Lamin A reassembly at the end of mitosis is regulated by its SUMO-interacting motif

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Moriuchi, Takanobu; Kuroda, Masaki; Kusumoto, Fumiya; Osumi, Takashi; Hirose, Fumiko, E-mail: fhirose@sci.u-hyogo.ac.jp

2016-03-01

Modification of proteins with small ubiquitin-related modifier (SUMO; SUMOylation) is involved in the regulation of various biological processes. Recent studies have demonstrated that noncovalent associations between SUMOylated proteins and co-operative proteins containing SUMO-interacting motifs (SIMs) are important for the spatiotemporal organization of many protein complexes. In this study, we demonstrate that interactions between lamin A, a major component of the nuclear lamina, and SUMO isoforms are dependent on one of the four SIMs (SIM3) resided in lamin A polypeptide in vitro. Live cell imaging and immunofluorescence staining showed that SIM3 is required for accumulation of lamin A on the chromosomes during telophase, and subsequent evaluation of a panel of deletion mutants determined that a 156-amino acid region spanning the carboxyl-terminal Ig-fold domain of lamin A is sufficient for this accumulation. Notably, mutation of SIM3 abrogated the dephosphorylation of mitosis-specific phosphorylation at Ser-22 of lamin A, which normally occurs during telophase, and the subsequent nuclear lamina reorganization. Furthermore, expression of a conjugation-defective SUMO2 mutant, which was previously shown to inhibit endogenous SUMOylation in a dominant-negative manner, also impaired the accumulation of wild type lamin A on telophase chromosomes. These findings suggest that interactions between SIM3 of lamin A and a putative SUMO2-modified protein plays an important role in the reorganization of the nuclear lamina at the end of mitosis. - Highlights: • Lamin A interacts with SUMO2 via a SUMO-interacting motif (SIM) in the Ig domain. • SIM3 of lamin A is responsible for chromosomal accumulation during telophase. • A 156-aa region spanning the Ig domain is sufficient for chromosomal accumulation. • Accumulation of lamin A is required for timely dephosphorylation on chromosomes. • A putative SUMO2-modified protein may mediate chromosomal accumulation of lamin

Lamin A reassembly at the end of mitosis is regulated by its SUMO-interacting motif

International Nuclear Information System (INIS)

Moriuchi, Takanobu; Kuroda, Masaki; Kusumoto, Fumiya; Osumi, Takashi; Hirose, Fumiko

2016-01-01

Modification of proteins with small ubiquitin-related modifier (SUMO; SUMOylation) is involved in the regulation of various biological processes. Recent studies have demonstrated that noncovalent associations between SUMOylated proteins and co-operative proteins containing SUMO-interacting motifs (SIMs) are important for the spatiotemporal organization of many protein complexes. In this study, we demonstrate that interactions between lamin A, a major component of the nuclear lamina, and SUMO isoforms are dependent on one of the four SIMs (SIM3) resided in lamin A polypeptide in vitro. Live cell imaging and immunofluorescence staining showed that SIM3 is required for accumulation of lamin A on the chromosomes during telophase, and subsequent evaluation of a panel of deletion mutants determined that a 156-amino acid region spanning the carboxyl-terminal Ig-fold domain of lamin A is sufficient for this accumulation. Notably, mutation of SIM3 abrogated the dephosphorylation of mitosis-specific phosphorylation at Ser-22 of lamin A, which normally occurs during telophase, and the subsequent nuclear lamina reorganization. Furthermore, expression of a conjugation-defective SUMO2 mutant, which was previously shown to inhibit endogenous SUMOylation in a dominant-negative manner, also impaired the accumulation of wild type lamin A on telophase chromosomes. These findings suggest that interactions between SIM3 of lamin A and a putative SUMO2-modified protein plays an important role in the reorganization of the nuclear lamina at the end of mitosis. - Highlights: • Lamin A interacts with SUMO2 via a SUMO-interacting motif (SIM) in the Ig domain. • SIM3 of lamin A is responsible for chromosomal accumulation during telophase. • A 156-aa region spanning the Ig domain is sufficient for chromosomal accumulation. • Accumulation of lamin A is required for timely dephosphorylation on chromosomes. • A putative SUMO2-modified protein may mediate chromosomal accumulation of lamin

Structure of the lamin A/C R482W mutant responsible for dominant familial partial lipodystrophy (FPLD)

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Magracheva, Eugenia; Kozlov, Serguei; Stewart, Colin L.; Wlodawer, Alexander; Zdanov, Alexander; (NCI)

2009-08-07

Proteins of the A-type lamin family, which consists of two members, lamin A and lamin C, are the major components of a thin proteinaceous filamentous meshwork, the lamina, that underlies the inner nuclear membrane. A-type lamins have recently become the focus of extensive functional studies as a consequence of the linking of at least eight congenital diseases to mutations in the lamin A/C gene (LMNA). This spectrum of pathologies, which mostly manifest themselves as dominant traits, includes muscle dystrophies, dilated cardiomyopathies, the premature aging syndrome Hutchinson-Guilford progeria and familial partial lipodystrophy (FPLD). The crystal structure of the lamin A/C mutant R482W, a variant that causes FPLD, has been determined at 1.5 {angstrom} resolution. A completely novel aggregation state of the C-terminal globular domain and the position of the mutated amino-acid residue suggest means by which the mutation may affect lamin A/C-protein and protein-DNA interactions.

Mutation Glu82Lys in lamin A/C gene is associated with cardiomyopathy and conduction defect

International Nuclear Information System (INIS)

Wang Hu; Wang Jizheng; Zheng Weiyue; Wang Xiaojian; Wang Shuxia; Song Lei; Zou Yubao; Yao Yan; Hui Rutai

2006-01-01

Dilated cardiomyopathy is a form of heart muscle disease characterized by impaired systolic function and ventricular dilation. The mutations in lamin A/C gene have been linked to dilated cardiomyopathy. We screened genetic mutations in a large Chinese family of 50 members including members with dilated cardiomyopathy and found a Glu82Lys substitution mutation in the rod domain of the lamin A/C protein in eight family members, three of them have been diagnosed as dilated cardiomyopathy, one presented with heart dilation. The pathogenic mechanism of lamin A/C gene defect is poorly understood. Glu82Lys mutated lamin A/C and wild type protein was transfected into HEK293 cells. The mutated protein was not properly localized at the inner nuclear membrane and the emerin protein, which interacts with lamin A/C, was also aberrantly distributed. The nuclear membrane structure was disrupted and heterochromatin was aggregated aberrantly in the nucleus of the HEK293 cells stably transfected with mutated lamin A/C gene as determined by transmission electron microscopy

Chromatin and lamin A determine two different mechanical response regimes of the cell nucleus.

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Stephens, Andrew D; Banigan, Edward J; Adam, Stephen A; Goldman, Robert D; Marko, John F

2017-07-07

The cell nucleus must continually resist and respond to intercellular and intracellular mechanical forces to transduce mechanical signals and maintain proper genome organization and expression. Altered nuclear mechanics is associated with many human diseases, including heart disease, progeria, and cancer. Chromatin and nuclear envelope A-type lamin proteins are known to be key nuclear mechanical components perturbed in these diseases, but their distinct mechanical contributions are not known. Here we directly establish the separate roles of chromatin and lamin A/C and show that they determine two distinct mechanical regimes via micromanipulation of single isolated nuclei. Chromatin governs response to small extensions (<3 μm), and euchromatin/heterochromatin levels modulate the stiffness. In contrast, lamin A/C levels control nuclear strain stiffening at large extensions. These results can be understood through simulations of a polymeric shell and cross-linked polymer interior. Our results provide a framework for understanding the differential effects of chromatin and lamin A/C in cell nuclear mechanics and their alterations in disease. © 2017 Stephens et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

LAMINATES

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Gökay Nemli

2004-04-01

Full Text Available Wood based panel producers afford to present their products either in sized semi-finished form or as covered in general by the in additional investments realized. The fact that the laminated material has a certain market share as well as the increase in demand for furniture types finished in various profiles have put the laminated sheets which provide very comprehensive design facilities at the top place and caused such boards to spread over the market rather more quickly. In line with this development, great developments have also been recorded during recent years in laminate utilization in furniture factoring sector and fast steps taken towards a more rational working environment. In this study, laminates types and manufacturing technologies were investigated.

A theory of piezoelectric laminates

International Nuclear Information System (INIS)

Giangreco, E.

1997-01-01

A theory of piezoelectric laminates is rationally derived from the three-dimensional Voigt theory of piezoelectricity. The present theory is a generalization to piezoelectric laminates of the Reissner-Mindlin-type layer-wise theory of elastic laminates. Both a differential formulation and a variational formulation of the piezoelectric laminate problem are presented. The proposed theory is adopted in the analysis of simple problems, in order to verify its effectiveness. The results it provides turn out to be in good agreement with the results supplied by the Voigt theory of piezoelectricity

NuMA and nuclear lamins are cleaved during viral infection - inhibition of caspase activity prevents cleavage and rescues HeLa cells from measles virus-induced but not from rhinovirus 1B-induced cell death

International Nuclear Information System (INIS)

Taimen, Pekka; Berghaell, Heidi; Vainionpaeae, Raija; Kallajoki, Markku

2004-01-01

Nuclear matrix is a structural framework of important nuclear processes. We studied the effect of two different types of viral infections on nuclear matrix. HeLa cells were infected with human rhinovirus 1B (HRV 1B) or measles virus (MV), and Nuclear Mitotic Apparatus protein (NuMA) and lamins A/C and B were used as markers for internal nuclear matrix and peripheral nuclear lamina, respectively. We show that NuMA, lamins, and poly(ADP-ribose) polymerase-1 are cleaved during viral infection in a virus family-specific manner suggesting that these viruses activate different sets of proteases. Morphologically, NuMA was excluded from the condensed chromatin, lamins showed a folded distribution, and both proteins finally remained around the nuclear fragments. A general caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (z-VAD-FMK) prevented the nuclear disintegration and the cleavage of the proteins studied. Interestingly, z-VAD-FMK rescued MV-infected but not HRV 1B-infected cells from cell death. These results show for the first time that NuMA and lamins are specific target proteins during virus-induced programmed cell death

Decreased Bone Formation and Osteopenia in Lamin A/C-Deficient Mice

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Vidal, Christopher; McCorquodale, Thomas; Herrmann, Markus; Fatkin, Diane; Duque, Gustavo

2011-01-01

Age-related bone loss is associated with changes in bone cellularity with characteristically low levels of osteoblastogenesis. The mechanisms that explain these changes remain unclear. Although recent in vitro evidence has suggested a new role for proteins of the nuclear envelope in osteoblastogenesis, the role of these proteins in bone cells differentiation and bone metabolism in vivo remains unknown. In this study, we used the lamin A/C null (Lmna −/−) mice to identify the role of lamin A/C in bone turnover and bone structure in vivo. At three weeks of age, histological and micro computed tomography measurements of femurs in Lmna −/− mice revealed a significant decrease in bone mass and microarchitecture in Lmna −/− mice as compared with their wild type littermates. Furthermore, quantification of cell numbers after normalization with bone surface revealed a significant reduction in osteoblast and osteocyte numbers in Lmna −/− mice compared with their WT littermates. In addition, Lmna −/− mice have significantly lower osteoclast number, which show aberrant changes in their shape and size. Finally, mechanistic analysis demonstrated that absence of lamin A/C is associated with increase expression of MAN-1 a protein of the nuclear envelope closely regulated by lamin A/C, which also colocalizes with Runx2 thus affecting its capacity as osteogenic transcription factor. In summary, these data clearly indicate that the presence of lamin A/C is necessary for normal bone turnover in vivo and that absence of lamin A/C induces low bone turnover osteopenia resembling the cellular changes of age-related bone loss. PMID:21547077

Ubiquitin ligase RNF123 mediates degradation of heterochromatin protein 1α and β in lamin A/C knock-down cells.

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Pankaj Chaturvedi

Full Text Available The nuclear lamina is a key determinant of nuclear architecture, integrity and functionality in metazoan nuclei. Mutations in the human lamin A gene lead to highly debilitating genetic diseases termed as laminopathies. Expression of lamin A mutations or reduction in levels of endogenous A-type lamins leads to nuclear defects such as abnormal nuclear morphology and disorganization of heterochromatin. This is accompanied by increased proteasomal degradation of certain nuclear proteins such as emerin, nesprin-1α, retinoblastoma protein and heterochromatin protein 1 (HP1. However, the pathways of proteasomal degradation have not been well characterized.To investigate the mechanisms underlying the degradation of HP1 proteins upon lamin misexpression, we analyzed the effects of shRNA-mediated knock-down of lamins A and C in HeLa cells. Cells with reduced levels of expression of lamins A and C exhibited proteasomal degradation of HP1α and HP1β but not HP1γ. Since specific ubiquitin ligases are upregulated in lamin A/C knock-down cells, further studies were carried out with one of these ligases, RNF123, which has a putative HP1-binding motif. Ectopic expression of GFP-tagged RNF123 directly resulted in degradation of HP1α and HP1β. Mutational analysis showed that the canonical HP1-binding pentapeptide motif PXVXL in the N-terminus of RNF123 was required for binding to HP1 proteins and targeting them for degradation. The role of endogenous RNF123 in the degradation of HP1 isoforms was confirmed by RNF123 RNAi experiments. Furthermore, FRAP analysis suggested that HP1β was displaced from chromatin in laminopathic cells.Our data support a role for RNF123 ubiquitin ligase in the degradation of HP1α and HP1β upon lamin A/C knock-down. Hence lamin misexpression can cause degradation of mislocalized proteins involved in key nuclear processes by induction of specific components of the ubiquitin-proteasome system.

Lamin A/C might be involved in the EMT signalling pathway.

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Zuo, Lingkun; Zhao, Huanying; Yang, Ronghui; Wang, Liyong; Ma, Hui; Xu, Xiaoxue; Zhou, Ping; Kong, Lu

2018-07-15

We have previously reported a heterogeneous expression pattern of the nuclear membrane protein lamin A/C in low- and high-Gleason score (GS) prostate cancer (PC) tissues, and we have now found that this change is not associated with LMNA mutations. This expression pattern appears to be similar to the process of epithelial to mesenchymal transition (EMT) or to that of mesenchymal to epithelial transition (MET). The role of lamin A/C in EMT or MET in PC remains unclear. Therefore, we first investigated the expression levels of and the associations between lamin A/C and several common EMT markers, such as E-cadherin, N-cadherin, β-catenin, snail, slug and vimentin in PC tissues with different GS values and in different cell lines with varying invasion abilities. Our results suggest that lamin A/C might constitute a type of epithelial marker that better signifies EMT and MET in PC tissue, since a decrease in lamin A/C expression in GS 4 + 5 cases is likely associated with the EMT process, while the re-expression of lamin A/C in GS 5 + 4 cases is likely linked with MET. The detailed GS better exhibited the changes in lamin A/C and the EMT markers examined. Lamin A/C overexpression or knockdown had an impact on EMT biomarkers in a cell model by direct regulation of β-catenin. Hence, we suggest that lamin A/C might serve as a reliable epithelial biomarker for the distinction of PC cell differentiation and might also be a fundamental factor in the occurrence of EMT or MET in PC. Copyright © 2018. Published by Elsevier B.V.

Werner complex deficiency in cells disrupts the Nuclear Pore Complex and the distribution of lamin B1.

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Li, Zhi; Zhu, Yizhou; Zhai, Yujia; R Castroagudin, Michelle; Bao, Yifei; White, Tommy E; Glavy, Joseph S

2013-12-01

From the surrounding shell to the inner machinery, nuclear proteins provide the functional plasticity of the nucleus. This study highlights the nuclear association of Pore membrane (POM) protein NDC1 and Werner protein (WRN), a RecQ helicase responsible for the DNA instability progeria disorder, Werner Syndrome. In our previous publication, we connected the DNA damage sensor Werner's Helicase Interacting Protein (WHIP), a binding partner of WRN, to the NPC. Here, we confirm the association of the WRN/WHIP complex and NDC1. In established WRN/WHIP knockout cell lines, we further demonstrate the interdependence of WRN/WHIP and Nucleoporins (Nups). These changes do not completely abrogate the barrier of the Nuclear Envelope (NE) but do affect the distribution of FG Nups and the RAN gradient, which are necessary for nuclear transport. Evidence from WRN/WHIP knockout cell lines demonstrates changes in the processing and nucleolar localization of lamin B1. The appearance of "RAN holes" void of RAN corresponds to regions within the nucleolus filled with condensed pools of lamin B1. From WRN/WHIP knockout cell line extracts, we found three forms of lamin B1 that correspond to mature holoprotein and two potential post-translationally modified forms of the protein. Upon treatment with topoisomerase inhibitors lamin B1 cleavage occurs only in WRN/WHIP knockout cells. Our data suggest the link of the NDC1 and WRN as one facet of the network between the nuclear periphery and genome stability. Loss of WRN complex leads to multiple alterations at the NPC and the nucleolus. © 2013. Published by Elsevier B.V. All rights reserved.

Isoprenylation is required for the processing of the lamin A precursor

International Nuclear Information System (INIS)

Beck, L.A.; Hosick, T.J.; Sinensky, M.

1990-01-01

The nuclear lamina proteins, prelamin A, lamin B, and a 70-kD lamina-associated protein, are posttranslationally modified by a metabolite derived from mevalonate. This modification can be inhibited by treatment with (3-R,S)-3-fluoromevalonate, demonstrating that it is isoprenoid in nature. We have examined the association between isoprenoid metabolism and processing of the lamin A precursor in human and hamster cells. Inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase by mevinolin (lovastatin) specifically depletes endogenous isoprenoid pools and inhibits the conversion of prelamin A to lamin A. Prelamin A processing is also blocked by mevalonate starvation of Mev-1, a CHO cell line auxotrophic for mevalonate. Moreover, inhibition of prelamin A processing by mevinolin treatment is rapidly reversed by the addition of exogenous mevalonate. Processing of prelamin A is, therefore, dependent on isoprenoid metabolism. Analysis of the conversion of prelamin A to lamin A by two independent methods, immunoprecipitation and two-dimensional nonequilibrium pH gel electrophoresis, demonstrates that a precursor-product relationship exists between prelamin A and lamin A. Analysis of R,S-[5-3H(N)]mevalonate-labeled cells shows that the rate of turnover of the isoprenoid group from prelamin A is comparable to the rate of conversion of prelamin A to lamin A. These results suggest that during the proteolytic maturation of prelamin A, the isoprenylated moiety is lost. A significant difference between prelamin A processing, and that of p21ras and the B-type lamins that undergo isoprenylation-dependent proteolytic maturation, is that the mature form of lamin A is no longer isoprenylated

Laminates

Science.gov (United States)

Lepedat, Karin; Wagner, Robert; Lang, Jürgen

The use of phenolic resin for the impregnation of a carrier material such as paper or fabric based on either organic or inorganic fibers was and still is one of the most important application areas for liquid phenolic resins. Substrates like paper, cotton, or glass fabric impregnated with phenolic resins are used as core layers for decorative and technical laminates and for many other different industrial applications. Nowadays, phenolic resins for decorative laminates used for furniture, flooring, or in the construction and transportation industry have gained significant market share. The Laminates chapter mainly describes the manufacture of decorative laminates especially the impregnation and pressing process with special emphasis to new technological developments and recent trends. Moreover, the different types of laminates are introduced, combined with some brief comments as they relate to the market for decorative surfaces.

Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection

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Wenqiang Wei

2016-05-01

Full Text Available At present, the details of lamina alterations after baculovirus infection remain elusive. In this study, a lamin gene in the Sf9 cell line of Spodoptera frugiperda was cloned. The open reading frame (orf of the Sf9 lamin was 1860 bp and encoded a protein with a molecular weight of 70 kDa. A transfection assay with a red fluorescence protein (rfp-lamin fusion protein indicated that Sf9 lamin was localized in the nuclear rim. Transmission electron microscopy observations indicated that Autographa californica multiple nucleopolyhedrovirus (AcMNPV nucleocapsids may pass through the nuclear envelope. Immunofluorescence assay indicated that the lamina showed a ruffled staining pattern with the formation of invaginations in the Sf9 cells infected with AcMNPV, while it was evenly distributed at the nuclear periphery of mock-infected cells. Western blotting results indicated that the total amount of lamin in the baculovirus-infected Sf9 cells was significantly decreased compared with the mock-infected cells. These results imply that AcMNPV infection induces structural and biochemical rearrangements of lamina of Sf9 cells.

Significance of host cell kinases in herpes simplex virus type 1 egress and lamin-associated protein disassembly from the nuclear lamina

International Nuclear Information System (INIS)

Leach, Natalie R.; Roller, Richard J.

2010-01-01

The nuclear lamina is thought to be a steric barrier to the herpesvirus capsid. Disruption of the lamina accompanied by phosphorylation of lamina proteins is a conserved feature of herpesvirus infection. In HSV-1-infected cells, protein kinase C (PKC) alpha and delta isoforms are recruited to the nuclear membrane and PKC delta has been implicated in phosphorylation of emerin and lamin B. We tested two critical hypotheses about the mechanism and significance of lamina disruption. First, we show that chemical inhibition of all PKC isoforms reduced viral growth five-fold and inhibited capsid egress from the nucleus. However, specific inhibition of either conventional PKCs or PKC delta does not inhibit viral growth. Second, we show hyperphosphorylation of emerin by viral and cellular kinases is required for its disassociation from the lamina. These data support hypothesis that phosphorylation of lamina components mediates lamina disruption during HSV nuclear egress.

Influence of specimen type and reinforcement on measured tension-tension fatigue life of unidirectional GFRP laminates

DEFF Research Database (Denmark)

Korkiakoski, Samuli; Brøndsted, Povl; Sarlin, Essi

2016-01-01

It is well known that standardised tension-tension fatigue test specimens of unidirectional (UD) glass-fibre-reinforced plastics (GFRP) laminates tend to fail at end tabs. The true fatigue life is then underestimated. The first objective of this study was to find for UD GFRP laminates a test...... specimen that fails in the gauge section. The second objective was to compare fatigue performance of two laminates, one having a newly developed UD powder-bound fabric as a reinforcement and the other having a quasi-UD stitched non-crimp fabric as a reinforcement. In the first phase, a rectangular specimen...... in accordance with the ISO 527-5 standard and two slightly different dog-bone shaped specimens were evaluated by means of finite element modelling. Subsequent comparative fatigue tests were performed for the laminates with the three specimen types. The results showed that the test specimen type has...

Lamins of the sea lamprey (Petromyzon marinus) and the evolution of the vertebrate lamin protein family.

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Schilf, Paul; Peter, Annette; Hurek, Thomas; Stick, Reimer

2014-07-01

Lamin proteins are found in all metazoans. Most non-vertebrate genomes including those of the closest relatives of vertebrates, the cephalochordates and tunicates, encode only a single lamin. In teleosts and tetrapods the number of lamin genes has quadrupled. They can be divided into four sub-types, lmnb1, lmnb2, LIII, and lmna, each characterized by particular features and functional differentiations. Little is known when during vertebrate evolution these features have emerged. Lampreys belong to the Agnatha, the sister group of the Gnathostomata. They split off first within the vertebrate lineage. Analysis of the sea lamprey (Petromyzon marinus) lamin complement presented here, identified three functional lamin genes, one encoding a lamin LIII, indicating that the characteristic gene structure of this subtype had been established prior to the agnathan/gnathostome split. Two other genes encode lamins for which orthology to gnathostome lamins cannot be designated. Search for lamin gene sequences in all vertebrate taxa for which sufficient sequence data are available reveals the evolutionary time frame in which specific features of the vertebrate lamins were established. Structural features characteristic for A-type lamins are not found in the lamprey genome. In contrast, lmna genes are present in all gnathostome lineages suggesting that this gene evolved with the emergence of the gnathostomes. The analysis of lamin gene neighborhoods reveals noticeable similarities between the different vertebrate lamin genes supporting the hypothesis that they emerged due to two rounds of whole genome duplication and makes clear that an orthologous relationship between a particular vertebrate paralog and lamins outside the vertebrate lineage cannot be established. Copyright © 2014 Elsevier GmbH. All rights reserved.

Aberrant localization of lamin B receptor (LBR) in cellular senescence in human cells

Energy Technology Data Exchange (ETDEWEB)

Arai, Rumi; En, Atsuki; Ukekawa, Ryo [Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027 (Japan); Miki, Kensuke [Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027 (Japan); Ichiban Life Corporation, 1-1-7 Horai-cho, Naka-ku, Yokohama 231-0048 (Japan); Fujii, Michihiko, E-mail: mifuji@yokohama-cu.ac.jp [Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027 (Japan); Ayusawa, Dai [Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027 (Japan); Ichiban Life Corporation, 1-1-7 Horai-cho, Naka-ku, Yokohama 231-0048 (Japan)

2016-05-13

5-Bromodeoxyuridine (BrdU), a thymidine analogue, induces cellular senescence in mammalian cells. BrdU induces cellular senescence probably through the regulation of chromatin because BrdU destabilizes or disrupts nucleosome positioning and decondenses heterochromatin. Since heterochromatin is tethered to the nuclear periphery through the interaction with the nuclear envelope proteins, we examined the localization of the several nuclear envelope proteins such as lamins, lamin-interacting proteins, nuclear pore complex proteins, and nuclear transport proteins in senescent cells. We have shown here that lamin B receptor (LBR) showed a change in localization in both BrdU-induced and replicative senescent cells.

Hypertonic-induced lamin A/C synthesis and distribution to nucleoplasmic speckles is mediated by TonEBP/NFAT5 transcriptional activator

International Nuclear Information System (INIS)

Favale, Nicolas O.; Sterin Speziale, Norma B.; Fernandez Tome, Maria C.

2007-01-01

Lamin A/C is the most studied nucleoskeletal constituent. Lamin A/C expression indicates cell differentiation and is also a structural component of nuclear speckles, which are involved in gene expression regulation. Hypertonicity has been reported to induce renal epithelial cell differentiation and expression of TonEBP (NFAT5), a transcriptional activator of hypertonicity-induced gene transcription. In this paper, we investigate the effect of hypertonicity on lamin A/C expression in MDCK cells and the involvement of TonEBP. Hypertonicity increased lamin A/C expression and its distribution to nucleoplasm with speckled pattern. Microscopy showed codistribution of TonEBP and lamin A/C in nucleoplasmic speckles, and immunoprecipitation demonstrated their interaction. TonEBP silencing caused lamin A/C redistribution from nucleoplasmic speckles to the nuclear rim, followed by lamin decrease, thus showing that hypertonicity induces lamin A/C speckles through a TonEBP-dependent mechanism. We suggest that lamin A/C speckles could serve TonEBP as scaffold thus favoring its role in hypertonicity

The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes.

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Takashi Shibano

Full Text Available The inner nuclear membrane (INM protein Nemp1/TMEM194A has previously been suggested to be involved in eye development in Xenopus, and contains two evolutionarily conserved sequences in the transmembrane domains (TMs and the C-terminal region, named region A and region B, respectively. To elucidate the molecular nature of Nemp1, we analyzed its interacting proteins through those conserved regions. First, we found that Nemp1 interacts with itself and lamin through the TMs and region A, respectively. Colocalization of Nemp1 and lamin at the INM suggests that the interaction with lamin participates in the INM localization of Nemp1. Secondly, through yeast two-hybrid screening using region B as bait, we identified the small GTPase Ran as a probable Nemp1-binding partner. GST pulldown and co-immunoprecipitation assays using region B and Ran mutants revealed that region B binds directly to the GTP-bound Ran through its effector domain. Immunostaining experiments using transfected COS-7 cells revealed that full-length Nemp1 recruits Ran near the nuclear envelope, suggesting a role for Nemp1 in the accumulation of RanGTP at the nuclear periphery. At the neurula-to-tailbud stages of Xenopus embryos, nemp1 expression overlapped with ran in several regions including the eye vesicles. Co-knockdown using antisense morpholino oligos for nemp1 and ran caused reduction of cell densities and severe eye defects more strongly than either single knockdown alone, suggesting their functional interaction. Finally we show that Arabidopsis thaliana Nemp1-orthologous proteins interact with A. thaliana Ran, suggesting their evolutionally conserved physical and functional interactions possibly in basic cellular functions including nuclear transportation. Taken together, we conclude that Nemp1 represents a new type of RanGTP-binding protein.

DESIGN ALTERNATIVES ON THE LAMINATES

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Gökay Nemli

2004-04-01

Full Text Available Wood based panel manufacturers use increasing volumes of laminates. Laminates are resistant to the water, humidity, scratch, abrasion, burning and chemicals. These products consist of printed decor papers that have been saturated with thermosetting resin. In this study, laminate types, composition form and design alternatives were investigated.

Progressive delamination in polymer matrix composite laminates: A new approach

Science.gov (United States)

Chamis, C. C.; Murthy, P. L. N.; Minnetyan, L.

1992-01-01

A new approach independent of stress intensity factors and fracture toughness parameters has been developed and is described for the computational simulation of progressive delamination in polymer matrix composite laminates. The damage stages are quantified based on physics via composite mechanics while the degradation of the laminate behavior is quantified via the finite element method. The approach accounts for all types of composite behavior, laminate configuration, load conditions, and delamination processes starting from damage initiation, to unstable propagation, and to laminate fracture. Results of laminate fracture in composite beams, panels, plates, and shells are presented to demonstrate the effectiveness and versatility of this new approach.

LamLum : a tool for evaluating the financial feasibility of laminated lumber plants

Science.gov (United States)

E.M. (Ted) Bilek; John F. Hunt

2006-01-01

A spreadsheet-based computer program called LamLum was created to analyze the economics of value- added laminated lumber manufacturing facilities. Such facilities manufacture laminations, typically from lower grades of structural lumber, then glue these laminations together to make various types of higher value laminated lumber products. This report provides the...

Evaluation of shear-compressive strength properties for laminated GFRP composites in electromagnet system

Science.gov (United States)

Song, Jun Hee; Kim, Hak Kun; Kim, Sam Yeon

2014-07-01

Laminated fiber-reinforced composites can be applied to an insulating structure of a nuclear fusion device. It is necessary to investigate the interlaminar fracture characteristics of the laminated composites for the assurance of design and structural integrity. The three methods used to prepare the glass fiber reinforced plastic composites tested in this study were vacuum pressure impregnation, high pressure laminate (HPL), and prepreg laminate. We discuss the design criteria for safe application of composites and the shear-compressive test methods for evaluating mechanical properties of the material. Shear-compressive tests could be performed successfully using series-type test jigs that were inclined 0°, 30°, 45°, 60°, and 75° to the normal axis. Shear strength depends strongly on the applied compressive stress. The design range of allowable shear stress was extended by use of the appropriate composite fabrication method. HPL had the largest design range, and the allowable interlaminar shear stress was 0.254 times the compressive stress.

Experimental and Numerical Investigation of Metal Type and Thickness Effects on the Impact Resistance of Fiber Metal Laminates

NARCIS (Netherlands)

Sadighi, M.; Pärnänen, T.; Alderliesten, R.C.; Sayeaftabi, M.; Benedictus, R.

2012-01-01

The impact response of fiber metal laminates (FMLs), has been investigated with experiments and numerical simulations, which is reported in this article. Low-velocity impacts were carried out to study the effects of metal type and thickness within FMLs. Glare5-3/2 laminates with two aluminum layer

Differentiation of C2C12 myoblasts expressing lamin A mutated at a site responsible for Emery-Dreifuss muscular dystrophy is improved by inhibition of the MEK-ERK pathway and stimulation of the PI3-kinase pathway

International Nuclear Information System (INIS)

Favreau, Catherine; Delbarre, Erwan; Courvalin, Jean-Claude; Buendia, Brigitte

2008-01-01

Mutation R453W in A-type lamins, that are major nuclear envelope proteins, generates Emery-Dreifuss muscular dystrophy. We previously showed that mouse myoblasts expressing R453W-lamin A incompletely exit the cell cycle and differentiate into myocytes with a low level of multinucleation. Here we attempted to improve differentiation by treating these cells with a mixture of PD98059, an extracellular-regulated kinase (ERK) kinase (also known as mitogen-activated kinase, MEK) inhibitor, and insulin-like growth factor-II, an activator of phosphoinositide 3-kinase. We show that mouse myoblasts expressing R453W-lamin A were sensitive to the drug treatment as shown by (i) an increase in multinucleation, (ii) downregulation of proliferation markers (cyclin D1, hyperphosphorylated Rb), (iii) upregulation of myogenin, and (iv) sustained activation of p21 and cyclin D3. However, nuclear matrix anchorage of p21 and cyclin D3 in a complex with hypophosphorylated Rb that is critical to trigger cell cycle arrest and myogenin induction was deficient and incompletely restored by drug treatment. As the turn-over of R453W-lamin A at the nuclear envelope was greatly enhanced, we propose that R453W-lamin A impairs the capacity of the nuclear lamina to serve as scaffold for substrates of the MEK-ERK pathway and for MyoD-induced proteins that play a role in the differentiation process

Development of High Performance CFRP/Metal Active Laminates

Science.gov (United States)

Asanuma, Hiroshi; Haga, Osamu; Imori, Masataka

This paper describes development of high performance CFRP/metal active laminates mainly by investigating the kind and thickness of the metal. Various types of the laminates were made by hot-pressing of an aluminum, aluminum alloys, a stainless steel and a titanium for the metal layer as a high CTE material, a unidirectional CFRP prepreg as a low CTE/electric resistance heating material, a unidirectional KFRP prepreg as a low CTE/insulating material. The aluminum and its alloy type laminates have almost the same and the highest room temperature curvatures and they linearly change with increasing temperature up to their fabrication temperature. The curvature of the stainless steel type jumps from one to another around its fabrication temperature, whereas the titanium type causes a double curvature and its change becomes complicated. The output force of the stainless steel type attains the highest of the three under the same thickness. The aluminum type successfully increased its output force by increasing its thickness and using its alloys. The electric resistance of the CFRP layer can be used to monitor the temperature, that is, the curvature of the active laminate because the curvature is a function of temperature.

Impact damages modeling in laminated composite structures

Directory of Open Access Journals (Sweden)

Kreculj Dragan D.

2014-01-01

Full Text Available Laminated composites have an important application in modern engineering structures. They are characterized by extraordinary properties, such as: high strength and stiffness and lightweight. Nevertheless, a serious obstacle to more widespread use of those materials is their sensitivity to the impact loads. Impacts cause initiation and development of certain types of damages. Failures that occur in laminated composite structures can be intralaminar and interlaminar. To date it was developed a lot of simulation models for impact damages analysis in laminates. Those models can replace real and expensive testing in laminated structures with a certain accuracy. By using specialized software the damage parameters and distributions can be determined (at certain conditions on laminate structures. With performing numerical simulation of impact on composite laminates there are corresponding results valid for the analysis of these structures.

OGT (O-GlcNAc Transferase) Selectively Modifies Multiple Residues Unique to Lamin A.

Science.gov (United States)

Simon, Dan N; Wriston, Amanda; Fan, Qiong; Shabanowitz, Jeffrey; Florwick, Alyssa; Dharmaraj, Tejas; Peterson, Sherket B; Gruenbaum, Yosef; Carlson, Cathrine R; Grønning-Wang, Line M; Hunt, Donald F; Wilson, Katherine L

2018-05-17

The LMNA gene encodes lamins A and C with key roles in nuclear structure, signaling, gene regulation, and genome integrity. Mutations in LMNA cause over 12 diseases ('laminopathies'). Lamins A and C are identical for their first 566 residues. However, they form separate filaments in vivo, with apparently distinct roles. We report that lamin A is β- O -linked N -acetylglucosamine- (O -GlcNAc)-modified in human hepatoma (Huh7) cells and in mouse liver. In vitro assays with purified O -GlcNAc transferase (OGT) enzyme showed robust O -GlcNAcylation of recombinant mature lamin A tails (residues 385⁻646), with no detectable modification of lamin B1, lamin C, or 'progerin' (Δ50) tails. Using mass spectrometry, we identified 11 O -GlcNAc sites in a 'sweet spot' unique to lamin A, with up to seven sugars per peptide. Most sites were unpredicted by current algorithms. Double-mutant (S612A/T643A) lamin A tails were still robustly O -GlcNAc-modified at seven sites. By contrast, O -GlcNAcylation was undetectable on tails bearing deletion Δ50, which causes Hutchinson⁻Gilford progeria syndrome, and greatly reduced by deletion Δ35. We conclude that residues deleted in progeria are required for substrate recognition and/or modification by OGT in vitro. Interestingly, deletion Δ35, which does not remove the majority of identified O -GlcNAc sites, does remove potential OGT-association motifs (lamin A residues 622⁻625 and 639⁻645) homologous to that in mouse Tet1. These biochemical results are significant because they identify a novel molecular pathway that may profoundly influence lamin A function. The hypothesis that lamin A is selectively regulated by OGT warrants future testing in vivo, along with two predictions: genetic variants may contribute to disease by perturbing OGT-dependent regulation, and nutrient or other stresses might cause OGT to misregulate wildtype lamin A.

Attenuated hypertrophic response to pressure overload in a lamin A/C haploinsufficiency mouse.

Science.gov (United States)

Cupesi, Mihaela; Yoshioka, Jun; Gannon, Joseph; Kudinova, Anastacia; Stewart, Colin L; Lammerding, Jan

2010-06-01

Inherited mutations cause approximately 30% of all dilated cardiomyopathy cases, with autosomal dominant mutations in the LMNA gene accounting for more than one third of these. The LMNA gene encodes the nuclear envelope proteins lamins A and C, which provide structural support to the nucleus and also play critical roles in transcriptional regulation. Functional deletion of a single allele is sufficient to trigger dilated cardiomyopathy in humans and mice. However, whereas Lmna(-/-) mice develop severe muscular dystrophy and dilated cardiomyopathy and die by 8 weeks of age, heterozygous Lmna(+/-) mice have a much milder phenotype, with changes in ventricular function and morphology only becoming apparent at 1 year of age. Here, we studied 8- to 20-week-old Lmna(+/-) mice and wild-type littermates in a pressure overload model to examine whether increased mechanical load can accelerate or exacerbate myocardial dysfunction in the heterozygotes. While overall survival was similar between genotypes, Lmna(+/-) animals had a significantly attenuated hypertrophic response to pressure overload as evidenced by reduced ventricular mass and myocyte size. Analysis of pressure overload-induced transcriptional changes suggested that the reduced hypertrophy in the Lmna(+/-) mice was accompanied by impaired activation of the mechanosensitive gene Egr-1. In conclusion, our findings provide further support for a critical role of lamins A and C in regulating the cellular response to mechanical stress in cardiomyocytes and demonstrate that haploinsufficiency of lamins A and C alone is sufficient to alter hypertrophic responses and cardiac function in the face of pressure overload in the heart. (c) 2009 Elsevier Ltd. All rights reserved.

A historical perspective of laminitis.

Science.gov (United States)

Heymering, Henry W

2010-04-01

The causes of laminitis are many-often interrelated, sometimes direct opposites. The history of laminitis has been a search for the cause or causes of laminitis and for effective treatment. Going in and out of fashion, many treatments have lasted for centuries, some for millennia, but very few have been proven. Copyright 2010 Elsevier Inc. All rights reserved.

Lamination cooling system

Science.gov (United States)

Rippel, Wally E.; Kobayashi, Daryl M.

2005-10-11

An electric motor, transformer or inductor having a lamination cooling system including a stack of laminations, each defining a plurality of apertures at least partially coincident with apertures of adjacent laminations. The apertures define a plurality of cooling-fluid passageways through the lamination stack, and gaps between the adjacent laminations are sealed to prevent a liquid cooling fluid in the passageways from escaping between the laminations. The gaps are sealed by injecting a heat-cured sealant into the passageways, expelling excess sealant, and heat-curing the lamination stack. The apertures of each lamination can be coincident with the same-sized apertures of adjacent laminations to form straight passageways, or they can vary in size, shape and/or position to form non-axial passageways, angled passageways, bidirectional passageways, and manifold sections of passageways that connect a plurality of different passageway sections. Manifold members adjoin opposite ends of the lamination stack, and each is configured with one or more cavities to act as a manifold to adjacent passageway ends. Complex manifold arrangements can create bidirectional flow in a variety of patterns.

Lamin A expression in circulating osteoprogenitors as a potential biomarker for frailty: The Nepean Osteoporosis and Frailty (NOF) Study.

Science.gov (United States)

Al Saedi, Ahmed; Gunawardene, Piumali; Bermeo, Sandra; Vogrin, Sara; Boersma, Derek; Phu, Steven; Singh, Lakshman; Suriyaarachchi, Pushpa; Duque, Gustavo

2018-02-01

Lamin A is a protein of the nuclear lamina. Low levels of lamin A expression are associated with osteosarcopenia in mice. In this study, we hypothesized that low lamin A expression is also associated with frailty in humans. We aimed to develop a non-invasive method to quantify lamin A expression in epithelial and circulating osteoprogenitor (COP) cells, and to determine the relationship between lamin A expression and frailty in older individuals. COP cells and buccal swabs were obtained from 66 subjects (median age 74; 60% female; 26 non-frail, 23 pre-frail, and 17 frail) participating at the Nepean Osteoporosis and Frailty (NOF) Study. We quantified physical performance and disability, and stratified frailty in this population. Lamin A expression in epithelial and COP cells was quantified by flow cytometry. Linear regression models estimated the relationship between lamin A expression in buccal and COP cells, and prevalent disability and frailty. Lamin A expression in buccal cells showed no association with either disability or frailty. Low lamin A expression values in COP cells were associated with frailty. Frail individuals showed 60% lower levels of lamin A compared to non-frail (95% CI -36 to -74%, p<0.001) and 62% lower levels compared to pre-frail (95%CI -40 to -76%, p<0.001). In summary, we have identified lamin A expression in COP cells as a strong indicator of frailty. Further work is needed to understand lamin A expression as a risk stratifier, biomarker, or therapeutic target in frail older persons. Copyright © 2017 Elsevier Inc. All rights reserved.

Investigation into the Quality of Thermally Treated Package Lamination

Directory of Open Access Journals (Sweden)

Darius Kazlauskas

2011-02-01

Full Text Available The article deals with the problem of delaminating the package after pasteurization at relatively high temperatures. The main parameters of the lamination process influencing lamination strength were determined. The role of the amount of lamination glue and tension in the rewinder for two glue types were experimentally examined defining lamination regimes at which the process of delamination is excluded.Article in Lithuanian

Nuclear lamina and nuclear matrix organization in sperm pronuclei assembled in Xenopus egg extract.

Science.gov (United States)

Zhang, C; Jenkins, H; Goldberg, M W; Allen, T D; Hutchison, C J

1996-09-01

Nuclear lamina and matrices were prepared from sperm pronuclei assembled in Xenopus egg extracts using a fractionation and extraction procedure. Indirect immunofluorescence revealed that while chromatin was efficiently removed from nuclei during the extraction procedure, the distribution of lamins was unaffected. Consistent with this data, the amount of lamin B3, determined by immunoblotting, was not affected through the extraction procedure. Nuclear matrices were visualised in DGD sections by TEM. Within these sections filaments were observed both at the boundary of the nucleus (the lamina) and within the body of the nucleus (internal nuclear matrix filaments). To improve resolution, nuclear matrices were also prepared as whole mounts and viewed using field emission in lens scanning electron microscopy (FEISEM). This technique revealed two distinct networks of filaments. Filaments lying at the surface of nuclear matrices interconnected nuclear pores. These filaments were readily labelled with monoclonal anti-lamin B3 antibodies. Filaments lying within the body of the nuclear matrix were highly branched but were not readily labelled with antilamin B3 antibodies. Nuclear matrices were also prepared from sperm pronuclei assembled in lamin B3 depleted extracts. Using FEISEM, filaments were also detected in these preparations. However, these filaments were poorly organised and often appeared to aggregate. To confirm these results nuclear matrices were also observed as whole mounts using TEM. Nuclear matrices prepared from control nuclei contained a dense array of interconnected filaments. Many (but not all) of these filaments were labelled with anti-lamin B3 antibodies. In contrast, nuclear matrices prepared from "lamin depleted nuclei' contained poorly organised or aggregated filaments which were not specifically labelled with anti-lamin B3 antibodies.

Equivalent Circuit Model of Low-Frequency Magnetoelectric Effect in Disk-Type Terfenol-D/PZT Laminate Composites Considering a New Interface Coupling Factor.

Science.gov (United States)

Lou, Guofeng; Yu, Xinjie; Lu, Shihua

2017-06-15

This paper describes the modeling of magnetoelectric (ME) effects for disk-type Terfenol-D (Tb 0.3 Dy 0.7 Fe 1.92 )/PZT (Pb(Zr,Ti)O₃) laminate composite at low frequency by combining the advantages of the static elastic model and the equivalent circuit model, aiming at providing a guidance for the design and fabrication of the sensors based on magnetoelectric laminate composite. Considering that the strains of the magnetostrictive and piezoelectric layers are not equal in actual operating due to the epoxy resin adhesive bonding condition, the magnetostrictive and piezoelectric layers were first modeled through the equation of motion separately, and then coupled together with a new interface coupling factor k c , which physically reflects the strain transfer between the phases. Furthermore, a theoretical expression containing k c for the transverse ME voltage coefficient α v and the optimum thickness ratio n optim to which the maximum ME voltage coefficient corresponds were derived from the modified equivalent circuit of ME laminate, where the interface coupling factor acted as an ideal transformer. To explore the influence of mechanical load on the interface coupling factor k c , two sets of weights, i.e., 100 g and 500 g, were placed on the top of the ME laminates with the same thickness ratio n in the sample fabrication. A total of 22 T-T mode disk-type ME laminate samples with different configurations were fabricated. The interface coupling factors determined from the measured α v and the DC bias magnetic field H bias were 0.11 for 500 g pre-mechanical load and 0.08 for 100 g pre-mechanical load. Furthermore, the measured optimum thickness ratios were 0.61 for k c = 0.11 and 0.56 for k c = 0.08. Both the theoretical ME voltage coefficient α v and optimum thickness ratio n optim containing k c agreed well with the measured data, verifying the reasonability and correctness for the introduction of k c in the modified equivalent circuit model.

