Sample records for wind nebula msh

  1. High-energy X-ray imaging of the pulsar wind nebula MSH 15-52: constraints on particle acceleration and transport

    DEFF Research Database (Denmark)

    An, Hongjun; Madsen, Kristin K.; Reynolds, Stephen P.


    We present the first images of the pulsar wind nebula (PWN) MSH 15−52 in the hard X-ray band (8 keV), as measured with the Nuclear Spectroscopic Telescope Array (NuSTAR). Overall, the morphology of the PWN as measured by NuSTAR in the 3–7 keV band is similar to that seen in Chandra high-resolutio......We present the first images of the pulsar wind nebula (PWN) MSH 15−52 in the hard X-ray band (8 keV), as measured with the Nuclear Spectroscopic Telescope Array (NuSTAR). Overall, the morphology of the PWN as measured by NuSTAR in the 3–7 keV band is similar to that seen in Chandra high......-resolution imaging. However, the spatial extent decreases with energy, which we attribute to synchrotron energy losses as the particles move away from the shock. The hard-band maps show a relative deficit of counts in the northern region toward the RCW 89 thermal remnant, with significant asymmetry.We find...

  2. High-Energy X-Ray Imaging of the Pulsar Wind Nebula MSH 15-52: Constraints on Particle Acceleration and Transport (United States)

    An, Hongjun; Madsen, Kristin K.; Reynolds, Stephen P.; Kaspi, Victoria M.; Harrison, Fiona A.; Boggs, Steven E.; Christensen, Finn E.; Craig, William W.; Fryer, Chris L.; Grefenstette, Brian W.; hide


    We present the first images of the pulsar wind nebula (PWN) MSH 15-52 in the hard X-ray band (8 keV), as measured with the Nuclear Spectroscopic Telescope Array (NuSTAR). Overall, the morphology of the PWN as measured by NuSTAR in the 3-7 keV band is similar to that seen in Chandra high-resolution imaging. However, the spatial extent decreases with energy, which we attribute to synchrotron energy losses as the particles move away from the shock. The hard-band maps show a relative deficit of counts in the northern region toward the RCW 89 thermal remnant, with significant asymmetry. We find that the integrated PWN spectra measured with NuSTAR and Chandra suggest that there is a spectral break at 6 keV, which may be explained by a break in the synchrotron emitting electron distribution at approximately 200 TeV and/or imperfect cross calibration. We also measure spatially resolved spectra, showing that the spectrum of the PWN softens away from the central pulsar B1509-58, and that there exists a roughly sinusoidal variation of spectral hardness in the azimuthal direction. We discuss the results using particle flow models. We find non-monotonic structure in the variation with distance of spectral hardness within 50 of the pulsar moving in the jet direction, which may imply particle and magnetic-field compression by magnetic hoop stress as previously suggested for this source. We also present two-dimensional maps of spectral parameters and find an interesting shell-like structure in the N(sub H) map. We discuss possible origins of the shell-like structure and their implications.

  3. Modelling pulsar wind nebulae

    CERN Document Server


    In view of the current and forthcoming observational data on pulsar wind nebulae, this book offers an assessment of the theoretical state of the art of modelling them. The expert authors also review the observational status of the field and provide an outlook for future developments. During the last few years, significant progress on the study of pulsar wind nebulae (PWNe) has been attained both from a theoretical and an observational perspective, perhaps focusing on the closest, more energetic, and best studied nebula: the Crab, which appears in the cover. Now, the number of TeV detected PWNe is similar to the number of characterized nebulae observed at other frequencies over decades of observations. And in just a few years, the Cherenkov Telescope Array will increase this number to several hundreds, actually providing an essentially complete account of TeV emitting PWNe in the Galaxy. At the other end of the multi-frequency spectrum, the SKA and its pathfinder instruments, will reveal thousands of new pulsa...

  4. Turbulent Magnetic Relaxation in Pulsar Wind Nebulae (United States)

    Zrake, Jonathan; Arons, Jonathan


    We present a model for magnetic energy dissipation in a pulsar wind nebula. A better understanding of this process is required to assess the likelihood that certain astrophysical transients may be powered by the spin-down of a “millisecond magnetar.” Examples include superluminous supernovae, gamma-ray bursts, and anticipated electromagnetic counterparts to gravitational wave detections of binary neutron star coalescence. Our model leverages recent progress in the theory of turbulent magnetic relaxation to specify a dissipative closure of the stationary magnetohydrodynamic (MHD) wind equations, yielding predictions of the magnetic energy dissipation rate throughout the nebula. Synchrotron losses are self-consistently treated. To demonstrate the model’s efficacy, we show that it can reproduce many features of the Crab Nebula, including its expansion speed, radiative efficiency, peak photon energy, and mean magnetic field strength. Unlike ideal MHD models of the Crab (which lead to the so-called σ-problem), our model accounts for the transition from ultra to weakly magnetized plasma flow and for the associated heating of relativistic electrons. We discuss how the predicted heating rates may be utilized to improve upon models of particle transport and acceleration in pulsar wind nebulae. We also discuss implications for the Crab Nebula’s γ-ray flares, and point out potential modifications to models of astrophysical transients invoking the spin-down of a millisecond magnetar.

  5. High energy neutrinos from pulsar wind nebulae (United States)

    Di Palma, Irene


    Several Pulsar Wind Nebulae have been detected in the TeV band in the last decade.The TeV emission is typically interpreted in a purely leptonic scenario, but this usually requires that the magnetic field in the Nebula be much lower than the equipartition value and the assumption of an enhanced target radiation at IR frequencies. In this work we consider the possibility that, in addition to the relativistic electrons, also relativistic hadrons are present in these nebulae. Assuming that part of the emitted TeV photons are of hadronic origin, we compute the associated flux of ˜ 1 - 100 TeV neutrinos. We use the IceCube non detection to put constraints on the fraction of TeV photons that might be contributed by hadrons and estimate the number of neutrino events that can be expected from these sources in IceCube, ANTARES and in KM3Net.

  6. Pulsar wind nebulae created by fast-moving pulsars (United States)

    Kargaltsev, O.; Pavlov, G. G.; Klingler, N.; Rangelov, B.


    We review multiwavelength properties of pulsar wind nebulae created by supersonically moving pulsars and the effects of pulsar motion on the pulsar wind nebulae morphologies and the ambient medium. Supersonic pulsar wind nebulae are characterized by bow-shaped shocks around the pulsar and/or cometary tails filled with the shocked pulsar wind. In the past several years significant advances in supersonic pulsar wind nebula studies have been made in deep observations with the Chandra and XMM-Newton X-ray observatories and the Hubble Space Telescope. In particular, these observations have revealed very diverse supersonic pulsar wind nebula morphologies in the pulsar vicinity, different spectral behaviours of long pulsar tails, the presence of puzzling outflows misaligned with the pulsar velocity and far-UV bow shocks. Here we review the current observational status focusing on recent developments and their implications.

  7. Pulsar Wind Nebulae and Cosmic Rays: A Bedtime Story

    Energy Technology Data Exchange (ETDEWEB)

    Weinstein, A.


    The role pulsar wind nebulae play in producing our locally observed cosmic ray spectrum remains murky, yet intriguing. Pulsar wind nebulae are born and evolve in conjunction with SNRs, which are favored sites of Galactic cosmic ray acceleration. As a result they frequently complicate interpretation of the gamma-ray emission seen from SNRs. However, pulsar wind nebulae may also contribute directly to the local cosmic ray spectrum, particularly the leptonic component. This paper reviews the current thinking on pulsar wind nebulae and their connection to cosmic ray production from an observational perspective. It also considers how both future technologies and new ways of analyzing existing data can help us to better address the relevant theoretical questions. A number of key points will be illustrated with recent results from the VHE (E > 100 GeV) gamma-ray observatory VERITAS.

  8. Gamma-rays from pulsar wind nebulae in starburst galaxies (United States)

    Mannheim, Karl; Elsässer, Dominik; Tibolla, Omar


    Recently, gamma-ray emission at TeV energies has been detected from the starburst galaxies NGC253 (Acero et al., 2009) [1] and M82 (Acciari et al., 2009) [2]. It has been claimed that pion production due to cosmic rays accelerated in supernova remnants interacting with the interstellar gas is responsible for the observed gamma rays. Here, we show that the gamma-ray pulsar wind nebulae left behind by the supernovae contribute to the TeV luminosity in a major way. A single pulsar wind nebula produces about ten times the total luminosity of the Sun at energies above 1 TeV during a lifetime of 105 years. A large number of 3 × 104 pulsar wind nebulae expected in a typical starburst galaxy at a distance of 4 Mpc can readily produce the observed TeV gamma rays.

  9. Multiband nonthermal radiative properties of pulsar wind nebulae (United States)

    Zhu, Bo-Tao; Zhang, Li; Fang, Jun


    Aims: The nonthermal radiative properties of 18 pulsar wind nebulae (PWNe) are studied in the 1D leptonic model. Methods: The dynamical and radiative evolution of a PWN in a nonradiative supernova remnant are self-consistently investigated in this model. The leptons (electrons/positrons) are injected with a broken power-law form, and nonthermal emission from a PWN is mainly produced by time-dependent relativistic leptons through synchrotron radiation and inverse Compton process. Results: Observed spectral energy distributions (SEDs) of all 18 PWNe are reproduced well, where the indexes of low-energy electron components lie in the range of 1.0-1.8 and those of high-energy electron components in the range of 2.1-3.1. Our results show that FX/Fγ > 10 for young PWNe; 1 age negatively correlate with X-ray luminosity, the ratio of X-ray to gamma-ray luminosities, and the synchrotron luminosity.

  10. Multiwavelength Studies of the Mouse Pulsar Wind Nebula (United States)

    Klingler, Noel; Kargaltsev, Oleg; Pavlov, George G.; Ng, C.-Y.; Beniamini, Paz; O'Sullivan, Samantha


    PSR J1747-2958 is a young and energetic pulsar at an estimated distance of ~5 kpc. It is moving supersonically through the ISM and powers the famous Mouse pulsar wind nebula (PWN; G359.23-0.82): a tail spanning 45" in X-rays and 12' in radio. We discuss the results of our analysis of deep Chandra observations (as well as archival radio and IR data) of the Mouse PWN. We present a spatially-resolved spectral map of the PWN, which displays a photon index which varies strongly with distance from the pulsar over the 45" extent of the X-ray tail as the result of synchrotron cooling. We discuss the shape of the multiwavelength spectrum, the PWN physical properties (e.g., we infer a high magnetic field B~200 μG), and the connection between PWN morphology and radio/gamma-ray light curves which we use to constrain the viewing angle and identify structures in the PWN. We compare the Mouse pulsar with the population of other pulsars with measured (or inferred) velocities.

  11. Detection of gamma-ray emission from the Vela pulsar wind nebula with AGILE. (United States)

    Pellizzoni, A; Trois, A; Tavani, M; Pilia, M; Giuliani, A; Pucella, G; Esposito, P; Sabatini, S; Piano, G; Argan, A; Barbiellini, G; Bulgarelli, A; Burgay, M; Caraveo, P; Cattaneo, P W; Chen, A W; Cocco, V; Contessi, T; Costa, E; D'Ammando, F; Del Monte, E; De Paris, G; Di Cocco, G; Di Persio, G; Donnarumma, I; Evangelista, Y; Feroci, M; Ferrari, A; Fiorini, M; Fuschino, F; Galli, M; Gianotti, F; Hotan, A; Labanti, C; Lapshov, I; Lazzarotto, F; Lipari, P; Longo, F; Marisaldi, M; Mastropietro, M; Mereghetti, S; Moretti, E; Morselli, A; Pacciani, L; Palfreyman, J; Perotti, F; Picozza, P; Pittori, C; Possenti, A; Prest, M; Rapisarda, M; Rappoldi, A; Rossi, E; Rubini, A; Santolamazza, P; Scalise, E; Soffitta, P; Striani, E; Trifoglio, M; Vallazza, E; Vercellone, S; Verrecchia, F; Vittorini, V; Zambra, A; Zanello, D; Giommi, P; Colafrancesco, S; Antonelli, A; Salotti, L; D'Amico, N; Bignami, G F


    Pulsars are known to power winds of relativistic particles that can produce bright nebulae by interacting with the surrounding medium. These pulsar wind nebulae are observed by their radio, optical, and x-ray emissions, and in some cases also at TeV (teraelectron volt) energies, but the lack of information in the gamma-ray band precludes drawing a comprehensive multiwavelength picture of their phenomenology and emission mechanisms. Using data from the AGILE satellite, we detected the Vela pulsar wind nebula in the energy range from 100 MeV to 3 GeV. This result constrains the particle population responsible for the GeV emission and establishes a class of gamma-ray emitters that could account for a fraction of the unidentified galactic gamma-ray sources.

  12. Fermi-LAT Constraints on the Pulsar Wind Nebula Nature of HESS J1857+026 (United States)

    Rousseau, R.; Grondin, M.-H.; VanEtten, A.; Lemoine-Goumard, M.; Bogdanov, S.; Hessels, J. W. T.; Kaspi, V. M.; Arzoumanian, Z.; Camilo, F.; Casandjian, J. M.; hide


    Since its launch, the Fermi satellite has firmly identified 5 pulsar wind nebulae plus a large number of candidates, all powered by young and energetic pulsars. HESS J1857+026 is a spatially extended gamma-ray source detected by H.E.S.S. and classified as a possible pulsar wind nebula candidate powered by PSR J1856+0245. Aims. We search for -ray pulsations from PSR J1856+0245 and explore the characteristics of its associated pulsar wind nebula. Methods. Using a rotational ephemeris obtained from the Lovell telescope at Jodrell Bank Observatory at 1.5 GHz, we phase.fold 36 months of gamma-ray data acquired by the Large Area Telescope (LAT) aboard Fermi. We also perform a complete gamma-ray spectral and morphological analysis. Results. No pulsation was detected from PSR J1856+0245. However, significant emission is detected at a position coincident with the TeV source HESS J1857+026. The gamma-ray spectrum is well described by a simple power law with a spectral index of Gamma = 1.53 +/- 0.11(sub stat) +/- 0.55(sub syst) and an energy flux of G(0.1 C100 GeV) = (2.71 +/- 0.52(sub stat) +/- 1.51(sub syst) X 10(exp -11) ergs/ sq cm/s. This implies a gamma.ray efficiency of approx 5 %, assuming a distance of 9 kpc, the gamma-ray luminosity of L(sub gamma) (sub PWN) (0.1 C100 GeV) = (2.5 +/- 0.5(sub stat) +/- 1.5(sub syst)) X 10(exp 35)(d/(9kpc))(exp 2) ergs/s and E-dot = 4.6 X 10(exp 36) erg /s, in the range expected for pulsar wind nebulae. Detailed multi-wavelength modeling provides new constraints on its pulsar wind nebula nature.

  13. Constraining the Turbulence Scale and Mixing of a Crushed Pulsar Wind Nebula (United States)

    Ng, Chi Yung; Ma, Y. K.; Bucciantini, Niccolo; Slane, Patrick O.; Gaensler, Bryan M.; Temim, Tea


    Pulsar wind nebulae (PWNe) are synchrotron-emitting nebulae resulting from the interaction between pulsars' relativistic particle outflows and the ambient medium. The Snail PWN in supernova remnant G327.1-1.1 is a rare system that has recently been crushed by supernova reverse shock. We carried out radio polarization observations with the Australia Telescope Compact Array and found highly ordered magnetic field structure in the nebula. This result is surprising, given the turbulent environment expected from hydrodynamical simulations. We developed a toymodel and compared simple simulations with observations to constrain the characteristic turbulence scale in the PWN and the mixing with supernova ejecta. We estimate that the turbulence scale is about one-eighth to one-sixth of the nebula radius and a pulsar wind filling factor of 50-75%. The latter implies substantial mixing of the pulsar wind with the surrounding supernova ejecta.This work is supported by an ECS grant of the Hong Kong Government under HKU 709713P. The Australia Telescope is funded by the Commonwealth of Australia for operation as a National Facility managed by CSIRO.

  14. Diffusion in pulsar wind nebulae: an investigation using magnetohydrodynamic and particle transport models (United States)

    Porth, O.; Vorster, M. J.; Lyutikov, M.; Engelbrecht, N. E.


    We study the transport of high-energy particles in pulsar wind nebulae (PWN) using three-dimensional magnetohydrodynamic (MHD) and test-particle simulations, as well as a Fokker-Planck particle transport model. The latter includes radiative and adiabatic losses, diffusion, and advection on the background flow of the simulated MHD nebula. By combining the models, the spatial evolution of flux and photon index of the X-ray synchrotron emission is modelled for the three nebulae G21.5-0.9, the inner regions of Vela, and 3C 58, thereby allowing us to derive governing parameters: the magnetic field strength, average flow velocity, and spatial diffusion coefficient. For comparison, the nebulae are also modelled with the semi-analytic Kennel & Coroniti model but the Porth et al. model generally yields better fits to the observational data. We find that high velocity fluctuations in the turbulent nebula (downstream of the termination shock) give rise to efficient diffusive transport of particles, with average Péclet number close to unity, indicating that both advection and diffusion play an important role in particle transport. We find that the diffusive transport coefficient of the order of ˜ 2 × 1027(Ls/0.42 Ly) cm2 s- 1 (Ls is the size of the termination shock) is independent of energy up to extreme particle Lorentz factors of γp ˜ 1010.

  15. Discovery of a Pulsar Wind Nebula Candidate in the Cygnus Loop (United States)

    Katsuda, Satoru; Tsunemi, Hiroshi; Mori, Koji; Uchida, Hiroyuki; Petre, Robert; Yamada, Shin'ya; Tamagawa, Toru


    We report on a discovery of a diffuse nebula containing a point-like source in the southern blowout region of the Cygnus Loop supernova remnant, based on Suzaku and XMM-Newton observations. The X-ray spectra from the nebula and the point-like source are well represented by an absorbed power-law model with photon indices of 2.2+/-0.1 and 1.6+/-0.2, respectively. The photon indices as well as the flux ratio of F(sub nebula)/F(sub point-like) approx. 4 lead us to propose that the system is a pulsar wind nebula, although pulsations have not yet been detected. If we attribute its origin to the Cygnus Loop supernova, then the 0.5-8 keV luminosity of the nebula is computed to be 2.1x10(exp 31)(d/540pc)(exp 2)ergss/2, where d is the distance to the Loop. This implies a spin-down loss-energy E approx. 2.6x10(exp 35)(d/540pc)(exp 2)ergs/s. The location of the neutron star candidate, approx.2deg away from the geometric center of the Loop, implies a high transverse velocity of approx.1850(theta/2deg)(d/540pc)(t/10kyr)/k/s assuming the currently accepted age of the Cygnus Loop.

  16. Spatially-resolved Spectroscopy of the IC443 Pulsar Wind Nebula and Environs (United States)

    Swartz, D. A.; Weisskopf, M. C.; Zavlin, V. E.; Bucciantini, N.; Clarke, T. E.; Karovska, M.; Pavlov, G. G.; O'Dell, S. L.; vanderHorst, A J.; Yukita, M.


    Deep Chandra ACIS observations of the region around the putative pulsar, CXOU J061705.3+222117, in the supernova remnant IC443 reveal, for the first time, a ring-like morphology surrounding the pulsar and a jet-like structure oriented roughly north-south across the ring and through the pulsar location. The observations further confirm that (1) the spectrum and flux of the central object are consistent with a rotation-powered pulsar interpretation, (2) the non-thermal surrounding nebula is likely powered by the pulsar wind, and (3) the thermal-dominated spectrum at greater distances is consistent with emission from the supernova remnant. The cometary shape of the nebula, suggesting motion towards the southwest (or, equivalently, flow of ambient medium to the northeast), appears to be subsonic; there is no evidence for a strong bow shock, and the circular ring is not distorted by motion through the ambient medium.

  17. The Geminga pulsar wind nebula in the mid-infrared and submillimetre (United States)

    Greaves, J. S.; Holland, W. S.


    The nearby middle-aged Geminga pulsar has crossed the Galactic plane within the last ∼0.1 Myr. We present archival data from Wide-field Infrared Survey Explorer and from SCUBA and SCUBA-2 on the James Clerk Maxwell Telescope to assess whether any mid-infrared and submillimetre emission arises from interaction of the pulsar wind nebula with the interstellar medium. A candidate shell and bow shock are reported. Given the low pulsar velocity and local density, dust grains appear able to penetrate into the nebula. A compact source seen towards the pulsar is fitted with a dust spectrum. If confirmed as a real association at higher resolution, this could be a circum-pulsar disc of at least a few Earth-masses, in which future planets could form.

  18. Relativistic MHD modeling of magnetized neutron stars, pulsar winds, and their nebulae (United States)

    Del Zanna, L.; Pili, A. G.; Olmi, B.; Bucciantini, N.; Amato, E.


    Neutron stars are among the most fascinating astrophysical sources, being characterized by strong gravity, densities about the nuclear one or even above, and huge magnetic fields. Their observational signatures can be extremely diverse across the electromagnetic spectrum, ranging from the periodic and low-frequency signals of radio pulsars, up to the abrupt high-energy gamma-ray flares of magnetars, where energies of ∼ {10}46 {erg} are released in a few seconds. Fast-rotating and highly magnetized neutron stars are expected to launch powerful relativistic winds, whose interaction with the supernova remnants gives rise to the non-thermal emission of pulsar wind nebulae, which are known cosmic accelerators of electrons and positrons up to PeV energies. In the extreme cases of proto-magnetars (magnetic fields of ∼ {10}15 G and millisecond periods), a similar mechanism is likely to provide a viable engine for the still mysterious gamma-ray bursts. The key ingredient in all these spectacular manifestations of neutron stars is the presence of strong magnetic fields in their constituent plasma. Here we will present recent updates of a couple of state-of-the-art numerical investigations by the high-energy astrophysics group in Arcetri: a comprehensive modeling of the steady-state axisymmetric structure of rotating magnetized neutron stars in general relativity, and dynamical 3D MHD simulations of relativistic pulsar winds and their associated nebulae.

  19. Near-Infrared, Kilosecond Variability of the Wisps And Jet in the Crab Pulsar Wind Nebula

    Energy Technology Data Exchange (ETDEWEB)

    Melatos, Andrew; Scheltus, D.; /Melbourne U.; Whiting, M.T.; /New South Wales U.; Eikenberry, S.S.; /Florida U.; Romani, R.W.; /Stanford U., Phys. Dept.; Rigaut, F.; /Gemini; Spitkovsky, A.; /KIPAC, Menlo Park; Arons, J.; /UC, Berkeley, Astron. Dept.; Payne, D.J.B.; /Melbourne U.


    We present a time-lapse sequence of 20 near-infrared (J- and K'-band) snapshots of the central 20'' x 20'' of the Crab pulsar wind nebula, taken at sub-arcsecond resolution with the Hokupa'a/QUIRC adaptive optics camera on the Gemini North Telescope, and sampled at intervals of 10 minutes and 24 hours. It is observed that the equatorial wisps and polar knots in the termination shock of the pulsar wind appear to fluctuate in brightness on kilosecond time-scales. Maximum flux variations of {+-}24 {+-} 4 and {+-}14 {+-} 4 per cent relative to the mean (in 1.2 ks) are measured for the wisps and knots respectively, with greatest statistical significance in J band where the nebula background is less prominent. The J and K' flux densities imply different near-infrared spectra for the nonthermal continuum emission from the wisps and outermost polar knot (''sprite''), giving F{sub {nu}} {proportional_to} {nu}{sup -0.56{+-}0.12} and F{sub {nu}} {proportional_to} {nu}{sup -0.21{+-}0.13} respectively. The data are compared with existing optical and UV photometry and applied to constrain theories of the variability of the wisps (relativistic ion-cyclotron instability) and knots (relativistic fire hose instability).

  20. Halo Emission of the Cat's Eye Nebula, NGC 6543 Shock Excitation by Fast Stellar Winds

    Directory of Open Access Journals (Sweden)

    Siek Hyung


    Full Text Available Images taken with the Chandra X-ray telescope have for the the first time revealed the central, wind-driven, hot bubble (Chu et al. 2001, while Hubble Space Telescope (HST WFPC2 images of the Cat's Eye nebula, NGC 6543, show that the temperature of the halo region of angular radius ~ 20'', is much higher than that of the inner bright H II region. With the coupling of a photoionization calculation to a hydrodynamic simulation, we predict the observed [O III] line intensities of the halo region with the same O abundance as in the core H II region: oxygen abundance gradient does not appear to exist in the NGC 6543 inner halo. An interaction between a (leaky fast stellar wind and halo gas may cause the higher excitation temperatures in the halo region and the inner hot bubble region observed with the Chandra X-ray telescope.

  1. DA 495: An Aging Pulsar Wind Nebula with Possible TeV Gamma-Ray Counterpart (United States)

    Coerver, Anna; Mori, Kaya; Hailey, Charles; Gotthelf, Eric; Mukherjee, Reshmi; Hui, Michelle; Dingus, Brenda; Goodman, Jordan; NuSTAR, HAWC, VERITAS


    A pulsar wind nebula is created when a high-mass star collapses and forms an isolated neutron star surrounded by a relativistic particle wind. DA 495 is thought to be such an object, although no pulsations have been detected. DA 495 was first detected in the radio band and is characterized by an annular radio morphology with a radio emission dip in the center. It has been theorized that the wind nebula has an unusually high magnetization of 1.3 mG, and the age of the PWN has been estimated from the 1.3 GHz energy break to be approximately 20 kyr. With such a high wind magnetization, one would expect synchrotron radiation to fall off fairly quickly, yet NuSTAR (the Nuclear Spectroscopic Telescope Array) detected hard X-rays at energies greater than 10 keV, and a study of the Galactic Plane by the HAWC very-high-energy (VHE) gamma-ray telescope revealed a source coincident with DA 495 at energies greater than 10 TeV. This source was also detected at TeV energies by the VERITAS gamma-ray telescope. DA 495 was detected in the X-ray up to 10 keV by both Chandra and XMM-Newton X-ray telescopes before NuSTAR performed follow-up observations of the region in June 2017. DA 495 is one of the first TeV sources observed by the NuSTAR-HAWC-VERITAS Galactic Legacy collaboration. We carried out spectral and imaging analysis of both NuSTAR and Chandra data on DA 495 and will present results of our study of DA 495.


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    Swartz, Douglas A.; Zavlin, Vyacheslav E. [USRA, Astrophysics Office, NASA Marshall Space Flight Center, ZP12, Huntsville, AL 35812 (United States); Pavlov, George G. [Department of Astronomy and Astrophysics, Pennsylvania State University, 525 Davey Lab, University Park, PA 16802 (United States); Clarke, Tracy [Remote Sensing Division, Code 7213, Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC (United States); Castelletti, Gabriela [Instituto de Astronomía y Física del Espacio (IAFE, CONICET-UBA), CC67, Suc. 28, 1428, Buenos Aires (Argentina); Bucciantini, Niccolò [INAF—Osservatorio Astrofisico di Arcetri, L. go E. Fermi 5, I-50125 Firenze (Italy); Karovska, Margarita [Smithsonian Astrophysical Observatory, MS 4, 60 Garden Street, Cambridge, MA 02138 (United States); Horst, Alexander J. van der [Department of Physics, The George Washington University, 725 21 Street NW, Washington, DC 20052 (United States); Yukita, Mihoko [The Johns Hopkins University, Homewood Campus, Baltimore, MD 21218 (United States); Weisskopf, Martin C. [Astrophysics Office, NASA Marshall Space Flight Center, ZP12, Huntsville, AL 35812 (United States)


    Deep Chandra ACIS observations of the region around the putative pulsar, CXOU J061705.3+222127, in the supernova remnant (SNR) IC 443 reveal an ∼5″ radius ring-like structure surrounding the pulsar and a jet-like feature oriented roughly north–south across the ring and through the pulsar's location at 06{sup h}17{sup m}5.{sup s}200 + 22°21′27.″52 (J2000.0 coordinates). The observations further confirm that (1) the spectrum and flux of the central object are consistent with a rotation-powered pulsar, (2) the non-thermal spectrum and morphology of the surrounding nebula are consistent with a pulsar wind, and (3) the spectrum at greater distances is consistent with thermal emission from the SNR. The cometary shape of the nebula, suggesting motion toward the southwest, appears to be subsonic: There is no evidence either spectrally or morphologically for a bow shock or contact discontinuity; the nearly circular ring is not distorted by motion through the ambient medium; and the shape near the apex of the nebula is narrow. Comparing this observation with previous observations of the same target, we set a 99% confidence upper limit to the proper motion of CXOU J061705.3+222127 to be less than 44 mas yr{sup −1} (310 km s{sup −1} for a distance of 1.5 kpc), with the best-fit (but not statistically significant) projected direction toward the west.

  3. Evolution of a Pulsar Wind Nebula within a Composite Supernova Remnant (United States)

    Kolb, Christopher; Blondin, John; Slane, Patrick; Temim, Tea


    The interaction between a pulsar wind nebula (PWN) and its host supernova remnant (SNR) can produce a vast array of observable structures. Asymmetry present within these structures derives from the complexity of the composite system, where many factors take turns playing a dominating hand throughout the stages of composite SNR evolution. Of particular interest are systems characterized by blastwave expansion within a nonuniform interstellar medium (ISM), which contain an active pulsar having a substantial “kick” velocity (upward of 300 {km} {{{s}}}-1), because these systems tend to produce complex morphologies. We present a numerical model that employs these and several other factors in an effort to generate asymmetry similar to that seen in various X-ray and radio observations. We find that the main parameters driving structure are ISM uniformity and total pulsar spin-down energy, with secondary contributions from factors such as pulsar trajectory and initial spin-down luminosity. We also investigate the dynamics behind PWN “tails,” which may form to link active pulsars to a crushed, relic nebula as the reverse shock passes. We find that the directions of such tails are not good indicators of pulsar motion, but direction does reveal the flow of ejecta created by the passage of a reverse shock.

  4. The Asymmetric Bow Shock/Pulsar Wind Nebula of PSR J2124–3358 (United States)

    Romani, Roger W.; Slane, Patrick; Green, Andrew W.


    We describe new measurements of the remarkable Hα/UV/X-ray bow shock and pulsar wind nebula (PWN) of the isolated millisecond pulsar (MSP) PSR J2124‑3358. Chandra X-ray Observatory imaging shows a one-sided jet structure with a softer equatorial outflow. KOALA integral field unit spectroscopy shows that non-radiative emission dominates the bow shock and that the Hα nebula is asymmetric about the pulsar velocity with an elongation into the plane of the sky. We extend analytic models of the contact discontinuity to accommodate such shapes and compare these to the data. Using Hubble Space Telescope UV detections of the pulsar and bow shock, radio timing distance, proper motion measurements, and the CXO-detected projected spin axis, we model the 3D PWN momentum flux distribution. The integrated momentum flux depends on the ionization of the ambient ISM, but for an expected ambient warm neutral medium, we infer I=2.4× {10}45 {{g}} {{cm}}2. This implies {M}{NS}=1.6{--}2.1 {M}ȯ , depending on the equation of state, which in turn suggests that the MSP gained significant mass during recycling and then lost its companion. However, this conclusion is at present tentative, since lower ionization allows ∼ 30 % lower masses, and uncertainty in the parallax allows up to 50% error.

