Sample records for zeta regulates interleukin-8-mediated

  1. Diacylglycerol kinase-zeta localization in skeletal muscle is regulated by phosphorylation and interaction with syntrophins.

    Abramovici, Hanan; Hogan, Angela B; Obagi, Christopher; Topham, Matthew K; Gee, Stephen H


    Syntrophins are scaffolding proteins that link signaling molecules to dystrophin and the cytoskeleton. We previously reported that syntrophins interact with diacylglycerol kinase-zeta (DGK-zeta), which phosphorylates diacylglycerol to yield phosphatidic acid. Here, we show syntrophins and DGK-zeta form a complex in skeletal muscle whose translocation from the cytosol to the plasma membrane is regulated by protein kinase C-dependent phosphorylation of the DGK-zeta MARCKS domain. DGK-zeta mutants that do not bind syntrophins were mislocalized, and an activated mutant of this sort induced atypical changes in the actin cytoskeleton, indicating syntrophins are important for localizing DGK-zeta and regulating its activity. Consistent with a role in actin organization, DGK-zeta and syntrophins were colocalized with filamentous (F)-actin and Rac in lamellipodia and ruffles. Moreover, extracellular signal-related kinase-dependent phosphorylation of DGK-zeta regulated its association with the cytoskeleton. In adult muscle, DGK-zeta was colocalized with syntrophins on the sarcolemma and was concentrated at neuromuscular junctions (NMJs), whereas in type IIB fibers it was found exclusively at NMJs. DGK-zeta was reduced at the sarcolemma of dystrophin-deficient mdx mouse myofibers but was specifically retained at NMJs, indicating that dystrophin is important for the sarcolemmal but not synaptic localization of DGK-zeta. Together, our findings suggest syntrophins localize DGK-zeta signaling complexes at specialized domains of muscle cells, which may be critical for the proper control of lipid-signaling pathways regulating actin organization. In dystrophic muscle, mislocalized DGK-zeta may cause abnormal cytoskeletal changes that contribute to disease pathogenesis.

  2. Vectorial secretion of interleukin-8 mediates autocrine signalling in intestinal epithelial cells via apically located CXCR1

    Rossi, Oriana; Karczewski, Jurgen; Stolte, Ellen H; Brummer, Robert J M; van Nieuwenhoven, Michiel A; Meijerink, Marjolein; van Neerven, Joost R J; van Ijzendoorn, Sven C D; van Baarlen, Peter; Wells, Jerry M


    BACKGROUND: In the intestinal mucosa, several adaptations of TLR signalling have evolved to avoid chronic inflammatory responses to the presence of commensal microbes. Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting

  3. Interleukin-8-mediated heterologous receptor internalization provides resistance to HIV-1 infectivity. Role of signal strength and receptor desensitization.

    Richardson, Ricardo M; Tokunaga, Kenzo; Marjoram, Robin; Sata, Tetsutaro; Snyderman, Ralph


    Human immunodeficiency virus type 1 (HIV-1) entry into CD4(+) cells requires the chemokine receptors CCR5 or CXCR4 as co-fusion receptors. We have previously demonstrated that chemokine receptors are capable of cross-regulating the functions of each other and, thus, affecting cellular responsiveness at the site of infection. To investigate the effects of chemokine receptor cross-regulation in HIV-1 infection, monocytes and MAGIC5 and rat basophilic leukemia (RBL-2H3) cell lines co-expressing the interleukin-8 (IL-8 or CXCL8) receptor CXCR1 and either CCR5 (ACCR5) or CXCR4 (ACXCR4) were generated. IL-8 activation of CXCR1, but not the IL-8 receptor CXCR2, cross-phosphorylated CCR5 and CXCR4 and cross-desensitized their responsiveness to RANTES (regulated on activation normal T cell expressed and secreted) (CCL5) and stromal derived factor (SDF-1 or CXCL12), respectively. CXCR1 activation internalized CCR5 but not CXCR4 despite cross-phosphorylation of both. IL-8 pretreatment also inhibited CCR5- but not CXCR4-mediated virus entry into MAGIC5 cells. A tail-deleted mutant of CXCR1, DeltaCXCR1, produced greater signals upon activation (Ca(2+) mobilization and phosphoinositide hydrolysis) and cross-internalized CXCR4, inhibiting HIV-1 entry. The protein kinase C inhibitor staurosporine prevented phosphorylation and internalization of the receptors by CXCR1 activation. Taken together, these results indicate that chemokine receptor-mediated HIV-1 cell infection is blocked by receptor internalization but not desensitization alone. Thus, activation of chemokine receptors unrelated to CCR5 and CXCR4 may play a cross-regulatory role in the infection and propagation of HIV-1. Since DeltaCXCR1, but not CXCR1, cross-internalized and cross-inhibited HIV-1 infection to CXCR4, the data indicate the importance of the signal strength of a receptor and, as a consequence, protein kinase C activation in the suppression of HIV-1 infection by cross-receptor-mediated internalization.

  4. The cyclic AMP response element modulator regulates transcription of the TCR zeta-chain

    Tenbrock, K; Kyttaris, VC; Ahlmann, M; Ehrchen, JA; Tolnay, M; Melkonyan, H; Mawrin, C; Roth, J; Sorg, C; Juang, YT; Tsokos, GC


    Systemic lupus erythematusus T cells display decreased amounts of TCR zeta mRNA that results in part from limited binding of the transcriptional enhancer Elf-1 to the TCR zeta promoter. We have identified a new cis-binding site for the cAMP response element (CRE) modulator (CREM) on the TCR zeta pro

  5. Regulation of the Water Channel Aquaporin-2 via 14-3-3 Theta (θ) and Zeta (ζ)

    Moeller, Hanne B; Slengerik-Hansen, Joachim; Aroankins, Takwa


    . With the exception of sigma (σ), all 14-3-3 isoforms were abundantly expressed in mouse kidney and mouse kidney collecting duct cells (mpkCCD14). Long-term treatment of mpkCCD14 cells with the type 2 vasopressin receptor agonist dDAVP increased mRNA and protein levels of AQP2 alongside 14-3-3 beta (β) and zeta (ζ...

  6. Serine 58 of 14-3-3zeta is a molecular switch regulating ASK1 and oxidant stress-induced cell death.

    Zhou, Jibin; Shao, Zhili; Kerkela, Risto; Ichijo, Hidenori; Muslin, Anthony J; Pombo, Celia; Force, Thomas


    Oxidant stress is a ubiquitous stressor with negative impacts on multiple cell types. ASK1 is a central mediator of oxidant injury, but while mechanisms of its inhibition, such as sequestration by 14-3-3 proteins and thioredoxin, have been identified, mechanisms of activation have remained obscure and the signaling pathways regulating this are not clear. Here, we report that phosphorylation of 14-3-3zeta at serine 58 (S58) is dynamically regulated in the cell and that the phosphorylation status of S58 is a critical factor regulating oxidant stress-induced cell death. Phosphorylation of S58 releases ASK1 from 14-3-3zeta, and ASK1 then activates stress-activated protein kinases, leading to cell death. While several members of the mammalian sterile 20 (Mst) family of kinases can phosphorylate S58 when overexpressed, we identify Ste20/oxidant stress response kinase 1 (SOK-1), an Mst family member known to be activated by oxidant stress, as a central endogenous regulator of S58 phosphorylation and thereby of ASK1-mediated cell death. Our findings identify a novel pathway that regulates ASK1 activation and oxidant stress-induced cell death.

  7. Degenerate Euler zeta function

    Kim, Taekyun


    Recently, T. Kim considered Euler zeta function which interpolates Euler polynomials at negative integer (see [3]). In this paper, we study degenerate Euler zeta function which is holomorphic function on complex s-plane associated with degenerate Euler polynomials at negative integers.

  8. Riemann Zeta Matrix Function

    Kargın, Levent; Kurt, Veli


    In this study, obtaining the matrix analog of the Euler's reflection formula for the classical gamma function we expand the domain of the gamma matrix function and give a infinite product expansion of sinπxP.  Furthermore we define Riemann zeta matrix function and evaluate some other matrix integrals. We prove a functional equation for Riemann zeta matrix function.

  9. Regulation of the Water Channel Aquaporin-2 via 14-3-3 Theta (θ) and Zeta (ζ)

    Moeller, Hanne B; Slengerik-Hansen, Joachim; Aroankins, Takwa


    The 14-3-3 family of proteins are multifunctional proteins that interact with many of their cellular targets in a phosphorylation-dependent manner. Here, we determined that 14-3-3 proteins interact with phosphorylated forms of the water channel aquaporin-2 (AQP2) and modulate its function....... With the exception of sigma (σ), all 14-3-3 isoforms were abundantly expressed in mouse kidney and mouse kidney collecting duct cells (mpkCCD14). Long-term treatment of mpkCCD14 cells with the type 2 vasopressin receptor agonist dDAVP increased mRNA and protein levels of AQP2 alongside 14-3-3 beta (β) and zeta (ζ......256 phosphorylation critical for the interactions. shRNA-mediated knockdown of 14-3-3 ζ in mpkCCD14 cells resulted in increased AQP2 ubiquitylation, decreased AQP2 protein half-life and reduced AQP2 levels. In contrast, knockdown of 14-3-3 θ resulted in increased AQP2 half-life and increased AQP2...

  10. Remarks on Shintani's zeta function

    Wakayama, Masato


    We introduce a zeta function attached to a representation of a group. We show that the multi-dimensional zeta function due to Shintani [Sh 1], which is a generalization of the multiple Hurwitz zeta function, can be obtained in this framework. We also construct a gamma function from the zeta function attached to a representation via zeta regularization. We study then a $q$-analogue of the Shintani zeta function and the corresponding gamma function. A sine function defined via the reflection fo...

  11. Bilateral zeta functions and their applications

    Shibukawa, Genki


    We introduce a new type of multiple zeta functions, which we call bilateral zeta functions, analogous to the Barnes zeta functions. The bilateral zeta function is a periodic function and shares certain basic properties of Barnes zeta function. Especially, we prove that the bilateral zeta function has a nice Fourier series expansion and the Barnes zeta function can be expressed as a finite sum of bilateral zeta functions. By these properties of the bilateral zeta functions, We obtain simple pr...

  12. Interpolation of multiple zeta and zeta-star values

    Yamamoto, Shuji


    We define polynomials of one variable t whose values at t=0 and 1 are the multiple zeta values and the multiple zeta-star values, respectively. We give an application to the two-one conjecture of Ohno-Zudilin, and also prove the cyclic sum formula for these polynomials.

  13. On a new zeta function

    Sun, Zhi-Wei


    Let m be a positive integer. We introduce a new zeta function zeta_m(s) defined by zeta_m(s) = sum_{n>0(-e^{2*pi*i/m})^{Omega(n)}/n^s for Re(s)>1, where Omega(n) denotes the total number of prime factors of n (counted with multiplicity). We show that sum_{n\\le x}(-e^{2*pi*i/3})^{Omega(n)}/n is asymptotically equivalent to c(log x)^{(1-sqrt{-3})/2} with c a nonzero complex number, and that sum_{n\\le x}(-e^{2*pi*i/m})^{Omega(n)}/n=O(1) for all m=4,5,6,... We note that zeta_m(s)*sum_{n>0}mu_m(n)/n^s=1 for Re(s)>1, where mu_m(n)=e^{2*pi*i*Omega(n)/m} if n is squarefree, and mu_m(n)=0 otherwise. We also prove that sum_{n>0}mu_3(n)/n=0, which is similar to the known identity sum_{n>0}mu(n)/n=0 equivalent to the Prime Number Theorem. In contrast with the Riemann Hypothesis, we raise the following new hypothesis: zeta_m(1):=sum_{n>0}(-e^{2*pi*i/m})^{Omega(n)}/n=0 for all m=5,6,....

  14. Bilateral zeta functions and their applications

    Shibukawa, Genki


    We introduce a new type of multiple zeta functions, which we call bilateral zeta functions, analogous to the Barnes zeta functions. The bilateral zeta function is a periodic function and shares certain basic properties of Barnes zeta function. Especially, we prove that the bilateral zeta function has a nice Fourier series expansion and the Barnes zeta function can be expressed as a finite sum of bilateral zeta functions. By these properties of the bilateral zeta functions, We obtain simple proofs of some formulas, for example the reflection formula for the multiple gamma function, the inversion formula of the Dedekind eta function, Ramanujan's formula, Fourier expansion of the Barnes zeta function and multiple Iseki's formula.

  15. Proof of Riemann's zeta-hypothesis

    Bergstrom, Arne


    Make an exponential transformation in the integral formulation of Riemann's zeta-function zeta(s) for Re(s) > 0. Separately, in addition make the substitution s -> 1 - s and then transform back to s again using the functional equation. Using residue calculus, we can in this way get two alternative, equivalent series expansions for zeta(s) of order N, both valid inside the "critical strip", i e for 0 < Re(s) < 1. Together, these two expansions embody important characteristics of the zeta-funct...

  16. Zeta regularized products, Riemann zeta zeros and prime number spectra

    Menezes, G; Svaiter, N F


    The Riemann hypothesis states that all nontrivial zeros of the zeta function lie in the critical line $\\Re(s)=1/2$. Hilbert and P\\'olya suggested that one possible way to prove the Riemann hypothesis is to interpret the nontrivial zeros in the light of spectral theory. Following this approach, we associate such a numerical sequence with the discrete spectrum of a linear differential operator. We discuss a necessary condition that such a sequence of numbers should obey in order to be associated with the spectrum of a linear differential operator of a system with countably infinite number of degrees of freedom. The sequence of nontrivial zeros is zeta regularizable. Then, functional integrals associated with hypothetical systems described by self-adjoint operators whose spectra is given by the sequence of the nontrivial zeros of the Riemann zeta function could be constructed. In addition, we demonstrate that if one considers the same situation with primes numbers, the associated functional integral cannot be co...

  17. Distinct regulation of p53-mediated apoptosis by protein kinase calpha, delta, epsilon and zeta : evidence in yeast for transcription-dependent and -independent p53 apoptotic mechanisms

    Coutinho, Isabel; Pereira, Clara; Pereira, Gil; Gonçalves, Jorge; Côrte-Real, Manuela; Saraiva, Lucília


    The role of individual protein kinase C (PKC) isoforms in the regulation of p53- mediated apoptosis is still uncertain. Using yeast cells co-expressing the human wild-type p53 and a single mammalian PKCa, d, e or z, we showed a differential regulation of p53- mediated apoptosis by these PKC isoforms. Whereas PKCa and z had no effect on p53 activity, PKCd and e stimulated a p53-mediated mitochondria-dependent apoptosis. Moreover, using pifithrin-a and -m, selective inhibitors of...

  18. Bernoulli numbers and zeta functions

    Arakawa, Tsuneo; Kaneko, Masanobu


    Two major subjects are treated in this book. The main one is the theory of Bernoulli numbers and the other is the theory of zeta functions. Historically, Bernoulli numbers were introduced to give formulas for the sums of powers of consecutive integers. The real reason that they are indispensable for number theory, however, lies in the fact that special values of the Riemann zeta function can be written by using Bernoulli numbers. This leads to more advanced topics, a number of which are treated in this book: Historical remarks on Bernoulli numbers and the formula for the sum of powers of consecutive integers; a formula for Bernoulli numbers by Stirling numbers; the Clausen–von Staudt theorem on the denominators of Bernoulli numbers; Kummer's congruence between Bernoulli numbers and a related theory of p-adic measures; the Euler–Maclaurin summation formula; the functional equation of the Riemann zeta function and the Dirichlet L functions, and their special values at suitable integers; various formulas of ...

  19. Convergence of zeta functions of graphs

    Clair, Bryan; Mokhtari-Sharghi, Shahriar


    The $L^2$-zeta function of an infinite graph Y (defined previously in a ball around zero) has an analytic extension. For a tower of finite graphs covered by Y, the normalized zeta functions of the finite graphs converge to the $L^2$-zeta function of Y.

  20. On multiple zeta values of even arguments

    Hoffmann, Michael E. [U.S. Naval Academy, Annapolis, MD (United States). Dept. of Mathematics; Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany)


    For k {<=} n, let E(2n,k) be the sum of all multiple zeta values with even arguments whose weight is 2n and whose depth is k. Of course E(2n,1) is the value {zeta}(2n) of the Riemann zeta function at 2n, and it is well known that E(2n,2)=(3)/(4){zeta}(2n). Recently Z. Shen and T. Cai gave formulas for E(2n,3) and E(2n,4) in terms {zeta}(2n) and {zeta}(2){zeta}(2n-2). We give two formulas form E(2n,k), both valid for arbitrary k{<=}n, one of which generalizes the Shen-Cai results; by comparing the two we obtain a Bernoulli-number identity. We also give an explicit generating function for the numbers E(2n,k).

  1. On differences of zeta values

    Flajolet, Philippe; Vepstas, Linas


    Finite differences of values of the Riemann zeta function at the integers are explored. Such quantities, which occur as coefficients in Newton series representations, have surfaced in works of Bombieri-Lagarias, Maslanka, Coffey, Báez-Duarte, Voros and others. We apply the theory of Nörlund-Rice integrals in conjunction with the saddle-point method and derive precise asymptotic estimates. The method extends to Dirichlet L-functions and our estimates appear to be partly related to earlier investigations surrounding Li's criterion for the Riemann hypothesis.

  2. Multiple q-Zeta Brackets

    Wadim Zudilin


    Full Text Available The multiple zeta values (MZVs possess a rich algebraic structure of algebraic relations, which is conjecturally determined by two different (shuffle and stuffle products of a certain algebra of noncommutative words. In a recent work, Bachmann constructed a q-analogue of the MZVs—the so-called bi-brackets—for which the two products are dual to each other, in a very natural way. We overview Bachmann’s construction and discuss the radial asymptotics of the bi-brackets, its links to the MZVs, and related linear (independence questions of the q-analogue.

  3. The triple system Zeta Aquarii

    Tokovinin, Andrei


    Zeta Aquarii is a bright and nearby (28 pc) triple star with a 26-year astrometric subsystem. Almost a half of the outer 540-year visual orbit has been covered in 238 years of its observations. Both inner and outer orbits are revised here taking into account recent direct resolution of the inner pair Aa,Ab. The inner orbit has a high eccentricity of 0.87 and is inclined to the outer orbit by 140+-10 degrees, suggesting that Kozai-Lidov cycles take place. The masses of the stars Aa, B, and Ab are 1.4, 1.4, and 0.6 solar. The age of the system is about 3 Gyr, and the two main components have just left the main sequence. Hypothetically, this system could have formed by a dynamical capture of the small star Ab in the twin binary Aa,B.

  4. Riemann zeta function is a fractal

    Woon, S C


    Voronin's theorem on the "Universality" of Riemann zeta function is shown to imply that Riemann zeta function is a fractal (in the sense that Mandelbrot set is a fractal) and a concrete "representation" of the "giant book of theorems'' that Paul Halmos referred to.

  5. Characterization of zeta (zeta): a new opioid receptor involved in growth.

    Zagon, I S; Goodman, S R; McLaughlin, P J


    Endogenous opioid systems (i.e., opioids and opioid receptors) are known to play a role in neural cancer. Using [3H]-[Met5]enkephalin, a potent ligand involved in growth, specific and saturable binding was detected in homogenates of S20Y neuroblastoma transplanted into A/Jax mice; the data fit a single binding site. Scatchard analysis yielded a Kd of 0.49 nM and a binding capacity of 5.32 fmol/mg protein. Binding was dependent on protein concentration, time, temperature, and pH, and was sensitive to Na+ and guanine nucleotides. Optimal binding required protease inhibitors, and pretreatment of the tumor homogenates with trypsin markedly reduced [3H]-[Met5]enkephalin binding, suggesting that the binding site was proteinaceous in character. Displacement experiments indicated that [Met5]enkephalin was the most potent displacer of [3H]-[Met5]enkephalin; other ligands selective for mu, delta, kappa, epsilon, and sigma were not highly competitive. Given the functional significance of [Met5]enkephalin as a potent regulator of normal and abnormal growth, and that the receptor recognized by [Met5]enkephalin does not resemble any previously described, the present study has demonstrated the presence of a new opioid receptor termed zeta (zeta) (from the Greek 'Zoe', life) related to the proliferation of cells and tissues.

  6. Integrals of products of Hurwitz zeta functions

    Shpot, M A; Paris, R B


    We evaluate two integrals over $x\\in [0,1]$ involving products of the function $\\zeta_1(a,x)\\equiv \\zeta(a,x)-x^{-a}$ for $\\Re (a)>1$, where $\\zeta(a,x)$ is the Hurwitz zeta function. The evaluation of these integrals for the particular case of integer $a\\geq 2$ is also presented. As an application we calculate the $O(g)$ weak-coupling expansion coefficient $c_{1}(\\varepsilon)$ of the Casimir energy for a film with Dirichlet-Dirichlet boundary conditions, first stated by Symanzik [Schr\\"odinger representation and Casimir effect in renormalizable quantum field theory, Nucl. Phys. B 190 (1981) 1-44] in the framework of $g\\phi^4_{4-\\varepsilon}$ theory.

  7. Interaction of nucleosome assembly proteins abolishes nuclear localization of DGK{zeta} by attenuating its association with importins

    Okada, Masashi; Hozumi, Yasukazu [Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990-9585 (Japan); Ichimura, Tohru [Department of Chemistry, Graduate School of Sciences and Engineering, Tokyo Metropolitan University, Hachioji 192-0397 (Japan); Tanaka, Toshiaki; Hasegawa, Hiroshi; Yamamoto, Masakazu; Takahashi, Nobuya [Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990-9585 (Japan); Iseki, Ken [Department of Emergency and Critical Care Medicine, Yamagata University School of Medicine, Yamagata 990-9585 (Japan); Yagisawa, Hitoshi [Laboratory of Biological Signaling, Graduate School of Life Science, University of Hyogo, Hyogo 678-1297 (Japan); Shinkawa, Takashi; Isobe, Toshiaki [Department of Chemistry, Graduate School of Sciences and Engineering, Tokyo Metropolitan University, Hachioji 192-0397 (Japan); Goto, Kaoru, E-mail: [Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990-9585 (Japan)


    Diacylglycerol kinase (DGK) is involved in the regulation of lipid-mediated signal transduction through the metabolism of a second messenger diacylglycerol. Of the DGK family, DGK{zeta}, which contains a nuclear localization signal, localizes mainly to the nucleus but translocates to the cytoplasm under pathological conditions. However, the detailed mechanism of translocation and its functional significance remain unclear. To elucidate these issues, we used a proteomic approach to search for protein targets that interact with DGK{zeta}. Results show that nucleosome assembly protein (NAP) 1-like 1 (NAP1L1) and NAP1-like 4 (NAP1L4) are identified as novel DGK{zeta} binding partners. NAP1Ls constitutively shuttle between the nucleus and the cytoplasm in transfected HEK293 cells. The molecular interaction of DGK{zeta} and NAP1Ls prohibits nuclear import of DGK{zeta} because binding of NAP1Ls to DGK{zeta} blocks import carrier proteins, Qip1 and NPI1, to interact with DGK{zeta}, leading to cytoplasmic tethering of DGK{zeta}. In addition, overexpression of NAP1Ls exerts a protective effect against doxorubicin-induced cytotoxicity. These findings suggest that NAP1Ls are involved in a novel molecular basis for the regulation of nucleocytoplasmic shuttling of DGK{zeta} and provide a clue to examine functional significance of its translocation under pathological conditions.

  8. Fourier coefficients associated with the Riemann zeta-function

    Y. V. Basiuk


    Full Text Available We study the Riemann zeta-function $\\zeta(s$ by a Fourier series method. The summation of $\\log|\\zeta(s|$ with the kernel $1/|s|^{6}$ on the critical line $\\mathrm{Re}\\; s = \\frac{1}{2}$ is the main result of our investigation. Also we obtain a new restatement of the Riemann Hypothesis.

  9. Partial zeta functions of algebraic varieties over finite fields

    Wan, D


    By restricting the variables running over various (possibly different) subfields, we introduce the notion of a partial zeta function. We prove that the partial zeta function is rational in an interesting case, generalizing Dwork's well known rationality theorem. In general, the partial zeta function is probably not rational. But a theorem of Faltings says that the partial zeta function is always nearly rational.

  10. Zeta functions in brane world cosmology

    Flachi, Antonino; Knapman, Alan; Naylor, Wade; Sasaki, Misao


    We present a calculation of the zeta function and of the functional determinant for a Laplace-type differential operator, corresponding to a scalar field in a higher-dimensional deSitter brane background, which consists of a higher-dimensional anti deSitter bulk spacetime bounded by a deSitter section, representing a brane. Contrary to the existing examples, which all make use of conformal transformations, we evaluate the zeta function working directly with the higher-dimensional wave operator. We also consider a generic mass term and coupling to curvature, generalizing previous results. The massless, conformally coupled case is obtained as a limit of the general result and compared with known calculations. In the limit of large anti deSitter radius, the zeta determinant for the ball is recovered in perfect agreement with known expressions, providing an interesting check of our result and an alternative way of obtaining the ball determinant.

  11. Zeta Functions Of Discrete Groups Acting On Trees

    Clair, Bryan; Mokhtari-Sharghi, Shahriar


    This paper generalizes Bass' work on zeta functions for uniform tree lattices. Using the theory of von Neumann algebras, machinery is developed to define the zeta function of a discrete group of automorphisms of a bounded degree tree. The main theorems relate the zeta function to determinants of operators defined on edges or vertices of the tree. A zeta function associated to a non-uniform tree lattice with appropriate Hilbert representation is defined. Zeta functions are defined for infinite...

  12. Euler Polynomials, Fourier Series and Zeta Numbers

    Scheufens, Ernst E


    Fourier series for Euler polynomials is used to obtain information about values of the Riemann zeta function for integer arguments greater than one. If the argument is even we recover the well-known exact values, if the argument is odd we find integral representations and rapidly convergent series....

  13. The magnetic field of zeta Orionis A

    Blazère, A; Tkachenko, A; Bouret, J -C; Rivinius, Th


    Zeta Ori A is a hot star claimed to host a weak magnetic field, but no clear magnetic detection was obtained so far. In addition, it was recently shown to be a binary system composed of a O9.5I supergiant and a B1IV star. We aim at verifying the presence of a magnetic field in zeta Ori A, identifying to which of the two binary components it belongs (or whether both stars are magnetic), and characterizing the field.Very high signal-to-noise spectropolarimetric data were obtained with Narval at the Bernard Lyot Telescope (TBL) in France. Archival HEROS, FEROS and UVES spectroscopic data were also used. The data were first disentangled to separate the two components. We then analyzed them with the Least-Squares Deconvolution (LSD) technique to extract the magnetic information. We confirm that zeta Ori A is magnetic. We find that the supergiant component zeta Ori Aa is the magnetic component: Zeeman signatures are observed and rotational modulation of the longitudinal magnetic field is clearly detected with a per...

  14. Euler Polynomials, Fourier Series and Zeta Numbers

    Scheufens, Ernst E


    Fourier series for Euler polynomials is used to obtain information about values of the Riemann zeta function for integer arguments greater than one. If the argument is even we recover the well-known exact values, if the argument is odd we find integral representations and rapidly convergent series....

  15. Bernoulli Polynomials, Fourier Series and Zeta Numbers

    Scheufens, Ernst E


    Fourier series for Bernoulli polynomials are used to obtain information about values of the Riemann zeta function for integer arguments greater than one. If the argument is even we recover the well-known exact values, if the argument is odd we find integral representations and rapidly convergent ...

  16. Zeta and q-Zeta Functions and Associated Series and Integrals

    Srivastava, H M


    Zeta and q-Zeta Functions and Associated Series and Integrals is a thoroughly revised, enlarged and updated version of Series Associated with the Zeta and Related Functions. Many of the chapters and sections of the book have been significantly modified or rewritten and a new chapter on the theory and applications of the basic (or q-) extensions of various Special Functions is included. This book will be invaluable as it covers not only detailed and systematic presentations of the theory and applications of the various methods and techniques used in dealing with many different classes of ser

  17. Special Uniformity of Zeta Functions I. Geometric Aspect

    Weng, Lin


    The special uniformity of zeta functions claims that pure non-abelian zeta functions coincide with group zeta functions associated to the special linear groups. Naturally associated are three aspects, namely, the analytic, arithmetic, and geometric aspects. In the first paper of this series, we expose intrinsic geometric structures of our zetas by counting semi-stable bundles on curves defined over finite fields in terms of their automorphism groups and global sections. We show that such a counting maybe read from Artin zetas which are abelian in nature. This paper also contains an appendix written by H. Yoshida, one of the driving forces for us to seek group zetas. In this appendix, Yoshida introduces a new zeta as a function field analogue of the group zeta for SL2 for number fields and establishes the Riemann Hypothesis for it.

  18. Graph Zeta function and gauge theories

    He, Yang-Hui


    Along the recently trodden path of studying certain number theoretic properties of gauge theories, especially supersymmetric theories whose vacuum manifolds are non-trivial, we investigate Ihara's Graph Zeta Function for large classes of quiver theories and periodic tilings by bi-partite graphs. In particular, we examine issues such as the spectra of the adjacency and whether the gauge theory satisfies the strong and weak versions of the graph theoretical analogue of the Riemann Hypothesis.

  19. Computing zeta functions of sparse nondegenerate hypersurfaces

    Sperber, Steven


    Using the cohomology theory of Dwork, as developed by Adolphson and Sperber, we exhibit a deterministic algorithm to compute the zeta function of a nondegenerate hypersurface defined over a finite field. This algorithm is particularly well-suited to work with polynomials in small characteristic that have few monomials (relative to their dimension). Our method covers toric, affine, and projective hypersurfaces and also can be used to compute the L-function of an exponential sum.

  20. Zeta potential of mica covered by colloid particles: a streaming potential study.

    Adamczyk, Zbigniew; Zaucha, Maria; Zembala, Maria


    The streaming potential of mica covered by monodisperse latex particles was measured using the parallel-plate channel, four-electrode cell. The zeta potential of latex bearing amidine charged groups was regulated by the addition of NaCl (10(-4)-10(-2) M) and MgCl(2) (10(-4)-10(-2) M) at a constant pH 5.5 and by the change in pH (4-12) at 10(-2) M NaCl. The size of the latex particles, determined by dynamic light scattering, varied between 502 and 540 nm for the above electrolyte concentration range. Mica sheets have been covered with latex particles under diffusion transport conditions. The latex coverage was regulated by the bulk suspension concentration in the channel and the deposition time. The coverage was determined, with a relative precision of 2%, by the direct enumeration of particles by optical microscopy and AFM. The streaming potential of mica was then determined for a broad range of particle coverage 0 < theta < 0.5, the particle-to-substrate zeta potential ratio zeta(p)/zeta(i), and 8.8 < kappa a < 143 (thin double-layer limit). These experimental data confirmed that the streaming potential of covered surfaces is well reflected by the theoretical approach formulated in ref 32. It was also shown experimentally that variations in the substrate streaming potential with particle coverage for theta < 0.3 and zeta(p)/zeta(i) < 0 are characterized by a large slope, which enables the precise detection of particles attached to interfaces. However, measurements at high coverage and various pH values revealed that the apparent zeta potential of covered surfaces is 1/2(1/2) smaller than the bulk zeta potential of particles (in absolute terms). This is valid for arbitrary zeta potentials of substrates and particles, including the case of negative particles on negatively charged substrates that mimics rough surfaces. Therefore, it was concluded that the streaming potential method can serve as an efficient tool for determining bulk zeta potentials of colloids and

  1. Zeta-functions of renormalizable sub-Lorenz templates

    Franco, Nuno, E-mail: nmf@uevora.p [CIMA-UE and Department of Mathematics, University of Evora, Rua Romao Ramalho, 59, 7000-671 Evora (Portugal); Silva, Luis, E-mail: lfs@dec.isel.ipl.p [CIMA-UE and Scientific Area of Mathematics, Instituto Superior de Engenharia de Lisboa, Rua Conselheiro Emidio Navarro, 1, 1959-007 Lisboa (Portugal)


    We describe the Williams zeta-functions and the twist zeta-functions of sub-Lorenz templates generated by renormalizable Lorenz maps, in terms of the corresponding zeta-functions of the sub-Lorenz templates generated by the renormalized map and by the map that determines the renormalization type.

  2. A study on the zeta potential of microcapsules during ageing.

    Labhasetwar, V D; Dorle, A K


    Gelatin, methylcellulose and agar microcapsules were prepared with and without suphadiazine. The zeta potential of these microcapsules was measured at regular intervals during ageing at 45 degrees C. An initial sharp rise in zeta potential is followed by a progressive decrease. Zeta potential could prove to be a useful parameter to study the changes occurring in the encapsulating material of microcapsules during ageing.

  3. Identification and characterization of the interaction between tuberin and 14-3-3zeta.

    Nellist, Mark; Goedbloed, Miriam A; de Winter, Christa; Verhaaf, Brenda; Jankie, Anita; Reuser, Arnold J J; van den Ouweland, Ans M W; van der Sluijs, Peter; Halley, Dicky J J


    Tuberous sclerosis is caused by mutations to either the TSC1 or TSC2 tumor suppressor gene. The disease is characterized by a broad phenotypic spectrum that includes seizures, mental retardation, renal dysfunction, and dermatological abnormalities. TSC1 encodes a 130-kDa protein called hamartin, and TSC2 encodes a 200-kDa protein called tuberin. Although it has been shown that hamartin and tuberin form a complex and mediate phosphoinositide 3-kinase/Akt-dependent phosphorylation of the ribosomal protein S6, it is not yet clear how inactivation of either protein leads to tuberous sclerosis. Therefore, to obtain additional insight into tuberin and hamartin function, yeast two-hybrid screening experiments were performed to identify proteins that interact with tuberin. One of the proteins identified was 14-3-3zeta, a member of the 14-3-3 protein family. The interaction between tuberin and 14-3-3zeta was confirmed in vitro and by co-immunoprecipitation; multiple sites within tuberin for 14-3-3zeta binding were identified; and it was determined that 14-3-3zeta associated with the tuberin-hamartin complex. Finally, it was shown that the tuberin/14-3-3zeta interaction is regulated by Akt-mediated phosphorylation of tuberin, providing insight into how tuberin may regulate phosphorylation of S6.

  4. Zeta potential in colloid science principles and applications

    Hunter, Robert J; Rowell, R L


    Zeta Potential in Colloid Science: Principles and Applications covers the concept of the zeta potential in colloid chemical theory. The book discusses the charge and potential distribution at interfaces; the calculation of the zeta potential; and the experimental techniques used in the measurement of electrokinetic parameters. The text also describes the electroviscous and viscoelectric effects; applications of the zeta potential to areas of colloid science; and the influence of simple inorganic ions or more complex adsorbates on zeta potential. Physical chemists and people involved in the stu

  5. An integral involving the generalized zeta function

    E. Elizalde


    Full Text Available A general value for ∫abdtlogΓ(t, for a, b positive reals, is derived in terms of the Hurwitz ζ function. That expression is checked for a previously known special integral, and the case where a is a positive integer and b is half an odd integer is considered. The result finds application in calculating the numerical value of the derivative of the Riemann zeta function at the point −1, a quantity that arises in the evaluation of determinants of Laplacians on compact Riemann surfaces.

  6. Modeling the Circumstellar Disk of $\\zeta$ Tauri

    Carciofi, A. C.; Bjorkman, J. E.


    We present a model for the disk of the classical Be star $\\zeta$ Tauri. The model consists of a Keplerian rotating disk with a power-law surface density and a vertical density distribution that follows from the balance between the thermal gas pressure and the z-component of the stellar gravitation. The opening angle of such a disk is not a fixed value but increases with the distance to the star (flared disk). We use a Monte Carlo code that solves simultaneously the thermal equilibrium, the st...



    A vanishing theorem is proved for -adic cohomology with compact support on an affine (singular) complete intersection. As an application, it is shown that for an affine complete intersection defined over a finite field of q elements, the reciprocal "poles" of the zeta function are always divisible by q as algebraic integers. A p-adic proof is also given, which leads to further q-divisibility of the poles or equivalently an improvement of the polar part of the AxKatz theorem for an affine complete intersection. Similar results hold for a projective complete intersection.

  8. Dedekind zeta-functions and Dedekind sums

    陆洪文; 焦荣政; 纪春岗


    In this paper we use Dedekind zeta functions of two real quadratic number fields at -1 to denote Dedekind sums of high rank. Our formula is different from that of Siegel's. As an application, we get a polynomial representation of ζK(-1): ζK(-1) =1/45(26n3-41n±9), n ≡±2(mod 5), where K=Q( q),prime q=4n2+1, and the class number of quadratic number field K2=Q(q) is 1.

  9. Zeta function factorisation, Dwork hypersurfaces, hypergeometric hypersurfaces

    Goutet, Philippe


    Let $\\mathbb{F}_q$ be a finite field with $q$ elements, $\\psi$ a non-zero element of $\\mathbb{F}_q$, and $n$ an integer $\\geq 3$ prime to $q$. The aim of this article is to show that the zeta function of the projective variety over $\\mathbb{F}_q$ defined by $X_\\psi \\colon x_1^n+...+x_n^n - n \\psi x_1... x_n=0$ has, when $n$ is prime and $X_\\psi$ is non singular (i.e. when $\\psi^n \

  10. Ten physical applications of spectral zeta functions

    Elizalde, Emilio


    Zeta-function regularization is a powerful method in perturbation theory. This book is meant as a guide for the student of this subject. Everything is explained in detail, in particular the mathematical difficulties and tricky points, and several applications are given to show how the procedure works in practice (e.g. Casimir effect, gravity and string theory, high-temperature phase transition, topological symmetry breaking). The formulas some of which are new can be used for accurate numerical calculations. The book is to be considered as a basic introduction and a collection of exercises for those who want to apply this regularization procedure in practice.

  11. Wettability Studies Using Zeta Potential Measurements

    Ghada Bassioni


    Full Text Available Wettability studies have been carried out on reservoir rocks using different techniques such as the Amott-Harvey method, the USBM method, and the contact angle method, all with limitations. In this study, the wettability is studied by discussing the surface charge using zeta potential measurements. The study relies on the finding that carbonated reservoir rocks, consisting of CaCO3 mainly, are positively charged and their surface has the potential to adsorb significant quantities of anions. Moreover, heavy fractions such as asphaltenes are reported to remain afloat depending on dispersive forces present in the oil and its various fractions. Experiments are carried out on aqueous limestone suspension with the addition of crude oil. The experiment is repeated with the use of polymeric inhibitors, A and B. The zeta potential is found to alter depending on the sequence of polymeric inhibitor in oil/water addition. The inhibitor is found to adsorb on the limestone surface, with a net negative charge, causing repulsion between crude oil and the inhibitor and, hence, preventing the deposition of heavy fractions and particularly asphaltenes. This study gives a comprehensive insight on the mechanism of polymeric inhibitor interaction with the surface and the effect of wettability on its performance.

  12. Los Zetas and Proprietary Radio Network Development

    James Halverson


    Full Text Available The years from 2006 through 2011 were very active years for a number of Mexican drug trafficking organizations. However, the group that probably saw the most meteoric rise in this period, Los Zetas, had a unique and innovative tool at their disposal. It was during these years that the group constructed and utilized a proprietary encrypted radio network that grew to span from Texas to Guatemala through the Gulf States of Mexico and across much of the rest of the country. This network gave the group an operational edge. It also stood as a symbol of the latitude the group enjoyed across vast areas, as this extensive illicit infrastructure stood, in the face of the government and rival cartels, for six years. This investigation explicates the process by which Los Zetas constructed, concealed and utilized this network and attempts to draw conclusions about the motivations and organizational dynamics that brought the network to be, with attention paid to what this case says about the complex engineering capabilities of non-state entities in general.

  13. Zeta-spectroscopy beyond 40 GeV

    Buchmueller, W.


    The zeta-family of bound states, formed by the anticipated t-quark and its antiquark, is discussed. The quantitative connection between the zeta-spectroscopy and the short distance behavior of the quark-antiquark potential is examined. It is pointed out that the next quarkonium system will lead to an accurate determination of the QCD scale parameter ..lambda... Weak zeta-decays are briefly considered.

  14. Fundamental Domains of Gamma and Zeta Functions

    Cabiria Andreian Cazacu


    Full Text Available Branched covering Riemann surfaces (ℂ,f are studied, where f is the Euler Gamma function and the Riemann Zeta function. For both of them fundamental domains are found and the group of cover transformations is revealed. In order to find fundamental domains, preimages of the real axis are taken and a thorough study of their geometry is performed. The technique of simultaneous continuation, introduced by the authors in previous papers, is used for this purpose. Color visualization of the conformal mapping of the complex plane by these functions is used for a better understanding of the theory. A version of this paper containing colored images can be found in arXiv at Andrian Cazacu and Ghisa.

  15. Zeta Functions and the Casimir Energy

    Blau, Steven K; Wipf, Andreas; 10.1016/0550-3213(88)90059-4


    We use zeta function techniques to give a finite definition for the Casimir energy of an arbitrary ultrastatic spacetime with or without boundaries. We find that the Casimir energy is intimately related to, but not identical to, the one-loop effective energy. We show that in general the Casimir energy depends on a normalization scale. This phenomenon has relevance to applications of the Casimir energy in bag models of QCD. Within the framework of Kaluza-Klein theories we discuss the one-loop corrections to the induced cosmological and Newton constants in terms of a Casimir like effect. We can calculate the dependence of these constants on the radius of the compact dimensions, without having to resort to detailed calculations.

  16. The multiple zeta value data mine

    Buemlein, J. [Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany); Broadhurst, D.J. [Open Univ., Milton Keynes (United Kingdom). Physics and Astronomy Dept.; Vermaseren, J.A.M. [Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany); NIKHEF, Amsterdam (Netherlands)


    We provide a data mine of proven results for multiple zeta values (MZVs) of the form {zeta}(s{sub 1},s{sub 2},..,s{sub k}) = sum {sup {infinity}}{sub n{sub 1}}{sub >n{sub 2}}{sub >...>n{sub k}}{sub >0} {l_brace}1/(n{sub 1}{sup s{sub 1}}..n{sub k}{sup s{sub k}}){r_brace} with weight w = sum {sup K}{sub i=1}s{sub i} and depth k and for Euler sums of the form sum {sup {infinity}}{sub n{sub 1}}{sub >n{sub 2}}{sub >...>n{sub k}}{sub >0} {l_brace}({epsilon}{sub 1}{sup n{sub 1}}..{epsilon}{sub 1}{sup n{sub k}})/(n{sub 1}{sup s{sub 1}}..n{sub k}{sup s{sub k}}){r_brace} with signs {epsilon}{sub i} = {+-} 1. Notably, we achieve explicit proven reductions of all MZVs with weights w{<=}22, and all Euler sums with weights w{<=}12, to bases whose dimensions, bigraded by weight and depth, have sizes in precise agreement with the Broadhurst. Kreimer and Broadhurst conjectures. Moreover, we lend further support to these conjectures by studying even greater weights (w{<=}30), using modular arithmetic. To obtain these results we derive a new type of relation for Euler sums, the Generalized Doubling Relations. We elucidate the ''pushdown'' mechanism, whereby the ornate enumeration of primitive MZVs, by weight and depth, is reconciled with the far simpler enumeration of primitive Euler sums. There is some evidence that this pushdown mechanism finds its origin in doubling relations. We hope that our data mine, obtained by exploiting the unique power of the computer algebra language FORM, will enable the study of many more such consequences of the double-shuffle algebra of MZVs, and their Euler cousins, which are already the subject of keen interest, to practitioners of quantum field theory, and to mathematicians alike. (orig.)

  17. A pseudo zeta function and the distribution of primes.

    Chernoff, P R


    The Riemann zeta function is given by: [equation, see published text]. Zeta(s) may be analytically continued to the entire s-plane, except for a simple pole at s = 0. Of great interest are the complex zeros of zeta(s). The Riemann hypothesis states that the complex zeros all have real part 1/2. According to the prime number theorem, pn approximately n logn, where pn is the nth prime. Suppose that pn were exactly nlogn. In other words, in the Euler product above, replace the nth prime by nlogn. In this way, we define a pseudo zeta function C(s) for Re s > 1. One can show that C(s) may be analytically continued at least into the half-plane Re s > 0 except for an isolated singularity (presumably a simple pole) at s = 0. It may be shown that the pseudo zeta function C(s) has no complex zeros whatsoever. This means that the complex zeros of the zeta function are associated with the irregularity of the distribution of the primes.

  18. Zeta Functions for Elliptic Curves I. Counting Bundles

    Weng, Lin


    To count bundles on curves, we study zetas of elliptic curves and their zeros. There are two types, i.e., the pure non-abelian zetas defined using moduli spaces of semi-stable bundles, and the group zetas defined for special linear groups. In lower ranks, we show that these two types of zetas coincide and satisfy the Riemann Hypothesis. For general cases, exposed is an intrinsic relation on automorphism groups of semi-stable bundles over elliptic curves, the so-called counting miracle. All this, together with Harder-Narasimhan, Desale-Ramanan and Zagier's result, gives an effective way to count semi-stable bundles on elliptic curves not only in terms of automorphism groups but more essentially in terms of their $h^0$'s. Distributions of zeros of high rank zetas are also discussed.

  19. On $p$-adic Hurwitz-type Euler zeta functions

    Kim, Min-Soo


    Henri Cohen and Eduardo Friedman constructed the $p$-adic analogue for Hurwitz zeta functions, and Raabe-type formulas for the $p$-adic gamma and zeta functions from Volkenborn integrals satisfying the modified difference equation. In this paper, we define the $p$-adic Hurwitz-type Euler zeta functions. Our main tool is the fermionic $p$-adic integral on $\\mathbb Z_p$. We find that many interesting properties for the $p$-adic Hurwitz zeta functions are also hold for the $p$-adic Hurwitz-type Euler zeta functions, including the convergent Laurent series expansion, the distribution formula, the functional equation, the reflection formula, the derivative formula, the $p$-adic Raabe formula and so on.

  20. X-ray in Zeta-Ori

    López-García, M. A.; López-Santiago, J. L.; Albacete-Colombo, J. F.; De Castro, E.


    Nearby star-forming regions are ideal laboratories to study high-energy emission processes but they usually present high absorption what makes difficult to detect the stellar population inside the molecular complex. As young late-type stars show high X-ray emission and X-ray photons are little absorbed by interstellar material, X-ray dedicated surveys are an excellent tool to detect the low-mass stellar population in optically absorbed regions. In this work, we present a study of the star-forming region Zeta-Ori and its surroundings. We combine optical, infrared and X-ray data. Properties of the X-ray emiting plasma and infrared features of the young stellar objects detected in the XMM-Newton observation are determined. The southern part of the Orion B giant molecular cloud complex harbor other star forming regions, as NGC 2023 and NGC 2024, we use this regions to compare. We study the spectral energy distribution of X-ray sources. Combining these results with infrared, the X-ray sources are classified as class I, class II and class III objects. The X-ray spectrum and ligth curve of detected X-ray sources is analyzed to found flares. We use a extincion-independent index to select the stars with circumstellar disk, and study the relationship between the present of disk and the flare energy. The results are similar to others studies and we conclude that the coronal properties of class II and class III objects in this region do not differ significantly from each other and from stars of similar infrared class in the ONC.

  1. Generation of directional EOF by interactive oscillatory zeta potential.

    Kuo, Chih-Yu; Wang, Chang-Yi; Chang, Chien-Cheng


    A steady directional EOF due to a nonlinear interaction between oscillatory axial electrical fields and oscillatory wall potentials (zeta potentials) is presented. This is a new mechanism to produce such a mean flow. It is found that the flow velocity depends not on the external driving frequency but on the phase angle difference between the electric fields and the zeta potentials. The formulation can also be reduced to the static EOF straightforwardly. For the purpose of theoretical demonstration, we use the Debye-Huckel approximation for the zeta potential. Results of planar and cylindrical capillaries are given.

  2. A study on zeta potential and dielectric constant of liposomes.

    Labhasetwar, V; Mohan, M S; Dorle, A K


    Zeta potential and dielectric constant of the liposomes were measured to study the effect of some of the formulation factors and in vitro ageing. Sonication affects zeta potential and dielectric constant of the liposomes. The ageing study showed an increase in the dielectric constant and zeta potential of liposomes at different storage temperatures. These two electrical parameters could be useful in studying structural alterations in liposomal vesicles and system as a function of different conditions. Particle size distribution and optical density were also measured, for comparison.

  3. An introduction to the theory of local zeta functions

    Igusa, Jun-ichi


    This book is an introductory presentation to the theory of local zeta functions. Viewed as distributions, and mostly in the archimedean case, local zeta functions are also called complex powers. The volume contains major results on analytic and algebraic properties of complex powers by Atiyah, Bernstein, I. M. Gelfand, S. I. Gelfand, and Sato. Chapters devoted to p-adic local zeta functions present Serre's structure theorem, a rationality theorem, and many examples found by the author. The presentation concludes with theorems by Denef and Meuser.

  4. Dynamics and zeta functions on conformally compact manifolds

    Rowlett, Julie; Tapie, Samuel


    In this note, we study the dynamics and associated zeta functions of conformally compact manifolds with variable negative sectional curvatures. We begin with a discussion of a larger class of manifolds known as convex co-compact manifolds with variable negative curvature. Applying results from dynamics on these spaces, we obtain optimal meromorphic extensions of weighted dynamical zeta functions and asymptotic counting estimates for the number of weighted closed geodesics. A meromorphic extension of the standard dynamical zeta function and the prime orbit theorem follow as corollaries. Finally, we investigate interactions between the dynamics and spectral theory of these spaces.

  5. Explicit bounds on the logarithmic derivative and the reciprocal of the Riemann zeta-function

    Trudgian, Tim


    The purpose of this article is consider $|\\zeta'(\\sigma + it)/\\zeta(\\sigma + it)|$ and $|\\zeta(\\sigma + it)|^{-1}$ when $\\sigma$ is close to unity. We prove that $|\\zeta'(\\sigma + it)/\\zeta(\\sigma + it)| \\leq 87\\log t$ and $|\\zeta(\\sigma + it)|^{-1} \\leq 6.9\\times 10^{6} \\log t$ for $\\sigma \\geq 1-1/(8 \\log t)$ and $t\\geq 45$.

  6. Seiberg Duality, Quiver Gauge Theories, and Ihara Zeta Function

    Zhou, Da; He, Yang-Hui


    We study Ihara zeta function for graphs in the context of quivers arising from gauge theories, especially under Seiberg duality transformations. The distribution of poles is studied as we proceed along the duality tree, in light of the weak and strong graph versions of the Riemann Hypothesis. As a by-product, we find a refined version of Ihara zeta function to be the generating function for the generic superpotential of the gauge theory.

  7. On the Zeta Function of a Family of Quintics

    Goutet, Philippe


    In this article, we give a proof of the link between the zeta function of two families of hypergeometric curves and the zeta function of a family of quintics that was observed numerically by Candelas, de la Ossa, and Rodriguez Villegas. The method we use is based on formulas of Koblitz and various Gauss sums identities; it does not give any geometric information on the link.

  8. Zeta potential of microfluidic substrates: 2. Data for polymers.

    Kirby, Brian J; Hasselbrink, Ernest F


    Zeta potential data are reviewed for a variety of polymeric microfluidic substrate materials. Many of these materials currently used for microchip fabrication have only recently been employed for generation of electroosmotic flow. Despite their recent history, polymeric microfluidic substrates are currently used extensively for microchip separations and other techniques, and understanding of the surface zeta potential is crucial for experimental design. This paper proposes the use of pC (the negative logarithm of the counterion concentration) as a useful normalization for the zeta potential on polymer substrates in contact with indifferent univalent counterions. Normalizing zeta by pC facilitates comparison of results from many investigators. The sparseness of available data for polymeric substrates prevents complete and rigorous justification for this normalization; however, it is consistent with double layer and adsorption theory. For buffers with indifferent univalent cations, normalization with the logarithm of the counterion concentration in general collapses data onto a single zeta/pC vs. pH curve, and (with the exception of PMMA) the repeatability of the data is quite encouraging. Normalization techniques should allow improved ability to predict zeta potential performance on microfluidic substrates and compare results observed with different parameters.

  9. Conservation of $\\zeta$ with radiative corrections from heavy field

    Tanaka, Takahiro


    In this paper, we address a possible impact of radiative corrections from a heavy scalar field $\\chi$ on the curvature perturbation $\\zeta$. Integrating out $\\chi$, we derive the effective action for $\\zeta$, which includes the loop corrections of the heavy field $\\chi$. When the mass of $\\chi$ is much larger than the Hubble scale $H$, the loop corrections of $\\chi$ only yield a local contribution in the effective action and hence the effective action simply gives an action for $\\zeta$ in a single field model, where, as is widely known, $\\zeta$ is conserved in time after the Hubble crossing time. Meanwhile, when the mass of $\\chi$ is comparable to $H$, the loop corrections of $\\chi$ can give a non-local contribution to the effective action. Because of the non-local contribution from $\\chi$, in general, $\\zeta$ may not be conserved, even if the classical background trajectory is determined only by the evolution of the inflaton. In this paper, we derive the condition that $\\zeta$ is conserved in time in the pre...

  10. A proof of the Riemann hypothesis by using the series representation of the Riemann zeta function

    Tan, Shanguang


    The Riemann hypothesis was proved in this paper. First, a curve integral of the Riemann zeta function zeta(s) was formed, which is along a horizontal line from s to 1-\\bar{s} which are two nontrivial zeros of zeta(s) and symmetric about the vertical line Re s=1/2. Next, the result of the curve integral was derived and proved equal to zero. Then, by proving a lemma of central dissymmetry of the Riemann zeta function zeta(s), two nontrivial zeros s and 1-\\bar{s} were proved being a same zero or satisfying 1-\\bar{s}=s. Hence, the nontrivial zeros of zeta(s) all have real part Re s=1/2, that is, the Riemann hypothesis was proved.

  11. Generalised root identities for zeta functions of curves over finite fields

    Stone, Richard


    We consider generalised root identities for zeta functions of curves over finite fields, \\zeta_{k}, and compare with the corresponding analysis for the Riemann zeta function. We verify numerically that, as for \\zeta, the \\zeta_{k} do satisfy the generalised root identities and we investigate these in detail for the special cases of \\mu=0,-1\\:\\&\\:-2. Unlike for \\zeta, however, we show that in the setting of zeta functions of curves over finite fields the \\mu=-2 root identity is consistent with the Riemann hypothesis (RH) proved by Weil. Comparison of this analysis with the corresponding calculations for \\zeta illuminates the fact that, even though both \\zeta and \\zeta_{k} have both Euler and Hadamard product representations, it is the detailed structure of the counting function, N(T), which drives the Cesaro computations on the root side of these identities and thereby determines the implications of the root identities for RH in each setting.

  12. On the breakdown of the curvature perturbation $\\zeta$ during reheating

    Algan, Merve Tarman; Kutluk, Emine Seyma


    It is known that in single scalar field inflationary models the standard curvature perturbation \\zeta, which is supposedly conserved at superhorizon scales, diverges during reheating at times d\\Phi/dt=0, i.e. when the time derivative of the background inflaton field vanishes. This happens because the comoving gauge \\phi=0, where \\phi\\ denotes the inflaton perturbation, breaks down when d\\Phi/dt=0. The issue is usually bypassed by averaging out the inflaton oscillations but strictly speaking the evolution of \\zeta\\ is ill posed mathematically. We solve this problem by introducing a family of smooth gauges that still eliminates the inflaton fluctuation \\phi\\ in the Hamiltonian formalism and gives a well behaved curvature perturbation \\zeta, which is now rigorously conserved at superhorizon scales. In the linearized theory, this conserved variable can be used to unambiguously propagate the inflationary perturbations from the end of inflation to subsequent epochs. We discuss the implications of our results for th...

  13. Analytic continuation of the Hurwitz Zeta Function with physical application

    Barone-Adesi, V; Adesi, Vittorio Barone; Zerbini, Sergio


    A new formula relating the analytic continuation ofthe Hurwitz zeta function to the Euler gamma function and a "Schwinger" type series is presented. In particular, the value of the derivative of the real part of the analytic continuation of the Hurwitz zeta function for even negative integers and the imaginary one for odd negative integers are explicitly given. The result can be of interest both on mathematical and physical side, because we are able to apply our new formulas in the context of the Spectral Zeta Function regularization of one-loop Quantum Field Theory, computing the exact pair production rate per space-time unit of massive Dirac particles interacting with a purely electric background field.

  14. Zeta Potential and Rheological Behavior of ULC Castables Matrix

    XU Yuanchao; WANG Zhanmin; CAO Xiying


    Effects of starting materials and four dispersants (STP, SHP, FDN and FS60) on Zeta potential and rheological behavior of alumina based ULC castables matrix were investigated. The results show that: characteristics of silica fume and alumina cements play a very important role in Zeta potential and viscosity of suspensions of the castables matrix; the dispersants STP and SHP can change Zeta potential values of the matrix suspensions remarkably; the four dispersants can effectively improve the rheological properties of matrix suspensions. For the point of lower viscosity of the matrix suspensions, the suitable additions of the three dispersants (SHP, FDN and FS60) are about 0.2% while that of STP is about 0.3%.

  15. A new generalization of the Riemann zeta function and its difference equation

    Qadir Asghar


    Full Text Available Abstract We have introduced a new generalization of the Riemann zeta function. A special case of our generalization converges locally uniformly to the Riemann zeta function in the critical strip. It approximates the trivial and non-trivial zeros of the Riemann zeta function. Some properties of the generalized Riemann zeta function are investigated. The relation between the function and the general Hurwitz zeta function is exploited to deduce new identities.

  16. Mayer Transfer Operator Approach to Selberg Zeta Function

    Momeni, Arash; Venkov, Alexei

    These notes are based on three lectures given by the second author at Copenhagen University (October 2009) and at Aarhus University, Denmark (December 2009). We mostly present here a survey of results of Dieter Mayer on relations between Selberg and Smale-Ruelle dynamical zeta functions. In a spe...... in terms of a Fredholm determinant of a classical transfer operator of the flow. The transfer operator is defined in a certain space of holomorphic functions and its matrix representation in a natural basis is given in terms of the Riemann zeta function and the Euler gamma function....

  17. From Fourier Series to Rapidly Convergent Series for Zeta(3)

    Scheufens, Ernst E


    The article presents a mathematical study which investigates the exact values of the Riemann zeta (ζ) function. It states that exact values can be determined from Fourier series for periodic versions of even power functions. It notes that using power series for logarithmic functions on this such ......The article presents a mathematical study which investigates the exact values of the Riemann zeta (ζ) function. It states that exact values can be determined from Fourier series for periodic versions of even power functions. It notes that using power series for logarithmic functions...

  18. Kabat's Surface Terms in the $\\zeta$-Function approach

    Iellici, D; Iellici, Devis; Moretti, Valter


    The thermal partition functions of photons in any covariant gauge and gravitons in the harmonic gauge, propagating in a Rindler wedge, are computed using a local zeta-function approach. The relation with the surface terms previously obtained by D. Kabat is studied. The results are discussed in relation to the quantum corrections to the black hole entropy.

  19. Kabat's Surface Terms in the Zeta-Function Approach

    Iellici, D.; Moretti, V.

    The thermal partition functions of photons in any covariant gauge and gravitons in the harmonic gauge, propagating in a Rindler wedge, are computed using a local zeta-function approach. The relation with the surface terms previously obtained by D. Kabat is studied. The results are discussed in relation to the quantum corrections to the black hole entropy.