Equivalent Circuit Model of Low-Frequency Magnetoelectric Effect in Disk-Type Terfenol-D/PZT Laminate Composites Considering a New Interface Coupling Factor

Directory of Open Access Journals (Sweden)

Guofeng Lou

2017-06-01

Full Text Available This paper describes the modeling of magnetoelectric (ME effects for disk-type Terfenol-D (Tb0.3Dy0.7Fe1.92/PZT (Pb(Zr,TiO3 laminate composite at low frequency by combining the advantages of the static elastic model and the equivalent circuit model, aiming at providing a guidance for the design and fabrication of the sensors based on magnetoelectric laminate composite. Considering that the strains of the magnetostrictive and piezoelectric layers are not equal in actual operating due to the epoxy resin adhesive bonding condition, the magnetostrictive and piezoelectric layers were first modeled through the equation of motion separately, and then coupled together with a new interface coupling factor kc, which physically reflects the strain transfer between the phases. Furthermore, a theoretical expression containing kc for the transverse ME voltage coefficient αv and the optimum thickness ratio noptim to which the maximum ME voltage coefficient corresponds were derived from the modified equivalent circuit of ME laminate, where the interface coupling factor acted as an ideal transformer. To explore the influence of mechanical load on the interface coupling factor kc, two sets of weights, i.e., 100 g and 500 g, were placed on the top of the ME laminates with the same thickness ratio n in the sample fabrication. A total of 22 T-T mode disk-type ME laminate samples with different configurations were fabricated. The interface coupling factors determined from the measured αv and the DC bias magnetic field Hbias were 0.11 for 500 g pre-mechanical load and 0.08 for 100 g pre-mechanical load. Furthermore, the measured optimum thickness ratios were 0.61 for kc = 0.11 and 0.56 for kc = 0.08. Both the theoretical ME voltage coefficient αv and optimum thickness ratio noptim containing kc agreed well with the measured data, verifying the reasonability and correctness for the introduction of kc in the modified equivalent circuit model.

An essential GT motif in the lamin A promoter mediates activation by CREB-binding protein

International Nuclear Information System (INIS)

Janaki Ramaiah, M.; Parnaik, Veena K.

2006-01-01

Lamin A is an important component of nuclear architecture in mammalian cells. Mutations in the human lamin A gene lead to highly degenerative disorders that affect specific tissues. In studies directed towards understanding the mode of regulation of the lamin A promoter, we have identified an essential GT motif at -55 position by reporter gene assays and mutational analysis. Binding of this sequence to Sp transcription factors has been observed in electrophoretic mobility shift assays and by chromatin immunoprecipitation studies. Further functional analysis by co-expression of recombinant proteins and ChIP assays has shown an important regulatory role for CREB-binding protein in promoter activation, which is mediated by the GT motif

Laminated articles

International Nuclear Information System (INIS)

Ridgway, P.C.; Case, D.F.

1979-01-01

In a method of bonding laminations of a magnetic core, photo-resist material consisting of a co-polymer is applied as a film to a sheet of magnetic material to define lamination shapes to enable the laminations to be formed by etching. The film of photo-resist material on the laminations is then utilised to bond the laminations together in a stack. In order to permit the core to operate at temperatures higher than the softening temperature of the photo-resist material, the bonded stack is irradiated with 1 - 2 Mer gamma radiation to a dose of 1 - 5 Mrads in 2 - 10 hrs to cause changes to the bonding material such that the material does not soften at the operating temperature of the core. (U.K.)

Investigation of the Mechanical Properties of Hybrid Carbon-Hemp Laminated Composites Used as Thermal Insulation for Different Industrial Applications

Directory of Open Access Journals (Sweden)

M. L. Scutaru

2014-04-01

Full Text Available Carbon-hemp composite laminate provides good thermal properties. For this reason this type of material is presently being used for various applications like insulator for airplanes, spaceships, nuclear reactors, and so forth. Unfortunately their mechanical properties are less studied. These characteristics are very important since they should be guaranteed also for important mechanical stress in addition to the thermal one. The present paper presents a study regarding the impact testing of some hybrid composite laminate panels based on polyester resin reinforced with both carbon and hemp fabric. The effects of different impact speeds on the mechanical behavior of these panels have been analyzed. The paper lays stress on the characterization of this hybrid composite laminate regarding the impact behavior of these panels by dropping a weight with low velocity.

Study on mechanical properties of laminated rubber bearing with small shape factor

International Nuclear Information System (INIS)

Mazda, T.; Ootori, Y.; Yabana, S.; Hirata, K.; Ishida, K.

1995-01-01

Laminated rubber bearings with a small shape factor are regarded as one of the most promising isolation devices to reduce the vertical seismic load of a nuclear power plant. In this study, three types of natural rubber bearings with different aspect ratios (diameter/total thickness of rubber) and one high-damping rubber bearing are tested under varied loading conditions. Basic characteristics and ultimate characteristics of the bearings are made clear, and applicability of estimation formula and analysis method are verified

The mechanical behavior of GLARE laminates for aircraft structures

Science.gov (United States)

Wu, Guocai; Yang, J.-M.

2005-01-01

GLARE (glass-reinforced aluminum laminate) is a new class of fiber metal laminates for advanced aerospace structural applications. It consists of thin aluminum sheets bonded together with unidirectional or biaxially reinforced adhesive prepreg of high-strength glass fibers. GLARE laminates offer a unique combination of properties such as outstanding fatigue resistance, high specific static properties, excellent impact resistance, good residual and blunt notch strength, flame resistance and corrosion properties, and ease of manufacture and repair. GLARE laminates can be tailored to suit a wide variety of applications by varying the fiber/resin system, the alloy type and thickness, stacking sequence, fiber orientation, surface pretreatment technique, etc. This article presents a comprehensive overview of the mechanical properties of various GLARE laminates under different loading conditions.

Development of active CFRP/metal laminates and their demonstrations in complicated forms

Science.gov (United States)

Asanuma, H.; Nakata, T.; Tanaka, T.; Imori, M.; Haga, O.

2006-03-01

This paper describes development of high performance CFRP/metal active laminates and demonstrations of them in complicated forms. Various types of the laminates were made by hot-pressing of an aluminum, aluminum alloys, a stainless steel and a titanium for the metal layer as a high CTE material, a unidirectional CFRP prepreg as a low CTE/electric resistance heating material, a unidirectional KFRP prepreg as a low CTE/insulating material. The aluminum and its alloy type laminates have almost the same and the highest room temperature curvatures and they linearly change with increasing temperature up to their fabrication temperature. The curvature of the stainless steel type jumps from one to another around its fabrication temperature, whereas the titanium type causes a double curvature and its change becomes complicated. The output force of the stainless steel type attains the highest of the three under the same thickness. The aluminum type successfully increased its output force by increasing its thickness and using its alloys. The electric resistance of the CFRP layer can be used to monitor the temperature, that is, the curvature of the active laminate because the curvature is a function of temperature. The aluminum type active laminate was made into complicated forms, that is, a hatch, a stack, a coil and a lift types, and their actuation performances were successfully demonstrated.

Formation of Nuclear Splicing Factor Compartments Is Independent of Lamins A/C

Czech Academy of Sciences Publication Activity Database

Večeřová, Jaromíra; Koberna, Karel; Malínský, Jan; Soutoglou, E.; Sullivan, T.; Stewart, C. L.; Raška, Ivan; Misteli, T.

2004-01-01

Roč. 15, č. 11 (2004), s. 4904-4910 ISSN 1059-1524 R&D Projects: GA AV ČR IAA5039103; GA ČR GA304/02/0342 Institutional research plan: CEZ:AV0Z5039906 Keywords : lamins A/C Subject RIV: EA - Cell Biology Impact factor: 7.517, year: 2004

Role of the nuclear envelope in the pathogenesis of age-related bone loss and osteoporosis

Science.gov (United States)

Vidal, Christopher; Bermeo, Sandra; Fatkin, Diane; Duque, Gustavo

2012-01-01

The nuclear envelope is the most important border in the eukaryotic cell. The role of the nuclear envelope in cell differentiation and function is determined by a constant interaction between the elements of the nuclear envelope and the transcriptional regulators involved in signal transcription pathways. Among those components of the nuclear envelope, there is a growing evidence that changes in the expression of A-type lamins, which are essential components of the nuclear lamina, are associated with age-related changes in bone affecting the capacity of differentiation of mesenchymal stem cells into osteoblasts, favoring adipogenesis and affecting the function and survival of the osteocytes. Overall, as A-type lamins are considered as the 'guardians of the soma', these proteins are also essential for the integrity and quality of the bone and pivotal for the longevity of the musculoskeletal system. PMID:23951459

The innate immunity adaptor SARM translocates to the nucleus to stabilize lamins and prevent DNA fragmentation in response to pro-apoptotic signaling.

Directory of Open Access Journals (Sweden)

Chad R Sethman

Full Text Available Sterile alpha and armadillo-motif containing protein (SARM, a highly conserved and structurally unique member of the MyD88 family of Toll-like receptor adaptors, plays an important role in innate immunity signaling and apoptosis. Its exact mechanism of intracellular action remains unclear. Apoptosis is an ancient and ubiquitous process of programmed cell death that results in disruption of the nuclear lamina and, ultimately, dismantling of the nucleus. In addition to supporting the nuclear membrane, lamins serve important roles in chromatin organization, epigenetic regulation, transcription, nuclear transport, and mitosis. Mutations and other damage that destabilize nuclear lamins (laminopathies underlie a number of intractable human diseases. Here, we report that SARM translocates to the nucleus of human embryonic kidney cells by using its amino-terminal Armadillo repeat region. Within the nucleus, SARM forms a previously unreported lattice akin to the nuclear lamina scaffold. Moreover, we show that SARM protects lamins from apoptotic degradation and reduces internucleosomal DNA fragmentation in response to signaling induced by the proinflammatory cytokine Tumor Necrosis Factor alpha. These findings indicate an important link between the innate immunity adaptor SARM and stabilization of nuclear lamins during inflammation-driven apoptosis in human cells.

Bovine laminitis: clinical aspects, pathology and pathogenesis with reference to acute equine laminitis.

Science.gov (United States)

Boosman, R; Németh, F; Gruys, E

1991-07-01

This review deals with the features of clinical and subclinical laminitis in cattle. Prominent clinical signs of acute laminitis are a tender gait and arched back. The sole horn reveals red and yellowish discolourations within five days. In subacute and chronic cases clinical signs are less severe. In chronic laminitis the shape of the claws is altered. Laminitis is frequently followed by sole ulceration and white zone lesions. Blood tests showed no significant changes for laminitic animals. Arteriographic studies of claws affected by laminitis indicated that blood vessels had narrowed lumens. Gross pathology revealed congestion of the corium and rotation of the distal phalanx. Histopathologic studies indicate that laminitis is associated with changes of the vasculature. Peripartum management and nutrition are important factors in its aetiology. It is hypothesised that laminitis is evoked by disturbed digital circulation. In the pathogenesis of acute laminitis three factors are considered important: the occurrence of thrombosis, haemodynamic aspects of the corium, and endotoxins which trigger these pathologic events.

Inhibition of Prenylation Promotes Caspase 3 Activation, Lamin B Degradation and Loss in Metabolic Cell Viability in Pancreatic β-Cells

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Khadija G. Syeda

2017-10-01

Full Text Available Background/Aims: Lamins are intermediate filament proteins that constitute the main components of the lamina underlying the inner-nuclear membrane and serve to organize chromatin. Lamins (e.g., lamin B undergo posttranslational modifications (e.g., isoprenylation at their C-terminal cysteine residues. Such modifications are thought to render optimal association of lamins with the nuclear envelop. Using human islets, rodent islets, and INS-1 832/13 cells, we recently reported significant metabolic defects under glucotoxic and endoplasmic reticulum (ER stress conditions, including caspase 3 activation and lamin B degradation. The current study is aimed at further understanding the regulatory roles of protein prenylation in the induction of the aforestated metabolic defects. Methods: Subcellular phase partitioning assay was done using Triton X-114. Cell morphology and metabolic cell viability assays were carried out using standard methodologies. Results: We report that exposure of pancreatic β-cells to Simvastatin, an inhibitor of mevalonic acid (MVA biosynthesis, and its downstream isoprenoid derivatives, or FTI-277, an inhibitor of farnesyltransferase that mediates farnesylation of lamins, leads to activation of caspase 3 and lamin B degradation. Furthermore, Simvastatin-treatment increased activation of p38MAPK (a stress kinase and inhibited ERK1/2 (regulator of cell proliferation. Inhibition of farnesylation also resulted in the release of degraded lamin B into the cytosolic fraction and promoted loss in metabolic cell viability. Conclusion: Based on these findings we conclude that protein prenylation plays key roles in islet β-cell function. These findings affirm further support to the hypothesis that defects in prenylation pathway induce caspase-3 activation and nuclear lamin degradation in pancreatic β-cells under the duress of metabolic stress (e.g., glucotoxicity.

Characterization of a 65 kDa NIF in the nuclear matrix of the monocot Allium cepa that interacts with nuclear spectrin-like proteins.

Science.gov (United States)

Pérez-Munive, Clara; Blumenthal, Sonal S D; de la Espina, Susana Moreno Díaz

2012-01-01

Plant cells have a well organized nucleus and nuclear matrix, but lack orthologues of the main structural components of the metazoan nuclear matrix. Although data is limited, most plant nuclear structural proteins are coiled-coil proteins, such as the NIFs (nuclear intermediate filaments) in Pisum sativum that cross-react with anti-intermediate filament and anti-lamin antibodies, form filaments 6-12 nm in diameter in vitro, and may play the role of lamins. We have investigated the conservation and features of NIFs in a monocot species, Allium cepa, and compared them with onion lamin-like proteins. Polyclonal antisera against the pea 65 kDa NIF were used in 1D and 2D Western blots, ICM (imunofluorescence confocal microscopy) and IEM (immunoelectron microscopy). Their presence in the nuclear matrix was analysed by differential extraction of nuclei, and their association with structural spectrin-like proteins by co-immunoprecipitation and co-localization in ICM. NIF is a conserved structural component of the nucleus and its matrix in monocots with Mr and pI values similar to those of pea 65 kDa NIF, which localized to the nuclear envelope, perichromatin domains and foci, and to the nuclear matrix, interacting directly with structural nuclear spectrin-like proteins. Its similarities with some of the proteins described as onion lamin-like proteins suggest that they are highly related or perhaps the same proteins.

Formation of nuclear splicing factor compatments is independent of lamins A/C

Czech Academy of Sciences Publication Activity Database

Večeřová, Jaromíra; Koberna, Karel; Malínský, Jan; Sullivan, T.; Stewart, C. L.; Raška, Ivan; Misteli, T.

2003-01-01

Roč. 1, č. 5 (2003), s. S35 ISSN 1214-021X. [Conference on Cell Biology /5./. České Budějovice, 08.09.2003-10.09.2003] R&D Projects: GA ČR GA304/02/0342; GA ČR GA304/03/1121; GA ČR GA304/01/0729 Institutional research plan: CEZ:MSM 111100003 Keywords : lamins A/C Subject RIV: EA - Cell Biology

Lamination sheet of AA BST magnet

CERN Multimedia

CERN PhotoLab

1979-01-01

The AA had 2 types of bending magnets: BLG (window-frame, long and narrow)and BST (H-type, short and wide). The BST had a very wide aperture, 0.564 m of "good field". To demonstrate the size, the petite AA secretary, Val Mansfield, poses with a lamination sheet. See also 7811105, 7906163, 8006050.

Lamination cooling system formation method

Science.gov (United States)

Rippel, Wally E [Altadena, CA; Kobayashi, Daryl M [Monrovia, CA

2009-05-12

An electric motor, transformer or inductor having a cooling system. A stack of laminations have apertures at least partially coincident with apertures of adjacent laminations. The apertures define straight or angled cooling-fluid passageways through the lamination stack. Gaps between the adjacent laminations are sealed by injecting a heat-cured sealant into the passageways, expelling excess sealant, and heat-curing the lamination stack. Manifold members adjoin opposite ends of the lamination stack, and each is configured with one or more cavities to act as a manifold to adjacent passageway ends. Complex manifold arrangements can create bidirectional flow in a variety of patterns.

Serine 62-Phosphorylated MYC Associates with Nuclear Lamins and Its Regulation by CIP2A Is Essential for Regenerative Proliferation

Directory of Open Access Journals (Sweden)

Kevin Myant

2015-08-01

Full Text Available An understanding of the mechanisms determining MYC’s transcriptional and proliferation-promoting activities in vivo could facilitate approaches for MYC targeting. However, post-translational mechanisms that control MYC function in vivo are poorly understood. Here, we demonstrate that MYC phosphorylation at serine 62 enhances MYC accumulation on Lamin A/C-associated nuclear structures and that the protein phosphatase 2A (PP2A inhibitor protein CIP2A is required for this process. CIP2A is also critical for serum-induced MYC phosphorylation and for MYC-elicited proliferation induction in vitro. Complementary transgenic approaches and an intestinal regeneration model further demonstrated the in vivo importance of CIP2A and serine 62 phosphorylation for MYC activity upon DNA damage. However, targeting of CIP2A did not influence the normal function of intestinal crypt cells. These data underline the importance of nuclear organization in the regulation of MYC phosphorylation, leading to an in vivo demonstration of a strategy for inhibiting MYC activity without detrimental physiological effects.

Differential nuclear remodeling of mammalian somatic cells by Xenopus laevis oocyte and egg cytoplasm

International Nuclear Information System (INIS)

Alberio, Ramiro; Johnson, Andrew D.; Stick, Reimer; Campbell, Keith H.S.

2005-01-01

The mechanisms governing nuclear reprogramming have not been fully elucidated yet; however, recent studies show a universally conserved ability of both oocyte and egg components to reprogram gene expression in somatic cells. The activation of genes associated with pluripotency by oocyte/egg components may require the remodeling of nuclear structures, such that they can acquire the features of early embryos and pluripotent cells. Here, we report on the remodeling of the nuclear lamina of mammalian cells by Xenopus oocyte and egg extracts. Lamin A/C is removed from somatic cells incubated in oocyte and egg extracts in an active process that requires permeable nuclear pores. Removal of lamin A/C is specific, since B-type lamins are not changed, and it is not dependent on the incorporation Xenopus egg specific lamin III. Moreover, transcriptional activity is differentially regulated in somatic cells incubated in the extracts. Pol I and II transcriptions are maintained in cells in oocyte extracts; however, both activities are abolished in egg extracts. Our study shows that components of oocyte and egg extracts can modify the nuclear lamina of somatic cells and that this nuclear remodeling induces a structural change in the nucleus which may have implications for transcriptional activity. These experiments suggest that modifications in the nuclear lamina structure by the removal of somatic proteins and the incorporation of oocyte/egg components may contribute to the reprogramming of somatic cell nuclei and may define a characteristic configuration of pluripotent cells

Robust nuclear lamina-based cell classification of aging and senescent cells.

Science.gov (United States)

Righolt, Christiaan H; van 't Hoff, Merel L R; Vermolen, Bart J; Young, Ian T; Raz, Vered

2011-12-01

Changes in the shape of the nuclear lamina are exhibited in senescent cells, as well as in cells expressing mutations in lamina genes. To identify cells with defects in the nuclear lamina we developed an imaging method that quantifies the intensity and curvature of the nuclear lamina. We show that this method accurately describes changes in the nuclear lamina. Spatial changes in nuclear lamina coincide with redistribution of lamin A proteins and local reduction in protein mobility in senescent cell. We suggest that local accumulation of lamin A in the nuclear envelope leads to bending of the structure. A quantitative distinction of the nuclear lamina shape in cell populations was found between fresh and senescent cells, and between primary myoblasts from young and old donors. Moreover, with this method mutations in lamina genes were significantly distinct from cells with wild-type genes. We suggest that this method can be applied to identify abnormal cells during aging, in in vitro propagation, and in lamina disorders.

Attenuated hypertrophic response to pressure overload in a lamin A/C haploinsufficiency mouse

OpenAIRE

Cupesi, Mihaela; Yoshioka, Jun; Gannon, Joseph; Kudinova, Anastacia; Stewart, Colin L.; Lammerding, Jan

2009-01-01

Inherited mutations cause approximately 30% of all dilated cardiomyopathy cases, with autosomal dominant mutations in the LMNA gene accounting for more than one third of these. The LMNA gene encodes the nuclear envelope proteins lamins A and C, which provide structural support to the nucleus and also play critical roles in transcriptional regulation. Functional deletion of a single allele is sufficient to trigger dilated cardiomyopathy in humans and mice. However, whereas Lmna−/− mice develop...

Emery-Dreifuss Muscular Dystrophy-Associated Mutant Forms of Lamin A Recruit the Stress Responsive Protein Ankrd2 into the Nucleus, Affecting the Cellular Response to Oxidative Stress

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Silvia Angori

2017-05-01

Full Text Available Background: Ankrd2 is a stress responsive protein mainly expressed in muscle cells. Upon the application of oxidative stress, Ankrd2 translocates into the nucleus where it regulates the activity of genes involved in cellular response to stress. Emery-Dreifuss Muscular Dystrophy 2 (EDMD2 is a muscular disorder caused by mutations of the gene encoding lamin A, LMNA. As well as many phenotypic abnormalities, EDMD2 muscle cells also feature a permanent basal stress state, the underlying molecular mechanisms of which are currently unclear. Methods: Experiments were performed in EDMD2-lamin A overexpressing cell lines and EDMD2-affected human myotubes. Oxidative stress was produced by H2O2 treatment. Co-immunoprecipitation, cellular subfractionation and immunofluorescence analysis were used to validate the relation between Ankrd2 and forms of lamin A; cellular sensibility to stress was monitored by the analysis of Reactive Oxygen Species (ROS release and cell viability. Results: Our data demonstrate that oxidative stress induces the formation of a complex between Ankrd2 and lamin A. However, EDMD2-lamin A mutants were able to bind and mislocalize Ankrd2 in the nucleus even under basal conditions. Nonetheless, cells co-expressing Ankrd2 and EDMD2-lamin A mutants were more sensitive to oxidative stress than the Ankrd2-wild type lamin A counterpart. Conclusions: For the first time, we present evidence that in muscle fibers from patients affected by EDMD2, Ankrd2 has an unusual nuclear localization. By introducing a plausible mechanism ruling this accumulation, our data hint at a novel function of Ankrd2 in the pathogenesis of EDMD2-affected cells.

Argonaute2 and LaminB modulate gene expression by controlling chromatin topology.

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Ezequiel Nazer

2018-03-01

Full Text Available Drosophila Argonaute2 (AGO2 has been shown to regulate expression of certain loci in an RNA interference (RNAi-independent manner, but its genome-wide function on chromatin remains unknown. Here, we identified the nuclear scaffolding protein LaminB as a novel interactor of AGO2. When either AGO2 or LaminB are depleted in Kc cells, similar transcription changes are observed genome-wide. In particular, changes in expression occur mainly in active or potentially active chromatin, both inside and outside LaminB-associated domains (LADs. Furthermore, we identified a somatic target of AGO2 transcriptional repression, no hitter (nht, which is immersed in a LAD located within a repressive topologically-associated domain (TAD. Null mutation but not catalytic inactivation of AGO2 leads to ectopic expression of nht and downstream spermatogenesis genes. Depletion of either AGO2 or LaminB results in reduced looping interactions within the nht TAD as well as ectopic inter-TAD interactions, as detected by 4C-seq analysis. Overall, our findings reveal coordination of AGO2 and LaminB function to dictate genome architecture and thereby regulate gene expression.

Numerical evaluation of delamination in CFRP laminates by stereo X-ray pictures

International Nuclear Information System (INIS)

Kunoo, Kazuo; Uda, Nobuhide; Ono, Kousei; Onohara, Kaoru; Takahashi, Toshiaki; Tanaka, Hisahiro.

1989-01-01

This paper presents a method for quantifying damage in composite laminates by stereo X-ray pictures. A three dimensional image of delamination, which is one of the characteristic types of damage in composite laminates can be reconstructed with this method. A digital image processing technique is used to analyze X-ray pictures. Experimental results of reconstructing delaminations in carbon/epoxy cross-ply laminates show that this method is accurate enough for practical usage. (author)

Laminae type and possible mechanisms for the formation of laminated sediments in the Shaban Deep, northern Red Sea

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I. A. Seeberg-Elverfeldt

2005-01-01

Full Text Available Laminated sediments in the Shaban Deep, a brine-filled basin in the northern Red Sea, were analyzed with backscattered electron imagery. Here we present possible mechanisms involved in the formation of laminae of various types and homogenous intervals arising from the detailed investigation of multicore GeoB 7805-1 (26°13.9' N and 35°22.6' E; water depth 1447 m and gravity core GeoB 5836-2 (26°12.61' N, 35°21.56' E; water depth 1475 m. Sediment makeup includes six types: a a laminated structure with alternating light (mainly coccoliths and dark (diatom frustules layers, where the diatom component is indicative of the intra-annual variability between stratification and mixing events; b a pocket-like structure attributed to the sinking of particles within fecal pellets and aggregates; c a matrix of tightly packed diatoms that relates to extended stratification/mixing periods of the water column; d homogenous intervals that result from turbidity deposition; e silt accumulations which origin may lie in agglutinated foraminifers; and f pyrite layers with pyrite formation initiated at the seawater-brine interface.

[Laminitis in cattle: a literature review].

Science.gov (United States)

Lischer, C; Ossent, P

1994-10-01

Worldwide afflictions of the claws belong to the economically important diseases in dairy cattle. The significance of laminitis has gained importance in the last years since the condition is regarded as the most important predisposing factor for the development of lesions such as sole ulcer, white line disease and heel horn erosion. Apart from the clinical stages (acute, subacute, chronic, chronic-recurrent) there is also a subclinical form of laminitis which does not cause lameness. It is characterized by soft yellowish sole and heel horn with haemorrhages in the sole and along the white line. Laminitis is a multifactorial event in which nutrition, genetic disposition and the perinatal period, combined with the associated diseases of high-yielding cows, have a particular significance. Currently, two principally different hypotheses on the pathogenesis are discussed. The generally accepted theory bases on a disturbance in the microcirculation of the corium. According to the other theory the circulatory disturbances are secondary to changes which occur in the horn producing cells of the stratum basale of the epidermis. The predisposing factors and the pathogenesis of laminitis are discussed in the light of possible therapeutic and prophylactic measures.

Modeling and Chaotic Dynamics of the Laminated Composite Piezoelectric Rectangular Plate

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Minghui Yao

2014-01-01

Full Text Available This paper investigates the multipulse heteroclinic bifurcations and chaotic dynamics of a laminated composite piezoelectric rectangular plate by using an extended Melnikov method in the resonant case. According to the von Karman type equations, Reddy’s third-order shear deformation plate theory, and Hamilton’s principle, the equations of motion are derived for the laminated composite piezoelectric rectangular plate with combined parametric excitations and transverse excitation. The method of multiple scales and Galerkin’s approach are applied to the partial differential governing equation. Then, the four-dimensional averaged equation is obtained for the case of 1 : 3 internal resonance and primary parametric resonance. The extended Melnikov method is used to study the Shilnikov type multipulse heteroclinic bifurcations and chaotic dynamics of the laminated composite piezoelectric rectangular plate. The necessary conditions of the existence for the Shilnikov type multipulse chaotic dynamics are analytically obtained. From the investigation, the geometric structure of the multipulse orbits is described in the four-dimensional phase space. Numerical simulations show that the Shilnikov type multipulse chaotic motions can occur. To sum up, both theoretical and numerical studies suggest that chaos for the Smale horseshoe sense in motion exists for the laminated composite piezoelectric rectangular plate.

Static and Dynamic Behavior of High Modulus Hybrid Boron/Glass/Aluminum Fiber Metal Laminates

Science.gov (United States)

Yeh, Po-Ching

2011-12-01

This dissertation presents the investigation of a newly developed hybrid fiber metal laminates (FMLs) which contains commingled boron fibers, glass fibers, and 2024-T3 aluminum sheets. Two types of hybrid boron/glass/aluminum FMLs are developed. The first, type I hybrid FMLs, contained a layer of boron fiber prepreg in between two layers of S2-glass fiber prepreg, sandwiched by two aluminum alloy 2024-T3 sheets. The second, type II hybrid FMLs, contained three layer of commingled hybrid boron/glass fiber prepreg layers, sandwiched by two aluminum alloy 2024-T3 sheets. The mechanical behavior and deformation characteristics including blunt notch strength, bearing strength and fatigue behavior of these two types of hybrid boron/glass/aluminum FMLs were investigated. Compared to traditional S2-glass fiber reinforced aluminum laminates (GLARE), the newly developed hybrid boron/glass/aluminum fiber metal laminates possess high modulus, high yielding stress, and good blunt notch properties. From the bearing test result, the hybrid boron/glass/aluminum fiber metal laminates showed outstanding bearing strength. The high fiber volume fraction of boron fibers in type II laminates lead to a higher bearing strength compared to both type I laminates and traditional GLARE. Both types of hybrid FMLs have improved fatigue crack initiation lives and excellent fatigue crack propagation resistance compared to traditional GLARE. The incorporation of the boron fibers improved the Young's modulus of the composite layer in FMLs, which in turn, improved the fatigue crack initiation life and crack propagation rates of the aluminum sheets. Moreover, a finite element model was established to predict and verify the properties of hybrid boron/glass/aluminum FMLs. The simulated results showed good agreement with the experimental results.

Effect of laminate edge conditions on the formation of microvoids in composite laminates

Science.gov (United States)

Anderson, J. P.; Altan, M. C.

2015-05-01

Manufacturing defects such as microvoids are common in thermoset composite components and are known to negatively affect their strength. The resin pressure developed in and the resin flow out from the laminates during cure have been reported to be the primary factors influencing the final void content of a composite component. In this work, the effect of laminate edge conditions during the cure process on the formation of microvoids was experimentally investigated. This was achieved by fabricating eight-ply laminates from TenCate® BT250/7781 prepreg in a hot-press at a constant cure pressure of 170 kPa while limiting the laminate perimeter available for resin flow by 0%, 25%, 50%, 75%, and 100%. The individual plies of these five laminates were conditioned at 99% relative humidity before curing to maximize the moisture present in the lay-up before fabrication. The presence of moisture in the lay-ups was expected to promote void formation and allow the effect of restricting flow at the edges of a laminate to be better identified. The restriction of resin outflow was found to cause the average characteristic void diameter to decrease by 17% and void content to rise by 33%. This phenomenon was identified to be a result of the outflow restriction increasing the number of voids trapped within the laminate and indicates that for laminates cured at low pressures resin outflow is the dominant mechanism for void reduction.

Nuclear Organization in the Spinal Cord Depends on Motor Neuron Lamination Orchestrated by Catenin and Afadin Function

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Carola Dewitz

2018-02-01

Full Text Available Motor neurons in the spinal cord are found grouped in nuclear structures termed pools, whose position is precisely orchestrated during development. Despite the emerging role of pool organization in the assembly of spinal circuits, little is known about the morphogenetic programs underlying the patterning of motor neuron subtypes. We applied three-dimensional analysis of motor neuron position to reveal the roles and contributions of cell adhesive function by inactivating N-cadherin, catenin, and afadin signaling. Our findings reveal that nuclear organization of motor neurons is dependent on inside-out positioning, orchestrated by N-cadherin, catenin, and afadin activities, controlling cell body layering on the medio-lateral axis. In addition to this lamination-like program, motor neurons undergo a secondary, independent phase of organization. This process results in segregation of motor neurons along the dorso-ventral axis of the spinal cord, does not require N-cadherin or afadin activity, and can proceed even when medio-lateral positioning is perturbed.

Influence of bress laminate volume fraction on electromechanical properties of externally laminated coated conductor tapes

Energy Technology Data Exchange (ETDEWEB)

Bautista, Zhierwinjay M.; Shin, Hyung Seop [Dept. of Mechanical Design Engineering, Andong National University, Andong (Korea, Republic of); Lee, Jae Hun; Lee, Hun Ju; Moon, Seung Hyun [SuNAM Co Ltd., Anseong (Korea, Republic of)

2016-09-15

The enhancement of mechanical properties of coated conductor (CC) tapes in practical application are usually achieved by reinforcing through lamination or electroplating metal layers on either sides of the CC tape. Mechanical or electromechanical properties of the CC tapes have been largely affected by the lamination structure under various loading modes such as tension, bending or even cyclic. In this study, the influence of brass laminate volume fraction on electromechanical properties of RCE-DR processed Gadolinium-barium-copper-oxide (GdBCO) CC tapes was investigated. The samples used were composed of single-side and both-side laminate of brass layer to the Cu-stabilized CC tape and their Ic behaviors were compared to those of the Cu-stabilized CC tape without external lamination. The stress/strain dependences of Ic in laminated CC tapes under uniaxial tension were analyzed and the irreversible stress/strain limits were determined. As a result, the increase of brass laminate volume fraction initially increased the irreversible strain limit and became gradual. The corresponding irreversible stress limit, however, showed no difference even though the brass laminate volume fraction increased to 3.4. But the irreversible load limit linearly increased with the brass laminate volume fraction.

A MEMS lamination technology based on sequential multilayer electrodeposition

International Nuclear Information System (INIS)

Kim, Minsoo; Kim, Jooncheol; Herrault, Florian; Schafer, Richard; Allen, Mark G

2013-01-01

A MEMS lamination technology based on sequential multilayer electrodeposition is presented. The process comprises three main steps: (1) automated sequential electrodeposition of permalloy (Ni 80 Fe 20 ) structural and copper sacrificial layers to form multilayer structures of significant total thickness; (2) fabrication of polymeric anchor structures through the thickness of the multilayer structures and (3) selective removal of copper. The resulting structure is a set of air-insulated permalloy laminations, the separation of which is sustained by insulating polymeric anchor structures. Individual laminations have precisely controllable thicknesses ranging from 500 nm to 5 µm, and each lamination layer is electrically isolated from adjacent layers by narrow air gaps of similar scale. In addition to air, interlamination insulators based on polymers are investigated. Interlamination air gaps with very high aspect ratio (>1:100) can be filled with polyvinylalcohol and polydimethylsiloxane. The laminated structures are characterized using scanning electron microscopy and atomic force microscopy to directly examine properties such as the roughness and the thickness uniformity of the layers. In addition, the quality of the electrical insulation between the laminations is evaluated by quantifying the eddy current within the sample as a function of frequency. Fabricated laminations are comprised of uniform, smooth (surface roughness <100 nm) layers with effective electrical insulation for all layer thicknesses and insulator approaches studied. Such highly laminated structures have potential uses ranging from energy conversion to applications where composite materials with highly anisotropic mechanical or thermal properties are required. (paper)

Analysis on and Optimization of a Circular Piezoelectric Composite Laminate for a Micro-Pump Driver

International Nuclear Information System (INIS)

Jia, Jianyuan; Wang, Weidong; Huang, Xinbo

2002-01-01

Among the various micro-pump actuation devices, piezoelectric composite laminate actuation has become an effective method. Due to lacking of analysis treatments, the design of this type micro-pump is in a great limitation. In this paper, an electromechanical-coupled mechanics model is established for the circle-flake micro-actuator. A kind of analysis and design method is presented that piezoelectric plate's radial strain induced by inverse piezoelectric effect is equivalently substituted with transverse stress on piezoelectric composite laminates. It is pointed out that the equivalent transverse load depends on the edge electric field distribution of parallel plate capacitor. The question has been solved that where the neutral plane in the piezoelectric composite laminates lies. Finally, an optimization design is developed on the radius ratio of piezoelectric-to-silicon plate radius by utilizing of FEA modeling

"Subclinical" laminitis in dairy cattle.

Science.gov (United States)

Vermunt, J J

1992-12-01

In dairying countries worldwide, the economic importance of lameness in cattle is now recognised. Laminitis is regarded as a major predisposing factor in lameness caused by claw disorders such as white zone lesions, sole ulcer, and heel horn erosion. The existence of subclinical laminitis was first suggested in the late 1970s by Dutch workers describing the symptoms of sole haemorrhages and yellowish-coloured, soft sole horn. In an attempt to clarify some of the confusing and often conflicting terminology, the literature on laminitis is reviewed. Disturbed haemodynamics, in particular repeated or prolonged dilation of arteriovenous anastomoses, have been implicated in the pathogenesis of both equine and bovine laminitis. Some characteristics of the vascular system of the bovine claw which may be of importance in the pathophysiology of the subclinical laminitis syndrome are therefore discussed. Clinical observations suggest that subclinical laminitis is a multifactorial disease. The different factors that are or may be involved in its aetiology vary in complexity and severity according to the management protocol of the animals. The possible involvement of subclinical laminitis in claw lesions is assessed.

Delamination Resistance Of Laminate Made With VBO MTM46/HTS Prepreg

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Czarnocki Piotr

2015-09-01

Full Text Available A laminate made with the Vacuum Bag Only (VBO prepregs can be cured out of autoclave. Because of low curing pressure such a process can result in deterioration of laminate mechanical properties. They can be significantly lower than those displayed by the autoclave cured ones. The resistance against delamination can be among the most affected. Since this property is a week point of all the laminates it was of particular interest. Delamination resistance of unidirectional laminate made from VBO MTM46/HTS(12K prepreg was in the scope of the presented research and the critical values of the Strain Energy Release Rates and the Paris-type equations corresponding to Mode I, Mode II and Mixed-Mode I/II static and cyclic loadings, respectively, were determined.

Nuclear Organization in the Spinal Cord Depends on Motor Neuron Lamination Orchestrated by Catenin and Afadin Function.

Science.gov (United States)

Dewitz, Carola; Pimpinella, Sofia; Hackel, Patrick; Akalin, Altuna; Jessell, Thomas M; Zampieri, Niccolò

2018-02-13

Motor neurons in the spinal cord are found grouped in nuclear structures termed pools, whose position is precisely orchestrated during development. Despite the emerging role of pool organization in the assembly of spinal circuits, little is known about the morphogenetic programs underlying the patterning of motor neuron subtypes. We applied three-dimensional analysis of motor neuron position to reveal the roles and contributions of cell adhesive function by inactivating N-cadherin, catenin, and afadin signaling. Our findings reveal that nuclear organization of motor neurons is dependent on inside-out positioning, orchestrated by N-cadherin, catenin, and afadin activities, controlling cell body layering on the medio-lateral axis. In addition to this lamination-like program, motor neurons undergo a secondary, independent phase of organization. This process results in segregation of motor neurons along the dorso-ventral axis of the spinal cord, does not require N-cadherin or afadin activity, and can proceed even when medio-lateral positioning is perturbed. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

Nuclear envelopathies: a complex LINC between nuclear envelope and pathology.

Science.gov (United States)

Janin, Alexandre; Bauer, Delphine; Ratti, Francesca; Millat, Gilles; Méjat, Alexandre

2017-08-30

Since the identification of the first disease causing mutation in the gene coding for emerin, a transmembrane protein of the inner nuclear membrane, hundreds of mutations and variants have been found in genes encoding for nuclear envelope components. These proteins can be part of the inner nuclear membrane (INM), such as emerin or SUN proteins, outer nuclear membrane (ONM), such as Nesprins, or the nuclear lamina, such as lamins A and C. However, they physically interact with each other to insure the nuclear envelope integrity and mediate the interactions of the nuclear envelope with both the genome, on the inner side, and the cytoskeleton, on the outer side. The core of this complex, called LINC (LInker of Nucleoskeleton to Cytoskeleton) is composed of KASH and SUN homology domain proteins. SUN proteins are INM proteins which interact with lamins by their N-terminal domain and with the KASH domain of nesprins located in the ONM by their C-terminal domain.Although most of these proteins are ubiquitously expressed, their mutations have been associated with a large number of clinically unrelated pathologies affecting specific tissues. Moreover, variants in SUN proteins have been found to modulate the severity of diseases induced by mutations in other LINC components or interactors. For these reasons, the diagnosis and the identification of the molecular explanation of "nuclear envelopathies" is currently challenging.The aim of this review is to summarize the human diseases caused by mutations in genes coding for INM proteins, nuclear lamina, and ONM proteins, and to discuss their potential physiopathological mechanisms that could explain the large spectrum of observed symptoms.

Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase.

Science.gov (United States)

Link, Jana; Paouneskou, Dimitra; Velkova, Maria; Daryabeigi, Anahita; Laos, Triin; Labella, Sara; Barroso, Consuelo; Pacheco Piñol, Sarai; Montoya, Alex; Kramer, Holger; Woglar, Alexander; Baudrimont, Antoine; Markert, Sebastian Mathias; Stigloher, Christian; Martinez-Perez, Enrique; Dammermann, Alexander; Alsheimer, Manfred; Zetka, Monique; Jantsch, Verena

2018-04-23

Meiotic chromosome movement is important for the pairwise alignment of homologous chromosomes, which is required for correct chromosome segregation. Movement is driven by cytoplasmic forces, transmitted to chromosome ends by nuclear membrane-spanning proteins. In animal cells, lamins form a prominent scaffold at the nuclear periphery, yet the role lamins play in meiotic chromosome movement is unclear. We show that chromosome movement correlates with reduced lamin association with the nuclear rim, which requires lamin phosphorylation at sites analogous to those that open lamina network crosslinks in mitosis. Failure to remodel the lamina results in delayed meiotic entry, altered chromatin organization, unpaired or interlocked chromosomes, and slowed chromosome movement. The remodeling kinases are delivered to lamins via chromosome ends coupled to the nuclear envelope, potentially enabling crosstalk between the lamina and chromosomal events. Thus, opening the lamina network plays a role in modulating contacts between chromosomes and the nuclear periphery during meiosis. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

Laser displacement sensor to monitor the layup process of composite laminate production

Science.gov (United States)

Miesen, Nick; Groves, Roger M.; Sinke, Jos; Benedictus, Rinze

2013-04-01

Several types of flaw can occur during the layup process of prepreg composite laminates. Quality control after the production process checks the end product by testing the specimens for flaws which are included during the layup process or curing process, however by then these flaws are already irreversibly embedded in the laminate. This paper demonstrates the use of a laser displacement sensor technique applied during the layup process of prepreg laminates for in-situ flaw detection, for typical flaws that can occur during the composite production process. An incorrect number of layers and fibre wrinkling are dominant flaws during the process of layup. These and other dominant flaws have been modeled to determine the requirements for an in-situ monitoring during the layup process of prepreg laminates.

Glucocorticoids and laminitis in the horse.

Science.gov (United States)

Johnson, Philip J; Slight, Simon H; Ganjam, Venkataseshu K; Kreeger, John M

2002-08-01

The administration of exogenously administered GCs and syndromes associated with GC excess are both attended by increased risk for the development of laminitis in adult horses. However, there exists substantial controversy as to whether excess GCs cause laminitis de novo. If true, the pathogenesis of laminitis arising from the effects of GC excess is probably different from that associated with diseases of the gastrointestinal tract and endotoxemia. Although a satisfactory explanation for the development of laminitis as a consequence of GC action is currently lacking, numerous possible and plausible theoretical mechanisms do exist. Veterinarians must exert caution with respect to the use of GCs in adult horses. The extent to which individual horses are predisposed to laminitis as a result of GC effect cannot be predicted based on current information. However, the administration of systemic GCs to horses that have been previously affected by laminitis should be used only with extreme caution, and should be accompanied by careful monitoring for further signs of laminitis. The risk of laminitis appears to be greater during treatment using some GCs (especially dexamethasone and triamcinalone) compared with others (prednisone and prednisolone). Whenever possible, to reduce the risk of laminitis, GCs should be administered locally. For example, the risk of GC-associated laminitis is evidently considerably reduced in horses affected with chronic obstructive pulmonary disease (COPD) if GC treatment is administered via inhalation. We have hypothesized that structural changes in the equine hoof that resemble laminitis may arise as a consequence of excess GC effect. Although these changes are not painful per se, and are not associated with inflammation, they could likely predispose affected horses to the development of bona fide laminitis for other reasons. Moreover, the gross morphological appearance of the chronically GC-affected hoof resembles that of a chronically

Formaldehyde and TVOC emission behavior of laminate flooring by structure of laminate flooring and heating condition.

