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Sample records for zum bescheid nr

  1. Handbuch der Forschung zum Lehrerberuf

    CERN Document Server

    Bennewitz, Hedda; Rothland, Martin

    2014-01-01

    Die große Bedeutung, die dem Lehrerberuf in der Öffentlichkeit, aber auch in den Bildungswissenschaften für die Qualität von Schule und Unterricht zugemessen wird, stand bislang in deutlichem Kontrast zu Umfang und Intensität der Forschung, die auf den Lehrerberuf gerichtet war. Diese immer wieder beklagte Situation hat sich jedoch gewandelt. Seit etwa einem Jahrzehnt ist eine deutliche Intensivierung und allmähliche Verstetigung der Forschung zum Lehrerberuf zu beobachten. Mit dem vorliegenden Handbuch wird erstmalig eine repräsentative Übersicht über den aktuellen Stand der Forschung zum Lehrerberuf vermittelt. Unter intensiver Berücksichtigung der internationalen Fachdiskussion informieren die 45 Beiträge des Handbuchs über Forschung zu folgenden Themenfeldern: Geschichte des Lehrerberufs, Charakteristika und Rahmenbedingungen des Lehrerberufs, Konzepte der Forschung zum Lehrerberuf, Berufsbiographien von Lehrerinnen und Lehrern, Lehrerbildung, Kognitionen, Emotionen und Kompetenzen von Lehrern...

  2. Illicit operation of industrial plant subject to licensing, or of other installations within the purview of the German Federal Emission Control Act (BImSchG), which have been shut down for protection against hazards (section 327, subsection 2, No. 1 Penal Code (StGB)); Das unerlaubte Betreiben von genehmigungsbeduerftigen Anlagen oder sonstigen Anlagen im Sinne des Bundes-Immissionsschutzgesetzes, deren Betrieb zum Schutz vor Gefahren untersagt worden ist (Paragraph 327 Abs.2 Nr.1 StGB)

    Energy Technology Data Exchange (ETDEWEB)

    Ocker, A.

    1995-12-31

    verwaltungsrechtlichen Fehlern auf das Strafrecht. Daneben werden die spezifischen Problempunkte des Para. 327 Abs. 2 Nr. 1 StGB beachtet, die eine starke verwaltungsrechtliche Auspraegung aufweisen. Hervorzuheben sind hier insbesondere die Ausfuehrungen zum Anlagenbegriff i.S.d. BImSchG. (orig./HSCH)

  3. Effects of NR1 splicing on NR1/NR3B-type excitatory glycine receptors

    Directory of Open Access Journals (Sweden)

    Orth Angela

    2009-04-01

    Full Text Available Abstract Background N-methyl-D-aspartate receptors (NMDARs are the most complex of ionotropic glutamate receptors (iGluRs. Subunits of this subfamily assemble into heteromers, which – depending on the subunit combination – may display very different pharmacological and electrophysiological properties. The least studied members of the NMDAR family, the NR3 subunits, have been reported to assemble with NR1 to form excitatory glycine receptors in heterologous expression systems. The heterogeneity of NMDARs in vivo is in part conferred to the receptors by splicing of the NR1 subunit, especially with regard to proton sensitivity. Results Here, we have investigated whether the NR3B subunit is capable of assembly with each of the eight functional NR1 splice variants, and whether the resulting receptors share the unique functional properties described for NR1-1a/NR3. We provide evidence that functional excitatory glycine receptors formed regardless of the NR1 isoform, and their pharmacological profile matched the one reported for NR1-1a/NR3: glycine alone fully activated the receptors, which were insensitive to glutamate and block by Mg2+. Surprisingly, amplitudes of agonist-induced currents showed little dependency on the C-terminally spliced NR1 variants in NR1/NR3B diheteromers. Even more strikingly, NR3B conferred proton sensitivity also to receptors containing NR1b variants – possibly via disturbing the "proton shield" of NR1b splice variants. Conclusion While functional assembly could be demonstrated for all combinations, not all of the specific interactions seen for NR1 isoforms with coexpressed NR2 subunits could be corroborated for NR1 assembly with NR3. Rather, NR3 abates trafficking effects mediated by the NR1 C terminus as well as the N-terminally mediated proton insensitivity. Thus, this study establishes that NR3B overrides important NR1 splice variant-specific receptor properties in NR1/NR3B excitatory glycine receptors.

  4. RANK und RANKL - Vom Knochen zum Mammakarzinom

    Directory of Open Access Journals (Sweden)

    Sigl V

    2012-01-01

    Full Text Available RANK („Receptor Activator of NF-κB“ und sein Ligand RANKL sind Schlüsselmoleküle im Knochenmetabolismus und spielen eine essenzielle Rolle in der Entstehung von pathologischen Knochenveränderungen. Die Deregulation des RANK/RANKL-Systems ist zum Beispiel ein Hauptgrund für das Auftreten von postmenopausaler Osteoporose bei Frauen. Eine weitere wesentliche Funktion von RANK und RANKL liegt in der Entwicklung von milchsekretierenden Drüsen während der Schwangerschaft. Dabei regulieren Sexualhormone, wie zum Beispiel Progesteron, die Expression von RANKL und induzieren dadurch die Proliferation von epithelialen Zellen der Brust. Seit Längerem war schon bekannt, dass RANK und RANKL in der Metastasenbildung von Brustkrebszellen im Knochengewebe beteiligt sind. Wir konnten nun das RANK/RANKLSystem auch als essenziellen Mechanismus in der Entstehung von hormonellem Brustkrebs identifizieren. In diesem Beitrag werden wir daher den neuesten Erkenntnissen besondere Aufmerksamkeit schenken und diese kritisch in Bezug auf Brustkrebsentwicklung betrachten.

  5. Kurz zum Klima: Zu viel Salz verdirbt den Boden

    OpenAIRE

    Zimmer, Markus; Lippelt, Jana; Frank, Jonas

    2012-01-01

    Der zunehmende Einsatz von Anlagen zur Bewässerung sowie mangelnde Erfahrungswerte und Unkenntnis über den richtigen Einsatz der künstlichen Bewässerung haben in den letzten Jahrzehnten zu einer massiven Zunahme der Versalzung des Bodens geführt. Durch diese Bodenversalzung werden weite Flächen für die Landwirtschaft unbrauchbar. Sie sind nur durch hohen Aufwand und Kosten wieder renaturierbar. Der Beitrag in der Reihe »Kurz zum Klima« gibt einen Überblick über die Versalzung in Anbaugebiet...

  6. Prokofieff: Kantate zum 20. Jahrestag der Oktoberrevolution, Op.74, Neeme Järvi / Joachim Salau

    Index Scriptorium Estoniae

    Salau, Joachim

    1993-01-01

    Uuest heliplaadist "Prokofieff: Kantate zum 20. Jahrestag der Oktoberrevolution, Op.74, Auszüge aus Das Märchen von der steinernen Blume. Gennadij Roshdestwenskij (Sprecher), Philharmonia Chorus, Philharmonia Orchestra, Neeme Järvi. Chandos/Koch CD 9095

  7. [Karin Hallas. Das Tallinner Mietshaus. Vom Historismus bis zum Jugendstil] / Paul Kaegbein

    Index Scriptorium Estoniae

    Kaegbein, Paul

    2006-01-01

    Arvustus: Karin Hallas. Das Tallinner Mietshaus. Vom Historismus bis zum Jugendstil. In: Architektur und bildende Kunst im Baltikum um 1900. Frankfurt am Main, Berlin, Bern, Bruxelles, New York, Wien: Lang 1999. S. 173-192.

  8. Das migrationspolitische Feld der Schweiz: eine politikwissenschaftliche Analyse der Vernehmlassung zum Arbenzbericht

    OpenAIRE

    Mahnig, Hans

    2007-01-01

    Der Bericht, vom Bundesamt für Flüchtlinge (BFF) in Auftrag gegeben, stellt eine politikwissenschaftliche Analyse der Vernehmlassung zum Bericht Arbenz vom Mai 1995 dar, welche parallel zur Auswertung durch das BFF selbst unternommen wurde. Er hat zum Ziel, die zentralen Konflikt- und Konsensfelder in der gegenwärtigen migrationspolitischen Auseinandersetzung der Schweiz herauszuarbeiten. Die wichtigsten Resultate der Analyse sind die folgenden: Die Internationale Flüchtlingspolitik und die A...

  9. KidSmart - Medienkompetent zum Schulübergang

    Directory of Open Access Journals (Sweden)

    Anita Müller

    2012-03-01

    Full Text Available In Deutschland zeichnet sich bereits vor Schuleintritt im Bereich der literarischen und medialen Sozialisation eine Bildungsbenachteiligung ab. Vor allem Kinder aus bildungsfernen Milieus und mit Migrationshintergrund sind hiervon betroffen (vgl. Becker/Lauterbach 2004; Diefenbach 2007; Rabe-Kleberg 2010. An dieser Einsicht knüpft das Forschungs- und Interventionsprojekt KidSmart – Medienkompetent zum Schulübergang an. Medienpädagogische Arbeit steht hier im Kontext der Vorbereitung auf den Schulübergang. Durch kreative Medienarbeit soll ein Gegengewicht zur eher passiven Mediennutzung geschaffen werden. Ziel ist es Bildungsunterschiede von Kindern bereits vor Schulbeginn auszugleichen.Already before starting school educational disadvantages in the part of reading and media socialization can be identified within Germany. Generally affected are children of less educated families and children with migration background (Becker/Lauterbach 2004; Diefenbach 2007; Rabe-Kleberg 2010. Therefore, an exceptional intervention and research project KidSmart – media competence by entering school was initiated in the area of early childhood education. Within this project media-pedagogical work is corresponding with preparation of entering school. The intention is to create a counterbalance to passive media use by creative media work. The scope of the project is to fight against early educational disadvantages.

  10. Landeskundeunterricht zum Nationalsozialismus. Ein Praxisbericht aus der Mongolei

    Directory of Open Access Journals (Sweden)

    Ralf Heimrath

    2015-03-01

    Full Text Available In einer Umfrage bei den fortgeschrittenen Studierenden an der Deutschen Abteilung der Nationaluniversität der Mo n- golei wurde festgestellt, dass der Name Hitler sehr bekannt und mit e iner positiven Konnotation verbunden ist. Dagegen gab es keine Kenntnisse über die Zeit des Nationalsozialismus in Deutschland. Mehrere weitere Beobachtungen lassen darüber hinaus in der Mongolei und in anderen Ländern eine bestimmte Hitler - Verehrung erken nen. Vor diesem Hintergrund wurde im Jahr 2012 an der Deutschen Abteilung der Nationaluniversität der Mongolei eine Unterricht s- sequenz zum Thema Nationalsozialismus in Deutschland durchgeführt. Die wichtigsten Themen sind dabei die Persönlichkeit des Dikt ators, der Werdegang der NSDAP während der Weimarer Republik und das Schicksal der jüdischen Bevölkerung in Deutschland vor und insbesondere nach der Machtergreifung Hitlers. Die Sequenz dient der Relativierung des Hitler - Bildes im Bewusstsein der Studiere nden und der Vermittlung von Kenntnissen zur Geschichte des 20. Jahrhunderts als Grundlage einer Entwicklung, die zu zwei deutschen Staaten und ihrer späteren Vereinigung führte. Die Sequenz ist auf vergleichbare Unterrichtssituationen in anderen Ländern übertragbar.

  11. Teilchen, Felder, Quanten von der Quantenmechanik zum Standardmodell der Teilchenphysik

    CERN Document Server

    Ecker, Gerhard

    2017-01-01

    Dieses Buch bietet Ihnen eine Einführung in den aktuellen Stand unseres Wissens über die Struktur der Materie. Gerhard Ecker beschreibt verständlich die Entwicklung der modernen Physik vom Beginn des Quantenzeital­ters bis zum Standardmodell der Teilchenphysik, der umfassenden Theorie der fundamentalen Wechselwir­kungen des Mikrokosmos. Dabei wird der Schwerpunkt auf die wichtigsten Entdeckungen und Entwicklungen, beispielsweise der Quantenfeldtheorie, der Eichtheorien und die Zukunft der Teilchenphysik, gelegt. Besonders hebt der Autor auch das Wechselspiel zwischen Theorie und Experiment hervor, die uns helfen, die tiefsten Rätsel der Natur zu ergründen. Teilchen, Felder, Quanten ist für alle geschrieben, die Freude an der Physik haben. Es bietet Abitu­rienten und Studierenden der Physik in den ersten Semestern einen Ansporn, die Physik tiefer zu verstehen. Lehrer und andere an der Phy­sik Interessierte werden darin nützliche Einblicke in die Welt der Teilchenphysik finden. Für Studierende in ...

  12. Mis toimub "Objektis nr 2011"? / Elli Kalju

    Index Scriptorium Estoniae

    Kalju, Elli

    2011-01-01

    Tallinnas Vabaduse väljakul 27.02.-20.03.2011 paiknevast installatsioonist "Objekt nr. 2011", kus alati keegi sees istub. Kultuuritegelased vahetuvad iga tunni järel, järjekorda oodatakse Tallinna Kunsihoones asuvas staabis

  13. Flamenkofantaasia vallutused. Urmas Sisaski Missa nr 5

    Index Scriptorium Estoniae

    2008-01-01

    Kontserdist "Flamenkofantaasiad" 2. mail Jõhvi kontserdimajas, 3. mail Tallinna Raekojas ja 4. mail Vanemuise kontserdimajas. Eesti Vabariigi 90 a juubeli auks loodud Urmas Sisaski Missa nr 5 "Chateau Rocher" esitusest 2. ja 3. mail Tallinnas ja Tartus

  14. Cross-talk between the NR3B and NR4A families of orphan nuclear receptors

    International Nuclear Information System (INIS)

    Lammi, Johanna; Rajalin, Ann-Marie; Huppunen, Johanna; Aarnisalo, Piia

    2007-01-01

    Estrogen-related receptors (NR3B family) and Nurr1, NGFI-B, and Nor1 (NR4A family) are orphan nuclear receptors lacking identified natural ligands. The mechanisms regulating their transcriptional activities have remained elusive. We have previously observed that the members of NR3B and NR4A families are coexpressed in certain cell types such as osteoblasts and that the ability of Nurr1 to transactivate the osteopontin promoter is repressed by ERRs. We have now studied the cross-talk between NR3B and NR4A receptors. We show that NR3B and NR4A receptors mutually repress each others' transcriptional activity. The repression involves intact DNA-binding domains and dimerization interfaces but does not result from competition for DNA binding or from heterodimerization. The activation functions of NR3B and NR4A receptors are dispensable for the cross-talk. In conclusion, we report that cross-talk between NR3B and NR4A receptors is a mechanism modulating the transcriptional activities of these orphan nuclear receptors

  15. NR-code: Nonlinear reconstruction code

    Science.gov (United States)

    Yu, Yu; Pen, Ue-Li; Zhu, Hong-Ming

    2018-04-01

    NR-code applies nonlinear reconstruction to the dark matter density field in redshift space and solves for the nonlinear mapping from the initial Lagrangian positions to the final redshift space positions; this reverses the large-scale bulk flows and improves the precision measurement of the baryon acoustic oscillations (BAO) scale.

  16. Analysis list: NR3C1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NR3C1 Blood,Bone,Breast,Liver,Others,Prostate,Uterus + hg19 http://dbarchive.biosci...encedbc.jp/kyushu-u/hg19/target/NR3C1.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/NR3C1.5.tsv http:...//dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/NR3C1.10.tsv http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/colo/NR3C1.Blood.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/NR3C1.Bone.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/NR3C1.Breast.tsv,http://dbarchive.bi

  17. Analysis list: Nr3c1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Nr3c1 Adipocyte,Blood,Breast,Embryo,Embryonic fibroblast,Liver,Neural + mm9 http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/target/Nr3c1.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/ta...rget/Nr3c1.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Nr3c1.10.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/colo/Nr3c1.Adipocyte.tsv,http://dbarchive.biosciencedbc.jp.../kyushu-u/mm9/colo/Nr3c1.Blood.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Nr3c1.Breast.tsv,http://dbarchive.bioscience

  18. Differential Roles for "Nr4a1" and "Nr4a2" in Object Location vs. Object Recognition Long-Term Memory

    Science.gov (United States)

    McNulty, Susan E.; Barrett, Ruth M.; Vogel-Ciernia, Annie; Malvaez, Melissa; Hernandez, Nicole; Davatolhagh, M. Felicia; Matheos, Dina P.; Schiffman, Aaron; Wood, Marcelo A.

    2012-01-01

    "Nr4a1" and "Nr4a2" are transcription factors and immediate early genes belonging to the nuclear receptor Nr4a family. In this study, we examine their role in long-term memory formation for object location and object recognition. Using siRNA to block expression of either "Nr4a1" or "Nr4a2", we found that "Nr4a2" is necessary for both long-term…

  19. Potentiation of glycine-gated NR1/NR3A NMDA receptors relieves Ca2+-dependent outward rectification

    Directory of Open Access Journals (Sweden)

    Christian Madry

    2010-03-01

    Full Text Available Glycine has diverse functions within the mammalian central nervous system. It inhibits postsynaptic neurons via strychnine-sensitive glycine receptors (GlyRs and enhances neuronal excitation through co-activation of N-methyl-D-aspartate (NMDA receptors. Classical Ca2+-permeable NMDA receptors are composed of glycine-binding NR1 and glutamate-binding NR2 subunits, and hence require both glutamate and glycine for efficient activation. In contrast, recombinant receptors composed of NR1 and the glycine binding NR3A and/or NR3B subunits lack glutamate binding sites and can be activated by glycine alone. Therefore these receptors are also named excitatory glycine receptors. Co-application of antagonists of the NR1 glycine-binding site or of the divalent cation Zn2+ markedly enhances the glycine responses of these receptors. To gain further insight into the properties of these glycine-gated NMDA receptors, we investigated their current-voltage (I-V dependence. Whole-cell current-voltage relations of glycine currents recorded from NR1/NR3B and NR1/NR3A/NR3B expressing oocytes were found to be linear under our recording conditions. In contrast, NR1/NR3A receptors displayed a strong outwardly rectifying I-V relation. Interestingly, the voltage-dependent inward current block was abolished in the presence of NR1 antagonists, Zn2+ or a combination of both. Further analysis revealed that Ca2+ (1.8 mM present in our recording solutions was responsible for the voltage-dependent inhibition of ion flux through NR1/NR3A receptors. Since physiological concentrations of the divalent cation Mg2+ did not affect the I-V dependence, our data suggest that relief of the voltage-dependent Ca2+ block of NR1/NR3A receptors by Zn2+ may be important for the regulation of excitatory glycinergic transmission, according to the Mg2+-block of conventional NR1/NR2 NMDA receptors.

  20. Zum Entwicklungsstand und zu Entwicklungsproblemen unserer Jugend - 1987 : Forschungsergebnisse - Beobachtungen - Überlegungen in 10 Thesen

    OpenAIRE

    Friedrich, Walter

    1987-01-01

    Dieser Bericht gibt in zehn Thesen einen Überblick über Forschungsergebnisse, Beobachtungen und Überlegungen zum Entwicklungsstand der DDR-Jugend im Jahre 1987. Diese sei "in ihrer großen Mehrheit durch ein hohes Niveau ihrer sozialistischen Persönlichkeitsentwicklung charakterisiert". Kritisiert wird die Rolle der FDJ, die die Interessenvertretung der Jugend nicht ausreichend wahrnehme. Weiterhin nehme die Rezeption westlicher Rundfunk- und Fernsehsendungen immer mehr zu, jedoch sei es verbo...

  1. Pädagogik als Kulturwissenschaft. Programmatische Überlegungen zum Status der Allgemeinen Erziehungswissenschaft

    OpenAIRE

    Wimmer, Michael

    2002-01-01

    Ausgehend von einer schlaglichtartigen Charakterisierung gegenwärtiger Transformationsprozesse, die nicht nur zu grundlegenden Wandlungen in den gesellschaftlichen Verhältnissen und im kulturellen Selbstverständnis geführt haben, sondern auch die Prämissen ihrer begrifflichen Identifizierbarkeit und einer reflexiven Selbstvergewisserung erschüttern, wird die interne Situation der Erziehungswissenschaft skizziert, die sich ebenfalls dilemmatisch darstellt. Denn zum einen haben neuere Theorieen...

  2. Wie man Elementarteilchen entdeckt vom Zyklotron zum LHC : ein Streifzug durch die Welt der Teilchenbeschleuniger

    CERN Document Server

    Freytag, Carl

    2016-01-01

    Dieses Buch erklärt die physikalischen Grundlagen und die Technologien der Elementarteilchenforschung und beschreibt allgemeinverständlich Teilchenbeschleuniger und -detektoren sowie ihr Zusammenspiel. An einigen Meilensteinen der Forschung – von der Erzeugung von Transuranen über die Entdeckung exotischer Mesonen bis zum Higgs-Boson – zeigen die Autoren den Weg von der Theorie über das Experiment zum Forschungsergebnis auf. Gravitonen, Higgs-Teilchen, Neutrinos und Quarks – das Interesse an den kleinsten uns bekannten Teilchen ist seit Jahrzehnten ungebrochen und rückt damit auch die Laboratorien in den Blick, die an die Grenzen der Physik vorstoßen: Neben dem größten Experimentierfeld, das wir haben – dem Universum selbst - sind es die gigantischen Maschinen der Elementarteilchenphysik in Großforschungseinrichtungen wie dem CERN und dem DESY. Mit ihnen versuchen Forscher weltweit unter Einsatz extrem hoher Energien Zustände zu simulieren, wie sie zum Beginn unseres Universums kurz nach d...

  3. Analysis list: NR1H3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NR1H3 Adipocyte,Blood + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target.../NR1H3.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/NR1H3.5.tsv http://dbarchive.bioscienced...bc.jp/kyushu-u/hg19/target/NR1H3.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/NR1H3.Adipocyte....tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/NR1H3.Blood.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/hg19/colo/Adipocyte.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Blood.gml ...

  4. The nuclear receptor NR2E1/TLX controls senescence

    Science.gov (United States)

    Krusche, Benjamin; Pemberton, Helen; Alonso, Marta M.; Chandler, Hollie; Brookes, Sharon; Parrinello, Simona; Peters, Gordon; Gil, Jesús

    2014-01-01

    The nuclear receptor NR2E1 (also known as TLX or tailless) controls the self-renewal of neural stem cells (NSCs) and has been implied as an oncogene which initiates brain tumours including glioblastomas. Despite NR2E1 regulating targets like p21CIP1 or PTEN we still lack a full explanation for its role in NSC self-renewal and tumorigenesis. We know that Polycomb repressive complexes (PRC) also control stem cell self-renewal and tumorigenesis, but so far, no formal connection has been established between NR2E1 and PRCs. In a screen for transcription factors regulating the expression of the Polycomb protein CBX7, we identified NR2E1 as one of its more prominent regulators. NR2E1 binds at the CBX7 promoter, inducing its expression. Notably CBX7 represses NR2E1 as part of a regulatory loop. Ectopic NR2E1 expression inhibits cellular senescence, extending cellular lifespan in fibroblasts via CBX7-mediated regulation of p16INK4a and direct repression of p21CIP1. In addition NR2E1 expression also counteracts oncogene-induced senescence (OIS). The importance of NR2E1 to restrain senescence is highlighted through the process of knocking down its expression, which causes premature senescence in human fibroblasts and epithelial cells. We also confirmed that NR2E1 regulates CBX7 and restrains senescence in NSCs. Finally, we observed that the expression of NR2E1 directly correlates with that of CBX7 in human glioblastoma multiforme. Overall we identified control of senescence and regulation of Polycomb action as two possible mechanisms that can join those so far invoked to explain the role of NR2E1 in control of NSC self-renewal and cancer. PMID:25328137

  5. The nuclear receptor NR2E1/TLX controls senescence.

    Science.gov (United States)

    O'Loghlen, Ana; Martin, Nadine; Krusche, Benjamin; Pemberton, Helen; Alonso, Marta M; Chandler, Hollie; Brookes, Sharon; Parrinello, Simona; Peters, Gordon; Gil, Jesús

    2015-07-30

    The nuclear receptor NR2E1 (also known as TLX or tailless) controls the self-renewal of neural stem cells (NSCs) and has been implied as an oncogene which initiates brain tumors including glioblastomas. Despite NR2E1 regulating targets like p21(CIP1) or PTEN we still lack a full explanation for its role in NSC self-renewal and tumorigenesis. We know that polycomb repressive complexes also control stem cell self-renewal and tumorigenesis, but so far, no formal connection has been established between NR2E1 and PRCs. In a screen for transcription factors regulating the expression of the polycomb protein CBX7, we identified NR2E1 as one of its more prominent regulators. NR2E1 binds at the CBX7 promoter, inducing its expression. Notably CBX7 represses NR2E1 as part of a regulatory loop. Ectopic NR2E1 expression inhibits cellular senescence, extending cellular lifespan in fibroblasts via CBX7-mediated regulation of p16(INK4a) and direct repression of p21(CIP1). In addition NR2E1 expression also counteracts oncogene-induced senescence. The importance of NR2E1 to restrain senescence is highlighted through the process of knocking down its expression, which causes premature senescence in human fibroblasts and epithelial cells. We also confirmed that NR2E1 regulates CBX7 and restrains senescence in NSCs. Finally, we observed that the expression of NR2E1 directly correlates with that of CBX7 in human glioblastoma multiforme. Overall we identified control of senescence and regulation of polycomb action as two possible mechanisms that can join those so far invoked to explain the role of NR2E1 in control of NSC self-renewal and cancer.

  6. PROPERTIES OF NR AND NR/ENR BASED RUBBER COMPOUNDS REINFORCED WITH CHOPPED AND SIZED CARBON FIBER

    Directory of Open Access Journals (Sweden)

    Bağdagül Karaağaç

    2016-12-01

    Full Text Available High elasticity, mechanical resistance and antivibration characteristics of natural rubber (NR are essential issue in the main area of vehicle tyres and high modulus demanding bearing applications. In this study, especially in bearing applications, where natural rubber modulus properties are limited, natural rubber has been reinforced with chopped and hydrocarbon sized carbon fiber to get improved tensile modulus. Besides, epoxidized natural rubber (ENR, which was produced by chemical modification of natural rubber, blended with NR and the compounds have been reinforced with epoxy sized carbon fiber. NR and NR/ENR based rubber compounds’ rheological, mechanical, and aging properties have been systematically investigated and evaluated.

  7. Zum Erleben von Thrills und anderen starken emotionalen Reaktionen beim Musikhören

    OpenAIRE

    Schönberger, Jörg

    2003-01-01

    Obwohl unbestritten ist, dass Musik in der Lage ist, starke emotionale Reaktionen auszulösen, hat sich die Musikpsychologie wenig mit den Emotionen beschäftigt, die Menschen beim Musikhören erleben. Zwei mögliche Zugänge zum Erfassen solch starker emotionaler Reaktionen sind die Ansätze von John A. Sloboda und Alf Gabrielsson. Sloboda nutzt für seinen Ansatz das Phänomen der "Thrills" — physiologische Begleiterscheinungen starker emotionaler Reaktionen wie z.B. Gänsehaut, Schauer, die ü...

  8. NR4A orphan nuclear receptor family members, NR4A2 and NR4A3, regulate neutrophil number and survival.

    Science.gov (United States)

    Prince, Lynne R; Prosseda, Svenja D; Higgins, Kathryn; Carlring, Jennifer; Prestwich, Elizabeth C; Ogryzko, Nikolay V; Rahman, Atiqur; Basran, Alexander; Falciani, Francesco; Taylor, Philip; Renshaw, Stephen A; Whyte, Moira K B; Sabroe, Ian

    2017-08-24

    The lifespan of neutrophils is plastic and highly responsive to factors that regulate cellular survival. Defects in neutrophil number and survival are common to both hematologic disorders and chronic inflammatory diseases. At sites of inflammation, neutrophils respond to multiple signals that activate protein kinase A (PKA) signaling, which positively regulates neutrophil survival. The aim of this study was to define transcriptional responses to PKA activation and to delineate the roles of these factors in neutrophil function and survival. In human neutrophil gene array studies, we show that PKA activation upregulates a significant number of apoptosis-related genes, the most highly regulated of these being NR4A2 and NR4A3 Direct PKA activation by the site-selective PKA agonist pair N6/8-AHA (8-AHA-cAMP and N6-MB-cAMP) and treatment with endogenous activators of PKA, including adenosine and prostaglandin E2, results in a profound delay of neutrophil apoptosis and concomitant upregulation of NR4A2/3 in a PKA-dependent manner. NR4A3 expression is also increased at sites of neutrophilic inflammation in a human model of intradermal inflammation. PKA activation also promotes survival of murine neutrophil progenitor cells, and small interfering RNA to NR4A2 decreases neutrophil production in this model. Antisense knockdown of NR4A2 and NR4A3 homologs in zebrafish larvae significantly reduces the absolute neutrophil number without affecting cellular migration. In summary, we show that NR4A2 and NR4A3 are components of a downstream transcriptional response to PKA activation in the neutrophil, and that they positively regulate neutrophil survival and homeostasis. © 2017 by The American Society of Hematology.

  9. Attraction of Euwallacea nr. fornicatus to lures containing quercivorol

    Science.gov (United States)

    Euwallacea nr. fornicatus is an exotic ambrosia beetle that vectors fungal Fusarium spp. to avocados. Two field trials testing potential attractants to trap Euwallacea spp. were conducted in south Florida. Quercivorol + Ultra High Release Ethanol (URH) was the more powerful attractant for E. nr. for...

  10. NR4A nuclear receptors are orphans but not lonesome

    NARCIS (Netherlands)

    Kurakula, Kondababu; Koenis, Duco S.; van Tiel, Claudia M.; de Vries, Carlie J. M.

    2014-01-01

    The NR4A subfamily of nuclear receptors consists of three mammalian members: Nur77, Nurr1, and NOR-1. The NR4A receptors are involved in essential physiological processes such as adaptive and innate immune cell differentiation, metabolism and brain function. They act as transcription factors that

  11. RESTAURANT Nr 1 (building 501 - Meyrin site)

    CERN Multimedia

    2003-01-01

    OPENING TIMES in JANUARY-FEBRUARY 2004 Customers are kindly requested to note the modified opening times of restaurant nr. 1 and the adjoining newspaper stand from Monday, January 5 to Sunday February 29, 2004: Kiosquefrom Monday to Friday07:30 - 17:00 hrs Restaurant from Monday to FridaySaturday / Sunday 07:00 - 23:00 hrs08:00 - 21:00 hrs Hot meals will be served between 11:30 and 14:00 hrs, then from 18:00 to 19:30 hrs. Restaurant Supervisory Committee

  12. >RESTAURANT Nr 1 (building 501 - Meyrin site)

    CERN Multimedia

    2004-01-01

    Customers are kindly requested to note the modified opening times of restaurant nr. 1 and the adjoining newspaper stand from Monday, January 5 to Sunday February 29, 2004: - Kiosque from Monday to Friday 07:30 - 17:00 hrs - Restaurant from Monday to Friday Saturday / Sunday 07:00 - 23:00 hrs08:00 - 21:00 hrs Hot meals will be served between 11:30 and 14:00 hrs, then from 18:00 to 19:30 hrs. Restaurant Supervisory Committee

  13. NR4A nuclear receptors are orphans but not lonesome.

    Science.gov (United States)

    Kurakula, Kondababu; Koenis, Duco S; van Tiel, Claudia M; de Vries, Carlie J M

    2014-11-01

    The NR4A subfamily of nuclear receptors consists of three mammalian members: Nur77, Nurr1, and NOR-1. The NR4A receptors are involved in essential physiological processes such as adaptive and innate immune cell differentiation, metabolism and brain function. They act as transcription factors that directly modulate gene expression, but can also form trans-repressive complexes with other transcription factors. In contrast to steroid hormone nuclear receptors such as the estrogen receptor or the glucocorticoid receptor, no ligands have been described for the NR4A receptors. This lack of known ligands might be explained by the structure of the ligand-binding domain of NR4A receptors, which shows an active conformation and a ligand-binding pocket that is filled with bulky amino acid side-chains. Other mechanisms, such as transcriptional control, post-translational modifications and protein-protein interactions therefore seem to be more important in regulating the activity of the NR4A receptors. For Nur77, over 80 interacting proteins (the interactome) have been identified so far, and roughly half of these interactions has been studied in more detail. Although the NR4As show some overlap in interacting proteins, less information is available on the interactome of Nurr1 and NOR-1. Therefore, the present review will describe the current knowledge on the interactomes of all three NR4A nuclear receptors with emphasis on Nur77. Copyright © 2014 Elsevier B.V. All rights reserved.

  14. Cloning and expression of the human N-methyl-D-aspartate receptor subunit NR3A

    DEFF Research Database (Denmark)

    Eriksson, Maria; Nilsson, Anna; Froelich-Fabre, Susanne

    2002-01-01

    Native N-methyl-D-aspartate (NMDA) receptors are heteromeric assemblies of four or five subunits. The NMDA receptor subunits, NR1, NR2A, NR2B, NR2C, and NR2D have been cloned in several species, including man. The NR3A subunit, which in rodents is predominantly expressed during early development......, seems to function by reducing the NMDA receptor response. The human homologue to the rat NR3A, however, had not been cloned. In order to study the functions of the human NR3A (hNR3A), we have cloned and sequenced the hNR3A. It was found to share 88% of the DNA sequence with the rat gene, corresponding...

  15. The USA Nr Inventory: Dominant Sources and Primary Transport Pathways

    Science.gov (United States)

    Sabo, R. D.; Clark, C.; Sobota, D. J.; Compton, J.; Cooter, E. J.; Schwede, D. B.; Bash, J. O.; Rea, A.; Dobrowolski, J. P.

    2016-12-01

    Efforts to mitigate the deleterious effects of excess reactive nitrogen (Nr) on human health and ecosystem goods and service while ensuring food, biofuel, and fiber availability, is one of the most pressing environmental management challenges of this century. Effective management of Nr requires up to date inventories that quantitatively characterize the sources, transport, and transformation of Nr through the environment. The inherent complexity of the nitrogen cycle, however, through multiple exchange points across air, water, and terrestrial media, renders such inventories difficult to compile and manage. Previous Nr Inventories are for 2002 and 2007, and used data sources that have since been improved. Thus, this recent inventory will substantially advance the methodology across many sectors of the inventory (e.g. deposition and biological fixation in crops and natural systems) and create a recent snapshot that is sorely needed for policy planning and trends analysis. Here we use a simple mass balance approach to estimate the input-output budgets for all United States Geologic Survey Hydrologic Unit Code-8 watersheds. We focus on a recent year (i.e. 2012) to update the Nr Inventory, but apply the analytical approach for multiple years where possible to assess trends through time. We also compare various sector estimates using multiple methodologies. Assembling datasets that account for new Nr inputs into watersheds (e.g., atmospheric NOy deposition, food imports, biologic N fixation) and internal fluxes of recycled Nr (e.g., manure, Nr emmissions/volatilization) provide an unprecedented, data driven computation of N flux. Input-output budgets will offer insight into 1) the dominant sources of Nr in a watershed (e.g., food imports, atmospheric N deposition, or fertilizer), 2) the primary loss pathways for Nr (e.g., crop N harvest, volatilization/emissions), and 3) what watersheds are net sources versus sinks of Nr. These insights will provide needed clarity for

  16. 1995 annual 100-NR-2 groundwater summary report

    International Nuclear Information System (INIS)

    Borghese, J.V.; Johnson, V.M.; Walker, L.D.

    1996-09-01

    The 100-NR-2 Operable Unit (OU) is located in the north-central part of the Hanford Site along the southern shoreline of the Columbia River in Richland, Washington. The 100-N Area is bordered by the Columbia River and the 600 Area (the portion of the Hanford Site that surrounds the primary operations areas). The purpose of this document is to provide the 1995 groundwater sampling data for the 100-NR-2 groundwater OU. Also included are the analytical results for sampling rounds 7 and 8 that were conducted during March and September 1995 for 100-NR-2

  17. Budke, Alexandra; Kanwischer, Detlef; Pott, Andreas (Hg.), Internetgeographien : Beobachtungen zum Verhältnis von Internet, Raum und Gesellschaft / [rezensiert von] Helmut Klüter

    OpenAIRE

    Klüter, Helmut

    2009-01-01

    Rezensiertes Werk: Internetgeographien : Beobachtungen zum Verhältnis von Internet, Raum und Gesellschaft / hrsg. von Alexandra Budke ... - Stuttgart : Steiner, 2004. - 192 S. : Ill., graph. Darst., Kt. - (Erdkundliches Wissen ; 136) ISBN 3-515-08506-8

  18. From traditional locomotive engineers to automated train control; Vom triebfahrzeugfuehrergefuehrten Zug zum Fahrautomaten

    Energy Technology Data Exchange (ETDEWEB)

    Hohnecker, E. [Karlsruhe Univ. (T.H.) (Germany). Inst. fuer Strassenbau und Eisenbahnwesen

    2000-07-01

    Profitability and capacity in public guided transport must be increased. One option is automatic, driverless train control. There are many questions surrounding the legal, technical and operational aspects of automated control which need to be answered. These intrinsic aspects of the system will be presented and discussed. The various options leading to automatic train control as well as the necessary technical measures will also be presented. (orig.) [German] Die Wirtschaftlichkeit und die Leistungsfaehigkeit im oeffentlichen spurgefuehrten Verkehr muessen erhoeht werden. Als Loesung bietet sich zukuenftig auch der automatische und fahrerlose Betrieb an. Das Fahren ohne Triebfahrzeugfuehrer wirft jedoch eine Vielzahl von Fragestellungen auf, die sowohl in juristischer, technischer und betrieblicher Hinsicht beantwortet werden muessen. Diese systemimmanenten Aspekte werden dargestellt und diskutiert. Anschliessend werden die Moeglichkeiten auf dem Weg zum Fahrautomaten aufgezeigt und die technischen Massnahmen zur Umsetzung des automatischen Fahrens erlaeutert. (orig.)

  19. Contributions to the workshop `Residual ponds from open pit brown coal mining`; Beitraege zum Workshop ``Braunkohlebergbaurestseen``

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-31

    The contributions to the workshop ``Investigations on flooding in residual ponds from open-pit mining`` deal, inter alia, with the hydrochemical development of flushing waters, the stability of the water quality of residual lakes, especially under the impact of land contamination, the influence of brine receipts by flushed residual lakes, and with the microbiological ecology of residual lakes from mining. Furthermore, geophysical studies for demonstrating paths of flow and for calculating the depth and volume of residual lakes from open-pit mining are discussed. (MSK) [Deutsch] Die Beitraege zu dem Workshop `Untersuchungen zu Flutungen in Tagebaurestseen` befassen sich unter anderem mit der hydrochemischen Entwicklung von Flutungsgewaessern, mit der Qualitaetsstabilitaet von Restseewaessern - insbesonders bei Einwirkung von Altlasten - und mit den Einfluessen von Solezufluessen bei Restseeflutung sowie mit Untersuchungen zur mikrobiologischen Oekologie in Bergbaurestseen. Desweiteren werden geophysikalische Untersuchungen zum Nachweis von Fliesswegen und die Tiefen- und Volumenberechnung eines Tagebaurestlochsees erlaeutert. (MSK)

  20. Contributions to the workshop `Residual ponds from open pit brown coal mining`; Beitraege zum Workshop ``Braunkohlebergbaurestseen``

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-31

    The contributions to the workshop ``Investigations on flooding in residual ponds from open-pit mining`` deal, inter alia, with the hydrochemical development of flushing waters, the stability of the water quality of residual lakes, especially under the impact of land contamination, the influence of brine receipts by flushed residual lakes, and with the microbiological ecology of residual lakes from mining. Furthermore, geophysical studies for demonstrating paths of flow and for calculating the depth and volume of residual lakes from open-pit mining are discussed. (MSK) [Deutsch] Die Beitraege zu dem Workshop `Untersuchungen zu Flutungen in Tagebaurestseen` befassen sich unter anderem mit der hydrochemischen Entwicklung von Flutungsgewaessern, mit der Qualitaetsstabilitaet von Restseewaessern - insbesonders bei Einwirkung von Altlasten - und mit den Einfluessen von Solezufluessen bei Restseeflutung sowie mit Untersuchungen zur mikrobiologischen Oekologie in Bergbaurestseen. Desweiteren werden geophysikalische Untersuchungen zum Nachweis von Fliesswegen und die Tiefen- und Volumenberechnung eines Tagebaurestlochsees erlaeutert. (MSK)

  1. Schule forscht Ansätze und Methoden zum forschenden Lernen

    CERN Document Server

    2009-01-01

    Kinderunis, Science Center, Experimentierlabore – der Markt der außerschulischen Wissenschaftsvermittlung für Kinder und Jugendliche boomt. Doch in den Schulen findet forschendes Lernen noch wenig Beachtung. Dabei fördert die Methode wichtige Fähigkeiten der Schüler und bietet Chancen für eine zukunftsweisende Schulentwicklung. Die Jugendlichen erarbeiten in Forschungsprojekten eigenständig wissenschaftliche Fragestellungen, suchen Lösungswege, dokumentieren und präsentieren ihre Erkenntisse: Sie trainieren so wissenschaftliches Arbeiten, Eigenverantwortlichkeit, Teamfähigkeit und Zeitmanagement. Lehrer werden zu Lernbegleitern, Schulen öffnen sich für Kooperationen und entwickeln Alternativen zum 45-minütigen Unterrichtstakt. Die Fachbeiträge von Andreas Müller, Ilka Parchmann, Gerhard Roth u. a. untermauern die Praxisberichte aus lern- und entwicklungspsychologischer Sicht. Die Autoren sind sich einig: Wer bereits in der Schule forscht, ist in Studium und Beruf einen großen Schritt voraus....

  2. Marianna Kaadi "Auk nr 8" linastub Põhja-Ameerikas

    Index Scriptorium Estoniae

    2011-01-01

    Marianna Kaadi dokumentaalfilmi "Auk nr 8" Põhja-Ameerika esilinastus toimub Full Frame filmifestivalil Durhamis, Põhja- Carolinas. Film on valitud ka Kanadas Torontos toimuva Hot Docs filmifestivali võistlusprogrammi

  3. Procedure guideline for radioiodine test. Version; Verfahrensanweisung zum Radioiodtest. Version 2

    Energy Technology Data Exchange (ETDEWEB)

    Dietlein, M.; Dressler, J.; Leisner, B.; Reiners, C.; Schicha, H. [Deutsche Gesellschaft fuer Nuklearmedizin (DGN) (Germany); Eschner, W.; Lassmann, M. [Deutsche Gesellschaft fuer Nuklearmedizin (DGN) (Germany); Deutsche Gesellschaft fuer Medizinische Physik (DGMP) (Germany)

    2003-06-01

    The version 2 of the procedure guideline for radioiodine test is an update of the guideline published in 1999. The following statements were added or modified: The procedure guideline discusses the pros and cons of a single measurement or of repeated measurements of the iodine-131 uptake and their optimal timing. Different formulas are described when one, two or three values of the radioiodine kinetic are available. The probe with a sodium iodide crystal, alternative or additionally the gamma-camera using the ROI-technique are instrumentations for the measurement of iodine-131 uptake. A possible source of error is an inappropriate measurement (sonography) of the target volume. The patients' preparation includes the withdrawal of antithyroid drugs 2-3 days before radioiodine administration. The patient has to avoid iodine-containing medication and the possibility of additives of iodide in vitamin- or electrolyte-supplementation has to be considered. (orig.) [German] Mit der Version 2 der Verfahrensanweisung zum Radioiodtest wird die in 1999 publizierte Verfahrensanweisung aktualisiert. Ergaenzungen und Modifikationen betreffen die notwendige Anzahl und die Zeitintervalle der Iod-131-Uptake-Messungen. Diskutiert werden die Argumente fuer die ein- oder mehrmalige Uptake-Messung. Berechnungsformeln fuer ein, zwei und drei Messpunkte werden angegeben. Als Messgeraete koennen die Uptake-Messsonde, alternativ oder zusaetzlich die Gammakamera mit ROI-Technik zum Einsatz kommen. Fehler bei der Volumetrie (Sonographie) des Zielvolumens gehen unmittelbar in die Berechnung der Therapieaktivitaet ein. Zur Vorbereitung der Patienten wird ein Absetzen der thyreostatischen Medikation 2 bis 3 Tage vor dem Radioiodtest empfohlen. Moeglich Iod(id) zusaetze in Multivitamin- und Spurenelementkombinationen sollten bedacht werden. (orig.)

  4. Pressure vessels dossier restoration according to NR-13 requirements; Enquadramento de vasos de pressao a norma NR-13

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Jose L. [PETROBRAS Transporte S.A. (TRANSPETRO), Rio de Janeiro, RJ (Brazil); Goncalves, Osorio C. [PETROBRAS Transporte S.A. (TRANSPETRO), Rio de Janeiro, RJ (Brazil)

    2005-07-01

    Pressure vessels are static pressurized equipment typical in oil industry facilities. In TRANSPETRO terminals and stations as well as in the whole PETROBRAS, these equipment can be found in the form of condenser accumulators, separators, heat exchangers, storage spheres and others. Because they work sustaining pressure and, many times flammable fluids, pressure vessels have a reasonable potential for hazard. For this reason, the NR-13 regulation was created. It deals with the safety in maintenance, operation and inspection of pressure vessels and boilers. During the compliance to the NR- 13 rules, a problem usually found is the lack of documents for different reasons. In this case, the NR-13 obligates the owner to recreate the vessel documentation under the responsibility of a chartered professional. This paper presents a case study where NR-13 rules were conformed by tasks involving documentation reconstruction based on information collected by means of inspection and tests performed on the field. (author)

  5. Literature study: State of knowledge on pollutant decomposition and transport in natural soils; Literaturstudie zum Thema: Darstellung und Bewertung des Wissensstandes zum Schadstoffabbau und -transport in natuerlichen Boeden

    Energy Technology Data Exchange (ETDEWEB)

    Koch, R. (ed.); Hahn, M.; Koennemann, T.; Mangold, S.; Ouerfelli, I.; Preuss, V.; Schoepke, R.; Sonntag, B.

    2000-07-01

    The use of natural soils for low-cost removal of precipitated water and slightly polluted waste water has become an issue in legal procedures concerning water management. Although groundwater protection is given high priority, authorities do not have sufficient data on long-term effects and decomposition effects during passage to the soil. The Hydrology Department of Brandenburgische Technische Universitaet Cottbus, on behalf of the Thuringian Environmental Authority, investigated the state of the art of discharge of precipitated water into natural soils. The investigation is the first in a series of which the following effects are expected: Faster results by preliminary assessment of the applicability of the technique envisaged, use of this low-cost technology for waste water treatment in consideration of surface water protection. [German] Die Nutzung natuerlicher Boeden zur kostenguenstigen Beseitigung von Niederschlagswasser aber auch von schwachbelastetem Abwasser ist eine Variante der Abwasserbeseitigung, die zunehmend Antragsgegenstand in wasserrechtlichen Verfahren ist. Der Schutz des Grundwassers ist dabei in besonderem Masse zu beachten. Die Einschaetzung der Eignung dieser Variante ist Behoerden aufgrund unzureichender Aussagen zum Langzeitverhalten und zu Abbaueffekten waehrend der Bodenpassage derzeit nicht moeglich. Durch die THUeRINGER LANDESANSTALT FUeR UMWELT erhielt der Lehrstuhl Wassertechnik der Brandenburgischen Technischen Universitaet Cottbus den Auftrag, im Rahmen einer Literaturstudie, den gegenwaertigen Stand der schadlosen Versickerung von Niederschlagswasser in natuerlichen Boeden aufzuzeigen. Die Literaturrecherche bildet den ersten Schritt einer Untersuchungsreihe, von der folgende Wirkungen erwartet werden: Verfahrensbeschleunigung durch Bewertbarkeit der Eignung der Behandlungsvariante, Nutzung dieser kostenguenstigen Umwelttechnologie zur Behandlung von Abwasser unter Beachtung des Gewaesserschutzes. (orig.)

  6. Expression of NR1 subunit of NMDA receptor and PSD-93/95 in rat hippocampus affected by NR1/NR2 antisense oligodeoxynucleotide

    Czech Academy of Sciences Publication Activity Database

    Vrajová, M.; Bubeníková-Valešová, V.; Klaschka, Jan

    2010-01-01

    Roč. 9, Suppl.1 (2010), S147 ISSN 1744-859X. [International Congress on Neurobiology and Clinical Psychopharmacology and European Psychiatric Association Conference on Treatment Guidance /1./. 19.11.2009-22.11.2009, Thessaloniki] R&D Projects: GA ČR GD309/09/H072; GA MŠk(CZ) 1M0517 Institutional research plan: CEZ:AV0Z10300504 Keywords : schizophrenia * animal model * NMDA receptor * aODN-NR1/NR2 * PPI Subject RIV: FL - Psychiatry, Sexuology

  7. Die Darstellung Rudolf Virchows in der Vossischen Zeitung im Zeitraum vom 1. Januar 1844 bis zum 31. Dezember 1865

    OpenAIRE

    Feddersen, Lars Harald

    2010-01-01

    DIE DARSTELLUNG RUDOLF VIRCHOWS IN DER VOSSISCHEN ZEITUNG IM ZEITRAUM VOM 1. JANUAR 1844 BIS ZUM 31. DEZEMBER 1865 Rudolf Virchow erforschte u.a. als Pathologe, Hygieniker, Anthropologe, Ethnologe und Prähistoriker bedeutende Grundlagen der modernen Wissenschaft. Diese vielschichtigen Tätigkeitsbereiche zeichnen ihn als einen der bedeutendsten Wissenschaftler v.a. der Medizingeschichte aus. Als Politiker engagierte sich Virchow in erster Linie im sozial- und gesellschaftspolitischen Bereich. ...

  8. Nuclear receptor 4A (NR4A) family - orphans no more.

    Science.gov (United States)

    Safe, Stephen; Jin, Un-Ho; Morpurgo, Benjamin; Abudayyeh, Ala; Singh, Mandip; Tjalkens, Ronald B

    2016-03-01

    The orphan nuclear receptors NR4A1, NR4A2 and NR4A3 are immediate early genes induced by multiple stressors, and the NR4A receptors play an important role in maintaining cellular homeostasis and disease. There is increasing evidence for the role of these receptors in metabolic, cardiovascular and neurological functions and also in inflammation and inflammatory diseases and in immune functions and cancer. Despite the similarities of NR4A1, NR4A2 and NR4A3 and their interactions with common cis-genomic elements, they exhibit unique activities and cell-/tissue-specific functions. Although endogenous ligands for NR4A receptors have not been identified, there is increasing evidence that structurally-diverse synthetic molecules can directly interact with the ligand binding domain of NR4A1 and act as agonists or antagonists, and ligands for NR4A2 and NR4A3 have also been identified. Since NR4A receptors are key factors in multiple diseases, there are opportunities for the future development of NR4A ligands for clinical applications in treating multiple health problems including metabolic, neurologic and cardiovascular diseases, other inflammatory conditions, and cancer. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Success in the protection of the ozone layer is at risk. An interview concerning the German view on CFC- and H-CFC-phaseout all over the world; Bisherige Erfolge zum Schutz der Ozonschicht sind gefaehrdet. Ein Fachgespraech ueber die deutsche Meinung zum FCKW- und H-FCKW-Phaseout ueberall in der Welt

    Energy Technology Data Exchange (ETDEWEB)

    Kraus, H.W.; Weissenborn, P.

    1995-11-01

    This article repeats an interview with the commissioner of international measures for the protection of the ozone layer of the Federal Ministry of the Environment which took place in the run-up to the 7th conference of the signatory states of the Montreal Protocol. (BWI) [Deutsch] Der vorliegende Beitrag gibt ein Interview wieder, das mit dem Beauftragten fuer internationale Massnahmen zum Schutz der Ozonschicht beim Bundesumweltministerium im Vorfeld der 7. Konferenz der Vertragsstaaten zum Montrealer Protokoll gefuehrt wurde. (BWI)

  10. Modulation of NMDA Receptor Properties and Synaptic Transmission by the NR3A Subunit in Mouse Hippocampal and Cerebrocortical Neurons

    Science.gov (United States)

    Tong, Gary; Takahashi, Hiroto; Tu, Shichun; Shin, Yeonsook; Talantova, Maria; Zago, Wagner; Xia, Peng; Nie, Zhiguo; Goetz, Thomas; Zhang, Dongxian; Lipton, Stuart A.; Nakanishi, Nobuki

    2015-01-01

    Expression of the NR3A subunit with NR1/NR2 in Xenopus oocytes or mammalian cell lines leads to a reduction in N-methyl-D-aspartate (NMDA)-induced currents and decreased Mg2+ sensitivity and Ca2+ permeability compared with NR1/NR2 receptors. Consistent with these findings, neurons from NR3A knockout (KO) mice exhibit enhanced NMDA-induced currents. Recombinant NR3A can also form excitatory glycine receptors with NR1 in the absence of NR2. However, the effects of NR3A on channel properties in neurons and synaptic transmission have not been fully elucidated. To study physiological roles of NR3A subunits, we generated NR3A transgenic (Tg) mice. Cultured NR3A Tg neurons exhibited two populations of NMDA receptor (NMDAR) channels, reduced Mg2+ sensitivity, and decreased Ca2+ permeability in response to NMDA/glycine, but glycine alone did not elicit excitatory currents. In addition, NMDAR-mediated excitatory postsynaptic currents (EPSCs) in NR3A Tg hippocampal slices showed reduced Mg2+ sensitivity, consistent with the notion that NR3A subunits incorporated into synaptic NMDARs. To study the function of endogenous NR3A subunits, we compared NMDAR-mediated EPSCs in NR3A KO and WT control mice. In NR3A KO mice, the ratio of the amplitudes of the NMDAR-mediated component to α-amino-3-hydroxy-5-methyl-4-isox-azolepropionic acid receptor-mediated component of the EPSC was significantly larger than that seen in WT littermates. This result suggests that NR3A subunits contributed to the NMDAR-mediated component of the EPSC in WT mice. Taken together, these results show that NR3A subunits contribute to NMDAR responses from both synaptic and extra-synaptic receptors, likely composed of NR1, NR2, and NR3 subunits. PMID:18003876

  11. Differential functions of NR2A and NR2B in short-term and long-term memory in rats.

    Science.gov (United States)

    Jung, Ye-Ha; Suh, Yoo-Hun

    2010-08-23

    N-methyl-D-aspartate receptors (NMDARs) are glutamate receptors implicated in synaptic plasticity and memory function. The specific functions of NMDA receptor subunits NR2A and NR2B have not yet been fully determined in the different types of memory. Nine Wistar rats (8-weeks-old) were subjected to the Morris water maze task to evaluate the memory behaviorally. Quantitative analysis of NR1, NR2A, and NR2B levels in the right and left forebrain of rats was performed and subunit associations with different types of memory were investigated using the Morris water maze task. Right forebrain NR2A expression was significantly increased and correlated with faster escape time onto a hidden platform, indicating involvement of short-term memory, because of the training time interval. Right forebrain NR2B expression was positively associated with long-term memory lasting 24-h (h). In the left forebrain, NR2B expression was positively related to 72-h long-term memory. In conclusion, the functions of NR2A and NR2B receptors were differentially specialized in short-term and long-term memory, depending on the right or left forebrain.

  12. Analysis of NR3A receptor subunits in human native NMDA receptors

    DEFF Research Database (Denmark)

    Nilsson, Anna; Eriksson, Maria; Muly, E Chris

    2007-01-01

    NR3A, representing the third class of NMDA receptor subunits, was first studied in rats, demonstrating ubiquitous expression in the developing central nervous system (CNS), but in the adult mainly expressed in spinal cord and some forebrain nuclei. Subsequent studies showed that rodent and non-human...... primate NR3A expression differs. We have studied the distribution of NR3A in the human CNS and show a widespread distribution of NR3A protein in adult human brain. NR3A mRNA and protein were found in all regions of the cerebral cortex, and also in the subcortical forebrain, midbrain and hindbrain. Only...... very low levels of NR3A mRNA and protein could be detected in homogenized adult human spinal cord, and in situ hybridization showed that expression was limited to ventral motoneurons. We found that NR3A is associated with NR1, NR2A and NR2B in adult human CNS, suggesting the existence of native NR1-NR2...

  13. Von der Pionierarbeit zum Nachschlagewerk From Pioneering Work to Reference Work

    Directory of Open Access Journals (Sweden)

    Sylvia Mieszkowski

    2006-11-01

    Full Text Available 1995 erschien der von Hadumod Bußmann und Renate Hof herausgegebene Sammelband Genus. Zur Geschlechterdifferenz in den Kulturwissenschaften. Zum 10jährigen Jubiläum haben sich die beiden Herausgeberinnen noch einmal mit dem Kröner Verlag zusammengetan, haben teils dieselben, teils neue Autorinnen um sich geschart und Genus einen neuen Auftritt verschafft. Der aktuelle Untertitel benennt die Erweiterung, die statt einer bloßen Aktualisierung des ersten Bandes vorgenommen wurde: Geschlechterforschung/Gender Studies in den Kultur- und Sozialwissenschaften. Ein Handbuch.The collected volume Genus. On Gender Difference in Cultural Studies (Genus. Zur Geschlechterdifferenz in den Kulturwissenschaften, edited by Hadumod Bußmann and Renate Hof, was published in 1995. In honor of ten years of publication both editors came together with the publishing house Kröner Verlag once again, bringing with them some of the same as well as some new authors, in order to provide Genus a renewed appearance on the academic stage. Instead of merely revising the original volume, the editors planned its expansion. The subtitle points to the result: Gender Studies in Culture and Social Studies. A Handbook (Geschlechterforschung/Gender Studies in den Kultur- und Sozialwissenschaften. Ein Handbuch.

  14. Ein statistisches Modell zum Einfluß der thermischen Bewegung auf NMR-Festkörperspektren

    Science.gov (United States)

    Ploss, W.; Freude, D.; Pfeifer, H.; Schmiedel, H.

    Es wird ein statistisches Modell zum Einfluß der thermischen Bewegung auf die NMR-Linienform vorgestellt, das die Verschmälerung von Festkörper-Spektren bei wachsender Temperatur beschreibt. Das Modell geht von der Annahme aus, daß nach einer Ortsveränderung eines Kerns infolge thermischer Bewegung jede beliebige Kernresonanzfrequenz mit der durch das Festkörperspektrum vorgegebenen Wahrscheinlichkeit angenommen werden kann. Am Beispiel der Festkörper-Gaußlinie wird der Unterschied zu dem bekannten Modell von ANDERSON und WEISS verdeutlicht.Translated AbstractA Statistical Model for the Influence of Thermal Motion on N. M. R. Spectra in SolidsA theory is proposed which allows to describe the narrowing of n. m. r.-line width in the presence of thermal motions of the spins. The model is based on the assumption, that the local resonance frequency of a given spin immediately after the jump is distributed according to the n. m. r.-line shape of the rigid lattice. The difference to the well-known ANDERSON-WEISS-model of spectral narrowing is demonstrated for a gaussian line shape.

  15. Akupunktur bei Sterilität und zum Schwangerschaftserhalt: Eine Übersicht mit Forschungsidee

    Directory of Open Access Journals (Sweden)

    Ots T

    2011-01-01

    Full Text Available Die Unterstützung der weiblichen Fertilität und der Schwangerschaft mittels Akupunktur hat während der vergangenen Dekade viele Anhänger gefunden. Dieser Artikel gibt eine Übersicht über die hierzu veröffentlichten wichtigsten Studien. Die Wirkung der Akupunktur wird als effektiv und effizient beschrieben. In einem zweiten Ansatz stellt der Autor eine vereinfachte Form der Akupunktur auf dem Boden der Segment-Anatomie vor. Dieser kuti-viszeralen, segmentalen Reizung der gynäkologischen Organe könnte innerhalb der gynäkologischen Praxis große Bedeutung zukommen, da sie leicht erlernbar (innerhalb eines Tages und einfach durchführbar ist. Die Zielvorstellung ist ein integratives Vorgehen in der Hand der Gynäkologen. Vorbereitend hierzu ist eine multizentrische randomisierte kontrollierte Studie notwendig, die diese beiden Ansätze innerhalb der Akupunktur als Komplement zum gynäkologischen Prozedere der IVF untersucht.

  16. PAH analysis in Leipzig allotment soils; Untersuchungen zum Gefaehrdungspotential polycyclischer aromatischer Kohlenwasserstoffe (PAK) in Leipziger Kleingartenboeden

    Energy Technology Data Exchange (ETDEWEB)

    Bittrich, R.; Butze, B.; Mueller, S.; Prawalsky, R.; Stoye, H. [Umwelt-Consult e.V., Leipzig (Germany)

    2000-09-01

    Soils in 29 allotments were analyzed systematically with a view to the following aspects: Concentration ratios of the 16 components analyzed. Occurrence and classification of so-called PAH patterns. Interdependences between PAH patterns and soil features. PAH concentrations and soil-immanent buffer characteristics (humus concentration, pH, clay concentration, sesquioxide concentrations, exchange capacity). [German] Die vorliegende Arbeit konzentriert sich auf die Untersuchung der PAK-Belastung kleingaertnerisch genutzter Boeden. Die hier vorgestellten Ergebnisse resultieren aus Probjekten von Umwelt-Consult e.V. aus den Jahren 1995 bis 1997 im Auftrag der Stadt Leipzig und dem unter fachlicher Begleitung des Referates Geochemie der Abt. Boden/Geochemie vom LfUG gefoerderten Forschungsvorhaben 'Untersuchungen zum Gefaehrdungspotential polycyclischer aromatischer Kohlenwasserstoffe (PAK) in Boeden der Stadt Leipzig'. Hierbei wurden systematisch Boeden in 29 Kleingartenanlagen untersucht. Folgende Fragestellungen sollten beantwortet werden: Stehen die PAK-Konzentrationen der 16 analysierten Einzelkomponenten in bestimmten Groessenverhaeltnissen zueinander? Sind sogenannte PAK-Muster zu erkennen und lassen sich diese klassifizieren? Welche Beziehungen gibt es zwischen PAK-Mustern und Bodenmerkmalen? Korrespondieren die PAK-Konzentrationen (Gesamt-PAK, Einzelkomponenten) im Boden und deren bodenhorizont-bezogene Abfolge mit der Auspraegung bodenimmanenter Puffermerkmale (Humusgehalt, pH-Wert, Tongehalt, Gehalt an Sesquioxiden, Austauschkapazitaet)? (orig.)

  17. LENRA as compatibilizer in NR/HDPE blends

    International Nuclear Information System (INIS)

    Mahathir Mohamed; Dahlan Mohd

    2004-01-01

    Polymer blends of 60/40 NR/HDPE were prepared using Brabender PL2000 plasticorder with 60g capacity. The blends were added with radiation sensitive natural rubber (NR)-based compatibilizer, known as LENRA. They were irradiated in air with electron beam radiation at various doses. The efficacy of the compatibilizer was monitored by measuring various properties of the blends such as physical and dynamic mechanical properties including morphological studies by electron microscopic technique. Early results show that the addition of LENRA improves the properties of the TPNR blends. (Author)

  18. LENRA as compatibilizer in NR/HDPE blends

    International Nuclear Information System (INIS)

    Dahlan Mohd; Mahathir Mohamed

    2006-01-01

    Polymer blends of 60/40 NR/RDPE were prepared using Brabender PL2000 Plasticorder with 60 g capacity. The blends were added with radiation-sensitive natural rubber (NR)-based compatibilizer, known as LENRA. They were irradiated with electron-beam radiation at various doses. The efficacy of the compatibilizer was monitored by measuring various properties of the blends such as physical and dynamic mechanical properties including morphological studies by electron microscopic technique. Early results show that the addition of LENRA improves the properties of the TPNR blends. (Author)

  19. Practical experience with biodegradable biomass waste bags in several different German composting plants; Praxiserfahrungen zum Abbau kompostierbarer Bioabfallsaecke auf verschiedenen Kompostierungsanlagen in Deutschland

    Energy Technology Data Exchange (ETDEWEB)

    Ziermann, Andreas; Schmidt, Bettina [C.A.R.M.E.N. e.V., Straubing (Germany)

    2012-11-01

    The study intended to find out how fast biodegradable biomass waste bags are degraded in practical conditions in composting and fermentation plants. The plants differ with regard to the processes employed; further, rotting times may be much shorter in practice than the twelve weeks requested by DIN EN 13432 and DIN EN 14995. For the study, plant types were selected that are practically relevant for biomass waste utilisation in Germany. (orig.) [German] Ziel der vorliegenden Studie war es, herauszufinden, wie schnell kompostierbare Bioabfallsaecke unter Praxisbedingungen in verschiedenen Kompost- und Vergaerungsanlagentypen abgebaut werden. Zum einen bestehen teilweise grosse verfahrenstechnische Unterschiede zwischen den Anlagentypen, zum anderen sind die Rottezeiten in der Praxis zum Teil wesentlich kuerzer, als die in der DIN EN 13432 und DIN EN 14995 geforderten zwoelf Wochen. Fuer die Studie wurden Anlagentypen ausgewaehlt, die fuer die Verwertung von Bioabfaellen in Deutschland praxisrelevant sind. (orig.)

  20. Co-Application of Corticosterone and Growth Hormone Upregulates NR2B Protein and Increases the NR2B:NR2A Ratio and Synaptic Transmission in the Hippocampus

    Directory of Open Access Journals (Sweden)

    Ghada S. Mahmoud

    2014-10-01

    Full Text Available Objectives: This in vitro study aimed to investigate the possible mechanism underlying the protective effect of growth hormone (GH on hippocampal function during periods of heightened glucocorticoid exposure. Methods: This study was conducted between January and June 2005 at the Joan C. Edwards School of Medicine, Marshall University, in Huntington, West Virginia, USA. The effects of the co-application of GH and corticosterone (CORT were tested at different concentrations on the field excitatory postsynaptic potentials (fEPSPs of the hippocampal slices of rats in two different age groups. Changes in the protein expression of N-methyl-D-aspartate receptor (NMDAR subunits NR1, NR2B and NR2A were measured in hippocampal brain slices treated with either artificial cerebrospinal fluid (ACSF, low doses of CORT alone or both CORT and GH for three hours. Results: The co-application of CORT and GH was found to have an additive effect on hippocampal synaptic transmission compared to either drug alone. Furthermore, the combined use of low concentrations of GH and CORT was found to have significantly higher effects on the enhancement of fEPSPs in older rats compared to young ones. Both GH and CORT enhanced the protein expression of the NR2A subunit. Simultaneous exposure to low concentrations of GH and CORT significantly enhanced NR2B expression and increased the NR2B:NR2A ratio. In contrast, perfusion with CORT alone caused significant suppression in the NR1 and NR2B protein expression and a decrease in the NR2B:NR2A ratio. Conclusion: These results suggest that NMDARs provide a potential target for mediating the GH potential protective effect against stress and age-related memory and cognitive impairment.

  1. Genetische Veränderungen an primären Prostatakarzinom-Biopsien in Korrelation zum klinischen Verlauf

    Directory of Open Access Journals (Sweden)

    Steiner T

    2002-01-01

    Full Text Available Die der Entstehung des Prostatakarzinoms zugrundeliegenden bzw. mit einem Progreß vergesellschafteten genetischen Veränderungen sind noch weitgehend unbekannt. Ziel der vorliegenden retrospektiven Untersuchung war die Erfassung genetischer Aberrationen an paraffineigebettetem Material primärer Prostatakarzinom-Biopsien und die Korrelation der Daten zum klinischen Verlauf. In 28 karzinomtragenden primären Prostatabiopsien wurden die Tumorareale gekennzeichnet. Nach Dissektion der Tumorzellen wurden die Proben mittels "comparative genomic hybridization" (CGH analysiert. Daten zum klinischen Verlauf der Patienten wurden erfaßt. Die mittlere Nachbeobachtungszeit betrug 36 Monate, alle Patienten wurden hormonablativ behandelt. 11 Patienten (39 % wiesen unter laufender Therapie einen Tumorprogreß auf. In 25 (88 % der 28 untersuchten Tumorareale konnten genetische Aberrationen detektiert werden, im Mittel fanden wir 4,8 Aberrationen je Tumor. Das Verhältnis Verlust / Zugewinn betrug 1,3 / 1. Folgende chromosomale Veränderungen wurden häufig festgestellt: Verluste von 6q in 40 %; von 8p in 32 %; von 16q in 21 %; von 18q in 21 %; Zugewinne von 8q in 32 %; von 17 in 43 %. In Korrelation zum klinischen Verlauf fand sich ein Zugewinn von 8q signifikant häufiger bei Patienten, welche im Beobachtungszeitraum einen Tumorprogreß entwickelten (64 % vs. 12 %; p = 0,0001. In der primären Tumorbiopsie waren bei progredienten Patienten im Mittel 5,3, bei Patienten ohne Tumorprogreß 3,8 Aberrationen je Tumor nachzuweisen. Unsere Ergebnisse zeigen, daß Veränderungen der Chromosomen 6q, 8, 16q, 17 und 18q regelmäßig beim Prostatakarzinom auftreten. Karzinome mit klinisch ungünstigem Verlauf weisen bereits in der primären Biopsie eine hohe genetische Instabilität auf.

  2. Molecular mechanism of ligand recognition by NR3 subtype glutamate receptors

    Energy Technology Data Exchange (ETDEWEB)

    Yao, Yongneng; Harrison, Chris B.; Freddolino, Peter L.; Schulten, Klaus; Mayer, Mark L. (UIUC); (NIH)

    2008-10-27

    NR3 subtype glutamate receptors have a unique developmental expression profile, but are the least well-characterized members of the NMDA receptor gene family, which have key roles in synaptic plasticity and brain development. Using ligand binding assays, crystallographic analysis, and all atom MD simulations, we investigate mechanisms underlying the binding by NR3A and NR3B of glycine and D-serine, which are candidate neurotransmitters for NMDA receptors containing NR3 subunits. The ligand binding domains of both NR3 subunits adopt a similar extent of domain closure as found in the corresponding NR1 complexes, but have a unique loop 1 structure distinct from that in all other glutamate receptor ion channels. Within their ligand binding pockets, NR3A and NR3B have strikingly different hydrogen bonding networks and solvent structures from those found in NR1, and fail to undergo a conformational rearrangement observed in NR1 upon binding the partial agonist ACPC. MD simulations revealed numerous interdomain contacts, which stabilize the agonist-bound closed-cleft conformation, and a novel twisting motion for the loop 1 helix that is unique in NR3 subunits.

  3. Fast biodegradation of toxic bisphenol a by Pseudomonas aeruginosa NR.22 (Ps.NR.22) isolated from Malaysian local lake

    Science.gov (United States)

    Him, Nik Raikhan Nik; Zainuddin, Mohammad Fiqri; Basha, Anuar Zain Anuar

    2017-12-01

    The paper focused on microbial degradation of Bisphenol A (BPA) as a safe and fast method to reduce BPA contamination in water. BPA is found in waste water, sea water and home water pipeline and it is nondegradable pollutant. Biodegradation is suggested to be practical solution for large volume of BPA. Biodegradation plays an important role and the effect of low concentration significantly decreased the degradation rate. Pseudomonas aeruginosa NR.22 (Ps.NR.22) which has been isolated from a lake at Seksyen 2, Shah Alam, was used. In Malaysia, Ps.NR.22 isolation agar is used for the BPA degradation process. It was stained with Gram negative-rod shaped bacteria that confirmed to carry a 16S rRNA gene. BPA as a sole carbon has been tested at various concentrations. The research showed that BPA was degraded at 10, 20, 30, 40 and 50 ppm and the bacteria growth rate was excellent in 20 ppm BPA. Degradation of BPA was carried out for 9 hours to 18 hours and the maximum degradation was recorded at 9 hours. The value of the highest peak of growth at 540 nm was 2.0617 using 20 ppm BPA. This novel Pseudomonas aeruginosa NR.22 has great potential to be used in waste water treatment.

  4. Firewall systems for protection of data communication in power stations; Firewall-Systeme zum Schutz der Datenkommunikation in Kraftwerken

    Energy Technology Data Exchange (ETDEWEB)

    Schoenfeld, P. [Siemens AG KWU (Germany)

    1998-12-31

    Networking of power plant computer systems is becoming increasingly common. Furthermore, instrumentation and control systems are being connected with general power plant networks to make process information available to administrative personnel. This increases the risk of accidental or intentional access from outside the system (e.g. by hackers). Firewall systems are used to prevent such outside access. A firewall is the sole gateway between two networks which are to be kept separate, and is used to filter and control access to the protected network. Cryptography and personal chip cards are also used to provide additional protection. (orig.) [Deutsch] Rechnersysteme im Kraftwerk werden heute mehr und mehr vernetzt. Auch werden Rechnersysteme zum Messen, Steuern und Regeln (sogenannte Instrumentation and Control Systems) mit allgemeinen Kraftwerksnetzen verbunden, um Prozessinformationen auch in der Verwaltung zugaenglich zu machen. Damit waechst die Gefahr von zufaelligen oder beabsichtigten Eingriffen von aussen (z.B. Hacker). Zum Schutz vor solchen Eingriffen von aussen werden sogenannte Firewall-Systeme eingesetzt. Ein Firewall ist der alleinige Uebergang zwischen zwei zu trennenden Netzen und dient zur Filterung und Kontrolle der Zugriffe auf ein zu schuetzendes Netz. Ergaenzende Schutzmassnahmen sind der Einsatz kryptographischer Verfahren, sowie persoenlicher Chipkarten. (orig.)

  5. Firewall systems for protection of data communication in power stations; Firewall-Systeme zum Schutz der Datenkommunikation in Kraftwerken

    Energy Technology Data Exchange (ETDEWEB)

    Schoenfeld, P [Siemens AG KWU (Germany)

    1999-12-31

    Networking of power plant computer systems is becoming increasingly common. Furthermore, instrumentation and control systems are being connected with general power plant networks to make process information available to administrative personnel. This increases the risk of accidental or intentional access from outside the system (e.g. by hackers). Firewall systems are used to prevent such outside access. A firewall is the sole gateway between two networks which are to be kept separate, and is used to filter and control access to the protected network. Cryptography and personal chip cards are also used to provide additional protection. (orig.) [Deutsch] Rechnersysteme im Kraftwerk werden heute mehr und mehr vernetzt. Auch werden Rechnersysteme zum Messen, Steuern und Regeln (sogenannte Instrumentation and Control Systems) mit allgemeinen Kraftwerksnetzen verbunden, um Prozessinformationen auch in der Verwaltung zugaenglich zu machen. Damit waechst die Gefahr von zufaelligen oder beabsichtigten Eingriffen von aussen (z.B. Hacker). Zum Schutz vor solchen Eingriffen von aussen werden sogenannte Firewall-Systeme eingesetzt. Ein Firewall ist der alleinige Uebergang zwischen zwei zu trennenden Netzen und dient zur Filterung und Kontrolle der Zugriffe auf ein zu schuetzendes Netz. Ergaenzende Schutzmassnahmen sind der Einsatz kryptographischer Verfahren, sowie persoenlicher Chipkarten. (orig.)

  6. Kino filmų titrai kaip kalbų mokymosi priemonė. Untertitel als Mittel zum Fremdsprachenerwerb

    Directory of Open Access Journals (Sweden)

    Alina Baravykaitė

    2007-01-01

    Full Text Available Das in den letzten Jahren spürbare Bestreben der litauischen Gesetzgeber, Untertitelung von Fernsehfilmen zwecks Fremdsprachenerwerb gesetzlich anzuordnen, löste eine Auseinandersetzung in der Öffentlichkeit aus, die sich in der Presse sowie im Internet widerspiegelt und als Anstoß zum vorliegenden Beitrag dient. Diese Arbeit stellt den Versuch dar, die Möglichkeiten und Grenzen der Untertitelung als eines Mittels zum Fremdsprachenerwerb zu untersuchen. Auf Grund der fehlenden wissenschaftlichen Forschungen in diesem Bereich sollen dabei empirische Betrachtungen und die vergleichende Textanalyse von Original und Übersetzung als Hilfe dienen. Auch wenn die Relevanz der Untertitelung für den Fremdsprachenerwerb nicht bestreitbar ist, ist sie aber zugleich nicht zu überschätzen: Durch die lediglich für diese Form der audiovisuellen Übersetzung charakteristischen Merkmale (kurze Einblendezeit, kondensierte Sprache und begrenzte Übertragungsmöglichkeiten von Realia-Begriffen, Phraseologismen, Intertextualismen, dem Wortspiel etc. bleiben dem Zuschauer oft sprachliche Stilmittel vorenthalten und der in allen Übersetzungsbereichen vorkommende unausweichliche Sinn- und Wirkungsverlust des Originals wird erheblich verstärkt. Anbetracht der erwähnten Aspekte und der häufigen fehlerhaften Übersetzungen sollen Untertitel nur als Hilfsmittel beim Fremdsprachenerwerb angewendet werden, wobei herkömmliche Lehr- und Lernstrategien von Fremdsprachen in den Vordergrund treten müssen.

  7. Evaluation von E-Learning: Checklisten, Kriterienkataloge oder Evaluationskonzepte? Zum Stand der Bewertungsverfahren für E-Learning-Arrangements

    Directory of Open Access Journals (Sweden)

    Ulf-Daniel Ehlers

    2017-09-01

    Full Text Available Qualität in Bildungsmaßnahmen - als Legitimation oder aus wissenschaftlichem Interesse - ist ein Thema von hoher Bedeutung, auch - und gerade - im E-Learning. E-Learning, als eine noch relativ junge, nicht völlig etablierte, aber - so stellt sich immer häufiger heraus – kostenintensive Bildungsform, muss sich immer wieder der kritischen Frage nach der Qualität stellen, zumeist assoziiert mit Lernerfolg. Vielfältige Instrumente, um die Qualität zu überprüfen oder zu entwickeln, begleitend oder im Nachhinein, sind bislang konzipiert worden (vgl. Ehlers/Pawlowski/Goertz 2003. Der Artikel gibt einen Überblick zum derzeitigen Stand der Dinge in Bezug auf zwei dieser Verfahrensweisen: zum einen geht es dabei um die Beurteilungen anhand von Kriterienkatalogen bzw. Checklisten und zum anderen um Evaluationsverfahren. Obwohl vielfach bereits umfassendere Managementsysteme zum Einsatz kommen, die die Qualität bei Anbietern kontinuierlich verbessern sollen, haben beide Verfahren eine steigende Bedeutung - da auch Managementverfahren immer wieder auf Kriterien oder Evaluationsverfahren zurückgreifen.

  8. Keim, Karl-Dieter, Das Fenster zum Raum : Traktat über die Erforschung sozialräumlicher Transformation / [rezensiert von] Helmut Klüter

    OpenAIRE

    Klüter, Helmut

    2009-01-01

    Rezensiertes Werk: Keim, Karl-Dieter: Das Fenster zum Raum : Traktat über die Erforschung sozialräumlicher Transformation / Karl-Dieter Keim. - Opladen : Leske + Budrich, 2003. - 171 S. : graph. Darst. - (Lehrtexte : Sozialwissenschaften) ISBN 3-8100-4050-9

  9. Roussel. Sinfonien Nr. 3 g-Moll op. 42 und Nr. 4 A-Dur op. 5, Neeme Järvi / Christoph Schlüren

    Index Scriptorium Estoniae

    Schlüren, Christoph

    1995-01-01

    Uuest heliplaadist "Roussel. Sinfonien Nr. 3 g-Moll op. 42 und Nr. 4 A-Dur op. 53, Bacchus et Ariane op. 43 (Zweite Orchestersuite). Sinfonietta für Streichorchester d-Moll op. 52. Detroit Symphony Orchester, N. Järvi". Chandos/Koch CD 7007(WD: 70'10") DDD

  10. Expression and Functional Pathway Analysis of Nuclear Receptor NR2F2 in Ovarian Cancer

    Science.gov (United States)

    Hawkins, Shannon M.; Loomans, Holli A.; Wan, Ying-Wooi; Ghosh-Choudhury, Triparna; Coffey, Donna; Xiao, Weimin; Liu, Zhandong; Sangi-Haghpeykar, Haleh

    2013-01-01

    Context: Recent evidence implicates the orphan nuclear receptor, nuclear receptor subfamily 2, group F, member 2 (NR2F2; chicken ovalbumin upstream promoter-transcription factor II) as both a master regulator of angiogenesis and an oncogene in prostate and other human cancers. Objective: The objective of the study was to determine whether NR2F2 plays a role in ovarian cancer and dissect its potential mechanisms of action. Design, Setting, and Patients: We examined NR2F2 expression in healthy ovary and ovarian cancers using quantitative PCR and immunohistochemistry. NR2F2 expression was targeted in established ovarian cancer cell lines to assess the impact of dysregulated NR2F2 expression in the epithelial compartment of ovarian cancers. Results: Our results indicate that NR2F2 is robustly expressed in the stroma of healthy ovary with little or no expression in epithelia lining the ovarian surface, clefts, or crypts. This pattern of NR2F2 expression was markedly disrupted in ovarian cancers, in which decreased levels of stromal expression and ectopic epithelial expression were frequently observed. Ovarian cancers with the most disrupted patterns of NR2F2 were associated with significantly shorter disease-free interval by Kaplan-Meier analysis. Targeting NR2F2 expression in established ovarian cancer cell lines enhanced apoptosis and increased proliferation. In addition, we found that NR2F2 regulates the expression of NEK2, RAI14, and multiple other genes involved in the cell cycle, suggesting potential pathways by which dysregulated expression of NR2F2 impacts ovarian cancer. Conclusions: These results uncover novel roles for NR2F2 in ovarian cancer and point to a unique scenario in which a single nuclear receptor plays potentially distinct roles in the stromal and epithelial compartments of the same tissue. PMID:23690307

  11. Characterisation of the human NMDA receptor subunit NR3A glycine binding site

    DEFF Research Database (Denmark)

    Nilsson, A; Duan, J; Mo-Boquist, L-L

    2007-01-01

    In this study, we characterise the binding site of the human N-methyl-d-aspartate (NMDA) receptor subunit NR3A. Saturation radioligand binding of the NMDA receptor agonists [(3)H]-glycine and [(3)H]-glutamate showed that only glycine binds to human NR3A (hNR3A) with high affinity (K(d)=535nM (277...

  12. Research on non-destructive testing (NDT) aerospace igniter fuse with neutron radiography (NR)

    International Nuclear Information System (INIS)

    Mo Dawei; Liu Yisi; Cai Qingsheng; Chen Boxian

    1995-01-01

    The research works, facilities and results of NDT aerospace igniter fuse with neutron radiography at Tsinghua University swimming-pool reactor are introduced. The image quality (NR) of ASTM E545-85 I level was approached. The NR experimental research of the typical and possible defects was performed. The theoretical analysis was performed too. The feasibility of NDT aerospace igniter fuse with NR was proved experimentally

  13. Blood compatibility assessment of graft copolymer (NR-g-DMAA) tubes

    International Nuclear Information System (INIS)

    Razzak, M.T.; Otsuhata, Kazushige; Tabata, Yoneho; Ohashi, Fumio; Takeuchi, Atsuki

    1992-01-01

    Graft copolymer (NR-g-DMAA) tubes have been prepared using simultaneous radiation induced grafting of N,N-dimethyl-acrylamide, (DMAA) onto natural rubber (NR) tubes. The blood compatibility of the NR-g-DMAA tubes was assessed with three methods, namely in vitro test, ex vivo once through test and ex vivo loops test. In the case of the in vitro test, a simple whole blood contacting procedure has been employed. The ex vivo once through test involves the exposing of NR-g-DMAA tubes with once through flow of fresh canine blood and then it was inspected for any evidence of clot. In the case of ex vivo loops test, the NR-g-DMAA tube was implanted at external jugular vein of a mongrel canine and the blood flow in the NR-g-DMAA tube was detected with an ultrasonic flow meter. It was found that the blood compatibility of NR-g-DMAA tubes is improved significantly with the increasing degree of grafting. All the NR-g-DMAA tubes having a degree of grafting of about 30 wt% or more exhibit good blood compatibility. It was found that the blood compatibility of the NR-g-DMAA tube is better than that of a medical grade silicon rubber (SiR) tube. (Author)

  14. Reduced levels of NR1 and NR2A with depression-like behavior in different brain regions in prenatally stressed juvenile offspring.

