Sample records for zytokinfreisetzung unter n-acetylcystein

  1. N-acetylcysteine increased rice yield




    N-acetylcysteine (NAC) biosynthesized reduced glutathione (GSH), which maintains redox homeostasis in plants under normal and stressful conditions. To justify the effects of NAC on rice production, we measured yield parameters, chlorophyll (Chl) content, minimum Chl fluorescence (Fo), maximum Chl fluorescence (Fm), quantum yield (Fv/Fm), net photosynthesis rate (Pn), photosynthetically active radiation (PAR), and relative water content (RWC). Four treatments, N1G0 (nitrogen (N) with no NAC), ...

  2. N-Acetylcysteine Reverses Cocaine Induced Metaplasticity (United States)

    Moussawi, Khaled; Pacchioni, Alejandra; Moran, Megan; Olive, M. Foster; Gass, Justin T.; Lavin, Antonieta; Kalivas, Peter W


    Cocaine addiction is characterized by an impaired ability to develop adaptive behaviors that can compete with cocaine seeking, implying a deficit in the ability to induce plasticity in cortico-accumbens circuitry critical for regulating motivated behavior. RWe found that rats withdrawn from cocaine self-administration had a marked in vivo deficit in the ability to develop long-term potentation (LTP) and depression (LTD) in the nucleus accumbens core subregion following stimulation of prefrontal cortex. N-acetylcysteine treatment prevents relapse in animal models and craving in humans by activating cystine-glutamate exchange and thereby stimulating extrasynaptic metabotropic glutamate receptors (mGluR). N-acetylcysteine treatment restored the ability to induce LTP and LTD by indirectly stimulating mGluR2/3 and mGluR5, respectively. Cocaine self-administration induces metaplasticity that inhibits the further induction of synaptic plasticity, and this impairment can be reversed by N-acetylcysteine, a drug that also prevents relapse. PMID:19136971

  3. Status epilepticus following intravenous N-acetylcysteine therapy. (United States)

    Hershkovitz, E; Shorer, Z; Levitas, A; Tal, A


    A previously healthy 2 1/2-year-old girl developed status epilepticus followed by cortical blindness during intravenous N-acetylcysteine therapy for paracetamol ingestion. The child's vision was almost completely recovered during the 18 months follow-up period. We assume that the cortical blindness was a postictal sequela after prolonged seizure episode, most probably due to respiratory depression induced by N-acetylcysteine.

  4. N-acetylcysteine for treatment of autism, a case report

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    Ahmad Ghanizadeh


    Full Text Available There are a limited number of Food and Drug Administration (FDA-approved medications for the treatment of autism. Meanwhile, oxidative stress and neuroinflammation are supposed to play a causative role in autism. N-acetylcysteine may provide cystine, a precursor for glutathione (GSH, which is an important antioxidant factor in the brain. We here report a child with autism, whose symptoms were markedly decreased after taking oral N-acetylcysteine 800 mg/day, in three divided doses. His social interaction was significantly increased. The score of social impairment on a visual analog scale decreased from 10 to 6 in the two-month trial. The aggressive behaviors decreased from 10 to 3. This case suggests that N-acetylcysteine may decrease some symptoms of autism.

  5. N-Acetylcysteine in the prevention of ototoxicity

    DEFF Research Database (Denmark)

    Tepel, Martin


    Prevention of ototoxicity after the administration of aminoglycoside antibiotics has been notably difficult, in particular in patients with chronic kidney disease. Feldman et al. report that oral administration of 600 mg N-acetylcysteine twice daily significantly ameliorates gentamicin-induced ot......-induced ototoxicity in hemodialysis patients. That approach may help to prevent aminoglycoside-induced hearing loss in these high-risk patients in daily practice.......Prevention of ototoxicity after the administration of aminoglycoside antibiotics has been notably difficult, in particular in patients with chronic kidney disease. Feldman et al. report that oral administration of 600 mg N-acetylcysteine twice daily significantly ameliorates gentamicin...

  6. Recommendations for the paracetamol treatment nomogram and side effects of N-acetylcysteine

    NARCIS (Netherlands)

    Koppen, A.; van Riel, A.; de Vries, I.; Meulenbelt, J.


    Treatment of paracetamol intoxication consists of administration of N-acetylcysteine, preferably shortly after paracetamol ingestion. In most countries, the decision to treat patients with N-acetylcysteine depends on the paracetamol plasma concentration. In the literature, different arguments are

  7. Systematic review of N-acetylcysteine in cystic fibrosis

    NARCIS (Netherlands)

    Duijvestijn, YCM; Brand, PLP

    A systematic review was carried out to evaluate whether the use of N-acetylcysteine to improve lung function in patients with cystic fibrosis is supported by published evidence. Medline and the Cochrane Library were searched and the reference lists of all retrieved papers and of relevant chapters of

  8. Effect of intravenous N-acetylcysteine infusion on haemostatic parameters in healthy subjects

    DEFF Research Database (Denmark)

    Knudsen, TT; Thorsen, S; Jensen, SA


    BACKGROUND AND AIMS: N-acetylcysteine is used to treat paracetamol overdose but depresses the activity of plasma coagulation factors II, VII, and X, which are often used to assess liver injury. The aim of this study was to investigate the effect of N-acetylcysteine on haemostasis in normal...... volunteers. METHODS: Haemostatic parameters in 10 healthy subjects were analysed before and following intravenous infusion of therapeutic doses of N-acetylcysteine, as well as in vitro. RESULTS: N-acetylcysteine induced significant decreases in plasma levels of vitamin K dependent haemostatic proteins...... activity, and free protein S reactivity, respectively. These data suggest that N-acetylcysteine induces protein modifications affecting activity. Five subjects developed an adverse reaction to infusion of N-acetylcysteine and these were associated with a rapid increase in levels of factor VIII and its...

  9. Evaluation of N-acetylcysteine for the prevention of contrast-induced nephropathy

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    Sara K. Richter


    Full Text Available Background: Contrast-induced nephropathy (CIN remains a leading cause of acute renal failure in hospitalized patients. N-Acetylcysteine has been studied previously for the prevention of CIN, resulting in mixed findings. Objective: The objective of this study was to determine the impact of N-acetylcysteine on the development of CIN in order to guide its use at community, teaching hospitals. Methods: Patients admitted between January 1 and December 31, 2011, receiving intravenous radiocontrast dye were included if they were compliant with two or more of the following conditions: baseline serum creatinine >1.2 mg/dL or estimated creatinine clearance <50 mL/min, age ≥75 years, diabetes mellitus, heart failure, or hypertension. The primary outcome was the difference in the proportion of patients in each group (N-acetylcysteine or no N-acetylcysteine who developed CIN, which was defined as a ≥0.5 mg/dL increase in serum creatinine or a ≥25% increase in serum creatinine within 12–96 hours post-exposure to contrast. Results: A total of 302 patients were included, 151 who received N-acetylcysteine and 151 who did not receive N-acetylcysteine. Patients who received N-acetylcysteine had significantly worse renal function at baseline than those who did not receive N-acetylcysteine (mean pre-contrast serum creatinine, 1.41 vs. 0.95 mg/dL, p<0.0001. A lower proportion of patients developing CIN was observed between those who received N-acetylcysteine and those who did not receive N-acetylcysteine (10.2% vs. 21.8%, p=0.0428. Conclusions: The use of N-acetylcysteine was likely associated with a reduced incidence of CIN in patients at risk for CIN development. Based on these results, hospitals may benefit from the development of a protocol to guide the appropriate use of N-acetylcysteine.

  10. The promise of N-acetylcysteine in neuropsychiatry. (United States)

    Berk, Michael; Malhi, Gin S; Gray, Laura J; Dean, Olivia M


    N-Acetylcysteine (NAC) targets a diverse array of factors germane to the pathophysiology of multiple neuropsychiatric disorders including glutamatergic transmission, the antioxidant glutathione, neurotrophins, apoptosis, mitochondrial function, and inflammatory pathways. This review summarises the areas where the mechanisms of action of NAC overlap with known pathophysiological elements, and offers a précis of current literature regarding the use of NAC in disorders including cocaine, cannabis, and smoking addictions, Alzheimer's and Parkinson's diseases, autism, compulsive and grooming disorders, schizophrenia, depression, and bipolar disorder. There are positive trials of NAC in all these disorders, and although many of these require replication and are methodologically preliminary, this makes it one of the most promising drug candidates in neuropsychiatric disorders. The efficacy pattern of NAC interestingly shows little respect for the current diagnostic systems. Its benign tolerability profile, its action on multiple operative pathways, and the emergence of positive trial data make it an important target to investigate. Copyright © 2013 Elsevier Ltd. All rights reserved.

  11. N-Acetylcysteine reverses cocaine-induced metaplasticity. (United States)

    Moussawi, Khaled; Pacchioni, Alejandra; Moran, Megan; Olive, M Foster; Gass, Justin T; Lavin, Antonieta; Kalivas, Peter W


    Cocaine addiction is characterized by an impaired ability to develop adaptive behaviors that can compete with cocaine seeking, implying a deficit in the ability to induce plasticity in cortico-accumbens circuitry crucial for regulating motivated behavior. We found that rats withdrawn from cocaine self-administration had a marked in vivo deficit in the ability to develop long-term potentiation (LTP) and long-term depression (LTD) in the nucleus accumbens core subregion after stimulation of the prefrontal cortex. N-acetylcysteine (NAC) treatment prevents relapse in animal models and craving in humans by activating cystine-glutamate exchange and thereby stimulating extrasynaptic metabotropic glutamate receptors (mGluR). NAC treatment of rats restored the ability to induce LTP and LTD by indirectly stimulating mGluR2/3 and mGluR5, respectively. Our findings show that cocaine self-administration induces metaplasticity that inhibits further induction of synaptic plasticity, and this impairment can be reversed by NAC, a drug that also prevents relapse.

  12. Interactive effects of N-acetylcysteine and antidepressants. (United States)

    Costa-Campos, Luciane; Herrmann, Ana P; Pilz, Luísa K; Michels, Marcus; Noetzold, Guilherme; Elisabetsky, Elaine


    N-acetylcysteine (NAC), a glutathione precursor and glutamate modulator, has been shown to possess various clinically relevant psychopharmacological properties. Considering the role of glutamate and oxidative stress in depressive states, the poor effectiveness of antidepressant drugs (ADs) and the benefits of drug combination for treating depression, the aim of this study was to explore the possible benefit of NAC as an add on drug to treat major depression. For that matter we investigated the combination of subeffective and effective doses of NAC with subeffective and effective doses of several ADs in the mice tail suspension test. The key finding of this study is that a subeffective dose of NAC reduced the minimum effective doses of imipramine and escitalopram, but not those of desipramine and bupropion. Moreover, the same subeffective dose of NAC increased the minimum effective dose of fluoxetine in the same model. In view of the advantages associated with using the lowest effective dose of antidepressant, the results of this study suggest the potential of a clinically useful interaction of NAC with imipramine and escitalopram. Further studies are necessary to better characterize the molecular basis of such interactions, as well as to typify the particular drug combinations that would optimize NAC as an alternative for treating depression. Copyright © 2013 Elsevier Inc. All rights reserved.

  13. N-acetylcysteine for major depressive episodes in bipolar disorder. (United States)

    Magalhães, Pedro V; Dean, Olívia M; Bush, Ashley I; Copolov, David L; Malhi, Gin S; Kohlmann, Kristy; Jeavons, Susan; Schapkaitz, Ian; Anderson-Hunt, Murray; Berk, Michael


    In this report, we aimed to evaluate the effect of add-on N-acetylcysteine (NAC) on depressive symptoms and functional outcomes in bipolar disorder. To that end, we conducted a secondary analysis of all patients meeting full criteria for a depressive episode in a placebo controlled trial of adjunctive NAC for bipolar disorder. Twenty-four week randomised clinical trial comparing adjunctive NAC and placebo in individuals with bipolar disorder experiencing major depressive episodes. Symptomatic and functional outcome data were collected over the study period. Seventeen participants were available for this report. Very large effect sizes in favor of NAC were found for depressive symptoms and functional outcomes at endpoint. Eight of the ten participants on NAC had a treatment response at endpoint; the same was true for only one of the seven participants allocated to placebo. These results indicate that adjunctive NAC may be useful for major depressive episodes in bipolar disorder. Further studies designed to confirm this hypothesis are necessary.

  14. N-acetylcysteine prevents ketamine-induced adverse effects on development, heart rate and monoaminergic neurons in zebrafish. (United States)

    Robinson, Bonnie; Dumas, Melanie; Gu, Qiang; Kanungo, Jyotshna


    N-acetylcysteine, a precursor molecule of glutathione, is an antioxidant. Ketamine, a pediatric anesthetic, has been implicated in cardiotoxicity and neurotoxicity including modulation of monoaminergic systems in mammals and zebrafish. Here, we show that N-acetylcysteine prevents ketamine's adverse effects on development and monoaminergic neurons in zebrafish embryos. The effects of ketamine and N-acetylcysteine alone or in combination were measured on the heart rate, body length, brain serotonergic neurons and tyrosine hydroxylase-immunoreactive (TH-IR) neurons. In the absence of N-acetylcysteine, a concentration of ketamine that produces an internal embryo exposure level comparable to human anesthetic plasma concentrations significantly reduced heart rate and body length and those effects were prevented by N-acetylcysteine co-treatment. Ketamine also reduced the areas occupied by serotonergic neurons in the brain, whereas N-acetylcysteine co-exposure counteracted this effect. TH-IR neurons in the embryo brain and TH-IR cells in the trunk were significantly reduced with ketamine treatment, but not in the presence of N-acetylcysteine. In our continued search for compounds that can prevent ketamine toxicity, this study using specific endpoints of developmental toxicity, cardiotoxicity and neurotoxicity, demonstrates protective effects of N-acetylcysteine against ketamine's adverse effects. This is the first study that shows the protective effects of N-acetylcysteine on ketamine-induced developmental defects of monoaminergic neurons as observed in a whole organism. Published by Elsevier B.V.

  15. A gargantuan acetaminophen level in an acidemic patient treated solely with intravenous N-acetylcysteine. (United States)

    Zell-Kanter, Michele; Coleman, Patrick; Whiteley, Patrick M; Leikin, Jerrold B


    The objective of this report is to describe an acidemic patient with one of the largest recorded acetaminophen ingestions in a patient with acidemia who was treated with supportive care and intravenous (IV) N-acetylcysteine. A 59-year-old female with a history of depression was found comatose. In the Emergency Department, she was obtunded with agonal respirations and immediately intubated. Activated charcoal was given through a nasogastric tube. An initial acetaminophen serum level was 1141 mg/L. The patient was started on IV N-acetylcysteine. The acetaminophen level peaked 2 hours later at 1193 mg/L. She was continued on the IV N-acetylcysteine protocol. The next day her aspartate aminotransferase was 3150 U/L, alanine aminotransferase was 2780 U/L, and creatinine phosphokinase was 16,197 U/L. There was no elevation in bilirubin or international normalized ratio (INR). Transaminase levels decreased on day 3 and normalized by day 4 when she was transferred to a psychiatric unit. Few cases have been reported of strikingly elevated acetaminophen levels in poisoned patients who did not receive hemodialysis. These patients did have increased lactate levels, and some had normal liver function tests. All of these patients received N-acetylcysteine and survived the poisoning without sequelae. This patient in this report was unique in that she had the highest reported serum acetaminophen level with acidosis and was treated successfully with only IV N-acetylcysteine and supportive care.

  16. Determination of N-acetylcysteine via its effect on the aggregation of gold nanoparticles

    International Nuclear Information System (INIS)

    Sierra-Rodero, M.; Fernandez-Romero, J.M.; Gomez-Hens, A.


    The effect of thiol compounds on the kinetics of the aggregation of gold nanoparticles in the presence of the cationic surfactant cetyltrimethyl ammonium bromide has been studied. It was applied to the determination of N-acetylcysteine using the stopped-flow mixing technique along with light scattering detection. The signal obtained was measured after about 5 s, and gave the analytical information for a calibration graph in the concentration range from 2.9 to 60 μmol L -1 of N-acetylcysteine, and a detection limit of 0.87 μmol L- 1. The effect of other thiols on the system is also described. The relative standard deviation ranges between 0.6% and 3.5%. The method was applied to the determination of N-acetylcysteine in several pharmaceutical samples with recoveries that range from 97.7% to 101.1% (author)

  17. N-acetylcysteine enhances nitroglycerin-induced headache and cranial arterial responses

    DEFF Research Database (Denmark)

    Iversen, Helle Klingenberg


    The effects of N-acetylcysteine, a sulfhydryl group donor, on nitroglycerin-induced headache and dilation of temporal and radial arteries were investigated in 11 healthy volunteers. Nitroglycerin, 0.06 microgram/kg/min, was infused for 20 minutes immediately after and 120 minutes after pretreatment...... response (median headache score, 3 versus 1), and the headache retained its vascular characteristics. Temporal artery dilation was also potentiated by N-acetylcysteine, 139% +/- 3% versus 127% +/- 3% of baseline, whereas the radial artery was unaffected. The potentiation was most pronounced after the first...... nitroglycerin infusion (12% versus 4.5% compared with placebo). A prolonged dilation of the temporal artery was observed only after the first nitroglycerin infusion, when high levels of N-acetylcysteine were present....

  18. N-acetylcysteine for sepsis and systemic inflammatory response in adults. (United States)

    Szakmany, Tamas; Hauser, Balázs; Radermacher, Peter


    Death is common in systemic inflammatory response syndrome (SIRS) or sepsis-induced multisystem organ failure and it has been thought that antioxidants such as N-acetylcysteine could be beneficial. We assessed the clinical effectiveness of intravenous N-acetylcysteine for the treatment of patients with SIRS or sepsis. We searched the following databases: Cochrane Central Register of Clinical Trials (CENTRAL) (The Cochrane Library 2011, Issue 12); MEDLINE (January 1950 to January 2012); EMBASE (January 1980 to January 2012); CINAHL (1982 to January 2012); the NHS Trusts Clinical Trials Register and Current Controlled Trials (; LILACS; KoreaMED; MEDCARIB; INDMED; PANTELEIMON; Ingenta; ISI Web of Knowledge and the National Trials Register to identify all relevant randomized controlled trials available for review. We included only randomized controlled trials (RCTs) in the meta-analysis. We independently performed study selection, quality assessment and data extraction. We estimated risk ratios (RR) for dichotomous outcomes. We measured statistical heterogeneity using the I(2) statistic. We included 41 fully published studies (2768 patients). Mortality was similar in the N-acetylcysteine group and the placebo group (RR 1.06, 95% CI 0.79 to 1.42; I(2) = 0%). Neither did N-acetylcysteine show any significant effect on length of stay, duration of mechanical ventilation or incidence of new organ failure. Early application of N-acetylcysteine to prevent the development of an oxidato-inflammatory response did not affect the outcome, nor did late application that is after 24 hours of developing symptoms. Late application was associated with cardiovascular instability. Overall, this meta-analysis puts doubt on the safety and utility of intravenous N-acetylcysteine as an adjuvant therapy in SIRS and sepsis. At best, N-acetylcysteine is ineffective in reducing mortality and complications in this patient population. At worst, it can be harmful

  19. Evaluation of topical n-acetylcysteine in diversion colitis

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    Marcos Gonçalves de Almeida


    Full Text Available INTRODUCTION: Diversion colitis (DC is a benign condition characterized by the appearance of inflammation in the mucosa of the colon or rectum devoid of fecal stream. Oxidative stress has been associated with the etiopathogenesis of the disease. N-acetylcysteine (NAC is a substance with antioxidant properties, used in different treatments of inflammatory diseases. The purpose of this study was to evaluate the effects of topical applications of NAC in an experimental model of DC. METHODS: Thirty-six Wistar rats were submitted to deviation of fecal stream by proximal colostomy and a distal mucosal fistula. They were distributed into 3 experimental groups of 12 animals according to the daily application of enemas containing 0.9% saline or 2 doses of NAC, 25 mg/kg and 100 mg/kg, respectively. In each group, half of the animals were sacrificed after two weeks of irrigation and half after four weeks of irrigation. The diagnosis of colitis was assessed by histopathological analysis and the grade of inflammation by inflammatory grading scale. The results were evaluated with the Mann-Whitney test, adopting significance level of 5% (pINTRODUÇÃO: Colite de exclusão (CE é uma condição benigna caracterizada pelo desenvolvimento de inflamação na mucosa do cólon desprovida de trânsito fecal. O estresse oxidativo tem sido implicado na patogênese da doença. A n-acetilcisteína (NAC é uma substância com efeitos antioxidantes, sendo utilizada no tratamento de várias doenças inflamatórias. OBJETIVO: Avaliar os efeitos da aplicação tópica de NAC em modelo de CE. MÉTODO: Trinta e seis ratos Wistar foram submetidos ao desvio do trânsito por meio de colostomia proximal e fístula mucosa distal. Os animais foram distribuídos em três grupos experimentais de igual tamanho segundo a aplicação de enemas diários contendo soro fisiológico 0,9% ou NAC nas concentrações de 25 mg/kg ou 100 mg/kg. Em cada grupo, metade dos animais foi sacrificada ap

  20. Antioxidant treatment with N-acetylcysteine during adult respiratory distress syndrome

    DEFF Research Database (Denmark)

    Jepsen, S; Herlevsen, P; Knudsen, P


    OBJECTIVE: To examine whether the antioxidant N-acetylcysteine could ameliorate the course of the adult respiratory distress syndrome (ARDS) in man. DESIGN: Randomized, double-blind, placebo-controlled study. SETTING: Medical and surgical ICU in a regional hospital. PATIENTS: Sixty-six ICU patients...

  1. Acute ethanol administration reduces the antidote effect of N-acetylcysteine after acetaminophen overdose in mice

    DEFF Research Database (Denmark)

    Dalhoff, K; Hansen, P B; Ott, P


    given ethanol or saline alone only 7% and 3%, respectively, survived 96 h. 4. The data suggest that the protective effect of N-acetylcysteine on acetaminophen-induced toxicity in fed mice is reduced by concomitant administration of ethanol. This may explain the clinical observation that ingestion...

  2. N-Acetylcysteine in depressive symptoms and functionality: a systematic review and meta-analysis. (United States)

    Fernandes, Brisa S; Dean, Olivia M; Dodd, Seetal; Malhi, Gin S; Berk, Michael


    To assess the utility of N-acetylcysteine administration for depressive symptoms in subjects with psychiatric conditions using a systematic review and meta-analysis. A computerized literature search was conducted in MEDLINE, Embase, the Cochrane Library, SciELO, PsycINFO, Scopus, and Web of Knowledge. No year or country restrictions were used. The Boolean terms used for the electronic database search were (NAC OR N-acetylcysteine OR acetylcysteine) AND (depression OR depressive OR depressed) AND (trial). The last search was performed in November 2014. The literature was searched for double-blind, randomized, placebo-controlled trials using N-acetylcysteine for depressive symptoms regardless of the main psychiatric condition. Using keywords and cross-referenced bibliographies, 38 studies were identified and examined in depth. Of those, 33 articles were rejected because inclusion criteria were not met. Finally, 5 studies were included. Data were extracted independently by 2 investigators. The primary outcome measure was change in depressive symptoms. Functionality, quality of life, and manic and anxiety symptoms were also examined. A full review and meta-analysis were performed. Standardized mean differences (SMDs) and odds ratios (ORs) with 95% CIs were calculated. Five studies fulfilled our inclusion criteria for the meta-analysis, providing data on 574 participants, of whom 291 were randomized to receive N-acetylcysteine and 283 to placebo. The follow-up varied from 12 to 24 weeks. Two studies included subjects with bipolar disorder and current depressive symptoms, 1 included subjects with MDD in a current depressive episode, and 2 included subjects with depressive symptoms in the context of other psychiatric conditions (1 trichotillomania and 1 heavy smoking). Treatment with N-acetylcysteine improved depressive symptoms as assessed by Montgomery-Asberg Depression Rating Scale and Hamilton Depression Rating Scale when compared to placebo (SMD = 0.37; 95% CI = 0

  3. The Effect of N-acetylcysteine on postoperative pain after laparoscopic cholecystectomy: a randomized clinical trial

    Directory of Open Access Journals (Sweden)

    Shahram Seyfi


    Full Text Available Background: Postoperative pain is one of the most common complications following laparoscopic cholecystectomy. Because the majority of the analgesic drugs including opioids and nonsteroidal anti-inflammatory drugs have many side effects, using drugs with lesser side effects is beneficial. The aim of this study was to evaluate the effect of N-acetylcysteine on the pain after laparoscopic cholecystectomy. Methods: In a randomized clinical trial, in two university-affiliated teaching hospitals in Babol City (Shahid Beheshti and Shahid Yahyanezhad Hospitals, Iran, from August 2015 to March 2015, a total number of 38 patients with age of 20-50 years, who were candidates for laparoscopic cholecystectomy with American Society of Anesthesiologists Class-I were chosen and randomly assigned into two groups. The night before operation, 1200 mg oral N-acetylcysteine is given to intervention group. Also, they received 600 mg IV N-acetylcysteine in the morning before operation. In the control group, two vitamin C effervescent tablets as placebo were given at night before operation and 3 ml sterile water as placebo was injected in the morning of operation. Amount of pethidine consumption and the changes in hemodynamic in two groups was recorded and analyzed at 24 hours after operation. Results: The average of patients age was not significant different between two groups (P=0.23. Average of pain score in placebo group was 3.5 and in N-acetylcysteine group was 2.7 that it was not significant difference between two groups (P=0.06. Average of pethidine consumption in placebo group was 52 mg and in N-acetylcysteine group was 29 mg in 24 hours, that the difference was statistically significant between two groups (P=0.01 Conclusion: As the results of the study, it can be concluded that the anti-inflammatory effects N- acetylcysteine can inhibit the function of lipoproteins and prostaglandins, reduced glutathione peroxidase and dismutase has been restored and can be

  4. Effect of N-acetylcysteine on the human nasal ciliary activity in vitro

    DEFF Research Database (Denmark)

    Stafanger, G; Bisgaard, H; Pedersen, M


    N-acetylcysteine (NAC) is widely used as a mucolytic agent, but the clinical and pharmacological effects of NAC are still unclear. It has recently been claimed in animal studies that NAC will stimulate ciliary beating frequency at low concentrations, while inhibiting beating at higher concentrati......N-acetylcysteine (NAC) is widely used as a mucolytic agent, but the clinical and pharmacological effects of NAC are still unclear. It has recently been claimed in animal studies that NAC will stimulate ciliary beating frequency at low concentrations, while inhibiting beating at higher...... concentrations. Using a microphoto-oscillographic method combined with microperfusion technique, we studied the direct effect of NAC on human nasal cilia. NAC caused a direct dose- and time-related decrease in ciliary beating frequency, which was detectable at 2 mg/ml and reached statistically significant levels...

  5. The effect of N-acetylcysteine and melatonin in adult SHR with established hypertension

    Czech Academy of Sciences Publication Activity Database

    Pecháňová, O.; Zicha, Josef; Paulis, L.; Kojšová, S.; Jendeková, L.; Dobešová, Zdenka; Sládková, M.; Šimko, F.; Kuneš, Jaroslav


    Roč. 48, č. 4 (2006), s. 776-777 ISSN 0194-911X. [Annual Meeting of the European Council for Cardiovascular Research (ECCR) /11./. 29.09.2006-01.10.2006, La Colle sur Loup] Grant - others:VEGA(SK) 2/6148/26; VEGA(SK) 1/3429/06; VEGA(SK) 1/3442/06 Keywords : N-acetylcysteine * melatonin * SHR * hypertension Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery

  6. Acute Chloroform Ingestion Successfully Treated with Intravenously Administered N-acetylcysteine


    Dell’Aglio, Damon M.; Sutter, Mark E.; Schwartz, Michael D.; Koch, David D.; Algren, D. A.; Morgan, Brent W.


    Chloroform, a halogenated hydrocarbon, causes central nervous system depression, cardiac arrhythmias, and hepatotoxicity. We describe a case of chloroform ingestion with a confirmatory serum level and resultant hepatotoxicity successfully treated with intravenously administered N-acetylcysteine (NAC). A 19-year-old man attempting suicide ingested approximately 75 mL of chloroform. He was unresponsive and intubated upon arrival. Intravenously administered NAC was started after initial stabiliz...

  7. N-Acetylcysteine reduces cocaine-cue attentional bias and differentially alters cocaine self-administration based on dosing order. (United States)

    Levi Bolin, B; Alcorn, Joseph L; Lile, Joshua A; Rush, Craig R; Rayapati, Abner O; Hays, Lon R; Stoops, William W


    Disrupted glutamate homeostasis is thought to contribute to cocaine-use disorder, in particular, by enhancing the incentive salience of cocaine stimuli. n-Acetylcysteine might be useful in cocaine-use disorder by normalizing glutamate function. In prior studies, n-acetylcysteine blocked the reinstatement of cocaine seeking in laboratory animals and reduced the salience of cocaine stimuli and delayed relapse in humans. The present study determined the ability of maintenance on n-acetylcysteine (0 or 2400mg/day, counterbalanced) to reduce the incentive salience of cocaine stimuli, as measured by an attentional bias task, and attenuate intranasal cocaine self-administration (0, 30, and 60mg). Fourteen individuals (N=14) who met criteria for cocaine abuse or dependence completed this within-subjects, double-blind, crossover-design study. Cocaine-cue attentional bias was greatest following administration of 0mg cocaine during placebo maintenance, and was attenuated by n-acetylcysteine. Cocaine maintained responding during placebo and n-acetylcysteine maintenance, but the reinforcing effects of cocaine were significantly attenuated across both maintenance conditions in participants maintained on n-acetylcysteine first compared to participants maintained on placebo first. These results collectively suggest that a reduction in the incentive salience of cocaine-related stimuli during n-acetylcysteine maintenance may be accompanied by reductions in cocaine self-administration. These results are in agreement with, and link, prior preclinical and clinical trial results suggesting that n-acetylcysteine might be useful for preventing cocaine relapse by attenuating the incentive salience of cocaine cues. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. External anal sphincter fatigue is not improved by N-acetylcysteine in an animal model. (United States)

    Healy, C F; McMorrow, C; O'Herlihy, C; O'Connell, P R; Jones, J F X


    Oxidative stress is associated with skeletal muscle fatigue. This study tests the hypotheses that N-acetylcysteine (NAC) reduces fatigue and accelerates recovery of the rat external anal sphincter (EAS). Fifteen female Wistar rats were killed humanely. The EAS was mounted as a ring preparation and electrically stimulated with 50 Hz trains of 200 ms in duration every 4 s for three and a half minutes. Three groups were analysed: a control group (n = 5), a group pretreated with NAC (10(-4) mol L(-1); n = 5) and a group pretreated with NAC (10(-3) mol L(-1); n = 5). A novel fatigue index was formulated and was compared to a conventional method of expressing fatigue. There was no significant difference at concentrations of NAC (10(-4) mol L(-1); P > 0.05). At high concentrations of NAC (10(-3) mol L(-1)) there was a significant depression in peak twitch amplitude before fatigue (P = 0.04). N-acetylcysteine in both concentrations used, did not alter fatigue or recovery of the rat EAS. There was a significant positive correlation between the two methods of expressing fatigue but the conventional method produced a higher fatigue index (22.4% on average). N-acetylcysteine does not ameliorate fatigue or accelerate recovery of the EAS and may not be a useful medical therapy for faecal incontinence.

  9. N-Acetylcysteine's Role in Sepsis and Potential Benefit in Patients With Microcirculatory Derangements. (United States)

    Chertoff, Jason


    To review the data surrounding the utility of N-acetylcysteine (NAC) in sepsis and identify areas needed for additional research. A review of articles describing the mechanisms of action and clinical use of NAC in sepsis. Despite many advances in critical care medicine, still as many as 50% of patients with septic shock die. Treatments thus far have focused on resuscitation and restoration of macrocirculatory targets in the early phases of sepsis, with less focus on microcirculatory dysfunction. N-acetylcysteine, due to its anti-inflammatory and antioxidative properties, has been readily investigated in sepsis and has yielded largely incongruous and disappointing results. In addition to its known anti-inflammatory and antioxidative roles, one underappreciated property of NAC is its ability to vasodilate the microcirculation and improve locoregional blood flow. Some investigators have sought to capitalize on this mechanism with promising results, as evidenced by microcirculatory vasodilation, improvements in regional blood flow and oxygen delivery, and reductions in lactic acidosis, organ failure, and mortality. In addition to its antioxidant and anti-inflammatory properties, N-acetylcysteine possesses vasodilatory properties that could benefit the microcirculation in sepsis. It is imperative that we investigate these properties to uncover NAC's full potential for benefit in sepsis.

  10. Effect of N-Acetylcysteine in Protecting from Simultaneous Noise and Carbon Monoxide Induced Hair Cell Loss

    Directory of Open Access Journals (Sweden)

    Akram Pourbakht


    Full Text Available Background and Aim: N-acetylcysteine, a glutathione precursor and reactive oxygen species scavenger, is reported to be effective in reducing noise-induced hearing loss. Many workers in industry are exposed simultaneously to noise and chemical pollutants such as carbon monoxide. We investigated effectiveness of N-acetylcysteine in protecting the cochlea from simultaneous noise and carbon monoxide damages.Methods: Twelve rabbits were exposed simeltaneously to 100 dB sound pressure level of broad band noise and carbon monoxide 8 hours a day for 5 days. One hour before exposure, experimental group received 325 mg/kg of N-acetylcysteine while normal saline was administered for the control group. The protective effect of N-acetylcysteine was evaluated 3 weeks after exposure by histological assessment of the hair cells.Results: Simultaneous exposure to noise and carbon monoxide resulted in a considerable damage to the outer hair cells; however, the inner hair cells and the pillar cells remained intact. Use of N-acetylcysteine in the experimental group significantly reduced the extent of outer hair cell loss.Conclusion: N-acetylcysteine attenuates simultaneous noise and carbon monoxide induced hair cell damage in rabbits.

  11. N-acetylcysteine, a glutamate modulator, in the treatment of trichotillomania: a double-blind, placebo-controlled study. (United States)

    Grant, Jon E; Odlaug, Brian L; Kim, Suck Won


    Trichotillomania is characterized by repetitive hair pulling that causes noticeable hair loss. Data on the pharmacologic treatment of trichotillomania are limited to conflicting studies of serotonergic medications. N-acetylcysteine, an amino acid, seems to restore the extracellular glutamate concentration in the nucleus accumbens and, therefore, offers promise in the reduction of compulsive behavior. To determine the efficacy and tolerability of N-acetylcysteine in adults with trichotillomania. Twelve-week, double-blind, placebo-controlled trial. Ambulatory care center. Fifty individuals with trichotillomania (45 women and 5 men; mean [SD] age, 34.3 [12.1] years). N-acetylcysteine (dosing range, 1200-2400 mg/d) or placebo was administered for 12 weeks. Patients were assessed using the Massachusetts General Hospital Hair Pulling Scale, the Clinical Global Impression scale, the Psychiatric Institute Trichotillomania Scale, and measures of depression, anxiety, and psychosocial functioning. Outcomes were examined using analysis of variance modeling analyses and linear regression in an intention-to-treat population. Patients assigned to receive N-acetylcysteine had significantly greater reductions in hair-pulling symptoms as measured using the Massachusetts General Hospital Hair Pulling Scale (P acetylcysteine use compared with 16% taking placebo (P = .003). Significant improvement was initially noted after 9 weeks of treatment. This study, the first to our knowledge that examines the efficacy of a glutamatergic agent in the treatment of trichotillomania, found that N-acetylcysteine demonstrated statistically significant reductions in trichotillomania symptoms. No adverse events occurred in the N-acetylcysteine group, and N-acetylcysteine was well tolerated. Pharmacologic modulation of the glutamate system may prove to be useful in the control of a range of compulsive behaviors. Identifier: NCT00354770.

  12. Possible involvement of the JAK/STAT signaling pathway in N-acetylcysteine-mediated antidepressant-like effects. (United States)

    Al-Samhari, Marwa M; Al-Rasheed, Nouf M; Al-Rejaie, Salim; Al-Rasheed, Nawal M; Hasan, Iman H; Mahmoud, Ayman M; Dzimiri, Nduna


    Advances in depression research have targeted inflammation and oxidative stress to develop novel types of treatment. The JAK/STAT signaling pathway plays pivotal roles in immune and inflammatory responses. The present study was designed to investigate the effects of N-acetylcysteine, a putative precursor of the antioxidant glutathione, in an animal model of depression, with an emphasis on the JAK/STAT signaling pathway. Fluoxetine, a classical antidepressant drug was also under investigation. Male Wistar rats were subjected to forced swimming test and given N-acetylcysteine and fluoxetine immediately after the pre-test session, 5 h later and 1 h before the test session of the forced swimming test. N-acetylcysteine decreased immobility time (P acetylcysteine produced significant (P acetylcysteine significantly (P acetylcysteine. Therefore, depression research may target the JAK/STAT signaling pathway to provide a novel effective therapy. © 2015 by the Society for Experimental Biology and Medicine.

  13. 7-N-Acetylcysteine-pyrrole conjugate-A potent DNA reactive metabolite of pyrrolizidine alkaloids. (United States)

    He, Xiaobo; Ma, Liang; Xia, Qingsu; Fu, Peter P


    Plants containing pyrrolizidine alkaloids (PAs) are widespread throughout the world and are the most common poisonous plants affecting livestock, wildlife, and humans. PAs require metabolic activation to form reactive dehydropyrrolizidine alkaloids (dehydro-PAs) that are capable of alkylating cellular DNA and proteins, form (±)-6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine (DHP)-DNA and DHP-protein adducts, and lead to cytotoxicity, genotoxicity, and tumorigenicity. In this study, we determined that the metabolism of riddelliine and monocrotaline by human and rat liver microsomes in the presence of N-acetylcysteine both produced 7-N-acetylcysteine-DHP (7-NAC-DHP) and DHP. Reactions of 7-NAC-DHP with 2'-deoxyguanosine (dG), 2'-deoxyadenosine (dA), and calf thymus DNA in aqueous solution followed by enzymatic hydrolysis yielded DHP-dG and/or DHP-dA adducts. These results indicate that 7-NAC-DHP is a reactive metabolite that can lead to DNA adduct formation. Copyright © 2016. Published by Elsevier B.V.

  14. 7-N-Acetylcysteine-pyrrole conjugate—A potent DNA reactive metabolite of pyrrolizidine alkaloids

    Directory of Open Access Journals (Sweden)

    Xiaobo He


    Full Text Available Plants containing pyrrolizidine alkaloids (PAs are widespread throughout the world and are the most common poisonous plants affecting livestock, wildlife, and humans. PAs require metabolic activation to form reactive dehydropyrrolizidine alkaloids (dehydro-PAs that are capable of alkylating cellular DNA and proteins, form (±-6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine (DHP-DNA and DHP-protein adducts, and lead to cytotoxicity, genotoxicity, and tumorigenicity. In this study, we determined that the metabolism of riddelliine and monocrotaline by human and rat liver microsomes in the presence of N-acetylcysteine both produced 7-N-acetylcysteine-DHP (7-NAC-DHP and DHP. Reactions of 7-NAC-DHP with 2′-deoxyguanosine (dG, 2′-deoxyadenosine (dA, and calf thymus DNA in aqueous solution followed by enzymatic hydrolysis yielded DHP-dG and/or DHP-dA adducts. These results indicate that 7-NAC-DHP is a reactive metabolite that can lead to DNA adduct formation.

  15. The effect of N-acetylcysteine on cardiac contractility to dobutamine in rats with streptozotocin-induced diabetes. (United States)

    Cheng, Xing; Xia, Zhengyuan; Leo, Joyce M; Pang, Catherine C Y


    We examined if myocardial depression at the acute phase of diabetes (3 weeks after injection of streptozotocin, 60 mg/kg i.v.) is due to activation of inducible nitric oxide synthase and production of peroxynitrite, and if treatment with N-acetylcysteine (1.2 g/day/kg for 3 weeks, antioxidant) improves cardiac function. Four groups of rats were used: control, N-acetylcysteine-treated control, diabetic and N-acetylcysteine-treated diabetic. Pentobarbital-anaesthetized diabetic rats, relative to the controls, had reduced left ventricular contractility to dobutamine (1-57 microg/min/kg). The diabetic rats also had increased myocardial levels of thiobarbituric acid reactive substances, immunostaining of inducible nitric oxide synthase and nitrotyrosine, and similar baseline 15-F2t-isoprostane. N-acetylcysteine did not affect responses in the control rats; but increased cardiac contractility to dobutamine, reduced myocardial immunostaining of inducible nitric oxide synthase and nitrotyrosine and level of 15-F2t-isoprostane, and increased cardiac contractility to dobutamine in the diabetic rats. Antioxidant supplementation in diabetes reduces oxidative stress and improves cardiac function.

  16. Efficacy of N-acetylcysteine in the treatment of nicotine dependence: a double-blind placebo-controlled pilot study

    NARCIS (Netherlands)

    Schmaal, L.; Berk, L.; Hulstijn, K.P.; Cousijn, J.; Wiers, R.W.; van den Brink, W.


    Relapse is the rule rather than the exception in smokers aiming to quit smoking. Recently, evidence has emerged that glutamate transmission plays an important role in relapse. N-acetylcysteine (NAC), a cysteine prodrug, restores glutamate homeostasis and appears to be a potential new treatment for

  17. N-acetylcysteine modifies the acute effects of isosorbide-5-mononitrate in angina pectoris patients evaluated by exercise testing

    DEFF Research Database (Denmark)

    Svendsen, Jesper Hastrup; Klarlund, K; Aldershvile, J


    Nitrates are well established in the treatment of angina pectoris and the presence of sulfhydryl groups seems to be fundamental to nitrate-induced vasodilatation. The present study was performed to elucidate if large oral doses of N-acetylcysteine (NAC, 2,400 mg X 2), a donor of sulfhydryl groups...

  18. N-Acetylcysteine Normalizes Glutamate Levels in Cocaine-Dependent Patients: A Randomized Crossover Magnetic Resonance Spectroscopy Study

    NARCIS (Netherlands)

    Schmaal, Lianne; Veltman, Dick J.; Nederveen, Aart; van den Brink, Wim; Goudriaan, Anna E.


    Treatment with N-acetylcysteine (NAC) normalizes glutamate (Glu) homeostasis and prevents relapse in drug-dependent animals. However, the effect of NAC on brain Glu levels in substance-dependent humans has not yet been investigated. Proton magnetic resonance spectroscopy (H-1 MRS) was used to

  19. Antihypertensive mechanisms of chronic captopril (CPT) or N-Acetylcysteine (NAC) treatment in L-NAME hypertensive rats

    Czech Academy of Sciences Publication Activity Database

    Dobešová, Zdenka; Zicha, Josef; Pecháňová, Olga; Kuneš, Jaroslav


    Roč. 46, č. 4 (2005), s. 912-913 ISSN 0194-911X. [Annual Meeting of the European Council for Cardiovascular Research (ECCR) /10./. 14.10.2005-16.10.2005, La Colle sur Loup] R&D Projects: GA MZd(CZ) NR7786 Keywords : antihypertensive mechanism * captopril * N-Acetylcysteine * L-NAME hypertension Subject RIV: ED - Physiology

  20. N-Acetylcysteine Normalizes Glutamate Levels in Cocaine-Dependent Patients: A Randomized Crossover Magnetic Resonance Spectroscopy Study

    NARCIS (Netherlands)

    Schmaal, L.; Veltman, D.J.; Nederveen, A.; van den Brink, W.; Goudriaan, A.E.


    Treatment with N-acetylcysteine (NAC) normalizes glutamate (Glu) homeostasis and prevents relapse in drug-dependent animals. However, the effect of NAC on brain Glu levels in substance-dependent humans has not yet been investigated. Proton magnetic resonance spectroscopy (1 H MRS) was used to

  1. [A girl with self-harm treated with N-acetylcysteine (NAC)]. (United States)

    Rus, C P

    Deliberate and recurrent self-harm could be regarded as addictive behaviour that can be treated with medication. In addiction, the dopaminergic mesolimbic reward system is activated. Pain caused by cutting stimulates the reward system through the opioid system. Glutamatergic neurotransmission follows the same pathway and plays a role in addiction as well. In this case-study a 17-year-old girl was successfully treated with N-acetylcysteine (nac) in order to reduce the frequency of self-cutting. In addition, in this case nac reduced the symptoms of attention deficit/hyperactivity disorder and depression. nac modulates the glutamatergic neurotransmission. This article provides possible explanations for the effect of nac in this case.

  2. N-acetylcysteine for neuropsychiatric symptoms in a woman with Williams syndrome. (United States)

    Pineiro, Mildred Lopez; Roberts, Antoinette M; Waxler, Jessica L; Mullett, Jennifer E; Pober, Barbara R; McDougle, Christopher J


    Williams syndrome is a relatively rare genetic disorder caused by the hemizygous microdeletion of a region in chromosome 7q11.23. Individuals with Williams syndrome typically present with a highly social, overfriendly, and empathic personality. Comorbid medical and neuropsychiatric disorders are common. Reports of effective pharmacological treatment of associated neuropsychiatric disorders are limited. The authors describe the successful treatment of interfering anger, aggression, and hair-pulling with N-acetylcysteine in a 19-year-old woman with Williams syndrome. The neuropsychiatric symptoms emerged 1 week following an upper gastrointestinal endoscopy, for which fentanyl, midazolam, and propofol were used as anesthetics. The patient's treatment course and hypothesized mechanisms underlying the clinical presentation and symptom resolution are described. © The Author(s) 2014.

  3. The protective effect of N-acetylcysteine on oxidative stress in the brain caused by the long-term intake of aspartame by rats. (United States)

    Finamor, Isabela A; Ourique, Giovana M; Pês, Tanise S; Saccol, Etiane M H; Bressan, Caroline A; Scheid, Taína; Baldisserotto, Bernardo; Llesuy, Susana F; Partata, Wânia A; Pavanato, Maria A


    Long-term intake of aspartame at the acceptable daily dose causes oxidative stress in rodent brain mainly due to the dysregulation of glutathione (GSH) homeostasis. N-Acetylcysteine provides the cysteine that is required for the production of GSH, being effective in treating disorders associated with oxidative stress. We investigated the effects of N-acetylcysteine treatment (150 mg kg(-1), i.p.) on oxidative stress biomarkers in rat brain after chronic aspartame administration by gavage (40 mg kg(-1)). N-Acetylcysteine led to a reduction in the thiobarbituric acid reactive substances, lipid hydroperoxides, and carbonyl protein levels, which were increased due to aspartame administration. N-Acetylcysteine also resulted in an elevation of superoxide dismutase, glutathione peroxidase, glutathione reductase activities, as well as non-protein thiols, and total reactive antioxidant potential levels, which were decreased after aspartame exposure. However, N-acetylcysteine was unable to reduce serum glucose levels, which were increased as a result of aspartame administration. Furthermore, catalase and glutathione S-transferase, whose activities were reduced due to aspartame treatment, remained decreased even after N-acetylcysteine exposure. In conclusion, N-acetylcysteine treatment may exert a protective effect against the oxidative damage in the brain, which was caused by the long-term consumption of the acceptable daily dose of aspartame by rats.

  4. Possible involvement of the JAK/STAT signaling pathway in N-acetylcysteine-mediated antidepressant-like effects


    Al-Samhari, Marwa M; Al-Rasheed, Nouf M; Al-Rejaie, Salim; Al-Rasheed, Nawal M; Hasan, Iman H; Mahmoud, Ayman M; Dzimiri, Nduna


    Advances in depression research have targeted inflammation and oxidative stress to develop novel types of treatment. The JAK/STAT signaling pathway plays pivotal roles in immune and inflammatory responses. The present study was designed to investigate the effects of N-acetylcysteine, a putative precursor of the antioxidant glutathione, in an animal model of depression, with an emphasis on the JAK/STAT signaling pathway. Fluoxetine, a classical antidepressant drug was also under investigation....

  5. N-Acetylcysteine as adjunctive treatment in severe malaria: A randomized double blinded placebo controlled clinical trial (United States)

    Charunwatthana, Prakaykaew; Faiz, M. Abul; Ruangveerayut, Ronnatrai; Maude, Richard; Rahman, M. Ridwanur; Roberts, L. Jackson; Moore, Kevin; Yunus, Emran Bin; Hoque, M. Gofranul; Hasan, Mahatab Uddin; Lee, Sue J.; Pukrittayakamee, Sasithon; Newton, Paul N.; White, Nicholas J.; Day, Nicholas P.J.; Dondorp, Arjen M.


    Objective Markers of oxidative stress are reported to be increased in severe malaria. It has been suggested that the antioxidant N-acetylcysteine (NAC) may be beneficial in treatment. We studied the efficacy and safety of parenteral N-acetylcysteine as an adjunct to artesunate treatment of severe falciparum malaria. Design A randomized double-blind placebo controlled trial on the use of high dose intravenous NAC as adjunctive treatment to artesunate. Setting A provincial hospital in Western Thailand and a tertiary referral hospital in Chittagong, Bangladesh. Patients One hundred and eight adult patients with severe falciparum malaria. Interventions Patients were randomized to receive N-acetylcysteine or placebo as adjunctive treatment to intravenous artesunate. Measurements and main results A total of 56 patients were treated with NAC and 52 received placebo. NAC had no significant effect on mortality, lactate clearance times (p=0.74) or coma recovery times (p=0.46). Parasite clearance time was increased from 30h (range 6h to 144h) to 36h (range 6h to 120h) (p=0.03), but this could be explained by differences in admission parasitemia. Urinary F2-isoprostane metabolites, measured as a marker of oxidative stress, were increased in severe malaria compared to patients with uncomplicated malaria and healthy volunteers. Admission red cell rigidity correlated with mortality, but did not improve with NAC. Conclusion Systemic oxidative stress is increased in severe malaria. Treatment with N-acetylcysteine had no effect on outcome in patients with severe falciparum malaria in this setting. PMID:19114891

  6. Systematic review of N-acetylcysteine in the treatment of addictions

    Directory of Open Access Journals (Sweden)

    Elson Asevedo


    Full Text Available Objective: To conduct the first systematic literature review of clinical trials of N-acetylcysteine (NAC for the treatment of substance abuse disorders and addictive behaviors. Methods: A search of the MEDLINE, Embase and PsycINFO databases was conducted. The inclusion criteria for the review were clinical trials that used NAC in the treatment of a disorder related to substance use and/or addictive behaviors, limited to texts in English, Spanish, or French. The selected studies were evaluated with respect to type of trial, sample size, diagnostic input, intervention, length of follow-up, outcome variables, and results. Results: Nine studies analyzing a total of 165 patients met the eligibility criteria and were included in qualitative analysis. These studies evaluated the role of NAC in cocaine dependence (three studies, cannabis dependence (two studies, nicotine dependence (two studies, methamphetamine addiction (one study, and pathological gambling (one study. Five of these trials were double-blind, randomized, and placebo-controlled. Conclusions: The studies analyzed suggest a potential role for NAC in the treatment of addiction, especially of cocaine and cannabis dependence. These results are concordant with the hypothesis of the involvement of glutamatergic pathways in the pathophysiology of addiction.

  7. Effects of N-acetylcysteine on semen parameters and oxidative/antioxidant status. (United States)

    Ciftci, Halil; Verit, Ayhan; Savas, Murat; Yeni, Ercan; Erel, Ozcan


    To examine whether a beneficial effect of N-acetylcysteine (NAC) on semen parameters and oxidative/antioxidant status in idiopathic male infertility exists. The production of reactive oxygen species is a normal physiologic event in various organs. However, overproduction of reactive oxygen species can be detrimental to sperm and has been associated with male infertility. Our study included 120 patients who had attended our clinic and were diagnosed with idiopathic infertility according to medical history and physical and seminal examination findings, as initial evaluations. The patients were divided randomly into 2 groups. Those in the study group (60 men) were given NAC (600 mg/d orally) for 3 months; the control group (60 men) received a placebo. The oxidative status was determined by measuring the total antioxidant capacity, total peroxide and oxidative stress index in plasma samples. The sperm parameters were evaluated after NAC treatment and were compared with those in the control group. NAC had significant improving effects on the volume, motility, and viscosity of semen. After NAC treatment, the serum total antioxidant capacity was greater and the total peroxide and oxidative stress index were lower in the NAC-treated group compared with the control group. These beneficial effects resulted from reduced reactive oxygen species in the serum and reduced viscosity of the semen. No significant differences were found in the number or morphology of the sperm between the 2 groups. We believe that NAC could improve some semen parameters and the oxidative/antioxidant status in patients with male infertility.

  8. N-acetylcysteine prevents the development of gastritis induced by Helicobacter pylori infection. (United States)

    Jang, Sungil; Bak, Eun-Jung; Cha, Jeong-Heon


    Helicobacter pylori (H. pylori) is a human gastric pathogen, causing various gastric diseases ranging from gastritis to gastric adenocarcinoma. It has been reported that combining N-acetylcysteine (NAC) with conventional antibiotic therapy increases the success rate of H. pylori eradication. We evaluated the effect of NAC itself on the growth and colonization of H. pylori, and development of gastritis, using in vitro liquid culture system and in vivo animal models. H. pylori growth was evaluated in broth culture containing NAC. The H. pylori load and histopathological scores of stomachs were measured in Mongolian gerbils infected with H. pylori strain 7.13, and fed with NAC-containing diet. In liquid culture, NAC inhibited H. pylori growth in a concentration-dependent manner. In the animal model, 3-day administration of NAC after 1 week from infection reduced the H. pylori load; 6-week administration of NAC after 1 week from infection prevented the development of gastritis and reduced H. pylori colonization. However, no reduction in the bacterial load or degree of gastritis was observed with a 6-week administration of NAC following 6-week infection period. Our results indicate that NAC may exert a beneficial effect on reduction of bacterial colonization, and prevents the development of severe inflammation, in people with initial asymptomatic or mild H. pylori infection.

  9. Clinical trials of N-acetylcysteine in psychiatry and neurology: A systematic review. (United States)

    Deepmala; Slattery, John; Kumar, Nihit; Delhey, Leanna; Berk, Michael; Dean, Olivia; Spielholz, Charles; Frye, Richard


    N-acetylcysteine (NAC) is recognized for its role in acetaminophen overdose and as a mucolytic. Over the past decade, there has been growing evidence for the use of NAC in treating psychiatric and neurological disorders, considering its role in attenuating pathophysiological processes associated with these disorders, including oxidative stress, apoptosis, mitochondrial dysfunction, neuroinflammation and glutamate and dopamine dysregulation. In this systematic review we find favorable evidence for the use of NAC in several psychiatric and neurological disorders, particularly autism, Alzheimer's disease, cocaine and cannabis addiction, bipolar disorder, depression, trichotillomania, nail biting, skin picking, obsessive-compulsive disorder, schizophrenia, drug-induced neuropathy and progressive myoclonic epilepsy. Disorders such as anxiety, attention deficit hyperactivity disorder and mild traumatic brain injury have preliminary evidence and require larger confirmatory studies while current evidence does not support the use of NAC in gambling, methamphetamine and nicotine addictions and amyotrophic lateral sclerosis. Overall, NAC treatment appears to be safe and tolerable. Further well designed, larger controlled trials are needed for specific psychiatric and neurological disorders where the evidence is favorable. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. N-acetylcysteine prevents stress-induced anxiety behavior in zebrafish. (United States)

    Mocelin, Ricieri; Herrmann, Ana P; Marcon, Matheus; Rambo, Cassiano L; Rohden, Aline; Bevilaqua, Fernanda; de Abreu, Murilo Sander; Zanatta, Leila; Elisabetsky, Elaine; Barcellos, Leonardo J G; Lara, Diogo R; Piato, Angelo L


    Despite the recent advances in understanding the pathophysiology of anxiety disorders, the pharmacological treatments currently available are limited in efficacy and induce serious side effects. A possible strategy to achieve clinical benefits is drug repurposing, i.e., discovery of novel applications for old drugs, bringing new treatment options to the market and to the patients who need them. N-acetylcysteine (NAC), a commonly used mucolytic and paracetamol antidote, has emerged as a promising molecule for the treatment of several neuropsychiatric disorders. The mechanism of action of this drug is complex, and involves modulation of antioxidant, inflammatory, neurotrophic and glutamate pathways. Here we evaluated the effects of NAC on behavioral parameters relevant to anxiety in zebrafish. NAC did not alter behavioral parameters in the novel tank test, prevented the anxiety-like behaviors induced by an acute stressor (net chasing), and increased the time zebrafish spent in the lit side in the light/dark test. These data may indicate that NAC presents an anti-stress effect, with the potential to prevent stress-induced psychiatric disorders such as anxiety and depression. The considerable homology between mammalian and zebrafish genomes invests the current data with translational validity for the further clinical trials needed to substantiate the use of NAC in anxiety disorders. Copyright © 2015 Elsevier Inc. All rights reserved.

  11. Compatibility and osmolality of inhaled N-acetylcysteine nebulizing solution with fenoterol and ipratropium. (United States)

    Lee, Tzung-Yi; Chen, Chi-Ming; Lee, Chun-Nin; Chiang, Yi-Chun; Chen, Hsiang-Yin


    The compatibility, pH, and osmolality of N-acetylcysteine (NAC) nebulizing solution in the presence of ipratropium bromide or fenoterol hydrobromide were studied. Portions (400 microL) of each mixture were sampled immediately upon mixing and one, two, three, four, five, six, and seven hours after mixing and assayed by high-performance liquid chromatography. Osmolality was measured by sampling 100 microL from the filling cup at a five-minute interval during nebulization and by the freezing-point-depression method. Adding NAC solution to fenoterol solution raised the pH from 3.20 to 7.90 and the osmolality to a mean +/- S.D. of 1400.67 +/- 4.51 mOsm/kg. Fenoterol concentrations decreased to 93.71% and NAC concentrations to 92.54% of initial concentrations after seven hours. Mixing ipratropium with NAC solution raised the pH from 3.74 to 7.95 and the osmolality to a mean +/- S.D. of 1413 +/- 11.79 mOsm/kg. The initial ipratropium concentration declined 7.39% and 10.91% one and two hours after mixing with NAC solution, respectively. NAC and ipratropium were stable in nebulizing solution within one hour of mixing. NAC and fenoterol were compatible for at least seven hours.

  12. N-acetylcysteine in the treatment of psychiatric disorders: current status and future prospects. (United States)

    Minarini, Alessandro; Ferrari, Silvia; Galletti, Martina; Giambalvo, Nina; Perrone, Daniela; Rioli, Giulia; Galeazzi, Gian Maria


    N-acetylcysteine (NAC) is widely known for its role as a mucolytic and as an antidote to paracetamol overdose. There is increasing interest in the use of NAC in the treatment of several psychiatric disorders. The rationale for the administration of NAC in psychiatric conditions is based on its role as a precursor to the antioxidant glutathione, and its action as a modulating agent of glutamatergic, dopaminergic, neurotropic and inflammatory pathways. Areas covered: This study reviews the available data regarding the use of NAC in different psychiatric disorders including substance use disorders, autism, obsessive-compulsive spectrum disorders, schizophrenia, depression, bipolar disorder. Promising results were found in trials testing the use of NAC, mainly as an add-on treatment, in cannabis use disorder in young people, depression in bipolar disorder, negative symptoms in schizophrenia, and excoriation (skin-picking) disorder. Despite initial optimism, recent findings regarding NAC efficacy in autism have been disappointing. Expert opinion: These preliminary positive results require further confirmation in larger samples and with longer follow-ups. Given its high tolerability and wide availability, NAC represents an important target to investigate in the field of new adjunctive treatments for psychiatric conditions.

  13. Current evidence for the use of N-acetylcysteine following liver resection. (United States)

    Kemp, Richard; Mole, Jonathan; Gomez, Dhanny


    N-acetylcysteine (NAC) has many uses in medicine; notable in the management of paracetamol toxicity, acute liver failure and liver surgery. The aim of this review was to critically appraise the published literature for the routine use of NAC in liver resection surgery. An electronic search was performed of EBSCOhost (Medline and CINAHL database), PubMed and the Cochrane Library for the period 1990-2016. MeSH headings: 'acetyl-cysteine', 'liver resection' and 'hepatectomy' were used to identify all relevant articles published in English. Following the search criteria used, three articles were included. Two of these studies were randomized controlled trials. All the studies collated data on morbidity and mortality. All three studies did not show a significant difference in overall complications rates in patients that underwent hepatic resection that had NAC infusion compared with patients that did not. In one study, NAC administration was associated with a higher frequency of grade A post-hepatectomy liver failure. In another study, a significantly higher incidence of delirium was observed in the NAC group, which led to the trial to be terminated early. The current published data do not support the routine use of NAC following liver resection. © 2017 Royal Australasian College of Surgeons.

  14. N-Acetylcysteine as an antioxidant and disulphide breaking agent: the reasons why. (United States)

    Aldini, Giancarlo; Altomare, Alessandra; Baron, Giovanna; Vistoli, Giulio; Carini, Marina; Borsani, Luisa; Sergio, Francesco


    The main molecular mechanisms explaining the well-established antioxidant and reducing activity of N-acetylcysteine (NAC), the N-acetyl derivative of the natural amino acid l-cysteine, are summarised and critically reviewed. The antioxidant effect is due to the ability of NAC to act as a reduced glutathione (GSH) precursor; GSH is a well-known direct antioxidant and a substrate of several antioxidant enzymes. Moreover, in some conditions where a significant depletion of endogenous Cys and GSH occurs, NAC can act as a direct antioxidant for some oxidant species such as NO 2 and HOX. The antioxidant activity of NAC could also be due to its effect in breaking thiolated proteins, thus releasing free thiols as well as reduced proteins, which in some cases, such as for mercaptoalbumin, have important direct antioxidant activity. As well as being involved in the antioxidant mechanism, the disulphide breaking activity of NAC also explains its mucolytic activity which is due to its effect in reducing heavily cross-linked mucus glycoproteins. Chemical features explaining the efficient disulphide breaking activity of NAC are also explained.

  15. Thiol Redox Transitions in Cell Signaling: a Lesson from N-Acetylcysteine

    Directory of Open Access Journals (Sweden)

    Tiziana Parasassi


    Full Text Available The functional status of cells is under the control of external stimuli affecting the function of critical proteins and eventually gene expression. Signal sensing and transduction by messengers to specific effectors operate by post-translational modification of proteins, among which thiol redox switches play a fundamental role that is just beginning to be understood. The maintenance of the redox status is, indeed, crucial for cellular homeostasis and its dysregulation towards a more oxidized intracellular environment is associated with aberrant proliferation, ultimately related to diseases such as cancer, cardiovascular disease, and diabetes. Redox transitions occur in sensitive cysteine residues of regulatory proteins relevant to signaling, their evolution to metastable disulfides accounting for the functional redox switch. N-acetylcysteine (NAC is a thiol-containing compound that is able to interfere with redox transitions of thiols and, thus, in principle, able to modulate redox signaling. We here review the redox chemistry of NAC, then screen possible mechanisms to explain the effects observed in NAC-treated normal and cancer cells; such effects involve a modification of global gene expression, thus of functions and morphology, with a leitmotif of a switch from proliferation to terminal differentiation. The regulation of thiol redox transitions in cell signaling is, therefore, proposed as a new tool, holding promise not only for a deeper explanation of mechanisms, but indeed for innovative pharmacological interventions.

  16. N-acetylcysteine reduces the renal oxidative stress and apoptosis induced by hemorrhagic shock. (United States)

    Moreira, Miriam Aparecida; Irigoyen, Maria Claudia; Saad, Karen Ruggeri; Saad, Paulo Fernandes; Koike, Marcia Kiyomi; Montero, Edna Frasson de Souza; Martins, José Luiz


    Renal ischemia/reperfusion injury induced by hemorrhagic shock (HS) and subsequent fluid resuscitation is a common cause of acute renal failure. The objective of this study was to evaluate the effect of combining N-acetylcysteine (NAC) with fluid resuscitation on renal injury in rats that underwent HS. Two groups of male Wistar rats were induced to controlled HS at 35 mm Hg mean arterial pressure for 60 min. After this period, the HS and fluid resuscitation (HS/R) group was resuscitated with lactate containing 50% of the blood that was withdrawn. The HS/R + NAC group was resuscitated with Ringer's lactate combined with 150 mg/kg of NAC and blood. The sham group animals were catheterized but were not subjected to shock. All animals were kept under anesthesia and euthanized after 120 min of fluid resuscitation or observation. Animals treated with NAC presented attenuation of histologic lesions, reduced oxidative stress, and apoptosis markers when compared with animals from the HS/R group. The serum creatinine was similar in all the groups. NAC is a promising drug for combining with fluid resuscitation to attenuate the kidney injury associated with HS. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. Effect of N-acetylcysteine treatment on oxidative stress and inflammation after severe burn. (United States)

    Csontos, C; Rezman, B; Foldi, V; Bogar, L; Drenkovics, L; Röth, E; Weber, G; Lantos, J


    Oxidative stress and inflammation generate edema in burns. The aim of our study was to assess effect of N-acetylcysteine (NAC) on oxidative stress, inflammation, fluid requirement, multiple organ dysfunction (MOD) score and vasoactive drug requirement. In this study 15 patients were on standard therapy, whereas for other 15 patients NAC was supplemented. Blood samples were taken on admission and on the next five consecutive mornings. Levels of malondialdehyde, protein sulfhydril (PSH) groups, reduced gluthation (GSH), activity of myeloperoxidase, catalase and superoxide dismutase enzymes and induced free radical generating capacity were measured as well as concentrations of TNF-α, IL-6, IL-8, and IL-10. MOD score, use of vasopressor agents and fluid utilisation were recorded daily. NAC treatment increased GSH level on days 4-5 (ptreatment is associated with a diminished oxidative stress reflected in preserved antioxidant levels, lower inflammation mirrored in lower interleukin levels and less vasopressor requirement. Copyright © 2011 Elsevier Ltd and ISBI. All rights reserved.

  18. The effect of N-acetylcysteine or bupropion on methamphetamine self-administration and methamphetamine-triggered reinstatement of female rats. (United States)

    Charntikov, Sergios; Pittenger, Steven T; Pudiak, Cindy M; Bevins, Rick A


    N-acetylcysteine and bupropion are two promising candidate medications for treatment of substance use disorder. The effects of N-acetylcysteine or bupropion on methamphetamine self-administration of female rats are not well understood. To fill this gap, this study assessed the effects of N-acetylcysteine (0, 30, 60, or 120 mg/kg) and bupropion (0, 10, 30, and 60 mg/kg) on methamphetamine self-administration of female rats across the natural estrous cycle. Following a completed dose-response curve, responding for methamphetamine self-administration was extinguished and the effects of N-acetylcysteine or bupropion on methamphetamine-triggered reinstatement was evaluated in separate experiments. N-acetylcysteine did not decrease responding maintained by methamphetamine or methamphetamine-triggered reinstatement. Bupropion significantly decreased methamphetamine self-administration and methamphetamine-triggered reinstatement in female rats with highest dose (60 mg/kg) also significantly decreasing general chamber activity. In a companion experiment, testing the effect of bupropion on responding maintained by sucrose, we confirmed non-specificity of bupropion's effects as bupropion also decreased responding for sucrose. Considered together, our findings suggest that while N-acetylcysteine has considerable promise for treatment of cocaine dependence it may not generalize to other stimulants like methamphetamine. Furthermore, although bupropion has been shown to effectively decrease methamphetamine self-administration, and presently methamphetamine-triggered reinstatement, its locomotor and reward suppressing effects warrant further investigation including both sexes. Copyright © 2018 Elsevier Ltd. All rights reserved.

  19. A Double-Blind, Randomized, Controlled Pilot Trial of N-Acetylcysteine in Veterans With Posttraumatic Stress Disorder and Substance Use Disorders. (United States)

    Back, Sudie E; McCauley, Jenna L; Korte, Kristina J; Gros, Daniel F; Leavitt, Virginia; Gray, Kevin M; Hamner, Mark B; DeSantis, Stacia M; Malcolm, Robert; Brady, Kathleen T; Kalivas, Peter W


    The antioxidant N-acetylcysteine is being increasingly investigated as a therapeutic agent in the treatment of substance use disorders (SUDs). This study explored the efficacy of N-acetylcysteine in the treatment of posttraumatic stress disorder (PTSD), which frequently co-occurs with SUD and shares impaired prefrontal cortex regulation of basal ganglia circuitry, in particular at glutamate synapses in the nucleus accumbens. Veterans with PTSD and SUD per DSM-IV criteria (N = 35) were randomly assigned to receive a double-blind, 8-week course of N-acetylcysteine (2,400 mg/d) or placebo plus cognitive-behavioral therapy for SUD (between March 2013 and April 2014). Primary outcome measures included PTSD symptoms (Clinician-Administered PTSD Scale, PTSD Checklist-Military) and craving (Visual Analog Scale). Substance use and depression were also assessed. Participants treated with N-acetylcysteine compared to placebo evidenced significant improvements in PTSD symptoms, craving, and depression (β values acetylcysteine was well tolerated, and retention was high. This is the first randomized controlled trial to investigate N-acetylcysteine as a pharmacologic treatment for PTSD and SUD. Although preliminary, the findings provide initial support for the use of N-acetylcysteine in combination with psychotherapy among individuals with co-occurring PTSD and SUD. identifier: NCT02499029. © Copyright 2016 Physicians Postgraduate Press, Inc.

  20. N-acetylcysteine Ameliorates Prostatitis via miR-141 Regulating Keap1/Nrf2 Signaling. (United States)

    Wang, Liang-Liang; Huang, Yu-Hua; Yan, Chun-Yin; Wei, Xue-Dong; Hou, Jian-Quan; Pu, Jin-Xian; Lv, Jin-Xing


    Chronic prostatitis was the most common type of prostatitis and oxidative stress was reported to be highly elevated in prostatitis patients. In this study, we determined the effect of N-acetylcysteine (NAC) on prostatitis and the molecular mechanism involved in it. Male Sprague-Dawley rats were divided into three groups: control group (group A, n = 20), carrageenan-induced chronic nonbacterial prostatitis (CNP) model group (group B, n = 20), and carrageenan-induced CNP model group with NAC injection (group C, n = 20). Eye score, locomotion score, inflammatory cell count, cyclooxygenase 2 (COX2) expression, and Evans blue were compared in these three groups. The expression of miR-141 was determined by quantitative real-time PCR (qRT-PCR). Moreover, protein expressions of Kelch-like ECH-associated protein-1 (Keap1) and nuclear factor erythroid-2 related factor 2 (Nrf2) and its target genes were examined by Western blot. Luciferase reporter assay was performed in RWPE-1 cells transfected miR-141 mimic or inhibitor and the plasmid carrying 3'-UTR of Keap1. The value of eye score, locomotion score, inflammatory cell count, and Evans blue were significantly decreased in group C, as well as the expression of COX2, when comparing to that of group B. These results indicated that NAC relieved the carrageenan-induced CNP. Further, we found that NAC increased the expression of miR-141 and activated the Keap1/Nrf2 signaling. Luciferase reporter assay revealed that miR-141 mimic could suppress the activity of Keap1 and stimulate the downstream target genes of Nrf2. In addition, miR-141 inhibitor could reduce the effect of NAC on prostatitis. NAC ameliorates the carrageenan-induced prostatitis and prostate inflammation pain through miR-141 regulating Keap1/Nrf2 signaling.

  1. N-acetylcysteine stimulates protein synthesis in enterocytes independently of glutathione synthesis. (United States)

    Yi, Dan; Hou, Yongqing; Wang, Lei; Long, Minhui; Hu, Shengdi; Mei, Huimin; Yan, Liqiong; Hu, Chien-An Andy; Wu, Guoyao


    Dietary supplementation with N-acetylcysteine (NAC) has been reported to improve intestinal health and treat gastrointestinal diseases. However, the underlying mechanisms are not fully understood. According to previous reports, NAC was thought to exert its effect through glutathione synthesis. This study tested the hypothesis that NAC enhances enterocyte growth and protein synthesis independently of cellular glutathione synthesis. Intestinal porcine epithelial cells were cultured for 3 days in Dulbecco's modified Eagle medium containing 0 or 100 μM NAC. To determine a possible role for GSH (the reduced form of glutathione) in mediating the effect of NAC on cell growth and protein synthesis, additional experiments were conducted using culture medium containing 100 μM GSH, 100 μM GSH ethyl ester (GSHee), diethylmaleate (a GSH-depletion agent; 10 μM), or a GSH-synthesis inhibitor (buthionine sulfoximine, BSO; 20 μM). NAC increased cell proliferation, GSH concentration, and protein synthesis, while inhibiting proteolysis. GSHee enhanced cell proliferation and GSH concentration without affecting protein synthesis but inhibited proteolysis. Conversely, BSO or diethylmaleate reduced cell proliferation and GSH concentration without affecting protein synthesis, while promoting protein degradation. At the signaling level, NAC augmented the protein abundance of total mTOR, phosphorylated mTOR, and phosphorylated 70S6 kinase as well as mRNA levels for mTOR and p70S6 kinase in IPEC-1 cells. Collectively, these results indicate that NAC upregulates expression of mTOR signaling proteins to stimulate protein synthesis in enterocytes independently of GSH generation. Our findings provide a hitherto unrecognized biochemical mechanism for beneficial effects of NAC in intestinal cells.

  2. N-acetylcysteine modulates glutamatergic dysfunction and depressive behavior in Huntington's disease. (United States)

    Wright, Dean J; Gray, Laura J; Finkelstein, David I; Crouch, Peter J; Pow, David; Pang, Terence Y; Li, Shanshan; Smith, Zoe M; Francis, Paul S; Renoir, Thibault; Hannan, Anthony J


    Glutamatergic dysfunction has been implicated in the pathogenesis of depressive disorders and Huntington's disease (HD), in which depression is the most common psychiatric symptom. Synaptic glutamate homeostasis is regulated by cystine-dependent glutamate transporters, including GLT-1 and system x c - In HD, the enzyme regulating cysteine (and subsequently cystine) production, cystathionine-γ-lygase, has recently been shown to be lowered. The aim of the present study was to establish whether cysteine supplementation, using N-acetylcysteine (NAC) could ameliorate glutamate pathology through the cystine-dependent transporters, system x c - and GLT-1. We demonstrate that the R6/1 transgenic mouse model of HD has lower basal levels of cystine, and showed depressive-like behaviors in the forced-swim test. Administration of NAC reversed these behaviors. This effect was blocked by co-administration of the system x c - and GLT-1 inhibitors CPG and DHK, showing that glutamate transporter activity was required for the antidepressant effects of NAC. NAC was also able to specifically increase glutamate in HD mice, in a glutamate transporter-dependent manner. These in vivo changes reflect changes in glutamate transporter protein in HD mice and human HD post-mortem tissue. Furthermore, NAC was able to rescue changes in key glutamate receptor proteins related to excitotoxicity in HD, including NMDAR2B. Thus, we have shown that baseline reductions in cysteine underlie glutamatergic dysfunction and depressive-like behavior in HD and these changes can be rescued by treatment with NAC. These findings have implications for the development of new therapeutic approaches for depressive disorders. © The Author 2016. Published by Oxford University Press. All rights reserved. For permissions, please e-mail:

  3. N-acetylcysteine for therapy-resistant tobacco use disorder: a pilot study. (United States)

    Prado, Eduardo; Maes, Michael; Piccoli, Luiz Gustavo; Baracat, Marcela; Barbosa, Décio Sabattini; Franco, Olavo; Dodd, Seetal; Berk, Michael; Vargas Nunes, Sandra Odebrecht


    N-Acetylcysteine (NAC) may have efficacy in treating tobacco use disorder (TUD) by reducing craving and smoking reward. This study examines whether treatment with NAC may have a clinical efficacy in the treatment of TUD. A 12-week double blind randomized controlled trial was conducted to compare the clinical efficacy of NAC 3 g/day versus placebo. We recruited 34 outpatients with therapy resistant TUD concurrently treated with smoking-focused group behavioral therapy. Participants had assessments of daily cigarette use (primary outcome), exhaled carbon monoxide (CO(EXH)) (secondary outcome), and quit rates as defined by CO(EXH) Depression was measured with the Hamilton Depression Rating Scale (HDRS). Data were analyzed using conventional and modified intention-to-treat endpoint analyses. NAC treatment significantly reduced the daily number of cigarettes used (Δ mean ± SD = -10.9 ± 7.9 in the NAC-treated versus -3.2 ± 6.1 in the placebo group) and CO(EXH) (Δ mean ± SD = -10.4 ± 8.6 ppm in the NAC-treated versus -1.5 ± 4.5 ppm in the placebo group); 47.1% of those treated with NAC versus 21.4% of placebo-treated patients were able to quit smoking as defined by CO(EXH) < 6 ppm. NAC treatment significantly reduced the HDRS score in patients with tobacco use disorder. These data show that treatment with NAC may have a clinical efficacy in TUD. NAC combined with appropriate psychotherapy appears to be an efficient treatment option for TUD.

  4. The role of depressive symptoms in treatment of adolescent cannabis use disorder with N-Acetylcysteine. (United States)

    Tomko, Rachel L; Gilmore, Amanda K; Gray, Kevin M


    Relative to adults, adolescents are at greater risk of developing a cannabis use disorder (CUD) and risk may be exacerbated by co-occurring depressive symptoms. N-Acetylcysteine (NAC), an over-the-counter antioxidant, is thought to normalize glutamate transmission. Oxidative stress and glutamate transmission are disrupted in both depression and CUD. Thus, NAC may be particularly effective at promoting cannabis abstinence among adolescents with elevated depressive symptoms. Secondary analyses were conducted using a sub-sample of adolescents with CUD (N = 74) who participated in an 8-week randomized placebo-controlled clinical trial examining the efficacy of NAC for cannabis cessation. It was hypothesized that NAC would reduce severity of depressive symptoms, and that decreases depressive symptom severity would mediate decreases in positive weekly urine cannabinoid tests (11-nor-9-carboxy-Δ9-tetrahydrocannabinol). Additionally, it was expected that adolescents with greater severity of baseline depressive symptoms would be more likely to become abstinent when assigned NAC relative to placebo. Results from linear mixed models and generalized estimating equations did not suggest that NAC reduced severity of depressive symptoms, and the hypothesis that NAC's effect on cannabis cessation would be mediated by reduced depressive symptoms was not supported. However, an interaction between treatment condition and baseline severity of depressive symptoms as a predictor of weekly urine cannabinoid tests was significant, suggesting that NAC was more effective at promoting abstinence among adolescents with heightened baseline depressive symptoms. These secondary findings, though preliminary, suggest a need for further examination of the role of depressive symptoms in treatment of adolescent CUD with NAC. Copyright © 2018. Published by Elsevier Ltd.

  5. Acute chloroform ingestion successfully treated with intravenously administered N-acetylcysteine. (United States)

    Dell'Aglio, Damon M; Sutter, Mark E; Schwartz, Michael D; Koch, David D; Algren, D A; Morgan, Brent W


    Chloroform, a halogenated hydrocarbon, causes central nervous system depression, cardiac arrhythmias, and hepatotoxicity. We describe a case of chloroform ingestion with a confirmatory serum level and resultant hepatotoxicity successfully treated with intravenously administered N-acetylcysteine (NAC). A 19-year-old man attempting suicide ingested approximately 75 mL of chloroform. He was unresponsive and intubated upon arrival. Intravenously administered NAC was started after initial stabilization was complete. His vital signs were normal. Admission laboratory values revealed normal serum electrolytes, AST, ALT, PT, BUN, creatinine, and bilirubin. Serum ethanol level was 15 mg/dL, and aspirin and acetaminophen were undetectable. The patient was extubated but developed liver function abnormalities with a peak AST of 224 IU/L, ALT of 583 IU/L, and bilirubin level reaching 16.3 mg/dL. NAC was continued through hospital day 6. Serum chloroform level obtained on admission was 91 μg/mL. The patient was discharged to psychiatry without known sequelae and normal liver function tests. The average serum chloroform level in fatal cases of inhalational chloroform poisoning was 64 μg/mL, significantly lower than our patient. The toxicity is believed to be similar in both inhalation and ingestion routes of exposure, with mortality predominantly resulting from anoxia secondary to central nervous system depression. Hepatocellular toxicity is thought to result from free radical-induced oxidative damage. Previous reports describe survival after treatment with orally administered NAC, we report the first use of intravenously administered NAC for chloroform ingestion. Acute oral ingestion of chloroform is extremely rare. Our case illustrates that with appropriate supportive care, patients can recover from chloroform ingestion, and intravenously administered NAC may be of benefit in such cases.

  6. N-acetylcysteine amide (AD4) reduces cocaine-induced reinstatement. (United States)

    Jastrzębska, Joanna; Frankowska, Malgorzata; Filip, Malgorzata; Atlas, Daphne


    Chronic exposure to drugs of abuse changes glutamatergic transmission in human addicts and animal models. N-acetylcysteine (NAC) is a cysteine prodrug that indirectly activates cysteine-glutamate antiporters. In the extrasynaptic space, NAC restores basal glutamate levels during drug abstinence and normalizes increased glutamatergic tone in rats during reinstatement to drugs of abuse. In initial clinical trials, repeated NAC administration seems to be promising for reduced craving in cocaine addicts. In this study, NAC-amide, called AD4 or NACA, was examined in intravenous cocaine self-administration and extinction/reinstatement procedures in rats. We investigated the behavioral effects of AD4 in the olfactory bulbectomized (OBX) rats, considered an animal model of depression. Finally, we tested rats injected with AD4 or NAC during 10-daily extinction training sessions to examine subsequent cocaine seeking. AD4 (25-75 mg kg(-1)) given acutely did not alter the rewarding effects of cocaine in OBX rats and sham-operated controls. However, at 6.25-50 mg kg(-1), AD4 decreased dose-dependently cocaine seeking and relapse triggered by cocaine priming or drug-associated conditioned cues in both phenotypes. Furthermore, repeated treatment with AD4 (25 mg kg(-1)) or NAC (100 mg kg(-1)) during daily extinction trials reduced reinstatement of drug-seeking behavior in sham-operated controls. In the OBX rats only, AD4 effectively blocked cocaine-seeking behavior. Our results demonstrate that AD4 is effective at blocking cocaine-seeking behavior, highlighting its potential clinical use toward cocaine use disorder.

  7. Topical N-acetylcysteine reduces interleukin-1-alpha in tear fluid after laser subepithelial keratectomy. (United States)

    Urgancioglu, Berrak; Bilgihan, Kamil; Engin, Doruk; Cirak, Meltem Yalinay; Hondur, Ahmet; Hasanreisoglu, Berati


    To evaluate the effect of topical N-acetylcysteine (NAC) on interleukin 1-alpha (IL-1alpha) levels in tear fluid after myopic laser subepithelial keratectomy (LASEK) and its possible role in modulating corneal wound healing. Twenty-six eyes of 13 patients who underwent myopic LASEK were divided into 2 groups. Group 1 (n=10 eyes) was used as a control group. All patients received topical lomefloxacin and dexamethasone postoperatively. Additionally, patients in Group 2 received topical NAC for 1 month postoperatively. Tear fluid samples were collected with microcapillary tubes preoperatively, on the first and on the fifth postoperative day, and the release of IL-1alpha in tear fluid was calculated. Haze grading and confocal microscopic examination were performed at 1 and 3 months postoperatively. The mean IL-1-alpha release values were 0.285-/+0.159 pg/min in Group 1 and 0.235-/+0.142 pg/min in Group 2 preoperatively. In Group 1, the values were 0.243-/+0.155 pg/min on day 1 and 0.164-/+0.125 pg/min on day 5. In Group 2, the mean IL-1alpha release values were 0.220-/+0.200 pg/min on day 1 and 0.080-/+0.079 pg/min on day 5. The difference between the groups was significant only for day 5 (p0.05). NAC seems to have an additive effect to steroids in suppressing IL-1alpha levels in tear fluid and may be clinically advantageous in modulating corneal wound healing during the early postoperative period after LASEK.

  8. l-N-acetylcysteine protects outer hair cells against TNFα initiated ototoxicity in vitro. (United States)

    Tillinger, Joshua A; Gupta, Chhavi; Ila, Kadri; Ahmed, Jamal; Mittal, Jeenu; Van De Water, Thomas R; Eshraghi, Adrien A


    The present study is aimed at determining the efficacy and exploring the mechanisms by which l-N-acetylcysteine (l-NAC) provides protection against tumor necrosis factor-alpha (TNFα)-induced oxidative stress damage and hair cell loss in 3-day-old rat organ of Corti (OC) explants. Previous work has demonstrated a high level of oxidative stress in TNFα-challenged OC explants. TNFα can potentially play a significant role in hair cell loss following an insult to the inner ear. l-NAC has shown to provide effective protection against noise-induced hearing loss in laboratory animals but mechanisms of this otoprotective effect are not well-defined. Rat OC explants were exposed to either: (1) saline control (N = 12); (2) TNFα (2 μg/ml, N = 12); (3) TNFα+l-NAC (5 mM, N = 12); (4) TNFα+l-NAC (10 mM, N = 12); or (5) l-NAC (10 mM, N = 12). Outer hair cell (OHC) density, levels of reactive oxygen species (ROS), lipid peroxidation of cell membranes, gluthathione activity, and mitochondrial viability were assayed. l-NAC (5 and 10 mM) provided protection for OHCs from ototoxic level of TNFα in OC explants. Groups treated with TNFα+l-NAC (5 mM) showed a highly significant reduction of both ROS (p l-NAC (5 mM) treated explants (p l-NAC is a promising treatment for protecting auditory HCs from TNFα-induced oxidative stress and subsequent loss via programmed cell death.

