

    International Nuclear Information System (INIS)

    Tsuruta, Yusuke; Nakata, Manabu; Higashimura, Kyoji; Nakamura, Mitsuhiro; Matsuo, Yukinori; Monzen, Hajime; Mizowaki, Takashi; Hiraoka, Masahiro

    2014-01-01

    Purpose: To compare the dosimetric performance of Acuros XB (AXB), anisotropic analytical algorithm (AAA), and x-ray voxel Monte Carlo (XVMC) in heterogeneous phantoms and lung stereotactic body radiotherapy (SBRT) plans. Methods: Water- and lung-equivalent phantoms were combined to evaluate the percentage depth dose and dose profile. The radiation treatment machine Novalis (BrainLab AG, Feldkirchen, Germany) with an x-ray beam energy of 6 MV was used to calculate the doses in the composite phantom at a source-to-surface distance of 100 cm with a gantry angle of 0°. Subsequently, the clinical lung SBRT plans for the 26 consecutive patients were transferred from the iPlan (ver. 4.1; BrainLab AG) to the Eclipse treatment planning systems (ver. 11.0.3; Varian Medical Systems, Palo Alto, CA). The doses were then recalculated with AXB and AAA while maintaining the XVMC-calculated monitor units and beam arrangement. Then the dose-volumetric data obtained using the three different radiation dose calculation algorithms were compared. Results: The results from AXB and XVMC agreed with measurements within ±3.0% for the lung-equivalent phantom with a 6 × 6 cm 2 field size, whereas AAA values were higher than measurements in the heterogeneous zone and near the boundary, with the greatest difference being 4.1%. AXB and XVMC agreed well with measurements in terms of the profile shape at the boundary of the heterogeneous zone. For the lung SBRT plans, AXB yielded lower values than XVMC in terms of the maximum doses of ITV and PTV; however, the differences were within ±3.0%. In addition to the dose-volumetric data, the dose distribution analysis showed that AXB yielded dose distribution calculations that were closer to those with XVMC than did AAA. Means ± standard deviation of the computation time was 221.6 ± 53.1 s (range, 124–358 s), 66.1 ± 16.0 s (range, 42–94 s), and 6.7 ± 1.1 s (range, 5–9 s) for XVMC, AXB, and AAA, respectively. Conclusions: In the phantom

  6. Neuromuscular regulation in zebrafish by a large AAA+ ATPase/ubiquitin ligase, mysterin/RNF213

    Science.gov (United States)

    Kotani, Yuri; Morito, Daisuke; Yamazaki, Satoru; Ogino, Kazutoyo; Kawakami, Koichi; Takashima, Seiji; Hirata, Hiromi; Nagata, Kazuhiro

    2015-01-01

    Mysterin (also known as RNF213) is a huge intracellular protein with two AAA+ ATPase modules and a RING finger ubiquitin ligase domain. Mysterin was originally isolated as a significant risk factor for the cryptogenic cerebrovascular disorder moyamoya disease, and was found to be involved in physiological angiogenesis in zebrafish. However, the function and the physiological significance of mysterin in other than blood vessels remain largely unknown, although mysterin is ubiquitously expressed in animal tissues. In this study, we performed antisense-mediated suppression of a mysterin orthologue in zebrafish larvae and revealed that mysterin-deficient larvae showed significant reduction in fast myofibrils and immature projection of primary motoneurons, leading to severe motor deficits. Fast muscle-specific restoration of mysterin expression cancelled these phenotypes, and interestingly both AAA+ ATPase and ubiquitin ligase activities of mysterin were indispensable for proper fast muscle formation, demonstrating an essential role of mysterin and its enzymatic activities in the neuromuscular regulation in zebrafish. PMID:26530008

  7. IJ-OpenCV: Combining ImageJ and OpenCV for processing images in biomedicine.

    Science.gov (United States)

    Domínguez, César; Heras, Jónathan; Pascual, Vico

    2017-05-01

    The effective processing of biomedical images usually requires the interoperability of diverse software tools that have different aims but are complementary. The goal of this work is to develop a bridge to connect two of those tools: ImageJ, a program for image analysis in life sciences, and OpenCV, a computer vision and machine learning library. Based on a thorough analysis of ImageJ and OpenCV, we detected the features of these systems that could be enhanced, and developed a library to combine both tools, taking advantage of the strengths of each system. The library was implemented on top of the SciJava converter framework. We also provide a methodology to use this library. We have developed the publicly available library IJ-OpenCV that can be employed to create applications combining features from both ImageJ and OpenCV. From the perspective of ImageJ developers, they can use IJ-OpenCV to easily create plugins that use any functionality provided by the OpenCV library and explore different alternatives. From the perspective of OpenCV developers, this library provides a link to the ImageJ graphical user interface and all its features to handle regions of interest. The IJ-OpenCV library bridges the gap between ImageJ and OpenCV, allowing the connection and the cooperation of these two systems. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. SU-E-T-538: Evaluation of IMRT Dose Calculation Based on Pencil-Beam and AAA Algorithms.

    Science.gov (United States)

    Yuan, Y; Duan, J; Popple, R; Brezovich, I

    2012-06-01

    To evaluate the accuracy of dose calculation for intensity modulated radiation therapy (IMRT) based on Pencil Beam (PB) and Analytical Anisotropic Algorithm (AAA) computation algorithms. IMRT plans of twelve patients with different treatment sites, including head/neck, lung and pelvis, were investigated. For each patient, dose calculation with PB and AAA algorithms using dose grid sizes of 0.5 mm, 0.25 mm, and 0.125 mm, were compared with composite-beam ion chamber and film measurements in patient specific QA. Discrepancies between the calculation and the measurement were evaluated by percentage error for ion chamber dose and γ〉l failure rate in gamma analysis (3%/3mm) for film dosimetry. For 9 patients, ion chamber dose calculated with AAA-algorithms is closer to ion chamber measurement than that calculated with PB algorithm with grid size of 2.5 mm, though all calculated ion chamber doses are within 3% of the measurements. For head/neck patients and other patients with large treatment volumes, γ〉l failure rate is significantly reduced (within 5%) with AAA-based treatment planning compared to generally more than 10% with PB-based treatment planning (grid size=2.5 mm). For lung and brain cancer patients with medium and small treatment volumes, γ〉l failure rates are typically within 5% for both AAA and PB-based treatment planning (grid size=2.5 mm). For both PB and AAA-based treatment planning, improvements of dose calculation accuracy with finer dose grids were observed in film dosimetry of 11 patients and in ion chamber measurements for 3 patients. AAA-based treatment planning provides more accurate dose calculation for head/neck patients and other patients with large treatment volumes. Compared with film dosimetry, a γ〉l failure rate within 5% can be achieved for AAA-based treatment planning. © 2012 American Association of Physicists in Medicine.

  9. Abo1, a conserved bromodomain AAA?ATPase, maintains global nucleosome occupancy and organisation

    OpenAIRE

    Gal, Csenge; Murton, Heather E; Subramanian, Lakxmi; Whale, Alex J; Moore, Karen M; Paszkiewicz, Konrad; Codlin, Sandra; B?hler, J?rg; Creamer, Kevin M; Partridge, Janet F; Allshire, Robin C; Kent, Nicholas A; Whitehall, Simon K

    2015-01-01

    Maintenance of the correct level and organisation of nucleosomes is crucial for genome function. Here, we uncover a role for a conserved bromodomain AAA-ATPase, Abo1, in the maintenance of nucleosome architecture in fission yeast. Cells lacking abo1+ experience both a reduction and mis-positioning of nucleosomes at transcribed sequences in addition to increased intragenic transcription, phenotypes that are hallmarks of defective chromatin re-establishment behind RNA polymerase II. Abo1 is rec...

  10. Characterization of ATPase activity of the AAA ARC from Bifidobacterium longum subsp. infantis.

    Science.gov (United States)

    Guzmán-Rodríguez, Mabel; de la Rosa, Ana Paulina Barba; Santos, Leticia

    2015-01-01

    Bifidobacteria are considered to be probiotics that exist in the large intestine and are helpful to maintain human health. Oral administration of bifidobacteria may be effective in improving the intestinal flora and environment, stimulating the immune response and possibly preventing cancer. However, for consistent and positive results, further well-controlled studies are urgently needed to describe the basic mechanisms of this microorganism. Analysis of the proteasome-lacking Bifidobacterium longum genome reveals that it possesses a gene, IPR003593 AAA ATPase core, which codes a 56 kDa protein containing one AAA ATPase domain. Phylogenetic classification made by CLANS, positioned this sequence into the ARC divergent branch of the AAA ATPase family of proteins. N-terminal analysis of the sequence indicates this protein is closely related to other ATPases such as the Rhodococcus erythropolis ARC, Archaeoglobus fulgidus PAN, Mycobacterium tuberculosis Mpa and the human proteasomal Rpt1 subunit. This gene was cloned, the full-length recombinant protein was overexpressed in Escherichia coli, purified as a high-molecular size complex and named Bl-ARC. Enzymatic characterization showed that Bl-ARC ATPase is active, Mg(+2)-dependent and sensitive to N-ethylmaleimide. Gene organization positions bl-arc in a region flanked by a cluster of genes that includes pup, dop and pafA genes. These findings point to a possible function as a chaperone in the degradation pathway via pupylation.

  11. Inflammatory cell phenotypes in AAAs: their role and potential as targets for therapy.

    Science.gov (United States)

    Dale, Matthew A; Ruhlman, Melissa K; Baxter, B Timothy

    2015-08-01

    Abdominal aortic aneurysms (AAAs) are characterized by chronic inflammatory cell infiltration. AAA is typically an asymptomatic disease and caused ≈15 000 deaths annually in the United States. Previous studies have examined both human and murine aortic tissue for the presence of various inflammatory cell types. Studies show that in both human and experimental AAAs, prominent inflammatory cell infiltration, such as CD4(+) T cells and macrophages, occurs in the damaged aortic wall. These cells have the ability to undergo phenotypic modulation based on microenvironmental cues, potentially influencing disease progression. Proinflammatory CD4(+) T cells and classically activated macrophages dominate the landscape of aortic infiltrates. The skew to proinflammatory phenotypes alters disease progression and plays a role in causing chronic inflammation. The local cytokine production and presence of inflammatory mediators, such as extracellular matrix breakdown products, influence the uneven balance of the inflammatory infiltrate phenotypes. Understanding and developing new strategies that target the proinflammatory phenotype could provide useful therapeutic targets for a disease with no current pharmacological intervention. © 2015 American Heart Association, Inc.

  12. Identification of a Degradation Signal Sequence within Substrates of the Mitochondrial i-AAA Protease.

    Science.gov (United States)

    Rampello, Anthony J; Glynn, Steven E

    2017-03-24

    The i-AAA protease is a component of the mitochondrial quality control machinery that regulates respiration, mitochondrial dynamics, and protein import. The protease is required to select specific substrates for degradation from among the diverse complement of proteins present in mitochondria, yet the rules that govern this selection are unclear. Here, we reconstruct the yeast i-AAA protease, Yme1p, to examine the in vitro degradation of two intermembrane space chaperone subunits, Tim9 and Tim10. Yme1p degrades Tim10 more rapidly than Tim9 despite high sequence and structural similarity, and loss of Tim10 is accelerated by the disruption of conserved disulfide bonds within the substrate. An unstructured N-terminal region of Tim10 is necessary and sufficient to target the substrate to the protease through recognition of a short phenylalanine-rich motif, and the presence of similar motifs in other small Tim proteins predicts robust degradation by the protease. Together, these results identify the first specific degron sequence within a native i-AAA protease substrate. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Structural Insights into the Allosteric Operation of the Lon AAA+ Protease.

    Science.gov (United States)

    Lin, Chien-Chu; Su, Shih-Chieh; Su, Ming-Yuan; Liang, Pi-Hui; Feng, Chia-Cheng; Wu, Shih-Hsiung; Chang, Chung-I

    2016-05-03

    The Lon AAA+ protease (LonA) is an evolutionarily conserved protease that couples the ATPase cycle into motion to drive substrate translocation and degradation. A hallmark feature shared by AAA+ proteases is the stimulation of ATPase activity by substrates. Here we report the structure of LonA bound to three ADPs, revealing the first AAA+ protease assembly where the six protomers are arranged alternately in nucleotide-free and bound states. Nucleotide binding induces large coordinated movements of conserved pore loops from two pairs of three non-adjacent protomers and shuttling of the proteolytic groove between the ATPase site and a previously unknown Arg paddle. Structural and biochemical evidence supports the roles of the substrate-bound proteolytic groove in allosteric stimulation of ATPase activity and the conserved Arg paddle in driving substrate degradation. Altogether, this work provides a molecular framework for understanding how ATP-dependent chemomechanical movements drive allosteric processes for substrate degradation in a major protein-destruction machine. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. The plant i-AAA protease controls the turnover of an essential mitochondrial protein import component.

    Science.gov (United States)

    Opalińska, Magdalena; Parys, Katarzyna; Murcha, Monika W; Jańska, Hanna

    2018-01-29

    Mitochondria are multifunctional organelles that play a central role in energy metabolism. Owing to the life-essential functions of these organelles, mitochondrial content, quality and dynamics are tightly controlled. Across the species, highly conserved ATP-dependent proteases prevent malfunction of mitochondria through versatile activities. This study focuses on a molecular function of the plant mitochondrial inner membrane-embedded AAA protease (denoted i -AAA) FTSH4, providing its first bona fide substrate. Here, we report that the abundance of the Tim17-2 protein, an essential component of the TIM17:23 translocase (Tim17-2 together with Tim50 and Tim23), is directly controlled by the proteolytic activity of FTSH4. Plants that are lacking functional FTSH4 protease are characterized by significantly enhanced capacity of preprotein import through the TIM17:23-dependent pathway. Taken together, with the observation that FTSH4 prevents accumulation of Tim17-2, our data point towards the role of this i -AAA protease in the regulation of mitochondrial biogenesis in plants. © 2018. Published by The Company of Biologists Ltd.

  15. Functional characterization of fidgetin, an AAA-family protein mutated in fidget mice

    International Nuclear Information System (INIS)

    Yang Yan; Mahaffey, Connie L.; Berube, Nathalie; Nystuen, Arne; Frankel, Wayne N.

    2005-01-01

    The mouse fidget mutation is an autosomal recessive mutation that renders reduced or absent semicircular canals, microphthalmia, and various skeletal abnormalities to affected mice. We previously identified the defective gene which encodes fidgetin, a new member of the ATPases associated with diverse cellular activities (AAA proteins). Here, we report on the subcellular localization of fidgetin as well as that of two closely related proteins, fidgetin-like 1 and fidgetin-like 2. Epitope-tagging and immunostaining revealed that both fidgetin and fidgetin-like 2 were predominantly localized to the nucleus, whereas fidgetin-like 1 was both nuclear and cytoplasmic. Furthermore, deletion studies identified a putative bipartite nuclear localization signal in the middle portion of the fidgetin protein. Since AAA proteins are known to form functional hetero- or homo-hexamers, we used reciprocal immunoprecipitation to examine the potential interaction among these proteins. We found that fidgetin interacted with itself and this specific interaction was abolished when either the N- or C-terminus of the protein was truncated. Taken together, our results suggest that fidgetin is a nuclear AAA-family protein with the potential to form homo-oligomers, thus representing the first step towards the elucidation of fidgetin's cellular function and the disease mechanism in fidget mutant mice

  16. Gadopentate dimeglumine enhanced MR angiography (MRA) for staging AAA: a correlation with DSA and CT

    International Nuclear Information System (INIS)

    Arlart, I.P.; Gerlach, A.; Kolb, M.; Erpenbach, S.; Wuerstlin, S.

    1997-01-01

    Purpose: To evaluate a contrast-enhanced (CE) MRA sequence for staging AAA. Methods: In 24 patients (male=20, female=4, age=44-81 y) with known AAA the abdominal aorta and its branches including the iliac arteries were imaged, using a 3D GRE-FISP sequence (1.5 T, T R /T E /FA=25/6/35, slab=100-140 mm, 32 part., FOV=440-450 mm, matrix=256x256) during an i.v. infusion of 40 ml of gadopentate dimeglumine. In addition, representative axial single slices (2D breathhold FLASH-sequence: T R /T E /FA=82/5/30) were acquired following contrast application. MR-results were correlated with i.a. DSA and CT studies. Results: With CE-MRA, AAA (n=24) and iliac aneurysms (n=17) could be evaluated in all cases (sens.=100%, spec.=100%) including luminal patency and mural thrombus. 50/54 renal arteries could be identified, 4/6 accessory renal arteries (sens=66,6%, spec.=100%), 8/9 renal artery stenoses>50% (sens.=88.8%, spec.=89.3%), 1/1 renal artery occlusion and 7/8 iliac artery stenoses>50% (sens.=87.5%, spec.=97.5%) were depicted correctly. Proximal portion of sup. mes. art. could be detected and evaluated in 21/24 cases. Quantitative determination of therapeutically relevant vascular paramters using MRA was comparable to DSA and CT. (orig./AJ) [de

  17. A pull-back algorithm to determine the unloaded vascular geometry in anisotropic hyperelastic AAA passive mechanics.

    Science.gov (United States)

    Riveros, Fabián; Chandra, Santanu; Finol, Ender A; Gasser, T Christian; Rodriguez, Jose F

    2013-04-01

    Biomechanical studies on abdominal aortic aneurysms (AAA) seek to provide for better decision criteria to undergo surgical intervention for AAA repair. More accurate results can be obtained by using appropriate material models for the tissues along with accurate geometric models and more realistic boundary conditions for the lesion. However, patient-specific AAA models are generated from gated medical images in which the artery is under pressure. Therefore, identification of the AAA zero pressure geometry would allow for a more realistic estimate of the aneurysmal wall mechanics. This study proposes a novel iterative algorithm to find the zero pressure geometry of patient-specific AAA models. The methodology allows considering the anisotropic hyperelastic behavior of the aortic wall, its thickness and accounts for the presence of the intraluminal thrombus. Results on 12 patient-specific AAA geometric models indicate that the procedure is computational tractable and efficient, and preserves the global volume of the model. In addition, a comparison of the peak wall stress computed with the zero pressure and CT-based geometries during systole indicates that computations using CT-based geometric models underestimate the peak wall stress by 59 ± 64 and 47 ± 64 kPa for the isotropic and anisotropic material models of the arterial wall, respectively.

  18. Structural Insights into the Unusually Strong ATPase Activity of the AAA Domain of the Caenorhabditis elegans Fidgetin-like 1 (FIGL-1) Protein*

    Science.gov (United States)

    Peng, Wentao; Lin, Zhijie; Li, Weirong; Lu, Jing; Shen, Yuequan; Wang, Chunguang

    2013-01-01

    The FIGL-1 (fidgetin like-1) protein is a homolog of fidgetin, a protein whose mutation leads to multiple developmental defects. The FIGL-1 protein contains an AAA (ATPase associated with various activities) domain and belongs to the AAA superfamily. However, the biological functions and developmental implications of this protein remain unknown. Here, we show that the AAA domain of the Caenorhabditis elegans FIGL-1 protein (CeFIGL-1-AAA), in clear contrast to homologous AAA domains, has an unusually high ATPase activity and forms a hexamer in solution. By determining the crystal structure of CeFIGL-1-AAA, we found that the loop linking helices α9 and α10 folds into the short helix α9a, which has an acidic surface and interacts with a positively charged surface of the neighboring subunit. Disruption of this charge interaction by mutagenesis diminishes both the ATPase activity and oligomerization capacity of the protein. Interestingly, the acidic residues in helix α9a of CeFIGL-1-AAA are not conserved in other homologous AAA domains that have relatively low ATPase activities. These results demonstrate that the sequence of CeFIGL-1-AAA has adapted to establish an intersubunit charge interaction, which contributes to its strong oligomerization and ATPase activity. These unique properties of CeFIGL-1-AAA distinguish it from other homologous proteins, suggesting that CeFIGL-1 may have a distinct biological function. PMID:23979136

  19. Structural insights into the unusually strong ATPase activity of the AAA domain of the Caenorhabditis elegans fidgetin-like 1 (FIGL-1) protein.

    Science.gov (United States)

    Peng, Wentao; Lin, Zhijie; Li, Weirong; Lu, Jing; Shen, Yuequan; Wang, Chunguang

    2013-10-11

    The FIGL-1 (fidgetin like-1) protein is a homolog of fidgetin, a protein whose mutation leads to multiple developmental defects. The FIGL-1 protein contains an AAA (ATPase associated with various activities) domain and belongs to the AAA superfamily. However, the biological functions and developmental implications of this protein remain unknown. Here, we show that the AAA domain of the Caenorhabditis elegans FIGL-1 protein (CeFIGL-1-AAA), in clear contrast to homologous AAA domains, has an unusually high ATPase activity and forms a hexamer in solution. By determining the crystal structure of CeFIGL-1-AAA, we found that the loop linking helices α9 and α10 folds into the short helix α9a, which has an acidic surface and interacts with a positively charged surface of the neighboring subunit. Disruption of this charge interaction by mutagenesis diminishes both the ATPase activity and oligomerization capacity of the protein. Interestingly, the acidic residues in helix α9a of CeFIGL-1-AAA are not conserved in other homologous AAA domains that have relatively low ATPase activities. These results demonstrate that the sequence of CeFIGL-1-AAA has adapted to establish an intersubunit charge interaction, which contributes to its strong oligomerization and ATPase activity. These unique properties of CeFIGL-1-AAA distinguish it from other homologous proteins, suggesting that CeFIGL-1 may have a distinct biological function.

  20. Business Strategy of CV Jaya Sampurna in Facing Soft Drink Distributor Competition in Bekasi

    Directory of Open Access Journals (Sweden)

    Seri Nurmala

    2015-11-01

    Full Text Available CV Jaya Sampurna is a soft drink distributor in Bekasi. Distributor company has a low profit margin, moreover the product sold is Fast Moving Consumers Goods with a very low profit margin (3.5%–5%. Therefore the company depends on a high volume of sales. Besides, there are many competitors in this industry, thus the business strategy is needed to improve the competitive advantage of the company. Given the conditions,this research was to identify the internal and the external factors, to identify the formulation of business strategy alternative, and to identify the recommendation of functional strategy for CV Jaya Sampurna. Research used descriptive method; and the type of research used was case study. Data were gathered from questionnaires and interview to three member of managers: Director, Operational Manager, and Finance Manager. Data wereanalyzed by using EFE, IFE, SWOT, IE, Grand Strategy Matrix and QSPM. The result of this research implies that the alternative strategy based on QSPM’s result was market penetration

  1. SU-F-T-609: Impact of Dosimetric Variation for Prescription Dose Using Analytical Anisotropic Algorithm (AAA) in Lung SBRT

    Energy Technology Data Exchange (ETDEWEB)

    Kawai, D [Kanagawa Cancer Center, Yokohama, Kanagawa (Japan); Takahashi, R [Cancer Institute Hospital of Japanese Foundation for Cancer Research, Koto, Tokyo (Japan); Kamima, T [Cancer Institute Hospital Japanese Foundation for Cancer Research, Koto, Tokyo (Japan); Baba, H [The National Cancer Center Hospital East, Kshiwa, Chiba (Japan); Yamamoto, T; Kubo, Y [Otemae Hospital, Chuo-ku, Osaka (Japan); Ishibashi, S; Higuchi, Y [Sasebo City General Hospital, Sasebo, Nagasaki (Japan); Tani, K [St Luke’s International Hospital, Tokyo, Tokyo (Japan); Tachibana, H [National Cancer Center, Kashiwa, Chiba (Japan)

    2016-06-15

    Purpose: Actual irradiated prescription dose to patients cannot be verified. Thus, independent dose verification and second treatment planning system are used as the secondary check. AAA dose calculation engine has contributed to lung SBRT. We conducted a multi-institutional study to assess variation of prescription dose for lung SBRT when using AAA in reference to using Acuros XB and Clarkson algorithm. Methods: Six institutes in Japan participated in this study. All SBRT treatments were planed using AAA in Eclipse and Adaptive Convolve (AC) in Pinnacle3. All of the institutes used a same independent dose verification software program (Simple MU Analysis: SMU, Triangle Product, Ishikawa, Japan), which implemented a Clarkson-based dose calculation algorithm using CT image dataset. A retrospective analysis for lung SBRT plans (73 patients) was performed to compute the confidence limit (CL, Average±2SD) in dose between the AAA and the SMU. In one of the institutes, a additional analysis was conducted to evaluate the variations between the AAA and the Acuros XB (AXB). Results: The CL for SMU shows larger systematic and random errors of 8.7±9.9 % for AAA than the errors of 5.7±4.2 % for AC. The variations of AAA correlated with the mean CT values in the voxels of PTV (a correlation coefficient : −0.7) . The comparison of AXB vs. AAA shows smaller systematic and random errors of −0.7±1.7%. The correlation between dose variations for AXB and the mean CT values in PTV was weak (0.4). However, there were several plans with more than 2% deviation of AAPM TG114 (Maximum: −3.3 %). Conclusion: In comparison for AC, prescription dose calculated by AAA may be more variable in lung SBRT patient. Even AXB comparison shows unexpected variation. Care should be taken for the use of AAA in lung SBRT. This research is partially supported by Japan Agency for Medical Research and Development (AMED)

  2. Improving Postharvest Storage of Apple Cv.

    Directory of Open Access Journals (Sweden)

    H. Etemadi Nasab

    2013-06-01

    Full Text Available Apple is one of the important fruit in Iran. according to respiration and ethylene production behaviors, apple classified as a climacteric fruit. Therefore, any treatments, which can inhibit or prevent the action of ethylene, they can increase the postharvest life of horticultural production. 1-methylcyclopropane (1-MCP is one of the chemical which is environmental save and use as a very small concentrations. In this experiment, the effects of 1-MCP at concentrations of 0 (as a control, 0.25, 0.5, 0.75 and 1 µl.l-1 were used in apple cv. "Golab Kohanz", a land race fruits which is cultivated from long times ago in Iran. After the 1-MCP treatment, they were moved to cold room at 2 ºC and RH of ca 90% for 60 days. Samples were removed from the cold room and analysis for physicochemical characteristics. "Golab Kohanz" apples treated with 1-MCP showed improved fruit firmness, titratable acidity (TA, and total soluble solid after 147 days at 2ºC , compared to the control non-treated fruits. Also, treatment with 1-MCP significantly reduced the incidence of superficial scald in "Golab Kohanze". In general, 1-MCP-treated apples delay ripening during storing in cold room and increased shelf life. Overall, the results indicate that 1-MCP has tremendous potential for maintaining apple quality during storage and post storage.

  3. Characterization of the binding specificity of Anguilla anguilla agglutinin (AAA) in comparison to Ulex europaeus agglutinin I (UEA-I).

    Science.gov (United States)

    Baldus, S E; Thiele, J; Park, Y O; Hanisch, F G; Bara, J; Fischer, R

    1996-08-01

    Using immunochemical and immunohistochemical methods, the binding site of Anguilla anguilla agglutinin (AAA) was characterized and compared with the related fucose-specific lectin from Ulex europaeus (UEA-I). In solid-phase enzyme-linked immunoassays, the two lectins recognized Fuc alpha 1-2Gal beta-HSA. AAA additionally cross-reacted with neoglycolipids bearing lacto-N-fucopentaose (LNFP) I [H type 1] and II [Le(a)] and lactodifucotetraose (LDFT) as glycan moieties. UEA-I, on the other hand, bound to a LDFT-derived neoglycolipid but not to the other neoglycolipids tested. Binding of AAA to gastric mucin was competitively neutralized by Le(a)-specific monoclonal antibodies. UEA-I binding, on the other hand, was reduced after co-incubation with H type 2- and Le(y)-specific monoclonal antibodies. According to our results, AAA reacts with fucosylated type 1 chain antigens, whereas UEA-I binds only to the alpha 1-2-fucosylated LDFT-derived neoglycolipid. In immunohistochemical studies, the reactivity of AAA and UEA-I in normal pyloric mucosa from individuals with known Lewis and secretor status was analysed. AAA showed a broad reaction in the superficial pyloric mucosa from secretors and non-secretors, but AAA reactivity was more pronounced in Le(a+b-) individuals. On the other hand, UEA-I stained the superficial pyloric mucosa only from secretor individuals. A staining of deep mucous glands by the lectins was found in all specimens. Both reacted with most human carcinomas of different origin. Slight differences in their binding pattern were observed and may be explained by the different fine-specificities of the lectins.

  4. A practical introduction to computer vision with OpenCV

    CERN Document Server

    Dawson-Howe, Kenneth

    2014-01-01

    Explains the theory behind basic computer vision and provides a bridge from the theory to practical implementation using the industry standard OpenCV libraries Computer Vision is a rapidly expanding area and it is becoming progressively easier for developers to make use of this field due to the ready availability of high quality libraries (such as OpenCV 2).  This text is intended to facilitate the practical use of computer vision with the goal being to bridge the gap between the theory and the practical implementation of computer vision. The book will explain how to use the relevant OpenCV

  5. Family members of patients with abdominal aortic aneurysms are at increased risk for aneurysms: analysis of 618 probands and their families from the Liège AAA Family Study.

    Science.gov (United States)

    Sakalihasan, Natzi; Defraigne, Jean-Olivier; Kerstenne, Marie-Ange; Cheramy-Bien, Jean-Paul; Smelser, Diane T; Tromp, Gerard; Kuivaniemi, Helena

    2014-05-01

    The objectives were to answer the following questions with the help of a well-characterized population in Liège, Belgium: 1) what percentage of patients with abdominal aortic aneurysm (AAA) have a positive family history for AAA? 2) what is the prevalence of AAAs among relatives of patients with AAA? and 3) do familial and sporadic AAA cases differ in clinical characteristics? Patients with unrelated AAA diagnosed at the Cardiovascular Surgery Department, University Hospital of Liège, Belgium, between 1999 and 2012 were invited to the study. A detailed family history was obtained in interviews and recorded using Progeny software. We divided the 618 patients into 2 study groups: group I, 296 patients with AAA (268; 91% men) were followed up with computerized tomography combined with positron emission tomography; and group II, 322 patients with AAA (295; 92% men) whose families were invited to ultrasonographic screening. In the initial interview, 62 (10%) of the 618 patients with AAA reported a positive family history for AAA. Ultrasonographic screening identified 24 new AAAs among 186 relatives (≥50 years) of 144 families yielding a prevalence of 13%. The highest prevalence (25%) was found among brothers. By combining the number of AAAs found by ultrasonographic screening with those diagnosed previously the observed lifetime prevalence of AAA was estimated to be 32% in brothers. The familial AAA cases were more likely to have a ruptured AAA than the sporadic cases (8% vs. 2.4%; P AAA among brothers, support genetic contribution to AAA pathogenesis, and provide rationale for targeted screening of relatives of patients with AAA. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. Patient and Aneurysm Characteristics Predicting Prolonged Length of Stay After Elective Open AAA Repair in the Endovascular Era.

    Science.gov (United States)

    Casillas-Berumen, Sergio; Rojas-Miguez, Florencia A; Farber, Alik; Komshian, Sevan; Kalish, Jeffrey A; Rybin, Denis; Doros, Gheorghe; Siracuse, Jeffrey J

    2018-01-01

    Open aortic aneurysm repair (AAA) repair can be resource intensive and associated with a prolonged length of stay (LOS). We sought to examine patient and aneurysm predictors of prolonged LOS to better identify those at risk in the preoperative setting. Patient data were obtained from the targeted AAA American College of Surgery National Surgical Quality Improvement Program database from 2012 to 2014 of patients undergoing open AAA repair. Multivariable logistic regression was used to determine predictors of prolonged postoperative LOS defined as greater than 10 days (75th percentile). There were 1172 open AAA repairs identified. The majority (54%) of patients were older than 70 years and male (74%). Surgical approach was transperitoneal (70.9%) and retroperitoneal (29.1%). Aneurysms were 51.4% infrarenal, 33% juxtarenal, 5.7% pararenal, 7.4% suprarenal, and 2.5% type IV thoracoabdominal. Mean and median LOS were 9.1 ± 7.4 and 7 (0-72) days, respectively. Independently associated with extended LOS factors were visceral revascularization (odds ratio [OR]: 5.32, 95% confidence interval [CI]: 2.77-10.22, P AAA repair. Prospective identification of high-risk patients may allow physicians and hospitals to engage in multidisciplinary collaborations preoperatively to try to improve LOS in this resource-intensive population.

  7. NASA Astrophysics E/PO Impact: NASA SOFIA AAA Program Evaluation Results

    Science.gov (United States)

    Harman, Pamela; Backman, Dana E.; Clark, Coral; Inverness Research Sofia Aaa Evaluation Team, Wested Sofia Aaa Evaluation Team

    2015-01-01

    SOFIA is an airborne observatory, studying the universe at infrared wavelengths, capable of making observations that are impossible for even the largest and highest ground-based telescopes. SOFIA also inspires the development of new scientific instrumentation and fosters the education of young scientists and engineers.SOFIA is an 80% - 20% partnership of NASA and the German Aerospace Center (DLR), consisting of an extensively modified Boeing 747SP aircraft carrying a reflecting telescope with an effective diameter of 2.5 meters (100 inches). The SOFIA aircraft is based at NASA Armstrong Flight Research Center, Building 703, in Palmdale, California. The Science Program and Outreach Offices are located at NASA Ames Research center. SOFIA is a program in NASA's Science Mission Directorate, Astrophysics Division.Data will be collected to study many different kinds of astronomical objects and phenomena, including star cycles, solar system formation, identification of complex molecules in space, our solar system, galactic dust, nebulae and ecosystems.Airborne Astronomy Ambassador (AAA) Program:The SOFIA Education and Communications program exploits the unique attributes of airborne astronomy to contribute to national goals for the reform of science, technology, engineering, and math (STEM) education, and to elevate public scientific and technical literacy.The AAA effort is a professional development program aspiring to improve teaching, inspire students, and inform the community. To date, 55 educators from 21 states; Cycles 0, 1 and 2; have completed their astronomy professional development and their SOFIA science flight experience. Evaluation has confirmed the program's positive impact on the teacher participants, on their students, and in their communities. The inspirational experience has positively impacted their practice and career trajectory. AAAs have incorporated content knowledge and specific components of their experience into their curricula, and have given

  8. Morphological State as a Predictor for Reintervention and Mortality After EVAR for AAA

    Energy Technology Data Exchange (ETDEWEB)

    Ohrlander, Tomas [Eksjoe County Hospital (Sweden); Dencker, Magnus [Malmoe University Hospital, Department of Clinical Physiology and Nuclear Medicine (Sweden); Acosta, Stefan, E-mail: stefan.acosta@telia.com [Malmoe University Hospital, Vascular Center Malmoe-Lund (Sweden)

    2012-10-15

    Purpose: This study was designed to assess aorto-iliac morphological characteristics in relation to reintervention and all-cause long-term mortality in patients undergoing standard EVAR for infrarenal AAA. Methods: Patients treated with EVAR (Zenith{sup Registered-Sign} Stentgrafts, Cook) between May 1998 and February 2006 were prospectively enrolled in a computerized database where comorbidities and preoperative aneurysm morphology were entered. Reinterventions and mortality were checked until December 1, 2010. Median follow-up time was 68 months. Results: A total of 304 patients were included, of which 86% were men. Median age was 74 years. The reintervention rate was 23.4% (71/304). A greater diameter of the common iliac artery (p = 0.037; hazard ratio (HR) 1.037 [1.002-1.073]) was an independent factor for an increased number of reinterventions. The 30-day mortality rate was 3.0% (9/304). Aneurysm-related deaths due to AAA occurred in 4.9% (15/304). Five patients died due to a concomitant ruptured thoracic aortic aneurysm. The mortality until end of follow-up was 54.3% (165/304). The proportion of deaths caused by vascular diseases was 61.6%. The severity of angulation of the iliac arteries (p = 0.014; HR 1.018 [95% confidence interval (CI) 1.004-1.033]) and anemia (p = 0.044; HR 2.79 [95% CI 1.029-7.556]) remained as independent factors associated with all-cause long-term mortality. The crude reintervention-free survival rate at 1, 3, and 5 years was 84.5%, 64.8%, and 51.6%, respectively. Conclusions: The initial aorto-iliac morphological state in patients scheduled for standard EVAR for AAA seems to be strongly related to the need for reinterventions and long-term mortality.

  9. Selected topics in grand unification

    International Nuclear Information System (INIS)

    Seckel, D.

    1983-01-01

    This dissertation is a collection of four pieces of research dealing with grand unification. The topics are neutron oscillation, CP violation, magnetic monopole abundance and distribution in neutron stars, and a proposal for an inflationary cosmology driven by stress-energy in domain walls

  10. Introduction to grand unification theories

    International Nuclear Information System (INIS)

    Kang, Kyungsik

    1980-01-01

    We introduce the Georgi-Glashow model based on the minimal gauge group SU(5) as a prototype grand unification theory of the electroweak and strong interactions. Simple estimation of sin 2 thetasub(W) in the symmetry limit and the renormalization corrections at the energy scale of Msub(W) are given along wich other successes of the SU(5) model

  11. Coordinated Exploration for Grand Challenges

    DEFF Research Database (Denmark)

    Ørding Olsen, Anders; Sofka, Wolfgang; Grimpe, Christoph

    2016-01-01

    Grand challenges are among the most complex problems for modern societies. Many governments and foundations provide substantial resources to encourage the search for solutions. Due to the significance of these problems, organizations often form partnerships in what we call search consortia to eng...

  12. SU-E-P-16: A Feasibility Study of Using Eclipse AAA for SRS Treatement

    International Nuclear Information System (INIS)

    Lim, S; LoSasso, T

    2015-01-01

    Purpose: To commission Varian Eclipse AAA for SRS treatment and compare the accuracy with Brainlab iPlan system for clinical cases measured with radiochromic film. Methods: A 6MV AAA clinical model for a Varian TrueBeam STx is used as baseline. The focal spot and field size of the baseline model(BASE) are (1.75,0.75) and 40×40cm 2 respectively. Maximum field sizes, output factors(S t ), FWHM focal spot and secondary source sizes are systematically adjusted to obtain an optimized model(OPT) by comparing the calculated PDD’s, profiles, and output factors with measurements taken with a stereotactic diode(SD) and, cc01 and cc04 ion chambers in Blue Phantom. In-phantom dose distributions of clinical SRS fields are calculated using the OPT and the clinical Brainlab iPlan pencil-beam. Within the 90% isodose-line(ROI), the average dose difference between the calculations and radiochromic film measurements are assessed. Results: The maximum field, focal spot and secondary source sizes for the OPT are 15×15cm 2 , (0,0), and 32.3mm respectively. The OPT St input at 1.0 and 2.0cm fields are increased by 4.5% and 1.5% from BASE. The calculated output of the BASE and OPT underestimate by 16.1%–3.2% respectively at 0.5×0.5cm 2 field and 3.1%−0.02% respectively at 1.0×1.0cm 2 field. The depth doses at 10cm are within 3.5% and 0.4% of measurements for 0.5×0.5 and 1.0×1.0cm 2 . The ROI dose of OPT and iPlan are within 1.6% and 0.6% of film measurements for 3.0cm clinical fields. For 1.0cm fields, the ROI dose of OPT underestimate 0.0–2.0% and iPlan overestimates 1.7–2.9% relative to measurements. Conclusion: The small field dose calculation of Eclipse AAA algorithm can be significantly improved by carefully adjusting the input parameters. The larger deviation of the OPT for 0.5×0.5cm 2 field from measurements can be attributed to the lowest 1.0cm field size input limit of AAA. The OPT compares reasonably well with the iPlan pencil-beam and measurements

  13. SU-E-P-16: A Feasibility Study of Using Eclipse AAA for SRS Treatement

    Energy Technology Data Exchange (ETDEWEB)

    Lim, S; LoSasso, T [Memorial Sloan-Kettering Cancer Center, New York, NY (United States)

    2015-06-15

    Purpose: To commission Varian Eclipse AAA for SRS treatment and compare the accuracy with Brainlab iPlan system for clinical cases measured with radiochromic film. Methods: A 6MV AAA clinical model for a Varian TrueBeam STx is used as baseline. The focal spot and field size of the baseline model(BASE) are (1.75,0.75) and 40×40cm{sup 2} respectively. Maximum field sizes, output factors(S{sub t}), FWHM focal spot and secondary source sizes are systematically adjusted to obtain an optimized model(OPT) by comparing the calculated PDD’s, profiles, and output factors with measurements taken with a stereotactic diode(SD) and, cc01 and cc04 ion chambers in Blue Phantom. In-phantom dose distributions of clinical SRS fields are calculated using the OPT and the clinical Brainlab iPlan pencil-beam. Within the 90% isodose-line(ROI), the average dose difference between the calculations and radiochromic film measurements are assessed. Results: The maximum field, focal spot and secondary source sizes for the OPT are 15×15cm{sup 2}, (0,0), and 32.3mm respectively. The OPT St input at 1.0 and 2.0cm fields are increased by 4.5% and 1.5% from BASE. The calculated output of the BASE and OPT underestimate by 16.1%–3.2% respectively at 0.5×0.5cm{sup 2} field and 3.1%−0.02% respectively at 1.0×1.0cm{sup 2} field. The depth doses at 10cm are within 3.5% and 0.4% of measurements for 0.5×0.5 and 1.0×1.0cm{sup 2}. The ROI dose of OPT and iPlan are within 1.6% and 0.6% of film measurements for 3.0cm clinical fields. For 1.0cm fields, the ROI dose of OPT underestimate 0.0–2.0% and iPlan overestimates 1.7–2.9% relative to measurements. Conclusion: The small field dose calculation of Eclipse AAA algorithm can be significantly improved by carefully adjusting the input parameters. The larger deviation of the OPT for 0.5×0.5cm{sup 2} field from measurements can be attributed to the lowest 1.0cm field size input limit of AAA. The OPT compares reasonably well with the iPlan pencil

  14. Guidelines on CV networking information flow optimization for Texas.

    Science.gov (United States)

    2017-03-01

    Recognizing the fundamental role of information flow in future transportation applications, the research team investigated the quality and security of information flow in the connected vehicle (CV) environment. The research team identified key challe...

  15. Calibration of ADRET voltage generator type CV102. Program CODAV

    International Nuclear Information System (INIS)

    Lagarde, Gerard.

    1978-07-01

    The CODAV programm studied by the Metrology SES/SME laboratory is used for the calibration of ADRET voltage generator type CV.102. A JCAM.10 microcomputer run the measurement cycle and the printout of the results [fr

  16. Nutritive evaluation of Medicago truncutula (cv. jernalong) pasture ...

    African Journals Online (AJOL)

    Nutritive evaluation of Medicago truncutula (cv. jernalong) pasture for sheep. 1. Seasonal .... obtained by laboratory work, using in vitro techniques. (Engels et al. .... model that was used to explain 92,3% of the variance in. IVDOM content.

  17. Peak AAA Fatty Acid Homolog Contaminants Present in the Dietary Supplement l-Tryptophan Associated with the Onset of Eosinophilia-Myalgia Syndrome.

    Science.gov (United States)

    Klarskov, Klaus; Gagnon, Hugo; Racine, Mathieu; Boudreault, Pierre-Luc; Normandin, Chad; Marsault, Eric; Gleich, Gerald J; Naylor, Stephen

    2018-05-22

    The eosinophilia-myalgia syndrome (EMS) outbreak that occurred in the USA and elsewhere in 1989 was caused by the ingestion of Showa Denko K.K. (SD) L-tryptophan (L-Trp). "Six compounds" detected in the L-Trp were reported as case-associated contaminants. Recently the final and most statistically significant contaminant, "Peak AAA" was structurally characterized. The "compound" was actually shown to be two structural isomers resulting from condensation reactions of L-Trp with fatty acids derived from the bacterial cell membrane. They were identified as the indole C-2 anteiso (AAA 1 -343) and linear (AAA 2 -343) aliphatic chain isomers. Based on those findings, we utilized a combination of on-line HPLC-electrospray ionization mass spectrometry (LC-MS), as well as both precursor and product ion tandem mass spectrometry (MS/MS) to facilitate identification of a homologous family of condensation products related to AAA 1 -343 and AAA 2 -343. We structurally characterized eight new AAA 1 -XXX/AAA 2 -XXX contaminants, where XXX represents the integer molecular ions of all the related homologs, differing by aliphatic chain length and isomer configuration. The contaminants were derived from the following fatty acids of the bacterial cell membrane, 5-methylheptanoic acid (anteiso-C8:0) for AAA 1 -315; n-octanoic acid (n-C8:0) for AAA 2 -315; 6-methyloctanoic acid (anteiso-C9:0) for AAA 1 -329; n-nonanoic acid (n-C9:0) for AAA 2 -329; 10-methyldodecanoic acid (anteiso-C13:0) for AAA 1 -385; n-tridecanoic acid (n-C13:0) for AAA 2 -385; 11-methyltridecanoic acid (anteiso-C14:0) for AAA 1 -399; and n-tetradecanoic acid (n-C14:0) for AAA 2 -399. The concentration levels for these contaminants were estimated to be 0.1 - 7.9 μg / 500 mg of an individual SD L-Trp tablet or capsule The structural similarity of these homologs to case-related contaminants of Spanish Toxic Oil Syndrome (TOS) is discussed. Copyright © 2018. Published by Elsevier B.V.

  18. Increased galectin-3 levels are associated with abdominal aortic aneurysm progression and inhibition of galectin-3 decreases elastase-induced AAA development.

    Science.gov (United States)

    Fernandez-García, Carlos-Ernesto; Tarin, Carlos; Roldan-Montero, Raquel; Martinez-Lopez, Diego; Torres-Fonseca, Monica; Lindhot, Jes S; Vega de Ceniga, Melina; Egido, Jesus; Lopez-Andres, Natalia; Blanco-Colio, Luis-Miguel; Martín-Ventura, Jose-Luis

    2017-11-15

    Abdominal aortic aneurysm (AAA) evolution is unpredictable and no specific treatment exists for AAA, except surgery to prevent aortic rupture. Galectin-3 has been previously associated with CVD, but its potential role in AAA has not been addressed. Galectin-3 levels were increased in the plasma of AAA patients ( n =225) compared with the control group ( n =100). In addition, galectin-3 concentrations were associated with the need for surgical repair, independently of potential confounding factors. Galectin-3 mRNA and protein expression were increased in human AAA samples compared with healthy aortas. Experimental AAA in mice was induced via aortic elastase perfusion. Mice were treated intravenously with the galectin-3 inhibitor modified citrus pectin (MCP, 10 mg/kg, every other day) or saline. Similar to humans, galectin-3 serum and aortic mRNA levels were also increased in elastase-induced AAA mice compared with control mice. Mice treated with MCP showed decreased aortic dilation, as well as elastin degradation, vascular smooth muscle cell (VSMC) loss, and macrophage content at day 14 postelastase perfusion compared with control mice. The underlying mechanism(s) of the protective effect of MCP was associated with a decrease in galectin-3 and cytokine (mainly CCL5) mRNA and protein expression. Interestingly, galectin-3 induced CCL5 expression by a mechanism involving STAT3 activation in VSMC. Accordingly, MCP treatment decreased STAT3 phosphorylation in elastase-induced AAA. In conclusion, increased galectin-3 levels are associated with AAA progression, while galectin-3 inhibition decreased experimental AAA development. Our data suggest the potential role of galectin-3 as a therapeutic target in AAA. © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

  19. Utilización del método de superficie de respuesta para formular una base de banano (Musa AAA para batidos

    Directory of Open Access Journals (Sweden)

    Marta Gamboa White

    2010-06-01

    Full Text Available El Método de Superficie de Respuesta se utiliza para optimizar o reformular productos. Se usa principalmente para economizar dinero y reducir el tiempo de pruebas, al disminuir el número de ensayos. Se presenta la aplicación del Método de Superficie de Respuesta para desarrollar una base a partir de bananos (Musa AAA var. Cavendish cv. Gran Enano de rechazo de exportación a manera de caso para estudiar su uso. Por medio de una encuesta se identificó que el dulzor, espumosidad y sabor fueron los atributos que los consumidores consideraron más importantes en un batido. Con base en estos atributos y con los ingredientes: pulpa de banano, goma guar y azúcar, se elaboraron y seleccionaron varias mezclas base, que fueron degustadas, en forma de batidos en leche, por un grupo de 90 consumidores. Por medio del diseño de superficie de respuesta se determinó que el batido con la mayor aceptación estuvo constituido por un 77,0 % de leche y un 23,0 % de mezcla base, compuesta esta última por 0,12 % de goma guar, 80,50 % de pulpa de banano y un 19,50 % de azúcar. Un Análisis de Componentes Principales permitió determinar que el contenido de sacarosa, que define el grado de dulzor, fue el atributo más importante para la aceptación del batido y se demostró que la combinación del Método de Superficie de Respuesta con el Análisis de Componentes Principales constituyó una herramienta útil en la formulación y optimización de productos, sobre todo para entender la interrelación de las variables.