    Directory of Open Access Journals (Sweden)

    Hongli Sun

    Full Text Available Adolescence is a time of continued brain maturation, particularly in limbic and cortical regions, which undoubtedly plays a role in the physiological and emotional changes. Juvenile rats repeatedly exposed to prenatal stress (PS exhibit behavioral features often observed in neuropsychiatric disorders including depression. However, to date the underlying neurological mechanisms are still unclear. In the current study, juvenile offspring rats whose mothers were exposed to PS were evaluated for depression-related behaviors in open field and sucrose preference test. NMDA receptor subunits NR1 and NR2A in the hippocampus, frontal cortex and striatum were assayed by western blotting. The results indicated that PS resulted in several behavioral anomalies in the OFT and sucrose preference test. Moreover, reduced levels of NMDA receptor subunits NR1 and NR2A in the hippocampus, and NR1 in prefrontal cortex and striatum of prenatally stressed juvenile offspring were found. Treatment with MK-801 to pregnant dams could prevent all those changes in the juvenile offspring. Collectivity, these data support the argument that PS to pregnant dams could induce depression-like behavior, which may be involved with abnormal expression of NR1 and NR2A in specific brain regions, and MK-801 may have antidepressant-like effects on the juvenile offspring.

  15. The N-methyl-D-aspartate receptor subunits NR2A and NR2B bind to the SH2 domains of phospholipase C-gamma.

    Science.gov (United States)

    Gurd, J W; Bissoon, N

    1997-08-01

    The NMDA receptor has recently been found to be phosphorylated on tyrosine. To assess the possible connection between tyrosine phosphorylation of the NMDA receptor and signaling pathways in the postsynaptic cell, we have investigated the relationship between tyrosine phosphorylation and the binding of NMDA receptor subunits to the SH2 domains of phospholipase C-gamma (PLC-gamma). A glutathione S-transferase (GST) fusion protein containing both the N- and the C-proximal SH2 domains of PLC-gamma was bound to glutathione-agarose and reacted with synaptic junctional proteins and glycoproteins. Tyrosine-phosphorylated PSD-GP180, which has been identified as the NR2B subunit of the NMDA receptor, bound to the SH2-agarose beads in a phosphorylation-dependent fashion. Immunoblot analysis with antibodies specific for individual NMDA receptor subunits showed that both NR2A and NR2B subunits bound to the SH2-agarose. No binding occurred to GST-agarose lacking an associated SH2 domain, indicating that binding was specific for the SH2 domains. The binding of receptor subunits increased after the incubation of synaptic junctions with ATP and decreased after treatment of synaptic junctions with exogenous protein tyrosine phosphatase. Immunoprecipitation experiments confirmed that NR2A and NR2B were phosphorylated on tyrosine and further that tyrosine phosphorylation of each of the subunits was increased after incubation with ATP. The results demonstrate that NMDA receptor subunits NR2A and NR2B will bind to the SH2 domains of PLC-gamma and that isolated synaptic junctions contain endogenous protein tyrosine kinase(s) that can phosphorylate both NR2A and NR2B receptor subunits, and suggest that interaction of the tyrosine-phosphorylated NMDA receptor with proteins that contain SH2 domains may serve to link it to signaling pathways in the postsynaptic cell.

  16. S2k-Leitlinie zum Gebrauch von Präparationen zur lokalen Anwendung auf der Haut (Topika).

    Science.gov (United States)

    Wohlrab, Johannes; Staubach, Petra; Augustin, Matthias; Eisert, Lisa; Hünerbein, Andreas; Nast, Alexander; Reimann, Holger; Strömer, Klaus; Mahler, Vera

    2018-03-01

    Diese Leitlinie richtet sich an Assistenz- und Fachärzte der Dermatologie sowie an Kostenträger und politische Entscheidungsgremien. Die Leitlinie wurde im formellen Konsensusverfahren (S2k) von Dermatologen unter Einbindung von Apothekern erstellt. Die Leitlinie stellt allgemeine Aspekte der Pharmakokinetik sowie der regulatorischen Begrifflichkeiten dar. Es werden Empfehlungen zur Indikation von Magistralrezepturen sowie deren Qualitätssicherung gegeben. Die Bedeutung der galenischen Grundlagen und die Problematik bei einer Substitution gegeneinander verschiedener Grundlagen werden dargestellt. Die Leitlinie umfasst Kriterien zur Auswahl einer adäquaten Grundlage sowie spezifische Aspekte zur Therapieplanung. Die Leitlinie gibt Empfehlungen zum Management bei Unverträglichkeiten gegenüber Bestandteilen der Grundlagen oder Hilfsstoffe. © 2018 The Authors | Journal compilation © Blackwell Verlag GmbH, Berlin.

  17. Der Weg zum Java-Profi Konzepte und Techniken für die professionelle Java-Entwicklung

    CERN Document Server

    Inden, Michael

    2015-01-01

    Sie haben bereits Einiges an Erfahrung mit Java und möchten Ihre Entwicklungstätigkeit nun professionalisieren? Oder sind Sie schon auf dem Weg zum Profi, benötigen aber ein Nachschlagewerk, das Ihnen die wichtigen Themen aus der Java-Welt kompakt und kompetent vermittelt? Dieses Buch bietet eine umfassende Einführung in die professionelle Entwicklung und vermittelt Ihnen das notwendige Wissen, um stabile und erweiterbare Softwaresysteme auf Java-SE-Basis zu bauen. Praxisnahe Beispiele helfen dabei, das Gelernte rasch umzusetzen. Neben der Praxis wird viel Wert auf das Verständnis zugrunde liegender Konzepte gelegt. Dabei kommen dem Autor Michael Inden seine umfangreichen Schulungs- und Entwicklererfahrungen zugute - und Ihnen als Leser damit ebenso. Diese Neuauflage wurde durchgehend überarbeitet, aktualisiert und erweitert. Natürlich darf das aktuelle Java 8 nicht fehlen. Verschiedene Kapitel sind Java 8 und seinen Neuerungen gewidmet. Dort wird ein fundierter Einstieg in die umfangreichen Erweit...

  18. Status seminar on BMBF-funded research projects in ecotoxicology. Proceedings; Statusseminar zum Foerderschwerpunkt `Oekotoxikologie` des BMBF. Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Kirchner, M. [ed.; Bauer, H. [ed.

    1995-07-01

    Research projects in the field of ecotoxicology are presented, with particular regard to aquatic and terrestrial ecotoxicology. The report covers pollutant effects on plancton and fish and pollutant monitoring in streams. The effects, distribution and mobility of PAH, PCB and other pollutants in soils and pollutant concentrations in microorganisms are gone into. (SR) [Deutsch] Vorgestellt werden die Forschungsvorhaben zum Thema Oekotoxikologie. Schwerpunkte hierbei sind die aquatische Oekotoxokologie und die terrestrische Oekotoxikologie. Berichtet wird ueber die Wirkung von Fremdstoffen auf Plankton und Fischen, sowie die Bewertung der Belastung von Fliessgewaessern.Ausserdem wird die Wirkung, Verteilung und die Mobilitaet von PAK, PCB und anderen Schadstoffen in Boeden dargestellt, sowie die Belastung von Mikroorganismen mit Schadstoffen. (SR)

  19. Genome-wide identification of nuclear receptor (NR) superfamily genes in the copepod Tigriopus japonicus.

    Science.gov (United States)

    Hwang, Dae-Sik; Lee, Bo-Young; Kim, Hui-Su; Lee, Min Chul; Kyung, Do-Hyun; Om, Ae-Son; Rhee, Jae-Sung; Lee, Jae-Seong

    2014-11-18

    Nuclear receptors (NRs) are a large superfamily of proteins defined by a DNA-binding domain (DBD) and a ligand-binding domain (LBD). They function as transcriptional regulators to control expression of genes involved in development, homeostasis, and metabolism. The number of NRs differs from species to species, because of gene duplications and/or lineage-specific gene losses during metazoan evolution. Many NRs in arthropods interact with the ecdysteroid hormone and are involved in ecdysone-mediated signaling in arthropods. The nuclear receptor superfamily complement has been reported in several arthropods, including crustaceans, but not in copepods. We identified the entire NR repertoire of the copepod Tigriopus japonicus, which is an important marine model species for ecotoxicology and environmental genomics. Using whole genome and transcriptome sequences, we identified a total of 31 nuclear receptors in the genome of T. japonicus. Nomenclature of the nuclear receptors was determined based on the sequence similarities of the DNA-binding domain (DBD) and ligand-binding domain (LBD). The 7 subfamilies of NRs separate into five major clades (subfamilies NR1, NR2, NR3, NR4, and NR5/6). Although the repertoire of NR members in, T. japonicus was similar to that reported for other arthropods, there was an expansion of the NR1 subfamily in Tigriopus japonicus. The twelve unique nuclear receptors identified in T. japonicus are members of NR1L. This expansion may be a unique lineage-specific feature of crustaceans. Interestingly, E78 and HR83, which are present in other arthropods, were absent from the genomes of T. japonicus and two congeneric copepod species (T. japonicus and Tigriopus californicus), suggesting copepod lineage-specific gene loss. We identified all NR receptors present in the copepod, T. japonicus. Knowledge of the copepod nuclear receptor repertoire will contribute to a better understanding of copepod- and crustacean-specific NR evolution.

  20. Cholinergic cells in the nucleus basalis of mice express the N-methyl-D-aspartate-receptor subunit NR2C and its replacement by the NR2B subunit enhances frontal and amygdaloid acetylcholine levels

    NARCIS (Netherlands)

    De Souza Silva, M. A.; Dolga, Amalia; Pieri, I.; Marchetti, L.; Eisel, U. L. M.; Huston, J. P.; Dere, E.

    2006-01-01

    It is known that glutamatergic and cholinergic systems interact functionally at the level of the cholinergic basal forebrain. The N-methyl-D-aspartate receptor (NMDA-R) is a multiprotein complex composed of NR1, NR2 and/or NR3 subunits. The subunit composition of NMDA-R of cholinergic cells in the

  1. Tubin: Sinfonie nr. 11 (ergänzt von Kaljo Raid) / Christoph Schlüren

    Index Scriptorium Estoniae

    Schlüren, Christoph

    1997-01-01

    Uuest heliplaadist "Tubin: Sinfonie nr. 11 (ergänzt von Kaljo Raid); Pärt: Nekrolog op. 5, Sinfonie nr. 1; Tüür: Searching for Roots, Insula deserta, Zeitraum. Königliches Philharmonisches Orchester Stockholm, Paavo Järvi". Virgin/EMI CD 5 45212 2 (WD:71'34") DDD

  2. Host range expansion and increased damage potential of Euwallacea nr. fornicatus (Coleoptera: Curculionidae) in Florida

    Science.gov (United States)

    Ambrosia beetles in the Euwallacea nr. fornicatus complex (Coleoptera: Curculionidae) vector Fusarium spp. fungi pathogenic to susceptible hosts, including avocado. The Florida avocado production area in Miami-Dade County was surveyed for E. nr. fornicatus upon observations of initial damage in 2016...

  3. The role of linked phospholipids in the rubber-filler interaction in carbon nanotube (CNT) filler natural rubber (NR) composites

    NARCIS (Netherlands)

    Le, H.H.; Abhijeet, S.; Ilish, S.; Klehm, J.; Henning, S.; Beiner, M.; Sarkawi, S.S.; Dierkes, Wilma K.; Das, A.; Fischer, D.; Stöckelhuber, K.-W.; Wiessner, S.; Khatiwada, S.P.; Adhikari, R.; Pham, T.; Heinrich, G.; Radusch, H.-J.

    2014-01-01

    The aim of the present work is to evidence the role of the linked phospholipids of natural rubber (NR) in the rubber-carbon nanotube (CNT) interactions in rubber composites. Three rubbers namely NR, deproteinized NR (DPNR) and a synthetic rubber isoprene (IR) were used as matrix for CNTs. The

  4. "Peer Review: Nonroad (NR) Updates to Population Growth, Compression Ignition (CI) Criteria, Toxic Emission Factors and Speciation Profiles"

    Science.gov (United States)

    This report focuses on the methodology for estimating growth in NR engine populations as used in the MOVES201X-NONROAD emission inventory model. MOVES NR growth rates start with base year engine populations and estimate growth in the populations of NR engines, while applying cons...

  5. Crosslinked bicontinuous biobased PLA/NR blends via dynamic vulcanization using different curing systems.

    Science.gov (United States)

    Yuan, Daosheng; Chen, Kunling; Xu, Chuanhui; Chen, Zhonghua; Chen, Yukun

    2014-11-26

    In this study, blends of entirely biosourced polymers, namely polylactide (PLA) and natural rubber (NR), were prepared through dynamic vulcanization using dicumyl peroxide (DCP), sulphur (S) and phenolic resin (2402) as curing agents, respectively. The crosslinked NR phase was found to be a continuous structure in all the prepared blends. The molecular weight changes of PLA were studied by gel permeation chromatography. Interfacial compatibilization between PLA and NR was investigated using Fourier transform infrared spectroscopy and scanning electron microscopy. The thermal properties of blends were evaluated by differential scanning calorimetry and thermogravimetric analysis instrument. It was found that the molecular weight of PLA and interfacial compatibilizaion between PLA and NR showed a significant influence on the mechanical and thermal properties of blends. The PLA/NR blend (60/40 w/w) by DCP-induced dynamic vulcanization owned the finest mechanical properties and thermal stability. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. „… höher als die Liebe zur Wissenschaft steht die Treue zum eigenen Vaterland …“: Hallenser Romanisten im Ersten Weltkrieg

    Directory of Open Access Journals (Sweden)

    Annette Schiller

    2014-12-01

    Full Text Available Die 1914 verbreitete Kriegsbegeisterung nahm auch die Romanisten nicht aus. Briefe und Tagebuchnotizen aus der Zeit zeigen, wie Lehrende und Studenten unseres Faches, deren gemeinsamer Bezug die Zugehörigkeit zum Romanischen Seminar Halle war, vom „Großen Krieg“ betroffen waren und führen uns die Situation und Geisteshaltung der Professoren und Studenten und die Rückwirkungen des Krieges auf das Fach vor Augen.

  7. Das Unsichtbare sichtbar machen. Zum vergessenen Beitrag der alten deutschen Frauenbewegung zur Wohn- und Siedlungsreform im frühen 20. Jahrhundert

    Directory of Open Access Journals (Sweden)

    Claudia Wucherpfennig

    2007-03-01

    Full Text Available Der Wohnungs- und Städtebau der Moderne gilt weitgehend als eine „Männerdomäne”. In ihrer Schrift Die Wohnungsfrage ist Frauensache! zeigen Ulla Terlinden und Susanna von Oertzen demgegenüber die vielfältigen und einflussreichen Beiträge der alten deutschen Frauenbewegung zur Wohnungsreform des frühen 20. Jahrhunderts und zum Neuen Bauen auf.

  8. Feinkartierung von Loss of Heterozygosity-Intervallen auf Chromosom 11p und Untersuchungen zum Expressions- und Methylierungsstatus von FANCF beim Ovarialkarzinom

    OpenAIRE

    Schulze, Kathrin

    2011-01-01

    Um Tumorsuppressorgene beim Ovarialkarzinom zu identifizieren wurde eine Kartierung von LOH-Intervallen der Region 11p15.5 - 11p11.2 mittels 11 Mikrosatellitenmarkern an 47 Ovarialtumorproben vorgenommen. Im Bereich des ST5- und AFM255/KAI1-Locus konnten potentielle Intervalle abgegrenzt werden. Das Fanconi Anämie Gen FANCF wurde für Untersuchungen zum Expressions- und Methylierungsstatus ausgewählt, da dessen Abschaltung mit einem besseren Ansprechen auf Cisplatin bei Ovarialkarzinompatienti...

  9. Molecular pathways: the role of NR4A orphan nuclear receptors in cancer.

    LENUS (Irish Health Repository)

    Mohan, Helen M

    2012-06-15

    Nuclear receptors are of integral importance in carcinogenesis. Manipulation of classic ligand-activated nuclear receptors, such as estrogen receptor blockade in breast cancer, is an important established cancer therapy. Orphan nuclear receptors, such as nuclear family 4 subgroup A (NR4A) receptors, have no known natural ligand(s). These elusive receptors are increasingly recognized as molecular switches in cell survival and a molecular link between inflammation and cancer. NR4A receptors act as transcription factors, altering expression of downstream genes in apoptosis (Fas-ligand, TRAIL), proliferation, DNA repair, metabolism, cell migration, inflammation (interleukin-8), and angiogenesis (VEGF). NR4A receptors are modulated by multiple cell-signaling pathways, including protein kinase A\\/CREB, NF-κB, phosphoinositide 3-kinase\\/AKT, c-jun-NH(2)-kinase, Wnt, and mitogen-activated protein kinase pathways. NR4A receptor effects are context and tissue specific, influenced by their levels of expression, posttranslational modification, and interaction with other transcription factors (RXR, PPAR-Υ). The subcellular location of NR4A "nuclear receptors" is also important functionally; novel roles have been described in the cytoplasm where NR4A proteins act both indirectly and directly on the mitochondria to promote apoptosis via Bcl-2. NR4A receptors are implicated in a wide variety of malignancies, including breast, lung, colon, bladder, and prostate cancer; glioblastoma multiforme; sarcoma; and acute and\\/or chronic myeloid leukemia. NR4A receptors modulate response to conventional chemotherapy and represent an exciting frontier for chemotherapeutic intervention, as novel agents targeting NR4A receptors have now been developed. This review provides a concise clinical overview of current knowledge of NR4A signaling in cancer and the potential for therapeutic manipulation.

  10. NR2A contributes to genesis and propagation of cortical spreading depression in rats.

    Science.gov (United States)

    Bu, Fan; Du, Ruoxing; Li, Yi; Quinn, John P; Wang, Minyan

    2016-03-22

    Cortical spreading depression (CSD) is a transient propagating excitation of synaptic activity followed by depression, which is implicated in migraine. Increasing evidence points to an essential role of NR2A-containing NMDA receptors in CSD propagation in vitro; however, whether these receptors mediate CSD genesis in vivo requires clarification and the role of NR2A on CSD propagation is still under debate. Using in vivo CSD in rats with electrophysiology and in vitro CSD in chick retina with intrinsic optical imaging, we addressed the role of NR2A in CSD. We demonstrated that NVP-AAM077, a potent antagonist for NR2A-containing receptors, perfused through microdialysis probes, markedly reduced cortex susceptibility to CSD, but also reduced magnitude of CSD genesis in rats. Additionally, NVP-AAM077 at 0.3 nmol perfused into the contralateral ventricle, considerably suppressed the magnitude of CSD propagation wave and propagation rate in rats. This reduction in CSD propagation was also observed with TCN-201, a negative allosteric modulator selective for NR2A, at 3 μM, in the chick retina. Our data provides strong evidence that NR2A subunit contributes to CSD genesis and propagation, suggesting drugs selectively antagonizing NR2A-containing receptors might constitute a highly specific strategy treating CSD associated migraine with a likely better safety profile.

  11. Memory Enhancement by Targeting Cdk5 Regulation of NR2B

    Science.gov (United States)

    Plattner, Florian; Hernandéz, Adan; Kistler, Tara M.; Pozo, Karine; Zhong, Ping; Yuen, Eunice Y.; Tan, Chunfeng; Hawasli, Ammar H.; Cooke, Sam F.; Nishi, Akinori; Guo, Ailan; Wiederhold, Thorsten; Yan, Zhen; Bibb, James A.

    2014-01-01

    SUMMARY Many psychiatric and neurological disorders are characterized by learning and memory deficits, for which cognitive enhancement is considered a valid treatment strategy. The N-methyl-D-aspartate receptor (NMDAR) is a prime target for the development of cognitive enhancers due to its fundamental role in learning and memory. In particular, the NMDAR subunit NR2B improves synaptic plasticity and memory when over-expressed in neurons. However, NR2B regulation is not well understood and no therapies potentiating NMDAR function have been developed. Here, we show that serine 1116 of NR2B is phosphorylated by cyclin-dependent kinase 5 (Cdk5). Cdk5-dependent NR2B phosphorylation is regulated by neuronal activity and controls the receptor’s cell surface expression. Disrupting NR2B-Cdk5 interaction using a small interfering peptide (siP) increases NR2B surface levels, facilitates synaptic transmission, and improves memory formation in vivo. Our results reveal a novel regulatory mechanism critical to NR2B function that can be targeted for the development of cognitive enhancers. PMID:24607229

  12. NR4A1 is an endogenous inhibitor of vocal fold fibrosis.

    Science.gov (United States)

    Hiwatashi, Nao; Bing, Renjie; Kraja, Iv; Branski, Ryan C

    2017-09-01

    NR4A1 was recently identified as an endogenous inhibitor of transforming growth factor (TGF)-β-induced fibrosis, and the role of this nuclear receptor has not been elucidated in tissue health or the response to injury in the vocal folds. Given the clinical implications of vocal fold fibrosis, we investigated NR4A1 expression during vocal fold wound healing in vivo and the regulatory roles of NR4A1 on vocal fold fibroblasts (VFFs) in vitro with the ultimate goal of developing targeted therapies for this challenging patient population. In vivo and in vitro. In vivo, the temporal pattern of NR4A1 mRNA expression was quantified following rat vocal fold injury. In vitro, the role of NR4A1 on TGF-β1-mediated transcription of genes underlying fibrosis as well as myofibroblast differentiation and collagen gel contraction was quantified in our human VFF line. Small interfering RNA was employed to alter NR4A1 expression to further elucidate this complex system. Nr4a1 mRNA increased 1 day after injury and peaked at 7 days. Knockdown of NR4A1 resulted in upregulation of COL1A1 and TGF-β1, with TGF-β1 stimulation (both P vocal fold health or disease. Upregulation of TGF-β following vocal fold injury was concurrent with increased NR4A1 expression. These data provide a foundation for the development of therapeutic strategies given persistent TGF-β signaling in vocal fold fibrosis. N/A Laryngoscope, 127:E317-E323, 2017. © 2017 The American Laryngological, Rhinological and Otological Society, Inc.

  13. A novel NR2E3 gene mutation in autosomal recessive retinitis pigmentosa with cystic maculopathy

    OpenAIRE

    Mahajan, D.; Votruba, Marcela

    2017-01-01

    NR2E3 is a gene that encodes for photoreceptor cell specific nuclear receptor, which is involved in cone proliferation. The splice site mutation 119-2A>C in NR2E3 (15q23) has been previously reported to underlie recessive enhanced cone S sensitivity syndrome, clumped pigmentary retinal degeneration, Goldman-Favre syndrome and also autosomal dominant and autosomal recessive retinitis pigmentosa (RP). However, the mutation c 571 + 2 T > C in NR2E3 has not been previously reported with retinal d...

  14. Pengaruh Penggunaan Nr dan Nbr dengan Bahan Pengisi Silika terhadap Sifat Fisika Kompon Rol Karet Cpo

    OpenAIRE

    Prasetya, Hari Adi

    2010-01-01

    The research aimed to obtain the influence of natural rubber NR, synthetic rubber NBR and alumunium silikat (AISiO₃) as a filler, its physical properties and also to find out rubber compound the best for rubber mixture with variation of NR : NBR 50, 70 : 30, 90 : 10 hr and variation of aluminium silikat (ASiO₂) 45 phr, 50 phr, 55 phr. The result showed that the addition of rubber NR : NBR ratio and alumunium silikat (ASiO₂) had significant effect on the hardness, tensile strength, abration re...

  15. Study of rheological, viscoelastic and vulcanization behavior of sponge EPDM/NR blended nano- composites

    International Nuclear Information System (INIS)

    Bashir, M Arshad; Shahid, M; Ahmed, Riaz; Yahya, A G

    2014-01-01

    In this research paper the effect of blending ratio of natural rubber (NR) with Ethylene Propylene Diene Monomer (EPDM) were investigated. Different samples of EPDM/NR ratio were prepared to study the variation of NR in EPDM on rheology, curing characteristics, tangent δ, and viscosity variation during vulcanization of sponge nano composites.The main aim of present research is to develop elastomeric based sponge composites with the blending ratio of base elastomers along with the carbon nano particles for high energy absorbing and damping applications. The curing characteristics, rheology and viscoelastic nature of the composite is remarkably influenced with the progressive blending ratio of the base elastomeric matrix

  16. Study of rheological, viscoelastic and vulcanization behavior of sponge EPDM/NR blended nano-composites

    International Nuclear Information System (INIS)

    Bashir, M. A.; Shahid, M.; Ahmed, R.; Yahya, A. G.

    2013-01-01

    In this research paper the effect of blending ratio of natural rubber (NR) with Ethylene Propylene Diene Monomer (EPDM) were investigated. Different samples of EPDM/NR ratio were prepared to study the variation of NR in EPDM on rheology, curing characteristics, tangent d, and viscosity variation during vulcanization of sponge nano composites. The main aim of present research is to develop elastomeric based sponge composites with the blending ratio of base elastomers along with the carbon nano particles for high energy absorbing and damping applications. The curing characteristics, rheology and viscoelastic nature of the composite is remarkably influenced with the progressive blending ratio of the base elastomeric matrix. (author)

  17. The mechanical properties of radiation-vulcanized NR/BR blending system

    Energy Technology Data Exchange (ETDEWEB)

    Yan Aoshuang E-mail: yanas@public3.bta.net.cn; Guo Zhengtao; Li Li; Zhai Ying; Zhou Peng

    2002-03-01

    The effect of radiation dose on the mechanical properties of NR/BR blending system is reported in this paper. A comparison was made between sulphur vulcanization and radiation vulcanization for an optimal nature rubber (NR)/ butyl rubber (BR) blending ratio (60/40) at dose range from 10 to 150 kGy. The result shows that the mechanical properties, especially, tensile strength, elongation at break, and tear strength have been improved significantly by radiation-vulcanization. This finding was also proved by thermal aging experiment on a selected NR/BR blend at 70 deg. C for up to 168 h.

  18. Postsynaptic density protein 95-regulated NR2B tyrosine phosphorylation and interactions of Fyn with NR2B in levodopa-induced dyskinesia rat models

    Directory of Open Access Journals (Sweden)

    Ba M

    2014-12-01

    Full Text Available Maowen Ba,1,* Min Kong,2,* Guozhao Ma3 1Department of Neurology, Yuhuangding Hospital, Yantai City, Shandong, People’s Republic of China; 2Department of Neurology, Yantaishan Hospital, Yantai City, Shandong, People’s Republic of China; 3Department of Neurology, Shandong Provincial Qianfoshan Hospital, Shandong University, Shandong, People’s Republic of China *These authors contributed equally to this work Context: Abnormality in interactions between N-methyl-d-aspartate (NMDA receptor and its signaling molecules occurs in the lesioned striatum in Parkinson’s disease (PD and levodopa-induced dyskinesia (LID. It was reported that Fyn-mediated NR2B tyrosine phosphorylation, can enhance NMDA receptor function. Postsynaptic density protein 95 (PSD-95, one of the synapse-associated proteins, regulates interactions between receptor and downstream-signaling molecules. In light of the relationship between PSD-95, NR2B, and Fyn kinases, does PSD-95 contribute to the overactivity of NMDA receptor function induced by dopaminergic treatment? To further prove the possibility, the effects of regulating the PSD-95 expression on the augmented NR2B tyrosine phosphorylation and on the interactions of Fyn and NR2B in LID rat models were evaluated.Methods: In the present study, parkinsonian rat models were established by injecting 6-hydroxydopamine. Subsequently, valid PD rats were treated with levodopa (50 mg/kg/day with benserazide 12.5 mg/kg/day, twice daily intraperitoneally for 22 days to create LID rat models. Then, the effect of pretreatment with an intrastriatal injection of the PSD-95mRNA antisense oligonucleotides (PSD-95 ASO on the rotational response to levodopa challenge was assessed. The effects of pretreatment with an intrastriatal injection of PSD-95 ASO on the augmented NR2B tyrosine phosphorylation and interactions of Fyn with NR2B in the LID rat models were detected by immunoblotting and immunoprecipitation. Results: Levodopa

  19. Enantioselectivity and Thermostability of a Novel Hyperhermotolerant Lipase from Geobacillus Thermodenitrificans nr68 (Lip.nr-68) on Secondary Racemic Alcohols Acetylation

    Science.gov (United States)

    Nik Him, N. R.; Ibrahim, D.

    2018-05-01

    In our previous work, a new lipase enzyme has been purified from a species identified as a Gram negative Geobacillus thermodenitrificans nr68, isolated from a hot spring in Malaysia with growth temperature of 48°C. This new lipase, called Lip.nr-68 has been characterized as a hyperthermotolerant protein with high stability at 65°C and has been showing excellent characteristics that are very much comparable yet better than some of those of well-known industrially-used lipases. It shows high activity against long-chain triglycerides with molecular weight of the purified enzyme estimated to be 33.5 kDa using SDS-PAGE analysis. This paper is focusing on hyperthermotolerant Lip.nr-68 performance in promoting for enantioselectivity activities towards three secondary racemic alcohols namely 1-phenylethanol, 1-cyclohexilethanol and 1-(naft-2-il) ethanol by acetylation with vinyl acetate. Lip.nr-68 has been confirmed to show high and usual enantioselectivitiy according to the Kazlauskas Rule towards all secondary racemic alcohols and has significantly approved as an enantiomer selective biocatalyst towards 1-phenylethanol and 1-cyclohexylethanol at 65°C. Lip.nr-68 has showed a reduction of (R) and (S) enantiomers as well as the production of 68-98% ee and almost 94% yield of 3-4 mg/ml for 1-cyclohexilethanol.

  20. Genome-wide identification of nuclear receptor (NR) genes and the evolutionary significance of the NR1O subfamily in the monogonont rotifer Brachionus spp.

    Science.gov (United States)

    Kim, Duck-Hyun; Kim, Hui-Su; Hwang, Dae-Sik; Kim, Hee-Jin; Hagiwara, Atsushi; Lee, Jae-Seong; Jeong, Chang-Bum

    2017-10-01

    Nuclear receptors (NRs) are a large family of transcription factors that are involved in many fundamental biological processes. NRs are considered to have originated from a common ancestor, and are highly conserved throughout the whole animal taxa. Therefore, the genome-wide identification of NR genes in an animal taxon can provide insight into the evolutionary tendencies of NRs. Here, we identified all the NR genes in the monogonont rotifer Brachionus spp., which are considered an ecologically key species due to their abundance and world-wide distribution. The NR family was composed of 40, 32, 29, and 32 genes in the genomes of the rotifers B. calyciflorus, B. koreanus, B. plicatilis, and B. rotundiformis, respectively, which were classified into seven distinct subfamilies. The composition of each subfamily was highly conserved between species, except for NR1O genes, suggesting that they have undergone sporadic evolutionary processes for adaptation to their different environmental pressures. In addition, despite the dynamics of NR evolution, the significance of the conserved endocrine system, particularly for estrogen receptor (ER)-signaling, in rotifers was discussed on the basis of phylogenetic analyses. The results of this study may help provide a better understanding the evolution of NRs, and expand our knowledge of rotifer endocrine systems. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. ‹Portale›, die zum Nachdenken anregen. Einblicke in eine Interfaceanalyse des Augmented Reality Spiels Ingress

    Directory of Open Access Journals (Sweden)

    Franco Rau

    2017-02-01

    Full Text Available Augmented Reality Spiele wie ‹Ingress› und ‹PokémonGo› stellen aktuell neue mediale Phänomene dar. Eine zentrale Differenz zu klassischen Massively Multiplayer Online Games (MMOs ist die Verzahnung von virtuellen und realen Objekten als konstitutive Spielelemente. In Anknüpfung an die Perspektive, digitale Spiele als Kultur- und Bildungsräume zu verstehen, widmet sich der Beitrag der Frage, inwiefern das Ingress-Spielen die Perspektive von Spieler/innen auf den öffentlichen Raum verändern kann. In erster Annäherung an diese Fragestellung wird auf Basis einer Interfaceanalyse gezeigt, wie ‹Portale› – virtueller Repräsentationen realer Objekte im Spiel – potenziell Irritationen und Differenz­erfahrungen ermöglichen. Diese können zum Ausgangspunkt weiterführender Reflexionen werden. Dafür werden exemplarisch ‹Portale› diskutiert, die (1. weiterführende Informationen zu realen Objekte bieten, die (2. Momentaufnahmen nicht mehr existierender kultureller Objekte darstellen und die (3. reale Objekte in ironischer Weise präsentieren.

  2. Smart market. From smart grid to the intelligent energy market; Smart Market. Vom Smart Grid zum intelligenten Energiemarkt

    Energy Technology Data Exchange (ETDEWEB)

    Aichele, Christian [Hochschule Kaiserslautern, Zweibruecken (Germany). Fachbereich Betriebswirtschaft; Doleski, Oliver D. (ed.)

    2014-07-01

    Dare more market. - The design of this postulate provides an important contribution to the success of the German energy transition. The Bundesnetzagentur has shown with its highly regarded benchmark paper on smart grids and markets leads the way towards more market in the energy sector. The therein required differentiation in a network and market sphere contributes to greater transparency on the consumer side and enables a gid releaving shift in energy consumption. The book focuses on actors and roles in the modified market circumstances as well as components and products of a future Smart Markets. Finally, to the reader concrete business models are offered. Authors from science and practice give in this book answers on how the interaction of Smart Grid and Smart Market works. [German] Mehr Markt wagen. - Die Ausgestaltung dieses Postulats liefert einen wichtigen Beitrag zum Gelingen der deutschen Energiewende. Die Bundesnetzagentur hat mit ihrem vielbeachteten Eckpunktepapier zu intelligenten Netzen und Maerkten diesen Weg in Richtung mehr Markt in der Energiewirtschaft gewiesen. Die darin geforderte Differenzierung in eine Netz- und Marktsphaere traegt zu mehr Transparenz auf der Verbraucherseite bei und ermoeglicht eine netzentlastende Verlagerung des Energieverbrauchs. Das Buch beleuchtet Akteure und Rollen im geaenderten Marktumfeld ebenso wie Komponenten und Produkte eines zukuenftigen Smart Markets. Schliesslich werden dem Leser konkrete Geschaeftsmodelle angeboten. Autoren aus Wissenschaft und Praxis geben in diesem Buch Antworten darauf, wie das Zusammenspiel von Smart Grid und Smart Market funktioniert.

  3. Water winning in aquifers in the catchment area of the Elbe river. Task report no. 5: Radon applied for characterisation of geohydraulic processes; Wassergewinnung in Talgrundwasserleitern im Einzugsgebiet der Elbe. Teilbericht zum Thema Nr. 5: Radon zur Charakterisierung geohydraulischer Prozesse

    Energy Technology Data Exchange (ETDEWEB)

    Dehnert, J.; Nestler, W. [Hochschule fuer Technik und Wirtschaft, Dresden (Germany). Lab. fuer Geotechnik und Wasserwesen; Freyer, K.; Treutler, H.C. [UFZ - Umweltforschungszentrum Leipzig-Halle GmbH, Leipzig (Germany). Sektion Analytik

    1998-06-09

    The work performed has two objectives. The first is to test a new method for determination of optimal volumes of pumped groundwater samples at official sampling and measuring sites, in order to significantly enhance the level of representativity of groundwater samples. The second is to establish information about the required framework conditions which enable natural radon activity concentrations in river bank filtrates to be used for determination of residence times or infiltration velocities, respectively, of infiltration from surface waters to aquifers. Aspects of required equipment and instrumentation for both objectives are explained in the report. (orig./CB) [Deutsch] Mit dieser Arbeit werden zwei Ziele verfolgt. Mit dem neuen Verfahren zur Bestimmung optimaler Abpumpvolumen von Grundwassermessstellen soll die Repraesentativitaet von Grundwasserproben bei der Probennahme deutlich verbessert werden. Das zweite Ziel besteht in der Untersuchung der Voraussetzungen, unter denen die natuerliche Radonaktivitaetskonzentration des Uferfiltrats zur Bestimmung von Aufenthaltszeiten bzw. Infiltrationsgeschwindigkeiten von infiltriertem Oberflaechenwasser in Talgrundwasserleitern genutzt werden kann. Fuer beide Zielstellungen werden Moeglichkeiten fuer eine geraetetechnische Umsetzung aufgezeigt. (orig.)

  4. Orphan nuclear receptor NR4A2 inhibits hepatic stellate cell proliferation through MAPK pathway in liver fibrosis.

    Science.gov (United States)

    Chen, Pengguo; Li, Jie; Huo, Yan; Lu, Jin; Wan, Lili; Li, Bin; Gan, Run; Guo, Cheng

    2015-01-01

    Hepatic stellate cells (HSCs) play a crucial role in liver fibrosis, which is a pathological process characterized by extracellular matrix accumulation. NR4A2 is a nuclear receptor belonging to the NR4A subfamily and vital in regulating cell growth, metabolism, inflammation and other biological functions. However, its role in HSCs is unclear. We analyzed NR4A2 expression in fibrotic liver and stimulated HSCs compared with control group and studied the influence on cell proliferation, cell cycle, cell apoptosis and MAPK pathway after NR4A2 knockdown. NR4A2 expression was examined by real-time polymerase chain reaction, Western blotting, immunohistochemistry and immunofluorescence analyses. NR4A2 expression was significantly lower in fibrotic liver tissues and PDGF BB or TGF-β stimulated HSCs compared with control group. After NR4A2 knockdown α-smooth muscle actin and Col1 expression increased. In addition, NR4A2 silencing led to the promotion of cell proliferation, increase of cell percentage in S phase and reduced phosphorylation of ERK1/2, P38 and JNK in HSCs. These results indicate that NR4A2 can inhibit HSC proliferation through MAPK pathway and decrease extracellular matrix in liver fibrogenesis. NR4A2 may be a promising therapeutic target for liver fibrosis.

  5. Non-genomic effects of the NR4A1/Nur77/TR3/NGFIB orphan nuclear receptor.

    Science.gov (United States)

    Pawlak, Alicja; Strzadala, Leon; Kalas, Wojciech

    2015-03-01

    The orphan nuclear receptor NR4A1/Nur77/TR3/NGFIB acts primarily as a transcription factor to regulate the expression of multiple genes. However, increasing research attention has recently been given to non-genomic activities of NR4A1. The first description of a non-genomic action of NR4A1 referred to the conversion of anti-apoptotic Bcl-2 into a pro-apoptotic protein by direct interaction with NR4A1. In response to certain apoptotic stimuli, NR4A1 translocates from the nucleus to the mitochondrial outer membrane (MOM) where it associates with Bcl-2 and thereby causes apoptosis. Afterwards, it appeared that NR4A1 could also bind and convert other anti-apoptotic Bcl-2 family members. The latest studies indicate a significant role of NR4A1 in the process of autophagy. For example, a new NR4A1-mediated pathway specific for melanoma cells has been described where NR4A1 interacts with the adenine nucleotide translocase 1 (ANT1) on the mitochondrial inner membrane (MIM) leading to induction of the autophagy pathway. Moreover, NR4A1 interaction with cytoplasmic p53 may also contribute to the induction of autophagy. In addition to mitochondria, NR4A1 could be translocated to the outer membrane of the endoplasmic reticulum (ER) and associate with Bcl-2 or translocon-associated protein subunit γ (TRAPγ) causing ER stress-induced apoptosis. NR4A1 also contributes to the proteasomal degradation of β-catenin in colon cancer cells in vitro and in vivo, as well as to the stabilization of hypoxia-inducible factor-1α (HIF-1α) under non-hypoxic conditions. This review summarizes research findings on non-genomic effects of NR4A1 in normal and cancer cells. Copyright © 2015 Elsevier Inc. All rights reserved.

  6. Objekt nr 2011 / Liina Siib, Signe Kivi, Vilen Künnapu ...[jt.

    Index Scriptorium Estoniae

    2011-01-01

    Installatsioon "Objekt nr. 2011" Tallinnas Vabaduse väljakul 27.02.-20.03.2011. Arvamust avaldavad kuplis objektiks olnud. Installatsiooni autorid: Mart Koldits, Kaarel Oja, Iir Hermeliin, Indrek Tiigi, Kadri Tamre, Kadri Tikerpuu, Sirli Bergström

  7. The LANL C-NR counting room and fission product yields

    Energy Technology Data Exchange (ETDEWEB)

    Jackman, Kevin Richard [Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

    2015-09-21

    This PowerPoint presentation focused on the following areas: LANL C-NR counting room; Fission product yields; Los Alamos Neutron wheel experiments; Recent experiments ad NCERC; and Post-detonation nuclear forensics

  8. Kalinnikov: Sinfonie Nr.2 A-Dur, Ouvertüre Zar Boris / Hanspeter Krellmann

    Index Scriptorium Estoniae

    Krellmann, Hanspeter

    1990-01-01

    Uuest heliplaadist "Kalinnikov: Sinfonie Nr.2 A-Dur, Ouvertüre Zar Boris, Die Zeder und die Palme. Scottish National Orchestra, Neeme Järvi" (AD: 1989) Chandos / Koch Records CD 8805 (WD: 59'40") DDD

  9. Dynamic aperture of NR-2000 in the work regime with a small radiation emittance

    International Nuclear Information System (INIS)

    Zelinskij, A.Yu.; Karnaukhov, I.M.; Kononenko, S.G.; Tarasenko, A.S.

    1991-01-01

    NR-2000 is an electron storage ring installation with 6-pole magnets compensating its chromatism. The results of NR-2000 dynamic aperture numerical modelling in 6-dimension space are given. Perturbation of the equilibrium orbit and that of magnetic field up to the third order of magnitude were taken into consideration. Calculations were made according to DeCA programme. 7 refs.; 5 figs. (author)

  10. iNR-Drug: predicting the interaction of drugs with nuclear receptors in cellular networking.

    Science.gov (United States)

    Fan, Yue-Nong; Xiao, Xuan; Min, Jian-Liang; Chou, Kuo-Chen

    2014-03-19

    Nuclear receptors (NRs) are closely associated with various major diseases such as cancer, diabetes, inflammatory disease, and osteoporosis. Therefore, NRs have become a frequent target for drug development. During the process of developing drugs against these diseases by targeting NRs, we are often facing a problem: Given a NR and chemical compound, can we identify whether they are really in interaction with each other in a cell? To address this problem, a predictor called "iNR-Drug" was developed. In the predictor, the drug compound concerned was formulated by a 256-D (dimensional) vector derived from its molecular fingerprint, and the NR by a 500-D vector formed by incorporating its sequential evolution information and physicochemical features into the general form of pseudo amino acid composition, and the prediction engine was operated by the SVM (support vector machine) algorithm. Compared with the existing prediction methods in this area, iNR-Drug not only can yield a higher success rate, but is also featured by a user-friendly web-server established at http://www.jci-bioinfo.cn/iNR-Drug/, which is particularly useful for most experimental scientists to obtain their desired data in a timely manner. It is anticipated that the iNR-Drug server may become a useful high throughput tool for both basic research and drug development, and that the current approach may be easily extended to study the interactions of drug with other targets as well.

  11. iNR-Drug: Predicting the Interaction of Drugs with Nuclear Receptors in Cellular Networking

    Directory of Open Access Journals (Sweden)

    Yue-Nong Fan

    2014-03-01

    Full Text Available Nuclear receptors (NRs are closely associated with various major diseases such as cancer, diabetes, inflammatory disease, and osteoporosis. Therefore, NRs have become a frequent target for drug development. During the process of developing drugs against these diseases by targeting NRs, we are often facing a problem: Given a NR and chemical compound, can we identify whether they are really in interaction with each other in a cell? To address this problem, a predictor called “iNR-Drug” was developed. In the predictor, the drug compound concerned was formulated by a 256-D (dimensional vector derived from its molecular fingerprint, and the NR by a 500-D vector formed by incorporating its sequential evolution information and physicochemical features into the general form of pseudo amino acid composition, and the prediction engine was operated by the SVM (support vector machine algorithm. Compared with the existing prediction methods in this area, iNR-Drug not only can yield a higher success rate, but is also featured by a user-friendly web-server established at http://www.jci-bioinfo.cn/iNR-Drug/, which is particularly useful for most experimental scientists to obtain their desired data in a timely manner. It is anticipated that the iNR-Drug server may become a useful high throughput tool for both basic research and drug development, and that the current approach may be easily extended to study the interactions of drug with other targets as well.

  12. NMDAR NR2A and NR2B specific PKC-dependent regulation of mGluR is defective in the Fragile X Syndrome mouse model

    DEFF Research Database (Denmark)

    Banke, Tue G.; Toft, Anna Karina; Lundbye, Camilla Johanne

    The Fragile X Syndrome (FXS) animal model, the Fmr1 knock-out (KO) mouse, has demonstrated an increased mGluR5-mediated long-term depression (LTD). However, surprisingly little information exists about other ion channels/receptors and their effects on FXS, including NMDA receptors (NMDAR). Here we....... Furthermore, in this model it appears that NR2B activation stimulates PKC, while NR2A activation halts or reverses this effect. In addition, in the KO mice, the coupling between specific NMDAR subunits and mGluR-LTD activity through PKC seems defective in an age-dependent manner. These findings suggest strong...

  13. Discussion of the applicability of overdammed fixed-bed reactors in nitrification processes; Beitrag zum Einsatz von ueberstauten Festbettreaktoren zur Nitrifikation

    Energy Technology Data Exchange (ETDEWEB)

    Guenter, H.O.

    1996-12-31

    The investigation aimed at developing a dimensioning method for overdammed fixed bed nitrification reactors with plug flow which takes account of the reactor height. Further, information was to be obtained for assessing sudden loads and for comparing energy consumption with the data of conventional activated sludge plants. (orig./SR) [Deutsch] Ziel dieser Arbeit ist es, fuer ueberstaute, pfropfendurchstroemte Festbettreaktoren zur Nitrifikation einen Bemessungsansatz aufzustellen, welcher den Einfluss der Reaktorhoehe mit beruecksichtigt. Ferner sollten Erkenntnisse zur Beurteilung von Stossbelastungen sowie zum Energiebedarf im Vergleich zu herkoemmlichen Belebungsanlagen gewonnen werden. (orig./SR)

  14. Multimedia CD-Rom on hydrogen and fuel cells. 2. rev. ed.; Die Multimedia CD-Rom zum Thema Wasserstoff und Brennstoffzellen

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2007-07-01

    This CD-Rom contains well-presented information with informative illustrations, as well as a large download section with publications, reports, and presentations in.pdf form for in-depth studies. There is also a list of links to selected websites, with short explanations. [German] Die CD zum Thema ''Wasserstoff und Brennstoffzellen'' bietet sowohl gut gegliederte Informationen mit anschaulichen Bildern fuer die Lektuere am Monitor als auch eine grosse Download-Rubrik, in der zahlreiche Publikationen, Fachberichte, Praesentationen als PDF-Dateien abgelegt sind und fuers Studium bereitstehen. Ausserdem gibt es ein Link-Verzeichnis mit Verweisen und Erlaeuterungen zu ausgewaehlten Internet-Seiten.

  15. Die ILO-Standards zum Schutz von Arbeitnehmerrechten : ein Begründungsversuch mit Hilfe der kantischen Position von Norman E. Bowie

    OpenAIRE

    Bäumlisberger, Damian; Brink, Alexander

    2012-01-01

    "Der Beitrag ergründet die normativen Grundlagen der Standards zum Schutz der Arbeitnehmerrechte der International Labour Organization. Auf Basis der kantischen Position des US-amerikanischen Wirtschaftsethikers Norman E. Bowie zeigen die Autoren, dass sich Bowies Theorieansatz grundsätzlich dazu eignet, die vier Kernbereiche der ILO-Aktivitäten ethisch zu fundieren. Jedoch stößt Bowies Position bei der Begründung der ILO-Standards auch auf Grenzen, die nur teilweise mittels zusätzlicher, mit...

  16. The Value of Serum NR2 Antibody in Prediction of Post-Cardiopulmonary Resuscitation Survival

    Directory of Open Access Journals (Sweden)

    Ali Bidari

    2015-07-01

    Full Text Available Introduction: N-methyl-D-aspartate receptor subunits antibody (NR2-ab is a sensitive marker of ischemic brain damage in clinical circumstances, such as cerebrovascular accidents. We aimed to assess the value of serum NR2-ab in predicting the post-cardiopulmonary resuscitation (CPR survival. Methods: In this cohort study, we examined serum NR2-ab levels 1 hour after the return of spontaneous circulation (ROSC in 49 successfully resuscitated patients. Patients with traumatic or asphyxic arrests, prior neurological insults, or major medical illnesses were excluded. Participants were followed until death or hospital discharge. Demographic data, coronary artery disease risk factors, time before initiation of CPR, and CPR duration were documented.  In addition, Glasgow coma scale (GCS, blood pressure, and survival status of patients were recorded at 1, 6, 24, and 72 hour(s after ROSC. Descriptive analyses were performed, and the Cox proportional hazard model was applied to assess if NR2-ab level is an independent predictive factor of survival. Results: 49 successfully resuscitated patients were evaluated; 27 (55% survived to hospital discharge, 4 (8.1% were in vegetative state, 10 (20.4% were physically disabled, and 13 (26.5% were physically functional. Within 72 hours of ROSC all of the 12 NR2-ab positive patients died. In contrast, 31 (84% of the NR2-ab negative patients survived. Sensitivity, specificity, positive and negative likelihood ratios of NR2-ab in prediction of survival were 54.5% (95%CI=32.7%-74.9%, 100% (95%CI=84.5%-100%, infinite, and 45.5% (95%CI=28.8%-71.8%, respectively. Subsequent analysis showed that both NR2-ab status and GCS were independent risk factors of death. Conclusions: A positive NR2-ab serum test 1 hour after ROSC correlated with lower 72-hour survival. Further studies are required to validate this finding and demonstrate the value of a quantitative NR2-ab assay and its optimal time of measurement.

  17. Fluxes of total reactive atmospheric nitrogen (ΣNr using eddy covariance above arable land

    Directory of Open Access Journals (Sweden)

    Christophe R. Flechard

    2013-02-01

    Full Text Available The amount and timing of reactive nitrogen exchange between agricultural land and the atmosphere play a key role in evaluating ecosystem productivity and in addressing atmospheric nitrogen budgets and transport. With the recent development of the Total Reactive Atmospheric Nitrogen Converter (TRANC apparatus, a methodology has been provided for continuous measurement of the sum of all airborne nitrogen containing species (ΣNr allowing for diurnal and seasonal investigations. We present ΣNr concentration and net flux data from an 11-month field campaign conducted at an arable field using the TRANC system within an eddy-covariance setup. Clear diurnal patterns of both ΣNr concentrations and fluxes with significant dependencies on atmospheric stability and stomatal regulation were observed in the growing season. TRANC data were compared with monthly-averaged concentrations and dry deposition rates of selected Nr compounds using DELTA denuders and ensemble-averages of four inferential models, respectively. Similar seasonal trends were found for Nr concentrations from DELTA and TRANC measurements with values from the latter being considerably higher than those of DELTA denuders. The variability of the difference between these two systems could be explained by seasonally changing source locations of NOx contributions to the TRANC signal. As soil and vegetation Nr emissions to the atmosphere are generally not treated by inferential (dry deposition models, TRANC data showed lower monthly deposition rates than those obtained from inferential modelling. Net ΣNr exchange was almost neutral (~0.072 kg N ha−1 at the end of the observation period. However, during most parts of the year, slight but permanent net ΣNr deposition was found. Our measurements demonstrate that fertilizer addition followed by substantial ΣNr emissions plays a crucial role in a site's annual atmospheric nitrogen budget. As long-term Nr measurements with high temporal

  18. Dose management in radiology. Review of the technological status; Dosismanagement in der Radiologie. Ein Ueberblick zum Stand der Technik

    Energy Technology Data Exchange (ETDEWEB)

    Verius, M. [LKH Innsbruck, Universitaetsklinik fuer Radiologie, Innsbruck (Austria)

    2015-08-15

    The Euratom directive 2013/59 (''EU directive for radiation protection'') has to be implemented into national law by spring 2018 and requires a complete recording of patient dosages and relevant parameters. Additionally, a medical physics expert has to be consulted for each radiological examination above a defined threshold. A complete recording of the dosage administered from all modalities and optimization of the radiological procedures should result in a reduction of the total dosage. This can be achieved by automated systems that incorporate not only the detection of the dose parameters but also the evaluation and analysis of these data. When provided with warning levels such a system should be able to inform or warn the operator when dose thresholds have been exceeded or even better inform the operator about possible excess dosages before an examination. Depending on the information provided by the modality, dose management systems can operate at different levels in the picture archiving and communication system (PACS), radiological and hospital information systems (RIS/HIS) or with the header information of a digital imaging and communications in medicine (DICOM) image and evaluate and analyze this data. A practicable use of such systems is only possible by close cooperation of medical personnel, medical physicists and information technology (IT) administrators. Various systems are available commercially or free but an individual adaptation of these systems is useful and necessary, depending on the requirements of the radiology practice or hospital. (orig.) [German] Die Euratom-Richtlinie 2013/59 (''EU-Richtlinie zum Strahlenschutz'') besagt, dass sie bis zum Fruehjahr 2018 in nationales Recht der Mitgliedstaaten umgesetzt werden muss, d. h. eine lueckenlose Erfassung der Patientendosen zu erfolgen hat. Ausserdem muss zu jeder Modalitaet in einem bestimmten Ausmass ein Medizinphysikexperte hinzugezogen werden. Die

  19. Training to NDT construction experts (ZFPBau). Developments in recent years; Ausbildung zum ZfPBau-Experten. Entwicklungen der letzten Jahre

    Energy Technology Data Exchange (ETDEWEB)

    Taffe, Alexander [Hochschule fuer Technik und Wirtschaft, Berlin (Germany); Feistkorn, Sascha [SVTI Schweizerischer Verein fuer technische Inspektionen, Wallisellen (Switzerland). Nuklearinspektorat; Dauberschmidt, Christoph [Hochschule Muenchen (Germany)

    2016-05-01

    Classical destructive test methods are fixed part of industrial sectors for decades such as aerospace, automotive, railway and power plants. A high degree of standardization of procedures, and training in form of an ISO standard are present, but not in non-destructive testing of civil engineering (ZfPBau). Except for the rebound hammer NDT methods in civil engineering are not standardized. Also of personnel qualifications can be found in regulations very few requirements, although it is obvious that this is an indispensable prerequisite for the proper application of the procedures and reliable inspection results. In this contribution experiences with a presented training concept in which the construction inspector will trained to DIN 1076 in a two-day course. Here are the necessary conditions worked out, such as the creation of suitable test bodies or the definition of necessary course content for establishing training courses. [German] Klassische zerstoerungsfreie Pruefverfahren sind seit Jahrzehnten fester Bestandteil industrieller Bereiche wie Luftfahrt, Automotive, Eisenbahn und Kraftwerke. Ein hoher Normungsgrad der Verfahren und Ausbildungsstandards in Form einer ISO-Norm liegen vor. Bei der zerstoerungsfreien Pruefung im Bauwesen (ZfPBau) ist das anders. Bis auf den Rueckprallhammer sind ZfPBau-Verfahren nicht genormt. Auch zur Personalqualifikation finden sich in Regelwerken nur sehr wenige Anforderungen, obwohl es offensichtlich ist, dass dies eine unverzichtbare Voraussetzung fuer die richtige Anwendung der Verfahren und zuverlaessige Pruefaussagen ist. In diesem Beitrag werden Erfahrungen mit einem Ausbildungskonzept vorgestellt, bei dem Bauwerkspruefer nach DIN 1076 in einem zweitaegigen Lehrgang ausgebildet werden. Dabei werden die notwendigen Voraussetzungen heraus gearbeitet, wie z.B. die Erstellung von geeigneten Testkoerpern oder die Festlegung notwendiger Kursinhalte, die zum Etablieren von Ausbildungskursen erforderlich sind.

  20. Futurelab Medienpädagogik: Qualitätsentwicklung – Professionalisierung – Standards. Thesenpapier zum Forum Kommunikationskultur 2017 der GMK

    Directory of Open Access Journals (Sweden)

    Thomas Knaus

    2017-10-01

    Full Text Available Das Forum Kommunikationskultur der Gesellschaft für Medienpädagogik und Kommunikationskultur (GMK steht im Jahr 2017 unter dem Anspruch, sich mit Fragen der Qualitätsentwicklung medienpädagogischen Handelns in Wissenschaft und Praxis auseinanderzusetzen. Dabei soll besonderes Augenmerk auf Fragen der Professionalisierung und der Entwicklung von Standards gelegt werden. Zu diesen Fragen stellen wir im Folgenden ausgewählte Thesen mit ergänzenden und erweiternden Erläuterungen im Sinne eines programmatischen Textes zur Diskussion. Die mit diesem Papier anzustossende Diskussion soll zunächst einer konstruktiv-weiterführenden internen Klärung wichtiger Fragen medienpädagogischer Praxis und Forschung dienen und dabei auch anzeigen, welche Positionen in der Community konsensfähig sind und welche möglicherweise strittig erscheinen. Zugleich sollen die Thesen und die Diskussion zu einer Positionsbestimmung der GMK bezüglich praktischer und wissenschaftlicher medienpädagogischer Arbeit nach aussen beitragen. Mit der Diskussion ist das Ziel verbunden, künftige Bedarfe für Handlungs- und Forschungsfelder der Medienpädagogik – jedoch ohne Anspruch auf Vollständigkeit – zu identifizieren. Um der Prägnanz und Kürze willen, wird der zu jeder These gehörende Hintergrund nur knapp angedeutet, ohne die damit verbundenen (teils sehr umfassenden Diskurse im Detail nachzuzeichnen. Angegebene Literaturbezüge sollen jeweils beispielhaft auf differenzierende und tiefergehende Ausführungen verweisen. Die Thesenfolge beginnt mit Überlegungen zum technikinduzierten gesellschaftlichen Wandel („Digitalisierung“ und seiner Bedeutung für die Medienbildung. Dies vorausschickend, gehen wir auf Fragen der Qualifizierung und Professionalisierung für praktisches Handeln sowie auf die Rolle der Medienpädagogik als Wissenschaft und schliessend auf den Stellenwert von Standards zur Orientierung und Reflexion ein.

  1. Chronic zinc exposure decreases the surface expression of NR2A-containing NMDA receptors in cultured hippocampal neurons.

    Directory of Open Access Journals (Sweden)

    Jia Zhu

    Full Text Available Zinc distributes widely in the central nervous system, especially in the hippocampus, amygdala and cortex. The dynamic balance of zinc is critical for neuronal functions. Zinc modulates the activity of N-methyl-D-aspartate receptors (NMDARs through the direct inhibition and various intracellular signaling pathways. Abnormal NMDAR activities have been implicated in the aetiology of many brain diseases. Sustained zinc accumulation in the extracellular fluid is known to link to pathological conditions. However, the mechanism linking this chronic zinc exposure and NMDAR dysfunction is poorly understood.We reported that chronic zinc exposure reduced the numbers of NR1 and NR2A clusters in cultured hippocampal pyramidal neurons. Whole-cell and synaptic NR2A-mediated currents also decreased. By contrast, zinc did not affect NR2B, suggesting that chronic zinc exposure specifically influences NR2A-containg NMDARs. Surface biotinylation indicated that zinc exposure attenuated the membrane expression of NR1 and NR2A, which might arise from to the dissociation of the NR2A-PSD-95-Src complex.Chronic zinc exposure perturbs the interaction of NR2A to PSD-95 and causes the disorder of NMDARs in hippocampal neurons, suggesting a novel action of zinc distinct from its acute effects on NMDAR activity.

  2. Determination of Crosslink Concentration by Mooney-Rivlin Equation for Vulcanized NR/ SBR Blend and its Influence on Mechanical Properties

    International Nuclear Information System (INIS)

    Azreen Izzati Dzulkifli; Che Mohd Som Said; Han, C.C.

    2015-01-01

    Crosslink concentration is an important property affecting the major characteristic of cured rubber. The crosslink concentration was determined using Mooney-Rivlin equation due to its simple and reliable method. Cured natural rubber and styrene butadiene rubber blend (NR/SBR) with different crosslink concentrations were obtained with different blend ratios of 100/0, 80/20, 70/30, 60/40, 50/50, 40/60 and 0/100. The crosslink concentrations were determined using Mooney-Rivlin Equation and its influence on International Rubber Hardness Tester (IRHD), tensile strength and rebound resilience of NR/ SBR blend vulcanizates was investigated. The results showed different blend ratios had an influence on the crosslink concentration of the NR/ SBR blend vulcanizates. Obtained data showed that high NR content in NR/ SBR blend increased the crosslink concentration. The highest crosslink concentration recorded was for 100/0 blend ratio which was 0.0498 mol kg"-"1 RH while the lowest was 0.0295 mol kg"-"1 RH for 0/100 blend ratio. The study on the influence of crosslink concentration on IRHD, tensile strength and rebound resilience of NR/ SBR blend vulcanizates showed that the mechanical properties increased linearly with the crosslink concentration. High NR content in NR/ SBR blends resulted in higher crosslink concentration which improved the performance of mechanical properties for NR/ SBR blend. (author)

  3. A dual system formed by the ARC and NR molybdoenzymes mediates nitrite-dependent NO production in Chlamydomonas.

    Science.gov (United States)

    Chamizo-Ampudia, Alejandro; Sanz-Luque, Emanuel; Llamas, Ángel; Ocaña-Calahorro, Francisco; Mariscal, Vicente; Carreras, Alfonso; Barroso, Juan B; Galván, Aurora; Fernández, Emilio

    2016-10-01

    Nitric oxide (NO) is a relevant signal molecule involved in many plant processes. However, the mechanisms and proteins responsible for its synthesis are scarcely known. In most photosynthetic organisms NO synthases have not been identified, and Nitrate Reductase (NR) has been proposed as the main enzymatic NO source, a process that in vitro is also catalysed by other molybdoenzymes. By studying transcriptional regulation, enzyme approaches, activity assays with in vitro purified proteins and in vivo and in vitro NO determinations, we have addressed the role of NR and Amidoxime Reducing Component (ARC) in the NO synthesis process. N\\R and ARC were intimately related both at transcriptional and activity level. Thus, arc mutants showed high NIA1 (NR gene) expression and NR activity. Conversely, mutants without active NR displayed an increased ARC expression in nitrite medium. Our results with nia1 and arc mutants and with purified enzymes support that ARC catalyses the NO production from nitrite taking electrons from NR and not from Cytb5-1/Cytb5-Reductase, the component partners previously described for ARC (proposed as NOFNiR, Nitric Oxide-Forming Nitrite Reductase). This NR-ARC dual system would be able to produce NO in the presence of nitrate, condition under which NR is unable to do it. © 2016 John Wiley & Sons Ltd.

  4. Thermodynamic analysis of a fuel-cell-system for automotive transportation; Thermodynamische Analyse eines Brennstoffzellensystems zum Antrieb von Kraftfahrzeugen

    Energy Technology Data Exchange (ETDEWEB)

    Berger, Oliver

    2009-09-28

    inclination angle profile of the Grossglockner mountain is followed on the test stand with a speed of 55 km/h without overheating of the cooling system. After optimization of the cooler design and positioning, the new vehicle was able to meet the specifications while the first of the two vehicles only achieved 35 km/h without overheating of the cooling system. Finally, a theoretical investigation identified the measures that should be taken to achieve sufficient cooling in countries with hot climates, e.g. the USA. It was found that it would be sufficient to raise the coolant temperature from 90 C to about 110 C to ensure good performance in these climate zones. (orig.) [German] Ziel der vorliegenden Arbeit ist die thermodynamische Analyse und Optimierung eines Brennstoffzellensystems zum Antrieb von Kraftfahrzeugen. Der Fokus ist im Wesentlichen auf die Kuehlung des Brennstoffzellenstapelmoduls gelegt, wobei aufgrund der Vielzahl an Schnittstellen zwischen der Kuehlung und den anderen Teilsystemen (Wasserstoff- und Luftversorgung) auch eine ganzheitlichere Betrachtung erforderlich ist. Ausgangspunkt der Untersuchungen ist ein bestehendes Brennstoffzellenfahrzeug, anhand dessen in einem ersten Schritt experimentelle Untersuchungen auf Systempruefstaenden und im Klimawindkanal durchgefuehrt werden. Die gewonnenen Messdaten dienen zum einen der Darstellung des Ist-Zustands und zur Ermittlung des Grenzbereichs der Waermeabfuhr an die Umgebung ueber das Brennstoffzellen-Kuehlsystem. Zum anderen werden sie zur Validierung eines dynamischen Kuehlsystemsimulationsmodells herangezogen. Mittels dieses Simulationsmodells werden anschliessend im Rahmen von Sensitivitaetsanalysen die wesentlichen Einflussfaktoren zur Steigerung der Waermeabfuhr an die Umgebung ermittelt. Die daraufhin durchgefuehrten Optimierungen betreffen die Verschaltung der Systemkomponenten sowie deren Auslegung und Platzierung im Fahrzeugvorderwagen. Auf Basis dieser und weiterer Erkenntnisse wird ein weiteres

  5. Pharmacological isolation of postsynaptic currents mediated by NR2A- and NR2B-containing NMDA receptors in the anterior cingulate cortex

    Directory of Open Access Journals (Sweden)

    Cao Xiaoyan

    2007-04-01

    Full Text Available Abstract NMDA receptors (NMDARs are involved in excitatory synaptic transmission and plasticity associated with a variety of brain functions, from memory formation to chronic pain. Subunit-selective antagonists for NMDARs provide powerful tools to dissect NMDAR functions in neuronal activities. Recently developed antagonist for NR2A-containing receptors, NVP-AAM007, triggered debates on its selectivity and involvement of the NMDAR subunits in bi-directional synaptic plasticity. Here, we re-examined the pharmacological properties of NMDARs in the anterior cingulate cortex (ACC using NVP-AAM007 as well as ifenprodil, a selective antagonist for NR2B-containing NMDARs. By alternating sequence of drug application and examining different concentrations of NVP-AAM007, we found that the presence of NVP-AAM007 did not significantly affect the effect of ifenprodil on NMDAR-mediated EPSCs. These results suggest that NVP-AAM007 shows great preference for NR2A subunit and could be used as a selective antagonist for NR2A-containing NMDARs in the ACC.

  6. Mechanical & morphological properties of attapulgite/NR composites: Effect of mixing time variation

    Energy Technology Data Exchange (ETDEWEB)

    Nor, Nor Aina Mohd, E-mail: ayena90@yahoo.com; Othman, Nadras, E-mail: srnadras@usm.my; Ismail, Hanafi, E-mail: ihanafi@usm.my [School of Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia, Seri Ampangan, 14300 Nibong Tebal, Pulau Pinang (Malaysia)

    2015-07-22

    The development of composite material based on attapulgite clay (ATP) as a filler and natural rubber (NR) matrices were prepared by combination of melt mixing and latex compounding methods. Sonication technique was chosen in this work to disperse the attapulgite suspension. 6 phr of attapulgite loading was fabricated using different time of mixing ranging from 30 minutes until 2 hours and sonication time was kept constant at 15 minutes. Then, co-coagulating HA latex with attapulgite clay suspension through latex compounding method produced the masterbatch. The masterbatch was compounded with natural rubber by melt mixing method. The mechanical and morphological characteristics were investigated in this work. From mechanical testing, M1 showed the highest value of tensile and tear strength. By comparing with M30 and M2, M1 shows high 300% tensile modulus and lower crosslink density. However, when the time of mixing was prolonged to 2 hours, the results for tensile strength, elongation at break and tear strength were decreased. This is due to flocculation of attapulgite particles. Sonication techniques also proved that the tensile strength and elongation at break of these three samples were higher compared to gum NR (NR) and attapulgite compounded with NR using a conventional method (in-situ 6). From field emission scanning electron microscope (FESEM) results, it revealed that M1 had good dispersion in the NR system. It is proved that the higher tensile strength was due to good dispersion of attapulgite clay in the NR matrix. It was also supported from crosslink density, which is lower than NR and in-situ 6 results. It showed that the penetration of toluene solvent into rubber compound was restricted. The optimum time, M1 give the best results, which can be compared to control the sample.

  7. Effect of organo clay on curing, mechanical and dielectric properties of NR/SBR blends

    Science.gov (United States)

    Ravikumar, K.; Joseph, Reji; Ravichandran, K.

    2018-04-01

    Natural rubber (NR) and styrene butadiene rubber (SBR) based elastomeric blends reinforced with organically modified Sodium bentonite clay were prepared by two roll mills. Vulcanization parameters such as minimum and maximum torque values scorch and cure times are measured by Oscillating Disc Rheometer. Mechanical properties such as Tensile strength, modulus at 100%, 200% and 300% elongation and elongation at break and Hardness were measured by Universal testing machine and Durometer Shore A hardness meter respectively. Dielectric properties such as dielectric constant (ε’), dissipation factor (tanδ) and volume resistivity (ρv) were measured at room temperature. The curing studies show that torque values are increasing in NR/SBR blends by increase NR content. The scorch and optimum cure time in NR/SBR blends reinforced organo modified clay was found through increase in the SBR content. This may be due to better processing safety of the NR/SBR blends reinforced with organo modified clay. Mechanical properties show that addition of SBR in blends, tensile strength, elongation modulus increases, but 100% modulus slightly increases and no change was observed in Hardness. Dielectric studies show that dielectric constant of NR and SBR rubbers are almost same, it may due to their non-polar nature. But addition of SBR in NR/SBR blend, dielectric constant gradually increases and maximum value observed at 50/50 ratio. But no considerable change was observed in dissipation factor. Frequency dependant resistivity shows that volume resistivity was not changed with respect to frequency up to 3.5 kHz and beyond that the frequency dependence resistivity was found.

  8. Mechanical & morphological properties of attapulgite/NR composites: Effect of mixing time variation

    International Nuclear Information System (INIS)

    Nor, Nor Aina Mohd; Othman, Nadras; Ismail, Hanafi

    2015-01-01

    The development of composite material based on attapulgite clay (ATP) as a filler and natural rubber (NR) matrices were prepared by combination of melt mixing and latex compounding methods. Sonication technique was chosen in this work to disperse the attapulgite suspension. 6 phr of attapulgite loading was fabricated using different time of mixing ranging from 30 minutes until 2 hours and sonication time was kept constant at 15 minutes. Then, co-coagulating HA latex with attapulgite clay suspension through latex compounding method produced the masterbatch. The masterbatch was compounded with natural rubber by melt mixing method. The mechanical and morphological characteristics were investigated in this work. From mechanical testing, M1 showed the highest value of tensile and tear strength. By comparing with M30 and M2, M1 shows high 300% tensile modulus and lower crosslink density. However, when the time of mixing was prolonged to 2 hours, the results for tensile strength, elongation at break and tear strength were decreased. This is due to flocculation of attapulgite particles. Sonication techniques also proved that the tensile strength and elongation at break of these three samples were higher compared to gum NR (NR) and attapulgite compounded with NR using a conventional method (in-situ 6). From field emission scanning electron microscope (FESEM) results, it revealed that M1 had good dispersion in the NR system. It is proved that the higher tensile strength was due to good dispersion of attapulgite clay in the NR matrix. It was also supported from crosslink density, which is lower than NR and in-situ 6 results. It showed that the penetration of toluene solvent into rubber compound was restricted. The optimum time, M1 give the best results, which can be compared to control the sample

  9. Pilot scale experiments on radiation vulcanization of NR latex

    Science.gov (United States)

    Ridwan, M.

    The potential of irradiated latex as raw material of commercial use is under testing on pilot plant scale in Indonesia which has 225 kCi Co-60 irradiation facility and can irradiate 1000 tonnes of centrifuged latex per annum. The facility was jointly designed by BATAN of Indonesia and JAERI of Japan and was jointly financed by UNDP/IAEA, Government of Japan and Government of Indonesia under UNDP/IAEA Regional Cooperative Agreement Project on Industrial Application of Isotopes and Radiation Technology. The facility is a water pool type and can accomodate 400 kCi Co-60. The Co-60 rack has two shapes, plate and cylindrical shapes. The plate shape source is used for natural rubber latex irradiation and the cylindrical one is used for other irradiation services. The vulcanization system consists of three major components : emulsification unit ( height : 650 mm, diameter 500 mm ), mixing unit ( height : 1900mm, diameter 1200 mm ) and vulcanization reactor ( height : 1800 mm, diameter 1300 mm ). The first two components are located outside shielded room while the third one-in irradiation room. The radiation vulcanization process is a much simpler energy saving process comparedto the conventional thermal process which has two vulcanization steps before and after dipping. The physical and mechanical properties of irradiated NR Latex are comparable to those of sulfur vulcanized, and depend on many factors such as irradiation dose, sensitizer content, dry rubber content and storage time.

  10. Pilot scale experiments on radiation vulcanization of NR latex

    International Nuclear Information System (INIS)

    Ridwan, M.

    1985-01-01

    The potential of irradiated latex as raw material of commercial use is under testing on pilot plant scale in Indonesia which has 225 kCi Co-60 irradiation facility and can irradiate 1000 tonnes of centrifuged latex per annum. The facility was jointly designed by BATAN of Indonesia and JAERI of Japan and was jointly financed by UNDP/IAEA, Government of Japan and Government of Indonesia under UNDP/IAEA Regional Cooperative Agreement Project on Industrial Application of Isotopes and Radiation Technology. The facility is a water pool type and can accommodate 400 kCi Co-60. The Co-60 rack has two shapes, plate and cylindrical shapes. The plate shape source is used for natural rubber latex irradiation and the cylindrical one is used for other irradiation services. The vulcanization system consists of three major components: emulsification unit, mixing unit and vulcanization reactor. The first two components are located outside shielded room while the third one in irradiation room. The radiation vulcanization process is a much simpler energy saving process compared to the conventional thermal process which has two vulcanization steps before and after dipping. The physical and mechanical properties of irradiated NR latex are comparable to those of sulfur vulcanized. (author)

  11. Foucault und das Recht: Besprechung von: Christian Schauer, Aufforderung zum Spiel. Foucault und das Recht, Böhlau (Weimar, Köln, Wien 2006, 383 Seiten.

    Directory of Open Access Journals (Sweden)

    Thomas Biebricher

    2007-04-01

    Full Text Available Der Autor rekonstruiert Foucaults Werk zwischen den Jahren 1961 und 1975 und integriert hierbei Perspektiven anderer Autoren (Derrida, Luhmann und Nietzsche, welche zu verwandten Themenkreisen arbeiten und/oder Foucaults Herangehensweise geprägt haben. Eigentliches Ziel der Arbeit ist es, Foucaults Beitrag zur Rechtsgeschichte und –soziologie zu beleuchten. Diese Thematik ist im deutschen Sprachraum bis jetzt kaum systematisch untersucht worden, und dementsprechend gebührt Schauers Studie besondere Beachtung. Der Autor betont Foucaults Ausführungen zum Verhältnis zwischen Recht und Psychiatrie. Dessen Diagnose hebt die “Kolonisierung” des Rechts durch forensisch psychiatrische Diskurse hervor, und Schauer gelingt es, die problematische Dimension dieser Tendenz mit Hinweis auf die schweizerische Rechtspraxis plausibel zu machen. Fluchtpunkt dieser Entwicklung wäre ein bis zum Extrem “vorbeugendes” Recht, das Gefahrenprävention und Risikoregulierung durch präemptive Verhaltensregulierung den Vorzug vor dem Legalitätsprinzip gewährt.

  12. [Dennis Hormuth. Livonia est omnis divisa in partes tres : Studien zum mental mapping der livländischen Chronistik in der Frühen Neuzeit (1558-1721)] / Anti Selart

    Index Scriptorium Estoniae

    Selart, Anti, 1973-

    2013-01-01

    Arvustus: Hormuth, Dennis. Livonia est omnis divisa in partes tres : Studien zum mental mapping der livländischen Chronistik in der Frühen Neuzeit (1558-1721) (Quellen und Studien zur Geschichte des östlichen Europa, 79). Franz Steiner verlag. Stuttgart 2012

  13. Identification of NR4A2 as a transcriptional activator of IL-8 expression in human inflammatory arthritis.

    LENUS (Irish Health Repository)

    Aherne, Carol M

    2009-10-01

    Expression of the orphan nuclear receptor NR4A2 is controlled by pro-inflammatory mediators, suggesting that NR4A2 may contribute to pathological processes in the inflammatory lesion. This study identifies the chemoattractant protein, interleukin 8 (IL-8\\/CXCL8), as a molecular target of NR4A2 in human inflammatory arthritis and examines the mechanism through which NR4A2 modulates IL-8 expression. In TNF-alpha-activated human synoviocyte cells, enhanced expression of IL-8 mRNA and protein correspond to temporal changes in NR4A2 transcription and nuclear distribution. Ectopic expression of NR4A2 leads to robust changes in endogenous IL-8 mRNA levels and co-treatment with TNF-alpha results in significant (p<0.001) secretion of IL-8 protein. Transcriptional effects of NR4A2 on the human IL-8 promoter are enhanced in the presence of TNF-alpha, suggesting molecular crosstalk between TNF-alpha signalling and NR4A2. A dominant negative IkappaB kinase antagonizes the combined effects of NR4A2 and TNF-alpha on IL-8 promoter activity. Co-expression of NR4A2 and the p65 subunit of NF-kappaB enhances IL-8 transcription and functional studies indicate that transactivation occurs independently of NR4A2 binding to DNA or heterodimerization with additional nuclear receptors. The IL-8 minimal promoter region is sufficient to support NR4A2 and NF-kappaB\\/p65 co-operative activity and NR4A2 can interact with NF-kappaB\\/p65 on a 39bp sequence within this region. In patients treated with methotrexate for active inflammatory arthritis, a reduction in NR4A2 synovial tissue levels correlate significantly (n=10, r=0.73, p=0.002) with changes in IL-8 expression. Collectively, these data delineate an important role for NR4A2 in modulating IL-8 expression and reveal novel transcriptional responses to TNF-alpha in human inflammatory joint disease.

  14. Selective up-regulation of NMDA-NR1 receptor expression in myenteric plexus after TNBS induced colitis in rats

    Directory of Open Access Journals (Sweden)

    Price Donald D

    2006-01-01

    Full Text Available Abstract Background N-methyl-D-aspartic acid (NMDA spinal cord receptors play an important role in the development of hyperalgesia following inflammation. It is unclear, however, if changes in NMDA subunit receptor gene expression in the colonic myenteric plexus are associated with colonic inflammation. We investigated regulation of NMDA-NR1 receptor gene expression in TNBS induced colitis in rats. Male Sprague-Dawley rats (150 g–250 g were treated with 20 mg trinitrobenzene sulfonic acid (TNBS diluted in 50% ethanol. The agents were delivered with a 24 gauge catheter inserted into the lumen of the colon. The animals were sacrificed at 2, 7, 14, 21, and 28 days after induction of the colitis, their descending colon was retrieved for reverse transcription-polymerase chain reaction; a subset of animals' distal colon was used for two-dimensional (2-D western analysis and immunocytochemistry. Results NR1-exon 5 (N1 and NR1-exon 21 (C1 appeared 14, 21 and 28 days after TNBS treatment. NR1 pan mRNA was up-regulated at 14, 21, and 28 days. The NR1-exon 22 (C2 mRNA did not show significant changes. Using 2-D western analysis, untreated control rats were found to express only NR1001 whereas TNBS treated rats expressed NR1001, NR1011, and NR1111. Immunocytochemistry demonstrated NR1-N1 and NR1-C1 to be present in the myenteric plexus of TNBS treated rats. Conclusion These results suggest a role for colonic myenteric plexus NMDA receptors in the development of neuronal plasticity and visceral hypersensitivity in the colon. Up-regulation of NMDA receptor subunits may reflect part of the basis for chronic visceral hypersensitivity in conditions such as post-infectious irritable bowel syndrome.

  15. genetic overexpression of NR2B subunit enhances social recognition memory for different strains and species.

    Science.gov (United States)

    Jacobs, Stephanie A; Tsien, Joe Z

    2012-01-01

    The ability to learn and remember conspecifics is essential for the establishment and maintenance of social groups. Many animals, including humans, primates and rodents, depend on stable social relationships for survival. Social learning and social recognition have become emerging areas of interest for neuroscientists but are still not well understood. It has been established that several hormones play a role in the modulation of social recognition including estrogen, oxytocin and arginine vasopression. Relatively few studies have investigated how social recognition might be improved or enhanced. In this study, we investigate the role of the NMDA receptor in social recognition memory, specifically the consequences of altering the ratio of the NR2B:NR2A subunits in the forebrain regions in social behavior. We produced transgenic mice in which the NR2B subunit of the NMDA receptor was overexpressed postnatally in the excitatory neurons of the forebrain areas including the cortex, amygdala and hippocampus. We investigated the ability of both our transgenic animals and their wild-type littermate to learn and remember juvenile conspecifics using both 1-hr and 24-hr memory tests. Our experiments show that the wild-type animals and NR2B transgenic mice preformed similarly in the 1-hr test. However, transgenic mice showed better performances in 24-hr tests of recognizing animals of a different strain or animals of a different species. We conclude that NR2B overexpression in the forebrain enhances social recognition memory for different strains and animal species.

  16. Stenhammer, Wilhelm. sinfonien Nr. 1 F-Dur und Nr. 2 g-Moll op. 34, Excelsior! op. 13, Serenade F-Dur op. 31. Sinfonieorchester Göteborg, Neeme Järvi / Andreas K. W. Meyer

    Index Scriptorium Estoniae

    Meyer, Andreas K. W.

    1995-01-01

    Uuest heliplaadist "Stenhammer, Wilhelm. sinfonien Nr. 1 F-Dur und Nr. 2 g-Moll op. 34, Excelsior! op. 13, Serenade F-Dur op. 31. Sinfonieorchester Göteborg, Neeme Järvi". DG 2 CD 445857-2 (WD: 138'37")

  17. Microstructure, molecular weight and thermal behavior of natural rubber (NR) from mangabeira (Hancornia speciosa)

    International Nuclear Information System (INIS)

    Santos, Expedito Flavio dos; Feitosa, Judith P.A.; Ricardo, Nagila M.P.S.

    2001-01-01

    The natural rubber (NR) from Hancornia speciosa contains characteristics that turns it a good alternative in elastomers supply. The NMR and IR spectra showed that the rubber of mangabeira is composed fundamentally by poly(1,4-cis-isoprene). The rubber molecular weight obtained by GPC and viscometry was 2,0x10 6 and 1,3x10 6 g/mol, respectively, in good agreement with the values determined for seringueira and manicoba NR. The glass transition temperature obtained by DSC (Tg = - 65 deg C) showed the mangabeira rubber is ideal to be utilized in regions of cold climate without compromising its mechanical properties. The rubber has also good thermal stability up to 213 deg C, as indicated by TG curves. This results indicated that the mangabeira NR can be effectively used in vulcanized articles or to be added to asphalt. (author)

  18. Effect of maleic anhydride on the physico-mechanical properties of NR/PE blends

    International Nuclear Information System (INIS)

    Yehia, A.A.; El Elnashar, D.

    2005-01-01

    Blending of two or more polymers is considered as a new technique to produce new materials with new properties at low production cost and investment. Rubber / Rubber blends are well known in tire industry. In the last decade rubber and plastic blending attract the interest of many researchers and technologists. In the present work NR and LLDPE was blended in presence of maleic anhydride (MA) on a Brabender premixed at different conditions and namely temperature and time. The obtained blends were cured with sulphur and peroxide curing systems. Peroxide can crosslink both NR and PE, but the sulphur system crosslinks only the rubber phase in the blend. The data showed also that the addition of MA greatly improved the physico-mechanical properties of NR/PE blends. The surface morphology of the blends under investigation was studied by SEM. The results will be presented and discussed in detail

  19. Distinct expression of synaptic NR2A and NR2B in the central nervous system and impaired morphine tolerance and physical dependence in mice deficient in postsynaptic density-93 protein

    Directory of Open Access Journals (Sweden)

    Johns Roger A

    2008-10-01

    Full Text Available Abstract Postsynaptic density (PSD-93, a neuronal scaffolding protein, binds to and clusters N-methyl-D-aspartate receptor (NMDAR subunits NR2A and NR2B at cellular membranes in vitro. However, the roles of PSD-93 in synaptic NR2A and NR2B targeting in the central nervous system and NMDAR-dependent physiologic and pathologic processes are still unclear. We report here that PSD-93 deficiency significantly decreased the amount of NR2A and NR2B in the synaptosomal membrane fractions derived from spinal cord dorsal horn and forebrain cortex but did not change their levels in the total soluble fraction from either region. However, PSD-93 deficiency did not markedly change the amounts of NR2A and NR2B in either synaptosomal or total soluble fractions from cerebellum. In mice deficient in PSD-93, morphine dose-dependent curve failed to shift significantly rightward as it did in wild type (WT mice after acute and chronic morphine challenge. Unlike WT mice, PSD-93 knockout mice also showed marked losses of NMDAR-dependent morphine analgesic tolerance and associated abnormal sensitivity in response to mechanical, noxious thermal, and formalin-induced inflammatory stimuli after repeated morphine injection. In addition, PSD-93 knockout mice displayed dramatic loss of jumping activity, a typical NMDAR-mediated morphine withdrawal abstinence behavior. These findings indicate that impaired NMDAR-dependent neuronal plasticity following repeated morphine injection in PSD-93 knockout mice is attributed to PSD-93 deletion-induced alterations of synaptic NR2A and NR2B expression in dorsal horn and forebrain cortex neurons. The selective effect of PSD-93 deletion on synaptic NMDAR expression in these two major pain-related regions might provide the better strategies for the prevention and treatment of opioid tolerance and physical dependence.