Science.gov (United States)

An, Jae-Yoon; Kim, Sumin; Kim, Hyun-Joong

2011-03-15

Formaldehyde was measured with a desiccator, a 20 L chamber and the FLEC method. The formaldehyde emission rate from laminate was the highest at 32 °C using the desiccator, which then decreased with time. The formaldehyde emission using the 20 L small chamber and FLEC showed a similar tendency. There was a strong correlation between the formaldehyde and total volatile organic compounds (TVOCs) with both types of floorings using the two different methods. The formaldehyde emission rate and TVOC results were higher when tested using the FLEC method than with the 20 L small chamber method. The emission rate was affected by the joint edge length in laminate flooring. Toluene, ethylbenzene and xylene were the main VOCs emitted from laminate flooring, and there were more unidentified VOCs emitted than identified VOCs. The samples heated with a floor heating system emitted more formaldehyde than those heated using an air circulation system due to the temperature difference between the bottom panel and flooring. The TVOC emission level of the samples was higher when an air circulation system was used than when a floor heating system was used due to the high ventilation rate. Copyright © 2010 Elsevier B.V. All rights reserved.

A transparent, solvent-free laminated top electrode for perovskite solar cells

OpenAIRE

Makha, Mohammed; Fernandes, Silvia Let?cia; Jenatsch, Sandra; Offermans, Ton; Schleuniger, J?rg; Tisserant, Jean-Nicolas; V?ron, Anna C.; Hany, Roland

2016-01-01

Abstract A simple lamination process of the top electrode for perovskite solar cells is demonstrated. The laminate electrode consists of a transparent and conductive plastic/metal mesh substrate, coated with an adhesive mixture of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate), PEDOT:PSS, and sorbitol. The laminate electrode showed a high degree of transparency of 85%. Best cell performance was achieved for laminate electrodes prepared with a sorbitol concentration of ~30 wt% per mil...

Composite lamination method

Science.gov (United States)

Dickerson, G. E. (Inventor)

1977-01-01

A process was developed for preparing relatively thick composite laminate structure wherein thin layers of prepreg tapes are assembled, these thin layers are cut into strips that are partially cured, and stacked into the desired thickness with uncured prepreg disposed between each layer of strips. The formed laminate is finally cured and thereafter machined to the desired final dimensions.

Predisposing factors of laminitis in cattle.

Science.gov (United States)

Vermunt, J J; Greenough, P R

1994-01-01

Laminitis is regarded as a major predisposing factor in lameness caused by claw disorders. Despite intensive study, both by experiment and by clinical observation, knowledge of the precise aetiology and pathogenesis of bovine laminitis is still incomplete. It is often hypothesized that changes in the micro-circulation of the corum (dermis) of the bovine claw contribute significantly to the development of laminitis; arteriovenous anastomoses (AVAs) playing a crucial role. Many factors have been implicated as contributing causes of laminitis in cattle; the disease has a multifactorial aetiology. The cause of laminitis should be considered as a combination of predisposing factors leading to vascular (AVAs in particular) reactivity and inhibition of normal horn synthesis. Nutrition, disease, management and behaviour appear to be closely involved in the pathogenesis of bovine laminitis. The major factors predisposing to laminitis in cattle, as reported or suggested in the literature, are reviewed, including systemic disease, nutrition (barley grain, protein, carbohydrate and fibre), management (housing, bedding and exercise), calving, season, age, growth, genetics, conformation and behaviour.

Laminitis and the equine metabolic syndrome.

Science.gov (United States)

Johnson, Philip J; Wiedmeyer, Charles E; LaCarrubba, Alison; Ganjam, V K Seshu; Messer, Nat T

2010-08-01

Although much has been written about laminitis in the context of its association with inflammatory processes, recognition is growing that most cases of laminitis examined by veterinarians in private practice are those associated with pasture grazing, obesity, and insulin resistance (IR). The term 'endocrinopathic laminitis' has been adopted to classify the instances of laminitis in which the origin seems to be more strongly associated with an underlying endocrinopathy, such as either IR or the influence of corticosteroids. Results of a recent study suggest that obesity and IR represent the most common metabolic and endocrinopathic predispositions for laminitis in horses. IR also plays an important role in the pathogenesis of laminitis that develops when some horses or ponies are allowed to graze pastures at certain times of the year. The term equine metabolic syndrome (EMS) has been proposed as a label for horses whose clinical examination results (including both physical examination and laboratory testing) suggest heightened risk for developing laminitis as a result of underlying IR. Copyright (c) 2010 Elsevier Inc. All rights reserved.

Embedding piezoresistive pressure sensors to obtain online pressure profiles inside fiber composite laminates.

Science.gov (United States)

Moghaddam, Maryam Kahali; Breede, Arne; Brauner, Christian; Lang, Walter

2015-03-27

The production of large and complex parts using fiber composite materials is costly due to the frequent formation of voids, porosity and waste products. By embedding different types of sensors and monitoring the process in real time, the amount of wastage can be significantly reduced. This work focuses on developing a knowledge-based method to improve and ensure complete impregnation of the fibers before initiation of the resin cure. Piezoresistive and capacitive pressure sensors were embedded in fiber composite laminates to measure the real-time the pressure values inside the laminate. A change of pressure indicates resin infusion. The sensors were placed in the laminate and the resin was infused by vacuum. The embedded piezoresistive pressure sensors were able to track the vacuum pressure in the fiber composite laminate setup, as well as the arrival of the resin at the sensor. The pressure increase due to closing the resin inlet was also measured. In contrast, the capacitive type of sensor was found to be inappropriate for measuring these quantities. The following study demonstrates real-time monitoring of pressure changes inside the fiber composite laminate, which validate the use of Darcy's law in porous media to control the resin flow during infusion.

Continuous jute fibre reinforced laminated paper composite

Indian Academy of Sciences (India)

Jute fibre; laminated paper composite; plastic bag pollution. Abstract. Plastic bags create a serious environmental problem. The proposed jute fibre reinforced laminated paper composite and reinforcement-fibre free paper laminate may help to combat the war against this pollutant to certain extent. The paper laminate ...

Laminated dosimetric card

International Nuclear Information System (INIS)

Cox, F.M.; Chamberlain, J.D.; Shrader, E.F.; Shoffner, B.M.; Szalanczy, A.

1975-01-01

A laminated card with one or more apertures, each adapted to peripherally seal an encapsulated dosimeter, is formed by bonding a foraminous, code-adaptable, rigid sheet of low-Z material with a codedly transparent sheet of low-Z material in light-transmitting registry with particular code-holes of the rigid sheet. The laminated card may be coded to identify the person carrying it, and/or the location or circumstances related to its exposure to radiation. This card is particularly adapted for use in an instrument capable of evaluating a multiplicity of cards, substantially continuously. The coded identification from the card may be displayed by an appropriate machine, and if desired an evaluation may be recorded because of a ''parity checking'' system incorporated in each card, which permits ''auto-correction.'' Alternatively, where means for effecting the correction automatically are available, the operation of the machine may be interrupted to permit visual examination of a rejected card. The card of this invention is also coded for identifying the type of card with respect to its specific function, and whether or not a card is correctly positioned at any predetermined location during its sequential progress through the instrument in which it is evaluated. Dosimeters are evaluated and the card identified in one pass through the instrument. (auth)

A transparent, solvent-free laminated top electrode for perovskite solar cells.

Science.gov (United States)

Makha, Mohammed; Fernandes, Silvia Letícia; Jenatsch, Sandra; Offermans, Ton; Schleuniger, Jürg; Tisserant, Jean-Nicolas; Véron, Anna C; Hany, Roland

2016-01-01

A simple lamination process of the top electrode for perovskite solar cells is demonstrated. The laminate electrode consists of a transparent and conductive plastic/metal mesh substrate, coated with an adhesive mixture of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate), PEDOT:PSS, and sorbitol. The laminate electrode showed a high degree of transparency of 85%. Best cell performance was achieved for laminate electrodes prepared with a sorbitol concentration of ~30 wt% per milliliter PEDOT:PSS dispersion, and using a pre-annealing temperature of 120°C for 10 min before lamination. Thereby, perovskite solar cells with stabilized power conversion efficiencies of (7.6 ± 1.0)% were obtained which corresponds to 80% of the reference devices with reflective opaque gold electrodes.

A Theoretical Model for Estimation of Yield Strength of Fiber Metal Laminate

Science.gov (United States)

Bhat, Sunil; Nagesh, Suresh; Umesh, C. K.; Narayanan, S.

2017-08-01

The paper presents a theoretical model for estimation of yield strength of fiber metal laminate. Principles of elasticity and formulation of residual stress are employed to determine the stress state in metal layer of the laminate that is found to be higher than the stress applied over the laminate resulting in reduced yield strength of the laminate in comparison with that of the metal layer. The model is tested over 4A-3/2 Glare laminate comprising three thin aerospace 2014-T6 aluminum alloy layers alternately bonded adhesively with two prepregs, each prepreg built up of three uni-directional glass fiber layers laid in longitudinal and transverse directions. Laminates with prepregs of E-Glass and S-Glass fibers are investigated separately under uni-axial tension. Yield strengths of both the Glare variants are found to be less than that of aluminum alloy with use of S-Glass fiber resulting in higher laminate yield strength than with the use of E-Glass fiber. Results from finite element analysis and tensile tests conducted over the laminates substantiate the theoretical model.

Wettability of graphene-laminated micropillar structures

International Nuclear Information System (INIS)

Bong, Jihye; Seo, Keumyoung; Ju, Sanghyun; Park, Ji-Hoon; Ahn, Joung Real

2014-01-01

The wetting control of graphene is of great interest for electronic, mechanical, architectural, and bionic applications. In this study, the wettability of graphene-laminated micropillar structures was manipulated by changing the height of graphene-laminated structures and employing the trichlorosilane (HDF-S)-based self-assembly monolayer. Graphene-laminated micropillar structures with HDF-S exhibited higher hydrophobicity (contact angle of 129.5°) than pristine graphene thin film (78.8°), pristine graphene-laminated micropillar structures (97.5°), and HDF-S self-assembled graphene thin film (98.5°). Wetting states of the graphene-laminated micropillar structure with HDF-S was also examined by using a urea solution, which flowed across the surface without leaving any residues

Analysis of noncoplanar pressurized laminations in X2 steel pipes by non-linear finite element

Energy Technology Data Exchange (ETDEWEB)

Morales, Alfredo [Instituto Tecnologico de Puebla (Mexico). Dept. de Posgrado; Gonzalez, Jorge L.; Hallen, Jose M. [Instituto Politecnico Nacional (Mexico). Escuela Superior de Ingenieria Quimica e Industrias Extractivas (ESIQIE). Dept. de Ingenieria Metalurgica

2005-07-01

Hydrogen induced cracking is of great interest in the mechanical integrity assessment of sour gas pipelines. Multiple stepwise cracks with internal pressure called laminations are often observed in pipelines and their interaction and coalescence may significantly affect the residual strength of the pipes. In this work, the interacting fields of non coplanar pressurized laminations in the wall of a pipe under pressure are analyzed by non-lineal finite element, considering an isotropic hardening law and the real tensile properties of the X52 steel. The results are presented as the evolution of the stress fields in the interlaminar region as a function of the pressure inside the laminations. It is found that for two approaching stepwise laminations the critical pressure follows a hyperbolic type law, thus the effect of the lamination length is principal for greater lengths and for shorter lengths the effect is minimum. The critical pressure is defined as pressure inside the lamination that causes plastification of the interlaminar region. (author)

Self-Healing Laminate System

Science.gov (United States)

Beiermann, Brett A. (Inventor); Keller, Michael W. (Inventor); White, Scott R. (Inventor); Sottos, Nancy R. (Inventor)

2016-01-01

A laminate material may include a first flexible layer, and a self-healing composite layer in contact with the first flexible layer. The composite layer includes an elastomer matrix, a plurality of first capsules including a polymerizer, and a corresponding activator for the polymerizer. The laminate material may self-heal when subjected to a puncture or a tear.

Transmission electron microscope studies of the nuclear envelope in Caenorhabditis elegans embryos.

Science.gov (United States)

Cohen, Merav; Tzur, Yonatan B; Neufeld, Esther; Feinstein, Naomi; Delannoy, Michael R; Wilson, Katherine L; Gruenbaum, Yosef

2002-01-01

Nuclear membranes and nuclear pore complexes (NPCs) are conserved in both animals and plants. However, the lamina composition and the dimensions of NPCs vary between plants, yeast, and vertebrates. In this study, we established a protocol that preserves the structure of Caenorhabditis elegans embryonic cells for high-resolution studies with thin-section transmission electron microscopy (TEM). We show that the NPCs are bigger in C. elegans embryos than in yeast, with dimensions similar to those in higher eukaryotes. We also localized the C. elegans nuclear envelope proteins Ce-lamin and Ce-emerin by pre-embedding gold labeling immunoelectron microscopy. Both proteins are present at or near the inner nuclear membrane. A fraction of Ce-lamin, but not Ce-emerin, is present in the nuclear interior. Removing the nuclear membranes leaves both Ce-lamin and Ce-emerin associated with the chromatin. Eliminating the single lamin protein caused cell death as visualized by characteristic changes in nuclear architecture including condensation of chromatin, clustering of NPCs, membrane blebbing, and the presence of vesicles inside the nucleus. Taken together, these results show evolutionarily conserved protein localization, interactions, and functions of the C. elegans nuclear envelope.

Development of three dimensional seismic isolation device with laminated rubber bearing and rolling seal type air spring

International Nuclear Information System (INIS)

Okada, Yasuo; Suhara, Junji; Tamura, Tadashi; Ohta, Kazuya; Moro, Satoshi

2003-01-01

Three dimensional (3D) seismic isolation device has been developed to use for the base isolation system of the heavy building like a nuclear reactor building. The developed device is the 3D seismic isolation device that consists of the laminated rubber baring as a horizontal isolation device and the rolling seal type air spring as the vertical isolation device in series. In this research, the 3D seismic isolation device reduction model whose scale is 1/10 is made and the workability of the device by the horizontal and vertical dynamic load is examined. Two experiment parameters are considered. One is the case that the structure of the part that the horizontal load and the vertical load contact is pin condition and the other is the case of the roller condition. As a result of the examination, the workability of the vertical direction is confirmed when the horizontal load acts. The pressure resistant ability test for the air spring is performed by the monotonic pressurization. As the result, it is confirmed that pressure resistant ability improved by restricting the side deformation of the air spring and that the material of the existing air spring can withstand high pressure use sufficiently. As the result, it is confirmed that the developed 3D seismic isolation device is applicable to the actual plant. This study was performed under the sponsorship of the Ministry of Economy, Trade and Industry of Japan. (author)

A Novel Procedure for Prediction of Mixed Mode I/II in Fracture Toughness of Laminate Composites

Directory of Open Access Journals (Sweden)

M. Mahmood Shokrieh

2014-06-01

Full Text Available Delamination is one of the important modes of failure in laminated composite materials. In this respect, the mixed mode I/II fracture is the most major mode of delamination incidence in laminated composite. In the present research, a relation between the fracture toughness of double cantilever beam (DCB and asymmetric double cantilever beam (ADCB specimens is presented. The DCB and ADCB samples are used for measuring the mode I and mixed mode I/II fracture toughness (G of laminated composite materials, respectively. By considering the diversity of the stacking sequence of lay-ups, the test performance on all different types of lay-ups in order to measure the fracture toughness of laminated composites is a tedious, costly and time consuming task. The purpose of deriving this relation is to estimate the value of the strain energy release rate of laminated composite ADCB specimens by testing a unidirectional DCB. To develop this relationship, the geometry of DCB and ADCB specimens are considered to obtain fracture toughness of multi-directional laminate composites of ADCB samples with arbitrary ply sequence which may be used for design purposes. The procedure presented here reduces the calculation costs of the finite element modeling and its corresponding test significantly. The results obtained by this method are compared with those of experimental and numerical methods. It is shown that the fracture toughness of multi-directional lay-ups can be predicted by measuring the unidirectional ply with an error less than 10% demonstrating the accuracy of the procedure developed in the present research.

Lamins, laminopathies and disease mechanisms

Indian Academy of Sciences (India)

2011-07-08

Jul 8, 2011 ... Lamins, laminopathies and disease mechanisms: Possible role for proteasomal degradation of ... Mutations in the human lamin genes lead to highly degenerative genetic diseases that affect a number of different ... June 2018.

Energy Saving Glass Lamination via Selective Radio Frequency Heating

Energy Technology Data Exchange (ETDEWEB)

Allan, Shawn M; Baranova, Inessa; Poley, Joseph; Reis, Henrique

2012-02-27

This project focused on advancing radio-frequency (RF) lamination technology closer to commercial implementation, in order to reduce the energy intensity of glass lamination by up to 90%. Lamination comprises a wide range of products including autoglass, architectural safety and innovative design glass, transparent armor (e.g. bullet proof glass), smart glass, mirrors, and encapsulation of photovoltaics. Lamination is also the fastest growing segment of glass manufacturing, with photovoltaics, architectural needs, and an anticipated transition to laminated side windows in vehicles. The state-of-the-art for glass lamination is to use autoclaves, which apply heat and uniform gas pressure to bond the laminates over the course of 1 to 18 hours. Laminates consist of layers of glass or other materials bonded with vinyl or urethane interlayers. In autoclaving, significant heat energy is lost heating the chamber, pressurized air, glass racks, and the glass. In RF lamination, the heat is generated directly in the vinyl interlayer, causing it to heat and melt quickly, in just 1 to 10 minutes, without significantly heating the glass or the equipment. The main purpose of this project was to provide evidence that low energy, rapid RF lamination quality met the same standards as conventionally autoclaved windows. The development of concepts for laminating curved glass with RF lamination was a major goal. Other primary goals included developing a stronger understanding of the lamination product markets described above, and to refine the potential benefits of commercial implementation. The scope of the project was to complete implementation concept studies in preparation for continuation into advanced development, pilot studies, and commercial implementation. The project consisted of 6 main tasks. The first dealt with lamination with poly-vinyl butyral (PVB) interlayers, which prior work had shown difficulties in achieving good quality laminates, working with Pilkington North

Energy Saving Glass Lamination via Selective Radio Frequency Heating

Energy Technology Data Exchange (ETDEWEB)

Allan, Shawn M.

2012-02-27

This project focused on advancing radio-frequency (RF) lamination technology closer to commercial implementation, in order to reduce the energy intensity of glass lamination by up to 90%. Lamination comprises a wide range of products including autoglass, architectural safety and innovative design glass, transparent armor (e.g. bullet proof glass), smart glass, mirrors, and encapsulation of photovoltaics. Lamination is also the fastest growing segment of glass manufacturing, with photovoltaics, architectural needs, and an anticipated transition to laminated side windows in vehicles. The state-of-the-art for glass lamination is to use autoclaves, which apply heat and uniform gas pressure to bond the laminates over the course of 1 to 18 hours. Laminates consist of layers of glass or other materials bonded with vinyl or urethane interlayers. In autoclaving, significant heat energy is lost heating the chamber, pressurized air, glass racks, and the glass. In RF lamination, the heat is generated directly in the vinyl interlayer, causing it to heat and melt quickly, in just 1 to 10 minutes, without significantly heating the glass or the equipment. The main purpose of this project was to provide evidence that low energy, rapid RF lamination quality met the same standards as conventionally autoclaved windows. The development of concepts for laminating curved glass with RF lamination was a major goal. Other primary goals included developing a stronger understanding of the lamination product markets described above, and to refine the potential benefits of commercial implementation. The scope of the project was to complete implementation concept studies in preparation for continuation into advanced development, pilot studies, and commercial implementation. The project consisted of 6 main tasks. The first dealt with lamination with poly-vinyl butyral (PVB) interlayers, which prior work had shown difficulties in achieving good quality laminates, working with Pilkington North

Photovoltaic-Panel Laminator

Science.gov (United States)

Keenan, R.

1985-01-01

Two-piece unit heats and presses protective layers to form laminate. Rubber diaphragm between upper and lower vacuum chambers alternates between neutral position and one that presses against solar-cell array, supplying distributed force necessary to press layers of laminate together. Encapsulation helps to protect cells from environment and to ensure long panel life while allowing efficient generation of electricity from Sunlight.

Failure Behaviour of Aluminium/CFRP Laminates with Varying Fibre Orientation in Quasi-static Indentation Test

Science.gov (United States)

Romli, N. K.; Rejab, M. R. M.; Bachtiar, D.; Siregar, J.; Rani, M. F.; Salleh, Salwani Mohd; Merzuki, M. N. M.

2018-03-01

The response of the aluminium/carbon laminate was examined by an experimental work. The investigation on fibre metal laminate behaviour was done through an indentation test in a quasi-static loading. The hybrid laminate was fabricated by a compression moulding technique and used two types of carbon fibre orientations; plain weave and unidirectional. The plain weave orientation is dry fibre, and unidirectional orientation is prepreg type fibre. The plain weave carbon fibre and aluminium alloy 2024-0 was laminated by using thermoset epoxy while the unidirectional carbon fibre was pressed by using a hot press machine and cured under a specific temperature and pressure. A compression moulding technique was used for the FML fabrication. The aluminium sheet metal has been roughening by a metal sanding method which to improve the bonding between the fibre and metal layer. The main objective of this paper is to determine the failure response of the laminate under five variation of the crosshead speeds in the quasi-static loading. Based on the experimental data of the test, the result of 1 mm/min in the plain weave CFRP has lower loading than unidirectional fibre which the value of both was 4.11 kN and 4.69 kN, respectively.

Laminitis in a mature elk hind (Cervus elaphus)

DEFF Research Database (Denmark)

Gray, Heather E.; Card, Claire; Baptiste, Keith E.

2001-01-01

Laminitis should be considered as a differential diagnosis in elk presenting with shifting leg lameness, reluctance to move, recumbency and hoof wall ridging. Eliminating the underlying cause and corrective trimming lead to a good prognosis for recovery.......Laminitis should be considered as a differential diagnosis in elk presenting with shifting leg lameness, reluctance to move, recumbency and hoof wall ridging. Eliminating the underlying cause and corrective trimming lead to a good prognosis for recovery....

Dermal fibroblasts in Hutchinson-Gilford progeria syndrome with the lamin A G608G mutation have dysmorphic nuclei and are hypersensitive to heat stress

Directory of Open Access Journals (Sweden)

Worman Howard J

2005-06-01

Full Text Available Abstract Background Hutchinson-Gilford progeria syndrome (HGPS, OMIM 176670 is a rare sporadic disorder with an incidence of approximately 1 per 8 million live births. The phenotypic appearance consists of short stature, sculptured nose, alopecia, prominent scalp veins, small face, loss of subcutaneous fat, faint mid-facial cyanosis, and dystrophic nails. HGPS is caused by mutations in LMNA, the gene that encodes nuclear lamins A and C. The most common mutation in subjects with HGPS is a de novo single-base pair substitution, G608G (GGC>GGT, within exon 11 of LMNA. This creates an abnormal splice donor site, leading to expression of a truncated protein. Results We studied a new case of a 5 year-old girl with HGPS and found a heterozygous point mutation, G608G, in LMNA. Complementary DNA sequencing of RNA showed that this mutation resulted in the deletion of 50 amino acids in the carboxyl-terminal tail domain of prelamin A. We characterized a primary dermal fibroblast cell line derived from the subject's skin. These cells expressed the mutant protein and exhibited a normal growth rate at early passage in primary culture but showed alterations in nuclear morphology. Expression levels and overall distributions of nuclear lamins and emerin, an integral protein of the inner nuclear membrane, were not dramatically altered. Ultrastructural analysis of the nuclear envelope using electron microscopy showed that chromatin is in close association to the nuclear lamina, even in areas with abnormal nuclear envelope morphology. The fibroblasts were hypersensitive to heat shock, and demonstrated a delayed response to heat stress. Conclusion Dermal fibroblasts from a subject with HGPS expressing a mutant truncated lamin A have dysmorphic nuclei, hypersensitivity to heat shock, and delayed response to heat stress. This suggests that the mutant protein, even when expressed at low levels, causes defective cell stability, which may be responsible for phenotypic

Technical specifications for the successful fabrication of laminated seismic isolation bearings

International Nuclear Information System (INIS)

Kulak, R.F.

1992-01-01

High damping laminated elastomeric bearings are becoming one of the preferred devices for isolating large buildings and structures. IN the United States, the current reference design for the Advanced Liquid Metal Reactor uses laminated bearings for seismic isolation. These bearing are constructed from alternating layers of rubber and steel plates. They are typically designed for shear strains between 50 to 100 percent and expected to sustain two to three times these levels for beyond design basis loading considerations. The technical specifications used to procure these bearings are an important factor in assuring that the bearings that are installed under nuclear structures meet the performance requirements of the design. The key aspects of the current version of the Technical Specifications are discussed in this paper

A Novel Electro-Thermal Laminated Ceramic with Carbon-Based Layer

Directory of Open Access Journals (Sweden)

Yi Ji

2017-06-01

Full Text Available A novel electro-thermal laminated ceramic composed of ceramic tile, carbon-based layer, dielectric layer, and foaming ceramic layer was designed and prepared by tape casting. The surface temperature achieved at an applied voltage of 10 V by the laminated ceramics was 40.3 °C when the thickness of carbon-based suspension was 1.0 mm and the adhesive strength between ceramic tile and carbon-based layer was 1.02 ± 0.06 MPa. In addition, the thermal aging results at 100 °C up to 192 h confirmed the high thermal stability and reliability of the electro-thermal laminated ceramics. The development of this laminated ceramic with excellent electro-thermal properties and safety provides a new individual heating device which is highly expected to be widely applied in the field of indoor heat supply.

Assessment of horse owners' ability to recognise equine laminitis: A cross-sectional study of 93 veterinary diagnosed cases in Great Britain.

Science.gov (United States)

Pollard, D; Wylie, C E; Verheyen, K L P; Newton, J R

2017-11-01

Use of owner-reported data could further epidemiological knowledge of equine laminitis. However, owner recognition of laminitis has not previously been assessed. The primary objective was to establish whether cases of owner-suspected laminitis would be confirmed as laminitis by the attending veterinary surgeon. Secondary objectives were to compare owner- and veterinary-reported information from veterinary-confirmed cases of equine laminitis. Cross-sectional study. Twenty-five British veterinary practices were invited to submit laminitis reporting forms (LRFs) for active laminitis cases attended between January 2014 and October 2015; detailing 27 clinical signs, 5 underlying conditions and 7 risk factors associated with laminitis. Owners were invited to independently complete a modified LRF if reason for the veterinary visit was suspicion of laminitis. Differences between paired veterinary and owner LRFs, and between cases where owners did and did not recognise laminitis, were assessed using McNemar's and Fisher's Exact tests, respectively. Veterinary LRFs were received for 93 veterinary-diagnosed laminitis cases. All 51 owner-suspected cases were confirmed by veterinary diagnosis, with the remaining 42 (45.2%) not recognised as laminitis by owners. Undefined lameness, foot abscesses, colic and stiffness were common reasons for owner-requested veterinary visits in owner-unrecognised cases. 'Divergent growth rings' (prevalence difference: +27.3%, P = 0.01) and 'breed type' (prevalence difference: +21.2%, P = 0.04) were more commonly reported by veterinary surgeons in owner-recognised compared to owner-unrecognised cases. 'Difficulty turning', 'shifting weight' and risk factor 'body condition' were more frequently reported by veterinary surgeons whilst 'increased hoof temperature' was reported more frequently by owners. The limited clinical data restricted statistical inferences regarding the secondary objectives. All owner-suspected laminitis cases were confirmed

BILAM: a composite laminate failure-analysis code using bilinear stress-strain approximations

Energy Technology Data Exchange (ETDEWEB)

McLaughlin, P.V. Jr.; Dasgupta, A.; Chun, Y.W.

1980-10-01

The BILAM code which uses constant strain laminate analysis to generate in-plane load/deformation or stress/strain history of composite laminates to the point of laminate failure is described. The program uses bilinear stress-strain curves to model layer stress-strain behavior. Composite laminates are used for flywheels. The use of this computer code will help to develop data on the behavior of fiber composite materials which can be used by flywheel designers. In this program the stress-strain curves are modelled by assuming linear response in axial tension while using bilinear approximations (2 linear segments) for stress-strain response to axial compressive, transverse tensile, transverse compressive and axial shear loadings. It should be noted that the program attempts to empirically simulate the effects of the phenomena which cause nonlinear stress-strain behavior, instead of mathematically modelling the micromechanics involved. This code, therefore, performs a bilinear laminate analysis, and, in conjunction with several user-defined failure interaction criteria, is designed to provide sequential information on all layer failures up to and including the first fiber failure. The modus operandi is described. Code BILAM can be used to: predict the load-deformation/stress-strain behavior of a composite laminate subjected to a given combination of in-plane loads, and make analytical predictions of laminate strength.

Fatigue and fracture of fibre metal laminates

CERN Document Server

Alderliesten, René

2017-01-01

This book contributes to the field of hybrid technology, describing the current state of knowledge concerning the hybrid material concept of laminated metallic and composite sheets for primary aeronautical structural applications. It is the only book to date on fatigue and fracture of fibre metal laminates (FMLs). The first section of the book provides a general background of the FML technology, highlighting the major FML types developed and studied over the past decades in conjunction with an overview of industrial developments based on filed patents. In turn, the second section discusses the mechanical response to quasi-static loading, together with the fracture phenomena during quasi-static and cyclic loading. To consider the durability aspects related to strength justification and certification of primary aircraft structures, the third section discusses thermal aspects related to FMLs and their mechanical response to various environmental and acoustic conditions.

Modeling Bistable Composite Laminates for Piezoelectric Morphing Structures

OpenAIRE

Darryl V. Murray; Oliver J. Myers

2013-01-01

A sequential modeling effort for bistable composite laminates for piezoelectric morphing structures is presented. Thin unsymmetric carbon fiber composite laminates are examined for use of morphing structures using piezoelectric actuation. When cooling from the elevated cure temperature to room temperature, these unsymmetric composite laminates will deform. These postcure room temperature deformation shapes can be used as morphing structures. Applying a force to these deformed laminates will c...

A Mixed-Mode (I-II) Fracture Criterion for AS4/8552 Carbon/Epoxy Composite Laminate

Science.gov (United States)

Karnati, Sidharth Reddy

A majority of aerospace structures are subjected to bending and stretching loads that introduce peel and shear stresses between the plies of a composite laminate. These two stress components cause a combination of mode I and II fracture modes in the matrix layer of the composite laminate. The most common failure mode in laminated composites is delamination that affects the structural integrity of composite structures. Damage tolerant designs of structures require two types of materials data: mixed-mode (I-II) delamination fracture toughness that predicts failure and delamination growth rate that predicts the life of the structural component. This research focuses determining mixed-mode (I-II) fracture toughness under a combination of mode I and mode II stress states and then a fracture criterion for AS4/8552 composite laminate, which is widely used in general aviation. The AS4/8552 prepreg was supplied by Hexcel Corporation and autoclave fabricated into a 20-ply unidirectional laminate with an artificial delamination by a Fluorinated Ethylene Propylene (FEP) film at the mid-plane. Standard split beam specimens were prepared and tested in double cantilever beam (DCB) and end notched flexure modes to determine mode I (GIC) and II (GIIC) fracture toughnesses, respectively. The DCB specimens were also tested in a modified mixed-mode bending apparatus at GIIm /GT ratios of 0.18, 0.37, 0.57 and 0.78, where GT is total and GIIm is the mode II component of energy release rates. The measured fracture toughness, GC, was found to follow the locus a power law equation. The equation was validated for the present and literature experimental data.

Control of nuclear β-dystroglycan content is crucial for the maintenance of nuclear envelope integrity and function.

Science.gov (United States)

Vélez-Aguilera, Griselda; de Dios Gómez-López, Juan; Jiménez-Gutiérrez, Guadalupe E; Vásquez-Limeta, Alejandra; Laredo-Cisneros, Marco S; Gómez, Pablo; Winder, Steve J; Cisneros, Bulmaro

2018-02-01

β-Dystroglycan (β-DG) is a plasma membrane protein that has ability to target to the nuclear envelope (NE) to maintain nuclear architecture. Nevertheless, mechanisms controlling β-DG nuclear localization and the physiological consequences of a failure of trafficking are largely unknown. We show that β-DG has a nuclear export pathway in myoblasts that depends on the recognition of a nuclear export signal located in its transmembrane domain, by CRM1. Remarkably, NES mutations forced β-DG nuclear accumulation resulting in mislocalization and decreased levels of emerin and lamin B1 and disruption of various nuclear processes in which emerin (centrosome-nucleus linkage and β-catenin transcriptional activity) and lamin B1 (cell cycle progression and nucleoli structure) are critically involved. In addition to nuclear export, the lifespan of nuclear β-DG is restricted by its nuclear proteasomal degradation. Collectively our data show that control of nuclear β-DG content by the combination of CRM1 nuclear export and nuclear proteasome pathways is physiologically relevant to preserve proper NE structure and activity. Copyright © 2017 Elsevier B.V. All rights reserved.

Quantification of the spatial organization of the nuclear lamina as a tool for cell classification

NARCIS (Netherlands)

Righolt, C.H.; Zatreanu, D.A.; Raz, V.

2013-01-01

The nuclear lamina is the structural scaffold of the nuclear envelope that plays multiple regulatory roles in chromatin organization and gene expression as well as a structural role in nuclear stability. The lamina proteins, also referred to as lamins, determine nuclear lamina organization and

Delamination behaviour in differently copper laminated REBCO coated conductor tapes under transverse loading

Energy Technology Data Exchange (ETDEWEB)

Gorospe, Alking [Department of Mechanical Design Engineering, Andong National University, 1375 Kyungdong-Ro, Andong 760-749 (Korea, Republic of); Department of Engineering, Aurora State College of Technology, Baler Aurora 3200 (Philippines); Nisay, Arman [Department of Mechanical Design Engineering, Andong National University, 1375 Kyungdong-Ro, Andong 760-749 (Korea, Republic of); Shin, Hyung-Seop, E-mail: hsshin@andong.ac.kr [Department of Mechanical Design Engineering, Andong National University, 1375 Kyungdong-Ro, Andong 760-749 (Korea, Republic of)

2014-09-15

Highlights: • I{sub c} degradation behavior under transverse tension loading in different CC tape structure. • Weibull distribution analysis applied on delamination mechanism of CC tape. • Delamination mechanism on CC tapes depending on copper lamination type. • SEM and WDS mapping analysis of delamination sites under transverse loading. - Abstract: Laminated HTS coated conductor (CC) tapes having a unique multi-layer structure made them vulnerable when exposed to transverse loading. Electromechanical transport properties of these CC tapes can be affected by excessive transverse stresses. Due to the coefficient of thermal expansion (CTE) mismatch and incompatibility among constituent materials used in coil applications, delamination among layers occurs and causes critical current, I{sub c} degradation in the CC tapes. In this study, the delamination behaviors in copper (Cu) solder-laminated CC tapes by soldering and surround Cu-stabilized ones by electroplating under transverse tension loading were investigated. Similarly to the surround Cu-stabilized CC tapes in our previous reports, the Cu solder-laminated CC tapes also showed an abrupt and gradual I{sub c} degradation behavior. However, the Cu solder-laminated CC tapes showed different delamination morphologies as compared to the surround Cu-stabilized CC tapes; the superconducting side and the substrate side of the Cu solder laminated CC tapes were totally separated by delamination. On the other hand, the brass laminate did not show any significant effect on the delamination strength when it is added upon the surround Cu-stabilized CC tapes.

Subclinical laminitis in dairy heifers.

Science.gov (United States)

Bradley, H K; Shannon, D; Neilson, D R

1989-08-19

By causing poorer horn quality, subclinical laminitis is considered to be a major predisposing cause of other hoof problems, particularly sole ulcers in newly calved heifers. In this study the hind hooves of 136 female Friesian/Holstein cattle aged between four months and two years were examined to discover at what age the signs of subclinical laminitis appeared. Sole haemorrhages were found in the hoof horn of calves as young as five months. The consistent finding of these lesions in heifers of all ages indicated that subclinical laminitis of varying degree was a common condition during the early growing period of young dairy heifers.

Properties of Chitosan-Laminated Collagen Film

Directory of Open Access Journals (Sweden)

Vera Lazić

2012-01-01

Full Text Available The objective of this study is to determine physical, mechanical and barrier properties of chitosan-laminated collagen film. Commercial collagen film, which is used for making collagen casings for dry fermented sausage production, was laminated with chitosan film layer in order to improve the collagen film barrier properties. Different volumes of oregano essential oil per 100 mL of filmogenic solution were added to chitosan film layer: 0, 0.2, 0.4, 0.6 and 0.8 mL to optimize water vapour barrier properties. Chitosan layer with 0.6 or 0.8 % of oregano essential oil lowered the water vapour transmission rate to (1.85±0.10·10–6 and (1.78±0.03·10–6 g/(m2·s·Pa respectively, compared to collagen film ((2.51±0.05·10–6 g/(m2·s·Pa. However, chitosan-laminated collagen film did not show improved mechanical properties compared to the collagen one. Tensile strength decreased from (54.0±3.8 MPa of the uncoated collagen film to (36.3±4.0 MPa when the film was laminated with 0.8 % oregano essential oil chitosan layer. Elongation at break values of laminated films did not differ from those of collagen film ((18.4±2.7 %. Oxygen barrier properties were considerably improved by lamination. Oxygen permeability of collagen film was (1806.8±628.0·10–14 cm3/(m·s·Pa and values of laminated films were below 35·10–14 cm3/(m·s·Pa. Regarding film appearance and colour, lamination with chitosan reduced lightness (L and yellowness (+b of collagen film, while film redness (+a increased. These changes were not visible to the naked eye.

Embedded adhesive connection for laminated glass plates

DEFF Research Database (Denmark)

Hansen, Jens Zangenberg; Poulsen, S.H.; Bagger, A.

2012-01-01

The structural behavior of a new connection design, the embedded adhesive connection, used for laminated glass plates is investigated. The connection consists of an aluminum plate encapsulated in-between two adjacent triple layered laminated glass plates. Fastening between glass and aluminum...... usage in a design situation. The embedded connection shows promising potential as a future fastening system for load-carrying laminated glass plates....

Decreased nuclear stiffness via FAK-ERK1/2 signaling is necessary for osteopontin-promoted migration of bone marrow-derived mesenchymal stem cells

Energy Technology Data Exchange (ETDEWEB)

Liu, Lingling, E-mail: liulingling2012@163.com [Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Luo, Qing, E-mail: qing.luo@cqu.edu.cn [Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Sun, Jinghui, E-mail: sunjhemail@163.com [Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Wang, Aoli, E-mail: leaf13332@163.com [Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Shi, Yisong, E-mail: shiyis@cqu.edu.cn [Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Ju, Yang, E-mail: ju@mech.nagoya-u.ac.jp [Department of Mechanical Science and Engineering, Nagoya University, Nagoya 464-8603 (Japan); Morita, Yasuyuki, E-mail: morita@mech.nagoya-u.ac.jp [Department of Mechanical Science and Engineering, Nagoya University, Nagoya 464-8603 (Japan); Song, Guanbin, E-mail: song@cqu.edu.cn [Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China)

2017-06-15

Migration of bone marrow-derived mesenchymal stem cells (BMSCs) plays an important role in many physiological and pathological settings, including wound healing. During the migration of BMSCs through interstitial tissues, the movement of the nucleus must be coordinated with the cytoskeletal dynamics, which in turn affects the cell migration efficiency. Our previous study indicated that osteopontin (OPN) significantly promotes the migration of rat BMSCs. However, the nuclear behaviors and involved molecular mechanisms in OPN-mediated BMSC migration are largely unclear. In the present study, using an atomic force microscope (AFM), we found that OPN could decrease the nuclear stiffness of BMSCs and reduce the expression of lamin A/C, which is the main determinant of nuclear stiffness. Increased lamin A/C expression attenuates BMSC migration by increasing nuclear stiffness. Decreased lamin A/C expression promotes BMSC migration by decreasing nuclear stiffness. Furthermore, OPN promotes BMSC migration by diminishing lamin A/C expression and decreasing nuclear stiffness via the FAK-ERK1/2 signaling pathway. This study provides strong evidence for the role of nuclear mechanics in BMSC migration as well as new insight into the molecular mechanisms of OPN-promoted BMSC migration. - Highlights: • OPN promotes BMSC migration by decreasing nuclear stiffness. • Lamin A/C knockdown decreases, while its overexpression enhances, the nuclear stiffness of BMSCs. • Lamin A/C overexpression and downregulation affect the migration of BMSCs. • OPN diminishes lamin A/C expression and decreases nuclear stiffness through the activation of the FAK-ERK1/2 signaling pathway. • OPN promotes BMSC migration via the FAK-ERK1/2 signaling pathway.

Decreased nuclear stiffness via FAK-ERK1/2 signaling is necessary for osteopontin-promoted migration of bone marrow-derived mesenchymal stem cells

International Nuclear Information System (INIS)

Liu, Lingling; Luo, Qing; Sun, Jinghui; Wang, Aoli; Shi, Yisong; Ju, Yang; Morita, Yasuyuki; Song, Guanbin

2017-01-01

Migration of bone marrow-derived mesenchymal stem cells (BMSCs) plays an important role in many physiological and pathological settings, including wound healing. During the migration of BMSCs through interstitial tissues, the movement of the nucleus must be coordinated with the cytoskeletal dynamics, which in turn affects the cell migration efficiency. Our previous study indicated that osteopontin (OPN) significantly promotes the migration of rat BMSCs. However, the nuclear behaviors and involved molecular mechanisms in OPN-mediated BMSC migration are largely unclear. In the present study, using an atomic force microscope (AFM), we found that OPN could decrease the nuclear stiffness of BMSCs and reduce the expression of lamin A/C, which is the main determinant of nuclear stiffness. Increased lamin A/C expression attenuates BMSC migration by increasing nuclear stiffness. Decreased lamin A/C expression promotes BMSC migration by decreasing nuclear stiffness. Furthermore, OPN promotes BMSC migration by diminishing lamin A/C expression and decreasing nuclear stiffness via the FAK-ERK1/2 signaling pathway. This study provides strong evidence for the role of nuclear mechanics in BMSC migration as well as new insight into the molecular mechanisms of OPN-promoted BMSC migration. - Highlights: • OPN promotes BMSC migration by decreasing nuclear stiffness. • Lamin A/C knockdown decreases, while its overexpression enhances, the nuclear stiffness of BMSCs. • Lamin A/C overexpression and downregulation affect the migration of BMSCs. • OPN diminishes lamin A/C expression and decreases nuclear stiffness through the activation of the FAK-ERK1/2 signaling pathway. • OPN promotes BMSC migration via the FAK-ERK1/2 signaling pathway.

A novel lamin A/C mutation in a Dutch family with premature atherosclerosis.

Science.gov (United States)

Weterings, A A W; van Rijsingen, I A W; Plomp, A S; Zwinderman, A H; Lekanne Deprez, R H; Mannens, M M; van den Bergh Weerman, M A; van der Wal, A C; Pinto-Sietsma, S J

2013-07-01

We report a novel lamin A/C (LMNA) mutation, p.Glu223Lys, in a family with extensive atherosclerosis, diabetes mellitus and steatosis hepatis. Sequence analysis of LMNA (using Alamut version 2.2), co-segregation analysis, electron microscopy, extensive phenotypic evaluation of the mutation carriers and literature comparison were used to determine the loss of function of this mutation. The father of three siblings died at the age of 45 years. The three siblings and the brother and sister of the father were referred to the cardiovascular genetics department, because of the premature atherosclerosis and dysmorphic characteristics observed in the father at autopsy. The novel LMNA mutation, p.Glu223Lys, was identified in the proband and his two sons. Clinical evaluation revealed atherosclerosis, insulin resistance and hypertension in the proband and dyslipidemia and hepatic steatosis in all the patients with the mutation. Based on the facts that in silico analysis predicts a possibly pathogenic mutation, the mutation co-segregates with the disease, only fibroblasts from mutation carriers show nuclear blebbing and a similar phenotype was reported to be due to missense mutations in LMNA we conclude that we deal with a pathogenic mutation. We conclude that the phenotype is similar to Dunnigan-type familial partial lipodystrophy. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Thermoviscoelastic characterization and prediction of Kevlar/epoxy composite laminates

Science.gov (United States)

Gramoll, K. C.; Dillard, D. A.; Brinson, H. F.

1990-01-01

The thermoviscoelastic characterization of Kevlar 49/Fiberite 7714A epoxy composite lamina and the development of a numerical procedure to predict the viscoelastic response of any general laminate constructed from the same material were studied. The four orthotropic material properties, S sub 11, S sub 12, S sub 22, and S sub 66, were characterized by 20 minute static creep tests on unidirectional (0) sub 8, (10) sub 8, and (90) sub 16 lamina specimens. The Time-Temperature Superposition-Principle (TTSP) was used successfully to accelerate the characterization process. A nonlinear constitutive model was developed to describe the stress dependent viscoelastic response for each of the material properties. A numerical procedure to predict long term laminate properties from lamina properties (obtained experimentally) was developed. Numerical instabilities and time constraints associated with viscoelastic numerical techniques were discussed and solved. The numerical procedure was incorporated into a user friendly microcomputer program called Viscoelastic Composite Analysis Program (VCAP), which is available for IBM PC type computers. The program was designed for ease of use. The final phase involved testing actual laminates constructed from the characterized material, Kevlar/epoxy, at various temperatures and load level for 4 to 5 weeks. These results were compared with the VCAP program predictions to verify the testing procedure and to check the numerical procedure used in the program. The actual tests and predictions agreed for all test cases which included 1, 2, 3, and 4 fiber direction laminates.

