Sample records for wln t6n dotj

  1. Modelling a water purification process for quality monitoring

    NARCIS (Netherlands)

    Meulen, van der F.H.; Luca, S.; Overal, G.; Dubbeldam, J.L.A.; Di Bucchianico, A.; Jongbloed, G.; Dubbeldam, J.; Groenevelt, W.; Heemink, A.W.; Lahaye, D.; Meerman, C.; Meulen, van der F.


    This paper deals with a quality engineering problem introduced by ‘Waterlaboratorium Noord’ (WLN) situated at the Netherlands. In-terest lies in determining an optimal sampling frequency that provides suÿcient information on the water quality in a drinking water purifica-tion plant. The water

  2. Flight Test Techniques. Proceedings of the Flight Mechanics Panel Symposium Held in Lisbon, Portugal on 2-5 April 1984 (United States)


    E103 3000 200 270090 1800 Fig. 7: Antenna Radiation Pattern Aircraft: D028. Type of Antenna: Monopole Frequency: 1.5 6Nz, Bank Angle: 0±Z Deg. Ref...Pattern: - - - Monopole Mr 70 250 F 4g. 8: S.uae1tpcl S0OA0r a) Test Emitter: Frequency Range UHF -18 GHz Antenna Parabolic Dish Wln Horn Feeds Transmitter...essais d’extinction et rallumages moteur, de flottement, etc.. Un effort sp~cial a 6t consacr6, Ai Istres, A Ia mice en place de moyens et dispositifs

  3. von Neumann entropy associated with the haldane exclusion statistics

    International Nuclear Information System (INIS)

    Rajagopal, A.K.


    We obtain the von Neumann entropy per state of the Haldane exclusion statistics with parameter g in terms of the mean occupation number bar n{wlnw-(1+w)ln(1+w)}, where w=(1-bar n). This reduces correctly to the well known expressions in the limiting cases of Bose (g=0) and Fermi (g=1) statistics. We have derived the second and third order fluctuations in the occupation numbers for arbitrary g. An elegant general duality relationship between the w factor associated with the particle and that associated with the hole at the reciprocal g is deduced along with the attendant relationship between the two respective entropies

  4. Study of disulfide reduction and alkyl chloroformate derivatization of plasma sulfur amino acids using gas chromatography–mass spectrometry

    Czech Academy of Sciences Publication Activity Database

    Švagera, Z.; Hanzlíková, D.; Šimek, Petr; Hušek, Petr


    Roč. 402, č. 9 (2012), s. 2953-2963 ISSN 1618-2642 R&D Projects: GA MZd NS9755; GA ČR GAP206/10/2401; GA ČR GA203/09/2014 Institutional research plan: CEZ:AV0Z50070508 Keywords : plasma amino acids * disulfide-reducing agents * trichloroacetic acid deproteinization Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 3.659, year: 2012

  5. Dynamic study of yeast species and Saccharomyces cerevisiae strains during the spontaneous fermentations of Muscat blanc in Jingyang, China. (United States)

    Wang, Chunxiao; Liu, Yanlin


    The evolution of yeast species and Saccharomyces cerevisiae genotypes during spontaneous fermentations of Muscat blanc planted in 1957 in Jingyang region of China was followed in this study. Using a combination of colony morphology on Wallerstein Nutrient (WLN) medium, sequence analysis of the 26S rDNA D1/D2 domain and 5.8S-ITS-RFLP analysis, a total of 686 isolates were identified at the species level. The six species identified were S. cerevisiae, Hanseniaspora uvarum, Hanseniaspora opuntiae, Issatchenkia terricola, Pichia kudriavzevii (Issatchenkia orientalis) and Trichosporon coremiiforme. This is the first report of T. coremiiforme as an inhabitant of grape must. Three new colony morphologies on WLN medium and one new 5.8S-ITS-RFLP profile are described. Species of non-Saccharomyces, predominantly H. opuntiae, were found in early stages of fermentation. Subsequently, S. cerevisiae prevailed followed by large numbers of P. kudriavzevii that dominated at the end of fermentations. Six native genotypes of S. cerevisiae were determined by interdelta sequence analysis. Genotypes III and IV were predominant. As a first step in exploring untapped yeast resources of the region, this study is important for monitoring the yeast ecology in native fermentations and screening indigenous yeasts that will produce wines with regional characteristics. Copyright © 2012 Elsevier Ltd. All rights reserved.