  5. Pulsar Wind Nebulae with Bow Shocks: Non-thermal Radiation and Cosmic Ray Leptons (United States)

    Bykov, A. M.; Amato, E.; Petrov, A. E.; Krassilchtchikov, A. M.; Levenfish, K. P.


    Pulsars with high spin-down power produce relativistic winds radiating a non-negligible fraction of this power over the whole electromagnetic range from radio to gamma-rays in the pulsar wind nebulae (PWNe). The rest of the power is dissipated in the interactions of the PWNe with the ambient interstellar medium (ISM). Some of the PWNe are moving relative to the ambient ISM with supersonic speeds producing bow shocks. In this case, the ultrarelativistic particles accelerated at the termination surface of the pulsar wind may undergo reacceleration in the converging flow system formed by the plasma outflowing from the wind termination shock and the plasma inflowing from the bow shock. The presence of magnetic perturbations in the flow, produced by instabilities induced by the accelerated particles themselves, is essential for the process to work. A generic outcome of this type of reacceleration is the creation of particle distributions with very hard spectra, such as are indeed required to explain the observed spectra of synchrotron radiation with photon indices Γ≲ 1.5. The presence of this hard spectral component is specific to PWNe with bow shocks (BSPWNe). The accelerated particles, mainly electrons and positrons, may end up containing a substantial fraction of the shock ram pressure. In addition, for typical ISM and pulsar parameters, the e+ released by these systems in the Galaxy are numerous enough to contribute a substantial fraction of the positrons detected as cosmic ray (CR) particles above few tens of GeV and up to several hundred GeV. The escape of ultrarelativistic particles from a BSPWN—and hence, its appearance in the far-UV and X-ray bands—is determined by the relative directions of the interstellar magnetic field, the velocity of the astrosphere and the pulsar rotation axis. In this respect we review the observed appearance and multiwavelength spectra of three different types of BSPWNe: PSR J0437-4715, the Guitar and Lighthouse nebulae, and

  6. Mapping supernova remnants and pulsar wind nebulae across decades of energy (United States)

    Hewitt, John W.


    Ground- and space-based gamma ray observatories of the past decade have given us a new understanding of particle accelerators in our galaxy. The improved spatial resolution and sensitivity of recent gamma-ray surveys of the Galactic plane have resolved confusion of sources identified numerous sources to study the physics of particle acceleration and the diffusion of energetic particles into the galaxy. Here I highlight some recent studies of Galactic accelerators from GeV to TeV energies, that allow us to disentangle hadronic from leptonic emission, constrain cosmic ray diffusion, and measure the conditions of particle acceleration. Supernova remnants and pulsar wind nebulae are found to be the two most common Galactic sources identified in very high energy gamma rays, and the future capabilities of CTA promise a dramatic increase in our knowledge of these classes which are currently limited to only a few of the most well-studied cases.

  7. Gamma-ray and TeV Emission Properties of Pulsars and Pulsar Wind Nebulae (United States)

    de Jager, O. C.


    Introduction: Although more than 1,600 radio pulsars have been discovered, only a few have been detected in the gamma-ray band. This is not because they are intrinsically faint, but because the pulsed component seems to cut off below about 30 GeV (the EGRET range), where the sensitivity was severely limited. However, ground-based atmospheric Cerenkov telescopes operating above 100 GeV (the Very High Energy or VHE domain), have both good sensitivity and good angular resolution to resolve several pulsar wind nebulae (PWN) in the VHE gamma-ray domain. Methods: This will be a review talk summarising the progress to date on pulsar and pulsar wind nebula observations and theory. Results and Conclusions: Since gamma-ray observations below 30 GeV have been limited by poor sensitivity, an instrument like GLAST should be able to resolve the pulsed component of a significant fraction of radio pulsars. This talk will show how the discovery potential of GLAST will be limited for fainter sources in the absence of contemporary radio pulsar parameters. This calls for the introduction of wide field-of-view radio pulsar monitors like KAT to resolve this problem. Most progress on PWN in the gamma-ray domain was made by the HESS telescope system in Namibia. In this case we progressed to the level where VHE Gamma-ray Astronomy is taking the lead at all wavelengths (radio, IR, optical, X-ray and gamma-ray) in the identification and understanding of new PWN. We will show how the spin history of the PWN is more relevant to such VHE observations rather than X-rays, although the latter probe the more recent history of PWN evolution. We will then show how these complementary wavebands can be combined to obtain new information about aspects such as the birth periods of pulsars and conversion efficiency of spin-down power to injected ultra-relativistic electrons.


    Energy Technology Data Exchange (ETDEWEB)

    Kastner, J. H.; Montez, R. Jr.; Rapson, V. [Center for Imaging Science and Laboratory for Multiwavelength Astrophysics, Rochester Institute of Technology, 54 Lomb Memorial Drive, Rochester, NY 14623 (United States); Balick, B. [Department of Astronomy, University of Washington, Seattle, WA (United States); Frew, D. J.; De Marco, O.; Parker, Q. A. [Department of Physics and Astronomy and Macquarie Research Centre for Astronomy, Astrophysics and Astrophotonics, Macquarie University, Sydney, NSW 2109 (Australia); Miszalski, B. [South African Astronomical Observatory, P.O. Box 9, Observatory, 7935 (South Africa); Sahai, R. [Jet Propulsion Laboratory, California Institute of Technology, MS 183-900, Pasadena, CA 91109 (United States); Blackman, E.; Frank, A. [Department of Physics and Astronomy, University of Rochester, Rochester, NY (United States); Chu, Y.-H. [Department of Astronomy, University of Illinois, Champagne-Urbana, IL (United States); Guerrero, M. A. [Instituto de Astrofisica de Astronomia, Glorieta de la Astronomia s/n, Granada 18008 (Spain); Lopez, J. A. [Instituto de Astronomia, Universidad Nacional Autonoma de Mexico, Campus Ensenada, Apdo. Postal 22860, Ensenada, B. C. (Mexico); Zijlstra, A. [School of Physics and Astronomy, University of Manchester, Manchester M13 9PL (United Kingdom); Behar, E. [Department of Physics, Technion (Israel); Bujarrabal, V. [Observatorio Astronomico Nacional, Apartado 112, E-28803, Alcala de Henares (Spain); Corradi, R. L. M. [Instituto de Astrofisica de Canarias, E-38200 La Laguna, Tenerife (Spain); Nordhaus, J. [Center for Computational Relativity and Gravitation, Rochester Institute of Technology, Rochester, NY 14623 (United States); Sandin, C., E-mail:, E-mail:, E-mail: [Leibniz Institute for Astrophysics Potsdam (AIP), An der Sternwarte 16, D-14482 Potsdam (Germany); and others


    We present an overview of the initial results from the Chandra Planetary Nebula Survey (CHANPLANS), the first systematic (volume-limited) Chandra X-Ray Observatory survey of planetary nebulae (PNe) in the solar neighborhood. The first phase of CHANPLANS targeted 21 mostly high-excitation PNe within {approx}1.5 kpc of Earth, yielding four detections of diffuse X-ray emission and nine detections of X-ray-luminous point sources at the central stars (CSPNe) of these objects. Combining these results with those obtained from Chandra archival data for all (14) other PNe within {approx}1.5 kpc that have been observed to date, we find an overall X-ray detection rate of {approx}70% for the 35 sample objects. Roughly 50% of the PNe observed by Chandra harbor X-ray-luminous CSPNe, while soft, diffuse X-ray emission tracing shocks-in most cases, 'hot bubbles'-formed by energetic wind collisions is detected in {approx}30%; five objects display both diffuse and point-like emission components. The presence (or absence) of X-ray sources appears correlated with PN density structure, in that molecule-poor, elliptical nebulae are more likely to display X-ray emission (either point-like or diffuse) than molecule-rich, bipolar, or Ring-like nebulae. All but one of the point-like CSPNe X-ray sources display X-ray spectra that are harder than expected from hot ({approx}100 kK) central stars emitting as simple blackbodies; the lone apparent exception is the central star of the Dumbbell nebula, NGC 6853. These hard X-ray excesses may suggest a high frequency of binary companions to CSPNe. Other potential explanations include self-shocking winds or PN mass fallback. Most PNe detected as diffuse X-ray sources are elliptical nebulae that display a nested shell/halo structure and bright ansae; the diffuse X-ray emission regions are confined within inner, sharp-rimmed shells. All sample PNe that display diffuse X-ray emission have inner shell dynamical ages {approx}< 5 Multiplication

  9. Multi-wavelength observations of pulsar wind nebulae and composite supernova remnants (United States)

    Temim, Tea

    Multi-wavelength studies of pulsar wind nebulae (PWNe) and supernova remnants (SNRs) lead to a better understanding of their evolutionary development, the interaction of supernovae (SNe) and pulsar winds with their surroundings, and nucleosynthesis and production and processing of dust grains by SNe. PWNe and composite supernova remnants, in particular, are unique laboratories for the study of the energetic pulsar winds, particle injection processes, and the impact of PWNe on the evolving SNR. They provide information on SNR shock properties, densities and temperatures, and the chemical composition and the ionization state of the material ejected by SNe. SNRs also serve as laboratories for the study of dust production and processing in SNe. While X-ray observations yield important information about the SN progenitor, hot gas properties, SN explosion energy, and the surrounding interstellar medium (ISM), the IR can provide crucial information about the faint non-thermal emission, continuum emission from dust, and forbidden line emission from SN ejecta. Combining observations at a wide range of wavelengths provides a more complete picture of the SNR development and helps better constrain current models describing a SNR's evolution and its impact on the surrounding medium. This thesis focuses on a multi-wavelength study of PWNe in various stages of their evolution and investigates their interaction with the expanding SN ejecta and dust and the SNR reverse shock. The study of these interactions can provide important information on the SNR properties that may otherwise be unobservable. The work in this thesis has been carried out under the supervision of Patrick Slane at the Harvard-Smithsonian Center for Astrophysics, and Charles E. Woodward and Rebert D. Gehrz at the University of Minnesota. The first part of the thesis summarizes the evolution and observational properties of SNRs and PWNe, with a focus on the evolution of young PWNe that are sweeping up inner SN

  10. Human MSH2 (hMSH2) protein controls ATP processing by hMSH2-hMSH6. (United States)

    Heinen, Christopher D; Cyr, Jennifer L; Cook, Christopher; Punja, Nidhi; Sakato, Miho; Forties, Robert A; Lopez, Juana Martin; Hingorani, Manju M; Fishel, Richard


    The mechanics of hMSH2-hMSH6 ATP binding and hydrolysis are critical to several proposed mechanisms for mismatch repair (MMR), which in turn rely on the detailed coordination of ATP processing between the individual hMSH2 and hMSH6 subunits. Here we show that hMSH2-hMSH6 is strictly controlled by hMSH2 and magnesium in a complex with ADP (hMSH2(magnesium-ADP)-hMSH6). Destabilization of magnesium results in ADP release from hMSH2 that allows high affinity ATP binding by hMSH6, which then enhances ATP binding by hMSH2. Both subunits must be ATP-bound to efficiently form a stable hMSH2-hMSH6 hydrolysis-independent sliding clamp required for MMR. In the presence of magnesium, the ATP-bound sliding clamps remain on the DNA for ∼8 min. These results suggest a precise stepwise kinetic mechanism for hMSH2-hMSH6 functions that appears to mimic G protein switches, severely constrains models for MMR, and may partially explain the MSH2 allele frequency in Lynch syndrome or hereditary nonpolyposis colorectal cancer.

  11. Radio Observations of the Pulsar Wind Nebula HESS J1303-631 with ATCA (United States)

    Sushch, Iurii; Oya, Igor; Schwanke, Ullrich; Johnston, Simon; Dalton, Matthew


    Based on its enregy-dependent morphology the initially unidentified very high energy (VHE; E > 100 GeV) gamma-ray source HESS J1303-631 was recently associated with the pulsar PSR J1301-6305. Subsequent detection of X-ray and GeV counterparts further supports the identification of the H.E.S.S. source as evolved pulsar wind nebula (PWN). Recent radio observations of the PSR J1301-6305 region with ATCA dedicated to search for the radio counterpart of HESS J1303-631 are reported here. Observations at 5.5 GHz and 7.5 GHz do not reveal any extended emission associated with the pulsar. The analysis of the archival 1.384 GHz and 2.368 GHz data also does not show any significant emission. The 1.384 GHz data reveal a hint of an extended shell-like emission in the same region which might be a supernova remnant. The implications of the non-detection at radio wavelengths on the nature and evolution of the PWN as well as the possibility of the SNR candidate being a birth place of PSR J1301-6305 are discussed.

  12. Molecular environment, reverberation, and radiation from the pulsar wind nebula in CTA 1 (United States)

    Martín, Jonatan; Torres, Diego F.; Pedaletti, Giovanna


    We estimate the molecular mass around CTA 1 using data from Planck and the Harvard CO survey. We observe that the molecular mass in the vicinity of the complex is not enough to explain the TeV emission observed by VERITAS, even under favorable assumptions for the cosmic ray acceleration properties of the supernova remnant. This supports the idea that the TeV emission comes from the pulsar wind nebula (PWN). Here, we model the spectrum of the PWN at possible different stages of its evolution, including both the dynamics of the PWN and the supernova remnant and their interaction via the reverse shock. We have included in the model the energy lost via radiation by particles and the particles escape when computing the pressure produced by the gas. This leads to an evolving energy partition, since for the same instantaneous sharing of the injection of energy provided by the rotational power, the field and the particles are affected differently by radiation and losses. We present the model, and study in detail how the spectrum of a canonical isolated PWN is affected during compression and re-expansion and how this may impact on the CTA 1 case. By exploring the phase-space of parameters that lead to radii in agreement with those observed, we then analyse different situations that might represent the current stage of the CTA 1 PWN, and discuss caveats and requirements of each one.

  13. Time-Dependent Electron Acceleration in Pulsar Wind Termination Shocks: Application the 2011 April Crab Nebula Gamma-ray Flare (United States)

    Kroon, John; Becker, Peter A.; Finke, Justin


    The strongest gamma-ray flare from the Crab nebula observed by Fermi-LAT took place in 2011 April. Emission (up to a few GeV) exceeded the quiescent flux level by more than an order of magnitude. The Crab nebula gamma-ray flares challenge classical particle acceleration models in pulsar wind nebulae, because the radiating electrons have energies exceeding the classical radiation-reaction limit for synchrotron. However, numerical simulations have suggested that the classical synchrotron limit can be exceeded if electrons experience shock-driven electrostatic acceleration due to magnetic reconnection. In this talk, I present and summarize a new time-dependent model based on a transport equation that accounts for electrostatic acceleration, synchrotron losses, and particle escape. We implement a “blob” paradigm in which magnetically confined electrons from the upstream pulsar wind encounter and cross through the termination shock, producing a flare. We show that our model can reproduce the gamma-ray spectra and the integrated light curve for the 2011 April event, and we find that electrostatic acceleration occurs on both sides of the termination shock, driven by magnetic reconnection. We conclude that the dominant mode of particle escape changes from diffusive escape to advective escape as the blob passes through the shock.

  14. Modeling the effect of small-scale magnetic turbulence on the X-ray properties of Pulsar Wind Nebulae (United States)

    Bucciantini, N.; Bandiera, R.; Olmi, B.; Del Zanna, L.


    Pulsar Wind Nebulae (PWNe) constitute an ideal astrophysical environment to test our current understanding of relativistic plasma processes. It is well known that magnetic fields play a crucial role in their dynamics and emission properties. At present, one of the main issues concerns the level of magnetic turbulence present in these systems, which in the absence of space resolved X-ray polarization measures cannot be directly constrained. In this work, we investigate, for the first time using simulated synchrotron maps, the effect of a small-scale fluctuating component of the magnetic field on the emission properties in X-ray. We illustrate how to include the effects of a turbulent component in standard emission models for PWNe and which consequences are expected in terms of net emissivity and depolarization, showing that the X-ray surface brightness maps can provide already some rough constraints. We then apply our analysis to the Crab and Vela nebulae and by comparing our model with Chandra and Vela data, we found that the typical energies in the turbulent component of the magnetic field are about 1.5-3 times the one in the ordered field.

  15. The VELA-X-Pulsar Wind Nebula Revisited with Four Years of Fermi Large Area Telescope Observations (United States)

    Grondin, M. -H.; Romani, R. W.; Lemoine-Goumard, M.; Guillemot, L.; Harding, Alice K.; Reposeur, T.


    The Vela supernova remnant (SNR) is the closest SNR to Earth containing an active pulsar, the Vela pulsar (PSR B0833-45). This pulsar is an archetype of the middle-aged pulsar class and powers a bright pulsar wind nebula (PWN), Vela-X, spanning a region of 2deg × 3deg south of the pulsar and observed in the radio, X-ray, and very high energy ?-ray domains. The detection of the Vela-X PWN by the Fermi Large Area Telescope (LAT) was reported in the first year of the mission. Subsequently, we have reinvestigated this complex region and performed a detailed morphological and spectral analysis of this source using 4 yr of Fermi-LAT observations. This study lowers the threshold for morphological analysis of the nebula from 0.8 GeV to 0.3 GeV, allowing for the inspection of distinct energy bands by the LAT for the first time. We describe the recent results obtained on this PWN and discuss the origin of the newly detected spatial features.

  16. Discovery of TeV gamma-ray emission from the pulsar wind nebula 3C 58 by MAGIC

    Directory of Open Access Journals (Sweden)

    López-Coto Rubén


    Full Text Available The pulsar wind nebula (PWN 3C 58 is one of the historical very-high-energy (VHE; E>100 GeV gamma-ray source candidates. It has been compared to the Crab Nebula due to their morphological similarities. This object was detected by Fermi-LAT with a spectrum extending beyond 100 GeV. We analyzed 81 hours of 3C 58 data taken with the MAGIC telescopes and we detected VHE gamma-ray emission for the first time at TeV energies with a significance of 5.7 sigma and an integral flux of 0.65% C.U. above 1 TeV. According to our results 3C 58 is the least luminous PWN ever detected at VHE and the one with the lowest flux at VHE to date. We compare our results with the expectations of time-dependent models in which electrons up-scatter photon fields. The best representation favors a distance to the PWN of 2 kpc and Far Infrared (FIR comparable to CMB photon fields. Hadronic contribution from the hosting supernova remnant (SNR requires unrealistic energy budget given the density of the medium, disfavoring cosmic ray acceleration in the SNR as origin of the VHE gamma-ray emission.

  17. Studies of Pulsar Wind Nebula in the Supernova Remnant IC443: Preliminary Observations from the Chandra Data (United States)

    Ariyibi, E. A.


    Preliminary observations of the Chandra data were made in order to study the Pulsar Wind Nebula in the Supernova Remnant IC443. The Chandra X-ray observatory short observation on IC443 was centred on 13 chip ACIS. The CIAO analytical programme was used for the data analysis. The data were separated into point source, with an energy range of 2.1 to 10.0 keV, and diffuse source with energy less than 2.1 Kev. The resulting spectra were fitted to a power law. The observed density numbers and the normalised counts of both the point source and the diffuse source were used to describe the X-ray source. Afin d'étudier la "Pulsar wind Nebula" dans le reste de la Supernova IC 443, nous avons mené une exploitation préliminaire des observations provenant du satellite spatiale Chandra. L'observation brêve de IC 443, par Chandra fut centrée sur les composantes du spectromètre identifiées par la séquence 13. Le programme informatique CIAO fut utilisé pour l'analyse des données. Les données furent groupées en sources ponctuelles, chacune ayant des énergies allant de 2.1 a 10.0 kev ; et en sources diffuses chacune avec des énergies de moins de 2.1 kev. Les spectres obtenus furent interpolés à l'aide de fonction puissance. La densité de flux ainsi que le décompte des particules induites au détecteur par le rayonnement provenant des sources ponctuelles et diffuses furent utilisés pour décrire la source de rayon-X.


    Energy Technology Data Exchange (ETDEWEB)

    Matheson, H.; Safi-Harb, S. [Department of Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, R3T 2N2 (Canada); Kothes, R., E-mail:, E-mail:, E-mail: [Dominion Radio Astrophysical Observatory, National Research Council Herzberg, P.O. Box 248, Penticton, British Columbia, V2A 6J9 (Canada)


    Pulsar wind nebulae (PWNe) studies with the Chandra X-Ray Observatory have opened a new window to address the physics of pulsar winds, zoom on their interaction with their hosting supernova remnant (SNR) and interstellar medium, and identify their powering engines. We here present a new 70 ks, plus an archived 18 ks, Chandra ACIS observation of the SNR CTB 87 (G74.9+1.2), classified as a PWN with unusual radio properties and poorly studied in X-rays. We find that the peak of the X-ray emission is clearly offset from the peak of the radio emission by {approx}100'' and located at the southeastern edge of the radio nebula. We detect a point source-the putative pulsar-at the peak of the X-ray emission and study its spectrum separately from the PWN. This new point source, CXOU J201609.2+371110, is surrounded by a compact nebula displaying a torus-like structure and possibly a jet. A more extended diffuse nebula is offset from the radio nebula, extending from the point source to the northwest for {approx}250''. The spectra of the point source, compact nebula, and extended diffuse nebula are all well described by a power-law model with a photon index of 1.1 (0.7-1.6), 1.2 (0.9-1.4), and 1.7 (1.5-1.8), respectively, for a column density N{sub H} = 1.38 (1.21-1.57) Multiplication-Sign 10{sup 22} cm{sup -2} (90% confidence). The total X-ray luminosity of the source is {approx}1.6 Multiplication-Sign 10{sup 34} erg s{sup -1} at an assumed distance of 6.1 kpc, with {approx}2% and 6% contribution from the point source and compact nebula, respectively. The observed properties suggest that CTB 87 is an evolved ({approx}5-28 kyr) PWN, with the extended radio emission likely a ''relic'' PWN, as in Vela-X and G327.1-1.1. To date, however, there is no evidence for thermal X-ray emission from this SNR, and the SNR shell is still missing, suggesting expansion into a low-density medium (n{sub 0} < 0.2 D{sup -1/2}{sub 6.1} cm{sup -3}), likely


    Energy Technology Data Exchange (ETDEWEB)

    Kothes, R.; Foster, T. J. [National Research Council Herzberg, Dominion Radio Astrophysical Observatory, P.O. Box 248, Penticton, British Columbia, V2A 6J9 (Canada); Sun, X. H. [Sydney Institute for Astronomy, School of Physics, The University of Sydney, NSW 2006 (Australia); Reich, W., E-mail: [Max-Planck-Institut für Radioastronomie, Auf dem Hügel 69, D-53121 Bonn (Germany)


    We report the discovery of the new pulsar wind nebula (PWN) G141.2+5.0 in data observed with the Dominion Radio Astrophysical Observatory's Synthesis Telescope at 1420 MHz. The new PWN has a diameter of about 3.'5, which translates to a spatial extent of about 4 pc at a distance of 4.0 kpc. It displays a radio spectral index of α ≈ –0.7, similar to the PWN G76.9+1.1. G141.2+5.0 is highly polarized up to 40% with an average of 15% in the 1420 MHz data. It is located in the center of a small spherical H I bubble, which is expanding at a velocity of 6 km s{sup –1} at a systemic velocity of v {sub LSR} = –53 km s{sup –1}. The bubble could be the result of the progenitor star's mass loss or the shell-type supernova remnant (SNR) created by the same supernova explosion in a highly advanced stage. The systemic LSR velocity of the bubble shares the velocity of H I associated with the Cygnus spiral arm, which is seen across the second and third quadrants and an active star-forming arm immediately beyond the Perseus arm. A kinematical distance of 4 ± 0.5 kpc is found for G141.2+5.0, similar to the optical distance of the Cygnus arm (3.8 ± 1.1 kpc). G141.2+5.0 represents the first radio PWN discovered in 17 years and the first SNR discovered in the Cygnus spiral arm, which is in stark contrast with the Perseus arm's overwhelming population of shell-type remnants.


    Energy Technology Data Exchange (ETDEWEB)

    Acero, F.; Brandt, T. J. [NASA Goddard Space Flight Center, Greenbelt, MD 20771 (United States); Ackermann, M. [Deutsches Elektronen Synchrotron DESY, D-15738 Zeuthen (Germany); Ajello, M. [Space Sciences Laboratory, University of California, 7 Gauss Way, Berkeley, CA 94720-7450 (United States); Allafort, A.; Bechtol, K.; Blandford, R. D.; Bloom, E. D.; Bottacini, E.; Buehler, R. [W. W. Hansen Experimental Physics Laboratory, Kavli Institute for Particle Astrophysics and Cosmology, Department of Physics and SLAC National Accelerator Laboratory, Stanford University, Stanford, CA 94305 (United States); Baldini, L. [Universita di Pisa and Istituto Nazionale di Fisica Nucleare, Sezione di Pisa, I-56127 Pisa (Italy); Ballet, J. [Laboratoire AIM, CEA-IRFU/CNRS/Universite Paris Diderot, Service d' Astrophysique, CEA Saclay, F-91191 Gif sur Yvette (France); Barbiellini, G. [Istituto Nazionale di Fisica Nucleare, Sezione di Trieste, I-34127 Trieste (Italy); Bastieri, D.; Buson, S. [Istituto Nazionale di Fisica Nucleare, Sezione di Padova, I-35131 Padova (Italy); Bellazzini, R.; Bregeon, J. [Istituto Nazionale di Fisica Nucleare, Sezione di Pisa, I-56127 Pisa (Italy); Bonamente, E. [Istituto Nazionale di Fisica Nucleare, Sezione di Perugia, I-06123 Perugia (Italy); Brigida, M. [Dipartimento di Fisica ' ' M. Merlin' ' dell' Universita e del Politecnico di Bari, I-70126 Bari (Italy); Bruel, P., E-mail:, E-mail:, E-mail:, E-mail: [Laboratoire Leprince-Ringuet, Ecole polytechnique, CNRS/IN2P3, F-91128 Palaiseau (France); and others


    Pulsar wind nebulae (PWNe) have been established as the most populous class of TeV {gamma}-ray emitters. Since launch, the Fermi Large Area Telescope (LAT) has identified five high-energy (100 MeV < E < 100 GeV) {gamma}-ray sources as PWNe and detected a large number of PWN candidates, all powered by young and energetic pulsars. The wealth of multi-wavelength data available and the new results provided by Fermi-LAT give us an opportunity to find new PWNe and to explore the radiative processes taking place in known ones. The TeV {gamma}-ray unidentified (UNID) sources are the best candidates for finding new PWNe. Using 45 months of Fermi-LAT data for energies above 10 GeV, an analysis was performed near the position of 58 TeV PWNe and UNIDs within 5 Degree-Sign of the Galactic plane to establish new constraints on PWN properties and find new clues on the nature of UNIDs. Of the 58 sources, 30 were detected, and this work provides their {gamma}-ray fluxes for energies above 10 GeV. The spectral energy distributions and upper limits, in the multi-wavelength context, also provide new information on the source nature and can help distinguish between emission scenarios, i.e., between classification as a pulsar candidate or as a PWN candidate. Six new GeV PWN candidates are described in detail and compared with existing models. A population study of GeV PWN candidates as a function of the pulsar/PWN system characteristics is presented.


    Energy Technology Data Exchange (ETDEWEB)

    Ma, Y. K.; Ng, C.-Y. [Department of Physics, The University of Hong Kong, Pokfulam Road (Hong Kong); Bucciantini, N. [INAF—Osservatorio Astrofisico di Arcetri, L.go E. Fermi 5, I-50125 Firenze (Italy); Slane, P. O. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Gaensler, B. M. [Dunlap Institute for Astronomy and Astrophysics, The University of Toronto, Toronto, ON M5S 3H4 (Canada); Temim, T., E-mail: [Observational Cosmology Lab, Code 665, NASA Goddard Space Flight Center, Greenbelt, MD 20771 (United States)


    Pulsar wind nebulae (PWNe) are suggested to be acceleration sites of cosmic rays in the Galaxy. While the magnetic field plays an important role in the acceleration process, previous observations of magnetic field configurations of PWNe are rare, particularly for evolved systems. We present a radio polarization study of the “Snail” PWN inside the supernova remnant G327.1−1.1 using the Australia Telescope Compact Array. This PWN is believed to have been recently crushed by the supernova (SN) reverse shock. The radio morphology is composed of a main circular body with a finger-like protrusion. We detected a strong linear polarization signal from the emission, which reflects a highly ordered magnetic field in the PWN and is in contrast to the turbulent environment with a tangled magnetic field generally expected from hydrodynamical simulations. This could suggest that the characteristic turbulence scale is larger than the radio beam size. We built a toy model to explore this possibility, and found that a simulated PWN with a turbulence scale of about one-eighth to one-sixth of the nebula radius and a pulsar wind filling factor of 50%–75% provides the best match to observations. This implies substantial mixing between the SN ejecta and pulsar wind material in this system.

  2. Model for the broadband Crab nebula spectrum with injection of a log-parabola electron distribution at the wind termination shock (United States)

    Fraschetti, F.; Pohl, M.


    We develop a model of the steady-state spectrum of the Crab nebula encompassing both the radio/soft X-ray and the GeV/multi-TeV observations. By solving the transport equation for TeV electrons injected at the wind termination shock as a log-parabola momentum distribution and evolved via energy losses, we determine analytically the resulting photon differential energy spectrum. We find an impressive agreement with the observations in the synchrotron region. The predicted synchrotron self-Compton accommodates the previously unsolved origin of the broad 200 GeV peak that matches the Fermi/LAT data beyond 1 GeV with the MAGIC data. A natural interpretation of the deviation from power-law of the photon spectrum customarily fit with empirical broken power-laws is provided. This model can be applied to the radio-to- multi-TeV spectra of a variety of astrophysical outflows, including pulsar wind nebulae and supernova remnants. We also show that MeV-range energetic particle distribution at interplanetary shocks typically fit with broken-power laws or Band function can be accurately reproduced by log-parabolas.

  3. Synthesis of cyclic alpha-MSH peptides

    NARCIS (Netherlands)

    Schaaper, W.M.M.; Adan, R.A.H.; Posthuma, T.A.; Oosterom, J.; Gispen, W.H.; Meloen, R.H.


    Cyclic lactam analogs of α-melanocyte stimulating hormone (α-MSH) have been shown to be potent agonists in the frog skin bioassay [Al-Obeidi, F. et al., J. Med. Chem., 32 (1989) 2555], demonstrating melanocortin-1 (MC1) receptor activity. We synthesized cyclic α-MSH(1-13) and α-MSH(4-10) lactam

  4. Synthesis of cyclic α-MSH peptides

    NARCIS (Netherlands)

    Gispen, W.H.; Schaaper, W.M.M..; Adan, R.A.H.; Posthuma, T.A.; Oosterom, J.; Meloen, R.H.