  9. Attenuation of phosphamidon-induced oxidative stress and immune dysfunction in rats treated with N-acetylcysteine

    Directory of Open Access Journals (Sweden)

    S.G. Suke


    Full Text Available The effect of N-acetylcysteine, a thiolic antioxidant, on attenuation of phosphamidon-induced oxidative stress and immune dysfunction was evaluated in adult male Wistar rats weighing 200-250 g. Rats were divided into four groups, 8 animals/group, and treated with phosphamidon, N-acetylcysteine or the combination of both for 28 days. Oral administration of phosphamidon (1.74 mg/kg, an organophosphate insecticide, increased serum malondialdehyde (3.83 ± 0.18 vs 2.91 ± 0.24 nmol/mL; P < 0.05 and decreased erythrocyte superoxide dismutase (567.8 ± 24.36 vs 749.16 ± 102.61 U/gHb; P < 0.05, catalase activity (1.86 ± 0.18 vs 2.43 ± 0.08 U/gHb; P < 0.05 and whole blood glutathione levels (1.25 ± 0.21 vs 2.28 ± 0.08 mg/gHb; P < 0.05 showing phosphamidon-induced oxidative stress. Phosphamidon exposure markedly suppressed humoral immune response as assessed by antibody titer to ovalbumin (4.71 ± 0.51 vs 8.00 ± 0.12 -log2; P < 0.05, and cell-mediated immune response as assessed by leukocyte migration inhibition (25.24 ± 1.04 vs 70.8 ± 1.09%; P < 0.05 and macrophage migration inhibition (20.38 ± 0.99 vs 67.16 ± 5.30%; P < 0.05 response. Phosphamidon exposure decreased IFN-у levels (40.7 ± 3.21 vs 55.84 ± 3.02 pg/mL; P < 0.05 suggesting a profound effect of phosphamidon on cell-mediated immune response. A phosphamidon-induced increase in TNF-α level (64.19 ± 6.0 vs 23.16 ± 4.0 pg/mL; P < 0.05 suggests a contributory role of immunocytes in oxidative stress. Co-administration of N-acetylcysteine (3.5 mmol/kg, orally with phosphamidon attenuated the adverse effects of phosphamidon. These findings suggest that oral N-acetylcysteine treatment exerts protective effect and attenuates free radical injury and immune dysfunction caused by subchronic phosphamidon exposure.

  10. The influence of single application of paracetamol and/or N-acetylcysteine on rats in subchronic exposition to trichloroethylene vapours. II. Effect on hepatic glutathione level

    Directory of Open Access Journals (Sweden)

    Danuta Plewka


    Full Text Available Background: Feature of modern existing hazards both environmental and occupational is cumulative exposure often leading to unexpected response of the organism resulting, among other things, in interactions with cytochrome P450 system involved in biotransformation of trichloroethylene and paracetamol. Hepatotoxity of paracetamol is closely connected with hepatic glutathione level. „In therapy of acute paracetamol poisoning application of N-acetylcysteine as a factor, which protects GSH level in cells, is recommended.” Materials and method: Tests were performed on rats which were treated with trichloroethylene, paracetamol and/or N-acetylcysteine. In rat liver total level of glutathione was determined i.e. reduced and oxidized form. Results: Paracetamol just after completion of the exposure affected the glutathione level. Trichloroethylene throughout the period of observation stimulated growth of glutathione level in liver. N-acetylcysteine didn’t have any influence on the level of investigated tripeptyde. Conclusions: N-acetylcysteine removes negative effect of paracetamol especially when it’s applied with 2-hour delay. After exposure for trichloethylene immediate application of N-acetylcysteine caused noticeable lowering of glutathione level. Cumulative exposure for three xenobiotics had positive influence for glutathione level in rat liver.

  11. N-Acetylcysteine protects against trichloroethene-mediated autoimmunity by attenuating oxidative stress

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    Wang, Gangduo; Wang, Jianling; Ma, Huaxian; Ansari, G.A.S.; Khan, M. Firoze, E-mail:


    Exposure to trichloroethene (TCE), a ubiquitous environmental contaminant, is known to induce autoimmunity both in humans and animal models. However, mechanisms underlying TCE-mediated autoimmunity remain largely unknown. Previous studies from our laboratory in MRL +/+ mice suggest that oxidative stress may contribute to TCE-induced autoimmune response. The current study was undertaken to further assess the role of oxidative stress in TCE-induced autoimmunity by supplementing with an antioxidant N-acetylcysteine (NAC). Groups of female MRL +/+ mice were given TCE, NAC or TCE + NAC for 6 weeks (TCE, 10 mmol/kg, i.p., every 4th day; NAC, 250 mg/kg/day through drinking water). TCE exposure led to significant increases in serum levels of anti-nuclear, anti-dsDNA and anti-Sm antibodies. TCE exposure also led to significant induction of anti-malondiadelhyde (MDA)- and anti-hydroxynonenal (HNE)-protein adduct antibodies which were associated with increased ANA in the sera along with increased MDA-/HNE-protein adducts in the livers and kidneys, and increases in protein oxidation (carbonylation) in the sera, livers and kidneys, suggesting an overall increase in oxidative stress. Moreover, TCE exposure also resulted in increased release of IL-17 from splenocytes and increases in IL-17 mRNA expression. Remarkably, NAC supplementation attenuated not only the TCE-induced oxidative stress, IL-17 release and mRNA expression, but also the markers of autoimmunity, as evident from decreased levels of ANA, anti-dsDNA and anti-Sm antibodies in the sera. These results provide further support to a role of oxidative stress in TCE-induced autoimmune response. Attenuation of TCE-induced autoimmunity in mice by NAC provides an approach for preventive and/or therapeutic strategies. - Highlights: • TCE led to increased autoantibodies, supporting its potential to induce autoimmunity. • TCE exposure led to increases in lipid perioxidation and protein carbonyls. • TCE exposure resulted in

  12. N-Acetylcysteine protects against trichloroethene-mediated autoimmunity by attenuating oxidative stress

    International Nuclear Information System (INIS)

    Wang, Gangduo; Wang, Jianling; Ma, Huaxian; Ansari, G.A.S.; Khan, M. Firoze


    Exposure to trichloroethene (TCE), a ubiquitous environmental contaminant, is known to induce autoimmunity both in humans and animal models. However, mechanisms underlying TCE-mediated autoimmunity remain largely unknown. Previous studies from our laboratory in MRL +/+ mice suggest that oxidative stress may contribute to TCE-induced autoimmune response. The current study was undertaken to further assess the role of oxidative stress in TCE-induced autoimmunity by supplementing with an antioxidant N-acetylcysteine (NAC). Groups of female MRL +/+ mice were given TCE, NAC or TCE + NAC for 6 weeks (TCE, 10 mmol/kg, i.p., every 4th day; NAC, 250 mg/kg/day through drinking water). TCE exposure led to significant increases in serum levels of anti-nuclear, anti-dsDNA and anti-Sm antibodies. TCE exposure also led to significant induction of anti-malondiadelhyde (MDA)- and anti-hydroxynonenal (HNE)-protein adduct antibodies which were associated with increased ANA in the sera along with increased MDA-/HNE-protein adducts in the livers and kidneys, and increases in protein oxidation (carbonylation) in the sera, livers and kidneys, suggesting an overall increase in oxidative stress. Moreover, TCE exposure also resulted in increased release of IL-17 from splenocytes and increases in IL-17 mRNA expression. Remarkably, NAC supplementation attenuated not only the TCE-induced oxidative stress, IL-17 release and mRNA expression, but also the markers of autoimmunity, as evident from decreased levels of ANA, anti-dsDNA and anti-Sm antibodies in the sera. These results provide further support to a role of oxidative stress in TCE-induced autoimmune response. Attenuation of TCE-induced autoimmunity in mice by NAC provides an approach for preventive and/or therapeutic strategies. - Highlights: • TCE led to increased autoantibodies, supporting its potential to induce autoimmunity. • TCE exposure led to increases in lipid perioxidation and protein carbonyls. • TCE exposure resulted in

  13. N-acetylcysteine protects rats with chronic renal failure from gadolinium-chelate nephrotoxicity.

    Directory of Open Access Journals (Sweden)

    Leonardo Victor Barbosa Pereira

    Full Text Available The aim of this study was to evaluate the effect of Gd-chelate on renal function, iron parameters and oxidative stress in rats with CRF and a possible protective effect of the antioxidant N-Acetylcysteine (NAC. Male Wistar rats were submitted to 5/6 nephrectomy (Nx to induced CRF. An ionic-cyclic Gd (Gadoterate Meglumine was administrated (1.5 mM/KgBW, intravenously 21 days after Nx. Clearance studies were performed in 4 groups of anesthetized animals 48 hours following Gd- chelate administration: 1--Nx (n = 7; 2--Nx+NAC (n = 6; 3--Nx+Gd (n = 7; 4--Nx+NAC+Gd (4.8 g/L in drinking water, initiated 2 days before Gd-chelate administration and maintained during 4 days (n = 6. This group was compared with a control. We measured glomerular filtration rate, GFR (inulin clearance, ml/min/kg BW, proteinuria (mg/24 hs, serum iron (µg/dL; serum ferritin (ng/mL; transferrin saturation (%, TIBC (µg/dL and TBARS (nmles/ml. Normal rats treated with the same dose of Gd-chelate presented similar GFR and proteinuria when compared with normal controls, indicating that at this dose Gd-chelate is not nephrotoxic to normal rats. Gd-chelate administration to Nx-rats results in a decrease of GFR and increased proteinuria associated with a decrease in TIBC, elevation of ferritin serum levels, transferrin oversaturation and plasmatic TBARS compared with Nx-rats. The prophylactic treatment with NAC reversed the decrease in GFR and the increase in proteinuria and all alterations in iron parameters and TBARS induced by Gd-chelate. NAC administration to Nx rat did not modify the inulin clearance and iron kinetics, indicating that the ameliorating effect of NAC was specific to Gd-chelate. These results suggest that NAC can prevent Gd-chelate nephrotoxicity in patients with chronic renal failure.

  14. Use of N-Acetylcysteine in Children with Fulminant Hepatic Failure Caused by Acute Viral Hepatitis

    International Nuclear Information System (INIS)

    Saleem, A. F.; Abbas, Q.; Haque, A.


    Objective: To determine the efficacy of N-acetylcysteine (NAC) in children aged > 1 month to 16 years admitted with Fulminant Hepatic Failure (FHF) secondary to Acute Viral Hepatitis (AVH) in a tertiary care center of a developing country. Study Design: Analytical study. Place and Duration of Study: Department of Paediatrics, The Aga Khan University Hospital, Karachi, Pakistan, from January 2007 to December 2011. Methodology: Medical records of children (> 1 month - 16 years) with FHF admitted with AVH of known etiology who received NAC were reviewed retrospectively. Liver function tests (mean ± SD) at baseline, 24 hours after NAC and before or at the time of discharge/death were recorded and compared via using repeated measures ANOVA(r-ANOVA). Efficacy of NAC is defined in improvement in biochemical markers, liver function test and discharge disposition (survived or died). Mortality associated risk factors were identified by using logistic regression analysis. P-value and 95 percentage confidence interval were recorded. Results: Forty children (mean age was 80 ± 40 months) with FHF secondary to AVH received NAC. Majority were males (n=25; 63 percentage). Vomiting (75 percentage) and jaundice (65 percentage) were the main presenting symptoms, one-third had hypoglycemic, while 40 percentage had altered sensorium at the time of admission. There was significant statistical difference in liver enzymes and prothrombin time on admission comparing at discharge in children received NAC (p < 0.001). Fifteen (38 percentage) children died. Severe vomiting (Odds Ratio (OR) 0.22, 95 percentage Confidence Interval (CI) 0.05 - 0.8), jaundice (OR 9.3, CI 1.1 - 82.6), inotropic support (OR 20.6, CI 3.5 - 118.3) and mechanical ventilation (OR 4.3, CI 1.1 - 16.6) at the time of admission are associated with risk factors for mortality in children with FHF secondary to AVH. Conclusion: NAC used in children with FHF secondary to AVH is associated with markedly improved liver function

  15. The effect of N-acetylcysteine and working memory training on cocaine use, craving and inhibition in regular cocaine users: correspondence of lab assessments and Ecological Momentary Assessment

    NARCIS (Netherlands)

    Schulte, Mieke H. J.; Wiers, Reinout W.; Boendermaker, Wouter J.; Goudriaan, Anna E.; van den Brink, Wim; van Deursen, Denise S.; Friese, Malte; Brede, Emily; Waters, Andrew J.


    Effective treatment for cocaine use disorder should dampen hypersensitive cue-induced motivational processes and/or strengthen executive control. Using a randomized, double-blind, placebo-controlled intervention, the primary aim of this study was to investigate the effect of N-Acetylcysteine (NAC)

  16. N-Acetylcysteine in the Treatment of Pediatric Trichotillomania: A Randomized, Double-Blind, Placebo-Controlled Add-On Trial (United States)

    Bloch, Michael H.; Panza, Kaitlyn E.; Grant, Jon E.; Pittenger, Christopher; Leckman, James F.


    Objective: To examine the efficacy of N-acetylcysteine (NAC) for the treatment of pediatric trichotillomania (TTM) in a double-blind, placebo-controlled, add-on study. Method: A total of 39 children and adolescents aged 8 to 17 years with pediatric trichotillomania were randomly assigned to receive NAC or matching placebo for 12 weeks. Our primary…

  17. Effects of N-Acetylcysteine Addition to University of Wisconsin Solution on the Rate of Ischemia-Reperfusion Injury in Adult Orthotopic Liver Transplant. (United States)

    Aliakbarian, Mohsen; Nikeghbalian, Saman; Ghaffaripour, Sina; Bahreini, Amin; Shafiee, Mohammad; Rashidi, Mohammad; Rajabnejad, Yaser


    One of the main concerns in liver transplant is the prolonged ischemia time, which may lead to primary graft nonfunction or delayed function. N-acetylcysteine is known as a hepato-protective agent in different studies, which may improve human hepatocyte viability in steatotic donor livers. This study investigated whether N-acetylcysteine can decrease the rate of ischemia-reperfusion syndrome and improve short-term outcome in liver transplant recipients. This was a double-blind, randomized, control clinical trial of 115 patients. Between April 2012 and January 2013, patients with orthotopic liver transplant were randomly divided into 2 groups; in 49 cases N-acetylcysteine was added to University of Wisconsin solution as the preservative liquid (experimental group), and in 66 cases standard University of Wisconsin solution was used (control group). We compared postreperfusion hypotension, inotrope requirement before and after portal reperfusion, intermittent arterial blood gas analysis and potassium measurement, pathological review of transplanted liver, in-hospital complications, morbidity, and mortality. There was no significant difference between the groups regarding time to hepatic artery reperfusion, hospital stay, vascular complications, inotrope requirement before and after portal declamping, and blood gas analysis. Hypotension after portal reperfusion was significantly more common in experimental group compared with control group (P = .005). Retransplant and in-hospital mortality were comparable between the groups. Preservation of the liver inside Univer-sity of Wisconsin solution plus N-acetylcysteine did not change the rate of ischemia reperfusion injury and short-term outcome in liver transplant recipients.

  18. Effects of N-acetylcysteine (NAC) supplementation in resuscitation fluids on renal microcirculatory oxygenation, inflammation, and function in a rat model of endotoxemia

    NARCIS (Netherlands)

    Ergin, Bulent; Guerci, Philippe; Zafrani, Lara; Nocken, Frank; Kandil, Asli; Gurel-Gurevin, Ebru; Demirci-Tansel, Cihan; Ince, Can


    Modulation of inflammation and oxidative stress appears to limit sepsis-induced damage in experimental models. The kidney is one of the most sensitive organs to injury during septic shock. In this study, we evaluated the effect of N-acetylcysteine (NAC) administration in conjunction with fluid

  19. The Influence of N-acetylcysteine and Gender-Related Differences on the Radiosensitivity of Mouse Lymphocytes

    International Nuclear Information System (INIS)

    El-Shamy, E.; El-Kabany, H.


    The objective of the present study is to evaluate the possible efficiency of 200 mg/kg body weight N-acetylcysteine (NAC) on the radiosensitivity of mouse lymphocytes considering gender factor. The half of blood sample from mice was exposed to gamma-radiation (2 Gy). The time course of lymphocyte apotosis of irradiated samples was examined in vitro by flow cytometry and compared with lymphocytes from non-irradiated remaining half of samples. Kinetics of radiation-induced apoptosis was similar among groups, which peaked at 8 h. NAC protected irradiated lymphocytes in male mice. Lymphocytes from female mice were highly radiation resistant compared to males and the NAC provided no additional benefit at the doses used in this study. These results highlight that radiation-induced apoptosis is complex and is modified by the radio protector and gender.

  20. Chemical Changes in Nonthermal Plasma-Treated N-Acetylcysteine (NAC) Solution and Their Contribution to Bacterial Inactivation. (United States)

    Ercan, Utku K; Smith, Josh; Ji, Hai-Feng; Brooks, Ari D; Joshi, Suresh G


    In continuation of our previous reports on the broad-spectrum antimicrobial activity of atmospheric non-thermal dielectric barrier discharge (DBD) plasma treated N-Acetylcysteine (NAC) solution against planktonic and biofilm forms of different multidrug resistant microorganisms, we present here the chemical changes that mediate inactivation of Escherichia coli. In this study, the mechanism and products of the chemical reactions in plasma-treated NAC solution are shown. UV-visible spectrometry, FT-IR, NMR, and colorimetric assays were utilized for chemical characterization of plasma treated NAC solution. The characterization results were correlated with the antimicrobial assays using determined chemical species in solution in order to confirm the major species that are responsible for antimicrobial inactivation. Our results have revealed that plasma treatment of NAC solution creates predominantly reactive nitrogen species versus reactive oxygen species, and the generated peroxynitrite is responsible for significant bacterial inactivation.

  1. Risk factors in the development of adverse reactions to N-acetylcysteine in patients with paracetamol poisoning

    DEFF Research Database (Denmark)

    Schmidt, L E; Dalhoff, K


    AIMS: To identify risk factors in the development of side-effects to N-acetylcysteine (NAC) in patients with paracetamol poisoning. METHODS: A retrospective study was carried out based upon the hospital charts of 529 consecutive patients admitted with paracetamol poisoning, all treated with NAC...... 2.9 times (95% CI 2.1, 4.7) more likely to develop side-effects (Chi-square: P = 0.004). Side-effects were of similar severity in asthmatics and nonasthmatics. A history of medical allergy was not a risk factor. Serum paracetamol was lower in patients with side-effects than in those without (Mann......-Whitney: P = 0.00006). CONCLUSIONS: Asthma must be considered a risk factor in the development of side-effects to NAC. However, the side-effects are easily managed and there is no reason to withhold NAC from any patient with paracetamol poisoning. Paracetamol itself seems to offer some protection against...

  2. Efficacy of N-Acetylcysteine Augmentation on Obsessive Compulsive Disorder: A Multicenter Randomized Double Blind Placebo Controlled Clinical Trial

    Directory of Open Access Journals (Sweden)

    Ahmad Ghanizadeh


    Full Text Available Objective: Glutamate is considered a target for treating obsessive-compulsive disorder (OCD. The efficacy and safety of the nutritional supplement of N-Acetylcysteine (NAC as an adjuvant to serotonin reuptake inhibitor (SSRI for treating children and adolescents with OCD has never been examined.Methods: This was a 10-week randomized double-blind placebo-controlled clinical trial with 34 OCD outpatients. The patients received citalopram plus NAC or placebo. Yale-Brown Obsessive-Compulsive Scale (YBOCS and Pediatric Quality of Life Inventory (PedsQL™ were used. Adverse effects were monitored.Results: YBOCS score was not different between the two groups at baseline, but the score was different between the two groups at the end of this trial (P<0.02. The YBOCS score of NAC group significantly decreased from 21.0(8.2 to 11.3(5.7 during this study. However, no statistically significant decrease of YBOCS was found in the placebo group. The Cohen’s d effect size was 0.83.The mean change of score of resistance/control to obsessions in the NAC and placebo groups was 1.8(2.3 and 0.8(2.1, respectively (P = 0.2. However, the mean score of change for resistance/control to compulsion in the NAC and placebo groups was 2.3(1.8 and 0.9(2.3, respectively. Cohen’s d effect size was 0.42.The score of three domains of quality of life significantly decreased in N-Acetylcysteine group during this trial. However, no statistically significant decrease was detected in the placebo group. No serious adverse effect was found in the two groups.Conclusion: This trial suggests that NAC adds to the effect of citalopram in improving resistance/control to compulsions in OCD children and adolescents. In addition, it is well tolerated.

  3. N-acetylcysteine prevents nitrosative stress-associated depression of blood pressure and heart rate in streptozotocin diabetic rats. (United States)

    Nagareddy, Prabhakara Reddy; Xia, Zhengyuan; MacLeod, Kathleen M; McNeill, John H


    Previous studies have indicated that cardiovascular abnormalities such as depressed blood pressure and heart rate occur in streptozotocin (STZ) diabetic rats. Chronic diabetes, which is associated with increased expression of inducible nitric oxide synthase (iNOS) and oxidative stress, may produce peroxynitrite/nitrotyrosine and cause nitrosative stress. We hypothesized that nitrosative stress causes cardiovascular depression in STZ diabetic rats and therefore can be corrected by reducing its formation. Control and STZ diabetic rats were treated orally for 9 weeks with N-acetylcysteine (NAC), an antioxidant and inhibitor of iNOS. At termination, the mean arterial blood pressure (MABP) and heart rate (HR) were measured in conscious rats. Nitrotyrosine and endothelial nitric oxide synthase (eNOS) and iNOS expression were assessed in the heart and mesenteric arteries by immunohistochemistry and Western blot experiments. Untreated diabetic rats showed depressed MABP and HR that was prevented by treatment with NAC. In untreated diabetic rats, levels of 15-F(2t)-isoprostane, an indicator of lipid peroxidation increased, whereas plasma nitric oxide and antioxidant concentrations decreased. Furthermore, decreased eNOS and increased iNOS expression were associated with elevated nitrosative stress in blood vessel and heart tissue of untreated diabetic rats. N-acetylcysteine treatment of diabetic rats not only restored the antioxidant capacity but also reduced the expression of iNOS and nitrotyrosine and normalized the expression of eNOS to that of control rats in heart and superior mesenteric arteries. The results suggest that nitrosative stress depress MABP and HR following diabetes. Further studies are required to elucidate the mechanisms involved in nitrosative stress mediated depression of blood pressure and heart rate.

  4. Prospects of N-Acetylcysteine and Melatonin as Treatments for Tramadol-Induced Renal Toxicity in Albino Rats

    Directory of Open Access Journals (Sweden)

    Elias Adikwu, Bonsome Bokolo


    Full Text Available Background: Tramadol (TD has played an important role in the treatment of pain. However, renal toxicity due to TD abuse is a serious clinical challenge. This study assessed the effects of n-acetylcysteine (NAC and melatonin (MT on TD-induced renal toxicity in albino rats. Methods: Rats were randomized into groups and treated with MT (10mg/kg/day, NAC (10mg/kg/day and TD (15, 30, and 45mg/kg/day respectively. Rats were pretreated with MT (10mg/kg/day and NAC (10mg/kg/day prior to treatment with TD (15, 30, and 45mg/kg/day intraperitonialy for 7days respectively. Rats were sacrificed, serum extracted and evaluated for creatinine, urea and uric acid. The kidneys were evaluated for malondialdehyde (MDA, superoxide dismutase (SOD, catalase, (CAT, and glutathione (GSH levels. Results: Treatment with MT and NAC did not produce significant (P>0.05 effects on serum creatinine, urea, uric acid and kidney MDA, SOD, CAT, and GSH levels when compare to saline control. In contrast, serum creatinine, urea, uric acid and kidney MDA levels were increased while kidney SOD, CAT, and GSH levels were decreased significantly (P<0.05 and in a dose-dependent manner in TD-treated rats. Kidneys of TD-treated rats showed varying degrees of damage which were dose-dependent. However, in all evaluated parameters, TD-induced alterations were abrogated in NAC and MT pretreated rats. Abrogations were most evident in rats pretreated with combined doses of NAC and MT. Conclusion: The present study showed prospects of n-acetylcysteine and melatonin as remedies for tramadol associated renal toxicity.

  5. N-Acetylcysteine Amide Protects Against Oxidative Stress–Induced Microparticle Release From Human Retinal Pigment Epithelial Cells (United States)

    Carver, Kyle A.; Yang, Dongli


    Purpose Oxidative stress is a major factor involved in retinal pigment epithelium (RPE) apoptosis that underlies AMD. Drusen, extracellular lipid- and protein-containing deposits, are strongly associated with the development of AMD. Cell-derived microparticles (MPs) are small membrane-bound vesicles shed from cells. The purpose of this study was to determine if oxidative stress drives MP release from RPE cells, to assess whether these MPs carry membrane complement regulatory proteins (mCRPs: CD46, CD55, and CD59), and to evaluate the effects of a thiol antioxidant on oxidative stress–induced MP release. Methods Retinal pigment epithelium cells isolated from human donor eyes were cultured and treated with hydrogen peroxide (H2O2) to induce oxidative stress. Isolated MPs were fixed for transmission electron microscopy or processed for component analysis by flow cytometry, Western blot analysis, and confocal microscopy. Results Transmission electron microscopy showed that MPs ranged in diameter from 100 to 1000 nm. H2O2 treatment led to time- and dose-dependent elevations in MPs with externalized phosphatidylserine and phosphatidylethanolamine, known markers of MPs. These increases were strongly correlated to RPE apoptosis. Oxidative stress significantly increased the release of mCRP-positive MPs, which were prevented by a thiol antioxidant, N-acetylcysteine amide (NACA). Conclusions This is the first evidence that oxidative stress induces cultured human RPE cells to release MPs that carry mCRPs on their surface. The levels of released MPs are strongly correlated with RPE apoptosis. N-acetylcysteine amide prevents oxidative stress–induced effects. Our findings indicate that oxidative stress reduces mCRPs on the RPE surface through releasing MPs. PMID:26842754

  6. Systematic Review of Human and Animal Studies Examining the Efficacy and Safety of N-Acetylcysteine (NAC) and N-Acetylcysteine Amide (NACA) in Traumatic Brain Injury: Impact on Neurofunctional Outcome and Biomarkers of Oxidative Stress and Inflammation. (United States)

    Bhatti, Junaid; Nascimento, Barto; Akhtar, Umbreen; Rhind, Shawn G; Tien, Homer; Nathens, Avery; da Luz, Luis Teodoro


    No new therapies for traumatic brain injury (TBI) have been officially translated into current practice. At the tissue and cellular level, both inflammatory and oxidative processes may be exacerbated post-injury and contribute to further brain damage. N- acetylcysteine (NAC) has the potential to downregulate both processes. This review focuses on the potential neuroprotective utility of NAC and N -acetylcysteine amide (NACA) post-TBI. Medline, Embase, Cochrane Library, and were searched up to July 2017. Studies that examined clinical and laboratory effects of NAC and NACA post-TBI in human and animal studies were included. Risk of bias was assessed in human and animal studies according to the design of each study (randomized or not). The primary outcome assessed was the effect of NAC/NACA treatment on functional outcome, while secondary outcomes included the impact on biomarkers of inflammation and oxidation. Due to the clinical and methodological heterogeneity observed across studies, no meta-analyses were conducted. Our analyses revealed only three human trials, including two randomized controlled trials (RCTs) and 20 animal studies conducted using standardized animal models of brain injury. The two RCTs reported improvement in the functional outcome post-NAC/NACA administration. Overall, the evidence from animal studies is more robust and demonstrated substantial improvement of cognition and psychomotor performance following NAC/NACA use. Animal studies also reported significantly more cortical sparing, reduced apoptosis, and lower levels of biomarkers of inflammation and oxidative stress. No safety concerns were reported in any of the studies included in this analysis. Evidence from the animal literature demonstrates a robust association for the prophylactic application of NAC and NACA post-TBI with improved neurofunctional outcomes and downregulation of inflammatory and oxidative stress markers at the tissue level. While a growing body of

  7. Systematic Review of Human and Animal Studies Examining the Efficacy and Safety of N-Acetylcysteine (NAC and N-Acetylcysteine Amide (NACA in Traumatic Brain Injury: Impact on Neurofunctional Outcome and Biomarkers of Oxidative Stress and Inflammation

    Directory of Open Access Journals (Sweden)

    Junaid Bhatti


    Full Text Available BackgroundNo new therapies for traumatic brain injury (TBI have been officially translated into current practice. At the tissue and cellular level, both inflammatory and oxidative processes may be exacerbated post-injury and contribute to further brain damage. N-acetylcysteine (NAC has the potential to downregulate both processes. This review focuses on the potential neuroprotective utility of NAC and N-acetylcysteine amide (NACA post-TBI.MethodsMedline, Embase, Cochrane Library, and were searched up to July 2017. Studies that examined clinical and laboratory effects of NAC and NACA post-TBI in human and animal studies were included. Risk of bias was assessed in human and animal studies according to the design of each study (randomized or not. The primary outcome assessed was the effect of NAC/NACA treatment on functional outcome, while secondary outcomes included the impact on biomarkers of inflammation and oxidation. Due to the clinical and methodological heterogeneity observed across studies, no meta-analyses were conducted.ResultsOur analyses revealed only three human trials, including two randomized controlled trials (RCTs and 20 animal studies conducted using standardized animal models of brain injury. The two RCTs reported improvement in the functional outcome post-NAC/NACA administration. Overall, the evidence from animal studies is more robust and demonstrated substantial improvement of cognition and psychomotor performance following NAC/NACA use. Animal studies also reported significantly more cortical sparing, reduced apoptosis, and lower levels of biomarkers of inflammation and oxidative stress. No safety concerns were reported in any of the studies included in this analysis.ConclusionEvidence from the animal literature demonstrates a robust association for the prophylactic application of NAC and NACA post-TBI with improved neurofunctional outcomes and downregulation of inflammatory and oxidative stress markers at

  8. Pre-clinical evaluation of N-acetylcysteine reveals side effects in the mdx mouse model of Duchenne muscular dystrophy. (United States)

    Pinniger, Gavin J; Terrill, Jessica R; Assan, Evanna B; Grounds, Miranda D; Arthur, Peter G


    Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disease associated with increased inflammation and oxidative stress. The antioxidant N-acetylcysteine (NAC) has been proposed as a therapeutic intervention for DMD boys, but potential adverse effects of NAC have not been widely investigated. We used young (6 weeks old) growing mdx mice to investigate the capacity of NAC supplementation (2% in drinking water for 6 weeks) to improve dystrophic muscle function and to explore broader systemic effects of NAC treatment. NAC treatment improved normalised measures of muscle function, and decreased inflammation and oxidative stress, but significantly reduced body weight gain, muscle weight and liver weight. Unexpected significant adverse effects of NAC on body and muscle weights indicate that interpretation of muscle function based on normalised force measures should be made with caution and careful consideration is needed when proposing the use of NAC as a therapeutic treatment for young DMD boys. Duchenne muscular dystrophy (DMD) is a fatal X-linked muscle wasting disease characterised by severe muscle weakness, necrosis, inflammation and oxidative stress. The antioxidant N-acetylcysteine (NAC) has been proposed as a potential therapeutic intervention for DMD boys. We investigated the capacity of NAC to improve dystrophic muscle function in the mdx mouse model of DMD. Young (6 weeks old) mdx and non-dystrophic C57 mice receiving 2% NAC in drinking water for 6 weeks were compared with untreated mice. Grip strength and body weight were measured weekly, before the 12 week old mice were anaesthetised and extensor digitorum longus (EDL) muscles were excised for functional analysis and tissues were sampled for biochemical analyses. Compared to untreated mice, the mean (SD) normalised grip strength was significantly greater in NAC-treated mdx [3.13 (0.58) vs 4.87 (0.78) g body weight (bw) -1 ; P muscles [9.80 (2.27) vs 13.07 (3.37) N cm -2 ; P = 0

  9. N-Acetylcysteine, a glutathione precursor, reverts vascular dysfunction and endothelial epigenetic programming in intrauterine growth restricted guinea pigs. (United States)

    Herrera, Emilio A; Cifuentes-Zúñiga, Francisca; Figueroa, Esteban; Villanueva, Cristian; Hernández, Cherie; Alegría, René; Arroyo-Jousse, Viviana; Peñaloza, Estefania; Farías, Marcelo; Uauy, Ricardo; Casanello, Paola; Krause, Bernardo J


    Intrauterine growth restriction (IUGR) is associated with vascular dysfunction, oxidative stress and signs of endothelial epigenetic programming of the umbilical vessels. There is no evidence that this epigenetic programming is occurring on systemic fetal arteries. In IUGR guinea pigs we studied the functional and epigenetic programming of endothelial nitric oxide synthase (eNOS) (Nos3 gene) in umbilical and systemic fetal arteries, addressing the role of oxidative stress in this process by maternal treatment with N-acetylcysteine (NAC) during the second half of gestation. The present study suggests that IUGR endothelial cells have common molecular markers of programming in umbilical and systemic arteries. Notably, maternal treatment with NAC restores fetal growth by increasing placental efficiency and reverting the functional and epigenetic programming of eNOS in arterial endothelium in IUGR guinea pigs. In humans, intrauterine growth restriction (IUGR) is associated with vascular dysfunction, oxidative stress and signs of endothelial programming in umbilical vessels. We aimed to determine the effects of maternal antioxidant treatment with N-acetylcysteine (NAC) on fetal endothelial function and endothelial nitric oxide synthase (eNOS) programming in IUGR guinea pigs. IUGR was induced by implanting ameroid constrictors on uterine arteries of pregnant guinea pigs at mid gestation, half of the sows receiving NAC in the drinking water (from day 34 until term). Fetal biometry and placental vascular resistance were followed by ultrasound throughout gestation. At term, umbilical arteries and fetal aortae were isolated to assess endothelial function by wire-myography. Primary cultures of endothelial cells (ECs) from fetal aorta, femoral and umbilical arteries were used to determine eNOS mRNA levels by quantitative PCR and analyse DNA methylation in the Nos3 promoter by pyrosequencing. Doppler ultrasound measurements showed that NAC reduced placental vascular resistance

  10. Chronic N-acetylcysteine administration prevents development of hypertension in Nomega-nitro-L-arginine methyl ester-treated rats: the role of reactive oxygen species

    Czech Academy of Sciences Publication Activity Database

    Rauchová, Hana; Pecháňová, O.; Kuneš, Jaroslav; Vokurková, Martina; Dobešová, Zdenka; Zicha, Josef


    Roč. 28, č. 5 (2005), s. 475-482 ISSN 0916-9636 R&D Projects: GA ČR(CZ) GA305/03/0769; GA MZd(CZ) NR7786 Grant - others:VEGA(SK) 02/3185/24; SAV(SK) APVT-51-017902 Institutional research plan: CEZ:AV0Z5011922 Keywords : N-acetylcysteine * nitric oxide synthase * superoxide anions Subject RIV: ED - Physiology Impact factor: 2.786, year: 2005

  11. P2X(7 receptor in the kidneys of diabetic rats submitted to aerobic training or to N-acetylcysteine supplementation [corrected].

    Directory of Open Access Journals (Sweden)

    Adelson M Rodrigues

    Full Text Available Previous studies in our laboratory showed that N-acetylcysteine supplementation or aerobic training reduced oxidative stress and the progression of diabetic nephropathy in rats. The P2X(7 receptor is up-regulated in pathological conditions, such as diabetes mellitus. This up-regulation is related to oxidative stress and induces tissue apoptosis or necrosis. The aim of the present study is to assess the role of P2X(7 receptor in the kidneys of diabetic rats submitted to aerobic training or N-acetylcysteine supplementation. Diabetes was induced in male Wistar rats by streptozotocin (60 mg/kg, i.v. and the training was done on a treadmill; N-acetylcysteine was given in the drinking water (600 mg/L. By confocal microscopy, as compared to control, the kidneys of diabetic rats showed increased P2 × 7 receptor expression and a higher activation in response to 2'(3'-O-(4-benzoylbenzoyl adenosine5'-triphosphate (specific agonist and adenosine triphosphate (nonspecific agonist (all p<0.05. All these alterations were reduced in diabetic rats treated with N-acetylcysteine, exercise or both. We also observed measured proteinuria and albuminuria (early marker of diabetic nephropathy in DM groups. Lipoperoxidation was strongly correlated with P2X(7 receptor expression, which was also correlated to NO•, thus associating this receptor to oxidative stress and kidney lesion. We suggest that P2X(7 receptor inhibition associated with the maintenance of redox homeostasis could be useful as coadjuvant treatment to delay the progression of diabetic nephropathy.

  12. P2X(7) receptor in the kidneys of diabetic rats submitted to aerobic training or to N-acetylcysteine supplementation [corrected]. (United States)

    Rodrigues, Adelson M; Bergamaschi, Cassia T; Fernandes, Maria Jose S; Paredes-Gamero, Edgar J; Buri, Marcus V; Curi, Marcus V; Ferreira, Alice T; Araujo, Sergio R R; Punaro, Giovana R; Maciel, Fabiane R; Nogueira, Guilherme B; Higa, Elisa M S


    Previous studies in our laboratory showed that N-acetylcysteine supplementation or aerobic training reduced oxidative stress and the progression of diabetic nephropathy in rats. The P2X(7 receptor is up-regulated in pathological conditions, such as diabetes mellitus. This up-regulation is related to oxidative stress and induces tissue apoptosis or necrosis. The aim of the present study is to assess the role of P2X(7) receptor in the kidneys of diabetic rats submitted to aerobic training or N-acetylcysteine supplementation. Diabetes was induced in male Wistar rats by streptozotocin (60 mg/kg, i.v.) and the training was done on a treadmill; N-acetylcysteine was given in the drinking water (600 mg/L). By confocal microscopy, as compared to control, the kidneys of diabetic rats showed increased P2 × 7 receptor expression and a higher activation in response to 2'(3')-O-(4-benzoylbenzoyl) adenosine5'-triphosphate (specific agonist) and adenosine triphosphate (nonspecific agonist) (all p<0.05). All these alterations were reduced in diabetic rats treated with N-acetylcysteine, exercise or both. We also observed measured proteinuria and albuminuria (early marker of diabetic nephropathy) in DM groups. Lipoperoxidation was strongly correlated with P2X(7) receptor expression, which was also correlated to NO•, thus associating this receptor to oxidative stress and kidney lesion. We suggest that P2X(7) receptor inhibition associated with the maintenance of redox homeostasis could be useful as coadjuvant treatment to delay the progression of diabetic nephropathy.

  13. Contribution of neuronal NO synthase to N-acetylcysteine-induced increase of NO synthase activity in the brain of normotensive and hypertensive rats

    Czech Academy of Sciences Publication Activity Database

    Pecháňová, Olga; Kuneš, Jaroslav; Dobešová, Zdenka; Vranková, S.; Zicha, Josef


    Roč. 60, č. 4 (2009), s. 21-25 ISSN 0867-5910 R&D Projects: GA MŠk(CZ) 1M0510; GA ČR(CZ) GA305/08/0139 Grant - others:VEGA(SK) 2/0178/09; APVV(SK) 0538-07 Institutional research plan: CEZ:AV0Z50110509 Keywords : N-acetylcysteine * S-methyl-L-thiocitrulline * spontaneous hypertension Subject RIV: ED - Physiology Impact factor: 1.489, year: 2009

  14. N-acetylcysteine normalizes the urea cycle and DNA repair in cells from patients with Batten disease. (United States)

    Kim, June-Bum; Lim, Nary; Kim, Sung-Jo; Heo, Tae-Hwe


    Batten disease is an inherited disorder characterized by early onset neurodegeneration due to the mutation of the CLN3 gene. The function of the CLN3 protein is not clear, but an association with oxidative stress has been proposed. Oxidative stress and DNA damage play critical roles in the pathogenesis of neurodegenerative diseases. Antioxidants are of interest because of their therapeutic potential for treating neurodegenerative diseases. We tested whether N-acetylcysteine (NAC), a well-known antioxidant, improves the pathology of cells from patients with Batten disease. At first, the expression levels of urea cycle components and DNA repair enzymes were compared between Batten disease cells and normal cells. We used both mRNA expression levels and Western blot analysis. We found that carbamoyl phosphate synthetase 1, an enzyme involved in the urea cycle, 8-oxoguanine DNA glycosylase 1 and DNA polymerase beta, enzymes involved in DNA repair, were expressed at higher levels in Batten disease cells than in normal cells. The treatment of Batten disease cells with NAC for 48 h attenuated activities of the urea cycle and of DNA repair, as indicated by the substantially decreased expression levels of carbamoyl phosphate synthetase 1, 8-oxoguanine DNA glycosylase 1 and DNA polymerase beta proteins compared with untreated Batten cells. NAC may serve in alleviating the burden of urea cycle and DNA repair processes in Batten disease cells. We propose that NAC may have beneficial effects in patients with Batten disease. Copyright © 2012 John Wiley & Sons, Ltd.

  15. N-acetylcysteine selectively antagonizes the activity of imipenem in Pseudomonas aeruginosa by an OprD-mediated mechanism. (United States)

    Rodríguez-Beltrán, Jerónimo; Cabot, Gabriel; Valencia, Estela Ynés; Costas, Coloma; Bou, German; Oliver, Antonio; Blázquez, Jesús


    The modulating effect of N-acetylcysteine (NAC) on the activity of different antibiotics has been studied in Pseudomonas aeruginosa. Our results demonstrate that, in contrast to previous reports, only the activity of imipenem is clearly affected by NAC. MIC and checkerboard determinations indicate that the NAC-based modulation of imipenem activity is dependent mainly on OprD. SDS-PAGE of outer membrane proteins (OMPs) after NAC treatments demonstrates that NAC does not modify the expression of OprD, suggesting that NAC competitively inhibits the uptake of imipenem through OprD. Similar effects on imipenem activity were obtained with P. aeruginosa clinical isolates. Our results indicate that imipenem-susceptible P. aeruginosa strains become resistant upon simultaneous treatment with NAC and imipenem. Moreover, the generality of the observed effects of NAC on antibiotic activity was assessed with two additional bacterial species, Escherichia coli and Acinetobacter baumannii. Caution should be taken during treatments, as the activity of imipenem may be modified by physiologically attainable concentrations of NAC, particularly during intravenous and nebulized regimes. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  16. Evaluation of N-acetylcysteine and methylprednisolone as therapies for oxygen and acrolein-induced lung damage

    Energy Technology Data Exchange (ETDEWEB)

    Critchley, J.A.J.H. (Univ. of Edinburgh (England)); Beeley, J.M.; Clark, R.J.; Buchanan, J.D. (Royal Naval Hospital Hoslar, Gosport (England)); Summerfield, M.; Bell, S. (Admiralty Research Establishment, Alverstoke (England)); Spurlock, M.S.; Edginton, J.A.G. (Chemical Defence Establishment, Porton Down (England))


    Reactive oxidizing species are implicated in the etiology of a range of inhalational pulmonary injuries. Consequently, various free radical scavengers have been tested as potential prophylactic agents. The sulfydryl compound, N-acetylcysteine (NAC) is the only such compound clinically available for use in realistic dosages, and it is well established as an effective antidote for the hepatic and renal toxicity of paracetamol. Another approach in pulmonary injury prophylaxis is methylprednisolone therapy. The authors evaluated NAC and methylprednisolone in two rats models of inhalation injury: 40-hr exposure to >97% oxygen at 1.1 bar and 15-min exposure to acrolein vapor (210 ppm). The increases in lung wet/dry weight ratios, seen with both oxygen and acrolein toxicity were reduced with both treatments. However, with oxygen, NAC therapy was associated with considerably increased mortality and histological changes. Furthermore, IP NAC administration resulted in large volumes of ascitic fluid. With acrolein, IV, NAC had no significant effect on mortality or pulmonary histological damage. Methylprednisolone had no beneficial effects on either the mortality or histological damage observed in either toxicity model. They caution against the ad hoc use of NAC in the management of inhalational pulmonary injury.

  17. Prolonged treatment with N-acetylcysteine and L-arginine restores gonadal function in patients with polycystic ovary syndrome. (United States)

    Masha, A; Manieri, C; Dinatale, S; Bruno, G A; Ghigo, E; Martina, V


    Nitric oxide (NO) plays a wide spectrum of biological actions including a positive role in oocyte maturation and ovulation. Free radicals levels have been shown elevated in polycystic ovary syndrome (PCOS) and therefore would be responsible for quenching NO that, in turn, would play a role in determining oligo- or amenorrhea connoting PCOS. Eight patients with PCOS displaying oligo-amenorrhea from at least 1 yr underwent a combined treatment with N-acetylcysteine (NAC) (1200 mg/die) plus L-arginine (ARG) (1600 mg/die) for 6 months. Menstrual function, glucose and insulin levels, and, in turn, homeostasis model assessment (HOMA) index were monitored. Menstrual function was at some extent restored as indicated by the number of uterine bleedings under treatment (3.00, 0.18-5.83 vs 0.00, 0.00-0.83; p<0.02). Also, a well-defined biphasic pattern in the basal body temperature suggested ovulatory cycles. The HOMA index decreased under treatment (2.12, 1.46-4.42 vs 3.48, 1.62-5.95; p<0.05). In conclusion, this preliminary, open study suggests that prolonged treatment with NAC+ARG might restore gonadal function in PCOS. This effect seems associated to an improvement in insulin sensitivity.

  18. The efficacy of N-acetylcysteine as an adjunctive treatment in bipolar depression: an open label trial. (United States)

    Berk, Michael; Dean, Olivia; Cotton, Sue M; Gama, Clarissa S; Kapczinski, Flavio; Fernandes, Brisa S; Kohlmann, Kristy; Jeavons, Susan; Hewitt, Karen; Allwang, Christine; Cobb, Heidi; Bush, Ashley I; Schapkaitz, Ian; Dodd, Seetal; Malhi, Gin S


    Evidence is accumulating to support the presence of redox dysregulation in a number of psychiatric disorders, including bipolar disorder. This dysregulation may be amenable to therapeutic intervention. Glutathione is the predominant non-enzymatic intracellular free radical scavenger in the brain, and the most generic of all endogenous antioxidants in terms of action. N-acetylcysteine (NAC) is a glutathione precursor that effectively replenishes brain glutathione. Given the failure of almost all modern trials of antidepressants in bipolar disorder to demonstrate efficacy, and the limited efficacy of mood stabilisers in the depressive phase of the disorder, this is a major unmet need. This study reports data on the treatment of 149 individuals with moderate depression during the 2 month open label phase of a randomised placebo controlled clinical trial of the efficacy of 1g BID of NAC that examined the use of NAC as a maintenance treatment for bipolar disorder. In this trial, the estimated mean baseline Bipolar Depression Rating Scale (BDRS) score was 19.7 (SE=0.8), and the mean BDRS score at the end of the 8 week open label treatment phase was 11.1 (SE=0.8). This reduction was statistically significant (pdepression scores with NAC treatment. Large placebo controlled trials of acute bipolar depression are warranted. Copyright © 2011 Elsevier B.V. All rights reserved.

  19. Storing red blood cells with vitamin C and N-acetylcysteine prevents oxidative stress-related lesions: a metabolomics overview. (United States)

    Pallotta, Valeria; Gevi, Federica; D'alessandro, Angelo; Zolla, Lello


    Recent advances in red blood cell metabolomics have paved the way for further improvements of storage solutions. In the present study, we exploited a validated high performance liquid chromatography-mass spectrometry analytical workflow to determine the effects of vitamin C and N-acetylcysteine supplementation (anti-oxidants) on the metabolome of erythrocytes stored in citrate-phosphate-dextrose saline-adenine-glucose-mannitol medium under blood bank conditions. We observed decreased energy metabolism fluxes (glycolysis and pentose phosphate pathway). A tentative explanation of this phenomenon could be related to the observed depression of the uptake of glucose, since glucose and ascorbate are known to compete for the same transporter. Anti-oxidant supplementation was effective in modulating the redox poise, through the promotion of glutathione homeostasis, which resulted in decreased haemolysis and less accumulation of malondialdehyde and oxidation by-products (including oxidized glutathione and prostaglandins). Anti-oxidants improved storage quality by coping with oxidative stress at the expense of glycolytic metabolism, although reservoirs of high energy phosphate compounds were preserved by reduced cyclic AMP-mediated release of ATP.

  20. Adjunctive N-acetylcysteine in depression: exploration of interleukin-6, C-reactive protein and brain-derived neurotrophic factor. (United States)

    Hasebe, Kyoko; Gray, Laura; Bortolasci, Chiara; Panizzutti, Bruna; Mohebbi, Mohammadreza; Kidnapillai, Srisaiyini; Spolding, Briana; Walder, Ken; Berk, Michael; Malhi, Gin; Dodd, Seetal; Dean, Olivia M


    This study aimed to explore effects of adjunctive N-acetylcysteine (NAC) treatment on inflammatory and neurogenesis markers in unipolar depression. We embarked on a 12-week clinical trial of NAC (2000 mg/day compared with placebo) as an adjunctive treatment for unipolar depression. A follow-up visit was conducted 4 weeks following the completion of treatment. We collected serum samples at baseline and the end of the treatment phase (week 12) to determine changes in interleukin-6 (IL6), C-reactive protein (CRP) and brain-derived neurotrophic factor (BDNF) following NAC treatment. NAC treatment significantly improved depressive symptoms on the Montgomery-Asberg Depression Rating Scale (MADRS) over 16 weeks of the trial. Serum levels of IL6 were associated with reductions of MADRS scores independent of treatment response. However, we found no significant changes in IL6, CRP and BDNF levels following NAC treatment. Overall, this suggests that our results failed to support the hypothesis that IL6, CRP and BDNF are directly involved in the therapeutic mechanism of NAC in depression. IL6 may be a useful marker for future exploration of treatment response.

  1. The effects of N-acetylcysteine on cocaine reward and seeking behaviors in a rat model of depression. (United States)

    Frankowska, Małgorzata; Jastrzębska, Joanna; Nowak, Ewa; Białko, Magdalena; Przegaliński, Edmund; Filip, Małgorzata


    Depression and substance-abuse (e.g., cocaine) disorders are common concurrent diagnoses. In the present study, we combined bilateral olfactory bulbectomy (OBX) with a variety of procedures of intravenous cocaine self-administration and extinction/reinstatement in rats. We also investigated the effects of N-acetylcysteine (NAC) on rewarding and seeking behaviors for cocaine in OBX rats and compared the drug's effects in sham-operated control animals (SHAM). The occurrence of depressive symptoms before introduction to cocaine self-administration enhanced subsequent cocaine-seeking behaviors but did not significantly influence cocaine's rewarding properties or extinction training. NAC (25-100mg/kg) given acutely or repeatedly did not alter the co-occurrence of cocaine reward and depression but effectively reduced the cocaine-seeking behavior observed in both phenotypes. Our results indicate that depression behavior is linked to more pronounced drug craving and a higher propensity to relapse in rats. We also show the lack of efficacy of repeated NAC treatment on SHAM or OBX animals in terms of cocaine self-administration, while the drug was an effective blocker of cocaine-seeking behavior in both studied phenotypes, with a more pronounced drug effect observed in OBX animals. The last finding demonstrates the potential clinical utility of NAC to reduce cocaine seeking enhanced by co-existing depression. Copyright © 2014 Elsevier B.V. All rights reserved.

  2. The efficacy of adjunctive N-acetylcysteine in major depressive disorder: a double-blind, randomized, placebo-controlled trial. (United States)

    Berk, Michael; Dean, Olivia M; Cotton, Sue M; Jeavons, Susan; Tanious, Michelle; Kohlmann, Kristy; Hewitt, Karen; Moss, Kirsteen; Allwang, Christine; Schapkaitz, Ian; Robbins, Jenny; Cobb, Heidi; Ng, Felicity; Dodd, Seetal; Bush, Ashley I; Malhi, Gin S


    Major depressive disorder (MDD) is one of the most common psychiatric disorders, conferring considerable individual, family, and community burden. To date, treatments for MDD have been derived from the monoamine hypothesis, and there is a paucity of emerging antidepressants, especially with novel mechanisms of action and treatment targets. N-acetylcysteine (NAC) is a redox-active glutathione precursor that decreases inflammatory cytokines, modulates glutamate, promotes neurogenesis, and decreases apoptosis, all of which contribute to the neurobiology of depression. Participants with a current episode of MDD diagnosed according to DSM-IV-TR criteria (N = 252) were treated with NAC or placebo in addition to treatment as usual for 12 weeks and were followed to 16 weeks. Data were collected between 2007 and 2011. The omnibus interaction between group and visit for the Montgomery-Asberg Depression Rating Scale (MADRS), the primary outcome measure, was not significant (F₁,₅₂₀.₉ = 1.98, P = .067), and the groups did not separate at week 12 (t₃₆₀.₃ = -1.12, P = .265). However, at week 12, the scores on the Longitudinal Interval Follow-Up Evaluation-Range of Impaired Functioning Tool (LIFE-RIFT) differed from placebo (P = .03). Among participants with a MADRS score ≥ 25, NAC separated from placebo at weeks 6, 8, 12, and 16 (P depression pathogenesis, principally oxidative and inflammatory stress and glutamate, although definitive confirmation remains necessary. Identifier: ACTRN12607000134426. © Copyright 2014 Physicians Postgraduate Press, Inc.

  3. N-acetylcysteine for major mental disorders: a systematic review and meta-analysis of randomized controlled trials. (United States)

    Zheng, W; Zhang, Q-E; Cai, D-B; Yang, X-H; Qiu, Y; Ungvari, G S; Ng, C H; Berk, M; Ning, Y-P; Xiang, Y-T


    This systematic review and meta-analysis of randomized controlled trials (RCTs) examined the efficacy and safety of adjunctive N-acetylcysteine (NAC), an antioxidant drug, in treating major depressive disorder (MDD), bipolar disorder, and schizophrenia. The PubMed, Cochrane Library, PsycINFO, CNKI, CBM, and WanFang databases were independently searched and screened by two researchers. Standardized mean differences (SMDs), risk ratios, and their 95% confidence intervals (CIs) were computed. Six RCTs (n = 701) of NAC for schizophrenia (three RCTs, n = 307), bipolar disorder (two RCTs, n = 125), and MDD (one RCT, n = 269) were identified and analyzed as separate groups. Adjunctive NAC significantly improved total psychopathology (SMD = -0.74, 95% CI: -1.43, -0.06; I 2 = 84%, P = 0.03) in schizophrenia, but it had no significant effect on depressive and manic symptoms as assessed by the Young Mania Rating Scale in bipolar disorder and only a small effect on major depressive symptoms. Adverse drug reactions to NAC and discontinuation rates between the NAC and control groups were similar across the three disorders. Adjunctive NAC appears to be a safe treatment that has efficacy for schizophrenia, but not for bipolar disorder or MDD. Further higher quality RCTs are warranted to determine the role of adjunctive NAC in the treatment of major psychiatric disorders. © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  4. Protective effect of N-Acetylcysteine against ethanol-induced gastric ulcer: a pharmacological assessment in mice

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    Ausama Ayoob Jaccob


    Aim: Since there is an increasing need for gastric ulcer therapies with optimum benefit-risk profile. This study was conducted to investigate gastro-protective effects of N-Acetylcysteine (NAC against ethanol-induced gastric ulcer models in mice. Materials and Methods: Forty-two mice were allocated into six groups consisting of 7 mice each. Groups 1 (normal control and 2 (ulcer control received distilled water at a dose of 10 ml/kg, groups 3, 4 and 5 were given NAC at doses 100, 300 and 500 mg/kg, respectively, and the 6th group received ranitidine (50 mg/kg. All drugs administered orally once daily for 7 days, on the 8th day absolute ethanol (7 ml/kg was administrated orally to all mice to induce the acute ulcer except normal control group. Then 3 h after, all animals were sacrificed then consequently the stomachs were excised for examination. Results: NAC administration at the tested doses showed a dose-related potent gastro-protective effect with significant increase in curative ratio, PH of gastric juice and mucus content viscosity seen with the highest dose of NAC and it is comparable with that observed in ranitidine group. Conclusion: The present findings demonstrate that, oral NAC shows significant gastro-protective effects comparable to ranitidine confirmed by antisecretory, cytoprotective, histological and biochemical data but the molecular mechanisms behind such protection are complex. [J Intercult Ethnopharmacol 2015; 4(2.000: 90-95

  5. A Promise in the Treatment of Endometriosis: An Observational Cohort Study on Ovarian Endometrioma Reduction by N-Acetylcysteine

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    Maria Grazia Porpora


    Full Text Available Urged by the unmet medical needs in endometriosis treatment, often with undesirable side effects, and encouraged by N-acetylcysteine (NAC efficacy in an animal model of endometriosis and by the virtual absence of toxicity of this natural compound, we performed an observational cohort study on ovarian endometriosis. NAC treatment or no treatment was offered to 92 consecutive Italian women referred to our university hospital with ultrasound confirmed diagnosis of ovarian endometriosis and scheduled to undergo laparoscopy 3 months later. According to patients acceptance or refusal, NAC-treated and untreated groups finally comprised 73 and 72 endometriomas, respectively. After 3 months, within NAC-treated patients cyst mean diameter was slightly reduced (-1.5 mm versus a significant increase (+6.6 mm in untreated patients (P=0.001. Particularly, during NAC treatment, more cysts reduced and fewer cysts increased their size. Our results are better than those reported after hormonal treatments. Twenty-four NAC-treated patients—versus 1 within controls—cancelled scheduled laparoscopy due to cysts decrease/disappearance and/or relevant pain reduction (21 cases or pregnancy (1 case. Eight pregnancies occurred in NAC-treated patients and 6 in untreated patients. We can conclude that NAC actually represents a simple effective treatment for endometriosis, without side effects, and a suitable approach for women desiring a pregnancy.

  6. Paracetamol (acetaminophen) attenuates in vitro mast cell and peripheral blood mononucleocyte cell histamine release induced by N-acetylcysteine. (United States)

    Coulson, James; Thompson, John Paul


    The treatment of acute paracetamol (acetaminophen) poisoning with N-acetylcysteine (NAC) is frequently complicated by an anaphylactoid reaction to the antidote. The mechanism that underlies this reaction is unclear. We used the human mast cell line 1 (HMC-1) and human peripheral blood mononucleocytes (PBMCs) to investigate the effects of NAC and paracetamol on histamine secretion in vitro. HMC-1 and human PBMCs were incubated in the presence of increasing concentrations of NAC +/- paracetamol. Cell viability was determined by the Trypan Blue Assay, and histamine secretion was measured by ELISA. NAC was toxic to HMC-1 cells at 100 mg/mL and to PBMCs at 67 mg/mL. NAC increased HMC-1 and PBMC histamine secretion at concentrations of NAC from 20 to 50 mg/mL and 2.5 to 100 mg/mL, respectively. NAC-induced histamine secretion by both cell types was reduced by co-incubation with 2.5 mg/mL of paracetamol. Paracetamol (acetaminophen) is capable of modifying histamine secretion in vitro. This may explain the clinical observation of a lower incidence of adverse reactions to NAC in vivo when higher concentrations of paracetamol are present than when paracetamol concentrations are low. Paracetamol (acetaminophen) attenuates in vitro mast cell and PBMC cell histamine release induced by NAC.

  7. N-Acetylcysteine treatment of dystrophic mdx mice results in protein thiol modifications and inhibition of exercise induced myofibre necrosis. (United States)

    Terrill, Jessica R; Radley-Crabb, Hannah G; Grounds, Miranda D; Arthur, Peter G


    Oxidative stress is implicated as a factor that increases necrosis of skeletal muscles in Duchenne Muscular Dystrophy (DMD) and the dystrophic mdx mouse. Consequently, drugs that minimize oxidative stress are potential treatments for muscular dystrophy. This study examined the in vivo benefits to mdx mice of an antioxidant treatment with the cysteine precursor N-acetylcysteine (NAC), administered in drinking water. NAC was completely effective in preventing treadmill exercise-induced myofibre necrosis (assessed histologically) and the increased blood creatine kinase levels (a measure of sarcolemma leakiness) following exercise were significantly lower in the NAC treated mice. While NAC had no effect on malondialdehyde level or protein carbonylation (two indicators of irreversible oxidative damage), treatment with NAC for one week significantly decreased the oxidation of glutathione and protein thiols, and enhanced muscle protein thiol content. These data provide in vivo evidence for protective benefits of NAC treatment on dystropathology, potentially via protein thiol modifications. Copyright © 2011 Elsevier B.V. All rights reserved.

  8. Successful use of N-acetylcysteine to treat severe hepatic injury caused by a dietary fitness supplement. (United States)

    El Rahi, Cynthia; Thompson-Moore, Nathaniel; Mejia, Patricia; De Hoyos, Patricio


    In the absence of adequate premarketing efficacy and safety evaluations, adverse events from over-the-counter supplements are emerging as a public health concern. Specifically, bodybuilding products are being identified as a frequent cause of drug-induced liver injury. We present a case of a 20-year-old Hispanic male who presented with acute nausea and vomiting accompanied by severe right upper quadrant abdominal pain, shivering, and shortness of breath. Laboratory data pointed to mixed cholestatic and hepatocellular damage, and after exclusion of known alternate etiologies, the patient was diagnosed with acute drug-induced liver injury secondary to the use of "Friction," a bodybuilding supplement. Treatment with N-acetylcysteine (NAC) 20% oral solution was initiated empirically at a dose of 4000 mg [DOSAGE ERROR CORRECTED] (70 mg/kg) every 4 hours and was continued once the diagnosis was made. Within 48 hours of admission to our hospital, the patient began to show clinical resolution of right abdominal pain and tolerance to oral diet associated with a significant decline toward normal in his liver function tests and coagulopathy. The WHO-UMC causality assessment system suggested a "certain causality" between exposure to the supplement and the acute liver injury. In the event of suspected drug-induced liver injury, treatment with NAC should be considered given its favorable risk-benefit profile. © 2015 Pharmacotherapy Publications, Inc.

  9. Effect of chitosan-N-acetylcysteine conjugate in a mouse model of botulinum toxin B-induced dry eye. (United States)

    Hongyok, Teeravee; Chae, Jemin J; Shin, Young Joo; Na, Daero; Li, Li; Chuck, Roy S


    To evaluate the effect of a thiolated polymer lubricant, chitosan-N-acetylcysteine conjugate (C-NAC), in a mouse model of dry eye. Eye drops containing 0.5% C-NAC, 0.3% C-NAC, a vehicle (control group), artificial tears, or fluorometholone were applied in a masked fashion in a mouse model of induced dry eye from 3 days to 4 weeks after botulinum toxin B injection. Corneal fluorescein staining was periodically recorded. Real-time reverse transcriptase-polymerase chain reaction and immunofluorescence staining were performed at the end of the study to evaluate inflammatory cytokine expressions. Mice treated with C-NAC, 0.5%, and fluorometholone showed a downward trend that was not statistically significant in corneal staining compared with the other groups. Chitosan-NAC formulations, fluorometholone, and artificial tears significantly decreased IL-1beta (interleukin 1beta), IL-10, IL-12alpha, and tumor necrosis factor alpha expression in ocular surface tissues. The botulinum toxin B-induced dry eye mouse model is potentially useful in evaluating new dry eye treatment. Evaluation of important molecular biomarkers suggests that C-NAC may impart some protective ocular surface properties. However, clinical data did not indicate statistically significant improvement of tear production and corneal staining in any of the groups tested. Topically applied C-NAC might protect the ocular surface in dry eye syndrome, as evidenced by decreased inflammatory cytokine expression.

  10. N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection

    International Nuclear Information System (INIS)

    Wang, Xinyi; Lin, Ruoyun; Xu, Zhihan; Huang, Hongduan; Li, Limei; Liu, Feng; Li, Na; Yang, Xiaoda


    Graphical abstract: -- Highlights: •A new method for nanomolar NAC determination with LOD of 50 nM was reported. •The combined mechanism for NAC quenching with static dominating was suggested. •DNA-Ag NC structure changed with addition of NAC, proved by spectroscopic studies. -- Abstract: In this work, we reported a new, simple and sensitive method for determination of N-acetylcysteine (NAC) based on quenching of the red fluorescence of oligonuleotide-protected silver nanoculsters (Ag NCs) with the quantum yield of 68.3 ± 0.3%. This method was successfully used for the assay of NAC granules presenting a linear range from 100 nM to 1200 nM (LOD of 50 nM) with minimal interferences from potential coexisting substances. It is for the first time that quenching performance of the thiol-containing compound was found to follow a non-linear Stern–Volmer profile, indicative of a complicated quenching mechanism with static quenching dominating, in which DNA-template of Ag NCs was partly replaced by NAC, as elucidated by spectral investigations. This study extended the analytical application of silver nanoclusters as well as provided a more insightful understanding of the quenching mechanism of thiol-compounds on the fluorescence of Ag NCs

  11. N-Acetylcysteine enhances the action of anti-inflammatory drugs as suppressors of prostaglandin production in monocytes

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    Erica Hoffer


    Full Text Available The anti-inflammatory effect of non-steroidal anti-inflammatory drugs (NSAIDs is associated with inhibition of cyclooxygenase (COX, the rate-limiting enzyme responsible for the synthesis of prostaglandins. Since oxygen free radicals can act as second cellular messengers, especially to modulate the metabolism of arachidonic acid and the prostaglandin tract, it seems plausible that antioxidants might affect the production of prostaglandin by activated cells. This research is focused on the effect of the antioxidant N-acetylcysteine (NAC on the inhibition of prostaglandin E2 formation in activated monocytes by specific and non-specific COX inhibitors. We found that lipopolysaccharide-induced prostaglandin E2 formation was significantly reduced by rofecoxib and by diclofenac, two NSAIDs. Addition of NAC to each of these drugs enhanced the effect of the NSAIDs. These results suggest that one might expect either a potentiation of the anti-inflammatory effect of COX inhibitors by their simultaneous administration with NAC, or obtaining the same anti-inflammatory at lower drug levels.

  12. N-Acetylcysteine Prevents Hypertension via Regulation of the ADMA-DDAH Pathway in Young Spontaneously Hypertensive Rats

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    Nai-Chia Fan


    Full Text Available Asymmetric dimethylarginine (ADMA reduces nitric oxide (NO, thus causing hypertension. ADMA is metabolized by dimethylarginine dimethylaminohydrolase (DDAH, which can be inhibited by oxidative stress. N-Acetylcysteine (NAC, an antioxidant, can facilitate glutathione (GSH synthesis. We aimed to determine whether NAC can prevent hypertension by regulating the ADMA-DDAH pathway in spontaneously hypertensive rats (SHR. Rats aged 4 weeks were assigned into 3 groups (n=8/group: control Wistar Kyoto rats (WKY, SHR, and SHR receiving 2% NAC in drinking water. All rats were sacrificed at 12 weeks of age. SHR had higher blood pressure than WKY, whereas NAC-treated animals did not. SHR had elevated plasma ADMA levels, which was prevented by NAC therapy. SHR had lower renal DDAH activity than WKY, whereas NAC-treated animals did not. Renal superoxide production was higher in SHR than in WKY, whereas NAC therapy prevented it. NAC therapy was also associated with higher GSH-to-oxidized GSH ratio in SHR kidneys. Moreover, NAC reduced oxidative stress damage in SHR. The observed antihypertensive effects of NAC in young SHR might be due to restoration of DDAH activity to reduce ADMA, leading to attenuation of oxidative stress. Our findings highlight the impact of NAC on the development of hypertension by regulating ADMA-DDAH pathway.

  13. Inhibitory Effect on Cerebral Inflammatory Response following Traumatic Brain Injury in Rats: A Potential Neuroprotective Mechanism of N-Acetylcysteine

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    Gang Chen


    Full Text Available Although N-acetylcysteine (NAC has been shown to be neuroprotective for traumatic brain injury (TBI, the mechanisms for this beneficial effect are still poorly understood. Cerebral inflammation plays an important role in the pathogenesis of secondary brain injury after TBI. However, it has not been investigated whether NAC modulates TBI-induced cerebral inflammatory response. In this work, we investigated the effect of NAC administration on cortical expressions of nuclear factor kappa B (NF-κB and inflammatory proteins such as interleukin-1β (IL-1β, tumor necrosis factor-α (TNF-α, interleukin-6 (IL-6, and intercellular adhesion molecule-1 (ICAM-1 after TBI. As a result, we found that NF-κB, proinflammatory cytokines, and ICAM-1 were increased in all injured animals. In animals given NAC post-TBI, NF-κB, IL-1β, TNF-α, and ICAM-1 were decreased in comparison to vehicle-treated animals. Measures of IL-6 showed no change after NAC treatment. NAC administration reduced brain edema, BBB permeability, and apoptotic index in the injured brain. The results suggest that post-TBI NAC administration may attenuate inflammatory response in the injured rat brain, and this may be one mechanism by which NAC ameliorates secondary brain damage following TBI.

  14. N-Acetylcysteine for Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis of Randomized Controlled Clinical Trials

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    Divyesh Thakker


    Full Text Available Objective. To review the benefits and harms of N-acetylcysteine (NAC in women with polycystic ovary syndrome (PCOS. Method. Literature search was conducted using the bibliographic databases, MEDLINE (Ovid, CINAHL, EMBASE, Scopus, PsyInfo, and PROQUEST (from inception to September 2013 for the studies on women with PCOS receiving NAC. Results. Eight studies with a total of 910 women with PCOS were randomized to NAC or other treatments/placebo. There were high risk of selection, performance, and attrition bias in two studies and high risk of reporting bias in four studies. Women with NAC had higher odds of having a live birth, getting pregnant, and ovulation as compared to placebo. However, women with NAC were less likely to have pregnancy or ovulation as compared to metformin. There was no significant difference in rates of the miscarriage, menstrual regulation, acne, hirsutism, and adverse events, or change in body mass index, testosterone, and insulin levels with NAC as compared to placebo. Conclusions. NAC showed significant improvement in pregnancy and ovulation rate as compared to placebo. The findings need further confirmation in well-designed randomized controlled trials to examine clinical outcomes such as live birth rate in longer follow-up periods. Systematic review registration number is CRD42012001902.

  15. Spray-dried mucoadhesives for intravesical drug delivery using N-acetylcysteine- and glutathione-glycol chitosan conjugates. (United States)

    Denora, Nunzio; Lopedota, Angela; Perrone, Mara; Laquintana, Valentino; Iacobazzi, Rosa M; Milella, Antonella; Fanizza, Elisabetta; Depalo, Nicoletta; Cutrignelli, Annalisa; Lopalco, Antonio; Franco, Massimo


    This work describes N-acetylcysteine (NAC)- and glutathione (GSH)-glycol chitosan (GC) polymer conjugates engineered as potential platform useful to formulate micro-(MP) and nano-(NP) particles via spray-drying techniques. These conjugates are mucoadhesive over the range of urine pH, 5.0-7.0, which makes them advantageous for intravesical drug delivery and treatment of local bladder diseases. NAC- and GSH-GC conjugates were generated with a synthetic approach optimizing reaction times and purification in order to minimize the oxidation of thiol groups. In this way, the resulting amount of free thiol groups immobilized per gram of NAC- and GSH-GC conjugates was 6.3 and 3.6mmol, respectively. These polymers were completely characterized by molecular weight, surface sulfur content, solubility at different pH values, substitution and swelling degree. Mucoadhesion properties were evaluated in artificial urine by turbidimetric and zeta (ζ)-potential measurements demonstrating good mucoadhesion properties, in particular for NAC-GC at pH 5.0. Starting from the thiolated polymers, MP and NP were prepared using both the Büchi B-191 and Nano Büchi B-90 spray dryers, respectively. The resulting two formulations were evaluated for yield, size, oxidation of thiol groups and ex-vivo mucoadhesion. The new spray drying technique provided NP of suitable size (polymers, avoiding thiolic oxidation during the formulation. MP with acceptable size produced by spray-dryer Büchi B-191 were compared with NP made with the apparatus Nano Büchi B-90. Copyright © 2016 Acta Materialia Inc. All rights reserved.

  16. Outlining adequate protocols for Lidia bull epididymal storage and sperm cryopreservation: use of glycerol, dimethylformamide and N-acetylcysteine

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    Elvira Matilla


    Full Text Available The Lidia bovine breed is an important hallmark of the Spanish cattle industry. Bulls are selected based upon aggressiveness and epididymal sperm cryopreservation is the way to obtain and store their genetics. There are not specifically designed protocols yet to perform Lidia bull sperm cryopreservation. The present study aimed to determine if a tris-fructose-citrate-egg yolk (20% v/v; TFY extender supplemented with 7% glycerol (TFY1 or 3.5% glycerol plus 3.5% dimethylformamide (DMF; TFY2 are suitable media for cryopreservation of epididymal Lidia bull sperm. Moreover, the effect of N-acetylcysteine (NAC, a potent antioxidant, was evaluated. The epididymis were stored at 4°C for 24, 48, 72 or 96 h, and both freezing media were tested as such or supplemented with 1 or 2.5 mM of NAC. Our data demonstrated that post-thaw viability was well maintained (TFY1: 50.8% ± 1.9 at 24 h and 52.4% ± 0.8 at 96 h and TFY2: 52.6% ± 1.6 at 24 h and 56.1% ± 1.8 at 96 h; mean % ± SEM; p>0.05 as also were total and progressive sperm motility, high mitochondrial membrane potential, ROS production, DNA status and acrosomal intactness of Lidia bull sperm up to 96 h of epididymal storage, all extender variations being similar (p>0.05. In conclusion, the use of TFY medium supplemented either with 7% glycerol alone or the combination of 3.5% glycerol and 3.5% DMF were equally safe choices for epididymal Lidia bull sperm cryopreservation, and NAC addition did not significantly improve sperm post-thaw quality.

  17. Effects of adjunctive N-acetylcysteine on depressive symptoms: Modulation by baseline high-sensitivity C-reactive protein. (United States)

    Porcu, Mauro; Urbano, Mariana Ragassi; Verri, Waldiceu A; Barbosa, Decio Sabbatini; Baracat, Marcela; Vargas, Heber Odebrecht; Machado, Regina Célia Bueno Rezende; Pescim, Rodrigo Rossetto; Nunes, Sandra Odebrecht Vargas


    Outcomes in a RCTs of 12 weeks of theclinical efficacy of N-acetylcysteine (NAC) as an adjunctive treatment on depression and anxiety symptoms and its effects on high-sensitivity C-reactive protein (hs-CRP) levels. A wide array of measures were made. The 17-item version of the Hamilton Depression Rating Scale (HDRS17); the Hamilton Anxiety Rating Scale (HAM-A); Sheehan Disability Scale; Quality of Life; Clinical Global Impression (CGI); anthropometrics measures; and vital signs and biochemical laboratory. There were no significant differences among the groups regarding demographic, clinical features, use of medication, metabolic syndrome and comorbidities. From baseline to week 12, individuals receiving NAC, versus placebo, had a statistically significant reduction in depressive symptoms on HDRS 17 (p  3 mg/L at baseline. Individuals receiving NAC with baseline levels of hs-CRP > 3 mg/L, had more significant reduction in uric acid levels compared to individuals with baseline levels of hs-CRP ≤ 3 mg/L on week 12. Participants receiving placebogained significantly more weight during the 12 weeks for baseline levels of hs-CRP ≤ 3 mg/L and hs-CRP > 3 mg/L, and individuals receiving NAC in both groups did not have significant weight change during the 12 weeks. No individuals were withdrawn from the study because of adverse event. NAC group exhibited significantly greater reduction on hs-CRP levels than placebo group from baseline to week 12. Identifier; NCT02252341. Copyright © 2018 Elsevier B.V. All rights reserved.

  18. A Double-Blind Randomized Controlled Pilot Trial of N-Acetylcysteine in Veterans with PTSD and Substance Use Disorders (United States)

    Back, Sudie E.; McCauley, Jenna L.; Korte, Kristina J.; Gros, Daniel F.; Leavitt, Virginia; Gray, Kevin M.; Hamner, Mark B.; DeSantis, Stacia M.; Malcolm, Robert; Brady, Kathleen T.; Kalivas, Peter W.


    Objective The antioxidant N-Acetylcysteine (NAC) is being increasingly investigated as a therapeutic agent in the treatment of substance use disorders. Preclinical and clinical findings suggest that NAC normalizes extracellular glutamate by restoring the activity of glutamate transporters and antiporters in the nucleus accumbens. This study explored the efficacy of NAC in the treatment of post-traumatic stress disorder (PTSD), which frequently co-occurs with substance use disorders (SUD) and shares impaired prefrontal cortex regulation of basal ganglia circuitry, in particular at glutamate synapses in the nucleus accumbens. Method Veterans with current PTSD and SUD (N=35) were randomly assigned to receive a double-blind, 8-week course of NAC (2400 mg/day) or placebo plus outpatient group cognitive-behavioral therapy for SUD. Primary outcome measures included PTSD symptoms (Clinician Administered PTSD Scale, PTSD Checklist-Military) and craving (Visual Analogue Scale). Depression (Beck Depression Inventory-II) and substance use (Timeline Follow Back, urine drug screens) were also assessed. Results Participants treated with NAC, as compared to placebo, evidenced significant improvements in PTSD symptoms, craving, and depression. Substance use at the start of treatment was low for both the NAC and placebo groups and no significant between-group differences were observed. NAC was well tolerated and retention was high. Conclusions This is the first randomized controlled trial to investigate NAC as a pharmacological treatment for PTSD. The findings show a significant treatment effect on symptoms of PTSD and drug craving, and provide initial support for the use of NAC in combination with cognitive-behavioral therapy among individuals with co-occurring PTSD and SUD. PMID:27736051

  19. N-Acetylcysteine for Nonsuicidal Self-Injurious Behavior in Adolescents: An Open-Label Pilot Study. (United States)

    Cullen, Kathryn R; Klimes-Dougan, Bonnie; Westlund Schreiner, Melinda; Carstedt, Patricia; Marka, Nicholas; Nelson, Katharine; Miller, Michael J; Reigstad, Kristina; Westervelt, Ana; Gunlicks-Stoessel, Meredith; Eberly, Lynn E


    Nonsuicidal self-injury (NSSI) is common in adolescents and young adults, and few evidence-based treatments are available for this significant problem. N-acetylcysteine (NAC) is a widely available nutritional supplement that has been studied in some psychiatric disorders relevant to NSSI including mood and addictive disorders. This pilot study tested the use of NAC as a potential treatment for NSSI in youth. Thirty-five female adolescents and young adults with NSSI aged 13-21 years were enrolled in this study that had an open-label, single-arm study design. All participants were given oral NAC as follows: 600 mg twice daily (weeks 1-2), 1200 mg twice daily (weeks 3-4), and 1800 mg twice daily (weeks 5-8). Patients were seen every 2 weeks throughout the trial, at which time youth reported the frequency of NSSI episodes. Levels of depression, impulsivity, and global psychopathology were measured at baseline and at the end of the trial using the Beck Depression Inventory-II (BDI-II), Barratt Impulsivity Scale, and Symptoms Checklist-90 (SCL-90). About two-thirds of the enrolled female youth completed the trial (24/35). NAC was generally well tolerated in this sample. NAC treatment was associated with a significant decrease in NSSI frequency at visit 6 and visit 8 compared to baseline. We also found that depression scores and global psychopathology scores (but not impulsivity scores) decreased after NAC treatment. Decrease in NSSI was not correlated with decrease in BDI-II or SCL-90 scores, suggesting these might be independent effects. We provide preliminary evidence that NAC may have promise as a potential treatment option for adolescents with NSSI. The current results require follow-up with a randomized, placebo-controlled trial to confirm efficacy.

  20. N-acetylcysteine possesses antidepressant-like activity through reduction of oxidative stress: behavioral and biochemical analyses in rats. (United States)

    Smaga, Irena; Pomierny, Bartosz; Krzyżanowska, Weronika; Pomierny-Chamioło, Lucyna; Miszkiel, Joanna; Niedzielska, Ewa; Ogórka, Agata; Filip, Małgorzata


    The growing body of evidence implicates the significance of oxidative stress in the pathophysiology of depression. The aim of this paper was to examine N-acetylcysteine (NAC) - a putative precursor of the most important tissue antioxidant glutathione - in an animal model of depression and in ex vivo assays to detect oxidative stress parameters. Imipramine (IMI), a classical and clinically-approved antidepressant drug was also under investigation. Male Wistar rats which underwent either bulbectomy (BULB; removal of the olfactory bulbs) or sham surgery (SHAM; olfactory bulbs were left undestroyed) were treated acutely or repeatedly with NAC (50-100mg/kg, ip) or IMI (10mg/kg, ip). Following 10-daily injections with NAC or IMI or their solvents, or 9-daily injections with a corresponding solvent plus acute NAC or acute IMI forced swimming test on day 10, and locomotor activity were performed; immediately after behavioral tests animals were decapitated. Biochemical tests (the total antioxidant capacity - TAC and the superoxide dismutase activity - SOD) were performed on homogenates in several brain structures. In behavioral studies, chronic (but not acute) administration of NAC resulted in a dose-dependent reduction in the immobility time seen only in BULB rats while chronic IMI produced a significant decrease in this parameter in both SHAM and BULB animals. On the other hand, chronic administration of NAC and IMI resulted in a significant increase in cellular antioxidant mechanisms (SOD activity) that reversed the effects of BULB in the frontal cortex, hippocampus and striatum. Our study further supports the antidepressant-like activity of NAC and links its effect as well as IMI actions with the enhancement of brain SOD activity. Copyright © 2012 Elsevier Inc. All rights reserved.

  1. Effects of N-acetylcysteine and imipramine in a model of acute rhythm disruption in BALB/c mice. (United States)

    Pilz, Luísa K; Trojan, Yasmine; Quiles, Caroline L; Benvenutti, Radharani; Melo, Gabriela; Levandovski, Rosa; Hidalgo, Maria Paz L; Elisabetsky, Elaine


    Circadian rhythm disturbances are among the risk factors for depression, but specific animal models are lacking. This study aimed to characterize the effects of acute rhythm disruption in mice and investigate the effects of imipramine and N-acetylcysteine (NAC) on rhythm disruption-induced changes. Mice were exposed to 12:12-hour followed by 10:10-hour light:dark cycles (LD); under the latter, mice were treated with saline, imipramine or NAC. Rhythms of rest/activity and temperature were assessed with actigraphs and iButtons, respectively. Hole-board and social preference tests were performed at the beginning of the experiment and again at the 8th 10:10 LD, when plasma corticosterone and IL-6 levels were also assessed. Actograms showed that the 10:10 LD schedule prevents the entrainment of temperature and activity rhythms for at least 13 cycles. Subsequent light regimen change activity and temperature amplitudes showed similar patterns of decline followed by recovery attempts. During the 10:10 LD schedule, activity and temperature amplitudes were significantly decreased (paired t test), an effect exacerbated by imipramine (ANOVA/SNK). The 10:10 LD schedule increased anxiety (paired t test), an effect prevented by NAC (30 mg/kg). This study identified mild but significant behavioral changes at specific time points after light regimen change. We suggest that if repeated overtime, these subtle changes may contribute to lasting behavioral disturbancess relevant to anxiety and mood disorders. Data suggest that imipramine may contribute to sustained rhythm disturbances, while NAC appears to prevent rhythm disruption-induced anxiety. Associations between sleep/circadian disturbances and the recurrence of depressive episodes underscore the relevance of potential drug-induced maintenance of disturbed rhythms.

  2. Efficacy of N-acetylcysteine to reduce the effects of aflatoxin B1 intoxication in broiler chickens. (United States)

    Valdivia, A G; Martínez, A; Damián, F J; Quezada, T; Ortíz, R; Martínez, C; Llamas, J; Rodríguez, M L; Yamamoto, L; Jaramillo, F; Loarca-Piña, M G; Reyes, J L


    N-acetylcysteine (NAC) has been used safely in humans and in other mammals as an antidote against several toxic and carcinogenic agents, including aflatoxin B1 (AFB1). The aim of this study was to evaluate the capability of dietary supplementation with NAC to ameliorate the effects of subacute intoxication with AFB1 in broiler chickens. One hundred twenty male Hubbard 1-d-old chickens were allocated into one of four dietary treatments: 1) control group without treatment, 2) purified AFB1 added to diet (3 mg/kg of feed) for 21 d, 3) NAC (800 mg/kg BW, daily), or 4) AFB1 plus NAC at the same doses as Groups 2 and 3. Broilers treated with AFB1 plus NAC were shown to be partially protected against deleterious effects on BW (57.8%), daily weight gain (49.1%), feed conversion index (21.4%), plasma and hepatic total protein concentration (45.2, 66.7%), plasma alanine aminotransferase (67.4%), hepatic glutathione-S-transferase (18.8%), and reduced glutathione liver concentration (75.0%). In addition, they showed less intense liver fading, friable texture, and microvesicular steatosis. In the kidney, thickening of glomerular basement membrane was also less severe in NAC+AFB1-treated chickens than in AFB1-treated chickens. Our results suggest that NAC provided protection against negative effects on performance, liver and renal damage, and biochemical alterations induced by AFB1 in broiler chickens. Effects of NAC alone on chick performance were also evaluated. Addition of NAC to diet (800 mg/kg BW) did not negatively affect feed consumption, conversion index, or serum chemistry and did not induce structural changes in the liver or kidney.

  3. N-acetylcysteine-pretreated human embryonic mesenchymal stem cell administration protects against bleomycin-induced lung injury. (United States)

    Wang, Qiao; Zhu, Hong; Zhou, Wu-Gang; Guo, Xiao-Can; Wu, Min-Juan; Xu, Zhen-Yu; Jiang, Jun-feng; Shen, Ce; Liu, Hou-Qi


    The transplantation of mesenchymal stem cells (MSCs) has been reported to be a promising approach in the treatment of acute lung injury. However, the poor efficacy of transplanted MSCs is one of the serious handicaps in the progress of MSC-based therapy. Therefore, the purpose of this study was to investigate whether the pretreatment of human embryonic MSCs (hMSCs) with an antioxidant, namely N-acetylcysteine (NAC), can improve the efficacy of hMSC transplantation in lung injury. In vitro, the antioxidant capacity of NAC-pretreated hMSCs was assessed using intracellular reactive oxygen species (ROS) and glutathione assays and cell adhesion and spreading assays. In vivo, the therapeutic potential of NAC-pretreated hMSCs was assessed in a bleomycin-induced model of lung injury in nude mice. The pretreatment of hMSCs with NAC improved antioxidant capacity to defend against redox imbalances through the elimination of cellular ROS, increasing cellular glutathione levels, and the enhancement of cell adhesion and spreading when exposed to oxidative stresses in vitro. In addition, the administration of NAC-pretreated hMSCs to nude mice with bleomycin-induced lung injury decreased the pathological grade of lung inflammation and fibrosis, hydroxyproline content and numbers of neutrophils and inflammatory cytokines in bronchoalveolar lavage fluid and apoptotic cells, while enhancing the retention and proliferation of hMSCs in injured lung tissue and improving the survival rate of mice compared with results from untreated hMSCs. The pretreatment of hMSCs with NAC could be a promising therapeutic approach to improving cell transplantation and, therefore, the treatment of lung injury.