  20. Crossing the entropy barrier of dynamical zeta functions

    Aurich, R.; Bolte, J.; Matthies, C.; Sieber, M.; Steiner, F. (Hamburg Univ. (Germany). 2. Inst. fuer Theoretische Physik)


    Dynamical zeta functions are an important tool to quantize chaotic dynamical systems. The basic quantization rules require the computation of the zeta functions on the real energy axis, where the Euler product representations running over the classical periodic orbits usually do not converge due to the existence of the so-called entropy barrier determined by the topological entropy of the classical system. We shown that the convergence properties of the dynamical zeta functions rewritten as Dirichlet series are governed not only by the well-known topological and metric entropy, but depend crucially on subtle statistical properties of the Maslow indices and of the multiplicities of the periodic orbits that are measured by a new parameter for which we introduce the notion of a third entropy. If and only if the third entropy is nonvanishing, one can cross the entropy barrier; if it exceeds a certain value, one can even compute the zeta function in the physical region by means of a convergent Dirichlet series. A simple statistical model is presented which allows to compute the third entropy. Four examples of chaotic systems are studied in detail to test the model numerically. (orig.).

  1. Some computational formulas related the Riemann zeta-function tails

    Hongmin Xu


    Full Text Available Abstract In this paper we present two computational formulae for one kind of reciprocal sums related to the Riemann zeta-function at integer points s = 4 , 5 $s=4,5$ , which answers an open problem proposed by Lin (J. Inequal. Appl. 2016:32, 2016.

  2. Zeta Regularized Product Expressions for Multiple Trigonometric Functions

    Kurokawa, Nobushige; Wakayama, Masato


    We introduce a multiple analogue of the gamma function which differs from the one defined by Barnes [B]. Using this function, we give expressions of the multiple sine and cosine functions in terms of zeta regularized products. The expression of the multiple sine function can be interpreted as a reflection formula of this new multiple analogue of the gamma function.

  3. On the zeros of the Epstein zeta function

    Mukhopadhyay, Anirban; Srinivas, Kotyada


    In this article, we count the number of consecutive zeros of the Epstein zeta-function, associated to a certain quadratic form, on the critical line with ordinates lying in $[0,T], T$ sufficiently large and which are separated apart by a given positive number $V$.

  4. Masses of the astrometric SB2 \\zeta Ori A

    Rivinius, Th; Stahl, O


    We report the first dynamic mass for an O-type supergiant, the interferometrically resolved SB2 system \\zeta Ori A (O9.5Ib+B0/1). The separation of the system excludes any previous mass-transfer, ensuring that the derived masses can be compared to single star evolutionary tracks.

  5. Dynamical zeta functions for piecewise monotone maps of the interval

    Ruelle, David


    Consider a space M, a map f:M\\to M, and a function g:M \\to {\\mathbb C}. The formal power series \\zeta (z) = \\exp \\sum ^\\infty _{m=1} \\frac {z^m}{m} \\sum _{x \\in \\mathrm {Fix}\\,f^m} \\prod ^{m-1}_{k=0} g (f^kx) yields an example of a dynamical zeta function. Such functions have unexpected analytic properties and interesting relations to the theory of dynamical systems, statistical mechanics, and the spectral theory of certain operators (transfer operators). The first part of this monograph presents a general introduction to this subject. The second part is a detailed study of the zeta functions associated with piecewise monotone maps of the interval [0,1]. In particular, Ruelle gives a proof of a generalized form of the Baladi-Keller theorem relating the poles of \\zeta (z) and the eigenvalues of the transfer operator. He also proves a theorem expressing the largest eigenvalue of the transfer operator in terms of the ergodic properties of (M,f,g).

  6. Zeta-potential of fouled thin film composite membrane

    Ikeda, K.; Hachisuka, H.; Nakamura, T. [Nitto denko Corp., Ibaraki, (Japan); Kimura, S. [Kogakuin University, Tokyo (Japan). Dept. of Environ. Chemical Engineering; Ueyama, K. [Osaka University, Osaka (Japan). Dept. of Chemical Engineering


    The surface zeta-potential of a cross-linked polyamide thin film composite reverse osmosis membrane was measured using an electrophoresis method. It was confirmed that this method could be effectively applied to analyze the fouling of such membranes. It is known that the water flux of membranes drastically decreases as a result of fouling by surfactants. Although the surfactants adsorbed on reverse osmosis membranes could not be detected by conventional methods such as SEM, EDX and FT-IR, their presence could be clarified by the profile measurements of the surface zeta-potential. The profiles of the membrane surface zeta-potentials changed to more positive values in the measured pH range as a result of fouling by cationic or amphoteric surfactants. This measuring method of surface zeta-potentials allowed us to analyze a very small amount of fouling of a thin film composite reverse osmosis membrane. This method could be used to analyze the fouled surface of the thin film composite reverse osmosis membrane which is used for production of ultrapure water and shows a remarkable decrease in flux. It also became clear that this method is easy and effective for the reverse osmosis membrane surface analysis of adsorbed materials such as surfactants. (author)

  7. On A Rapidly Converging Series For The Riemann Zeta Function

    Pichler, Alois


    To evaluate Riemann's zeta function is important for many investigations related to the area of number theory, and to have quickly converging series at hand in particular. We investigate a class of summation formulae and find, as a special case, a new proof of a rapidly converging series for the Riemann zeta function. The series converges in the entire complex plane, its rate of convergence being significantly faster than comparable representations, and so is a useful basis for evaluation algorithms. The evaluation of corresponding coefficients is not problematic, and precise convergence rates are elaborated in detail. The globally converging series obtained allow to reduce Riemann's hypothesis to similar properties on polynomials. And interestingly, Laguerre's polynomials form a kind of leitmotif through all sections.

  8. The Zeta Functions of Complexes from $\\Sp(4)$

    Fang, Yang; Wang, Chian-Jen


    Let $F$ be a non-archimedean local field with a finite residue field. To a 2-dimensional finite complex $X_\\Gamma$ arising as the quotient of the Bruhat-Tits building $X$ associated to $\\Sp_4(F)$ by a discrete torsion-free cocompact subgroup $\\Gamma$ of $\\PGSp_4(F)$, associate the zeta function $Z(X_{\\Gamma}, u)$ which counts geodesic tailless cycles contained in the 1-skeleton of $X_{\\Gamma}$. Using a representation-theoretic approach, we obtain two closed form expressions for $Z(X_{\\Gamma}, u)$ as a rational function in $u$. Equivalent statements for $X_{\\Gamma}$ being a Ramanujan complex are given in terms of vertex, edge, and chamber adjacency operators, respectively. The zeta functions of such Ramanujan complexes are distinguished by satisfying the Riemann Hypothesis.

  9. Well-rounded zeta-function of planar arithmetic lattices

    Fukshansky, Lenny


    We investigate the properties of the zeta-function of well-rounded sublattices of a fixed arithmetic lattice in the plane. In particular, we show that this function has abscissa of convergence at $s=1$ with a real pole of order 2, improving upon a recent result of S. Kuehnlein. We use this result to show that the number of well-rounded sublattices of a planar arithmetic lattice of index less or equal $N$ is $O(N \\log N)$ as $N \\to \\infty$. To obtain these results, we produce a description of integral well-rounded sublattices of a fixed planar integral well-rounded lattice and investigate convergence properties of a zeta-function of similarity classes of such lattices, building on some previous results of the author.

  10. The angular diameter and distance of the Cepheid Zeta Geminorum

    Kervella, P; Perrin, G; Schöller, M; Traub, W A; Lacasse, M D


    Cepheids are the primary distance indicators for extragalactic astronomy and therefore are of very high astrophysical interest. Unfortunately, they are rare stars, situated very far from Earth.Though they are supergiants, their typical angular diameter is only a few milliarcseconds, making them very challenging targets even for long-baseline interferometers. We report observations that were obtained in the K prime band (2-2.3 microns), on the Cepheid Zeta Geminorum with the FLUOR beam combiner, installed at the IOTA interferometer. The mean uniform disk angular diameter was measured to be 1.64 +0.14 -0.16 mas. Pulsational variations are not detected at a significant statistical level, but future observations with longer baselines should allow a much better estimation of their amplitude. The distance to Zeta Gem is evaluated using Baade-Wesselink diameter determinations, giving a distance of 502 +/- 88 pc.

  11. Lecture notes: string theory and zeta-function

    Toppan, Francesco [Centro Brasileiro de Pesquisas Fisicas (CBPF), Rio de Janeiro, RJ (Brazil). E-mail:


    These lecture notes are based on a revised and LaTexed version of the Master thesis defended at ISAS. The research part being omitted, they included a review of the bosonic closed string a la Polyakov and of the one-loop background field method of quantisation defined through the zeta-function. In an appendix some basic features of the Riemann zeta-function are also reviewed. The pedagogical aspects of the material here presented are particularly emphasized. These notes are used, together with the Scherk's article in Rev. Mod. Phys. and the first volume of the Polchinski book, for the mini-course on String Theory (16-hours of lectures) held at CBPF. In this course the Green-Schwarz-Witten two-volumes book is also used for consultative purposes. (author)

  12. Zeta-function approach to Casimir energy with singular potentials

    Khusnutdinov, N R


    In the framework of zeta-function approach the Casimir energy for three simple model system: single delta potential, step function potential and three delta potentials is analyzed. It is shown that the energy contains contributions which are peculiar to the potentials. It is suggested to renormalize the energy using the condition that the energy of infinitely separated potentials is zero which corresponds to subtraction all terms of asymptotic expansion of zeta-function. The energy obtained in this way obeys all physically reasonable conditions. It is finite in the Dirichlet limit and it may be attractive or repulsive depending on the strength of potential. The effective action is calculated and it is shown that the surface contribution appears. The renormalization of the effective action is discussed.

  13. Transient zeta-potential measurements in hydrophobic, TOPAS microfluidic substrates.

    Tandon, Vishal; Bhagavatula, Sharath K; Kirby, Brian J


    We utilize time-resolved electrokinetic measurements in order to study the electrokinetic properties of silica and TOPAS microfluidic channels as a function of the time history of the fluid-solid interface. In pressure-driven flow through TOPAS microchannels, the zeta-potential as inferred from streaming potential measurements decays exponentially by a factor of 1.5 with a characteristic decay time of 3 h after the initial formation of the fluid-solid interface. A similar exponential decay is observed immediately after water is exchanged for ethanol as the solvent in the system. In electroosmotically driven flow through TOPAS microchannels, the zeta-potential as inferred through current monitoring experiments was constant in time. No electrokinetic transients were observed in silica microchannels under these flow conditions.

  14. Consistency relations and conservation of $\\zeta$ in holographic inflation

    Garriga, Jaume


    It is well known that, in single clock inflation, the curvature perturbation $\\zeta$ is constant in time on superhorizon scales. In the standard bulk description this follows quite simply from the local conservation of the energy momentum tensor in the bulk. On the other hand, in a holographic description, the constancy of the curvature perturbation must be related to the properties of the RG flow in the boundary theory. Here, we show that, in single clock holographic inflation, the time independence of correlators of $\\zeta$ follows from the cut-off independence of correlators of the energy momentum tensor in the boundary theory, and from the so-called consistency relations for vertex functions with a soft leg.

  15. Distribution of zeta zeroes of Artin--Schreier curves

    Bucur, Alina; Feigon, Brooke; Lalin, Matilde; Sinha, Kaneenika


    We study the distribution of the zeroes of the zeta functions of the family of Artin-Schreier curves over $\\mathbb{F}_q$ when $q$ is fixed and the genus goes to infinity. We consider both the global and the mesoscopic regimes, proving that when the genus goes to infinity, the number of zeroes with angles in a prescribed interval of $[-\\pi,\\pi)$ has a standard Gaussian distribution (when properly normalized).

  16. Lowest Landau level on a cone and zeta determinants

    Klevtsov, Semyon


    We consider the integer QH state on Riemann surfaces with conical singularities, with the main objective of detecting the effect of the gravitational anomaly directly from the form of the wave function on a singular geometry. We suggest the formula expressing the normalisation factor of the holomorphic state in terms of the regularized zeta determinant on conical surfaces and check this relation for some model geometries. We also comment on possible extensions of this result to the fractional QH states.

  17. Lowest Landau level on a cone and zeta determinants

    Klevtsov, Semyon


    We consider the integer QH state on Riemann surfaces with conical singularities, with the main objective of detecting the effect of the gravitational anomaly directly from the form of the wave function on a singular geometry. We suggest the formula expressing the normalisation factor of the holomorphic state in terms of the regularized zeta determinant on conical surfaces and check this relation for some model geometries. We also comment on possible extensions of this result to the fractional QH states.

  18. On the Laurent series for the Epstein zeta function

    Joyce, G. S.


    The Epstein zeta function ζ d [ M ; s ] ≡ ∑ n ∈ Z d ‧ ( n M n T ) - s 2 , where {M} is a real symmetric and invertible d × d matrix and {n} is a d-dimensional row vector ({n}1,{n}2,\\ldots ,{n}d) with integer coordinates {n}i, is considered. (The prime on the sum indicates that the term {n}={0} should be excluded.) It is known that {\\zeta }d[{M};s] has a Laurent series expansion about the singular point s = d which can be written in the form ζ d [ M ; s ] = ∑ ν = - 1 ∞ A ν [ M ; d ] ( s - d ) ν . In this paper we shall show that the coefficient {A}ν [{M};d] can be accurately calculated using rapidly convergent series which involve the Meijer G-function. Exact formulae are also derived for {A}ν [{M};2] when M = U N ≡ 1 0 0 N , with N=1,2,\\ldots . The results for {A}0[{{U}}N;2] are then used to establish several mathematical identities involving summations of generalized Stieltjes constants. Next the Laurent series for {\\zeta }d[{{I}}d;s], where {{I}}d is the d × d unit matrix, is briefly discussed for the cases d=3,4,6 and 8. Finally, a new application of the results in lattice statistics is described.

  19. Landau-Siegel zeros and zeros of the derivative of the Riemann zeta function

    Farmer, David W


    We show that if the derivative of the Riemann zeta function has sufficiently many zeros close to the critical line, then the zeta function has many closely spaced zeros. This gives a condition on the zeros of the derivative of the zeta function which implies a lower bound of the class numbers of imaginary quadratic fields.

  20. Proof of generalized Riemann hypothesis for Dedekind zetas and Dirichlet L-functions

    Mcadrecki, Andrzej


    A short proof of the generalized Riemann hypothesis (gRH in short) for zeta functions $\\zeta_{k}$ of algebraic number fields $k$ - based on the Hecke's proof of the functional equation for $\\zeta_{k}$ and the method of the proof of the Riemann hypothesis derived in [$M_{A}$] (algebraic proof of the Riemann hypothesis) is given. The generalized Riemann hypothesis for Dirichlet L-functions is an immediately consequence of (gRH) for $\\zeta_{k}$ and suitable product formula which connects the Dedekind zetas with L-functions.

  1. Clinical efficacy and safety of biosimilar epoetin: focus on epoetin zeta

    Mikhail A


    Full Text Available Ashraf Mikhail , Christopher BrownRenal Unit, Morriston Hospital, Wales, UK Abstract: Biosimilars have been developed for several biologic therapeutic agents, including erythropoiesis-stimulating agents. Biosimilars cannot be assumed to be completely identical to the reference product. Several regulatory bodies have issued stringent guidelines to regulate the licensing of biosimilars. These guidelines, although share a unified aim of ensuring the safety and efficacy of biosimilars, show several differences. Such differences may reflect the difficulties facing regulatory bodies in defining a biosimilar, identifying sensitive means to assess equivalence in efficacy, and designing robust methodologies to monitor long-term safety. This review will discuss some of the aspects of differences in licensing requirements for biosimilars, comparing the European Medicines Agency guidelines and the American Food and Drug Administration guidelines. The pathway adopted by the manufacturer of a biosimilar (epoetin zeta to gain licensing within the European market will be assessed, analyzing its compliance with the European Medicines Agency guidelines for the approval process. Since many patients are likely to be switched from original drugs to biosimilars in future, there is a need to establish strict guidelines on interchangeability and substitution of biosimilars and original products and to make it an integral part of the pre-registration assessment of any biosimilar in future. Eventually, long-term, observational post-marketing data will provide further reassurance on safety and tolerability of biosimilars. Keywords: biosimilars, erythropoiesis-stimulating agents, epoetin zeta, biologic therapeutic agents

  2. Local zeta factors and geometries under Spec Z

    Manin, Yu I.


    The first part of this note shows that the odd-period polynomial of each Hecke cusp eigenform for the full modular group produces via the Rodriguez-Villegas transform ([1]) a polynomial satisfying the functional equation of zeta type and having non-trivial zeros only in the middle line of its critical strip. The second part discusses the Chebyshev lambda-structure of the polynomial ring as Borger's descent data to \\mathbf{F}_1 and suggests its role in a possible relation of the Γ\\mathbf{R}-factor to 'real geometry over \\mathbf{F}_1' (cf. [2]).

  3. Riemann zeta function from wave-packet dynamics

    Mack, R.; Dahl, Jens Peder; Moya-Cessa, H.


    is governed by the temperature of the thermal phase state and tau is proportional to t. We use the JWKB method to solve the inverse spectral problem for a general logarithmic energy spectrum; that is, we determine a family of potentials giving rise to such a spectrum. For large distances, all potentials...... index of JWKB. We compare and contrast exact and approximate eigenvalues of purely logarithmic potentials. Moreover, we use a numerical method to find a potential which leads to exact logarithmic eigenvalues. We discuss possible realizations of Riemann zeta wave-packet dynamics using cold atoms...

  4. Exploring the Riemann zeta function 190 years from Riemann's birth

    Nikeghbali, Ashkan; Rassias, Michael


    This book is concerned with the Riemann Zeta Function, its generalizations, and various applications to several scientific disciplines, including Analytic Number Theory, Harmonic Analysis, Complex Analysis and Probability Theory. Eminent experts in the field illustrate both old and new results towards the solution of long-standing problems and include key historical remarks. Offering a unified, self-contained treatment of broad and deep areas of research, this book will be an excellent tool for researchers and graduate students working in Mathematics, Mathematical Physics, Engineering and Cryptography.

  5. The Riemann zeta-function theory and applications

    Ivic, Aleksandar


    ""A thorough and easily accessible account.""-MathSciNet, Mathematical Reviews on the Web, American Mathematical Society. This extensive survey presents a comprehensive and coherent account of Riemann zeta-function theory and applications. Starting with elementary theory, it examines exponential integrals and exponential sums, the Voronoi summation formula, the approximate functional equation, the fourth power moment, the zero-free region, mean value estimates over short intervals, higher power moments, and omega results. Additional topics include zeros on the critical line, zero-density estim

  6. Understanding the photometric variability of {\\zeta} Ori Aa

    Buysschaert, B; Ramiaramanantsoa, T; Richardson, N D; David-Uraz, A; Moffat, A F J


    We studied the variability of the magnetic O-type supergiant $\\zeta$ Ori Aa using multi-colour BRITE photometry. We confirmed the known rotation frequency $f_{\\rm rot} = 0.15 \\pm 0.02$ c/d, and detected some of its higher harmonics, of which $4f_{\\rm rot}$ is compatible with the known DAC recurrence timescale. Thanks to simultaneous high-resolution CHIRON spectroscopy, we could identify another frequency $f_{\\rm env} = 0.10 \\pm 0.02$ c/d, caused by the circumstellar environment. Variations in the circumstellar environment are believed to cause the observed difference between the BRITE lightcurves.

  7. Sumas de Gauss y funciones zeta de hipersuperficies diagonales

    Chiara Llanos, Olmo


    A lo largo de este trabajo abordaremos algunos casos particulares de las conjeturas de Weil. En primer lugar estudiaremos la definición de función zeta de una variedad y la formulación de las conjeturas de Weil, con su respectiva motivación. Seguidamente, introduciremos toda la teoría de sumas de Gauss y de Jacobi, partiendo de la base de la teoría de caracteres de un grupo abeliano, para obtener resultados sobre el cálculo del número de puntos de ciertas variedades sobre cuerpos de caracterí...

  8. Fuzzy-PI-based centralised control of semi-isolated FP-SEPIC/ZETA BDC in a PV/battery hybrid system

    Mahendran, Venmathi; Ramabadran, Ramaprabha


    Multiport converters with centralised controller have been most commonly used in stand-alone photovoltaic (PV)/battery hybrid system to supply the load smoothly without any disturbances. This study presents the performance analysis of four-port SEPIC/ZETA bidirectional converter (FP-SEPIC/ZETA BDC) using various types of centralised control schemes like Fuzzy tuned proportional integral controller (Fuzzy-PI), fuzzy logic controller (FLC) and conventional proportional integral (PI) controller. The proposed FP-SEPIC/ZETA BDC with various control strategy is derived for simultaneous power management of a PV source using distributed maximum power point tracking (DMPPT) algorithm, a rechargeable battery, and a load by means of centralised controller. The steady state and the dynamic response of the FP-SEPIC/ZETA BDC are analysed using three different types of controllers under line and load regulation. The Fuzzy-PI-based control scheme improves the dynamic response of the system when compared with the FLC and the conventional PI controller. The power balance between the ports is achieved by pseudorandom carrier modulation scheme. The response of the FP-SEPIC/ZETA BDC is also validated experimentally using hardware prototype model of 500 W system. The effectiveness of the control strategy is validated using simulation and experimental results.

  9. Binding of phosphoinositide-specific phospholipase C-zeta (PLC-zeta) to phospholipid membranes: potential role of an unstructured cluster of basic residues.

    Nomikos, Michail; Mulgrew-Nesbitt, Anna; Pallavi, Payal; Mihalyne, Gyongyi; Zaitseva, Irina; Swann, Karl; Lai, F Anthony; Murray, Diana; McLaughlin, Stuart


    Phospholipase C-zeta (PLC-zeta) is a sperm-specific enzyme that initiates the Ca2+ oscillations in mammalian eggs that activate embryo development. It shares considerable sequence homology with PLC-delta1, but lacks the PH domain that anchors PLC-delta1 to phosphatidylinositol 4,5-bisphosphate, PIP2. Thus it is unclear how PLC-zeta interacts with membranes. The linker region between the X and Y catalytic domains of PLC-zeta, however, contains a cluster of basic residues not present in PLC-delta1. Application of electrostatic theory to a homology model of PLC-zeta suggests this basic cluster could interact with acidic lipids. We measured the binding of catalytically competent mouse PLC-zeta to phospholipid vesicles: for 2:1 phosphatidylcholine/phosphatidylserine (PC/PS) vesicles, the molar partition coefficient, K, is too weak to be of physiological significance. Incorporating 1% PIP2 into the 2:1 PC/PS vesicles increases K about 10-fold, to 5x10(3) M-1, a biologically relevant value. Expressed fragments corresponding to the PLC-zeta X-Y linker region also bind with higher affinity to polyvalent than monovalent phosphoinositides on nitrocellulose filters. A peptide corresponding to the basic cluster (charge=+7) within the linker region, PLC-zeta-(374-385), binds to PC/PS vesicles with higher affinity than PLC-zeta, but its binding is less sensitive to incorporating PIP2. The acidic residues flanking this basic cluster in PLC-zeta may account for both these phenomena. FRET experiments suggest the basic cluster could not only anchor the protein to the membrane, but also enhance the local concentration of PIP2 adjacent to the catalytic domain.

  10. Riemann Hypothesis and Random Walks: the Zeta case

    LeClair, André


    In previous work it was shown that if certain series based on sums over primes of non-principal Dirichlet characters have a conjectured random walk behavior, then the Euler product formula for its $L$-function is valid to the right of the critical line $\\Re (s) > 1/2$, and the Riemann Hypothesis for this class of $L$-functions follows. Building on this work, here we propose how to extend this line of reasoning to the Riemann zeta function and other principal Dirichlet $L$-functions. We use our results to argue that $ S_\\delta (t) \\equiv \\lim_{\\delta \\to 0^+} \\dfrac{1}{\\pi} \\arg \\zeta (\\tfrac{1}{2}+ \\delta + i t ) = O(1)$, and that it is nearly always on the principal branch. We conjecture that a 1-point correlation function of the Riemann zeros has a normal distribution. This leads to the construction of a probabilistic model for the zeros. Based on these results we describe a new algorithm for computing very high Riemann zeros as a kind of stochastic process, and we calculate the $10^{100}$-th zero to over 1...

  11. Measuring zeta potential of protein nano-particles using electroacoustics.

    Dukhin, A S; Parlia, S


    Electroacoustic spectroscopy offers a simple way for measuring the zeta potential of proteins in physiological solutions with high ionic strength. Ultrasound as a driving force does not generate the heat effects which complicate traditional electrophoretic measurements at high ionic strength. In addition, measurements can be conducted with concentrated protein dispersions without dilution, as is required by electrophoretic methods. This paper presents results for electroacoustic measurements of 5wt.% bovine serum albumin suspended in aqueous solutions. In these suspensions the proteins are not completely dissolved; they form nano-particles with a median size of about 180nm. We studied the dependence of zeta potential on ionic strength within a wide range of salt molarities, up to as high as 0.5mol/L. Dialysis was used for performing measurements at lower ionic strength range. We also conducted pH titrations of this system and titrations with Ca(2+) ions. Our results agree well with published data for samples where such data is available.

  12. Potencial zeta de sulfatos de de bario y de estroncio

    Edgar Delgado M.


    Full Text Available Por medio de la electroforesis se determinó las movilidades electroforéticas y los potenciales zeta del sulfato de bario a 25,0 °C como función de la fuerza iónica de NaCI, así como del Sulfato de estroncio en función de la fuerza iónica del cloruro de sodio y del pH. Se encontró que el amento de la fuerza iónica de NaCI causa un cambio del Potencial Zeta negativo del sulfato de estroncio a positivo con valor cero a aprox. 0,06 de fuerza iónica. El P.Z. del sulfato de estroncio es positivo a pH inferiores a aprox. 2,5 y negativo a pH superiores. El sulfato de bario presenta P.Z. negativas a fuerza iónicas de NaCI inferiores a aprox. 0.06 y PZ positivos a fuerzas iónicas mayores

  13. Measuring the zeta potential. The relationships with sandstone fineness

    de Luxán, M. P.


    Full Text Available The application of the zeta potential technique in the area of construction materials and Portland cement is quite recent. The initial research work involved the study of cement suspensions or suspensions of one of the components of cement, such as alite, tricalcium alumínate, in the presence of additives and, more specifically, superplasticizers. The studies of this sort were extended with the mixing of active additions into cement (fly ashes, etc.. The present study discusses the application of siliceous materials (sandstone as a basis of the research into the behaviour of sandstone mortars containing repair products.

    La aplicación de la técnica del potencial zeta en el campo de los materiales de construcción y del cemento portland es muy reciente. Las primeras investigaciones se refieren al estudio de suspensiones de cemento o de alguno de sus compuestos que lo forman como alita, aluminato tricálcico, en presencia de aditivos y, más concretamente, de superfluidificantes. Con la incorporación de adiciones activas al cemento (cenizas volantes,... se amplían los estudios de este tipo de cementos. En este trabajo se considera la aplicación a los materiales silíceos (arenisca como base para la investigación del comportamiento de los morteros de arenisca conteniendo productos de reparación.

  14. Investigation on Fuzzy Logic Based Centralized Control in Four-Port SEPIC/ZETA Bidirectional Converter for Photovoltaic Applications



    Full Text Available In this paper, a new four-port DC-DC converter topology is proposed to interface renewable energy sources and the load along with the energy storage device. The proposed four-port SEPIC/ZETA bidirectional converter (FP-SEPIC/ZETA BDC converter comprises an isolated output port with two unidirectional and one bidirectional input ports. This converter topology is obtained by the fusion of SEPIC/ZETA BDC and full-bridge converter. This converter topology ensures the non-reversal of output voltage hence it is preferred mostly for battery charging applications. In this work, photovoltaic (PV source is considered and the power balance in the system is achieved by means of distributed maximum power point tracking (DMPPT in the PV ports. The centralized controller is implemented using fuzzy logic controller (FLC and the performance is compared with conventional proportional integral (PI controller. The results offer useful information to obtain the desired output under line and load regulations. Experimental results are also provided to validate the simulation results.

  15. Zeta diversity as a concept and metric that unifies incidence-based biodiversity patterns.

    Hui, Cang; McGeoch, Melodie A


    Patterns in species incidence and compositional turnover are central to understanding what drives biodiversity. Here we propose zeta (ζ) diversity, the number of species shared by multiple assemblages, as a concept and metric that unifies incidence-based diversity measures, patterns, and relationships. Unlike other measures of species compositional turnover, zeta diversity partitioning quantifies the complete set of diversity components for multiple assemblages, comprehensively representing the spatial structure of multispecies distributions. To illustrate the application and ecological value of zeta diversity, we show how it scales with sample number, grain, and distance. Zeta diversity reconciles several different biodiversity patterns, including the species accumulation curve, the species-area relationship, multispecies occupancy patterns, and scaling of species endemism. Exponential and power-law forms of zeta diversity are associated with stochastic versus niche assembly processes. Zeta diversity may provide new insights on biodiversity patterns, the processes driving them, and their response to environmental change.

  16. On the local zeta functions and the b-functions of certain hyperplane arrangements

    Budur, Nero; Yuzvinsky, Sergey


    Conjectures of J. Igusa for p-adic local zeta functions and of J. Denef and F. Loeser for topological local zeta functions assert that (the real part of) the poles of these local zeta functions are roots of the Bernstein-Sato polynomials (i.e. the b-functions). We prove these conjectures for certain hyperplane arrangements, including the case of reduced hyperplane arrangements in three-dimensional affine space.

  17. Zeta function zeros, powers of primes, and quantum chaos.

    Sakhr, Jamal; Bhaduri, Rajat K; van Zyl, Brandon P


    We present a numerical study of Riemann's formula for the oscillating part of the density of the primes and their integer powers. The formula consists of an infinite series of oscillatory terms, one for each zero of the zeta function on the critical line, and was derived by Riemann in his paper on primes, assuming the Riemann hypothesis. We show that high-resolution spectral lines can be generated by the truncated series at all integer powers of primes and demonstrate explicitly that the relative line intensities are correct. We then derive a Gaussian sum rule for Riemann's formula. This is used to analyze the numerical convergence of the truncated series. The connections to quantum chaos and semiclassical physics are discussed.

  18. Hamiltonian for the Zeros of the Riemann Zeta Function.

    Bender, Carl M; Brody, Dorje C; Müller, Markus P


    A Hamiltonian operator H[over ^] is constructed with the property that if the eigenfunctions obey a suitable boundary condition, then the associated eigenvalues correspond to the nontrivial zeros of the Riemann zeta function. The classical limit of H[over ^] is 2xp, which is consistent with the Berry-Keating conjecture. While H[over ^] is not Hermitian in the conventional sense, iH[over ^] is PT symmetric with a broken PT symmetry, thus allowing for the possibility that all eigenvalues of H[over ^] are real. A heuristic analysis is presented for the construction of the metric operator to define an inner-product space, on which the Hamiltonian is Hermitian. If the analysis presented here can be made rigorous to show that H[over ^] is manifestly self-adjoint, then this implies that the Riemann hypothesis holds true.

  19. Central Binomial Sums, Multiple Clausen Values and Zeta Values

    Borwein, J M; Kamnitzer, J


    We find and prove relationships between Riemann zeta values and central binomial sums. We also investigate alternating binomial sums (also called Ap\\'ery sums). The study of non-alternating sums leads to an investigation of different types of sums which we call multiple Clausen values. The study of alternating sums leads to a tower of experimental results involving polylogarithms in the golden ratio. In the non-alternating case, there is a strong connection to polylogarithms of the sixth root of unity, encountered in the 3-loop Feynman diagrams of {\\tt hep-th/9803091} and subsequently in hep-ph/9910223, hep-ph/9910224, cond-mat/9911452 and hep-th/0004010.

  20. Where do the tedious products of zetas come from?

    Broadhurst, D J


    Lamentably, the full analytical content of the epsilon-expansion of the master two-loop two-point function, with arbitrary self-energy insertions in 4-2epsilon dimensions, is still unknown. Here we show that multiple zeta values (MZVs) of weights up to 12 suffice through O(epsilon^9). Products of primitive MZVs are generated by a processes of "pseudo-exponentiation"" whose combinatorics faithfully accord with expectations based on Kreimer's modified shuffle product and on the Drinfeld-Deligne conjecture. The existence of such a mechanism, relating thousands of complicated rational numbers, enables us to identify precise and simple combinations of MZVs specific to quantum field theories in even numbers of spacetime dimensions.

  1. Zeta Potential of Modified Multi-walled Carbon Nanotubes in Presence of poly (vinyl alcohol) Hydrogel

    de la Cruz, EF; Zheng, Y.; Torres, E; Li, W.; Song, W; Burugapalli, K


    The main objective of this study is investigate the behavior of the Zeta Potential of the MWCNT modified with SDS(Sodium Dodecyl Sulfate) and CTAB(Cetyl Tetraethyl Ammonium Bromide) in presence of PVA. Full hydrolyzed PVA was used. As a result, adding PVA in the CNT solution led to decrease the Zeta Potential. The Zeta Potential of suspended colloid varied from 42.00mV to 6.48mV and -45.00mV to -6.4mV at 1.5% concentration of PVA; according with the changing pH, the Zeta Potential dropped to ...

  2. Zeta Function Expression of Spin Partition Functions on Thermal AdS3

    Floyd L.Williams


    Full Text Available We find a Selberg zeta function expression of certain one-loop spin partition functions on three-dimensional thermal anti-de Sitter space. Of particular interest is the partition function of higher spin fermionic particles. We also set up, in the presence of spin, a Patterson-type formula involving the logarithmic derivative of zeta.

  3. An elementary and real approach to values of the Riemann zeta function

    Bagdasaryan, A. G.


    An elementary approach for computing the values at negative integers of the Riemann zeta function is presented. The approach is based on a new method for ordering the integers. We show that the values of the Riemann zeta function can be computed, without using the theory of analytic continuation and any knowledge of functions of complex variable.

  4. Random matrix theory and discrete moments of the Riemann zeta function

    Hughes, C P [Raymond and Beverly Sackler School of Mathematical Sciences, Tel Aviv University, Tel Aviv 69978 (Israel)


    We calculate the discrete moments of the characteristic polynomial of a random unitary matrix, evaluated a small distance away from an eigenangle. Such results allow us to make conjectures about similar moments for the Riemann zeta function, and provide a uniform approach to understanding moments of the zeta function and its derivative.

  5. Multi-loop zeta function regularization and spectral cutoff in curved spacetime

    Bilal, Adel, E-mail: [Centre National de la Recherche Scientifique, Laboratoire de Physique Théorique de l' École Normale Supérieure, 24 rue Lhomond, F-75231 Paris Cedex 05 (France); Ferrari, Frank, E-mail: [Service de Physique Théorique et Mathématique, Université Libre de Bruxelles and International Solvay Institutes, Campus de la Plaine, CP 231, B-1050 Bruxelles (Belgium)


    We emphasize the close relationship between zeta function methods and arbitrary spectral cutoff regularizations in curved spacetime. This yields, on the one hand, a physically sound and mathematically rigorous justification of the standard zeta function regularization at one loop and, on the other hand, a natural generalization of this method to higher loops. In particular, to any Feynman diagram is associated a generalized meromorphic zeta function. For the one-loop vacuum diagram, it is directly related to the usual spectral zeta function. To any loop order, the renormalized amplitudes can be read off from the pole structure of the generalized zeta functions. We focus on scalar field theories and illustrate the general formalism by explicit calculations at one-loop and two-loop orders, including a two-loop evaluation of the conformal anomaly.

  6. Influence of surface conductivity on the apparent zeta potential of calcite

    Li, Shuai; Heberling, Frank; Devau, Nicolas; Jougnot, Damien; Chiaberge, Christophe


    Zeta potential is a physicochemical parameter of particular importance in describing the surface electrical properties of charged porous media. However, the zeta potential of calcite is still poorly known because of the difficulty to interpret streaming potential experiments. The Helmholtz-Smoluchowski (HS) equation is widely used to estimate the apparent zeta potential from these experiments. However, this equation neglects the influence of surface conductivity on streaming potential. We present streaming potential and electrical conductivity measurements on a calcite powder in contact with an aqueous NaCl electrolyte. Our streaming potential model corrects the apparent zeta potential of calcite by accounting for the influence of surface conductivity and flow regime. We show that the HS equation seriously underestimates the zeta potential of calcite, particularly when the electrolyte is diluted (ionic strength < 0.01 M) because of calcite surface conductivity. The basic Stern model successfully predicted ...

  7. Intermediate- and High-Velocity Ionized Gas toward zeta Orionis

    Welty, D E; Raymond, J C; Mallouris, C; York, D G


    We combine UV spectra obtained with the HST/GHRS echelle, IMAPS, and Copernicus to study the abundances and physical conditions in the predominantly ionized gas seen at high (-105 to -65 km/s) and intermediate velocities (-60 to -10 km/s) toward zeta Ori. We have high resolution (FWHM ~ 3.3-4.5 km/s) and/or high S/N spectra for at least two significant ions of C, N, Al, Si, S, and Fe -- enabling accurate estimates for both the total N(H II) and the elemental depletions. C, N, and S have essentially solar relative abundances; Al, Si, and Fe appear to be depleted by about 0.8, 0.3-0.4, and 0.95 dex, respectively. While various ion ratios would be consistent with collisional ionization equilibrium (CIE) for T ~ 25,000-80,000 K, the widths of individual high-velocity absorption components indicate that T ~ 9000 K -- so the gas is not in CIE. Analysis of the C II fine-structure excitation equilibrium yields estimated densities (n_e ~ n_H ~ 0.1-0.2 cm^{-3}), thermal pressures (2 n_H T ~ 2000-4000 cm^{-3}K), and thi...

  8. Zeta potentials in the flotation of oxide and silicate minerals.

    Fuerstenau, D W; Pradip


    Adsorption of collectors and modifying reagents in the flotation of oxide and silicate minerals is controlled by the electrical double layer at the mineral-water interface. In systems where the collector is physically adsorbed, flotation with anionic or cationic collectors depends on the mineral surface being charged oppositely. Adjusting the pH of the system can enhance or prevent the flotation of a mineral. Thus, the point of zero charge (PZC) of the mineral is the most important property of a mineral in such systems. The length of the hydrocarbon chain of the collector is important because of chain-chain association enhances the adsorption once the surfactant ions aggregate to form hemimicelles at the surface. Strongly chemisorbing collectors are able to induce flotation even when collector and the mineral surface are charged similarly, but raising the pH sufficiently above the PZC can repel chemisorbing collectors from the mineral surface. Zeta potentials can be used to delineate interfacial phenomena in these various systems.

  9. Advances in random matrix theory, zeta functions, and sphere packing.

    Hales, T C; Sarnak, P; Pugh, M C


    Over four hundred years ago, Sir Walter Raleigh asked his mathematical assistant to find formulas for the number of cannonballs in regularly stacked piles. These investigations aroused the curiosity of the astronomer Johannes Kepler and led to a problem that has gone centuries without a solution: why is the familiar cannonball stack the most efficient arrangement possible? Here we discuss the solution that Hales found in 1998. Almost every part of the 282-page proof relies on long computer verifications. Random matrix theory was developed by physicists to describe the spectra of complex nuclei. In particular, the statistical fluctuations of the eigenvalues ("the energy levels") follow certain universal laws based on symmetry types. We describe these and then discuss the remarkable appearance of these laws for zeros of the Riemann zeta function (which is the generating function for prime numbers and is the last special function from the last century that is not understood today.) Explaining this phenomenon is a central problem. These topics are distinct, so we present them separately with their own introductory remarks.

  10. Standard Model with extra dimensions and its zeta function regularization

    García-Jiménez, I; Martínez-Pascual, E; Nápoles-Cañedo, G I; Novales-Sánchez, H; Toscano, J J


    We start from a field theory governed by the extra-dimensional $ISO(1,3+n)$ Poincar\\'e group and by the extended SM gauge group, $G({\\cal M}^{4+n})$. Then we construct an effective field theory whose symmetry groups are $ISO(1,3)$ and $G({\\cal M}^{4})$. The transition is carried out via two canonical transformations: a map that preserves, but it hides, the $SO(1,3+n)$ symmetry; and a transformation, given by Fourier series, that explicitly breaks $ISO(1,3+n)$ into $ISO(1,3)$, but conserves and hides the gauge symmetry $G({\\cal M}^{4+n})$, which manifests through nonstandard gauge transformations. From the 4-dimensional perspective, a particle that propagates in compact extra dimensions unfolds into a family of fields that reduces to the SM field if the size of the compact manifold is negligible. We include a full catalogue of Lagrangian terms that can be used to derive Feynman rules. The divergent character of the theory at one-loop is studied. A regularization scheme, based on the Epstein zeta function (EZF)...

  11. DNA polymerase zeta (polζ) in higher eukaryotes

    Gregory N Gan; John P Wittschieben; Birgitte φ Wittschieben; Richard D Wood


    Most current knowledge about DNA polymerase zeta (pol ζ) comes from studies of the enzyme in the budding yeast Saccharomyces cerevisiae, where polζ consists of a complex of the catalytic subunit Rev3 with Rev7, which associates with Rev1. Most spontaneous and induced mutagenesis in yeast is dependent on these gene products, and yeast pol can mediate translesion DNA synthesis past some adducts in DNA templates. Study of the homologous gene products in higher eukaryotes is in a relatively early stage, but additional functions for the eukaryotic proteins are already appar-ent. Suppression of vertebrate REV3L function not only reduces induced point mutagenesis but also causes larger-scale genuine instability by raising the frequency of spontaneous chromosome translocations. Disruption of Rev3L function is tolerated in Drosophila, Arabidopsis, and in vertebrate cell lines under some conditions, but is incompatible with mouse embryonic development. Functions for REV3L and REV7(MAD2B) in higher eukaryotes have been suggested not only in translesion DNA synthesis but also in some forms of homologous recombination, repair ofinterstrand DNA erosslinks, somatic hypermutation of immunoglobulin genes and cell-cycle control. This review discusses recent devel-opments in these areas.

  12. On the minima and convexity of Epstein zeta function

    Lim, S. C.; Teo, L. P.


    Let Zn(s ;a1,…,an) be the Epstein zeta function defined as the meromorphic continuation of the function ∑k εZn{0}(∑i =1n[aiki]2)-s, Re s>n/2 to the complex plane. We show that for fixed s ≠n/2, the function Zn(s ;a1,…,an) as a function of (a1,…,an)ε(R+)n with fixed ∏i =1nai has a unique minimum at the point a1=⋯=an. When ∑i =1nci is fixed, the function (c1,…,cn)↦Zn(s ;ec1,…,ecn) can be shown to be a convex function of any (n -1) of the variables {c1,…,cn}. These results are then applied to the study of the sign of Zn(s ;a1,…,an) when s is in the critical range (0,n/2). It is shown that when 1≤n≤9, Zn(s ;a1,…,an) as a function of (a1,…,an)ε(R+)n can be both positive and negative for every s ε(0,n/2). When n ≥10, there are some open subsets In,+ of s ε(0,n/2), where Zn(s ;a1,…,an) is positive for all (a1,…,an)ε(R+)n. By regarding Zn(s ;a1,…,an) as a function of s, we find that when n ≥10, the generalized Riemann hypothesis is false for all (a1,…,an).

  13. Influence of surface conductivity on the apparent zeta potential of calcite.

    Li, Shuai; Leroy, Philippe; Heberling, Frank; Devau, Nicolas; Jougnot, Damien; Chiaberge, Christophe


    Zeta potential is a physicochemical parameter of particular importance in describing the surface electrical properties of charged porous media. However, the zeta potential of calcite is still poorly known because of the difficulty to interpret streaming potential experiments. The Helmholtz-Smoluchowski (HS) equation is widely used to estimate the apparent zeta potential from these experiments. However, this equation neglects the influence of surface conductivity on streaming potential. We present streaming potential and electrical conductivity measurements on a calcite powder in contact with an aqueous NaCl electrolyte. Our streaming potential model corrects the apparent zeta potential of calcite by accounting for the influence of surface conductivity and flow regime. We show that the HS equation seriously underestimates the zeta potential of calcite, particularly when the electrolyte is diluted (ionic strength ⩽ 0.01 M) because of calcite surface conductivity. The basic Stern model successfully predicted the corrected zeta potential by assuming that the zeta potential is located at the outer Helmholtz plane, i.e. without considering a stagnant diffuse layer at the calcite-water interface. The surface conductivity of calcite crystals was inferred from electrical conductivity measurements and computed using our basic Stern model. Surface conductivity was also successfully predicted by our surface complexation model.

  14. Determinations of rational Dedekind-$\\zeta$ invariants of hyperbolic manifolds and Feynman knots and links

    Borwein, J M


    We identify 998 closed hyperbolic 3-manifolds whose volumes are rationally related to Dedekind zeta values, with coprime integers $a$ and $b$ giving $a/b vol(M)=(-D)^{3/2}/(2\\pi)^{2n-4} (\\zeta_K(2))/(2\\zeta(2))$ for a manifold M whose invariant trace field $K$ has a single complex place, discriminant $D$, degree $n$, and Dedekind zeta value $\\zeta_K(2)$. The largest numerator of the 998 invariants of Hodgson-Weeks manifolds is, astoundingly, $a=2^4\\times23\\times37\\times691 =9,408,656$; the largest denominator is merely b=9. We also study the rational invariant a/b for single-complex-place cusped manifolds, complementary to knots and links, both within and beyond the Hildebrand-Weeks census. Within the censi, we identify 152 distinct Dedekind zetas rationally related to volumes. Moreover, 91 census manifolds have volumes reducible to pairs of these zeta values. Motivated by studies of Feynman diagrams, we find a 10-component 24-crossing link in the case n=2 and D=-20. It is one of 5 alternating platonic links,...

  15. A trio of Bernoulli relations, their implications for the Ramanujan polynomials and the zeta constants

    Lettington, Matthew C


    We study the interplay between recurrences for zeta related functions at integer values, `Minor Corner Lattice' Toeplitz determinants and integer composition based sums. Our investigations touch on functional identities due to Ramanujan and Grosswald, the transcendence of the zeta function at odd integer values, the Li Criterion for the Riemann Hypothesis and pseudo-characteristic polynomials for zeta related functions. We begin with a result of Lettington's and some seemingly new Bernoulli relations, which we use to obtain a generalised Ramanujan polynomial and properties thereof.

  16. Jacob's ladders and some unbounded decomposition of the $\\zeta$-oscillating systems on products of other $\\zeta$-oscillating systems as an $\\zeta$-analogue of the prime decomposition

    Moser, Jan


    In this paper we introduce new class of multiplicative interactions of the $\\zeta$-oscillating systems generated by a subset of power functions. The main result obtained expresses an analogue of prime decomposition (without the property of uniqueness). Dedicated to recalling of Nicola Tesla's oscillators

  17. Granulometric structure, zeta potential and differential scanning calorimetry of native starch powders from Dioscorea spp.

    Gildas K. Gbassi


    Full Text Available Starch powders from two cultivars of Dioscorea rotundata (DR were analysed on the physicochemical aspect. Granulometric structure, zeta potential and differential scanning calorimetry of starch powders showed the following properties. Various shapes with predominance of granule ranging from 10 to 40 μm were noted. The zeta potential of DR went from positive values to negative values as the pH was increasing from 2 to 8. From pH 2 to 4, the zeta potential was positive. A significant difference was obtained between each value (p > 0.05. The zeta potential took a negative value from pH 5 and above. The results of thermal analysis show that starches start swelling at 68.91.5 C. Enthalpy of gelatinization was about 15 J.g-1 .

  18. On the sign of the real part of the Riemann zeta-function

    de Reyna, Juan Arias; van de Lune, Jan


    We consider the distribution of $\\arg\\zeta(\\sigma+it)$ on fixed lines $\\sigma > \\frac12$, and in particular the density \\[d(\\sigma) = \\lim_{T \\rightarrow +\\infty} \\frac{1}{2T} |\\{t \\in [-T,+T]: |\\arg\\zeta(\\sigma+it)| > \\pi/2\\}|\\,,\\] and the closely related density \\[d_{-}(\\sigma) = \\lim_{T \\rightarrow +\\infty} \\frac{1}{2T} |\\{t \\in [-T,+T]: \\Re\\zeta(\\sigma+it) < 0\\}|\\,.\\] Using classical results of Bohr and Jessen, we obtain an explicit expression for the characteristic function $\\psi_\\sigma(x)$ associated with $\\arg\\zeta(\\sigma+it)$. We give explicit expressions for $d(\\sigma)$ and $d_{-}(\\sigma)$ in terms of $\\psi_\\sigma(x)$. Finally, we give a practical algorithm for evaluating these expressions to obtain accurate numerical values of $d(\\sigma)$ and $d_{-}(\\sigma)$.

  19. Random matrix theory and the zeros of {zeta}'(s)

    Mezzadri, Francesco [School of Mathematics, University of Bristol, University Walk, Bristol, BS8 1TW, UK (United Kingdom)


    We study the density of the roots of the derivative of the characteristic polynomial Z(U, z) of an N x N random unitary matrix with distribution given by Haar measure on the unitary group. Based on previous random matrix theory models of the Riemann zeta function {zeta}(s), this is expected to be an accurate description for the horizontal distribution of the zeros of {zeta}'(s) to the right of the critical line. We show that as N {yields} {infinity} the fraction of the roots of Z'(U, z) that lie in the region 1 - x/(N - 1) {<=} vertical bar z vertical bar < 1 tends to a limit function. We derive asymptotic expressions for this function in the limits x {yields} {infinity} and x {yields} 0 and compare them with numerical experiments.

  20. First detection of a magnetic field in the fast rotating runaway Oe star zeta Ophiuchi

    Hubrig, S; Schoeller, M


    The star zeta Ophiuchi is one of the brightest massive stars in the northern hemisphere and was intensively studied in various wavelength domains. The currently available observational material suggests that certain observed phenomena are related to the presence of a magnetic field. We acquired spectropolarimetric observations of zeta Oph with FORS1 mounted on the 8-m Kueyen telescope of the VLT to investigate if a magnetic field is indeed present in this star. Using all available absorption lines, we detect a mean longitudinal magnetic field B_z_all = 141+-45G, confirming the magnetic nature of this star. We review the X-ray properties of zeta Oph with the aim to understand whether the X-ray emission of zeta Oph is dominated by magnetic or by wind instability processes.

  1. Isotypic Decomposition of the Cohomology and Factorization of the Zeta Functions of Dwork Hypersurfaces

    Goutet, Philippe


    The aim of this article is to illustrate, on the example of Dwork hypersurfaces, how the study of the representation of a finite group of automorphisms of a hypersurface in its etale cohomology allows to factor its zeta function.

  2. Physical properties of nanofluid suspension of ferromagnetic graphite with high Zeta potential

    Souza, N. S.; Rodrigues, A. D.; Cardoso, C. A.; Pardo, H.; Faccio, R.; Mombru, A. W.; Galzerani, J. C.; de Lima, O. F.; Sergeenkov, S.; Araujo-Moreira, F. M.


    We report on the magnetic properties and stability of nanofluid ferromagnetic graphite (NFMG) studied through the measurements of its magnetization hysteresis curves, Raman spectrum and the so-called Zeta potential. The obtained results suggest a robust ferromagnetic behavior of NFMG even at room temperature along with a good stability of the dispersed solution (with Zeta potential around 41.3 mV) and a good reactivity between magnetic graphite and CTAB type cationic surfactant.

  3. Seiberg duality, quiver gauge theories, and Ihara’s zeta function

    Zhou, Da; Xiao, Yan; He, Yang-Hui


    We study Ihara’s zeta function for graphs in the context of quivers arising from gauge theories, especially under Seiberg duality transformations. The distribution of poles is studied as we proceed along the duality tree, in light of the weak and strong graph versions of the Riemann Hypothesis. As a by-product, we find a refined version of Ihara’s zeta function to be the generating function for the generic superpotential of the gauge theory.

  4. VizieR Online Data Catalog: Chemical abundances of zeta Reticuly (Adibekyan+, 2016)

    Adibekyan, V.; Delgado-Mena, E.; Figueira, P.; Sousa, S. G.; Santos, N. C.; Faria, J. P.; Gonzalez Hernandez, J. I.; Israelian, G.; Harutyunyan, G.; Suarez-Andres, L.; Hakobyan, A. A.


    The file table1.dat lists stellar parameters, S/N, and observation dates of zeta1 Ret and zeta2 Ret derived from individual and combined spectra The file ew.dat lists the equivalent widths (EW) of all the spectral lines. The file s_lines.dat lists the lines that were used in this study. The file abund.dat lists the derived abundances of the elements for each star and spectra. (4 data files).

  5. The weak magnetic field of the O9.7 supergiant zeta Orionis A

    Bouret, J -C; Martins, F; Escolano, C; Marcolino, W; Lanz, T; Howarth, Ian


    We report here the detection of a weak magnetic field of 50 - 100 G on the O9.7 supergiant zeta Ori A, using spectropolarimetric observations obtained with NARVAL at the 2m Telescope Bernard Lyot atop Pic du Midi (France). zeta Ori A is the third O star known to host a magnetic field (along with theta^1 Ori C and HD 191612), and the first detection on a 'normal' rapidly-rotating O star. The magnetic field of zeta Ori A is the weakest magnetic field ever detected on a massive star. The measured field is lower than the thermal equipartition limit (about 100 G). By fitting NLTE model atmospheres to our spectra, we determined that zeta Ori A is a 40 Msun star with a radius of 25 Rsun and an age of about 5 - 6 Myr, showing no surface nitrogen enhancement and losing mass at a rate of about 2x10^(-6) Msol/yr. The magnetic topology of zeta Ori A is apparently more complex than a dipole and involves two main magnetic polarities located on both sides of the same hemisphere; our data also suggest that zeta Ori A rotates...

  6. Optical tweezers as a new biomedical tool to measure zeta potential of stored red blood cells.

    Diego C N Silva

    Full Text Available During storage, red blood cells (RBCs for transfusion purposes suffer progressive deterioration. Sialylated glycoproteins of the RBC membrane are responsible for a negatively charged surface which creates a repulsive electrical zeta potential. These charges help prevent the interaction between RBCs and other cells, and especially among each RBCs. Reports in the literature have stated that RBCs sialylated glycoproteins can be sensitive to enzymes released by leukocyte degranulation. Thus, the aim of this study was, by using an optical tweezers as a biomedical tool, to measure the zeta potential in standard RBCs units and in leukocyte reduced RBC units (collected in CPD-SAGM during storage. Optical tweezers is a sensitive tool that uses light for measuring cell biophysical properties which are important for clinical and research purposes. This is the first study to analyze RBCs membrane charges during storage. In addition, we herein also measured the elasticity of RBCs also collected in CPD-SAGM. In conclusion, the zeta potential decreased 42% and cells were 134% less deformable at the end of storage. The zeta potential from leukodepleted units had a similar profile when compared to units stored without leukoreduction, indicating that leukocyte lyses were not responsible for the zeta potential decay. Flow cytometry measurements of reactive oxygen species suggested that this decay is due to membrane oxidative damages. These results show that measurements of zeta potentials provide new insights about RBCs storage lesion for transfusion purposes.

  7. Improving the Efficiency of a Coagulation-Flocculation Wastewater Treatment of the Semiconductor Industry through Zeta Potential Measurements

    Eduardo Alberto López-Maldonado


    Full Text Available Efficiency of coagulation-flocculation process used for semiconductor wastewater treatment was improved by selecting suitable conditions (pH, polyelectrolyte type, and concentration through zeta potential measurements. Under this scenario the zeta potential, ζ, is the right parameter that allows studying and predicting the interactions at the molecular level between the contaminants in the wastewater and polyelectrolytes used for coagulation-flocculation. Additionally, this parameter is a key factor for assessing the efficiency of coagulation-flocculation processes based on the optimum dosages and windows for polyelectrolytes coagulation-flocculation effectiveness. In this paper, strategic pH variations allowed the prediction of the dosage of polyelectrolyte on wastewater from real electroplating baths, including the isoelectric point (IEP of the dispersions of water and commercial polyelectrolytes used in typical semiconductor industries. The results showed that there is a difference between polyelectrolyte demand required for the removal of suspended solids, turbidity, and organic matter from wastewater (23.4 mg/L and 67 mg/L, resp.. It was also concluded that the dose of polyelectrolytes and coagulation-flocculation window to achieve compliance with national and international regulations as EPA in USA and SEMARNAT in Mexico is influenced by the physicochemical characteristics of the dispersions and treatment conditions (pH and polyelectrolyte dosing strategy.

  8. Proof of Analytic Extension Theorem for Zeta Function Using Abel Transformation and Euler Product

    Mbaitiga Zacharie


    Full Text Available Problem statement: In the prime number the Riemann zeta function is unquestionable and undisputable one of the most important questions in mathematics whose many researchers are still trying to find answer to some unsolved problems such as Riemann Hypothesis. In this study we proposed a new method that proves the analytic extension theorem for zeta function. Approach: Abel transformation was used to prove that the extension theorem is true for the real part of the complex variable that is strictly greater than one and consequently provides the required analytic extension of the zeta function to the real part greater than zero and Euler product was used to prove the real part of the complex that are less than zero and greater or equal to one. Results: From this proposed study we noted that the real values of the complex variable are lying between zero and one which may help to understand the relation between zeta function and its properties and consequently can pay the way to solve some complex arithmetic problems including the Riemann Hypothesis. Conclusion: The combination of Abel transformation and Euler product is a powerful tool for proving theorems and functions related to Zeta function including other subjects such as radio atmospheric occultation.

  9. Riemann Zeta Zeros and Prime Number Spectra in Quantum Field Theory

    Menezes, G.; Svaiter, B. F.; Svaiter, N. F.


    The Riemann hypothesis states that all nontrivial zeros of the zeta function lie in the critical line Re(s) = 1/2. Hilbert and Pólya suggested that one possible way to prove the Riemann hypothesis is to interpret the nontrivial zeros in the light of spectral theory. Using the construction of the so-called super-zeta functions or secondary zeta functions built over the Riemann nontrivial zeros and the regularity property of one of this function at the origin, we show that it is possible to extend the Hilbert-Pólya conjecture to systems with countably infinite number of degrees of freedom. The sequence of the nontrivial zeros of the Riemann zeta function can be interpreted as the spectrum of a self-adjoint operator of some hypothetical system described by the functional approach to quantum field theory. However, if one considers the same situation with numerical sequences whose asymptotic distributions are not "far away" from the asymptotic distribution of prime numbers, the associated functional integral cannot be constructed. Finally, we discuss possible relations between the asymptotic behavior of a sequence and the analytic domain of the associated zeta function.

  10. Zeta functions and regularized determinants related to the Selberg trace formula

    Momeni, Arash; Venkov, Alexei

    For a general Fuchsian group of the first kind with an arbitrary unitary representation we define the zeta functions related to the contributions of the identity, hyperbolic, elliptic and parabolic conjugacy classes in Selberg's trace formula. We present Selberg's zeta function in terms of a regu......For a general Fuchsian group of the first kind with an arbitrary unitary representation we define the zeta functions related to the contributions of the identity, hyperbolic, elliptic and parabolic conjugacy classes in Selberg's trace formula. We present Selberg's zeta function in terms...... determinants of one dimensional Schroedinger operator for harmonic oscillator. We decompose the determinant of the automorphic Laplacian into a product of the determinants where each factor is a determinant representation of a zeta function related to Selberg's trace formula. Then we derive an identity...... connecting the determinants of the automorphic Laplacians on different Riemannian surfaces related to the arithmetical groups. Finally, by using the Jacquet-Langlands correspondence we connect the determinant of the automorphic Laplacian for the unit group of quaternions to the product of the determinants...

  11. Zeta potential of selected bacteria in drinking water when dead, starved, or exposed to minimal and rich culture media.