  20. Mutations in the DNA-binding domain of NR2E3 affect in vivo dimerization and interaction with CRX.

    Directory of Open Access Journals (Sweden)

    Raphael Roduit

    Full Text Available BACKGROUND: NR2E3 (PNR is an orphan nuclear receptor essential for proper photoreceptor determination and differentiation. In humans, mutations in NR2E3 have been associated with the recessively inherited enhanced short wavelength sensitive (S- cone syndrome (ESCS and, more recently, with autosomal dominant retinitis pigmentosa (adRP. NR2E3 acts as a suppressor of the cone generation program in late mitotic retinal progenitor cells. In adult rod photoreceptors, NR2E3 represses cone-specific gene expression and acts in concert with the transcription factors CRX and NRL to activate rod-specific genes. NR2E3 and CRX have been shown to physically interact in vitro through their respective DNA-binding domains (DBD. The DBD also contributes to homo- and heterodimerization of nuclear receptors. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed NR2E3 homodimerization and NR2E3/CRX complex formation in an in vivo situation by Bioluminescence Resonance Energy Transfer (BRET(2. NR2E3 wild-type protein formed homodimers in transiently transfected HEK293T cells. NR2E3 homodimerization was impaired in presence of disease-causing mutations in the DBD, except for the p.R76Q and p.R104W mutant proteins. Strikingly, the adRP-linked p.G56R mutant protein interacted with CRX with a similar efficiency to that of NR2E3 wild-type and p.R311Q proteins. In contrast, all other NR2E3 DBD-mutant proteins did not interact with CRX. The p.G56R mutant protein was also more effective in abolishing the potentiation of rhodospin gene transactivation by the NR2E3 wild-type protein. In addition, the p.G56R mutant enhanced the transrepression of the M- and S-opsin promoter, while all other NR2E3 DBD-mutants did not. CONCLUSIONS/SIGNIFICANCE: These results suggest different disease mechanisms in adRP- and ESCS-patients carrying NR2E3 mutations. Titration of CRX by the p.G56R mutant protein acting as a repressor in trans may account for the severe clinical phenotype in adRP patients.

  1. Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development

    Science.gov (United States)

    Nissim, Sahar; Weeks, Olivia; Talbot, Jared C.; Hedgepeth, John W.; Wucherpfennig, Julia; Schatzman-Bone, Stephanie; Swinburne, Ian; Cortes, Mauricio; Alexa, Kristen; Megason, Sean; North, Trista E.; Amacher, Sharon L.; Goessling, Wolfram

    2016-01-01

    The stepwise progression of common endoderm progenitors into differentiated liver and pancreas organs is regulated by a dynamic array of signals that are not well understood. The nuclear receptor subfamily 5, group A, member 2 gene nr5a2, also known as Liver receptor homolog-1 (Lrh-1) is expressed in several tissues including the developing liver and pancreas. Here, we interrogate the role of Nr5a2 at multiple developmental stages using genetic and chemical approaches and uncover novel pleiotropic requirements during zebrafish liver and pancreas development. Zygotic loss of nr5a2 in a targeted genetic null mutant disrupted the development of the exocrine pancreas and liver, while leaving the endocrine pancreas intact. Loss of nr5a2 abrogated exocrine pancreas markers such as trypsin, while pancreas progenitors marked by ptf1a or pdx1 remained unaffected, suggesting a role for Nr5a2 in regulating pancreatic acinar cell differentiation. In the developing liver, Nr5a2 regulates hepatic progenitor outgrowth and differentiation, as nr5a2 mutants exhibited reduced hepatoblast markers hnf4α and prox1 as well as differentiated hepatocyte marker fabp10a. Through the first in vivo use of Nr5a2 chemical antagonist Cpd3, the iterative requirement for Nr5a2 for exocrine pancreas and liver differentiation was temporally elucidated: chemical inhibition of Nr5a2 function during hepatopancreas progenitor specification was sufficient to disrupt exocrine pancreas formation and enhance the size of the embryonic liver, suggesting that Nr5a2 regulates hepatic versus pancreatic progenitor fate choice. Chemical inhibition of Nr5a2 at a later time during pancreas and liver differentiation was sufficient to block the formation of mature acinar cells and hepatocytes. These findings define critical iterative and pleiotropic roles for Nr5a2 at distinct stages of pancreas and liver organogenesis, and provide novel perspectives for interpreting the role of Nr5a2 in disease. PMID:27474396

  2. Exprese NR1 podjednotky NMDA receptoru v animálním modelu schizofrenie

    Czech Academy of Sciences Publication Activity Database

    Šťastný, František; Kozmiková, I.; Klaschka, Jan; Peková, S.; Tejkalová, H.; Vrajová, M.

    2006-01-01

    Roč. 10, Suppl. 3 (2006), s. 12-15 ISSN 1211-7579 R&D Projects: GA MŠk(CZ) 1M0517; GA MZd NF7626; GA MZd NR8797 Institutional research plan: CEZ:AV0Z10300504; CEZ:AV0Z50110509 Keywords : schizofrenie * NMDA receptor * exprese NR1 podjednotky * animál ní model * kyselina chinolinová * prepulzní inhibice akustického úletu Subject RIV: FL - Psychiatry, Sexuology

  3. Overvåking av jordboende sopp i Røsskleiva NR, Bamble 2016

    OpenAIRE

    Brandrud, Tor Erik; Dima, Bàlint

    2017-01-01

    Brandrud, T.E. & Dima, B. 2017. Overvåking av jordboende sopp i Røsskleiva NR, Bamble 2016. – NINA Kortrapport 80. 15 s. Kartlegging (start av overvåking) av habitat-spesifikke, jordboende kalksopper i nordre del av Røsskleiva NR ble gjennomført i 2016, før oppstart av skjøtselstiltak med storfébeiting. Tilsammen 27 habitat-spesifikke arter, inkludert 18 rødlistede arter ble registrert i løpet av to registreringsrunder i 2016. Funnene fordelte seg på 10 kalkbarskogsopper, 5 kalklinde-skogs...

  4. Pharmacological Activators of the NR4A Nuclear Receptors Enhance LTP in a CREB/CBP-Dependent Manner.

    Science.gov (United States)

    Bridi, Morgan S; Hawk, Joshua D; Chatterjee, Snehajyoti; Safe, Stephen; Abel, Ted

    2017-05-01

    Nr4a nuclear receptors contribute to long-term memory formation and are required for long-term memory enhancement by a class of broad-acting drugs known as histone deacetylase (HDAC) inhibitors. Understanding the molecular mechanisms that regulate these genes and identifying ways to increase their activity may provide novel therapeutic approaches for ameliorating cognitive dysfunction. In the present study, we find that Nr4a gene expression after learning requires the cAMP-response element binding (CREB) interaction domain of the histone acetyltransferase CREB-binding protein (CBP). These gene expression deficits emerge at a time after learning marked by promoter histone acetylation in wild-type mice. Further, mutation of the CREB-CBP interaction domain reduces Nr4a promoter acetylation after learning. As memory enhancement by HDAC inhibitors requires CREB-CBP interaction and Nr4a gene function, these data support the notion that the balance of histone acetylation at the Nr4a promoters is critical for memory formation. NR4A ligands have recently been described, but the effect of these drugs on synaptic plasticity or memory has not been investigated. We find that the 'C-DIM' NR4A ligands, para-phenyl substituted di-indolylmethane compounds, enhance long-term contextual fear memory and increase the duration of long-term potentiation (LTP), a form of hippocampal synaptic plasticity. LTP enhancement by these drugs is eliminated in mice expressing a dominant negative form of NR4A and attenuated in mice with mutation of the CREB-CBP interaction domain. These data define the molecular connection between histone acetylation and Nr4a gene expression after learning. In addition, they suggest that NR4A-activating C-DIM compounds may serve as a potent and selective means to enhance memory and synaptic plasticity.

  5. Technical and economic aspects of fuel cell applications in space HVAC systems; Technische und wirtschaftliche Aspekte zum Einsatz von Brennstoffzellen in der Gebaeudeversorgung

    Energy Technology Data Exchange (ETDEWEB)

    Handke, J; Handschin, E

    1999-12-31

    There are two different types of fuel cells for small-scale applications, li.e. polymer electrolyte membrane fuel cells (PEMFC) and high-termperature fuel cells with ceramic solid electrolytes (SOFC). Both types of fuel cells have different advantages and shortcomings. One of the main problems, however, is the fact that research is still going on and that commercial fuel cell systems for space HVAC applications are still not available. (orig.) [Deutsch] Nach dem heutigen Stand werden zwei unterschiedliche Brennstoffzellen-Typen zum Einsatz in Kleinanlagen entwickelt. Neben der zu Klasse der Niedertemperatur-Brennstoffzellen gehoerenden Polymer-Elektrolyt-Membran-Brennstoffzelle (PEMFC) ist dies die Hochtemperatur-Brennstoffzelle auf der Basis eines keramischen Festelektrolyten (SOFC). Beide Brennstoffzellen-Typen haben unterschiedliche Vor- und Nachteile fuer den Einsatz zur Gebaeudeversorgung. Eines der Hauptprobleme, das allerdings beide Zellentypen gemeinsam haben, besteht in der noch nicht abgeschlossenen Entwicklung und - daraus resultierend - der fehlenden kommerziellen Verfuegbarkeit im Bereich der Gebaeudeversorgung. (orig./MM)

  6. Symposium Ecological construction - from half-timbered- to passive house: living yesterday - today - tomorrow; Symposium Oekologische Bauweisen - vom Umgebinde- zum Passivhaus: Wohnen Gestern - Heute - Morgen

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-07-01

    While the first part of the symposium was dedicated to half-timbered buildings in the traditional style of Lausitz, Bohemia and Silesia, the second part discussed passive buildings. [German] Das Umgebindehaus ist ein weltweit einmaliger Haustyp und zum anderen ist es ein traditionelles Haus unserer Region der Oberlausitz und angrenzender Teile von Boehmen und Schlesien. Das Symposium behandelt das Umgebindehaus in Vergangenheit und Gegenwart und will Motivation und Ansaetze geben, dieses wunderschoene Baukulturgut zu erhalten und unter modernen Wohn-, Arbeits- und Lebensbedingungen zu nutzen. Der zweite Teil der Tagung ist nicht minder wichtig. Beim Passivhaus geht es im wesentlichen darum, Heizwaermeverluste im Winter so klein zu halten, dass sie von den Gewinnen (Sonnenenergie, inneres Waermeaufkommen u.a.) gedeckt werden koennen. Damit wird der Waermeschutz des Gebaeudes vielleicht bis an die Grenzen getrieben und es entstehen neue Anforderungen an das Energiemanagement und die Qualitaet der technischen Gebaeudeausruestung. (orig.)

  7. Proceedings on the seminar on small hydroelectric installations: Practice report and current development; Beitraege zum Seminar Kleinwasserkraft: Praxis und aktuelle Entwicklung

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-31

    This seminar on small hydroelectric installations informs on aspects of construction and administrative aspects. But above all, questions of machinery are dealt with. The book gives an overview of turbine types, topical recent developments in the sector of small hydroelectric power stations, and modern opportunities for fast and inexpensive design of power station components. Beyond the discussion of technical issues, the seminar wants to further contacts between science, turbine manufacturers, consultants and plant operators. (orig.) [Deutsch] Das Seminar Kleinwasserkraft gibt eine Uebersicht ueber bauliche Gestaltung und behoerdliche Aspekte. Vorallem behandelt es aber maschinenbauliche Problemstellungen. Es zeigt einen Ueberblick ueber Turbinentypen, aktuelle Neuentwicklungen im Kleinwassersektor, sowie moderne Moeglichkeiten zum schnellen und preiswerten Design von Kraftwerkskomponenten. Neben dem technischen Teil dient das Seminar auch der Foerderung des Kontakts zwischen Wissenschaft, Turbinenherstellern, Consultants und Anlagenbetreibern. (orig.)

  8. The risk for cancer and genetic abnormalities after radioiodine treatment of hyperthyroidism; Zum Krebs- und genetischen Risiko nach Radioiodtherapie der Hyperthyreose

    Energy Technology Data Exchange (ETDEWEB)

    Reiners, C. [Wuerzburg Univ. (Germany). Klinik und Poliklinik fuer Nuklearmedizin

    1997-12-01

    According to recent studies, the risk for thyroid cancer is not increased after radioiodine treatment in patients with hyperthyroidism. Only the risk of cancer of the stomach seems to be increased slightly in patents treated with I-131 because of functional autonomy. However, the risk for gastric cancer is not increased after higher activities of I-131 because of thyroid cancer. There is no increased risk for genetic abnormalities after radioiodine treatment of hyperthyroidism. (orig.) [Deutsch] Aktuelle Studien zum Karzinomrisiko nach Radioiodtherapie wegen Hyperthyreose ergeben keinen Anhalt fuer eine erhoehte Inzidenz des Schilddruesenkarzinoms nach therapeutischer Gabe von I-131. Allenfalls scheint die Inzidenz von Magenkarzinomen bei Patienten nach Radioiodtherapie wegen funktioneller Autonomie geringfuegig erhoeht zu sein, obwohl diese Beobachtung bei Patienten nach hochdosierter Radioiodtherapie wegen eines Schilddruesenkarzinoms nicht gemacht wurde. Hinweise fuer ein erhoehtes genetisches Risiko nach Radioiodtherapie der Hyperthyreose ergeben sich nicht. (orig.)

  9. Overexpression of the NR2A subunit in the forebrain impairs long-term social recognition and non-social olfactory memory.

    Science.gov (United States)

    Jacobs, S A; Tsien, J Z

    2014-04-01

    Animals must recognize and remember conspecifics and potential mates, and distinguish these animals from potential heterospecific competitors and predators. Despite its necessity, aged animals are known to exhibit impaired social recognition memory. As the brain ages, the ratio of NR2A:NR2B in the brain increases over time and has been postulated to underlie the cognitive decline observed during the aging process. Here, we test the hypothesis that an increased NR2A:NR2B subunit ratio underlies long-term social recognition memory. Using transgenic overexpression of NR2A in the forebrain regions, we investigated the ability of these mice to learn and remember male and female conspecifics, mice of another strain and animals of another rodent species, the rat. Furthermore, due to the importance of olfaction in social recognition, we tested the olfactory memory in the NR2A transgenic mice. Our series of behavioral experiments revealed significant impairments in the NR2A transgenic mice in long-term social memory of both male and female conspecifics. Additionally, the NR2A transgenic mice are unable to recognize mice of another strain or rats. The NR2A transgenic mice also exhibited long-term memory impairments in the olfactory recognition task. Taken together, our results provide evidence that an increased NR2A:NR2B ratio in the forebrain leads to reduced long-term memory function, including the ethologically important memories such as social recognition and olfactory memory.

  10. Recommendations for the use of bioindicators in the frame of Environmetnal Impact Assessment (EIA); Empfehlungen zum Einsatz von Bioindikationsverfahren im Rahmen des Umweltvertraeglichkeitspruefungsgesetzes (UVPG)

    Energy Technology Data Exchange (ETDEWEB)

    Zimmermann, R.D. [Gesellschaft Deutscher Chemiker (GDCh) (Germany). Arbeitskreis Bioindikation]|[Fachhochschule Bingen (Germany). Fachbereich Umweltschutz; Debus, R. [Fachhochschule Wiesbaden (Germany). Fachbereich MND - Umwelttechnik; Franzaring, J. [AB-DLO Wageningen (Netherlands); Hoepker, K.A. [Landesanstalt fuer Umweltschutz, Karlsruhe (Germany); Maier, W. [TUEV Ecoplan Umwelt GmbH, Filderstadt (Germany); Reiml, D. [TUEV Ecoplan Umwelt GmbH, Muenchen (Germany); Finck, M. [Verein Deutscher Ingenieure (VDI) - Kommission Reinhaltung der Luft (RdL), Duesseldorf (Germany)

    1998-11-01

    The paragraph 2 of the German law on Environmental Impact Assessment (law on EIA) requires to determine, to record and to assess the effects which proposed major projects in the areas of industry, local government and transport have on man, animals, plants, soil, water, air, climate, landspace, assets and cultural heritage including the interactions between different media. For this purpose, bioindication is an effective tool. Up to now, the different methods of bioindication were not applied sufficiently although some of the methods have already been standardised in guidelines (VDI, DIN). Users and authorities might feel insecure which bioindication method should be applied when assessing the impacts of a given project. This paper gives recommendations which bioindication method should be applied for the different major projects listed in the law on EIA. A widespread set of data obtained by the use of bioindicators is necessary to meet the demands of paragraph 2. (orig.) [Deutsch] Das Umweltvertraeglichkeitspruefungsgesetz (UVPG) verlangt in Paragraph 2 die Ermittlung, Beschreibung und Bewertung der Auswirkungen eines Vorhabens auf Menschen, Tiere und Pflanzen, Boden, Wasser, Luft, Klima und Landschaft einschliesslich der jeweiligen Wechselwirkungen sowie auf Kultur- und sonstige Sachgueter. Die Bioindikation kann hier wesentliche Dienste leisten. Bislang kamen die verschiedenen, z.T. in Richtlinien (VDI, DIN) vereinheitlichten Verfahren noch zu selten zum Einsatz. Ein Grund hierfuer duerfte in der Unsicherheit der Anwender sowie der Genehmigungsbehoerden liegen, welches Bioindikationsverfahren fuer welches UVPG-Vorhaben eine geeignete Erfassungs- und Bewertungsmethode erlaubt. Zu diesem Zweck werden fuer ausgewaehlte, gemaess UVPG genehmigungsbeduerftige Vorhaben Empfehlungen zum Einsatz von Bioindikationsverfahren erteilt. Erst durch zahlreiche Untersuchungsergebnisse aus diesen Verfahren koennen die Forderungen nach Paragraph 2 UVPG aussagekraeftig erfuellt

  11. Development of radiotracers for imaging NR2B subtype NMDA receptors with positron emission tomography

    International Nuclear Information System (INIS)

    Labas, R.

    2007-01-01

    The aim of this thesis was to develop new radioactive tracers for imaging NR2B subtype NMDA receptors with positron emission tomography. Several compounds including 4-(4-fluoro-benzyl)piperidine and presenting interesting in vivo biological properties were the object of a labelling with a positrons emitter atom ( 11 C or 18 F)

  12. Friction and wear study of NR/SBR blends with Si3N4Filler

    Science.gov (United States)

    GaneshKumar, A.; Balaganesan, G.; Sivakumar, M. S.

    2018-04-01

    The aim of this paper is to investigate mechanical and frictional properties of natural rubber/styrene butadiene rubber (NR/SBR) blends with and without silicon nitride (Si3N4) filler. The rubber is surface modified by silane coupling agent (Si-69) for enhancing hydrophobic property. The Si3N4of percentage 0 1, 3, 5 and 7, is incorporated into NR/SBR rubber compounds with 20% precipitated silica. The specimens with and without fillers are prepared as per standard for tensile and friction testing. Fourier transform infrared (FTIR) spectroscopy test is conducted and it is inferred that the coupling agent is covalently bonded on the surface of Si3N4 particles and an organic coating layer is formed. The co-efficient of friction and specific wear rate of NR/SBR blends are examined using an in-house built friction tester in a disc-on-plate (DOP) configuration. The specimens are tested to find coefficient of friction (COF) against steel grip antiskid plate under dry, mud, wet and oil environmental conditions. It is found that the increase in tensile strength and modulus at low percentage of Si3N4 dispersion. It is also observed that increase in sliding friction co-efficient and decrease in wear rate for 1% of Si3N4 dispersion in NR/SBR blends. The friction tested surfaces are inspected using Scanning Electron Microscope (SEM) and 3D non contact surface profiler.

  13. ESR investigation of NR and IR rubber vulcanized with different cross-link agents

    Directory of Open Access Journals (Sweden)

    P. Posadas

    2016-01-01

    Full Text Available This study evaluates the formation of radical species in natural rubber (NR and poly-isoprene rubber (IR during the vulcanization process and the uniaxial deformation of the formed networks by means of Electron Spin Resonance (ESR. Vulcanization of NR and IR always shows a radical pathway, where the different vulcanization systems dictate the concentration of radical species in the course of this complex process. The greatest concentration of radicals were detected during the vulcanization with sulfur/accelerator based on efficient systems (EV, followed by conventional (CV and sulfur donor systems, whereas azide and organic peroxide agents showed smaller concentration of radicals. Independently of the vulcanization system, certain amount of radicals was detected on the vulcanized samples after the end of the vulcanization process. Comparison between different matrices demonstrates that NR always shows higher concentration of radicals than IR in the vulcanization process as well as during uniaxial deformation, fact that could be associated to the presence of nonrubber components in NR.

  14. Qualitative risk assessment for the 100-NR-2 Operable Unit. Revision 1

    International Nuclear Information System (INIS)

    1995-03-01

    This qualitative risk assessment provides information about the 100- NR-2 Groundwater Operable Unit of the Hanford reservation. Topics discussed in this report are: data evaluation; human health risk assessment overview; ecological evaluations; summary of uncertainty; results of both the ecological and human health evaluations; toxicity assessment; risk characterization; and a summary of contaminants of potential concern

  15. Schostakowitsch: Sinfonie Nr. 12 op. 112 (Das Jahr 1917) / Hans-Christian Dadelsen

    Index Scriptorium Estoniae

    Dadelsen, Hans-Christian

    1991-01-01

    Uuest heliplaadist "Schostakowitsch: Sinfonie Nr. 12 op. 112 (Das Jahr 1917). Hamlet-Suite op. 32a. Das goldene Zeitalter [The Age of Gold] (Suite) op. 22a. Göteborger Symphoniker, Neeme Järvi". DG CD 431 688-2 (WD:77'21") DDD

  16. Behavioral assays for evaluating host preferences of Euwallacea nr. fornicatus (Coleoptera: Curculionidae: Scolytinae).

    Science.gov (United States)

    In 2010, the exotic ambrosia beetle, Euwallacea nr. fornicatus Eichhoff (Coleoptera: Curculionidae: Scolytinae) was first discovered in Florida avocado groves. Introduction of its symbiotic Fusarium spp. fungi into galleries in the xylem tissue results in Fusarium-dieback disease. Unlike most ambros...

  17. Mahler: Sinfonie Nr. 8 Es-Dur (Sinfonie der Tausend) / Norbert Rüdell

    Index Scriptorium Estoniae

    Rüdell, Norbert

    1995-01-01

    Uuest heliplaadist "Mahler: Sinfonie Nr. 8 Es-Dur (Sinfonie der Tausend). Estonian Boys' Choir, Brunnsbo Children's Choir, Gothenburg Opera Chorus, Royal Stockholm Philharmonic Choir, Gothenburg Symphony Chorus, Gothenburg Opera Chorus, Gothenburg Symphony Orchestra, Neeme Järvi. BIS/Disco-Center CD 700 (WD:72'18")

  18. Barber: Sinfonie Nr. 1, Op. 9, Neeme Järvi / Bernhard Uske

    Index Scriptorium Estoniae

    Uske, Bernhard

    1991-01-01

    Uuest heliplaadist "Barber: Sinfonie Nr. 1, Op. 9. Ouvertüre School for Scandal, Op. 5; Beach: Sinfonie e-Moll, Op. 32, "Gaelic". Detroit Symphony Orchestra /Neeme Järvi". Chandes cassette ABTD 1550; CD CHAN 8958 (72 minutes)

  19. SRC Inhibition Reduces NR2B Surface Expression and Synaptic Plasticity in the Amygdala

    Science.gov (United States)

    Sinai, Laleh; Duffy, Steven; Roder, John C.

    2010-01-01

    The Src protein tyrosine kinase plays a central role in the regulation of N-methyl-d-aspartate receptor (NMDAR) activity by regulating NMDAR subunit 2B (NR2B) surface expression. In the amygdala, NMDA-dependent synaptic plasticity resulting from convergent somatosensory and auditory inputs contributes to emotional memory; however, the role of Src…

  20. Risk of influenza A(nrH7N9) pandemic in the eastern Mediterranean ...

    African Journals Online (AJOL)

    nrH7N9) infection in humans (1). Countries from the eastern Mediterranean region have high rates of viral hepatitis infection. The World Health. Organization (WHO) estimates that the HBsAg preva- lence in the eastern Mediterranean region ...

  1. Influence of acetone extract from natural rubber on the structure and interface interaction in NR/silica composites

    Science.gov (United States)

    Xu, Tiwen; Jia, Zhixin; Wu, Lianghui; Chen, Yongjun; Luo, Yuanfang; Jia, Demin; Peng, Zheng

    2017-11-01

    It is well known that the coupling reagents as the additional modifiers were often used to improve the reinforcement effect of silica filled natural rubber. Actually, the commercial raw NR is a mixture consisting of polyisoprene and non-isoprene, where the latter one might have impact on the properties of NR/silica composites as an inartificial modifier inside. Thus, investigating the effect of non-isoprene compounds on the structure and properties of NR/silica composites is a novel approach to disclose the peculiarity of NR, which is meaningful to the assessment of NR quality. In this paper, the influences of acetone extract (AE) from natural rubber on the structure and mechanical properties of NR/silica composites were studied. Then the interfacial interactions between AE and silica were also illustrated through Fourier transform infrared spectroscopy (FTIR), thermogravimetic analysis (TGA), X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). Results demonstrated the existence of hydrogen bond between silica and AE, also the covalent bond induced by esterification reaction between sbnd COOH and Sisbnd OH, which resulted in an increase of constrained regions around silica surface leading to the promotions on mechanical and dynamical properties of NR/silica composites significantly.

  2. A role of periaqueductal grey NR2B-containing NMDA receptor in mediating persistent inflammatory pain

    Directory of Open Access Journals (Sweden)

    Yang Qi

    2009-12-01

    Full Text Available Abstract The midbrain periaqueductal grey (PAG is a structure known for its roles in pain transmission and modulation. Noxious stimuli potentiate the glutamate synaptic transmission and enhance glutamate NMDA receptor expression in the PAG. However, little is known about roles of NMDA receptor subunits in the PAG in processing the persistent inflammatory pain. The present study was undertaken to investigate NR2A- and NR2B-containing NMDA receptors in the PAG and their modulation to the peripheral painful inflammation. Noxious stimuli induced by hind-paw injection of complete Freund's adjuvant (CFA caused up-regulation of NR2B-containing NMDA receptors in the PAG, while NR2A-containing NMDA receptors were not altered. Whole-cell patch-clamp recordings revealed that NMDA receptor mediated mEPSCs were increased significantly in the PAG synapse during the chronic phases of inflammatory pain in mice. PAG local infusion of Ro 25-6981, an NR2B antagonist, notably prolonged the paw withdrawal latency to thermal radian heat stimuli bilaterally in rats. Hyperoside (Hyp, one of the flavonoids compound isolated from Rhododendron ponticum L., significantly reversed up-regulation of NR2B-containing NMDA receptors in the PAG and exhibited analgesic activities against persistent inflammatory stimuli in mice. Our findings provide strong evidence that up-regulation of NR2B-containing NMDA receptors in the PAG involves in the modulation to the peripheral persistent inflammatory pain.

  3. Behavioral analysis of NR2C knockout mouse reveals deficit in acquisition of conditioned fear and working memory.

    Science.gov (United States)

    Hillman, Brandon G; Gupta, Subhash C; Stairs, Dustin J; Buonanno, Andres; Dravid, Shashank M

    2011-05-01

    N-methyl-D-aspartate (NMDA) receptors play an important role in excitatory neurotransmission and mediate synaptic plasticity associated with learning and memory. NMDA receptors are composed of two NR1 and two NR2 subunits and the identity of the NR2 subunit confers unique electrophysiologic and pharmacologic properties to the receptor. The precise role of NR2C-containing receptors in vivo is poorly understood. We have performed a battery of behavioral tests on NR2C knockout/nβ-galactosidase knock-in mice and found no difference in spontaneous activity, basal anxiety, forced-swim immobility, novel object recognition, pain sensitivity and reference memory in comparison to wildtype counterparts. However, NR2C knockout mice were found to exhibit deficits in fear acquisition and working memory compared to wildtype mice. Deficit in fear acquisition correlated with lack of fear conditioning-induced plasticity at the thalamo-amygdala synapse. These findings suggest a unique role of NR2C-containing receptors in associative and executive learning representing a novel therapeutic target for deficits in cognition. Copyright © 2011 Elsevier Inc. All rights reserved.

  4. HCV core protein promotes hepatocyte proliferation and chemoresistance by inhibiting NR4A1

    Energy Technology Data Exchange (ETDEWEB)

    Tan, Yongsheng, E-mail: yongshengtanwhu@126.com; Li, Yan, E-mail: liyansd2@163.com

    2015-10-23

    This study investigated the effect of HCV core protein on the proliferation of hepatocytes and hepatocellular carcinoma cells (HCC), the influence of HCV core protein on HCC apoptosis induced by the chemotherapeutic agent cisplatin, and the mechanism through which HCV core protein acts as a potential oncoprotein in HCV-related HCC by measuring the levels of NR4A1 and Runt-related transcription factor 3 (RUNX3), which are associated with tumor suppression and chemotherapy resistance. In the present study, PcDNA3.1-core and RUNX3 siRNA were transfected into LO2 and HepG2 cells using Lipofectamine 2000. LO2-core, HepG2-core, LO2-RUNX3 {sup low} and control cells were treated with different concentrations of cisplatin for 72 h, and cell proliferation and apoptosis were assayed using the CellTiter 96{sup ®}Aqueous Non-Radioactive Cell Proliferation Assay Kit. Western blot and real time PCR analyses were used to detect NR4A1, RUNX3, smad7, Cyclin D1 and BAX. Confocal microscopy was used to determine the levels of NR4A1 in HepG2 and HepG2-core cells. The growth rate of HepG2-core cells was considerably greater than that of HepG2 cells. HCV core protein increased the expression of cyclin D1 and decreased the expressions of NR4A1 and RUNX3. In LO2 – RUNX3 {sup low}, the rate of cell proliferation and the level of cisplatin resistance were the same as in the LO2 -core. These results suggest that HCV core protein decreases the sensitivity of hepatocytes to cisplatin by inhibiting the expression of NR4A1 and promoting the expression of smad7, which negatively regulates the TGF-β pathway. This effect results in down regulation of RUNX3, a target of the TGF-β pathway. Taken together, these findings indicate that in hepatocytes, HCV core protein increases drug resistance and inhibits cell apoptosis by inhibiting the expressions of NR4A1 and RUNX3. - Highlights: • HCV core protein inhibits HepG2 cell sensitivity to cisplatin. • Core expression in HepG2 decreases

  5. HCV core protein promotes hepatocyte proliferation and chemoresistance by inhibiting NR4A1

    International Nuclear Information System (INIS)

    Tan, Yongsheng; Li, Yan

    2015-01-01

    This study investigated the effect of HCV core protein on the proliferation of hepatocytes and hepatocellular carcinoma cells (HCC), the influence of HCV core protein on HCC apoptosis induced by the chemotherapeutic agent cisplatin, and the mechanism through which HCV core protein acts as a potential oncoprotein in HCV-related HCC by measuring the levels of NR4A1 and Runt-related transcription factor 3 (RUNX3), which are associated with tumor suppression and chemotherapy resistance. In the present study, PcDNA3.1-core and RUNX3 siRNA were transfected into LO2 and HepG2 cells using Lipofectamine 2000. LO2-core, HepG2-core, LO2-RUNX3 "l"o"w and control cells were treated with different concentrations of cisplatin for 72 h, and cell proliferation and apoptosis were assayed using the CellTiter 96"®Aqueous Non-Radioactive Cell Proliferation Assay Kit. Western blot and real time PCR analyses were used to detect NR4A1, RUNX3, smad7, Cyclin D1 and BAX. Confocal microscopy was used to determine the levels of NR4A1 in HepG2 and HepG2-core cells. The growth rate of HepG2-core cells was considerably greater than that of HepG2 cells. HCV core protein increased the expression of cyclin D1 and decreased the expressions of NR4A1 and RUNX3. In LO2 – RUNX3 "l"o"w, the rate of cell proliferation and the level of cisplatin resistance were the same as in the LO2 -core. These results suggest that HCV core protein decreases the sensitivity of hepatocytes to cisplatin by inhibiting the expression of NR4A1 and promoting the expression of smad7, which negatively regulates the TGF-β pathway. This effect results in down regulation of RUNX3, a target of the TGF-β pathway. Taken together, these findings indicate that in hepatocytes, HCV core protein increases drug resistance and inhibits cell apoptosis by inhibiting the expressions of NR4A1 and RUNX3. - Highlights: • HCV core protein inhibits HepG2 cell sensitivity to cisplatin. • Core expression in HepG2 decreases expression of NR4A1

  6. The orphan nuclear receptor NR4A1 (Nur77) regulates oxidative and endoplasmic reticulum stress in pancreatic cancer cells.

    Science.gov (United States)

    Lee, Syng-Ook; Jin, Un-Ho; Kang, Jeong Han; Kim, Sang Bae; Guthrie, Aaron S; Sreevalsan, Sandeep; Lee, Ju-Seog; Safe, Stephen

    2014-04-01

    NR4A1 (Nur77, TR3) is an orphan nuclear receptor that is overexpressed in pancreatic cancer and exhibits pro-oncogenic activity. RNA interference of NR4A1 expression in Panc-1 cells induced apoptosis and subsequent proteomic analysis revealed the induction of several markers of endoplasmic reticulum stress, including glucose-related protein 78 (GRP78), CCAAT/enhancer-binding protein-homologous protein (CHOP), and activating transcription factor-4 (ATF-4). Treatment of pancreatic cancer cells with the NR4A1 antagonist 1,1-bis(3'-indolyl)-1-(p-hydroxyphenyl)methane (DIM-C-pPhOH) gave similar results. Moreover, both NR4A1 knockdown and DIM-C-pPhOH induced reactive oxygen species (ROS), and induction of ROS and endoplasmic reticulum stress by these agents was attenuated after cotreatment with antioxidants. Manipulation of NR4A1 expression coupled with gene expression profiling identified a number of ROS metabolism transcripts regulated by NR4A1. Knockdown of one of these transcripts, thioredoxin domain containing 5 (TXNDC5), recapitulated the elevated ROS and endoplasmic reticulum stress; thus, demonstrating that NR4A1 regulates levels of endoplasmic reticulum stress and ROS in pancreatic cancer cells to facilitate cell proliferation and survival. Finally, inactivation of NR4A1 by knockdown or DIM-C-pPhOH decreased TXNDC5, resulting in activation of the ROS/endoplasmic reticulum stress and proapoptotic pathways. The NR4A1 receptor is pro-oncogenic, regulates the ROS/endoplasmic reticulum stress pathways, and inactivation of the receptor represents a novel pathway for inducing cell death in pancreatic cancer. Mol Cancer Res; 12(4); 527-38. ©2014 AACR.

  7. IGF-1-Involved Negative Feedback of NR2B NMDA Subunits Protects Cultured Hippocampal Neurons Against NMDA-Induced Excitotoxicity.

    Science.gov (United States)

    Li, Yun; Sun, Wei; Han, Song; Li, Jianing; Ding, Shu; Wang, Wei; Yin, Yanling

    2017-01-01

    Insulin-like growth factor 1 (IGF-1) is a multifunctional protein involved in neuronal polarity and axonal guidance. In our previous study, it was discovered that IGF-1 alleviated 50-μM NMDA-induced excitotoxicity against neuronal autophagy via depression of NR2B p-Ser1303 activation. However, it was found that NMDA at a higher dose did not cause neuronal autophagy. And, the performance of IGF-1 under severe excitotoxicity still needs to be clarified. In this study, we observed that IGF-1 can salvage the hippocampal neurons in an autophagy-independent manner after 150-μM NMDA exposure using thiazolyl blue tetrazolium bromide (MTT), lactate dehydrogenase (LDH), Western blot assay, and transmission electron microscopy. In addition, over-activation of post-synaptic NMDARs was found with the whole-cell patch clamp recording method. In order to explore whether there is a positive feedback way for post-synaptic NMDARs and the different pathway caused by 150 μM NMDA, the phosphorylation level of Fyn and the phosphorylation site of NR2B were investigated. It was observed that NR2B p-Tyr1472 was increased by the activation of Fyn after 150-μM NMDA exposure. When the neutralizing antibody against NR2B p-Ser1303 was added into the medium, both the activations of Fyn and NR2B p-Tyr1472 were blocked, suggesting NR2B p-Ser1303 may be the initial step of NMDA-induced excitotoxicity. In addition, since IGF-1 can block the initial step of NR2B activation, its effect is concluded to continue with the development of excitotoxicity. Overall, this study strongly indicates that the relationship between different phosphorylation sites of NR2B should be laid more emphasis on, which may be a vital target for the NR2B-involved excitotoxicity.

  8. PURIFICACIÓN DE IgY CONTRA LA SUBUNIDAD NR3 DEL RECEPTOR NMDA DE CEREBRO DE RATA

    Directory of Open Access Journals (Sweden)

    Gina Méndez C

    2008-04-01

    Full Text Available Objetivo. Obtener anticuerpos tipo IgY contra péptidos sintéticos de las subunidades NR3A y NR3B del receptor NMDA de ratas, para reconocer y seguir la expresión de estas subunidades en extractos de cerebro de rata de diferentes edades. Materiales y métodos. Se diseñaron dos péptidos empleando los sistemas de la base de datos Entrez y el programa ClustalW-PBIL de alineamientos múltiples contra las subunidades NR3A y NR3B del receptor NMDA; una vez sintetizados por el método SSPS-fmoc fueron utilizados para inocular gallinas (Gallus gallus, variedad Hy Line Brown de 16 semanas de edad; al cabo de 57 días postinoculación se purificó IgY específica y se enfrentaron a extractos de cerebro de rata postnatal y adulta. Resultados. Se detectaron las subunidades NR3A y NR3B y se relacionó su expresión con la edad del animal; siendo mayor la expresión de la subunidad NR3A en extracto de cerebro de rata postnatal. No se encontró diferencia marcada en la expresión de la subunidad NR3B en las edades mencionadas. Conclusiones. Esta es la primera investigación que emplea proteína nativa para el reconocimiento de la subunidad NR3 del receptor NMDA, lo cual muestra la especificidad de los anticuerpos generados y contribuye con el entendimiento de las funciones de este receptor y su relación con la regulación de la memoria espacial.

  9. Uso de una conantokina y anticuerpos policlonales para identificar la subunidad NR2B del receptor

    Directory of Open Access Journals (Sweden)

    Leonardo Lareo,†

    2010-12-01

    Full Text Available Objetivo. Proponer una metodología de identificación de la subunidad NR2B, mediante el uso de conantokina G, así como una adecuada extracción de la subunidad NR2B. Materiales y métodos. Se ensayaron dos metodologías para la extracción de la subunidad NR2B de cerebro de rata adulta, la primera buscó la extracción de la subunidad a partir de la membrana mediante la utilización del detergente deoxicolato de sodio y la segunda, garantizó primero la solubilización y eliminación de proteínas citoplasmáticas para luego realizar la extracción de la subunidad mediante el uso del mismo detergente, a partir del pellet generado en la centrifugación del extracto obtenido. Adicionalmente se biotiniló la conantokina G para evaluar su eficiencia en la identificación de la subunidad y comparar los resultados con los obtenidos por metodologías tradicionales como DOT-BLOT, WESTERN-BLOT, ELISA e Inmunohistoquímica. Resultados. La segunda metodología mostró mayor extracción de NR2B por lo que se seleccionó para la realización de los extractos posteriores. Los ensayos de identificación con la conantokina biotinilada evidenciaron interferencia en el reconocimiento, haciéndose necesaria la identificación de la presencia de la subunidad NR2B mediante el uso de anticuerpos policlonales en los ensayos mencionados. Conclusiones. Se propone que hay un impedimento de tipo estérico en el marcaje de la conantokina con la biotina lo que no favorece la interacción de este péptido con la subunidad.

  10. Zum Bildungspotenzial biblischer Texte

    Directory of Open Access Journals (Sweden)

    Theis, Joachim

    2017-11-01

    Full Text Available Biblical education as a holistic process goes far beyond biblical learning. It must be understood as a lifelong process, in which both biblical texts and their understanders operate appropriating their counterpart in a dialogical way. – Neither does the recipient’s horizon of understanding appear as an empty room, which had to be filled with the text only, nor is the latter a dead material one could only examine cognitively. The recipient discovers the meaning of the biblical text recomposing it by existential appropriation. So the text is brought to live in each individual reality. Both scientific insights and subjective structures as well as the understanders’ community must be included to avoid potential one-sidednesses. Unfortunately, a special negative association obscures the approach of the bible very often: Still biblical work as part of religious education appears in a cognitively oriented habit, which is neither regarding the vitality and sovereignty of the biblical texts nor the students’ desire for meaning. Moreover, the bible is getting misused for teaching moral terms or pontifications. Such downfalls can be disrupted by biblical didactics which are empowerment didactics. Regarding the sovereignty of biblical texts, these didactics assist the understander with his/her individuation by opening the texts with focus on the understander’s otherness. Thus each the text and the recipient become subjects in a dialogue. The approach of the Biblical-Enabling-Didactics leads the Bible to become always new a book of life. Understanding them from within their hermeneutics, empowerment didactics could be raised to the principle of biblical didactics in general and grow into an essential element of holistic education.

  11. Herkommer zum Hinschauen

    OpenAIRE

    Schilling, Jörg

    2011-01-01

    Tagung: Moderne Architektur exemplarisch. Hans Herkommer (1887-1956). Technische Universität Kaiserslautern, Lehrgebiet Geschichte und Theorie der Architektur. Prof. Dr. phil. Matthias Schirren. 28.-29. Oktober 2010

  12. Ein Experiment zum Anlegerverhalten

    OpenAIRE

    Weber, Martin; Camerer, Colin F.

    1992-01-01

    Portfolio selection is one important example of decision making under risk. We empirically investigate how decision makers behave when making these decisions, As a baseline we compare this intuitive decision making with the optimal decision making described by Markowitz' portfolio theory. Our results show that intuitive behavior is quite different from optimal behavior. Especially, subjects did not hold the optimal risky portfolio and they trade much more than predicted by portfol...

  13. Zum Ökologieschwerpunkt

    DEFF Research Database (Denmark)

    Czeskleba-Dupont, Rolf

    2009-01-01

    Sammenfaldet mellem en økologisk og en økonomisk krise blev allerede diskuteret i Das Argument 93/1975 med dets destruktive følger i forhold til nødvendige økologiske forbedringer. I dag diskuteres en 'Grøn New Deal' som løsning for kapitalismen og for klimaproblemet. Dette problematiseres ud fra...

  14. Zum Studium der Anthroposophie

    OpenAIRE

    Schlüter, Martin

    2014-01-01

    Johann Wolfgang v. Goethe und Rudolf Steiner haben beide auf verschiedenen Wegen eine Denkungsart entwickelt, deren Quelle diese Anschauungskraft ist. Eine Denkungsart, die nicht dabei stehenbleibt über die Dinge und Wesen nachzudenken, sondern die sie vielmehr in Gedankenform erstehen und so ihr Wesen unmittelbar erlebbar werden lässt. Goethe wandte sich dabei den Naturreichen zu und gestaltete seine ‚gegenständliche' Denkungsart zu einer naturwissenschaftlichen Methode aus, die er für weit ...

  15. LED; Zum Thema LED

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-07-01

    This collection of articles on the subject of light emitting diodes (LED) provides technical information on LED technology, examines latest developments and provides examples of LED use in practice. An 'ABC' of LED technology is presented and fifteen common LED mistakes are noted. The chances and risks of LED use are discussed as is the retrofitting of lighting installations with LEDs. The use of LEDs in street lighting is examined. The journal also includes interviews with architects and a lighting designer. Practical examples of the use of LEDs include the refurbished parliamentary library in Berne, their use in the bird sanctuary headquarters in Sempach, Switzerland, as well as LED use in sales outlets. Also, the use of LED lighting in a spa gazebo in Lucerne is examined.

  16. Vliv hipokampální aplikace Nr1/Nr2 antisense oligodeoxynukleotidů na expresi proteinů postsynaptické denzity a na prepulzní inhibici

    Czech Academy of Sciences Publication Activity Database

    Vrajová, M.; Klaschka, Jan; Tejkalová, H.; Bubeníková-Valešová, V.

    2011-01-01

    Roč. 15, Suppl. 2 (2011), s. 11-14 ISSN 1211-7579 R&D Projects: GA MŠk(CZ) 1M0517 Institutional research plan: CEZ:AV0Z10300504 Keywords : NMDA receptor * PSD proteins * antisense oligodeoxynucleotides for NMDA-NR1/NR2 subunits * prepulse inhibition Subject RIV: FL - Psychiatry, Sexuology http://www.tigis.cz/images/stories/psychiatrie/2011/s2/03_vrajova_cns_2-11.pdf

  17. Stalin Coctail: Schostakowitsch Kammersinfonie Nr. 2 (aus Streichquartett Nr. 3 op. 73). Pärt, Arvo: Collage über das Thema B-A-C-H / Susanne Benda

    Index Scriptorium Estoniae

    Benda, Susanne

    1995-01-01

    Uuest heliplaadist "Stalin Coctail: Schostakowitsch Kammersinfonie Nr. 2 (aus Streichquartett Nr. 3 op. 73). Pärt, Arvo: Collage über das Thema B-A-C-H. Cantus in memoriam B. Britten. Denisov Variationen über Haydns Kanon Tod ist ein langer Schlaf. Schtschedrin Stalin Coctail. Moskauer Virtuosen, Vladimir Spivakov. RCA/BMG-Ariola CD 09026 68061 2 (WD: 67'33")

  18. Reologi, sifat aging, termal, dan swelling dari campuran EPDM/NR dengan bahan pengisi carbon black N220

    Directory of Open Access Journals (Sweden)

    Arum Yuniari

    2016-06-01

    Full Text Available The blends of EPDM and NR are immiscible in nature and incompatible. Introducing maleic anhydride as a compatibilizer into the blends was expected to produce compatible blends with balanced mechanical properties. The purpose of this research was to determine the effect of EPDM/NR ratio on the rheological, aging, thermal and swelling properties of the blends. The variation of EPDM/NR ratio were 100/0, 80/20, 70/30, 60/40, 50/50, and 40/60 phr. The blends were mixed using two roll mill. The results showed that the addition of NR affects the maximum torque, scorch time, and optimum vulcanization time. In the blends, EPDM plays an important role in improving aging, swelling, thermal properties.

  19. Water Data Analysis 393937093090901 TURKEY CREEK NR SUMNER MO, FULBRIGHT RD NEAR SWAN LAKE NWR-2013-2016

    Data.gov (United States)

    Department of the Interior — WATER MONITORING STATION ANALYSIS – CALENDAR YEAR 2013 to 2016 SITE NUMBER: 393937093090901 SITE NAME: Turkey Creek nr Sumner MO, Fulbright Rd COOPERATION: Swan Lake...

  20. Wide spectrum of NR5A1‐related phenotypes in 46,XY and 46,XX individuals

    Science.gov (United States)

    Domenice, Sorahia; Machado, Aline Zamboni; Ferreira, Frederico Moraes; Ferraz‐de‐Souza, Bruno; Lerario, Antonio Marcondes; Lin, Lin; Nishi, Mirian Yumie; Gomes, Nathalia Lisboa; da Silva, Thatiana Evelin; Silva, Rosana Barbosa; Correa, Rafaela Vieira; Montenegro, Luciana Ribeiro; Narciso, Amanda; Costa, Elaine Maria Frade; Achermann, John C

    2016-01-01

    Steroidogenic factor 1 (NR5A1, SF‐1, Ad4BP) is a transcriptional regulator of genes involved in adrenal and gonadal development and function. Mutations in NR5A1 have been among the most frequently identified genetic causes of gonadal development disorders and are associated with a wide phenotypic spectrum. In 46,XY individuals, NR5A1‐related phenotypes may range from disorders of sex development (DSD) to oligo/azoospermia, and in 46,XX individuals, from 46,XX ovotesticular and testicular DSD to primary ovarian insufficiency (POI). The most common 46,XY phenotype is atypical or female external genitalia with clitoromegaly, palpable gonads, and absence of Müllerian derivatives. Notably, an undervirilized external genitalia is frequently seen at birth, while spontaneous virilization may occur later, at puberty. In 46,XX individuals, NR5A1 mutations are a rare genetic cause of POI, manifesting as primary or secondary amenorrhea, infertility, hypoestrogenism, and elevated gonadotropin levels. Mothers and sisters of 46,XY DSD patients carrying heterozygous NR5A1 mutations may develop POI, and therefore require appropriate counseling. Moreover, the recurrent heterozygous p.Arg92Trp NR5A1 mutation is associated with variable degrees of testis development in 46,XX patients. A clear genotype‐phenotype correlation is not seen in patients bearing NR5A1 mutations, suggesting that genetic modifiers, such as pathogenic variants in other testis/ovarian‐determining genes, may contribute to the phenotypic expression. Here, we review the published literature on NR5A1‐related disease, and discuss our findings at a single tertiary center in Brazil, including ten novel NR5A1 mutations identified in 46,XY DSD patients. The ever‐expanding phenotypic range associated with NR5A1 variants in XY and XX individuals confirms its pivotal role in reproductive biology, and should alert clinicians to the possibility of NR5A1 defects in a variety of phenotypes presenting with gonadal

  1. Activation of nuclear receptor NR5A2 increases Glut4 expression and glucose metabolism in muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Bolado-Carrancio, A. [Department of Molecular Biology, University of Cantabria, IDIVAL, Santander (Spain); Riancho, J.A. [Department of Internal Medicine, Hospital U.M. Valdecilla-IDIVAL, University of Cantabria, RETICEF, Santander (Spain); Sainz, J. [Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), CSIC-University of Cantabria, Santander (Spain); Rodríguez-Rey, J.C., E-mail: rodriguj@unican.es [Department of Molecular Biology, University of Cantabria, IDIVAL, Santander (Spain)

    2014-04-04

    Highlights: • NR5A2 expression in C2C12 is associated with myotube differentiation. • DLPC induces an increase in GLUT4 levels and glucose uptake in C2C12 myotubes. • In high glucose conditions the activation of NR5A2 inhibits fatty acids oxidation. - Abstract: NR5A2 is a nuclear receptor which regulates the expression of genes involved in cholesterol metabolism, pluripotency maintenance and cell differentiation. It has been recently shown that DLPC, a NR5A2 ligand, prevents liver steatosis and improves insulin sensitivity in mouse models of insulin resistance, an effect that has been associated with changes in glucose and fatty acids metabolism in liver. Because skeletal muscle is a major tissue in clearing glucose from blood, we studied the effect of the activation of NR5A2 on muscle metabolism by using cultures of C2C12, a mouse-derived cell line widely used as a model of skeletal muscle. Treatment of C2C12 with DLPC resulted in increased levels of expression of GLUT4 and also of several genes related to glycolysis and glycogen metabolism. These changes were accompanied by an increased glucose uptake. In addition, the activation of NR5A2 produced a reduction in the oxidation of fatty acids, an effect which disappeared in low-glucose conditions. Our results suggest that NR5A2, mostly by enhancing glucose uptake, switches muscle cells into a state of glucose preference. The increased use of glucose by muscle might constitute another mechanism by which NR5A2 improves blood glucose levels and restores insulin sensitivity.

  2. Orphan nuclear receptor NR4A1 is a negative regulator of DHT-induced rat preantral follicular growth.

    Science.gov (United States)

    Xue, Kai; Liu, Jia-yin; Murphy, Bruce D; Tsang, Benjamin K

    2012-12-01

    Nuclear receptor subfamily 4 group A member1 (NR4A1), an orphan nuclear receptor, is involved in the transcriptional regulation of thecal cell androgen biosynthesis and paracrine factor insulin-like 3 (INSL3) expression. Androgens are known to play an important regulatory role in ovarian follicle growth. Using a chronically androgenized rat model, a preantral follicle culture model and virus-mediated gene delivery, we examined the role and regulation of NR4A1 in the androgenic control of preantral follicular growth. In the present study, Ki67 staining was increased in preantral follicles on ovarian sections from 5α-dihydrotestosterone (DHT)-treated rats. Preantral follicles from DHT-treated rats cultured for 4 d exhibited increased growth and up-regulation of mRNA abundance of G(1)/S-specific cyclin-D2 (Ccnd2) and FSH receptor (Fshr). Similarly, DHT (1 μm) increased preantral follicular growth and Ccnd2 and Fshr mRNA abundance in vitro. The NR4A1 expression was high in theca cells and was down-regulated by DHT in vivo and in vitro. Forced expression of NR4A1 augmented preantral follicular growth, androstenedione production, and Insl3 expression in vitro. Inhibiting the action of androgen (with androgen receptor antagonist flutamide) or INSL3 (with INSL3 receptor antagonist INSL3 B-chain) reduced NR4A1-induced preantral follicular growth. Furthermore, NR4A1 overexpression enhanced DHT-induced preantral follicular growth, a response attenuated by inhibiting INSL3. In conclusion, DHT promotes preantral follicular growth and attenuates thecal NR4A1 expression in vivo and in vitro. Our findings are consistent with the notion that NR4A1 serves as an important point of negative feedback to minimize the excessive preantral follicle growth in hyperandrogenism.

  3. Activation of nuclear receptor NR5A2 increases Glut4 expression and glucose metabolism in muscle cells

    International Nuclear Information System (INIS)

    Bolado-Carrancio, A.; Riancho, J.A.; Sainz, J.; Rodríguez-Rey, J.C.

    2014-01-01

    Highlights: • NR5A2 expression in C2C12 is associated with myotube differentiation. • DLPC induces an increase in GLUT4 levels and glucose uptake in C2C12 myotubes. • In high glucose conditions the activation of NR5A2 inhibits fatty acids oxidation. - Abstract: NR5A2 is a nuclear receptor which regulates the expression of genes involved in cholesterol metabolism, pluripotency maintenance and cell differentiation. It has been recently shown that DLPC, a NR5A2 ligand, prevents liver steatosis and improves insulin sensitivity in mouse models of insulin resistance, an effect that has been associated with changes in glucose and fatty acids metabolism in liver. Because skeletal muscle is a major tissue in clearing glucose from blood, we studied the effect of the activation of NR5A2 on muscle metabolism by using cultures of C2C12, a mouse-derived cell line widely used as a model of skeletal muscle. Treatment of C2C12 with DLPC resulted in increased levels of expression of GLUT4 and also of several genes related to glycolysis and glycogen metabolism. These changes were accompanied by an increased glucose uptake. In addition, the activation of NR5A2 produced a reduction in the oxidation of fatty acids, an effect which disappeared in low-glucose conditions. Our results suggest that NR5A2, mostly by enhancing glucose uptake, switches muscle cells into a state of glucose preference. The increased use of glucose by muscle might constitute another mechanism by which NR5A2 improves blood glucose levels and restores insulin sensitivity

  4. Preliminary study of semi-refined carrageenan (SRC) as secondary gelling agent in natural rubber (NR) latex foam

    Science.gov (United States)

    Norhazariah, S.; Azura, A. R.; Azahari, B.; Sivakumar, R.

    2017-12-01

    Semi-refined carrageenan (SRC) product is considerably cheaper and easier to produce as a natural polysaccharide, which was utilized in food and other product application. However, the application in latex is limited. The aim of this work is to evaluate the SRC produced from low industrial grade seaweed (LIGS) in the latex foam application. The FTIR spectra showed the SRC produced as kappa type carrageenan with lower sulfur content compared to native LIGS. NR latex foam is produced by using the Dunlop method with some modifications. The effect of SRC loading as a secondary gelling agent in NR latex foam is investigated. The density and morphology of the NR latex foam with the addition of the SRC are analyzed. NR latex foam density increased with SRC loading and peaked at 1.8 phr SRC. The addition of SRC has induced the bigger cell size compared to the cell size of the control NR latex foam, as shown in the optical micrograph. It can be concluded that SRC LIGS could be acted as secondary gelling agent in NR latex foam.

  5. Biodegradation of NR Latex-based Materials via a Carbon Dioxide Evolution Method

    Directory of Open Access Journals (Sweden)

    F. M. S. Shabinah

    2017-12-01

    Full Text Available NR as a natural polymer has biodegradable characteristics and their existence was examined using CO2 evolution methods. The CO2 molecule produced by micro-organism metabolisms in the degradation system was quantified using a conventional acidimetric method. An aerobic system was determined as most suitable condition to be examined under this method. The presence of O2 in the system would help micro-organisms to destabilize the natural polymer. The material of LATZ, HA film and NR gloves showed significant weight loss and were able to produce CO2 evolution curves after 45 days in the biodegradation system compared to synthetic polyisoprene films. Gel permeation chromatography, fourier transform infrared spectroscopy and scanning electron micrograph were used to characterize the degraded sample at molecular and physical levels.

  6. Canonical realizations of the Lie algebra sp(2n,R)

    International Nuclear Information System (INIS)

    Havlicek, M.; Lassner, W.

    1975-01-01

    The generators of the Lie algebra of the symplectic group sp(2n,R) are, rezcurently, realied by means of polynomials in the quantum canonical variables qsub(i) and psub(i), i=1,...,d(2n-d);d=1,...,n. These realisations are skew-hermitean, the Casimir operators are realised by constant multiples of identity element and they depend on d free real parameters

  7. Long-term observations on the influence of groundwater level variations on BTEX concentrations in groundwater; Langzeituntersuchungen zum Einfluss von Grundwasserschwankungen auf die BTEX-Konzentration im Grundwasser

    Energy Technology Data Exchange (ETDEWEB)

    Puettmann, W. [J.W. Goethe-Universitaet Frankfurt a. M., Institut fuer Atmosphaere und Umwelt, AG Umweltanalytik, Frankfurt/Main (Germany); Hettwer, K.; Warrelmann, J. [Universitaet Bremen, Zentrum fuer Umweltforschung und Umwelttechnologie, Bremen (Germany); Gaab, S.

    2007-06-15

    A long-term study on natural attenuation and remediation in soil and groundwater at the former military base Schaeferhof-Sued (Niedersachsen) was performed at a former gasoline filling station. At this locality, a large residual source of benzene, toluene, ethylbenzene, xylenes (BTEX) and additional petroleum hydrocarbons is present in the soil. BTEX-concentrations in the groundwater and their correlation with groundwater level variations were monitored for three years. Within the monitoring period, a very dry summer was recorded, which caused the groundwater level to drop by 1.7 m and the BTEX concentrations to increase from 240 {mu}g/l to 1300 {mu}g/l at the site of contamination. The microbial degradation of BTEX was documented by data on consumption of electron acceptors (oxygen, nitrate or sulphate) and production of reduced products (Fe(II), methane). The degradation is further supported by the detection of metabolites. Therefore, the increasing BTEX concentrations were not a consequence of limited biological degradation. (orig.) [German] Auf dem frueher militaerisch genutzten Gelaende Schaeferhof-Sued (Niedersachsen) wurden im Bereich einer ehemaligen Abfuellstation fuer Kraftstoffe Langzeituntersuchungen zum natuerlichen Schadstoffabbau und -rueckhalt im Boden und Grundwasser durchgefuehrt. Der Standort weist eine hohe Restkontamination der Verbindungen Benzol, Toluol, Ethylbenzol und Xylole (BTEX), sowie Mineraloelkohlenwasserstoffen (MKW) in der ungesaettigten Bodenzone auf. Ueber einen Zeitraum von drei Jahren wurden die BTEX-Konzentrationen im Grundwasser und deren Abhaengigkeit von einer Aenderung des Grundwasserstandes untersucht und eine negative Korrelation der Schadstoffkonzentrationen mit der Hoehe des Grundwasserstandes festgestellt. Im Beobachtungszeitraum lag das sehr trockene Sommerhalbjahr 2003, was im Vergleich zum vorhergehenden Winterhalbjahr eine Absenkung des Grundwasserspiegels um 1,7 m zur Folge hatte und die BTEX-Konzentrationen am

  8. Genetic variation of the porcine NR5A1 is associated with meat color.

    Science.gov (United States)

    Görres, Andreas; Ponsuksili, Siriluck; Wimmers, Klaus; Muráni, Eduard

    2016-02-01

    Because of the central role of Steroidogenic factor 1 in the regulation of the development and function of steroidogenic tissues, including the adrenal gland, we chose the encoding gene NR5A1 as a candidate for stress response, meat quality and carcass composition in the domestic pig. To identify polymorphisms of the porcine NR5A1 we comparatively sequenced the coding, untranslated and regulatory regions in four commercial pig lines. Single nucleotide polymorphisms could be found in the 3' UTR and in an intronic enhancer, whereas no polymorphisms were detected in the proximal promoter and coding region. A subset of the detected polymorphisms was genotyped in Piétrain x (German Large White x German Landrace) and German Landrace pigs. For the same animals, carcass composition traits, meat quality characteristics and parameters of adrenal function were recorded. Associations with meat color were found for two of the discovered SNPs in Piétrain x (German Large White x German Landrace) and German Landrace pigs but no connections to parameters of adrenal function could be established. We conclude that NR5A1 variations influence meat color in a hypothalamus-pituitary-adrenal axis independent manner and that further regulatory regions need to be analyzed for genetic variations to understand the discovered effects.

  9. An open-label, non-randomized study of the pharmacokinetics of the nutritional supplement nicotinamide riboside (NR and its effects on blood NAD+ levels in healthy volunteers.

    Directory of Open Access Journals (Sweden)

    Sophia E Airhart

    Full Text Available The co-primary objectives of this study were to determine the human pharmacokinetics (PK of oral NR and the effect of NR on whole blood nicotinamide adenine dinucleotide (NAD+ levels.Though mitochondrial dysfunction plays a critical role in the development and progression of heart failure, no mitochondria-targeted therapies have been translated into clinical practice. Recent murine studies have reported associations between imbalances in the NADH/NAD+ ratio with mitochondrial dysfunction in multiple tissues, including myocardium. Moreover, an NAD+ precursor, nicotinamide mononucleotide, improved cardiac function, while another NAD+ precursor, nicotinamide riboside (NR, improved mitochondrial function in muscle, liver and brown adipose. Thus, PK studies of NR in humans is critical for future clinical trials.In this non-randomized, open-label PK study of 8 healthy volunteers, 250 mg NR was orally administered on Days 1 and 2, then uptitrated to peak dose of 1000 mg twice daily on Days 7 and 8. On the morning of Day 9, subjects completed a 24-hour PK study after receiving 1000 mg NR at t = 0. Whole-blood levels of NR, clinical blood chemistry, and NAD+ levels were analyzed.Oral NR was well tolerated with no adverse events. Significant increases comparing baseline to mean concentrations at steady state (Cave,ss were observed for both NR (p = 0.03 and NAD+ (p = 0.001; the latter increased by 100%. Absolute changes from baseline to Day 9 in NR and NAD+ levels correlated highly (R2 = 0.72, p = 0.008.Because NR increases circulating NAD+ in humans, NR may have potential as a therapy in patients with mitochondrial dysfunction due to genetic and/or acquired diseases.

  10. An open-label, non-randomized study of the pharmacokinetics of the nutritional supplement nicotinamide riboside (NR) and its effects on blood NAD+ levels in healthy volunteers.

    Science.gov (United States)

    Airhart, Sophia E; Shireman, Laura M; Risler, Linda J; Anderson, Gail D; Nagana Gowda, G A; Raftery, Daniel; Tian, Rong; Shen, Danny D; O'Brien, Kevin D

    2017-01-01

    The co-primary objectives of this study were to determine the human pharmacokinetics (PK) of oral NR and the effect of NR on whole blood nicotinamide adenine dinucleotide (NAD+) levels. Though mitochondrial dysfunction plays a critical role in the development and progression of heart failure, no mitochondria-targeted therapies have been translated into clinical practice. Recent murine studies have reported associations between imbalances in the NADH/NAD+ ratio with mitochondrial dysfunction in multiple tissues, including myocardium. Moreover, an NAD+ precursor, nicotinamide mononucleotide, improved cardiac function, while another NAD+ precursor, nicotinamide riboside (NR), improved mitochondrial function in muscle, liver and brown adipose. Thus, PK studies of NR in humans is critical for future clinical trials. In this non-randomized, open-label PK study of 8 healthy volunteers, 250 mg NR was orally administered on Days 1 and 2, then uptitrated to peak dose of 1000 mg twice daily on Days 7 and 8. On the morning of Day 9, subjects completed a 24-hour PK study after receiving 1000 mg NR at t = 0. Whole-blood levels of NR, clinical blood chemistry, and NAD+ levels were analyzed. Oral NR was well tolerated with no adverse events. Significant increases comparing baseline to mean concentrations at steady state (Cave,ss) were observed for both NR (p = 0.03) and NAD+ (p = 0.001); the latter increased by 100%. Absolute changes from baseline to Day 9 in NR and NAD+ levels correlated highly (R2 = 0.72, p = 0.008). Because NR increases circulating NAD+ in humans, NR may have potential as a therapy in patients with mitochondrial dysfunction due to genetic and/or acquired diseases.

  11. Optimalization activity of ZnO NR/TiO2 NR-P3HT as an active layer based on hybrid bulk heterojunction on dye sensitized solar cell (DSSC)

    International Nuclear Information System (INIS)

    Saputri, Liya Nikmatul Maula Zulfa; Ramelan, Ari Handono; Hanif, Qonita Awliya; Hasanah, Yesi Ihdina Fityatal; Prajanira, Lau Bekti; Wahyuningsih, Sayekti

    2016-01-01

    Dye sensitized solar cell (DSSC) with metal inorganic and conjugated organic polymer mixture, ZnO NR/TiO 2 NR-P3HT as an active layer based on hybrid bulk heterojunction has been studied. The hybrid material was used to optimize DSSC performs for better efficiency than only TiO 2 as an electrode. Synthesis of TiO 2 nanorods (NR) was conducted by ball milling 1000 rpm for 4 hours and strong base reaction by hydrothermal process at 120 °C overnight. And the ZnO NR was synthesized from Zn(NO 3 ) 2 .4H 2 O precusor by hydrotermal process at 90 °C for 5 hours and calcined on various temperature s of 400, 600, and 800 °C. ZnO NR was coated into an Tndium Tin Oxide (TTO) glass to collecting electron s effectively, where TiO 2 NR were incorporated with poly(3 -hexylthiophene) (P3HT) on various concentration s of 5, 10, 15 mg/mL to obtain a larger surface area. Material characterization were performed by X -Ray Diffraction (XRD) and Uv-Vis spectrophotometer. For an application of DSSC were measured by T-V Keithley Multimeter and the efficiency of DSSC at various P3HT’s concentrations of 5, 10, 15 mg/mL were 7.44 × 10 −3 , 0.0114, 0.0104, respectively. The maximum efficiency of DSSC was showed when TiO 2 NR-P3HT’s concentration was 10 mg/mL.

  12. Optimalization activity of ZnO NR/TiO2 NR-P3HT as an active layer based on hybrid bulk heterojunction on dye sensitized solar cell (DSSC)

    Energy Technology Data Exchange (ETDEWEB)

    Saputri, Liya Nikmatul Maula Zulfa; Ramelan, Ari Handono; Hanif, Qonita Awliya; Hasanah, Yesi Ihdina Fityatal; Prajanira, Lau Bekti; Wahyuningsih, Sayekti, E-mail: sayektiw@mipa.uns.ac.id [Chemistry Department, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Ir.Sutami 36A Kentingan Surakarta 57/26, Central Java (Indonesia)

    2016-04-19

    Dye sensitized solar cell (DSSC) with metal inorganic and conjugated organic polymer mixture, ZnO NR/TiO{sub 2} NR-P3HT as an active layer based on hybrid bulk heterojunction has been studied. The hybrid material was used to optimize DSSC performs for better efficiency than only TiO{sub 2} as an electrode. Synthesis of TiO{sub 2} nanorods (NR) was conducted by ball milling 1000 rpm for 4 hours and strong base reaction by hydrothermal process at 120 °C overnight. And the ZnO NR was synthesized from Zn(NO{sub 3}){sub 2}.4H{sub 2}O precusor by hydrotermal process at 90 °C for 5 hours and calcined on various temperature s of 400, 600, and 800 °C. ZnO NR was coated into an Tndium Tin Oxide (TTO) glass to collecting electron s effectively, where TiO{sup 2} NR were incorporated with poly(3 -hexylthiophene) (P3HT) on various concentration s of 5, 10, 15 mg/mL to obtain a larger surface area. Material characterization were performed by X -Ray Diffraction (XRD) and Uv-Vis spectrophotometer. For an application of DSSC were measured by T-V Keithley Multimeter and the efficiency of DSSC at various P3HT’s concentrations of 5, 10, 15 mg/mL were 7.44 × 10{sup −3}, 0.0114, 0.0104, respectively. The maximum efficiency of DSSC was showed when TiO{sup 2} NR-P3HT’s concentration was 10 mg/mL.

  13. Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone

    Energy Technology Data Exchange (ETDEWEB)

    Fukumasu, H.; Cordeiro, Y.G.; Rochetti, A.L.; Barra, C.N.; Sámora, T.S.; Strefezzi, R.F. [Laboratório de Oncologia Comparada e Translacional, Departmento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP (Brazil); Dagli, M.L.Z. [Laboratório de Oncologia Experimental e Comparada, Departmento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP (Brazil)

    2015-02-13

    Nuclear receptor subfamily 1, group I, member 3 (NR1I3) is reported to be a possible novel therapeutic target for some cancers, including lung, brain and hematopoietic tumors. Here, we characterized expression of NR1I3 in a mouse model of lung carcinogenesis induced by 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone (NNK), the most potent tobacco carcinogen. Lung tumors were collected from mice treated with NNK (400 mg/kg) and euthanized after 52 weeks. Benign and malignant lesions were formalin-fixed and paraffin-embedded for histology and immunohistochemistry, with samples snap-frozen for mRNA analysis. Immunohistochemically, we found that most macrophages and type I and II pneumocytes expressed NR1I3, whereas fibroblasts and endothelial cells were NR1I3{sup −}. Compared with benign lesions, malignant lesions had less NR1I3{sup +} tumor cells. Gene expression analysis also showed an inverse correlation between NR1I3 mRNA expression and tumor size (P=0.0061), suggesting that bigger tumors expressed less NR1I3 transcripts, in accordance with our immunohistochemical NR1I3 tests. Our results indicate that NR1I3 expression decreased during progression of malignant lung tumors induced by NNK in mice.

  14. nr0b1 (DAX1) mutation in zebrafish causes female-to-male sex reversal through abnormal gonadal proliferation and differentiation.

    Science.gov (United States)

    Chen, Sijie; Zhang, Hefei; Wang, Fenghua; Zhang, Wei; Peng, Gang

    2016-09-15

    Sex determinations are diverse in vertebrates. Although many sex-determining genes and pathways are conserved, the mechanistic roles of these genes and pathways in the genetic sex determination are not well understood. DAX1 (encoded by the NR0B1 gene) is a vertebrate specific orphan nuclear receptor that regulates gonadal development and sexual determination. In human, duplication of the NR0B1 gene leads to male-to-female sex reversal. In mice, Nr0b1 shows both pro-testis and anti-testis functions. We generated inheritable nr0b1 mutation in the zebrafish and found the nr0b1 mutation caused homozygous mutants to develop as fertile males due to female-to-male sex reversal. The nr0b1 mutation did not increase Caspase-3 labeling nor tp53 expression in the developing gonads. Introduction of a tp53 mutation into the nr0b1 mutant did not rescue the sex-reversal phenotype. Further examination revealed reduction in cell proliferation and abnormal somatic cell differentiation in the nr0b1 mutant gonads at the undifferentiated and bi-potential ovary stages. Together, our results suggest nr0b1 regulates somatic cell differentiation and cell proliferation to ensure normal sex development in the zebrafish. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  15. Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone

    International Nuclear Information System (INIS)

    Fukumasu, H.; Cordeiro, Y.G.; Rochetti, A.L.; Barra, C.N.; Sámora, T.S.; Strefezzi, R.F.; Dagli, M.L.Z.

    2015-01-01

    Nuclear receptor subfamily 1, group I, member 3 (NR1I3) is reported to be a possible novel therapeutic target for some cancers, including lung, brain and hematopoietic tumors. Here, we characterized expression of NR1I3 in a mouse model of lung carcinogenesis induced by 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone (NNK), the most potent tobacco carcinogen. Lung tumors were collected from mice treated with NNK (400 mg/kg) and euthanized after 52 weeks. Benign and malignant lesions were formalin-fixed and paraffin-embedded for histology and immunohistochemistry, with samples snap-frozen for mRNA analysis. Immunohistochemically, we found that most macrophages and type I and II pneumocytes expressed NR1I3, whereas fibroblasts and endothelial cells were NR1I3 − . Compared with benign lesions, malignant lesions had less NR1I3 + tumor cells. Gene expression analysis also showed an inverse correlation between NR1I3 mRNA expression and tumor size (P=0.0061), suggesting that bigger tumors expressed less NR1I3 transcripts, in accordance with our immunohistochemical NR1I3 tests. Our results indicate that NR1I3 expression decreased during progression of malignant lung tumors induced by NNK in mice

  16. Kalirin Binds the NR2B Subunit of the NMDA Receptor, Altering Its Synaptic Localization and Function

    KAUST Repository

    Kiraly, D. D.

    2011-08-31

    The ability of dendritic spines to change size and shape rapidly is critical in modulating synaptic strength; these morphological changes are dependent upon rearrangements of the actin cytoskeleton. Kalirin-7 (Kal7), a Rho guanine nucleotide exchange factor localized to the postsynaptic density (PSD), modulates dendritic spine morphology in vitro and in vivo. Kal7 activates Rac and interacts with several PSD proteins, including PSD-95, DISC-1, AF-6, and Arf6. Mice genetically lacking Kal7 (Kal7KO) exhibit deficient hippocampal long-term potentiation (LTP) as well as behavioral abnormalities in models of addiction and learning. Purified PSDs from Kal7KO mice contain diminished levels of NR2B, an NMDA receptor subunit that plays a critical role in LTP induction. Here we demonstrate that Kal7KO animals have decreased levels of NR2B-dependent NMDA receptor currents in cortical pyramidal neurons as well as a specific deficit in cell surface expression of NR2B. Additionally, we demonstrate that the genotypic differences in conditioned place preference and passive avoidance learning seen in Kal7KO mice are abrogated when animals are treated with an NR2B-specific antagonist during conditioning. Finally, we identify a stable interaction between the pleckstrin homology domain of Kal7 and the juxtamembrane region of NR2B preceding its cytosolic C-terminal domain. Binding of NR2B to a protein that modulates the actin cytoskeleton is important, as NMDA receptors require actin integrity for synaptic localization and function. These studies demonstrate a novel and functionally important interaction between the NR2B subunit of the NMDA receptor and Kalirin, proteins known to be essential for normal synaptic plasticity.

  17. A mollusk VDR/PXR/CAR-like (NR1J) nuclear receptor provides insight into ancient detoxification mechanisms

    Energy Technology Data Exchange (ETDEWEB)

    Cruzeiro, Catarina, E-mail: catarinarcruzeiro@hotmail.com [ICBAS - Institute of Biomedical Sciences Abel Salazar, U. Porto - University of Porto (Portugal); CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Lopes-Marques, Mónica, E-mail: monicaslm@hotmail.com [ICBAS - Institute of Biomedical Sciences Abel Salazar, U. Porto - University of Porto (Portugal); CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Ruivo, Raquel, E-mail: ruivo.raquel@gmail.com [CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Rodrigues-Oliveira, Nádia, E-mail: nadia.oliveira@ciimar.up.pt [CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Santos, Miguel M., E-mail: santos@ciimar.up.pt [CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); FCUP - Faculty of Sciences, Department of Biology, U. Porto (Portugal); Rocha, Maria João, E-mail: mjsrocha@netcabo.pt [ICBAS - Institute of Biomedical Sciences Abel Salazar, U. Porto - University of Porto (Portugal); CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Rocha, Eduardo, E-mail: erocha@icbas.up.pt [ICBAS - Institute of Biomedical Sciences Abel Salazar, U. Porto - University of Porto (Portugal); CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Castro, L. Filipe C., E-mail: filipe.castro@ciimar.up.pt [CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); FCUP - Faculty of Sciences, Department of Biology, U. Porto (Portugal)

    2016-05-15

    Highlights: • A nuclear receptor orthologue of the NR1J group is isolated from a mollusc. • The molluscan NR1J transactivates gene expression upon exposure to okadaic acid but not a pesticide, esfenvarelate and triclosan. • Lineage specific gene duplications and gene loss have occurred in the NR1J of protostomes with likely impacts on detoxification mechanisms. - Abstract: The origin and diversification of the metazoan endocrine systems represents a fundamental research issue in biology. Nuclear receptors are critical components of these systems. A particular group named VDR/PXR/CAR (NR1I/J) is central in the mediation of detoxification responses. While orthologues have been thoroughly characterized in vertebrates, a sparse representation is currently available for invertebrates. Here, we provide the first isolation and characterization of a lophotrochozoan protostome VDR/PXR/CAR nuclear receptor (NR1J), in the estuarine bivalve the peppery furrow shell (Scrobicularia plana). Using a reporter gene assay, we evaluated the xenobiotic receptor plasticity comparing the human PXR with the S. plana NR1Jβ. Our results show that the molluscan receptor responds to a natural toxin (okadaic acid) in a similar fashion to that reported for other invertebrates. In contrast, the pesticide esfenvalerate displayed a unique response, since it down regulated transactivation at higher concentrations, while for triclosan no response was observed. Additionally, we uncovered lineage specific gene duplications and gene loss in the gene group encoding NRs in protostomes with likely impacts on the complexity of detoxification mechanisms across different phyla. Our findings pave the way for the development of multi-specific sensor tools to screen xenobiotic compounds acting via the NR1I/J group.

  18. A mollusk VDR/PXR/CAR-like (NR1J) nuclear receptor provides insight into ancient detoxification mechanisms

    International Nuclear Information System (INIS)

    Cruzeiro, Catarina; Lopes-Marques, Mónica; Ruivo, Raquel; Rodrigues-Oliveira, Nádia; Santos, Miguel M.; Rocha, Maria João; Rocha, Eduardo; Castro, L. Filipe C.

    2016-01-01

    Highlights: • A nuclear receptor orthologue of the NR1J group is isolated from a mollusc. • The molluscan NR1J transactivates gene expression upon exposure to okadaic acid but not a pesticide, esfenvarelate and triclosan. • Lineage specific gene duplications and gene loss have occurred in the NR1J of protostomes with likely impacts on detoxification mechanisms. - Abstract: The origin and diversification of the metazoan endocrine systems represents a fundamental research issue in biology. Nuclear receptors are critical components of these systems. A particular group named VDR/PXR/CAR (NR1I/J) is central in the mediation of detoxification responses. While orthologues have been thoroughly characterized in vertebrates, a sparse representation is currently available for invertebrates. Here, we provide the first isolation and characterization of a lophotrochozoan protostome VDR/PXR/CAR nuclear receptor (NR1J), in the estuarine bivalve the peppery furrow shell (Scrobicularia plana). Using a reporter gene assay, we evaluated the xenobiotic receptor plasticity comparing the human PXR with the S. plana NR1Jβ. Our results show that the molluscan receptor responds to a natural toxin (okadaic acid) in a similar fashion to that reported for other invertebrates. In contrast, the pesticide esfenvalerate displayed a unique response, since it down regulated transactivation at higher concentrations, while for triclosan no response was observed. Additionally, we uncovered lineage specific gene duplications and gene loss in the gene group encoding NRs in protostomes with likely impacts on the complexity of detoxification mechanisms across different phyla. Our findings pave the way for the development of multi-specific sensor tools to screen xenobiotic compounds acting via the NR1I/J group.

  19. Kalirin Binds the NR2B Subunit of the NMDA Receptor, Altering Its Synaptic Localization and Function

    KAUST Repository

    Kiraly, D. D.; Lemtiri-Chlieh, Fouad; Levine, E. S.; Mains, R. E.; Eipper, B. A.

    2011-01-01

    The ability of dendritic spines to change size and shape rapidly is critical in modulating synaptic strength; these morphological changes are dependent upon rearrangements of the actin cytoskeleton. Kalirin-7 (Kal7), a Rho guanine nucleotide exchange factor localized to the postsynaptic density (PSD), modulates dendritic spine morphology in vitro and in vivo. Kal7 activates Rac and interacts with several PSD proteins, including PSD-95, DISC-1, AF-6, and Arf6. Mice genetically lacking Kal7 (Kal7KO) exhibit deficient hippocampal long-term potentiation (LTP) as well as behavioral abnormalities in models of addiction and learning. Purified PSDs from Kal7KO mice contain diminished levels of NR2B, an NMDA receptor subunit that plays a critical role in LTP induction. Here we demonstrate that Kal7KO animals have decreased levels of NR2B-dependent NMDA receptor currents in cortical pyramidal neurons as well as a specific deficit in cell surface expression of NR2B. Additionally, we demonstrate that the genotypic differences in conditioned place preference and passive avoidance learning seen in Kal7KO mice are abrogated when animals are treated with an NR2B-specific antagonist during conditioning. Finally, we identify a stable interaction between the pleckstrin homology domain of Kal7 and the juxtamembrane region of NR2B preceding its cytosolic C-terminal domain. Binding of NR2B to a protein that modulates the actin cytoskeleton is important, as NMDA receptors require actin integrity for synaptic localization and function. These studies demonstrate a novel and functionally important interaction between the NR2B subunit of the NMDA receptor and Kalirin, proteins known to be essential for normal synaptic plasticity.

  20. Keine Angst vor der Muttersprache – vor den (anderen Fremdsprachen aber auch nicht! Überlegungen zum Verhältnis von Einsprachigkeit und Zweisprachigkeit im Fremdsprachenunterricht

    Directory of Open Access Journals (Sweden)

    Frank G. Königs

    2015-10-01

    Full Text Available Die heute vorliegenden Forschungsergebnisse erlauben einen differenzierteren Blick auf die Rolle der Muttersprache bei der Aneignung einer fremden Sprache. Im vorliegenden Beitrag wird versucht, dieses differenzierte Bild nachzuzeichnen und die Rolle der Muttersprache aus unterschiedlichen Perspektiven auszuleuchten: Welche Erkenntnisse zur Sprachverarbeitung und zum Spracherwerb und welche vermittlungsmethodische Einsichten erlauben uns, die Rolle der Muttersprache zu bestimmen? Welche Bedeutung haben dabei der Bezug zur außerunterrichtlichen Realität der Lernenden und die dem scheinbar gegenüber-stehende natürliche Künstlichkeit des Fremdsprachenunterrichts? Was bedeutet dies alles für den Umgang mit Sprachen im fremdsprachlichen Klassenzimmer und im bilingualen Sachfachunterricht?  Recent research allows a more nuanced view of the role of the mother tongue in acquiring a foreign language. In this paper, an attempt is made to trace this differentiated image and illuminate the role of the mother tongue from different perspectives: What helps us to determine the role of the mother tongue given findings on language processing, language acquisition and (foreign language teaching methods? How do these results relate to the extracurricular realities of learners and the seemingly opposing natural artificiality of language teaching? What are the implications of this when dealing with languages in the foreign language classroom and CLIL?