    Cyclic lactam analogs of -melanocyte stimulating hormone (-MSH) have been shown to be potent agonists in the frog skin bioassay [Al-Obeidi, F. et al., J. Med. Chem., 32 (1989) 2555], demonstrating melanocortin-1 (MC1) receptor activity. We synthesized cyclic -MSH(1-13) and -MSH(4-10) lactam analogs.

  5. Time-dependent Electron Acceleration in Pulsar Wind Termination Shocks: Application to the 2011 April Crab Nebula Gamma-Ray Flare (United States)

    Kroon, John J.; Becker, Peter A.; Finke, Justin D.


    The γ-ray flares from the Crab Nebula observed by AGILE and Fermi-LAT between 2007 and 2013 reached GeV photon energies and lasted several days. The strongest emission, observed during the 2011 April “superflare”, exceeded the quiescent level by more than an order of magnitude. These observations challenge the standard models for particle acceleration in pulsar wind nebulae, because the radiating electrons have energies exceeding the classical radiation-reaction limit for synchrotron emission. Particle-in-cell simulations have suggested that the classical synchrotron limit can be exceeded if the electrons also experience electrostatic acceleration due to shock-driven magnetic reconnection. In this paper, we revisit the problem using an analytic approach based on solving a fully time-dependent electron transport equation describing the electrostatic acceleration, synchrotron losses, and escape experienced by electrons in a magnetically confined plasma “blob” as it encounters and passes through the pulsar wind termination shock. We show that our model can reproduce the γ-ray spectra observed during the rising and decaying phases of each of the two sub-flare components of the 2011 April superflare. We integrate the spectrum for photon energies ≥slant 100 MeV to obtain the light curve for the event, which also agrees with the observations. We find that strong electrostatic acceleration occurs on both sides of the termination shock, driven by magnetic reconnection. We also find that the dominant mode of particle escape changes from diffusive escape to advective escape as the blob passes through the shock.

  6. Observing nebulae

    CERN Document Server

    Griffiths, Martin


    This book enables anyone with suitable instruments to undertake an examination of nebulae and see or photograph them in detail. Nebulae, ethereal clouds of gas and dust, are among the most beautiful objects to view in the night sky. These star-forming regions are a common target for observers and photographers. Griffiths describes many of the brightest and best nebulae and includes some challenges for the more experienced observer. Readers learn the many interesting astrophysical properties of these clouds, which are an important subject of study in astronomy and astrobiology. Non-mathematical in approach, the text is easily accessible to anyone with an interest in the subject. A special feature is the inclusion of an observational guide to 70 objects personally observed or imaged by the author. The guide also includes photographs of each object for ease of identification along with their celestial coordinates, magnitudes and other pertinent information. Observing Nebulae provides a ready resource to allow an...

  7. X-Ray Analysis of the Proper Motion and Pulsar Wind Nebula for PSR J1741-2054 (United States)

    Auchettl, Katie; Slane, Patrick; Romani, Roger W.; Posselt, Bettina; Pavlov, George G.; Kargaltsev, Oleg; Ng, C-Y.; Temim, Tea; Weisskopf, Martin C.; Bykov, Andrei; hide


    We obtained six observations of PSR J1741-2054 using the Chandra ACIS-S detector totaling approx.300 ks. By registering this new epoch of observations to an archival observation taken 3.2 yr earlier using X-ray point sources in the field of view, we have measured the pulsar proper motion at micron = 109 +/- 10 mas yr(exp. -1) in a direction consistent with the symmetry axis of the observed H(alpha) nebula. We investigated the inferred past trajectory of the pulsar but find no compelling association with OB associations in which the progenitor may have originated. We confirm previous measurements of the pulsar spectrum as an absorbed power law with photon index gamma = 2.68 +/- 0.04, plus a blackbody with an emission radius of (4.5(+3.2/-2.5))d(0.38) km, for a DM-estimated distance of 0.38d(0.38) kpc and a temperature of 61.7 +/- 3.0 eV. Emission from the compact nebula is well described by an absorbed power law model with a photon index of gamma = 1.67 +/- 0.06, while the diffuse emission seen as a trail extending northeast of the pulsar shows no evidence of synchrotron cooling. We also applied image deconvolution techniques to search for small-scale structures in the immediate vicinity of the pulsar, but found no conclusive evidence for such structures.

  8. Physical structure of the fast and ultra-fast inner winds in the pre-planetary nebula He3-1475 (United States)

    Sanchez Contreras, C.; Sahai, R.


    Astronomers have long been puzzled by the morphological variety and bizzare shapes of planetary and pre-planetary nebulae (PNe and PPNe). Nowadays, an increasing number of astronomers believe that fast jet-like outflows ejected in the PPN phase are responsible for carving out the diverse shapes in the dense envelopes of their progenitor AGB stars. However, finding direct observational evidence for these elusive post-AGB jets is very difficult since they are thought to be compact, tenuous, and to operate only for a very short period of time (less than 1000yr). Also, a main difficulty in observing the jets directly is the presence of the dense ambient circumstellar material with which they interact. Recently, HST/STIS observations have allowed for the first time the direct study of the elusive post-AGB winds in one PPN, He3-1475 (Sanchez Contreras & Sahai 2001). Those winds have been discovered through blue-shifted absorption features in the Balmer and other recombination lines arising from the inner post-AGB jets and, ultimately, to understand their origin. Support for Proposal number AR-09554.03 was provided by NASA through a grant from the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Incorporated, under NASA contract NAS5-26555.

  9. Numerical nebulae

    NARCIS (Netherlands)

    Rijkhorst, Erik-Jan


    The late stages of evolution of stars like our Sun are dominated by several episodes of violent mass loss. Space based observations of the resulting objects, known as Planetary Nebulae, show a bewildering array of highly symmetric shapes. The interplay between gasdynamics and radiative processes

  10. Implications of the pulsar wind nebula scenario for a TeV gamma-ray source VER J2016+371 (United States)

    Saha, Lab


    We present multiwavelength studies of a TeV gamma-ray source VER J2016+371 suggested to be associated with a supernova remnant CTB 87 (G74.9+1.2) and based on X-ray and radio morphologies, CTB 87 is identified as an evolved pulsar wind nebula. A source in the vicinity of VER J2016+371 is also detected at GeV energies by Fermi Gamma Ray Space Telescope suggesting a likely counterpart at GeV energies. We find that a broken power-law (BPL) distribution of electrons can explain the observed data at radio, X-ray and TeV energies, however, is not sufficient to explain the data at MeV-GeV energies. A Maxwellian distribution of electrons along with the BPL distribution of electrons in low magnetic fields can explain the observed multiwavelength data spanned from radio to TeV energies suggesting this as the most likely scenario for this source. We also find that although the hadronic model can explain the observed GeV-TeV data for the ambient matter density of ˜ 20 cm- 3, no observational support for such high ambient density makes this hadronic scenario unlikely for this source.

  11. High-Energy X-rays from J174545.5-285829, the Cannonball: a Candidate Pulsar Wind Nebula Associated with Sgr a East (United States)

    Nynka, Melania; Hailey, Charles J.; Mori, Kaya; Baganoff, Frederick K.; Bauer, Franz E.; Boggs, Steven E.; Craig, William W.; Christensen, Finn E.; Gotthelf, Eric V.; Harrison, Fiona A.; hide


    We report the unambiguous detection of non-thermal X-ray emission up to 30 keV from the Cannonball, a few arcsecond long diffuse X-ray feature near the Galactic Center, using the NuSTAR X-ray observatory. The Cannonball is a high-velocity (v(proj) approximately 500 km s(exp -1)) pulsar candidate with a cometary pulsar wind nebula (PWN) located approximately 2' north-east from Sgr A*, just outside the radio shell of the supernova remnant Sagittarius A (Sgr A) East. Its non-thermal X-ray spectrum, measured up to 30 keV, is well characterized by a Gamma is approximately 1.6 power law, typical of a PWN, and has an X-ray luminosity of L(3-30 keV) = 1.3 × 10(exp 34) erg s(exp -1). The spectral and spatial results derived from X-ray and radio data strongly suggest a runaway neutron star born in the Sgr A East supernova event. We do not find any pulsed signal from the Cannonball. The NuSTAR observations allow us to deduce the PWN magnetic field and show that it is consistent with the lower limit obtained from radio observations.

  12. Late-Time Evolution of Composite Supernova Remnants: Deep Chandra Observations and Hydrodynamical Modeling of a Crushed Pulsar Wind Nebula in SNR G327.1-1.1 (United States)

    Temim, Tea; Slane, Patrick; Kolb, Christopher; Blondin, John; Hughes, John P.; Bucciantini, Niccolo


    In an effort to better understand the evolution of composite supernova remnants (SNRs) and the eventual fate of relativistic particles injected by their pulsars, we present a multifaceted investigation of the interaction between a pulsar wind nebula (PWN) and its host SNR G327.1-1.1. Our 350 ks Chandra X-ray observations of SNR G327.1-1.1 reveal a highly complex morphology; a cometary structure resembling a bow shock, prong-like features extending into large arcs in the SNR interior, and thermal emission from the SNR shell. Spectral analysis of the non-thermal emission offers clues about the origin of the PWN structures, while enhanced abundances in the PWN region provide evidence for mixing of supernova ejecta with PWN material. The overall morphology and spectral properties of the SNR suggest that the PWN has undergone an asymmetric interaction with the SNR reverse shock(RS) that can occur as a result of a density gradient in the ambient medium and or a moving pulsar that displaces the PWN from the center of the remnant. We present hydrodynamical simulations of G327.1-1.1 that show that its morphology and evolution can be described by a approx. 17,000 yr old composite SNR that expanded into a density gradient with an orientation perpendicular to the pulsar's motion. We also show that the RSPWN interaction scenario can reproduce the broadband spectrum of the PWN from radio to gamma-ray wavelengths. The analysis and modeling presented in this work have important implications for our general understanding of the structure and evolution of composite SNRs.


    Energy Technology Data Exchange (ETDEWEB)

    Temim, Tea [Observational Cosmology Lab, Code 665, NASA Goddard Space Flight Center, Greenbelt, MD 20771 (United States); Slane, Patrick [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Kolb, Christopher; Blondin, John [North Carolina State University, 421 Riddick Hall, Raleigh, NC 27695 (United States); Hughes, John P. [Rutgers University, 57 US Highway 1, New Brunswick, NJ 08901 (United States); Bucciantini, Niccoló [INAF Osservatorio Astrofisico di Arcetri, Largo Enrico Fermi, 5, 50125, Firenze Italy (Italy)


    In an effort to better understand the evolution of composite supernova remnants (SNRs) and the eventual fate of relativistic particles injected by their pulsars, we present a multifaceted investigation of the interaction between a pulsar wind nebula (PWN) and its host SNR G327.1-1.1. Our 350 Chandra X-ray observations of SNR G327.1-1.1 reveal a highly complex morphology: a cometary structure resembling a bow shock, prong-like features extending into large arcs in the SNR interior, and thermal emission from the SNR shell. Spectral analysis of the non-thermal emission offers clues about the origin of the PWN structures, while enhanced abundances in the PWN region provide evidence for a mixing of supernova ejecta with PWN material. The overall morphology and spectral properties of the SNR suggest that the PWN has undergone an asymmetric interaction with the SNR reverse shock (RS), whichcan occur as a result of a density gradient in the ambient medium and/or a moving pulsar that displaces the PWN from the center of the remnant. We present hydrodynamical simulations of G327.1-1.1 that show that its morphology and evolution can be described by a ∼17,000-year-old composite SNR that expanded into a density gradient with an orientation perpendicular to the pulsar’s motion. We also show that the RS/PWN interaction scenario can reproduce the broadband spectrum of the PWN from radio to γ-ray wavelengths. The analysis and modeling presented in this work have important implications for our general understanding of the structure and evolution of composite SNRs.


    Energy Technology Data Exchange (ETDEWEB)

    Murase, Kohta [Institute for Advanced Study, Princeton, NJ 08540 (United States); Kashiyama, Kazumi [Center for Particle and Gravitational Astrophysics, Department of Physics, Department of Astronomy and Astrophysics, Pennsylvania State University, University Park, PA 16802 (United States); Kiuchi, Kenta [Yukawa Institute for Theoretical Physics, Kyoto University, Kyoto, Kyoto 606-8502 (Japan); Bartos, Imre [Department of Physics, Columbia Astrophysics Laboratory, Columbia University, New York, NY 10027 (United States)


    It has been suggested that some classes of luminous supernovae (SNe) and gamma-ray bursts (GRBs) are driven by newborn magnetars. Fast-rotating proto-neutron stars have also been of interest as potential sources of gravitational waves (GWs). We show that for a range of rotation periods and magnetic fields, hard X-rays and GeV gamma rays provide us with a promising probe of pulsar-aided SNe. It is observationally known that young pulsar wind nebulae (PWNe) in the Milky Way are very efficient lepton accelerators. We argue that, if embryonic PWNe satisfy similar conditions at early stages of SNe (in ∼1–10 months after the explosion), external inverse-Compton emission via upscatterings of SN photons is naturally expected in the GeV range as well as broadband synchrotron emission. To fully take into account the Klein–Nishina effect and two-photon annihilation process that are important at early times, we perform detailed calculations including electromagnetic cascades. Our results suggest that hard X-ray telescopes such as NuSTAR can observe such early PWN emission by follow-up observations in months to years. GeV gamma-rays may also be detected by Fermi for nearby SNe, which serve as counterparts of these GW sources. Detecting the signals will give us an interesting probe of particle acceleration at early times of PWNe, as well as clues to driving mechanisms of luminous SNe and GRBs. Since the Bethe–Heitler cross section is lower than the Thomson cross section, gamma rays would allow us to study subphotospheric dissipation. We encourage searches for high-energy emission from nearby SNe, especially SNe Ibc including super-luminous objects.

  15. The predicted truncation from a cancer-associated variant of the MSH2 initiation codon alters activity of the MSH2-MSH6 mismatch repair complex. (United States)

    Cyr, Jennifer L; Brown, Graham D; Stroop, Jennifer; Heinen, Christopher D


    Lynch syndrome (LS) is caused by germline mutations in DNA mismatch repair (MMR) genes. MMR recognizes and repairs DNA mismatches and small insertion/deletion loops. Carriers of MMR gene variants have a high risk of developing colorectal, endometrial, ovarian, and other extracolonic carcinomas. We report on an ovarian cancer patient who carries a germline MSH2 c.1A>C variant which alters the translation initiation codon. Mutations affecting the MSH2 start codon have been described previously for LS-related malignancies. However, the patients often lack a clear family history indicative of LS and their tumors often fail to display microsatellite instability, a hallmark feature of LS. Therefore, the pathogenicity of start codon variants remains undefined. Loss of the MSH2 start codon has been predicted to result in a truncated protein translated from a downstream in-frame AUG that would lack the first 25 amino acids. We therefore purified recombinant MSH2(NΔ25)-MSH6 and MSH2(NΔ25)-MSH3 to examine their DNA lesion recognition and adenosine nucleotide processing functions in vitro. We found that the MSH2(NΔ25) mutant confers distinct biochemical defects on MSH2-MSH6, but does not have a significant effect on MSH2-MSH3. We confirmed that expression of the MSH2 c.1A>C cDNA results in the production of multiple protein products in human cells that may include the truncated and full-length forms of MSH2. An in vivo MMR assay revealed a slight reduction in MMR efficiency in these cells. These data suggest that mutation of the MSH2 initiation codon, while not a strong, high-risk disease allele, may have a moderate impact on disease phenotype. Copyright © 2011 Wiley Periodicals, Inc.

  16. A rare MSH2 mutation causes defective binding to hMSH6, normal hMSH2 staining, and loss of hMSH6 at advanced cancer stage. (United States)

    Loconte, Daria Carmela; Patruno, Margherita; Lastella, Patrizia; Di Gregorio, Carmela; Grossi, Valentina; Forte, Giovanna; Ingravallo, Giuseppe; Varvara, Dora; Bagnulo, Rosanna; Simone, Cristiano; Resta, Nicoletta; Stella, Alessandro


    Lynch syndrome is caused by germline mutations in 1 of the 4 DNA mismatch repair genes (MLH1, MSH2, MSH6, and PMS2). Mutations in MSH2 cause concomitant loss of hMSH6, whereas MLH1 mutations lead to concurrent loss of PMS2. Much less frequent mutations in MSH6 or PMS2 are associated with the isolated loss of the corresponding proteins. We here demonstrate the causative role of the first germline mutation of MSH2, c.1249-1251 dupGTT (p.417V-418I dupV), associated with normal hMSH2 expression and lack of hMSH6 protein despite a normal MSH6 gene sequence. hMSH6 protein was completely lost only in advanced cancer stages due to 2 different "second hits": a whole MSH2 gene deletion and a frame-shifting insertion in the MSH6 (C)8 repeat in the coding sequence. Copyright © 2014 Elsevier Inc. All rights reserved.

  17. Where Do Messy Planetary Nebulae Come From? (United States)

    Kohler, Susanna


    If you examined images of planetary nebulae, you would find that many of them have an appearance that is too messy to be accounted for in the standard model of how planetary nebulae form. So what causes these structures?Examples of planetary nebulae that have a low probability of having beenshaped by a triple stellar system. They are mostly symmetric, with only slight departures (labeled) that can be explained by instabilities, interactions with the interstellar medium, etc. [Bear and Soker 2017]A Range of LooksAt the end of a stars lifetime, in the red-giant phase, strong stellar winds can expel the outer layers of the star. The hot, luminous core then radiates in ultraviolet, ionizing the gas of the ejected stellar layers and causing them to shine as a brightly colored planetary nebula for a few tens of thousands of years.Planetary nebulae come in a wide variety of morphologies. Some are approximately spherical, but others can be elliptical, bipolar, quadrupolar, or even more complex.Its been suggested that non-spherical planetary nebulae might be shaped by the presence of a second star in a binary system with the source of the nebula but even this scenario should still produce a structure with axial or mirror symmetry.A pair of scientists from Technion Israel Institute of Technology, Ealeal Bear and Noam Soker, argue that planetary nebulae with especially messy morphologies those without clear axial or point symmetries may have been shaped by an interacting triple stellar system instead.Examples of planetary nebulae that might have been shaped by a triple stellar system. They have some deviations from symmetry but also show signs of interacting with the interstellar medium. [Bear and Soker 2017]Departures from SymmetryTo examine this possibility more closely, Bear and Soker look at a sample of thousands planetary nebulae and qualitatively classify each of them into one of four categories, based on the degree to which they show signs of having been shaped by a

  18. ATP binding and hydrolysis by Saccharomyces cerevisiae Msh2-Msh3 are differentially modulated by mismatch and double-strand break repair DNA substrates. (United States)

    Kumar, Charanya; Eichmiller, Robin; Wang, Bangchen; Williams, Gregory M; Bianco, Piero R; Surtees, Jennifer A


    In Saccharomyces cerevisiae, Msh2-Msh3-mediated mismatch repair (MMR) recognizes and targets insertion/deletion loops for repair. Msh2-Msh3 is also required for 3' non-homologous tail removal (3'NHTR) in double-strand break repair. In both pathways, Msh2-Msh3 binds double-strand/single-strand junctions and initiates repair in an ATP-dependent manner. However, we recently demonstrated that the two pathways have distinct requirements with respect to Msh2-Msh3 activities. We identified a set of aromatic residues in the nucleotide binding pocket (FLY motif) of Msh3 that, when mutated, disrupted MMR, but left 3'NHTR largely intact. One of these mutations, msh3Y942A, was predicted to disrupt the nucleotide sandwich and allow altered positioning of ATP within the pocket. To develop a mechanistic understanding of the differential requirements for ATP binding and/or hydrolysis in the two pathways, we characterized Msh2-Msh3 and Msh2-msh3Y942A ATP binding and hydrolysis activities in the presence of MMR and 3'NHTR DNA substrates. We observed distinct, substrate-dependent ATP hydrolysis and nucleotide turnover by Msh2-Msh3, indicating that the MMR and 3'NHTR DNA substrates differentially modify the ATP binding/hydrolysis activities of Msh2-Msh3. Msh2-msh3Y942A retained the ability to bind DNA and ATP but exhibited altered ATP hydrolysis and nucleotide turnover. We propose that both ATP and structure-specific repair substrates cooperate to direct Msh2-Msh3-mediated repair and suggest an explanation for the msh3Y942A separation-of-function phenotype. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. ATP binding and hydrolysis by Saccharomyces cerevisiae Msh2-Msh3 are differentially modulated by Mismatch and Double-strand Break Repair DNA substrates (United States)

    Kumar, Charanya; Eichmiller, Robin; Wang, Bangchen; Williams, Gregory M.; Bianco, Piero R.; Surtees, Jennifer A.


    In Saccharomyces cerevisiae, Msh2-Msh3-mediated mismatch repair (MMR) recognizes and targets insertion/deletion loops for repair. Msh2-Msh3 is also required for 3′ non-homologous tail removal (3′NHTR) in double-strand break repair. In both pathways, Msh2-Msh3 binds double-strand/single-strand junctions and initiates repair in an ATP-dependent manner. However, we recently demonstrated that the two pathways have distinct requirements with respect to Msh2-Msh3 activities. We identified a set of aromatic residues in the nucleotide binding pocket (FLY motif) of Msh3 that, when mutated, disrupted MMR, but left 3′ NHTR largely intact. One of these mutations, msh3Y942A, was predicted to disrupt the nucleotide sandwich and allow altered positioning of ATP within the pocket. To develop a mechanistic understanding of the differential requirements for ATP binding and/or hydrolysis in the two pathways, we characterized Msh2-Msh3 and Msh2-msh3Y942A ATP binding and hydrolysis activities in the presence of MMR and 3′ NHTR DNA substrates. We observed distinct, substrate-dependent ATP hydrolysis and nucleotide turnover by Msh2-Msh3, indicating that the MMR and 3′ NHTR DNA substrates differentially modify the ATP binding/hydrolysis activities of Msh2-Msh3. Msh2-msh3Y942A retained the ability to bind DNA and ATP but exhibited altered ATP hydrolysis and nucleotide turnover. We propose that both ATP and structure-specific repair substrates cooperate to direct Msh2-Msh3-mediated repair and suggest an explanation for the msh3Y942A separation-of-function phenotype. PMID:24746922

  20. Functional examination of MLH1, MSH2, and MSH6 intronic mutations identified in Danish colorectal cancer patients

    DEFF Research Database (Denmark)

    Petersen, S. M.; Dandanell, M.; Rasmussen, L. J.


    to asses the effect on splicing. Results: We describe in silico and in vitro characterization of nine intronic MLH1, MSH2, or MSH6 mutations identified in Danish colorectal cancer patients, of which four mutations are novel. The analysis revealed aberrant splicing of five mutations (MLH1 c. 588 + 5G > A......Background: Germ-line mutations in the DNA mismatch repair genes MLH1, MSH2, and MSH6 predispose to the development of colorectal cancer (Lynch syndrome or hereditary nonpolyposis colorectal cancer). These mutations include disease-causing frame-shift, nonsense, and splicing mutations as well...... as large genomic rearrangements. However, a large number of mutations, including missense, silent, and intronic variants, are classified as variants of unknown clinical significance. Methods: Intronic MLH1, MSH2, or MSH6 variants were investigated using in silico prediction tools and mini-gene assay...

  1. A SOFIA FORCAST Grism Study of the Mineralogy of Dust in the Winds of Proto-planetary Nebulae: RV Tauri Stars and SRd Variables (United States)

    Arneson, R. A.; Gehrz, R. D.; Woodward, C. E.; Helton, L. A.; Shenoy, D.; Evans, A.; Keller, L. D.; Hinkle, K. H.; Jura, M.; Lebzelter, T.; Lisse, C. M.; Rushton, M. T.; Mizrachi, J.


    We present a SOFIA FORCAST grism spectroscopic survey to examine the mineralogy of the circumstellar dust in a sample of post-asymptotic giant branch (post-AGB) yellow supergiants that are believed to be the precursors of planetary nebulae. Our mineralogical model of each star indicates the presence of both carbon-rich and oxygen-rich dust species—contrary to simple dredge-up models—with a majority of the dust in the form of amorphous carbon and graphite. The oxygen-rich dust is primarily in the form of amorphous silicates. The spectra do not exhibit any prominent crystalline silicate emission features. For most of the systems, our analysis suggests that the grains are relatively large and have undergone significant processing, supporting the hypothesis that the dust is confined to a Keplerian disk and that we are viewing the heavily processed, central regions of the disk from a nearly face-on orientation. These results help to determine the physical properties of the post-AGB circumstellar environment and to constrain models of post-AGB mass loss and planetary nebula formation.

  2. Association of rare MSH6 variants with familial breast cancer

    NARCIS (Netherlands)

    Wasielewski, Marijke; Riaz, Muhammad; Vermeulen, Joyce; van den Ouweland, Ans; Labrijn-Marks, Ineke; Olmer, Renske; van der Spaa, Linda; Klijn, Jan G. M.; Meijers-Heijboer, Hanne; Dooijes, Dennis; Schutte, Mieke


    Germline mutations in the mismatch repair genes MLH1, MSH2, MSH6, and PMS2 predispose to Lynch syndrome (also known as hereditary non-polyposis colorectal cancer). Recently, we have shown that the CHEK2 1100delC mutation also is associated with Lynch syndrome/Lynch syndrome-associated families

  3. Saturn Nebula (NGC 7009) (United States)

    Murdin, P.


    A planetary nebula in the constellation Aquarius, position RA 21 h 04.4 m, dec. -11° 22'. It measures 25'' and shines with a greenish hue. It is of eighth magnitude and for a planetary nebula has a high surface brightness. Two small lobes on either side give it the appearance of the planet Saturn....



    [Top left] - IC 3568 lies in the constellation Camelopardalis at a distance of about 9,000 light-years, and has a diameter of about 0.4 light-years (or about 800 times the diameter of our solar system). It is an example of a round planetary nebula. Note the bright inner shell and fainter, smooth, circular outer envelope. Credits: Howard Bond (Space Telescope Science Institute), Robin Ciardullo (Pennsylvania State University) and NASA [Top center] - NGC 6826's eye-like appearance is marred by two sets of blood-red 'fliers' that lie horizontally across the image. The surrounding faint green 'white' of the eye is believed to be gas that made up almost half of the star's mass for most of its life. The hot remnant star (in the center of the green oval) drives a fast wind into older material, forming a hot interior bubble which pushes the older gas ahead of it to form a bright rim. (The star is one of the brightest stars in any planetary.) NGC 6826 is 2,200 light- years away in the constellation Cygnus. The Hubble telescope observation was taken Jan. 27, 1996 with the Wide Field and Planetary Camera 2. Credits: Bruce Balick (University of Washington), Jason Alexander (University of Washington), Arsen Hajian (U.S. Naval Observatory), Yervant Terzian (Cornell University), Mario Perinotto (University of Florence, Italy), Patrizio Patriarchi (Arcetri Observatory, Italy) and NASA [Top right ] - NGC 3918 is in the constellation Centaurus and is about 3,000 light-years from us. Its diameter is about 0.3 light-year. It shows a roughly spherical outer envelope but an elongated inner balloon inflated by a fast wind from the hot central star, which is starting to break out of the spherical envelope at the top and bottom of the image. Credits: Howard Bond (Space Telescope Science Institute), Robin Ciardullo (Pennsylvania State University) and NASA [Bottom left] - Hubble 5 is a striking example of a 'butterfly' or bipolar (two-lobed) nebula. The heat generated by fast winds causes

  5. The predicted truncation from a cancer-associated variant of the MSH2 initiation codon alters activity of the MSH2-MSH6 mismatch repair complex


    Cyr, Jennifer L.; Brown, Graham D; Stroop, Jennifer; Heinen, Christopher D.


    Lynch syndrome (LS) is caused by germline mutations in DNA mismatch repair (MMR) genes. M M R recognizes and repairs DNA mismatches and small insertion/deletion loops. Carriers of MMR gene variants have a high risk of developing colorectal, endometrial, ovarian, and other extracolonic carcinomas. We report on an ovarian cancer patient who carries a germline MSH2 c.1A>C variant which alters the translation initiation codon. Mutations affecting the MSH2 start codon have been described previousl...

  6. A Shocking Solar Nebula?


    Liffman, Kurt


    It has been suggested that shock waves in the solar nebula formed the high temperature materials observed in meteorites and comets. It is shown that the temperatures at the inner rim of the solar nebula could have been high enough over a sufficient length of time to produce chondrules, CAIs, refractory dust grains and other high-temperature materials observed in comets and meteorites. The solar bipolar jet flow may have produced an enrichment of 16O in the solar nebula over time and the chond...

  7. Helix Nebula (NGC 7293) (United States)

    Murdin, P.


    A planetary nebula in the constellation Aquarius, position RA 22 h 29.6 m, dec. -20° 48'. It is the nearest planetary nebula to Earth, it has the largest angular size, at 15' by 12', and its magnitude of 6.5 makes it the brightest (though like all extended objects it does not have a high surface brightness). It is illuminated by its hot thirteenth-magnitude central star. The Helix gets its name f...

  8. The evolution of planetary nebulae. VII. Modelling planetary nebulae of distant stellar systems (United States)

    Schönberner, D.; Jacob, R.; Sandin, C.; Steffen, M.


    Aims: By means of hydrodynamical models we do the first investigations of how the properties of planetary nebulae are affected by their metal content and what can be learned from spatially unresolved spectrograms of planetary nebulae in distant stellar systems. Methods: We computed a new series of 1D radiation-hydrodynamics planetary nebulae model sequences with central stars of 0.595 M⊙ surrounded by initial envelope structures that differ only by their metal content. At selected phases along the evolutionary path, the hydrodynamic terms were switched off, allowing the models to relax for fixed radial structure and radiation field into their equilibrium state with respect to energy and ionisation. The analyses of the line spectra emitted from both the dynamical and static models enabled us to systematically study the influence of hydrodynamics as a function of metallicity and evolution. We also recomputed selected sequences already used in previous publications, but now with different metal abundances. These sequences were used to study the expansion properties of planetary nebulae close to the bright cut-off of the planetary nebula luminosity function. Results: Our simulations show that the metal content strongly influences the expansion of planetary nebulae: the lower the metal content, the weaker the pressure of the stellar wind bubble, but the faster the expansion of the outer shell because of the higher electron temperature. This is in variance with the predictions of the interacting-stellar-winds model (or its variants) according to which only the central-star wind is thought to be responsible for driving the expansion of a planetary nebula. Metal-poor objects around slowly evolving central stars become very dilute and are prone to depart from thermal equilibrium because then adiabatic expansion contributes to gas cooling. We find indications that photoheating and line cooling are not fully balanced in the evolved planetary nebulae of the Galactic halo

  9. Deciphering the Mismatch Recognition Cycle in MutS and MSH2-MSH6 Using Normal-Mode Analysis


    Mukherjee, Shayantani; Law, Sean M.; Feig, Michael


    Postreplication DNA mismatch repair is essential for maintaining the integrity of genomic information in prokaryotes and eukaryotes. The first step in mismatch repair is the recognition of base-base mismatches and insertions/deletions by bacterial MutS or eukaryotic MSH2-MSH6. Crystal structures of both proteins bound to mismatch DNA reveal a similar molecular architecture but provide limited insight into the detailed molecular mechanism of long-range allostery involved in mismatch recognitio...