Simulation of delamination crack growth in composite laminates: application of local and non-local interface damage models

International Nuclear Information System (INIS)

Ijaz, H.; Asad, M.

2015-01-01

The use of composite laminates is increasing in these days due to higher strength and low density values in comparison of metals. Delamination is a major source of failure in composite laminates. Damage mechanics based theories are employed to simulate the delamination phenomena between composite laminates. These damage models are inherently local and can cause the concentration of stresses around the crack tip. In the present study integral type non-local damage formulation is proposed to avoid the localization problem associated to damage formulation. A comprehensive study is carried out for the models and classical local damage model are performed and results are compared with available experimental data for un IMS/924 Carbon/fiber epoxy composite laminate. (author)

Lamin A/C mutations with lipodystrophy, cardiac abnormalities, and muscular dystrophy

NARCIS (Netherlands)

van der Kooi, A. J.; Bonne, G.; Eymard, B.; Duboc, D.; Talim, B.; van der Valk, M.; Reiss, P.; Richard, P.; Demay, L.; Merlini, L.; Schwartz, K.; Busch, H. F. M.; de Visser, M.

2002-01-01

Mutations in the lamin A/C gene are found in Emery-Dreifuss muscular dystrophy, limb girdle muscular dystrophy with cardiac conduction disturbances, dilated cardiomyopathy with conduction system disease, and familial partial lipodystrophy. Cases with lamin A/C mutations presenting with lipodystrophy

Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage

DEFF Research Database (Denmark)

Gibbs-Seymour, Ian; Markiewicz, Ewa; Bekker-Jensen, Simon

2015-01-01

Lamins A/C have been implicated in DNA damage response pathways. We show that the DNA repair protein 53BP1 is a lamin A/C binding protein. In undamaged human dermal fibroblasts (HDF), 53BP1 is a nucleoskeleton protein. 53BP1 binds to lamins A/C via its Tudor domain, and this is abrogated by DNA...... damage. Lamins A/C regulate 53BP1 levels and consequently lamin A/C-null HDF display a 53BP1 null-like phenotype. Our data favour a model in which lamins A/C maintain a nucleoplasmic pool of 53BP1 in order to facilitate its rapid recruitment to sites of DNA damage and could explain why an absence...

Evaluation of Behaviours of Laminated Glass

Science.gov (United States)

Sable, L.; Japins, G.; Kalnins, K.

2015-11-01

Visual appearance of building facades and other load bearing structures, which now are part of modern architecture, is the reason why it is important to investigate in more detail the reliability of laminated glass for civil structures. Laminated glass in particular has become one of the trendy materials, for example Apple© stores have both load carrying capacity and transparent appearance. Glass has high mechanical strength and relatively medium density, however, the risk of sudden brittle failure like concrete or other ceramics determine relatively high conservatism in design practice of glass structures. This should be changed as consumer requirements evolve calling for a safe and reliable design methodology and corresponding building standards. A design methodology for glass and glass laminates should be urgently developed and included as a chapter in Eurocode. This paper presents initial experimental investigation of behaviour of simple glass sheets and laminated glass samples in 4-point bending test. The aim of the current research is to investigate laminated glass characteristic values and to verify the obtained experimental results with finite element method for glass and EVA material in line with future European Structural Design of Glass Components code.

Strength and fatigue life evaluation of composite laminate with embedded sensors

Science.gov (United States)

Rathod, Vivek T.; Hiremath, S. R.; Roy Mahapatra, D.

2014-04-01

Prognosis regarding durability of composite structures using various Structural Health Monitoring (SHM) techniques is an important and challenging topic of research. Ultrasonic SHM systems with embedded transducers have potential application here due to their instant monitoring capability, compact packaging potential toward unobtrusiveness and noninvasiveness as compared to non-contact ultrasonic and eddy current techniques which require disassembly of the structure. However, embedded sensors pose a risk to the structure by acting as a flaw thereby reducing life. The present paper focuses on the determination of strength and fatigue life of the composite laminate with embedded film sensors like CNT nanocomposite, PVDF thin films and piezoceramic films. First, the techniques of embedding these sensors in composite laminates is described followed by the determination of static strength and fatigue life at coupon level testing in Universal Testing Machine (UTM). Failure mechanisms of the composite laminate with embedded sensors are studied for static and dynamic loading cases. The coupons are monitored for loading and failure using the embedded sensors. A comparison of the performance of these three types of embedded sensors is made to study their suitability in various applications. These three types of embedded sensors cover a wide variety of applications, and prove to be viable in embedded sensor based SHM of composite structures.

β-Catenin is critical for cerebellar foliation and lamination.

Directory of Open Access Journals (Sweden)

Jing Wen

Full Text Available The cerebellum has a conserved foliation pattern and a well-organized layered structure. The process of foliation and lamination begins around birth. β-catenin is a downstream molecule of Wnt signaling pathway, which plays a critical role in tissue organization. Lack of β-catenin at early embryonic stages leads to either prenatal or neonatal death, therefore it has been difficult to resolve its role in cerebellar foliation and lamination. Here we used GFAP-Cre to ablate β-catenin in neuronal cells of the cerebellum after embryonic day 12.5, and found an unexpected role of β-catenin in determination of the foliation pattern. In the mutant mice, the positions of fissure formation were changed, and the meninges were improperly incorporated into fissures. At later stages, some lobules were formed by Purkinje cells remaining in deep regions of the cerebellum and the laminar structure was dramatically altered. Our results suggest that β-catenin is critical for cerebellar foliation and lamination. We also found a non cell-autonomous role of β-catenin in some developmental properties of major cerebellar cell types during specific stages.

Energy Saving Glass Lamination via Selective Radio-Frequency Heating

Energy Technology Data Exchange (ETDEWEB)

Shulman, Holly S.; Allan, Shawn M.

2009-11-11

This Inventions and Innovations program supported the technical and commercial research and development needed to elevate Ceralink's energy saving process for flat glass lamination from bench scale to a self-supporting technology with significant potential for growth. Radio-frequency heating was any un-explored option for laminating glass prior to this program. With significant commercial success through time and energy savings in the wood, paper, and plastics industries, RF heating was found to have significant promise for the energy intensive glass lamination industry. A major technical goal of the program was to demonstrate RF lamination across a wide range of laminate sizes and materials. This was successfully accomplished, dispelling many skeptics' concerns about the abilities of the technology. Ceralink laminated panels up to 2 ft x 3 ft, with four sets processed simultaneously, in a 3 minute cycle. All major categories of interlayer materials were found to work with RF lamination. In addition to laminating glass, other materials including photovoltaic silicon solar cells, light emitting diodes, metallized glass, plastics (acrylic and polycarbonate), and ceramics (alumina) were found compatible with the RF process. This opens up a wide range of commercial opportunities beyond the initially targeted automotive industry. The dramatic energy savings reported for RF lamination at the bench scale were found to be maintained through the scale up of the process. Even at 2 ft x 3 ft panel sizes, energy savings are estimated to be at least 90% compared to autoclaving or vacuum lamination. With targeted promotion through conference presentations, press releases and internet presence, RF lamination has gained significant attention, drawing large audiences at American Ceramic Society meetings. The commercialization success of the project includes the establishment of a revenue-generating business model for providing process development and demonstrations for

Discordant gene expression signatures and related phenotypic differences in lamin A- and A/C-related Hutchinson-Gilford progeria syndrome (HGPS.

Directory of Open Access Journals (Sweden)

Martina Plasilova

Full Text Available Hutchinson-Gilford progeria syndrome (HGPS is a genetic disorder displaying features reminiscent of premature senescence caused by germline mutations in the LMNA gene encoding lamin A and C, essential components of the nuclear lamina. By studying a family with homozygous LMNA mutation (K542N, we showed that HGPS can also be caused by mutations affecting both isoforms, lamin A and C. Here, we aimed to elucidate the molecular mechanisms underlying the pathogenesis in both, lamin A- (sporadic and lamin A and C-related (hereditary HGPS. For this, we performed detailed molecular studies on primary fibroblasts of hetero- and homozygous LMNA K542N mutation carriers, accompanied with clinical examinations related to the molecular findings. By assessing global gene expression we found substantial overlap in altered transcription profiles (13.7%; 90/657 in sporadic and hereditary HGPS, with 83.3% (75/90 concordant and 16.7% (15/90 discordant transcriptional changes. Among the concordant ones we observed down-regulation of TWIST2, whose inactivation in mice and humans leads to loss of subcutaneous fat and dermal appendages, and loss of expression in dermal fibroblasts and periadnexial cells from a LMNA(K542N/K542N patient further confirming its pivotal role in skin development. Among the discordant transcriptional profiles we identified two key mediators of vascular calcification and bone metabolism, ENPP1 and OPG, which offer a molecular explanation for the major phenotypic differences in vascular and bone disease in sporadic and hereditary HGPS. Finally, this study correlates reduced TWIST2 and OPG expression with increased osteocalcin levels, thereby linking altered bone remodeling to energy homeostasis in hereditary HGPS.

Amyloid domains in the cell nucleus controlled by nucleoskeletal protein lamin B1 reveal a new pathway of mercury neurotoxicity

Science.gov (United States)

Arnhold, Florian; Gührs, Karl-Heinz

2015-01-01

Mercury (Hg) is a bioaccumulating trace metal that globally circulates the atmosphere and waters in its elemental, inorganic and organic chemical forms. While Hg represents a notorious neurotoxicant, the underlying cellular pathways are insufficiently understood. We identify amyloid protein aggregation in the cell nucleus as a novel pathway of Hg-bio-interactions. By mass spectrometry of purified protein aggregates, a subset of spliceosomal components and nucleoskeletal protein lamin B1 were detected as constituent parts of an Hg-induced nuclear aggregome network. The aggregome network was located by confocal imaging of amyloid-specific antibodies and dyes to amyloid cores within splicing-speckles that additionally recruit components of the ubiquitin-proteasome system. Hg significantly enhances global proteasomal activity in the nucleus, suggesting that formation of amyloid speckles plays a role in maintenance of protein homeostasis. RNAi knock down showed that lamin B1 for its part regulates amyloid speckle formation and thus likewise participates in nuclear protein homeostasis. As the Hg-induced cascade of interactions between the nucleoskeleton and protein homeostasis reduces neuronal signalling, amyloid fibrillation in the cell nucleus is introduced as a feature of Hg-neurotoxicity that opens new avenues of future research. Similar to protein aggregation events in the cytoplasm that are controlled by the cytoskeleton, amyloid fibrillation of nuclear proteins may be driven by the nucleoskeleton. PMID:25699204

Design, fabrication, testing and delivery of a feasibility model laminated ferrite memory

Science.gov (United States)

Heckler, H. C.

1973-01-01

The effect of using multiword addressing with laminated ferrite arrays was made. Both a reduction in the number of components, and a reduction in power consumption was obtained for memory capacities between one million bits and one million words. An investigation into the effect of variations in the processing steps resulted in a number of process modifications that improved the quality of the arrays. A feasibility model laminated ferrite memory system was constructed by modifying a commercial plated wire memory system to operate with laminated ferrite arrays. To provide flexibility for the testing of the laminated ferrite memory, an exerciser has been constructed to automatically control the loading and recirculation of arbitrary size checkerboard patterns of one's and zero's and to display the patterns of stored information on a CRT screen.

Self-heating forecasting for thick laminate specimens in fatigue

Science.gov (United States)

Lahuerta, F.; Westphal, T.; Nijssen, R. P. L.

2014-12-01

Thick laminate sections can be found from the tip to the root in most common wind turbine blade designs. Obtaining accurate and reliable design data for thick laminates is subject of investigations, which include experiments on thick laminate coupons. Due to the poor thermal conductivity properties of composites and the material self-heating that occurs during the fatigue loading, high temperature gradients may appear through the laminate thickness. In the case of thick laminates in high load regimes, the core temperature might influence the mechanical properties, leading to premature failures. In the present work a method to forecast the self-heating of thick laminates in fatigue loading is presented. The mechanical loading is related with the laminate self-heating, via the cyclic strain energy and the energy loss ratio. Based on this internal volumetric heat load a thermal model is built and solved to obtain the temperature distribution in the transient state. Based on experimental measurements of the energy loss factor for 10mm thick coupons, the method is described and the resulting predictions are compared with experimental surface temperature measurements on 10 and 30mm UD thick laminate specimens.

A protective effect of the laminated layer on Echinococcus granulosus survival dependent on upregulation of host arginase.

Science.gov (United States)

Amri, Manel; Touil-Boukoffa, Chafia

2015-09-01

The role of nitric oxide (NO) in host defense against Echinococcus granulosus larvae was previously reported. However, NO production by NOS2 (inducible NO synthase) is counteracted by the expression of Arginase. In the present study, our aim is to evaluate the involvement of the laminated layer (external layer of parasitic cyst) in Arginase induction and the protoscoleces (living and infective part of the cyst) survival. Our in vitro results indicate that this cystic compound increases the Arginase activity in macrophages. Moreover, C-type lectin receptors (CLRs) with specificity for mannan and the TGF-β are implicated in this effect as shown after adding Mannan and Anti-TGFβ. Interestingly, the laminated layer increases protoscoleces survival in macrophages-parasite co-cultures. Our results indicate that the laminated layer protects E. granulosus against the NOS2 protective response through Arginase pathway, a hallmark of M2 macrophages. Copyright © 2015 Elsevier B.V. All rights reserved.

PROCESS AND APPARATUS FOR SEPARATING INDIVIDUAL PANES FROM A LAMINATED GLASS SHEET

OpenAIRE

Kübler, R.; Rist, T.; Hoetger, B.

2011-01-01

The invention relates to a process for separating at least one individual pane of predefined size and edge form from a laminated glass sheet, which has at least two laminated glass sheet panes which are arranged one above another in adjacent form and between which there is arranged a plastic film, which permanently bonds the laminated glass sheet panes to one another, wherein - a laser track channel which at least weakens the plastic structure of the plastic film is laid into the plastic film...

Abnormal nuclear morphology is independent of longevity in a zmpste24-deficient fish model of Hutchinson-Gilford progeria syndrome (HGPS).

Science.gov (United States)

Tonoyama, Yasuhiro; Shinya, Minori; Toyoda, Atsushi; Kitano, Takeshi; Oga, Atsunori; Nishimaki, Toshiyuki; Katsumura, Takafumi; Oota, Hiroki; Wan, Miles T; Yip, Bill W P; Helen, Mok O L; Chisada, Shinichi; Deguchi, Tomonori; Au, Doris W T; Naruse, Kiyoshi; Kamei, Yasuhiro; Taniguchi, Yoshihito

2018-07-01

Lamin is an intermediate protein underlying the nuclear envelope and it plays a key role in maintaining the integrity of the nucleus. A defect in the processing of its precursor by a metalloprotease, ZMPSTE24, results in the accumulation of farnesylated prelamin in the nucleus and causes various diseases, including Hutchinson-Gilford progeria syndrome (HGPS). However, the role of lamin processing is unclear in fish species. Here, we generated zmpste24-deficient medaka and evaluated their phenotype. Unlike humans and mice, homozygous mutants did not show growth defects or lifespan shortening, despite lamin precursor accumulation. Gonadosomatic indices, blood glucose levels, and regenerative capacity of fins were similar in 1-year-old mutants and their wild-type (WT) siblings. Histological examination showed that the muscles, subcutaneous fat tissues, and gonads were normal in the mutants at the age of 1 year. However, the mutants showed hypersensitivity to X-ray irradiation, although p53target genes, p21 and mdm2, were induced 6 h after irradiation. Immunostaining of primary cultured cells from caudal fins and visualization of nuclei using H2B-GFP fusion proteins revealed an abnormal nuclear shape in the mutants both in vitro and in vivo. The telomere lengths were significantly shorter in the mutants compared to WT. Taken together, these results suggest that zmpste24-deficient medaka phenocopied HGPS only partially and that abnormal nuclear morphology and lifespan shortening are two independent events in vertebrates. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

Fire-Resistant Hydrogel-Fabric Laminates: A Simple Concept That May Save Lives.

Science.gov (United States)

Illeperuma, Widusha R K; Rothemund, Philipp; Suo, Zhigang; Vlassak, Joost J

2016-01-27

There is a large demand for fabrics that can survive high-temperature fires for an extended period of time, and protect the skin from burn injuries. Even though fire-resistant polymer fabrics are commercially available, many of these fabrics are expensive, decompose rapidly, and/or become very hot when exposed to high temperatures. We have developed a new class of fire-retarding materials by laminating a hydrogel and a fabric. The hydrogel contains around 90% water, which has a large heat capacity and enthalpy of vaporization. When the laminate is exposed to fire, a large amount of energy is absorbed as water heats up and evaporates. The temperature of the hydrogel cannot exceed 100 °C until it is fully dehydrated. The fabric has a low thermal conductivity and maintains the temperature gradient between the hydrogel and the skin. The laminates are fabricated using a recently developed tough hydrogel to ensure integrity of the laminate during processing and use. A thermal model predicts the performance of the laminates and shows that they have excellent heat resistance in good agreement with experiments, making them viable candidates in life saving applications such as fire-resistant blankets or apparel.

Fatigue Analysis of Notched Laminates: A Time-Efficient Macro-Mechanical Approach

Science.gov (United States)

Naghipour, P.; Pineda, E. J.; Bednarcyk, B. A.; Arnold, S. M.; Waas, A. M.

2016-01-01

A coupled transversely isotropic deformation and damage fatigue model is implemented within the finite element method and was utilized along with a static progressive damage model to predict the fatigue life, stiffness degradation as a function of number of cycles, and post-fatigue tension and compression response of notched, multidirectional laminates. Initially, the material parameters for the fatigue model were obtained utilizing micromechanics simulations and the provided [0], [90] and [plus or minus 45] experimental composite laminate S-N (stress-cycle) data. Within the fatigue damage model, the transverse and shear properties of the plies were degraded with an isotropic scalar damage variable. The damage in the longitudinal (fiber) ply direction was suppressed, and only the strength of the fiber was degraded as a function of fatigue cycles. A maximum strain criterion was used to capture the failure in each element, and once this criterion was satisfied, the longitudinal stiffness of the element was decreased by a factor of 10 (sup 4). The resulting, degraded properties were then used to calculate the new stress state. This procedure was repeated until final failure of the composite laminate was achieved or a specified number of cycles reached. For post-fatigue tension and compression behavior, four internal state variables were used to control the damage and failure. The predictive capability of the above-mentioned approach was assessed by performing blind predictions of the notched multidirectional IM7/977-3 composite laminates response under fatigue and post-fatigue tensile and compressive loading, followed by a recalibration phase. Although three different multidirectional laminates were analyzed in the course of this study, only detailed results (i.e., stiffness degradation and post-fatigue stress-train curves as well as damage evolution states for a single laminate ([30/60/90/minus 30/minus 60] (sub 2s)) are discussed in detail here.

Combined tension and bending testing of tapered composite laminates

Science.gov (United States)

O'Brien, T. Kevin; Murri, Gretchen B.; Hagemeier, Rick; Rogers, Charles

1994-11-01

A simple beam element used at Bell Helicopter was incorporated in the Computational Mechanics Testbed (COMET) finite element code at the Langley Research Center (LaRC) to analyze the responce of tappered laminates typical of flexbeams in composite rotor hubs. This beam element incorporated the influence of membrane loads on the flexural response of the tapered laminate configurations modeled and tested in a combined axial tension and bending (ATB) hydraulic load frame designed and built at LaRC. The moments generated from the finite element model were used in a tapered laminated plate theory analysis to estimate axial stresses on the surface of the tapered laminates due to combined bending and tension loads. Surfaces strains were calculated and compared to surface strains measured using strain gages mounted along the laminate length. The strain distributions correlated reasonably well with the analysis. The analysis was then used to examine the surface strain distribution in a non-linear tapered laminate where a similarly good correlation was obtained. Results indicate that simple finite element beam models may be used to identify tapered laminate configurations best suited for simulating the response of a composite flexbeam in a full scale rotor hub.

Mechanical performance of laminated composites incorporated with nanofibrous membranes

International Nuclear Information System (INIS)

Liu, L.; Huang, Z.-M.; He, C.L.; Han, X.J.

2006-01-01

The effect of non-woven nanofibrous membranes as interlaminar interfaces on the mechanical performance of laminated composites was investigated experimentally. The nanofibrous membranes are porous, thin and lightweight, and exhibit toughness and strength to some extent. They give little increase in weight and thickness when incorporated into a laminate. More important, they can be used as a functional agent carrier for the laminate. The nanofiber membranes used in this paper were prepared by electrospinning of Nylon-6 (PA6), Epoxy 609 (EPO 1691-410) and thermoplastic polyurethane (TPU), with a thickness ranging from 20 to 150 μm. The non-woven fabrics were attached to one side of a glass/epoxy fabric lamina prior to lamination and each fabric was arranged in between two adjacent plies of the laminate. The nanofibrous membranes were characterized through scanning electron microscopy (SEM) and tensile testing, whereas the mechanical properties of the laminate were understood in terms of three-point bending and short-beam shear tests. Results have shown that the nanofibrous membranes in the ply interfaces with a proper thickness did not affect the mechanical performance of the composite laminates significantly

Complex phenotype linked to a mutation in exon 11 of the lamin A/C gene: Hypertrophic cardiomyopathy, atrioventricular block, severe dyslipidemia and diabetes.

Science.gov (United States)

Francisco, Ana Rita G; Santos Gonçalves, Inês; Veiga, Fátima; Mendes Pedro, Mónica; Pinto, Fausto J; Brito, Dulce

2017-09-01

The lamin A/C (LMNA) gene encodes lamins A and C, which have an important role in nuclear cohesion and chromatin organization. Mutations in this gene usually lead to the so-called laminopathies, the primary cardiac manifestations of which are dilated cardiomyopathy and intracardiac conduction defects. Some mutations, associated with lipodystrophy but not cardiomyopathy, have been linked to metabolic abnormalities such as diabetes and severe dyslipidemia. Herein we describe a new phenotype associated with a mutation in exon 11 of the LMNA gene: hypertrophic cardiomyopathy, atrioventricular block, severe dyslipidemia and diabetes. A 64-year-old woman with hypertrophic cardiomyopathy and a point mutation in exon 11 of the LMNA gene (c.1718C>T, Ser573Leu) presented with severe symptomatic ventricular hypertrophy and left ventricular outflow tract obstruction. She underwent septal alcohol ablation, followed by Morrow myectomy. The patient was also diagnosed with severe dyslipidemia, diabetes and obesity, and fulfilled diagnostic criteria for metabolic syndrome. No other characteristics of LMNA mutation-related phenotypes were identified. The development of type III atrioventricular block with no apparent cause, and mildly depressed systolic function, prompted referral for cardiac resynchronization therapy. In conclusion, the association between LMNA mutations and different phenotypes is complex and not fully understood, and can present with a broad spectrum of severity. Copyright © 2017 Sociedade Portuguesa de Cardiologia. Publicado por Elsevier España, S.L.U. All rights reserved.

Guided wave and damage detection in composite laminates using different fiber optic sensors.

Science.gov (United States)

Li, Fucai; Murayama, Hideaki; Kageyama, Kazuro; Shirai, Takehiro

2009-01-01

Guided wave detection using different fiber optic sensors and their applications in damage detection for composite laminates were systematically investigated and compared in this paper. Two types of fiber optic sensors, namely fiber Bragg gratings (FBG) and Doppler effect-based fiber optic (FOD) sensors, were addressed and guided wave detection systems were constructed for both types. Guided waves generated by a piezoelectric transducer were propagated through a quasi-isotropic carbon fiber reinforced plastic (CFRP) laminate and acquired by these fiber optic sensors. Characteristics of these fiber optic sensors in ultrasonic guided wave detection were systematically compared. Results demonstrated that both the FBG and FOD sensors can be applied in guided wave and damage detection for the CFRP laminates. The signal-to-noise ratio (SNR) of guided wave signal captured by an FOD sensor is relatively high in comparison with that of the FBG sensor because of their different physical principles in ultrasonic detection. Further, the FOD sensor is sensitive to the damage-induced fundamental shear horizontal (SH(0)) guided wave that, however, cannot be detected by using the FBG sensor, because the FOD sensor is omnidirectional in ultrasound detection and, in contrast, the FBG sensor is severely direction dependent.

EB curable laminating adhesives

International Nuclear Information System (INIS)

Matsuyama, Asao; Kobayashi, Masahide; Gotoh, Sakiko

1992-01-01

New developed solvent free EB curable laminating adhesives have two liquid components, A with hydroxy and acryloyl group, B with isocyanate and acryloyl group in a molecule. These EB laminating adhesives do not need any aging process, which is a big advantage, and are very suitable for environment, safety, and health because of no heating process and solvent free formulas. And we have made basic research about the relation of peel strength or heat seal strength versus Tg of cured film, elongation at break, elastic modulus, and so on. Basic specifications of the new developed adhesives are shown. (author)

Behaviour of Mechanically Laminated CLT Members

Science.gov (United States)

Kuklík, P.; Velebil, L.

2015-11-01

Cross laminated timber (CLT) is one of the structural building systems based on the lamination of multiple layers, where each layer is oriented perpendicularly to each other. Recent requirements are placed to develop an alternative process based on the mechanical lamination of the layers, which is of particular interest to our research group at the University Centre for Energy Efficient Buildings. The goal is to develop and verify the behaviour of mechanically laminated CLT wall panels exposed to shear stresses in the plane. The shear resistance of mechanically jointed CLT is ensured by connecting the layers by screws. The paper deals with the experimental analysis focused on the determination of the torsional stiffness and the slip modulus of crossing areas for different numbers of orthogonally connected layers. The results of the experiments were compared with the current analytical model.

Tank type nuclear reactors

International Nuclear Information System (INIS)

Naito, Kesahiro; Shimoyashiki, Shigehiro; Yokota, Norikatsu; Takahashi, Kazuo.

1985-01-01

Purpose: To improve the seismic proofness and the radiation shielding of LMFBR type reactors by providing the reactor with a structure reduced in the size and the weight, excellent in satisfactory heat insulating property and having radioactive material capturing performance. Constitution: Two sheets of ceramic plate members (for instance, mullite, steatite, beryllium ceramics or the like) which can be fabricated into plate-like shape and have high heat insulating property are overlapped with each other, between which magnetic heat-insulating material with magnetizing magnetic ceramics (for example, Lisub(0.5)Fesub(2.5)O 4 , Ni-Fe 2 O 4 , Fe-Fe 2 O 4 ) are sandwiched and the whole assembly is covered with metal coating material (for example, stainless steels). The inside of the coating material is evacuated or filled with an inert gas with low heat-conductivity (argon) at a pressure less than 1 kg/cm 2 abs, considering that the temperature goes higher and the inner pressure increases upon operation. In this way, the size of the laminated structure can be reduced to about 1/7 of the conventional case. The magnetic heat insulating materials can capture the magnetic impurities in sodium. (Kawakami, Y.)

Risk prediction of ventricular arrhythmias and myocardial function in Lamin A/C mutation positive subjects

DEFF Research Database (Denmark)

Hasselberg, Nina E; Edvardsen, Thor; Petri, Helle

2014-01-01

Mutations in the Lamin A/C gene may cause atrioventricular block, supraventricular arrhythmias, ventricular arrhythmias (VA), and dilated cardiomyopathy. We aimed to explore the predictors and the mechanisms of VA in Lamin A/C mutation-positive subjects.METHODS AND RESULTS: We included 41 Lamin A/C...

An update on equine laminitis

OpenAIRE

Laskoski, Luciane Maria; Valadão, Carlos Augusto Araújo; Dittrich, Rosangela Locatelli; Deconto, Ivan; Faleiros, Rafael Resende

2016-01-01

ABSTRACT: Laminitis is a severe podal affection, which pathophysiology remains partially renowned. Ischemic, enzymatic, metabolic and inflammatory mechanisms are connected to the development of laminar lesions. However, few therapeutic measures are effective to prevent or control the severity of acute laminitis and its prodromal stage, which often determines serious complications such as rotation and/or sinking of the distal phalanx and even the loss of hoof. The purpose of this study is to c...

Experience of air transport of nuclear fuel material as type A package

International Nuclear Information System (INIS)

Kawasaki, Masashi; Kageyama, Tomio; Suzuki, Toru

2004-01-01

Special law on nuclear disaster countermeasures (hereafter called as to nuclear disaster countermeasures low) that is domestic law for dealing with measures for nuclear disaster, was enforced in June, 2000. Therefore, nuclear enterprise was obliged to report accidents as required by nuclear disaster countermeasures law, besides meeting the technical requirement of existent transport regulation. For overseas procurement of plutonium reference materials that are needed for material accountability, A Type package must be transported by air. Therefore, concept of air transport of nuclear fuel materials according to the nuclear disaster countermeasures law was discussed, and the manual including measures against accident in air transport was prepared for the oversea procurement. In this presentation, the concept of air transport of A Type package containing nuclear fuel materials according to the nuclear disaster countermeasures law, and the experience of a transportation of plutonium solution from France are shown. (author)

Nuclear Zpif-type plots

International Nuclear Information System (INIS)

Ma Yugang

2000-01-01

Isospin dependent classical molecular dynamics model is used to investigate the nuclear disassembly of 129 Xe. Zpif-type plot in the field of linguistics is tested for the rank-classified cluster arrangement from this nuclear disassembly. It is found that the average cluster charge (or mass) of rank n in the charge (or mass) list is exactly inverse to its rank, i.e. there exists Zpif's law at the point of the liquid gas phase transition. This novel criterion can be used to diagnose the nuclear liquid gas phase transition experimentally and theoretically

Effect of stacking angles on mechanical properties and damage propagation of plain woven carbon fiber laminates

Science.gov (United States)

Zhuang, Weimin; Ao, Wenhong

2018-03-01

Damage propagation induced failure is a predominant damage mechanism. This study is aimed at assessing the damage state and damage propagation induced failure with different stacking angles, of woven carbon fiber/epoxy laminates subjected to quasi-static tensile and bending load. Different stages of damage processing and damage behavior under the bending load are investigated by Scanning Electron Microscopy (SEM). The woven carbon fiber/epoxy laminates which are stacked at six different angles (0°, 15°, 30°, 45°, 60°, 75°) with eight plies have been analyzed: [0]8, [15]8, [30]8, [45]8, [60]8, [75]8. Three-point bending test and quasi-static tensile test are used in validating the woven carbon fiber/epoxy laminates’ mechanical properties. Furthermore, the damage propagation and failure modes observed under flexural loading is correlated with flexural force and load-displacement behaviour respectively for the laminates. The experimental results have indicated that [45]8 laminate exhibits the best flexural performance in terms of energy absorption duo to its pseudo-ductile behaviour but the tensile strength and flexural strength drastically decreased compared to [0]8 laminate. Finally, SEM micrographs of specimens and fracture surfaces are used to reveal the different types of damage of the laminates with different stacking angles.

Damage detection in multilayered fiber-metal laminates using guided-wave phased array

Energy Technology Data Exchange (ETDEWEB)

Maghsoodi, Ameneh; Ohadi, Abdolrezap; Sadighi, Mojtaba; Amindavar, Hamidreza [Amirkabir University, Tehran (Iran, Islamic Republic of)

2016-05-15

This study employs the Lamb wave method to detect damage in Fiber-metal laminates (FMLs). The method is based on quasiisotropic behavior approximation and beam forming techniques. Delay and sum and minimum variance distorsionless response beam formers are applied to a uniform linear phased array. The simulation in finite element software is conducted to evaluate the performance of the presented procedure. The two types of damage studied are the following: (1) Delamination between fiber-epoxy and metal layers and (2) crack on the metal layer. The present study has the following important contributions: (1) Health monitoring of multi-damaged FMLs using Lamb waves and beam forming technique, (2) detection of damage type, (3) detection of damage size by 1D phased array, and (4) identification of damages that occurred very close to the laminate edges or close to each other.

Mechanical properties of laminated composites of Al 1100/Al 2024 in three aging states

International Nuclear Information System (INIS)

Pessoa, C.S.

1984-01-01

Laminated composites consisting of alternating layers of Al 2024 were produced by hot rolling, with a volume fraction of 49% of Al 2024. These composites used in nuclear reactor tecnology, were given under-aging, aging and over-aging heat treatments at 448k for 1h, 4h and 96h, respectively. Tests of microhardness, tension and fatigue in the divider orientation were performed. (Author) [pt

Instantaneous mechanical fastening of quasi-isotropic CFRP laminates by a self-piercing rivet

OpenAIRE

上田, 政人; 三宅, 崇太郎; 長谷川, 寛幸; 平野, 義鎭; Ueda, Masahito; Miyake, Sotaro; Hasegawa, Hiroyuki; Hirano, Yoshiyasu

2012-01-01

A modified self-piercing rivet (SPR) has been proposed to mechanically fasten CFRP laminates. The modified SPR consists of a rivet body and two flat washers. The two flat washers were used to suppress delamination in the CFRP laminates at the point of piercing. The advantages of the modified SPR for fastening CFRP laminates are instantaneous process time and low cost. Any pretreatments such as surface treatments or hole drilling are not required. In this study, the viability of the modified S...

Experimental and Numerical Simulation Analysis of Typical Carbon Woven Fabric/Epoxy Laminates Subjected to Lightning Strike

Science.gov (United States)

Yin, J. J.; Chang, F.; Li, S. L.; Yao, X. L.; Sun, J. R.; Xiao, Y.

2017-12-01

To clarify the evolution of damage for typical carbon woven fabric/epoxy laminates exposed to lightning strike, artificial lightning testing on carbon woven fabric/epoxy laminates were conducted, damage was assessed using visual inspection and damage peeling approaches. Relationships between damage size and action integral were also elucidated. Results showed that damage appearance of carbon woven fabric/epoxy laminate presents circular distribution, and center of the circle located at the lightning attachment point approximately, there exist no damage projected area dislocations for different layers, visual damage territory represents maximum damage scope; visible damage can be categorized into five modes: resin ablation, fiber fracture and sublimation, delamination, ablation scallops and block-shaped ply-lift; delamination damage due to resin pyrolysis and internal pressure exist obvious distinguish; project area of total damage is linear with action integral for the same type specimens, that of resin ablation damage is linear with action integral, but no correlation with specimen type, for all specimens, damage depth is linear with logarithm of action integral. The coupled thermal-electrical model constructed is capable to simulate the ablation damage for carbon woven fabric/epoxy laminates exposed to simulated lightning current through experimental verification.

Minimum weight design of composite laminates for multiple loads

International Nuclear Information System (INIS)

Krikanov, A.A.; Soni, S.R.

1995-01-01

A new design method of constructing optimum weight composite laminates for multiple loads is proposed in this paper. A netting analysis approach is used to develop an optimization procedure. Three ply orientations permit development of optimum laminate design without using stress-strain relations. It is proved that stresses in minimum weight laminate reach allowable values in each ply with given load. The optimum ply thickness is defined at maximum value among tensile and compressive loads. Two examples are given to obtain optimum ply orientations, thicknesses and materials. For comparison purposes, calculations of stresses are done in orthotropic material using classical lamination theory. Based upon these calculations, matrix degrades at 30 to 50% of ultimate load. There is no fiber failure and therefore laminates withstand all applied loads in both examples

Existence region of phases of laminated perovskite-like structre of A/sub 2/B/sub 2/O/sub 7/ composition

Energy Technology Data Exchange (ETDEWEB)

Sych, A M; Titov, Yu A [Kievskij Gosudarstvennyj Univ. (Ukrainian SSR)

1982-06-01

Generalizing the known data for ferroelectrics of A/sub 2/B/sub 2/O/sub 7/ type (LnTi/sub 2/O/sub 7/, in particular) geometrical conditions of existence of laminated perovskite-like structure are determined: 0.603 A < anti Rsub(Bsup(6)) <= 0.665 A, anti Rsub(Asup(12))/Rsub(Bsup(6)) > 2.045. The geometrical conditions presented are necessary but not sufficient. A supposition is made that phases GaLnTiNbO/sub 7/ (Ln = Pr - Eu) and CaLnTiTaO/sub 7/ (Ln = La - Eu) with laminated perovskite-like structure can be prepared under high pressures.

Fatigue crack initiation in hybrid boron/glass/aluminum fiber metal laminates

International Nuclear Information System (INIS)

Chang, P.-Y.; Yeh, P.-C.; Yang, J.-M.

2008-01-01

The fatigue crack initiation behavior of a high modulus and hybrid boron/glass/aluminum fiber/metal laminate (FML) was investigated experimentally and analytically. Two types of hybrid boron/glass/aluminum FMLs were fabricated and studied, which consisted of aluminum alloy sheets as the metal layers and a mixture of boron fibers and glass fibers as the composite layers. For the first type, the boron fiber/prepreg and the glass fiber/prepreg were used separately in the composite layers, and for the second type, the boron fibers and the glass fibers were mingled together to form a hybrid boron/glass/prepreg composite layer. These hybrid FMLs were consolidated using an autoclave curing process. The incorporation of the boron fibers improved the Young's modulus of the composite layer in FMLs, which in turn, would improve the fatigue crack initiation life of the Al sheet. The experimental results clearly showed that the fatigue crack initiation lives for both types of hybrid boron/glass/aluminum FMLs were superior to the monolithic aluminum alloy under the same loading condition. An analytical approach was proposed to calculate the fatigue crack initiation lives of hybrid boron/glass/aluminum FMLs based on the classical laminate theory and the small-crack theory. A good correlation was obtained between the predictions and the experimental results

Human Cytomegalovirus Nuclear Egress Proteins Ectopically Expressed in the Heterologous Environment of Plant Cells are Strictly Targeted to the Nuclear Envelope.

Science.gov (United States)

Lamm, Christian E; Link, Katrin; Wagner, Sabrina; Milbradt, Jens; Marschall, Manfred; Sonnewald, Uwe

2016-03-10

In all eukaryotic cells, the nucleus forms a prominent cellular compartment containing the cell's nuclear genome. Although structurally similar, animal and plant nuclei differ substantially in details of their architecture. One example is the nuclear lamina, a layer of tightly interconnected filament proteins (lamins) underlying the nuclear envelope of metazoans. So far no orthologous lamin genes could be detected in plant genomes and putative lamin-like proteins are only poorly described in plants. To probe for potentially conserved features of metazoan and plant nuclear envelopes, we ectopically expressed the core nuclear egress proteins of human cytomegalovirus pUL50 and pUL53 in plant cells. pUL50 localizes to the inner envelope of metazoan nuclei and recruits the nuclear localized pUL53 to it, forming heterodimers. Upon expression in plant cells, a very similar localization pattern of both proteins could be determined. Notably, pUL50 is specifically targeted to the plant nuclear envelope in a rim-like fashion, a location to which coexpressed pUL53 becomes strictly corecruited from its initial nucleoplasmic distribution. Using pUL50 as bait in a yeast two-hybrid screening, the cytoplasmic re-initiation supporting protein RISP could be identified. Interaction of pUL50 and RISP could be confirmed by coexpression and coimmunoprecipitation in mammalian cells and by confocal laser scanning microscopy in plant cells, demonstrating partial pUL50-RISP colocalization in areas of the nuclear rim and other intracellular compartments. Thus, our study provides strong evidence for conserved structural features of plant and metazoan nuclear envelops and identifies RISP as a potential pUL50-interacting plant protein.

Disruption of PCNA-lamins A/C interactions by prelamin A induces DNA replication fork stalling.

Science.gov (United States)

Cobb, Andrew M; Murray, Thomas V; Warren, Derek T; Liu, Yiwen; Shanahan, Catherine M

2016-09-02

The accumulation of prelamin A is linked to disruption of cellular homeostasis, tissue degeneration and aging. Its expression is implicated in compromised genome stability and increased levels of DNA damage, but to date there is no complete explanation for how prelamin A exerts its toxic effects. As the nuclear lamina is important for DNA replication we wanted to investigate the relationship between prelamin A expression and DNA replication fork stability. In this study we report that the expression of prelamin A in U2OS cells induced both mono-ubiquitination of proliferating cell nuclear antigen (PCNA) and subsequent induction of Pol η, two hallmarks of DNA replication fork stalling. Immunofluorescence microscopy revealed that cells expressing prelamin A presented with high levels of colocalisation between PCNA and γH2AX, indicating collapse of stalled DNA replication forks into DNA double-strand breaks. Subsequent protein-protein interaction assays showed prelamin A interacted with PCNA and that its presence mitigated interactions between PCNA and the mature nuclear lamina. Thus, we propose that the cytotoxicity of prelamin A arises in part, from it actively competing against mature lamin A to bind PCNA and that this destabilises DNA replication to induce fork stalling which in turn contributes to genomic instability.

Optimal Design of Laminated Composite Beams

DEFF Research Database (Denmark)

Blasques, José Pedro Albergaria Amaral

model for the analysis of laminated composite beams is proposed. The structural analysis is performed in a beam finite element context. The development of a finite element based tool for the analysis of the cross section stiffness properties is described. The resulting beam finite element formulation...... is able to account for the effects of material anisotropy and inhomogeneity in the global response of the beam. Beam finite element models allow for a significant reduction in problem size and are therefore an efficient alternative in computationally intensive applications like optimization frameworks...... design of laminated composite beams. The devised framework is applied in the optimal design of laminated composite beams with different cross section geometries and subjected to different load cases. Design criteria such as beam stiffness, weight, magnitude of the natural frequencies of vibration...

Boron nitride nanoparticle enhanced prepregs: A novel route for manufacturing aerospace structural composite laminate

Energy Technology Data Exchange (ETDEWEB)

Kelkar, Ajit D., E-mail: kelkar@ncat.edu [Joint School of Nanoscience and Nanoengineering, North Carolina Agricultural and Technical State University, Greensboro, NC, 27401 (United States); Tian, Qiong [Joint School of Nanoscience and Nanoengineering, North Carolina Agricultural and Technical State University, Greensboro, NC, 27401 (United States); School of Science, Xi' an Jiaotong University, Xi' an, 710049 (China); Yu, Demei [School of Science, Xi' an Jiaotong University, Xi' an, 710049 (China); Zhang, Lifeng, E-mail: lzhang@ncat.edu [Joint School of Nanoscience and Nanoengineering, North Carolina Agricultural and Technical State University, Greensboro, NC, 27401 (United States)

2016-06-15

Boron nitride nanoparticles (BNNPs) were surface functionalized and subsequently applied to surface of fiberglass prepregs to fabricate hybrid BNNPs/fiberglass/epoxy composite laminate. A systematic and comparative study on BNNPs functionalization routes and their effects on morphology, mechanical property and thermal conductivity of final BNNPs enhanced composite laminates was performed. The functionalized BNNPs were characterized by Fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The composite laminates with surface functionalized BNNPs demonstrated improvement in tensile and flexural strength and modulus as well as in thermal conductivity compared to the composite laminate with pristine BNNPs while physically functionalized BNNPs outperformed chemically functionalized BNNPs in all cases. SEM images indicated better compatibility and dispersion of BNNPs in epoxy matrix following either of functionalization route. BNNPs bear great radiation-shielding capability. This investigation revealed a novel and industrially feasible route to incorporate BNNPs in aerospace structural materials. - Highlights: • BNNPs were surface functionalized and applied onto fiberglass prepreg. • The BNNPs enhanced prepreg was employed to make hybrid BNNPs/fiberglass/epoxy composite laminate. • The hybrid laminate presented significant improvement in mechanical strength and thermal conductivity. • This investigation revealed a novel and industrially feasible route to incorporate BNNPs in aerospace structural materials.

Boron nitride nanoparticle enhanced prepregs: A novel route for manufacturing aerospace structural composite laminate

International Nuclear Information System (INIS)

Kelkar, Ajit D.; Tian, Qiong; Yu, Demei; Zhang, Lifeng

2016-01-01

Boron nitride nanoparticles (BNNPs) were surface functionalized and subsequently applied to surface of fiberglass prepregs to fabricate hybrid BNNPs/fiberglass/epoxy composite laminate. A systematic and comparative study on BNNPs functionalization routes and their effects on morphology, mechanical property and thermal conductivity of final BNNPs enhanced composite laminates was performed. The functionalized BNNPs were characterized by Fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The composite laminates with surface functionalized BNNPs demonstrated improvement in tensile and flexural strength and modulus as well as in thermal conductivity compared to the composite laminate with pristine BNNPs while physically functionalized BNNPs outperformed chemically functionalized BNNPs in all cases. SEM images indicated better compatibility and dispersion of BNNPs in epoxy matrix following either of functionalization route. BNNPs bear great radiation-shielding capability. This investigation revealed a novel and industrially feasible route to incorporate BNNPs in aerospace structural materials. - Highlights: • BNNPs were surface functionalized and applied onto fiberglass prepreg. • The BNNPs enhanced prepreg was employed to make hybrid BNNPs/fiberglass/epoxy composite laminate. • The hybrid laminate presented significant improvement in mechanical strength and thermal conductivity. • This investigation revealed a novel and industrially feasible route to incorporate BNNPs in aerospace structural materials.

Current perpendicular to plane giant magnetoresistance in laminated nanostructures

International Nuclear Information System (INIS)

Vedyayev, A.; Zhukov, I.; Dieny, B.