  6. Bilgi Ağları ve Kütüphanelerarası İşbirliği

    Directory of Open Access Journals (Sweden)

    Yaşar Tonta


    Full Text Available This paper is on library and Information networks and inter- library co-operation. Different types of networks, their features and struc- tures are briefly reviewed. Also mentioned are the local area netvvorks (LANs, whereby libraries can easily access national and intemational net- works such as JANET, EARN and BITNET. Among other things the func- tions of «intelligent gatevvays», online public access catalogues (OPAÇs and electrbnic mail facilities are explained. A few examples of interlibrary co- operation which have been originated from networks are given in the sec- ond part of the paper; the activities of the Centre for Bibliographic Infor­mation in Japan; the services of library co-operatives such as BLCMP and SWALCAP in the UK, and the way they work; and, the Linked Systems Project, which is the most advanced project of four networks (LC, WLN, RLIN and OCLC in this respect in the US.

  7. 27 CFR 9.201 - Sloughhouse. (United States)


    ... Wilton Road at the hamlet of Dillard, section 6, T6N, R7E (Elk Grove Quadrangle); then (9) Proceed northwest 3.1 miles on Wilton Road to its intersection with Grant Line Road at the hamlet of Sheldon...

  8. Identification and characterization of lactic acid bacteria and yeasts of PDO Tuscan bread sourdough by culture dependent and independent methods. (United States)

    Palla, Michela; Cristani, Caterina; Giovannetti, Manuela; Agnolucci, Monica


    Sourdough fermentation has been increasingly used worldwide, in accordance with the demand of consumers for tasty, natural and healthy food. The high diversity of lactic acid bacteria (LAB) and yeast species, detected in sourdoughs all over the world, may affect nutritional, organoleptic and technological traits of leavened baked goods. A wide regional variety of traditional sourdough breads, over 200 types, has been recorded in Italy, including special types selected as worthy of either Protected Geographical Indication (PGI) or Protected Designation of Origin (PDO), whose sourdough microbiota has been functionally and molecularly characterized. As, due to the very recent designation, the microbiota of Tuscan bread sourdough has not been investigated so far, the aim of the present work was to isolate and characterize the species composition of LAB and yeasts of PDO Tuscan bread sourdough by culture-independent and dependent methods. A total of 130 yeasts from WLN medium and 193 LAB from both mMRS and SDB media were isolated and maintained to constitute the germplasm bank of PDO Tuscan bread. Ninety six LAB from mMRS medium and 68 yeasts from WLN medium were randomly selected and molecularly identified by ARDRA (Amplified Ribosomal DNA Restriction Analysis) and PCR-RFLP analysis of the ITS region, respectively, and sequencing. The yeast identity was confirmed by 26S D1/D2 sequencing. All bacterial isolates showed 99% identity with Lactobacillus sanfranciscensis, 65 yeast isolates were identified as Candida milleri, and 3 as Saccharomyces cerevisiae. Molecular characterization of PDO Tuscan bread sourdough by PCR-DGGE confirmed such data. The distinctive tripartite species association, detected as the microbiota characterizing the sourdough used to produce PDO Tuscan bread, encompassed a large number of L. sanfranciscensis and C. milleri strains, along with a few of S. cerevisiae. The relative composition and specific physiological characteristics of such microbiota

  9. Reaction-diffusion controlled growth of complex structures (United States)

    Noorduin, Willem; Mahadevan, L.; Aizenberg, Joanna


    Understanding how the emergence of complex forms and shapes in biominerals came about is both of fundamental and practical interest. Although biomineralization processes and organization strategies to give higher order architectures have been studied extensively, synthetic approaches to mimic these self-assembled structures are highly complex and have been difficult to emulate, let alone replicate. The emergence of solution patterns has been found in reaction-diffusion systems such as Turing patterns and the BZ reaction. Intrigued by this spontaneous formation of complexity we explored if similar processes can lead to patterns in the solid state. We here identify a reaction-diffusion system in which the shape of the solidified products is a direct readout of the environmental conditions. Based on insights in the underlying mechanism, we developed a toolbox of engineering strategies to deterministically sculpt patterns and shapes, and combine different morphologies to create a landscape of hierarchical multi scale-complex tectonic architectures with unprecedented levels of complexity. These findings may hold profound implications for understanding, mimicking and ultimately expanding upon nature's morphogenesis strategies, allowing the synthesis of advanced highly complex microscale materials and devices. WLN acknowledges the Netherlands Organization for Scientific Research for financial support