  20. The ATPase of the phi29 DNA packaging motor is a member of the hexameric AAA+ superfamily.

    Science.gov (United States)

    Schwartz, Chad; De Donatis, Gian Marco; Fang, Huaming; Guo, Peixuan

    2013-08-15

    The AAA+ superfamily of proteins is a class of motor ATPases performing a wide range of functions that typically exist as hexamers. The ATPase of phi29 DNA packaging motor has long been a subject of debate in terms of stoichiometry and mechanism of action. Here, we confirmed the stoichiometry of phi29 motor ATPase to be a hexamer and provide data suggesting that the phi29 motor ATPase is a member of the classical hexameric AAA+ superfamily. Native PAGE, EMSA, capillary electrophoresis, ATP titration, and binomial distribution assay show that the ATPase is a hexamer. Mutations in the known Walker motifs of the ATPase validated our previous assumptions that the protein exists as another member of this AAA+ superfamily. Our data also supports the finding that the phi29 DNA packaging motor uses a revolution mechanism without rotation or coiling (Schwartz et al., this issue). Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

  1. Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state

    Science.gov (United States)

    Morito, Daisuke; Nishikawa, Kouki; Hoseki, Jun; Kitamura, Akira; Kotani, Yuri; Kiso, Kazumi; Kinjo, Masataka; Fujiyoshi, Yoshinori; Nagata, Kazuhiro

    2014-03-01

    Moyamoya disease is an idiopathic human cerebrovascular disorder that is characterized by progressive stenosis and abnormal collateral vessels. We recently identified mysterin/RNF213 as its first susceptibility gene, which encodes a 591-kDa protein containing enzymatically active P-loop ATPase and ubiquitin ligase domains and is involved in proper vascular development in zebrafish. Here we demonstrate that mysterin further contains two tandem AAA+ ATPase modules and forms huge ring-shaped oligomeric complex. AAA+ ATPases are known to generally mediate various biophysical and mechanical processes with the characteristic ring-shaped structure. Fluorescence correlation spectroscopy and biochemical evaluation suggested that mysterin dynamically changes its oligomeric forms through ATP/ADP binding and hydrolysis cycles. Thus, the moyamoya disease-associated gene product is a unique protein that functions as ubiquitin ligase and AAA+ ATPase, which possibly contributes to vascular development through mechanical processes in the cell.

  2. Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state

    Science.gov (United States)

    Morito, Daisuke; Nishikawa, Kouki; Hoseki, Jun; Kitamura, Akira; Kotani, Yuri; Kiso, Kazumi; Kinjo, Masataka; Fujiyoshi, Yoshinori; Nagata, Kazuhiro

    2014-01-01

    Moyamoya disease is an idiopathic human cerebrovascular disorder that is characterized by progressive stenosis and abnormal collateral vessels. We recently identified mysterin/RNF213 as its first susceptibility gene, which encodes a 591-kDa protein containing enzymatically active P-loop ATPase and ubiquitin ligase domains and is involved in proper vascular development in zebrafish. Here we demonstrate that mysterin further contains two tandem AAA+ ATPase modules and forms huge ring-shaped oligomeric complex. AAA+ ATPases are known to generally mediate various biophysical and mechanical processes with the characteristic ring-shaped structure. Fluorescence correlation spectroscopy and biochemical evaluation suggested that mysterin dynamically changes its oligomeric forms through ATP/ADP binding and hydrolysis cycles. Thus, the moyamoya disease-associated gene product is a unique protein that functions as ubiquitin ligase and AAA+ ATPase, which possibly contributes to vascular development through mechanical processes in the cell. PMID:24658080

  3. Science | AAAS

    Science.gov (United States)

    Giuseppe Conte Italy's new prime minister defended discredited stem cell therapy By Luca Tancredi Barone defended discredited stem cell therapy By Luca Tancredi Barone May. 24, 2018 illustration of GOES-17 | May. 24, 2018 STEPHAN JUNEK/MPI BRAIN RESEARCH Apparatus for building a single NaCs molecule from two

  4. Editor's Choice - Prolonged ICU Length of Stay after AAA Repair: Analysis of Time Trends and Long-term Outcome.

    Science.gov (United States)

    Gavali, H; Mani, K; Tegler, G; Kawati, R; Covaciu, L; Wanhainen, A

    2017-08-01

    The aim of the study was to investigate the frequency and outcome of prolonged intensive care unit (ICU) length of stay (LOS) after abdominal aortic aneurysm (AAA) repair in the endovascular era. All patients operated on for AAA between 1999 and 2013 at Uppsala University hospital were identified. Data were retrieved from the Swedish Vascular registry, the Swedish Intensive Care registry, the National Population registry, and case records. Prolonged ICU LOS was defined as ≥ 48 h during the primary hospital stay. Patients surviving ≥ 48 h after AAA surgery were included in the analysis. A total of 725 patients were identified, of whom 707 (97.5%) survived ≥ 48 h; 563 (79.6%) underwent intact AAA repair and 144 (20.4%) ruptured AAA repair. A total of 548 patients (77.5%) required AAA repairs in 1999 to 7.3% in 2013 (p < .001) whereas the use of endovascular aortic repair (EVAR) increased from 6.9% in 1999 to 78.0% in 2013 (p < .001). The 30 day survival rate was 98.2% for those with < 48 h ICU stay versus 93.0% for 2-6 days versus 81.8% for ≥ 7 days (p < .001); the corresponding 90 day survival was 97.1% versus 86.1% versus 63.6% (p < .001) respectively. For patients surviving 90 days after repair, there was no difference in long-term survival between the groups. During the period of progressively increasing use of EVAR, a simultaneous significant reduction in frequency of prolonged ICU LOS occurred. Although prolonged ICU LOS was associated with a high short-term mortality, long-term outcome among those surviving the initial 90 days was less affected. Copyright © 2017 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.

  5. Structure determination of disease associated peak AAA from l-Tryptophan implicated in the eosinophilia-myalgia syndrome.

    Science.gov (United States)

    Klarskov, Klaus; Gagnon, Hugo; Boudreault, Pierre-Luc; Normandin, Chad; Plancq, Baptiste; Marsault, Eric; Gleich, Gerald J; Naylor, Stephen

    2018-01-05

    The eosinophilia-myalgia syndrome (EMS) outbreak of 1989 that occurred in the USA and elsewhere was caused by the ingestion of l-Tryptophan (L-Trp) solely manufactured by the Japanese company Showa Denko K.K. (SD). Six compounds present in the SD L-Trp were reported to be case-associated contaminants. However, "one" of these compounds, Peak AAA has remained structurally uncharacterized, despite the fact that it was described as "the only statistically significant (p=0.0014) contaminant". Here, we employ on-line microcapillary-high performance liquid chromatography-electrospray ionization mass spectrometry (LC-MS), and tandem mass spectrometry (MS/MS) to determine that Peak AAA is in fact two structurally related isomers. Peak AAA 1 and Peak AAA 2 differed in LC retention times, and were determined by accurate mass-LC-MS to both have a protonated molecular ion (MH +) of mass 343.239Da (Da), corresponding to a molecular formula of C 21 H 30 N 2 O 2, and possessing eight degrees of unsaturation (DoU) for the non-protonated molecule. By comparing the LC-MS and LC-MS-MS retention times and spectra with authentic synthetic standards, Peak AAA 1 was identified as the intermolecular condensation product of L-Trp with anteiso 7-methylnonanoic acid, to afford (S)-2-amino-3-(2-((S,E)-7-methylnon-1-en-1-yl)-1H-indol-3-yl)propanoic acid. Peak AAA 2 was determined to be a condensation product of L-Trp with decanoic acid, which produced (S)-2-amino-3-(2-((E)-dec-1-en-1-yl)-1H-indol-3-yl)propanoic acid. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. The Abdominal Aortic Aneurysm Statistically Corrected Operative Risk Evaluation (AAA SCORE) for predicting mortality after open and endovascular interventions.

    Science.gov (United States)

    Ambler, Graeme K; Gohel, Manjit S; Mitchell, David C; Loftus, Ian M; Boyle, Jonathan R

    2015-01-01

    Accurate adjustment of surgical outcome data for risk is vital in an era of surgeon-level reporting. Current risk prediction models for abdominal aortic aneurysm (AAA) repair are suboptimal. We aimed to develop a reliable risk model for in-hospital mortality after intervention for AAA, using rigorous contemporary statistical techniques to handle missing data. Using data collected during a 15-month period in the United Kingdom National Vascular Database, we applied multiple imputation methodology together with stepwise model selection to generate preoperative and perioperative models of in-hospital mortality after AAA repair, using two thirds of the available data. Model performance was then assessed on the remaining third of the data by receiver operating characteristic curve analysis and compared with existing risk prediction models. Model calibration was assessed by Hosmer-Lemeshow analysis. A total of 8088 AAA repair operations were recorded in the National Vascular Database during the study period, of which 5870 (72.6%) were elective procedures. Both preoperative and perioperative models showed excellent discrimination, with areas under the receiver operating characteristic curve of .89 and .92, respectively. This was significantly better than any of the existing models (area under the receiver operating characteristic curve for best comparator model, .84 and .88; P AAA repair. These models were carefully developed with rigorous statistical methodology and significantly outperform existing methods for both elective cases and overall AAA mortality. These models will be invaluable for both preoperative patient counseling and accurate risk adjustment of published outcome data. Copyright © 2015 Society for Vascular Surgery. Published by Elsevier Inc. All rights reserved.

  7. Structural dynamics of the MecA-ClpC complex: a type II AAA+ protein unfolding machine.

    Science.gov (United States)

    Liu, Jing; Mei, Ziqing; Li, Ningning; Qi, Yutao; Xu, Yanji; Shi, Yigong; Wang, Feng; Lei, Jianlin; Gao, Ning

    2013-06-14

    The MecA-ClpC complex is a bacterial type II AAA(+) molecular machine responsible for regulated unfolding of substrates, such as transcription factors ComK and ComS, and targeting them to ClpP for degradation. The six subunits of the MecA-ClpC complex form a closed barrel-like structure, featured with three stacked rings and a hollow passage, where substrates are threaded and translocated through successive pores. Although the general concepts of how polypeptides are unfolded and translocated by internal pore loops of AAA(+) proteins have long been conceived, the detailed mechanistic model remains elusive. With cryoelectron microscopy, we captured four different structures of the MecA-ClpC complexes. These complexes differ in the nucleotide binding states of the two AAA(+) rings and therefore might presumably reflect distinctive, representative snapshots from a dynamic unfolding cycle of this hexameric complex. Structural analysis reveals that nucleotide binding and hydrolysis modulate the hexameric complex in a number of ways, including the opening of the N-terminal ring, the axial and radial positions of pore loops, the compactness of the C-terminal ring, as well as the relative rotation between the two nucleotide-binding domain rings. More importantly, our structural and biochemical data indicate there is an active allosteric communication between the two AAA(+) rings and suggest that concerted actions of the two AAA(+) rings are required for the efficiency of the substrate unfolding and translocation. These findings provide important mechanistic insights into the dynamic cycle of the MecA-ClpC unfoldase and especially lay a foundation toward the complete understanding of the structural dynamics of the general type II AAA(+) hexamers.

  8. TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching.

    Science.gov (United States)

    Ye, Qiaozhen; Rosenberg, Scott C; Moeller, Arne; Speir, Jeffrey A; Su, Tiffany Y; Corbett, Kevin D

    2015-04-28

    The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family. Here we present the structure of the Caenorhabditis elegans TRIP13 ortholog PCH-2, revealing a new family of AAA+ ATPase protein remodelers. PCH-2 possesses a substrate-recognition domain related to those of the protein remodelers NSF and p97, while its overall hexameric architecture and likely structural mechanism bear close similarities to the bacterial protein unfoldase ClpX. We find that TRIP13, aided by the adapter protein p31(comet), converts the HORMA-family spindle checkpoint protein MAD2 from a signaling-active 'closed' conformer to an inactive 'open' conformer. We propose that TRIP13 and p31(comet) collaborate to inactivate the spindle assembly checkpoint through MAD2 conformational conversion and disassembly of mitotic checkpoint complexes. A parallel HORMA protein disassembly activity likely underlies TRIP13's critical regulatory functions in meiotic chromosome structure and recombination.

  9. TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching

    Energy Technology Data Exchange (ETDEWEB)

    Ye, Qiaozhen [Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, United States; Rosenberg, Scott C. [Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, United States; Moeller, Arne [National Resource for Automated Molecular Microscopy, Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, United States; Speir, Jeffrey A. [National Resource for Automated Molecular Microscopy, Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, United States; Su, Tiffany Y. [Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, United States; Corbett, Kevin D. [Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, United States; Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States

    2015-04-28

    The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family. Here we present the structure of the Caenorhabditis elegans TRIP13 ortholog PCH-2, revealing a new family of AAA+ ATPase protein remodelers. PCH-2 possesses a substrate-recognition domain related to those of the protein remodelers NSF and p97, while its overall hexameric architecture and likely structural mechanism bear close similarities to the bacterial protein unfoldase ClpX. We find that TRIP13, aided by the adapter protein p31(comet), converts the HORMA-family spindle checkpoint protein MAD2 from a signaling-active ‘closed’ conformer to an inactive ‘open’ conformer. We propose that TRIP13 and p31(comet) collaborate to inactivate the spindle assembly checkpoint through MAD2 conformational conversion and disassembly of mitotic checkpoint complexes. A parallel HORMA protein disassembly activity likely underlies TRIP13's critical regulatory functions in meiotic chromosome structure and recombination.

  10. Inflammatory cell phenotypes in AAAs; their role and potential as targets for therapy

    Science.gov (United States)

    Dale, Matthew A; Ruhlman, Melissa K.; Baxter, B. Timothy

    2015-01-01

    Abdominal aortic aneurysms are characterized by chronic inflammatory cell infiltration. AAA is typically an asymptomatic disease and caused approximately 15,000 deaths annually in the U.S. Previous studies have examined both human and murine aortic tissue for the presence of various inflammatory cell types. Studies show that in both human and experimental AAAs, prominent inflammatory cell infiltration, such as CD4+ T cells and macrophages, occurs in the damaged aortic wall. These cells have the ability to undergo phenotypic modulation based on microenvironmental cues, potentially influencing disease progression. Pro-inflammatory CD4+ T cells and classically activated macrophages dominate the landscape of aortic infiltrates. The skew to pro-inflammatory phenotypes alters disease progression and plays a role in causing chronic inflammation. The local cytokine production and presence of inflammatory mediators, such as extracellular matrix breakdown products, influence the uneven balance of the inflammatory infiltrate phenotypes. Understanding and developing new strategies that target the pro-inflammatory phenotype could provide useful therapeutic targets for a disease with no current pharmacological intervention. PMID:26044582

  11. Crystal Structure and Biochemical Characterization of a Mycobacterium smegmatis AAA-Type Nucleoside Triphosphatase Phosphohydrolase (Msm0858).

    Science.gov (United States)

    Unciuleac, Mihaela-Carmen; Smith, Paul C; Shuman, Stewart

    2016-05-15

    AAA proteins (ATPases associated with various cellular activities) use the energy of ATP hydrolysis to drive conformational changes in diverse macromolecular targets. Here, we report the biochemical characterization and 2.5-Å crystal structure of a Mycobacterium smegmatis AAA protein Msm0858, the ortholog of Mycobacterium tuberculosis Rv0435c. Msm0858 is a magnesium-dependent ATPase and is active with all nucleoside triphosphates (NTPs) and deoxynucleoside triphosphates (dNTPs) as substrates. The Msm0858 structure comprises (i) an N-terminal domain (amino acids [aa] 17 to 201) composed of two β-barrel modules and (ii) two AAA domains, D1 (aa 212 to 473) and D2 (aa 476 to 744), each of which has ADP in the active site. Msm0858-ADP is a monomer in solution and in crystallized form. Msm0858 domains are structurally homologous to the corresponding modules of mammalian p97. However, the position of the N-domain modules relative to the AAA domains in the Msm0858-ADP tertiary structure is different and would impede the formation of a p97-like hexameric quaternary structure. Mutational analysis of the A-box and B-box motifs indicated that the D1 and D2 AAA domains are both capable of ATP hydrolysis. Simultaneous mutations of the D1 and D2 active-site motifs were required to abolish ATPase activity. ATPase activity was effaced by mutation of the putative D2 arginine finger, suggesting that Msm0858 might oligomerize during the ATPase reaction cycle. A truncated variant Msm0858 (aa 212 to 745) that lacks the N domain was characterized as a catalytically active homodimer. Recent studies have underscored the importance of AAA proteins (ATPases associated with various cellular activities) in the physiology of mycobacteria. This study reports the ATPase activity and crystal structure of a previously uncharacterized mycobacterial AAA protein, Msm0858. Msm0858 consists of an N-terminal β-barrel domain and two AAA domains, each with ADP bound in the active site. Msm0858 is a

  12. Analysis of positional candidate genes in the AAA1 susceptibility locus for abdominal aortic aneurysms on chromosome 19

    Directory of Open Access Journals (Sweden)

    Ferrell Robert E

    2011-01-01

    Full Text Available Abstract Background Abdominal aortic aneurysm (AAA is a complex disorder with multiple genetic risk factors. Using affected relative pair linkage analysis, we previously identified an AAA susceptibility locus on chromosome 19q13. This locus has been designated as the AAA1 susceptibility locus in the Online Mendelian Inheritance in Man (OMIM database. Methods Nine candidate genes were selected from the AAA1 locus based on their function, as well as mRNA expression levels in the aorta. A sample of 394 cases and 419 controls was genotyped for 41 SNPs located in or around the selected nine candidate genes using the Illumina GoldenGate platform. Single marker and haplotype analyses were performed. Three genes (CEBPG, PEPD and CD22 were selected for DNA sequencing based on the association study results, and exonic regions were analyzed. Immunohistochemical staining of aortic tissue sections from AAA and control individuals was carried out for the CD22 and PEPD proteins with specific antibodies. Results Several SNPs were nominally associated with AAA (p CEBPG, peptidase D (PEPD, and CD22. Haplotype analysis found a nominally associated 5-SNP haplotype in the CEBPG/PEPD locus, as well as a nominally associated 2-SNP haplotype in the CD22 locus. DNA sequencing of the coding regions revealed no variation in CEBPG. Seven sequence variants were identified in PEPD, including three not present in the NCBI SNP (dbSNP database. Sequencing of all 14 exons of CD22 identified 20 sequence variants, five of which were in the coding region and six were in the 3'-untranslated region. Five variants were not present in dbSNP. Immunohistochemical staining for CD22 revealed protein expression in lymphocytes present in the aneurysmal aortic wall only and no detectable expression in control aorta. PEPD protein was expressed in fibroblasts and myofibroblasts in the media-adventitia border in both aneurysmal and non-aneurysmal tissue samples. Conclusions Association testing

  13. Introducing AAA-MS, a rapid and sensitive method for amino acid analysis using isotope dilution and high-resolution mass spectrometry.

    Science.gov (United States)

    Louwagie, Mathilde; Kieffer-Jaquinod, Sylvie; Dupierris, Véronique; Couté, Yohann; Bruley, Christophe; Garin, Jérôme; Dupuis, Alain; Jaquinod, Michel; Brun, Virginie

    2012-07-06

    Accurate quantification of pure peptides and proteins is essential for biotechnology, clinical chemistry, proteomics, and systems biology. The reference method to quantify peptides and proteins is amino acid analysis (AAA). This consists of an acidic hydrolysis followed by chromatographic separation and spectrophotometric detection of amino acids. Although widely used, this method displays some limitations, in particular the need for large amounts of starting material. Driven by the need to quantify isotope-dilution standards used for absolute quantitative proteomics, particularly stable isotope-labeled (SIL) peptides and PSAQ proteins, we developed a new AAA assay (AAA-MS). This method requires neither derivatization nor chromatographic separation of amino acids. It is based on rapid microwave-assisted acidic hydrolysis followed by high-resolution mass spectrometry analysis of amino acids. Quantification is performed by comparing MS signals from labeled amino acids (SIL peptide- and PSAQ-derived) with those of unlabeled amino acids originating from co-hydrolyzed NIST standard reference materials. For both SIL peptides and PSAQ standards, AAA-MS quantification results were consistent with classical AAA measurements. Compared to AAA assay, AAA-MS was much faster and was 100-fold more sensitive for peptide and protein quantification. Finally, thanks to the development of a labeled protein standard, we also extended AAA-MS analysis to the quantification of unlabeled proteins.

  14. Results from KASCADE–Grande

    International Nuclear Information System (INIS)

    Bertaina, M.; Apel, W.D.; Arteaga-Velázquez, J.C.; Bekk, K.; Blümer, J.; Bozdog, H.; Brancus, I.M.; Buchholz, P.; Cantoni, E.; Chiavassa, A.; Cossavella, F.

    2012-01-01

    The KASCADE–Grande experiment, located at Karlsruhe Institute of Technology (Germany) is a multi-component extensive air-shower experiment devoted to the study of cosmic rays and their interactions at primary energies 10 14 –10 18 eV. Main goals of the experiment are the measurement of the all-particle energy spectrum and mass composition in the 10 16 –10 18 eV range by sampling charged (N ch ) and muon (N μ ) components of the air shower. The method to derive the energy spectrum and its uncertainties, as well as the implications of the obtained result, is discussed. An overview of the analyses performed by KASCADE–Grande to derive the mass composition of the measured high-energy comic rays is presented as well.

  15. Results from KASCADE-Grande

    Energy Technology Data Exchange (ETDEWEB)

    Bertaina, M., E-mail: bertaina@to.infn.it [Dipartimento di Fisica Generale dell' Universita, Torino (Italy); Apel, W.D. [Institut fuer Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); Arteaga-Velazquez, J.C. [Universidad Michoacana, Instituto de Fisica y Matematicas, Morelia (Mexico); Bekk, K. [Institut fuer Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); Bluemer, J. [Institut fuer Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); Institut fuer Experimentelle Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); Bozdog, H. [Institut fuer Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); Brancus, I.M. [National Institute of Physics and Nuclear Engineering, Bucharest (Romania); Buchholz, P. [Fachbereich Physik, Universitaet Siegen (Germany); Cantoni, E. [Dipartimento di Fisica Generale dell' Universita, Torino (Italy); Istituto di Fisica dello Spazio Interplanetario, INAF Torino (Italy); Chiavassa, A. [Dipartimento di Fisica Generale dell' Universita, Torino (Italy); Cossavella, F. [Institut fuer Experimentelle Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); and others

    2012-11-11

    The KASCADE-Grande experiment, located at Karlsruhe Institute of Technology (Germany) is a multi-component extensive air-shower experiment devoted to the study of cosmic rays and their interactions at primary energies 10{sup 14}-10{sup 18} eV. Main goals of the experiment are the measurement of the all-particle energy spectrum and mass composition in the 10{sup 16}-10{sup 18} eV range by sampling charged (N{sub ch}) and muon (N{sub {mu}}) components of the air shower. The method to derive the energy spectrum and its uncertainties, as well as the implications of the obtained result, is discussed. An overview of the analyses performed by KASCADE-Grande to derive the mass composition of the measured high-energy comic rays is presented as well.

  16. Cassini's Grand Finale Science Highlights

    Science.gov (United States)

    Spilker, Linda

    2017-10-01

    After 13 years in orbit, the Cassini-Huygens Mission to Saturn ended in a science-rich blaze of glory. Cassini returned its final bits of unique science data on September 15, 2017, as it plunged into Saturn's atmosphere satisfying planetary protection requirements. Cassini's Grand Finale covered a period of roughly five months and ended with the first time exploration of the region between the rings and planet.The final close flyby of Titan in late April 2017 propelled Cassini across Saturn’s main rings and into its Grand Finale orbits; 22 orbits that repeatedly dove between Saturn’s innermost rings and upper atmosphere making Cassini the first spacecraft to explore this region. The last orbit turned the spacecraft into the first Saturn upper atmospheric probe.The Grand Finale orbits provided highest resolution observations of both the rings and Saturn, and in-situ sampling of the ring particle composition, Saturn's atmosphere, plasma, and innermost radiation belts. The gravitational field was measured to unprecedented accuracy, providing information on the interior structure of the planet, winds in the deeper atmosphere, and mass of the rings. The magnetic field provided insight into the physical nature of the magnetic dynamo and structure of the internal magnetic field. The ion and neutral mass spectrometer sampled the upper atmosphere for molecules that escape the atmosphere in addition to molecules originating from the rings. The cosmic dust analyzer directly sampled the composition from different parts of the main rings for the first time. Fields and particles instruments directly measured the plasma environment between the rings and planet.Science highlights and new mysteries gleaned to date from the Grand Finale orbits will be discussed.The research described in this paper was carried out in part at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration. Copyright 2017

  17. Grandes números primos

    OpenAIRE

    Bang, Thöger

    2012-01-01

    En las escuelas danesas la teoría de los números se ha ido restringiendo gradualmente hasta no incluir sino la demostración de la descomposición univoca de los números enteros en números primos y la demostración del teorema clásico de EUCLIDES sobre la existencia de un número primo arbitrariamente grande.

  18. A Non-Competitive Inhibitor of VCP/p97 and VPS4 Reveals Conserved Allosteric Circuits in Type I and II AAA ATPases.

    Science.gov (United States)

    Pöhler, Robert; Krahn, Jan H; van den Boom, Johannes; Dobrynin, Grzegorz; Kaschani, Farnusch; Eggenweiler, Hans-Michael; Zenke, Frank T; Kaiser, Markus; Meyer, Hemmo

    2018-02-05

    AAA ATPases have pivotal functions in diverse cellular processes essential for survival and proliferation. Revealing strategies for chemical inhibition of this class of enzymes is therefore of great interest for the development of novel chemotherapies or chemical tools. Here, we characterize the compound MSC1094308 as a reversible, allosteric inhibitor of the type II AAA ATPase human ubiquitin-directed unfoldase (VCP)/p97 and the type I AAA ATPase VPS4B. Subsequent proteomic, genetic and biochemical studies indicate that MSC1094308 binds to a previously characterized drugable hotspot of p97, thereby inhibiting the D2 ATPase activity. Our results furthermore indicate that a similar allosteric site exists in VPS4B, suggesting conserved allosteric circuits and drugable sites in both type I and II AAA ATPases. Our results may thus guide future chemical tool and drug discovery efforts for the biomedically relevant AAA ATPases. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. Grand unification theory and technicolor

    International Nuclear Information System (INIS)

    Rubakov, V.A.; Shaposhnikov, M.E.

    1983-01-01

    The lecture course can be considered as introduction to the problems concerning grand unification models. The course is incomplete. Such problems as CP-violations in strong interactions and the problem of gravitational interaction inclusion in the scheme of grand unification theory are not touched upon. Models of early unification, in which strong, weak and electromagnetic interactions are compared according to the ''strength'' at energies of about 10 5 -10 6 GeV, are not discussed. Models with horizontal symmetry, considering different generations of quarks and leptons from one viewpoint, are not analyzed. Cosmological applications of supersymmetric unified theories are not considered. Certain problems of standard elementary particle theory, philosophy of the great unification, general properties of the grand unification models and the main principles of the construction of models: the SU(5) model, models on the SO(10) groups, have been considered. The problem of supersymmetric unification hierarchies, supersymmetric generalization of the minimum SU(5) model, supersymmetry violation and the problem of hierarchies, phenomenology of the o.rand unification models, cosmological application and technicolour, are discussed

  20. Monte Carlo evaluation of the AAA treatment planning algorithm in a heterogeneous multilayer phantom and IMRT clinical treatments for an Elekta SL25 linear accelerator

    International Nuclear Information System (INIS)

    Sterpin, E.; Tomsej, M.; Smedt, B. de; Reynaert, N.; Vynckier, S.

    2007-01-01

    The Anisotropic Analytical Algorithm (AAA) is a new pencil beam convolution/superposition algorithm proposed by Varian for photon dose calculations. The configuration of AAA depends on linear accelerator design and specifications. The purpose of this study was to investigate the accuracy of AAA for an Elekta SL25 linear accelerator for small fields and intensity modulated radiation therapy (IMRT) treatments in inhomogeneous media. The accuracy of AAA was evaluated in two studies. First, AAA was compared both with Monte Carlo (MC) and the measurements in an inhomogeneous phantom simulating lung equivalent tissues and bone ribs. The algorithm was tested under lateral electronic disequilibrium conditions, using small fields (2x2 cm 2 ). Good agreement was generally achieved for depth dose and profiles, with deviations generally below 3% in lung inhomogeneities and below 5% at interfaces. However, the effects of attenuation and scattering close to the bone ribs were not fully taken into account by AAA, and small inhomogeneities may lead to planning errors. Second, AAA and MC were compared for IMRT plans in clinical conditions, i.e., dose calculations in a computed tomography scan of a patient. One ethmoid tumor, one orophaxynx and two lung tumors are presented in this paper. Small differences were found between the dose volume histograms. For instance, a 1.7% difference for the mean planning target volume dose was obtained for the ethmoid case. Since better agreement was achieved for the same plans but in homogeneous conditions, these differences must be attributed to the handling of inhomogeneities by AAA. Therefore, inherent assumptions of the algorithm, principally the assumption of independent depth and lateral directions in the scaling of the kernels, were slightly influencing AAA's validity in inhomogeneities. However, AAA showed a good accuracy overall and a great ability to handle small fields in inhomogeneous media compared to other pencil beam convolution

  1. Prevalence of previously undiagnosed abdominal aortic aneurysms in the area of Como: the ComoCuore "looking for AAA" ultrasonography screening.

    Science.gov (United States)

    Corrado, Giovanni; Durante, Alessandro; Genchi, Vincenzo; Trabattoni, Loris; Beretta, Sandro; Rovelli, Enza; Foglia-Manzillo, Giovanni; Ferrari, Giovanni

    2016-08-01

    The prognosis for abdominal aortic aneurysm (AAA) rupture is poor. Long-term follow-up of population-based randomized trials has demonstrated that ultrasound (US) screening for abdominal aortic aneurysms (AAAs) measuring 3 cm or greater decreases AAA-related mortality rates and is cost-effective. We though to prospectively perform during a 26-month period a limited US examination of the infrarenal aorta in volunteers of both gender aged 60-85 years without history of AAA living in the area of Como, Italy. From September 2010 to November 2013 ComoCuore, a no-profit nongovernmental association, enrolled 1555 people (aged 68.8 ± 6.8 years; 48.6 % males). Clinical data and a US imaging of the aorta were collected for each participant. AAA was found in 22 volunteers (1.4 %) mainly males (2.5 % in males vs. 0.4 % in females p = 0.005). Overall, the prevalence of cardiovascular risk factors was higher in patients with vs. without AAA (mean 2.9 ± 3.0 vs. 1.4 ± 1.0 respectively, p AAA on multivariate analysis were age (OR 1.14, 1.06-1.22; p AAA in a large cohort of asymptomatic people. Independent predictors of AAA were male sex, older age and a history of smoking. Accordingly to recent data the prevalence of AAA seems to be declining, maybe due to a reduction of smoking in Italy.

  2. Foot-and-Mouth Disease Virus 2C Is a Hexameric AAA+ Protein with a Coordinated ATP Hydrolysis Mechanism

    DEFF Research Database (Denmark)

    Sweeney, Trevor; Cisnetto, Valentina; Bose, Daniel

    2010-01-01

    Foot-and-mouth disease virus (FMDV), a positive sense, single-stranded RNA virus, causes a highly contagious disease in cloven-hoofed livestock. Like other picornaviruses, FMDV has a conserved 2C protein assigned to the superfamily 3 helicases a group of AAA+ ATPases that has a predicted N-termin...

  3. A conserved inter-domain communication mechanism regulates the ATPase activity of the AAA-protein Drg1

    NARCIS (Netherlands)

    Prattes, M.; Loibl, M.; Zisser, G.; Luschnig, D.; Kappel, L.; Rossler, I.; Grassegger, M.; Hromic, A.; Krieger, E.; Gruber, K.; Pertschy, B.; Bergler, H.

    2017-01-01

    AAA-ATPases fulfil essential roles in different cellular pathways and often act in form of hexameric complexes. Interaction with pathway-specific substrate and adaptor proteins recruits them to their targets and modulates their catalytic activity. This substrate dependent regulation of ATP

  4. Deviation of the typical AAA substrate-threading pore prevents fatal protein degradation in yeast Cdc48.

    Science.gov (United States)

    Esaki, Masatoshi; Islam, Md Tanvir; Tani, Naoki; Ogura, Teru

    2017-07-14

    Yeast Cdc48 is a well-conserved, essential chaperone of ATPases associated with diverse cellular activity (AAA) proteins, which recognizes substrate proteins and modulates their conformations to carry out many cellular processes. However, the fundamental mechanisms underlying the diverse pivotal roles of Cdc48 remain unknown. Almost all AAA proteins form a ring-shaped structure with a conserved aromatic amino acid residue that is essential for proper function. The threading mechanism hypothesis suggests that this residue guides the intrusion of substrate proteins into a narrow pore of the AAA ring, thereby becoming unfolded. By contrast, the aromatic residue in one of the two AAA rings of Cdc48 has been eliminated through evolution. Here, we show that artificial retrieval of this aromatic residue in Cdc48 is lethal, and essential features to support the threading mechanism are required to exhibit the lethal phenotype. In particular, genetic and biochemical analyses of the Cdc48 lethal mutant strongly suggested that when in complex with the 20S proteasome, essential proteins are abnormally forced to thread through the Cdc48 pore to become degraded, which was not detected in wild-type Cdc48. Thus, the widely applicable threading model is less effective for wild-type Cdc48; rather, Cdc48 might function predominantly through an as-yet-undetermined mechanism.

  5. Student-Athlete Perceptions of a Summer Pre-Enrollment Experience at an NCAA Division I-AAA Institution

    Science.gov (United States)

    Dalgety, Michael Franklin

    2012-01-01

    The purpose of this exploratory qualitative study was to examine student-athlete perceptions of the role of summer pre-enrollment in their adjustment and transition to college. The study focused on student-athletes who received athletically-related financial aid at a National Collegiate Athletic Association (NCAA) Division I-AAA institution. The…

  6. Anonymous voting for multi-dimensional CV quantum system

    International Nuclear Information System (INIS)

    Shi Rong-Hua; Xiao Yi; Shi Jin-Jing; Guo Ying; Lee, Moon-Ho

    2016-01-01

    We investigate the design of anonymous voting protocols, CV-based binary-valued ballot and CV-based multi-valued ballot with continuous variables (CV) in a multi-dimensional quantum cryptosystem to ensure the security of voting procedure and data privacy. The quantum entangled states are employed in the continuous variable quantum system to carry the voting information and assist information transmission, which takes the advantage of the GHZ-like states in terms of improving the utilization of quantum states by decreasing the number of required quantum states. It provides a potential approach to achieve the efficient quantum anonymous voting with high transmission security, especially in large-scale votes. (paper)

  7. SMYD2 promoter DNA methylation is associated with abdominal aortic aneurysm (AAA) and SMYD2 expression in vascular smooth muscle cells.

    Science.gov (United States)

    Toghill, Bradley J; Saratzis, Athanasios; Freeman, Peter J; Sylvius, Nicolas; Bown, Matthew J

    2018-01-01

    Abdominal aortic aneurysm (AAA) is a deadly cardiovascular disease characterised by the gradual, irreversible dilation of the abdominal aorta. AAA is a complex genetic disease but little is known about the role of epigenetics. Our objective was to determine if global DNA methylation and CpG-specific methylation at known AAA risk loci is associated with AAA, and the functional effects of methylation changes. We assessed global methylation in peripheral blood mononuclear cell DNA from 92 individuals with AAA and 93 controls using enzyme-linked immunosorbent assays, identifying hyper-methylation in those with large AAA and a positive linear association with AAA diameter ( P  AAA risk loci identified in genome-wide association studies, using bisulphite next-generation sequencing (NGS) in vascular smooth muscle cells (VSMCs) taken from aortic tissues of 44 individuals (24 AAAs and 20 controls). In IL6R , 2 CpGs were hyper-methylated ( P  = 0.0145); in ERG , 13 CpGs were hyper-methylated ( P  = 0.0005); in SERPINB9 , 6 CpGs were hypo-methylated ( P  = 0.0037) and 1 CpG was hyper-methylated ( P  = 0.0098); and in SMYD2 , 4 CpGs were hypo-methylated ( P  = 0.0012).RT-qPCR was performed for each differentially methylated gene on mRNA from the same VSMCs and compared with methylation. This analysis revealed downregulation of SMYD2 and SERPINB9 in AAA, and a direct linear relationship between SMYD2 promoter methylation and SMYD2 expression ( P  = 0.038). Furthermore, downregulation of SMYD2 at the site of aneurysm in the aortic wall was further corroborated in 6 of the same samples used for methylation and gene expression analysis with immunohistochemistry. This study is the first to assess DNA methylation in VSMCs from individuals with AAA using NGS, and provides further evidence there is an epigenetic basis to AAA. Our study shows that methylation status of the SMYD2 promoter may be linked with decreased SMYD2 expression in disease pathobiology. In

  8. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo.

    Science.gov (United States)

    Ottosson, R O; Karlsson, A; Behrens, C F

    2010-08-21

    The pencil beam dose calculation method is frequently used in modern radiation therapy treatment planning regardless of the fact that it is documented inaccurately for cases involving large density variations. The inaccuracies are larger for higher beam energies. As a result, low energy beams are conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6 MV. AAA and MC both predict similar perturbations in dose distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement with MC, but even a small overestimation of the dose level by the algorithm might lead to a large part of the PTV being underdosed. It is advisable to use low energy as a

  9. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    Energy Technology Data Exchange (ETDEWEB)

    Ottosson, R O; Karlsson, A; Behrens, C F, E-mail: riolot01@heh.regionh.d [Department of Oncology (R), Division of Radiophysics (52AA), Copenhagen University Hospital Herlev, Herlev Ringvej 75, DK-2730 Herlev (Denmark)

    2010-08-21

    The pencil beam dose calculation method is frequently used in modern radiation therapy treatment planning regardless of the fact that it is documented inaccurately for cases involving large density variations. The inaccuracies are larger for higher beam energies. As a result, low energy beams are conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6 MV. AAA and MC both predict similar perturbations in dose distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement with MC, but even a small overestimation of the dose level by the algorithm might lead to a large part of the PTV being underdosed. It is advisable to use low energy as a

  10. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    International Nuclear Information System (INIS)

    Ottosson, R O; Karlsson, A; Behrens, C F

    2010-01-01

    The pencil beam dose calculation method is frequently used in modern radiation therapy treatment planning regardless of the fact that it is documented inaccurately for cases involving large density variations. The inaccuracies are larger for higher beam energies. As a result, low energy beams are conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6 MV. AAA and MC both predict similar perturbations in dose distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement with MC, but even a small overestimation of the dose level by the algorithm might lead to a large part of the PTV being underdosed. It is advisable to use low energy as a

  11. Thin-layer drying of tomato (Lycopersicum esculentum Mill. cv. Rio Grande) slices in a convective hot air dryer

    Energy Technology Data Exchange (ETDEWEB)

    Demiray, Engin; Tulek, Yahya [Pamukkale University, Engineering Faculty, Department of Food Engineering, Kinikli, Denizli (Turkey)

    2012-05-15

    The effects of different drying temperatures on the drying kinetics of tomato slices were investigated using a cabinet-type dryer. The experimental drying data were fitted best to the to the Page and Modified Page models apart from other theoretical models to predict the drying kinetics. The effective moisture diffusivities varied from 1.015 x 10{sup -9} to 2.650 x 10{sup -9} m {sup 2} s {sup -1}over the temperature range studied, and activation energy was 22.981 kJ mol {sup -1}. (orig.)

  12. [Incidence and risk factors of ischemic colitis after AAA repair in our cohort of patients from 2005 through 2009].

    Science.gov (United States)

    Biros, E; Staffa, R

    2011-12-01

    Using retrospective analysis, we sought to investigate the incidence, risk factors and therapeutic outcomes of ischemic colitis in patients after surgical and endovascular repair of abdominal aortic aneurysms (AAA). The complete inpatient and outpatient medical records of all patients undergoing surgical or endovascular AAA repair in our Department from January 2005 to December 2009 were retrospectively reviewed. We selected all patients who had developed an acute or chronic form of postoperative large or small bowel ischemia. We carried out data analysis and focused on determining the incidence and risk factors of this complication and the outcomes of its treatment. Two hundred and seven AAA repairs were performed in the 2nd Department of Surgery of St. Anne's University Hospital in Brno and the Faculty of Medicine of Masaryk University in Brno during the studied period. This number includes endovascular AAA repairs (13 patients; 6.3%) as well as one robot-assisted operation, and also the whole clinical spectrum of AAA manifestations, from non-symptomatic forms to ruptured aneurysm forms. The rest of the patients underwent open operation. Bowel ischemia developed in a total of 11 patients (5.3 %), who all underwent open AAA repair. Six of these patients presented with non-ruptured AAA and the remaining 5 with ruptured AAA. In 3 patients, bowel ischemia was diagnosed with a delay of several months from the original revascularization operation in the clinical form of postischemic stricture of the large bowel (2 patients) or postischemic colitis (1 patient). 8 patients were diagnosed with acute ischemic colitis affecting an isolated segment of the small bowel in one patient, extended segments of the large bowel (descending colon + sigmoid colon + rectum) in 2 patients, and typically the descending and sigmoid colon in 5 patients. None of the three patients with late manifestation of ischemic colitis died. Of the 8 patients with acute presentation, resection of the

  13. Instant OpenCV for iOS

    CERN Document Server

    Kornyakov, Kirill

    2013-01-01

    Filled with practical, step-by-step instructions and clear explanations for the most important and useful tasks. This book uses a very practical approach, with each recipe and their associated sample projects or examples focusing on a particular aspect of the technology.This book is intended for OpenCV developers who are interested in porting their applications to the iOS platform. Basic experience with OpenCV, computer vision, Objective C, and other iOS tools is encouraged.

  14. Inter-ring rotations of AAA ATPase p97 revealed by electron cryomicroscopy.

    Science.gov (United States)

    Yeung, Heidi O; Förster, Andreas; Bebeacua, Cecilia; Niwa, Hajime; Ewens, Caroline; McKeown, Ciarán; Zhang, Xiaodong; Freemont, Paul S

    2014-03-05

    The type II AAA+ protein p97 is involved in numerous cellular activities, including endoplasmic reticulum-associated degradation, transcription activation, membrane fusion and cell-cycle control. These activities are at least in part regulated by the ubiquitin system, in which p97 is thought to target ubiquitylated protein substrates within macromolecular complexes and assist in their extraction or disassembly. Although ATPase activity is essential for p97 function, little is known about how ATP binding or hydrolysis is coupled with p97 conformational changes and substrate remodelling. Here, we have used single-particle electron cryomicroscopy (cryo-EM) to study the effect of nucleotides on p97 conformation. We have identified conformational heterogeneity within the cryo-EM datasets from which we have resolved two major p97 conformations. A comparison of conformations reveals inter-ring rotations upon nucleotide binding and hydrolysis that may be linked to the remodelling of target protein complexes.

  15. Conformational changes in the AAA ATPase p97–p47 adaptor complex

    Science.gov (United States)

    Beuron, Fabienne; Dreveny, Ingrid; Yuan, Xuemei; Pye, Valerie E; Mckeown, Ciaran; Briggs, Louise C; Cliff, Matthew J; Kaneko, Yayoi; Wallis, Russell; Isaacson, Rivka L; Ladbury, John E; Matthews, Steve J; Kondo, Hisao; Zhang, Xiaodong; Freemont, Paul S

    2006-01-01

    The AAA+ATPase p97/VCP, helped by adaptor proteins, exerts its essential role in cellular events such as endoplasmic reticulum-associated protein degradation or the reassembly of Golgi, ER and the nuclear envelope after mitosis. Here, we report the three-dimensional cryo-electron microscopy structures at ∼20 Å resolution in two nucleotide states of the endogenous hexameric p97 in complex with a recombinant p47 trimer, one of the major p97 adaptor proteins involved in membrane fusion. Depending on the nucleotide state, we observe the p47 trimer to be in two distinct arrangements on top of the p97 hexamer. By combining the EM data with NMR and other biophysical measurements, we propose a model of ATP-dependent p97(N) domain motions that lead to a rearrangement of p47 domains, which could result in the disassembly of target protein complexes. PMID:16601695

  16. Beneficial effect of statins on total mortality in abdominal aortic aneurysm (AAA) repair.

    Science.gov (United States)

    Mathisen, Sven Ross; Abdelnoor, Michael

    2017-10-01

    In this single center, retrospective cohort study we wished to compare early and total mortality for all patients treated for abdominal aortic aneurysms (AAA) with open surgery who were taking statins compared to those who were not. A cohort of 640 patients with AAA was treated with open surgery between 1999 and 2012. Patients were consecutively recruited from a source population of 390,000; 21.3% were female, and the median age was 73 years. The median follow-up was 3.93 years, with an interquartile range of 1.79-6.58 years. The total follow-up was 2855 patient-years. An explanatory strategy was used. The propensity score (PS) was implemented to control for selection bias and confounders. The crude effect of statin use showed a 78% reduction of the 30-day mortality. A stratified analysis using the Mantel-Haenszel method on quintiles of the PS gave an adjusted effect of the odds ratio equal to 0.43 (95% CI: 0.18-0.96), indicating a 57% reduction of the 30-day mortality for statin users. The adjusted rate ratio was 0.62 (95% CI: 0.45-0.83), indicating a reduction of long-term mortality of 38% for statin users compared to non-users for a median follow-up of 3.93 years. This retrospective cohort study showed a significant beneficial effect of statin use on early and long-term survival for patients treated with open surgery. To be conclusive, our results need to be replicated by a randomized clinical trial.

  17. The AAA-ATPase NVL2 is a telomerase component essential for holoenzyme assembly

    Energy Technology Data Exchange (ETDEWEB)

    Her, Joonyoung [Departments of Biology and Integrated Omics for Biomedical Science, Yonsei University, Seoul 120-749 (Korea, Republic of); Chung, In Kwon, E-mail: topoviro@yonsei.ac.kr [Departments of Biology and Integrated Omics for Biomedical Science, Yonsei University, Seoul 120-749 (Korea, Republic of)

    2012-01-20

    Highlights: Black-Right-Pointing-Pointer Identification of the AAA-ATPase NVL2 as a novel hTERT-interacting protein. Black-Right-Pointing-Pointer NVL2 associates with catalytically active telomerase via an interaction with hTERT. Black-Right-Pointing-Pointer NVL2 is a telomerase component essential for holoenzyme assembly. Black-Right-Pointing-Pointer ATP-binding activity of NVL2 is required for hTERT binding and telomerase assembly. -- Abstract: Continued cell proliferation requires telomerase to maintain functional telomeres that are essential for chromosome integrity. Although the core enzyme includes a telomerase reverse transcriptase (TERT) and a telomerase RNA component (TERC), a number of auxiliary proteins have been identified to regulate telomerase assembly, localization, and enzymatic activity. Here we describe the characterization of the AAA-ATPase NVL2 as a novel hTERT-interacting protein. NVL2 interacts and co-localizes with hTERT in the nucleolus. NLV2 is also found in association with catalytically competent telomerase in cell lysates through an interaction with hTERT. Depletion of endogenous NVL2 by small interfering RNA led to a decrease in hTERT without affecting the steady-state levels of hTERT mRNA, thereby reducing telomerase activity, suggesting that NVL2 is an essential component of the telomerase holoenzyme. We also found that ATP-binding activity of NVL2 is required for hTERT binding as well as telomerase assembly. Our findings suggest that NVL2, in addition to its role in ribosome biosynthesis, is essential for telomerase biogenesis and provides an alternative approach for inhibiting telomerase activity in cancer.