2005-01-01

We theoretically studied spin-dependent electron transport perpendicular-to-plain (CPP) in magnetic laminated multilayered structures by using Kubo formalism. We took into account not only bulk scattering, but the interface resistance due to both specular and diffuse reflection and also spin conserving and spin-flip processes. It was shown that spin-flip scattering at interfaces substantially reduces the value of giant magnetoresistance (GMR). This can explain the experimental observations that the CPP GMR ratio for laminated structures only slightly increases as compared to non-laminated ones even though lamination induces a significant increase in CPP resistance

EFFECT OF LAMINATE INCLUSION AND THE TYPE OF ADHESIVE IN THE PROPERTIES OF OSB PANELS OF THE WOOD FROM Pinus oocarpa

Directory of Open Access Journals (Sweden)

José Benedito Guimarães júnior

2012-11-01

Full Text Available The aim of the study was to evaluate the influence of laminate inclusion and the type of adhesive on the physical and mechanical properties of OSB panels produced from the wood of Pinus oocarpa. The experimental design consisted of four treatments. Laminateinclusion and urea-formaldehyde and phenol-formaldehyde adhesives applied in combination with the panel layer were evaluated. For each treatment, three panels with nominal density of 0.65 g.cm-3, 1% to 6% paraffin and adhesive were produced. In the manufacturing of thepanels, temperature of 180 ° C, pressure of 3.95 MPa and pressing time of 8 minutes were adopted. From the results it is concluded that: the species Pinus oocarpa has great potential for use in the production of OSB panels; the laminate inclusion resulted in significant improvement in the properties of water absorption and thickness swelling after immersion for two hours, as well as in the modulus of elasticity and modulus of rupture in parallel direction; and the panels made with urea-formaldehyde adhesives in the core and phenol-formaldehydeon the faces statistically equal to panels only with the phenol-formaldehyde adhesive in statistically, except in the internal bonding property.

Autoclave processing for composite material fabrication. 1: An analysis of resin flows and fiber compactions for thin laminate

Science.gov (United States)

Hou, T. H.

1985-01-01

High quality long fiber reinforced composites, such as those used in aerospace and industrial applications, are commonly processed in autoclaves. An adequate resin flow model for the entire system (laminate/bleeder/breather), which provides a description of the time-dependent laminate consolidation process, is useful in predicting the loss of resin, heat transfer characteristics, fiber volume fraction and part dimension, etc., under a specified set of processing conditions. This could be accomplished by properly analyzing the flow patterns and pressure profiles inside the laminate during processing. A newly formulated resin flow model for composite prepreg lamination process is reported. This model considers viscous resin flows in both directions perpendicular and parallel to the composite plane. In the horizontal direction, a squeezing flow between two nonporous parallel plates is analyzed, while in the vertical direction, a poiseuille type pressure flow through porous media is assumed. Proper force and mass balances have been made and solved for the whole system. The effects of fiber-fiber interactions during lamination are included as well. The unique features of this analysis are: (1) the pressure gradient inside the laminate is assumed to be generated from squeezing action between two adjacent approaching fiber layers, and (2) the behavior of fiber bundles is simulated by a Finitely Extendable Nonlinear Elastic (FENE) spring.

Experimental Assessment of Tensile Failure Characteristic for Advanced Composite Laminates

Energy Technology Data Exchange (ETDEWEB)

Lee, Myoung Keon [Agency for Defense Development, Daejeon (Korea, Republic of); Lee, Jeong Won; Yoon, Dong Hyun; Kim, Jae Hoon [Chungnam Nat’l Univ., Daejeon (Korea, Republic of)

2017-10-15

In recent years, major airplane manufacturers have been using the laminate failure theory to estimate the strain of composite structures for airplanes. The laminate failure theory uses the failure strain of the laminate to analyze composite structures. This paper describes a procedure for the experimental assessment of laminate tensile failure characteristics. Regression analysis was used as the experimental assessment method. The regression analysis was performed with the response variable being the laminate failure strain and with the regressor variables being two-ply orientation (0° and ±45°) variables. The composite material in this study is a carbon/epoxy unidirectional (UD) tape that was cured as a pre-preg at 177°C(350°F). A total of 149 tension tests were conducted on specimens from 14 distinct laminates that were laid up at standard angle layers (0°, 45°, -45°, and 90°). The ASTM-D-3039 standard was used as the test method.

Experimental Assessment of Tensile Failure Characteristic for Advanced Composite Laminates

International Nuclear Information System (INIS)

Lee, Myoung Keon; Lee, Jeong Won; Yoon, Dong Hyun; Kim, Jae Hoon

2017-01-01

In recent years, major airplane manufacturers have been using the laminate failure theory to estimate the strain of composite structures for airplanes. The laminate failure theory uses the failure strain of the laminate to analyze composite structures. This paper describes a procedure for the experimental assessment of laminate tensile failure characteristics. Regression analysis was used as the experimental assessment method. The regression analysis was performed with the response variable being the laminate failure strain and with the regressor variables being two-ply orientation (0° and ±45°) variables. The composite material in this study is a carbon/epoxy unidirectional (UD) tape that was cured as a pre-preg at 177°C(350°F). A total of 149 tension tests were conducted on specimens from 14 distinct laminates that were laid up at standard angle layers (0°, 45°, -45°, and 90°). The ASTM-D-3039 standard was used as the test method.

Non-electric applications of pool-type nuclear reactors

International Nuclear Information System (INIS)

Adamov, E.O.; Cherkashov, Yu.M.; Romenkov, A.A.

1997-01-01

This paper recommends the use of pool-type light water reactors for thermal energy production. Safety and reliability of these reactors were already demonstrated to the public by the long-term operation of swimming pool research reactors. The paper presents the design experience of two projects: Apatity Underground Nuclear Heating Plant and Nuclear Sea-Water Desalination Plant. The simplicity of pool-type reactors, the ease of their manufacturing and maintenance make this type of a heat source attractive to the countries without a developed nuclear industry. (author). 6 figs, 1 tab

Numerical Investigation of Corrugated Wire Mesh Laminate

Directory of Open Access Journals (Sweden)

Jeongho Choi

2013-01-01

Full Text Available The aim of this work is to develop a numerical model of Corrugated Wire Mesh Laminate (CWML capturing all its complexities such as nonlinear material properties, nonlinear geometry and large deformation behaviour, and frictional behaviour. Development of such a model will facilitate numerical simulation of the mechanical behaviour of the wire mesh structure under various types of loading as well as the variation of the CWML configuration parameters to tailor its mechanical properties to suit the intended application. Starting with a single strand truss model consisting of four waves with a bilinear stress-strain model to represent the plastic behaviour of stainless steel, the finite element model is gradually built up to study single-layer structures with 18 strands of corrugated wire meshes consistency and double- and quadruple-layered laminates with alternating crossply orientations. The compressive behaviour of the CWML model is simulated using contact elements to model friction and is compared to the load-deflection behaviour determined experimentally in uniaxial compression tests. The numerical model of the CWML is then employed to conduct the aim of establishing the upper and lower bounds of stiffness and load capacity achievable by such structures.

Influence of bruxism on survival of porcelain laminate veneers.

Science.gov (United States)

Granell-Ruíz, Maria; Agustín-Panadero, Rubén; Fons-Font, Antonio; Román-Rodríguez, Juan-Luis; Solá-Ruíz, María-Fernanda

2014-09-01

This study aims to determine whether bruxism and the use of occlusal splints affect the survival of porcelain laminate veneers in patients treated with this technique. Restorations were made in 70 patients, including 30 patients with some type of parafunctional habit. A total of 323 veneers were placed, 170 in patients with bruxism activity, and the remaining 153 in patients without it. A clinical examination determined the presence or absence of ceramic failure (cracks, fractures and debonding) of the restorations; these incidents were analyzed for association with bruxism and the use of splints. Analysis of the ceramic failures showed that of the 13 fractures and 29 debonding that were present in our study, 8 fractures and 22 debonding were related to the presence of bruxism. Porcelain laminate veneers are a predictable treatment option that provides excellent results, recognizing a higher risk of failure in patients with bruxism activity. The use of occlusal splints reduces the risk of fractures.

The Effect of Thermal Lamination Processes on Colorimetric Change in Spot Colours

Directory of Open Access Journals (Sweden)

Eduard Galić

2015-03-01

Full Text Available Understanding the effect of laminating processes on spot colours is of great importance in the offset printing process, especially given the application versatility of spot colours. Laminating process, as a very common process and one of the first in a sequence of finishing processes in graphics production, can affect print’s visual impression to varying degrees. Spot colours, as mixtures of different ratios of inks, are subject to a change due to matt or gloss lamination process. The research examined the impact of thermal lamination processes on printed spot colours on different printing substrates. The degree of change on prints caused by laminating films in the thermal process was determined using spectrophotometric and densitometric methods. Particular emphasis is placed on the spot colour because of its specific characteristics. Research results are shown in charts and they are showing clearly the modality and the extent laminating processes effect the colorimetric difference in laminated and non-laminated prints. This scientific research provides objective conclusions that help in predicting the possible variations within the usage of laminating processes.

Modeling of Nonlinear Mechanical Response in CFRP Angle-Ply Laminates

Science.gov (United States)

Ogihara, Shinji

2014-03-01

It is known that the failure process in angle-ply laminate involves matrix cracking and delamination and that they exhibit nonlinear stress-strain relation. There may be a significant effect of the constituent blocked ply thickness on the mechanical behavior of angle-ply laminates. These days, thin prepregs whose thickness is, for example 50 micron, are developed and commercially available. Therefore, we can design wide variety of laminates with various constituent ply thicknesses. In this study, effects of constituent ply thickness on the nonlinear mechanical behavior and the damage behavior of CFRP angle-ply laminates are investigated experimentally. Based on the experimental results, the mechanical response in CFRP angle-ply laminates is modeled by using the finite strain viscoplasticity model. We evaluated the mechanical behavior and damage behavior in CFRP angle-ply laminates with different constituent ply thickness under tensile loading experimentally. It was found that as the constituent ply thickness decreases, the strength and failure strain increases. We also observed difference in damage behavior. The preliminary results of finite strain viscoplasticity model considering the damage effect for laminated composites are shown. A qualitative agreement is obtained.

Modeling transverse cracking in laminates with a single layer of elements per ply

NARCIS (Netherlands)

Van der Meer, F.P.; Davila, C.G.

2012-01-01

This study aims to bridge the gap between classical understanding of transverse cracking in [0/90]s laminates and recent computational methods for the modeling of progressive laminate failure. Specifically, the study investigates under which conditions a three-dimensional model with cohesive cracks

Effect of nanomodified polyester resin on hybrid sandwich laminates

International Nuclear Information System (INIS)

Anbusagar, NRR.; Giridharan, P.K.; Palanikumar, K.

2014-01-01

Highlights: • Effect of nanomodified polyester resin on hybrid sandwich laminates is evaluated. • The hybrid sandwich laminates are fabricated with varying wt% of nanoclay. • Flexural, impact and moisture absorbtion properties are evaluated for hybrid composites. • Scanning electron microscopy is utilized to analyze the dispersion of clay and fractured surfaces of the nanocomposites. - Abstract: Effect of nanoclay modified polyester resin on flexural, impact, hardness and water absorption properties of untreated woven jute and glass fabric hybrid sandwich laminates have been investigated experimentally. The hybrid sandwich laminates are prepared by hand lay-up manufacturing technique (HL) for investigation. All hybrid sandwich laminates are fabricated with a total of 10 layers, by varying the extreme layers and wt% of nanoclay in polyester resin so as to obtain four different combinations of hybrid sandwich laminates. For comparison of the composite with hybrid composite, jute fiber reinforced composite laminate also fabricated. X-ray diffraction (XRD) results obtained from samples with nanoclay indicated that intergallery spacing of the layered clay increases with matrix. Scanning electron microscopy (SEM) gave a morphological picture of the cross-sections and energy dispersive X-ray spectroscopy (EDS) allowed investigating the elemental composition of matrix in composites. The testing results indicated that the flexural properties are greatly increased at 4% of nanoclay loading while impact, hardness and water absorption properties are increased at 6% of nanoclay loading. A plausible explanation for high increase of properties has also been discussed

Analysis of a hybrid balanced laminate as a structural material for thick composite beams with axial stiffeners

Energy Technology Data Exchange (ETDEWEB)

Modak, Partha; Hossain, M. Jamil, E-mail: jamil917@gmail.com; Ahmed, S. Reaz [Department of Mechanical Engineering, Bangladesh University of Engineering and Technology, Dhaka 1000 (Bangladesh)

2016-07-12

An accurate stress analysis has been carried out to investigate the suitability of a hybrid balanced laminate as a structural material for thick composite beams with axial stiffeners. Three different balanced laminates composed of dissimilar ply material as well as fiber orientations are considered for a thick beam on simple supports with stiffened lateral ends. A displacement potential based elasticity approach is used to obtain the numerical solution of the corresponding elastic fields. The overall laminate stresses as well as individual ply stresses are analysed mainly in the perspective of laminate hybridization. Both the fiber material and ply angle of individual laminas are found to play dominant roles in defining the design stresses of the present composite beam.

Effects of differently hardened brass foil laminate on the electromechanical property of externally laminated CC tapes

Energy Technology Data Exchange (ETDEWEB)

Bautista, Zhierwinjay; Shin, Hyung Seop [Dept. of Mechanical Design Engineering, Andong National University, Andong (Korea, Republic of); Mean, Byoung Jean; Lee, Jae Hun [SuNAM Co Ltd., Anseong (Korea, Republic of)

2016-12-15

The mechanical properties of REBCO coated conductor (CC) wires under uniaxial tension are largely determined by the thick component layers in the architecture, namely, the substrate and the stabilizer or even the reinforcement layer. Depending on device applications of the CC tapes, it is necessary to reinforce thin metallic foils externally to one-side or both sides of the CC tapes. Due to the external reinforcement of brass foils, it was found that this could increase the reversible strain limit from the Cu-stabilized CC tapes. In this study, the effects of differently hardened brass foil laminate on the electromechanical property of CC tapes were investigated under uniaxial tension loading. The tensile strain dependence of the critical current (I{sub c}) was measured at 77 K and self-field. Depending on whether the I{sub c} of CC tapes were measured during loading or after unloading, a reversible strain (or stress) limit could be determined, respectively. The both-sides of the Cu-stabilized CC tapes were laminated with brass foils with different hardness, namely 1/4H, 1H and EH. From the obtained results, it showed that the yield strength of the brass laminated CC tapes with EH brass foil laminate was comparable to the one of the Cu-stabilized CC tape due to its large yield strength even though its large volume fraction. It was found that the brass foil with different hardness was mainly sensitive on the stress dependence of I{sub c}, but not on the strain sensitivity due to the residual strain induced in the laminated CC tapes during unloading.

Laminated Ti-Al composites: Processing, structure and strength

DEFF Research Database (Denmark)

Du, Yan; Fan, Guohua; Yu, Tianbo

2016-01-01

Laminated Ti-Al composite sheets with different layer thickness ratios have been fabricated through hot pressing followed by multi-pass hot rolling at 500 °C.The laminated sheets show strong bonding with intermetallic interface layers of nanoscale thickness between the layers of Ti and Al....... The mechanical properties of the composites with different volume fractions of Al from 10% to 67% show a good combination of strength and ductility. A constraint strain in the hot-rolled laminated structure between the hard and soft phases introduces an elastic-plastic deformation stage, which becomes more...

Comparative study T-type and I-type layout of PWR nuclear power plants

International Nuclear Information System (INIS)

Eko Rudi Iswanto and Siti Alimah

2010-01-01

Determining plant layout is one of the five major stages during the life time of a nuclear power plant. Some important factors that affect in the selecting of plant layout are availability of infrastructure, economic aspects, social aspects, public and environment safety, and also easy to do. Another factor to be considered is requirements as seismic design, which refers to the principles of good security workers, communities and the environment of radiological risks. There are many layout types of nuclear power plant, two of them are T-type layout and I-type layout. Each type of the plant layout has advantage and disadvantage, therefore this study is to understand them. Good layout is able to provide a high level of security against earthquakes. In term of earthquake design, I-type layout has a higher security level than T-type layout. Therefore, I-type layout can be a good choice for PWR nuclear power plants 1000 MWe that will be built in Indonesia. (author)

Water cooled type nuclear power plant

International Nuclear Information System (INIS)

Arai, Shigeki.

1981-01-01

Purpose: To construct high efficiency a PWR type nuclear power plant with a simple structure by preparing high temperature and pressure water by a PWR type nuclear reactor and a pressurizer, converting the high temperature and high pressure water into steam with a pressure reducing valve and introducing the steam into a turbine, thereby generating electricity. Constitution: A pressurizer is connected downstream of a PWR type nuclear reactor, thereby maintaining the reactor at high pressure. A pressure-reducing valve is provided downstream of the pressurizer, the high temperature and pressure water is reduced in pressure, thereby producing steam. The steam is fed to a turbine, and electric power is generated by a generator connected to the turbine. The steam exhausted from the turbine is condensed by a condenser into water, and the water is returned through a feedwater heater to the reactor. Since the high temperature and pressure water in thus reduced in pressure thereby evaporating it, the steam can be more efficiently produced than by a steam generator. (Sekiya, K.)

Tensile strength of glulam laminations of Nordic spruce

DEFF Research Database (Denmark)

Hoffmeyer, Preben; Bräuner, Lise; Boström, Lars

1999-01-01

Design of glulam according to the European timber code Eurocode 5 is based on the standard document prEN1194 , according to which glulam beam strength is to be established either by full scale testing or by calculation. The calculation must be based on a knowledge of lamination tensile strength....... This knowledge may be obtained either by adopting a general rule that the characteristic tensile strength is sixty percent of the characteristic bending strength, or by performing tensile tests on an adequate number of laminations representative of the whole population. The present paper presents...... an investigation aimed at establishing such an adequate experimental background for the assignment of strength classes for glulam made of visually strength graded laminations from Nordic sawmills. The investigation includes more than 1800 boards (laminations) of Norway spruce (Picea abies) sampled from eight...

A Step-by-Step Conservative Approach for CAD-CAM Laminate Veneers

Directory of Open Access Journals (Sweden)

Gerardo Durán Ojeda

2017-01-01

Full Text Available The use of CAD/CAM technology has allowed the fabrication of ceramic restorations efficiently and with predictable results. Lithium disilicate is a type of glass ceramic material that can be used for the elaboration of laminate veneers, being monolithic restorations which require characterization through a covering ceramic in order to achieve acceptable esthetic results. The next case report shows a predictable clinical protocol for the rehabilitation of the anterior teeth through the preparation of CAD/CAM veneers (e.max CAD, Ivoclar Vivadent, Liechtenstein which have been characterized by a nanofluorapatite ceramic (e.max Ceram, Ivoclar Vivadent, Liechtenstein through the layering technique.

Generation of micronuclei during interphase by coupling between cytoplasmic membrane blebbing and nuclear budding.

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Koh-ichi Utani

Full Text Available Micronucleation, mediated by interphase nuclear budding, has been repeatedly suggested, but the process is still enigmatic. In the present study, we confirmed the previous observation that there are lamin B1-negative micronuclei in addition to the positive ones. A large cytoplasmic bleb was found to frequently entrap lamin B1-negative micronuclei, which were connected to the nucleus by a thin chromatin stalk. At the bottom of the stalk, the nuclear lamin B1 structure appeared broken. Chromatin extrusion through lamina breaks has been referred to as herniation or a blister of the nucleus, and has been observed after the expression of viral proteins. A cell line in which extrachromosomal double minutes and lamin B1 protein were simultaneously visualized in different colors in live cells was established. By using these cells, time-lapse microscopy revealed that cytoplasmic membrane blebbing occurred simultaneously with the extrusion of nuclear content, which generated lamin B1-negative micronuclei during interphase. Furthermore, activation of cytoplasmic membrane blebbing by the addition of fresh serum or camptothecin induced nuclear budding within 1 to 10 minutes, which suggested that blebbing might be the cause of the budding. After the induction of blebbing, the frequency of lamin-negative micronuclei increased. The budding was most frequent during S phase and more efficiently entrapped small extrachromosomal chromatin than the large chromosome arm. Based on these results, we suggest a novel mechanism in which cytoplasmic membrane dynamics pulls the chromatin out of the nucleus through the lamina break. Evidence for such a mechanism was obtained in certain cancer cell lines including human COLO 320 and HeLa. The mechanism could significantly perturb the genome and influence cancer cell phenotypes.

Thermally induced self-healing epoxy/glass laminates with porous layers containing crystallized healing agent

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T. Szmechtyk

2018-07-01

Full Text Available Porous glass fiber and paper layers were tested for application in thermally induced self healing epoxy laminates as healing porous layers. Both types of layers were impregnated using high purity bisphenol A diglycidyl ether (BADGE epoxy with ability to crystallize during storage under 25 °C. Absorption capacity of porous layers was evaluated. Differential scanning calorimetry was used to investigate BADGE healing agent recrystallization process. Healing porous glass layers (HPGL were selected for further tests. Liquid chromatography and Fourier transform infrared (FT IR spectroscopy provided information about average molecular mass of embedded healing agent and functional groups in HPGL layers. Self-healing efficiency of three different laminates with HPGL layers was calculated based on the results of three-point bending test and Charpy impact test. Also, flexural properties and impact strength of laminates were evaluated. The obtained results confirm competitive self healing ability of composites with HPGL.

Electrical behavior of laminated composites with intralaminar degradation: A comprehensive micro-meso homogenization procedure

KAUST Repository

Selvakumaran, Lakshmi; Lubineau, Gilles

2014-01-01

Electrical Resistance Tomography (ERT) is a promising health monitoring technique to assess damage in laminated composites. Yet, the missing link between the various complex degradation mechanisms within the laminate and its global change

Analytical and Experimental Characterization of Thick-Section Fiber-Metal Laminates

Science.gov (United States)

2013-06-01

laminate . The model individually models each layer of the laminate and predicts stiffness degradation as metal layers plastically deform and as prepreg ...eliminating four of the possible ECM laminates . Additionally, since at least four individual layers (two aluminum and two prepreg ) are used in FML an...AFRL-AFOSR-UK-TR-2013-0023 Analytical and Experimental Characterization of Thick- Section Fiber-Metal Laminates Dr. Rene

Convolute laminations — a theoretical analysis: example of a Pennsylvanian sandstone

Science.gov (United States)

Visher, Glenn S.; Cunningham, Russ D.

1981-03-01

Data from an outcropping laminated interval were collected and analyzed to test the applicability of a theoretical model describing instability of layered systems. Rayleigh—Taylor wave perturbations result at the interface between fluids of contrasting density, viscosity, and thickness. In the special case where reverse density and viscosity interlaminations are developed, the deformation response produces a single wave with predictable amplitudes, wavelengths, and amplification rates. Physical measurements from both the outcropping section and modern sediments suggest the usefulness of the model for the interpretation of convolute laminations. Internal characteristics of the stratigraphic interval, and the developmental sequence of convoluted beds, are used to document the developmental history of these structures.

Lamin B Receptor: Interplay between Structure, Function and Localization

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Eleni Nikolakaki

2017-08-01

Full Text Available Lamin B receptor (LBR is an integral protein of the inner nuclear membrane, containing a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail. Two seemingly unrelated functions have been attributed to LBR. Its N-terminal domain tethers heterochromatin to the nuclear periphery, thus contributing to the shape of interphase nuclear architecture, while its transmembrane domains exhibit sterol reductase activity. Mutations within the transmembrane segments result in defects in cholesterol synthesis and are associated with diseases such as the Pelger–Huët anomaly and Greenberg skeletal dysplasia, whereas no such harmful mutations related to the anchoring properties of LBR have been reported so far. Recent evidence suggests a dynamic regulation of LBR expression levels, structural organization, localization and function, in response to various signals. The molecular mechanisms underlying this dynamic behavior have not yet been fully unraveled. Here, we provide an overview of the current knowledge of the interplay between the structure, function and localization of LBR, and hint at the interconnection of the two distinct functions of LBR.

Deflection of Cross-Ply Composite Laminates Induced by Piezoelectric Actuators

Directory of Open Access Journals (Sweden)

Chi-Sheng Lin

2010-01-01

Full Text Available The coupling effects between the mechanical and electric properties of piezoelectric materials have drawn significant attention for their potential applications as sensors and actuators. In this investigation, two piezoelectric actuators are symmetrically surface bonded on a cross-ply composite laminate. Electric voltages with the same amplitude and opposite sign are applied to the two symmetric piezoelectric actuators, resulting in the bending effect on the laminated plate. The bending moment is derived by using the classical laminate theory and piezoelectricity. The analytical solution of the flexural displacement of the simply supported composite plate subjected to the bending moment is solved by using the plate theory. The analytical solution is compared with the finite element solution to show the validation of present approach. The effects of the size and location of the piezoelectric actuators on the response of the composite laminate are presented through a parametric study. A simple model incorporating the classical laminate theory and plate theory is presented to predict the deformed shape of the simply supported laminate plate.

Effect of shallow angles on compressive strength of biaxial and triaxial laminates.

Science.gov (United States)

Jia, Hongli; Yang, Hyun-Ik

2016-01-01

Biaxial (BX) and triaxial (TX) composite laminates with ±45° angled plies have been widely used in wind turbine blades. As the scale of blades increases, BX and TX laminates with shallow-angled plies (i.e. off-axis ply angle shallow-angled BX and TX laminates are critical considering their locations in a wind turbine blade, and therefore in this study, the uniaxial static compression tests were conducted using BX and TX laminates with angled-plies of ±45°, ±35°, and ±25°, for the purpose of evaluation. On the other hand, Mori-Tanaka mean field homogenization method was employed to predict elastic constants of plies in BX and TX laminates involved in tests; linear regression analyses of experimentally measured ply strengths collected from various sources were then performed to estimate strengths of plies in BX and TX laminates; finally, Tsai-Wu, Hashin, and Puck failure criteria were chosen to predict compressive strengths of BX and TX laminates. Comparison between theoretical predictions and test results were carried out to illustrate the effectiveness of each criterion. The compressive strength of BX laminate decreases as ply angle increases, and the trend was successfully predicted by all three failure criteria. For TX laminates, ±35° angled plies rather than ±45° angled plies led to the lowest laminate compressive strength. Hashin and Puck criteria gave good predictions at certain ply angles for TX laminates, but Tsai-Wu criterion was able to capture the unexpected strength variation of TX laminates with ply angle. It was concluded that the transverse tensile stress in 0° plies of TX laminates, which attains its maximum when the off-axis ply angle is 35°, is the dominant factor in failure determination if using Tsai-Wu criterion. This explains the unexpected strength variation of TX laminates with ply angle, and also indicates that proper selection of ply angle is the key to fully utilizing the advantages of shallow-angled laminates.

Lamination effects on a 3D model of the magnetic core of power transformers

Directory of Open Access Journals (Sweden)

Poveda-Lerma Antonio

2017-12-01

Full Text Available In this paper the lamination effect on the model of a power transformer’s core with stacked E-I structure is analyzed. The distribution of the magnetic flux in the laminations depends on the stacking method. In this work it is shown, using a 3D FEM model and an experimental prototype, that the non-uniform distribution of the flux in a laminated E-I core with alternate-lap joint stack increases substantially the average value of the magnetic flux density in the core, compared with a butt joint stack. Both the simulated model and the experimental tests show that the presence of constructive air-gaps in the E-I junctions gives rise to a zig-zag flux in the depth direction. This inter-lamination flux reduces the magnetic flux density in the I-pieces and increases substantially the magnetic flux density in the E-pieces, with highly saturated points that traditional 2D analysis cannot reproduce. The relation between the number of laminations included in the model, and the computational resourses needed to build it, is also evaluated in this work.

Postmortal radiographic diagnosis of laminitis in a captive European moose (Alces alces).

Science.gov (United States)

Clauss, M; Keller, A; Peemöller, A; Nygrén, K; Hatt, J-M; Nuss, K

2009-11-01

A five year-old bull moose (Alces alces) was culled due to chronic hoof overgrowth that required frequent intervention. Radiographic examination revealed changes in phalangeal bone structure usually considered indicative for laminitis in domestic cattle; similar changes were absent in the hooves of a free-ranging moose of similar age. The captive animal had been maintained in exhibits whose flooring were much harder than the soil in natural moose habitat, and on a diet with a high proportion of easily fermentable carbohydrates. These findings indicate that chronic laminitis should be considered as a potential underlying factor for hoof overgrowth, and that measures aimed at reducing the incidence of laminitis in domestic cattle, such as the use of softer flooring and diets with a higher proportion of fibre, might have prophylactic potential in captive wild ruminants.

Fatigue Performance of Composite Laminates After Low-velocity Impact

Directory of Open Access Journals (Sweden)

LIANG Xiao-lin

2016-12-01

Full Text Available Compression-compression fatigue tests were carried out on T300/5405 composite laminates after low-velocity impact, compression performance of the laminates with different impact damages was studied together with its fatigue life and damage propagation under different stress levels, then the effects of impact energy, stress level and damage propagation on fatigue life of laminates were discussed. The results indicate that impact damage can greatly reduce the residual strength of laminates; under low fatigue load levels, the higher impact energy is, the shorter the fatigue life of laminates with impact damage will be; damage propagation undergoes two stages during the fatigue test, namely the steady propagation and the rapid propagation, accounting for 80% and 20% of the overall fatigue life, respectively; damage propagation rate decreases with the reduction of stress level.

Low Drift Type N Thermocouples for Nuclear Applications

International Nuclear Information System (INIS)

Scervini, M.; Rae, C.

2013-06-01

Thermocouples are the most commonly used sensors for temperature measurement in nuclear reactors. They are crucial for the control of current nuclear reactors and for the development of GEN IV reactors. In nuclear applications thermocouples are strongly affected by intense neutron fluxes. As a result of the interaction with neutrons, the thermoelements of the thermocouples undergo transmutation, which produces a time dependent change in composition and, as a consequence, a time dependent drift of the thermocouple signal. Thermocouple drift can be very significant for in-pile temperature measurements and may render the temperature sensors unreliable after exposure to nuclear radiation for relatively short times compared to the life required for temperature sensors in nuclear applications. Previous experiences with type K thermocouples in nuclear reactors have shown that they are affected by neutron irradiation only to a limited extent. Similarly type N thermocouples are expected to be only slightly affected by neutron fluxes. Currently the use of Nickel based thermocouples is limited to temperatures lower than 1000 deg. C due to drift related to phenomena other than nuclear irradiation. In this work, undertaken as part of the European project METROFISSION, the drift of type N thermocouples has been investigated in the temperature range 600-1300 deg. C. The approach of this study is based on the attempt to separate the contributions of each thermo-element to drift. In order to identify the dominant thermo-element for drift, the contributions of both positive (NP) and negative (NN) thermo-elements to the total drift of 3.2 mm diameter MIMS thermocouples have been measured in each drift test using a pure Pt thermo-element as a reference. Conventional Inconel-600 sheathed type N thermocouples have been compared with type N thermocouples sheathed in a new alloy. At temperatures higher than 1000 deg. C conventional Inconel600 sheathed type N thermocouples can experience a

Electromagnetic Acoustic Test of the Artificial Defects for a Plate-type Nuclear Fuel

International Nuclear Information System (INIS)

Jung, Hyun Kyu; Kim, Dong Min; Lee, Yoon Sang; Cheong, Yong Moo

2011-01-01

Most research and test reactors use the nuclear fuel plates which are consisted of a fuel meat in aluminum alloy. Last year, KAERI signed a deal with the Jordan Atomic Energy Commission to build the research reactor and have to supply the plate-type nuclear fuels. For the demands of world market, KAERI started the research and development of the plate-type fuel elements and endeavored to achieve a localization of the plate-type fuel fabrication. For the inspection of plate-type fuel elements to be used in Research Reactors, an immersion pulse-echo ultrasonic technique was applied. This inspection was done under immersion condition, so a nuclear fuel was immersed to be prone to corrosion and needed to have time and cost due to an additional process. The sample that will be examined is a non-ferromagnetic material such as aluminum with a good acousto-elastic property, which requires an effective inspection of a bond quality for a nuclear fuel under a manufacturing environment. The purpose of this study is to investigate the feasibility of an Electromagnetic Acoustic Transducer (EMAT) technology for an automated inspection of a nuclear fuel without water

Analysis of 3D Planar Laminations in a Welded Section of API 5L X52 Applying the Finite Element Method

Directory of Open Access Journals (Sweden)

Martin. J. Fernández-Cueto

Full Text Available Abstract The mechanical behavior of API 5L X52 steel with planar type laminations was studied in the present work. Planar laminations were proposed in the base metal (BM, heat affected zone (HAZ and welding bead (WB. Three-dimensional finite element (FE models, kinematic hardening and mechanical properties for BM, HAZ, and WB were activated in the finite element program. The results showed that crack propagation corresponds to the direction of the main stress. For a crack length (2a of 10.01 mm, crack propagation may occur at the right crack tip towards the outer wall of the BM. For 2a of 15.12 mm, crack propagation was located on the right crack tip and propagates to the inner wall. For 2a of 17.12 mm, crack propagation was observed at the left crack tip and propagates to the outer wall in the BM. The results achieved by FEM agree when compared with real laminations in API 5L pipelines.

Concurrent material-fabrication optimization of metal-matrix laminates under thermo-mechanical loading

Science.gov (United States)

Saravanos, D. A.; Morel, M. R.; Chamis, C. C.

1991-01-01

A methodology is developed to tailor fabrication and material parameters of metal-matrix laminates for maximum loading capacity under thermomechanical loads. The stresses during the thermomechanical response are minimized subject to failure constrains and bounds on the laminate properties. The thermomechanical response of the laminate is simulated using nonlinear composite mechanics. Evaluations of the method on a graphite/copper symmetric cross-ply laminate were performed. The cross-ply laminate required different optimum fabrication procedures than a unidirectional composite. Also, the consideration of the thermomechanical cycle had a significant effect on the predicted optimal process.

Accumulation of distinct prelamin A variants in human diploid fibroblasts differentially affects cell homeostasis

International Nuclear Information System (INIS)

Candelario, Jose; Borrego, Stacey; Reddy, Sita; Comai, Lucio

2011-01-01

Lamin A is a component of the nuclear lamina that plays a major role in the structural organization and function of the nucleus. Lamin A is synthesized as a prelamin A precursor which undergoes four sequential post-translational modifications to generate mature lamin A. Significantly, a large number of point mutations in the LMNA gene cause a range of distinct human disorders collectively known as laminopathies. The mechanisms by which mutations in lamin A affect cell function and cause disease are unclear. Interestingly, recent studies have suggested that alterations in the normal lamin A pathway can contribute to cellular dysfunction. Specifically, we and others have shown, at the cellular level, that in the absence of mutations or altered splicing events, increased expression of wild-type prelamin A results in a growth defective phenotype that resembles that of cells expressing the mutant form of lamin A, termed progerin, associated with Hutchinson-Gilford Progeria syndrome (HGPS). Remarkably, the phenotypes of cells expressing elevated levels of wild-type prelamin A can be reversed by either treatment with farnesyltransferase inhibitors or overexpression of ZMPSTE24, a critical prelamin A processing enzyme, suggesting that minor increases in the steady-state levels of one or more prelamin A intermediates is sufficient to induce cellular toxicity. Here, to investigate the molecular basis of the lamin A pathway toxicity, we characterized the phenotypic changes occurring in cells expressing distinct prelamin A variants mimicking specific prelamin A processing intermediates. This analysis demonstrates that distinct prelamin A variants differentially affect cell growth, nuclear membrane morphology, nuclear distribution of lamin A and the fundamental process of transcription. Expression of prelamin A variants that are constitutively farnesylated induced the formation of lamin A aggregates and dramatic changes in nuclear membrane morphology, which led to reduced

Fatigue damage mechanics of notched graphite-epoxy laminates

Science.gov (United States)

Spearing, Mark; Beaumont, Peter W. R.; Ashby, Michael F.

A modeling approach is presented that recognizes that the residual properties of composite laminates after any form of loading depend on the damage state. Therefore, in the case of cyclic loading, it is necessary to first derive a damage growth law and then relate the residual properties to the accumulated damage. The propagation of fatigue damage in notched laminates is investigated. A power law relationship between damage growth and the strain energy release rate is developed. The material constants used in the model have been determined in independent experiments and are invariant for all the layups investigated. The strain energy release rates are calculated using a simple finite element representation of the damaged specimen. The model is used to predict the effect of tension-tension cyclic loading on laminates of the T300/914C carbon-fiber epoxy system. The extent of damage propagation is successfully predicted in a number of cross-ply laminates.

Hygrothermal effects on the tensile strength of carbon/epoxy laminates with molded edges

Directory of Open Access Journals (Sweden)

Cândido Geraldo Maurício

2000-01-01

Full Text Available The interlaminar stresses are confined to a region near the free edge. Therefore, the laminate stacking sequence and the free edge finishing are some of the factors that affect the strength of the laminate and limit its life. The use of molded edges eliminates the need for trimming and machining the laminates edges thus improving productivity. However, this fabrication technique may have a detrimental effect on the laminate strength for certain stacking sequences. This effect in the presence of moisture has not been characterized. This work presents the results of a comparative study of the resistance to delamination of laminates with machined edges and molded edges. Additionally, two environmental conditions were considered: dry laminates and laminates saturated with moisture. The tensile strength of the laminates were measured and micrographs were used to analyze the microstructure of the laminates near the free edges. It is concluded that the mechanical properties of advanced composites depend on the environmental conditions and the fabrication techniques used to produce the laminates. Therefore, it is necessary to account for these factors when experimentally determining the design allowables.

Finite elements modeling of delaminations in composite laminates

DEFF Research Database (Denmark)

Gaiotti, m.; Rizzo, C.M.; Branner, Kim

2011-01-01

of the buckling strength of composite laminates containing delaminations. Namely, non-linear buckling and post-buckling analyses are carried out to predict the critical buckling load of elementary composite laminates affected by rectangular delaminations of different sizes and locations, which are modelled......The application of composite materials in many structures poses to engineers the problem to create reliable and relatively simple methods, able to estimate the strength of multilayer composite structures. Multilayer composites, like other laminated materials, suffer from layer separation, i.......e., delaminations, which may affect the stiffness and stability of structural components. Especially deep delaminations in the mid surface of laminates are expected to reduce the effective flexural stiffness and lead to collapse, often due to buckling behaviour. This paper deals with the numerical modelling...

Laser cutting of Kevlar laminates

Energy Technology Data Exchange (ETDEWEB)

VanCleave, R.A.

1977-09-01

An investigation has been conducted of the use of laser energy for cutting contours, diameters, and holes in flat and shaped Kevlar 49 fiber-reinforced epoxy laminates as an alternate to conventional machining. The investigation has shown that flat laminates 6.35 mm thick may be cut without backup by using a high-powered (1000-watt) continuous wave CO/sub 2/ laser at high feedrates (33.87 mm per second). The cut produced was free of the burrs and delaminations resulting from conventional machining methods without intimate contact backup. In addition, the process cycle time was greatly reduced.

Investigation of Forming Performance of Laminated Steel Sheets Using Finite Element Analyses

International Nuclear Information System (INIS)

Liu Wenning; Sun Xin; Ruokolainen, Robert; Gayden Xiaohong

2007-01-01

Laminated steel sheets have been used in automotive structures for reducing in-cabin noise. However, due to the marked difference in material properties of the different laminated layers, integrating laminated steel parts into the manufacturing processes can be challenging. Especially, the behavior of laminated sheets during forming processes is very different from that of monolithic steel sheets. During the deep-draw forming process, large shear deformation and corresponding high interfacial stress may initiate and propagate interfacial cracks between the core polymer and the metal skin, hence degrading the performance of the laminated sheets. In this paper, the formability of the laminated steel sheets is investigated by means of numerical analysis. The goal of this work is to gain insight into the relationship between the individual properties of the laminated sheet layers and the corresponding formability of the laminated sheet as a whole, eventually leading to reliable design and successful forming process development of such materials. Finite element analyses of laminate sheet forming are presented. Effects of polymer core thickness and viscoelastic properties of the polymer core, as well as punching velocity, are also investigated

Recycling of waste automotive laminated glass and valorization of polyvinyl butyral through mechanochemical separation

International Nuclear Information System (INIS)

Swain, Basudev; Ryang Park, Jae; Yoon Shin, Dong; Park, Kyung-Soo; Hwan Hong, Myung; Gi Lee, Chan

2015-01-01

Due to strong binding, optical clarity, adhesion to many surfaces, toughness and flexibility polyvinyl butyral (PVB) resin films are commonly used in the automotive and architectural application as a protective interlayer in the laminated glass. Worldwide million tons of PVB waste generated from end-of-life automotive associated with various environmental issues. Stringent environmental directive, higher land cost eliminates land filling option, needs a study, we have developed a mechanochemical separation process to separate PVB resins from glass and characterized the separated PVB through various techniques, i.e., scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), infrared spectroscopy (IR) and nuclear magnetic resonance spectroscopy (NMR). Commercial nonionic surfactants D201 used for the mechanochemical separation purpose. Through parameter optimization following conditions are considered to be the optimum condition; 30 vol% D201, stirring speed of 400 rpm, 35 °C temperature, operation time 1 h, and dilute D201 volume to waste automotive laminated glass weight ratio of ≈25. The technology developed in our laboratory is sustainable, environmentally friendly, techno-economical feasible process, capable of mass production (recycling). - Highlights: • Waste automotive laminated glass and polyvinyl butyral mechanochemically separated. • An economical total recovery and environment-friendly process has been developed. • It is a global problem rather than regional environmental issue has been addressed. • Without using hazardous chemical wastes are being converted to a wealth.

On the plastic behaviour of multi directional epoxy-bolted CFRP laminates

DEFF Research Database (Denmark)

Jensen, Aage; Poulsen, Ervin

2004-01-01

The second generation of CFRP laminate has recently been developed. It is a multi directional CFRP laminate, i.e. a laminate with carbon fibres having several directions other than the first generation. The paper describes the laboratory tests carried out in order to develop anchorage devices for...

Laminitis with severe rarefactive osteitis of the distal phalanx in a young Simmental bull

International Nuclear Information System (INIS)

Kofler, J.; Stanek, C.

1990-01-01

A case report of a 9-month-old Simmental bull with chronic laminitis is presented including clinical examination, radiology and pathomorphology. The condition is similar to several cases of laminitis described earlier in Jersey cattle, which have been attributed to hereditary factors

Suspension state increases reattachment of breast cancer cells by up-regulating lamin A/C.

Science.gov (United States)

Zhang, Xiaomei; Lv, Yonggang

2017-12-01

Extravasation is a rate-limiting step of tumor metastasis, for which adhesion to endothelium of circulating tumor cells (CTCs) is the prerequisite. The suspension state of CTCs undergoing detachment from primary tumor is a persistent biomechanical cue, which potentially regulates the biophysical characteristics and cellular behaviors of tumor cells. In this study, breast tumor cells MDA-MB-231 in suspension culture condition were used to investigate the effect of suspension state on reattachment of CTCs. Our study demonstrated that suspension state significantly increased the adhesion ability of breast tumor cells. In addition, suspension state markedly promoted the formation of stress fibers and focal adhesions and reduced the motility in reattached breast cancer cells. Moreover, lamin A/C was reversibly accumulated at posttranscriptional level under suspension state, improving the cell stiffness of reattached breast cancer cells. Disruption of actin cytoskeleton by cytochalasin D caused lamin A/C accumulation. Conversely, decreasing actomyosin contraction by ROCK inhibitor Y27632 reduced lamin A/C level. Knocking down lamin A/C weakened the suspension-induced increase of adhesion, and also abolished the suspension-induced decrease of motility and increase of stress fibers and focal adhesion in reattaching tumor cells, suggesting a crucial role of lamin A/C. In conclusion, it was demonstrated that suspension state promoted the reattachment of breast tumor cells by up-regulating lamin A/C via cytoskeleton disruption. These findings highlight the important role of suspension state for tumor cells in tumor metastasis. Copyright © 2017 Elsevier B.V. All rights reserved.

Normalization of Impact Energy by Laminate Thickness for Compression After Impact Testing

Science.gov (United States)

Nettles, A. T.; Hromisin, S. M.

2013-01-01

The amount of impact energy used to damage a composite laminate is a critical parameter when assessing residual strength properties. The compression after impact (CAI) strength of impacted laminates is dependent upon how thick the laminate is and this has traditionally been accounted for by normalizing (dividing) the impact energy by the laminate's thickness. However, when comparing CAI strength values for a given lay-up sequence and fiber/resin system, dividing the impact energy by the specimen thickness has been noted by the author to give higher CAI strength values for thicker laminates. A study was thus undertaken to assess the comparability of CAI strength data by normalizing the impact energy by the specimen thickness raised to a power to account for the higher strength of thicker laminates. One set of data from the literature and two generated in this study were analyzed by dividing the impact energy by the specimen thickness to the 1, 1.5, 2, and 2.5 powers. Results show that as laminate thickness and damage severity decreased, the value which the laminate thickness needs to be raised to in order to yield more comparable CAI data increases.

Plated lamination structures for integrated magnetic devices

Science.gov (United States)

Webb, Bucknell C.

2014-06-17

Semiconductor integrated magnetic devices such as inductors, transformers, etc., having laminated magnetic-insulator stack structures are provided, wherein the laminated magnetic-insulator stack structures are formed using electroplating techniques. For example, an integrated laminated magnetic device includes a multilayer stack structure having alternating magnetic and insulating layers formed on a substrate, wherein each magnetic layer in the multilayer stack structure is separated from another magnetic layer in the multilayer stack structure by an insulating layer, and a local shorting structure to electrically connect each magnetic layer in the multilayer stack structure to an underlying magnetic layer in the multilayer stack structure to facilitate electroplating of the magnetic layers using an underlying conductive layer (magnetic or seed layer) in the stack as an electrical cathode/anode for each electroplated magnetic layer in the stack structure.