  10. Deciphering the mismatch recognition cycle in MutS and MSH2-MSH6 using normal-mode analysis. (United States)

    Mukherjee, Shayantani; Law, Sean M; Feig, Michael


    Postreplication DNA mismatch repair is essential for maintaining the integrity of genomic information in prokaryotes and eukaryotes. The first step in mismatch repair is the recognition of base-base mismatches and insertions/deletions by bacterial MutS or eukaryotic MSH2-MSH6. Crystal structures of both proteins bound to mismatch DNA reveal a similar molecular architecture but provide limited insight into the detailed molecular mechanism of long-range allostery involved in mismatch recognition and repair initiation. This study describes normal-mode calculations of MutS and MSH2-MSH6 with and without DNA. The results reveal similar protein flexibilities and suggest common dynamic and functional characteristics. A strongly correlated motion is present between the lever domain and ATPase domains, which suggests a pathway for long-range allostery from the N-terminal DNA binding domain to the C-terminal ATPase domains, as indicated by experimental studies. A detailed analysis of individual low-frequency modes of both MutS and MSH2-MSH6 shows changes in the DNA-binding domains coupled to the ATPase sites, which are interpreted in the context of experimental data to arrive at a complete molecular-level mismatch recognition cycle. Distinct conformational states are proposed for DNA scanning, mismatch recognition, repair initiation, and sliding along DNA after mismatch recognition. Hypotheses based on the results presented here form the basis for further experimental and computational studies.

  11. Cool gaseous nebulae

    CERN Document Server

    Shaver, P A; Pottasch, S R


    The electron temperatures of diffuse gaseous nebulae have long been thought to be close to 10/sup 4/K. Much lower temperatures were derived from some of the early radio continuum and recombination line work, but these were generally considered to be wrong for a variety of reasons. While there is little doubt that the bright nebulae do indeed have temperatures of approximately 8000-9000K, there are strong indications that some nebulae of lower densities have much lower temperatures, nebulae were made in order to determine electron temperatures in the absence of such effects as collisional de-excitation, stimulated emission, and pressure broadening. Several of these nebulae have been found to have temperatures below 5000K and for two of them which are discussed (RCW94 and G339.1-0.2) absolute upper limits of approximately 4700 K are imposed by the line widths alone. (11 refs).

  12. Catalogues of planetary nebulae. (United States)

    Acker, A.

    Firstly, the general requirements concerning catalogues are studied for planetary nebulae, in particular concerning the objects to be included in a catalogue of PN, their denominations, followed by reflexions about the afterlife and comuterized versions of a catalogue. Then, the basic elements constituting a catalogue of PN are analyzed, and the available data are looked at each time.

  13. [Value of the MSH classification in the hemiplegic stroke patient]. (United States)

    Pinedo-Otaola, S; de la Villa, F M

    The functional prognosis of patients should be known when planning their rehabilitation treatment. To find the prognostic value of the MSH classification for hemiplegic patients, which is based on the clinical deficits seen after the stroke, and to distinguish between the M group (motor deficit), MS group (motor and sensory deficits) and the MSH group (motor, sensory and homonymous deficits). A prospective analytical study was made of the hemiplegic patients admitted consecutively to the Rehabilitation Department after strokes. The initial examination was made 17 days (CI: 15-19) after the stroke. Satisfactory functional results were considered to be obtaining a Barthel index of > 90 together with the ability to walk unaided on level ground six months after the stroke. Of the 55 patients finally included in the study, 23 were in group M (41.8%) 21 in group MS (38.1%) and 11 patients in group MSH (20.1%). Walking ability and function in the different cohorts showed a statistically significant difference. Whilst 96% of the patients with pure hemiparesia (group M) attained a satisfactory degree of independence in walking, only 54% of the patients with three associated deficits (MSH) achieved this. Even more difference was found with acquired functional independence, since whilst 82% of group M attained a good degree of autonomy in carrying out everyday activities, only 18% of the MSH group achieved this. The remaining patients (MS group) had intermediate results. The MSH classification is, soon after having a stroke, a useful means of determining the functional prognosis of hemiplegic patients.

  14. Planetary nebulae with UVIT: Far ultra-violet halo around the Bow Tie nebula (NGC 40) (United States)

    Kameswara Rao, N.; Sutaria, F.; Murthy, J.; Krishna, S.; Mohan, R.; Ray, A.


    Context. NGC 40 is a planetary nebula with diffuse X-ray emission, suggesting an interaction of the high-speed wind from WC8 central star (CS) with the nebula. It shows strong C IV 1550 Å emission that cannot be explained by thermal processes alone. We present here the first map of this nebula in C IV emission using broad band filters on the Ultra-Violet Imaging Telescope (UVIT). Aim. We aim to map the hot C IV-emitting gas and its correspondence with soft X-ray (0.3-8 keV) emitting regions in order to study the shock interaction between the nebula and the ISM. We also aim to illustrate the potential of UVIT for nebular studies. Methods: We carry out a morphological study of images of the nebula obtained at an angular resolution of about 1.3″ in four UVIT filter bands that include C IV 1550 Å and [C II] 2326 Å lines as well as UV continuum. We also make comparisons with X-ray, optical, and IR images from the literature. Results: The [C II] 2326 Å images show the core of the nebula with two lobes on either side of CS similar to [N II]. The C IV emission in the core shows similar morphology and extent to that of diffuse X-ray emission concentrated in nebular condensations. A surprising UVIT discovery is the presence of a large faint far UV (FUV) halo in an FUV filter with λeff of 1608 Å. The UV halo is not present in any other UV filter. The FUV halo is most likely due to UV fluorescence emission from the Lyman bands of H2 molecules. Unlike the optical and IR halo, the FUV halo trails predominantly towards the south-east side of the nebular core, opposite to the CS's proper motion direction. Conclusions: Morphological similarity of C IV 1550 Å and X-ray emission in the core suggests that it results mostly from the interaction of strong CS wind with the nebula. The FUV halo in NGC 40 highlights the extensive existence of H2 molecules in the regions even beyond the optical and IR halos. Thus UV studies are important to estimate the amount of H2, which is

  15. Human MSH6 deficiency is associated with impaired antibody maturation. (United States)

    Gardès, Pauline; Forveille, Monique; Alyanakian, Marie-Alexandra; Aucouturier, Pierre; Ilencikova, Denisa; Leroux, Dominique; Rahner, Nils; Mazerolles, Fabienne; Fischer, Alain; Kracker, Sven; Durandy, Anne


    Ig class-switch recombination (Ig-CSR) deficiencies are rare primary immunodeficiencies characterized by defective switched isotype (IgG/IgA/IgE) production. Depending on the molecular defect, defective Ig-CSR may also be associated with impaired somatic hypermutation (SHM) of the Ig V regions. Although the mechanisms underlying Ig-CSR and SHM in humans have been revealed (at least in part) by studying natural mutants, the role of mismatch repair in this process has not been fully elucidated. We studied in vivo and in vitro Ab maturation in eight MSH6-deficient patients. The skewed SHM pattern strongly suggests that MSH6 is involved in the human SHM process. Ig-CSR was found to be partially defective in vivo and markedly impaired in vitro. The resolution of γH2AX foci following irradiation of MSH6-deficient B cell lines was also found to be impaired. These data suggest that in human CSR, MSH6 is involved in both the induction and repair of DNA double-strand breaks in switch regions.

  16. Alpha-MSH decreases core and brain temperature during global cerebral ischemia in rats

    DEFF Research Database (Denmark)

    Spulber, S.; Moldovan, Mihai; Oprica, M.


    -vessel occlusion forebrain ischemia on core temperature (CT) and brain temperature (BT), respectively. After 10 min cerebral ischemia, BT was lower in alpha-MSH- than in saline-injected animals. After 10 min reperfusion, both CT and BT were lower than the corresponding pre-ischemic levels after injection of alpha......-MSH. alpha-MSH did not influence CT or BT in sham-operated rats. The alpha-MSH-induced hypothermia and its potentiation of reduction in BT during global cerebral ischemia, may contribute to neuroprotective effects of alpha-MSH....

  17. The Crab Nebula flaring activity

    Directory of Open Access Journals (Sweden)

    G. Montani


    Full Text Available The discovery made by AGILE and Fermi of a short time scale flaring activity in the gamma-ray energy emission of the Crab Nebula is a puzzling and unexpected feature, challenging particle acceleration theory. In the present work we propose the shock-induced magnetic reconnection as a viable mechanism to explain the Crab flares. We postulate that the emitting region is located at ∼1015 cm from the central pulsar, well inside the termination shock, which is exactly the emitting region size as estimated by the overall duration of the phenomenon ∼1 day. We find that this location corresponds to the radial distance at which the shock-induced magnetic reconnection process is able to accelerate the electrons up to a Lorentz factor ∼109, as required by the spectral fit of the observed Crab flare spectrum. The main merit of the present analysis is to highlight the relation between the observational constraints to the flare emission and the radius at which the reconnection can trigger the required Lorentz factor. We also discuss different scenarios that can induce the reconnection. We conclude that the existence of a plasma instability affecting the wind itself as the Weibel instability is the privileged scenario in our framework.

  18. Nine novel pathogenic germline mutations in MLH1, MSH2, MSH6 and PMS2 in families with Lynch syndrome. (United States)

    Rahner, Nils; Friedrichs, Nicolaus; Wehner, Maria; Steinke, Verena; Aretz, Stefan; Friedl, Waltraut; Buettner, Reinhard; Mangold, Elisabeth; Propping, Peter; Walldorf, Constanze


    Many germline mutations in the DNA mismatch repair genes have been described so far leading to the clinical phenotype of Lynch syndrome (hereditary nonpolyposis colorectal cancer, HNPCC). Most mutations are private mutations. We report on nine novel pathogenic germline mutations that have been found in families meeting either the Amsterdam or the Bethesda criteria. These findings include the mutations MLH1,c.884+4A>G, MLH1,c.1377_1378insA;p.Glu460ArgfsX19, MLH1,c.1415_1416delGA;p.Arg472ThrfsX5, MSH2,c.301G>T;p.Glu101X, MSH2,c.638_639delTG;p.Leu213GlnfsX18, MSH2,c.842C>A;p.Ser281X, MSH2,c.859G>T;p.Gly287X, MSH6,c.2503C>T;p.Gln835X and a large genomic deletion of exons 1-10 of the PMS2 gene. The mutation MLH1,c.884+4A>G detected in two families results in a complete skipping of exon 10 on mRNA level and thus has been considered as pathogenic. In all cases the tumor tissue of the index patient revealed high microsatellite instability (MSI-H) and showed a complete loss of expression of the affected protein in the tumor cells by immunohistochemistry (IHC). The findings underline the importance of a pre-screening of tumor tissue for an efficient definition of conspicuous cases.

  19. MutSβ abundance and Msh3 ATP hydrolysis activity are important drivers of CTG•CAG repeat expansions. (United States)

    Keogh, Norma; Chan, Kara Y; Li, Guo-Min; Lahue, Robert S


    CTG•CAG repeat expansions cause at least twelve inherited neurological diseases. Expansions require the presence, not the absence, of the mismatch repair protein MutSβ (Msh2-Msh3 heterodimer). To evaluate properties of MutSβ that drive expansions, previous studies have tested under-expression, ATPase function or polymorphic variants of Msh2 and Msh3, but in disparate experimental systems. Additionally, some variants destabilize MutSβ, potentially masking the effects of biochemical alterations of the variations. Here, human Msh3 was mutated to selectively inactivate MutSβ. Msh3-/- cells are severely defective for CTG•CAG repeat expansions but show full activity on contractions. Msh3-/- cells provide a single, isogenic system to add back Msh3 and test key biochemical features of MutSβ on expansions. Msh3 overexpression led to high expansion activity and elevated levels of MutSβ complex, indicating that MutSβ abundance drives expansions. An ATPase-defective Msh3 expressed at normal levels was as defective in expansions as Msh3-/- cells, indicating that Msh3 ATPase function is critical for expansions. Expression of two Msh3 polymorphic variants at normal levels showed no detectable change in expansions, suggesting these polymorphisms primarily affect Msh3 protein stability, not activity. In summary, CTG•CAG expansions are limited by the abundance of MutSβ and rely heavily on Msh3 ATPase function. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  20. A putative Lynch syndrome family carrying MSH2 and MSH6 variants of uncertain significance-functional analysis reveals the pathogenic one

    DEFF Research Database (Denmark)

    Kantelinen, Jukka; Hansen, Thomas V O; Kansikas, Minttu


    Inherited pathogenic mutations in the mismatch repair (MMR) genes, MSH2, MLH1, MSH6, and PMS2 predispose to Lynch syndrome (LS). However, the finding of a variant or variants of uncertain significance (VUS) in affected family members complicates the risk assessment. Here, we describe a putative LS...

  1. Draft Genome Sequences of Two Antimicrobial-Producing Burkholderia sp. Strains, MSh1 and MSh2, Isolated from Malaysian Tropical Peat Swamp Forest Soil (United States)

    Aw, Yoong Kit; Gan, Han Ming; Yule, Catherine M.; Lee, Sui Mae


    We report the draft genome sequences of two antimicrobial-producing isolates, Burkholderia sp. strains MSh1 and MSh2, which were isolated from tropical peat swamp forest soil. Putative genes related to different antimicrobial production have been annotated in both genome sequences. PMID:25301661

  2. α-MSH analogue attenuates blood pressure elevation in DOCA-salt hypertensive mice.

    Directory of Open Access Journals (Sweden)

    Petteri Rinne

    Full Text Available Melanocyte-stimulating hormones, α-, β- and γ-MSH, regulate important physiological functions including energy homeostasis, inflammation and sodium metabolism. Previous studies have shown that α-MSH increases sodium excretion and promotes vascular function in rodents, but it is unexplored whether these characteristics of α-MSH could translate into therapeutic benefits in the treatment of hypertension. Therefore, we first assessed the diuretic and natriuretic properties of the stable α-MSH analogue [Nle(4, D-Phe(7]-α-MSH (NDP-α-MSH and investigated whether it has protective effects in deoxycorticosterone acetate (DOCA-salt hypertensive mice. Adult male C57Bl/6N mice were subjected to DOCA-salt treatment and randomized to receive intraperitoneal injections of either saline as vehicle or NDP-α-MSH (0.3 mg/kg/day for 14 days starting 7 days after the DOCA-salt treatment. Systemic hemodynamics, serum and urine electrolytes, and oxidative stress markers were assessed in control sham-operated and DOCA-salt mice. NDP-α-MSH elicited marked diuretic and natriuretic responses that were reversible with the MC3/4 receptor antagonist SHU9119. Chronic NDP-α-MSH treatment attenuated blood pressure elevation in DOCA-salt mice without affecting the blood pressure of normotensive control animals. Owing to the enhanced sodium excretion, NDP-α-MSH-treated mice were protected from DOCA-salt-induced hypernatremia. DOCA-salt treatment mildly increased oxidative stress at the tissue level, but NDP-α-MSH had no significant effects on the oxidative stress markers. In conclusion, treatment with NDP-α-MSH increases urinary sodium excretion and protects against DOCA-salt-induced hypertension. These findings point to the potential future use of α-MSH analogues in the treatment of hypertension.

  3. Double Engine for a Nebula (United States)


    ESO has just released a stunning new image of a field of stars towards the constellation of Carina (the Keel). This striking view is ablaze with a flurry of stars of all colours and brightnesses, some of which are seen against a backdrop of clouds of dust and gas. One unusual star in the middle, HD 87643, has been extensively studied with several ESO telescopes, including the Very Large Telescope Interferometer (VLTI). Surrounded by a complex, extended nebula that is the result of previous violent ejections, the star has been shown to have a companion. Interactions in this double system, surrounded by a dusty disc, may be the engine fuelling the star's remarkable nebula. The new image, showing a very rich field of stars towards the Carina arm of the Milky Way, is centred on the star HD 87643, a member of the exotic class of B[e] stars [1]. It is part of a set of observations that provide astronomers with the best ever picture of a B[e] star. The image was obtained with the Wide Field Imager (WFI) attached to the MPG/ESO 2.2-metre telescope at the 2400-metre-high La Silla Observatory in Chile. The image shows beautifully the extended nebula of gas and dust that reflects the light from the star. The central star's wind appears to have shaped the nebula, leaving bright, ragged tendrils of gas and dust. A careful investigation of these features seems to indicate that there are regular ejections of matter from the star every 15 to 50 years. A team of astronomers, led by Florentin Millour, has studied the star HD 87643 in great detail, using several of ESO's telescopes. Apart from the WFI, the team also used ESO's Very Large Telescope (VLT) at Paranal. At the VLT, the astronomers used the NACO adaptive optics instrument, allowing them to obtain an image of the star free from the blurring effect of the atmosphere. To probe the object further, the team then obtained an image with the Very Large Telescope Interferometer (VLTI). The sheer range of this set of observations

  4. Rotationally Powered Magnetar Nebula around Swift J1834.9-0846 (United States)

    Torres, Diego F.


    A wind nebula generating extended X-ray emission was recently detected surrounding Swift J1834.9-0846. This is the first magnetar for which such a wind nebula was found. Here, we investigate whether there is a plausible scenario where the pulsar wind nebula (PWN) can be sustained without the need of advocating for additional sources of energy other than rotational. We do this by using a detailed radiative and dynamical code that studies the evolution of the nebula and its particle population in time. We find that such a scenario indeed exists: Swift J1834.9-0846's nebula can be explained as being rotationally powered, as all other known PWNe are, if it is currently being compressed by the environment. The latter introduces several effects, the most important of which is the appearance of adiabatic heating, being increasingly dominant over the escape of particles as reverberation goes by. The need of reverberation naturally explains why this is the only magnetar nebula detected and provides estimates for Swift 1834.9-0846's age.

  5. [Role of alpha-MSH and related peptides in the central nervous system]. (United States)

    Delbende, C; Jegou, S; Tranchand-Bunel, D; Leroux, P; Tonon, M C; Mocaër, E; Pelletier, G; Vaudry, H


    Alpha-melanocyte stimulating hormone (alpha-MSH) is a tridecapeptide secreted by intermediate lobe cells and synthesized in the brain as well. As a hormonal peptide, the physiological function of alpha-MSH consists mainly in the control of pigment movements within dermal melanophores. At the pituitary level, alpha-MSH secretion is under multifactorial control: it is inhibited by dopamine and GABA and stimulated by corticoliberin (CRF), thyroliberin (TRH), beta-adrenergic agonists and (or) serotonin. Identification of alpha-MSH containing neurons in the hypothalamus and other brain regions (septum, thalamus, mid-brain, striatum, hippocampus, cerebral cortex and spinal cord) has been carried out by means of immunological and biochemical techniques combined with bioassays. In the central nervous system (CNS) as in the hypophysis, alpha-MSH is synthesized from a high molecular weight precursor, pro-opiomelanocortin (POMC). Maturation of this protein yield similar end products in the hypothalamus and the intermediate lobe. Several peptides chemically related to alpha-MSH are generated including the desacetyl and monoacetyl (authentic alpha-MSH) forms; the latter has the greatest behavioral activity. The demonstration that alpha-MSH has numerous central nervous system effects has raised the possibility that this neuropeptide acts as a neuromodulator or a neurotransmitter. In the rat, intra-cerebroventricular administration of ACTH/MSH peptides induces the stretching-yawning syndrome (SYS) which is frequently preceded by excessive grooming. This excessive grooming is blocked by neuroleptics indicating that the central dopaminergic neurons are implicated in this behavioral effect of the peptide. alpha-MSH is involved in memory, arousal and attention; in hypophysectomized animals, the learning ability is restored after administration of MSH or related peptides. Injection of alpha-MSH delays also extinction of passive avoidance behavior and affects performances motivated by

  6. Ices Under Conditions of Planetary Nebulae (United States)

    Yeghikyan, A. G.


    A large number of molecules are observed in planetary nebulae, both simple, the most common (H2, CO and OH), and more complex (H2O, SiO, HCN, HNC, HCO+), and even the polycyclic aromatic hydrocarbons and fullerenes containing a few dozen and more atoms. The water molecules are observed, as a rule, in the young objects, in the gas phase (water "fountains" and related water masers) and solid phase (emission of crystalline ice particles), and, regardless of the C/O ratio, water and carbon-containing molecules may be linked to the same object. On the other hand, the results of calculations by the well known Cloudy computer program given in this paper for stationery models, show that the abundance of water ice in planetary nebulae, other conditions being equal, is dependent on the ionization rate of hydrogen, which depends in turn on the flux of energetic particles (protons and alpha particles) in the range of MeV energies and higher. The possibility of the increased flux of such particles in planetary nebulae under conditions of the standard interacting stellar winds scenario is discussed, when the flux may locally exceed by 1-3 orders of magnitude that of caused by galactic cosmic rays. Calculated water ice column densities reach values up to 1018-1019 cm-2 at the usual average ISM H2 ionisation rate of 10-16s -1 and sharply decrease for the thousands times larger rates. Known observed results of NGC 6302 show for the column density of crystalline ice about 1019cm-2 close to the calculated one.

  7. X-ray Studies of Planetary Nebulae (United States)

    Montez, Rodolfo


    X-ray emission from planetary nebulae (PNe) provides unique insight on the formation and evolution of PNe. Past observations and the ongoing Chandra Planetary Nebulae Survey (ChanPlaNS) provide a consensus on the two types of X-ray emission detected from PNe: extended and compact point-like sources. Extended X-ray emission arises from a shocked ``hot bubble'' plasma that resides within the nebular shell. Cooler than expected hot bubble plasma temperatures spurred a number of potential solutions with one emerging as the likely dominate process. The origin of X-ray emission from compact sources at the location of the central star is less clear. These sources might arise from one or combinations of the following processes: self-shocking stellar winds, spun-up binary companions, and/or accretion, perhaps from mass transfer, PN fallback, or debris disks. In the discovery phase, X-ray studies of PNe have mainly focused on the origin of the various emission processes. New directions incorporate multi-wavelength observations to study the influence of X-ray emission on the rest of the electromagnetic spectrum.

  8. MSH3-deficiency initiates EMAST without oncogenic transformation of human colon epithelial cells.

    Directory of Open Access Journals (Sweden)

    Christoph Campregher

    Full Text Available BACKGROUND/AIM: Elevated microsatellite instability at selected tetranucleotide repeats (EMAST is a genetic signature in certain cases of sporadic colorectal cancer and has been linked to MSH3-deficiency. It is currently controversial whether EMAST is associated with oncogenic properties in humans, specifically as cancer development in Msh3-deficient mice is not enhanced. However, a mutator phenotype is different between species as the genetic positions of repetitive sequences are not conserved. Here we studied the molecular effects of human MSH3-deficiency. METHODS: HCT116 and HCT116+chr3 (both MSH3-deficient and primary human colon epithelial cells (HCEC, MSH3-wildtype were stably transfected with an EGFP-based reporter plasmid for the detection of frameshift mutations within an [AAAG]17 repeat. MSH3 was silenced by shRNA and changes in protein expression were analyzed by shotgun proteomics. Colony forming assay was used to determine oncogenic transformation and double strand breaks (DSBs were assessed by Comet assay. RESULTS: Despite differential MLH1 expression, both HCT116 and HCT116+chr3 cells displayed comparable high mutation rates (about 4×10(-4 at [AAAG]17 repeats. Silencing of MSH3 in HCECs leads to a remarkable increased frameshift mutations in [AAAG]17 repeats whereas [CA]13 repeats were less affected. Upon MSH3-silencing, significant changes in the expression of 202 proteins were detected. Pathway analysis revealed overexpression of proteins involved in double strand break repair (MRE11 and RAD50, apoptosis, L1 recycling, and repression of proteins involved in metabolism, tRNA aminoacylation, and gene expression. MSH3-silencing did not induce oncogenic transformation and DSBs increased 2-fold. CONCLUSIONS: MSH3-deficiency in human colon epithelial cells results in EMAST, formation of DSBs and significant changes of the proteome but lacks oncogenic transformation. Thus, MSH3-deficiency alone is unlikely to drive human colon

  9. Multifrequency study of the ring nebula SG13 (United States)

    Vasquez, J.; Cappa, C. E.; Pineault, S.


    We investigate the morphology and kinematics of the interstellar medium in the environs of the open cluster Mrk50, which includes the Wolf-Rayet star WR157 and a number of early B-type stars. The analysis was performed using radio continuum images at 408 and 1420 MHz, and HI 21-cm line data taken from the Canadian Galactic Plane Survey, molecular observations of the 12CO (J = 1 -> 0) line at 115 GHz from the Five College Radio Astronomy Observatory and available mid- and far-infrared (FIR) observations obtained with the Midcourse Space Experiment and IRAS satellites, respectively. This study allowed the identification of the radio continuum and molecular counterpart of the ring nebula SG13, while no neutral atomic structure was found to be associated. The nebula is also detected in the images in the mid- and FIR, showing the existence of dust well mixed with the ionized gas. We estimate the main physical parameters of the material linked to the nebula. The interstellar gas distribution in the environs of Mrk50 is compatible with a stellar wind bubble created by the mass loss from WR157. The distribution of young stellar object candidates in the region shows that the stellar formation activity may be present in the molecular shell that encircles the ring nebula.

  10. HDAC6 Deacetylates and Ubiquitinates MSH2 to Maintain Proper Levels of MutSα (United States)

    Zhang, Mu; Xiang, Shengyan; Joo, Heui-Yun; Wang, Lei; Williams, Kendra A.; Liu, Wei; Hu, Chen; Tong, Dan; Haakenson, Joshua; Wang, Chuangui; Zhang, Shengping; Pavlovicz, Ryan E.; Jones, Amanda; Schmidt, Kristina H.; Tang, Jinfu; Dong, Huiqin; Shan, Bin; Fang, Bin; Radhakrishnan, Rangasudhagar; Glazer, Peter M.; Matthias, Patrick; Koomen, John; Seto, Edward; Bepler, Gerold; Nicosia, Santo V.; Chen, Jiandong; Li, Chenglong; Gu, Liya; Li, Guo-Min; Bai, Wenlong; Wang, Hengbin; Zhang, Xiaohong


    SUMMARY MutS protein homolog 2 (MSH2) is a key DNA mismatch repair protein. It forms the MSH2-MSH6 (MutSα) and MSH2-MSH3 (MutSβ) heterodimers, which help to ensure genomic integrity. MutSα not only recognizes and repairs mismatched nucleotides but also recognizes DNA adducts induced by DNA-damaging agents, and triggers cell-cycle arrest and apoptosis. Loss or depletion of MutSα from cells leads to microsatellite instability (MSI) and resistance to DNA damage. Although the level of MutSα can be reduced by the ubiquitin-proteasome pathway, the detailed mechanisms of this regulation remain elusive. Here we report that histone deacetylase 6 (HDAC6) sequentially deacetylates and ubiquitinates MSH2, leading to MSH2 degradation. In addition, HDAC6 significantly reduces cellular sensitivity to DNA-damaging agents and decreases cellular DNA mismatch repair activities by downregulation of MSH2. Overall, these findings reveal a mechanism by which proper levels of MutSα are maintained. PMID:24882211

  11. Mechanisms by which the orexigen NPY regulates anorexigenic α-MSH and TRH. (United States)

    Cyr, Nicole E; Toorie, Anika M; Steger, Jennifer S; Sochat, Matthew M; Hyner, Samantha; Perello, Mario; Stuart, Ronald; Nillni, Eduardo A


    Protein posttranslational processing is a cellular mechanism fundamental to the generation of bioactive peptides, including the anorectic α-melanocyte-stimulating hormone (α-MSH) and thyrotropin-releasing hormone (TRH) peptides produced in the hypothalamic arcuate (ARC) and paraventricular (PVN) nuclei, respectively. Neuropeptide Y (NPY) promotes positive energy balance in part by suppressing α-MSH and TRH. The mechanism by which NPY regulates α-MSH output, however, is not well understood. Our results reveal that NPY inhibited the posttranslational processing of α-MSH's inactive precursor proopiomelanocortin (POMC) by decreasing the prohormone convertase-2 (PC2). We also found that early growth response protein-1 (Egr-1) and NPY-Y1 receptors mediated the NPY-induced decrease in PC2. NPY given intra-PVN also decreased PC2 in PVN samples, suggesting a reduction in PC2-mediated pro-TRH processing. In addition, NPY attenuated the α-MSH-induced increase in TRH production by two mechanisms. First, NPY decreased α-MSH-induced CREB phosphorylation, which normally enhances TRH transcription. Second, NPY decreased the amount of α-MSH in the PVN. Collectively, these results underscore the significance of the interaction between NPY and α-MSH in the central regulation of energy balance and indicate that posttranslational processing is a mechanism that plays a specific role in this interaction.

  12. Pinwheel Nebula around WR 98a. (United States)

    Monnier; Tuthill; Danchi


    We present the first near-infrared images of the dusty Wolf-Rayet star WR 98a. Aperture-masking interferometry has been utilized to recover images at the diffraction limit of the Keck I telescope, less, similar50 mas at 2.2 µm. Multiepoch observations spanning about 1 yr have resolved the dust shell into a "pinwheel" nebula, the second example of a new class of dust shell first discovered around WR 104 by Tuthill, Monnier, & Danchi. Interpreting the collimated dust outflow in terms of an interacting winds model, the binary orbital parameters and apparent wind speed are derived: a period of 565+/-50 days, a viewing angle of 35&j0;+/-6 degrees from the pole, and a wind speed of 99+/-23 mas yr-1. This period is consistent with a possible approximately 588 day periodicity in the infrared light curve, linking the photometric variation to the binary orbit. Important implications for binary stellar evolution are discussed by identifying WR 104 and WR 98a as members of a class of massive, short-period binaries whose orbits were circularized during a previous red supergiant phase. The current component separation in each system is similar to the diameter of a red supergiant, which indicates that the supergiant phase was likely terminated by Roche lobe overflow, leading to the present Wolf-Rayet stage.

  13. MSH6 Mutations are Frequent in Hereditary Nonpolyposis Colorectal Cancer Families With Normal pMSH6 Expression as Detected by Immunohistochemistry

    DEFF Research Database (Denmark)

    Okkels, Henrik; Larsen, K.L.; Thorlacius-Ussing, O.


    Hereditary nonpolyposis colorectal cancer (HNPCC) is an autosomal dominant condition accounting for 2% to 4% of all colorectal cancer cases worldwide. Families with germ line mutations in 1 of 6 mismatch repair genes are known as Lynch syndrome families. The largest number of mutations has been...... detected in the mismatch repair genes MLH1 and MSH2, but several mutations in MSH6 have also been demonstrated....

  14. Dust in planetary nebulae (United States)

    Sloan, G. C.