  18. Grand unification: quo vadis domine

    International Nuclear Information System (INIS)

    Senjanovic, G.

    1985-01-01

    The present theoretical and experimental situation with grand unification is summarized. The issues of proton decay and the Weinberg angle are addressed, going through the predictions of both the standard SU(5) theory and its supersymmetric extension. The SO(10) theory, which provides a minimal one family model, is then studied. The gravitational characteristics of domain walls and strings are then discussed. It is argued that there is a need to go beyond SO(10) in order to incorporate a unified picture of families. This leads to the prediction of mirror fermions, whose physics is analyzed. 31 refs

  19. Graafikatriennaali grand prix Korea kunstnikule

    Index Scriptorium Estoniae

    1998-01-01

    Tallinna XI graafikatriennaali rahvusvaheline žürii andis grand prix korea kunstnikule Chung¡Sang-Gonile, kolm võrdset preemiat - soome kunstnikele Anita Jensenile ja Tapani Mikkonenile ning jaapani kunstnikule Estuko Obatale. Eesti Kunstimuuseumi preemia - Wendy Swallow. Tallinna linna preemia ja Ivar Luki sponsoripreemia - Walter Jule. Sponsoripreemiad : Paletti Eesti AS preemia - Inga Heamägi; Rannila Profiili preemia - Mojca Zlokarnik; UNDP preemia - Andrea Juan. Rotermanni soolalao arhitektuuri- ja kunstikeskuse diplom - Lis Ingram, Heli Päivikki Kurunsaari, Randi Strand, Wendy Swallow

  20. Grand Challenges of Enterprise Integration

    Energy Technology Data Exchange (ETDEWEB)

    Brosey, W.D; Neal, R.E.; Marks, D.

    2001-04-01

    Enterprise Integration connects and combines people, processes, systems, and technologies to ensure that the right people and the right processes have the right information and the right resources at the right time. A consensus roadmap for Technologies for Enterprise Integration was created as part of an industry/government/academia partnership in the Integrated Manufacturing Technology Initiative (IMTI). Two of the grand challenges identified by the roadmapping effort will be addressed here--Customer Responsive Enterprises and Totally Connected Enterprises. Each of these challenges is briefly discussed as to the current state of industry and the future vision as developed in the roadmap.

  1. Grand Challenges for Environmental Magnetism

    Science.gov (United States)

    Verosub, K. L.

    2009-05-01

    The development of new, inexpensive, and rapid geochemical methods for determining the ages of geologic materials, their elemental composition, and their isotopic ratios over a broad array of elements puts into sharp focus the question: What information can environmental magnetic methods provide that can't be obtained using these other methods? Because iron is ubiquitous in the Earth's crust and because it exists in so many different forms, a discipline that looks in detail at iron-bearing minerals does have the potential to make significant contributions to the study of surficial processes. However, to reach that potential requires the development of new environmental magnetic methods. I would like to put forward three Grand Challenges for environmental magnetism that have the potential to move the field forward to a new level of scientific sophistication and that will allow environmental magnetists to compete successfully in a world increasingly dominated by geochemists. The first Grand Challenge is the development of new techniques that lead to the direct and unambiguous identification of the full suite of magnetic minerals. For many environmental magnetic applications, the key magnetic minerals are not just magnetite and hematite but also iron oxy-hydroxides (goethite, lepidocrocite, akaganeite, ferrihydrite), carbonates (siderite) and sulfides (pyrrhotite and greigite) as well as compounds involving iron and other transition metals (cobalt and nickel). The second Grand Challenge is the development of new analytical methods that provide specific quantitative values for the amount of each magnetic mineral present in a sample. One promising approach to this problem is the application of two- or three-component multivariate analysis to arrays of downcore environmental magnetic parameters. The third Grand Challenge is the development of new ways of determining, not just the average values, but the actual distributions of grain sizes and coercivities of each mineral

  2. The Grand Challenges of Nanotechnology

    International Nuclear Information System (INIS)

    Lane, Neal

    2001-01-01

    Amazing breakthroughs and advances continue to be made in nanoscale science and engineering and the rapidly emerging field of nanotechnology, including near-commercial applications in biomedicine, computing and environmental protection. The National Nanotechnology Initiative, begun by the Clinton Administration has placed nanoscale research on a new funding trajectory. But, many 'grand challenges' must be overcome, technical ones as well as those related to funding, science and technology workforce, and the need for stronger collaboration across discipline, organizations, government agencies and with other countries

  3. The role of topolins in micropropagation and somaclonal variation of banana cultivars ´Williams´ and ´Grand Naine´ (Musa spp. AAA)

    Czech Academy of Sciences Publication Activity Database

    Bairu, M. W.; Stirk, W.A.; Doležal, Karel; van Staden, J.

    2008-01-01

    Roč. 95, č. 3 (2008), s. 373-379 ISSN 0167-6857 R&D Projects: GA ČR GA522/06/0108 Institutional research plan: CEZ:AV0Z50380511 Keywords : Abnormality index * Banana * Micropropagation Subject RIV: ED - Physiology Impact factor: 1.017, year: 2008

  4. Plant regeneration of Brassica oleracea subsp. italica (Broccoli) CV ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-06-03

    Jun 3, 2009 ... Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul. Ehsan, Malaysia. Accepted 20 March, 2009. Hypocotyls and shoot tips were used as explants in in vitro plant regeneration of broccoli (Brassica oleracea subsp.italica) cv. Green Marvel.

  5. SU-F-T-622: Comparative Analysis of Pencil Beam and Anisotropic Analytical Algorithm (AAA) for Stereotactic Body Radiation Therapy (SBRT) of Thoracic Spine

    Energy Technology Data Exchange (ETDEWEB)

    Badkul, R; Nicolai, W; Pokhrel, D; Jiang, H; Wang, F; Lominskac, C [University of Kansas Medical Center, Kansas City, KS (United States); Ramanjappa, T [S. K. University, Anantapur, AP (India)

    2016-06-15

    Purpose: To compare the impact of Pencil Beam(PB) and Anisotropic Analytic Algorithm(AAA) dose calculation algorithms on OARs and planning target volume (PTV) in thoracic spine stereotactic body radiation therapy (SBRT). Methods: Ten Spine SBRT patients were planned on Brainlab iPlan system using hybrid plan consisting of 1–2 non-coplanar conformal-dynamic arcs and few IMRT beams treated on NovalisTx with 6MV photon. Dose prescription varied from 20Gy to 30Gy in 5 fractions depending on the situation of the patient. PB plans were retrospectively recalculated using the Varian Eclipse with AAA algorithm using same MUs, MLC pattern and grid size(3mm).Differences in dose volume parameters for PTV, spinal cord, lung, and esophagus were analyzed and compared for PB and AAA algorithms. OAR constrains were followed per RTOG-0631. Results: Since patients were treated using PB calculation, we compared all the AAA DVH values with respect to PB plan values as standard, although AAA predicts the dose more accurately than PB. PTV(min), PTV(Max), PTV(mean), PTV(D99%), PTV(D90%) were overestimated with AAA calculation on average by 3.5%, 1.84%, 0.95%, 3.98% and 1.55% respectively as compared to PB. All lung DVH parameters were underestimated with AAA algorithm mean deviation of lung V20, V10, V5, and 1000cc were 42.81%,19.83%, 18.79%, and 18.35% respectively. AAA overestimated Cord(0.35cc) by mean of 17.3%; cord (0.03cc) by 12.19% and cord(max) by 10.5% as compared to PB. Esophagus max dose were overestimated by 4.4% and 5cc by 3.26% for AAA algorithm as compared to PB. Conclusion: AAA overestimated the PTV dose values by up to 4%.The lung DVH had the greatest underestimation of dose by AAA versus PB. Spinal cord dose was overestimated by AAA versus PB. Given the critical importance of accuracy of OAR and PTV dose calculation for SBRT spine, more accurate algorithms and validation of calculated doses in phantom models are indicated.

  6. Avaliação da cv. Merlot para elaboração de vinho tinto Evaluation of cv. Merlot to make red wine

    Directory of Open Access Journals (Sweden)

    Luiz Antenor Rizzon

    2003-12-01

    Full Text Available A uva Merlot é uma das responsáveis pelas características dos vinhos tintos de Saint Émillion, região de Bordeaux, França. Foi introduzida no Rio Grande do Sul através da Estação Agronômica de Porto Alegre. É, atualmente, uma das cultivares tinta de Vitis vinifera L. com maior volume de produção da Serra Gaúcha. É utilizada para a elaboração de vinho tinto para ser consumido jovem. O objetivo do presente trabalho foi avaliar o potencial enológico da cv. Merlot para a elaboração de vinho tinto fino. Para isso, realizaram-se estudos para caracterizar o cacho, o mosto e o vinho nas safras de 1987 a 1994, em Bento Gonçalves, RS. Os resultados evidenciaram que a cv. Merlot tem cacho com peso médio de 161,9g e baga esférica e pequena, pesando 1,61g. No mosto, o valor médio de açúcar é de 18,4ºBrix; o da acidez total, de 104meqL-1; e o do pH, de 3,21. O vinho se caracteriza por ter teores médios de 10,8% v/v de álcool; 70,3meqL-1 de acidez titulável; e pH de 3,49. Sob o aspecto sensorial, o vinho Merlot apresenta cor vermelho-violáceo com boa intensidade. Gustativamente distingue-se pela maciez, equilíbrio e fineza.Merlot is a traditional grape of the Saint Émillion wines, Bordeaux region - France. It was introduced into, the State of Rio Grande do Sul by the Estação Agronômica de Porto Alegre, Brazil. Today, of the red grape cultivars it shows the largest production in the Serra Gaúcha region. The purpose of this work was to evaluate the enological potential of Merlot grapes for red winemaking. To achieve this purpose, variables related to berry clusters, must and wine were measured between 1987 and 1994 vintages, from Merlot vineyards located in the municipality of Bento Gonçalves. The results showed that Merlot grapes have clusters of medium size (161.9g and small berries (1.61g. The must showed adequate sugar level (18.4ºBrix, high titratable acidity (104mqL-1 and medium pH 3.21. The wine Merlot had an

  7. Avaliação da cv. Tannat para elaboração de vinho tinto Evaluation of cv. Tannat to elaborate red wine

    Directory of Open Access Journals (Sweden)

    Luiz Antenor Rizzon

    2004-06-01

    Full Text Available A uva Tannat, originária do Sudoeste da França, foi introduzida no Rio Grande do Sul, pela Estação Experimental de Caxias do Sul. É uma cultivar difundida na Serra Gaúcha para elaboração de vinho tinto, utilizado para corte e para a produção de vinho varietal. O objetivo do presente trabalho foi avaliar o potencial enológico da cv. Tannat para a elaboração de vinho tinto fino nessa região vitícola. Para isso, realizaram-se estudos para caracterizar o cacho, o mosto e o vinho nas safras de 1987 a 1994, a partir de um vinhedo localizado em Bento Gonçalves, RS. Os resultados evidenciaram que a cv. Tannat tem cacho com peso médio de 216,1g e baga esférica pequena (1,54g. O mosto apresenta teor elevado de acidez titulável (155meq/L-1. O vinho se caracteriza por teores elevados em: polifenóis totais (I 280= 43,0, taninos (1,9g/L, antocianinas (539mg/L, potássio (1.856mg/L-1 e soma dos álcoois superiores (512mg/L-1. As características sensoriais do vinho Tannat na Serra Gaúcha evidenciam uma cor vermelha-violácea intensa, tânicos, nervosos, relativamente duros e aromas de frutas vermelhas. É recomendável para corte com outros vinhos tintos finos para intensificar sua cor e estrutura e também para a produção de vinho varietal podendo melhorar com o amadurecimento em barricas de carvalho.The Tannat grape is native to the Southwest of France, and was introduced into the State of Rio Grande do Sul by the Caxias do Sul Experimental Station. It is a cultivar diffused in the Serra Gaucha region with the purpose of elaborating varietal red wine for blending. The purpose of this work was to evaluate the enological potential of the Tannat grape for red wine in this region. To achieve this objective, variables related to the berries, clusters, must, and wine were evaluated from the 1987 to 1994 vintages, in a vineyard established in Bento Gonçalves, RS, Brazil. The results showed that Tannat grapes have large clusters (216.1g and

  8. Clinical implications in the use of the PBC algorithm versus the AAA by comparison of different NTCP models/parameters.

    Science.gov (United States)

    Bufacchi, Antonella; Nardiello, Barbara; Capparella, Roberto; Begnozzi, Luisa

    2013-07-04

    Retrospective analysis of 3D clinical treatment plans to investigate qualitative, possible, clinical consequences of the use of PBC versus AAA. The 3D dose distributions of 80 treatment plans at four different tumour sites, produced using PBC algorithm, were recalculated using AAA and the same number of monitor units provided by PBC and clinically delivered to each patient; the consequences of the difference on the dose-effect relations for normal tissue injury were studied by comparing different NTCP model/parameters extracted from a review of published studies. In this study the AAA dose calculation is considered as benchmark data. The paired Student t-test was used for statistical comparison of all results obtained from the use of the two algorithms. In the prostate plans, the AAA predicted lower NTCP value (NTCPAAA) for the risk of late rectal bleeding for each of the seven combinations of NTCP parameters, the maximum mean decrease was 2.2%. In the head-and-neck treatments, each combination of parameters used for the risk of xerostemia from irradiation of the parotid glands involved lower NTCPAAA, that varied from 12.8% (sd=3.0%) to 57.5% (sd=4.0%), while when the PBC algorithm was used the NTCPPBC's ranging was from 15.2% (sd=2.7%) to 63.8% (sd=3.8%), according the combination of parameters used; the differences were statistically significant. Also NTCPAAA regarding the risk of radiation pneumonitis in the lung treatments was found to be lower than NTCPPBC for each of the eight sets of NTCP parameters; the maximum mean decrease was 4.5%. A mean increase of 4.3% was found when the NTCPAAA was calculated by the parameters evaluated from dose distribution calculated by a convolution-superposition (CS) algorithm. A markedly different pattern was observed for the risk relating to the development of pneumonitis following breast treatments: the AAA predicted higher NTCP value. The mean NTCPAAA varied from 0.2% (sd = 0.1%) to 2.1% (sd = 0.3%), while the mean NTCPPBC

  9. SU-E-T-122: Anisotropic Analytical Algorithm (AAA) Vs. Acuros XB (AXB) in Stereotactic Treatment Planning

    International Nuclear Information System (INIS)

    Mynampati, D; Scripes, P Godoy; Kuo, H; Yaparpalvi, R; Tome, W

    2015-01-01

    Purpose: To evaluate dosimetric differences between superposition beam model (AAA) and determinant photon transport solver (AXB) in lung SBRT and Cranial SRS dose computations. Methods: Ten Cranial SRS and ten Lung SBRT plans using Varian, AAA -11.0 were re-planned using Acuros -XB-11.0 with fixed MU. 6MV photon Beam model with HD120-MLC used for dose calculations. Four non-coplanar conformal arcs used to deliver 21Gy or 18Gy to SRS targets (0.4 to 6.2cc). 54Gy (3Fractions) or 50Gy (5Fractions) was planned for SBRT targets (7.3 to 13.9cc) using two VAMT non-coplanar arcs. Plan comparison parameters were dose to 1% PTV volume (D1), dose to 99% PTV volume( D99), Target mean (Dmean), Conformity index (ratio of prescription isodose volume to PTV), Homogeneity Index [ (D2%-D98%)/Dmean] and R50 (ratio of 50% of prescription isodose volume to PTV). OAR parameters were Brain volume receiving 12Gy dose (V12Gy) and maximum dose (D0.03) to Brainstem for SRS. For lung SBRT, maximum dose to Heart and Cord, Mean lung dose (MLD) and volume of lung receiving 20Gy (V20Gy) were computed. PTV parameters compared by percentage difference between AXB and AAA parameters. OAR parameters and HI compared by absolute difference between two calculations. For analysis, paired t-test performed over the parameters. Results: Compared to AAA, AXB SRS plans have on average 3.2% lower D99, 6.5% lower CI and 3cc less Brain-V12. However, AXB SBRT plans have higher D1, R50 and Dmean by 3.15%, 1.63% and 2.5%. For SRS and SBRT, AXB plans have average HI 2 % and 4.4% higher than AAA plans. In both techniques, all other parameters vary within 1% or 1Gy. In both sets only two parameters have P>0.05. Conclusion: Even though t-test results signify difference between AXB and AAA plans, dose differences in dose estimations by both algorithms are clinically insignificant

  10. Google Earth Grand Tour Themes

    Science.gov (United States)

    De Paor, D. G.; Whitmeyer, S. J.; Bentley, C.; Dordevic, M. M.

    2014-12-01

    As part of an NSF TUES Type 3 project entitled "Google Earth for Onsite and Distance Education (GEODE)," we are assembling a "Grand Tour" of locations on Earth and other terrestrial bodies that every geoscience student should know about and visit at least in virtual reality. Based on feedback from colleagues at previous meetings, we have identified nine Grand Tour themes: "Plates and Plumes," "Rocks and Regions," "Geology Through Time," "The Mapping Challenge*," "U.S. National Parks*," "The Magical Mystery Tour*," "Resources and Hazards," "Planets and Moons," and "Top of the Pops." Themes marked with an asterisk are most developed at this stage and will be demonstrated in real time. The Mapping Challenge invites students to trace geological contacts, measure bedding strike and dip and the plunge, trend, and facing of a fold. There is an advanced tool for modeling periclinal folds. The challenge is presented in a game-like format with an emphasis on puzzle-solving that will appeal to students regardless of gender. For the tour of U.S. national parks, we divided the most geologically important parks into four groups—Western Pacific, West Coast, Rockies, and East Coast. We are combining our own team's GigaPan imagery with imagery already available on the Internet. There is a great deal of imagery just waiting to be annotated for geological education purposes. The Magical Mystery Tour takes students to Google Streetview locations selected by instructors. Students are presented with questions or tasks and are given automatic feedback. Other themes are under development. Within each theme, we are crowd-sourcing contributions from colleagues and inviting colleagues to vote for or against proposed locations and student interactions. The GEODE team includes the authors and: Heather Almquist, Stephen Burgin, Cinzia Cervato, Gene Cooper, Paul Karabinos, Terry Pavlis, Jen Piatek, Bill Richards, Jeff Ryan, Ron Schott, Kristen St. John, and Barb Tewksbury.

  11. Next Generation Innovation Policy and Grand Challenges

    NARCIS (Netherlands)

    Kuhlmann, Stefan; Rip, Arie

    2018-01-01

    The paper explores transformative ways to address Grand Challenges, while locating them in a broader diagnosis of ongoing changes. Coping with Grand Challenges is a challenge in its own right, for policy as well as for science, technology, and innovation actors. The paper presents building blocks

  12. Proton hexality in local grand unification

    Energy Technology Data Exchange (ETDEWEB)

    Foerste, Stefan; Nilles, Hans Peter [Bonn Univ. (Germany). Bethe Center for Theoretical Physics and Physikalisches Institut; Ramos-Sanchez, Saul [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Vaudrevange, Patrick K.S. [Muenchen Univ. (Germany). Arnold Sommerfeld Center for Theoretical Physics

    2010-07-15

    Proton hexality is a discrete symmetry that avoids the problem of too fast proton decay in the supersymmetric extension of the standard model. Unfortunately it is inconsistent with conventional grand unification. We show that proton hexality can be incorporated in the scheme of ''Local Grand Unification'' discussed in the framework of model building in (heterotic) string theory. (orig.)

  13. The Protective Effects of Diabetes Mellitus on Post-EVAR AAA Growth and Reinterventions.

    Science.gov (United States)

    Png, Chien Yi M; Tadros, Rami O; Kang, Ming; Beckerman, William E; Tardiff, Melissa L; Vouyouka, Ageliki G; Marin, Michael L; Faries, Peter L

    2017-08-01

    This study aims to investigate the effect of diabetes on post-endovascular aneurysm repairs (EVARs) of abdominal aortic aneurysms (AAAs). A total of 1,479 consecutive patients who underwent AAA EVAR were reviewed. The cohorts were divided based on their diabetes status and compared. Preoperative demographic and comorbidity data were analyzed using the t-test and chi-squared test, whereas post-EVAR outcomes were analyzed using Probit multivariate model, followed by Kaplan-Meier survival curve and Cox regression. Of our 1,479 patients, 993 met inclusion criteria. One hundred eighty-three were diabetics (18.4%) compared with 810 nondiabetics (81.6%). Coronary artery disease (CAD; diabetics: 70.49%, nondiabetics: 60.76%, P = 0.014) and hypertension (HTN; diabetics: 90.16%, nondiabetics: 79.46%, P = 0.0008) were the only comorbidities analyzed, including follow-up length, which had any significant differences between the diabetic and nondiabetic groups. Probit multivariate analysis using a combined cohort follow-up mean of 51 months showed a significant decrease in aneurysm sac enlargement in diabetic patients (diabetics: 13.11%, nondiabetics: 19.43%, model estimate: 0.3058; 95% confidence interval [CI]: 0.0486-0.5629, Pr > ChiSq = 0.0198) and trended toward significantly fewer reinterventions (diabetics: 23.50%, nondiabetics: 28.41%, model estimate: 0.1990; 95% CI: -0.0262 to 0.4243, Pr > ChiSq = 0.0833). In the Cox regressions, diabetes had a significant protective factor on reinterventions (hazard ratio [HR]: 0.697, Pr > ChiSq = 0.0151), and was trending toward significance for aneurysm sac enlargement (HR: 0.750, Pr > ChiSq = 0.1961). There was no significant difference across diabetic status in any other outcomes, including mortality and endoleak occurrence. Although a higher proportion of diabetic patients present with HTN and CAD, they have decreased long-term rates of aneurysm sac enlargement after EVAR. As a result, this cohort trends

  14. Target dose study of effects of changes in the AAA calculation resolution on lung SABR plan

    International Nuclear Information System (INIS)

    Kim, Dae Il; Son, Sang Jun; Ahn, Bum Seok; Jung, Chi Hoon; Yoo, Suk Hyun

    2014-01-01

    Changing the calculation grid of AAA in Lung SABR plan and to analyze the changes in target dose, and investigated the effects associated with it, and considered a suitable method of application. 4D CT image that was used to plan all been taken with Brilliance Big Bore CT (Philips, Netherlands) and in Lung SABR plan(Eclipse TM ver10.0.42, Varian, the USA), use anisotropic analytic algorithm(AAA, ver.10, Varian Medical Systems, Palo Alto, CA, USA) and, was calculated by the calculation grid 1.0, 3.0, 5.0 mm in each Lung SABR plan. Lung SABR plan of 10 cases are using each of 1.0 mm, 3.0 mm, 5.0 mm calculation grid, and in case of use a 1.0 mm calculation grid V98 of the prescribed dose is about 99.5%±1.5%, Dmin of the prescribed dose is about 92.5±1.5% and Homogeneity Index(HI) is 1.0489±0.0025. In the case of use a 3.0 mm calculation grid V98 dose of the prescribed dose is about 90±4.5% , Dmin of the prescribed dose is about 87.5±3% and HI is about 1.07±1. In the case of use a 5.0 mm calculation grid V98 dose of the prescribed dose is about 63±15%, Dmin of the prescribed dose is about 83±4% and HI is about 1.13±0.2, respectively. The calculation grid of 1.0 mm is better improves the accuracy of dose calculation than using 3.0 mm and 5.mm, although calculation times increase in the case of smaller PTV relatively. As lung, spread relatively large and low density and small PTV, it is considered and good to use a calculation grid of 1.0 mm

  15. Obesity is not an independent risk factor for adverse perioperative and long-term clinical outcomes following open AAA repair or EVAR.

    Science.gov (United States)

    Park, Brian; Dargon, Phong; Binette, Christopher; Babic, Bruna; Thomas, Tina; Divinagracia, Thomas; Dahn, Michael S; Menzoian, James O

    2011-10-01

    Moderate (body mass index [BMI] ≥30) and morbid obesity (BMI ≥35) is increasing at an alarming rate in vascular surgery patients. The objective of this study was to determine the impact of obesity on perioperative and long-term clinical outcomes following open abdominal aortic aneurysm (AAA) repair or endovascular aneurysm repair (EVAR). This review includes patients that underwent open AAA repair (n = 403) or EVAR (n = 223) from 1999 to 2009. Specific patient characteristics such as comorbid diseases, medications, and body mass index (BMI) were assessed. Specific perioperative outcomes such as length of stay, myocardial infarctions, and mortality were reviewed. In addition, long-term outcomes such as rates of reintervention, permanent renal dysfunction, and mortality beyond 30 days were also assessed. The incidence of obesity in open AAA patients was 25.3% (documented incidence 1.5%) and for EVAR was 24.6% (documented incidence 4%). Moderate and morbid obesity was associated with longer intensive care unit (ICU) admissions for both open AAA or EVAR patients (P AAA repair or EVAR (P > .05). Similarly, moderate and morbid obesity was not associated with significant differences in rates of reintervention, permanent renal dysfunction, and mortality beyond 30 days for patients undergoing open AAA repair or EVAR (P > .05). The results of this study indicate that moderate and morbid obesity are not independently associated with adverse perioperative and long-term clinical outcomes for patients undergoing open AAA repair or EVAR. Therefore, either open AAA repair or EVAR can be accomplished safely in moderately obese and morbidly obese patients.

  16. Analysis of the cooperative ATPase cycle of the AAA+ chaperone ClpB from Thermus thermophilus by using ordered heterohexamers with an alternating subunit arrangement.

    Science.gov (United States)

    Yamasaki, Takashi; Oohata, Yukiko; Nakamura, Toshiki; Watanabe, Yo-hei

    2015-04-10

    The ClpB/Hsp104 chaperone solubilizes and reactivates protein aggregates in cooperation with DnaK/Hsp70 and its cofactors. The ClpB/Hsp104 protomer has two AAA+ modules, AAA-1 and AAA-2, and forms a homohexamer. In the hexamer, these modules form a two-tiered ring in which each tier consists of homotypic AAA+ modules. By ATP binding and its hydrolysis at these AAA+ modules, ClpB/Hsp104 exerts the mechanical power required for protein disaggregation. Although ATPase cycle of this chaperone has been studied by several groups, an integrated understanding of this cycle has not been obtained because of the complexity of the mechanism and differences between species. To improve our understanding of the ATPase cycle, we prepared many ordered heterohexamers of ClpB from Thermus thermophilus, in which two subunits having different mutations were cross-linked to each other and arranged alternately and measured their nucleotide binding, ATP hydrolysis, and disaggregation abilities. The results indicated that the ATPase cycle of ClpB proceeded as follows: (i) the 12 AAA+ modules randomly bound ATP, (ii) the binding of four or more ATP to one AAA+ ring was sensed by a conserved Arg residue and converted another AAA+ ring into the ATPase-active form, and (iii) ATP hydrolysis occurred cooperatively in each ring. We also found that cooperative ATP hydrolysis in at least one ring was needed for the disaggregation activity of ClpB. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  17. Expression and genomic analysis of midasin, a novel and highly conserved AAA protein distantly related to dynein

    Directory of Open Access Journals (Sweden)

    Gibbons I R

    2002-07-01

    Full Text Available Abstract Background The largest open reading frame in the Saccharomyces genome encodes midasin (MDN1p, YLR106p, an AAA ATPase of 560 kDa that is essential for cell viability. Orthologs of midasin have been identified in the genome projects for Drosophila, Arabidopsis, and Schizosaccharomyces pombe. Results Midasin is present as a single-copy gene encoding a well-conserved protein of ~600 kDa in all eukaryotes for which data are available. In humans, the gene maps to 6q15 and encodes a predicted protein of 5596 residues (632 kDa. Sequence alignments of midasin from humans, yeast, Giardia and Encephalitozoon indicate that its domain structure comprises an N-terminal domain (35 kDa, followed by an AAA domain containing six tandem AAA protomers (~30 kDa each, a linker domain (260 kDa, an acidic domain (~70 kDa containing 35–40% aspartate and glutamate, and a carboxy-terminal M-domain (30 kDa that possesses MIDAS sequence motifs and is homologous to the I-domain of integrins. Expression of hemagglutamin-tagged midasin in yeast demonstrates a polypeptide of the anticipated size that is localized principally in the nucleus. Conclusions The highly conserved structure of midasin in eukaryotes, taken in conjunction with its nuclear localization in yeast, suggests that midasin may function as a nuclear chaperone and be involved in the assembly/disassembly of macromolecular complexes in the nucleus. The AAA domain of midasin is evolutionarily related to that of dynein, but it appears to lack a microtubule-binding site.

  18. Effect of microgravity simulation using 3D clinostat on cavendish banana (Musa acuminata AAA Group) ripening process

    Science.gov (United States)

    Dwivany, Fenny Martha; Esyanti, Rizkita R.; Prapaisie, Adeline; Puspa Kirana, Listya; Latief, Chunaeni; Ginaldi, Ari

    2016-11-01

    The objective of the research was to determine the effect of microgravity simulation by 3D clinostat on Cavendish banana (Musa acuminata AAA group) ripening process. In this study, physical, physiological changes as well as genes expression were analysed. The result showed that in microgravity simulation condition ripening process in banana was delayed and the MaACOl, MaACSl and MaACS5 gene expression were affected.

  19. Pre-Test Analysis of the MEGAPIE Spallation Source Target Cooling Loop Using the TRAC/AAA Code

    International Nuclear Information System (INIS)

    Bubelis, Evaldas; Coddington, Paul; Leung, Waihung

    2006-01-01

    A pilot project is being undertaken at the Paul Scherrer Institute in Switzerland to test the feasibility of installing a Lead-Bismuth Eutectic (LBE) spallation target in the SINQ facility. Efforts are coordinated under the MEGAPIE project, the main objectives of which are to design, build, operate and decommission a 1 MW spallation neutron source. The technology and experience of building and operating a high power spallation target are of general interest in the design of an Accelerator Driven System (ADS) and in this context MEGAPIE is one of the key experiments. The target cooling is one of the important aspects of the target system design that needs to be studied in detail. Calculations were performed previously using the RELAP5/Mod 3.2.2 and ATHLET codes, but in order to verify the previous code results and to provide another capability to model LBE systems, a similar study of the MEGAPIE target cooling system has been conducted with the TRAC/AAA code. In this paper a comparison is presented for the steady-state results obtained using the above codes. Analysis of transients, such as unregulated cooling of the target, loss of heat sink, the main electro-magnetic pump trip of the LBE loop and unprotected proton beam trip, were studied with TRAC/AAA and compared to those obtained earlier using RELAP5/Mod 3.2.2. This work extends the existing validation data-base of TRAC/AAA to heavy liquid metal systems and comprises the first part of the TRAC/AAA code validation study for LBE systems based on data from the MEGAPIE test facility and corresponding inter-code comparisons. (authors)

  20. Detecting endoleaks after endovascular AAA repair with a minimally invasive, implantable, telemetric pressure sensor: an in vitro study

    International Nuclear Information System (INIS)

    Springer, Fabian; Pfeffer, Joachim-Georg; Schmitz-Rode, Thomas; Schlierf, Roland; Schnakenberg, Uwe; Mahnken, Andreas H.

    2007-01-01

    A feasibility study on a completely digital telemetric pressure sensor (TPS) to detect endoleaks was performed in an in vitro model of an abdominal aortic aneurysm (AAA). An endovascular-stented AAA silicone model with different types (I-III) and sizes (3-11 French) of endoleaks was created and pulsatile pressure was applied with physiological flow and pressure rates [mean intraaortic pressure (IAP): 95-130 mmHg] and different degrees of thrombosis of the aneurysm sac. Aneurysm sac pressure (ASP) was measured with the TPS and with wired pressure sensors (WPS) as a reference. Statistical analysis included paired t-test, Pearson's correlation analysis and Bland-Altman plots. After opening an endoleak, the mean ASP increased significantly (P < 0.0001) from 15 to almost 95% of the mean IAP depending on endoleak type and size. ASP could be measured accurately with the TPS and the WPS. The telemetric and wired ASP increase showed a high Pearson's correlation coefficient (r) for a non-thrombosed (r 0.97) and a thrombosed (r = 0.96) aneurysm sac. In an in vitro silicone model, the newly designed telemetric pressure sensor was able to detect the occurrence of an endoleak in a non-invasive way and might be a valuable device for follow-up of endovascular AAA repair. (orig.)

  1. AAA and AXB algorithms for the treatment of nasopharyngeal carcinoma using IMRT and RapidArc techniques.

    Science.gov (United States)

    Kamaleldin, Maha; Elsherbini, Nader A; Elshemey, Wael M

    2017-09-27

    The aim of this study is to evaluate the impact of anisotropic analytical algorithm (AAA) and 2 reporting systems (AXB-D m and AXB-D w ) of Acuros XB algorithm (AXB) on clinical plans of nasopharyngeal patients using intensity-modulated radiotherapy (IMRT) and RapidArc (RA) techniques. Six plans of different algorithm-technique combinations are performed for 10 patients to calculate dose-volume histogram (DVH) physical parameters for planning target volumes (PTVs) and organs at risk (OARs). The number of monitor units (MUs) and calculation time are also determined. Good coverage is reported for all algorithm-technique combination plans without exceeding the tolerance for OARs. Regardless of the algorithm, RA plans persistently reported higher D 2% values for PTV-70. All IMRT plans reported higher number of MUs (especially with AXB) than did RA plans. AAA-IMRT produced the minimum calculation time of all plans. Major differences between the investigated algorithm-technique combinations are reported only for the number of MUs and calculation time parameters. In terms of these 2 parameters, it is recommended to employ AXB in calculating RA plans and AAA in calculating IMRT plans to achieve minimum calculation times at reduced number of MUs. Copyright © 2017 American Association of Medical Dosimetrists. Published by Elsevier Inc. All rights reserved.

  2. Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control.

    Science.gov (United States)

    Carroni, Marta; Franke, Kamila B; Maurer, Michael; Jäger, Jasmin; Hantke, Ingo; Gloge, Felix; Linder, Daniela; Gremer, Sebastian; Turgay, Kürşad; Bukau, Bernd; Mogk, Axel

    2017-11-22

    Ring-forming AAA+ chaperones exert ATP-fueled substrate unfolding by threading through a central pore. This activity is potentially harmful requiring mechanisms for tight repression and substrate-specific activation. The AAA+ chaperone ClpC with the peptidase ClpP forms a bacterial protease essential to virulence and stress resistance. The adaptor MecA activates ClpC by targeting substrates and stimulating ClpC ATPase activity. We show how ClpC is repressed in its ground state by determining ClpC cryo-EM structures with and without MecA. ClpC forms large two-helical assemblies that associate via head-to-head contacts between coiled-coil middle domains (MDs). MecA converts this resting state to an active planar ring structure by binding to MD interaction sites. Loss of ClpC repression in MD mutants causes constitutive activation and severe cellular toxicity. These findings unravel an unexpected regulatory concept executed by coiled-coil MDs to tightly control AAA+ chaperone activity.

  3. Analisis Kinerja CV. Fabamus Famili Utama Semarang Dengan Balanced Scorecard

    OpenAIRE

    Permata, Nilla Mega; Nugraha, Hari Susanta; Nurseto, Sendhang

    2014-01-01

    A method of measuring the performance of the company to assist in knowing the health condition of the company itself. The study was conducted on the CV. Fabamus First Family with Balanced Scorecard approach to measuring through four perspectives: financial perspective, customer perspective, internal business process perspective and learning and growth perspectives. In the financial aspects of performance measurement with a weight of 70%, 10% customer perspective, internal business process per...

  4. STRATEGI PEMASARAN PRODUK DIGITAL PRINTING PADA CV. FNB DIGITAL JAMBI

    OpenAIRE

    Yuniarti, Yenni; Mauliana, Sarah

    2012-01-01

    CV. Digital FNB is one of the companies that involved in Digital Printing business. Based on survey in the middle of 2011-2012, there are 31 digital printing businesses in the city of Jambi. In order to examine the marketing stratey implemented, descriptive study has been conducted, uses interview, observation, and documentation as instruments. The strategy that observed in this study consist of marketing strategy including market segmentation, targeting, product strategy, pricing strategy, ...

  5. Applications in Foreign Currency Prima CV Ellindo Money Changer

    OpenAIRE

    Wisnu Sutrisno; Ire Puspa Wardhani, Skom, MMSI

    2004-01-01

    This explains the scientific writing about the design of application programs forforeign currency transactions by using Visual Basic 6.0 programming language.In scientific writing database is also used by Visual Data Manager (VisData)integrated in Visual Basic 6.0 program itself. Consists of three tables namelyCurrency,Customer and Transaction.In the end application program for foreign currency transactions will be applied to theCV. Prima Ellindo Money Changer for ease in performing activitie...

  6. The AAA+ ATPase TRIP13 remodels HORMA domains through N-terminal engagement and unfolding

    Energy Technology Data Exchange (ETDEWEB)

    Ye, Qiaozhen; Kim, Dong Hyun; Dereli, Ihsan; Rosenberg, Scott C.; Hagemann, Goetz; Herzog, Franz; Tóth, Attila; Cleveland, Don W.; Corbett, Kevin D.

    2017-06-28

    Proteins of the conserved HORMA domain family, including the spindle assembly checkpoint protein MAD2 and the meiotic HORMADs, assemble into signaling complexes by binding short peptides termed “closure motifs”. The AAA+ ATPase TRIP13 regulates both MAD2 and meiotic HORMADs by disassembling these HORMA domain–closure motif complexes, but its mechanisms of substrate recognition and remodeling are unknown. Here, we combine X-ray crystallography and crosslinking mass spectrometry to outline how TRIP13 recognizes MAD2 with the help of the adapter protein p31comet. We show that p31comet binding to the TRIP13 N-terminal domain positions the disordered MAD2 N-terminus for engagement by the TRIP13 “pore loops”, which then unfold MAD2 in the presence of ATP. N-terminal truncation of MAD2 renders it refractory to TRIP13 action in vitro, and in cells causes spindle assembly checkpoint defects consistent with loss of TRIP13 function. Similar truncation of HORMAD1 in mouse spermatocytes compromises its TRIP13-mediated removal from meiotic chromosomes, highlighting a conserved mechanism for recognition and disassembly of HORMA domain–closure motif complexes by TRIP13.

  7. AAA-ATPase FIDGETIN-LIKE 1 and Helicase FANCM Antagonize Meiotic Crossovers by Distinct Mechanisms.

    Directory of Open Access Journals (Sweden)

    Chloe Girard

    2015-07-01

    Full Text Available Meiotic crossovers (COs generate genetic diversity and are critical for the correct completion of meiosis in most species. Their occurrence is tightly constrained but the mechanisms underlying this limitation remain poorly understood. Here we identified the conserved AAA-ATPase FIDGETIN-LIKE-1 (FIGL1 as a negative regulator of meiotic CO formation. We show that Arabidopsis FIGL1 limits CO formation genome-wide, that FIGL1 controls dynamics of the two conserved recombinases DMC1 and RAD51 and that FIGL1 hinders the interaction between homologous chromosomes, suggesting that FIGL1 counteracts DMC1/RAD51-mediated inter-homologue strand invasion to limit CO formation. Further, depleting both FIGL1 and the previously identified anti-CO helicase FANCM synergistically increases crossover frequency. Additionally, we showed that the effect of mutating FANCM on recombination is much lower in F1 hybrids contrasting from the phenotype of inbred lines, while figl1 mutation equally increases crossovers in both contexts. This shows that the modes of action of FIGL1 and FANCM are differently affected by genomic contexts. We propose that FIGL1 and FANCM represent two successive barriers to CO formation, one limiting strand invasion, the other disassembling D-loops to promote SDSA, which when both lifted, leads to a large increase of crossovers, without impairing meiotic progression.

  8. La Grande: volver a empezar

    Directory of Open Access Journals (Sweden)

    Julio Premat

    2011-07-01

    Full Text Available Este texto, borrador de un trabajo más amplio, pretende despejar algunas pistas de lectura de La grande, en tanto que paradójico final de la producción de Saer. Digo “paradójico” porque puede tomársela como una novela de comienzo o de origen : de un volver a empezar, en todos los sentidos del término. En esa perspectiva podrían estudiarse algunos núcleos temáticos (como el retorno o el recuerdo de cara a la construcción del texto, a la relación planteada con la tradición y a la singular historia de su escritura (y al material genético que rodea y completa esta novela a la vez inacabada y póstuma. En esta intervención, la idea es la de comentar tres textos, escenas o frases del texto, y a partir de allí esbozar pistas para un estudio que está en ciernes.Première ébauche d’un travail de plus d’ampleur, ce texte vise à éclairer quelques pistes de lecture de La grande, en tant que fin paradoxale de la production de Saer. Je dis « paradoxale » parce que l’on peut considérer cette œuvre comme un roman des commencements ou des origines : comme un retour aux débuts, dans tous les sens du terme. Dans cette perspective, plusieurs nœuds thématiques (comme le retour ou le souvenir pourraient être étudiés en relation à la construction du texte, à la relation qui s’établit avec la tradition et à la singulière histoire de son écriture (et au matériau génétique qui entoure et complète ce roman, à la fois inachevé et posthume. Il sera question ici de commenter trois textes, scènes ou phrases du texte, et d’ébaucher à partir de là quelques pistes pour une étude à l’état naissant.The objective of this text, a draft for a broader work, is to outline some reading clues for La grande, inasmuch as it constitutes a paradoxical ending of Saer’s production. I say “paradoxical” because we can consider this work a novel of beginnings or of origins: a return to the beginning, in every meaning of the

  9. The draft genome of Corchorus olitorius cv. JRO-524 (Navin

    Directory of Open Access Journals (Sweden)

    Debabrata Sarkar

    2017-06-01

    Full Text Available Here, we present the draft genome (377.3 Mbp of Corchorus olitorious cv. JRO-524 (Navin, which is a leading dark jute variety developed from a cross between African (cv. Sudan Green and indigenous (cv. JRO-632 types. We predicted from the draft genome a total of 57,087 protein-coding genes with annotated functions. We identified a large number of 1765 disease resistance-like and defense response genes in the jute genome. The annotated genes showed the highest sequence similarities with that of Theobroma cacao followed by Gossypium raimondii. Seven chromosome-scale genetically anchored pseudomolecules were constructed with a total size of 8.53 Mbp and used for synteny analyses with the cocoa and cotton genomes. Like other plant species, gypsy and copia retrotransposons were the most abundant classes of repeat elements in jute. The raw data of our study are available in SRA database of NCBI with accession number SRX1506532. The genome sequence has been deposited at DDBJ/EMBL/GenBank under the accession LLWS00000000, and the version described in this paper will be the first version (LLWS01000000.

  10. Height, leaf nymber, chemical composition and dry matter production of Stylosanthes Campo Grande at different levels of potassium and zinco.

    Directory of Open Access Journals (Sweden)

    Françoise Mara Gomes

    2015-10-01

    Full Text Available The objective of this study was to determine plant height, total number of leaves, number of live leaves, chemical composition and dry mass production of Stylosanthes cv. Campo Grande at first cut and after 21 days of regrowth at different levels of potassium (K2O with and without zinc (Zn. The experiment was conducted using a randomized block design in a 4 x 2 factorial scheme consisting of four repetitions. Four levels of K2O (0, 120, 240 and 360 mg/dm3 with and without Zn (0 and 6 mg/dm3 were used. There was no effect of the interaction between K2O and Zn levels on the structural characteristics of Stylosanthes cv. Campo Grande, and no independent effects of the different levels of K2O and Zn were observed. The mean plant height, total number of leaves and number of live leaves were 21.2 cm, 30.2 and 27.2, respectively. Dry mass production did not differ between K2O and Zn levels, with a mean production of 3.7 g/pot. There was also no effect of the interaction between K2O and Zn levels on dry matter and neutral detergent fiber content, and no independent effects of the different levels of K2O and Zn were observed, with mean values of 29.3% and 46.9% dry matter, respectively. However, an effect of the interaction between K2O and Zn levels was observed for crude protein content, which exhibited a quadratic response. Re2growth increased linearly with increasing K2O levels. Although the highest crude protein content was obtained at zero levels of potassium and zinc, potassium fertilization is advantageous since it increases the regrowth of Stylosanthes cv. Campo Grande in 21 days.

  11. A Grande Reportagem no contexto informativo SIC

    OpenAIRE

    Colaço, Vanessa Alexandra Francisco

    2014-01-01

    Os telespectadores querem ver grandes reportagens? Como evoluíram as audiências da Grande Reportagem SIC? É este o produto premium da estação? Terá este formato um investimento e continuidade garantidas? Estas são algumas das questões formuladas e às quais se procurou dar resposta neste Relatório de Estágio. Neste trabalho traça-se o perfil do programa Grande Reportagem SIC, clarificando a linha editorial que lhe serviu de base, procurando perceber as suas dinâmicas e passando em revista mome...

  12. Supersymmetry and supergravity: Phenomenology and grand unification

    International Nuclear Information System (INIS)

    Arnowitt, R.; Nath, P.

    1993-01-01

    A survey is given of supersymmetry and supergravity and their phenomenology. Some of the topics discussed are the basic ideas of global supersymmetry, the minimal supersymmetric Standard Model (MSSM) and its phenomenology, the basic ideas of local supersymmetry (supergravity), grand unification, supersymmetry breaking in supergravity grand unified models, radiative breaking of SU(2) x U(1), proton decay, cosmological constraints, and predictions of supergravity grand unified models. While the number of detailed derivations are necessarily limited, a sufficient number of results are given so that a reader can get a working knowledge of this field

  13. Functional representation for the grand partition function of a multicomponent system of charged particles: Correlation functions of the reference system

    Directory of Open Access Journals (Sweden)

    O.V.Patsahan

    2006-01-01

    Full Text Available Based on the method of collective variables (CV with a reference system, the exact expression for the functional of the grand partition function of a m-component ionic model with charge and size asymmetry is found. Particular attention is paid to the n-th particle correlation functions of the reference system which is presented as a m-component system of "colour" hard spheres of the same diameter. A two-component model is considered in more detail. In this case the recurrence formulas for the correlation functions are found. A general case of a m-component inhomogeneous system of the "colour" hard spheres is also analysed.

  14. Analisis Daya Saing untuk Meningkatkan Kualitas Layanan terhadap Pelanggan dengan Pendekatan CRM di CV. BLITZSPOT

    Directory of Open Access Journals (Sweden)

    Suryadi Muhammad

    2017-04-01

    Full Text Available CV. Blitzspot adalah perusahaan yang bergerak di bidang jasa penyediaan layanan internet (internet service provider, yang berkantor pusat di Komplek Padasuka Indah Ruko Blok A-1, Cimahi, Jawa Barat. Berdasarkan hasil wawancara dan hasil observasi pada data transaksi CV. Blitzspot, konsumen CV. Blitzspot yang melakukan transaksi tiap tahunnya selalu berkurang, hal ini dikarenakan konsumen sering membandingkan layanan-layanan yang ditawarkan oleh para pesaing CV. Blitzspot. Bila permasalahan tersebut belum teratasi, konsumen CV. Blitzspot akan menurun tiap tahunnya dan akan selalu berpindah ke pesaing CV. Blitzspot. Direktur Utama CV. Blitzspot ingin menganalisis daya saing untuk menentukan layanan-layanan yang akan diberikan kepada pelanggan CV. Blitzspot dan penentuan strategi untuk mempertahankan konsumen CV. Blitzspot.  Metode Analisis daya saing yang digunakan adalah Model Analisis Berlian Porter dan Metode Pengelompokkan Pelanggan yang digunakan adalah Metode LRFM. Berdasarkan hasil pengujian dan pembahasan yang telah dibuat, maka dapat ditarik kesimpulan bahwa Direktur Utama dapat menganalisis daya saing CV. Blitzspot dengan menggunakan Berlian Porter untuk meningkatkan kualitas layanan terhadap pelanggan CV. Blitzspot.