  1. Investigation of the warm prestress effect by X-ray and microfractographic measurements; Roentgenografische und mikrofraktografische Untersuchungen zum Warmvorbeanspruchungs (WPS) - Effekt

    Energy Technology Data Exchange (ETDEWEB)

    Blumenauer, H.; Eichler, B.; Krempe, M.; Ude, J. [Magdeburg Univ. (Germany). Inst. fuer Werkstofftechnik und Werkstoffpruefung

    1998-11-01

    The work reported was to investigate the changes caused at the crack tip of a specimen with incipient crack and to assess their effects with regard to component fracture. The steels selected for testing are the pressure vessel steels 10MnMoNi5-5 and 17MoV8-4, and the experiments were made with CT-25 specimens. The conclusion drawn from the results obtained is that the WPS effect is due to a stronger energy dissipation in the prestressed area ahead of the crack tip, assisted by intrinsic stress-induced crack closing. (orig./CB) [Deutsch] Das Ziel dieser Arbeit ist es, die durch eine Vorbeanspruchung angerissener Proben hervorgerufenen Veraenderungen an der Rissspitze und ihre Auswirkungen auf das Bruchverhalten zu untersuchen. Die Untersuchungen wurden an den Druckbehaelterstaehlen 10MnMoNi5-5 und 17MoV8-4 durchgefuehrt. Die Versuche wurden an CT-25-Proben durchgefuehrt. Die Autoren kommen zum Schluss, dass der WPS-Effekt mit einer staerkeren Energiedissipation in der vorgeschaedigten Prozesszone vor der Rissspitze, unterstuetzt durch eigenspannungsbedingtes Rissschliessen, erklaert werden kann. (orig./MM)

  2. Investigations into the use of bacteria for enhanced recovery of petroleum; Untersuchungen zum Einsatz von Bakterien zur Erhoehung des Entoelungsgrades in Erdoellagerstaetten

    Energy Technology Data Exchange (ETDEWEB)

    Amro, M.

    1995-06-01

    Strains of bacteria were investigated with a view to their applicability and efficiency in enhanced recovery of petroleum. The mechanisms of enhanced recovery with bacteria were to be clarified as well. The experiments were carried out as static autoclave experiments without bedrock and as dynamic flooding experiments in a model pore space (Bentheim sandstone and carbonate rock) under the conditions of North German petroleum deposits. The static experiments carried out preliminary to the more time-consuming dynamic flooding experiments were to help with an optimized design of the injection experiments. (orig.) [Deutsch] Die vorliegende Arbeit hat zum Ziel, entoelungswirksame Bakterienstaemme zu untersuchen und hinsichtlich der durch sie bewirkten Erhoehung des Entoelungsgrades zu bewerten. Gleichzeitig sollen die Mechanismen aufgeklaert werden, die der Entoelungsverbesserung durch Einsatz von Bakterien zugrunde liegen. Die Experimente wurden als statische Autoklavenversuche ohne Lagerstaettengestein sowie als dynamische Flutversuche im Modellporenraum (Bentheimer Sandstein und karbonatisches Gesteinsmaterial) unter Bedingungen norddeutscher Lagerstaetten durchgefuehrt. Die statischen Versuche, die als Auswahltests den aufwendigeren dynamischen Flutversuchen vorgeschaltet waren, sollten dazu beitragen, die Flutversuche gezielt konzipieren zu koennen. (orig./EF)

  3. INTRAHIPPOCAMPAL ADMINISTRATION OF IBOTENIC ACID INDUCED CHOLINERGIC DYSFUNCTION via NR2A/NR2B EXPRESSION: IMPLICATIONS OF RESVERATROL AGAINST ALZHEIMER DISEASE PATHOPHYSIOLOGY

    Directory of Open Access Journals (Sweden)

    Chennakesavan eKarthick

    2016-04-01

    Full Text Available Although several drugs revealed moderate amelioration of symptoms, none of them have sufficient potency to prevent or reverse the progression towards Alzheimer’s disease (AD pathology. Resveratrol (RSV, a polyphenolic compound has shown an outstanding therapeutic effect on a broad spectrum of diseases like age-associated neurodegeneration, inflammation etc. The present study was thus conducted to assess the therapeutic efficacy of RSV in ameliorating the deleterious effects of Ibotenic acid (IBO in male Wistar rats. Stereotactic intrahippocampal administration of IBO (5µg/µl lesioned rats impairs cholinergic transmission, learning and memory performance that is rather related to AD and thus chosen as a suitable model to understand the drug efficacy in preventing AD pathophysiology. Since IBO is an agonist of glutamate, it is expected to exhibit an excitotoxic effect by altering glutamatergic receptors like NMDA receptor. The current study displayed significant alterations in the mRNA expression of NR2A and NR2B subunits of NMDA receptors, and further it is surprising to note that cholinergic receptors decreased in expression particularly α7-nAChR with increased m1AChR. RSV administration (20mg/kg body weight, i.p significantly reduced these changes in IBO induced rats. Glutamatergic and cholinergic receptor alterations were associated with significant changes in the behavioral parameters of rats induced by IBO. While RSV improved spatial learning performance, attenuated immobility and improvised open field activity in IBO induced rats. NR2B activation in the present study might mediate cell death through oxidative stress that form the basis of abnormal behavioral pattern in IBO induced rats. Interestingly, RSV that could efficiently encounter oxidative stress have significantly decreased stress markers viz., nitrite, PCO, and MDA levels by enhancing antioxidant status. Histopathological analysis displayed significant reduction in the

  4. Mice with subtle reduction of NMDA NR1 receptor subunit expression have a selective decrease in mismatch negativity: Implications for schizophrenia prodromal population.

    Science.gov (United States)

    Featherstone, Robert E; Shin, Rick; Kogan, Jeffrey H; Liang, Yuling; Matsumoto, Mitsuyuki; Siegel, Steven J

    2015-01-01

    Reductions in glutamate function are regarded as an important contributory factor in schizophrenia. However, there is a paucity of animal models characterized by developmental and sustained reductions in glutamate function. Pharmacological models using NMDA antagonists have been widely used but these typically produce only transient changes in behavior and brain function. Likewise, mice with homozygous constitutive reductions in glutamate receptor expression show stable brain and behavioral changes, but many of these phenotypes are more severe than the human disease. The current study examines a variety of schizophrenia-related EEG measures in mice with a heterozygous alteration of the NMDA receptor NR1 subunit gene (NR1) that is known to result in reduced NR1 receptor expression in the homozygous mouse (NR1-/-). (NR1+/-) mice showed a 30% reduction in NR1 receptor expression and were reared after weaning in either group or isolated conditions. Outcome measures include the response to paired white noise stimuli, escalating inter-stimulus intervals (ISIs) and deviance-related mismatch negativity (MMN). In contrast to what has been reported in (NR1-/-) mice and mice treated with NMDA antagonists, (NR1+/-) mice showed no change on obligatory Event Related Potential (ERP) measures including the murine P50 and N100 equivalents (P20 and N40), or measures of baseline or evoked gamma power. Alternatively, (NR1+/-) mice showed a marked reduction in response to a deviant auditory tone during MMN task. Data suggest that EEG response to deviant, rather than static, stimuli may be more sensitive for detecting subtle changes in glutamate function. Deficits in these heterozygous NR1 knockdown mice are consistent with data demonstrating MMN deficits among family members of schizophrenia patients and among prodromal patients. Therefore, the current study suggests that (NR1+/-) mice may be among the most sensitive models for increased vulnerability to schizophrenia. Copyright

  5. Degradation of blending vulcanized natural rubber and nitril rubber (NR/NBR) by dimethyl ether through variation of elastomer ratio

    Science.gov (United States)

    Saputra, A. H.; Juneva, S.; Sari, T. I.; Cifriadi, A.

    2018-04-01

    Dimethyl ether can cause degradation of the rubber material seal in some applications. In order to use of natural rubber in industry, research about a blending of natural rubber (NR) and nitrile rubber (NBR) to produce rubber to meet the standard seal material application were conducted. This study will observe the degradation mechanisms that occur in the blending natural rubber and nitrile rubber (NR/NBR) by dimethyl ether. Nitrile rubber types used in this study is medium quality nitrile rubber with 33% of acrylonitrile content (NBR33). The observed parameters are percent change in mass, mechanical properties and surface morphology. This study is limited to see the effect of variation vulcanized blending ratio (NR/NBR33) against to swelling. The increase of nitrile rubber (NBR33) ratio of blending rubber vulcanized can reduce the tensile strength and elongation. The best elastomer variation was obtained after comparing with the standard feasibility material of seal is rubber vulcanized blending (NR/NBR33) with ratio 40:60 NR: NBR.

  6. Spectroscopic studies on the interaction and sonodynamic damage of neutral red (NR) to bovine serum albumin (BSA)

    Energy Technology Data Exchange (ETDEWEB)

    Liu Bin; Guo Ying [Department of Pharmacy, Liaoning University, Shenyang 110036 (China); Wang, Jun, E-mail: wangjun890@126.co [Department of Chemistry, Liaoning University, Shenyang 110036 (China); Xu Rui [Department of Chemistry, Liaoning University, Shenyang 110036 (China); Wang Xin; Wang Dan; Zhang Liqun [Department of Pharmacy, Liaoning University, Shenyang 110036 (China); Xu Yongnan [Department of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016 (China)

    2010-06-15

    In this paper, the interaction of neutral red (NR) with bovine serum albumin (BSA) and the sonodynamic damage to BSA under ultrasonic irradiation was studied by means of ultraviolet-visible (UV-vis) and fluorescence spectra. The quenching constant (K{sub SV}=5.749x10{sup 4} L/mol), binding constant (K{sub A}=3.19x10{sup 4} L/mol) and binding site number (n=0.9462) were measured. The binding distance (r=2.47 nm) between NR and BSA was obtained according to Foester's non-radiative energy transfer theory. The damage process of BSA molecules was detected by the hyperchromic effect of UV-vis spectra and quenching of intrinsic fluorescence spectra. In addition, the influencing factors such as ultrasonic irradiation time and NR concentration on the damage to BSA molecules were also considered. The results showed that the damage degree is enhanced with the increase of ultrasonic irradiation time and NR concentration. The possible mechanism of sonodynamic damage to BSA molecules was mainly mediated by singlet oxygen ({sup 1}O{sub 2}). Otherwise, the binding and damaging sites to BSA molecules were also estimated by synchronous fluorescence. The results indicated that the NR is more vicinal to tryptophan (Trp) residue than to tyrosine (Tyr) residue and the damage site is also mainly at Trp residues. The research result will bring a certain significance to use sonosensitive drugs in the fields of tumor treatment.

  7. Review: Mäder, Maya (2017: Selbsterfahrung in der Psychotherapie (Self experience in Psychotherapy, Die Bedeutung für den Kompetenzerwerb in der Aus- und Weiterbildung zum transaktionalytischen Psychotherapeuten (The significance for getting competence in training of transactional psychotherapy Münster: Waxmann

    Directory of Open Access Journals (Sweden)

    Günther Mohr

    2017-07-01

    Full Text Available Review: Mäder, Maya (2017: Selbsterfahrung in der Psychotherapie (Self experience in Psychotherapy, Die Bedeutung für den Kompetenzerwerb in der Aus- und Weiterbildung zum transaktionalytischen Psychotherapeuten (The significance for getting competence in training of transactional psychotherapy Münster: Waxmann   Citation - APA format: Mohr, G. (2017. Review: Mäder, Maya (2017: Selbsterfahrung in der Psychotherapie (Self experience in Psychotherapy, Die Bedeutung für den Kompetenzerwerb in der Aus- und Weiterbildung zum transaktionalytischen Psychotherapeuten (The significance for getting competence in training of transactional psychotherapy Münster: Waxmann. International Journal of Transactional Analysis Research & Practice, 8(2, 49-50.

  8. Proposal for inclusion of the risk based inspection technique in Regulatory Standard NR 13; Proposta de inclusao da tecnica de inspecao baseada em risco na Norma Regulamentadora NR 13

    Energy Technology Data Exchange (ETDEWEB)

    Esteves, Vinicius Teixeira; Lima, Marco Aurelio Oliveira [Det Norske Veritas Ltda. (DNV), Rio de Janeiro, RJ (Brazil)

    2012-07-01

    In Brazil, the Regulatory Standard n. 13 (NR 13) establishes requirements for the inspection of boilers and pressure vessels which has main objective of preventing accidents with these types of equipment. Additionally, it has the Risk-Based Inspection (RBI) technique as an effective way to manage the mechanical integrity of various types of static mechanical equipment by through an inspection planning based on the risk factor. In this study, it is being proposed to include the RBI technique, in the NR 13, for the planning and definition of periods for the safety inspection of boilers and pressure vessels in order to promote an increase in the operational safety in process industries in Brazil. In this study it was carried out a critical analysis of NR 13 and RBI, and beyond that a bibliographic research of various international documents that relate the operational safety of pressurized equipment with the inspection activity, and the acceptability of RBI by governments, agencies and organizations around the world. It is considered that the inclusion and formal acceptance of RBI technique in the NR 13 must be accompanied by a rigorous control to avoid the 'trivialization' of its use and ensure the implementation rational, efficient and reliable. Finally, it was developed and suggested basic elements and minimum requirements to be inserted in the NR 13, to be attended, in order mandatory, by the companies that choose the implementation and use of the RBI technique as a tool for the planning of safety inspection of boilers and pressure vessels. It is concluded that the formal acceptance of the RBI technique in the NR 13 could aggregate much value to this standard, with regard to the prevention of accidents involving boilers or pressure vessels, and provide a technological jump to the companies that make use of RBI technique in Brazil. (author)

  9. Avaliação do cumprimento da NR-18 em função do porte de obra residencial e proposta de lista de verificação da NR-18

    OpenAIRE

    Costella, Marcelo Fabiano; Junges, Franciele Cristina; Pilz, Silvio Edmundo

    2014-01-01

    O presente trabalho investiga as reais condições dos ambientes de trabalho da indústria da construção, tendo em vista os problemas relacionados à segurança do trabalho nos canteiros de obras. Formulou-se a hipótese de haver discrepância no grau de cumprimento da NR-18 em obras de diferentes portes (pequena, média e grande). Baseado em uma lista de verificação do grau de cumprimento dos requisitos da NR-18, foram avaliados 115 canteiros de obra na cidade de Chapecó - SC. Para cada item da list...

  10. Origin of phase shift in atomic force microscopic investigation of the surface morphology of NR/NBR blend film

    Energy Technology Data Exchange (ETDEWEB)

    Thanawan, S. [Institute of Science and Technology for Research and Development, Mahidol University, Salaya, Nakhon Pathom 73170 (Thailand)], E-mail: ststw@mahidol.ac.th; Radabutra, S.; Thamasirianunt, P.; Amornsakchai, T.; Suchiva, K. [Department of Chemistry, Faculty of Science, Mahidol University, Salaya, Nakhon Pathom 73170 (Thailand)

    2009-01-15

    Atomic force microscopy (AFM) was used to study the morphology and surface properties of NR/NBR blend. Blends at 1/3, 1/1 and 3/1 weight ratios were prepared in benzene and formed film by casting. AFM phase images of these blends in tapping mode displayed islands in the sea morphology or matrix-dispersed structures. For blend 1/3, NR formed dispersed phase while in blends 1/1 and 3/1 phase inversion was observed. NR showed higher phase shift angle in AFM phase imaging for all blends. This circumstance was governed by adhesion energy hysteresis between the device tip and the rubber surface rather than surface stiffness of the materials, as proved by force distance measurements in the AFM contact mode.

  11. Origin of phase shift in atomic force microscopic investigation of the surface morphology of NR/NBR blend film.

    Science.gov (United States)

    Thanawan, S; Radabutra, S; Thamasirianunt, P; Amornsakchai, T; Suchiva, K

    2009-01-01

    Atomic force microscopy (AFM) was used to study the morphology and surface properties of NR/NBR blend. Blends at 1/3, 1/1 and 3/1 weight ratios were prepared in benzene and formed film by casting. AFM phase images of these blends in tapping mode displayed islands in the sea morphology or matrix-dispersed structures. For blend 1/3, NR formed dispersed phase while in blends 1/1 and 3/1 phase inversion was observed. NR showed higher phase shift angle in AFM phase imaging for all blends. This circumstance was governed by adhesion energy hysteresis between the device tip and the rubber surface rather than surface stiffness of the materials, as proved by force distance measurements in the AFM contact mode.

  12. Influence of different curing systems on the physico-mechanical properties and stability of SBR and NR rubbers

    Energy Technology Data Exchange (ETDEWEB)

    Basfar, A.A. E-mail: abasfar@kacst.edu.sa; Abdel-Aziz, M.M.; Mofti, S

    2002-01-01

    The physical properties of radiation, sulfur and peroxide-cured styrene-butadiene rubber (SBR) and natural rubber (NR) were compared. The dependence of the mechanical properties of the radiation-vulcanized SBR and NR on the coagent concentration and radiation dose was studied. The effect of thermal aging on the mechanical properties of the different rubber formulations was discussed. The radiation-cured formulations of SBR have superior mechanical properties and thermal stability compared with those of the chemically vulcanized compounds. Whereas, the radiation-cured formulations of NR have similar mechanical properties but superior thermal stability (based on the % change in E after thermal aging), when compared with those of the sulfur-vulcanized compounds and slightly better than those of the peroxide-vulcanized compounds.

  13. E-business solutions for utilities, - current state of implementation on the Internet; E-Business-Branchenloesungen - Untersuchung zum Implementierunsstand im Internet. Der Online-Weg zu den Privatkunden

    Energy Technology Data Exchange (ETDEWEB)

    Koch, M.; Baier, D. [Brandenburgische Technische Univ. Cottbus (Germany). Lehrstuhl fuer Marketing und Innovationsmanagement

    2003-04-22

    In the first part of the contribution the benefit of an e-business system in connection with internet was illustrated by an example. The current state of implementation is the subject of this second part. In an empirical study the websites of 600 utilities were analyzed in detail. The results of this analysis are described in the following. (orig.) [German] In [1] wird der Nutzen eines E-Business-Systems in Verbindung mit dem Internet an einem praktischen Beispiel veranschaulicht. Im Folgenden wird ein Ueberblick zum aktuellen Implementierungsstand in den Enegieversorgungsunternehmen gegeben. In einer empirischen Untersuchung wurden die Webseiten von 600 EVU analysiert, die Ergebnisse der Auswertung werden vorgestellt. (orig.)

  14. The Evolution of NR TrA (Nova TrA 2008) from 2008 through 2017

    Science.gov (United States)

    Walter, Frederick M.; Burwitz, Vadim; Kafka, Stella

    2018-06-01

    The classical nova NR TrA was discovered as an O-type optically-thick classical nova. There is no evidence that it formed dust. Within four years the envelope became sufficiently thin to reveal an eclipsing accretion disk-dominated system with orbitally-modulated permitted lines of C IV, N V, and O VI. XMM observations reveal a non-eclipsing soft X-ray source and a deeply-eclipsing UV continuum. We will present the first ten years of optical spectral evolution of this system accompanied by ten years of BVRIJHK photometry, with an eye to deciphering the current nature of the system.

  15. Investigation of associations between NR1D1, RORA and RORB genes and bipolar disorder.

    Directory of Open Access Journals (Sweden)

    Yin-Chieh Lai

    Full Text Available Several genes that are involved in the regulation of circadian rhythms are implicated in the susceptibility to bipolar disorder (BD. The current study aimed to investigate the relationships between genetic variants in NR1D1 RORA, and RORB genes and BD in the Han Chinese population. We conducted a case-control genetic association study with two samples of BD patients and healthy controls. Sample I consisted of 280 BD patients and 200 controls. Sample II consisted of 448 BD patients and 1770 healthy controls. 27 single nucleotide polymorphisms in the NR1D1, RORA, and RORB genes were genotyped using GoldenGate VeraCode assays in sample I, and 492 markers in the three genes were genotyped using Affymetrix Genome-Wide CHB Array in sample II. Single marker and gene-based association analyses were performed using PLINK. A combined p-value for the joining effects of all markers within a gene was calculated using the rank truncated product method. Multifactor dimensionality reduction (MDR method was also applied to test gene-gene interactions in sample I. All markers were in Hardy-Weinberg equilibrium (P>0.001. In sample I, the associations with BD were observed for rs4774388 in RORA (OR = 1.53, empirical p-value, P = 0.024, and rs1327836 in RORB (OR = 1.75, P = 0.003. In Sample II, there were 45 SNPs showed associations with BD, and the most significant marker in RORA was rs11639084 (OR = 0.69, P = 0.002, and in RORB was rs17611535 (OR = 3.15, P = 0.027. A combined p-value of 1.6×10-6, 0.7, and 1.0 was obtained for RORA, RORB and NR1D1, respectively, indicting a strong association for RORA with the risk of developing BD. A four way interaction was found among markers in NR1D1, RORA, and RORB with the testing accuracy 53.25% and a cross-validation consistency of 8 out of 10. In sample II, 45 markers had empirical p-values less than 0.05. The most significant markers in RORA and RORB genes were rs11639084 (OR = 0.69, P = 0.002, and rs17611535 (OR = 3

  16. Pacific Northwest National Laboratory Apatite Investigation at the 100-NR-2 Quality Assurance Project Plan

    Energy Technology Data Exchange (ETDEWEB)

    Fix, N. J.

    2008-03-28

    This Quality Assurance Project Plan provides the quality assurance requirements and processes that will be followed by staff working on the 100-NR-2 Apatite Project. The U.S. Department of Energy, Fluor Hanford, Inc., Pacific Northwest National Laboratory, and the Washington Department of Ecology agreed that the long-term strategy for groundwater remediation at 100-N would include apatite sequestration as the primary treatment, followed by a secondary treatment. The scope of this project covers the technical support needed before, during, and after treatment of the targeted subsurface environment using a new high-concentration formulation.

  17. NR2B Expression in Rat DRG Is Differentially Regulated Following Peripheral Nerve Injuries That Lead to Transient or Sustained Stimuli-Evoked Hypersensitivity.

    Science.gov (United States)

    Norcini, Monica; Sideris, Alexandra; Adler, Samantha M; Hernandez, Lourdes A M; Zhang, Jin; Blanck, Thomas J J; Recio-Pinto, Esperanza

    2016-01-01

    Following injury, primary sensory neurons undergo changes that drive central sensitization and contribute to the maintenance of persistent hypersensitivity. NR2B expression in the dorsal root ganglia (DRG) has not been previously examined in neuropathic pain models. Here, we investigated if changes in NR2B expression within the DRG are associated with hypersensitivities that result from peripheral nerve injuries. This was done by comparing the NR2B expression in the DRG derived from two modalities of the spared nerve injury (SNI) model, since each variant produces different neuropathic pain phenotypes. Using the electronic von Frey to stimulate the spared and non-spared regions of the hindpaws, we demonstrated that sural-SNI animals develop sustained neuropathic pain in both regions while the tibial-SNI animals recover. NR2B expression was measured at Day 23 and Day 86 post-injury. At Day 23 and 86 post-injury, sural-SNI animals display strong hypersensitivity, whereas tibial-SNI animals display 50 and 100% recovery from post-injury-induced hypersensitivity, respectively. In tibial-SNI at Day 86, but not at Day 23 the perinuclear region of the neuronal somata displayed an increase in NR2B protein. This retention of NR2B protein within the perinuclear region, which will render them non-functional, correlates with the recovery observed in tibial-SNI. In sural-SNI at Day 86, DRG displayed an increase in NR2B mRNA which correlates with the development of sustained hypersensitivity in this model. The increase in NR2B mRNA was not associated with an increase in NR2B protein within the neuronal somata. The latter may result from a decrease in kinesin Kif17, since Kif17 mediates NR2B transport to the soma's plasma membrane. In both SNIs, microglia/macrophages showed a transient increase in NR2B protein detected at Day 23 but not at Day 86, which correlates with the initial post-injury induced hypersensitivity in both SNIs. In tibial-SNI at Day 86, but not at Day 23

  18. Kinetika vulkanisasi campuran NR/EPDM dengan pendekatan model Deng-Isayev dan Kamal-Sorour

    Directory of Open Access Journals (Sweden)

    Ihda Novia Indrajati

    2014-12-01

    Full Text Available Curing kinetics of natural rubber/ethylene propylene diene monomer (NR/EPDM blends at different ratios of 2,2’-dithiobis(benzothiazole (MBTS and zinc diethyl dithiocarbamate (ZDEC were studied. NR/EPDM blends were prepared using curative migration control technique. Kinetics studies employ Deng-Isayev and Kamal-Sorour models and kinetics parameters were evaluated using measured data from Moving Die Rheometer. Rate constant (k and orders of reaction (n and m were estimated through multivariable optimization using Matlab and activation energy (E was determined using Arrhenius relationship. From the results, it is evident that the experimental data were in a good agreement with Kamal-Sorour model. It is also found that autocatalytic mechanism was more controlling than the others as indicated by k2>k1. Results also shows that rate constants were greatly influenced by temperature and the types of accelerator, while orders of reaction were dependent on temperature and independent on the types and amounts of accelerator. Combination of MBTS and ZDEC reduces energy activation of autocatalytic reaction. For desirable rate of curing, its ratio of 1.4 to 0.2 gave the lowest activation energy.

  19. Characterization of vNr-13, the first alphaherpesvirus gene of the bcl-2 family

    International Nuclear Information System (INIS)

    Aouacheria, Abdel; Banyai, Michelle; Rigal, Dominique; Schmidt, Carl J.; Gillet, Germain

    2003-01-01

    The Bcl-2 family, including antiapoptotic and proapoptotic members, plays key regulating roles in programmed cell death. We report the characterization of a new member of the bcl-2 family, encoded by herpesvirus of turkeys (HVT). The product of this gene shares 80% homology with Nr-13, an apoptosis inhibitor, which is overexpressed in avian cells transformed by the v-src oncogene. This new gene, that we propose to call vnr-13, is the first member of the bcl-2 family to be isolated among α-herpesviruses. Results from cells expressing the HVT-vnr-13 gene product show that the encoded protein inhibits apoptosis and also reduces the rate of cellular proliferation. Contrary to all bcl-2 homologues found in γ-herpesvirus, which are intronless, vnr-13 has the same organization as the cellular nr-13 gene. Hence, the HVT vnr-13 gene may have been acquired from a reverse transcriptase product of an unspliced precursor RNA, or via direct recombination with the host chromosomal DNA

  20. Anti-Inflammatory Activity of Polysaccharide Fraction of Curcuma longa Extract (NR-INF-02).

    Science.gov (United States)

    Illuri, Ramanaiah; Bethapudi, Bharathi; Anandakumar, Senthilkumar; Murugan, Sasikumar; Joseph, Joshua A; Mundkinajeddu, Deepak; Agarwal, Amit; Chandrasekaran, C V

    2015-01-01

    The aim of the study was to investigate the safety and anti-inflammatory effects of polysaccharide fraction (F1) of Curcuma longa extract (NR-INF-02) in classical rodent models of inflammation. F1 was evaluated for its acute oral toxicity and found to be safe upto 5000 mg/kg body weight in rats. The anti-inflammatory activity of F1 was evaluated in acute (carrageenan - induced paw edema; xylene - induced ear edema) and chronic (cotton pellet - induced granuloma) models of inflammation. The results of the study demonstrated that F1 significantly (p ≤ 0.05) inhibited carrageenan-induced paw edema at 1 h and 3 h at doses of 11.25, 22.5 and 45 mg/kg body weight in rats. Also, F1 at doses of 15.75, 31.5 and 63 mg/kg significantly inhibited the xylene induced ear edema in mice. In a chronic model, F1 at 11.25, 22.5 and 45 mg/kg doses produced significant reduction of wet and dry weights of cotton pellets in rats. Overall results indicated that F1 of NR-INF-02 significantly attenuated acute and chronic inflammation in rodent models. This study emphasizes on the importance of Curcuma longa polysaccharide's role in acute and chronic inflammation.

  1. Radiation cross-linking of small electrical wire insulator fabricated from NR/LDPE blends

    Energy Technology Data Exchange (ETDEWEB)

    Siri-Upathum, Chyagrit [Department of Nuclear Technology, Faculty of Engineering Chulalongkorn University, Bangkok 10330 (Thailand)], E-mail: chyagrit@chula.ac.th; Punnachaiya, Suvit [Department of Nuclear Technology, Faculty of Engineering Chulalongkorn University, Bangkok 10330 (Thailand)

    2007-12-15

    A low voltage, radiation-crosslinked wire insulator has been fabricated from blends of natural rubber block (STR-5L) and LDPE with phthalic anhydride (PA) as a compatibilizer. Physical properties of the NR/LDPE blend ratios of 50/50 and 60/40 with 0.5, 1.0, and 1.5 wt% PA were evaluated. The gel content increased as the radiation dose increased. Tensile at break exhibited a maximum value of 12 MPa at 120 kGy for 1.0 and 1.5 wt% PA of both blend ratios. A higher PA content yielded a higher modulus for the same blend ratio. Blends of 60/40 ratio with 1.0 wt% PA and 0.8 wt% antimony oxide flame retardant gave the highest limiting oxygen index (LOI) of >30% at above 150 kGy. Other electrical properties of the wire insulator were investigated. It was found that an insulator fabricated from a PA content of 1.0 wt% in the NR/LDPE blend ratio of 50/50, after gamma ray cross-linked at a dose of 180 kGy in low vacuum (1 mm Hg), met the Thai Industrial Standard 11-2531 for low voltage wire below 1.0 kV. To comply with the standard for vertical flame test, a more suitable flame retardant was needed for the insulator.

  2. iNR-PhysChem: a sequence-based predictor for identifying nuclear receptors and their subfamilies via physical-chemical property matrix.

    Directory of Open Access Journals (Sweden)

    Xuan Xiao

    Full Text Available Nuclear receptors (NRs form a family of ligand-activated transcription factors that regulate a wide variety of biological processes, such as homeostasis, reproduction, development, and metabolism. Human genome contains 48 genes encoding NRs. These receptors have become one of the most important targets for therapeutic drug development. According to their different action mechanisms or functions, NRs have been classified into seven subfamilies. With the avalanche of protein sequences generated in the postgenomic age, we are facing the following challenging problems. Given an uncharacterized protein sequence, how can we identify whether it is a nuclear receptor? If it is, what subfamily it belongs to? To address these problems, we developed a predictor called iNR-PhysChem in which the protein samples were expressed by a novel mode of pseudo amino acid composition (PseAAC whose components were derived from a physical-chemical matrix via a series of auto-covariance and cross-covariance transformations. It was observed that the overall success rate achieved by iNR-PhysChem was over 98% in identifying NRs or non-NRs, and over 92% in identifying NRs among the following seven subfamilies: NR1--thyroid hormone like, NR2--HNF4-like, NR3--estrogen like, NR4--nerve growth factor IB-like, NR5--fushi tarazu-F1 like, NR6--germ cell nuclear factor like, and NR0--knirps like. These rates were derived by the jackknife tests on a stringent benchmark dataset in which none of protein sequences included has ≥60% pairwise sequence identity to any other in a same subset. As a user-friendly web-server, iNR-PhysChem is freely accessible to the public at either http://www.jci-bioinfo.cn/iNR-PhysChem or http://icpr.jci.edu.cn/bioinfo/iNR-PhysChem. Also a step-by-step guide is provided on how to use the web-server to get the desired results without the need to follow the complicated mathematics involved in developing the predictor. It is anticipated that iNR-PhysChem may

  3. Development of radiotracers for imaging NR2B subtype NMDA receptors with positron emission tomography; Developpement de radiotraceurs pour la visualisation des recepteurs NMDA de sous-type NR2B par tomographie par emission de positons

    Energy Technology Data Exchange (ETDEWEB)

    Labas, R

    2007-07-01

    The aim of this thesis was to develop new radioactive tracers for imaging NR2B subtype NMDA receptors with positron emission tomography. Several compounds including 4-(4-fluoro-benzyl)piperidine and presenting interesting in vivo biological properties were the object of a labelling with a positrons emitter atom ({sup 11}C or {sup 18}F)

  4. Criteria for assembly of in vivo measuring systems using high-resolution {gamma}-spectroscopy for evaluation of incorporated radionuclides; Kriterien zum Aufbau von In Vivo Messsystemen zur hochaufloesenden {gamma}-Spektrometrie inkorporierter Radionuklide

    Energy Technology Data Exchange (ETDEWEB)

    Wahl, W. [GSF Forschungszentrum fuer Umwelt und Gesundheit, Neuherberg (Germany). Inst. fuer Strahlenschutz

    1997-12-01

    The paper reviews the available, fundamental measuring methods relying on {gamma}-spectroscopy for their possible application in whole-body and partial-body counters for detection of manifold incorporation of radionuclides. Particular emphasis is placed on the response functions of various detectors, the assembly, the differentiated radioactivity distribution in the body, the various components of background activity and the corresponding suppression mechanisms, and possible ways of using the energy dependence for optimised detection of specific {gamma} energies in a given body region. Criteria and relations as well as their advantages and drawbacks are discussed. (orig./CB) [Deutsch] Diese Arbeit prueft die zur Verfuegung stehenden grundlegenden, {gamma}-spektroskopischen Messmethoden auf deren moegliche Anwendung im Spektrum der Ganz- und Teilkoerperzaehler zum Nachweis der mannigfach inkorporierten Radionuklide. Insbesondere wird eingegangen auf die Response Funktionen verschiedener Detektoren, die Anordnung, die differenzierte Aktivitaetsverteilung im Koerper, die verschiedenen Untergrundkomponenten und deren Unterdrueckungsmechanismen sowie die Beeinflussung durch die Energieabhaengigkeit zum optimalen Nachweis spezifischer {gamma}-Energien an einem bestimmten Ort am Koerper. Kriterien und Relationen sowie deren Vor- und Nachteile werden diskutiert. (orig.)

  5. 27 January 2012 - Mitglieder des Stiftungsrates Academia Engelberg und Gesellschaft zum Bettag Luzern Schweiz welcomed by Head of International Relations F. Pauss; visiting LHC tunnel at Point 5 and CMS experimental cavern; in the LHC superconducting magnet test hall SM18.

    CERN Multimedia

    Maximilien Brice

    2012-01-01

    27 January 2012 - Mitglieder des Stiftungsrates Academia Engelberg und Gesellschaft zum Bettag Luzern Schweiz welcomed by Head of International Relations F. Pauss; visiting LHC tunnel at Point 5 and CMS experimental cavern; in the LHC superconducting magnet test hall SM18.

  6. Paweł Bąk, Die Metapher in der Übersetzung. Studien zum Transfer der Aphorismen von Stanisław Jerzy Lec und der Gedichte von Wisława Szymborska, Peter Lang Europäischer Verlag der Wissenschaft en, Frankfurt am Main u. a. 2007 =...

    OpenAIRE

    Zieliński, Lech

    2008-01-01

    Paweł Bąk, Die Metapher in der Ubersetzung. Studien zum Transfer der Aphorismen von Stanisław Jerzy Lec und der Gedichte von Wisława Szymborska, Peter Lang Europaischer Verlag der Wissenschaft en, Frankfurt am Main u. a. 2007 = Danziger Beitrage zur Germanistik, Bd. 20 wyd. Andrzej Kątny, 332 strony

  7. Long-term potentiation in the CA1 hippocampus induced by NR2A subunit-containing NMDA glutamate receptors is mediated by Ras-GRF2/Erk map kinase signaling.

    Directory of Open Access Journals (Sweden)

    Shan-xue Jin

    Full Text Available BACKGROUND: NMDA-type glutamate receptors (NMDARs are major contributors to long-term potentiation (LTP, a form of synaptic plasticity implicated in the process of learning and memory. These receptors consist of calcium-permeating NR1 and multiple regulatory NR2 subunits. A majority of studies show that both NR2A and NR2B-containing NMDARs can contribute to LTP, but their unique contributions to this form of synaptic plasticity remain poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we show that NR2A and NR2B-containing receptors promote LTP differently in the CA1 hippocampus of 1-month old mice, with the NR2A receptors functioning through Ras-GRF2 and its downstream effector, Erk Map kinase, and NR2B receptors functioning independently of these signaling molecules. CONCLUSIONS/SIGNIFICANCE: This study demonstrates that NR2A-, but not NR2B, containing NMDA receptors induce LTP in pyramidal neurons of the CA1 hippocampus from 1 month old mice through Ras-GRF2 and Erk. This difference add new significance to the observation that the relative levels of these NMDAR subtypes is regulated in neurons, such that NR2A-containing receptors become more prominent late in postnatal development, after sensory experience and synaptic activity.

  8. 40 CFR 80.572 - What labeling requirements apply to retailers and wholesale purchaser-consumers of NR and NRLM...

    Science.gov (United States)

    2010-07-01

    ... PROGRAMS (CONTINUED) REGULATION OF FUELS AND FUEL ADDITIVES Motor Vehicle Diesel Fuel; Nonroad, Locomotive, and Marine Diesel Fuel; and ECA Marine Fuel Labeling Requirements § 80.572 What labeling requirements... retailers and wholesale purchaser-consumers of NR and NRLM diesel fuel and heating oil beginning June 1...

  9. Sibelius. Lemminkäinen-Legenden op. 22 Nr. 1-4 / Christoph Schlüren

    Index Scriptorium Estoniae

    Schlüren, Christoph

    1997-01-01

    Uuest heliplaadist "Sibelius: Lemminkäinen-Legenden op. 22 Nr. 1-4, Nächtlicher Ritt und Sonnenaufgang op. 55, Luonnotar op. 70. Stockholm Philharmoniker / Paavo Järvi. Virgin/EMI CD 545213 2 (WD:70'22")DDD

  10. Complete chloroplast genome and 45S nrDNA sequences of the medicinal plant species Glycyrrhiza glabra and Glycyrrhiza uralensis.

    Science.gov (United States)

    Kang, Sang-Ho; Lee, Jeong-Hoon; Lee, Hyun Oh; Ahn, Byoung Ohg; Won, So Youn; Sohn, Seong-Han; Kim, Jung Sun

    2017-10-06

    Glycyrrhiza uralensis and G. glabra, members of the Fabaceae, are medicinally important species that are native to Asia and Europe. Extracts from these plants are widely used as natural sweeteners because of their much greater sweetness than sucrose. In this study, the three complete chloroplast genomes and five 45S nuclear ribosomal (nr)DNA sequences of these two licorice species and an interspecific hybrid are presented. The chloroplast genomes of G. glabra, G. uralensis and G. glabra × G. uralensis were 127,895 bp, 127,716 bp and 127,939 bp, respectively. The three chloroplast genomes harbored 110 annotated genes, including 76 protein-coding genes, 30 tRNA genes and 4 rRNA genes. The 45S nrDNA sequences were either 5,947 or 5,948 bp in length. Glycyrrhiza glabra and G. glabra × G. uralensis showed two types of nrDNA, while G. uralensis contained a single type. The complete 45S nrDNA sequence unit contains 18S rRNA, ITS1, 5.8S rRNA, ITS2 and 26S rRNA. We identified simple sequence repeat and tandem repeat sequences. We also developed four reliable markers for analysis of Glycyrrhiza diversity authentication.

  11. Medtner: Konzert für Klavier und Orchester Nr. 1 c-Moll op. 33 / Volkmar Fischer

    Index Scriptorium Estoniae

    Fischer, Volkmar

    1992-01-01

    Uuest heliplaadist "Medtner: Konzert für Klavier und Orchester Nr. 1 c-Moll op. 33, Sonaten-Ballade für Klavier Fis-Dur op. 27. London Philharmonic Orchestra / Neeme Järvi". Chandos/Koch CD9039 (WD:54'28")

  12. Down-regulation of NR2B receptors partially contributes to analgesic effects of Gentiopicroside in persistent inflammatory pain.

    Science.gov (United States)

    Chen, Lei; Liu, Jin-cheng; Zhang, Xiao-nan; Guo, Yan-yan; Xu, Zhao-hui; Cao, Wei; Sun, Xiao-li; Sun, Wen-ji; Zhao, Ming-Gao

    2008-06-01

    Gentiopicroside is one of the secoiridoid compound isolated from Gentiana lutea. It exhibits analgesic activities in the mice. The anterior cingulate cortex (ACC) is a forebrain structure known for its roles in pain transmission and modulation. Painful stimuli potentiate the prefrontal synaptic transmission and induce glutamate NMDA NR2B receptor expression in the ACC. But little is known about Gentiopicroside on the persistent inflammatory pain and chronic pain-induced synaptic transmission changes in the ACC. The present study was undertaken to investigate its analgesic activities and central synaptic modulation to the peripheral painful inflammation. Gentiopicroside produced significant analgesic effects against persistent inflammatory pain stimuli in mice. Systemic administration of Gentiopicroside significantly reversed NR2B over-expression during the chronic phases of persistent inflammation caused by hind-paw administration of complete Freunds adjuvant (CFA) in mice. Whole-cell patch clamp recordings revealed that Gentiopicroside significantly reduced NR2B receptors mediated postsynaptic currents in the ACC. Our findings provide strong evidence that analgesic effects of Gentiopicroside involve down-regulation of NR2B receptors in the ACC to persistent inflammatory pain.

  13. Carbon monoxide alleviates lipopolysaccharide-induced oxidative stress injury through suppressing the expression of Fis1 in NR8383 cells

    Energy Technology Data Exchange (ETDEWEB)

    Shi, Jia [Department of Anesthesiology, Tianjin Nankai Hospital, Tianjin Medical University, Tianjin 300100 (China); Yu, Jian-bo, E-mail: yujianbo11@126.com [Department of Anesthesiology, Tianjin Nankai Hospital, Tianjin Medical University, Tianjin 300100 (China); Liu, Wei; Wang, Dan; Zhang, Yuan; Gong, Li-rong; Dong, Shu-an [Department of Anesthesiology, Tianjin Nankai Hospital, Tianjin Medical University, Tianjin 300100 (China); Liu, Da-quan [Department of Pharmacology, Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100 (China)

    2016-11-15

    Acute respiratory distress syndrome (ARDS) is one of the most devastating complications of sepsis lacking of effective therapy. Mitochondrial dynamics undergoing continuous fusion and fission play a crucial role in mitochondrial structure and function. Fis1, as a small protein located on the outer membrane of mitochondria, has been thought to be an important protein mediated mitochondrial fission. During ARDS, alveolar macrophages suffer from increased oxidative stress and apoptosis, and also accompanied by disrupted mitochondrial dynamics. In addition, as one of the products of heme degradation catalyzed by heme oxygenase, carbon monoxide (CO) possesses powerful protective properties in vivo or in vitro models, such as anti-inflammatory, antioxidant and anti-apoptosis function. However, there is little evidence that CO alleviates oxidative stress damage through altering mitochondrial fission in alveolar macrophages. In the present study, our results showed that CO increased cell vitality, improved mitochondrial SOD activity, reduced reactive oxygen species (ROS) production and inhibited cell apoptosis in NR8383 exposed to LPS. Meanwhile, CO decreased the expression of Fis1, increased mitochondrial membrane potential and sustained elongation of mitochondria in LPS-incubated NR8383. Overall, our study underscored a critical role of CO in suppressing the expression of Fis1 and alleviating LPS- induced oxidative stress damage in alveolar macrophages. - Highlights: • LPS exposure triggered cell injury in NR8383. • CO alleviated LPS-induced oxidative stress damage in alveolar macrophages. • CO inhibited Fis1 levels and improved mitochondrial function in LPS-induced NR8383.

  14. The Intermediate-mass Young Stellar Object 08576nr292: Discovery of A Disk-Jet System

    NARCIS (Netherlands)

    Ellerbroek, L.E.; Kaper, L.; Bik, A.; de Koter, A.; Horrobin, M.; Puga, E.; Sana, H.; Waters, L.B.F.M.

    2011-01-01

    We present observations of the embedded massive young stellar object (YSO) candidate 08576nr292, obtained with X-shooter and SINFONI on the ESO Very Large Telescope (VLT). The flux-calibrated, medium-resolution X-shooter spectrum (300–2500 nm) includes over 300 emission lines, but no (photospheric)

  15. "NR3C1" Methylation as a Moderator of the Effects of Maternal Support and Stress on Insecure Attachment Development

    Science.gov (United States)

    Bosmans, Guy; Young, Jami F.; Hankin, Benjamin L.

    2018-01-01

    We examined the prediction that the interaction between Glucocorticoid Receptor Gene ("NR3C1") methylation, stress, and experienced maternal support predicts anxious and avoidant attachment development. This was tested in a general population sample of 487 children and adolescents (44% boys, M[subscript age] = 11.84, SD[subscript age] =…

  16. Analysis of C. elegans NR2E nuclear receptors defines three conserved clades and ligand-independent functions

    Directory of Open Access Journals (Sweden)

    Weber Katherine P

    2012-06-01

    Full Text Available Abstract Background The nuclear receptors (NRs are an important class of transcription factors that are conserved across animal phyla. Canonical NRs consist of a DNA-binding domain (DBD and ligand-binding domain (LBD. While most animals have 20–40 NRs, nematodes of the genus Caenorhabditis have experienced a spectacular proliferation and divergence of NR genes. The LBDs of evolutionarily-conserved Caenorhabditis NRs have diverged sharply from their Drosophila and vertebrate orthologs, while the DBDs have been strongly conserved. The NR2E family of NRs play critical roles in development, especially in the nervous system. In this study, we explore the phylogenetics and function of the NR2E family of Caenorhabditis elegans, using an in vivo assay to test LBD function. Results Phylogenetic analysis reveals that the NR2E family of NRs consists of three broadly-conserved clades of orthologous NRs. In C. elegans, these clades are defined by nhr-67, fax-1 and nhr-239. The vertebrate orthologs of nhr-67 and fax-1 are Tlx and PNR, respectively. While the nhr-239 clade includes orthologs in insects (Hr83, an echinoderm, and a hemichordate, the gene appears to have been lost from vertebrate lineages. The C. elegans and C. briggsae nhr-239 genes have an apparently-truncated and highly-diverged LBD region. An additional C. elegans NR2E gene, nhr-111, appears to be a recently-evolved paralog of fax-1; it is present in C. elegans, but not C. briggsae or other animals with completely-sequenced genomes. Analysis of the relatively unstudied nhr-111 and nhr-239 genes demonstrates that they are both expressed—nhr-111 very broadly and nhr-239 in a small subset of neurons. Analysis of the FAX-1 LBD in an in vivo assay revealed that it is not required for at least some developmental functions. Conclusions Our analysis supports three conserved clades of NR2E receptors, only two of which are represented in vertebrates, indicating three ancestral NR2E genes in the

  17. Combined serial analysis of gene expression and transcription factor binding site prediction identifies novel-candidate-target genes of Nr2e1 in neocortex development.

    Science.gov (United States)

    Schmouth, Jean-François; Arenillas, David; Corso-Díaz, Ximena; Xie, Yuan-Yun; Bohacec, Slavita; Banks, Kathleen G; Bonaguro, Russell J; Wong, Siaw H; Jones, Steven J M; Marra, Marco A; Simpson, Elizabeth M; Wasserman, Wyeth W

    2015-07-24

    Nr2e1 (nuclear receptor subfamily 2, group e, member 1) encodes a transcription factor important in neocortex development. Previous work has shown that nuclear receptors can have hundreds of target genes, and bind more than 300 co-interacting proteins. However, recognition of the critical role of Nr2e1 in neural stem cells and neocortex development is relatively recent, thus the molecular mechanisms involved for this nuclear receptor are only beginning to be understood. Serial analysis of gene expression (SAGE), has given researchers both qualitative and quantitative information pertaining to biological processes. Thus, in this work, six LongSAGE mouse libraries were generated from laser microdissected tissue samples of dorsal VZ/SVZ (ventricular zone and subventricular zone) from the telencephalon of wild-type (Wt) and Nr2e1-null embryos at the critical development ages E13.5, E15.5, and E17.5. We then used a novel approach, implementing multiple computational methods followed by biological validation to further our understanding of Nr2e1 in neocortex development. In this work, we have generated a list of 1279 genes that are differentially expressed in response to altered Nr2e1 expression during in vivo neocortex development. We have refined this list to 64 candidate direct-targets of NR2E1. Our data suggested distinct roles for Nr2e1 during different neocortex developmental stages. Most importantly, our results suggest a possible novel pathway by which Nr2e1 regulates neurogenesis, which includes Lhx2 as one of the candidate direct-target genes, and SOX9 as a co-interactor. In conclusion, we have provided new candidate interacting partners and numerous well-developed testable hypotheses for understanding the pathways by which Nr2e1 functions to regulate neocortex development.

  18. NR2F2 inhibits Smad7 expression and promotes TGF-β-dependent epithelial-mesenchymal transition of CRC via transactivation of miR-21.

    Science.gov (United States)

    Wang, Hao; Nie, Lei; Wu, Lei; Liu, Qiufang; Guo, Xueyan

    2017-03-25

    Metastasis is one of the most decisive factors influencing CRC patient prognosis and current studies suggest that a molecular mechanism known as EMT broadly regulates cancer metastasis. NR2F2 is a key molecule in the development of CRC, but the roles and underlying mechanisms of NR2F2 in TGF-β induced EMT in CRC remain largely unknown. In the current study, we were interested to examine the role of NR2F2 in the TGF-β-induced EMT in CRC. Here, we found NR2F2 was upregulated in CRC cells and promotes TGF-β-induced EMT in CRC. Using comparative miRNA profiling TGF-β pre-treated CRC cells in which NR2F2 had been knocked down with that of control cells, we identified miR-21 as a commonly downregulated miRNA in HT29 cells treated with TGF-β and NR2F2 siRNA, and its downregulation inhibiting migration and invasion of CRC cells. Moreover, we found NR2F2 could transcriptional activated miR-21 expression by binding to miR-21 promoter in HT29 by ChIP and luciferase assay. In the last, our data demonstrated that Smad7 was the direct target of miR-21 in CRC cells. Thus, NR2F2 could promote TGF-β-induced EMT and inhibit Smad7 expression via transactivation of miR-21, and NR2F2 may be a new common therapeutic target for CRC. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. GLYX-13 Ameliorates Schizophrenia-Like Phenotype Induced by MK-801 in Mice: Role of Hippocampal NR2B and DISC1

    Science.gov (United States)

    Zhou, Dongsheng; Lv, Dan; Wang, Zhen; Zhang, Yanhua; Chen, Zhongming; Wang, Chuang

    2018-01-01

    Background: Evidence supports that the hypofunction of N-methyl-D-aspartate receptor (NMDAR) and downregulation of disrupted-in-schizophrenia 1 (DISC1) contribute to the pathophysiology of schizophrenia. N-Methyl D-aspartate receptor subtype 2B (NR2B)-containing NMDAR are associated with cognitive dysfunction in schizophrenia. GLYX-13 is an NMDAR glycine-site functional partial agonist and cognitive enhancer that does not induce psychotomimetic side effects. However, it remains unclear whether NR2B plays a critical role in the GLYX-13-induced alleviation of schizophrenia-like behaviors in mice. Methods: The effect of GLYX-13 was tested by observing changes in locomotor activity, novel object recognition ability, and prepulse inhibition (PPI) induced by dizocilpine (known as MK-801) in mice. Lentivirus-mediated NR2B knockdown in the hippocampus was assessed to confirm the role of NR2B in GLYX-13 pathophysiology, using Western blots and immunohistochemistry. Results: The systemic administration of GLYX-13 (0.5 and 1 mg/kg, i.p.) ameliorates MK-801 (0.5 mg/kg, i.p.)-induced hyperlocomotion, deficits in memory, and PPI in mice. Additionally, GLYX-13 normalized the MK-801-induced alterations in signaling molecules, including NR2B and DISC1 in the hippocampus. Furthermore, we found that NR2B knockdown produced memory and PPI deficits without any changes in locomotor activity. Notably, DISC1 levels significantly decreased by NR2B knockdown. However, the effective dose of GLYX-13 did not alleviate the memory and PPI dysfunctions or downregulation of DISC1 induced by NR2B knockdown. Conclusion: Our results suggest GLYX-13 as a candidate for schizophrenia treatment, and NR2B and DISC1 in the hippocampus may account for the molecular mechanisms of GLYX-13. PMID:29695955

  20. Relevance of the NR4A sub-family of nuclear orphan receptors in trophoblastic BeWo cell differentiation.

    Science.gov (United States)

    Malhotra, Sudha Saryu; Gupta, Satish Kumar

    2017-01-01

    Nur-77, a member of the NR4A sub-family of nuclear orphan receptors, is downregulated in the placentae of pre-eclamptic women. Here, we investigate the relevance of Nor-1, Nurr-1 and Nur-77 in trophoblastic cell differentiation. Their transcript levels were found to be significantly upregulated in BeWo cells treated with forskolin. The maximum increase was observed after 2 h, with a second peak in the expression levels after 48 h. The expression of NR4A sub-family members was also found to be upregulated in BeWo cells after treatment with hCG and GnRH. A similar significant increase was observed at the respective protein levels after 2 and 48 h of treatment with forskolin, hCG or GnRH. Silencing Nor-1, Nurr-1 or Nur-77 individually did not show any effect on forskolin-, hCG- and/or GnRH-mediated BeWo cell fusion and/or hCG secretion. After silencing any one member of the NR4A sub-family, an increase in the transcript levels of the other sub-family members was observed, indicating a compensatory effect due to their functional redundancy. Simultaneously silencing all three NR4A sub-family members significantly downregulated forskolin- and hCG-mediated BeWo cell fusion and/or hCG secretion. However, a considerable amount of cell death occurred after forskolin or hCG treatment as compared to the control siRNA-transfected cells. These results suggest that the NR4A sub-family of nuclear orphan receptors has a role in trophoblastic cell differentiation.

  1. PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO

    Directory of Open Access Journals (Sweden)

    Edgar Antonio Reyes Montaño

    2011-12-01

    Full Text Available Producing polyclonal antibodies (IgY inchickens has advantages over those obtainedin other animal models, since theyhave been used as a tool for studyingdifferent proteins (NMDA glutamate receptorin our case, specifically the NR1subunit. We produced specific antibodiesagainst expression products by thealternative splicing of the gene encodingNMDA receptor NR1 subunit in adult ratbrain. Three peptides corresponding tothe splicing sites (N1, C1 and C2’ cassetteswere designed, synthesised and usedindividually as antigens in hens. Specificimmunoglobulins were purified fromyolks. The antibodies were then used forpurifying the NMDA receptor NR1 subunitusing affinity chromatography couplingthe three antibodies to the support.R

  2. Biodistribution, pharmacokinetic, and imaging studies with 186Re-labeled NR-LU-10 whole antibody in LS174T colonic tumor-bearing mice

    International Nuclear Information System (INIS)

    Goldrosen, M.H.; Biddle, W.C.; Pancook, J.; Bakshi, S.; Vanderheyden, J.L.; Fritzberg, A.R.; Morgan, A.C. Jr.; Foon, K.A.

    1990-01-01

    Biodistribution, pharmacokinetic, and radioimaging studies were performed with 186Re-labeled NR-LU-10 whole antibody in athymic nude mice bearing the LS174T tumor growing either s.c. or in an experimental hepatic metastasis model. NR-LU-10 is an IgG2b murine monoclonal antibody (MAb) that reacts with virtually all human tumors of epithelial origin. NR-BC-1, a IgG2b murine MAb that reacts with normal human B-cell and B malignancies, was used as an isotype-matched control. These MAbs were radiolabeled with 186Re by a preformed chelate approach by using the triamide thiolate ligand system. 186Re-labeled NR-LU-10 (50 microCi) was injected into nude mice bearing LS174T tumors growing s.c. Biodistribution studies revealed that the LS174T tumor retained the highest concentration of 186Re-labeled NR-LU-10 at day 6. The tumor:blood ratio ranged from 0.1:1 to 10.8:1 by day 6, the last day of analysis. In contrast the tumor:blood ratio of 186Re-labeled NR-BC-1, the isotype-matched MAb control, was 1:1 on day 6. Pharmacokinetic analysis indicated that the t1/2 beta of NR-LU-10 for blood and other tissues ranged from 21 to 25 h, while the t1/2 beta for the LS174T tumor averaged 52 h. The area under the curve for tumor compared to blood was 2.8- to 5.7-fold higher than the area under the curve for all other tissues and organs. The mean residence time for NR-LU-10 in blood and all other organs ranged from 23 to 26 h, while the mean residence time for NR-LU-10 in the LS174T tumor was 72 h. Scintigraphic images revealed selective uptake of the 186Re-labeled NR-LU-10, but not of the 186Re-labeled NR-BC-1, at the LS174T tumor site. Studies in an experimental model of hepatic metastasis revealed a similar selective pattern of 186Re-labeled NR-LU-10 accumulation. Scintigraphic images of the LS174T tumor growing within the athymic nude mouse liver were obtained

  3. Modulation of expression of the nuclear receptor NR0B2 (small heterodimer partner 1 and its impact on proliferation of renal carcinoma cells

    Directory of Open Access Journals (Sweden)

    Prestin K

    2016-08-01

    Full Text Available Katharina Prestin,1,* Maria Olbert,2,* Janine Hussner,1 Tamara L Isenegger,1 Daniel G Gliesche,1 Kerstin Böttcher,2 Uwe Zimmermann,3 Henriette E Meyer zu Schwabedissen1 1Department of Pharmaceutical Sciences, Biopharmacy, University of Basel, Basel, Switzerland; 2Center of Drug Absorption and Transport, Institute of Pharmacology, 3Department of Urology, University Medicine Greifswald, Greifswald, Germany *These authors contributed equally to this work Abstract: Mammalian nuclear receptors (NRs are transcription factors regulating the expression of target genes that play an important role in drug metabolism, transport, and cellular signaling pathways. The orphan and structurally unique receptor small heterodimer partner 1 (syn NR0B2 is not only known for its modulation of drug response, but has also been reported to be involved in hepatocellular carcinogenesis. Indeed, previous studies show that NR0B2 is downregulated in human hepatocellular carcinoma, suggesting that NR0B2 acts as a tumor suppressor via inhibition of cellular growth and activation of apoptosis in this tumor entity. The aim of our study was to elucidate whether NR0B2 may also play a role in other tumor entities. Comparing NR0B2 expression in renal cell carcinoma and adjacent nonmalignant transformed tissue revealed significant downregulation in vivo. Additionally, the impact of heterologous expression of NR0B2 on cell cycle progression and proliferation in cells of renal origin was characterized. Monitoring fluorescence intensity of resazurin turnover in RCC-EW cells revealed no significant differences in metabolic activity in the presence of NR0B2. However, there was a significant decrease of cellular proliferation in cells overexpressing this NR, and NR0B2 was more efficient than currently used antiproliferative agents. Furthermore, flow cytometry analysis showed that heterologous overexpression of NR0B2 significantly reduced the amount of cells passing the G1 phase, while on

  4. Alexander von Humboldt und Carlos Montúfar als Reisegefährten: ein Vergleich ihrer Tagebücher zum Chimborazo-Aufstieg

    Directory of Open Access Journals (Sweden)

    Reinhard Andress

    2011-03-01

    Full Text Available ZusammenfassungAls Alexander von Humboldt im Juni 1802 von Quito aus zum Rest seiner lateinamerikanischen Forschungsreise aufbrach, begleitete ihn, abgesehen von Aimé Bonpland, Carlos Montúfar bis nach Europa zurück begleitet, u.a. auch auf beim Versuch, den Chimborazo zu ersteigen. Bis kurz vor der Stadt Cajamarca im heutigen Peru schrieb Montúfar ein relativ unbekanntes Tagebuch zur Reise. Nach einem kurzen Ausflug in das Leben von Humboldts Begleiter steht im Zentrum der weiteren Ausführungen die Frage, ob sein Tagebuch ein anderes Licht auf Humboldt und speziell auf den Aufstiegsversuch wirft. Das läuft auf einen Vergleich der Tagebücher der beiden Reisegefährten hinaus und auf den Versuch, die scheinbaren Widersprüche aufzulösen.AbstractWhen Alexander von Humboldt left Quito in June 1802 in order to undertake the rest of his Latin-American trip of exploration, he was accompanied, aside from Aimé Bonpland, by Carlos Montúfar all the way back to Europe, including the attempt to scale the Chimborazo. Up to shortly before the city of Cajamarca in today’s Peru, Montúfar wrote an relatively unknown diary account of their travels. After a brief excursion into the life of Humboldt’s companion, the question is considered whether his diary sheds a different light on Humboldt and specifically on the mountain climb. That amounts to a comparison of the diaries of the two travel companions and to an attempt to explain the apparent contradictions.ResumenCuando Alexander von Humboldt salió de Quito en junio de 1802 para continuar con el resto de su viaje de exploración por la América Latina, fue acompañado por Carlos Montúfar además de Aimé Bonpland hasta que volvieron a Europa. Este trayecto incluía la tentativa de llegar a la cima del Chimborazo. Hasta un poco antes de la cuidad de Cajamarca que está en el Perú de hoy día, Montúfar mantenía un diario relativamente no conocido del viaje. Después de una breve excursión por

  5. (Kein Grund zur Homosexualität: Ein Plädoyer zum Verzicht auf psychogenetische Erklärungsversuche von homosexuellen, heterosexuellen und anderen Orientierungen

    Directory of Open Access Journals (Sweden)

    Ralf Binswanger

    2016-07-01

    Full Text Available Fritz Morgenthaler steht mit seinem Konzept der «Weichenstellungen » auf dem Boden von Freuds Postulat, wonach homosexuelle und heterosexuelle Orientierungen gleich erklärungsbedürftig sind. Allerdings können die «Weichenstellungen» weniger Hetero- oder Homosexualität per se erklären, sondern eher die verschiedenen Formen von Homo- und Heterosexualität in actu, wie sie in Fantasie und Verhalten gelebt werden. Mit Sexualität per se und Sexualität in actu sind zwei qualitativ verschiedene Gesichtspunkte benannt, unter denen Diskurse über manifeste und organisierte Sexualität sowie Perversionen mit Vorteil geführt werden. Dabei wird der Perversionsbegriff ausschliesslich der Sexualität in actu zugeordnet, welche als dialektische Einheit von sexuellen und nicht-sexuellen Funktionen aufgefasst wird. Pervers ist sexuelle Aktivität dann, wenn innerhalb dieser Einheit eine nicht-sexuelle Funktion die Priorität über die Triebbefriedigung hat. Sexualität per se bezeichnet Homosexualität, Heterosexualität, Fetischismus, Sadomasochismus, Pädophilie usw. als verschiedene erwachsene Sexualorganisationen – als hierarchische Organisation von Partialtrieben – auf «gleichberechtigter», entpathologisierter Ebene. Nachdem begründet wird, weshalb auch Judith Le Soldat den Grund zur Homosexualität per se anscheinend nicht gefunden hat und alle bisherigen psychogenetischen Herleitungen der verschiedenen erwachsenen Sexualorganisationen dieses Ziel verfehlen, empfiehlt sich ein grundsätzlicher Verzicht auf solche Erklärungsversuche – so wie es für die Heterosexualität per se meistens gehandhabt wird. Dagegen kann auf den u. a. von Robert Stoller verwendeten Begriff imprinting oder Prägung zurückgegriffen werden, welchen die Zürcher Analytiker Harold Lincke und Werner Fessler von jeglichem Biologismusverdacht befreit haben. Zum Schluss wird hervorgehoben, wie sehr Morgenthaler und Le Soldat mit ihrer Suche nach dem Grund zur

  6. Muscle Contraction Induces Acute Hydroxymethylation of the Exercise-Responsive Gene Nr4a3

    DEFF Research Database (Denmark)

    Pattamaprapanont, Pattarawan; Garde, Christian; Fabre, Odile

    2016-01-01

    stimulated over time is required to determine whether contraction-induced demethylation is preceded by changes in the hydroxymethylcytosine level. Here, we established an acute skeletal muscle contraction model to mimic the effects of acute exercise on gene expression. We used this model to investigate...... promoters. Exercise induces dynamic DNA demethylation at gene promoters; however, the contribution of the demethylation precursor hydroxymethylcytosine is unknown. Given the evanescent nature of hydroxymethylcytosine, a muscle contraction model that allows for the collection of samples that are repeatedly...... the effect of muscle contraction on DNA demethylation and hydroxymethylation. First, we performed an acute exercise study in healthy humans to identify an exercise-responsive gene that we could study in culture. We identified the nuclear receptor subfamily 4 group A member 3 (Nr4a3) gene with the highest...

  7. Masalah dan Cara Mengatasi Sistem Informasi Akuntansi Siklus Pengeluaran Kas di PT NR

    Directory of Open Access Journals (Sweden)

    Engelwati Gani

    2014-11-01

    Full Text Available A problem in accounting information systems of expenditure cash cycle in PT NR is estimated purchase raw materials often left over and valuable. To overcome it, accounting information with computer-based systems was designed related to raw material purchase that is expected to speed up procurement process and avoid fraud and error. The system processes waste materials that will be stored and used properly, so as to increase efficiency in purchasing raw materials. The analysis used for the construction of this system was business process analysis, problem identification, and analysis of information needs. The result achieved is accounting information systems of expenditure cash cycle to overcome the problem existing in the company. Research concludes the use of information systems can speed up purchase process and avoid fraud. Raw material management with system will make it easier to perform the calculation of actual costs and improve internal control.

  8. Radiation Crosslinking of Small Electrical Wire Insulator Fabricated from NR-LDPE Blend

    International Nuclear Information System (INIS)

    Chyagrit, S.

    2006-01-01

    Blending of block natural rubber (STR-5L) and LDPE with phthalic anhydride (PA) as copatibilizer was put to the test for the purpose of a fabrication into small electrical wire insulator. It was found that PA at concentration of 1.0 - 1.5% in NR/PE of 50/50 so fabricated into the insulator, after gamma ray cross-linked at a dose of 180 kGy in limited air, could meet Thai Industrial Standard (TIS) 11-2531 of small eletrical insulator (<300 V). Effect of radiation dose on tensile, hardness, elongation at break, modulus 100%, limiting oxigen index (LOI) were investigated. It was noted that to comply with TIS 11-2531 for vertical flame retardance test, a suitable flame retardance was needed for the insulator

  9. PNNL Apatite Investigation at 100-NR-2 Quality Assurance Project Plan

    Energy Technology Data Exchange (ETDEWEB)

    Fix, N. J.

    2009-04-02

    In 2004, the U.S. Department of Energy, Fluor Hanford, Inc., Pacific Northwest National Laboratory (PNNL), and the Washington Department of Ecology agreed that the long-term strategy for groundwater remediation at the 100-N Area would include apatite sequestration as the primary treatment, followed by a secondary treatment if necessary. Since then, the agencies have worked together to agree on which apatite sequestration technology has the greatest chance of reducing strontium-90 flux to the Columbia River. This Quality Assurance Project Plan provides the quality assurance requirements and processes that will be followed by staff working on the PNNL Apatite Investigation at 100-NR-2 Project. The plan is designed to be used exclusively by project staff.

  10. Partnerstwo publiczno – prywatne w świetle Krajowego Standardu Rachunkowości nr 10

    Directory of Open Access Journals (Sweden)

    Dorota Adamek-Hyska

    2017-09-01

    Full Text Available Partnerstwo publiczno – prywatne w świetle Krajowego Standardu Rachunkowości nr 10 In Poland, the concept of public-private partnership generates many dilemmas in valuation, accounting records and the reporting of essential assets and liabilities. This issue is addressed by National Accounting Standard No. 10, which outlines the principles of PPP accounting in public and private accounting systems. The purpose of this paper is to analyze and evaluate the solutions adopted in National Accounting Standard No. 10 against the background of synthetic PPP characteristics. The research methods used are based on a critical analysis of national legislation, a comparative analysis of national and international accounting standards, and a literature analysis of public-private partnerships, public finances and financial accounting. The paper uses the conclusions from the observations of financial aspects of selected public-private partnership contracts as well as concession contracts for construction works and services in Poland

  11. Aquatic studies at the 100-HR-3 and 100-NR-1 operable units

    International Nuclear Information System (INIS)

    Cushing, C.E.

    1993-04-01

    Pacific Northwest Laboratory initiated a program to characterize selected aquatic biological populations to determine (1) existing levels of inorganic chemical and radionuclide contamination, and (2) the populations' suitability as indicators of chemical releases during cleanup activities at the US Department of Energy's Hanford Site. Following work plans for the ground-water operable units, lower trophic levels in the aquatic habitat (periphyton and caddisfly larvae) were evaluated for contaminants at the 100-HR-3 Operable Unit and 100-NR-1 Operable Unit. The results were evaluated to determine the need for further sampling. If the results showed no significant contamination compared to upriver levels, sampling would be discontinued. The periphyton community appears to be suitable for determining contamination levels. Baseline concentrations for stable chromium were established and will be useful for comparing samples collected when contaminant release is expected. Concentrations of 60 Co, 90 Sr, and 137 Cs in periphyton were essentially below detectable limits, which will also make this community useful in detecting potential releases of radionuclides during cleanup activities. Levels for both stable chromium and radionuclides were essentially below detection limits for caddisfly larvae. Thus, these organisms may be used to monitor suspected contaminant releases from cleanup activities; if concentrations exceed detection limits, they may be related to these activities. Two candidate threatened and endangered species of molluscs occur in the Hanford Reach of the Columbia River. These are the shortface lanx (Fisherola nuttalli), which is a Washington State candidate species, and the Columbia pebblesnail (Fluminicola columbiana), which is both a state and federal candidate species. Specimens of the shortface lanx were observed in the vicinity of N Springs (100-NR-1 Operable Unit); they likely occur throughout this area

  12. HPA axis dysregulation, NR3C1 polymorphisms and glucocorticoid receptor isoforms imbalance in metabolic syndrome.

    Science.gov (United States)

    Martins, Clarissa Silva; Elias, Daniel; Colli, Leandro Machado; Couri, Carlos Eduardo; Souza, Manoel Carlos L A; Moreira, Ayrton C; Foss, Milton C; Elias, Lucila L K; de Castro, Margaret

    2017-03-01

    Metabolic syndrome (MetS) shares several similarities with hypercortisolism. To evaluate hypothalamic-pituitary-adrenal (HPA) axis sensitivity to dexamethasone (DEX), NR3C1 single nucleotide polymorphisms (SNPs), and expression of glucocorticoid receptor (GR) isoforms and cytokines in peripheral immune cells of MetS patients and controls. Prospective study with 40 MetS patients and 40 controls was conducted at the Ribeirão Preto Medical School University Hospital. Plasma and salivary cortisol were measured in basal conditions and after 0.25, 0.5, and 1 mg of DEX given at 2300 h. In addition, p.N363S (rs6195), p.ER22/23EK (rs6189-6190), and BclI (rs41423247) SNPs were evaluated by quantitative polymerase chain reaction allelic discrimination. Exons 3 to 9 and exon/intron boundaries of NR3C1 were sequenced. GR isoforms and cytokines (IL1B, IL2, IL4, IL6, IL8, IL10, IFNγ, TNFα) expression were assessed by quantitative polymerase chain reaction. Plasma and salivary cortisol (nmol/L) after 1-mg DEX were higher in MetS patients compared with controls (PF: 70.2 ± 17.3 vs 37.9 ± 2.6, P = .02, and SF: 4.9 ± 1.7 vs 2.2 ± 0.3, P molecular mechanism of glucocorticoid resistance in MetS. Thus, HPA axis dysregulation might contribute to MetS pathogenesis. Copyright © 2016 John Wiley & Sons, Ltd.

  13. The human orphan nuclear receptor tailless (TLX, NR2E1) is druggable.

    Science.gov (United States)

    Benod, Cindy; Villagomez, Rosa; Filgueira, Carly S; Hwang, Peter K; Leonard, Paul G; Poncet-Montange, Guillaume; Rajagopalan, Senapathy; Fletterick, Robert J; Gustafsson, Jan-Åke; Webb, Paul

    2014-01-01

    Nuclear receptors (NRs) are an important group of ligand-dependent transcriptional factors. Presently, no natural or synthetic ligand has been identified for a large group of orphan NRs. Small molecules to target these orphan NRs will provide unique resources for uncovering regulatory systems that impact human health and to modulate these pathways with drugs. The orphan NR tailless (TLX, NR2E1), a transcriptional repressor, is a major player in neurogenesis and Neural Stem Cell (NSC) derived brain tumors. No chemical probes that modulate TLX activity are available, and it is not clear whether TLX is druggable. To assess TLX ligand binding capacity, we created homology models of the TLX ligand binding domain (LBD). Results suggest that TLX belongs to an emerging class of NRs that lack LBD helices α1 and α2 and that it has potential to form a large open ligand binding pocket (LBP). Using a medium throughput screening strategy, we investigated direct binding of 20,000 compounds to purified human TLX protein and verified interactions with a secondary (orthogonal) assay. We then assessed effects of verified binders on TLX activity using luciferase assays. As a result, we report identification of three compounds (ccrp1, ccrp2 and ccrp3) that bind to recombinant TLX protein with affinities in the high nanomolar to low micromolar range and enhance TLX transcriptional repressive activity. We conclude that TLX is druggable and propose that our lead compounds could serve as scaffolds to derive more potent ligands. While our ligands potentiate TLX repressive activity, the question of whether it is possible to develop ligands to de-repress TLX activity remains open.

  14. The human orphan nuclear receptor tailless (TLX, NR2E1 is druggable.

    Directory of Open Access Journals (Sweden)

    Cindy Benod

    Full Text Available Nuclear receptors (NRs are an important group of ligand-dependent transcriptional factors. Presently, no natural or synthetic ligand has been identified for a large group of orphan NRs. Small molecules to target these orphan NRs will provide unique resources for uncovering regulatory systems that impact human health and to modulate these pathways with drugs. The orphan NR tailless (TLX, NR2E1, a transcriptional repressor, is a major player in neurogenesis and Neural Stem Cell (NSC derived brain tumors. No chemical probes that modulate TLX activity are available, and it is not clear whether TLX is druggable. To assess TLX ligand binding capacity, we created homology models of the TLX ligand binding domain (LBD. Results suggest that TLX belongs to an emerging class of NRs that lack LBD helices α1 and α2 and that it has potential to form a large open ligand binding pocket (LBP. Using a medium throughput screening strategy, we investigated direct binding of 20,000 compounds to purified human TLX protein and verified interactions with a secondary (orthogonal assay. We then assessed effects of verified binders on TLX activity using luciferase assays. As a result, we report identification of three compounds (ccrp1, ccrp2 and ccrp3 that bind to recombinant TLX protein with affinities in the high nanomolar to low micromolar range and enhance TLX transcriptional repressive activity. We conclude that TLX is druggable and propose that our lead compounds could serve as scaffolds to derive more potent ligands. While our ligands potentiate TLX repressive activity, the question of whether it is possible to develop ligands to de-repress TLX activity remains open.

  15. Limited field investigation report for the 100-NR-1 Operable Unit

    International Nuclear Information System (INIS)

    1995-03-01

    This limited field investigation (LFI) report summarizes the data collection and analysis activities conducted during the 100-NR-1 Source Operable Unit LFI and the associated qualitative risk assessment (QRA), and makes recommendations on the continued candidacy of high-priority sites for interim remedial measures (IRM). The results and recommendations presented in this report are generally independent of future land-use scenarios. This report is unique in that it is based on Hanford-specific agreements discussed in the Hanford Federal Facility Agreement and Consent Order (Tri-Party Agreement) (Ecology et al. 1992), the Hanford Site Risk Assessment Methodology (DOE-RL 1994a), the Remedial Investigation Feasibility Study Work Plan for the 100-NR-1 Operable Unit (DOE-RL 1992a), and the Hanford Past-Practice Strategy (HPPS) (DOE-RL 1991), and must be viewed in this context. The HPPS emphasizes initiating and completing waste site cleanup through interim actions. The strategy includes paths for interim decision-making and a final remedy-selection process for the operable unit. A LFI report is required, in accordance with the HPPS, when waste sites are to be considered for IRM and existing data are insufficient to formulate a conceptual model or perform a QRA. The purpose of the report is to identify those sites that are recommended to remain as candidates for IRM, provide a preliminary summary of site characterization studies, refine the conceptual model as needed, identify potential contaminant- and location-specific applicable or relevant and appropriate requirements (ARAR), and provide a qualitative assessment of the risks associated with the sites. This assessment includes consideration of whether contaminant concentrations pose a risk to human health or the environment that warrants action through IRM

  16. Novel de novo pathogenic variant in the NR2F2 gene in a boy with congenital heart defect and dysmorphic features.

    Science.gov (United States)

    Upadia, Jariya; Gonzales, Patrick R; Robin, Nathaniel H

    2018-04-16

    The NR2F2 gene plays an important role in angiogenesis and heart development. Moreover, this gene is involved in organogenesis in many other organs in mouse models. Variants in this gene have been reported in a number of patients with nonsyndromic atrioventricular septal defect, and in one patient with congenital heart defect and dysmorphic features. Here we report an 11-month-old Caucasian male with global developmental delay, dysmorphic features, coarctation of the aorta, and ventricular septal defect. He was later found to have a pathogenic mutation in the NR2F2 gene by whole exome sequencing. This is the second instance in which an NR2F2 mutation has been identified in a child with a congenital heart defect and other anomalies. This case suggests that some variants in NR2F2 may cause syndromic forms of congenital heart defect. © 2018 Wiley Periodicals, Inc.

  17. Schmidt. Sinfonie Nr. 1 E-Dur; Strauss. Vier sinfonische Zwischenspiele aus Intermezzo. Detroit Symphony Orchestra, Neeme Järvi / Helge Grünewald

    Index Scriptorium Estoniae

    Grünewald, Helge

    1996-01-01

    Uuest heliplaadist "Schmidt. Sinfonie Nr. 1 E-Dur; Strauss. Vier sinfonische Zwischenspiele aus Intermezzo. Detroit Symphony Orchestra, Neeme Järvi. Chandos/Koch CD 9357 (WD: 68'20") DDD (WD:114'36")

  18. Nuclear receptors of the NR4a family are not required for the development and function of follicular T helper cells.

    Science.gov (United States)

    Ma, Weiwei; Zhao, Ruozhu; Yang, Runqing; Liu, Bo; Chen, Xin; Wu, Longyan; Qi, Hai

    2015-10-01

    Follicular T helper (Tfh) cells promote germinal center (GC) reaction and high-affinity antibody production. The molecular mechanisms that regulate development and function of Tfh cells are not fully understood. Here we report that ligand-independent nuclear receptors of the Nr4a family are highly expressed in Tfh cells. In a well-established adoptive transfer model, enforced expression of Nr4a receptors reduces helper T cell expansion but apparently increased the T cell capacity to promote the GC response. On the other hand, deletion of all Nr4a receptors in T cells did not significantly affect expansion or differentiation of Tfh cells or the development of GC reaction. These findings suggest that Nr4a receptors may promote but are not necessary for Tfh development or function in vivo. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. The nuclear hormone receptor family member NR5A2 controls aspects of multipotent progenitor cell formation and acinar differentiation during pancreatic organogenesis.

    Science.gov (United States)

    Hale, Michael A; Swift, Galvin H; Hoang, Chinh Q; Deering, Tye G; Masui, Toshi; Lee, Youn-Kyoung; Xue, Jumin; MacDonald, Raymond J

    2014-08-01

    The orphan nuclear receptor NR5A2 is necessary for the stem-like properties of the epiblast of the pre-gastrulation embryo and for cellular and physiological homeostasis of endoderm-derived organs postnatally. Using conditional gene inactivation, we show that Nr5a2 also plays crucial regulatory roles during organogenesis. During the formation of the pancreas, Nr5a2 is necessary for the expansion of the nascent pancreatic epithelium, for the subsequent formation of the multipotent progenitor cell (MPC) population that gives rise to pre-acinar cells and bipotent cells with ductal and islet endocrine potential, and for the formation and differentiation of acinar cells. At birth, the NR5A2-deficient pancreas has defects in all three epithelial tissues: a partial loss of endocrine cells, a disrupted ductal tree and a >90% deficit of acini. The acinar defects are due to a combination of fewer MPCs, deficient allocation of those MPCs to pre-acinar fate, disruption of acinar morphogenesis and incomplete acinar cell differentiation. NR5A2 controls these developmental processes directly as well as through regulatory interactions with other pancreatic transcriptional regulators, including PTF1A, MYC, GATA4, FOXA2, RBPJL and MIST1 (BHLHA15). In particular, Nr5a2 and Ptf1a establish mutually reinforcing regulatory interactions and collaborate to control developmentally regulated pancreatic genes by binding to shared transcriptional regulatory regions. At the final stage of acinar cell development, the absence of NR5A2 affects the expression of Ptf1a and its acinar specific partner Rbpjl, so that the few acinar cells that form do not complete differentiation. Nr5a2 controls several temporally distinct stages of pancreatic development that involve regulatory mechanisms relevant to pancreatic oncogenesis and the maintenance of the exocrine phenotype. © 2014. Published by The Company of Biologists Ltd.

  20. Avaliação do cumprimento da NR-18 em função do porte de obra residencial e proposta de lista de verificação da NR-18

    Directory of Open Access Journals (Sweden)

    Marcelo Fabiano Costella

    Full Text Available O presente trabalho investiga as reais condições dos ambientes de trabalho da indústria da construção, tendo em vista os problemas relacionados à segurança do trabalho nos canteiros de obras. Formulou-se a hipótese de haver discrepância no grau de cumprimento da NR-18 em obras de diferentes portes (pequena, média e grande. Baseado em uma lista de verificação do grau de cumprimento dos requisitos da NR-18, foram avaliados 115 canteiros de obra na cidade de Chapecó - SC. Para cada item da lista de verificação foi atribuída uma nota e, após compilar as informações, estas foram avaliadas conforme os objetivos da pesquisa. Fez-se uma análise crítica para os cinco piores e os cinco melhores itens observados para as obras pequenas, médias e grandes. Analisando-se os resultados é possível afirmar que há uma diferença significativa de aplicação da NR-18 nos diferentes portes de obras, sendo que as obras de grande porte obtiveram uma média final (6,47 significativamente superior em relação às obras pequenas (1,97. Desta forma, foi corroborada a hipótese formulada, podendo se atribuir este resultado a diversos fatores, entre os quais a falta de habilidades gerenciais de segurança e saúde do trabalho nas pequenas empresas. Outra contribuição deste artigo foi a proposta de uma nova lista de verificação para avaliar o grau de cumprimento da NR-18 nos canteiros de obra.

  1. Leptin reverses corticosterone-induced inhibition of neural stem cell proliferation through activating the NR2B subunits of NMDA receptors

    Energy Technology Data Exchange (ETDEWEB)

    Shi, Wen-Zhu [Anesthesia and Operation Center, Hainan Branch of Chinese PLA General Hospital, Hainan 572013 (China); Anesthesia and Operation Center, Chinese PLA General Hospital, Beijing 100853 (China); Miao, Yu-Liang [Department of Anesthesiology, PLA No. 306 Hospital, Beijing 100101 (China); Guo, Wen-Zhi [Department of Anesthesiology, Beijing Military General Hospital of Chinese People’s Liberation Army, Beijing 100700 (China); Wu, Wei, E-mail: wwzwgk@163.com [Department of Head and Neck Surgery of Otolaryngology, PLA No. 306 Hospital, Beijing 100101 (China); Li, Bao-Wei [Department of Head and Neck Surgery of Otolaryngology, PLA No. 306 Hospital, Beijing 100101 (China); An, Li-Na [Department of Anesthesiology, Armed Police General Hospital, Beijing 100039 (China); Fang, Wei-Wu [Department of Anesthesiology, PLA No. 306 Hospital, Beijing 100101 (China); Mi, Wei-Dong, E-mail: elite2005gg@163.com [Anesthesia and Operation Center, Chinese PLA General Hospital, Beijing 100853 (China)

    2014-04-25

    Highlights: • Leptin promotes the proliferation of neural stem cells isolated from embryonic mouse hippocampus. • Leptin reverses corticosterone-induced inhibition of neural stem cell proliferation. • The effects of leptin are partially mediated by upregulating NR2B subunits. - Abstract: Corticosterone inhibits the proliferation of hippocampal neural stem cells (NSCs). The removal of corticosterone-induced inhibition of NSCs proliferation has been reported to contribute to neural regeneration. Leptin has been shown to regulate brain development, improve angiogenesis, and promote neural regeneration; however, its effects on corticosterone-induced inhibition of NSCs proliferation remain unclear. Here we reported that leptin significantly promoted the proliferation of hippocampal NSCs in a concentration-dependent pattern. Also, leptin efficiently reversed the inhibition of NSCs proliferation induced by corticosterone. Interestingly, pre-treatment with non-specific NMDA antagonist MK-801, specific NR2B antagonist Ro 25-6981, or small interfering RNA (siRNA) targeting NR2B, significantly blocked the effect of leptin on corticosterone-induced inhibition of NSCs proliferation. Furthermore, corticosterone significantly reduced the protein expression of NR2B, whereas pre-treatment with leptin greatly reversed the attenuation of NR2B expression caused by corticosterone in cultured hippocampal NSCs. Our findings demonstrate that leptin reverses the corticosterone-induced inhibition of NSCs proliferation. This process is, at least partially mediated by increased expression of NR2B subunits of NMDA receptors.