    Infrared spectra from the Spitzer Space Telescope trace the evolution of carbon-rich dust from the asymptotic giant branch (AGB) to young planetary nebulae (PNe). On the AGB, amorphous carbon dominates the dust, but SiC and MgS also appear. In more evolved systems with warmer central stars, the spectra reveal the unidentified 21 μm feature, features from aliphatic hydrocarbons, and spectra from polycyclic aromatic hydrocarbons (PAHs), often with shifted feature positions indicative of the presence of aliphatics. More evolved systems with hot central stars show more typical PAH spectra, along with fullerenes and/or an emission feature known as the big-11 feature at ~11 μm. This features arises from a combination of SiC and PAHs, and it is usually accompanied by a shoulder at 18 μm, which while unidentified might be from cool silicate grains. The strong emission from MgS and SiC in young PNe probably arises from coatings on carbonaceous grains.

  15. MSH2 and CXCR4 involvement in malignant VIPoma

    Directory of Open Access Journals (Sweden)

    Müller Sven


    Full Text Available Abstract Background Vasoactive intestinal polypeptide secreting tumors(VIPomas are rare endocrine tumors of the pancreas with an estimated incidence of 0.1 per million per year. The molecular mechanisms that mediate development of VIPomas are poorly investigated and require definition. Methods A genome- and gene expression analysis of specimens of a primary pancreatic VIPoma with hepatic metastases was performed. The primary tumor, the metastases, the corresponding healthy tissue of the liver, and the pancreas were compared with each other using oligonucleotide microarrays and loss of heterozygosity (LOH. Results The results revealed multiple LOH events and several differentially expressed genes. Our finding of LOH and downregulation was conspicuous in the microarray analysis for the mismatch repair gene MSH2 in the primary pancreatic VIPoma tumor, the hepatic metastasis but not in the corresponding healthy tissue. Further a strong overexpression of the chemokine CXCR4 was detected in the hepatic metastases compared to its pancreatic primary. With a review of the literature we describe the molecular insights of metastatic development in VIPoma. Conclusion In VIPoma, defects in the mismatch repair system especially in MSH2 may contribute to carcinogenesis, and increased CXCR4 may be associated with liver metastasis.

  16. A putative Lynch syndrome family carrying MSH2 and MSH6 variants of uncertain significance-functional analysis reveals the pathogenic one

    DEFF Research Database (Denmark)

    Kantelinen, Jukka; Hansen, Thomas V O; Kansikas, Minttu


    Inherited pathogenic mutations in the mismatch repair (MMR) genes, MSH2, MLH1, MSH6, and PMS2 predispose to Lynch syndrome (LS). However, the finding of a variant or variants of uncertain significance (VUS) in affected family members complicates the risk assessment. Here, we describe a putative LS...... family carrying VUS in both MSH2 (c.2768T>A, p.Val923Glu) and MSH6 (c.3563G>A, p.Ser1188Asn). Two colorectal cancer (CRC) patients were studied for mutations and identified as carriers of both variants. In spite of a relatively high mean age of cancer onset (59.5 years) in the family, many CRC patients...... and the tumor pathological data suggested that the missense variation in MSH2, the more common susceptibility gene in LS, would be the predisposing alteration. However, MSH2 VUS was surprisingly found to be MMR proficient in an in vitro MMR assay and a tolerant alteration in silico. By supplying evidence...

  17. Functional analysis of HNPCC-related missense mutations in MSH2

    Energy Technology Data Exchange (ETDEWEB)

    Luetzen, Anne [Department of Science, Systems and Models, Roskilde University, DK-4000 Roskilde (Denmark); Wind, Niels de; Georgijevic, Dubravka [Department of Toxicogenetics, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden (Netherlands); Nielsen, Finn Cilius [Department of Clinical Biochemistry, Rigshospitalet, DK-2100 Copenhagen (Denmark); Rasmussen, Lene Juel [Department of Science, Systems and Models, Roskilde University, DK-4000 Roskilde (Denmark)], E-mail:


    Hereditary nonpolyposis colorectal cancer (HNPCC) is associated with germline mutations in the human DNA mismatch repair (MMR) genes, most frequently MSH2 and MLH1. The majority of HNPCC mutations cause truncations and thus loss of function of the affected polypeptide. However, a significant proportion of MMR mutations found in HNPCC patients are single amino acid substitutions and the functional consequences of many of these mutations in DNA repair are unclear. We have examined the consequences of seven MSH2 missense mutations found in HNPCC families by testing the MSH2 mutant proteins in functional assays as well as by generating equivalent missense mutations in Escherichia coli MutS and analyzing the phenotypes of these mutants. Here we show that two mutant proteins, MSH2-P622L and MSH2-C697F confer multiple biochemical defects, namely in mismatch binding, in vivo interaction with MSH6 and EXO1, and in nuclear localization in the cell. Mutation G674R, located in the ATP-binding region of MSH2, appears to confer resistance to ATP-dependent mismatch release. Mutations D167H and H639R show reduced mismatch binding. Results of in vivo experiments in E. coli with MutS mutants show that one additional mutant, equivalent of MSH2-A834T that do not show any defects in MSH2 assays, is repair deficient. In conclusion, all mutant proteins (except for MSH2-A305T) have defects; either in mismatch binding, ATP-release, mismatch repair activity, subcellular localization or protein-protein interactions.

  18. The Formation of a Planetary Nebula. (United States)

    Harpaz, Amos


    Proposes a scenario to describe the formation of a planetary nebula, a cloud of gas surrounding a very hot compact star. Describes the nature of a planetary nebula, the number observed to date in the Milky Way Galaxy, and the results of research on a specific nebula. (MDH)

  19. Wind Braking of Magnetars (United States)

    Tong, H.; Xu, R. X.; Song, L. M.; Qiao, G. J.


    We explore the wind braking of magnetars considering recent observations challenging the traditional magnetar model. There is evidence for strong multipole magnetic fields in active magnetars, but the dipole field inferred from spin-down measurements may be strongly biased by particle wind. Recent observations challenging the traditional model of magnetars may be explained naturally by the wind braking scenario: (1) the supernova energies of magnetars are of normal value; (2) the non-detection in Fermi observations of magnetars; (3) the problem posed by low magnetic field soft gamma-ray repeaters; (4) the relation between magnetars and high magnetic field pulsars; and (5) a decreasing period derivative during magnetar outbursts. Transient magnetars with L_x{<}-\\dot{E}_rot may still be magnetic dipole braking. This may explain why low luminosity magnetars are more likely to have radio emissions. A strong reduction of the dipole magnetic field is possible only when the particle wind is very collimated at the star surface. A small reduction of the dipole magnetic field may result from detailed considerations of magnetar wind luminosity. In the wind braking scenario, magnetars are neutron stars with a strong multipole field. For some sources, a strong dipole field may no longer be needed. A magnetism-powered pulsar wind nebula will be one of the consequences of wind braking. For a magnetism-powered pulsar wind nebula, we should see a correlation between the nebula luminosity and the magnetar luminosity. Under the wind braking scenario, a braking index smaller than three is expected. Future braking index measurement of a magnetar may tell us whether magnetars are wind braking or magnetic dipole braking.

  20. Modelling the ArH+ emission from the Crab nebula (United States)

    Priestley, F. D.; Barlow, M. J.; Viti, S.


    We have performed combined photoionization and photodissociation region (PDR) modelling of a Crab nebula filament subjected to the synchrotron radiation from the central pulsar wind nebula, and to a high flux of charged particles; a greatly enhanced cosmic-ray ionization rate over the standard interstellar value, ζ0, is required to account for the lack of detected [C I] emission in published Herschel SPIRE FTS observations of the Crab nebula. The observed line surface brightness ratios of the OH+ and ArH+ transitions seen in the SPIRE FTS frequency range can only be explained with both a high cosmic-ray ionization rate and a reduced ArH+ dissociative recombination rate compared to that used by previous authors, although consistent with experimental upper limits. We find that the ArH+/OH+ line strengths and the observed H2 vibration-rotation emission can be reproduced by model filaments with nH = 2 × 104 cm-3, ζ = 107ζ0 and visual extinctions within the range found for dusty globules in the Crab nebula, although far-infrared emission from [O I] and [C II] is higher than the observational constraints. Models with nH = 1900 cm-3 underpredict the H2 surface brightness, but agree with the ArH+ and OH+ surface brightnesses and predict [O I] and [C II] line ratios consistent with observations. These models predict HeH+ rotational emission above detection thresholds, but consideration of the formation time-scale suggests that the abundance of this molecule in the Crab nebula should be lower than the equilibrium values obtained in our analysis.

  1. Structural, molecular and cellular functions of MSH2 and MSH6 during DNA mismatch repair, damage signaling and other noncanonical activities

    Energy Technology Data Exchange (ETDEWEB)

    Edelbrock, Michael A., E-mail: [The University of Findlay, 1000 North Main Street, Findlay, OH 45840 (United States); Kaliyaperumal, Saravanan, E-mail: [Division of Comparative Medicine and Pathology, New England Primate Research Center, One Pine Hill Drive, Southborough, MA 01772 (United States); Williams, Kandace J., E-mail: [University of Toledo College of Medicine and Life Sciences, Department of Biochemistry and Cancer Biology, 3000 Transverse Dr., Toledo, OH 43614 (United States)


    The field of DNA mismatch repair (MMR) has rapidly expanded after the discovery of the MutHLS repair system in bacteria. By the mid 1990s yeast and human homologues to bacterial MutL and MutS had been identified and their contribution to hereditary non-polyposis colorectal cancer (HNPCC; Lynch syndrome) was under intense investigation. The human MutS homologue 6 protein (hMSH6), was first reported in 1995 as a G:T binding partner (GTBP) of hMSH2, forming the hMutSα mismatch-binding complex. Signal transduction from each DNA-bound hMutSα complex is accomplished by the hMutLα heterodimer (hMLH1 and hPMS2). Molecular mechanisms and cellular regulation of individual MMR proteins are now areas of intensive research. This review will focus on molecular mechanisms associated with mismatch binding, as well as emerging evidence that MutSα, and in particular, MSH6, is a key protein in MMR-dependent DNA damage response and communication with other DNA repair pathways within the cell. MSH6 is unstable in the absence of MSH2, however it is the DNA lesion-binding partner of this heterodimer. MSH6, but not MSH2, has a conserved Phe-X-Glu motif that recognizes and binds several different DNA structural distortions, initiating different cellular responses. hMSH6 also contains the nuclear localization sequences required to shuttle hMutSα into the nucleus. For example, upon binding to O{sup 6}meG:T, MSH6 triggers a DNA damage response that involves altered phosphorylation within the N-terminal disordered domain of this unique protein. While many investigations have focused on MMR as a post-replication DNA repair mechanism, MMR proteins are expressed and active in all phases of the cell cycle. There is much more to be discovered about regulatory cellular roles that require the presence of MutSα and, in particular, MSH6.

  2. Formation of magnesium silicate hydrate (M-S-H) cement pastes using sodium hexametaphosphate

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Tingting [Faculty of Infrastructure Engineering, Dalian University of Technology, Dalian 116024 (China); Department of Materials, Centre for Advanced Structural Ceramics, Imperial College London, South Kensington Campus, London SW7 2AZ (United Kingdom); Department of Civil and Environmental Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ (United Kingdom); Vandeperre, Luc J. [Department of Materials, Centre for Advanced Structural Ceramics, Imperial College London, South Kensington Campus, London SW7 2AZ (United Kingdom); Cheeseman, Christopher R., E-mail: [Department of Civil and Environmental Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ (United Kingdom)


    Magnesium silicate hydrate (M-S-H) gel is formed by the reaction of brucite with amorphous silica during sulphate attack in concrete and M-S-H is therefore regarded as having limited cementing properties. The aim of this work was to form M-S-H pastes, characterise the hydration reactions and assess the resulting properties. It is shown that M-S-H pastes can be prepared by reacting magnesium oxide (MgO) and silica fume (SF) at low water to solid ratio using sodium hexametaphosphate (NaHMP) as a dispersant. Characterisation of the hydration reactions by x-ray diffraction and thermogravimetric analysis shows that brucite and M-S-H gel are formed and that for samples containing 60 wt.% SF and 40 wt.% MgO all of the brucites react with SF to form M-S-H gel. These M-S-H cement pastes were found to have compressive strengths in excess of 70 MPa.

  3. The natural history of a combined defect in MSH6 and MUTYH in a HNPCC family. (United States)

    van Puijenbroek, Marjo; Nielsen, Maartje; Reinards, Tjitske H C M; Weiss, Marjan M; Wagner, Anja; Hendriks, Yvonne M C; Vasen, Hans F A; Tops, Carli M J; Wijnen, Juul; van Wezel, Tom; Hes, Frederik J; Morreau, Hans


    In the inherited syndromes, MUTYH-associated polyposis (MAP) and hereditary nonpolyposis colorectal cancer (HNPCC), somatic mutations occur due to loss of the caretaker function that base-repair (BER) and mismatch repair (MMR) genes have, respectively. Recently, we identified a large branch from a MSH6 HNPCC family in which 19 family members are heterozygous or compound heterozygous for MUTYH germ line mutations. MSH6/MUTYH heterozygote mutation carriers display a predominant HNPCC molecular tumour phenotype, with microsatellite instability and underrepresentation of G>T transversions. A single unique patient is carrier of the MSH6 germline mutation and is compound heterozygote for MUTYH. Unexpectedly, this patient has an extremely mild clinical phenotype with sofar only few adenomas at age 56. Four out of five adenomas show characteristic G>T transversions in APC and/or KRAS2, as seen in MUTYH associated polyposis. No second hit of MSH6 is apparent in any of the adenomas, due to retained MSH6 nuclear expression and a lack of microsatellite instability. Although this concerns only one case, we argue that the chance to find an additional one is extremely small and currently a mouse model with this genotype combination is not available. Moreover, the patients brother who is also compound heterozygous for MUTYH but lacks the MSH6 germline mutation presented with a full blown polyposis coli. In conclusion, these data would support the notion that abrogation of both MSH6 DNA mismatch repair and base repair might be mutually exclusive in humans.

  4. The mouse mismatch repair protein, MSH3, is a nucleoplasmic protein that aggregates into denser nuclear bodies under conditions of stress.

    NARCIS (Netherlands)

    Holt, I.; Thanh Lam, L.; Tome, S.; Wansink, D.G.; Riele, H. te; Gourdon, G.; Morris, G.E.


    The mismatch repair protein, MSH3, together with MSH2, forms the MutSbeta heterodimer which recognizes and repairs base pair mismatches and larger insertion/deletion loops in DNA. Lack of specific antibodies against mouse MSH3 has hampered studies of its expression and localization. Mouse MSH3 is

  5. A Model for the Dynamical and Ionization Structure of Planetary Nebula IC 418

    Directory of Open Access Journals (Sweden)

    J. Ghanbari


    Full Text Available   The interacting two winds model and a spherical density distribution function are introduced to study the dynamical and ionization structure of the planetary nebula IC 418. A fast wind with a mechanical luminousity  2/34×1034erg.s-1 of interacts with a super wind with a mass-loss rate of  2×10-5M(°yr-1 and  a velocity of 10 , and produces a dense and luminous medium.   In this model, the expansion velocities of OI and HI lines are predicted to be 11 and 10.5kms-1 , respectively. The calculated dynamical time-scale 1033yr for the nebula is in good agreement with the evolution time of the central star after the interaction of the two winds. Our calculations give a luminosity  0.05M(°of for the central star

  6. β-Endorphin Antagonizes the Effects of α-MSH on Food Intake and Body Weight


    Dutia, Roxanne; Meece, Kana; Dighe, Shveta; Andrea J. Kim; Wardlaw, Sharon L.


    Proopiomelanocortin (POMC) is posttranslationally processed to several peptides including α-MSH, a primary regulator of energy balance that inhibits food intake and stimulates energy expenditure. However, another POMC-derived peptide, β-endorphin (β-EP), has been shown to stimulate food intake. In this study we examined the effects of intracerebroventricular (icv) β-EP on food intake and its ability to antagonize the negative effects of α-MSH on energy balance in male rats. A single icv injec...

  7. Alpha-MSH, the melanocortin-1 receptor and background adaptation in the Mozambique tilapia, Oreochromis mossambicus. (United States)

    van der Salm, A L; Metz, J R; Bonga, S E Wendelaar; Flik, G


    The regulation of skin darkness in vertebrates is mediated by alpha-melanophore-stimulating-hormone (alphaMSH). For this action, alphaMSH binds to the melanocortin (MC)-1 receptor, a 7-transmembrane receptor located in melanophore cell membranes. The Mozambique tilapia, Oreochromis mossambicus, can change the hue of its body in response to a change in background, a process that may involve alphaMSH and the MC1R. Scale melanophores were isolated from tilapia that were acclimatised for 25 days to a black, control grey or white background and then tested for their sensitivity to des-, mono-, and di-acetylated alphaMSH. On all backgrounds, mono-acetylated alphaMSH was the dominant isoform present in pituitary homogenates. Mono-acetylated alphaMSH also had the highest potency to disperse melanosomes. Black background adapted fish showed the highest dispersing response to alphaMSH, independent of the isoform applied. We elucidated the nucleotide and amino acid sequence of the tilapia MC1R. We show that its expression in skin does not change when tilapia are acclimatised for 25 days to a black, grey or white background, while a clear change in hue is visible. This finding, combined with the absence of differential MC1R gene expression following background acclimation indicates that the increased sensitivity to alphaMSH is most likely a result of changes in the intracellular signalling system in melanophores of black background adapted fish, rather than up-regulation of the MC1R.

  8. Immunohistochemical detection of acth and msh cells in the hypophysis of the hermaphroditic teleost, Diplodus sargus

    Directory of Open Access Journals (Sweden)

    M Consiglio Grimaldi


    Full Text Available Hypophyseal ACTH and MSH cells were immunohistochemically characterised in the teleost fish, Diplodus sargus, using anti-ACTH (1-24 and anti a-MSH polyclonal antisera. ACTH cells were found both in the pars distalis and in the pars intermedia. In the former region, they appeared small, round-shaped and clustered; in the latter, they were either small or large and elongated. Moreover, a few ACTH-immunoreactive cells resembling microglia were present in the neurohypophysis. Conversely, MSH cells were found only in the pars intermedia, and were similar to the larger ACTH cells of the same region. In the pars intermedia, co-localisation of ACTH and MSH immunoreactivity in the same cell was revealed by double immunostaining, though the two hormones were also observed in distinct cell types. The distribution of ACTH cells appeared quite uniform, without any marked difference between the specimens tested. Conversely, MSH cell amount varied according to the stage of the sexual cycle of this teleost fish, which is characterised by protandrous hermaphroditism. In fact, a lower amount of MSH cells were observed in females, whereas no significant difference was found between immature and male specimens.

  9. Aminobacter MSH1-Mineralisation of BAM in Sand-Filters Depends on Biological Diversity. (United States)

    Ekelund, Flemming; Harder, Christoffer Bugge; Knudsen, Berith Elkær; Aamand, Jens


    BAM (2,6-dichlorobenzamide) is a metabolite of the pesticide dichlobenil. Naturally occurring bacteria that can utilize BAM are rare. Often the compound cannot be degraded before it reaches the groundwater and therefore it poses a serious threat to drinking water supplies. The bacterial strain Aminobacter MSH1 is a BAM degrader and therefore a potential candidate to be amended to sand filters in waterworks to remediate BAM polluted drinking water. A common problem in bioremediation is that bacteria artificially introduced into new diverse environments often thrive poorly, which is even more unfortunate because biologically diverse environments may ensure a more complete decomposition. To test the bioaugmentative potential of MSH1, we used a serial dilution approach to construct microcosms with different biological diversity. Subsequently, we amended Aminobacter MSH1 to the microcosms in two final concentrations; i.e. 10(5) cells mL(-1) and 10(7) cells mL(-1). We anticipated that BAM degradation would be most efficient at "intermediate diversities" as low diversity would counteract decomposition because of incomplete decomposition of metabolites and high diversity would be detrimental because of eradication of Aminobacter MSH1. This hypothesis was only confirmed when Aminobacter MSH1 was amended in concentrations of 10(5) cells mL(-1). Our findings suggest that Aminobacter MSH1 is a very promising bioremediator at several diversity levels.

  10. Spectroscopy of the ringlike nebula toward the open cluster NGC 3572 (United States)

    Noumaru, Junichi; Ogura, Katsuo


    Low-dispersion spectroscopy has been obtained for the ringlike nebula which Phelps and Janes (1991) found in the direction of the young open cluster NGC 3572 and suspected as a planetary nebula. Some nearby nebulosities have also been observed. Analyses of these data indicate that all of them, including the NGC 3572 ring, are H II regions. Morphological considerations of the region show that the nearby nebulosities are bright rims which are associated with the H II region BBW 342 and are partly hidden by the obscuring matter lying on this side. The NGC 3572 ring could be of the same nature. However, as the alternative interpretation, it could be a ring nebula (probably a wind-blown bubble) around a massive star (WR/Of star or LBV).

  11. Detonative propagation and accelerative expansion of the Crab Nebula shock front. (United States)

    Gao, Yang; Law, Chung K


    The accelerative expansion of the Crab Nebula's outer envelope is a mystery in dynamics, as a conventional expanding blast wave decelerates when bumping into the surrounding interstellar medium. Here we show that the strong relativistic pulsar wind bumping into its surrounding nebula induces energy-generating processes and initiates a detonation wave that propagates outward to form the current outer edge, namely, the shock front, of the nebula. The resulting detonation wave, with a reactive downstream, then provides the needed power to maintain propagation of the shock front. Furthermore, relaxation of the curvature-induced reduction of the propagation velocity from the initial state of formation to the asymptotic, planar state of Chapman-Jouguet propagation explains the observed accelerative expansion. Potential richness in incorporating reactive fronts in the description of various astronomical phenomena is expected. © 2011 American Physical Society

  12. The Extended X-ray Nebula of PSR J1420-6048

    Energy Technology Data Exchange (ETDEWEB)

    Van Etten, Adam; Romani, Roger W.; /Stanford U., Phys. Dept.


    The vicinity of the unidentified EGRET source 3EG J1420-6038 has undergone extensive study in the search for counterparts, revealing the energetic young pulsar PSR J1420-6048 and its surrounding wind nebula as a likely candidate for at least part of the emission from this bright and extended gamma-ray source. We report on new Suzaku observations of PSR J1420-6048, along with analysis of archival XMM Newton data. The low background of Suzaku permits mapping of the extended X-ray nebula, indicating a tail stretching {approx} 8 minutes north of the pulsar. The X-ray data, along with archival radio and VHE data, hint at a pulsar birthsite to the North, and yield insights into its evolution and the properties of the ambient medium. We further explore such properties by modeling the spectral energy distribution (SED) of the extended nebula.

  13. Avian melanocortin system: alpha-MSH may act as an autocrine/paracrine hormone: a minireview. (United States)

    Takeuchi, Sakae; Takahashi, Sumio; Okimoto, Ronald; Schioth, Helgi B; Boswell, Timothy


    The interest in the physiological role of alpha-MSH in birds has been limited because they lack the intermediate lobe of the pituitary, the main source of circulating alpha-MSH in most vertebrates. Recent studies have improved our understanding of the avian melanocortin system. We have cloned and characterized all five MC-R subtypes, POMC, and AGRP in chicken. Analyses of the tissue distribution of expression of these genes revealed widespread expression throughout the body, corresponding to the situation in mammals in which alpha-MSH exerts a multiplicity of effects in different tissues by acting as a local mediator. We showed that the extended black locus controlling feather pigmentation in the chicken encodes MC1-R. Moreover, black chickens carrying the dominant allele, the extended black, express the MC1-R with ligand-independent activity as the somber-3J black mice. alpha-MSH and AGRP were expressed in the infundibular nucleus of POMC and NPY neurons, respectively, in the brain of Japanese quail. Furthermore, fasting stimulated AGRP expression and lowered POMC expression. These data indicate that at least two of the major melanocortin systems reported in mammals, that is, regulation of pigmentation and energy homeostasis, was developed in a common ancestor to chicken and mammals at least 300 million years ago. Furthermore, alpha-MSH peptide was identified in developing chicken eye, suggesting a possible involvement of alpha-MSH in regulation of ocular development. Collectively, the data reviewed here indicate that alpha-MSH is produced locally and acts as an autocrine/paracrine hormone in birds.

  14. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells.

    Directory of Open Access Journals (Sweden)

    Jae Myung Park

    Full Text Available MSH3 is a DNA mismatch repair (MMR gene that undergoes frequent somatic mutation in colorectal cancers (CRCs with MMR deficiency. MSH3, together with MSH2, forms the MutSβ heteroduplex that interacts with interstrand cross-links induced by drugs such as cisplatin. To date, the impact of MSH3 on chemosensitivity is unknown.We utilized isogenic HCT116 (MLH1-/MSH3- cells where MLH1 is restored by transfer of chromosome 3 (HCT116+ch3 and also MSH3 by chromosome 5 (HCT116+3+5. We generated HCT116+3+5, SW480 (MLH1+/MSH3+ and SW48 (MLH1-/MSH3+ cells with shRNA knockdown of MSH3. Cells were treated with 5-fluorouracil (5-FU, SN-38, oxaliplatin, or the histone deacetylase (HDAC inhibitor PCI-24781 and cell viability, clonogenic survival, DNA damage and apoptosis were analyzed.MSH3-deficient vs proficient CRC cells showed increased sensitivity to the irinotecan metabolite SN-38 and to oxaliplatin, but not 5-FU, as shown in assays for apoptosis and clonogenic survival. In contrast, suppression of MLH1 attenuated the cytotoxic effect of 5-FU, but did not alter sensitivity to SN-38 or oxaliplatin. The impact of MSH3 knockdown on chemosensitivity to SN-38 and oxaliplatin was maintained independent of MLH1 status. In MSH3-deficient vs proficient cells, SN-38 and oxaliplatin induced higher levels of phosphorylated histone H2AX and Chk2, and similar results were found in MLH1-proficient SW480 cells. MSH3-deficient vs proficient cells showed increased 53BP1 nuclear foci after irradiation, suggesting that MSH3 can regulate DNA double strand break (DSB repair. We then utilized PCI-24781 that interferes with homologous recombination (HR indicated by a reduction in Rad51 expression. The addition of PCI-24781 to oxaliplatin enhanced cytotoxicity to a greater extent compared to either drug alone.MSH3 status can regulate the DNA damage response and extent of apoptosis induced by chemotherapy. The ability of MSH3 to regulate chemosensitivity was independent of MLH1

  15. Challenges in the identification of MSH6-associated colorectal cancer: rectal location, less typical histology, and a subset with retained mismatch repair function

    DEFF Research Database (Denmark)

    Klarskov, Louise; Holck, Susanne; Bernstein, Inge


    with MLH1/MSH2-mutant tumors and sporadic mismatch repair-deficient cancers. In the MSH6 subset, we confirmed a higher age (median, 56 y) at diagnosis and found a significantly larger proportion (25%) of rectal cancers. Presence of dirty necrosis was the sole histologic component that significantly...... differed between MSH6 and MLH1/MSH2 tumors. Compared with the sporadic mismatch repair-defective cohort, MSH6 cases had a lower prevalence of tumor-infiltrating lymphocytes and Crohn-like reactions. Mismatch repair defects were identified in 92% of MSH6 tumors, with high concordance between microsatellite...

  16. Properties and Spatial Distribution of Dust Emission in the Crab Nebula (United States)

    Sonneborn, G.; Temim, T.; Dwek, E.; Arendt, R.; Gehrz, R.; Slane, P.


    The nature and quantity of dust produced in supernovae (SNe) is still poorly understood. Recent IR observations of freshly-formed dust in supernova remnants (SNRs) have yielded significantly lower dust masses than predicted by theoretical models and observations high-redshift galaxies. The Crab Nebula's pulsar wind is thought to be sweeping up freshly-formed SN dust along with the SN ejecta. The evidence for this dust was found in the form of an IR bump in the integrated spectrum of the Crab and in extinction against the synchrotron nebula that revealed the presence of dust in the filament cores. We present the first spatially-resolved emission spectra of dust in the Crab Nebula acquired with the Spitzer Space Telescope. The IR spectra are dominated by synchrotron emission and show forbidden line emission from both sides of the expanding nebula, including emission from [S III], [Si II], [Ne II], [Ne III], [Ne V], [Ar III], [Ar V], [Fe II], and [Ni II]. We extrapolated a synchrotron spectral data cube from the Spitzer 3.6 and 4.5 micron images, and subtracted this contribution from our 15-40 micron spectral data to produce a map of the residual continuum emission from dust. The emission appears to be concentrated along the ejecta filaments and is well described by astronomical silicates at an average temperature of 65 K. The estimated mass of dust in the Crab Nebula is 0.008 solar masses.

  17. Photoionisation effects in hydrodynamical simulations of planetary nebulae (United States)

    Hernández-Martínez, L.; Estrella, D.; Velázquez, P. F.; Esquivel, A.; Raga, A. C.


    We explored the photoionisation effects on both the proper motion and emission of planetary nebulae NGC 6302, by means of hydrodynamical simulations. We used the GUACHO code, which includes the photoionisation due to central source (Esquivel et al. 2009, Esquivel & Raga 2013). We model these PNe considering an interacting stellar fast wind with and ejected toroidally shaped slow wind (Uscanga et al. 2014). Synthetic Hα emission maps were obtained from our numerical results in order to do a comparison between the cases with and without photoionisation. Using a wavelets fittering method on our results for the ionisation case, we do not find an increase in the proper motion velocities, however we can see an accelerated expansion in both cases. For the ionisation case the Hα emission presents an increase.

  18. Using Planetary Nebulae to Teach Physics (United States)

    Kwitter, Karen B.


    We have developed an interactive website, "Gallery of Planetary Nebula Spectra," ( that contains high-quality optical-to-near-infrared spectra, atlas information, and bibliographic references for more than 160 planetary nebulae that we have observed in the Milky Way Galaxy. To make the material more accessible to students, I have created three undergraduate-level exercises that explore physics-related aspects of planetary nebulae. "Emission Lines and Central Star Temperature” uses the presence or absence of emission lines from species with different ionization potentials to rank the temperatures of the exciting stars in a selection of nebulae. "Interstellar Reddening” uses the observed Balmer decrement in a sample of planetary nebulae at different Galactic latitudes to infer the distribution of interstellar dust in the Milky Way. Finally, "Determining the Gas Density in Planetary Nebulae,” which I will focus on here, uses the observed intensity ratio of the 6717 Å and 6731 Å emission lines from singly ionized sulfur to determine the electron density in the nebular gas. These exercises demonstrate that planetary nebula spectra are useful real-world examples illustrating a variety of physical principles, including the behavior of blackbodies, wavelength-dependent particle scattering, recombination-line ratios, atomic physics, and statistical mechanics.