  15. Las cinco grandes dimensiones de la personalidad

    Directory of Open Access Journals (Sweden)

    Jan ter Laak

    1996-12-01

    Full Text Available Este artículo revisa las distintas posiciones teóricas sobre las cinco grandes dimensiones de la personalidad, mostrando las semejanzas y diferencias entre las posturas teóricas. Esta contribución presenta lo siguiente: (a la génesis del contenido y la estructura de las cinco dimensiones; (b la fortaleza de las cinco dimensiones; (e la relación de las cinco grandes dimensiones con otros constructos de personalidad; (d discute el valor predictivo de las puntuaciones del perfil de las cinco dimensiones para criterios pertinentes; (e analiza el estatus teórico de las cinco dimensiones; (f discute críticas históricas sobre las cinco grandes dimensiones y se formulan respuestas a estas críticas; (g hace conjeturas para el futuro de las cinco grandes dimensiones; y (h concluye con algunas conclusiones y comentarios.

  16. Produção integrada e convencional de pêssegos cv. Marli Integrated and convetional production of peach cv. Marli

    Directory of Open Access Journals (Sweden)

    José Luis da Silva Nunes

    2004-12-01

    Full Text Available O cultivo de pessegueiros é uma atividade de grande importância econômica no Sul do Brasil, onde se destaca o Estado do Rio Grande do Sul como maior produtor brasileiro. Um dos aspectos mais importantes na produção de alimentos da atualidade é a redução no uso de agroquímicos, com menor contaminação do ambiente e riscos reduzidos de resíduos. Este trabalho visou a comparar os sistemas de Produção Convencional (PC e Integrada (PI de pêssegos e foi realizado no ano de 2001, no município de São Jerônimo - RS, latitude 30°05'52" S, longitude 51°39'08" W e altitude de 46 metros. Áreas de um pomar comercial da cv. Marli foram avaliadas em relação às principais práticas de manejo da planta e do solo, controle fitossanitário, aspectos econômicos, bem como à qualidade da fruta. Na área conduzida sob PI, foram utilizadas as práticas de manejo preconizadas pelas Normas de Produção Integrada de Pêssegos (NPIP e, na área conduzida no sistema de PC, as plantas foram manejadas de acordo com as práticas comumente utilizadas pelo produtor. A produção de pêssegos, em ambos os sistemas, não foi afetada. Na área de PI, houve menor número de pêssegos por planta; entretanto, as frutas apresentaram maior peso médio. A maioria dos pêssegos da PI foram classificados como CAT I (diâmetro superior a 57 mm. As frutas produzidas na PC são, na maioria, de CAT II (de 48 a 57 mm. A qualidade pós-colheita não apresentou diferenças em relação à acidez, firmeza e cor. Com base nestes resultados, podemos concluir que é possível produzir pêssegos de qualidade com produtividade no sistema de PI.The peach-trees cultivation is a very economic important activity in South of Brazil, where highlights the State of Rio Grande do Sul as the largest Brazilian producer. One of the most important aspects in actuality is the food prodution with reduction of agrochemicals components, reducing the risks of environment contamination. This work

  17. Grand unification and gravity - selected topics

    International Nuclear Information System (INIS)

    Zee, A.

    1981-09-01

    The material given here was presented in lectures delivered at the 4th Kyoto Summer Institute on Grand Unification and Related Topics. It consists of six sections. The sections are: the family problem, fermion mass hierarchy, maximal local symmetry, operator analysis of new physics, dynamically generated gravity, and Kaluza theory and grand unification. The last section contains a (hopefully) pedagogical introduction to Kaluza theory. For pedagogical completeness, several appendices reviewing some elementary notions of differential geometry have been added

  18. Histochemical response of `Grande naine' plants inoculated with M. fijiensis and Bacillus pumilus CCIBP-C5 cell free filtrate

    Directory of Open Access Journals (Sweden)

    Eilyn Mena

    2015-04-01

    Full Text Available In recent years, interest has won biocontrol Mycosphaerella fijiensis with the use of microorganisms or their products. Nevertheless, the knowledge of the biochemical events involved in Musa- M. fijiensis-microorganism interaction, is still limited. The objective was to determine the histochemical response of `Grande naine' (Musa AAA plants inoculated or not with the pathogen in the presence of culture filtrate of Bacillus pumilus CCIBP-C5. For this, techniques that allow visualization of callose, superoxide anion, lignin and phenolic compounds in the early days post inoculation (dpi were used. As a result, at 6 dpi callose deposition were observed within the stomata in plants where the culture filtrate was applied regardless of the presence or absence of the pathogen. Furthermore, a blue halo was observed around stomas, indicative of the presence of superoxide anion. It was not possible to detect accumulation of lignin or phenolic compounds with the technique used. It was demonstrated the accumulation of biochemical compounds related to defense response of `Grande naine' plants to pathogen infection. Furthermore, it was shown that the plant responds in early infection with the use of bacterial culture filtrates. These results demonstrate the possible mechanism of induction of defense response in plants of Musa sp. in the presence of bacterial culture filtrate. Key words: Black Sigatoka, callose, lignin, phenols, superoxide anion

  19. Phenotype–genotype spectrum of AAA syndrome from Western India and systematic review of literature

    Directory of Open Access Journals (Sweden)

    Hiren Patt

    2017-11-01

    Full Text Available Objective: To study genotype–phenotype spectrum of triple A syndrome (TAS. Methods: Retrospective chart analysis of Indian TAS patients (cohort 1, n = 8 and review of genotyped TAS cases reported in world literature (cohort 2, n = 133, 68 publications. Results: Median age at presentation was 4.75 years (range: 4–10 and 5 years (range: 1–42 for cohorts 1 and 2, respectively. Alacrima, adrenal insufficiency (AI, achalasia and neurological dysfunction (ND were seen in 8/8, 8/8, 7/8 and 4/8 patients in cohort 1, and in 99, 91, 93 and 79% patients in cohort 2, respectively. In both cohorts, alacrima was present since birth while AI and achalasia manifested before ND. Mineralocorticoid deficiency (MC was uncommon (absent in cohort 1, 12.5% in cohort 2. In cohort 1, splice-site mutation in exon 1 (p.G14Vfs*45 was commonest, followed by a deletion in exon 8 (p.S255Vfs*36. Out of 65 mutations in cohort 2, 14 were recurrent and five exhibited regional clustering. AI was more prevalent, more often a presenting feature, and was diagnosed at younger age in T group (those with truncating mutations as compared to NT (non-truncating mutations group. ND was more prevalent, more common a presenting feature, with later age at onset in NT as compared to T group. Conclusion: Clinical profile of our patients is similar to that of patients worldwide. Alacrima is the earliest and most consistent finding. MC deficiency is uncommon. Some recurrent mutations show regional clustering. p.G14Vfs*45 and p.S255Vfs*36 account for majority of AAAS mutations in our cohort. Phenotype of T group differs from that of NT group and merits future research.

  20. EFECTO DE DOS TIPOS DE FUNDAS SOBRE EL FRUTO DE BANANO (Musa AAA

    Directory of Open Access Journals (Sweden)

    Alfonso Vargas-Calvo

    2011-01-01

    Full Text Available El objetivo de este estudio fue determinar el efecto de dos fundas en la protección del racimo de banano (Musa AAA . En dos épocas climáticas (adversa y favorable bajo condiciones del Ca ribe de Costa Ri ca se evaluaron dos fundas: 1- azul Sa nta Lucía (bifentrina 0,1%, polie tileno de 12,7 ¿ de grosor, con perforaciones de 4 mm y 86,4 cm de ancho y 2- transparente con aditivos para filtrar la luz ultravioleta e infrarroja (bifentrina 0,1%, 20,3 ¿ de grosor, con perforaciones de 4 mm y 88,9 cm de ancho. El peso del racimo así como el grosor y la longitud del fruto central de la fila externa en la segunda, cuarta y sexta mano no difirieron (P> 0,0556 entre ambas fundas. Tampoco hubo diferencias entre estas en la apa rie ncia del racimo (P>0,4699 ni en la firmeza de la cáscara en grado 1 de maduración (P= 0,6268. En las varia bles de medición del color del fruto solamente L* presentó un valor (56,48 más alto (P=0,0109 con la funda transparente fotosensible, mie ntras que las otras dos varia bles relacionadas (a* y b* no fueron diferentes (P>0,1011 entre las fundas. El incremento adicional de 7,6 ¿ en el grosor del polie tileno de la funda transparente con respecto a la azul Sa nta Lucía, no ocasionó un incremento productivo ni una mejor apa rie ncia del racimo de banano y sus frutos.

  1. Design of experiments in medical physics: Application to the AAA beam model validation.

    Science.gov (United States)

    Dufreneix, S; Legrand, C; Di Bartolo, C; Bremaud, M; Mesgouez, J; Tiplica, T; Autret, D

    2017-09-01

    The purpose of this study is to evaluate the usefulness of the design of experiments in the analysis of multiparametric problems related to the quality assurance in radiotherapy. The main motivation is to use this statistical method to optimize the quality assurance processes in the validation of beam models. Considering the Varian Eclipse system, eight parameters with several levels were selected: energy, MLC, depth, X, Y 1 and Y 2 jaw dimensions, wedge and wedge jaw. A Taguchi table was used to define 72 validation tests. Measurements were conducted in water using a CC04 on a TrueBeam STx, a TrueBeam Tx, a Trilogy and a 2300IX accelerator matched by the vendor. Dose was computed using the AAA algorithm. The same raw data was used for all accelerators during the beam modelling. The mean difference between computed and measured doses was 0.1±0.5% for all beams and all accelerators with a maximum difference of 2.4% (under the 3% tolerance level). For all beams, the measured doses were within 0.6% for all accelerators. The energy was found to be an influencing parameter but the deviations observed were smaller than 1% and not considered clinically significant. Designs of experiment can help define the optimal measurement set to validate a beam model. The proposed method can be used to identify the prognostic factors of dose accuracy. The beam models were validated for the 4 accelerators which were found dosimetrically equivalent even though the accelerator characteristics differ. Copyright © 2017 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

  2. Phenotype–genotype spectrum of AAA syndrome from Western India and systematic review of literature

    Science.gov (United States)

    Patt, Hiren; Koehler, Katrin; Lodha, Sailesh; Yerawar, Chaitanya; Huebner, Angela; Thakkar, Kunal; Arya, Sneha; Nair, Sandhya; Goroshi, Manjunath; Ganesh, Hosahithlu; Sarathi, Vijaya; Lila, Anurag; Bandgar, Tushar; Shah, Nalini

    2017-01-01

    Objective To study genotype–phenotype spectrum of triple A syndrome (TAS). Methods Retrospective chart analysis of Indian TAS patients (cohort 1, n = 8) and review of genotyped TAS cases reported in world literature (cohort 2, n = 133, 68 publications). Results Median age at presentation was 4.75 years (range: 4–10) and 5 years (range: 1–42) for cohorts 1 and 2, respectively. Alacrima, adrenal insufficiency (AI), achalasia and neurological dysfunction (ND) were seen in 8/8, 8/8, 7/8 and 4/8 patients in cohort 1, and in 99, 91, 93 and 79% patients in cohort 2, respectively. In both cohorts, alacrima was present since birth while AI and achalasia manifested before ND. Mineralocorticoid deficiency (MC) was uncommon (absent in cohort 1, 12.5% in cohort 2). In cohort 1, splice-site mutation in exon 1 (p.G14Vfs*45) was commonest, followed by a deletion in exon 8 (p.S255Vfs*36). Out of 65 mutations in cohort 2, 14 were recurrent and five exhibited regional clustering. AI was more prevalent, more often a presenting feature, and was diagnosed at younger age in T group (those with truncating mutations) as compared to NT (non-truncating mutations) group. ND was more prevalent, more common a presenting feature, with later age at onset in NT as compared to T group. Conclusion Clinical profile of our patients is similar to that of patients worldwide. Alacrima is the earliest and most consistent finding. MC deficiency is uncommon. Some recurrent mutations show regional clustering. p.G14Vfs*45 and p.S255Vfs*36 account for majority of AAAS mutations in our cohort. Phenotype of T group differs from that of NT group and merits future research. PMID:29180348

  3. Induction of single flower mutant in Hibiscus cv. 'Alipur Beauty'

    International Nuclear Information System (INIS)

    Banerji, B.K.; Datta, S.K.

    1986-01-01

    Stem cuttings of Hibicus cv. 'Alipur Beauty' were irradiated with 0, 1.0, 2.0 Krad of gamma rays in 60 Co source and planted just after irradiation. Data were recorded on days to sprout, number of sprouts, sprouting percentage, survival, plant height, leaf number and size, abnormal leaves and plants, floral abnormalities and induction of somatic mutations in flower colour/form. Some of the characters like plant height, leaf number, abnormal leaves and plants and leaf size were recorded after 60 and 120 days. 7 refs. (author)

  4. DETECTION OF MOTORCYCLISTS WITHOUT HELMET AND FINEPAYMENT USING OPEN CV

    OpenAIRE

    Stemy Simon, Divya Kumaran A.K.

    2018-01-01

    The helmet is the main safety equipment of motorcyclists, but many drivers do not use it. The main aim of this project is to construct an automatic detection of the motorcyclist without helmet from video using OpenCV library tools. If they are not wearing the helmet, the license plate of the motorcycle is focused automatically. By using Computer Vision technique we can detect and recognize the license plate number. We make the training set of different characters of different sizes. Based on ...

  5. Comparison of tracking algorithms implemented in OpenCV

    Directory of Open Access Journals (Sweden)

    Janku Peter

    2016-01-01

    Full Text Available Computer vision is very progressive and modern part of computer science. From scientific point of view, theoretical aspects of computer vision algorithms prevail in many papers and publications. The underlying theory is really important, but on the other hand, the final implementation of an algorithm significantly affects its performance and robustness. For this reason, this paper tries to compare real implementation of tracking algorithms (one part of computer vision problem, which can be found in the very popular library OpenCV. Moreover, the possibilities of optimizations are discussed.

  6. OpenCV 3.0 computer vision with Java

    CERN Document Server

    Baggio, Daniel Lélis

    2015-01-01

    If you are a Java developer, student, researcher, or hobbyist wanting to create computer vision applications in Java then this book is for you. If you are an experienced C/C++ developer who is used to working with OpenCV, you will also find this book very useful for migrating your applications to Java. All you need is basic knowledge of Java, with no prior understanding of computer vision required, as this book will give you clear explanations and examples of the basics.

  7. AVALIAÇÃO DA CV. CABERNET FRANC PARA ELABORAÇÃO DE VINHO TINTO EVALUATION OF CV. CABERNET FRANC TO ELABORATE RED WINE

    Directory of Open Access Journals (Sweden)

    Luiz Antenor RIZZON

    2001-08-01

    Full Text Available A uva Cabernet Franc, originária da região de Bordeaux, França, foi introduzida no Rio Grande do Sul através da Estação Agronômica de Porto Alegre. É utilizada para a elaboração de vinho tinto para ser consumido jovem, embora apresente aptidão para envelhecer. Face a sua importância, conduziu-se este trabalho com o objetivo de determinar as características agronômicas e enológicas da uva Cabernet Franc para elaboração de vinho tinto. Para isso, realizaram-se estudos para caracterizar o cacho, o mosto e o vinho tinto nas safras de 1987 a 1994. Os resultados obtidos evidenciaram que a cv. Cabernet Franc tem cacho médio, formado por bagas pequenas. O mosto possui teores de açúcar e de acidez adequados para vinificação. O vinho apresenta elevado teor de K e de álcoois superiores. No aspecto sensorial, apresenta cor vermelho-rubi, com reflexos violáceos quando jovem, e de intensidade variável em função das safras vitícolas. No olfato, apresenta aroma com notas vegetais e frutadas, que lembram pimentão e frutas vermelhas, respectivamente. Gustativamente, o vinho apresenta boa estrutura, equilíbrio e personalidade marcante.Cabernet Franc grape is originated from Bordeaux, France. It was introduced in the Serra Gaúcha region in the south of Brazil, by the Estação Agronômica the Porto Alegre, RS. It is used to elaborate young red wine, but it can be aged too. Due to the importance of Cabernet Franc, this work had the objective to determine its agronomic and winemaking characteristics. To achieve this purpose, variables related to the berries, clusters, must, and wine were performed from 1987 to 1994. The results showed that Cabernet Franc grapes have clusters of medium size and small berries. The must has adequate sugar and titratable acidity levels for vinification. The Cabernet Franc wine has a high concentration of K and higher alcohols. The sensory evaluation showed that it has a ruby color with violet reflexes when

  8. Accuracy of Acuros XB and AAA dose calculation for small fields with reference to RapidArc stereotactic treatments

    International Nuclear Information System (INIS)

    Fogliata, Antonella; Nicolini, Giorgia; Clivio, Alessandro; Vanetti, Eugenio; Cozzi, Luca

    2011-01-01

    Purpose: To assess the accuracy against measurements of two photon dose calculation algorithms (Acuros XB and the Anisotropic Analytical algorithm AAA) for small fields usable in stereotactic treatments with particular focus on RapidArc. Methods: Acuros XB and AAA were configured for stereotactic use. Baseline accuracy was assessed on small jaw-collimated open fields for different values for the spot sizes parameter in the beam data: 0.0, 0.5, 1, and 2 mm. Data were calculated with a grid of 1 x 1 mm 2 . Investigated fields were: 3 x 3, 2 x 2, 1 x 1, and 0.8 x 0.8 cm 2 with a 6 MV photon beam generated from a Clinac2100iX (Varian, Palo Alto, CA). Profiles, PDD, and output factors were measured in water with a PTW diamond detector (detector size: 4 mm 2 , thickness 0.4 mm) and compared to calculations. Four RapidArc test plans were optimized, calculated and delivered with jaw settings J3 x 3, J2 x 2, and J1 x 1 cm 2 , the last was optimized twice to generate high (H) and low (L) modulation patterns. Each plan consisted of one partial arc (gantry 110 deg. to 250 deg.), and collimator 45 deg. Dose to isocenter was measured in a PTW Octavius phantom and compared to calculations. 2D measurements were performed by means of portal dosimetry with the GLAaS method developed at authors' institute. Analysis was performed with gamma pass-fail test with 3% dose difference and 2 mm distance to agreement thresholds. Results: Open square fields: penumbrae from open field profiles were in good agreement with diamond measurements for 1 mm spot size setting for Acuros XB, and between 0.5 and 1 mm for AAA. Maximum MU difference between calculations and measurements was 1.7% for Acuros XB (0.2% for fields greater than 1 x 1 cm 2 ) with 0.5 or 1 mm spot size. Agreement for AAA was within 0.7% (2.8%) for 0.5 (1 mm) spot size. RapidArc plans: doses were evaluated in a 4 mm diameter structure at isocenter and computed values differed from measurements by 0.0, -0.2, 5.5, and -3.4% for

  9. Accuracy of Acuros XB and AAA dose calculation for small fields with reference to RapidArc stereotactic treatments

    Energy Technology Data Exchange (ETDEWEB)

    Fogliata, Antonella; Nicolini, Giorgia; Clivio, Alessandro; Vanetti, Eugenio; Cozzi, Luca [Oncology Institute of Southern Switzerland, Medical Physics Unit, CH-6500 Bellinzona (Switzerland)

    2011-11-15

    Purpose: To assess the accuracy against measurements of two photon dose calculation algorithms (Acuros XB and the Anisotropic Analytical algorithm AAA) for small fields usable in stereotactic treatments with particular focus on RapidArc. Methods: Acuros XB and AAA were configured for stereotactic use. Baseline accuracy was assessed on small jaw-collimated open fields for different values for the spot sizes parameter in the beam data: 0.0, 0.5, 1, and 2 mm. Data were calculated with a grid of 1 x 1 mm{sup 2}. Investigated fields were: 3 x 3, 2 x 2, 1 x 1, and 0.8 x 0.8 cm{sup 2} with a 6 MV photon beam generated from a Clinac2100iX (Varian, Palo Alto, CA). Profiles, PDD, and output factors were measured in water with a PTW diamond detector (detector size: 4 mm{sup 2}, thickness 0.4 mm) and compared to calculations. Four RapidArc test plans were optimized, calculated and delivered with jaw settings J3 x 3, J2 x 2, and J1 x 1 cm{sup 2}, the last was optimized twice to generate high (H) and low (L) modulation patterns. Each plan consisted of one partial arc (gantry 110 deg. to 250 deg.), and collimator 45 deg. Dose to isocenter was measured in a PTW Octavius phantom and compared to calculations. 2D measurements were performed by means of portal dosimetry with the GLAaS method developed at authors' institute. Analysis was performed with gamma pass-fail test with 3% dose difference and 2 mm distance to agreement thresholds. Results: Open square fields: penumbrae from open field profiles were in good agreement with diamond measurements for 1 mm spot size setting for Acuros XB, and between 0.5 and 1 mm for AAA. Maximum MU difference between calculations and measurements was 1.7% for Acuros XB (0.2% for fields greater than 1 x 1 cm{sup 2}) with 0.5 or 1 mm spot size. Agreement for AAA was within 0.7% (2.8%) for 0.5 (1 mm) spot size. RapidArc plans: doses were evaluated in a 4 mm diameter structure at isocenter and computed values differed from measurements by 0.0, -0

  10. Evaluation of the dose calculation accuracy for small fields defined by jaw or MLC for AAA and Acuros XB algorithms.

    Science.gov (United States)

    Fogliata, Antonella; Lobefalo, Francesca; Reggiori, Giacomo; Stravato, Antonella; Tomatis, Stefano; Scorsetti, Marta; Cozzi, Luca

    2016-10-01

    Small field measurements are challenging, due to the physical characteristics coming from the lack of charged particle equilibrium, the partial occlusion of the finite radiation source, and to the detector response. These characteristics can be modeled in the dose calculations in the treatment planning systems. Aim of the present work is to evaluate the MU calculation accuracy for small fields, defined by jaw or MLC, for anisotropic analytical algorithm (AAA) and Acuros XB algorithms, relative to output measurements on the beam central axis. Single point output factor measurement was acquired with a PTW microDiamond detector for 6 MV, 6 and 10 MV unflattened beams generated by a Varian TrueBeam STx equipped with high definition-MLC. Fields defined by jaw or MLC apertures were set; jaw-defined: 0.6 × 0.6, 0.8 × 0.8, 1 × 1, 2 × 2, 3 × 3, 4 × 4, 5 × 5, and 10 × 10 cm 2 ; MLC-defined: 0.5 × 0.5 cm 2 to the maximum field defined by the jaw, with 0.5 cm stepping, and jaws set to: 2 × 2, 3 × 3, 4 × 4, 5 × 5, and 10 × 10 cm 2 . MU calculation was obtained with 1 mm grid in a virtual water phantom for the same fields, for AAA and Acuros algorithms implemented in the Varian eclipse treatment planning system (version 13.6). Configuration parameters as the effective spot size (ESS) and the dosimetric leaf gap (DLG) were varied to find the best parameter setting. Differences between calculated and measured doses were analyzed. Agreement better than 0.5% was found for field sizes equal to or larger than 2 × 2 cm 2 for both algorithms. A dose overestimation was present for smaller jaw-defined fields, with the best agreement, averaged over all the energies, of 1.6% and 4.6% for a 1 × 1 cm 2 field calculated by AAA and Acuros, respectively, for a configuration with ESS = 1 mm for both X and Y directions for AAA, and ESS = 1.5 and 0 mm for X and Y directions for Acuros. Conversely, a calculated dose underestimation was found for small MLC-defined fields, with the

  11. m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation.

    Science.gov (United States)

    Kolodziejczak, Marta; Skibior-Blaszczyk, Renata; Janska, Hanna

    2018-05-01

    For optimal mitochondrial activity, the mitochondrial proteome must be properly maintained or altered in response to developmental and environmental stimuli. Based on studies of yeast and humans, one of the key players in this control are m-AAA proteases, mitochondrial inner membrane-bound ATP-dependent metalloenzymes. This study focuses on the importance of m-AAA proteases in plant mitochondria, providing their first experimentally proven physiological substrate. We found that the Arabidopsis m- AAA complexes composed of AtFTSH3 and/or AtFTSH10 are involved in the proteolytic maturation of ribosomal subunit L32. Consequently, in the double Arabidopsis ftsh3/10 mutant, mitoribosome biogenesis, mitochondrial translation and functionality of OXPHOS (oxidative phosphorylation) complexes are impaired. However, in contrast to their mammalian or yeast counterparts, plant m-AAA complexes are not critical for the survival of Arabidopsis under optimal conditions; ftsh3/10 plants are only slightly smaller in size at the early developmental stage compared with plants containing m-AAA complexes. Our data suggest that a lack of significant visible morphological alterations under optimal growth conditions involves mechanisms which rely on existing functional redundancy and induced functional compensation in Arabidopsis mitochondria.

  12. Alcohol and CV Health: Jekyll and Hyde J-Curves.

    Science.gov (United States)

    O'Keefe, Evan L; DiNicolantonio, James J; O'Keefe, James H; Lavie, Carl J

    2018-02-16

    A routine of light or moderate alcohol consumption (≤1 drink/day for women and 1 to 2 drinks/day for men) is associated with a lower risk for all-cause mortality, coronary artery disease (CAD), type 2 diabetes mellitus (T2D), heart failure (HF), and stroke. Conversely, heavy drinking, (>4 drinks/day) is associated with an increased risk for death and cardiovascular (CV) disease (CVD). Excessive alcohol intake trails behind only smoking and obesity among the 3 leading causes of premature deaths in the United States (US). Heavy alcohol use is a common cause of reversible hypertension (HTN), nonischemic dilated cardiomyopathy, atrial fibrillation (AF), and stroke (both ischemic and hemorrhagic). Among males aged 15 to 59 years, alcohol abuse is perhaps the leading cause of premature death. As such, the risk-to-benefit ratio of drinking is less favorable in younger individuals. A daily habit of light to moderate drinking is ideal for those who choose to consume alcohol regularly. Red wine in particular before or during the evening meal is linked with the best long-term CV outcomes. Most of the studies on alcohol and health are observational, and correlation does not prove causation. Health care professionals should not advise nondrinkers to begin drinking because of the paucity of randomized outcome data coupled with the potential for alcohol abuse even among seemingly low risk individuals. Copyright © 2017. Published by Elsevier Inc.

  13. Resistance selection on banana CV. Ambon Kuning Against Fusarium Wilt

    International Nuclear Information System (INIS)

    Sutarto, Ismiyarti; Meldia, Yeni; Jumjunidang

    1998-01-01

    This research was conducted in order to study the occurrence of mutation on irradiated plantlets and their resistance of plants of banana cv. Ambon Kuning against Fusarium wilt. Plantlets of banana cv. Ambon Kuning sized 5 cm were exposed to gamma rays at the doses 5 - 35 Gy intervals, then were subcultured for obtained M 1 V 5 plantlets. More over, the planlets were acclimatized and were planted in the field was already infected by Fasarium (f).culbense (FOC). The result indicated that irradiated plantlets of the doses 20 - 35 Gy were not able to survive up to 6 months after exposing to gamma rays. Abnormalities of M 1 V 5 plantlets originated from irradiated plantlets at the doses 10 and 15 Gy were shown on rossette plantlets with rigid and dark green leaves, and the formation of smooth mass morphologically shaped like calculus. The appearance of plant height and number of suckers of suckers of M 1 V 5 plants in the field was quite various. The number of survival plants after 8 moths planting was 8, 7, 15, and 28, respectively originated from untreated plants and irradiated plantlets at the doses 5, 10, and 15 Gy. After one year planting , only 2 plants were able to survive from irradiated plantlet at the dose 15 Gy. The plants could produce 27 plantlets obtained from culturing their shoot tips. Further study of these plantlets was needed in order create the stability of their resistance to FOC. (author)

  14. EN-CV during LS1: upgrade, consolidation, maintenance, operation

    International Nuclear Information System (INIS)

    Nonis, M.

    2012-01-01

    The Cooling and Ventilation (CV) Group in the Engineering Department (EN) will be heavily involved in several projects and activities during the long shutdown in 2013 and 2014 (LS1) within a time-frame limited to around twelve months. According to the requests received so far, most projects are related to the upgrade of users' equipment, consolidation work, and the construction of new plants. However, through the experience gained from the first years of the LHC run, some projects are also needed to adapt the existing installations to the new operating parameters. Some of these projects are presented hereafter, outlining the impact that they will have on operational working conditions or risks of breakdown. Among these projects we find: the PM32 raising pumps, the cooling of the CERN Control Center, R2E, the backup cooling towers for ATLAS and cryogenics, a thermosyphon for ATLAS, or new pumps in UWs. Finally, EN-CV activities during LS1 for maintenance, operation, and commissioning will be mentioned since they represent a major workload for the Group

  15. Perancangan Kemasan Transportasi Buah Jambu Air (Syzygium aqueum cv Camplong

    Directory of Open Access Journals (Sweden)

    Iswahyudi

    2015-04-01

    Full Text Available Jamboo cv Camplong was an exotic fruit from Sampang Indonesia which had high economic values. The quality of fresh Jamboo was greatly influenced by the types of packaging and ways of its transportation that affected its shelf life. The purpose of this research were designing a primary packaging and analyzing the quality of Jamboo cv Camplong after short transportation (from Sampang to Surabaya. Farmers used conventional packaging with capacity of 8.4 kg/box (dimension 478 mm x 146 mm x 354 mm. Based on theoretical packaging design showed that the flute BC cardboard (capacity 4.5 kg/box, dimension 357 mm x 217 mm x 216 mm with partition flute A cardboard. In fact, there was needed modification of the dimension (342 mm x 210 mm x 200 mm because of the fruits diameter (60-65 mm. This result did not change the efficiency usage of transportation space (91-95% and compression strength of box can support (7 boxes/ stack. The result showed that the mechanical damage after transportation were 20.87% for conventional packaging (as control and 7.70% for modification packaging design (packaging with partition.

  16. The INNOVATION Trial: four-year safety and effectiveness of the INCRAFT® AAA Stent-Graft System for endovascular repair.

    Science.gov (United States)

    Pratesi, Giovanni; Pratesi, Carlo; Chiesa, Roberto; Coppi, Gioacchino; Scheinert, Dierk; Brunkwall, Jan S; van der Meulen, Stefaan; Torsello, Giovanni

    2017-10-01

    This paper reports the 4-year safety and effectiveness of the INCRAFT® AAA Stent-Graft System (Cordis Corp., Milpitas, CA, USA), an ultra-low-profile device for the treatment of abdominal aortic aneurysms. The INNOVATION Trial is the prospective, first-in-human, multicenter trial to evaluate the safety and effectiveness of the INCRAFT® System. Patients underwent annual clinical and computed tomography angiography examination as part of the study protocol. The INCRAFT® AAA Stent-Graft System is a customizable tri-modular design, with an ultra-low profile (14-Fr) delivery system. Patient were treated under approved protocol, the prescribed clinical and imaging follow-up at annually through 5 years. Results analyzed and adjudicated by a clinical events committee, independent core laboratory, and a data safety and monitoring board. This manuscript reports results through 4 years of follow-up. A total of 60 patients were enrolled in the trial, all of whom were successfully treated. Follow-up rates at 1 and 4 years were 93% (56/60) and 85% (51/60), respectively. All-cause mortality at 4 years was 17.6% and no death was AAA-, device-, or procedure-related. The secondary reintervention rate at 1 year was 4.6%, primarily the result of stent thrombosis. In total, 10 patients required 13 post-procedure interventions within 4-years of follow-up (2 to repair a type I endoleak, 4 to repair a type II endoleak, 1 for stent thrombosis, 1 for renal stenosis, 1 for aneurysm enlargement, 2 for limb migration and 2 for prosthesis stenosis or occlusion). There were 4 cases (10%) of aneurysm enlargement reported at the 4 year follow-up. At 4 years, 38 out of 39 patients were free from type I and III endoleaks. There were no proximal type I or type III endoleaks at 4-year follow-up. Core laboratory evaluation of the postoperative imaging studies indicated absence of endograft migration while a single fracture was demonstrated without any clinical sequelae. The INCRAFT® AAA Stent

  17. Qualidade de fruto da aceroleira cv. Olivier em dois estádios de maturação

    Directory of Open Access Journals (Sweden)

    Elisa Adriano

    2011-10-01

    Full Text Available A acerola é um fruto de grande potencial econômico e nutricional devido ao seu alto teor de vitamina C, destacando-se como alimento funcional. É comercializada principalmente na forma de polpa congelada e fruto in natura. O objetivo deste estudo foi avaliar a qualidade dos frutos da aceroleira cv. Olivier em dois estádios de maturação. Para tanto, foram colhidas amostras de frutos em um pomar comercial no município de Junqueirópolis-SP. Foram realizadas a determinação da cor externa dos frutos e análises das características químicas de teor de sólidos solúveis, pH, acidez titulável, açúcares redutores, 'ratio' e ácido ascórbico de frutos semi-maduros e maduros. Os resultados obtidos permitiram concluir que os frutos semimaduros apresentaram maior acidez total, menor teor de sólidos solúveis e menor concentração de açúcares; no entanto, estes frutos apresentaram maiores teores de vitamina C, expressa em ácido ascórbico. Portanto, quando se buscam altos índices de vitamina C, os frutos devem ser colhidos num estádio de maturação menos avançado, com coloração alaranjada. O estudo demonstrou também que a cv Olivier produz frutos com características adequadas tanto para o mercado in natura quanto para a indústria, apresentando boa coloração e características químicas dentro dos padrões para esta fruta.

  18. 2010 Panel on the Biomaterials Grand Challenges

    Science.gov (United States)

    Reichert, William “Monty”; Ratner, Buddy D.; Anderson, James; Coury, Art; Hoffman, Allan S.; Laurencin, Cato T.; Tirrell, David

    2014-01-01

    In 2009, the National Academy for Engineering issued the Grand Challenges for Engineering in the 21st Century comprised of 14 technical challenges that must be addressed to build a healthy, profitable, sustainable, and secure global community (http://www.engineeringchallenges.org). Although crucial, none of the NEA Grand Challenges adequately addressed the challenges that face the biomaterials community. In response to the NAE Grand Challenges, Monty Reichert of Duke University organized a panel entitled Grand Challenges in Biomaterials at the at the 2010 Society for Biomaterials Annual Meeting in Seattle. Six members of the National Academies—Buddy Ratner, James Anderson, Allan Hoffman, Art Coury, Cato Laurencin, and David Tirrell—were asked to propose a grand challenge to the audience that, if met, would significantly impact the future of biomaterials and medical devices. Successfully meeting these challenges will speed the 60-plus year transition from commodity, off-the-shelf biomaterials to bioengineered chemistries, and biomaterial devices that will significantly advance our ability to address patient needs and also to create new market opportunities. PMID:21171147

  19. Developmental Localization and Methylesterification of Pectin Epitopes during Somatic Embryogenesis of Banana (Musa spp. AAA)

    Science.gov (United States)

    Xu, Chunxiang; Zhao, Lu; Pan, Xiao; Šamaj, Jozef

    2011-01-01

    Background The plant cell walls play an important role in somatic embryogenesis and plant development. Pectins are major chemical components of primary cell walls while homogalacturonan (HG) is the most abundant pectin polysaccharide. Developmental regulation of HG methyl-esterification degree is important for cell adhesion, division and expansion, and in general for proper organ and plant development. Methodology/Principal Findings Developmental localization of pectic homogalacturonan (HG) epitopes and the (1→4)-β-D-galactan epitope of rhamnogalacturonan I (RG-I) and degree of pectin methyl-esterification (DM) were studied during somatic embryogenesis of banana (Musa spp. AAA). Histological analysis documented all major developmental stages including embryogenic cells (ECs), pre-globular, globular, pear-shaped and cotyledonary somatic embryos. Histochemical staining of extracellularly secreted pectins with ruthenium red showed the most intense staining at the surface of pre-globular, globular and pear-shaped somatic embryos. Biochemical analysis revealed developmental regulation of galacturonic acid content and DM in diverse embryogenic stages. Immunodots and immunolabeling on tissue sections revealed developmental regulation of highly methyl-esterified HG epitopes recognized by JIM7 and LM20 antibodies during somatic embryogenesis. Cell walls of pre-globular/globular and late-stage embryos contained both low methyl-esterified HG epitopes as well as partially and highly methyl-esterified ones. Extracellular matrix which covered surface of early developing embryos contained pectin epitopes recognized by 2F4, LM18, JIM5, JIM7 and LM5 antibodies. De-esterification of cell wall pectins by NaOH caused a decrease or an elimination of immunolabeling in the case of highly methyl-esterified HG epitopes. However, immunolabeling of some low methyl-esterified epitopes appeared stronger after this base treatment. Conclusions/Significance These data suggest that both low

  20. Phenylphenalenones Accumulate in Plant Tissues of Two Banana Cultivars in Response to Herbivory by the Banana Weevil and Banana Stem Weevil

    OpenAIRE

    H?lscher, Dirk; Buerkert, Andreas; Schneider, Bernd

    2016-01-01

    Phenylphenalenone-type compounds accumulated in the tissues of two banana cultivars—Musa acuminata cv. “Grande Naine” (AAA) and Musa acuminata × balbisiana Colla cv. “Bluggoe” (ABB)—when these were fed on by the banana weevil (Cosmopolites sordidus (Germ.) (Coleoptera: Curculionidae)) and the banana stem weevil (Odoiporus longicollis (Oliver) (Coleoptera: Curculionidae)). The chemical constituents of the banana material were separated by means of chromatographic techniques and identified by N...

  1. Wilting and biological additive effect on in situ degradability and chemical composition of Arachis pintoi cv Belomonte silage

    OpenAIRE

    Rosana Aparecida Possenti; Evaldo Ferrari Júnior; Valdinei Tadeu Paulino; Ivani Pozar Otsuk; Patrícia Brás

    2010-01-01

    The purpose of this work was to evaluate the effect of wilting and biological additive amendment on chemical composition, fermentation and ruminal degradability of Arachis pintoi cv Belmonte silage. The following treatments were analysed: T1- Arachis pintoi cv Belmonte fresh forage; T2 - Arachis pintoi cv Belmonte fresh forage plus bacterial additive added to the forage prior to the ensilage; T3- Arachis pintoi cv Belmonte wilted by the sun for 4 hours; T4- Arachis pintoi cv Belmonte wilted b...

  2. Analisis Tingkat Kepuasan Kerja Karyawan pada CV Sumber Pangan di Denpasar

    OpenAIRE

    SUPRATMAN, I WAYAN NOVA; USTRIYANA, I NYOMAN GEDE; DEWI, IDA AYU LISTIA

    2016-01-01

    Analysis of Job Satisfaction at CV Food Sources in DenpasarHuman resources is a factor of production which is very important for a company,compared with other factors of production include natural resources, capital, andskills. The purpose of this study was to determine the level of employee satisfactionCV Food Source in Denpasar. The location determination is done deliberately. Thetotal number of the population sampled that stout 40 employees CV Source of Foodin Denpasar. The factors used in...

  3. Preferences of AAA/AAG codon recognition by modified nucleosides, τm5s2U34 and t6A37 present in tRNALys.

    Science.gov (United States)

    Sonawane, Kailas D; Kamble, Asmita S; Fandilolu, Prayagraj M

    2017-12-27

    Deficiency of 5-taurinomethyl-2-thiouridine, τm 5 s 2 U at the 34th 'wobble' position in tRNA Lys causes MERRF (Myoclonic Epilepsy with Ragged Red Fibers), a neuromuscular disease. This modified nucleoside of mt tRNA Lys , recognizes AAA/AAG codons during protein biosynthesis process. Its preference to identify cognate codons has not been studied at the atomic level. Hence, multiple MD simulations of various molecular models of anticodon stem loop (ASL) of mt tRNA Lys in presence and absence of τm 5 s 2 U 34 and N 6 -threonylcarbamoyl adenosine (t 6 A 37 ) along with AAA and AAG codons have been accomplished. Additional four MD simulations of multiple ASL mt tRNA Lys models in the context of ribosomal A-site residues have also been performed to investigate the role of A-site in recognition of AAA/AAG codons. MD simulation results show that, ASL models in presence of τm 5 s 2 U 34 and t 6 A 37 with codons AAA/AAG are more stable than the ASL lacking these modified bases. MD trajectories suggest that τm 5 s 2 U recognizes the codons initially by 'wobble' hydrogen bonding interactions, and then tRNA Lys might leave the explicit codon by a novel 'single' hydrogen bonding interaction in order to run the protein biosynthesis process smoothly. We propose this model as the 'Foot-Step Model' for codon recognition, in which the single hydrogen bond plays a crucial role. MD simulation results suggest that, tRNA Lys with τm 5 s 2 U and t 6 A recognizes AAA codon more preferably than AAG. Thus, these results reveal the consequences of τm 5 s 2 U and t 6 A in recognition of AAA/AAG codons in mitochondrial disease, MERRF.

  4. Pengaruh Budaya Organisasi, Kepemimpinan, dan Motivasi Kerja terhadap Kepuasan Kerja Karyawan CV Surya Raya

    Directory of Open Access Journals (Sweden)

    Laksmi Sito Dwi Irvianti

    2012-05-01

    Full Text Available This research was conducted on CV Surya Raya which is engaged in car shipping. The aim of this study is to determine the influence of organization culture, leadership, and working motivation partially to employees’ satisfaction in CV Surya Raya. The data was collected by conducting interviews with the company and distributing questionnaires to 67 employees as respondents and then analyzed using linear regression analysis method. From the research results, leadership variable does not have a significant impact on employees' job satisfaction in CV Surya Raya. Whereas, the motivation variable has greater influence on job satisfaction of CV Surya Raya employees compared to the organization's culture.

  5. The AAA+ proteins Pontin and Reptin enter adult age: from understanding their basic biology to the identification of selective inhibitors.

    Science.gov (United States)

    Matias, Pedro M; Baek, Sung Hee; Bandeiras, Tiago M; Dutta, Anindya; Houry, Walid A; Llorca, Oscar; Rosenbaum, Jean

    2015-01-01

    Pontin and Reptin are related partner proteins belonging to the AAA+ (ATPases Associated with various cellular Activities) family. They are implicated in multiple and seemingly unrelated processes encompassing the regulation of gene transcription, the remodeling of chromatin, DNA damage sensing and repair, and the assembly of protein and ribonucleoprotein complexes, among others. The 2nd International Workshop on Pontin and Reptin took place at the Instituto de Tecnologia Química e Biológica António Xavier in Oeiras, Portugal on October 10-12, 2014, and reported significant new advances on the mechanisms of action of these two AAA+ ATPases. The major points under discussion were related to the mechanisms through which these proteins regulate gene transcription, their roles as co-chaperones, and their involvement in pathophysiology, especially in cancer and ciliary biology and disease. Finally, they may become anticancer drug targets since small chemical inhibitors were shown to produce anti-tumor effects in animal models.

  6. The AAA+ proteins Pontin and Reptin enter adult age: from understanding their basic biology to the identification of selective inhibitors

    Science.gov (United States)

    Matias, Pedro M.; Baek, Sung Hee; Bandeiras, Tiago M.; Dutta, Anindya; Houry, Walid A.; Llorca, Oscar; Rosenbaum, Jean

    2015-01-01

    Pontin and Reptin are related partner proteins belonging to the AAA+ (ATPases Associated with various cellular Activities) family. They are implicated in multiple and seemingly unrelated processes encompassing the regulation of gene transcription, the remodeling of chromatin, DNA damage sensing and repair, and the assembly of protein and ribonucleoprotein complexes, among others. The 2nd International Workshop on Pontin and Reptin took place at the Instituto de Tecnologia Química e Biológica António Xavier in Oeiras, Portugal on October 10–12, 2014, and reported significant new advances on the mechanisms of action of these two AAA+ ATPases. The major points under discussion were related to the mechanisms through which these proteins regulate gene transcription, their roles as co-chaperones, and their involvement in pathophysiology, especially in cancer and ciliary biology and disease. Finally, they may become anticancer drug targets since small chemical inhibitors were shown to produce anti-tumor effects in animal models. PMID:25988184

  7. The AAA ATPase Vps4 Plays Important Roles in Candida albicans Hyphal Formation and is Inhibited by DBeQ.

    Science.gov (United States)

    Zhang, Yahui; Li, Wanjie; Chu, Mi; Chen, Hengye; Yu, Haoyuan; Fang, Chaoguang; Sun, Ningze; Wang, Qiming; Luo, Tian; Luo, Kaiju; She, Xueping; Zhang, Mengqian; Yang, Dong

    2016-06-01

    Candida albicans is an opportunistic human pathogen, and its pathogenicity is associated with hyphal formation. Previous studies have shown that at neutral-to-alkaline pH, hyphal growth is dependent on the Rim101 pathway whose activation requires Snf7, a member of the ESCRT system. In this work, we described the purification and characterization of the C. albicans Vps4, an AAA ATPase required for recycling of the ESCRTs. Its role on hyphal growth has been investigated. Our data suggest deletion of Vps4 decreases overall hyphal growth at pH 7 and increases the growth of multiple hyphae induced by serum, which indicates that the ESCRTs may make a Rim101-independent contribution to hyphal growth. Furthermore, DBeQ, an inhibitor of the AAA ATPase p97, was shown to inhibit the ATPase activity of Vps4 with an IC50 of about 11.5 μM. To a less degree, it also inhibits hyphal growth. Our work may provide a new strategy to control C. albicans infection.

  8. Cotranslocational processing of the protein substrate calmodulin by an AAA+ unfoldase occurs via unfolding and refolding intermediates.

    Science.gov (United States)

    Augustyniak, Rafal; Kay, Lewis E

    2018-05-22

    Protein remodeling by AAA+ enzymes is central for maintaining proteostasis in a living cell. However, a detailed structural description of how this is accomplished at the level of the substrate molecules that are acted upon is lacking. Here, we combine chemical cross-linking and methyl transverse relaxation-optimized NMR spectroscopy to study, at atomic resolution, the stepwise unfolding and subsequent refolding of the two-domain substrate calmodulin by the VAT AAA+ unfoldase from Thermoplasma acidophilum By engineering intermolecular disulphide bridges between the substrate and VAT we trap the substrate at different stages of translocation, allowing structural studies throughout the translocation process. Our results show that VAT initiates substrate translocation by pulling on intrinsically unstructured N or C termini of substrate molecules without showing specificity for a particular amino acid sequence. Although the B1 domain of protein G is shown to unfold cooperatively, translocation of calmodulin leads to the formation of intermediates, and these differ on an individual domain level in a manner that depends on whether pulling is from the N or C terminus. The approach presented generates an atomic resolution picture of substrate unfolding and subsequent refolding by unfoldases that can be quite different from results obtained via in vitro denaturation experiments.

  9. HTTP as a Data Access Protocol: Trials with XrootD in CMS’s AAA Project

    Science.gov (United States)

    Balcas, J.; Bockelman, B. P.; Kcira, D.; Newman, H.; Vlimant, J.; Hendricks, T. W.; CMS Collaboration

    2017-10-01

    The main goal of the project to demonstrate the ability of using HTTP data federations in a manner analogous to the existing AAA infrastructure of the CMS experiment. An initial testbed at Caltech has been built and changes in the CMS software (CMSSW) are being implemented in order to improve HTTP support. The testbed consists of a set of machines at the Caltech Tier2 that improve the support infrastructure for data federations at CMS. As a first step, we are building systems that produce and ingest network data transfers up to 80 Gbps. In collaboration with AAA, HTTP support is enabled at the US redirector and the Caltech testbed. A plugin for CMSSW is being developed for HTTP access based on the DaviX software. It will replace the present fork/exec or curl for HTTP access. In addition, extensions to the XRootD HTTP implementation are being developed to add functionality to it, such as client-based monitoring identifiers. In the future, patches will be developed to better integrate HTTP-over-XRootD with the Open Science Grid (OSG) distribution. First results of the transfer tests using HTTP are presented in this paper together with details about the initial setup.