  4. Effect of inhaled N-acetylcysteine monotherapy on lung function and redox balance in idiopathic pulmonary fibrosis. (United States)

    Muramatsu, Yoko; Sugino, Keishi; Ishida, Fumiaki; Tatebe, Junko; Morita, Toshisuke; Homma, Sakae


    An oxidant-antioxidant imbalance is considered to be involved in the pathogenesis of idiopathic pulmonary fibrosis (IPF). Therefore, administration of antioxidants, such as N-acetylcysteine (NAC), may represent a potential treatment option for IPF patients. The aim of this study was to evaluate the effect of inhaled NAC monotherapy on lung function and redox balance in patients with IPF. A retrospective observational study was done, involving 22 patients with untreated early IPF (19 men; mean [±S.D.] age, 71.8 [±6.3]y). At baseline and at 6 and 12 months after initiating inhaled NAC monotherapy, we assessed forced vital capacity (FVC) and measured the levels of total glutathione, oxidized glutathione (GSSG), and the ratio of reduced to oxidized glutathione in whole blood (hereafter referred to as the ratio), and of 8-hydroxy-2'-deoxyguanosine in urine. To evaluate response to treatment, we defined disease progression as a decrease in FVC of ≥5% from baseline and stable disease as a decrease in FVC of <5%, over a period of 6 months. Change in FVC in the stable group at 6 and 12 months were 95±170mL and -70±120mL, while those in the progressive group at 6 and 12 months were -210±80mL, -320±350mL, respectively. The serial mean change in GSSG from baseline decreased as the ratio of reduced to oxidized glutathione increased in patients with stable disease, while it increased as this ratio decreased in patients with progressive disease. Receiver operating characteristic curve analysis revealed that a baseline GSSG level of ≥1.579μM was optimal for identifying treatment responders. Inhaled NAC monotherapy was associated with improved redox imbalance in patients with early IPF. Copyright © 2015 The Japanese Respiratory Society. Published by Elsevier B.V. All rights reserved.

  5. N-acetylcysteine protects against cadmium-induced germ cell apoptosis by inhibiting endoplasmic reticulum stress in testes. (United States)

    Ji, Yan-Li; Wang, Hua; Zhang, Cheng; Zhang, Ying; Zhao, Mei; Chen, Yuan-Hua; Xu, De-Xiang


    Cadmium (Cd) is a reproductive toxicant that induces germ cell apoptosis in the testes. Previous studies have demonstrated that endoplasmic reticulum (ER) stress is involved in Cd-induced germ cell apoptosis. The aim of the present study was to investigate the effects of N-acetylcysteine (NAC), an antioxidant, on Cd-induced ER stress and germ cell apoptosis in the testes. Male CD-1 mice were intraperitoneally injected with CdCl2 (2.0 mg kg(-1)). As expected, acute Cd exposure induced germ cell apoptosis in the testes, as determined by terminal dUTP nick-end labelling (TUNEL). However, the administration of NAC alleviated Cd-induced germ cell apoptosis in the testes. Further analysis showed that NAC attenuated the Cd-induced upregulation of testicular glucose-regulated protein 78 (GRP78), an important ER molecular chaperone. Moreover, NAC inhibited the Cd-induced phosphorylation of testicular eukaryotic translation initiation factor 2α (eIF2α), a downstream target of the double-stranded RNA-activated kinase-like ER kinase (PERK) pathway. In addition, NAC blocked the Cd-induced activation of testicular X binding protein (XBP)-1, indicating that NAC attenuates the Cd-induced ER stress and the unfolded protein response (UPR). Interestingly, NAC almost completely prevented the Cd-induced elevation of C/EBP homologous protein (CHOP) and phosphorylation of c-Jun N-terminal kinase (JNK), two components of the ER stress-mediated apoptotic pathway. In conclusion, NAC protects against Cd-induced germ cell apoptosis by inhibiting endoplasmic reticulum stress in the testes.

  6. Effects of N-acetylcysteine and terbutaline treatment on hemodynamics and regional albumin extravasation in porcine septic shock

    International Nuclear Information System (INIS)

    Groeneveld, A.B.; den Hollander, W.; Straub, J.; Nauta, J.J.; Thijs, L.G.


    We studied the therapeutic effects of continuously infused N-acetylcysteine, an O2 radical scavenger (N, n = 6), and terbutaline, a beta 2-agonist (T, n = 6), versus dextrose (controls C, N = 6) on hemodynamics and regional albumin extravasation in porcine septic shock. After instrumentation, injection of 99mTc-labeled red blood cells, and baseline measurements, pigs received a 90 min infusion of 11 +/- 9 X 10(8).kg-1 live Escherichia coli bacteria. Thereafter, therapy was started, and 131I human serum albumin was injected. Images were obtained hourly using a gamma camera and a computer until 5 hours after baseline. Regions of interest were drawn in the 99mTc images, yielding regional 131I/99mTc radioactivity ratios, with blood samples as reference. From these ratios, an albumin leak index, a rate constant of transvascular albumin transport, was calculated. Control pigs developed pulmonary hypertension, arterial hypotension, hemoconcentration, and lactic acidemia. In spite of tachycardia and unchanged filling pressures, cardiac output fell. In arterial blood, white cell count, PO2, albumin level, and colloid osmotic pressure fell. The albumin leak index (X10(-3).min-1) measured 1.56 +/- 0.59 over the lungs and 2.87 +/- 1.19 over the abdomen in C, confirming previously found increased albumin flux in both lung and abdomen, the latter exceeding the former. Neither N nor T significantly affected hemodynamic and biochemical changes. The drugs neither decreased the regional albumin leak index nor attenuated the formation of albumin-rich ascites found at autopsy. However, the lung albumin index obtained at autopsy was significantly reduced with N (P less than .01 vs. C), at similar gravimetrically determined extravascular lung water (EVLW). EVLW positively correlated with pulmonary albumin extravasation in C and T but not in N

  7. N-Acetylcysteine and Desferoxamine Reduce Pulmonary Oxidative Stress Caused by Hemorrhagic Shock in a Porcine Model. (United States)

    Mani, Alexandra; Staikou, Chryssoula; Karmaniolou, Iosifina; Orfanos, Nikolaos; Mylonas, Anastassios; Nomikos, Tzortzis; Pafiti, Agathi; Papalois, Apostolos; Arkadopoulos, Nikolaos; Smyrniotis, Vassilios; Theodoraki, Kassiani


    To investigate the pulmonary oxidative stress and possible protective effect of N-Acetylcysteine (NAC) and Desferoxamine (DFX)in a porcine model subjected to hemorrhagic shock. Twenty-one pigs were randomly allocated to Group-A (sham, n = 5), Group-B (fluid resuscitation, n = 8) and Group-C (fluid, NAC and DFX resuscitation, n = 8). Groups B and C were subjected to a 40-min shock period induced by liver trauma, followed by a 60-min resuscitation period. During shock, the mean arterial pressure (MAP) was maintained at 30-40 mmHg. Resuscitation consisted of crystalloids (35 mL/kg) and colloids (18 mL/kg) targeting to MAP normalization (baseline values ± 10%). In addition, Group-C received pretreatment with NAC 200 mg/kg plus DFX 2 g as intravenous infusions. Thiobarbituric Acid Reactive Substances (TBARS), protein carbonyls and glutathione peroxidase (GPx) activity were determined in lung tissue homogenates. Also, histological examination of pulmonary tissue specimens was performed. TBARS were higher in Group-B than in Group-A or Group-C: 2.90 ± 0.47, 0.57 ± 0.10, 1.78 ± 0.47 pmol/μg protein, respectively (p 0.05). GPx activity did not differ significantly between the three groups (p > 0.05). Lung histology was improved in Group-C versus Group-B, with less alveolar collapse, interstitial edema and inflammation. NAC plus DFX prevented the increase of pulmonary oxidative stress markers and protein damage after resuscitated hemorrhagic shock and had beneficial effect on lung histology. NAC/DFX combination may be used in the multimodal treatment of hemorrhagic shock, since it may significantly prevent free radical injury in the lung.

  8. Arterial morphology responds differently to Captopril then N-acetylcysteine in a monocrotaline rat model of pulmonary hypertension (United States)

    Molthen, Robert; Wu, Qingping; Baumgardt, Shelley; Kohlhepp, Laura; Shingrani, Rahul; Krenz, Gary


    Pulmonary hypertension (PH) is an incurable condition inevitably resulting in death because of increased right heart workload and eventual failure. PH causes pulmonary vascular remodeling, including muscularization of the arteries, and a reduction in the typically large vascular compliance of the pulmonary circulation. We used a rat model of monocrotaline (MCT) induced PH to evaluated and compared Captopril (an angiotensin converting enzyme inhibitor with antioxidant capacity) and N-acetylcysteine (NAC, a mucolytic with a large antioxidant capacity) as possible treatments. Twenty-eight days after MCT injection, the rats were sacrificed and heart, blood, and lungs were studied to measure indices such as right ventricular hypertrophy (RVH), hematocrit, pulmonary vascular resistance (PVR), vessel morphology and biomechanics. We implemented microfocal X-ray computed tomography to image the pulmonary arterial tree at intravascular pressures of 30, 21, 12, and 6 mmHg and then used automated vessel detection and measurement algorithms to perform morphological analysis and estimate the distensibility of the arterial tree. The vessel detection and measurement algorithms quickly and effectively mapped and measured the vascular trees at each intravascular pressure. Monocrotaline treatment, and the ensuing PH, resulted in a significantly decreased arterial distensibility, increased PVR, and tended to decrease the length of the main pulmonary trunk. In rats with PH induced by monocrotaline, Captopril treatment significantly increased arterial distensibility and decrease PVR. NAC treatment did not result in an improvement, it did not significantly increase distensibility and resulted in further increase in PVR. Interestingly, NAC tended to increase peripheral vascular density. The results suggest that arterial distensibility may be more important than distal collateral pathways in maintaining PVR at normally low values.

  9. Beneficial Effects of N-acetylcysteine and N-mercaptopropionylglycine on Ischemia Reperfusion Injury in the Heart. (United States)

    Bartekova, Monika; Barancik, Miroslav; Ferenczyova, Kristina; Dhalla, Naranjan S


    Ischemia-reperfusion (I/R) injury of the heart as a consequence of myocardial infarction or cardiac surgery represents a serious clinical problem. One of the most prominent mechanisms of I/R injury is the development of oxidative stress in the heart. In this regard, I/R has been shown to enhance the production of reactive oxygen/nitrogen species in the heart which lead to the imbalance between the pro-oxidants and antioxidant capacities of the endogenous radical-scavenging systems. Increasing the antioxidant capacity of the heart by the administration of exogenous antioxidants is considered beneficial for the heart exposed to I/R. N-acetylcysteine (NAC) and Nmercaptopropionylglycine (MPG) are two sulphur containing amino acid substances, which belong to the broad category of exogenous antioxidants that have been tested for their protective potential in cardiac I/R injury. Pretreatment of hearts with both NAC and MPG has demonstrated that these agents attenuate the I/R-induced alterations in sarcolemma, sarcoplasmic reticulum, mitochondria and myofibrils in addition to improving cardiac function. While experimental studies have revealed promising data suggesting beneficial effects of NAC and MPG in cardiac I/R injury, the results of clinical trials are not conclusive because both positive and no effects of these substances have been reported on the post-ischemic recovery of heart following cardiac surgery or myocardial infarction. It is concluded that both NAC and MPG exert beneficial effects in preventing the I/Rinduced injury; however, further studies are needed to establish their effectiveness in reversing the I/R-induced abnormalities in the heart. Copyright© Bentham Science Publishers; For any queries, please email at

  10. Evaluation of the Effect of Nebulized N-Acetylcysteine on Respiratory Secretions in Mechanically Ventilated Patients: Randomized Clinical Trial

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    Seyed Masoom Masoompour


    Full Text Available Background: The purpose of our study was to evaluate an inexpensive and available method to reduce mucous impactions in mechanically ventilated patients. Methods: This randomized clinical trial was conducted on 40 mechanically ventilated patients aged 15-90 years. The patients were randomly allocated into two arms; 20 cases and 20 controls. The cases received N-acetylcysteine via their nebulizers, and the control group received normal saline three times a day for one day. We measured the density of respiratory secretion, plateau and peak airway pressures, and O2 saturation at baseline, 12 and 24 hours later. Results: Although the mean secretion density was significantly lower in the NAC group (F (1, 38=8.61, P=0.006, but a repeated measures ANOVA with a Greenhouse-Geisser correction determined that the effect of NAC on mean secretion density did not differ significantly between time points (F (1, 38=3.08, P=0.087. NAC increased O2 saturation significantly between time points (F (1.92, 73.1=4.6, P=0.014. The plateau airway pressures were relatively stable throughout the study in the normal saline and NAC groups (F (1.95, 37.1=0.67, P=0.513. The peak airway pressure did not change significantly during the study in the normal saline and NAC groups (F (1.52, 56.4=0.91, P=0.384. Conclusion: Considering the limitations of the study, nebulized NAC in mechanically ventilated patients was not effective more than normal saline nebulization in reducing the density of mucous plugs. The peak and plateau airway pressures were relatively stable throughout the study in both groups. Trial Registration Number: IRCT201104276312N1.

  11. Prevention of Contrast-Induced Nephropathy With N-Acetylcysteine or Sodium Bicarbonate in Patients With ST-Segment-Myocardial Infarction

    DEFF Research Database (Denmark)

    Thayssen, Per; Lassen, Jens Flensted; Jensen, Svend Eggert


    (CINSTEMI) trial. Patients were randomly assigned in a 1:1:1:1 ratio to receive hydration with sodium chloride together with 1 of 4 prophylactic regimes (1) N-acetylcysteine (NAC), (2) sodium bicarbonate (NaHCO3) infusion, (3) NAC in combination with NaHCO3, or (4) hydration with sodium chloride infusion...... not reduce the rate of CIN significantly compared with hydration with intravenous sodium chloride infusion alone (20.1% versus 20.1% versus 20.8% versus 26.5%; P=NS). However, an increase in serum creatinine >25% from the baseline value to 30 day was significantly lower in patients treated with combined NAC...

  12. N-acetylcysteine Counteracts Adipose Tissue Macrophage Infiltration and Insulin Resistance Elicited by Advanced Glycated Albumin in Healthy Rats

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    Karolline S. da Silva


    Full Text Available Background: Advanced glycation endproducts elicit inflammation. However, their role in adipocyte macrophage infiltration and in the development of insulin resistance, especially in the absence of the deleterious biochemical pathways that coexist in diabetes mellitus, remains unknown. We investigated the effect of chronic administration of advanced glycated albumin (AGE-albumin in healthy rats, associated or not with N-acetylcysteine (NAC treatment, on insulin sensitivity, adipose tissue transcriptome and macrophage infiltration and polarization.Methods: Male Wistar rats were intraperitoneally injected with control (C or AGE-albumin alone, or, together with NAC in the drinking water. Biochemical parameters, lipid peroxidation, gene expression and protein contents were, respectively, determined by enzymatic techniques, reactive thiobarbituric acid substances, RT-qPCR and immunohistochemistry or immunoblot. Carboxymethyllysine (CML and pyrraline (PYR were determined by LC/mass spectrometry (LC-MS/MS and ELISA.Results: CML and PYR were higher in AGE-albumin as compared to C. Food consumption, body weight, systolic blood pressure, plasma lipids, glucose, hepatic and renal function, adipose tissue relative weight and adipocyte number were similar among groups. In AGE-treated animals, insulin resistance, adipose macrophage infiltration and Col12a1 mRNA were increased with no changes in M1 and M2 phenotypes as compared to C-albumin-treated rats. Total GLUT4 content was reduced by AGE-albumin as compared to C-albumin. NAC improved insulin sensitivity, reduced urine TBARS, adipose macrophage number and Itgam and Mrc mRNA and increased Slc2a4 and Ppara. CD11b, CD206, Ager, Ddost, Cd36, Nfkb1, Il6, Tnf, Adipoq, Retn, Arg, and Il12 expressions were similar among groups.Conclusions: AGE-albumin sensitizes adipose tissue to inflammation due to macrophage infiltration and reduces GLUT4, contributing to insulin resistance in healthy rats. NAC antagonizes AGE

  13. The effects of erdosteine, N-acetylcysteine, and vitamin E on nicotine-induced apoptosis of pulmonary cells

    International Nuclear Information System (INIS)

    Demiralay, Rezan; Guersan, Nesrin; Erdem, Havva


    This study was conducted to investigate the frequency of apoptosis in the pulmonary epithelial cells of rats after intratraperitoneal nicotine injection, in order to examine the role of inflammatory markers [myeloperoxidase (MPO) and tumor necrosis factor-alpha (TNF-α)] in nicotine-induced lung damage, and to determine the protective effects of three known antioxidant agents [N-acetylcysteine (NAC), erdosteine, and vitamin E] on the lung toxicity of nicotine in the lungs. Female Wistar rats were divided into seven groups, each composed of nine rats: two negative control groups, two positive control groups, one erdosteine-treated group (500 mg/kg), one NAC-treated group (500 mg/kg), and one vitamin E-treated group (500 mg/kg). Nicotine was injected intraperitoneally at a dosage of 0.6 mg/kg for 21 days. Following nicotine injection, the antioxidants were administered orally, treatment was continued until the rats were killed. Lung tissue samples were stained with hematoxylin-eosin (H and E) for histopathological assessments. The apoptosis level in the lung bronchiolar and alveolar epithelium was determined by using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) method. Cytoplasmic TNF-α in the bronchiolar and alveolar epithelial cells and the lung MPO activity were evaluated immunohistochemically. The protective effect of vitamin E on lung histology was stronger than that of erdosteine or NAC. Treatment with erdosteine, NAC, and vitamin E significantly reduced the rate of nicotine-induced pulmonary epithelial cell apoptosis, and there were no significant differences in apoptosis among the three antioxidants groups. Erdosteine, NAC, and vitamin E significantly reduced the increases in TNF-α staining and lung MPO activity. The effects of erdosteine on the increases in the local TNF-α level and lung MPO activity were weaker than that of NAC or vitamin E. This findings suggest that erdosteine and NAC can be as effective as vitamin

  14. N-acetylcysteine prevents spatial memory impairment induced by chronic early postnatal glutaric acid and lipopolysaccharide in rat pups.

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    Fernanda S Rodrigues

    Full Text Available BACKGROUND AND AIMS: Glutaric aciduria type I (GA-I is characterized by accumulation of glutaric acid (GA and neurological symptoms, such as cognitive impairment. Although this disease is related to oxidative stress and inflammation, it is not known whether these processes facilitate the memory impairment. Our objective was to investigate the performance of rat pups chronically injected with GA and lipopolysaccharide (LPS in spatial memory test, antioxidant defenses, cytokines levels, Na+, K+-ATPase activity, and hippocampal volume. We also evaluated the effect of N-acetylcysteine (NAC on theses markers. METHODS: Rat pups were injected with GA (5 umol g of body weight-1, subcutaneously; twice per day; from 5th to 28th day of life, and were supplemented with NAC (150 mg/kg/day; intragastric gavage; for the same period. LPS (2 mg/kg; E.coli 055 B5 or vehicle (saline 0.9% was injected intraperitoneally, once per day, from 25th to 28th day of life. Oxidative stress and inflammatory biomarkers as well as hippocampal volume were assessed. RESULTS: GA caused spatial learning deficit in the Barnes maze and LPS potentiated this effect. GA and LPS increased TNF-α and IL-1β levels. The co-administration of these compounds potentiated the increase of IL-1β levels but not TNF-α levels in the hippocampus. GA and LPS increased TBARS (thiobarbituric acid-reactive substance content, reduced antioxidant defenses and inhibited Na+, K+-ATPase activity. GA and LPS co-administration did not have additive effect on oxidative stress markers and Na+, K+ pump. The hippocampal volume did not change after GA or LPS administration. NAC protected against impairment of spatial learning and increase of cytokines levels. NAC Also protected against inhibition of Na+,K+-ATPase activity and oxidative markers. CONCLUSIONS: These results suggest that inflammatory and oxidative markers may underlie at least in part of the neuropathology of GA-I in this model. Thus, NAC could

  15. Effect of N-acetylcysteine combined with infliximab on toxic epidermal necrolysis. A proof-of-concept study. (United States)

    Paquet, Philippe; Jennes, Serge; Rousseau, Anne Françoise; Libon, Florence; Delvenne, Philippe; Piérard, Gérald E


    The pathophysiology of toxic epidermal necrolysis (TEN) is thought to be related to a drug-induced oxidative stress combined with TNFα overexpression by keratinocytes. None of the current treatments for TEN including systemic corticosteroids, cyclosporine and intravenous administration of immunoglobulins has proven superior over supportive care only. A total of 10 TEN patients were enrolled to be treated at admission in burn units with the antioxidant N-acetylcysteine [NAC, 150mg/kg in a 20-h intravenous (IV) administration], or the combination of the same IV NAC perfusion with the anti-TNFα antibody infliximab (Remicade(®)), administered at a 5mg/kg dosage as a single 2-h IV administration. TEN was confirmed by a skin biopsy taken from a bullous lesion. At entry in the trial and 48h later, the illness auxiliary score (IAS) of clinical severity was determined and the extent in altered skin area (erythema and blisters) was assessed as a relative body area. Skin biopsies of both clinically uninvolved and erythematous areas were collected and immunohistochemistry was performed for assessing the density of inflammatory cells (CD8+ T cells, CD68+ macrophages) and keratinocytes enriched in intracellular calcium (Ca(++)) identified by the Mac387 anti-calprotectin antibody. No unexpected drug-induced adverse event was noticed. After 48h of both treatment modalities, improvements were not observed in the extent of skin involvement and in IAS. Immunohistopathology showed the absence of reduction in the amount of intraepidermal inflammatory cells. An increased intracellular Ca(++) load in clinically uninvolved keratinocytes and in erythematous epidermis was noticed. This latter finding suggested the progression in the way of the apoptotic process. On burn unit discharge, the survival in each modality of treatment was not improved compared to the expected outcomes determined from the IAS at admission. In this proof-to-concept attempt, NAC treatment or its combination with

  16. N-acetylcysteine prevents HIV gp 120-related damage of human cultured astrocytes: correlation with glutamine synthase dysfunction

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    Costa Nicola


    Full Text Available Abstract Background HIV envelope gp 120 glycoprotein is released during active HIV infection of brain macrophages thereby generating inflammation and oxidative stress which contribute to the development of the AIDS-Dementia Complex (ADC. Gp120 has also been found capable to generate excitotoxic effect on brain tissue via enhancement of glutamatergic neurotransmission, leading to neuronal and astroglial damage, though the mechanism is still to be better understood. Here we investigated on the effect of N-acetylcysteine (NAC, on gp120-induced damage in human cultured astroglial cells and the possible contribution of gp120-related reacting oxygen species (ROS in the imbalanced activity of glutamine synthase (GS, the enzyme that metabolizes glutamate into glutamine within astroglial cells playing a neuroprotective role in brain disorders. Results Incubation of Lipari human cultured astroglial cells with gp 120 (0.1–10 nM produced a significant reduction of astroglial cell viability and apoptosis as evaluated by TUNEL reaction and flow cytometric analysis (FACS. This effect was accompanied by lipid peroxidation as detected by means of malondialdehyde assay (MDA. In addition, gp 120 reduced both glutamine concentration in astroglial cell supernatants and GS expression as detected by immunocytochemistry and western blotting analysis. Pre-treatment of cells with NAC (0.5–5 mM, dose-dependently antagonised astroglial apoptotic cell death induced by gp 120, an effect accompanied by significant attenuation of MDA accumulation. Furthermore, both effects were closely associated with a significant recovery of glutamine levels in cell supernatants and by GS expression, thus suggesting that overproduction of free radicals might contribute in gp 120-related dysfunction of GS in astroglial cells. Conclusion In conclusion, the present experiments demonstrate that gp 120 is toxic to astroglial cells, an effect accompanied by lipid peroxidation and by altered

  17. The Levels Of Cytokines And S 100β Which Associated With The Pathogenesis Of Hepatic Encephalopathy In Rats: Role Of Lactulose And N-Acetylcysteine In Its Treatment

    International Nuclear Information System (INIS)

    Heibashy, M.I.A.; Mazen, G.M.A.; Ibrahim, M.A.


    Hepatic encephalopathy (HE) is a reversible syndrome of impaired brain function occurring in patients with advanced liver failure. Recently, it has been reported that pro inflammatory cytokines are involved in the pathogenesis of brain oedema during HE. This study was conducted to elucidate the changes of plasma and brain pro inflammatory cytokines in encephalopathy rats and to evaluate the hepato-protective activity of lactulose or N-acetylcysteine against thioacetamide (TAA) induced hepatopathy in rats. Acute hepatic encephalopathy (HE) in rats was induced by intraperitoneal injection of thioacetamide in 24 hours intervals for two consecutive days. The obtained results revealed significant increase (P<0.05) in liver function tests (ALT, AST, ALP, bilirubin), ammonia, nitric oxide and total oxidant capacity (TOC) in TAA rats than those in control ones. Brain water content was significantly elevated in TAA rats as compared with the control. In addition, the levels of pro inflammatory cytokines (IL-1Β, IL-6, TNF-α) in both serum and brain were significantly increased associated with a remarkable elevation in the level of serum S 100β in TAA rats. On the other hand, induction of hepatic encephalopathy caused significant decrease (P<0.05) in total antioxidant capacity (TAC) levels. When HE rats group was treated with lactulose or N-acetylcysteine, considerable amelioration effects in all previous studied parameters were pronounced dependent on certain mechanisms

  18. Betoninstandsetzung unter Wasser : Mörtelentwicklung und Applikationstechniken


    Nguyen-Sauerbaum, Lina Phan Anh


    Für die Instandsetzung von Betonbauteilen unter Wasser stehen aktuell keine gängigen Verfahren zur Verfügung, die ohne Trockenlegung oder Einschalen des Bauteils auskommen. Diese Arbeit legt die Grundlagen für eine Betoninstandsetzung unter Wasser ohne Einschalen des Bauwerks. Es wurden neue Mörtel entwickelt, die unter Wasser einsetzbar sind und Applikationstechniken untersucht, die eine Applikation unter Wasser mit erfolgreichem Haftverbund erlauben. There are currently no established...

  19. The influence of single application of paracetamol and/or N-acetylcysteine on rats subchronic exposed to trichloroethylene vapours. I. Effect on hepatic moonooxygenase system dependent of cytochrome P450

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    Andrzej Plewka


    Full Text Available Background: There is a number of factors which potentially affect occurrence of toxic change in liver after overdosing of paracetamol. Hepatic metabolism of trichloroethylene has primary impact on hepatotoxic effect of this solvent. This means that the combined exposure to these xenobiotics can be particularly harmful for human. The influence of N-acetylcysteine (NAC as a protective factor after paracetamol intoxication was studies. Materials and method: Tests were carried out on rats which were treated with trichloroethylene, paracetamol and/or N-acetylcysteine. In the hepatic microsomal fraction activity of the components of cytochrome P450- dependent monooxygenases was determined Results: Paracetamol slightly stimulated cytochrome P450 having no effect on reductase activity cooperating with it. Cytochrome b5 and its reductase were inhibited by this compound. Trichloroethylene was the inhibitor of compounds of II microsomal electron transport chain. N-acetylcysteine inhibited activity of reductase of NADH-cytochrome b5. Conclusions: Tested doses of the xenobiotics influenced on II microsomal electron transport chain. Protective influence of N-acetylcysteine was better if this compound was applied 2 hours after exposure on xenobiotics

  20. Effect of N-acetylcysteine administration on the expression and activities of antioxidant enzymes and the malondialdehyde level in the blood of lead-exposed workers. (United States)

    Kasperczyk, Sławomir; Dobrakowski, Michał; Kasperczyk, Aleksandra; Machnik, Grzegorz; Birkner, Ewa


    We investigated whether treatment with N-acetylcysteine (NAC) reduces oxidative stress intensity and restores the expression and activities of superoxide dismutase (Sod1, SOD), catalase (Cat, CAT) and glutathione peroxidase (Gpx1, GPx) in lead-exposed workers. The exposed population was divided randomly into two groups. Workers in the first group (reference group, n=49) were not administered any drugs, while workers in the second group (n=122) were treated with NAC at three doses for 12 weeks (200 mg, 400 mg, 800 mg/day). NAC administered orally to lead-exposed workers normalized antioxidant enzyme activities in blood cells. Oxidative stress intensity measured as malondialdehyde (MDA) levels in serum, leukocytes and erythrocytes significantly decreased after NAC administration. NAC may be an alternative therapy for chronic lead intoxication. Copyright © 2014 Elsevier B.V. All rights reserved.

  1. Effect Of N-Acetylcysteine On Biochemical And Gene Expression Changes In Guinea Pig Exposed To GAMMA Radiation And Cigarette Smoke

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    The environmental or silent smoke of tobacco contains a large number of components, and many of them are toxic to the epithelial cells. The environmental smoke contains reactive oxygen species (ROS) and reactive nitrogen species (RNS) that are responsible for 50% of the global mortality, and also 56% of the disease burdens are attributed to tobacco in developing countries. The aim of the present study is to evaluate the effects of ROS and RNS on antioxidant enzymes and expression of eNOS and iNOS genes that synthesis NO in addition to the gene expression of MUC5AC that synthesis mucin. Moreover, the present study aimed also to evaluate the role of N-acetylcysteine (NAC) as antioxidant. Male guinea pigs exposed to cigarette smoke and/or gamma radiation were treated with N-acetylcysteine (NAC). The study included determination of the activities of Cu-Zn superoxide dismutase, Mn-superoxide dismutase, glutathione peroxidase in lung and heart and expressions of eNOS, iNOS and MUC5AC genes in lung tissue. The results revealed significant increase in Mn-superoxide dismutase, iNOS gene expression and MUC5AC gene expression, and significant decrease in eNOS gene expression in lung of guinea pig exposed to cigarette smoke and/or gamma radiation. The results also revealed that NAC can reduce the effects of cigarette smoke and radiation on antioxidant enzymes and the expression of genes that synthesis NO and MUC5AC that synthesis mucin. It could be concluded that NAC can ameliorate the action of the bad effects of cigarette smoke and gamma radiation.

  2. In search of better spermatogonial preservation by supplementation of cryopreserved human immature testicular tissue xenografts with N-acetylcysteine and testosterone

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    Jonathan ePoels


    Full Text Available Controlled slow-freezing is the procedure currently applied for immature testicular tissue cryobanking in clinical practice. Vitrification has been proposed as a promising alternative, with a view to better preserving spermatogonial stem cells for future fertility restoration by autografting in young boys suffering from cancer. It appears that besides the potential influence of the cryopreservation technique used, the transplantation procedure itself has a significant impact on spermatogonial loss observed in ITT xenografts. Eighteen immature testicular tissue pieces issuing from 6 patients aged 2-15 years were used. Fragments of fresh tissue (serving as ungrafted controls, frozen-thawed tissue, frozen-thawed tissue supplemented with N-acetylcysteine and frozen-thawed tissue supplemented with testosterone xenografted to nude mice for 5 days were compared. Upon graft removal, histological and immunohistochemical analyses were performed to evaluate spermatogonia, intratubular proliferation and intrinsic and extrinsic apoptosis. A significant decrease in the integrity of intact seminiferous tubules was found in all three grafted groups. Spermatogonia were observed by immunohistochemistry in all grafted groups, with recovery rates of 67%, 63% and 53% respectively for slow-frozen tissue, slow-frozen tissue supplemented with N-acetylcysteine and slow-frozen tissue supplemented with testosterone. Apoptosis evidenced by active caspase-3 and TUNEL was similar in all grafts. The study is limited by the low availability of immature testicular tissue samples of human origin, and no clear impact of graft supplementation was found. The mouse xenotransplantation model needs to be refined to investigate human spermatogenesis in human immature testicular tissue grafts.

  3. The effect of adjuvant N-acetylcysteine effervescent tablets therapy on cardiopulmonary function and airway remodeling in patients with stable COPD

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    Gui-Fang Hu1


    Full Text Available Objective: To study the effect of adjuvant N-acetylcysteine (NAC effervescent tablets therapy on cardiopulmonary function and airway remodeling in patients with stable chronic obstructive pulmonary disease (COPD. Methods: Patients with stable COPD treated in Zigong Third People’s Hospital and West China Hospital, Sichuan University between May 2014 and October 2016 were selected and randomly divided into two groups, NAC group received N-acetylcysteine effervescent tablets combined with routine treatment, and control group received routine treatment. Before treatment as well as 2 weeks and 4 weeks after treatment, oxidative stress indexes and airway remodeling indexes in serum as well as inflammatory response indexes in peripheral blood were determined. Results: MDA, PC, 8-OHdG, MMP2, MMP3 and MMP9 contents in serum as well as NLRP3, ASC, p38MAPK and TREM-1 mRNA expression levels in peripheral blood mononuclear cells of both groups of patients after treatment were significantly lower than those before treatment while TAC levels as well as TIMP1 and TIMP2 contents in serum were significantly higher than those before treatment, and MDA, PC, 8-OHdG, MMP2, MMP3 and MMP9 contents in serum a well as NLRP3, ASC, p38MAPK and TREM-1 mRNA expression levels in peripheral blood mononuclear cells of NAC group after treatment were significantly lower than those of control group while TAC levels as well as TIMP1 and TIMP2 contents in serum were significantly higher than those of control group. Conclusion: Adjuvant NAC effervescent tablets treatment of stable COPD can improve the effect of oxidative stress and inflammatory response on cardiopulmonary function, and inhibit the airway remodeling caused by protease activation.

  4. Comparative Effects of Phosphoenolpyruvate, a Glycolytic Intermediate, as an Organ Preservation Agent with Glucose and N-Acetylcysteine against Organ Damage during Cold Storage of Mouse Liver and Kidney


    Ishitsuka, Yoichi; Fukumoto, Yusuke; Kondo, Yuki; Irikura, Mitsuru; Kadowaki, Daisuke; Narita, Yuki; Hirata, Sumio; Moriuchi, Hiroshi; Maruyama, Toru; Hamasaki, Naotaka; Irie, Tetsumi


    We evaluated the usefulness of phosphoenolpyruvate (PEP), a glycolytic intermediate with antioxidative and energy supplementation potentials, as an organ preservation agent. Using ex vivo mouse liver and kidney of a static cold storage model, we compared the effects of PEP against organ damage and oxidative stress during cold preservation with those of glucose or N-acetylcysteine (NAC). Lactate dehydrogenase (LDH) leakage, histological changes, and oxidative stress parameters (measured as thi...

  5. Add-on treatment with N-acetylcysteine for bipolar depression:a 24-week randomized double-blind parallel group placebo-controlled multicentre trial (NACOS-study protocol)


    Ellegaard, Pernille Kempel; Licht, Rasmus Wentzer; Poulsen, Henrik Enghusen; Nielsen, René Ernst; Berk, Michael; Dean, Olivia May; Mohebbi, Mohammadreza; Nielsen, Connie Thuroee


    BACKGROUND: Oxidative stress and inflammation may be involved in the development and progression of mood disorders, including bipolar disorder. Currently, there is a scarcity of useful treatment options for bipolar depressive episodes, especially compared with the efficacy of treatment for acute mania. N-Acetylcysteine (NAC) has been explored for psychiatric disorders for some time given its antioxidant and anti-inflammatory properties. The current trial aims at testing the clinical effects o...

  6. N-acetylcysteine improves redox status, mitochondrial dysfunction, mucin-depleted crypts and epithelial hyperplasia in dextran sulfate sodium-induced oxidative colitis in mice. (United States)

    Amrouche-Mekkioui, Ilhem; Djerdjouri, Bahia


    The effect of N-acetylcysteine (NAC), a pharmacological antioxidant was investigated in a murine model of chronic colitis. Male NMRI mice were given 5% dextran sulfate sodium (DSS) in drinking water for 5 days followed by 10 days of water, three times. Compared to control mice given water, DSS-treated mice displayed severe imbalanced redox status with decreased glutathione and catalase, but increased malondialdehyde, protein carbonyls, nitric oxide and myeloperoxidase levels, at days 35th (active colitis) and 45th (recovery period). It also resulted in mitochondrial dysfunction, mucosal ulcers, mucin-depleted crypts and epithelial cell apoptosis. Crypt abscesses and glandular hyperplasia occurred selectively in distal colon. NAC (150 mg/kg) given in drinking water for 45 days along with 3 DSS cycles improved the hallmarks of DSS-colitis. Interestingly, the moderate impact of NAC on lipids and proteins oxidation correlated with myeloperoxidase and nitric oxide levels.NAC as a mucoregulator and a thiol restoring agent is protective on oxidative crypt alterations, mucin depletion, epithelial cell hyperplasia and apoptosis. Taken together, our results highlight the role of NAC as a scavenger of phagocytes-derived reactive oxygen species in mice DDS-colitis, suggesting that a long term NAC diet might be beneficial in inflammatory bowel diseases and colorectal cancer. Copyright © 2012 Elsevier B.V. All rights reserved.

  7. Inhibition of rotavirus ECwt infection in ICR suckling mice by N-acetylcysteine, peroxisome proliferator-activated receptor gamma agonists and cyclooxygenase-2 inhibitors

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    Carlos Arturo Guerrero


    Full Text Available Live attenuated vaccines have recently been introduced for preventing rotavirus disease in children. However, alternative strategies for prevention and treatment of rotavirus infection are needed mainly in developing countries where low vaccine coverage occurs. In the present work, N-acetylcysteine (NAC, ascorbic acid (AA, some nonsteroidal anti-inflammatory drugs (NSAIDs and peroxisome proliferator-activated receptor gamma (PPARγ agonists were tested for their ability to interfere with rotavirus ECwt infectivity as detected by the percentage of viral antigen-positive cells of small intestinal villi isolated from ECwt-infected ICR mice. Administration of 6 mg NAC/kg every 8 h for three days following the first diarrhoeal episode reduced viral infectivity by about 90%. Administration of AA, ibuprofen, diclofenac, pioglitazone or rosiglitazone decreased viral infectivity by about 55%, 90%, 35%, 32% and 25%, respectively. ECwt infection of mice increased expression of cyclooxygenase-2, ERp57, Hsc70, NF-κB, Hsp70, protein disulphide isomerase (PDI and PPARγ in intestinal villus cells. NAC treatment of ECwt-infected mice reduced Hsc70 and PDI expression to levels similar to those observed in villi from uninfected control mice. The present results suggest that the drugs tested in the present work could be assayed in preventing or treating rotaviral diarrhoea in children and young animals.

  8. Targeting Glia with N-Acetylcysteine Modulates Brain Glutamate and Behaviors Relevant to Neurodevelopmental Disorders in C57BL/6J Mice (United States)

    Durieux, Alice M. S.; Fernandes, Cathy; Murphy, Declan; Labouesse, Marie Anais; Giovanoli, Sandra; Meyer, Urs; Li, Qi; So, Po-Wah; McAlonan, Grainne


    An imbalance between excitatory (E) glutamate and inhibitory (I) GABA transmission may underlie neurodevelopmental conditions such as autism spectrum disorder (ASD) and schizophrenia. This may be direct, through alterations in synaptic genes, but there is increasing evidence for the importance of indirect modulation of E/I balance through glial mechanisms. Here, we used C57BL/6J mice to test the hypothesis that striatal glutamate levels can be shifted by N-acetylcysteine (NAC), which acts at the cystine-glutamate antiporter of glial cells. Striatal glutamate was quantified in vivo using proton magnetic resonance spectroscopy. The effect of NAC on behaviors relevant to ASD was examined in a separate cohort. NAC induced a time-dependent decrease in striatal glutamate, which recapitulated findings of lower striatal glutamate reported in ASD. NAC-treated animals were significantly less active and more anxious in the open field test; and NAC-treated females had significantly impaired prepulse inhibition of startle response. This at least partly mimics greater anxiety and impaired sensorimotor gating reported in neurodevelopmental disorders. Thus glial mechanisms regulate glutamate acutely and have functional consequences even in adulthood. Glial cells may be a potential drug target for the development of new therapies for neurodevelopmental disorders across the life-span. PMID:26696857

  9. Flame retardant tris(1,3-dichloro-2-propylphosphate (TDCPP toxicity is attenuated by N-acetylcysteine in human kidney cells

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    David W. Killilea

    Full Text Available Prolonged exposure to the flame retardants found in many household products and building materials is associated with adverse developmental, reproductive, and carcinogenic consequences. While these compounds have been studied in numerous epidemiological and animal models, less is known about the effects of flame retardant exposure on cell function. This study evaluated the toxicity of the commonly used fire retardant tris(1,3-dichloro-2-propylphosphate (TDCPP in cell line derived from the kidney, a major tissue target of organohalogen toxicity. TDCPP inhibited cell growth at lower concentrations (IC50 27 μM, while cell viability and toxicity were affected at higher concentrations (IC50 171 μM and 168 μM, respectively. TDCPP inhibited protein synthesis and caused cell cycle arrest, but only at higher concentrations. Additionally, the antioxidant N-acetylcysteine (NAC reduced cell toxicity in cells treated with TDCPP, suggesting that exposure to TDCPP increased oxidative stress in the cells. In summary, these data show that low concentrations of TDCPP result in cytostasis in a kidney cell line, whereas higher concentrations induce cell toxicity. Furthermore, TDCPP toxicity can be attenuated by NAC, suggesting that antioxidants may be effective countermeasures to some organohalogen exposures. Keywords: flame retardant, cytostasis, cell toxicity, antioxidant, cell cycle

  10. Colonic and Hepatic Modulation by Lipoic Acid and/or N-Acetylcysteine Supplementation in Mild Ulcerative Colitis Induced by Dextran Sodium Sulfate in Rats (United States)

    Moura, Fabiana Andréa; de Andrade, Kívia Queiroz; de Araújo, Orlando Roberto Pimentel; Santos, Juliana Célia de Farias


    Lipoic acid (LA) and N-acetylcysteine (NAC) are antioxidant and anti-inflammatory agents that have not yet been tested on mild ulcerative colitis (UC). This study aims to evaluate the action of LA and/or NAC, on oxidative stress and inflammation markers in colonic and hepatic rat tissues with mild UC, induced by dextran sodium sulfate (DSS) (2% w/v). LA and/or NAC (100 mg·kg·day−1, each) were given, once a day, in the diet, in a pretreatment phase (7 days) and during UC induction (5 days). Colitis induction was confirmed by histological and biochemical analyses (high performance liquid chromatography, spectrophotometry, and Multiplex®). A redox imbalance occurred before an immunological disruption in the colon. NAC led to a decrease in hydrogen peroxide (H2O2), malondialdehyde (MDA) levels, and myeloperoxidase activity. In the liver, DSS did not cause damage but treatments with both antioxidants were potentially harmful, with LA increasing MDA and LA + NAC increasing H2O2, tumor necrosis factor alpha, interferon gamma, and transaminases. In summary, NAC exhibited the highest colonic antioxidant and anti-inflammatory activity, while LA + NAC caused hepatic damage. PMID:27957238

  11. Evaluation of the antioxidant properties of N-acetylcysteine in human platelets: prerequisite for bioconversion to glutathione for antioxidant and antiplatelet activity. (United States)

    Gibson, Kyle R; Neilson, Ilene L; Barrett, Fiona; Winterburn, Tim J; Sharma, Sushma; MacRury, Sandra M; Megson, Ian L


    N-Acetylcysteine (NAC) is a frequently used "antioxidant" in vitro, but the concentrations applied rarely correlate with those encountered with oral dosing in vivo. Here, we investigated the in vitro antioxidant and antiplatelet properties of NAC at concentrations (10-100 microM) that are achievable in plasma with tolerable oral dosing. The impact of NAC pretreatment (2 hours) on aggregation of platelets from healthy volunteers in response to thrombin and adenosine diphosphate and on platelet-derived nitric oxide (NO) was examined. NAC was found to be a weak reducing agent and a poor antioxidant compared with glutathione (reduced form) (GSH). However, platelets treated with NAC showed enhanced antioxidant activity and depression of reactive oxygen species generation associated with increases in intraplatelet GSH levels. An approximately 2-fold increase in NO synthase-derived nitrite was observed with 10 microM NAC treatment, but the effect was not concentration dependent. Finally, NAC significantly reduced both thrombin-induced and adenosine diphosphate-induced platelet aggregation. NAC should be considered a weak antioxidant that requires prior conversion to GSH to convey antioxidant and antithrombotic benefit at therapeutically relevant concentrations. Our results suggest that NAC might be an effective antiplatelet agent in conditions where increased oxidative stress contributes to heightened risk of thrombosis but only if the intraplatelet machinery to convert it to GSH is functional.

  12. N-acetylcysteine fails to modulate the in vitro function of sarcoplasmic reticulum of diaphragm in the final phase of fatigue. (United States)

    Mishima, T; Yamada, T; Matsunaga, S; Wada, M


    In the present study, we tested the hypothesis whether N-acetylcysteine (NAC), a non-specific antioxidant, might influence fatigue by modulating Ca2+-handling capacity by the sarcoplasmic reticulum (SR). In the presence (10 mm) or absence of NAC, bundles of rat diaphragm were stimulated with tetanic trains (350 ms, 30-40 Hz) at 1 train every 2 s for 300 s. SR functions, as assessed by SR Ca2+-uptake and release rates and SR Ca2+-ATPase activity, were measured in vitro on muscle homogenates. Following the 300-s stimulation, the force developed by NAC-treated muscles is approximately 1.8-fold higher (P depression in SR function (P < 0.05). Despite the differing degrees of fatigue between NAC-treated and non-treated muscles, SR functions in these muscles were reduced to similar extents. These results suggest that modulation of SR function measured in vitro may not be a major contributor to inhibition of diaphragmic fatigue with antioxidant, at least, in the final phase of fatigue where force output is remarkably reduced.

  13. Role of reactive oxygen intermediates in the interferon-mediated depression of hepatic drug metabolism and protective effect of N-acetylcysteine in mice. (United States)

    Ghezzi, P; Bianchi, M; Gianera, L; Landolfo, S; Salmona, M


    Interferon (IFN) and IFN inducers are known to depress hepatic microsomal cytochrome P-450 levels, and the liver toxicity of IFN was reported to be lethal in newborn mice. We have observed that administration to mice of IFN and IFN inducers caused a marked increase in liver xanthine oxidase activity. Because this enzyme is well known to produce reactive oxygen intermediates and cytochrome P-450 was reported to be sensitive to the oxidative damage, we have tested the hypothesis that a free radical mechanism could mediate the depression of cytochrome P-450 levels by IFN. Administration to mice of the IFN inducer polyinosinic-polycytidylic acid (2 mg/kg i.p.) caused a 29 to 52% decrease in liver cytochrome P-450. Concomitant p.o. administration of the free radical scavenger, N-acetylcysteine (as a 2.5% solution in drinking water), or the xanthine oxidase inhibitor, allopurinol (100 mg/kg), protected against the IFN-mediated depression of P-450 kg), protected against the IFN-mediated depression of P-450 levels. The results suggest that an increased endogenous generation of free radicals, possibly due to the induction of xanthine oxidase, is implicated in the IFN-mediated depression of liver drug metabolism. The relevance of these data also extends to cases in which this side effect is observed in pathological situations (e.g., viral diseases and administration of vaccines) associated with an induction of IFN.

  14. Synergist effects of n-acetylcysteine and deferoxamine treatment on behavioral and oxidative parameters induced by chronic mild stress in rats. (United States)

    Arent, Camila O; Réus, Gislaine Z; Abelaira, Helena M; Ribeiro, Karine F; Steckert, Amanda V; Mina, Francielle; Dal-Pizzol, Felipe; Quevedo, João


    A growing body of evidence has pointed to a relationship between oxidative stress and depression. Thus, the present study was aimed at evaluating the effects of the antioxidants n-acetylcysteine (NAC), deferoxamine (DFX) or their combination on sweet food consumption and oxidative stress parameters in rats submitted to 40days of exposure to chronic mild stress (CMS). Our results showed that in stressed rats treated with saline, there was a decrease in sweet food intake and treatment with NAC or NAC in combination with DFX reversed this effect. Treatment with NAC and DFX decreased the oxidative damage, which include superoxide and TBARS production in submitochondrial particles, and also thiobarbituric acid reactive substances (TBARS) levels and carbonyl proteins in the prefrontal cortex, amygdala and hippocampus. Treatment with NAC and DFX also increased the activity of the antioxidant enzymes, superoxide dismutase and catalase in the same brain areas. Even so, a combined treatment with NAC and DFX produced a stronger increase of antioxidant activities in the prefrontal cortex, amygdala and hippocampus. The results described here indicate that co-administration may induce a more pronounced antidepressant activity than each treatment alone. In conclusion, these results suggests that treatment with NAC or DFX alone or in combination on oxidative stress parameters could have positive effects against neuronal damage caused by oxidative stress in major depressive disorders. Copyright © 2012 Elsevier Ltd. All rights reserved.

  15. [The effect of prophylactically administered n-acetylcysteine on clinical indicators for tissue oxygenation during hyperoxic ventilation in cardiac risk patients]. (United States)

    Spies, C; Giese, C; Meier-Hellmann, A; Specht, M; Hannemann, L; Schaffartzik, W; Reinhart, K


    Hyperoxic ventilation, used to prevent hypoxia during potential periods of hypoventilation, has been reported to paradoxically decrease whole-body oxygen consumption (VO2). Reduction in nutritive blood flow due to oxygen radical production is one possible mechanism. We investigated whether pretreatment with the sulfhydryl group donor and O2 radical scavenger N-acetylcysteine (NAC) would preserve VO2 and other clinical indicators of tissue oxygenation in cardiac risk patients. Thirty patients, requiring hemodynamic monitoring (radial and pulmonary artery catheters) because of cardiac risk factors, were included in this randomized investigation. All patients exhibited stable clinical conditions (hemodynamics, body temperature, hemoglobin, F1O2 depression ( > 0.2 mV) was significantly less marked in the NAC group (NAC: -0.02 +/- 0.17 vs placebo: -0.23 +/- 0.15; P depression if patients were prophylactically treated with NAC. This suggests that pretreatment with NAC could be considered to attenuate impaired tissue oxygenation and to preserve myocardial performance better in cardiac risk patients during hyperoxia.

  16. Antioxidant N-acetylcysteine restores systemic nitric oxide availability and corrects depressions in arterial blood pressure and heart rate in diabetic rats. (United States)

    Xia, Zhengyuan; Nagareddy, Prabhakara R; Guo, Zhixin; Zhang, Wei; McNeill, John H


    Increased oxidative stress and reduced nitric oxide (NO) bioactivity are key features of diabetes mellitus that eventually result in cardiovascular abnormalities. We assessed whether N-acetylcysteine (NAC), an antioxidant and glutathione precursor, could prevent the hyperglycaemia induced increase in oxidative stress, restore NO availability and prevent depression of arterial blood pressure and heart rate in vivo in experimental diabetes. Control (C) and streptozotocin-induced diabetic (D) rats were treated or not treated with NAC in drinking water for 8 weeks, initiated 1 week after induction of diabetes. At termination, plasma levels of free 15-F2t-isoprostane, a specific marker of oxygen free radical induced lipid peroxidation, was increased while the plasma total antioxidant concentration was decreased in untreated diabetic rats as compared to control rats (P<0.05). This was accompanied by a significant reduction of plasma levels of nitrate and nitrite, stable metabolites of NO, (P<0.05, D vs. C) and a reduced endothelial NO synthase protein expression in the heart and in aortic and mesenteric artery tissues. Systolic, diastolic and mean arterial blood pressures (SBP, DBP and MAP) and heart rate (HR) were reduced in diabetic rats (P<0.05 vs. C) and NAC normalised the changes that occurred in the diabetic rats. The protective effects may be attributable to restoration of NO bioavailability in the circulation.

  17. The validity and internal structure of the Bipolar Depression Rating Scale: data from a clinical trial of N-acetylcysteine as adjunctive therapy in bipolar disorder. (United States)

    Berk, Michael; Dodd, Seetal; Dean, Olivia M; Kohlmann, Kristy; Berk, Lesley; Malhi, Gin S


    Berk M, Dodd S, Dean OM, Kohlmann K, Berk L, Malhi GS. The validity and internal structure of the Bipolar Depression Rating Scale: data from a clinical trial of N-acetylcysteine as adjunctive therapy in bipolar disorder. The phenomenology of unipolar and bipolar disorders differ in a number of ways, such as the presence of mixed states and atypical features. Conventional depression rating instruments are designed to capture the characteristics of unipolar depression and have limitations in capturing the breadth of bipolar disorder. The Bipolar Depression Rating Scale (BDRS) was administered together with the Montgomery Asberg Rating Scale (MADRS) and Young Mania Rating Scale (YMRS) in a double-blind randomised placebo-controlled clinical trial of N-acetyl cysteine for bipolar disorder (N = 75). A factor analysis showed a two-factor solution: depression and mixed symptom clusters. The BDRS has strong internal consistency (Cronbach's alpha = 0.917), the depression cluster showed robust correlation with the MADRS (r = 0.865) and the mixed subscale correlated with the YMRS (r = 0.750). The BDRS has good internal validity and inter-rater reliability and is sensitive to change in the context of a clinical trial.

  18. Synergic effect of Pt-Co nanoparticles and a dopamine derivative in a nanostructured electrochemical sensor for simultaneous determination of N-acetylcysteine, paracetamole and folic acid

    International Nuclear Information System (INIS)

    Karimi-Maleh, Hassan; Hatami, Mehdi; Moradi, Reza; Khalilzadeh, Mohammad A.; Amiri, Sedighe; Sadeghifar, Hasan


    A carbon paste electrode (CPE) was modified with Pt-Co nanoparticles and 2-(3,4-dihydroxyphenethyl)isoindoline-1,3-dione (3,4-DHPID) and then used for determination of N-acetylcysteine (N-AC) in the presence of paracetamole (PC) and folic acid (FA). The Pt-Co nanoparticles were synthesized by the polyol method and characterized by X-ray diffraction, energy dispersive X-ray analysis and transmission electron microscopy. The modified CPE displays good electrocatalytic activity towards the electrooxidation of N-AC in solution of pH 7.0. It was applied to the determination of N-AC in the presence of PC and FA (with well separated signals peaking at 0.2, 0.55 and 0.86 V vs. Ag/AgCl) by using square wave voltammetry. The peak currents are linearly dependent on the concentrations of N-AC, PC and FA in the respective ranges from 0.07 to 500, 1.0 to 850, and 2.0 to 550 μmol·L −1 , with detection limits of 0.009, 0.6 and 0.8 μmol·L −1 . The modified CPE was applied to the determination of N-AC, PC and FA in (spiked) pharmaceutical and biological samples. (author)

  19. Evaluation of Oxidative Stress in Combination Therapy with D-penicillamine and N-Acetylcysteine (NAC in Lead Poisoning in Opium Addicts

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    Saeedeh Shojaeepour


    Full Text Available Background: N-acetylcysteine (NAC is a putative antioxidant and has gained attention as a promising agent for chelating heavy metals including lead. Considering the animal studies results, we hypothesized that adding NAC to the treatment regimen may improve the success of treatment with lead chelators. Methods: A total of 46 patients who were lead-poisoned opioid addicts were divided into two groups randomly and treated with d-penicillamine (DP, 1g/day in four equal divided doses and NAC+DP (1 g/day + 150 mg/kg/day. The efficacy of treatment was evaluated by hospitalization period. Meanwhile, the oxidative stress parameters including lipid peroxidation, protein carbonyl, total antioxidant capacity (TAC, glutathione concentration and super oxide dismutase (SOD activity were determined on admission and discharge and compared with healthy normal controls. Results: Hospitalization period was not different between the two groups. Treatment with DP and DP+NAC significantly decreased oxidative stress in patients. On the discharge day, the SOD activity and TAC were significantly higher in DP+NAC group in comparison with the DP group. Conclusion: Although NAC recovers antioxidant capacity, the advantages of NAC in improvement of DP efficacy in lead poisoning is questionable. Further studies with larger sample size and combination with other chelators are recommended.

  20. N-Acetylcysteine Supplementation Controls Total Antioxidant Capacity, Creatine Kinase, Lactate, and Tumor Necrotic Factor-Alpha against Oxidative Stress Induced by Graded Exercise in Sedentary Men

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    Donrawee Leelarungrayub


    Full Text Available Aim of this study was to evaluate the effects of short-term (7 days N-acetylcysteine (NAC at 1,200 mg daily supplementation on muscle fatigue, maximal oxygen uptake (VO2max, total antioxidant capacity (TAC, lactate, creatine kinase (CK, and tumor necrotic factor-alpha (TNF-α. Twenty-nine sedentary men (13 controls; 16 in the supplement group from a randomized control were included. At before and after supplementation, fatigue index (FI was evaluated in the quadriceps muscle, and performed a graded exercise treadmill test to induce oxidative stress, and as a measure of VO2max. Blood samples were taken before exercise and 20 minutes after it at before and after supplementation, to determine TAC, CK, lactate, and TNF-α levels. Results showed that FI and VO2max increased significantly in the supplement group. After exercise decreased the levels of TAC and increased lactate, CK, and TNF-α of both groups at before supplementation. After supplementation, lactate, CK, and TNF-α levels significantly increased and TAC decreased after exercise in the control group. Whereas the TAC and lactate levels did not change significantly, but CK and TNF-α increased significantly in the supplement group. Therefore, this results showed that NAC improved the muscle fatigue, VO2max, maintained TAC, controlled lactate production, but had no influence on CK and TNF-α.

  1. Targeting glia with N-Acetylcysteine modulates brain glutamate and behaviours relevant to neurodevelopmental disorders in C57BL/6J mice

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    Alice Marie Sybille Durieux


    Full Text Available An imbalance between excitatory (E glutamate and inhibitory (I GABA transmission may underlie neurodevelopmental conditions such as Autism Spectrum Disorder (ASD and schizophrenia. This may be direct, through alterations in synaptic genes, but there is increasing evidence for the importance of indirect modulation of E/I balance through glial mechanisms. Here we used C57BL/6J mice to test the hypothesis that striatal glutamate levels can be shifted by N-acetylcysteine (NAC, which acts at the cystine-glutamate antiporter of glial cells. Striatal glutamate was quantified in-vivo using proton magnetic resonance spectroscopy. The effect of NAC on behaviours relevant to ASD was examined in a separate cohort. NAC induced a time-dependent decrease in striatal glutamate, which recapitulated findings of lower striatal glutamate reported in ASD. NAC-treated animals were significantly less active and more anxious in the open field test; and NAC-treated females had significantly impaired prepulse inhibition of startle response. This at least partly mimics greater anxiety and impaired sensorimotor gating reported in neurodevelopmental disorders. Thus glial mechanisms regulate glutamate acutely and have functional consequences even in adulthood. Glial cells may be a potential drug target for the development of new therapies for neurodevelopmental disorders across the life-span.

  2. The adjuvant effect of metformin and N-acetylcysteine to clomiphene citrate in induction of ovulation in patients with Polycystic Ovary Syndrome. (United States)

    Maged, Ahmed M; Elsawah, Heba; Abdelhafez, Aly; Bakry, Ahmed; Mostafa, Walaa Ai


    To assess the adjuvant effect of metformin and N-acetylcysteine (NAC) to clomiphene citrate (CC) in induction of ovulation in Polycystic Ovary Syndrome (PCOS) patients. 120 women with PCOS were randomly divided into three equal groups: group I received CC only, group II received CC plus NAC and group III received CC plus metformin. There was a significant difference between group II and other two groups regarding average number of ovulatory follicles >18 mm (2.25 versus 1.75 and 1.89, respectively), but no significant difference between the three study groups regarding number of intermediate follicles 14-18 mm (4, 10 and 4, respectively). There was no significant difference between the three study groups regarding occurrence and laterality of ovulation, pregnancy rate per cycle but a significant difference between group II and other two groups regarding pregnancy rate per patient (20% versus 10% and 10%, respectively, p value 0.05). There was a highly statistically significant difference between group II and other two groups regarding peak endometrial thickness (7.3 ± 1.1 versus 5.4 ± 0.6 and 5.3 ± 0.6, respectively). NAC as an adjuvant to CC for induction of ovulation improves ovulation and pregnancy rates in PCOS patients with beneficial impacts on endometrial thickness.

  3. N-Acetylcysteine in Agriculture, a Novel Use for an Old Molecule: Focus on Controlling the Plant–Pathogen Xylella fastidiosa (United States)

    Muranaka, Lígia S.; Giorgiano, Thais E.; Takita, Marco A.; Forim, Moacir R.; Silva, Luis F. C.; Coletta-Filho, Helvécio D.; Machado, Marcos A.; de Souza, Alessandra A.


    Xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it causes Citrus Variegated Chlorosis (CVC). The mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. In this study, we investigated the inhibitory effect of N-Acetylcysteine (NAC), a cysteine analogue used mainly to treat human diseases, on X. fastidiosa in different experimental conditions. Concentrations of NAC over 1 mg/mL reduced bacterial adhesion to glass surfaces, biofilm formation and the amount of exopolysaccharides (EPS). The minimal inhibitory concentration of NAC was 6 mg/mL. NAC was supplied to X. fastidiosa-infected plants in hydroponics, fertigation, and adsorbed to organic fertilizer (NAC-Fertilizer). HPLC analysis indicated that plants absorbed NAC at concentrations of 0.48 and 2.4 mg/mL but not at 6 mg/mL. Sweet orange plants with CVC symptoms treated with NAC (0.48 and 2.4 mg/mL) in hydroponics showed clear symptom remission and reduction in bacterial population, as analyzed by quantitative PCR and bacterial isolation. Experiments using fertigation and NAC-Fertilizer were done to simulate a condition closer to that normally is used in the field. For both, significant symptom remission and a reduced bacterial growth rate were observed. Using NAC-Fertilizer the lag for resurgence of symptoms on leaves after interruption of the treatment increased to around eight months. This is the first report of the anti-bacterial effect of NAC against a phytopathogenic bacterium. The results obtained in this work together with the characteristics of this molecule indicate that the use of NAC in agriculture might be a new and sustainable strategy for controlling plant pathogenic bacteria. PMID:24009716

  4. N-acetylcysteine in agriculture, a novel use for an old molecule: focus on controlling the plant-pathogen Xylella fastidiosa. (United States)

    Muranaka, Lígia S; Giorgiano, Thais E; Takita, Marco A; Forim, Moacir R; Silva, Luis F C; Coletta-Filho, Helvécio D; Machado, Marcos A; de Souza, Alessandra A


    Xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it causes Citrus Variegated Chlorosis (CVC). The mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. In this study, we investigated the inhibitory effect of N-Acetylcysteine (NAC), a cysteine analogue used mainly to treat human diseases, on X. fastidiosa in different experimental conditions. Concentrations of NAC over 1 mg/mL reduced bacterial adhesion to glass surfaces, biofilm formation and the amount of exopolysaccharides (EPS). The minimal inhibitory concentration of NAC was 6 mg/mL. NAC was supplied to X. fastidiosa-infected plants in hydroponics, fertigation, and adsorbed to organic fertilizer (NAC-Fertilizer). HPLC analysis indicated that plants absorbed NAC at concentrations of 0.48 and 2.4 mg/mL but not at 6 mg/mL. Sweet orange plants with CVC symptoms treated with NAC (0.48 and 2.4 mg/mL) in hydroponics showed clear symptom remission and reduction in bacterial population, as analyzed by quantitative PCR and bacterial isolation. Experiments using fertigation and NAC-Fertilizer were done to simulate a condition closer to that normally is used in the field. For both, significant symptom remission and a reduced bacterial growth rate were observed. Using NAC-Fertilizer the lag for resurgence of symptoms on leaves after interruption of the treatment increased to around eight months. This is the first report of the anti-bacterial effect of NAC against a phytopathogenic bacterium. The results obtained in this work together with the characteristics of this molecule indicate that the use of NAC in agriculture might be a new and sustainable strategy for controlling plant pathogenic bacteria.

  5. The role of prophylactic ibuprofen and N-acetylcysteine on the level of cytokines in periapical exudates and the post-treatment pain

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    Ehsani Maryam


    Full Text Available Abstract Background Periapical lesions are inflammatory diseases that result in periapical bone destruction because of host defensive–microbial disturbances. Objective To evaluate the role of prophylactic ibuprofen and N-acetylcysteine (NAC on the levels of tumor necrosis factor alpha (TNF- α, interleukin- 6(IL-6 and IL-17 and post-treatment pain level in chronic periapical lesions. Materials and methods Eighty patients with chronic apical lesions less than 1 cm were randomly assigned to receive NAC tablets (400 mg, ibuprofen tablets (400 mg, NAC (400 mg/ibuprofen (200 mg combination and placebo 90 minutes prior to sampling. Periapical exudates were collected from root canals. TNF- α, IL-6 and IL-17 levels were determined by ELISA and post-treatment pain was assessed using a visual analog scale (VAS. Results There was a significant difference in IL-6 level between ibuprofen group and placebo (p = 0.019. Significant difference in IL-17 level was observed between NAC/ibuprofen combination group and placebo (p = 0.043. Four hours after treatment, a significant difference was observed in VAS pain score between ibuprofen group and placebo (p = 0.017. Eight hours post-treatment, VAS pain score for NAC group was statistically lower than placebo group (p = 0.033. After 12 hours VAS pain score showed a significant decrease in NAC group compared to placebo (p = 0.049. Conclusion The prophylactic ibuprofen and NAC failed to clearly reflect their effect on cytokines levels in exudates of chronic periapical lesions. On the other hand it seems that NAC can be a substitute for ibuprofen in the management of post endodontic pain.

  6. N-acetylcysteine for the prevention of stricture after circumferential endoscopic submucosal dissection of the esophagus: a randomized trial in a porcine model. (United States)

    Barret, Maximilien; Batteux, Frédéric; Beuvon, Frédéric; Mangialavori, Luigi; Chryssostalis, Ariane; Pratico, Carlos; Chaussade, Stanislas; Prat, Frédéric


    Circumferential endoscopic submucosal dissection (CESD) of the esophagus would allow for both the eradication of Barrett's esophagus and its related complications, such as advanced neoplasia. However, such procedures generally induce inflammatory repair resulting in a fibrotic stricture. N-acetylcysteine (NAC) is an antioxidant that has shown some efficacy against pulmonary and hepatic fibrosis. The aim of our study was to evaluate the benefit of NAC in the prevention of esophageal cicatricial stricture after CESD in a swine model. Two groups of six pigs each were subjected to general anesthesia and CESD: after randomization, a first group received an oral NAC treatment regimen of 100 mg/kg/day, initiated one week before the procedure, whereas a second group was followed without any prophylactic treatment. Follow-up endoscopies took place seven, fourteen, twenty-one, and twenty-eight days after CESD. Necropsy, histological assessment of esophageal inflammation, and fibrosis were performed on day 28. The median esophageal lumen diameter on day 21 (main judgment criterion) was 4 mm (range 2 to 5) in group 1 and 3 mm (range 1 to 7) in group 2 (P = 0.95). No significant difference was observed between the two groups regarding clinical evaluation (time before onset of clinically significant esophageal obstruction), number of dilations, esophageal inflammation and fibrosis, or oxidative stress damage on immunohistochemistry. Despite its antioxidant effect, systemic administration of NAC did not show significant benefit on esophageal fibrosis in our animal model of esophageal wound healing within the experimental conditions of this study. Since the administered doses were relatively high, it seems unlikely that NAC might be a valuable option for the prevention of post-endoscopic esophageal stricture.

  7. The Role of Prophylactic Ibuprofen and N-Acetylcysteine on the Level of Cytokines in Periapical Exudates and the Post-Treatment Pain

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    Seyyed Mohsen Aghajanpour Mir


    Full Text Available Background Periapical lesions are inflammatory diseases that result in periapical bone destruction because of host defensive-microbial disturbances. Objective:To evaluate the role of prophylactic ibuprofen and N-acetylcysteine (NAC on the levels of tumor necrosis factor alpha (TNF- alpha, interleukin- 6(IL-6 and IL-17 and post-treatment pain level in chronic periapical lesions. Materials and methods Eighty patients with chronic apical lesions less than 1 cm were randomly assigned to receive NAC tablets (400 mg, ibuprofen tablets (400 mg, NAC (400 mg/ibuprofen (200 mg combination and placebo 90 minutes prior to sampling. Periapical exudates were collected from root canals. TNF- alpha, IL-6 and IL-17 levels were determined by ELISA and posttreatment pain was assessed using a visual analog scale (VAS. Results:There was a significant difference in IL-6 level between ibuprofen group and placebo (p = 0.019. Significant difference in IL-17 level was observed between NAC/ibuprofen combination group and placebo (p = 0.043. Four hours after treatment, a significant difference was observed in VAS pain score between ibuprofen group and placebo (p = 0.017. Eight hours post-treatment, VAS pain score for NAC group was statistically lower than placebo group (p = 0.033. After 12 hours VAS pain score showed a significant decrease in NAC group compared to placebo (p = 0.049. Conclusion:The prophylactic ibuprofen and NAC failed to clearly reflect their effect on cytokines levels in exudates of chronic periapical lesions. On the other hand it seems that NAC can be a substitute for ibuprofen in the management of post endodontic pain

  8. Ameliorative Effects of Dimetylthiourea and N-Acetylcysteine on Nanoparticles Induced Cyto-Genotoxicity in Human Lung Cancer Cells-A549 (United States)

    Srivastava, Ritesh Kumar; Rahman, Qamar; Kashyap, Mahendra Pratap; Lohani, Mohtashim; Pant, Aditya Bhushan


    We study the ameliorative potential of dimetylthiourea (DMTU), an OH• radical trapper and N-acetylcysteine (NAC), a glutathione precursor/H2O2 scavenger against titanium dioxide nanoparticles (TiO2-NPs) and multi-walled carbon nanotubes (MWCNTs) induced cyto-genotoxicity in cultured human lung cancer cells-A549. Cytogenotoxicity was induced by exposing the cells to selected concentrations (10 and 50 µg/ml) of either of TiO2-NPs or MWCNTs for 24 h. Anti-cytogenotoxicity effects of DMTU and NAC were studied in two groups, i.e., treatment of 30 minutes prior to toxic insult (short term exposure), while the other group received DMTU and NAC treatment during nanoparticles exposure, i.e., 24 h (long term exposure). Investigations were carried out for cell viability, generation of reactive oxygen species (ROS), micronuclei (MN), and expression of markers of oxidative stress (HSP27, CYP2E1), genotoxicity (P53) and CYP2E1 dependent n- nitrosodimethylamine-demethylase (NDMA-d) activity. In general, the treatment of both DMTU and NAC was found to be effective significantly against TiO2-NPs and MWCNTs induced cytogenotoxicity in A549 cells. Long-term treatment of DMTU and NAC during toxic insults has shown better prevention than short-term pretreatment. Although, cells responded significantly to both DMTU and NAC, but responses were chemical specific. In part, TiO2-NPs induced toxic responses were mediated through OH• radicals generation and reduction in the antioxidant defense system. While in the case of MWCNTs, adverse effects were primarily due to altering/hampering the enzymatic antioxidant system. Data indicate the applicability of human lung cancer cells-A549 as a pre-screening tool to identify the target specific prophylactic and therapeutic potential of drugs candidate molecules against nanoparticles induced cellular damages. PMID:21980536

  9. Ameliorative effects of dimetylthiourea and N-acetylcysteine on nanoparticles induced cyto-genotoxicity in human lung cancer cells-A549.

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    Ritesh Kumar Srivastava

    Full Text Available We study the ameliorative potential of dimetylthiourea (DMTU, an OH• radical trapper and N-acetylcysteine (NAC, a glutathione precursor/H₂O₂ scavenger against titanium dioxide nanoparticles (TiO₂-NPs and multi-walled carbon nanotubes (MWCNTs induced cyto-genotoxicity in cultured human lung cancer cells-A549. Cytogenotoxicity was induced by exposing the cells to selected concentrations (10 and 50 µg/ml of either of TiO₂-NPs or MWCNTs for 24 h. Anti-cytogenotoxicity effects of DMTU and NAC were studied in two groups, i.e., treatment of 30 minutes prior to toxic insult (short term exposure, while the other group received DMTU and NAC treatment during nanoparticles exposure, i.e., 24 h (long term exposure. Investigations were carried out for cell viability, generation of reactive oxygen species (ROS, micronuclei (MN, and expression of markers of oxidative stress (HSP27, CYP2E1, genotoxicity (P⁵³ and CYP2E1 dependent n- nitrosodimethylamine-demethylase (NDMA-d activity. In general, the treatment of both DMTU and NAC was found to be effective significantly against TiO₂-NPs and MWCNTs induced cytogenotoxicity in A549 cells. Long-term treatment of DMTU and NAC during toxic insults has shown better prevention than short-term pretreatment. Although, cells responded significantly to both DMTU and NAC, but responses were chemical specific. In part, TiO₂-NPs induced toxic responses were mediated through OH• radicals generation and reduction in the antioxidant defense system. While in the case of MWCNTs, adverse effects were primarily due to altering/hampering the enzymatic antioxidant system. Data indicate the applicability of human lung cancer cells-A549 as a pre-screening tool to identify the target specific prophylactic and therapeutic potential of drugs candidate molecules against nanoparticles induced cellular damages.

  10. N-acetylcysteine is able to reduce the oxidation status and the endothelial activation after a high-glucose content meal in patients with Type 2 diabetes mellitus. (United States)

    Masha, A; Brocato, L; Dinatale, S; Mascia, C; Biasi, F; Martina, V


    Post-prandial hyperglycemia seems to play a pivotal role in the pathogenesis of the cardiovascular complications of diabetes mellitus, as it leads to an oxidative stress which in turn causes a reduced NO bioavailability. These conditions produce an endothelial activation. The aim of this study was to assure that the administration of N-acetylcysteine (NAC), thiolic antioxidant, is able to decrease the oxidation status and endothelial activation after a high-glucose content meal. Ten patients with Type 2 diabetes mellitus (DMT2) (Group 1) and 10 normal subjects (Group 2) were studied. They assumed a high-glucose content meal without (phase A) or after (phase B) the administration of NAC. Glycemia, insulinemia, intercellular adhesion molecule 1, vascular adhesion molecule 1 (VCAM-1), E-selectin, malonaldehyde (MDA), and 4-hydroxynonenal (HNE) were assessed at -30, 0, +30, +60, +90, +120, and +180 min with respect to the meal consumption. During the phase A in Group 1, only HNE and MDA levels increased after the meal assumption; all parameters remained unchanged in Group 2. During the phase B, in Group 1, HNE, MDA, VCAM-1, and E-selectin levels after the meal were lower than those in phase A, while no change for all variables were observed in Group 2. A high-glucose meal produces an increase in oxidation parameters in patients with DMT2. The administration of NAC reduces the oxidative stress and, by doing so, reduces the endothelial activation. In conclusion, NAC could be efficacious in the slackening of the progression of vascular damage in DMT2.

  11. Long-term N-acetylcysteine and L-arginine administration reduces endothelial activation and systolic blood pressure in hypertensive patients with type 2 diabetes. (United States)

    Martina, Valentino; Masha, Andi; Gigliardi, Valentina Ramella; Brocato, Loredana; Manzato, Enzo; Berchio, Arrigo; Massarenti, Paola; Settanni, Fabio; Della Casa, Lara; Bergamini, Stefania; Iannone, Anna


    Reactive oxygen and nitric oxide (NO) have recently been considered to be involved in the cardiovascular complications of patients with type 2 diabetes, as NO is thought to lose its beneficial physiological effects in the presence of oxygen radicals. For this reason, we tested the effects of l-arginine (ARG) and N-acetylcysteine (NAC) administration in increasing NO bioavailability by reducing free radical formation. A double-blind study was performed on 24 male patients with type 2 diabetes and hypertension divided into two groups of 12 patients that randomly received either an oral supplementation of placebo or NAC + ARG for 6 months. The NAC + ARG treatment caused a reduction of both systolic (P < 0.05) and diastolic (P < 0.05) mean arterial blood pressure, total cholesterol (P < 0.01), LDL cholesterol (P < 0.005), oxidized LDL (P < 0.05), high-sensitive C-reactive protein (P < 0.05), intracellular adhesion molecule (P < 0.05), vascular cell adhesion molecule (P < 0.01), nitrotyrosine (P < 0.01), fibrinogen (P < 0.01), and plasminogen activator inhibitor-1 (P < 0.05), and an improvement of the intima-media thickness during endothelial postischemic vasodilation (P < 0.02). HDL cholesterol increased (P < 0.05). No changes in other parameters studied were observed. NAC + ARG administration seems to be a potential well-tolerated antiatherogenic therapy because it improves endothelial function in hypertensive patients with type 2 diabetes by improving NO bioavailability via reduction of oxidative stress and increase of NO production. Our study's results give prominence to its potential use in primary and secondary cardiovascular prevention in these patients.

  12. Potentiation of lead-induced cell death in PC12 cells by glutamate: Protection by N-acetylcysteine amide (NACA), a novel thiol antioxidant

    International Nuclear Information System (INIS)

    Penugonda, Suman; Mare, Suneetha; Lutz, P.; Banks, William A.; Ercal, Nuran


    Oxidative stress has been implicated as an important factor in many neurological diseases. Oxidative toxicity in a number of these conditions is induced by excessive glutamate release and subsequent glutamatergic neuronal stimulation. This, in turn, causes increased generation of reactive oxygen species (ROS), oxidative stress, excitotoxicity, and neuronal damage. Recent studies indicate that the glutamatergic neurotransmitter system is involved in lead-induced neurotoxicity. Therefore, this study aimed to (1) investigate the potential effects of glutamate on lead-induced PC12 cell death and (2) elucidate whether the novel thiol antioxidant N-acetylcysteine amide (NACA) had any protective abilities against such cytotoxicity. Our results suggest that glutamate (1 mM) potentiates lead-induced cytotoxicity by increased generation of ROS, decreased proliferation (MTS), decreased glutathione (GSH) levels, and depletion of cellular adenosine-triphosphate (ATP). Consistent with its ability to decrease ATP levels and induce cell death, lead also increased caspase-3 activity, an effect potentiated by glutamate. Exposure to glutamate and lead elevated the cellular malondialdehyde (MDA) levels and phospholipase-A 2 (PLA 2 ) activity and diminished the glutamine synthetase (GS) activity. NACA protected PC12 cells from the cytotoxic effects of glutamate plus lead, as evaluated by MTS assay. NACA reduced the decrease in the cellular ATP levels and restored the intracellular GSH levels. The increased levels of ROS and MDA in glutamate-lead treated cells were significantly decreased by NACA. In conclusion, our data showed that glutamate potentiated the effects of lead-induced PC12 cell death by a mechanism involving mitochondrial dysfunction (ATP depletion) and oxidative stress. NACA had a protective role against the combined toxic effects of glutamate and lead by inhibiting lipid peroxidation and scavenging ROS, thus preserving intracellular GSH

  13. N-Acetylcysteine in the Treatment of Pediatric Tourette Syndrome: Randomized, Double-Blind, Placebo-Controlled Add-On Trial. (United States)

    Bloch, Michael H; Panza, Kaitlyn E; Yaffa, Alisa; Alvarenga, Pedro G; Jakubovski, Ewgeni; Mulqueen, Jilian M; Landeros-Weisenberger, Angeli; Leckman, James F


    Current pharmacological treatments for Tourette Syndrome (TS), such as antipsychotic agents and α-2 agonists, are moderately effective in the treatment of tics, but have substantial side effects that limit their use. N-acetylcysteine (NAC) modulates glutamatergic systems, and has been used safely as an antioxidant agent with minimal side effects for decades. NAC has been increasingly studied for the treatment of other obsessive-compulsive spectrum disorders. We aim to examine the efficacy of NAC for the treatment of pediatric TS in a double-blind, placebo-controlled, add-on study. Thirty-one children and adolescents 8-17 years of age with TS were randomly assigned to receive NAC or matching placebo for 12 weeks. Our primary outcome was change in severity of tics as measured by the Yale Global Tic Severity Scale (YGTSS), Total tic score. Secondary measures assessed comorbid obsessive-compulsive disorder (OCD), depression, anxiety, and attention-deficit/hyperactivity disorder (ADHD). Linear mixed models in SAS were used to examine differences between NAC and placebo. Of 31 randomized subjects, 14 were assigned to placebo (two females; 11.5 + 2.8 years) and 17 to active NAC (five females; 12.4 + 1.4 years) treatment. No significant difference between NAC and placebo was found in reducing tic severity or any secondary outcomes. We found no evidence for efficacy of NAC in treating tic symptoms. Our findings stand in contrast to studies suggesting benefits of NAC in the treatment of other obsessive-compulsive spectrum disorders in adults, including OCD and trichotillomania, but are similar to a recent placebo-controlled trial of pediatric trichotillomania that found no benefit of NAC.

  14. Randomized, Double-Blind, Placebo-Controlled Trial of N-Acetylcysteine Augmentation for Treatment-Resistant Obsessive-Compulsive Disorder. (United States)

    Costa, Daniel L C; Diniz, Juliana B; Requena, Guaraci; Joaquim, Marinês A; Pittenger, Christopher; Bloch, Michael H; Miguel, Euripedes C; Shavitt, Roseli G


    To evaluate the efficacy of serotonin reuptake inhibitor (SRI) augmentation with N-acetylcysteine (NAC), a glutamate modulator and antioxidant medication, for treatment-resistant obsessive-compulsive disorder (OCD). We conducted a randomized, double-blind, placebo-controlled, 16-week trial of NAC (3,000 mg daily) in adults (aged 18-65 years) with treatment-resistant OCD, established according to DSM-IV criteria. Forty subjects were recruited at an OCD-specialized outpatient clinic at a tertiary hospital (May 2012-October 2014). The primary outcome measure was the Yale-Brown Obsessive Compulsive Scale (Y-BOCS) scores. To evaluate the variables group, time, and interaction effects for Y-BOCS scores at all time points, we used nonparametric analysis of variance with repeated measures. Secondary outcomes were the severity scores for anxiety, depression, specific OCD symptom dimensions, and insight. Both groups showed a significant reduction of baseline Y-BOCS scores at week 16: the NAC group had a reduction of 4.3 points (25.6 to 21.3), compared with 3.0 points (24.8 to 21.8) for the placebo group. However, there were no significant differences between groups (P = .92). Adding NAC was superior to placebo in reducing anxiety symptoms (P = .02), but not depression severity or specific OCD symptom dimensions. In general, NAC was well tolerated, despite abdominal pain being more frequently reported in the NAC group (n [%]: NAC = 9 [60.0], placebo = 2 [13.3]; P < .01). Our trial did not demonstrate a significant benefit of NAC in reducing OCD severity in treatment-resistant OCD adults. Secondary analysis suggested that NAC might have some benefit in reducing anxiety symptoms in treatment-resistant OCD patients. identifier: NCT01555970. © Copyright 2017 Physicians Postgraduate Press, Inc.

  15. N-acetylcysteine in agriculture, a novel use for an old molecule: focus on controlling the plant-pathogen Xylella fastidiosa.

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    Lígia S Muranaka

    Full Text Available Xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it causes Citrus Variegated Chlorosis (CVC. The mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. In this study, we investigated the inhibitory effect of N-Acetylcysteine (NAC, a cysteine analogue used mainly to treat human diseases, on X. fastidiosa in different experimental conditions. Concentrations of NAC over 1 mg/mL reduced bacterial adhesion to glass surfaces, biofilm formation and the amount of exopolysaccharides (EPS. The minimal inhibitory concentration of NAC was 6 mg/mL. NAC was supplied to X. fastidiosa-infected plants in hydroponics, fertigation, and adsorbed to organic fertilizer (NAC-Fertilizer. HPLC analysis indicated that plants absorbed NAC at concentrations of 0.48 and 2.4 mg/mL but not at 6 mg/mL. Sweet orange plants with CVC symptoms treated with NAC (0.48 and 2.4 mg/mL in hydroponics showed clear symptom remission and reduction in bacterial population, as analyzed by quantitative PCR and bacterial isolation. Experiments using fertigation and NAC-Fertilizer were done to simulate a condition closer to that normally is used in the field. For both, significant symptom remission and a reduced bacterial growth rate were observed. Using NAC-Fertilizer the lag for resurgence of symptoms on leaves after interruption of the treatment increased to around eight months. This is the first report of the anti-bacterial effect of NAC against a phytopathogenic bacterium. The results obtained in this work together with the characteristics of this molecule indicate that the use of NAC in agriculture might be a new and sustainable strategy for controlling plant pathogenic bacteria.

  16. Effect of fraxetin on antioxidant defense and stress proteins in human neuroblastoma cell model of rotenone neurotoxicity. Comparative study with myricetin and N-acetylcysteine

    International Nuclear Information System (INIS)

    Molina-Jimenez, Maria Francisca; Sanchez-Reus, Maria Isabel; Cascales, Maria; Andres, David; Benedi, Juana


    Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production. Recently, it has been shown that fraxetin (coumarin) and myricetin (flavonoid) have significant neuroprotective effects against apoptosis induced by rotenone, increase the total glutathione levels in vitro, and inhibit lipid peroxidation. Thus, these considerations prompted us to investigate the way in which fraxetin and myricetin affect the endogenous antioxidant defense system, such as Mn and CuZn superoxide dismutase (MnSOD, CuZnSOD), catalase, glutathione reductase (GR), and glutathione peroxidase (GPx) on rotenone neurotoxicity in neuroblastoma cells. N-acetylcysteine (NAC), a potent antioxidant, was employed as a comparative agent. Also, the expression and protein levels of HSP70 by Northern and Western blot analysis were assayed in SH-SY5Y cells. After incubation for 16 h, rotenone significantly increased the expression and activity of MnSOD, GPx, and catalase. When cells were preincubated with fraxetin, there was a decrease in the protein levels and activity of both MnSOD and catalase, in comparison with the rotenone treatment. The myricetin effect was less pronounced. Activity and expression of GPx were increased by rotenone and pre-treatment with fraxetin did not modify significantly these levels. The significant enhancement in HSP70 expression at mRNA and protein levels induced by fraxetin was observed by pre-treatment of cells 0.5 h before rotenone insult. These data suggest that major features of rotenone-induced neurotoxicity are partially mediated by free radical formation and oxidative stress, and that fraxetin partially protects against rotenone toxicity affecting the main protection system of the cells against oxidative injury

  17. Ex Vivo and in Vivo Evaluation of the Effect of Coating a Coumarin-6-Labeled Nanostructured Lipid Carrier with Chitosan-N-acetylcysteine on Rabbit Ocular Distribution. (United States)

    Liu, Dandan; Li, Jinyu; Cheng, Bingchao; Wu, Qingyin; Pan, Hao


    This study is focused on further understanding the characteristics of chitosan-N-acetylcysteine surface-modified nanostructured lipid carriers (CS-NAC-NLCs) in their interaction with ocular mucosa. Coumarin-6 (C6)-labeled NLCs, including uncoated NLCs, chitosan hydrochloride (CH)-, and CS-NAC-coated NLCs, were developed using a melt-emulsification technique and subsequently decorated with different types or portions of chitosan derivatives. Mucoadhesion was evaluated ex vivo using a flow-through process with fluorescence detection. The results demonstrated that the presence of CS-NAC on the C6-NLC surface provided the most obvious enhancement in adhesion due to the formation of both noncovalent (ionic) and covalent (disulfide bridges) interactions with mucus chains. Meanwhile, the concentration of CS-NAC in the formulation positively influenced the viscosity of the nanoparticles and hence prolonged their retention in the ocular tissue. Transcorneal penetration studies revealed that CS-NAC-NLC particles were able to penetrate through the entire corneal epithelium primarily via a transcellular route. The transport depth and velocity strongly relied on the modification material and the particle size. Ex vivo fluorescence imaging and in vivo ocular distribution investigations showed that C6 was broadly distributed in rabbit eye tissues and absorbed by aqueous humor after CS-NAC-NLC instillation. In relation to C6 eye drops, CS-NAC-NLCs achieved considerably higher C max (4.01-fold), MRT 0-∞ (1.87-fold), and AUC 0-∞ (16.29-fold) in the aqueous humor. Moreover, the increase in drug absorption was greater in the cornea than in the conjunctiva. Thereby, it is possible to draw a conclusion that CS-NAC-NLCs presented great potential for drug application to the front portion of the eye.