  19. Optical observations of southern planetary nebula candidates

    NARCIS (Netherlands)

    VandeSteene, GC; Sahu, KC; Pottasch, [No Value


    We present H alpha+[NII] images and low resolution spectra of 16 IRAS-selected, southern planetary nebula candidates previously detected in the radio continuum. The H alpha+[NII] images are presented as finding charts. Contour plots are shown for the resolved planetary nebulae. From these images

  20. Abundances of planetary nebula NGC 5315

    NARCIS (Netherlands)

    Pottasch, [No Value; Beintema, DA; Salas, JB; Koornneef, J; Feibelman, WA


    The ISO and IUE spectra of the elliptical nebula NGC 5315 is presented. These spectra are combined with the spectra in the visual wavelength region to obtain a complete, extinction corrected, spectrum. The chemical composition of the nebulae is then calculated and compared to previous

  1. An x-ray nebula associated with the millisecond pulsar B1957+20. (United States)

    Stappers, B W; Gaensler, B M; Kaspi, V M; van der Klis, M; Lewin, W H G


    We have detected an x-ray nebula around the binary millisecond pulsar B1957+20. A narrow tail, corresponding to the shocked pulsar wind, is seen interior to the known Halpha bow shock and proves the long-held assumption that the rotational energy of millisecond pulsars is dissipated through relativistic winds. Unresolved x-ray emission likely represents the shock where the winds of the pulsar and its companion collide. This emission indicates that the efficiency with which relativistic particles are accelerated in the postshock flow is similar to that for young pulsars, despite the shock proximity and much weaker surface magnetic field of this millisecond pulsar.

  2. Cadmium(Cd)-induced oxidative stress down-regulates the gene expression of DNA mismatch recognition proteins MutS homolog 2 (MSH2) and MSH6 in zebrafish (Danio rerio) embryos

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, Todd, E-mail: [Institute of Bioscience and Biotechnology and Center of Excellence for Marine Bioenvironment and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan (China); Huang, Kuan-Ming; Tsai, Huei-Ting; Sung, Shih-Tsung; Ho, Tsung-Nan [Institute of Bioscience and Biotechnology and Center of Excellence for Marine Bioenvironment and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan (China)


    DNA mismatch repair (MMR) of simple base mismatches and small insertion-deletion loops in eukaryotes is initiated by the binding of the MutS homolog 2 (MSH2)-MSH6 heterodimer to mismatched DNA. Cadmium (Cd) is a genotoxic heavy metal that has been recognized as a human carcinogen. Oxidant stress and inhibition of DNA repair have been proposed as major factors underlying Cd genotoxicity. Our previous studies indicated the ability of Cd to disturb the gene expression of MSH6 in zebrafish (Danio rerio) embryos. This study was undertaken to explore if Cd-induced oxidative stress down-regulated MSH gene activities. Following the exposure of zebrafish embryos at 1 h post fertilization (hpf) to sublethal concentrations of Cd at 3-5 {mu}M for 4 or 9 h, a parallel down-regulation of MSH2, MSH6 and Cu/Zn superoxide dismutase (Cu/Zn-SOD) gene expression was detected by real-time RT-PCR and the expression levels were 40-50% of control after a 9-h exposure. Cd exposure also induced oxidative stress, yet no inhibition of catalase gene activity was observed. Whole mount in situ hybridization revealed a wide distribution of msh6 mRNA in the head regions of 10 hpf embryos and pretreatment of embryos with antioxidants butylhydroxytoluene (BHT), D-mannitol or N-acetylcysteine (NAC) at 1-10 {mu}M restored Cd-suppressed msh6 expression. QPCR confirmed the protective effects of antioxidants on Cd-suppressed msh2/msh6 mRNA production. Down-regulated MSH gene activities reaching about 50% of control were also induced in embryos exposed to paraquat, a reactive oxygen species (ROS)-generating herbicide, or hydrogen peroxide at 200 {mu}M. Hence, Cd at sublethal levels down-regulates msh2/msh6 expression primarily via ROS as signaling molecules. The transcriptional activation of human msh6 is known to be fully dependent on the specificity factor 1 (Sp1). Cd failed to inhibit the DNA binding activity of zebrafish Sp1 unless at lethal concentrations based on band shift assay, therefore

  3. A Smoking Gun in the Carina Nebula (United States)

    Hamaguchi, Kenji; Corcoran, Michael F.; Ezoe, Yuichiro; Townsley, Leisa; Broos, Patrick; Gruendl, Robert; Vaidya, Kaushar; White, Stephen M.; Petre, Rob; Chu, You-Hua


    The Carina Nebula is one of thc youngest, most active sites of massive star formation in our Galaxy. In this nebula, we have discovered a bright X-ray source that has persisted for approx.30 years. The soft X-ray spectrum. consistent with kT approx.130 eV blackbody radiation with mild extinction, and no counterpart in the near- and mid-infrared wavelengths indicate that it is a, approx. 10(exp 6)-year-old neutron star housed in the Carina Nebula. Current star formation theory does not suggest that the progenitor of the neutron star and massive stars in the Carina Nebula, in particular (eta)Car, are coeval. This result demonstrates that the Carina Nebula experienced at least two major episodes of massive star formation. The neutron star would be responsible for remnants of high energy activity seen in multiple wavelengths.

  4. The Making of a Pre-Planetary Nebula (United States)

    Kohler, Susanna


    -planetary nebula, OH231, which lies 4,200 light-years away and is about 1.4 light-years long. This is a well studied nebula, so the team had many observations that their model needed to successfully replicate: the nebulas shapes, dimensions, overall geometry, locations of shocks, timescales, and even velocity gradients are known.The authors model included mass injection from the central source into the ambient gas in three different ways:clumps: spherical knots injected all at once,cylindrical jets: thin outflows with parallel streamlines, andsprays: conical outflows with diverging streamlines.Explanation from a Champagne BottlePanel A: best-fitting simulations of OH231 200, 400, and 800 yr after the clump and spray are launched. Panel B: example from the same family of solutions, in which the mass is reduced by a factor of 10. Click for a closer look. [Balick et al. 2017]Balick and collaborators found that by injecting the mass in these three ways with a specific order and spacing, they were able to find a family of solutions that very well replicated observations of OH231. In the best-fitting model, combinations of pairs of clumps are embedded within sprays of brief duration and launched into static ancient AGB winds. The authors compare the setup to the ejection of the cork and the spray of high-pressure fluid when a bottle of champagne is opened.These simulations successfully map out all but perhaps the first century of the nebulas evolution and give us some of the best insight yet into how these short-lived objects are formed. The authors are now working to reproduce these simulations for other pre-planetary nebulae, with the goal of piecing together common attributes of their ejection histories.CitationBruce Balick et al 2017 ApJ 843 108. doi:10.3847/1538-4357/aa77f0

  5. No association between MUTYH and MSH6 germline mutations in 64 HNPCC patients. (United States)

    Steinke, Verena; Rahner, Nils; Morak, Monika; Keller, Gisela; Schackert, Hans K; Görgens, Heike; Schmiegel, Wolff; Royer-Pokora, Brigitte; Dietmaier, Wolfgang; Kloor, Matthias; Engel, Christoph; Propping, Peter; Aretz, Stefan


    Hereditary non-polyposis colorectal cancer (HNPCC) is an autosomal dominant tumour predisposition syndrome caused by germline mutations in mismatch repair (MMR) genes. In contrast to MLH1 and MSH2, germline mutations in MSH6 are associated with a milder and particularly variable phenotype. Based on the reported interaction of the MMR complex and the base excision repair protein MUTYH, it was hypothesised that MUTYH mutations serve as phenotypical modifiers in HNPCC families. Recently, a significantly higher frequency of heterozygosity for MUTYH mutations among MSH6 mutation carriers was reported. We examined 64 MSH6 mutation carriers (42 truncating mutations, 19 missense mutations and 3 silent mutations) of the German HNPCC Consortium for MUTYH mutations by sequencing the whole coding region of the gene. Monoallelic MUTYH mutations were identified in 2 of the 64 patients (3.1%), no biallelic MUTYH mutation carrier was found. The frequency of MUTYH mutations was not significantly higher than that in healthy controls, neither in the whole patient group (P=0.30) nor in different subgroups regarding mutation type. Our results do not support the association between MSH6 mutations and heterozygosity for MUTYH mutations.

  6. alpha-MSH activates immediate defense responses to UV-induced oxidative stress in human melanocytes. (United States)

    Song, Xiuzu; Mosby, Nicole; Yang, Jennifer; Xu, Aie; Abdel-Malek, Zalfa; Kadekaro, Ana Luisa


    Exposure of cultured human melanocytes to ultraviolet radiation (UV) results in DNA damage. In melanoma, UV-signature mutations resulting from unrepaired photoproducts are rare, suggesting the possible involvement of oxidative DNA damage in melanocyte malignant transformation. Here we present data demonstrating immediate dose-dependent generation of hydrogen peroxide in UV-irradiated melanocytes, which correlated directly with a decrease in catalase activity. Pretreatment of melanocytes with alpha-melanocortin (alpha-MSH) reduced the UV-induced generation of 7,8-dihydro-8-oxyguanine (8-oxodG), a major form of oxidative DNA damage. Pretreatment with alpha-MSH also increased the protein levels of catalase and ferritin. The effect of alpha-MSH on 8-oxodG induction was mediated by activation of the melanocortin 1 receptor (MC1R), as it was absent in melanocytes expressing loss-of-function MC1R, and blocked by concomitant treatment with an analog of agouti signaling protein (ASIP), ASIP-YY. This study provides unequivocal evidence for induction of oxidative DNA damage by UV in human melanocytes and reduction of this damage by alpha-MSH. Our data unravel some mechanisms by which alpha-MSH protects melanocytes from oxidative DNA damage, which partially explain the strong association of loss-of-function MC1R with melanoma.

  7. Gene expression of the mismatch repair gene MSH2 in primary colorectal cancer

    DEFF Research Database (Denmark)

    Jensen, Lars Henrik; Kuramochi, Hidekazu; Crüger, Dorthe Gylling


    Microsatellite instability (MSI) is caused by defective mismatch repair (MMR) and is one of the very few molecular markers with proven clinical importance in colorectal cancer with respect to heredity, prognosis, and treatment effect. The gene expression of the MMR gene MSH2 may be a quantitative...... marker for the level of MMR and a potential molecular marker with clinical relevance. The aim was to investigate the gene expression of MSH2 in primary operable colorectal cancer in correlation with MSI, protein expression, and promoter hypermethylation. In a cohort of 210 patients, the primary tumor...... and lymphnode metastases were analyzed with immunohistochemistry, methylation and MSI analyses, and quantitative polymerase chain reaction (PCR). The median gene expression of MSH2 was 1.00 (range 0.16-11.2, quartiles 0.70-1.51) and there was good agreement between the gene expression in primary tumor and lymph...

  8. Synchronous lung tumours in a patient with metachronous colorectal carcinoma and a germline MSH2 mutation.

    LENUS (Irish Health Repository)

    Canney, A


    Mutations of DNA mismatch repair genes are characterised by microsatellite instability and are implicated in carcinogenesis. This mutation susceptible phenotype has been extensively studied in patients with hereditary non-polyposis colon carcinoma, but little is known of the contribution of such mutations in other tumour types, particularly non-small-cell lung carcinoma. This report describes the occurrence of two synchronous lung tumours, one mimicking a metastatic colon carcinoma, in a male patient with a history of metachronous colonic carcinoma. Immunohistochemistry supported a pulmonary origin for both lesions. Mismatch repair protein immunohistochemistry showed loss of MSH2 and MSH6 expression in both colonic tumours and in one lung tumour showing enteric differentiation. Subsequent mutational analysis demonstrated a deleterious germline mutation of the MSH2 mismatch repair gene. The significance of these findings and the practical diagnostic difficulties encountered in this case are discussed.

  9. NIF Discovery Science Eagle Nebula (United States)

    Kane, Jave; Martinez, David; Pound, Marc; Heeter, Robert; Casner, Alexis; Villette, Bruno; Mancini, Roberto


    The University of Maryland and and LLNL are investigating the origin and dynamics of the famous Pillars of the Eagle Nebula and similar parsec-scale structures at the boundaries of HII regions in molecular hydrogen clouds. The National Ignition Facility (NIF) Discovery Science program Eagle Nebula has performed NIF shots to study models of pillar formation. The shots feature a new long-duration x-ray source, in which multiple hohlraums mimicking a cluster of stars are driven with UV light in series for 10 to 15 ns each to create a 30 to 60 ns output x-ray pulse. The source generates deeply nonlinear hydrodynamics in the Eagle science package, a structure of dense plastic and foam mocking up a molecular cloud containing a dense core. Omega EP and NIF shots have validated the source concept, showing that earlier hohlraums do not compromise later ones by preheat or by ejecting ablated plumes that deflect later beams. The NIF shots generated radiographs of shadowing-model pillars, and also showed evidence that cometary structures can be generated. The velocity and column density profiles of the NIF shadowing and cometary pillars have been compared with observations of the Eagle Pillars made at the millimeter-wave BIMA and CARMA observatories. Prepared by LLNL under Contract DE-AC52-07NA27344.

  10. Molecular studies of Planetary Nebulae (United States)

    Zhang, Yong


    Circumstellar envelopes (CEs) around evolved stars are an active site for the production of molecules. After evolving through the Asymptotic Giant Branch (AGB), proto-planetary nebula (PPN), to planetary nebula (PN) phases, CEs ultimately merge with the interstellar medium (ISM). The study of molecules in PNe, therefore, is essential to understanding the transition from stellar to interstellar materials. So far, over 20 molecular species have been discovered in PNe. The molecular composition of PNe is rather different from those of AGB and PPNe, suggesting that the molecules synthesized in PN progenitors have been heavily processed by strong ultraviolet radiation from the central star. Intriguingly, fullerenes and complex organic compounds having aromatic and aliphatic structures can be rapidly formed and largely survive during the PPN/PN evolution. The similar molecular compositions in PNe and diffuse clouds as well as the detection of C60 + in the ISM reinforce the view that the mass-loss from PNe can significantly enrich the ISM with molecular species, some of which may be responsible for the diffuse interstellar bands. In this contribution, I briefly summarize some recent observations of molecules in PNe, with emphasis on their implications on circumstellar chemistry.

  11. NIF Discovery Science Eagle Nebula (United States)

    Kane, Jave; Martinez, David; Pound, Marc; Heeter, Robert; Huntington, Channing; Casner, Alexis; Villette, Bruno; Mancini, Roberto


    For almost 20 years a team of astronomers, theorists and experimentalists have investigated the creation of the famous Pillars of the Eagle Nebula and similar parsec-scale structures at the boundaries of HII regions in molecular hydrogen clouds, using a combination of astronomical observations, astrophysical simulations, and recently, scaled laboratory experiments. Eagle Nebula, one of the National Ignition Facility (NIF) Discovery Science programs, has completed four NIF shots to study the dense `shadowing' model of pillar formation, and been awarded more shots to study the `cometary' model. These experiments require a long-duration drive, 30 ns or longer, to generate deeply nonlinear ablative hydrodynamics. A novel x-ray source featuring multiple UV-driven hohlraums driven is used. The source directionally illuminates a science package, mimicking a cluster of stars. The first four NIF shots generated radiographs of shadowing-model pillars, and suggested that cometary structures can be generated. The velocity and column density profiles of the NIF shadowing and cometary pillars have been compared with observations of the Eagle Pillars made at millimeter observatories, and indicate cometary growth is key to matching observations. Supported in part by a Grant from the DOE OFES HEDLP program. Prepared by LLNL under Contract DE-AC52-07NA27344.

  12. Identification of MSH2 inversion of exons 1-7 in clinical evaluation of families with suspected Lynch syndrome. (United States)

    Mork, Maureen E; Rodriguez, Andrea; Taggart, Melissa W; Rodriguez-Bigas, Miguel A; Lynch, Patrick M; Bannon, Sarah A; You, Y Nancy; Vilar, Eduardo


    Traditional germline sequencing and deletion/duplication analysis does not detect Lynch syndrome-causing mutations in all individuals whose colorectal or endometrial tumors demonstrate mismatch repair (MMR) deficiency. Unique inversions and other rearrangements of the MMR genes have been reported in families with Lynch syndrome. In 2014, a recurrent inversion of MSH2 exons 1-7 was identified in five families suspected to have Lynch syndrome. We aimed to describe our clinical experience in identifying families with this specific inversion. Four probands whose Lynch syndrome-associated tumors demonstrated absence of MSH2/MSH6 staining and who had negative MMR germline testing were evaluated for the MSH2 inversion of exons 1-7, offered during initial genetic workup or upon routine clinical follow-up. All four probands tested positive for the MSH2 inversion. Proband cancer diagnoses included colon and endometrial adenocarcinoma and sebaceous adenoma. A variety of Lynch syndrome-associated cancers were reported in the family histories, although only one family met Amsterdam II criteria. Thirteen at-risk relatives underwent predictive testing. MSH2 inversion of exons 1-7 was found in four probands previously suspected to have Lynch syndrome based on family history and tumor testing. This testing should be offered routinely to patients with tumors demonstrating loss of MSH2/MSH6 staining.

  13. Intron splice acceptor site polymorphism in the hMSH2 gene in sporadic and familial colorectal cancer (United States)

    Palicio, M; Blanco, I; Tórtola, S; González, I; Marcuello, E; Brunet, J; Lluis, F; González-Aguilera, J J; Peinado, M A; Capella, G


    A polymorphism in hMSH2 gene has been associated with an increased susceptibility to develop colorectal cancer (CRC). Here we show that it is a genetic risk factor for CRC in the Spanish population. However, its presence does not apparently affect hMSH2 function. © 2000 Cancer Research Campaign PMID:10682661

  14. A homozygous MSH6 mutation in a child with café-au-lait spots, oligodendroglioma and rectal cancer. (United States)

    Menko, Fred H; Kaspers, Gertjan L; Meijer, Gerrit A; Claes, Kathleen; van Hagen, Johanna M; Gille, Johan J P


    Hereditary nonpolyposis colorectal cancer (HNPCC) is an autosomal dominant condition due to heterozygous germline mutations in DNA mismatch repair genes, in particular MLH1, MSH2 and MSH6. Recently, a syndrome was recognized in which children develop haematological malignancies, solid tumours and signs of neurofibromatosis type 1 due to bi-allelic MMR gene mutations in MLH1, MSH2 and PMS2. Here we describe the child of healthy consanguineous parents who had café-au-lait spots, oligodendroglioma, and rectal cancer. The patient was homozygous for the MSH6 mutation c.3386_3388delGTG in exon 5 which has a predicted pathogenic effect. Germline NF1 gene mutation testing was negative. The rectal tumour showed microsatellite instability and absence of MSH6 staining, whereas the brain tumour was MSI stable and showed normal immunohistochemical expression of MSH6. Apparently, not only MLH1, MSH2 and PMS2, but also MSH6 is involved in the syndrome of childhood cancer and signs of neurofibromatosis type 1.

  15. The effects of acute and chronic alpha melanocyte stimulating hormone (alphaMSH) on cardiovascular dynamics in conscious rats. (United States)

    Hill, Crystal; Dunbar, Joseph C


    Alpha melanocyte stimulating hormone (alphaMSH) has been demonstrated to have regulatory functions in the periphery and central nervous system (CNS). alphaMSH plays a central role in the regulation of metabolic balance such as decreasing food intake, increasing sympathetic outflow and hypothalamic/pituitary function. Our laboratory has investigated the actions of alphaMSH on sympathetic and cardiovascular dynamics using anesthetized animals. In this study we determined both the acute and chronic effects of alphaMSH on cardiovascular and metabolic dynamics in conscious unrestrained rats. Animals were each implanted with a radio-telemetry transmitter for recording of cardiovascular parameters and subsequently instrumented with intracerebroventricular (ICV) cannulas. The acute ICV administration of alphaMSH significantly increased the mean arterial pressure (MAP) and heart rate (HR) when compared to artificial cerebrospinal fluid (ACSF) controls. On the other hand chronic alphaMSH infusion resulted in an initial increase in MAP and HR lasting for 2 days followed by a decrease in MAP. Chronic alphaMSH administration decreased physical activity and food intake but not weight gain. We conclude that in the conscious unrestrained animal the acute administration of alphaMSH increased MAP and HR, however, chronic infusion is associated with decreased MAP, physical activity and food intake.

  16. Processing NASA Earth Science Data on Nebula Cloud (United States)

    Chen, Aijun; Pham, Long; Kempler, Steven


    Three applications were successfully migrated to Nebula, including S4PM, AIRS L1/L2 algorithms, and Giovanni MAPSS. Nebula has some advantages compared with local machines (e.g. performance, cost, scalability, bundling, etc.). Nebula still faces some challenges (e.g. stability, object storage, networking, etc.). Migrating applications to Nebula is feasible but time consuming. Lessons learned from our Nebula experience will benefit future Cloud Computing efforts at GES DISC.

  17. Identification of a new genetic marker in Mycoplasma synoviae vaccine strain MS-H and development of a strategy using polymerase chain reaction and high-resolution melting curve analysis for differentiating MS-H from field strains. (United States)

    Zhu, Ling; Konsak, Barbara M; Olaogun, Olusola M; Agnew-Crumptona, Rebecca; Kanci, Anna; Marenda, Marc S; Browning, Glenn F; Noormohammadi, Amir H


    Mycoplasma synoviae (MS) is an economically important avian pathogen worldwide, causing subclinical respiratory tract infection and infectious synovitis in chickens and turkeys. A temperature-sensitive (ts(+)) live attenuated vaccine MS-H, derived from the Australian field strain 86079/7NS, is now widely used in many countries to control the disease induced by MS. Differentiation of MS-H vaccine from field strains is crucial for monitoring vaccination programs in commercial poultry. Comparison of genomic sequences of MS-H and its parent strain revealed an adenine deletion at nucleotide position 468 of the MS-H oppF-1 gene. This mutation was shown to be unique to MS-H in further comparative analyses of oppF-1 genes of MS-H re-isolates and field strains from Australia and other countries. Based on this single nucleotide, a combination of nested PCR and high-resolution melting (HRM) curve analysis was used to evaluate its potential for use in differentiation of MS-H from field strains. The mean genotype confidence percentages of 99.27 and 48.20 for MS-H and field strains, respectively, demonstrated the high discriminative power of the newly developed assay (oppF PCR-HRM). A set of 13 tracheal swab samples collected from MS-H vaccinated specific pathogen free birds and commercial chicken flocks infected with MS were tested using the oppF PCR-HRM test and results were totally consistent with those obtained using vlhA genotyping. The nested-PCR HRM method established in this study proved to be a rapid, simple and cost effective tool for discriminating the MS-H vaccine strain from Australian and international strains in pure cultures and on tracheal swabs. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Major contribution from recurrent alterations and MSH6 mutations in the Danish Lynch syndrome population

    DEFF Research Database (Denmark)

    Nilbert, Mef; Wikman, Friedrik P; Hansen, Thomas V O


    An increasing number of mismatch-repair (MMR) gene mutations have been identified in hereditary nonpolyposis colorectal cancer (HNPCC) or Lynch syndrome. This study presents the population-based Danish MMR gene mutation profile, which contains 138 different MMR gene alterations. Among these, 88......+2_1667_+8TAAATCAdelinsATTT was identified in 14/58 (24%) MLH1 mutant families. The Danish Lynch syndrome population thus demonstrates that MSH6 mutations and recurrent/founder mutations have a larger contribution than previously recognized, which implies that the MSH6 gene should be included in routine diagnostics...

  19. Control of drying shrinkage in magnesium silicate hydrate (M-S-H) gel mortars


    Zhang, T; Liang, X; Li, C; Lorin, M; Li, Y; Vandeperre, LJM; Cheeseman, CR


    ? 2016 Elsevier Ltd. All rights reserved.Magnesium silicate hydrate (M-S-H) gel can be formed by the reaction of MgO with amorphous silica in the presence of sodium hexametaphosphate (Na-HMP). Typical pastes contain 40% MgO and 60% SF and have a w/c ratio of 0.5, but these exhibit shrinkage cracking on drying. The shrinkage characteristics of M-S-H mortar samples containing different additions of sand have been studied using dilatometry. The drying shrinkage was found to decrease with increas...



    In its first glimpse of the heavens following the successful December 1999 servicing mission, NASA's Hubble Space Telescope has captured a majestic view of a planetary nebula, the glowing remains of a dying, Sun-like star. This stellar relic, first spied by William Herschel in 1787, is nicknamed the 'Eskimo' Nebula (NGC 2392) because, when viewed through ground-based telescopes, it resembles a face surrounded by a fur parka. In this Hubble telescope image, the 'fur parka' is really a disk of material embellished with a ring of comet-shaped objects, with their tails streaming away from the central, dying star. The Eskimo's 'face' also contains some fascinating details. Although this bright central region resembles a ball of twine, it is, in reality, a bubble of material being blown into space by the central star's intense 'wind' of high-speed material. The planetary nebula began forming about 10,000 years ago, when the dying star began flinging material into space. The nebula is composed of two elliptically shaped lobes of matter streaming above and below the dying star. In this photo, one bubble lies in front of the other, obscuring part of the second lobe. Scientists believe that a ring of dense material around the star's equator, ejected during its red giant phase, created the nebula's shape. This dense waist of material is plodding along at 72,000 miles per hour (115,000 kilometers per hour), preventing high-velocity stellar winds from pushing matter along the equator. Instead, the 900,000-mile-per-hour (1.5-million-kilometer-per-hour) winds are sweeping the material above and below the star, creating the elongated bubbles. The bubbles are not smooth like balloons but have filaments of denser matter. Each bubble is about 1 light-year long and about half a light-year wide. Scientists are still puzzled about the origin of the comet-shaped features in the 'parka.' One possible explanation is that these objects formed from a collision of slow- and fast-moving gases

  1. A Starfish Preplanetary Nebula: IRAS 19024+0044 (United States)

    Sahai, Raghvendra; Sanchez Contreras, Carmen; Morris, Mark


    Using the Hubble Space Telescope, we have imaged the OH/IR star IRAS 19024+0044 (I19024) at 0.6, 0.8, 1.1, and 1.6 micrometers, as part of our surveys of candidate preplanetary nebulae. The images show a multipolar nebula of size approximately equal to 3.'7 2.'3, with at least six elongated lobes emanating from the center of the nebula. Two of the lobes show limb-brightened tips having point-symmetric structure with respect to the expected location of the central star. The central region shows two dark bands southwest and northeast of a central shallow maximum that may be either two inclined dusty toroidal structures or the dense parts of a single wide, inhomogeneous, toroid. Avery faint, surface brightness-limited, diffuse halo surrounds the lobes. Long-slit/echelle optical spectroscopy obtained at the Mount Palomar and Keck observatories shows a spatially compact source of H(alpha) emission; the H(alpha) line shows a strong, narrow, central core with very broad (+/-1000 km/sec), weak wings, and a narrower blueshifted absorption feature signifying the presence of an approximately 100 km/sec(exp -1) outflow. The spectrum is characterized by a strong, relatively featureless, continuum and lacks the strong forbidden emission lines characteristic of planetary nebulae, confirming that IRAS 19024 is a preplanetary nebula; the spectral type for the central star, although uncertain, is most likely early G. Interferometric observations of the CO J = 1 -0 line emission with the Owens Valley Radio Interferometer show a marginally resolved molecular envelope (size 5.'5 x 4.'4) with an expansion velocity of 13 km/sec (exp -1), resulting from the asymptotic giant branch (AGB) progenitor's dense, slow wind. We derive a kinematic distance of 3.5 kpc to I19024, based on its radial velocity. The bolometric flux is 7:3 x 10(exp -9) erg s(exp -1) cm(exp -2), and the luminosity 2850 L. The relatively low luminosity of I19024, in comparison with stellar evolutionary models, indicates

  2. Origin of the Napoleon's hat nebula around SN1987A and implications for the progenitor (United States)

    Podsiadlowski, Ph.; Fabian, A. C.; Stevens, I. R.


    A simple geometrical model for the emission nebula around SN1987A, whose morphology has been likened to Napoleon's hat, is presented. The model consists of a ring and a truncated double cone. When the effects of light travel time are included, the model reproduces the important topological structures of the nebula and makes detailed quantitative predictions for its future appearance. In particular, the hat-shaped northern rim is simply explained as the interaction of the light front with the northern cone. To explain the origin of the double cone, it is argued that the progenitor of SN1987A was in a binary system: its strong wind, colliding with a weaker wind from the companion star, created an asymptotic shock surface that was spread out into the required geometry by the rotation of the binary.

  3. LMC X-1: A New Spectral Analysis of the O-star in the Binary and Surrounding Nebula (United States)

    Hyde, E. A.; Russell, D. M.; Ritter, A.; Filipović, M. D.; Kaper, L.; Grieve, K.; O'Brien, A. N.


    We provide new observations of the LMC X-1 O star and its extended nebula structure using spectroscopic data from VLT/UVES as well as Hα imaging from the Wide Field Imager on the Max Planck Gesellschaft/European Southern Observatory 2.2 m telescope and ATCA imaging of the 2.1 GHz radio continuum. This nebula is one of the few known to be energized by an X-ray binary. We use a new spectrum extraction technique that is superior to other methods used to obtain both radial velocities and fluxes. This provides an updated spatial velocity of ≃ 21.0 +/- 4.8 km s-1 for the O star. The slit encompasses both the photo-ionized and shock-ionized regions of the nebula. The imaging shows a clear arc-like structure reminiscent of a wind bow shock in between the ionization cone and shock-ionized nebula. The observed structure can be fit well by the parabolic shape of a wind bow shock. If an interpretation of a wind bow shock system is valid, we investigate the N159-O1 star cluster as a potential parent of the system, suggesting a progenitor mass of ˜60 M ⊙ for the black hole. We further note that the radio emission could be non-thermal emission from the wind bow shock, or synchrotron emission associated with the jet-inflated nebula. For both wind- and jet-powered origins, this would represent one of the first radio detections of such a structure.

  4. Nebulae and how to observe them

    CERN Document Server

    Coe, Steven


    This "Astronomers' Observing Guides" are designed for practical amateur astronomers who not only want to observe, but want to know the details of exactly what they are looking at. Nebulae are the places where the stars are born. For amateur astronomers, the many different kinds of nebulae vary from "easy" targets that can be seen with modest equipment under mediocre skies, to "challenging" objects that require experienced observers, large telescopes and excellent seeing. The concept of the book - and of the series - is to present an up-to-date detailed description and categorisation (part one); and then (part two) to consider how best to successfully observe and record the large range of astronomical objects that fall under the general heading of "nebulae". "Nebulae, and How to Observe Them" is a mine of information for all levels of amateur observers, from the beginner to the experienced.

  5. Evolutionary status of the Of?p star HD 148937 and of its surrounding nebula NGC 6164/5 (United States)

    Mahy, L.; Hutsemékers, D.; Nazé, Y.; Royer, P.; Lebouteiller, V.; Waelkens, C.