    Soni, Kamlesh A; Balasubramanian, Ashwin K; Beskok, Ali; Pillai, Suresh D


    The zeta potentials of E. coli, GFP (green fluorescence protein)-labeled E. coli, Salmonella Newport, and Pseudomonas sp. in different states (nutrient-starved and dead) and grown in rich and minimal media were measured. Capillary electrophoresis experiments were conducted to measure the zeta potential of the different cells suspended in a drinking water sample. Salmonella Newport strain showed a lower zeta potential compared to E. coli, GFP-labeled E. coli, and Pseudomonas sp. Starved E. coli cells had a lower zeta potential compared to E. coli cells grown under rich media conditions. Salmonella Newport cells grown in minimal media also had a lower zeta potential compared to rich, starved, and dead cells. The different bacterial cell types exhibited differences in size as well. These results suggest that when bacterial cells are present in drinking water they can exhibit significant heterogeneity in the size and zeta potential, depending on their physiological state.

  12. Physical properties of nanofluid suspension of ferromagnetic graphite with high Zeta potential

    Souza, N.S. [Materials and Devices Group, Department of Physics, Universidade Federal de São Carlos, 13565-905 São Carlos, SP (Brazil); Rodrigues, A.D. [Raman Spectroscopy in Nanostructured Materials, Department of Physics, Universidade Federal de São Carlos, 13565-905 São Carlos, SP (Brazil); Cardoso, C.A. [Materials and Devices Group, Department of Physics, Universidade Federal de São Carlos, 13565-905 São Carlos, SP (Brazil); Pardo, H.; Faccio, R.; Mombru, A.W. [Crystallography, Solid State and Materials Laboratory (Cryssmat-Lab), DEQUIFIM, Facultad de Química, Universidad de la República, P.O. Box 1157, CP 11800, Montevideo (Uruguay); Galzerani, J.C. [Raman Spectroscopy in Nanostructured Materials, Department of Physics, Universidade Federal de São Carlos, 13565-905 São Carlos, SP (Brazil); Lima, O.F. de [Instituto de Física “Gleb Wataghin”, UNICAMP, 13083-970 Campinas, SP (Brazil); Sergeenkov, S., E-mail: sergei@df.ufscar [Materials and Devices Group, Department of Physics, Universidade Federal de São Carlos, 13565-905 São Carlos, SP (Brazil); Araujo-Moreira, F.M. [Materials and Devices Group, Department of Physics, Universidade Federal de São Carlos, 13565-905 São Carlos, SP (Brazil)


    We report on the magnetic properties and stability of nanofluid ferromagnetic graphite (NFMG) studied through the measurements of its magnetization hysteresis curves, Raman spectrum and the so-called Zeta potential. The obtained results suggest a robust ferromagnetic behavior of NFMG even at room temperature along with a good stability of the dispersed solution (with Zeta potential around 41.3 mV) and a good reactivity between magnetic graphite and CTAB type cationic surfactant. -- Highlights: ► Magnetic properties and stability of nanofluid ferromagnetic graphite (NFMG) are studied. ► The magnetization hysteresis curves suggest a robust ferromagnetic behavior of NFMG even at room temperature. ► NFMG is found to have Zeta potential around 41.3 mV indicating a good stability of the dispersed solution.

  13. Zeta-Functions for Families of Calabi--Yau n-folds with Singularities

    Frühbis-Krüger, Anne


    We consider families of Calabi-Yau n-folds containing singular fibres and study relations between the occurring singularity structure and the decomposition of the local Weil zeta-function. For 1-parameter families, this provides new insights into the combinatorial structure of the strong equivalence classes arising in the Candelas - de la Ossa - Rodrigues-Villegas approach for computing the zeta-function. This can also be extended to families with more parameters as is explored in several examples, where the singularity analysis provides correct predictions for the changes of degree in the decomposition of the zeta-function when passing to singular fibres. These observations provide first evidence in higher dimensions for Lauder's conjectured analogue of the Clemens-Schmid exact sequence.

  14. Uniform asymptotics for the full moment conjecture of the Riemann zeta function

    Hiary, Ghaith A


    Conrey, Farmer, Keating, Rubinstein, and Snaith recently conjectured formulas for the full asymptotics of the moments of $L$-functions. In the case of the Riemann zeta function, their conjecture states that the $2k$-th absolute moment of zeta on the critical line is asymptotically given by a certain $2k$-fold residue integral. This residue integral can be expressed as a polynomial of degree $k^2$, whose coefficients are given in exact form by elaborate and complicated formulas. In this article, uniform asymptotics for roughly the first $k$ coefficients of the moment polynomial are derived. Numerical data to support our asymptotic formula are presented. An application to bounding the maximal size of the zeta function is considered.

  15. Selberg zeta functions and transfer operators an experimental approach to singular perturbations

    Fraczek, Markus Szymon


    This book presents a method for evaluating Selberg zeta functions via transfer operators for the full modular group and its congruence subgroups with characters. Studying zeros of Selberg zeta functions for character deformations allows us to access the discrete spectra and resonances of hyperbolic Laplacians under both singular and non-singular perturbations. Areas in which the theory has not yet been sufficiently developed, such as the spectral theory of transfer operators or the singular perturbation theory of hyperbolic Laplacians, will profit from the numerical experiments discussed in this book. Detailed descriptions of numerical approaches to the spectra and eigenfunctions of transfer operators and to computations of Selberg zeta functions will be of value to researchers active in analysis, while those researchers focusing more on numerical aspects will benefit from discussions of the analytic theory, in particular those concerning the transfer operator method and the spectral theory of hyperbolic spac...


    Nishi K. Bhardwaj; Sanjay Kumar; Pramod K. Bajpai


    Thc zeta potential and cationic demands of various kraft pulps - unbleached, bleached and beaten to different freeness levels were determined in the laboratory. The zeta potential of pulp where the final stage was a lignin degrading and dissolving treatment such as alkaline extraction or hypochlorite was lower than the unbleached pulp. The cationic demand of unbleached pulp was higher compared to that of bleached pulp. Beaten pulps showed larger cationic demands than the unbeaten pulps, which can be attributed to the larger specific surface area of pulp components. Fibres become increasingly electronegative when beaten and therefore cationic demand of pulps increases with beating. When the zeta potential method is used with furnishes of different freeness,the linear relationship is observed. However, the slope is decreasing with the decrease in freeness of the pulp.

  17. Zeta potential change of Neuro-2a tumor cells after exposure to alumina nanoparticles

    Kazantsev, Sergey O.; Fomenko, Alla N.; Korovin, Matvey S.


    In recent years, researches have paid much attention to the physical, chemical, biophysical and biochemical properties of a cell surface. It is known that most of the cells' surfaces are charged. This charge depends on the biochemical structure of the cell membranes. Therefore, measurement of a cell surface charge is a significant criterion that gives information about the cell surface. Evaluation of the cells zeta-potential is important to understand the interaction mechanisms of various drugs, antibiotics, as well as the interaction of nanoparticles with the cell surface. In this study, we use the dynamic light scattering method to detect the zeta-potential change of Neuro-2a tumor cells. It has been observed that zeta-potential shifted to negative values after exposure to metal oxide nanoparticles and inducing apoptosis.

  18. Regularizations of the Euler product representation for zeta functions and the Birch--Swinnerton-Dyer conjecture

    Fujimoto, Minoru


    We consider a variant expression to regularize the Euler product representation of the zeta functions, where we mainly apply to that of the Riemann zeta function in this paper. The regularization itself is identical to that of the zeta function of the summation expression, but the non-use of the M\\"oebius function enable us to confirm a finite behavior of residual terms which means an absence of zeros except for the critical line. Same technique can be applied to the $L$-function associated to the elliptic curve, and we can deal with the Taylor expansion at the pole in critical strip which is deeply related to the Birch--Swinnerton-Dyer conjecture.

  19. Transgenic overexpression of 14-3-3 zeta protects hippocampus against endoplasmic reticulum stress and status epilepticus in vivo.

    Gary P Brennan

    Full Text Available 14-3-3 proteins are ubiquitous molecular chaperones that are abundantly expressed in the brain where they regulate cell functions including metabolism, the cell cycle and apoptosis. Brain levels of several 14-3-3 isoforms are altered in diseases of the nervous system, including epilepsy. The 14-3-3 zeta (ζ isoform has been linked to endoplasmic reticulum (ER function in neurons, with reduced levels provoking ER stress and increasing vulnerability to excitotoxic injury. Here we report that transgenic overexpression of 14-3-3ζ in mice results in selective changes to the unfolded protein response pathway in the hippocampus, including down-regulation of glucose-regulated proteins 78 and 94, activating transcription factors 4 and 6, and Xbp1 splicing. No differences were found between wild-type mice and transgenic mice for levels of other 14-3-3 isoforms or various other 14-3-3 binding proteins. 14-3-3ζ overexpressing mice were potently protected against cell death caused by intracerebroventricular injection of the ER stressor tunicamycin. 14-3-3ζ overexpressing mice were also potently protected against neuronal death caused by prolonged seizures. These studies demonstrate that increased 14-3-3ζ levels protect against ER stress and seizure-damage despite down-regulation of the unfolded protein response. Delivery of 14-3-3ζ may protect against pathologic changes resulting from prolonged or repeated seizures or where injuries provoke ER stress.

  20. Finding zeros of the Riemann zeta function by periodic driving of cold atoms

    Creffield, C. E.; Sierra, G.


    The Riemann hypothesis, which states that the nontrivial zeros of the Riemann zeta function all lie on a certain line in the complex plane, is one of the most important unresolved problems in mathematics. We propose here an approach to finding a physical system to study the Riemann zeros, which is based on applying a time-periodic driving field. This driving allows us to tune the quasienergies of the system (the analog of the eigenenergies for static systems), so that they are directly governed by the zeta function. We further show by numerical simulations that this allows the Riemann zeros to be measured in currently accessible cold-atom experiments.

  1. Roots of the derivative of the Riemann zeta function and of characteristic polynomials

    Dueñez, Eduardo; Froehlich, Sara; Hughes, Chris; Mezzadri, Francesco; Phan, Toan


    We investigate the horizontal distribution of zeros of the derivative of the Riemann zeta function and compare this to the radial distribution of zeros of the derivative of the characteristic polynomial of a random unitary matrix. Both cases show a surprising bimodal distribution which has yet to be explained. We show by example that the bimodality is a general phenomenon. For the unitary matrix case we prove a conjecture of Mezzadri concerning the leading order behavior, and we show that the same follows from the random matrix conjectures for the zeros of the zeta function.

  2. Analytic renormalization of multiple zeta functions. Geometry and combinatorics of generalized Euler reflection formula for MZV

    Vieru, Andrei


    The renormalization of MZV was until now carried out by algebraic means. In this paper, we show that renormalization in general, and in particular of the multiple zeta functions, is more than just a pure algebraic convention. We give a simple analytic method of computing the regularized values of multiple zeta functions in any dimension for arguments of the form (1,...,1), where the series do not converge. These values happen to be the coefficients of the asymptotic expansion of the inverse G...

  3. Can Time-Dependent Zeta Correlators be Swept under the Rug?

    Boran, Sibel


    We discuss quantum gravitational loop effects to observable quantities such as curvature power spectrum and primordial non-gaussianity of Cosmic Microwave Background (CMB) radiation. We first review the previously shown case where one gets a time dependence for zeta-zeta correlator due to loop corrections. Then we investigate the effect of these loop corrections to primordial non-gaussianity of CMB. We conclude that with a single scalar inflaton one gets a huge value for non-gaussianity which exceeds the observed value by at least 30 orders of magnitude. Finally we discuss the consequences of this result for scalar driven inflationary models.

  4. Zeta Function Regularization in Casimir Effect Calculations and J. S. Dowker's Contribution

    Elizalde, Emilio


    A summary of relevant contributions, ordered in time, to the subject of operator zeta functions and their application to physical issues is provided. The description ends with the seminal contributions of Stephen Hawking and Stuart Dowker and collaborators, considered by many authors as the actual starting point of the introduction of zeta function regularization methods in theoretical physics, in particular, for quantum vacuum fluctuation and Casimir effect calculations. After recalling a number of the strengths of this powerful and elegant method, some of its limitations are discussed. Finally, recent results of the so called operator regularization procedure are presented.

  5. Diacylglycerol kinase theta and zeta isoforms : regulation of activity, protein binding partners and physiological functions

    Los, Alrik Pieter


    Diacylglycerol kinases (DGKs) phosphorylate the second messenger diacylglycerol (DAG) yielding phosphatidic acid (PA). In this thesis, we investigated which structural domains of DGKtheta are required for DGK activity. Furthermore, we showed that DGKzeta binds to and is activated by the Retinoblasto

  6. Diacylglycerol kinase theta and zeta isoforms : regulation of activity, protein binding partners and physiological functions

    Los, Alrik Pieter


    Diacylglycerol kinases (DGKs) phosphorylate the second messenger diacylglycerol (DAG) yielding phosphatidic acid (PA). In this thesis, we investigated which structural domains of DGKtheta are required for DGK activity. Furthermore, we showed that DGKzeta binds to and is activated by the Retinoblasto

  7. Jacob's ladders and the first asymptotic formula for the expression of the sixth order $|\\zeta(1/2+i\\varphi(t)/2)|^4|\\zeta(1/2+it)|^2$

    Moser, Jan


    t is proved in this paper that there is a fine correlation between the values of $|\\zeta(1/2+i\\varphi(t)/2)|^4$ and $|\\zeta(1/2+it)|^2$ which correspond to two segments with gigantic distance each from other. This new asymptotic formula cannot be obtained in known theories of Balasubramanian, Heath-Brown and Ivic.

  8. Identifying prospective antifouling coatings for venturis: Zeta potential measurements of oxides at elevated temperatures

    Jayaweera, P.; Hettiarachchi, S.; Pound, B.G. (SRI International, Menlo Park, CA (United States))


    This report describes the results of research to determine the zeta potential of oxides responsible for fouling of heat transfer, venturi, and other flow measurement device surfaces. Fouling deposits formed on these surfaces reduce mass flow rates of water, causing power reductions of as much as 3 to 4%. There is considerable evidence that deposit formation can be prevented by coating the critical surfaces with a coating material that electrostatically repels the suspended fouling particulates. The zeta potential can be used as a parameter to identify and determine the best coating material to be employed for fouling prevention. The research entailed the development of the equipment necessary for zeta potential measurements, measurement of zeta potentials of oxides and coatings of interest to nuclear power plant applications, and evaluation of candidate coating materials to prevent fouling of surfaces. By this approach, numerous metals and oxides have been identified as potential coating materials. Fouling studies revealed that magnetite does not deposit on tungsten and palladium surfaces, indicating the potential use of these metals for coating venturi surfaces used in nuclear power plants.

  9. Large gaps between consecutive maxima of the Riemann zeta-function on the critical line

    Saker, S H


    In this paper, we derive new lower bounds for the normalized distances between consecutive maxima of the Riemann zeta-function on the critical line subject to the truth of the Riemann hypothesis. The method of our proofs relies on a Sobolev type inequality of one dimension and an Opial type inequality with best possible constants.

  10. A Central Limit Theorem for the Zeroes of the Zeta Function

    Rodgers, Brad


    On the assumption of the Riemann hypothesis, we generalize a central limit theorem of Fujii regarding the number of zeroes of Riemann's Zeta function that lie in a mesoscopic interval. The result mirrors results of Soshnikov and others in random matrix theory.

  11. High-concentration zeta potential measurements using light-scattering techniques.

    Kaszuba, Michael; Corbett, Jason; Watson, Fraser Mcneil; Jones, Andrew


    Zeta potential is the key parameter that controls electrostatic interactions in particle dispersions. Laser Doppler electrophoresis is an accepted method for the measurement of particle electrophoretic mobility and hence zeta potential of dispersions of colloidal size materials. Traditionally, samples measured by this technique have to be optically transparent. Therefore, depending upon the size and optical properties of the particles, many samples will be too concentrated and will require dilution. The ability to measure samples at or close to their neat concentration would be desirable as it would minimize any changes in the zeta potential of the sample owing to dilution. However, the ability to measure turbid samples using light-scattering techniques presents a number of challenges. This paper discusses electrophoretic mobility measurements made on turbid samples at high concentration using a novel cell with reduced path length. Results are presented on two different sample types, titanium dioxide and a polyurethane dispersion, as a function of sample concentration. For both of the sample types studied, the electrophoretic mobility results show a gradual decrease as the sample concentration increases and the possible reasons for these observations are discussed. Further, a comparison of the data against theoretical models is presented and discussed. Conclusions and recommendations are made from the zeta potential values obtained at high concentrations.

  12. Phase diagrams of colloidal spheres with a constant zeta-potential

    Smallenburg, F.|info:eu-repo/dai/nl/313939039; Boon, N.J.H.|info:eu-repo/dai/nl/313960143; Kater, M.; Dijkstra, M.|info:eu-repo/dai/nl/123538807; van Roij, R.H.H.G.|info:eu-repo/dai/nl/152978984


    We study suspensions of colloidal spheres with a constant zeta-potential within Poisson–Boltzmann theory, quantifying the discharging of the spheres with increasing colloid density and decreasing salt concentration. We use the calculated renormalized charge of the colloids to determine their

  13. Zeta potential of Polish copper-bearing shale in the absence and presence of flotation frothers

    Mengsu Peng


    Full Text Available In this paper, zeta potential as a function of pH of copper-bearing shale, which is mined in Poland by KGHM, was investigated. The measurements were conducted in water and aqueous solutions of selected flotation frothers. It was established that for investigated copper-bearing shale, after dispersion in water, the isoelectric point (IEP occurs at pH=3.5. Addition of frothers decreased the IEP on the pH scale and the IEP for butanol was 1.93, for MIBC 2.90 and for eicosaethylene glycol hexadecyl ether (C16E20 2.76. In the case of introducing frothers changed, the zeta potential becomes less negative. An empirical equation, having two adjustable parameters, was used in the paper to approximate the course of the zeta potential-pH curve. The equation showed a very good approximation of the zeta potential of the investigated shale either in water or frother aqueous solutions.

  14. "Armastuse retsepti" tippkokk Zeta Jones ei oska muna keeta / Triin Tael

    Tael, Triin


    Scott Hicksi romantiline komöödiafilm "Armastuse retsept" ("No Reservations"), mille peaosas Walesist pärit näitlejanna Catherine Zeta Jones. Näitlejanna muljeid oma rolliks ettevalmistustest, mille hulka käis ka praktika pärisrestoranis

  15. Preliminary X-ray crystallographic analysis of glutathione transferase zeta 1 (GSTZ1a-1a)

    Boone, Christopher D.; Zhong, Guo; Smeltz, Marci; James, Margaret O., E-mail:; McKenna, Robert, E-mail:


    Crystals of glutathione transferase zeta 1 were grown and shown to diffract X-rays to 3.1 Å resolution. They belonged to space group P1, with unit-cell parameters a = 42.0, b = 49.6, c = 54.6 Å, α = 82.9, β = 69.9, γ = 73.4°.

  16. Molecular characterization of zeta class glutathione S-transferases from Pinus brutia Ten.

    E. Oztetik; F. Kockar; M. Alper; M. Iscan


    Glutathione transferases (GSTs; EC play important roles in stress tolerance and metabolic detoxification in plants. In higher plants, studies on GSTs have focussed largely on agricultural plants. There is restricted information about molecular characterization of GSTs in gymnosperms. To date, only tau class GST enzymes have been characterized from some pinus species. For the first time, the present study reports cloning and molecular characterization of two zeta class GST genes, namely PbGSTZ1 and PbGSTZ2 from Pinus brutia Ten., which is an economically important pine native to the eastern Mediterranean region and have to cope with several environmental stress conditions. The PbGSTZ1 gene was isolated from cDNA, whereas PbGSTZ2 was isolated from genomic DNA. Sequence analysis of PbGSTZ1 and PbGSTZ2 revealed the presence of an open reading frame of 226 amino acids with typical consensus sequences of the zeta class plant GSTs. Protein and secondary structure prediction analysis of two zeta class PbGSTZs have shared common features of other plant zeta class GSTs. Genomic clone, PbGSTZ2 gene, is unexpectedly intronless. Extensive sequence analysis of PbGSTZ2, with cDNA clone, PbGSTZ1, revealed 87% identity at nucleotide and 81% identity at amino acid levels with 41 amino acids differences suggesting that genomic PbGSTZ2 gene might be an allelic or a paralogue version of PbGSTZ1.

  17. Broadway teatrites näeb Denzel Washingtoni ja Catherine Zeta-Jonesi / Andres Laasik

    Laasik, Andres, 1960-2016


    New Yorgis välja antud Tony teatriauhinna pälvisid filminäitlejad Denzel Washington, Catherine Zeta-Jones ja Scarlett Johansson. Parim lavale naasnud näidend - "Piirdeaed", parim uus näidend - draama "Punane", mis räägib läti päritolu maalikunstnikust Mark Rothkost. Parim muusikal - "Memphis"

  18. Directional flow induced by synchronized longitudinal and zeta-potential controlling AC-electrical fields

    Wouden, van der E.J.; Hermes, D.C.; Gardeniers, J.G.E.; Berg, van den A.


    Electroosmotic flow (EOF) in a microchannel can be controlled by electronic control of the surface charge using an electrode embedded in the wall of the channel. By setting a voltage to the electrode, the zeta-potential at the wall can be changed locally. Thus, the electrode acts as a "gate" for liq

  19. The effect of WWTP effluent zeta-potential on direct nanofiltration performance

    Schrader, G.A.; Zwijnenburg, A.; Wessling, Matthias


    Laboratory scale filtration tests utilizing wastewater treatment plants (WWTP) effluent were conducted to investigate fouling and filtration performance of nanofiltration (NF) membranes. The focus of this research is to assess the influence of the zeta-potential of the colloidal fraction in WWTP

  20. Measurable operators and the asymptotics of heat kernels and zeta functions

    Carey, Alan


    In this note we answer some questions inspired by the introduction, by Alain Connes, of the notion of measurable operators using Dixmier traces. These questions concern the relationship of measurability to the asymptotics of $\\zeta-$functions and heat kernels. The answers have remained elusive for some 15 years.

  1. Boundary Conditions for the Maintenance of Memory by PKM[zeta] in Neocortex

    Shema, Reul; Hazvi, Shoshi; Sacktor, Todd C.; Dudai, Yadin


    We report here that ZIP, a selective inhibitor of the atypical protein kinase C isoform PKM[zeta], abolishes very long-term conditioned taste aversion (CTA) associations in the insular cortex of the behaving rat, at least 3 mo after encoding. The effect of ZIP is not replicated by a general serine/threonine protein kinase inhibitor that is…

  2. Broadway teatrites näeb Denzel Washingtoni ja Catherine Zeta-Jonesi / Andres Laasik

    Laasik, Andres, 1960-2016


    New Yorgis välja antud Tony teatriauhinna pälvisid filminäitlejad Denzel Washington, Catherine Zeta-Jones ja Scarlett Johansson. Parim lavale naasnud näidend - "Piirdeaed", parim uus näidend - draama "Punane", mis räägib läti päritolu maalikunstnikust Mark Rothkost. Parim muusikal - "Memphis"

  3. Surface free energy components of glass from ellipsometry and zeta potential measurements

    Chibowski, E.; Hołysz, L.; Kip, Gerhardus A.M.; van Silfhout, Arend; Busscher, H.J.


    Two different experimental approaches based on ellipsometry and zeta potential measurements have been employed to determine the dispersion and polar surface free energy components of glass. From ellipsometry the adsorption isotherms of n-octane and water have been determined, yielding values for the

  4. Certain Subclasses of Analytic and Bi-Univalent Functions Involving Double Zeta Functions

    Saibah Siregar


    Full Text Available In the present paper, we introduce two new subclasses of the functions class Σ of bi-univalent functions involving double zeta functions in the open unit disc U={z:zEC, |z|<1}. The estimates on the coefficients |a2| and |a3| for functions in these new subclasses of the function class Σ are obtained in our investigation.

  5. Fractals of the Julia and Mandelbrot sets of the Riemann $zeta$ Function

    Woon, S C


    Computations of the Julia and Mandelbrot sets of the Riemann zeta function and observations of their properties are made. In the appendix section, a corollary of Voronin's theorem is derived and a scale-invariant equation for the bounds in Goldbach conjecture is conjectured.

  6. "Armastuse retsepti" tippkokk Zeta Jones ei oska muna keeta / Triin Tael

    Tael, Triin


    Scott Hicksi romantiline komöödiafilm "Armastuse retsept" ("No Reservations"), mille peaosas Walesist pärit näitlejanna Catherine Zeta Jones. Näitlejanna muljeid oma rolliks ettevalmistustest, mille hulka käis ka praktika pärisrestoranis

  7. Integrated module inverter using a zeta DC-DC converter with feedforward MPPT (Maximum Power Point Tracking) control; Inversor modulo integrado utilizando um conversor CC-CC zeta com controle MPPT feedforward

    Lopez, Henrique Fioravanti Miguel


    This work presents the study and development of a processing power system that could be used in the connection of renewable energy sources to commercial power grid. The system consists of a ZETA converter associated with a bridge inverter operating at low frequency. The Zeta converter, operating in discontinuous conduction mode (DCM), plays the main role in this arrangement, producing a rectified sinusoidal current waveform synchronized with the electric grid. The function of the full-bridge inverter, connected in cascade with the Zeta converter, is to reverse every 180 deg the current generated by the Zeta converter. Initially it presents the analysis of the Zeta converter operating in DCM, as well as a design criterion. Following by the control strategy and the experimental results for the proposed system are presented and discussed. (author)

  8. Electroviscous effect on fluid drag in a microchannel with large zeta potential.

    Jing, Dalei; Bhushan, Bharat


    The electroviscous effect has been widely studied to investigate the effect of surface charge-induced electric double layers (EDL) on the pressure-driven flow in a micro/nano channel. EDL has been reported to reduce the velocity of fluid flow and increase the fluid drag. Nevertheless, the study on the combined effect of EDL with large zeta potential up to several hundred millivolts and surface charge depenedent-slip on the micro/nano flow is still needed. In this paper, the nonlinear Poisson-Boltzmann equation for electrical potential and ion distribution in non-overlapping EDL is first analytically solved. Then, the modified Navier-Stokes equation for the flow considering the effect of surface charge on the electrical conductivity of the electrolyte and slip length is analytically solved. This analysis is used to study the effect of non-overlapping EDL with large zeta potential on the pressure-driven flow in a microchannel with no-slip and charge-dependent slip conditions. The results show that the EDL leads to an increase in the fluid drag, but that slip can reduce the fluid drag. When the zeta potential is large enough, the electroviscous effect disappears for flow in the microchannel under a no-slip condition. However, the retardation of EDL on the flow and the enhancement of slip on the flow counteract each other under a slip condition. The underlying mechanisms of the effect of EDL with large zeta potential on fluid drag are the high net ionic concentration near the channel wall and the fast decay of electrical potential in the EDL when the zeta potential is large enough.

  9. Random matrices, generalized zeta functions and self-similarity of zero distributions

    Shanker, O [Bitfone Corporation, 32451 Golden Lantern Ste. 301, Laguna Niguel, CA 92677 (United States)


    There is growing evidence for a connection between random matrix theories used in physics and the theory of the Riemann zeta function and L-functions. The theory underlying the location of the zeros of these generalized zeta functions is one of the key unsolved problems. Physicists are interested because of the Hilbert-Polya conjecture, that the non-trivial zeros of the zeta function correspond to the eigenvalues of some positive operator. To complement the continuing theoretical work, it would be useful to study empirically the locations of the zeros by different methods. In this paper we use the rescaled range analysis to study the spacings between successive zeros of these functions. Over large ranges of the zeros the spacings have a Hurst exponent of about 0.095, using sample sizes of 10 000 zeros. This implies that the distribution has a high fractal dimension (1.9), and shows a lot of detailed structure. The distribution is of the anti-persistent fractional Brownian motion type, with a significant degree of anti-persistence. Thus, the high-order zeros of these functions show a remarkable self-similarity in their distribution, over fifteen orders of magnitude for the Riemann zeta function{exclamation_point} We find that the Hurst exponents for the random matrix theories show a different behaviour. A heuristic study of the effect of low-order primes seems to show that this effect is a promising candidate to explain the results that we observe in this study. We study the distribution of zeros for L-functions of conductors 3 and 4, and find that the distribution is similar to that of the Riemann zeta functions.

  10. The 4.36-th moment of the Riemann zeta function

    Radziwill, Maksym


    Conditionally on the Riemann Hypothesis we obtain bounds of the correct order of magnitude for the 2k-th moment of the Riemann zeta function for all positive real k 2; the case of k = 2 corresponds to a classical result of Ingham. We prove our result by establishing a connection between moments with k > 2 and the so-called "twisted fourth moment". This allows us to appeal to a recent result of Hughes and Young. Furthermore we obtain a point-wise bound for |zeta(1/2 + it)|^{2r} (with 0 < r < 1) that can be regarded as a multiplicative analogue of Selberg's bound for S(T). We also establish asymptotic formulae for moments (k < 2.181) slightly off the half-line.

  11. Non-Commutative Integration, Zeta Functions and the Haar State for SU{sub q}(2)

    Matassa, Marco, E-mail: [SISSA (Italy)


    We study a notion of non-commutative integration, in the spirit of modular spectral triples, for the quantum group SU{sub q}(2). In particular we define the non-commutative integral as the residue at the spectral dimension of a zeta function, which is constructed using a Dirac operator and a weight. We consider the Dirac operator introduced by Kaad and Senior and a family of weights depending on two parameters, which are related to the diagonal automorphisms of SU{sub q}(2). We show that, after fixing one of the parameters, the non-commutative integral coincides with the Haar state of SU{sub q}(2). Moreover we can impose an additional condition on the zeta function, which also fixes the second parameter. For this unique choice the spectral dimension coincides with the classical dimension.

  12. Non-Commutative Integration, Zeta Functions and the Haar State for SU q (2)

    Matassa, Marco


    We study a notion of non-commutative integration, in the spirit of modular spectral triples, for the quantum group SU q (2). In particular we define the non-commutative integral as the residue at the spectral dimension of a zeta function, which is constructed using a Dirac operator and a weight. We consider the Dirac operator introduced by Kaad and Senior and a family of weights depending on two parameters, which are related to the diagonal automorphisms of SU q (2). We show that, after fixing one of the parameters, the non-commutative integral coincides with the Haar state of SU q (2). Moreover we can impose an additional condition on the zeta function, which also fixes the second parameter. For this unique choice the spectral dimension coincides with the classical dimension.

  13. Phase diagrams of colloidal spheres with a constant zeta-potential.

    Smallenburg, Frank; Boon, Niels; Kater, Maarten; Dijkstra, Marjolein; van Roij, René


    We study suspensions of colloidal spheres with a constant zeta-potential within Poisson-Boltzmann theory, quantifying the discharging of the spheres with increasing colloid density and decreasing salt concentration. We use the calculated renormalized charge of the colloids to determine their pairwise effective screened-Coulomb repulsions. Bulk phase diagrams in the colloid concentration-salt concentration representation follow, for various zeta-potentials, by a mapping onto published fits of phase boundaries of point-Yukawa systems. Although the resulting phase diagrams do feature face-centered cubic and body-centered cubic phases, they are dominated by the (re-entrant) fluid phase due to the colloidal discharging with increasing colloid concentration and decreasing salt concentration.

  14. On the higher derivatives of Z(t) associated with the Riemann Zeta-Function

    Matsuoka, Kaneaki


    Let $Z(t)$ be the classical Hardy function in the theory of the Riemann zeta-function. The main result in this paper is that if the Riemann hypothesis is true then for any positive integer $n$ there exists a $t_{n}>0$ such that for $t>t_{n}$ the function $Z^{(n+1)}(t)$ has exactly one zero between consecutive zeros of $Z^{(n)}(t)$.

  15. Trace of heat kernel,spectral zeta function and isospectral problem for sub-laplacians

    CHANG; Der-Chen; YEUNG; Sai-Kee


    In this article,we first study the trace for the heat kernel for the sub-Laplacian operator on the unit sphere in C n+1.Then we survey some results on the spectral zeta function which is induced by the trace of the heat kernel.In the second part of the paper,we discuss an isospectral problem in the CR setting.

  16. The Stability of Electron Orbital Shells based on a Model of the Riemann-Zeta Function

    Harney M.


    Full Text Available It is shown that the atomic number Z is prime at the beginning of the each s1, p1, d1, and f1 energy levels of electrons, with some fluctuation in the actinide and lanthanide series. The periodic prime number boundary of s1, p1, d1, and f1 is postulated to occur because of stability of Schrodinger’s wave equation due to a fundamental relationship with the Riemann-Zeta function.

  17. New Relations Involving an Extended Multiparameter Hurwitz-Lerch Zeta Function with Applications

    H. M. Srivastava


    Full Text Available We derive several new expansion formulas involving an extended multiparameter Hurwitz-Lerch zeta function introduced and studied recently by Srivastava et al. (2011. These expansions are obtained by using some fractional calculus methods such as the generalized Leibniz rules, the Taylor-like expansions in terms of different functions, and the generalized chain rule. Several (known or new special cases are also given.

  18. Variations of the Ramanujan polynomials and remarks on $\\zeta(2j+1)/\\pi^{2j+1}$

    Lalin, Matilde


    We observe that five polynomial families have all of their zeros on the unit circle. We prove the statements explicitly for four of the polynomial families. The polynomials have coefficients which involve Bernoulli numbers, Euler numbers, and the odd values of the Riemann zeta function. These polynomials are closely related to the Ramanujan polynomials, which were recently introduced by Murty, Smyth and Wang. Our proofs rely upon theorems of Schinzel, and Lakatos and Losonczi and some generalizations.

  19. Computational estimation of the constant beta (1) characterizing the order of zeta (1+it)

    Kotnik, Tadej


    The paper describes a computational estimation of the constant beta (1) characterizing the bounds of left\\vert zeta (1+it)right\\vert . It is known that as trightarrow infty frac{zeta (2)}{2beta (1)e^{gamma }left[ 1+o(1)right] log \\... ... (1+it)right\\vert leq 2beta (1)e^{gamma }left[ 1+o(1) right] log log t with beta (1)geq frac{1}{2} , while the truth of the Riemann hypothesis would also imply that beta (1)leq 1 . In the range 1zeta (1+it)right\\vert . As t increases, the estimates in the first set rapidly fall below 1 and gradually reach values slightly below 0.70 , while the estimates in the second set rapidly exceed frac{1}{2} and gradually reach values slightly above 0.64 . The obtained numerical results are discussed and compared to the implications of recent theoretical work of Granville and Soundararajan.

  20. A perturbative approach to the spectral zeta functions of strings, drums, and quantum billiards

    Amore, Paolo [Facultad de Ciencias, CUICBAS, Universidad de Colima, Bernal Diaz del Castillo 340, Colima, Colima (Mexico)


    We show that the spectral zeta functions of inhomogeneous strings and drums can be calculated using Rayleigh-Schroedinger perturbation theory. The inhomogeneities that can be treated with this method are small but otherwise arbitrary and include the previously studied case of a piecewise constant density. In two dimensions the method can be used to derive the spectral zeta function of a domain obtained from the small deformation of a square. We also obtain exact sum rules that are valid for arbitrary densities and that correspond to the values taken by the spectral zeta function at integer positive values; we have tested numerically these sum rules in specific examples. We show that the Dirichlet or Neumann Casimir energies of an inhomogeneous string, evaluated to first order in perturbation theory, contain in some cases an irremovable divergence, but that the combination of the two is always free of divergences. Finally, our calculation of the Casimir energies of a string with piecewise constant density and of two perfectly conducting concentric cylinders, of similar radius, reproduce the results previously published.

  1. Electrokinetic mixing at high zeta potentials: ionic size effects on cross stream diffusion.

    Ahmadian Yazdi, Alireza; Sadeghi, Arman; Saidi, Mohammad Hassan


    The electrokinetic phenomena at high zeta potentials may show several unique features which are not normally observed. One of these features is the ionic size (steric) effect associated with the solutions of high ionic concentration. In the present work, attention is given to the influences of finite ionic size on the cross stream diffusion process in an electrokinetically actuated Y-shaped micromixer. The method consists of a finite difference based numerical approach for non-uniform grid which is applied to the dimensionless form of the governing equations, including the modified Poisson-Boltzmann equation. The results reveal that, neglecting the ionic size at high zeta potentials gives rise to the overestimation of the mixing length, because the steric effects retard liquid flow, thereby enhancing the mixing efficiency. The importance of steric effects is found to be more intense for channels of smaller width to height ratio. It is also observed that, in sharp contrast to the conditions that the ions are treated as point charges, increasing the zeta potential improves the cross stream diffusion when incorporating the ionic size. Moreover, increasing the EDL thickness decreases the mixing length, whereas the opposite is true for the channel aspect ratio.

  2. Tetracycline adsorption on chitosan: a mechanistic description based on mass uptake and zeta potential measurements.

    Caroni, A L P F; de Lima, C R M; Pereira, M R; Fonseca, J L C


    Adsorption phenomena occurring at the solid/liquid interface of chitosan particles are of extreme importance in the kinetics of drug release/upload as well as in effluent treatment by adsorption. In this work, equilibrium and kinetic aspects of protonated tetracycline adsorption on chitosan are explored using classic solution depletion method and zeta potential measurements. Equilibrium experiments showed that for solutions with tetracycline initial concentration of ca. 1.2 g L(-1), corresponding to a pH around 3, chitosan structure disrupted, as indicated by an increase in magnitude of tetracycline sorption. Adsorption and zeta potential isotherms before disruption suggested that the process of adsorption had a Langmuir character up to a point at which subsurface was exposed to adsorption; at this point, a second mode of sorption began: zeta potential tended to an equilibrium value, following Sips isotherm and tetracycline sorption had a linear dependence on its continuous phase concentration. The kinetics of tetracycline sorption suggested that sorption of tetracycline was divided between the sorption of protonated and non-protonated tetracycline; tetracycline in its non-protonated form seemed to rule the sorption of tetracycline.

  3. Fractal zeta functions and fractal drums higher-dimensional theory of complex dimensions

    Lapidus, Michel L; Žubrinić, Darko


    This monograph gives a state-of-the-art and accessible treatment of a new general higher-dimensional theory of complex dimensions, valid for arbitrary bounded subsets of Euclidean spaces, as well as for their natural generalization, relative fractal drums. It provides a significant extension of the existing theory of zeta functions for fractal strings to fractal sets and arbitrary bounded sets in Euclidean spaces of any dimension. Two new classes of fractal zeta functions are introduced, namely, the distance and tube zeta functions of bounded sets, and their key properties are investigated. The theory is developed step-by-step at a slow pace, and every step is well motivated by numerous examples, historical remarks and comments, relating the objects under investigation to other concepts. Special emphasis is placed on the study of complex dimensions of bounded sets and their connections with the notions of Minkowski content and Minkowski measurability, as well as on fractal tube formulas. It is shown for the f...

  4. Spectral zeta function and non-perturbative effects in ABJM Fermi-gas

    Hatsuda, Yasuyuki


    The exact partition function in ABJM theory on three-sphere can be regarded as a canonical partition function of a non-interacting Fermi-gas with an unconventional Hamiltonian. All the information on the partition function is encoded in the discrete spectrum of this Hamiltonian. We explain how (quantum mechanical) non-perturbative corrections in the Fermi-gas system appear from a spectral consideration. Basic tools in our analysis are a Mellin-Barnes type integral representation and a spectral zeta function. From a consistency with known results, we conjecture that the spectral zeta function in the ABJM Fermi-gas has an infinite number of "non-perturbative" poles, which are invisible in the semi-classical expansion of the Planck constant. We observe that these poles indeed appear after summing up perturbative corrections. As a consequence, the perturbative resummation of the spectral zeta function causes non-perturbative corrections to the grand canonical partition function. We also present another example associated with a spectral problem in topological string theory. A conjectured non-perturbative free energy on the resolved conifold is successfully reproduced in this framework.

  5. Spectral zeta function and non-perturbative effects in ABJM Fermi-gas

    Hatsuda, Yasuyuki


    The exact partition function in ABJM theory on three-sphere can be regarded as a canonical partition function of a non-interacting Fermi-gas with an unconventional Hamiltonian. All the information on the partition function is encoded in the discrete spectrum of this Hamiltonian. We explain how (quantum mechanical) non-perturbative corrections in the Fermi-gas system appear from a spectral consideration. Basic tools in our analysis are a Mellin-Barnes type integral representation and a spectral zeta function. From a consistency with known results, we conjecture that the spectral zeta function in the ABJM Fermi-gas has an infinite number of "non-perturbative" poles, which are invisible in the semi-classical expansion of the Planck constant. We observe that these poles indeed appear after summing up perturbative corrections. As a consequence, the perturbative resummation of the spectral zeta function causes non-perturbative corrections to the grand canonical partition function. We also present another example as...

  6. Hydrophobicity, surface tension, and zeta potential measurements of glass-reinforced hydroxyapatite composites.

    Lopes, M A; Monteiro, F J; Santos, J D; Serro, A P; Saramago, B


    Wettability and zeta potential studies were performed to characterize the hydrophobicity, surface tension, and surface charge of P2O5-glass-reinforced hydroxyapatite composites. Quantitative phase analysis was performed by the Rietveld method using GSAS software applied to X-ray diffractograms. Surface charge was assessed by zeta potential measurements. Protein adsorption studies were performed using vitronectin. Contact angles and surface tensions variation with time were determined by the sessile and pendent drop techniques, respectively, using ADSA-P software. The highest (-18.1 mV) and lowest (-28.7 mV) values of zeta potential were found for hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP), respectively, with composite materials presenting values in between. All studied bioceramic materials showed similar solid surface tension. For HA and beta-TCP, solid surface tensions of 46.7 and 45.3 mJ/m2, respectively, were obtained, while composites presented intermediate surface tension values. The dispersive component of surface tension was the predominant one for all materials studied. Adhesion work values between the vitronectin solution and HA and beta-TCP were found to be 79.8 and 88.0 mJ/m2, respectively, while the 4.0 wt % glass composites showed slightly lower values than the 2.5 wt % ones. The presence of beta-TCP influenced surface charge, hydrophobicity, and protein adsorption of the glass-reinforced HA composites, and therefore indirectly affected cell-biomaterial interactions.

  7. Relation between primes and nontrivial zeros in the Riemann hypothesis; Legendre polynomials, modified zeta function and Schrödinger equation

    Choi, Seongsoo; Chung, J. W.; Kim, Kwang S.


    We study the dependence between prime numbers and the real and imaginary parts of the nontrivial zeros of the Riemann zeta function. The Legendre polynomials and the partial derivatives of the Riemann zeta function are used to investigate the above dependence along with the Riemann hypothesis with physical interpretations. A modified zeta function with finite terms is defined as a new implement for the study of the zeta function and its zeros.

  8. Integral and Series Representations of Riemann's Zeta Function and Dirichlet's Eta Function and a Medley of Related Results

    Michael S. Milgram


    Full Text Available Contour integral representations of Riemann's Zeta function and Dirichlet's Eta (alternating Zeta function are presented and investigated. These representations flow naturally from methods developed in the 1800s, but somehow they do not appear in the standard reference summaries, textbooks, or literature. Using these representations as a basis, alternate derivations of known series and integral representations for the Zeta and Eta function are obtained on a unified basis that differs from the textbook approach, and results are developed that appear to be new.

  9. Quantum corrections to finite-gap solutions for Yang-Mills-Nahm equations via zeta-function technique

    Leble, Sergey


    One-dimensional Yang-Mills-Nahm models are considered from algebrogeometric points of view. A quasiclassical quantization of the models based on path integral and its zeta function representation in terms of a Green function diagonal for a heat equation with an elliptic potential is considered. The Green function diagonal and, hence, zeta function and its derivative are expressed via solutions of Hermit equation and, alternatively, by means of Its-Matveev formalism in terms of Riemann teta-functions. For the Nahm model, which field is represented via elliptic (lemniscate) integral by construction, one-loop quantum corrections to action are evaluated as the zeta function derivative in zero point in terms of a hyperelliptic integral. The alternative expression should help to link the representations and continue investigation of the Yang-Mills-Nahm models. Keywords: Nahm model, one-loop quantum corrections, zeta function, elliptic potential, hyperelliptic integral, Its-Matveev formula. MSC numbers: 81Q30, 35J10...

  10. CD3zeta down-modulation may explain Vgamma9Vdelta2 T lymphocyte anergy in HIV-infected patients.

    Sacchi, Alessandra; Tempestilli, Massimo; Turchi, Federica; Agrati, Chiara; Casetti, Rita; Cimini, Eleonora; Gioia, Cristiana; Martini, Federico


    The aim of the present study was to explain the observed anergy of Vgamma9Vdelta2 T cells from human immunodeficiency virus (HIV)-positive patients. CD3zeta expression and interferon (IFN)-gamma production by Vgamma9Vdelta2 T cells from HIV-positive and HIV-negative subjects were analyzed. We demonstrated that Vgamma9Vdelta2 T cells from HIV-infected patients expressed a lower level of CD3zeta than did Vgamma9Vdelta2 T cells from healthy donors. A direct correlation was found between CD3zeta expression and IFN-gamma production capability by Vgamma9Vdelta2 T cells. However, activation of protein kinase C by phorbol myristate acetate is able to restore CD3zeta expression and IFN-gamma production. Our findings may contribute to clarification of the molecular mechanisms of Vgamma9Vdelta2 T cell anergy found in HIV-positive patients.

  11. On the distribution of extreme values of zeta and $L$-functions in the strip $1/2<\\sigma<1$

    Lamzouri, Youness


    We study the distribution of large (and small) values of several families of $L$-functions on a line $\\text{Re(s)}=\\sigma$ where $1/2<\\sigma<1$. We consider the Riemann zeta function $\\zeta(s)$ in the $t$-aspect, Dirichlet $L$-functions in the $q$-aspect, and $L$-functions attached to primitive holomorphic cusp forms of weight $2$ in the level aspect. For each family we show that the $L$-values can be very well modeled by an adequate random Euler product, uniformly in a wide range. We also prove new $\\Omega$-results for quadratic Dirichlet $L$-functions (predicted to be best possible by the probabilistic model) conditionally on GRH, and other results related to large moments of $\\zeta(\\sigma+it)$.

  12. On an analytic estimate in the theory of the Riemann zeta function and a theorem of Báez-Duarte.

    Burnol, Jean-François


    On the Riemann hypothesis we establish a uniform upper estimate for zeta(s)/zeta (s + A), 0 Riemann Hypothesis. We investigate function-theoretically some of the functions defined by Báez-Duarte in his study and we show that their square-integrability is, in itself, an equivalent formulation of the Riemann Hypothesis. We conclude with a third equivalent formulation which resembles a "causality" statement.

  13. A Derivation of $pi(n$ Based on a Stability Analysis of the Riemann-Zeta Function

    Harney M.


    Full Text Available The prime-number counting function $pi(n$, which is significant in the prime number theorem, is derived by analyzing the region of convergence of the real-part of the Riemann-Zeta function using the unilateral $z$-transform. In order to satisfy the stability criteria of the $z$-transform, it is found that the real part of the Riemann-Zeta function must converge to the prime-counting function.

  14. A Derivation of π(n Based on a Stability Analysis of the Riemann-Zeta Function

    Harney M.


    Full Text Available The prime-number counting function ( n , which is significant in the prime number the- orem, is derived by analyzing the region of convergence of the real-part of the Riemann- Zeta function using the unilateral z -transform. In order to satisfy the stability criteria of the z -transform, it is found that the real part of the Riemann-Zeta function must con- verge to the prime-counting function.

  15. The ClpXP protease is responsible for the degradation of the Epsilon antidote to the Zeta toxin of the streptococcal pSM19035 plasmid.

    Brzozowska, Iwona; Zielenkiewicz, Urszula


    Most bacterial genomes contain different types of toxin-antitoxin (TA) systems. The ω-ε-ζ proteinaceous type II TA cassette from the streptococcal pSM19035 plasmid is a member of the ε/ζ family, which is commonly found in multiresistance plasmids and chromosomes of various human pathogens. Regulation of type II TA systems relies on the proteolysis of antitoxin proteins. Under normal conditions, the Epsilon antidote neutralizes the Zeta toxin through the formation of a tight complex. In this study, we show, using both in vivo and in vitro analyses, that the ClpXP protease is responsible for Epsilon antitoxin degradation. Using in vivo studies, we examined the stability of the plasmids with active or inactive ω-ε-ζ TA cassettes in B. subtilis mutants that were defective for different proteases. Using in vitro assays, the degradation of purified His6-Epsilon by the His6-LonBs, ClpPBs, and ClpXBs proteases from B. subtilis was analyzed. Additionally, we showed that purified Zeta toxin protects the Epsilon protein from rapid ClpXP-catalyzed degradation.

  16. Silver ion impregnated composite biomaterial optimally prepared using zeta potential measurements.

    Sakthivel, N; Socrates, R; Shanthini, G M; Rajaram, A; Kalkura, S Narayana


    Biodegradable, antimicrobial composite of various silver ion concentrations was synthesized using zeta potential and isoelectric point measurements, for a controlled release of silver ions, and in addition to assess the effect of protein adsorption with the increase of the silver ion concentration. The interaction between hydroxyapatite (HAp) and silver incorporated hydroxyapatite (AgHAp) with gelatin was increased by optimally adjusting the zeta potential and isoelectric point of the ceramic (HAp and AgHAp), and bio-polymer individually. The electrostatic interactions between the ceramic and biopolymer were confirmed, through shifts in N-H stretching, decrease in the swelling ratio, and increase in the degradation temperature observed by the derivative thermo-gravimetric analysis (DTG). These results substantiate that, the zeta potential is a novel tool to increase the ceramic-biopolymer interaction. Increasing electrostatic interaction between the biopolymer and ceramic, decreases the release of silver ions in the simulated body fluid, due to the controlled degradation of the biopolymer. The isoelectric point decreases with the increase of the silver ion concentration, which evidenced the change in the net surface charge. With the increase of the silver ion concentration, the protein adsorption decreases due to an increase in hydrophilic character of the composite. This study examines the minimum concentration of silver ion essential for maximum protein adsorption, antimicrobial and hemocompatibility. This study provides a novel route to control the release of silver ions by enhancing the ceramic-polymer interaction and estimate the silver ion concentration suitable for protein adsorption. The prepared composite is nontoxic, degradable, and antimicrobial, with the controlled release of silver ions in the simulated body fluid.

  17. Quantum Graphs Whose Spectra Mimic the Zeros of the Riemann Zeta Function

    Kuipers, Jack; Hummel, Quirin; Richter, Klaus


    One of the most famous problems in mathematics is the Riemann hypothesis: that the nontrivial zeros of the Riemann zeta function lie on a line in the complex plane. One way to prove the hypothesis would be to identify the zeros as eigenvalues of a Hermitian operator, many of whose properties can be derived through the analogy to quantum chaos. Using this, we construct a set of quantum graphs that have the same oscillating part of the density of states as the Riemann zeros, offering an explanation of the overall minus sign. The smooth part is completely different, and hence also the spectrum, but the graphs pick out the low-lying zeros.

  18. Horizontal Monotonicity of the Modulus of the Riemann Zeta Function and Related Functions

    Matiyasevich, Yuri; Zvengrowski, Peter


    It is shown that the absolute values of Riemann's zeta function and two related functions strictly decrease when the imaginary part of the argument is fixed to any number with absolute value at least 8 and the real part of the argument is negative and increases up to 0; extending this monotonicity to the increase of the real part up to 1/2 is shown to be equivalent to the Riemann Hypothesis. Another result is a double inequality relating the real parts of the logarithmic derivatives of the three functions under consideration.

  19. Relations between elliptic multiple zeta values and a special derivation algebra

    Broedel, Johannes; Matthes, Nils; Schlotterer, Oliver


    We investigate relations between elliptic multiple zeta values (eMZVs) and describe a method to derive the number of indecomposable elements of given weight and length. Our method is based on representing eMZVs as iterated integrals over Eisenstein series and exploiting the connection with a special derivation algebra. Its commutator relations give rise to constraints on the iterated integrals over Eisenstein series relevant for eMZVs and thereby allow to count the indecomposable representatives. Conversely, the above connection suggests apparently new relations in the derivation algebra. Under we provide relations for eMZVs over a wide range of weights and lengths.

  20. Time-series photometry of the O4 I(n)fp star zeta Puppis

    Howarth, Ian D.; Stevens, Ian R.


    We report a time-series analysis of the O4 I(n)fp star zeta Pup, based on optical photometry obtained with the SMEI instrument on the Coriolis satellite, 2003--2006. A single astrophysical signal is found, with P = (1.780938 \\pm 0.000093) d and a mean semi-amplitude of (6.9 \\pm 0.3) mmag. There is no evidence for persistent coherent signals with semi-amplitudes in excess of ca. 2~mmag on any of the timescales previously reported in the literature. In particular, there is no evidence for a sig...

  1. Quantum scalar fields in the half-line. A heat kernel/zeta function approach

    Mateos Guilarte, Juan; Muñoz-Castañeda, Jose María; Senosiaín Aramendía, María Jesús


    [EN]In this paper we shall study vacuum fluctuations of a single scalar field with Dirichlet boundary conditions in a finite but very long line. The spectral heat kernel, the heat partition function and the spectral zeta function are calculated in terms of Riemann Theta functions, the error function, and hypergeometric PFQ functions. [ES]En este artículo vamos a estudiar las fluctuaciones en el vacío de un campo escalar con las condiciones de contorno de Dirichlet en una línea finita pero muy...

  2. Applications of Wirtinger Inequalities on the Distribution of Zeros of the Riemann Zeta-Function

    Saker SamirH


    Full Text Available On the hypothesis that the th moments of the Hardy -function are correctly predicted by random matrix theory and the moments of the derivative of are correctly predicted by the derivative of the characteristic polynomials of unitary matrices, we establish new large spaces between the zeros of the Riemann zeta-function by employing some Wirtinger-type inequalities. In particular, it is obtained that which means that consecutive nontrivial zeros often differ by at least 6.1392 times the average spacing.

  3. Quantum graphs whose spectra mimic the zeros of the Riemann zeta function.

    Kuipers, Jack; Hummel, Quirin; Richter, Klaus


    One of the most famous problems in mathematics is the Riemann hypothesis: that the nontrivial zeros of the Riemann zeta function lie on a line in the complex plane. One way to prove the hypothesis would be to identify the zeros as eigenvalues of a Hermitian operator, many of whose properties can be derived through the analogy to quantum chaos. Using this, we construct a set of quantum graphs that have the same oscillating part of the density of states as the Riemann zeros, offering an explanation of the overall minus sign. The smooth part is completely different, and hence also the spectrum, but the graphs pick out the low-lying zeros.

  4. Graph Zeta Function in the Bethe Free Energy and Loopy Belief Propagation

    Watanabe, Yusuke


    We propose a new approach to the analysis of Loopy Belief Propagation (LBP) by establishing a formula that connects the Hessian of the Bethe free energy with the edge zeta function. The formula has a number of theoretical implications on LBP. It is applied to give a sufficient condition that the Hessian of the Bethe free energy is positive definite, which shows non-convexity for graphs with multiple cycles. The formula clarifies the relation between the local stability of a fixed point of LBP and local minima of the Bethe free energy. We also propose a new approach to the uniqueness of LBP fixed point, and show various conditions of uniqueness.

  5. Fractional parts and their relations to the values of the Riemann zeta function

    Alabdulmohsin, Ibrahim


    A well-known result, due to Dirichlet and later generalized by de la Vallée–Poussin, expresses a relationship between the sum of fractional parts and the Euler–Mascheroni constant. In this paper, we prove an asymptotic relationship between the summation of the products of fractional parts with powers of integers on the one hand, and the values of the Riemann zeta function, on the other hand. Dirichlet’s classical result falls as a particular case of this more general theorem.

  6. The Structural Evolution of Milky-Way-Like Star-Forming Galaxies zeta is approximately 1.3

    Patel, Shannon G.; Fumagalli, Mattia; Franx, Marun; VanDokkum, Pieter G.; VanDerWel, Arjen; Leja, Joel; Labbe, Ivo; Brammr, Gabriel; Whitaker, Katherine E.; Skelton, Rosalind E.; Momcheva, Ivelina; Lundgren, Britt; Muzzin, Adam; Quadri, Ryan F.; Nelson, Erica June; Wake, David A.; Rix, Hans-Walter


    We follow the structural evolution of star-forming galaxies (SFGs) like the Milky Way by selecting progenitors to zeta is approx. 1.3 based on the stellar mass growth inferred from the evolution of the star-forming sequence. We select our sample from the 3D-HT survey, which utilizes spectroscopy from the HST-WFC3 G141 near-IR grism and enables precise redshift measurements for our sample of SFGs. Structural properties are obtained from Sersic profile fits to CANDELS WFC3 imaging. The progenitors of zeta = 0 SFGs with stellar mass M = 10(exp 10.5) solar mass are typically half as massive at zeta is approx. 1. This late-time stellar mass grow is consistent with recent studies that employ abundance matching techniques. The descendant SFGs at zeta is approx. 0 have grown in half-light radius by a factor of approx. 1.4 zeta is approx. 1. The half-light radius grows with stellar mass as r(sub e) alpha stellar mass(exp 0.29). While most of the stellar mass is clearly assembling at large radii, the mass surface density profiles reveal ongoing mass growth also in the central regions where bulges and pseudobulges are common features in present day late-type galaxies. Some portion of this growth in the central regions is due to star formation as recent observations of H(a) maps for SFGs at zeta approx. are found to be extended but centrally peaked. Connecting our lookback study with galactic archeology, we find the stellar mass surface density at R - 8 kkpc to have increased by a factor of approx. 2 since zeta is approx. 1, in good agreement with measurements derived for the solar neighborhood of the Milky Way.

  7. Stability of fenbendazole suspensions for veterinary use. Correlation between zeta potential and sedimentation.

    Arias, José L; López-Viota, Margarita; Clares, Beatriz; Ruiz, Ma Adolfina


    In this paper we have carried out a detailed investigation of the stability and redispersibility characteristics of fenbendazole aqueous suspensions, through a thermodynamic and electrokinetic characterization, considering the effect of both pH and ionic strength. The hydrophobic character of the drug, and the surface charge and electrical double-layer thickness play an essential role in the stability of the system, hence the need for a full characterization of fenbendazole. It was found that the drug suspensions displays "delayed" or "hindered" sedimentation, determined by their hydrophobic character and their low zeta potential (indicating a small electrokinetic charge on the particles). The electrostatic repulsion between the particles is responsible for the low sedimentation volume and poor redispersibility of the drug. However, only low concentrations of AlCl(3) induced a significant effect on both the zeta potential and stability of the drug, leading to a "free-layered" sedimentation and a very easy redispersion which could be of great interest in the design of an oral pharmaceutical dosage form for veterinary.

  8. Hilbert-P\\'olya Conjecture, Zeta-Functions and Bosonic Quantum Field Theories

    Andrade, Julio


    The original Hilbert and P\\'olya conjecture is the assertion that the non-trivial zeros of the Riemann zeta function can be the spectrum of a self-adjoint operator. So far no such operator was found. However the suggestion of Hilbert and P\\'olya, in the context of spectral theory, can be extended to approach other problems and so it is natural to ask if there is a quantum mechanical system related to other sequences of numbers which are originated and motivated by Number Theory. In this paper we show that the functional integrals associated with a hypothetical class of physical systems described by self-adjoint operators associated with bosonic fields whose spectra is given by three different sequence of numbers cannot be constructed. The common feature of the sequence of numbers considered here, which causes the impossibility of zeta regularization, is that the various Dirichlet series attached to such sequences - such as those which are sums over "primes" of $(\\mathrm{norm} \\ P)^{-s}$ have a natural boundar...