  2. NR2E3 mutations in enhanced S-cone sensitivity syndrome (ESCS), Goldmann-Favre syndrome (GFS), clumped pigmentary retinal degeneration (CPRD), and retinitis pigmentosa (RP).

    Science.gov (United States)

    Schorderet, Daniel F; Escher, Pascal

    2009-11-01

    NR2E3, also called photoreceptor-specific nuclear receptor (PNR), is a transcription factor of the nuclear hormone receptor superfamily whose expression is uniquely restricted to photoreceptors. There, its physiological activity is essential for proper rod and cone photoreceptor development and maintenance. Thirty-two different mutations in NR2E3 have been identified in either homozygous or compound heterozygous state in the recessively inherited enhanced S-cone sensitivity syndrome (ESCS), Goldmann-Favre syndrome (GFS), and clumped pigmentary retinal degeneration (CPRD). The clinical phenotype common to all these patients is night blindness, rudimental or absent rod function, and hyperfunction of the "blue" S-cones. A single p.G56R mutation is inherited in a dominant manner and causes retinitis pigmentosa (RP). We have established a new locus-specific database for NR2E3 (www.LOVD.nl/eye), containing all reported mutations, polymorphisms, and unclassified sequence variants, including novel ones. A high proportion of mutations are located in the evolutionarily-conserved DNA-binding domains (DBDs) and ligand-binding domains (LBDs) of NR2E3. Based on homology modeling of these NR2E3 domains, we propose a structural localization of mutated residues. The high variability of clinical phenotypes observed in patients affected by NR2E3-linked retinal degenerations may be caused by different disease mechanisms, including absence of DNA-binding, altered interactions with transcriptional coregulators, and differential activity of modifier genes.

  3. Multigeneration Inheritance through Fertile XX Carriers of an NR0B1 (DAX1 Locus Duplication in a Kindred of Females with Isolated XY Gonadal Dysgenesis

    Directory of Open Access Journals (Sweden)

    Michela Barbaro

    2012-01-01

    Full Text Available A 160 kb minimal common region in Xp21 has been determined as the cause of XY gonadal dysgenesis, if duplicated. The region contains the MAGEB genes and the NR0B1 gene; this is the candidate for gonadal dysgenesis if overexpressed. Most patients present gonadal dysgenesis within a more complex phenotype. However, few independent cases have recently been described presenting with isolated XY gonadal dysgenesis caused by relatively small NR0B1 locus duplications. We have identified another NR0B1 duplication in two sisters with isolated XY gonadal dysgenesis with an X-linked inheritance pattern. We performed X-inactivation studies in three fertile female carriers of three different small NR0B1 locus duplications identified by our group. The carrier mothers did not show obvious skewing of X-chromosome inactivation, suggesting that NR0B1 overexpression does not impair ovarian function. We furthermore emphasize the importance to investigate the NR0B1 locus also in patients with isolated XY gonadal dysgenesis.

  4. Fourth report by the Federal German Government to the Bundestag on measures for the protection of the ozone layer; 4. Bericht der Bundesregierung an den Deutschen Bundestag ueber Massnahmen zum Schutz der Ozonschicht

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-07-01

    This report, which relates the situation as of July 1997, again confirms the internationally leading role of the Federal German Republic in the phase-out of CFCs. No country has realised a more comprehensive concept for the phase-out of substances that deplete the ozone layer. Combining statutory and voluntary measures has proved a path-breaking approach. One of the most important driving forces in the CFC phase-out were the restrictions of use imposed by the Ordinance on the Prohibition of CFCs and Halon. (orig./SR) [Deutsch] Dieser Bericht - mit Stand vom Juli 1997 - bestaetigt erneut die internationale Fuehrungsrolle der Bundesrepublik Deutschland beim FCKW-Ausstieg. In keinem anderen Staaat wurde ein umfassenderes Konzept zum Ausstieg aus den ozonabbauenden Stoffen realisiert. Als wegweisend hat sich dabei die Verknuepfung ordnungsrechtlicher und freiwilliger Massnahem erwiesen. Insbesondere von den Verwendungsbeschraenkungen der FCKW-Halon-Verbots-Verordnung gingen wichtige Impulse beim FCKW-Ausstieg aus. (orig./SR)

  5. Opinion presented on the behalf of the Commission for economic affairs on the finance bill project for 2018 (nr 235). Volume VII - Ecology, sustainable development and mobility, energy - Nr 264 corrected

    International Nuclear Information System (INIS)

    Bolo, Philippe

    2017-01-01

    In its first part, this parliamentary report proposes an analysis of budgets allocated to some specific programmes related to ecology, sustainable development and mobility, and energy within the finance bill project for 2018. A first programme (nr 174) addresses issues related to energy, climate, and after-mines: increased credits for energy, decreased credits to manage the after-mines situation, increased credits for air quality and for the struggle against climate change, tax expenses. A second set of programmes (nr 345, 764 and 765) concerns the energy public service and energy transition. The third set of programmes (nr 793 and 794) deals with the financing of supports to communities for rural electrification. The second part of the report analyses various aspects of the digitalisation of the energy sector. It discusses its main stakes: a better knowledge of productions and consumptions, an increased efficiency of infrastructures and energy-related services, a need to secure infrastructures and data, a high potential for employment and increasing needs for specific abilities, and critical issue of a possible 'uberisation' of the energy sector. It also analyses perspectives and formulates some proposals regarding data security, support to economic actors, and some specific points of attention (to avoid differences between territories, some negative aspects of digitalisation). Contents of commission debates are provided as well as a list of hearings

  6. Biochemical contents of the ovary and hepatopancreas of Uca longisignalis and Uca nr. minaxContenido bioquímico del ovario y hepatopáncreas de Uca longisignalis y Uca nr. minax

    Directory of Open Access Journals (Sweden)

    Barbara E. Hasek

    2006-09-01

    Full Text Available P align=justify>Biochemical composition of ovary and hepatopancreas tissues in wild populations of Uca longisignalis and Uca nr. minax were monitored during the reproductive season. Total lipid (concentration and content, C (carbon, N (nitrogen, and C:N ratios of the ovary and hepatopancreas were quantified over the course of ovarian maturation. Ovary lipid and C concentration varied significantly over the course of ovarian maturation for both species, but there was no relationship between lipid concentration or hepatopancreas content and the stage of ovarian development in females. Hepatopancreatic lipid and C concentration did not differ between sexes of U. nr. minax. Lipid demands of ovarian maturation thus appear to be met in large part by increased dietary intake and not purely by translocating lipid stores from the hepatopancreas. In both Uca longisignalis and U. nr. minax, the color of the hepatopancreas may be used as an indicator of the lipid and C levels of the hepatopancreas. Cadmium-yellow and lemon-yellow hepatopancreas tissues had the highest lipid concentrations. No evidence could be found to demonstrate depletion of lipid or C concentrations in the hepatopancreas concomitant with ovarian maturation.

  7. Evaluation of Two Robot Vision Control Algorithms Developed Based on N-R and EKG Methods for Slender Bar Placement

    Energy Technology Data Exchange (ETDEWEB)

    Son, Jae Kyung; Jang, Wan Shik; Hong, Sung Mun [Gwangju (Korea, Republic of)

    2013-04-15

    Many problems need to be solved before vision systems can actually be applied in industry, such as the precision of the kinematics model of the robot control algorithm based on visual information, active compensation of the camera's focal length and orientation during the movement of the robot, and understanding the mapping of the physical 3-D space into 2-D camera coordinates. An algorithm is proposed to enable robot to move actively even if the relative positions between the camera and the robot is unknown. To solve the correction problem, this study proposes vision system model with six camera parameters. To develop the robot vision control algorithm, the N-R and EKG methods are applied to the vision system model. Finally, the position accuracy and processing time of the two algorithms developed based based on the EKG and the N-R methods are compared experimentally by making the robot perform slender bar placement task.

  8. Evaluation of Two Robot Vision Control Algorithms Developed Based on N-R and EKG Methods for Slender Bar Placement

    International Nuclear Information System (INIS)

    Son, Jae Kyung; Jang, Wan Shik; Hong, Sung Mun

    2013-01-01

    Many problems need to be solved before vision systems can actually be applied in industry, such as the precision of the kinematics model of the robot control algorithm based on visual information, active compensation of the camera's focal length and orientation during the movement of the robot, and understanding the mapping of the physical 3-D space into 2-D camera coordinates. An algorithm is proposed to enable robot to move actively even if the relative positions between the camera and the robot is unknown. To solve the correction problem, this study proposes vision system model with six camera parameters. To develop the robot vision control algorithm, the N-R and EKG methods are applied to the vision system model. Finally, the position accuracy and processing time of the two algorithms developed based based on the EKG and the N-R methods are compared experimentally by making the robot perform slender bar placement task

  9. Eesti NSV Ministrite Nõukogu 15. mai 1989. a. määruse nr. 168 osalise muutmise kohta

    Index Scriptorium Estoniae

    1990-01-01

    Nõukogude valuuta, välisvaluuta ning muude valuuta- ja teiste väärtuste välismaalt ja välismaale sisse- ja väljaveo, saatmise ja ülekandmise määrustiku" kinnitamise kohta. - Lisatud: NSV Liidu MN määrus "Nõukogude valuuta, välisvaluuta ning muude valuuta- ja teiste väärtuste välismaalt ja välismaale sisse- ja väljaveo, saatmise ja ülekandmise määrustikus" muudatuste tegemise kohta (13. dets. 1989 nr. 1108) ; NSVL MN määrus "MSV Liidu sisetarbijaturu kaitse ning Nõukogude valuuta ja tarbekaupade üle NSV Liidu riigipiiri toimetamisel kuritarvituste vältimise abinõude kohta" (7. dets. 1989 nr. 1093)

  10. Effect of electron beam irradiation on the properties of natural rubber (NR)/styrene-butadiene rubber (SBR) blend

    Energy Technology Data Exchange (ETDEWEB)

    Manshaie, R. [Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111 (Iran, Islamic Republic of); Nouri Khorasani, S., E-mail: saied@cc.iut.ac.i [Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111 (Iran, Islamic Republic of); Jahanbani Veshare, S. [Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111 (Iran, Islamic Republic of); Rezaei Abadchi, M. [Department of Polymer Engineering, Amirkabir University of Technology, Tehran (Iran, Islamic Republic of)

    2011-01-15

    In this study, physico-mechanical properties of NR/SBR blends cured by electron beam irradiation and sulfur were compared. The NR/SBR blends were prepared using a two-roll mill. Electron beam irradiations of 100-400 kGy were applied to cure the blends and changes in physico-mechanical properties were studied as a function of irradiation. Also, oil resistance and the effect of thermal ageing on mechanical properties of the blends were investigated. The results show that the irradiated blends have better mechanical properties than those cured by sulfur system. The irradiation cured samples also exhibited better heat stability than the sulfur cured samples. The blend cured by the highest dose shows the lowest swelling and high oil resistance compared with the other samples cured by irradiation.

  11. Sibelius. Lemminkäinen-Legenden op. 22 Nr. 1-4 / Christoph Schlüren

    Index Scriptorium Estoniae

    Schlüren, Christoph

    1997-01-01

    Uuest heliplaadist "Sibelius. Lemminkäinen-Legenden op. 22 Nr. 1-4, Pohjolas Tochter op. 49, Nächtlicher Ritt und Sonnenaufgang op. 55; Göteborger Sinfoniker, Neeme Järvi; DG CD 453 426-2 (WD: 70'37") DDD Võrreldud: Opus 22: Segerstam (Ondine 852-2); op. 22 und 55; Paavo Järvi" (Virgin 545 213-2); op. 49: Segerstam (Chandos 8965)

  12. The NMDAR subunit NR3A interacts with microtubule-associated protein 1S in the brain

    DEFF Research Database (Denmark)

    Eriksson, Maria; Samuelsson, Helena; Samuelsson, Eva-Britt

    2007-01-01

    -proximal part of the NR3A C-terminus. MAP1S belongs to the same family as MAP1A and MAP1B, and was found to be abundant in both postnatal and adult rat brain. In hippocampal neurons the distribution-pattern of MAP1S resembled that of beta-tubulin III, but a fraction of the protein colocalized with synaptic...

  13. Involvement of spinal NR2B-containing NMDA receptors in oxaliplatin-induced mechanical allodynia in rats

    Directory of Open Access Journals (Sweden)

    Yano Takahisa

    2011-01-01

    Full Text Available Abstract Background Oxaliplatin is a platinum-based chemotherapy drug characterized by the development of acute and chronic peripheral neuropathies. The chronic neuropathy is a dose-limiting toxicity. We previously reported that repeated administration of oxaliplatin induced cold hyperalgesia in the early phase and mechanical allodynia in the late phase in rats. In the present study, we investigated the involvement of NR2B-containing N-methyl-D-aspartate (NMDA receptors in oxaliplatin-induced mechanical allodynia in rats. Results Repeated administration of oxaliplatin (4 mg/kg, i.p., twice a week caused mechanical allodynia in the fourth week, which was reversed by intrathecal injection of MK-801 (10 nmol and memantine (1 μmol, NMDA receptor antagonists. Similarly, selective NR2B antagonists Ro25-6981 (300 nmol, i.t. and ifenprodil (50 mg/kg, p.o. significantly attenuated the oxaliplatin-induced pain behavior. In addition, the expression of NR2B protein and mRNA in the rat spinal cord was increased by oxaliplatin on Day 25 (late phase but not on Day 5 (early phase. Moreover, we examined the involvement of nitric oxide synthase (NOS as a downstream target of NMDA receptor. L-NAME, a non-selective NOS inhibitor, and 7-nitroindazole, a neuronal NOS (nNOS inhibitor, significantly suppressed the oxaliplatin-induced pain behavior. The intensity of NADPH diaphorase staining, a histochemical marker for NOS, in the superficial layer of spinal dorsal horn was obviously increased by oxaliplatin, and this increased intensity was reversed by intrathecal injection of Ro25-6981. Conclusion These results indicated that spinal NR2B-containing NMDA receptors are involved in the oxaliplatin-induced mechanical allodynia.

  14. PRE-DISCOVERY AND FOLLOW-UP OBSERVATIONS OF THE NEARBY SN 2009nr: IMPLICATIONS FOR PROMPT TYPE Ia SUPERNOVAE

    International Nuclear Information System (INIS)

    Khan, Rubab; Stanek, K. Z.; Beacom, J. F.; Szczygiel, D. M.; Mogren, K.; Eastman, J. D.; Martini, P.; Stoll, R.; Prieto, J. L.; Pojmanski, G.; Pilecki, B.

    2011-01-01

    We present photometric and spectroscopic observations of the Type Ia supernova SN 2009nr in UGC 8255 (z = 0.0122). Following the discovery announcement at what turned out to be 10 days after peak, we detected it at V ≅15.7 mag in data collected by the All-Sky Automated Survey (ASAS) North telescope 2 weeks prior to the peak, and then followed it up with telescopes ranging in aperture from 10 cm to 6.5 m. Using early photometric data available only from ASAS, we find that the supernova is similar to the overluminous Type Ia SN 1991T, with a peak at M V ≅ -19.6 mag, and a slow decline rate of Δm 15 (B) ≅ 0.95 mag. The early post-maximum spectra closely resemble those of SN 1991T, while the late-time spectra are more similar to those of normal Type Ia supernovae (SNe Ia). Interestingly, SN 2009nr has a projected distance of 13.0 kpc (∼4.3 disk scale lengths) from the nucleus of the small star-forming host galaxy UGC 8255. This indicates that the progenitor of SN 2009nr is not associated with a young stellar population, calling into question the conventional association of luminous SNe Ia with the 'prompt' component directly correlated with current star formation. The pre-discovery observation of SN 2009nr using ASAS demonstrates the science utility of high-cadence all sky surveys conducted using small telescopes for the discovery of nearby (d ∼< 50 Mpc) supernovae.

  15. NR2B antagonist CP-101,606 abolishes pitch-mediated deviance detection in awake rats

    Directory of Open Access Journals (Sweden)

    Siva eDigavalli

    2014-08-01

    Full Text Available Schizophrenia patients exhibit a decreased ability to detect change in their auditory environment as measured by auditory event related potentials such as mismatch negativity. This deficit has been linked to abnormal NMDA neurotransmission since, among other observations, non-selective channel blockers of NMDA reliably diminish deviance detection in human subjects as well as in animal models. Recent molecular and functional evidence link NR2B receptor subtype to aberrant NMDA transmission in schizophrenia. However, it is unknown if NR2B receptors participate in pre-attentive deviance detection. We recorded event related potentials from the vertex of freely behaving rats in response to frequency mismatch protocols. We saw a robust increase in N1 response to deviants compared to standard as well as control stimuli indicating true deviance detection. Moreover, the increased negativity was highly sensitive to deviant probability. Next, we tested the effect of a non-selective NMDA channel blocker (ketamine, 30 mg/kg and a highly selective NR2B antagonist, CP-101,606 (10 or 30 mg/kg on deviance detection. Ketamine attenuated deviance mainly by increasing the amplitude of the standard ERP. Amplitude and/or latency of several ERP components were also markedly affected. In contrast, CP-101,606 robustly and dose-dependently inhibited the deviant’s N1 amplitude and as a consequence, completely abolished deviance detection. No other ERPs or components were affected. Thus, we report first evidence that NR2B receptors robustly participate in processes of automatic deviance detection in a rodent model. Lastly, our model demonstrates a path forward to test specific pharmacological hypotheses using translational endpoints relevant to aberrant sensory processing in schizophrenia.

  16. Examination of the somatostatin receptor status in non-medullary thyroid cancer; Untersuchungen zum Somatostatinrezeptor-Status bei nicht-medullaeren Schilddruesenkarzinomen

    Energy Technology Data Exchange (ETDEWEB)

    Goerges, R.; Brandt-Mainz, K.; Bockisch, A. [Essen Univ. (Gesamthochschule) (Germany). Klinik und Poliklinik fuer Nuklearmedizin; Kahaly, G. [Mainz Univ. (Germany). Klinik und Poliklinik fuer Medizin - Endokrinologie und Stoffwechselerkrankungen; Mueller-Brand, J.; Maecke, H. [Kantonsspital Basel (Switzerland). Inst. fuer Nuklearmedizin; Walgenbach, S. [Mainz Univ. (Germany). Klinik und Poliklinik fuer Allgemein- und Abdominalchirurgie; Bruns, C. [Praeklinische Forschung Novartis, Basel (Switzerland); Andreas, J. [Universitaetsklinik Mainz (Germany). Klinik und Poliklinik fuer Nuklearmedizin

    1999-06-01

    -medullaeren Schilddruesenkarzinomen nachgewiesen. In der vorliegenden Studie wird der Somatostatinrezeptor-Status bei dieser Tumorentitaet in einem groesseren Patientenkollektiv untersucht. Patienten und Methoden: Wir vergleichen 131-Iod- mit 111-In-Pentetreotide-Szintigraphien bei 24 Patienten mit metastasiertem, nicht-medullaeren Schilddruesenkarzinom. Die Befunde wurden mit anderen bildgebenden Verfahren korreliert. Zusaetzlich erfolgten Rezeptor-Autoradiographien bei einem Patienten, eine Octreotide-Behandlung bei einem anderen und die Verabreichung von 90-Y- sowie 111-In-DOTATOC bei zwei weiteren Patienten. Ergebnisse: Bei 8/15 Patienten mit papillaerem oder follikulaerem Schilddruesenkarzinom erwies sich 111-In-Pentetreotide dem 131-I- als unterlegen, bei 1/15 als gleichwertig und bei 6/15 als ueberlegen. Bei 8/9 Patienten mit onkozytaerem Schilddruesenkarzinom zeigten die Metastasen eine 111-In-Pentetreotide-Akkumulation unterschiedlicher Intensitaet, waehrend die 131-I-Szintigraphien hier bis auf eine Ausnahme negativ waren. Im Vergleich mit 201-Tl oder 99m-Tc-Sestamibi erwies sich 111-In-Pentetreotide als gleichwertig oder ueberlegen, aber im Vergleich zum 18-F-FDG-PET zumeist als unterlegen. Die Befunde der 111-In-Pentetreotide-Szintigraphie korrelierten gut mit der Rezeptor-Autoradiographie und der DOTATOC-Akkumulation, nicht dagegen mit dem Behandlungseffekt von `kaltem` Octreotide auf die Schilddruesenkarzinom-Metastasen. Schlussfolgerungen: Diverse Metastasen papillaerer und follikulaerer Schilddruesenkarzinome und die Mehrzahl der onkozytaeren Schilddruesenkarzinom-Metastasen koennen Somatostatin-Rezeptoren exprimieren. Insbesondere beim onkozytaeren Schilddruesenkarzinom beziehungsweise bei nicht verfuegbarem PET erweist sich die 111-In-Pentetreotide-Szintigraphie als vielversprechende Methode zur Metastasenlokalisation; sie konnte ferner von Nutzen sein bei der Selektion moeglicher Kandidaten fuer eine Therapie mit {beta}-Strahler-markierten Somatostatinanaloga, wenn diese fuer die

  17. Neurocognition and PET: strategies for data analysis in activation studies on working memory; Neurokognition und PET: datenanalytische Strategien bei Aktivierungsstudien zum Arbeitsgedaechtnis

    Energy Technology Data Exchange (ETDEWEB)

    Hautzel, H.; Mottaghy, F.M.; Schmidt, D.; Mueller, H.-W.; Krause, B. J. [Klinik fuer Nuklearmedizin (KME), Forschungszentrum Juelich (Germany); Nuklearmedizinische Klinik, Heinrich-Heine-Universitaet Duesseldorf (Germany)

    2003-10-01

    Verwendung eines verbalen n-zurueck-Arbeitsgedaechtnis-Paradigmas (n von 0 bis 3) werden kognitive Subtraktionsanalysen als Basisauswertung, parametrische und Kovarianz-basierte Analysen vorgestellt. Letztere wurden unter Anwendung einer externen Kovariante (individuelle Rate der korrekten Antworten), mittels regionaler Interkorrelationen und als Netzwerkanalyse mit Hilfe struktureller Gleichungssysteme zur Modellierung der effektiven Konnektivitaet zwischen den beteiligten Hirnregionen durchgefuehrt. Ergebnisse: Die Subtraktionsanalysen stellten eine zunehmende Involvierung eines fronto-parientalen Netzwerkes unter Einschluss des anterioren cingulaeren Kortex und Kleinhirnanteilen dar. Die parametrische Analyse wie einen linear mit der Aufgabenschwierigkeit ansteigenden rCBF beidseits praefrontal, inferior parietal unter Einschluss des Praecuneus, im prae-supplementaer-motorischen Kortex links und im anterior cingulaeren Kortex nach. Die Korrelationsanalyse mit Nutzung einer externen Kovarianten demonstrierte von dieser negativ abhaengige rCBF-Veraenderungen beidseits inferior parietal und im Praecuneus, bilateral praefrontal und im anterior cingulaeren Kortex. In der Netzwerkanalyse zur Beschreibung der effektiven Konnektivitaet wurden vorwiegend von okzipital nach frontal gerichtete, links-hemisphaerisch dominante Interaktionen nachgewiesen. Schlussfolgerung: Die vorgestellten datenanalytischen Vorgehensweisen tragen in ihrer Gesamtheit zum umfassenden Verstaendnis kognitiver Prozesse und einzelner Teilaspekte bei. (orig.)

  18. Curcumin attenuates the expression of NMDAR-NR1 in Chronic Constructive Injury model of neuropathic pain

    Directory of Open Access Journals (Sweden)

    Xiangdi Yu

    2015-03-01

    Full Text Available Objective: Neuropathic pain is a prevalent desease that greatly impairs the patients’ quality of life. A lack of the understanding of its aetiology, inadequate relief, and development of tolerance and potential toxicity of classical antinociceptives warrant the investigation of the newer agents to relieve this pain. The aim of the present study was to explore the antinociceptic effect of curcumin and its effect on expression of N-methyl-D-aspartate (NMDA receptor in spinal dorsal horn and dorsal root ganglion in chronic constriction injury (CCI mode of neuropathic pain of rats. Methods: Paw withdrawal mechanical threshold (PWMT and paw withdrawal thermal latency (PWTL of rats were measured on 2th pre-operative and 1, 3, 5, 7, 10, 14 post-operative days, and the expression of NMDAR NR-1 in spinal dorsal horn and DRG was measured by Immunohistochemical staining and western-blot. Results: CCI rats exhibited significant hyperalgesia after operation as compared with control rats. Chronic treatment with curcumin 100mg/kg/day for 14days starting from the 1 th day after CCI operation significantly attenuated PWMT and PWTL. Curcumin also inhibited the expression of NMDAR NR-1 in spinal dorsal horn and DRG. Conclusion: These results indicate an antinociceptive activity of curcumin possibly through its inhibitory action on expression of NMDAR NR-1 in spinal dorsal horn and DRG and point towards its potential to attenuate neuropathic pain.

  19. Effective closed form mathematical approach to determine kinetic constants of NR vulcanized with sulphur and accelerators at different concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Milani, Gabriele, E-mail: milani@stru.polimi.it, E-mail: gabriele.milani@polimi.it [Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan (Italy); Hanel, Thomas; Donetti, Raffaella [Pirelli Tyre, Via Alberto e Piero Pirelli 25, 20126 Milan (Italy); Milani, Federico [CHEMCO Consultant, Via J.F. Kennedy 2, 45030 Occhiobello (Italy)

    2015-03-10

    The basic reaction scheme due to Han and co-workers for NR vulcanized with sulphur is adopted and modified taking into account the single contributions of the different accelerators, focusing in particular on some experimental data ad hoc obtained at Pirelli’s laboratories, where NR was vulcanized at different temperatures (from 150 to 180 °C) and concentrations of sulphur, using TBBS and DPG in the mixture as co-agents. Typically, the chain reactions are initiated by the formation of macro-compounds that are responsible of the formation of the unmatured crosslinked polymer. This first reaction depends on the reciprocal concentrations of all components and their chemical nature. In presence of two accelerators, it was considered that the reactions between each single accelerator and the NR raw material occur in parallel, making the reasonable assumption that there are no mutual reactions between the two accelerators. From the kinetic scheme adopted, a closed form solution was found for the crosslink density, with the only limitation that the induction period is excluded from computations. Even kinetic constants are evaluated in closed form, avoiding a numerically demanding least-squares best fitting on rheometer experimental data. Two series of experiments available, relying into rheometer curves at different temperatures and different concentrations of sulphur and accelerator, are utilized to evaluate the fitting capabilities of the mathematical model. Very good agreement between numerical output and experimental data is experienced in all cases analysed.

  20. Efeito da ordem de adição de componentes nas propriedades de misturas NR/BR Properties of NR/BR mixtures and their dependence on the preparation mode

    Directory of Open Access Journals (Sweden)

    Daniele F. Castro

    2007-03-01

    Full Text Available Misturas de elastômeros vêm sendo largamente utilizadas na indústria de borracha com a finalidade de se conseguir propriedades específicas, sem a necessidade de sintetizar novos polímeros. O polibutadieno puro (BR não apresenta boas características de processamento e de resistência à tração e ao rasgamento sendo, por essa razão, normalmente utilizado em misturas com outros elastômeros, principalmente NR e SBR. Tais misturas são amplamente utilizadas na indústria de pneus. Neste trabalho o polibutadieno (BR foi misturado à borracha natural (NR na proporção de 1:1 em peso. As composições foram preparadas em misturador de cilindros, empregando-se quatro maneiras distintas de incorporação dos aditivos. Após a vulcanização, foram estudadas as propriedades mecânicas (resistência à tração, ao rasgamento e dureza e a morfologia das composições. Os resultados mostram que, apesar de apresentarem a mesma formulação, as propriedades das misturas são bastante influenciadas pelo modo de preparo.Elastomer Blends have been widely used in the rubber industry with the purpose of reaching specific properties without the need of synthesizing new polymers. Polybutadiene rubber (BR presents poor stress and tear strengths, which is the main reason why BR is usually blended with NR and SBR. These blends are extensively used in the manufacture of tyres. In this work natural rubber (NR was mixed to polybutadiene rubber (BR in a 50:50 w/w ratio. The compositions were prepared in a two-roll mill employing four different modes of introducing the additives. After vulcanization, the mixtures were studied with evaluation of their mechanical (stress and tear strengths and hardness properties and morphology. The results show that the properties varied even using the same NR/BR formulations due to the different preparation techniques.

  1. Misturas NR/SBR: modos de preparação e propriedades

    Directory of Open Access Journals (Sweden)

    Visconte Leila L. Y.

    2001-01-01

    Full Text Available Aplicações de artefatos de borracha muitas vezes requerem um conjunto de propriedades que não podem ser fornecidas por um único elastômero sendo, então necessário, que misturas de dois ou mais polímeros sejam empregadas. Nesses casos, os demais ingredientes que normalmente são adicionados, distribuem-se de modo diferente dependendo de sua afinidade com cada uma das borrachas. O grau de dispersão de cada um desses ingredientes em cada fase elastomérica irá influenciar a velocidade e o grau de vulcanização e, certamente, terá conseqüências sobre o desempenho do composto final. Neste trabalho, a borracha natural (NR foi misturada ao elastômero de butadieno-estireno (SBR na proporção de 1:1 em peso. As composições foram preparadas em misturador de cilindros, segundo a norma ASTM D 3182, empregando-se quatro maneiras distintas para a incorporação dos aditivos. Após a vulcanização foram estudadas propriedades mecânicas, como resistências à tração e ao rasgamento e dureza, propriedades termo-dinâmico-mecânicas e a morfologia de cada uma das composições. As propriedades mecânicas foram avaliadas de acordo com as normas ASTM específicas para cada ensaio. Os resultados mostram que, apesar de se usar a mesma formulação, o modo de preparo tem grande influência sobre as propriedades, o que é detectável pelas propriedades mecânicas e pela análise morfológica, e evidenciado através de testes sensíveis, como o dinâmico-mecânico. No caso da mistura NR/SBR as melhores propriedades são obtidas quando é favorecida a vulcanização da borracha que, isoladamente, apresenta propriedades inferiores.

  2. The NR-radon intensity impact on floral environment around NAPP

    International Nuclear Information System (INIS)

    Maury, A.N.; Sharma, M.; Singh, B.; Kumar, R.; Swarup, R.

    2006-01-01

    The nuclear radiation level around Narora Atomic Power Plant (NAPP), District Buladshehar (Uttar Pradesh) has been observed by experimental irradiation of CR-39 plastic track detectors, mounted in a self assembled box installed at height from the ground level 10, 20, 40, 60, 80 and 100 cm. The plants being most sensitive to nuclear radiation, constituting the floral component of bio-diversity, look more frequent and effective means to regulate the nuclear radiation and hence employed as potential alternative to SSNTD, named as BSNTD (the Bio-Species-Nuclear Track Detector) after appropriate calibrations. The five sites selected with similar settings of NR experimental 45 days exposures for Radon alpha, also accompanied by vegetable plants, oil yielding and ornamental plants such as radish, onion, mustard and marigold (under various physical as well as chemical treatments such as magnetic fields, fertilizers) placed at: (1)The open space nearly ∼ 100 meter away from NAPP Reactor Unit No. 1, (2) Village Ratanpur about 10 Km from NAPP, (3) Village Shehdol near Atrauli about 20 Km from NAPP, (4) Kasimpur Thermal power Plant near Aligarh, about 40 Km from NAPP and (5) The D.S. College, Physics research laboratory at Aligarh proper. The isolated and mixed treated plants were grown under controlled favourable conditions and their regular biological growth like total shoot yield, flowers/fruits and seed yield were recorded and these were correlated with the observed radioactive data. The experimental findings seem to match with all these parameters proportionally to CR-39 recording and thus proposing much cheaper Bio-Species Nuclear Track Detection as dynamical physiological changes but not as rigid geometrical damages recorded in SSNTD under cumbersome experimental procedures. The plant growth performance under different environmental conditions such as presence of track elements and heavy metals, our research group had set an unique experimentation relating complex bio

  3. Verfahren zum Elektrodialysieren von Fluessigkeiten

    OpenAIRE

    Boergardts, P.

    1998-01-01

    This new method is used for the electrodialysis of fluids, accompanied by water electrolysis. Fluid containing a salt undergoes monopolar electrodialysis. Concentrate flowing from this stage is then subjected to bipolar electrodialysis. A novel feature of this process is that the concentrate stream undergoing bipolar electrodialysis, is mixed with fluid which has been reduced in concentration by bipolar electrodialysis, i.e. resultant dilutate. Also claimed is the equipment to carry out the p...

  4. Zum Interview mit Arthur Schnitzler

    DEFF Research Database (Denmark)

    Pinkert, Ernst-Ullrich

    2015-01-01

    Kommentar til et interview med Schnitzler, som dagbladet Politiken publicerede i 1923 og som E.U.Pinkert oversatte til tysk. Oversættelsen udkom den 28.11.2015 i Frankfurter Allgemeine Zeitung med titlen "Eine Gefahr für die Jugend?"...

  5. Vom Nachhaltigkeitsdiskurs zum hochschuldidaktischen Kriteriensatz

    Directory of Open Access Journals (Sweden)

    Ulrike Greb

    2009-08-01

    Full Text Available Als regulative Idee impliziert die „Nachhaltigkeitsidee“ eine Reihe geisteswissenschaftlicher Theoreme. Sie gilt daher als überkomplex und konnte bislang hochschuldidaktisch nicht systematisch transformiert wurden. Zur Rede stehen hier begriffliche Großprojekte wie Gerechtigkeit, Verantwortung, Partizipation, Retinität oder Globalität und Interkulturalität, die als Kristallisationspunkte der Nachhaltigkeitsidee die Grundlagentexte, Positionspapiere und Programme durchziehen. Im Anschluss an die heuristische Studie Globalität und Interkulturalität als integrale Bestandteile beruflicher Bildung für eine nachhaltige Entwicklung (GInE, www.ibw.uni-hamburg.de/GInE wurde ein didaktisches Strukturgitter entworfen, das exemplarische Problemlagen und Dilemmata benennt, so wie Reflexionskategorien für die Hochschuldidaktik in der beruflichen Lehrerbildung zur Diskussion stellt.As a regulative concept, the idea of sustainability implies a number of theorems in the field of humanities. It is regarded as supercomplex and, thus, could not yet be systematically transformed in university didactics. Large scale conceptual terms such as justice, responsibility, participation, retinity, globality, and interculturality are at the core of the discussion – terms which run through basic texts, policy papers, and programmes as focal points of the idea of sustainability. With respect to the heuristic study Globality and Interculturality as Integral Elements of a Professional Education for a Sustainable Development (GInE, www.ibw.uni-hamburg.de/GInE, a didactic framework was designed which not only denotes exemplary problems and dilemmata but also puts up categories of reflection for a university didactics aiming at a professional teacher training.

  6. Sammelrezension: Neue Publikationen zum Horrorfilm

    OpenAIRE

    Stiasny, Philipp

    2005-01-01

    Reynold Humphries: The American Horror Film. An IntroductionPeter Hutchings: The Horror FilmJoseph Maddrey: Nightmares in Red, White and Blue. The Evolution of the American Horror FilmBarry Keith Grant, Christopher Sharret (Hg.): Planks of Reason. Essays on the Horror Film

  7. Ingo Schwarz zum 65. Geburtstag

    Directory of Open Access Journals (Sweden)

    Die Mitarbeiterinnen und Mitarbeiter der Alexander-von-Humboldt-Forschungsstelle

    2014-12-01

    Full Text Available Am 17. Dezember 2014 feiert der langjährige Arbeitsstellenleiter der Alexander-von-Humboldt-Forschungsstelle der Berlin-Brandenburgischen Akademie der Wissenschaften Ingo Schwarz seinen 65. Geburtstag. Aus diesem Anlass haben Freunde, Kollegen und Wegbegleiter Beiträge zusammengetragen, die die Bandbreite der jahrzehntelangen Forschungen Ingo Schwarz’ zu Alexander von Humboldt widerspiegeln.

  8. Zum Typus des ossetischen Kasussystems

    Directory of Open Access Journals (Sweden)

    Karl Horst Schmidt

    1993-12-01

    Full Text Available Altiranisch ist das altindogermanische Kasussystem weitgehend erhalten geblieben; im Singular (Sg. der avestischen (av. Nominaldeklination werden noch die acht Kasus Nominativ, Akkusativ, Genetiv, Dativ, Ablativ, Lokativ, Instrumenta! und Vokativ unterschieden; z.B. o-Stamm ahura- 'Gott': ahuri5, ahuram, ahurahyii., ahurai usw.; im Altpersischen ist dieses System durch den Zusammenfall von Dativ und Genetiv um einen Kasus reduziert worden. Auch in der typologischen Anordnung der Morpheme hat das Altiranische den aus dem Indogermanischen (idg. ererbten Status einer flektierenden Sprache bewahrt. Es ist charakterisiert durch Merkmale wie die Differenzierung von rrwnothematischer und heteroklitischer Deklination2 (vgl. Stamm vs. r/n-Stamm: av. ahura- vs. hvarǝ 'Sonne', Genetiv xvǝng, Caland-Wackernagelschen Suffixwechsel (av. dǝrǝz-ra- 'fest' : dǝrǝz-i-ra0a- 'festen Wagen habend' 3, Ablaut (av. dātā 'Geber', Genetiv dāvro, Formvariation4 (aav. Genetiv Sg. ahura-hyā vs. dāvr-ō oder Autonomie des Wortes.

  9. Albert Keding zum 65. Geburtstag

    OpenAIRE

    Pietsch, Torsten

    2014-01-01

    Herr Albert Keding beging am 2. März 1997 seinen 65. Geburtstag. Er wurde in Schreitlacken, Kreis Samland (Ostpreußen) geboren, wo seine Eltern einen Landwirtschaftsbetrieb besaßen. Dort wurde das Interesse des Jubilars zur Landwirtschaft geweckt. So besuchte Albert Keding später in Dassow-Lütgenhof (Mecklenburg) eine Fachschule für Landwirtschaft, wo er seine Vorliebe für Gräser entdeckte. An diese Ausbildung schloss sich ein Landwirtschaftsstudium an der Martin-Luther-Universität in Halle a...

  10. VERFAHREN ZUM VERBINDEN METALLISCHER SCHAUMWERKSTOFFE

    OpenAIRE

    Gruetzner, H.; Horn, H.

    2003-01-01

    WO 200218089 A UPAB: 20020528 NOVELTY - Joining a first object (1) made of a metallic foam material to a second metallic object (2) in a connecting region (7) comprises moving a rotating friction stud (6) between the objects in the connecting region in a friction fit so that the objects are heated and plasticized as a result of friction and pressure exerted on the objects by the stud and then welding the two objects together. DETAILED DESCRIPTION - Preferred Features: The friction stud is rot...

  11. Estradiol-induced increase in novel object recognition requires hippocampal NR2B-containing NMDA receptors.

    Science.gov (United States)

    Vedder, Lindsey C; Smith, Caroline C; Flannigan, Alaina E; McMahon, Lori L

    2013-01-01

    17β-estradiol (E2), at high circulating levels, enhances learning and memory in many women, making it a clinical treatment for hormone-related cognitive decline in aging. However, the mechanisms stimulated by E2, which are responsible for its cognitive enhancing effects, remain incompletely defined. Using an ovariectomized rat model, we previously reported that increasing plasma E2 enhances the magnitude of long-term potentiation (LTP) at hippocampal CA3-CA1 synapses, which is caused by a selective increase in current mediated by NR2B-containing NMDARs, leading to an increase in the NMDAR/AMPAR ratio. Whether the increase in NR2B current is causally related to the ability of E2 to enhance hippocampal dependent learning and memory has yet to be tested. Here, we find that E2 enhances performance in the novel object recognition (NOR) task with the same time course we previously showed E2 enhances the LTP magnitude, temporally linking the increase in LTP to enhanced learning and memory. Furthermore, using the selective NR2B subunit antagonist Ro25-6981, we find that the E2-enhanced NOR, like the enhanced LTP, requires hippocampal NR2B-containing NMDARs, specifically in area CA1. Finally, using whole-cell recordings and the phosphatase inhibitor orthovanadate, we investigated whether the E2-induced increase in NMDAR current is caused by an increase in the density of synaptic NMDARs and/or an increase in NMDAR subunit phosphorylation. We find that both mechanisms are responsible for the enhanced NMDAR current in E2-treated rats. Our results show that the E2-enhanced NOR requires a functional increase in NR2B-containing NMDARs, a requirement shared with the E2-enhanced LTP magnitude at CA3-CA1 synapses, supporting the hypothesis that the increase in LTP likely contributes to the enhanced learning and memory following an increase in plasma E2 levels. Copyright © 2012 Wiley Periodicals, Inc.

  12. Antagonism at the NR2B subunit of NMDA receptors induces increased connectivity of the prefrontal and subcortical regions regulating reward behavior.

    Science.gov (United States)

    Gass, Natalia; Becker, Robert; Sack, Markus; Schwarz, Adam J; Reinwald, Jonathan; Cosa-Linan, Alejandro; Zheng, Lei; von Hohenberg, Christian Clemm; Inta, Dragos; Meyer-Lindenberg, Andreas; Weber-Fahr, Wolfgang; Gass, Peter; Sartorius, Alexander

    2018-04-01

    Evidence indicates that ketamine's rapid antidepressant efficacy likely results from its antagonism of NR2B-subunit-containing NMDA receptors (NMDAR). Since ketamine equally blocks NR2A- and NR2B-containing NMDAR, and has affinity to other receptors, NR2B-selective drugs might have improved therapeutic efficiency and side effect profile. We aimed to compare the effects of (S)-ketamine and two different types of NR2B-selective antagonists on functional brain networks in rats, in order to find common circuits, where their effects intersect, and that might explain their antidepressant action. The experimental design comprised four parallel groups of rats (N = 37), each receiving (S)-Ketamine, CP-101,606, Ro 25-6981 or saline. After compound injection, we acquired resting-state functional magnetic resonance imaging time series. We used graph theoretical approach to calculate brain network properties. Ketamine and CP-101,606 diminished the global clustering coefficient and small-worldness index. At the nodal level, all compounds induced increased connectivity of the regions mediating reward and cognitive aspects of emotional processing, such as ventromedial prefrontal cortex, septal nuclei, and nucleus accumbens. The dorsal hippocampus and regions involved in sensory processing and aversion, such as superior and inferior colliculi, exhibited an opposite effect. The effects common to ketamine and NR2B-selective compounds were localized to the same brain regions as those reported in depression, but in the opposite direction. The upregulation of the reward circuitry might partially underlie the antidepressant and anti-anhedonic effects of the antagonists and could potentially serve as a translational imaging phenotype for testing putative antidepressants, especially those targeting the NR2B receptor subtype.

  13. Molecular Interactions between NR4A Orphan Nuclear Receptors and NF-κB Are Required for Appropriate Inflammatory Responses and Immune Cell Homeostasis.

    Science.gov (United States)

    Murphy, Evelyn P; Crean, Daniel

    2015-06-29

    Appropriate innate and adaptive immune responses are essential for protection and resolution against chemical, physical or biological insults. Immune cell polarization is fundamental in orchestrating distinct phases of inflammation, specifically acute phase responses followed by resolution and tissue repair. Dysregulation of immune cell and inflammatory responses is a hallmark of multiple diseases encompassing atherosclerosis, rheumatoid arthritis, psoriasis and metabolic syndromes. A master transcriptional mediator of diverse inflammatory signaling and immune cell function is NF-κB, and altered control of this key regulator can lead to an effective switch from acute to chronic inflammatory responses. Members of the nuclear receptor (NR) superfamily of ligand-dependent transcription factors crosstalk with NF-κB to regulate immune cell function(s). Within the NR superfamily the NR4A1-3 orphan receptors have emerged as important regulators of immune cell polarization and NF-κB signaling. NR4A receptors modulate NF-κB activity in a dynamic fashion, either repressing or enhancing target gene expression leading to altered inflammatory outcome. Here we will discuss the pivotal role NR4A's receptors play in orchestrating immune cell homeostasis through molecular crosstalk with NF-κB. Specifically, we will examine such NR4A/NF-κB interactions within the context of distinct cell phenotypes, including monocyte, macrophage, T cells, endothelial, and mesenchymal cells, which play a role in inflammation-associated disease. Finally, we review the therapeutic potential of altering NR4A/NF-κB interactions to limit hyper-inflammatory responses in vivo.

  14. The effect of NMDA-NR2B receptor subunit over-expression on olfactory memory task performance in the mouse.

    Science.gov (United States)

    White, Theresa L; Youngentob, Steven L

    2004-09-17

    The N-methyl-D-aspartate (NMDA) receptor in the forebrain is thought to modulate some forms of memory formation, with the NR2B subunit being particularly relevant to this process. Relative to wild-type mice, transgenic animals in which the NR2B subunit was over-expressed demonstrate superior memory in a number of behavioral tasks, including object recognition [Nature 401 (1999) 63]. The purpose of the present study was to explore the generality of such phenomena, interpreted as the effect of increasing NR2B expression on the retention of other types of sensory-related information. To accomplish this, we focused our evaluation on the highly salient sensory modality of olfaction. In the first experiment, mice performed both a novel-object-recognition task identical to that performed by Tang et al. [Nature 401 (1999) 63] and a novel-odor-recognition task analogously constructed. Although the results of the object recognition task were consistent with the previous literature, there was no evidence of an effect of NR2B over-expression on the retention of odor recognition memory in the specific task performed. As it was possible that, unlike object recognition memory, novel odor recognition is not NMDA-receptor-dependent, a second task was designed using the social transmission of food preference paradigm. In contrast to the foregoing olfactory task, there is evidence that the latter procedure is, indeed, NMDA-dependent. The results of the second study demonstrated that transgenic mice with NR2B over-expression had a clear memory advantage in this alternative odor memory paradigm. Taken together, these results suggest the NR2B subunit is an important component in some but not all forms of olfactory memory organization. Moreover, for those functions that are NMDA-receptor-dependent, these data support the growing literature demonstrating the importance of the NR2B subunit.

  15. Vorinostat, a histone deacetylase inhibitor, facilitates fear extinction and enhances expression of the hippocampal NR2B-containing NMDA receptor gene.

    Science.gov (United States)

    Fujita, Yosuke; Morinobu, Shigeru; Takei, Shiro; Fuchikami, Manabu; Matsumoto, Tomoya; Yamamoto, Shigeto; Yamawaki, Shigeto

    2012-05-01

    Histone acetylation, which alters the compact chromatin structure and changes the accessibility of DNA to regulatory proteins, is emerging as a fundamental mechanism for regulating gene expression. Histone deacetylase (HDAC) inhibitors increase histone acetylation and enhance fear extinction. In this study, we examined whether vorinostat, an HDAC inhibitor, facilitates fear extinction, using a contextual fear conditioning (FC) paradigm, in Sprague-Dawley rats. We found that vorinostat facilitated fear extinction. Next, the levels of global acetylated histone H3 and H4 were measured by Western blotting. We also assessed the effect of vorinostat on the hippocampal levels of NMDA receptor mRNA by real-time quantitative PCR (RT-PCR) and protein by Western blotting. 2 h after vorinostat administration, the levels acetylated histones and NR2B mRNA, but not NR1 or NR2A mRNA, were elevated in the hippocampus. The NR2B protein level was elevated 4 h after vorinostat administration. Last, we investigated the levels of acetylated histones and phospho-CREB (p-CREB) binding at the promoter of the NR2B gene using the chromatin immunoprecipitation (ChIP) assay followed by RT-PCR. The ChIP assay revealed increases in the levels of acetylated histones and they were accompanied by enhanced binding of p-CREB to its binding site at the promoter of the NR2B gene 2 h after vorinostat administration. These findings suggest that vorinostat increases the expression of NR2B in the hippocampus by enhancing histone acetylation, and this process may be implicated in fear extinction. Copyright © 2012 Elsevier Ltd. All rights reserved.

  16. Limited field investigation report for the 100-NR-1 operable unit. Draft A

    International Nuclear Information System (INIS)

    1994-08-01

    This limited field investigation (LFI) report summarizes the data collection and analysis activities conducted during the 100-NR-1 Source Operable Unit LFI and the associated qualitative risk assessment (QRA), and makes recommendations on the continued candidacy of high-priority sites for interim remedial measures (IRM). The results and recommendations presented in this report are generally independent of future land-use scenarios. An LFI report is required when waste sites are to be considered for IRM and existing data are insufficient to formulate a conceptual model or perform a QRA. The purpose of the report is to identify those sites that are recommended to remain as candidates for IRM, provide a preliminary summary of site characterization studies, refine the conceptual model as needed, identify potential contaminant and location-specific applicable or relevant and appropriate requirements (ARAR), and provide a qualitative assessment of the risks associated with the sites. This assessment includes consideration of whether contaminant concentrations pose a risk to human health or the environment that warrants action through IRM. Seven sites were intrusively investigated: 1322-N, 116-N-2, 119-N, 120-N-2, 120-N-1, South Settling Pond, and the 166-N Tank Farm (UN-100-N-17) unplanned release at the 166-N Tank Farm. Vadose zone sediments from these sites were sampled and analyzed. Radiological contamination is the primary concern as confirmed through this study

  17. Origins of domestication and polyploidy in oca (Oxalis tuberosa : Oxalidaceae): nrDNA ITS data.

    Science.gov (United States)

    Emshwiller, E; Doyle, J

    1998-07-01

    As part of a study aimed at elucidating the origins of the octoploid tuber crop "oca," Oxalis tuberosa, DNA sequences of the internal trancribed spacer of nuclear ribosomal DNA (nrDNA ITS) were determined for oca and several wild Oxalis species, mostly from Bolivia. Phylogenetic analysis of these data supports a group of these species as being close relatives of oca, in agreement with morphology and cytology, but at odds with traditional infrageneric taxonomy. Variation in ITS sequences within this group is quite low (0-7 substitutions in the entire ITS region), contrasting with the highly divergent (unalignable in some cases) sequences within the genus overall. Some groups of morphologically differentiated species were found to have identical sequences, notably a group that includes oca, wild populations of Oxalis that bear small tubers, and several other clearly distinct species. The presence of a second, minor sequence type in at least some oca accessions suggests a possible contribution from a second genome donor, also from within this same species group. ITS data lack sufficient variation to elucidate the origins of oca precisely, but have identified a pool of candidate species and so can be used as a tool to screen yet unsampled species for possible progenitors.

  18. Seasonality in polyps of a tropical cubozoan: A latina nr mordens.

    Directory of Open Access Journals (Sweden)

    Robert Courtney

    Full Text Available A latina nr mordens have been located in large predictable spawning aggregations near Osprey Reef in the Coral Sea eight to ten days after a full moon; however, polyps have never been located in-situ. The polyp stage contributes to the abundance of medusae through asexual reproduction and metamorphosis, and may influence the periodicity of medusae by metamorphosis of the polyp. To elucidate the relationship between medusae periodicity and polyp ecology, polyps were exposed to thermal and osmotic treatments in order to determine the theoretical environmental limits to their distribution. Maximum fecundity occurred in thermal treatments of 21 to 25ºC and the theoretical minimum thermal requirement for population stability was approximately 17ºC. Polyps were also exposed to five feeding regimes and fecundity was found to be positively correlated with feeding frequency. Thermal and osmotic variations did not induce metamorphosis in this species, however, reduced food did. The implications of asexual reproduction and cues for metamorphosis in relation to population dynamics of this species are discussed.

  19. [Carl Schirren als Gelehrter im Spannungsfeld von Wissenschaft und politischer Publizistik. 13 Beiträge zum 22. Baltischen Seminar 2010 ; Carl Schirren. Vorlesungen über livländische Geschichte. Nachschrift von Johannes Lossius] / Karsten Brügge

    Index Scriptorium Estoniae

    Brüggemann, Karsten, 1965-

    2015-01-01

    Arvustus: Carl Schirren als Gelehrter im Spannungsfeld von Wissenschaft und politischer Publizistik. 13 Beiträge zum 22. Baltischen Seminar 2010 (Baltische Seminare, 20). Hrsg. von Michael Garleff. Verlag Carl-Schirren-Gesellschaft. Lüneburg 2013; Carl Schirren. Vorlesungen über livländische Geschichte. Nachschrift von Johannes Lossius (Baltische Seminare, 20-1). Hrsg. von Carl Schirren und Wilhelm Lenz. Verlag Carl-Schirren-Gesellschaft. Lüneburg 2013

  20. Third-party access zu district-heating grids. Considerations to the range of paragraph 19 sect. 4 no. 4 GWB and to the relation of antitrust law and energy law; Drittzugang zu Fernwaermenetzen. Ueberlegungen zur Reichweite des paragraph 19 Abs. 4 Nr. 4 GWB und zum Verhaeltnis von Kartell- und Energierecht

    Energy Technology Data Exchange (ETDEWEB)

    Koerber, Torsten

    2011-07-01

    The establishment of a sector-specific network access regulation significantly contributes to the development of vigorous competition in telecommunications services. Even in the sectors of electricity and natural gas hopes are placed on a regulation. Recognising this, in early 2010, a contract for a legal opinion concluded on behalf of the Energieeffizienzverband fuer Waerme, Kaelte und KWK e.V. (Frankfurt/Main, Federal Republic of Germany). This legal opinion should examine the following questions: (1) To what extent can the effect of the substitution ban also be used for the enforcement of access requirements?; (2) Is the heat supply system a key organization in line with paragraph 19 Sect. 4 No 4 GWB (GWB = Restriction of Competition)? (3) May the network access be denied on economic grounds in the way of objective justification? (4) How far may in particular environmental and beyond considerations of economic standing aspects be used? Based on these particular issues, especially, grid access requirements are traced. The author of the contribution under consideration investigates the following issues: (a) To what extent can claims on supply with or acceptance of district heating be derived from anti-trust standards?; (b) What are the impacts of the specialties of district heating on the application of the antitrust law? (d) To what extent does the antitrust law interact with the regulations of energy law and its objectives?.

  1. BDE-47 causes developmental retardation with down-regulated expression profiles of ecdysteroid signaling pathway-involved nuclear receptor (NR) genes in the copepod Tigriopus japonicus

    Energy Technology Data Exchange (ETDEWEB)

    Hwang, Dae-Sik; Han, Jeonghoon; Won, Eun-Ji; Kim, Duck-Hyun; Jeong, Chang-Bum [Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419 (Korea, Republic of); Hwang, Un-Ki [Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Fisheries Research & Development Institute, Incheon 46083 (Korea, Republic of); Zhou, Bingsheng [State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072 (China); Choe, Joonho [Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141 (Korea, Republic of); Lee, Jae-Seong, E-mail: jslee2@skku.edu [Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419 (Korea, Republic of)

    2016-08-15

    Highlights: • The developmental rate was significantly inhibited (P < 0.05) in response to BDE-47. • Expression profiles of nearly all NR genes were the highest at naupliar stages 5–6. • USP, HR96, and FTZ-F1 genes showed significant sex differences (P < 0.05) over different developmental stages. • NR gene expression patterns showed significant decreases (P<0.05) in response to BDE-47. • BDE-47 leads to molting and metamorphosis retardation and suppresses transcription of NR genes. - Abstract: 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47) is a persistent organic pollutant (POP) in marine environments. Despite its adverse effects (e.g. developmental retardation) in ecdysozoa, the effects of BDE-47 on transcription of ecdysteroid signaling pathway-involved-nuclear receptor (NR) genes and metamorphosis-related genes have not been examined in copepods. To examine the deleterious effect of BDE-47 on copepod molting and metamorphosis, BDE-47 was exposed to the harpacticoid copepod Tigriopus japonicus, followed by monitoring developmental retardation and transcriptional alteration of NR genes. The developmental rate was significantly inhibited (P < 0.05) in response to BDE-47 and the agricultural insecticide gamma-hexachlorocyclohexane. Conversely, the ecdysteroid agonist ponasterone A (PoA) led to decreased molting and metamorphosis time (P < 0.05) from the nauplius stage to the adult stage. In particular, expression profiles of all NR genes were the highest at naupliar stages 5–6 except for SVP, FTZ-F1, and HR96 genes. Nuclear receptor USP, HR96, and FTZ-F1 genes also showed significant sex differences (P < 0.05) in gene expression levels over different developmental stages, indicating that these genes may be involved in vitellogenesis. NR gene expression patterns showed significant decreases (P < 0.05) in response to BDE-47 exposure, implying that molting and metamorphosis retardation is likely associated with NR gene expression. In summary, BDE-47

  2. BDE-47 causes developmental retardation with down-regulated expression profiles of ecdysteroid signaling pathway-involved nuclear receptor (NR) genes in the copepod Tigriopus japonicus

    International Nuclear Information System (INIS)

    Hwang, Dae-Sik; Han, Jeonghoon; Won, Eun-Ji; Kim, Duck-Hyun; Jeong, Chang-Bum; Hwang, Un-Ki; Zhou, Bingsheng; Choe, Joonho; Lee, Jae-Seong

    2016-01-01

    Highlights: • The developmental rate was significantly inhibited (P < 0.05) in response to BDE-47. • Expression profiles of nearly all NR genes were the highest at naupliar stages 5–6. • USP, HR96, and FTZ-F1 genes showed significant sex differences (P < 0.05) over different developmental stages. • NR gene expression patterns showed significant decreases (P<0.05) in response to BDE-47. • BDE-47 leads to molting and metamorphosis retardation and suppresses transcription of NR genes. - Abstract: 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47) is a persistent organic pollutant (POP) in marine environments. Despite its adverse effects (e.g. developmental retardation) in ecdysozoa, the effects of BDE-47 on transcription of ecdysteroid signaling pathway-involved-nuclear receptor (NR) genes and metamorphosis-related genes have not been examined in copepods. To examine the deleterious effect of BDE-47 on copepod molting and metamorphosis, BDE-47 was exposed to the harpacticoid copepod Tigriopus japonicus, followed by monitoring developmental retardation and transcriptional alteration of NR genes. The developmental rate was significantly inhibited (P < 0.05) in response to BDE-47 and the agricultural insecticide gamma-hexachlorocyclohexane. Conversely, the ecdysteroid agonist ponasterone A (PoA) led to decreased molting and metamorphosis time (P < 0.05) from the nauplius stage to the adult stage. In particular, expression profiles of all NR genes were the highest at naupliar stages 5–6 except for SVP, FTZ-F1, and HR96 genes. Nuclear receptor USP, HR96, and FTZ-F1 genes also showed significant sex differences (P < 0.05) in gene expression levels over different developmental stages, indicating that these genes may be involved in vitellogenesis. NR gene expression patterns showed significant decreases (P < 0.05) in response to BDE-47 exposure, implying that molting and metamorphosis retardation is likely associated with NR gene expression. In summary, BDE-47

  3. Estradiol-induced increase in the magnitude of long-term potentiation is prevented by blocking NR2B-containing receptors.

    Science.gov (United States)

    Smith, Caroline C; McMahon, Lori L

    2006-08-16

    Estradiol, through activation of genomic estrogen receptors, induces changes in synaptic morphology and function in hippocampus, a brain region important for memory acquisition. Specifically, this hormone increases CA1 pyramidal cell dendritic spine density, NMDA receptor (NMDAR)-mediated transmission, and the magnitude of long-term potentiation (LTP) at CA3-CA1 synapses. We recently reported that the estradiol-induced increase in LTP magnitude occurs only when there is a simultaneous increase in the fractional contribution of NMDAR-mediated transmission relative to AMPA receptor transmission, suggesting a direct role for the increase in NMDAR transmission to the heightened LTP magnitude. Estradiol has been shown to increase expression of the NMDAR subunit NR2B, but whether this translates into an increase in function of NR2B-containing receptors remains to be determined. Here we show that not only is the estradiol-induced increase in NMDAR transmission mediated by NR2B-containing receptors, but blocking these receptors using RO25-6981 [R-(R,S)-alpha-(4-hydroxyphenyl)-beta-methyl-4-(phenylmethyl)-1-piperidine propranol] (0.5 microM), an NR2B selective antagonist, prevents the estradiol-induced increase in LTP magnitude. Thus, our data show a causal link between the estradiol-induced increase in transmission mediated by NR2B-containing NMDARs and the increase in LTP magnitude.

  4. NR4A orphan nuclear receptors influence retinoic acid and docosahexaenoic acid signaling via up-regulation of fatty acid binding protein 5

    International Nuclear Information System (INIS)

    Volakakis, Nikolaos; Joodmardi, Eliza; Perlmann, Thomas

    2009-01-01

    The orphan nuclear receptor (NR) Nurr1 is expressed in the developing and adult nervous system and is also induced as an immediate early gene in a variety of cell types. In silico analysis of human promoters identified fatty acid binding protein 5 (FABP5), a protein shown to enhance retinoic acid-mediated PPARβ/δ signaling, as a potential Nurr1 target gene. Nurr1 has previously been implicated in retinoid signaling via its heterodimerization partner RXR. Since NRs are commonly involved in cross-regulatory control we decided to further investigate the regulatory relationship between Nurr1 and FABP5. FABP5 expression was up-regulated by Nurr1 and other NR4A NRs in HEK293 cells, and Nurr1 was shown to activate and bind to the FABP5 promoter, supporting that FABP5 is a direct downstream target of NR4A NRs. We also show that the RXR ligand docosahexaenoic acid (DHA) can induce nuclear translocation of FABP5. Moreover, via up-regulation of FABP5 Nurr1 can enhance retinoic acid-induced signaling of PPARβ/δ and DHA-induced activation of RXR. We also found that other members of the NR4A orphan NRs can up-regulate FABP5. Thus, our findings suggest that NR4A orphan NRs can influence signaling events of other NRs via control of FABP5 expression levels.

  5. NR4A orphan nuclear receptors influence retinoic acid and docosahexaenoic acid signaling via up-regulation of fatty acid binding protein 5

    Energy Technology Data Exchange (ETDEWEB)

    Volakakis, Nikolaos; Joodmardi, Eliza [Ludwig Institute for Cancer Research Ltd., Box 240, S-17177 Stockholm (Sweden); Perlmann, Thomas, E-mail: thomas.perlmann@licr.ki.se [Ludwig Institute for Cancer Research Ltd., Box 240, S-17177 Stockholm (Sweden); The Department of Cell and Molecular Biology, Karolinska Institute, S-17177 Stockholm (Sweden)

    2009-12-25

    The orphan nuclear receptor (NR) Nurr1 is expressed in the developing and adult nervous system and is also induced as an immediate early gene in a variety of cell types. In silico analysis of human promoters identified fatty acid binding protein 5 (FABP5), a protein shown to enhance retinoic acid-mediated PPAR{beta}/{delta} signaling, as a potential Nurr1 target gene. Nurr1 has previously been implicated in retinoid signaling via its heterodimerization partner RXR. Since NRs are commonly involved in cross-regulatory control we decided to further investigate the regulatory relationship between Nurr1 and FABP5. FABP5 expression was up-regulated by Nurr1 and other NR4A NRs in HEK293 cells, and Nurr1 was shown to activate and bind to the FABP5 promoter, supporting that FABP5 is a direct downstream target of NR4A NRs. We also show that the RXR ligand docosahexaenoic acid (DHA) can induce nuclear translocation of FABP5. Moreover, via up-regulation of FABP5 Nurr1 can enhance retinoic acid-induced signaling of PPAR{beta}/{delta} and DHA-induced activation of RXR. We also found that other members of the NR4A orphan NRs can up-regulate FABP5. Thus, our findings suggest that NR4A orphan NRs can influence signaling events of other NRs via control of FABP5 expression levels.

  6. [Applylication of new type combined fragments: nrDNA ITS+ nad 1-intron 2 for identification of Dendrobium species of Fengdous].

    Science.gov (United States)

    Geng, Li-xia; Zheng, Rui; Ren, Jie; Niu, Zhi-tao; Sun, Yu-long; Xue, Qing-yun; Liu, Wei; Ding, Xiao-yu

    2015-08-01

    In this study, 17 kinds of Dendrobium species of Fengdous including 39 individuals were collected from 4 provinces. Mitochondrial gene sequences co I, nad 5, nad 1-intron 2 and chloroplast gene sequences rbcL, matK amd psbA-trnH were amplified from these materials, as well as nrDNA ITS. Furthermore, suitable sequences for identification of Dendrobium species of Fengdous were screened by K-2-P and P-distance. The results showed that during the mentioned 7 sequences, nrDNA ITS, nad 1-intron 2 and psbA-trnH which had a high degree of variability could be used to identify Dendrobium species of Fengdous. However, single fragment could not be used to distinguish D. moniliforme and D. huoshanense. Moreover, compared to other combined fragments, new type combined fragments nrDNA ITS+nad 1-intron 2 was more effective in identifying the original plants of Dendrobium species and could be used to identify D. huoshanense and D. moniliforme. Besides, according to the UPGMA tree constructed with nrDNA ITS+nad 1-intron 2, 3 inspected Dendrobium plants were identified as D. huoshanense, D. moniliforme and D. officinale, respectively. This study identified Dendrobium species of Fengdous by combined fragments nrDNA ITS+nad 1-intron 2 for the first time, which provided a more effective basis for identification of Dendrobium species. And this study will be helpful for regulating the market of Fengdous.

  7. Monosialotetrahexosylganglioside Inhibits the Expression of p-CREB and NR2B in the Auditory Cortex in Rats with Salicylate-Induced Tinnitus.

    Science.gov (United States)

    Song, Rui-Biao; Lou, Wei-Hua

    2015-01-01

    This study investigated the effects of monosialotetrahexosylganglioside (GM1) on the expression of N-methyl-D-aspartate receptor subunit 2B (NR2B) and phosphorylated (p)-cyclic AMP response element-binding protein (CREB) in the auditory cortex of rats with tinnitus. Tinnitus-like behavior in rats was tested with the gap prepulse inhibition of acoustic startle paradigm. We then investigated the NR2B mRNA and protein and p-CREB protein levels in the auditory cortex of tinnitus rats compared with normal rats. Rats treated for 4 days with salicylate exhibited tinnitus. NR2B mRNA and protein and p-CREB protein levels were upregulated in these animals, with expression returning to normal levels 14 days after cessation of treatment; baseline levels of NR2B and p-CREB were also restored by GM1 administration. These data suggest that chronic salicylate administration induces tinnitus via upregulation of p-CREB and NR2B expression, and that GM1 can potentially be used to treat tinnitus.

  8. Efeito de um resíduo do processo de galvanoplastia sobre a vulcanização da borracha natural (NR Effect from the waste from the electroplating process on the natural rubber (NR vulcanization

    Directory of Open Access Journals (Sweden)

    Alex C. S. Santos

    2009-01-01

    Full Text Available Um resíduo gerado no processo de galvanoplastia (RG, rico em metais, foi incorporado em composições de borracha natural (NR em substituição parcial ou total ao ativador comum do processo de vulcanização, o óxido de zinco (ZnO. As diferentes misturas foram preparadas em um misturador de calandras e, após a reometria, foram vulcanizadas a 150 °C. As propriedades mecânicas de resistência à tração e rasgo foram determinadas, além do teor de gel. Além disso, o efeito do resíduo RG sobre a cinética de vulcanização foi analisado através do modelo de Coran, usando-se as temperaturas de 150, 160 e 170 °C. Os resultados mostraram que RG desenvolve algum efeito catalítico sobre a vulcanização de NR, embora exista uma considerável perda na resistência à tração quando o teor de RG excede o do ZnO nas composições. Quanto ao rasgo, a propriedade é mantida em um patamar satisfatório enquanto teores menores de RG são utilizados.A waste generated in the electroplating process (RG, rich in metals, was incorporated in natural rubber (NR compositions, as a partial or complete replacement of zinc oxide (ZnO, which is a typical activator of the vulcanization process. Different mixtures were prepared in a two-roll mixer, which were vulcanized at 150 ºC after the rheometry measurements. Mechanical properties such as tensile and tear strength were evaluated, as well as the gel content. In addition, the effect of RG waste upon the vulcanization kinetics was analyzed with the Coran model using temperatures at 150, 160 and 170 ºC. The results showed that RG develops some catalytic effect upon NR vulcanization, but there is a poor mechanical performance when RG loading is higher than that of ZnO in the compositions. For the tear strength, the property is maintained at a reasonable level if a small RG loading is utilized.

  9. Auf dem Weg zum Web 3.0: Taxonomien und Ontologien für die medizinische Ausbildung - eine systematische Literaturrecherche [Towards Web 3.0: Taxonomies and ontologies for medical education - a systematic review

    Directory of Open Access Journals (Sweden)

    Blaum, Wolf E.

    2013-02-01

    Curriculumsentwicklung und –kartierung als auch zur Orientierung innerhalb der seit Jahren zunehmenden Fülle von Lernressourcen in der medizinischen Ausbildung stellt das semantische Web („Web 3.0“ ein niedrigschwelliges, effektives Hilfsmittel dar, das es ermöglicht, inhaltlich verwandte Elemente über Systemgrenzen hinweg zu identifizieren. Voraussetzung dafür ist der Einsatz eines geeigneten strukturierten Vokabulars zur maschinenlesbaren, inhaltlichen Beschreibung von Objekten, um die bisher notwendige manuelle Verknüpfung durch eine automatisch erzeugte, inhaltsbasierte bzw. semantische Verknüpfung zu ersetzen. Ziel dieser Arbeit ist es, existierende Taxonomien und Ontologien zur Annotation medizinischer Lernressourcen und -inhalte zusammenzustellen und anhand ausgewählter Kriterien zu vergleichen sowie auf ihre Eignung zum Einsatz im eingangs beschriebenen Kontext zu überprüfen.Methoden: Anhand einer systematischen Literaturrecherche wurden existierende Taxonomien und Ontologien zur Beschreibung medizinischer Lernressourcen identifiziert. Für jedes so identifizierte strukturierte Vokabular wurden mittels Websuche und/oder Kontakt zu den Herausgebern Thema, Struktur, Sprache, Umfang, Wartung und Technik der Taxonomie/Ontologie ermittelt und deren Eignung für den Einsatz im semantischen Web überprüft.Ergebnisse: In 20 identifizierten Publikationen wurden 14 strukturierte Vokabulare identifiziert, die sich in Sprache, Umfang, Aktualität und Wartung zum Teil sehr stark unterschieden. Keines der identifizierten Vokabulare erfüllte die erforderlichen Kriterien zur inhaltlichen Beschreibung medizinischer Ausbildungsinhalte und Lernressourcen im deutschsprachigen Raum.Diskussion: Auf dem Weg zum Web 3.0 stellen Auswahl und Einsatz eines geeigneten, deutschsprachigen Vokabulars zur maschinenlesbaren, inhaltlichen Beschreibung von Objekten ein relevantes Problem dar. Mögliche Lösungsansätze umfassen die Neuentwicklung, die Übersetzung und/ oder die Kombination

  10. Eduard Tubina "Kogutud teoste" köidetest I/I (sümfooniad nr. 1 ja 2) ning I/VII (orkestrisüidid) / Timo Virtanen ; (tõlkinud Merike Vaitmaa)

    Index Scriptorium Estoniae

    Virtanen, Timo, 1956-

    2013-01-01

    Arvustus: Lauri Sirp, Toomas Trass (toim.). Kogutud teosed. I seeria, I köide : Sümfoonia nr. 1 ; Sümfoonia nr. 2 : Legendaarne / Eduard Tubin. Stockholm : Rahvusvaheline Eduard Tubina Ühing ; Gehrmans Musikförlag, 2012

  11. New truncation mutation of the NR2E3 gene in a Japanese patient with enhanced S-cone syndrome.

    Science.gov (United States)

    Kuniyoshi, Kazuki; Hayashi, Takaaki; Sakuramoto, Hiroyuki; Mishima, Hiroshi; Tsuneoka, Hiroshi; Tsunoda, Kazushige; Iwata, Takeshi; Shimomura, Yoshikazu

    2016-11-01

    The enhanced S-cone syndrome (ESCS) is a rare hereditary retinal degeneration that has enhanced short wavelength-sensitive cone (S-cone) functions. The longitudinal clinical course of this disease has been rarely reported, and the genetic aspects of ESCS have not been well investigated in the Japanese population. In this report, we present our clinical and genetic findings for 2 patients with ESCS. The patients were 2 unrelated Japanese men. Standard ophthalmic examinations and mutation screening for the NR2E3 gene were performed. Patient 1 was a 36-year-old man, and his clinical findings were typical of ESCS. His decimal best-corrected visual acuity (BCVA) was 1.0 OD and 0.5 OS after removal of cataracts. Genetic investigations revealed a homozygous truncation frameshift, the p.I307LfsX33 mutation. Patient 2 was an 11-year-old boy when he was first examined by us. His clinical findings were typical of ESCS except for uveitis in the left eye. His decimal BCVA at the age of 39 years was maintained at 1.5 in each eye, although the retinal degeneration and visual field impairments had progressed during the follow-up period. The genetic investigations revealed homozygous mutations of p.R104Q in the NR2E3 gene. The frameshift mutation, p.I307LfsX33, in the NR2E3 gene is a new causative mutation for ESCS. The clinical observations for patient 2 are the longest ever reported. The retinal degeneration caused by this mutation is slowly progressive, and these patients maintained good vision with maintenance of the foveal structure until their late thirties.

  12. NR4A nuclear receptors mediate carnitine palmitoyltransferase 1A gene expression by the rexinoid HX600

    Energy Technology Data Exchange (ETDEWEB)

    Ishizawa, Michiyasu [Division of Biochemistry, Department of Biomedical Sciences, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610 (Japan); Kagechika, Hiroyuki [Graduate School of Biomedical Science, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062 (Japan); Makishima, Makoto, E-mail: makishima.makoto@nihon-u.ac.jp [Division of Biochemistry, Department of Biomedical Sciences, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610 (Japan)

    2012-02-24

    Highlights: Black-Right-Pointing-Pointer The function of RXR heterodimers with NR4 receptors remains unknown. Black-Right-Pointing-Pointer The RXR ligand HX600 induces expression of carnitine palmitoyltransferase 1A (CPT1A). Black-Right-Pointing-Pointer HX600-induced CPT1A expression is mediated by the NR4 receptors, Nur77 and NURR1. Black-Right-Pointing-Pointer CPT1A induction by HX600 is not mediated by de novo protein synthesis. Black-Right-Pointing-Pointer CPT1A could be a target of the Nur77-RXR and NURR1-RXR heterodimers. -- Abstract: Retinoid X receptors (RXRs) are members of the nuclear receptor superfamily and can be activated by 9-cis retinoic acid (9CRA). RXRs form homodimers and heterodimers with other nuclear receptors such as the retinoic acid receptor and NR4 subfamily nuclear receptors, Nur77 and NURR1. Potential physiological roles of the Nur77-RXR and NURR1-RXR heterodimers have not been elucidated. In this study, we identified a gene regulated by these heterodimers utilizing HX600, a selective RXR agonist for Nur77-RXR and NURR1-RXR. While 9CRA induced many genes, including RAR-target genes, HX600 effectively induced only carnitine palmitoyltransferase 1A (CPT1A) in human teratocarcinoma NT2/D1 cells, which express RXR{alpha}, Nur77 and NURR1. HX600 also increased CPT1A expression in human embryonic kidney (HEK) 293 cells and hepatocyte-derived HepG2 cells. Although HX600 induced CPT1A less effectively than 9CRA, overexpression of Nur77 or NURR1 increased the HX600 response to levels similar to 9CRA in NT2/D1 and HEK293 cells. A dominant-negative form of Nur77 or NURR1 repressed the induction of CPT1A by HX600. A protein synthesis inhibitor did not alter HX600-dependent CPT1A induction. Thus, the rexinoid HX600 directly induces expression of CPT1A through a Nur77 or NURR1-mediated mechanism. CPT1A, a gene involved in fatty acid {beta}-oxidation, could be a target of RXR-NR4 receptor heterodimers.

  13. Fatigue Resistance of Filled NR with PMMA-Wrapped and Rare Earth-Doped Alumina-Siloxane Gel

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Poly (methyl methacrylate) (PMMA) was used to wrap alumina-siloxane sol which was produced by water glass, aluminum nitrate and α-methacrylic acid, and as a result, alumina-siloxane gel wrapped by PMMA was obtained. Meanwhile, rare earth ions were employed to dope in the course of reaction, and the formed rare earth doped PMMA microcapsule powder was filled into natural rubber (NR). It is found through the analysis of mechanical properties that Young′s modulus universally improves and a remarkable resistance to fatigue is displayed. Retention rate of tensile strength is twice that of the controlled sample after ten thousand times of extension fatigue.

  14. Liquid natural rubber (LNR) as a compatibiliser in NR/LLDPE blends: the mixing and mechanical property studies

    International Nuclear Information System (INIS)

    Dahlan Mohd; Khairul Zaman Mohd Dahlan; Ibrahim Abdullah

    2000-01-01

    The plastograms of mixing were able to give information on the compatibility of NR/LLDPE 60/40 blends studied. Using the conversion method, torques obtained from a plasticorder mixer could be converted to standard rheological properties in order to investigate fusing behaviour of the blends as the results of LNR additions. Improvements in the mechanical properties such as tensile, elongation and hardness were found in consonance with the increase in gel contents of the blends. The additions of LNR in the blends were able to reduce the interfacial tension, therefore improved the interaction between the phases of the blends. (author)

  15. A novel experimental approach for the determination of the photooxidative decay of semivolatile pesticides and POPs adsorbed on single levitated particles; Aufbau eines Messverfahrens zum photo-oxidativen Abbau von semivolatilen Pflanzenschutzmitteln und POPs an levitierten Einzelpartikeln

    Energy Technology Data Exchange (ETDEWEB)

    Ruehl, E.

    2002-08-01

    gespeicherte Mikro- und Nanopartikel, auf deren Oberflaeche die zu untersuchenden Substanzen in Monolagen adsorbiert sind. Die beruehrungslose Speicherung der Partikel gelingt in einer elektrodynamischen Falle, in der die Temperatur, Luftdruck, Luftfeuchte und die Zusammensetzung der Atmosphaere kontrolliert einstellbar ist. Die Partikel werden reaktiven Gasen, wie OH-Radikalen, ausgesetzt. Die induzierten Veraenderungen der adsorbierten Substanzen werden zeitaufgeloest mit Hilfe der Raman-Spektroskopie verfolgt, so dass chemische Prozesse, die entstehenden Reaktionsprodukte sowie auch Abdampfverluste von der Partikeloberflaeche quantifiziert werden koennen. Alternativ kann auch die Fourier-Transform-Infrarot-Spektroskopie zum spezifischen Nachweis genutzt werden. Mit den Experimenten lassen sich reaktionskinetische Daten zum indirekten Photoabbau schwerfluechtiger Substanzen als Funktion der Umgebungsbedingungen ableiten. Im Zuge des Vorhabens wurden die notwendigen experimentellen Voraussetzungen geschaffen und die hierfuer konstruierten Komponenten anhand von Testsystemen im Hinblick auf ihre Einsetzbarkeit charakterisiert. Erste Experimente zur Reaktionskinetik belegen die Funktionstuechtigkeit des neuen Verfahrens. (orig.)

  16. Chronic Administration of Benzo(apyrene Induces Memory Impairment and Anxiety-Like Behavior and Increases of NR2B DNA Methylation.

    Directory of Open Access Journals (Sweden)

    Wenping Zhang

    Full Text Available Recently, an increasing number of human and animal studies have reported that exposure to benzo(apyrene (BaP induces neurological abnormalities and is also associated with adverse effects, such as tumor formation, immunosuppression, teratogenicity, and hormonal disorders. However, the exact mechanisms underlying BaP-induced impairment of neurological function remain unclear. The aim of this study was to examine the regulating mechanisms underlying the impact of chronic BaP exposure on neurobehavioral performance.C57BL mice received either BaP in different doses (1.0, 2.5, 6.25 mg/kg or olive oil twice a week for 90 days. Memory and emotional behaviors were evaluated using Y-maze and open-field tests, respectively. Furthermore, levels of mRNA expression were measured by using qPCR, and DNA methylation of NMDA receptor 2B subunit (NR2B was examined using bisulfate pyrosequencing in the prefrontal cortex and hippocampus.Compared to controls, mice that received BaP (2.5, 6.25 mg/kg showed deficits in short-term memory and an anxiety-like behavior. These behavioral alterations were associated with a down-regulation of the NR2B gene and a concomitant increase in the level of DNA methylation in the NR2B promoter in the two brain regions.Chronic BaP exposure induces an increase in DNA methylation in the NR2B gene promoter and down-regulates NR2B expression, which may contribute to its neurotoxic effects on behavioral performance. The results suggest that NR2B vulnerability represents a target for environmental toxicants in the brain.

  17. BDE-47 causes developmental retardation with down-regulated expression profiles of ecdysteroid signaling pathway-involved nuclear receptor (NR) genes in the copepod Tigriopus japonicus.

    Science.gov (United States)

    Hwang, Dae-Sik; Han, Jeonghoon; Won, Eun-Ji; Kim, Duck-Hyun; Jeong, Chang-Bum; Hwang, Un-Ki; Zhou, Bingsheng; Choe, Joonho; Lee, Jae-Seong

    2016-08-01

    2,2',4,4'-Tetrabromodiphenyl ether (BDE-47) is a persistent organic pollutant (POP) in marine environments. Despite its adverse effects (e.g. developmental retardation) in ecdysozoa, the effects of BDE-47 on transcription of ecdysteroid signaling pathway-involved-nuclear receptor (NR) genes and metamorphosis-related genes have not been examined in copepods. To examine the deleterious effect of BDE-47 on copepod molting and metamorphosis, BDE-47 was exposed to the harpacticoid copepod Tigriopus japonicus, followed by monitoring developmental retardation and transcriptional alteration of NR genes. The developmental rate was significantly inhibited (P<0.05) in response to BDE-47 and the agricultural insecticide gamma-hexachlorocyclohexane. Conversely, the ecdysteroid agonist ponasterone A (PoA) led to decreased molting and metamorphosis time (P<0.05) from the nauplius stage to the adult stage. In particular, expression profiles of all NR genes were the highest at naupliar stages 5-6 except for SVP, FTZ-F1, and HR96 genes. Nuclear receptor USP, HR96, and FTZ-F1 genes also showed significant sex differences (P<0.05) in gene expression levels over different developmental stages, indicating that these genes may be involved in vitellogenesis. NR gene expression patterns showed significant decreases (P<0.05) in response to BDE-47 exposure, implying that molting and metamorphosis retardation is likely associated with NR gene expression. In summary, BDE-47 leads to molting and metamorphosis retardation and suppresses transcription of NR genes. This information will be helpful in understanding the molting and metamorphosis delay mechanism in response to BDE-47 exposure. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Erikontingendi filtreerimine ja töölerakendamine Eesti NSV-s kontroll-filterlaager nr. 0316 näitel / Peeter Kaasik

    Index Scriptorium Estoniae

    Kaasik, Peeter, 1974-

    2010-01-01

    1945. aasta alguses paigutati Paldiski lähedale Põllkülasse NKVD erilaager. Laager allus NSV Liidu NKVD kontroll-filterlaagrite osakonnale. Erikontingendi mõistest. Kontroll-filterlaagri nr. 0316 rajamisest ja struktuurist. Laagri Smerši osakonna tegevusest. Kontroll-laager nr. 0316 oli mõneti erandlik, sealt ei saadetud isikuid üldjuhul Eestist välja. Balti riikide kodanikest erikontingendi küsimus jäi täpselt määratlemata, NSV Liidu kui oma kodumaa reeturiteks ei saanud neid nimetada

  19. Qualified and Unqualified (N-R C) mental health nursing staff--minor differences in sources of stress and burnout. A European multi-centre study.