    Aims: The magnetic star HD 148937 is the only Galactic Of?p star surrounded by a nebula. The structure of this nebula is particularly complex and is composed, from the center out outwards, of a close bipolar ejecta nebula (NGC 6164/5), an ellipsoidal wind-blown shell, and a spherically symmetric Strömgren sphere. The exact formation process of this nebula and its precise relation to the star's evolution remain unknown. Methods: We analyzed infrared Spitzer IRS and far-infrared Herschel/PACS observations of the NGC 6164/5 nebula. The Herschel imaging allowed us to constrain the global morphology of the nebula. We also combined the infrared spectra with optical spectra of the central star to constrain its evolutionary status. We used these data to derive the abundances in the ejected material. To relate this information to the evolutionary status of the star, we also determined the fundamental parameters of HD 148937 using the CMFGEN atmosphere code. Results: The Hα image displays a bipolar or "8"-shaped ionized nebula, whilst the infrared images show dust to be more concentrated around the central object. We determine nebular abundance ratios of N/O = 1.06 close to the star, and N/O = 1.54 in the bright lobe constituting NGC 6164. Interestingly, the parts of the nebula located further from HD 148937 appear more enriched in stellar material than the part located closer to the star. Evolutionary tracks suggest that these ejecta have occured 1.2-1.3 and 0.6 Myr ago, respectively. In addition, we derive abundances of argon for the nebula compatible with the solar values and we find a depletion of neon and sulfur. The combined analyses of the known kinematics and of the new abundances of the nebula suggest either a helical morphology for the nebula, possibly linked to the magnetic geometry, or the occurrence of a binary merger. Herschel is an ESA space observatory with science instruments provided by European-led Principal Investigator consortia and with important

  6. Functional analysis of HNPCC-related missense mutations in MSH2

    DEFF Research Database (Denmark)

    Lützen, Anne; de Wind, Niels; Georgijevic, Dubravka


    Hereditary nonpolyposis colorectal cancer (HNPCC) is associated with germline mutations in the human DNA mismatch repair (MMR) genes, most frequently MSH2 and MLH1. The majority of HNPCC mutations cause truncations and thus loss of function of the affected polypeptide. However, a significant...

  7. Loss of hMSH2 gene expression correlates with improved survival in ...

    Indian Academy of Sciences (India)

    Introduction. A mismatch repair gene hMSH2 (human mutS homolog 2) is involved in the correction of mispairing during replication, and its mutation is associated with both microsatellite insta- bility and hereditary colorectal cancer. We have also evalu- ated its involvement in sporadic colorectal cancer tumorige- nesis.

  8. Suspected Lynch syndrome associated MSH6 variants: A functional assay to determine their pathogenicity.

    Directory of Open Access Journals (Sweden)

    Hellen Houlleberghs


    Full Text Available Lynch syndrome (LS is a hereditary cancer predisposition caused by inactivating mutations in DNA mismatch repair (MMR genes. Mutations in the MSH6 DNA MMR gene account for approximately 18% of LS cases. Many LS-associated sequence variants are nonsense and frameshift mutations that clearly abrogate MMR activity. However, missense mutations whose functional implications are unclear are also frequently seen in suspected-LS patients. To conclusively diagnose LS and enroll patients in appropriate surveillance programs to reduce morbidity as well as mortality, the functional consequences of these variants of uncertain clinical significance (VUS must be defined. We present an oligonucleotide-directed mutagenesis screen for the identification of pathogenic MSH6 VUS. In the screen, the MSH6 variant of interest is introduced into mouse embryonic stem cells by site-directed mutagenesis. Subsequent selection for MMR-deficient cells using the DNA damaging agent 6-thioguanine (6TG allows the identification of MMR abrogating VUS because solely MMR-deficient cells survive 6TG exposure. We demonstrate the efficacy of the genetic screen, investigate the phenotype of 26 MSH6 VUS and compare our screening results to clinical data from suspected-LS patients carrying these variant alleles.

  9. The amphibian melanization inhibiting factor (MIF) blocks the alpha-MSH effect on mouse malignant melanocytes. (United States)

    López-Contreras, A M; Martínez-Liarte, J H; Solano, F; Samaraweera, P; Newton, J M; Bagnara, J T


    We have found that a melanization inhibitory factor (MIF) extracted from the ventral skin of Rana forreri has a slight inhibitory effect on the activity levels of tyrosinase and dopachrome tautomerase in B16/F10 and Cloudman S-91 murine melanoma cell lines. Furthermore, this factor appears to block the effects of alpha-MSH on these enzymatic activities. However, MIF treatment does not affect the melanogenic action of theophylline on the same cells, suggesting that MIF acts proximal to MSH-mediated cAMP formation, possibly by interaction with the MSH receptor. In this way, we show that this amphibian factor has biological activity on mammalian melanocytes. This suggests the existence of mammalian counterparts of amphibian MIF in the mouse integument that might regulate epidermal melanocytes. These peptides might be related to the agouti protein, as they share similar mechanisms of action. The interaction of different peptides with the MSH receptor would be a complex but general mechanism responsible for many mammalian coat color variants.

  10. Novel Mutations in MLH1 and MSH2 Genes in Mexican Patients with Lynch Syndrome

    Directory of Open Access Journals (Sweden)

    Jose Miguel Moreno-Ortiz


    Full Text Available Background. Lynch Syndrome (LS is characterized by germline mutations in the DNA mismatch repair (MMR genes MLH1, MSH2, MSH6, and PMS2. This syndrome is inherited in an autosomal dominant pattern and is characterized by early onset colorectal cancer (CRC and extracolonic tumors. The aim of this study was to identify mutations in MMR genes in three Mexican patients with LS. Methods. Immunohistochemical analysis was performed as a prescreening method to identify absent protein expression. PCR, Denaturing High Performance Liquid Chromatography (dHPLC, and Sanger sequencing complemented the analysis. Results. Two samples showed the absence of nuclear staining for MLH1 and one sample showed loss of nuclear staining for MSH2. The mutations found in MLH1 gene were c.2103+1G>C in intron 18 and compound heterozygous mutants c.1852_1854delAAG (p.K618del and c.1852_1853delinsGC (p.K618A in exon 16. In the MSH2 gene, we identified mutation c.638dupT (p.L213fs in exon 3. Conclusions. This is the first report of mutations in MMR genes in Mexican patients with LS and these appear to be novel.

  11. Muir-Torre Syndrome Presenting as Sebaceous Adenocarcinoma and Invasive MSH6-Positive Colorectal Adenocarcinoma

    Directory of Open Access Journals (Sweden)

    Sunil Tulpule


    Full Text Available Muir-Torre syndrome (MTS is a rare genodermatosis, diagnosed by the presence of sebaceous neoplasms along with an internal malignancy, most commonly colorectal carcinomas. MTS is most commonly caused by microsatellite instabilities of the hMLH1 and hMSH2 mismatch repair genes, and is rarely caused by mutations of the hMSH6 gene. We describe the case of a 56-year-old male who presented with an enlarging mass on his back as well as hematochezia. The back mass was excised, and pathology confirmed microsatellite instability in MSH2 and MSH6. Abdominal CT and colonoscopy confirmed the presence of synchronous masses in the cecum, ascending colon, and the transverse colon. He refused any further workup or treatment, only to return 8 months later complaining of hematochezia and discomfort due to an enlarging mass protruding from the rectum. After consenting to surgical intervention, he agreed to outpatient chemotherapy treatment. The presence of sebaceous neoplasms should raise suspicion for the possibility of an associated internal malignancy.

  12. Predictive value of MSH2 gene expression in colorectal cancer treated with capecitabine

    DEFF Research Database (Denmark)

    Jensen, Lars H; Danenberg, Kathleen D; Danenberg, Peter V


    PURPOSE: The objective of the present study was to evaluate the gene expression of the DNA mismatch repair gene MSH2 as a predictive marker in advanced colorectal cancer (CRC) treated with first-line capecitabine. PATIENTS AND METHODS: Microdissection of paraffin-embedded tumor tissue, RNA...

  13. Strong Winds over the Keel (United States)


    The latest ESO image reveals amazing detail in the intricate structures of one of the largest and brightest nebulae in the sky, the Carina Nebula (NGC 3372), where strong winds and powerful radiation from an armada of massive stars are creating havoc in the large cloud of dust and gas from which the stars were born. ESO PR Photo 05a/09 The Carina Nebula ESO PR Video 05a/09 Pan over the Carina Nebula ESO PR Video 05b/09 Carina Nebula Zoom-in The large and beautiful image displays the full variety of this impressive skyscape, spattered with clusters of young stars, large nebulae of dust and gas, dust pillars, globules, and adorned by one of the Universe's most impressive binary stars. It was produced by combining exposures through six different filters from the Wide Field Imager (WFI), attached to the 2.2 m ESO/MPG telescope at ESO's La Silla Observatory, in Chile. The Carina Nebula is located about 7500 light-years away in the constellation of the same name (Carina; the Keel). Spanning about 100 light-years, it is four times larger than the famous Orion Nebula and far brighter. It is an intensive star-forming region with dark lanes of cool dust splitting up the glowing nebula gas that surrounds its many clusters of stars. The glow of the Carina Nebula comes mainly from hot hydrogen basking in the strong radiation of monster baby stars. The interaction between the hydrogen and the ultraviolet light results in its characteristic red and purple colour. The immense nebula contains over a dozen stars with at least 50 to 100 times the mass of our Sun. Such stars have a very short lifespan, a few million years at most, the blink of an eye compared with the Sun's expected lifetime of ten billion years. One of the Universe's most impressive stars, Eta Carinae, is found in the nebula. It is one of the most massive stars in our Milky Way, over 100 times the mass of the Sun and about four million times brighter, making it the most luminous star known. Eta Carinae is highly

  14. Autoantibodies against α-MSH, ACTH, and LHRH in anorexia and bulimia nervosa patients (United States)

    Fetissov, Sergueï O.; Hallman, Jarmila; Oreland, Lars; af Klinteberg, Britt; Grenbäck, Eva; Hulting, Anna-Lena; Hökfelt, Tomas


    The hypothalamic arcuate nucleus is involved in the control of energy intake and expenditure and may participate in the pathogenesis of eating disorders such as anorexia nervosa (AN) and bulimia nervosa (BN). Two systems are of particular interest in this respect, synthesizing α-melanocyte-stimulating hormone (α-MSH) and synthesizing neuropeptide Y, respectively. We report here that 42 of 57 (74%) AN and/or BN patients studied had in their plasma Abs that bind to melanotropes and/or corticotropes in the rat pituitary. Among these sera, 8 were found to bind selectively to α-MSH-positive neurons and their hypothalamic and extrahypothalamic projections as revealed with immunostaining on rat brain sections. Adsorption of these sera with α-MSH peptide abolished this immunostaining. In the pituitary, the immunostaining was blocked by adsorption with α-MSH or adrenocorticotropic hormone. Additionally, 3 AN/BN sera bound to luteinizing hormone-releasing hormone (LHRH)-positive terminals in the rat median eminence, but only 2 of them were adsorbed with LHRH. In the control subjects, 2 of 13 sera (16%) displayed similar to AN/BN staining. These data provide evidence that a significant subpopulation of AN/BN patients have autoantibodies that bind to α-MSH or adrenocorticotropic hormone, a finding pointing also to involvement of the stress axis. It remains to be established whether these Abs interfere with normal signal transduction in the brain melanocortin circuitry/LHRH system and/or in other central and peripheral sites relevant to food intake regulation, to what extent such effects are related to and/or could be involved in the pathophysiology or clinical presentation of AN/BN, and to what extent increased stress is an important factor for production of these autoantibodies. PMID:12486250

  15. Photoionization modelling of planetary nebulae - II. Galactic bulge nebulae, a comparison with literature results

    NARCIS (Netherlands)

    van Hoof, PAM; Van de Steene, GC


    We have constructed photoionization models of five galactic bulge planetary nebulae using our automatic method, which enables a fully self-consistent determination of the physical parameters of a planetary nebula. The models are constrained using the spectrum, the IRAS and radio fluxes and the

  16. Functional analysis in mouse embryonic stem cells reveals wild-type activity for three MSH6 variants found in suspected Lynch syndrome patients.

    Directory of Open Access Journals (Sweden)

    Eva A L Wielders

    Full Text Available Lynch syndrome confers an increased risk to various types of cancer, in particular early onset colorectal and endometrial cancer. Mutations in mismatch repair (MMR genes underlie Lynch syndrome, with the majority of mutations found in MLH1 and MSH2. Mutations in MSH6 have also been found but these do not always cause a clear cancer predisposition phenotype and MSH6-defective tumors often do not show the standard characteristics of MMR deficiency, such as microsatellite instability. In particular, the consequences of MSH6 missense mutations are challenging to predict, which further complicates genetic counseling. We have previously developed a method for functional characterization of MSH2 missense mutations of unknown significance. This method is based on endogenous gene modification in mouse embryonic stem cells using oligonucleotide-directed gene targeting, followed by a series of functional assays addressing the MMR functions. Here we have adapted this method for the characterization of MSH6 missense mutations. We recreated three MSH6 variants found in suspected Lynch syndrome families, MSH6-P1087R, MSH6-R1095H and MSH6-L1354Q, and found all three to behave like wild type MSH6. Thus, despite suspicion for pathogenicity from clinical observations, our approach indicates these variants are not disease causing. This has important implications for counseling of mutation carriers.

  17. On dust irradiation in planetary nebulae in the context of survivability of ices (United States)

    Yeghikyan, Ararat


    A large number of molecules are observed in planetary nebulae, including simple and, - the most common (H2, CO and OH), more complex (H2O, SiO, HCN, HNC, HCO+), and even the polycyclic aromatic hydrocarbons and fullerenes containing a few dozen and more atoms. Water molecules are observed, as a rule, in the young objects, in the gas phase (water "fountains" and related water masers) and solid phase (emission of crystalline ice particles). On the other hand, the results of calculations by the Cloudy computer program, given in this paper, show that the abundance of water ice in planetary nebulae, other conditions being equal, depends on the ionization rate of hydrogen, which depends in turn on the flux of energetic particles (protons and alpha particles) in the range of MeV energies and higher. Calculated water ice column densities reach values of up to 1019 -1020 cm-2 at the usual average ISM H2 ionisation rate of 10-16s-1 and sharply decrease at rates that are a thousand times larger. The possibility of an increased flux of energetic particles in planetary nebulae under conditions of the standard interacting stellar winds scenario is discussed, and it is concluded that the flux may locally exceed by 1-3 orders of magnitude that of galactic cosmic rays. This may have important implications for the chemistry of complex compounds under conditions of planetary nebulae, in particular, for models of the origin of fullerenes.

  18. Discovery of powerful gamma-ray flares from the Crab Nebula. (United States)

    Tavani, M; Bulgarelli, A; Vittorini, V; Pellizzoni, A; Striani, E; Caraveo, P; Weisskopf, M C; Tennant, A; Pucella, G; Trois, A; Costa, E; Evangelista, Y; Pittori, C; Verrecchia, F; Del Monte, E; Campana, R; Pilia, M; De Luca, A; Donnarumma, I; Horns, D; Ferrigno, C; Heinke, C O; Trifoglio, M; Gianotti, F; Vercellone, S; Argan, A; Barbiellini, G; Cattaneo, P W; Chen, A W; Contessi, T; D'Ammando, F; DePris, G; Di Cocco, G; Di Persio, G; Feroci, M; Ferrari, A; Galli, M; Giuliani, A; Giusti, M; Labanti, C; Lapshov, I; Lazzarotto, F; Lipari, P; Longo, F; Fuschino, F; Marisaldi, M; Mereghetti, S; Morelli, E; Moretti, E; Morselli, A; Pacciani, L; Perotti, F; Piano, G; Picozza, P; Prest, M; Rapisarda, M; Rappoldi, A; Rubini, A; Sabatini, S; Soffitta, P; Vallazza, E; Zambra, A; Zanello, D; Lucarelli, F; Santolamazza, P; Giommi, P; Salotti, L; Bignami, G F


    The well-known Crab Nebula is at the center of the SN1054 supernova remnant. It consists of a rotationally powered pulsar interacting with a surrounding nebula through a relativistic particle wind. The emissions originating from the pulsar and nebula have been considered to be essentially stable. Here, we report the detection of strong gamma-ray (100 mega-electron volts to 10 giga-electron volts) flares observed by the AGILE satellite in September 2010 and October 2007. In both cases, the total gamma-ray flux increased by a factor of three compared with the non-flaring flux. The flare luminosity and short time scale favor an origin near the pulsar, and we discuss Chandra Observatory x-ray and Hubble Space Telescope optical follow-up observations of the nebula. Our observations challenge standard models of nebular emission and require power-law acceleration by shock-driven plasma wave turbulence within an approximately 1-day time scale.

  19. The Chandra planetary nebula survey (CHANPLANS). II. X-ray emission from compact planetary nebulae

    Energy Technology Data Exchange (ETDEWEB)

    Freeman, M.; Kastner, J. H. [Center for Imaging Science and Laboratory for Multiwavelength Astrophysics, Rochester Institute of Technology, 54 Lomb Memorial Drive, Rochester, NY 14623 (United States); Montez, R. Jr. [Department of Physics and Astronomy, Vanderbilt University, Nashville, TN (United States); Balick, B. [Department of Astronomy, University of Washington, Seattle, WA (United States); Frew, D. J.; De Marco, O.; Parker, Q. A. [Department of Physics and Astronomy and Macquarie Research Centre for Astronomy, Astrophysics and Astrophotonics, Macquarie University, Sydney, NSW 2109 (Australia); Jones, D. [Departamento de Física, Universidad de Atacama, Copayapu 485, Copiapó (Chile); Miszalski, B. [South African Astronomical Observatory, P.O. Box 9, Observatory, 7935 (South Africa); Sahai, R. [Jet Propulsion Laboratory, MS 183-900, California Institute of Technology, Pasadena, CA 91109 (United States); Blackman, E.; Frank, A. [Department of Physics and Astronomy, University of Rochester, Rochester, NY (United States); Chu, Y.-H. [Department of Astronomy, University of Illinois at Urbana-Champaign, Urbana, IL (United States); Guerrero, M. A. [Instituto de Astrofísica de Andalucía, IAA-CSIC, Glorieta de la Astronomía s/n, Granada, E-18008 (Spain); Lopez, J. A. [Instituto de Astronomía, Universidad Nacional Autonoma de Mexico, Campus Ensenada, Apdo. Postal 22860, Ensenada, B. C. (Mexico); Zijlstra, A. [School of Physics and Astronomy, University of Manchester, Manchester M13 9PL (United Kingdom); Bujarrabal, V. [Instituto de Astrofísica de Canarias, E-38200 La Laguna, Tenerife (Spain); Corradi, R. L. M. [Departamento de Astrofísica, Universidad de La Laguna, E-38206 La Laguna, Tenerife (Spain); Nordhaus, J. [NSF Astronomy and Astrophysics Fellow, Center for Computational Relativity and Gravitation, Rochester Institute of Technology, Rochester, NY 14623 (United States); and others


    We present results from the most recent set of observations obtained as part of the Chandra X-ray observatory Planetary Nebula Survey (CHANPLANS), the first comprehensive X-ray survey of planetary nebulae (PNe) in the solar neighborhood (i.e., within ∼1.5 kpc of the Sun). The survey is designed to place constraints on the frequency of appearance and range of X-ray spectral characteristics of X-ray-emitting PN central stars and the evolutionary timescales of wind-shock-heated bubbles within PNe. CHANPLANS began with a combined Cycle 12 and archive Chandra survey of 35 PNe. CHANPLANS continued via a Chandra Cycle 14 Large Program which targeted all (24) remaining known compact (R {sub neb} ≲ 0.4 pc), young PNe that lie within ∼1.5 kpc. Results from these Cycle 14 observations include first-time X-ray detections of hot bubbles within NGC 1501, 3918, 6153, and 6369, and point sources in HbDs 1, NGC 6337, and Sp 1. The addition of the Cycle 14 results brings the overall CHANPLANS diffuse X-ray detection rate to ∼27% and the point source detection rate to ∼36%. It has become clearer that diffuse X-ray emission is associated with young (≲ 5 × 10{sup 3} yr), and likewise compact (R {sub neb} ≲ 0.15 pc), PNe with closed structures and high central electron densities (n{sub e} ≳ 1000 cm{sup –3}), and is rarely associated with PNe that show H{sub 2} emission and/or pronounced butterfly structures. Hb 5 is one such exception of a PN with a butterfly structure that hosts diffuse X-ray emission. Additionally, two of the five new diffuse X-ray detections (NGC 1501 and NGC 6369) host [WR]-type central stars, supporting the hypothesis that PNe with central stars of [WR]-type are likely to display diffuse X-ray emission.

  20. Case Report of Menopausal Woman Diagnosed with Endometrial Cancer after Colon Cancer with Germline Mutation in MSH6 in Korea. (United States)

    Lee, Hyun Jung; Lee, Min Hee; Choi, Min Chul; Jung, Sang Geun; Joo, Won Duk; Kim, Tae Hoen; Lee, Chan; Jang, Ja-Hyun


    We present a case of an endometrial cancer patient with germline mutation in MutS homolog 6 (MSH6), associated with Lynch syndrome. A 60-year-old Korean woman had a personal history of colon cancer 23 years ago. She also had a family history of endometrial cancer and colon cancer of her sisters and brothers. Immunohistochemistry was negative for MutL homolog 1 (MLH1) and positive for MutS homolog 2 (MSH2). Based on these findings, she underwent genetic counseling and testing that revealed a frameshift germline mutation at MSH6 (c. 3261dupC).

  1. Functional characterization of rare missense mutations in MLH1 and MSH2 identified in Danish colorectal cancer patients

    DEFF Research Database (Denmark)

    Christensen, Lise Lotte; Kariola, Reetta; Korhonen, Mari K


    Recently, we have performed a population based study to analyse the frequency of colorectal cancer related MLH1 and MSH2 missense mutations in the Danish population. Half of the analyzed mutations were rare and most likely only present in the families where they were identified originally. Some...... of the missense mutations were located in conserved regions in the MLH1 and MSH2 proteins indicating a relation to disease development. In the present study, we functionally characterized 10 rare missense mutations in MLH1 and MSH2 identified in 13 Danish CRC families. To elucidate the pathogenicity...

  2. On continuum driven winds from rotating stars


    Shacham, Tomer; Shaviv, Nir J.


    We study the dynamics of continuum driven winds from rotating stars, and develop an approximate analytical model. We then discuss the evolution of stellar angular momentum, and show that just above the Eddington limit, the winds are sufficiently concentrated towards the poles to spin up the star. A twin-lobe structure of the ejected nebula is seen to be a generic consequence of critical rotation. We find that if the pressure in such stars is sufficiently dominated by radiation, an equatorial ...

  3. Photodestruction of PAHs in Planetary Nebulae (United States)

    Boechat-Roberty, H. M.; Neves, R.; Pilling, S.; de Souza G. G., B.; Lago, A.

    It is known that polycyclic aromatic hydrocarbons (PAHs) are mainly formed in the dust shells of late stages of AGB type carbon rich stars. After the ejection of H-rich envelope those stars become the proto-planetary nebulae (PPNs). The chemistry in PPNs has been strongly modified by the UV photons coming from the hot central star and by the X-rays associated with its high-velocity winds. Benzene (C6H6) and small PAHs like Anthracene (C14H10) were effectively detected in the PPNs CRL 618 (Cernicharo et al. 2001) and Red Rectangle (Vijh, Witt & Gordon 2004) respectively. The goal of this work is to experimentally study photoabsorption, photoionization and photodissociation processes of the benzene, biphenyl (C12H10), naphthalene (C10H8), phenanthrene (C14H10) and methyl-anthracene (C14H9(CH3)). The measurements were taken at the Brazilian Synchrotron Light Laboratory (LNLS), using soft X-ray and UV photons from a toroidal grating monochromator TGM beamline (12-310 eV). The experimental set-up consists of a high vacuum chamber with a Time-Of-Flight Mass Spectrometer (TOF-MS). Mass spectra were obtained using PhotoElectron PhotoIon Coincidence (PEPICO) technique. Kinetic energy distributions and abundances for each ionic fragment have been obtained from the analysis of the corresponding peak shapes in the mass spectra. Dissociative and non-dissociative photoionization cross sections for some molecules were also determined (see for example: Boechat-Roberty, Pilling & Santos 2005). We have observed that PAHs molecules are extreme resistant to UV photons, confirming that PAHs absorb the UV photons and after some internal energetic rearrangements, they can emit in the IR range. However, these molecules are destroyed by soft X-rays photons producing several ionic fragments, some of them with great kinetic energy. In the mass spectra of the Benzene and methyl-anthracene molecules, the observed ionic fragments C4H2+, C6H2+, C4HCH3 and C2HCH3, could correspond to the same

  4. Germline MSH6 mutations are more prevalent in endometrial cancer patient cohorts than hereditary non polyposis colorectal cancer cohorts. (United States)

    Devlin, Lisa A; Graham, Colin A; Price, John H; Morrison, Patrick J


    To determine and compare the prevalence of MSH6 (a mismatch repair gene) mutations in a cohort of families with Hereditary Non-Polyposis Colorectal Cancer (HNPCC), and in an unselected cohort of endometrial cancer patients (EC). Two patient cohorts participated in the study. A cohort of HNPCC families who were known to the Regional Medical Genetics department, and an unselected cohort of patients with a history of EC. All participants received genetic counselling on the implications of molecular testing, and blood was taken for DNA extraction with consent. All samples underwent sequencing and Multiple Ligation probe analysis (MLPA) for mutations in MSH6. DNA from one hundred and forty-three probands from HNPCC families and 125 patients with EC were included in the study. Molecular analysis of DNA in all participants from both cohorts for mutations in MSH6. Prevalence of pathogenic mutations in MSH6. A truncating mutation in MSH6 was identified in 3.8% (95% CI 1.0-9.5%) of patients in the endometrial cancer cohort, and 2.6% (95% CI 0.5-7.4%) of patients in the HNPCC cohort. A missense mutation was identified in 2.9% and 4.4% of the same cohorts respectively. No genomic rearrangements in MSH6 were identified. MSH6 mutations are more common in EC patients than HNPCC families. Genomic rearrangements do not contribute to a significant proportion of mutations in MSH6, but missense variants are relatively common and their pathogenicity can be uncertain. HNPCC families may be ascertained through an individual presenting with EC, and recognition of these families is important so that appropriate cancer surveillance can be put in place.

  5. Monitoring the Crab Nebula with LOFT (United States)

    Wilson-Hodge, Colleen A.


    From 2008-2010, the Crab Nebula was found to decline by 7% in the 15-50 keV band, consistently in Fermi GBM, INTEGRAL IBIS, SPI, and JEMX, RXTE PCA, and Swift BAT. From 2001-2010, the 15-50 keV flux from the Crab Nebula typically varied by about 3.5% per year. Analysis of RXTE PCA data suggests possible spectral variations correlated with the flux variations. I will present estimates of the LOFT sensitivity to these variations. Prior to 2001 and since 2010, the observed flux variations have been much smaller. Monitoring the Crab with the LOFT WFM and LAD will provide precise measurements of flux variations in the Crab Nebula if it undergoes a similarly active episode.

  6. Planetary Nebulae Beyond the Milky Way

    CERN Document Server

    Stanghellini, L; Douglas, N. G; Proceedings of the ESO Workshop held at Garching, Germany, 19-21 May, 2004


    In the last decade extra-galactic planetary nebulae (PNe) have gained increasing importance. Improved observational capabilities have allowed fainter and fainter PNe to be studied in galaxies well beyond the Milky Way. Planetary nebulae can be detected to at least 30Mpc. They are found in galaxies of all types and also between the galaxies in nearby galaxy clusters. They are valuable as probes, both for providing the velocity of their host stars and also the evolutionary status and relation to the stellar population from which they formed. This book contains the proceedings of a workshop held at ESO headquarters in Garching in 2004, the first meeting devoted entirely to Extra-galactic Planetary Nebulae. A wide range of topics is covered, from stellar and nebular astrophysics to galactic dynamics and galaxy clusters, making this volume a unique and timely reference of broad astrophysical interest.

  7. Hubble Space Telescope Image of Omega Nebula (United States)


    This sturning image, taken by the newly installed Advanced Camera for Surveys (ACS) aboard the Hubble Space Telescope (HST), is an image of the center of the Omega Nebula. It is a hotbed of newly born stars wrapped in colorful blankets of glowing gas and cradled in an enormous cold, dark hydrogen cloud. The region of nebula shown in this photograph is about 3,500 times wider than our solar system. The nebula, also called M17 and the Swan Nebula, resides 5,500 light-years away in the constellation Sagittarius. The Swan Nebula is illuminated by ultraviolet radiation from young, massive stars, located just beyond the upper-right corner of the image. The powerful radiation from these stars evaporates and erodes the dense cloud of cold gas within which the stars formed. The blistered walls of the hollow cloud shine primarily in the blue, green, and red light emitted by excited atoms of hydrogen, nitrogen, oxygen, and sulfur. Particularly striking is the rose-like feature, seen to the right of center, which glows in the red light emitted by hydrogen and sulfur. As the infant stars evaporate the surrounding cloud, they expose dense pockets of gas that may contain developing stars. One isolated pocket is seen at the center of the brightest region of the nebula. Other dense pockets of gas have formed the remarkable feature jutting inward from the left edge of the image. The color image is constructed from four separate images taken in these filters: blue, near infrared, hydrogen alpha, and doubly ionized oxygen. Credit: NASA, H. Ford (JHU), G. Illingworth (USCS/LO), M. Clampin (STScI), G. Hartig (STScI), the ACS Science Team, and ESA.

  8. Repair genes expression profile of MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers. (United States)

    Alves, Mônica Ghislaine Oliveira; Carta, Celina Faig Lima; de Barros, Patrícia Pimentel; Issa, Jaqueline Scholz; Nunes, Fábio Daumas; Almeida, Janete Dias


    The aim of this study was to evaluate the effect of chronic smoking on the expression profile of the repair genes MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers and never smokers. The sample consisted of thirty exfoliative cytology smears per group obtained from Smokers and Never Smokers. Total RNA was extracted and expression of the MLH1, MSH2 and ATM genes were evaluated by quantitative real-time and immunocytochemistry. The gene and protein expression data were correlated to the clinical data. Gene expression was analyzed statistically using the Student t-test and Pearson's correlation coefficient, with pATM genes were downregulated in the smoking group compared to the control with significant values for MLH1 (p=0.006), MSH2 (p=0.0001) and ATM (p=0.0001). Immunocytochemical staining for anti-MLH1, anti-MSH2 and anti-ATM was negative in Never Smokers; in Smokers it was rarely positive. No significant correlation was observed among the expression of MLH1, MSH2, ATM and age, number of cigarettes consumed per day, time of smoking during life, smoking history or levels of CO in expired air. The expression of genes and proteins related to DNA repair mechanism MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers was reduced. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Large-scale bioreactor production of the herbicide-degrading Aminobacter sp. strain MSH1

    DEFF Research Database (Denmark)

    Schultz-Jensen, Nadja; Knudsen, Berith Elkær; Frkova, Zuzana


    The Aminobacter sp. strain MSH1 has potential for pesticide bioremediation because it degrades the herbicide metabolite 2,6-dichlorobenzamide (BAM). Production of the BAM-degrading bacterium using aerobic bioreactor fermentation was investigated. A mineral salt medium limited for carbon and with ......, large-scale production of herbicide-degrading Aminobacter was possible, bringing the use of this bacterium in bioaugmentation field remediation closer to reality....