  10. Inference of viscosity jump at 670 km depth and lower mantle viscosity structure from GIA observations (United States)

    Nakada, Masao; Okuno, Jun'ichi; Irie, Yoshiya


    A viscosity model with an exponential profile described by temperature (T) and pressure (P) distributions and constant activation energy (E_{{{um}}}^{{*}} for the upper mantle and E_{{{lm}}}^* for the lower mantle) and volume (V_{{{um}}}^{{*}} and V_{{{lm}}}^*) is employed in inferring the viscosity structure of the Earth's mantle from observations of glacial isostatic adjustment (GIA). We first construct standard viscosity models with an average upper-mantle viscosity ({\\bar{η }_{{{um}}}}) of 2 × 1020 Pa s, a typical value for the oceanic upper-mantle viscosity, satisfying the observationally derived three GIA-related observables, GIA-induced rate of change of the degree-two zonal harmonic of the geopotential, {\\dot{J}_2}, and differential relative sea level (RSL) changes for the Last Glacial Maximum sea levels at Barbados and Bonaparte Gulf in Australia and for RSL changes at 6 kyr BP for Karumba and Halifax Bay in Australia. Standard viscosity models inferred from three GIA-related observables are characterized by a viscosity of ˜1023 Pa s in the deep mantle for an assumed viscosity at 670 km depth, ηlm(670), of (1 - 50) × 1021 Pa s. Postglacial RSL changes at Southport, Bermuda and Everglades in the intermediate region of the North American ice sheet, largely dependent on its gross melting history, have a crucial potential for inference of a viscosity jump at 670 km depth. The analyses of these RSL changes based on the viscosity models with {\\bar{η }_{{{um}}}} ≥ 2 × 1020 Pa s and lower-mantle viscosity structures for the standard models yield permissible {\\bar{η }_{{{um}}}} and ηlm (670) values, although there is a trade-off between the viscosity and ice history models. Our preferred {\\bar{η }_{{{um}}}} and ηlm (670) values are ˜(7 - 9) × 1020 and ˜1022 Pa s, respectively, and the {\\bar{η }_{{{um}}}} is higher than that for the typical value of oceanic upper mantle, which may reflect a moderate laterally heterogeneous upper

  11. Development of Multiple Antibiotic Resistance in Bacillus subtilis Cells Exposed to Microgravity: the BRIC-18 Experiment to the International Space Station (United States)

    Fajardo-Cavazos, Patricia; Moeller, Ralf; Nicholson, Wayne; Narvel, Raed

    Increased pathogenicity of opportunistic bacteria during long-term spaceflight is considered an astronaut risk. Because only a limited pharmacy can be carried on long-duration missions, the development of resistance to multiple antibiotics is a concern for mission planning. In support of the BRIC-18 experiment to the ISS, we have performed ground-based experiments to address the question whether simulated microgravity affects the frequency of resistance to the model antibiotics rifampicin (RFM) and trimethoprim (TMP). In these experiments, the model bacteria Bacillus subtilis and Staphylococcus epidermidis were cultivated for 6 days at ISS ambient temperature in 10-ml High Aspect Ratio Vessels (HARVs) on two 4-place clinostats (Synthecon) oriented either vertically (V) or horizontally (H). Cells were harvested, enumerated and plated onto medium containing RFM (5 micrograms/ml). The frequency of mutation to RFM resistance was calculated, and RFM-resistant mutants were plated onto medium containing the second antibiotic, TMP (5 micrograms/ml) to determine the frequency of mutation to double (RFM+TMP) resistance. After 6 days of cultivation, V-cultures showed higher cell densities and than H-cultures for both bacteria. However, only in B. subtilis did V-cultures show higher frequencies of mutation to RFM resistance than H-cultures. Launch of BRIC-18 to the ISS is currently scheduled for March 16, 2014 and return 30 days later. Results from both the spaceflight and ground control experiments will be presented. Supported by NASA-SAIP fellowship to R.N. and NASA grant (NNX12AN70G) to P.F.-C., R.M., and W.L.N.