  18. N-Acetylcysteine-induced vasodilatation is modulated by KATP channels, Na+/K+-ATPase activity and intracellular calcium concentration: An in vitro study. (United States)

    Vezir, Özden; Çömelekoğlu, Ülkü; Sucu, Nehir; Yalın, Ali Erdinç; Yılmaz, Şakir Necat; Yalın, Serap; Söğüt, Fatma; Yaman, Selma; Kibar, Kezban; Akkapulu, Merih; Koç, Meryem İlkay; Seçer, Didem


    In this study, we aimed to investigate the role of ATP-sensitive potassium (K ATP ) channel, Na + /K + -ATPase activity, and intracellular calcium levels on the vasodilatory effect of N-acetylcysteine (NAC) in thoracic aorta by using electrophysiological and molecular techniques. Rat thoracic aorta ring preparations and cultured thoracic aorta cells were divided into four groups as control, 2mM NAC, 5mM NAC, and 10mM NAC. Thoracic aorta rings were isolated from rats for measurements of relaxation responses and Na + /K + -ATPase activity. In the cultured thoracic aorta cells, we measured the currents of K ATP channel, the concentration of intracellular calcium and mRNA expression level of K ATP channel subunits (KCNJ8, KCNJ11, ABCC8 and ABCC9). The relaxation rate significantly increased in all NAC groups compared to control. Similarly, Na + /K + - ATPase activity also significantly decreased in NAC groups. Outward K ATP channel current significantly increased in all NAC groups compared to the control group. Intracellular calcium concentration decreased significantly in all groups with compared control. mRNA expression level of ABCC8 subunit significantly increased in all NAC groups compared to the control group. Pearson correlation analysis showed that relaxation rate was significantly associated with K ATP current, intracellular calcium concentration, Na + /K + -ATPase activity and mRNA expression level of ABCC8 subunit. Our findings suggest that NAC relaxes vascular smooth muscle cells through a direct effect on K ATP channels, by increasing outward K+ flux, partly by increasing mRNA expression of K ATP subunit ABCC8, by decreasing in intracellular calcium and by decreasing in Na + /K + -ATPase activity. Copyright © 2017 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  19. N-acetylcysteine attenuates hexavalent chromium-induced hypersensitivity through inhibition of cell death, ROS-related signaling and cytokine expression.

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    Yu-Hsuan Lee

    Full Text Available Chromium hypersensitivity (chromium-induced allergic contact dermatitis is an important issue in occupational skin disease. Hexavalent chromium (Cr (VI can activate the Akt, Nuclear factor κB (NF-κB, and Mitogen-activated protein kinase (MAPK pathways and induce cell death, via the effects of reactive oxygen species (ROS. Recently, cell death stimuli have been proposed to regulate the release of inflammatory cytokines, such as tumor necrosis factor-α (TNF-α and interleukin-1 (IL-1. However, the exact effects of ROS on the signaling molecules and cytotoxicity involved in Cr(VI-induced hypersensitivity have not yet been fully demonstrated. N-acetylcysteine (NAC could increase glutathione levels in the skin and act as an antioxidant. In this study, we investigated the effects of NAC on attenuating the Cr(VI-triggered ROS signaling in both normal keratinocyte cells (HaCaT cells and a guinea pig (GP model. The results showed the induction of apoptosis, autophagy and ROS were observed after different concentrations of Cr(VI treatment. HaCaT cells pretreated with NAC exhibited a decrease in apoptosis and autophagy, which could affect cell viability. In addition, Cr (VI activated the Akt, NF-κB and MAPK pathways thereby increasing IL-1α and TNF-α production. However, all of these stimulation phenomena could be inhibited by NAC in both of in vitro and in vivo studies. These novel findings indicate that NAC may prevent the development of chromium hypersensitivity by inhibiting of ROS-induced cell death and cytokine expression.

  20. Calcium-dependent nitric oxide production is involved in the cytoprotective properties of n-acetylcysteine in glycochenodeoxycholic acid-induced cell death in hepatocytes

    International Nuclear Information System (INIS)

    Gonzalez-Rubio, Sandra; Linares, Clara I.; Bello, Rosario I.; Gonzalez, Raul; Ferrin, Gustavo; Hidalgo, Ana B.; Munoz-Gomariz, Elisa; Rodriguez, Blanca A.; Barrera, Pilar; Ranchal, Isidora; Duran-Prado, Mario; Aguilar-Melero, Patricia; De la Mata, Manuel; Muntane, Jordi


    The intracellular oxidative stress has been involved in bile acid-induced cell death in hepatocytes. Nitric oxide (NO) exerts cytoprotective properties in glycochenodeoxycholic acid (GCDCA)-treated hepatocytes. The study evaluated the involvement of Ca 2+ on the regulation of NO synthase (NOS)-3 expression during N-acetylcysteine (NAC) cytoprotection against GCDCA-induced cell death in hepatocytes. The regulation of Ca 2+ pools (EGTA or BAPTA-AM) and NO (L-NAME or NO donor) production was assessed during NAC cytoprotection in GCDCA-treated HepG2 cells. The stimulation of Ca 2+ entrance was induced by A23187 in HepG2. Cell death, Ca 2+ mobilization, NOS-1, -2 and -3 expression, AP-1 activation, and NO production were evaluated. GCDCA reduced intracellular Ca 2+ concentration and NOS-3 expression, and enhanced cell death in HepG2. NO donor prevented, and L-NAME enhanced, GCDCA-induced cell death. The reduction of Ca 2+ entry by EGTA, but not its release from intracellular stores by BAPTA-AM, enhanced cell death in GCDCA-treated cells. The stimulation of Ca 2+ entrance by A23187 reduced cell death and enhanced NOS-3 expression in GCDCA-treated HepG2 cells. The cytoprotective properties of NAC were related to the recovery of intracellular Ca 2+ concentration, NOS-3 expression and NO production induced by GCDCA-treated HepG2 cells. The increase of NO production by Ca 2+ -dependent NOS-3 expression during NAC administration reduces cell death in GCDCA-treated hepatocytes.

  1. Calcium-dependent nitric oxide production is involved in the cytoprotective properties of n-acetylcysteine in glycochenodeoxycholic acid-induced cell death in hepatocytes

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    Gonzalez-Rubio, Sandra; Linares, Clara I; Bello, Rosario I [Liver Research Unit, Reina Sofia University Hospital, Cordoba (Spain); Gonzalez, Raul; Ferrin, Gustavo [Liver Research Unit, Reina Sofia University Hospital, Cordoba (Spain); Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas (CIBEREH o Ciberehd) (Spain); Hidalgo, Ana B [Liver Research Unit, Reina Sofia University Hospital, Cordoba (Spain); Munoz-Gomariz, Elisa [Department of Biostatistics, Reina Sofia University Hospital, Cordoba (Spain); Rodriguez, Blanca A [Liver Research Unit, Reina Sofia University Hospital, Cordoba (Spain); Barrera, Pilar; Ranchal, Isidora [Liver Research Unit, Reina Sofia University Hospital, Cordoba (Spain); Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas (CIBEREH o Ciberehd) (Spain); Duran-Prado, Mario [Instituto de Parasitologia y Biomedicina Lopez Neyra, CSIC, Granada (Spain); CIBER Fisiopatologia de la Obesidad y Nutricion CB06/03, Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo (Spain); Aguilar-Melero, Patricia [Liver Research Unit, Reina Sofia University Hospital, Cordoba (Spain); De la Mata, Manuel [Liver Research Unit, Reina Sofia University Hospital, Cordoba (Spain); Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas (CIBEREH o Ciberehd) (Spain); Muntane, Jordi [Liver Research Unit, Reina Sofia University Hospital, Cordoba (Spain); Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas (CIBEREH o Ciberehd) (Spain)


    The intracellular oxidative stress has been involved in bile acid-induced cell death in hepatocytes. Nitric oxide (NO) exerts cytoprotective properties in glycochenodeoxycholic acid (GCDCA)-treated hepatocytes. The study evaluated the involvement of Ca{sup 2+} on the regulation of NO synthase (NOS)-3 expression during N-acetylcysteine (NAC) cytoprotection against GCDCA-induced cell death in hepatocytes. The regulation of Ca{sup 2+} pools (EGTA or BAPTA-AM) and NO (L-NAME or NO donor) production was assessed during NAC cytoprotection in GCDCA-treated HepG2 cells. The stimulation of Ca{sup 2+} entrance was induced by A23187 in HepG2. Cell death, Ca{sup 2+} mobilization, NOS-1, -2 and -3 expression, AP-1 activation, and NO production were evaluated. GCDCA reduced intracellular Ca{sup 2+} concentration and NOS-3 expression, and enhanced cell death in HepG2. NO donor prevented, and L-NAME enhanced, GCDCA-induced cell death. The reduction of Ca{sup 2+} entry by EGTA, but not its release from intracellular stores by BAPTA-AM, enhanced cell death in GCDCA-treated cells. The stimulation of Ca{sup 2+} entrance by A23187 reduced cell death and enhanced NOS-3 expression in GCDCA-treated HepG2 cells. The cytoprotective properties of NAC were related to the recovery of intracellular Ca{sup 2+} concentration, NOS-3 expression and NO production induced by GCDCA-treated HepG2 cells. The increase of NO production by Ca{sup 2+}-dependent NOS-3 expression during NAC administration reduces cell death in GCDCA-treated hepatocytes.

  2. N-acetylcysteine in a Double-Blind Randomized Placebo-Controlled Trial: Toward Biomarker-Guided Treatment in Early Psychosis (United States)

    Conus, Philippe; Seidman, Larry J; Fournier, Margot; Xin, Lijing; Cleusix, Martine; Baumann, Philipp S; Ferrari, Carina; Cousins, Ann; Alameda, Luis; Gholam-Rezaee, Mehdi; Golay, Philippe; Jenni, Raoul; Woo, T -U Wilson; Keshavan, Matcheri S; Eap, Chin B; Wojcik, Joanne; Cuenod, Michel; Buclin, Thierry; Gruetter, Rolf


    Abstract Biomarker-guided treatments are needed in psychiatry, and previous data suggest oxidative stress may be a target in schizophrenia. A previous add-on trial with the antioxidant N-acetylcysteine (NAC) led to negative symptom reductions in chronic patients. We aim to study NAC’s impact on symptoms and neurocognition in early psychosis (EP) and to explore whether glutathione (GSH)/redox markers could represent valid biomarkers to guide treatment. In a double-blind, randomized, placebo-controlled trial in 63 EP patients, we assessed the effect of NAC supplementation (2700 mg/day, 6 months) on PANSS, neurocognition, and redox markers (brain GSH [GSHmPFC], blood cells GSH levels [GSHBC], GSH peroxidase activity [GPxBC]). No changes in negative or positive symptoms or functional outcome were observed with NAC, but significant improvements were found in favor of NAC on neurocognition (processing speed). NAC also led to increases of GSHmPFC by 23% (P = .005) and GSHBC by 19% (P = .05). In patients with high-baseline GPxBC compared to low-baseline GPxBC, subgroup explorations revealed a link between changes of positive symptoms and changes of redox status with NAC. In conclusion, NAC supplementation in a limited sample of EP patients did not improve negative symptoms, which were at modest baseline levels. However, NAC led to some neurocognitive improvements and an increase in brain GSH levels, indicating good target engagement. Blood GPx activity, a redox peripheral index associated with brain GSH levels, could help identify a subgroup of patients who improve their positive symptoms with NAC. Thus, future trials with antioxidants in EP should consider biomarker-guided treatment. PMID:29462456

  3. Impact of N-acetylcysteine on antitumor activity of doxorubicin and landomycin A in NK/Ly lymphoma-bearing mice

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    Yu. S. Kozak


    Full Text Available N-acetylcysteine (NAC is a dietary supplement demonstrating antioxidant and liver protecting effects that is widely used in clinics. NAC is considered to possess potential therapeutic activity for health disorders characterized by generation of free oxygen radicals, as well as potential for decreasing negative side effects of various drugs. However, the mechanisms of such tissue-protective actions of NAC remain poorly understood. The main aim of this work was to study therapeutic effects of NAC applied together with the “gold standard” of chemotherapy doxorubicin (Dx or the novel experimental drug landomycin A (LA to mice bearing NK/Ly lymphoma. It was revealed that NAC significantly decreased the nephrotoxicity of Dx (measured as creatinine level, possessed moderate immunomodulating activity (as revealed by an increase in number of cytotoxic T-lymphocytes, and partially increased survival of NK/Ly lymphoma-bearing animals treated with Dx. On the contrary, there was little tissue-protective effect of NAC towards LA due to the weak side effects of this anticancer drug, however, the combined use of NAC and LA significantly increased survival (60+ days of LA-treated animals with NK/Ly lymphoma. Summarizing, NAC possesses a moderate tissue-protective activity towards Dx action but lacks a major therapeutic effect. However, in the case of LA action, NAC significantly increases its anticancer activity with no impact on its negative side effects. Further studies of the molecular mechanisms underlying that activity of NAC towards the action of LA are in progress.

  4. N-acetylcysteine increases the frequency of bone marrow pro-B/pre-B cells, but does not reverse cigarette smoking-induced loss of this subset.

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    Victoria L Palmer

    Full Text Available We previously showed that mice exposed to cigarette smoke for three weeks exhibit loss of bone marrow B cells at the Pro-B-to-pre-B cell transition, but the reason for this is unclear. The antioxidant N-acetylcysteine (NAC, a glutathione precursor, has been used as a chemopreventive agent to reduce adverse effects of cigarette smoke exposure on lung function. Here we determined whether smoke exposure impairs B cell development by inducing cell cycle arrest or apoptosis, and whether NAC treatment prevents smoking-induced loss of developing B cells.Groups of normal mice were either exposed to filtered room air or cigarette smoke with or without concomitant NAC treatment for 5 days/week for three weeks. Bone marrow B cell developmental subsets were enumerated, and sorted pro-B (B220(+CD43(+ and pre-B (B220(+CD43(- cell fractions were analyzed for cell cycle status and the percentage of apoptotic cells. We find that, compared to sham controls, smoke-exposed mice have ∼60% fewer pro-B/pre-B cells, regardless of NAC treatment. Interestingly, NAC-treated mice show a 21-38% increase in total bone marrow cellularity and lymphocyte frequency and about a 2-fold increase in the pro-B/pre-B cell subset, compared to sham-treated controls. No significant smoking- or NAC-dependent differences were detected in frequency of apoptotic cells or the percentage cells in the G1, S, or G2 phases of the cycle.The failure of NAC treatment to prevent smoking-induced loss of bone marrow pre-B cells suggests that oxidative stress is not directly responsible for this loss. The unexpected expansion of the pro-B/pre-B cell subset in response to NAC treatment suggests oxidative stress normally contributes to cell loss at this developmental stage, and also reveals a potential side effect of therapeutic administration of NAC to prevent smoking-induced loss of lung function.

  5. Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine. (United States)

    Orue, Andrea; Chavez, Valery; Strasberg-Rieber, Mary; Rieber, Manuel


    The metabolic inhibitor 3-bromopyruvate (3-BrPA) is a promising anti-cancer alkylating agent, shown to inhibit growth of some colorectal carcinoma with KRAS mutation. Recently, we demonstrated increased resistance to 3-BrPA in wt p53 tumor cells compared to those with p53 silencing or mutation. Since hypoxic microenvironments select for tumor cells with diminished therapeutic response, we investigated whether hypoxia unequally increases resistance to 3-BrPA in wt p53 MelJuso melanoma harbouring (Q61L)-mutant NRAS and wt BRAF, C8161 melanoma with (G12D)-mutant KRAS (G464E)-mutant BRAF, and A549 lung carcinoma with a KRAS (G12S)-mutation. Since hypoxia increases the toxicity of the p53 activator, Prima-1 against breast cancer cells irrespective of their p53 status, we also investigated whether Prima-1 reversed hypoxic resistance to 3-BrPA. In contrast to the high susceptibility of hypoxic mutant NRAS MelJuso cells to 3-BrPA or Prima-1, KRAS mutant C8161 and A549 cells revealed hypoxic resistance to 3-BrPA counteracted by Prima-1. In A549 cells, Prima-1 increased p21CDKN1mRNA, and reciprocally inhibited mRNA expression of the SLC2A1-GLUT1 glucose transporter-1 and ALDH1A1, gene linked to detoxification and stem cell properties. 3-BrPA lowered CAIX and VEGF mRNA expression. Death from joint Prima-1 and 3-BrPA treatment in KRAS mutant A549 and C8161 cells seemed mediated by potentiating oxidative stress, since it was antagonized by the anti-oxidant and glutathione precursor N-acetylcysteine. This report is the first to show that Prima-1 kills hypoxic wt p53 KRAS-mutant cells resistant to 3-BrPA, partly by decreasing GLUT-1 expression and exacerbating pro-oxidant stress.

  6. Pre-treatment with N-acetylcysteine upregulates superoxide dismutase 2 and catalase genes in cadmium-induced oxidative stress in the chick omphalocele model. (United States)

    Doi, Takashi; Puri, Prem; Bannigan, John; Thompson, Jennifer


    In the chick embryo, administration of the heavy metal Cadmium (Cd) induces omphalocele phenotype. Cd is a potent inhibitor of antioxidant enzymes and causes accumulation of reactive oxygen species (ROSs) such as hydrogen peroxide. Previous work with the Cd chick model has demonstrated that increased levels of MDA, as a marker for oxidative stress, 24 h post Cd treatment (24H) are identical in chick embryos exposed to Cd. Furthermore, of the several antioxidants assessed, only N-acetylcysteine (NAC) has been shown to reduce MDA levels to control values in the Cd-treated chick embryo. However, the molecular mechanisms by which NAC acts to maintain oxidative stress in the Cd-induced ventral body wall defect chick model remains to be unclear. We designed this study to investigate the hypothesis that gene expression levels of antioxidant enzymes are downregulated in malformed embryos exposed to Cd compared to controls and to determine the effect of pre-treatment with NAC on the expression levels of genes encoding antioxidant enzymes. After 60 h incubation, chick embryos were pre-treated with NAC and exposed to either chick saline or Cd. Chicks were then harvested at 24H and divided into five groups: control, Cd group without malformation [Cd(-)], Cd group with malformation [Cd(+)], NAC + Cd(-) and NAC + Cd(+). Real-time PCR was performed to evaluate the relative mRNA expression levels of antioxidant enzymes, including superoxide dismutase (SOD)-1, SOD2, catalase (CAT) and glutathione peroxidase (GPX)-4. Differences between five groups were tested by Tukey-Kramer post-hoc test following one-way ANOVA. Statistical significance was accepted at p < 0.05. Immunohistochemistry was also performed to evaluate protein expression. The mRNA expression levels of SOD2 and CAT were significantly decreased in Cd(+) as compared to controls, whereas there was no significant difference between controls and Cd(-) (p < 0.05 vs. controls). In addition, gene expression levels of

  7. 1,4-Anthraquinone: A new useful pre-column reagent for the determination of N-acetylcysteine and captopril in pharmaceuticals by high performance liquid chromatography. (United States)

    Gatti, Rita; Morigi, Rita


    1,4-Anthraquinone (ANQ) is proposed as a novel pre-column reagent for high performance liquid chromatography (HPLC) determination of N-acetylcysteine (NAC) and captopril (CAP) in pharmaceutical formulations. The derivatization reactions were carried out at room temperature: NAC at pH 8 for 1min, while CAP at pH 7.5 for 20min. Both reactions reached completeness at a reagent to thiol molar ratio of about 2.5. The synthesised derivatives were characterized by 1 H NMR and IR. The chromatographic separations were performed on a C 18 Phenomenex Synergi Fusion 4μm (250mm×4.6mm I.D.) stainless steel column with detection at λ=300nm. The mobile phase consisted of methanol/triethylammonium (TEA) phosphate buffer (pH 3; 0.05mol/L) 75:25 (v/v) at a flow-rate of 0.4mL/min for NAC and 88:12 (v/v), at a flow-rate of 0.6mL/min for CAP. The validation parameters (linearity, sensitivity, accuracy, precision, specificity and stability) were highly satisfactory. Linear response was observed (determination coefficient ≥0.9996). Detection limits were about 8 and 18ng/mL for NAC and CAP, respectively. Intra-day precision (relative standard deviation, R.S.D.) was ≤1.58%, for thiol to internal standard (IS) peak area ratio and ≤0.33%, for thiol and IS retention times (t R ), without significant differences between intra- and inter-day data. Thiol recovery studies were satisfactory (99.50%) with R.S.D. ≤0.56%. The results highlight the high sensitivity of the method and the remarkable reactivity and selectivity of the reagent towards the thiol function. The developed method is suitable for the quality control of both thiols in commercial products. The method can be applied in any analytical laboratory not requiring a sophisticated instrumentation. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Phase I randomized clinical trial of N-acetylcysteine in combination with an adjuvant probenecid for treatment of severe traumatic brain injury in children.

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    Robert S B Clark

    Full Text Available There are no therapies shown to improve outcome after severe traumatic brain injury (TBI in humans, a leading cause of morbidity and mortality. We sought to verify brain exposure of the systemically administered antioxidant N-acetylcysteine (NAC and the synergistic adjuvant probenecid, and identify adverse effects of this drug combination after severe TBI in children.IRB-approved, randomized, double-blind, placebo controlled Phase I study in children 2 to 18 years-of-age admitted to a Pediatric Intensive Care Unit after severe TBI (Glasgow Coma Scale [GCS] score ≤8 requiring an externalized ventricular drain for measurement of intracranial pressure (ICP. Patients were recruited from November 2011-August 2013. Fourteen patients (n = 7/group were randomly assigned after obtaining informed consent to receive probenecid (25 mg/kg load, then 10 mg/kg/dose q6h×11 doses and NAC (140 mg/kg load, then 70 mg/kg/dose q4h×17 doses, or placebos via naso/orogastric tube. Serum and CSF samples were drawn pre-bolus and 1-96 h after randomization and drug concentrations were measured via UPLC-MS/MS. Glasgow Outcome Scale (GOS score was assessed at 3 months.There were no adverse events attributable to drug treatment. One patient in the placebo group was withdrawn due to adverse effects. In the treatment group, NAC concentrations ranged from 16,977.3±2,212.3 to 16,786.1±3,285.3 in serum and from 269.3±113.0 to 467.9±262.7 ng/mL in CSF, at 24 to 72 h post-bolus, respectively; and probenecid concentrations ranged from 75.4.3±10.0 to 52.9±25.8 in serum and 5.4±1.0 to 4.6±2.1 μg/mL in CSF, at 24 to 72 h post-bolus, respectively (mean±SEM. Temperature, mean arterial pressure, ICP, use of ICP-directed therapies, surveillance serum brain injury biomarkers, and GOS at 3 months were not different between groups.Treatment resulted in detectable concentrations of NAC and probenecid in CSF and was not associated with undesirable effects after TBI in children

  9. Metabolomic Analysis of N-acetylcysteine Protection of Injury from Gadolinium-DTPA Contrast Agent in Rats with Chronic Renal Failure. (United States)

    Wan, Chuanling; Xue, Rong; Zhan, Youyang; Wu, Yijie; Li, Xiaojing; Pei, Fengkui


    Gadolinium-based contrast agents (GBCAs) are frequently used to enhance the diagnostic efficacy of magnetic resonance imaging. On the other hand, the association between GBCA administration in patients with advanced renal disease and nephrogenic systemic fibrosis (NSF) was also noted. NSF is a systemic disorder characterized by widespread tissue fibrosis that may lead to death. N-acetylcysteine (NAC) protects rats from injury induced by gadolinium-based contrast agents, but the underlying mechanisms remain unclear. In this study, a nuclear magnetic resonance-based metabolomic approach was used to systematically investigate the protective effects of NAC on Gd-DTPA-induced injury. Thirty-two male Sprague-Dawley rats were given adenine (200 mg·kg -1 body weight) by oral gavage once a day for 3 weeks to induce chronic renal failure (CRF). NAC (600 mg/L in drinking water for 9 days) pretreatment was initiated 2 days before Gd-DTPA injection (a single tail vein injection, 2 mmol/kg body weight). Serum and liver samples were collected on day 7 after Gd-DTPA injection. By study design, the serum and hepatic metabolic changes of rats were measured in four groups of eight each: CRF, CRF-Gd, CRF-Gd-NAC, and CRF-NAC. Gd-DTPA administration to rats with CRF resulted in disturbances of several metabolic pathways, including glucose, lipid, glutamate, choline, gut microbiota, one-carbon, and purine metabolism. NAC pretreatment reversed the abundance changes of high-density lipoprotein, low-density lipoprotein, very low-density lipoprotein, glutamate, glutamine, oxidized glutathione, choline, phosphocholine, glycerophosphocholine, trimethylamine, and trimethylamine-N-oxide induced by Gd-DTPA. It is noteworthy, however, that the ameliorating effects of NAC on the disturbance of glutamate, choline, and gut microbiota metabolism may be specific to Gd-DTPA. In all, these findings could be potentially useful to decipher the underlying mechanisms of NAC protective effects from the

  10. Do intravenous N-acetylcysteine and sodium bicarbonate prevent high osmolal contrast-induced acute kidney injury? A randomized controlled trial.

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    Antonio Jose Inda-Filho

    Full Text Available N-acetylcysteine (NAC or sodium bicarbonate (NaHCO3, singly or combined, inconsistently prevent patients exposed to radiographic contrast media from developing contrast-induced acute kidney injury (CI-AKI.We asked whether intravenous isotonic saline and either NaHCO3 in 5% dextrose or else a high dose of NAC in 5% dextrose prevent CI-AKI in outpatients exposed to high-osmolal iodinated contrast medium more than does saline alone.This completed prospective, parallel, superiority, open-label, controlled, computer-randomized, single-center, Brazilian trial (NCT01612013 hydrated 500 adult outpatients (214 at high risk of developing CI-AKI exposed to ioxitalamate during elective coronary angiography and ventriculography. From 1 hour before through 6 hours after exposure, 126 patients (group 1 received a high dose of NAC and saline, 125 (group 2 received NaHCO3 and saline, 124 (group 3 received both treatments, and 125 (group 4 received only saline.Groups were similar with respect to age, gender, weight, pre-existing renal dysfunction, hypertension, medication, and baseline serum creatinine and serum cystatin C, but diabetes mellitus was significantly less prevalent in group 1. CI-AKI incidence 72 hours after exposure to contrast medium was 51.4% (257/500, measured as serum creatinine > (baseline+0.3 mg/dL and/or serum cystatin C > (1.1 · baseline, and 7.6% (38/500, measured as both serum creatinine and serum cystatin C > (baseline+0.3 mg/dL or > (1.25 · baseline. CI-AKI incidence measured less sensitively was similar among groups. Measured more sensitively, incidence in group 1 was significantly (p<0.05 lower than in groups 2 and 3 but not group 4; adjustment for confounding by infused volume equalized incidence in groups 1 and 3.We found no evidence that intravenous isotonic saline and either NaHCO3 or else a high dose of NAC prevent CI-AKI in outpatients exposed to high osmolal iodinated contrast medium more than does saline alone

  11. Chelating capacity and the adverse effects of two treatments (N-acetylcysteine and D-penicillamine in patients with mercury poisoning in Segovia, a municipality at the northeastern part of Antioquia, Colombia

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    Fanny Cuesta González


    Full Text Available

    OBJECTIVE: to compare the chelating capacity and the adverse effects of treatments with either Nacetylcysteine or D-penicillamine in patients with mercury poisoning in Segovia, a municipality at the northeastern part of Antioquia, Colombia.

    METHODS: 50 patients with toxic levels of mercury were enrolled in a 10 days open label, randomized comparison of either D-penicillamine (750 mg/day or Nacetilcysteine (1.8 g/day. Patients were followed on a daily basis to assess the elimination of mercury in urine and the frequency of adverse effects of each treatment.

    RESULTS: 32 patients completed 10 days of drug treatment. Averages of mercury elimination in 24 hours urine, before and after treatment with D-penicillamine and N-acetylcysteine, were not different (211.96 mcg ± 190 and 262.15 mcg ± 305 and 232.85 mcg ± 248 and 218.65 mcg ± 240, respectively, P > 0.05 for all comparisons. Evaluation of the frequency of adverse effects showed a significant difference between the two groups: D-penicillamine (50% and N-acetylcysteine (11% p = 0.0079.

    CONCLUSION: this study

  12. Labelling, biodistribution and compartmental analysis of N-acetylcysteine labelled with Tc-99m. Comparative investigation with with 99m Tc-MIBI in an in vivo tumoral model

    International Nuclear Information System (INIS)

    Faintuch, Bluma Linkowski


    Labelling and biodistribution studies were done with two different ligands, respectively Methoxy isobutyl isonitrile (MIBI) and N-acetylcysteine (NAC), employing Tc-99m as a tracer. The main objective was to assess the pharmacokinetic properties of the second substance, aiming at its possible application in cancer diagnosis. To this purpose an in vivo investigation was done using healthy and tumor-bearing rats with experimental cancer. Images of tumor-bearing rats registered in a scintillation camera indicated that with 99m Tc-MIBI none of the two selected times was adequate for visualization of the cancer mass. In contrast, 99m Tc-NAC permitted clear identification of the humor, four hours after injection. The results have demonstrated that 99m Tc-NAC is a radiopharmaceutical with affinity for cancer tissue and promising for further investigation concerning imaging diagnosis of tumors. (author)

  13. Maternal melatonin or N-acetylcysteine therapy regulates hydrogen sulfide-generating pathway and renal transcriptome to prevent prenatal NG-Nitro-L-arginine-methyl ester (L-NAME)-induced fetal programming of hypertension in adult male offspring. (United States)

    Tain, You-Lin; Lee, Chien-Te; Chan, Julie Y H; Hsu, Chien-Ning


    Pregnancy is a critical time for fetal programming of hypertension. Nitric oxide deficiency during pregnancy causes hypertension in adult offspring. We examined whether maternal melatonin or N-acetylcysteine therapy can prevent N G -nitro-L-arginine-methyl ester-induced fetal programming of hypertension in adult offspring. Next, we aimed to identify potential gatekeeper pathways that contribute to N G -nitro-L-arginine-methyl ester -induced programmed hypertension using the next generation RNA sequencing technology. Pregnant Sprague-Dawley rats were assigned to 4 groups: control, N G -nitro-L-arginine-methyl ester, N G -nitro-L-arginine-methyl ester +melatonin, and N G -nitro-L-arginine-methyl ester+N-acetylcysteine. Pregnant rats received N G -nitro-L-arginine-methyl ester administration at 60 mg/kg/d subcutaneously during pregnancy alone, with additional 0.01% melatonin in drinking water, or with additional 1% N-acetylcysteine in drinking water during the entire pregnancy and lactation. Male offspring (n=8/group) were killed at 12 weeks of age. N G -nitro-L-arginine-methyl ester exposure during pregnancy induced programmed hypertension in adult male offspring, which was prevented by maternal melatonin or N-acetylcysteine therapy. Protective effects of melatonin and N-acetylcysteine against N G -nitro-L-arginine-methyl ester-induced programmed hypertension were associated with an increase in hydrogen sulfide-generating enzymes and hydrogen sulfide synthesis in the kidneys. Nitric oxide inhibition by N G -nitro-L-arginine-methyl ester in pregnancy caused >2000 renal transcripts to be modified during nephrogenesis stage in 1-day-old offspring kidney. Among them, genes belong to the renin-angiotensin system, and arachidonic acid metabolism pathways were potentially involved in the N G -nitro-L-arginine-methyl ester-induced programmed hypertension. However, melatonin and N-acetylcysteine reprogrammed the renin-angiotensin system and arachidonic acid pathway

  14. The effect of short-term, high-dose oral N-acetylcysteine treatment on oxidative stress markers in cystic fibrosis patients with chronic P. aeruginosa infection -- a pilot study. (United States)

    Skov, Marianne; Pressler, Tacjana; Lykkesfeldt, Jens; Poulsen, Henrik Enghusen; Jensen, Peter Østrup; Johansen, Helle Krogh; Qvist, Tavs; Kræmer, Dorthe; Høiby, Niels; Ciofu, Oana


    Patients with cystic fibrosis (CF) and chronic Pseudomonas aeruginosa lung infection have increased oxidative stress as a result of an imbalance between the production of reactive oxygen species caused by inflammation and their inactivation by the impaired antioxidant systems. Supplementation with anti-oxidants is potentially beneficial for CF patients. The effect of 4 weeks of oral N-acetylcysteine (NAC) treatment (2400 mg/day divided into two doses) on biochemical parameters of oxidative stress was investigated in an open-label, controlled, randomized trial on 21 patients; 11 patients in the NAC group and 10 in the control group. Biochemical parameters of oxidative burden and plasma levels of antioxidants were assessed at the end of the study and compared to the baseline values in the two groups. A significant increase in the plasma levels of the antioxidant ascorbic acid (p=0.037) and a significant decrease in the levels of the oxidized form of ascorbic acid (dehydroascorbate) (p=0.004) compared to baseline were achieved after NAC treatment. No significant differences were observed in the control group. The parameters of oxidative burden did not change significantly compared to baseline in either of the groups. A better lung function was observed in the NAC treated group with a mean (SD) change compared to baseline of FEV1% predicted of 2.11 (4.6), while a decrease was observed in the control group (change -1.4 (4.6)), though not statistically significant. Treatment with N-acetylcysteine 1200 mg×2/day for 30 days significantly decreased the level of oxidized vitamin C and increased the level of vitamin C (primary end-points) and a not statistically significant improvement of lung function was observed in this group of patients. Copyright © 2014 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

  15. Prospective study to compare antibiosis versus the association of N-acetylcysteine, D-mannose and Morinda citrifolia fruit extract in preventing urinary tract infections in patients submitted to urodynamic investigation

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    Giovanni Palleschi


    Full Text Available Background: The abuse of antimicrobical drugs has increased the resistance of microorganisms to treatments, thus to make urinary tract infections (UTIs more difficult to eradicate. Among natural substances used to prevent UTI, literature has provided preliminary data of the beneficial effects of D-mannose, N-acetylcysteine, and Morinda citrifolia fruit extract, due to their complementary mechanism of action which contributes respectively to limit bacteria adhesion to the urothelium, to destroy bacterial pathogenic biofilm, and to the anti-inflammatory and analgesic activity. The purpose of this study was to compare the administration of an association of D-mannose, N-acetylcysteine (NAC and Morinda citrifolia extract versus antibiotic therapy in the prophylaxis of UTIs potentially associated with urological mini-invasive diagnostics procedures, in clinical model of the urodynamic investigation. Methods: 80 patients eligible for urodynamic examination, 42 men and 38 women, have been prospectively enrolled in the study and randomised in two groups (A and B of 40 individuals. Patients of group A followed antibiotic therapy with Prulifloxacine, by mouth 400 mg/day for 5 days, while patients of the group B followed the association of mannose and NAC therapy, two vials/day for 7 days. Ten days after the urodynamic study, the patients were submitted to urine examination and urine culture. Results: The follow up assessment didn't show statistical significant difference between the two groups regarding the incidence of UTI. Conclusions: The association of mannose and NAC therapy resulted similar to the antibiotic therapy in preventing UTIs in patients submitted to urodynamic examination. This result leads to consider the possible use of these nutraceutical agents as a good alternative in the prophylaxis of the UTI afterwards urological procedures in urodynamics.

  16. Ausländerfeindlichkeit unter Jugendlichen in Leipzig


    Petermann, Sören; Goldenberg, Olga


    In kommunalen Umfragen erhobene Einstellungen gegenüber Ausländern repräsentieren ein Bild gelebter Toleranz. Selten werden Jugendliche zu ihren ausländerbezogenen Einstellungen gefragt und mit altersspezifischen Faktoren in Verbindung gesetzt. Dieser Beitrag bezieht sich auf theoretische Ansätze der Anomietheorie, der Sozialisations- und Deprivationsforschung, um Kompensationsprozesse und Konfliktpotenziale als Wirkungsmechanismen für Ausländerfeindlichkeit unter Leipziger Jugendlichen zu un...

  17. N-乙酰半胱氨酸治疗社区获得性肺炎的效果%Effect Observation of N-acetylcysteine in Treatment of Community Acquired Pneumonia

    Institute of Scientific and Technical Information of China (English)

    董晓娜; 李欣欣; 张静; 张振安; 张风林


    Objective To investigate the clinical effect of N-acetylcysteine in treatment of community acquired pneumonia.Methods Total of 156 patients with community acquired pneumonia were selected in Tangshan People′s Hospital from Nov.2012 to Nov.2014,and then divided into a conventional group (78 cases) and N-acetylcysteine group(78 cases) by random number method.The conventional group received conventional treatment.N-acetylcysteine group received N-acetylcysteine (100 mg every time,thrice a day) on the basis of conventional treatment.The pyretolysis time,antibiotics use time,hospitalization time,inflam-matory factors before and after treatment,levels of immune indexes,changes of serum calcitonin,partial pres-sure of oxygen(PaO2),partial pressure of carbon dioxide(PaCO2) of the two groups were compared.Results Pyretolysis time,antibiotics use time,hospitalization time of the N-acetylcysteine group was significantly less than the conventional group[(3.8 ±0.7) d vs (4.6 ±1.0) d,(12.5 ±2.4) d vs (16.1 ±3.6) d, (17.2 ±3.5) d vs (21.4 ±2.8) d,P <0.01],while the levels of high-sensitivity C-reactive protein (hs-CRP),interleukin-6 (IL-6),tumor necrosis factor-α(TNF-α),white blood cell count(WBC),neutro-phil,procalcitonin,PaCO2 were significantly lower than the conventional group[(7.8 ±1.1) mg/L vs (16.2 ± 3.0) mg/L,(71.3 ±15.2) ng/L vs (102.4 ±17.8) ng/L,(2.6 ±0.3) μg/L vs (3.1 ±0.4) μg/L, (6.1 ±0.7) ×109/L vs (7.6 ±0.9) ×109/L,(2.0 ±0.5) ×109/L vs (2.8 ±0.6) ×109/L,(1.2 ± 0.3) μg/L vs (2.0 ±0.4) μg/L,(40.2 ±4.1) mmHg vs (48.3 ±3.6) mmHg,P<0.01],while B cells, NK cells,PaO2 were significantly higher than the conventional group[(36.7 ±2.8)% vs (32.9 ±2.5) %, (58.3 ±7.6)% vs(48.9 ±5.1)%,(72.8 ±6.2) mmHg vs (66.5 ±7.1) mmHg,P<0.01].Conclusion N-acetylcysteine is an effective drug in treatment of community acquired pneumonia ,which can significantly improve immune function and levels of inflammatory cytokines ,which can also significantly

  18. Potencialização do efeito metemoglobinizante da dapsona em ratos pela N-acetilcisteína Potentiation of dapsone induced methemoglobinemia by N-acetylcysteine in rats

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    Natália Valadares de Moraes


    Full Text Available Dapsona (DDS (4,4'diaminodifenilsulfona, fármaco de escolha para o tratamento da hanseníase, freqüentemente induz anemia hemolítica e metemoglobinemia. A N-hidroxilação, uma de suas principais vias de biotransformação, é constantemente relacionada com a metemoglobinemia observada com o uso do fármaco. Com o objetivo de prevenir a hemotoxicidade induzida pela DDS, N-acetilcisteína, fármaco precursor de glutationa, foi administrada em associação com DDS em ratos machos Wistar pesando 220-240 g. Os animais foram anestesiados e o sangue coletado da aorta para determinação da concentração plasmática de DDS por CLAE, determinação dos níveis de metemoglobina e de glutationa eritrocitária por espectrofotometria, e avaliação de parâmetros bioquímicos e hematológicos. Os resultados obtidos mostraram que a N-acetilcisteína potenciou o efeito metemoglobinizante da dapsona devido ao aumento de sua concentração plasmática e conseqüente aumento da formação da N-hidroxilamina. Concluímos que as interações medicamentosas com a dapsona exigem estudos individualizados a fim de evitar os efeitos adversos do fármaco.Dapsone (DDS (4,4'diaminodiphenylsulfone, the drug of choice for the treatment of leprosy, frequently induces hemolytic anemia and methemoglobinemia. N-hydroxylation, one of the major pathways of biotransformation, has been constantly related to the methemoglobinemia after the use of the drug. In order to prevent the dapsone-induced hemotoxicity, N-acetylcysteine, a drug precursor of glutathione, was administered in combination with DDS to male Wistar rats, weighting 220-240 g. The animals were then anaesthetized and blood was collected from the aorta for determination of plasma DDS concentration by HPLC, determination of methemoglobinemia and glutathione by spectrophotometry, and for biochemical and hematological parameters. Our results showed that N-acetylcysteine enhanced dapsone-induced methemoglobinemia due to

  19. Protective influences of N-acetylcysteine against alcohol abstinence-induced depression by regulating biochemical and GRIN2A, GRIN2B gene expression of NMDA receptor signaling pathway in rats. (United States)

    Yawalkar, Rutuja; Changotra, Harish; Gupta, Girdhari Lal


    Evidences have indicated a high degree of comorbidity of alcoholism and depression. N-acetylcysteine (NAC) has shown its clinical efficiency in the treatment of several psychiatric disorders and is identified as a multi-target acting drug. The ability of NAC to prevent alcohol abstinence-induced depression-like effects and underlying mechanism(s) have not been adequately addressed. This study was aimed to investigate the beneficial effects of NAC in the alcohol abstinence-induced depression developed following long-term voluntary alcohol intake. For evaluation of the effects of NAC, Sprague-Dawley rats were enabled to voluntary drinking of 4.5%, 7.5% and 9% v/v alcohol for fifteen days. NAC (25, 50, and 100 mg/kg) and fluoxetine (5 mg/kg) were injected intraperitoneally for three consecutive days during the alcohol abstinence period on the days 16, 17, 18. The behavioral studies were conducted employing forced swim test (FST), and tail suspension test (TST) on day 18 to determine the effects of N-acetylcysteine and fluoxetine in the ethanol withdrawal induced-depression. Blood alcohol concentration, alcohol biomarkers like SGPT, SGOT, ALP, GGT, and MCV were estimated by using commercially available kits. Serotonin concentrations were measured in the plasma, hippocampus and pre-frontal cortex using the rat ELISA kit. The expression of GRIN1, GRIN2A, GRIN2B genes for the N-methyl d-aspartate receptors (NMDAR) subunits in the hippocampus and the prefrontal cortex were also examined by reverse-transcription quantitative polymerase chain reaction. The results revealed that alcohol abstinence group depicted increased immobility time in FST and TST. Further, NAC exerted significant protective effect at the doses 50 mg/kg and 100 mg/kg, but 25 mg/kg showed insignificant protection against alcohol abstinence-induced depression. The increased level of biochemical parameters following ethanol abstinence were also reversed by NAC at the dose of 100 mg/kg. The

  20. The add-on N-acetylcysteine is more effective than dimethicone alone to eliminate mucus during narrow-band imaging endoscopy: a double-blind, randomized controlled trial. (United States)

    Chen, Ming-Jen; Wang, Horng-Yuan; Chang, Chen-Wang; Hu, Kuang-Chun; Hung, Chien-Yuan; Chen, Chih-Jen; Shih, Shou-Chuan


    Recent studies have shown that pronase can improve mucosal visibility, but this agent is not uniformly available for human use worldwide. This study aimed to assess the efficacy of N-acetylcysteine (NAC), a mucolytic agent, in improving mucus elimination as measured by decreased endoscopic water flushes during narrow-band imaging (NBI) endoscopy. A consecutive series of patients scheduled for upper gastrointestinal endoscopy at outpatient clinics were enrolled in this double-blind, randomized controlled trial. The control group drank a preparation of 100 mg dimethicone (5 ml at 20 mg/ml) plus water up to 100 ml, and the NAC group drank 300 mg NAC plus 100 mg dimethicone and water up to 100 ml. During the endoscopy, the endoscopist used as many flushes of water as deemed necessary to produce a satisfactory NBI view of the entire gastric mucosa. In all, 177 patients with a mean age of 51 years were evaluated in this study. Significantly lesser water was used for flushing during NBI endoscopy for the NAC group than the control group; 40 ml (30-70, 0-120) versus 50 ml (30-100, 0-150) (median (interquartile range, range), p = 0.0095). Considering the safety profile of NAC, decreasing the number of water flushes for optimal vision and unavailability of pronase in some areas, the authors suggest the use of add-on NAC to eliminate mucus during NBI endoscopy.

  1. Nephroprotective Effects of N-Acetylcysteine Amide against Contrast-Induced Nephropathy through Upregulating Thioredoxin-1, Inhibiting ASK1/p38MAPK Pathway, and Suppressing Oxidative Stress and Apoptosis in Rats

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    Xuezhong Gong


    Full Text Available Contrast-induced nephropathy (CIN is a leading cause of hospital-acquired acute kidney injury (AKI due to apoptosis induced in renal tubular cells. Our previous study demonstrated the novel N-acetylcysteine amide (NACA; the amide form of N-acetyl cysteine (NAC prevented renal tubular cells from contrast-induced apoptosis through inhibiting p38 MAPK pathway in vitro. In the present study, we aimed to compare the efficacies of NACA and NAC in preventing CIN in a well-established rat model and investigate whether thioredoxin-1 (Trx1 and apoptosis signal-regulating kinase 1 (ASK1 act as the potential activator for p38 MAPK. NACA significantly attenuated elevations of serum creatinine, blood urea nitrogen, and biomarkers of AKI. At equimolar concentration, NACA was more effective than NAC in reducing histological changes of renal tubular injuries. NACA attenuated activation of p38 MAPK signal, reduced oxidative stress, and diminished apoptosis. Furthermore, we demonstrated that contrast exposure resulted in Trx1 downregulation and increased ASK1/p38 MAPK phosphorylation, which could be reversed by NACA and NAC. To our knowledge, this is the first report that Trx1 and ASK1 are involved in CIN. Our study highlights a renal protective role of NACA against CIN through modulating Trx1 and ASK1/p38 MAPK pathway to result in the inhibition of apoptosis among renal cells.

  2. Development and utilization of extracorporeal regional complexing hemodialysis as a means of mobilizing and enhancing the excretion of methylmercury in the dog. [N-acetylcysteine; N-acetylpenicillamine; 2,3-dimercaptosuccinic acid

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    Kostyniak, P.J.


    The present investigation was directed at developing and testing a new procedure for increasing methylmercury excretion in the dog. The procedure utilizes hemodialysis in conjunction with the extracorporeal reversal of protein binding of methylmercury in blood by the presence of low molecular weight sulfhydryl containing complexing agents (cysteine, N-acetylcysteine, penicillamine, N-acetylpenicillamine, 2,3-dimercaptosuccinic acid) having a high chemical affinity for methylmercury. Using such a procedure, the complexed methylmercury and the free complexing agent were found to be readily removed from blood by the dialyzer. Unlike chelation therapy, this procedure does not rely on the attainment of high systemic concentrations of complexing agent in order to attain enhanced excretion by normal routes. It rather introduces into the circulatory system a shunt designed specifically for methylmercury extraction from blood. In vitro testing of this procedure revealed that methylmercury removal from blood was dependent upon the concentration of complexing agent in blood and the dialyzer blood flow rate. In vivo testing of the procedure in the dog utilized a standard hemodialyzer with infusion of complexing agent into the arterial dialyzer blood line. The rate of methylmercury removal from the dog during the treatment procedures were as high as 400 times the excretion rate of mercury in untreated dogs.

  3. Add-on treatment with N-acetylcysteine for bipolar depression: a 24-week randomized double-blind parallel group placebo-controlled multicentre trial (NACOS-study protocol). (United States)

    Ellegaard, Pernille Kempel; Licht, Rasmus Wentzer; Poulsen, Henrik Enghusen; Nielsen, René Ernst; Berk, Michael; Dean, Olivia May; Mohebbi, Mohammadreza; Nielsen, Connie Thuroee


    Oxidative stress and inflammation may be involved in the development and progression of mood disorders, including bipolar disorder. Currently, there is a scarcity of useful treatment options for bipolar depressive episodes, especially compared with the efficacy of treatment for acute mania. N-Acetylcysteine (NAC) has been explored for psychiatric disorders for some time given its antioxidant and anti-inflammatory properties. The current trial aims at testing the clinical effects of adjunctive NAC treatment (compared to placebo) for bipolar depression. We will also explore the biological effects of NAC in this context. We hypothesize that adjunctive NAC treatment will reduce symptoms of depression, which will be reflected by changes in selected markers of oxidative stress. In the study, we will include adults diagnosed with bipolar disorder, in a currently depressive episode. Participants will undertake a 20-week, adjunctive, randomized, double-blinded, parallel group placebo-controlled trial comparing 3 grams of adjunctive NAC daily with placebo. The primary outcome is the mean change over time from baseline to end of study on the Montgomery-Asberg Depression Rating Scale (MADRS). Among the secondary outcomes are mean changes from baseline to end of study on the Bech-Rafaelsen Melancholia Scale (MES), the Young Mania Rating Scale (YMRS), the WHO-Five Well-being Index (WHO-5), the Global Assessment of Functioning scale (GAF-F), the Global Assessment of Symptoms scale (GAF-S) and the Clinical Global Impression-Severity scale (CGI-S). The potential effects on oxidative stress by NAC treatment will be measured through urine and blood samples. DNA will be examined for potential polymorphisms related to oxidative defences. Registered at The European Clinical Trials Database, NCT02294591 and The Danish Data Protection Agency: 2008-58-0035.

  4. The efficacy of N-acetylcysteine plus sodium bicarbonate in the prevention of contrast-induced nephropathy after cardiac catheterization and percutaneous coronary intervention: A meta-analysis of randomized controlled trials. (United States)

    Zhao, Shi-Jie; Zhong, Zhao-Shuang; Qi, Guo-Xian; Tian, Wen


    The efficacy of combining use of N-acetylcysteine (NAC) and sodium bicarbonate (SOB) in the prevention of contrast-induced nephropathy (CIN) after cardiac catheterization and percutaneous coronary intervention (PCI) is unclear. All relevant studies that compared the effect of combining the use of NAC and SOB with individual use on CIN in patients undergoing cardiac catheterization and PCI were identified by searching the databases including Pubmed, Embase, Cochrane Library, and Web of Science without time and language limitation. Only randomized controlled trials (RCTs) with full-text published were considered. Sixteen RCTs involving 4432 cases were included into this meta-analysis. The results showed there were no additional benefit in reduction of CIN in COM group (COM versus NAC: RR 0.85, 95% CI 0.70-1.03, P=0.103; COM versus SOB: RR 0.91, 95% CI 0.71-1.16, P=0.449), even in patients with diabetes mellitus (COM versus NAC: RR 1.11, 95% CI 0.71-1.75, P=0.646; COM versus SOB: RR 1.06, 95% CI 0.45-2.47, P=0.893), undergoing PCI procedure (COM versus NAC: RR0.76, 95% CI 0.39-1.47, P=0.411; COM versus SOB: RR0.96, 95% CI 0.65-1.40, P=0.814), or with baseline renal dysfunction (COM versus NAC: RR 0.89, 95% CI 0.70-1.14, P=0.366; COM versus SOB: RR 0.95, 95% CI 0.67-1.36, P=0.788). The present study demonstrated combining use of NAC and SOB was not significantly superior to individual use method in the prevention of CIN after cardiac catheterization and PCI. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  5. Schlafmedizinische Charakterisierung von Parkinson-Patienten mit Schlafattacken unter dopaminerger Therapie


    Rethfeldt, Mira


    1999 wurden erstmals sogenannte Schlafattacken bei Parkinson-Patienten unter der Therapie mit Nonergolin-Dopaminagonisten berichtet. Später zeigten Studien, dass diese Schlafattacken unter jeglicher dopaminerger Therapie auftreten können. Bis heute ist jedoch die Pathophysiologie dieses Phänomens nicht hinreichend geklärt. Es wird diskutiert, ob diese Attacken als paroxysmales Symptom überhaupt bestehen oder nicht vielmehr ...

  6. Acrylamide-induced oxidative stress and inflammatory response are alleviated by N-acetylcysteine in PC12 cells: Involvement of the crosstalk between Nrf2 and NF-κB pathways regulated by MAPKs. (United States)

    Pan, Xiaoqi; Wu, Xu; Yan, Dandan; Peng, Cheng; Rao, Chaolong; Yan, Hong


    Acrylamide (ACR) is a classic neurotoxin in animals and humans. However, the mechanism underlying ACR neurotoxicity remains controversial, and effective prevention and treatment measures against this condition are scarce. This study focused on clarifying the crosstalk between the involved signaling pathways in ACR-induced oxidative stress and inflammatory response and investigating the protective effect of antioxidant N-acetylcysteine (NAC) against ACR in PC12 cells. Results revealed that ACR exposure led to oxidative stress characterized by significant increase in reactive oxygen species (ROS) and malondialdehyde (MDA) levels and glutathione (GSH) consumption. Inflammatory response was observed based on the dose-dependently increased levels of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6). NAC attenuated ACR-induced enhancement of MDA and ROS levels and TNF-α generation. In addition, ACR activated nuclear transcription factor E2-related factor 2 (Nrf2) and nuclear factor-κB (NF-κB) signaling pathways. Knockdown of Nrf2 by siRNA significantly blocked the increased NF-κB p65 protein expression in ACR-treated PC12 cells. Down-regulation of NF-κB by specific inhibitor BAY11-7082 similarly reduced ACR-induced increase in Nrf2 protein expression. NAC treatment increased Nrf2 expression and suppressed NF-κB p65 expression to ameliorate oxidative stress and inflammatory response caused by ACR. Further results showed that mitogen-activated protein kinases (MAPKs) pathway was activated prior to the activation of Nrf2 and NF-κB pathways. Inhibition of MAPKs blocked Nrf2 and NF-κB pathways. Collectively, ACR activated Nrf2 and NF-κB pathways which were regulated by MAPKs. A crosstalk between Nrf2 and NF-κB pathways existed in ACR-induced cell damage. NAC protected against oxidative damage and inflammatory response induced by ACR by activating Nrf2 and inhibiting NF-κB pathways in PC12 cells. Copyright © 2018 Elsevier B

  7. A randomized controlled trial comparing hydration therapy to additional hemodialysis or N-acetylcysteine for the prevention of contrast medium-induced nephropathy: the Dialysis-versus-Diuresis (DVD) Trial. (United States)

    Reinecke, H; Fobker, M; Wellmann, J; Becke, B; Fleiter, J; Heitmeyer, C; Breithardt, G; Hense, H-W; Schaefer, R M


    Contrast medium-induced nephropathy (CIN) is a serious complication with increasing frequency and an unfavorable prognosis. Previous analyses of surrogate parameters have suggested beneficial effects of hemodialysis that are assessed in this randomized clinical trial. We performed a prospective single-center trial in 424 consecutive patients with serum creatinine concentrations between 1.3- 3.5 mg/dl who underwent elective coronary angiography. Patients were randomized to one of three treatment strategies with all patients receiving pre- and postprocedural hydration: One group received no additional therapy, patients in the second group were hemodialyzed once, and the third group received oral N-acetylcysteine. The frequency of CIN (defined as an increase in serum creatinine>or=0.5 mg/dl) from 48 to 72 h after catheterization was 6.1% in the hydration-only group, 15.9% with hemodialysis treatment, and 5.3% in the N-ACC group (intention-to-treat analysis; P=0.008). There were no differences between the treatment groups with regard to increased (>or=0.5 mg/dl) serum creatinine concentrations after 30-60 days (4.8%, 5.1%, and 3.1%, respectively; P=0.700). Analyses of long-term follow-up (range 63 to 1316 days) by Cox regressions models of the study groups found quite similar survival rates (P=0.500). In contrast to other (retrospective) studies, long-term survival of patients with vs those without CIN within 72 h was not different, but patients who still had elevated creatinine concentrations at 30-60 days suffered from a markedly higher 2-year mortality (46% vs 17%, P=0.002). In conclusion, hemodialysis in addition to hydration therapy for the prevention of CIN provided no evidence for any outcome benefit but evidence for probable harm. Increased creatinine concentrations at 30-60 days, but not within 72 h, were associated with markedly reduced long-term survival.

  8. N-acetylcysteine for major depressive episodes in bipolar disorder N-acetilcisteína para o tratamento de episódios de depressão maior no transtorno bipolar

    Directory of Open Access Journals (Sweden)

    Pedro V Magalhães


    Full Text Available OBJECTIVE: In this report, we aimed to evaluate the effect of add-on N-acetylcysteine (NAC on depressive symptoms and functional outcomes in bipolar disorder. To that end, we conducted a secondary analysis of all patients meeting full criteria for a depressive episode in a placebo controlled trial of adjunctive NAC for bipolar disorder. METHOD: Twenty-four week randomised clinical trial comparing adjunctive NAC and placebo in individuals with bipolar disorder experiencing major depressive episodes. Symptomatic and functional outcome data were collected over the study period. RESULTS: Seventeen participants were available for this report. Very large effect sizes in favor of NAC were found for depressive symptoms and functional outcomes at endpoint. Eight of the ten participants on NAC had a treatment response at endpoint; the same was true for only one of the seven participants allocated to placebo. DISCUSSION: These results indicate that adjunctive NAC may be useful for major depressive episodes in bipolar disorder. Further studies designed to confirm this hypothesis are necessary.OBJETIVO: Neste relato, avaliamos o efeito da N-acetilcisteína (NAC adjuvante em sintomas depressivos e desfechos funcionais no transtorno bipolar. Para isso, conduzimos uma análise secundária de todos os pacientes com critérios diagnósticos para um episódio depressivo em um ensaio clínico randomizado comparando NAC adjuvante com placebo no transtorno bipolar. MÉTODO: Ensaio clínico randomizado comparando NAC adjuvante com placebo para episódios depressivos no transtorno bipolar durante 24 semanas. Desfechos funcionais e sintomáticos foram coletados no período. RESULTADOS: Dezessete participantes estavam disponíveis para esta análise. Tamanhos de efeito grandes foram encontrados para sintomas depressivos e desfechos funcionais. Oito dos dez participantes no grupo da NAC tiveram resposta clínica ao fim do tratamento. O mesmo ocorreu em apenas um dos sete

  9. N-Acetylcysteine Reverses Cocaine Induced Metaplasticity


    Moussawi, Khaled; Pacchioni, Alejandra; Moran, Megan; Olive, M. Foster; Gass, Justin T.; Lavin, Antonieta; Kalivas, Peter W


    Cocaine addiction is characterized by an impaired ability to develop adaptive behaviors that can compete with cocaine seeking, implying a deficit in the ability to induce plasticity in cortico-accumbens circuitry critical for regulating motivated behavior. RWe found that rats withdrawn from cocaine self-administration had a marked in vivo deficit in the ability to develop long-term potentation (LTP) and depression (LTD) in the nucleus accumbens core subregion following stimulation of prefront...

  10. Nefropatia induzida por contraste: avaliação da proteção pela n-acetilcisteína e alopurinol em ratos uninefrectomizados Contrast-induced nephropathy: evaluation of n-acetylcysteine and allopunirol protective effect in uninephrectomized rats

    Directory of Open Access Journals (Sweden)

    José Carlos Carraro Eduardo


    Full Text Available OBJETIVO: A nefropatia por contraste é a terceira causa de insuficiência renal aguda em pacientes hospitalizados. O objetivo deste estudo foi avaliar a ação da n-acetilcisteína e do alopurinol na proteção renal em ratos de ambos os sexos que receberam diatrizoato. MATERIAIS E MÉTODOS: Ratos Wistar adultos jovens, uninefrectomizados e submetidos a restrição hídrica, receberam solução salina (grupo 1: machos; grupo 2: fêmeas, diatrizoato (grupo 3: machos; grupo 4: fêmeas, diatrizoato e n-acetilcisteína (grupo 5: machos, diatrizoato e alopurinol (grupo 6: machos e diatrizoato e n-acetilcisteína + alopurinol (grupo 7: machos. A filtração glomerular foi avaliada pela creatinina. O teste t de Student e o teste do sinal foram utilizados para análises estatísticas. RESULTADOS: Ratos que receberam diatrizoato apresentaram elevação estatisticamente significante da creatinina sérica, quando comparados aos controles, porém não houve diferença entre os sexos. Os animais que receberam alopurinol não mostraram aumento significante da creatinina, enquanto a administração de n-acetilcisteína não impediu a elevação da creatinina. CONCLUSÃO: O alopurinol mostrou-se mais efetivo que a n-acetilcisteína na proteção funcional renal ao dano induzido pelo diatrizoato de sódio. Não houve diferença entre os sexos na intensidade do dano renal pelo diatrizoato de sódio.OBJECTIVE: Contrast medium-induced nephropathy is the third most frequent cause of iatrogenic acute renal failure involving inpatients. The present study was aimed at evaluating the protective effect of n-acetylcysteine and allopurinol in both male and female rats receiving diatrizoate. MATERIALS AND METHODS: Thirty-five young adult Wistar rats submitted to hydric restriction were divided into groups as follows: groups 1 and 2 (respectively male and female rats receiving saline solution; groups 3 and 4 (respectively male and female rats receiving diatrizoate; group 5

  11. N-acetylcysteine and fructose-1,6-bisphosphate: immunomodulatory effects on mononuclear cell culture N-acetilcisteína e frutose-1,6-bisfosfato: efeito imunomodulador em cultura de células mononucleares

    Directory of Open Access Journals (Sweden)

    Ricardo Obalski de Mello


    Full Text Available INTRODUCTION: Sepsis is a complex syndrome caused by an uncontrolled systemic inflammatory response. Inflammatory cytokines play a pivotal role in septic shock pathogenesis. Therapeutic strategies have been tested in order to modulate the excessive generation or function of sepsis mediators. OBJECTIVE: The objective of the present study was to investigate the therapeutic effect of N-acetylcysteine (NAC and its association with fructose-1,6-bisphosphate (FBP on T-lymphocytes proliferation, interleukin-1β (IL-1β and monocyte chemotactic protein-1 (MCP-1 levels. MATERIAL AND METHODS: Peripheral blood mononuclear cell samples were isolated from healthy individuals. T-lymphocytes were stimulated with phytohemagglutinin for 96 hours and submitted to different concentrations of NAC or NAC associated with FBP. RESULTS: NAC (10 and 15 mM and NAC (15 mM associated with FBP reduced T-lymphocytes proliferation. IL-1β levels rose in the presence of both NAC (15 mM and NAC with FBP (1.25 mM. MCP-1 levels were reduced only by NAC (15 mM associated with FBP (1.25 mM. CONCLUSION: The results suggest that both NAC itself and NAC associated with FBP inhibit cellular proliferation, acting as potent immunomodulatory agents, which corroborates its use in the treatment of inflammatory diseases.INTRODUÇÃO: A sepse é uma síndrome complexa causada pela resposta inflamatória sistêmica descontrolada. As citocinas inflamatórias representam papel central na patogênese do choque séptico. Têm sido testadas estratégias terapêuticas a fim de modular a geração ou a função excessiva de mediadores na sepse. OBJETIVO: O objetivo deste estudo foi investigar o efeito terapêutico da N-acetilcisteína (NAC e sua associação com a frutose-1,6-bisfosfato (FBP sobre a proliferação de linfócitos T e a geração de interleucina-1β (IL-1β e proteína quimiotática de monócitos 1 (MCP-1 em cultura celular. MATERIAL E MÉTODOS: Foram isoladas células mononucleares de

  12. Efeitos da N-acetilcisteína no precondicionamento isquêmico: estudo em corações isolados de ratos Effects of n-acetylcysteine on ischemic preconditioning: study in isolated rat hearts

    Directory of Open Access Journals (Sweden)

    Denoel Marcelino de Oliveira


    Full Text Available OBJETIVO: Avaliar se a N-Acetilcisteína (NAC altera o Precondicionamento Isquêmico (PC em corações isolados de ratos usando apenas um ciclo de PC. MÉTODOS: Freqüência Cardíaca (FC, Fluxo Coronariano (FLC e Contratilidade Miocárdica (dP/dt foram registradas em 30 corações de ratos Wistar. Após anestesia, os corações foram perfundidos em sistema de Langendorff com solução de Krebs-Hensleit (K-H, equilibrada (95% de O2 e 5% de CO2. GI: Controle (n=6; GII: 20 min. isquemia (n=6; GIII: PC (n=6; GIV 50 µg/ml/min NAC antes do PC (n =6; GV: 100 µg/ml/min NAC antes do PC (n=6. Todos os parâmetros foram mensurados após 15 minutos de estabilização (T0 e T3, T5, T10, T15, T20, T25 e T30 minutos de reperfusão. Significância estatística foi considerada quando P0,05. dP/dt foi maior no GV comparado com GIV, mas com diferença estatisticamente significativa somente em T30. CONCLUSÃO: Os corações precondicionados tiveram melhor dP/dt, sendo alteradas pelo uso de NAC no GIV e não alteradas no GV.OBJECTIVE: The aim of this study is to assess if N-Acetylcysteine (NAC changes the Ischemic Preconditioning (IP in isolated rat hearts using only one cycle of IP. METHODS: Heart Rate (HR, Coronary Flow (CF and Myocardial Contractility (dP/dt were registered in 30 Wistar rat's hearts. After anesthesia the hearts were removed and perfused with Krebes-Hensleit equilibrated solution with 95% of O2 and 5% of CO2 according Langendorff's method. GI: Control (n=6; GII: 20 min. ischemia (n=6; GIII: IP (n=6; GIV 50 µg/ml/min NAC before IP (n =6; GV: 100 µg/ ml/min NAC before IP (n=6. Parameters were measured after 15 min. of stabilization (T 0 and T3, T5, T10, T15, T20, T25 and T30 min. after reperfusion. Statistical significance was considered when P0.05. dP/dt was higher in GV than GIV but with statistically significant difference only at T30. CONCLUSION: dP/dt was better in preconditioned hearts and was changed if using NAC in GIV. The use of NAC

  13. Labelling, biodistribution and compartmental analysis of N-acetylcysteine labelled with Tc-99m. Comparative investigation with with {sup 99m} Tc-MIBI in an in vivo tumoral model; Estudo de marcacao, biodistribuicao e analise compartimental da N-acetil cisteina marcada com Tc-99m. Investigacao comparativa com MIBI-{sup 99m}Tc em modelo tumoral in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Faintuch, Bluma Linkowski


    Labelling and biodistribution studies were done with two different ligands, respectively Methoxy isobutyl isonitrile (MIBI) and N-acetylcysteine (NAC), employing Tc-99m as a tracer. The main objective was to assess the pharmacokinetic properties of the second substance, aiming at its possible application in cancer diagnosis. To this purpose an in vivo investigation was done using healthy and tumor-bearing rats with experimental cancer. Images of tumor-bearing rats registered in a scintillation camera indicated that with {sup 99m} Tc-MIBI none of the two selected times was adequate for visualization of the cancer mass. In contrast, {sup 99m} Tc-NAC permitted clear identification of the humor, four hours after injection. The results have demonstrated that {sup 99m} Tc-NAC is a radiopharmaceutical with affinity for cancer tissue and promising for further investigation concerning imaging diagnosis of tumors. (author)

  14. Messung der Respirationsverschiebung intraabdominaler Organe am Beispiel der Leber unter maschineller Ventilation


    Olbrich, Bernhard


    Ziel dieser Arbeit ist es, die Bewegung intraabominaler Organe am Beispiel der Leber unter den Bedingungen maschineller Beatmung zu quantifizieren. Ein Messverfahren mit Hilfe eines elektromagnetischen Trackingsystems, wurde hierzu im Tierversuch neu entwickelt. Im Anschluss wurden die Messdaten mit Hilfe einer Explorativen Datenanalyse, validiert und ein einfaches Modell entwickelt, das die virtuelle Kompensation der Atemverschiebung ermöglicht. Zusätzlich wird eine praktische Anwendungsmögl...

  15. Die Analyse der Wertschöpfungskette Schweinefleisch unter den Aspekten des Supply Chain Management


    Schönberger, Wolfgang


    Inhalt der Arbeit ist die vergleichende Analyse der Wertschöpfungskette Schweinefleisch unter den Aspekten des Supply Chain Management für Bayern sowie für Dänemark als dem weltweit größten Nettoexporteur von Schweinefleisch. Wichtige Wettbewerbsfaktoren und die Strukturen der Wertschöpfungskette werden untersucht. Die Produktionskosten werden nach der Methode der Vollkostenrechnung ermittelt. In Dänemark haben sich auf allen Stufen der Wertschöpfungskette Strukturen entwickelt, die die...

  16. Investigation of LPP combustors under elevated pressure conditions; Untersuchungen zu LPP-Flugtriebwerksbrennkammern unter erhoehtem Druck

    Energy Technology Data Exchange (ETDEWEB)

    Fink, R.


    The development of new combustor concepts for aero engines to meet future emissions regulations in based on a detailed knowledge of the combustion process and the velocity field. In the presented thesis, non intrusive measurements were performed in a model combustion chamber under almost realistic pressure and temperature conditions. The species OH, NO, unburned hydrocarbons and fuel droplets were detected in 2 dimensions with the Laser Induced Fluorescence (LIF). The velocity field was measured with the Particle Image Velocimetry technique (PIV). [German] Die Weiterentwicklung neuer Brennkammerkonzepte zur Erfuellung zukuenftiger Schadstoffemissionsrichtlinien erfordert genaue Kenntnisse der ablaufenden Verbrennungs- und Stroemungsvorgaenge in der Brennkammer. Bei den in der Arbeit vorgestellten Untersuchungen wurden in einer LPP-Modellbrennkammer unter annaehernd realistischen Eintrittsbedingungen die Spezies OH, NO, unverbrannte Kohlenwasserstoffe sowie noch fluessiger Brennstoff zweidimensional anhand der Laserinduzierten Fluoreszenz (LIF) nachgewiesen. Das Stroemungsfeld wurde mit Hilfe der Particle Image Velocimetry (PIV) gemessen.

  17. Pharmacodynamic Effect of N-Acetylcysteine as Adjunctive Therapy ...

    African Journals Online (AJOL)


    Tropical Journal of Pharmaceutical Research April 2011; 10 (2): 141-146 ... Immulogy and Rheumatology, Department of Medicine, Faculty of Medicine, Ramathibodi ... received standard treatment for mild SLE; ..... Arthritis Rheum 2005;.

  18. Bivirkninger ved N-acetylcystein-behandling af paracetamolforgiftede patienter

    DEFF Research Database (Denmark)

    Schmidt, L E; Dalhoff, K P


    number of patients will be treated with NAC than with previous regimens based on plasma concentrations of paracetamol. In this retrospective study we evaluated the incidence of side effects of NAC in 310 patients admitted to the Department of Hepatology, Rigshospitalet, Copenhagen, over a four-year...... period (1.1.1994-31.12.1997). Twenty-six (8.4%) patients developed side effects. Side effects were anaphylactoid, mainly from skin (25 rash, pruritus or flushing), in rare cases more serious (four bronchospasm, three angioedema, one hypotension). None were life-threatening and all patients received...

  19. Anforderungen an Marketingkonzepte für Großtraktoren unter Berücksichtigung des Wandels landwirtschaftlicher Rahmenbedingungen


    Thiel, Jörg


    Beeinflusst von der Volatilität der Märkte, ergänzt um gesellschaftspolitische wie absatzorientierte Maßnahmen, ist die Landtechnikbranche seit jeher einem permanenten Wandel ausgesetzt. Insbesondere unter dem derzeit im Fokus stehenden Aspekt der Nachhaltigkeit in landwirtschaftlichen Produktionssystemen bedarf es der Gestaltung innovativer Ansätze für effizientere sowie gleichzeitig ressourcenschonende Verfahren. Zusätzlich verstärkt durch die seit geraumer Zeit anhaltend angespannten Situa...

  20. Fragmentierungsarten von Nierensteinen unter extrakorporaler Stoßwellenlithotripsie: eine multivariate Analyse

    Directory of Open Access Journals (Sweden)

    Aeberli D


    Full Text Available Das primäre Ziel dieser retrospektiven Studie war die Evaluation von routinemäßig anwendbaren Kriterien, welche die Fragmentierung von Nierensteinen unter extrakorporaler Stoßwellenlithotripsie (ESWL voraussagen können. Die Serie umfaßte 202 konsekutive Patienten (121 Männer, 81 Frauen mit einem medianen Alter von 48 Jahren (19-81 Jahre, die an der Urologischen Universitätsklinik Bern mit dem originalen Dornier HM-3 behandelt wurden. Einschlußkriterien waren Einzelsteine von 10-30 mm Größe mit Lage im Nierenbeckenkelchsystem. Die Steine wurden im Abdomen-Leerbild nach ihrer Lage, Größe, Form, Dichte (im Vergleich zur 12. Rippe, Struktur und Oberfläche beurteilt. Weiter wurden Alter, Geschlecht und Body-Mass-Index des Patienten berücksichtigt. Die Desintegration wurde am 1. Tag nach ESWL mittels Abdomen-Leerbild dokumentiert. Zielwert der Analyse war die binäre Variable steinfrei versus Restfragmente, gegen die alle präoperativen Parameter mittels multivariater logistischer Regression getestet wurden. Die Erfolgsrate betrug 95,5 %; 42 Patienten (20,8 % waren steinfrei und 151 Patienten (74,7 % hatten sog. klinisch insignifikante Restfragmente (5 mm oder kleiner. Von den Männern waren 14,9 %, von den Frauen 29,6 % steinfrei (p = 0,01. Alle anderen Parameter erreichten keine statistische Signifikanz, möglicherweise aufgrund zu kleiner Subgruppen (fehlende statistische Macht. Die Desintegrationsrate des HM-3 für Nierensteine ist insgesamt ausgezeichnet, bei Frauen noch besser als bei Männern. Gerade wegen dieser hohen Erfolgsrate wären größere Patientenzahlen notwendig, um eventuelle Unterschiede zwischen den präinterventionellen Parametern definieren zu können.

  1. "America first": die Außen- und Sicherheitspolitik der USA unter Präsident Trump



    "Donald J. Trump ist der 45. Präsident der Vereinigten Staaten - und auch zehn Wochen nach der Wahl hat sich die Aufregung über das Wahlergebnis nicht gelegt. Diesseits des Atlantiks interessiert vor allem eine Frage: Wie wird die amerikanische Außen- und Sicherheitspolitik unter Präsident Trump aussehen? Wird sie von Kontinuität geprägt sein? Oder wird es gravierende Brüche geben? In diesem Report werfen HSFK-Autorinnen und -Autoren Blicke auf Themen, die aus Sicht der Friedens- und Konflikt...

  2. Rigiscan®-Monitoring der Erektion unter audiovisueller sexueller Stimulation ohne/mit Viagra™ bei Patienten mit erektiler Dysfunktion

    Directory of Open Access Journals (Sweden)

    Perabo FGE


    Full Text Available Die orale Medikation mit Sildenafil (Viagra™ hat sich zur Therapie der erektilen Dysfunktion etabliert und relativiert die Differentialdiagnose zwischen psychogener und organischer Dysfunktion mit den entsprechenden therapeutischen Konsequenzen. Diesem Problem widmet sich diese Studie zur Prüfung der erektilen Antwort unter RigiScan®-Monitoring ohne/mit 50 mg Viagra™. 64 Patienten (mittleres Alter 48 Jahre mit einer erektilen Dysfunktion arteriogener (n = 10, venöser (n = 7, gemischt neurogen/vaskulärer (n = 15 und psychogener (n = 32 Genese von mehr als 6 Monaten Dauer wurden in die Studie aufgenommen. Alle Patienten wurden eingehend diagnostisch abgeklärt, inklusive Hormonlabor, dynamischer Pharmako-Duplexsonographie und, falls indiziert, mit einer Cavernosometrie/ -graphie. Zur Testung der penilen Rigidität und zur Objektivierung der Erektion wurde das "real-time" RigiScan® verwendet. In zwei konsekutiven Messungen wurden sowohl die direkte erektile Antwort auf visuelle Stimulation als auch die nächtlichen Erektionen abgeleitet und die Meßergebnisse mit den Begleiterkrankungen, "lifestyle"-Faktoren und dem Ergebnis der Pharmako-Duplexsonographie korreliert. Es fand sich keine Korrelation zwischen der Ätiologie der erektilen Dysfunktion in bezug auf organische oder psychogene Genese und dem Alter der Patienten, der Dauer der Erektionsstörung, dem Nikotinabusus, den Blutfetten, den Testosteronwerten, dem klinischen Ergebnis der Pharmakotestung (Erektionsgrad und dem duplexsonographisch gemessenen Fluß der penilen Arterien. Hingegen zeigte sich eine positive Korrelation zwischen Genese der erektilen Dysfunktion (organisch bzw. psychogen und dem RigiScan®-Meßergebnis (r = 0,29. Patienten mit psychogen bedingter Erektionsstörung profitierten am meisten von Viagra™, während Patienten mit vaskulärer Genese oder gemischt neurogener/vaskulärer Genese deutlich schlechter auf Viagra™ ansprachen. Es bestand keine Korrelation

  3. Zu entwicklungstendenzen im satzbau der Deutschen sprache der gegenwart unter besonderer Berücksichtigung der ausrahmung

    Directory of Open Access Journals (Sweden)

    Jasna Makovec


    Full Text Available Die vorliegende Arbeit wurde durch das Bestreben motiviert, weitere Ergänzungen zum Phänornen "Ausrahrnung in der modernen deutschen Schriftsprache der Gegenwart" zu leisten. Der Ausgangspunkt dieses "fakultativ" geregelten Bereiches ist die Annahme bzw. die Arbeitshypothese, daß sich linguistische und außerlinguistische Faktoren einander zuordnen lassen. Das bedeutet, daß die Linearität innerhalb des Kornrnunikationspro­ zesses auch einige wichtige kommunikative Funktionen auszuüben hat, und daß die kontextuellen Modifikationen der Stellungsva­ rianten bei gleichern Denotatsbezug unter diesern Blickwinkel untersucht werden müssen, und nicht gleich als stilistische Varianten qualifiziert werden.

  4. Mechanical-biological waste treatment with thermal processing of partial fractions; Mechanisch-biologische Restabfallbehandlung unter Einbindung thermischer Verfahren fuer Teilfraktionen

    Energy Technology Data Exchange (ETDEWEB)



    Technologies for mechanical-biological treatment of waste in the Land of Hessen were compared including thermal processes like combustion and gasification. The new and more rigid limiting values specified in the Technical Guide for Municipal Waste Treatment (Technische Anleitung Siedlungsabfall - TASI) get a special mention. [Deutsch] Verschiedene Technologien der mechanisch-biologischen Restabfallbehandlung im Raum Hessen wurden unter Einbezug thermischer Verfahren (Verbrennung, Vergasung) miteinander verglichen. Dabei wurden besonders auf die verschaerften Grenzwerte der Technischen Anleitung Siedlungsabfall (TASI) eingegangen. (ABI)

  5. Medienpädagogik unter der differenztheoretischen Lupe. Eine Identitätssuche zwischen Disziplin und Profession

    Directory of Open Access Journals (Sweden)

    Franziska Linke


    Full Text Available Der Beitrag diskutiert die dem Themenheft zugrundeliegende Fragestellung nach der Konstitution der Medienpädagogik aus einer systemtheoretischen Perspektive. Dabei wird im Anschluss an Stichweh (2013 Ansatz der funktionalen Differenzierung ein Untersuchungsrahmen aufgezeigt, der es ermöglicht, die Medienpädagogik sowohl aus wissenschaftssoziologischer als auch professionssoziologischer Perspektive zu betrachten. Gerade die zentrale Rolle der (digitalen Medien für Lern- und Bildungsprozesse sowie für den Arbeitsmarkt (u. a. KMK 2016 verlangt eine stetige Reflexion professioneller Handlungssysteme der Medienpädagogik, die nicht losgelöst von der Frage nach dem Selbstverständnis der Medienpädagogik als Wissenschaftsdisziplin gelingen kann (u. a. Hugger 2001. Divergierende theoretische Positionierungen der Akteurinnen und Akteure sowie verschiedene Zielvorstellungen zur Medienpädagogik selbst und zu dem, was sie praktisch leisten kann, erschweren ein solches Vorhaben. Die Autorinnen des Beitrags begeben sich daher, unter Berücksichtigung der Differenzierung zwischen Disziplin und Profession, auf die Suche nach möglichen Antworten. Vorgeschlagen wird eine erweiterte Betrachtungsweise, welche die Medienpädagogik als wissenschaftliche Produktionsgemeinschaft beschreibt und dadurch Potenziale eröffnet theoretische und empirische Erkenntnisse von Akteurinnen und Akteuren unterschiedlicher wissenschaftlicher Disziplinen mit Medienzusammenhang zu berücksichtigen. Ferner wird dargelegt, dass ein solcher Beschreibungsansatz neue Forschungsperspektiven auf die professionstheoretische Einordnung praktischer Handlungssysteme der Medienpädagogik eröffnen kann.

  6. Kontrastmittelverstärkte Magnet-Resonanz-Urographie unter forcierter Diurese - Stellenwert in der Differentialdiagnostik bei Obstruktionen des oberen Harntraktes

    Directory of Open Access Journals (Sweden)

    Jung P


    Full Text Available Die Magnet-Resonanz-Urographie (MRU ist ein relativ neues Verfahren in der Diagnostik des oberen Harntraktes. Das Ziel dieser Studie war der Vergleich der Aussagefähigkeit der MRU unter Gabe von Gadolinium und Furosemid und dem konventionellen Urogramm (IVU in der Diagnostik der Ursache von Obstruktionen im Bereich des Harnleiters. 82 Patienten mit im IVU nachgewiesener Obstruktion des oberen Harntraktes oder urographisch stummer Niere bei sonographisch nachgewiesener Dilatation wurden der MRU zugeführt. Die Bilder beider Untersuchungsmethoden wurden von voneinander unabhängigen Untersuchern befundet. Zwei Urologen befundeten die IVU-Bilder, zwei Radiologen die MRU-Bilder, die Radiologen kannten die IVU-Diagnose nicht. Bei unklarer Diagnose wurden weitere Untersuchungen wie Computertomographie, retrograde Pyelographie oder Ureteroskopie durchgeführt. Die Diagnosen waren: Harnleitersteine bei 72 Patienten, Harnleitertumore bei 8 Patienten und extraureterale Tumore bei 2 Patienten. Eine richtige Diagnose bei den Steinpatienten wurde durch IVU bei 49 von 72 Patienten und durch MRU bei 64 von 72 Patienten gestellt. In dieser Patientengruppe wurden durch die MRU zwei falsche Diagnosen gestellt. Fehlende Kontrastmittelausscheidung war der Hauptgrund für Versagen der IVU. 3 der 8 Patienten mit Harnleitertumoren wurden durch die IVU richtig dignostiziert, bei dreien wurde eine falsche Diagnose gestellt. Durch die MRU konnten in dieser Gruppe 7 von 8 Patienten korrekt diagnostiziert werden, es wurde keine falsche Diagnose erhoben. IVU wird zunächst die Standarduntersuchungstechnik zur Darstellung des oberen Harntraktes bleiben, aber durch diese Studie konnte die Möglichkeit gezeigt werden, die in der MRU in Kombination mit Gadolinium und Furosemid liegt. Die größte Bedeutung dieser Untersuchung liegt in der urographisch stummen Niere, bei Untersuchungen in der Schwangerschaft, bei Kindern und bei Patienten mit Kontrastmittelunverträglichkeit.

  7. Treibhausgasemissionen unter Bewässerung und unterschiedlicher Stickstoffdüngungsintensität auf einem Sandboden in Nord-Ost-Deutschland


    Trost, Benjamin


    Ziel der Arbeit ist die Gesamtbilanzierung der Treibhausgasemissionen eines Anbausystems unter Bewässerung auf einem Sandboden. Die dazu aufgestellte Treibhausgasbilanz basiert auf langjährigen Datenreihen zu Erträgen und Corg-Vorräten eines Dauerfeldversuchs sowie auf zweijährigen im Feldversuch durchgeführten N2O-Messungen. Die durchgeführten Untersuchungen zum Einfluss der Bewässerung auf die Corg-Vorräte zeigen, dass der Einsatz von mineralischem Stickstoffdünger und Bewässerung auf einem...

  8. Effervescent N-Acetylcysteine Tablets versus Oral Solution N-Acetylcysteine in Fasting Healthy Adults: An Open-Label, Randomized, Single-Dose, Crossover, Relative Bioavailability Study

    Directory of Open Access Journals (Sweden)

    Spencer C. Greene, MD, FACEP, FACMT


    Conclusions: Data from this study of a single dose of 11 g oral NAC demonstrated that effervescent NAC tablets and oral solution NAC met the regulatory criteria for bioequivalence in fasting healthy adult subjects. Effervescent NAC tablets appear to be a more palatable alternative for treatment of acetaminophen overdose. identifier: NCT02723669.

  9. Damage characterisation of silicon carbides for applications in gas turbines in complex load conditions; Charakterisierung des Schaedigungsverhaltens von Siliciumcarbiden fuer den Einsatz in Gasturbinen unter komplexen Beanspruchungsbedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Nestle, E.


    A tensile test facility for simultaneous thermal, mechanical and corrosive loading was developed and constructed for the purpose of characterizing the damage characteristics of ceramic high-temperature materials. Apart from tensile tests for up to 830 h, tests were also carried out on four-point bending test pieces and disk-shaped oxidation test pieces. The experiments were made at 1450 - 1550 C in dry or moist air. The materials investigated were one hot-pressed silicon carbide and two sintered silicon carbides. [German] Um keramische Hochtemperaturwerkstoffe bezueglich ihres Schaedigungsverhaltens charakterisieren zu koennen, wurde im Rahmen der vorliegenden Arbeit eine Zugpruefanlage zur gleichzeitigen Beanspruchung unter thermischen, mechanischen und korrosiven Bedingungen entwickelt und aufgebaut. Neben den in dieser Anlage durchgefuehrten Zugpruefungen mit Versuchszeiten bis zu 830 h wurden begleitende Untersuchungen an Vierpunkt-Biegeproben und scheibenfoermigen Oxidationsproben durchgefuehrt. Die Versuche konzentrierten sich auf den Temperaturbereich 1450-1550 unter trockenen und feuchten Luftatmosphaeren. Bei den untersuchten Werkstoffen handelte es sich um eine heissgepresste und zwei gesinterte Siliciumcarbid-Qualitaeten. (orig.)