Influence of Different Types of Resin Luting Agents on Color Stability of Ceramic Laminate Veneers Subjected to Accelerated Artificial Aging.

Science.gov (United States)

Silami, Francisca Daniele Jardilino; Tonani, Rafaella; Alandia-Román, Carla Cecilia; Pires-de-Souza, Fernanda de Carvalho Panzeri

2016-01-01

The aim of this study was to evaluate the influence of accelerated aging (AAA) on the color stability of resin cements for bonding ceramic laminate veneers of different thicknesses. The occlusal surfaces of 80 healthy human molars were flattened. Ceramic laminate veneers (IPS e-max Ceram) of two thicknesses (0.5 and 1.0 mm) were bonded with three types of luting agents: light-cured, conventional dual and self-adhesive dual cement. Teeth without restorations and cement samples (0.5 mm) were used as control. After initial color evaluations, the samples were subjected to AAA for 580 h. After this, new color readouts were made, and the color stability (ΔE) and luminosity (ΔL) data were analyzed. The greatest color changes (p<0.05) occurred when 0.5 mm veneers were fixed with light-cured cement and the lowest when 1.0 mm veneers were fixed with conventional dual cement. There was no influence of the restoration thickness when the self-adhesive dual cement was used. When veneers were compared with the control groups, it was verified that the cement samples presented the greatest alterations (p<0.05) in comparison with both substrates and restored teeth. Therefore, it was concluded that the thickness of the restoration influences color and luminosity changes for conventional dual and light-cured cements. The changes in self-adhesive cement do not depend on restoration thickness.

Study of the Formability of Laminated Lightweight Metallic Materials

Directory of Open Access Journals (Sweden)

Girjob Claudia

2017-01-01

Full Text Available The main objective of this work was to test the formability of laminated materials. Laminated materials are considered a good choice when parts with reduced weight are considered. Thus, a laminated material, aluminum - polypropylene - aluminum (Al-PP-Al, as sheet 1.2 mm and 1.4 mm thickness was used. Before processing the material by means of unconventional plastic deformation, its formability was determined by running the Nakajima test. After obtaining the forming limit curves, the material was machined by means of incremental forming.

Case-control study of risk factors for the development of laminitis in the contralateral limb in Equidae with unilateral lameness

International Nuclear Information System (INIS)

Peloso, J.G.; Cohen, N.D.; Walker, M.A.; Watkins, J.P.; Gayle, J.M.; Moyer, W.

1996-01-01

To identify risk factors associated with development of laminitis of the supporting limb in Equidae with unilateral laminitis and to determine the radiographic appearance of this type of laminitis. Retrospective analysis of medical records. 20 Equidae with unilateral lameness that developed laminitis of the contralateral limb. Case animals were compared with matched and unmatched populations of control animals that did not develop contralateral limb laminitis. Lateromedial radiographic projections of affected feet were evaluated for evidence of laminitis. Body weight of case animals was not significantly different from that of control animals, but number of days that control animals were lame prior to recovery was significantly less than number of days that case animals were lame prior to the onset of laminitis. Lateromedial radiographic projections of the foot of the support limb were available for 16 of the 20 case animals. For all 16, thickness of the soft tissue dorsal to the distal phalanx was > 29% of the palmar cortical length of the distal phalanx, but only 1 had evidence of rotation of the distal phalanx. The proportion of case animals that were euthanatized was significantly greater than the proportion of control animals that were euthanatized. Duration of lameness, but not body weight, was a risk factor for development of laminitis in the contralateral limb in Equidae with unilateral lameness, and animals that developed this complication were more likely to be euthanatized than were animals that did not

Sound insulation of composite cylindrical shells: a comparison between a laminated and a sandwich cylinder

OpenAIRE

Yuan, Chongxin; Roozen, Bert; Bergsma, Otto; Beukers, Adriaan

2012-01-01

The fuselages of aircraft are modeled as a cylinder in this paper, and the sound insulations of a sandwich cylinder and a laminated cylinder are studied both experimentally and numerically. The cylinders are excited by an acoustic pressure and a mechanical force respectively. Results show that under acoustic excitation, the sandwich cylinder and the laminated one have a similar sound insulation below 3000 Hz, but the sandwich cylinder has a much larger sound insulation at higher frequencies. ...

Effect of matrix cracking on the time delayed buckling of viscoelastic laminated circular cylindrical shells

Institute of Scientific and Technical Information of China (English)

PENG Fan; FU YiMing; CHEN YaoJun

2008-01-01

The effect of matrix cracking on the bifurcation creep buckling of viscoelastic laminated circular cylindrical shells is investigated. The viscoelastic behavior of laminas is modeled by Schapery's integral constitutive equation with growing ma-trix cracks. The values of damage variables are correlated to non-dimensional density of matrix cracks relying on the formulas from meso-mechanics approach, and the evolution equation predicting the growth rate of density of matrix cracks is assumed to follow a power type relation with transverse tensile stress. The gov-erning equations for pre-buckling creep deformation and bifurcation buckling of laminated circular cylindrical shells under axial compression are obtained on the basis of the Donnell type shallow shell theory and Karman-Donnell geometrically nonlinear relationship. Corresponding solution strategy is constructed by inte-grating finite-difference technique, trigonometric series expansion method and Taylor's numerical recursive scheme for convolution integration. The bifurcation creep buckling of symmetrically laminated glass-epoxy circular cylindrical shells with matrix creep cracking coupled are examined for various geometrical parame-ters and parameters of damage evolution as well as boundary conditions. The nu-merical results show that matrix creep cracking remarkably shortens the critic time of bifurcation buckling and reduces the durable critic loads, and its effects become weak and finally vanish with the increase of the ratio of radius to thickness in the case of short laminated circular cylindrical shells, also the influence of the matrix creep cracking is mainly dependent on the boundary conditions at two ends for moderately long circular cylindrical shells.

Effect of matrix cracking on the time delayed buckling of viscoelastic laminated circular cylindrical shells

Institute of Scientific and Technical Information of China (English)

2008-01-01

The effect of matrix cracking on the bifurcation creep buckling of viscoelastic laminated circular cylindrical shells is investigated.The viscoelastic behavior of laminas is modeled by Schapery’s integral constitutive equation with growing matrix cracks.The values of damage variables are correlated to non-dimensional density of matrix cracks relying on the formulas from mesomechanics approach,and the evolution equation predicting the growth rate of density of matrix cracks is assumed to follow a power type relation with transverse tensile stress.The governing equations for prebuckling creep deformation and bifurcation buckling of laminated circular cylindrical shells under axial compression are obtained on the basis of the Donnell type shallow shell theory and Kármán-Donnell geometrically nonlinear relationship.Corresponding solution strategy is constructed by integrating finite-difference technique,trigonometric series expansion method and Taylor’s numerical recursive scheme for convolution integration.The bifurcation creep buckling of symmetrically laminated glass-epoxy circular cylindrical shells with matrix creep cracking coupled are examined for various geometrical parameters and parameters of damage evolution as well as boundary conditions.The numerical results show that matrix creep cracking remarkably shortens the critic time of bifurcation buckling and reduces the durable critic loads,and its effects become weak and finally vanish with the increase of the ratio of radius to thickness in the case of short laminated circular cylindrical shells,also the influence of the matrix creep cracking is mainly dependent on the boundary conditions at two ends for moderately long circular cylindrical shells.

Modeling of a partially debonded piezoelectric actuator in smart composite laminates

International Nuclear Information System (INIS)

Huang, Bin; Soo Kim, Heung; Ho Yoon, Gil

2015-01-01

A partially debonded piezoelectric actuator in smart composite laminates was modeled using an improved layerwise displacement field and Heaviside unit step functions. The finite element method with four node plate element and the extended Hamilton principle were used to derive the governing equation. The effects of actuator debonding on the smart composite laminate were investigated in both the frequency and time domains. The frequency and transient responses were obtained using the mode superposition method and the Newmark time integration algorithm, respectively. Two partial actuator debonding cases were studied to investigate the debonding effects on the actuation capability of the piezoelectric actuator. The effect of actuator debonding on the natural frequencies was subtler, but severe reductions of the actuation ability were observed in both the frequency and time responses, especially in the edge debonded actuator case. The results provided confirmation that the proposed modeling could be used in virtual experiments of actuator failure in smart composite laminates. (paper)

Performance analysis of smart laminated composite plate integrated with distributed AFC material undergoing geometrically nonlinear transient vibrations

Science.gov (United States)

Shivakumar, J.; Ashok, M. H.; Khadakbhavi, Vishwanath; Pujari, Sanjay; Nandurkar, Santosh

2018-02-01

The present work focuses on geometrically nonlinear transient analysis of laminated smart composite plates integrated with the patches of Active fiber composites (AFC) using Active constrained layer damping (ACLD) as the distributed actuators. The analysis has been carried out using generalised energy based finite element model. The coupled electromechanical finite element model is derived using Von Karman type nonlinear strain displacement relations and a first-order shear deformation theory (FSDT). Eight-node iso-parametric serendipity elements are used for discretization of the overall plate integrated with AFC patch material. The viscoelastic constrained layer is modelled using GHM method. The numerical results shows the improvement in the active damping characteristics of the laminated composite plates over the passive damping for suppressing the geometrically nonlinear transient vibrations of laminated composite plates with AFC as patch material.

The modelling and control of failure in bi-material ceramic laminates

International Nuclear Information System (INIS)

Phillipps, A.J.; Howard, S.J.; Clegg, W.J.; Clyne, T.W.

1993-01-01

Recent experimental and theoretical work on simple, single phase, laminated systems has indicated that failure resistant ceramics can be produced using an elegant method that avoids many of the problems and limitations of comparable fibrous ceramic composites. Theoretical work on these laminated systems has shown good agreement with experiment and simulated the effects of material properties and laminate structure on the composite performance. This work has provided guidelines for optimised laminate performance. In the current study, theoretical work has been simply extended to predict the behaviour of bi-material laminates with alternating layers of weak and strong material with different stiffnesses. Expressions for the strain energy release rates of internal advancing cracks are derived and combined with existing criteria to predict the failure behaviour of these laminates during bending. The modelling indicates three modes of failure dictated by the relative proportions, thicknesses and interfacial properties of the weak and strong phases. A critical percentage of strong phase is necessary to improve failure behaviour, in an identical argument to that for fibre composites. Incorporation of compliant layers is also investigated and implications for laminate design discussed. (orig.)

Irradiating rubber laminate containing sensitive agent in layer

International Nuclear Information System (INIS)

Bohm, G.G.A.

1979-01-01

Rubber compounds may be sensitized to cure or partially cure when subjected to irradiation. If certain layers of a rubber laminate are treated with sensitizing materials and other layers wth densensitizing materials a composite is produced having cured and uncured layers after it has been subjected to irradiation. This technique enables the production of composite laminates in which predetermined layers have predetermined physical characteristics which are desirable in the manufacture of the ultimate end product, for example, automotive tires. Several chemicals have been found to accelerate the cure of rubber compounds by irradiation and several have been found which inhibit the cure. Paradichlorobenzene (PDCB) and certain of the thioetherpolythiols are effective cure promotors. Effective retarders include aromatic oils, sulfur, sulfur cure accelerators and some rubber antioxidants and/or antiozonants of the substituted diphenylamine type. The dosage of irradiation is dependent upon several variables: the type of rubber, the promotor or retarder utilized, the level of the promotor or retarder, the thickness of the layer of material, the thickness of adjacent layers of materials, the sequence of the layers of material, the number of the layers of material and whether the irradiation is applied to one or both sides of the composite strip. The dosage may be controlled by the amount of energy employed so that the electrons do not completely penetrate the entire strip. This results in the irradiaton of part of the strip, but not the entire strip. (LL)

SUN2 Modulates HIV-1 Infection and Latency through Association with Lamin A/C To Maintain the Repressive Chromatin.

Science.gov (United States)

Sun, Wei-Wei; Jiao, Shi; Sun, Li; Zhou, Zhaocai; Jin, Xia; Wang, Jian-Hua

2018-05-01

The postintegrational latency of HIV-1 is characterized by reversible silencing of long terminal repeat (LTR)-driven transcription of the HIV genome. It is known that the formation of repressive chromatin at the 5'-LTR of HIV-1 proviral DNA impedes viral transcription by blocking the recruitment of positive transcription factors. How the repressive chromatin is formed and modulated during HIV-1 infection remains elusive. Elucidation of which chromatin reassembly factor mediates the reorganization of chromatin is likely to facilitate the understanding of the host's modulation of HIV-1 transcription and latency. Here we revealed that "Sad1 and UNC84 domain containing 2" (SUN2), an inner nuclear membrane protein, maintained the repressive chromatin and inhibited HIV LTR-driven transcription of proviral DNA through an association with lamin A/C. Specifically, lamin A/C tethered SUN2 to the nucleosomes 1 and 2 of the HIV-1 5'-LTR to block the initiation and elongation of HIV-1 transcription. SUN2 knockdown converted chromatin to an active form and thus enhanced the phosphorylation of RNA polymerase II and its recruitment to the 5'-LTR HIV-1 proviral DNA, leading to reactivation of HIV-1 from latency. Conversely, the exogenous factors such as tumor necrosis factor alpha (TNF-α) induced reactivation, and the replication of HIV-1 led to the disassociation between SUN2 and lamin A/C, suggesting that disruption of the association between SUN2 and lamin A/C to convert the repressive chromatin to the active form might be a prerequisite for the initiation of HIV-1 transcription and replication. Together, our findings indicate that SUN2 is a novel chromatin reassembly factor that helps to maintain chromatin in a repressive state and consequently inhibits HIV-1 transcription. IMPORTANCE Despite the successful use of scores of antiretroviral drugs, HIV latency poses a major impediment to virus eradication. Elucidation of the mechanism of latency facilitates the discovery of new

Building method for nuclear power plant

International Nuclear Information System (INIS)

Iba, Tsutomu.

1993-01-01

Outer shielding buildings and inner concretes are dismantled and removed. In this case, foundations are left as they are. Then, new outer shielding buildings and inner concretes are constructed on the old foundations by way of rubber a laminates. The outer shielding buildings and the inner concretes supported by the rubber laminates have an earthquake-proof structure and vibrations upon occurrence of earthquakes can be moderated by the rubber laminates. Therefore, it is not required to make the outer shielding buildings so strong, and the weight of them can be reduced. Accordingly, the weight of the buildings is reduced compared with the buildings before dismantling, so that they can be sufficiently supported by the old foundations. This enables to build a nuclear power plant without ensuring new construction sites. (I.N.)

Process-induced viscoelastic stress in composite laminates

International Nuclear Information System (INIS)

Stango, R.J.

1985-01-01

In recent years, considerable interest has developed in evaluating the stress response of composite laminates which is associated with cooling the material system from the cure temperature to room temperature. This research examines the fundamental nature of time-dependent residual-thermal stresses in composite laminates which are caused by the extreme temperature reduction encountered during the fabrication process. Viscoelastic stress in finite-width, symmetric composite laminates is examined on the basis of a formulation that employs an incremental hereditary integral approach in conjunction with a quasi-three dimensional finite element analysis. A consistent methodology is developed and employed for the characterization of lamina material properties. Special attention is given to the time-dependent stress response at ply-interface locations near the free-edge. In addition, the influence of cooling path on stress history is examined. Recently published material property data for graphite-epoxy lamina is employed in the analysis. Results of the investigation generally indicate that nominal differences between the thermoelastic and viscoelastic solutions are obtained. Slight changes of the final stress state are observed to result when different cooling paths are selected for the temperature history. The methodology employed is demonstrated to result in an accurate, efficient, and consistent approach for the viscoelastic analysis of advanced composite laminates

Estimation of piezoelastic and viscoelastic properties in laminated structures

DEFF Research Database (Denmark)

Araujo, A. L.; Soares, C. M. Mota; Herskovits, J.

2009-01-01

An inverse method for material parameter estimation of elastic, piezoelectric and viscoelastic laminated plate structures is presented. The method uses a gradient based optimization technique in order to solve the inverse problem, through minimization of an error functional which expresses...... the difference between experimental free vibration data and corresponding numerical data produced by a finite element model. The complex modulus approach is used to model the viscoelastic material behavior, assuming hysteretic type damping. Applications that illustrate the influence of adhesive material...

Hole-thru-laminate mounting supports for photovoltaic modules

Science.gov (United States)

Wexler, Jason; Botkin, Jonathan; Culligan, Matthew; Detrick, Adam

2015-02-17

A mounting support for a photovoltaic module is described. The mounting support includes a pedestal having a surface adaptable to receive a flat side of a photovoltaic module laminate. A hole is disposed in the pedestal, the hole adaptable to receive a bolt or a pin used to couple the pedestal to the flat side of the photovoltaic module laminate.

Flexural strengthening of Reinforced Concrete (RC) Beams Retrofitted with Corrugated Glass Fiber Reinforced Polymer (GFRP) Laminates

Science.gov (United States)

Aravind, N.; Samanta, Amiya K.; Roy, Dilip Kr. Singha; Thanikal, Joseph V.

2015-01-01

Strengthening the structural members of old buildings using advanced materials is a contemporary research in the field of repairs and rehabilitation. Many researchers used plain Glass Fiber Reinforced Polymer (GFRP) sheets for strengthening Reinforced Concrete (RC) beams. In this research work, rectangular corrugated GFRP laminates were used for strengthening RC beams to achieve higher flexural strength and load carrying capacity. Type and dimensions of corrugated profile were selected based on preliminary study using ANSYS software. A total of twenty one beams were tested to study the load carrying capacity of control specimens and beams strengthened with plain sheets and corrugated laminates using epoxy resin. This paper presents the experimental and theoretical study on flexural strengthening of Reinforced Concrete (RC) beams using corrugated GFRP laminates and the results are compared. Mathematical models were developed based on the experimental data and then the models were validated.

Hybrid Laminates for Application in North Conditions

Science.gov (United States)

Antipov, V. V.; Oreshko, E. I.; Erasov, V. S.; Serebrennikova, N. Yu.

2016-11-01

A hybrid aluminum-lithium alloy/SIAL laminate as a possible material for application in structures operated in North conditions is considered. The finite-element method is used for a buckling stability analysis of hybrid panels, bars, and plates. A technique allowing one to compare the buckling stability of multilayered hybrid plates is offered. Compression tests were run on a hybrid laminate wing panel as a prototype of the top panel of TU-204SM airplane made from a high-strength B95T2 aluminum alloy. It turned out that the lighter composite panel had a higher load-carrying capacity than the aluminum one. Results of investigation into the properties the hybrid aluminum-lithium alloy/SIAL laminate and an analysis of scientific-technical data on this subject showed that this composite material could be used in the elements of airframes, including those operated in north conditions.

Photovoltaic module and laminate

Science.gov (United States)

Bunea, Gabriela E.; Kim, Sung Dug; Kavulak, David F.J.

2018-04-10

A photovoltaic module is disclosed. The photovoltaic module has a first side directed toward the sun during normal operation and a second, lower side. The photovoltaic module comprises a perimeter frame and a photovoltaic laminate at least partially enclosed by and supported by the perimeter frame. The photovoltaic laminate comprises a transparent cover layer positioned toward the first side of the photovoltaic module, an upper encapsulant layer beneath and adhering to the cover layer, a plurality of photovoltaic solar cells beneath the upper encapsulant layer, the photovoltaic solar cells electrically interconnected, a lower encapsulant layer beneath the plurality of photovoltaic solar cells, the upper and lower encapsulant layers enclosing the plurality of photovoltaic solar cells, and a homogenous rear environmental protection layer, the rear environmental protection layer adhering to the lower encapsulant layer, the rear environmental protection layer exposed to the ambient environment on the second side of the photovoltaic module.

Type QH-200 conveyor-belt nuclear scale

International Nuclear Information System (INIS)

Li Shuming; Dong Shiyuan; Li Furong; Wan Zemin

1991-01-01

Type QH-200 conveyor-belt nuclear scale is a reliable and on-line device that weighs solids mass flow continuously by γ-ray absorption and scattering. The operation principle and measuring method are described. And some factors influencing the accuracy are discussed. The full scale total weight of type QH-200 nuclear scale is 10 6 t. The result of calibration in the industrial field shows that the accuracy of QH-200 is better than ±1.0% for typical belt loading

Arteriographical and pathological changes in chronic laminitis in dairy cattle.

Science.gov (United States)

Boosman, R; Nemeth, F; Gruys, E; Klarenbeek, A

1989-07-01

The arteriographic appearance of 76 bovine hind digits, obtained from a slaughterhouse, was related to the macroscopic signs of chronic laminitis in the digits. There were statistically significant correlations between the macroscopic and the arteriographic appearance of the claws. Subsequent histological examination of the radiographically abnormal arteries revealed features indicative of arteriosclerosis. The results of this study indicate that chronic laminitis develops following a subclinical attack of laminitis due to a continous hypoperfusion of the digit.

Local and global nonlinear dynamics of a parametrically excited rectangular symmetric cross-ply laminated composite plate

International Nuclear Information System (INIS)

Ye Min; Lu Jing; Zhang Wei; Ding Qian

2005-01-01

The present investigation deals with nonlinear dynamic behavior of a parametrically excited simply supported rectangular symmetric cross-ply laminated composite thin plate for the first time. The governing equation of motion for rectangular symmetric cross-ply laminated composite thin plate is derived by using von Karman equation. The geometric nonlinearity and nonlinear damping are included in the governing equations of motion. The Galerkin approach is used to obtain a two-degree-of-freedom nonlinear system under parametric excitation. The method of multiple scales is utilized to transform the second-order non-autonomous differential equations to the first-order averaged equations. Using numerical method, the averaged equations are analyzed to obtain the steady state bifurcation responses. The analysis of stability for steady state bifurcation responses in laminated composite thin plate is also given. Under certain conditions laminated composite thin plate may have two or multiple steady state bifurcation solutions. Jumping phenomenon occurs in the steady state bifurcation solutions. The chaotic motions of rectangular symmetric cross-ply laminated composite thin plate are also found by using numerical simulation. The results obtained here demonstrate that the periodic, quasi-periodic and chaotic motions coexist for a parametrically excited fore-edge simply supported rectangular symmetric cross-ply laminated composite thin plate under certain conditions

Bayesian inference model for fatigue life of laminated composites

DEFF Research Database (Denmark)

Dimitrov, Nikolay Krasimirov; Kiureghian, Armen Der; Berggreen, Christian

2016-01-01

A probabilistic model for estimating the fatigue life of laminated composite plates is developed. The model is based on lamina-level input data, making it possible to predict fatigue properties for a wide range of laminate configurations. Model parameters are estimated by Bayesian inference. The ...

Standard test method for translaminar fracture toughness of laminated and pultruded polymer matrix composite materials

CERN Document Server

American Society for Testing and Materials. Philadelphia

2004-01-01

1.1 This test method covers the determination of translaminar fracture toughness, KTL, for laminated and pultruded polymer matrix composite materials of various ply orientations using test results from monotonically loaded notched specimens. 1.2 This test method is applicable to room temperature laboratory air environments. 1.3 Composite materials that can be tested by this test method are not limited by thickness or by type of polymer matrix or fiber, provided that the specimen sizes and the test results meet the requirements of this test method. This test method was developed primarily from test results of various carbon fiber – epoxy matrix laminates and from additional results of glass fiber – epoxy matrix, glass fiber-polyester matrix pultrusions and carbon fiber – bismaleimide matrix laminates (1-4, 6, 7). 1.4 A range of eccentrically loaded, single-edge-notch tension, ESE(T), specimen sizes with proportional planar dimensions is provided, but planar size may be variable and adjusted, with asso...

Lateral testing of glued laminated timber tudor arch

Science.gov (United States)

Douglas R. Rammer; Philip Line

2016-01-01

Glued laminated timber Tudor arches have been in wide use in the United States since the 1930s, but detailed knowledge related to seismic design in modern U.S. building codes is lacking. FEMA P-695 (P-695) is a methodology to determine seismic performance factors for a seismic force resisting system. A limited P-695 study for glued laminated timber arch structures...

Lamination and end plate design studies of SSC Low Energy Booster magnet prototypes

International Nuclear Information System (INIS)

Li, N.

1993-01-01

The LEB machine includes six kinds of laminated magnets and 4 kinds of laminations. The main quadrupole magnet and low field and high field corrector quadrupoles use the same lamination shape. The chromaticity sextupole, corrector dipole, and main dipole have different lamination designs. To test the physical design and production procedure for the magnets, it is necessary to build 2 or 3 prototypes for each kind of magnet. The ZVI plant in Moscow, manufactured all 4 kinds of lamination punching dies for the LEB magnets. Each die takes 3 to 5 months to fabricate. SSCL manufactured laser cut laminated magnet prototypes in the SSC shop at the same time. Since the LEB cycles at 10 Hz, the high frequency current and laminated end plate design causes a delamination problem on the magnet end. This problem is of concern and will be addressed

Analysis of interlaminar stresses in symmetric and unsymmetric laminates under various loadings

Science.gov (United States)

Leger, C. A.; Chan, W. S.

1993-04-01

A quasi-three-dimensional finite-element model is developed to investigate the interlaminar stresses in a composite laminate under combined loadings. An isoparametric quadrilateral element with eight nodes and three degrees of freedom per node is the finite element used in this study. The element is used to model a composite laminate cross section loaded by tension, torsion, transverse shear, and both beam and chord bending which are representative of loading in a helicopter rotor system. Symmetric and unsymmetric laminates are examined with comparisons made between the interlaminar stress distributions and magnitudes for each laminate. Unsymmetric results are compared favorably to limited results found in literature. The unsymmetric interlaminar normal stress distribution in a symmetric laminate containing a free edge delamination is also examined.

Analysis of Thermo-Acoustic Emission from Damage in Composite Laminates under Thermal Cyclic Loading

International Nuclear Information System (INIS)

Kim, Young Bok; Min, Dae Hong; Lee, Deok Bo; Choi, Nak Sam

2001-01-01

An investigation on nondestructive evaluation of thermal stress-reduced damage in the composite laminates (3mm in thickness and [+45 6 /-45 6 ] S lay-up angles) has been performed using the thermo-acoustic emission technique. Reduction of thermo-AE events due to repetitive thermal load cycles showed a Kaiser effect. An analysis of the thermo-AE behavior determined the stress free temperature of composite laminates. Fiber fracture and matrix cracks were observed using the optical microscopy, scanning electron microscopy and ultrasonic C-sean. Short-Time Fourier Transform of thermo-AE signals offered the time-frequency characteristics which might classify the thermo-AE as three different types to estimate the damage processes of the composites

Climbing ripple structure and associated storm-lamination from a ...

Indian Academy of Sciences (India)

Pranhita–Godavari Valley, south India, displays well developed climbing ripple lamination and ... sedimentary environments, such as river flood .... Sediment, sequence and facies ..... tic Archaean Witwatersrand Supergroup, South Africa;.

NiCoCrAl/YSZ laminate composites fabricated by EB-PVD

International Nuclear Information System (INIS)

Shi Guodong; Wang Zhi; Liang Jun; Wu Zhanjun

2011-01-01

Highlights: → The metal-ceramic laminate composites were fabricated by EB-PVD. → Both metal and ceramic layers consisted of straight columns with banded structures. → Columnar grain size was limited by the periodic layer interfaces in the laminates. → Effect of columns on fracture property was decreased by limiting layer thickness. → Laminates showed greater specific strength than monolithic metal foil. - Abstract: Two NiCoCrAl/YSZ laminate composites (A and B) with different metal-layer thickness (∼35 μm and 14 μm, respectively) were fabricated by electron beam physical vapor deposition (EB-PVD). Their microstructure was examined and their mechanical properties were compared with the 289 μm thick NiCoCrAl monolithic foil produced by EB-PVD. Both the YSZ and NiCoCrAl layers of the laminate composites had columnar grain structure. But the periodic layer interfaces limited the columnar grain size. Some pores between the columns were also observed. It was found that the strength of the laminate A was equal approximately to that of the NiCoCrAl monolithic foil, and that laminate B had the greater strength. Moreover, the density of the foils decreased with the increasing thickness ratio of YSZ/NiCoCrAl layers and the increasing the layer number. Thus, comparing with the NiCoCrAl monolithic foil, the NiCoCrAl/YSZ laminate composites not only had the equal or greater strength, but also had the much greater specific strength.

Steel skin - SMC laminate structures for lightweight automotive manufacturing

Science.gov (United States)

Quagliato, Luca; Jang, Changsoon; Murugesan, Mohanraj; Kim, Naksoo

2017-09-01

In the present research work an innovative material, made of steel skin and sheet molding compound core, is presented and is aimed to be utilized for the production of automotive body frames. For a precise description of the laminate structure, the material properties of all the components, including the adhesive utilized as an interlayer, have been carried out, along with the simple tension test of the composite material. The result have shown that the proposed laminate structure has a specific yield strength 114% higher than 6061 T6 aluminum, 34% higher than 7075 T6 aluminum, 186% higher than AISI 304 stainless steel (30HRC) and 42% than SK5 high-strength steel (52HRC), showing its reliability and convenience for the realization of automotive components. After calibrating the material properties of the laminate structure, and utilizing as reference the simple tension results of the laminate structure, the derived material properties have been utilized for the simulation of the mechanical behavior of an automotive B-pillar. The results have been compared with those of a standard B-pillar made of steel, showing that the MS-SMC laminate structure manifests load and impact carry capacity comparable with those of high strength steel, while granting, at least, an 11% weight reduction.

Modeling the kinematics of multi-axial composite laminates as a stacking of 2D TIF plies

Science.gov (United States)

Ibañez, Ruben; Abisset-Chavanne, Emmanuelle; Chinesta, Francisco; Huerta, Antonio

2016-10-01

Thermoplastic composites are widely considered in structural parts. In this paper attention is paid to sheet forming of continuous fiber laminates. In the case of unidirectional prepregs, the ply constitutive equation is modeled as a transversally isotropic fluid, that must satisfy both the fiber inextensibility as well as the fluid incompressibility. When the stacking sequence involves plies with different orientations the kinematics of each ply during the laminate deformation varies significantly through the composite thickness. In our former works we considered two different approaches when simulating the squeeze flow induced by the laminate compression, the first based on a penalty formulation and the second one based on the use of Lagrange multipliers. In the present work we propose an alternative approach that consists in modeling each ply involved in the laminate as a transversally isotropic fluid - TIF - that becomes 2D as soon as incompressibility constraint and plane stress assumption are taken into account. Thus, composites laminates can be analyzed as a stacking of 2D TIF models that could eventually interact by using adequate friction laws at the inter-ply interfaces.

Nuclear substructure reorganization during late stageerythropoiesis is selective and does not involve caspase cleavage ofmajor nuclear substructural proteins

Energy Technology Data Exchange (ETDEWEB)

Krauss, Sharon Wald; Lo, Annie J.; Short, Sarah A.; Koury, MarkJ.; Mohandas, Narla; Chasis, Joel Anne

2005-04-06

Enucleation, a rare feature of mammalian differentiation, occurs in three cell types: erythroblasts, lens epithelium and keratinocytes. Previous investigations suggest that caspase activation functions in lens epithelial and keratinocyte enucleation, as well as in early erythropoiesis encompassing BFU-E differentiation to proerythroblast. To determine whether caspase activation contributes to later erythropoiesis and whether nuclear substructures other than chromatin reorganize, we analyzed distributions of nuclear subcompartment proteins and assayed for caspase-induced cleavage of subcompartmental target proteins in mouse erythroblasts. We found that patterns of lamin B in the filamentous network interacting with both the nuclear envelope and DNA, nuclear matrix protein NuMA, and splicing factors Sm and SC35 persisted during nuclear condensation, consistent with effective transcription of genes expressed late in differentiation. Thus nuclear reorganization prior to enucleation is selective, allowing maintenance of critical transcriptional processes independent of extensive chromosomal reorganization. Consistent with these data, we found no evidence for caspase-induced cleavage of major nuclear subcompartment proteins during late erythropoiesis, in contrast to what has been observed in early erythropoiesis and in lens epithelial and keratinocyte differentiation. These findings imply that nuclear condensation and extrusion during terminal erythroid differentiation involve novel mechanisms that do not entail major activation of apoptotic machinery.

Systematic Analysis of the Functional Relevance of Nuclear Structure and Mechanics in Breast Cancer Progression

Science.gov (United States)

2014-07-01

accepted 12 June 2014) Associate Editor David Schaffer oversaw the review of this article. Abstract—Cell motility plays a critical role in many...Adam SA, Shumaker DK, Kinjo M, Cremer T, Goldman RD (2008) The A- and B-type nuclear lamin networks: micro- domains involved in chromatin organization...Irwin A. Eydelnant¶, Don E. Olins, Ada L. Olins, Harald Herrmann**, David A. Weitz¶, and Jan Lammerding§‡‡ From the ‡Department of Integrative Biology

A genetic algorithm for the optimization of fiber angles in composite laminates

International Nuclear Information System (INIS)

Hwang, Shun Fa; Hsu, Ya Chu; Chen, Yuder

2014-01-01

A genetic algorithm for the optimization of composite laminates is proposed in this work. The well-known roulette selection criterion, one-point crossover operator, and uniform mutation operator are used in this genetic algorithm to create the next population. To improve the hill-climbing capability of the algorithm, adaptive mechanisms designed to adjust the probabilities of the crossover and mutation operators are included, and the elite strategy is enforced to ensure the quality of the optimum solution. The proposed algorithm includes a new operator called the elite comparison, which compares and uses the differences in the design variables of the two best solutions to find possible combinations. This genetic algorithm is tested in four optimization problems of composite laminates. Specifically, the effect of the elite comparison operator is evaluated. Results indicate that the elite comparison operator significantly accelerates the convergence of the algorithm, which thus becomes a good candidate for the optimization of composite laminates.

Separated type nuclear superheating reactor

International Nuclear Information System (INIS)

Hida, Kazuki.

1993-01-01

In a separated type nuclear superheating reactor, fuel assemblies used in a reactor core comprise fuel rods made of nuclear fuel materials and moderator rods made of solid moderating materials such as hydrogenated zirconium. Since the moderating rods are fixed or made detachable, high energy neutrons generated from the fuel rods are moderated by the moderating rods to promote fission reaction of the fuel rods. Saturated steams supplied from the BWR type reactor by the fission energy are converted to high temperature superheated steams while passing through a steam channel disposed between the fuel rods and the moderating rods and supplied to a turbine. Since water is not used but solid moderating materials sealed in a cladding tube are used as moderation materials, isolation between superheated steams and water as moderators is not necessary. Further, since leakage of heat is reduced to improve a heat efficiency, the structure of the reactor core is simplified and fuel exchange is facilitated. (N.H.)

Field performance of stress-laminated timber bridges on low-volume roads

Science.gov (United States)

M. A. Ritter; J. P. Wacker; S. R. Duwadi

1995-01-01

Stress-laminated timber bridges were first introduced in the United States in the late 1980s. Since that time, the concept of stress-laminating has received a great deal of attention and hundreds of bridges have been built. Most of these bridges are located on rural low-volume roads. To evaluate the performance of stress-laminated bridges, the United States Department...

Field performance of timber bridges. 13, Mohawk Canal stress-laminated bridge

Science.gov (United States)

P. D. Hilbrich Lee; X. Lauderdale

The Mohawk Canal bridge was constructed in August 1994, just outside Roll, Arizona. It is a simple-span, double-lane, stress-laminated deck superstructure, approximately 6.4 m (21 ft) long and 10.4 m (34 ft) wide and constructed with Combination 16F-V3 Douglas Fir glued-laminated timber beam laminations. The performance of the bridge was monitored continuously for 2...

On Subsurface Crack Growth in Fibre Metal Laminate Materials

National Research Council Canada - National Science Library

Randall, Christian

2003-01-01

Fatigue crack growth in fibre metal laminates (FMLs) is significantly more complex than in monolithic materials due to the interaction of various physical mechanisms that govern the growth of cracks in laminates...

Effect of resin system on the mechanical properties and water absorption of kenaf fibre reinforced laminates

International Nuclear Information System (INIS)

Rassmann, S.; Paskaramoorthy, R.; Reid, R.G.

2011-01-01

The objective of this study is to compare the mechanical and water absorption properties of kenaf (Hibiscus cannabinus L.) fibre reinforced laminates made of three different resin systems. The use of different resin systems is considered so that potentially complex and expensive fibre treatments are avoided. The resin systems used include a polyester, a vinyl ester and an epoxy. Laminates of 15%, 22.5% and 30% fibre volume fraction were manufactured by resin transfer moulding. The laminates were tested for strength and modulus under tensile and flexural loading. Additionally, tests were carried out on laminates to determine the impact energy, impact strength and water absorption. The results revealed that properties were affected in markedly different ways by the resin system and the fibre volume fraction. Polyester laminates showed good modulus and impact properties, epoxy laminates displayed good strength values and vinyl ester laminates exhibited good water absorption characteristics. Scanning electron microscope studies show that epoxy laminates fail by fibre fracture, polyester laminates by fibre pull-out and vinyl ester laminates by a combination of the two. A comparison between kenaf and glass laminates revealed that the specific tensile and flexural moduli of both laminates are comparable at the volume fraction of 15%. However, glass laminates have much better specific properties than the kenaf laminates at high fibre volume fractions for all three resins used.

Modal analysis of pre and post impacted nano composite laminates

Directory of Open Access Journals (Sweden)

R. Velmurugan

Full Text Available Modal analysis is carried out on pre and post impacted nano composite laminates. The laminates are prepared using 3, 5 and 8 layers of 610gsm glass woven roving mats(WRM with epoxy resin and montmorillonite(MMT clay content is varied from 1% to 5%. Impulse hammer technique is used to find natural frequency and damping factor of laminates. Medium velocity impact tests are conducted by using a gas gun. The vibration responses of natural frequency and damping factor are obtained and are studied for laminates with all edges clamped boundary conditions. Results show considerable improvement in natural frequency and damping factor due to nano clay addition. It is also seen that the nano clay controls the delamination due to impact loading.

Computational Fatigue Life Analysis of Carbon Fiber Laminate

Science.gov (United States)

Shastry, Shrimukhi G.; Chandrashekara, C. V., Dr.

2018-02-01

In the present scenario, many traditional materials are being replaced by composite materials for its light weight and high strength properties. Industries like automotive industry, aerospace industry etc., are some of the examples which uses composite materials for most of its components. Replacing of components which are subjected to static load or impact load are less challenging compared to components which are subjected to dynamic loading. Replacing the components made up of composite materials demands many stages of parametric study. One such parametric study is the fatigue analysis of composite material. This paper focuses on the fatigue life analysis of the composite material by using computational techniques. A composite plate is considered for the study which has a hole at the center. The analysis is carried on (0°/90°/90°/90°/90°)s laminate sequence and (45°/-45°)2s laminate sequence by using a computer script. The life cycles for both the lay-up sequence are compared with each other. It is observed that, for the same material and geometry of the component, cross ply laminates show better fatigue life than that of angled ply laminates.

Modeling delamination of FRP laminates under low velocity impact

Science.gov (United States)

Jiang, Z.; Wen, H. M.; Ren, S. L.

2017-09-01

Fiber reinforced plastic laminates (FRP) have been increasingly used in various engineering such as aeronautics, astronautics, transportation, naval architecture and their impact response and failure are a major concern in academic community. A new numerical model is suggested for fiber reinforced plastic composites. The model considers that FRP laminates has been constituted by unidirectional laminated plates with adhesive layers. A modified adhesive layer damage model that considering strain rate effects is incorporated into the ABAQUS / EXPLICIT finite element program by the user-defined material subroutine VUMAT. It transpires that the present model predicted delamination is in good agreement with the experimental results for low velocity impact.

Modeling the Non-Linear Response of Fiber-Reinforced Laminates Using a Combined Damage/Plasticity Model

Science.gov (United States)

Schuecker, Clara; Davila, Carlos G.; Pettermann, Heinz E.

2008-01-01

The present work is concerned with modeling the non-linear response of fiber reinforced polymer laminates. Recent experimental data suggests that the non-linearity is not only caused by matrix cracking but also by matrix plasticity due to shear stresses. To capture the effects of those two mechanisms, a model combining a plasticity formulation with continuum damage has been developed to simulate the non-linear response of laminates under plane stress states. The model is used to compare the predicted behavior of various laminate lay-ups to experimental data from the literature by looking at the degradation of axial modulus and Poisson s ratio of the laminates. The influence of residual curing stresses and in-situ effect on the predicted response is also investigated. It is shown that predictions of the combined damage/plasticity model, in general, correlate well with the experimental data. The test data shows that there are two different mechanisms that can have opposite effects on the degradation of the laminate Poisson s ratio which is captured correctly by the damage/plasticity model. Residual curing stresses are found to have a minor influence on the predicted response for the cases considered here. Some open questions remain regarding the prediction of damage onset.

Photodegradation in ballistic laminates: Spectroscopy and lifetime extension

Energy Technology Data Exchange (ETDEWEB)

Renschler, C.L.; Stallard, B.R.; White, C.A.; Garcia, M.J.; Morse, H.E. [Sandia National Labs., Albuquerque, NM (United States). Properties of Organic Materials Dept.

1996-06-01

Several years ago, the Materials and Process Sciences Center (Org. 1800) was asked by Dept. 9613 to study the materials aging issues which had led to the loss of ballistic protection by Armored Tractor (AT) windshields and windows. The authors speculated that this loss of impact strength was due to photodegradation of the polycarbonate (PC) inboard ply. They developed a spectroscopic method to identify changes in the outboard surface of the PC, and showed that the changes in the surface which occurred upon natural aging in the field could be reproduced by exposing the laminates to a simulated solar flux. Based on these results, they recommended changes in the adhesive interlayers to filter out the ultraviolet (UV) light causing the aging problem. Working with the laminate vendor, PPG, they re-designed the laminates to implement these changes and block essentially all UV light from the inboard ply. The most recent phase of this work involved accelerated solar aging of laminates made with the new design to verify that photoaging effects have been blocked by the new materials. They report here the results of that study, and recommended follow-on work.

Parvoviral nuclear import: bypassing the host nuclear-transport machinery.

Science.gov (United States)

Cohen, Sarah; Behzad, Ali R; Carroll, Jeffrey B; Panté, Nelly

2006-11-01

The parvovirus Minute virus of mice (MVM) is a small DNA virus that replicates in the nucleus of its host cells. However, very little is known about the mechanisms underlying parvovirus' nuclear import. Recently, it was found that microinjection of MVM into the cytoplasm of Xenopus oocytes causes damage to the nuclear envelope (NE), suggesting that the nuclear-import mechanism of MVM involves disruption of the NE and import through the resulting breaks. Here, fluorescence microscopy and electron microscopy were used to examine the effect of MVM on host-cell nuclear structure during infection of mouse fibroblast cells. It was found that MVM caused dramatic changes in nuclear shape and morphology, alterations of nuclear lamin immunostaining and breaks in the NE of infected cells. Thus, it seems that the unusual nuclear-import mechanism observed in Xenopus oocytes is in fact used by MVM during infection of host cells.

Effect of Ultrasonic Versus Manual Cementation on the Fracture Strength of Resin Composite Laminates

NARCIS (Netherlands)

Ozcan, M.; Mese, A.

2009-01-01

This study evaluated the effect of conventional versus ultrasonic cementation techniques on the fracture strength of resin composite laminates. In addition, the failure modes were assessed. Window-type preparations I mm above the cemento-enamel junction were made on intact human maxillary central

Lamination of organic solar cells and organic light emitting devices: Models and experiments

International Nuclear Information System (INIS)

Oyewole, O. K.; Yu, D.; Du, J.; Asare, J.; Fashina, A.; Anye, V. C.; Zebaze Kana, M. G.; Soboyejo, W. O.

2015-01-01

In this paper, a combined experimental, computational, and analytical approach is used to provide new insights into the lamination of organic solar cells and light emitting devices at macro- and micro-scales. First, the effects of applied lamination force (on contact between the laminated layers) are studied. The crack driving forces associated with the interfacial cracks (at the bi-material interfaces) are estimated along with the critical interfacial crack driving forces associated with the separation of thin films, after layer transfer. The conditions for successful lamination are predicted using a combination of experiments and computational models. Guidelines are developed for the lamination of low-cost organic electronic structures

Thermo-mechanical response of rigid plastic laminates for greenhouse covering

Directory of Open Access Journals (Sweden)

Silvana Fuina

2016-09-01

Full Text Available Innovation in the field of protected crops represents an argument of great applied and theoretical research attention due to constantly evolving technologies and automation for higher quality flower and vegetable production and to the corresponding environmental and economic impact. The aim of this paper is to provide an analysis of some thermomechanical properties of rigid polymeric laminates for greenhouses claddings, including innovative tests such as the thermographic ones. Four types of laminates have been analysed: two polycarbonates, a polymethylmethacrylate and a polyethylene terephthalate (PET. The tests gave interesting results on different important properties, such as radiometric properties, limit stresses, strains and ductility. Moreover, a direct comparison of infrared images and force elongation curves gave important information on the relation of the (localised or homogeneous damage evolution, with both an applicative and theoretical implication. Finally, even if to the authors knowledge at present there are no examples of using PET for covering greenhouses, the results of this paper indicates the thermomechanical and radiometric characteristics of this material make it interesting for agricultural applications.