  12. Distribution of Dekkera bruxellensis in a sugarcane-based fuel ethanol fermentation plant. (United States)

    da Silva, T C D; Leite, F C B; De Morais, M A


    We investigated the presence of the yeast Dekkera bruxellensis in samples collected at three points surrounding the industrial alcoholic fermentation plants of two distilleries where there are often cases of contamination caused by this yeast: this involved sugar cane wash water, feeding sugar cane juice and vinasse from the treatment pond. Total yeast was isolated in WLN medium with bromocresol green and cycloheximide and further selected on the basis of its ability to grow in synthetic medium containing nitrate. Following this, colonies were selected from the distribution on nitrate plates and identified by amplification with species-specific primers and DNA sequencing of the 26S-D1/D2 locus. The results showed that D. bruxellensis is introduced through the feeding substrate, which suggests that its cells originated with the harvested cane. Subsequently, its population circulates as a result of the reuse of water for washing the cane, in a continuous re-inoculation of the plant with yeasts. Furthermore, the yeast population is formed in the vinasse by the addition of wash water into the treatment ponds and then reintroduced to the culture fields by fertigation, so that the process can be renewed in the following season. It is now possible to adopt sanitation procedures that can prevent the entry of the contamination to the fermentation process. The presence of the yeast Dekkera bruxellensis is sometimes attributed to a decline in the industrial productivity of ethanol since it has a more limited fermentation capacity than Saccharomyces cerevisiae. Although its adaptability to the industrial environment has been noted, so far, there has been no evidence to determine the source of this contamination. In this study, we provide evidence to show that D. bruxellensis comes from the fields together with the harvested cane and is then accumulated and recirculated. It might be possible to prevent the accumulation of this yeast by carrying out sanitation controls during

  13. Sharp Nose Lens Design Using Refractive Index Gradient (United States)


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  14. Lower extremity robotic exoskeleton training: Case studies for complete spinal cord injury walking. (United States)

    Lemaire, Edward D; Smith, Andrew J; Herbert-Copley, Andrew; Sreenivasan, Vidya


    Recent advances in exoskeleton technology has made lower extremity powered exoskeletons (LEPE) a viable treatment tool to restore upright walking mobility to persons with spinal cord injury (SCI). Evaluate ARKE exoskeleton training within a rehabilitation centre environment. Case studies are presented for two male participants, age 41 and 30, motor complete SCI at T6 (N01) and T12 (N02), respectively, as they progress from new LEPE users to independent walking. The ARKE 2.0 LEPE (Bionik Laboratories Inc., Toronto, Canada) was used for all training (hip and knee powered, forearm crutches, control tablet). Data were collected on session times, activity metrics from ARKE system logs, and qualitative questionnaire feedback. N01 required 18, 30-minute training sessions to achieve independent walking. N01 walked independently within the 12 session target. Foot strikes were frequently before the end of the programmed swing phase, which were handled by the ARKE control system. Subjective ratings of LEPE learning, comfort, pain, fatigue, and overall experience were high for sitting-standing and moderate to high for walking. This reflected the complexity of learning to safely walk. Qualitative feedback supported the continuation of LEPE use in rehabilitation settings based on end-user desire for upright mobility.