  10. Investigations of coal ignition in a short-range flame burner using optical measuring systems; Untersuchungen zur Kohlezuendung am Flachflammenbrenner unter Verwendung optischer Messtechnik

    Energy Technology Data Exchange (ETDEWEB)

    Hackert, G.; Kremer, H.; Wirtz, S. [Bochum Univ. (Germany). Lehrstuhl fuer Energieanlagentechnik


    The short-range flame burner and the KOALA reactor of DMT are experimental facilities for realistic simulation of coal conversion processes at high temperatures and pressures in atmospheric conditions. The TOSCA system enable measurements of temperatures, sizes, shapes and velocities of the fuel particles, which serve as a basis for a three-dimensional simulation model of coal combustion. In the future, further parameter studies will deepen the present knowledge of coal dust combustion under pressure and enable optimisation of the numerical models for simulation of industrial-scale systems for coal dust combustion under pressure. [Deutsch] Mit dem Flachflammenbrenner und dem KOALA-Reaktor der DMT stehen Versuchsapparaturen zur Verfuegung, mit deren Hilfe die Kohleumwandlungsprozesse bei hohen Temperaturen unter Druck und unter atmosphaerischen Bedingungen realistisch wiedergegeben werden. Das TOSCA-System erlaubt dabei die Bestimmung von Temperaturen, Groessen, Formen und Geschwindigkeiten der Brennstoffpartikel. Diese Daten liefern die Grundlage fuer die Erstellung eines dreidimensionalen Simulationsmodells zur Modellierung der Kohleverbrennung. In Zukunft werden weitere Parameterstudien das Verstaendnis der Kohlenstaubdruckverbrennung vertiefen und ein Optimierung der numerischen Modelle ermoeglichen, so dass die Simulation grosstechnischer Kohlenstaubdruckverbrennungsanlagen realisiert werden kann. (orig.)

  11. Carbon dioxide digital subtraction angiography using a new gas management system; Digitale Subtraktionsangiographie mit Kohlendioxid unter Verwendung eines neuen Gasdosiersystems

    Energy Technology Data Exchange (ETDEWEB)

    Schmitz-Rode, T.; Alzen, G.; Guenther, R.W. [Klinik fuer Radiologische Diagnostik, Universitaetsklinikum der RWTH Aachen (Germany)


    Purpose: The clinical evaluation of digital subtraction angiography with carbon dioxide using a newly developed low-tech CO{sub 2}-dosage- and injection system. Method and patients: The hand-held system (CO{sub 2} angio set) consists of a dosage chamber in connection with a special stopcock to apportion the gas. By optimising injection volume and pressure steady gas flow characteristics are approximated. A safety design prevents unintentional gas injection volume and pressure steady gas flow characteristics are approximated. A safety design prevents unintentional gas injection. CO{sub 2} arteriographies were performed on 185 patients. Main indications were renal insufficiency and a history of adverse reactions to iodinated contrast media. In patients with femoral connula access, catheterless reflux angiography was performed. Results: The injection system provided complete and coherent visualisation of the abdominal aorta, visceral, pelvic, and lower limb arteries via catheter (71 cases) or via femoral cannula using reflux technique (114 cases). Stenoses, occlusions, and collaterals were assessable. Employing the gas reflux over the aortic bifurcation bilateral run-off studies up to the calf trifurcation were performed via unilateral femoral cannula. Use of a dedicated stacking software improved image quality of distal femoral, popliteal and calf arteries. Conclusion: The CO{sub 2} management system allows adequate imaging of the arteries below the diaphragm. Ease and safety of use and low costs are advantageous. (orig.) [Deutsch] Ziel: Die klinische Erprobung der digitalen Subtraktionsangiographie mit Kohlendioxid unter Verwendung eines neuentwickelten, einfachen Gasdosier- und Injektionssystems. Methode und Patienten: Eine Dosierkammer mit einstellbarem Volumen in Verbindung mit einem Spezial-Hahn (CO{sub 2}-Angio-Set) portioniert das Gas. Durch Optimierung von Injektionsvolumen und -druck wurde eine konstante Ausstroemcharakteristik angenaehert. Das System wurde

  12. Ein (neuer Blick auf die Konkurrenz unter Frauenforscherinnen A (New Look at Competition Among Women Scholars in Women’s Studies

    Directory of Open Access Journals (Sweden)

    Anina Mischau


    Full Text Available Die Solidarität unter Frauenforscherinnen wird bis heute als politisch-ideologisches Postulat der Frauenforschung aufrechterhalten, obwohl im Zuge einer zunehmenden Institutionalisierung und Professionalisierung bei einer gleichzeitig nahezu unveränderten Marginalisierung der Frauenforschung an deutschen Hochschulen eine stärker werdende Konkurrenz auch unter Frauenforscherinnen zu beobachten ist. Sünne Andresen geht der Frage nach den möglichen Ursachen von Konkurrenz unter Frauenforscherinnen in der Hochschule nach. Diese Fragestellung ist in der Frauen- und Geschlechterforschung nicht neu, wenngleich sie bislang eher allgemein auf die Konkurrenz unter Wissenschaftlerinnen bezogen worden ist. Innovativ ist jedoch, dass die Autorin hierfür den subjektwissenschaftlichen Ansatz der Kritischen Psychologie und die Habitus-Feld-Konzeption Bourdieus als theoretischen Bezugsrahmen auswählt und damit deutlich über die vorherrschenden subjektzentrierten oder strukturtheoretischen Erklärungsansätze hinausgeht. Die empirische Basis ihrer Studie sind sechs qualitative Interviews mit Habilitandinnen der geistes- und sozialwissenschaftlichen Fächer, die sich auf die Frauen- und Geschlechterforschung spezialisiert haben. Insgesamt arbeitet die Autorin interessante Aspekte möglicher Ursachen des Konkurrenzverhaltens unter und der Konkurrenzerfahrungen von Frauenforscherinnen heraus. Sie bleibt der Leserin aber mindestens genauso viele Antworten schuldig, wie sie durch die dargestellten „empirischen Ergebnisse" zu geben vermag.Until the present day, solidarity among feminist scholars is upheld as an ideological-political postulate in this particular scholarly community. These views continue to be upheld despite the increasing institutionalisation and professionalisation of research in women’s studies research. These institutional changes result in an increased sense of competition among feminist scholars, while women’s studies continue to

  13. Suplementação de N-acetilcisteína em pacientes infectados pelo HIV submetidos ao primeiro tratamento anti-retroviral: Avaliação do efeito sobre a carga viral, TNF-α, IL-6, IL-8, β2-microglobulina, IgA, IgG e IgM, haptoglobina e α1-glicoproteína ácida N-acetylcysteine supplementation of HIV-infected patients under the first anti-retroviral treatment: Evaluation of the effect on viral load, TNF-α, IL-6, IL-8, β2-microglobulin, IgA, IgG, IgM, haptoglobin and α1-acid glycoprotein

    Directory of Open Access Journals (Sweden)

    Aricio Treitinger


    alterations are characterized by elevated levels of tumor necrosis factor alpha (TNF-α, interleukin 8 (IL-8, β2-microglobulin, IgA, IgG, IgM, haptoglobin and a1-acid glycoprotein. The goal of this double blind placebo-controlled study was to evaluate the effect of N-acetylcysteine supplementation on virological, immunological and inflammatory markers in 24 HIVinfected individuals who were taking their first anti-retroviral therapy. Eleven individuals were treated with anti-retroviral therapy plus placebo supplementation and thirteen were treated with anti-retroviral therapy plus 600 mg/day of Nacetylcysteine. The levels of the studied markers were evaluated at the day before and after 60, 120 and 180 days of treatment. In both groups a significant decrease in serum levels of TNF-α (p=0.0001, IL-6 (p>0.05, IL-8 (p=0.0001, b2 microglobulin (p=0.0005, IgA (p=0.007, IgG (p=0.001, IgM (p=0.0001, haptoglobin (p=0.0001 e α1-acid glycoprotein (p=0.012 was found due to anti-retroviral therapy. N-acetylcysteine supplementation had no additive or synergistic effects on the studied parameters. In conclusion, N-acetylcysteine had no additional beneficial effects, at least at the dose used in this study, on the treatment of HIV-infected patients under anti-retroviral therapy.

  14. Islands sinking; Land unter

    Energy Technology Data Exchange (ETDEWEB)

    Zimmermann, Joerg-Rainer


    The urbanization is a global trend. Today, 22 of the 30megacities, each with more than 10 million inhabitants, are located in developing countries and emerging countries. These megacities consume the most electricity: About two-thirds of the global consumption of electricity and heat are consumed in megacities. At the same time, the prosperity is concentrated in monopolies: In the last 50 years, the per capita income of the urban population has increased by about 150 %. The productivity of urban areas increases. Thus, cities are of central importance to our culture. If global warming is not stopped, climate change hits them with full force.

  15. Ironieentwicklung unter interaktionslinguistischer Perspektive


    Kotthoff, Helga


    Der vorliegende Aufsatz gibt Einblick in ein Pilotprojekt zur Entwicklung von Ironie und vergleichbaren Formen der Kommunikation einer Bewertungskluft in Interaktionssituationen mit Schulkindern. Um Ironie zu verstehen, muss man davon ausgehen, dass Äußerungen verschiedene Bedeutungsschichten haben können. Man kann diesem Typus indirekten Sprechens nur Sinn zuordnen, wenn man generelle Haltungen des Sprechers inferieren kann, und diese Haltung dann gleichzeitig zum Ausgangspunkt nimmt, auch s...

  16. Visusminderung unter Silikon

    DEFF Research Database (Denmark)

    Dornonville de la Cour, Morten; Lux, Anja; Heegaard, Steffen


    Silicone oil is used as intravitreal tamponading agent in surgery for rhegmatogenous retinal detachment (RRD) cases complicated with proliferative vitreoretinopathy (PVR). Recently, a number of case series have appeared where profound central visual loss has been found in eyes after uncomplicated...... vitrectomy with silicone tamponade for RRD in eyes with seemingly good visual potential. Several reports have demonstrated the migration of silicone oil droplets into the retina and the optic nerve, others the widespread loss of myelinated optic nerve fibres. These reports are reviewed, and it is concluded...... that caution is warranted when silicone oil is used in eyes with good visual potential. Finally the additional danger of central visual loss should be taken into consideration when deciding to use silicone oil or gas as intravitreal tamponade....

  17. Crack initiation and crack growth in high temperature materials under cyclic thermal stresses; Rissinitiierung und Risswachstum in Hochtemperaturwerkstoffen unter zyklisch thermischer Beanspruchung

    Energy Technology Data Exchange (ETDEWEB)

    Gruen, C.


    The high temperatures of use in drive units, such as the combustion chamber or the hot gas turbine, for example, usually cause high temperature changes. Great temperature differences occur for short periods in the components, and thermal shock is produced. In this work, theoretical and experimental investigations are introduced on crack initiation and crack growth in high temperature materials under cyclic thermal stresses. The experiments were carried out with the inter-metallic phase Ni{sub 3}Al, the nickel-based alloy Nimonic 80A and the iron-based alloy PM 2000 strengthened by oxide dispersion (ODS). A characteristic crack appearance picture was found for each material, which was examined more closely. The stresses occurring in the sample during one cycle were calculated with the aid of the finite element program ABAQUS, knowing the specific material parameters. Based on the linear-elastic fracture mechanics, stress intensity factors were calculated on the superimposition principle. Using the material data from isothermal crack propagation experiments, the prediction of fatigue crack spread with cyclic thermal stresses is compared with the experimental findings. (orig./AKF) [Deutsch] Die hohen Einsatztemperaturen in Antriebsaggregaten wie z.B. der Brennkammer oder der Heissgasturbine bedingen in der Regel hohe Temperaturwechsel. Dabei treten kurzzeitig grosse Temperaturunterschiede in den Bauteilen auf, ein Thermoschock wird erzeugt. In der vorliegenden Arbeit werden theoretische und experimentelle Untersuchungen zur Rissinitiierung und zum Risswachstum in Hochtemperaturwerkstoffen unter zyklisch thermischer Belastung vorgestellt. Die Experimente wurden mit der intermetallischen Phase Ni{sub 3}Al, der Nickelbasislegierung Nimonic 80A und der oxid-dispersionsverfestigten (ODS) Eisenbasislegierung PM2000 durchgefuehrt. Fuer jeden Werkstoff stellte sich ein charakteristisches Risserscheinungsbild dar, das naeher untersucht wurde. Die in der Probe auftretenden

  18. Dynamic oxygen transfer measurements under operating conditions as a basis for the optimization of ventilation systems; Dynamische Sauerstoffeintragsmessungen unter Betriebsbedingungen als Grundlage zur Optimierung von Belueftungssystemen

    Energy Technology Data Exchange (ETDEWEB)

    Libra, J.A.; Biskup, M.; Wiesmann, U. [Technische Univ. Berlin (Germany). Inst. fuer Verfahrenstechnik; Sahlmann, C.; Gnirss, R. [Berliner Wasserbetriebe, Berlin (Germany)


    The largest single energy consumer at sewage treatment plants is the ventilation system of activated sludge tanks. This is why controlling and optimizing ventilation systems is the most appropriate approach to the cutting down of energy costs. The present paper reports on measurements of dynamic oxygen transfer by means of the off-gas method under operating conditions at the Berlin-Ruhleben sewage treatment plant. (orig.) [German] Der groesste Einzelenergieverbraucher auf Klaerwerken ist das Belueftungssystem von Belebungsbecken. Deshalb ist die Kontrolle und Optimierung der Belueftungssysteme der geeignete Weg zur Verringerung der Energiekosten. In diesem Beitrag wird ueber Messungen des dynamischen Sauerstoffeintrags mit der Abluft-Methode unter Betriebsbedingungen im Klaerwerk Berlin-Ruhleben berichtet. (orig.)

  19. Harmonisation of fire testing of conveyor belts for underground applications within the EC; Harmonisierung der brandtechnischen Pruefverfahren von Foerdergurten fuer den Einsatz unter Tage innerhalb der EG

    Energy Technology Data Exchange (ETDEWEB)

    Foit, W. [DMT-Gesellschaft fuer Forschung und Pruefung mbH, Dortmund (Germany). Fachstelle fuer Brand- und Explosionsschutz unter Tage - Versuchsgrube Tremonia


    The mining countries of the EC still have different regulations for fire testing of conveyor belts. The test methods will be harmonized for the Common Market, and requirements on conveyor belts will be standardized within the EC. A new EC standard will be drawn up. [Deutsch] Zur Zeit sind in den bergbaubetreibenden Laendern der EG noch unterschiedliche Verfahren zur brandtechnischen Pruefung von Foerdergurten vorgeschrieben. Mit dem Inkrafttreten des Gemeinsamen Marktes und der damit zusammenhaengenden Forderung nach Abbau von Handelshemmnissen sollen diese Pruefverfahren harmonisiert werden. Ziel ist es, die an die Foerdergurte zu stellenden brandtechnischen Anforderungen innerhalb der EG zu vereinheitlichen. Es soll deshalb eine europaeische Norm zur Beurteilung des Brandverhaltens und der Brandausbreitung an Foerdergurten fuer den Kohlenbergbau unter Tage erstellt werden. (orig./MSK)

  20. [Dimitri Steinke: Die Zivilrechtsordnungen des Baltikums unter dem Einfluss ausländischer, insbesondere deutscher Rechtsquellen. (Osnabrücker Schriften zur Rechtsgeschichte, Bd. 16.) V&R unipress. Göttingen 2009. 243 S. ISBN 978-3-89971-573-6] / M

    Index Scriptorium Estoniae

    Luts-Sootak, Marju, 1966-


    Arvustus: Dimitri Steinke. Die Zivilrechtsordnungen des Baltikums unter dem Einfluss ausländischer, insbesondere deutscher Rechtsquellen. (Osnabrücker Schriften zur Rechtsgeschichte ; 16). Göttingen, 2009

  1. N-acetylcysteine improves arterial vascular reactivity in patients with chronic kidney disease

    DEFF Research Database (Denmark)

    Wittstock, Antje; Burkert, Magdalena; Zidek, Walter


    Patients with stage 5 chronic kidney disease show increased cardiovascular morbidity and mortality that are partly related to impaired arterial vascular reactivity. We investigated whether intravenous administration of the antioxidant acetylcysteine improves arterial vascular reactivity in these ...

  2. Protective Effect of N-Acetylcystein and Resveratrol on Ischemia-Reperfusion Injury in Rat Ovary

    Directory of Open Access Journals (Sweden)

    Avni Kılıç


    Full Text Available Objective: The aim of this study is evaluating the protective activity of N-acetyl cysteine and resveratrol treatment against ischemia - reperfusion damage created experimentally in rat ovaries. Methods: 42 female Wistar rats were used in our study. Rats were separated randomly into six groups consisting of seven rats as sham, torsion, torsion- detorsion, torsion-detorsion+saline, torsion-detorsion+resveretrol (20 mg/kg and torsion- detorsion+N-acetyl cysteine (150 mg/kg. Except Sham, ovarian torsion procedure was implemented to all other groups for 2 hours. Detorsion procedure was implemented to other groups for 2 hours, except the torsion group. Medications were given through intraperitoneal way half an hour before the detorsion procedure in saline (two milliliter, resveratrol (20 mg/kg and N-acetyl cysteine (150 mg/kg groups. Then, 2 ml of blood samples were drawn for markers of oxidative stress and tumour necrosis factor-alpha (TNF-α work and the ovaries, which were torsioned for the histologic examination, were ex­tracted from all rats. Edema, congestion, hemorrhage, leuko­cyte infiltration and degeneration of follicles were evaluated by histopathological examination. Results: According to histopathologic damage scores, the least damage was seen in sham group and the most damage was seen T-DT group (1.00±0.81 vs. 11.00±1.15, respectively; p<0.001. It was seen that resveratrol and N-acetyl cysteine treatments were effective in decreasing tissue damage (total damage score average 83.85±0.89 vs. 3.85±0.89, respec­tively; p<0.001, and on the other hand there was not any dif­ference between resveratrol and N-acetyl cysteine treatments (p=0.966. Besides, it was determined that oxidative stress levels were higher in torsion - detorsion group and the resve­ratrol and N-acetyl cysteine treatment caused a significant de­crease in oxidative stress levels. In additionally, the reductions of TNF-α levels were found to be equally effective in both drugs (8.68±1.88 vs. 7.85±2.08, P=0.968. Conclusion: Presented study showed that resveratrol and N-acetyl cysteine treatment can be effective in preventing tissue damage and oxidative stress, which is induced by ischemia-reperfusion that is created in rat ovaries. On the other hand, no difference was found between the resveratrol and N-acetyl cysteine with regards to protective activity.

  3. No net splanchnic release of glutathione in man during N-acetylcysteine infusion

    DEFF Research Database (Denmark)

    Poulsen, H E; Vilstrup, H; Almdal, T


    Glutathione and amino acid concentrations were measured in arterial and hepatic vein plasma in four healthy volunteers and two patients with cirrhosis. There was no significant splanchnic efflux of glutathione (95% confidence limits, -0.501 to 0.405 mumol/min). After infusion of N...... to 0.97 +/- 0.11 (mean +/- SEM; p amino acids corresponded to an increased load on hepatic metabolic N conversion and transamination among nonessential amino acids. Splanchnic uptake of serine, alanine, cystine, isoleucine, and phenylalanine increased...... after NAC compatible with stimulated hepatic glutathione synthesis. In contrast to the rat, plasma glutathione in man probably originates mainly from extrahepatic tissues....

  4. Activity of MnSOD in chronically hypoxic rat heart: effect of N-acetylcysteine

    Czech Academy of Sciences Publication Activity Database

    Balková, P.; Neckář, Jan; Nováková, O.; Milerová, Marie; Kolář, František; Novák, F.


    Roč. 40, č. S1 (2006), S123-S123 ISSN 1071-5762. [Biennial Congress of the SFRRI /13./. 15.08.2006-19.08.2006, Davos] R&D Projects: GA MŠk(CZ) 1M0510 Institutional research plan: CEZ:AV0Z50110509 Keywords : chronic hypoxia * oxidative stress * heart Subject RIV: ED - Physiology

  5. Minocycline and N-acetylcysteine: A Synergistic Drug Combination to Treat Traumatic Brain Injury (United States)


    Furchgott Center for Neural and Behavioral Science, 450 Clarkson Avenue, Brooklyn, NY 11203, USA b State University of New York-Downstate Medical Center...later all groups were tested on open field and = 0.59, p N 0.5). On passive place avoidance, therewas no effect of treatment on distance lace avoidance

  6. Endothelial dysfunction in metabolic diseases: role of oxidation and possible therapeutic employment of N-acetylcysteine. (United States)

    Masha, A; Martina, V


    Several metabolic diseases present a high cardiovascular mortality due to endothelial dysfunction consequences. In the last years of the past century, it has come to light that the endothelial cells, previously considered as inert in what regards an eventual secretion activity, play a pivotal role in regulating different aspects of the vascular function (endothelial function). It was clearly demonstrated that the endothelium acts as a real active organ, owning endocrine, paracrine and autocrine modulation activities by means of which it is able to regulate the vascular homeostasis. The present review will investigate the relationship between some metabolic diseases and the endothelial dysfunction and in particular the mechanisms underlying the effects of metabolic pathologies on the endothelium. Furthermore, it will consider the possible therapeutic employment of the N-acetilcysteine in such conditions.

  7. Vitamin E and N-Acetylcysteine as Antioxidant Adjuvant Therapy in Children with Acute Lymphoblastic Leukemia

    Directory of Open Access Journals (Sweden)

    Youssef Al-Tonbary


    Full Text Available Although cancer therapies have experienced great success nowadays, yet the associated toxic response and free radicals formation have resulted in significant number of treatment-induced deaths rather than disease-induced fatalities. Complications of chemotherapy have forced physicians to study antioxidant use as adjunctive treatment in cancer. This study aimed to evaluate the antioxidant role of vitamin E and N-acetyl cysteine (NAC in overcoming treatment-induced toxicity in acute lymphoblastic leukaemia (ALL during the intensive period of chemo-/radiotherapy, almost the first two months of treatment. Forty children newly diagnosed with ALL were enrolled in this study. Twenty children (group I have taken vitamin E and NAC supplementations with chemotherapy and the other twenty children (group II have not taken any adjuvant antioxidant therapy. They were evaluated clinically for the occurrence of complications and by the laboratory parameters (blood levels of glutathione peroxidase (Glu.PX antioxidant enzyme, malondialdehyde (MDA, tumor necrosis factor- (TNF-, liver enzymes, and bone marrow picture. Results revealed reduced chemotherapy and radiotherapy toxicity as evidenced by decreasing level of MDA, increasing level of Glu.Px and decreased occurrence of toxic hepatitis, haematological complications, and need for blood and platelet transfusions in group I compared to group II. We can conclude that vitamin E and NAC have been shown to be effective as antioxidant adjuvant therapy in children with ALL to reduce chemo-/radiotherapy-related toxicities during the initial period of treatment.

  8. Is N-acetylcysteine effective in the treatment of pulmonary fibrosis?

    Directory of Open Access Journals (Sweden)

    Alejandro Jeldres Pulgar


    Full Text Available Resumen La fibrosis pulmonar idiopática es una enfermedad respiratoria crónica y progresiva, que en etapas finales conlleva una alta mortalidad. Se han planteado múltiples opciones terapéuticas, entre ellas la N-acetilcisteína, pero su rol no está claramente establecido. Utilizando la base de datos Epistemonikos, la cual es mantenida mediante búsquedas en 30 bases de datos, identificamos ocho revisiones sistemáticas que en conjunto incluyen 16 estudios aleatorizados. Realizamos un metanálisis y tablas de resumen de los resultados utilizando el método GRADE. Concluimos que N-acetilcisteína probablemente aumenta el riesgo de hospitalización y exacerbaciones. Si bien no está claro si esto conlleva un aumento de mortalidad porque la certeza de la evidencia es muy baja, en general existe consenso en que no debiera utilizarse, a no ser que sea en el contexto de un nuevo estudio clínico.

  9. Arsenic induces apoptosis in mouse liver is mitochondria dependent and is abrogated by N-acetylcysteine

    International Nuclear Information System (INIS)

    Santra, Amal; Chowdhury, Abhijit; Ghatak, Subhadip; Biswas, Ayan; Dhali, Gopal Krishna


    Arsenicosis, caused by arsenic contamination of drinking water supplies, is a major public health problem in India and Bangladesh. Chronic liver disease, often with portal hypertension occurs in chronic arsenicosis, contributes to the morbidity and mortality. The early cellular events that initiate liver cell injury due to arsenicosis have not been studied. Our aim was to identify the possible mechanisms related to arsenic-induced liver injury in mice. Liver injury was induced in mice by arsenic treatment. The liver was used for mitochondrial oxidative stress, mitochondrial permeability transition (MPT). Evidence of apoptosis was sought by TUNEL test, caspase assay and histology. Pretreatment with N-acetyl-L-cysteine (NAC) was done to modulate hepatic GSH level. Arsenic treatment in mice caused liver injury associated with increased oxidative stress in liver mitochondria and alteration of MPT. Altered MPT facilitated cytochrome c release in the cytosol, activation of caspase 9 and caspase 3 activities and apoptotic cell death. Pretreatment of NAC to arsenic-treated mice abrogated all these alteration suggesting a glutathione (GSH)-dependent mechanism. Oxidative stress in mitochondria and inappropriate MPT are important in the pathogenesis of arsenic induced apoptotic liver cell injury. The phenomenon is GSH dependent and supplementation of NAC might have beneficial effects

  10. Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in Rats

    Directory of Open Access Journals (Sweden)

    Hasan Kahraman


    Full Text Available Objective: Methotrexate (MTX is known to have deleterious side effects on lung tissue. We aimed to investigate the effects of erythropoietin (EPO and N-acetyl-cysteine (NAC on MTX-induced lung injury in rats. Study Design: Animal experiment. Material and Methods: Twenty-six female Sprague-Dawley rats were divided into 4 groups. Sham group, 0.3 mL saline; MTX group, 5 mg/kg MTX; EPO group, 5mg/kg MTX and 2000 IU/kg EPO; NAC group, 5 mg/kg MTX and 200 mg/kg NAC were administered once daily for 4 consecutive days. Malondialdehyde (MDA, superoxide dismutase (SOD, catalase (CAT and inflammation and congestion scores in lung tissues were evaluated. Results: In MTX group MDA were significantly higher, CAT and SOD were significantly lower than in sham, EPO and NAC groups (p0.005. In group MTX both scores were significantly higher than in sham (p<0.005. The congestion score of group MTX was significantly higher than those of group EPO and NAC (p<0.005. Conclusion: EPO and NAC have significant preventive effects on MTX-induced lung injury in rats. Decreased antioxidant capacity and increased MDA level may cause the oxidative damage in MTX group. Also, higher antioxidant capacity and lower MDA level may be a response to oxidative stress in EPO and NAC groups.

  11. Administration of N-acetylcysteine causes beneficial posttranslational modifications of transthyretin in hemodialysis patients

    DEFF Research Database (Denmark)

    Scholze, Alexandra


    of the protein transthyretin (TTR) in plasma from hemodialysis patients in a randomized, placebo-controlled study in vivo and after administration to plasma in vitro. TTR was selected due to its low molecular weight and the free cysteine residue in the polypeptide chain, which is known to be extensively modified...... by formation of mixed disulfides. METHODS: Plasma levels of TTR were determined by a non-commercial enzyme-linked immunosorbent assay (ELISA) using polyclonal rabbit anti-human TTR antibodies. Spectra of immunoprecipitated TTR were obtained using an AutoflexSpeed matrix assisted laser desorption ionization-time......Analysis. TTR variants were expressed as relative amounts of the summed intensity of all observed TTR variants. RESULTS: The administration of NAC during a hemodialysis session resulted in a substantial increase of native TTR from median 15% (range 8.8-30%) to median 40% (37-50) and a reduction of S...

  12. Add-on treatment with N-acetylcysteine for bipolar depression

    DEFF Research Database (Denmark)

    Ellegaard, Pernille Kempel; Licht, Rasmus Wentzer; Poulsen, Henrik Enghusen


    BACKGROUND: Oxidative stress and inflammation may be involved in the development and progression of mood disorders, including bipolar disorder. Currently, there is a scarcity of useful treatment options for bipolar depressive episodes, especially compared with the efficacy of treatment for acute ...

  13. N-acetylcysteine ameliorates contrast‑induced kidney injury in rats with unilateral hydronephrosis. (United States)

    Xia, Qiang; Liu, Chunxiao; Zheng, Xia


    The aim of the present study was to investigate the protective effects of N‑acetylcysteine (NAC) on contrast‑induced acute kidney injury in rats with unilateral hyronephrosis. Eighty‑two male Sprague Dawley rats were randomized to undergo sham operation (n=14) or unilateral ureteral obstruction (UUO) (n=68). After 3 weeks, the UUO animals were randomized to three groups: NAC gastric perfusion, UUO+iohexol+NAC (n=24); normal saline perfusion, UUO+iohexol (n=24); and controls, UUO (n=20). After 3 days, UUO+iohexol+NAC and UUO+iohexol rats were injected with iohexol. One day after contrast, half of the rats were sacrificed to assess the pathological changes to the kidneys, serum creatinine, serum neutrophil gelatinase‑associated lipocalin (NGAL), renal cell apoptosis rate and expression of apoptosis regulators Bcl‑2/Bax. The remaining rats underwent obstruction relief and were analyzed 3 weeks later. Compared with the controls, serum NGAL levels were high in UUO+iohexol rats 1 day following injection and 3 weeks after obstruction relief, but UUO+iohexol+NAC rats exhibited lower serum NGAL levels compared with UUO+iohexol rats (all Pmodeling, UUO+iohexol rats exhibited a significantly higher apoptosis rate of renal tubular cells, higher expression of Bax mRNA, and lower ratio of Bcl‑2/Bax (all Prelief, UUO+iohexol+NAC rats exhibited a lower apoptosis rate, lower Bax mRNA expression, higher expression of Bcl‑2 mRNA and higher ratio of Bcl‑2/Bax (all P<0.05) compared with day 1 following drug administration. The prophylactic use of NAC reduced the apoptotic rate of renal tubular cells following contrast exposition, which was accompanied by changes in the expression of Bcl‑2/Bax mRNA.

  14. The effect of N-acetylcysteine and melatonin in adult spontaneously hypertensive rats with established hypertension

    Czech Academy of Sciences Publication Activity Database

    Pecháňová, Olga; Zicha, Josef; Paulis, Ĺudovít; Zenebe, W.; Dobešová, Zdenka; Kojšová, S.; Jendeková, L.; Sládková, M.; Dovinová, I.; Šimko, F.; Kuneš, Jaroslav


    Roč. 561, 1-3 (2007), s. 129-136 ISSN 0014-2999 R&D Projects: GA MZd(CZ) NR7786; GA ČR GA305/03/0769 Grant - others:VEGA(SK) 2/6148/26; VEGA(SK) 1/3429/06; VEGA(SK) 1/3442/26; APVT(SK) 51-027404; APVT(SK) 51-017902 Institutional research plan: CEZ:AV0Z5011922 Keywords : NO synthase * oxidative load * genetic hypertension Subject RIV: ED - Physiology Impact factor: 2.376, year: 2007

  15. Vitamin E and N-Acetylcysteine as Antioxidant Adjuvant Therapy in Children with Acute Lymphoblastic Leukemia (United States)

    Al-Tonbary, Youssef; Al-Haggar, Mohammad; EL-Ashry, Rasha; EL-Dakroory, Sahar; Azzam, Hanan; Fouda, Ashraf


    Although cancer therapies have experienced great success nowadays, yet the associated toxic response and free radicals formation have resulted in significant number of treatment-induced deaths rather than disease-induced fatalities. Complications of chemotherapy have forced physicians to study antioxidant use as adjunctive treatment in cancer. This study aimed to evaluate the antioxidant role of vitamin E and N-acetyl cysteine (NAC) in overcoming treatment-induced toxicity in acute lymphoblastic leukaemia (ALL) during the intensive period of chemo-/radiotherapy, almost the first two months of treatment. Forty children newly diagnosed with ALL were enrolled in this study. Twenty children (group I) have taken vitamin E and NAC supplementations with chemotherapy and the other twenty children (group II) have not taken any adjuvant antioxidant therapy. They were evaluated clinically for the occurrence of complications and by the laboratory parameters (blood levels of glutathione peroxidase (Glu.PX) antioxidant enzyme, malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), liver enzymes, and bone marrow picture). Results revealed reduced chemotherapy and radiotherapy toxicity as evidenced by decreasing level of MDA, increasing level of Glu.Px and decreased occurrence of toxic hepatitis, haematological complications, and need for blood and platelet transfusions in group I compared to group II. We can conclude that vitamin E and NAC have been shown to be effective as antioxidant adjuvant therapy in children with ALL to reduce chemo-/radiotherapy-related toxicities during the initial period of treatment. PMID:19960046

  16. N-acetylcysteine manipulation fails to elicit an increase in glutathione in a teleost model

    DEFF Research Database (Denmark)

    Birnie-Gauvin, Kim; Larsen, Martin Hage; Aarestrup, Kim


    and vegetable shortening, at two different concentrations (100 and 400 mg/kg), with the appropriate controls and shams, under controlled laboratory settings. We found that NAC failed to elicit an increase in GSH over three time periods and concluded that NAC is not an effective method to enhance GSH levels...

  17. Hydrogen diffusion into fatigue cracks of aluminium alloy 6013 in a corrosive environment; Wasserstoffeinlagerung an Ermuedungsrissen der Aluminiumlegierung 6013 unter korrosiver Umgebung

    Energy Technology Data Exchange (ETDEWEB)

    Lenk, Christian Alexander


    The author attempted a time-resolved detection of raised hydrogen concentrations in the plastic deformation region of fatigue cracks in an aluminium test piece deformed by cyclic stress in a corrosive environment. Mechanical material parameters like the crack propagation velocity under cyclic stress change dramatically in a corrosive environment. This is assumed to be caused by hydrogen diffusion, but so far there is no method that reliably measures additional hydrogen from the corrosive environment. For this reason, a special analytical configuration was set up which makes use of the thermal desorption method. First, chips with a thickness of about 20 micrometers are sawed out of the test specimen in high-vacuum conditions. The chips fall into a hot melting vessel in a UHV chamber, where the hydrogen contained in the chips is released. The resulting pressure increase is recorded by a mass spectrometer. A hydrogen profile of the test specimen is obtained by assigning the chip position to the signal. For the corrosive medium in which the test specimen is immersed during crack initiation, i.e. NaCl solution, heavy water was used. This makes it possible to distinguish between the hydrogen contained in a piece of technical aluminium alloy (AA6013) and the deuterium diffusing in from the corrosive fluid. The deuterium is found exclusively in the test piece volume in the strongly plastically deformed region surrounding the fatigue crack. (orig.) [German] Das Ziel der vorliegenden Arbeit besteht im ortsaufgeloesten Nachweis einer erhoehten Wasserstoffkonzentration im plastisch deformierten Bereich von Ermuedungsrissen einer unter korrosiver Umgebung zyklisch verformten Aluminiumprobe. Mechanische Materialparameter wie z.B. die Rissausbreitungsgeschwindigkeit unter zyklischer Belastung aendern sich drastisch in korrosiver Umgebung. Als Ursache fuer dieses Verhalten wird eine Eindiffusion von Wasserstoff vermutet, jedoch gibt es bisher keine Messung die den zusaetzlichen

  18. Predicting the service life of connecting rod and main bearings, taking into account different engine loads. Final report; Lebensdauervorhersage fuer Pleuel- und Hauptlager unter Beruecksichtigung unterschiedlicher Motorlastkollektive. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Berkowitz, B.; Lueneburg, B.; Liu, J.


    Connecting rod and main bearings of internal combustion engines are among the most heavily stressed components in modern engines. It was found that these engine components are increasingly reaching the limits of their strength and therefore become the components limiting engine life. However, there is no known process which makes a calculated prediction of the service life of modern connecting rod and main bearings possible, taking into account various engine loads. In this research project, a new concept for the calculated prediction of the service life of non-static highly loaded radial sliding bearings is worked out. The basis is a process of calculation which realistically determines the pressure and gap course in highly loaded sliding bearings. Apart from the viscosity depending on pressure and temperature, the elastic deformations of the system sliding bearing/conn rod are also taken into account. Calculations are done for different operating points for a complete cycle of a four stroke Diesel engine (Daimler-Benz OM 364 LA). The results are compared with the present conventional process of calculation and the effects which occur are discussed. Based on the elasto-hydrodynamic calculation, a method of calculating the service life of non-statically loaded sliding bearings, taking into account various motor loads, is worked out. (orig./HW) [Deutsch] Pleuel- und Hauptlager von Verbrennungskraftmaschinen zaehlen mit zu den spezifisch am hoechsten beanspruchten Bauteilen in modernen Motoren. Es hat sich gezeigt, dass vor allem diese Maschinenelemente zunehmend an die Grenzen ihrer Belastbarkeit stossen und so zum lebensdauerbegrenzenden Bauteil werden. Es ist jedoch kein Verfahren bekannt, das eine rechnerische Lebensdauervorhersage moderner Pleuel- und Hauptlager unter Beruecksichtigung unterschiedlicher Motorlastkollektive ermoeglicht. Im vorliegenden Forschungsvorhaben wird daher ein neuartiges Konzept zur rechnerischen Lebensdauervorhersage von instationaeren

  19. Presentation of the Well Tractor Concept and practical experience in extreme conditions; Vorstellung des Well Tractor Konzepts mit Erfahrungen bei Einsaetzen unter Extrembedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Kater, H.; Preiss, F. [Preussag Wireline- und Messservice, Edemissen (Germany)


    So-called extended reach boreholes with horizontal sections of more than 2000 m, short radius drilling, multilaterals and other variations are common enough. Problems occur when this type of borehole needs to be surveyed or modified. The contribution describes the newly developed ``Well Tracotor{sup circledR}`` technology and outlines its potential. (orig.) [Deutsch] Sogenannte Extended Reach Bohrungen mit Horizontalsektionen von mehr als 2.000 Meter Laenge, Short Radius Drilling, Multilaterals und andere Variationen sind bohrtechnisch einwandfrei durchfuehrbar und gehoeren zum gaengigen Repertoire der Bohrfirmen. Grosse Probleme entstehen jedoch wenn dieser Bohrungstyp vermessen, komplettiert oder aufgewaeltigt werden soll. Insbesondere Bohrlochmessfirmen haben es in den letzten ca. 20 Jahren versaeumt Innovationen zwecks oekonomischen Einsatzes ihrer Technologie in diesen Bohrungen bereitzustellen. Die Durchfuehrung von Perforationen und Bohrlochmessungen, das Setzen von Stopfen, Schneiden von Rohren und auch einfachste Slickline-Operationen sind bei einer Bohrlochneigung beginnend bei ca. 60 bis 80 nur unter Anwendung von kosten- und zeitintensiven Verfahren moeglich. Eine guenstige Alternative zu diesen Verfahren bietet der Well Tractor. Diese Technologie ermoeglicht das Einfahren von Geraeten, die ueblicherweise am Kabel oder Draht eingesetzt werden, in Horizontalbohrungen. Im Folgenden werden das technische Konzept, ein Vergleich mit alternativen Verfahren, und verschiedene Referenzen des Well Tracotors {sup trademark} dargestellt. Weiterhin wird auf die Modifikationen eingegangen, die noetig waren, um dieses Geraet den Gegebenheiten in tiefen, heissen Gasbohrungen anzupassen. Abschliessend soll durch einen Ausblick auf zum Teil schon im Feldtest befindliche Weiterentwicklungen das weitere Potential dieser Technologie aufgezeichnet werden. (orig.)

  20. MR-imaging of finger osteoarthritis: Morphology and cartrilage signal intensity before and after treatment with ademetionin; Magnetresonanztomographie der Fingerpolyarthrose: Morphologie und Knorpelsignalverhalten unter Ademetonintherapie

    Energy Technology Data Exchange (ETDEWEB)

    Koenig, H. [Radiologische Klinik, Abt. fuer Roentgendiagnostik und Nuklearmedizin, Univ.-Klinikum Steglitz, FU Berlin (Germany); Stahl, H. [Radiologische Klinik, Univ.-Klinikum Rudolf-Virchow, FU Berlin (Germany); Sieper, J. [Medizinische Klinik und Poliklinik, Univ.-Klinikum Steglitz, FU Berlin (Germany); Wolf, K.J. [Radiologische Klinik, Abt. fuer Roentgendiagnostik und Nuklearmedizin, Univ.-Klinikum Steglitz, FU Berlin (Germany)


    This report deals with a prospective study of 21 patients with finger osteoarthritis treated over a period of three months with either Ademetionin (therapy group: 14/21) or without (control group: 7/21). MR-Imaging was carried out before and after treatment using spin-echo and 3D-Flash sequences. Morphological parameters and signal intensity changes of the hyaline cartilage were evaluated. The increase of the cartilage signal intensity was significant in the therapy group, this can be interpreted as an structural improvement. Also a decrease of the cartilage signal intensity with age was found. The morphological parameters showed no significant changes in the therapy and control groups. (orig.) [Deutsch] Im Rahmen einer prospektiven Studie wurden magnetresonanztomographische Untersuchungen bei Patientinnen mit Fingerpolyarthrose vor und nach Ademetionintherapie durchgefuehrt. Es erfolgten zwei Messungen an einer Kontrollgruppe (n=7, keine Ademetionintherapie) und an der Therapiegruppe (n=14) im Abstand von drei Monaten. Zur Anwendung kamen eine Doppel-Spin-Echo-Sequenz sowie eine 3D-Flash-Sequenz. Ausgewertet wurden neben morphologischen Parametern auch der Signalintensitaetsverlauf im Bereich des hyalinen Gelenkknorpels der Interphalangealgelenke. Signifikant war der Knorpelsignalzuwachs unter Ademetionintherapie, dies kann als Zeichen einer strukturellen Befundbesserung interpretiert werden. Ebenfalls signifikant zeigte sich die Abnahme der Knorpelsignalintensitaet mit zunehmendem Lebensalter der Patientinnen. Bei den morphologischen Befundaenderungen konnte kein signifikanter Therapieeffekt festgestellt werden. (orig.)

  1. Self-management by firm, non-elastic adjustable compression wrap device [Translation of Druckmessungen unter Klettverschluss-Kompression - Selbstbehandlung durch feste, unelastische Beinwickelung

    Directory of Open Access Journals (Sweden)

    Giovanni Mosti


    Full Text Available Severe forms of chronic venous insufficiency and lymphedema require strong compression-pressure, which exceeds the pressure exerted by medical compression stockings (>40 mmHg. The aim was to investigate if patients are able to apply a Velcro-band compression device (Circaid Juxta Lite™ themselves with sufficient pressure. Thirty-one patients (CEAP C6=23, C5=5, C3=2, mixed ulcer=1 applied Juxta Lite™ on their own legs after a short instruction and were asked to readjust the pressure by their subjective feeling. Sub- bandage pressure was measured after application and 24 h later. In 30 patients without arterial occlusive disease the median sub- bandage pressure values on day 1 and day 2 were 44,5 mmHg (IQR 42-48, and 46 mmHg (IQR 44-48,25 respectively. One patient with an arterialvenous leg ulcer showed pressures of 34 and 36 mmHg. All measured pressure values corresponded to the pursued target range, demonstrating that adequate self application of Velcro bands is feasible and that patents can maintain this pressure by re-adjustment. Source: this paper is an abridged translation of Mosti G, Partsch H. Druckmessungen unter Klettverschluss-Kompression - Selbstbehandlung durch feste, unelastische Beinwickelung. Vasomed 2017;5:212-6.

  2. Deformation behavior and load limits of asphaltic concrete under the conditions of cores in embankment dams; Deformationsverhalten und Belastungsgrenzen des Asphaltbetons unter den Bedingungen von Staudammkerndichtungen

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, U.


    Based on the analysis of existing dams with asphaltic diaphragm and investigations in the three-phase-system of asphaltic concrete a recipe for the composition of asphaltic cores is recommended. For the construction, rest and operating period of an embankment dam the load and the reaction of the asphaltic concrete cores as well as the appearing stress and deformations are described. Extensive material testings have been performed and at 41 asphaltic concrete specimens triaxial stress controlled pressure and creeping tests have been carried out. The evaluation of the triaxial tests led to proportions of the main stress and deformation limits as criteria of breaking. Under application of the standard equation for nonlinear viscoelastic element-laws a rheonom element formulation was developed from the experiment data and transformed into its differential form. With this approach the stress and deformation behavior of watertight asphaltic diaphragm can be precalculated for a period up to 10 years. The applicability of this approach, which can be also used within FE-calculations as well, is illustrated in four examples. (orig.) [Deutsch] Nach der Analyse bestehender Staudaemme mit Asphaltbetonkerndichtung und Untersuchungen zum Dreiphasensystem Asphaltbeton wird eine Rezepturempfehlung fuer den Asphaltkerndichtungsbau aufgestellt. Fuer die Bau-, Ruhe- und Betriebsphase eines Staudammes werden die Beanspruchungen und Reaktionen der Asphaltbetonkerndichtung sowie die auftretenden Spannungen und Verformungen beschrieben. Nach umfangreichen Materialpruefungen sind an 41 Asphaltbetonpruefkoerpern triaxiale spannungsgesteuerte Druck-Kriechversuche durchgefuehrt worden. Die Auswertung der Triaxialversuche ergab ein Grenzhauptspannungsverhaeltnis und Deformationsgrenzen als Bruchkriterien. Unter Verwendung der Standarformulierung fuer nichtlineare viskoelastische Stoffgesetze wurde aus den Versuchsdaten ein rheonomer Stoffansatz entwickelt und in seine differentielle Form

  3. Municipal climate protection as a measure for sustainable energy supply under the conditions of globalization and liberalization? An empirical investigation considering the municipal actors and public utility companies; Kommunaler Klimaschutz als Instrument einer nachhaltigen Energieversorgung unter den Bedingungen von Globalisierung und Liberalisierung? Eine empirische Untersuchung unter besonderer Beruecksichtigung der Akteure Kommune und Stadtwerke

    Energy Technology Data Exchange (ETDEWEB)

    Bielitza-Mimjaehner, Ralf


    With the background of the threatening global warming that requires a reduction of greenhouse gas emission by 20 % until 2010 und by 80% until 2050 in Germany, t is reasonable to consider local or municipal climate protection activities. A climate protection politics ''from the bottom'' is not only contributing to a real greenhouse gas reduction, but also triggering the stagnant international climate politics and enhances the sustainable development on a local level. Due to this fact amongst others the Enquete commission of the German Bundestag ''sustainable energy supply under the conditions of globalization and liberalization'' identifies the municipal climate protection activities as important part within the mix of measures that will allow the initiation of an alteration of the actual energy system toward a sustainable energy supply in the long-run. The consequences of globalization and liberalization on the municipal level have not yet been discussed or considered by the Enquete commission. This thesis analyses the conditions induced by the globalization and liberalization on the municipal climate protection activities as constituent of a sustainable energy supply. The project is focusing on the municipal actors and the public utility companies as central point of the considerations. [German] Vor dem Hintergrund einer drohenden globalen Erwaermung, die eine Reduktion der klimawirksamen Treibhausgase bundesweit um 20 % bis 2010 und um 80 % bis 2050 noetig macht, sind Klimaschutzaktivitaeten auf einer lokalen oder kommunalen Ebene in mehreren Dimensionen sinnvoll. Den tatsaechlichen, bezifferbaren Reduktionen von CO2, die hier geleistet werden, gesellt sich ein ''vorbildhafter'' Druck auf eine stockende internationale Klimapolitik hinzu, ebenso leistet die ''Klimaschutzpolitik von unten'' einer nachhaltigen Entwicklung der lokalen Ebene durch Lerneffekte Vorschub. Unter anderem aus

  4. New approaches for the reliability-oriented structural optimization considering time-variant aspects; Neue Ansaetze fuer die zuverlaessigkeitsorientierte Strukturoptimierung unter Beachtung zeitvarianter Aspekte

    Energy Technology Data Exchange (ETDEWEB)

    Kuschel, N.


    The optimization of structures with respect to cost, weight or performance is a well-known application of the nonlinear optimization. However reliability-based structural optimization has been subject of only very few studies. The approaches suggested up to now have been unsatisfactory regarding general possibility of application or easy handling by user. The objective of this thesis is the development of general approaches to solve both optimization problems, the minimization of cost with respect to constraint reliabilty and the maximization of reliability under cost constraint. The extented approach of an one-level-method will be introduced in detail for the time-invariant problems. Here, the reliability of the sturcture will be analysed in the framework of the First-Order-Reliability-Method (FORM). The use of time-variant reliability analysis is necessary for a realistic modelling of many practical problems. Therefore several generalizations of the new approaches will be derived for the time-variant reliability-based structural optimization. Some important properties of the optimization problems are proved. In addition some interesting extensions of the one-level-method, for example the cost optimization of structural series systems and the cost optimization in the frame of the Second-Order-Reliabiity-Method (SORM), are presented in the thesis. (orig.) [German] Die Optimierung von Tragwerken im Hinblick auf die Kosten, das Gewicht oder die Gestalt ist eine sehr bekannte Anwendung der nichtlinearen Optimierung. Die zuverlaessigkeitsorientierte Strukturoptimierung wurde dagegen weit seltener untersucht. Die bisher vorgeschlagenen Ansaetze koennen bezueglich ihrer allgemeinen Verwendbarkeit oder ihrer nutzerfreundlichen Handhabung nicht befriedigen. Das Ziel der vorliegenden Arbeit ist nun die Entwicklung allgemeiner Ansaetze zur Loesung der beiden Optimierungsprobleme, einer Kostenminimierung unter Zuverlaessigkeitsrestriktionen und einer

  5. Numerically efficient simulation of multi-vaporator air conditioners in highly dynamic boundary conditions; Numerisch effiziente Simulation von Mehrverdampfer-Klimaanlagen unter hochdynamischen Randbedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Schulze, Christian; Kaiser, Christian [TLK-Thermo GmbH, Braunschweig (Germany); Tegethoff, Wilhelm; Koehler, Juergen [TU Braunschweig (Germany). Inst. fuer Thermodynamik


    In the development of physically based models for dynamic simulations of cycle processes, a good equilibrium must be chosen between the degree of detailing and the speed of calculation. Dynamic modelling of mult-evaporator air conditioners is a special challenge as the interaction of several heat transfer fluides at one pressure level may result in numerically challenging effects like reflux. The contribution goes into the simulation of the heat transfer fluids in such systems in highly dynamic boundary conditions, e.g. after shutoff of the compressor. There are different modelling methods, e.g. finite volume, moving boundary, or finite element analysis. The methods are presented and evaluated. For the 1-D finite volume method, various established simplification strategies are presented that may enhance numerical efficiency. It is also shown that the equation system will not always be solvable with these strategies, and an approach to ensure solvability is presented. The new approach is illustrated by the example of a multi-evaporator bus air conditioner. [German] Bei der Erstellung von physikalisch basierten Modellen fuer die dynamische Simulation von Kreisprozessen muss ein gutes Gleichgewicht zwischen Detaillierungsgrad und Rechengeschwindigkeit gewaehlt werden: Das Modell muss die Realitaet hinreichend genau abbilden, im Gegenzug jedoch auch innerhalb eines angemessenen Zeitraums Ergebnisse liefern sowie in allen Betriebszustaenden loesbar sein. Die dynamische Modellierung von Mehrverdampfer-Klimaanlagen stellt im Vergleich zu einfachen Kreisprozessen eine besondere Herausforderung dar, da in diesen Systemen die Interaktion mehrerer Waermeuebertrager auf einem Druckniveau zu numerisch herausfordernden Effekten wie z. B. Rueckstroemung fuehren kann. Dieser Beitrag beschaeftigt sich mit der Simulation der Waermeuebertrager in diesen Systemen unter hochdynamischen Randbedingungen wie z. B. einer Abschaltung des Verdichters. Zur Modellierung der Waermeuebertrager

  6. Alkali release and sorption during combustion and gasification of coal under pressure; Freisetzung und Einbindung von Alkalien bei der Verbrennung und Vergasung von Kohle unter Druck

    Energy Technology Data Exchange (ETDEWEB)

    Steffin, C.R.; Wanzl, W.; Heek, K.H. van [DMT-Gesellschaft fuer Forschung und Pruefung mbH, Essen (Germany). Geschaeftsbereich FuelTec - Kokerei- und Brennstofftechnik


    Investigations and model calculations have shown that water-soluble alkaline substances (in German coal, mostly NaCl) are released by a desorption process rather than a purely physical evaporation process. Sorption takes place in the presence of reactive oxygen species (H{sub 2}O or O{sub 2}), but only the reaction with water vapour is applicable to technical processes. Soorption is preferred to desorption at low temperatures both kinetically and thermodynamically. Metakaolin has a high alkali sorption capacity and potential depending significantly on the chemical properties of the clay mineral. With increasing basicity, the sorption potential decreases drastically, which has a significant effect in terms of fuel selection and mode of operation of the process of coal dust combustion under pressure. A fuel with low alkali emissions would be a coal grade with a small fraction of water-soluble alkalis and a high fraction of acid clay minerals. Intensive scrubbing in a wet chemical treatment stage may serve to reduce the water-soluble alkalis. [Deutsch] Die Untersuchungen und Modellrechnungen haben gezeigt, dass die Freisetzung der wasserloeslichen Alkalien, was in deutschen Steinkohlen hauptsaechlich NaCl ist, ein Desorptionsmechanismus zugrunde liegt und die Vorstellung eines rein physikalischen Verdampfungsprozesses verworfen werden muss. Die Einbindung der Alkalien findet unter Teilnahme reaktiver Sauerstoffspezies (H{sub 2}O oder O{sub 2}) statt, wobei aber nur die Reaktion mit Wasserdampf auch auf technische Prozesse uebertragbar ist. Die Einbindung ist gegenueber der Desorption bei niedrigen Temperaturen kinetisch und thermodynamisch bevorzugt. Metakaolin besitzt eine hohe Kapazitaet und ein hohes Potential zur Einbindung von Alkalien, wobei die Faehigkeit zur Einbindung ganz entscheidend von den chemischen Eigenschaften des Tonminerals abhaengig ist. Mit steigender Basizitaet nimmt das Einbindungspotential der Mineralsubstanz drastisch ab. Das hat auch

  7. Investigations on radionuclide release and on the corrosion behaviour of spent fuels from research reactors under disposal conditions. Final report; Untersuchungen zur Radionuklidfreisetzung und zum Korrosionsverhalten von bestrahltem Kernbrennstoff aus Forschungsreaktoren unter Endlagerbeedingungen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Bruecher, H.; Curtius, H.; Fachinger, J.; Kaiser, G.; Mazeina, L.; Nau, K.


    From the present report 'Untersuchungen zur Radionuklidfreisetzung und zum Korrosionsverhalten von bestrahltem Kernbrennstoff aus Forschungsreaktoren unter Endlagerbedingungen' with the code number FKZ 9108 carried out in the time periode 01.06.1998 till 30.11.2001 the following results can be withdrawn: U/Al-RR-fuel elements corroded slowly in granite water (Grimsel-West) at 90 C under anaerobic conditions. For a complete dissolution of the fuel element a time period of 10{sup 3} years is assumed according to present conservative results. In salt brines, especially in magnesium chloride rich brines the corrosion rate is high. Addition of GGG40 (basic material of the fuel element container with iron as main element) had an acceleration effect. A complete dissolution of the fuel is achieved within a couple of months. Under aerobic and under anaerobic conditions the bulk of released radionuclides were fixed by the corrosion products formed (secondary phases). The actinides were mobilised by variation of the ionic strength of the leaching solution. This process can be explained by phase conversion reactions within the secondary phases. Secondary phases formed by corrosion of a non-irradiated U/Al-RR-fuel element, were analysed and hydrotalcites were identified as phase components. This result justifies the assumption, that hydrotalcites are components of the corrosion products from irradiated fuels. To clarify the questions which bindings exist between radionuclides and secondary phases, sorption experiments were performed. The sorption experiments were performed in salt brines and in granite water using repository relevant radionuclides and minerals which are considered to represent thermodynamic final components of the secondary phases. Pu sorbed as cationic species quantitatively and the binding is covalent. In granite water the same behaviour was found for Am. (orig.) [German] Aus dem vorliegenden Bericht 'Untersuchungen zur Radionuklidfreisetzung

  8. Assimilation of carbon and transport of assimilates in coniferous trees under the influence of airborne pollutants. Photosynthesis rate and assimilate distribution in young fir trees (Picea abies [L.]Karst.) under controlled exposure to ozone. Final report; Assimilation von Kohlenstoff und der Assimilattransport in Coniferen unter dem Einfluss von Luftschadstoffen. Photosyntheseleistung und Assimilatverteilung in jungen Fichten (Picea abies [L.]Karst.) unter kontrollierter Ozon-Exposition. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Willenbrink, J.; Schatten, T.


    In this project, effects of long-term, low-level ozone exposure on CO{sub 2}fixation and assimilate partitioning in 4-years spruces old were studied. 1. In October, ozone exposure resulted in a 40% decrease of photosynthesis. Chlorophyll content was not altered. Depression of net photosynthesis by ozone exposure fluctuates. At least partly, this depression of net photosynthesis might reflect an increase of respiration. After 6 weeks of ozone exposure, both stomatal conductivity and transpiration seemed to be lowered. However, internal [CO{sub 2}] and water use efficiency were not significantly altered. 2. Compared with the control, needles of ozone-exposed plants exhibited a shift of the {sup 14}C-ratio of {beta}-carotene/xanthophylls which effect faded away with the season (October). 3. In August and October, translocation of {sup 14}C-assimilates from the {sup 14}C-fed source leaves seems to be impeded by ozone exposure. 4. No O{sub 3}-related differences were found for the lipophilic as well as for the fractions of organic or amino acids. For the sink tissues (twig, stem, root), less label was found in non-starch residue in August (main growth season), in O{sub 3}-exposed plants. 5. Starch and sugar content of cortex, cambium, xylem, and of the root follow annual cycles. Under ozon exposure, levels of {sup 14}C-starch and {sup 14}C-sucrose are lowered whereas in the needles, build-up of {sup 14}C-starch was detected. 6. Obviously, long-term low-level ozone affects allocation of assimilates. Secondary effects on chloroplasts by the treatment cannot be excluded. (orig./UWA) [Deutsch] Unter Labor- bzw. Gewaechshausbedingungen wurde der Einfluss einer kontrollierten Ozonexposition auf die Photosyntheseleistung und die Assimilatverteilung in 4-jaehrigen Fichten untersucht. 1. Unter Ozon-Exposition wird die Netto-Photosyntheserate in den jungen Nadeln zum Herbst hin reduziert, waehrend der Chlorophyllgehalt unbeeinflusst bleibt. Die Photosyntheserate der jungen Nadeln

  9. Radiology in managed care environment: Opportunities for cost savings in an HMO; Radiologie unter Managed-Care-Bedingungen. Einsparpotenziale aus der Sicht einer Krankenversicherung in den USA

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, C. [Universitaet Kiel (Germany). Klinik fuer Allgemeine Chirurgie und Thoraxchirurgie; Mohr, A. [University of California, San Francisco (United States). Dept. of Radiology; Universitaet Kiel (Germany). Klinik fuer Radiologische Diagnostik; Moeller, J. [Universitaet Bielefeld (Germany). Fakultaet fuer Gesundheitswissenschaften; Levin-Scherz, J. [Tufts Healthplan, Boston (United States); Heller, M. [Universitaet Kiel (Germany). Klinik fuer Radiologische Diagnostik


    : Darstellung des Einsparungspotenzials, welches einer effizienten Nutzung von Radiologieleistungen in einer Health-Maintenance-Organisation (HMO) innewohnt, und der zur Realisierung verwendeten Managed-Care-Techniken. Material und Methoden: Im Rahmen eines Projektes zur Kostenreduktion an einer HMO in den USA wurden die Ursachen fuer gestiegene Ausgaben im Bereich Radiologie (''Radiology-Utilisation-Line'') untersucht. Durch umfassende Datenanalysen, Ueberpruefungen der Kodierlogik, Interviews mit Angestellten sowie kritische Beleuchtung der Vertragsbeziehungen zu Leistungserbringern konnte das Einsparpotenzial beziffert werden. Saemtliche Daten wurden in Microsoft Excel {sup trademark} dokumentiert und unter Verwendung von SPSS 8.0 (Statistical Package for the Social Sciences) fuer Windows {sup trademark} mithilfe nicht-parametrischer Tests ausgewertet. Ergebnisse: Hauptursache war eine verstaerkte Verordnung von Radiologieleistungen pro versichertem Patienten, wobei die Kosten pro Untersuchung annaehernd gleich blieben. Ein wesentlicher Kostenhebel lag in der Verbesserung des elektronischen Abrechnungssystems, welches fuer eine Ueberbezahlung von Leistungen verantwortlich war. Die Gestaltung der vertraglichen Risikovereinbarungen zwischen Versicherer und Leistungserbringer sowie die Qualitaet der Managementstrukturen innerhalb der radiologischen Einrichtungen hatten einen signifikanten Einfluss auf deren Wirtschaftlichkeit. Ferner konnte nachgewiesen werden, dass Aerzte mit eigenen Roentgenanlagen bis zu 4-5-mal mehr Untersuchungen veranlassen als Aerzte ohne eigene Roentgenausstattung und so einen erheblichen Kostentreiber darstellen. (orig.)

  10. Receptor imaging of schizophrenic patients under treatment with typical and atypical neuroleptics; Nuklearmedizinische Rezeptordiagnostik bei schizophrenen Patienten unter Therapie mit typischen und atypischen Neuroleptika

    Energy Technology Data Exchange (ETDEWEB)

    Dresel, S.; Tatsch, K. [Klinik und Poliklinik fuer Nuklearmedizin der Ludwig-Maximilians-Univ. Muenchen (Germany); Meisenzahl, E. [Psychiatrische Klinik der Ludwig-Maximilians-Univ. Muenchen (Germany); Scherer, J. [Bezirkskrankenhaus Haar (Germany)


    -motorische Nebenwirkungen hervorzurufen. Die Besetzung postsynaptischer Dopamin-D2-Rezeptoren wird als ein wesentlicher Aspekt der antipsychotischen Wirksamkeit der Neuroleptika angesehen. Diese koennen nuklearmedizinisch durch [I-123]IBZM-SPECT dargestellt werden. Fuer das typische Neuroleptikum Haloperidol wurde eine dosisabhaengige, exponentielle Besetzung der Dopamin-D2-Rezeptoren nachgewiesen. Ab einem Schwellenwert des spezifischen Bindungsindex von 0,4 (Norm: >0,95) zeigten mit einer Ausnahme alle untersuchten Patienten extrapyramidal-motorische Nebenwirkungen. Auch unter Therapie mit dem atypischen Neuroleptikum Clozapin ergab sich eine exponentielle Dosis-Wirkungsbeziehung mit jedoch einem deutlich flacheren Kurvenverlauf im Vergleich zu Haloperidol. Extrapyramidalmotorische Nebenwirkungen traten bei diesen Patienten nicht auf. Neuere, als atypisch eingestufte Neuroleptika wie Risperidon und Olanzapin zeigten ebenfalls eine exponenzielle Dosis-Wirkungsbeziehung, wobei deren Kurvenverlaeufe zwischen denen von Haloperidol und Clozapin lagen. Extrapyramidal-motorische Nebenwirkungen traten bei den letzteren Neuroleptika seltener als bei Haloperidol, bei Olanzapin nur bei einem Patienten in unserem eigenen Patientengut auf. Das pharmakologische Profil atypischer Neuroleptika zeichnet sich neben der Bindung an die postsynaptischen Dopamin-D2-Rezeptoren auch durch Bindung an Rezeptoren diverser anderer Neurotransmittersysteme, insbesondere des serotonergen Systems, aus. Somit ist wahrscheinlich, dass die niedrigere Inzidenz fuer extrapyramidal-motorische Nebenwirkungen bei atypischen Neuroleptika durch (orig.)

  11. Percolation experiments to determine fluid-matrix interaction (with particular regard to pretreatment of the drill core); Kerndurchstroemungsversuche zur Ermittlung von Fluid-Matrix-Wechselwirkungen (unter besonderer Beruecksichtigung der Kernvorbehandlung)

    Energy Technology Data Exchange (ETDEWEB)

    Martin, M; Seibt, A [TU Bergakademie Freiberg (Germany). Inst. fuer Bohrtechnik und Fluidbergbau; Hoth, P [GeoForschungsZentrum Potsdam (Germany)


    The injection of fluids into sandstone reservoirs leads to interactions between these waters, the reservoir rocks, and the formation fluids. Estimations about possible permeability reducing processes caused by these interactions are therefore of great importance for the exploitation of sandstone aquifers as geothermal reservoirs. Percolation experiments under in situ conditions with core samples from North German geothermal boreholes were done in order to investigate these fluid-rock interactions. (orig./AKF) [Deutsch] Die Injektion von Fluiden in Aquiferspeicher fuehrt zu Wechselwirkungen zwischen dem Speichergestein, den Formationsfluiden und den injizierten Loesungen. Fuer die Bewirtschaftung der Speicher sind insbesondere Kenntnisse ueber moegliche Permeabilitaetsreduzierungen durch diese Wechselwirkungen von Bedeutung. Mit Hilfe von Kern-Durchstroemungsexperimenten, durchgefuehrt unter lagerstaettenaehnlichen Bedingungen mit Original- bzw. modifizierten Fluiden, wurde daher das Durchstroemungsverhalten von unterschiedlich ausgebildeten Reservoirsandsteinen aus norddeutschen Geothermiebohrungen untersucht. (orig./AKF)

  12. The influence of N-acetylcysteine on the measurement of prothrombin time and activated partial thromboplastin time in healthy subjects

    DEFF Research Database (Denmark)

    Jepsen, S; Hansen, A B


    mg kg-1 h-1 for 32 h in six male subjects. The intrinsic, extrinsic and common pathway of coagulation were monitored with activated partial thromboplastin time (APTT), and prothrombin time, respectively. In addition, the extrinsic coagulation pathway was monitored with the clotting activity of single...... factors II, VII, and X. No effect on the intrinsic coagulation pathway was observed. There was a significant and rapid decrease in prothrombin time. Coagulation factors II, VII and X, the three components of prothrombin time, decreased significantly to different degrees. We conclude that infusion of N...

  13. N-acetylcysteine and other preventive measures for contrast-induced nephropathy in the intensive care unit

    NARCIS (Netherlands)

    Schultz, Marcus J.; Baas, Marije C.; van der Sluijs, Hans P.; Stamkot, G. André; Smit, Watske


    The increase in diagnostic imaging procedures that require infusion of intravenous radiographic contrast has led to a parallel increase in the incidence of contrast-induced nephropathy (CIN). Since CIN accounts for a significant increase of hospital-acquired renal failure, length of stay and

  14. Ghrelin and NUCB2/Nesfatin-1 expression in unilateral testicular torsion-induced rats with and without N-acetylcysteine. (United States)

    Sarac, M; Bakal, U; Tartar, T; Kuloglu, T; Yardim, M; Artas, G; Aydin, S; Kazez, A


    Testicular torsion (TT) is a common urological problem in the field of pediatric surgery. The degree and duration of torsion determines the degree of testicular damage; however, its effects on the expression of octanoylated ghrelin and nucleobindin 2 (NUCB2) /nesfatin-1 synthetized from testicular tissue remain unclear. We explored the effects of experimentally induced unilateral TT on serum and contralateral testicular tissue ghrelin and NUCB2/nesfatin-1 levels, and determined whether N-acetyl cysteine (NAS) treatment had any effects on their expression. A total of 42 Wistar Albino strain rats were divided into 7 groups: Group (G) I control, GII sham, GIII 12-hour torsion, GIV 12-hour torsion + detorsion + 100 mg/kg NAS, GV 24-hour torsion, GVI 24-hour torsion + detorsion + 100 mg/kg NAS, and GVII 100 mg/kg NAS. Octanoylated ghrelin and NUCB2/nesfatin-1 concentrations were evaluated in serum using the ELISA method and in testicular tissue with immunohistochemical methods. Immunoreactivity of octanoylated ghrelin significantly increased in GI compared to GIII, GV, and GVI (p<0.05). NUCB2/nesfatin-1 immunoreactivity increased in GV and GVIII relative to GI (p<0.05). In the 12-hour torsion group, a significant decrease in octanoylated ghrelin levels with NAS treatment was observed; however, in the 24-hour torsion group, a significant decrease was not observed. In the 12-hour torsion + NAS treatment group, a significant change was not observed in NUCB2/nesfatin-1 expression. Following 24-hour torsion, an increase in NUCB2/nesfatin-1 levels was observed, and NAS treatment did not reverse this increase. It was determined that increases in the expression of octanoylated ghrelin and NUCB2/nesfatin-1, the latter of which was a result of TT, reflect damage in this tissue. Importantly, NAS treatment could prevent this damage. Thus, there may be a clinical application for the combined use of NAS and octanoylated ghrelin in preventing TT-related infertility.

  15. Does N-acetylcysteine have an effect on acetylcholine-induced contractions and histopathological changes on isolated rat ileum?

    International Nuclear Information System (INIS)

    Koc, E.; Yavuzer, Sema A.; Ocakcioglu, B.; Ergun, A.; Can, B.; Saran, Y.


    Objective was to investigate the action of N-acetyllcysteine (NAC) on rat isolated ileal contractility and to determine the effects of NAC on histopathological changes on ileal tissue. The study took place at the Faculty of Medicine, Ankara University, Ankara, Turkey, January 2003. Adult Wistar rats were used in all experiments. Two groups were designed. The experimental group, to which NAC 0.5 g/Kg/day was administered orally by adding to their water for 7 days, and the control group to which only saline was administered. At the end of experimental periods, one cm pieces of terminal ileum segments were removed for testing ileal contractility, and one cm pieces of ileum segments were removed for histopathological experiments. The acetylcholine (ACh)-induced contraction was recorded, and the ileal tissue examined using light and electron microscopic techniques for histopathological changes. The average peak amplitude of Ach-induced contraction recorded in standard tyrode solution of the experimental group was decreased significantly when compared to the control group in standard and calcium-free tyrode solution. On histopathological findings, there were swollen mitochondria with disturbed cristae in ileal muscle. Our data suggest that the NAC in the present experiment decreased the ACh-induced contractility on rat-isolated ileum. (author)

  16. Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats


    Martinez, Paula Felippe [UNESP; Bonomo, Camila [UNESP; Guizoni, Daniele Mendes [UNESP; Oliveira Junior, Silvio Assis [UNESP; Damatto, Ricardo Luiz [UNESP; Cezar, Marcelo Diarcadia Mariano [UNESP; Lima, Aline Regina Ruiz [UNESP; Pagan, Luana Urbano [UNESP; Seiva, Fabio Rodrigues; Fernandes, Denise Castro; Laurindo, Francisco Rafael Martins; Novelli, Ethel Lourenzi Barbosa [UNESP; Matsubara, Luiz Shiguero [UNESP; Zornoff, Leonardo Antonio Mamede [UNESP; Okoshi, Katashi [UNESP


    Background: Chronic heart failure is characterized by decreased exercise capacity with early exacerbation of fatigue and dyspnea. Intrinsic skeletal muscle abnormalities can play a role in exercise intolerance. Causal or contributing factors responsible for muscle alterations have not been completely defined. This study evaluated skeletal muscle oxidative stress and NADPH oxidase activity in rats with myocardial infarction (MI) induced heart failure. Methods and Results: Four months after MI,...

  17. MR-imaging of the breast at 0.5 Tesla: menstrual-cycle dependency of parenchymal contrast enhancement in healthy volunteers with oral contraceptive use?; MR-Mammographie bei 0,5 Tesla: Menstruationszyklusabhaengigkeit der Kontrastmittelanreicherung unter hormoneller Kontrazeption?

    Energy Technology Data Exchange (ETDEWEB)

    Lorenzen, J.; Welger, J.; Krupski, G.; Adam, G. [Klinik und Poliklinik fuer Diagnostische und Interventionelle Radiologie, Universitaetsklinikum Hamburg-Eppendorf (Germany); Lisboa, B.W. [Klinik und Poliklinik fuer Frauenheilkunde und Geburtshilfe, Universitaetsklinikum Hamburg-Eppendorf (Germany)


    Introduction: To evaluate changes of contrast medium enhancement of the breast parenchyma due to menstrual cycle in healthy volunteers with oral contraceptive use in MR-imaging of the breast. Material and Methods: 15 healthy volunteers (age: 22 - 36, mean 28,2) without breast disease were examined two times during one menstrual cycle (days 7 - 14 and days 21 - 2). Two volunteers were examined only in the second part of the cycle (days 21 - 2). All volunteers used oral contraceptives for more than 6 month continuously. Examinations were performed with a 0,5 T magnet (dynamic 3D-gradient echo protocol with subtraction postprocessing). We evaluated the number of enhancing foci and the parenchymal contrast medium enhancement during the different phases of the cycle by region of interest. Results: Only a total of two enhancing foci were found in 2 of 17 volunteers. Time/signal intensity diagrams in these both cases were not suspicious (< 80% initial signal increase after of contrast medium injection, no wash-out phenomenon) and sonography of the breast in these two cases was inconspicuous. Contrast medium enhancement of breast parenchyma in cycle days 7 - 14 (mean enhancement: 0.12 - 0.26, minutes 1 - 9 p.i.) was not significantly different (p = 0.2209; Wilcoxon signed rank test) from cycle days 21 - 2 (mean: 0.13 - 0.32). Conclusion: Menstrual cycle dependency of parenchymal contrast medium enhancement seems to be of minor relevance for premenopausal women with use of oral contraceptives. (orig.) [German] Fragestellung: Untersuchung der Kontrastmittelaufnahme des Brustparenchyms in Abhaengigkeit vom Menstruationszyklus bei gesunden Probandinnen unter oraler Kontrazeption. Material und Methode: Bei 15 gesunden Probandinnen ohne Brusterkrankung in der Anamnese wurde eine MR-Mammographie zweimal waehrend eines Menstruationszyklus durchgefuehrt (Zyklustag: 7 - 14 und 21 - 2). Bei zwei weiteren Probandinnen erfolgte die MR-Mammographie nur in der zweiten Zyklushaelfte

  18. The value of magnetic resonance imaging (MRI) in the treatment planning of vertebral metastasis considering economic aspects. A cost benefit-analysis; Die Wertigkeit der Magnetresonanztomographie (MRT) unter oekonomischen Aspekten bei der Bestrahlungsplanung von Wirbelkoerpermetastasen. Eine Kosten-Nutzen-Analyse

    Energy Technology Data Exchange (ETDEWEB)

    Prott, F.J.; Schlehuber, E.; Scharding, B.J.; Rinast, E. [Strahlentherapie Wiesbaden, St.-Josefs-Hospital (Germany); Micke, O. [Klinik und Poliklinik fuer Strahlentherapie - Radioonkologie, Universitaetsklinikum Muenster (Germany)


    Is magnetic resonance imaging (MRI) based target volume definition for treatment planning of vertebral metastasis effective under economic considerations.From 1994 to 1999, a total of 137 patients with bone metastases affecting the vertebral column underwent MRI of the cercival, thoracic, or lumbar spine for the treatment planning of palliative radiation therapy. The following radiation treatment consisted in a irradiation of the affected vertebral region up to a total dose of 30-40 Gy.The cost calculation for radiotherapy and magnetic resonance tomography was done using the common tariff model (EBM) of the German Health Insurances.In 73% of patients (101 patients), magnetic resonance imaging resulted in marked corrections of the irradiation fields which would have resulted in the necessity of treatment for recurrence in the case of treatment planning without MRI.Consequently, the higher cost of MRI of 345.00 DEM (176,40 EUR) lead to a saving of 497.00 DEM (254,11 EUR) compared to a recurrence treatment of 10 fractions and of 1,428.00 DEM (730,12 EUR) compared to 20 fractions. The transport expenses for the second treatment could be saved as well.Even under economic considerations MRI is effective. (orig.) [German] Ist eine MRT-gestuetzte Zielvolumendefinition bei der Strahlentherapie von Knochenmetastasen ein sinnvolles Vorgehen unter oekonomischen Gesichtspunkten?1994-1999 wurde bei 137 Patienten mit einem ossaer metastasierenden Tumor und Befall der Wirbelsaeule eine Magnetresonanztomographie (MRT) zur Planung einer palliativen Radiatio durchgefuehrt. Die nachfolgende Strahlentherapie bestand aus einer Radiatio des betroffenen Wirbelkoerperabschnitts bis zu einer Gesamtherddosis von 30-40 Gy. Fuer die Berechnung der Kosten wurde der einheitliche Bewertungsmassstab der Deutschen Krankenkassen zugrunde gelegt.Aufgrund der MRT-Untersuchungen wurde bei 73% der Patienten eine Veraenderung des Bestrahlungsfeldes vorgenommen, die im Falle einer nicht MRT

  19. Powertrain definitions for Opel's brand sound considering the vehicle portfolio; Gestaltung des Powertrains fuer den Opel-Brand-Sound unter Beruecksichtigung der unterschiedlichen Positionierung der Fahrzeugpalette

    Energy Technology Data Exchange (ETDEWEB)

    Bernhard, U.; Herrmann, G.; Schmidt, G.O. [Adam Opel AG, Ruesselsheim (Germany)


    The change of value orientation in Western Europe lead to an increase of emotions buying a car. In addition to classic factors like design, today the carmakers use elements of emotion like brand sound to inspire the customers. On the one hand the job of the acoustic engineers is to meet the acoustic expectations depending on driving conditions. On the other hand there are to develop both combining elements over the whole portfolio and elements which allow a defined position of a carline-powertrain combination in the market. (orig.) [German] Im Zuge der Wandlung der westlichen Gesellschaften spielen Emotionen beim Kauf eines Automobils eine immer staerkere Rolle. Zur Unterstuetzung klassischer Mittel wie dem Design werden heute weitere emotionalisierende Elemente zur Begeisterung der Kunden eingesetzt, unter anderem auch das Klangbild eines Fahrzeugs. Dabei liegt die Aufgabe einerseits darin, der unterschiedlichen Erwartungshaltung in Abhaengigkeit vom Fahrzustand des Fahrzeugs gerecht zu werden. Andererseits die Gestaltung des Klangbildes so durchzufuehren, dass innerhalb des Portfolios sowohl verbindende Elemente als markentypisch zu finden sind als auch der Marktpositionierung gerecht werdende Elemente der einzelnen Fahrzeugtypen zu generieren. (orig.)

  20. Comparison calculation/experiment on the load case ``shutdown of TH high pressure pumps under consideration of fluid structure interaction``; Vergleich Rechnung/Messung zum Lastfall ``Abschaltung der TH-Hochdruckpumpen unter Beruecksichtigung der Fluid-Struktur-Wechselwirkung``

    Energy Technology Data Exchange (ETDEWEB)

    Erath, W.; Nowotny, B.; Maetz, J. [KED, Rodenbach (Germany)


    Measurements of an experiment in a pipe system with pump shutdown and valve closing have been performed in the nuclear power plant KRB II. Comparative calculations of fluid and structure including interaction show an excellent agreement with the measured results. Theory and implementation of the fluid/structure interaction and the results of the comparison are described. It turns out that the consideration of the fluid/structure interaction is mostly a significant increase of the effective structural damping. (orig.) [Deutsch] Es wurden Messungen am nuklearen Nachkuehlsystem des Kernkraftwerks Gundremmingen (KRB II) bei einem Versuche mit Pumpenabschalten und Ventilschliessen durchgefuehrt. Vergleichsrechnungen der Fluid-Strukturdynamik unter echter Beruecksichtigung der Wechselwirkung ergaben eine ausgezeichnete Uebereinstimmung der Rechnung mit den Messungen. Es werden Theorie und Implementierung der Koppelung der Fluid- und Struktur-Berechnungen sowie die Vergleiche von Messung und Rechnung beschrieben. Es ergibt sich, dass die Beruecksichtigung der Wechselwirkung notwendig ist zur genaueren Berechnung von `weichen` Rohrleitungsystemen. Eine wichtige Folge der Wechselwirkung ist meist eine deutliche Erhoehung der effektiven Strukturdaempfung. (orig.)

  1. Optimisation of a truck diesel engine with a view to environmental pollution. Interstate research project. Final report; Optimierung eines mit Rapsoelmethylester betriebenen Nutzfahrzeug-Dieselmotors unter besonderer Beruecksichtigung der Umweltvertraeglichkeit. Laenderuebergreifendes Verbundforschungsvorhaben. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Hattingen, U.; Adt, H.U. [Kaiserslautern Univ. (Germany). Arbeitsgruppe Verbrennungskraftmaschinen; Sauer, B.; Ruhl, C.; Huber, M. [Kaiserslautern Univ. (Germany). Lehrstuhl fuer Maschinenelemente und Getriebetechnik; Regitz, M.; Weber, B. [Kaiserslautern Univ. (Germany). Lehrstuhl fuer Organische Chemie; Bereswill, S. [Motoren-Werke Mannheim AG (Germany); Luther, R.; Acker, I. [Fuchs Mineraloelwerke GmbH, Eschweiler (Germany)


    Detailed rheological analyses of lubricants are carried out with a view to the temperature dependence of the viscosity functions. Two sliding bearings of similar design but made of different materials were tested on a test bench after lubrication with an oil of the SAE grade 10W-40 diluted with increasing concentrations of rapeseed oil methyl ester. The results were analyzed and compared with the bearings characteristics calculated according to DIN 31652. The measurements were found to be in good agreement with calculated values. [German] Im Rahmen dieses Projektes wurden zunaechst detaillierte rheologische Untersuchungen der Schmierstoffeigenschaften unter Beruecksichtigung der Schmieroelverduennung mit RME und DK vorgenommen und die Temperaturabhaengigkeit der jeweiligen Viskositaetsfunktion ermittelt. Auf einem geeigneten Gleitlagerpruefstand wurden zwei bezueglich der Motorenhauptlager geometrisch aehnliche Lager unterschiedlichen Werkstoffes untersucht. Die Schmierung erfolgte dabei mit einem Motorenoel der SAE-Klasse 10W-40, welches zuvor jeweils mit einem im Verlauf der Versuchsreihe ansteigenden Anteil an Rapsoelmethylester verduennt wurde. Die Messergebnisse wurden ausgewertet und den nach DIN 31652 berechneten Lagerkennzahlen gegenuebergestellt. Danach ergeben sich fuer die gemessenen Lagerkenngroessen qualitativ aehnliche Abhaengigkeiten von der Schmieroelverduennung wie berechnet. (orig.)

  2. Cross-sectoral modeling and optimization of a future German energy system, taking energy efficiency measures into account in the building sector; Sektoruebergreifende Modellierung und Optimierung eines zukuenftigen deutschen Energiesystems unter Beruecksichtigung von Energieeffizienzmassnahmen im Gebaeudesektor

    Energy Technology Data Exchange (ETDEWEB)

    Palzer, Andreas


    With the aim of reducing greenhouse gas emissions, comprehensive climate protection measures have already been adopted both nationally and internationally. This raises the question of how economically and ecologically useful system infrastructure looks, which at the same time ensures the supply reliability of all consumers. The regenerative energy model (REMod) presented in this book has been developed to provide answers. The sectors electricity, heat, transport and industry are considered for the first time simultaneous in an energy system model. In particular, in order to satisfy the criterion of reliability of supply, the model calculates the energy flows in hourly resolution for the period from today (2015) to 2050. The system is optimized with regard to minimum overall costs and under the boundary condition that a maximum set quantity of permitted greenhouse gas emissions is not exceed. On the example of Germany (REMod-D), the results show that, in particular, the interaction of the sectors can lead to strong differences in the design of the system infrastructure. [German] Mit dem Ziel den Ausstoss der Treibhausgase zu reduzieren, wurden bereits national wie international umfangreiche Klimaschutzmassnahmen verabschiedet. Hieraus ergibt sich die Frage wie eine oekonomisch und oekologisch sinnvolle Systeminfrastruktur aussieht, die gleichzeitig die Versorgungssicherheit aller Verbraucher gewaehrleistet. Das in diesem Buch vorgestellte Regenerative Energien Modell (REMod) wurde entwickelt um hierauf Antworten zu liefern. Beruecksichtigt werden erstmalig in einem Energiesystemmodell die Sektoren Strom, Waerme, Verkehr und Industrie gleichzeitig. Insbesondere um dem Kriterium der Versorgungssicherheit gerecht zu werden, berechnet das Modell die Energiefluesse in stuendlicher Aufloesung fuer den Zeitraum von heute (2015) bis 2050. Optimiert wird das System hinsichtlich minimaler Gesamtkosten und unter der Randbedingung, dass eine maximal vorgegebene Menge erlaubter

  3. Marginal flow in compressor cascades. Influence of gap, cascade geometry, loads and inflow boundary layer. Final report; Randstroemung in Verdichtergittern. Untersuchung der Randstroemung in Verdichtergittern unter dem Einfluss von Spalt, Gittergeometrie, Gitterbelastung und Zustroemgrenzschicht. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Huebner, J


    der Abstroemwinkelverteilung zusammengestellt und mit den Messungen verglichen. Dabei ergab sich eine grosse Streubreite der Korrelationen, die auf eine unterschiedliche Bewertung der Spaltweite und der aerodynamischen Belastung zurueckzufuehren ist. Die haeufig anzutreffende Trennung in einen reinen Spaltverlust und einen von der Spaltstroemung unbeeinflussten Sekundaerverlust erscheint physikalisch nicht sinnvoll. Unter Beruecksichtigung der experimentellen Ergebnisse wurden verbesserte Spaltverlust- und Abstroemwinkelkorrelationen erarbeitet. (orig.)

  4. A laser-based study of kerosine evaporation and -mixing for lean prevaporized combustion at elevated pressure; Lasermesstechnische Untersuchung der Kerosinverdampfung und -mischung fuer die magere Vormischverbrennung unter erhoehtem Druck

    Energy Technology Data Exchange (ETDEWEB)

    Brandt, M.


