Detection of a novel highly pathogenic H7 influenza virus by duplex real-time reverse transcription polymerase chain reaction.

1. National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, PR China; Key Laboratory of Zoonoses, Ministry of Agriculture, Guangzhou, 510642, PR China.
Authors
Jia W¹
Xie S¹
Wang X¹
Dai Y¹
Li Z¹
Niu X¹
Qi W¹
Liao M¹
(8 authors)
2. Animal and Plant Inspection and Quarantine Technology Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, 518054, PR China.
Authors
Cao C²
Lin Y²
Lu T²
(3 authors)
3. Animal Disease Control Center of Dongguan City, Dongguan, 523086, PR China.
Authors
Zhong L³
Yin S³
Xu Z³
(3 authors)

ORCIDs linked to this article

Journal of Virological Methods, 12 Apr 2017, 246:100-103
https://doi.org/10.1016/j.jviromet.2017.03.014 PMID: 28411129

Abstract

On February 19, 2017, China announced that the mutant H7N9 virus appeared in human cases, which showed molecular characteristic of highly pathogenic virus for poultry. In this study, a duplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay was developed for distinguish between highly pathogenic H7 virus and low pathogenic H7 virus. The sensitivity, specificity, stability and conformance tests were conducted for this method. The data showed that the new method is sensitive. The minimum detection limit for the RNA of highly pathogenic H7 virus is 0.0052fg and the minimum detection limit for the RNA of low pathogenic H7 virus is 0.36fg. The method gave specific results in detecting novel highly pathogenic H7 virus and will play an important role in the rapid identification of novel highly pathogenic H7 virus.

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Funding

Funders who supported this work.

National Key Research Projects (1)

Grant ID: 2016YFD0500800
1 publication

National Science and Technology Basic Work Special Projects (1)

Grant ID: 2012FY111000
1 publication

Program for National Broiler Industry (1)

Grant ID: CARS-42-G09
1 publication

Science and Technology Planning Projects of Guangdong Province (1)

Grant ID: 2013B020224
1 publication

Science and Technology Projects of Dongguan (1)

Grant ID: 2014108101041
1 publication

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Detection of a novel highly pathogenic H7 influenza virus by duplex real-time reverse transcription polymerase chain reaction.

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Avian influenza A(N9) virus and mixed live poultry–animal markets in Guangdong province: a perfect storm in the making?

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Funding

National Key Research Projects (1)﻿

National Science and Technology Basic Work Special Projects (1)﻿

Program for National Broiler Industry (1)﻿

Science and Technology Planning Projects of Guangdong Province (1)﻿

Science and Technology Projects of Dongguan (1)﻿

National Key Research Projects (1)

National Science and Technology Basic Work Special Projects (1)

Program for National Broiler Industry (1)

Science and Technology Planning Projects of Guangdong Province (1)

Science and Technology Projects of Dongguan (1)