  9. Electroosmotic fluid motion and late-time solute transport at non-negligible zeta potentials

    S. K. Griffiths; R. H. Nilson


    Analytical and numerical methods are employed to determine the electric potential, fluid velocity and late-time solute distribution for electroosmotic flow in a tube and channel when the zeta potential is not small. The electric potential and fluid velocity are in general obtained by numerical means. In addition, new analytical solutions are presented for the velocity in a tube and channel in the extremes of large and small Debye layer thickness. The electroosmotic fluid velocity is used to analyze late-time transport of a neutral non-reacting solute. Zeroth and first-order solutions describing axial variation of the solute concentration are determined analytically. The resulting expressions contain eigenvalues representing the dispersion and skewness of the axial concentration profiles. These eigenvalues and the functions describing transverse variation of the concentration field are determined numerically using a shooting technique. Results are presented for both tube and channel geometries over a wide range of the normalized Debye layer thickness and zeta potential. Simple analytical approximations to the eigenvalues are also provided for the limiting cases of large and small values of the Debye layer thickness. The methodology developed here for electroosmotic flow is also applied to the Taylor problem of late-time transport and dispersion in pressure-driven flows.

  10. The Zeta Potential of Surface-Functionalized Metallic Nanorod Particles in Aqueous Solution

    Dougherty, G M; Rose, K A; Tok, J B; Pannu, S S; Chuang, F S; Sha, M Y; Chakarova, G; Penn, S G


    Metallic nanoparticles suspended in aqueous solutions, and functionalized with chemical and biological surface coatings, are important elements in basic and applied nanoscience research. Many applications require an understanding of the electrokinetic or colloidal properties of such particles. In this paper we describe the results of experiments to measure the zeta potential of metallic nanorod particles in aqueous saline solutions, including the effects of pH, ionic strength, metallic composition, and surface functionalization state. Particle substrates tested include gold, silver, and palladium monometallic particles as well as gold/silver bimetallic particles. Surface functionalization conditions included 11-mercaptoundecanoic acid (MUA), mercaptoethanol (ME), and mercaptoethanesulfonic acid (MESA) self-assembled monolayers (SAMs), as well as MUA layers subsequently derivatized with proteins. Zeta potential data for typical charge-stabilized polystyrene particles are also presented for comparison. Experimental data are compared with theory. The results of these studies are useful in predicting and controlling the aggregation, adhesion, and transport of functionalized metallic nanoparticles within microfluidic devices and other systems.

  11. The Zeta-Function of a p-Adic Manifold, Dwork Theory for Physicists

    Candelas, Philip


    In this article we review the observation, due originally to Dwork, that the zeta-function of an arithmetic variety, defined originally over the field with p elements, is a superdeterminant. We review this observation in the context of a one parameter family of quintic threefolds, and study the zeta-function as a function of the parameter \\phi. Owing to cancellations, the superdeterminant of an infinite matrix reduces to the (ordinary) determinant of a finite matrix, U(\\phi), corresponding to the action of the Frobenius map on certain cohomology groups. The parameter-dependence of U(\\phi) is given by a relation U(\\phi)=E^{-1}(\\phi^p)U(0)E(\\phi) with E(\\phi) a Wronskian matrix formed from the periods of the manifold. The periods are defined by series that converge for $|\\phi|_p < 1$. The values of \\phi that are of interest are those for which \\phi^p = \\phi so, for nonzero \\phi, we have |\\vph|_p=1. We explain how the process of p-adic analytic continuation applies to this case. The matrix U(\\phi) breaks up i...

  12. Multiple zeta functions and double wrapping in planar N=4 SYM

    Leurent, Sébastien


    Using the FiNLIE solution of the AdS/CFT Y-system, we compute the anomalous dimension of the Konishi operator in planar N=4 SYM up to eight loops, i.e. up to the leading double wrapping order. At this order a non reducible Euler-Zagier sum, zeta(1,2,8), appears for the first time. We find that at all orders in perturbation, every spectral-dependent quantity of the Y-system is expressed through multiple Hurwitz zeta functions, hence we provide a Mathematica package to manipulate these functions, including the particular case of Euler-Zagier sums. Furthermore, we conjecture that only Euler-Zagier sums can appear in the answer for the anomalous dimension at any order in perturbation theory. We also resum the leading transcendentality terms of the anomalous dimension at all orders, obtaining a simple result in terms of Bessel functions. Finally, we demonstrate that exact Bethe equations should be related to an absence of poles condition that becomes especially nontrivial at double wrapping.

  13. Effective flocculation of Chlorella vulgaris using chitosan with zeta potential measurement

    Low, Y. J.; Lau, S. W.


    Microalgae are considered as one promising source of third-generation biofuels due to their fast growth rates, potentially higher yield rates and wide ranges of growth conditions. However, the extremely low biomass concentration in microalgae cultures presents a great challenge to the harvesting of microalgae because a large volume of water needs to be removed to obtain dry microalgal cells for the subsequent oil extraction process. In this study, the fresh water microalgae Chlorella vulgaris (C. vulgaris) was effectively harvested using both low molecular weight (MW) and high MW chitosan flocculants. The flocculation efficiency was evaluated by physical appearance, supernatant absorbance, zeta potential and solids content after centrifugal dewatering. High flocculation efficiency of 98.0-99.0% was achieved at the optimal dosage of 30-40 mg/g with formation of large microalgae flocs. This study suggests that the polymer bridging mechanism was governing the flocculation behaviour of C. vulgaris using high MW chitosan. Besides, charge patch neutralisation mechanism prevailed at low MW chitosan where lower dosage was sufficient to reach near-zero zeta potential compared with the high MW chitosan. The amount of chitosan polymer present in the culture may also affect the mechanism of flocculation.

  14. Surface electrochemical properties of red mud (bauxite residue): zeta potential and surface charge density.

    Liu, Yanju; Naidu, Ravendra; Ming, Hui


    The surface electrochemical properties of red mud (bauxite residue) from different alumina refineries in Australia and China were studied by electrophoresis and measuring surface charge density obtained from acid/base potentiometric titrations. The electrophoretic properties were measured from zeta potentials obtained in the presence of 0.01 and 0.001 M KNO(3) over a wide pH range (3.5-10) by titration. The isoelectric point (IEP) values were found to vary from 6.35 to 8.70 for the red mud samples. Further investigation into the surface charge density of one sample (RRM) by acid/base potentiometric titration showed similar results for pH(PZC) with pH(IEP) obtained from electrokinetic measurements. The pH(IEP) determined from zeta potential measurements can be used as a characteristic property of red mud. The minerals contained in red mud contributed to the different values of pH(IEP) of samples obtained from different refineries. Different relationships of pH(IEP) with Al/Fe and Al/Si ratios (molar basis) were also found for different red mud samples.

  15. Fundamental stellar parameters of $\\zeta$ Pup and $\\gamma^2$ Vel from HIPPARCOS data

    Schärer, D; Grenon, Michel; Schaerer, Daniel; Schmutz, Werner; Grenon, Michel


    We report parallax measurements by the HIPPARCOS satellite of zeta Puppis and gamma^2 Velorum. The distance of zeta Pup is d=429 (+120/ -77) pc, in agreement with the commonly adopted value to Vela OB2. However, a significantly smaller distance is found for the gamma^2 Vel system: d=258 (+41/-31) pc. The total mass of gamma^2 Vel derived from its parallax, the angular size of the semi-major axis as measured with intensity interferometry, and the period is M(WR+O)=29.5 (+/-15.9) Msun. This result favors the orbital solution of Pike et al. (1983) over that of Moffat et al. (1986). The stellar parameters for the O star companion derived from line blanketed non-LTE atmosphere models are: Teff=34000 (+/-1500) K, log L/Lsun=5.3 (+/-0.15) from which an evolutionary mass of M=29 (+/-4) Msun and an age of 4.0 (+0.8/-0.5) Myr is obtained from single star evolutionary models. With non-LTE model calculations including He and C we derive a luminosity log L/Lsun~4.7 (+/-0.2) for the WR star. The mass-luminosity relation of...

  16. Nanoparticle Tracking Analysis for Determination of Hydrodynamic Diameter, Concentration, and Zeta-Potential of Polyplex Nanoparticles.

    Wilson, David R; Green, Jordan J


    Nanoparticle tracking analysis (NTA) is a recently developed nanoparticle characterization technique that offers certain advantages over dynamic light scattering for characterizing polyplex nanoparticles in particular. Dynamic light scattering results in intensity-weighted average measurements of nanoparticle characteristics. In contrast, NTA directly tracks individual particles, enabling concentration measurements as well as the direct determination of number-weighted particle size and zeta-potential. A direct number-weighted assessment of nanoparticle characteristics is particularly useful for polydisperse samples of particles, including many varieties of gene delivery particles that can be prone to aggregation. Here, we describe the synthesis of poly(beta-amino ester)/deoxyribonucleic acid (PBAE/DNA) polyplex nanoparticles and their characterization using NTA to determine hydrodynamic diameter, zeta-potential, and concentration. Additionally, we detail methods of labeling nucleic acids with fluorophores to assess only those polyplex nanoparticles containing plasmids via NTA. Polymeric gene delivery of exogenous plasmid DNA has great potential for treating a wide variety of diseases by inducing cells to express a gene of interest.

  17. Newton flow of the Riemann zeta function: separatrices control the appearance of zeros

    Neuberger, J. W.; Feiler, C.; Maier, H.; Schleich, W. P.


    A great many phenomena in physics can be traced back to the zeros of a function or a functional. Eigenvalue or variational problems prevalent in classical as well as quantum mechanics are examples illustrating this statement. Continuous descent methods taken with respect to the proper metric are efficient ways to attack such problems. In particular, the continuous Newton method brings out the lines of constant phase of a complex-valued function. Although the patterns created by the Newton flow are reminiscent of the field lines of electrostatics and magnetostatics they cannot be realized in this way since in general they are not curl-free. We apply the continuous Newton method to the Riemann zeta function and discuss the emerging patterns emphasizing especially the structuring of the non-trivial zeros by the separatrices. This approach might open a new road toward the Riemann hypothesis.

  18. Explicit Formulae for Values of Dedekind Zeta Functions of Two Kinds of Cyclotomic Fields

    马连荣; 张贤科


    @@ Let K = Q(ζm) denote the m-th cyclotomic field, and K+ its maximal real subfield, where ζm =exp( ) is an m-th primary root of unity. Let ζK(s) denote the Dedekind zeta function of K. For prime integers m = p, Fumio Hazama recently in [1] obtained formulae for calculating special values of ζK(s) and ζK+(s), i.e., calculating formulae of ζK+(1 -- n) and ζK(1-n) for ζK+ (1-n)positive integers n, which are the newest results of a series of his work in many years (see [1-3]).

  19. Combinatorics of lower order terms in the moment conjectures for the Riemann zeta function

    Dehaye, Paul-Olivier


    Conrey, Farmer, Keating, Rubinstein and Snaith have given a recipe that conjecturally produces, among others, the full moment polynomial for the Riemann zeta function. The leading term of this polynomial is given as a product of a factor explained by arithmetic and a factor explained by combinatorics (or, alternatively, random matrices). We explain how the lower order terms arise, and clarify the dependency of each factor on the exponent $k$ that is considered. We use extensively the theory of symmetric functions and representations of symmetric groups, ideas of Lascoux on manipulations of alphabets, and a key lemma, due in a basic version to Bump and Gamburd. Our main result ends up involving dimensions of partitions, as studied by Olshanski, Regev, Vershik, Ivanov and others.

  20. Influence of zeta potential on the flocculation of cyanobacteria cells using chitosan modified soil.

    Li, Liang; Zhang, Honggang; Pan, Gang


    Using chitosan modified soil to flocculate and sediment algal cells has been considered as a promising strategy to combat cyanobacteria blooms in natural waters. However, the flocculation efficiency often varies with algal cells with different zeta potential (ZP) attributed to different growth phases or water conditions. This article investigated the relationship between ZP of Microcystis aeruginosa and its influence to the flocculation efficiency using chitosan modified soil. Results suggested that the optimal removal efficiency was obtained when the ZP was between -20.7 and -6.7 mV with a removal efficiency of more than 80% in 30 min and large floc size of >350 μm. When the algal cells were more negatively charged than -20.7 mV, the effect of chitosan modified soil was depressed (flocculation efficiency of chitosan modified soil and an important guidance for practical engineering of cyanobacteria bloom control.

  1. Simultaneous visible and ultraviolet spectroscopy of stellar wind variability in Zeta Puppis

    Wegner, G. A.; Snow, T. P., Jr.


    Spectra of He II 4686 A and H-alpha in Zeta Pup were obtained simultaneously with Copernicus ultraviolet scans of several P Cygni profiles in this O4 If supergiant with strong mass loss. The visible-wavelength data show significant variations in the profiles of both lines, consisting of doubling of the emission over times of less than a day. Recent theoretical calculations show that the observed profile variations in 4686 A can be produced by significant fluctuations in the wind density. The Copernicus data show less variability, although in one scan of the 1400-A Si IV doublet there appears to be a significant enhancement of the emission which may be correlated with one of the doublings observed in 4686 A and H-alpha, as though a density enhancement formed at low levels and then moved outward in the wind.

  2. Measuring microchannel electroosmotic mobility and zeta potential by the current monitoring method.

    Shao, Chenren; Devoe, Don L


    Electroosmotic flow (EOF) is an electrokinetic flow control technique widely used in microfluidic systems for applications including direct electrokinetic pumping, hydrodynamic pressure generation, and counterflow for microfluidic separations. During EOF, an electric field is applied along the length of a microchannel containing an electrolyte, with mobile ions near the charged microchannel walls experiencing a Coulomb force due to electrostatic interactions with the applied electric field that leads to bulk solution movement. The goal of this laboratory is to experimentally determine the fixed channel surface charge (zeta potential) and electroosmotic mobility associated with a given microchannel substrate material and buffer solution, using a simple current monitoring method to measure the average flow velocity within the microchannel. It is a straightforward experiment designed to help students understand EOF physics while gaining hands-on experience with basic world-to-chip interfacing. It is well suited to a 90-min laboratory session for up to 12 students with minimal infrastructure requirements.

  3. Measuring differential rotation of the K-giant $\\zeta$\\,And

    K\\Hovári, Zs; Kriskovics, L; Vida, K; Donati, J -F; Coroller, H Le; Pedretti, J D Monnier E; Petit, P


    We investigate the temporal spot evolution of the K-giant component in the RS CVn-type binary system $\\zeta$\\,Andromedae to establish its surface differential rotation. Doppler imaging is used to study three slightly overlapping spectroscopic datasets, obtained independently at three different observing sites. Each dataset covers one full stellar rotation with good phase coverage, and in total, results in a continuous coverage of almost three stellar rotations ($P_{\\rm rot}=$17.8\\,d). Therefore, these data are well suited for reconstructing surface temperature maps and studying temporal evolution in spot configurations. Surface differential rotation is measured by the means of cross-correlation of all the possible image pairs. The individual Doppler reconstructions well agree in the revealed spot pattern, recovering numerous low latitude spots with temperature contrasts of up to $\\approx$1000\\,K with respect to the unspotted photosphere, and also an asymmetric polar cap which is diminishing with time. Our det...




    Full Text Available In the past 10 years, the need for biodegradable lubricants has been more and more emphasized. The use of vegetable oils as lubricants offers several advantages. The vegetable oils are biodegradable; thus, the environmental pollution is minimal either during or after their use. The aim of this paper is to presents a preliminary study concerning the influence of some preparation conditions on the stability of vegetable oil-in-water (O/W emulsions as eco-friendly lubricants stabilized by nonionic surfactant. In this context, vegetable oil-in-water emulsions characteristics where assessed using microscopically observation and zeta potential. In addition, the color of these emulsions can be evaluated. It can be observed that the emulsions tend to stabilize in time.

  5. Surfactants, not size or zeta-potential influence blood-brain barrier passage of polymeric nanoparticles.

    Voigt, Nadine; Henrich-Noack, Petra; Kockentiedt, Sarah; Hintz, Werner; Tomas, Jürgen; Sabel, Bernhard A


    Nanoparticles (NP) can deliver drugs across the blood-brain barrier (BBB), but little is known which of the factors surfactant, size and zeta-potential are essential for allowing BBB passage. To this end we designed purpose-built fluorescent polybutylcyanoacrylate (PBCA) NP and imaged the NP's passage over the blood-retina barrier - which is a model of the BBB - in live animals. Rats received intravenous injections of fluorescent PBCA-NP fabricated by mini-emulsion polymerisation to obtain various NP's compositions that varied in surfactants (non-ionic, anionic, cationic), size (67-464nm) and zeta-potential. Real-time imaging of retinal blood vessels and retinal tissue was carried out with in vivo confocal neuroimaging (ICON) before, during and after NP's injection. Successful BBB passage with subsequent cellular labelling was achieved if NP were fabricated with non-ionic surfactants or cationic stabilizers but not when anionic compounds were added. NP's size and charge had no influence on BBB passage and cell labelling. This transport was not caused by an unspecific opening of the BBB because control experiments with injections of unlabelled NP and fluorescent dye (to test a "door-opener" effect) did not lead to parenchymal labelling. Thus, neither NP's size nor chemo-electric charge, but particle surface is the key factor determining BBB passage. This result has important implications for NP engineering in medicine: depending on the surfactant, NP can serve one of two opposite functions: while non-ionic tensides enhance brain up-take, addition of anionic tensides prevents it. NP can now be designed to specifically enhance drug delivery to the brain or, alternatively, to prevent brain penetration so to reduce unwanted psychoactive effects of drugs or prevent environmental nanoparticles from entering tissue of the central nervous system.

  6. Mechanisms of fibrinogen adsorption on latex particles determined by zeta potential and AFM measurements.

    Adamczyk, Zbigniew; Bratek-Skicki, Anna; Dąbrowska, Paulina; Nattich-Rak, Małgorzata


    The adsorption of fibrinogen on polystyrene latex particles was studied using the concentration depletion method combined with the AFM detection of residual protein after adsorption. Measurements were carried out for a pH range of 3.5-11 and an ionic strength range of 10(-3)-0.15 M NaCl. First, the bulk physicochemical properties of fibrinogen and the latex particle suspension were characterized for this range of pH and ionic strength. The zeta potential and the number of uncompensated (electrokinetic) charges on the protein were determined from microelectrophoretic measurements. It was revealed that fibrinogen molecules exhibited amphoteric characteristics, being on average positively charged for pH adsorption of fibrinogen on latex for pH below 11. It was also proven that fibrinogen adsorption was irreversible, with the maximum surface concentration varying between 2.5 and 5 × 10(3) μm(-2) (weight concentration of a bare molecule was 1.4 to 2.8 mg m(-2)). These measurements revealed two main adsorption mechanisms of fibrinogen: (i) the unoriented (random) mechanism prevailing for lower ionic strength, where adsorbing molecules significantly penetrate the fuzzy polymeric layer on the latex core and (ii) the side-on adsorption mechanism prevailing for pH > 5.8 and a higher ionic strength of 0.15 M. It was also shown that in the latter case, variations in the zeta potential with the protein coverage could be adequately described in terms of the electrokinetic model, previously formulated for planar substrate adsorption. On the basis of these experimental data, an efficient procedure of preparing fibrinogen-covered latex particles of controlled monolayer structure and coverage was envisaged.

  7. Calculations of Isoelectronic Series of He Using Noninteger n-Slater Type Orbitals in Single and Double Zeta Approximations

    GUSEINOV,Israfil; ERT(U)RK,Murat; SAHIN,Ercan; AKSU,Hüseyin


    Using integer and noninteger n-Slater type orbitals in single- and double-zeta approximations, the Hartree-Fock-Roothaan calculations were performed for the ground states of first ten cationic members of the isoelectronic series of He atom. All the noninteger parameters and orbital exponents were fully optimized. In the case of noninteger n-Slater type orbitals in double zeta basis sets, the results of calculations obtained are more close to the numerical Hatree-Fock values and the average deviations of our ground state energies do not exceed 2×10-6 hartrees of their numerical results.

  8. Uncoupling of T Cell Receptor Zeta Chain Function during the Induction of Anergy by the Superantigen, Staphylococcal Enterotoxin A

    William D. Cornwell


    Full Text Available Staphylococcus aureus enterotoxins have immunomodulatory properties. In this study, we show that Staphylococcal enterotoxin A (SEA induces a strong proliferative response in a murine T cell clone independent of MHC class II bearing cells. SEA stimulation also induces a state of hypo-responsiveness (anergy. We characterized the components of the T cell receptor (TCR during induction of anergy by SEA. Most interestingly, TCR zeta chain phosphorylation was absent under SEA anergizing conditions, which suggests an uncoupling of zeta chain function. We characterize here a model system for studying anergy in the absence of confounding costimulatory signals.

  9. Development of a novel in silico model of zeta potential for metal oxide nanoparticles: a nano-QSPR approach

    Wyrzykowska, Ewelina; Mikolajczyk, Alicja; Sikorska, Celina; Puzyn, Tomasz


    Once released into the aquatic environment, nanoparticles (NPs) are expected to interact (e.g. dissolve, agglomerate/aggregate, settle), with important consequences for NP fate and toxicity. A clear understanding of how internal and environmental factors influence the NP toxicity and fate in the environment is still in its infancy. In this study, a quantitative structure-property relationship (QSPR) approach was employed to systematically explore factors that affect surface charge (zeta potential) under environmentally realistic conditions. The nano-QSPR model developed with multiple linear regression (MLR) was characterized by high robustness ({{{Q}}{{2}}}{{CV}}=0.90) and external predictivity ({{{Q}}{{2}}}{{EXT}}=0.93). The results clearly showed that zeta potential values varied markedly as functions of the ionic radius of the metal atom in the metal oxides, confirming that agglomeration and the extent of release of free MexOy largely depend on their intrinsic properties. A developed nano-QSPR model was successfully applied to predict zeta potential in an ionized solution of NPs for which experimentally determined values of response have been unavailable. Hence, the application of our model is possible when the values of zeta potential in the ionized solution for metal oxide nanoparticles are undetermined, without the necessity of performing more time consuming and expensive experiments. We believe that our studies will be helpful in predicting the conditions under which MexOy is likely to become problematic for the environment and human health.

  10. Effect of isotonic solutions and peptide adsorption on zeta potential of porous silicon nanoparticle drug delivery formulations.

    Kaasalainen, Martti; Mäkilä, Ermei; Riikonen, Joakim; Kovalainen, Miia; Järvinen, Kristiina; Herzig, Karl-Heinz; Lehto, Vesa-Pekka; Salonen, Jarno


    Recently, highly promising results considering the use of porous silicon (PSi) nanoparticles as a controlled and targeted drug delivery system have been published. Drugs are typically loaded into PSi nanoparticles by electrostatic interactions, and the drug-loaded nanoparticles are then administered parenterally in isotonic solutions. Zeta potential has an important role in drug adsorption and overall physical stability of nanosuspensions. In the present study, we used zeta potential measurements to study the impact of the formulation components to the nanosuspension stability. The impact of medium was studied by measuring isoelectric points (IEP) and zeta potentials in isotonic media. The role of drug adsorption was demonstrated with gastrointestinal peptides GLP-1(7-37) and PYY (3-36) and the selection of isotonic additive was demonstrated with peptide-loaded PSi nanoparticles. The results show the notable effect of isotonic solutions and peptide adsorption on zeta potential of PSi nanosuspensions. As a rule of thumb, the sugars (sucrose, dextrose and mannitol) seem to be good media for negatively charged peptide-loaded particles and weak acids (citric- and lactic acid) for positively charged particles. Nevertheless, perhaps the most important rule can be given for isotonic salt solutions which all are very poor media when the stability of nanosuspension is considered.

  11. ζ-potential determination using a ZetaMeter-Dynamic Speckle assembly

    González-Peña, Rolando J.; Sánchez-Muñoz, Orlando L.; Martínez-Celorio, René A.; Cibrián, Rosa M.; Salvador-Palmer, Rosario; Salgado, Jesús


    Electrophoretic mobility and ζ-potential are important physical parameters for the characterization of micro- and nanosystems. In this communication we describe a new method for determining the ζ-potential through the assembly of two well known techniques: free electrophoresis and Dynamic Speckle. When coherent light passes through a fluid having scattering centres, the far field interference originates a speckled image. If the scattering centres are contained within the cylindrical electrophoresis cell of a ZetaMeter and are forced to move in an orderly way under the action of an external electric field, the time variation of the light intensity in the far field speckle images follows a temporal autocorrelation function g(τ). The corresponding correlation time can then be obtained and related with the velocity, from which the electrophoretic mobility and the ζ-potential of the scattering centres can be determined. We have applied this method to microparticles, like natural air-floated silica and two classes of bioceramics, hydroxyapatite and biphasic calcium phosphate. For comparison, we analysed the same samples in parallel using a commercial Zetasizer Nano from Malvern Instruments. The values of ζ-potential determined using the two techniques were the same within ~3% error. These results validate our new method as a useful and efficient alternative for ζ-potential determination of particles, at least within the micrometer scale.

  12. Giants of eclipse the ζ [Zeta] Aurigae stars and other binary systems

    Griffin, Elizabeth


    The zeta Aurigae stars are the rare but illustrious sub-group of binary stars that undergo the dramatic phenomenon of "chromospheric eclipse". This book provides detailed descriptions of the ten known systems, illustrates them richly with examples of new spectra, and places them in the context of stellar structure and evolution. Comprised of a large cool giant plus a small hot dwarf, these key eclipsing binaries reveal fascinating changes in their spectra very close to total eclipse, when the hot star shines through differing heights of the "chromosphere", or outer atmosphere, of the giant star. The phenomenon provides astrophysics with the means of analyzing the outer atmosphere of a giant star and how that material is shed into space. The physics of these critical events can be explained qualitatively, but it is more challenging to extract hard facts from the observations, and tough to model the chromosphere in any detail. The book offers current thinking on mechanisms for heating a star's chromosphere an...

  13. Reduced fertilization after ICSI and abnormal phospholipase C zeta presence in spermatozoa from the wobbler mouse.

    Heytens, Elke; Schmitt-John, Thomas; Moser, Jakob M; Jensen, Nanna Mandøe; Soleimani, Reza; Young, Claire; Coward, Kevin; Parrington, John; De Sutter, Petra


    Failed fertilization after intracytoplasmic sperm injection (ICSI) can be due to a reduced oocyte-activation capacity caused by reduced concentrations and abnormal localization of the oocyte-activation factor phospholipase C (PLC) zeta. Patients with this condition can be helped to conceive by artificial activation of oocytes after ICSI with calcium ionophore (assisted oocyte activation; AOA). However some concern still exists about this approach. Mouse models could help to identify potential oocyte-activation strategies and evaluate their safety. In this study, the fertilizing capacity of wobbler sperm cells was tested and the efficiency of AOA with two exposures to ionomycin to restore fertilization and embryo development was studied. The quality of the obtained blastocysts was assessed and embryo transfer was performed to evaluate post-implantation development. The presence of PLCzeta in the spermatozoa and testis of the wobbler mouse was evaluated by PLCzeta immunostaining and quantitative reverse-transcription polymerase chain reaction. Sperm cells from wobbler mice had reduced fertilizing capacity and abnormalities in PLCzeta localization, but not in its expression. Artificially activating the oocytes restored fertilization and embryo development. Therefore, the wobbler mouse can be a model for failed fertilization after ICSI to study PLCzeta dynamics and aid in optimization of the AOA method.

  14. TC Trends And Terrestrial Planet Formation: The Case of Zeta Reticuli

    Vardan, Adibekyan; Delgado-Mena, Elisa; Figueira, Pedro; Sousa, Sergio; Santos, Nuno; Faria, Joao; González Hernández, Jonay; Israelian, Garik; Harutyunyan, Gohar; Suárez-Andrés, Lucia; Hakobyan, Arthur


    During the last decade astronomers have been trying to search for chemical signatures of terrestrial planet formation in the atmospheres of the hosting stars. Several studies suggested that the chemical abundance trend with the condensation temperature, Tc, is a signature of rocky planet formation. In particular, it was suggested that the Sun shows 'peculiar' chemical abundances due to the presence of the terrestrial planets in our solar-system. However, the rocky material accretion or the trap of rocky materials in terrestrial planets is not the only explanation for the chemical 'peculiarity' of the Sun, or other Sun-like stars with planets. In this talk I madea very brief review of this topic, and presented our last results for the particular case of Zeta Reticuli binary system: A very interesting and well-known system (known in science fiction and ufology as the world of Grey Aliens, or Reticulans) where one of the components hosts an exo-Kuiper belt, and the other component is a 'single', 'lonely' star.

  15. Hippocampal Infusion of Zeta Inhibitory Peptide Impairs Recent, but Not Remote, Recognition Memory in Rats

    Jena B. Hales


    Full Text Available Spatial memory in rodents can be erased following the infusion of zeta inhibitory peptide (ZIP into the dorsal hippocampus via indwelling guide cannulas. It is believed that ZIP impairs spatial memory by reversing established late-phase long-term potentiation (LTP. However, it is unclear whether other forms of hippocampus-dependent memory, such as recognition memory, are also supported by hippocampal LTP. In the current study, we tested recognition memory in rats following hippocampal ZIP infusion. In order to combat the limited targeting of infusions via cannula, we implemented a stereotaxic approach for infusing ZIP throughout the dorsal, intermediate, and ventral hippocampus. Rats infused with ZIP 3–7 days after training on the novel object recognition task exhibited impaired object recognition memory compared to control rats (those infused with aCSF. In contrast, rats infused with ZIP 1 month after training performed similar to control rats. The ability to form new memories after ZIP infusions remained intact. We suggest that enhanced recognition memory for recent events is supported by hippocampal LTP, which can be reversed by hippocampal ZIP infusion.

  16. Zeta potential, contact angles, and AFM imaging of protein conformation adsorbed on hybrid nanocomposite surfaces.

    Pinho, Ana C; Piedade, Ana P


    The sputtering deposition of gold (Au) and poly(tetrafluoroethylene) (PTFE) was used to prepare a nanocomposite hybrid thin film suitable for protein adsorption while maintaining the native conformation of the biological material. The monolithic PTFE and the nanocomposite PTFE/Au thin films, with Au content up to 1 at %, were co-deposited by r.f. magnetron sputtering using argon as a discharge gas and deposited onto 316L stainless steel substrates, the most commonly used steel in biomaterials. The deposited thin films, before and after bovine serum albumin (BSA) adsorption, were thoroughly characterized with special emphasis on the surface properties/characteristics by atomic force microscopy (AFM), zeta potential, and static and dynamic contact angle measurements, in order to assess the relationship between structure and conformational changes. The influence of a pre-adsorbed peptide (RGD) was also evaluated. The nanotopographic and chemical changes induced by the presence of gold in the nanocomposite thin films enable RGD bonding, which is critical for the maintenance of the BSA native conformation after adsorption.

  17. Perancangan Zeta Converter yang dilengkapi Power Factor Correction pada Aplikasi Pengaturan Kecepatan Motor Brushless DC

    Adhika Prajna Nandiwardhana


    Full Text Available Penggunaan motor brushless DC telah banyak digunakan dalam berbagai bidang seperti peralatan rumah tangga maupun industri dikarenakan motor ini memiliki struktur yang sederhana, efisiensi dan torsi yang tinggi, serta menggunakan konsep komutasi elektris yang berbeda dari motor DC lainnya. Namun pengoperasian pada umumnya yang menggunakan sumber AC, penyearah serta inverter membuat tingginya nilai harmonisa arus (THD sebesar 73,33% dan power factor sebesar 0,803 dimana nilai ini kurang baik dalam pengaplikasiannya. Pada penelitian ini akan dikaji mengenai proses power factor correction yang mereduksi harmonisa arus (THD sumber AC dengan menggunakan zeta converter dalam pengaplikasian motor brushless DC, serta pengoperasian motor dengan mengamati respon motor terhadap kecepatan referensi yang berubah-ubah dan mengamati kestabilan motor terhadap pembebanan yang bervariasi. Dalam menerapkan metode yang dilakukan pada penelitian ini, pengoperasian motor brushless DC yang telah dirancang dapat bekerja dengan baik meliputi respon motor yang dapat mengikuti kecepatan referensi yang berubah-ubah, serta kestabilan motor dalam mempertahankan kecepatannya pada pembebanan yang bervariasi. Proses power factor correction dapat meningkatkan kualitas daya pada berbagai kecepatan dan mode penerapan yang berbeda-beda, dimana peningkatan tersebut membuktikan kinerja yang baik dalam sistem ini dan memiliki nilai kualitas daya yang baik.

  18. Interrelationship between the zeta potential and viscoelastic properties in coacervates complexes.

    Espinosa-Andrews, Hugo; Enríquez-Ramírez, Karina Esmeralda; García-Márquez, Eristeo; Ramírez-Santiago, Cesar; Lobato-Calleros, Consuelo; Vernon-Carter, Jaime


    The formation of the complex coacervate (CC) phases between gum Arabic (GA) and low molecular weight chitosan (Ch) and the interrelationship between the zeta-potential and viscoelastic properties of the coacervate phase were investigated. The maximum charge difference of biopolymers stock dispersion was displayed in a range of pH between 4.0 and 5.5. Titration experiment between the oppositely charged biopolymers showed that the isoelectric point was found at a biopolymers mass ratio (R[GA:Ch]) of R[5.5:1]. Turbidity, size and ζ-potential of the soluble complexes (SC) showed an interrelation with the complex coacervate yield (CCY). Higher CCY values (82.2-88.1%) were obtained in the range from R[3:1] to R[5.5:1]. Change the R[GA:Ch] in dispersion, make possible to produce CC's phases exhibiting cationic (R[1:1] and R[3:1]), neutral (R[5.5:1]) or anionic (R[9:1] and R[7:1]) charged. All CC's exhibited liquid-viscoelastic behavior at lower frequencies and a crossover between G″ and G' at higher frequencies.

  19. Universal Dielectric Enhancement from Externally Induced Double Layer Without $\\zeta$-Potential

    Qian, Jiang


    Motivated by recent experiments showing over $10^4$-fold increase in induced polarization from electrochemically inert, conducting materials in dilute saline solutions, we theoretically demonstrate a new mechanism for dielectric enhancement, in the absence of $\\zeta-$potentials at interfaces between non-insulating particles and an electrolyte solution. We further show that the magnitude of such enhancement obeys universal scaling laws, independent of the particle's electrical properties and valid across particle shapes: for a dilute suspension of identical, but arbitrarily shaped particles of a linear dimension $a$ and volume fraction $f$, as $\\omega\\to0$ the effective real dielectric constant of the mixture is enhanced from that of water by a factor $1+f~(P_r+(a/\\lambda)P_i)$, and the frequency-dependent phase shift of its impedance has a scale-invariant maximum $f\\,\\mathsf{\\Theta}$ if particles are much more conductive than the solution. Here $\\lambda$ is the solution's Debye length and $P_r$, $P_i$, $\\math...

  20. Dynamical mass of the O-type supergiant in Zeta Orionis A

    Hummel, C A; Nieva, M -F; Stahl, O; van Belle, G; Zavala, R T


    A close companion of Zeta Orionis A was found in 2000 with the Navy Precision Optical Interferometer (NPOI), and shown to be a physical companion. Because the primary is a supergiant of type O, for which dynamical mass measurements are very rare, the companion was observed with NPOI over the full 7-year orbit. Our aim was to determine the dynamical mass of a supergiant that, due to the physical separation of more than 10 AU between the components, cannot have undergone mass exchange with the companion. The interferometric observations allow measuring the relative positions of the binary components and their relative brightness. The data collected over the full orbital period allows all seven orbital elements to be determined. In addition to the interferometric observations, we analyzed archival spectra obtained at the Calar Alto, Haute Provence, Cerro Armazones, and La Silla observatories, as well as new spectra obtained at the VLT on Cerro Paranal. In the high-resolution spectra we identified a few lines tha...

  1. Local zeta regularization and the scalar Casimir effect I. A general approach based on integral kernels

    Fermi, Davide


    This is the first one of a series of papers about zeta regularization of the divergences appearing in the vacuum expectation value (VEV) of several local and global observables in quantum field theory. More precisely we consider a quantized, neutral scalar field on a domain in any spatial dimension, with arbitrary boundary conditions and, possibly, in presence of an external classical potential. We analyze, in particular, the VEV of the stress-energy tensor, the corresponding boundary forces and the total energy, thus taking into account both local and global aspects of the Casimir effect. In comparison with the wide existing literature on these subjects, we try to develop a more systematic approach, allowing to treat specific configurations by mere application of a general machinery. The present Part I is mainly devoted to setting up this general framework; at the end of the paper, this is exemplified in a very simple case. In Parts II, III and IV we will consider more engaging applications, indicated in the...

  2. Beyond the triangle and uniqueness relations non-zeta counterterms at large N from positive knots

    Broadhurst, D J; Kreimer, D


    Counterterms that are not reducible to $\\zeta_{n}$ are generated by ${}_3F_2$ hypergeometric series arising from diagrams for which triangle and uniqueness relations furnish insufficient data. Irreducible double sums, corresponding to the torus knots $(4,3)=8_{19}$ and $(5,3)=10_{124}$, are found in anomalous dimensions at ${\\rm O}(1/N^3)$ in the large-$N$ limit, which we compute analytically up to terms of level 11, corresponding to 11 loops for 4-dimensional field theories and 12 loops for 2-dimensional theories. High-precision numerical results are obtained up to 24 loops and used in Padé resummations of $\\varepsilon$-expansions, which are compared with analytical results in 3 dimensions. The ${\\rm O}(1/N^3)$ results entail knots generated by three dressed propagators in the master two-loop two-point diagram. At higher orders in $1/N$ one encounters the uniquely positive hyperbolic 11-crossing knot, associated with an irreducible triple sum. At 12 crossings, a pair of 3-braid knots is generated, correspon...

  3. Characterization of PF4-Heparin Complexes by Photon Correlation Spectroscopy and Zeta Potential.

    Bertini, Sabrina; Fareed, Jawed; Madaschi, Laura; Risi, Giulia; Torri, Giangiacomo; Naggi, Annamaria


    Heparin-induced thrombocytopenia (HIT) is associated with antibodies to complexes between heparin and platelet factor 4 (PF4), a basic protein usually found in platelet alpha granules. Heparin-induced thrombocytopenia antibodies preferentially recognize macromolecular complexes formed between positively charged PF4 and polyanionic heparins over a narrow range of molar ratios. The aim of this work was to study the complexes that human PF4 forms with heparins from various species, such as porcine, bovine, and ovine; heparins from various organs, such as mucosa and lung; and different low-molecular-weight heparins (LMWHs) at several stoichiometric ratios to evaluate their sizes and charges by photo correlation spectroscopy and zeta potential measurements. The resulting data of the PF4 complexes with unfractionated heparins (UFHs), LMWHs and their fractions, and oligosaccharide components suggest that the size of aggregates is not only a simple function of average molecular weight but also of the molecular weight distribution of the sample. Moreover, it was found that lower concentrations of the tested ovine-derived mucosal heparin are required to form the large PF4/heparin complexes as compared to mucosal porcine and bovine heparin.

  4. Hippocampal Infusion of Zeta Inhibitory Peptide Impairs Recent, but Not Remote, Recognition Memory in Rats

    Hales, Jena B.; Ocampo, Amber C.; Broadbent, Nicola J.; Clark, Robert E.


    Spatial memory in rodents can be erased following the infusion of zeta inhibitory peptide (ZIP) into the dorsal hippocampus via indwelling guide cannulas. It is believed that ZIP impairs spatial memory by reversing established late-phase long-term potentiation (LTP). However, it is unclear whether other forms of hippocampus-dependent memory, such as recognition memory, are also supported by hippocampal LTP. In the current study, we tested recognition memory in rats following hippocampal ZIP infusion. In order to combat the limited targeting of infusions via cannula, we implemented a stereotaxic approach for infusing ZIP throughout the dorsal, intermediate, and ventral hippocampus. Rats infused with ZIP 3–7 days after training on the novel object recognition task exhibited impaired object recognition memory compared to control rats (those infused with aCSF). In contrast, rats infused with ZIP 1 month after training performed similar to control rats. The ability to form new memories after ZIP infusions remained intact. We suggest that enhanced recognition memory for recent events is supported by hippocampal LTP, which can be reversed by hippocampal ZIP infusion. PMID:26380123

  5. Moments of zeta functions associated to hyperelliptic curves over finite fields.

    Rubinstein, Michael O; Wu, Kaiyu


    Let q be an odd prime power, and Hq,d denote the set of square-free monic polynomials D(x)∈Fq[x] of degree d. Katz and Sarnak showed that the moments, over Hq,d, of the zeta functions associated to the curves y(2)=D(x), evaluated at the central point, tend, as q→∞, to the moments of characteristic polynomials, evaluated at the central point, of matrices in USp(2⌊(d-1)/2⌋). Using techniques that were originally developed for studying moments of L-functions over number fields, Andrade and Keating conjectured an asymptotic formula for the moments for q fixed and q→∞. We provide theoretical and numerical evidence in favour of their conjecture. In some cases, we are able to work out exact formulae for the moments and use these to precisely determine the size of the remainder term in the predicted moments. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

  6. Quantum-mechanical tunneling differential operators, zeta-functions and determinants

    Casahorrán, J


    We consider in detail the quantum-mechanical problem associated with the motion of a one-dimensional particle under the action of the double-well potential. Our main tool will be the euclidean (imaginary time) version of the path-integral method. Once we perform the Wick rotation, the euclidean equation of motion is the same as the usual one for the point particle in real time, except that the potential at issue is turned upside down. In doing so, our double-well potential becomes a two-humped potential. As required by the semiclassical approximation we may study the quadratic fluctuations over the instanton which represents in this context the localised finite-action solutions of the euclidean equation of motion. The determinants of the quadratic differential operators are evaluated by means of the zeta-function method. We write in closed form the eigenfunctions as well as the energy eigenvalues corresponding to such operators by using the shape-invariance symmetry. The effect of the multi-instantons configu...

  7. Phenylalanine-induced leucopenia in genetic and dichloroacetic acid generated deficiency of glutathione transferase Zeta.

    Theodoratos, Angelo; Tu, Wen Juan; Cappello, Jean; Blackburn, Anneke C; Matthaei, Klaus; Board, Philip G


    Glutathione transferase Zeta (GSTZ1-1) is identical to maleylacetoacetate isomerase and catalyses a significant step in the catabolism of phenylalanine and tyrosine. Exposure of GSTZ1-1 deficient mice to high dietary phenylalanine causes a rapid loss of circulating white blood cells (WBCs). The loss was significant (P<0.05) after 2 days and total WBCs were reduced by 60% after 6 days. The rapid loss of WBCs was attributed to the accumulation of the catabolic intermediates maleylacetoacetate or maleylacetone (MA) in the circulation. Serum from GSTZ1-1 deficient mice treated with phenylalanine was cytotoxic to splenocytes from normal BALB/c mice and direct incubation of normal splenocytes with MA caused a rapid loss of viability. Dichloroacetic acid (DCA) has been used therapeutically to treat lactic acidosis and is potentially of use in cancer chemotherapy. Since DCA can inactivate GSTZ1-1 there is a possibility that long-term treatment of patients with DCA could cause GSTZ1-1 deficiency and susceptibility to oxidative stress and phenylalanine/tyrosine-induced WBC loss. However, although we found that DCA at 200mg/(kg day) causes a severe loss of hepatic GSTZ1-1 activity in BALB/c mice, it did not induce WBC cytotoxicity when combined with high dietary phenylalanine.

  8. Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta.

    Gerlach, Gary F; Wingert, Rebecca A


    The zebrafish pronephros provides an excellent in vivo system to study the mechanisms of vertebrate nephron development. When and how renal progenitors in the zebrafish embryo undergo tubulogenesis to form nephrons is poorly understood, but is known to involve a mesenchymal to epithelial transition (MET) and the acquisition of polarity. Here, we determined the precise timing of these events in pronephros tubulogenesis. As the ternary polarity complex is an essential regulator of epithelial cell polarity across tissues, we performed gene knockdown studies to assess the roles of the related factors atypical protein kinase C iota and zeta (prkcι, prkcζ). We found that prkcι and prkcζ serve partially redundant functions to establish pronephros tubule epithelium polarity. Further, the loss of prkcι or the combined knockdown of prkcι/ζ disrupted proximal tubule morphogenesis and podocyte migration due to cardiac defects that prevented normal fluid flow to the kidney. Surprisingly, tubule cells in prkcι/ζ morphants displayed ectopic expression of the transcription factor pax2a and the podocyte-associated genes wt1a, wt1b, and podxl, suggesting that prkcι/ζ are needed to maintain renal epithelial identity. Knockdown of genes essential for cardiac contractility and vascular flow to the kidney, such as tnnt2a, or elimination of pronephros fluid output through knockdown of the intraflagellar transport gene ift88, was not associated with ectopic pronephros gene expression, thus suggesting a unique role for prkcι/ζ in maintaining tubule epithelial identity separate from the consequence of disruptions to renal fluid flow. Interestingly, knockdown of pax2a, but not wt1a, was sufficient to rescue ectopic tubule gene expression in prkcι/ζ morphants. These data suggest a model in which the redundant activities of prkcι and prkcζ are essential to establish tubule epithelial polarity and also serve to maintain proper epithelial cell type identity in the tubule by

  9. Can Eccentric Debris Disks Be Long-lived? A First Numerical Investigation and Application to Zeta(exp 2) Reticuli

    Faramaz, V.; Beust, H.; Thebault, P.; Augereau, J.-C.; Bonsor, A.; delBurgo, C.; Ertel, S.; Marshall, J. P.; Milli, J.; Montesinos, B.; Mora, A.; Bryden, G.; Danchi, William C.; Eiroa, C.; White, G. J.; Wolf, S.


    Context. Imaging of debris disks has found evidence for both eccentric and offset disks. One hypothesis is that they provide evidence for massive perturbers, for example, planets or binary companions, which sculpt the observed structures. One such disk was recently observed in the far-IR by the Herschel Space Observatory around Zeta2 Reticuli. In contrast with previously reported systems, the disk is significantly eccentric, and the system is several Gyr old. Aims. We aim to investigate the long-term evolution of eccentric structures in debris disks caused by a perturber on an eccentric orbit around the star. We hypothesise that the observed eccentric disk around Zeta2 Reticuli might be evidence of such a scenario. If so, we are able to constrain the mass and orbit of a potential perturber, either a giant planet or a binary companion. Methods. Analytical techniques were used to predict the effects of a perturber on a debris disk. Numerical N-body simulations were used to verify these results and further investigate the observable structures that may be produced by eccentric perturbers. The long-term evolution of the disk geometry was examined, with particular application to the Zeta2 Reticuli system. In addition, synthetic images of the disk were produced for direct comparison with Herschel observations. Results. We show that an eccentric companion can produce both the observed offsets and eccentric disks. These effects are not immediate, and we characterise the timescale required for the disk to develop to an eccentric state (and any spirals to vanish). For Zeta2 Reticuli, we derive limits on the mass and orbit of the companion required to produce the observations. Synthetic images show that the pattern observed around Zeta2 Reticuli can be produced by an eccentric disk seen close to edge-on, and allow us to bring additional constraints on the disk parameters of our model (disk flux and extent). Conclusions. We conclude that eccentric planets or stellar companions

  10. Visualizing the transient electroosmotic flow and measuring the zeta potential of microchannels with a micro-PIV technique.

    Yan, Deguang; Nguyen, Nam-Trung; Yang, Chun; Huang, Xiaoyang


    We have demonstrated a transient micro particle image velocimetry (micro-PIV) technique to measure the temporal development of electroosmotic flow in microchannels. Synchronization of different trigger signals for the laser, the CCD camera, and the high-voltage switch makes this measurement possible with a conventional micro-PIV setup. Using the transient micro-PIV technique, we have further proposed a method on the basis of inertial decoupling between the particle electrophoretic motion and the fluid electroosmotic flow to determine the electrophoretic component in the particle velocity and the zeta potential of the channel wall. It is shown that using the measured zeta potentials, the theoretical predictions agree well with the transient response of the electroosmotic velocities measured in this work.

  11. Influence of temperature, anions and size distribution on the zeta potential of DMPC, DPPC and DMPE lipid vesicles.

    Morini, M A; Sierra, M B; Pedroni, V I; Alarcon, L M; Appignanesi, G A; Disalvo, E A


    The purpose of the work is to compare the influence of the multilamellarity, phase state, lipid head groups and ionic media on the origin of the surface potential of lipid membranes. With this aim, we present a new analysis of the zeta potential of multilamellar and unilamellar vesicles composed by phosphatidylcholines (PC) and phosphatidylethanolamines (PE) dispersed in water and ionic solutions of polarizable anions, at temperatures below and above the phase transition. In general, the adsorption of anions seems to explain the origin of the zeta potential in vesicles only above the transition temperature (Tc). In this case, the sign of the surface potential is ascribed to a partial orientation of head group moiety toward the aqueous phase. This is noticeable in PC head groups but not in PEs, due to the strong lateral interaction between PO and NH group in PE.

  12. X-ray Observations of Bow Shocks around Runaway O Stars. The case of $\\zeta$ Oph and BD+433654

    Toalá, J A; González-Gaán, A; Guerrero, M A; Ignace, R; Pohl, M


    Non-thermal radiation has been predicted within bow shocks around runaway stars by recent theoretical works. We present X-ray observations towards the runaway stars $\\zeta$ Oph (Chandra and Suzaku) and BD+433654 (XMM-Newton) to search for the presence of non-thermal X-ray emission. We found no evidence of non-thermal emission spatially coincident with the bow shocks, nonetheless, diffuse emission is detected in the vicinity of $\\zeta$ Oph. After a careful analysis of its spectral characteristics we conclude that this emission has a thermal nature with a plasma temperature of $T \\approx 2 \\times10^{6}$ K. The cometary shape of this emission seems to be in line with recent predictions of radiation-hydrodynamic models of runaway stars. The case of BD+433654 is puzzling as non-thermal emission has been reported in a previous work for this source.

  13. Characterization of Ser73 in Arabidopsis thaliana Glutathione S-transferase zeta class


    Glutathione S-transferases (GSTs) are ubiquitous detoxifying superfamily enzymes. The zeta class GST from Arabidopsis thaliana (AtGSTZ) can efficiently degrade dichloroacetic acid (DCA), which is a common carcinogenic contaminant in drinking water. Ser73 in AtGSTZ is a conserved residue at Glutathione binding site (G-site). Compared with the equivalent residues in other GSTs, the catalytic and structural properties of Ser73 were poorly investigated. In this article, site-saturation mutagenesis was performed to characterize the detailed role of Ser73. The DCA de.chlorinating (DCA-DC) activity showed that most of the mutants had less than 3% of the wild-type activity, except S73T and $73A showing 43.48% and 21.62% of the wild-type activity, respectively, indicating that position 73 in AtGSTZ showed low mutational substitutability. Kinetic experiments revealed that mutants S73T, $73A, and S73G showed low binding affinity and catalytic efficiency toward DCA, 1.8-, 3.1-, and 10.7- fold increases in KmDcA values and 4.0-, 9.6-, and 34.1- fold decreases in KcatDCA/KmDCA values, respectively, compared to the wild type. Thermostability and refolding experiments showed that the wild type maintalned more thermostability and recovered activity. These results demonstrated the important role of Set73 in catalytic activity and structural stability of the enzyme. Such properties of Set73 could be particularly crucial to the molecular evolution of AtGSTZ and might,therefore, help explain why Ser73 is conserved in all GSTs. This conclusion might provide insights into the directed evolution of the DCA-DC activity of AtGSTZ.

  14. The Role of TSC2 Phosphorylation in the Regulation of TSC2 Localization and mTOR Signaling


    Madison, WI), and the University of Wisconsin School of Veterinary Medicine Phi Zeta Research Day (April 2015, Madison, WI). 4. IMPACT:  What was the...the Mechanical Regulation of mTOR Signaling. University of Wisconsin School of Veterinary Medicine Phi Zeta Research Day 2015. Madison, WI...mechanical stimuli regulate skeletal muscle mass.  What was the impact on technology transfer? Nothing to Report  What was the impact on society

  15. Correlations between the zeta potentials of silica hydride-based stationary phases, analyte retention behaviour and their ionic interaction descriptors.

    Kulsing, Chadin; Yang, Yuanzhong; Munera, Caesar; Tse, Colby; Matyska, Maria T; Pesek, Joseph J; Boysen, Reinhard I; Hearn, Milton T W


    In this study, the zeta potentials of type-B silica, bare silica hydride, the so-called Diamond Hydride™ and phenyl substituted silica hydride stationary phases have been measured in aqueous-organic media and correction procedures developed to account for the more negative zeta potential values in media containing different acetonitrile contents. Retention studies of 16 basic, acidic and neutral compounds were also performed with these four stationary phases with mobile phases containing 0.1% (v/v) formic acid and various acetonitrile-water compositions ranging from 0-90% (v/v) acetonitrile. The retention properties of these analytes were correlated to the corrected stationary phase zeta potentials measured under these different mobile phase conditions with R(2) values ranging from 0.01 to 1.00, depending on the stationary phase and analyte type. Using linear solvation energy relationships, stationary phase descriptors for each stationary phase have been developed for the different mobile phase conditions. Very high correlations of the zeta potentials with the ionic interaction descriptors were obtained for the type-B silica and the Diamond Hydride™ phases and good correlation with bare silica hydride material whilst there was no correlation observed for the phenyl substituted silica hydride phase. The nature of the retention mechanisms which gives rise to these different observations is discussed. The described methods represent a useful new approach to characterize and assess the retention properties of silica-hydride based chromatographic stationary phases of varying bonded-phase coverage and chemistries, as would be broadly applicable to other types of stationary phase used in the separation sciences.

  16. Weak magnetic field, solid-envelope rotation, and wave-induced N-enrichment in the SPB star $\\zeta$ Cassiopeiae

    Briquet, M; Petit, P; Leroy, B; de Batz, B


    Aims. The main-sequence B-type star $\\zeta$ Cassiopeiae is known as a N-rich star with a magnetic field discovered with the Musicos spectropolarimeter. We model the magnetic field of the star by means of 82 new spectropolarimetric observations of higher precision to investigate the field strength, topology, and effect. Methods. We gathered data with the Narval spectropolarimeter installed at T\\'elescope Bernard Lyot (TBL, Pic du Midi, France) and applied the least-squares deconvolution technique to measure the circular polarisation of the light emitted from $\\zeta$ Cas. We used a dipole oblique rotator model to determine the field configuration by fitting the longitudinal field measurements and by synthesizing the measured Stokes V profiles. We also made use of the Zeeman-Doppler Imaging technique to map the stellar surface and to deduce the difference in rotation rate between the pole and equator. Results. $\\zeta$ Cas exhibits a polar field strength $B_{\\rm pol}$ of 100-150 G, which is the weakest polar fiel...

  17. Zeta potential in oil-water-carbonate systems and its impact on oil recovery during controlled salinity water-flooding

    Jackson, Matthew D.; Al-Mahrouqi, Dawoud; Vinogradov, Jan


    Laboratory experiments and field trials have shown that oil recovery from carbonate reservoirs can be increased by modifying the brine composition injected during recovery in a process termed controlled salinity water-flooding (CSW). However, CSW remains poorly understood and there is no method to predict the optimum CSW composition. This work demonstrates for the first time that improved oil recovery (IOR) during CSW is strongly correlated to changes in zeta potential at both the mineral-water and oil-water interfaces. We report experiments in which IOR during CSW occurs only when the change in brine composition induces a repulsive electrostatic force between the oil-brine and mineral-brine interfaces. The polarity of the zeta potential at both interfaces must be determined when designing the optimum CSW composition. A new experimental method is presented that allows this. Results also show for the first time that the zeta potential at the oil-water interface may be positive at conditions relevant to carbonate reservoirs. A key challenge for any model of CSW is to explain why IOR is not always observed. Here we suggest that failures using the conventional (dilution) approach to CSW may have been caused by a positively charged oil-water interface that had not been identified.

  18. Epoetin zeta in the management of anemia associated with chronic kidney disease, differential pharmacology and clinical utility

    Davis-Ajami ML


    Full Text Available Mary Lynn Davis-Ajami,1 Jun Wu,2 Katherine Downton,3 Emilie Ludeman,3 Virginia Noxon4 1Organizational Systems and Adult Health, University of Maryland School of Nursing, Baltimore, MD, USA; 2South Carolina College of Pharmacy, University of South Carolina, Greenville, SC, USA; 3Health Sciences and Human Services Library, University of Maryland, Baltimore, MD, USA; 4Department of Clinical Pharmacy and Outcomes Science, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC, USA Abstract: Epoetin zeta was granted marketing authorization in October 2007 by the European Medicines Agency as a recombinant human erythropoietin erythropoiesis-stimulating agent to treat symptomatic anemia of renal origin in adult and pediatric patients on hemodialysis and adults on peritoneal dialysis, as well as for symptomatic renal anemia in adult patients with renal insufficiency not yet on dialysis. Currently, epoetin zeta can be administered either subcutaneously or intravenously to correct for hemoglobin concentrations ≤10 g/dL (6.2 mmol/L or with dose adjustment to maintain hemoglobin levels at desired levels not in excess of 12 g/dL (7.5 mmol/L. This review article focuses on epoetin zeta indications in chronic kidney disease, its use in managing anemia of renal origin, and discusses its pharmacology and clinical utility. Keywords: biosimilar, chronic kidney disease, epoetin alfa, erythropoiesis, renal anemia, Retacrit®

  19. A maternally inherited autosomal point mutation in human phospholipase C zeta (PLCζ) leads to male infertility

    Kashir, Junaid; Konstantinidis, Michalis; Jones, Celine; Lemmon, Bernadette; Chang Lee, Hoi; Hamer, Rebecca; Heindryckx, Bjorn; Deane, Charlotte M; De Sutter, Petra; Fissore, Rafael A; Parrington, John; Wells, Dagan; Coward, Kevin


    .... Certain types of male infertility are caused by failure of the sperm to activate the oocyte, a process normally regulated by calcium oscillations, thought to be induced by a sperm-specific phospholipase C, PLCzeta (PLCζ...

  20. Protein adsorption on dopamine-melanin films: role of electrostatic interactions inferred from zeta-potential measurements versus chemisorption.

    Bernsmann, Falk; Frisch, Benoît; Ringwald, Christian; Ball, Vincent


    We recently showed the possibility to build dopamine-melanin films of controlled thickness by successive immersions of a substrate in alkaline solutions of dopamine [F. Bernsmann, A. Ponche, C. Ringwald, J. Hemmerlé, J. Raya, B. Bechinger, J.-C. Voegel, P. Schaaf, V. Ball, J. Phys. Chem. C 113 (2009) 8234-8242]. In this work the structure and properties of such films are further explored. The zeta-potential of dopamine-melanin films is measured as a function of the total immersion time to build the film. It appears that the film bears a constant zeta-potential of (-39+/-3) mV after 12 immersion steps. These data are used to calculate the surface density of charged groups of the dopamine-melanin films at pH 8.5 that are mostly catechol or quinone imine chemical groups. Furthermore the zeta-potential is used to explain the adsorption of three model proteins (lysozyme, myoglobin, alpha-lactalbumin), which is monitored by quartz crystal microbalance. We come to the conclusion that protein adsorption on dopamine-melanin is not only determined by possible covalent binding between amino groups of the proteins and catechol groups of dopamine-melanin but that electrostatic interactions contribute to protein binding. Part of the adsorbed proteins can be desorbed by sodium dodecylsulfate solutions at the critical micellar concentration. The fraction of weakly bound proteins decreases with their isoelectric point. Additionally the number of available sites for covalent binding of amino groups on melanin grains is quantified.