    The evaporation and mixing of a kerosine spray in the turbulent airstream of a prevaporizer is investigated at conditions prevailing in the combustion chamber of gas turbines. An experiment is described that allows to study an evaporating fuel spray downstream a prefilming airblast atomizer with Phase-Doppler anemometry, laser-induced fluorescence and an infrared light absorption technique. At an air pressure of 9 bars, an air temperature of 750 K, a mean air velocity of 120 m/s and a fuel flow rate of 1 g/s the kerosine spray evaporates completely without autoignition. At this operating condition the parameters air pressure, air temperature and air turbulence are varied. The influence of these parametric variations on the dropsize distribution, the evaporation rate and the concentration profiles of liquid and evaporated fuel is presented and discussed. (orig.) [German] Die Verdampfung und Vermischung eines Kerosinsprays in der turbulenten Luftstroemung eines Vorverdampfers wird unter Bedingungen untersucht, die in Brennkammern fuer Gasturbinen vorherrschen. Ein Experiment wird vorgestellt, welches es erlaubt, ein verdampfendes Kraftstoffspray stromab eines ebenen Luftstromzerstaeubers mit Filmleger mittels der Phasen-Doppler-Anemometrie, Laser-induzierter Fluoreszenz und einer Infrarotabsorptionsmesstechnik zu untersuchen. Bei einem Luftdruck von 9 bar, einer Vorwaermetemperatur der Luft von 750 K, einer mittleren Luftgeschwindigkeit von 120 m/s und einem Kraftstoffmassenstrom von 1 g/s verdampft das Kerosinspray vollstaendig, ohne die Selbstzuendungszeit zu erreichen. Bei dieser Betriebsbedingung werden die Parameter Luftdruck, Lufttemperatur und Turbulenzgrad variiert. Der Einfluss dieser Parameter auf das Tropfengroessenspektrum, den Verdampfungsgrad und die Konzentrationsprofile des fluessigen sowie des verdampften Kraftstoffs wird dargestellt und diskutiert. (orig.)

  5. Materials model for describing the austenite-martensite phase transformation considering transformation-induced plasticity; Ein Materialmodell zur Beschreibung der Austenit-Martensit Phasentransformation unter Beruecksichtigung der transformationsinduzierten Plastizitaet

    Energy Technology Data Exchange (ETDEWEB)

    Oberste-Brandenburg, C.


    In this thesis, a model to describe the austenite martensite transformation was developed. The transformation induced plasticity (TRIP) was taken into consideration. The model can be used to design complex structures. A local examination of the energy and entropy balance at the phase boundary serves as the starting point for the identification of the thermodynamical driving force and the thermodynamic flow. For both, a tensorial description is necessary for a general nonhydrostatically stressed solid. In the second part, a material law for the description of TRIP-Steels was developed based on the values derived in the first part. The different mechanical behavior of the phases, especially the differing yield stresses, was taken into account. The model developed was implemented into the finite element program MARC. Simulations of the material and the structural behavior were performed. The experimentally observed strong dependence of the transformation kinetics on the yield stress of the austenite and the dependence of the orientation of the martensite inclusion on the stress state could be verified. (orig.) [German] Im Rahmen dieser Arbeit wurde ein Materialmodell zur Beschreibung der Austenit-Martensit Phasenumwandlung unter Beruecksichtigung der transformationsinduzierten Plastizitaet (TRIP) entwickelt. Das Modell ist zur Berechnung ausgedehnter Strukturen einsetzbar. Eine lokale Betrachtung der Energie- und Entropiebilanz an der Phasengrenze bildet den Ausgangspunkt zur Identifikation der thermodynamischen Kraft und des thermodynamischen Flusses bei Beschreibung der Transformationskinetik. Fuer beide Groessen muss fuer den allgemein nichthydrostatischen Spannungszustand eine tensorielle Beschreibung verwendet werden. Im zweiten Teil der Arbeit bilden diese Groessen die Basis zur Entwicklung eines Stoffgesetzes zur Beschreibung des TRIP-Phaenomens. Es wird das unterschiedliche mechanische Verhalten der Phasen, insbesondere die stark unterschiedlichen

  6. Implementation of new concepts for space HVAC systems in domestic buildings in consideration of cost, consumption and user comfort. Final report; Umsetzung neuer Konzepte der technischen Gebaeudeausruestung im Wohnungsbau unter Beruecksichtigung von Kosten, Verbraeuchen und Nutzerkomfort. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, F.; Sucic, D.; Wendler, M.


    The goal of this project was the development of a set of tools for the selection of optimal concepts for HVAC-systems in housing buildings. Several factors are considered. They include technical feasibility, costs for investment, maintenance and operation, energy concumption, environmental impacts and user comfort. It is intended with the help of these tools to identify the most important factors which influence energy consumption and to suggest simple and cost effective measures to reduce energy consumption. Measures include improvements of the envelope, changes in the HVAC-system and its operation according to the needs to the inhabitants of the building. This report describes these components of this tool set which were developed in the frame of the project WohnKomfort. It lists data and rules chosen. In addition we report experiences which we gained with an prototypical implementation. This implementation allows load calculations for more than 60.000 different buildings applying EN 802 (only one zone model) as calculation method. It also supports selection of heating systems for such buildings by offering an evaluation according to user specified criteria including cost, environment and comfort. (orig./MM) [Deutsch] Ziel des Vorhabens war die Entwicklung eines Instrumentariums zur Auswahl optimaler Konzepte fuer technische Anlagen in Wohngebaeuden unter Beruecksichtigung der Faktoren technische Machbarkeit, Erstellungs-, Betriebskosten, Energieverbrauch, Umweltbelastung und Nutzerkomfort. Mit Hilfe dieses Instrumentariums soll es moeglich werden, die Haupteinflussfaktoren fuer den Energieverbrauch anzugeben und Vorschlaege fuer einfache und kostenguenstige Massnahmen zu seiner Reduzierung auf der Seite des Gebaeudes (bauliche Massnahmen) und der Anlage (technische Massnahmen, Nutzerverhalten) anzugeben. In diesem Bericht beschreiben wir die im Vorhaben entwickelten Komponenten des Instrumentariums, die zugrundeliegenden Daten und Regeln sowie Erfahrungen, die

  7. Calculations of an unsteady flow through a hydraulic axial turbine with reference to interaction between stator and rotor; Instationaere Berechnung einer hydraulischen Axialturbine unter Beruecksichtigung der Interaktion zwischen Leit- und Laufrad

    Energy Technology Data Exchange (ETDEWEB)

    Bauer, C.


    The objective of this study is the development of an algorithm enabling coupling of nonmatching computational grids to carry out calculations of an unsteady flow through a hydraulic axial turbine with reference to interaction between stator and rotor. The algorithm should offer the possibility to operate the computational grids in a fixed position relative to each other as well as in relative movement. Furthermore, the calculation should be feasible with separate grids in parallel and different frames of reference. Employing selected examples this method is investigated in detail the results are compared with performed measurements. The unsteady numerical examination of the coupling process is carried out with different examples; especially the interaction effects between stator, rotor and draft tube of a hydraulic axial turbine are observed. In addition, the effect of tip clearance of the mean flow is described. Extensive model tests using the axial turbine have been performed at the Institute for Fluid Mechanics and Hydraulic Machinery, IHS. Flow time dependent velocities have been measured with a Laser Doppler Velocimeter placed at midspan of the blading. Periodical changes in static pressure have been recorded at different locations near the wall of the turbine casing. These measurements serve as reference for the comparison with results derived from the unsteady calculations. The confrontation of the time-dependent fluctuations of the flow quantities and the calculation of the efficiency of the turbine resulting from the simulation results allow a comparison in absolute terms. (orig.) [German] Fuer die instationaere Berechnung einer hydraulischen Axialturbine unter Beruecksichtigung der Interaktion zwischen Leit- und Laufrad wird ein Algorithmus zum Koppeln von nichtpassenden Berechnungsnetzen entwickelt. Diese Berechnungsnetze sollen zueinander ortsfest sein oder auch eine Relativbewegung zueinander haben koennen. Sie sollen ausserdem und in unterschiedlichen

  8. A survey of anticoagulation practice among German speaking microsurgeons – Perioperative management of anticoagulant therapy in free flap surgery [Erhebung über die antikoagulatorische Praxis unter deutschsprachigen Mikrochirurgen – Perioperatives Management der antikoagulatorischen Therapie bei freien Lappentransplantaten

    Directory of Open Access Journals (Sweden)

    Jokuszies, Andreas


    Full Text Available [english] Background: Anticoagulation is a crucial element in microsurgery. Although various clinical studies and international surveys have revealed that anticoagulation strategies can vary and result in similar outcomes, anticoagulative regimen are far away from standardization. In Germany and german speaking countries standardized anticoagulation protocols concerning free flap surgery do not exist so far. Methods: To evaluate the current practice of clinics in Germany, Austria and Switzerland with specialization in microsurgery we performed a questionnaire surveying the perioperative regimen of anticoagulant and antiplatelet therapy in free flap surgery. The microsurgeons were interrogated on several anticoagulant, rheologic and antiplatelet medications, their dosage and perioperative frequency of application pre-, intra- and postoperative.Results: The questionnaire revealed that the used antithrombotic and perioperative regimens varied from department to department presumably based on the personal experience of the surgeon. Multiple approaches are used with a wide range of anticoagulants used either alone or in combination, with different intervals of application and different dosages. Conclusion: Therefore consensus meetings should be held in future leading to conduct prospective multicenter studies with formulation of standardized anticoagulative and perioperative protocols in microsurgery reducing flap failure to other than pharmacologic reasons.[german] Hintergrund: Die Antikoagulation stellt ein zentrales Element in der Mikrochirurgie dar. Zahlreiche klinische Studien und internationale Erhebungen zu antikoagulatorischen Strategien weisen eine grosse Varianz bei vergleichbaren Resultaten nach, entbehren jedoch einer Standardisierung. Auch in Deutschland und deutschsprachigen Ländern fehlen bislang standardisierte Regime zur Antikoagulation in der Mikrochirurgie.Methodik: Zur Erhebung der antikoagulatorischen Praxis unter

  9. Ungarn seit der Wahl: Demokratie unter Druck

    NARCIS (Netherlands)

    Freyberg-Inan, A.; Varga, M.


    Die Verfasser zeigen, dass die Orban-Regierung legitime Probleme benennt, wie etwa Ungarns extrem niedrige Beschäftigungsrate, die mit zu den großen wirtschaftliche Schwierigkeiten des Landes beiträgt. Doch packt sie die identifizierten Probleme zu oft auf eine Weise an, die ganz nebenbei dem

  10. Attenuation of ischemia-reperfusion-induced alterations in intracellular Ca2+ in cardiomyocytes from hearts treated with N-acetylcysteine and N-mercaptopropionylglycine. (United States)

    Saini-Chohan, Harjot K; Dhalla, Naranjan S


    This study was undertaken to test whether Ca(2+)-handling abnormalities in cardiomyocytes after ischemia-reperfusion (I/R) are prevented by antioxidants such as N-acetyl L-cysteine (NAC), which is known to reduce oxidative stress by increasing the glutathione redox status, and N-(2-mercaptopropionyl)-glycine (MPG), which scavenges both peroxynitrite and hydroxyl radicals. For this purpose, isolated rat hearts were subjected to 30 min of global ischemia followed by 30 min of reperfusion, and cardiomyocytes were prepared to monitor changes in the intracellular concentration of free Ca(2+) ([Ca(2+)](i)). Marked depression in the left ventricular developed pressure and elevation in the left ventricular end-diastolic pressure in I/R hearts were attenuated by treatment with NAC or MPG. Cardiomyocytes obtained from I/R hearts showed an increase in the basal level of [Ca(2+)](i) as well as augmentation of the low Na(+)-induced increase in [Ca(2+)](i), with no change in the KCl-induced increase in [Ca(2+)](i). These I/R-induced alterations in Ca(2+) handling by cardiomyocytes were attenuated by treatment of hearts with NAC or MPG. Furthermore, reduction in the isoproterenol-, ATP-, ouabain-, and caffeine-induced increases in [Ca(2+)](i) in cardiomyocytes from I/R hearts were limited by treatment with NAC or MPG. The increases in the basal [Ca(2+)](i), unlike the KCl-induced increase in [Ca(2+)](i), were fully or partially prevented by both NAC and MPG upon exposing cardiomyocytes to hypoxia-reoxygenation, H(2)O(2), or a mixture of xanthine and xanthine oxidase. These results suggest that improvement in cardiac function of I/R hearts treated with NAC or MPG was associated with attenuation of changes in Ca(2+) handling by cardiomyocytes, and the results support the view that oxidative stress due to oxyradical generation and peroxynitrite formation plays an important role in the development of intracellular Ca(2+) overload in cardiomyocytes as a consequence of I/R injury.

  11. Effect of cassava-starch coatings with ascorbic acidic and N-acetylcysteine on the quality of harton plantain (Musa paradisiaca

    Directory of Open Access Journals (Sweden)

    Carlos Julio Márquez Cardozo


    Full Text Available The effect of coatings was evaluated on the quality ofharton plantain fruits (Musa paradisiaca in the postharvest stage.Solutions for three treatments were prepared from 50 g L-1 cassavastarch (Manihot esculenta with 30 g L-1 glycerol as the plasticizerand 6 g L-1 polyethylene glycol-600®; for anti-browning agents, 6g L-1 ascorbic acid (AA and 8 g L-1 N-Acetyl-Cysteine (NAC wereadded. The fruits were coated by immersion, stored at 18 ± 4°Cand 85% RH. Physicochemical properties were determined at 32days postharvest. The applied coatings decreased the physiologicalweight loss (%WL and resulted in a higher pulp firmness (PF; nosignificant difference was seen with a confidence level of 95% inthe concentration of total soluble solids (TSS, acidity or maturityindex. The skin color was measured by the CIE L*a*b* method, withan average L value of 70 for the fruits coated with the 6 g L-1 AAand 8 g L-1 NAC mixture, while the control fruits had a value of 57.Also, lower a* coordinate values and browning indices were foundfor the epidermis of the coated fruits. The enzymatic activity of thepolyphenol oxidase decreased with the number of postharvest daysfor all of the treatments, being lower for the fruits with the mixtureof anti-browning agents by 27%, as compared to the control. It wasconcluded that the coating mixture containing the anti-browningagents ascorbic acid, 6 g L-1, and N-acetyl-cysteine, 8 g L-1, showeda better effect as an alternative for storing fruits and prolongingthe shelf-life of harton plantain.

  12. Recovery by N-acetylcysteine from subchronic exposure to Imidacloprid-induced hypothalamic-pituitary-adrenal (HPA) axis tissues injury in male rats. (United States)

    Annabi, Alya; Dhouib, Ines Bini; Lamine, Aicha Jrad; El Golli, Nargès; Gharbi, Najoua; El Fazâa, Saloua; Lasram, Mohamed Montassar


    Imidacloprid is the most important example of the neonicotinoid insecticides known to target the nicotinic acetylcholine receptor in insects, and potentially in mammals. N-Acetyl-l-cysteine (NAC) has been shown to possess curative effects in experimental and clinical investigations. The present study was designed to evaluate the recovery effect of NAC against Imidacloprid-induced oxidative stress and cholinergic transmission alteration in hypothalamic-pituitary-adrenal (HPA) axis of male rats following subchronic exposure. About 40 mg/kg of Imidacloprid was administered daily by intragastric intubation and 28 days later, the rats were sacrificed and HPA axis tissues were removed for different analyses. Imidacloprid increased adrenal relative weight and cholesterol level indicating an adaptive stage of the general alarm reaction to stress. Moreover, Imidacloprid caused a significant increase in malondialdehyde level, the antioxidants catalase, superoxide dismutase and glutathione-S-transferase showed various alterations following administration and significant depleted thiols content was only recorded in hypothalamic tissue. Furthermore, the hypothalamic and pituitary acetylcholinesterase activity and calcium level were significantly increased highlighting the alteration of cholinergic activity. The present findings revealed that HPA axis is a sensitive target to Imidacloprid (IMI). Interestingly, the use of NAC for only 7 days post-exposure to IMI showed a partial therapeutic effect against Imidacloprid toxicity.

  13. Transport mechanism of chitosan-N-acetylcysteine, chitosan oligosaccharides or carboxymethyl chitosan decorated coumarin-6 loaded nanostructured lipid carriers across the rabbit ocular. (United States)

    Li, Jinyu; Tan, Guoxin; Cheng, Bingchao; Liu, Dandan; Pan, Weisan


    To facilitate the hydrophobic drugs modeled by coumarin-6 (Cou-6) acrossing the cornea to the anterior chamber of the rabbit eye, chitosan (CS) derivatives including chitosan-N-acetyl-l-cysteine (CS-NAC), chitosan oligosaccharides (COS) and carboxymethyl chitosan (CMCS) modified nanostructured lipid carriers (NLCs) were designed and characterized. We found that, with similar size distribution and positivecharges, different CS derivatives based on NLCs led to distinctive delivery performance. In vivo precorneal retention study on rabbits revealed that these CS derivatives coating exhibited a stronger resistant effect than Cou-6 eye drops and Cou-6-NLC (P<0.05), moreover, the AUC (0-∞) , C max and MRT (0-∞) of them followed the sequence of CMCS-Cou-6-NLC

  14. The efficacy of N-acetylcysteine to protect the human cochlea from subclinical hearing loss caused by impulse noise: A controlled trial

    Directory of Open Access Journals (Sweden)

    Ann-Cathrine Lindblad


    Full Text Available In military outdoor shooting training, with safety measures enforced, the risk of a permanent, noise-induced hearing loss is very small. But urban warfare training performed indoors, with reflections from walls, might increase the risk. A question is whether antioxidants can reduce the negative effects of noise on human hearing as it does on research animals. Hearing tests were performed on a control group of 23 military officers before and after a shooting session in a bunker-like room. The experiments were repeated on another group of 11 officers with peroral adminstration of N-acetyl-cysteine (NAC, directly after the shooting. The measurements performed were tone thresholds; transient-evoked otoacoustic emissions, with and without contralateral noise; and psycho-acoustical modulation transfer function (PMTF, thresholds for brief tones in modulated noise. Effects from shooting on hearing thresholds were small, but threshold behavior supports use of NAC treatment. On the PMTF, shooting without NAC gave strong effects. Those effects were like those from continuous noise, which means that strict safety measures should be enforced. The most striking finding was that the non-linearity of the cochlea, that was strongly reduced in the group without NAC, as manifested by the PMTF-results, was practically unchanged in the NAC-group throughout the study. NAC treatment directly after shooting in a bunkerlike room seems to give some protection of the cochlea.

  15. Development and application of dynamic MR-imaging for evaluation of perfusion changes in rectal carcinoma during a course of radiotherapy in clinical use. Preliminary results; Entwicklung und Anwendung dynamischer MRT-Messungen zur Evaluierung von Perfusionsveraenderungen bei Rektumkarzinomen unter Bestrahlung in der klinischen Routine. Erste Ergebnisse

    Energy Technology Data Exchange (ETDEWEB)

    Vries, A. de; Griebel, J.; Gneiting, T.; Hoflehner, J.; Brandl, M.; Lukas, P. [Leopold-Franzens-Univ. Innsbruck (Austria). Universitaetsklinik fuer Strahlentherapie - Radioonkologie; Judmaier, W.; Kremser, C.; Schocke, M.; Aichner, F. [Leopold-Franzens-Univ. Innsbruck (Austria). Inst. fuer Magnetresonanztomographie; Peer, S.; Rettl, G. [Leopold-Franzens-Univ. Innsbruck (Austria). Universitaetsklinik fuer Radiodiagnostik I; Oefner, D. [Leopold-Franzens-Univ. Innsbruck (Austria). Universitaetsklinik fuer Chirurgie; Debbage, P. [Leopold-Franzens-Univ. Innsbruck (Austria). Inst. fuer Histologie und Embryologie


    procedures. (orig.) [German] Ziel: Entwicklung und Anwendung dynamischer Magnetresonanztomographiemessungen zur Erhebung von Perfusionsparametern bei Rektumkarzinomen unter Bestrahlung in der klinischen Routine. Patienten und Methode: Bei Rektumkarzinompatienten (n=8), die sich einer praeoperativen kombinierten Radiochemotherapie unterzogen, wurden Perfusionsdaten erhoben. An einem 1,5-Tesla-Ganzkoerperkernspintomographen wurden ultraschnelle T1-Mapping-Sequenzen zum Erhalt von T1-Maps mit Intervallen von 14 und 120 Sekunden implementiert. Die Messzeit der dynamischen Messungen betrug 40 Minuten. Die Messschicht (Schichtdicke 5 mm) wurde so gewaehlt, dass sowohl Tumor als auch arterielle Gefaesse dargestellt wurden. Gadolinium-DTPA-(Gd-DTPA-)Konzentrations-Zeit-Kurven wurden nach einem prolongierten Bolus im arteriellen Blut und im Tumor berechnet. Die angewendete Methode erlaubte eine raeumliche Aufloesung von 2x2x5 mm und eine zeitliche Aufloesung von 14 Sekunden. Die Messdaten wurden vor und in konstanten Intervallen waehrend Therapie erhoben. Ergebnisse: Die raeumliche und zeitliche Aufloesung der T1-Maps war ausreichend, um Areale mit unterschiedlicher Kontrastmittelkinetik innerhalb des Tumors zu erfassen sowie die grossen Beckenarterien sicher zu identifizieren. Bei sechs Patienten konnten Gd-DTPA-Konzentrationskurven im Tumor unter Therapie erhoben werden. Der Perfusionsindex (Pi) versus Strahlendosis zeigte eine signifikante Zunahme in der ersten oder zweiten Woche der Bestrahlung, bevor er entweder kontinuierlich absank oder nach anfaenglichem Abfall einen erneuten Anstieg aufwies. Der durchschnittliche Pi-Ausgangswert betrug 0,16 ({+-}0,049), das durchschnittliche Pi-Maximum war 0,23 ({+-}0,058). Die relativen Perfusionsveraenderungen betrugen zwischen 20 und 83%. Schlussfolgerung: Unsere Ergebnisse zeigen, dass sich die verwendete Methode zur Erfassung von Perfusionsparametern unter Bestrahlung eignet und in der klinischen Routine anwendbar ist. In der Zukunft

  16. Development and application of a NaNiCl{sub 2}-battery for industrial trucks regarding the requirements cycle life, safety and reliability. Final report; Entwicklung und Erprobung einer NaNiCl{sub 2}-Batterie fuer Flurfoerderzeuge unter besonderer Beruecksichtigung der Aspekte Lebensdauer, Sicherheit und Zuverlaessigkeit. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Boehm, H.; Beyermann, G.; Bulling, M.


    Two NaNiCl{sub 2}-battery types including the battery controller and peripheral equipment have been developed for the application in fork lift trucks and driverless transportation. The batteries have been tested and evaluated on bench testing as well as in fork lift trucks and driverless trucks in practical application. The field tests have been performed by the subcontractors Still, Mercedes-Benz and Indumat. All test results have shown that the NaNiCl{sub 2}-battery (ZEBRA-battery) is well suited for the application in industrial traction. A series development together with a cost reduction programme have to be performed ahead of the introduction of the ZEBRA-battery into the market for industrial traction batteries. (orig.) [Deutsch] Im Rahmen des Vorhabens wurden zwei NaNiCl{sub 2}-Batterietypen, eine fuer die Anwendung in Gabelstaplern, die andere fuer den Einsatz in fahrerlosen Transportsystemen (FTS), einschliesslich des Batteriesteuergeraetes und der Batterieperipherie entwickelt. Die Batterien wurden auf Teststaenden im Labor sowie in Gabelstaplern und FTS unter Praxisbedingungen erprobt. Der praktische Einsatz erfolgte bei den Unterauftragnehmern Still, Mercedes-Benz und Indumat. Die Ergebnisse haben gezeigt, dass die NaNiCl{sub 2}-Batterie (ZEBRA-Batterie) fuer die Anwendung in Gabelstaplern und FTS geeignet ist. Insbesondere wurde nachgewiesen, dass die NaNiCl{sub 2}-Batterie im Gegensatz zu anderen Batteriesystemen einen Zweischichtbetrieb von Gabelstaplern ohne Batteriewechsel ermoeglicht. Vor Einfuehrung der ZEBRA-Batterie in den Flurfoerdermarkt muss die Serienentwicklung bei gleichzeitiger Senkung der Herstellkosten durchgefuehrt werden. (orig.)

  17. Power to gas to relieve the electricity distribution networks. Continuation of the study ''Benefit of smart grid concepts taking into account the PtG technology''; Power-to-Gas zur Entlastung der Stromverteilungsnetze. Fortsetzung der Studie ''Nutzen von Smart-Grid-Konzepten unter Beruecksichtigung der PtG-Technologie''

    Energy Technology Data Exchange (ETDEWEB)

    Huettenrauch, Jens; Zoellner, Sylvana; Mueller-Syring, Gert [DBI Gastechnologisches Institut gGmbH, Freiberg (Germany); and others


    One of the main challenges in the energy transition is to integrate the increasing based on volatile renewable energy generated power in market and network. Based on actual distribution networks in Emsland in the study presented below was investigated, what contribution an intelligent coupling of electricity and gas distribution networks by means of power to gas (PtG) systems can do to solve this challenge. The investigations have shown, among other things, that the network expansion costs in particular can be significantly reduced by the coupling when otherwise an oversizing would be done by discrete power grid expansion measures. [German] Eine der wesentlichen Herausforderung bei der Energiewende besteht darin, die zunehmend auf volatilen erneuerbaren Energien basierende Stromerzeugung in Markt und Netz zu integrieren. Anhand realer Verteilungsnetze im Emsland wurde in der im Folgenden vorgestellten Studie untersucht, welchen Beitrag eine intelligente Kopplung von Strom- und Gasverteilungsnetzen mittels Power-to-Gas (PtG)-Anlagen zur Loesung dieser Herausforderung leisten kann. Die Untersuchungen haben unter anderem gezeigt, dass durch die Kopplung die Netzausbaukosten insbesondere dann signifikant reduziert werden koennen, wenn andernfalls eine Ueberdimensionierung durch diskrete Stromnetzausbaumassnahmen erfolgen wuerde.

  18. {sup 31}P-MR-spectroscopy of the skeletal muscles under load: demonstration of normal energy metabolism compared to different neuromuscular diseases; {sup 31}P-MR-Spektroskopie der peripheren Skelettmuskulatur unter Belastung: Darstellung des normalen Energiestoffwechsels im Vergleich zu metabolischen Muskelerkrankungen

    Energy Technology Data Exchange (ETDEWEB)

    Block, W.; Traeber, F.; Kuhl, C.K.; Keller, E.; Rink, H.; Schild, H.H. [Bonn Univ. (Germany). Radiologische Klinik; Lamerichs, R. [Philips Medical Systems, Best (Netherlands); Karitzky, J. [Bonn Univ. (Germany). Neurologische Klinik


    Purpose: {sup 31}P-MR spectroscopy of skeletal muscle under ecercise was used to obtain the range of normal variation and comparison was made for different neuromascular diseases. Methods: 41 examinations of 24 volunteers and 41 investigations in 35 patients were performed on 1.5 T MR systems (Gyroscan S15 und S15/ACSII, Philips). Localised {sup 31}P-MR spectra of the calf muscle were obtained in time series with a resolution of 12 s. Results: Two types of muscle energy metabolism were identified from the pattern of spectroscopic time course in volunteers: While the first group was characterised by a remarkable decline to lower pH values during exercise, the second group showed only small pH shifts (minimum pH: 6.48{+-}0.13 vs 6.87{+-}0.07, p<10{sup -6}) although comparable workload conditions were maintained. The pH-values correlated well with blood lactate analysis. Patients with metabolic disorders and chronic fatigue syndrome (CFS) showed decreased resting values of PCr/(PCr+P{sub i}) and increased pH levels during exercise. PCr recovery was significantly delayed (0.31 vs 0.65 min{sup -1}, p<0.00005) in metabolic muscle disorders but was normal in CFS patients. Conclusion: Findings in volunteers indicate utilisation of different metabolic pathways which seems to be related to the fibre type composition of muscle. Reduced resting levels for PCr/(PCr+P{sub i}), altered pH time courses, and decreased PCr recovery seem to be helpful indicators for diagnosis of metabolic muscle disorders. (orig./MG) [Deutsch] Ziel: Bestimmung der Bandbreite des gesunden Energiestoffwechsels der peripheren Skelettmuskulatur mit Hilfe der {sup 31}P-MR-Spektroskopie als Grundlage der Diagnostik neuromuskulaerer Erkrankungen. Methoden: In Mehrfachuntersuchungen an 24 Probanden und 35 Patienten in 1,5 T MR-Systemen (Gyroscan S15 und S15/ACSII, Philips) wurden {sup 31}P-MR-Spektren unter Belastung akquiriert. Ergebnisse: Die Probanden liessen sich streng in zwei Gruppen einteilen

  19. MC-PELMO 3.0 - a computer model to estimate groundwater contamination caused by leaching of wood preservatives from storage sites of treated wood in Germany; Grundwassergefaehrdung durch Holzschutzmittel. MCPELMO 3.0 - ein mathematische Simulationsprogramm zur Abschaetzung der Grundwassergefaehrdung unter Holzlagerflaechen in Deutschland

    Energy Technology Data Exchange (ETDEWEB)

    Klein, M. [Fraunhofer-Inst. fuer Molekularbiologie und Angewandte Oekologie, Schmallenberg-Grafschaft (Germany); Herrmann, M. [Umweltbundesamt, Berlin (Germany)


    Methods. Based on already in-use versions of the leaching model PELMO, an advanced version (MC-PELMO 3.0) was developed with a specific focus on estimating groundwater contamination under storage sites at wood preserving facilities in Germany. The model processes twenty-two different leaching scenarios that were derived from twelve characteristic soil profiles representing pedological regions in Germany along with recorded meteorological data from nine weather stations. These data are related to geographic distribution of industrial wood preserving activity. The model calculates statistic probabilities of concentrations of wood preservatives in seepage water beneath timber storage sites of regions to be selected by the user. Results and Discussion. The reports provided by MC-PELMO 3.0 include mean average concentrations, 55 to 99 percentiles, and single maximum concentrations for each of the scenarios. The results can be related to the total area of Germany, its forest area or to the density of preservation activity in various regions. Beside concentrations of the parent compounds, those of degradation products may be calculated for the seepage water. Conclusion. The described model is a particularly useful tool for comparative assertion of various wood preservative products under aspects of the exposure of groundwater resources. Comparative assertion is a new element within EU-chemicals policy, for the first time materialized in the biocidal products directive 98/8/EC. Furthermore, the results of model calculations identify vulnerable regions in Germany for which appropriate risk management measures have to be taken in order to protect groundwater from contamination. (orig.) [German] Methoden. Basierend auf bestehenden Versionen des Versickerungsmodells PELMO wurde eine Version speziell fuer die Abschaetzung des Versickerungsverhaltens von Holzschutzmitteln unter Lagerplaetzen von Holz-Impraegnierbetrieben in Deutschland entwickelt. Das stochastische Modell

  20. Distribution of heavy metals in a wood culture water catchment area under the influence of acid deposition as shown by the example of the Soese trough (Western Harz region); Verteilung von Schwermetallen in einem forstlich genutzten Wassereinzugsgebiet unter dem Einfluss saurer Deposition am Beispiel der Soesemulde (Westharz)

    Energy Technology Data Exchange (ETDEWEB)

    Andreae, H. [ed.


    This thesis deals with the distribution and turnover of heavy metals in partial terrestrial ecosystems of the water catchment area of the Soese storage dam in the Western Harz region, a forested area affected by acid deposition. The metals investigated are cadmium, chromium, cobalt, copper, nickel, lead and zinc. The work distinguishes three spatial areas: Regional heavy metal pollution is characterized via the establishment of nine deposition measuring sites (recent pollutant stress) in the field or under spruce stands and via cadastre-oriented surveying of element contents and accumulations in humus ground covers and mineral soils (historical pollutant stress). In-soil heavy metal turnovers are documented in five representative sites via investigation of the soil solution (years of measurement 1989-1991). In three sites, heavy metal turnovers are quantified via flow balances for the terrestrial ecosystem and the soil zone free of roots. (orig.) [Deutsch] Die vorliegende Arbeit befasst sich mit der Verteilung und dem Umsatz von Schwermetallen in terrestrischen Teiloekosystemen des bewaldeten, durch saure Deposition beeinflussten Wassereinzugsgebiets der Soese-Talsperre im Westharz. Untersucht werden die Metalle Cadmium, Chrom, Kobalt, Kupfer, Nickel, Blei und Zink. In der Arbeit werden drei raeumliche Ebenen unterschieden: Die regionale Belastung wird ueber die Einrichtung von neun Depositionsmessstellen [rezente Belastung] im Freiland bzw. unter Fichtenbestaenden sowie ueber eine katasterorientierte Erfassung von Element-Gehalten und -Vorraeten in Humusauflagen und Mineralboeden [historische Belastung] charakterisiert. Die bodeninternen Umsaetze der Schwermetalle werden an fuenf repraesentativen Standorten anhand der Untersuchung der Bodenloesung dokumentiert (Messjahre 1989-1991). An drei Standorten werden die Schwermetall-Umsaetze ueber Flussbilanzen fuer das terrestrische Oekosystem und die undurchwurzelte Bodenzone quantifiziert. (orig.)

  1. Solar reactors for freshwater treatment in developing countries with high radiation intensity, with particular regard to part-project No. 1: Microbiology, process conception. Final report; Solarreaktoren fuer die Trinkwasseraufbereitung in Entwicklungslaendern mit hoher Sonnenstrahlungsintensitaet unter besonderer Beruecksichtigung von Teilprojekt 1: Mikrobiologie, Verfahrenskonzeption. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Watzke, E.; Roth, M.; Hoffmeier, C.


    Erhoehung der Bakterienabtoetungsraten wird die erhoehte Temperatur des behandelten Wassers in einem isolierten Sammelbehaelter ueber mehrere Stunden gehalten. Ausserdem wurden Trinkwasserentkeimungsgefaesse (TWEG) aus hoch UV-durchlaessigem Glas entwickelt, die ebenfalls zur Trinkwasserdesinfektion verwendet werden koennen. In einem Folgeprojekt werden mit den TWEA Langzeittests unter praxisrelevanten Anwendungsbedingungen in Entwicklungslaendern durchgefuehrt. (orig.)

  2. Development of an analysis method for determining chlorinated hydrocarbons in marine sediments and suspended matter giving particular consideration to supercritical fluid extraction; Entwicklung eines Analysenverfahrens zur Bestimmung von chlorierten Kohlenwasserstoffen in marinen Sedimenten und Schwebstoffen unter besonderer Beruecksichtigung der ueberkritischen Fluidextraktion

    Energy Technology Data Exchange (ETDEWEB)

    Sterzenbach, D.


    diesem Grund soll das gesamte Verfahren und dessen Einzelschritte unter diesem Gesichtspunkt ueberprueft und gegebenenfalls verbessert werden. Fuer die Probennahme von marinen Schwebstoffen soll ein Verfahren erarbeitet werden, das eine effiziente Abtrennung moeglichst kleiner suspendierter Partikel aus Seewasser ermoeglicht. Mit dem entwickelten Analysenverfahren sollen aktuelle Aspekte der Meereschemie zu Quellen, Transport, Verteilung und Verbleib von chlorierten Kohlenwasserstoffen in der marinen Umwelt behandelt werden. (orig.)

  3. In situ measurement of flow characteristics of natural saline rock in loose zones for gas and saline solutions in given rock stress conditions. Final report; In-situ-Ermittlung von Stroemungskennwerten natuerlicher Salzgesteine in Auflockerungszonen gegenueber Gas und Salzloesungen unter den gegebenen Spannungsbedingungen im Gebirge. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Haefner, F.; Belohlavek, K.U.; Behr, A.; Foerster, S.; Pohl, A.


    A method and equipment were developed for measuring the extension of loose zones around worked areas in saline rock and for in situ measurement of very small permeabilities and porosities in these zones. The experiments are based on unsteady borehole logs with flowing gases or liquids with special multiple pack systems that enable measurements from 4 cm to 15 m from the cavern contour. The measurements were evaluated by a specially developed software with automatic parameter identification. Permeabilities were identified between 10{sup 14} m{sup 2} and the detection limit of 10{sup 24} m{sup 2} and effective porosities of less than 0.1% at experimental times of several minutes up to several days. The logs were made in 3 mines in Stassfurt rock salt at depths of 700 and 500 m with different geological and geomechanical boundary conditions, worked in different ways and for different periods of time (between a few days and 37 years). Some of the findings were validated by ultrasonic measurements. [German] Fuer die Ermittlung der Ausdehnung von Auflockerungszonen um bergmaennisch aufgefahrene Strecken/Hohlraeume im Salzgestein und zur In-situ-Bestimmung kleinster Permeabilitaeten und Porositaeten in diesen Bereichen wurde ein Verfahren und eine praktikable Versuchsausruestung entwickelt. Diese eignet sich auch fuer Frac-Untersuchungen. Basis der Versuchsdurchfuehrungen sind instationaere Bohrlochuntersuchungen mit Gasen oder Fluessigkeiten als Stroemungsfluid unter Einsatz spezieller Mehrfachpackersysteme. Damit sind Messungen ab 4 cm Abstand zur Hohlraumkontur bis zu 15 m moeglich. Die Versuchsauswertung erfolgt mittels einer speziell entwickelten Software mit automatischer Parameteridentifikation, die die instationaere Stroemung um die Versuchsbohrung raeumlich vollstaendig beschreibt. Permeabilitaeten wurden je nach Abstand zur Hohlraumkontur zwischen 10{sup -14} m{sup 2} und der Nachweisgrenze 10{sup -24} m{sup 2} und effektive Porositaeten bis <0,1% ermittelt, bei

  4. Stable and flexible under power; Stabil und flexibel unter Strom

    Energy Technology Data Exchange (ETDEWEB)

    Irrgang, Reinhard


    If conveyor routes, shelve service devices, hall cranes or other haulage equipment - all this systems want to be supported economically, securely and reliably by energy. Optimized energy chains and gliding connect with innovative details function as probate systems. Also touchless energy- and data transmission technologies open an immense potential. (orig./GL)

  5. Vehicles under electricity. Result booklet; Autos unter Strom. Ergebnisbroschuere

    Energy Technology Data Exchange (ETDEWEB)



    The booklet under consideration reports on the environmental relief of electric-driven vehicles by means of the research project OPTUM 'Potentials of environmental relief of electric driven vehicles - Integrated analysis of vehicle usage and energy economy'. Experts from the Institute for Applied Ecology (Freiburg, Federal Republic of Germany) and the Institute for Social-Ecological Research (Frankfurt (Main), Federal Republic of Germany) are devoted to the following issues: (1) What is the acceptance for electric-driven vehicles?; (2) What is their future market potential?; (3) What are the advantages of electromobility with respect to the climate?; (4) Are their supply shortages concerning major raw materials?.

  6. Coalification reactions under pressure. Inkohl-ungsreaktionen unter Druck

    Energy Technology Data Exchange (ETDEWEB)

    Huck, G.; Patteisky, K.


    Studies of the influence of pressure on the coalification reactions in hard coal (temperature ranges from 240 to 350/sup 0/C and pressures ranging from 0.1 to 8000 ata) have shown that coalification reactions in younger coals are impeded by higher mechanical pressures. The relationship of the mutual effect of pressure and temperature on the coalification reactions may be illustrated by graphs, if the parameters are determined, which cause an increase in the reflection index of the vitrinite of 0.89 to 1.0 and liberate 8 liters of gas per kilogram of finely ground coal, at 0.5 mm Hg. The results indicate, contrary to theories often expressed in the literature, that the intensifying influence of pressure on coalification may be excluded if the pressure charge operates through shear- and friction-forces, without temperature changes. The results also show that the use of spores as coalification thermometers is limited. It is shown that at higher pressures spores may be exposed to temperatures above 200/sup 0/C without showing thermal modifications.

  7. Der antiskeptische Boden unter dem Gehirn im Tank


    Müller, Olaf L.


    Crispin Wright hat die bislang beste Rekonstruktion von Putnams Beweis gegen die skeptische Hypothese vom Gehirn im Tank vorgelegt. Aber selbst in Wrights Fassung hat der Beweis einen Mangel: Er wird mithilfe eines Prädikates wie z.B. "Tiger" geführt und funktioniert nur, wenn man sich darauf verlassen kann, dass es Tiger wirklich gibt. Aber die Skeptikerin bestreitet, über die Existenz von Tigern bescheid zu wissen. Das Problem lässt sich dadurch beheben, dass man den Beweis – statt mit dem ...

  8. Electricity trade under financial market supervision; Der Stromhandel unter Finanzmarktaufsicht

    Energy Technology Data Exchange (ETDEWEB)

    Hagena, Martin


    With the competitive opening of the electricity market at European and national level, the goods electricity became a freely traded commodity. The author of the contribution under consideration describes the legal consequences related to financial market for trading electricity in the context of the current Directive 2004/39/EC now under consideration of the commodity futures trading in its representational scope. The statements clearly indicate that the power market is a goods market with its own laws and not a classical financial market. It considers what characteristics exist in electricity trading and whether and how they are considered for regulatory purposes.

  9. Mythos Qualifikation – Berufungsverfahren unter der gleichstellungspolitischen Lupe

    Directory of Open Access Journals (Sweden)

    Eva Blome


    Full Text Available Auf der Grundlage von qualitativen Interviews und einer Vollerhebung aller Verfahrensregelungen liefert die Studie eine umfassende Analyse der Gestaltung von Berufungsverfahren aus gleichstellungspolitischer Perspektive. Aufgrund der ergänzenden konkreten Handlungsempfehlungen zum gleichstellungsorientierten Verfahrensmanagement verhilft die Untersuchung nicht nur zu einer empirisch fundierten Einsicht in die Diskriminierungsmechanismen an deutschen Hochschulen, sondern stellt zudem einen wichtigen Beitrag für die Integration von Gleichstellung in die aktuellen Reformen von Berufungsverfahren dar.

  10. Akustische und perzeptive Analysen von Sprache unter Alkoholeinfluss


    Baumeister, Barbara Susanne


    In this thesis, several acoustic and perceptive studies on the influence of alcoholic intoxication on speech are presented. The speech samples for the experiments are taken from the Alcohol Language Corpus, a large database containing recordings of 162 (77 female and 85 male) speakers in both sober and intoxicated conditions with various blood alcohol concentrations. An acoustic analysis of recordings of 148 speakers in intoxicated and sober condition reveals a significant increase of the mea...

  11. Development of an ultratrace analysis method for elements of the platinum group contained in environmental and geological samples using an ICP-QMS with isotope dilution analysis and suitable separation techniques; Entwicklung eines Verfahrens zur Ultraspurenbestimmung der Platingruppenelemente in Umwelt- und geologischen Proben mit einem ICP-QMS unter Verwendung der Isotopenverduennungsanalyse sowie geeigneter Separationstechniken

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, M.


    environment of elements of the platinum group. [German] Schwerpunkt dieser Arbeit war die Verfahrensentwicklung zur Ultraspurenbestimmung der PGE in Umwelt- und geologischen Proben unter Verwendung der massenspektrometrischen Isotopenverduennungsanalyse mit anschliessender Bestimmung an einem Quadrupol ICP-MS (ICP-QMSIVA). Das in dieser Arbeit entwickelte Verfahren zur Ultraspurenbestimmung der PGE mit ICPQMSIVA wurde im Rahmen des Projektes ''Production and certification of a road dust reference material for platinum, palladium and rhodium (PGEs) in automative catalytic converters (PACEPAC)'' der Europaeischen Union zur Zertifizierung von zwei Referenzmaterialien fuer Umweltproben eingesetzt. Hierbei wurde in der Vorrunde der Zertifizierung (''intercomparison round'') eine sehr gute Uebereinstimmung der Ergebnisse mit dem gewichteten Mittelwert der Ergebnisse fuer Pd und Pt mit den uebrigen teilnehmenden Laboratorien festgestellt. Die mit der hier entwickelten Methode erhaltenen Ergebnisse wurden ohne Ausnahme fuer alle gemessenen Elemente zur vorlaeufigen Zertifizierung herangezogen. Damit leistete die vorliegende Arbeit einen erheblichen Beitrag zum erfolgreichen Abschluss dieses Projekts. Den Erwartungen hinsichtlich Richtigkeit und Reproduzierbarkeit des entwickelten Verfahrens wurde somit voll entsprochen. Erneut konnte die grosse Bedeutung der Isotopenverduennungstechnik fuer die Zertifizierung von Referenzmaterialien aufgezeigt werden, da mit dieser Technik bei sachgerechtem Einsatz Ergebnisse hoher Richtigkeit erzielt werden. Durch vergleichende Messungen mit der NiS-Dokimasie und NAA, die in einer Kooperation mit dem Kernchemischen Institut der Universitaet Mainz durchgefuehrt wurden, und dem hier verwendeten Verfahren, konnten uebereinstimmende Daten, hinsichtlich der Abnahme der Konzentrationen von Pd und Pt in Abhaengigkeit von der Entfernung zu einer Autobahn, erzielt werden. Diese Arbeit und die Forschungsergebnisse, die

  12. Investigation and modelling of the alkaline release and transport during coal combustion at elevated pressures. Final report; Untersuchung und Modellierung der Freisetzungs- und Transportvorgaenge von Alkalien bei der Kohleverbrennung unter hohen Druecken. Untersuchungen mit der Hochdruck-Hochtemperatur-Thermowaage, Alkalienanalysen in Rohkohlen und Feuerungsversuche in der Druckwirbelschichtanlage FRED (DMT). Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Bonn, B.; Steffin, C.; Busch, U.; Mayer, M.


    Verbrennung unter Druck. Variiert wurden hierzu die Einflussparameter umgebende Gasatmosphaere und Aschezusammensetzung sowie Druck und Aufheizrate. Die Versuche wurden in der DMT Hochdruck-Hochtemperatur-Thermowaage (TGA) und der DMT Druckwirbelschichtfeuerung (DWSF) durchgefuehrt. TGA-Versuche: Als Modelsubstanz fuer Alkalien wurde Natriumchlorid und fuer die Mineralsubstanz der Kohle Metakaolin eingesetzt. Die chemische Eigenschaft (Basizitaet) von Metakaolin wurde durch Zugabe von CaO veraendert. Die allgemein akzeptierte Annahme eines physikalischen Verdampfungsprozesses der Alkalien aus den Kohleaschen konnte in den TGA-Versuchen nicht bestaetigt werden. Vielmehr muss von einer Desorptionsreaktion des Natriumchlorids von der Metakaolin-Oberflaeche ausgegangen werden. Die Desorption von Alkalien wird nicht durch die chemische Zusammensetzung der Mineralsubstanz beeinflusst, dagegen besitzen die Sauerstoffverbindungen in der Gasphase und der Druck einen starken Einfluss. DWSF-Versuche: Die Versuchsanlage wurde betrieben bei einer Wirbelschichttemperatur von 920 C und einem Druck von 7 bar. Die online/in-situ Alkali-Messung beruht auf der Excimer Laser Induced Fragmentation Fluorescence ELIF. Die Alkali-Konzentration wurde variiert durch die Zugabe von Natriumacetat und Natriumchlorid. Als Adsorber von Alkaliemissionen wurde Kaolin eingesetzt. Darueber hinaus wurde der Einfluss der Kalksteinzugabe auf die Alkali-Emissionen untersucht. Die Versuche zeigten u.a., dass die Alkali-Emissionen von Braunkohlen um einen Faktor 50-100 ueber den Alkali-Emissionen von Steinkohlen liegen, und dass die Zugabe von Kalkstein (der zur SO{sub 2}-Minderung eingesetzt wird) zu stark ansteigenden Alkalichlorid-Emissionen fuehrt. (orig.)

  13. Effect of N-acetylcysteine on the accuracy of the prothrombin time assay of plasma coagulation factor II+VII+X activity in subjects infused with the drug. Influence of time and temperature

    DEFF Research Database (Denmark)

    Thorsen, Sixtus; Teisner, Ane; Jensen, Søren Astrup


    OBJECTIVES: The prothrombin time (PT) assay of factor II+VII+X activity is an important predictor of liver damage in paracetamol poisoned patients. It complicates interpretation of results that the antidote, acetylcysteine (NAC) depresses this activity. The aim was to investigate if NAC influences...... to plasma in vitro decreased factor II+VII+X activity at 37 degrees C in a time-dependent manner. This effect was quenched at temperatures ... to a significant additional depression of factor II+VII+X activity in plasma from subjects infused with NAC during the first 3h of infusion indicating that it contained reactive NAC. The risk that this NAC interfered with the accuracy of the PT assay was considered minimal with samples stored below 24 degrees C...

  14. Effect of N-acetylcysteine on the accuracy of the prothrombin time assay of plasma coagulation factor II plus VII plus X activity in subjects infused with the drug. Influence of time and temperature

    DEFF Research Database (Denmark)

    Thorsen, S.; Teisner, A.; Jensen, S.A.


    Objectives: The prothrombin time (PT) assay of factor II+VII+X activity is an important predictor of liver damage in paracetamol poisoned patients. It complicates interpretation of results that the antidote, acetylcysteine (NAC) depresses this activity. The aim was to investigate if NAC influences...... added to plasma in vitro decreased factor II+VII+X activity at 37 degrees C in a time-dependent manner. This effect was quenched at temperatures 24 degrees C. Activity lost at 37 degrees C could partly be recovered by subsequent incubation at 5 or 20 degrees C. Incubation at 37 degrees C prior to assay...... led to a significant additional depression of factor II+VII+X activity in plasma from subjects infused with NAC during the first 3h of infusion indicating that it contained reactive NAC. The risk that this NAC interfered with the accuracy of the PT assay was considered minimal with samples stored...

  15. Effect of N-acetylcysteine on the accuracy of the prothrombin time assay of plasma coagulation factor II+VII+X activity in subjects infused with the drug. Influence of time and temperature. (United States)

    Thorsen, Sixtus; Teisner, Ane; Jensen, Søren Astrup; Philips, Malou; Dalhoff, Kim; Bendtsen, Flemming


    The prothrombin time (PT) assay of factor II+VII+X activity is an important predictor of liver damage in paracetamol poisoned patients. It complicates interpretation of results that the antidote, acetylcysteine (NAC) depresses this activity. The aim was to investigate if NAC influences the accuracy of the plasma PT assay. The accuracy of Nycotest PT was studied using plasma added NAC in vitro and plasma from subjects infused with NAC. The latter results were compared with those obtained by analysis of PT by CoaguChek S. Therapeutic NAC concentrations added to plasma in vitro decreased factor II+VII+X activity at 37 degrees C in a time-dependent manner. This effect was quenched at temperatures depression of factor II+VII+X activity in plasma from subjects infused with NAC during the first 3h of infusion indicating that it contained reactive NAC. The risk that this NAC interfered with the accuracy of the PT assay was considered minimal with samples stored below 24 degrees C. This was supported by similarity of results obtained by analysis of appropriately stored plasma and simultaneously drawn blood by CoaguChek S. Residual reactive NAC does not interfere with the accuracy of the PT assay of plasma stored below 24 degrees C, but NAC-induced loss in activity at 37 degrees C may be partly recovered during subsequent storage below 24 degrees C.

  16. The effect of short-term, high-dose oral N-acetylcysteine treatment on oxidative stress markers in cystic fibrosis patients with chronic P-aeruginosa infection - A pilot study

    DEFF Research Database (Denmark)

    Skov, Marianne; Pressler, Tacjana; Lykkesfeldt, Jens


    Background: Patients with cystic fibrosis (CF) and chronic Pseudomonas aeruginosa lung infection have increased oxidative stress as a result of an imbalance between the production of reactive oxygen species caused by inflammation and their inactivation by the impaired antioxidant systems. Supplem...

  17. Digitale Archivierung papierbasierter Krankenakten von Studienpatienten – Eckpunktepapier des KKSN, der GMDS und der TMF unter Mitwirkung des BfArM und der Landesüberwachungsbehörde Nordrhein-Westfalen [Digital archiving of paper-based patient records of clinical trial subjects – a key issues paper of KKSN, GMDS, and TMF in collaboration with the Federal Institute for Drugs and Medical Devices (BfArM and the Health Authority Northrhine-Westphalia

    Directory of Open Access Journals (Sweden)

    Kohl, Christian D.


    Full Text Available [english] The management and maintenance of paper-based patient record archives involve high running costs. Therefore, many health care providers choose to digitize their paper records subsequently destroying the originals. By now, this is an established procedure for patient records from routine care.However, at present it is unclear under which circumstances the patient records of patients taking part in clinical trials can be destroyed after digitization. Paper-based original records may only be destroyed if their digital copies are recognized as source documents by both sponsors and authorities. The authors believe that this recognition should be possible, as long as the scanned paper-based records fulfill the requirements for certified copies according to the Note for Guidance (ICH-GCP. This requires that, based on the applied rules, it can be proven at any time that the existing digitization process is clearly regulated, and the resulting quality is regularly controlled and sufficiently high.The objective of this key issues paper is to present the manner in which the existing regulatory guidelines can be implemented to allow the digitized patient records being recognized as source documents.An English version of this article will be published in “Clinical Investigation” (Future Science .[german] Das Verwalten und Vorhalten von Archiven für papierbasierte Patientenakten verursacht hohe laufende Kosten. Viele Gesundheitsversorger sind daher dazu übergegangen, ihre Papierakten in einem Scan-Prozess zu digitalisieren und die Originalakten anschließend zu vernichten. Für die Patientenversorgung hat sich dieses Vorgehen bewährt.Zum gegenwärtigen Zeitpunkt ist jedoch unklar, unter welchen Voraussetzungen Krankenakten von Patienten, die an klinischen Studien teilnehmen, nach dem Digitalisieren vernichtet werden können. Die papierbasierten Originalakten können nur dann vernichtet werden, wenn deren digitale Kopien von Sponsoren und Beh

  18. The energy sector between the two poles of the free market system and the public service obligations. Potential and limitations of state control, with an emphasis on the act for reform of the energy industry law (ENG) and the EU Internal Market Directive; Der Energiesektor zwischen Marktwirtschaft und oeffentlicher Aufgabe. Moeglichkeiten und Grenzen staatlicher Steuerung unter besonderer Beruecksichtigung des Gesetzes zur Neuregelung des Energiewirtschaftsrechts und des Europarechts

    Energy Technology Data Exchange (ETDEWEB)

    Hoelzer, F.


    In Part A of the book it will be brought out that despite differing conceptual starting points and despite state control's way of working, there is no alternative to legal control. In co-operative models and consensual solutions in those states with private sector service providers, which exist in diverse forms in the energy sector, too, the law operates as a framework or regulatory warning, or it has a reserve function. An end to state control is not linked to that because the state remains responsible for the achievement of the goals the notification ans definition of which it undertakes as a public duty. The results found are discussed and confirmed in Part B of the book in connection with the reform of the legal framework of the energy industry (the German act named ENG). In Part C of the book it will be shown to what extent legal constitutional limitations stand in the way of state control in the energy sector. (orig./CB) [German] Im ersten Teil der Arbeit wird staatliche Steuerung durch Recht an Beispielen des Energiesektors untersucht, wobei insbesondere Fragen des Wirkens und der Erscheinungsformen staatlicher Steuerung sowie die Auswirkungen der Veraenderung staatlicher Steuerung auf die Rolle des Staates und auf das Verhaeltnis von Staat und Gesellschaft im Vordergrund stehen. Im zweiten Teil werden unter besonderer Beruecksichtigung der europarechtlichen Regelungen die Moeglichkeiten, rechtliche Grenzen und Maengel staatlicher Steuerung im bisherigen System der Elektrizitaetsversorgung in Deutschland dargestellt, da Reformen, um dem Steuerungsanspruch des Staates gerecht zu werden, diesezu beachten haben. Es wird u.a. untersucht, inwieweit Entstaatlichungsstrategien im Rahmen der Reformen im Energiesektor Anwendung finden. Im dritten Teil werden die verfassungsrechtlichen Restriktionen untersucht. (orig./CB)

  19. Pressure vessel failure at high internal pressure; Untersuchungen zum Versagen des Reaktordruckbehaelters unter hohem Innendruck

    Energy Technology Data Exchange (ETDEWEB)

    Laemmer, H.; Ritter, B.


    A RPV failure due to plastic instability was investigated using the ABAQUS finite element code together with a material model of thermal plasticity for large deformations. Not only rotational symmetric temperature distributions were studied, but also `hot spots`. Calculations show that merely by the depletion of strength of the material - even at internal wall temperatures well below the melting point of the fuel elements of about 2000/2400 C - the critical internal pressure can decrease to values smaller than the operational pressure of 16 Mpa. (orig.)

  20. Converter-controlled belt conveyor systems under the difficult conditions; Umrichtergesteuerte Bandanlagen unter schwierigen Bedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Horz, Michael-Josef [RAG Deutsche Steinkohle, Herne (Germany). Abt. Elektrotechnik unter Tage; Mueller, Uwe [RAG Deutsche Steinkohle, Herne (Germany). Servicebereich Technik- und Logistikdienste


    Panel 572 is located at a depth of 1,200 m in the Girondelle seam at the West mine. The panel has a mean thickness of 2.5 m and is designed as a shearer loader face with a length of 460 m. Coal is conveyed to the shaft by six belts with a total length of about 6 km and a total lifting height of 396 m. The main conveyor belt 1 with an installed power of 3 MW links the panel to a bunker. Conveyor belts 2 to 4 each with 500 kW drive power have operated in a stable manner since the start of working of the panel. Due to in-seam drivage with a selective-cut machine of type AM 85 an area with a marked trough was produced in the coal conveyor road. This area is traversed by belt 5. The planning and control of the conveyor belt in this difficult geological environment impose high demands on drive and control technology. To ensure stable operation under different loads a raft of measures was resolved and implemented. The contribution examines the problem and describes the implemented measures in detail. (orig.)

  1. Changes of plant hormone levels in conifers subjected to immissions. Hormongehaltsaenderungen in Nadelbaeumen unter Immissionsbelastung

    Energy Technology Data Exchange (ETDEWEB)

    Christmann, A.; Frenzel, B. (Hohenheim Univ., Stuttgart (Germany, F.R.). Inst. fuer Botanik)


    Effects caused by a reduction of immissions on the phytohormone balance in needles of conifers (ethylene, measured as ACC and MACC, abscisic acid and indole-3-acetic acid) were investigated at the sites 'Edelmannshof' in the Welzheimer Wald, some thirty kilometers to the east of Stuttgart (open-top chambers) and 'Stoeckerkopf' in the Northern Black Forest (under strong SO{sub 2}-impact until autumm 1987). At the 'Edelmannshof', the consequences of the reduced impact of immissions on the phytohormone balance of young spruce trees cannot be differentiated reliably from individual differences between the trees investigated, due to the fact that there the phytohormones mentioned were investigated during one year only. At the site 'Stoeckerkopf' the results point to a different behaviour of IAA-contents in needles of trees formerly subjected to SO{sub 2}-immissions and trees subjected to influences causing forest decline. This corroborates former results of AbA investigations. A method for determining of IAA-contents in needles from fir (Abies alba Mill.) and spruce (Picea abies Karst.) is presented. (orig.) With 13 figs., 22 refs.

  2. Changes of plant hormone levels in conifers subjected to immissions. Hormongehaltsaenderungen in Nadelbaeumen unter Immissionsbelastung

    Energy Technology Data Exchange (ETDEWEB)

    Frenzel, B.; Christmann, A. (Hohenheim Univ., Stuttgart (Germany, F.R.). Inst. fuer Botanik)


    The investigation of effects evoked by reduction of immissions on the phytohormone balance in needles of conifers (ethylene, measured as ACC and MACC, abscisic acid and indole-3-acetic acid) is continued on the sites 'Edelmannshof' in the Welzheimer Wald (open-top chambers) and 'Stoeckerkopf' in the Northern Black Forest (under strong SO{sub 2}-impact until autumn 1987). On the site 'Edelmannshof', reduced immissions seem to exert positive effects on the phytohormone balance of the examined trees. Due to differences between individual tress subjected to the same treatment, it is not yet possible to decide, if there are really true effects on the phytohormone balance, which at 'Edelmannshof' is investigated over the period of only one year. On the site 'Stoeckerkopf' the obtained results point to a different behaviour of IAA-contents in needles of trees formerly subjected to SO{sub 2}-immissions and trees subjected to influences causing forest decline. (orig.).

  3. Changes of plant hormone levels in conifers subjected to immissions. Hormongehaltsaenderungen in Nadelbaeumen unter Immissionsbelastung

    Energy Technology Data Exchange (ETDEWEB)

    Frenzel, B.; Christmann, A. (Hohenheim Univ., Stuttgart (Germany, F.R.). Inst. fuer Botanik)


    The effect of reduced immissions on the phytohormone balance in conifers (ethylene, abscisic acid) is investigated on two sites: 1. The first site was under strong SO{sub 2}-impact until autumn 1987. 2. On the second site, spruce trees in open top chambers receiving charcoal-filtered air are compared with trees outside the chambers and trees in chambers receiving ambient air. Until now there are no systematic and significant differences seen in the hormone contents of the differently treated groups of trees. (orig./KG).

  4. Modification of fuel properties under thermal load; Veraenderung von Kraftstoffeigenschaften unter thermischer Belastung

    Energy Technology Data Exchange (ETDEWEB)

    Lau, Kornelia; Richter, Beate; Schuemann, Ulrike; Crusius, Svetlana; Streibel, Thorsten W.; Harndorf, Horst [Rostock Univ. (Germany). Abt. Analytische und Technische Chemie


    Diesel fuel without FAME and additives as well as rapeseed methyl ester were thermally stressed (150 C) at laboratory conditions. In the course of the performed study, the chemical composition of the fuels and possibly generated residues were analyzed with regards to the influence of oxygen availability and test duration. Therefore, chromatographic methods as GC-MS (Gas Chromatography-Mass Spectrometry) and HP-SEC (High Performance-Size Exclusion Chromatography) have successfully been applied. In the formed solid B 0-residues mainly oxygen-containing aromatic compounds were identified, while the biodiesel samples show mostly decomposition products of the biofuel like carbonic acids and aldehydes. In both fuels an increasing amount of compounds with high molecular weights was observed versus test duration. It is assumed that these components consist of oligo- and polymers with high oxygen content. However, this needs to be proved by further analytical methods. On the basis of these results prospective tailor-made additive packages for the stabilisation of fuels and the prevention of deposits in fuel injection systems, can be developed and applied. (orig.)

  5. Investigations on the sleep quality of electrosensitive residents near base stations unter homely conditions

    International Nuclear Information System (INIS)

    Leitgeb, Norbert


    In this study during a total of 214 nights sleep of 200 volunteers was analyzed. The investigated persons suffered from permanent and severe sleep disturbances and were deeply convinced that the environmental radiofrequency electromagnetic fields (RF EMF) caused their problems. The studies used mobile shields to compare the sleep quality with and without shield including a sham shield. For a predominant number the subjective conviction of volunteers that RF EMF pollution was the cause of their sleep disturbance could be falsified. The pooled analysis resulted in statistically significant placebo-effects for subjective sleep parameters. For 18% of volunteers it could be demonstrated that their belief in shielding improved sleep (placebo-effect). The sleep onset behavior of 9% of the volunteers was statistical significantly affected by RF EMF shielding. The investigations did not indicate adverse health effects of RF EMF emissions in general and from mobile telecommunication fields in particular

  6. Structure dynamics with regard to non-linear support behavior; Dynamische Strukturberechnung unter Beruecksichtigung nichtlinearen Lagerverhaltens

    Energy Technology Data Exchange (ETDEWEB)

    Driessen, W. [Technischer Ueberwachungs-Verein Nord e.V., Hamburg (Germany)


    Because of modifications to a feed-water line of a power plant structural calculations of the pipework were performed. As a result of a linear (modal) analysis very high restraint forces on the supports were calculated. In order to reduce conservatisms in the calculation the model was optimized with regard to the support stiffnesses and nonlinear behavior of slide bearings, guides and shock absorbers were taken into account. The main result of the non-linear analysis, which was performed by methods of direct-integration, was that nonlinearity yields evident differences in structural frequencies and in energy dissipation (damping) in comparison to the linear analysis. The high restraint forces on the supports became smaller for most of the supports but at some points the forces of the non-linear analysis were even higher. So the conservatism of the linear analysis is not fully valid for the whole structure. The relevance of the non-linear effects in dynamic piping calculations is shown by comparing the calculation result with measurements which were performed on structures in the plant. (orig.) [German] Im Rahmen der Aenderung der Speisewasserleitung einer Kraftwerksanlage wurde die Struktur neu berechnet. Die Analysen mit einem linearen Modell (modal), das ueblicherweise verwendet wird, ergaben hohe Lasten an Halterungen. Zum Abbau von Konservativitaeten wurde eine realistischere Modellierung durch die Beruecksichtigung des nichtlinearen Verhaltens der in der Anlage befindlichen Gleitlager, Fuehrungen und Stossbremsen in der Berechnung vorgenommen. Die Untersuchungen haben ergeben, dass durch die Nichtlinearitaet das Frequenzverhalten der Struktur und die Dissipation von Energie durch Reibvorgaenge wesentlich beeinflusst werden. Des Weiteren ist festzustellen, dass aus linearen Analysen nicht uneingeschraenkt konservative Ergebnisse gewonnen werden. Die Relevanz der Beruecksichtigung des nichtlinearen Lagerverhaltens bei einer dynamischen Strukturberechnung wird hier anhand eines Vergleiches mit einer der im Rahmen der In-Betrieb-Setzung des Kraftwerks gemessenen Verschiebungen aufgezeigt. (orig.)

  7. Purchasing motors in the light of full-cost calculation; Beschaffung von Motoren unter der Vollkostenbetrachtung

    Energy Technology Data Exchange (ETDEWEB)

    Mauchle, P.; Schnyder, G.


    This final report published by the Swiss Federal Office of Energy (SFOE) presents the results of a study made on how full-cost calculation can help define the optimal purchasing strategy for electrical drives. According to the authors, taking total life-cycle costs into consideration also guarantees economical operation and helps avoid unexpected service costs. The report describes the goals of the project and co-operation with industry and users. Factors influencing life-cycle costs are looked at in detail, including investments, installation and operator-training, operation and monitoring as well as maintenance and repair. Also, energy costs are looked at, as are environmental aspects and the disposal of drives taken out of service. The calculation of the economic feasibility of two types of motor is looked at. Sample Calls for Tender are presented and software for the evaluation of offers is briefly discussed.

  8. Electromobility for everyone? An industry electrified; Elektromobilitaet fuer alle? Eine Branche steht unter Strom

    Energy Technology Data Exchange (ETDEWEB)

    Winterkorn, Martin [Volkswagen AG, Wolfsburg (Germany)


    The electric car is one of the keys to achieving sustainable mobility - along with more efficient internal combustion engines, intelligent light-weight design and alternative sources of energy such as natural gas and next generation biogenic fuels. The Volkswagen Group is fully committed to electric mobility and is driv-ing the development of the electric car forward using its modular assem-bly strategy, the results of which can already be seen in the XL1 and the Golf blue-e-emotion. Before we are able to produce a practical, afforda-ble ''electric car for everyone,'' however, we face four major challenges: (1) developing the technology (especially the battery), (2) setting up an extensive standardized infrastructure, (3) ensuring an environmentally friendly source of electricity, and (4) driving down costs to a competitive level. All this makes electric mobility the challenge of the century - for the automotive industry and the European industrial community as a whole. It is an issue that concerns us all: manufacturers, suppliers, energy provid-ers, scientists and politicians alike. (orig.)

  9. Gesamtfahrzeugsimulation betriebsfestigkeitsrelevanter Manöver unter Berücksichtigung von Fahrwerkregelsystemen


    Brandes, Sebastian Jürgen


    In der Automobilbranche dient im Bereich der betriebsfesten Bemessung des Fahrwerks die Methode der Mehrkörpersysteme als Analyseverfahren zur Ermittlung von Bewegungen und Schnittgrößen an den Fahrwerksbauteilen. Eine der aktuellen Herausforderungen hierbei ist der zunehmende interdisziplinäre Charakter in Form von mechatronischen Fahrwerkssystemen. In rein virtuellen Entwicklungsphasen kann deren Einfluss auf die Fahrwerksbelastungen einzig anhand von Gesamtfahrzeugsimulationen ermittelt we...

  10. Zur Struktur des deutschen Satzes. Systematische Betrachtung der einzelnen Satzpositionen unter didaktischen Gesichtspunkten

    Directory of Open Access Journals (Sweden)

    Dieter Schmid


    Full Text Available Die Struktur, d.h. die Syntax des deutschen Satzes ist möglicherweise das komplexeste Thema der deutschen Grammatik. Es scheint, dass die meisten Fehler in diesem Bereich gemacht werden, und selbst Muttersprachler und Muttersprachlerinnen sind oft unsicher bzgl. der richtigen Wortstellung, insbesondere im Mittelfeld des Satzes. Dieser Aufsatz versucht, Deutschlehrern und -lehrerinnen eine Hilfestellung zu geben, indem er − aufbauend auf das topologische oder Stellungsfeldermodell von Erich Drach (vgl. Musan 2009: 10 − die wichtigsten Stellungsregeln für die verschiedenen Satzelemente angibt. Dies geschieht dadurch, dass alle möglichen „Positionen“ des deutschen Satzes systematisch untersucht werden, eine nach der anderen. The structure, i.e., the syntax of the German sentence is perhaps the most complex topic of German grammar. It seems that most mistakes are made in this area, and even native speakers are often not sure about the correct word order, especially in the middle-field of the sentence. This paper tries to help German teachers by giving them − based on the topological field model of Erich Drach − the most important rules about the correct positioning of the different elements of the sentence. It does so by examining systematically all the possible “positions” of the German sentence, one after the other.

  11. Das ritual der unterwerfung Stefan Nemanjas unter Maunel I. Komnenos (1172

    Directory of Open Access Journals (Sweden)

    Vučetić Martin Marko


    Full Text Available This article investigates the ceremonial of the subjection of the Serbian župan Stefan Nemanja to the Byzantine emperor Manuel I Komnenos (1172 and compares it with similar events of the period in question. It argues for a strong influence of western forms of conflict resolution (“deditio” in the ceremonial.

  12. Unternehmen unter der Dominanz der Finanzmärkte? Orientierungen aus sozialethischer Perspektive

    Directory of Open Access Journals (Sweden)

    Wolf-Gero Reichert


    Full Text Available There is a crucial change in the German financial system and listed corporations: The influence of the financial markets has grown in both fields. Subsequently, shareholders can enforce their interests more efficiently than other stakeholders (e.g. affiliates, NGOs, political communities, and employees. In the paper, diverse explanations for this development are presented and evaluated: disintermediation, financial market capitalism and ‘financialization’. Afterward, the topic is discussed in the perspective of theological ethics. Catholic Social Teaching features no financial ethics, but it includes some thoughts that offer an orientation for economic policy, the concept of an economy in service of the common good, the idea of a cooperation between state, market and civil society and socially anchored entrepreneurship as well as the conception of a serving financial economy. In conclusion, action perspectives are described.

  13. Methane synthesis under mild conditions for decentralized applications; Methansynthese unter milden Bedingungen fuer dezentrale Anwendungen

    Energy Technology Data Exchange (ETDEWEB)

    Schlueter, Michael [DBFZ Deutsches Biomasseforschungszentrum gGmbH, Leipzig (Germany); Roensch, Stefan


    It is a central aim of the German government to significantly reduce the emission of greenhouse gases in the next years. One possibility to reach this aim is the substitution of fossil fuels, especially natural gas, by fuels from biogenic sources (Bio-SNG). However, it is a drawback of Bio-SNG that the production costs are considerably higher than those of fossil natural gas. This work provides an approach to reduce the production costs of Bio-SNG. It is the aim to reduce the process parameters of the methane synthesis. At the same time, it has to be ensured that high methane yields are achieved even at those mild conditions. A procedure for the optimization of the methanation catalyst activity will be presented. If the catalyst is as active as possible even at mild conditions, it will be possible to produce Bio-SNG cost efficient even in small, decentralized scale.

  14. Modellierung der Energienachfrage unter Berücksichtigung urbaner Formen und individueller Zeitverwendung


    Braun, Stephan


    Die Zeitverwendung von Personen als auch die Ausgestaltung und Form von Siedlungen, in denen die Aktivitäten der Menschen stattfinden, stellen zwei wesentliche Einflussfaktoren auf die Energienachfrage wie auch auf die Möglichkeiten der Energiebereitstellung dar. Die Arbeit betrachtet die Zusammenhänge von urbaner Form und Energiebedarf und beschreibt den Wert der Nutzung von Zeitbudgetdaten im Kontext energiegeographischer Fragestellungen. In der praktischen Analyse werden CORINE Landcover D...

  15. Darstellung und Anwendung eines Bewertungsmodells im Rahmen des Controllings unter Beachtung der IAS/IFRS


    Volkmann, Sebastian


    Die zunehmende Etablierung des Shareholder Value-Gedankens in Europa, die Rechnungslegung nach IAS/IFRS in Kombination mit der Forderung nach Konvergenz des internen sowie des externen Rechnungswesens erfordern die vertiefte Kapitalmarktorientierung von Unternehmen. Entsprechend werden in dieser Arbeit Ansätze diskutiert, die wertorientierte Unternehmensführung mit Hilfe eines Bewertungsmodells zu unterstützen, um Implementierungslücken auf Ebene des Konzern- oder Geschäftsbereichscontrolling...

  16. Unter Krankheitsbedrohung. Vorbemerkungen zu einer Historischen Erforschung der Grossen Epidemien in der Österreichischen Bukowina

    Directory of Open Access Journals (Sweden)

    Harieta Mareci-Sabol


    Full Text Available Under Threat of Disease. Introduction to a Historical Study of the Great Epidemics in Austrian Bukovina Like natural disasters, wars and famine, epidemics recorded in Austrian Bukovina, affected people from all layers of society, leaving deep traces in memory and mentality. Considered as divine punishment or attributed to poverty, ignorance and lack of hygiene, they modeled different behaviors, reactions and attitudes, causing panic, tension, isolation, economic decline and, in some cases, depopulation. Information drawn from a variety of sources (from parish and civil registers to newspapers or memories provides an overview of morbid events recorded in Bucovina, from the late XVIIIth century to the early XXth century. Consecutive or recurrent episodes of plague, cholera, diphtheria, typhoid fever and typhus, measles, scarlet, smallpox and influenza have disrupted both rural and urban communities, imposing the imperial authorities, local officials, doctors, priests, teachers, and journalists to initiate some mechanisms for prevention, limitation or eradication of the disease.

  17. Einsatzmöglichkeiten des Hüttlin Kugelcoaters HKC 05-TJ unter Einbeziehung von Simulationen


    Wöstheinrich, Karin


    Ein Wirbelschichtgerät für den Labormassstab (Hüttlin Kugelcoater HKC 05-TJ) wird instrumentiert und mit Hilfe von Lactosegranulaten, die anschliessend tablettiert werden, validiert. Ein computergestütztes Simulationsprogramm wird entwickelt, mit dem Temperaturverläufe im Gerät während des Besprühens der Modellsubstanz Glaskugeln mit Wasser sowie das Schichtenwachstum der Glaskugeln nach Besprühen mit Lactoselösung berchnet werden. Vergleiche zwischen Modell und Experiment ergeben eine gute Ü...

  18. Entwicklungen in der Internen und der Externen Revision unter Berücksichtigung mittelgrosser Unternehmen


    Ruud, T F; Friebe, P; Isufi, S


    Aufgrund neuer gesetzlicher Bestimmungen sehen sich derzeit in der Schweiz nicht nur Grossunternehmen, sondern auch mittelgrosse Unternehmen mit Herausforderungen hinsichtlich des Risikomanagements und des Internen Kontrollsystems konfrontiert. Der Verwaltungsrat ist sowohl für das Risikomanagement als auch für das Interne Kontrollsystem verantwortlich. Allerdings werden die entsprechenden operativen Aufgaben delegiert, sodass für die Steuerung und Kontrolle der Risiken primär die Geschäftsle...

  19. Biosorption of heavy metals under anaerobic conditions. Final report; Biosorption von Schwermetallen unter anaeroben Bedingungen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Kreikenbohm, R.


    The precipitation of heavy metals as hydroxides is the standard technique for the decontamination of waste water streams polluted by these elements. On the other side, progress in research has been made concerning the biosorption onto dead biomass and bioprecipitation supported by physiologically active bacteria. As the aim of this study, a flexible strategy has been envisaged cleaning a waste water with definite heavy metal load underlying the process mentioned above. Suitable bacteria were enriched and the process was tested in a technical plant. As result, a very high efficiency of heavy metal elimination has been found. The field of application covered by the acquired process is identical with the whole range of the waste water streams polluted by heavy metals. In addition, a second stage may be necessary if there are any further contaminants to be removed. (orig.) [Deutsch] Bei der Reinigung von schwermetallhaltigen Abwaessern ist der derzeitige Stand der Technik gegeben durch die Neutralisationsfaellung als Hydroxide, waehrend in der Forschung damit begonnen wurde, Biosorption an devitaler Biomasse oder Biopraezipitation durch physiologisch aktive Bakterien zu untersuchen. Ziel des Vorhabens war die Ermittlung einer flexiblen Strategie fuer die Prozessfuehrung zur Abwasserreinigung bei vorgegebener Schadstoffbelastung auf der Basis des zuletzt genannten Prozesses. Dazu wurden geeignete Bakterien angereichert und das Verfahren in einer Technikumsanlage erprobt. Als Ergebnis wurde bei Zufuhr verschiedener Industrieabwaesser eine sehr hohe Effizienz in der Schwermetallelimination gefunden. Das Anwendungsgebiet des erarbeiteten Verfahrens erstreckt sich auf eine Vielzahl schwermetallbelasteter Abwaesser, wobei - je nach Art der weiteren Inhaltsstoffe - eine zusaetzliche Reinigungsstufe vor- oder nachgeschaltet werden muss. (orig.)

  20. Neue Medien unter der Organisationsperspektive. Eine empirische Untersuchung in der Weiterbildung

    Directory of Open Access Journals (Sweden)

    Richard Stang


    Full Text Available Gesellschaftliche Modernisierungsprozesse haben zu immensen Veränderungsprozessen auf allen Ebenen gesellschaftlichen Lebens geführt. Wirtschaft, Politik, Bildungswesen etc. sind als Gesamtsysteme davon betroffen, genauso wie die Institutionen und Organisationen, die in deren Rahmen agieren. Stehr (2000, S. 17 spricht davon, dass wir uns in einem „Übergangsstadium zwischen zwei Gesellschaftsformationen“ befinden und meint damit den Übergang von der „Industriegesellschaft“ zur „Wissensgesellschaft“, in der Wissen konstitutiv für die Gesellschaftsformation ist. Mit dem Bedeutungszuwachs der Ressource „Wissen“ gehen Prozesse der voranschreitenden gesellschaftlichen Ausdifferenzierung einher. So unterschiedlich die sozialwissenschaftlichen Analysen der Gesellschaftsformation und die daraus gefolgerten theoretischen Konstrukte auch sein mögen (vgl. u.a. Beck 1986, Castells 2001, Gross 1994, Schulze 1993, wird doch in einem besonderen Maße die Entwicklung von Technik, besonders der Informations- und Kommunikationstechniken (im Folgenden: Neuen Medien, als ein wichtiger Motor der gesellschaftlichen Veränderungsprozesse gesehen. Besonders Castells (2001 hat die gesellschaftliche, kulturelle und ökonomische Bedeutung der Neuen Medien in seiner Studie über die Netzwerkgesellschaft eindrucksvoll herausgearbeitet.

  1. The balancing experiment 1982 (BILEX '82) on the Unter Elbe River

    International Nuclear Information System (INIS)

    Michaelis, W.; Knauth, H.D.


    Temporal variabilities and spatial heterogeneities which are characteristic for tidal rivers considerably complicate the treatment of the both ecologically and economically important transport of suspended particulate matter and heavy metals. In 1982, a new concept to tackle this problem and the experimental and theoretical tools developed for its realization were, for the first time, tested in a concerted action. The instrumentation and the simulation models (current simulation model MOHNA, transport model FLUSS) applied have, in principle, proved a success. The experience gained during the campaign allows conclusions with regard to possible improvements to further increase the output. Extensive hydrographic, trace analytical and mathematical results are presented. They permit to judge the chance of success of future experiments of this kind and give hints how to proceed with further transport investigations under the complex conditions of a tidal river. (orig./HP) [de

  2. Waste management in underground mining. Colloquium; Abfallentsorgung im Bergbau unter Tage. Tagung

    Energy Technology Data Exchange (ETDEWEB)

    Frenz, W.; Martens, P.N.; Pretz, T. (eds.)


    The problem of underground waste management is reviewed from various aspects. Technical, legal and ecological problems are outlined with particular emphasis on the aspect of long-term safety. [German] Ziel unserer Veranstaltung ist, die Problematik der untertaegigen Abfallentsorgung aus den unterschiedlichsten Perspektiven zu beleuchten. Sowohl die technische als auch die rechtliche wie auch die oekologische Sichtweise werden aufgezeigt. In technischer Hinsicht werden der aktuelle Stand der Methoden und Fortentwicklungsmoeglichkeiten dargestellt und anhand konkreter Beispiele erlaeutert. Besondere Bedeutung hat dabei die Frage der Langzeitsicherheit. (orig.)

  3. Investigation of hydrogen recombination under natural convection conditions; Untersuchungen zur Wasserstoffrekombination unter Naturkonvektionsbedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Simon, Berno


    Passive Autocatalytic Recombiners (PAR) are installed inside the containment of nuclear power plants in order to prevent the build-up of flammable mixtures and to mitigate the effects of hydrogen deflagrations, which can occur in the event of a severe accident combined with the release of hydrogen. In order to simulate the operating behaviour of PARs, the computer program REKO-DIREKT is being developed at the Forschungszentrum Juelich in collaboration with the Institute for Reactor Safety and Reactor Technology at the RWTH Aachen. For the validation of the code, data from experimental facilities operated at Juelich are used. This work focusses on the analysis of the chimney effect through the PAR housing as well as the optimization of the chimney model of REKO-DIREKT. Therefore experimental investigations are carried out in the REKO-4 facility under natural convection conditions. This facility is equipped with numerous measuring devices, e.g. katharometers for in-situ measuring of the hydrogen concentration and the optical flow measurement technique Particle-Image-Velocimetry. In preliminary assessments the equipment is being qualified in order to determine the measurement accuracy. In the following experimental investigations, a small-scale PAR is used, that is built in a modular way allowing it to be equipped with different chimney geometries. The experimental results produce a database that shows the central correlation between the hydrogen concentration, the catalyst temperature and the inlet velocity. The results include the variation of the recombiner's chimney height and experiments at different operating pressures. After optimization of the chimney model, the simulation program is validated against experiments in the large-scale facility THAI in Eschborn, which have been performed subsequent to this thesis in the context of the OECD/NEA-THAI project. Finally, the influence of a downward-directed, near-wall flow on the operational behaviour of the small-scale PAR is investigated. The goal is to analyse, whether or not externally induced flow regimes are able to influence the start-up and operational behaviour of PARs. The results show that downward directed flow conditions may influence the operational behaviour and reduce the efficiency of a recombiner, if the flow through the chimney is not prevented by the outlet design.

  4. "Ich kam unter die Schweizer": Teaching Switzerland as a Multi-Ethnic Society (United States)

    Baumgartner, Karin


    This article describes a five-week module on "Switzerland as a multi-ethnic society" intended to counteract the popular image of Switzerland as a homogenous country concerned mostly with tourism, chocolate, and watches. Instead, the module treats Switzerland through topics such as the definition of identity in a multi-ethnic society, the…

  5. Ein Lehrbuch über Psychiatrie und Psychotherapie – Geschlechtsunterschiede unter der Lupe?

    Directory of Open Access Journals (Sweden)

    Isabella Heuser


    Full Text Available Anke Rohde und Andreas Marneros haben sich ein hohes Ziel gesetzt: ein übersichtliches Lehrbuch für Psychiatrie und Psychotherapie, das erstmals eine systematische Zusammenstellung des Wissens über geschlechtsspezifische Aspekte von Symptomatologie, Epidemiologie, Diagnostik, Pharmakotherapie und Psychotherapie psychischer Störungen enthält. Bemerkenswert ist dabei zweierlei: Erstens werden bei der Betrachtung geschlechtsspezifischer Unterschiede im Sinne der Genderforschung Frauen und Männer berücksichtigt, zweitens wird ein vollständiger Überblick sowohl über die häufigen und bekannten psychischen Störungen als auch die äußerst seltenen psychiatrischen Erkrankungen der gesamten Altersspanne gegeben. Wenngleich die Qualität der einzelnen Beiträge deutlich zwischen sehr differenzierter und leider auch für ein Handbuch eindeutig zu oberflächlicher Betrachtung variiert, liegt in dem Band ein weitgehend informatives Nachschlagewerk vor, das einen ersten Überblick über die geschlechtsspezifische Psychiatrie gibt.

  6. Physikochemische Untersuchung der Analyt – HKUST-1 Wechselwirkung unter Verwendung der inversen Gaschromatographie


    Münch, Alexander


    Die vorliegende Arbeit hat neben der Untersuchung der Synthese über den Controlled SBU-Approach von HKUST-1, ein poröses Kupfertrimesat, die Abscheidung dieses Metal-Organic Frameworks in dünnen Quarzglaskapillaren mit einer Länge von 10 bis 30 m und Innendurchmessern zwischen 0,53 und 0,25 mm zum Thema. Diese Säulen werden zur gaschromatographischen Trennung wie auch zur Bestimmung physikochemischer Kenngrößen, die den Adsorptionsvorgang verschiedener Analyten auf der HKUST-1 Oberfläche besc...

  7. Hoeren unter Wasser: Absolute Reizschwellen und Richtungswahrnehnumg (Underwater Hearing: Absolute Thresholds and Sound Localization), (United States)

    The article deals first with the theoretical foundations of underwater hearing, and the effects of the acoustical characteristics of water on hearing...lead to the conclusion that, in water , man can locate the direction of sound at low and at very high tonal frequencies of the audio range, but this ability is probably vanishing in the middle range of frequencies. (Author)

  8. Take control : Developing and testing novel treatments of substance dependence by targeting underlying neurocognitive processes

    NARCIS (Netherlands)

    Schulte, M.H.J.