    Science.gov (United States)

    Sorgaard, Knut W; Ryan, Peter; Dawson, Ian

    2010-06-14

    Unqualified/non-registered caregivers (N-R Cs) will continue to play important roles in the mental health services. This study compares levels of burnout and sources of stress among qualified and N-R Cs working in acute mental health care. A total of 196 nursing staff --124 qualified staff (mainly nurses) and 72 N-R Cs with a variety of different educational backgrounds--working in acute wards or community mental teams from 5 European countries filled out the Maslach Burnout Inventory (MBI), the Mental Health Professional Scale (MHPSS) and the Psychosocial Work Environment and Stress Questionnaire (PWSQ). (a) The univariate differences were generally small and restricted to a few variables. Only Social relations (N-R Cs being less satisfied) at Work demands (nurses reporting higher demands) were different at the .05 level. (b) The absolute scores both groups was highest on variables that measured feelings of not being able to influence a work situation characterised by great demands and insufficient resources. Routines and educational programs for dealing with stress should be available on a routine basis. (c) Multivariate analyses identified three extreme groups: (i) a small group dominated by unqualified staff with high depersonalization, (ii) a large group that was low on depersonalisation and high on work demands with a majority of qualified staff, and (iii) a small N-R C-dominated group (low depersonalization, low work demands) with high scores on professional self-doubt. In contrast to (ii) the small and N-R C-dominated groups in (i) and (iii) reflected mainly centre-dependent problems. The differences in burnout and sources of stress between the two groups were generally small. With the exception of high work demands the main differences between the two groups appeared to be centre-dependent. High work demands characterized primarily qualified staff. The main implication of the study is that no special measures addressed towards N-R Cs in general with regard

  20. NR4A1 (Nur77 mediates thyrotropin-releasing hormone-induced stimulation of transcription of the thyrotropin β gene: analysis of TRH knockout mice.

    Directory of Open Access Journals (Sweden)

    Yasuyo Nakajima

    Full Text Available Thyrotropin-releasing hormone (TRH is a major stimulator of thyrotropin-stimulating hormone (TSH synthesis in the anterior pituitary, though precisely how TRH stimulates the TSHβ gene remains unclear. Analysis of TRH-deficient mice differing in thyroid hormone status demonstrated that TRH was critical for the basal activity and responsiveness to thyroid hormone of the TSHβ gene. cDNA microarray and K-means cluster analyses with pituitaries from wild-type mice, TRH-deficient mice and TRH-deficient mice with thyroid hormone replacement revealed that the largest and most consistent decrease in expression in the absence of TRH and on supplementation with thyroid hormone was shown by the TSHβ gene, and the NR4A1 gene belonged to the same cluster as and showed a similar expression profile to the TSHβ gene. Immunohistochemical analysis demonstrated that NR4A1 was expressed not only in ACTH- and FSH- producing cells but also in thyrotrophs and the expression was remarkably reduced in TRH-deficient pituitary. Furthermore, experiments in vitro demonstrated that incubation with TRH in GH4C1 cells increased the endogenous NR4A1 mRNA level by approximately 50-fold within one hour, and this stimulation was inhibited by inhibitors for PKC and ERK1/2. Western blot analysis confirmed that TRH increased NR4A1 expression within 2 h. A series of deletions of the promoter demonstrated that the region between bp -138 and +37 of the TSHβ gene was responsible for the TRH-induced stimulation, and Chip analysis revealed that NR4A1 was recruited to this region. Conversely, knockdown of NR4A1 by siRNA led to a significant reduction in TRH-induced TSHβ promoter activity. Furthermore, TRH stimulated NR4A1 promoter activity through the TRH receptor. These findings demonstrated that 1 TRH is a highly specific regulator of the TSHβ gene, and 2 TRH mediated induction of the TSHβ gene, at least in part by sequential stimulation of the NR4A1-TSHβ genes through a PKC and

  1. The roles of liquid epoxidized natural rubber acrylate (LENRA) as compatibiliser in NR/HDPE blends: structural studies by electron microscopy and SANS techniques

    International Nuclear Information System (INIS)

    Dahlan Mohd; Mahathir Mohamed; Abdul Aziz Mohamed

    2006-01-01

    Further works have been carried out to determine the roles of LENRA in inducing compatibility between immiscible blends of NR/HDPE 60/40. In addition to electron microscopy images, SANS technique has been used to determine the interface thickness between the blend components. Phase-separated structures of HDPE domain embedded in NR matrix were observed by electron microscopy technique. From the results so far, the combination of 15% LENRA and 200 kGy EB radiation gives us the best Porod plot indicating the more meaningful SANS results. (Author)

  2. Effect of heterogeneous distribution of crosslink density on physical properties of radiation vulcanized NR (Natural Rubber) latex film

    International Nuclear Information System (INIS)

    Keizo Makuuchi; Fumio Yoshii; Miura, H.; Murakami, K.

    1996-01-01

    Thus a study has been carried out to investigate the effect of particle to particle variation in crosslink density on physical properties of radiation vulcanized NR latex film. NR latex was irradiated in small bottle by γ rays without vulcanization accelerator to provide latex rubber particles having homogeneous distribution of crosslink density. The doses were 30, 50, 100, 250, 300, 400, 500 and 600 kGy. Weight swelling ratio, gel fraction, tensile strength and elongation at break of the latex film from the mixed latex were measured. The vulcanization dose of this latex was 250 kGy. Then the two different latexes were mixed in a such way to adjust the average dose of 250 kGy to prepare a latex consisting of rubber particles having heterogeneous distribution of crosslink density. Tensile strength of the latex film was depressed by mixing. The reduction increased with increasing the decrease of gel fraction by mixing. However the reduction was not serious when the dose difference of two latexes was less than 200 kGy

  3. Tangent modulus in numerical integration of constitutive relations and its influence on convergence of N-R method

    Directory of Open Access Journals (Sweden)

    Poruba Z.

    2009-06-01

    Full Text Available For the numerical solution of elasto-plastic problems with use of Newton-Raphson method in global equilibrium equation it is necessary to determine the tangent modulus in each integration point. To reach the parabolic convergence of Newton-Raphson method it is convenient to use so called algorithmic tangent modulus which is consistent with used integration scheme. For more simple models for example Chaboche combined hardening model it is possible to determine it in analytical way. In case of more robust macroscopic models it is in many cases necessary to use the approximation approach. This possibility is presented in this contribution for radial return method on Chaboche model. An example solved in software Ansys corresponds to line contact problem with assumption of Coulomb's friction. The study shows at the end that the number of iteration of N-R method is higher in case of continuum tangent modulus and many times higher with use of modified N-R method, initial stiffness method.

  4. Intensity response function of the photopic negative response (PhNR): effect of age and test-retest reliability.

    Science.gov (United States)

    Joshi, Nabin R; Ly, Emma; Viswanathan, Suresh

    2017-08-01

    To assess the effect of age and test-retest reliability of the intensity response function of the full-field photopic negative response (PhNR) in normal healthy human subjects. Full-field electroretinograms (ERGs) were recorded from one eye of 45 subjects, and 39 of these subjects were tested on two separate days with a Diagnosys Espion System (Lowell, MA, USA). The visual stimuli consisted of brief (test-retest reliability was assessed with the Wilcoxon signed-rank test and Bland-Altman analysis. Holm's correction was applied to account for multiple comparisons. V max of BT was significantly smaller than that of PT and b-wave, and the V max of PT and b-wave was not significantly different from each other. The slope parameter n was smallest for BT and the largest for b-wave and the difference between the slopes of all three measures were statistically significant. Small differences observed in the mean values of K for the different measures did not reach statistical significance. The Wilcoxon signed-rank test indicated no significant differences between the two test visits for any of the Naka-Rushton parameters for the three ERG measures, and the Bland-Altman plots indicated that the mean difference between test and retest measurements of the different fit parameters was close to zero and within 6% of the average of the test and retest values of the respective parameters for all three ERG measurements, indicating minimal bias. While the coefficient of reliability (COR, defined as 1.96 times the standard deviation of the test and retest difference) of each fit parameter was more or less comparable across the three ERG measurements, the %COR (COR normalized to the mean test and retest measures) was generally larger for BT compared to both PT and b-wave for each fit parameter. The Naka-Rushton fit parameters did not show statistically significant changes with age for any of the ERG measures when corrections were applied for multiple comparisons. However, the V max of

  5. 17 CFR 279.4 - Form ADV-NR, appointment of agent for service of process by non-resident general partner and non...

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Form ADV-NR, appointment of agent for service of process by non-resident general partner and non-resident managing agent of an... agent for service of process by non-resident general partner and non-resident managing agent of an...

  6. Rho-kinase signaling controls nucleocytoplasmic shuttling of class IIa Histone Deacetylase (HDAC7) and transcriptional activation of orphan nuclear receptor NR4A1

    Energy Technology Data Exchange (ETDEWEB)

    Compagnucci, Claudia; Barresi, Sabina [Unit of Molecular Medicine for Neuromuscular and Neurodegenerative Disorders, Department of Neurosciences, Bambino Gesù Children’s Hospital, IRCCS, Rome (Italy); Petrini, Stefania [Research Laboratories, Confocal Microscopy Core Facility, Bambino Gesù Children’s Hospital, IRCCS, Rome (Italy); Bertini, Enrico [Unit of Molecular Medicine for Neuromuscular and Neurodegenerative Disorders, Department of Neurosciences, Bambino Gesù Children’s Hospital, IRCCS, Rome (Italy); Zanni, Ginevra, E-mail: ginevra.zanni@opbg.net [Unit of Molecular Medicine for Neuromuscular and Neurodegenerative Disorders, Department of Neurosciences, Bambino Gesù Children’s Hospital, IRCCS, Rome (Italy)

    2015-04-03

    Rho-kinase (ROCK) has been well documented to play a key role in RhoA-induced actin remodeling. ROCK activation results in myosin light chain (MLC) phosphorylation either by direct action on MLC kinase (MLCK) or by inhibition of MLC phosphatase (MLCP), modulating actin–myosin contraction. We found that inhibition of the ROCK pathway in induced pluripotent stem cells, leads to nuclear export of HDAC7 and transcriptional activation of the orphan nuclear receptor NR4A1 while in cells with constitutive ROCK hyperactivity due to loss of function of the RhoGTPase activating protein Oligophrenin-1 (OPHN1), the orphan nuclear receptor NR4A1 is downregulated. Our study identify a new target of ROCK signaling via myosin phosphatase subunit (MYPT1) and Histone Deacetylase (HDAC7) at the nuclear level and provide new insights in the cellular functions of ROCK. - Highlights: • ROCK regulates nucleocytoplasmic shuttling of HDAC7 via phosphorylation of MYPT1. • Nuclear export of HDAC7 and upregulation of NR4A1 occurs with low ROCK activity. • High levels of ROCK activity due to OPHN1 loss of function downregulate NR4A1.

  7. Role of a novel dual flavin reductase (NR1) and an associated histidine triad protein (DCS-1) in menadione-induced cytotoxicity

    International Nuclear Information System (INIS)

    Kwasnicka-Crawford, Dorota A.; Vincent, Steven R.

    2005-01-01

    Microsomal cytochrome P450 reductase catalyzes the one-electron transfer from NADPH via FAD and FMN to various electron acceptors, such as cytochrome P450s or to some anti-cancer quinone drugs. This results in generation of free radicals and toxic oxygen metabolites, which can contribute to the cytotoxicity of these compounds. Recently, a cytosolic NADPH-dependent flavin reductase, NR1, has been described which is highly homologous to the microsomal cytochrome P450 reductase. In this study, we show that over-expression of NR1 in human embryonic kidney cells enhances the cytotoxic action of the model quinone, menadione. Furthermore, we show that a novel human histidine triad protein DCS-1, which is expressed together with NR1 in many tissues, can significantly reduce menadione-induced cytotoxicity in these cells. We also show that DCS-1 binds NF1 and directly modulates its activity. These results suggest that NR1 may play a role in carcinogenicity and cell death associated with one-electron reductions

  8. Signal differential-to-noise ratio (SdNR) in the evaluation of radiography techniques for optimisation of the industrial computed radiography

    International Nuclear Information System (INIS)

    Saddock, Aline; Candeias, Janaina P.; Oliveira, Davi F.; Lopes, Ricardo T.

    2007-01-01

    The transition from analog to digital operation which the radiography has gone through, has provided new and important challenges in the way the images are acquired and displayed. There is several acquisition system of digital image, such as for example, the computerized system, which uses Image Plates (IP). This system was used in the accomplishment of this work with the aim to study a technique capable of optimizing the acquisition of digital image. A methodology for the evaluation of image quality is through the parameter signal differential-to- noise ratio (SdNR). However, in order to compare different radiographic techniques through this system it is also necessary to calculate the Figure of Merit (FOM) that in this in case, it is given by the square of the SdNR per unit of applied dose. The method proposed in this work is about the use of IP to carry out SdNR experimental measurements and consequently FOM measurements in applications of the x-ray of pieces in aluminum. This proceeding was performed varying some specific parameters of the system, as high voltage, exposition and the use of filters in the X-rays tube exit. As a result, an SdNR was obtained for each technique, allowing in this way, to verify the behavior of the FOM in each one of them. (author)

  9. Effects of a wide range of dietary nicotinamide riboside (NR) concentrations on metabolic flexibility and white adipose tissue (WAT) of mice fed a mildly obesogenic diet

    NARCIS (Netherlands)

    Shi, W.; Hegeman, M.A.; Dartel, van D.A.M.; Tang, J.; Suarez, M.; Swarts, H.; Hee, van der B.; Arola, L.; Keijer, J.

    2017-01-01

    Scope: Metabolic flexibility is the ability to switch metabolism between carbohydrate oxidation (CHO) and fatty acid oxidation (FAO) and is a biomarker for metabolic health. The effect on metabolic health of nicotinamide riboside (NR) as an exclusive source of vitamin B3 is unknown and is examined

  10. [Effect of electroacupuncture on phosphorylation of NR2B at Tyr 1742 site in the spinal dorsal horn of CFA rats].

    Science.gov (United States)

    Liang, Yi; Fang, Jian-Qiao; Fang, Jun-Fan; Du, Jun-Ying; Qiu, Yu-Jie; Liu, Jin

    2013-10-01

    To observe the effect of electroacupuncture (EA) on phosphorylation of spinal NR2B at Tyr 1742 site in complete Freund's adjuvant (CFA) induced inflammatory pain rats. METHods Forty male Sprague Dawley rats were randomly divided into normal group (N group, n = 10), the model group (CFA group, n = 15), and the EA group (n = 15). The inflammatory pain model was established by subcutaneous injecting CFA (0.1 mL per rat) into the right hind paw. Paw withdrawal thresholds (PWTs) were measured before CFA injection (as the base), as well as at 24 h, 25 h, 3rd day, and 7th day after CFA injection. Phosphorylation of NR2B at Tyr 1742 site in the ispilateral spinal dorsal horn at the 3rd day post-injection were detected using immunohistochemical assay. PWTs in the CFA group were significantly lower than those of the N group at every detective time point post-injection (P CFA group at 25 h and 3rd day post-injection (P CFA group was up-regulated. Compared with the CFA group, the ratio of p-NR2B positive cells in the ispilateral spinal dorsal horn of rats showed a decreasing tendency in the EA group. EA might effectively inhibit CFA-induced inflammatory pain possibly associated with down-regulating phosphorylation of NR2B at Tyr 1742 site in the ispilateral spinal dorsal horn.

  11. The Nuclear Orphan Receptor NR4A1 is Involved in the Apoptotic Pathway Induced by LPS and Simvastatin in RAW 264.7 Macrophages.

    Science.gov (United States)

    Kim, Yong Chan; Song, Seok Bean; Lee, Sang Kyu; Park, Sang Min; Kim, Young Sang

    2014-04-01

    Macrophage death plays a role in several physiological and inflammatory pathologies such as sepsis and arthritis. In our previous work, we showed that simvastatin triggers cell death in LPS-activated RAW 264.7 mouse macrophage cells through both caspase-dependent and independent apoptotic pathways. Here, we show that the nuclear orphan receptor NR4A1 is involved in a caspase-independent apoptotic process induced by LPS and simvastatin. Simvastatin-induced NR4A1 expression in RAW 264.7 macrophages and ectopic expression of a dominant-negative mutant form of NR4A1 effectively suppressed both DNA fragmentation and the disruption of mitochondrial membrane potential (MMP) during LPS- and simvastatin-induced apoptosis. Furthermore, apoptosis was accompanied by Bcl-2-associated X protein (Bax) translocation to the mitochondria. Our findings suggest that NR4A1 expression and mitochondrial translocation of Bax are related to simvastatin-induced apoptosis in LPS-activated RAW 264.7 macrophages.

  12. Quantitative analysis of contents and volatile emissions from a-copaene and quercivorol lures, and longevity for attraction of Euwallacea nr. fornicatus in Florida

    Science.gov (United States)

    Ambrosia beetles in the cryptic species complex Euwallacea nr. fornicatus vector a fungal pathogen responsible for Fusarium dieback, a disease that impacts avocado (Persea americana) and numerous native trees in the USA (California, Florida), Israel, and other countries. Currently, these pests are d...

  13. Effect of fibre treatments on tensile properties of ethylene vinyl acetate/natural rubber/mengkuang leaf fibre (EVA/NR/MLF) thermoplastic elastomer composites

    Science.gov (United States)

    Hashim, Faiezah; Ismail, Hanafi; Rusli, Arjulizan

    2017-07-01

    Nowadays, a great attention has been dedicated to natural fibers as reinforcement for polymer composites. Natural fibers, compared to glass fibers, exhibit better mechanical properties, such as stiffness, impact strength, flexibility and modulus. However, certain drawbacks, such as the incompatibility between fibers and polymer matrices, the tendency to form aggregates during processing and the poor resistance to moisture, reduce the use of these natural fibers as reinforcements in polymers. Several treatments and modifications are being used to improve the adhesion between fibre and matrix. In this work, the effect of bleaching treatments using hydrogen peroxide in the Mengkuang leaf fibre (MLF) was evaluated on tensile properties of Ethylene Vinyl Acetate (EVA)/Natural Rubber (NR)/MLF composites. Treated MLF were mixed with the EVA/NR blend in Haake internal mixer at 120 °C and rotor speed of 50 rpm for 10 minutes. Fibre morphology and the fibre/matrix interface ware further characterized by scanning electron microscopy (SEM). The tensile strength was increased by about 8% as compared to the composites with untreated fibers. The increased adhesion between fiber and matrix was also observed by SEM. Thus, EVA/NR/MLF composites reinforced with the treated fibres exhibited better tensile properties than untreated EVA/NR/MLF composites.

  14. Association of the symbiotic fungi Fusarium euwallaceae, Graphium sp. and Acremonium sp., with the ambrosia beetle Euwallacea nr. fornicatus in avocado

    Science.gov (United States)

    The ambrosia beetle, Euwallacea nr. fornicatus (Coleoptera:Scolytinae), is a new invasive species to Israel. To date, the beetle has been recorded from 48 tree species representing 25 plant families. Amongst the most affected are avocado, castor-bean and box elder. Isolations from beetle heads revea...

  15. Pubertal androgenization and gonadal histology in two 46, XY adolescents with NR5A1 mutations and predominantly female phenotype at birth

    NARCIS (Netherlands)

    M.L. Cools (Martine); P. Hoebeke (Piet); K.P. Wolffenbuttel (Katja); J.A. Stoop (Hans); R. Hersmus (Remko); M. Barbaro (M.); A. Wedell; H.T. Brüggenwirth (Hennie); L.H.J. Looijenga (Leendert); S.L.S. Drop (Stenvert)

    2012-01-01

    textabstractObjective: Most patients with NR5A1 (SF-1) mutations and poor virilization at birth are sex-assigned female and receive early gonadectomy. Although studies in pituitary-specific Sf-1 knockout mice suggest hypogonadotropic hypogonadism, little is known about endocrine function at puberty

  16. High and low nightly running behavior associates with nucleus accumbens N-Methyl-d-aspartate receptor (NMDAR) NR1 subunit expression and NMDAR functional differences.

    Science.gov (United States)

    Grigsby, Kolter B; Kovarik, Cathleen M; Rottinghaus, George E; Booth, Frank W

    2018-04-03

    The extent to which N-Methyl-d-aspartate (NMDA) receptors facilitate the motivation to voluntarily wheel-run in rodents has yet to be determined. In so, we utilized female Wistar rats selectively bred to voluntarily run high (HVR) and low (LVR) nightly distances in order to examine if endogenous differences in nucleus accumbens (NAc) NMDA receptor expression and function underlies the propensity for high or low motivation to voluntarily wheel-run. 12-14 week old HVR and LVR females were used to examine: 1.) NAc mRNA and protein expression of NMDA subunits NR1, NR2A and NR2B; 2.) NMDA current responses in isolated medium spiny neurons (MSNs) and 3.) NMDA-evoked dopamine release in an ex vivo preparation of NAc punches. Expectedly, there was a large divergence in nightly running distance and time between HVR and LVR rats. We saw a significantly higher mRNA and protein expression of NR1 in HVR compared to LVR rats, while seeing no difference in the expression of NR2A or NR2B. There was a greater current response to a 500 ms application of 300 μM of NMDA in medium-spiny neurons isolated from the NAc HVR compared to LVR animals. On average, NMDA-evoked punches (50 μM of NMDA for 10 min) taken from HVR rats retained ∼54% of the dopamine content compared to their bilateral non-evoked sides, while evoked punches from LVR animals showed no statistical decrease in dopamine content compared to their non-evoked sides. Collectively, these data suggest a potential link between NAc NR1 subunit expression as well as NMDA function and the predisposition for nightly voluntary running behavior in rats. In light of the epidemic rise in physical inactivity, these findings have the potential to explain a neuro-molecular mechanism that regulates the motivation to be physically active. Copyright © 2018 Elsevier B.V. All rights reserved.

  17. Elevated utero/placental GR/NR3C1 is not required for the induction of parturition in the dog.

    Science.gov (United States)

    Gram, Aykut; Trachsel, Alexandra; Boos, Alois; Kowalewski, Mariusz P

    2016-10-01

    The endocrine mechanisms that lead to initiation of parturition in dogs are still not fully understood. The prepartum luteolysis is associated with increased prostaglandin (PG) F2α secretion; however, there is no pregnancy- or parturition-related increase in estrogens. Moreover, unlike in other mammalian species, in the dog, increased peripartum levels of cortisol measured sporadically in maternal peripheral blood are not mandatory for normal parturition. Nevertheless, auto/paracrine effects of cortisol at the placental feto-maternal level cannot be excluded. Therefore, the aim of this study was to investigate the expression and localization of glucocorticoid receptor (GR/NR3C1) in canine utero/placental (Ut/Pl) units and uterine interplacental sites at selected time points during pregnancy (pre-implantation, post-implantation and mid-gestation), and at normal and antigestagen-induced parturition. The Ut/Pl expression of GR/NR3C1 did not change significantly from pre-implantation until mid-gestation; however, it was strongly induced during the prepartum luteolysis. Within the interplacental samples, expression of GR/NR3C1-mRNA was greater post-implantation than pre-implantation and did not change afterward, i.e. toward mid-gestation. Compartmentalization studies within the Ut/Pl units, involving placenta, endometrium and myometrium separately, performed at the prepartum luteolysis revealed the highest GR/NR3C1-mRNA levels in placenta compared with endometrium and myometrium. Interestingly, in antigestagen-treated mid-pregnancy dogs, Ut/Pl and interplacental GR/NR3C1-mRNA expression remained unaffected. At the cellular level, placental GR/NR3C1 was clearly detectable in placenta fetalis, i.e. in trophoblast cells. In conclusion, increased expression of GR/NR3C1 during normal parturition, but not following antigestagen-treatment, suggest that it is not required for initiating the signaling cascade of PG synthesis leading to the induction of parturition in the dog.

  18. The ROS/NF-κB/NR4A2 pathway is involved in H2O2 induced apoptosis of resident cardiac stem cells via autophagy.

    Science.gov (United States)

    Shi, Xingxing; Li, Wenjing; Liu, Honghong; Yin, Deling; Zhao, Jing

    2017-09-29

    Cardiac stem cells (CSCs)-based therapy provides a promising avenue for the management of ischemic heart diseases. However, engrafted CSCs are subjected to acute cell apoptosis in the ischemic microenvironment. Here, stem cell antigen 1 positive (Sca-1 + ) CSCs proved to own therapy potential were cultured and treated with H 2 O 2 to mimic the ischemia situation. As autophagy inhibitor, 3-methyladenine (3MA), inhibited H 2 O 2 -induced CSCs apoptosis, thus we demonstrated that H 2 O 2 induced autophagy-dependent apoptosis in CSCs, and continued to find key proteins responsible for the crosstalk between autophagy and apoptosis. Nuclear Receptor Subfamily 4 Group A Member 2 (NR4A2), increased upon cardiomyocyte injury with unknown functions in CSCs, was increased by H 2 O 2 . NR4A2 siRNA attenuated H 2 O 2 induced autophagy and apoptosis in CSCs, which suggested an important role of NR4A2 in CSCs survival in ischemia conditions. Reactive oxygen species (ROS) and NF-κB (P65) subunit were both increased by H 2 O 2 . Either the ROS scavenger, N-acetyl-l-cysteine (NAC) or NF-κB signaling inhibitor, bay11-7082 could attenuate H 2 O 2 -induced autophagy and apoptosis in CSCs, which suggested they were involved in this process. Furthermore, NAC inhibited NF-κB activities, while bay11-7082 inhibited NR4A2 expression, which revealed a ROS/NF-κB/NR4A2 pathway responsible for H 2 O 2 -induced autophagy and apoptosis in CSCs. Our study supports a new clue enhancing the survival rate of CSCs in the infarcted myocardium for cell therapy in ischemic cardiomyopathy.

  19. H2O2 attenuates IGF-1R tyrosine phosphorylation and its survival signaling properties in neuronal cells via NR2B containing NMDA receptor.

    Science.gov (United States)

    Zeng, Zhiwen; Wang, Dejun; Gaur, Uma; Rifang, Liao; Wang, Haitao; Zheng, Wenhua

    2017-09-12

    Impairment of insulin-like growth factor I (IGF-I) signaling plays an important role in the development of neurodegeneration. In the present study, we investigated the effect of H 2 O 2 on the survival signaling of IGF-1 and its underlying mechanisms in human neuronal cells SH-SY5Y. Our results showed that IGF-1 promoted cell survival and stimulated phosphorylation of IGF-1R as well as its downstream targets like AKT and ERK1/2 in these cells. Meanwhile, these effects of IGF-1 were abolished by H 2 O 2 at 200μM concentration which did not cause any significant toxicity to cells itself in our experiments. Moreover, studies using various glutamate receptor subtype antagonists displayed that N-methyl-D -aspartate (NMDA) receptor antagonist dizocilpine maleate (MK-801) blocked the effects of H 2 O 2 , whereas other glutamate receptor subtype antagonists, such as non-NMDA receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX), metabolic glutamate receptor antagonists LY341495 and CPCCOEt, had no effect. Further studies revealed that NR2B-containing NMDARs are responsible for these effects as its effects were blocked by pharmacological inhibitor Ro25-698 or specific siRNA for NR2B, but not NR2A. Finally, our data also showed that Ca 2+ influx contributes to the effects of H 2 O 2 . Similar results were obtained in primary cultured cortical neurons. Taken together, the results from the present study suggested that H 2 O 2 attenuated IGF-1R tyrosine phosphorylation and its survival signaling properties via NR2B containing NMDA receptors and Ca 2+ influx in SH-SY5Y cells. Therefore, NMDAR antagonists, especially NR2B-selective ones, combined with IGF-1 may serve as an alternative therapeutic agent for oxidative stress related neurodegenerative disease.

  20. Diagnosis of extraskeletal myxoid chondrosarcoma in the thigh using EWSR1-NR4A3 gene fusion: a case report.

    Science.gov (United States)

    Kobayashi, Hiroki; Kikuta, Kazutaka; Sekita, Tetsuya; Susa, Michiro; Nishimoto, Kazumasa; Sasaki, Aya; Kameyama, Kaori; Sugita, Shintaro; Hasegawa, Tadashi; Nakamura, Masaya; Matsumoto, Morio; Morioka, Hideo

    2016-11-10

    Extraskeletal myxoid chondrosarcoma is a rare soft tissue sarcoma that has unusual ultrastructural and molecular features. However, unlike other soft tissue sarcomas, it does not have specific clinical symptoms or radiological features, which can make its diagnosis difficult. Nevertheless, extraskeletal myxoid chondrosarcoma has a rare gene fusion (EWSR1-NR4A3) that is useful for making a differential diagnosis. A 43-year-old Japanese man presented with a soft tissue mass in his right thigh. A physical examination and radiography revealed a large soft tissue mass. During magnetic resonance imaging, the mass exhibited isointensity on T1-weighted images and high intensity on T2-weighted images, as well as gadolinium enhancement at the side edge of the partition structure. Thus, we considered a possible diagnosis of a malignant myxoid soft tissue tumor, such as myxoid liposarcoma, myxofibrosarcoma, or metastatic carcinomas, including myoepithelial tumor and neuroendocrine tumor, and performed an incisional biopsy to make a definitive diagnosis. The pathological findings revealed a lobulated tumor with a myxoid structure and atypical spindle-shaped cells that created eosinophilic cord-like forms. Immunohistochemistry revealed that the tumor was positive for S-100 and negative for synaptophysin, chromogranin A, and pan keratin (AE1/AE3). The percentage of Ki-67 was 10 % in the hot spot area. Based on these clinicopathological findings, we initially considered the possibility of a myxoid liposarcoma, although we did not observe any lipoblasts. Therefore, we considered the possibility of an extraskeletal myxoid chondrosarcoma. As this tumor is very rare, we searched for the EWSR1-NR4A3 gene fusion using fluorescence in situ hybridization, which confirmed the diagnosis of extraskeletal myxoid chondrosarcoma. Positron emission tomography-computed tomography did not identify any obvious metastases, and we performed radical resection of our patient's vastus medialis and

  1. Opinion presented on the behalf of the Commission for Sustainable Development and Land Planning on the 2015 on the finance bill project for 2015 (nr 2234), Volume I - Ecology, sustainable development and mobility, risk prevention - Meteorology - Nr 2266

    International Nuclear Information System (INIS)

    Krabal, Jacques

    2014-01-01

    The first part of this report discusses the credits asked for 2015 for the 'Risk prevention' program (program nr 181) in different areas: prevention and struggle in the field of natural and hydraulic risks (fund for the prevention of major natural risks, prevention of flooding and safety of dams and dikes), prevention and struggle in the field of technological risks (prevention plans, nuclear safety and radiation protection), public health and environment policies (evolution of the PNSE, struggle against noise, waste management). The second part discusses the credits asked for 2015 for the 'Meteorology' program: program structure and asked credits, position of the market of meteorological information services, implementation of the contract for objectives and performance and achievement of a denser territorial coverage

  2. Particle Reduction Strategies - PAREST. A meteorology comparision for better understanding of different pollutant concentration simulations with different chemistry transport models. Sub-report; Strategien zur Verminderung der Feinstaubbelastung - PAREST. Ein Meteorologievergleich zum besseren Verstaendnis der unterschiedlichen Schadstoffkonzentrationssimulationen mit verschiedenen Chemie-Transport-Modellen. Teilbericht

    Energy Technology Data Exchange (ETDEWEB)

    Kerschbaumer, Andreas [Freie Univ. Berlin (Germany). Inst. fuer Meteorologie, Troposphaerische Umweltforschung

    2013-06-15

    In this study, input variables for chemical transport models were compared from different meteorological drivers each with other and with the measured friction velocities in one place. Friction velocities are used as a measure of the mechanical turbulence, but are made available neither by numerical weather models nor routinely available observations: so they must either recalculate or estimated. In this study, friction velocities (u{sup *}), which is measured for years by the DWD in Lindenberg near Berlin, averaged over 30 minutes and have been made available to FU Berlin, compared with modeled values. The considered models are firstly the diagnostic interpolation model TRAMPER, on the other the prognostic models of the DWD COSMO-EU and the EZMWF. The boundary layer relevant parameters from the NWM were recalculated from the involved groups, which has been made for the COSMO-EU the one from the FU Berlin, the other from IfT in Leipzig. The boundary layer variables in the EZMWF model, however, were determined by TNO Netherlands. The measurements have shown that u{sup *} in the forest twice as high as above the meadow. [German] In dieser Studie wurden Eingangsvariablen fuer Chemie-Transport-Modelle aus verschiedenen meteorologischen Treibern miteinander und mit an einem Ort gemessenen Schubspannungsgeschwindigkeiten verglichen. Schubspannungsgeschwindigkeiten werden als Mass fuer die mechanische Turbulenz benutzt, werden aber weder von numerischen Wettermodellen noch routinemaessig von Beobachtungen zur Verfuegung gestellt: sie muessen also entweder nachgerechnet oder abgeschaetzt werden. In dieser Arbeit wurden Schubspannungsgeschwindigkeiten (u{sup *}), die vom DWD in Lindenberg bei Berlin ueber Jahre gemessen, gemittelt ueber 30 Minuten, und der FUBerlin zur Verfuegung gestellt worden sind, mit modellierten Werten verglichen. Die beruecksichtigten Modelle sind zum einen das diagnostische Interpolationsmodell TRAMPER, zum anderen die prognostischen Modelle des DWD

  3. Informal Institutions and the Weaknesses of Human Behavior

    Science.gov (United States)

    2005-01-01

    Volkswirtschaftslehre "* Braiuninger, Michael, Social Capital and Regional Mobility, Nr. 4/2002. "* Schdfer, Wolf, EU-Erweiterung: Anmerkungen zum Balassa ... Samuelson -Effekt, Nr. 3/2002, erschienen in: Stefan Reitz (Hg.): Theoretische und wirtschaftspolitische Aspekte der internatio- nalen Integration, Duncker...646-660. Friuhere Diskussionsbeitraige zur Finanzwissenschaft "* Josten, Stefan, Crime, Inequality, and Economic Growth. A Classical Argument for

  4. Nuclear receptor 4a3 (nr4a3 regulates murine mast cell responses and granule content.

    Directory of Open Access Journals (Sweden)

    Gianni Garcia-Faroldi

    Full Text Available Nuclear receptor 4a3 (Nr4a3 is a transcription factor implicated in various settings such as vascular biology and inflammation. We have recently shown that mast cells dramatically upregulate Nuclear receptor 4a3 upon activation, and here we investigated the functional impact of Nuclear receptor 4a3 on mast cell responses. We show that Nuclear receptor 4a3 is involved in the regulation of cytokine/chemokine secretion in mast cells following activation via the high affinity IgE receptor. Moreover, Nuclear receptor 4a3 negatively affects the transcript and protein levels of mast cell tryptase as well as the mast cell's responsiveness to allergen. Together, these findings identify Nuclear receptor 4a3 as a novel regulator of mast cell function.

  5. Effects of a NR2B Selective NMDA Glutamate Antagonist, CP-101,606, on Dyskinesia and Parkinsonism

    Science.gov (United States)

    Nutt, John G.; Gunzler, Steven A; Kirchhoff, Trish; Hogarth, Penelope; Weaver, Jerry L.; Krams, Michael; Jamerson, Brenda; Menniti, Frank S.; Landen, Jaren W.

    2011-01-01

    Glutamate antagonists decrease dyskinesia and augment the antiparkinsonian effects of levodopa in animal models of Parkinson’s disease (PD). In a randomized, double-blind, placebo-controlled clinical trial we investigated the acute effects of placebo and two doses of a NR2B subunit selective NMDA glutamate antagonist, CP-101,606, on the response to two-hour levodopa infusions in 12 PD subjects with motor fluctuations and dyskinesia. Both doses of CP-101,606 reduced the maximum severity of levodopa-induced dyskinesia approximately 30% but neither dose improved parkinsonism. CP-101,606 was associated with a dose-related dissociation and amnesia. These results support the hypothesis that glutamate antagonists may be useful antidyskinetic agents. However, future studies will have to determine if the benefits of dyskinesia suppression can be achieved without adverse cognitive effects. PMID:18759356

  6. Deficiency of the NR4A Orphan Nuclear Receptor NOR1 attenuates Neointima Formation Following Vascular Injury

    Science.gov (United States)

    Nomiyama, Takashi; Zhao, Yue; Gizard, Florence; Findeisen, Hannes M.; Heywood, Elizabeth B.; Jones, Karrie L.; Conneely, Orla M.; Bruemmer, Dennis

    2009-01-01

    Background The neuron-derived orphan receptor-1 (NOR1) belongs to the evolutionary highly conserved and most ancient NR4A subfamily of the nuclear hormone receptor superfamily. Members of this subfamily function as early response genes regulating key cellular processes including proliferation, differentiation, and survival. Although NOR1 has previously been demonstrated to be required for smooth muscle cell (SMC) proliferation in vitro, the role of this nuclear receptor for the proliferative response underlying neointima formation and target genes trans-activated by NOR1 remain to be defined. Methods and Results Using a model of guide wire-induced arterial injury, we demonstrate decreased neointima formation in NOR1-/- mice compared to wildtype mice. In vitro, NOR1-deficient SMC exhibit decreased proliferation due to a G1→S phase arrest of the cell cycle and increased apoptosis in response to serum deprivation. NOR1-deficiency alters phosphorylation of the retinoblastoma protein by preventing mitogen-induced cyclin D1 and D2 expression. Conversely, overexpression of NOR1 induces cyclin D1 expression and the transcriptional activity of the cyclin D1 promoter in transient reporter assays. Gel shift and chromatin immunoprecipitation assays identified a putative response element for NR4A receptors in the cyclin D1 promoter, to which NOR1 is recruited in response to mitogenic stimulation. Finally, we provide evidence that these observations are applicable in vivo by demonstrating decreased cyclin D1 expression during neointima formation in NOR1-deficient mice. Conclusions These experiments characterize cyclin D1 as a NOR1-regulated target gene in SMC and demonstrate that NOR1 deficiency decreases neointima formation in response to vascular injury. PMID:19153266

  7. Investigations on radionuclide release and on the corrosion behaviour of spent fuels from research reactors under disposal conditions. Final report; Untersuchungen zur Radionuklidfreisetzung und zum Korrosionsverhalten von bestrahltem Kernbrennstoff aus Forschungsreaktoren unter Endlagerbeedingungen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Bruecher, H.; Curtius, H.; Fachinger, J.; Kaiser, G.; Mazeina, L.; Nau, K.

    2003-12-01

    From the present report 'Untersuchungen zur Radionuklidfreisetzung und zum Korrosionsverhalten von bestrahltem Kernbrennstoff aus Forschungsreaktoren unter Endlagerbedingungen' with the code number FKZ 9108 carried out in the time periode 01.06.1998 till 30.11.2001 the following results can be withdrawn: U/Al-RR-fuel elements corroded slowly in granite water (Grimsel-West) at 90 C under anaerobic conditions. For a complete dissolution of the fuel element a time period of 10{sup 3} years is assumed according to present conservative results. In salt brines, especially in magnesium chloride rich brines the corrosion rate is high. Addition of GGG40 (basic material of the fuel element container with iron as main element) had an acceleration effect. A complete dissolution of the fuel is achieved within a couple of months. Under aerobic and under anaerobic conditions the bulk of released radionuclides were fixed by the corrosion products formed (secondary phases). The actinides were mobilised by variation of the ionic strength of the leaching solution. This process can be explained by phase conversion reactions within the secondary phases. Secondary phases formed by corrosion of a non-irradiated U/Al-RR-fuel element, were analysed and hydrotalcites were identified as phase components. This result justifies the assumption, that hydrotalcites are components of the corrosion products from irradiated fuels. To clarify the questions which bindings exist between radionuclides and secondary phases, sorption experiments were performed. The sorption experiments were performed in salt brines and in granite water using repository relevant radionuclides and minerals which are considered to represent thermodynamic final components of the secondary phases. Pu sorbed as cationic species quantitatively and the binding is covalent. In granite water the same behaviour was found for Am. (orig.) [German] Aus dem vorliegenden Bericht 'Untersuchungen zur Radionuklidfreisetzung

  8. Reduction of radiation dose by using digital luminescence radiography compared to conventional screen film system with grid cassette; Reduktion der Strahlendosis mittels Speicherfolienradiographie im Vergleich zum konventionellen Film-Folien-System mit Rasterkassette am Schaedelphantom

    Energy Technology Data Exchange (ETDEWEB)

    Heyne, J.P.; Merbold, H.; Neumann, R.; Freesmeyer, M.; Jonetz-Mentzel, L.; Kaiser, W.A. [Friedrich-Schiller-Univ. Jena, Inst. fuer Diagnostische und Interventionelle Radiologie (Germany); Sehner, J. [AGFA-Deutschland, Vertriebsgesellschaft (Germany)

    1999-07-01

    Purpose: How much can the radiation dose be reduced for skull radiography by using digital luminescence radiography (DLR) compared to a conventional screen film system with a grid cassette? Methods and Materials: A skull phantom (3M) was X-rayed in anterior-posterior orientation using both a conventional screen film system with grid cassette and DLR (ADC-70, Agfa). The tube current time product (mAs) was diminished gradually while keeping the voltage constant. The surface entrance dose was measured by a sensor of Dosimax (Wellhoefer). Five investigators evaluated the images by characteristic and critical features, spatial resolution and contrast. Results: The surface entrance dose at 73 kV/22 mAs was 0,432 mGy in conventional screen film system and 0,435 mGy in DLR. The images could be evaluated very well down to an average dose of 71% (0,308 mGy; SD 0,050); sufficient images were obtained down to an average dose of 31% (0,136 mGy; SD 0,065). The resolution of the line pairs were reduced down to a 2 levels depending on the investigator. Contrast was assessed as being very good to sufficient. The acceptance of the postprocessed images (MUSICA-software) was individually different and resultde in an improvement of the assessment of bone structures an contrast in higher dose ranges only. Conclusion: For the sufficient assessment of a possible fracture/of paranasal sinuses/of measurement the skull the dose can be reduced to at least 56% (31%; SD 14,9%)/40% (27%; SD 9,3%)/18% (14%; SD 4,4%). Digital radiography allows question-referred exposure parameters with clearly reduced dose, so e.g. for fracture exclusion 73 kV/12,5 mAs and to skull measurement 73 kV/4 mAs. (orig.) [Deutsch] Ziel: Wie weit kann unter Einsatz der rasterlosen Speicherfolienradiographie bei einer Schaedelaufnahme die Strahlendosis im Vergleich zum Film-Folien-System (FFS) mit Rasterkassette (RK) fragestellungsbezogen gesenkt werden? Material und Methode: Ein Schaedelphantom (3M) wurde konventionell

  9. Radiation protection in medical research. Licensing requirement for the use of radiation and advice for the application procedure; Strahlenschutz in der medizinischen Forschung. Genehmigungsbeduerftigkeit von Strahlenanwendungen und Hinweise zum Antragsverfahren

    Energy Technology Data Exchange (ETDEWEB)

    Minkov, V.; Klammer, H.; Brix, G. [Bundesamt fuer Strahlenschutz, Abteilung fuer medizinischen und beruflichen Strahlenschutz, Neuherberg (Germany)

    2017-07-15

    In Germany, persons who are to be exposed to radiation for medical research purposes are protected by a licensing requirement. However, there are considerable uncertainties on the part of the applicants as to whether licensing by the competent Federal Office for Radiation Protection is necessary, and regarding the choice of application procedure. The article provides explanatory notes and practical assistance for applicants and an outlook on the forthcoming new regulations concerning the law on radiation protection of persons in the field of medical research. Questions and typical mistakes in the application process were identified and evaluated. The qualified physicians involved in a study are responsible for deciding whether a license is required for the intended application of radiation. The decision can be guided by answering the key question whether the study participants would undergo the same exposures regarding type and extent if they had not taken part in the study. When physicians are still unsure about their decision, they can seek the advisory service provided by the professional medical societies. Certain groups of people are particularly protected through the prohibition or restriction of radiation exposure. A simplified licensing procedure is used for a proportion of diagnostic procedures involving radiation when all related requirements are met; otherwise, the regular licensing procedure should be used. The new radiation protection law, which will enter into force on the 31st of december 2018, provides a notification procedure in addition to deadlines for both the notification and the licensing procedures. In the article, the authors consider how eligible studies involving applications of radiation that are legally not admissible at present may be feasible in the future, while still ensuring a high protection level for study participants. (orig.) [German] Personen, bei denen Strahlenanwendungen zum Zweck der medizinischen Forschung durchgefuehrt

  10. Is tamoxifen associated with an increased risk for thromboembolic complications in patients undergoing microvascular breast reconstruction? [Ist die Einnahme von Tamoxifen zum Zeitpunkt der mikrovaskulären Brustrekonstruktion mit einer erhöhten Rate an thrombembolischen Komplikationen assoziiert?

    Directory of Open Access Journals (Sweden)

    Krämer, Robert

    2013-02-01

    des hormonrezeptorpen Brustkrebses postmenopausaler Frauen abgelöst. Tamoxifen ist mit einem ca. zweifach erhöhten Risiko für thrombembolische Komplikationen (tiefe Beinvenenthrombose, Lungenembolie assoziiert. Vor dem Hintergrund einer aktuellen und im Februar 2012 im Journal of Plastic and Reconstructive Surgery erschienenen retrospektiven Studie mit Nachweis einer erhöhten Komplikationsrate freier Lappentransplantate zur Brustrekonstruktion unter Tamoxifen-Einnahme haben wir unser Patientenkollektiv nach mikrochirurgischer Brustrekonstruktion retrospektiv auf Minor- und Major-Komplikationen einschließlich thrombembolischer Ereignisse insbesondere vor dem Hintergrund einer perioperativen Tamoxifeneinnahme untersucht. Eingeschlossen wurden insgesamt 29 Patientinnen nach mikrochirurgischer autologer Brustrekonstruktion. Es wurden mikrovaskuläre Lappenkomplikationen und Lappenverluste von Patientinnen ohne und mit Einnahme von Tamoxifen und Aromatasehemmern zum Zeitpunkt der mikrochirurgischen Brustrekonstruktion verglichen. 25 Patientinnen (86,2% erhielten eine DIEP-Lappenplastik und 4 (13,8% eine TRAM-Lappenplastik. Fernerhin verglichen wir die Rate allgemeiner thrombembolischer Ereignisse unter Berücksichtigung sonstiger Nebendiagnosen und Risikofaktoren. Von insgesamt 29 Patientinnen standen zum Zeitpunkt der mikrovaskulären Brustrekonstruktion 3 unter einer Aromatasehemmer-Therapie und 5 unter einer Tamoxifen-Therapie. Insgesamt traten in 11 Fällen (37,9% postoperative Komplikationen auf, in 5 Fällen handelte es sich hierbei um Major-Komplikationen (mit einem Lappenverlust und in um Minorkomplikationen. Unter Tamoxifeneinnahme kam es in einem Fall zu einer Minorkomplikation, und in einem anderen Fall zu einem thrombembolisch bedingten Lappenverlust. Insgesamt kam es postoperativ zu einem thrombembolischen Ereignis in Form einer Lungenembolie ohne Assoziation zur Tamoxifen-Therapie.Die Anzahl thrombembolischer Ereignisse stellten sich mit und ohne

  11. Environment-friendly building. Energy efficiency - thermal comfort - materials. Publication on the occasion of the 60th birthday of Gerd Hauser; Umweltbewusstes Bauen. Energieeffizienz - Behaglichkeit - Materialien. Festschrift zum 60. Geburtstag von Gerd Hauser

    Energy Technology Data Exchange (ETDEWEB)

    Maas, A. (ed.)

    2008-07-01

    In honour of the 60th birthday of Professor Gerd Hauser, ''father of the Energy Passport'' in this festival script 38 contributions are compiled in recognition of his work. These articles present the whole thematics of heat- and energy transfer in the building sector. The broad spectra is arranged into the sections energy efficiency, thermal comfort and materials. Constructive problems on sun protection, computer-simulated models of indoor climate, energetic sanitation of monuments taking into account preservative aspects, problems of sound emission from traffic as the constructional physical evaluation of materials in the building envelope. Furtheron the actual discussion on energy saving construction is reflected due to perspective and strategies of climate policy in Germany. The spectrum of the contribution from research and practice shows clearly, that construction physics has developed since last decades from a niche position to a comprehensive branche. (orig./GL) [German] Aus Anlass des 60. Geburtstages von Professor Gerd Hauser, dem Vater des Energiepasses, sind in dieser Festschrift in Anerkennung seines Werkes 38 Beitraege zusammengestellt. Diese stellen die gesamte Thematik der Waerme- und Energieuebertragung im Gebaeudebereich vor. Das breite Spektrum ist in die Abschnitte Energieeffizienz, Behaglichkeit und Materialien gegliedert. Behandelt werden beispielsweise baukonstruktive Fragestellungen beim Sonnenschutz, Computer simulierte Raumklimamodelle, denkmalpflegerische Aspekte bei der energetischen Sanierung, Probleme der Schalluebertragung im Verkehr sowie die bauphysikalische Bewertung von Materialien der Gebaeudehuelle. Darueber hinaus wird die aktuelle Diskussion zum Energiesparenden Bauen im Hinblick auf die Perspektiven und Strategien der Klimapolitik in Deutschland wiedergegeben. Die Bandbreite der Beitraege aus Forschung und Praxis zeigen anschaulich, dass sich die Bauphysik in den letzten Jahrzehnten aus einer

  12. The Eckert number phenomenon - experimental investigations of heat transfer on moving walls, e.g. rotating cylinders; Das Eckert-Zahl-Phaenomen - Experimentelle Untersuchungen zum Waermeuebergang an einer bewegten Wand am Modellfall eines rotierenden Zylinders

    Energy Technology Data Exchange (ETDEWEB)

    Gschwendtner, M.

    2000-07-01

    The Eckert number phenomenon - theoretically investigated by Geropp in 1969 - describes a turnover in heat transfer at a moving wall at an Eckert number EC=1. This report is the first to confirm the Eckert number phenomenon experimentally. Heat transfer on a heated, vertically rotating cylinder in a crossflow was investigated at extreme rotational speeds, i.e. in the range where the predicted phenomenon will occur. A heating concept had to be developed which allowed an input of heating power independent of rotational speed and which therefore had to be contact-free. The complex thermofluiddynamic processes in the boundary layer around the rotating cylinder were investigated and measured using predominantly optical measuring techniques. The results show that the temperature difference between the wall and the surrounding fluid had a significant effect on the predicted turnover of heat transfer at the wall. Moreover, maximum heat transfer occurs at an Eckert number Ec=0.3, which is of great importance for the cooling of hot surfaces in an airstream. [German] Das Eckert-Zahl-Phaenomen - von Geropp 1969 theoretisch untersucht - beschreibt den Umschlag des Waermeueberganges an einer bewegten Wand bei einer Eckert-ZahlEc{approx}1. In der vorliegenden Arbeit wird das Eckert-Zahl-Phaenomen zum ersten Mal experimentell bestaetigt. Dazu wurde der Waermeuebergang am Modellfall eines queransgestroemten, beheizten, vertikalrotierenden Zylinders untersucht. Aufgrund der fuer die Experimente notwendigen extremen Drehzahlen musste fuer die Zylinderheizung ein Konzept entwickelt werden, das eine beruehrungsfreie und damit drehzahlunabhaengige Leistungseinspeisung erlaubte. Mit vorwiegend optischen Messmethoden wurden die komplexen thermofluiddynamischen Vorgaenge in der Grenzschicht um den rotierenden Zylinder untersucht und vermessen. Die Ergebnisse zeigen u.a., dass die Temperaturdifferenz zwischen Wand und Umgebung von entscheidender Bedeutung fuer die Richtungsumkehr des

  13. Newly developed active braze powders based on commercial nickel brazes using zirconium as active element for joining ceramic to metal; Entwicklung von neuen Aktivlotpulvern auf Basis kommerzieller Nickellote mit Zirkon als aktivelement zum Fuegen von Keramik-Metall-Verbunden

    Energy Technology Data Exchange (ETDEWEB)

    Bobzin, K.; Schlaefer, T.; Kopp, N.; Schlegel, A. [Institut fuer Oberflaechentechnik der RWTH Aachen (Germany)

    2010-06-15

    10 weight-%. For the characterisation, the melting range of all experimental brazing filler metals has been determined by thermoanalytical methods and the ones, which have been manufactured by powder metallurgy, have been investigated by means of an x-ray diffraction analysis and a morphological analysis of the powder particles. The suitability of the experimental brazing filler metals for the active brazing has been probed in wetting tests on alumina in high-vacuum brazing trials and examined metallographically. (Abstract Copyright [2010], Wiley Periodicals, Inc.) [German] Die gestiegenen Anforderungen an hochbeanspruchte Bauteilkomponenten hinsichtlich Bestaendigkeit gegen thermische, oxidierende und korrosive Beanspruchungen, Haerte sowie Verschleissbestaendigkeit praedestinieren den Einsatz von ingenieurkeramischen Werkstoffen. Dem gegenueber stehen Eigenschaften wie hohe Sproedigkeit, zum Teil geringe Thermoschockbestaendigkeit, schwierige Bearbeitbarkeit und daraus resultierende Einschraenkungen bei der konstruktiven Gestaltung. Hybride Werkstoffkonzepte als Kombination ingenieurkeramischer Werkstoffe mit metallischen Konstruktionswerkstoffen koennen, sofern dazu geeignete und leistungsfaehige Fuegetechniken verfuegbar sind, technologisch interessante Loesungen darstellen. Das Aktivloeten als eine in der Werkstoffauswahl sehr flexible Fuegetechnologie bietet sich fuer die Erschliessung neuer und innovativer Anwendungen wie beispielsweise der Hochtemperatur-Brennstoffzellen-Technik an. Derzeit in der industriellen Praxis eingesetzte Aktivlote auf Silber/Kupfer-, Kupfer- und Silberbasis weisen bei etwa 500 C einen deutlichen Abfall bezueglich ihrer mechanischen Festigkeit auf. Gold- oder palladiumhaltige Aktivlote mit diesbezueglich besseren Eigenschaften finden aufgrund ihres hohen Preises aeusserst selten Verwendung. Die hier vorgestellten Untersuchungen haben das Ziel, neue Aktivlotlegierungen bei vertretbaren Rohstoffkosten fuer Einsatztemperaturen oberhalb

  14. SPECT/CT for staging and treatment monitoring in oncology. Applications in differentiated thyroid cancer and liver tumors; SPECT/CT zum initialen Staging und Therapiemonitoring in der Onkologie. Indikationen beim differenzierten Schilddruesenkarzinom und bei Lebertumoren

    Energy Technology Data Exchange (ETDEWEB)

    Weber, K.; Berger, F.; Reiser, M.F. [Klinikum der Ludwig-Maximilians-Universitaet Muenchen, Campus Innenstadt, Institut fuer Klinische Radiologie, Muenchen (Germany); Mustafa, M.; Bartenstein, P.; Haug, A. [Klinikum der Ludwig-Maximilians-Universitaet Muenchen, Campus Grosshadern, Klinik und Poliklinik fuer Nuklearmedizin, Muenchen (Germany)

    2012-07-15

    and SPECT/CT provides more accurate imaging of the arterial supply of the liver and of potential outflows of micro-spheres into other organs. SPECT/CT allows evaluation and quantification of the uptake of liver tumors. Improved postablative staging in patients with differentiated thyroid cancer by SPECT/CT in comparison to radioiodine whole body scans can be achieved. Improved planning and monitoring of SIRT therapies utilizing SPECT/CT leads to optimized therapeutic doses within liver lesions. Integration of SPECT/CT into the clinical standard for postablative staging in patients with DTC is recommended as well as utilization of SPECT/CT during the planning process, for dose calculation and treatment monitoring of SIRT therapies. (orig.) [German] In den letzten Jahren hat die Hybridbildgebung mit Verbindung von funktioneller und morphologischer Information zur Diagnostik zahlreicher Erkrankungen zunehmend an Bedeutung gewonnen. Bei Patienten mit differenziertem Schilddruesenkarzinom (DTC) wird nach erfolgter Radiojodtherapie (RJT) ein planares Ganzkoerperszintigramm zum Staging durchgefuehrt. Die diagnostische Genauigkeit dieser szintigraphischen Methode ist jedoch aufgrund limitierter raeumlicher Aufloesung begrenzt. Die Radioembolisation von mit {sup 90}Yttrium beladenen Mikrosphaeren (selektive interne Radiotherapie, SIRT) ermoeglicht eine wenig invasive Therapie primaerer und sekundaerer Lebertumoren. Zur Vermeidung von Nebenwirkungen der Mikrosphaeren durch einen Abstrom in Darm, Magen und Lunge muss vor Therapiebeginn eine Darstellung des durch die leberversorgenden Arterien versorgten Gebiets mittels {sup 99m}Tc-MAA ({sup 99m}Technetium-makroaggregiertes Albumin) und einer Szintigraphie erfolgen. Auch hier limitiert die begrenzte morphologische Information der Szintigraphie das Therapiemonitoring. {sup 131}Jod-Ganzkoerperszintigramm zum Nachweis einer erfolgreichen Ablation und Staging ca. 3-4 Tage postablativ bei Patienten mit DTC. Ueberwachung des

  15. Rationality and contradictions in the competition for concessions. A (second) contribution to the Joint Practical Guide of the German Federal Cartel Office and Federal Network Agency for the allocation of electricity and gas concession tolerated and to change the concessionaire of 21 May 2015; Rationalitaet und Widersprueche im Wettbewerb um Konzessionen. Ein (zweiter) Beitrag zum Gemeinsamen Leitfaden von Bundeskartellamt und Bundesnetzagentur zur Vergabe von Strom- und Gaskonzessionsvertraegen und zum Wechsel des Konzessionsnehmers vom 21. Mai 2015

    Energy Technology Data Exchange (ETDEWEB)

    Steinbeck, David; Templin, Wolf [Boos Hummel und Wegerich Rechtsanwaelte, Berlin (Germany)

    2015-08-15

    This article follows to an essay in the ZNER of 2011 (S. 121 et seq.) for the first Joint Practical Guide of the Federal Network Agency (BNetzA) and Federal Cartel Office (FCO) from the year 2010, in which the authors had attested authorities a good ''step towards to more competition ''. In the essay is examined and shown that the Joint practical guide from the year 2010 this target could meet and what effect the now in its second edition newly revised guidelines on competition to distribution networks may have. The revision of the guidelines will be given to municipalities further action assistance to the hand, but - with few exceptions - only reproduces the jurisprudence of the staked legal framework. One proposed solution for the more diverse existing problems remains undone, whereas new problem areas are put up in several places. Nevertheless, the will of the authorities is to recognize, to avoid legal problems in the concession process and net acquisitions from the outset. [German] Dieser Beitrag knuepft an einen Aufsatz in der ZNER 2011 (S. 121 ff.) zum ersten Gemeinsamen Leitfaden von Bundesnetzagentur (BNetzA) und Bundeskartellamt (BKartA) aus dem Jahre 2010 an, in dem die Autoren den Behoerden einen richtigen ''Schritt hin zu mehr Wettbewerb'' attestiert hatten. In der Abhandlung wird untersucht und dargestellt, ob der Gemeinsame Leitfaden aus dem Jahr 2010 diesem Ziel gerecht werden konnte und welche Auswirkungen der nunmehr in der zweiten Auflage neu ueberarbeitete Leitfaden auf den Wettbewerb um Verteilnetze haben kann. Die Ueberarbeitung des Leitfadens wird den Kommunen eine weitere Handlungshilfe an die Hand gegeben, die aber - bis auf wenige Ausnahmen - den durch die Rechtsprechung abgesteckten Rechtsrahmen nur wiedergibt. Ein Loesungsvorschlag fuer die noch vielfaeltig bestehenden Probleme unterbleibt, wohingegen an mehreren Stellen neue Problemfelder aufgemacht werden. Gleichwohl ist der Wille der Behoerden

  16. Associations between the neuron-specific glucocorticoid receptor (NR3C1) Bcl-1 polymorphisms and suicide in cancer patients within the first year of diagnosis.

    Science.gov (United States)

    Park, Subin; Hong, Jin Pyo; Lee, Jong-Keuk; Park, Young-Mi; Park, Yangsoon; Jeon, Juri; Ahn, Myeong Hee; Yoon, Se Chang

    2016-07-11

    Cancer diagnosis is associated with an increased suicide risk, particularly within the first 1 year after diagnosis of cancer. Abnormal function of the hypothalamic-pituitary-adrenal axis has been implicated in the pathophysiology of depression and suicide. We examined genetic associations of the functional Bcl-1 polymorphism of (rs41423247) neuron-specific glucocorticoid receptor (NR3C1) gene, with death by suicide in cancer patients. Suicides occurring within a year of cancer diagnosis ('early suicide') were considered separately from those suicides during the second or subsequent year ('late suicide') after cancer diagnosis. The subjects consisted of 343 cancer patients admitted to a general hospital in Seoul, South Korea from 1996 to 2009, of which 182 had died by suicide and 161 were alive on December 31, 2009. Genomic DNA was extracted from formalin-fixed paraffin-embedded tissue sample of patients with cancer. We conducted a case-control association analysis of Bcl-1 polymorphism of NR3C1 gene. Subjects carrying the GG genotype of Bcl-1 polymorphism were at increased risk of early suicide when compared to those carrying the CC genotype (OR 3.80, 95 % CI 1.02-14.16, p = .047). Similarly, those individuals carrying the GG genotype (recessive mode) had an increased risk of early suicide relative to the CC or CG genotype (OR 3.71, 95 % CI 1.03-13.43, p = .045). However, there were no differences in the genotype distributions of the NR3C1 Bcl-1 polymorphism between late suicide cases and controls. Our findings suggest that the NR3C1 Bcl-1 polymorphisms may be involved in the susceptibility to suicide within the first year after cancer diagnosis among cancer patients in Korean population.

  17. Phase II trial of yttrium-90-DOTA-biotin pretargeted by NR-LU-10 antibody/streptavidin in patients with metastatic colon cancer

    International Nuclear Information System (INIS)

    Knox, S J.; Goris, M L.; Tempero, M.; Weiden, P L.; Gentner, L.; Breitz, H.; Adams, G. P.; Axworthy, D.; Gaffigan, S.; Bryan, K.; Fisher, Darrell R.; Colcher, D; Horak, I D.; Weiner, L M.

    1999-01-01

    A Phase II study of yttrium-90-tetra-azacyclododecanetetra-acetic acid-biotin (Y-90-DOTA-biotin) pretargeted by NR-LU-10 antibody/streptavidin (SA) was performed. The primary objectives of the study were to evaluate the efficacy and safety of this therapy in patients with metastatic colon cancer. Twenty-five patients were treated with a single dose of 110 mCi/m 2 (mean administered dose, 106.5-10.3 mCi/m 2 ) of Y-90-DOTA-biotin. There were three components of the therapy. Patients first received NR-LU-10/SA on day 1. A clearing agent (biotin-galactose-human serum albumin) was administered 48 h after the NR-LU-10/SA to remove residual circulating unbound NR-LU-10/SA. Lastly, 24 h after administration of clearing agent, patients received biotin-DOTA-labeled with 110 mCi/m 2 Y-90. All three components of the therapy were administered i.v. Both hematological and nonhematological toxicities were observed. Diarrhea was the most frequent grade 4 nonhematological toxicity (16%; with 16% grade 3 diarrhea). Hematological toxicity was less severe with 8% grade 3 and 8% grade 4 neutropenia and 8% grade 3 and 16% grade 4 thrombocytopenia. The overall response rate was 8%. Two partial responders had freedom from progression of 16 weeks. Four patients (16%) had stable disease with freedom from progression of 10-20 weeks. Despite the relatively disappointing results of this study in terms of therapeutic efficacy and toxicity, proof of principle was obtained for the pretargeting approach. In addition, valuable new information was obtained about normal tissue tolerance to low-dose-rate irradiation that will help to provide useful guidelines for future study designs

  18. Report on Integration of Existing Grid Models for N-R HES Interaction Focused on Balancing Authorities for Sub-hour Penalties and Opportunities

    International Nuclear Information System (INIS)

    McJunkin, Timothy; Epiney, Aaron; Rabiti, Cristian

    2017-01-01

    This report provides a summary of the effort in the Nuclear-Renewable Hybrid Energy System (N-R HES) project on the level 4 milestone to consider integration of existing grid models into the factors for optimization on shorter time intervals than the existing electric grid models with the Risk Analysis Virtual Environment (RAVEN) and Modelica [1] optimizations and economic analysis that are the focus of the project to date.

  19. Report on Integration of Existing Grid Models for N-R HES Interaction Focused on Balancing Authorities for Sub-hour Penalties and Opportunities

    Energy Technology Data Exchange (ETDEWEB)

    McJunkin, Timothy [Idaho National Lab. (INL), Idaho Falls, ID (United States); Epiney, Aaron [Idaho National Lab. (INL), Idaho Falls, ID (United States); Rabiti, Cristian [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2017-06-01

    This report provides a summary of the effort in the Nuclear-Renewable Hybrid Energy System (N-R HES) project on the level 4 milestone to consider integration of existing grid models into the factors for optimization on shorter time intervals than the existing electric grid models with the Risk Analysis Virtual Environment (RAVEN) and Modelica [1] optimizations and economic analysis that are the focus of the project to date.

  20. Rapport om kystsikringsforsøg ved anvendelse af lodrette drænrør langs Hjørring Kommunes kyststrækning

    DEFF Research Database (Denmark)

    Burcharth, Hans F.

    Med økonomisk støtte fra Erhversfremmestyrelsen har Hjørring Kommune i samarbejde med Skagen Innovationscenter, SIC, gennemført et kystsikringsforsøg med anvendelse af lodrette drænrør over en 3.7 km lang kyststraekning fra Skallerup Klit ti1 Nørlev nord. Strækningen er stationeret St. 0,0 - St 3...

  1. The Optimum Processing Parameter of Carbon Nano tubes and Thermoplastic Natural Rubber (NR/LNR/LLDPE) Composites Based on Tensile Properties

    International Nuclear Information System (INIS)

    Hazwani Halim; Syed Yusainee Syed Yahya; Sahrim Ahmad; Tarawneh, M.A.; Shamsul Bahri, A.R.

    2011-01-01

    In this study, the exact processing parameter including mixing time, rotor speed and temperature of CNTs and TPNR (NR/LNR/LLDPE) composite has been examined using tensile properties. To prepare the composite, the matrix NR/LLDPE will compatabilized using liquid natural rubber (LNR) with 40 % NR, 10 % LNR and 50 % LLDPE. Then, 2 % CNTs will be incorporated into the matrix using different processing temperature, rotor speed and mixing time. For the temperature, different temperature that used are 135, 140, 145, 150 and 155 degree and for the rotor speed 45, 50, 55, 60 and 65 rpm have been used. As for the mixing time, five different time have been investigated which is 9 min,11 min, 13 min, 15 min and 17 min. The results for Young's modulus and elongation at break show that there is the maximum increment for the composite that have been prepared using 140 degree Celsius, 55 rpm and 13 min. However when the temperature has been increased, both of these properties has been decreased. Based on these results we can conclude that the optimum processing parameter for these CNTs composite is quite similar with the composite of the matrix (TPNR) itself. (author)

  2. Clinical and biochemical function of polymorphic NR0B1 GGAA-microsatellites in Ewing sarcoma: a report from the Children's Oncology Group.

    Directory of Open Access Journals (Sweden)

    Michael J Monument

    Full Text Available The genetics involved in Ewing sarcoma susceptibility and prognosis are poorly understood. EWS/FLI and related EWS/ETS chimeras upregulate numerous gene targets via promoter-based GGAA-microsatellite response elements. These microsatellites are highly polymorphic in humans, and preliminary evidence suggests EWS/FLI-mediated gene expression is highly dependent on the number of GGAA motifs within the microsatellite.Here we sought to examine the polymorphic spectrum of a GGAA-microsatellite within the NR0B1 promoter (a critical EWS/FLI target in primary Ewing sarcoma tumors, and characterize how this polymorphism influences gene expression and clinical outcomes.A complex, bimodal pattern of EWS/FLI-mediated gene expression was observed across a wide range of GGAA motifs, with maximal expression observed in constructs containing 20-26 GGAA motifs. Relative to white European and African controls, the NR0B1 GGAA-microsatellite in tumor cells demonstrated a strong bias for haplotypes containing 21-25 GGAA motifs suggesting a relationship between microsatellite function and disease susceptibility. This selection bias was not a product of microsatellite instability in tumor samples, nor was there a correlation between NR0B1 GGAA-microsatellite polymorphisms and survival outcomes.These data suggest that GGAA-microsatellite polymorphisms observed in human populations modulate EWS/FLI-mediated gene expression and may influence disease susceptibility in Ewing sarcoma.

  3. α-Copaene is an attractant, synergistic with quercivorol, for improved detection of Euwallacea nr. fornicatus (Coleoptera: Curculionidae: Scolytinae.

    Directory of Open Access Journals (Sweden)

    Paul E Kendra

    Full Text Available The tea shot-hole borer, Euwallacea fornicatus Eichhoff, is an ambrosia beetle endemic to Asia and a pest of commercial tea, Camellia sinensis (L. Kuntze. Recently, a complex of species morphologically similar to E. fornicatus has been recognized, which includes new pests established in Israel and the USA, both in California and Florida. Collectively termed E. nr. fornicatus, these cryptic species carry symbiotic Fusarium spp. fungi, some of which cause dieback disease in susceptible hosts, which include avocado, Persea americana Miller. Due to the threat to this economically important crop, research was initiated to evaluate efficacy of kairomone-based lures for detection of the beetle in Florida (termed the Florida tea shot hole borer, FL-TSHB. A series of field tests were conducted in 2016 in commercial avocado groves known to have FL-TSHB at various population levels. All tests evaluated lures containing quercivorol (p-menth-2-en-1-ol and α-copaene, presented separately and in combination; and one test evaluated effect of trap type on beetle captures. In addition, electroantennography (EAG was used to quantify female olfactory responses to lure emissions. This study identified (--α-copaene as a new attractant for FL-TSHB, equivalent in efficacy to quercivorol (the standard lure for Euwallacea detection in the USA; however, the combination of lures captured significantly more FL-TSHB than either lure alone. This combination resulted in synergistic attraction at two field sites and additive attraction at a third site. Sticky panel traps captured more FL-TSHB than comparably-baited Lindgren funnel traps. Females engaged in host-seeking flight from 11:00 to 16:00 hr (EST, with peak numbers observed between 12:00 and 13:00 hr. EAG analyses confirmed olfactory chemoreception of both kairomones, with a higher response elicited with the combination of volatiles. Results indicate that detection of pest E. nr. fornicatus in Florida can be improved

  4. Influence of NR3C1 and VDR polymorphisms on stable warfarin dose in patients with mechanical cardiac valves.

    Science.gov (United States)

    Lee, Kyung Eun; Chung, Jee Eun; Yi, Boram; Cho, Yoon Jeong; Kim, Hyun Jeong; Lee, Gwan Yung; Kim, Joo Hee; Chang, Byung Chul; Gwak, Hye Sun

    2017-06-01

    The aim of this study was to evaluate the associations between polymorphisms of VKORC1, CYP2C9, CYP4F2, NR3C1 and VDR genes and stable warfarin doses in Korean patients with mechanical heart valves. Seventeen single-nucleotide polymorphisms (SNPs) in 204 patients with stable warfarin dose were analyzed: VKORC1 (rs9934438), CYP2C9 (rs1057910), CYP4F2 (rs2108622), NR3C1 (rs41423247, rs1800445, rs56149945, rs10052957, rs6198, rs33388, rs6196, and rs244465), and VDR (rs1544410, rs11568820, rs731236, rs757343, rs7975232, and rs2228570). Statistical analyses were conducted to evaluate the associations of gene variations with stable warfarin dose. Number needed to genotype was obtained by calculating the percentage of patients whose predicted dose was at least 20% higher or lower than the actual stable dose. The combined genotypes of rs7975232 and rs2228570 of the VDR gene revealed a significant association with stable warfarin dose, along with VKORC1, CYP2C9, and CYP4F2 polymorphisms. Patients with the genotype combination GT,TT/CT,CC of VDR rs7975232/rs2228570 required significantly higher stable warfarin dose (5.79±2.02mg) than those with the other genotypic combinations (5.19±1.78mg, p=0.034). Multivariate analysis showed that VDR rs7975232/rs2228570 explained 2.0% of the 47.5% variability in overall warfarin dose. Adding VDR SNP combinations to the base model including non-genetic variables (age, sex, and body weight) and genetic variables (VKORC1 rs9934438, CYP2C9 rs1057910, and CYP4F2 rs2108622) gave a number needed to genotype of 41. This study showed that stable warfarin dose is associated with VDR SNPs along with VKORC1, CYP2C9, and CYP4F2 SNPs. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Effect of sigma phase in the repassivation potential of austenitic-ferritic stainless steel SEW 410 Nr. 14517; Efeito da fase sigma no potencial de repassivacao do aco inoxidavel austeno-ferritico SEW 410 Nr. 14517

    Energy Technology Data Exchange (ETDEWEB)

    Itman Filho, A.; Pimenta, C.C.; Santos, C.M.L. [Instituto Federal do Espirito Santo, Vitoria (Brazil)], e-mail: andrei@ifes.edu.br; Casteletti, L.C. [Escola de Engenharia de Sao Carlos - EESC/USP, SP (Brazil)

    2010-07-01

    The austenitic-ferritic stainless steels, due to the optimum compromise between mechanical properties and corrosion resistance, are being used in many applications, as chemical and oil industries. In the oil exploitation, the effect of erosion-corrosion in acid environment is responsible for the increment in the equipment maintenance costs. Regarding the interest in researches of deep water oil exploitation, the proposal of this study was to evaluate the effect of the heat treatment at 850 degree C of the austenitic-ferritic stainless steel SEW 410 Nr.14517. Microstructural characterizations, microhardness measurements of the phases and corrosion tests were accomplished to evaluate the samples. The pitting potential values were obtained by galvanostatic tests in H{sub 2}SO{sub 4} acid solution. The results showed that the volumetric percentages of sigma phase increased while the ferritic phase percentages decreased, when the heat treatment time increased. The sigma phase promoted hardness increase with little corrosion resistance decrease in the austenitic-ferritic stainless steel, too. (author)

  6. Research on the subject of air pollution and forest damage in the period from 1984 to 1995. Report on results; Forschung zum Schwerpunkt Luftverunreinigungen und Waldschaeden 1984-1995. Ergebnisbericht

    Energy Technology Data Exchange (ETDEWEB)

    Reuther, M.; Kirchner, M.; Roesel, K.; Weissgerber, K.

    1996-02-01

    , Luftchemie, Stoffeintrag ueber Physiologie und Biochemie der Baeume bis hin zu bodenkundlichen Untersuchungen einschliesslich Feinwurzeln und Mykorrhiza. Die Freilanduntersuchungen wurden an den interdisziplinaer bearbeiteten Forschungsstandorten der bayerischen Waldschadensforschung durchgefuehrt. Einen besonderen Arbeitsschwerpunkt stellte der alpine Forschungsstandort Wank dar. Laborarbeiten sowie Experimente unter kontrollierten Bedingungen der GSF-Expositionskammern traten ergaenzend hinzu. Auch wenn viele Fragen zum Verstaendnis der stofflichen Vorgaenge und Veraenderungen im Oekosystem Wald noch offen sind, lassen sich aus den Ergebnissen der Waldschadensforschung in einigen Teilbereichen weitgehend abschliessliche Aussagen treffen. Die Ergebnisse werden zusammenfassend dargestellt. (orig.)

  7. German S3 guideline for renal cell carcinoma. Presentation and discussion of essential aspects for the radiation oncologist; Die deutsche S3-Leitlinie zum Nierenzellkarzinom. Darstellung und Diskussion wesentlicher Aspekte fuer den Radioonkologen

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, Arndt-Christian [Eberhard Karls University, Department of Radiation Oncology, Tuebingen (Germany); Oorschot, Birgitt van [Julius Maximilians University, Department of Radiation Oncology, Wuerzburg (Germany); Micke, Oliver [Franziskus Hospital Bielefeld, Department of Radiotherapy and Radiation Oncology, Bielefeld (Germany); Guckenberger, Matthias [University of Zurich, Department of Radiation Oncology, Zuerich (Switzerland)

    2018-01-15

    We aim to introduce and discuss the statements and recommendations of the German S3 guideline on renal cell cancer for daily practice of radiation oncologists. This report comprises indication, treatment decision, dose prescription and current literature including treatment of oligometastatic disease. According to different stages of the disease and the structure of the guideline we focus on five treatment situations and recommendations for decision making: (1) Neo-/adjuvant treatment before or after nephrectomy: No indication for radiotherapy. (2) Small renal mass: Stereotactic ablative radiotherapy is currently seen as experimental option due to small patient numbers reported in the literature. However, local tumor control achieved by SBRT appears favourable with >90% at 2 years. (3) Oligometastasis: Radiation treatment with higher local doses or stereotactic treatment is possible after interdisciplinary discussion. Indications for palliative (4) and symptomatic treatment (5) are not different compared to other tumor entities. Currently, there is no evidence-based indication for radiation treatment in the primary setting (adjuvant/neoadjuvant or definitive) of renal cell cancer. In the future stereotactic radiotherapy should have a stronger role in the treatment of medically inoperable patients with primary renal cell cancer and especially in the setting of oligometastasis. (orig.) [German] Ziel dieser Arbeit ist es, dem Radioonkologen die Empfehlungen und Diskussionswege der neuen S3-Leitlinie zum Nierenzellkarzinom fuer die taegliche Praxis pointiert darzustellen. Es werden Indikationsstellung, Dosisverschreibung, technische Durchfuehrung sowie aktuelle Literatur fuer den Strahlentherapeuten, vor allem mit Fokus auf die Behandlung der Oligometastasierung, zusammengefasst. Entsprechend der unterschiedlichen Erkrankungsstadien und der Gliederung der Leitlinie werden fuenf radiotherapeutische Therapiesituationen und die Empfehlungen dazu vorgestellt: (1

  8. Habitus in the Classroom: the Relevance of Student Heterogeneity and Departmental Culture for Learner-Oriented Didactics in Teaching Sociology/Didaktische Strategien zum Umgang mit habitueller Vielfalt und spezifischer Fachkultur in Soziologie

    Directory of Open Access Journals (Sweden)

    Klarissa Lueg

    2011-06-01

    Full Text Available "The relevance of habitus, social origin and the mechanisms of exclusion as applied by the university system has often been discussed in current research. It has been stated that opportunities of students are impaired by field-of-study orientations and drop-out rates. In contrast to this, the aspect how university teachers can practically deal with this knowledge is less elaborated. In view of this, this article presents methods of how learner-oriented approaches, with special reference to the heterogeneity of student milieus, can be implemented in teaching sociology. On the basis of reviewing theoretical approaches and recent empirical data, this article points out a what data are relevant for the operationalisation of learner oriented didactics,b what concrete problems might occur in the teacher-student-relationship and c what techniques are to be applied by sociology teachers in handling classroom problems. The results demonstrate that teaching methods which adequately respond to heterogeneity within the culture of sociology departments are imperative and available. Still, to establish equal opportunities, a more practical turn in a hitherto predominantly theoretical discussion is clearly needed.Chancenungleichheit durch Passungsdifferenzen unterschiedlicher sozialer Milieus zum Hochschulsystem ist schon häufig theoretisch diskutiert worden. Zudem geben empirische Untersuchungen detaillierte Einblicke in studentische Milieus und Fachkulturen. Bei der Literaturrezeption entsteht der Eindruck, eine didaktische Reaktion auf das Problem der hochschulinternen Exklusionsmechanismen könnte nicht dringend genug sein. Die Konzeption entsprechender didaktischen Ansätze wird aber wenig thematisiert. Dieser Artikel hat den Anspruch, konkrete Umsetzungsmöglichkeiten von lernerzentrierten didaktischen Ansätzen im Fach Soziologie aufzuzeigen. In einem Fach, das grundsätzlich sensibel für Ungleichheiten ist, fehlen trotzdem heterogenit

  9. Determination and calculation of hazardous incident scenarios in accordance with the 3rd hazardous incident administrative instruction. Vol. 1; Ermittlung und Berechnung von Stoerfallablaufszenarien nach Massgabe der 3. Stoerfallverwaltungsvorschrift. Bd. 1: Methodischer Teil zum Erarbeiten von Stoerfallablaufszenarien

    Energy Technology Data Exchange (ETDEWEB)

    Kaiser, W.; Rogazewski, P.; Schindler, M. [TUeV Anlagentechnik GmbH, Berlin-Schoeneberg (Germany). Unternehmensgruppe TUeV Rheinland/Berlin-Brandenburg; Acikalin, A.; Albrecht, M.; Lambert, M.; Steinbach, J. [Technische Univ. Berlin (Germany)

    2000-07-01

    Stoerfaellen sowie zu Bedingungen des Standortes und der Anlage die erforderlichen Grundannahmen zum Stoerfallablauf gewinnen kann. Unterschieden nach Stofffreisetzungen, Explosionen und Braenden wird jeweils auf die konkreteren Hinweise im Anhang 1 zur Abschaetzung von Quelltermen, im Anhang 2 zur Abschaetzung der Ausbreitung und im Anhang 3 zur Abschaetzung der Auswirkungen verwiesen. Der Anhang 4 enthaelt einige Erlaeuterungen zu den Anhaengen 1 bis 3. Mit einer Sammlung von 15 Beispielen fuer Stoerfallablaufszenarien wird im Anhang 5 das im Leitfaden beschriebene methodische Vorgehen veranschaulicht und erlaeutert. Im Anhang 6 werden die auf dem deutschen Markt erhaeltlichen DV-Programme (Software) zur Berechnung von Stoerfallablaufszenarien beschrieben und kommentiert. Der Band 2 untermauert das Vorgehen im Hinblick auf Berechnungsmethoden, aktuelle Modelle und Modellgleichungen aus wissenschaftlicher Sicht. (orig.)

  10. Fabrication of TiO_2 nanorod assembly grafted rGO (rGO@TiO_2-NR) hybridized flake-like photocatalyst

    International Nuclear Information System (INIS)

    Lv, Kangle; Fang, Shun; Si, Lingling; Xia, Yang; Ho, Wingkei; Li, Mei

    2017-01-01

    Highlights: • TiO_2 nanorod assembly grafted with GO hybrid was successfully fabricated. • TiO_2 nanorods can reduce the aggregation of TiO_2 nanoparticles on graphene. • This unique structure facilitates the injection of electron from TiO_2 to graphene. - Abstract: To efficiently separate the photo-generated electron–hole pairs of TiO_2 hybrid, anatase TiO_2 nanorod assembly grafted reduced graphene oxides (rGO@TiO_2-NR) hybrid was successfully fabricated using potassium titanium oxalate (PTO) and graphene oxides (GO) as starting materials and diethylene glycol (DEG) as reductant. The effect of GO content on the structure and photocatalytic activity of rGO@TiO_2-NR composite was systematically studied. Results show that, in the absence of GO, only TiO_2 microsphere assembly is obtained from TiO_2 nanorods. The presence of GO results in the formation of a flake-like TiO_2-nanorod-assembled grafted rGO hybrid. The photocatalytic activity of rGO@TiO_2-NR composite increases first and then decreases with increase in the amount of GO from 0 wt.% to 10 wt.%. The hybridized S4 sample prepared with 4 wt.% GO possesses the highest photocatalytic activity with a constant rate of 0.039 min"−"1 in the photocataytic degradation of Brilliant X-3B dye (X3B); this sample was enhanced more than three times when compared with pure TiO_2 sample (0.012 min"−"1). The enhanced photocatalytic activity of the rGO@TiO_2-NR hybrid was attributed to the strong interaction between TiO_2 nanorods and rGO. The unique hierarchical structure of 1D nanorod assembly TiO_2–rGO flakes facilitates the injection and transfer of photo-generated electrons from TiO_2 to graphene, thus retarding the recombination of electron–hole pairs and enhancing the photocatalytic activity. The enlarged BET surface areas, not only increasing the number of active sites, but also facilitating the adsorption of the dye, and improved light-harvesting ability also contribute to the enhanced photoreactivity

  11. Fabrication of TiO2 nanorod assembly grafted rGO (rGO@TiO2-NR) hybridized flake-like photocatalyst

    Science.gov (United States)

    Lv, Kangle; Fang, Shun; Si, Lingling; Xia, Yang; Ho, Wingkei; Li, Mei

    2017-01-01

    To efficiently separate the photo-generated electron-hole pairs of TiO2 hybrid, anatase TiO2 nanorod assembly grafted reduced graphene oxides (rGO@TiO2-NR) hybrid was successfully fabricated using potassium titanium oxalate (PTO) and graphene oxides (GO) as starting materials and diethylene glycol (DEG) as reductant. The effect of GO content on the structure and photocatalytic activity of rGO@TiO2-NR composite was systematically studied. Results show that, in the absence of GO, only TiO2 microsphere assembly is obtained from TiO2 nanorods. The presence of GO results in the formation of a flake-like TiO2-nanorod-assembled grafted rGO hybrid. The photocatalytic activity of rGO@TiO2-NR composite increases first and then decreases with increase in the amount of GO from 0 wt.% to 10 wt.%. The hybridized S4 sample prepared with 4 wt.% GO possesses the highest photocatalytic activity with a constant rate of 0.039 min-1 in the photocataytic degradation of Brilliant X-3B dye (X3B); this sample was enhanced more than three times when compared with pure TiO2 sample (0.012 min-1). The enhanced photocatalytic activity of the rGO@TiO2-NR hybrid was attributed to the strong interaction between TiO2 nanorods and rGO. The unique hierarchical structure of 1D nanorod assembly TiO2-rGO flakes facilitates the injection and transfer of photo-generated electrons from TiO2 to graphene, thus retarding the recombination of electron-hole pairs and enhancing the photocatalytic activity. The enlarged BET surface areas, not only increasing the number of active sites, but also facilitating the adsorption of the dye, and improved light-harvesting ability also contribute to the enhanced photoreactivity of rGO@TiO2-NR hybrid.