  10. An AAA Motor-Driven Mechanical Switch in Rpn11 Controls Deubiquitination at the 26S Proteasome.

    Science.gov (United States)

    Worden, Evan J; Dong, Ken C; Martin, Andreas

    2017-09-07

    Poly-ubiquitin chains direct protein substrates to the 26S proteasome, where they are removed by the deubiquitinase Rpn11 during ATP-dependent substrate degradation. Rapid deubiquitination is required for efficient degradation but must be restricted to committed substrates that are engaged with the ATPase motor to prevent premature ubiquitin chain removal and substrate escape. Here we reveal the ubiquitin-bound structure of Rpn11 from S. cerevisiae and the mechanisms for mechanochemical coupling of substrate degradation and deubiquitination. Ubiquitin binding induces a conformational switch of Rpn11's Insert-1 loop from an inactive closed state to an active β hairpin. This switch is rate-limiting for deubiquitination and strongly accelerated by mechanical substrate translocation into the AAA+ motor. Deubiquitination by Rpn11 and ubiquitin unfolding by the ATPases are in direct competition. The AAA+ motor-driven acceleration of Rpn11 is therefore important to ensure that poly-ubiquitin chains are removed only from committed substrates and fast enough to prevent their co-degradation. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. Triple-A syndrome with prominent ophthalmic features and a novel mutation in the AAAS gene: a case report

    Directory of Open Access Journals (Sweden)

    Stuart Caroline

    2004-06-01

    Full Text Available Abstract Background Triple-A syndrome (Allgrove syndrome is an autosomal recessive disorder characterized by adrenal insufficiency, alacrima, achalasia, and – occasionally – autonomic instability. Mutations have been found in the AAAS gene on 12q13. Case presentation We present the case of a 12 year-old boy with classic systemic features of triple-A syndrome and several prominent ophthalmic features, including: accommodative spasm, dry eye, superficial punctate keratopathy, and pupillary hypersensitivity to dilute pilocarpine. MRI showed small lacrimal glands bilaterally. DNA sequencing of PCR-amplified fragments from the 16 exons of the AAAS gene revealed compound heterozygosity for a new, out-of-frame 5-bp deletion in exon 15, c1368-1372delGCTCA, and a previously-described nonsense mutation in exon 9, c938C>T, R286X. Conclusions In addition to known ophthalmic manifestations, triple-A syndrome can present with accommodative dysregulation and ocular signs of autonomic dysfunction.

  12. Effective Higgs theories in supersymmetric grand unification

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Sibo [Chongqing University, Department of Physics, Chongqing (China)

    2017-09-15

    The effective Higgs theories at the TeV scale in supersymmetric SU(5) grand unification models are systematically derived. Restricted to extensions on 5{sub H} containing the Higgs sector we show that only two types of real (vector-like) models and one type of chiral model are found to be consistent with perturbative grand unification. While the chiral model has been excluded by the LHC data, the fate of perturbative unification will be uniquely determined by the two classes of vector-like models. (orig.)

  13. Grand Tour: immaginario, territorio e culture digitali

    Directory of Open Access Journals (Sweden)

    Emiliano Ilardi

    2016-12-01

    Full Text Available Il Grand Tour può essere recuperato come asset narrativo utile per un intervento strategico di re-branding del viaggio in Italia? Il contributo analizza il contesto e le condizioni per una progettazione di questo livello nell’ambiente culturale dell’epoca digitale. Considerando gli archetipi moderni della mediazione dei luoghi come una grande riserva di senso, da riattivare sia nelle pratiche basate sui format seriali e transmediali che valorizzano i territori nella produzione creativa, sia nella costruzione di infrastrutture digitali e  transluoghi per la valorizzazione degli attrattori culturali.

  14. A Unified Grand Tour of Theoretical Physics

    CERN Document Server

    Lawrie, Ian D

    2002-01-01

    A unified account of the principles of theoretical physics, A Unified Grand Tour of Theoretical Physics, Second Edition stresses the inter-relationships between areas that are usually treated as independent. The profound unifying influence of geometrical ideas, the powerful formal similarities between statistical mechanics and quantum field theory, and the ubiquitous role of symmetries in determining the essential structure of physical theories are emphasized throughout.This second edition conducts a grand tour of the fundamental theories that shape our modern understanding of the physical wor

  15. CIVAC CV-01 type pressure gage for middle vacuum pumps

    International Nuclear Information System (INIS)

    Olaru, Grigore; Aculai, Agustin

    1997-01-01

    The digital display pressure gage CIVAC CV-01 measures absolute pressures in vacuum range of 10 2 - 10 -3 mbar in any installation or equipment generating or using low pressures. It uses a transducer type PIRANI, model TR-02. It is a portable device, easy to exploit and handle. It is applied in research, chemistry, metallurgical industry, mechanical engineering. The system of coupling the transducer to the enclosure where the pressure is to be measured is of type ISO-PNEUROP with flange, collar and adjusting ring with a DN 25 ring gasket. The technical and functional features are: - Measuring error: ± 35% of real conventional value ; - Response time: 20 ms; - Number of measuring points: 1; - Output signal: 0 - 10 V d.c.; - Repeatability error: 5%; - Max weight: 1,600 Kg; Size: 91 x 117 x 187 mm. (authors)

  16. Cytotoxic diterpenoids from Jatropha curcas cv. nigroviensrugosus CY Yang Roots.

    Science.gov (United States)

    Liu, JieQing; Yang, YuanFeng; Xia, JianJun; Li, XuYang; Li, ZhongRong; Zhou, Lin; Qiu, MingHua

    2015-09-01

    An investigation of phytochemicals from the roots of Jatropha curcas cv. nigroviensrugosus resulted in the isolation of twenty diterpenoids, including lathyranlactone, an unusual diterpenoid lactone possessing a 5/13/3 tricyclic skeleton, jatrocurcasenones A-E and jatrophodiones B-E, as well as 10 known analogues. All isolates were evaluated for cytotoxicity against the HL-60, SMMC-772, A-549, MCF-7 and SW480 human tumor cell lines using the MTS viability assay. Four of the known analogues showed cytotoxic activity in these cell lines, with IC50 values ranging from 2.0 to 23.0 μM. Moreover, the assessment of their cytotoxic structure-activity relationships showed the epoxy ring between C-5 and C-6 and the hydroxyl group at C-2 were the key functionalities for cytotoxicity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Sign Language Translator Application Using OpenCV

    Science.gov (United States)

    Triyono, L.; Pratisto, E. H.; Bawono, S. A. T.; Purnomo, F. A.; Yudhanto, Y.; Raharjo, B.

    2018-03-01

    This research focuses on the development of sign language translator application using OpenCV Android based, this application is based on the difference in color. The author also utilizes Support Machine Learning to predict the label. Results of the research showed that the coordinates of the fingertip search methods can be used to recognize a hand gesture to the conditions contained open arms while to figure gesture with the hand clenched using search methods Hu Moments value. Fingertip methods more resilient in gesture recognition with a higher success rate is 95% on the distance variation is 35 cm and 55 cm and variations of light intensity of approximately 90 lux and 100 lux and light green background plain condition compared with the Hu Moments method with the same parameters and the percentage of success of 40%. While the background of outdoor environment applications still can not be used with a success rate of only 6 managed and the rest failed.

  18. Gamma irradiation studies on Gladiolus cv. White Friendship

    International Nuclear Information System (INIS)

    Banerji, B.K.; Datta, S.K.; Sharma, S.C.

    1994-01-01

    Dormant corms of Gladiolus cv. White Friendship were irradiated with 250, 500, 750, 1000 and 1250 Gy of gamma rays. Reduction in survival, plant height, number of leaves and floret, spike length, leaf and corm size and delayed flowering were recorded after irradiation. Morphological abnormalities in foliage and florets and chromosomal aberration during root tip mitosis increased with increase in exposure. Flowering was ceased cent per cent in highest dose of 1250 Gy. LD 50 on survival basis was found in 750 Gy exposure. MV 2 and MV 3 also followed the similar pattern of results as exhibited by MV 1 plants. Pink flower colour mutation was detected in few plants as sectorial chimeric form in MV 2 in treated population. In MV 3 one plant produced spike with lighter pink florets in 750 Gy treatment. This mutant has been isolated in pure form. (author). 10 refs., 2 figs., 2 tabs

  19. Induced somatic mutation in mango, mangifera indica L. cv. Langra

    International Nuclear Information System (INIS)

    Siddiqui, S.H.

    1985-01-01

    Dormant buds of mango (Mangifera indica L. cv. Langra) exposed to acute gamma-irradiation dosages of 1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 kiloroentgens (kR), were grafted on to one-year-old seedling stock. Dosages of 2.0 and 3.0 kR were found satisfactory for the purpose, as measured by bud lethality and scion growth. A bud graft from 3.0 kR bore fruits of excellent quality. Compared with the control, the fruits were heavier, larger and had more creamish-yellow pulp. None of the other morphological changes expressed by the mutant fruits, observed over three fruiting seasons, were disadvantageous. The tree habit is being further investigated before the mutant can be considered for release as an improved cultivar. (author)

  20. Analisis Pengukuran Produktivitas Perusahaan Alsintan CV. Cherry Sarana Agro

    Directory of Open Access Journals (Sweden)

    Prima Fithri

    2016-04-01

    Full Text Available CV Cherry Sarana Agro is a manufacturing company, which produces agricultural machinery. In reality, company evaluatesits work and performance based on level of profits. In order to maintain the company's performance, it is necessary to measures the productivity of company to see how effective the performance of this company. So it can be determined what is needed in order to improve the performance of the company and also can increase profits. Measuring the productivity can be seen from the financial data that obtained from the company. Then based on data processing, show this company's productivity levels decreased during the period of measurement and it means company requires efforts to increase the efficient use of inputs. It is necessary for company to increase its productivity for the future. This improvement was made by taking into account the partial productivity as inputs that affect the profitability of the company on an ongoing basis.

  1. CV activities on the LHC complex during the long shutdown

    CERN Document Server

    Deleval, S; Body, Y; Obrecht, M; Moccia, S; Peon, G

    2011-01-01

    The presentation gives an overview of the major projects and work foreseen to be performed during next long shutdown on cooling and ventilation plants. Several projects are needed following the experience of the last years when LHC was running, in particular the modifications in the water cooling circuits presently in overflow. Some other projects are linked to the CV consolidation plan. Finally, most of the work shall be done to respond to additional requests: SR buildings air conditioning, the need to be able to clean and maintain the LHC cooling towers without a complete stop of cooling circuits, the upgrade of the air conditioning of the CCC rack room cooling etc. For all these activities, the author will detail constraints and the impact on the schedule and on the operation of the plants that will however need to run for most of the shutdown duration. The consequence of postponing the long shutdown from 2012 to 2013 will be also covered.

  2. SU-E-T-516: Dosimetric Validation of AcurosXB Algorithm in Comparison with AAA & CCC Algorithms for VMAT Technique.

    Science.gov (United States)

    Kathirvel, M; Subramanian, V Sai; Arun, G; Thirumalaiswamy, S; Ramalingam, K; Kumar, S Ashok; Jagadeesh, K

    2012-06-01

    To dosimetrically validate AcurosXB algorithm for Volumetric Modulated Arc Therapy (VMAT) in comparison with standard clinical Anisotropic Analytic Algorithm(AAA) and Collapsed Cone Convolution(CCC) dose calculation algorithms. AcurosXB dose calculation algorithm is available with Varian Eclipse treatment planning system (V10). It uses grid-based Boltzmann equation solver to predict dose precisely in lesser time. This study was made to realize algorithms ability to predict dose accurately as its delivery for which five clinical cases each of Brain, Head&Neck, Thoracic, Pelvic and SBRT were taken. Verification plans were created on multicube phantom with iMatrixx-2D detector array and then dose prediction was done with AcurosXB, AAA & CCC (COMPASS System) algorithm and the same were delivered onto CLINAC-iX treatment machine. Delivered dose was captured in iMatrixx plane for all 25 plans. Measured dose was taken as reference to quantify the agreement between AcurosXB calculation algorithm against previously validated AAA and CCC algorithm. Gamma evaluation was performed with clinical criteria distance-to-agreement 3&2mm and dose difference 3&2% in omnipro-I'MRT software. Plans were evaluated in terms of correlation coefficient, quantitative area gamma and average gamma. Study shows good agreement between mean correlation 0.9979±0.0012, 0.9984±0.0009 & 0.9979±0.0011 for AAA, CCC & Acuros respectively. Mean area gamma for criteria 3mm/3% was found to be 98.80±1.04, 98.14±2.31, 98.08±2.01 and 2mm/2% was found to be 93.94±3.83, 87.17±10.54 & 92.36±5.46 for AAA, CCC & Acuros respectively. Mean average gamma for 3mm/3% was 0.26±0.07, 0.42±0.08, 0.28±0.09 and 2mm/2% was found to be 0.39±0.10, 0.64±0.11, 0.42±0.13 for AAA, CCC & Acuros respectively. This study demonstrated that the AcurosXB algorithm had a good agreement with the AAA & CCC in terms of dose prediction. In conclusion AcurosXB algorithm provides a valid, accurate and speedy alternative to AAA

  3. From AAA to Acuros XB-clinical implications of selecting either Acuros XB dose-to-water or dose-to-medium

    International Nuclear Information System (INIS)

    Zifoyda, Jackson M.; Challens, Cameron H.C.; Hsieh, Wen-Long

    2016-01-01

    When implementing Acuros XB (AXB) as a substitute for anisotropic analytic algorithm (AAA) in the Eclipse Treatment Planning System, one is faced with a dilemma of reporting either dose to medium, AXB-Dm or dose to water, AXB-Dw. To assist with decision making on selecting either AXB-Dm or AXB-Dw for dose reporting, a retrospective study of treated patients for head & neck (H&N), prostate, breast and lung is presented. Ten patients, previously treated using AAA plans, were selected for each site and re-planned with AXB-Dm and AXB-Dw. Re-planning was done with fixed monitor units (MU) as well as non-fixed MUs. Dose volume histograms (DVH) of targets and organs at risk (OAR), were analyzed in conjunction with ICRU-83 recommended dose reporting metrics. Additionally, comparisons of plan homogeneity indices (HI) and MUs were done to further highlight the differences between the algorithms. Results showed that, on average AAA overestimated dose to the target volume and OARs by less than 2.0 %. Comparisons between AXB-Dw and AXB-Dm, for all sites, also showed overall dose differences to be small (<1.5 %). However, in non-water biological media, dose differences between AXB-Dw and AXB-Dm, as large as 4.6 % were observed. AXB-Dw also tended to have unexpectedly high 3D maximum dose values (>135 % of prescription dose) for target volumes with high density materials. Homogeneity indices showed that AAA planning and optimization templates would need to be adjusted only for the H&N and Lung sites. MU comparison showed insignificant differences between AXB-Dw relative to AAA and between AXB-Dw relative to AXB-Dm. However AXB-Dm MUs relative to AAA, showed an average difference of about 1.3 % signifying an underdosage by AAA. In conclusion, when dose is reported as AXB-Dw, the effect that high density structures in the PTV has on the dose distribution should be carefully considered. As the results show overall small dose differences between the algorithms, when transitioning

  4. From AAA to Acuros XB-clinical implications of selecting either Acuros XB dose-to-water or dose-to-medium.

    Science.gov (United States)

    Zifodya, Jackson M; Challens, Cameron H C; Hsieh, Wen-Long

    2016-06-01

    When implementing Acuros XB (AXB) as a substitute for anisotropic analytic algorithm (AAA) in the Eclipse Treatment Planning System, one is faced with a dilemma of reporting either dose to medium, AXB-Dm or dose to water, AXB-Dw. To assist with decision making on selecting either AXB-Dm or AXB-Dw for dose reporting, a retrospective study of treated patients for head & neck (H&N), prostate, breast and lung is presented. Ten patients, previously treated using AAA plans, were selected for each site and re-planned with AXB-Dm and AXB-Dw. Re-planning was done with fixed monitor units (MU) as well as non-fixed MUs. Dose volume histograms (DVH) of targets and organs at risk (OAR), were analyzed in conjunction with ICRU-83 recommended dose reporting metrics. Additionally, comparisons of plan homogeneity indices (HI) and MUs were done to further highlight the differences between the algorithms. Results showed that, on average AAA overestimated dose to the target volume and OARs by less than 2.0 %. Comparisons between AXB-Dw and AXB-Dm, for all sites, also showed overall dose differences to be small (135 % of prescription dose) for target volumes with high density materials. Homogeneity indices showed that AAA planning and optimization templates would need to be adjusted only for the H&N and Lung sites. MU comparison showed insignificant differences between AXB-Dw relative to AAA and between AXB-Dw relative to AXB-Dm. However AXB-Dm MUs relative to AAA, showed an average difference of about 1.3 % signifying an underdosage by AAA. In conclusion, when dose is reported as AXB-Dw, the effect that high density structures in the PTV has on the dose distribution should be carefully considered. As the results show overall small dose differences between the algorithms, when transitioning from AAA to AXB, no significant change to existing prescription protocols is expected. As most of the clinical experience is dose-to-water based and calibration protocols and clinical trials are

  5. Propolis extract in postharvest conservation Solo papaya cv. 'Golden'

    Directory of Open Access Journals (Sweden)

    Flávia Regina Passos

    2016-12-01

    Full Text Available The high perishability of papaya (Carica papaya L. reduces its lifespan as well as limits marketing. Coating the fruits is an alternative to aid food preservation. We aimed to evaluate the effects of coating propolis extract on the physicochemical characteristics of papaya stored at room temperature. Solo papayas cv. 'Golden' were randomly divided into five postharvest treatment groups, three forms of dip-coating (70% alcohol, hydroalcoholic extract of propolis to 2.5%, and hydroalcoholic extract of propolis to 5% and two controls (one uncoated and one with refrigerated uncoated fruits. The weight loss, firmness, soluble solids (SS, titratable acidity (TA, SS/TA, and hydrogen potential (pH were evaluated at the intervals of 4 days every 12th storage day. Sensory analysis was performed on the 4th day of storage of papayas and evaluated by untrained through the acceptance testing. Treatments “refrigerated”, “propolis 2.5%”, and “5% propolis” presented with the lowest weight loss. The firmness level for treatment propolis 5% was superior to that of control, alcohol, and 2.5% propolis treatments. The SS was greater for propolis 5% treatment, which only differed from the alcohol treatment. The TA and SS/TA did not vary with the treatments, but with the storage time. The pH of refrigerated papaya showed significant differences in relation to other treatments. The fruits subjected to refrigerated treatment presented with chilling injury. Papayas coated with propolis extract showed sensory acceptability similar to that in other treatments on the 4th day of storage. The coating of propolis extract is a promising alternative for the control of weight loss and for the maintenance of firmness in Solo papaya cv. 'Golden'.

  6. Core Problem: Does the CV Parent Body Magnetization require differentiation?

    Science.gov (United States)

    O'Brien, T.; Tarduno, J. A.; Smirnov, A. V.

    2016-12-01

    Evidence for the presence of past dynamos from magnetic studies of meteorites can provide key information on the nature and evolution of parent bodies. However, the suggestion of a past core dynamo for the CV parent body based on the study of the Allende meteorite has led to a paradox: a core dynamo requires differentiation, evidence for which is missing in the meteorite record. The key parameter used to distinguish core dynamo versus external field mechanisms is absolute field paleointensity, with high values (>>1 μT) favoring the former. Here we explore the fundamental requirements for absolute field intensity measurement in the Allende meteorite: single domain grains that are non-interacting. Magnetic hysteresis and directional data define strong magnetic interactions, negating a standard interpretation of paleointensity measurements in terms of absolute paleofield values. The Allende low field magnetic susceptibility is dominated by magnetite and FeNi grains, whereas the magnetic remanence is carried by an iron sulfide whose remanence-carrying capacity increases with laboratory cycling at constant field values, indicating reordering. The iron sulfide and FeNi grains are in close proximity, providing mineralogical context for interactions. We interpret the magnetization of Allende to record the intense early solar wind with metal-sulfide interactions amplifying the field, giving the false impression of a higher field value in some prior studies. An undifferentiated CV parent body is thus compatible with Allende's magnetization. Early solar wind magnetization should be the null hypothesis for evaluating the source of magnetization for chondrites and other meteorites.

  7. Hilbert's Grand Hotel with a series twist

    Science.gov (United States)

    Wijeratne, Chanakya; Mamolo, Ami; Zazkis, Rina

    2014-08-01

    This paper presents a new twist on a familiar paradox, linking seemingly disparate ideas under one roof. Hilbert's Grand Hotel, a paradox which addresses infinite set comparisons is adapted and extended to incorporate ideas from calculus - namely infinite series. We present and resolve several variations, and invite the reader to explore his or her own variations.

  8. "Teine" võitis Prantsusmaal Grand Prix'

    Index Scriptorium Estoniae

    2006-01-01

    Prantsusmaal Essonne'is toimuval 8. Euroopa filmifestivalil Cinessonne sai üliõpilaste žürii grand prix rahvusvahelises ühistöös valminud tantsufilm "Teine" ("Another") : režissöör Rene Vilbre. Ka teistest festivalidest, kus film osalenud

  9. The Virtual Grand Tour as Educational Paradigm

    DEFF Research Database (Denmark)

    Hansen, Per Skafte; Mouritsen, Lars

    2001-01-01

    The Virtual Grand Tour as defined here bears some resemblance to its 18th century ancestor: a wide range of individual topics are treated as a whole; a tutor, whether real or simulated, present or remote, is provided; a set of problem solving tools forms an integrated part of the "traveller...

  10. Grand Prix Eurovision: Eine Fankultur im Medienzeitalter

    Directory of Open Access Journals (Sweden)

    Heinz Moser

    2017-06-01

    Full Text Available Der Grand Prix Eurovision ist seit Jahrzehnten eine der bekanntesten Unterhaltungssendungen im europäischen Raum. Dennoch erregte es Verwunderung, wenn der Schreibende Bekannten darüber berichtete, daß dies ein Forschungsgegenstand sei. Wurde von Interviews mit Grand Prix-Fans erzählt, so fielen schnell Aussagen wie: „Wie kann man sich nur für so etwas Abseitiges und Triviales wie den Grand Prix interessieren“. Dennoch bin ich der Meinung, daß Fankulturen für die entstehende Mediengesellschaft ein nicht unwichtiges Forschungsthema darstellen. Zwar geht es nicht um eine medienpädagogische Fragestellung im engeren Sinne; die Fans des Grand Prix Eurovision sind dem Jugendalter längst entwachsen. Dennoch handelt es sich bei Fangemeinschaften um Phänomene, die im Rahmen von Jugend- und Kinderkulturen von besonderer Relevanz sind. So meint Winter (1997, daß jugendliche Fanwelten eine bedeutende Rolle als Kristallisationspunkte kultureller Differenzierung spielen: ,Die Zugehörigkeit zu einer Fan weit ist Teil der jugendlichen Lebensbewältigung in der Postmoderne, denn in der Gemeinschaft der Fans können Jugendliche emotionale Allianzen eingehen, außeralltäglichen Beschäftigungen nachgehen, expressive Identitätsmuster gemeinschaftlich realisieren und sich mit ihrer Lebenssituation als Heranwachsende auseinandersetzen“ (Winter 1997, S. 51f..

  11. Grand Canyon Monitoring and Research Center

    Science.gov (United States)

    Hamill, John F.

    2009-01-01

    The Grand Canyon of the Colorado River, one of the world's most spectacular gorges, is a premier U.S. National Park and a World Heritage Site. The canyon supports a diverse array of distinctive plants and animals and contains cultural resources significant to the region's Native Americans. About 15 miles upstream of Grand Canyon National Park sits Glen Canyon Dam, completed in 1963, which created Lake Powell. The dam provides hydroelectric power for 200 wholesale customers in six western States, but it has also altered the Colorado River's flow, temperature, and sediment-carrying capacity. Over time this has resulted in beach erosion, invasion and expansion of nonnative species, and losses of native fish. Public concern about the effects of Glen Canyon Dam operations prompted the passage of the Grand Canyon Protection Act of 1992, which directs the Secretary of the Interior to operate the dam 'to protect, mitigate adverse impacts to, and improve values for which Grand Canyon National Park and Glen Canyon National Recreation Area were established...' This legislation also required the creation of a long-term monitoring and research program to provide information that could inform decisions related to dam operations and protection of downstream resources.

  12. Comprehensive District Reform: Philadelphia's Grand Experiment.

    Science.gov (United States)

    Useem, Elizabeth; Balfanz, Robert

    2002-01-01

    This report describes "Philadelphia's Grand Experiment" in comprehensive school district reform, from its conception through its initial months of implementation. In 2001, as part of the remedy for low student performance, the governor ordered the state to take over governance of the Philadelphia School District, with a substantial…

  13. Rio Branco, grand strategy and naval power

    Directory of Open Access Journals (Sweden)

    João Paulo Alsina Jr.

    2014-12-01

    Full Text Available This article addresses Baron of Rio Branco's grand strategy and the role played by the naval reorganization program (1904-1910 in this context. The ensuing case study determined the domestic and international constraints that affected the program, as well as the worldview of the patron of Brazilian diplomacy regarding military power's instrumentality to foreign policy.

  14. Reisipakkumine - Grand Tour Itaalias / Mai Levin

    Index Scriptorium Estoniae

    Levin, Mai, 1942-

    2009-01-01

    Tiina Abeli koostatud ja Urmas Viigi kujundatud näitus "Grand Tour. Eesti kunstnikud Itaalias" Kumu Kunstimuuseumis 05. aprillini. Loetletud eksponeeritud tööde autoreid. Näitus annab ülevaate, kes siinsetest kunstnikest 19. sajandi algusest kuni 1930ndate aastateni Itaalias käisid ja kuidas see nende loomingut mõjutas

  15. Middle Rio Grande Basin Research Report 2008

    Science.gov (United States)

    Deborah M. Finch; Catherine Dold

    2008-01-01

    An ecosystem is rarely static. A natural system composed of plants, animals, and microorganisms interacting with an area's physical factors, an ecosystem is always fluctuating and evolving. But sometimes, often at the hands of humans, ecosystems change too much. Such is the case with many of the ecosystems of the Middle Rio Grande Basin of New Mexico.

  16. Panel - Rio Grande restoration: Future directions

    Science.gov (United States)

    Deborah M. Finch; Pete V. Domenici; Jeffrey. C. Whitney; Steve Harris; Brian Shields; Clifford S. Crawford

    1996-01-01

    The purpose of this panel was to discuss historical and current changes to the Rio Grande system, focusing on the middle Basin, and to present and review different individual, organizational, and political perspectives on the future of the system. Invitations were made to panelists based on their past and current interests and activities pertaining to restoration of...

  17. Death of pastures syndrome: tissue changes in Urochloa hybrida cv. Mulato II and Urochloa brizantha cv. Marandu

    Directory of Open Access Journals (Sweden)

    N. G. Ribeiro-Júnior

    Full Text Available Abstract The quality of forage production is a prerequisite to raising livestock. Therefore, income losses in this activity, primarily cattle raising, can result in the impossibility of economic activity. Through the qualitative and quantitative anatomical study of Urochloa hybrida cv. Mulato II and U. brizantha cv. Marandu, we searched for descriptions and compared changes in the individual vegetative body from populations with death syndrome pastures (DPS. Specimens were collected at different physiological stages from farms in northern Mato Grosso. After collection, the individuals were fixed in FAA50 and stored in 70% alcohol. Histological slides were prepared from the middle third of the sections of roots, rhizomes, and leaves, and the proportions and characteristics of tissues were evaluated in healthy, intermediate, and advanced stages of DPS. Changes were compared between cultivars. With the advancement of the syndrome, the following changes were observed: a more marked decrease in the length of roots in U. hybrida; disorganization of the cortical region of the roots and rhizome cultivars; fungal hyphae in roots and aerenchyma formation in U. hybrida; a decrease in sclerenchyma fiber proportions in roots and leaves; sclerification of the epidermis of U. brizantha rhizomes; and an increase in pericyclic fibers in U. hybrida. Furthermore, there was a decrease in the volume of epidermal cells of the abaxial face of the leaves of both cultivars, with a greater reduction in U. hybrida; a gradual decrease in thickness in the midrib of leaves similar to leaf mesophyll; conduction system obstructions; partial or total cell lysis in roots and rhizomes affected by the syndrome. Obstructions in sieve tube element and companion cells, and sometimes obstruction in xylem vessel elements. The evolution of DPS in cultivars was similar, but there were variations, arising probably from the physiological response to stress, such as aerenchyma formation in

  18. How Adequate is your CV? Analyzing French CVs with ReaderBench

    NARCIS (Netherlands)

    Gutu, Gabriel; Dascalu, Mihai; Trausan-Matu, Stefan; Lepoivre, Olivier

    2017-01-01

    This study is aimed at presenting a new ReaderBench-based tool built to support candidates in increasing the quality of their CV for a job opening. Both the visual quality and the textual content are considered while also providing an overview and corresponding feedback for the entire CV.

  19. CV-Online tahab Londoni börsile / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Personaliotsinguga tegelev firma tahab tulevikus minna Londoni ja OMXi börsile. Diagramm: CV-Online Group loodab tänavu esmakordselt positiivse kulumieelse ärikasumi. Vt. samas: Üks CV-Online'i aktsionäridest on ka Google'i aktsionär; Väsinud ootamast esimest IPOt

  20. PERUMUSAN STRATEGI BERSAING JAHE INSTAN PRODUK CV. INTRAFOOD SURAKARTA MENGGUNAKAN PERCEPTUAL MAPPING

    Directory of Open Access Journals (Sweden)

    Mohd. Harisudin

    2013-11-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui posisi bersaing jahe instan produk CV. Intrafood dan strategi bersaing yang dapat direkomendasikan untuk meraih keberasilannya. Metode dasar yang digunakan adalah analisis deskriptif. Penentuan lokasi penelitian ditentukan dengan metode purposive, yaitu CV. Intrafood Surakarta. Jenis data yang digunakan dalam penelitian ini adalah data primer dan data sekunder. Metode analisis data menggunakan analisis Perceptual Mapping. Dari hasil penelitian diperoleh informasi bahwa jahe instan produk CV Intrafood berada pada peringkat ke-2 dari produk empat jahe instan yang diperbandingkan. Atribut yang dapat dijadikan kekuatan utama dalam meningkatkan pemasaran jahe instan produk CV. Intrafood adalah manfaat produk. Sisi yang paling lemah adalah atribut desain kemasan dan kinerja produk dalam kemasan. Abstract This study aims to determine the competitive position of the product instant ginger CV. Intrafood and competitive strategy can be recommended to achieve success. The basic method used is descriptive analysis. Determining the location of the study are determined by purposive method, namely CV. Intrafood Surakarta. Data used in this study is primary data and secondary data. Method analyzed using Perceptual Mapping. From the results of the study concluded that instant ginger of CV Intrafood product ranks second of four instant ginger products are compared. Attributes that can be used as a major force in improving product marketing instant ginger CV. Intrafood is the benefits of the product. The weakest side is the packaging design and performance attributes of the product in the packaging.

  1. Postural stabilizing effect of alfacalcidol and active absorbable algal calcium (AAA Ca) compared with calcium carbonate assessed by computerized posturography.

    Science.gov (United States)

    Fujita, Takuo; Nakamura, Shoji; Ohue, Mutsumi; Fujii, Yoshio; Miyauchi, Akimitsu; Takagi, Yasuyuki; Tsugeno, Hirofumi

    2007-01-01

    Sway and postural instability have drawn attention as a risk factor for osteoporotic fracture, in addition to low bone mineral density (BMD) and poor bone quality. In view of the fracture-reducing effect of alfacalcidol and active absorbable algal calcium (AAA Ca) not readily explained by rather mild increases of BMD, attempts were made to evaluate postural stabilizing effect of alfacalcidol, AAA Ca, and calcium carbonate (CaCO(3)) by computerized posturography. Track of the gravity center was analyzed to calculate parameters related to tract length, track range, and track density to express the degree of sway before and after supplementation in 126 subjects ranging in age between 20 and 81 years randomly divided into four groups. Supplementation with AAA Ca containing 900 mg elemental Ca (group A), no calcium (group B), CaCO(3) also containing 900 mg elemental Ca (group C), or alfacalcidol (group D) continued daily for 12 months. For each parameter, the ratio closed eye value/open eye value (Romberg ratio) was calculated to detect aggravation of sway by eye closure. Age, parameters of Ca and P, and proportions of subjects with fracture and those with low BMD showed no marked deviation among the groups. With eyes open, significant decreases of a track range parameter (REC) from group B was noted in groups A (P = 0.0397) and D (P = 0.0296), but not in group C according to multiple comparison by Scheffe, indicating superior postural stabilizing effect of A and D over C. In the first 2 months, a significant fall was already evident in REC from group B in group D (P = 0.0120) with eyes open. Paired comparison of sway parameters before and after supplementation revealed a significant increase of track density parameter (LNGA), indicating sway control efficiency and a significant decrease of REC in groups A and D compared to group B with eyes open. With eyes closed, only group A showed a significant improvement from group B (P = 0.0456; Fig. 1), with a significant

  2. ANALISIS KELAYAKAN INVESTASI PENAMBAHAN MESIN FRAIS BARU PADA CV. XYZ

    Directory of Open Access Journals (Sweden)

    Febri Muhammad Rachadian

    2013-04-01

    Full Text Available Perkembangan teknologi sangat pesat sehingga dunia perindustrian harus mengikuti perkembangannya. Penerapan teknologi diharapkan dapat meningkatkan proses produksi sehingga dapat menghasilkan produk yang berkualitas. Pada industri pembuatan mesin cetak di CV XYZ, mesin-mesin yang digunakan telah hampir lebih dari 20 tahun. Ada indikasi bahwa mesin-mesin tersebut sudah lewat umur pakainya. Alternatif yang digunakan selama ini ialah penggantian komponen yang rusak. Peneliti ingin membandingkan alternative tersebut dengan  penambahan mesin frais baru. Untuk itu diperlukan analisis kelayakan investasi penambahan mesin baru. Dalam perkiraan kurun waktu 10 tahun dan suku bunganya 15 % dengan menggunakan NPV, penambahan mesin frais menarik sebesar Rp 461.201.000 dibandingkan penggantian komponen yang rusak sebesar Rp 211.227.000. Sebaliknya pada PBP, penggantian komponen yang rusak masih mengungguli dengan 1 tahun 4 bulan dibandingkan penambahan mesin frais baru selama 2 tahun 1 bulan. Begitupun juga PI penggantian komponen yang rusak masih menarik sebesar 4,2 dibandingkan PI penambahan mesin frais baru sebesar 2,56. Dapat disimpulkan bahwa dalam jangka waktu 10 tahun, alternatif penggantian komponen yang rusak masih lebih baik dibandingkan penambahan mesin frais baru. Namun, untuk investasi jangka panjang, mungkin saja alternatif penambahan mesin frais baru lebih menarik. Kata Kunci: penggantian komponen rusak, mesin frais baru, mesin cetak Abstract The rapid development of technology makes the world of industry must follow its development. Application of technology is expected to improve the production process so it can be produce a quality product. On the industrial manufacture of printing machines in CV XYZ, the machines have been used almost more than 20 years. There are indications that those machines passed the age lifetime. The alternatives that have been used during this time are the replacement of defective parts. Researchers wanted to

  3. A novel two-step mechanism for removal of a mitochondrial signal sequence involves the mAAA complex and the putative rhomboid protease Pcp1.

    Science.gov (United States)

    Esser, Karlheinz; Tursun, Baris; Ingenhoven, Martin; Michaelis, Georg; Pratje, Elke

    2002-11-08

    The yeast protein cytochrome c peroxidase (Ccp1) is nuclearly encoded and imported into the mitochondrial intermembrane space, where it is involved in degradation of reactive oxygen species. It is known, that Ccp1 is synthesised as a precursor with a N-terminal pre-sequence, that is proteolytically removed during transport of the protein. Here we present evidence for a new processing pathway, involving novel signal peptidase activities. The mAAA protease subunits Yta10 (Afg3) and Yta12 (Rca1) were identified both to be essential for the first processing step. In addition, the Pcp1 (Ygr101w) gene product was found to be required for the second processing step, yielding the mature Ccp1 protein. The newly identified Pcp1 protein belongs to the rhomboid-GlpG superfamily of putative intramembrane peptidases. Inactivation of the protease motifs in mAAA and Pcp1 blocks the respective steps of proteolysis. A model of coupled Ccp1 transport and N-terminal processing by the mAAA complex and Pcp1 is discussed. Similar processing mechanisms may exist, because the mAAA subunits and the newly identified Pcp1 protein belong to ubiquitous protein families.

  4. Comparison of Acuros (AXB) and Anisotropic Analytical Algorithm (AAA) for dose calculation in treatment of oesophageal cancer: effects on modelling tumour control probability

    International Nuclear Information System (INIS)

    Padmanaban, Sriram; Warren, Samantha; Walsh, Anthony; Partridge, Mike; Hawkins, Maria A

    2014-01-01

    To investigate systematic changes in dose arising when treatment plans optimised using the Anisotropic Analytical Algorithm (AAA) are recalculated using Acuros XB (AXB) in patients treated with definitive chemoradiotherapy (dCRT) for locally advanced oesophageal cancers. We have compared treatment plans created using AAA with those recalculated using AXB. Although the Anisotropic Analytical Algorithm (AAA) is currently more widely used in clinical routine, Acuros XB (AXB) has been shown to more accurately calculate the dose distribution, particularly in heterogeneous regions. Studies to predict clinical outcome should be based on modelling the dose delivered to the patient as accurately as possible. CT datasets from ten patients were selected for this retrospective study. VMAT (Volumetric modulated arc therapy) plans with 2 arcs, collimator rotation ± 5-10° and dose prescription 50 Gy / 25 fractions were created using Varian Eclipse (v10.0). The initial dose calculation was performed with AAA, and AXB plans were created by re-calculating the dose distribution using the same number of monitor units (MU) and multileaf collimator (MLC) files as the original plan. The difference in calculated dose to organs at risk (OAR) was compared using dose-volume histogram (DVH) statistics and p values were calculated using the Wilcoxon signed rank test. The potential clinical effect of dosimetric differences in the gross tumour volume (GTV) was evaluated using three different TCP models from the literature. PTV Median dose was apparently 0.9 Gy lower (range: 0.5 Gy - 1.3 Gy; p < 0.05) for VMAT AAA plans re-calculated with AXB and GTV mean dose was reduced by on average 1.0 Gy (0.3 Gy −1.5 Gy; p < 0.05). An apparent difference in TCP of between 1.2% and 3.1% was found depending on the choice of TCP model. OAR mean dose was lower in the AXB recalculated plan than the AAA plan (on average, dose reduction: lung 1.7%, heart 2.4%). Similar trends were seen for CRT plans

  5. Comparison of Acuros (AXB) and Anisotropic Analytical Algorithm (AAA) for dose calculation in treatment of oesophageal cancer: effects on modelling tumour control probability.

    Science.gov (United States)

    Padmanaban, Sriram; Warren, Samantha; Walsh, Anthony; Partridge, Mike; Hawkins, Maria A

    2014-12-23

    To investigate systematic changes in dose arising when treatment plans optimised using the Anisotropic Analytical Algorithm (AAA) are recalculated using Acuros XB (AXB) in patients treated with definitive chemoradiotherapy (dCRT) for locally advanced oesophageal cancers. We have compared treatment plans created using AAA with those recalculated using AXB. Although the Anisotropic Analytical Algorithm (AAA) is currently more widely used in clinical routine, Acuros XB (AXB) has been shown to more accurately calculate the dose distribution, particularly in heterogeneous regions. Studies to predict clinical outcome should be based on modelling the dose delivered to the patient as accurately as possible. CT datasets from ten patients were selected for this retrospective study. VMAT (Volumetric modulated arc therapy) plans with 2 arcs, collimator rotation ± 5-10° and dose prescription 50 Gy / 25 fractions were created using Varian Eclipse (v10.0). The initial dose calculation was performed with AAA, and AXB plans were created by re-calculating the dose distribution using the same number of monitor units (MU) and multileaf collimator (MLC) files as the original plan. The difference in calculated dose to organs at risk (OAR) was compared using dose-volume histogram (DVH) statistics and p values were calculated using the Wilcoxon signed rank test. The potential clinical effect of dosimetric differences in the gross tumour volume (GTV) was evaluated using three different TCP models from the literature. PTV Median dose was apparently 0.9 Gy lower (range: 0.5 Gy - 1.3 Gy; p AAA plans re-calculated with AXB and GTV mean dose was reduced by on average 1.0 Gy (0.3 Gy -1.5 Gy; p AAA plan (on average, dose reduction: lung 1.7%, heart 2.4%). Similar trends were seen for CRT plans. Differences in dose distribution are observed with VMAT and CRT plans recalculated with AXB particularly within soft tissue at the tumour/lung interface, where AXB has been shown to more

  6. Interaction studies of resistomycin from Streptomyces aurantiacus AAA5 with calf thymus DNA and bovine serum albumin

    Science.gov (United States)

    Vijayabharathi, R.; Sathyadevi, P.; Krishnamoorthy, P.; Senthilraja, D.; Brunthadevi, P.; Sathyabama, S.; Priyadarisini, V. Brindha

    2012-04-01

    Resistomycin, a secondary metabolite produced by Streptomyces aurantiacus AAA5. The binding interaction of resistomycin with calf thymus DNA (CT DNA) and bovine serum albumin (BSA) was investigated by spectrophotometry, spectrofluorimetry, circular dichroism (CD) and synchronous fluorescence techniques under physiological conditions in vitro. Absorption spectral studies along with the fluorescence competition with ethidium bromide measurements and circular dichroism clearly suggest that the resistomycin bind with CT DNA relatively strong via groove binding. BSA interaction results revealed that the drug was found to quench the fluorescence intensity of the protein through a static quenching mechanism. The number of binding sites 'n' and apparent binding constant 'K' calculated according to the Scatchard equation exhibit a good binding property to bovine serum albumin protein. In addition, the results observed from synchronous fluorescence measurements clearly demonstrate the occurrence of conformational changes of BSA upon addition of the test compound.

  7. Nitrogen fertilization on the establishment of Arachis pintoi cv. Belmonte

    Directory of Open Access Journals (Sweden)

    Rita Manuele Porto Sales

    2012-11-01

    Full Text Available The objective was to evaluate the effect of nitrogen fertilization on the establishment of forage peanut (Arachis pintoi cv. Belmonte propagated vegetatively. The experiment was conducted in a greenhouse in a completely randomized design with treatments arranged in a 2 × 4 factorial design - two ages (70 and 85 days after planting and four nitrogen doses (0, 40, 80 and 120 kg/ha - with four replications. Morphogenetic and structural characteristics and production were evaluated. The nitrogen accelerated the establishment of the forage peanut with an increase in dry weight of green leaves and stolons. The greatest length of stolons (48.0 cm was obtained with a dose equivalent to 86 kg N/ha and higher density of stolons (20 stolons/vase between 78 and 82 kg N/ha. Nitrogen fertilization also reduced the phyllochron from 6.7 to 4.6 days/leaf. These data were more intense at 85 days, suggesting greater photosynthetic contribution during this period related to the large number of leaves after 70 days. Therefore, nitrogen can be an important tool to accelerate the establishment of pure stands of forage peanut.

  8. EVALUASI SISTEM INFORMASI PERSEDIAAN PADA CV. SARANA TELEMAXINDO

    Directory of Open Access Journals (Sweden)

    Nelly Nelly

    2010-10-01

    Full Text Available Evaluation objectives of inventory information systems at CV. Facilities Telemaxindo was to evaluate the weaknesses of the implementation of internal control, as well as estimating and assessing possible risks that might arise. The research method used is literature study of reference books in the library, and field study consisted of observation, interviews with relevant parties, creating check lists, check the inventory information systems documentation, and testing of inventory application systems. The results are findings of control weaknesses in the implementation of security management, operational management control, boundary control, input control, and output control on the present inventory information system. These weaknesses could pose a risk and if not dealt soon it could hurt the company. The resulting conclusion of the security management control, boundary control, control input, and output control is not running well so that it needs to be improved in accordance with the recommendations, while operational management control has been running fairly well.Keywords: evaluation, information system, inventory

  9. Infrared small target detection technology based on OpenCV

    Science.gov (United States)

    Liu, Lei; Huang, Zhijian

    2013-09-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  10. Seven health physics calculator programs for the HP-41CV

    International Nuclear Information System (INIS)

    Rittmann, P.D.

    1984-08-01

    Several user-oriented programs for the Hewlett-Packard HP-41CV are explained. The first program builds, stores, alters, and ages a list of radionuclides. This program only handles single- and double-decay chains. The second program performs convenient conversions for the six nuclides of concern in plutonium handling. The conversions are between mass, activity, and weight percents of the isotopes. The source can be aged and/or neutron generation rates can be computed. The third program is a timekeeping program that improves the process of manually estimating and tracking personnel exposure during high dose rate tasks by replacing the pencil, paper, and stopwatch method. This program requires a time module. The remaining four programs deal with computations of time-integrated air concentrations at various distances from an airborne release. Building wake effects, source depletion by ground deposition, and sector averaging can all be included in the final printout of the X/Q - Hanford and X/Q - Pasquill programs. The shorter versions of these, H/Q and P/Q, compute centerline or sector-averaged values and include a subroutine to facilitate dose estimation by entering dose factors and quantities released. The horizontal and vertical dispersion parameters in the Pasquill-Gifford programs were modeled with simple, two-parameter functions that agreed very well with the usual textbook graphs. 8 references, 7 appendices

  11. A virtual computing infrastructure for TS-CV SCADA systems

    CERN Document Server

    Poulsen, S

    2008-01-01

    In modern data centres, it is an emerging trend to operate and manage computers as software components or logical resources and not as physical machines. This technique is known as â€ワvirtualisation” and the new computers are referred to as â€ワvirtual machines” (VMs). Multiple VMs can be consolidated on a single hardware platform and managed in ways that are not possible with physical machines. However, this is not yet widely practiced for control system deployment. In TS-CV, a collection of VMs or a â€ワvirtual infrastructure” is installed since 2005 for SCADA systems, PLC program development, and alarm transmission. This makes it possible to consolidate distributed, heterogeneous operating systems and applications on a limited number of standardised high-performance servers in the Central Control Room (CCR). More generally, virtualisation assists in offering continuous computing services for controls and maintaining performance and assuring quality. Implementing our systems in a vi...

  12. MICROPROPAGAÇÃO DO KIWI CV. HAYWARD

    Directory of Open Access Journals (Sweden)

    SCALIZE FÁBIO EDUARDO

    2001-01-01

    Full Text Available O presente trabalho teve como objetivo desenvolver um protocolo para a obtenção de mudas de kiwi (Actinidia chinensis Planch, cv. Hayward, por meio do cultivo in vitro de cotilédones. Utilizou-se o meio de MURASHIGE & SKOOG (1962 -- MS, suplementado com dois tipos de auxina (AIA e AIB e uma citocinina (BAP. Foram verificados os efeitos de três doses de auxinas (0,125; 0,250 e 0,375 mg.L-1, combinadas com três doses de citocinina (0,5; 1,0 e 1,5 mg.L-1 na capacidade morfogênica dos explantes. Procedeu-se o estudo histológico dos órgãos das plântulas obtidas in vitro, e verificou-se, também, a capacidade de aclimatação das mudas ex vitro. A menor dose de AIB (0,125 mg.L-1, independentemente das doses de BAP, foi a mais eficaz na morfogênese dos explantes. Não foram verificadas alterações histológicas e anatômicas das plântulas obtidas in vitro. Aos três meses após o cultivo ex vitro dos explantes, verificou-se a sobrevivência de 88% das plantas transplantadas em condições de campo.