    In this PhD project we investigated the effect of N-acetylcysteine (NAC) and working memory (WM) training on substance use, and associated changes in neural correlates of substance dependence. N-acetylcysteine is a glutamatergic agent that reduces craving by restoring the imbalance in the brain

  9. Kardiovaskuläres Risikofaktorenprofil und Herzinfarktrisiko nach dem PROCAM-Index bei Männern mittleren Alters unter besonderer Berücksichtigung der EKG-Veränderungen unter Belastungsbedingungen


    Kirchhof, M.R. (Monika)


    An 1248 Soldaten, wurde das kardiovaskuläre Risikofaktorenprofil bestimmt und das Herzinfarktrisiko nach dem PROCAM-Index ermittelt. Häufigkeit der Risikofaktoren: BMI?25kg/m2: 68,8%, positive Familienanamnese für Herzinfarkt 13,4%, Zigarettenrauchen 17,9%, körperliche Inaktivität 4,9%, systolischer RR?140mmHg: 50,9%, diastolischer RR?90mmHg: 41,4%, Gesamtcholesterin?5,2 mmol/l: 59,7%, HDL-Cholesterin?1,2 mmol/l: 33,5%, Gesamtcholesterin/HDL-Cholesterin-Quotient?4: 52,3%, LDL-Cholesterin ?4,...

  10. Optimization of a permanent magnet synchronous machine with respect to variable loads; Optimierung einer permanenterregten Synchronmaschine unter Beruecksichtigung von Lastspielen

    Energy Technology Data Exchange (ETDEWEB)

    Kreim, Alexander; Schaefer, Uwe [TU Berlin (Germany). Sek. EM4 Elektrische Antriebstechnik


    This article introduces a nonlinear optimization algorithm for mixed integer problems. The proposed algorithm is a trust region algorithm for an exact penalty function. The quadratic subproblem is used for the integration of discrete variables. This is done by a branch-and-bound approach. The application of the algorithm is shown by minimizing the losses of a permanent magnet synchronous machine. The machine is designed for use in hybrid and electric vehicles. It is shown how load cycles can be included into the optimization process. (orig.)

  11. Untersuchungen zur Involution der Vagina and Zervix beim Rind post partum unter Berücksichtigung der Zervix als Geburtshindernis


    Wehrend, Axel


    Ziel der vorliegenden Arbeit war es die physiologische Involution der Zervix im Vergleich zur Vagina beim Rind während der ersten zehn Tage post partum zu charakterisieren. Weiterhin sollte durch klinische Untersuchungen an Kühen nach Dystokie und einer retrospektiven Datenauswertung von Schwergeburten unterschiedlicher Tierarten der Anteil zervikal bedingter Geburtsstockungen im Interspeziesvergleich analysiert und Einflussfaktoren auf das Auftreten dieser Dystokieform sowie Störungen in der...

  12. Eine molekulardynamische Studie von amorphem SiO2 im Bulk und unter geometrischen Einschränkungen


    Geske, Julian


    Wasser und Silica sind beides Flüssigkeiten, die von hoher Relevanz sowohl in Anwendungen als auch in der Theorie sind. Lebewesen bestehen zu großen Teilen aus Wasser. Nicht nur dort kommt Wasser räumlich eingeschränkt vor, sondern ebenso in Gesteinen. Silica hingegen ist der bekannteste Glasbildner und hat daher vielfache Anwendungsmöglichkeiten in der Herstellung von (optischen) Gläsern. Ferner zeigen Silica und Wasser auf atomarer Ebene einen ähnlichen Aufbau. Beide Flüssigkeiten besitz...

  13. Underground- and surface technologies for joint flooding of gas caves; Unter- und Uebertagetechnik als Einheit zur Flutung von Gaskavernen

    Energy Technology Data Exchange (ETDEWEB)

    Raudonus, K.; Miersch, D. [UGS GmbH, Mittenwalde (Germany); Haensler, G. [Verbundnetz Gas AG, Leipzig (Germany)


    After 20 years of operation the oldes caves in the Bernburg storage VNG AG had to repair the facilities. This required flooding of the caves from the start. At the same time geomechanical reserves which were reevaluated on the basis of redimensioning could be used by deepening. Flooding and relocation of the displaced gas should not disturb gas storage and brine extraction. As one of the caves was filled with gas for the first time it was possible to use the brine. Flooding and gas filling were coupled. [Deutsch] Nach mehr als 20jaehriger Betriebszeit der aeltesten Kavernen auf dem Speicher Bernburg stand fuer die VNG AG die Aufgabe der Reparatur. Der Umfang der Arbeiten machte von Anfang an das vollstaendige Fluten der Kavernen erforderlich. Gleicheitig ergab sich dabei die Moeglichkeit, seinerzeit verbliebene geomechanische Reserven, die auf Basis einer auf aktuelle Erkenntnisse beruhenden Neudimensionierung bewertet wurden, durch Nachsolung auszuschoepfen. Die Heranfuehrung des Flutmediums und die Umlagerung des verdraengten Gases sollten ohne nachteilige Rueckwirkung auf den Gasspeicher- und Solegewinnungsbetrieb erfolgen. Da zu diesem Zeitpunkt eine Kaverne in der Phase der Gaserstbefuellung stand, konnte die ausgetragene Sole genutzt werden. Flutprozess und Gaserstbefuellung wurden somit im direkt gekoppelten Betrieb durchgefuehrt. (orig./MSK)

  14. Gerichtete Erstarrung von Al-Si und Al-Si-Mn Legierungen unter dem Einfluss von magnetischen Wechselfeldern


    Orth, Andreas


    In this work, samples of two alloys, Al-Si7 and Al-Si7-Mn1, are directionally solidified and their structure microscopically analysed. Thereby, the combined influence of induced current flow and intermetallic precipitates is of particular interest. A newly modified "Artemis" setup at the institute of material physics at the DLR in Cologne allows controlled solidification velocities of 30 to 240 micron/s under a constant temperature gradient of 3 K/mm. In using silica aerogels as part of the c...

  15. Lube-oil dilution of gasoline direct-injection engines with ethanol fuels; Schmieroelverduennung von direkteinspritzenden Ottomotoren unter Kaltstartrandbedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Kuepper, Carsten; Pischinger, Stefan [RWTH Aachen Univ. (Germany). Lehrstuhl fuer Verbrennungskraftmaschinen (VKA); Artmann, Chrsitina; Rabl, Hans-Peter [Hochschule Regensburg (Germany). Labor fuer Verbrennungsmotoren und Abgasnachbehandlung


    Ethanol fuel mixtures account for the majority of biofuels used worldwide. However, their properties make these fuels more difficult to use in cold conditions and especially when starting a cold engine. As part of the FVV research project 'Lubricant Dilution with Ethanol Fuels under Cold Start Conditions', the Institute for Combustion Engines (VKA) at RWTH Aachen University and the Combustion Engines and Emission Control Laboratory at Regensburg University of Applied Sciences have investigated the influence of the ethanol content in fuels on the dilution of the lubricating oil in modern direct-injection gasoline engines. (orig.)

  16. Fish populations under stress. The example of the Lower Neckar river; Fischpopulationen unter Stress. Das Beispiel des Unteren Neckars

    Energy Technology Data Exchange (ETDEWEB)

    Braunbeck, Thomas; Brauns, Annika; Keiter, Steffen [Sektion Aquatische Oekologie und Toxikologie, Univ. Heidelberg (Germany); Hollert, Henner [Inst. fuer Umweltforschung (Biologie V), Lehr- und Forschungsgebiet Oekosystemanalyse, Rheinisch-Westfaelische Technische Hochschule Aachen, Aachen (Germany); Schwartz, Patrick [Basel Univ. (CH). Mensch-Gesellschaft-Umwelt (MGU)


    Background, aim, and scope: Reports about declines or unusual structures of fish populations in native aquatic systems in Central Europe and North America are in sharp contrast to an obvious improvement of general water quality. The Neckar River may serve as an example of a formerly severely contaminated freshwater system in Southern Germany, the ecological situation of which could be substantially improved over the last three decades. Nevertheless, there are still deficits in the composition of the fish fauna, which cannot be explained by conventional chemical-analytical, hydromorphological and limnological methodologies. Therefore, in search of explanations for ecological deficits, ecotoxicological investigations with an increasing focus on sediment contamination have been performed along the Lower Neckar River over a period of 10 years. In addition to sediment tests, fish populations were screened for genotoxic and embryotoxic effects as well as alterations in the structure of central metabolic organs such as the liver. Materials and methods: Roach (Rutilus rutilus) and gudgeon (Gobio gobio) from the Lower Neckar River were studied with respect to histo- and cytological alterations of the liver as well as the induction of genotoxicity in liver, gut, gills and blood cells by means of the comet and micronucleus assays. At the same time, both native sediments and acetonic sediment extracts were tested for toxicity to zebrafish (Danio rerio) embryos and permanent fish cell cultures. Results: Massive disturbances of the liver ultrastructure indicate severe stress in the fish from the Lower Neckar River despite good supply of nutrition. Both cyto- and embryotoxicity tests document a considerable toxic potential of sediments from the Lower Neckar River, and results of both the comet assay and the micronucleus test provide evidence of the presence of genotoxic agents in the sediments and their effects in fish. There has been no decrease of genotoxicity over the last 10 years. Discussion: Cytopathology in conjunction with general cyto- and embryotoxicity in fish from the Lower Neckar River contribute to a syndrome which cannot be neglected in the interpretation of abnormal compositions of fish populations. In comparison to weight-of-evidence studies at other river systems in Southern Germany, the Neckar is characterized by an elevated embryotoxic but by a relatively moderate genotoxic potential. Conclusions: The ecotoxicological burden of sediments at the Lower Neckar River is mirrored in the condition of native fish populations. A potential contribution of chemical contamination to the phenomenon of fish declines in aquatic ecosystems cannot be ignored. Recommendations and perspectives: The improvement of the ecological status of freshwater systems may not be abused as an argument to discontinue biological monitoring programs. Rather, continuous surveillance of the environment and in-depth investigations into the consequences of chemical contamination are indispensible. For an adequate appreciation of the ecological relevance of sublethal and/or specific biological effects, their impact on population structure and function remains to be elucidated further. For the identification of the chemical basis of biological effects, methodologies such as effect-directed analysis appear to be promising approaches. (orig.)

  17. Bedarf an medizinischer Psychologie in der Zahnheilkunde:eine wissenschaftliche Untersuchung unter Zahnärzten und Zahnmedizinstudenten


    Bastian, C. (Cordula)


    Die teilnehmenden Stichprobengruppen (52 Zahnärzte, 78 Studenten) wurden zu dem Thema "Bedarf an medizinischer Psychologie in der Zahnheilkunde" befragt, wobei eine Mehrheit von 89% aller Befragten eine psychologische Schulung eines Zahnarztes für wichtig erachtet. In Ermangelung einer ausreichenden psychologischen Vor- und Weiterbildung halten sich die befragten Zahnärzte und Studenten auch nicht für gut genug gerüstet, schwierige Situationen im Beruf adäquat zu meistern ("wenig bis durchsch...

  18. Characteristics and modeling of spruce wood under dynamic compression load; Charakteristik und Modellierung von Fichtenholz unter dynamischer Druckbelastung

    Energy Technology Data Exchange (ETDEWEB)

    Eisenacher, Germar


    Spruce wood is frequently used as an energy absorbing material in impact limiters of packages for the transportation of radioactive material. A 9m drop test onto an unyielding target is mandatory for the packages. The impact results in a dynamic compression load of the spruce wood inside the impact limiter. The lateral dilation of the wood is restrained thereby due to encasing steel sheets. This work's objective was to provide a material model for spruce wood based on experimental investigations to enable the calculation of such loading conditions. About 600 crush tests with cubical spruce wood specimens were performed to characterize the material. The compression was up to 70% and the material was assumed to be transversely isotropic. Particularly the lateral constraint showed to have an important effect: the material develops a high lateral dilation without lateral constraint. The force-displacement characteristics show a comparably low force level and no or only slight hardening. Distinctive softening occurs after the linear-elastic region when loaded parallel to the fiber. On the other hand, using a lateral constraint results in significantly higher general force levels, distinctive hardening and lateral forces. The softening effect when loaded parallel to the fiber is less distinctive. Strain rate and temperature raise or lower the strength level, which was quantified for the applicable ranges of impact limiters. The hypothesis of an uncoupled evolution of the yield surface was proposed based on the experimental findings. It postulates an independent strength evolution with deviatoric and volumetric deformation. The hypothesis could be established using the first modeling approach, the modified LS-DYNA material model MAT075. A transversely isotropic material model was developed based thereupon and implemented in LS-DYNA. The material characteristics of spruce wood were considered using a multi-surface yield criterion and a non-associated flow rule. The yield criterion uses linear interpolation of the strength of constrained and unconstrained spruce wood. Thus multiaxial stress states can be considered. The calculation of the crush tests showed the ability of the model to reproduce the basic strength characteristics of spruce wood. The effect of lateral constraint can be reproduced well due to the uncoupled evolution of the yield surface. On the contrary, the strength is overestimated for load under acute angles, which could be prevented using modified yield surfaces. The effects of strain rate and temperature are generally reproduced well but the scaling factors used should be improved. The calculation of a drop test with a test-package equipped with wood-filled impact limiters confi rmed the model's performance and produced feasible results. However, to create a verified impact limiter model further numerical and experimental investigations are necessary. This work makes an important contribution to the numerical stress analysis in the context of safety cases of transport packages.

  19. Gebrauch von Komplementärmedizin bei Patienten mit metastasierendem Melanom unter Therapie mit Ipilimumab innerhalb einer klinischen Studie. (United States)

    Huebner, Jutta; Mohr, Peter; Simon, Jan-Christoph; Fluck, Michael; Berking, Carola; Zimmer, Lisa; Loquai, Carmen


    In Deutschland wenden 40-90 % aller Krebspatienten Methoden der komplementären and alternativen Medizin (KAM) an. Bis dato gibt es kein Datenmaterial zum Einsatz der KAM bei Melanompatienten. Das Ziel unserer Studie war es, Daten über den Gebrauch, die Informationsquellen und Ziele von Patienten mit metastasierendem Melanom zu erfassen. Einhundertsechsundfünfzig Patienten aus 25 Studienzentren nahmen an der DecOG-MM-PAL Multibasket Studie teil. Die beteiligten Personen wurden auch gebeten, an einer Nebenstudie teilzunehmen, die ihren Gebrauch von KAM erfassen sollte. Dazu wurde während der Behandlung ein standardisierter Fragebogen zu genau festgelegten Zeitpunkten ausgeteilt. Insgesamt gingen 55 Fragebögen von 32 (21 %) Melanompatienten ein. Von diesen gaben 17 (53 %) ein Interesse an KAM an, und sieben (22 %) machten von KAM Gebrauch. Die Hauptinformationsquellen (31 %) waren Familienmitglieder und Freunde, gefolgt von Ärzten (19 %). Die Hauptgründe für die Anwendung von KAM waren die Stärkung des Immunsystems (41 %) und des Körpers (34 %). Nahrungsergänzungsmittel (Vitamine und Spurenelemente) wurden am häufigsten angewendet (28 %). Eine relativ hohe Anzahl an Patienten mit metastasierendem Melanom machte trotz Teilnahme an einer klinischen Studie von KAM Gebrauch. Wechselwirkungen könnten durch biologisch basierte KAM auftreten, und hier besonders bei immunmodulierenden KAM- Strategien. Um Risiken zu vermeiden, sollte die Kommunikation zwischen den Ärzten und den Patienten verbessert werden. © 2016 Deutsche Dermatologische Gesellschaft (DDG). Published by John Wiley & Sons Ltd.

  20. Der Einfluss von Alitretinoin auf die Lebensqualität bei Patienten mit schwerem chronischen Handekzem: FUGETTA - Beobachtungsstudie unter Praxisbedingungen. (United States)

    Augustin, Matthias; Thaçi, Diamant; Kamps, Anja


    Alitretinoin ist die einzige zugelassene Behandlung für schweres chronisches Handekzem (CHE), das refraktär gegenüber potenten topischen Corticosteroiden ist. Bei dieser Studie (FUGETTA) wurde die Wirksamkeit von leitliniengerecht angewendetem oralem Alitretinoin sowie dessen Einfluss auf die Lebensqualität (LQ) bei Patienten mit schwerem refraktärem CHE beurteilt. Multizentrische, offene, nichtinterventionelle Beobachtungsstudie, durchgeführt in Deutschland. Die Patienten wurden nach Ermessen ihres behandelnden Arztes mit Alitretinoin 10 mg oder 30 mg einmal täglich über maximal 24 Wochen behandelt. Die Wirksamkeit wurde anhand des Physician Global Assessment (PGA) und des Dermatology Life Quality Index (DLQI) bewertet. Zudem wurden unerwünschte Ereignisse (UE) erfasst. Die Studienpopulation bestand aus 658 Patienten (30 mg: n = 581; 10 mg: n = 77). Bei Beobachtungsbeginn litten die meisten Patienten (83 %) gemäß PGA an einem schweren CHE. Bei Beobachtungsende war das Handekzem bei 48 % der Patienten gemäß PGA vollständig oder fast vollständig abgeheilt (30 mg: 49 %; 10 mg: 43 %). Die mittlere Verbesserung des DLQI-Scores in Woche 24 betrug 58 % (30 mg: mittlere [SD] Veränderung gegenüber dem Ausgangswert -10,4 [8,04]) und 70 % (10 mg: mittlere [SD] Veränderung gegenüber dem Ausgangswert -10,8 [7,29]). Die Gesamtinzidenz von NW war niedrig und in den beiden Gruppen ähnlich. Alitretinoin führte zu einer schnellen, deutlichen Verbesserung der LQ bei Patienten mit schwerem CHE. © 2016 Deutsche Dermatologische Gesellschaft (DDG). Published by John Wiley & Sons Ltd.

  1. Möglichkeiten der Umsetzung einer Bildung für nachhaltige Entwicklung unter Verwendung kooperativer Lernformen im Geographieunterricht


    Stange, Sarah


    „Ohne Nachhaltigkeit gibt es keine gute Zukunft“ betonte die Bildungsstaatssekretärin Cornelia Quennet-Thielen in einem Interview Anfang Oktober (BMBF 2015). Sie erklärte weiter: „Und ohne Bildung bleibt Nachhaltigkeit nur eine Forderung.“ (ebd.) Genau zehn Jahre sind vergangen, seit die Vereinten Nationen die UN-Dekade für Bildung für nachhaltige Entwicklung, kurz BNE, ausriefen. Der Leitgedanken der Nachhaltigkeit sollte über die Bildung Eingang in die Alltagswelten finden. Nun ist die UN-D...

  2. Entwicklung einer Wahrnehmungstaxonomie für Lernkontexte unter Berücksichtigung unterschiedlicher Perspektiven und Öffentlichkeitsgrade

    Directory of Open Access Journals (Sweden)

    Anne Mock


    Full Text Available Due to digitalization learning settings are increasingly opened. Through such an opening access to new, further knowledge exchange partners is generated. In an exploratory study Steinert et al. (2015 showed that learners perceive these new knowledge exchange partners even without explicit instructions. The present study systematizes the approaches of this study and examines whether there are differences in the perception of cognitive and social awareness information as well as the strength respectively proximity of the exchange partners’ interrelations. Hence, based on the current state of research, an awareness taxonomy for formal learning settings was theoretically derived and empirically tested.

  3. Investigations of the surface tension of coal ash slags under gasification conditions; Untersuchungen zur Oberflaechenspannung von Kohleschlacken unter Vergasungsbedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Melchior, Tobias


    In the context of CO{sub 2}-emission-induced global warming, greenhouse gases resulting from the production of electricity in coal-fired power plants gain increasing attention. One possible way to reduce such emissions is to gasify coal instead of burning it. The corresponding process is referred to as Integrated Gasification Combined Cycle and allows for the separation of CO{sub 2} before converting a synthesis gas into electrical energy. However, further improvements in efficiency and availability of this plant technology are needed to render the alternative generation of electricity sensible from an economic point of view. One corresponding approach introduces hot gas cleaning facilities to the gasification plant which guarantee a removal of slag particles from the synthesis gas at high temperatures. The development of such filters depends on the availability of data on the material properties of the coal ash slags to be withdrawn. In this respect, the surface tension is a relevant characteristic. Currently, the surface tension of real coal ash slags as well as of synthetic model systems was measured successfully by means of the sessile drop and the maximum bubble pressure method. With regard to the sessile drop technique, those experiments were conducted in a gasification-like atmosphere at temperatures of up to 1500 C. Furthermore, the pressure inside the experimental vessel was raised to 10 bar in order to allow for deriving the influence of this variable on the surface tension. In contrast, maximum bubble pressure trials were realised at atmospheric pressure while the gas atmosphere assured inert conditions. For performing sessile drop measurements, a corresponding apparatus was set up and is described in detail in this thesis. Three computer algorithms were employed to calculate surface tensions out of the photos of sessile drops and their individual performance was evaluated. A very good agreement between two of the codes was found while the third one produces heavily scattering output. The measurement arrangement was run in an almost fully automated fashion which resulted in an immense amount of obtained surface tension data. Maximum bubble pressure experiments were conducted at the University of Osaka, Japan, on selected real ash samples. Due to a far longer time required for determining bubble pressures in comparison to taking drop pictures, only a small number of temperatures could be studied abroad. The results show the surface tension to be in the range from 200 mN/m to 500 mN/m which is in accordance with data taken from the literature. While three discrete temperature intervals of particular slag behaviour could be identified in sessile drop experiments, results of maximum bubble pressure trials suggest the surface tension to be lower under inert conditions compared to a reducing atmosphere. The outcomes generated in Japan additionally show a better agreement to surface tensions forecasted by model calculations. Fe{sub 2}O{sub 3} being considered to have a pronounced influence on slag characteristics is made responsible for such observations. As soon as pressure is applied, the surface tension is found to decrease significantly. In order to visualise the data obtained by means of the sessile drop technique, regression functions were employed that can be implemented into future design calculations on hot gas cleaning facilities. (orig.)

  4. Purchasing motors under consideration of full-cost pricing; Beschaffung von Motoren unter der Vollkostenbetrachtung (inklusive Permanentmagneterregte-Synchronmotoren) - Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Mauchle, P. [Schnyder Ingenieure AG, Huenenberg (Switzerland); Ritz, Ch. [Schnyder Ingenieure AG, Steg (Switzerland)


    This final report for the Swiss Federal Office of Energy (SFOE) takes a look at how full-cost pricing should be considered when purchasing electric motors. The authors consider it essential that the overall life-cycle costs are carefully considered. This also guarantees economical operation and enables users to avoid unexpected costs throughout the service life of the motor. The aim of this project was to provide industrial companies with suitable tools for calculating the overall life-cycle costs of motors at the time of their acquisition. These tools take the form of a sample 'Call for Tender' for motors along with software for calculating life-cycle costs. The factors involved, such as investment, installation costs, energy and environmental costs as well as operational, maintenance and disposal costs are examined.

  5. Neuronale Korrelate sozialer Interaktion beim Menschen und deren genetische Modulation unter besonderer Berücksichtigung des Spiegelneuronensystems


    Cordes, Andrea


    Die in vielen Lebenssituationen über Erfolg und Misserfolg, Sympathie und Antipathie entscheidende, unterschiedlich ausgeprägte Fähigkeit zur sozialen Interaktion ist Gegenstand neurowissenschaftlicher Untersuchungen. Die Versuche einer kortikalen Lokalisierung führten zur Beschreibung des Spiegelneuronensystems (SNS) in Hirnregionen, die sowohl beim Ausführen einer Aktion Aktivität zeigten als auch beim Beobachten derselben beim Gegenüber. Die stärkste Aktivierung dieser Areale wurde bei sch...

  6. Webpräsenz und Katalog unter einem Dach - Relaunch des Internetauftritts der SuUB Bremen


    Blenkle, Martin


    Der Erfolg der großen kommerziellen Internetanbieter zeigt, dass die Akzeptanz von Webdienstleistungen nicht nur von der Qualität sondern auch von der Präsentation der eigenen Dienste abhängt. Die Berücksichtigung von Erkenntnissen aus Usability Studien - klare einfache Strukturen und die Beschränkung auf das Wesentliche - machen Webangebote für Nutzer attraktiver. Die Online-Präsentation der komplexen Angebote wissenschaftlicher Bibliotheken ist allerdings bislang eher von bibliothekarischer...

  7. Die kausalen Verknüpfungsmittel des Deutschen und des Italienischen. Eine kontrastive Beschreibung unter formalem und funktionalem Aspekt

    Directory of Open Access Journals (Sweden)

    Ballestracci, Sabrina


    Full Text Available This paper is a contrastive description of the causal grammatical structures mentioned in five grammar books of German and four grammar books of Italian. The study is inspired by the results of a previous empirical research upon the causal connectors denn, weil, da in German and perché, poiché, siccome in Italian, which has demonstrated that there are many similarities, but without any complete correspondence between the analyzed German and Italian grammatical structures. The purpose of this work is to verify if this thesis holds true not only for a limited number of connectors, but also for the whole system of German and Italian causal structures. The study is based upon a broad concept of causality, seen as the question about "why and with which purpose do events und states happen and verify". It focuses not only on the grammatical structures which are traditionally defined causal, but also on final and conditional structures. The results of the research are summarized at the end of the paper with the expectation that they will be helpful to the didactic transmission of the German causal structures with Italian learners.

  8. Multidimensional method for the determination of control power considering uncertainties; Multidimensionales Verfahren zur Bestimmung des Regelleistungsbedarfes unter Beruecksichtigung von Unsicherheiten

    Energy Technology Data Exchange (ETDEWEB)

    Kays, Jan; Schwippe, Johannes; Waniek, Daniel; Rehtanz, Christian [Technische Univ. Dortmund (Germany). Lehrstuhl fuer Energiesysteme und Energiewirtschaft


    One of the most crucial constraints in operation of electrical power supply systems is the permanent balance between generation and load. Reserve power is held ready to be able to keep this balance also in case of the occurrence of unpredictable events like power plant outages or inevitable deviations of power injections from their predicted values. At this, the allocation of operating reserves is in the field of conflict between level of reliability and cost effectiveness. The amount of reserve generation capacity required in a control area heavily depends on prediction quality which constitutes the need for high quality predictions. This article is about the influence of prediction quality on the amount of reserve generation capacity required in a control area. The algorithms for the assessment of required reserve generation capacity known from literature use the variation calculus technique to account for the aforementioned unpredictable events and prediction errors. In this article, an extension to a convolution-based method is presented in which the uncertainties of the predictions are expressed using intervals. Each parameter is represented by an independent dimension, assuming statistical independence among them. This allows the distinct analysis of each parameter's influence on the amount of required reserve generation capacity. In addition, two methods for the visualization of the multi-dimensional results are presented, allowing a comprehensive analysis of the parameters' influences. Standard distribution functions are used to represent the parameters for the simulation and the results are shown as probability density functions of possible imbalances. Based on forecasts of the development of conventional power plants and regenerative generation in the years 2020, 2030 and 2040, the required control power of the future German transmission system is calculated. Influences and dependencies are identified with the extended convolution-based method. (orig.)

  9. Ein Mythos wird besichtigt. Le deuxième sexe von Simone de Beauvoir unter dem Mikroskop der Genderforschung

    Directory of Open Access Journals (Sweden)

    Lieselotte Steinbrügge


    Full Text Available Aus Anlass des 50jährigen Jubiläums der Erstausgabe von Das andere Geschlecht haben namhafte Wissenschaftlerinnen aus den Gebieten Philosophie, Biologie, Soziologie, Psychoanalyse, Geschichte und Literatur das fast tausendseitige Standardwerk des Feminismus einer akribischen Lektüre unterzogen und dabei versucht, jene Fragen zu rekonstruieren, auf die das Werk bei seinem Erscheinen im Jahr 1949 eine Antwort gab. Die zeitlos scheinende Formel von der Konstruiertheit weiblicher Identität sollte wieder in ihren ursprünglichen Entstehungszusammenhang gestellt, die Autorin aus der mythischen Ferne in die kritische Nähe gerückt werden. Diese kritischen Lektüren sind insgesamt gut dokumentiert und erlauben meines Wissens zum ersten Mal einen Einblick in die Beauvoirsche Werkstatt. Ein weiterer – ebenfalls von Ingrid Galster herausgegebener – Band dokumentiert die Reaktionen, die unmittelbar nach Erscheinen von Le deuxième sexe in der französischen Presse erschienen sind.

  10. Einkaufen unter veränderten Vorzeichen: Ausgewählte Erfolgsfaktoren des "Konsortial-Benchmarking Einkauf 2010"


    Kreysa, Jennifer


    Die Finanz- und Wirtschaftskrise hat für viele Unternehmen nicht nur das externe Einkaufsumfeld, sondern auch die internen Anforderungen an den Einkauf stark verändert. Für das diesjährige Konsortial-Benchmarking im Einkauf hat die Abteilung Technologiemanagement des Fraunhofer IPT gemeinsam mit einem hochkarätigen Industriekonsortium erneut die Vorgehensweisen und Ansätze besonders erfolgreicher Einkaufsabteilungen analysiert. Die Ergebnisse des Projekts geben nicht nur Aufschluss darüber, w...

  11. Anwendung des Traumatherapieverfahrens -Narrative Expositionstherapie- bei komplex traumatisierten Patienten unter besonderer Berücksichtigung des Schlafes


    Scharff, Anna-Lena


    Die Studie sollte aufgrund der häufigen Komorbidität einer Posttraumatischen Belastungsstörung mit der Borderline Persönlichkeitsstörung die Frage untersuchen, ob die Narrative Expositionstherapie einen Therapieerfolg bei komplex traumatisierten Patienten hat. Desweiteren sollte der Effekt der Therapie auf den Schlaf untersucht werden und eventuell prädiktive Zusammenhänge zwischen Schlafqualität und Therapieerfolg herausgestellt werden.

  12. Die Bedeutung von Infinitiv und Konjunktiv als Modi der Nicht-Mitteilung (unter besonderer Berücksichtigung des Italienischen

    Directory of Open Access Journals (Sweden)

    Pichler, Claudia

    Full Text Available The infinitive (also referred to as "Nennform" is positioned both formally and functionally between noun and verb. This so-called "intermediate status" and the pragmatic-communicative characteristics/functions resulting from it are described on the basis of a selection of Old Italian language samples. The study therefore examines the correlation between morphosyntactic and informational structure and focuses in particular on the morphosyntactic codification of thematic elements of expression. An accordance can be ascertained regarding the pragmatic-communicative characteristics/functions of the infinitive ( i.e. infinitive constructions of the type: definite article + infinitive + subject and the (thematic subjunctive.

  13. Abschätzung des Einflusses von Parameterunsicherheiten bei der Planung und Auswertung von Tracertests unter Verwendung von Ensembleprognosen (United States)

    Klotzsch, Stephan; Binder, Martin; Händel, Falk


    While planning tracer tests, uncertainties in geohydraulic parameters should be considered as an important factor. Neglecting these uncertainties can lead to missing the tracer breakthrough, for example. One way to consider uncertainties during tracer test design is the so called ensemble forecast. The applicability of this method to geohydrological problems is demonstrated by coupling the method with two analytical solute transport models. The algorithm presented in this article is suitable for prediction as well as parameter estimation. The parameter estimation function can be used in a tracer test for reducing the uncertainties in the measured data which can improve the initial prediction. The algorithm was implemented into a software tool which is freely downloadable from the website of the Institute for Groundwater Management at TU Dresden, Germany.

  14. The climate protection policy of the USA under president Obama; Die Klimaschutzpolitik der USA unter Praesident Obama

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, Wolfgang; Schenk, Olga [Forschungszentrum Juelich (Germany). Inst. fuer Energieforschung - Systemforschung und Technologische Entwicklung; Holtrup-Mostert, Petra [Transatlantic Networks - Foreign Policy Analysis, Koenigswinter (Germany)


    Barack Obama's slogan 'Are you ready for a Change?' may become the motto fo the future climate protection policy of the USA. While the USA is slowly beginning to play a more active role in this area, there are many political and institutional obstacles to overcome before the USA can become one of the big global players here. The authors analyse the status quo of the US climate protection policy in an attempt to indicate national and international perspectives of climate protection. (orig.)

  15. Energy policy under power. Alternatives of power generation in the acceptance test; Energiepolitik unter Strom. Alternativen der Stromerzeugung im Akzeptanztest

    Energy Technology Data Exchange (ETDEWEB)

    Scheer, Dirk [Univ. Stuttgart (Germany). Zentrum fuer interdisziplinaere Risiko- und Innovationsforschung (ZIRIUS); Forschungsinstitut Dialogik, Stuttgart (Germany); Konrad, Wilfried [Forschungsinstitut Dialogik, Stuttgart (Germany); Renn, Ortwin [Univ. Stuttgart (Germany). Lehrstuhl fuer Umwelt- und Techniksoziologie; Forschungsinstitut Dialogik, Stuttgart (Germany); Univ. Stuttgart (Germany). Zentrum fuer interdisziplinaere Risiko- und Innovationsforschung (ZIRIUS); Scheel, Oliver [Univ. Stuttgart (Germany). Lehrstuhl fuer Umwelt- und Techniksoziologie; Univ. Stuttgart (Germany). Zentrum fuer interdisziplinaere Risiko- und Innovationsforschung (ZIRIUS)


    As illustrated, both the quantitative and qualitative analysis of the evaluations of the examined seven electricity mixes the image of a rejection of nuclear and coal power with or without carbon capture (CCS) with simultaneous support for a renewable energy focus in the center of the transformation of the German systems on the electricity. Energy-political strategies that the electricity produced from renewable sources less space than concede the nuclear power and/or the coal found only in very rare and exceptional cases decidedly on consent. Conversely: if the way in which renewable energy production convincingly by high levels of the electricity mix of ecological power plants are expressed, reduced amounts of nuclear and coal power are accepted as a transitional phenomenon. The finding that actually can get unpopular technologies from the focus of criticism by an appropriate context design, also applies to the CCS technology. This makes it possible to identify some evidence that in the context of nuclear energy-free RE-mixes modern coal-fired power plants with CCS are not be refused per se. However, it should also be noted that CCS will be rejected as a supposedly extra evil often still more vehement than the ''familiar'' nuclear power. [German] Wie dargestellt wird weist sowohl die quantitative als auch die qualitative Analyse der Bewertungen der untersuchten sieben Strommixe das Bild einer Ablehnung von Atom- und Kohlekraft mit oder ohne Carbon Capture (CCS) bei gleichzeitiger Unterstuetzung fuer eine die Erneuerbaren Energien in den Mittelpunkt stellenden Transformation des deutschen Systems der Stromerzeugung auf. Energiepolitische Strategien, die der Elektrizitaetsgewinnung aus regenerativen Quellen weniger Raum einraeumen als der Atomkraft und/oder der Kohleverstromung treffen nur in sehr seltenen Ausnahmefaellen dezidiert auf Zustimmung. Umgekehrt gilt: Wird der Weg in die regenerative Energieproduktion ueberzeugend durch hohe Anteile von Oekokraftwerken am Strommix zum Ausdruck gebracht, werden reduzierte Mengen an Atom- und Kohlestrom als Uebergangsphaenomen akzeptiert. Der Befund, dass durch eine geeignete Kontextgestaltung eigentlich unbeliebte Technologien aus dem Fokus der Kritik geraten koennen, gilt auch fuer die CCS-Technologie. So lassen sich einige Hinweise identifizieren, dass im Rahmen eines kernenergiefreien EE-Mixes moderne Kohlekraftwerke mit CCS nicht per se zurueckgewiesen werden. Allerdings ist ebenfalls festzuhalten, dass CCS als vermeintlich zusaetzliches Uebel oftmals noch vehementer als die ''vertraute'' Atomkraft abgelehnt wird.

  16. Urknall im labor: das grösste experiment der welt entsteht bei Genf - unter der erde

    CERN Multimedia


    The Big Bang in the laboratory has only few more energy than two colliding mosquitoes. But if we put together billions of mosquitoes, a hell bursts out. For that, near Geneva the biggest experiment in the world is being built.(1,2 page)

  17. Wear behavior of carbon fiber/aluminium-composites during abrasive loading; Verschleissverhalten von Kohlenstoffaser/Aluminium-Verbunden unter abrasiver Beanspruchung

    Energy Technology Data Exchange (ETDEWEB)

    Wielage, B.; Dorner, A. [Technische Univ. Chemnitz (Germany). Lehrstuhl fuer Verbundwerkstoffe


    Abrasive wear resistance of aluminium is significantly improved by the reinforcement with a high volume percentage of carbon fibres. The wear of unreinforced aluminium after scratching by a diamond indenter can be described as pure microgrooving without any micro-chipping. After integration of 70 vol.-% carbon fibers the damage mechanism is considerably altered and a great amount of micro-chipping occurs. The abrasive wear is strongly influenced by the fiber orientation. The best wear resistance is observed when the fibers are orientated perpendicular to the wear surface. (orig.) [Deutsch] Die Verstaerkung von Aluminium mit einem hohen Volumengehalt endloser Kohlenstoffasern verbessert erkennbar dessen Resistenz gegenueber Abrasionsverschleiss. Der Verschleiss von unverstaerktem Aluminium infolge des Ritzens mit einem Diamantindenter erfolgt in Form von reinem Mikrofurchen ohne Spanbildung. Aufgrund der Integration von 70 Vol.-% Kohlenstoffasern wird eine erhebliche Zunahme des Schaedigungsmechanismus Mikrospanen festgestellt. Die Faserorientierung hat merklichen Einfluss auf die Sensibilitaet gegenueber Abrasion. Orientierung der anisotropen Kohlenstoffasern senkrecht zur Verschleissoberflaeche bedingt den geringsten Abrasionsverschleiss. (orig.)

  18. Prevention of Radiocontrast-Induced Nephropathy after Coronary ...

    African Journals Online (AJOL)

    ) in patients undergoing coronary angiography pretreated with N-acetylcysteine NAC plus saline hydration or saline hydration alone and to determine the association between various risk factors and RCIN. Methods: Patients were ...

  19. Diagnosis and Management of Trichothecene in the Swine Model (United States)


    dazemgrel, N- acetylcysteine , dimethyl sulfoxide, adenosi.le triphosphate (ATP), ATP combined with magnesium chloride (ATP-MgCll), ascorbic acid, and...did not prolong survival times at the dosages employed. These included diltiazem hydrochloride, dazemgrel, N- acetylcysteine , dimethyl sulfoxide...rats given a lathal dose of T-2 toxin. Myocardial depress -nt factor (MOF) may play an important role in the pathophyslology of circulatory shock (Lefer

  20. The Role of Oxidative Stress in Apoptosis of Breast Cancer. (United States)


    interleukin; MnSOD. manganese superoxide dismutase; NAC, N- acetylcysteine ; NEM, N- . ethylmeleimide: NF-xB nuclear factor-xB; PAPS, adenosine 3Y-phosphate 5...and an increase in a NF-xB reporter activity. N- acetylcysteine (NAC), a cysteine derivative and a Redox signaling 11 GSH precursor, protects against... depressed MnSOD gene expression and enzyme activity and increased levels of oxidized proteins (Flores et al., 1993). 03-Amyloid is a neurotoxic peptide


    Directory of Open Access Journals (Sweden)

    Hamburg Andrea


    Full Text Available Present study is the extension of a former study upon Erasmus programmes and students’ attitude towards opening perspectives at the University of Oradea, Romania (the author’s institution of affiliation and thus overtakes parts and results of it enriching them with findings and conclusions of an extended research. In a world of intensified cross-cultural relations it would be of great importance that young people ‒ especially those in academic environments ‒ gain some international experience, get to know during their college period other cultures and socio-economic systems than theirs. The Erasmus programmes of the European Committee could give them due to the financial support they suppose the opportunity to live the experience of studying or doing practice in a company abroad and thus to extend their personal and professional horizon. Romania takes part in Erasmus programmes since 1998 and in the last two and a half decades sent more than 38,000 students on Erasmus study or placement mobility. It is a nice number but still too little related to the overall number of Erasmus scholarships academic institutions could dispose of yearly. By conducting a comparative study upon the relation between offer and demand at corresponding faculties of three academic institutions, the above mentioned University of Oradea, University of Debrecen, Hungary and University of Prešov, Slovakia one may realize that the situation is quite similar in all the three academic environments: Erasmus programmes don’t represent in general main attraction for students. The analysed study domains are: technique, economics, medicine/pharmacy/health care, history, political and communication sciences. The most interested in an international experience, in Romania and Hungary at least according to data of some academic years, prove to be medicine students who applied 2013-2014 in Oradea for about 44% and 2011-2012 in Debrecen for 80% of the scholarships offered. Slovakian students studying health care at the University of Prešov are leading in this respect, as the number of applications exceeded in the period 2011-2014 that of the places available. Why economic students at all the three institutions show in general little or moderate interest for Erasmus programmes, we have tried to find out by means of a questionnaire pointing to the main causes for applying/not applying for an Erasmus scholarship. The answers given by the over 120 students questioned at each institution correspond partially with the author’s initial suppositions and show main directions for counterbalancing and actions to be taken to increase visibility, feasibility and popularity of Erasmus programmes among students.

  2. Process measuring techniques applying in-line and on-line analyzers. Betriebsmesstechnik unter Einsatz von in-line und on-line Analysatoren

    Energy Technology Data Exchange (ETDEWEB)

    Oehme, F; Jola, M


    The measurement and control of classical physical parameters such as pressure, temperature etc, is frequently not sufficient now for optimum process control in chemistry and associated fields. Only the use of inline and online analysers makes it possible to maintain the narrow limits of chemical reaction conditions. This ABC pocket book should help the user to select the best from the large number of available methods of measurement. Using 120 alphabetically arranged key words with many cross-references, it has a well-thought-out treatment of basic measurements and descriptions of apparatus, and on the other hand, it contains selected typical examples of applications. Width rather than depth of information is aimed at. The electrochemical methods discussed are potentiometry (pH value, redox potential, direct potentiometry with ion selective electrodes), conductometry (measurement of conductivity) and voltammetry (polarography, ammetry). Photometry, the measurement of density, the refractive index, surface tension and viscosity are described. From the large number of applications, the examples of textile processing, the cellulose and paper industry, the surface treatment of materials and petrochemicals are discussed. Special attention is paid to the treatment of service water, drinking water and boiler feed water. In connection with questions of work and environmental protection, emission control and the treatment of domestic and industrial waste water are described. With 119 figs.

  3. Man's influence on climate with special consideration of energy technology. Problem description - information - opinions. Klimabeeinflussung durch den Menschen unter besonderer Beruecksichtigung der Energietechnik. Problemdarstellung - Wissensvermittlung - Meinungsbildung

    Energy Technology Data Exchange (ETDEWEB)


    This book contains the lectures of a meeting in December 1988, organized by the Association of German Engineers (VDI). The eleven contributions deal with the possible climatic changes caused by the increase in CO/sub 2/ of the atmosphere by the combustion of fossil fuel. A survey of the climatic fluctuations of the past is given and the principles of climate models are described. Other contributions deal with the influence of the cryosphere and the clouds on the climate and with the influence of aerosols on the radiation balance. The coupling of climate and biosphere and chemical reactors of trace gases in the atmosphere are also presented. Some important measures for the reduction of the increase of CO/sub 2/ by changes in the energy policy are discussed. (KW).

  4. Optimizing the gear efficiency under consideration of thermal optimisation strategies and customer-specific load conditions; Optimierung des Getriebewirkungsgrads unter Beruecksichtigung thermischer Optimierungsstrategien und kundenspezifischer Lastkollektive

    Energy Technology Data Exchange (ETDEWEB)

    Inderwisch, Kathrien; Kuecuekay, Ferit [Technische Univ. Braunschweig (Germany). Inst. fuer Fahrzeugtechnik


    Nowadays, the automotive industry have been received more attention to improve the transmission efficiency. Most of the researches have been concentrated on development and optimisation on transmission actuators, shifting elements, bearings, lubricants or lightweight constructions. Due to the low load requirements and associated low efficiencies for transmissions in driving cycles the transmissions cause energy losses which cannot be neglected. Two main stategies can be followed up for the optimisation of transmission efficiency. At first the efficiency benefit of transmissions through optimisation of hardware components will be presented. The second possibility is the representation of an optimal thermal management especially at low temperatures. Warming-up the transmission oil or transmission components can increase the efficiency of transmissions significantly. Techniques like this become more important in the course of electrification of drive trains and therefore decreased availability of heat. A simulation tool for calculation and minimisation of power loss for manual and dual-clutch transmissions was developed at the Institute of Automotive Engineering and verified by measurements. The simulation tool calculates the total transmission efficiency as well as the losses of individual transmission components depending on various environmental conditions. In this paper, the results in terms of increasing the efficiency of transmissions by optimisation of hardware components will be presented. Furthermore, the effects of temperature distribution in the transmission as well as the potential of minimising loss at low temperatures through thermal management will be illustrated. (orig.)

  5. Rehabilitation of the natural gas field Thoense with wet transport of natural gas; Sanierung des Erdgasfeldes Thoense unter Anwendung des Erdgasnasstransports

    Energy Technology Data Exchange (ETDEWEB)

    Neumann, W.; Gerhartz, B.; Schmitt, D.; Uphoff, T. [BEB Erdgas und Erdoel GmbH, Hannover (Germany)


    Natural gas has been extracted the gas field of Thoense situated at the Northern fringe of the city of Hannover for over forty years. At all 10 bore sites the gas was dried with glycol dryers. While dealing with the problem of hydrocarbon emissions from the dryers it became evident, that some of the very old plants had to be rehabilitated. BEB operates the Thoense field on behalf of Elwerath/Brigitta/Deutz. An integrated concept, based on wet transport of natural gas, was used to remove the emission of hydrocarbons and rehabilitate the surface plants in order to cut operating costs. The field at Thoense is now economically efficient enough to continue operations for a long time. [Deutsch] Aus dem am nordoestlichen Stadtrand Hannovers liegenden Gasfeld Thoense wird seit ueber vierzig Jahren Erdgas gefoerdert. Die Gastrocknung erfolgte dort in ueblicher Weise mit Glykoltrocknungsanlagen an allen zehn Bohrungen. Bei der Bearbeitung des damit verbundenen Problems der Kohlenwasserstoffemissionen aus den Trocknungsanlagen wurde der Sanierungsbedarf an den z.T. sehr alten Anlagen deutlich. Das Feld Thoense wird von BEB fuer das Konsortium Elwerath/Brigitta/Deutz betrieben. Es wird gezeigt, wie mit einem integrierten Konzept auf der Basis des Erdgasnasstransportes die KW-Emissionen beseitigt wurden und gleichzeitig eine betriebskostensenkende Sanierung der obertaegigen Anlagen durchgefuehrt werden konnte. Damit wurde die wirtschaftliche Voraussetzung geschaffen, die Gasversorgung aus Thoense noch lange aufrecht erhalten zu koennen. (orig.)

  6. MR tomography of hemophilic osteoarthropathy with special reference to synovial and chondrogenic alterations. MR-Tomographie der haemophilen Osteoarthropathie unter besonderer Beruecksichtigung der synovialen und chondrogenen Alterationen

    Energy Technology Data Exchange (ETDEWEB)

    Erlemann, R. (St. Johannes-Hospital, Inst. fuer Radiologie, Duisburg-Hamborn (Germany)); Pollmann, H. (Westfaelische Wilhelms-Univ. Muenster, Kinderklinik, Abt. Haematologie und Onkologie (Germany)); Vestring, T.; Peters, P.E. (Westfaelische Wilhelms-Univ. Muenster, Inst. fuer Klinische Radiologie (Germany))


    52 knee and ankle joints of hemophiliacs were examined by MRI using FLASH and FISP-3-D sequences; and the degree of synovial hypertrophy and of cartilage destruction were assessed. Findings of synovial hypertrophy varied between thin membranes and tumorous tissue destroying the joint cartilage. Degree of cartilage destruction varied between focal signal decrease and total loss. In spite of recurrent joint bleedings no synovial or cartilaginous changes were seen in 31% and 29% of joints, respectively. Changes were more frequently seen and degree was more marked in the ankle than in the knee joints. With the exception of cysts, osseous destruction was more obvious with radiographs. MRI is suitable for the investigation of joints of hemophiliacs showing no osseous destruction. (orig.).

  7. Ambulant transbrachial 4-french-arteriograhy with special reference to the aorto-femoral territory. Ambulante transbrachiale 4-French-Arteriographie unter besonderer Beruecksichtigung der aortofemoralen Strombahn

    Energy Technology Data Exchange (ETDEWEB)

    Spindler-Thiele, S. (Klinikum Ingolstadt (Germany). Inst. fuer Strahlendiagnostik); Schmitt, R. (Klinikum Ingolstadt (Germany). Inst. fuer Strahlendiagnostik); Helmberger, T. (Klinikum Ingolstadt (Germany). Inst. fuer Strahlendiagnostik); Pogan, J. (Klinikum Ingolstadt (Germany). Inst. fuer Strahlendiagnostik); Gullotta, U. (Klinikum Ingolstadt (Germany). Inst. fuer Strahlendiagnostik)


    Arteriograms were carried out on 176 patients using 4-F catheters through a transbrachial approach. Criteria for exclusion from the series were injuries to the upper limb, hemiparesis or poor pulses in the presence of a normal femoral pulse. There were no local vascular complications requiring treatment. On two occasions the brachial artery could not be punctured. Acute posterior cerebral infarction was the only serious complication (0.5%). 67% of the patients were examined on an outpatient basis and this did not appear to increase the risk of complication. We regard this as a suitable method for demonstrating the pelvic and lower limb arteries on ambulant patients. (orig.)

  8. Lithium for future technologies. Demand and supply with special regard to electro-mobility; Lithium fuer Zukunftstechnologien. Nachfrage und Angebot unter besonderer Beruecksichtigung der Elektromobilitaet

    Energy Technology Data Exchange (ETDEWEB)

    Angerer, Gerhard; Marscheider-Weidemann, Frank; Wendl, Matthias; Wietschel, Martin


    The availability of the necessary commodities for electric vehicles with regard to their high market growth predictions is currently a frequent theme of controversial discussions. At this the mineral Lithium, which is used in vehicle batteries, is in the point of interest. An estimation of the worldwide Lithium (Li)-consumption and -supply to the year 2050 was established in this study with the goal of analysing whether significant resource shortages are in fact to be expected. A calculation model was therefore developed, which estimates the future Li-demand using the 2008 consumption of the main Li-consumers such as the glass- und ceramics- industry or lubricants and their respective industry growth rate predictions. As the vehicle battery market can dominate the future Li-demand its development was examined in detail using two market penetration scenarios. Moreover the amount of secondary Lithium which can be recovered through recycling was determined via collection and recycling-quotas. In the, concerning the future number of electric vehicles, moderate Pluralismus-scenario about 51 % of the world Lithium-reserves will be consumed in 2050 under consideration of the employment of recycled material. At this a rather low assumption of the Li-reserves was applied. The gained amount of secondary Lithium covers about 25 % of the total Li-demand which underlines the importance of recycling. In the second scenario which displays a quick and thorough market penetration of electric vehicles the Lithium-reserves are already exhausted in 2045 in spite of the recirculation of secondary-material. The reserves comprise the Li-supplies within the lithosphere which can be recovered under current commodity prices and state-of-the-art technical possibilities. Based on a (USGS, 2009) estimation they amount to 6 Mio. t. Lithium. The resources on the other hand also contain known sources which can't be economically exploited at the moment. They amount to approx. 14 Mio. t Lithium according to the above source whereas other noted sources, with (Roskill, 2009) among them, state them at 28 - 30 Mio. t Lithium. With regard to the geological resources of the USGS estimation just about 20 % of the geological occurrence is exploited in the Pluralismus-scenario, 49 % in the Dominanz-scenario. The bottom line of this study is that even under extreme assumptions a Lithiumshortage is not to be anticipated within the next four decades. On the other hand the fact that the world Li-reserves are concentrated on very few countries of which some currently don't belong to geopolitically stable regions (e.g. Bolivia) has to be considered. Furthermore the mining of Lithium intervenes in up to now mostly untouched ecosystems such as the salt-lakes in South America and China. For the preservation of the commodity base a working recycling system for Lithium has to be established in time. In a long-term perspective the development of alternative battery systems has to be promoted further. Additionally other raw materials have to be analysed on possible future shortages in the context of electro-mobility. Cobalt, copper,indium or neodymium for example are commodities which could cause trouble in future.

  9. Tax reform for ecologic reasons: The basic legal situation in Germany. Expert opinion; Steuerreform unter oekologischen Aspekten: Rechtliche Rahmenbedingungen. Gutachterliche Stellungnahme

    Energy Technology Data Exchange (ETDEWEB)

    Birk, D.; Eckhoff, R. [Muenster Univ. (Germany). Inst. fuer Steuerrecht


    A model simulating the outcome of an ecologic tax reform in Germany is presented and discussed. The model was elaborated by Deutsches Institut fuer Wirtschaftsforschung (DIW) and Finanzwissenschaftliches Forschungsinstitut (Fifo) of Cologne University. The model sets out the framework and scope of such a tax reform, as well as individual policy instruments, such as introduction of tax on energy consumption, an increase of the mineral oil tax, introduction of a road-use tax, introduction of new and increase of existing charges for waste management, a modification and increase of the sewage levy, and in the long run, introduction of a tax on land consumption by built environments. An analysis of the redistributional effects of the theoretical tax on energy consumption together with the increased mineral oil tax revealed that the prorated expenses for energy in relation to total incomes and revenues are declining with rising income. Especially the prorated expense for automotive fuels shows a particularly steep rise, which is calculated by the model to decline again only within the group of households with a very high income. The financial burden effect of the energy tax in relation to the available income and revenues is described by a regressive curve similar to the energy expenses curve, other than with the tax burden curve due to the increased mineral oil tax, which is calculated to have a regressive effect only at higher income levels, i.e. from a private household net income of DM 4000. Considering however the tax burden effects resulting from both the energy tax and the increased mineral oil tax, for all private hoseholds, a regressive effect is expected to set in as from a household income of DM 1000. (orig./CB) [Deutsch] Das vom Deutschen Institut fuer Wirtschaftsforschung (DIW) und vom Finanzwissenschaftlichen Forschungsinstitut der Universitaet zu Koeln (Fifo) entworfene Modell einer oekologischen Steuerreform sieht die Einfuehrung einer Energiesteuer, eine Erhoehung der Mineraloelsteuer, die Einfuehrung von Abgaben fuer die Strassenbenutzung, die Einfuehrung oder Erhoehung von Abfallabgaben, die Umgestaltung und Erhoehung der Abwasserabgabe und laengerfristig die Einfuehrung einer Flaechenversiegelungsabgabe vor. Eine Analyse der Verteilungswirkung der geplanten Energiesteuer und der Erhoehung der Mineraloelsteuer ergibt, dass der Anteil der Energieausgaben an den ausgabefaehigen Einkommen und Einnahmen mit steigendem Einkommen deutlich abnimmt. Insbesondere der Anteil der Ausgaben fuer Kraftstoffe steigt jedoch und geht erst bei Haushalten mit sehr hohem Einkommen wieder zurueck. Die Belastungswirkung der Energiesteuer bezogen auf die ausgabefaehigen Einkommen und Einnahmen nimmt einen aehnlich regressiven Verlauf wie die Energieausgaben. Dagegen entfaltet die sich aufgrund der Mineraloelsteuererhoehung ergebende Steuerbelastung erst ab einem Haushaltsnettoeinkommen von 4.000 DM eine regressive Belastungswirkung. Das verhindert jedoch nicht, dass sich fuer Energiesteuer und Mineraloelsteuer insgesamt ab einem Haushaltseinkommen von 1.000 DM eine regressive Belastungswirkung ergibt. (orig.)

  10. Dosisabhängiger Anstieg von Knochenformatinsmarkern in Serum und Knochen unter Zink-Supplementation - eine Untersuchung an der erwachsenen weiblichen Maus

    Directory of Open Access Journals (Sweden)

    Dimai H-P


    Full Text Available Zink (Zn ist eine essentielles Spurenelement. Es spielt eine wichtige Rolle in zahlreichen metabolischen Prozessen und wird auch für das normale Knochenwachstum benötigt. Ziel der vorliegenden Untersuchung war es, den Effekt von Zn auf a die Aktivität der alkalischen Phosphatase (ALP in Serum und Knochen, b das Serum-Osteocalcin, c die Tartrat-resistente saure Phosphatase (TRAP im Knochen, sowie d den Protein-Gehalt des Knochens darzustellen. Erwachsene weibliche Swiss-Webster-Mäuse erhielten entweder eine Zn-freie Diät, oder eine Diät, welche einen normalen, oder einen 2-, 3-, 4-, oder 5-fach höheren Zn-Gehalt als die Normal-Diät hatte. Die Diät wurde für 2, 4, oder 6 Wochen verabreicht. Es zeigte sich eine positive Korrelation zwischen der Menge des mit der Nahrung aufgenommenen Zn und der Serum-ALP (4 und 6 Wochen, p 0,001, der tibialen ALP (2, 4 und 6 Wochen, p  0,03, sowie dem tibialen Protein (2,4 und 6 Wochen p 0.001; weiters zeigte sich eine negative Korrelation zwischen Zn und tibialer TRAP (2, 4 und 6 Wochen, p 0,001. Kovarianzanalysen zeigten überdies, daß die Aktivität der Serum-ALP, der tibialen ALP und der tibialen TRAP, sowie die Menge des tibialen Proteins sowohl durch die Zn-Dosis (p 0,005, als auch die Dauer der Therapie (p 0,03 beeinflußbar sind. Zn zeigte auch einen signifikanten Einfluß auf den Serum-Osteocalcin-Spiegel (p 0,03 bei 3 x höher Zn-Menge als in der Normaldiät. In einer ergänzenden Studie zeigte sich auch, daß Zn zu einem dosisabhängigen Anstieg der ALP und des Proteins, und zu einem signifikanten Abfall der TRAP in murinen osteoblastenähnlichen MC3T3-E1 Zellen führt. Insgesamt kann aus den Ergebnissen der vorliegenden Studie geschlossen werden, daß eine schrittweise Erhöhung des Zn-Gehaltes der Nahrung zu einem entsprechenden Anstieg der ALP-Aktivität von Serum und Knochen führt. Da in dieser Studie klar gezeigt werden konnte, daß Zn auch zu einem Abfall der TRAP-Aktivität in osteoblastenähnlichen Zellen führt, sollte künftighin ein Absinken der TRAP-Aktivität im Knochen nicht als Zeichen einer Knochenresorptions-Hemmung interpretiert werden.

  11. Frozen soil and snow cover with respect to the hydrological land-surface behaviour; Gefrorener Boden und Schneebedeckung unter besonderer Beruecksichtigung des hydrologischen Verhaltens der Landoberflaeche

    Energy Technology Data Exchange (ETDEWEB)

    Warrach, K. [GKSS-Forschungszentrum Geesthacht GmbH (Germany). Inst. fuer Atmosphaerenphysik


    Investigations of the water and energy cycle in the climate system using atmospheric circulation models require a proper representation of the land surface. The land-surface model SEWAB calculates the vertical exchange of water and energy between the atmosphere and the land-surface. This includes the calculation of runoff from the land-surface into the rivers and of the vertical heat and water fluxes within the soil. The inclusion of soil freezing and thawing and the accumulation and ablation of a snow cover in SEWAB is introduced. Additionally changes in the runoff calculation such as the inclusion of the TOPMODEL-approach to consider orographic effects are made. Applications carried out for various regions of North America show good agreement between model results and measurements. (orig.)

  12. Assessment of laserbeam-welded tubular overlap joints made of magnesium and aluminium under multiaxial loading; Bemessung laserstrahlgeschweisster Ueberlappverbindungen aus Magnesium und Aluminium unter mehrachsiger Beanspruchung

    Energy Technology Data Exchange (ETDEWEB)

    Exel, Nora; Wiebesiek, Jens; Sonsino, Cetin Morris [Fraunhofer-Institut fuer Betriebsfestigkeit und Systemzuverlaessigkeit (LBF), Darmstadt (Germany); Hanselka, Holger [Fraunhofer-Institut fuer Betriebsfestigkeit und Systemzuverlaessigkeit (LBF), Darmstadt (Germany); Technische Univ. Darmstadt (Germany). Fachgebiet ' ' Systemzuverlaessigkeit und Maschinenakustik' '


    The present paper compares the wrought light-metal alloys AlMg3.5Mn and MgAl3Zn1 based on the fatigue behaviour of laserbeam-welded overlapped tubular joints. The experiments were carried out under pure axial, pure torsional and combined proportional as well as nonproportional loading. The test results were assessed by applying the notch stress concept with a reference radius of r{sub ref} = 0,05 mm and compared with allowable stresses taken from a standard. Finally, two stress based multiaxial hypothesis are compared to each other based on the test results. (orig.)


    Directory of Open Access Journals (Sweden)

    Kasilda Bedenk


    Full Text Available ABSTRACTPurpose: The article is focused on the comparison of phrasemes in Grimm's fairy tale Hans in Luck (Hans im Glück translated into Slovene by six different translators in the period from 1857 to 1993. The author wants to establish whether the translators recognized all the phrasemes in German and determine whether they were translated in the target language word by word or by the use of Slovene phrasemes. She wants to identify the influence of German on the formation and dissemination of phrasemes in Slovene and to define strategic decisions of translators in searching the best translation solutions.Methodology/Approach: The author selected six different translations of the fairy tale Hans in Luck (Hans im Glück from German to Slovene published in 1937 containing 44 phrasemes. She was especially interested in phrasemes in the so called narrow meaning and in the pragmatic or communication phrasemes. She selected analysed and compared these phrasemes. She studied whether the Slovene Literary Language Dictionary and the corpus GigaFida contain these phrasemes as well as the frequency of their use.Results: In most cases the translators recognized the phrasemes and incorporated them in their translations as multi word units containing phraseological information. They tried to find original solutions, i.e. established Slovene phrasemes which is especially evident in older translations. It is interesting that the translator of the latest (and the first complete Slovene translation of Grimm's Children's and Household Tales from 1993 often searched new solutions which in most cases were not better than those of her predecessors. The latest translation of the phrasemes is thus more literal, calque and leading to the lack of clarity of certain parts of the text.Research limits: The research is limited to a rather small sample of translations from German trying to establish the understanding and strategy of individual translators and to determine the semantic impact of translations of one of the most frequently read type of literature.Originality/applicability: The research serves as an instrument to identify the transmission of cultural patterns and related linguistic expressions and images by which translations are shaping our linguistic consciousness.ZUSAMMENFASSUNGDie Autorin interessiert der kulturelle und sprachliche Einfluss der Grimm’schen Märchen bzw.ihrer Übersetzungen auf die slowenische Sprache und Kultur. In ihrem Beitrag zur diachronen Betrachtung verschiedener slowenischer Übersetzungen von Phraseologismen im Märchen Hans im Glück (KHM 83 ging sie der Frage nach der Angemessenheit der Übersetzungen, der Strategie des jeweiligen Übersetzers, dem heutigen Gebrauch bzw. der Bekanntheit der duch die Übersetzung ev. neueingeführten Phraseologismen und der sprachlichen Weiterentwicklung der einzelnen festen Wortverbindungen nach und erwägt, ob die letzte Übersetzung auch die beste« war.Die Untersuchung war lediglich exemplarisch, da die einbezogenen Beispiele keinesfalls eine repräsentative Menge darstellen.Ihre Schlussfolgerung ist, dass die früheren Märchenübersetzungen überraschend gut waren. Die Übersezter entwickelten im 19. und im 20. Jh. ein hohes Sprachbewusstsein, indem sie Lehnübersetzungen vermieden, wenn es nur möglich war. Die letzte (vollständige Übersetzung aus 1993 von Polonca Kovač ist eine eher ausgangstexttreue Übersetzung. Die Übersetzerin wollte ihre Treue zum Original besonders auf der Sprachebene zeigen, indem sie vieles wortgetreu übersetzte, Lehnübersetzungen und Lehnphraseme sowie veraltete und weniger bekannte dialektale Ausdrücke gebrauchte, was bestimmte Abweichungen von den gegenwärtigen übersetzunswissenschaftlichen Empfehlungen darstellt.

  14. Baking hot. Burger King is testing a restaurant with solar power supply; Unter der Sonne braten. Der Fastfood-Riese Burger King testet ein Restaurantkonzept mit Solaranlage

    Energy Technology Data Exchange (ETDEWEB)

    Kaizl, Mira


    At Waghaeusel in the German state of Baden-Wuerttemberg, the local solar provider Wirsol Solar AG constructed a restaurant building for the Burger King GmbH. The building consumes comparably little energy and has a solar roof for photovoltaic power generation. Burger King hopes for good money and a clean image, while Wirsol Solar is hoping for new projects in Europe and in the USA. (orig.)

  15. Energetic and ecological aspects of the modernisation of prefabricated large-panel buildings; Ergebnisse der Sanierung von industriell errichteten Gebaeuden der Blockbauart unter energetischen und oekologischen Aspekten

    Energy Technology Data Exchange (ETDEWEB)

    Schenk, H. [Gebaeudewirtschaft Merseburg GmbH (Germany); Schramek, E.R.; Kaiser, M. [Gertec GmbH, Essen (Germany); Goerres, M. [Dortmund Univ. (Germany). Lehrstuhl Technische Gebaeudeausruestung


    In the new federal states, some four million residential units are still heated with individual stoves fired with briquetted brown coal. These stoves will be replaced by modern heating systems using natural gas or fuel oil or by district heat. Action taken within the framework of this research project is to permit making informative statements as to the amount of energy to be saved by modernization measures and the potential for cutting down carbon dioxide emissions. The data provided concern space heat energy consumption, end-use energy consumption, and primary energy consumption. Regarding the pollutant budget, information on climate-relevant and other pollutants is given. (MSK) [Deutsch] In den neuen Bundeslaendern werden noch ca. 4 Millionen Wohnungen mit Braunkolebriketteinzeloefen beheizt. Diese werden durch moderne mit Erdgas und Heizoel betriebene Heizungssysteme oder Fernwaerme ersetzt werden. Die in diesem Forschungsprojekt durchgefuehrten Massnahmen sollen aussagekraeftige Daten ueber die erreichbare Energieeinsparung durch Sanierungsmassnahmen und das Minderungspotential der Kohlendioxid-Emissionen liefern. Es sind Daten zum Heizwaermeverbrauch, zum Endenergieverbrauch, sowie zum Primaerenergieverbrauch enthalten. Fuer die Schadstoffbilanz werden Angaben zu klimarelevanten und zu sonstigen Schadstoffen gemacht. (MSK)

  16. Productivity of agricultural surfaces in the eastern parts of Germany under the influence of climatic changes; Die Ertragsfaehigkeit ostdeutscher Ackerflaechen unter Klimawandel

    Energy Technology Data Exchange (ETDEWEB)

    Wechsung, Frank; Gerstengarbe, Friedrich-Wilhelm; Lasch, Petra; Luettger, Andrea (eds.)


    More than most other economic sectors agriculture depends on climatic conditions. This report presents an analysis of possible climate change effects on Eastern Germany's agriculture. It suggests that the risk of Eastern Germany's acreage losing in value is low. Negative regional impacts of climate change could be countered by appropriate adaptation measures, crop yields in many areas could even be raised and yield losses might be of set by counteracting price increases on the world market. The projection is based on a regional climate scenario. Using the statistical scenario-model STAR II available large scale projections from climate change global circulation models for middle Europe were downscaled to the existing network of East-German weather stations. The available weather records for the period 1951 to 2003 were extended for the scenario period 2004-2055. For the extension, a general increase of the average annual temperature in Eastern Germany by up to 2.7 degrees Celsius (4.8 degrees Fahrenheit) was assumed until the middle of the 21st century. The increase in temperature was triggered by a rise in atmospheric CO2 concentration from 386 at present to 542 ppm in 2055. The scenario model STAR II was used to derive the associated changes of the other major climate variables. Following the results the average annual rainfall is projected to decrease only marginally. However, shift of rainfall patterns is to be expected. Summertime rainfall will probably decrease while wintertime rainfall is likely to increase. This trend can already be monitored today and will continue as a tendency into the future. According to the projections this development will be interrupted infrequently, though, by relatively humid phases with comparatively high amounts of rainfall during the summer months. Based on this scenario, the future crop yield potentials were evaluated for the summer crop maize and the winter crop wheat in Eastern Germany. The evaluation used statistical yield models relating yearly changes in major climate variables to those in crop yield at the county level. The models were calibrated for the period 1990 to 2006 and then used for simulating the climate dominated yield variability beginning in 1951 up to 2055. The results received after integration revealed that the climate-determined yield level will probably remain stable under the projected climatic conditions for the next 20 to 30 years. A significant yield loss is to be expected by the middle of the 21st century, especially at locations in the eastern sandy lowlands distant from coast and mountain regions. The losses will be greater in the cultivation of the summer crop maize than that of the winter crop wheat. However, the increase in atmospheric concentration of carbon dioxide (CO2) might compensate part or all of these losses. Due to the fertilizing effect of this greenhouse gas, yields of wheat can even be expected to increase above current levels and yield losses of maize due to water stress will be mostly diminished. An optimum nitrogen provision is a necessary precondition for the CO2 fertilization to come to its full effect, though. In contrast to wheat and maize, yields of fast growing aspen in short rotation coppice plantation are expected to increase under the presumed changes of climate also on sandy soils with low water holding capacity even when neglecting the beneficial effect of higher CO2. This is indicated by simulation results using the eco-physiological forest dynamics model 4C. The model was applied to those areas of East-Germany's arable land where cereal cropping is less productive than the current median level. Following these results, short rotation coppicing of aspen is a promising cultivation alternative for farmers to secure future incomes. Woody biomass generated in short rotation coppice plantations with aspen is particularly suitable for bio-energy production. Taken the simulated effects of the projected climate change on crop and aspen yields and considering the global market prospects the conclusion can be drawn that the value of farmland for the production of biomass will at least remain stable in East-Germany for the next four decades. The global request for increased supply will continue to rise. Considering the given limitation for a simultaneous increase in production capacity the mean market prices will be most likely higher than those seen in the last decade. Thus, even if crop yields will decrease in some parts of Eastern Germany, monetary damages would probably be overcompensated by increases in farmers' incomes. (orig.)

  17. Entwicklung von Stallgebäuden zur Milchviehhaltung unter Berücksichtigung der klimatischen Bedingungen in ariden und semiariden Gebieten


    Georges, Marwan


    Globale Statistiken (FAO) deuten für aride und semiaride Länder auf einen Mangel bei der Milchproduktion hin. Die Unterversorgung ist nicht nur auf die geringe Milchleistung der ein-heimischen Rassen, sondern auch auf schlechte, vorhandene Stallbausysteme zurückzufüh-ren. Die Mehrzahl der Milchviehhaltungsgebäude entspricht dort nicht dem geforderten Stan-dard, vor allem im Hinblick auf ein tierangepasstes Umgebungsklima. Viele neue Material- und Konstruktionsentwicklungen zielen auf die ...

  18. Photobiological hydrogen production with the unicellular green alga Chlamydomonas reinhardtii under process engineering aspects; Photobiologische Wasserstoffproduktion mit der einzelligen Gruenalge Chlamydomonas reinhardtii unter verfahrenstechnischen Aspekten

    Energy Technology Data Exchange (ETDEWEB)

    Geier, Stephanie


    Hydrogen is of high interest as a clean and environmentally friendly energy source as its combustion only emits water and energy. However, currently hydrogen is produced in energy demanding processes by the consumption of fossil fuels. An alternative way of sustainable and non-polluting hydrogen production could be provided by use of photosynthetic active microalgae. Within this work, the photobiological hydrogen production with the unicellular green algae Chlamydomonas reinhardtii is investigated under the aspects of bioprocess-engineering and economics. Objectives are, besides the increase of the photochemical efficiency, the cultivation of the algae and subsequent hydrogen production under cost-free sunlight. It could be demonstrated that outdoor cultivation of C. reinhardtii is possible in Central Europe throughout the year by using e.g. waste heat. Similar cell numbers in the range from 1,2.10{sup 7} cells ml{sup -1} to 1,7.10{sup 7} cells ml{sup -1} could be achieved in closed photobioreactors of the type Photobioreactor Screening Module under controlled laboratory conditions and both continuous illumination (200 {mu}mol.m{sup -2}.s{sup -1}) and simulated outdoor conditions according to the light intensity of idealized summer day as well as in outdoor experiments (up to 2000 {mu}mol.m{sup -2}.s{sup -1}).The use of 10 % CO{sub 2} corresponding to the CO{sub 2} content in flue gas led to a doubling of cell numbers under continuous illumination to 4,2.10{sup 7} cells ml{sup -1}, compared to the reference culture bubbled with 3 % CO{sub 2}. A significant increase of cell numbers under the light profiles of an idealized summer day could not be achieved. The cultivation under the light profile of a winter day at 25 C reduced cell growth to 54 %, compared to the summer simulation. In open 30 L outdoor ponds, only 0,26.10{sup 7} cells ml{sup -1} could be achieved under photoheterotrophic conditions during the summer months, which corresponds to 20 % of the cell numbers produced in the closed photobioreactors. The increase of the filling height from 5 cm to 10 cm resulted in a decrease of the cell numbers by 20 % to 30 %, related to cultures with 5 cm fill height. Mixing of the ponds by pumps did not lead to an increase of cell numbers. The induction of hydrogen production as a result of sulfate deprivation was carried out through media exchange by means of centrifugation according to the state of the art. The media exchange by tangential cross-flow filtration was established successfully, but subsequent hydrogen production resulted in lower hydrogen yields. Experiments to adjust sulfate deprivation by growth related self-reduction did not lead to hydrogen evolution under the chosen parameters. Hydrogen production could be established in two photobioreactor designs and was generally higher in the simplified hydrogen photobioreactor based on a glass bottle compared to the Photobioreactor Screening Modules, which are applicable for up-scaling. The light optimum for maximum cell specific hydrogen production and hydrogen yields was determined in case of the hydrogen photobioreactor based on the Photobioreactor Screening Module to be at 100 {mu}mol.m{sup -2}.s{sup -1} with an optimal culture density of 7,5.10{sup 6} cells ml{sup -1} and in case of the bottle hydrogen photobioreactor at 200 {mu}mol.m{sup -2}.s{sup -1} with an optimal culture density of 11.10{sup 6} cells ml{sup -1}. A small variance of 12 % was observed between multiple batches of one test run with the bottle hydrogen photobioreactor, however the scattering between comparable but independent test runs was wide-ranging by 72 % despite of standardized experimental conditions. The maximum hydrogen production was achieved in the bottle hydrogen photobioreactor with 130 ml L{sup -1} H{sub 2} under continuous light from one side with 200 {mu}mol.m{sup -2}.s{sup -1} over a period of 100 hours (incl. adaption). The space-time yield amounted to 32 ml H{sub 2}.L{sup -1}.d{sup -1}. Furthermore, it could be demonstrated that cells from running hydrogen production could be regenerated and achieved the usual hydrogen amount in the second hydrogen production run. The use of algae cells grown under outdoor, simulated outdoor, or strong light conditions respectively resulted in significantly lower hydrogen yields compared to the use of cells cultivated under continuous illumination and lower irradiance. The maximum hydrogen yield of cells under outdoor or simulated outdoor conditions respectively accounted for 8 ml L{sup -1} H{sub 2}. Due to inhibition of hydrogen production in the scalable Hydrogen Photobioreactor Screening Module at light intensities of 300 {mu}mol.m{sup -2}.s{sup -1}, experiments under day light profiles with light intensities up to 2000 {mu}mol.m{sup -2}.s{sup -1} or outdoor experiments respectively were desisted. In order to increase the photochemical efficiency of C. reinhardtii, the overexpression of hydrogenase was tested. Despite the successful integration of the additional species-specific hydrogenase gene into the genome, the recombinant protein could not be detected by immunostaining and no significant increase of hydrogen production could be measured. (orig.)

  19. Improved load flow control for distribution system operators under consideration of wind and solar performance prognosis; Verbesserte Lastflussfuehrung fuer Verteilnetzbetreiber unter Einbeziehung von Wind- und Solarleistungsprognosen

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, Ralf [IDS GmbH, Ettlingen (Germany); Strunk, Achim [EWC Weather Consult GmbH, Karlsruhe (Germany)


    Smart grid and smart metering are the results from the social and legal intention to detach from the nuclear energy, to expand the production of renewable energy and to bring more competition into the energy market. The next decade will be characterized by extensive investments to integrate the myriad of distributed production plants into the network management, optimal use of the networks and the offer of flexible tariffs to end-users in order to respond to the effects of the weather-related production potential. With the predictability of depression measures distribution operators would be able to optimize the purchase from suppliers, the production in its own network and the feed-in performance of renewable energy systems and to derive the economic benefit from this. This requires a holistic approach to forecast which surpasses far beyond previous approaches for the optimization of the energy supply based on load profiles and forecasts of temperature and wind.

  20. Beißvorfälle unter Berücksichtigung der Hunderassen in Deutschland und Umfrage bei Hundebisspatienten in vier Berliner Kliniken


    Roiner, Kathrin


    In the past years, all federal states within Germany have passed laws and regulations on dog keeping. These laws and regulations are e.g. lead and muzzle compulsion at all times, some even forbid reproduction. Also, the dog owners often have to pay higher fees for the dog licenses or have to obey other rules and regulations. Almost all of these laws are solely categorizing restricted dogs based on their breed resulting in a bad reputation for these listed breeds, fear and less adoptions of sh...

  1. Determination of W-erosion through optical spectroscopy under ITER-relevant plasma conditions; Bestimmung der Wolframerosion mittels optischer Spektroskopie unter ITER-relevanten Plasmabedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Laengner, Marko


    Tungsten (W) is used in present fusion experiments and is as well a material choice for future reactors like ITER and DEMO. However, tungsten eroded from the wall leads to high radiation losses in the plasma and the transport to the plasma center to critical plasma cooling. Therefore a detailed investigation of the interaction between eroded tungsten and the plasma is necessary. The emission of tungsten line radiation in the plasma and thus the shape of the emission profiles is characterized by excitation and ionisation. Quantitatively the ionisation can be described in terms of the ionisation rate coefficients. This knowledge is necessary for the interpretation of spectroscopic data and especially to determine tungsten fluxes into the plasma that are caused by tungsten erosion. Up to now, ionisation rate coefficients for neutral tungsten were not determined except for the plasma temperature range below 20 eV. For higher temperatures only uncertain calculated data or semi-empirical data is available. Therefore, within this thesis for the first time ionisation and emission processes of tungsten originating from tungsten erosion were investigated in the temperature range between (41±8) eV and (81±8) eV at the tokamak TEXTOR and ionisation rate coefficients were determined experimentally. Measured emission profiles of the W I(400.88 nm)-line show a typical exponential decrease that is caused by the ionisation of the neutral tungsten atoms. But moreover, at a distance of 1 to 2 mm away from the sputtered surface the emission initially increases to a maximum. By introducing a relaxation time between 0.5 and 1 μs before reaching the equilibrium of the population of the emitting energy level of this line the increase of the emission profiles can be reproduced. Simulating the emissions profiles through a Monte-Carlo-simulation that was developed in the scope of this thesis results in ionisation rate coefficients that are identical with values calculated through using the semi-empirical Lotz-formula. The necessary extension of the atomic data base regarding neutral tungsten leads to the interest in a tungsten source that can easily be used for calibration purposes. This was realised at TEXTOR through the injection of WF{sub 6}. The tungsten that was brought into the plasma via WF{sub 6}-injection was investigated regarding the comparability to tungsten originating from a sputtering process. Moreover was WF{sub 6} used for the first time to determine conversion factors - the so-called photon efficiencies - to calculate tungsten fluxes from spectroscopically measured photon fluxes. For the given combinations of temperature and density from (20±5) eV to (82±5) eV and from (2.2±1) x 10{sup 12} cm{sup -3} to (6.8±1) x 10{sup 12} cm{sup -3}, respectively, for the W I(400.88 nm)-line a constant average value of left angle E{sup WI(400.88} {sup nm)}{sub Ph} right angle =94±16 is found. To test these values sputtering yields were determined from the amount of eroded tungsten atoms. These sputtering yields are in line with experimental reference values and calculations. The prevailing investigated plasma conditions are in the range of what can be expected considering the ITER divertor during ELMs. The found experimental results are consistent and confirm the match between WF{sub 6} photon efficiencies and photon efficiencies of sputtered tungsten.

  2. Untersuchungen zum Vorkommen von Myxobakterien in von Meerwasser beeinflußten Substraten unter besonderer Berücksichtigung der Insel Helgoland (United States)

    Rückert, G.


    Representatives of the family Myxococcaceae, Myxococcus fulvus and M. virescens as well as Archangium gephyra could be isolated from marine sediments (depth range 5 58 m), collected near the island of Helgoland (North Sea); dunes and rudiments of salt marshes additionally yielded M. coralloides and the rare species Melittangium licenicola and M. boletus (Cystobacteriaceae). In soil samples from the island, M. fulvus, M. virescens, M. coralloides, A. gephyra, Cystobacter fuscus and Stigmatella erecta were found. These results were confirmed by data, obtained from the coastal zone of the island of Amrum and marine sediments from various regions. On the other hand samples from shallow fresh water (depth range 0.3 1 m) proved to be richer in species. It is assumed that the myxobacteria found in marine sediments occur as resting cells.

  3. Automation of innovative facade systems with integrated technical building equipment under consideration of comfort aspects; Automatisierung innovativer Fassadensysteme mit integrierter technischer Gebaeudeausruestung unter Beruecksichtigung von Behaglichkeitsaspekten

    Energy Technology Data Exchange (ETDEWEB)

    Hasert, Anita; Becker, Martin [Hochschule Biberach (Germany). Inst. fuer Gebaeude- und Energiesysteme (IGE)


    Facades are not only a shell of the protected habitats and a boundary between the indoor climate and the environment of buildings. Facades also convert from previously passive elements to active building systems which perform various functions of the room conditioning (heating, cooling, ventilation, lighting, and so on). The associated increased demands on the system integration into facades require new solutions for the planning, implementation and operation of these innovative systems. Within the intelligent handling of increasing complexity, superior automation strategies have to be developed by means of facade automation. These automation strategies have to match the individual functions with each other, and to ensure a building sector comprehensive functionality. Furthermore, another criterion for the design of integrated facade systems is the consideration of the user's feeling with respect to comfort as well as the user's control and user's acceptance. In line with the research project AUTiFAS (= Automation of innovative facade systems), different automation strategies of the facades and room automation are considered on the basis of metrological investigations and simulation analyses. For this purpose, an innovative facade element with a decentralized ventilation unit and an integrated sunshade had to be integrated into a test room initially. The functionality and the constructional tightness of the total test stand had to be verified and matched to the requirements of the tests. With the objective to develop a standardized description of the control and regulation functions of the building sector comprehensive automation strategies, an automation library was developed based on standard structures and forms of representation using a test facade as an example. The standards DIN EN 15232, IEC 61131 as well as the guidelines VDI 3813 and VDI 3814 provide the fundamentals. The developed automation strategies form the basis for the development of new control strategies of high quality. Two approaches of highly qualitative control methods were examined in this research project. The picking up of comfort criteria in the optimized control enables the consideration of user's needs. Another approach is the predictive control which is based on characteristic line models and aims a more energy-efficient operation.

  4. Anatomy of the fingers in MR imaging using a high resolution surface coil; Die Anatomie der Finger in der MRT unter Anwendung einer Hochaufloesungsspule

    Energy Technology Data Exchange (ETDEWEB)

    Maeurer, J. [Strahlenklinik und Poliklinik, Klinikum Rudolf Virchow, Standort Wedding, Freie Univ. Berlin (Germany); Mueller, H.F. [Strahlenklinik und Poliklinik, Klinikum Rudolf Virchow, Standort Wedding, Freie Univ. Berlin (Germany); Lemke, A.J. [Strahlenklinik und Poliklinik, Klinikum Rudolf Virchow, Standort Wedding, Freie Univ. Berlin (Germany); Requardt, R. [Siemens AG, Erlangen (Germany); Stein, I. [Strahlenklinik und Poliklinik, Klinikum Rudolf Virchow, Standort Wedding, Freie Univ. Berlin (Germany); Schedel, H. [Strahlenklinik und Poliklinik, Klinikum Rudolf Virchow, Standort Wedding, Freie Univ. Berlin (Germany); Langer, R. [Strahlenklinik und Poliklinik, Klinikum Rudolf Virchow, Standort Wedding, Freie Univ. Berlin (Germany); Felix, R. [Strahlenklinik und Poliklinik, Klinikum Rudolf Virchow, Standort Wedding, Freie Univ. Berlin (Germany)


    MR-images of the fingers of twenty healthy volunteers were obtained at a 1,5-T whole-body system with a high resolution coil. Additionally, the fingers of two formalin-fixed anatomical specismen were imaged, fronzen and then subsequently sectioned for anatomic correlation. Measurements were recorded with a slice thickness of 2 mm. A 256x256 matrix and a 2,5 cam fiel of view (FOV) were used resulting in a pixel size of 0,01 mm{sup 2}. Based on the excellent depiction of anatomic detail and contrast resolution high resolution MRI of the fingers enabled identification of osseous and cartilaginous structures, as well as delineation of tendons, ligaments blood-vessels and dermal layers. (orig.) [Deutsch] An einem 1,5-T-Ganzkoerpertomographen wurden die Finger von 20 gesunden Probanden mit einer Hochaufloesungsspule zur Darstellung der Anatomie untersucht. Zum Vergleich erfolgte die Abbildung der anatomischen Strukturen an zwei formol- und alkoholfixierten Leichepraeparaten der Hand mittels MRT. Im Anschluss wurden die untersuchten Regionen am tiefgefrorenen Praeparat mit einer Bandsaege nachgeschnitten. Die MR-Messungen wurden mit einer Schichtdicke von 3 mm, einem Field of View (FOV) von 25 mm und einer Matrix von 256x256 Pixel durchgefuehrt. Die daraus resultierende Pixelgroesse betrug 0,01 mm{sup 2}. Der Weichteilkontrast und die hohe Ortsaufloesung ermoeglichten die Identifikation von Knochen- und Knorpelstrukturen sowie die Abgrenzung von Sehnen, Baendern, Blutgefaessen und Hautschichten. (orig.)

  5. Ammonia from manure slurry and measures for its reduction - in particular in connection with fermentation; Ammoniakemissionen aus Guelle und deren Minderungsmassnahmen unter besonderer Beruecksichtigung der Vergaerung

    Energy Technology Data Exchange (ETDEWEB)

    Hersener, J.-L.; Meier, U.; Dinkel, F.


    This report for the Swiss Federal Office of Energy (SFOE) presents the results of a study made to demonstrate the potential of reducing ammonia emissions from manure slurries in connection with anaerobic digestion. The various methods of treatment that were assessed, including digestion, aeration, separation of the liquid phase, additives, acidification, flocculation, precipitation and stripping are discussed. The findings on the effectiveness of the different treatment methods are compared with normal slurry storage and distribution methods (open pits for storage, distribution by splash plate). Recommendations on convenient methods for reducing ammonia losses are made, whereby the authors state their opinion that digestion is not to be recommended and that separation is practical and can be realised rapidly.