  15. Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosa Transport of bovine oocytes in maturation medium without a controlled gaseous atmosphere

    Directory of Open Access Journals (Sweden)

    Fábio Gallas Leivas


    Full Text Available Oócitos (n=1177 bovinos obtidos da aspiração de folículos com diâmetro entre 2 e 8mm, de ovários de matadouro foram divididos aleatoriamente em quatro tratamentos com 11 repetições. Os oócitos foram maturados por 24h em TCM-199 Sais de Earle, acrescido de 25mM de bicarbonato de sódio, 25mM de HEPES, rFHS-h, Soro de Vaca em Estro (SVE e piruvato, em estufa a 39ºC, com 5% de CO2 em ar e umidade saturada (Grupo Controle, n=296 ou, submetidos ao transporte simulado por 6 (T6, n=286, 12 (T12, n=294 ou 18h (T18, n=301 em meio de maturação TCM+HEPES, em banho-maria a 39ºC, com os mesmos componentes utilizados para o Grupo Controle, porém com apenas 1mM de bicarbonato. Decorrido cada período de transporte, os mesmos foram transferidos para placas com meio de maturação, completando o período de 24h em estufa, nas mesmas condições do Grupo Controle. O período de fecundação foi de 18h em condições semelhantes de temperatura e atmosfera gasosa, em FERT-TALP acrescido de heparina, sendo a dose inseminante de 1x106 espermatozóides/mL, selecionados por migração ascendente. Os prováveis zigotos foram cultivados em meio SOF + 5% SVE por 8 dias, em estufa a 39ºC, em bolsas gaseificadas com 5% CO2, 5% O2 e 90% N2. Na avaliação da clivagem, não houve diferença entre os tratamentos. As taxas de desenvolvimento embrionário no dia 7 foram semelhantes para os grupos Controle (20,9%, T6 (19,2% e T12 (21,4%, com uma redução (P0,05 na taxa de eclosão. O número médio de células dos blastocistos eclodidos não diferiu (P>0,05 entre os grupos Controle (136, T6 (125,5 e T12 (126,8. Esses resultados indicam a possibilidade do transporte de oócitos bovinos em meio de maturação TCM+HEPES, sem controle da atmosfera gasosa, a 39ºC, pelo período de até 12h. Esta técnica oferece uma alternativa prática e eficiente para o transporte dos oócitos bovinos destinados à produção in vitro de embriões bovinos (PIV.Oocytes (n=1177

  16. Força de mordida em crianças com mantenedor de espaço funcional na fase da dentadura mista inicial Bite force in children with functional space maintainer in early mixed dentition

    Directory of Open Access Journals (Sweden)

    Suzane Rodrigues Jacinto-Gonçalves


    Full Text Available OBJETIVO: verificar a influência do mantenedor de espaço funcional (MEF na força muscular em crianças com perda prematura de molares decíduos na fase inicial da dentadura mista (5,5 a 6,5 anos de idade com oclusão normal, considerando-se o padrão facial. MÉTODOS: a amostra foi constituída por dois grupos: Grupo MEF (n = 15, com perda precoce de pelo menos um molar decíduo; e Grupo Controle (n = 16. Determinou-se a força de mordida máxima (FM com um tubo transmissor pressurizado, conectado a um circuito eletrônico analógico/digital antes (t0, um mês (t1 e 6 meses (t6 após a instalação do mantenedor. O padrão facial foi determinado pelo índice de Jarabak (FHR. Os dados foram analisados pela estatística descritiva, análise de variância para medidas repetidas, teste t e correlação de Pearson. RESULTADOS:a FM foi significativamente menor em t0 e t1 para o Grupo MEF em relação ao Controle. Em t6 não houve diferença. Os dois grupos apresentaram a FM significativamente maior em t6, em relação a t0, mas o percentual de variação para o Grupo MEF foi significativamente maior. Os valores de FHR não se correlacionaram com a FM, não ocorrendo diferença entre os tipos faciais intragrupos. Os dolicofaciais do Grupo MEF apresentaram a FM significativamente menor que os do Grupo Controle, em t0 e t1, mas não em t6. CONCLUSÃO: o maior aumento na FM para o Grupo MEF após 6 meses foi favorecido pelo maior número de contatos oclusais, devido aos dentes artificiais, melhorando a função e recuperando a força muscular.AIM: To verify the influence of a functional space maintainer (FSM in muscle strength in children with premature loss of primary molars in early mixed dentition (5.5-6.5 years old with normal occlusion, considering the facial pattern. METHODS: The sample was distributed in: FSM Group (n = 15, with premature loss of at least one primary molar and a Control Group (n = 16. It was determined the maximal bite force (BF