  13. Antidiabetic and anticancer activities of Mangifera indica cv. Okrong leaves

    Science.gov (United States)

    Ganogpichayagrai, Aunyachulee; Palanuvej, Chanida; Ruangrungsi, Nijsiri

    2017-01-01

    Diabetes and cancer are a major global public health problem. Plant-derived agents with undesirable side-effects were required. This study aimed to evaluate antidiabetic and anticancer activities of the ethanolic leaf extract of Mangifera indica cv. Okrong and its active phytochemical compound, mangiferin. Antidiabetic activities against yeast α-glucosidase and rat intestinal α-glucosidase were determined using 1 mM of p-nitro phenyl-α-D-glucopyranoside as substrate. Inhibitory activity against porcine pancreatic α-amylase was performed using 1 mM of 2-chloro-4 nitrophenol-α-D-maltotroside-3 as substrate. Nitrophenol product was spectrophotometrically measured at 405 nm. Anticancer activity was evaluated against five human cancer cell lines compared to two human normal cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Mango leaf extract and mangiferin exhibited dose-dependent inhibition against yeast α-glucosidase with the IC50 of 0.0503 and 0.5813 mg/ml, respectively, against rat α-glucosidase with the IC50 of 1.4528 and 0.4333 mg/ml, respectively, compared to acarbose with the IC50 of 11.9285 and 0.4493 mg/ml, respectively. For anticancer activity, mango leaf extract, at ≥200 μg/ml showed cytotoxic potential against all tested cancer cell lines. In conclusion, mango leaf possessed antidiabetic and anticancer potential in vitro. PMID:28217550

  14. AAA-ATPase NVL2 acts on MTR4-exosome complex to dissociate the nucleolar protein WDR74

    Energy Technology Data Exchange (ETDEWEB)

    Hiraishi, Nobuhiro; Ishida, Yo-ichi; Nagahama, Masami, E-mail: nagahama@my-pharm.ac.jp

    2015-11-20

    Nuclear VCP-like 2 (NVL2) is a chaperone-like nucleolar ATPase of the AAA (ATPase associated with diverse cellular activities) family, which exhibits a high level of amino acid sequence similarity with the cytosolic AAA-ATPase VCP/p97. These proteins generally act on macromolecular complexes to stimulate energy-dependent release of their constituents. We previously showed that NVL2 interacts with RNA processing/degradation machinery containing an RNA helicase MTR4/DOB1 and an exonuclease complex, nuclear exosome, and involved in the biogenesis of 60S ribosomal subunits. These observations implicate NVL2 as a remodeling factor for the MTR4-exosome complex during the maturation of pre-ribosomal particles. Here, we used a proteomic screen and identified a WD repeat-containing protein 74 (WDR74) as a factor that specifically dissociates from this complex depending on the ATPase activity of NVL2. WDR74 shows weak amino acid sequence similarity with the yeast ribosome biogenesis protein Nsa1 and is co-localized with NVL2 in the nucleolus. Knockdown of WDR74 decreases 60S ribosome levels. Taken together, our results suggest that WDR74 is a novel regulatory protein of the MTR4-exsosome complex whose interaction is regulated by NVL2 and is involved in ribosome biogenesis. - Highlights: • WDR74 accumulates in MTR4-exosome complex upon expression of dominant-negative NVL2. • WDR74 is co-localized with NVL2 in the nucleolus. • WDR74, along with NVL2, is involved in the synthesis of 60S ribosomal subunits.

  15. AAA-ATPase NVL2 acts on MTR4-exosome complex to dissociate the nucleolar protein WDR74

    International Nuclear Information System (INIS)

    Hiraishi, Nobuhiro; Ishida, Yo-ichi; Nagahama, Masami

    2015-01-01

    Nuclear VCP-like 2 (NVL2) is a chaperone-like nucleolar ATPase of the AAA (ATPase associated with diverse cellular activities) family, which exhibits a high level of amino acid sequence similarity with the cytosolic AAA-ATPase VCP/p97. These proteins generally act on macromolecular complexes to stimulate energy-dependent release of their constituents. We previously showed that NVL2 interacts with RNA processing/degradation machinery containing an RNA helicase MTR4/DOB1 and an exonuclease complex, nuclear exosome, and involved in the biogenesis of 60S ribosomal subunits. These observations implicate NVL2 as a remodeling factor for the MTR4-exosome complex during the maturation of pre-ribosomal particles. Here, we used a proteomic screen and identified a WD repeat-containing protein 74 (WDR74) as a factor that specifically dissociates from this complex depending on the ATPase activity of NVL2. WDR74 shows weak amino acid sequence similarity with the yeast ribosome biogenesis protein Nsa1 and is co-localized with NVL2 in the nucleolus. Knockdown of WDR74 decreases 60S ribosome levels. Taken together, our results suggest that WDR74 is a novel regulatory protein of the MTR4-exsosome complex whose interaction is regulated by NVL2 and is involved in ribosome biogenesis. - Highlights: • WDR74 accumulates in MTR4-exosome complex upon expression of dominant-negative NVL2. • WDR74 is co-localized with NVL2 in the nucleolus. • WDR74, along with NVL2, is involved in the synthesis of 60S ribosomal subunits.

  16. Cosmological implications of grand unified theories

    International Nuclear Information System (INIS)

    Nanopoulos, D.V.

    1982-01-01

    These lectures, mainly devoted to the cosmological implications of GUTs, also include the essential ingredients of GUTs and some of their important applications to particle physics. Section 1 contains some basic points concerning the structure of the standard strong and electroweak interactions prior to grand unification. A detailed expose of GUTs is attempted in sect. 2, including their basci principles and their consequences for particle physics. The minimal, simplest GUT, SU 5 is analysed in some detail and it will be used throughout these lectures as the GUT prototype. Finally, sect. 3 contains the most important cosmological implications of GUTs, including baryon number generation in the early Universe (in rather lengthy detail), dissipative processes in the very early Universe, grand unified monopoles, etc. (orig./HSI)

  17. The geology of Piz Pian Grand

    International Nuclear Information System (INIS)

    Huber, M.; Staeuble, J.

    1987-01-01

    Nagra has identified four potential sites for a repository for low- and intermediate-level waste. Exploration work is already underway at Oberbauenstock (UR) and Piz Pian Grand (GR). As part of the investigations in the Piz Pian Grand area, geological surface mapping was carried out between 1984 and 1987. Since the data obtained is still being evaluated, it would be premature to draw any interpretative conclusions at this stage. On the other hand, some of the most significant observations of this work can be summarised here. As a first step, the geological framework in which these investigations are to be seen should be defined. Observations will then be made on the rock content (lithology) and geometric structure (structural geology) of the area. (author) 6 figs

  18. Grand Gulf-prioritization of regulatory requirements

    International Nuclear Information System (INIS)

    Meisner, M.J.

    1993-01-01

    As cost pressures mount, Grand Gulf nuclear station (GGNS) is relying increasingly on various prioritization approaches to implement, modify, eliminate, or defer regulatory requirements. Regulatory requirements can be prioritized through the use of three measures: (1) safety (or risk) significance; (2) cost; and (3) public policy (or political) significance. This paper summarizes GGNS' efforts to implement solutions to regulatory issues using these three prioritization schemes to preserve a balance between cost and safety benefit

  19. Between two evils: Investors prefer grand corruption!

    OpenAIRE

    Graf Lambsdorff, Johann

    2005-01-01

    Recent empirical studies claim that, in addition to levels of corruption, investors are deterred by its unpredictability. I claim instead that it is petty corruption that deters investors. I employ seven subcomponents of corruption for a sample of 102 countries that appear in the 2003 Global Competitiveness Report of the WEF. The second principal component of this data depicts a grand, political type, embracing corruption in government policymaking and in judicial decisions as opposed to corr...

  20. Grand Challenges in Music Information Research

    OpenAIRE

    Goto, Masataka

    2012-01-01

    This paper discusses some grand challenges in which music information research will impact our daily lives and our society in the future. Here, some fundamental questions are how to provide the best music for each person, how to predict music trends, how to enrich human-music relationships, how to evolve new music, and how to address environmental, energy issues by using music technologies. Our goal is to increase both attractiveness and social impacts of music information research in the fut...

  1. Meliponiculture in Rio Grande do Norte

    Directory of Open Access Journals (Sweden)

    Ulysses Madureira Maia

    2015-12-01

    Full Text Available ABSTRACT. Maia U.M., Jaffe R., Carvalho A.T. & Imperatriz-Fonseca V.L. [Meliponiculture in Rio Grande do Norte.] Meliponicultura no Rio Grande do Norte. Revista Brasileira de Medicina Veterinária, 37(4:327-333, 2015. Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes, 3900, Monte Alegre, Ribeirão Preto, SP 14040-901, Brasil. E-mail: ummaia@usp.br This study aimed to assess the current status of stingless bee beekeeping (meliponiculture in the State of Rio Grande do Norte, Brazil, with the aid of structured questionnaires made during visits to beekeepers. The results were compared with a previous census made in the state and with a similar study from Australia. Meliponiculture in Rio Grande do Norte is still informal and little standardized. The activity has grown in recent years considering the mean number of nests per beekeeper. Most apiaries are formed of up to 50 colonies, usually distributed in the backyards of homes. Twelve species of stingless bees were reared in the state, and the most common was the “Jandaíra” bee (Melipona subnitida, whose honey is considered medicinal. While many beekeepers already know the importance of bees as pollinators, stingless bees are still not used for crop pollination. Compared to a recent analysis of beekeeping in Australia, meliponiculture in Brazil is more traditional, honey is the main product and the number of colonies per beekeeper is much higher. Our results highlight the need to reinforce knowledge about bees and promote specific training aimed at improving and standardizing management practices.

  2. Neutrino mixing in a grand unified theory

    International Nuclear Information System (INIS)

    Milton, K.; Tanaka, K.

    1980-01-01

    Neutrino mixing in a grand unified theory in which the neutrino mass matrix is determined by the Gell-Mann-Ramond-Slansky mechanism was investigated. With an arbitrary real right-handed Majorana mass matrix which incorporates three neutrino mass scales, the effects of the up-quark mass matrix are found to be dominant and as a result no significant mixing of ν/sub e/ occurs, while ν/sub μ/ - ν/sub γ/ mixing can be substantial

  3. The Grand Strategy of Charles de Gaulle

    Science.gov (United States)

    1989-09-08

    4. TITLE AND SUBTITLE The Grand Startegy of Charles de Gaulle 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S...dependent on French influence within NATO and the Common Market . De Gaulle frequently used these fora to veto British and American initiatives. As Cook...the reserve currency. Gold would be the basis of international finance and a French-dominated European Common Market would provide the framework

  4. Comportamento das cultivares Cabernet Sauvignon e Syrah em diferentes porta-enxertos Yield-related performance of cv Cabernet Sauvingnon and Petite Syrah on different rootstocks

    Directory of Open Access Journals (Sweden)

    Tânia das Graças Silva Orlando

    2008-06-01

    Full Text Available As cultivares Cabernet Sauvignon e Syrah são destinadas à elaboração de vinhos tintos jovens ou de guarda bastante difundidas em diversas zonas vitivinícolas de todo o mundo. No Brasil, são utilizadas principalmente no Rio Grande do Sul e Vale do Rio São Francisco. Atualmente, produtores da região de Jundiaí, SP, têm demonstrado interesse na utilização de tais cultivares com intuito de obter vinhos de qualidade superior aos originários de cultivares americanas. Por isso, realizou-se o estudo do comportamento das cultivares Cabernet Sauvignon e Syrah em diferentes porta-enxertos, na região de Jundiaí, SP (23º06' S; 46º55' W, 715 m. Não houve influência dos diferentes porta-enxertos sobre o comportamento da cultivar Cabernet Sauvignon. O porta-enxerto IAC 571-6 'Jundiaí' tendeu a conferir colheita tardia na cultivar Syrah. Os porta-enxertos IAC 572 'Jales' e IAC 571-6 'Jundiaí' conferiram maior produção a cultivar Syrah. A cultivar Syrah apresentou-se como boa opção de plantio para obtenção de vinho de qualidade na região.Grapevines cv. Cabernet Sauvignon and Syrah are largely used for high quality vine making all around the world. In Brazil they are concentrated moreover, at the Rio Grande do Sul state and in the São Francisco Valley. Producers from Jundiaí region (São Paulo state have been interested in such cultivars for improved quality vine making as they generally use american cultivars for that propose. Aiming study the performance of cv Cabernet Sauvingnon and Syrah, cultivated over different rootstocks an experiment was conducted in the region of Jundiaí (SP (23º06' S; 46º55' W, 715 m. Results showed that there wasn't influence of different rootstocks on the performance of both analyzed cultivars. It was observed a trend of the rootstock IAC 571-6 'Jundiaí' to improve final yield of cv Syrah. The Rootstocks IAC 572 'Jales' and IAC 571-6 'Jundiaí' combined to Syrah resulted in the highest yield in the

  5. [Grand Banks activity : updates and opportunities

    International Nuclear Information System (INIS)

    Bruce, G.

    1998-01-01

    An overview of the exploration and on-going activities by the petroleum industry on the Grand Banks of Newfoundland was presented. The two offshore oil developments underway are Hibernia and Terra Nova, both located in the Jeanne d'Arc Basin. Current production from Hibernia is 68,000 bopd, expected to rise to 130,000 bopd in 1999. The Terra Nova Field is still under development. Total recoverable reserves from the 17 discoveries made in the Jeanne d'Arc Basin are estimated at 1.6 billion barrels of oil and 4 trillion cubic feet of gas. Industry participants in the area include Amoco, Petro-Canada, Mobil, Chevron, Husky and Norsk Hydro. Petro-Canada believes the Grand Banks represent one of the best opportunities for oil anywhere in the world. There are currently 21 exploration licenses held on the Grand Banks. Major attractions of the area include the large reserve potential, the relatively low finding costs, the size of the pools being discovered, improvements in offshore technology that have substantially lowered development costs, and a profit-sensitive generic royalty regime that ensures reasonable rates of return for investors. figs

  6. Proton decay in grand unified theories

    International Nuclear Information System (INIS)

    Lucha, W.

    1984-01-01

    Interactions which violate the conservation of baryon and lepton number represent an intrinsic part of all grand unified theories (GUTs) of strong and electroweak interactions. These new interactions - predicted within the framework of GUTs - generate B and L violating four-fermion interactions via the exchange of superheavy particles which cannot be ascribed a well-defined baryon or lepton number. The effective coupling constant of these four-fermion interactions might be large enough to make the proton decay detectable by the present generation of experiments. In this review the basic concepts of conventional as well as supersymmetric GUTs relevant for proton decay are sketched. The baryon number violating sector of grand unified theories is discussed in more detail. Special emphasis is laid on the various selection rules arising as consequences of low-energy gauge invariance and supersymmetry for proton decay. These selection rules already determine the coarse pattern of the resulting decay modes and branching ratios without any reference to or detailed knowledge of the underlying grand unified theory. Finally the numerous theoretical predictions are summarized and confronted with experiment. (Author)

  7. Raptor Use of the Rio Grande Gorge

    Energy Technology Data Exchange (ETDEWEB)

    Ponton, David A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-03-20

    The Rio Grande Gorge is a 115 km long river canyon located in Southern Colorado (15 km) and Northern New Mexico (100 km). The majority of the canyon is under the administration of the Bureau of Land Management {BLM), and 77 km of the canyon south of the Colorado/New Mexico border are designated Wild River under the National Wild and Scenic Rivers Act of 1968. Visits I have made to the Rio Grande Gorge over the past 15 .years disclosed some raptor utilization. As the Snake River Birds of Prey Natural Area gained publicity, its similarity to the Rio Grande Gorge became obvious, and I was intrigued by the possibility of a high raptor nesting density in the Gorge. A survey in 1979 of 20 km of the northern end of the canyon revealed a moderately high density of red-tailed hawks and prairie falcons. With the encouragement of that partial survey, and a need to assess the impact of river-running on nesting birds of prey, I made a more comprehensive survey in 1980. The results of my surveys, along with those of a 1978 helicopter survey by the BLM, are presented in this report, as well as general characterization of the area, winter use by raptors, and an assessment of factors influencing the raptor population.

  8. Assembly of the Lactuca sativa, L. cv. Tizian draft genome sequence reveals differences within major resistance complex 1 as compared to the cv. Salinas reference genome.

    Science.gov (United States)

    Verwaaijen, Bart; Wibberg, Daniel; Nelkner, Johanna; Gordin, Miriam; Rupp, Oliver; Winkler, Anika; Bremges, Andreas; Blom, Jochen; Grosch, Rita; Pühler, Alfred; Schlüter, Andreas

    2018-02-10

    Lettuce (Lactuca sativa, L.) is an important annual plant of the family Asteraceae (Compositae). The commercial lettuce cultivar Tizian has been used in various scientific studies investigating the interaction of the plant with phytopathogens or biological control agents. Here, we present the de novo draft genome sequencing and gene prediction for this specific cultivar derived from transcriptome sequence data. The assembled scaffolds amount to a size of 2.22 Gb. Based on RNAseq data, 31,112 transcript isoforms were identified. Functional predictions for these transcripts were determined within the GenDBE annotation platform. Comparison with the cv. Salinas reference genome revealed a high degree of sequence similarity on genome and transcriptome levels, with an average amino acid identity of 99%. Furthermore, it was observed that two large regions are either missing or are highly divergent within the cv. Tizian genome compared to cv. Salinas. One of these regions covers the major resistance complex 1 region of cv. Salinas. The cv. Tizian draft genome sequence provides a valuable resource for future functional and transcriptome analyses focused on this lettuce cultivar. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. ANALISIS PENGUKURAN PRODUKTIVITAS PADA CV. CITRA JEPARA FURNITURE

    Directory of Open Access Journals (Sweden)

    Darminto Pujotomo

    2012-02-01

    Full Text Available CV. Citra Jepara adalah perusahaan furnitur ekspor. Pada masa itu, perusahaan hanya melihat level keuntungan untuk mengkaji perusahaan telah berjalan lebih baik atau kebalikannya. Hasil persepsi membuat perusahaan hanya sedikit memperhatikan efisiensi pemakaian sumber daya yang dimiliki (input sehingga perusahaan membutuhkan biaya besar untuk melakukan aktivitas perusahaan.Penelitian ini mempelajari evaluasi produktivitas perusahaan dan pengukuran serta hubungan antara level produktivitas dengan level profitabilitas (kemampuan perusahaan untuk menciptakan laba yang dapat dicapai oleh perusahaan.Hasil penelitian ini tentang pengukuran produktivitas parsial memperlihatkan level produktivitas berubah-ubah dan masih memerlukan usaha peningkatan produktivitas parsial terutama dalam pemakaian masing-masing input dengan efisien. Level Total Factor Productivity selama perioda pengukuran memperlihatkan level pertumbuhan positif. Ini melukiskan perusahaan sukses dalam mengelola input tenaga kerja dan modal kolektif yang dimiliki. Level Total Productivity selama perioda pengukuran menunjukkan level pertumbuhan yang berubah-ubah. Ini melukiskan perusahaan itu belum dapat mengelola input yang dimiliki keseluruhan dengan sukses. Untuk melukiskan hubungan antara produktivitas total dengan profitabilitas dari hasil penelitian bisa diketahui faktor produktivitas (0,998 mempunyai pengaruh lebih besar dibandingkan pemulihan harga (0,457 untuk peningkatan profitabilitas perusahaan selama kurun waktu satu tahun perioda pengukuran. Diperlukan usaha untuk meningkatkan profitabilitas perusahaan untuk periode mendatang disertai usaha untuk meningkatkan produktivitas yang memusatkan pada peningkatan produktivitas material dan produktivitas modal sebagai input yang sangat mempengaruhi profitabilitas perusahaan dengan cara berkelanjutkan dan berkesinambungan. Kata kunci: pengukuran evaluasi dan produktivitas, profitabilitas    CV. Citra Jepara is an export furniture

  10. Novel circulatory connection from the acupoint Zhong Wan(CV12 to pancreas

    Directory of Open Access Journals (Sweden)

    Minsoo Kim

    2008-03-01

    Full Text Available Objectives : Demonstrating a novel circulatory path from the acupoint(CV12 to the pancreas. Method : Alcian blue(1% solution, 20μl, pH 7.4 was injected into the acupoint(CV12. Two hours later the surfaces of internal organs were observed by using a stereomicroscope. Results : Alcian blue arrived and colored the omental fat band(OFB on the pancreas. The OFB connected the head and tail of the pancreas, the pancreas and the spleen, and the pancreas and the stomach. Conclusion : The existence of a novel circulatory path from the acupoint CV12 to the pancreas and its OFB was demonstrated.

  11. Analisis Strategi Mitigasi Resiko Pada Supply Chain CV Surya Cip Dengan House of Risk Model

    OpenAIRE

    Pertiwi, Yoana Ellen; Susanty, Aries

    2017-01-01

    Analysis of Risk Mitigation Strategies on CV Surya CIP's Supply Chain Using House of Risk Model. In the supply chain activities always has the potential of risk, therefore risk management is necessary for handling the risks. At the company that produces plastic molding machine such as CV Surya CIP, in its supply chain activities have a chance to arise risks. The purpose of this study is to identify the various risks that occur in the CV Surya CIP and its causes, severity and determine the ...

  12. Analisis Pengawasan Logistik Produk Aqua Ukuran 330ml Pada CV. Dlu'x Resto Samarinda

    OpenAIRE

    Mardiana, Ali Masuhud, H. Mulyadi Syp

    2016-01-01

    The problem in this research is "Are Determination Against Aqua Products Logistics Control 330ml sizes on CV. DLux Resto has been optimized? "This study aims to determine the amount of inventory on the CV aqua 330ml sizes. Dlu'x Resto in Samarinda.Formulation of the problem in this study is whether the determination of the logistical monitoring product inventory aqua 330ml sizes that have been carried out on the CV. Dlu'x Resto Samarinda already performed optimally.The hypothesis in this stud...

  13. Fitomassa e produção de algodoeiro cv. BRS Jady cultivado com águas salinas e doses de esterco bovino

    Directory of Open Access Journals (Sweden)

    Leandro de Pádua Souza

    2016-11-01

    Full Text Available A produção de algodoeiro de fibra naturalmente colorido, na região semiárida do nordeste brasileiro, onde as águas nem sempre são de boa qualidade, está na dependência do uso de técnicas que viabilizem o manejo do solo e da água com teor elevado de sais. Diante do exposto, objetivou-se avaliar fitomassa e produção do algodoeiro cv. BRS Jady submetido a níveis crescentes de salinidade da água de irrigação e doses de esterco bovino. O experimento foi conduzido em ambiente protegido em um Neossolo Regolítico Eutrófico de textura franco-arenosa no município de Campina Grande, Paraíba. Usou-se o delineamento experimental de blocos ao acaso, em esquema fatorial 4 x 4, com três repetições, sendo os tratamentos compostos de quatro níveis de condutividade elétrica da água (CEa (1,7; 3,4; 5,1 e 6,8 dS m-1 e quatro doses de esterco bovino (0; 2,5; 3,5 e 4,5% em base do volume do solo. Níveis crescentes de salinidade da água de irrigação com CEa superior a 1,7 dS m-1 reduziu a formação de fitomassa seca de folha, entretanto o aumento nas doses de esterco bovino promoveu acréscimos nesta variável. A adubação com esterco bovino promove incremento na produção de número de sementes totais e massa de cem sementes. Houve interação entre os fatores níveis de condutividade elétrica da água de irrigação e doses de esterco bovino para fitomassa seca e caule e raiz do algodoeiro cv. ‘BRS Jady’.Fitomassa and cotton production cv. BRS Jady cultivated with salt waters and esterco bovine dosesAbstract: The production of naturally colored fiber cotton in the semi-arid region of northeastern Brazil, where water is not always of good quality, is dependent on the use of techniques that make it possible to manage soil and water with high salt content. In view of the above, the objective was to evaluate phytomass and cotton production cv. BRS Jady subjected to increasing levels of salinity of irrigation water and doses of bovine

  14. SU-F-T-600: Influence of Acuros XB and AAA Dose Calculation Algorithms On Plan Quality Metrics and Normal Lung Doses in Lung SBRT

    International Nuclear Information System (INIS)

    Yaparpalvi, R; Mynampati, D; Kuo, H; Garg, M; Tome, W; Kalnicki, S

    2016-01-01

    Purpose: To study the influence of superposition-beam model (AAA) and determinant-photon transport-solver (Acuros XB) dose calculation algorithms on the treatment plan quality metrics and on normal lung dose in Lung SBRT. Methods: Treatment plans of 10 Lung SBRT patients were randomly selected. Patients were prescribed to a total dose of 50-54Gy in 3–5 fractions (10?5 or 18?3). Doses were optimized accomplished with 6-MV using 2-arcs (VMAT). Doses were calculated using AAA algorithm with heterogeneity correction. For each plan, plan quality metrics in the categories- coverage, homogeneity, conformity and gradient were quantified. Repeat dosimetry for these AAA treatment plans was performed using AXB algorithm with heterogeneity correction for same beam and MU parameters. Plan quality metrics were again evaluated and compared with AAA plan metrics. For normal lung dose, V_2_0 and V_5 to (Total lung- GTV) were evaluated. Results: The results are summarized in Supplemental Table 1. PTV volume was mean 11.4 (±3.3) cm"3. Comparing RTOG 0813 protocol criteria for conformality, AXB plans yielded on average, similar PITV ratio (individual PITV ratio differences varied from −9 to +15%), reduced target coverage (−1.6%) and increased R50% (+2.6%). Comparing normal lung doses, the lung V_2_0 (+3.1%) and V_5 (+1.5%) were slightly higher for AXB plans compared to AAA plans. High-dose spillage ((V105%PD - PTV)/ PTV) was slightly lower for AXB plans but the % low dose spillage (D2cm) was similar between the two calculation algorithms. Conclusion: AAA algorithm overestimates lung target dose. Routinely adapting to AXB for dose calculations in Lung SBRT planning may improve dose calculation accuracy, as AXB based calculations have been shown to be closer to Monte Carlo based dose predictions in accuracy and with relatively faster computational time. For clinical practice, revisiting dose-fractionation in Lung SBRT to correct for dose overestimates attributable to algorithm

  15. SU-F-T-600: Influence of Acuros XB and AAA Dose Calculation Algorithms On Plan Quality Metrics and Normal Lung Doses in Lung SBRT

    Energy Technology Data Exchange (ETDEWEB)

    Yaparpalvi, R; Mynampati, D; Kuo, H; Garg, M; Tome, W; Kalnicki, S [Montefiore Medical Center, Bronx, NY (United States)

    2016-06-15

    Purpose: To study the influence of superposition-beam model (AAA) and determinant-photon transport-solver (Acuros XB) dose calculation algorithms on the treatment plan quality metrics and on normal lung dose in Lung SBRT. Methods: Treatment plans of 10 Lung SBRT patients were randomly selected. Patients were prescribed to a total dose of 50-54Gy in 3–5 fractions (10?5 or 18?3). Doses were optimized accomplished with 6-MV using 2-arcs (VMAT). Doses were calculated using AAA algorithm with heterogeneity correction. For each plan, plan quality metrics in the categories- coverage, homogeneity, conformity and gradient were quantified. Repeat dosimetry for these AAA treatment plans was performed using AXB algorithm with heterogeneity correction for same beam and MU parameters. Plan quality metrics were again evaluated and compared with AAA plan metrics. For normal lung dose, V{sub 20} and V{sub 5} to (Total lung- GTV) were evaluated. Results: The results are summarized in Supplemental Table 1. PTV volume was mean 11.4 (±3.3) cm{sup 3}. Comparing RTOG 0813 protocol criteria for conformality, AXB plans yielded on average, similar PITV ratio (individual PITV ratio differences varied from −9 to +15%), reduced target coverage (−1.6%) and increased R50% (+2.6%). Comparing normal lung doses, the lung V{sub 20} (+3.1%) and V{sub 5} (+1.5%) were slightly higher for AXB plans compared to AAA plans. High-dose spillage ((V105%PD - PTV)/ PTV) was slightly lower for AXB plans but the % low dose spillage (D2cm) was similar between the two calculation algorithms. Conclusion: AAA algorithm overestimates lung target dose. Routinely adapting to AXB for dose calculations in Lung SBRT planning may improve dose calculation accuracy, as AXB based calculations have been shown to be closer to Monte Carlo based dose predictions in accuracy and with relatively faster computational time. For clinical practice, revisiting dose-fractionation in Lung SBRT to correct for dose overestimates

  16. Circulating Vascular Basement Membrane Fragments are Associated with the Diameter of the Abdominal Aorta and Their Expression Pattern is Altered in AAA Tissue.

    Science.gov (United States)

    Holsti, Mari; Wanhainen, Anders; Lundin, Christina; Björck, Martin; Tegler, Gustaf; Svensson, Johan; Sund, Malin

    2018-04-12

    Abdominal aortic aneurysm (AAA) is characterised by enhanced proteolytic activity, and extracellular matrix (ECM) remodelling in the vascular wall. Type IV and XVIII collagen/endostatin are structural proteins in vascular basement membrane (VBM), a specialised ECM structure. Here the association between plasma levels of these collagens with the aortic diameter and expansion rate is studied, and their expression in aortic tissue characterised. This was a retrospective population based cohort study. Type IV and XVIII collagen/endostatin were analysed in plasma by ELISA assay in 615 men, divided into three groups based on the aortic diameter: 1) normal aorta ≤ 25 mm, 2) sub-aneurysmal aorta (SAA) 26-29 mm, and 3) AAA ≥ 30 mm. Follow up data were available for 159 men. The association between collagen levels and aortic diameter at baseline, and with the expansion rate at follow up were analysed in ordinal logistic regression and linear regression models, controlling for common confounding factors. Tissue expression of the collagens was analysed in normal aorta (n = 6) and AAA (n = 6) by immunofluorescence. Plasma levels of type XVIII collagen/endostatin (136 ng/mL [SD 29] in individuals with a normal aorta diameter, 154 ng/ml [SD 45] in SAA, and 162 ng/ml [SD 46] in AAA; p = .001) and type IV collagen (105 ng/mL [SD 42] normal aorta, 124 ng/ml [SD 46] SAA, and 127 ng/ml [SD 47] AAA; p = .037) were associated with a larger aortic diameter. A significant association was found between the baseline levels of type XVIII/endostatin and the aortic expansion rate (p = .035), but in the multivariable model, only the initial aortic diameter remained significantly associated with expansion (p = .005). Altered expression patterns of both collagens were observed in AAA tissue. Plasma levels of circulating type IV and XVIII collagen/endostatin increase with AAA diameter. The expression pattern of VBM proteins is altered in the aneurysm wall. Copyright

  17. Fox grape cv. Bordô (Vitis labrusca L.) and grapevine cv. Chardonnay (Vitis vinifera L.) cultivated in vitro under different carbohydrates, amino acids and 6-Benzylaminopurine levels

    OpenAIRE

    Carvalho, Dayse Cristina de; Silva, André Luís Lopes da; Schuck, Mariane Ruzza; Purcino, Marivel; Tanno, Guilherme Nakao; Biasi, Luiz Antonio

    2013-01-01

    The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine) levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1 were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine wa...

  18. Limited geographic distribution of the novel cyclovirus CyCV-VN.

    Science.gov (United States)

    Le, Van Tan; de Jong, Menno D; Nguyen, Van Kinh; Nguyen, Vu Trung; Taylor, Walter; Wertheim, Heiman F L; van der Ende, Arie; van der Hoek, Lia; Canuti, Marta; Crusat, Martin; Sona, Soeng; Nguyen, Hanh Uyen; Giri, Abhishek; Nguyen, Thi Thuy Chinh Bkrong; Ho, Dang Trung Nghia; Farrar, Jeremy; Bryant, Juliet E; Tran, Tinh Hien; Nguyen, Van Vinh Chau; van Doorn, H Rogier

    2014-02-05

    A novel cyclovirus, CyCV-VN, was recently identified in cerebrospinal fluid (CSF) from patients with central nervous system (CNS) infections in central and southern Vietnam. To explore the geographic distribution of this novel virus, more than 600 CSF specimens from patients with suspected CNS infections in northern Vietnam, Cambodia, Nepal and The Netherlands were screened for the presence of CyCV-VN but all were negative. Sequence comparison and phylogenetic analysis between CyCV-VN and another novel cyclovirus recently identified in CSF from Malawian patients indicated that these represent distinct cycloviral species, albeit phylogenetically closely related. The data suggest that CyCV-VN has a limited geographic distribution within southern and central Vietnam. Further research is needed to determine the global distribution and diversity of cycloviruses and importantly their possible association with human disease.

  19. Analisis Pengaruh Lingkungan Kerja dan Pemberian Kompensasi terhadap Kinerja Karyawan CV Mum Indonesia

    Directory of Open Access Journals (Sweden)

    Jerry M. Logahan

    2012-05-01

    Full Text Available Mum CV is a company engaged in the sale of bread. To increase sales of bread (performance it needs to investigate the influence of work environment and compensation of employees. The method of analysis used in this study is descriptive, Pearson Regression, and Multiple Regression. The data were obtained from the employees performance by completing the questionnaires provided using Likert scale which was useful to determine the level of disagreement questions on the questionnaires. Results achieved in this study are the work environment has no significant influence on employees performance in CV Mum Indonesia, amounting to 0,068. Compensation has significant, no influence on employees performance in CV Mum Indonesia that is equal to 0,580 and there is a significant effect of 33.6%. Work environment and compensation have a significant impact on employees performance in CV Mum Indonesia. It is equal to 0,580 and there is a significant effect of 33.6%.

  20. LEP constraints on grand unified theories

    International Nuclear Information System (INIS)

    Sarkar, Utpal

    1993-01-01

    Recent developments on grand unified theories (GUTs) in the context of the LEP measurements of the coupling constants are reviewed. The three coupling constants at the electroweak scale have been measured at LEP quite precisely. One can allow these couplings to evolve with energy following the renormalization group equations for the various groups and find out whether all the coupling constants meet at any energy. It was pointed out that the minimal SU(5) grand unified theory fails to satisfy this test. However, various extensions of the theory are still allowed. These extensions include (i) supersymmetric SU(5) GUT, with some arbitrariness in the susy breaking scale arising from the threshold corrections, (ii) non-susy SU(5) GUTs with additional fermions as well as Higgs multiplets, which has masses of the order of TeV, and (iii) non-renormalizable effect of gravity with a fine tuned relation among the coupling constants at the unification energy. The LEP results also constrain GUTs with an intermediate symmetry breaking scale. By adjusting the intermediate symmetry breaking scale, one usually can have unification, but these theories get constrained. For example, the left-right symmetric theories coming from GUTs can be broken only at energies higher than about ∼10 10 GeV. This implies that if right handed gauge bosons are found at energies lower than this scale, then that will rule out the possibility of grand unification. Another recent interesting development on the subject, namely, low energy unification, is discussed in this context. All the coupling constants are unified at energies of the order of ∼10 8 GeV when they are embedded in an SU(15)GUT, with some particular symmetry breaking pattern. But even in this case the results of the intermediate symmetry breaking scale remain unchanged. (author). 16 refs., 3 figs

  1. Maternal and fetal outcome in grand multipara

    International Nuclear Information System (INIS)

    Qamar, A.; Qamar, S.

    2015-01-01

    Study Design: Case control study. Place and Duration of Study: Gynecology and Obstetric Unit-I of the Jinnah Post Graduate and Medical Centre Karachi, from February 2009 to January 2010. Patients and Methods: One hundred (100) patients of grand multipara (GMP), (parity = 5) and 100 patients of multipara (MP) (parity 2-4) were included in the study. Pregnant women with known medical conditions including essential hypertension, diabetes mellitus, epilepsy, primigravidas, women with previous caesarean section and twin pregnancies were excluded. Patients were admitted through antenatal clinic and emergency. A detailed history was taken and a physical examination was done with special emphasis on obstetrical examination. Investigations like blood CP, Urine D/R, blood grouping and sonogram were done. During labour, mother and neonates were managed according to ward protocols. Maternal and fetal outcomes were compared among GMPs and MPs. Results: A high frequency of anaemia (81% vs 20%), pregnancy induced hypertension (45%, vs. 26%) and gestational diabetes (9%, vs1%) were seen in GMP as compared to MP group. Frequency of malpresentations (26% vs 15%), postpartum hemorrhage (15%, vs 10%) and intrauterine deaths (26%, vs 13%) were higher in GMP group along with a high caesarean delivery rate (GMP 21%, MP 14%). A higher maternal mortality (GMP 4%, MP 1%) and low APGAR score (GMP 12%, MP 4%) were observed among babies born to grand multipara group. Conclusion: Grand multiparity is associated with adverse outcome for both mother and fetus. Effort should be directed to reduce high parity in the community through effective family planning initiatives. Specialized antenatal and obstetrical care facilities should be available. (author)

  2. Biofuels from the Fresh Water Microalgae Chlorella vulgaris (FWM-CV for Diesel Engines

    Directory of Open Access Journals (Sweden)

    Saddam H. Al-lwayzy

    2014-03-01

    Full Text Available This work aims to investigate biofuels for diesel engines produced on a lab-scale from the fresh water microalgae Chlorella vulgaris (FWM-CV. The impact of growing conditions on the properties of biodiesel produced from FWM-CV was evaluated. The properties of FWM-CV biodiesel were found to be within the ASTM standards for biodiesel. Due to the limited amount of biodiesel produced on the lab-scale, the biomass of dry cells of FWM-CV was used to yield emulsified water fuel. The preparation of emulsion fuel with and without FWM-CV cells was conducted using ultrasound to overcome the problems of large size microalgae colonies and to form homogenized emulsions. The emulsified water fuels, prepared using ultrasound, were found to be stable and the size of FWM-CV colonies were effectively reduced to pass through the engine nozzle safely. Engine tests at 3670 rpm were conducted using three fuels: cottonseed biodiesel CS-B100, emulsified cottonseed biodiesel water fuel, water and emulsifier (CS-E20 and emulsified water containing FWM-CV cells CS-ME20. The results showed that the brake specific fuel consumption (BSFC was increased by about 41% when the engine was fueled with emulsified water fuels compared to CS-B100. The engine power, exhaust gas temperature, NOx and CO2 were significantly lower than that produced by CS-B100. The CS-ME20 produced higher power than CS-E20 due to the heating value improvement as a result of adding FWM-CV cells to the fuel.

  3. Analisis Pengaruh Kemampuan, Usaha dan Dukungan Perusahaan terhadap Kinerja Karyawan pada CV Sandang Gloria Konveksindo

    OpenAIRE

    Logahan, Jerry; Sari, Synthia Atas; Marisa, Dian

    2012-01-01

    Clothing Konveksindo CV Gloria is a trading company engaged in the sale of products and clothing items. Therefore, the performance of employees is very important in achieving that goal. The purpose of this study was to analyze the influence of ability, effort, and support the company towards the employee's performance CV Gloria Konveksindo Clothing. The method of analysis used in this study is descriptive, Pearson Regression, and Multiple Regression. Data obtained from the appraisal of employ...

  4. Cyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa.

    Science.gov (United States)

    Garigliany, Mutien-Marie; Hagen, Ralf Matthias; Frickmann, Hagen; May, Jürgen; Schwarz, Norbert Georg; Perse, Amanda; Jöst, Hanna; Börstler, Jessica; Shahhosseini, Nariman; Desmecht, Daniel; Mbunkah, Herbert Afegenwi; Daniel, Achukwi Mbunkah; Kingsley, Manchang Tanyi; Campos, Renata de Mendonca; de Paula, Vanessa Salete; Randriamampionona, Njary; Poppert, Sven; Tannich, Egbert; Rakotozandrindrainy, Raphael; Cadar, Daniel; Schmidt-Chanasit, Jonas

    2014-12-18

    Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown to be restricted to central and southern Vietnam. Here we report the detection of CyCV-VN species in stool samples from pigs and humans from Africa, far beyond their supposed limited geographic distribution.

  5. Characterization of a novel gene encoding ankyrin repeat domain from Cotesia vestalis polydnavirus (CvBV)

    International Nuclear Information System (INIS)

    Shi Min; Chen Yafeng; Huang Fang; Liu Pengcheng; Zhou Xueping; Chen Xuexin

    2008-01-01

    Cotesia vestalis (Haliday) is an endoparasitoid of Plutella xylostella (L.) larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we describe the characterization of a gene (CvBV805) and its products. CvBV805 is located on the segment S8 of CvBV genome; it has a size of 909 bp and encodes a predicted protein of 125 amino acids. This protein contains an ankyrin repeat domain with a high degree of similarity with IκB-like genes. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected through 24 h. Tissue-specific expression patterns showed that CvBV805 might be involved in early host immunosuppression. CvBV805 was detected in parasitized hosts at 12 h p.p. and in rBac-eGFP-CvBV805-infected Tn-5B1-4 cells at 72 h.p.i. by using western blots analysis. The size of the protein expressed in the host hemocytes and infected Tn-5B1-4 cells was 17 kDa and 56 kDa (including eGFP), respectively, which nearly corresponded with the predicted molecular weight (14.31 kDa) of CvBV805, suggesting that the protein did not undergo extensive post-translational modification. The protein was confirmed to be present within the nuclear region in hemocytes of the parasitized P. xylostella larvae at 48 h p.p. using confocal laser scanning microscopy

  6. Evaluation of faba beans ( Vicia faba cv. Fiord) and sweet lupins ...

    African Journals Online (AJOL)

    Evaluation of faba beans ( Vicia faba cv. Fiord) and sweet lupins ( Lupizus albus cv. Kiev) as protein sources for ... P igsi n the metabolisrna ndN balance trial consurned5 % less( P < 0.01)o 1'thel upind ietc omparedto the fabab eand iet (l 370 vs. | 440 gld).N o significandt ift'erenceisn the digestiblee nergy( DE) contenta nd ...

  7. Pengaruh Kualitas Pelayanan terhadap Loyalitas Pelanggan di Rental Mobil CV. Ilham Motor Gandu Mlarak Ponorogo

    OpenAIRE

    Dhika Amalia

    2015-01-01

    The purpose of this study was to determine the amount of quality customer service and loyalty to know a very dominant factor affecting customer loyalty in the CV . Ilham Motor. This research method is the primary data from the questionnaires , sampling technique is a technique associated with customer 100 CV Ilham Motor . Data analysis is a quantitative method to test the validity , reliability testing , multiple linear regression analysis , the correlation coefficient analytic , analytic det...

  8. Lie groups and grand unified theories

    International Nuclear Information System (INIS)

    Gubitoso, M.D.

    1987-01-01

    This work presents some concepts in group theory and Lie algebras and, at same time, shows a method to study and work with semisimple Lie groups, based on Dynkin diagrams. The aproach taken is not completely formal, but it presents the main points of the elaboration of the method, so its mathematical basis is designed with the purpose of making the reading not so cumbersome to those who are interested only in a general picture of the method and its usefulness. At the end it is shown a brief review of gauge theories and two grand-unification models based on SO(13) and E 7 gauge groups. (author) [pt

  9. Grand Canonical Ensembles in General Relativity

    International Nuclear Information System (INIS)

    Klein, David; Yang, Wei-Shih

    2012-01-01

    We develop a formalism for general relativistic, grand canonical ensembles in space-times with timelike Killing fields. Using that, we derive ideal gas laws, and show how they depend on the geometry of the particular space-times. A systematic method for calculating Newtonian limits is given for a class of these space-times, which is illustrated for Kerr space-time. In addition, we prove uniqueness of the infinite volume Gibbs measure, and absence of phase transitions for a class of interaction potentials in anti-de Sitter space.

  10. Produccion de plantas grandes usando minicontenedores

    Science.gov (United States)

    R. Kasten Dumroese; Thomas D. Landis

    2012-01-01

    En América del Norte hay cada vez más interés por la producción híbrida o mixta. La misma consiste en cultivar plantines en contenedores de pequeño volumen y luego trasplantarlos; el trasplante se puede realizar a canteros en el suelo como en la producción a raíz desnuda o bien a contenedores más grandes. Originalmente se llamaban plantines "plug+", "...

  11. [Habitat factor analysis for Torreya grandis cv. Merrillii based on spatial information technology].

    Science.gov (United States)

    Wang, Xiao-ming; Wang, Ke; Ao, Wei-jiu; Deng, Jin-song; Han, Ning; Zhu, Xiao-yun

    2008-11-01

    Torreya grandis cv. Merrillii, a tertiary survival plant, is a rare tree species of significant economic value and expands rapidly in China. Its special habitat factor analysis has the potential value to provide guide information for its planting, management, and sustainable development, because the suitable growth conditions for this tree species are special and strict. In this paper, the special habitat factors for T. grandis cv. Merrillii in its core region, i.e., in seven villages of Zhuji City, Zhejiang Province were analyzed with Principal Component Analysis (PCA) and a series of data, such as IKONOS image, Digital Elevation Model (DEM), and field survey data supported by the spatial information technology. The results showed that T. grandis cv. Merrillii exhibited high selectivity of environmental factors such as elevation, slope, and aspect. 96.22% of T. grandis cv. Merrillii trees were located at the elevation from 300 to 600 m, 97.52% of them were found to present on the areas whose slope was less than 300, and 74.43% of them distributed on sunny and half-sunny slopes. The results of PCA analysis indicated that the main environmental factors affecting the habitat of T. grandis cv. Merrillii were moisture, heat, and soil nutrients, and moisture might be one of the most important ecological factors for T. grandis cv. Merrillii due to the unique biological and ecological characteristics of the tree species.

  12. The Grand Duchy on the Grand Tour: A Historical Study of Student Migration in Luxembourg

    Science.gov (United States)

    Rohstock, Anne; Schreiber, Catherina

    2013-01-01

    Since Luxembourg became independent in 1839, practically the entire political, economic and intellectual elite of the country has been socialised abroad. It was only in 2003 that the Grand Duchy set up its own university; before then, young Luxembourgers had to study in foreign countries. Over the past 150 years, Luxembourg has thus experienced…

  13. Efeitos da quitosana no desenvolvimento in vitro de videiras cv. merlot e no crescimento micelial do fungo Elsinoe ampelina Effects of the chitosan on the development of grapevines cv. Merlot and on the mycelial growth of the fungus Elsinoe ampelina

    Directory of Open Access Journals (Sweden)

    Aline José Maia

    2010-12-01

    Full Text Available Objetivou-se, neste trabalho, avaliar o efeito da quitosana no desenvolvimento in vitro de plântulas de videira cv. Merlot e sua atividade antifúngica sobre Elsinoe ampelina. No primeiro experimento, explantes da cultivar Merlot foram transferidos para meio de cultura DSD1, acrescido das concentrações 0; 25; 50,100; 150 e 200 mg L-1 de quitosana. Após 90 dias de cultivo in vitro, as plântulas foram avaliadas quanto ao número de raízes e de folhas, porcentagem de enraizamento e brotação, comprimento de raízes e de parte aérea, massa fresca da planta. No segundo experimento, incorporou-se às concentrações 0, 60, 120, 180, 240 e 300 mg L-1 de quitosana ao meio BDA, onde inoculou-se o fungo. Posteriormente, avaliou-se o crescimento micelial aos 6 e 9 dias de incubação a 25º C no escuro. No primeiro experimento para as variáveis comprimento médio da parte aérea, massa fresca da planta inteira, porcentagem de enraizamento e porcentagem de estacas brotadas houve decréscimo linear em função das concentrações de quitosana. No segundo experimento, houve efeito linear negativo em função das concentrações crescentes de quitosana, sendo que a inibição do crescimento micelial foi de 81,7%, demonstrando o grande potencial do uso de quitosana no controle da antracnose da videira.The objective of this research was to evaluate the effects of the chitosan on the development in vitro plantlets of grapevines cv. Merlot and its antifungal action on Elsinoe ampelina. In the first trial, explants of the grapevine cv. Merlot were transferred to growing medium DSD1 plus concentrations of 0; 25; 50; 100; 150 and 200 mg L-1 of the product. After 90 days of in vitro cultivation the plantlets were evaluated for root and leaf number, root and shoot length and fresh mass of roots and shoots. In the second experiment, doses of 0; 60; 120; 180; 240 and 300 mg L-1 were incorporated to the BDA media, where the fungus was inoculated. The mycelial

  14. 28 milliards d’euros, c’est grand comment ?

    OpenAIRE

    Ozer, Pierre; Salmon, Marc; Theunissen, Yannick

    2009-01-01

    J’ouvre mon quotidien. Fortis : 28 milliards d’euros de pertes en 2008. Ma fille de sept ans qui commence à lire et pose sans cesse des questions sur tout me lance : « Dis-moi, papa : 28 milliards d’euros, c’est grand comment ? ». Grande question… Je réfléchis et tente de trouver une parade parlante pour un enfant qui, in fine, se pose les mêmes questions que les grandes personnes.

  15. Gamma ray and neutrino detector facility (GRANDE), Task C

    International Nuclear Information System (INIS)

    Sobel, H.W.; Yodh, G.B.

    1991-08-01

    GRANDE is an imaging, water Cerenkov detector, which combines in one facility an extensive air shower array and a high-energy neutrino detector. We proposed that the detector be constructed in phases, beginning with an active detector area of 31,000 m 2 (GRANDE-I) 2 and expanding to a final size of 100,000--150,00 m 2 . Some of the characteristics of GRANDE-I are discussed in this paper

  16. Quantum corrections to ward identities of chronological AVV- and AAA-current correlators for nondegenerate many-fermion systems in the four-dimensional world

    International Nuclear Information System (INIS)

    Kucheryavij, V.Yi.