  12. Fabrication of TiO{sub 2} nanorod assembly grafted rGO (rGO@TiO{sub 2}-NR) hybridized flake-like photocatalyst

    Energy Technology Data Exchange (ETDEWEB)

    Lv, Kangle, E-mail: lvkangle@mail.scuec.edu.cn [Department of Science and Environmental Studies, The Hong Kong Institute of Education, Taipo, N.T., Hong Kong (China); Key Laboratory of Catalysis and Materials Science of the State Ethnic Affairs Commission and Ministry of Education, College of Resources and Environmental Science, South-Central University for Nationalities, Wuhan 430074 (China); Fang, Shun; Si, Lingling; Xia, Yang [Key Laboratory of Catalysis and Materials Science of the State Ethnic Affairs Commission and Ministry of Education, College of Resources and Environmental Science, South-Central University for Nationalities, Wuhan 430074 (China); Ho, Wingkei, E-mail: keithho@ied.edu.hk [Department of Science and Environmental Studies, The Hong Kong Institute of Education, Taipo, N.T., Hong Kong (China); Li, Mei [Key Laboratory of Catalysis and Materials Science of the State Ethnic Affairs Commission and Ministry of Education, College of Resources and Environmental Science, South-Central University for Nationalities, Wuhan 430074 (China)

    2017-01-01

    Highlights: • TiO{sub 2} nanorod assembly grafted with GO hybrid was successfully fabricated. • TiO{sub 2} nanorods can reduce the aggregation of TiO{sub 2} nanoparticles on graphene. • This unique structure facilitates the injection of electron from TiO{sub 2} to graphene. - Abstract: To efficiently separate the photo-generated electron–hole pairs of TiO{sub 2} hybrid, anatase TiO{sub 2} nanorod assembly grafted reduced graphene oxides (rGO@TiO{sub 2}-NR) hybrid was successfully fabricated using potassium titanium oxalate (PTO) and graphene oxides (GO) as starting materials and diethylene glycol (DEG) as reductant. The effect of GO content on the structure and photocatalytic activity of rGO@TiO{sub 2}-NR composite was systematically studied. Results show that, in the absence of GO, only TiO{sub 2} microsphere assembly is obtained from TiO{sub 2} nanorods. The presence of GO results in the formation of a flake-like TiO{sub 2}-nanorod-assembled grafted rGO hybrid. The photocatalytic activity of rGO@TiO{sub 2}-NR composite increases first and then decreases with increase in the amount of GO from 0 wt.% to 10 wt.%. The hybridized S4 sample prepared with 4 wt.% GO possesses the highest photocatalytic activity with a constant rate of 0.039 min{sup −1} in the photocataytic degradation of Brilliant X-3B dye (X3B); this sample was enhanced more than three times when compared with pure TiO{sub 2} sample (0.012 min{sup −1}). The enhanced photocatalytic activity of the rGO@TiO{sub 2}-NR hybrid was attributed to the strong interaction between TiO{sub 2} nanorods and rGO. The unique hierarchical structure of 1D nanorod assembly TiO{sub 2}–rGO flakes facilitates the injection and transfer of photo-generated electrons from TiO{sub 2} to graphene, thus retarding the recombination of electron–hole pairs and enhancing the photocatalytic activity. The enlarged BET surface areas, not only increasing the number of active sites, but also facilitating the adsorption of

  13. The LIM domain protein FHL2 interacts with the NR5A family of nuclear receptors and CREB to activate the inhibin-α subunit gene in ovarian granulosa cells.

    Science.gov (United States)

    Matulis, Christina K; Mayo, Kelly E

    2012-08-01

    Nuclear receptor transcriptional activity is enhanced by interaction with coactivators. The highly related nuclear receptor 5A (NR5A) subfamily members liver receptor homolog 1 and steroidogenic factor 1 bind to and activate several of the same genes, many of which are important for reproductive function. To better understand transcriptional activation by these nuclear receptors, we sought to identify interacting proteins that might function as coactivators. The LIM domain protein four and a half LIM domain 2 (FHL2) was identified as interacting with the NR5A receptors in a yeast two-hybrid screen of a human ovary cDNA library. FHL2, and the closely related FHL1, are both expressed in the rodent ovary and in granulosa cells. Small interfering RNA-mediated knockdown of FHL1 and FHL2 in primary mouse granulosa cells reduced expression of the NR5A target genes encoding inhibin-α and P450scc. In vitro assays confirmed the interaction between the FHL and NR5A proteins and revealed that a single LIM domain of FHL2 is sufficient for this interaction, whereas determinants in both the ligand binding domain and DNA binding domain of NR5A proteins are important. FHL2 enhances the ability of both liver receptor homolog 1 and steroidogenic factor 1 to activate the inhibin-α subunit gene promoter in granulosa cells and thus functions as a transcriptional coactivator. FHL2 also interacts with cAMP response element-binding protein and substantially augments activation of inhibin gene expression by the combination of NR5A receptors and forskolin, suggesting that FHL2 may facilitate integration of these two signals. Collectively these results identify FHL2 as a novel coactivator of NR5A nuclear receptors in ovarian granulosa cells and suggest its involvement in regulating target genes important for mammalian reproduction.

  14. Parameters for Novel Production of Fruity Floral Fragrance Ester (Geranyl Butyrate) by Locally Isolated Lipase Geobacillus thermodenitrificans nr68 (LGT)

    Science.gov (United States)

    Nik Raikhan, N. H.

    2018-05-01

    Geranyl butyrate has been synthesized successfully using our locally isolated lipase Geobacillus thermodenitrificans nr68 (LGT) as the fragrance ester with aim to be used in a nanotechnology fragrance application. We have used and modified few parameters from the previous research and then, continued with optimization of the synthesis by looking into degree of esterification and water content in the system. Butyric acid (C4), stearic acid (C18: 0), caprylic acid (C8), linolenic acid (C18: 3), myristic acid (C14), linoleic acid (C18: 2) and oleic acid (C18: 1) were used in the substrate selection. The yield of geranyl butyrate before the optimization was 31.68±0.01%. The optimum parameters for the synthesis of geranyl butyrate were recorded as temperature of 65°C, shaking rate at 200 rpm, 5.0 ml of geraniol and 0.40 ml of butyric acid and 4.0 ml of n-butanol and 0.40 ml of oleic acid. After the optimization, geranyl butyrate synthesis was increased by 297% as to compare with the value before the parameters were optimized. We also have significantly reduced water content as a byproduct of the esterification and managed to run the system a success. The ability thermotolerant lipase from Geobacillus thermodenitrificans (LGT) in this synthesis is novel to Malaysian fragrance industry.

  15. Genetic variation and phylogenetic relationship analysis of Jatropha curcas L. inferred from nrDNA ITS sequences.

    Science.gov (United States)

    Guo, Guo-Ye; Chen, Fang; Shi, Xiao-Dong; Tian, Yin-Shuai; Yu, Mao-Qun; Han, Xue-Qin; Yuan, Li-Chun; Zhang, Ying

    2016-01-01

    Genetic variation and phylogenetic relationships among 102 Jatropha curcas accessions from Asia, Africa, and the Americas were assessed using the internal transcribed spacer region of nuclear ribosomal DNA (nrDNA ITS). The average G+C content (65.04%) was considerably higher than the A+T (34.96%) content. The estimated genetic diversity revealed moderate genetic variation. The pairwise genetic divergences (GD) between haplotypes were evaluated and ranged from 0.000 to 0.017, suggesting a higher level of genetic differentiation in Mexican accessions than those of other regions. Phylogenetic relationships and intraspecific divergence were inferred by Bayesian inference (BI), maximum parsimony (MP), and median joining (MJ) network analysis and were generally resolved. The J. curcas accessions were consistently divided into three lineages, groups A, B, and C, which demonstrated distant geographical isolation and genetic divergence between American accessions and those from other regions. The MJ network analysis confirmed that Central America was the possible center of origin. The putative migration route suggested that J. curcas was distributed from Mexico or Brazil, via Cape Verde and then split into two routes. One route was dispersed to Spain, then migrated to China, eventually spreading to southeastern Asia, while the other route was dispersed to Africa, via Madagascar and migrated to China, later spreading to southeastern Asia. Copyright © 2016 Académie des sciences. Published by Elsevier SAS. All rights reserved.

  16. Environmental contaminants activate human and polar bear (Ursus maritimus) pregnane X receptors (PXR, NR1I2) differently.

    Science.gov (United States)

    Lille-Langøy, Roger; Goldstone, Jared V; Rusten, Marte; Milnes, Matthew R; Male, Rune; Stegeman, John J; Blumberg, Bruce; Goksøyr, Anders

    2015-04-01

    Many persistent organic pollutants (POPs) accumulate readily in polar bears because of their position as apex predators in Arctic food webs. The pregnane X receptor (PXR, formally NR1I2, here proposed to be named promiscuous xenobiotic receptor) is a xenobiotic sensor that is directly involved in metabolizing pathways of a wide range of environmental contaminants. In the present study, we comparably assess the ability of 51 selected pharmaceuticals, pesticides and emerging contaminants to activate PXRs from polar bears and humans using an in vitro luciferase reporter gene assay. We found that polar bear PXR is activated by a wide range of our test compounds (68%) but has a slightly more narrow ligand specificity than human PXR that was activated by 86% of the 51 test compounds. The majority of the agonists identified (70%) produces a stronger induction of the reporter gene via human PXR than via polar bear PXR, however with some notable and environmentally relevant exceptions. Due to the observed differences in activation of polar bear and human PXRs, exposure of each species to environmental agents is likely to induce biotransformation differently in the two species. Bioinformatics analyses and structural modeling studies suggest that amino acids that are not part of the ligand-binding domain and do not interact with the ligand can modulate receptor activation. Copyright © 2015. Published by Elsevier Inc.

  17. OBSERVAŢII REFERITOARE LA AMENDAMENTELE OPERATE ÎN CODUL PENAL PRIN LEGEA NR.60/2016

    Directory of Open Access Journals (Sweden)

    Vitalie STATI

    2016-11-01

    Full Text Available Obiectul prezentului studiu îl constituie modificările şi completările operate în Codul penal prin Legea nr.60/2016. Acestea vizează: persoana juridică în calitate de subiect al infracţiunii; confiscarea specială; spălarea banilor; actul terorist; finanţarea terorismului; abuzul de putere sau abuzul de serviciu. În procesul analizei, sunt examinate proiectul care se află la baza Legii nr.60/2016, Nota informativă la acest proiect şi alte asemenea acte preparatorii. De asemenea, se face referire la Convenţia privind spălarea, descoperirea, sechestrarea şi confiscarea produselor infracţiunii şi finan­ţarea terorismului, adoptată la Varşovia la 16.05.2005, precum şi la raportul explicativ la această Convenţie. Scopul prezentului studiu constă în stabilirea efectelor pozitive şi a celor negative ale adoptării modificărilor şi completărilor operate în Codul penal prin Legea nr.60/2016. În rezultatul investigaţiei efectuate se ajunge la concluzia că nu toate amendamentele operate prin legea amintită au tangenţă cu domeniile ce ţin de prevenirea şi combaterea spălării banilor şi a finanţării terorismului. Nu toate aceste amendamente pot asigura armonizarea legislaţiei naţionale penale cu stan­dardele stabilite de Curtea Europeană pentru Drepturile Omului. Unele din aceste amendamente sunt îndoielnice sub aspectul calităţii lor tehnico-legislative şi/sau în ce priveşte aportul lor în planul eficientizării apărării ordinii de drept. Impresia de ansamblu este că nu preocuparea pentru interesul social a constituit scopul principal al adoptării Legii nr.60/2016.SOME REMARKS ON THE AMENDMENTS OPERATED TO THE PENAL CODE BY LAW No.60/2016The object of this study is formed by the modifications and additions operated to the Penal Code by Law No.60/2016. The content of the respective initiatives addresses the following elements: the legal person as the subject of the offence; special confiscation

  18. Description of work vadose drilling at the 1301-N and 1325-N facilities, 100-NR-1 operable unit

    International Nuclear Information System (INIS)

    1994-08-01

    This description of work (DOW) details the field activities associated with the sampling of the vadose zone soils beneath the 1301-N and 1325-N cribs and trenches and will serve as a field guide for those performing the work. These activities are undertaken pursuant to the Hanford Federal Facility Agreement and Consent Order (Ecology et al., 1994a) Milestone M-16-94-01H-Tl and the June 30, 1994 Milestone Change Request M-16-94-02 (Ecology et al., 1994b). Three vadose zone borings, 1301-N-1, 1301-N-2, and 1325-N-1, will be constructed to investigate the vertical and horizontal distribution of radionuclide contamination in sediments beneath the cribs and trenches. The boreholes are also intended to intersect subsurface areas that may have been contaminated by dangerous wastes, i.e., metals, in effluent disposed during past operation of the facilities. This limited field investigation will provide data for the evaluation of remedial alternatives. Data from the investigation are expected to confirm that the cribs and trenches are high priority sites in the 100-NR-1 operable unit. Data, from the investigation will be used to evaluate alternatives for closure of the 1301-N and 1325-N sites. The contaminants of potential concern (COPCs) for the 1301-N/1325-N limited field investigation are presented in Table 1

  19. Effect of Rice Husk Surface Modification by LENR the on Mechanical Properties of NR/ HDPE Reinforced Rice Husk Composite

    International Nuclear Information System (INIS)

    Rahmadini Syafri; Ishak Ahmad; Ibrahim Abdullah

    2011-01-01

    Surface modification of rice husk (RH) with alkali pre-treatment (NaOH solution 5 % w/ v) was carried out at the initial state to investigate the effect of surface treatment of fibre on the surface interaction between fibre and rubber. Further modification of RH surfaces after alkali treatment was using Liquid Epoxidized Natural Rubber (LENR) coating at three concentrations, 5 %, 10 %, and 20 % wt LENR solution in toluene. Interfacial morphology and chemical reactions between RH fibre and rubber were analyzed by FTIR and Scanning Electron Microscope (SEM). It was found that 10 % wt LENR solution gave the optimum interaction between fibre and rubber. Matrix and composite blends derived from 60 % natural rubber (NR), 40 % high density polyethylene (HDPE) reinforced with RH fibre were prepared using an internal mixer (Brabender Plasticoder). Result showed that pre-treatment of RH treated with 5 % NaOH followed by treatment with 10 % LENR solution given the maximum interaction between fibre and matrix that gave rise to better mechanical properties of the composites. (author)

  20. Mechanical and Morphological Properties of Waste Short Nylon Fibers and Nanoclay Reinforced NR/SBR Rubber Nanocomposites

    Directory of Open Access Journals (Sweden)

    Mohammad Andideh

    2013-02-01

    Full Text Available Natural rubber and styrene butadiene rubber (NR/SBR reinforced with short nylon fibers along with nanoclay (Cloisite 15A hybrid composites were prepared in an internal and a two roll-mill mixer by a three-step mixingprocess. The effects of fiber content at a constant loading of 3 wt% nanoclay were studied on the microstructure, mechanical and morphological properties of the prepared nanocomposites. The adhesion between the fiber and the rubber was enhanced by the addition of a dry bonding system consisting of resorcinol, hexamethylene tetramine and hydrated silica (HRH. The curing characteristics of the composites were determined and subsequently vulcanized at 150°C using a hot press. It was observed that the cure time and swelling index of the composites decreased while maximum torque, and cure rate increased with increasing of short fiber and nanoclay contents. Thestructure and fracture surface morphology of the nanocomposites were characterized using X-ray diffraction, scanning electron microscopy. X-ray diffraction results of nanocomposites indicated that the interlayer distance of silicate layers increased. The mechanical properties (tensile, tear strength, elongation-at-break and hardness ofnanocomposites containing virgin and waste fibers in the longitudinal direction are compared.

  1. Das Unsichtbare sichtbar machen. Zum vergessenen Beitrag der alten deutschen Frauenbewegung zur Wohn- und Siedlungsreform im frühen 20. Jahrhundert Making the Invisible Visible—On a Forgotten Contribution to the Early German Women’s Movement: Housing Reform at the Beginning of the Twentieth Century

    Directory of Open Access Journals (Sweden)

    Claudia Wucherpfennig

    2007-03-01

    Full Text Available Der Wohnungs- und Städtebau der Moderne gilt weitgehend als eine „Männerdomäne”. In ihrer Schrift Die Wohnungsfrage ist Frauensache! zeigen Ulla Terlinden und Susanna von Oertzen demgegenüber die vielfältigen und einflussreichen Beiträge der alten deutschen Frauenbewegung zur Wohnungsreform des frühen 20. Jahrhunderts und zum Neuen Bauen auf.The building of apartments and cities in modernity is seen often as a “male domain.” In the text The Apartment Question is Women’s Work! (Die Wohungsfrage ist Frauensache!, Ulla Terlinden and Susanna von Oertzen present the multiple and influential contributions of the early German women’s movement to housing reform at the beginning of the twentieth century as well as to Neues Bauen, an architectural style of the 1920s.

  2. Management of auxiliary aggregates - development and testing of methods for improved use of auxiliary aggregates of diesel and spark ignition engines in stationary and unstationary operation. Final report; Nebenaggregate-Management - Entwicklung und Erprobung von Verfahren zum verbesserten Einsatz der Nebenaggregate an Diesel- und Ottomotoren bei stationaerem und instationaerem Betrieb. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, M; Lenzen, B; Groef, S; Isermann, R [Technische Univ. Darmstadt (Germany). Inst. fuer Regelungstechnik; Hohenberg, G [Technische Univ. Darmstadt (Germany). Fachgebiet Verbrennungskraftmaschinen

    1998-07-01

    Aggregate management is aimed at aggregate operation in consideration of demand, consumption and exhaust. Demands on reliability are high. Investigations were made using modelling, simulation, model reduction and identification, with the focus on model reduction. The resulting concepts for consumption reduction vary depending on the vehicle type and application. [German] Das Nebenaggregate-Management hat den bedarfs-, verbrauchs- und abgasorientierten Betrieb der Nebenaggregate im Kraftfahrzeug zum Ziel. Der sehr weit gespannte Einsatzbereich von Kraftfahrzeugen und die hohen Anforderungen an die Betriebszuverlaessigkeit stellen entsprechende Anforderungen an das Management. Zur Untersuchung eines verbesserten Betriebs von Nebenaggregaten wrden Verfahren der Regelungstechnik wie z.B. Modellbildung, Simulation, Modellreduktion und Identifikation eingesetzt, wobei der Modellreduktion in anbetracht des komplexen Gesamtsystems Kraftfahrzeug eine besondere Bedeutung zukommt. Ergebnisse der Untersuchungen sind Konzepte zur Verringerung des Verbrauchsanteils der Nebenaggregate, die je nach Fahrzeugtyp und Einsatzzweck variieren. (orig.)

  3. Environmental contaminants activate human and polar bear (Ursus maritimus) pregnane X receptors (PXR, NR1I2) differently

    Energy Technology Data Exchange (ETDEWEB)

    Lille-Langøy, Roger, E-mail: Roger.lille-langoy@bio.uib.no [University of Bergen, Department of Biology, P.O. Box 7803, N-5020 Bergen (Norway); Goldstone, Jared V. [Woods Hole Oceanographic Institution, 266 Woods Hole Road, 02543-1050 Woods Hole, MA (United States); Rusten, Marte [University of Bergen, Department of Molecular Biology, P.O. Box 7803, N-5020 Bergen (Norway); Milnes, Matthew R. [Mars Hill University, 100 Athletic Street, Box 6671, Mars Hill, 28754 NC (United States); Male, Rune [University of Bergen, Department of Molecular Biology, P.O. Box 7803, N-5020 Bergen (Norway); Stegeman, John J. [Woods Hole Oceanographic Institution, 266 Woods Hole Road, 02543-1050 Woods Hole, MA (United States); Blumberg, Bruce [University of California, Irvine, 92697 CA (United States); Goksøyr, Anders [University of Bergen, Department of Biology, P.O. Box 7803, N-5020 Bergen (Norway)

    2015-04-01

    Background: Many persistent organic pollutants (POPs) accumulate readily in polar bears because of their position as apex predators in Arctic food webs. The pregnane X receptor (PXR, formally NR1I2, here proposed to be named promiscuous xenobiotic receptor) is a xenobiotic sensor that is directly involved in metabolizing pathways of a wide range of environmental contaminants. Objectives: In the present study, we comparably assess the ability of 51 selected pharmaceuticals, pesticides and emerging contaminants to activate PXRs from polar bears and humans using an in vitro luciferase reporter gene assay. Results: We found that polar bear PXR is activated by a wide range of our test compounds (68%) but has a slightly more narrow ligand specificity than human PXR that was activated by 86% of the 51 test compounds. The majority of the agonists identified (70%) produces a stronger induction of the reporter gene via human PXR than via polar bear PXR, however with some notable and environmentally relevant exceptions. Conclusions: Due to the observed differences in activation of polar bear and human PXRs, exposure of each species to environmental agents is likely to induce biotransformation differently in the two species. Bioinformatics analyses and structural modeling studies suggest that amino acids that are not part of the ligand-binding domain and do not interact with the ligand can modulate receptor activation. - Highlights: • Comparative study of ligand activation of human and polar bear PXRs. • Polar bear PXR is a promiscuous ligand-activated nuclear receptor but less so than human PXR. • Environmental contaminants activate human and polar bear PXRs differently. • Expression and ligand promiscuity indicate that PXR is a xenosensor in polar bears.

  4. Environmental contaminants activate human and polar bear (Ursus maritimus) pregnane X receptors (PXR, NR1I2) differently

    International Nuclear Information System (INIS)

    Lille-Langøy, Roger; Goldstone, Jared V.; Rusten, Marte; Milnes, Matthew R.; Male, Rune; Stegeman, John J.; Blumberg, Bruce; Goksøyr, Anders

    2015-01-01

    Background: Many persistent organic pollutants (POPs) accumulate readily in polar bears because of their position as apex predators in Arctic food webs. The pregnane X receptor (PXR, formally NR1I2, here proposed to be named promiscuous xenobiotic receptor) is a xenobiotic sensor that is directly involved in metabolizing pathways of a wide range of environmental contaminants. Objectives: In the present study, we comparably assess the ability of 51 selected pharmaceuticals, pesticides and emerging contaminants to activate PXRs from polar bears and humans using an in vitro luciferase reporter gene assay. Results: We found that polar bear PXR is activated by a wide range of our test compounds (68%) but has a slightly more narrow ligand specificity than human PXR that was activated by 86% of the 51 test compounds. The majority of the agonists identified (70%) produces a stronger induction of the reporter gene via human PXR than via polar bear PXR, however with some notable and environmentally relevant exceptions. Conclusions: Due to the observed differences in activation of polar bear and human PXRs, exposure of each species to environmental agents is likely to induce biotransformation differently in the two species. Bioinformatics analyses and structural modeling studies suggest that amino acids that are not part of the ligand-binding domain and do not interact with the ligand can modulate receptor activation. - Highlights: • Comparative study of ligand activation of human and polar bear PXRs. • Polar bear PXR is a promiscuous ligand-activated nuclear receptor but less so than human PXR. • Environmental contaminants activate human and polar bear PXRs differently. • Expression and ligand promiscuity indicate that PXR is a xenosensor in polar bears

  5. Regulation of cAMP Responsive Element Binding Protein 3-Like 1 (Creb3l1 Expression by Orphan Nuclear Receptor Nr4a1

    Directory of Open Access Journals (Sweden)

    Michael P. Greenwood

    2017-12-01

    Full Text Available Cyclic AMP (cAMP inducible transcription factor cAMP responsive element binding protein 3 like 1 (Creb3l1 is strongly activated in the hypothalamus in response to hyperosmotic cues such as dehydration (DH. We have recently shown that Creb3l1 expression is upregulated by cAMP pathways in vitro, however the exact mechanisms are not known. Here we show that increasing Creb3l1 transcription by raising cAMP levels in mouse pituitary AtT20 cells automatically initiates cleavage of Creb3l1, leading to a greater abundance of the transcriptionally active N-terminal portion. Inhibiting protein synthesis indicated that de novo protein synthesis of an intermediary transcription factor was required for Creb3l1 induction. Strategic mining of our microarray data from dehydrated rodent hypothalamus revealed four candidates, reduced to two by analysis of acute hyperosmotic-induced transcriptional activation profiles in the hypothalamus, and one, orphan nuclear receptor Nr4a1, by direct shRNA mediated silencing in AtT20 cells. We show that activation of Creb3l1 transcription by Nr4a1 involves interaction with a single NBRE site in the promoter region. The ability to activate Creb3l1 transcription by this pathway in vitro is dictated by the level of methylation of a CpG island within the proximal promoter/5′UTR of this gene. We thus identify a novel cAMP-Nr4a1-Creb3l1 transcriptional pathway in AtT20 cells and also, our evidence would suggest, in the hypothalamus.

  6. Combined experimental and numerical kinetic characterization of NR vulcanized with sulphur, N terbutyl, 2 benzothiazylsulphenamide and N,N diphenyl guanidine

    Energy Technology Data Exchange (ETDEWEB)

    Milani, G., E-mail: gabriele.milani@polimi.it [Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan (Italy); Hanel, T.; Donetti, R. [Pirelli Tyre, Via Alberto e Piero Pirelli 25, 20126 Milan (Italy); Milani, F. [Chem. Co, Via J.F.Kennedy 2, 45030 Occhiobello (Italy)

    2016-06-08

    The paper presents the final results of a comprehensive experimental and numerical analysis aimed at deeply investigating the behavior of Natural Rubber (NR) vulcanized with sulphur in presence of different accelerators during standard rheometer tests. NR in presence of sulphur and two different accelerators (DPG and TBBS) in various concentrations is investigated, changing the curing temperature in the range 150-180°C and obtaining rheometer curves with a step of 10°C. Sulphur-TBBS concentrations considered are 1-1, 1-3, 3-3 and 3-1, with DPG at 1-4 phr respectively. A total of 48 experimental rheometer curves is so obtained. To fit experimental data, the general reaction scheme proposed by Han and co-workers for vulcanized sulphur NR is re-adapted and suitably modified taking into account the single contributions of the different accelerators. Chain reactions initiated by the formation of macro-compounds responsible for the formation of the unmatured crosslinked polymer are accounted for. In presence of two accelerators, reactions are assumed to proceed in parallel, making the practically effective hypothesis that there is no interaction between the two accelerators. From the simplified kinetic scheme adopted, a closed form solution is found for the crosslink density, with the only limitation that the induction period is excluded from computations. For each experimented case on the same blend, reaction kinetic constants provided by the model are utilized to deduce their trend in the Arrhenius space, also outside the temperature range inspected. Rather close linearity is found in the majority of the cases. A comparative analysis is carefully conducted among the constants at the different concentrations of S, TBBS and DPG investigated, allowing a prediction of curing behavior at any vulcanization temperature and with concentrations not experimentally tested, without the need of addition costly experimentation.

  7. Dexmedetomidine attenuates neuropathic pain in chronic constriction injury by suppressing NR2B, NF-κB, and iNOS activation

    Directory of Open Access Journals (Sweden)

    Feng Liang

    2017-05-01

    Full Text Available The effective treatment of patients suffering from neuropathic pain remains challenging. Dexmedetomidine (DEX possesses anti-inflammatory activity. However, the role of DEX in neuropathic pain is still unclear. The aim of the present study was to examine DEX an α2-adrenoceptor agonist could improve pain hypersensitivity and reduce inflammatory in a chronic constriction injury (CCI model of the sciatic nerve in Sprague-Dawley rats. Dex was intrathecally administrated 1-h after operation. The paw mechanical withdrawal threshold (MWT and paw withdrawal thermal latency (PWTL were measured on day 1 before operation and on days 1, 7, 14 and 21 after operation, respectively. On day 21, all the rats were decapitated to collect the L4-6 segments of the spinal cord to examine IL-1, TNF-α, IL-6, NR2B, NF-κB, and iNOS mRNA levels using RT-PCR. The postoperative MWT and PWTL were significantly decreased in CCI, and DEX groups as compared to those before surgery and Sham group (P < 0.05. And DEX reversed this trend (P < 0.05. Interleukin 1 (IL-1, tumor necrosis factor α (TNF-α, IL-6 mRNA expression significantly increased postsurgery in CCI group as compared to that of Sham group (P < 0.05; DEX blocked increased IL-1, TNF-α, IL-6, N-methyl-D-aspartate (NMDA receptor 2B (NR2B, nuclear factor κB (NF-κB, and inducible isoform of nitric oxide synthase (iNOS mRNA levels (P < 0.05. DEX may alleviate neuropathic hypersensitivity and inflammation partially by inhibiting NR2B, NF-κB, and iNOS expression in the spinal cord of rats with neuropathic pain resulting from CCI of the sciatic nerve.

  8. Combined experimental and numerical kinetic characterization of NR vulcanized with sulphur, N terbutyl, 2 benzothiazylsulphenamide and N,N diphenyl guanidine

    International Nuclear Information System (INIS)

    Milani, G.; Hanel, T.; Donetti, R.; Milani, F.

    2016-01-01

    The paper presents the final results of a comprehensive experimental and numerical analysis aimed at deeply investigating the behavior of Natural Rubber (NR) vulcanized with sulphur in presence of different accelerators during standard rheometer tests. NR in presence of sulphur and two different accelerators (DPG and TBBS) in various concentrations is investigated, changing the curing temperature in the range 150-180°C and obtaining rheometer curves with a step of 10°C. Sulphur-TBBS concentrations considered are 1-1, 1-3, 3-3 and 3-1, with DPG at 1-4 phr respectively. A total of 48 experimental rheometer curves is so obtained. To fit experimental data, the general reaction scheme proposed by Han and co-workers for vulcanized sulphur NR is re-adapted and suitably modified taking into account the single contributions of the different accelerators. Chain reactions initiated by the formation of macro-compounds responsible for the formation of the unmatured crosslinked polymer are accounted for. In presence of two accelerators, reactions are assumed to proceed in parallel, making the practically effective hypothesis that there is no interaction between the two accelerators. From the simplified kinetic scheme adopted, a closed form solution is found for the crosslink density, with the only limitation that the induction period is excluded from computations. For each experimented case on the same blend, reaction kinetic constants provided by the model are utilized to deduce their trend in the Arrhenius space, also outside the temperature range inspected. Rather close linearity is found in the majority of the cases. A comparative analysis is carefully conducted among the constants at the different concentrations of S, TBBS and DPG investigated, allowing a prediction of curing behavior at any vulcanization temperature and with concentrations not experimentally tested, without the need of addition costly experimentation.

  9. Nuclear receptor 5A (NR5A) family regulates 5-aminolevulinic acid synthase 1 (ALAS1) gene expression in steroidogenic cells.

    Science.gov (United States)

    Ju, Yunfeng; Mizutani, Tetsuya; Imamichi, Yoshitaka; Yazawa, Takashi; Matsumura, Takehiro; Kawabe, Shinya; Kanno, Masafumi; Umezawa, Akihiro; Kangawa, Kenji; Miyamoto, Kaoru

    2012-11-01

    5-Aminolevulinic acid synthase 1 (ALAS1) is a rate-limiting enzyme for heme biosynthesis in mammals. Heme is essential for the catalytic activities of P450 enzymes including steroid metabolic enzymes. Nuclear receptor 5A (NR5A) family proteins, steroidogenic factor-1 (SF-1), and liver receptor homolog-1 (LRH-1) play pivotal roles in regulation of steroidogenic enzymes. Recently, we showed that expression of SF-1/LRH-1 induces differentiation of mesenchymal stem cells into steroidogenic cells. In this study, genome-wide analysis revealed that ALAS1 was a novel SF-1-target gene in differentiated mesenchymal stem cells. Chromatin immunoprecipitation and reporter assays revealed that SF-1/LRH-1 up-regulated ALAS1 gene transcription in steroidogenic cells via binding to a 3.5-kb upstream region of ALAS1. The ALAS1 gene was up-regulated by overexpression of SF-1/LRH-1 in steroidogenic cells and down-regulated by knockdown of SF-1 in these cells. Peroxisome proliferator-activated receptor-γ coactivator-1α, a coactivator of nuclear receptors, also strongly coactivated expression of NR5A-target genes. Reporter analysis revealed that peroxisome proliferator-activated receptor-γ coactivator-1α strongly augmented ALAS1 gene transcription caused by SF-1 binding to the 3.5-kb upstream region. Finally knockdown of ALAS1 resulted in reduced progesterone production by steroidogenic cells. These results indicate that ALAS1 is a novel NR5A-target gene and participates in steroid hormone production.

  10. Tetrahalide complexes of the [U(NR)2]2+ ion: synthesis, theory, and chlorine K-edge X-ray absorption spectroscopy.

    Science.gov (United States)

    Spencer, Liam P; Yang, Ping; Minasian, Stefan G; Jilek, Robert E; Batista, Enrique R; Boland, Kevin S; Boncella, James M; Conradson, Steven D; Clark, David L; Hayton, Trevor W; Kozimor, Stosh A; Martin, Richard L; MacInnes, Molly M; Olson, Angela C; Scott, Brian L; Shuh, David K; Wilkerson, Marianne P

    2013-02-13

    Synthetic routes to salts containing uranium bis-imido tetrahalide anions [U(NR)(2)X(4)](2-) (X = Cl(-), Br(-)) and non-coordinating NEt(4)(+) and PPh(4)(+) countercations are reported. In general, these compounds can be prepared from U(NR)(2)I(2)(THF)(x) (x = 2 and R = (t)Bu, Ph; x = 3 and R = Me) upon addition of excess halide. In addition to providing stable coordination complexes with Cl(-), the [U(NMe)(2)](2+) cation also reacts with Br(-) to form stable [NEt(4)](2)[U(NMe)(2)Br(4)] complexes. These materials were used as a platform to compare electronic structure and bonding in [U(NR)(2)](2+) with [UO(2)](2+). Specifically, Cl K-edge X-ray absorption spectroscopy (XAS) and both ground-state and time-dependent hybrid density functional theory (DFT and TDDFT) were used to probe U-Cl bonding interactions in [PPh(4)](2)[U(N(t)Bu)(2)Cl(4)] and [PPh(4)](2)[UO(2)Cl(4)]. The DFT and XAS results show the total amount of Cl 3p character mixed with the U 5f orbitals was roughly 7-10% per U-Cl bond for both compounds, which shows that moving from oxo to imido has little effect on orbital mixing between the U 5f and equatorial Cl 3p orbitals. The results are presented in the context of recent Cl K-edge XAS and DFT studies on other hexavalent uranium chloride systems with fewer oxo or imido ligands.

  11. Measurement of extrapolation curves for the secondary pattern of beta radiation Nr. 86 calibrated in rapidity of absorbed dose for tissue equivalent by the Physikalisch Technische Bundesanstalt

    International Nuclear Information System (INIS)

    Alvarez R, J.T.

    1988-10-01

    The following report has as objective to present the obtained results of measuring - with a camera of extrapolation of variable electrodes (CE) - the dose speed absorbed in equivalent fabric given by the group of sources of the secondary pattern of radiation Beta Nr. 86, (PSB), and to compare this results with those presented by the calibration certificates that accompany the PSB extended by the primary laboratory Physikalisch Technische Bundesanstalt, (PTB), of the R.F.A. as well as the uncertainties associated to the measure process. (Author)

  12. Hydrogen-rich saline controls remifentanil-induced hypernociception and NMDA receptor NR1 subunit membrane trafficking through GSK-3β in the DRG in rats.

    Science.gov (United States)

    Zhang, Linlin; Shu, Ruichen; Wang, Chunyan; Wang, Haiyun; Li, Nan; Wang, Guolin

    2014-07-01

    Although NMDAR trafficking mediated by GSK-3β involvement in transmission of pronociceptive messages in the spinal cord has been confirmed by our previous studies, whether NMDAR trafficking is implicated in peripheral sensitization remains equivocal. It is demonstrated that inflammation is associated with spinal NMDAR-containing nociceptive neurons activation and the maintenance of opioid induced pain hypersensitivity. However, whether and how hydrogen-rich saline, as an effective anti-inflammatory drug, could prevent hyperalgesia through affecting peripheral sensitization caused by NMDAR activation remains to be explored. To test these effects, hydrogen-rich saline (2.5, 5 or 10 ml/kg) was administrated intraperitoneally after remifentanil infusion, NMDAR antagonist MK-801 or GSK-3β inhibitor TDZD-8 was administrated intravenously before remifentanil infusion in rats. We examined time course of hydrogen concentration in blood after hydrogen-rich saline administration. Mechanical and thermal hyperalgesia were evaluated by measuring PWT and PWL for 48 post-infusion hours, respectively. Western blotting and real-time qPCR assay were applied to analyze the NR1 membrane trafficking, GSK-3β expression and activity in DRG. Inflammatory mediators (TNF-α, IL-1β, and IL-6) expressions in DRG were also analyzed. We found that NR1 membrane trafficking in DRG increased, possibly due to GSK-3β activation after remifentanil infusion. We also discovered that hydrogen-rich saline not 2.5 ml/kg but 5 and 10 ml/kg could dose-dependently attenuate mechanical and thermal hyperalgesia without affecting baseline nociceptive threshold, reduce expressions of inflammatory mediators (TNF-α, IL-1β, and IL-6) and decrease NR1 trafficking mediated by GSK-3β, and minimal effective concentration was observed to be higher than 10 μmol/L, namely peak concentration in arterial blood after administration of HRS 2.5 ml/kg without any influence on hyperalgesia. Our results indicated that

  13. Rhodium on tungsten carbide - a new catalyst for reforming CH{sub 4} with CO{sub 2}; Rhodium auf Wolframcarbid - ein neuer Katalysator zum Reformieren von CH{sub 4} mit CO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Voelter, J. [Inst. fuer Angewandte Chemie e.V., Berlin (Germany); Berndt, H. [Inst. fuer Angewandte Chemie e.V., Berlin (Germany); Lietz, G. [Inst. fuer Angewandte Chemie e.V., Berlin (Germany); Preiss, H. [TU Bergakademie Freiberg (Germany). Inst. fuer Energieverfahrenstechnik; Tamme, R. [Deutsche Forschungsanstalt fuer Luft- und Raumfahrt e.V., Stuttgart (Germany). Inst. fuer Technische Thermodynamik

    1997-02-01

    bei 600 bis 700 C wieder als Waerme gewonnen werden. Die vorliegende Arbeit betrifft Katalysatoren zum Reformieren im Solarreceiverreaktor. Bisher wurde eine mit dispersem Rhodium geschwaerzte, makroporoese Schaumkeramik sowohl zur Absorption von Solarenergie wie auch zur Aktivierung der Reformierreaktion benutzt. Dabei ist die Menge Rhodium, die fuer eine effektive Absorption der Strahlung eingesetzt werden muss, viel groesser als die Rh-Menge, die fuer die katalytische Reaktion erforderlich ist. Um hochdisperses Rhodium zu erzeugen, wird die Schaumkeramik aus {alpha}-Al{sub 2}O{sub 3} mit einem Washcoat ({gamma}-Al{sub 2}O{sub 3}) beschichtet. Ziel unserer Untersuchungen war es, Rh nur noch als Katalysator einzusetzen und fuer die Strahlungsabsorption ein anderes geeignetes Material zu verwenden. Es sollte untersucht werden, ob das die Strahlung reflektierende {gamma}-Al{sub 2}O{sub 3} durch einen schwarzen poroesen Katalysatortraeger ersetzt werden kann. Als Material, das auch der thermischen Belastung standhalten kann, kam Wolframcarbid (WC) in Frage. WC ist bisher in der Literatur noch nicht als Traeger fuer einen Metallkatalysator beschrieben worden. Fuer die spezielle Anwendung im Receiverreaktor musste untersucht werden, ob es als ein Traeger zur Dispergierung von Rhodium geeignet ist und wie es sich als Washcoat zur Beschichtung der Schaumkeramik verhaelt. (orig./SR)

  14. Analysis of the gene coding for steroidogenic factor 1 (SF1, NR5A1) in a cohort of 50 Egyptian patients with 46,XY disorders of sex development.

    Science.gov (United States)

    Tantawy, Sally; Mazen, Inas; Soliman, Hala; Anwar, Ghada; Atef, Abeer; El-Gammal, Mona; El-Kotoury, Ahmed; Mekkawy, Mona; Torky, Ahmad; Rudolf, Agnes; Schrumpf, Pamela; Grüters, Annette; Krude, Heiko; Dumargne, Marie-Charlotte; Astudillo, Rebekka; Bashamboo, Anu; Biebermann, Heike; Köhler, Birgit

    2014-05-01

    Steroidogenic factor 1 (SF1, NR5A1) is a key transcriptional regulator of genes involved in the hypothalamic-pituitary-gonadal axis. Recently, SF1 mutations were found to be a frequent cause of 46,XY disorders of sex development (DSD) in humans. We investigate the frequency of NR5A1 mutations in an Egyptian cohort of XY DSD. Clinical assessment, endocrine evaluation and genetic analysis of 50 Egyptian XY DSD patients (without adrenal insufficiency) with a wide phenotypic spectrum. Molecular analysis of NR5A1 gene by direct sequencing followed by in vitro functional analysis of the two novel missense mutations detected. Three novel heterozygous mutations of the coding region in patients with hypospadias were detected. p.Glu121AlafsX25 results in severely truncated protein, p.Arg62Cys lies in DNA-binding zinc finger, whereas p.Ala154Thr lies in the hinge region of SF1 protein. Transactivation assays using reporter constructs carrying promoters of anti-Müllerian hormone (AMH), CYP11A1 and TESCO core enhancer of Sox9 showed that p.Ala154Thr and p.Arg62Cys mutations result in aberrant biological activity of NR5A1. A total of 17 patients (34%) harboured the p.Gly146Ala polymorphism. We identified two novel NR5A1 mutations showing impaired function in 23 Egyptian XY DSD patients with hypospadias (8.5%). This is the first study searching for NR5A1 mutations in oriental patients from the Middle East and Arab region with XY DSD and no adrenal insufficiency, revealing a frequency similar to that in European patients (6.5-15%). We recommend screening of NR5A1 in patients with hypospadias and gonadal dysgenesis. Yearly follow-ups of gonadal function and early cryoconservation of sperms should be performed in XY DSD patients with NR5A1 mutations given the risk of future fertility problems due to early gonadal failure.

  15. Untersuchungen zum Potential eines Verbrennungsmotors mit Druckwellenlader

    OpenAIRE

    Binder, Emanuel

    2015-01-01

    Mithilfe der Abgasturboaufladung lässt sich der Kraftstoffverbrauch im Fahrzeugbetrieb durch das Anheben der spezifischen Motorleistung senken. Im Gegensatz zur mechanischen Aufladung verschlechtert sich jedoch mit der Abgasturbolaufladung das Ansprechverhalten. Eine Möglichkeit den Zielkonflikt von Kraftstoffverbrauch und Ansprechverhalten zu lösen ist es, den Abgasturbolader durch einen Druckwellenladers zu ersetzen. Dieser überträgt die Abgasenergie direkt auf die Frischluft in Form von Dr...

  16. Zum 80. Geburtstag der Zeitschrift Germanoslavica

    Czech Academy of Sciences Publication Activity Database

    Ulbrecht, Siegfried

    2011-01-01

    Roč. 17, č. 14 (2011), s. 3 ISSN 1213-2659 Institutional research plan: CEZ:AV0Z90920516 Keywords : Institute of Slavonic Studies sof the ASCR, v.v * scientific journal * Germanoslavica Subject RIV: AJ - Letters, Mass-media, Audiovision

  17. Verfahren zum Herstellen einer Beschichtung eines Substrats

    OpenAIRE

    Wilke, Martin; Töpper, Michael

    2013-01-01

    The method involves applying coating material (7) on surface (2) of recess (3) formed in substrate (1). A liquid auxiliary agent (6) is applied on substrate surface, such that recess is filled with auxiliary agent. The coating material is subsequently applied to auxiliary agent on substrate. A coating material portion in auxiliary agent is transported by coating material diffusion. The agent is subsequently separated from coating material, such that coating material on substrate surface is le...

  18. Glossary of natural resource; Glossar zum Ressourcenschutz

    Energy Technology Data Exchange (ETDEWEB)

    Kosmol, Jan; Kanthak, Judit; Herrmann, Friederike; Golde, Michael; Alsleben, Carsten; Penn-Bressel, Gertrude; Schmitz, Stefan; Gromke, Ulrich

    2012-01-15

    The contribution under consideration presents the most important terms in a logical context, and distinguishes clearly between these technical terms. Thus, this contribution raises the profile of the scientific, political and public debate. The glossary is being developed by an interdisciplinary working group of the Federal Environment Agency (Dessau-Rosslau, Federal Republic of Germany) since 2009. In 2010, the glossary was co-ordinated with parts of the German scientific community.

  19. Vom Webstuhl zum World Wide Web

    Directory of Open Access Journals (Sweden)

    Heide Palmer

    2003-03-01

    Full Text Available Die Autorin ist Direktorin der „Cybernetic Culture Research Unit“ an der University of Warwick. Sie bezeichnet sich als eine der Begründerinnen des „Cyberfeminismus“ und will mit nullen + einsen das Thema Frauen und Computer in ein neues Licht setzen. Ihre zentrale These lautet, dass das Internet eine Weiterentwicklung des Webstuhls sei. Mit Hilfe einer collage-artigen Erzählweise bildet Plant die nicht-lineare Struktur moderner Kommunikationsmittel ab.

  20. Einrichtung zum Spannen eines Endlos-Transmissionselements

    OpenAIRE

    Boley, D.; Rhomberg Kauert, A.

    1995-01-01

    The description refers to a device for clamping an endless transmission element such as a belt, a toothed belt or a chain of a drive unit, comprising a roller which contacts the transmission element and which has a rocker bearing which is applied against a spring in the direction of the transmission element. The invention is characterized by the fact that a blocking unit is provided to prevent the rocker from performing a return stroke.

  1. Das Ausmalbuch zum ATLAS-Experiment

    CERN Multimedia

    Anthony, Katarina

    2017-01-01

    Deutsche Fassung - The ATLAS Experiment Colouring Book is a free-to-download educational book, ideal for kids aged 5-9. It aims to introduce children to the field of High-Energy Physics, as well as the work being carried out by the ATLAS Collaboration.

  2. Vorrichtung und Verfahren zum Schaetzen eines Motordrehmoments

    OpenAIRE

    2004-01-01

    WO2004051212 A UPAB: 20040716 NOVELTY - The method for determining the torque of a combustion engine by measurement (1) of a measurement value representative of an angular velocity of the combustion engine and measurement of a second measurement value representative the charging pressure of the combustion engine or simulation of a charging pressure dependent on the first measurement value. Typically the torque is determined from the engine speed or the average indicated pressure. DETAILED DES...

  3. Vorrichtung zum Erzeugen eines markierten Referenzdatenstroms

    OpenAIRE

    Eilers, D.; Weiß, G.

    2008-01-01

    EP 2194457 A2 UPAB: 20100617 NOVELTY - The device (400) has a reference data generator (110) for producing a reference data stream (120) based on an input data stream (102), where the reference data stream includes a set of partial reference data streams with a set of markings. The markings indicate whether a chronological order between occurrences of the partial reference data streams is prescribed in the reference data stream. An analysis module (420) performs evaluation of a test object (4...

  4. Verfahren zum Herstellen einer kristallinen organischen Halbleiterschicht

    OpenAIRE

    Burghart, Markus

    2010-01-01

    The method involves applying (10) a layer of an organic semiconductor material on a carrier. The organic semiconductor material layer is heated at a temperature, so as to generate (20) recrystallization of organic semiconductor material and for increasing the crystallinity of the organic semiconductor material. A cooling down layer is formed such that the raised crystallinity of the organic semiconductor material is maintained. The organic semiconductor material is applied to the region of la...

  5. Partiell oberflaechengehaertete Riffelwalze und Verfahren zum Oberflaechenhaerten

    OpenAIRE

    Paul, H.; Krumphold, R.; Pollack, D.; Burck, P.; Kraegelin, L.; Neubert, P.

    1997-01-01

    DE 3711450 A UPAB: 19970307 Worn top surfaces of grooved rolls are renewed by producing a hard area (2) having a max. thickness of 0.4 mm and a width (3) determined by the tangent to the grooved surface from a plane cutting across the top of the groove equal to 45 deg.. The wear experienced by the roller corresponds to the shape of the hardened area therefore any soft annealing prior to machining is avoided. The energy beam used for hardening is pref. a CO2 laser and the ridge area is axially...

  6. Kontingenz, Redundanz, Transzendenz? - Zum Gerechtigkeitsbegriff Niklas Luhmanns

    Directory of Open Access Journals (Sweden)

    Moritz Renner

    2008-11-01

    Full Text Available Niklas Luhmanns systemtheoretische Rekonstruktion der „Kontingenzformel Gerechtigkeit“ gilt als der anspruchsvollste Versuch einer positivistischen Gerechtigkeitstheorie. Der Versuch zielt darauf ab, den Begriff der Gerechtigkeit aus den Operationen des Rechtssystems selbst zu entwickeln und setzt an der formalen Konsistenz rechtlicher Entscheidungen an. Vielfach ist Luhmanns Theorie daher ihr vermeintlicher Verlust der Einzelfallgerechtigkeit vorgeworfen worden. Neuere, an der Dekonstruktion orientierte Luhmann-Interpretationen begegnen diesem Einwand, indem sie die Offenlegung der Selbstwidersprüche des selbstreferentiellen Rechtssystems als eine Form der Rechtskritik auffassen, welche dem Rechtsdiskurs zwar immanent bleibt, zugleich aber auf die Möglichkeit seiner Transzendierung verweist.

  7. Kurz zum Klima: Schiefergas und Fracking

    OpenAIRE

    Triebswetter, Ursula; Lippelt, Jana

    2012-01-01

    Das Verfahren zur Gewinnung von Erdgas aus wenig durchlässigem Gestein, das in den USA erfundene und dort bereits weit verbreitete »Hydraulic Fracturing«, kurz Fracking, ist aus Umweltgründen umstritten. Der Beitrag skizziert zunächst die Technologie des Fracking, umreißt dann die weltweiten Lagerstätten und beschreibt die umweltpolitische Problematik. Abschließend wird die aktuelle Situation in Deutschland dargestellt.

  8. Zum Stand und den Perspektiven der allgemeinsprachlichen ...

    African Journals Online (AJOL)

    Mev. R.B. Ruthven

    so dass man nicht weiß, wie die Aktualität der gegenwärtigen slowenischen ...... zu wa23 in Abb. 1 gehören; Abkürzungen: A. = ABSTRAKTE; H = HIERAR-. CHISCHE ...... Ulrich, Stefan Evert, Egbert Lehmann und Christian Rohrer (Hrsg.).

  9. Verfahren zum Herstellen einer wasserfreien Siliziumdioxidschicht

    OpenAIRE

    Klumpp, A.

    1991-01-01

    In a process for the production of a moistureless silicon dioxide layer starting from a silicate layer, polysiloxane is photoinduced, polymerized and separated, whereupon the polysiloxane layer is converted into a silicate layer. By transforming the the silicate layer by chemical reaction of the water bound in said layer by means of silane, the silicate layer is converted into a moistureless silicon dioxide layer.

  10. SMED-TLX-1 (NR2E1) is critical for tissue and body plan maintenance in Schmidtea mediterranea in fasting/feeding cycles.

    Science.gov (United States)

    Raška, O; Kostrouchová, V; Behenský, F; Yilma, P; Saudek, V; Kostrouch, Z; Kostrouchová, M

    2011-01-01

    Nuclear receptors (NRs), or nuclear hormone receptors (NHRs), are transcription factors that regulate development and metabolism of most if not all animal species. Their regulatory networks include conserved mechanisms that are shared in-between species as well as mechanisms that are restricted to certain phyla or even species. In search for conserved members of the NHR family in Schmidtea mediterranea, we identified a molecular signature of a class of NRs, NR2E1, in the S. mediterranea genome and cloned its complete cDNA coding sequence. The derived amino acid sequence shows a high degree of conservation of both DNA-binding domain and ligand- binding domain and a remarkably high homology to vertebrate NR2E1 and C. elegans NHR-67. Quantitative PCR detected approximately ten-fold higher expression of Smed-tlx-1 in the proximal part of the head compared to the tail region. The expression of Smed-tlx-1 is higher during fed state than during fasting. Smed-tlx-1 down-regulation by RNA interference affects the ability of the animals to maintain body plan and induces defects of brain, eyes and body shape during fasting and re-growing cycles. These results suggest that SMED-TLX-1 is critical for tissue and body plan maintenance in planaria.

  11. Effects of Rice Husk Modification with Liquid Natural Rubber and Exposure to Electron Beam Radiation on the Mechanical Properties of NR/ HDPE/ Rice Husk Composites

    International Nuclear Information System (INIS)

    Lane, C.E.; Ishak Ahmad; Ibrahim Abdullah; Dahlan Mohd

    2011-01-01

    Rice husk (RH) powder is a natural fibre capable of reinforcing natural rubber thermoplastic (TPNR) NR/ HDPE composites on specific modification of the particle surface. In this study the modification of RH powder involved pre-treatment with 5 % sodium hydroxide (NaOH) solution, soaking in LNR solution and exposure of LNR coated RH to electron beam (EB) irradiation. Preparation of NR/ HDPE/ RH composites was via melt-mixing in an internal mixer at predetermined conditions. Morphology study of the composites using scanning electron microscope (SEM) showed a homogeneous distribution of modified RH particles and particle-matrix interaction in the composite. Modified RH filled composites exhibited a significant change in mechanical properties. The maximum stress and impact strength were 6.7 MPa and 13.2 kJ/ m 2 , respectively at 20 kGy radiation, while the tensile modulus was 79 MPa at 30 kGy dose. The interfacial RH-TPNR interaction for the LNR-EB treated RH particles had improved in the EB dosage range of 20-30 kGy. However, over exposure to radiation caused degradation of rubber coat and interaction between particles to increase. Agglomeration of filler particles would occur and caused inhomogeneous distribution of filler in the composite. (author)

  12. The Effect Of Gamma Irradiation And CaCO3 On The Physical And Mechanical Properties Of Natural Rubber (NR) Compound

    International Nuclear Information System (INIS)

    Iskandar, Sudrajat; Marliyanti, Isni; Kadariyah; Kardha, Made Sumarti

    2000-01-01

    The characterization of the effect of gamma irradiation and CaCO 3 on the physical and mechanical properties of NR compound (NRC) have been started. The NRC was made by blending of NR and low density polyethylene (LDPE), carbon black, additives (stearic acid, ZnO, irganox 1076, paraffin wax and minarex B oil) in certain amount and CaCO 3 with various composition of 33,67 and 100 phr (part per hundred ratio of rubber) using hot roll mill at 120 deg C and 100 deg C of front and back roll respectively. For making film, film, the NRC was then pressed by hot and cold press machine at 135 deg C and at room temperature respectively for about 3 minutes. The film was then irradiated with gamma rays at 150, 300, and 500 kGy. Characterization was done according to the Indonesian National Standard (SNI) The experimental results showed that by increasing the irradiation dose, the aging resistance, tensile strength, elongation at break, solvent xylene resistance, tear strength and hardness of natural rubber compound increased. While by increasing the CaCO 3 content, the hardness and specific gravity of irradiated NRC. Were increased. But the tensile strength, elongation at break and tear strength of irradiated NRC decreased According to SNI no 12-1000-1989 and SII 0944-84 that the experiment results may possible to used for making shoe sole and rubber carpet

  13. Mechanical properties of chemically modified Sansevieria trifasciata/natural rubber/high density polyethylene (STF/NR/HDPE) composites: Effect of silane coupling agent

    Science.gov (United States)

    Zakaria, Nurzam Ezdiani; Baharum, Azizah; Ahmad, Ishak

    2018-04-01

    The main objective of this research is to study the effects of chemical modification on the mechanical properties of treated Sansevieria trifasciata fiber/natural rubber/high density polyethylene (TSTF/NR/HDPE) composites. Processing of STF/NR/HDPE composites was done by using an internal mixer. The processing parameters used were 135°C for temperature and a mixing rotor speed of 55 rpm for 15 minutes. Filler loading was varied from 10% to 40% of STF and the fiber size used was 125 µm. The composite blends obtained then were pressed with a hot press machine to get test samples of 1 mm and 3 mm of thickness. Samples were evaluated via tensile tests, Izod impact test and scanning electron microscopy (SEM). Results showed that tensile strength and strain value decreased while tensile modulus increased when filler loading increased. Impact strength increased when filler loading increased and began to decrease after 10% of filler amount for treated composites. For untreated composites, impact strength began to decrease after 20% of filler loading. Chemical modification by using silane coupling agent has improved certain mechanical properties of the composites such as tensile strength, strain value and tensile modulus. Adding more amount of filler will also increase the viscosity and the stiffness of the materials.

  14. The sex-dependent role of the glucocorticoid receptor in depression: variations in the NR3C1 gene are associated with major depressive disorder in women but not in men.

    Science.gov (United States)

    Sarubin, Nina; Hilbert, Sven; Naumann, Felix; Zill, Peter; Wimmer, Anna-Maria; Nothdurfter, Caroline; Rupprecht, Rainer; Baghai, Thomas C; Bühner, Markus; Schüle, Cornelius

    2017-03-01

    Genetic variations in the glucocorticoid receptor (GR) and the mineralocorticoid receptor (MR) have been associated with maladaptive stress responses and major depressive disorder (MDD). In a case-control study design, we examined whether single nucleotide polymorphisms (SNPs) and haploid genotype (haplotype) associations of MR gene NR3C2, GR gene NR3C1 and genes of GR chaperone molecules FK506 binding protein 5 (FKBP5) and corticotrophin-releasing hormone receptor 1 (CRHR1) differed between healthy subjects (n = 634) and inpatients with major depressive disorder (n = 412). All analyses were conducted for women and men separately. After conservative correction of Type-I-error to obtain reliable p values, one SNP in the NR3C1 gene, namely rs6195, showed a significant association with the presence of a major depression (p = 0.048) in females. In contrast, NR3C2, FKBP5 and CRHR1 polymorphisms were not significantly associated with MDD. No haplotype effects could be identified. Our results support the notion of an association between variants of GR-related genes in women and the pathophysiology of depression: females suffering from MDD showed a more than three times higher frequency of the T/C polymorphism compared to controls, which thus seems to increase the vulnerability to depression in females.

  15. [Johan Engström. Revals Befästningsdhistoria sedd utifrån en maquett från 1682. Statens försvarshistoriska museer 2011. Skrift nr 17. ] / Ragnar Nurk, Robert Treufeld

    Index Scriptorium Estoniae

    Nurk, Ragnar

    2014-01-01

    Arvustus: Johan Engström. Revals Befästningsdhistoria sedd utifrån en maquett från 1682. Statens försvarshistoriska museer 2011. Skrift nr 17. 2011. Rootsi-aegsestest kindlustustest. Hilisema perioodi kindlustustest

  16. Shikonin, a constituent of Lithospermum erythrorhizon exhibits anti-allergic effects by suppressing orphan nuclear receptor Nr4a family gene expression as a new prototype of calcineurin inhibitors in mast cells.

    Science.gov (United States)

    Wang, Xiaoyu; Hayashi, Shusaku; Umezaki, Masahito; Yamamoto, Takeshi; Kageyama-Yahara, Natsuko; Kondo, Takashi; Kadowaki, Makoto

    2014-12-05

    Over the last few decades, food allergy (FA) has become a common disease in infants in advanced countries. However, anti-allergic medicines available in the market have no effect on FA, and consequently effective drug therapies for FA are not yet available. We have already demonstrated that mucosal mast cells play an essential role in the development of FA in a murine model. Thus, we screened many constituents from medicinal herbs for the ability to inhibit rat basophilic leukemia-2H3 mast-like cell degranulation, and found that shikonin, a naphthoquinone dye from Lithospermum erythrorhizon, exhibited the most potent inhibitory effect among them. Furthermore, shikonin extremely inhibited the IgE/antigen-induced and calcium ionophore-induced upregulation of tumor necrosis factor (TNF)-α mRNA expression in mucosal-type bone marrow-derived mast cells (mBMMCs). Global gene expression analysis confirmed by real-time PCR revealed that shikonin drastically inhibited the IgE/antigen-induced and calcium ionophore-induced upregulation of mRNA expression of the nuclear orphan receptor 4a family (Nr4a1, Nr4a2 and Nr4a3) in mBMMCs, and knockdown of Nr4a1 or Nr4a2 suppressed the IgE/antigen-induced upregulation of TNF-α mRNA expression. Computational docking simulation of a small molecule for a target protein is a useful technique to elucidate the molecular mechanisms underlying the effects of drugs. Therefore, the simulation revealed that the predicted binding sites of shikonin to immunophilins (cyclophilin A and FK506 binding protein (FKBP) 12) were almost the same as the binding sites of immunosuppressants (cyclosporin A and FK506) to immunophilins. Indeed, shikonin inhibited the calcineurin activity to a similar extent as cyclosporin A that markedly suppressed the IgE/antigen-enhanced mRNA expression of TNF-α and the Nr4a family in mBMMCs. These findings suggest that shikonin suppresses mucosal mast cell activation by reducing Nr4a family gene expression through the

  17. Slagmark nr. 60

    DEFF Research Database (Denmark)

    historieundervisning, danske politikeres brug af fortællingen om besættelsestiden, historiepolitik i Rwanda og Eksjugoslavien, holocaustmindesmærker, erstatningskrav for historiske uretfærdigheder, erindringspolitik i Helsingfors’ byrum, og danske, britiske og amerikanske historikeres forhold til historiepolitik....

  18. Der Einsatz von medizinischen Trainingszentren für die Ausbildung zum Arzt in Deutschland, Österreich und der deutschsprachigen Schweiz [Clinical Skills Labs in Medical Education in Germany, Austria and German Speaking Switzerland

    Directory of Open Access Journals (Sweden)

    Schmidt, Anita

    2008-05-01

    . This would facilitate the development of common quality standards and guidelines for medical skills labs. It also seems to be necessary to define the term “central skills lab” accurately. The authors recommend the Committee on Practical Skills of the Gesellschaft für Medizinische Ausbildung (GMA as a platform for such co-operation. [german] Zielsetzung: Seit der Novellierung der ÄAppO im Jahr 2002 werden im deutschsprachigen Raum an den medizinischen Universitäten zunehmend Medizinische Trainings- und Prüfungszentren eingerichtet. Die vorliegende Erhebung soll einen Überblick über den Stand der Entwicklung in diesem Bereich in Deutschland, Österreich und der deutschsprachigen Schweiz bieten. Methodik: In der Zeit von August bis Dezember 2007 wurden alle 43 medizinischen Fakultäten in Deutschland, Österreich und der deutschsprachigen Schweiz schriftlich befragt. Der Fragebogen umfasste 7 Themenbereiche. Die weitere Auswertung bezieht sich auf Trainingszentren, in denen mehr als eine Disziplin unterrichtet wird („zentrale“ Trainingszentren. Ergebnisse: Der Rücklauf betrug 100%. Von 43 Fakultäten verfügten 33 zum Zeitpunkt der Befragung über ein Trainingszentrum, 24 davon gingen in die weitere Auswertung ein. 22 Trainingszentren waren ärztlich geleitet, die Zahl der Mitarbeiter lag zwischen 1 bis 8, die der studentischen Tutoren zwischen 0 und 40. Das Spektrum der zur Verfügung stehenden Grundfläche reichte von unter 100 Quadratmetern bis zu 2500 Quadratmetern. An den meisten Trainingszentren wurde mit strukturierten Arbeitsanleitungen gearbeitet (19 Einrichtungen, in 18 Einrichtungen unterrichteten Ärzte und studentische Tutoren. In der Mehrheit der Fälle (13 war der Unterricht curricular integriert. An 19 Zentren wurden OSKE durchgeführt. An 19 Zentren wurde mit Simulationspatienten gearbeitet, die in 15 Zentren auch für Prüfungen eingesetzt wurden. 17 Zentren arbeiteten mit problemorientiertem Lernen, 16 setzten computerbasiertes Lernen ein

  19. Synthesis and preclinical evaluation of carbon-11 labelled N-((5-(4-fluoro-2-[11C]methoxyphenyl)pyridin-3-yl)methyl)cyclopentanamine as a PET tracer for NR2B subunit-containing NMDA receptors

    International Nuclear Information System (INIS)

    Christiaans, Johannes A.M.; Klein, Pieter J.; Metaxas, Athanasios; Kooijman, Esther J.M.; Schuit, Robert C.; Leysen, Josée E.; Lammertsma, Adriaan A.; Berckel, Bart N.M. van; Windhorst, Albert D.

    2014-01-01

    Introduction: The N-methyl-D-Aspartate (NMDA) receptor plays an important role in learning and memory. Overactivation is thought to play an important role in neurodegenerative disorders such as Alzheimer's disease. Currently, it is not possible to assess N-methyl-D-aspartate receptor (NMDAr) bio-availability in vivo. The purpose of this study was to develop a positron emission tomography (PET) ligand for the NR2B binding site of the NMDA receptor. Methods: N-((5-(4-fluoro-2-methoxyphenyl)pyridin-3-yl)methyl)cyclopentanamine was radiolabelled with carbon-11 in the phenyl moiety. Biodistribution and blocking studies were carried out in anaesthetized mice and in non-anaesthetized rats. Results: N-((5-(4-fluoro-2-[ 11 C]methoxyphenyl)pyridin-3-yl)methyl)cyclopentanamine was prepared in 49 ± 3% (decay-corrected) yield, affording 4.1 ± 0.3 GBq of formulated product at the end of synthesis with a radiochemical purity of > 99% and with a specific activity of 78 ± 10 GBq/μmol. Conclusion: A new NR2B PET ligand was developed in high yield. [ 11 C]4 readily enters the brain and binds to the NR2B subunit-containing NMDAr in the rodent brain. High sigma-1 receptor binding may, however, limit its future application as a PET probe for imaging the NR2B subunit-containing NMDAr. Anaesthesia has an effect on NMDAr function and therefore can complicate interpretation of preclinical in vivo results. In addition, effects of endogenous compounds cannot be excluded. Despite these potential limitations, further studies are warranted to investigate the values of [ 11 C]4 as an NR2B PET ligand

  20. Nitrate ({sup 13}NO{sub 3}{sup -}) flux studies and response to tungstate treatments in wild type barley and in an NR-deficient mutant

    Energy Technology Data Exchange (ETDEWEB)

    Wieneke, J.

    1994-07-01

    Hydroponic experiments were conducted with seedlings of barley (Hordeum vulgare L.), comparing the wild type and an NR-deficient mutant (AZ 12/70), to monitor the NO{sub 3}{sup -} influx/efflux relations and the response to tungstate treatment using {sup 13}N-labelling. Upon treatment with the arginyl-residue-binding inhibitor phenylglyoxal both genotypes responded with an immediate depression of NO{sub 3}{sup -} influx. At low external NO{sub 3}{sup -} concentrations efflux was small (5-15% of the influx) and did not differ substantially between the two genotypes. Although the mutant was distinguished from the wild type by a thinner root system and a reduced shoot length, the total N distribution between roots and shoots and the N concentrations in the root and shoot tissues were fairly comparable. Substantially higher extractable NO{sub 3}{sup -} concentration in the shoots (but not roots) of the mutant may indicate that the capacity to reduce NO{sub 3}{sup -} was restricted due to the very low but still detectable NR activity in the root and shoot tissue. Nevertheless, the mutant must have had supplementary means of assimilating considerable amounts of NO{sub 3}{sup -} over the experimental growth period. At the induced stage, both barley genotypes responded to tungstate treatments with a comparable but not complete depression of the NO{sub 3}{sup -} influx. Part of the NO{sub 3}{sup -} influx appears to be independent of the function of NR since an acceleration of the NO{sub 3}{sup -} uptake capacity to almost half the level of the controls occurred in both cultivars upon induction in spite of pretreatment with 150 micromolar WO{sub 4}{sup 2-} in a molybdenum-free solution prior to NO{sub 3}{sup -} induction. However, 600 micromolar tungstate treatment during the induction phase reduced NO{sub 3}{sup -} influx further (below 20%), but the plants of both cultivars were still able to recover almost completely. (author)

  1. Misturas NR/SBR: influência da composição e do modo de preparação sobre propriedades mecânicas e reométricas NR/SBR mixtures: influence of composition and preparation mode on the mechanical and rheological properties

    Directory of Open Access Journals (Sweden)

    Hérlon M. B. Abreu Junior

    2010-01-01

    Full Text Available Aplicações de produtos à base de borracha diversas vezes requerem um conjunto de propriedades as quais, geralmente, não são fornecidas por um único elastômero. Dessa forma, um dos recursos utilizados é a mistura de dois ou mais polímeros para obtenção de novas propriedades. Neste caso, os demais ingredientes que são adicionados, normalmente distribuem-se de forma irregular, dependendo de sua afinidade com cada uma das borrachas. O grau de dispersão de cada um desses ingredientes em cada fase elastomérica irá então influenciar a velocidade de cura e o grau de vulcanização e, certamente, terá conseqüências nas propriedades do produto final. Neste trabalho, a borracha natural (NR foi misturada ao elastômero de butadieno-estireno (SBR nas proporções de 75:25 e 50:50, em massa. As composições foram preparadas empregando-se quatro maneiras distintas para a incorporação dos aditivos. Os resultados das propriedades mecânicas mostraram que, apesar da utilização de uma mesma formulação, o modo de preparo tem grande influência sobre as propriedades. No caso da mistura NR/SBR as melhores propriedades são obtidas quando é favorecida a vulcanização da borracha que, isoladamente, apresenta propriedades inferiores.Frequently a set of properties required for a given application cannot be accomplished by one elastomer only and, thus, mixtures of two or more polymers must be employed. In these cases, the other ingredients added are normally dispersed in a non homogeneous way, depending on their affinity to each polymer. The degree of dispersion each additive experiences in each elastomeric phase will influence the rate and the degree of vulcanization and will certainly have consequences on the properties of the final product. In this work, natural rubber (NR was mixed with styrene-butadiene elastomer (SBR in 75:25 and 50:50 w/w ratios. The compositions were prepared in a two-roll mill, by introducing the additives according to

  2. Measurement of extrapolation curves for the secondary pattern of beta radiation Nr. 86 calibrated in rapidity of absorbed dose for tissue equivalent by the Physikalisch Technische Bundesanstalt; Medicion de curvas de extrapolacion para el patron secundario de radiacion beta Nr. 86 calibrado en rapidez de dosis absorbida para tejido equivalente por el Physikalisch Technische Bundesanstalt

    Energy Technology Data Exchange (ETDEWEB)

    Alvarez R, J.T

    1988-10-15

    The following report has as objective to present the obtained results of measuring - with a camera of extrapolation of variable electrodes (CE) - the dose speed absorbed in equivalent fabric given by the group of sources of the secondary pattern of radiation Beta Nr. 86, (PSB), and to compare this results with those presented by the calibration certificates that accompany the PSB extended by the primary laboratory Physikalisch Technische Bundesanstalt, (PTB), of the R.F.A. as well as the uncertainties associated to the measure process. (Author)

  3. A neuroligin-1-derived peptide stimulates phosphorylation of the NMDA receptor NR1 subunit and rescues MK-801-induced decrease in long-term potentiation and memory impairment

    DEFF Research Database (Denmark)

    Korshunova, Irina; Gjørlund, Michelle D; Jacobsen, Sylwia Owczarek

    2015-01-01

    neurolide-1 effects on short- and long-term social and spatial memory in social recognition, Morris water-maze, and Y-maze tests. We found that subcutaneous neurolide-1 administration, restored hippocampal LTP compromised by NMDA receptor inhibitor MK-801. It counteracted MK-801-induced memory deficit...... in the water-maze and Y-maze tests after long-term treatment (24 h and 1-2 h before the test), but not after short-term exposure (1-2 h). Long-term exposure to neurolide-1 also facilitated social recognition memory. In addition, neurolide-1-induced phosphorylation of the NMDA receptor NR1 subunit on a site...... receptor phosphorylation after treatment with NL1 or a mimetic peptide, neurolide-1, was quantified by immunoblotting. Subsequently, we investigated effects of neurolide-1 on long-term potentiation (LTP) induction in hippocampal slices compromised by NMDA receptor inhibitor MK-801. Finally, we investigated...

  4. Loss of Function of the Nuclear Receptor NR2F2, Encoding COUP-TF2, Causes Testis Development and Cardiac Defects in 46,XX Children

    DEFF Research Database (Denmark)

    Bashamboo, Anu; Eozenou, Caroline; Jorgensen, Anne

    2018-01-01

    Emerging evidence from murine studies suggests that mammalian sex determination is the outcome of an imbalance between mutually antagonistic male and female regulatory networks that canalize development down one pathway while actively repressing the other. However, in contrast to testis formation......, the gene regulatory pathways governing mammalian ovary development have remained elusive. We performed exome or Sanger sequencing on 79 46,XX SRY-negative individuals with either unexplained virilization or with testicular/ovotesticular disorders/differences of sex development (TDSD/OTDSD). We identified...... tissue. We demonstrate a highly significant association between the NR2F2 loss-of-function mutations and this syndromic form of DSD (p = 2.44 × 10-8). We show that COUP-TF2 is highly abundant in a FOXL2-negative stromal cell population of the fetal human ovary. In contrast to the mouse, these data...

  5. Furfural Production from d-Xylose and Xylan by Using Stable Nafion NR50 and NaCl in a Microwave-Assisted Biphasic Reaction

    Directory of Open Access Journals (Sweden)

    Sarah Le Guenic

    2016-08-01

    Full Text Available Pentose dehydration and direct transformation of xylan into furfural were performed in a water-cyclopentyl methyl ether (CPME biphasic system under microwave irradiation. Heated up between 170 and 190 °C in the presence of Nafion NR50 and NaCl, d-xylose, l-arabinose and xylan gave furfural with maximum yields of 80%, 42% and 55%, respectively. The influence of temperature and reaction time on the reaction kinetics was discussed. This study was also completed by the survey of different reactant ratios, such as organic layer-water or catalyst-inorganic salt ratios. The exchange between proton and cation induced by an excess of NaCl was monitored, and a synergetic effect between the remaining protons and the released HCl was also discovered.

  6. Furfural Production from d-Xylose and Xylan by Using Stable Nafion NR50 and NaCl in a Microwave-Assisted Biphasic Reaction.

    Science.gov (United States)

    Le Guenic, Sarah; Gergela, David; Ceballos, Claire; Delbecq, Frederic; Len, Christophe

    2016-08-22

    Pentose dehydration and direct transformation of xylan into furfural were performed in a water-cyclopentyl methyl ether (CPME) biphasic system under microwave irradiation. Heated up between 170 and 190 °C in the presence of Nafion NR50 and NaCl, d-xylose, l-arabinose and xylan gave furfural with maximum yields of 80%, 42% and 55%, respectively. The influence of temperature and reaction time on the reaction kinetics was discussed. This study was also completed by the survey of different reactant ratios, such as organic layer-water or catalyst-inorganic salt ratios. The exchange between proton and cation induced by an excess of NaCl was monitored, and a synergetic effect between the remaining protons and the released HCl was also discovered.

  7. Evaluation of the effect of the partial substituttion of vanadium and molybdenum by niobium in a tool steel DIN W. NR. 1.2714

    International Nuclear Information System (INIS)

    Isore, A.; Pereira, O.J.; Aleixo, C.

    1982-01-01

    A comparative study of the properties of a steel DIN W. Nr 1.2714 and those of two steels of the same type but with lower amounts of molybdenum and vanadium, higher amounts of silicon and chromium, and with the adition of niobium (0,08 and 0,17%), was carried out. In the three steels with niobium there are niobium carbides wich are aligned in the hot deformation direction, and do not dissolve at the normal austenitizing temperatures. In the steel with higher niobium content, these carbides affect the transverse toughness and its hot ductility (200 - 600 0 C), although sensibly improving the abrasion wear resistance. The results of the fracture toughness and Charpy test, suggest that the niobium carbides do not decrease the crack nucleation resistance. (Author) [pt

  8. Regulation of C. elegans fat uptake and storage by acyl-CoA synthase-3 is dependent on NR5A family nuclear hormone receptor nhr-25

    DEFF Research Database (Denmark)

    Mullaney, Brendan C; Blind, Raymond D; Lemieux, George A

    2010-01-01

    Acyl-CoA synthases are important for lipid synthesis and breakdown, generation of signaling molecules, and lipid modification of proteins, highlighting the challenge of understanding metabolic pathways within intact organisms. From a C. elegans mutagenesis screen, we found that loss of ACS-3...... mutant phenotypes require the nuclear hormone receptor NHR-25, a key regulator of C. elegans molting. Our findings suggest that ACS-3-derived long-chain fatty acyl-CoAs, perhaps incorporated into complex ligands such as phosphoinositides, modulate NHR-25 function, which in turn regulates an endocrine...... program of lipid uptake and synthesis. These results reveal a link between acyl-CoA synthase function and an NR5A family nuclear receptor in C. elegans....

  9. Brain-derived neurotrophic factor (BDNF) in the rostral anterior cingulate cortex (rACC) contributes to neuropathic spontaneous pain-related aversion via NR2B receptors.

    Science.gov (United States)

    Zhang, Le; Wang, Gongming; Ma, Jinben; Liu, Chengxiao; Liu, Xijiang; Zhan, Yufeng; Zhang, Mengyuan

    2016-10-01

    The rostral anterior cingulate cortex (rACC) plays an important role in pain affect. Previous investigations have reported that the rACC mediates the negative affective component of inflammatory pain and contributed to the aversive state of nerve injury-induced neuropathic pain. Brain-derived neurotrophic factor (BDNF), an activity-dependent neuromodulator in the adult brain, is believed to play a role in the development and maintenance of inflammatory and neuropathic pain in the spinal cord. However, whether and how BDNF in the rACC regulates pain-related aversion due to peripheral nerve injury is largely unknown. Behaviorally, using conditioned place preference (CPP) training in rats, which is thought to reveal spontaneous pain-related aversion, we found that CPP was acquired following spinal clonidine in rats with partial sciatic nerve transection. Importantly, BDNF was upregulated within the rACC in of rats with nerve injury and enhanced the CPP acquisition, while a local injection of a BDNF-tropomyosin receptor kinase B (TrkB) antagonist into the rACC completely blocked this process. Finally, we demonstrated that the BDNF/TrkB pathway exerted its function by activating the NR2B receptor, which is widely accepted to be a crucial factor contributing to pain affect. In conclusion, our results demonstrate that the BDNF/TrkB-mediated signaling pathway in the rACC is involved in the development of neuropathic spontaneous pain-related aversion and that this process is dependent upon activation of NR2B receptors. These findings suggest that suppression of the BDNF-related signaling pathway in the rACC may provide a novel strategy to overcome pain-related aversion. Copyright © 2016 Elsevier Inc. All rights reserved.

  10. Blockade of NMDA receptor subtype NR2B prevents seizures but not apoptosis of dentate gyrus neurons in bacterial meningitis in infant rats

    Directory of Open Access Journals (Sweden)

    Täuber Martin G

    2003-09-01

    Full Text Available Abstract Background Excitotoxic neuronal injury by action of the glutamate receptors of the N-methyl-d-aspartate (NMDA subtype have been implicated in the pathogenesis of brain damage as a consequence of bacterial meningitis. The most potent and selective blocker of NMDA receptors containing the NR2B subunit is (R,S-alpha-(4-hydroxyphenyl-beta-methyl-4-(phenylmethyl-1-piperid inepropanol (RO 25-6981. Here we evaluated the effect of RO 25-6981 on hippocampal neuronal apoptosis in an infant rat model of meningitis due to Streptococcus pneumoniae. Animals were randomized for treatment with RO 25-6981 at a dosage of either 0.375 mg (15 mg/kg; n = 28 or 3.75 mg (150 mg/kg; n = 15 every 3 h or an equal volume of sterile saline (250 μl; n = 40 starting at 12 h after infection. Eighteen hours after infection, animals were assessed clinically and seizures were observed for a period of 2 h. At 24 h after infection animals were sacrificed and brains were examined for apoptotic injury to the dentate granule cell layer of the hippocampus. Results Treatment with RO 25-6981 had no effect on clinical scores, but the incidence of seizures was reduced (P Conclusions Treatment with a highly selective blocker of NMDA receptors containing the NR2B subunit failed to protect hippocampal neurons from injury in this model of pneumococcal meningitis, while it had some beneficial effect on the incidence of seizures.

  11. Clinical utility of circulating anti-N-methyl-d-aspartate receptor subunits NR2A/B antibody for the diagnosis of neuropsychiatric syndromes in systemic lupus erythematosus and Sjögren's syndrome: An updated meta-analysis.

    Science.gov (United States)

    Tay, Sen Hee; Fairhurst, Anna-Marie; Mak, Anselm

    2017-02-01

    Neuropsychiatric (NP) events are found in patients with rheumatic diseases, commonly in systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). The standard nomenclature and case definitions for 19 NPSLE syndromes by the American College of Rheumatology (ACR) Committee on Research cover a wide range of NP events seen in both SLE and SS. Despite advances in the understanding of SLE and SS, NP syndromes continue to pose diagnostic challenges. Correct attribution of NP events is critical in determining the correct treatment and prognosis. Anti-N-methyl- d -aspartate receptor subunits NR2A/B (anti-NR2A/B) antibodies have been demonstrated in the sera of SLE and SS patients and have been associated with collective or specific NP syndromes, though not consistently. Interpretation of anti-NR2A/B antibody data in the medical literature is rendered difficult by small sample size of patient groups. By combining different studies to generate a pooled effect size, a meta-analysis can increase the power to detect differences in the presence or absence of NP syndromes. Hence, we set out to perform a meta-analysis to assess the association between anti-NR2A/B antibodies and NP syndromes in SLE and SS. A literature search was conducted using PubMed and other databases from inception to June 2016. We abstracted data relating to anti-NR2A/B antibodies from the identified studies. The random effects model was used to calculate overall combined odds ratio (OD) with its corresponding 95% confidence interval (CI) to evaluate the relationship between anti-NR2A/B antibodies and NP syndromes in SLE and SS patients with and without NP events. We also included our own cohort of 57 SLE patients fulfilling the ACR 1997 revised classification criteria and 58 healthy controls (HCs). In total, 17 studies with data on anti-NR2A/B antibodies in 2212 SLE patients, 66 SS patients, 99 disease controls (DCs) (e.g. antiphospholipid syndrome, myasthenia gravis and autoimmune polyendocrine

  12. Evaluation of load case ``switch-off of the high pressure pump of the emergency core cooling system``, measures of verification and in situ-test; Einstufung des Lastfalls ``Ausfall der TH-Hochdruckeinspeisepumpe``, Massnahmen zur Verifikation bis hin zum Grossversuch

    Energy Technology Data Exchange (ETDEWEB)

    Trobitz, M.; Mattheis, A. [Kernkraftwerke Gundremmingen Betriebsgesellschaft m.b.H. (Germany); Kerkhof, K.; Hippelein, K. [Stuttgart Univ. (Germany). Staatliche Materialpruefungsanstalt; Gurr-Beyer, C. [Buero fuer Baudynamik, Stuttgart (Germany); Hofstoetter, P. [Technischer Ueberwachungs-Verein Rheinland e.V., Koeln (Germany)

    1998-11-01

    Within the framework of periodic safety inspection of the Gundremmingen power station (RWE-Bayernwerk - KRB II), the load collectives used for the design of safety-relevant systems and components were checked for their consistency with latest updates of the design basis. It was found that there was no analytical information or study available describing a particular process and its effects, namely switch-off of the high-pressure feedwater pump of the emergency core cooling system. The paper reports the work performed for closing the gap, including preparatory analyses, accompanying measures such as vibration measurements during plant shut-down, as well as the preparation and performance of the in-situ test. The experimental results and the comparative evaluation of calculated and experimental data are presented. (orig./CB) [Deutsch] Im Rahmen der periodischen Sicherheitsueberpruefung des Kernkraftwerkes Gundremmingen (Kernkraftwerke RWE-Bayernwerk - KRB II) wurden u.a. die Lastkollektive, die zur Auslegung sicherheitstechnisch relevanter Systeme und Komponenten herangezogen wurden, auf Aktualitaet ueberprueft. Dabei zeigte sich, dass bislang fuer eine Betriebsweise - naemlich das Abschalten der Hochdruckeinspeisepumpe des nuklearen Not- und Nachkuehlsystems (TH-HD-Pumpe) - keine analytischen Untersuchungen vorliegen. Vorbetrachtungen fuer analytische Untersuchungen, begleitende Massnahmen wie Schwingungsmessungen waehrend des Anlagenstillstandes, sowie der Versuchsaufbau und die Versuchsdurchfuehrung des Anlagenversuches werden hier dargestellt. Die Ergebnisse und der Vergleich Rechnung-Messung zum Grossversuch werden in diesem Beitrag vorgestellt. (orig.)