  1. The Evolution of Los Zetas in Mexico and Central America: Sadism as an Instrument of Cartel Warfare


    96. “Cae operadora financiera del crimen organizado ” (“Fi- nancial Operator of Organized Crime Captured”), Diario de Xa- lapa, October 28, 2011. 97...jefe de plaza en Saltillo” (“Zeta Plaza Boss in Saltillo Captured with 13 Federal Police”), Excélsior, November 24, 2013; and “Los Golpes al crimen ...Likely Behind New Prison Break,” Border Beat, September 19, 2012; “Entra crimen obra pública” (“Crime Enters Public Works”), Reforma, Oc- tober 14

  2. On the Distribution of the Zeros of the Riemann Zeta-Function and Existence of Large Gaps

    Saker, S H


    In this paper, we prove a new Wirtinger-type inequality and assuming that the Riemann hypothesis is true we establish a new explicit formula for the gaps between the zeros of the Riemann zeta-function. On the hypothesis that the moments of the Hardy Z-function and its derivatives are correctly predicted we establish new lower bounds for the gaps between the zeros. In particular it is proved that consecutive nontrivial zeros often differ by at least 11.249 times the average spacing.

  3. Factors that can interfere with virus concentration from wastewater when using zeta plus 60S filter membranes

    Queiroz APS


    Full Text Available Zeta plus filter membranes (ZP60S have been shown to be efficient for rotavirus concentration from wastewater and for the reduction of cytotoxicity for cell cultures. Recently a variability in both properties was observed. In view of the low costs and the high virus recovery rates obtained in the past, we re-evaluated the application of ZP60S filter membranes for virus concentration from environmental samples. Some factors that could interfere with the concentration strategy using ZP60S were also considered and assessed including the type of water to be filtered and the possible release of toxic substances from the membrane matrix during filtration.

  4. Quasi-SMILES and nano-QFPR: The predictive model for zeta potentials of metal oxide nanoparticles

    Toropov, Andrey A.; Achary, P. Ganga Raju; Toropova, Alla P.


    Building up of the predictive quantitative structure-property/activity relationships (QSPRs/QSARs) for nanomaterials usually are impossible owing to the complexity of the molecular architecture of the nanomaterials. Simplified molecular input-line entry system (SMILES) is a tool to represent the molecular architecture of ;traditional; molecules for "traditional" QSPR/QSAR. The quasi-SMILES is a tool to represent features (conditions and circumstances), which accompany the behavior of nanomaterials. Having, the training set and validation set, so-called quantitative feature-property relationships (QFPRs), based on the quasi-SMILES, one can build up model for zeta potentials of metal oxide nanoparticles for situations characterized by different features.

  5. Prediction of the zeta potentials and ionic descriptors of a silica hydride stationary phase with mobile phases of different pH and ionic strength.

    Kulsing, Chadin; Yang, Yuanzhong; Matyska, Maria T; Pesek, Joseph J; Boysen, Reinhard I; Hearn, Milton T W


    In this study, the zeta potentials of a silica hydride stationary phase (Diamond Hydride™) in the presence of different water-acetonitrile mixtures (from 0-80% (v/v) acetonitrile) of different ionic strengths (from 0-40mM) and pH values (from pH 3.0-7.0) have been investigated. Debye-Hückel theory was applied to explain the effect of changes in the pH and ionic strength of these aqueous media on the negative zeta potential of this stationary phase. The experimental zeta potentials of the Diamond Hydride™ particles as a function of acetonitrile content up to 50% (v/v) correlated (R(2)=0.998) with the predicted zeta potential values based on this established theory, when the values of the dissociation constant of all related species, as well as viscosity, dielectric constant and refractive index of the aqueous medium were taken into consideration. Further, the retention behavior of basic, acidic and neutral analytes was investigated under mobile phase conditions of higher pH and lower ionic strength. Under these conditions, the Diamond Hydride™ stationary phase surface became more negative, as assessed from the increasingly more negative zeta potentials, resulting in the ion exchange characteristics becoming more dominant and the basic analytes showing increasing retention. Ionic descriptors were derived from these chromatographic experiments based on the assumption that linear solvation energy relationships prevail. The results were compared with predicted ionic descriptors based on the different calculated zeta potential values resulting in an overall correlation of R(2)=0.888. These studies provide fundamental insights into the impact on the separation performance of changes in the zeta potential of the Diamond Hydride™ surface with the results relevant to other silica hydride and, potentially, to other types of stationary phase materials.

  6. Zeta Sperm Selection Improves Pregnancy Rate and Alters Sex Ratio in Male Factor Infertility Patients: A Double-Blind, Randomized Clinical Trial

    Nasr Esfahani Mohammad Hossein


    Full Text Available Background Selection of sperm for intra-cytoplasmic sperm injection (ICSI is usually considered as the ultimate technique to alleviate male-factor infertility. In routine ICSI, selection is based on morphology and viability which does not necessarily preclude the chance injection of DNA-damaged or apoptotic sperm into the oocyte. Sperm with high negative surface electrical charge, named “Zeta potential”, are mature and more likely to have intact chromatin. In addition, X-bearing spermatozoa carry more negative charge. Therefore, we aimed to compare the clinical outcomes of Zeta procedure with routine sperm selection in infertile men candidate for ICSI. Materials and Methods From a total of 203 ICSI cycles studied, 101 cycles were allocated to density gradient centrifugation (DGC/Zeta group and the remaining 102 were included in the DGC group in this prospective study. Clinical outcomes were com- pared between the two groups. The ratios of Xand Y bearing sperm were assessed by fluorescence in situ hybridization (FISH and quantitative polymerase chain reaction (qPCR methods in 17 independent semen samples. Results In the present double-blind randomized clinical trial, a significant increase in top quality embryos and pregnancy rate were observed in DGC/Zeta group compared to DGC group. Moreover, sex ratio (XY/XX at birth significantly was lower in the DGC/Zeta group compared to DGC group despite similar ratio of X/Y bearings sper- matozoa following Zeta selection. Conclusion Zeta method not only improves the percentage of top embryo quality and pregnancy outcome but also alters the sex ratio compared to the conventional DGC method, despite no significant change in the ratio of Xand Ybearing sperm population (Registration number: IRCT201108047223N1.

  7. A detailed X-ray investigation of {\\zeta} Puppis III. A spectral analysis of the whole RGS spectrum

    Hervé, A; Nazé, Y


    Context. Zeta Pup is the X-ray brightest O-type star of the sky. This object was regularly observed with the RGS instrument aboard XMM-Newton for calibration purposes, leading to an unprecedented set of high-quality spectra. Aims. We have previously reduced and extracted this data set and combined it into the most detailed high-resolution X-ray spectrum of any early-type star so far. Here we present the analysis of this spectrum accounting for the presence of structures in the stellar wind. Methods. For this purpose, we use our new modeling tool that allows fitting the entire spectrum with a multi-temperature plasma. We illustrate the impact of a proper treatment of the radial dependence of the X-ray opacity of the cool wind on the best-fit radial distribution of the temperature of the X-ray plasma. Results. The best fit of the RGS spectrum of Zeta Pup is obtained assuming no porosity. Four plasma components at temperatures between 0.10 and 0.69 keV are needed to adequately represent the observed spectrum. Wh...

  8. On accuracy of mathematical languages used to deal with the Riemann zeta function and the Dirichlet eta function

    Sergeyev, Yaroslav D


    The Riemann Hypothesis has been of central interest to mathematicians for a long time and many unsuccessful attempts have been made to either prove or disprove it. Since the Riemann zeta function is defined as a sum of the infinite number of items, in this paper, we look at the Riemann Hypothesis using a new applied approach to infinity allowing one to easily execute numerical computations with various infinite and infinitesimal numbers in accordance with the principle `The part is less than the whole' observed in the physical world around us. The new approach allows one to work with functions and derivatives that can assume not only finite but also infinite and infinitesimal values and this possibility is used to study properties of the Riemann zeta function and the Dirichlet eta function. A new computational approach allowing one to evaluate these functions at certain points is proposed. Numerical examples are given. It is emphasized that different mathematical languages can be used to describe mathematical...

  9. Copernicus studies of interstellar material in the Perseus II complex. III - The line of sight to Zeta Persei

    Snow, T. P., Jr.


    Ultraviolet spectrophotometric data obtained with Copernicus are used to analyze the distribution, composition, density, temperature, and kinematics of the interstellar material along the line of sight to Zeta Persei. The far-UV extinction curve for the star is evaluated along with the kinematics of the interstellar gas, observations of atomic and molecular hydrogen, curves of growth for neutral and ionized species, atomic abundances and depletions, ionization equilibria, and observations of CO and OH lines. The results show that there are apparently three clouds along the line of sight to Zeta Persei: a main cloud at approximately +13 km/s which contains most of the material and forms all the neutral and molecular lines as well as most of the ionic lines, a second component at +22 km/s which must contribute to the strong UV lines of most ions, and a third component at roughly +2 km/s which gives rise to a strong Si III line at 1206 A. It is also found that the UV extinction curve has a somewhat steep far-UV rise, indicating the presence of a substantial number of small grains, and that about 30% of the hydrogen nuclei over the entire line of sight are in molecular form.

  10. The zeta potential and surface properties of asphaltenes obtained with different crude oil/n-heptane proportions

    Hilda Parra-Barraza; Daniel Hernandez-Montiel; Jaime Lizardi; Javier Hernandez; Ronaldo Herrera Urbina; Miguel A. Valdez [Universidad de Sonora, Sonora (Mexico). Departamento de Ciencias Quimico Biologicas


    We have investigated some surface properties of asphaltenes precipitated from crude oil with different volumes of n-heptane. According to the crude oil/n-heptane proportions used, asphaltenes are identified as 1:5, 1:15 and 1:40. Zeta potential results indicate that the amount of n-heptane determines the electrokinetic behaviour of asphaltenes in aqueous suspensions. Asphaltene 1:5 exhibits an isoelectric point (IEP) at pH 4.5 whereas asphaltenes 1:15 and 1:40 show an IEP at about pH 3. Surface charge on asphaltenes arises from the dissociation of acid functionalities and the protonation of basic functional groups. The presence of resins remaining on the asphaltene molecules may be responsible for the different IEP of asphaltene 1:5. Both sodium dodecyl sulfate (an anionic surfactant) and cetylpyridinium chloride (a cationic surfactant) were found to adsorb specifically onto asphaltenes. They reverse the sign of the zeta potential under certain conditions. These surfactants may be potential candidates to aid in controlling the stability of crude oil dispersions. Critical micelle concentration, interfacial tension measurements, and Langmuir isotherms at the air water interface confirm the different nature of asphaltene 1:5, which also showed more solubility and a larger molecular size. 26 refs., 3 figs., 1 tab.

  11. Effect of mutations mimicking phosphorylation on the structure and properties of human 14-3-3zeta.

    Sluchanko, Nikolai N; Chernik, Ivan S; Seit-Nebi, Alim S; Pivovarova, Anastasia V; Levitsky, Dmitrii I; Gusev, Nikolai B


    Effect of mutations mimicking phosphorylation on the structure of human 14-3-3zeta protein was analyzed by different methods. Mutation S58E increased intrinsic Trp fluorescence and binding of bis-ANS to 14-3-3. At low protein concentration mutation S58E increased the probability of dissociation of dimeric 14-3-3 and its susceptibility to proteolysis. Mutation S184E slightly increased Stokes radius and thermal stability of 14-3-3. Mutation T232E induced only small increase of Stokes radius and sedimentation coefficient that probably reflect the changes in the size or shape of 14-3-3. At low protein concentration the triple mutant S58E/S184E/T232E tended to dissociate, whereas at high concentration its properties were comparable with those of the wild type protein. The triple mutant was highly susceptible to proteolysis. Thus, mutation mimicking phosphorylation of Ser58 destabilized, whereas mutation of Ser184 induced stabilization of 14-3-3zeta structure.

  12. The Isolation of DNA by Polycharged Magnetic Particles: An Analysis of the Interaction by Zeta Potential and Particle Size

    Yazan Haddad


    Full Text Available Magnetic isolation of biological targets is in major demand in the biotechnology industry today. This study considers the interaction of four surface-modified magnetic micro- and nanoparticles with selected DNA fragments. Different surface modifications of nanomaghemite precursors were investigated: MAN37 (silica-coated, MAN127 (polyvinylpyrrolidone-coated, MAN158 (phosphate-coated, and MAN164 (tripolyphosphate-coated. All particles were positive polycharged agglomerated monodispersed systems. Mean particle sizes were 0.48, 2.97, 2.93, and 3.67 μm for MAN37, MAN127, MAN164, and MAN158, respectively. DNA fragments exhibited negative zeta potential of −0.22 mV under binding conditions (high ionic strength, low pH, and dehydration. A decrease in zeta potential of particles upon exposure to DNA was observed with exception of MAN158 particles. The measured particle size of MAN164 particles increased by nearly twofold upon exposure to DNA. Quantitative PCR isolation of DNA with a high retrieval rate was observed by magnetic particles MAN127 and MAN164. Interaction between polycharged magnetic particles and DNA is mediated by various binding mechanisms such as hydrophobic and electrostatic interactions. Future development of DNA isolation technology requires an understanding of the physical and biochemical conditions of this process.

  13. The Isolation of DNA by Polycharged Magnetic Particles: An Analysis of the Interaction by Zeta Potential and Particle Size.

    Haddad, Yazan; Xhaxhiu, Kledi; Kopel, Pavel; Hynek, David; Zitka, Ondrej; Adam, Vojtech


    Magnetic isolation of biological targets is in major demand in the biotechnology industry today. This study considers the interaction of four surface-modified magnetic micro- and nanoparticles with selected DNA fragments. Different surface modifications of nanomaghemite precursors were investigated: MAN37 (silica-coated), MAN127 (polyvinylpyrrolidone-coated), MAN158 (phosphate-coated), and MAN164 (tripolyphosphate-coated). All particles were positive polycharged agglomerated monodispersed systems. Mean particle sizes were 0.48, 2.97, 2.93, and 3.67 μm for MAN37, MAN127, MAN164, and MAN158, respectively. DNA fragments exhibited negative zeta potential of -0.22 mV under binding conditions (high ionic strength, low pH, and dehydration). A decrease in zeta potential of particles upon exposure to DNA was observed with exception of MAN158 particles. The measured particle size of MAN164 particles increased by nearly twofold upon exposure to DNA. Quantitative PCR isolation of DNA with a high retrieval rate was observed by magnetic particles MAN127 and MAN164. Interaction between polycharged magnetic particles and DNA is mediated by various binding mechanisms such as hydrophobic and electrostatic interactions. Future development of DNA isolation technology requires an understanding of the physical and biochemical conditions of this process.

  14. Characterization of an Effective Cleaning Procedure for Aluminum Alloys: Surface Enhanced Raman Spectroscopy and Zeta Potential Analysis

    Cherepy, N J; Shen, T H; Esposito, A P; Tillotson, T M


    We have developed a cleaning procedure for aluminum alloys for effective minimization of surface-adsorbed sub-micron particles and non-volatile residue. The procedure consists of a phosphoric acid etch followed by an alkaline detergent wash. To better understand the mechanism whereby this procedure reduces surface contaminants, we characterized the aluminum surface as a function of cleaning step using Surface Enhanced Raman Spectroscopy (SERS). SERS indicates that phosphoric acid etching re-establishes a surface oxide of different characteristics, including deposition of phosphate and increased hydration, while the subsequent alkaline detergent wash appears to remove the phosphate and modify the new surface oxide, possibly leading to a more compact surface oxide. We also studied the zeta potential of <5 micron pure aluminum and aluminum alloy 6061-T6 particles to determine how surface electrostatics may be affected during the cleaning process. The particles show a decrease in the magnitude of their zeta potential in the presence of detergent, and this effect is most pronounced for particles that have been etched with phosphoric acid. This reduction in magnitude of the surface attractive potential is in agreement with our observation that the phosphoric acid etch followed by detergent wash results in a decrease in surface-adsorbed sub-micron particulates.

  15. Joule heating effects on electromagnetohydrodynamic flow through a peristaltically induced micro-channel with different zeta potential and wall slip

    Ranjit, N. K.; Shit, G. C.


    This paper aims to develop a mathematical model for magnetohydrodynamic flow of biofluids through a hydrophobic micro-channel with periodically contracting and expanding walls under the influence of an axially applied electric field. The velocity slip effects have been taken into account at the channel walls by employing different slip lengths due to hydrophobic gating. Different temperature jump factors have also been used to investigate the thermomechanical interactions at the fluid-solid interface. The electromagnetohydrodynamic flow in a microchannel is simplified under the framework of Debye-Hückel linearization approximation. We have derived the closed-form solutions for the linearized dimensionless boundary value problem under the assumptions of long wave length and low Reynolds number. The axial velocity, temperature, pressure distribution, stream function, wall shear stress and the Nusselt number have been appraised for diverse values of the parameters approaching into the problem. Our main focus is to determine the effects of different zeta potential on the axial velocity and temperature distribution under electromagnetic environment. This study puts forward an important observation that the different zeta potential plays an important role in controlling fluid velocity. The study further reveals that the temperature increases significantly with the Joule heating parameter and the Brinkman number (arises due to the dissipation of energy).

  16. Ground State Energy Calculations of Isoelectronic Series of He in Double-Zeta Approximation Using Coulomb Potential with Noninteger Indices

    GUSEINOV I.Israfil; AKSU Hüseyin


    @@ Using formulae for one-and two-electron integrals of Coulomb interaction potential fk(r)=r-k with non-integer indices k established by one of the authors with the help of complete orthonormal sets of Ψa-exponential-type orbitals(a=1,0,-1,-2,…),we perform the calculations for isoelectronic series of the He atom containing nuclear charges from 2 to 10,where k=1-μ(-1<μ<0).For this purpose we have used the dogble-zeta approximation,the configuration interaction and coupled-cluster methods employing the integer-n Slater-type orbitals as basis sets.It is demonstrated that the results of calculations obtained are better than the numerical Hartree-Fock values.

  17. Overexpression of DNA polymerase zeta reduces the mitochondrial mutability caused by pathological mutations in DNA polymerase gamma in yeast.

    Enrico Baruffini

    Full Text Available In yeast, DNA polymerase zeta (Rev3 and Rev7 and Rev1, involved in the error-prone translesion synthesis during replication of nuclear DNA, localize also in mitochondria. We show that overexpression of Rev3 reduced the mtDNA extended mutability caused by a subclass of pathological mutations in Mip1, the yeast mitochondrial DNA polymerase orthologous to human Pol gamma. This beneficial effect was synergistic with the effect achieved by increasing the dNTPs pools. Since overexpression of Rev3 is detrimental for nuclear DNA mutability, we constructed a mutant Rev3 isoform unable to migrate into the nucleus: its overexpression reduced mtDNA mutability without increasing the nuclear one.

  18. An Earth-grazing fireball from the Daytime $\\zeta$-Perseid shower observed over Spain on 10 June 2012

    Madiedo, José M; Castro-Tirado, Alberto J; Pastor, Sensi; Reyes, José A de los


    On 10 June 2012, an Earth-grazer meteor which lasted over 17 s with an absolute magnitude of -4.0 $\\pm$ 0.5 was observed over Spain. This work focuses on the analysis of this rare event which is, to our knowledge, the faintest Earth-grazing meteor reported in the scientific literature, but also the first one belonging to a meteor shower. Thus, the orbital parameters show that the parent meteoroid belonged to the Daytime $\\zeta$-Perseid meteoroid stream. According to our calculations, the meteor was produced by a meteoroid with an initial mass ranging between 115 and 1.5 kg. During its encounter with Earth, the particle travelled about 510 km in the atmosphere. Around 260 g were destroyed in the atmosphere during the luminous phase of the event as a consequence of the ablation process. The modified orbit of the remaining material, which left our planet with a fusion crust, is also calculated.

  19. An exact trace formula and zeta functions for an infinite quantum graph with a non-standard Weyl asymptotics

    Endres, Sebastian Egger né; Steiner, Frank


    We study a quantum Hamiltonian that is given by the (negative) Laplacian and an infinite chain of δ-like potentials with strength κ > 0 on the half line { R}_{\\ge 0} and which is equivalent to a one-parameter family of Laplacians on an infinite metric graph. This graph consists of an infinite chain of edges with the metric structure defined by assigning an interval In = [0, ln], n\\in { N}, to each edge with length l_n=\\frac{\\pi }{n}. We show that the one-parameter family of quantum graphs possesses a purely discrete and strictly positive spectrum for each κ > 0 and prove that the Dirichlet Laplacian is the limit of the one-parameter family in the strong resolvent sense. The spectrum of the resulting Dirichlet quantum graph is also purely discrete. The eigenvalues are given by λn = n2, n\\in { N}, with multiplicities d(n), where d(n) denotes the divisor function. We can thus relate the spectral problem of this infinite quantum graph to Dirichlet's famous divisor problem and infer the non-standard Weyl asymptotics {N}(\\lambda )=\\frac{\\sqrt{\\lambda }}{2}\\ln \\lambda +\\mathord {{O}}(\\sqrt{\\lambda }) for the eigenvalue counting function. Based on an exact trace formula, the Voronoï summation formula, we derive explicit formulae for the trace of the wave group, the heat kernel, the resolvent and for various spectral zeta functions. These results enable us to establish a well-defined (renormalized) secular equation and a Selberg-like zeta function defined in terms of the classical periodic orbits of the graph for which we derive an exact functional equation and prove that the analogue of the Riemann hypothesis is true.


    Naze, Yaeel; Gosset, Eric [GAPHE, Departement AGO, Universite de Liege, Allee du 6 Aout 17, Bat. B5C, B-4000 Liege (Belgium); Oskinova, Lidia M., E-mail: [Institute for Physics and Astronomy, University of Potsdam, D-14476 Potsdam (Germany)


    Stellar winds are a crucial component of massive stars, but their exact properties still remain uncertain. To shed some light on this subject, we have analyzed an exceptional set of X-ray observations of {zeta} Puppis, one of the closest and brightest massive stars. The sensitive light curves that were derived reveal two major results. On the one hand, a slow modulation of the X-ray flux (with a relative amplitude of up to 15% over 16 hr in the 0.3-4.0 keV band) is detected. Its characteristic timescale cannot be determined with precision, but amounts from one to several days. It could be related to corotating interaction regions, known to exist in {zeta} Puppis from UV observations. Hour-long changes, linked to flares or to the pulsation activity, are not observed in the last decade covered by the XMM observations; the 17 hr tentative period, previously reported in a ROSAT analysis, is not confirmed either and is thus transient, at best. On the other hand, short-term changes are surprisingly small (<1% relative amplitude for the total energy band). In fact, they are compatible solely with the presence of Poisson noise in the data. This surprisingly low level of short-term variability, in view of the embedded wind-shock origin, requires a very high fragmentation of the stellar wind, for both absorbing and emitting features (>10{sup 5} parcels, comparing with a two-dimensional wind model). This is the first time that constraints have been placed on the number of clumps in an O-type star wind and from X-ray observations.

  1. Stress-energy tensor correlators in N-dimensional hot flat spaces via the generalized zeta-function method

    Cho, H. T.; Hu, B. L.


    We calculate the expectation values of the stress-energy bitensor defined at two different spacetime points x, x‧ of a massless, minimally coupled scalar field with respect to a quantum state at finite temperature T in a flat N-dimensional spacetime by means of the generalized zeta-function method. These correlators, also known as the noise kernels, give the fluctuations of energy and momentum density of a quantum field which are essential for the investigation of the physical effects of negative energy density in certain spacetimes or quantum states. They also act as the sources of the Einstein-Langevin equations in stochastic gravity which one can solve for the dynamics of metric fluctuations as in spacetime foams. In terms of constitutions these correlators are one rung above (in the sense of the correlation—BBGKY or Schwinger-Dyson—hierarchies) the mean (vacuum and thermal expectation) values of the stress-energy tensor which drive the semiclassical Einstein equation in semiclassical gravity. The low- and the high-temperature expansions of these correlators are also given here: at low temperatures, the leading order temperature dependence goes like TN while at high temperatures they have a T2 dependence with the subleading terms exponentially suppressed by e-T. We also discuss the singular behavior of the correlators in the x‧ → x coincident limit as was done before for massless conformal quantum fields. This article is part of a special issue of Journal of Physics A: Mathematical and Theoretical in honour of Stuart Dowker’s 75th birthday devoted to ‘Applications of zeta functions and other spectral functions in mathematics and physics’.

  2. Assembly, intracellular processing, and expression at the cell surface of the human alpha beta T cell receptor/CD3 complex. Function of the CD3-zeta chain

    Geisler, C; Kuhlmann, J; Rubin, B


    The TCR/CD3 complex is a multimeric protein complex composed of a minimum of seven transmembrane chains (TCR alpha beta-CD3 gamma delta epsilon zeta 2). Whereas earlier studies have demonstrated that both the TCR-alpha and -beta chains are required for the cell surface expression of the TCR/CD3 c...... to form the heptameric complex (TCR alpha beta-CD3 gamma delta epsilon----TCR alpha beta-CD3 gamma delta epsilon 2); and 5) CD3-zeta is required for the export of the TCR/CD3 complex from the endoplasmic reticulum to the Golgi apparatus for subsequent processing....

  3. Interactions of surfactants with a water treatment protein from Moringa oleifera seeds in solution studied by zeta-potential and light scattering measurements.

    Kwaambwa, Habauka M; Rennie, Adrian R


    Protein extracted from Moringa oleifera (MO) seeds has been advocated as a cheap and environmental friendly alternative to ionic flocculants for water purification. However, the nature and mechanism of its interaction with particles in water, as well as with dissolved surface-active molecules, are not well understood. In this article, we report studies of the protein and its interaction with four surfactants using dynamic light scattering (DLS), zeta-potential and turbidity measurements. Zeta-potential measurements identified points of charge reversal and the turbidity and DLS measurements were used to characterize the microstructure and size of protein-surfactant complexes. From the points of charge reversal, it was estimated that 7 anions are required to neutralize the positive charges of each protein molecule at pH 7. For protein mixtures with sodium dodecyl sulfate and dodecyl di-acid sodium salt, the peak in turbidity corresponds to concentrations with a large change in zeta-potential. No turbidity was observed for protein mixtures with either the nonionic surfactant Triton X-100 or the zwitterionic surfactant N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate. Changes of pH in the range 4-10 have little effect on the zeta-potential, turbidity, and the hydrodynamic radius reflecting the high isoelectric point of the protein. Addition of small amounts of salt has little effect on the size of protein in solution. These results are discussed in the context of the use of the MO protein in water treatment.

  4. Diacylglycerol kinase zeta inhibits myocardial atrophy and restores cardiac dysfunction in streptozotocin-induced diabetes mellitus.

    Bilim, Olga; Takeishi, Yasuchika; Kitahara, Tatsuro; Arimoto, Takanori; Niizeki, Takeshi; Sasaki, Toshiki; Goto, Kaoru; Kubota, Isao


    Activation of the diacylglycerol (DAG)-protein kinase C (PKC) pathway has been implicated in the pathogenesis of a number of diabetic complications. Diacylglycerol kinase (DGK) converts DAG to phosphatidic acid and acts as an endogenous regulator of PKC activity. Akt/PKB is associated with a downstream insulin signaling, and PKCbeta attenuates insulin-stimulated Akt phosphorylation. We examined transgenic mice with cardiac-specific overexpression of DGKzeta (DGKzeta-TG) compared to wild type (WT) mice in streptozotocin-induced (STZ, 150 mg/kg) diabetic and nondiabetic conditions. After 8 weeks, decreases in heart weight and heart weight/body weight ratio in diabetic WT mice were inhibited in DGKzeta-TG mice. Echocardiography at 8 weeks after STZ-injection demonstrated that decreases in left ventricular end-diastolic diameter and fractional shortening observed in WT mice were attenuated in DGKzeta-TG mice. Thinning of the interventricular septum and the posterior wall in diabetic WT hearts were blocked in DGKzeta-TG mice. Reduction of transverse diameter of cardiomyocytes isolated from the left ventricle in diabetic WT mice was attenuated in DGKzeta-TG mice. Cardiac fibrosis was much less in diabetic DGKzeta-TG than in diabetic WT mice. Western blots showed translocation of PKCbeta and delta isoforms to membrane fraction and decreased Akt/PKB phosphorylation in diabetic WT mouse hearts. However in diabetic DGKzeta-TG mice, neither translocation of PKC nor changes Akt/PKB phosphorylation was observed. DGKzeta modulates intracellular signaling and improves the course of diabetic cardiomyopathy. These data may suggest that DGKzeta is a new therapeutic target to prevent or reverse diabetic cardiomyopathy.

  5. A maternally inherited autosomal point mutation in human phospholipase C zeta (PLCζ) leads to male infertility.

    Kashir, Junaid; Konstantinidis, Michalis; Jones, Celine; Lemmon, Bernadette; Lee, Hoi Chang; Hamer, Rebecca; Heindryckx, Bjorn; Deane, Charlotte M; De Sutter, Petra; Fissore, Rafael A; Parrington, John; Wells, Dagan; Coward, Kevin


    Male factor and idiopathic infertility contribute significantly to global infertility, with abnormal testicular gene expression considered to be a major cause. Certain types of male infertility are caused by failure of the sperm to activate the oocyte, a process normally regulated by calcium oscillations, thought to be induced by a sperm-specific phospholipase C, PLCzeta (PLCζ). Previously, we identified a point mutation in an infertile male resulting in the substitution of histidine for proline at position 398 of the protein sequence (PLCζ(H398P)), leading to abnormal PLCζ function and infertility. Here, using a combination of direct-sequencing and mini-sequencing of the PLCζ gene from the patient and his family, we report the identification of a second PLCζ mutation in the same patient resulting in a histidine to leucine substitution at position 233 (PLCζ(H233L)), which is predicted to disrupt local protein interactions in a manner similar to PLCζ(H398P) and was shown to exhibit abnormal calcium oscillatory ability following predictive 3D modelling and cRNA injection in mouse oocytes respectively. We show that PLCζ(H233L) and PLCζ(H398P) exist on distinct parental chromosomes, the former inherited from the patient's mother and the latter from his father. Neither mutation was detected utilizing custom-made single-nucleotide polymorphism assays in 100 fertile males and females, or 8 infertile males with characterized oocyte activation deficiency. Collectively, our findings provide further evidence regarding the importance of PLCζ at oocyte activation and forms of male infertility where this is deficient. Additionally, we show that the inheritance patterns underlying male infertility are more complex than previously thought and may involve maternal mechanisms.


    France, Kevin; Nell, Nicholas; Kane, Robert; Green, James C. [Center for Astrophysics and Space Astronomy, University of Colorado, 389 UCB, Boulder, CO 80309 (United States); Burgh, Eric B. [SOFIA/USRA, NASA Ames Research Center, M/S N232-12, Moffett Field, CA 94035 (United States); Beasley, Matthew, E-mail: [Planetary Resources, Inc., 93 S Jackson St 50680, Seattle, WA 98104-2818 (United States)


    We present the first science results from the Sub-orbital Local Interstellar Cloud Experiment (SLICE): moderate resolution 1020-1070 A spectroscopy of four sightlines through the local interstellar medium. High signal-to-noise (S/N) spectra of {eta} Uma, {alpha} Vir, {delta} Sco, and {zeta} Oph were obtained during a 2013 April 21 rocket flight. The SLICE observations constrain the density, molecular photoexcitation rates, and physical conditions present in the interstellar material toward {delta} Sco and {zeta} Oph. Our spectra indicate a factor of two lower total N(H{sub 2}) than previously reported for {delta} Sco, which we attribute to higher S/N and better scattered light control in the new SLICE observations. We find N(H{sub 2}) = 1.5 Multiplication-Sign 10{sup 19} cm{sup -2} on the {delta} Sco sightline, with kinetic and excitation temperatures of 67 and 529 K, respectively, and a cloud density of n{sub H} = 56 cm{sup -3}. Our observations of the bulk of the molecular sightline toward {zeta} Oph are consistent with previous measurements (N(H{sub 2}) Almost-Equal-To 3 Multiplication-Sign 10{sup 20} cm{sup -2} at T{sub 01}(H{sub 2}) = 66 K and T{sub exc} = 350 K). However, we detect significantly more rotationally excited H{sub 2} toward {zeta} Oph than previously observed. We infer a cloud density in the rotationally excited component of n{sub H} Almost-Equal-To 7600 cm{sup -3} and suggest that the increased column densities of excited H{sub 2} are a result of the ongoing interaction between {zeta} Oph and its environment; also manifest as the prominent mid-IR bowshock observed by WISE and the presence of vibrationally excited H{sub 2} molecules observed by the Hubble Space Telescope.

  7. Biosynthesis and characterization of cadmium sulfide nanoparticles – An emphasis of zeta potential behavior due to capping

    Sankhla, Aryan, E-mail: [Centre for Converging Technologies, University of Rajasthan, Jaipur, 302015 (India); Sharma, Rajeshwar; Yadav, Raghvendra Singh [Centre for Converging Technologies, University of Rajasthan, Jaipur, 302015 (India); Kashyap, Diwakar [Department of Biological Chemistry, Ariel University, Ariel, 40700 (Israel); Kothari, S.L. [Institute of Biotechnology, Amity University, Jaipur, 303002 (India); Kachhwaha, S. [Department of Botany, University of Rajasthan, Jaipur, 302004 (India)


    Biological approaches have been amongst the most promising protocols for synthesis of nanomaterials. In this study, Cadmium sulfide nanoparticles (CdS NPs) were synthesized by incubating their precursor salts with Escherichia coli and zeta potential (ζ-potential) measurement with varying pH was carried out to evaluate stability of the colloidal dispersion. Formation of CdS NPs was studied in synchrony with microbial growth. TEM analysis confirmed the uniform distribution of NPs. Average size (5 ± 0.4 nm) and electron diffraction pattern revealed polycrystalline cubic crystal phase of these nanoparticles. X-ray diffractogram ascertained the formation of CdS nanoparticles with phase formation and particle size distribution in accordance with the particle size obtained from TEM. Absorption edge of biosynthesized CdS NPs showed a blue shift at ∼400 nm in comparison to their bulk counterpart. A hump at 279 nm indicated presence of biomolecules in the solution in addition to the particles. FT-IR spectrum of capped CdS NPs showed peaks of protein. This confirms adsorption of protein molecules on nanoparticle surface. They act as a capping agent hence responsible for the stability of NPs. The enhanced stability of the particles was confirmed by Zeta potential analysis. The presence of charge on the surface of capped CdS NPs gave a detail understanding of dispersion mechanism and colloidal stability at the NP interface. This stability study of biosynthesized semiconductor nanoparticles utilizing microbial cells had not been done in the past by any research group providing an impetus for the same. Surface area of capped CdS NPs and bare CdS NPs were found to be 298 ± 2.65 m{sup 2}/g and 117 ± 2.41 m{sup 2}/g respectively. A possible mechanism is also proposed for the biosynthesis of CdS NPs. - Highlights: • Synthesis of CdS NPs utilizing reproducible molecular machinery viz. Escherichia coli biomass. • Uniform and Polydispersed NPs with high surface area

  8. Rheology and zeta potential of cement pastes containing calcined silt and ground granulated blast-furnace slag

    Safi, B.


    Full Text Available This study aimed to analyse the re-use of dam silt as a supplementary binder for self-compacting concrete (SCC. When burnt, silt becomes more reactive because the kaolin it contains is converted into metakaolin. Portland cement, calcined or burnt silt and ground granulated blast furnace slag were used in this research. Cement pastes were prepared with blends containing two or three of these materials. The replacement ratio for burnt silt in both cases was 10 % and 20 % by cement weight and the ratio for the slag was a constant 30 % by weight of the blend. Rheological and zeta potential tests were conducted to evaluate paste electrokinetics and rheological behaviour. The findings showed that burnt silt is apt for use as an addition to cement for SCC manufacture.

    En el presente trabajo se ha analizado la posibilidad de utilizar los lodos procedentes de embalses como adición en la fabricación del hormigón autocompactante (HAC. Con la calcinación, estos materiales se vuelven más reactivos debido a la transformación en metacaolín, del caolín que forma parte de su composición. Las materias primas empleadas en esta investigación son: cemento Pórtland, lodos de embalse calcinados y escorias granuladas de horno alto. Se prepararon pastas de cemento con mezclas que contenían dos o tres de estos materiales. El porcentaje de reemplazo de los lodos calcinados osciló entre el 10 y el 20 % en peso del cemento, mientras que el de la escoria fue del 30 % en peso de la mezcla. Se llevaron a cabo ensayos reológicos y de potencial zeta para evaluar el comportamiento electrocinético y reológico de las distintas pastas. De acuerdo con los resultados obtenidos, una vez calcinados, los lodos de embalse son aprovechables como adición al cemento con destino a la preparación de HAC.

  9. Roles of vimentin and 14-3-3 zeta/delta in the inhibitory effects of heparin on PC-3M cell proliferation and B16-F10-luc-G5 cells metastasis

    Yah PAN; Xue-jun LI; Li-jun ZHONG; Hong ZHOU; Xin WANG; Kui CHEN; Hao-peng YANG; Yilixiati XIAOKAITI; Aikebaier MAIMAITI; Ling JIANG


    Aim:To investigate the inhibitory effects of heparin on PC-3M cells proliferation in vitro and B16-F10-luc-G5 cells metastasis in Balb/c nude mice and identify the protein expression patterns to elucidate the action mechanism of heparin.Methods:Human prostate cancer PC-3M cells were incubated with heparin 0.5 to 125 μg/mL for 24 h.The proliferation of PC-3M ceils was assessed by MTS assay.BrdU incoporation and Ki67 expression were detected using a high content screening (HCS) assay.The cell cycle and apoptosis of PC-3M cells were tested by flow cytometry.B16-F10-luc-G5 cardinoma cells were injected into the lateral tail vein of 6-week old male Balb/c nude mice and heparin 30 mg/kg was administered iv 30 min before and 24 h after injection.The metasis of B16-F10-luc-G5 cells was detected by bioluminescence assay.Activated partial thromboplastin time (APTT) and hemorheological parameters were measured on d 14 after injection of B16-F10-luc-G5 carcinoma cells in Balb/c mice.The global protein changes in PC-3M cells and frozen lung tissues from mice burdened with B16-F10-luc-G5 cells were determined by 2-dimensional gel electrophoresis and image analysis.The protein expression of vimentin and 14-3-3 zeta/delta was measured by Western blot.The mRNA transcription of vimentin,transforming growth factor (TGF)-β,E-cadherin,and αv-integrin was measured by RT-PCR.Results:Heparin 25 and 125 μg/mL significantly inhibited the proliferation,arrested the cells in G1 phase,and suppressed BrdU incorporation and Ki67 expression in PC-3M cells compared with the model group.But it had no significant effect on apoptosis of PC-3M cells.Heparin 30 mg/kg markedly inhibits the metastasis of B16-F10-luc-G5 cells on day 8.Additionally,heparin administration maintained relatively normal red blood hematocrit but had no influence on APTT in nude mice burdened with B16-F10-luc-G5 cells.Thirty of down-regulated protein spots were identified after heparin treatment,many of which are related to

  10. The Composition of Interstellar Grains Toward Zeta Ophiuchi: Constraining the Elemental Budget Near the Diffuse-Dense Cloud Transition

    Poteet, Charles A; Draine, Bruce T


    We investigate the composition of interstellar grains along the line of sight toward Zeta Ophiuchi, a well-studied environment near the diffuse-dense cloud transition. A spectral decomposition analysis of the solid-state absorbers is performed using archival spectroscopic observations from the Spitzer Space Telescope and Infrared Space Observatory. We find strong evidence for the presence of sub-micron-sized amorphous silicate grains, principally comprised of olivine-like composition, with no convincing evidence of H2O ice mantles. However, tentative evidence for thick H2O ice mantles on large (a ~ 2.8 microns) grains is presented. Solid-state abundances of elemental Mg, Si, Fe, and O are inferred from our analysis and compared to standard reference abundances. We find that nearly all of elemental Mg and Si along the line of sight are present in amorphous silicate grains, while a substantial fraction of elemental Fe resides in compounds other than silicates. Moreover, we find that the total abundance of eleme...

  11. Cyclic variability of the circumstellar disk of the Be star zetaTau - I. Long-term monitoring observations

    Stefl, S; Carciofi, A C; LeBouquin, J B; Baade, D; Bjorkman, K S; Hesselbach, E; Hummel, C A; Okazaki, A T; Pollmann, E; Rantakyrö, F; Wisniewski, J P


    Emission lines formed in decretion disks of Be stars often undergo long-term cyclic variations, especially in the violet-to-red (V/R) ratio of their primary components. From observations of the bright Be-shell star zeta Tau, the possibly broadest and longest data set illustrating the prototype of this behaviour was compiled from our own and archival observations. It comprises optical and infrared spectra, broad-band polarimetry, and interferometric observations. From 3 V/R cycles between 1997 and 2008, a mean cycle length in H alpha of 1400-1430 days was derived. After each minimum in V/R, the shell absorption weakens and splits into two components, leading to 3 emission peaks. This phase makes the strongest contribution to the variability in cycle length. V/R curves of different lines are shifted in phase. Lines formed on average closer to the central star are ahead of the others. The shell absorption lines fall into 2 categories differing in line width, ionization/excitation potential, and variability of th...

  12. Finite temperature Casimir energy in closed rectangular cavities: a rigorous derivation based on a zeta function technique

    Lim, S C [Faculty of Engineering, Multimedia University, Jalan Multimedia, Cyberjaya, 63100, Selangor Darul Ehsan (Malaysia); Teo, L P [Faculty of Information Technology, Multimedia University, Jalan Multimedia, Cyberjaya, 63100, Selangor Darul Ehsan (Malaysia)


    We derive rigorously explicit formulae of the Casimir free energy at finite temperature for massless scalar field and electromagnetic field confined in a closed rectangular cavity with different boundary conditions by a zeta regularization method. We study both the low and high temperature expansions of the free energy. In each case, we write the free energy as a sum of a polynomial in temperature plus exponentially decay terms. We show that the free energy is always a decreasing function of temperature. In the cases of massless scalar field with the Dirichlet boundary condition and electromagnetic field, the zero temperature Casimir free energy might be positive. In each of these cases, there is a unique transition temperature (as a function of the side lengths of the cavity) where the Casimir energy changes from positive to negative. When the space dimension is equal to two and three, we show graphically the dependence of this transition temperature on the side lengths of the cavity. Finally we also show that we can obtain the results for a non-closed rectangular cavity by letting the size of some directions of a closed cavity go to infinity, and we find that these results agree with the usual integration prescription adopted by other authors.

  13. Dielectric Enhancement from Non-Insulating Particles with Ideally Polarized Interfaces and Zero $\\zeta$-Potential I: Exact Solution

    Qian, Jiang


    We solve exactly the dielectric response of a non-insulating sphere of radius $a$ suspended in symmetric, univalent electrolyte solution, with ideally-polarizable interface but without significant $\\zeta$-potential. We then use this solution to derive the dielectric response of a dilute random suspension of such spheres, with volume fraction $f\\ll1$, within the Maxwell-Garnett Effective Medium Approximation. Surprisingly, we discover a huge dielectric enhancement in this bare essential model of dielectric responses of solids in electrolyte solution: at low frequency $\\omega\\tau_D \\ll (\\lambda/a) / (\\sigma_w / \\sigma_s+1/2)$, the real part of the effective dielectric constant of the mixture is $1-(3f/2)+(9f/4)(a/\\lambda)$. Here $\\sigma_{w/s}$ is the conductivity of the electrolyte solution/solids, $\\lambda$ is the Debye screening length in the solution, $\\tau_D=\\lambda^2/D$ is the standard time scale of diffusion and $D$ is the ion diffusion coefficient. As $\\lambda$ is of the order nm even for dilute electrol...

  14. Perfluorinated poly(dimethylsiloxane) via the covalent attachment of perfluoroalkylsilanes on the oxidized surface: Effects on zeta-potential values

    Sun, Peiling; Horton, J. Hugh


    Poly(dimethylsiloxane) (PDMS) is a widely-used polymer in microfluidic devices due to its range of physical and chemical properties suitable for molding micron-sized features. However, its hydrophobicity also leads to some limitations: it poorly supports electro-osmotic flow, and can be incompatible with biomolecules and with many organic solvents. Surface modification is commonly used to vary PDMS surface properties to make it more suitable for specific microfluidic applications. Here, we report on the surface modification of PDMS using perfluoroalkane-triethoxysilanes, via the covalent attachment of triethoxysilane groups on plasma-oxidized PDMS. A device constructed from such fluorinated materials could be used for separating fluorous-tagged proteins or peptides. Modified PDMS were characterized using a range of surface analytical methods. In particular, zeta- (ζ-) potential values at the interfaces of both modified and unmodified PDMS and under varying pH conditions were measured, as ζ-potential is an essential parameter to support electroosmotic flow (EOF), a common pumping method in microfluidic devices. The results showed the length of fluorinated alkane chain has significant effect on the density of surface modifying species and topography following modification. In addition, the perfluorinated modification increases the magnitude of the ζ-potential at the PDMS interface when compared to that of native PDMS, increasing the electro-osmotic flow rate, over a wide pH range. The modified surface is resistant to the diffusion of PDMS oligomers that affects other PDMS surface modification processes.

  15. A search for weak ultraviolet interstellar absorption features in IUE spectra of Rho Ophiuchi and Zeta Ophiuchi

    Welty, D. E.; Thorburn, J. A.; Hobbs, L. M.; York, D. G.


    We have applied procedures designed to reduce substantially the nonrandom, so-called 'fixed-pattern' noise present in IUE spectra to archival long-wavelength high-dispersion spectra of Rho Ophiuchi and Zeta Ophiuchi. Substantial elimination of the fixed-pattern noise via flat fielding can yield 2sigma equivalent width limits of 5-10 mA from the sum of a small number (about less than 5) of well-exposed archival spectra, and increases confidence in the reality of any weak features found. Examination of complete long-wavelength (about 2200-3250 A) spectra of these two stars has revealed, in addition to many known strong absorption lines, several lines of Fe I and Si I which had not previously been reported, as well as a small number of possible unidentified lines. We also present substantially improved upper limits to the equivalent widths of a number of other weak lines; limits an order of magnitude smaller, now achievable with the HST GHRS, should produce detections of some of these.

  16. Defects in the Zeta Chain Expression (ζ in a Group of Patients with SLE, Scleroderma and Late-onset Arthritis, Colombia 2014

    Heber Siachoque-Montañez


    Full Text Available Introduction: Systemic Lupus Erythematosus (SLE, Scleroderma and late-onset arthritis are autoimmune inflammatory diseases (EIA characterized by autoantibody production and presence of abnormal T cells which generate defective immune response. The abnormal expression of key signaling molecules in the defective function of T-lymphocytes plays a significant role in the pathogenesis of autoimmune disease. The T-cells exhibit numerous abnormalities TCRζ1 signaling complex, these aberrations result in altered expression of cytokines and some biochemical events involved in the expression of surface molecules. Defects in the complex may be associated TCRζ to steroids used in autoimmune disease patients due to their powerful anti-inflammatory activity and immunosuppressive properties. The synthetic corticosteroids such as examethasone inhibit the transcriptional activity of some factors such as NFKB and AP-1, which regulate the synthesis of certain cytokines and could be involved in the TCRζ synthesis. Material and Methods: A case-control study, with a 1:1 ratio of cases and controls (13:13. Cases were patients with active autoimmune disease (6 patients with SLE, 5 patients with scleroderma and 2 patients with lateonset arthritis, who have not started treatment with corticosteroids. Controls were patients with no autoimmune disease. The diagnosis was made by the criteria established by the American College of Rheumatology for patients with SLE, scleroderma and late-onset arthritis. A 10 mL sample was obtained by venipuncture whole blood. Total RNA was extracted and RT-PCR was performed using a set of primers flanking a region of 138 base pairs involving exons 2, 3 and 4 of the ζ chain. Results: The values of Z chain amplification showed significant differences in patients with autoimmune disease (0.8214 } 0.1787, med = 0.7368 compared with the control group (0.9225 } 0.1272, med = 0.9830 (p = 0.045, Mann-Withney non-parametric one tailed exact

  17. Failure to synthesize the human T-cell CD3-zeta chain and its consequence for the T-cell receptor-CD3 complex expression

    Geisler, C; Kuhlmann, J; Plesner, T;


    The T-cell antigen receptor is composed of two variable chains (alpha and beta, termed TcR) which confer ligand specificity, and four constant chains (gamma, delta, epsilon, and zeta, collectively termed CD3) whose functions are not fully understood. To explore the role of the individual CD3...... components, the human T-cell tumour line Jurkat was chemically mutagenized followed by negative selection with F101.01 (a monoclonal antibody against the TcR-CD3 complex), and cloning. Growing clones were analysed for TcR-CD3 expression by immunofluorescence. One clone, J79, was found to express greatly...... the normal intracellular fate of the TcR-CD3 complex, and that the CD3-zeta is necessary for the intracellular transport and expression at the cell surface of the TcR-CD3 complex....

  18. Cold dust around nearby stars (DUNES). First results: A resolved exo-Kuiper belt around the solar-like star zeta^2 Ret

    Eiroa, C; Maldonado, J; González-García, B M; Rodmann, J; Heras, A M; Pilbratt, G L; Augereau, J -Ch; Mora, A; Montesinos, B; Ardila, D; Bryden, G; Liseau, R; Stapelfeldt, K; Launhardt, R; Solano, E; Bayo, A; Absil, O; Ar?evalo, M; Barrado, D; Beichmann, C; Danchi, W; del Burgo, C; Ertel, S; Fridlund, M; Fukagawa, M; Gutiérrez, R; Grün, E; Kamp, I; Krivov, A; Lebreton, J; Löhne, T; Lorente, R; Marshall, J; Martínez-Arnáiz, R; Meeus, G; Montes, D; Morbidelli, A; Müller, S; Mutschke, H; Nakagawa, T; Olofsson, G; Ribas, I; Roberge, A; Sanz-Forcada, J; Thébault, P; Walker, H; White, G J; Wolf, S


    We present the first far-IR observations of the solar-type stars delta Pav, HR 8501, 51 Peg and zeta^2 Ret, taken within the context of the DUNES Herschel Open Time Key Programme (OTKP). This project uses the PACS and SPIRE instruments with the objective of studying infrared excesses due to exo-Kuiper belts around nearby solar-type stars. The observed 100 um fluxes from delta Pav, HR 8501, and 51 Peg agree with the predicted photospheric fluxes, excluding debris disks brighter than Ldust/Lstar ~ 5 x 10^-7 (1 sigma level) around those stars. A flattened, disk-like structure with a semi-major axis of ~ 100 AU in size is detected around zeta^2 Ret. The resolved structure suggests the presence of an eccentric dust ring, which we interpret as an exo-Kuiper belt with Ldust/Lstar ~ 10^-5.

  19. Computation of surface electrical potentials of plant cell membranes . Correspondence To published zeta potentials from diverse plant sources

    Kinraide; Yermiyahu; Rytwo


    A Gouy-Chapman-Stern model has been developed for the computation of surface electrical potential (psi0) of plant cell membranes in response to ionic solutes. The present model is a modification of an earlier version developed to compute the sorption of ions by wheat (Triticum aestivum L. cv Scout 66) root plasma membranes. A single set of model parameters generates values for psi0 that correlate highly with published zeta potentials of protoplasts and plasma membrane vesicles from diverse plant sources. The model assumes ion binding to a negatively charged site (R- = 0.3074 &mgr;mol m-2) and to a neutral site (P0 = 2.4 &mgr;mol m-2) according to the reactions R- + IZ &rlharr; RIZ-1 and P0 + IZ &rlharr; PIZ, where IZ represents an ion of charge Z. Binding constants for the negative site are 21, 500 M-1 for H+, 20,000 M-1 for Al3+, 2,200 M-1 for La3+, 30 M-1 for Ca2+ and Mg2+, and 1 M-1 for Na+ and K+. Binding constants for the neutral site are 1/180 the value for binding to the negative site. Ion activities at the membrane surface, computed on the basis of psi0, appear to determine many aspects of plant-mineral interactions, including mineral nutrition and the induction and alleviation of mineral toxicities, according to previous and ongoing studies. A computer program with instructions for the computation of psi0, ion binding, ion concentrations, and ion activities at membrane surfaces may be requested from the authors.

  20. RNA secondary structures in a polymer-zeta model how foldings should be shaped for sparsification to establish a linear speedup.

    Jin, Emma Yu; Nebel, Markus E


    Various tools used to predict the secondary structure for a given RNA sequence are based on dynamic programming used to compute a conformation of minimum free energy. For structures without pseudoknots, a worst-case runtime proportional to n3, with n being the length of the sequence, results since a table of dimension n2 has to be filled in while a single entry gives rise to a linear computational effort. However, it was recently observed that reformulating the corresponding dynamic programming recursion together with the bookkeeping of potential folding alternatives (a technique called sparsification) may reduce the runtime to n2 on average, assuming that nucleotides of distance d form a hydrogen bond (i..e., are paired) with probability b/d(c) for some constants b > 0, c > 1. The latter is called the polymer-zeta model and plays a crucial role in speeding up the above mentioned algorithm. In this paper we discuss the application of the polymer-zeta property for the analysis of sparsification, showing that it must be applied conditionally on first and last positions to pair. Afterwards, we will investigate the combinatorics of RNA secondary structures assuming that the corresponding conditional probabilities behave according to a polymer-zeta probability model. We show that even if some of the structural parameters exhibit an almost realistic behavior on average, the expected shape of a folding in that model must be assumed to highly differ from those observed in nature. More precisely, we prove our polymer-zeta model to be appropriate for mRNA molecules but to fail in connection with almost every other family of RNA. Those findings explain the huge speedup of the dynamic programming algorithm observed empirically by Wexler et al. when applying sparsification in connection with mRNA data.

  1. Electroacoustic and ultrasonic attenuation measurements of droplet size and zeta-potential of alkane-in-water emulsions: effects of oil solubility and composition.

    Djerdjev, Alex M; Beattie, James K


    The effects of oil solubility and composition on the zeta potential and drop size of oil-in-water emulsions stabilised by sodium dodecyl sulfate (SDS) were studied by electroacoustics and ultrasonic attenuation. The zeta-potentials of toluene and alkane emulsions were found to decrease (be less negative) as the water solubility of the dispersed oil phase increased. The zeta-potentials also depended on the composition of mixed oils, becoming more negative with increasing mole fraction of an insoluble oil (hexadecane). As the water solubility of the dispersed oil phase increased, the conductance within the Stern layer relative to the diffuse layer (K/K) increased, which is interpreted as due to the displacement of the shear plane further into the diffuse layer. The shear plane was calculated to increase from approximately 0.50 nm at the insoluble oil-water interface (hexadecane) to approximately 2.5 nm at a soluble oil-water interface of toluene. The lowering of the zeta-potentials of the soluble oils is ascribed to the shift of the shear plane into the diffuse layer, resulting in a more diffuse interface. The total surface conductance of the mixed oils was related to the log of the oil solubility and decreased from approximately 7 x 10(-9) Omega(-1) to 3 x 10(-9) Omega(-1) with increasing oil solubility from hexadecane to toluene, respectively. The lower surface conductance at the soluble oil-water interface is attributed to a reduction in the dielectric constant of the water inside of the shear plane, caused by the presence of the soluble oil.

  2. 'Syncing' Up with the Quinn-Rand-Strogatz Constant: Hurwitz-ZetaFunctions in Non-Linear physics

    Durgin, Natalie J.; Garcia, Sofia M.; Flournoy, Tamara; Bailey,David H.


    This work extends the analytical and computationalinvestigation of the Quinn-Rand-Strogatz (QRS) constants from non-linearphysics. The QRS constants (c1, c2, ..., cN) are found in a Winfreeoscillator mean-field system used to examine the transition of coupledoscillators as they lose synchronization. The constants are part of anasymptotic expansion of a function related to the oscillatorsynchronization. Previous work used high-precision software packages toevaluate c1 to 42 decimal-digits, which made it possible to recognize andprove that c1 was the root of a certain Hurwitz-zeta function. Thisallowed a value of c2 to beconjectured in terms of c1. Therefore thereis interest in determining the exact values of these constants to highprecision in the hope that general relationships can be establishedbetween the constants and the zeta functions. Here, we compute the valuesof the higher order constants (c3, c4) to more than 42-digit precision byextending an algorithm developed by D.H. Bailey, J.M. Borwein and R.E.Crandall. Several methods for speeding up the computation are exploredand an alternate proof that c1 is the root of a Hurwitz-zeta function isattempted.

  3. H2 Excitation Structure on the Sightlines to delta Scorpius and zeta Ophiucus - First Results from the Sub-orbital Local Interstellar Cloud Experiment

    France, Kevin; Kane, Robert; Burgh, Eric B; Beasley, Matthew; Green, James C


    We present the first science results from the Sub-orbital Local Interstellar Cloud Experiment (SLICE): moderate resolution 1020-1070A spectroscopy of four sightlines through the local interstellar medium. High signal-to-noise (S/N) spectra of eta Uma, alpha Vir, delta Sco, and zeta Oph were obtained during a 21 April 2013 rocket flight. The SLICE observations constrain the density, molecular photoexcitation rates, and physical conditions present in the interstellar material towards delta Sco and zeta Oph. Our spectra indicate a factor of two lower total N(H2) than previously reported for delta Sco, which we attribute to higher S/N and better scattered light control in the new SLICE observations. We find N(H2) = 1.5 x 10^{19} cm^{-2} on the delta Sco sightline, with kinetic and excitation temperatures of 67 and 529 K, respectively, and a cloud density of n_{H} = 56 cm^{-3}. Our observations of the bulk of the molecular sightline toward zeta Oph are consistent with previous measurements (N(H2) ~ 3 x 10^{20} cm^...

  4. Investigations of the influence of vapour phase inhibitors on the surface charge of iron oxide particles by zeta-potential measurements

    Kuenzelmann, U.; Reinhard, G. (Technische Univ., Dresden (German Democratic Republic). Sektion Chemie); Jacobasch, H.J. (Akademie der Wissenschaften der DDR, Dresden. Inst. fuer Technologie der Fasern (German Democratic Republic))


    Adsorbability of the vapour phase inhibitor dicyclohexylammonium nitrite (Dichan) and its ionic constituents present in aqueous media were investigated by means of zeta-potential measurements in 10{sup -3} M KCl solution in dependence on the pH-value using particles of various iron oxides and oxide hydroxides. It was pointed out, that the interactions between Dichan and iron oxides result in a negativation of the zeta-potential and in a displacement of the isoelectric point of the particles surface (IEPS) due to specific adsorption of anions. By dicyclohexylamine in the primary solution, the zeta-potential of iron oxides is hardly influenced, even when the saturation concentration is established. As nitrite changes the chemical surface properties when concentrations considerably higher than those indicated for Dichan are achieved, a synergism is assumed to be present because of the Dichan's high adsorbability at oxidic iron compounds. This effect probable consist in the promotion of the specific adsorption of nitrite ions at the oxide surface by the amine component. (orig.).

  5. An Euler-type formula for the Dirichlet beta function at even values and an exact closed-form expression for a class of rational zeta series

    Lima, F M S


    In a recent work [JNT \\textbf{118}, 192 (2006)], Dancs and He found an Euler-type formula for $ \\zeta{(2 n+1)}$, $ n $ being a positive integer, which contains an alternating series that seems not to be reducible to a finite closed-form. This certainly reflects a greater complexity in comparison to $\\zeta(2n)$, which is a rational multiple of $\\pi^{2n}$ according to a well-known formula by Euler. For the Dirichlet beta function, the things are "inverse": $\\beta(2n+1)$ is a rational multiple of $\\pi^{2n+1}$, whereas no closed-form expression is known for the numbers $\\beta(2n)$. Here in this work, I use the Dancs-He strategy for deriving an Euler-type formula for the Dirichlet beta function at even values of the argument, including $\\beta{(2)}$, i.e. the Catalan's constant. This yields a new series representation for these numbers. Finally, by converting the summand of these series into even zeta values and then making use of a formula by Milgran, I derive an exact closed-form expression for an important class...