    1994-01-01

    The explicit form of nontrivial quantum corrections to Ward identities for AVV- and AAA-current correlators in the four-dimensional world for nondegenerate many-fermion systems of general type is obtained. The characteristics of all nontrivial quantum corrections for nondegenerate two-flavour fermion systems are classified and described. In particular, the well-known results follow from ours for the trivial quantum corrections (anomalies) in the case of the degenerate spectrum of fermion masses

  17. SO(10) supersymmetric grand unified theories

    Science.gov (United States)

    Dermisek, Radovan

    The origin of the fermion mass hierarchy is one of the most challenging problems in elementary particle physics. In the standard model fermion masses and mixing angles are free parameters. Supersymmetric grand unified theories provide a beautiful framework for physics beyond the standard model. In addition to gauge coupling unification these theories provide relations between quark and lepton masses within families, and with additional family symmetry the hierarchy between families can be generated. We present a predictive SO(10) supersymmetric grand unified model with D 3 x U(1) family symmetry. The hierarchy in fermion masses is generated by the family symmetry breaking D 3 x U(1) → ZN → nothing. This model fits the low energy data in the charged fermion sector quite well. We discuss the prediction of this model for the proton lifetime in light of recent SuperKamiokande results and present a clear picture of the allowed spectra of supersymmetric particles. Finally, the detailed discussion of the Yukawa coupling unification of the third generation particles is provided. We find a narrow region is consistent with t, b, tau Yukawa unification for mu > 0 (suggested by b → sgamma and the anomalous magnetic moment of the muon) with A0 ˜ -1.9m16, m10 ˜ 1.4m16, m16 ≳ 1200 GeV and mu, M1/2 ˜ 100--500 GeV. Demanding Yukawa unification thus makes definite predictions for Higgs and sparticle masses.

  18. O conservadorismo patronal da grande imprensa brasileira

    Directory of Open Access Journals (Sweden)

    Fonseca Francisco

    2003-01-01

    Full Text Available Através da análise da opinião dos editoriais dos quatro principais periódicos diários da grande imprensa, isto é, o Jornal do Brasil, O Globo, a Folha de S. Paulo e O Estado de S. Paulo - aqui considerados "aparelhos privados de hegemonia" -, este artigo procura desvendar os posicionamentos adotados perante a ordem social na Constituinte de 1987/1988, que também contribuem para compreender a reação à Consolidação das Leis do Trabalho. Observa-se, além do mais, as estratégias utilizadas para sua consecução. Conclui-se que, por mecanismos diversos, a grande imprensa contribuiu decisivamente para a introdução da agenda ideológica neoliberal no país, pois atuou de forma a "divulgar e vulgarizar" as idéias pertinentes a este ideário e de forma militantemente conservadora e patronal.

  19. Aprovechamiento de Salto Grande Argentina – Uruguay

    Directory of Open Access Journals (Sweden)

    Bongiovanni, M.

    1979-12-01

    Full Text Available The power harnessing of Salto Grande will be the first common project carried out by two South American Countries and this will foster greater integration of both countries. The project involves the following: — One 39 m-high dam — Two hydroelectric power plants — One international bridge — One shipping canal The benefits to be obtained from the project include: increase and improvement of the waterway network; increase of electricity production; railways and highway tie-ups between both countries; industrial development; increased availability of irrigation water; new tourist resorts; increase of fishing resources.

    El aprovechamiento hidroeléctrico de Salto Grande será el primer aprovechamiento común a dos países sudamericanos, lo que permitirá una mayor integración entre ellos. Consta de las siguientes obras: _ una presa de 39 m de altura; _ dos centrales hidroeléctricas; _ un puente internacional; _ un canal de navegación, etc. Entre los beneficios que se obtendrán destacan: aumento y mejora de la red de navegación; aumento de la producción eléctrica; enlace de las vías de ferrocarril y carreteras entre los dos países; desarrollo industrial; aumento de la disponibilidad de agua de riego; creación de zonas de turismo, e incremento de la riqueza piscícola.

  20. Elements in nucleotide sensing and hydrolysis of the AAA+ disaggregation machine ClpB: a structure-based mechanistic dissection of a molecular motor

    Energy Technology Data Exchange (ETDEWEB)

    Zeymer, Cathleen, E-mail: cathleen.zeymer@mpimf-heidelberg.mpg.de; Barends, Thomas R. M.; Werbeck, Nicolas D.; Schlichting, Ilme; Reinstein, Jochen, E-mail: cathleen.zeymer@mpimf-heidelberg.mpg.de [Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg (Germany)

    2014-02-01

    High-resolution crystal structures together with mutational analysis and transient kinetics experiments were utilized to understand nucleotide sensing and the regulation of the ATPase cycle in an AAA+ molecular motor. ATPases of the AAA+ superfamily are large oligomeric molecular machines that remodel their substrates by converting the energy from ATP hydrolysis into mechanical force. This study focuses on the molecular chaperone ClpB, the bacterial homologue of Hsp104, which reactivates aggregated proteins under cellular stress conditions. Based on high-resolution crystal structures in different nucleotide states, mutational analysis and nucleotide-binding kinetics experiments, the ATPase cycle of the C-terminal nucleotide-binding domain (NBD2), one of the motor subunits of this AAA+ disaggregation machine, is dissected mechanistically. The results provide insights into nucleotide sensing, explaining how the conserved sensor 2 motif contributes to the discrimination between ADP and ATP binding. Furthermore, the role of a conserved active-site arginine (Arg621), which controls binding of the essential Mg{sup 2+} ion, is described. Finally, a hypothesis is presented as to how the ATPase activity is regulated by a conformational switch that involves the essential Walker A lysine. In the proposed model, an unusual side-chain conformation of this highly conserved residue stabilizes a catalytically inactive state, thereby avoiding unnecessary ATP hydrolysis.

  1. Elements in nucleotide sensing and hydrolysis of the AAA+ disaggregation machine ClpB: a structure-based mechanistic dissection of a molecular motor

    International Nuclear Information System (INIS)

    Zeymer, Cathleen; Barends, Thomas R. M.; Werbeck, Nicolas D.; Schlichting, Ilme; Reinstein, Jochen

    2014-01-01

    High-resolution crystal structures together with mutational analysis and transient kinetics experiments were utilized to understand nucleotide sensing and the regulation of the ATPase cycle in an AAA+ molecular motor. ATPases of the AAA+ superfamily are large oligomeric molecular machines that remodel their substrates by converting the energy from ATP hydrolysis into mechanical force. This study focuses on the molecular chaperone ClpB, the bacterial homologue of Hsp104, which reactivates aggregated proteins under cellular stress conditions. Based on high-resolution crystal structures in different nucleotide states, mutational analysis and nucleotide-binding kinetics experiments, the ATPase cycle of the C-terminal nucleotide-binding domain (NBD2), one of the motor subunits of this AAA+ disaggregation machine, is dissected mechanistically. The results provide insights into nucleotide sensing, explaining how the conserved sensor 2 motif contributes to the discrimination between ADP and ATP binding. Furthermore, the role of a conserved active-site arginine (Arg621), which controls binding of the essential Mg 2+ ion, is described. Finally, a hypothesis is presented as to how the ATPase activity is regulated by a conformational switch that involves the essential Walker A lysine. In the proposed model, an unusual side-chain conformation of this highly conserved residue stabilizes a catalytically inactive state, thereby avoiding unnecessary ATP hydrolysis

  2. In-situ monitoring of blood glucose level for dialysis machine by AAA-battery-size ATR Fourier spectroscopy

    Science.gov (United States)

    Hosono, Satsuki; Sato, Shun; Ishida, Akane; Suzuki, Yo; Inohara, Daichi; Nogo, Kosuke; Abeygunawardhana, Pradeep K.; Suzuki, Satoru; Nishiyama, Akira; Wada, Kenji; Ishimaru, Ichiro

    2015-07-01

    For blood glucose level measurement of dialysis machines, we proposed AAA-battery-size ATR (Attenuated total reflection) Fourier spectroscopy in middle infrared light region. The proposed one-shot Fourier spectroscopic imaging is a near-common path and spatial phase-shift interferometer with high time resolution. Because numerous number of spectral data that is 60 (= camera frame rare e.g. 60[Hz]) multiplied by pixel number could be obtained in 1[sec.], statistical-averaging improvement realize high-accurate spectral measurement. We evaluated the quantitative accuracy of our proposed method for measuring glucose concentration in near-infrared light region with liquid cells. We confirmed that absorbance at 1600[nm] had high correlations with glucose concentrations (correlation coefficient: 0.92). But to measure whole-blood, complex light phenomenon caused from red blood cells, that is scattering and multiple reflection or so, deteriorate spectral data. Thus, we also proposed the ultrasound-assisted spectroscopic imaging that traps particles at standing-wave node. Thus, if ATR prism is oscillated mechanically, anti-node area is generated around evanescent light field on prism surface. By elimination complex light phenomenon of red blood cells, glucose concentration in whole-blood will be quantify with high accuracy. In this report, we successfully trapped red blood cells in normal saline solution with ultrasonic standing wave (frequency: 2[MHz]).

  3. The Pch2 AAA+ ATPase promotes phosphorylation of the Hop1 meiotic checkpoint adaptor in response to synaptonemal complex defects.

    Science.gov (United States)

    Herruzo, Esther; Ontoso, David; González-Arranz, Sara; Cavero, Santiago; Lechuga, Ana; San-Segundo, Pedro A

    2016-09-19

    Meiotic cells possess surveillance mechanisms that monitor critical events such as recombination and chromosome synapsis. Meiotic defects resulting from the absence of the synaptonemal complex component Zip1 activate a meiosis-specific checkpoint network resulting in delayed or arrested meiotic progression. Pch2 is an evolutionarily conserved AAA+ ATPase required for the checkpoint-induced meiotic block in the zip1 mutant, where Pch2 is only detectable at the ribosomal DNA array (nucleolus). We describe here that high levels of the Hop1 protein, a checkpoint adaptor that localizes to chromosome axes, suppress the checkpoint defect of a zip1 pch2 mutant restoring Mek1 activity and meiotic cell cycle delay. We demonstrate that the critical role of Pch2 in this synapsis checkpoint is to sustain Mec1-dependent phosphorylation of Hop1 at threonine 318. We also show that the ATPase activity of Pch2 is essential for its checkpoint function and that ATP binding to Pch2 is required for its localization. Previous work has shown that Pch2 negatively regulates Hop1 chromosome abundance during unchallenged meiosis. Based on our results, we propose that, under checkpoint-inducing conditions, Pch2 also possesses a positive action on Hop1 promoting its phosphorylation and its proper distribution on unsynapsed chromosome axes. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  4. Evaluation of analgesic, anti-inflammatory, anti-depressant and anti-coagulant properties of Lactuca sativa (CV. Grand Rapids) plant tissues and cell suspension in rats.

    Science.gov (United States)

    Ismail, Hammad; Mirza, Bushra

    2015-06-27

    Lactuca sativa (lettuce) has been traditionally used for relieving pain, inflammation, stomach problems including indigestion and lack of appetite. Moreover, the therapeutic significance of L. sativa includes its anticonvulsant, sedative-hypnotic and antioxidant properties. In the present study, the MC (methanol and chloroform; 1:1) and aqueous extracts of seed and leaf along with cell suspension exudate were prepared. These extracts were explored for their analgesic, anti-inflammatory, antidepressant and anticoagulant effects by hot plate analgesic assay; carrageenan induced hind paw edema test, forced swimming test and capillary method for blood clotting respectively in a rat model. The results were analyzed using one-way Analysis of Variance (ANOVA) followed by Turkey multiple comparison test. Interestingly, the extracts and the cell suspension exudate showed dual inhibition by reducing pain and inflammation. The results indicated that the aqueous extracts of leaf exhibited highest analgesic and anti-inflammatory activities followed by leaf MC, cell suspension exudate, seed aqueous and seed MC extracts. The current findings show that aqueous and MC extracts of seed have the least immobility time in the forced swimming test, which could act as an anti-depressant on the central nervous system. The leaf extracts and cell suspension exudate also expressed moderate anti-depressant activities. In anticoagulant assay, the coagulation time of aspirin (positive control) and MC extract of leaf was comparable, suggesting strong anti-coagulant effect. Additionally, no abnormal behavior or lethality was observed in any animal tested. Taken together, L. sativa can potentially act as a strong herbal drug due to its multiple pharmaceutical effects and is therefore of interest in drug discovery and development of formulations.

  5. La masa de los grandes impactores

    Science.gov (United States)

    Parisi, M. G.; Brunini, A.

    Los planetas han sido formados fundamentalmente acretando masa a través de colisiones con planetesimales sólidos. La masa más grande de la distribución de planetesimales y las masas máxima y mínima de los impactores, han sido calculadas usando los valores actuales del período y de la inclinación de los planetas (Lissauer & Safronov 1991; Parisi & Brunini 1996). Recientes investigaciones han mostrado, que las órbitas de los planetas gigantes no han sufrido variaciones con el tiempo, siendo su movimiento regular durante su evolución a partir de la finalización de la etapa de acreción (Laskar 1990, 1994). Por lo tanto, la eccentricidad actual de los planetas gigantes se puede utilizar para imponer una cota máxima a las masas y velocidades orbitales de los grandes impactores. Mediante un simple modelo dinámico, y considerando lo arriba mencionado, obtenemos la cota superior para la masa del planetesimal más grande que impactó a cada planeta gigante al final de su etapa de acreción. El resultado más importante de este trabajo es la estimación de la masa máxima permitida para impactar a Júpiter, la cúal es ~ 1.136 × 10 -1, siendo en el caso de Neptuno ~ 3.99 × 10 -2 (expresada en unidades de la masa final de cada planeta). Además, fue posible obtener la velocidad orbital máxima permitida para los impactores como una función de su masa, para cada planeta. Las cotas obtenidas para la masa y velocidad de los impactores de Saturno y Urano (en unidades de la masa y velocidad final de cada planeta respectivamente) son casi las mismas que las obtenidas para Júpiter debido a que estos tres planetas poseen similar eccentricidad actual. Nuestros resultados están en buen acuerdo con los obtenidos por Lissauer & Safronov (1991). Estas cotas podrían ser utilizadas para obtener la distribución de planetesimales en el Sistema Solar primitivo.

  6. [Regularity of Clinical Application of Lianquan (CV 23) in Chinese Ancient Times According to Literature of Traditional Chinese Medicine].

    Science.gov (United States)

    Deng, Mei-Jun; Liu, Chun-Yan; Xie, Yu; Zhu, Jie-Bin; Xu, Zhen-Hua

    2018-03-25

    To summarize the regularity of application of Lianquan (CV 23) in clinical practice in Chinese ancient times through analysis of ancient traditional Chinese medical (TCM) literature. A total of 60 books involving CV 23 from the 1 156 ancient TCM books listed in the fifth edition of Encyclopedia of Traditional Chinese Medicine were collected by using CV 23 as the main keyword and "Sheben" "Benchi", and "Jieben" (the other names of CV 23 in TCM)as the supplementary keywords and analyzed systematically. A database was then constructed from the collected data, including the related types of disorders or symptoms, acupoint recipes, and methods of needling and moxibustion, contraindications, etc. A total of 196 articles related to the application of CV 23 from 60 ancient classical books were collected in accordance with the inclusive criteria. Among them, 155 articles are referred to the indications of CV 23, 35 to types of disorders such as asthma, cough, tongue swelling with difficulty in speaking, protracted tongue, acute contraction of tongue root, vomiting, spasm syndrome, stroke, aphtha, problems of mouth and teeth, throat problems, etc. of the internal medicine, surgery, pediatrics, and five-sense organs; 64 items are referred to the application of single CV 23, 91 to CV 23-included recipes containing 111 adjunct acupoints, and 78 to stimulation of CV 23 with acupuncture needle, moxibustion, pricking blood, and fire needle. Moreover, of the 111 adjunct acupoints, the most commonly used are Shaoshang (LU 11), Tiantu (CV 22), Hegu (LI 4), Yuye (EX-HN 13), Zhongchong (PC 9), etc. Lianquan (CV 23) is mainly used for glossopharyngeal problems chiefly by syndrome-meridian differentiation. The supplement of complementary acupoints or five-shu points in combination with CV 23 has a synergistic effect. Moxibustion (3 moxa- cones in general) is often employed, and the needling depth is usually about 7.5 mm. The common contraindication of CV 23 is severe tongue swelling.

  7. Conservação de tangerina cv. Clemenules utilizando diferentes recobrimentos = Conservation of Mandarins cv. Clemenules with different cuticles

    Directory of Open Access Journals (Sweden)

    Rosa de Oliveira Treptow

    2007-01-01

    Full Text Available O objetivo deste trabalho foi verificar os efeitos de diferentes recobrimentos e períodos de armazenamento na conservação de tangerinas Clemenules. Após a colheita, as frutas foram submetidas ao pré-resfriamento por 12 horas a 5ºC e em seguida, aos seguintes tratamentos: T1 testemunha; T2 filme de polietileno microperfurado; T3 filme de polietileno não perfurado; T4 cera de carnaúba a 50%, diluída em água; T5 cera de carnaúba a 100% (não diluída. As tangerinas foram armazenadas por 20, 40 e 60 dias em temperatura de 5ºC e UR de 90-95%, depois foram mantidas em temperatura de 15±1ºC e UR de 75-80%, durante quatro dias simulando a comercialização. Na colheita e após cada período de armazenamento, seguido de simulação decomercialização, foram avaliadas as variáveis: perda de massa; cor; sólidos solúveis (SS; acidez titulável (AT; relação SS/AT; podridões, distúrbios fisiológicos e características sensoriais. Amodificação da atmosfera reduziu a perda de massa, a incidência de distúrbios e podridões de tangerinas Clemenules durante o armazenamento. As frutas acondicionadas em filme de polietilenosem perfuração ou aquelas revestidas com cera sem diluição apresentaram sabor estranho e menor qualidade geral. Tangerinas ‘Clemenules’ acondicionadas em filme de polietileno microperfurado ecera diluída em 50% de água podem ser armazenadas durante 60 dias a 5ºC e comercializadas durante quatro dias a 15±1ºC sem comprometer a qualidade sensorial.The aim of this work was to evaluate the effects of different cuticles and storage periods on the conservation of mandarins cv. Clemenules. After harvest the fruits were kept at 5ºC during 12 hours. Then, they were submitted to the following treatments: T1 control; T2 micro perforated polyethylene bag; T3 non-perforated polyethylene bag; T4 carnauba wax at 50% in water; T5 carnauba wax at 100% (without dilution. The mandarins were stored for 20, 40 and 60 days at 5

  8. AAA and PBC calculation accuracy in the surface build-up region in tangential beam treatments. Phantom and breast case study with the Monte Carlo code PENELOPE

    International Nuclear Information System (INIS)

    Panettieri, Vanessa; Barsoum, Pierre; Westermark, Mathias; Brualla, Lorenzo; Lax, Ingmar

    2009-01-01

    Background and purpose: In tangential beam treatments accurate dose calculation of the absorbed dose in the build-up region is of major importance, in particular when the target has superficial extension close to the skin. In most analytical treatment planning systems (TPSs) calculations depend on the experimental measurements introduced by the user in which accuracy might be limited by the type of detector employed to perform them. To quantify the discrepancy between analytically calculated and delivered dose in the build-up region, near the skin of a patient, independent Monte Carlo (MC) simulations using the PENELOPE code were performed. Dose distributions obtained with MC simulations were compared with those given by the Pencil Beam Convolution (PBC) algorithm and the Analytical Anisotropic Algorithm (AAA) implemented in the commercial TPS Eclipse. Material and methods: A cylindrical phantom was used to approximate the breast contour of a patient for MC simulations and the TPS. Calculations of the absorbed doses were performed for 6 and 18 MV beams for four different angles of incidence: 15 deg., 30 deg., 45 deg. and 75 deg. and different field sizes: 3 x 3 cm 2 , 10 x 10 cm 2 and 40 x 40 cm 2 . Absorbed doses along the phantom central axis were obtained with both the PBC algorithm and the AAA and compared to those estimated by the MC simulations. Additionally, a breast patient case was calculated with two opposed 6 MV photon beams using all the aforementioned analytical and stochastic algorithms. Results: For the 6 MV photon beam in the phantom case, both the PBC algorithm and the AAA tend to underestimate the absorbed dose in the build-up region in comparison to MC results. These differences are clinically irrelevant and are included in a 1 mm range. This tendency is also confirmed in the breast patient case. For the 18 MV beam the PBC algorithm underestimates the absorbed dose with respect to the AAA. In comparison to MC simulations the PBC algorithm tends

  9. Experimental verification of the Acuros XB and AAA dose calculation adjacent to heterogeneous media for IMRT and RapidArc of nasopharygeal carcinoma.

    Science.gov (United States)

    Kan, Monica W K; Leung, Lucullus H T; So, Ronald W K; Yu, Peter K N

    2013-03-01

    To compare the doses calculated by the Acuros XB (AXB) algorithm and analytical anisotropic algorithm (AAA) with experimentally measured data adjacent to and within heterogeneous medium using intensity modulated radiation therapy (IMRT) and RapidArc(®) (RA) volumetric arc therapy plans for nasopharygeal carcinoma (NPC). Two-dimensional dose distribution immediately adjacent to both air and bone inserts of a rectangular tissue equivalent phantom irradiated using IMRT and RA plans for NPC cases were measured with GafChromic(®) EBT3 films. Doses near and within the nasopharygeal (NP) region of an anthropomorphic phantom containing heterogeneous medium were also measured with thermoluminescent dosimeters (TLD) and EBT3 films. The measured data were then compared with the data calculated by AAA and AXB. For AXB, dose calculations were performed using both dose-to-medium (AXB_Dm) and dose-to-water (AXB_Dw) options. Furthermore, target dose differences between AAA and AXB were analyzed for the corresponding real patients. The comparison of real patient plans was performed by stratifying the targets into components of different densities, including tissue, bone, and air. For the verification of planar dose distribution adjacent to air and bone using the rectangular phantom, the percentages of pixels that passed the gamma analysis with the ± 3%/3mm criteria were 98.7%, 99.5%, and 97.7% on the axial plane for AAA, AXB_Dm, and AXB_Dw, respectively, averaged over all IMRT and RA plans, while they were 97.6%, 98.2%, and 97.7%, respectively, on the coronal plane. For the verification of planar dose distribution within the NP region of the anthropomorphic phantom, the percentages of pixels that passed the gamma analysis with the ± 3%/3mm criteria were 95.1%, 91.3%, and 99.0% for AAA, AXB_Dm, and AXB_Dw, respectively, averaged over all IMRT and RA plans. Within the NP region where air and bone were present, the film measurements represented the dose close to unit density water

  10. Experimental verification of the Acuros XB and AAA dose calculation adjacent to heterogeneous media for IMRT and RapidArc of nasopharygeal carcinoma

    International Nuclear Information System (INIS)

    Kan, Monica W. K.; Leung, Lucullus H. T.; So, Ronald W. K.; Yu, Peter K. N.

    2013-01-01

    Purpose: To compare the doses calculated by the Acuros XB (AXB) algorithm and analytical anisotropic algorithm (AAA) with experimentally measured data adjacent to and within heterogeneous medium using intensity modulated radiation therapy (IMRT) and RapidArc ® (RA) volumetric arc therapy plans for nasopharygeal carcinoma (NPC). Methods: Two-dimensional dose distribution immediately adjacent to both air and bone inserts of a rectangular tissue equivalent phantom irradiated using IMRT and RA plans for NPC cases were measured with GafChromic ® EBT3 films. Doses near and within the nasopharygeal (NP) region of an anthropomorphic phantom containing heterogeneous medium were also measured with thermoluminescent dosimeters (TLD) and EBT3 films. The measured data were then compared with the data calculated by AAA and AXB. For AXB, dose calculations were performed using both dose-to-medium (AXB Dm ) and dose-to-water (AXB Dw ) options. Furthermore, target dose differences between AAA and AXB were analyzed for the corresponding real patients. The comparison of real patient plans was performed by stratifying the targets into components of different densities, including tissue, bone, and air. Results: For the verification of planar dose distribution adjacent to air and bone using the rectangular phantom, the percentages of pixels that passed the gamma analysis with the ± 3%/3mm criteria were 98.7%, 99.5%, and 97.7% on the axial plane for AAA, AXB Dm , and AXB Dw , respectively, averaged over all IMRT and RA plans, while they were 97.6%, 98.2%, and 97.7%, respectively, on the coronal plane. For the verification of planar dose distribution within the NP region of the anthropomorphic phantom, the percentages of pixels that passed the gamma analysis with the ± 3%/3mm criteria were 95.1%, 91.3%, and 99.0% for AAA, AXB Dm , and AXB Dw , respectively, averaged over all IMRT and RA plans. Within the NP region where air and bone were present, the film measurements represented the

  11. Produtividade e valor nutritivo da Brachiaria brizantha cv. Marandu em um sistema silvipastoril Productivity and nutritional value of Brachiaria brizantha cv. Marandu in a silvopastoral system

    Directory of Open Access Journals (Sweden)

    L.F. Sousa

    2007-08-01

    Full Text Available O experimento foi realizado em sistema silvipastoril (Ssp localizado no bioma de Cerrado, no município de Lagoa Santa, Minas Gerais, no ano agrícola de 2004-2005. Avaliou-se a influência do Ssp composto pela arbórea bolsa-de-pastor (Zeyheria tuberculosa sobre os parâmetros produção, atributos morfogênicos, composição bromatológica e valor nutritivo da gramínea Brachiaria brizantha cv. Marandu, e os efeitos sobre as condições microclimáticas. O delineamento experimental foi em blocos ao acaso, com cinco blocos (épocas de corte, dois tratamentos (T1 - B. brizantha cv. Marandu sombreada com a Z. tuberculosa e T2 - B. brizantha cv. Marandu, a pleno sol e três repetições (parcelas. O sombreamento proporcionado pela Z. tuberculosa, apesar de reduzir a produção de matéria seca (MS da gramínea, favoreceu o aumento do teor de proteina bruta (PB e não alterou a produção de PB/ha e nem afetou a concentração de FDN. Os teores de FDA foram mais altos que os encontrados a pleno sol, o que provavelmente resultou em menor degradabilidade in vitro da MS e da matéria orgânica, não alterando a degradabilidade efetiva.The experiment was carried out in a Silvopastoral system (Sps located in Brazilian Savannah, Lagoa Santa county, Minas Gerais State, during the agricultural year of 2004-2005. It was studied the influence of Sps formed by "Bolsa de Pastor" (Zeyheria tuberculosa on the production, morphogenic attributes, bromatological composition and nutritional value of the graminaceous Brachiaria brizantha cv. Marandu as well as on the microclimatic aspects. The Sps was established in 1982, in a red-yellow latossol by the natural regeneration process. The experiment was based on a randomized block design, with 5 blocks (cutting times, 2 treatments (T1 - B. brizantha cv. Marandu shaded by Z. tuberculosa and T2 - B. brizantha cv. Marandu at open sky and three repetitions (plots. Although the DM production of the graminaceous in the Sps

  12. Local grand unification and string theory

    International Nuclear Information System (INIS)

    Nilles, Hans Peter; Vaudrevange, Patrick K.S.

    2009-09-01

    The low energy effective action of string theory depends strongly on the process of compactification and the localization of fields in extra dimensions. Explicit string constructions towards the minimal supersymmetric standard model (MSSM) reveal interesting results leading to the concept of local grand unification. Properties of the MSSM indicate that we might live at a special location close to an orbifold fixed point rather than a generic point in Calabi-Yau moduli space. We observe an enhancement of (discrete) symmetries that have various implications for the properties of the MSSM such as proton stability as well as solutions to the flavor problem, the m-problem and the strong CP-problem. (orig.)

  13. A unified grand tour of theoretical physics

    CERN Document Server

    Lawrie, Ian D

    2013-01-01

    A Unified Grand Tour of Theoretical Physics invites its readers to a guided exploration of the theoretical ideas that shape our contemporary understanding of the physical world at the fundamental level. Its central themes, comprising space-time geometry and the general relativistic account of gravity, quantum field theory and the gauge theories of fundamental forces, and statistical mechanics and the theory of phase transitions, are developed in explicit mathematical detail, with an emphasis on conceptual understanding. Straightforward treatments of the standard models of particle physics and cosmology are supplemented with introductory accounts of more speculative theories, including supersymmetry and string theory. This third edition of the Tour includes a new chapter on quantum gravity, focusing on the approach known as Loop Quantum Gravity, while new sections provide extended discussions of topics that have become prominent in recent years, such as the Higgs boson, massive neutrinos, cosmological perturba...

  14. Introduction to Grand Unified Theories. 12

    International Nuclear Information System (INIS)

    Wali, K.C.

    1989-01-01

    This chapter presents an introduction into Grand Unified Theories. After a discussion of the general features to be expected in any such theory, and of the motivations for them, a detailed presentation of SU(5) theory is given. The group structures, particle multiplets, gauge and Higgs bosons are explained. The two stages of spontaneous symmetry breaking via the Higgs model, are calculated individually and in combination. Fermion mass matrices and relations between quark and lepton masses are derived. predictions of SU(5) theory, calculated using renormalization group methods, are derived. The chapter ends with discussions that bring together particle physics and cosmology, including the baryon asymmetry problem, phase transitions in the very early universe, and singularities like domain walls, vortex lines and monopoles. (author). 9 refs.; 4 figs.; 5 tabs

  15. Supersymmetric grand unified theories and cosmology

    International Nuclear Information System (INIS)

    Lazarides, G.; Shafi, Q.

    1983-01-01

    By examining the behavior of supersymmetric grand unified theories (GUT's) in the very early universe we find two classes of realistic models. In one of them supersymmetry is broken at or near superheavy GUT scale. The cosmological implications of such models are expected to be similar to those of nonsupersymmetric GUT's. In the second class of models, the superheavy GUT scale is related to the supersymmetry-breaking scale in the manner of Witten. Two types of cosmological scenarios appear possible in this case, either with or without an intermediate (new) inflationary phase. They can be experimentally distinguished, since the former predicts an absence and the latter an observable number density of superheavy monopoles. A mechanism for generating baryon asymmetry in such models is pointed out

  16. Pitié pour les grandes villes !

    Directory of Open Access Journals (Sweden)

    Jérôme Monnet

    1997-02-01

    Full Text Available Roger Caillois disait, en 1938, qu´il existe "une représentation de la grande ville, assez puissante sur les imaginations pour que jamais en pratique ne soit posée la question de son exactitude, créée de toute pièce par le livre, assez répandue néanmoins pour faire partie de l´atmosphère mentale collective et posséder par suite une certaine force de contrainte"(Le mythe et l´homme, p.156 [c´est lui qui souligne]. En 1996, la presse française a consacré dossiers et articles à "Habitat II...

  17. Preface to a GUT (Grand Unified Theory)

    International Nuclear Information System (INIS)

    Honig, W.

    1982-01-01

    A Grand Unified Theory (GUT) is proposed exhibiting relativistic invariance and based on a physical model for vacuum space consisting of the superposition of oppositely charged continuous fluids. Models for the photon, electron, neutrino, proton, etc., consist of separate unique variations in the relative densities of the fluids and their flow patterns. This GUT is also based on the use of transfinite axiomatic number forms and on a concept of metrical relativity which hopefully reconciles the many logical dichotomies in and between Special Relativity and Quantum Mechanics. These ideas result in a number of experimental proposals and predicted results which appear to be underivable from present paradigms, first among which is a physical model for the hidden variable of Quantum Mechanics. It is on these features that attention should rest. (Auth.)

  18. Higgsless grand unified theory breaking and trinification

    International Nuclear Information System (INIS)

    Carone, Christopher D.; Conroy, Justin M.

    2004-01-01

    Boundary conditions on an extra dimensional interval can be chosen to break bulk gauge symmetries and to reduce the rank of the gauge group. We consider this mechanism in models with gauge trinification. We determine the boundary conditions necessary to break the trinified gauge group directly down to that of the standard model. Working in an effective theory for the gauge-symmetry-breaking parameters on a boundary, we examine the limit in which the grand-unified theory-breaking-sector is Higgsless and show how one may obtain the low-energy particle content of the minimal supersymmetric standard model. We find that gauge unification is preserved in this scenario, and that the differential gauge coupling running is logarithmic above the scale of compactification. We compare the phenomenology of our model to that of four dimensional 'trinified' theories

  19. Grand unified models including extra Z bosons

    International Nuclear Information System (INIS)

    Li Tiezhong

    1989-01-01

    The grand unified theories (GUT) of the simple Lie groups including extra Z bosons are discussed. Under authors's hypothesis there are only SU 5+m SO 6+4n and E 6 groups. The general discussion of SU 5+m is given, then the SU 6 and SU 7 are considered. In SU 6 the 15+6 * +6 * fermion representations are used, which are not same as others in fermion content, Yukawa coupling and broken scales. A conception of clans of particles, which are not families, is suggested. These clans consist of extra Z bosons and the corresponding fermions of the scale. The all of fermions in the clans are down quarks except for the standard model which consists of Z bosons and 15 fermions, therefore, the spectrum of the hadrons which are composed of these down quarks are different from hadrons at present

  20. FORMULASI STRATEGI PENINGKATAN PRODUKSI DOMBA CV MITRA TANI FARM, CIAMPEA, BOGOR

    Directory of Open Access Journals (Sweden)

    Amalia Sholehana

    2014-04-01

    Full Text Available ABSTRACTThis research was conducted to formulate to increase the sheep production at CV Mitra Tani Farm (CV MT Farm, Ciampea, Bogor. The research was conducted using descriptive analysis by analyzing the problem experienced by CV. MitraTani Farm. Primary data and secondary data were gathered at this research. The research was also conducted using several analytical methods such as value chain mapping, internal factor evaluation (IFE and external factor evaluation (EFE matrix, SWOT and quantitative strategic planning matrix (QSPM. Based on the value chain analysis, the current production of sheep at CV MT farm is highly depending on the supply from its partners and internal farmers. According to the IFE and EFE results, the differences of each weighted values were respectively 2,120 and 0,686 so the position of the company in the SWOT matrix was situated at Quadrant I. Therefore the company needs to use a growth strategy. The QSPM sequenced the strategy priority as follow (1 increasing the number of lambs, (2 developing the management, (3 strengthening the capital and ownership, (4 improving the quality through technology. The result of the gross profit margin calculation, if the sheep‘s selling is increased up to 1000 sheep per month then the possible annual profit that can be earned by CV MT farm is 40,34% of the total production.Keywords: MT Farm, sheep production, strategies, SWOT, QSPM, gross profit marginABSTRAKPenelitian ini dilakukan untuk memformulasikan strategi peningkatan produksi domba di CV Mitra Tani Farm (CV MT Farm, Ciampea, Bogor. Pendekatan penelitian yang diterapkan adalah analisis deskriptif yang dilakukan dengan mempelajari permasalahan dari objek yang diteliti. Data yang dikumpulkan dalam penelitian ini adalah data primer dan data sekunder. Penelitian dilakukan menggunakan alat analisis berupa pemetaan rantai nilai,  matriks IFE (Internal Factor Evaluation dan EFE (External Factor Evaluation, SWOT, dan QSPM (Quantitative

  1. Evaluation of the Analytical Anisotropic Algorithm (AAA) in dose calculation for fields with non-uniform fluences considering heterogeneity correction; Avaliacao do Algoritmo Analitico Anisotropico (AAA) no calculo de dose para campos com fluencia nao uniforme considerando correcao de heterogeneidade

    Energy Technology Data Exchange (ETDEWEB)

    Bornatto, P.; Funchal, M.; Bruning, F.; Toledo, H.; Lyra, J.; Fernandes, T.; Toledo, F.; Marciao, C., E-mail: pricila_bornatto@yahoo.com.br [Hospital Erasto Gaertner (LPCC), Curitiba, PR (Brazil). Departamento de Radioterapia

    2014-08-15

    The purpose of this study is to evaluate the calculation of dose distribution AAA (Varian Medical Systems) for fields with non-uniform fluences considering heterogeneity correction. Five different phantoms were used with different density materials. These phantoms were scanned in the CT BrightSpeed (©GE Healthcare) upon the array of detectors MAPCHECK2 TM (Sun Nuclear Corporation) and irradiated in a linear accelerator 600 CD (Varian Medical Systems) 6MV and rate dose 400MU/min with isocentric setup. The fluences used were exported from IMRT plans, calculated by ECLIPSE™ planning system (Varian Medical Systems), and a 10x10 cm{sup 2} field to assess the heterogeneity correction for uniform fluence. The measured dose distribution was compared to the calculated by Gamma analysis with approval criteria of 3% / 3 mm and 10% threshold. The evaluation was performed using the software SNCPatient (Sun Nuclear Corporation) and considering absolute dose normalized at maximum. The phantoms best performers were those with low density materials, with an average of 99.2% approval. Already phantoms with plates of higher density material presented various fluences below 95% of the points approved. The average value reached 94.3%. It was observed a dependency between fluency and approved percentage points, whereas for the same fluency, 100% of the points have been approved in all phantoms. The approval criteria for IMRT plans recommended in most centers is 3% / 3mm with at least 95% of points approved, it can be concluded that, under these conditions, the IMRT plans with heterogeneity correction can be performed , however the quality control must be careful because the difficulty of the system to accurately predict the dose distribution in certain situations. (author)

  2. Review of freeboard: Grand Rapids Generating Station

    Energy Technology Data Exchange (ETDEWEB)

    Groeneveld, J.L.; Harding, W.B.; Bonin, D.V.; Fuchs, D.M. [Acres Manitoba Ltd., Winnipeg, MB (Canada); Warner, B.J. [Manitoba Hydro, Winnipeg, MB (Canada)

    2001-10-01

    Constructed during the period 1960-1965, the Grand Rapids Generating Station is a 472 MW hydroelectric station located approximately 400 kilometres northwest of Winnipeg, Manitoba, on the Saskatchewan River. An intake structure, four penstocks, a four-unit plus house unit powerhouse, wing walls, extensive dyke structures and a four-bay spillway are the components of the generating station. A little over ten years ago, the Manitoba Hydro Dam Safety Program was initiated. The program included a detailed dam safety review of the Grand rapids Generating Station. A potential deficiency in the freeboard allowance for several of the earthen dykes was revealed by the review process. The dam safety guidelines sponsored by the Canadian Dam Association were not met. The occurrence of a 1:1000 year wind event from the critical direction when the reservoir was at or near its full supply level was compounded by the analysis. The establishment of a wind and wave monitoring program was included in the deficiency studies commissioned. The objective was to confirm the empirical estimates concerning wave height, the development and usage of a two dimensional numerical wave model, and additional freeboard analyses to refine estimates of the recurrence interval of the current level of protection. A statistical Monte Carlo analysis was performed in the case of the estimates of the recurrence interval to determine the joint probabilities of seasonal variations in wind direction, wind speed, and reservoir level. The estimate of the actual risk of overtopping was therefore refined, and the details pertaining to the methodology and the conclusions of the analysis are all included in the paper. 15 refs., 4 tabs., 9 figs.

  3. Perspective view over the Grand Canyon, Arizona

    Science.gov (United States)

    2001-01-01

    This simulated true color perspective view over the Grand Canyon was created from Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) data acquired on May 12, 2000. The Grand Canyon Village is in the lower foreground; the Bright Angel Trail crosses the Tonto Platform, before dropping down to the Colorado Village and then to the Phantom Ranch (green area across the river). Bright Angel Canyon and the North Rim dominate the view. At the top center of the image the dark blue area with light blue haze is an active forest fire. ASTER is one of five Earth-observing instruments launched December 18, 1999, on NASA's Terra satellite. The instrument was built by Japan's Ministry of Economy, Trade and Industry. A joint U.S./Japan science team is responsible for validation and calibration of the instrument and the data products. The broad spectral coverage and high spectral resolution of ASTER provides scientists in numerous disciplines with critical information for surface mapping, and monitoring of dynamic conditions and temporal change. Example applications are: monitoring glacial advances and retreats; monitoring potentially active volcanoes; identifying crop stress; determining cloud morphology and physical properties; wetlands evaluation; thermal pollution monitoring; coral reef degradation; surface temperature mapping of soils and geology; and measuring surface heat balance. The U.S. science team is located at NASA's Jet Propulsion Laboratory, Pasadena, Calif. The Terra mission is part of NASA's Science Mission Directorate. Size: 5 km in foreground to 40 km Location: 36.3 degrees north latitude, 112 degrees west longitude Orientation: North-northeast at top Original Data Resolution: ASTER 15 meters Dates Acquired: May 12, 2000

  4. Phase transitions at finite chemical potential in grand unified theories

    International Nuclear Information System (INIS)

    Bailin, D.; Love, A.

    1984-01-01

    We discuss the circumstances in which non-zero chemical potentials might prevent symmetry restoration in phase transitions in the early universe at grand unification or partial unification scales. The general arguments are illustrated by consideration of SO(10) and SU(5) grand unified theories. (orig.)

  5. Fetal Outcomes among Grand Multiparous and Multiparous Women ...

    African Journals Online (AJOL)

    Objective: To compare fetal outcomes among grand multiparous (para 5-9) and multiparous (para 2-4) delivering in Mulago hospital, Uganda. Design: Prospective cohort study. Setting: Mulago hospital, Uganda. Subjects: One hundred and fifty six grand multiparous and multiparous women were recruited on admission in ...

  6. First report of Phytophthora ramorum infecting grand fir in California

    Science.gov (United States)

    K.L. Riley; G.A. Chastagner

    2011-01-01

    Phytophthora ramorum was detected on grand fir in 2003 and 2005 in a Christmas tree plantation near Los Gatos, CA, in association with infected California bay laurel. Isolates derived from stem lesions were used to inoculate grand fir seedlings in two tests. Isolations from lesions on inoculated plants were positive for P. ramorum...

  7. Plants, arthropods, and birds of the Rio Grande [chapter 7

    Science.gov (United States)

    Deborah M. Finch; Gale L. Wolters; Wang Yong; Mary Jean Mund

    1995-01-01

    Human populations have increased dramatically along the Rio Grande since European settlement. Human use of water for irrigation and consumption, and human use of land for agriculture, urban centers, livestock grazing, and recreation have changed Rio Grande ecosystems by altering flood cycles, channel geomorphology, upslope processes, and water quality and quantity....

  8. Research of the Rio Grande Ecosystem Management Program

    Science.gov (United States)

    Deborah M. Finch

    2000-01-01

    This paper describes the mission, objectives, and preliminary results of the Middle Rio Grande Ecosystem Management Research Program managed at the Rocky Mountain Research Station's Albuquerque laboratory. This program was initiated in 1994 to address growing pressures to effectively manage the limited resources of the middle Rio Grande Basin. The program is...

  9. Elastoplasticidad anisotropa de metales en grandes deformaciones

    Science.gov (United States)

    Caminero Torija, Miguel Angel

    El objetivo de este trabajo es el desarrollo de modelos y algoritmos numericos que simulen el comportamiento del material bajo estas condiciones en el contexto de programas de elementos finitos, dando como resultado predicciones mas precisas de los procesos de conformado y deformacion plastica en general. Para lograr este objetivo se han desarrollado diversas tareas destinadas a mejorar las predicciones en tres aspectos fundamentales. El primer aspecto consiste en la mejora de la descripcion del endurecimiento cinematico anisotropo en pequenas deformaciones, lo cual se ha realizado a traves de modelos y algoritmos implicitos de superficies multiples. Ha sido estudiada la consistencia de este tipo de modelos tanto si estan basados en una regla implicita similar a la de Mroz o en la regla de Prager. Ademas se han simulado los ensayos de Lamba y Sidebottom, obteniendo, en contra de la creencia general, muy buenas predicciones con la regla de Prager. Dichos modelos podrian ser extendidos de forma relativamente facil para considerar grandes deformaciones a traves de procedimientos en deformaciones logaritmicas, similares a los desarrollados en esta tesis y detallados a continuacion. El segundo aspecto consiste en la descripcion de la anisotropia elastoplastica inicial. Esto se ha conseguido mediante el desarrollo de modelos y algoritmos para plasticidad anisotropa en grandes deformaciones, bien ignorando la posible anisotropia elastica, bien considerandola simultaneamente con la anisotropia plastica. Para ello ha sido necesario desarrollar primero un nuevo algoritmo de elastoplasticidad anisotropa en pequenas deformaciones consistentemente linealizado y sin despreciar ningun termino, de tal forma que se conserve la convergencia cuadratica de los metodos de Newton. Este algoritmo en pequenas deformaciones ha servido para realizar la correccion plastica de dos algoritmos en grandes deformaciones. El primero de estos algoritmos es una variacion del clasico algoritmo de

  10. 3CV+2: modelo de calidad para la construcción de la vivienda 3CV+2: quality model for dwelling construction

    Directory of Open Access Journals (Sweden)

    Salvador García Rodríguez

    2008-08-01

    Full Text Available Este artículo presenta el modelo y los resultados que, desde Junio de 2005, se han alcanzado en el desarrollo del Programa Nacional de Certificación de los Procesos Constructivos de la Vivienda en base al Modelo 3cv+2. El programa se ha desarrollado con base a un convenio entre el Tecnológico de Monterrey y la Cámara Nacional de la Industria de Desarrollo y Promoción de Vivienda. Actualmente se encuentran voluntariamente en el programa 52 empresas inmobiliarias de diferentes estados de la República Mexicana. El modelo de calidad 3cv+2 es una metodología que busca establecer en los niveles operativos de la construcción criterios que permitan reducir la variabilidad del proceso de construcción, y además permita de manera sistemática, y en un proceso de mejora continua, evaluar el desempeño de calidad en proyectos de construcción de vivienda. El modelo 3cv+2 define en su nombre el alcance del mismo, 3 criterios de calidad para la vivienda (insumos, proceso y producto, apoyado por 2 auditorías (+2, la auditoría interna de la propia empresa, mas la auditoría externa hecha por el Tecnológico de Monterrey. El modelo 3cv+2 busca garantizar que los insumos utilizados en la construcción de las viviendas sean de calidad superior, y que los procesos de ejecución posean características de calidad, productividad, eficiencia, racionalización, y estandarización; de tal manera que insumos y productos sean de alto valor agregado, y garanticen la calidad especificada en las etapas parciales y en la vivienda terminada.This paper presents the model and the results that have been achieved during the development of the National Quality Certification Program based on the model 3cv +2 from June 2005. This program has been developed based on an agreement between the Monterrey Tech and the National Chamber of Housing Industry. Nowadays, the program has 52 voluntary real estate companies from different states of Mexico. The 3cv+2 quality model is a

  11. Studies of frequency dependent C-V characteristics of neutron irradiated p+-n silicon detectors

    International Nuclear Information System (INIS)

    Li, Zheng; Kraner, H.W.

    1990-10-01

    Frequency-dependent capacitance-voltage fluence (C-V) characteristics of neutron irradiated high resistivity silicon p + -n detectors have been observed up to a fluence of 8.0 x 10 12 n/cm 2 . It has been found that frequency dependence of the deviation of the C-V characteristic (from its normal V -1/2 dependence), is strongly dependent on the ratio of the defect density and the effective doping density N t /N' d . As the defect density approaches the effective dopant density, or N t /N' d → 1, the junction capacitance eventually assumes the value of the detector geometry capacitance at high frequencies (f ≤ 10 5 Hz), independent of voltage. A two-trap-level model using the concept of quasi-fermi levels has been developed, which predicts both the effects of C-V frequency dependence and dopant compensation observed in this study

  12. Immobilization of enzymatic extracts of Portulaca oleracea cv. roots for oxidizing aqueous bisphenol A.

    Science.gov (United States)

    Matsushima, Kazuki; Kaneda, Hirokazu; Harada, Kazuo; Matsuura, Hideyuki; Hirata, Kazumasa

    2015-05-01

    Water pollution from the release of industrial wastewater is a serious problem for almost every industry. Enzymes from portulaca, Portulaca oleracea cv., have been investigated for their ability to degrade bisphenol A (BPA), one of the well-known estrogenic pollutants. Enzymatic crude extracts from P. oleracea cv. roots were immobilized on aminopropyl-modified glass beads. They maintained BPA metabolic activity over a broad range of pH values and temperatures. The immobilized enzyme was reusable with more than 50 % of its initial activity retained after 12 batch reactions and no loss of activity after storage for 1 month at -30 °C. Thus, the immobilization of extracts from P. oleracea cv. roots is a useful method for removing BPA from industrial wastewater.