  6. Climatic change and the effects on the marine ecosystem around the island of Sylt. Final report; Das marine Oekosystem um Sylt unter veraenderten Klimabedingungen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Lackschewitz, D.; Menn, I.; Reise, K.


    Climatic warming of 1.5 to 2.5 C with a higher water temperature may only slightly change the species spectrum of the intertidal zone of the island of Sylt (North Sea). This is shown by a comparison with tidal flats of the French Atlantic coast. Stronger effects are expected from a sea level rise and an increase in hydrodynamic forces. This may result in a decrease of eelgrass and mussel beds. On the high intertidal zone muddy flats will be replaced by sandy flats due to wave action. Enhanced erosion at the Wadden Sea shoreline will probably entail its continued petrification to prevent further losses. This will cause a decrease of natural habitats with their specific assemblages, and the esthetic appeal of the Wadden Sea will decrease too. It is proposed that sand replenishment on the Wadden Sea shoreline will better preserve the natural sequence of biotopes on the tidal flats. The erosive beach on the seaward side of the island of Sylt is both focal place for tourist recreation, and the site of a highly diverse interstitial fauna. This fauna will be able to re-establish itself three months after a campaign of sand replenishment. Sand replenishment was found to be an effective way to compensate beach erosion and, due to the quick re-establishment of the fauna, it may be regarded as an acceptable method of coastal defense from an ecological perspective. (orig.) [German] Bei einer Klimaerwaermung um 1,5 bis 2,5 C wird der direkte Einfluss hoeherer Wassertemperatur das biologische Artenspektrum im Sylter Gezeitenbereich nicht wesentlich veraendern. Dies ergibt ein Vergleich mit Watten der franzoesischen Atlantikkueste. Bedeutender sind voraussichtlich Auswirkungen hoeherer Wasserstaende und einer zunehmenden Hydrodynamik. Seegraswiesen und Muschelbaenke werden dadurch im Wattbereich abnehmen. In Ufernaehe werden schlickige von sandigen Watten verdraengt. Einer verstaerkten Erosion an ungeschuetzten Wattufern wird voraussichtlich mit weiteren Befestigungen begegnet. Dadurch werden natuerliche Uferbiotope mit ihren charakteristischen Lebensgemeinschaften auf der Syler Wattseite selten. Bei solchen Veraenderungen nimmt der Erlebniswert der Watten ab und es leidet die Landschaftsaesthetik. Dem koennte durch kuenstliche Sedimentversorgung auf der Wattseite von Sylt entgegengewirkt werden. Der fuer die Erholung attraktive Sylter Brandungsstrand ist durch eine aeusserst vielfaeltige Fauna des Sandlueckengefueges gekennzeichnet. Sie kann als Indikator fuer eine hohe Qualitaet des Badestrandes gelten. Nach Sandvorspuelungen zum Ausgleich von Erosionsverlusten stellt sich diese Fauna schon nach drei Monaten weitgehend unveraendert wieder ein. Die partielle Umwandlung von Erosionsstraenden in Depositionsstraende durch eventuelle Baumassnahmen des Kuestenschutzes wuerde die Biodiversitaet der Sandlueckenfauna verringern. (orig.)

  7. Characterization of polyelectrolytes and lithium salts for electrochemical energy storage devices using novel measurement systems; Charakterisierung von Polyelektrolyten und Lithiumsalzen fuer elektrochemische Energiespeicher unter Verwendung neu entwickelter Messsysteme

    Energy Technology Data Exchange (ETDEWEB)

    Huber, Benedikt


    In the first part of this work, three imidazolium-based ionic liquid monomers with polymerizable vinyl groups and the resulting polyelectrolytes have been synthesized and characterized. Particular attention was paid to the purity of the materials. Besides comprehensive monomer and polymer analytics, electrical impedance spectroscopy was carried out to obtain information about the ion conducting properties of the three systems under investigation: poly(3-ethyl-1-vinylimidazolium)-bis(trifluoromethanesulfonyl)imide (P1), poly(3-methyl-1-(4-vinylbenzyl)imidazolium)-bis(trifluoromethanesulfonyl)imide (P2) and poly(1-butyl-3-methyl-2-(4-vinylphenethyl)imidazolium)-bis(trifluoromethanesulfonyl)imide (P3). The pure polymers, which are bis(trifluoromethanesulfonyl)imide (N(Tf)2) anion conductors, exhibit room-temperature conductivities of the order of 10-8 S/cm in the best case. The anion conduction mechanism is strongly influenced by the length of the spacer group between the polymer backbone and the imidazolium cations attached to the side chain. In polymers P1 and P2 with short spacer groups, intra- and inter-cation hopping of the N(Tf)2 anions can be distinguished below the glass transition temperature, while this is not possible in the case of polymer P3 with longer spacer groups. Furthermore, we have studied several mixtures of the best conducting polymer P2 with LiN(Tf)2, zwitterions and monomeric ionic liquid. While the zwitterions were capable of compensating for the conductivity drop due to Li salt addition, the addition of monomeric IL as plasticizer leads to a considerable conductivity enhancement without a significant loss of mechanical stability. In the second part of this work, three lithium salts, lithium bis(pentafluorophenyl)amide LiN(Pfp)2, lithium pentafluorophenyl-trifluoromethyl-sulfonylimide LiN(Pfp)(Tf) and lithium pentafluorophenyl-nonafluorobutyl-sulfonylimide LiN(Pfp)(Nf) were characterized with respect to their thermal and electrochemical properties. LiN(Pfp)2 decomposes around 100 C, whereas LiN(Pfp)(Tf) and LiN(Pfp)(Nf) show a much higher thermal stability up to temperatures above 300 C. The ionic conductivity at 100 C, measured by means of impedance spectroscopy, decreases in the order LiN(Pfp)(Tf) > LiN(Tf)2 > LiN(Pfp)(Nf). Both, the activation energy and entropy for ion conduction in the new salts are lower than in LiN(Tf)2, most likely due to the lower symmetry of the new anions. The electrochemical stability and ionic conductivity of LiN(Pfp)(Tf) and LiN(Pfp)(Nf) solutions in alkyl carbonates are slightly lower than that of the LiN(Tf)2 solution, but still sufficient for application in lithium ion batteries.

  8. Requirement-specific use of trace element biogas additives under consideration of the bioavailability; Bedarfsgerechter Einsatz von Spurenelement-Biogasadditiven unter Beruecksichtigung der Bioverfuegbarkeit

    Energy Technology Data Exchange (ETDEWEB)

    Feher, Adam; Fritz, Thomas; Loewen, Achim; Nelles, Michael [HAWK Hochschule fuer Angewandte Wissenschaft und Kunst Hildesheim/Holzminden/Goettingen, Goettingen (Germany). Fachgebiet Nachhaltige Energie- und Umwelttechnik (NEUTec)


    A highly efficient utilisation of substrates and operational stability at the same time is essential for the efficiency of a biogas plant. The research project SequEx of HAWK and /SF GmbH investigates the increase of efficiency of biogas plants by targeted use of trace elements taking into account bio-availability. Based on sequential extraction diagrams a method for the determination of bio-availability of macro- and micro-nutrients is developed. Within this context, different trace element compounds are analysed during the fermentation process on their share of bioavailable micro-nutrients as well as their effect on the biological activity of microorganisms. The actual knowledge gap between common practice of nutrients' supply and real impact shall be closed by the knowledge base enhancement of the bio-availability of nutrients. (orig.)

  9. Partitioning and transmutation of transuranium elements under nuclear phase-out conditions. Technically reliable?; Transmutation von Transuranen unter den Randbedingungen des Kernenergieausstiegs. Technisch machbar?

    Energy Technology Data Exchange (ETDEWEB)

    Merk, Bruno; Rohde, Ulrich [Helmholtz-Zentrum Dresden-Rossendorf, Dresden (Germany)


    The German government has decided for the nuclear phase out, but a decision on a strategy for the management of the highly radioactive waste is not defined yet. Partitioning and Transmutation (P and T) could be considered as a technological option in the process of management of highly radioactive waste management, therefore a wide study has been conducted. In this group objectives for P and T and the boundary conditions of the phase out have been discussed. The fulfillment of the given objectives is analyzed using simulations of molten salt reactors with fast neutron spectrum. It is shown that the efficient transmutation of all existing transuranium isotopes would be possible in 3 to 4 reactors in a time frame of 45 to 60 years. Further on a detailed balance of different isotopic inventories is given to allow a deeper understanding of the processes during transmutation.

  10. Local energy supply under national and European law. With special regard to municipal policy opportunities. Oertliche Energieversorgung nach nationalem und europaeischem Recht. Unter besonderer Beruecksichtigung kommunaler Gestaltungsmoeglichkeiten

    Energy Technology Data Exchange (ETDEWEB)

    Britz, G.


    Conceivably, the erection of a single European energy market for electricity and natural gas as specified in the EC draft guidelines may change the conditions of local energy supply. This thesis therefore investigates which instruments are at the disposal of municipal governments for the realization of energy-political concepts of their own: Rights of way and granting of franchises, establishment of and transfer of tasks to municipal utilities, common carvier duties, and free choice of suppliers by distributors. The handling of franchise payments and treatment of municipal interconnected networks are of considerable importance for the financial situation of communities. The first section deals with the legal issues of local energy supply with regard to national law. The second part deals with the same questions with regard to community law. Furthermore it is considered what would be the consequences of the realization of the two guidelines concerning the single energy market. In the final section the results are compared and the significance of community law for local energy supply is assessed. (orig./HP)

  11. Molekularbiologische Analyse der genetischen Diversität des Melitaea athalia / celadussa-Komplexes (Lepidoptera: Nymphalidae) unter Anwendung der ISSR-PCR auf Art-, Unterart- und Populationsebene


    Achtelik, Gerdo


    Mit Hilfe der ISSR-PCR wurde erstmalig eine großräumige Populationsanalyse bei einer Tagfalterart am Beispiel von Melitaea athalia vorgenommen. Es konnten fünf verwandte Arten der Nymphalidae - Melitaea diamina, M. cinxia und M. aurelia sowie Boloria euphrosyne und Issoria lathonia - untereinander sowie gegenüber M. athalia differenziert werden. 90 Primer wurden geprüft, 8 Primer amplifizierten allein für M. athalia 410 Marker. Bei allen Taxa wurde eine sehr hohe Polymorphismusrat...

  12. Evaluation einer elektronisch unterstützten pflegerischen Überleitung zwischen Krankenhaus und Pflegeheim unter Nutzung einer Test-Telematikinfrastruktur: eine Fallanalyse

    Directory of Open Access Journals (Sweden)

    Schulte, Georg


    Full Text Available Background: Improper information transmission can lead to compromised patient safety and quality of life when patients are transferred from one setting to another. Electronic instruments may improve this situation, however, they are rarely used. Objective: The aim of this study therefore was to investigate the technical and organizational feasibility, usability, usefulness and completeness of an electronic instrument that is based on the German HL7 CDA standard for eNursing Summaries.Materials and methods: To this end, a test health telematics infrastructure, which included the German electronic health card, was established and nursing summary application was developed that allowed summary documents to be communicated between a hospital and a nursing home. The users were asked to evaluate the usability of the nursing summary application as well as to compare the usefulness and completeness of electronically and paper transmitted information.Results: This study demonstrated the feasibility of implementing an electronic nursing summary application that was based on the German HL7 CDA standard eNursing Summary and that was integrated in a test health telematics infrastructure. It could also be shown that the users rated this application as usable and that electronically supported patient transfers were superior to paper based ones. The use of the German electronic health card was regarded as a barrier by the users.Discussion: This study emphasizes the feasibility, relevance and barriers of electronically supported transfers of patients with nursing needs. Nurses working in hospitals and long-term care can integrate an application based on the HL7 CDA Standard ePfgebericht into their working processes and get better and more complete information. To ensure continuity of care in a sustainable manner in the future, the German HL7 CDA based eNursing Summary standard should become part of the German telematics infrastructure.

  13. Experimental investigations on the coolability of prototypical particle beds with respect to reactor safety; Experimentelle Untersuchungen der Kuehlbarkeit prototypischer Schuettungskonfigurationen unter dem Aspekt der Reaktorsicherheit

    Energy Technology Data Exchange (ETDEWEB)

    Leininger, Simon


    In case of a severe accident in a light water reactor, continuous unavailability of cooling water to the reactor core may result in overheating of the fuel elements and finally the loss of core integrity. Under such conditions, a structure of heat-releasing particles of different size and shape may be formed by fragmentation of molten core material in several stages of the accident. The long-term coolability of such beds is of prime im-portance to avoid any damage to the reactor pressure vessel or even a release of fission products to the environment. In the frame of this work, specific experiments were con-ducted under prototypical conditions employing the existing DEBRIS test facility in order to gain further knowledge about the thermohydraulic behavior of such beds. In steady state boiling experiments, the pressure gradients in particle beds were meas-ured both for one- and multi-dimensional cooling water flow conditions and compared with one another in order to assess the flow behavior inside the bed. For these different flow conditions as well as for stratified bed configurations, the maximum removable heat flux densities were determined in the dryout experiments. E. g., it was found that an axial stratification of the permeability can significantly reduce the bed's coolability. For the first time, the quenching behavior of dry, superheated beds was investigated at elevated system pressure up to 0.5 MPa. In these experiments, the effect of system pressure on the coolability was quantified by means of the quenching time (time period to cool down the bed to saturation temperature). The investigated particle beds mainly consisted of non-spherical particles with well-defined geometry (cylinders and screws). It was shown that the effect of the particles geometry on the flow in a particle bed can be best estimated by using an equivalent particle diameter calculated for monodisperse particle beds from the product of the Sauter diameter and a shape factor and for polydisperse beds from the surface-averaged diameter. Accompanying exemplary model calculations have confirmed the applicability of the MEWA code for assessment of the coolability of both water-saturated and dry beds. Nevertheless, the implemented friction models should be improved to better predict the pressure gradient of two-phase flows in particle beds.

  14. Material flows of benzene with particular consideration of the Federal Republic of Germany; Stoffstroeme von Benzol unter besonderer Beruecksichtigung der Bundesrepublik Deutschland

    Energy Technology Data Exchange (ETDEWEB)

    Boehncke, A.; Mangelsdorf, I. [Fraunhofer Inst. fuer Toxikologie und Aerosolforschung, AG Dokumentation und Bewertung von Chemikalien, Hannover (Germany); Rosner, G. [ToxConsult, Merzhausen (Germany)


    In the Federal Republic of Germany, benzene is one of the most important basic materials for the chemical industry. Only a relatively small proportion of the pure benzene processed in the chemical industry is emitted into the environment (ca. 40 t in 1991). But the substance is also a natural component of the crude oil in gasoline and is released during incomplete combustion or is formed out of other aromatic substances. The primary source of emissions, with more than 10,000 t/yr (approximately 85% of this from motor vehicles with Otto engines), is commercial motor vehicle transportation. Benzene concentrations in the environment are approximately <1 {mu}g/m{sup 3} in rural areas, 20-30 {mu}g/m{sup 3} near main roads (peak levels in highly urbanized regions with much traffic as high as approximately 100 {mu}g/m{sup 3}) and 7-15 {mu}g/m{sup 3} in the vicinity of industrial polluters. It has not been possible to detect a specific trend over time during the last ten years. An increased exposure (approx. 350-27,000 {mu}g/m{sup 3}) is likely while filling the tank and within the motor vehicle (approx. 10-200 {mu}g/m{sup 3}) due to gasoline volatilization from pipes, etc. Compared to outdoor air, higher concentrations of benzene (approx. 2-11 {mu}g/m{sup 3}) are measured in the indoor air which contains additionally benzene from tobacco smoke, equipment, renovating work and heating. The primary exposure pathway of benzene in humans is inhalation. Apart from individuals with occupational exposure, smokers have the highest internal benzene burden. Measures undertaken during recent years to reduce the amount of emissions have been counteracted at least in part by the increase in motor vehicle traffic. Further measures to reduce the emissions or to change the transportation policies must still be or have already been initiated. (orig.) [Deutsch] Benzol stellt fuer die chemische Industrie in der Bundesrepublik Deutschland einen der wichtigsten Grundstoffe dar. Das in der chemischen Industrie verarbeitete Reinbenzol gelangt nur zu einem relativ geringen Teil in die Umwelt (1991 ca. 40 t). Die Substanz ist ausserdem als natuerlicher Bestandteil des Rohoels in Vergaserkraftstoffen enthalten und wird bei deren unvollstaendiger Verbrennung freigesetzt bzw. aus anderen Aromaten neu gebildet. Die Haauptquelle der Emissionen stellt mit mehreren 10.000 t/a (davon ca. 85% aus Ottomotoren/Kfz) der Kraftfahrzeugverkehr dar. Die Immissionskonzentrationen liegen im allgemeinen bei <1 {mu}g/m{sup 3} fuer laendliche Gebiete, 20-30 {mu}g/m{sup 3} an Hauptverkehrsstrassen (Spitzenwerte in verkehrsreichen Ballungsgebieten bis zu ca. 100 {mu}g/m{sup 3}) und 7-15 {mu}g/m{sup 3} in der Naehe industrieller Emittenten. Fuer die letzten zehn Jahre konnte aus den vorliegenden Daten kein zeitlicher Trend abgeleitet werden. Erhoehte Expositionen sind insbesondere waehrend des Tankvorgangs (ca. 350-27.000 {mu}g/m{sup 3}) und bei Ausduenstungen aus kraftstoffuehrenden Teilen in Kfz-Innenraeumen (ca. 10-200 {mu}g/m{sup 3}) moeglich. In Raumluft wurden gegenueber der Aussenluft erhoehte Benzolkonzentrationen gemessen (ca. 2-11 {mu}g/m{sup 3}), die neben den Aussenlufteinfluessen auf Tabakrauch, Ausstattungsmaterialien, Renovierungstaetigkeiten und Heizungen zurueckzufuehren sind. Die inhalative Aufnahme von Benzol ist der wesentliche Belastungspfad des Menschen. Raucher weisen neben beruflich exponierten Personen die hoechste innere Belastung auf. Die in den letzten Jahren eingefuehrten emissionsmindernden Massnahmen wurden im Bereich des Kfz-Verkehrs zumindest zum Teil durch ein gestiegenes Verkehrsaufkommen kompensiert. Weitere Emissionsminderungs- und verkehrspolitische Massnahmen sind anzustreben oder wurden bereits initiiert. (orig.)

  15. Reduction of greenhouse gas emissions in the rape cultivation with special consideration of nitrogen fertilization; Minderung von Treibhausgasemissionen im Rapsanbau unter besonderer Beruecksichtigung der Stickstoffduengung

    Energy Technology Data Exchange (ETDEWEB)

    Heilmann, Hubert [Landesforschungsanstalt fuer Landwirtschaft und Fischerei Mecklenburg-Vorpommern, Guelzow-Pruezen (Germany). Inst. fuer Pflanzenproduktion und Betriebswirtschaft; Riemer, Doerte


    Involved into the research project ''Mitigation of greenhouse gas emissions in oilseed rape cropping with special consideration of nitrogen fertilizing'' regional specific GHG cropping emissions according to benchmark and regional experts are calculated by using a calculation method developed in cooperation with IFEU and according to IPCC (2006). The following results are achieved for 35 German NUTS2-regions: - nitrogen fertilization is the main influence for GHG emission reduction; - the use of low-emission nitrogen fertilizers is worth for GHG emission reduction; - without increasing the nutrient efficiency of organic fertilizers, GHG emission reductions are difficult to achieve in many regions; - GHG emission reduction/climate protection and realization of the WRRL or N-Saldo reduction come up to the same aim; - economic consequences of restrictive carbon mitigation can be compensated by slight price surcharges for certified raw material.

  16. Approaches to the dimensioning of enhanced biological phosphorus elimination systems, taking dynamic simulation into account; Bemessungshinweise zur vermehrten biologischen Phosphorelimination unter Beruecksichtigung der dynamischen Simulation

    Energy Technology Data Exchange (ETDEWEB)

    Scheer, H


    With so many projects either planned or under construction, the question of the dimensioning of sewage treatment plants with enhanced biological phosphorus elimination (BIO-P) is becoming more and more important. A detailed search of literature established in how far dimensioning approaches or models were already available in the spring of 1994. These modelling approaches were critically examined and compared as to their practical applicability by means of parameter and sensitivity studies. For this purpose, they were programmed and the relevance of certain dimensioning parameters to biological phosphorus elimination was studied by means of a pilot plant. (orig./SR) [Deutsch] Der Auslegung von Klaeranlagen mit vermehrter biologischer Phosphorelimination (BIO-P) kommt bei der Vielzahl von Planungs- und Baumassnahmen eine immer wichtigere Bedeutung zu. Inwieweit fuer die Bemessung von Klaeranlagen mit BIO-P im Fruehjahr 1994 bereits auf vorhandene Bemessungsansaetze und -modelle zurueckgegriffen werden konnte, wurde mittels einer detaillierten Literaturstudie, untersucht. Diese Modellansaetze wurden im Hinblick auf ihre praxisorietierte Anwendbarkeit durch Parameter- und Sensitivitaetsstudien kritisch untersucht und verglichen. Hierzu wurden die verschiedenen, zum damaligen Zeitpunkt vorhandenen Ansaetze programmiert und die Auswirkungen wichtiger bemessungsrelevanter Parameter auf die BIO-P anhand einer Modellklaeranlage abgeschaetzt. (orig./SR)

  17. No need for an electric battery. Solar gadgets for walks and hikes; Staendig unter Strom. Solargeraete fuer kleine und grosse Wanderungen

    Energy Technology Data Exchange (ETDEWEB)

    Siemer, Jochen


    While it is just a minor nuisance when a MP3 player breaks down during a short sunday's cycle tour, it may be more of a problem when a lamp or GPS cell phone does not have sufficient electricity supply on a mountain hike of several days. The contribution presents a number of commercially available solar emergency power supplies. While some of these are no more than amusing, others are power supplies to be reckoned with. (orig.)

  18. Participation in the radioactive waste disposal procedure under consideration of the site selection law; Partizipation bei der Entsorgung radioaktiver Reststoffe - unter besonderer Beruecksichtigung des Standortauswahlgesetzes

    Energy Technology Data Exchange (ETDEWEB)

    Smeddinck, Ulrich; Rossegger, Ulf [TU Braunschweig (Germany). Inst. fuer Rechtswissenschaften


    The site selection law has passed the parliament. The history and the content of the law indicate the difficulties of an adequate public participation. The contribution describes and analyzes the relevant and interdisciplinary facets of the issue. The contribution is focused on the legal regulations concerning the participation trying a classification into higher ranking relations. For a final evaluation the further development has to be considered.

  19. Post-mortal changes in blood Cd levels in consideration of Cd levels in organs. Postmortale Veraenderung der Cadmiumkonzentration im Blut unter Beruecksichtigung der Organcadmiumkonzentrationen

    Energy Technology Data Exchange (ETDEWEB)

    Goetz, D.


    After a detailed introduction, in which the properties and occurrence of Cd, the Cd metabolism and the Cd concentration in blood and organs, and the effects of smoking are discussed, the author presents the results of Cd concentration measurements in the biological materials of a collective of patients who died in the intensive care unit of an internal ward. In all cases, the postmortal Cd concentration in venal blood was much higher than the in-vivo concentration. The concentration increase is not noticeable in the first two hours post mortem; from the forensic view, it is therefore recommended to sample blood as early as possible in case of suspected Cd poisoning. After a latency period of 38 to 92 h, intracardial blood Cd concentrations will be high enough to suspect Cd intoxication, or at least a very high Cd exposure. This tentative diagnosis of Cd intoxication can be proved or disproved only by the rather tedions measurement of Cd concentrations in organs. Postmortal toxicological blood screening should, therefore, not lead to hasty conclusions. The contribution of smoking to Cd concentrations was clearly proved by comparisons of blood Cd concentrations and organ Cd concentrations of smokers and nonsmokers.

  20. Long-term behaviour of concrete under saline conditions for long-term stable sealing structures; Langzeitverhalten von Beton unter salinaren Bedingungen fuer langzeitstabile Verschlussbauwerke

    Energy Technology Data Exchange (ETDEWEB)

    Dahlhaus, Frank; Haucke, Joerg [Technische Univ. Bergakademie Freiberg (Germany). Inst. fuer Bergbau und Spezialtiefbau


    The authors of the contribution under consideration examine the long-term behaviour of concrete under saline conditions and in particular the suitability of the dam construction materials salt concrete and brine concrete for the use as a part of a sealing system of long-term stable geotechnical sealing structures. The long-term stability of the building material mainly is determined by the corrosion of the cement paste phases. The specific shrinkage behaviour of the construction material is analyzed experimentally in order to verify the expected cracks. The mechanisms of cracking in the salt concrete and brine concrete are analyzed by means of a mesomechanical approach in numerical finite-element calculations.

  1. Room temperature fatigue behaviour of a normalized steel SAE 4140 in torsion. Ermuedungsverhalten von normalisiertem 42CrMo4 unter Torsionsbeanspruchung bei Raumtemperatur

    Energy Technology Data Exchange (ETDEWEB)

    Klumpp, S.; Eifler, D.; Macherauch, E. (Karlsruhe Univ. (T.H.) (Germany, F.R.). Inst. fuer Werkstoffkunde 1)


    Cyclic deformation behaviour of a normalized steel SAE 4140 in shear strain-controlled torsion is characterized by cyclic softening and cyclic hardening. If mean shear stresses are superimposed to an alternating shear stress, cycle-dependent creep occurs, and the number of cycles to failure decreases. In shear strain-controlled torsional loading, mean stresses are observed to relax nearly to zero within a few cycles. Fatigue life is not influenced by mean shear strains. (orig.).

  2. Lebensdauervorhersage fur partikelverstaerkte Aluminiumlegierungen unter Beruecksichtigung der mikrostrukturellen Aspekte der Schaedigung (Working Life Predictions for Particle-Strengthened Aluminum Alloy with Regard to Microstructural Aspects of Deterioration)

    National Research Council Canada - National Science Library

    Berger, Wolfgang


    ... and., therefore, influence deterioration testing in preserved working life prediction concepts, thereby creating the basis for an improved application of these concepts for materials with heterogenous micro structures...

  3. Electric Energy Consumption of the Full Scale Research Biogas Plant “Unterer Lindenhof”: Results of Longterm and Full Detail Measurements

    Directory of Open Access Journals (Sweden)

    Thomas Jungbluth


    Full Text Available This work thoroughly evaluates the electric power consumption of a full scale, 3 × 923 m3 complete stirred tank reactor (CSTR research biogas plant with a production capacity of 186 kW of electric power. The plant was fed with a mixture of livestock manure and renewable energy crops and was operated under mesophilic conditions. This paper will provide an insight into precise electric energy consumption measurements of a full scale biogas plant over a period of two years. The results showed that a percentage of 8.5% (in 2010 and 8.7% (in 2011 of the produced electric energy was consumed by the combined heat and power unit (CHP, which was required to operate the biogas plant. The consumer unit agitators with 4.3% (in 2010 and 4.0% (in 2011 and CHP unit with 2.5% (in 2010 and 2011 accounted for the highest electrical power demand, in relation to the electric energy produced by the CHP unit. Calculations show that 51% (in 2010 and 46% (in 2011 of the total electric energy demand was due to the agitators. The results finally showed the need for permanent measurements to identify and quantify the electric energy saving potentials of full scale biogas plants.

  4. Environmental protection and penal law in Greece - a comparison with the German penal code on environmental matters. Der strafrechtliche Umweltschutz in Griechenland unter besonderer Beruecksichtigung des Deutschen Umweltstrafrechts

    Energy Technology Data Exchange (ETDEWEB)

    Karamanidis, G.


    The first chapter outlines the ecological situation of Greece, while the second chapter presents the legal foundations of environmental protection in Greece. Secondary laws are mentioned, as these are generally the laws in which penal liabilities are stated. The present environmental protection regulations are found to be unsatisfactory and unfit for preventing environmental damage. A new legislative structure is proposed on the basis of German environmental protection standards. (orig./HSCH).

  5. Experimental determination and numerical simulation of viscositites in slag-systems under gasification conditions; Experimentelle Bestimmung und numerische Simulation von Viskositaeten in Schlackesystemen unter Vergasungsbedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Nentwig, Thomas


    Silica melts are reflected in many different industrial and natural processes such as slags in steel production and gasification of coal, glass in the glass making industry, lava in the volcanology and in lots of other fields. Rheological properties like the viscosity of this silica melts are really important in all of these processes. A general and good working viscosity model would help all scientists and engineers, who have to simulate and optimise these processes. First, it is important to have the possibility to measure viscosities of slags. As part of this PhD thesis a new high-temperature high-pressure rotational viscosimeter is developed for measurements up to {>=}1600 C and 20 bar. Particularly in relation to the coal gasification process this two parameters are really important, because coal gasification occurs under high temperatures and high pressures. With this new viscosimeter it is possible to measure slag viscosities under realistic gasification conditions. To show that the new viscosimeter works quite good viscosity measurements of four real slags have been done. The influence of pressure and atmosphere on viscosity have been examined. The measurements are also compared with different viscosity models. The Arrhenius- and Weymann-Equation have been determined and the correlation between viscosity and basicity of the slag has been evaluated. In the second part of the Phd thesis a new viscosity model is developed. The model is based on the structure inside the slag and consists in existing thermodynamic models. After a detailed literature research the model is developed for the SiO{sub 2}-Al{sub 2}O{sub 3}-Na{sub 2}O-K{sub 2}O system. The quality of this new model is estimated by comparing calculated viscosity values with measurements found during the literature research. The new model is also compared with other existing models for viscosity calculation.

  6. Swimming pools and shower rooms - sealing directly under the tiles avoids hygienically serious water accumulations. Schwimmbecken und Duschraeume - Abdichtung direkt unter den Fliesen vermeidet hygienisch bedenkliche Wasseransammlungen

    Energy Technology Data Exchange (ETDEWEB)



    Swimming pool seals are to carry out according to the DIN 18195 part 7 in which sealing with strips and foils is required; at the bottom of the pool a sufficiently dimensioned protective coating under the tiles and in the wall area using the following construction from the outside to the inside: tiles, mortar, face brickwork of at least half brick thickness and behind it a 4 cm shell joint. Then as the next layer follows the seal with foils or sealing strips. The total construction facing the water is thus with a total layer thickness of about 10 cm permanently exposed to water with all the thus resulting consequences. (orig.).

  7. Reconstruction of the Leudelange refuse incineration plant with integration of existing plant parts; Erneuerung der MVA Leudelange unter Integration vorhandener Anlagenteile

    Energy Technology Data Exchange (ETDEWEB)

    Buechner, Harm-Peter; Jolas, Uwe [EEW Energy from Waste GmbH, Helmstedt (Germany)


    SIDOR, a special purpose association for waste management in Luxembourg, manages the waste disposal of around two-thirds of Luxembourg's entire population. E.ON Energy from Waste (EEW) participated in SIDOR's tender process for both constructing as well as operating a new incineration line with an overall capacity of 150,000 t/a, which was accepted by SIDOR in October 2006. The new incineration line with an overall gross heat output of 67 MWth replaced the existing lines after completion. (orig.)

  8. Studienbegleitender Deutschunterricht in Polen – Eine Bedarfsanalyse unter Studierenden der Technischen Universität KoszalinTechnischen Universität Koszalin

    Directory of Open Access Journals (Sweden)

    Joanna Kic-Drgas


    The quickly changing social reality (arising from globalisation, increasing mobility, and the specialisation of many scientific fields also affects modern language teaching for special purposes and results in the students’ changing expectations and needs. This paper aims at presenting the needs and expectations of the students studying a foreign language in an academic setting, using the example of a German course at the Koszalin Technical University. The basis for the findings and their analysis is a survey conducted among the students enrolled in economics, informatics, mechanics and European studies. The answers received from the students point strongly to the need for specialisation, a direction that should influence modern language teaching.

  9. Dominanzverhalten bei Przewalskipferden in seminatürlicher Haltung im Kölner Zoo unter besonderer Berücksichtigung der Körpersprache


    Herberz, S.


    Dominanzverhalten sichert die soziale Rangordnung innerhalb einer Pferdeherde und trägt so zur friedlichen Konfliktlösung bei. Die Körpersprache ist hierbei mit festgelegten Droh- und Unterwerfungsgesten ein wichtiges Kommunikationsmittel. Im Rahmen dieser Arbeit wurden Beobachtungen an der Przewalskipferd-Herde des Kölner Zoos durchgeführt. Dabei wurde die Körpersprache der Wildpferde analysiert und ihr Dominanzverhalten auf Abhängigkeit von Situation und Alterskonstellation untersucht.

  10. Emission factors of modern wood-pellet heating units under typical heating conditions - Final report; Emissionsfaktoren moderner Pelletkessel unter typischen Heizbedingungen - Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Good, J.; Nussbaumer, T.


    This final report for the Swiss Federal Office of Energy (SFOE) reports on the results of measurements made concerning the emission factors of two modern wood-pellet heating units under typical heating conditions. Using simulations, typical operation in single-family homes and apartment blocks were examined. Emissions during the different phases of operation were examined. Systems with and without buffer storage were also examined. The minimum running times to be striven for are quoted which would lead to a reduction of emissions to an acceptable level. The characteristic operating modes for the two heating units and the results obtained for various emissions are presented and discussed.

  11. Ein Beitrag zur aquatischen Käferfauna von Walcheren, Niederlande (Insecta, Coleoptera) unter besonderer Berücksichtigung von Halophilen und Halobionten Arten

    NARCIS (Netherlands)

    Burmeister, E.-G.


    EINLEITUNG In den Jahren 1975, 1977 und 1978 konnten in den Sommermonaten zahlreiche Gewässer der Halbinsel Walcheren auf ihre aquatische Käferfauna hin untersucht werden. Walcheren, ehemals eine Insel in der Scheidemündung, gehört zur Provinz Zeeland und ist heute durch die Polderlandschaft

  12. Radiological diagnostics of abdomen and thorax. Image interpretation considering anatomical landmarks and clinical symptoms; Radiologische Diagnostik Abdomen und Thorax. Bildinterpretation unter Beruecksichtigung anatomischer Landmarken und klinischer Symptome

    Energy Technology Data Exchange (ETDEWEB)

    Krombach, Gabriele A. [Universitaetsklinikum Giessen (Germany). Klinik fuer Diagnostische und Interventionelle Radiologie; Mahnken, Andreas H. (ed.) [Universitaetsklinikum Marburg (Germany). Diagnostische und Interventionelle Radiologie


    The book on radiological diagnostics of abdomen and thorax - image interpretation considering anatomical landmarks and clinical symptoms - includes three chapters: (1) imaging of different parts of the body: thorax and abdomen. (II) Thorax: head and neck; mediastinum; heard and pericardium; large vessels; lungs and pleura; mamma. (III) Abdomen: liver; gall bladder and biliary tract; pancreas; gastrointestinal tract; spleen and lymphatic system; adrenal glands; kidneys and urinary tract; female pelvis; male pelvis.

  13. Self-management by firm, non-elastic adjustable compression wrap device [Translation of Druckmessungen unter Klettverschluss-Kompression - Selbstbehandlung durch feste, unelastische Beinwickelung


    Giovanni Mosti; Hugo Partsch


    Severe forms of chronic venous insufficiency and lymphedema require strong compression-pressure, which exceeds the pressure exerted by medical compression stockings (>40 mmHg). The aim was to investigate if patients are able to apply a Velcro-band compression device (Circaid Juxta Lite™) themselves with sufficient pressure. Thirty-one patients (CEAP C6=23, C5=5, C3=2, mixed ulcer=1) applied Juxta Lite™ on their own legs after a short instruction and were asked to readjust the pressure by thei...

  14. Synthesis of sulfates and sulfate derivates of selected metals under harsh conditions; Synthese von Sulfaten und Sulfatderivaten ausgewaehlter Metalle unter drastischen Bedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Neuschulz, Kai


    In the course of this work sulfates und sulfate derivates of selected metals were synthesized under harsh conditions. The obtained compounds, in which the metal ions often reveal unusual oxidation states, have been characterized. Two mixed valent vanadium(IV/V) oxide sulfates (VO){sub 4}(SO{sub 4}){sub 5}, and (VO){sub 3}(SO{sub 4}){sub 4} and a vanadium(V) oxide sulfate (VO){sub 2}(SO{sub 4}){sub 3} as well as a complex anionic vanadium(V) oxide sulfate (NO)[VO(SO{sub 4}){sub 2}] have been obtained starting from vanadium or vanadium(V) oxide and oleum. All vanadium oxide sulfates reveal a similar thermal behavior. The decomposition residues are either pure vanadium(V) oxide or vanadium(IV) oxide or a mixture of both residues in different ratios. It was possible to synthesize binary tantalum(V) sulfate Ta{sub 2}(SO{sub 4}){sub 5} for the first time by reaction of pure sulfur(VI) oxide and tantalum(V) oxide at 150 C and to characterize. The complete absence of any oxide ligands at the metal center is of special interest in this compound. The reaction of tin(II) sulfate with oleum lead to the formation of tin(IV) sulfate Sn(SO{sub 4}){sub 2}. Using IR spectroscopy, the oxidation state +IV was confirmed for tin, due to the absence of OH-band in the IR-spectra, which would have meant the oxidation state +II. Using methanesulfonic acid as solvent and reactant on different synthetic routes the anhydrous methanesulfonates Mn(CH{sub 3}SO{sub 3}){sub 2}, and Fe(CH{sub 3}SO{sub 3}){sub 2}, Ga(CH{sub 3}SO{sub 3}){sub 3}, In(CH{sub 3}SO{sub 3}){sub 3}, and Tl(CH{sub 3}SO{sub 3}){sub 3}, as well as VO(CH{sub 3}SO{sub 3}){sub 2} and SnCl{sub 2}(CH{sub 3}SO{sub 3}){sub 2} were obtained. For the element thallium it was possible to obtain two mixed anionic compounds with the composition TlX{sub 2}(CH{sub 3}SO{sub 3}) (X = Cl or Br). All methanesulfonates show a similar thermal decomposition behavior forming the metal oxide as decomposition product. The thallium compounds represent an exception, due to sublimation of the residue in the course of the analysis. In analogy to the methanesulfonates the first structurally characterized triflates could be obtained in the synthesis of pure triflic acid from chromium, molybdenum, thallium, tin, and lead. For chromium(III) two complex ionic compounds (H{sub 3}O){sub 3}[Cr(CF{sub 3}SO{sub 3}){sub 6}] and [Cr(H{sub 2}O){sub 5}Cl](CF{sub 3}SO{sub 3}){sub 2} have been synthesized. In M[Mo{sub 2}(CF{sub 3}SO{sub 3}){sub 5}].2CF{sub 3}SO{sub 3}H (M = Rb, Cs) a molybdenum dumbbell was synthesized which molybdenum in the oxidation state +II. Quantum mechanical calculations on the bonding situation in the molybdenum dumbbell were carried out. In addition, a ternary molybdenyl(V) triflate of the formula K{sub 2}[MoO(CF{sub 3}SO{sub 3}){sub 5}] was obtained and the magnetism was analyzed. For the elements tin and lead compounds of the formula Sn(CF{sub 3}SO{sub 3}){sub 2}, and Pb(CF{sub 3}SO{sub 3}){sub 2} could be obtained as well as the adducts Pb(CF{sub 3}SO{sub 3}){sub 2}(CF{sub 3}SO{sub 3}H), and Pb(CF{sub 3}SO{sub 3}){sub 2}(CF{sub 3}SO{sub 3}H){sub 2}. All triflates form layered structures and have been studied by thermal analysis.

  15. Ein Lehrbuch über Psychiatrie und Psychotherapie – Geschlechtsunterschiede unter der Lupe? A Textbook for Psychiatry and Psychotherapy—Gender Differences under the Microscope?

    Directory of Open Access Journals (Sweden)

    Isabella Heuser


    Full Text Available Anke Rohde und Andreas Marneros haben sich ein hohes Ziel gesetzt: ein übersichtliches Lehrbuch für Psychiatrie und Psychotherapie, das erstmals eine systematische Zusammenstellung des Wissens über geschlechtsspezifische Aspekte von Symptomatologie, Epidemiologie, Diagnostik, Pharmakotherapie und Psychotherapie psychischer Störungen enthält. Bemerkenswert ist dabei zweierlei: Erstens werden bei der Betrachtung geschlechtsspezifischer Unterschiede im Sinne der Genderforschung Frauen und Männer berücksichtigt, zweitens wird ein vollständiger Überblick sowohl über die häufigen und bekannten psychischen Störungen als auch die äußerst seltenen psychiatrischen Erkrankungen der gesamten Altersspanne gegeben. Wenngleich die Qualität der einzelnen Beiträge deutlich zwischen sehr differenzierter und leider auch für ein Handbuch eindeutig zu oberflächlicher Betrachtung variiert, liegt in dem Band ein weitgehend informatives Nachschlagewerk vor, das einen ersten Überblick über die geschlechtsspezifische Psychiatrie gibt.Anke Rohde and Andres Marneros have given themselves a lofty goal: To put together a systematic collection for the first time that contains the entire body of knowledge on the gender-specific aspects of symptomatology, epidemiology, diagnostics, pharmacotherapy, and psychotherapy of psychological disturbances. There are two notable aspects: First, in accordance with contemporary gender analysis, the examination of the gender-specific differences takes both women and men into account; second, the book provides a complete overview of the common and well-known psychological disturbances as well as the extremely rare psychiatric illnesses along the entire age spectrum. Although the quality of the individual articles clearly ranges from the very sophisticated to a superficial treatment inappropriate for a textbook, the volume itself is, for the most part, an informative work of reference that offers an initial overview of gender-specific psychiatry.

  16. Time and environmental science. Pt. 2. Time and fundamental concepts; Die Bedeutung der Zeit. T. 2. Die Umweltwissenschaften im Kontext von Zeit: Begriffe unter dem Aspekt der Zeit

    Energy Technology Data Exchange (ETDEWEB)

    Kuemmerer, K. [Inst. fuer Umweltmedizin und Krankenhaushygiene, Freiburg (Germany); Held, M. [Evangelische Akademie, Tutzing (Germany)


    The consideration of time in environmental research allows new insights into the fundamentals of the environmental sciences. Considering temporalities, rhythms and time scales as well as their interdependencies with environmental science enables us to acquire a better understanding of such basic principles as irreversibility, persistence, disturbance, damage or resilience. Time scales and rhythms are of special importance for these principles and considerations. With regard to their persistence, especially the critical substances demonstrate reactivities which are so low that they are stable enough for application, although these reactivities are still seen to be present to a certain degree and they reveal a rate of transport which is not too high. In this manner, persistence is a factor which is dependent on the particular circumstances. Whether changes are to be classified as reversible or irreversible is dependent on the particular time period and the specific systems, their description and the parameters hereby selected. A prerequisite for the continuing existence of a system is, among other things, that this system must demonstrate a specific rate of development and must take into account the interconnectedness of the various time scales. Ecological systems can continue to develop according to their own dynamics if disturbances can be avoided which lead, compared to their own time scales, to rapid alterations and consequently to critical fluctuations. The duration of such disturbances should be as short as possible in order to minimize the rapid anthropogenic changes in an ecosystem as compared with the systems own time scales. (orig.) [Deutsch] Zeit in umweltwissenschaftliche Fragestellungen einzubeziehen, ermoeglicht ein neues Verstaendnis grundlegender Begriffe der Umweltwissenschaften sowie neue Zugaenge zu oekologischen Fragestellungen. Dies betrifft Vorstellungen und Kriterien, die fuer die Forschung, aber auch die praktische Umsetzung der Forschungsergebnisse von grundlegender Bedeutung sind wie z.B. Persistenz von Schadstoffen, Irreversibilitaet von Veraenderungen, Schaden und Schaedigung und auch Elastizitaet oder Resilience von Oekosystemen. Insbesondere Zeitskalen und Rhythmen sowie deren Vielfalt sind dafuer von Bedeutung. Hinsichtlich ihrer Persistenz besonders kritische Stoffe zeichnen sich dadurch aus, dass ihre Reaktivitaet zwar so gering ist, dass sie fuer die Anwendung stabil genug sind, die Reaktivitaet aber noch in gewissem Mass vorhanden ist und sie eine nicht zu hohe Transportrate besitzen. Persistenz ist somit ein vom Kontext abhaengiges Kriterium. Ob Aenderungen als reversibel oder irreversibel einzustufen sind, ist von den betrachteten Zeitraeumen und jeweiligen Systemen, deren Beschreibung und den hierfuer ausgewaehlten Parametern abhaengig. Voraussetzungen fuer den Fortbestand eines Systems zu schaffen, bedeutet u.a. die systemeigene Entwicklungsgeschwindigkeit und die Verschraenktheit unterschiedlicher Zeitskalen zu beachten. Wenn Stoerungen, die zu schnellen Aenderungen und somit zu kritischen Fluktuationen fuehren, vermieden werden, koennen sich oekologische Systeme entsprechend ihrer eigenen Dynamik weiterentwickeln. Die Dauer von Stoerungen sollte moeglichst kurz sein, um anthropogene, im Vergleich zur systemeigenen Zeitskala schnelle Veraenderungen von Oekosystemen zu minimieren. (orig.)

  17. Corrosion by sulfate-reducing bacteria in a HP gas line under a detached weld cladding; Korrosion durch sulfatreduzierende Bakterien an einer Hochdruckgasleitung unter abgeloester Schweissnahtnachumhuellung

    Energy Technology Data Exchange (ETDEWEB)

    Bette, Ulrich [Technische Akademie Wuppertal (Germany)


    Intelligent pig measurements detected several points of corrosion in a HP gas pipeline in northern Germany. Corrosion occurred in a pipe section buried in clay soil, under detached weld claddings. It was not detected in regular measurements and additional intensive measurements. When the pipes were dug up, sulfate-reducing bacteria were found as the cause of corrosion. Due to the location of the corrosion processes, cathodic protection was impossible, and IFO measurements were ineffective in the low-ohmic soil.

  18. Strategies for the optimization of cathodic protection of pipelines under AC influence; Strategien fuer die Optimierung des kathodischen Korrosionsschutzes von Rohrleitungen unter Wechselspannungsbeeinflussung

    Energy Technology Data Exchange (ETDEWEB)

    Buechler, Markus [SGK Schweizerische Gesellschaft fuer Korrosionsschutz, Zuerich (Switzerland)


    The newly determined values for the protection of cathodically protected pipelines against AC corrosion cannot be readily applied to all pipelines. From this perspective, the author of the contribution under consideration reports on strategies for the optimization of cathodic protection of pipelines under AC influence. It is hardly possible to define general strategies. Rather, an examination of the entire pipeline is essential. Is a compliance with the prescribed limits not possible or only possible with unreasonable effort, an active cathodic corrosion protection in combination with remote-controlled monitoring and protection devices can reduce the current corrosion risk.

  19. Efficiency of heat pump systems in real operating conditions. Influence of storage concepts; Effizienz von Waermepumpensystemen unter realen Nutzungsbedingungen. Einfluss der Speicherkonzepte

    Energy Technology Data Exchange (ETDEWEB)

    Miara, Marek; Guenther, Danny [Fraunhofer-Institut fuer Solare Energiesysteme (ISE), Freiburg im Breisgau (Germany). Abt. Thermische Anlagen und Gebaeudetechnik


    Heat pump systems are used increasingly in space heating and freshwater heating. This is reflected in their fast-growing market shares in new buildings. There is a similar trend in modernisation of buildings and in the replacement of fossil-fuel heating systems. Investors appear to be convinced by the advantages of heat pumps.

  20. Die Evagriusübersetzung der Vita Antonii : Rezeption - Überlieferung - Edition : Unter besonderer Berücksichtigung der Vitas Patrum-Tradition

    NARCIS (Netherlands)

    Bertrand, P.H.E.


    The Life of Antony, translated by Evagrius of Antioch: Reception, Manuscript Tradition, Edition. With special emphasis on Vitas Patrum Tradition The Life of Antony is the first and one of the most famous saints’ lives that has ever been written. Shortly after the death of the desertfather

  1. Lifetime assessment of thick-walled components made of nickel-base alloys under near-service loading conditions; Lebensdauerbewertung dickwandiger Bauteile aus Nickelbasislegierungen unter betriebsnahen Beanspruchungen

    Energy Technology Data Exchange (ETDEWEB)

    Hueggenberg, Daniel


    Until 2050 the renewable energies should provide 80% of the power in Germany according to Renewable Energy law. Due to that reason the conventional power plants are not used for base load, but rather for the supply of average and peak load. The change of the operating mode leads to shorter times at stationary temperatures and the number of faster start-ups/shut-downs of the power plants will increase. As a result of this the components are exposed to an interacting load of creep and fatigue which reduces the lifetimes. The aim of this thesis is the development and verification of a lifetime assessment procedure for components made of the nickel-base alloys Alloy 617 mod. and Alloy 263 under creep fatigue loading conditions based on numerical phenomenological models and on the approaches of different standards/recommendations. The focus lies on two components of the high temperature material test rig II (HWT II), a header made of Alloy 617 mod. and Alloy 263 as well as a formed part made of Alloy 617 mod. For the basis characterization of the HWT II melts, specimens of the Alloy 617 mod. and Alloy 263 are tested in uniaxial tensile tests, (creep-)fatigue tests, creep tests and charpy tests in a temperature range between 20 C and 725 C. From the comparisons of the test results and the material specifications respectively the results of the projects COORETEC DE4, MARCKO DE2 and MARCKO700 no deviations were obvious for both materials with the exception of the creep test results with Alloy 617 mod. material. The creep tests with Alloy 617 mod. material of the HWT II melt show differences regarding the deformation and damage behavior. In addition to the basis characterization tests some complex lab tests for the characterization of the material behavior under creep-fatigue and multiaxial loading conditions were conducted. The developments of the microstructure, the precipitations as well as the structure of dislocations are investigated in the light optical microscope and the transmission electron microscope for the base raw material, the creep and creep-fatigue exposed material. For the classification the investigation results were compared to the results of the other projects and no differences could be identified. For the description of the deformation and damage behavior under creep-fatigue loading with finite elements simulations a viscoplastic deformation model with an integrated damage model of Lemaitre was used. The material dependent model parameters were fitted under consideration of the basis characterization test results of the Alloy 617 mod. and Alloy 263. All basis characterization tests are simulated with finite elements to classify the parameter fittings. The verification of the fitted material models was carried out by simulations of the complex lab tests. From the comparison of the simulation and test results it is obvious that the deformation and damage behavior can be reproduced with the used material model in a good manner. With finite element simulations of complex thick-walled components (header, formed part) under realistic thermal and mechanic loading conditions could be shown that the viscoplastic material model fitted for the Alloy 617 mod. and Alloy 263 is able to predict the locations of the maximum loadings and the lifetime until the first cracks appear. This could be confirmed by dye penetrant testing on the one hand and destructive investigations of two fracture surfaces of the header on the other hand. Additionally the approaches of the European DIN EN 12952-3/4, the American ASME Section III Division 1 Subsection NH, the French RCC-MR RB 3262.12 and the British R5 recommendations Volume 2/3 are used to predict the lifetimes. It can be seen that the approaches of ASME and RCC-MR provide very conservative predictions and that the approaches of R5 and DIN EN 12952 provide non-conservative predictions. These results lead to the conclusion that no approach of the standards/recommendation is suitable for the nickel base alloys Alloy 617 mod. and Alloy 263.

  2. Prevention, screening and therapy of thyroid diseases and their cost-effectiveness; Praevention, Screening und Therapie gutartiger Schilddruesenerkrankungen unter dem Aspekt von Kosten und Nutzen

    Energy Technology Data Exchange (ETDEWEB)

    Dietlein, M.; Moka, D.; Schmidt, M.; Theissen, P.; Schicha, H. [Koeln Univ. (Germany). Klinik und Poliklinik fuer Nuklearmedizin


    Cost-effectivness analyses focused on benign thyroid diseases are under-represented in the literature. The calculation of costs per additionally gained life year is difficult: The benefit of prevention is shifted into the distant future. The influence of an untreated subclinical thyroid disease on life expectancy can only be demonstrated by a long-term follow-up and by epidemiological databases. Iodine supplementation and programs for the prevention of tobacco smoking (primary prevention) are very cost-effective. Smoking increases the risk both of multinodular goiter and of Graves' disease. Screening programs (secondary prevention) are discussed for the laboratory parameters thyrotropin (TSH), calcium and calcitonin. TSH testing seems to be very cost-effective for epidemiological considerations in a certain lifespan (newborn, pregnancy, postpartal), older persons, hospitalisation due to acute diseases and in persons with previously elevated TPO-antibodies for TSH-values >2 mU/l, but dedicated cost-effectiveness analyses are lacking. On the other hand, the cost-effectiveness of a routine TSH testing beyond the age of 35 years has been shown by a high-quality decision analysis. Therapeutic strategies (tertiary prevention) aim at the avoidance of complications (atrial fibrillation, myocardial infarction, death for cardiac reasons) and of iatrogenic complications. Examples of a tertiary prevention are: firstly the definitive therapy of Graves' disease in patients who have on increased risk of relapse after antithyroid drugs (ATD), secondly the radioiodine therapy for subclinical hyperthyroidism and the radioiodine therapy of large goiters in older patients or in patients suffering from a relevant comorbidity. Cost-effectiveness analyses for different therapeutic strategies of Graves' disease were published using a lifelong time-horizon. The ablative radioiodine dose-regime is cost-effective as a fist line therapy if the risk of relapse after ATD exceeds 60%. (orig.) [German] Kosten/Nutzenanalysen in Bezug auf gutartige Schilddruesenerkrankungen sind in der Literatur unterrepraesentiert. Insbesondere die Erhebung eines Geldwertes pro gewonnenem Lebensjahr gestaltet sich methodisch schwierig: Der Nutzen praeventiver Massnahmen ist weit in die Zukunft verlagert. Der Einfluss einer unbehandelten Schilddruesenerkrankung auf die Lebenszeit wird ebenfalls erst durch einen langfristigen Horizont und dann eher epidemiologisch als individuell zu erfassen sein. Als Prophylaxe (primaere Praevention) sind Programme zum Ausgleich des Iodmangels sowie die Aufklaerung ueber den negativen Einfluss des Rauchens auf die Entwicklung einer Struma oder eines M. Basedows aus entscheidungstheoretischer Sicht sehr kosteneffektiv. Screening-Programme (sekundaere Praevention) werden fuer die Parameter TSH, Calcium und Calcitonin diskutiert. Eine besonders guenstige Kosten-Effektivitaet des TSH-Screenings ist in besonderen Lebensphasen (Neugeborene, Schwangerschaft, postpartal, hoeheres Alter, stationaerer Patient mit akuter Erkrankung) und bei definierten Vorbefunden (TSH>2mU/l, TPO-Antikoerper) aus klinisch-epidemiologischer Sicht zu erwarten, ohne dass hierzu gesonderte gesundheitsoekonomische Berechnungen vorgelegt wurden. Andererseits konnte die Kosten-Effektivitaet eines generellen TSH-Screening ab dem 35. Lebensjahr in einer qualitativ hochwertigen gesundheitsoekonomischen Studie bereits belegt werden. Die Therapiestrategien bei gutartigen Schilddruesenerkrankungen (tertiaere Praevention) zielen auf eine Minimierung sekundaerer Krankheitsfolgen (Vorhofflimmern, Myokardinfarkt, Herztod) und iatrogener Nebenwirkungen. Beispiele fuer eine solche tertiaere Praevention sind die definitive Therapie (Radioiodtherapie) der Immunhyperthyreose M. Basedow bei unguenstigen initialen Praedikatoren fuer eine Rezidivhyperthyreose, die Radioiodtherapie der latenten Hyperthyreose sowie die Radioiodtherapie der grossvolumigen Struma bei aelteren oder multimorbiden Patienten. Hierzu liegen Kosten-Effektivitaets-Analysen mit einem lebenslangen Zeithorizont zur Behandlung der Immunhyperthyreose M. Basedow vor. Die Daten sprechen fuer den primaeren Einsatz der Radioiodtherapie bei solchen Patienten, deren Rezidivrisiko nach thyreostatischer Behandlung ueber 60% liegt. (orig.)

  3. Land has power. Energy transition an opportunity for rural areas. Documentation; Land unter Strom. Die Energiewende als Chance fuer den laendlichen Raum. Dokumentation

    Energy Technology Data Exchange (ETDEWEB)

    Bredenbeck, Martin; Gotzmann, Inge (comps.)


    The energy transition affects the rural areas of Germany in particular. Which Opportunities and risks this brings there shows a new release of Bund Heimat und Umwelt in Deutschland (German heritage and Environmental Foundation (BHU)). The Federation of heritage and civic associations devoted to the question of how to the conventional expansion of renewable energies innovative options can added. Thereby a diversified positioning of energy use, the advancement of historical forms of use and the spatial differentiation are important factors. [German] Die Energiewende betrifft die laendlichen Raeume Deutschlands in besonderem Masse. Welche Chancen und Risiken sie dort bringt, zeigt eine Neuerscheinung des Bund Heimat und Umwelt in Deutschland (BHU). Der Bundesverband der Heimatverbaende, Heimat- und Buergervereine widmet sich der Frage, wie zum konventionellen Ausbau erneuerbarer Energien innovative Optionen hinzutreten koennen. Eine breite Aufstellung von Energienutzungen, die Weiterentwicklung historischer Nutzungsformen und die raeumliche Differenzierung sind dabei wichtige Faktoren.

  4. Analysis of injection sprays by means of large high-speed engines under cold and evaporating conditions; Analyse von Einspritzsprays mittelschnelllaufender Grossmotoren unter kalten und verdampfenden Bedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Fink, Christian; Pinkert, Fabian; Harndorf, Horst [Rostock Univ. (Germany). Lehrstuhl fuer Kolbenmaschinen und Verbrennungsmotoren; Frobenius, Moritz [AVL Deutschland GmbH, Haimhausen (Germany)


    The introduction of future emission limits for marine diesel engines requires an improved understanding of the fuel injection process and mixture formation as well as the development of robust and powerful tools to calculate the emission generation. This publication presents research results of the project partners AVI Deutschland GmbH and Rostock University obtained in the associated project EMI-MINI II. Objective of the experimental part of the work is the fundamental investigation of the mixture formation at cold and evaporative conditions in a high-pressure and high-temperature chamber by means of optical methods. For the measurements, a modem, heavy fuel capable single circuit common-rail injector of a medium speed diesel engine is applied. Based on the experimental results a calibration and validation of spray break-up and evaporation models is done. The models are applied in a specific simulation approach in order to predict engine emissions. Characteristic feature of the chosen approach is the consideration of specific nozzle internal flow conditions as boundary conditions for the spray break-up models and thus for the ignition, combustion and emission generation. The presented results demonstrate that the applied models are very sufficient to calculate injection and mixture formation processes. By comparing calculated engine emissions to experimental results obtained by WTZ Rosslau gGmbH it is shown that the presented simulation approach is capable to predict the impact of varying engine parameters on NO{sub x}- and soot emissions effectively. (orig.)

  5. Entwicklung einer Methode zur monetären Bewertung des Wassers für ein Unternehmen unter Einbeziehung des Wasser-Fußabdrucks


    Hentschel, Nora


    Wassernutzung und Abbau der Frischwasser-Ressourcen sind heutzutage ein Thema, das als so wichtig wahrgenommen wird wie der Klimawandel. Wasser wird somit zunehmend Auswirkungen auf den ökonomischen Wert von Unternehmen haben. Ziel der vorliegenden Arbeit ist es, den monetären Wert des Wassers mithilfe des Wasser-Fußabdrucks zu bestimmen. Hierfür werden nach einer kurzen Einführung in Kapitel 2 der ökologische Fußabdruck, der CO2-Fußabdruck und der Wasser-Fußabdruck kurz vorgestellt und in...

  6. Unter welchen Umständen würden deutsche Landwirte gentechnisch veränderten Raps anbauen? Ein Discrete-Choice-Experiment


    Breustedt, Gunnar; Muller-Scheessel, Jorg; Meyer-Schatz, Henrika Marie


    We examine the factors affecting the willingness of German farmers to adopt genetically modified (GM) oilseed rape after the pending commercial release of GM varieties. The analysis is based mainly on a web-based Discrete Choice Experiment with 217 oilseed rape growers in Germany. The determinants of adoption were estimated with the use of a multinomial probit model. Results indicate a significant impact of economic determinants on the adoption willingness: the difference in gross margins bet...

  7. Ecology trends in Greece and in the Federal Republic of Germany. Oekologiebewegung in Griechenland und in der Bundesrepublik Deutschland; Unter Beruecksichtigung historischer, gesellschaftspolitischer und theoretischer Aspekte

    Energy Technology Data Exchange (ETDEWEB)

    Dede, I.


    This study evolves and discusses a thesis which maintains that ecology trends, both in Greece and in the Federal Republic of Germany, are the results of specific processes of social ''modernization'' based on different historical developments. A definition of ''modernization'' is given discussing the ''Fordism'' conception as part of the regulation theory: ''new'' social trends are interpreted as the results of the practice and crisis of Fordistic capitalism which, however, differs depending on its ''metropolitan'' (Germany) and ''peripheral'' (Greece) manifestations. The applicability of the Fordism theorem to Greece is discussed taking into account some political and cultural parameters. (orig.)

  8. Automation and communication in the underground logistic area. Process and technology; Automatisierung und Kommunikation im Bereich Logistik unter Tage. Prozesse und Technik

    Energy Technology Data Exchange (ETDEWEB)

    Giesselmann, Thomas [Zentralstab Kernbereich, RAG Deutsche Steinkohle AG, Herne (Germany); Ball, Roland [Bergwerk Auguste Victoria, RAG Deutsche Steinkohle AG, Marl (Germany)


    A trendsetting change in German deep coal mining took place after the political decisions relating to coal in 2007. Besides the efforts at productivity increase and cost reduction, future-proof reshaping of mining under changing basic conditions is in order. A rich field of infrastructure activity, particularly in transport logistics, is to be found here, the more so as around 50% of the underground strata are needed. Though the technical prerequisites for transport logistics improvement, like automated diesel cars, PDA use, and WLAN networks, have been created in recent years, today's focus is on transparent process structuring for the entire logistics chain and the expedient combination and use of the multitude of existing and newly acquired process data. An initial project at the Auguste Victoria colliery, namely the equipment of underground transportation means with machine and speech communicators and the installation of a logistics control room, should yield confirmation of the expected improvements and functionalities. (orig.)

  9. Magneto-optical measurements on high-temperature superconductors influenced by AC-fields; Magnetooptische Untersuchungen an Hochtemperatursupraleitern unter Einfluss von Frequenzfeldern

    Energy Technology Data Exchange (ETDEWEB)

    Che' Rose, Simon


    In this work magneto-optical measurements on YBa{sub 2}Cu{sub 3}O{sub 7-x} and MgB{sub 2} thin films were done. For YBCO the influence of AC-pulses on the flux and current density of a thin film with transport current was investigated. For MgB{sub 2} the influence of AC-fields on the homogenous and dendritic flux penetration was researched. (orig.)

  10. Pyrolysis of petroleum residues while making use of the hydrogen potential of polymer waste; Pyrolyse von Erdoelrueckstaenden unter Nutzung des Wasserstoffpotentials von Polymerabfaellen

    Energy Technology Data Exchange (ETDEWEB)

    Klemens, I; Butz, T; Rahimian, I; Linde, A [Institut fuer Erdoel- und Erdgasforschung, Clausthal-Zellerfeld (Germany)


    The hydrogen potential contained in waste plastics is to be utilized optimally for cracking of petroleum residues. Homogeneous distribution of the plastic particles in the petroleum residue is to ensure short distances between the hydrogen donor and acceptor, a sufficently large reaction surface, and stable mixing for further processing. Apart from homogeneity, another factor of interest is the influence of mixed-in polymer particles on the colloidal stability of the petroleum residue and the physico-chemical properties of the mixtures. With a view to further processing, the mixtures were analyzed for high-temperature stability, flow characteristics, and reaction behaviour during pyrolysis. (orig.) [Deutsch] Ziel dieses Vorhabens ist es, das in den Kunststoffabfaellen vorhandene Wasserstoffpotential optimal fuer eine tiefergehende Spaltung der Erdoelrueckstaende nutzbar zu machen. Durch eine homogene Verteilung der Kunststoffpartikel im Erdoelrueckstand soll ein moeglichst kurzer Transportweg zwischen Wasserstoff-Donor und -Akkzeptor, eine ausreichend grosse Reaktionsoberflaeche und eine fuer weitere Verarbeitungsschritte notwendige stabile Mischung erreicht werden. Ebenso interessant wie die Homogenitaet solcher Gemische ist auch der Einfluss der eingemengten Polymerteilchen auf die Kolloidstabilitaet des Erdoelrueckstandes und die physikalisch-chemischen Eigenschaften der Mischungen. Im Hinblick auf eine technische Weiterverarbeitung werden die Gemische besonders auf ihre Stabilitaet bei erhoehten Temperaturen (Heisslagerstabilitaet), ihr Fliessverhalten sowie ihr Reaktionsverhalten waehrend der Pyrolyse untersucht. (orig.)

  11. Approaches to the dimensioning of enhanced biological phosphorus elimination systems, taking dynamic simulation into account; Bemessungshinweise zur vermehrten biologischen Phosphorelimination unter Beruecksichtigung der dynamischen Simulation

    Energy Technology Data Exchange (ETDEWEB)

    Scheer, H.


    With so many projects either planned or under construction, the question of the dimensioning of sewage treatment plants with enhanced biological phosphorus elimination (BIO-P) is becoming more and more important. A detailed search of literature established in how far dimensioning approaches or models were already available in the spring of 1994. These modelling approaches were critically examined and compared as to their practical applicability by means of parameter and sensitivity studies. For this purpose, they were programmed and the relevance of certain dimensioning parameters to biological phosphorus elimination was studied by means of a pilot plant. (orig./SR) [Deutsch] Der Auslegung von Klaeranlagen mit vermehrter biologischer Phosphorelimination (BIO-P) kommt bei der Vielzahl von Planungs- und Baumassnahmen eine immer wichtigere Bedeutung zu. Inwieweit fuer die Bemessung von Klaeranlagen mit BIO-P im Fruehjahr 1994 bereits auf vorhandene Bemessungsansaetze und -modelle zurueckgegriffen werden konnte, wurde mittels einer detaillierten Literaturstudie, untersucht. Diese Modellansaetze wurden im Hinblick auf ihre praxisorietierte Anwendbarkeit durch Parameter- und Sensitivitaetsstudien kritisch untersucht und verglichen. Hierzu wurden die verschiedenen, zum damaligen Zeitpunkt vorhandenen Ansaetze programmiert und die Auswirkungen wichtiger bemessungsrelevanter Parameter auf die BIO-P anhand einer Modellklaeranlage abgeschaetzt. (orig./SR)

  12. Reducing particulate matter in the operation of firewood burning stoves taking into account the toxicological relevance; Feinstaubminderung im Betrieb von Scheitholzkaminoefen unter Beruecksichtigung der toxikologischen Relevanz

    Energy Technology Data Exchange (ETDEWEB)

    Lenz, Volker Alfred


    One of the greatest challenges facing humanity is climate change. Correspondingly, inter alia, the German government has set a target by 2020, to reduce emissions of greenhouse gases to the 1990 level by 20%. For this purpose can and should an increased energetic use of biomass contribute. End of 2007, the bioenergy had a share of around three quarters of the renewable primary energy input. Of which more than 45% were used for the heat supply. A total of more than 90% of renewable heat have been provided from biomass. From the provided amount of heat come over 80% from the combustion of solid biofuels - so far almost exclusively wood products - in small and medium wood-fuelled combustion systems. To reduce carbon dioxide emissions the federal government is accelerating a further expansion of energetic use of biomass in the heating sector. This expansion of thermal use of biomass, however, for reasons of pollution control, should not rise simultaneously with the emissions of air pollutants such as carbon monoxide, nitrogen oxides or particulate matter. [German] Eine der groessten Herausforderungen fuer die Menschheit ist der Klimawandel. Entsprechend hat sich u.a. die deutsche Bundesregierung das Ziel gesetzt bis 2020 die Emissionen an Treibhausgasen im Vergleich zum Stand 1990 um 20 % zu mindern. Hierzu kann und soll eine verstaerkte energetische Nutzung der Biomasse beitragen. Ende 2007 hatte die Bioenergie einen Anteil von rund drei Viertel am erneuerbaren Primaerenergieeinsatz. Davon wurden mehr als 45 % fuer die Waermebereitstellung genutzt. Insgesamt wurden mehr als 90 % der erneuerbaren Waerme aus Biomasse bereitgestellt. Von der bereitgestellten Waermemenge kommen ueber 80 % aus der Verbrennung von festen Biobrennstoffen - bisher fast ausschliesslich Holzprodukte - in kleinen und mittleren Holzfeuerungsanlagen. Zur Verminderung der Kohlenstoffdioxidemissionen wird von Seiten des Bundes ein weiterer Ausbau der energetischen Biomassenutzung im Waermebereich forciert. Bei diesem Ausbau der thermischen Biomassenutzung duerfen aber aus Gruenden des Immissionsschutzes nicht gleichzeitig die Emissionen an Luftschadstoffen wie z. B. Kohlenstoffmonoxid, Stickstoffoxide oder Feinstaub ansteigen.

  13. Simulation of a model predictive room temperature control by use of an ideal weather forecast; Simulation einer praediktiven Raumtemperaturregelung unter Verwendung einer idealen Wettervorhersage

    Energy Technology Data Exchange (ETDEWEB)

    Goertler, Gregor [Fachhochschulstudiengaenge Burgenland GesmbH, Pinkafeld (Austria). Kernkompetenzbereich Energie- und Umweltmanagement; Beigelboeck, Barbara


    Due to the use of MPC (Model Predictive Control) for room heating applications users and constructors expect nameable energy savings. By usage of a simulation for a special case the energy saving potential of predictive control algorithm for room temperature control in connection with an ideal weather forecast, in comparison to established algorithms (PI-control, two level controller) is estimated. The controlled system with the control variable room temperature is a room with floor heating which was modelled in TRNSYS. A linear state space model of the controlled system was derived with suitable identification methods. This model was used by the predictive control algorithm, which was programmed in MATLAB. The weather data was taken from the TRNSYS library and has been made available also for the control algorithm, so that an ideal weather forecast was established. For the example considered in this paper, the amount of energy saving was 10 % per year with the MPC-controller compared to a PI-controller. (Copyright copyright 2010 Ernst and Sohn Verlag fuer Architektur und technische Wissenschaften GmbH and Co. KG, Berlin)

  14. Paracetamol overdose: the liver unit perspective.

    LENUS (Irish Health Repository)

    Iqbal, M


    Liver failure resulting from deliberate or accidental paracetamol overdose continues to be an important reason for referral to liver transplant centres. Severe hepatic dysfunction often appears 72-96 h after overdose. Liver injury can be prevented by timely administration of the specific antidote, N-acetylcysteine. Unfortunately, administration of N-acetylcysteine is frequently delayed due to late presentation or late administration. While N-acetylcysteine works best if given within 8 h of overdose, it is beneficial at any time period and should always be given if there is concern about significant overdose, irrespective of interval from time of ingestion. Early discussion with liver transplant unit is suggested if there is any doubt or evidence of liver failure.

  15. The Use of Drugs to Reduce Hearing Loss Following Acute Acoustic Trama (United States)


    noise exposure: (1) L-N- acetylcysteine (L-NAC); (2) D-Methionine (D-MET); (3) Ebselen SPI-1005; (4) Acetyl-L-carnitine (ALCAR) and (5) Src-PTK to a 4.0 kHz octave band of noise for 6 hours at 105 dB SPL: (1) L-N- acetylcysteine (L-NAC); (2) D-Methionine (D-MET); (3) Ebselen SPI-1005...effectively absent or severely depressed from 2 kHz and above (Figs. 14 & 15). Individual cochleograms for this group are shown in Figure 16. The

  16. The Use of Drugs to Reduce Hearing Loss Following Acute Acoustic Trauma (United States)


    compare, in the rescue mode of treatment, the effectiveness of (1) L-N-acetylcysteine (L- NAC); (2) D-Methionine (D-MET); (3) Ebselen SPI-1005; (4) Acetyl...trauma: (1) L-N-acetylcysteine (L-NAC); (2) D-Methionine (D-MET); (3) Ebselen SPI-1005; (4) Acetyl-L-carnitine (ALCAR) and (5) Src-PTK inhibitor, KX1...octave band of noise at 108 dB SPL and treated in the rescue mode with (i) L-NAC, (ii) D-MET, (iii) Ebselen SPI-1005, (iv) ALCAR, or (v) Src-PTK

  17. Lifelong physical activity prevents an age-related reduction in arterial and skeletal muscle nitric oxide bioavailability in humans

    DEFF Research Database (Denmark)

    Nyberg, Michael Permin; Blackwell, James R; Damsgaard, Ramsus


    studied the effect of ROS on systemic and skeletal muscle NO bioavailability and leg blood flow by infusion of the antioxidant N-acetylcysteine (NAC). Infusion of NAC increased the bioavailability of NO in OS, as evidenced by an increased concentration of stable metabolites of NO (NOx) in the arterial...

  18. Exogenous lipoid pneumonia caused by Nigella sativa oil – A case report

    Directory of Open Access Journals (Sweden)

    K. Bouti


    The patient was advised to stop taking N. sativa oil. The only treatment that was instituted was N-acetylcysteine. A clinically significant change in symptoms and chest radiograph was observed. The patient has remained stable 18 months after the diagnosis.

  19. Intravenøs eller oral N-acetylcysteinbehandling til paracetamolforgiftede patienter. Er det tid til at revurdere behandlingsinstruksen?

    DEFF Research Database (Denmark)

    Rolff, Hans Christian; Christensen, Hanne Rolighed; Dalhoff, Kim


    Danish paracetamol (PCM) poisoned patients are treated with N-acetylcysteine (NAC) intravenously for 36 hours. This probably leads to overtreatment. Today, patients with poor prognoses can be identified and, in addition, NAC may have serious side effects. We reviewed the literature (route...

  20. Survival in patients with amyotrophic lateral sclerosis, treated with an array of antioxidants

    NARCIS (Netherlands)

    Vyth, A.; Timmer, J. G.; Bossuyt, P. M.; Louwerse, E. S.; de Jong, J. M.


    Between 1983 and 1988 we treated 36 patients with sporadic amyotrophic lateral sclerosis (ALS) by an array of antioxidants and added other drugs to the regimen whenever a patient reported deterioration. Our customary prescription sequence was N-acetylcysteine (NAC); vitamins C and E;

  1. Oxidative stress and lung function profiles of male smokers free from ...

    African Journals Online (AJOL)


    Jun 12, 2014 ... continues to cause heavy health and economic burden around ... smoking is associated with increased oxidative stress in the lungs (7, 8). ... The present study aims to investigate the blood and ... from COPD compared to smokers with COPD (among ... oral or inhaled N-acetylcysteine or vitamin C and/or E.

  2. Chemical Protection Against Ionizing Radiation. (United States)


    cystathionine [3763. Exogen- ous sources of cysteine such as - acetylcysteine and L-2-oxothiazolldine-4 carboxylate promote glutathione synthesis. Whereas the...tubular epithelium and catalyzes the oxidation of extracellular thfols only. Substrates include GSH, cysteine, N- acetylcysteine and dithiothreitol [328,329...extracellular C&2+ . These studies have demonstrated the depression of Ca 2+ sequestration in liver microsomes and mitochondrta after treatment with

  3. Selection Criteria and Prioritization of Resuscitation Fluid Adjuvants (United States)


    blood pressure falls. Humans are likely to experience hypovolemic shock if hemorrhage depresses systolic blood pressure to less than 85 mmHg. Once blood...perfluorocarbon microbubbles, pyruvate, ethyl pyruvate, free radical scavengers, melatonin, N- acetylcysteine , TEMPOL (4-hydroxy-2,2,6,6

  4. Department of Defense Chemical and Biological Defense Program. Volume I: Annual Report to Congress (United States)


    nerve agents. * Identified specific gene products that are either enhanced or depressed in the brain following low-level chemical warfare nerve agent...with ample time to properly treat for injury. This model was used to determine that N- acetylcysteine given intraperitoneally increased survival rates in

  5. A Randomized Placebo-Controlled Trial of D-Cycloserine for the Enhancement of Social Skills Training in Pervasive Development Disorders (United States)


    hyperactivity disorder, oppositional defiant disorder, schizophrenia, ASD, social anxiety disorder, and major depression ). The child’s appropriateness for...4):958-964. 31. Hardan AY, Fung LK, Libove RA, et al. A randomized controlled pilot trial of oral N- acetylcysteine in children with autism. Biological

  6. Wpływ pojedynczej dawki paracetamolu i/lub N-acetylocysteiny na szczury przewlekle eksponowane na trichloroetylen. III. Wpływ na wybrane izoformy cytochromu P450 w wątrobie

    Directory of Open Access Journals (Sweden)

    Andrzej Plewka


    Full Text Available Background: In case of overdose of paracetamol the ability of hepatic biotransformation is saturated and accumulation of toxic metabolite – NAPQI takes place. Main CYP isoforms considered to be responsible for bioactivation of APAP and promoting the same liver intoxication are CYP2E1, CYP1A2, CYP3A4 and in animals 2B1/2 isoforms additionally. Purpose of this work was examination of paracetamol influence and/or trichloroethylene on the composition of hepatic cytochrome P450 isoforms. Materials and method: Tests were carried out on rats which were treated with trichloroethylene, paracetamol and/or N-acetylcysteine. In the microsomal fraction content of three isoforms of cytochrome P450 i.e. CYP2E1, CYP2B1/2 and CYP1A2 were determined. Results: Paracetamol slightly stimulated CYP2B1/2 lowering simultaneously level of CYP1A2. Trichloroethylene stimulated CYP2B1/2. N-acetylcysteine stimulated all tested P450 isoforms. N-acetylcysteine given together with examinated xenobiotics induced studied P450 isoforms. Conclusions: N-acetylcysteine demonstrated a protective effect on studied CYP isoforms especially when was given upon termination of xenobiotics exposure

  7. Acetaminophen overdose associated with double serum concentration peaks

    Directory of Open Access Journals (Sweden)

    Cristian Papazoglu


    Full Text Available Acetaminophen is the most commonly used analgesic–antipyretic medication in the United States. Acetaminophen overdose, a frequent cause of drug toxicity, has been recognized as the leading cause of fatal and non-fatal hepatic necrosis. N-Acetylcysteine is the recommended antidote for acetaminophen poisoning. Despite evidence on the efficacy of N-acetylcysteine for prevention of hepatic injury, controversy persists about the optimal duration of the therapy. Here, we describe the case of a 65-year-old male with acetaminophen overdose and opioid co-ingestion who developed a second peak in acetaminophen serum levels after completing the recommended 21-hour intravenous N-acetylcysteine protocol and when the standard criteria for monitoring drug levels was achieved. Prolongation of N-acetylcysteine infusion beyond the standard protocol, despite a significant gap in treatment, was critical for successful avoidance of hepatotoxicity. Delay in acetaminophen absorption may be associated with a second peak in serum concentration following an initial declining trend, especially in cases of concomitant ingestion of opioids. In patients with acetaminophen toxicity who co-ingest other medications that may potentially delay gastric emptying or in those with risk factors for delayed absorption of acetaminophen, we recommend close monitoring of aminotransferase enzyme levels, as well as trending acetaminophen concentrations until undetectable before discontinuing the antidote therapy.

  8. Oxidative Hemolysis of Erythrocytes (United States)

    Wlodek, Lidia; Kusior, Dorota


    This exercise for students will allow them to simultaneously observe lipid peroxidation and consequent hemolysis of rat erythrocytes and the effect of sodium azide, a catalase inhibitor, on these processes. It will also demonstrate a protective action of antioxidants, the therapeutically used N-acetylcysteine and albumins present in plasma.

  9. Hyperactivity of the Ero1α Oxidase Elicits Endoplasmic Reticulum Stress but No Broad Antioxidant Response

    DEFF Research Database (Denmark)

    Hansen, Henning Gram; Schmidt, Jonas Damgard; Soltoft, Cecilie Lutzen


    and induces expression of two established unfolded protein response (UPR) targets, BiP (immunoglobulin-binding protein) and HERP (homocysteine-induced ER protein). These effects could be reverted or aggravated by N-acetylcysteine and buthionine sulfoximine, respectively. Because both agents manipulate...

  10. Physical changes in bovine lens homogenate following ultraviolet irradiation and their prevention by some compounds

    International Nuclear Information System (INIS)

    Ohmori, Shinji; Nose, Hideko


    Exposure of the dialyzed supernatant of bovine lens homogenate to ultraviolet (UV) light led to increases in its turbidity, pigmentation, and viscosity. These photochemically induced alterations of lens proteins were prevented by glutathione, cysteine and N-acetylcysteine, but not by ascorbic acid, S-(1,2-dicarboxyethyl)-glutathione or dulcitol. (author)

  11. Antioxidant treatment ameliorates experimental diabetes-induced depressive-like behaviour and reduces oxidative stress in brain and pancreas. (United States)

    Réus, Gislaine Z; Dos Santos, Maria Augusta B; Abelaira, Helena M; Titus, Stephanie E; Carlessi, Anelise S; Matias, Beatriz I; Bruchchen, Livia; Florentino, Drielly; Vieira, Andriele; Petronilho, Fabricia; Ceretta, Luciane B; Zugno, Alexandra I; Quevedo, João


    Studies have shown a relationship between diabetes mellitus (DM) and the development of major depressive disorder. Alterations in oxidative stress are associated with the pathophysiology of both diabetes mellitus and major depressive disorder. This study aimed to evaluate the effects of antioxidants N-acetylcysteine and deferoxamine on behaviour and oxidative stress parameters in diabetic rats. To this aim, after induction of diabetes by a single dose of alloxan, Wistar rats were treated with N-acetylcysteine or deferoxamine for 14 days, and then depressive-like behaviour was evaluated. Oxidative stress parameters were assessed in the prefrontal cortex, hippocampus, amygdala, nucleus accumbens and pancreas. Diabetic rats displayed depressive-like behaviour, and treatment with N-acetylcysteine reversed this alteration. Carbonyl protein levels were increased in the prefrontal cortex, hippocampus and pancreas of diabetic rats, and both N-acetylcysteine and deferoxamine reversed these alterations. Lipid damage was increased in the prefrontal cortex, hippocampus, amygdala and pancreas; however, treatment with N-acetylcysteine or deferoxamine reversed lipid damage only in the hippocampus and pancreas. Superoxide dismutase activity was decreased in the amygdala, nucleus accumbens and pancreas of diabetic rats. In diabetic rats, there was a decrease in catalase enzyme activity in the prefrontal cortex, amygdala, nucleus accumbens and pancreas, but an increase in the hippocampus. Treatment with antioxidants did not have an effect on the activity of antioxidant enzymes. In conclusion, animal model of diabetes produced depressive-like behaviour and oxidative stress in the brain and periphery. Treatment with antioxidants could be a viable alternative to treat behavioural and biochemical alterations induced by diabetes. Copyright © 2015 John Wiley & Sons, Ltd.

  12. Serotonergic neurotoxic metabolites of ecstasy identified in rat brain. (United States)

    Jones, Douglas C; Duvauchelle, Christine; Ikegami, Aiko; Olsen, Christopher M; Lau, Serrine S; de la Torre, Rafael; Monks, Terrence J


    The selective serotonergic neurotoxicity of 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) depends on their systemic metabolism. We have recently shown that inhibition of brain endothelial cell gamma-glutamyl transpeptidase (gamma-GT) potentiates the neurotoxicity of both MDMA and MDA, indicating that metabolites that are substrates for this enzyme contribute to the neurotoxicity. Consistent with this view, glutathione (GSH) and N-acetylcysteine conjugates of alpha-methyl dopamine (alpha-MeDA) are selective neurotoxicants. However, neurotoxic metabolites of MDMA or MDA have yet to be identified in brain. Using in vivo microdialysis coupled to liquid chromatography-tandem mass spectroscopy and a high-performance liquid chromatography-coulometric electrode array system, we now show that GSH and N-acetylcysteine conjugates of N-methyl-alpha-MeDA are present in the striatum of rats administered MDMA by subcutaneous injection. Moreover, inhibition of gamma-GT with acivicin increases the concentration of GSH and N-acetylcysteine conjugates of N-methyl-alpha-MeDA in brain dialysate, and there is a direct correlation between the concentrations of metabolites in dialysate and the extent of neurotoxicity, measured by decreases in serotonin (5-HT) and 5-hydroxyindole acetic (5-HIAA) levels. Importantly, the effects of acivicin are independent of MDMA-induced hyperthermia, since acivicin-mediated potentiation of MDMA neurotoxicity occurs in the context of acivicin-mediated decreases in body temperature. Finally, we have synthesized 5-(N-acetylcystein-S-yl)-N-methyl-alpha-MeDA and established that it is a relatively potent serotonergic neurotoxicant. Together, the data support the contention that MDMA-mediated serotonergic neurotoxicity is mediated by the systemic formation of GSH and N-acetylcysteine conjugates of N-methyl-alpha-MeDA (and alpha-MeDA). The mechanisms by which such metabolites access the brain and produce selective

  13. Method for increasing the yield of mined coal subjected to foam flotation. Verfahren zur Erhoehung der Ausbeute von unter Tage abgebauter Kohle, die einer Konzentration durch Schaumflotation unterworfen wird

    Energy Technology Data Exchange (ETDEWEB)

    Finch, R.E.


    The foam flotation of coal mined underground gives better yields if one uses a water in oil emulsion of sodium polyacrytate. It is best to use 0.007 to 0.227 kg of dry sodium polyacrylate on a tonne of dry coal. The use of an aliphatic hydrocarbon and a stabiliser for the emulsion is preferred. Several examples are given, which enable one to judge the results.

  14. Die Integration von Tablet-Computern in das Medizinstudium. Teil 1: Eine Umfragestudie unter den Studierenden der Medizinischen Fakultät der Universität Münster

    Directory of Open Access Journals (Sweden)

    Becker, Jan C.


    Full Text Available Students of medicine frequently make use of tablet computers for educational purposes. So the landscape of digital mobile learning behavior should be explored in depth. Moreover the faculty wants to know their students’ needs to give them more support. This study among students of the medical faculty of the University of Münster examined the way they use smartphones and tablet computers as well as their expectations regarding lectures, the organization of courses and the communication with teachers. 942 students of medicine and dentistry took part in this online survey. 35% owned a tablet and a further 9% planned to buy one. 62% of the students stated that tablets could be