  6. Interaction between ropinirole hydrochloride and aspirin with human serum albumin as binary and ternary systems by multi-spectroscopic, molecular modeling and zeta potential

    Mahaki, Hanie, E-mail: [Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad (Iran, Islamic Republic of); Memarpoor-Yazdi, Mina; Chamani, Jamshidkhan [Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad (Iran, Islamic Republic of); Reza Saberi, Mohammad [Medical Chemistry Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad (Iran, Islamic Republic of)


    The aim of the present study was to describe the competition of ropinirole hydrochloride (RP) and aspirin (ASA) in binding to human serum albumin (HSA) in physiological buffer (pH=7.4) using multi-spectroscopic, molecular modeling and zeta-potential measurements. Fluorescence analysis was used to define the binding and quenching properties of drug-HSA complexes in binary and ternary systems. Fluorescence spectroscopy showed that in the presence of RP, the binding constant of HSA-ASA was increased. Static quenching was confirmed to result in the fluorescence quenching and FRET. The effect of drugs on the conformation of HSA was analyzed using synchronous fluorescence spectroscopy, three-dimensional fluorescence spectra and circular dichroism (CD). The RLS method determined the critical aggregation concentration of drugs on HSA in binary and ternary systems that confirmed the zeta potential results. Structural modeling showed that the affinity of each of the drugs to HSA in binary and ternary systems confirms the spectroscopic results. - Highlights: Black-Right-Pointing-Pointer We studied the interaction of ropinirole hydrochloride and aspirin with HSA. Black-Right-Pointing-Pointer Molecular modeling and zeta-potential used to describe competitive interaction. Black-Right-Pointing-Pointer We determined the critical induced aggregation concentration of both drugs on HSA. Black-Right-Pointing-Pointer The binding mechanism of drugs as separate and simultaneous to HSA has been compared. Black-Right-Pointing-Pointer The binding site of both drugs as simultaneous effects on HSA has been determined.

  7. Zeta-Proteobacteria dominate the formation of microbial mats in low-temperature hydrothermal vents at Loihi Seamount

    Rassa, A. C.; McAllister, S. M.; Safran, S. A.; Moyer, C. L.


    Loihi Seamount is Hawaii's youngest volcano and one of the earth's most active. Loihi is located 30 km SE of the big island of Hawaii and rises over 3000m above the sea floor and summits at 1100m below sea level. An eruption in 1996 of Loihi led to the formation of Pele's Pit, a 300 meter deep caldera. The current observations have revealed diffuse hydrothermal venting causing low to intermediate temperatures (10 to 65°C). The elevated temperatures, coupled with high concentrations of Fe(II) (ranging from 50 to 750 μM) support conditions allowing for extensive microbial mat formation. The focus of this study was to identify the colonizing populations of bacteria generated by the microbial mats at Loihi Seamount. Twenty-six microbial growth chambers were deployed and recovered after placement in the flow of hydrothermal vents for 3 to 8 days from within Loihi's caldera. Genomic DNA was extracted from samples and analyzed by Terminal-Restriction Fragment Length Polymorphism (T-RFLP) using eight restriction enzyme treatments to generate fingerprints from bacterial amplicons of small subunit rRNA genes (SSU rDNAs). Pearson product-moment coupled with UPGMA cluster analysis of these T-RFLP fingerprints showed that these communities bifurcated into two primary clusters. The first (Group 1) had an average vent effluent temperature of 44°C, and the second (Group 2) had an average vent effluent temperature of 64°C. Representative samples from within the two clusters (or groups) were chosen for further clone library and sequencing analysis. These libraries revealing a dominance of the recently discovered zeta- Proteobacteria in the lower temperature group (Group 1) indicating that they were the dominant colonizers of the microbial mats. These microaerophilic, obligately lithotrophic, Fe-oxidizing bacteria are most closely related to Mariprofundus ferrooxydans. The higher temperature group (Group 2) was dominated by epsilon- Proteobacteria primarily of the genus

  8. HST-WFC3 Near-Infrared Spectroscopy of Quenched Galaxies at zeta approx 1.5 from the WISP Survey: Stellar Populations Properties

    Bedregal, A. G.; Scarlata, C.; Henry, A. L.; Atek, H.; Rafelski, M.; Teplitz, H. I.; Dominguez, A.; Siana, B.; Colbert, J. W.; Malkan, M.; Ross, N. R.; Martin, C. L.; Dressler, A.; Bridge, C.; Hathi, N. P.; Masters, D.; McCarthy, P. J.; Rutkowski, M. J.


    We combine Hubble Space Telescope (HST) G102 and G141 near-IR (NIR) grism spectroscopy with HST/WFC3- UVIS, HST/WFC3-IR, and Spitzer/IRAC [3.6 microns] photometry to assemble a sample of massive (log(Mstar/M solar mass) at approx 11.0) and quenched (specific star formation rate population properties. We find that their spectral energy distributions (SEDs) are well fitted with exponentially decreasing star formation histories and short star formation timescales (tau less than or equal to 100 M/yr). Quenched galaxies also show a wide distribution in ages, between 1 and 4 G/yr. In the (u - r)0-versus-mass space quenched galaxies have a large spread in rest-frame color at a given mass. Most quenched galaxies populate the zeta appro. 1.5 red sequence (RS), but an important fraction of them (32%) have substantially bluer colors. Although with a large spread, we find that the quenched galaxies on the RS have older median ages (3.1 G/yr) than the quenched galaxies off the RS (1.5 G/yr). We also show that a rejuvenated SED cannot reproduce the observed stacked spectra of (the bluer) quenched galaxies off the RS. We derive the upper limit on the fraction of massive galaxies on the RS at zeta approx 1.5 to be 2 and the zeta approx 1.5 RS. According to their estimated ages, the time required for quenched galaxies off the RS to join their counterparts on the z approx. 1.5 RS is of the order of approx. 1G/yr.

  9. Role of DNA polymerases eta, iota and zeta in UV resistance and UV-induced mutagenesis in a human cell line.

    Gueranger, Quentin; Stary, Anne; Aoufouchi, Saïd; Faili, Ahmad; Sarasin, Alain; Reynaud, Claude-Agnès; Weill, Jean-Claude


    Genes coding for DNA polymerases eta, iota and zeta, or for both Pol eta and Pol iota have been inactivated by homologous recombination in the Burkitt's lymphoma BL2 cell line, thus providing for the first time the total suppression of these enzymes in a human context. The UV sensitivities and UV-induced mutagenesis on an irradiated shuttle vector have been analyzed for these deficient cell lines. The double Pol eta/iota deficient cell line was more UV sensitive than the Pol eta-deficient cell line and mutation hotspots specific to the Pol eta-deficient context appeared to require the presence of Pol iota, thus strengthening the view that Pol iota is involved in UV damage translesion synthesis and UV-induced mutagenesis. A role for Pol zeta in a damage repair process at late replicative stages is reported, which may explain the drastic UV-sensitivity phenotype observed when this polymerase is absent. A specific mutation pattern was observed for the UV-irradiated shuttle vector transfected in Pol zeta-deficient cell lines, which, in contrast to mutagenesis at the HPRT locus previously reported, strikingly resembled mutations observed in UV-induced skin cancers in humans. Finally, a Pol eta PIP-box mutant (without its PCNA binding domain) could completely restore the UV resistance in a Pol eta deficient cell line, in the absence of UV-induced foci, suggesting, as observed for Pol iota in a Pol eta-deficient background, that TLS may occur without the accumulation of microscopically visible repair factories.

  10. Predicting the influence of liposomal lipid composition on liposome size, zeta potential and liposome-induced dendritic cell maturation using a design of experiments approach.

    Soema, Peter C; Willems, Geert-Jan; Jiskoot, Wim; Amorij, Jean-Pierre; Kersten, Gideon F


    In this study, the effect of liposomal lipid composition on the physicochemical characteristics and adjuvanticity of liposomes was investigated. Using a design of experiments (DoE) approach, peptide-containing liposomes containing various lipids (EPC, DOPE, DOTAP and DC-Chol) and peptide concentrations were formulated. Liposome size and zeta potential were determined for each formulation. Moreover, the adjuvanticity of the liposomes was assessed in an in vitro dendritic cell (DC) model, by quantifying the expression of DC maturation markers CD40, CD80, CD83 and CD86. The acquired data of these liposome characteristics were successfully fitted with regression models, and response contour plots were generated for each response factor. These models were applied to predict a lipid composition that resulted in a liposome with a target zeta potential. Subsequently, the expression of the DC maturation factors for this lipid composition was predicted and tested in vitro; the acquired maturation responses corresponded well with the predicted ones. These results show that a DoE approach can be used to screen various lipids and lipid compositions, and to predict their impact on liposome size, charge and adjuvanticity. Using such an approach may accelerate the formulation development of liposomal vaccine adjuvants.

  11. Prince of zeta by Petar Konjović: Opera in five/four acts on the 125th anniversary of the composer's birth

    Mosusova Nadežda


    Full Text Available Petar Konjović (Čurug, May 5, 1883 - Belgrade, October 1, 1970 stands out among Serbian composers as an author of instrumental and vocal compositions. Studies at the Prague Conservatory (1904-1906 acquainted Konjović with Czech music, Wagner's opus, and the Russian national-romantic school, which contributed to the evolution of his talent for both music and stage, enabling him to express his ideas more explicitly in operatic works. It was in the Prague that the second opera - Prince of Zeta - was conceived, with new musical vividness and dramatic appeal (first version composed 1906-1926, the second and final 1929-1939, followed by Koštana (1928, Peasants (1951 and Fatherland (1960. Konjović's mature operas are characterized by his masterful handling of form, both in close-ups and in detail, as well as his deeply individual assimilation of musical folklore into his work. The Prince of Zeta is not to be understood as a folk opera, but some main themes are directly derived from folk music, precisely from the Montenegrin folk songs quoted in the Mokranjac's Ninth Garland and treated in Konjović's post-romantic, almost expressionistic way, interwoven with some Italianate leitmotifs, so as to present the opera's two worlds, Montenegrin and Venetian. In the process of forming Konjović's operatic style, with vocal parts based mainly on the principle of declamation, the opera Prince of Zeta (first performed in Belgrade, 1929, conducted by Lovro von Matačić proved to be a work of great impact. Hardly anyone grasped then the wide sweep of inspiration which allowed the composer to set and to solve several important problems connected with music drama, essential also in his subsequent stage works. First of all, Konjović had to handle in his own way the verbal drama the prototype of his opera, Maxim Crnojević by the Serbian poet Laza Kostić (1841-1910. Permission came from the playwright in the first decade of the 1900, Prince of Zeta being partly

  12. Cyclic Variability of the Circumstellar Disc of the Be Star $\\zeta$ Tau. II. Testing the 2D Global Disc Oscillation Model

    Carciofi, A C; Bouquin, J-B le; Štefl, S; Rivinius, Th; Baade, D; Björkman, J E; Hummel, C A


    Aims. In this paper we model, in a self-consistent way, polarimetric, photometric, spectrophotometric and interferometric observations of the classical Be star $\\zeta$ Tauri. Our primary goal is to conduct a critical quantitative test of the global oscillation scenario. Methods. We have carried out detailed three-dimensional, NLTE radiative transfer calculations using the radiative transfer code HDUST. For the input for the code we have used the most up-to-date research on Be stars to include a physically realistic description for the central star and the circumstellar disc. We adopt a rotationally deformed, gravity darkened central star, surrounded by a disc whose unperturbed state is given by a steady-state viscous decretion disc model. We further assume that disc is in vertical hydrostatic equilibrium. Results. By adopting a viscous decretion disc model for $\\zeta$ Tauri and a rigorous solution of the radiative transfer, we have obtained a very good fit of the time-average properties of the disc. This prov...

  13. Mixtures of latex particles and the surfactant of opposite charge used as interface stabilizers--influence of particle contact angle, zeta potential, flocculation and shear energy.

    Deleurence, Rémi; Parneix, Caroline; Monteux, Cécile


    We investigate the stabilization of air-water interfaces by mixtures of negatively charged latex particles (sulfate polystyrene) and cationic surfactants (alkyl trimethylammonium bromides). First we report results concerning the binding of surfactant molecules to the latex particles. As the surfactant concentration increases, the charge of the particles reverses, from negative to positive, because CnTAB first binds electrostatically to the latex particles and then through hydrophobic interaction with the monolayer already adsorbed on the particles as well as directly with the hydrophobic surface of the latex. Over a large range of surfactant concentrations around the charge inversion, a strong flocculation is observed and 100 μm large aggregates form in the suspension. Unlike previous studies published on mixtures of inorganic particles with oppositely charged surfactants, we show that we can vary the sign of the zeta potential of the particles without changing the contact angle of the particles over a large range of surfactant concentrations. Indeed, the latex particles that we study are more hydrophobic than inorganic particles, hence adding moderate concentrations of the surfactant results in a weak variation of the contact angle while the charge of the particles can be reversed. This enables decoupling of the effect of zeta potential and contact angle on the interfacial properties of the mixtures. Our study shows that the contact angle and the charge of the particles are not sufficient parameters to control the foam properties, and the key-parameters are the flocculation state and the shear energy applied to produce the foam. Indeed, flocculated samples, whatever the sign of the zeta potential, enable production of a stable armour at the interface. The large aggregates do not adsorb spontaneously at the interface because of their large size, however when a large shear energy is used to produce the foam very stable foam is obtained, where particles are trapped

  14. 2-(6-Phenyl-1H-indazol-3-yl)-1H-benzo[d]imidazoles: Design and synthesis of a potent and isoform selective PKC-[zeta] inhibitor

    Trujillo, John I.; Kiefer, James R.; Huang, Wei; Thorarensen, Atli; Xing, Li; Caspers, Nicole L.; Day, Jacqueline E.; Mathis, Karl J.; Kretzmer, Kuniko K.; Reitz, Beverley A.; Weinberg, Robin A.; Stegeman, Roderick A.; Wrightstone, Ann; Christine, Lori; Compton, Robert; Li, Xiong; (Pfizer)


    The inhibition of PKC-{zeta} has been proposed to be a potential drug target for immune and inflammatory diseases. A series of 2-(6-phenyl-1H indazol-3-yl)-1H-benzo[d]imidazoles with initial high crossover to CDK-2 has been optimized to afford potent and selective inhibitors of protein kinase c-zeta (PKC-{zeta}). The determination of the crystal structures of key inhibitor:CDK-2 complexes informed the design and analysis of the series. The most selective and potent analog was identified by variation of the aryl substituent at the 6-position of the indazole template to give a 4-NH{sub 2} derivative. The analog displays good selectivity over other PKC isoforms ({alpha}, {beta}II, {gamma}, {delta}, {epsilon}, {mu}, {theta}, {eta} and {ell}/{lambda}) and CDK-2, however it displays marginal selectivity against a panel of other kinases (37 profiled).

  15. Genomic Insights Into the First Cultured Member of the Zeta-Proteobacteria, the Fe-Oxidizing Mariprofundus Ferrooxydans PV-1

    Singer, E.; Emerson, D.; Webb, E.; Nelson, W.; Heidelberg, J.; Kuenen, G.; Edwards, K. J.


    We present the genome of Mariprofundus ferrooxydans PV-1, the first cultured representative in the novel candidatus class Zetaproteobacteria to date. Isolated from iron mats in 1,100m depth at the Loihi Seamount, Hawai’i, M. ferrooxydans is known as a microaerophilic, obligate iron oxidizer. Its most striking feature is the formation of a highly structured and localized stalk comprised primarily of iron oxides and an organic matrix. During its cell cycle, M. ferrooxydans alternates between forming attached stalks and free-living cells. The genome appears deeply-rooted within the Proteobacteria, as conserved structural and functional genes, for example encoding key enzymes, share less than 40% similarity with homologs found in Alpha-, Beta-, Delta-, and Gammaproteobacteria. Genome analysis revealed a complete TCA cycle, the ability to fix CO2, carbon-storage proteins and a sugar phosphotransferase system (PTS), which may facilitate the transport of carbohydrates across the cell membrane and possibly aid in stalk formation. Two-component signal transduction system genes, including histidine kinases, GGDEF domain genes, and response regulators containing CheY-like receivers, are abundant and widely distributed across the genome. Most of these are located in close proximity to genes involved in cell division, phosphate uptake and transport, exopolymer and heavy metal secretion, flagellar biosynthesis and pilus assembly suggesting that these functions are highly regulated. Several cytochromes encode for an electron transport chain potentially involved in Fe(II)-oxidation. Antioxidant genes, such as super oxide dismutases and peroxidases, as well as aerotaxis sensory genes encode for the potential to regulate functions in response to oxygen gradients, such as would be required to maintain cellular redox balance in the specialized habitat M. ferrooxydans resides. The first exploration of the genetic potential of M. ferrooxydans PV-1 will serve as a foundation for

  16. Redirecting Therapeutic T Cells against Myelin-Specific T Lymphocytes Using a Humanized Myelin Basic Protein-HLA-DR2-{zeta} Chimeric Receptor

    Moisini, Ioana; Nguyen, Phuong; Fugger, Lars


    Therapies that Ag-specifically target pathologic T lymphocytes responsible for multiple sclerosis (MS) and other autoimmune diseases would be expected to have improved therapeutic indices compared with Ag-nonspecific therapies. We have developed a cellular immunotherapy that uses chimeric receptors...... to selectively redirect therapeutic T cells against myelin basic protein (MBP)-specific T lymphocytes implicated in MS. We generated two heterodimeric receptors that genetically link the human MBP(84-102) epitope to HLA-DR2 and either incorporate or lack a TCRzeta signaling domain. The Ag-MHC domain serves...... as a bait, binding the TCR of MBP-specific target cells. The zeta signaling region stimulates the therapeutic cell after cognate T cell engagement. Both receptors were well expressed on primary T cells or T hybridomas using a tricistronic (alpha, beta, green fluorescent protein) retroviral expression system...

  17. Sobre los ceros de polinomios de Dirichlet, en general, y los de las sumas parciales de la función zeta de Riemann, en particular

    Dubon, Eric


    En el primer capítulo se introduce la función $H_{n}(z)=1+2^{iz}+3^{iz}+...+n^{iz}$ como aproximación de la función zeta de Riemann y se pondrá de relieve una de sus principales propiedades, que es la de ser una función entera de tipo exponencial de clase C. Se presenta, utilizando la noción de distribución de Levinson, una demostración de la densidad de ceros de este tipo de funciones distinta a la obtenida por los autores de [41]. Se dará también, con la condición de existencia de ceros sob...

  18. Signatures of quantum-like chaos in spacing intervals of non-trivial Riemann zeta zeros and in turbulent fluid flows

    Selvam, A M


    The spacing intervals of adjacent Riemann zeta zeros(non-trivial) exhibit fractal(irregular) fluctuations generic to dynamical systems in nature such as fluid flows, heart beat patterns, stock market price index, etc., and are associated with unpredictability or chaos. The power spectra of such fractal space-time fluctuations exhibit universal inverse power law form and signify long-range correlations, identified as self-organized criticality . A cell dynamical system model developed by the author for turbulent fluid flows provides a unique quantification for the observed power spectra in terms of the statistical normal distribution, such that the variance represents the statistical probability densities. Such a result that the additive amplitudes of eddies when squared, represent the statistical probabilities is an observed feature of the subatomic dynamics of quantum systems such as an electron or photon. Self-organized criticality is therefore a signature of quantum-like chaos in dynamical systems. The mod...

  19. PD-1 inhibits T-cell receptor induced phosphorylation of the ZAP70/CD3zeta signalosome and downstream signaling to PKCtheta.

    Sheppard, Kelly-Ann; Fitz, Lori J; Lee, Julie M; Benander, Christina; George, Judith A; Wooters, Joe; Qiu, Yongchang; Jussif, Jason M; Carter, Laura L; Wood, Clive R; Chaudhary, Divya


    Engagement of the immunoinhibitory receptor, programmed death-1 (PD-1) attenuates T-cell receptor (TCR)-mediated activation of IL-2 production and T-cell proliferation. Here, we demonstrate that PD-1 modulation of T-cell function involves inhibition of TCR-mediated phosphorylation of ZAP70 and association with CD3zeta. In addition, PD-1 signaling attenuates PKCtheta activation loop phosphorylation in a cognate TCR signal. PKCtheta has been shown to be required for T-cell IL-2 production. A phosphorylated PD-1 peptide, corresponding to the C-terminal immunoreceptor tyrosine-switch motif (ITSM), acts as a docking site in vitro for both SHP-2 and SHP-1, while the phosphorylated peptide containing the N-terminal PD-1 immunoreceptor tyrosine based inhibitory motif (ITIM) associates only with SHP-2.

  20. Structural mutations of C-domains in members of the Ig superfamily. Consequences for the interactions between the T cell antigen receptor and the zeta 2 homodimer

    Geisler, C; Rubin, B; Caspar-Bauguil, S;


    not fully understood. We locate critical amino acid residues for TCR assembly in the Ti-alpha and -beta extracellular C-domains. A point mutation (phenylalanine195----valine) in a highly conserved residue in the Ti-alpha chain of the Jurkat variant J79 was identified by DNA sequencing. This mutation did......-alpha-deficient Jurkat variant. Computer model analysis showed that the Ti-alpha phenylalanine195 directly contributed to the beta-sheet facing away from the Ti-beta chain, indicating that it could be directly involved in the interactions between one or more of the CD3 chains or the zeta 2 dimer. Site......-directed mutagenesis of the corresponding residue in the Ti-beta chain demonstrated that a phenylalanine216----valine substitution had similar effects on TCR assembly as the Ti-alpha mutation, whereas a phenylalanine216----histidine substitution allowed TCR assembly and expression. Whether the consequences for TCR...

  1. Organelles and chromatin fragmentation of human umbilical vein endothelial cell influence by the effects of zeta potential and size of silver nanoparticles in different manners.

    Tavakol, Shima; Hoveizi, Elham; Kharrazi, Sharmin; Tavakol, Behnaz; Karimi, Shabnam; Rezayat Sorkhabadi, Seyed Mahdi


    Recently, it has been disclosed that silver nanoparticles (AgNPs) have the potential to inhibit infection and cancerous cells and eventually penetrate through injected site into the capillary due to their small size. This study focuses on the effect of size and zeta potential of bare and citrate-coated AgNPs on human umbilical vein endothelial cells (HUVECs) as main capillary cells. AgNPs with high and low concentrations and no citrate coating were synthesized by using simple wet chemical method and named as AgNP/HC, AgNP/LC, and AgNP, respectively. Citrate coated particles showed larger zeta potential of -22 mV and AgNp/HC showed the smallest size of 13.2 nm. UV-Visible spectroscopy and dynamic light scattering (DLS) were performed to evaluate particle size and hydrodynamic diameter of NPs in water and cell culture media. Results indicated that higher concentrations of citrate decreased hydrodynamic diameter and NP agglomeration. reactive oxygen species (ROS) production of all AgNPs was similar at 28 ppm although it was significantly higher than control group. Their effects on cell membrane and chromosomal structure were studied using LDH measurement and 4',6-diamidino-2-phenylindole (DAPI) staining, as well. Results demonstrated that AgNP/LC was less toxic to cells owing to higher value of IC50, minimum inhibitory concentration (MIC), and less release of LDH. Cancerous (Human Caucasian neuroblastoma) and immortal cells (Mouse embryonic fibroblast cell line) were about twice more sensitive than HUVECs to toxic effects of AgNPs. DAPI staining results showed that AgNP and AgNP/HC induced highest and lowest breaking of chromosome. Overall results suggest that viability of HUVECs will be higher than 90% when viability of cancerous cells is 50% in AgNPs chemotherapy.

  2. Methamphetamine-induced short-term increase and long-term decrease in spatial working memory affects Protein Kinase M zeta (PKMζ, dopamine, and glutamate receptors

    Stephen H Braren


    Full Text Available Methamphetamine (MA is a toxic, addictive drug shown to modulate learning and memory, yet the neural mechanisms are not fully understood. We investigated the effects of 2 weekly injections of MA (30 mg/kg on working memory using the radial 8-arm maze (RAM across 5 weeks in adolescent-age mice. MA-treated mice show a significant improvement in working memory performance 1 week following the first MA injection compared to saline-injected controls. Following 5 weeks of MA abstinence mice were re-trained on a reference and working memory version of the RAM to assess cognitive flexibility. MA-treated mice show significantly more working memory errors without effects on reference memory performance. The hippocampus and dorsal striatum were assessed for expression of glutamate receptors subunits, GluA2 and GluN2B; dopamine markers, dopamine 1 receptor (D1, dopamine transporter (DAT and tyrosine hydroxylase (TH; and memory markers, protein kinase M zeta (PKMζ and protein kinase C zeta (PKCζ. Within the hippocampus, PKMζ and GluA2 are both significantly reduced after MA supporting the poor memory performance. Additionally, a significant increase in GluN2B and decrease in D1 identifies dysregulated synaptic function. In the striatum, MA treatment increased cytosolic DAT and TH levels associated with dopamine hyperfunction. MA treatment significantly reduced GluN2B while increasing both PKMζ and PKCζ within the striatum. We discuss the potential role of PKMζ/PKCζ in modulating dopamine and glutamate receptors after MA treatment. These results identify potential underlying mechanisms for working memory deficits induced by MA.

  3. Elucidation of the mechanisms of action of Bacteriophage K/nano-emulsion formulations against S. aureus via measurement of particle size and zeta potential.

    Esteban, Patricia Perez; Jenkins, A Toby A; Arnot, Tom C


    In earlier work we have demonstrated the effect that nano-emulsions have on bacterial growth, and most importantly the enhanced bacteriophage infectivity against Staphylococcus aureus in planktonic culture when phage are carried in nano-emulsions. However, the mechanisms of enhancement of the bacteriophage killing effect are not specifically understood. This work focuses on the investigation of the possible interactions between emulsion droplets and bacterial cells, between emulsion droplets and bacteriophages, and finally interactions between all three components: nano-emulsion droplets, bacteria, and bacteriophages. The first approach consists of simple calculations to determine the spatial distribution of the components, based on measurements of particle size. It was found that nano-emulsion droplets are much more numerous than bacteria or bacteriophage, and due to their size and surface area they must be covering the surface of both cells and bacteriophage particles. Stabilisation of bacteriophages due to electrostatic forces and interaction with nano-emulsion droplets is suspected, since bacteriophages may be protected against inactivation due to 'charge shielding'. Zeta potential was measured for the individual components in the system, and for all of them combined. It was concluded that the presence of nano-emulsions could be reducing electrostatic repulsion between bacterial cells and bacteriophage, both of which are very negatively 'charged'. Moreover, nano-emulsions lead to more favourable interaction between bacteriophages and bacteria, enhancing the anti-microbial or killing effect. These findings are relevant since the physicochemical properties of nano-emulsions (i.e. particle size distribution and zeta potential) are key in determining the efficacy of the formulation against infection in the context of responsive burn wound dressings-which is the main target for this work.

  4. Use of Spectroscopic, Zeta Potential and Molecular Dynamic Techniques to Study the Interaction between Human Holo-Transferrin and Two Antagonist Drugs: Comparison of Binary and Ternary Systems

    Mohammad Reza Saberi


    Full Text Available For the first time, the binding of ropinirole hydrochloride (ROP and aspirin (ASA to human holo-transferrin (hTf has been investigated by spectroscopic approaches (fluorescence quenching, synchronous fluorescence, time-resolved fluorescence, three-dimensional fluorescence, UV-vis absorption, circular dichroism, resonance light scattering, as well as zeta potential and molecular modeling techniques, under simulated physiological conditions. Fluorescence analysis was used to estimate the effect of the ROP and ASA drugs on the fluorescence of hTf as well as to define the binding and quenching properties of binary and ternary complexes. The synchronized fluorescence and three-dimensional fluorescence spectra demonstrated some micro-environmental and conformational changes around the Trp and Tyr residues with a faint red shift. Thermodynamic analysis displayed the van der Waals forces and hydrogen bonds interactions are the major acting forces in stabilizing the complexes. Steady-state and time-resolved fluorescence data revealed that the fluorescence quenching of complexes are static mechanism. The effect of the drugs aggregating on the hTf resulted in an enhancement of the resonance light scattering (RLS intensity. The average binding distance between were computed according to the forster non-radiation energy transfer theory. The circular dichroism (CD spectral examinations indicated that the binding of the drugs induced a conformational change of hTf. Measurements of the zeta potential indicated that the combination of electrostatic and hydrophobic interactions between ROP, ASA and hTf formed micelle-like clusters. The molecular modeling confirmed the experimental results. This study is expected to provide important insight into the interaction of hTf with ROP and ASA to use in various toxicological and therapeutic processes.

  5. Methamphetamine-induced short-term increase and long-term decrease in spatial working memory affects protein Kinase M zeta (PKMζ), dopamine, and glutamate receptors.

    Braren, Stephen H; Drapala, Damian; Tulloch, Ingrid K; Serrano, Peter A


    Methamphetamine (MA) is a toxic, addictive drug shown to modulate learning and memory, yet the neural mechanisms are not fully understood. We investigated the effects of 2 weekly injections of MA (30 mg/kg) on working memory using the radial 8-arm maze (RAM) across 5 weeks in adolescent-age mice. MA-treated mice show a significant improvement in working memory performance 1 week following the first MA injection compared to saline-injected controls. Following 5 weeks of MA abstinence mice were re-trained on a reference and working memory version of the RAM to assess cognitive flexibility. MA-treated mice show significantly more working memory errors without effects on reference memory performance. The hippocampus and dorsal striatum were assessed for expression of glutamate receptors subunits, GluA2 and GluN2B; dopamine markers, dopamine 1 receptor (D1), dopamine transporter (DAT) and tyrosine hydroxylase (TH); and memory markers, protein kinase M zeta (PKMζ) and protein kinase C zeta (PKCζ). Within the hippocampus, PKMζ and GluA2 are both significantly reduced after MA supporting the poor memory performance. Additionally, a significant increase in GluN2B and decrease in D1 identifies dysregulated synaptic function. In the striatum, MA treatment increased cytosolic DAT and TH levels associated with dopamine hyperfunction. MA treatment significantly reduced GluN2B while increasing both PKMζ and PKCζ within the striatum. We discuss the potential role of PKMζ/PKCζ in modulating dopamine and glutamate receptors after MA treatment. These results identify potential underlying mechanisms for working memory deficits induced by MA.

  6. Zeta Potential Study on the Bio-oxidation of Copper Sulfide Minerals%硫化铜矿细菌氧化的Zeta电位研究

    傅开彬; 林海


    为了研究矿物与细菌相互作用后矿物的Zeta电位与细菌对矿物的氧化能力之间的关系,利用Zeta电位仪检测与细菌作用前后硫化矿的Zeta电位,根据Zeta电位和IEP(Iso Electric Point)的变化,判断细菌对硫化矿的氧化作用及其程度。结果表明,硫化铜矿与细菌作用后,矿物的Zeta电位降低,辉铜矿、斑铜矿和铜蓝的IEP向铜的氢氧化物方向移动,而黄铜矿向细菌的IEP方向靠近,分析原因主要是由于各矿物被细菌氧化的程度不同。对与细菌相互作用后的铜蓝进行XPS测试结果证实其表面被氧化。研究证实了细菌对硫化矿的直接氧化作用,同时也得出不同硫化铜的细菌氧化顺序:辉铜矿〉斑铜矿〉铜蓝〉黄铜矿。%The direct bacterial oxidation of sulphide minerals was evaluated by Zeta potential and the iso- electric point (IEP). The results show that copper sulphide minerals have been directly oxidized by the bacteria, after conditioning with Acidithiobacillus ferrooxidans, the Zeta potential of copper sulphide de- creased and became negative, the IEP of chalcoeite, bornite and covellite moved towards the IEP of cop- per hydroxide, however, the IEP of chalcopyrite was very close to that of bacteria. The XPS of covellite after conditioning with bacteria indicates the surface was oxidized. The results support the direct mecha- nism of sulphide minerals bio-oxidation from a new viewpoint. The following order for the bio-oxidation of these minerals is obtained ~ chalcocite 〉 bornite 〉 covellite 〉 chalcoovrite.

  7. Dietary fish oil blocks carcinogen-induced down-regulation of colonic protein kinase C isozymes.

    Jiang, Y H; Lupton, J R; Chapkin, R S


    In order to elucidate the influence of dietary constituents on colonic intracellular signal transduction, the effect of different fats on rat colonic epithelial protein kinase C (PKC) alpha (classical), delta (novel) and lambda-zeta (atypical) expression was determined in carcinogen-treated animals. Sprague-Dawley rats were provided with one of two fats (corn oil and fish oil); plus or minus the carcinogen azoxymethane (AOM) and killed at two time points (15 and 37 weeks) in a 2x2x2 factorial design. At 5 and 6 weeks of age, animals were injected s.c. with either AOM at a dose of 15 mg/kg body weight or saline once a week for 2 weeks and continued on the same diet until termination of the study. At 15 and 37 weeks after the second injection, 10 rats from each treatment group were killed. Colonic PKC alpha, delta and lambda-zeta steady-state protein and mRNA levels were determined using immunoblotting and relative quantitative polymerase chain reaction, respectively. Colonic mucosa from rats injected with AOM had significantly suppressed membrane and cytosolic PKC alpha and cytosolic lambda-zeta protein levels (P fish oil diets had significantly higher (P protein levels relative to animals fed corn oil diets. However, the effect of diet and AOM on the steady-state expression of PKC alpha, delta and zeta mRNA was not consistent with changes in the respective isozyme protein levels, suggesting regulation at the post-transcriptional level. These data demonstrate that dietary fish oil blocks the carcinogen-induced decrease in the steady-state levels of colonic mucosal PKC delta and lambda-zeta, which may in part explain why this fat source protects against colon cancer development.

  8. SUMO: regulating the regulator

    Bossis Guillaume


    Full Text Available Abstract Post-translational modifiers of the SUMO (Small Ubiquitin-related Modifier family have emerged as key regulators of protein function and fate. While the past few years have seen an enormous increase in knowledge on SUMO enzymes, substrates, and consequences of modification, regulation of SUMO conjugation is far from being understood. This brief review will provide an overview on recent advances concerning (i the interplay between sumoylation and other post-translational modifications at the level of individual targets and (ii global regulation of SUMO conjugation and deconjugation.

  9. Stability Investigation of Ligand-Exchanged CdSe/ZnS-Y (Y = 3-Mercaptopropionic Acid or Mercaptosuccinic Acid through Zeta Potential Measurements

    Ngoc Thuy Vo


    Full Text Available Quantum dots have been considered to be promising candidates for bioapplications because of their high sensitivity, rapid response, and reliability. The synthesis of high-quality quantum dots that can be dissolved in water and other biological media is a crucial step toward their further application in biology. Starting with a one-pot reaction and the successive ionic layer adsorption and reaction (SILAR method, we produced the CdSe/ZnS core/shell structure. Through a ligand-exchange mechanism, we coated the as-made CdSe/ZnS structure with 3-mercaptopropionic acid (MPA or mercaptosuccinic acid (MSA. Various techniques, including photoluminescence (PL, ultraviolet-visible (UV-Vis spectroscopy, transmission electron microscopy (TEM, X-ray diffraction (XRD, and Fourier transform infrared (FTIR spectroscopy, were utilized to characterize the ligand-coated CdSe/ZnS structure. The results show enhanced luminescence intensity, CdSe surface passivation by ZnS, and successful coating with MPA and MSA. The stability of quantum dots in solutions with different pH values was investigated by performing zeta potential measurements. The results revealed that the quantum dots shifted from displaying hydrophobic to hydrophilic behavior and could be connected with bioagents.




    Full Text Available La calidad del agua cruda del Río Otún que abastece la planta de tratamiento de agua potable de Aguas y Aguas de Pereira puede presentar fluctuaciones en cortos periodos de tiempo. Por esta razón se requiere un instrumento que permita tomar decisiones rápidas para modificar la dosis de coagulante a utilizar en el tratamiento con base en un criterio cuantificable y preciso como el potencial zeta (ζ. En este estudio se determinó que para un intervalo de ζ desde -2.28 mV a +1.2 mV del agua coagulada se obtienen valores bajos de turbiedad y color del agua sedimentada. Es posible alcanzar un óptimo desempeño del proceso de floculación y sedimentación efectuando mediciones de ζ en el agua coagulada como indicador de desestabilización de las partículas. ζ presenta una correlación positiva con la dosis de coagulante aplicada, modificando la dosis de coagulante es posible alcanzar valores de ζ cercanos a cero.

  11. Genetic polymorphism of 3' untranslated region of zeta-chain associated protein kinase 70 kDa in southern Taiwanese patients with rheumatoid arthritis.

    Chen, Shih-Yao; Liu, Ming-Fei; Wang, Chrong-Reen


    T cell activation participates in the pathogenesis of rheumatoid arthritis (RA), and the signaling molecule zeta-chain-associated protein kinase 70 kDa (ZAP-70) plays a crucial role in this process. Different mutations in the coding sequence of ZAP-70 are involved in a variety of immunological phenotypes, and recent evidence indicates that genetic variations within the 3' untranslated regions (UTR) of microRNA binding sites may affect the hybridization with target mRNAs, leading to phenotype changes with disease status. In this study, we evaluated the possible effect of ZAP-70 polymorphism as a genetic risk factor in RA by examining the single-nucleotide polymorphism in 100 patients and 100 ethnicity- and sex-matched healthy individuals from southern Taiwan. In both groups, the genotype distribution of rs2278699 in the 3' UTR was in the Hardy-Weinberg equilibrium. In RA, there were higher frequencies of the G allele (15.5 versus 8.0 %, odds ratio 2.1, P = 0.020) and significant differences in the trend of various genotypes (P = 0.024). The results suggest that genetic polymorphism in the 3' UTR of ZAP-70 is associated with RA susceptibility in southern Taiwanese.

  12. On the Inference of the Cosmic Ray Ionization Rate $\\zeta$ from the HCO$^+$-to-DCO$^+$ Abundance Ratio: The Effect of Nuclear Spin

    Shingledecker, Christopher N; Gal, Romane Le; Oberg, Karin I; Hincelin, Ugo; Herbst, Eric


    The chemistry of dense interstellar regions was analyzed using a time-dependent gas-grain astrochemical simulation and a new chemical network that incorporates deuterated chemistry taking into account nuclear spin-states for the hydrogen chemistry and its deuterated isotopologues. With this new network, the utility of the [HCO$^+$]/[DCO$^+$] abundance ratio as a probe of the cosmic ray ionization rate has been reexamined, with special attention paid to the effect of the initial value of the molecular hydrogen ortho-to-para ratio (OPR). After discussing the use of the probe for cold cores, we then compare our results with previous theoretical and observational results for a molecular cloud close to the supernova remnant W51C, which is thought to have an enhanced cosmic ray ionization rate $\\zeta$ caused by the nearby $\\gamma$-ray source. In addition, we attempt to use our approach to estimate the cosmic ray ionization rate for L1174, a dense core with an embedded star. Beyond the previously known sensitivity o...

  13. Effect of chemical composition on hydrophobicity and zeta potential of plasma sprayed HA/CaO-P2O5 glass coatings.

    Ferraz, M P; Monteiro, F J; Serro, A P; Saramago, B; Gibson, I R; Santos, J D


    Multilayered plasma sprayed coatings on the surface of Ti-6Al-4V alloys have been prepared, which were composed of an underlayer of HA and a surface layer of a CaO-P2O5 glass-HA composite, with 2 or 4wt% of glass. Contact angle and surface tension variation with time, for both water and a protein solution, were determined by the sessile and pendent drop methods respectively using the ADSA-P software. Wettability studies showed that hydrophobicity of the coatings increase with the glass addition. The work of adhesion of albumin was also altered in a controlled manner by the addition of the CaO-P2O5 glass, being lower on the composite coatings than on HA. Zeta potential (ZP) results showed that composite coatings presented a higher net negative charge than HA coatings and that ZP values were also influenced by the content of the glass. This study demonstrated that the surface properties of those coatings may be modified by the addition of CaO-P2O5 glass.

  14. The preparation, surface structure, zeta potential, surface charge density and photocatalytic activity of TiO{sub 2} nanostructures of different shapes

    Grover, Inderpreet Singh; Singh, Satnam; Pal, Bonamali, E-mail:


    Titania based nanocatalysts such as sodium titanates of different morphology having superior surface properties are getting wide importance in photocatalysis research. Despite having sodium (Na) contents and its high temperature synthesis (that generally deteriorate the photoreactivity), these Na-titanates often exhibit better photoactivity than P25-TiO{sub 2} catalyst. Hence, this work demonstrated the influence of crystal structure, BET surface area, surface charge, zeta potential (ζ) and metal loading on the photocatalytic activity of as-prepared sodium titanate nanotube (TNT) and titania nanorod (TNR). Straw like hollow orthorhombic-TNT (Na{sub 2}Ti{sub 2}O{sub 5}·H{sub 2}O) particles (W = 9–12 nm and L = 82–115 nm) and rice like pure anatase-TNR particles (W = 8–13 nm and L = 81–134 nm) are obtained by the hydrothermal treatment of P25-TiO{sub 2} with NaOH, which in fact, altered the net surface charge of TNT and TNR particles. The observed ζ = −2.82 (P25-TiO{sub 2}), −13.5 (TNT) and −22.5 mV (TNR) are significantly altered by the Ag and Cu deposition. It has been found here that TNT displayed best photocatalytic activity for the imidacloprid insecticide (C{sub 9}H{sub 10}ClN{sub 5}O{sub 2}) degradation to CO{sub 2} formation under UV irradiation because of its largest surface area 176 m{sup 2} g{sup −1} among the catalysts studied.

  15. Structural analysis of intermolecular interactions in the kinesin adaptor complex fasciculation and elongation protein zeta 1/ short coiled-coil protein (FEZ1/SCOCO.

    Marcos Rodrigo Alborghetti

    Full Text Available Cytoskeleton and protein trafficking processes, including vesicle transport to synapses, are key processes in neuronal differentiation and axon outgrowth. The human protein FEZ1 (fasciculation and elongation protein zeta 1 / UNC-76, in C. elegans, SCOCO (short coiled-coil protein / UNC-69 and kinesins (e.g. kinesin heavy chain / UNC116 are involved in these processes. Exploiting the feature of FEZ1 protein as a bivalent adapter of transport mediated by kinesins and FEZ1 protein interaction with SCOCO (proteins involved in the same path of axonal growth, we investigated the structural aspects of intermolecular interactions involved in this complex formation by NMR (Nuclear Magnetic Resonance, cross-linking coupled with mass spectrometry (MS, SAXS (Small Angle X-ray Scattering and molecular modelling. The topology of homodimerization was accessed through NMR (Nuclear Magnetic Resonance studies of the region involved in this process, corresponding to FEZ1 (92-194. Through studies involving the protein in its monomeric configuration (reduced and dimeric state, we propose that homodimerization occurs with FEZ1 chains oriented in an anti-parallel topology. We demonstrate that the interaction interface of FEZ1 and SCOCO defined by MS and computational modelling is in accordance with that previously demonstrated for UNC-76 and UNC-69. SAXS and literature data support a heterotetrameric complex model. These data provide details about the interaction interfaces probably involved in the transport machinery assembly and open perspectives to understand and interfere in this assembly and its involvement in neuronal differentiation and axon outgrowth.

  16. Structure-based lead discovery for protein kinase C zeta inhibitor design by exploiting kinase-inhibitor complex crystal structure data and potential therapeutics for preterm labour.

    Shao, Qing-Chun; Zhang, Cui-Juan; Li, Jie


    The protein kinase C (PKC) is a family of serine/threonine kinases with a broad range of cellular targets. Members of the PKC family participate at the diverse biological events involved in cellular proliferation, differentiation and survival. The PKC isoform zeta (PKCζ) is an atypical member that has recently been found to play an essential role in promoting human uterine contractility and thus been raised as a new target for treating preterm labour and other tocolytic diseases. In this study, an integrative protocol was described to graft hundreds of inhibitor ligands from their complex crystal structures with cognate kinases into the active pocket of PKCζ and, based on the modeled structures, to evaluate the binding strength of these inhibitors to the non-cognate PKCζ receptor by using a consensus scoring strategy. A total of 32 inhibitors with top score were compiled, and eight out of them were tested for inhibitory potency against PKCζ. Consequently, five compounds, i.e. CDK6 inhibitor fisetin, PIM1 inhibitor myricetin, CDK9 inhibitor flavopiridol and PknB inhibitor mitoxantrone as well as the promiscuous kinase inhibitor staurosporine showed high or moderate inhibitory activity on PKCζ, with IC50 values of 58 ± 9, 1.7 ± 0.4, 108 ± 17, 280 ± 47 and 0.019 ± 0.004 μM, respectively, while other three compounds, including two marketed drugs dasatinib and sunitinib as well as the Rho inhibitor fasudil, have not been detected to possess observable activity. Next, based on the modeled structure data we modified three flavonoid kinase inhibitors, i.e. fisetin, myricetin and flavopiridol, to generate a number of more potential molecular entities, two of which were found to have a moderately improved activity as compared to their parent compounds.

  17. Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report.

    Chithiwala, Zahabiya H; Lee, Hoi Chang; Hill, David L; Jellerette-Nolan, Teru; Fissore, Rafael; Grow, Daniel; Dumesic, Daniel A


    The purpose of this study is to describe impaired oocyte fertilization from phospholipase C-zeta (PLC-ζ) deficiency in normal-appearing sperm that was successfully treated using calcium (Ca(2+)) ionophore with intracytoplasmic sperm injection (ICSI) of oocytes matured in vitro. An infertile couple undergoing in vitro fertilization (IVF) experienced failed oocyte fertilization following ICSI with normal-appearing sperm. A semen sample collected from the patient was used to assess the expression of sperm PLC- ζ protein by Western blot analysis and immunofluorescence and PLC-ζ bioactivity by an in vitro model of Ca(2+) release. A second IVF cycle was performed using Ca(2+) ionophore with ICSI to enhance Ca(2+)-induced oocyte activation of oocytes matured in vitro. Sperm PLC-ζ protein deficiency was demonstrated by Western blot analysis and immunofluorescence and confirmed by reduced PLC-ζ bioactivity using an in vitro model of Ca(2+) release. Nevertheless, with this sperm and supplementation of Ca(2+) ionophore following ICSI, fertilization of four of six oocytes matured in vitro was obtained. In addition, four embryos underwent cleavage and two of them reached the blastocyst stage. Transfer of these blastocysts into the uterus led to a single pregnancy and live birth. Deficiency of PLC-ζ in normal-appearing human sperm is associated with impaired Ca(2+)-dependent oocyte activation during ICSI. Under this condition, use of Ca(2+) ionophore following ICSI of oocytes matured in vitro improves embryo developmental competence, possibly through the activation of Ca(2+)-dependent mechanisms governing fertilization and preimplantation embryogenesis.

  18. zeta-, epsilon-, and gamma-Globin mRNA in blood samples and CD71(+) cell fractions from fetuses and from pregnant and nonpregnant women, with special attention to identification of fetal erythroblasts

    Høgh, A M; Hviid, T V; Christensen, B


    nonpregnant and 10 of 14 pregnant women (at 9-13 weeks of gestation) were positive for gamma-globin mRNA (Fisher's exact test, P = 0.13), and those from 3 of 20 nonpregnant and 5 of 14 pregnant women were positive for zeta-globin mRNA (Fisher's exact test, P = 0.23). No epsilon-globin mRNA was detected in CD...

  19. A zeta potential value determines the aggregate’s size of penta-substituted [60]fullerene derivatives in aqueous suspension whereas positive charge is required for toxicity against bacterial cells

    Deryabin, Dmitry G.; Ludmila V. Efremova; Vasilchenko, Alexey S.; Saidakova, Evgeniya V; Sizova, Elena A.; Pavel A. Troshin; Zhilenkov, Alexander V; Khakina, Ekaterina E


    Background The cause–effect relationships between physicochemical properties of amphiphilic [60]fullerene derivatives and their toxicity against bacterial cells have not yet been clarified. In this study, we report how the differences in the chemical structure of organic addends in 10 originally synthesized penta-substituted [60]fullerene derivatives modulate their zeta potential and aggregate’s size in salt-free and salt-added aqueous suspensions as well as how these physicochemical characte...

  20. Lysine N[superscript zeta]-Decarboxylation Switch and Activation of the [beta]-Lactam Sensor Domain of BlaR1 Protein of Methicillin-resistant Staphylococcus aureus

    Borbulevych, Oleg; Kumarasiri, Malika; Wilson, Brian; Llarrull1, Leticia I.; Lee, Mijoon; Hesek, Dusan; Shi, Qicun; Peng, Jeffrey; Baker, Brian M.; Mobashery, Shahriar (Notre)


    The integral membrane protein BlaR1 of methicillin-resistant Staphylococcus aureus senses the presence of {beta}-lactam antibiotics in the milieu and transduces the information to the cytoplasm, where the biochemical events that unleash induction of antibiotic resistance mechanisms take place. We report herein by two-dimensional and three-dimensional NMR experiments of the sensor domain of BlaR1 in solution and by determination of an x-ray structure for the apo protein that Lys-392 of the antibiotic-binding site is posttranslationally modified by N{sup {zeta}}-carboxylation. Additional crystallographic and NMR data reveal that on acylation of Ser-389 by antibiotics, Lys-392 experiences N{sup {zeta}}-decarboxylation. This unique process, termed the lysine N{sup {zeta}}-decarboxylation switch, arrests the sensor domain in the activated ('on') state, necessary for signal transduction and all the subsequent biochemical processes. We present structural information on how this receptor activation process takes place, imparting longevity to the antibiotic-receptor complex that is needed for the induction of the antibiotic-resistant phenotype in methicillin-resistant S. aureus.

  1. MHC class I ligation of human T cells activates the ZAP70 and p56lck tyrosine kinases, leads to an alternative phenotype of the TCR/CD3 zeta-chain, and induces apoptosis

    Skov, S; Bregenholt, S; Claesson, Mogens Helweg


    that the ZAP70 tyrosine kinase is tyrosine phosphorylated in Jurkat T cells and in purified peripheral T cells after MHC-I ligation. The tyrosine-phosphorylated ZAP70 kinase exhibits a particular phenotype with low affinities for proteins at 21, 40, 60, and 120 kDa, proteins normally co-precipitated with ZAP70...... after TCR/CD3 stimulation. The phosphorylation of ZAP70 after MHC-I ligation was dependent on TCR/CD3 surface expression. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex. MHC-I cross-linking induces a phosphorylated zeta-protein that migrates as a dimer at 42 k...... of the zeta-chain and the ZAP70 kinase following MHC-I ligation. Previous studies have demonstrated that lack or diminished activation of ZAP70 is involved in the induction of anergy or apoptosis in T cells. Likewise, MHC-I cross-linking of Jurkat T cells results in growth arrest and induction of apoptosis...

  2. zeta-, epsilon-, and gamma-Globin mRNA in blood samples and CD71(+) cell fractions from fetuses and from pregnant and nonpregnant women, with special attention to identification of fetal erythroblasts

    Høgh, A M; Hviid, T V; Christensen, B;


    BACKGROUND: Information about the appearance of gamma-, epsilon-, and zeta-globin mRNAs in fetal erythroblasts during gestation and about the presence and amounts of these mRNAs in pregnant and nonpregnant women is important from the perspective of using these molecules as a marker of fetal...... analysis of gamma- and epsilon-globin cDNA, and quantitative analysis of gamma-globin mRNA based on competitive RT-PCR to investigate these aspects. RESULTS: All adult whole-blood samples were negative for epsilon- and zeta-globin mRNA. Analyses of CD71(+) cell fractions showed that specimens from 19 of 20...... nonpregnant and 10 of 14 pregnant women (at 9-13 weeks of gestation) were positive for gamma-globin mRNA (Fisher's exact test, P = 0.13), and those from 3 of 20 nonpregnant and 5 of 14 pregnant women were positive for zeta-globin mRNA (Fisher's exact test, P = 0.23). No epsilon-globin mRNA was detected in CD...

  3. Market, Regulation, Market, Regulation

    Frankel, Christian; Galland, Jean-Pierre


    This paper focuses on the European Regulatory system which was settled both for opening the Single Market for products and ensuring the consumers' safety. It claims that the New Approach and Standardization, and the Global Approach to conformity assessment, which suppressed the last technical...... barriers to trade in Europe, realized the free movement of products by organizing progressively several orders of markets and regulation. Based on historical and institutional documents, on technical publications, and on interviews, this article relates how the European Commission and the Member States had...... alternatively recourse to markets and to regulations, at the three main levels of the New Approach Directives implementation. The article focuses also more specifically on the Medical Devices sector, not only because this New Approach sector has long been controversial in Europe, and has recently been concerned...

  4. Market, Regulation, Market, Regulation

    Frankel, Christian; Galland, Jean-Pierre


    This paper focuses on the European Regulatory system which was settled both for opening the Single Market for products and ensuring the consumers' safety. It claims that the New Approach and Standardization, and the Global Approach to conformity assessment, which suppressed the last technical...... barriers to trade in Europe, realized the free movement of products by organizing progressively several orders of markets and regulation. Based on historical and institutional documents, on technical publications, and on interviews, this article relates how the European Commission and the Member States had...... alternatively recourse to markets and to regulations, at the three main levels of the New Approach Directives implementation. The article focuses also more specifically on the Medical Devices sector, not only because this New Approach sector has long been controversial in Europe, and has recently been concerned...

  5. 细粒棘球绦虫14-3-3zeta蛋白的生物信息学分析%Application of bioinformatic analysis in 14-3-3zeta protein of Echinococcus granulosus

    符瑞佳; 吕刚; 尹飞飞; 梁培


    目的:应用生物信息学技术对细粒棘球绦虫(Echinococcus granulosus)14-3-3zeta蛋白的结构和功能进行预测和分析,为进一步的实验研究提供依据。方法利用美国国家生物技术信息中心(NCBI,和瑞士生物信息学研究所的蛋白分析专家系统(ExPASY,提供的各种有关基因和蛋白序列、结构信息分析的工具,并结合其它生物信息学分析软件,对该蛋白质的结构和功能进行预测和分析。结果该基因全长为771 bp ,编码256个氨基酸,其编码的蛋白相对分子量理论预测值和等电点分别是29.4 kDa和5.04。预测该蛋白无信号肽和跨膜区,二级结构含8个α-螺旋和12个β-折叠股,氨基酸序列中有9个潜在抗原表位。结论初步认识了细粒棘球绦虫14-3-3zeta蛋白的基本特征,为深入研究该蛋白的生物学功能奠定了基础。%Objective To predict and analyze the structure and function of 14-3-3zeta protein from Echinococcus granulosus by bioinformatics technology. Methods The structure and function of Eg14-3-3zeta protein was identified from two biological information sites, USA National Center for Biotechnology Information (NCBI,, and Expert System for analysis of protein of the Swiss Institute of bioinformatics (ExPASY,, which offer the analysis of various related gene and protein sequence, structure information tools, and other bioinformatics analysis software. Results The full-length cDNA sequence encoding Eg14-3-3zeta included a complete open reading frame (ORF) of 771 bp coding to a putative protein with 256 amino acids. Molecular weight of Eg14-3-3zeta was predicted to be 29.4 kDa and its isoelectric point was 5.04. The protein had no signal peptide site and transmembrane do-main. Secondary structure of Eg14-3-3zeta contained 8 alpha-helices and 12 beta-strands.There were

  6. Probing the interaction of human serum albumin with vitamin B2 (riboflavin) and L-Arginine (L-Arg) using multi-spectroscopic, molecular modeling and zeta potential techniques

    Memarpoor-Yazdi, Mina [Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad (Iran, Islamic Republic of); Mahaki, Hanie, E-mail: [Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad (Iran, Islamic Republic of)


    This study was designed to examine the interaction of Riboflavin (RB) and L-Arginine (L-Arg) with human serum albumin (HSA) using different spectroscopic, zeta potential and molecular modeling techniques under imitated physiological conditions. The resonance light scattering (RLS) method determined the critical aggregation concentration of RB on HSA in the presence and absence of L-Arg which confirmed the zeta potential results. The binding constants (K{sub a}) of HSA–RB were 2.5×10{sup 4} and 9.7×10{sup 3} M{sup −1}, respectively in binary and ternary system at the excitation wavelength of 280 nm, also were 7.5×10{sup 3} and 7.3×10{sup 3}, respectively in binary and ternary system at the excitation wavelength of 295 nm. Fluorescence spectroscopy demonstrated that in the presence of L-Arg, the binding constant of HSA–RB was increased. Static quenching was confirmed to results in the fluorescence quenching and FRET. The binding distances between HSA and RB in two- and three-component systems were estimated by the Forster theory which revealed that nonradiative energy transfer from HSA to RB occurred with a high probability. The effect of RB on the conformation of HSA was analyzed using synchronous fluorescence spectroscopy and circular dichroism (CD) in both systems. Docking studies demonstrated a reduction in the binding affinity between RB and HSA in the presence of L-Arg. -- Highlights: ► We studied the interaction of riboflavin with HSA in presence and absence of L-Arg. ► Molecular modeling and zeta-potential used to describe competitive interaction. ► We compared the binding mechanism of riboflavin (RB) to HSA in both systems. ► We determined critical aggregation concentration of RB on HSA in both systems. ► The binding site of RB on HSA in both systems has been determined.

  7. A single-channel method for evaluation of very magnitudes of Ca2+ ion fluxes through epsilon4/zeta1 N-methyl-D-aspartate receptor channels in bilayer lipid membranes.

    Wakabayashi, M; Hirano, A; Sugawara, M; Uchino, S; Nakajima-Iijima, S


    A single-channel method for evaluating agonist selectivity in terms of the very number of Ca2+ ions passed through the epsilon4/zeta1 N-methyl-D-aspartate (NMDA) receptor ion channel in bilayer lipid membranes (BLMs) is described. The number of Ca2+ passed through the single-channel was obtained from single-channel recordings in a medium where the primary permeant ion is Ca2+. The recombinant epsilon4/zeta1 NMDA channel was partially purified from Chinese hamster ovary cells expressing the channel and incorporated in BLMs formed by the tip-dip method. It was found that the epsilon4/zeta1 channel in BLMs is permeable to Ca2+ and Na+, but the number of Ca2+ passed through the channel is much fewer than that of Na+. The integrated Ca2+ currents induced by three typical agonists NMDA, L-glutamate and L-CCG-IV were obtained at concentration of 50 microM, where the integrated currents for all the agonists reached their saturated values. The integrated Ca2+ currents obtained are (3.1+/-0.21) x 10(-13) C/s for NMDA, (4.6+/-0.31) x 10(-13) C/s for L-glutamate and (5.7+/-0.25) x 10(-13) C/s for L-CCG-IV, respectively, suggesting that the three kinds of agonists have different efficacies to induce permeation of Ca2+. The range of the agonist selectivity thus obtained is much narrower than that of binding affinities for the NMDA receptors from rat brain. The present method is able to detect Ca2+ permeation with a detection limit of approximately 10(5) Ca2+ ions/s.

  8. Potencial zeta y energía libre superficial en el proceso de adsorción de cloruro de n-cetilpiridina sobre tejido de poliéster

    Espinosa Jiménez, Manuel; Padilla Weigand, Regina; Ontiveros Ortega, Alfonso; Perea Carpio, Rafael


    Se describe en el presente trabajo un análisis electrocinético y termodinámico del proceso de adsorción de cloruro de n-cetilpiridina (N-CP-Cl) sobre tejido de poliéster.. El estudio electrocinético fue llevado a cabo por medio de movilidad electroforética en el sistema poliéster-tensioactivo. Se encontró un incremento del potencial zeta debido a las atracciones electroestáticas entre los grupos carboxilo de la fibra de poliéster, ionizados a pH=8, y el grupo piridina del tensioactivo. La can...