Optimization of the bamboo guadua angustifolia kunth in the elaboration of glued laminated elements for constructive use

International Nuclear Information System (INIS)

Díaz, G A; Cruz, R A; Chávez, A M

2013-01-01

Bamboo is considered one of the best timber resources in the world because for its mechanical properties and high sustainability; this research aims to improve the mechanical properties of the laminated glued bamboo Guadua Angustifolia Kunth (GAK) for use as structural elements, starting from de very manufacture process; this is important because it is possible to observe variations in the flexural strength and the elastic modulus in GAK samples taken from different heights and thickness of the culm. In order to analyze the influence of these final mechanical properties variations in the laminated, the height of the culm where samples are extracted (cepa, basa and sobrebasa) it is taken as a variable from where different types of laminated were manufactured, seeking to make optimal the configuration based in the transversal section area and the material strength. Three assemblies were designed varying the overlap of the adhesion lines and it concluded that the highest strength average values were obtained in the laminated composites manufactured with samples taken from the bottom of the culm (basa), which is possible because in these elements there are less adhesion lines than the other ones (middle, top and mixed) or the better matching of themselves

Predictions of Poisson's ratio in cross-ply laminates containing matrix cracks and delaminations

Science.gov (United States)

Harris, Charles E.; Allen, David H.; Nottorf, Eric W.

1989-01-01

A damage-dependent constitutive model for laminated composites has been developed for the combined damage modes of matrix cracks and delaminations. The model is based on the concept of continuum damage mechanics and uses second-order tensor valued internal state variables to represent each mode of damage. The internal state variables are defined as the local volume average of the relative crack face displacements. Since the local volume for delaminations is specified at the laminate level, the constitutive model takes the form of laminate analysis equations modified by the internal state variables. Model implementation is demonstrated for the laminate engineering modulus E(x) and Poisson's ratio nu(xy) of quasi-isotropic and cross-ply laminates. The model predictions are in close agreement to experimental results obtained for graphite/epoxy laminates.

Optimization of Laminated Composite Structures

DEFF Research Database (Denmark)

Henrichsen, Søren Randrup

of the contributions of the PhD project are included in the second part of the thesis. Paper A presents a framework for free material optimization where commercially available finite element analysis software is used as analysis tool. Robust buckling optimization of laminated composite structures by including...... allows for a higher degree of tailoring of the resulting material. To enable better utilization of the composite materials, optimum design procedures can be used to assist the engineer. This PhD thesis is focused on developing numerical methods for optimization of laminated composite structures...... nonlinear analysis of structures, buckling and post-buckling analysis of structures, and formulations for optimization of structures considering stiffness, buckling, and post-buckling criteria. Lastly, descriptions, main findings, and conclusions of the papers are presented. The papers forming the basis...

Influence of lamination processes on the behaviour of a pipe with a circumference crack

International Nuclear Information System (INIS)

Grebner, H.; Hoefler, A.; Hunger, H.

1994-01-01

A series of structural and mechanical fracturing follow-up calculations were carried out with respect to the PHDR-leakage test E22.06, which are supposed to take into account especially the temperature influences. The main emphasis was put on the analysis taking into consideration the lamination effects, as they were encountered in the experiment. On the one hand pre-supposed reasonable temperature distributions were used, on the other hand the attempt was made to deduct a lamination distribution for the whole test pipe from the temperature measurements. (orig.) [de

A Study on Dismantling of Westinghouse Type Nuclear Reactor

International Nuclear Information System (INIS)

Jeong, Woo-Tae; Lee, Sang-Guk

2014-01-01

KHNP started a research project this year to develop a methodology to dismantle nuclear reactors and internals. In this paper, we reviewed 3D design model of the reactor and suggested feasible cutting scheme.. Using 3-D CAD model of Westinghouse type nuclear reactor and its internals, we reviewed possible options for disposal. Among various options of dismantling the nuclear reactor, plasma cutting was selected to be the best feasible and economical method. The upper internals could be segmented by using a band saw. It is relatively fast, and easily maintained. For cutting the lower internals, plasma torch was chosen to be the best efficient tool. Disassembling the baffle and the former plate by removing the baffle former bolts was also recommended for minimizing storage volume. When using plasma torch for cutting the reactor vessel and its internal, installation of a ventilation system for preventing pollution of atmosphere was recommended. For minimizing radiation exposure during the cutting operation, remotely controlled robotic tool was recommended to be used

The role of shape memory alloy on impact response of glass/epoxy laminates under low temperature

International Nuclear Information System (INIS)

Kang, K. W.; Kim, H. J.

2007-01-01

The paper aims to evaluate the impact response of glass/epoxy laminates with embedded shape memory alloy (SMA) subject to low velocity impact at various temperatures. For the goal, the impact tests were performed by using an instrumented impact-testing machine at three temperatures: 293K, 263K and 233K for the baseline (laminates without SMA wires) and SMA laminates (laminates with embedded SMA wires). And the resultant damages were inspected through the scanning acoustic microscope (SAM). Also, based on the impact force history and the damage configuration, the impact resistance parameters were employed to evaluate damage resistance of laminates with embedded SMA wires. As a result, it was observed that the damage resistance of glass/epoxy laminates is influenced by embedded SMA wires and embedding SMA wires into laminates does not compromise the structure any differently to laminates without wires. In fact, it has been shown that under lower temperature, the SMA laminates have a little superior damage resistance compared with the baseline laminates

Feeding practices and potential risk factors for laminitis in dairy cows in Thailand

OpenAIRE

Pilachai, R.

2013-01-01

Laminitis is considered an important health problem facing the Thai dairy industry. Although the etiology of laminitis is multifactorial, nutrition is considered an important risk factor. Rumen acidosis, lipopolysaccharides (LPS) and histamine may play a role in the development of laminitis in dairy cattle. However, the relevancy of these risk factors in relation to the occurrence of laminitis under practical feeding conditions in Thailand is not clear. In Thailand, dairy rations are generall...

Inhibitory function of adapter-related protein complex 2 alpha 1 subunit in the process of nuclear translocation of human immunodeficiency virus type 1 genome

International Nuclear Information System (INIS)

Kitagawa, Yukiko; Kameoka, Masanori; Shoji-Kawata, Sanae; Iwabu, Yukie; Mizuta, Hiroyuki; Tokunaga, Kenzo; Fujino, Masato; Natori, Yukikazu; Yura, Yoshiaki; Ikuta, Kazuyoshi

2008-01-01

The transfection of human cells with siRNA against adapter-related protein complex 2 alpha 1 subunit (AP2α) was revealed to significantly up-regulate the replication of human immunodeficiency virus type 1 (HIV-1). This effect was confirmed by cell infection with vesicular stomatitis virus G protein-pseudotyped HIV-1 as well as CXCR4-tropic and CCR5-tropic HIV-1. Viral adsorption, viral entry and reverse transcription processes were not affected by cell transfection with siRNA against AP2α. In contrast, viral nuclear translocation as well as the integration process was significantly up-regulated in cells transfected with siRNA against AP2α. Confocal fluorescence microscopy revealed that a subpopulation of AP2α was not only localized in the cytoplasm but was also partly co-localized with lamin B, importin β and Nup153, implying that AP2α negatively regulates HIV-1 replication in the process of nuclear translocation of viral DNA in the cytoplasm or the perinuclear region. We propose that AP2α may be a novel target for disrupting HIV-1 replication in the early stage of the viral life cycle

Enhanced laminated composite phase change material for energy storage

Energy Technology Data Exchange (ETDEWEB)

Darkwa, J.; Zhou, T. [Centre for Sustainable Energy Technologies (CSET), The University of Nottingham Ningbo, 199 Taikang East Road, Ningbo 315100 (China)

2011-02-15

This paper summarises studies undertaken towards the development of a laminated composite aluminium/hexadecane phase change material (PCM) drywall based on previous analytical work. The study also covered the selection and testing of various types of adhesive materials and identified Polyvinyl acetate (PVA) material as a suitable bonding material. For the purpose of comparison pure hexadecane and composite aluminium/hexadecane samples were developed and tested. The test results revealed faster thermal response by the aluminium/hexadecane sample regarding the rate of heat flux and also achieved about 10% and 15% heat transfer enhancements during the charging and discharging periods respectively. Its measured effective thermal conductivity also increased remarkably to 1.25 W/mK as compared with 0.15 W/mK for pure hexadecane. However there was about 5% less total cumulative thermal energy discharged at the end of the test which indicates that its effective thermal capacity was reduced by the presence of the aluminium particles. The study has shown that some of the scientific and technical barriers associated with the development of laminated composite PCM drywall systems can be overcome but further investigations of effects of adhesive materials are needed. (author)

Eddy-current effect on resonant magnetoelectric coupling in magnetostrictive-piezoelectric laminated composites

Science.gov (United States)

Liu, Guoxi; Zhang, Chunli; Chen, Weiqiu; Dong, Shuxiang

2013-07-01

An analytical model of resonant magnetoelectric (ME) coupling in magnetostrictive (MS)-piezoelectric (PE) laminated composites in consideration of eddy-current effect in MS layer using equivalent circuit method is presented. Numerical calculations show that: (1) the eddy-current has a strong effect on ME coupling in MS-PE laminated composites at resonant frequency; and (2) the resonant ME coupling is then significantly dependent on the sizes of ME laminated composites, which were neglected in most previous theoretical analyses. The achieved results provide a theoretical guidance for the practice engineering design, manufacture, and application of ME laminated composites and devices.

Laser cutting of laminated sheet material: a modeling exercise

NARCIS (Netherlands)

de Graaf, R.F.; Meijer, J.

1997-01-01

Laser cutting has been investigated for a number of aluminum-synthetic laminates, newly developed materials for the aeronautic and automotive industry. The materials consist of alternating aluminum and synthetic layers. It is shown that these materials can be cut at rates comparable to those of

Retortable Laminate/Polymeric Food Tubes for Specialized Feeding

Science.gov (United States)

2012-06-01

Report STP #3010 Results and Accomplishments (June 2010 – June 2012) Report No: FTR 303 CDRL Sequence: A003 June 2012 CORANET CONTRACT #: SP4701-08-D...June 2010 - June 2012 Retortable Laminate/Polymeric Food Tubes for Specialized Feeding - STP # 3010 SP4701-08-D-0004 MANTECH (0708011S) CORANET A003...on commercial off-the-shelf materials and not military unique. A market survey of commercially available laminated tubes revealed that they are all

Feeding practices and potential risk factors for laminitis in dairy cows in Thailand

NARCIS (Netherlands)

Pilachai, R.

2013-01-01

Laminitis is considered an important health problem facing the Thai dairy industry. Although the etiology of laminitis is multifactorial, nutrition is considered an important risk factor. Rumen acidosis, lipopolysaccharides (LPS) and histamine may play a role in the development of laminitis in dairy

Numerical analysis of laminated elastomer by FEM

International Nuclear Information System (INIS)

Mazda, T.; Shiojiri, H.

1993-01-01

A Computer code based on mixed finite element method was developed for three dimensional large strain analyses of laminated elastomers including nonlinear bulk stress vs. bulk strain relationships. The adopted element is the variable node element with maximum node numbers of 27 for displacements and 4 for pressures. At first, the displacements and pressures were calculated by the code using single element under various loading conditions. The results were compared with theoretical solutions and the both results' exactly coincided with each other. Next, the analyses of laminated elastomers subjected to axial loadings were conducted using both the new code and ABAQUS code, and the results were compared with the test results. The agreement of the results of the present code were better than ABAQUS code mainly due to the capability of handling wider range of material properties. Lastly, the shearing tests of laminated elastomers were simulated by the new code. The results were shown to be in good agreement with the test results. (author)

Numerical Material Model for Composite Laminates in High-Velocity Impact Simulation

Directory of Open Access Journals (Sweden)

Tao Liu

Full Text Available Abstract A numerical material model for composite laminate, was developed and integrated into the nonlinear dynamic explicit finite element programs as a material user subroutine. This model coupling nonlinear state of equation (EOS, was a macro-mechanics model, which was used to simulate the major mechanical behaviors of composite laminate under high-velocity impact conditions. The basic theoretical framework of the developed material model was introduced. An inverse flyer plate simulation was conducted, which demonstrated the advantage of the developed model in characterizing the nonlinear shock response. The developed model and its implementation were validated through a classic ballistic impact issue, i.e. projectile impacting on Kevlar29/Phenolic laminate. The failure modes and ballistic limit velocity were analyzed, and a good agreement was achieved when comparing with the analytical and experimental results. The computational capacity of this model, for Kevlar/Epoxy laminates with different architectures, i.e. plain-woven and cross-plied laminates, was further evaluated and the residual velocity curves and damage cone were accurately predicted.

The role of endotoxin in the pathogenesis of acute bovine laminitis.

Science.gov (United States)

Boosman, R; Mutsaers, C W; Klarenbeek, A

1991-07-01

To study the possible role of endotoxin in the pathogenesis of bovine laminitis, local and systemic injections of endotoxin (E. coli 0111 B4) with different doses were given to three groups of four cows each. Clinical and haematologic parameters indicated an acute-phase response, including positive plasma ethanol gelation (soluble fibrin), the occurrence of fibrin degradation products and decreased thrombocyte counts. Local Shwartzman reactions were not evoked. Clinical examination of the claws and the gait of the animals revealed no signs of laminitis. However, on histopathological examination of the claw corium signs of laminitis such as vacuolisation of the Stratum basale, lymphocyte and leucocyte infiltration and thrombosis were found. These results indicate that endotoxin indeed may be involved in the pathogenesis of laminitis. For the development of a clinical acute laminitis model in cattle either another dosage, other toxins or factors in addition to the endotoxin used in this experiment are needed.

Tungsten foil laminate for structural divertor applications - Joining of tungsten foils

Science.gov (United States)

Reiser, Jens; Rieth, Michael; Möslang, Anton; Dafferner, Bernhard; Hoffmann, Jan; Mrotzek, Tobias; Hoffmann, Andreas; Armstrong, D. E. J.; Yi, Xiaoou

2013-05-01

This paper is the fourth in our series on tungsten laminates. The aim of this paper is to discuss laminate synthesis, meaning the joining of tungsten foils. It is obvious that the properties of the tungsten laminate strongly depend on the combination of (i) interlayer and (ii) joining technology, as this combination defines (i) the condition of the tungsten foil after joining (as-received or recrystallised) as well as (ii) the characteristics of the interface between the tungsten foil and the interlayer (wettability or diffusion leading to a solid solution or the formation of intermetallics). From the example of tungsten laminates joined by brazing with (i) an eutectic silver copper brazing filler, (ii) copper, (iii) titanium, and (iv) zirconium, the microstructure will be discussed, with special focus on the interface. Based on our assumptions of the mechanism of the extraordinary ductility of tungsten foil we present three syntheses strategies and make recommendations for the synthesis of high temperature tungsten laminates.

Feasibility of Electromagnetic Acoustic Evaluation for Quality Test of a Plate-type Nuclear Fuel

International Nuclear Information System (INIS)

Jung, Hyun Kyu; Lee, Yoon Sang; Cheong, Yong Moo

2010-01-01

Most research and test reactors use the nuclear fuel plates which are consisted of a fuel core in aluminum alloy. Recently KAERI signed a deal with the Jordan Atomic Energy Commission to build the research reactor and have to supply the plate-type nuclear fuels. For the demands of world market, KAERI started the research and development of the plate-type fuel elements and endeavored to achieve a localization of fuel fabrication. For the inspection of plate-type fuel elements to be used in Research Reactors, an immersion pulse-echo ultrasonic technique was applied. This inspection was done with water, so a nuclear fuel was immersed to be prone to corrosion and needed to have time and cost due to an additional process. The sample that will be examined within this paper is a non-ferromagnetic material such as aluminum which has a good acousto-elastic property, for an effective inspection of a bond quality for a nuclear fuel under a manufacturing environment. The purpose of this study is to investigate the feasibility of an EMAT technology for an automated inspection of a nuclear fuel without water

Development of a new dual phase steel with laminated microstructural morphology

Energy Technology Data Exchange (ETDEWEB)

Saeidi, N., E-mail: navidsae@gmail.com [Department of Materials Engineering, Isfahan University of Technology, Isfahan, 4156–83111 (Iran, Islamic Republic of); Karimi, M. [Department of Materials Science and Engineering, Shahrood University of Technology, Shahrood, 3619995161 (Iran, Islamic Republic of); Toroghinejad, M.R. [Department of Materials Engineering, Isfahan University of Technology, Isfahan, 4156–83111 (Iran, Islamic Republic of)

2017-05-01

The development of dual phase steels to meet the current world demands, for the purpose of decreasing the fuel consumption with increasing the strength to weight ratio, requires certain microstructural modifications. In the present research, a new morphology of DP steel, known as Laminated–DP steel, as well as its unique production method has been introduced. The new process developed involved properly selecting low carbon steels, stacking them in a laminated manner and performing a roll bonding process followed by short austenitization treatment. The martensite volume fraction was designed and obtained to be 24%. Scanning electron microscopy (SEM) was employed for microstructural examination. Moreover, deformation and tensile behavior of the newly developed steel were studied and compared with those of some ordinary DP steel (ODP). Room temperature uniaxial tensile tests also revealed mechanical properties comparable with those of the commercial DP600 steel, a kind of structural automotive steel. - Highlights: • A new method for producing dual phase steels was introduced. • Employing a new thermo-mechanical process a laminated microstructure was obtained. • Mechanical properties of the new laminated DP steel were studied. • Tensile properties of the new DP steel were comparable with those of the commercial DP600 steel.

A superconducting magnet mandrel with minimum symmetry laminations for proton therapy

Science.gov (United States)

Caspi, S.; Arbelaez, D.; Brouwer, L.; Dietderich, D. R.; Felice, H.; Hafalia, R.; Prestemon, S.; Robin, D.; Sun, C.; Wan, W.

2013-08-01

The size and weight of ion-beam cancer therapy gantries are frequently determined by a large aperture, curved, ninety degree, dipole magnet. The higher fields achievable with superconducting technology promise to greatly reduce the size and weight of this magnet and therefore also the gantry as a whole. This paper reports advances in the design of winding mandrels for curved, canted cosine-theta (CCT) magnets in the context of a preliminary magnet design for a proton gantry. The winding mandrel is integral to the CCT design and significantly affects the construction cost, stress management, winding feasibility, eddy current power losses, and field quality of the magnet. A laminated mandrel design using a minimum symmetry in the winding path is introduced and its feasibility demonstrated by a rapid prototype model. Piecewise construction of the mandrel using this laminated approach allows for increased manufacturing techniques and material choices. Sectioning the mandrel also reduces eddy currents produced during field changes accommodating the scan of beam energies during treatment. This symmetry concept can also greatly reduce the computational resources needed for 3D finite element calculations. It is shown that the small region of symmetry forming the laminations combined with periodic boundary conditions can model the entire magnet geometry disregarding the ends.

Binder-free highly conductive graphene laminate for low cost printed radio frequency applications

Energy Technology Data Exchange (ETDEWEB)

Huang, Xianjun; Leng, Ting; Zhang, Xiao; Hu, Zhirun, E-mail: Z.Hu@manchester.ac.uk [School of Electrical and Electronic Engineering, University of Manchester, Manchester (United Kingdom); Chen, Jia Cing; Chang, Kuo Hsin [BGT Materials Limited, Photon Science Institute, University of Manchester, Manchester M13 9PL (United Kingdom); Geim, Andre K. [Manchester Centre for Mesoscience and Nanotechnology, University of Manchester, Manchester (United Kingdom); Novoselov, Kostya S. [School of Physics and Astronomy, University of Manchester, Manchester (United Kingdom)

2015-05-18

In this paper, we demonstrate realization of printable radio frequency identification (RFID) antenna by low temperature processing of graphene ink. The required ultra-low resistance is achieved by rolling compression of binder-free graphene laminate. With compression, the conductivity of graphene laminate is increased by more than 50 times compared to that of as-deposited one. Graphene laminate with conductivity of 4.3 × 10{sup 4 }S/m and sheet resistance of 3.8 Ω/sq (with thickness of 6 μm) is presented. Moreover, the formation of graphene laminate from graphene ink reported here is simple and can be carried out in low temperature (100 °C), significantly reducing the fabrication costs. A dipole antenna based on the highly conductive graphene laminate is further patterned and printed on a normal paper to investigate its RF properties. The performance of the graphene laminate antenna is experimentally measured. The measurement results reveal that graphene laminate antenna can provide practically acceptable return loss, gain, bandwidth, and radiation patterns, making it ideal for low cost printed RF applications, such as RFID tags and wearable wireless sensor networks.

Binder-free highly conductive graphene laminate for low cost printed radio frequency applications

International Nuclear Information System (INIS)

Huang, Xianjun; Leng, Ting; Zhang, Xiao; Hu, Zhirun; Chen, Jia Cing; Chang, Kuo Hsin; Geim, Andre K.; Novoselov, Kostya S.

2015-01-01

In this paper, we demonstrate realization of printable radio frequency identification (RFID) antenna by low temperature processing of graphene ink. The required ultra-low resistance is achieved by rolling compression of binder-free graphene laminate. With compression, the conductivity of graphene laminate is increased by more than 50 times compared to that of as-deposited one. Graphene laminate with conductivity of 4.3 × 10 4  S/m and sheet resistance of 3.8 Ω/sq (with thickness of 6 μm) is presented. Moreover, the formation of graphene laminate from graphene ink reported here is simple and can be carried out in low temperature (100 °C), significantly reducing the fabrication costs. A dipole antenna based on the highly conductive graphene laminate is further patterned and printed on a normal paper to investigate its RF properties. The performance of the graphene laminate antenna is experimentally measured. The measurement results reveal that graphene laminate antenna can provide practically acceptable return loss, gain, bandwidth, and radiation patterns, making it ideal for low cost printed RF applications, such as RFID tags and wearable wireless sensor networks

Dowelled structural connections in laminated bamboo and timber

OpenAIRE

Reynolds, Thomas Peter; Sharma, Bhavna; Harries, Kent; Ramage, Michael Hector

2015-01-01

Structural sections of laminated bamboo can be connected using methods common in timber engineering, however the different material properties of timber and laminated bamboo suggest that the behaviour of connections in the two materials would not be the same. This study investigates the dowelled connection, in which a connector is passed through a hole in the material, and load is resisted by shear in the connector and embedment into the surrounding material. Steel dowels were used in a conne...

Evaluation of the effectiveness of lamination for preventing discoloration and fading of radiation warning signs posted on the exterior of radiation facilities

International Nuclear Information System (INIS)

Hiroi, Tomoko; Ootaki, Masanori; Nawa, Yukino; Kuwabara, Rie; Tatsunami, Shinobu; Matsui, Hiroaki; Kumazawa, Yutaka; Yamamoto, Takio

2014-01-01

Radiation warning signs posted on the exterior of radiation facilities become faded and discolored with time. There are various types of commercially available laminating films for protecting signs from ultraviolet light. We examined the protection effect of polyvinyl chloride (PVC), acrylic resin and fluororesin films applied to the surface of radiation warning signs. The laminated signs were exposed to direct sunlight on the wall of an air filter chamber of a radiation facility for 1200 days. Simultaneously, another set of laminated signs was exposed to light from a xenon-arc weatherometer for 1200 hours. After exposure, the colors on the surface of each sign were evaluated digitally by using a spectrum colorimeter. The results indicated that lamination with a film that blocks ultraviolet light is effective for protecting the signs from fading and discoloration. For long-term protection under direct sunlight, PVC was the most effective among the three materials tested. (author)

A summary of modulus of elasticity and knot size surveys for laminating grades of lumber

Science.gov (United States)

R. W. Wolfe; R. C. Moody

1981-01-01

A summary of modulus of elasticity (MOE) and knot data is presented for grades of lumber commonly used to manufacture glued-laminated (glulam) timber by the laminating Industry. Tabulated values represent 30 different studies covering a time span of over 16 years. Statistical estimates of average and near-maximum knot sizes as well as mean and coefficient of variation...

Free vibration of fully functionally graded carbon nanotube reinforced graphite/epoxy laminates

Science.gov (United States)

Kuo, Shih-Yao

2018-03-01

This study provides the first-known vibration analysis of fully functionally graded carbon nanotube reinforced hybrid composite (FFG-CNTRHC) laminates. CNTs are non-uniformly distributed to reinforce the graphite/epoxy laminates. Some CNT distribution functions in the plane and thickness directions are proposed to more efficiently increase the stiffening effect. The rule of mixtures is modified by considering the non-homogeneous material properties of FFG-CNTRHC laminates. The formulation of the location dependent stiffness matrix and mass matrix is derived. The effects of CNT volume fraction and distribution on the natural frequencies of FFG-CNTRHC laminates are discussed. The results reveal that the FFG layout may significantly increase the natural frequencies of FFG-CNTRHC laminate.

Laminated structure in internally oxidized Ru-Ta coatings

Energy Technology Data Exchange (ETDEWEB)

Chen, Yung-I, E-mail: yichen@mail.ntou.edu.tw

2012-12-01

During the development of refractory alloy coatings for protective purposes at high temperature under oxygen-containing atmospheres, previous studies noted and examined the internal oxidation phenomenon for Mo-Ru and Ru-Ta coatings. The internally oxidized zone shows a laminated structure, consisting of alternating oxygen-rich and deficient layers stacked with a general orientation. Previous studies proposed a forming mechanism. To investigate in detail, Ru-Ta coatings were prepared with various rotating speeds of a substrate-holder. The coatings were annealed at 600 Degree-Sign C in an atmosphere continuously purged with 1% O{sub 2}-99% Ar mixed gas for 30 min. Transmission electron microscopy was used to examine the laminated-layer periods. Auger electron spectroscopy depth profiles certified the periodical variation of the related constituents. X-ray photoelectron spectroscopy proved the valence variation of Ta in the near surface, accompanied by the introduction of oxygen ions. The inward diffusion of oxygen was dominated by lattice diffusion. - Highlights: Black-Right-Pointing-Pointer Laminated Ru-Ta coatings consisted of a cyclical gradient concentration. Black-Right-Pointing-Pointer The as-deposited coatings showed a laminated structure with a period of 4-34 nm. Black-Right-Pointing-Pointer Internal oxidation of Ru-Ta coatings executed after annealing in 1% O{sub 2}-Ar atmosphere. Black-Right-Pointing-Pointer Oxygen inward diffusion was dominated by lattice diffusion.

Soft magnetic characteristics of laminated magnetic block cores assembled with a high Bs nanocrystalline alloy

Directory of Open Access Journals (Sweden)

Atsushi Yao

2018-05-01

Full Text Available This paper focuses on an evaluation of core losses in laminated magnetic block cores assembled with a high Bs nanocrystalline alloy in high magnetic flux density region. To discuss the soft magnetic properties of the high Bs block cores, the comparison with amorphous (SA1 block cores is also performed. In the high Bs block core, both low core losses and high saturation flux densities Bs are satisfied in the low frequency region. Furthermore, in the laminated block core made of the high Bs alloy, the rate of increase of iron losses as a function of the magnetic flux density remains small up to around 1.6 T, which cannot be realized in conventional laminated block cores based on amorphous alloy. The block core made of the high Bs alloy exhibits comparable core loss with that of amorphous alloy core in the high-frequency region. Thus, it is expected that this laminated high Bs block core can achieve low core losses and high saturation flux densities in the high-frequency region.

Finite element modeling of small-scale tapered wood-laminated composite poles with biomimicry features

Science.gov (United States)

Cheng Piao; Todd F. Shupe; R.C. Tang; Chung Y. Hse

2008-01-01

Tapered composite poles with biomimicry features as in bamboo are a new generation of wood laminated composite poles that may some day be considered as an alternative to solid wood poles that are widely used in the transmission and telecommunication fields. Five finite element models were developed with ANSYS to predict and assess the performance of five types of...

Stochastic-Strength-Based Damage Simulation of Ceramic Matrix Composite Laminates

Science.gov (United States)

Nemeth, Noel N.; Mital, Subodh K.; Murthy, Pappu L. N.; Bednarcyk, Brett A.; Pineda, Evan J.; Bhatt, Ramakrishna T.; Arnold, Steven M.

2016-01-01

The Finite Element Analysis-Micromechanics Analysis Code/Ceramics Analysis and Reliability Evaluation of Structures (FEAMAC/CARES) program was used to characterize and predict the progressive damage response of silicon-carbide-fiber-reinforced reaction-bonded silicon nitride matrix (SiC/RBSN) composite laminate tensile specimens. Studied were unidirectional laminates [0] (sub 8), [10] (sub 8), [45] (sub 8), and [90] (sub 8); cross-ply laminates [0 (sub 2) divided by 90 (sub 2),]s; angled-ply laminates [plus 45 (sub 2) divided by -45 (sub 2), ]s; doubled-edge-notched [0] (sub 8), laminates; and central-hole laminates. Results correlated well with the experimental data. This work was performed as a validation and benchmarking exercise of the FEAMAC/CARES program. FEAMAC/CARES simulates stochastic-based discrete-event progressive damage of ceramic matrix composite and polymer matrix composite material structures. It couples three software programs: (1) the Micromechanics Analysis Code with Generalized Method of Cells (MAC/GMC), (2) the Ceramics Analysis and Reliability Evaluation of Structures Life Prediction Program (CARES/Life), and (3) the Abaqus finite element analysis program. MAC/GMC contributes multiscale modeling capabilities and micromechanics relations to determine stresses and deformations at the microscale of the composite material repeating-unit-cell (RUC). CARES/Life contributes statistical multiaxial failure criteria that can be applied to the individual brittle-material constituents of the RUC, and Abaqus is used to model the overall composite structure. For each FEAMAC/CARES simulation trial, the stochastic nature of brittle material strength results in random, discrete damage events that incrementally progress until ultimate structural failure.

[Laminitis with a high degree of osteitis rarefaciens of the distal phalanx in a Fleckvieh bull].

Science.gov (United States)

Kofler, J; Stanek, C

1990-06-01

Chronic laminitis with severe rarefactive osteitis was diagnosed in a nine month old Simmental fattening bull. The condition was observed in all eight weight bearing digits. Clinical, radiological and pathomorphological examinations revealed osteolysis and downward rotation of all the distal phalanges. The condition appears to resemble the several cases of laminitis in Jersey cattle, which have been attributed to hereditary factors. In this case, however, the genetic background was unavailable for evaluation.

Tunable characteristics of bending resonance frequency in magnetoelectric laminated composites

Institute of Scientific and Technical Information of China (English)

Chen Lei; Li Ping; Wen Yu-Mei; Zhu Yong

2013-01-01

As the magnetoelectric (ME) effect in piezoelectric/magnetostrictive laminated composites is mediated by mechanical deformation,the ME effect is significantly enhanced in the vicinity of resonance frequency.The bending resonance frequency (fr) of bilayered Terfenol-D/PZT (MP) laminated composites is studied,and our analysis predicts that (i) the bending resonance frequency of an MP laminated composite can be tuned by an applied dc magnetic bias (Hdc) due to the △E effect; (ii) the bending resonance frequency of the MP laminated composite can be controlled by incorporating FeCuNbSiB layers with different thicknesses.The experimental results show that with Hdc increasing from 0Oe (1 Oe=79.5775 A/m)to 700 Oe,the bending resonance frequency can be shifted in a range of 32.68 kHz ≤ fr ≤ 33.96 kHz.In addition,with the thickness of the FeCuNbSiB layer increasing from 0 μm to 90 μm,the bending resonance frequency of the MP laminated composite gradually increases from 33.66 kHz to 39.18 kHz.This study offers a method of adjusting the strength of dc magnetic bias or the thicknesses of the FeCuNbSiB layer to tune the bending resonance frequency for ME composite,which plays a guiding role in the ME composite design for real applications.

Fatigue crack growth in fiber-metal laminates

Science.gov (United States)

Ma, YuE; Xia, ZhongChun; Xiong, XiaoFeng

2014-01-01

Fiber-metal laminates (FMLs) consist of three layers of aluminum alloy 2024-T3 and two layers of glass/epoxy prepreg, and it (it means FMLs) is laminated by Al alloy and fiber alternatively. Fatigue crack growth rates in notched fiber-metal laminates under constant amplitude fatigue loading were studied experimentally and numerically and were compared with them in monolithic 2024-T3 Al alloy plates. It is shown that the fatigue life of FMLs is about 17 times longer than monolithic 2024-T3 Al alloy plate; and crack growth rates in FMLs panels remain constant mostly even when the crack is long, unlike in the monolithic 2024-T3 Al alloy plates. The formula to calculate bridge stress profiles of FMLs was derived based on the fracture theory. A program by Matlab was developed to calculate the distribution of bridge stress in FMLs, and then fatigue growth lives were obtained. Finite element models of FMLs were built and meshed finely to analyze the stress distributions. Both results were compared with the experimental results. They agree well with each other.

Self-organising aggregates of zebrafish retinal cells for investigating mechanisms of neural lamination.

Science.gov (United States)

Eldred, Megan K; Charlton-Perkins, Mark; Muresan, Leila; Harris, William A

2017-03-15

To investigate the cell-cell interactions necessary for the formation of retinal layers, we cultured dissociated zebrafish retinal progenitors in agarose microwells. Within these wells, the cells re-aggregated within hours, forming tight retinal organoids. Using a Spectrum of Fates zebrafish line, in which all different types of retinal neurons show distinct fluorescent spectra, we found that by 48 h in culture, the retinal organoids acquire a distinct spatial organisation, i.e. they became coarsely but clearly laminated. Retinal pigment epithelium cells were in the centre, photoreceptors and bipolar cells were next most central and amacrine cells and retinal ganglion cells were on the outside. Image analysis allowed us to derive quantitative measures of lamination, which we then used to find that Müller glia, but not RPE cells, are essential for this process. © 2017. Published by The Company of Biologists Ltd.

Resonant responses and chaotic dynamics of composite laminated circular cylindrical shell with membranes

Science.gov (United States)

Zhang, W.; Liu, T.; Xi, A.; Wang, Y. N.

2018-06-01

This paper is focused on the resonant responses and chaotic dynamics of a composite laminated circular cylindrical shell with radially pre-stretched membranes at both ends and clamped along a generatrix. Based on the two-degree-of-freedom non-autonomous nonlinear equations of this system, the method of multiple scales is employed to obtain the four-dimensional nonlinear averaged equation. The resonant case considered here is the primary parametric resonance-1/2 subharmonic resonance and 1:1 internal resonance. Corresponding to several selected parameters, the frequency-response curves are obtained. From the numerical results, we find that the hardening-spring-type behaviors and jump phenomena are exhibited. The jump phenomena also occur in the amplitude curves of the temperature parameter excitation. Moreover, it is found that the temperature parameter excitation, the coupling degree of two order modes and the detuning parameters can effect the nonlinear oscillations of this system. The periodic and chaotic motions of the composite laminated circular cylindrical shell clamped along a generatrix are demonstrated by the bifurcation diagrams, the maximum Lyapunov exponents, the phase portraits, the waveforms, the power spectrums and the Poincaré map. The temperature parameter excitation shows that the Pomeau-Manneville type intermittent chaos occur under the certain initial conditions. It is also found that there exist the twin phenomena between the Pomeau-Manneville type intermittent chaos and the period-doubling bifurcation.

A comparative study on low-velocity impact response of fabric composite laminates

International Nuclear Information System (INIS)

Zhang, Diantang; Sun, Ying; Chen, Li; Pan, Ning

2013-01-01

Highlights: • We examine the low-velocity behavior of single-ply 3D orthogonal woven fabric composites. • Three-coordinate measuring device was used to acquire the 3D topographies. • Single-ply 3D orthogonal woven fabric composites show better impact performance. • Impact properties will increase if in-plane yarns and Z-yarns of single-ply 3D orthogonal woven fabric are optimized. - Abstract: Impact behaviors at low velocity of composite laminates reinforced with fabrics of different architectures are investigated. Unidirectional prepreg, 2D woven and 3D orthogonal fabrics, all formed of Ultrahigh Molecular Weight Polyethylene (UHMWPE) filaments, were selected as reinforcements to form composite laminates using hot pressing technology. Low velocity impact tests were conducted using a drop-weight impact equipment at the energy level of 35 J. A three-coordinate measuring device was employed to determine the volume of plastic deformation and surface dent diameter. The results show that the composite laminates of single-ply 3D orthogonal woven fabric exhibit better energy absorbed capacity and impact damage resistance as compared to those of unidirectional and 2D plain-woven fabric

Enhancement of Gas Barrier Properties of CFRP Laminates Fabricated Using Thin-Ply Prepregs

Science.gov (United States)

横関, 智弘; 高木, 智宏; 吉村, 彰記; Ogasawara, Toshio; 荻原, 慎二

Composite laminates manufactured using thin-ply prepregs are expected to have superior resistance properties against microcracking compared to those using standard prepregs. In this study, comparative investigations are presented on the microcrack accumulation and gas leakage characteristics of CFRP laminates fabricated using standard and thin-ply prepregs, consisting of high-performance carbon fiber and toughened epoxy, as a fundamental research on the cryogenic composite tanks for future space vehicles. It was shown that laminates using thin-ply prepregs exhibited much higher strain at microcrack initiation compared to those using standard prepregs at room and cryogenic temperatures. In addition, helium gas leak tests using CFRP laminated tubular specimens subjected to quasi-static tension loadings were performed. It was demonstrated that CFRP laminates using thin-ply prepregs have higher gas barrier properties than those using standard prepregs.

Tungsten foil laminate for structural divertor applications – Joining of tungsten foils

Energy Technology Data Exchange (ETDEWEB)

Reiser, Jens, E-mail: jens.reiser@kit.edu [Karlsruhe Institute of Technology (KIT), Institute for Applied Materials (IAM-AWP) (Germany); Rieth, Michael; Möslang, Anton; Dafferner, Bernhard; Hoffmann, Jan [Karlsruhe Institute of Technology (KIT), Institute for Applied Materials (IAM-AWP) (Germany); Mrotzek, Tobias; Hoffmann, Andreas [PLANSEE SE, Reutte (Austria); Armstrong, D.E.J.; Yi, Xiaoou [University of Oxford, Department of Materials (United Kingdom)

2013-05-15

This paper is the fourth in our series on tungsten laminates. The aim of this paper is to discuss laminate synthesis, meaning the joining of tungsten foils. It is obvious that the properties of the tungsten laminate strongly depend on the combination of (i) interlayer and (ii) joining technology, as this combination defines (i) the condition of the tungsten foil after joining (as-received or recrystallised) as well as (ii) the characteristics of the interface between the tungsten foil and the interlayer (wettability or diffusion leading to a solid solution or the formation of intermetallics). From the example of tungsten laminates joined by brazing with (i) an eutectic silver copper brazing filler, (ii) copper, (iii) titanium, and (iv) zirconium, the microstructure will be discussed, with special focus on the interface. Based on our assumptions of the mechanism of the extraordinary ductility of tungsten foil we present three syntheses strategies and make recommendations for the synthesis of high temperature tungsten laminates.

Vibrations of laminated composite thick shells of revolution having meridionally varying curvature

International Nuclear Information System (INIS)

Suzuki, Katsuyoshi; Shikanai, Genji; Baba, Iwato

1998-01-01

An exact solution is presented for solving free vibrations of laminated composite thick shells of revolution having meridionally varying curvature. Based on the thick lamination theory considering the shear deformation and rotary inertia, equations of motion and boundary conditions are obtained from the stationary conditions of the Lagrangian. The equations of motion are solved exactly by using a power series expansion for symmetrically laminated cross-ply shells. Frequencies and mode shapes of shells of revolution having elliptical and parabolical meridians are presented for both ends clamped, and the effects of shear deformation and rotary inertia are discussed by comparing the results from the present theory with those from the thin lamination theory. (author)

Optimum design of laminated composite under axial compressive load

Indian Academy of Sciences (India)

In the present study optimal design of composite laminates, with and without rectangular cut-out, is carried out for maximizing the buckling load. Optimization study is carried out for obtaining the maximum buckling load with design variables as ply thickness, cut-out size and orientation of cut-out with respect to laminate.

A case of Lamin C gene-mutation with preserved systolic function and ventricular dysrrhythmia

Directory of Open Access Journals (Sweden)

Kevin Kit Ng

2013-02-01

Full Text Available Lamin A/C gene-related cardiomyopathy is associated with progressive heart failure and malignant arrhythmias. Current guidelines advise the use of implantable defibrillators to prevent arrhythmogenic sudden cardiac death only in situations where there is evidence of severe left ventricular dysfunction. We describe a case of a woman with genetically confirmed Lamin C deficiency with preserved left ventricular function in whom an implantable defibrillator was inserted and within a month of implantation was used to terminate symptomatic ventricular tachycardia.

The role of nonlinear viscoelasticity on the functionality of laminating shortenings

Energy Technology Data Exchange (ETDEWEB)

Macias-Rodriguez, Braulio A.; Peyronel, Fernanda; Marangoni, Alejandro G.

2017-11-01

The rheology of fats is essential for the development of homogeneous and continuous layered structures of doughs. Here, we define laminating shortenings in terms of rheological behavior displayed during linear-to-nonlinear shear deformations, investigated by large amplitude oscillatory shear rheology. Likewise, we associate the rheological behavior of the shortenings with structural length scales elucidated by ultra-small angle x-ray scattering and cryo-electron microscopy. Shortenings exhibited solid-like viscoelastic and viscoelastoplastic behaviors in the linear and nonlinear regimes respectively. In the nonlinear region, laminating shortenings dissipated more viscous energy (larger normalized dynamic viscosities) than a cake bakery shortening. The fat solid-like network of laminating shortening displayed a three-hierarchy structure and layered crystal aggregates, in comparison to two-hierarchy structure and spherical-like crystal aggregates of a cake shortening. We argue that the observed rheology, correlated to the structural network, is crucial for optimal laminating performance of shortenings.

A low frequency vibration energy harvester using magnetoelectric laminate composite

International Nuclear Information System (INIS)

Ju, Suna; Chae, Song Hee; Choi, Yunhee; Lee, Seungjun; Ji, Chang-Hyeon; Lee, Hyang Woon

2013-01-01

In this paper, we present a vibration energy harvester using magnetoelectric laminate composite and a springless spherical permanent magnet as a proof mass. The harvester utilizes a freely movable spherical permanent magnet to transform external vibration into a time varying magnetic field applied to the magnetoelectric transducer. The laminate composite consists of a Ni–Mn–Ga-based MSMA (magnetic shape memory alloy) element and a PZT (lead zirconate titanate) plate. A proof-of-concept harvester has been fabricated and characterized at various input accelerations and frequencies. A maximum open circuit voltage of 1.18 V has been obtained in response to a 3g vibration at 17 Hz with the fabricated device. Moreover, a maximum output voltage of 10.24 V and output power of 4.1 μW have been achieved on a 950 Ω load, when the fabricated energy harvester was mounted on a smartphone and shaken by hand. (paper)

Probabilistic Modelling of Fatigue Life of Composite Laminates Using Bayesian Inference

DEFF Research Database (Denmark)

Dimitrov, Nikolay Krasimirov; Kiureghian, Armen Der

2014-01-01

A probabilistic model for estimating the fatigue life of laminated composite plates subjected to constant-amplitude or variable-amplitude loading is developed. The model is based on lamina-level input data, making it possible to predict fatigue properties for a wide range of laminate configuratio...

On Poisson's ratio for metal matrix composite laminates. [aluminum boron composites

Science.gov (United States)

Herakovich, C. T.; Shuart, M. J.

1978-01-01

The definition of Poisson's ratio for nonlinear behavior of metal matrix composite laminates is discussed and experimental results for tensile and compressive loading of five different boron-aluminum laminates are presented. It is shown that there may be considerable difference in the value of Poisson's ratio as defined by a total strain or an incremental strain definition. It is argued that the incremental definition is more appropriate for nonlinear material behavior. Results from a (0) laminate indicate that the incremental definition provides a precursor to failure which is not evident if the total strain definition is used.

Field performance of timber bridges. 9, Big Erick`s stress-laminated deck bridge

Science.gov (United States)

J. A. Kainz; J. P. Wacker; M. Nelson

The Big Erickas bridge was constructed during September 1992 in Baraga County, Michigan. The bridge is 72 ft long, 16 ft wide, and consists of three simple spans: two stress-laminated deck approach spans and a stress-laminated box center span. The bridge is unique in that it is one of the first known stress-laminated timber bridge applications to use Eastern Hemlock...

Laminated piezoelectric transformer

Science.gov (United States)

Vazquez Carazo, Alfredo (Inventor)

2006-01-01

A laminated piezoelectric transformer is provided using the longitudinal vibration modes for step-up voltage conversion applications. The input portions are polarized to deform in a longitudinal plane and are bonded to an output portion. The deformation of the input portions is mechanically coupled to the output portion, which deforms in the same longitudinal direction relative to the input portion. The output portion is polarized in the thickness direction relative its electrodes, and piezoelectrically generates a stepped-up output voltage.

Detection of layup errors in prepreg laminates using shear ultrasonic waves

Science.gov (United States)

Hsu, David K.; Fischer, Brent A.