  13. Light equation in eclipsing binary CV Boo: third body candidate in elliptical orbit

    Science.gov (United States)

    Bogomazov, A. I.; Kozyreva, V. S.; Satovskii, B. L.; Krushevska, V. N.; Kuznyetsova, Y. G.; Ehgamberdiev, S. A.; Karimov, R. G.; Khalikova, A. V.; Ibrahimov, M. A.; Irsmambetova, T. R.; Tutukov, A. V.

    2016-12-01

    A short period eclipsing binary star CV Boo is tested for the possible existence of additional bodies in the system with a help of the light equation method. We use data on the moments of minima from the literature as well as from our observations during 2014 May-July. A variation of the CV Boo's orbital period is found with a period of {≈}75 d. This variation can be explained by the influence of a third star with a mass of {≈}0.4 M_{⊙} in an eccentric orbit with e≈0.9. A possibility that the orbital period changes on long time scales is discussed. The suggested tertiary companion is near the chaotic zone around the central binary, so CV Boo represents an interesting example to test its dynamical evolution. A list of 14 minima moments of the binary obtained from our observations is presented.

  14. Segmentation of kidney using C-V model and anatomy priors

    Science.gov (United States)

    Lu, Jinghua; Chen, Jie; Zhang, Juan; Yang, Wenjia

    2007-12-01

    This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and anatomy prior knowledge. In pre-processing stage, the candidate kidney regions are located. Then C-V model formulated by level set method is applied in these smaller ROI, which can reduce the calculation complexity to a certain extent. At last, after some mathematical morphology procedures, the specified kidney structures have been extracted interactively with prior knowledge. The satisfying results on abdominal CT series show that the proposed approach keeps all the advantages of C-V model and overcome its disadvantages.

  15. Isotopic coherence of refractory inclusions from CV and CK meteorites: Evidence from multiple isotope systems

    Science.gov (United States)

    Shollenberger, Quinn R.; Borg, Lars E.; Render, Jan; Ebert, Samuel; Bischoff, Addi; Russell, Sara S.; Brennecka, Gregory A.

    2018-05-01

    Calcium-aluminum-rich inclusions (CAIs) are the oldest dated materials in the Solar System and numerous previous studies have revealed nucleosynthetic anomalies relative to terrestrial rock standards in many isotopic systems. However, most of the isotopic data from CAIs has been limited to the Allende meteorite and a handful of other CV3 chondrites. To better constrain the isotopic composition of the CAI-forming region, we report the first Sr, Mo, Ba, Nd, and Sm isotopic compositions of two CAIs hosted in the CK3 desert meteorites NWA 4964 and NWA 6254 along with two CAIs from the CV3 desert meteorites NWA 6619 and NWA 6991. After consideration of neutron capture processes and the effects of hot-desert weathering, the Sr, Mo, Ba, Nd, and Sm stable isotopic compositions of the samples show clearly resolvable nucleosynthetic anomalies that are in agreement with previous results from Allende and other CV meteorites. The extent of neutron capture, as manifested by shifts in the observed 149Sm-150Sm isotopic composition of the CAIs is used to estimate the neutron fluence experienced by some of these samples and ranges from 8.40 × 1013 to 2.11 × 1015 n/cm2. Overall, regardless of CAI type or host meteorite, CAIs from CV and CK chondrites have similar nucleosynthetic anomalies within analytical uncertainty. We suggest the region that CV and CK CAIs formed was largely uniform with respect to Sr, Mo, Ba, Nd, and Sm isotopes when CAIs condensed and that CAIs hosted in CV and CK meteorites are derived from the same isotopic reservoir.

  16. Khatyrka, a new CV3 find from the Koryak Mountains, Eastern Russia

    Science.gov (United States)

    MacPherson, Glenn J.; Andronicos, Christopher L.; Bindi, Luca; Distler, Vadim V.; Eddy, Michael P.; Eiler, John M.; Guan, Yunbin; Hollister, Lincoln S.; Kostin, Alexander; Kryachko, Valery; Steinhardt, William M.; Yudovskaya, Marina; Steinhardt, Paul J.

    2013-08-01

    A new meteorite find, named Khatyrka, was recovered from eastern Siberia as a result of a search for naturally occurring quasicrystals. The meteorite occurs as clastic grains within postglacial clay-rich layers along the banks of a small stream in the Koryak Mountains, Chukotka Autonomous Okrug of far eastern Russia. Some of the grains are clearly chondritic and contain Type IA porphyritic olivine chondrules enclosed in matrices that have the characteristic platy olivine texture, matrix olivine composition, and mineralogy (olivine, pentlandite, nickel-rich iron-nickel metal, nepheline, and calcic pyroxene [diopside-hedenbergite solid solution]) of oxidized-subgroup CV3 chondrites. A few grains are fine-grained spinel-rich calcium-aluminum-rich inclusions with mineral oxygen isotopic compositions again typical of such objects in CV3 chondrites. The chondritic and CAI grains contain small fragments of metallic copper-aluminum-iron alloys that include the quasicrystalline phase icosahedrite. One grain is an achondritic intergrowth of Cu-Al metal alloys and forsteritic olivine ± diopsidic pyroxene, both of which have meteoritic (CV3-like) oxygen isotopic compositions. Finally, some grains consist almost entirely of metallic alloys of aluminum + copper ± iron. The Cu-Al-Fe metal alloys and the alloy-bearing achondrite clast are interpreted to be an accretionary component of what otherwise is a fairly normal CV3 (oxidized) chondrite. This association of CV3 chondritic grains with metallic copper-aluminum alloys makes Khatyrka a unique meteorite, perhaps best described as a complex CV3 (ox) breccia.

  17. Mineralogy and Petrography of MIL 090001, a Highly Altered CV Chondrite from the Reduced Sub-Group

    Science.gov (United States)

    Keller, Lindsay P.

    2011-01-01

    MIL 090001 is a large (greater than 6 kg) CV chondrite from the reduced subgroup (CV(sub red)) that was recovered during the 2009-2010 ANSMET field season [1]. The CV(sub red) subgroup meteorites retain primitive characteristics and have escaped the Na and Fe meta-somatism that affected the oxidized (CV(sub ox)) subgroups. MIL 090001 is, however, reported to be altered [1], and thus a major objective of this study is to characterize its mineralogy and petrography and the extent of the alteration.

  18. Detoxification of microcystin-LR in water by Portulaca oleracea cv.

    Science.gov (United States)

    Isobe, Takatoshi; Okuhata, Hiroshi; Miyasaka, Hitoshi; Jeon, Bong-Seok; Park, Ho-Dong

    2014-03-01

    Microcystin-LR (0.02 μg/ml) in the hydroculture medium of Portulaca oleracea cv., became below the detection level (<0.0001 μg/ml) by HPLC analysis after 7 days. The toxicity of microcystin estimated with protein phosphatase inhibition assay, however, remained at 37% of the initial level, indicating that microcystin-LR was transformed by P. oleracea cv. into unknown compound(s) of lower toxicity. Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  19. Albuquerque/Middle Rio Grande Urban Waters Viewer

    Science.gov (United States)

    These data have been compiled in support of the Middle Rio Grande/Albuquerque Urban Waters Partnership for the region including Albuquerque, New Mexico.The Middle Rio Grande/Albuquerque Urban Waters Federal Partnership is co-chaired by the U.S. Dept. of Housing and Urban Development and the U.S. Environmental Protection Agency. There are also a number of other federal agencies engaged in projects with Tribal, State, and local officials, and community stakeholders. Like many western river ecosystems, the Middle Rio Grande faces numerous challenges in balancing competing needs within a finite water supply and other resource constrains. Historical practices by our ancestors and immigrants to the Middle Rio Grande have established the conditions that we have inherited. Long-term drought exacerbated by climate change is changing conditions that affect natural and human communities as we strive to improve our precious Rio Grande.The Middle Rio Grande/Albuquerque Urban Waters Federal Partnership will reconnect our urban communities, particularly those that are overburdened or economically distressed, with the waterway by improving coordination among federal agencies and collaborating with community-led revitalization efforts. Our projects will improve our community water systems and promote their economic, environmental and social benefits. Specifically, the Middle Rio Grande/Albuquerque Urban Waters Federal Partnership will support the development of the Valle de Oro

  20. Grand Fir Nutrient Management in the Inland Northwestern USA

    Directory of Open Access Journals (Sweden)

    Dennis R. Parent

    2016-11-01

    Full Text Available Grand fir (Abies grandis (Douglas ex D. Don Lindley is widely distributed in the moist forests of the Inland Northwest. It has high potential productivity, its growth being nearly equal to western white pine, the most productive species in the region. There are large standing volumes of grand fir in the region. Nutritionally, the species has higher foliage cation concentrations than associated conifers, especially potassium (K and calcium (Ca. In contrast, it has lower nitrogen (N foliage concentrations, which creates favorable nutrient balance on N-limited sites. Despite concentration differences, grand fir stores proportionally more nutrients per tree than associated species because of greater crown biomass. Although few fertilization trials have examined grand fir specifically, its response is inferred from its occurrence in many monitored mixed conifer stands. Fertilization trials including grand fir either as a major or minor component show that it has a strong diameter and height growth response ranging from 15% to 50% depending in part on site moisture availability and soil geology. Grand fir tends to have a longer response duration than other inland conifers. When executed concurrently with thinning, fertilization often increases the total response. Late rotation application of N provides solid investment returns in carefully selected stands. Although there are still challenges with the post-fertilization effects on tree mortality, grand fir will continue to be an important species with good economic values and beneficial responses to fertilization and nutrient management.

  1. Effect of physiological harvest stages on the composition of bioactive compounds in Cavendish bananas*

    Science.gov (United States)

    Bruno Bonnet, Christelle; Hubert, Olivier; Mbeguie-A-Mbeguie, Didier; Pallet, Dominique; Hiol, Abel; Reynes, Max; Poucheret, Patrick

    2013-01-01

    The combined influence of maturation, ripening, and climate on the profile of bioactive compounds was studied in banana (Musa acuminata, AAA, Cavendish, cv. Grande Naine). Their bioactive compounds were determined by the Folin-Ciocalteu assay and high-performance thin layer chromatographic (HPTLC) method. The polyphenol content of bananas harvested after 400 degree days remained unchanged during ripening, while bananas harvested after 600 and 900 degree days exhibited a significant polyphenol increase. Although dopamine was the polyphenol with the highest concentration in banana peels during the green developmental stage and ripening, its kinetics differed from the total polyphenol profile. Our results showed that this matrix of choice (maturation, ripening, and climate) may allow selection of the banana (M. acuminata, AAA, Cavendish, cv. Grande Naine) status that will produce optimal concentrations of identified compounds with human health relevance. PMID:23549844

  2. PspF-binding domain PspA1-144 and the PspA·F complex: New insights into the coiled-coil-dependent regulation of AAA+ proteins.

    Science.gov (United States)

    Osadnik, Hendrik; Schöpfel, Michael; Heidrich, Eyleen; Mehner, Denise; Lilie, Hauke; Parthier, Christoph; Risselada, H Jelger; Grubmüller, Helmut; Stubbs, Milton T; Brüser, Thomas

    2015-11-01

    Phage shock protein A (PspA) belongs to the highy conserved PspA/IM30 family and is a key component of the stress inducible Psp system in Escherichia coli. One of its central roles is the regulatory interaction with the transcriptional activator of this system, the σ(54) enhancer-binding protein PspF, a member of the AAA+ protein family. The PspA/F regulatory system has been intensively studied and serves as a paradigm for AAA+ enzyme regulation by trans-acting factors. However, the molecular mechanism of how exactly PspA controls the activity of PspF and hence σ(54) -dependent expression of the psp genes is still unclear. To approach this question, we identified the minimal PspF-interacting domain of PspA, solved its structure, determined its affinity to PspF and the dissociation kinetics, identified residues that are potentially important for PspF regulation and analyzed effects of their mutation on PspF in vivo and in vitro. Our data indicate that several characteristics of AAA+ regulation in the PspA·F complex resemble those of the AAA+ unfoldase ClpB, with both proteins being regulated by a structurally highly conserved coiled-coil domain. The convergent evolution of both regulatory domains points to a general mechanism to control AAA+ activity for divergent physiologic tasks via coiled-coil domains. © 2015 John Wiley & Sons Ltd.

  3. Functional renal perfusion imaging with colour mapping: is it a useful adjunct to spiral CT of in the assessment of abdominal aortic aneurysm (AAA)?

    International Nuclear Information System (INIS)

    Blomley, Martin J.K.; McBride, Alan; Mohammedtagi, Sima; Albrecht, Thomas; Harvey, Christopher J.; Jaeger, Rolf; Standfield, Nigel J.; Dawson, Peter

    1999-01-01

    Aim: To ensure optimal timing with pre-operative spiral CT for abdominal aortic aneurysms (AAA), an initial 'timing' single level CT is commonly performed with a small bolus of contrast. This can be exploited to obtain adjunct functional information on renal perfusion. We have investigated the potential of this to measure renal perfusion, to produce colour renal perfusion maps and to predict surgical outcome in infrarenal aortic aneurysm assessment. Methods: We studied 21 patients being assessed for repair of infrarenal AAA. Prior to the spiral CT, a single level through the renal hili and aorta was scanned after the intravenous injection of 25 ml of contrast given at 10 ml/s. Ten 1 s duration scans were performed from 8 to 30 s after injection. Optimal timing for CT angiography can then be determined. Time-density curves were then drawn for both kidneys and aorta using regions of interest (ROIs) or pixel-by-pixel analysis. Renal cortical perfusion was measured using both ROI analysis and pseudocolour perfusion images. Following previous work, perfusion was calculated as the peak upslope of the tissue time density curve divided by peak aortic enhancement. Results: Cortical mean perfusion averaged 2.48 ml/min per ml (range 0.8-3.7 ml/min per ml n=34) and the values obtained agreed with literature expectations. Follow up in the 10 patients proceeding to AAA repair suggest low mean perfusion values and predict a raised postoperative creatinine (P<0.05) Conclusions: Additional functional data and imaging can be obtained from the initial timing scan of a CT study, without requiring a dedicated study

  4. DnaA protein DNA-binding domain binds to Hda protein to promote inter-AAA+ domain interaction involved in regulatory inactivation of DnaA.

    Science.gov (United States)

    Keyamura, Kenji; Katayama, Tsutomu

    2011-08-19

    Chromosomal replication is initiated from the replication origin oriC in Escherichia coli by the active ATP-bound form of DnaA protein. The regulatory inactivation of DnaA (RIDA) system, a complex of the ADP-bound Hda and the DNA-loaded replicase clamp, represses extra initiations by facilitating DnaA-bound ATP hydrolysis, yielding the inactive ADP-bound form of DnaA. However, the mechanisms involved in promoting the DnaA-Hda interaction have not been determined except for the involvement of an interaction between the AAA+ domains of the two. This study revealed that DnaA Leu-422 and Pro-423 residues within DnaA domain IV, including a typical DNA-binding HTH motif, are specifically required for RIDA-dependent ATP hydrolysis in vitro and that these residues support efficient interaction with the DNA-loaded clamp·Hda complex and with Hda in vitro. Consistently, substitutions of these residues caused accumulation of ATP-bound DnaA in vivo and oriC-dependent inhibition of cell growth. Leu-422 plays a more important role in these activities than Pro-423. By contrast, neither of these residues is crucial for DNA replication from oriC, although they are highly conserved in DnaA orthologues. Structural analysis of a DnaA·Hda complex model suggested that these residues make contact with residues in the vicinity of the Hda AAA+ sensor I that participates in formation of a nucleotide-interacting surface. Together, the results show that functional DnaA-Hda interactions require a second interaction site within DnaA domain IV in addition to the AAA+ domain and suggest that these interactions are crucial for the formation of RIDA complexes that are active for DnaA-ATP hydrolysis.

  5. DnaA Protein DNA-binding Domain Binds to Hda Protein to Promote Inter-AAA+ Domain Interaction Involved in Regulatory Inactivation of DnaA*

    Science.gov (United States)

    Keyamura, Kenji; Katayama, Tsutomu

    2011-01-01

    Chromosomal replication is initiated from the replication origin oriC in Escherichia coli by the active ATP-bound form of DnaA protein. The regulatory inactivation of DnaA (RIDA) system, a complex of the ADP-bound Hda and the DNA-loaded replicase clamp, represses extra initiations by facilitating DnaA-bound ATP hydrolysis, yielding the inactive ADP-bound form of DnaA. However, the mechanisms involved in promoting the DnaA-Hda interaction have not been determined except for the involvement of an interaction between the AAA+ domains of the two. This study revealed that DnaA Leu-422 and Pro-423 residues within DnaA domain IV, including a typical DNA-binding HTH motif, are specifically required for RIDA-dependent ATP hydrolysis in vitro and that these residues support efficient interaction with the DNA-loaded clamp·Hda complex and with Hda in vitro. Consistently, substitutions of these residues caused accumulation of ATP-bound DnaA in vivo and oriC-dependent inhibition of cell growth. Leu-422 plays a more important role in these activities than Pro-423. By contrast, neither of these residues is crucial for DNA replication from oriC, although they are highly conserved in DnaA orthologues. Structural analysis of a DnaA·Hda complex model suggested that these residues make contact with residues in the vicinity of the Hda AAA+ sensor I that participates in formation of a nucleotide-interacting surface. Together, the results show that functional DnaA-Hda interactions require a second interaction site within DnaA domain IV in addition to the AAA+ domain and suggest that these interactions are crucial for the formation of RIDA complexes that are active for DnaA-ATP hydrolysis. PMID:21708944

  6. Proton decay: Numerical simulations confront grand unification

    International Nuclear Information System (INIS)

    Brower, R.C.; Maturana, G.; Giles, R.C.; Moriarty, K.J.M.; Samuel, S.

    1985-01-01

    The Grand Unified Theories of the electromagnetic, weak and strong interactions constitute a far reaching attempt to synthesize our knowledge of theoretical particle physics into a consistent and compelling whole. Unfortunately, many quantitative predictions of such unified theories are sensitive to the analytically intractible effects of the strong subnuclear theory (Quantum Chromodynamics or QCD). The consequence is that even ambitious experimental programs exploring weak and super-weak interaction effects often fail to give definitive theoretical tests. This paper describes large-scale calculations on a supercomputer which can help to overcome this gap between theoretical predictions and experimental results. Our focus here is on proton decay, though the methods described are useful for many weak processes. The basic algorithms for the numerical simulation of QCD are well known. We will discuss the advantages and challenges of applying these methods to weak transitions. The algorithms require a very large data base with regular data flow and are natural candidates for vectorization. Also, 32-bit floating point arithmetic is adequate. Thus they are most naturally approached using a supercomputer alone or in combination with a dedicated special purpose processor. (orig.)

  7. Coordinating the Atlantis Grand Opening Event

    Science.gov (United States)

    Smith, Abigail Allaine

    2013-01-01

    While working as the marketing intern for Kennedy Space Center's (KSC) Center Planning and Development Directorate (CPD), I was given the opportunity not only to learn new skills and expand my work experience under the powerful influence of NASA, but I also was given the opportunity to prove that I am an individual capable of contributing to the KSC team. My main responsibility while working in CPD was to organize and run a booth that represented the entire directorate at the Grand Opening of the Shuttle Atlantis exhibit during the weekend of June 28-30. This event was important for CPD because as a Directorate that markets KSC's facilities, technical capabilities and technologies to potential partners, it is important to attend all events and use them to gain an understanding of our audience. Although we catered mostly to the general public during this event, it was still important to reach out to the larger space enthusiast community and let them know about KSC's future plans to become a multi-user spaceport and that teclmology is still being developed.

  8. The grand descent has begun for CMS

    CERN Multimedia

    2006-01-01

    Until recently, the CMS experimental cavern looked relatively empty; its detector was assembled entirely at ground level, to be lowered underground in 15 sections. On 2 November, the first hadronic forward calorimeter led the way with a grand descent. The first section of the CMS detector (centre of photo) arriving from the vertical shaft, viewed from the cavern floor. There is something unusual about the construction of the CMS detector. Instead of being built in the experimental cavern, like all the other detectors in the LHC experiments, it was constructed at ground level. This was to allow for easy access during the assembly of the detector and to minimise the size of the excavated cavern. The slightly nerve-wracking task of lowering it safely into the cavern in separate sections came after the complete detector was successfully tested with a magnetic field at ground level. In the early morning of 2 November, the first section of the CMS detector began its eagerly awaited descent into the underground ca...

  9. Local grand unification in the heterotic landscape

    International Nuclear Information System (INIS)

    Schmidt, Jonas

    2009-06-01

    We consider the possibility that the unification of the electroweak interactions and the strong force arises from string theory, at energies significantly lower than the string scale. As a tool, an effective grand unified field theory in six dimensions is derived from an anisotropic orbifold compactification of the heterotic string. It is explicitly shown that all anomalies cancel in the model, though anomalous Abelian gauge symmetries are present locally at the boundary singularities. In the supersymmetric vacuum additional interactions arise from higher-dimensional operators. We develop methods that relate the couplings of the effective theory to the location of the vacuum, and find that unbroken discrete symmetries play an important role for the phenomenology of orbifold models. An efficient algorithm for the calculation of the superpotential to arbitrary order is developed, based on symmetry arguments. We furthermore present a correspondence between bulk fields of the orbifold model in six dimensions, and the moduli fields that arise from compactifying four internal dimensions on a manifold with non-trivial gauge background. (orig.)

  10. Gauge hierarchy problem in grand unified theories

    International Nuclear Information System (INIS)

    Alhendi, H.A.A.

    1982-01-01

    In grand unification schemes, several mass scales are to be introduced, with some of them much larger than all the others, to cope with experimental observations, in which elementary particles of higher masses require higher energy to observe them than elementary particles of lower masses. There have been controversial arguments in the literature on such hierarchical scale structure, when radiative corrections are taken into account. It has been asserted that the gauge hierarchy depends on the choice of the subtraction point (in the classical field space), of the four-point function at zero external momentum. It also has been asserted that the gauge hierarchy problem whenever it is possible to be maintained in one sector of particles, it also is possible to be maintained in the other sectors. These two problems have been studied in a prototype model, namely an 0(3)-model with two triplets of real scalar Higgs fields. Our analysis shows that, within ordinary perturbation theory, none of these two problems is quite correct

  11. Integrated telemedicine workstation for intercontinental grand rounds

    Science.gov (United States)

    Willis, Charles E.; Leckie, Robert G.; Brink, Linda; Goeringer, Fred

    1995-04-01

    The Telemedicine Spacebridge to Moscow was a series of intercontinental sessions sponsored jointly by NASA and the Moscow Academy of Medicine. To improve the quality of medical images presented, the MDIS Project developed a workstation for acquisition, storage, and interactive display of radiology and pathology images. The workstation was based on a Macintosh IIfx platform with a laser digitizer for radiographs and video capture capability for microscope images. Images were transmitted via the Russian Lyoutch Satellite which had only a single video channel available and no high speed data channels. Two workstations were configured -- one for use at the Uniformed Services University of Health Sciences in Bethesda, MD. and the other for use at the Hospital of the Interior in Moscow, Russia. The two workstations were used may times during 16 sessions. As clinicians used the systems, we modified the original configuration to improve interactive use. This project demonstrated that numerous acquisition and output devices could be brought together in a single interactive workstation. The video images were satisfactory for remote consultation in a grand rounds format.

  12. Grand unification in the projective plane

    International Nuclear Information System (INIS)

    Hebecker, A.

    2004-01-01

    A 6-dimensional grand unified theory with the compact space having the topology of a real projective plane, i.e., a 2-sphere with opposite points identified, is considered. The space is locally flat except for two conical singularities where the curvature is concentrated. One supersymmetry is preserved in the effective 4d theory. The unified gauge symmetry, for example SU(5), is broken only by the non-trivial global topology. In contrast to the Hosotani mechanism, no adjoint Wilson-line modulus associated with this breaking appears. Since, locally, SU(5) remains a good symmetry everywhere, no UV-sensitive threshold corrections arise and SU(5)-violating local operators are forbidden. Doublet-triplet splitting can be addressed in the context of a 6d N=2 super Yang-Mills theory with gauge group SU(6). If this symmetry is first broken to SU(5) at a fixed point and then further reduced to the standard model group in the above non-local way, the two light Higgs doublets of the MSSM are predicted by the group-theoretical and geometrical structure of the model. (author)

  13. y las grandes ciudades: 1970-2004

    Directory of Open Access Journals (Sweden)

    Adrián de León Arias

    2008-01-01

    Full Text Available El cambio regional en las manufacturas mexicanas a partir de la década de 1980 se ha caracterizado por el rápido crecimiento del empleo y la producción en la frontera norte del país y la pérdida de participación de ellos en las grandes ciudades. En este artículo se analiza y evalúa este cambio regional en términos de la dinámica de la productividad laboral y factorial total para ambas configuraciones espaciales, presentando cálculos de productividad para éstas de 1970 a 2004, estimados a partir de la información censal. Los resultados sugieren que mientras el empleo ha crecido en magnitud mucho más rápido en la frontera norte, esta dinámica se ha generado con bajos niveles en crecimiento de la productividad, al menos hasta fechas recientes, por lo que el cambio regional estimulado por la apertura comercial hasta 2004 no parece haber facilitado un crecimiento económico más acelerado para México

  14. Local grand unification in the heterotic landscape

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, Jonas

    2009-07-15

    We consider the possibility that the unification of the electroweak interactions and the strong force arises from string theory, at energies significantly lower than the string scale. As a tool, an effective grand unified field theory in six dimensions is derived from an anisotropic orbifold compactification of the heterotic string. It is explicitly shown that all anomalies cancel in the model, though anomalous Abelian gauge symmetries are present locally at the boundary singularities. In the supersymmetric vacuum additional interactions arise from higher-dimensional operators. We develop methods that relate the couplings of the effective theory to the location of the vacuum, and find that unbroken discrete symmetries play an important role for the phenomenology of orbifold models. An efficient algorithm for the calculation of the superpotential to arbitrary order is developed, based on symmetry arguments. We furthermore present a correspondence between bulk fields of the orbifold model in six dimensions, and the moduli fields that arise from compactifying four internal dimensions on a manifold with non-trivial gauge background. (orig.)

  15. Constraints on grand unified superstring theories

    International Nuclear Information System (INIS)

    Ellis, J.; Lopez, J.L.; Nanopoulos, D.V.; Houston Advanced Research Center

    1990-01-01

    We evaluate some constraints on the construction of grand unified superstring theories (GUSTs) using higher level Kac-Moody algebras on the world-sheet. In the most general formulation of the heterotic string in four dimensions, an analysis of the basic GUST model-building constraints, including a realistic hidden gauge group, reveals that there are no E 6 models and any SO(10) models can only exist at level-5. Also, any such SU(5) models can exist only for levels 4≤k≤19. These SO(10) and SU(5) models risk having many large, massless, phenomenologically troublesome representations. We also show that with a suitable hidden sector gauge group, it is possible to avoid free light fractionally charged particles, which are endemic to string derived models. We list all such groups and their representations for the flipped SU(5)xU(1) model. We conclude that a sufficiently binding hidden sector gauge group becomes a basic model-building constraint. (orig.)

  16. A unified grand tour of theoretical physics

    CERN Document Server

    Griffiths, J

    2002-01-01

    Anyone offering a grand tour is faced with several options. Should they concentrate on what may be considered to be essential features, or should they attempt to present a brief glimpse of almost everything? The present offering is a compromise between these two extremes. The area considered - theoretical physics - is now such a vast subject that some kind of compromise is essential. Indeed, the field is now so wide that few could even attempt to review it in a single-authored work. My task here is to assess how well this book has succeeded in its main aim of providing a unified (though introductory) tour of this subject. Constrained within a single volume, this is clearly not an updated Landau-Lifschitz. It cannot be expected to take any particular topic to the level of recent research. Nevertheless, it does seem to cover the broad range of essential topics which now constitute the subject. It starts (most appropriately in my opinion) with geometry. It then covers classical physics, general relativity and qu...

  17. Dosimetric accuracy and clinical quality of Acuros XB and AAA dose calculation algorithm for stereotactic and conventional lung volumetric modulated arc therapy plans

    International Nuclear Information System (INIS)

    Kroon, Petra S; Hol, Sandra; Essers, Marion

    2013-01-01

    The main aim of the current study was to assess the dosimetric accuracy and clinical quality of volumetric modulated arc therapy (VMAT) plans for stereotactic (stage I) and conventional (stage III) lung cancer treatments planned with Eclipse version 10.0 Anisotropic Analytical Algorithm (AAA) and Acuros XB (AXB) algorithm. The dosimetric impact of using AAA instead of AXB, and grid size 2.5 mm instead of 1.0 mm for VMAT treatment plans was evaluated. The clinical plan quality of AXB VMAT was assessed using 45 stage I and 73 stage III patients, and was compared with published results, planned with VMAT and hybrid-VMAT techniques. The dosimetric impact on near-minimum PTV dose (D 98% ) using AAA instead of AXB was large (underdose up to 12.3%) for stage I and very small (underdose up to 0.8%) for stage III lung treatments. There were no significant differences for dose volume histogram (DVH) values between grid sizes. The calculation time was significantly higher for AXB grid size 1.0 than 2.5 mm (p < 0.01). The clinical quality of the VMAT plans was at least comparable with clinical qualities given in literature of lung treatment plans with VMAT and hybrid-VMAT techniques. The average mean lung dose (MLD), lung V 20Gy and V 5Gy in this study were respectively 3.6 Gy, 4.1% and 15.7% for 45 stage I patients and 12.4 Gy, 19.3% and 46.6% for 73 stage III lung patients. The average contra-lateral lung dose V 5Gy-cont was 35.6% for stage III patients. For stereotactic and conventional lung treatments, VMAT calculated with AXB grid size 2.5 mm resulted in accurate dose calculations. No hybrid technique was needed to obtain the dose constraints. AXB is recommended instead of AAA for avoiding serious overestimation of the minimum target doses compared to the actual delivered dose

  18. MODELADO DE LAS ISOTERMAS DE SORCIÓN Y DEL CALOR ISOSTÉRICO DE SORCIÓN EN PULPA DE MANGO cv. TOMMY ATKINS MODELAGEM DE ISOTERMAS DE SORÇÃO E CALOR ISOSTÉRICO DE SORÇÃO DE POLPA DE MANGA cv. TOMMY ATKINS MODELING SORPTION ISOTHERMS AND ISOSTERIC HEAT OF SORPTION OF MANGO PULP cv. TOMMY ATKINS

    Directory of Open Access Journals (Sweden)

    JOSÉ BON

    2012-12-01

    Full Text Available El mango es un producto agrícola de gran importancia para diferentes países en vías de desarrollo, el cual ha incrementado su demanda por sus propiedades nutricionales y características exóticas. La relación entre el contenido de humedad y la actividad de agua suministra información útil para el procesamiento del mango, especialmente en las operaciones de secado y almacenamiento. La actividad de agua y el contenido de humedad de la pulpa de mango (Mangifera indica L. cv. Tommy Atkins fue analizada para determinar las isotermas de sorción. La actividad de agua fue medida utilizando un higrómetro eléctrico a cinco diferentes temperaturas (10, 20, 30, 40 y 50 °C y amplios rangos de contenido de humedad (2.829-0.031 kg kg-1 b.s. y actividad de agua (0.977-0.215. Un modelo teórico (GAB y cuatro ecuaciones empíricas (Oswin, Henderson, Halsey y Ratti fueron utilizados para representar las isotermas de sorción. Después de considerar diferentes criterios, el modelo de GAB fue evaluado como la mejor opción para representar el equilibrio higroscópico de la pulpa de mango (RMSE = 0.057, VAR = 0.996. Las isotermas mostraron un comportamiento acorde a los materiales agroalimentarios y similar al reportado para pulpa de mango de otras variedades. El calor isostérico de sorción fue calculado utilizando la ecuación de Clausius-Clapeyron a partir del modelo de GAB. Se observó una disminución en el calor isostérico de sorción al aumentar el contenido de humedad de equilibrio, con gran efecto de la humedad a valores por debajo de 0.8 kg kg-1 (b.s..A manga é um produto agrícola em países de desenvolvimento econômico, com grande aceitação devido a suas propriedades nutricionais e características exóticas. A relação entre o conteúdo de umidade e atividade de água proporciona informação útil para o processamento do mango, especialmente nas operações de secagem e armazenamento. A atividade de água e o conteúdo de umidade da

  19. Agrobacterium-mediated transformation of Easter lily (Lilium longiflorum cv. Nellie White)

    Science.gov (United States)

    Conditions were optimized for transient transformation of Lilium longiflorum cv. Nellie White using Agrobacterium tumefaciens. Bulb scale and basal meristem explants were inoculated with A. tumefaciens strain AGL1 containing the binary vector pCAMBIA 2301 which has the uidA gene that codes for ß-gl...

  20. Possibility of Recombination Gain Increase in CV Ions at 4.0 nm Via Coherence

    Science.gov (United States)

    Luo, Y.; Morozov, A.; Gordon, D.; Sprangle, P.; Svidzinsky, A.; Xia, H.; Scully, M.; Suckewer, S.

    This paper is about the recent experimental results on amplification of the CV line in the "water window" at 4.03 nm from resonance transition to the ground level of He-like ions in recombination scheme. The indication of the amplification of the CV line has been observed when an elongated narrow plasma channel was created, where high intensity 100 fs beams, optimal for creating CV ions in high density plasma, was propagated up to 0.5-0.6 mm. Without channeling the effective plasma length was much shorter and there was no indication of amplification.The large interest in gain generation in He-like ions in the transition to ground state is due to the possibility of applying a recently developed theory of Lasing Without Inversion (LWI) in XUV and X-ray regions to largely increase the gain for such transitions. The presented results of the indication of CV line amplifications are being discussed from the point of view of using LWI as a superradiance gain increase, hence to construct a very compact soft X-ray laser in the "water window".The last part of the paper is related to the application of the ultra-intensive fs plasma laser, which is currently in the process of development by using stimulated Raman backscattering (SRBS) to create a plasma amplifier and compressor, as the pump for compact laser operating in the "water window" and also at shorter wavelengths.

  1. RADISH CV. MOKHOVSKIY IS A SOURCE OF MS- AND MF-LINES AT BREEDING FOR HETEROSIS

    Directory of Open Access Journals (Sweden)

    M. I. Fedorova

    2015-01-01

    Full Text Available Based on hybrid and inbreed population of radish cv. Mokhovskiy using CMS-ogura nine lines with 100% of male sterility and fertile maintainers were selected. Using the multiplex PCR, the presence of cytoplasm of Ogura type in ms-lines and its absence in mf-lines were confirmed.

  2. Limited geographic distribution of the novel cyclovirus CyCV-VN

    NARCIS (Netherlands)

    Le, Van Tan; de Jong, Menno D.; Nguyen, Van Kinh; Nguyen, Vu Trung; Taylor, Walter; Wertheim, Heiman F. L.; van der Ende, Arie; van der Hoek, Lia; Canuti, Marta; Crusat, Martin; Sona, Soeng; Nguyen, Hanh Uyen; Giri, Abhishek; Nguyen, Thi Thuy Chinh Bkrong; Ho, Dang Trung Nghia; Farrar, Jeremy; Bryant, Juliet E.; Tran, Tinh Hien; Nguyen, Van Vinh Chau; van Doorn, H. Rogier

    2014-01-01

    A novel cyclovirus, CyCV-VN, was recently identified in cerebrospinal fluid (CSF) from patients with central nervous system (CNS) infections in central and southern Vietnam. To explore the geographic distribution of this novel virus, more than 600 CSF specimens from patients with suspected CNS

  3. Automatic tracking of red blood cells in micro channels using OpenCV

    Science.gov (United States)

    Rodrigues, Vânia; Rodrigues, Pedro J.; Pereira, Ana I.; Lima, Rui

    2013-10-01

    The present study aims to developan automatic method able to track red blood cells (RBCs) trajectories flowing through a microchannel using the Open Source Computer Vision (OpenCV). The developed method is based on optical flux calculation assisted by the maximization of the template-matching product. The experimental results show a good functional performance of this method.

  4. Cherry Picking Robot Vision Recognition System Based on OpenCV

    Directory of Open Access Journals (Sweden)

    Zhang Qi Rong

    2016-01-01

    Full Text Available Through OpenCV function, the cherry in a natural environment image after image preprocessing, color recognition, threshold segmentation, morphological filtering, edge detection, circle Hough transform, you can draw the cherry’s center and circular contour, to carry out the purpose of the machine picking. The system is simple and effective.

  5. Production of calibration sources and/or radioactive tracers with the cyclotron CV-28

    International Nuclear Information System (INIS)

    Osso Junior, Joao Alberto

    1995-01-01

    The present stage of production of calibration sources and radioactive tracers with the Cyclotron CV-28 is described. Among the methods already developed special attention is given to the production of 57 Co, 109 Cd and 111 In. (author). 3 refs

  6. Lipid abnormalities in streptozotocin-diabetes: Amelioration by Morus indica L. cv Suguna leaves

    OpenAIRE

    Andallu, B.; Vinay Kumar, A. V.; Varadacharyulu, N. Ch.

    2009-01-01

    AIM: To observe the influence of mulberry (Morus indica L. cv Suguna) leaves on lipid abnormalities in STZ-diabetic rats. MATERIALS AND METHODS: Treatment with dried mulberry leaf powder for a period of 8 weeks in hyperglycemic and hyperlipidemic STZ-diabetic rats. RESULTS: Mulberry leaves regulated fasting blood glucose, ameliorated the abnormalities in lipid profile as indicated by significant (P

  7. Connected Vehicle Pilot Deployment Program, Comprehensive Installation Plan - WYDOT CV Pilot

    Science.gov (United States)

    2018-02-16

    The Wyoming Department of Transportation's (WYDOT) Connected Vehicle (CV) Pilot Deployment Program is intended to develop a suite of applications that utilize vehicle-to-infrastructure (V2I) and vehicle-to-vehicle (V2V) communication technology to re...

  8. NALISIS PENGAKUAN PENDAPATAN BERDASARKAN PSAK NOMOR 23 PADA CV TILZAK KABUPATEN PINRANG

    Directory of Open Access Journals (Sweden)

    ILHAM ARIFIN

    2017-05-01

    This research was conducted at CV. Tilzak Pinrang which is a business as a cog in the business of selling. Based on research that has been done, it is not all revenue recognition in accordance with IAS 23 is not dibuatkannya marked revenue balance sheet and income statement, so that the impact to the company's earnings were only included proceeds earned in one year.

  9. STRATEGI SEGMENTASI PASAR TERHADAP VOLUME PENJUALAN BUSANA MUSLIM PADA CV RABBANI ASYSA MAKASSAR

    OpenAIRE

    KARASE, HASNAWATI

    2014-01-01

    2014 Hasnawati Karase Haris Maupa Mukhtar Penelitian ini bertujuan untuk menganalisis strategi segmentasi pasar terhadap volume penjualan busana muslim pada CV Rabbani Asysa Makassar. Data penelitian ini diperoleh dari kuesioner (primer) mengenai segmentasi pasar Rabbani Makassar dengan mengambil sampel sebanyak 75 responden. Temuan penelitian ini menunjukkan bahwa variabel strategi segmentasi pasar yang terdiri dari segmentasi geografis, segmentasi demografis, segm...

  10. PlantCV v2: Image analysis software for high-throughput plant phenotyping

    Directory of Open Access Journals (Sweden)

    Malia A. Gehan

    2017-12-01

    Full Text Available Systems for collecting image data in conjunction with computer vision techniques are a powerful tool for increasing the temporal resolution at which plant phenotypes can be measured non-destructively. Computational tools that are flexible and extendable are needed to address the diversity of plant phenotyping problems. We previously described the Plant Computer Vision (PlantCV software package, which is an image processing toolkit for plant phenotyping analysis. The goal of the PlantCV project is to develop a set of modular, reusable, and repurposable tools for plant image analysis that are open-source and community-developed. Here we present the details and rationale for major developments in the second major release of PlantCV. In addition to overall improvements in the organization of the PlantCV project, new functionality includes a set of new image processing and normalization tools, support for analyzing images that include multiple plants, leaf segmentation, landmark identification tools for morphometrics, and modules for machine learning.

  11. The influence of virus diseases on grape polyphenols of cv. 'Refosk'

    International Nuclear Information System (INIS)

    Tomazic, I.; Vrhovsek, U.; Korosec-Koruza, Z.

    2003-01-01

    External stimuli such as microbial infections, ultraviolet radiation, and chemical stressors can modulate the synthesis of polyphenols in the plants. Cv. 'Refosk' was used to show the influence of the GLRaV-1 and rugose wood (RW) on the polyphenols in grape. The infection shifted polyphenols from seeds to grape skins but had no impact on anthocyanins

  12. Endogenous auxin regulates the sensitivity of Dendrobium (cv. Miss Teen) flower pedicel abscission to ethylene

    NARCIS (Netherlands)

    Rungruchkanont, K.; Ketsa, S.; Chatchawankanphanich, O.; Doorn, van W.G.

    2007-01-01

    Dendrobium flower buds and flowers have an abscission zone at the base of the pedicel (flower stalk). Ethylene treatment of cv. Miss Teen inflorescences induced high rates of abscission in flower buds but did not affect abscission once the flowers had opened. It is not known if auxin is a regulator

  13. ANALISIS SEWA MENYEWA PARALEL PADA PERUSAHAAN RENT CAR CV. HARKAT DALAM PERSPEKTIF EKONOMI ISLAM

    Directory of Open Access Journals (Sweden)

    Samsuardi Samsuardi

    2013-12-01

    Full Text Available This study aims at determining the parallel process of car rental agreement between Harkat Rent Car Company and the owner of the car, and between the company and its customers in an Islamic economics perspective. This study also aims to analyze the risk coverage of the leased car. The data for this study was gathered through interviews, observation and documentation studies. The collected data was then analyzed using descriptive analysis method. Results showed that the process of car leasing begin when customer rented a car from CV. Harkat which was owned by its partner. The company provided a shared price for car owners according to a mutual agreement and applied only for a certain period. Furthermore, the partner has to allocate 20 percent of the income for the company. In addition, the company also required the partner to cover for maintenance costs, insurance, equipment, and spare parts. In the context of Islamic economics, the practice of leasing parallel conducted by the company was not fully Shariah compliance due to practice a lease above a lease and using two contracts in one transaction. =========================================== Penelitian ini bertujuan untuk mengetahui perjanjian sewa menyewa mobil secara paralel antara Perusahaan Rent Car CV. Harkat dengan pemilik mobil dan dengan konsumennya dalam perspektif ekonomi Islam. Penelitian ini juga bertujuan untuk menanganalisis pertanggungan resiko terhadap mobil yang dijadikan objek sewa menyewa tersebut sewa menyewa mobil di Perusahaan Rent Car CV. Data untuk penelitian ini bersumber dari wawancara, obesrvasi dan studi dokumentasi. Metode yang digunakan dalam penelitian ini adalah deskriptif analisis. Hasil penelitian menunjukkan bahwa CV. Harkat menyewakan mobil milik mitra kerjanya (pemilik mobil kepada pelanggan yang membutuhkan sewa mobil untuk keperluannya. Pemilik usaha memberikan harga kongsi kepada pemilik mobil sesuai dengan kesepakatan bersama dan untuk jangka waktu

  14. AAA+ ATPases Reptin and Pontin as potential diagnostic and prognostic biomarkers in salivary gland cancer - a short report.

    Science.gov (United States)

    Mikesch, Jan-Henrik; Hartmann, Wolfgang; Angenendt, Linus; Huber, Otmar; Schliemann, Christoph; Arteaga, Maria Francisca; Wardelmann, Eva; Rudack, Claudia; Berdel, Wolfgang E; Stenner, Markus; Grünewald, Inga

    2018-06-05

    Salivary gland cancer (SGC) is a rare and heterogeneous disease with significant differences in recurrence and metastasis characteristics. As yet, little is known about the mechanisms underlying the initiation and/or progression of these diverse tumors. In recent years, the AAA+ ATPase family members Pontin (RuvBL1, Tip49a) and Reptin (RuvBL2, Tip49b) have been implicated in various processes, including transcription regulation, chromatin remodeling and DNA damage repair, that are frequently deregulated in cancer. The aim of this study was to assess the clinical and functional significance of Reptin and Pontin expression in SGC. Immunohistochemical staining of Pontin, Reptin, β-catenin, Cyclin D1, TP53 and MIB-1 was performed on a collection of 94 SGC tumor samples comprising 13 different histological subtypes using tissue microarrays. We found that Reptin and Pontin were expressed in the majority of SGC samples across all histological subtypes. Patients with a high Reptin expression showed a significantly inferior 5-year overall survival rate compared to patients with a low Reptin expression (47.7% versus 78.3%; p = 0.033), whereas no such difference was observed for Pontin. A high Reptin expression strongly correlated with a high expression of the proliferation marker MIB-1 (p = 0.003), the cell cycle regulator Cyclin D1 (p = 0.006), accumulation of TP53 as a surrogate p53 mutation marker (p = 0.042) and cytoplasmic β-catenin expression (p = 0.002). Increased Pontin expression was found to significantly correlate with both cytoplasmic and nuclear β-catenin expression (p = 0.037 and p = 0.018, respectively), which is indicative for its oncogenic function. Our results suggest a role of Reptin and Pontin in SGC tumor progression and/or patient survival. Therefore, SGC patients exhibiting a high Reptin expression may benefit from more aggressive therapeutic regimens. Future studies should clarify whether such patients may be considered

  15. TENCompetence Learning Design Toolkit, Runtime component, ccsi_v3_2_10c_v1_4

    NARCIS (Netherlands)

    Sharples, Paul; Popat, Kris; Llobet, Lau; Santos, Patricia; Hernández-Leo, Davinia; Miao, Yongwu; Griffiths, David; Beauvoir, Phillip

    2010-01-01

    Sharples, P., Popat, K., Llobet, L., Santos, P., Hernandez-Leo, D., Miao, Y., Griffiths, D. & Beauvoir, P. (2009) TENCompetence Learning Design Toolkit, Runtime component, ccsi_v3_2_10c_v1_4 This release is composed of three files corresponding to CopperCore Service Integration (CCSI) v3.2-10cv1.4,

  16. VT Built Up Lands in Grand Isle County - 1986

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  17. VT Built Up Lands in Grand Isle County - 1941

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  18. Constructing 5d orbifold grand unified theories from heterotic strings

    International Nuclear Information System (INIS)

    Kobayashi, Tatsuo; Raby, Stuart; Zhang Renjie

    2004-01-01

    A three-generation Pati-Salam model is constructed by compactifying the heterotic string on a particular T 6 /Z 6 Abelian symmetric orbifold with two discrete Wilson lines. The compactified space is taken to be the Lie algebra lattice G 2 -bar SU(3)-bar SO(4). When one dimension of the SO(4) lattice is large compared to the string scale, this model reproduces many features of a 5d SO(10) grand unified theory compactified on an S 1 /Z 2 orbifold. (Of course, with two large extra dimensions we can obtain a 6d SO(10) grand unified theory.) We identify the orbifold parities and other ingredients of the orbifold grand unified theories in the string model. Our construction provides a UV completion of orbifold grand unified theories, and gives new insights into both field theoretical and string theoretical constructions

  19. 27 CFR 9.119 - Middle Rio Grande Valley.

    Science.gov (United States)

    2010-04-01

    ... Middle Rio Grande Valley. (a) Name. The name of the viticultural area described in this section is... 1979. (24) Veguita, N. Mex. (1952), revised 1979. (25) Wind Mesa, N. Mex. (1952), revised 1967. (c...

  20. A GRAND PLAN FOR EARTH LOVE EDUCATION IN SOUTHERN ...

    African Journals Online (AJOL)

    cognition. From this doubtful synthesis, the researcher appears to have constructed a grand theory of how the world without ... problem here is that all of the students (English. Xhosa and ..... accompanying 'earthy music' will contrihute to.