  9. Roles of integrin αvβ3 in interleukin-8-mediated metastasis and angiogenesis of estrogen receptor negative breast cancer cells%整合素αvβ3在白细胞介素-8介导的雌激素受体阴性乳腺癌细胞侵袭及肿瘤血管生成中的作用

    李李; 邵楠; 叶润仪; 李杰; 王深明; 林颖


    目的 探讨白细胞介素(IL)-8和整合素αvβ3在雌激素受体(ER)阴性乳腺癌细胞侵袭和肿瘤血管生成过程中的相互作用和关系.方法 通过肿瘤细胞侵袭、黏附实验及血管内皮增殖、毛细血管管腔生成实验观察αvβ3在IL-8介导的乳腺癌细胞侵袭及肿瘤血管生成中的作用.应用组织芯片技术,通过免疫组织化学法检测104例乳腺癌患者标本验证IL-8及αvβ3表达与ER的关系.结果 IL-8组BT-549及MDA-MB-231细胞侵袭、黏附的细胞数显著高于对照组(P<0.05),而在IL-8+ αvβ3抗体组显著低于IL-8处理组(P<0.01);MCF-7在3组中侵袭和黏附的细胞数差异无统计学意义(P>0.05).人脐静脉内皮细胞(HUVEC)在24、48、72 h对照组中增殖速度显著低于IL-8组(P<0.01),IL-8+αvβ3抗体组较IL-8组增殖速度显著降低(P<0.01).与对照组比较,IL-8组中HUVEC更易形成类血管样的管状结构排列;而经过αvβ3抗体处理后HUVEC形成的管状结构减少.检测104例乳腺癌标本,αvβ3在IL-8阳性组和阴性组的表达率分别为62.1%和32.0%(P<0.01),其中ER阳性乳腺癌αvβ3的表达率分别为40.0%和26.1% (P>0.05);而ER阴性乳腺癌组织αvβ3的表达率为73.7%和41.4% (P <0.05).结论 ER阴性人乳腺癌细胞株中IL-8促进乳腺癌侵袭和肿瘤血管生成的作用可能与整合素αvβ3有关;IL-8和αvβ3在乳腺癌组织中的表达呈正相关,且与ER状态有关.%Objective To identify the role of integin αvβ3 in interleukin (IL)-8-mediated metastasis and angiogenesis of breast cancer cells.Methods IL-8 and neutralizing antibody of integrin αvβ3 were used in invasion and human umbilical vein endothelial cells (HUVECs)-tumor cells adhesion assay to observe the changes of metastasis of breast cancer cells.Proliferation assay and angiogenesis of HUVECs cultured with IL-8 and antibody of αvβ3 were applied to evaluate the effect of αvβ3 on IL-8-mediated angiogenesis.The correlation between IL-8 and αvβ3 expression was examined by using immunohistochemistry,and relationship between the expression of IL-8 and αvβ3 with estrogen receptor (ER) status in patients was further analyzed.Results IL-8 significantly inhanced the migration of ER negative breast cancer cell lines BT-549 and MDA-MB-231 as compared with those cultured with phosphate buffer (PBS,P<0.05).After the addition of antibody of αvβ3,the number of BT-549 and MDA-MB-231 cells cultured with IL-8 was declined significantly (P <0.01),whereas IL-8 had no effect on ER positive breast cancer cell line MCF-7 (P > 0.05).Proliferation of HUVECs cultured with IL-8 was markedly promoted (P <0.01),which could be blocked by antibody of αvβ3 (P <0.01).HUVECs cultured with IL-8 tended to form more microangioid structure,and suppression of angiogenesis could be observed after the addition of antibody of αvβ3.In the 104 breast cancer specimens,62.1% showed positive αvβ3 expression in the IL-8 positive group,which was significantly higher than that in IL-8 negative group (P < 0.01).In addition,the expression of αvβ3 was correlated with IL-8 in ER-negative breast cancer cells (P < 0.05).Conclusion Integrin αvβ3 may be involved in metastasis and angiogenesis of ER-negative breast cancer cells promoted by IL-8.The expression of αvβ3 correlates with IL-8 expression,which is related to ER status.

  10. A new Vitreoscilla filiformis extract grown on spa water-enriched medium activates endogenous cutaneous antioxidant and antimicrobial defenses through a potential Toll-like receptor 2/protein kinase C, zeta transduction pathway

    Mahe YF


    Full Text Available Yann F Mahe,1 Marie-Jesus Perez,1 Charlotte Tacheau,1 Chantal Fanchon,2 Richard Martin,3 Françoise Rousset,1 Sophie Seite4 1L’Oreal Research and Innovation, Clichy, 2L’Oréal Research and Innovation, Chevilly Larue, 3L’Oréal Research and Innovation Tours, 4La Roche-Posay Pharmaceutical Laboratories, Asnières, France Abstract: Vitreoscilla filiformis (VF biomass (VFB has been widely used in cosmetic preparations and shown to modulate the major inducible free-radical scavenger mitochondrial superoxide dismutase in skin cells. By adding La Roche-Posay (LRP thermal spring water to the VF culture medium, we obtained a biomass (LRP-VFB with a similar mitochondrial superoxide dismutase activation capacity to VF. Also, the new biomass more powerfully stimulated mRNA expression and antimicrobial peptides in reconstructed epidermis. Interestingly, a predictive computer model that analyzed transducing events within skin epidermal cells suggested that this protective activity may involve the Toll-like receptor 2/protein kinase C, zeta transduction pathway. Protein kinase C, zeta inhibition was effectively shown to abolish VFB-induced gene stimulation and confirmed this hypothesis. This thus opens new avenues for investigation into the improvement of skin homeostatic defense in relation to the control of its physiological microbiota and innate immunity. Keywords: innate skin defenses, TLR2, PKCz, La Roche-Posay, mitochondrial superoxide dismutase, SOD2

  11. Mapping Protein–Protein Interactions of the Resistance-Related Bacterial Zeta Toxin–Epsilon Antitoxin Complex (ε2ζ2 with High Affinity Peptide Ligands Using Fluorescence Polarization

    María Isabel Fernández-Bachiller


    Full Text Available Toxin–antitoxin systems constitute a native survival strategy of pathogenic bacteria and thus are potential targets of antibiotic drugs. Here, we target the Zeta–Epsilon toxin–antitoxin system, which is responsible for the stable maintenance of certain multiresistance plasmids in Gram-positive bacteria. Peptide ligands were designed on the basis of the ε2ζ2 complex. Three α helices of Zeta forming the protein–protein interaction (PPI site were selected and peptides were designed conserving the residues interacting with Epsilon antitoxin while substituting residues binding intramolecularly to other parts of Zeta. Designed peptides were synthesized with an N-terminal fluoresceinyl-carboxy-residue for binding assays and provided active ligands, which were used to define the hot spots of the ε2ζ2 complex. Further shortening and modification of the binding peptides provided ligands with affinities <100 nM, allowing us to determine the most relevant PPIs and implement a robust competition binding assay.

  12. Regulating Transplants


    Legislation to determine brain death is viewed as essential in controlling the organ transplant industry Organ transplant represents a very sensitive and complicated issue. Experts say the temporary administrative regulations recently promulgated by the Central Government are an important step, but relevant laws and regulations must follow. Among these, the

  13. Applications of zeta functions and other spectral functions in mathematics and physics: a special issue in honour of Stuart Dowker's 75th birthday Applications of zeta functions and other spectral functions in mathematics and physics: a special issue in honour of Stuart Dowker's 75th birthday

    Dowker, Fay; Elizalde, Emilio; Kirsten, Klaus


    . Chapter 5 is devoted to the image and inversion methods and I must have read this closely as there are lots of marginal notes...' The second principle can be read off from the following quote: 'After reading Eddington circa 1960 it was clear to me (and others of course) there is a strong analogy (at least) between gravitation and electromagnetism ... (His work has very strongly influenced me.) So I played a game of asking for the gravitational analogues of existing electromagnetic concepts. The basic analogue is between field strength/charge and curvature/spin... [in that] ...spin, in general relativity, plays the passive role that charge plays in electromagnetism in the sense that it is the spin-curvature coupling that knocks a particle off a geodesic.' It is quite amazing how much of Stuart's work can be traced back to these principles. This is briefly explained in the following by describing some of his most important works. His most cited work [7] fits this bill and can be seen as doing what Julian Schwinger did in [8], for a constant electromagnetic field in the gravitational setting. In more detail he noticed Schulman's work on propagators on the three sphere [9], which he extended to Lie groups; see [10, 11]. He then noticed that having exact propagators, work like Schwinger's could be done and de Sitter space was a natural 'curved' candidate. The paper is best known for the mathematical technique introduced, namely for the zeta function method much used since for the computation of singular quantum field theoretic quantities like effective actions and the Casimir energy. The motivation for introducing this scheme goes back to reading an article of I M Gel'fand about some number theory problem involving zeta functions, image sums, propagators etc. How could an object occurring with other objects physicists were using all the time not be useful? It turned out to be very useful, although in this paper the method was only introduced but actually not used! This is

  14. Raw Coal Density Affected to Surface Zeta Potential of Muddy and Slime Particle%原煤密度对泥化及煤泥颗粒表面ξ电位的影响

    赵晴; 闵凡飞; 刘令云; 李宏亮


    The muddy experiment on 50 -0.5 mm different density raw coal from Dingji Coal Preparation Plant of Huainan Mining Area was conducted. The X-ray diffractor and the electrophoresis experiment was individually applied to measure the raw coal mineral composition and the zeta potential on the micro particle surface of the slime. The results showed that the main mineral composition of the raw coal to be prepared would be including quartz, kaolinite, green mudstone and others. The muddy rate of the high density and medium density raw coal was high. In the muddy slime of the +1. 60 g/cmJ raw coal, the -0. 045 mm particles would be the majority and the -0. 025 mm micro particles would be over 90%. With the raw coal density increased and the slime particle reduced, the zeta potential on the micro particle surface of the muddy slime would be reduced in potential. The water hardness, the soluble salt in the raw coal and the particle surface property would have important influence to the zeta potential occurred on the slime micro particle. The zeta potential increased on the slime micro particle surface would cause the particles in the slime water in more stable dispersed state.%对淮南矿区丁集选煤厂50~0.5 mm不同密度级原煤进行了泥化试验,采用X-射线衍射 仪和电泳试验分别对原煤矿物组成和煤泥微细颗粒表面ξ电位进行了测定.结果表明:入选原煤中主要矿物成分有石英、高岭石、绿泥石等;高密度和中间密度级原煤泥化率大,+1.60g/cm3原煤泥化煤泥中-0.045 mm颗粒最多,其中约90%以上为-0.025 mm的微细颗粒;随着原煤密度的增大和煤泥粒度的减小,泥化煤泥微细颗粒表面ξ电位呈减小趋势,水的硬度、原煤中可溶性盐及颗粒表面性质对煤泥微细颗粒ξ电位产生重要影响,微细煤泥颗粒表面ξ电位增大会导致其在煤泥水中处于更加稳定的分散状态.

  15. On the Unusual Homeoviscous Adaptation of the Membrane Fatty Acyl Components against the Thermal Stress in Rhi{Zeta}obium meliloti

    Kang, Seb Yung; Jung, Seun Ho [Konkuk University, Seoul (Korea, Republic of); Choi, Yong Hoon; Yang, Chul Hak [Seoul National University, Seoul (Korea, Republic of); Kim, Hyun Won [Yonsei University Wonju College of Medicine, Wonju (Korea, Republic of)


    In order to maintain the optimal fluidity in membrane, microorganism genetically regulates the ratio of the unsaturated fatty acids (Ufos) to saturated ones of its biological membrane in response to external perturbing condition such as the change of temperature. The remodelling of fatty acyl chain composition is the most frequently observed response to altered growth temperature. It is reflected in the elevated proportions of unsaturated fatty acid (UFAs) at low temperature. Because cis double bonds, normally positioned at the middle of fatty acyl chains, introduce a kink of approximately 30 .deg. into acyl chain, UFAs pack less compactly and exhibit lower melting points than their saturated homologues. Thus, enrichment of membranes with UFAs offsets, to a significant degree, the increase in membrane order caused by a drop in temperature. This is so called homeoviscous adaptation of the membrane fatty acyl chains against thermal stress. Membrane maintains the optimal viscosity using homeoviscous adaptation.

  16. NORM regulations

    Gray, P. [ed.


    The author reviews the question of regulation for naturally occuring radioactive material (NORM), and the factors that have made this a more prominent concern today. Past practices have been very relaxed, and have often involved very poor records, the involvment of contractors, and the disposition of contaminated equipment back into commercial service. The rationale behind the establishment of regulations is to provide worker protection, to exempt low risk materials, to aid in scrap recycling, to provide direction for remediation and to examine disposal options. The author reviews existing regulations at federal and state levels, impending legislation, and touches on the issue of site remediation and potential liabilities affecting the release of sites contaminated by NORM.

  17. Exo1 phosphorylation status controls the hydroxyurea sensitivity of cells lacking the Pol32 subunit of DNA polymerases delta and zeta.

    Doerfler, Lillian; Schmidt, Kristina H


    Exo1 belongs to the Rad2 family of structure-specific nucleases and possesses 5'-3' exonuclease activity on double-stranded DNA substrates. Exo1 interacts physically with the DNA mismatch repair (MMR) proteins Msh2 and Mlh1 and is involved in the excision of the mispaired nucleotide. Independent of its role in MMR, Exo1 contributes to long-range resection of DNA double-strand break (DSB) ends to facilitate their repair by homologous recombination (HR), and was recently identified as a component of error-free DNA damage tolerance pathways. Here, we show that Exo1 activity increases the hydroxyurea sensitivity of cells lacking Pol32, a subunit of DNA polymerases δ and ζ. Both, phospho-mimicking and dephospho-mimicking exo1 mutants act as hypermorphs, as evidenced by an increase in HU sensitivity of pol32Δ cells, suggesting that they are trapped in an active form and that phosphorylation of Exo1 at residues S372, S567, S587, S692 is necessary, but insufficient, for the accurate regulation of Exo1 activity at stalled replication forks. In contrast, neither phosphorylation status is important for Exo1's role in MMR or in the suppression of genome instability in cells lacking Sgs1 helicase. This ability of an EXO1 deletion to suppress the HU hypersensitivity of pol32Δ cells is in contrast to the negative genetic interaction between deletions of EXO1 and POL32 in MMS-treated cells as well as the role of EXO1 in DNA-damage treated rad53 and mec1 mutants.

  18. Ionic Distribution, Electrostatic Potential and Zeta Potential at Electrochemical Interfaces%电化学界面处离子分布、静电势和ζ势的研究

    于养信; 吴建中; 高光华


    Density functional theory is applied to predicting the structures and electrostatic potentials of planar electrochemical surfaces within the framework of the restricted primitive model where small ions are represented by charged hard spheres of equal diameter and the solvent is assumed to be a continuous dielectric medium. The hard-sphere contribution to the excess Helmholtz energy functional is evaluated using the modified fundamentalmeasure theory and the electrostatic contribution is obtained from the quadratic functional Taylor expansion using the second-order direct correlation function from the mean-spherical approximation. Numerical results for the ionic density profiles and the mean electrostatic potentials near a planar surface of various charge densities are in excellent agreement with molecular simulations. In contrast to the modified Gouy-Chapman theory, the present density functional theory correctly predicts the second layer formation and charge inversion of charged surfaces as observed in simulations and in experiments. The theory has also been tested with the zeta potentials of positively charged polystyrene particles in aqueous solutions of KBr. Good agreement is achieved between the calculated and experimental results.

  19. ROZA-XL, an improved FRET based biosensor with an increased dynamic range for visualizing zeta associated protein 70 kD (ZAP-70) tyrosine kinase activity in live T cells.

    Cadra, Sophie; Gucciardi, Alexia; Valignat, Marie-Pierre; Theodoly, Olivier; Vacaflores, Aldo; Houtman, Jon C D; Lellouch, Annemarie C


    Genetically encoded FRET based biosensors allow one to visualize the spatial and temporal evolution of specific enzyme activities in live cells. We have previously reported the creation of a FRET based biosensor specific for Zeta-Associated Protein -70 kD (ZAP-70) (Randriamampita et al., 2008), a Syk family protein tyrosine kinase. ZAP-70 is essential for early T cell receptor (TCR) signaling events, T lymphocyte development and has also been implicated in integrin mediated T lymphocyte migration. In order to facilitate the study of ZAP-70 kinase activity during dynamic phenomena such as immunological synapse formation or cell migration, we have designed and prepared a second generation of ZAP-70 specific biosensors. Here we describe a novel biosensor named ROZA-XL, that displays a 3-4 times greater dynamic range than its predecessor and possesses a robust baseline FRET value when expressed in the Jurkat human T cell line. We demonstrate that the robust behavior of this biosensor allows for rapid analysis of TCR mediated of ZAP-70 kinase activity at a single cell level, as shown in a simple end point assay in which ROZA-XL expressing cells are allowed to interact with stimulatory anti-CD3epsilon coated coverslips.

  20. Pleiotrophin Regulates the Retention and Self-Renewal of Hematopoietic Stem Cells in the Bone Marrow Vascular Niche

    Heather A. Himburg


    Full Text Available The mechanisms through which the bone marrow (BM microenvironment regulates hematopoietic stem cell (HSC fate remain incompletely understood. We examined the role of the heparin-binding growth factor pleiotrophin (PTN in regulating HSC function in the niche. PTN−/− mice displayed significantly decreased BM HSC content and impaired hematopoietic regeneration following myelosuppression. Conversely, mice lacking protein tyrosine phosphatase receptor zeta, which is inactivated by PTN, displayed significantly increased BM HSC content. Transplant studies revealed that PTN action was not HSC autonomous, but rather was mediated by the BM microenvironment. Interestingly, PTN was differentially expressed and secreted by BM sinusoidal endothelial cells within the vascular niche. Furthermore, systemic administration of anti-PTN antibody in mice substantially impaired both the homing of hematopoietic progenitor cells to the niche and the retention of BM HSCs in the niche. PTN is a secreted component of the BM vascular niche that regulates HSC self-renewal and retention in vivo.

  1. Prime pairs and the zeta function

    Korevaar, J.


    Are there infinitely many prime pairs with given even difference? Most mathematicians think so. Using a strong arithmetic hypothesis, Goldston, Pintz and Yildirim have recently shown that there are infinitely many pairs of primes differing by at most sixteen. There is extensive numerical support for

  2. PKM[zeta] Inactivation Induces Spatial Familiarity

    Moncada, Diego; Viola, Haydee


    Spatial familiarization consists of a decrease in the exploratory activity over time after exposure to a place. Here, we show that a 30-min exposure to an open field led to a pronounced decrease in the exploratory behavior of rats, generating context familiarity. This behavioral output is associated with a selective decrease in hippocampal…

  3. Prime pairs and the zeta function

    Korevaar, J.


    Are there infinitely many prime pairs with given even difference? Most mathematicians think so. Using a strong arithmetic hypothesis, Goldston, Pintz and Yildirim have recently shown that there are infinitely many pairs of primes differing by at most sixteen. There is extensive numerical support for

  4. 77 FR 72975 - Zeta Cypermethrin; Pesticide Tolerances


    ... = 11.20 mg/ old and adults). mg/kg. day. kg based on motor UFA = 10x aPAD = 0.07 mg/kg/day.. activity... the rat. LOAEL = HED = 1.15 mg/kg/day... 0.05 mg/L based on UFA = 3x increased salivation. UFH = 10x... = uncertainty factor. UFA = extrapolation from animal to human (interspecies). UFH = potential variation...

  5. Association between Polymorphism of Phospholipase C-zeta Gene (PLCz)and Sperm Quality Traits in Chinese Holstein Bulls%磷脂酶C-zeta基因(PLCz)多态性与中国荷斯坦公牛精液性状相关性研究

    潘晴; 鞠志花; 张燕; 黄金明; 李建斌; 王玲玲; 齐超; 侯明海; 仲跻峰


    The testis-specific phospholipase C isoform, PLC-zeta (PLCz), is widely considered to be a quite essential role in the oocyte activation and embryo development owing to its ability to trigger intracellular a series of Ca2+ oscillations in all mammals. Recently, while PLCz as the molecular trigger for development of a fertilised egg into an embryo has been reported, the exact molecular mechanism for generating Ca2+ oscillations and the PLCz gene expression and regulation has not been established. Accordingly, the objectives of this study were to clone and characterize the expression and regulation of bovine (Bos taurus) PLCz in sperm through the analysis of single nucleotide polymorphisms (SNPs) using DNA sequencing methods and PCR-restriction fragment length polymorphism (PCR-RFLP) to study their association with sperm quality and fertility of 424 normal Chinese Holstein Bulls from three bull stations. Molecular cloning of the bovine PLCz yielded a 53 160 bp sequence which transcripted into a 2 176 bp codons and encoded a number of 634 amino acid. We detected eight novel SNPs, g.27529 T>A and g.27597 T>C located in the seventh intron, g.47969 G>A, g.48020 T>C, g.48079 G>A, g.48127 G>T and g.48384 G>T in the twelveth intron, thus the SNP g.27768 G>C in the eighth exon was identified as a non-synonymous mutation [GCC(Ala)>CCC (Pro)] at 337th amino acid. Significantly, linkage disequilibrium analysis showed that g.27529 T>A, g.27597 T>C and g.27768 G>C (SNP1); g.47969 G>A, g.48020 T>C, g.48079 G>A, g.48127 G>T and g.48384 G>T (SNP2) were completely linked. Correlation analysis with the sperm quality traits showed that the SNP2 marker had a respective effect on the percentages of viable sperm (P0.05). In addition, The association analysis between nine combined haplotypes and bull reproductive traits showed that the bulls with the combined haplotype TTAB were significantly higher with ejaculate volume (P<0.05), sperm density(P<0.01) and frozen semen motility (P<0

  6. Variance in total levels of phospholipase C zeta (PLC-ζ) in human sperm may limit the applicability of quantitative immunofluorescent analysis as a diagnostic indicator of oocyte activation capability.

    Kashir, Junaid; Jones, Celine; Mounce, Ginny; Ramadan, Walaa M; Lemmon, Bernadette; Heindryckx, Bjorn; de Sutter, Petra; Parrington, John; Turner, Karen; Child, Tim; McVeigh, Enda; Coward, Kevin


    To examine whether similar levels of phospholipase C zeta (PLC-ζ) protein are present in sperm from men whose ejaculates resulted in normal oocyte activation, and to examine whether a predominant pattern of PLC-ζ localization is linked to normal oocyte activation ability. Laboratory study. University laboratory. Control subjects (men with proven oocyte activation capacity; n = 16) and men whose sperm resulted in recurrent intracytoplasmic sperm injection failure (oocyte activation deficient [OAD]; n = 5). Quantitative immunofluorescent analysis of PLC-ζ protein in human sperm. Total levels of PLC-ζ fluorescence, proportions of sperm exhibiting PLC-ζ immunoreactivity, and proportions of PLC-ζ localization patterns in sperm from control and OAD men. Sperm from control subjects presented a significantly higher proportion of sperm exhibiting PLC-ζ immunofluorescence compared with infertile men diagnosed with OAD (82.6% and 27.4%, respectively). Total levels of PLC-ζ in sperm from individual control and OAD patients exhibited significant variance, with sperm from 10 out of 16 (62.5%) exhibiting levels similar to OAD samples. Predominant PLC-ζ localization patterns varied between control and OAD samples with no predictable or consistent pattern. The results indicate that sperm from control men exhibited significant variance in total levels of PLC-ζ protein, as well as significant variance in the predominant localization pattern. Such variance may hinder the diagnostic application of quantitative PLC-ζ immunofluorescent analysis. Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  7. Probing the interaction of human serum albumin with bilirubin in the presence of aspirin by multi-spectroscopic, molecular modeling and zeta potential techniques: insight on binary and ternary systems.

    Hosainzadeh, Akram; Gharanfoli, Mohsen; Saberi, Mohammad; Chamani, JamshidKhan


    Here, we report on the effect of aspirin (ASA), on the binding parameters with regard to bilirubin (BR) to human serum albumin (HSA). Two different classes of binding sites were detected. Binding to the first and second classes of the binding sites was dominated by hydrophobic forces in the case of HSA-BR, whereas in the case of the ternary system, binding to the first and second classes of the binding sites was achieved by electrostatic interaction. The binding constant (K(a)) and number of binding site (n) obtained were 1.6 × 10(6)M(-1) and 0.98, respectively, for the primary binding site in the case of HSA-BR, and 3.7 × 10(6)M(-1) and 0.84, respectively, in the presence of ASA (ternary complex) at λ(ex)= 280 nm. The progressive quenching of the protein fluorescence as the BR concentration increased indicated an arrangement of the domain IIA in HSA. Changes in the environment of the aromatic residues were also observed by synchronous fluorescence spectroscopy (SFS). Changes of the secondary structure of HSA involving a decrease of α-helical and β-sheet contents and increased amounts of turns and unordered conformations were mainly found at high concentrations of BR. For the first time, the relationship between the structural parameters of HSA-BR by RLS for determining the critical induced aggregation concentration (C(CIAC)) of BR in the absence and presence of ASA was investigated, and there was a more significant enhancement in the case of the ternary mixture as opposed to the binary one. Changes in the zeta potential of HSA and the HSA-ASA complex in the presence of BR demonstrated a hydrophobic adsorption of this anionic ligand onto the surface of HSA in the binary system as well as both electrostatic and hydrophobic adsorption in the case of the ternary complex. By performing docking experiments, it was found that the acting forces between BR and HSA were mainly hydrophobic > hydrogen bonding > electrostatic interactions, and consequently BR had a long

  8. Induction of neutrophil gelatinase-associated lipocalin expression by co-stimulation with interleukin-17 and tumor necrosis factor-alpha is controlled by IkappaB-zeta but neither by C/EBP-beta nor C/EBP-delta

    Karlsen, Joachim R; Borregaard, Niels; Cowland, Jack B


    Neutrophil gelatinase-associated lipocalin (NGAL) is a siderophore-binding antimicrobial protein that is up-regulated in epithelial tissues during inflammation. We demonstrated previously that the gene encoding NGAL (LCN2) is strongly up-regulated by interleukin (IL)-1beta in an NF-kappaB-depende...

  9. Combinatorial Gene Regulation Using Auto-Regulation

    Hermsen, Rutger; Ursem, Bas; ten Wolde, Pieter Rein


    As many as 59% of the transcription factors in Escherichia coli regulate the transcription rate of their own genes. This suggests that auto-regulation has one or more important functions. Here, one possible function is studied. Often the transcription rate of an auto-regulator is also controlled by additional transcription factors. In these cases, the way the expression of the auto-regulator responds to changes in the concentrations of the “input” regulators (the response function) is obviously affected by the auto-regulation. We suggest that, conversely, auto-regulation may be used to optimize this response function. To test this hypothesis, we use an evolutionary algorithm and a chemical–physical model of transcription regulation to design model cis-regulatory constructs with predefined response functions. In these simulations, auto-regulation can evolve if this provides a functional benefit. When selecting for a series of elementary response functions—Boolean logic gates and linear responses—the cis-regulatory regions resulting from the simulations indeed often exploit auto-regulation. Surprisingly, the resulting constructs use auto-activation rather than auto-repression. Several design principles show up repeatedly in the simulation results. They demonstrate how auto-activation can be used to generate sharp, switch-like activation and repression circuits and how linearly decreasing response functions can be obtained. Auto-repression, on the other hand, resulted only when a high response speed or a suppression of intrinsic noise was also selected for. The results suggest that, while auto-repression may primarily be valuable to improve the dynamical properties of regulatory circuits, auto-activation is likely to evolve even when selection acts on the shape of response function only. PMID:20548950

  10. Trout Stream Special Regulations

    Minnesota Department of Natural Resources — This layer shows Minnesota trout streams that have a special regulation as described in the 2006 Minnesota Fishing Regulations. Road crossings were determined using...

  11. Hepcidin: regulation of the master iron regulator

    Rishi, Gautam; Wallace, Daniel F.; Subramaniam, V. Nathan


    Iron, an essential nutrient, is required for many diverse biological processes. The absence of a defined pathway to excrete excess iron makes it essential for the body to regulate the amount of iron absorbed; a deficiency could lead to iron deficiency and an excess to iron overload and associated disorders such as anaemia and haemochromatosis respectively. This regulation is mediated by the iron-regulatory hormone hepcidin. Hepcidin binds to the only known iron export protein, ferroportin (FPN), inducing its internalization and degradation, thus limiting the amount of iron released into the blood. The major factors that are implicated in hepcidin regulation include iron stores, hypoxia, inflammation and erythropoiesis. The present review summarizes our present knowledge about the molecular mechanisms and signalling pathways contributing to hepcidin regulation by these factors. PMID:26182354

  12. General Theories of Regulation

    Hertog, J.A. den


    This chapter makes a distinction between three types of theories of regulation: public interest theories, the Chicago theory of regulation and the public choice theories. The Chicago theory is mainly directed at the explanation of economic regulation; public interest theories and public choice theor

  13. Spreading depression induces expression of calcium-independent protein kinase C subspecies in ischaemia-sensitive cortical layers: regulation by N-methyl-D-aspartate receptors and glucocorticoids.

    Koponen, S; Keinänen, R; Roivainen, R; Hirvonen, T; Närhi, M; Chan, P H; Koistinaho, J


    Spreading depression is a wave of sustained depolarization challenging the energy metabolism of the cells without causing irreversible damage. In the ischaemic brain, sreading depression-like depolarization contributes to the evolution of ischaemia to infarction. The depolarization is propagated by activation of N-methyl-D-aspartate receptors, but changes in signal transduction downstream of the receptors are not known. Because protein phosphorylation is a general mechanism whereby most cellular processes are regulated, and inhibition of N-methyl-D-aspartate receptors or protein kinase C is neuroprotective, the expression of protein kinase C subspecies in spreading depression was examined. Cortical treatment with KCl induced an upregulation of protein kinase Cdelta and zeta messenger RNA at 4 and 8 h, whereas protein kinase Calpha, beta, gamma and epsilon did not show significant changes. The gene induction was the strongest in layers 2 and 3, and was followed by an increased number of protein kinase Cdelta-immunoreactive neurons. Protein kinase Cdelta and zeta inductions were inhibited by pretreatment with an N-methyl-D-aspartate receptor antagonist, dizocilpine maleate, which also blocked spreading depression propagation, and with dexamethasone, which acted without blocking the propagation. Quinacrine, a phospholipase A2 inhibitor, reduced only protein kinase C5 induction. In addition, N(G)(-nitro-L-arginine methyl ester, a nitric oxide synthase inhibitor, did not influence protein kinase Cdelta or zeta induction, whereas 6-nitro-7-sulphamoylbenzo[f]quinoxaline-2,3-dione, an alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate/kainate receptor antagonist, and the cyclo-oxygenase inhibitors indomethacin and diclophenac tended to increase gene expression. The data show that cortical spreading depression induces Ca2(+)-independent protein kinase C subspecies delta and zeta, but not Ca(2+)-dependent subspecies, through activation of N-methyl-D-aspartate receptors and

  14. Glutathione transferase supergene family in tomato: Salt stress-regulated expression of representative genes from distinct GST classes in plants primed with salicylic acid.

    Csiszár, Jolán; Horváth, Edit; Váry, Zsolt; Gallé, Ágnes; Bela, Krisztina; Brunner, Szilvia; Tari, Irma


    A family tree of the multifunctional proteins, glutathione transferases (GSTs, EC was created in Solanum lycopersicum based on homology to known Arabidopsis GSTs. The involvement of selected SlGSTs was studied in salt stress response of tomato primed with salicylic acid (SA) or in un-primed plants by real-time qPCR. Selected tau GSTs (SlGSTU23, SlGSTU26) were up-regulated in the leaves, while GSTs from lambda, theta, dehydroascorbate reductase and zeta classes (SlGSTL3, SlGSTT2, SlDHAR5, SlGSTZ2) in the root tissues under salt stress. Priming with SA exhibited a concentration dependency; SA mitigated the salt stress injury and caused characteristic changes in the expression pattern of SlGSTs only at 10(-4) M concentration. SlGSTF4 displayed a significant up-regulation in the leaves, while the abundance of SlGSTL3, SlGSTT2 and SlGSTZ2 transcripts were enhanced in the roots of plants primed with high SA concentration. Unexpectedly, under high salinity the SlDHAR2 expression decreased in primed roots as compared to the salt-stressed plants, however, the up-regulation of SlDHAR5 isoenzyme contributed to the maintenance of DHAR activity in roots primed with high SA. The members of lambda, theta and zeta class GSTs have a specific role in salt stress acclimation of tomato, while SlGSTU26 and SlGSTF4, the enzymes with high glutathione conjugating activity, characterize a successful priming in both roots and leaves. In contrast to low concentration, high SA concentration induced those GSTs in primed roots, which were up-regulated under salt stress. Our data indicate that induction of GSTs provide a flexible tool in maintaining redox homeostasis during unfavourable conditions. Copyright © 2014 Elsevier Masson SAS. All rights reserved.


    J. GRAF


    This paper proposes a model relationship between the operator engaged in a hazardous activity, the regulator of that activity, and the general public. The roles and responsibilities of each entity are described in a way that allows effective communication flow. The role of the regulator is developed using the steam boiler as an example of a hazard subject to regulation; however, the model applies to any regulated activity. In this model the safety analyst has the extremely important role of communicating sometimes difficult technical information to the regulator in a way that the regulator can provide credible assurance to the general public as to the adequacy of the control of the hazardous activity. The conclusion asserts that acceptance of the model, understanding of the roles and responsibilities and definition of who communicates what information to whom will mitigate frustration on the part of each of the three entities.

  16. Benchmarking and Regulation

    Agrell, Per J.; Bogetoft, Peter

    nchmarking methods, and in particular Data Envelopment Analysis (DEA), have become well-established and informative tools for economic regulation. DEA is now routinely used by European regulators to set reasonable revenue caps for energy transmission and distribution system operators....... The application of benchmarking in regulation, however, requires specific steps in terms of data validation, model specification and outlier detection that are not systematically documented in open publications, leading to discussions about regulatory stability and economic feasibility of these techniques....... In this paper, we review the modern foundations for frontier-based regulation and we discuss its actual use in several jurisdictions....

  17. Reconceptualizing Civil Regulation

    Galang, Roberto Martin; Castello, Itziar


    This article re-conceptualizes the notion of civil regulation, through an analysis of 775 projects by firms located in 21 Asian countries, wherein we map the state of civil regulation initiatives in the region. We challenge two established assumptions in the Corporate Social Responsibility...... literature. First, contrary to what is commonly argued, we claim that strong states in Asia promote civil regulation in what we call the “paradox of the weak state”. Second, we not only argue that civil regulation is mainly enforced by multinational enterprises willing to promote international social...

  18. Novel regulators of spermatogenesis.

    Fok, Kin Lam; Chen, Hao; Ruan, Ye Chun; Chan, Hsiao Chang


    Spermatogenesis is a multistep process that supports the production of millions of sperm daily. Understanding of the molecular mechanisms that regulate spermatogenesis has been a major focus for decades. Yet, the regulators involved in different cellular processes of spermatogenesis remain largely unknown. Human diseases that result in defective spermatogenesis have provided hints on the molecular mechanisms regulating this process. In this review, we have summarized recent findings on the function and signaling mechanisms of several genes that are known to be associated with disease or pathological processes, including CFTR, CD147, YWK-II and CT genes, and discuss their potential roles in regulating different processes of spermatogenesis.

  19. Plant Growth Regulators.

    Nickell, Louis G.


    Describes the effect of "plant growth regulators" on plants, such as controlling the flowering, fruit development, plant size, and increasing crop yields. Provides a list of plant growth regulators which includes their chemical, common, and trade names, as well as their different use(s). (GA)

  20. Mortgage market regulation: Europe

    Aalbers, M.B.; Smith, S.J.


    Despite several European Union (EU) initiatives, there is only limited pan-European mortgage market regulation. The EU strategy can be characterised as one of parallel liberalisation and consolidation. This article highlights the key differences in regulation among European mortgage markets.

  1. Regulation of SUMO Modification

    P.M. Knipscheer (Puck Maria)


    textabstractThe small ubiquitin related modifier SUMO is a posttranslational modifier that functions in a wide range of cellular processes like intracellular transport, cell cycle regulation, DNA repair and regulation of transcription. SUMO is an 11 kDa protein and is ligated to its target proteins

  2. Mitochondrial glutathione transferases involving a new function for membrane permeability transition pore regulation.

    Aniya, Yoko; Imaizumi, Naoki


    The mitochondria in mammalian cells are a predominant resource of reactive oxygen species (ROS), which are produced during respiration-coupled oxidative metabolism or various chemical stresses. End-products from membrane-lipid peroxidation caused by ROS are highly toxic, thereby their elimination/scavenging are protective of mitochondria and cells against oxidative damages. In mitochondria, soluble (kappa, alpha, mu, pi, zeta) and membrane-bound glutathione transferases (GSTs) (MGST1) are distributed. Mitochondrial GSTs display both glutathione transferase and peroxidase activities that detoxify such harmful products through glutathione (GSH) conjugation or GSH-mediated peroxide reduction. Some GST isoenzymes are induced by oxidative stress, an adaptation mechanism for the protection of cells from oxidative stress. Membrane-bound MGST1 is activated through the thiol modification in oxidative conditions. Protective action of MGST1 against oxidative stress has been confirmed using MCF7 cells highly expressed of MGST1. In recent years, mitochondria have been recognized as a regulator of cell death via both apoptosis and necrosis, where oxidative stress-induced alteration of the membrane permeability is an important step. Recent studies have shown that MGST1 in the inner mitochondrial membrane could interact with the mitochondrial permeability transition (MPT) regulator proteins, such as adenine nucleotide translocator (ANT) and/or cyclophilin D, and could contribute to oxidant-induced MPT pores. Interaction of GST alpha with ANT has also been shown. In this review, functions of the mitochondrial GSTs, including a new role for mitochondria-mediated cell death, are described.

  3. Public regulators and CSR

    Buhmann, Karin


    for responsible business conduct, connecting to social expectations and bridging to public regulation. This UN guidance has had a significant bearing on how public regulators seek to influence business conduct beyond Human Rights to broader Corporate Social Responsibility (CSR) concerns. Drawing on examples...... of such public regulatory governance, this article explores and explains developments towards a juridification of CSR entailing efforts by public regulators to reach beyond jurisdictional and territorial limitations of conventional public law to address adverse effects of transnational economic activity. Through...

  4. Epigenetic Regulation of Adipokines

    Tho X. Pham


    Full Text Available Adipose tissue expansion in obesity leads to changes in the expression of adipokines, adipocyte-specific hormones that can regulate whole body energy metabolism. Epigenetic regulation of gene expression is a mechanism by which cells can alter gene expression through the modifications of DNA and histones. Epigenetic mechanisms, such as DNA methylation and histone modifications, are intimately tied to energy metabolism due to their dependence on metabolic intermediates such as S-adenosylmethionine and acetyl-CoA. Altered expression of adipokines in obesity may be due to epigenetic changes. The goal of this review is to highlight current knowledge of epigenetic regulation of adipokines.

  5. Volume regulation in epithelia

    Larsen, Erik Hviid; Hoffmann, Else Kay


    function of iso-osmotic fluid transport that depends on Na+ recirculation. The causative relationship is discussed for a fluid-absorbing and a fluid-secreting epithelium of which the Na+ recirculation mechanisms have been identified. A large number of transporters and ion channels involved in cell volume...... regulation are cloned. The volume-regulated anion channel (VRAC) exhibiting specific electrophysiological characteristics seems exclusive to serve cell volume regulation. This is contrary to K+ channels as well as cotransporters and exchange mechanisms that may serve both transepithelial transport and cell...

  6. Electrical installations and regulations

    Whitfield, J F


    Electrical Installations and Regulations focuses on the regulations that apply to electrical installations and the reasons for them. Topics covered range from electrical science to alternating and direct current supplies, as well as equipment for providing protection against excess current. Cables, wiring systems, and final subcircuits are also considered, along with earthing, discharge lighting, and testing and inspection.Comprised of 12 chapters, this book begins with an overview of electrical installation work, traits of a good electrician, and the regulations governing installations. The r

  7. Sport Fishing Regulations

    US Fish and Wildlife Service, Department of the Interior — The regulations for sport fishing on St. Vincent National Wildlife Refuge are outlined in this document. Fishing is only permitted from sunrise to sunset, and only...

  8. Focus on PTEN regulation

    Miriam eBermudez-Brito


    Full Text Available The role of PTEN as a tumour suppressor has been for a long time attributed to its lipid phosphatase activity against PI(3,4,5P3, the phospholipid product of the class I PI3Ks. Besides its traditional role as a lipid phosphatase at the plasma membrane, a wealth of data has shown that PTEN can function independently of its phosphatase activity and that PTEN also exists and plays a role in the nucleus, in cytoplasmic organelles and extracellularly. Accumulating evidence has shed light on diverse physiological functions of PTEN which are accompanied by a complex regulation of its expression and activity. PTEN levels and function are regulated transcriptionally, post-transcriptionally and post-translationally. PTEN is also sensitive to regulation by its interacting proteins and its localization. Herein, we summarize the current knowledge on mechanisms that regulate the expression and enzymatic activity of PTEN and its role in human diseases.

  9. Volume Regulated Channels

    Klausen, Thomas Kjær

    - serves a multitude of functions in the mammalian cell, regulating the membrane potential (Em), cell volume, protein activity and the driving force for facilitated transporters giving Cl- and Cl- channels a major potential of regulating cellular function. These functions include control of the cell cycle...... of volume perturbations evolution have developed system of channels and transporters to tightly control volume homeostasis. In the past decades evidence has been mounting, that the importance of these volume regulated channels and transporters are not restricted to the defense of cellular volume......, controlled cell death and cellular migration. Volume regulatory mechanisms has long been in focus for regulating cellular proliferation and my thesis work have been focusing on the role of Cl- channels in proliferation with specific emphasis on ICl, swell. Pharmacological blockage of the ubiquitously...

  10. Regulation of cholesterol homeostasis

    van der Wulp, Mariette Y. M.; Verkade, Henkjan J.; Groen, Albert K.


    Hypercholesterolemia is an important risk factor for cardiovascular disease. It is caused by a disturbed balance between cholesterol secretion into the blood versus uptake. The pathways involved are regulated via a complex interplay of enzymes, transport proteins, transcription factors and

  11. Regulation and controlled synchronization

    Huijberts, H.J.C.; Huijberts, H.J.C.; Nijmeijer, Henk; Willems, R.M.A.


    We investigate the problem of controlled synchronization as a regulator problem. In controlled synchronization one is given autonomous transmitter dynamics and controlled receiver dynamics. The question is to find a (output) feedback controller that achieves matching between transmitter and

  12. Palmitic acid interferes with energy metabolism balance by adversely switching the SIRT1-CD36-fatty acid pathway to the PKC zeta-GLUT4-glucose pathway in cardiomyoblasts.

    Chen, Yeh-Peng; Tsai, Chia-Wen; Shen, Chia-Yao; Day, Cecilia-Hsuan; Yeh, Yu-Lan; Chen, Ray-Jade; Ho, Tsung-Jung; Padma, V Vijaya; Kuo, Wei-Wen; Huang, Chih-Yang


    Metabolic regulation is inextricably linked with cardiac function. Fatty acid metabolism is a significant mechanism for creating energy for the heart. However, cardiomyocytes are able to switch the fatty acids or glucose, depending on different situations, such as ischemia or anoxia. Lipotoxicity in obesity causes impairments in energy metabolism and apoptosis in cardiomyocytes. We utilized the treatment of H9c2 cardiomyoblast cells palmitic acid (PA) as a model for hyperlipidemia to investigate the signaling mechanisms involved in these processes. Our results show PA induces time- and dose-dependent lipotoxicity in H9c2 cells. Moreover, PA enhances cluster of differentiation 36 (CD36) and reduces glucose transporter type 4 (GLUT4) pathway protein levels following a short period of treatment, but cells switch from CD36 back to the GLUT4 pathway after during long-term exposure to PA. As sirtuin 1 (SIRT1) and protein kinase Cζ (PKCζ) play important roles in CD36 and GLUT4 translocation, we used the SIRT1 activator resveratrol and si-PKCζ to identify the switches in metabolism. Although PA reduced CD36 and increased GLUT4 metabolic pathway proteins, when we pretreated cells with resveratrol to activate SIRT1 or transfected si-PKCζ, both were able to significantly increase CD36 metabolic pathway proteins and reduce GLUT4 pathway proteins. High-fat diets affect energy metabolism pathways in both normal and aging rats and involve switching the energy source from the CD36 pathway to GLUT4. In conclusion, PA and high-fat diets cause lipotoxicity in vivo and in vitro and adversely switch the energy source from the CD36 pathway to the GLUT4 pathway.

  13. Worldwide regulations for mycotoxins.

    van Egmond, Hans P


    Since the discovery of the aflatoxins in the 1960s, regulations have been established in many countries to protect the consumer from the harmful effects of mycotoxins that may contaminate foodstuffs. Various factors play a role in the decision-making process of setting limits for mycotoxins. These include scientific factors such as the availability of toxicological data, survey data, knowledge about the distribution of mycotoxins in commodities, and analytical methodology. Economical and political factors such as commercial interests and sufficiency of food supply have their impact as well. International enquiry's on existing mycotoxin legislation in foodstuffs and animal feedstuffs have been carried out several times in the 1980s and 1990s and details about tolerances, legal basis, responsible authorities, official protocols of analysis and sampling have been published. Recently a comprehensive update on worldwide regulations was published as FAO Food and Nutrition Paper 64. It appeared that at least 77 countries now have specific regulations for mycotoxins, 13 countries are known to have no specific regulations, whereas no data are available for about 50 countries, many of them in Africa. Over the years, a large diversity in tolerance levels for mycotoxins has remained. Some free trade zones (EU, MERCOSUR) are in the process of harmonizing the limits and regulations for mycotoxins in their respective member states, but it is not likely that worldwide harmonized limits for mycotoxins will soon be within reach.

  14. To regulate or not to regulate?

    Mason, G.; Wrixon, A. [IAEA, Vienna (Austria)


    Full text of publication follows: In Hamlet famous soliloquy to be or not to be, he wrestles with the perennial human problem of choosing the right course of action in difficult circumstances. In recent years, we have witnessed a cast of thousands playing out a long-running scene that seems to echo Hamlet dilemma on a rather more prosaic level. When is it necessary to apply regulations to the control of exposure to ionizing radiation and when is regulatory control not warranted? This seemingly straightforward question has brought out the philosopher, ethician, lawyer, pragmatist, orator in simple radiation protection folk and has led to passionate debate on numerous occasions. This paper attempts an answer based on a review of recent developments. For deciding when to apply regulatory controls, several concepts have evolved over time, including exemption of practices and sources, exclusion of exposures and clearance of materials. These have different origins, purposes and characteristics. Exemption and clearance have often been associated with triviality of risk, while exclusion has been related to un-amenability of control. For each concept, criteria have been developed to assist the regulator in reaching a decision, but there has much disputation over numerical values. This paper briefly reviews and analyses recent developments and attempts to clarify the problem from first principles. The conclusion is that the underlying issue in each case is to determine when regulatory controls become unwarranted: that is, when the societal resources expended in applying them and complying with them would be disproportionate to any benefit they might bring. This is a natural extension of the principle of optimization of protection to the regulatory control of protection, in the context of exposure to radiation at very low levels. It also reflects common expectations of good governance: wise management of finite societal resources and avoidance of unwarranted controls on

  15. Collaborative Tax Regulation

    Boll, Karen


    their customer bases decline to commercially non-viable levels. The analysis is framed by public governance literature and argues that the regulation is an example of collaborative or interactive governance, because the tax administrators do not regulate non-compliance directly, but activate external...... stakeholders, i.e. the consumers, in the regulatory craft. The study is based on a qualitative methodology and draws on a unique case of regulation in the cleaning sector. This sector is at high risk of tax evasion and human exploitation of vulnerable workers operating in the informal economy. The article has...... implications for how tax practitioners think about collaborative and interactive regulatory initiatives. While the tax administration in the study sees the approach as effective, the analysis shows that there are a number of caveats in relation to regularity, public listing, costs and revenue focus...

  16. Staff rules and regulations

    HR Department


    The 11th edition of the Staff Rules and Regulations, dated 1 January 2007, adopted by the Council and the Finance Committee in December 2006, is currently being distributed to departmental secretariats. The Staff Rules and Regulations, together with a summary of the main modifications made, will be available, as from next week, on the Human Resources Department's intranet site: The main changes made to the Staff Rules and Regulations stem from the five-yearly review of employment conditions of members of the personnel. The changes notably relate to: the categories of members of the personnel (e.g. removal of the local staff category); the careers structure and the merit recognition system; the non-residence, installation and re-installation allowances; the definition of family, family allowances and family-related leave; recognition of partnerships; education fees. The administrative circulars, some of which are being revised following the ...

  17. Collaborative Tax Regulation

    Boll, Karen


    This article shows a new form of regulation within a tax administration where tax administrators abate tax evasion by nudging and motivating consumers to only purchase services from tax compliant businesses. This indirectly closes or forces tax evading businesses to change their practices, because...... their customer bases decline to commercially non-viable levels. The analysis is framed by public governance literature and argues that the regulation is an example of collaborative or interactive governance, because the tax administrators do not regulate non-compliance directly, but activate external...... implications for how tax practitioners think about collaborative and interactive regulatory initiatives. While the tax administration in the study sees the approach as effective, the analysis shows that there are a number of caveats in relation to regularity, public listing, costs and revenue focus...

  18. Volume Regulated Channels

    Klausen, Thomas Kjær

    of volume perturbations evolution have developed system of channels and transporters to tightly control volume homeostasis. In the past decades evidence has been mounting, that the importance of these volume regulated channels and transporters are not restricted to the defense of cellular volume...... but are also essential for a number of physiological processes such as proliferation, controlled cell death, migration and endocrinology. The thesis have been focusing on two Channels, namely the swelling activated Cl- channel (ICl, swell) and the transient receptor potential Vanilloid (TRPV4) channel. I: Cl......- serves a multitude of functions in the mammalian cell, regulating the membrane potential (Em), cell volume, protein activity and the driving force for facilitated transporters giving Cl- and Cl- channels a major potential of regulating cellular function. These functions include control of the cell cycle...

  19. The Impact of Regulating Social Science Research with Biomedical Regulations

    Durosinmi, Brenda Braxton


    The Impact of Regulating Social Science Research with Biomedical Regulations Since 1974 Federal regulations have governed the use of human subjects in biomedical and social science research. The regulations are known as the Federal Policy for the Protection of Human Subjects, and often referred to as the "Common Rule" because 18 Federal…

  20. Other-Regulation in Collaborative Groups: Implications for Regulation Quality

    Rogat, Toni Kempler; Adams-Wiggins, Karlyn R.


    The current study examines variation in other-regulation, conceptualized as efforts by one student to regulate their group's work. This study extends research which has conceptualized other-regulation as temporarily guiding others' conceptual understanding and skill development by broadening the spectrum of other-regulation to include…

  1. The Impact of Regulating Social Science Research with Biomedical Regulations

    Durosinmi, Brenda Braxton


    The Impact of Regulating Social Science Research with Biomedical Regulations Since 1974 Federal regulations have governed the use of human subjects in biomedical and social science research. The regulations are known as the Federal Policy for the Protection of Human Subjects, and often referred to as the "Common Rule" because 18 Federal…

  2. Nuclear regulation and safety

    Hendrie, J.M.


    Nuclear regulation and safety are discussed from the standpoint of a hypothetical country that is in the process of introducing a nuclear power industry and setting up a regulatory system. The national policy is assumed to be in favor of nuclear power. The regulators will have responsibility for economic, reliable electric production as well as for safety. Reactor safety is divided into three parts: shut it down, keep it covered, take out the afterheat. Emergency plans also have to be provided. Ways of keeping the core covered with water are discussed. (DLC)

  3. Cyberplagiarism in University Regulations

    Santiago Cavanillas


    Full Text Available The article examines the legal framework for plagiarism, and its twofold nature of illicit appropriation (from the author of the plagiarized work and fraud (with regard to the target audience of the plagiarism. Based on these premises, academic cyberplagiarism is analysed as a form of plagiarism carried out using electronic tools in the university setting. The question of responsibility (who can regulate the legal consequences of plagiarism? before and after the Ley Orgánica de Universidades (organic law on universities, LOU is studied, as is the disciplinary handling of cyberplagiarism with the limited regulations currently in place at universities.

  4. Proof of Riemann Hypothesis about Non-trivial Zeros of the Zeta Function also Let the Old Methods Produce New Vitality & Exploration%枯树生花于“黎曼猜想之ζ零点分布”及探究



    作者非常自信自己完美地证明了仅剩的最后一个黎曼猜想[1]——ζ函数的零点分布假设。他的这种自信既来自于公理集合论中“任意无穷集合,它们的势都相等[2]”的这个经典定理,也来自于黎曼ζ函数所含有的一个重要性质,更来自于他的“双定理论”,还来自于他坚信自己曾经绝妙地证明了大众化的百年难题——哥德巴赫猜想。%The author is very confident that he perfectly proved Riemann hypothesis of non-trivial zeros of ζ. His confidence comes from the classical theorem in axiomatic set theory—"Any two different infinite sets always have the same cardinality", also from an important property of the Riemann zeta function, also from his "the theory of double fixed nature", and also from his firm belief that he had proved the problem that everyone under-stands but still has not been solved for hundreds of years, that is, the Goldbach's Conjecture.

  5. Design of dual pressure regulator

    Kim, Dong Soo; Kim, Kang Dae; Kim, Myoung Sub [Korea Institute of Machinery and Materials, Daejeon (Korea, Republic of)


    In this paper, we designed sandwich type pressure regulator for air pressure control system. As a result of research, we obtained several important conclusions. First, we decided theory of poppet valve and relief valve which are used in sandwich type pressure regulator, and then designed prototype of pressure regulator. Second, we organized circuit diagram of dual pressure regulator of air pressure control system.

  6. Markets, religion, regulation

    Fischer, Johan


    of regulation, certification and standardization on a global scale. Building on research on global kosher (a Hebrew term meaning “fit” or “proper”), halal (an Arabic word that literally means “permissible” or “lawful”) and Hindu vegetarianism this paper argues that these economies or markets to a large extent...

  7. Vehicle recycling regulations

    Smink, Carla


    The number of end-of-life vehicles (ELVs) in the EU is increasing continously. Around 75 percent of an ELV are recyclable metals. The forecast growth in the number of ELVs calls for regulation that aims to minimise the environmental impact of a car. Using Denmark as an example, this article...

  8. Situated bio-regulation

    Prainsack, Barbara; Wahlberg, Ayo


    Several years ago, both authors engaged in research into bioscience and biomedical regulation in Asian countries. One of us (BP) explored why the regulatory and discursive embedding of human embryonic stem cell in Israel was much more permissive than elsewhere. The other author (AW) sought to und...

  9. Regulated Gene Therapy.

    Breger, Ludivine; Wettergren, Erika Elgstrand; Quintino, Luis; Lundberg, Cecilia


    Gene therapy represents a promising approach for the treatment of monogenic and multifactorial neurological disorders. It can be used to replace a missing gene and mutated gene or downregulate a causal gene. Despite the versatility of gene therapy, one of the main limitations lies in the irreversibility of the process: once delivered to target cells, the gene of interest is constitutively expressed and cannot be removed. Therefore, efficient, safe and long-term gene modification requires a system allowing fine control of transgene expression.Different systems have been developed over the past decades to regulate transgene expression after in vivo delivery, either at transcriptional or post-translational levels. The purpose of this chapter is to give an overview on current regulatory system used in the context of gene therapy for neurological disorders. Systems using external regulation of transgenes using antibiotics are commonly used to control either gene expression using tetracycline-controlled transcription or protein levels using destabilizing domain technology. Alternatively, specific promoters of genes that are regulated by disease mechanisms, increasing expression as the disease progresses or decreasing expression as disease regresses, are also examined. Overall, this chapter discusses advantages and drawbacks of current molecular methods for regulated gene therapy in the central nervous system.

  10. Federal Gasoline Regulations

    The Clean Air Act requires EPA to regulate fuels and fuel additives for use in mobile sources if such fuel, fuel additive or any emission products causes or contributes to air or water pollution that may endanger the public health or welfare.

  11. Situated bio-regulation

    Prainsack, Barbara; Wahlberg, Ayo


    Several years ago, both authors engaged in research into bioscience and biomedical regulation in Asian countries. One of us (BP) explored why the regulatory and discursive embedding of human embryonic stem cell in Israel was much more permissive than elsewhere. The other author (AW) sought to und...

  12. Legislation and regulation



    This document presents the fulfilling of the Brazilian obligations under the Convention on Nuclear Safety. The Chapter 3 of the document contains some details about the Brazilian legislation and regulation, the legislative and regulatory framework, regulatory body and responsibility of the license holder.

  13. Regulation of cholesterol homeostasis

    van der Wulp, Mariette Y. M.; Verkade, Henkjan J.; Groen, Albert K.


    Hypercholesterolemia is an important risk factor for cardiovascular disease. It is caused by a disturbed balance between cholesterol secretion into the blood versus uptake. The pathways involved are regulated via a complex interplay of enzymes, transport proteins, transcription factors and non-codin

  14. Regulating groundwater use

    Hoogesteger van Dijk, Jaime; Wester, Flip


    Around the world it has proven very difficult to develop policies and interventions that ensure socio-environmentally sustainable groundwater use and exploitation. In the state of Guanajuato, Central Mexico, both the national government and the decentralized state government have pursued to regulate

  15. Regulating the private security industry

    Percy, Sarah


    The under-regulation of the private security industry has increasingly become a topic of media and academic interest. This Adelphi Paper enters the debate by explaining why the industry requires further regulation, and what is wrong with the current system. It begins by briefly defining the industry and explaining the need for more effective regulation, before analysing three types of regulation: domestic, international and informal (including self-regulation).