1996-11-01

The highly anisotropic elastic properties of the plies in a composite laminate manufactured from unidirectional prepregs interact strongly with the polarization direction of shear ultrasonic waves propagating through its thickness. The received signals in a 'crossed polarizer' transmission configuration are particularly sensitive to ply orientation and layup sequence in a laminate. Such measurements can therefore serve as an NDE tool for detecting layup errors. For example, it was shown experimentally recently that the sensitivity for detecting the presence of misoriented plies is better than one ply out of a 48-ply laminate of graphite epoxy. A physical model based on the decomposition and recombination of the shear polarization vector has been constructed and used in the interpretation and prediction of test results. Since errors should be detected early in the manufacturing process, this work also addresses the inspection of 'green' composite laminates using electromagnetic acoustic transducers (EMAT). Preliminary results for ply error detection obtained with EMAT probes are described.

Bonding techniques for flexural strengthening of R.C. beams using CFRP laminates

Directory of Open Access Journals (Sweden)

Alaa Morsy

2013-09-01

Full Text Available This paper presents an experimental study of an alternative method of attaching FRP laminates to reinforced concrete beams by the way of fasting steel rivets through the FRP laminate and concrete substrate. Five full scale R.C. beams were casted and strengthened in flexural using FRP laminate bonded with conventional epoxy and compared with other beams strengthened with FRP laminate and bonded with fastener “steel rivets” of 50 mm length and 10 mm diameter. Based on experimental evidence the beam strengthened with conventional bonding methods failed due to de-bonding with about 13% increase over the un-strengthened beam. On the other hand, the beams strengthened with FRP laminate and bonded by four steel fastener rivets only failed by de-bonding also but at higher flexural capacity with increase 19% over the un-strengthened beam.

The D4Z4 macrosatellite repeat acts as a CTCF and A-type lamins-dependent insulator in facio-scapulo-humeral dystrophy.

Directory of Open Access Journals (Sweden)

Alexandre Ottaviani

2009-02-01

Full Text Available Both genetic and epigenetic alterations contribute to Facio-Scapulo-Humeral Dystrophy (FSHD, which is linked to the shortening of the array of D4Z4 repeats at the 4q35 locus. The consequence of this rearrangement remains enigmatic, but deletion of this 3.3-kb macrosatellite element might affect the expression of the FSHD-associated gene(s through position effect mechanisms. We investigated this hypothesis by creating a large collection of constructs carrying 1 to >11 D4Z4 repeats integrated into the human genome, either at random sites or proximal to a telomere, mimicking thereby the organization of the 4q35 locus. We show that D4Z4 acts as an insulator that interferes with enhancer-promoter communication and protects transgenes from position effect. This last property depends on both CTCF and A-type Lamins. We further demonstrate that both anti-silencing activity of D4Z4 and CTCF binding are lost upon multimerization of the repeat in cells from FSHD patients compared to control myoblasts from healthy individuals, suggesting that FSHD corresponds to a gain-of-function of CTCF at the residual D4Z4 repeats. We propose that contraction of the D4Z4 array contributes to FSHD physio-pathology by acting as a CTCF-dependent insulator in patients.

78 FR 23591 - Certain Prepregs, Laminates, and Finished Circuit Boards

Science.gov (United States)

2013-04-19

... INTERNATIONAL TRADE COMMISSION [Investigation No. 337-TA-659 (Enforcement)] Certain Prepregs... United States after importation of certain prepregs, laminates, and finished circuit boards that infringe... prepregs and laminates that are the subject of the investigation or that otherwise infringe, induce, and/or...

Repetitive disruptions of the nuclear envelope invoke temporary loss of cellular compartmentalization in laminopathies

NARCIS (Netherlands)

De Vos, W.H.; Houben, F.; Kamps, M.; Malhas, A.; Verheyen, F.; Cox, J.; Manders, E.M.M.; Verstraeten, V.L.R.M.; van Steensel, M.A.M.; Marcelis, C.L.M.; van den Wijngaard, A.; Vaux, D.J.; Ramaekers, F.C.S.; Broers, J.L.V.

2011-01-01

The nuclear lamina provides structural support to the nucleus and has a central role in nuclear organization and gene regulation. Defects in its constituents, the lamins, lead to a class of genetic diseases collectively referred to as laminopathies. Using live cell imaging, we observed the

Laminated lumber may be more profitable than sawn lumber

Science.gov (United States)

P. Koch

1976-01-01

By laminating 1/4-in. rotary-cut veneer into structural lumber, manufacturers can expand lumber output by at least 30% without increasing volume logged. The idea merits intensive study. Manufacturing plus raw material costs should total about $142/Mbf; sales price for desirable widths and lengths of the strong laminated product should approach or exceed $200/Mbf.

Process monitoring of glass reinforced polypropylene laminates using fiber Bragg gratings

KAUST Repository

Mulle, Matthieu; Wafai, Husam; Yudhanto, Arief; Lubineau, Gilles; Yaldiz, R.; Schijve, W.; Verghese, N.

2015-01-01

Hot-press molding of glass-fiber-reinforced polypropylene (GFPP) laminates was monitored using longitudinally and transversely embedded fiber Bragg gratings (FBGs) at different locations in unidirectional laminates. The optical sensors proved

Preparation of Chitin-PLA laminated composite for implantable application

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Romana Nasrin

2017-12-01

Full Text Available The present study explores the possibilities of using locally available inexpensive waste prawn shell derived chitin reinforced and bioabsorbable polylactic acid (PLA laminated composites to develop new materials with excellent mechanical and thermal properties for implantable application such as in bone or dental implant. Chitin at different concentration (1–20% of PLA reinforced PLA films (CTP were fabricated by solvent casting process and laminated chitin-PLA composites (LCTP were prepared by laminating PLA film (obtained by hot press method with CTP also by hot press method at 160 °C. The effect of variation of chitin concentration on the resulting laminated composite's behavior was investigated. The detailed physico-mechanical, surface morphology and thermal were assessed with different characterization technique such as FT-IR, XRD, SEM and TGA. The FTIR spectra showed the characteristic peaks for chitin and PLA in the composites. SEM images showed an excellent dispersion of chitin in the films and composites. Thermogravimetric analysis (TGA showed that the complete degradation of chitin, PLA film, 5% chitin reinforced PLA film (CTP2 and LCTP are 98%, 95%, 87% and 98% respectively at temperature of 500 °C. The tensile strength of the LCTP was found 25.09 MPa which is significantly higher than pure PLA film (18.55 MPa and CTP2 film (8.83 MPa. After lamination of pure PLA and CTP2 film, the composite (LCTP yielded 0.265–1.061% water absorption from 30 min to 24 h immerse in water that is much lower than PLA and CTP. The increased mechanical properties of the laminated films with the increase of chitin content indicated good dispersion of chitin into PLA and strong interfacial actions between the polymer and chitin. The improvement of mechanical properties and the results of antimicrobial and cytotoxicity of the composites also evaluated and revealed the composite would be a suitable candidate for implant application in biomedical

Preparation of Chitin-PLA laminated composite for implantable application.

Science.gov (United States)

Nasrin, Romana; Biswas, Shanta; Rashid, Taslim Ur; Afrin, Sanjida; Jahan, Rumana Akhter; Haque, Papia; Rahman, Mohammed Mizanur

2017-12-01

The present study explores the possibilities of using locally available inexpensive waste prawn shell derived chitin reinforced and bioabsorbable polylactic acid (PLA) laminated composites to develop new materials with excellent mechanical and thermal properties for implantable application such as in bone or dental implant. Chitin at different concentration (1-20% of PLA) reinforced PLA films (CTP) were fabricated by solvent casting process and laminated chitin-PLA composites (LCTP) were prepared by laminating PLA film (obtained by hot press method) with CTP also by hot press method at 160 °C. The effect of variation of chitin concentration on the resulting laminated composite's behavior was investigated. The detailed physico-mechanical, surface morphology and thermal were assessed with different characterization technique such as FT-IR, XRD, SEM and TGA. The FTIR spectra showed the characteristic peaks for chitin and PLA in the composites. SEM images showed an excellent dispersion of chitin in the films and composites. Thermogravimetric analysis (TGA) showed that the complete degradation of chitin, PLA film, 5% chitin reinforced PLA film (CTP2) and LCTP are 98%, 95%, 87% and 98% respectively at temperature of 500 °C. The tensile strength of the LCTP was found 25.09 MPa which is significantly higher than pure PLA film (18.55 MPa) and CTP2 film (8.83 MPa). After lamination of pure PLA and CTP2 film, the composite (LCTP) yielded 0.265-1.061% water absorption from 30 min to 24 h immerse in water that is much lower than PLA and CTP. The increased mechanical properties of the laminated films with the increase of chitin content indicated good dispersion of chitin into PLA and strong interfacial actions between the polymer and chitin. The improvement of mechanical properties and the results of antimicrobial and cytotoxicity of the composites also evaluated and revealed the composite would be a suitable candidate for implant application in biomedical sector.

Testing the fidelity of laminations as a proxy for oxygen concentration in the Bering Sea over millennial to orbital timescales

Science.gov (United States)

Black, A. E.; Baranow, N.; Amdur, S.; Cook, M. S.

2017-12-01

Ocean circulation and biological productivity play an important role in the climate system through their contribution to global heat transport and air-sea exchange of CO­2. Oceanic oxygen concentration provides insight to ocean circulation and biological productivity. Sediment laminations provide a valuable proxy for local oceanic oxygen concentration. Many sediment cores from the Pacific Ocean are laminated from the last deglaciation, but previous studies have not provided an in-depth examination of laminations over many glacial and interglacial (G/IG) cycles. Typically, studies to date that consider bioturbation as a proxy for oxygen concentration have only considered one sediment core from a site, leaving ambiguity as to whether laminations faithfully record local oxygen levels. With sediment cores from three different holes (A, C, D) on the northern Bering Slope from IODP site U1345 (1008m), we investigate how faithfully laminations record oxygen concentration. We assign a bioturbation index from 1 to 4 for 1-cm intervals for the cores from each of the three holes and align the holes based on physical properties data. We find that the bioturbation is relatively consistent (within one bioturbation unit) between holes, suggesting that laminations may be a faithful, if not perfect, proxy for local oxygen concentration. After examining laminations from a complete hole, representing over 500,000 years, there seems to be no consistent pattern of laminations during the past five glacial cycles, suggesting there is no consistent pattern to oxygen concentration during glacial periods in the northern Bering Slope. Thus, hypotheses on ocean circulation and productivity in the northern Bering Sea from the last deglaciation may not apply to previous G/IG cycles.

Experimental verification of a progressive damage model for composite laminates based on continuum damage mechanics. M.S. Thesis Final Report

Science.gov (United States)

Coats, Timothy William

1994-01-01

Progressive failure is a crucial concern when using laminated composites in structural design. Therefore the ability to model damage and predict the life of laminated composites is vital. The purpose of this research was to experimentally verify the application of the continuum damage model, a progressive failure theory utilizing continuum damage mechanics, to a toughened material system. Damage due to tension-tension fatigue was documented for the IM7/5260 composite laminates. Crack density and delamination surface area were used to calculate matrix cracking and delamination internal state variables, respectively, to predict stiffness loss. A damage dependent finite element code qualitatively predicted trends in transverse matrix cracking, axial splits and local stress-strain distributions for notched quasi-isotropic laminates. The predictions were similar to the experimental data and it was concluded that the continuum damage model provided a good prediction of stiffness loss while qualitatively predicting damage growth in notched laminates.

Static and dynamic through thickness lamina properties of thick laminates

NARCIS (Netherlands)

Lahuerta, F.; Nijssen, R.P.L.; Van der Meer, F.P.; Sluys, L.J.

2015-01-01

Thick laminates are increasingly present in large composites structures such as wind turbine blades. Different factors are suspected to be involved in the decreased static and dynamic performance of thick laminates. These include the effect of self-heating, the scaling effect, and the manufacturing

Fatigue damage mechanism and strength of woven laminates

International Nuclear Information System (INIS)

Xiao, J.; Bathias, C.

1993-01-01

The apparent secant stiffness changes with the cyclic number for both unnotched and notched woven laminated specimens (two orthotropic and one quasi-isotropic) during tensile fatigue test at a fixed ratio of maximum fatigue load to UTS were observed. The observable damage initiation and evolution as a function of the cyclic number were directly measured at the notched specimen surface with a video-camera system. The fatigue strengths of the unnotched and notched specimens were determined. The results show that the normalized apparent secant stiffness change curves as a function of cyclic numbers can be divided into three stages. For the first and the second stages in notched specimens and for total life of unnotched specimens, the damage has not been evidently observed and certainly verified with the traditional experimental methods such as radiography and microscopy although many acoustic emission signals can be obtained. The last stage for the notched specimens (N/Nf>0.4, the secant stiffness decreases fast) corresponds to the initiation and evolution of the observable damages. The fatigue strength of these woven composite laminates is dominated by the third stage during which the observable damage develops along the specimen ligament until fracture. During the third stage, a critical dimension at the specimen ligament and a life threshold can be found beyond which a final catastrophic fracture will immediately occur. The quasi-isotropic laminate is of a fatigue strength lower than the two orthotropic laminates of which the fatigue strengths are approaching to each other. The fatigue life is also influenced by the stacking sequences. (orig.)

Environmental Effects on Flutter Characteristics of Laminated Composite Rectangular and Skew Panels

Directory of Open Access Journals (Sweden)

T.V.R. Chowdary

1996-01-01

Full Text Available A finite element method is presented for predicting the flutter response of laminated composite panels subjected to moisture concentration and temperature. The analysis accounts for material properties at elevated temperature and moisture concentration. The analysis is based on the first-order approximation to the linear piston theory and laminated plate theory that includes shear deformation. Both rectangular and skew panels are considered. Stability boundaries at moisture concentrations and temperatures for various lamination schemes and boundary conditions are discussed.

Hygrothermal Effect on Mechanical and Fatigue Properties of laminated Lower Limb Socket and Bacteria Growth

Directory of Open Access Journals (Sweden)

Fadhel Abbas Abdullah

2016-12-01

Full Text Available In this work, hygrothermal effect on the mechanical and fatigue properties of prosthetic socket lamination and its effect on the bacteria growth were studied. Two laminations composite materials were used in manufacturing prosthetic socket by using vacuum device. The reinforced materials of these laminations were perlon and carbon nanopowder (CNP while the matrix material was polyurethane resin. Tests performed in this work were the moisture absorption properties test to calculate percent moisture content according to ASTM 5229, tensile and fatigue tests with and without the hygrothermal effect to find the mechanical and fatigue properties, and the bacteria growth test under the hygrothermal effect to calculate the number of bacteria on the laminations. The results showed that the lamination (10 perlon+1 wt % CNP has mechanical properties than lamination (10 perlon with and without hygrothermal effect. The mechanical and fatigue properties for the two laminations were decreasing with increasing temperature and moisture.. Adding carbon nanopowder to the lamination (10 perlon increased ultimate stress, modulus of elastic, and endurance limit by (1.36, 2.35, and2.72 time respectively. Finally, the results showed that the Staphylococcus aureus growth increases with increasing temperature and moisture on the two laminations used in manufacturing prosthetic socket, and adding carbon nanopowder also increased the Staphylococcus aureus growth on the lamination.

Fracture Strength of Indirect Resin Composite Laminates to Teeth with Existing Restorations : An Evaluation of Conditioning Protocols

NARCIS (Netherlands)

Mese, Ayse; Ozcan, Mutlu

2009-01-01

Purpose: This study evaluated the fracture strength and failure types of indirect resin-based composite laminates bonded to teeth with aged Class III composite restorations that were conditioned according to various protocols. Materials and Methods: Maxillary central incisors (N = 60) with

Self-heating forecasting for thick laminates testing coupons in fatigue

NARCIS (Netherlands)

Lahuerta, F.; Westphal, T.; Nijssen, R.P.L.

2012-01-01

Thick laminate sections can be found from the tip to the root in most common wind turbine blade designs. Obtaining accurate and reliable design data for thick laminates is subject of investigations. Due to the poor thermal conductivity properties of composites and the material self-heating that

Repair and Strengthening by Use of Superficial Fixed Laminates of Cracked Masonry Walls Sheared Horizontally-Laboratory Tests

International Nuclear Information System (INIS)

Kubica, Jan; Kwiecien, Arkadiusz; Zajac, Boguslaw

2008-01-01

There are many methods of crack repairing in masonry structures. One of them is repair and strengthening by using of superficial fixed laminates, especially in case of masonry walls with plastering on their both sides. The initial laboratory tests of three different types of strengthening of diagonal cracked masonry wallettes are presented. Tests concerned three clay brick masonry walls subjected to horizontal shearing with two levels of precompression and strengthened by flexible polymer injection, superficial glass fixed by polymer fibre laminate plates and using of CRFP strips stiff fixed to the wall surface by polymer and stiff resin epoxy fixing are presented and discussed

Evaluation of Interlaminar Stresses in Composite Laminates with a Bolt-Filled Hole Using a Linear Elastic Traction-Separation Description

Directory of Open Access Journals (Sweden)

Yong Cao

2017-01-01

Full Text Available Determination of the local interlaminar stress distribution in a laminate with a bolt-filled hole is helpful for optimal bolted joint design, due to the three-dimensional (3D nature of the stress field near the bolt hole. A new interlaminar stress distribution phenomenon induced by the bolt-head and clamp-up load, which occurs in a filled-hole composite laminate, is investigated. In order to efficiently evaluate interlaminar stresses under the complex boundary condition, a calculation strategy that using zero-thickness cohesive interface element is presented and validated. The interface element is based on a linear elastic traction-separation description. It is found that the interlaminar stress concentrations occur at the hole edge, as well as the interior of the laminate near the periphery of the bolt head. In addition, the interlaminar stresses near the periphery of the bolt head increased with an increase in the clamp-up load, and the interlaminar normal and shear stresses are not at the same circular position. Therefore, the clamp-up load cannot improve the interlaminar stress distribution in the laminate near the periphery of the bolt head, although it can reduce the magnitude of the interlaminar shear stress at the hole edge. Thus, the interlaminar stress distribution phenomena may lead to delamination initiation in the laminate near the periphery of the bolt head, and should be considered in composite bolted joint design.

Aged induced pluripotent stem cell (iPSCs) as a new cellular model for studying premature aging.

Science.gov (United States)

Petrini, Stefania; Borghi, Rossella; D'Oria, Valentina; Restaldi, Fabrizia; Moreno, Sandra; Novelli, Antonio; Bertini, Enrico; Compagnucci, Claudia

2017-05-31

Nuclear integrity and mechanical stability of the nuclear envelope (NE) are conferred by the nuclear lamina, a meshwork of intermediate filaments composed of A- and B-type lamins, supporting the inner nuclear membrane and playing a pivotal role in chromatin organization and epigenetic regulation. During cell senescence, nuclear alterations also involving NE architecture are widely described. In the present study, we utilized induced pluripotent stem cells (iPSCs) upon prolonged in vitro culture as a model to study aging and investigated the organization and expression pattern of NE major constituents. Confocal and four-dimensional imaging combined with molecular analyses, showed that aged iPSCs are characterized by nuclear dysmorphisms, nucleoskeletal components (lamin A/C-prelamin isoforms, lamin B1, emerin, and nesprin-2) imbalance, leading to impaired nucleo-cytoplasmic MKL1 shuttling, actin polymerization defects, mitochondrial dysfunctions, SIRT7 downregulation and NF-kBp65 hyperactivation. The observed age-related NE features of iPSCs closely resemble those reported for premature aging syndromes (e.g., Hutchinson-Gilford progeria syndrome) and for somatic cell senescence. These findings validate the use of aged iPSCs as a suitable cellular model to study senescence and for investigating therapeutic strategies aimed to treat premature aging.

Control of free-edge interlaminar stresses in composite laminates using piezoelectric actuators

International Nuclear Information System (INIS)

Huang, Bin; Soo Kim, Heung

2014-01-01

The control of free-edge interlaminar stresses in laminated composite structures using a stress function-based approach is proposed. The assumed stress fields satisfy pointwise traction and free boundary conditions at surfaces. Governing equations are derived using the principle of complementary virtual work. A general eigenvalue solution procedure was adopted to obtain accurate stress states of the laminated composite structure. The results obtained from the proposed method were compared with those obtained by three-dimensional finite element analyses. It was found that interlaminar stresses generated by mechanical loadings could be significantly reduced by applying proper electric fields to piezoelectric actuators, which were surface bonded or embedded in composite laminates. Locations of piezoelectric actuators also influenced the distributions of interlaminar stresses. The results provided that piezoelectric actuators have potential in the application to actively control interlaminar stresses in composite laminates. (paper)

Histopathology of dairy cows' hooves with signs of naturally acquired laminitis

Directory of Open Access Journals (Sweden)

Heloisa M.F. Mendes

2013-05-01

Full Text Available The purpose of this study was to investigate histological changes in dairy cows' hooves with or without injuries from naturally acquired laminitis. Cull cows with no clinical signs of hoof abnormalities (G1, n=9 and those with macroscopic lesions associated with laminitis without (G2, n=23 or with lameness (G3, n=7 were used in the study. After slaughter, samples of dermo-epidermal junctions of sole, axial and dorsal regions of the hoof were obtained and histologically processed using HE and PAS staining. Congestion, hemorrhage and inflammatory infiltrate in the dermis of sole, axial and dorsal regions were blindly and semiquantitatively evaluated by the same researcher. Inflammatory infiltrate was evaluated in the dermal laminae of axial and dorsal regions. The morphology of epidermal cells and the presence of irregularities in three regions of the basement membrane (BM length were examined using PAS staining. Scores of lesions in different regions of the hoof in the same group and in different groups for each region of the hoof were compared using non-parametric analyses. Inflammatory infiltrate in the dermis of all regions of the hoof was detected in all groups with no significant statistical difference. Cows with no clinical signs of hoof abnormalities secondary to laminitis (G1 have inflammation scores and epidermal cell changes similar to those of groups with laminitis injuries, suggesting the existence of a prodromal phase for this disease in bovines. BM had irregularities with a variable intensity along its length, however, with no difference among groups. The pattern of BM irregularities found has not been reported so far and does not resemble the BM collapse described in horses and cattle with induced acute laminitis. Is it concluded that even in the absence of macroscopic hoof signs associated to laminitis, dairy cows have histological injuries compatible with inflammation of the dermo-epidermal junction as in affected animals

Acute bovine laminitis: a new induction model using alimentary oligofructose overload.

Science.gov (United States)

Thoefner, M B; Pollitt, C C; Van Eps, A W; Milinovich, G J; Trott, D J; Wattle, O; Andersen, P H

2004-09-01

Twelve dairy heifers were used to examine the clinical response of an alimentary oligofructose overload. Six animals were divided into 3 subgroups, and each was given a bolus dose of 13, 17, or 21 g/kg of oligofructose orally. The control group (n = 6) was sham-treated with tap water. Signs of lameness, cardiovascular function, and gastrointestinal function were monitored every 6 h during development of rumen acidosis. The heifers were euthanized 48 and 72 h after administration of oligofructose. All animals given oligofructose developed depression, anorexia, and diarrhea 9 to 39 h after receiving oligofructose. By 33 to 45 h after treatment, the feces returned to normal consistency and the heifers began eating again. Animals given oligofructose developed transient fever, severe metabolic acidosis, and moderate dehydration, which were alleviated by supportive therapy. Four of 6 animals given oligofructose displayed clinical signs of laminitis starting 39 to 45 h after receiving oligofructose and lasting until euthanasia. The lameness was obvious, but could easily be overlooked by the untrained eye, because the heifers continued to stand and walk, and did not interrupt their eating behavior. No positive pain reactions or lameness were seen in control animals. Based on these results, we conclude that an alimentary oligofructose overload is able to induce signs of acute laminitis in cattle. This model offers a new method, which can be used in further investigation of the pathogenesis and pathophysiology of bovine laminitis.

Safety of WWER type nuclear power plants - viewing from Hungary

International Nuclear Information System (INIS)

Voeroess, L.

1991-01-01

An evaluation of WWER type nuclear power plants operating in Hungary is given, relative to the safety requirements accepted internationally; how safe can they be regarded and what can be done to assure a high level of safety in all case. After an overview of general safety criteria, an overall description of WWER-440 type nuclear reactors is presented. Design safety, operational safety issues are treated in detail. Safety inspection and safety-related research and development is discussed. Regarding the future, five different issues associated with nuclear reactor safety should be considered. (R.P.) 20 refs.; 12 figs.; 3 tabs

Estimation of physical properties of laminated composites via the method of inverse vibration problem

Energy Technology Data Exchange (ETDEWEB)

Balci, Murat [Dept. of Mechanical Engineering, Bayburt University, Bayburt (Turkmenistan); Gundogdu, Omer [Dept. of Mechanical Engineering, Ataturk University, Erzurum (Turkmenistan)

2017-01-15

In this study, estimation of some physical properties of a laminated composite plate was conducted via the inverse vibration problem. Laminated composite plate was modelled and simulated to obtain vibration responses for different length-to-thickness ratio in ANSYS. Furthermore, a numerical finite element model was developed for the laminated composite utilizing the Kirchhoff plate theory and programmed in MATLAB for simulations. Optimizing the difference between these two vibration responses, inverse vibration problem was solved to obtain some of the physical properties of the laminated composite using genetic algorithms. The estimated parameters are compared with the theoretical results, and a very good correspondence was observed.

Estimation of physical properties of laminated composites via the method of inverse vibration problem

International Nuclear Information System (INIS)

Balci, Murat; Gundogdu, Omer

2017-01-01

In this study, estimation of some physical properties of a laminated composite plate was conducted via the inverse vibration problem. Laminated composite plate was modelled and simulated to obtain vibration responses for different length-to-thickness ratio in ANSYS. Furthermore, a numerical finite element model was developed for the laminated composite utilizing the Kirchhoff plate theory and programmed in MATLAB for simulations. Optimizing the difference between these two vibration responses, inverse vibration problem was solved to obtain some of the physical properties of the laminated composite using genetic algorithms. The estimated parameters are compared with the theoretical results, and a very good correspondence was observed

Performance of stress-laminated timber highway bridges in cold climates

Science.gov (United States)

James P. Wacker

2009-01-01

This paper summarizes recent laboratory and field data studies on thermal performance of stress-laminated timber highway bridges. Concerns about the reliability of stress-laminated deck bridges when exposed to sub-freezing temperatures triggered several investigations. Two laboratory studies were conducted to study the effects of wood species, preservative, moisture...

The economic potential of a cassette-type-reactor-installed nuclear ice-breaking container ship

International Nuclear Information System (INIS)

Kondo, Koichi; Takamasa, Tomoji

1999-01-01

An improved cassette-type marine reactor MRX (Marine Reactor X) which is currently researched and developed by the Japan Atomic Energy Research Institute is designed to be easily removed and transferred to another ship. If the reactor in a nuclear-powered ship, which is the reason for its higher cost, were replaced by the cassette-type-MRX, the reusability of the MRX would reduce the cost difference between nuclear-powered and diesel ships. As an investigation of one aspect of a cassette-type MRX, we attempted in this study to do an economic review of an MRX-installed nuclear-powered ice-breaking container ship sailing via the Arctic Ocean. The transportation cost between the Far East and Europe to carry one TEU (twenty-foot-equivalent container unit) over the entire life of the ship for an MRX (which is used for a 20-year period)-installed container ship sailing via the Arctic Ocean is about 70% higher than the Suez Canal diesel ship, carrying 8,000 TEU and sailing at 25 knots, and about 10% higher than the Suez Canal diesel ship carrying 4,000 TEU and sailing at 34 knots. The cost for a cassette-type-MRX (which is used for a 40-year period, removed and transferred to a second ship after being used for 20 years in the first ship)-installed nuclear-powered container ship is about 7% lower than that for the one operated for 20 years. Considering any loss or reduction in sales opportunities through the extension of the transportation period, the nuclear-powered container ship via the Arctic Sea is a more suitable means of transportation than a diesel ship sailing at 25 knots via the Suez Canal when the value of the commodities carried exceeds 2,800 dollars per freight ton. (author)

Fabrication of CFRP/Al Active Laminates

Science.gov (United States)

Asanuma, Hiroshi; Haga, Osamu; Ohira, Junichiro; Takemoto, Kyosuke; Imori, Masataka

This paper describes fabrication and evaluation of the active laminate. It was made by hot-pressing of an aluminum plate as a high CTE material, a unidirectional CFRP prepreg as a low CTE material and an electric resistance heater, a KFRP prepreg as a low CTE material and an insulator between them, and copper foils as electrodes. In this study, fabricating conditions and performances such as curvature change and output force were examined. Under optimized fabricating conditions, it became clear that 1) the curvature of the active laminate linearly changes as a function of temperature, between room temperature and its hot pressing temperature without hysteresis by electric resistance heating of carbon fiber in the CFRP layer and cooling, and 2) the output force against a fixed punch almost linearly increases with increasing temperature during heating from 313K up to around the glass transition temperature of the epoxy matrix.

Subclinical Laminitis in Dairy Cattle: 205 Selected Cases

OpenAIRE

BAKIR, Ali BELGE Bahtiyar

2005-01-01

The economic importance of lameness in dairy cattle has newly been recognized in Turkey. Lameness incidence in Turkey has been reported to be between 13% and 58%, which is similar to that of other countries where 4% and 55% incidence rates have been reported. The objective of this study was to determine the prevalence of sole lesions associated with subclinical laminitis in the hooves of dairy cattle in Van, Turkey. The risk factors for subclinical laminitis are proposed and discussed. The so...

Failure modes of laminate structures

Energy Technology Data Exchange (ETDEWEB)

Gordon, L.B.; Druce, R.L.; Wilson, M.J.

1987-06-01

Laminate structures composed of alternating thin layers of conductor and dielectric material are commonly used in energy storage and transmission components. The failure of the dielectric layers in regions of high field stress, with applied 60 Hz ac, dc and impulse voltages, was studied. Several geometries were compared, including staggered and flush edges. Electrical trees developed between the laminated dielectric layers. The visual characteristics and growth rates of the electrical trees under ac, dc and impulse stresses were different. Partial discharge detection and analysis was used to measure the inception voltage and discharge activity at the conductor edge voids, to observe tree formation and growth, and to predict impending failure due to dielectric erosion. Electric field distributions were modeled and partial discharge inception levels were estimated from known void geometries. The staggered edge geometry appears to enhance the electric field stress at the recessed electrode.

Roll-to-roll compatible organic thin film transistor manufacturing technique by printing, lamination, and laser ablation

International Nuclear Information System (INIS)

Hassinen, Tomi; Ruotsalainen, Teemu; Laakso, Petri; Penttilä, Raimo; Sandberg, Henrik G.O.

2014-01-01

We present roll-to-roll printing compatible techniques for manufacturing organic thin film transistors using two separately processed foils that are laminated together. The introduction of heat-assisted lamination opens up possibilities for material and processing combinations. The lamination of two separately processed substrates together will allow usage of pre-patterned electrodes on both substrates and materials with non-compatible solvents. Also, the surface microstructure is formed differently when laminating dry films together compared to film formation from liquid phase. Demonstrator transistors, inverters and ring oscillators were produced using lamination techniques. Finally, a roll-to-roll compatible lamination concept is proposed where also the source and drain electrodes are patterned by laser ablation. The demonstrator transistors have shown very good lifetime in air, which is contributed partly to the good material combination and partly to the enhanced interface formation in heat-assisted lamination process. - Highlights: • A roll-to-roll compatible lamination technique for printed electronics is proposed. • Laser ablation allows highly defined metal top and bottom electrodes. • Method opens up processing possibilities for incompatible materials and solvents. • Shearing forces may enhance molecular orientation and packing. • An air stable polymer transistor is demonstrated with a lifetime of years

Minimality of invariant laminations for partially hyperbolic attractors

International Nuclear Information System (INIS)

Nobili, Felipe

2015-01-01

Let f : M → M be a C 1 -diffeomorphism over a compact boundaryless Riemannian manifold M, and Λ a compact f-invariant subset of M admitting a partially hyperbolic spliting T f Λ = E s  ⊕ E c  ⊕ E u over the tangent bundle T f Λ. It's known from the Hirsch–Pugh–Shub theory that Λ admits two invariant laminations associated to the extremal bundles E s and E u . These laminations are families of dynamically defined immersed submanifolds of the M tangent, respectively, to the bundles E s and E u at every point in Λ. In this work, we prove that at least one of the invariant laminations of a transitive partially hyperbolic attractor with a one-dimensional center bundle is minimal: the orbit of every leaf intersects Λ densely. This result extends those in Bonatti et al (2002 J. Inst. Math. Jussieu 1 513–41) and Hertz et al (2007 Fields Institute Communications vol 51 (Providence, RI: American Mathematical Society) pp 103–9) about minimal foliations for robustly transitive diffeomorphisms. (paper)

Equivalent circuit and optimum design of a multilayer laminated piezoelectric transformer.

Science.gov (United States)

Dong, Shuxiang; Carazo, Alfredo Vazquez; Park, Seung Ho

2011-12-01

A multilayer laminated piezoelectric Pb(Zr(1-x)Ti(x))O(3) (PZT) ceramic transformer, operating in a half- wavelength longitudinal resonant mode (λ/2 mode), has been analyzed. This piezoelectric transformer is composed of one thickness-polarized section (T-section) for exciting the longitudinal mechanical vibrations, two longitudinally polarized sections (L-section) for generating high-voltage output, and two insulating layers laminated between the T-section and L-section layers to provide insulation between the input and output sections. Based on the piezoelectric constitutive and motion equations, an electro-elasto-electric (EEE) equivalent circuit has been developed, and correspondingly, an effective EEE coupling coefficient was proposed for optimum design of this multilayer transformer. Commercial finite element analysis software is used to determine the validity of the developed equivalent circuit. Finally, a prototype sample was manufactured and experimental data was collected to verify the model's validity.

Laminated electrospun nHA/PHB-composite scaffolds mimicking bone extracellular matrix for bone tissue engineering

Energy Technology Data Exchange (ETDEWEB)

Chen, Zhuoyue [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Song, Yue [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Zhang, Jing [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Ministry of Education, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province, 710069 (China); Liu, Wei [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Cui, Jihong, E-mail: cjh@nwu.edu.cn [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Ministry of Education, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province, 710069 (China); and others

2017-03-01

Electrospinning is an effective means to generate nano- to micro-scale polymer fibers resembling native extracellular matrix for tissue engineering. However, a major problem of electrospun materials is that limited pore size and porosity may prevent adequate cellular infiltration and tissue ingrowth. In this study, we first prepared thin layers of hydroxyapatite nanoparticle (nHA)/poly-hydroxybutyrate (PHB) via electrospinning. We then laminated the nHA/PHB thin layers to obtain a scaffold for cell seeding and bone tissue engineering. The results demonstrated that the laminated scaffold possessed optimized cell-loading capacity. Bone marrow mesenchymal stem cells (MSCs) exhibited better adherence, proliferation and osteogenic phenotypes on nHA/PHB scaffolds than on PHB scaffolds. Thereafter, we seeded MSCs onto nHA/PHB scaffolds to fabricate bone grafts. Histological observation showed osteoid tissue formation throughout the scaffold, with most of the scaffold absorbed in the specimens 2 months after implantation, and blood vessels ingrowth into the graft could be observed in the graft. We concluded that electrospun and laminated nanoscaled biocomposite scaffolds hold great therapeutic potential for bone regeneration. - Highlights: • We laminated the nHA/PHB layers to obtain a scaffold for bone tissue engineering. • The laminated scaffold performed optimized cell-loading capacity. • MSCs exhibited osteogenic phenotypes on the laminated scaffold. • Osteoid tissue formed throughout the laminated scaffold after 2 months in vivo. The laminated bio-composite scaffolds can be applied to bone regeneration.

Laminated electrospun nHA/PHB-composite scaffolds mimicking bone extracellular matrix for bone tissue engineering

International Nuclear Information System (INIS)

Chen, Zhuoyue; Song, Yue; Zhang, Jing; Liu, Wei; Cui, Jihong

2017-01-01

Electrospinning is an effective means to generate nano- to micro-scale polymer fibers resembling native extracellular matrix for tissue engineering. However, a major problem of electrospun materials is that limited pore size and porosity may prevent adequate cellular infiltration and tissue ingrowth. In this study, we first prepared thin layers of hydroxyapatite nanoparticle (nHA)/poly-hydroxybutyrate (PHB) via electrospinning. We then laminated the nHA/PHB thin layers to obtain a scaffold for cell seeding and bone tissue engineering. The results demonstrated that the laminated scaffold possessed optimized cell-loading capacity. Bone marrow mesenchymal stem cells (MSCs) exhibited better adherence, proliferation and osteogenic phenotypes on nHA/PHB scaffolds than on PHB scaffolds. Thereafter, we seeded MSCs onto nHA/PHB scaffolds to fabricate bone grafts. Histological observation showed osteoid tissue formation throughout the scaffold, with most of the scaffold absorbed in the specimens 2 months after implantation, and blood vessels ingrowth into the graft could be observed in the graft. We concluded that electrospun and laminated nanoscaled biocomposite scaffolds hold great therapeutic potential for bone regeneration. - Highlights: • We laminated the nHA/PHB layers to obtain a scaffold for bone tissue engineering. • The laminated scaffold performed optimized cell-loading capacity. • MSCs exhibited osteogenic phenotypes on the laminated scaffold. • Osteoid tissue formed throughout the laminated scaffold after 2 months in vivo. The laminated bio-composite scaffolds can be applied to bone regeneration.

Vibration and Energy Dissipation of Nanocomposite Laminates for Below Ballistic Impact Loading

Directory of Open Access Journals (Sweden)

G. Balaganesan

Full Text Available Abstract Composite laminates are made of glass woven roving mats of 610gsm, epoxy resin and nano clay which are subjected to projectile impact. Nano clay dispersion is varied from 1% to 5%. Impact tests are conducted in a gas gun setup with a spherical nose cylindrical projectile of diameter 9.5 mm of mass 7.6 g. The energy absorbed by the laminates when subjected to impact loading is studied, the velocity range is below ballistic limit. The effect of nano clay on energy absorption in vibration, delamination and matrix crack is studied for different weight % of nano clay and for different thickness values of the laminates. The natural frequencies and damping factors are obtained for the laminates during impact and the effect of nano clay is studied. The results show considerable improvement in energy absorption due to the presence of nano clay

Laminated multilayer sheet structure and its utilization

International Nuclear Information System (INIS)

Chiba, K.; Itoh, K.; Mitani, Y.; Sobajima, S.; Yonemura, U.

1980-01-01

A laminated multilayer sheet structure is described comprising (A) an opaque flexible sheet layer, and (B) a flexible layer laminated on the surface of layer (A) and composed of a transparent thermic ray reflecting layer (B 1 ) bonded to a transparent synthetic resin layer (B 2 ), said layer (B 1 ) being a transparent thermic ray reflecting layer composed of (I) a layer of a metal having a thickness of about 50 to about 600 A, said metal being selected from the group consisting of gold, silver, copper, aluminum and a mixture of alloy of at least two of said metals, and (II) a high refractive substance layer having a thickness of about 50 to about 600 A, of an oxide of titanium derived from a layer of an organic titanium compound of the formula Ti 1 O/sub m/R/sub n/, where R is alkyl of 1-20 carbon atoms, l=1-30, m=4+3(1-1), and n=4+2(1-1), and containing the organic residual moiety of the organic titanium compound, the amount of said organic residual moiety being 0.1 to 30% by weight based on the weight of the high refractive substance layer; or said layer (B 1 ) being a transparent semiconductive layer having a thickness of about 500 to about 5,000 a and being composed of a compound selected from the group consisting of indium oxide, tin oxide, cadmium oxide, antimony oxide, copper iodide, and a mixture of at least two of said compounds. A method is described for heat-insulating a room, which comprises applying to the surface of a floor, wall, ceiling or partition in the room a laminated multilayer sheet structure comprising (A) an opaque flexible sheet layer, and (B) a flexible layer laminated on the surface of layer (A) and composed of a transparent thermic ray reflecting layer (B 1 ) bonded to a transparent synthetic resin layer

An Investigation on Tensile Properties of Glass Fiber/Aluminium Laminates

Directory of Open Access Journals (Sweden)

M. Sadighi

2009-12-01

Full Text Available The idea of combining low weight and good mechanical properties has led to efforts to develop a new light fiber/metal laminate (FML in the last decade. FMLs are hybrid composites consisting of alternating thin layers of metal sheets and fiber-reinforced epoxy prepregs. In this study, the effect of fiber orientation on tensile properties of this material is investigated both analytically and experimentally. An analytical constitutive model based on classical lamination theory by using Kirchhoff-Love assumption, which incorporates the elastic-plastic behavior of the aluminium alloy was applied. Test results show that fiber sheet, with zero angle in laminates, improve the tensile strength. The composite layers with different fiber orientation change specimens' mode of fracture. Good agreement is obtained between the model predictions and experimental results.

Experimental and Numerical Study on the Tensile Behaviour of UACS/Al Fibre Metal Laminate

Science.gov (United States)

Xue, Jia; Wang, Wen-Xue; Zhang, Jia-Zhen; Wu, Su-Jun; Li, Hang

2015-10-01

A new fibre metal laminate fabricated with aluminium sheets and unidirectionally arrayed chopped strand (UACS) plies is proposed. The UACS ply is made by cutting parallel slits into a unidirectional carbon fibre prepreg. The UACS/Al laminate may be viewed as aluminium laminate reinforced by highly aligned, discontinuous carbon fibres. The tensile behaviour of UACS/Al laminate, including thermal residual stress and failure progression, is investigated through experiments and numerical simulation. Finite element analysis was used to simulate the onset and propagation of intra-laminar fractures occurring within slits of the UACS plies and delamination along the interfaces. The finite element models feature intra-laminar cohesive elements inserted into the slits and inter-laminar cohesive elements inserted at the interfaces. Good agreement are obtained between experimental results and finite element analysis, and certain limitations of the finite element models are observed and discussed. The combined experimental and numerical studies provide a detailed understanding of the tensile behaviour of UACS/Al laminates.

A Progressive Damage Model for Predicting Permanent Indentation and Impact Damage in Composite Laminates

Science.gov (United States)

Ji, Zhaojie; Guan, Zhidong; Li, Zengshan

2017-10-01

In this paper, a progressive damage model was established on the basis of ABAQUS software for predicting permanent indentation and impact damage in composite laminates. Intralaminar and interlaminar damage was modelled based on the continuum damage mechanics (CDM) in the finite element model. For the verification of the model, low-velocity impact tests of quasi-isotropic laminates with material system of T300/5228A were conducted. Permanent indentation and impact damage of the laminates were simulated and the numerical results agree well with the experiments. It can be concluded that an obvious knee point can be identified on the curve of the indentation depth versus impact energy. Matrix cracking and delamination develops rapidly with the increasing impact energy, while considerable amount of fiber breakage only occurs when the impact energy exceeds the energy corresponding to the knee point. Predicted indentation depth after the knee point is very sensitive to the parameter μ which is proposed in this paper, and the acceptable value of this parameter is in range from 0.9 to 1.0.

The shock response of float-glass laminates

International Nuclear Information System (INIS)

Bourne, N.K.

2005-01-01

Interfaces within glass targets give rise to variations in the mode of failure of material components. The wide use of such laminates merits further investigation of the failure mechanism. It is already known that when shocked above a threshold of 4 GPa, glass fails under compression behind a propagating front following the compression front. Work is presented which indicates how this failure process is altered by bonding together two plates to introduce an interface, rather than leaving a monolithic target. After crossing an internal interface, the failure wave propagates only after a delay in soda-lime glass and the failed strength of the material is increased at the inner interface compared with that at the impact face. Addition of a second interface illustrates these effects. Recent work has shown that failure of more than two plates bonded together during impact shapes the pulse transmitted through materials. Indeed it has been suggested that glass sheets bonded together show some of the features of polycrystalline brittle materials. In this work, the stress has been monitored at different stations in the laminate to ascertain the effect of varying the number of tiles within the laminate. It is found that the pulse rises to ca. 4 GPa quickly and then is ramped more gradually as the number of glass sheets is increased

Damage in woven CFRP laminates subjected to low velocity impacts

International Nuclear Information System (INIS)

Ullah, H; Abdel-Wahab, A A; Harland, A R; Silberschmidt, V V

2012-01-01

Carbon fabric-reinforced polymer (CFRP) composites used in sports products can be exposed to different in-service conditions such as large dynamic bending deformations caused by impact loading. Composite materials subjected to such loads demonstrate various damage modes such as matrix cracking, delamination and, ultimately, fabric fracture. Damage evolution in these materials affects both their in-service properties and performance that can deteriorate with time. These processes need adequate means of analysis and investigation, the major approaches being experimental characterisation and non-destructive examination of internal damage in composite laminates. This research deals with a deformation behaviour and damage in woven composite laminates due to low-velocity dynamic out-of-plane bending. Experimental tests are carried out to characterise the behaviour of such laminates under large-deflection dynamic bending in un-notched specimens in Izod tests using a Resil Impactor. A series of low-velocity impact tests is carried out at various levels of impact energy to assess the energy absorbed and force-time response of CFRP laminates. X-ray micro computed tomography (micro-CT) is used to investigate material damage modes in the impacted specimens. X-ray tomographs revealed that through-thickness matrix cracking, inter-ply delamination and intra-ply delamination, such as tow debonding and fabric fracture, were the prominent damage modes.

Boride-based nano-laminates with MAX-phase-like behaviour