  13. Satzstruktur und adverbiale Subordination. Eine Studie zum Deutschen und zum Italienischen

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    Hardarik Blühdorn

    2014-09-01

    Full Text Available This paper investigates the syntactic behaviour of adverbial clauses in contemporary German and Italian. It focuses on three main questions: (i How many degrees of syntactic integration of adverbial clauses are there to be distinguished by an adequate grammatical description of the two languages? (ii Which linear and hierarchical positions in the structure of the matrix sentence can be occupied by adverbial clauses? (iii Which is the empirical distribution of adverbial clauses introduced by the conjunctions als, während, wenn, obwohl and weil in German, as well as quando, mentre, se, sebbene and perché in Italian? Responding to question (i, a distinction is drawn between strongly integrated, weakly integrated and syntactically disintegrated adverbial clauses. There are further degrees on the gradient of syntactic integration, which are not examined in this paper. Responding to question (ii, eight classes of structural positions in the matrix sentence are identified that can be occupied by adverbial clauses. Five of them are positions of syntactic integration, three are positions of disintegration. Responding to question (iii, the distribution of the ten classes of adverbial clauses is described on the basis of a corpus of internet data. Strongly integrated, weakly integrated and disintegrated adverbial clauses show clearly different distributions within the structure of the matrix sentence. Also the semantic classes of adverbial clauses (temporal, adversative, conditional, concessive, causal are distributed differently.

  14. Methylation of NR3C1 is related to maternal PTSD, parenting stress and maternal medial prefrontal cortical activity in response to child separation among mothers with histories of violence exposure

    Science.gov (United States)

    Schechter, Daniel S.; Moser, Dominik A.; Paoloni-Giacobino, Ariane; Stenz, Ludwig; Gex-Fabry, Marianne; Aue, Tatjana; Adouan, Wafae; Cordero, María I.; Suardi, Francesca; Manini, Aurelia; Sancho Rossignol, Ana; Merminod, Gaëlle; Ansermet, Francois; Dayer, Alexandre G.; Rusconi Serpa, Sandra

    2015-01-01

    Prior research has shown that mothers with Interpersonal violence-related posttraumatic stress disorder (IPV-PTSD) report greater difficulty in parenting their toddlers. Relative to their frequent early exposure to violence and maltreatment, these mothers display dysregulation of their hypothalamic pituitary adrenal axis (HPA-axis), characterized by hypocortisolism. Considering methylation of the promoter region of the glucocorticoid receptor gene NR3C1 as a marker for HPA-axis functioning, with less methylation likely being associated with less circulating cortisol, the present study tested the hypothesis that the degree of methylation of this gene would be negatively correlated with maternal IPV-PTSD severity and parenting stress, and positively correlated with medial prefrontal cortical (mPFC) activity in response to video-stimuli of stressful versus non-stressful mother–child interactions. Following a mental health assessment, 45 mothers and their children (ages 12–42 months) participated in a behavioral protocol involving free-play and laboratory stressors such as mother–child separation. Maternal DNA was extracted from saliva. Interactive behavior was rated on the CARE-Index. During subsequent fMRI scanning, mothers were shown films of free-play and separation drawn from this protocol. Maternal PTSD severity and parenting stress were negatively correlated with the mean percentage of methylation of NR3C1. Maternal mPFC activity in response to video-stimuli of mother–child separation versus play correlated positively to NR3C1 methylation, and negatively to maternal IPV-PTSD and parenting stress. Among interactive behavior variables, child cooperativeness in play was positively correlated with NR3C1 methylation. Thus, the present study is the first published report to our knowledge, suggesting convergence of behavioral, epigenetic, and neuroimaging data that form a psychobiological signature of parenting-risk in the context of early life stress and PTSD

  15. Methylation of NR3C1 is related to maternal PTSD, parenting stress and maternal medial prefrontal cortical activity in response to child separation among mothers with histories of violence exposure

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    Daniel Scott Schechter

    2015-05-01

    Full Text Available Prior research has shown that mothers with Interpersonal Violence-related Posttraumatic Stress Disorder (IPV-PTSD report greater difficulty in parenting their toddlers. Relative to their frequent early exposure to violence and maltreatment, these mothers display dysregulation of their hypothalamic pituitary adrenal axis (HPA-axis, characterized by hypocortisolism. Considering methylation of the promoter region of the glucocorticoid receptor gene NR3C1 as a marker for HPA-axis functioning, with less methylation likely being associated with less circulating cortisol, the present study tested the hypothesis that the degree of methylation of this gene would be negatively correlated with maternal IPV-PTSD severity and parenting stress, and positively correlated with medial prefrontal cortical (mPFC activity in response to video-stimuli of stressful versus non-stressful mother-child interactions. Following a mental health assessment, 45 mothers and their children (ages 12-42 months participated in a behavioral protocol involving free-play and laboratory stressors such as mother-child separation. Maternal DNA was extracted from saliva. Interactive behavior was rated on the CARE-Index. During subsequent fMRI scanning, mothers were shown films of free-play and separation drawn from this protocol. Maternal PTSD severity and parenting stress were negatively correlated with the mean percentage of methylation of NR3C1. Maternal mPFC activity in response to video-stimuli of mother-child separation versus play correlated positively to NR3C1 methylation, and negatively to maternal IPV-PTSD and parenting stress. Among interactive behavior variables, child cooperativeness in play was positively correlated with NR3C1 methylation. Thus, the present study is the first published report to our knowledge, suggesting convergence of behavioral, epigenetic, and neuroimaging data that form a psychobiological signature of parenting-risk in the context of early life stress

  16. Nanocomposites of NR/SBR Blend Prepared by Latex Casting Method: Effects of Nano-TiO2 and Polystyrene-Encapsulated Nano-TiO2 on the Cure Characteristics, Physical Properties, and Morphology

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    Anyaporn Boonmahitthisud

    2017-01-01

    Full Text Available Nanocomposites of 80/20 (w/w natural rubber (NR/styrene butadiene rubber (SBR blend with four loadings of either nanosized titanium dioxide (nTiO2 or polystyrene-encapsulated nTiO2 (PS-nTiO2, ranging from 3 to 9 parts by weight per hundred of rubber (phr, were prepared by latex casting method. The PS-nTiO2 synthesized via in situ differential microemulsion polymerization displayed a core-shell morphology (nTiO2 core and PS shell with an average diameter of 42 nm. The cure characteristics (scorch time, cure time, and cure rate index, mechanical properties (tensile properties, tear strength, and hardness, thermal stability, glass transition temperature, and morphology of the prepared nanocomposites were quantified and compared. The results showed that the cure characteristics of all the nanocomposites were not significantly changed compared to those of the neat NR/SBR blend. The inclusion of an appropriate amount of either nTiO2 or PS-nTiO2 into the NR/SBR blend apparently improved the tensile strength, modulus at 300% strain, tear strength, hardness, and thermal stability but deteriorated the elongation at break of the nanocomposites. Based on differential scanning calorimetry, the glass transition temperature of all the nanocomposites was similar to that of the neat NR/SBR blend. Moreover, the morphology of the PS-nTiO2-filled rubber nanocomposites fractured surface analyzed by scanning electron microscopy showed an improvement in the interfacial adhesion between the rubber phase and the nanoparticles.

  17. Effect of liquid epoxidized natural rubber (LENR) on mechanical properties and morphology of natural rubber/high density polyethylene/mengkuang fiber (NR/HDPE/MK) bio-composite

    Science.gov (United States)

    Piah, Mohd Razi Mat; Baharum, Azizah

    2016-11-01

    The use of mengkuang fiber (MK) fibers in NR/HDPE (40/60) blend was studied via surface modification of fiber. The MK fiber was pre-washed with 5%wt/v sodium hydroxide solution prior to treatment with liquid epoxidized natural rubber (LENR). The concentration of LENR were varied from 5%-20%wt in toluene. The effects of LENR concentrations were studied in terms of mechanical properties and morphology formed. Melt-blending was performed using an internal mixer (Haake Rheomix 600). The processing parameters identified were 135°C temperature, 45 rpm rotor speed, 12 minutes processing time and at 20%wt MK fiber loading. The optimum LENR treatment concentration was obtained at 5%wt with tensile strength, tensile modulus, and impact strength of 10.3 MPa, 414.2 MPa and 14.4 kJ/m2 respectively. The tensile modulus of LENR-treated MK fiber filled NR/HDPE bio-composite has shown enhancement up to 16.7% higher than untreated MK fiber. The tensile and impact strength were decreased with increasing LENR concentration due to the broken of MK fibers to smaller particles and adhered to each other. FESEM micrographs confirmed the formation of fiber-fiber agglomeration in NR/HDPE blends. The optical microscope analysis shows MK fibers is shorter than original fiber lengths after NaOH-LENR surface modification. The internal bonding forces of MK fiber seems to be weaker than external force exerted on it, therefore, the MK fiber has broken to smaller particles and reduced the mechanical properties of NR/HDPE/MK(20%) bio-composite.

  18. PROTECŢIA PENALĂ A PATRIMONIULUI CULTURAL DIN PERSPECTIVA AMENDAMENTELOR OPERATE PRIN LEGEA NR.75/2016

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    Sergiu BRÎNZA

    2016-11-01

    Full Text Available În cadrul studiului sunt supuse interpretării prevederile normative introduse în Codul penal prin Legea nr.75/2016.În contextul interpretării alin.(21 art.186, alin.(21 art.187, alin.(21 art.188, alin.(21 art.190 şi alin.(22 art.191 CP RM,este stabilit conţinutul noţiunilor „bunuri de patrimoniu cultural”, „situri arheologice” şi „zone cu potenţial arheologic”.Se argumentează că infracţiunile prevăzute la art.1991 CP RM pot fi comise cu intenţie sau din imprudenţă. În vedereainterpretării dispoziţiilor art.1992 CP RM, este identificat semantismul noţiunilor „săpături neautorizate”, „comori”,„lucrări de construcţie”, „alte activităţi de intervenţie asupra solului” şi „certificat de descărcare de sarcină arheologică”.De asemenea, este stabilit criteriul de distincţie între tăinuirea sau păstrarea ilegală a bunurilor arheologice mobile (însensul art.1993 CP RM şi sustragerea aceloraşi bunuri (în sensul alin.(21 art.186, alin.(21 art.187, alin.(21 art.188,alin.(21 art.190 şi alin.(22 art.191 CP RM. Se demonstrează că administratorul terenului, în care s-a făcut descoperireaarheologică întâmplătoare, şi/sau reprezentantul responsabil al autorităţii administraţiei publice sunt cei care omit să anunţela timp autorităţile publice cu privire la descoperirea întâmplătoare a bunurilor arheologice mobile (inclusiv a comorilor.Nu în ultimul rând, se relevă că, în toate cazurile, monumentele de for public posedă calitatea de monumente care fac partedin patrimoniul cultural în sensul lit.a alin.(3 art.1 al Legii privind ocrotirea monumentelor. Utilizând în art.222 CP RMnoţiunea „monument de for public”, legiuitorul a luat implicit în considerare faptul că un asemenea monument este un bunaparţinând patrimoniului cultural naţional mobil. Cât priveşte monumentul funerar, şi acesta poate cumula calitatea demonument care face parte din patrimoniul

  19. Upregulation of the Nr2f1-A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of Waardenburg syndrome type 4.

    Science.gov (United States)

    Bergeron, Karl-F; Nguyen, Chloé M A; Cardinal, Tatiana; Charrier, Baptiste; Silversides, David W; Pilon, Nicolas

    2016-11-01

    Waardenburg syndrome is a neurocristopathy characterized by a combination of skin and hair depigmentation, and inner ear defects. In the type 4 form, these defects show comorbidity with Hirschsprung disease, a disorder marked by an absence of neural ganglia in the distal colon, triggering functional intestinal obstruction. Here, we report that the Spot mouse line - obtained through an insertional mutagenesis screen for genes involved in neural crest cell (NCC) development - is a model for Waardenburg syndrome type 4. We found that the Spot insertional mutation causes overexpression of an overlapping gene pair composed of the transcription-factor-encoding Nr2f1 and the antisense long non-coding RNA A830082K12Rik in NCCs through a mechanism involving relief of repression of these genes. Consistent with the previously described role of Nr2f1 in promoting gliogenesis in the central nervous system, we further found that NCC-derived progenitors of the enteric nervous system fail to fully colonize Spot embryonic guts owing to their premature differentiation in glial cells. Taken together, our data thus identify silencer elements of the Nr2f1-A830082K12Rik gene pair as new candidate loci for Waardenburg syndrome type 4. © 2016. Published by The Company of Biologists Ltd.

  20. Upregulation of the Nr2f1-A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of Waardenburg syndrome type 4

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    Karl-F. Bergeron

    2016-11-01

    Full Text Available Waardenburg syndrome is a neurocristopathy characterized by a combination of skin and hair depigmentation, and inner ear defects. In the type 4 form, these defects show comorbidity with Hirschsprung disease, a disorder marked by an absence of neural ganglia in the distal colon, triggering functional intestinal obstruction. Here, we report that the Spot mouse line – obtained through an insertional mutagenesis screen for genes involved in neural crest cell (NCC development – is a model for Waardenburg syndrome type 4. We found that the Spot insertional mutation causes overexpression of an overlapping gene pair composed of the transcription-factor-encoding Nr2f1 and the antisense long non-coding RNA A830082K12Rik in NCCs through a mechanism involving relief of repression of these genes. Consistent with the previously described role of Nr2f1 in promoting gliogenesis in the central nervous system, we further found that NCC-derived progenitors of the enteric nervous system fail to fully colonize Spot embryonic guts owing to their premature differentiation in glial cells. Taken together, our data thus identify silencer elements of the Nr2f1-A830082K12Rik gene pair as new candidate loci for Waardenburg syndrome type 4.

  1. Uranium metalla-allenes with carbene imido R_2C=U"I"V=NR' units (R=Ph_2PNSiMe_3; R'=CPh_3): alkali-metal-mediated push-pull effects with an amido auxiliary

    International Nuclear Information System (INIS)

    Lu, Erli; Tuna, Floriana; Kaltsoyannis, Nikolas; Liddle, Stephen T.; Lewis, William

    2016-01-01

    We report uranium(IV)-carbene-imido-amide metalla-allene complexes [U(BIPM"T"M"S)(NCPh_3)(NHCPh_3)(M)] (BIPM"T"M"S=C(PPh_2NSiMe_3)_2; M=Li or K) that can be described as R_2C=U=NR' push-pull metalla-allene units, as organometallic counterparts of the well-known push-pull organic allenes. The solid-state structures reveal that the R_2C=U=NR' units adopt highly unusual cis-arrangements, which are also reproduced by gas-phase theoretical studies conducted without the alkali metals to remove their potential structure-directing roles. Computational studies confirm the double-bond nature of the U=NR' and U=CR_2 interactions, the latter increasingly attenuated by potassium then lithium when compared to the hypothetical alkali-metal-free anion. Combined experimental and theoretical data show that the push-pull effect induced by the alkali metal cations and amide auxiliary gives a fundamental and tunable structural influence over the C=U"I"V=N units. (copyright 2016 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  2. O efeito de EPDM modificado com grupos mercapto ou tioacetato na cinética de vulcanização de misturas NR/EPDM The effect of mercapto- and thioacetate-modified EPDM on the vulcanization kinetics of NR/EPDM blends

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    Alex S. Sirqueira

    2006-12-01

    Full Text Available A cinética de vulcanização de misturas envolvendo borracha natural (NR e copolímero de etileno-propileno-dieno (EPDM na proporção 70:30 % em peso foi estudada em função da compatibilização. Para esse estudo foram utilizados 2,5 phr de EPDM funcionalizado com grupos tioacetato (EPDMTA ou mercaptana (EPDMSH. O estudo foi realizado em reômetro de disco oscilatório (curômetro. Ambos os copolímeros funcionalizados atuaram como aceleradores do processo de vulcanização das misturas NR/EPDM (70:30 na presença de enxofre e CBS, sendo EPDMSH mais efetivo do que EPDMTA pelo modelo de Chough e Chang. Entretanto, ao utilizar o modelo de Coran, observou-se que EPDMTA e EP-DMSH atuam em distintas etapas da vulcanização: EPDMSH atua preferencialmente no início do processo de vulcanização enquanto que EPDMTA aumenta a velocidade da formação das reticulações propriamente ditas. Além disso, EPDMTA diminuiu consideravelmente a tendência à degradação de reticulações, indicada pela menor taxa de reversão.Vulcanization kinetics for natural rubber (NR/ ethylene-propylene-diene rubber (EPDM blends was studied as a function of the compatibilization, by using an oscillatory disk rheometer. For this study, 2.5 phr of thioacetate- (EPDMTA or mercapto-modified EPDM (EPDMSH were employed in blends vulcanized with sulfur/CBS curing system. Both functionalized copolymers increased the curing velocity of the blends, but the higher overall rate was achieved with EPDMSH-modified systems, using the Chough and Chang model. However, when using the Coran model, we observed that they act in distinct vulcanization steps: EPDMSH acts preferentially at the beginning of the curing process whereas EPDMTA increases the rate of the crosslink formation. In addition, EPDMTA decreased the tendency for crosslink decomposition, as indicated by the lower value of reversion degree.

  3. Blockade of NMDA receptor subtype NR2B prevents seizures but not apoptosis of dentate gyrus neurons in bacterial meningitis in infant rats

    Science.gov (United States)

    Kolarova, Anna; Ringer, Ralph; Täuber, Martin G; Leib, Stephen L

    2003-01-01

    Background Excitotoxic neuronal injury by action of the glutamate receptors of the N-methyl-d-aspartate (NMDA) subtype have been implicated in the pathogenesis of brain damage as a consequence of bacterial meningitis. The most potent and selective blocker of NMDA receptors containing the NR2B subunit is (R,S)-alpha-(4-hydroxyphenyl)-beta-methyl-4-(phenylmethyl)-1-piperid inepropanol (RO 25-6981). Here we evaluated the effect of RO 25-6981 on hippocampal neuronal apoptosis in an infant rat model of meningitis due to Streptococcus pneumoniae. Animals were randomized for treatment with RO 25-6981 at a dosage of either 0.375 mg (15 mg/kg; n = 28) or 3.75 mg (150 mg/kg; n = 15) every 3 h or an equal volume of sterile saline (250 μl; n = 40) starting at 12 h after infection. Eighteen hours after infection, animals were assessed clinically and seizures were observed for a period of 2 h. At 24 h after infection animals were sacrificed and brains were examined for apoptotic injury to the dentate granule cell layer of the hippocampus. Results Treatment with RO 25-6981 had no effect on clinical scores, but the incidence of seizures was reduced (P < 0.05 for all RO 25-6981 treated animals combined). The extent of apoptosis was not affected by low or high doses of RO 25-6981. Number of apoptotic cells (median [range]) was 12.76 [3.16–25.3] in animals treated with low dose RO 25-6981 (control animals 13.8 [2.60–31.8]; (P = NS) and 9.8 [1.7–27.3] (controls: 10.5 [2.4–21.75]) in animals treated with high dose RO 25-6981 (P = NS). Conclusions Treatment with a highly selective blocker of NMDA receptors containing the NR2B subunit failed to protect hippocampal neurons from injury in this model of pneumococcal meningitis, while it had some beneficial effect on the incidence of seizures. PMID:13129439

  4. Geochemistry and Geochronology of Ngorongoro Crater, Tanzania: Implication for Magma Evolution, Duration of Volcanic Activity and Age of the Ngorongoro N-R Geomagnetic Polarity Transition

    Science.gov (United States)

    Mollel, G. F.; Swisher, C. C.; Feigenson, M. D.; Carr, M. J.

    2005-05-01

    40Ar/39Ar dates on volcanic rocks from the Ngorongoro Crater (NC) in northern Tanzania indicate that NC activity was very short in duration lasting approximately 120 ka. Laser incremental heating experiments on lava from the bottom and top of the NC crater-wall section gave ages of 2.08 +/- 0.04 and 1.96 +/- 0.02 Ma respectively. Lavas from the same section show a change in magnetic polarity from normal (N) at the lower part to reverse (R) polarity at the upper part (Grommé et al. 1970). The new ages are about 400 ka younger than previously estimated by K-Ar technique. These new ages suggest correlation of the NC N-R polarity transition to the 2.1 Ma (N-R) Reunion-Matuyama boundary (Cande and Kent, 1995), instead of the Gauss-Matuyama boundary as proposed by Grommé et al. (1970). 87Sr/86Sr measurements on lavas from the NC section vary widely from 0.70801 in the trachydacite at the base to 0.70405 in the basaltic lava near the top. The lower part of the section is more radiogenic varying from 0.70592 to 0.70801 whereas the upper part is constrained to 0.70405 to 0.70450. The more radiogenic lower part is likely to have interacted with crustal rocks. Two possible contaminants are the Tanzanian Archean Craton to the west and the late Proterozoic Mozambican belt in the east. The crater-wall section is composed of trachydacite at the bottom that becomes trachyandesite in mid-section. The top section is mainly basaltic. Major and trace elements show an inverted geochemical signature that is typical of stratified magma chambers characterized by a silicic top and basaltic bottom. Olivine basalt at the upper part of the section has the highest Mg# (56.60) and in general the upper section is more mafic than the lower section as inferred from Mg#. The upper part of the section is high in TiO2, MgO, FeOT, and CaO wt% whereas SiO2 and K2O wt% are higher in lower part of the section. No significant variations are observed in N2O, Al2O3, P2O5 and MnO wt% up-section. Highly

  5. Common polymorphisms within the NR4A3 locus, encoding the orphan nuclear receptor Nor-1, are associated with enhanced β-cell function in non-diabetic subjects

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    Kuusisto Johanna

    2009-08-01

    Full Text Available Abstract Background Neuron-derived orphan receptor (Nor 1, nuclear receptor (Nur 77, and nuclear receptor-related protein (Nurr 1 constitute the NR4A family of orphan nuclear receptors which were recently found to modulate hepatic glucose production, insulin signalling in adipocytes, and oxidative metabolism in skeletal muscle. In this study, we assessed whether common genetic variation within the NR4A3 locus, encoding Nor-1, contributes to the development of prediabetic phenotypes, such as glucose intolerance, insulin resistance, or β-cell dysfunction. Methods We genotyped 1495 non-diabetic subjects from Southern Germany for the five tagging single nucleotide polymorphisms (SNPs rs7047636, rs1526267, rs2416879, rs12686676, and rs10819699 (minor allele frequencies ≥ 0.05 covering 100% of genetic variation within the NR4A3 locus (with D' = 1.0, r2 ≥ 0.9 and assessed their association with metabolic data derived from the fasting state, an oral glucose tolerance test (OGTT, and a hyperinsulinemic-euglycemic clamp (subgroup, N = 506. SNPs that revealed consistent associations with prediabetic phenotypes were subsequently genotyped in a second cohort (METSIM Study; Finland; N = 5265 for replication. Results All five SNPs were in Hardy-Weinberg equilibrium (p ≥ 0.7, all. The minor alleles of three SNPs, i.e., rs1526267, rs12686676, and rs10819699, consistently tended to associate with higher insulin release as derived from plasma insulin at 30 min(OGTT, AUCC-peptide-to-AUCGluc ratio and the AUCIns30-to-AUCGluc30 ratio with rs12686676 reaching the level of significance (p ≤ 0.03, all; additive model. The association of the SNP rs12686676 with insulin secretion was replicated in the METSIM cohort (p ≤ 0.03, additive model. There was no consistent association with glucose tolerance or insulin resistance in both study cohorts. Conclusion We conclude that common genetic variation within the NR4A3 locus determines insulin secretion. Thus, NR4A3

  6. Deletion of the NR4A nuclear receptor NOR1 in hematopoietic stem cells reduces inflammation but not abdominal aortic aneurysm formation.

    Science.gov (United States)

    Qing, Hua; Jones, Karrie L; Heywood, Elizabeth B; Lu, Hong; Daugherty, Alan; Bruemmer, Dennis

    2017-10-18

    The NR4A3 orphan nuclear hormone receptor, NOR1, functions as a constitutively active transcription factor to regulate inflammation, proliferation, and cell survival during pathological vascular remodeling. Inflammatory processes represent key mechanisms leading to abdominal aortic aneurysm (AAA) formation. However, a role of NOR1 in AAA formation has not been investigated previously. Inflammatory gene expression was analyzed in bone marrow-derived macrophages isolated from NOR1-deficient mice. Low-density lipoprotein receptor-deficient (LDLr -/- ) mice were irradiated and reconstituted with hematopoietic stem cells obtained from NOR1-/- or wild-type littermate mice. Animals were infused with angiotensin II and fed a diet enriched in saturated fat to induce AAA formation. Quantification of AAA formation was performed by ultrasound and ex vivo measurements. Among 184 inflammatory genes that were analyzed, 36 genes were differentially regulated in LPS-treated NOR1-deficient macrophages. Albeit this difference in gene regulation, NOR1-deficiency in hematopoietic stem cells did not affect development of AAA formation in bone marrow-derived stem cell transplanted LDLr-deficient mice. NOR1 deletion induced differential inflammatory gene transcription in macrophages but did not influence AAA formation in mice.

  7. The Niwot Ridge Subalpine Forest US-NR1 AmeriFlux site - Part 1: Data acquisition and site record-keeping

    Science.gov (United States)

    Burns, Sean P.; Maclean, Gordon D.; Blanken, Peter D.; Oncley, Steven P.; Semmer, Steven R.; Monson, Russell K.

    2016-09-01

    The Niwot Ridge Subalpine Forest AmeriFlux site (US-NR1) has been measuring eddy-covariance ecosystem fluxes of carbon dioxide, heat, and water vapor since 1 November 1998. Throughout this 17-year period there have been changes to the instrumentation and improvements to the data acquisition system. Here, in Part 1 of this three-part series of papers, we describe the hardware and software used for data-collection and metadata documentation. We made changes to the data acquisition system that aimed to reduce the system complexity, increase redundancy, and be as independent as possible from any network outages. Changes to facilitate these improvements were (1) switching to a PC/104-based computer running the National Center for Atmospheric Research (NCAR) In-Situ Data Acquisition Software (NIDAS) that saves the high-frequency data locally and over the network, and (2) time-tagging individual 10 Hz serial data samples using network time protocol (NTP) coupled to a GPS-based clock, providing a network-independent, accurate time base. Since making these improvements almost 2 years ago, the successful capture of high-rate data has been better than 99.98 %. We also provide philosophical concepts that shaped our design of the data system and are applicable to many different types of environmental data collection.

  8. The effect of gamma radiation on the ageing of sulfur cured nr/csm and nbr/csm rubber blends reinforced by carbon black

    Directory of Open Access Journals (Sweden)

    Gordana Marković

    2009-10-01

    Full Text Available In this work the effect of the γ-radiation dose on ageing of carbon black reinforced elastomeric materials was studied. The compounds based on natural rubber/chlorosulfonated rubber blend (NR/CSM and butadiene acrylonitrile rubber/chlorosulfonated rubber blend (NBR/CSM (50:50, w/w with different loadings (0, 20, 40, 50, 60, 80 and 100 phr of the filler with the average particle size of 40 nm were cured by sulfur. The obtained elastomeric composites were subjected to radiation doses (100, 200, 300 and 400 kGy in the presence of oxygen. The changes of material mechanical properties were estimated after radiation accelerated ageing. By using Fourier transform infrared measurements (ATR-FTIR it was assessed that after exposure to doses of 100 kGy alcohols, ethers, lactones, anhydrides, esters and carboxylic acids are formed in materials. The formation of shorter polyene sequences and aromatic rings in aged samples are assumed on the basis of the obtained spectra.

  9. Caloric restriction increases learning consolidation and facilitates synaptic plasticity through mechanisms dependent on NR2B subunits of the NMDA receptor.

    Science.gov (United States)

    Fontán-Lozano, Angela; Sáez-Cassanelli, José Luis; Inda, Mari Carmen; de los Santos-Arteaga, Mercedes; Sierra-Domínguez, Sergio Antonio; López-Lluch, Guillermo; Delgado-García, José María; Carrión, Angel Manuel

    2007-09-19

    One of the main focal points of aging research is the search for treatments that will prevent or ameliorate the learning and memory deficiencies associated with aging. Here we have examined the effects of maintaining mature mice on a long-term intermittent fasting diet (L-IFD). We found that L-IFD enhances learning and consolidation processes. We also assessed the long-term changes in synaptic efficiency in these animals. L-IFD mice showed an increase in low-theta-band oscillations, paired-pulse facilitation, and facilitation of long-term synaptic plasticity in the hippocampus with respect to mice fed ad libitum. In addition, we found an increase in the expression of the NMDA receptor subunit NR2B in some brain areas of L-IFD mice. Specific antagonism of this subunit in the hippocampus reversed the beneficial effects of L-IFD. These data provide a molecular and cellular mechanism by which L-IFD may enhance cognition, ameliorating some aging-associated cognitive deficits.

  10. EQ3NR, a computer program for geochemical aqueous speciation-solubility calculations: Theoretical manual, user`s guide, and related documentation (Version 7.0); Part 3

    Energy Technology Data Exchange (ETDEWEB)

    Wolery, T.J.

    1992-09-14

    EQ3NR is an aqueous solution speciation-solubility modeling code. It is part of the EQ3/6 software package for geochemical modeling. It computes the thermodynamic state of an aqueous solution by determining the distribution of chemical species, including simple ions, ion pairs, and complexes, using standard state thermodynamic data and various equations which describe the thermodynamic activity coefficients of these species. The input to the code describes the aqueous solution in terms of analytical data, including total (analytical) concentrations of dissolved components and such other parameters as the pH, pHCl, Eh, pe, and oxygen fugacity. The input may also include a desired electrical balancing adjustment and various constraints which impose equilibrium with special pure minerals, solid solution end-member components (of specified mole fractions), and gases (of specified fugacities). The code evaluates the degree of disequilibrium in terms of the saturation index (SI = 1og Q/K) and the thermodynamic affinity (A = {minus}2.303 RT log Q/K) for various reactions, such as mineral dissolution or oxidation-reduction in the aqueous solution itself. Individual values of Eh, pe, oxygen fugacity, and Ah (redox affinity) are computed for aqueous redox couples. Equilibrium fugacities are computed for gas species. The code is highly flexible in dealing with various parameters as either model inputs or outputs. The user can specify modification or substitution of equilibrium constants at run time by using options on the input file.

  11. NR sulphur vulcanization: Interaction study between TBBS and DPG by means of a combined experimental rheometer and meta-model best fitting strategy

    Energy Technology Data Exchange (ETDEWEB)

    Milani, G., E-mail: gabriele.milani@polimi.it [Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan (Italy); Hanel, T.; Donetti, R. [Pirelli Tyre, Via Alberto e Piero Pirelli 25, 20126 Milan (Italy); Milani, F. [Chem. Co, Via J.F. Kennedy 2, 45030 Occhiobello (Italy)

    2016-06-08

    The paper is aimed at studying the possible interaction between two different accelerators (DPG and TBBS) in the chemical kinetic of Natural Rubber (NR) vulcanized with sulphur. The same blend with several DPG and TBBS concentrations is deeply analyzed from an experimental point of view, varying the curing temperature in the range 150-180°C and obtaining rheometer curves with a step of 10°C. In order to study any possible interaction between the two accelerators –and eventually evaluating its engineering relevance-rheometer data are normalized by means of the well known Sun and Isayev normalization approach and two output parameters are assumed as meaningful to have an insight into the possible interaction, namely time at maximum torque and reversion percentage. Two different numerical meta-models, which belong to the family of the so-called response surfaces RS are compared. The first is linear against TBBS and DPG and therefore well reproduces no interaction between the accelerators, whereas the latter is a non-linear RS with bilinear term. Both RS are deduced from standard best fitting of experimental data available. It is found that, generally, there is a sort of interaction between TBBS and DPG, but that the error introduced making use of a linear model (no interaction) is generally lower than 10%, i.e. fully acceptable from an engineering standpoint.

  12. EQ3NR, a computer program for geochemical aqueous speciation-solubility calculations: Theoretical manual, user's guide, and related documentation (Version 7.0)

    International Nuclear Information System (INIS)

    Wolery, T.J.

    1992-01-01

    EQ3NR is an aqueous solution speciation-solubility modeling code. It is part of the EQ3/6 software package for geochemical modeling. It computes the thermodynamic state of an aqueous solution by determining the distribution of chemical species, including simple ions, ion pairs, and complexes, using standard state thermodynamic data and various equations which describe the thermodynamic activity coefficients of these species. The input to the code describes the aqueous solution in terms of analytical data, including total (analytical) concentrations of dissolved components and such other parameters as the pH, pHCl, Eh, pe, and oxygen fugacity. The input may also include a desired electrical balancing adjustment and various constraints which impose equilibrium with special pure minerals, solid solution end-member components (of specified mole fractions), and gases (of specified fugacities). The code evaluates the degree of disequilibrium in terms of the saturation index (SI = 1og Q/K) and the thermodynamic affinity (A = -2.303 RT log Q/K) for various reactions, such as mineral dissolution or oxidation-reduction in the aqueous solution itself. Individual values of Eh, pe, oxygen fugacity, and Ah (redox affinity) are computed for aqueous redox couples. Equilibrium fugacities are computed for gas species. The code is highly flexible in dealing with various parameters as either model inputs or outputs. The user can specify modification or substitution of equilibrium constants at run time by using options on the input file

  13. HR38, an ortholog of NR4A family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction.

    Science.gov (United States)

    Dong, Dujuan; Zhang, Yang; Smykal, Vlastimil; Ling, Lin; Raikhel, Alexander S

    2018-05-01

    The Aedes aegypti mosquito is the principal vector for many dangerous human viral diseases. Carbohydrate metabolism (CM) is essential for supplying the energy necessary for host seeking, blood digestion and rapid egg development of this vector insect. The steroid hormone 20-hydroxyecdysone (20E) and the ecdysone receptor (EcR) are important regulators of CM, coordinating it with female reproductive events. We report here that the NR4A nuclear receptor AHR38 plays a critical role in mediating these actions of 20E and EcR. AHR38 RNA interference (RNAi) depletion in female mosquitoes blocked the transcriptional activation of CM genes encoding phosphoglucomutase (PGM) and trehalose-6-phophate synthase (TPS); it caused an increase of glycogen accumulation and a decrease of the circulating sugar trehalose. This treatment also resulted in a dramatic reduction in fecundity. Considering that these phenotypes resulting from AHR38 RNAi depletion are similar to those of EcR RNAi, we investigated a possible connection between these transcription factors in CM regulation. EcR RNAi inhibits the AHR38 gene expression. Moreover, the 20E-induced EcR complex directly activates AHR38 by binding to the ecdysone response element (EcRE) in the upstream regulatory region of this gene. The present work has implicated AHR38 in the 20E-mediated control of CM and provided new insight into mechanisms of 20E regulation of metabolism during female mosquito reproduction. © 2018 Published by Elsevier Ltd.

  14. Interim Report: 100-NR-2 Apatite Treatability Test: Low Concentration Calcium Citrate-Phosphate Solution Injection for In Situ Strontium-90 Immobilization

    Energy Technology Data Exchange (ETDEWEB)

    Williams, Mark D.; Fritz, Brad G.; Mendoza, Donaldo P.; Rockhold, Mark L.; Thorne, Paul D.; Xie, YuLong; Bjornstad, Bruce N.; Mackley, Rob D.; Newcomer, Darrell R.; Szecsody, James E.; Vermeul, Vincent R.

    2008-07-11

    Following an evaluation of potential Sr-90 treatment technologies and their applicability under 100-NR-2 hydrogeologic conditions, U.S. Department of Energy, Fluor Hanford, Inc., Pacific Northwest National Laboratory, and the Washington Department of Ecology agreed that the long-term strategy for groundwater remediation at 100-N Area will include apatite sequestration as the primary treatment, followed by a secondary treatment if necessary (most likely phytoremediation). Since then, the agencies have worked together to agree on which apatite sequestration technology has the greatest chance of reducing Sr-90 flux to the river at a reasonable cost. In July 2005, aqueous injection, (i.e., the introduction of apatite-forming chemicals into the subsurface) was endorsed as the interim remedy and selected for field testing. Studies are in progress to assess the efficacy of in situ apatite formation by aqueous solution injection to address both the vadose zone and the shallow aquifer along the 300 ft of shoreline where Sr-90 concentrations are highest. This report describes the field testing of the shallow aquifer treatment.

  15. Germanene nanoribbon tunneling field effect transistor (GeNR-TFET) with a 10 nm channel length: analog performance, doping and temperature effects

    International Nuclear Information System (INIS)

    Bayani, Amir Hossein; Vali, Mehran; Dideban, Daryoosh; Moezi, Negin

    2016-01-01

    In this paper, a scheme of the germanene nanoribbon tunneling field effect transistor (GeNR-TFET) is proposed. The characteristics and analog performance of the device were theoretically investigated by exploiting the electrical properties of a germanene nanoribbon and applying the doping concentration in the source and drain regions at 300 K and 4 K temperatures. The device parameters were obtained using a non-equilibrium Green’s function (NEGF) method within the tight binding (TB) Hamiltonian. The TB Hamiltonian was extracted from the density functional theory (DFT) through the Wannier function. We find that by increasing the doping concentration the I on current increases which leads to an improvement of the I on /I off ratio to 10 5 . Moreover, decreasing the temperature from 300 K to 4 K causes the I off to become ten times smaller. We find that the device output characteristic displays a negative differential conductance with a good peak-to-valley ratio which is improved by increasing the doping concentration. The analog performance of the device is also investigated in the subthreshold regime of operation by varying the doping concentration. It is observed that by increasing the device doping concentration, the analog figures of merit can be improved. (paper)

  16. High-productivity laser deposition welding using powder and wire. Final report of the project 'Higher efficiency of thermal coating plants by a combination of energy sources'; Hochproduktives Laser-Auftragschweissen mit Pulver und Draht. Ergebnisbericht zum Verbundvorhaben ''Wirkungsgradsteigerung thermischer Beschichtungsanlagen durch Energiequellenkombination''. SPRAYNERGY

    Energy Technology Data Exchange (ETDEWEB)

    Nowotny, Steffen (ed.)

    2013-05-01

    Excellent precision, maximum mechanical strength and selective properties of the generated coatings and 3D structures caused a breakthrough of laser deposition welding in industrial production. However, the technology is limited by its low deposition rates in those cases where large-surface components are to be coated while laser-typical corrosion and wear resistance characteristics are to be achieved. Examples are, e.g. hydraulic cylinders for offshore applications, tools for petroleum or gas production and mining, or large-scale conveyors. In these cases, modern laser hybrid technologies open up new applications. By effectively combining the laser energy with other energy sources, e.g. inductive heating, autogenic flames or electric resistance, high deposition rates are achieved with excellent coating qualities, which makes the laser process competitive also in the case of large-surface coatings. (orig./AKB) [German] Exzellente Praezision, hoechste mechanische Festigkeit und massgeschneiderte Eigenschaften von Oberflaechenschichten wie auch generativ erzeugten 3D-Strukturen: diese Merkmale haben dem Auftragschweissen mit Laserstrahlen zum Durchbruch in der industriellen Produktion verholfen. Die geringen Auftragraten setzen der Technologie jedoch in jenen Faellen Grenzen, wo es um Flaechenbeschichtungen grosser Bauteile geht, deren Einsatz aber dennoch die lasertypischen Schichteigenschaften hinsichtlich Korrosions- oder Verschleissbestaendigkeit erfordert. Beispiele sind lange Hydraulikzylinder im Offshore-Bereich, Werkzeuge der Oel- und Gasfoerderung und des Bergbaus sowie grosse Foerderschnecken. Hier erweitern moderne Laser-Hybridtechnologien wirkungsvoll die Einsatzmoeglichkeiten. Durch effiziente Kombination der Laserenergie mit anderen Energiequellen in Form induktiver Erwaermung, autogener Flammen oder elektrischem Widerstand werden hohe Auftragraten bei gleichzeitigem Erhalt der besonderen Schichtqualitaet erreicht, die das Laserverfahren auch bei

  17. Experimental investigations for provision of a data basis for optimization of active surge control. Final report; Experimentelle Untersuchungen zur Bereitstellung einer Datenbasis fuer die Optimierung der aktiven Stabilitaetsverbesserung. Teilverbund-Projekt: Aktive Stabilitaetsverbesserung - Analyseverfahren der Arbeitsgemeinschaft AG Turbo II. Abschlussbericht zum Vorhaben 1.1.4

    Energy Technology Data Exchange (ETDEWEB)

    Seume, J.; Reissner, A.

    2001-07-01

    Presently axial compressors have to run with considerable distance to operation points of high load although usually the best efficiencies can be obtained there. In those areas there is high danger of surge which brings high damaging potential to the compressor. The prediction time to surge presently is very short (<<1s) and depends on the load and rotor rotation of the compressor. The goal of this project is the production of a data basis to investigate stall inception (which is the precursor of surge) and to develop and test better precursory warning algorithms. An extensive set of data as well in stable as in unstable operating points in different load areas was developed which now is ready for use in research, verification and for comparison of forewarning systems. (orig.) [German] Zur Zeit ist es notwendig, Axialverdichter mit deutlichem Abstand von den hoch belasteten Betriebspunkten zu betreiben, obwohl haeufig dort die besten Wirkungsgrade zu erzielen waeren. In diesen Bereichen kann es leicht zu sog. Pumpen kommen, wodurch der Verdichter einem extremen Schaedigungspotential ausgesetzt ist. Die Vorwarnzeiten fuer das Pumpen sind bisher noch sehr kurz. Sie liegen in einem Bereich von deutlich weniger als einer Sekunde (abhaengig von der Belastung und der Drehzahl des Verdichters). Ziel dieser Untersuchungen ist es, eine Datenbasis zu liefern, anhand derer die Entstehungsmechanismen untersucht und moeglichst fruehzeitige Vorwarnalgorithmen entwickelt bzw. erprobt werden koennen. Es wurde eine umfangreiche Datenbasis sowohl an verschiedenen stabilen als auch instabilen Betriebspunkten in unterschiedlichen Lastbereichen erstellt, die nun zur Entwicklung, zur Verifizierung und zum Vergleich von Fruehwarnsystemen zur Verfuegung steht. (orig.)

  18. How does the German executive judge the risk of a global climate change? An opinion survey on the risk management in environmental policy; Wie schaetzt die deutsche Exekutive die Gefahr eines globalen Klimawandels ein? Eine Meinungsumfrage zum Risikomanagement in der Umweltpolitik

    Energy Technology Data Exchange (ETDEWEB)

    Krueck, C. [VDI-Technologiezentrum Physikalische Technologien, Duesseldorf (Germany). Abt. Zukuenftige Technologien; Bray, D. [GKSS-Forschungszentrum Geesthacht GmbH (Germany). Inst. fuer Gewaesserphysik

    2000-07-01

    Global warming is a problem which is scientifically complex and risky for political institutions. Coping with this problem requires elaborate methods of information exchange and accommodation between scientific and political institutions in order to facilitate risk management and decisions under uncertainty. This paper explores the relationship between science and politics based on the results of a survey of representatives of the German executive branch (both on the Federal and the Laender level) who are in charged with regulating environmental, nature and climate protection affairs. Results indicate that the top-level administrative staff in Germany perceive climate change as an urgent problem but are undecided whether 'climate research has managed to remain a neutral, value-free science'. Climate change is perceived as an exceptionally complex problem for science which is still faced with numerous knowledge gaps. At present, the relation problem for science which is still faced with numerous knowledge gaps. At present, the relation between the executive branch and climate research is trust-based but also quite distanced, particularly, at the regional (Laender) level. (orig.) [German] Die Warnung vor einer globalen Erwaermung konfrontiert die Politik mit einem Problem, das wissenschaftlich komplex und politisch riskant ist. Die Bewaeltigung derartiger Probleme erfordert ein hohes Mass an Informationsaustausch und Verstaendigung zwischen politischen und wissenschaftlichen Institutionen, um Risikomanagement zu betreiben und Entscheidungen unter Unsicherheit zu treffen. Um ein genaueres Bild davon zu gewinnen, wie die Bedrohung des Klimawandels in der deutschen Politik wahrgenommen und handhabbar gemacht wird, wurde ein wichtiges Segment des deutschen politischen Systems unter die Lupe genommen: Fuehrende Vertreter der Exekutive (Verwaltung), die auf Bundes- und Laenderebene mit Fragen des Umwelt-Natur- und Klimaschutzes befasst sind, wurden zum Thema

  19. Neutron Diffraction Studies of a Class A beta-Lactamase Toho-1 E166A/R274N/R276N Triple Mutant

    International Nuclear Information System (INIS)

    Blakeley, Matthew P.; Chen, Yu; Afonine, Pavel

    2010-01-01

    beta-Lactam antibiotics have been used effectively over several decades against many types of bacterial infectious diseases. However, the most common cause of resistance to the beta-lactam antibiotics is the production of beta-lactamase enzymes that inactivate beta-lactams by rapidly hydrolyzing the amide group of the beta-lactam ring. Specifically, the class A extended-spectrum beta-lactamases (ESBLs) and inhibitor-resistant enzymes arose that were capable of hydrolyzing penicillins and the expanded-spectrum cephalosporins and monobactams in resistant bacteria, which lead to treatment problems in many clinical settings. A more complete understanding of the mechanism of catalysis of these ESBL enzymes will impact current antibiotic drug discovery efforts. Here, we describe the neutron structure of the class A, CTX-M-type ESBL Toho-1 E166A/R274N/R276N triple mutant in its apo form, which is the first reported neutron structure of a beta-lactamase enzyme. This neutron structure clearly reveals the active-site protonation states and hydrogen-bonding network of the apo Toho-1 ESBL prior to substrate binding and subsequent acylation. The protonation states of the active-site residues Ser70, Lys73, Ser130, and Lys234 in this neutron structure are consistent with the prediction of a proton transfer pathway from Lys73 to Ser130 that is likely dependent on the conformation of Lys73, which has been hypothesized to be coupled to the protonation state of Glu166 during the acylation reaction. Thus, this neutron structure is in agreement with a proposed mechanism for acylation that identifies Glu166 as the general base for catalysis.

  20. Phylogeography and molecular diversity analysis of Jatropha curcas L. and the dispersal route revealed by RAPD, AFLP and nrDNA-ITS analysis.

    Science.gov (United States)

    Sudheer Pamidimarri, D V N; Reddy, Muppala P

    2014-05-01

    Jatropha curcas L. (Euphorbiaceae) has acquired a great importance as a renewable source of energy with a number of environmental benefits. Very few attempts were made to understand the extent of genetic diversity and its distribution. This study was aimed to study the diversity and deduce the phylogeography of Jatropha curcas L. which is said to be the most primitive species of the genus Jatropha. Here we studied the intraspecific genetic diversity of the species distributed in different parts of the globe. The study also focused to understand the molecular diversity at reported probable center of origin (Mexico), and to reveal the dispersal route to other regions based on random amplified polymorphic DNA, amplified fragment length polymorphism and nrDNA-ITS sequences data. The overall genetic diversity of J. curcas found in the present study was narrow. The highest genetic diversity was observed in the germplasm collected from Mexico and supports the earlier hypothesis based on morphological data and natural distribution, it is the center for origin of the species. Least genetic diversity found in the Indian germplasm and clustering results revealed that the species was introduced simultaneously by two distinct germplasm and subsequently distributed in different parts of India. The present molecular data further revealed that J. curcas might have spread from the center of the origin to Cape Verde, than to Spain, Portuguese to other neighboring countries and simultaneously to Africa. The molecular evidence supports the Burkill et al. (A dictionary of the economic products of the Malay Peninsula, Governments of Malaysia and Singapore by the Ministry of Agriculture and Co-operatives. Kuala Lumpur, Malaysia, 1966) view of Portuguese might have introduced the species to India. The clustering pattern suggests that the distribution was interfered by human activity.

  1. Dopamine receptor D5 deficiency results in a selective reduction of hippocampal NMDA receptor subunit NR2B expression and impaired memory.

    Science.gov (United States)

    Moraga-Amaro, Rodrigo; González, Hugo; Ugalde, Valentina; Donoso-Ramos, Juan Pablo; Quintana-Donoso, Daisy; Lara, Marcelo; Morales, Bernardo; Rojas, Patricio; Pacheco, Rodrigo; Stehberg, Jimmy

    2016-04-01

    Pharmacological evidence associates type I dopamine receptors, including subtypes D1 and D5, with learning and memory. Analyses using genetic approaches have determined the relative contribution of dopamine receptor D1 (D1R) in cognitive tasks. However, the lack of drugs that can discriminate between D1R and D5R has made the pharmacological distinction between the two receptors difficult. Here, we aimed to determine the role of D5R in learning and memory. In this study we tested D5R knockout mice and wild-type littermates in a battery of behavioral tests, including memory, attention, locomotion, anxiety and motivational evaluations. Our results show that genetic deficiency of D5R significantly impairs performance in the Morris water maze paradigm, object location and object recognition memory, indicating a relevant role for D5R in spatial memory and recognition memory. Moreover, the lack of D5R resulted in decreased exploration and locomotion. In contrast, D5R deficiency had no impact on working memory, anxiety and depressive-like behavior, measured using the spontaneous alternation, open-field, tail suspension test, and forced swimming test. Electrophysiological analyses performed on hippocampal slices showed impairment in long-term-potentiation in mice lacking D5R. Further analyses at the molecular level showed that genetic deficiency of D5R results in a strong and selective reduction in the expression of the NMDA receptor subunit NR2B in the hippocampus. These findings demonstrate the relevant contribution of D5R in memory and suggest a functional interaction of D5R with hippocampal glutamatergic pathways. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Phylogeography and molecular diversity analysis of Jatropha curcas L. and the dispersal route revealed by RAPD, AFLP and nrDNA-ITS analysis

    KAUST Repository

    Sudheer Pamidimarri, D. V N

    2014-01-29

    Jatropha curcas L. (Euphorbiaceae) has acquired a great importance as a renewable source of energy with a number of environmental benefits. Very few attempts were made to understand the extent of genetic diversity and its distribution. This study was aimed to study the diversity and deduce the phylogeography of Jatropha curcas L. which is said to be the most primitive species of the genus Jatropha. Here we studied the intraspecific genetic diversity of the species distributed in different parts of the globe. The study also focused to understand the molecular diversity at reported probable center of origin (Mexico), and to reveal the dispersal route to other regions based on random amplified polymorphic DNA, amplified fragment length polymorphism and nrDNA-ITS sequences data. The overall genetic diversity of J. curcas found in the present study was narrow. The highest genetic diversity was observed in the germplasm collected from Mexico and supports the earlier hypothesis based on morphological data and natural distribution, it is the center for origin of the species. Least genetic diversity found in the Indian germplasm and clustering results revealed that the species was introduced simultaneously by two distinct germplasm and subsequently distributed in different parts of India. The present molecular data further revealed that J. curcas might have spread from the center of the origin to Cape Verde, than to Spain, Portuguese to other neighboring countries and simultaneously to Africa. The molecular evidence supports the Burkill et al. (A dictionary of the economic products of the Malay Peninsula, Governments of Malaysia and Singapore by the Ministry of Agriculture and Co-operatives. Kuala Lumpur, Malaysia, 1966) view of Portuguese might have introduced the species to India. The clustering pattern suggests that the distribution was interfered by human activity. © Springer Science+Business Media 2014.

  3. Electrophysiological evidence for the presence of NR2C subunits of N-methyl-D-aspartate receptors in rat neurons of the nucleus tractus solitarius

    Directory of Open Access Journals (Sweden)

    V. Baptista

    2005-01-01

    Full Text Available The nucleus tractus solitarius (NTS plays an important role in the control of autonomic reflex functions. Glutamate, acting on N-methyl-D-aspartate (NMDA and non-NMDA ionotropic receptors, is the major neurotransmitter in this nucleus, and the relative contribution of each receptor to signal transmission is unclear. We have examined NMDA excitatory postsynaptic currents (NMDA-EPSCs in the subpostremal NTS using the whole cell patch clamp technique on a transverse brainstem slice preparation. The NMDA-EPSCs were evoked by stimulation of the solitary tract over a range of membrane potentials. The NMDA-EPSCs, isolated pharmacologically, presented the characteristic outward rectification and were completely blocked by 50 µM DL-2-amino-5-phosphonopentanoic acid. The I-V relationship of the NMDA response shows that current, with a mean (± SEM amplitude of -41.2 ± 5.5 pA, is present even at a holding potential of -60 mV, suggesting that the NMDA receptors are weakly blocked by extracellular Mg2+ at near resting membrane potentials. This weak block can also be inferred from the value of 0.67 ± 0.17 for parameter delta obtained from a fit of the Woodhull equation to the I-V relationship. The maximal inward current measured on the I-V relationship was at -38.7 ± 4.2 mV. The decay phase of the NMDA currents was fitted with one exponential function with a decay time constant of 239 ± 51 and 418 ± 80 ms at a holding potential of -60 and +50 mV, respectively, which became slower with depolarization (e-fold per 145 mV. The biophysical properties of the NMDA receptors observed in the present study suggest that these receptors in the NTS contain NR2C subunits and may contribute to the synaptic signal integration.

  4. Transcriptional Regulation of S Phase Kinase-associated Protein 2 by NR4A Orphan Nuclear Receptor NOR1 in Vascular Smooth Muscle Cells*

    Science.gov (United States)

    Gizard, Florence; Zhao, Yue; Findeisen, Hannes M.; Qing, Hua; Cohn, Dianne; Heywood, Elizabeth B.; Jones, Karrie L.; Nomiyama, Takashi; Bruemmer, Dennis

    2011-01-01

    Members of the NR4A subgroup of the nuclear hormone receptor superfamily have emerged as key transcriptional regulators of proliferation and inflammation. NOR1 constitutes a ligand-independent transcription factor of this subgroup and induces cell proliferation; however, the transcriptional mechanisms underlying this mitogenic role remain to be defined. Here, we demonstrate that the F-box protein SKP2 (S phase kinase-associated protein 2), the substrate-specific receptor of the ubiquitin ligase responsible for the degradation of p27KIP1 through the proteasome pathway, constitutes a direct transcriptional target for NOR1. Mitogen-induced Skp2 expression is silenced in vascular smooth muscle cells (VSMC) isolated from Nor1-deficient mice or transfected with Nor1 siRNA. Conversely, adenovirus-mediated overexpression of NOR1 induces Skp2 expression in VSMC and decreases protein abundance of its target p27. Transient transfection experiments establish that NOR1 transactivates the Skp2 promoter through a nerve growth factor-induced clone B response element (NBRE). Electrophoretic mobility shift and chromatin immunoprecipitation assays further revealed that NOR1 is recruited to this NBRE site in the Skp2 promoter in response to mitogenic stimulation. In vivo Skp2 expression is increased during the proliferative response underlying neointima formation, and this transcriptional induction depends on the expression of NOR1. Finally, we demonstrate that overexpression of Skp2 rescues the proliferative arrest of Nor1-deficient VSMC. Collectively, these results characterize Skp2 as a novel NOR1-regulated target gene and detail a previously unrecognized transcriptional cascade regulating mitogen-induced VSMC proliferation. PMID:21868379

  5. Diagnóstico do cumprimento da NR-18, pelas empresas construtoras da grande João Pessoa, vinte anos após sua revisão

    Directory of Open Access Journals (Sweden)

    Mirela Oliveira Medeiros

    2016-09-01

    Full Text Available A NR-18 é a primeira norma específica criada para uma indústria. Essa norma visa a melhoria das condições de trabalho na indústria da construção, tendo por objetivos a promoção da saúde e a melhoria da qualidade de vida do trabalhador e a prevenção de acidentes de trabalho. O artigo em foco é resultado de uma pesquisa exploratória descritiva que teve como objetivo elaborar um diagnóstico quanto ao cumprimento da NR-18 pelas empresas construtoras da grande João Pessoa, vinte anos após sua revisão. O universo da pesquisa foi composto pelas empresas construtoras de edificações verticais cadastradas junto ao Sindicato da Indústria da Construção Civil de João Pessoa (Sinduscon-JP. Já a amostra, esta foi composta por dezesseis empresas construtoras que aceitaram participar da pesquisa e que possuíam, no momento da pesquisa, canteiros de obras na grande João Pessoa. Os resultados revelam que as empresas construtoras têm buscado o efetivo cumprimento das disposições que compõem a NR-18 nos seus canteiros de obras. Constata-se que os canteiros pesquisados possuem nota média igual a 9, sendo a menor nota obtida 8,45, e que a interpretação da norma, para alguns itens, ainda é realizada de forma distinta pela fiscalização e pelos gestores.

  6. Enhanced Abscisic Acid-Mediated Responses in nia1nia2noa1-2 Triple Mutant Impaired in NIA/NR- and AtNOA1-Dependent Nitric Oxide Biosynthesis in Arabidopsis1[W

    Science.gov (United States)

    Lozano-Juste, Jorge; León, José

    2010-01-01

    Nitric oxide (NO) regulates a wide range of plant processes from development to environmental adaptation. Despite its reported regulatory functions, it remains unclear how NO is synthesized in plants. We have generated a triple nia1nia2noa1-2 mutant that is impaired in nitrate reductase (NIA/NR)- and Nitric Oxide-Associated1 (AtNOA1)-mediated NO biosynthetic pathways. NO content in roots of nia1nia2 and noa1-2 plants was lower than in wild-type plants and below the detection limit in nia1nia2noa1-2 plants. NIA/NR- and AtNOA1-mediated biosynthesis of NO were thus active and responsible for most of the NO production in Arabidopsis (Arabidopsis thaliana). The nia1nia2noa1-2 plants displayed reduced size, fertility, and seed germination potential but increased dormancy and resistance to water deficit. The increasing deficiency in NO of nia1nia2, noa1-2, and nia1nia2noa1-2 plants correlated with increased seed dormancy, hypersensitivity to abscisic acid (ABA) in seed germination and establishment, as well as dehydration resistance. In nia1nia2noa1-2 plants, enhanced drought tolerance was due to a very efficient stomata closure and inhibition of opening by ABA, thus uncoupling NO from ABA-triggered responses in NO-deficient guard cells. The NO-deficient mutants in NIA/NR- and AtNOA1-mediated pathways in combination with the triple mutant will be useful tools to functionally characterize the role of NO and the contribution of both biosynthetic pathways in regulating plant development and defense. PMID:20007448

  7. NR2B subunit-dependent long-term potentiation enhancement in the rat cortical auditory system in vivo following masking of patterned auditory input by white noise exposure during early postnatal life.

    Science.gov (United States)

    Hogsden, Jennifer L; Dringenberg, Hans C

    2009-08-01

    The composition of N-methyl-D-aspartate (NMDA) receptor subunits influences the degree of synaptic plasticity expressed during development and into adulthood. Here, we show that theta-burst stimulation of the medial geniculate nucleus reliably induced NMDA receptor-dependent long-term potentiation (LTP) of field postsynaptic potentials recorded in the primary auditory cortex (A1) of urethane-anesthetized rats. Furthermore, substantially greater levels of LTP were elicited in juvenile animals (30-37 days old; approximately 55% maximal potentiation) than in adult animals (approximately 30% potentiation). Masking patterned sound via continuous white noise exposure during early postnatal life (from postnatal day 5 to postnatal day 50-60) resulted in enhanced, juvenile-like levels of LTP (approximately 70% maximal potentiation) relative to age-matched controls reared in unaltered acoustic environments (approximately 30%). Rats reared in white noise and then placed in unaltered acoustic environments for 40-50 days showed levels of LTP comparable to those of adult controls, indicating that white noise rearing results in a form of developmental arrest that can be overcome by subsequent patterned sound exposure. We explored the mechanisms mediating white noise-induced plasticity enhancements by local NR2B subunit antagonist application in A1. NR2B subunit antagonists (Ro 25-6981 or ifenprodil) completely reversed white noise-induced LTP enhancement at concentrations that did not affect LTP in adult or age-matched controls. We conclude that white noise exposure during early postnatal life results in the maintenance of juvenile-like, higher levels of plasticity in A1, an effect that appears to be critically dependent on NR2B subunit activation.

  8. Efeito do óleo de linhaça e do óleo de amendoim sobre a vulcanização da borracha natural (NR. parte II: modelo detalhado Effect of linseed oil and peanut oil upon natural rubber (NR vulcanization. part II: detailed model

    Directory of Open Access Journals (Sweden)

    Alexandre Bezerra

    2013-01-01

    Full Text Available O efeito da substituição total ou parcial do ácido esteárico por óleos vegetais de linhaça e amendoim foi investigado em composições de borracha natural (NR. Um sistema de vulcanização convencional foi escolhido e os estudos cinéticos foram conduzidos nas temperaturas de 160 ºC, 170 ºC e 180 ºC. O modelo cinético detalhado permitiu estudar os diferentes estágios da vulcanização e verificar que na etapa de formação do precursor de ligações cruzadas (A → B houve diferenças significativas entre o ácido esteárico e os óleos vegetais. Tal resultado foi devido à presença expressiva de ácidos graxos insaturados na composição química dos óleos de linhaça e de amendoim (principalmente, os ácidos oléico e linolênico, o que facilitou a ocorrência de reações laterais inesperadas e, consequentemente, diminuiu o rendimento final de ligações cruzadas nos vulcanizados.The effect of the total or partial replacement of stearic acid with vegetable oils of linseed and peanut has been investigated in natural rubber (NR compositions. A conventional vulcanization system has been chosen and kinetic studies have been done in the temperatures of 160 ºC, 170 ºC and 180 ºC. The detailed kinetic model has allowed studying the different stages of vulcanization and verifying that in the formation of the crosslink precursor (A → B there were significant differences between stearic acid and the vegetable oils. This result has been attributed to the high concentration of unsaturated fat acids in the chemical composition of the vegetable oils (mainly, oleic and linolenic acids, which allowed unexpected side reactions and, consequently, diminished the final yield of crosslinking in the vulcanizates.

  9. Development of biotest assays using cell cultures from fish for the demonstration of lethal and sublethal damage to organisms due to environmental pollutants in water. Cellular biomarkers in fish cell cultures; Entwicklung von Biotestverfahren mit Zellkulturen aus Fischen zum Nachweis letaler und subletaler Schaeden von Organismen durch Umweltschadstoffe im Wasser. Zellulaere Biomarker in Fischzellkulturen

    Energy Technology Data Exchange (ETDEWEB)

    Braunbeck, T [Heidelberg Univ. (Germany). Zoologisches Inst. 1

    1994-06-01

    Methoden zum Nachweis des (oeko-)toxikologischen Potential von Schadstoffen dar. In allen Experimenten konnten klare Zeit-Wirkungs- und Dosis-Wirkungs-Beziehungen aufgestellt werden. Isolierte Hepatocyten aus der Leber der Regebogenforelle reagieren empfindlicher als die parallel untersuchten permanenten Zellinien R1 und RTG-2. In Cytotoxizitaetstests mit den bibrocytischen Fischzellinien R1 und RTG-2 aus der Leber bzw. der Gonade der Regenbogenforelle wurden fuer verschiedene organische Schadstoffe und organische und anorganische Schwermetallverbindungen Cytotoxizitaetsdaten erhoben. Diese Erkenntnisse wurden einem Konzept fuer den Einsatz von mehrstugigen Zelltests zur Abschaetzung des oekotoxikologischen Potentials von Umweltschadstoffen zugrunde gelegt, das hier vorgestellt wird. (orig./VHE)

  10. Reduction of {sup 137}caesium contamination in wild boars by supplementing offered food with ammonium-iron-hexa-cyanoferrate; Reduktion der {sup 137}Caesium-Aktivitaet in Wildschweinen durch Zusatz von Ammonium-Eisen-Hexacyanoferrat (''Berliner Blau'') zum Kirrfutter

    Energy Technology Data Exchange (ETDEWEB)

    Morfeld, P. [Evonik Industries AG, Essen (Germany). Inst. fuer Epidemiologie und Risikobewertung in der Arbeitswelt (IERA); Koeln Univ. (Germany). Inst. und Poliklinik fuer Arbeitsmedizin, Umweltmedizin und Praeventionsforschung; Reddemann, J.; Schungel, P. [Landesjagdverband Bayern e.V., Feldkirchen (Germany); Kienzle, E. [Muenchen Univ., Oberschleissheim (Germany). Lehrstuhl fuer Tierernaehrung und Diaetetik

    2014-06-15

    This replication study investigated whether the {sup 137}caesium ({sup 137}Cs) contamination of wild boars could be relevantly reduced under field conditions by adding ammonium-iron-hexa-cyanoferrate (AFCF; Prussian blue) to the food. In 285 wild boars that had been shot in six Bavarian hunting territories during the season (November until May) between 01 November 2010 and 10 December 2011 {sup 137}Cs contamination was analysed. Thirty-five animals originated from two hunting territories in which offered food had been supplemented with 1250 mg AFCF per kilogram food. The control animals showed a mean {sup 137}Cs contamination of 522 Bq/kg lean skeletal muscle meat. Direct (univariable) comparisons of the two experimental territories with the four control territories yielded a mean reduction in {sup 137}Cs contamination due to Prussian bluefeeding by -211 Bq/kg (p < 0.001). Multivariable mo dels that took potential confounders into account (age, weight, sex, hunting date, territory) estimated the effect to be -344 Bq/kg (p < 0.05). This replication study confirmed the finding of Kienzle et al. (12) who described a statistically significant reduction in {sup 137}Cs contamination by -380 Bq/kg due to the feeding of Prussian blue in other territories. [German] Gegenstand und Ziel: Diese Replikationsstudie pruefte, ob unter Feldbedingungen die Kontamination von Wildschweinen mit {sup 137}Caesium ({sup 137}Cs) durch Zugabe vn Ammonium-Eisen-Hexacyanoferrat (AFCF, ''Berliner Blau'') zum ausgelegten Futter relevant verringert werden kann. Material und Methoden: Bei 285 Wildschweinen, die zwischen dem 01.11.2010 und dem 10.12.2011 waehrend der Jagdsaison (November bis Mai) in sechs bayerischen Jagdrevieren erlegt worden waren, erfolgte eine Analyse der {sup 137}Cs-Aktivitaet. Von diesen Tieren stammten 35 aus zwei Revieren, in denen das ausgelegte Futter mit 1250 mg AFCF/kg Futter supplementiert worden war. Ergebnisse: Die Kontrolltiere wiesen eine

  11. Comparison of the corrosion resistance of DIN W. Nr. 1.4970 (15%Cr-15%Ni-1.2%Mo-Ti and ASTM F-138 (17%Cr-13%Ni-2.5%Mo austenitic stainless steels for biomedical applications

    Directory of Open Access Journals (Sweden)

    Maysa Terada

    2006-09-01

    Full Text Available The resistance to localised corrosion of the full austenitic 15%Cr-15%Ni-1.2%Mo titanium stabilized stainless steel (DIN W. Nr. 1.4970 was investigated by electrochemical methods including electrochemical impedance spectroscopy (EIS, potentiodynamic polarization and potentiostatic polarization measurements in a phosphate-buffered solution (PBS. The low carbon and non-stabilized austenitic stainless steel, AISI 316L (ASTM F-138, widely used for surgical implants, was also tested for comparison. The tests were conducted at room temperature after a stable potential had been reached. After the electrochemical measurements, the surfaces of the specimens were observed using SEM to evaluate the presence of pits. Potentiodynamic polarization results showed that both steels are prone to localized corrosion. Larger pits were found on the surface of AISI 316L specimens after the electrochemical tests. EIS response has indicated the duplex structure of the passive oxides. The results showed that the electrochemical behaviour of the DIN W. Nr. 1.4970 is better than of AISI 316L steel. Therefore, their application as an implant material may be considered.

  12. Radiosynthesis of (E)-N-(2-[{sup 11}C]methoxybenzyl)-3-phenyl-acrylamidine, a novel subnanomolar NR2B subtype-selective NMDA receptor antagonist

    Energy Technology Data Exchange (ETDEWEB)

    Thominiaux, Cyrille [Service Hospitalier Frederic Joliot, Departement de Recherche Medicale, CEA/DSV, 4 place du General Leclerc, F-91401 Orsay (France); Bruin, Beatrice de [Service Hospitalier Frederic Joliot, Departement de Recherche Medicale, CEA/DSV, 4 place du General Leclerc, F-91401 Orsay (France); Bramoulle, Yann [Service Hospitalier Frederic Joliot, Departement de Recherche Medicale, CEA/DSV, 4 place du General Leclerc, F-91401 Orsay (France); Hinnen, Francoise [Service Hospitalier Frederic Joliot, Departement de Recherche Medicale, CEA/DSV, 4 place du General Leclerc, F-91401 Orsay (France); Demphel, Stephane [Service Hospitalier Frederic Joliot, Departement de Recherche Medicale, CEA/DSV, 4 place du General Leclerc, F-91401 Orsay (France); Valette, Heric [Service Hospitalier Frederic Joliot, Departement de Recherche Medicale, CEA/DSV, 4 place du General Leclerc, F-91401 Orsay (France); Bottlaender, Michel [Service Hospitalier Frederic Joliot, Departement de Recherche Medicale, CEA/DSV, 4 place du General Leclerc, F-91401 Orsay (France); Besret, Laurent [Service Hospitalier Frederic Joliot, Departement de Recherche Medicale, CEA/DSV, 4 place du General Leclerc, F-91401 Orsay (France); Departement de Recherche Medicale, URA CEA/CNRS 2210, Service Hospitalier Frederic Joliot, CEA/DSV, 4 Place du General Leclerc, F-91401 Orsay (France); Kassiou, Michael [Department of PET and Nuclear Medicine, Royal Prince Alfred Hospital, Missenden Road, Camperdown, NSW 2050 (Australia); Department of Pharmacology, University of Sydney, NSW 2006 (Australia); Dolle, Frederic [Service Hospitalier Frederic Joliot, Departement de Recherche Medicale, CEA/DSV, 4 place du General Leclerc, F-91401 Orsay (France)]. E-mail: frederic.dolle@cea.fr

    2006-03-15

    Recently, a novel series of amidines has been described, exhibiting high NR2B-subtype selective N-methyl-D-aspartate (NMDA) antagonist activity with nanomolar or subnanomolar affinity. Within the styrylamidine subclass (E)-N-(2-methoxybenzyl)-3-phenyl-acrylamidine (1), displayed the highest affinity (Ki=0.7nM versus [{sup 3}H]ifenprodil) and was considered an appropriate candidate for isotopic labelling with carbon-11 (T{sub 1/2}: 20.38min) at its methoxy group for imaging of NMDA receptors with PET. Derivative 1 has been labelled from the corresponding nor-analogue using [{sup 11}C]methyl triflate and the following experimental conditions : (1) trapping at -10{sup o}C of [{sup 11}C]methyl triflate in 300{mu}L of acetone containing 0.6-0.8mg of precursor 5 (2.4-3.2{mu}mol) and 5{mu}L of a 3M solution of NaOH in water (about 5eq.); (2) concentration to dryness of the reaction mixture (at 110{sup o}C, using a helium stream for 1-2min); (3) taking up the residue with 0.5mL of the HPLC mobile phase and (4) purification using semi-preparative HPLC (SymmetryPrep{sup (}R) C-18, Waters, 300x7.8mm). Typically, starting from a 1.5 Ci (55.5GBq) [{sup 11}C]CO{sub 2} production batch, 120-240m Ci (4.44-8.88GBq) of [{sup 11}C]-1 (20-40% decay-corrected radiochemical yield, n=5) was obtained within a total synthesis time of 25-30min. Specific radioactivities ranged from 0.8 to 1.2Ci/{mu}mol (29.6-44.4GBq/{mu}mol) at the end of radiosynthesis. No attempts were made to further optimise these reactions, as sufficient material was obtained to allow for preliminary pharmacological characterisation.

  13. Sustainable water management and regional development. Analysis and assessment of scenarios on conflicts of water use in the Upper Spree catchment area, a region impacted by brown coal mining; Nachhaltige Wasserbewirtschaftung und regionale Entwicklung. Analyse und Bewertung von Szenarien zum Wassernutzungskonflikt im bergbaubeeinflussten Einzugsgebiet der Oberen Spree

    Energy Technology Data Exchange (ETDEWEB)

    Messner, F. [UFZ - Umweltforschungszentrum Leipzig-Halle GmbH, Leipzig (Germany). Sektion Oekonomie, Soziologie und Recht; Kaltofen, M. (eds.) [Brandenburgische Technische Univ. Cottbus (Germany)]|[Gesellschaft fuer Wasserwirtschaftliche Planung und Systemforschung mbH (WASY), Berlin (Germany)

    2004-07-01

    , Wassernutzungskonflikten und daraus resultierenden Umwelt- und soziooekonomischen Problemen im Elbeeinzugsgebiet. Zur Erfuellung dieser Fragestellung wurde der Integrative Methodische Ansatz von GLOWA-Elbe (IMA) entwickelt und angewendet. Untersuchungsraeume waren das Gesamtflussgebiet der Elbe inklusive einer Intensivstudie im Unstrut-Flussgebiet mit Bezug auf die Wasserqualitaetsproblematik sowie die Teilraeume Obere Spree, Spreewald (vgl. Dietrich et al. 2004) und Berlin (vgl. Finke et al. 2003) zur Analyse des besonders bedeutsamen Oberflaechenwasserverfuegbarkeitskonflikts im Spreeeinzugsgebiet. Das Teilgebietsprojekt Obere Spree beschaeftigte sich im Rahmen von GLOWA Elbe mit der integrierten Analyse und Bewertung des Obere-Spree-Gebietes, in dem bedingt durch jahrzehntelange Nutzung als Braunkohleabbaugebiet einer der groessten Oberflaechenwassermengen- und -qualitaetskonflikte in der Bundesrepublik Deutschland zu bewaeltigen ist. Der IMA wurde im Kontext dieses Teilgebietprojektes entwickelt und getestet. In diesem Ergebnisbericht werden die wesentlichen wasserwirtschaftlichen und sozio-oekonomischen Ergebnisse dieses Teilgebietsprojektes dargelegt, wobei verschiedene Ergebnisse aus anderen Teilprojekten von GLOWA Elbe massgeblich verarbeitet wurden. Ausfuehrlichere Darlegungen zu Einzelaspekten des GLOWA-Elbe-Projektes sind in Publikationen nachzulesen, die andernorts erschienen sind. Zu verweisen ist in diesem Zusammenhang auf die Homepage von GLOWA Elbe (http://www.glowa-elbe.de) sowie auf den PIK-Report 2004. In diesem Bericht wird auf weiterfuehrende Literatur zum Teilgebietsprojekt Obere Spree jeweils an gegebenem Ort verwiesen. (orig.)

  14. Scientists help children victims of the Chernobyl reactor accident. Report on project phase 1 and annex to the report on phase 1: 1.4.1993 - 31.3.1996; Wissenschaftler helfen Tschernobyl-Kindern. Bericht der Phase I und Anhang zum Bericht der Phase I: 1.4.1993 - 31.3.1996

    Energy Technology Data Exchange (ETDEWEB)

    Reiners, C [Wuerzburg Univ. (Germany). Klinik und Poliklinik fuer Strahlentherapie; Streffer, C [Essen Univ. (Gesamthochschule) (Germany). Inst. fuer Medizinische Strahlenbiologie; Voigt, G; Paretzke, H G [GSF - Forschungszentrum fuer Umwelt und Gesundheit Neuherberg GmbH, Oberschleissheim (Germany). Inst. fuer Strahlenschutz; Heinemann, G [Preussische Elektrizitaets-AG (Preussenelektra), Hannover (Germany); Pfob, H [Badenwerk AG, Karlsruhe (Germany)

    1997-12-01

    Gemeinschaftsausschusses Strahlenforschung stand. Im Rahmen des Teilprojekts `Therapie und Ausbildung` wurden in der Zeit vom 01.04.1993-31.03.1996 insgesamt 99 Kinder aus Weissrussland mit besonders fortgeschrittenen Formen des Schilddruesenkrebses in Deutschland einer Spezialbehandlung mit Radioiod unterzogen. Bei rund 60% dieser Kinder konnte bisher eine komplette Beseitigung des Schilddruesenkrebses erreicht werden. Im Berichtzeitraum wurden ausserdem insgesamt 41 weissrussische Aerzte und Physiker auf dem Gebiete der nuklearmedizinischen Diagnostik und Therapie des Schilddruesenkarzinoms ausgebildet. Das Teilprojekt `Biologische Dosimetrie` befasst sich einerseits mit der Frage, ob die Bestimmung von Mikronuklei in peripheren Lymphozyten auch noch mehrere Jahre nach der Reaktorkatastrophe eine Aussage zur Strahlenexposition erlaubt. Ausserdem wurde geprueft, ob Mikronuklei geeignet sind, den Verlauf der Radioiodtherapie im Rahmen der Behandlung von Schilddruesentumoren zu verfolgen. Weiterhin wurde an einer im Vergleich zur vorliegenden Literatur grossen Zahl von kindlichen Tumoren geprueft, ob es spezifische Mutationsmuster in Tumor-Suppressorgenen (p-53) in Schilddruesentumoren gibt, die zum Nachweis der Strahleninduktion dieser Tumoren geeignet sich. Das Teilprojekt `Retrospektive Dosimetrie und Risikoanalyse` befasste sich vor allem mit der Frage, ob die Vermutung zutrifft, dass die Freisetzung von I-131 bei der Reaktorkatastrophe zur erhoehten Inzidenz von Schilddruesenkarzinomen bei Kindern gefuehrt hat. Das Teilprojekt `Koordinations- und Untersuchungsstelle Minsk` setzte die Aufgaben einer Dienstleistungsstelle vor Ort fuer alle am GAST-Projekt beteiligten Aktivitaeten um. (orig./MG)

  15. Conformidade do espaço interno livre de tratores agrícolas e itens de segurança obrigatórios segundo as normas NBR/ISO 4252 e NR 12

    OpenAIRE

    Gessieli Possebom; Jairo Luís Zanon Peripolli; Airton dos Santos Alonço

    2017-01-01

    Os tratores são máquinas agrícolas que possibilitam o aumento da produção de alimento em maiores áreas. Entretanto, para haver aproveitamento dessa ferramenta, os tratores devem seguir especificações técnicas que garantam melhores condições ergonômicas ao operador a fim de reduzir riscos de acidentes e melhorar a eficiência do trabalho. Assim, o objetivo deste estudo foi verificar se tais máquinas se enquadram nas normas técnicas NBR/ISO 4252 e NR 12, que definem as medidas do espaço livre in...

  16. Emerging Trends in Applied Mathematics: Dedicated to the Memory of Sir Asutosh Mookerjee and Contributions of S.N. Bose, M.N. Saha and N.R. Sen

    CERN Document Server

    Basu, Uma; De, Soumen

    2015-01-01

    The book is based on research presentations at the international conference, “Emerging Trends in Applied Mathematics: In the Memory of Sir Asutosh Mookerjee, S.N. Bose, M.N. Saha, and N.R. Sen”, held at the Department of Applied Mathematics, University of Calcutta, during 12–14 February 2014. It focuses on various emerging and challenging topics in the field of applied mathematics and theoretical physics. The book will be a valuable resource for postgraduate students at higher levels and researchers in applied mathematics and theoretical physics. Researchers presented a wide variety of themes in applied mathematics and theoretical physics—such as emergent periodicity in a field of chaos; Ricci flow equation and Poincare conjecture; Bose–Einstein condensation; geometry of local scale invariance and turbulence; statistical mechanics of human resource allocation: mathematical modelling of job-matching in labour markets; contact problem in elasticity; the Saha equation; computational fluid dynamics with...

  17. Synthesis and characterization of tritium labeled N-((R)-1-((S)-4-(4-chlorophenyl)-4-hydroxy-3,3-dimethylpiperidin-1-yl)-3-methyl-1-oxobutan-2-yl)-3-sulfamoylbenzamide.

    Science.gov (United States)

    Hong, Yang; Hynes, John; Tian, Yuan; Balasubramanian, Balu; Bonacorsi, Samuel

    2015-08-01

    N-((R)-1-((S)-4-(4-chlorophenyl)-4-hydroxy-3,3-dimethylpiperidin-1-yl)-3-methyl-1-oxobutan-2-yl)-3-sulfamoylbenzamide is a potent C-C chemokine receptor 1 (CCR1) antagonist. The compound, possessing benzamide functionality, successfully underwent tritium/hydrogen (T/H) exchange with an organoiridium catalyst (Crabtree's catalyst). The labeling pattern in the product was studied with liquid chromatography-mass spectrometry, time-of-flight mass spectrometry, and (3) H-NMR. Overall, multiple labeled species were identified. In addition to the anticipated incorporation of tritium in the benzamide moiety, tritium labeling was observed in the valine portion of the molecule including substitution at its chiral carbon. Using authentic standards, liquid chromatography analysis of the labeled compound showed complete retention of stereochemical configuration. Copyright © 2015 John Wiley & Sons, Ltd.

  18. Development of SCAR marker specific to non-toxic Jatropha curcas L. and designing a novel multiplexing PCR along with nrDNA ITS primers to circumvent the false negative detection

    KAUST Repository

    Mastan, Shaik G.

    2011-05-10

    Jatropha curcas L., a multipurpose shrub, has acquired significant economic importance for its seed oil which can be converted to biodiesel an emerging alternative to petro-diesel. In addition to the commercial value, it is also having medicinal and even high nutritional value to use as animal fodder which is limited due to the toxicity. Development of molecular marker will enable to differentiate non-toxic from toxic variety of J. curcas in a mixed population and also for quality control since the toxic components of J. curcas has deleterious effect on animals. In the present study, the efforts were made to generate the specific SCAR marker for toxic and/or non-toxic J. curcas from RAPD markers. Among the markers specific for toxic and non-toxic varieties, four were selected, purified, cloned, sequenced, and designed primers out of which one set of primers NT-JC/SCAR I/OPQ15-F and R could able to discriminate the non-toxic with toxic Jatropha by giving expected 430 bp size amplification in non-toxic variety. Furthermore, novel multiplex PCR was designed using the nrDNA ITS primers to overcome the false negatives. Present work also demonstrates utility of the conserved regions of nrDNA coding genes in ruling out the artifacts in PCR-like false negatives frequently occur in SCAR due to various reasons. The specific SCAR markers generated in the present investigation will help to distinguish non-toxic from toxic varieties of J. curcas or vice versa, and isolated marker along with designed multiplex protocol has applications in quality control for selective cultivation of non-toxic variety and will also assist in breeding and molecular mapping studies. © 2011 Springer Science+Business Media, LLC.

  19. Foerdervorrichtung zum geneigten oder senkrechten Foerdern von Stueckgut

    OpenAIRE

    Westermann, T.

    1997-01-01

    The first conveyor consists of a driven conveyor belt with tightener and pressure piece (4,5) pressing the conveyor belt (1), depending on the size of the piece of goods (13), against the second conveyor in the form of a fixed, inflexible wall slide plate (3). When the conveyor belt is placed against the fixed wall, an air gap (14) of between 5 and 10 millimetres long is formed between the two conveyors. Openings in the top and bottom of the conveyor arrangement, between the conveyor belt and...

  20. From graphene to silicene; Vom Graphen zum Silicen

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, Lars [Spektrum.de, Heidelberg (Germany)

    2012-08-15

    Already in 1993 it was predicted quantum mechanically: In analogy to carbon also the chemically related silicon atom should form a thick sheet of hexagons. Presumably, this silicene would be even more appropriate than graphene for high-power electronics. But now physicists succeeded in the production of this material in the laboratory.