  16. Postsynaptic localization of PSD-95 is regulated by all three pathways downstream of TrkB signaling.

    Yoshii, Akira; Constantine-Paton, Martha


    Brain-derived neurotrophic factor (BDNF) and its receptor TrkB regulate synaptic plasticity. TrkB triggers three downstream signaling pathways; Phosphatidylinositol 3-kinase (PI3K), Phospholipase Cγ (PLCγ) and Mitogen activated protein kinases/Extracellular signal-regulated kinases (MAPK/ERK). We previously showed two distinct mechanisms whereby BDNF-TrkB pathway controls trafficking of PSD-95, which is the major scaffold at excitatory synapses and is critical for synapse maturation. BDNF activates the PI3K-Akt pathway and regulates synaptic delivery of PSD-95 via vesicular transport (Yoshii and Constantine-Paton, 2007). BDNF-TrkB signaling also triggers PSD-95 palmitoylation and its transport to synapses through the phosphorylation of the palmitoylation enzyme ZDHHC8 by a protein kinase C (PKC; Yoshii etal., 2011). The second study used PKC inhibitors chelerythrine as well as a synthetic zeta inhibitory peptide (ZIP) which was originally designed to block the brain-specific PKC isoform protein kinase Mϖ (PKMϖ). However, recent studies raise concerns about specificity of ZIP. Here, we assessed the contribution of TrkB and its three downstream pathways to the synaptic distribution of endogenous PSD-95 in cultured neurons using chemical and genetic interventions. We confirmed that TrkB, PLC, and PI3K were critical for the postsynaptic distribution of PSD-95. Furthermore, suppression of MAPK/ERK also disrupted PSD-95 expression. Next, we examined the contribution of PKC. While both chelerythrine and ZIP suppressed the postsynaptic localization of PSD-95, RNA interference for PKMϖ did not have a significant effect. This result suggests that the ZIP peptide, widely used as the "specific" PKMϖ antagonist by many investigators may block a PKC variant other than PKMϖ such as PKCλ/ι. Our results indicate that TrkB regulates postsynaptic localization of PSD-95 through all three downstream pathways, but also recommend further work to identify other PKC variants that

  17. Dimensionally regulated pentagon integrals

    Bern, Z; Kosower, D A


    We present methods for evaluating the Feynman parameter integrals associated with the pentagon diagram in 4-2 epsilon dimensions, along with explicit results for the integrals with all masses vanishing or with one non-vanishing external mass. The scalar pentagon integral can be expressed as a linear combination of box integrals, up to O(epsilon) corrections, a result which is the dimensionally-regulated version of a D=4 result of Melrose, and of van Neerven and Vermaseren. We obtain and solve differential equations for various dimensionally-regulated box integrals with massless internal lines, which appear in one-loop n-point calculations in QCD. We give a procedure for constructing the tensor pentagon integrals needed in gauge theory, again through O(epsilon^0).

  18. Probiotics and Appetite Regulation

    Bjerg, Anne Toksvig

    resistance and blood lipid profile among others. Probiotics which are health promoting bacteria can potentially be used to affect the GM and thereby change metabolic outcomes of the host. Animal studies have shown associations between intake of probiotics and appetite regulation, but currently no human...... studies have investigated this effect. Supplementation with different probiotic strains have been shown to have an effect on blood lipid profiles in both animals and humans and the mechanisms behind have been studied in vitro and in rodents. The aim of the present thesis was to examine in an ex vivo...... intestine, in an animal study and in two human studies the effect of the probiotic bacteria Lactobacillus paracasei subsp. paracasei L. casei W8 (W8) on appetite regulation, blood lipids and blood fatty acids. In addition, it was investigated if W8 had an effect on the fecal microbiota of the human...

  19. Regulation of Meiotic Recombination

    Gregory p. Copenhaver


    Meiotic recombination results in the heritable rearrangement of DNA, primarily through reciprocal exchange between homologous chromosome or gene conversion. In plants these events are critical for ensuring proper chromosome segregation, facilitating DNA repair and providing a basis for genetic diversity. Understanding this fundamental biological mechanism will directly facilitate trait mapping, conventional plant breeding, and development of genetic engineering techniques that will help support the responsible production and conversion of renewable resources for fuels, chemicals, and the conservation of energy (1-3). Substantial progress has been made in understanding the basal recombination machinery, much of which is conserved in organisms as diverse as yeast, plants and mammals (4, 5). Significantly less is known about the factors that regulate how often and where that basal machinery acts on higher eukaryotic chromosomes. One important mechanism for regulating the frequency and distribution of meiotic recombination is crossover interference - or the ability of one recombination event to influence nearby events. The MUS81 gene is thought to play an important role in regulating the influence of interference on crossing over. The immediate goals of this project are to use reverse genetics to identify mutants in two putative MUS81 homologs in the model plant Arabidopsis thaliana, characterize those mutants and initiate a novel forward genetic screen for additional regulators of meiotic recombination. The long-term goal of the project is to understand how meiotic recombination is regulated in higher eukaryotes with an emphasis on the molecular basis of crossover interference. The ability to monitor recombination in all four meiotic products (tetrad analysis) has been a powerful tool in the arsenal of yeast geneticists. Previously, the qrt mutant of Arabidopsis, which causes the four pollen products of male meiosis to remain attached, was developed as a facile system

  20. CNS regulation of appetite.

    Harrold, Joanne A; Dovey, Terry M; Blundell, John E; Halford, Jason C G


    This article reviews the regulation of appetite from a biopsychological perspective. It considers psychological experiences and peripheral nutritional systems (both episodic and tonic) and addresses their relationship with the CNS networks that process and integrate their input. Whilst such regulatory aspects of obesity focus on homeostatic control mechanisms, in the modern environment hedonic aspects of appetite are also critical. Enhanced knowledge of the complexity of appetite regulation and the mechanisms that sustain obesity indicate the challenge presented by management of the obesity epidemic. Nonetheless, effective control of appetite expression remains a critical therapeutic target for weight management. Currently, strategies which utilise a combination of agents to target both homeostatic and hedonic control mechanisms represent the most promising approaches. This article is part of a Special Issue entitled 'Central Control of Food Intake'. Copyright © 2012 Elsevier Ltd. All rights reserved.

  1. Hormonal Regulators of Appetite

    Austin Juliana; Marks Daniel


    Obesity is a significant cause of morbidity and mortality worldwide. There has been a significant worsening of the obesity epidemic mainly due to alterations in dietary intake and energy expenditure. Alternatively, cachexia, or pathologic weight loss, is a significant problem for individuals with chronic disease. Despite their obvious differences, both processes involve hormones that regulate appetite. These hormones act on specific centers in the brain that affect the sensations of hunger a...

  2. The regulation of hunting

    Abildtrup, Jens; Jensen, Frank


    This paper examines a tax/subsidy on hunters based on game population. The tax/subsidy is the difference between actual and optimal population multiplied by an individual, variable tax rate. The tax rate is, among other things, based on the difference between the marginal value of the game...... population to the hunter and the regulator and differences in user costs of the population. The paper shows that the population tax/subsidy secures a first-best optimum....

  3. Computing Borel's Regulator II

    Choo, Zacky; Sánchez-García, Rubén J; Snaith, Victor P


    In our earlier article we described a power series formula for the Borel regulator evaluated on the odd-dimensional homology of the general linear group of a number field and, concentrating on dimension three for simplicity, described a computer algorithm which calculates the value to any chosen degree of accuracy. In this sequel we give an algorithm for the construction of the input homology classes and describe the results of one cyclotomic field computation.

  4. [Sleep: regulation and phenomenology].

    Vecchierini, M-F


    This article describes the two-process model of sleep regulation. The 24-hour sleep-wake cycle is regulated by a homeostatic process and an endogenous, 2 oscillators, circadian process, under the influence of external synchronisers. These two processes are partially independent but influence each other, as shown in the two-sleep-process auto-regulation model. A reciprocal inhibition model of two interconnected neuronal groups, "SP on" and "SP off", explains the regular recurrence of paradoxical sleep. Sleep studies have primarily depended on observation of the subject and have determined the optimal conditions for sleep (position, external conditions, sleep duration and need) and have studied the consequences of sleep deprivation or modifications of sleep schedules. Then, electrophysiological recordings permitted the classification of sleep stages according to the observed EEG patterns. The course of a night's sleep is reported on a "hypnogram". The adult subject falls asleep in non-REM sleep (N1), then sleep deepens progressively to stages N2 and N3 with the appearance of spindles and slow waves (N2). Slow waves become more numerous in stage N3. Every 90minutes REM sleep recurs, with muscle atonia and rapid eye movements. These adult sleep patterns develop progressively during the 2 first years of life as total sleep duration decreases, with the reduction of diurnal sleep and of REM sleep. Around 2 to 4 months, spindles and K complexes appear on the EEG, with the differentiation of light and deep sleep with, however, a predominance of slow wave sleep.

  5. Staff rules and regulations

    HR Department


    The 11th edition of the Staff Rules and Regulations, dated 1 January 2007, adopted by the Council and the Finance Committee in December 2006, is currently being distributed to departmental secretariats. The Staff Rules and Regulations, together with a summary of the main modifications made, will be available, as from next week, on the Human Resources Department's intranet site: The main changes made to the Staff Rules and Regulations stem from the five-yearly review of employment conditions of members of the personnel. The changes notably relate to: the categories of members of the personnel (e.g. removal of the local staff category); the careers structure and the merit recognition system; the non-residence, installation and re-installation allowances; the definition of family, family allowances and family-related leave; recognition of partnerships; education fees. The administrative circulars, some of which are being revised following the m...

  6. Improving CS regulations.

    Nesse, R.J.; Scheer, R.M.; Marasco, A.L.; Furey, R.


    President Carter issued Executive Order 12044 (3/28/78) that required all Federal agencies to distinguish between significant and insignificant regulations, and to determine whether a regulation will result in major impacts. This study gathered information on the impact of the order and the guidelines on the Office of Conservation and Solar Energy (CS) regulatory practices, investigated problems encountered by the CS staff when implementing the order and guidelines, and recommended solutions to resolve these problems. Major tasks accomplished and discussed are: (1) legislation, Executive Orders, and DOE Memoranda concerning Federal administrative procedures relevant to the development and analysis of regulations within CS reviewed; (2) relevant DOE Orders and Memoranda analyzed and key DOE and CS staff interviewed in order to accurately describe the current CS regulatory process; (3) DOE staff from the Office of the General Counsel, the Office of Policy and Evaluation, the Office of the Environment, and the Office of the Secretary interviewed to explore issues and problems encountered with current CS regulatory practices; (4) the regulatory processes at five other Federal agencies reviewed in order to see how other agencies have approached the regulatory process, dealt with specific regulatory problems, and responded to the Executive Order; and (5) based on the results of the preceding four tasks, recommendations for potential solutions to the CS regulatory problems developed. (MCW)

  7. Polysaccharide zeta-potentials and protein-affinity.

    Comert, Fatih; Azarikia, Fatemeh; Dubin, Paul L


    The ζ-potential, a parameter typically obtained by model-dependent transformation of the measured electrophoretic mobility, is frequently used to understand polysaccharide-protein complexation. We tested the hypothesis that two anionic polysaccharides with identical ζ-potentials would show equal binding affinity to the protein β-lactoglobulin (BLG). We selected two polysaccharide polyelectrolytes (PE) with very different structures: hyaluronic acid (HA) and tragacanthin (TG). Highly precise (±0.1%) turbidimetric titrations were performed to determine critical pH values of complex formation; and PE ζ-potentials were measured for different ionic strengths I at those critical pH values. While phase boundaries (pHcvs. I) showed that HA binds to BLG more strongly (e.g. at a lower pH, for fixed I), comparisons made at fixed ζ-potential indicated that TG binds more strongly. The source of this contradiction is the effect of the bulky side chains of TG on its friction coefficient which diminishes its mobility and hence the resultant ζ-potential; while having a distinctly separate effect on the interaction between BLG and the carboxylated backbone of TG. Thus, unless the locus of the bound protein coincides with the shear plane, the ζ-potential does not directly contribute to the electrostatic PE-protein interaction.

  8. Progress in nanoparticles characterization:Sizing and zeta potential measurement

    Renliang Xu


    Characterization of various nanoparticles is on the center stage in nanotechnology development. The subjects for nanoparticles characterization are focused on particle size and particle surface charge determinations. This article summarizes the latest development in particle size analysis using dynamic light scattering and surface charge determination using electrophoretic light scattering for nano- or even sub-nanoparticles in concentrated suspensions.

  9. Reload Purified Melittin and Lactoferrin on Perfluorooctyl Bromide Nanoparticles (PFOB-Nps and Examine the Distribution of Particle Size, Zeta Potential and Confirmation of Their Accession on the Nanoparticles Via Tryptophan Fluorescence and Circular Dichroism (CD and its Anti-Cancer Effects on Human Breast Cancer Cell Line MCF7

    Hadis Rahmani Incheh Keykanlu


    Full Text Available Acording to the prevalence of cancer in today's societies, it is too important to find new drugs for that treatment. Cationic antimicrobial peptides that are able to eliminate a wide range of bacteria, fungi, parasites, viruses and unicellular, are natural anti-cancer agents. In the present study, the effect of cationic peptides of bee venom Melittin (ML and Lactoferrin (LF of camel milk which was loaded on the Perfluorooctyl Bromide (PFOB nanoparticles, were examined on MCF7 cells. Nanoparticles using oil in water emulsion was prepared by ultra-thoracic and ultrasonic. Then ML and LF were separately added to the nanoparticles then incorporation of them were confirmed by tryptophan fluorescence and circular dichroism (CD spectra. The mean diameter of the nanoparticles did not change after addition of ML and LF (~100 nm, but as expected, the zeta potential of the nanoparticles with increasing of ML and LF concentrations to the preformed nanoparticles increased from -18.43(without ML and LF to +21.61(LF 110.66µg/ml and +20.93(ML 0.1µg/ml and +23.75(LF 110.66µg/ml and ML 0.1µg/ml. By using of scanning electron microscopy (SEM, the morphology and structure of nanoparticles was investigated, homogeneity and uniformity during processing and loading of ML and LF was observed. Effectiveness of nanoparticles loaded on cancer cells was performed with MTT test. The results showed that ML and LF had a positive effect on cancer cell death. Percent of cell death was higher when ML and LF was loaded on nanoparticles. With increase of ML and LF concentration loading on nanoparticles, increases cancer cell death. Minitab software was used to analyze some data.

  10. Environmental regulations and business decisions

    Gray, Wayne B.


    Environmental regulations raise production costs at regulated firms, though in most cases the costs are only a small fraction of a firm’s total costs. Productivity tends to fall, and firms may shift new investment and production to locations with less stringent regulation. However, environmental regulations have had enormous benefits in terms of lives saved and illnesses averted, especially through reductions in airborne particulates. The potential health gains may be even greater in developi...

  11. [Regulation of terpene metabolism

    Croteau, R.


    This report describes accomplishments over the past year on understanding of terpene synthesis in mint plants and sage. Specifically reported are the fractionation of 4-S-limonene synthetase, the enzyme responsible for the first committed step to monoterpene synthesis, along with isolation of the corresponding RNA and DNA cloning of its gene; the localization of the enzyme within the oil glands, regulation of transcription and translation of the synthetase, the pathway to camphor biosynthesis,a nd studies on the early stages and branch points of the isoprenoid pathway.




    Literal translation and liberal translation are two important methods and both play key roles in translation.However,some textbooks say that most translations are literal translations while others maintain most are liberal ones,besides,some others suggest a combination of the two.This paper focuses on the facts that regulate liberal translation.Because of the differences in culture,society,history,geography,and so on,there exists a great difference between Chinese language and English language,which does naturally lead to the liberal translation.

  13. The regulation of hunting

    Abildtrup, Jens; Jensen, Frank

    of the individual harvest. However, information about the individual harvest may be costly to obtain. Thus, we may have to look for alternatives to the existing system. This paper proposes a population tax/subsidy as an alternative which is the difference between the actual and optimal population multiplied...... by an individual, variable tax rate. The variable tax rate is, among other things, based on the difference in marginal value of the population between the hunter and the regulator. The paper shows that the population tax/subsidy secures a first-best optimum. Thus, the population tax is a good alternative...

  14. Wiring regulations in brief

    Tricker, Ray


    Tired of trawling through the Wiring Regs?Perplexed by Part P?Confused by cables, conductors and circuits?Then look no further! This handy guide provides an on-the-job reference source for Electricians, Designers, Service Engineers, Inspectors, Builders, Students, DIY enthusiastsTopic-based chapters link areas of working practice - such as cables, installations, testing and inspection, special locations - with the specifics of the Regulations themselves. This allows quick and easy identification of the official requirements relating to the situati

  15. The Regulation of Street Foods

    Forkour, John Boulard; Samuelsen, Helle; Yeboah, Eric Henry


    the challenges and negotiating strategies of regulators of street-vended foods in Ghana and analyses the implication for their relationship with street food vendors. The paper reveals that regulators operate in a context of limited resources, leading to a general feeling of neglect. In coping, regulators adopt...

  16. [Regulation of terpene metabolism

    Croteau, R.


    During the last grant period, we have completed studies on the key pathways of monoterpene biosynthesis and catabolism in sage and peppermint, and have, by several lines of evidence, deciphered the rate-limiting step of each pathway. We have at least partially purified and characterized the relevant enzymes of each pathway. We have made a strong case, based on analytical, in vivo, and in vitro studies, that terpene accumulation depends upon the balance between biosynthesis and catabolism, and provided supporting evidence that these processes are developmentally-regulated and very closely associated with senescence of the oil glands. Oil gland ontogeny has been characterized at the ultrastructural level. We have exploited foliar-applied bioregulators to delay gland senescence, and have developed tissue explant and cell culture systems to study several elusive aspects of catabolism. We have isolated pure gland cell clusters and localized monoterpene biosynthesis and catabolism within these structures, and have used these preparations as starting materials for the purification to homogeneity of target regulatory'' enzymes. We have thus developed the necessary background knowledge, based on a firm understanding of enzymology, as well as the necessary experimental tools for studying the regulation of monoterpene metabolism at the molecular level. Furthermore, we are now in a position to extend our systematic approach to other terpenoid classes (C[sub 15]-C[sub 30]) produced by oil glands.

  17. Buck/boost regulator

    Paulkovich, J.; Rodriguez, G. E. (Inventor)


    A voltage regulated DC to DC converter uses an inductor and a capacitor as storage elements. The inductor is composed of two windings having a common junction. A transformer with a center tap connected to the common junction of the two windings is connected at either end of its winding to ground through controlled switches. One winding of the inductor and either end of the transformer winding are connected by power diodes to the capacitor which supplies the output voltage to a load. The other winding of the inductor is connected to a fourth power diode as a clamping diode. Input voltage is supplied to the inductor through a third controlled switch. A pulse width modulator connected to the output of the converter alternately closes and opens the switches connected to either end of the transformer winding and also closes the switch supplying input voltage to the inductor each time either of the switches connected to the ends of the transformer winding are closed. The duty cycle of the closing and opening of the several switches is adjusted by the pulse modulator to regulate the output voltage.

  18. Regulations and Procedures Manual

    Young, Lydia J. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)


    The purpose of the Regulations and Procedures Manual (RPM) is to provide LBNL personnel with a reference to University and Lawrence Berkeley National Laboratory (LBNL or Laboratory) policies and regulations by outlining normal practices and answering most policy questions that arise in the day-to-day operations of Laboratory organizations. Much of the information in this manual has been condensed from detail provided in LBNL procedure manuals, Department of Energy (DOE) directives, and Contract DE-AC02-05CH11231. This manual is not intended, however, to replace any of those documents. RPM sections on personnel apply only to employees who are not represented by unions. Personnel policies pertaining to employees represented by unions may be found in their labor agreements. Questions concerning policy interpretation should be directed to the LBNL organization responsible for the particular policy. A link to the Managers Responsible for RPM Sections is available on the RPM home page. If it is not clear which organization is responsible for a policy, please contact Requirements Manager Lydia Young or the RPM Editor.

  19. Circadian rhythms regulate amelogenesis.

    Zheng, Li; Seon, Yoon Ji; Mourão, Marcio A; Schnell, Santiago; Kim, Doohak; Harada, Hidemitsu; Papagerakis, Silvana; Papagerakis, Petros


    Ameloblasts, the cells responsible for making enamel, modify their morphological features in response to specialized functions necessary for synchronized ameloblast differentiation and enamel formation. Secretory and maturation ameloblasts are characterized by the expression of stage-specific genes which follows strictly controlled repetitive patterns. Circadian rhythms are recognized as key regulators of the development and diseases of many tissues including bone. Our aim was to gain novel insights on the role of clock genes in enamel formation and to explore the potential links between circadian rhythms and amelogenesis. Our data shows definitive evidence that the main clock genes (Bmal1, Clock, Per1 and Per2) oscillate in ameloblasts at regular circadian (24 h) intervals both at RNA and protein levels. This study also reveals that the two markers of ameloblast differentiation i.e. amelogenin (Amelx; a marker of secretory stage ameloblasts) and kallikrein-related peptidase 4 (Klk4, a marker of maturation stage ameloblasts) are downstream targets of clock genes. Both, Amelx and Klk4 show 24h oscillatory expression patterns and their expression levels are up-regulated after Bmal1 over-expression in HAT-7 ameloblast cells. Taken together, these data suggest that both the secretory and the maturation stages of amelogenesis might be under circadian control. Changes in clock gene expression patterns might result in significant alterations of enamel apposition and mineralization.

  20. The Quality of Regulation

    Emil BĂLAN


    Full Text Available Good governance also involves the affirmation and practice of some principles that allow the structures of the public space to administrate the general interest in the respect of the democracy and of the state of law pre-requisites, as well as the ones of a good administration: trust and predictability, openness and transparency, responsibility, efficiency and efficacy. The assurance of such desideratum imposes that the rules invested with the force of law, applicable to the juridical rapports to be clear, not equivoques, predictable, to allow both the protection of public interest and the respect of the citizens’ dignity and interests. The evaluation of the quality of regulation represents a necessary process of the appreciation of the impact that juridical norms are intended to produce and measures in which the outcomes of the implementation correspond to the ones established during the stage of formulating the public policy. The study tries to identify ways of evaluating the quality of regulation, valid in a social and political space governed by democratic rules and principles.

  1. Regulations and Procedures Manual

    Young, Lydia [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)


    The purpose of the Regulations and Procedures Manual (RPM) is to provide Laboratory personnel with a reference to University and Lawrence Berkeley National Laboratory policies and regulations by outlining the normal practices and answering most policy questions that arise in the day-to-day operations of Laboratory departments. Much of the information in this manual has been condensed from detail provided in Laboratory procedure manuals, Department of Energy (DOE) directives, and Contract DE-AC02-05CH11231. This manual is not intended, however, to replace any of those documents. The sections on personnel apply only to employees who are not represented by unions. Personnel policies pertaining to employees represented by unions may be found in their labor agreements. Questions concerning policy interpretation should be directed to the department responsible for the particular policy. A link to the Managers Responsible for RPM Sections is available on the RPM home page. If it is not clear which department should be called, please contact the Associate Laboratory Director of Operations.

  2. Regulation as delegation

    Oren Bar-Gill


    Full Text Available Objective to consider the conception of reverse delegation when the government acts a principal and an individual ndash an agent from the point of view of behavioral PrincipalAgent Theory. Methods statistical method sociological polling. Results In diverse areas ndash from retirement savings to consumer credit to prescription drug use to fuel economy and energy efficiency rules to tobacco consumption to food and beverage consumption ndash government makes decisions for us or endeavors to help us make better decisions thus serving as our agent. From the point of view of PrincipalAgent Theory and behavioral PrincipalAgent Theory a great deal of modern regulation can be helpfully evaluated as a hypothetical delegation. Shifting from personal decisions to public goods problems the authors view the idea of reverse delegation with the government as principal and the individuals as agents. They show that the essence of delegation changes depending on the context. The article describes conditions under which various approaches will make sense. Scientific novelty the paper is devoted to the foreign experience of regulation through delegation by the example of a country with developed market economy the USA. It shows the prospects of such approach in solving both the public and the private tasks. Application of PrincipalAgent Theory and behavioral PrincipalAgent Theory is viewed to distinguish between such types of hypothetical delegation as information default rules incentives precommitments mandates and prohibitions. The article considers the benefits and costs of delegation and circumstances in which one or another approach makes sense. Practical significance PrincipalAgent Theory is widely used in economics and political science and can serve as a convenient tool to consider the optimal scale and essence of the assistance rendered to us by the government as our agent. The paper is of interest for the Russian legal science as the institution of

  3. Hormonal Regulators of Appetite

    Juliana Austin


    Full Text Available Obesity is a significant cause of morbidity and mortality worldwide. There has been a significant worsening of the obesity epidemic mainly due to alterations in dietary intake and energy expenditure. Alternatively, cachexia, or pathologic weight loss, is a significant problem for individuals with chronic disease. Despite their obvious differences, both processes involve hormones that regulate appetite. These hormones act on specific centers in the brain that affect the sensations of hunger and satiety. Mutations in these hormones or their receptors can cause substantial pathology leading to obesity or anorexia. Identification of individuals with specific genetic mutations may ultimately lead to more appropriate therapies targeted at the underlying disease process. Thus far, these hormones have mainly been studied in adults and animal models. This article is aimed at reviewing the hormones involved in hunger and satiety, with a focus on pediatrics.

  4. Hormonal Regulators of Appetite

    Austin Juliana


    Full Text Available Obesity is a significant cause of morbidity and mortality worldwide. There has been a significant worsening of the obesity epidemic mainly due to alterations in dietary intake and energy expenditure. Alternatively, cachexia, or pathologic weight loss, is a significant problem for individuals with chronic disease. Despite their obvious differences, both processes involve hormones that regulate appetite. These hormones act on specific centers in the brain that affect the sensations of hunger and satiety. Mutations in these hormones or their receptors can cause substantial pathology leading to obesity or anorexia. Identification of individuals with specific genetic mutations may ultimately lead to more appropriate therapies targeted at the underlying disease process. Thus far, these hormones have mainly been studied in adults and animal models. This article is aimed at reviewing the hormones involved in hunger and satiety, with a focus on pediatrics.

  5. Mitosis and its regulation

    Frías Vázquez Sara


    Full Text Available Cell division by mitosis is essential for the development of organisms and their reproduction; it is also neces- sary that each new cell is genetically identical to that from which it comes. In eukaryotes this is achieved by the presence of complex mechanisms that ensure the integrity of genomic material and their proper segregation during mitosis. The traditional view of mitosis has been divided into different stages that were characterized by morphological studies in dividing cells; advances in molecular biology have led beyond this characterization, so that we now know a range of participant molecules. This article will discuss the process of mitosis, both at the cellular and molecular level and a brief summary of the molecular players that regulate this process.

  6. Meat and Appetite Regulation

    Kehlet, Ursula Nana

    D thesis was to investigate the effects of fiber addition to meatballs and the effects of cooking methods of pork on appetite regulation. The PhD thesis is based on three human meal test studies and one analytical study related to the characteristics of fiber meat products. In paper I, the objective...... pork products are also characterized as high fat products containing more than 10 g fat per 100 g. In this context, the Danish meat industry puts a lot of effort into developing meat products with a healthier nutritional profile. Thus, it is relevant to provide scientific evidence of the satiating...... effects of new formulations of pork products. Different strategies can be applied to potentially enhance the satiating properties of pork. Processed meat products such as meatballs can serve as a matrix for the addition of fiber ingredients. Based on their high protein and fiber contents, high...

  7. Magnetostrictive Pressure Regulating System

    Richard, James A. (Inventor); Pickens, Herman L. (Inventor)


    A magnetostrictive pressure regulating system includes a magnetostrictive valve that incorporates a magnetostrictive actuator with at least one current-carrying coil disposed thereabout. A pressure force sensor, in fluid communication with the fluid exiting the valve, includes (i) a magnetostrictive material, (ii) a magnetic field generator in proximity to the magnetostrictive material for inducing a magnetic field in and surrounding the magnetostrictive material wherein lines of magnetic flux passing through the magnetostrictive material are defined, and (iii) a sensor positioned adjacent to the magnetostrictive material and in the magnetic field for measuring changes in at least one of flux angle and flux density when the magnetostrictive material experiences an applied force that is aligned with the lines of magnetic flux. The pressure of the fluid exiting the valve causes the applied force. A controller coupled to the sensor and to the current-carrying coil adjusts a current supplied to the current-carrying coil based on the changes so-measured.

  8. NCAM regulates cell motility

    Prag, Søren; Lepekhin, Eugene A; Kolkova, Kateryna


    Cell migration is required during development of the nervous system. The regulatory mechanisms for this process, however, are poorly elucidated. We show here that expression of or exposure to the neural cell adhesion molecule (NCAM) strongly affected the motile behaviour of glioma cells...... independently of homophilic NCAM interactions. Expression of the transmembrane 140 kDa isoform of NCAM (NCAM-140) caused a significant reduction in cellular motility, probably through interference with factors regulating cellular attachment, as NCAM-140-expressing cells exhibited a decreased attachment...... to a fibronectin substratum compared with NCAM-negative cells. Ectopic expression of the cytoplasmic part of NCAM-140 also inhibited cell motility, presumably via the non-receptor tyrosine kinase p59(fyn) with which NCAM-140 interacts. Furthermore, we showed that the extracellular part of NCAM acted as a paracrine...

  9. Regulation of cholesterol homeostasis.

    van der Wulp, Mariëtte Y M; Verkade, Henkjan J; Groen, Albert K


    Hypercholesterolemia is an important risk factor for cardiovascular disease. It is caused by a disturbed balance between cholesterol secretion into the blood versus uptake. The pathways involved are regulated via a complex interplay of enzymes, transport proteins, transcription factors and non-coding RNA's. The last two decades insight into underlying mechanisms has increased vastly but there are still a lot of unknowns, particularly regarding intracellular cholesterol transport. After decades of concentration on the liver, in recent years the intestine has come into focus as an important control point in cholesterol homeostasis. This review will discuss current knowledge of cholesterol physiology, with emphasis on cholesterol absorption, cholesterol synthesis and fecal excretion, and new (possible) therapeutic options for hypercholesterolemia.

  10. Regulation of Terpene Metabolism

    Rodney Croteau


    OAK-B135 Research over the last four years has progressed fairly closely along the lines initially proposed, with progress-driven expansion of Objectives 1, 2 and 3. Recent advances have developed from three research thrusts: 1. Random sequencing of an enriched peppermint oil gland cDNA library has given access to a large number of potential pathway and regulatory genes for test of function; 2. The availability of new DNA probes and antibodies has permitted investigation of developmental regulation and organization of terpenoid metabolism; and 3. The development of a transformation system for peppermint by colleagues at Purdue University has allowed direct transgenic testing of gene function and added a biotechnological component to the project. The current status of each of the original research objectives is outlined below.

  11. Higher regulators, algebraic

    Bloch, Spencer J


    This book is the long-awaited publication of the famous Irvine lectures. Delivered in 1978 at the University of California at Irvine, these lectures turned out to be an entry point to several intimately-connected new branches of arithmetic algebraic geometry, such as regulators and special values of L-functions of algebraic varieties, explicit formulas for them in terms of polylogarithms, the theory of algebraic cycles, and eventually the general theory of mixed motives which unifies and underlies all of the above (and much more). In the 20 years since, the importance of Bloch's lectures has not diminished. A lucky group of people working in the above areas had the good fortune to possess a copy of old typewritten notes of these lectures. Now everyone can have their own copy of this classic work.

  12. Probiotics and Appetite Regulation

    Bjerg, Anne Toksvig

    -armed parallel four weeks intervention study with W8 (1010 CFU) or placebo capsules was performed on young, normal to overweight participants. In the four weeks intervention study the effects of W8 on appetite, blood lipids, SCD1 activity and fecal microbiota were also investigated. Finally, associations between......Summary There is emerging focus on the gut microbiota’s (GM) effects on health. GM is suggested to be a contributing factor to the rapid development of obesity and its related diseases like type 2 diabetes and cardiovascular disease. The omposition of the GM has been associated with weight, insulin...... intestine, in an animal study and in two human studies the effect of the probiotic bacteria Lactobacillus paracasei subsp. paracasei L. casei W8 (W8) on appetite regulation, blood lipids and blood fatty acids. In addition, it was investigated if W8 had an effect on the fecal microbiota of the human...

  13. [Regulation of terpene metabolism

    Croteau, R.


    Terpenoid oils, resins, and waxes from plants are important renewable resources. The objective of this project is to understand the regulation of terpenoid metabolism using the monoterpenes (C[sub 10]) as a model. The pathways of monoterpene biosynthesis and catabolism have been established, and the relevant enzymes characterized. Developmental studies relating enzyme levels to terpene accumulation within the oil gland sites of synthesis, and work with bioregulators, indicate that monoterpene production is controlled by terpene cyclases, the enzymes catalyzing the first step of the monoterpene pathway. As the leaf oil glands mature, cyclase levels decline and monoterpene biosynthesis ceases. Yield then decreases as the monoterpenes undergo catabolism by a process involving conversion to a glycoside and transport from the leaf glands to the root. At this site, the terpenoid is oxidatively degraded to acetate that is recycled into other lipid metabolites. During the transition from terpene biosynthesis to catabolism, the oil glands undergo dramatic ultrastructural modification. Degradation of the producing cells results in mixing of previously compartmentized monoterpenes with the catabolic enzymes, ultimately leading to yield decline. This regulatory model is being applied to the formation of other terpenoid classes (C[sub 15] C[sub 20], C[sub 30], C[sub 40]) within the oil glands. Preliminary investigations on the formation of sesquiterpenes (C[sub 15]) suggest that the corresponding cyclases may play a lesser role in determining yield of these products, but that compartmentation effects are important. From these studies, a comprehensive scheme for the regulation of terpene metabolism is being constructed. Results from this project wail have important consequences for the yield and composition of terpenoid natural products that can be made available for industrial exploitation.

  14. Regulation of melanopsin expression.

    Hannibal, Jens


    Circadian rhythms in mammals are adjusted daily to the environmental day/night cycle by photic input via the retinohypothalamic tract (RHT). Retinal ganglion cells (RGCs) of the RHT constitute a separate light-detecting system in the mammalian retina used for irradiance detection and for transmission to the circadian system and other non-imaging forming processes in the brain. The RGCs of the RHT are intrinsically photosensitive due to the expression of melanopsin, an opsin-like photopigment. This notion is based on anatomical and functional data and on studies of mice lacking melanopsin. Furthermore, heterologous expression of melanopsin in non-neuronal mammalian cell lines was found sufficient to render these cells photosensitive. Even though solid evidence regarding the function of melanopsin exists, little is known about the regulation of melanopsin gene expression. Studies in albino Wistar rats showed that the expression of melanopsin is diurnal at both the mRNA and protein levels. The diurnal changes in melanopsin expression seem, however, to be overridden by prolonged exposure to light or darkness. Significant increase in melanopsin expression was observed from the first day in constant darkness and the expression continued to increase during prolonged exposure in constant darkness. Prolonged exposure to constant light, on the other hand, decreased melanopsin expression to an almost undetectable level after 5 days of constant light. The induction of melanopsin by darkness was even more pronounced if darkness was preceded by light suppression for 5 days. These observations show that dual mechanisms regulate melanopsin gene expression and that the intrinsic light-responsive RGCs in the albino Wistar rat adapt their expression of melanopsin to environmental light and darkness.

  15. Endocannabinoids in cerebrovascular regulation.

    Benyó, Zoltán; Ruisanchez, Éva; Leszl-Ishiguro, Miriam; Sándor, Péter; Pacher, Pál


    The cerebral blood flow is tightly regulated by myogenic, endothelial, metabolic, and neural mechanisms under physiological conditions, and a large body of recent evidence indicates that inflammatory pathways have a major influence on the cerebral blood perfusion in certain central nervous system disorders, like hemorrhagic and ischemic stroke, traumatic brain injury, and vascular dementia. All major cell types involved in cerebrovascular control pathways (i.e., smooth muscle, endothelium, neurons, astrocytes, pericytes, microglia, and leukocytes) are capable of synthesizing endocannabinoids and/or express some or several of their target proteins [i.e., the cannabinoid 1 and 2 (CB1 and CB2) receptors and the transient receptor potential vanilloid type 1 ion channel]. Therefore, the endocannabinoid system may importantly modulate the regulation of cerebral circulation under physiological and pathophysiological conditions in a very complex manner. Experimental data accumulated since the late 1990s indicate that the direct effect of cannabinoids on cerebral vessels is vasodilation mediated, at least in part, by CB1 receptors. Cannabinoid-induced cerebrovascular relaxation involves both a direct inhibition of smooth muscle contractility and a release of vasodilator mediator(s) from the endothelium. However, under stress conditions (e.g., in conscious restrained animals or during hypoxia and hypercapnia), cannabinoid receptor activation was shown to induce a reduction of the cerebral blood flow, probably via inhibition of the electrical and/or metabolic activity of neurons. Finally, in certain cerebrovascular pathologies (e.g., subarachnoid hemorrhage, as well as traumatic and ischemic brain injury), activation of CB2 (and probably yet unidentified non-CB1/non-CB2) receptors appear to improve the blood perfusion of the brain via attenuating vascular inflammation.

  16. Standard types of regulation loops; Chaines de regulation types

    Bertrand, M. [ENSAM, Centre d`Enseignement et de Recherche de Lille, 59 - Lille (France)


    The aim of this paper is to give help in the analysis of industrial regulation problems using different types of real installations. The increasing complexity of industrial systems requires the use of a decomposition-recomposition procedure using a scheme with different blocs. Examples are given to help the non-specialist users in the mastery of essential choices and in the distinction between operational and material separations. The examples concern: the heating loop of a central heating installation, the sensors and actuators of industrial systems (the temperature regulation of a tubular furnace, the electro-hydraulic positioning systems used in machine tools, forming, aeronautics etc.., the regulation of a mixing system for hot and cold fluids, and the regulation of a fluidizing system. The usual types of regulation loops are presented with the different steps of the resolution of a regulation problem. (J.S.) 7 refs.

  17. Branded prescription drug fee. Final regulations, temporary regulations, and removal of temporary regulations.


    This document contains final regulations that provide guidance on the annual fee imposed on covered entities engaged in the business of manufacturing or importing branded prescription drugs. This fee was enacted by section 9008 of the Patient Protection and Affordable Care Act, as amended by section 1404 of the Health Care and Education Reconciliation Act of 2010. This document also withdraws the Branded Prescription Drug Fee temporary regulations and contains new temporary regulations regarding the definition of controlled group that apply beginning on January 1, 2015. The final regulations and the new temporary regulations affect persons engaged in the business of manufacturing or importing certain branded prescription drugs. The text of the temporary regulations in this document also serves as the text of proposed regulations set forth in a notice of proposed rulemaking (REG-123286-14) on this subject in the Proposed Rules section in this issue of the Federal Register.

  18. Regulation of the power sector


    Regulation of the Power Sector is a unified, consistent and comprehensive treatment of the theories and practicalities of regulation in modern power-supply systems. The need for generation to occur at the time of use occasioned by the impracticality of large-scale electricity storage coupled with constant and often unpredictable changes in demand make electricity-supply systems large, dynamic and complex and their regulation a daunting task. Conceptually arranged in four parts, this book addresses both traditional regulatory frameworks and also liberalized and re-regulated environments. First, an introduction gives a full characterization of power supply including engineering, economic and regulatory viewpoints. The second part presents the fundamentals of regulation and the third looks at the regulation of particular components of the power sector in detail. Advanced topics and subjects still open or subject to dispute form the content of the fourth part. In a sector where regulatory design is the key driver...

  19. Nanomaterials: Regulation and Risk Assessment

    Hansen, Steffen Foss; Grieger, Khara Deanne; Baun, Anders


    The topics of regulation and risk assessment of nanomaterials have never been more relevant and controversial in Europe than they are at this point in time. In this entry, we present and discuss a number of major pieces of legislation relevant for the regulation of nanomaterials, including REACH...... Regulation. Chemical risk assessment provides a fundamental element in support of existing legislation. Risk assessment is normally said to consist of four elements, i.e., hazard identification, dose–response assessment, exposure assessment, and risk characterization. Each of these four elements hold......, the Water Framework Directive, pharmaceuticals regulation, and the Novel Foods Regulation. Current regulation of nanomaterials entail three overall challenges: 1) limitations in regard to terminology and definitions of key terms such as a “substance,” “novel food,” etc.; 2) safety assessment requirements...

  20. Sustainable regulation of construction.


    The seminar examined the role building codes and regulations can have in promoting a more sustainable approach to construction, particularly through their application to non-industrial building materials. A range of building materials such as straw, bamboo, rammed earth, adobe, and cob (a mixture of clay and chopped straw) were described and illustrated by slides to show their building potential. The current codes have a prime concern to protect the health and safety of people from the built environment. They have been developed almost exclusively for mainstream industrial materials and methods of construction, which makes them difficult to use with alternative, indigenous, or non-industrial building materials, even though those materials may be considered more sustainable. The argument was put forward that with only one-third of the world population living in modern industrial buildings today, it is not sustainable to re-house the remaining rapidly expanding population in high technology dwellings. Many of the low technology building materials and methods now used by the majority of people in the world need only incremental improvement to be equal or superior to many of their industrial replacements. Since these can be more sustainable methods of building, there needs to be an acceptance of the use of alternative materials, particularly in the developing parts of the world, where they are being rejected for less sustainable industrial methods. However, many codes make it difficult to use non-industrial materials; indeed, many of the industrial materials would not meet the demands that must be now met if they were now being introduced as new materials. Consequently, there is a need to develop codes to facilitate the use of a wider range of materials than in current use, and research is needed to assist this development. Sustainable regulation should take into account the full range of real impacts that materials and systems have in areas such as resource use and

  1. The Structure of Financial Regulation

    Bratu Renate Doina; Petria Nicolae


    During the current global financial crisis has called into question the role and objectives of the regulation of financial markets and its scope of action. Liberalization of financial services under the impact of globalization and strong innovative character of the financial system, architecture of regulation has generated a change. In this sense, this paper aims to present and evaluate the structure of financial system regulation in the current international context.

  2. Nitrate Reductase: Properties and Regulation


    Nitrate Reductase (NR) is a rating-limit and key enzyme of nitrate assimilation in plants ,so ,NR activity is important for growth,development and the dry matter accumulation of plants. The regulation of NR activity appears to be rather complex and many studies have been devoted to the description of regulation and properties,but in this paper we focus on the properties and regulation of NR in higher plants.

  3. Regulations on Open Government Information



    @@ On April 24, 2007 the State Council promulgated Regulations of the People's Republic of China on Open Government Information (referred to as Regulations below), which will become effective on May 1, 2008. As the first administrative rule of the central government of China that aims to safeguard the public's right to know, the Regulations are of great significance in China's democratization and its establishment of the rule of law.

  4. Personality and Emotion Regulation Strategies

    Esti Hayu Purnamaningsih


    Full Text Available The emotions has many important functions in our life such as in relation of interpersonal communication, and health. In interpersonal communicative function aimed to signal to other information about internal state. Emotions manifests in specific cognitive, behavioural, and physiological reactions, thus closely related to health. There is wide variety of ways for individuals to regulate their emotion. In this regard, there are two kinds of emotion regulation strategy; first Antecedent-focused emotion regulation consisting of situation selection, situation modification, attentional deployment, cognitive change and second, Response-focused emotion regulation consisting of suppression. The purpose of this research is to investigate personality factors relate with emotion regulation strategies. 339 students from Faculty of Psychology, Universitas Gadjah Mada were participating in this study and given The Big Five Personality Factors (Ramdhani, 2012, adaptation, and the modified version of the Emotion Regulation Scale was used, Emotion Regulation Questionnaire (John & Gross, 2004 which measure personality and emotion regulation respectively. Using multiple regression analysis, the study indicated that personality predicts emotion regulation strategies.

  5. [Ghrelin: beyond hunger regulation].

    Milke García, Maria del Pilar


    Man ingests food to mitigate hunger (mediated by physiological and biochemical signals), satisfy appetite (subjective sensation) and because of psychosocial reasons. Satiation biomarkers (stop feeding) are gastric distention and hormones (CCK, GLP-1) and satiety biomarkers (induce feeding) are food-induced thermogenesis, body temperature, glycaemia and also hormones (insulin, leptin and ghrelin). Oxidative metabolism/body composition, tryptophan/serotonin and proinflammatory cytokines are also implicated on hunger physiology. At the present time, ghrelin is the only known circulating orexigenic with potential on hunger/body weight regulation. It is a neuropeptide (endogenous ligand for the GH secretagogue) recently isolated from the oxyntic mucosa and synthesized mainly in the stomach. Its blood concentration depends on diet, hyperglucemia and adiposity/leptin. It is secreted 1-2 hours preprandially and its concentration decreases drastically during the postprandium. Ghrelin acts on the lateral hypothalamus and theoretically inhibits proinflammatory cytokine secretion and antagonizes leptin. Ghrelin physiologically increases food intake and stimulates adipogenesis, gastrointestinal motility and gastric acid secretion, and has other hormonal and cardiovascular functions. Ghrelin blood concentration is reduced in massive obesity, non-alcoholic steatohepatitis, polycystic ovary syndrome, acromegaly, hypogonadism, ageing, short bowel syndrome and rheumatoid arthritis; and increased in primary or secondary anorexia, starvation, chronic liver disease and celiac disease. Cerebral and peritoneal ghrelin administration (rats) and systemic administration (rats and healthy volunteers, cancer patients or patients on peritoneal dialysis) promotes food consumption and increases adiposity, of utmost importance in the treatment of patients with anorexia.

  6. Deciphering Transcriptional Regulation

    Valen, Eivind

    RNA); and ii) translation, in which the mRNA is translated into a protein. This thesis focus on the ¿rst of these steps, transcription, and speci¿cally the initiation of this. Simpli¿ed, initiation is preceded by the binding of several proteins, known as transcription factors (TFs), to DNA. This takes place......The myriad of cells in the human body are all made from the same blueprint: the human genome. At the heart of this diversity lies the concept of gene regulation, the process in which it is decided which genes are used where and when. Genes do not function as on/off buttons, but more like a volume...... control spanning the range from completely muted to cranked up to maximum. The volume, in this case, is the production rate of proteins. This production is the result of a two step procedure: i) transcription, in which a small part of DNA from the genome (a gene) is transcribed into an RNA molecule (an m...

  7. Regulating the sharing economy

    Kristofer Erickson


    Full Text Available In this introductory essay, we explore definitions of the ‘sharing economy’, a concept indicating both social (relational, communitarian and economic (allocative, profit-seeking aspects which appear to be in tension. We suggest combining the social and economic logics of the sharing economy to focus on the central features of network enabled, aggregated membership in a pool of offers and demands (for goods, services, creative expressions. This definition of the sharing economy distinguishes it from other related peer-to-peer and collaborative forms of production. Understanding the social and economic motivations for and implications of participating in the sharing economy is important to its regulation. Each of the papers in this special issue contributes to knowledge by linking the social and economic aspects of sharing economy practices to regulatory norms and mechanisms. We conclude this essay by suggesting future research to further clarify and render intelligible the sharing economy, not as a contradiction in terms but as an empirically observable realm of socio-economic activity.

  8. Epigenetic regulation in obesity.

    Drummond, Elaine M; Gibney, Eileen R


    Research suggests that 65% of variation in obesity is genetic. However, much of the known genetic associations have little known function and their effect size small, thus the gene-environment interaction, including epigenetic influences on gene expression, is suggested to be an important factor in the susceptibilty to obesity. This review will explore the potential of epigenetic markers to influence expression of genes associated with obesity. Epigenetic changes in utero are known to have direct implications on the phenotype of the offspring. More recently work has focused on how such epigenetic changes continue to regulate risk of obesity from infancy through to adulthood. Work has shown that, for example, hypomethylation of the MC4 gene causes an increase in expression, and has a direct impact on appetite and intake, and thus influences risk of obesity. Similar influences are also seen in other aspects of obesity including inflammation and adiposity. Maternal diet during foetal development has many epigenetic implications, which affect the offspring's risk factors for obesity during childhood and adulthood, and even in subsequent generations. Genes associated with risk of obesity, are susceptible to epigenetic mutations, which have subsequent effects on disease mechanisms, such as appetite and impaired glucose and insulin tolerance.

  9. Regulating regulatory T cells.

    Le, N T; Chao, N


    Regulatory T cells (Tregs) are a specialized subpopulation of T cells that act to suppress activation of other immune cells and thereby maintain immune system homeostasis, self-tolerance as well as control excessive response to foreign antigens. The mere concept of Tregs was the subject of significant controversy among immunologists for many years owing to the paucity of reliable markers for defining these cells and the ambiguity of the nature and molecular basis of suppressive phenomena. However, recent advances in the molecular characterization of this cell population have firmly established their existence and their vital role in the vertebrate immune system. Of interest, accumulating evidence from both humans and experimental animal models has implicated the involvement of Tregs in the development of graft-versus-host disease (GVHD). The demonstration that Tregs could separate GVHD from graft-versus-tumor (GVT) activity suggests that their immunosuppressive potential could be manipulated to reduce GVHD without detrimental consequence on GVT effect. Although a variety of T lymphocytes with suppressive capabilities have been reported, the two best-characterized subsets are the naturally arising, intrathymic-generated Tregs (natural Tregs) and the peripherally generated, inducible Tregs (inducible Tregs). This review summarizes our current knowledge of the generation, function and regulation of these two populations of Tregs during an immune response. Their role in the development of GVHD and their therapeutic potential for the prevention and treatment of GVHD will also be described.

  10. NCAM regulates cell motility.

    Prag, Søren; Lepekhin, Eugene A; Kolkova, Kateryna; Hartmann-Petersen, Rasmus; Kawa, Anna; Walmod, Peter S; Belman, Vadym; Gallagher, Helen C; Berezin, Vladimir; Bock, Elisabeth; Pedersen, Nina


    Cell migration is required during development of the nervous system. The regulatory mechanisms for this process, however, are poorly elucidated. We show here that expression of or exposure to the neural cell adhesion molecule (NCAM) strongly affected the motile behaviour of glioma cells independently of homophilic NCAM interactions. Expression of the transmembrane 140 kDa isoform of NCAM (NCAM-140) caused a significant reduction in cellular motility, probably through interference with factors regulating cellular attachment, as NCAM-140-expressing cells exhibited a decreased attachment to a fibronectin substratum compared with NCAM-negative cells. Ectopic expression of the cytoplasmic part of NCAM-140 also inhibited cell motility, presumably via the non-receptor tyrosine kinase p59(fyn) with which NCAM-140 interacts. Furthermore, we showed that the extracellular part of NCAM acted as a paracrine inhibitor of NCAM-negative cell locomotion through a heterophilic interaction with a cell-surface receptor. As we showed that the two N-terminal immunoglobulin modules of NCAM, which are known to bind to heparin, were responsible for this inhibition, we presume that this receptor is a heparan sulfate proteoglycan. A model for the inhibitory effect of NCAM is proposed, which involves competition between NCAM and extracellular components for the binding to membrane-associated heparan sulfate proteoglycan.

  11. Regulation of sphingomyelin metabolism.

    Bienias, Kamil; Fiedorowicz, Anna; Sadowska, Anna; Prokopiuk, Sławomir; Car, Halina


    Sphingolipids (SFs) represent a large class of lipids playing diverse functions in a vast number of physiological and pathological processes. Sphingomyelin (SM) is the most abundant SF in the cell, with ubiquitous distribution within mammalian tissues, and particularly high levels in the Central Nervous System (CNS). SM is an essential element of plasma membrane (PM) and its levels are crucial for the cell function. SM content in a cell is strictly regulated by the enzymes of SM metabolic pathways, which activities create a balance between SM synthesis and degradation. The de novo synthesis via SM synthases (SMSs) in the last step of the multi-stage process is the most important pathway of SM formation in a cell. The SM hydrolysis by sphingomyelinases (SMases) increases the concentration of ceramide (Cer), a bioactive molecule, which is involved in cellular proliferation, growth and apoptosis. By controlling the levels of SM and Cer, SMSs and SMases maintain cellular homeostasis. Enzymes of SM cycle exhibit unique properties and diverse tissue distribution. Disturbances in their activities were observed in many CNS pathologies. This review characterizes the physiological roles of SM and enzymes controlling SM levels as well as their involvement in selected pathologies of the Central Nervous System, such as ischemia/hypoxia, Alzheimer disease (AD), Parkinson disease (PD), depression, schizophrenia and Niemann Pick disease (NPD). Copyright © 2016 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  12. Regulatory and functional interactions of plant growth regulators and plant glutathione S-transferases (GSTs).

    Moons, Ann


    Plant glutathioneS-transferases (GSTs) are a heterogeneous superfamily of multifunctional proteins, grouped into six classes. The tau (GSTU) and phi (GSTF) class GSTs are the most represented ones and are plant-specific, whereas the smaller theta (GSTT) and zeta (GSTZ) classes are also found in animals. The lambda GSTs (GSTL) and the dehydroascorbate reductases (DHARs) are more distantly related. Plant GSTs perform a variety of pivotal catalytic and non-enzymatic functions in normal plant development and plant stress responses, roles that are only emerging. Catalytic functions include glutathione (GSH)-conjugation in the metabolic detoxification of herbicides and natural products. GSTs can also catalyze GSH-dependent peroxidase reactions that scavenge toxic organic hydroperoxides and protect from oxidative damage. GSTs can furthermore catalyze GSH-dependent isomerizations in endogenous metabolism, exhibit GSH-dependent thioltransferase safeguarding protein function from oxidative damage and DHAR activity functioning in redox homeostasis. Plant GSTs can also function as ligandins or binding proteins for phytohormones (i.e., auxins and cytokinins) or anthocyanins, thereby facilitating their distribution and transport. Finally, GSTs are also indirectly involved in the regulation of apoptosis and possibly also in stress signaling. Plant GST genes exhibit a diversity of expression patterns during biotic and abiotic stresses. Stress-induced plant growth regulators (i.e., jasmonic acid [JA], salicylic acid [SA], ethylene [ETH], and nitric oxide [NO] differentially activate GST gene expression. It is becoming increasingly evident that unique combinations of multiple, often interactive signaling pathways from various phytohormones and reactive oxygen species or antioxidants render the distinct transcriptional activation patterns of individual GSTs during stress. Underestimated post-transcriptional regulations of individual GSTs are becoming increasingly evident and roles

  13. Involvement of miR-30c in resistance to doxorubicin by regulating YWHAZ in breast cancer cells

    Fang, Y. [Department of Central Laboratory, The First Affiliated People’s Hospital, Jiangsu University, Zhenjiang, Jiangsu (China); Shen, H. [Department of Oncology, The First Affiliated People’s Hospital, Jiangsu University, Zhenjiang, Jiangsu (China); Cao, Y. [Department of Central Laboratory, The First Affiliated People’s Hospital, Jiangsu University, Zhenjiang, Jiangsu (China); Li, H. [Department of Central Laboratory, The Fourth Affiliated People’s Hospital, Jiangsu University, Zhenjiang, Jiangsu (China); Qin, R. [Department of Oncology, The First Affiliated People’s Hospital, Jiangsu University, Zhenjiang, Jiangsu (China); Chen, Q. [Department of Central Laboratory, The First Affiliated People’s Hospital, Jiangsu University, Zhenjiang, Jiangsu (China); Long, L. [Department of Oncology, The First Affiliated People’s Hospital, Jiangsu University, Zhenjiang, Jiangsu (China); Zhu, X.L. [Department of Central Laboratory, The Fourth Affiliated People’s Hospital, Jiangsu University, Zhenjiang, Jiangsu (China); Xie, C.J. [Department of Central Laboratory, The First Affiliated People’s Hospital, Jiangsu University, Zhenjiang, Jiangsu (China); Xu, W.L. [Department of Central Laboratory, The Fourth Affiliated People’s Hospital, Jiangsu University, Zhenjiang, Jiangsu (China)


    MicroRNAs (miRNAs) are small RNA molecules that modulate gene expression implicated in cancer, which play crucial roles in diverse biological processes, such as development, differentiation, apoptosis, and proliferation. The aim of this study was to investigate whether miR-30c mediated the resistance of breast cancer cells to the chemotherapeutic agent doxorubicin (ADR) by targeting tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ). miR-30c was downregulated in the doxorubicin-resistant human breast cancer cell lines MCF-7/ADR and MDA-MB-231/ADR compared with their parental MCF-7 and MDA-MB-231 cell lines, respectively. Furthermore, we observed that transfection of an miR-30c mimic significantly suppressed the ability of MCF-7/ADR to resist doxorubicin. Moreover, the anti-apoptotic gene YWHAZ was confirmed as a target of miR-30c by luciferase reporter assay, and further studies indicated that the mechanism for miR-30c on the sensitivity of breast cancer cells involved YWHAZ and its downstream p38 mitogen-activated protein kinase (p38MAPK) pathway. Together, our findings provided evidence that miR-30c was one of the important miRNAs in doxorubicin resistance by regulating YWHAZ in the breast cancer cell line MCF-7/ADR.

  14. 78 FR 31551 - Federal Acquisition Regulation; Submission for OMB Review; Commerce Patent Regulations


    ... Regulation; Submission for OMB Review; Commerce Patent Regulations AGENCIES: Department of Defense (DOD... approved information collection requirement concerning Department of Commerce patent regulations. A notice... Collection 9000- 0095, Commerce Patent Regulations, by any of the following methods: :...

  15. 76 FR 43585 - Bank Secrecy Act Regulations; Definitions and Other Regulations Relating to Money Services...


    ... Regulations; Definitions and Other Regulations Relating to Money Services Businesses AGENCY: Treasury... Proposed Rulemaking, Definitions and Other Regulations Relating to Money Services Businesses, 74 FR 22129... to the Bank Secrecy Act Regulations-- Definitions and Other Regulations Relating to Money...

  16. Glucocorticoid Regulation of Reproduction.

    Geraghty, Anna C; Kaufer, Daniela


    It is well accepted that stress, measured by increased glucocorticoid secretion, leads to profound reproductive dysfunction. In times of stress, glucocorticoids activate many parts of the fight or flight response, mobilizing energy and enhancing survival, while inhibiting metabolic processes that are not necessary for survival in the moment. This includes reproduction, an energetically costly procedure that is very finely regulated. In the short term, this is meant to be beneficial, so that the organism does not waste precious energy needed for survival. However, long-term inhibition can lead to persistent reproductive dysfunction, even if no longer stressed. This response is mediated by the increased levels of circulating glucocorticoids, which orchestrate complex inhibition of the entire reproductive axis. Stress and glucocorticoids exhibits both central and peripheral inhibition of the reproductive hormonal axis. While this has long been recognized as an issue, understanding the complex signaling mechanism behind this inhibition remains somewhat of a mystery. What makes this especially difficult is attempting to differentiate the many parts of both of these hormonal axes, and new neuropeptide discoveries in the last decade in the reproductive field have added even more complexity to an already complicated system. Glucocorticoids (GCs) and other hormones within the hypothalamic-pituitary-adrenal (HPA) axis (as well as contributors in the sympathetic system) can modulate the hypothalamic-pituitary-gonadal (HPG) axis at all levels-GCs can inhibit release of GnRH from the hypothalamus, inhibit gonadotropin synthesis and release in the pituitary, and inhibit testosterone synthesis and release from the gonads, while also influencing gametogenesis and sexual behavior. This chapter is not an exhaustive review of all the known literature, however is aimed at giving a brief look at both the central and peripheral effects of glucocorticoids on the reproductive function.

  17. Regulation of gas infrastructure expansion

    De Joode, J.


    The topic of this dissertation is the regulation of gas infrastructure expansion in the European Union (EU). While the gas market has been liberalised, the gas infrastructure has largely remained in the regulated domain. However, not necessarily all gas infrastructure facilities – such as gas storag

  18. Novel protein regulates ERK pathway


    @@ The ERK (extracellular signal-regulated kinase) pathway plays a critical role in the vital processes of living cells such as proliferation and differentiation.Recently, CAS scientists in Shanghai have discovered a novel mechanism of spatial regulation on ERK pathway. The result was published in the 4 September issue of the Proceedings of National Academy of Sciences(PNAS).

  19. Regulation of TRPML1 function.

    Waller-Evans, Helen; Lloyd-Evans, Emyr


    TRPML1 is a ubiquitously expressed cation channel found on lysosomes and late endosomes. Mutations in TRPML1 cause mucolipidosis type IV and it has been implicated in Alzheimer's disease and HIV. However, the mechanisms by which TRPML1 activity is regulated are not well understood. This review summarizes the current understanding of TRPML1 activation and regulation.

  20. Deceptive Business Practices: Federal Regulations.

    Rohrer, Daniel Morgan

    Federal regulations to prevent deceptive advertising seek to balance the advertiser's freedom of speech with protection of the consumer. This paper discusses what the Federal Trade Commission (FTC) has done to regulate advertising and evaluates the adequacy of its controls. The commission uses cease-and-desist orders, affirmative disclosure,…