  10. Structural and Enzymatic Analysis of MshA from Corynebacterium glutamicum

    Energy Technology Data Exchange (ETDEWEB)

    Vetting,M.; Frantom, P.; Blanchard, J.


    The glycosyltransferase termed MshA catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to 1-l-myo-inositol-1-phosphate in the first committed step of mycothiol biosynthesis. The structure of MshA from Corynebacterium glutamicum was determined both in the absence of substrates and in a complex with UDP and 1-l-myo-inositol-1-phosphate. MshA belongs to the GT-B structural family whose members have a two-domain structure with both domains exhibiting a Rossman-type fold. Binding of the donor sugar to the C-terminal domain produces a 97 rotational reorientation of the N-terminal domain relative to the C-terminal domain, clamping down on UDP and generating the binding site for 1-l-myo-inositol-1-phosphate. The structure highlights the residues important in binding of UDP-N-acetylglucosamine and 1-l-myo-inositol-1-phosphate. Molecular models of the ternary complex suggest a mechanism in which the {beta}-phosphate of the substrate, UDP-N-acetylglucosamine, promotes the nucleophilic attack of the 3-hydroxyl group of 1-l-myo-inositol-1-phosphate while at the same time promoting the cleavage of the sugar nucleotide bond.

  11. A Mononucleotide Markers Panel to Identify hMLH1/hMSH2 Germline Mutations

    Directory of Open Access Journals (Sweden)

    M. Pedroni


    Full Text Available Hereditary NonPolyposis Colorectal Cancer (Lynch syndrome is an autosomal dominant disease caused by germline mutations in a class of genes deputed to maintain genomic integrity during cell replication, mutations result in a generalized genomic instability, particularly evident at microsatellite loci (Microsatellite Instability, MSI. MSI is present in 85–90% of colorectal cancers that occur in Lynch Syndrome. To standardize the molecular diagnosis of MSI, a panel of 5 microsatellite markers was proposed (known as the “Bethesda panel”. Aim of our study is to evaluate if MSI testing with two mononucleotide markers, such as BAT25 and BAT26, was sufficient to identify patients with hMLH1/hMSH2 germline mutations. We tested 105 tumours for MSI using both the Bethesda markers and the two mononucleotide markers BAT25 and BAT26. Moreover, immunohistochemical evaluation of MLH1 and MSH2 proteins was executed on the tumours with at least one unstable microsatellite, whereas germline hMLH1/hMSH2 mutations were searched for all cases showing two or more unstable microsatellites.

  12. Supernova remnants, pulsar wind nebulae and their interaction

    NARCIS (Netherlands)

    Swaluw, E. van der


    A supernova explosion marks the end of the evolution of a massive star. What remains of the exploded star is a high density neutron star or a black hole. The material which has been ejected by the supernova explosion will manifest itself as a supernova remnant: a hot bubble of gas expanding in the

  13. Pulsar Wind Nebulae, Space Velocities and Supernova Remnant Associations (United States)


    I am pleased to be able to report significant progress in my research relevant to my LTSA grant. This progress I believe is demonstrated by a long list of publications in 2002, as detailed below. I summarize the research results my collaborators and I obtained in 2002. First, my group announced the major discovery of soft-gamma-repeater-like X-ray bursts from the anomalous X-ray pulsars lE-1048.1$-$5937 and lE-2259+586, using the Rossi X-ray Timing Explorer. This result provides an elegant and long-sought-after confirmation that this class of objects and the soft gamma repeaters share a common nature, namely that they are magnetars. Magnetars are a novel manifestation of young neutron stars, quite different from conventional Crab-like radio pulsars. This discovery was made as part of our regular monitoring program, among the goals of which was to detect such outbursts.

  14. An IFU-view of Planetary Nebulae: Exploring NGC 6720 (Ring Nebula) with KCWI (United States)

    Hoadley, Keri; Matuszewski, Matt; Hamden, Erika; Martin, Christopher; Neill, Don; Kyne, Gillian


    Studying the interaction between the ejected stellar material and interstellar clouds is important for understanding how stellar deaths influences the pollution of matter that will later form other stars. Planetary nebulae provide ideal laboratories to study such interactions. I will present on a case study of one close-by planetary nebula, the Ring Nebula (M 57, NGC 6720), to infer the abundances, temperatures, structures, and dynamics of important atomic and ionic species in two distinct regions of the nebula using a newly-commissioned integral field spectrograph (IFS) on Keck: the Keck Cosmic Web Imager (KCWI). The advantage of an IFS over traditional filter-imaging techniques is the ability to simultaneously observe the spectrum of any given pixel in the imaging area, which provides crucial information about the dynamics of the observed region. This technique is powerful for diffuse or extended astrophysical objects, and I will demonstrate the different imaging and spectral modes of KCWI used to observe the Ring Nebula.KCWI observations of the Ring Nebula focused mainly on the innermost region of the nebula, with a little coverage of the Inner Ring. We also observed the length of the Ring in one set of observations, for which we will estimate the elemental abundances, temperatures, and dynamics of the region. KCWI observations also capture an inner arc and blob that have distinctly difference characteristics than the Ring itself and may be a direct observation of either the planetary nebula ramming into an interstellar cloud projected onto the sightline or a dense interstellar cloud being illuminated by the stellar continuum from the hot central white dwarf.

  15. Optical and X-ray luminosities of expanding nebulae around ultraluminous X-ray sources (United States)

    Siwek, Magdalena; Sądowski, Aleksander; Narayan, Ramesh; Roberts, Timothy P.; Soria, Roberto


    We have performed a set of simulations of expanding, spherically symmetric nebulae inflated by winds from accreting black holes in ultraluminous X-ray sources (ULXs). We implemented a realistic cooling function to account for free-free and bound-free cooling. For all model parameters we considered, the forward shock in the interstellar medium becomes radiative at a radius ˜100 pc. The emission is primarily in optical and UV, and the radiative luminosity is about 50 per cent of the total kinetic luminosity of the wind. In contrast, the reverse shock in the wind is adiabatic so long as the terminal outflow velocity of the wind vw ≳ 0.003c. The shocked wind in these models radiates in X-rays, but with a luminosity of only ˜1035 erg s-1. For wind velocities vw ≲ 0.001c, the shocked wind becomes radiative, but it is no longer hot enough to produce X-rays. Instead it emits in optical and UV, and the radiative luminosity is comparable to 100 per cent of the wind kinetic luminosity. We suggest that measuring the optical luminosities and putting limits on the X-ray and radio emission from shock-ionized ULX bubbles may help in estimating the mass outflow rate of the central accretion disc and the velocity of the outflow.

  16. A nebula of gases from Io surrounding Jupiter. (United States)

    Krimigis, Stamatios M; Mitchell, Donald G; Hamilton, Douglas C; Dandouras, Jannis; Armstrong, Thomas P; Bolton, Scott J; Cheng, Andrew F; Gloeckler, George; Hsieh, K C; Keath, Edwin P; Krupp, Norbert; Lagg, Andreas; Lanzerotti, Louis J; Livi, Stefano; Mauk, Barry H; McEntire, Richard W; Roelof, Edmond C; Wilken, Berend; Williams, Donald J


    Several planetary missions have reported the presence of substantial numbers of energetic ions and electrons surrounding Jupiter; relativistic electrons are observable up to several astronomical units (au) from the planet. A population of energetic (>30[?]keV) neutral particles also has been reported, but the instrumentation was not able to determine the mass or charge state of the particles, which were subsequently labelled energetic neutral atoms. Although images showing the presence of the trace element sodium were obtained, the source and identity of the neutral atoms---and their overall significance relative to the loss of charged particles from Jupiter's magnetosphere---were unknown. Here we report the discovery by the Cassini spacecraft of a fast (>103[?]km[?]s-1) and hot magnetospheric neutral wind extending more than 0.5[?]au from Jupiter, and the presence of energetic neutral atoms (both hot and cold) that have been accelerated by the electric field in the solar wind. We suggest that these atoms originate in volcanic gases from Io, undergo significant evolution through various electromagnetic interactions, escape Jupiter's magnetosphere and then populate the environment around the planet. Thus a 'nebula' is created that extends outwards over hundreds of jovian radii.

  17. Abundances in planetary nebulae : NGC 6826

    NARCIS (Netherlands)

    Surendiranath, R.; Pottasch, S. R.

    Aims. We determine the chemical abundances and other parameters of the nebula NGC 6826 and its central star. Methods. We present new ISO spectra and combine them with archival IUE and optical spectra from the literature to get a complete, extinction-corrected, spectrum. The chemical composition of

  18. Abundances of planetary nebula NGC2392

    NARCIS (Netherlands)

    Pottasch, S. R.; Bernard-Salas, J.; Roellig, T. L.

    The spectra of the planetary nebula NGC2392 is reanalysed using spectral measurements made in the mid-infrared with the Spitzer Space Telescope. The aim is to determine the chemical composition of this object. We also make use of IUE and ground based spectra. Abundances determined from the

  19. Abundances in planetary nebulae : NGC 2792

    NARCIS (Netherlands)

    Pottasch, S. R.; Surendiranath, R.; Bernard-Salas, J.; Roellig, T. L.

    The mid-infrared spectrum of the rather circular planetary nebula NGC2792 taken with the Spitzer Space Telescope is presented. This spectrum is combined with the ultraviolet IUE spectrum and with the spectrum in the visual wavelength region to obtain a complete, extinction corrected, spectrum. The

  20. Argon and neon in Galactic nebulae (United States)

    Simpson, Janet P.; Bregman, Jesse D.; Dinerstein, H. L.; Lester, Dan F.; Rank, David M.; Witteborn, F. C.; Wooden, D. H.


    KAO observations of the 6.98 micron line of (Ar II), and KAO and ground-based observations of the 8.99 micron line of (Ar III) and the 12.8 micron line of (Ne II) are presented for a number of Galactic H II regions and planetary nebulae.

  1. Challenges in the Identification of MSH6-Associated Colorectal Cancer: Rectal Location, Less Typical Histology, and a Subset With Retained Mismatch Repair Function

    DEFF Research Database (Denmark)

    Klarskov, Louise Laurberg; Holck, Susanne; Bernstein, Inge Thomsen


    Identification of Lynch syndrome tumors is challenging. This relates particularly to MSH6-associated cases, which show reduced penetrance of colorectal cancer and a higher age at diagnosis. We recorded the clinical and morphologic features of 52 MSH6-associated colorectal cancers in comparison wi...... instability and loss of immunohistochemical MSH6 expression. The remaining 8% showed a mismatch repair-stable phenotype, which suggests that analysis of additional tumors might be considered in families suspected of Lynch syndrome....

  2. Comets Kick up Dust in Helix Nebula (United States)


    This infrared image from NASA's Spitzer Space Telescope shows the Helix nebula, a cosmic starlet often photographed by amateur astronomers for its vivid colors and eerie resemblance to a giant eye. The nebula, located about 700 light-years away in the constellation Aquarius, belongs to a class of objects called planetary nebulae. Discovered in the 18th century, these colorful beauties were named for their resemblance to gas-giant planets like Jupiter. Planetary nebulae are the remains of stars that once looked a lot like our sun. When sun-like stars die, they puff out their outer gaseous layers. These layers are heated by the hot core of the dead star, called a white dwarf, and shine with infrared and visible colors. Our own sun will blossom into a planetary nebula when it dies in about five billion years. In Spitzer's infrared view of the Helix nebula, the eye looks more like that of a green monster's. Infrared light from the outer gaseous layers is represented in blues and greens. The white dwarf is visible as a tiny white dot in the center of the picture. The red color in the middle of the eye denotes the final layers of gas blown out when the star died. The brighter red circle in the very center is the glow of a dusty disk circling the white dwarf (the disk itself is too small to be resolved). This dust, discovered by Spitzer's infrared heat-seeking vision, was most likely kicked up by comets that survived the death of their star. Before the star died, its comets and possibly planets would have orbited the star in an orderly fashion. But when the star blew off its outer layers, the icy bodies and outer planets would have been tossed about and into each other, resulting in an ongoing cosmic dust storm. Any inner planets in the system would have burned up or been swallowed as their dying star expanded. So far, the Helix nebula is one of only a few dead-star systems in which evidence for comet survivors has been found. This image is made up of data from Spitzer


    NARCIS (Netherlands)


    We have surveyed two PG 1159 class stars for the presence of ancient planetary nebulae by direct Her imaging. While we easily found an 11' diameter nebula around PG 1520+525, no nebula was detected around PG 1424+535. This nebula is the tenth member of the class of planetary nebulae surrounding PG

  4. The Orion Nebula: The Jewel in the Sword (United States)


    /01 ESO PR Photo 03c/01 [Preview - JPEG: 400 x 452 pix - 57k] [Normal - JPEG: 800 x 904 pix - 488k] [Hires - JPEG: 2300 x 2600 pix - 3.3M] Caption : PR Photo 03b/01 and PR Photo 03c/01 show smaller, particularly interesting areas of PR Photo 03a/01 . Photo 03b/01 shows the traces of a massive outflow of gas from a very young object embedded in the dense molecular cloud behind the Orion Nebula. Shards of gas from the explosion create shocks and leave bow-waves as they move at speeds of up to 200 km/sec from the source. Photo 03c/01 shows the delicate tracery created at the so-called Bright Bar , as the intense UV-light and strong winds from the hot Trapezium stars eat their way into the surrounding molecular cloud. Also visible are a number of very young red objects partly hidden in the cloud, waiting to be revealed as new members of the Trapezium Cluster . Technical information about these photos is available below. Indeed, at visible wavelengths, the dense cluster of stars at the centre is drowned out by the light from the nebula and obscured by remnants of the dust in the gas from which they were formed. However, at longer wavelengths, these obscuring effects are reduced, and the cluster is revealed. In the past couple of years, several of the world's premier ground- and space-based telescopes have made new detailed infrared studies of the Orion Nebula and the Trapezium Cluster , but the VLT image shown here is the "deepest" wide-field image obtained so far. The large collecting area of the VLT and the excellent seeing of the Paranal site combined to yield this beautiful image, packed full of striking details. Powerful explosions and winds from the most massive stars in the region are evident, as well as the contours of gas sculpted by these stars, and more finely focused jets of gas flowing from the smaller stars. Sharper images from the VLT ESO PR Photo 03d/01 ESO PR Photo 03d/01 [Preview - JPEG: 400 x 490 pix - 28k] [Normal - JPEG: 800 x 980 pix - 192k] [Hi

  5. Long Term Osmotic Mini Pump Treatment with Alpha-MSH Improves Myocardial Function in Zucker Diabetic Fatty Rats

    Directory of Open Access Journals (Sweden)

    Miklos Szokol


    Full Text Available The present investigation evaluates the cardiovascular effects of the anorexigenic mediator alpha-melanocyte stimulating hormone (MSH, in a rat model of type 2 diabetes. Osmotic mini pumps delivering MSH or vehicle, for 6 weeks, were surgically implanted in Zucker Diabetic Fatty (ZDF rats. Serum parameters, blood pressure, and weight gain were monitored along with oral glucose tolerance (OGTT. Echocardiography was conducted and, following sacrifice, the effects of treatment on ischemia/reperfusion cardiac injury were assessed using the isolated working heart method. Nicotinamide adenine dinucleotide phosphate (NADPH oxidase activity was measured to evaluate levels of oxidative stress, and force measurements were performed on isolated cardiomyocytes to determine calcium sensitivity, active tension and myofilament co-operation. Vascular status was also evaluated on isolated arterioles using a contractile force measurement setup. The echocardiographic parameters ejection fraction (EF, fractional shortening (FS, isovolumetric relaxation time (IVRT, mitral annular plane systolic excursion (MAPSE, and Tei-index were significantly better in the MSH-treated group compared to ZDF controls. Isolated working heart aortic and coronary flow was increased in treated rats, and higher Hill coefficient indicated better myofilament co-operation in the MSH-treated group. We conclude that MSH improves global heart functions in ZDF rats, but these effects are not related to the vascular status.

  6. Low expression of MSH2 DNA repair protein is associated with poor prognosis in head and neck squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Camila Santos Pereira


    Full Text Available OBJECTIVE: This study aimed to investigate the expression of the MSH2 DNA repair protein in head and neck squamous cell carcinoma (HNSCC in order to analyze its association with clinicopathologic factors and overall survival of patients. MATERIAL AND METHODS: Clinical data and primary lesions of HNSSC were collected from 55 patients who underwent surgical resection with postoperative radiotherapy in Montes Claros, state of Minas Gerais, Brazil, between 2000 and 2008. Immunohistochemical reactions were performed to analyze MSH2 protein expression. RESULTS: Bivariate analysis showed no significant correlation or association between MSH2 expression and clinicopathologic parameters by Mann-Whitney and Kruskal-Wallis tests. Patients with locoregional metastatic disease (OR=4.949, p<0.001 and lower MSH2 immunohistochemical expressions (OR=2.943, p=0.032 presented poorer survival for HNSCC by Cox regression models. CONCLUSIONS: Our data demonstrated that lower MSH2 expression might contribute to a higher clinic aggressiveness of HNSCC by promoting an unfavorable outcome.

  7. 'Peony Nebula' Star Settles for Silver Medal (United States)


    [figure removed for brevity, see original site] [figure removed for brevity, see original site] Poster Version Movie If our galaxy, the Milky Way, were to host its own version of the Olympics, the title for the brightest known star would go to a massive star called Eta Carina. However, a new runner-up now the second-brightest star in our galaxy has been discovered in the galaxy's dusty and frenzied interior. This image from NASA's Spitzer Space Telescope shows the new silver medalist, circled in the inset above, in the central region of our Milky Way. Dubbed the 'Peony nebula' star, this blazing ball of gas shines with the equivalent light of 3.2 million suns. The reigning champ, Eta Carina, produces the equivalent of 4.7 million suns worth of light though astronomers say these estimates are uncertain, and it's possible that the Peony nebula star could be even brighter than Eta Carina. If the Peony star is so bright, why doesn't it stand out more in this view? The answer is dust. This star is located in a very dusty region jam packed with stars. In fact, there could be other super bright stars still hidden deep in the stellar crowd. Spitzer's infrared eyes allowed it to pierce the dust and assess the Peony nebula star's true brightness. Likewise, infrared data from the European Southern Observatory's New Technology Telescope in Chile were integral in calculating the Peony nebula star's luminosity. The Peony nebula, which surrounds the Peony nebular star, is the reddish cloud of dust in and around the white circle. The movie begins by showing a stretch of the dusty and frenzied central region of our Milky Way galaxy. It then zooms in to reveal the 'Peony nebula' star the new second-brightest star in the Milky Way, discovered in part by NASA's Spitzer Space Telescope. This is a three-color composite showing infrared observations from two Spitzer instruments. Blue represents 3.6-micron light and green shows light of 8 microns, both captured by Spitzer's infrared array

  8. Fermi LAT Observations of the Crab Nebula During the Exceptional April 2011 Outburst (United States)

    Hays, Elizabeth


    The Crab Nebula, formerly thought to be steady in gamma rays, shows unexpected and occasionally dramatic variability in high-energy gamma rays. The Large Area Telescope (LAT) on Fermi recorded several strong outbursts, including dedicated pointed observations of the brightest yet seen, a spectacular flare in April 2011. These observations provide a particularly detailed look at the temporal and spectral characteristics of the nebula during the flare. The LAT data show an additional component in the spectral energy distribution that peaks at a maximum of $375\\pm26\\mathrm{MeV}$. In the probable scenario that this component is synchrotron emission, the electrons are accelerated to extreme energies that are difficult to reconcile with the very rapid change in flux and the expectation for acceleration processes and conditions occurring within the pulsar wind nebula. The physical location and mechanism driving the flares remains undetermined despite observations across the spectrum made by a variety of instruments including the Hubble Space Telescope, the Chandra X-ray Observatory, and the Very Large Array. I will present timing and spectral studies of the high-energy gamma-ray data, discuss implications for the origin of the flares, and highlight preparations for the next major flare.

  9. IDH1 mutated low grade astrocytoma occurring in MSH2 mutated Lynch syndrome family

    Directory of Open Access Journals (Sweden)

    Alaa Alkhotani


    Full Text Available Lynch syndrome (LS is an autosomal dominant tumour predisposition syndrome caused by a germline mutation in one of the DNA mismatch repair (MMR genes.Patients with these mutations have an increased risk of brain tumours, the vast majority of which are glioblastomas and medulloblastomas, and their occurrence has been termed Turcot Syndrome. The case presented herein of a member of a Lynch syndrome family with an MSH2 mutation expands the spectrum of brain tumours occurring in Lynch syndrome to include low grade astrocytomas, and is the first reported case of an IDH1 (R132H mutated brain tumour occurring in a Lynch syndrome family.

  10. Assessment of MC1R and α-MSH gene sequences in Iranian vitiligo patients

    Directory of Open Access Journals (Sweden)

    Eskandani M


    Full Text Available Background: Vitiligo is an acquired pigmentary disorder of the skin that is caused by unknown factors and is characterized by white and depigmented patches that enlarge and become more numerous with time. Genetic factors, oxidative stress, autoimmunity, and neurochemical agents, such as catecholamines might also contribute to vitiligo. Cutaneous pigmentation is determined by the amounts of eumelanin and pheomelanin synthesized by the epidermal melanocytes and interference of melanocortin-1 receptor (MC1R, a G-protein coupled receptor, its normal agonist, alpha-melanocyte stimulating hormone (α-MSH, and key enzymes, such as tyrosinase, to protect against sun-induced DNA damage. The MC1R, a 7 pass trans-membrane G-protein coupled receptor, is a key control point in melanogenesis. Loss-of-function mutations at the MC1R are associated with a switch from eumelanin to pheomelanin production, resulting in a red or yellow coat color. Aim: In this research, we aim to examine the genetic variety of MC1R and α-MSH gene in 20 Iranian vitiligo patients and 20 healthy controls. Materials and Methods: Analysis of the MC1R coding gene was performed with direct sequencing. Results: We found the following 9 MC1R coding region variants: Arg163Gl (G488A, Arg227Leu (G680A, Val 97Phe (G289T, Asp184Asn (G550A, Arg227Lys (G680A, Arg142His (G425A, Val60Leu (G178T, Val247Met (C739A, and Val174Ile (G520A. We also found 2 frameshift changes: one of them was the Insertion of C (frameshift in Pro136, stop at Trp148 and the other, Insertion of G (frameshift in Pro256, stop at Trp 333. Of all the changes, the most common was Val60Leu at 5% in patients vs 20% in controls, Val247Met at 15% in patients vs 0% in controls and Val174Ile at 15% in controls and 0% in patients. The other variants showed a frequency <5% in both patients and controls. Also in this study, we have examined the frequency of single nucleotide polymorphisms within the α-MSH genes with direct sequencing in

  11. Aberrant splicing in MLH1 and MSH2 due to exonic and intronic variants. (United States)

    Pagenstecher, Constanze; Wehner, Maria; Friedl, Waltraut; Rahner, Nils; Aretz, Stefan; Friedrichs, Nicolaus; Sengteller, Marlies; Henn, Wolfram; Buettner, Reinhard; Propping, Peter; Mangold, Elisabeth


    Single base substitutions in DNA mismatch repair genes which are predicted to lead either to missense or silent mutations, or to intronic variants outside the highly conserved splicing region are often found in hereditary nonpolyposis colorectal cancer (HNPCC) families. In order to use the variants for predictive testing in persons at risk, their pathogenicity has to be evaluated. There is growing evidence that some substitutions have a detrimental influence on splicing. We examined 19 unclassified variants (UVs) detected in MSH2 or MLH1 genes in patients suspected of HNPCC for expression at RNA level. We demonstrate that 10 of the 19 UVs analyzed affect splicing. For example, the substitution MLH1,c.2103G > C in the last position of exon 18 does not result in a missense mutation as theoretically predicted (p.Gln701His), but leads to a complete loss of exon 18. The substitution MLH1,c.1038G > C (predicted effect p.Gln346His) leads to complete inactivation of the mutant allele by skipping of exons 10 and 11, and by activation of a cryptic intronic splice site. Similarly, the intronic variant MLH1,c.306+2dupT results in loss of exon 3 and a frameshift mutation due to a new splice donor site 5 bp upstream. Furthermore, we confirmed complete exon skipping for the mutations MLH1,c.1731G > A and MLH1,c.677G > A. Partial exon skipping was demonstrated for the mutations MSH2,c.1275A > G, MLH1,c.588+5G > A, MLH1,c.790+4A > G and MLH1,c.1984A > C. In contrast, five missense mutations (MSH2,c.4G > A, MSH2,c.2123T > A, MLH1,c.464T > G, MLH1,c.875T > C and MLH1,c.2210A > T) were found in similar proportions in the mRNA as in the genomic DNA. We conclude that the mRNA examination should precede functional tests at protein level.

  12. Evaluation of the immunogenicity of the synthetic α-melanocyte-stimulating hormone (α-MSH) analogue afamelanotide ([Nle4-D-Phe7]-α-MSH, Scenesse®) in erythropoietic protoporphyria patients by ELISA detecting both anti-afamelanotide and anti-α-MSH antibodies. (United States)

    Lengweiler, Stephan; Kreim, Saskia; Barman-Aksözen, Jasmin; Maurer, Marcus; Minder, Elisabeth I


    Afamelanotide is an α-melanocyte-stimulating hormone (α-MSH) agonist with proven efficacy in photodermatoses such as erythropoietic protoporphyria (EPP). This peptide drug, repeatedly administered over prolonged time, may induce anti-drug antibodies (ADA). Here, we describe a new ELISA method developed to monitor the occurrence of ADA against afamelanotide as well as against α-MSH. Covalent binding instead of absorption of antigen onto the microtitre wells prevented antigen leakage and enabled extensive washings followed by lower background. The cut-off between antibody-negative and -positive sera was determined. Inhibition of the antigen-antibody reaction by excess soluble antigen tested for specificity. The sensitivity of the ELISA was 608 and 1,390 ng/ml of specific ADA against afamelanotide and α-MSH, respectively. This ELISA method enabled us to investigate the occurrence of ADA during long-term administration of afamelanotide. No immunoreactivity was found in 23 of the 26 EPP patients exposed to the drug for up to 6 years. Pre-existing immunoreactivity against afamelanotide as well as α-MSH was found in 3 patients, whose titres did not change during afamelanotide administration. The new ELISA is suitable to determine ADA against afamelanotide and α-MSH. Afamelanotide did not elicit ADA during long-term administration in patients with EPP. © 2014 S. Karger AG, Basel.

  13. Chemical Abundances of Compact Planetary Nebulae (United States)

    Lee, Ting-Hui; Shaw, Richard A.; Stanghellini, letizia; Riley, Ben


    We present preliminary results from an optical spectroscopic survey of compact planetary nebulae (PNe) in the Galactic disk. This is an ongoing optical+infrared spectral survey of 150 compact PNe to build a deep sample of PN chemical abundances. We obtained optical spectra of PNe with the Southern Astrophysical Research (SOAR) Telescope and Goodman High-Throughput Spectrograph between 2012 and 2015. These data were used to calculate the nebulae diagnostics such as electron temperature and density for each PN, and to derive the elemental abundances of He, N, O Ne, S and Ar. These abundances are vital to understanding the nature of the PNe, and their low- to intermediate-mass progenitor stars.

  14. FACT. Energy spectrum of the Crab Nebula

    Energy Technology Data Exchange (ETDEWEB)

    Temme, Fabian; Einecke, Sabrina; Buss, Jens [TU Dortmund, Experimental Physics 5, Otto-Hahn-Str.4, 44221 Dortmund (Germany); Collaboration: FACT-Collaboration


    The First G-APD Cherenkov Telescope is the first Imaging Air Cherenkov Telescope which uses silicon photon detectors (G-APDs aka SiPM) as photo sensors. With more than four years of operation, FACT proved an application of SiPMs is suitable for the field of ground-based gamma-ray astronomy. Due to the stable flux at TeV energies, the Crab Nebula is handled as a ''standard candle'' in Cherenkov astronomy. The analysis of its energy spectrum and comparison with other experiments, allows to evaluate the performance of FACT. A modern analysis chain, based on data stream handling and multivariate analysis methods was developed in close cooperation with the department of computer science at the TU Dortmund. In this talk, this analysis chain and its application are presented. Further to this, results, including the energy spectrum of the Crab Nebula, measured with FACT, are shown.

  15. DNA mismatch repair protein MSH2 dictates cellular survival in response to low dose radiation in endometrial carcinoma cells.

    LENUS (Irish Health Repository)

    Martin, Lynn M


    DNA repair and G2-phase cell cycle checkpoint responses are involved in the manifestation of hyper-radiosensitivity (HRS). The low-dose radioresponse of MSH2 isogenic endometrial carcinoma cell lines was examined. Defects in cell cycle checkpoint activation and the DNA damage response in irradiated cells (0.2 Gy) were evaluated. HRS was expressed solely in MSH2+ cells and was associated with efficient activation of the early G2-phase cell cycle checkpoint. Maintenance of the arrest was associated with persistent MRE11, γH2AX, RAD51 foci at 2 h after irradiation. Persistent MRE11 and RAD51 foci were also evident 24 h after 0.2 Gy. MSH2 significantly enhances cell radiosensitivity to low dose IR.

  16. Multi-Zone Modeling of the Pulsar Win Nebula HESS J1825-137

    Energy Technology Data Exchange (ETDEWEB)

    Van Etten, Adam; Romani, Roger W.; /Stanford U., Phys. Dept.


    The pulsar wind nebula associated with PSR J1826-1334, HESS J1825-137, is a bright very high energy source with an angular extent of {approx} 1{sup o} and spatially-resolved spectroscopic TeV measurements. The gamma-ray spectral index is observed to soften with increasing distance from the pulsar, likely the result of cooling losses as electrons traverse the nebula. We describe analysis of X-ray data of the extended nebula, as well as 3-D time-dependent spectral energy distribution modeling, with emphasis on the spatial variations within HESS J1825-137. The multi-wavelength data places significant constraints on electron injection, transport, and cooling within the nebula. The large size and high nebular energy budget imply a relatively rapid initial pulsar spin period of 13 {+-} 7 ms and an age of 40 {+-} 9 kyr. The relative fluxes of each VHE zone can be explained by advective particle transport with a radially decreasing velocity profile with v(r) {proportional_to} r{sup -0.5}. The evolution of the cooling break requires an evolving magnetic field which also decreases radially from the pulsar, B(r, t) {proportional_to} r{sup -0.7} E(t){sup 1/2}. Detection of 10 TeV flux {approx} 80 pc from the pulsar requires rapid diffusion of high energy particles with {tau}{sub esc} {approx} 90 (R/10 pc){sup 2}(E{sub e}/100TeV){sup -1} year, contrary to the common assumption of toroidal magnetic fields with strong magnetic confinement. The model predicts a rather uniform Fermi LAT surface brightness out to {approx} 1{sup o} from the pulsar, in good agreement with the recently discovered LAT source centered 0.5{sup o} southwest of PSR J1826-1334 with extension 0.6 {+-} 0.1{sup o}.

  17. The radio and infrared counterparts of the ring nebula around HD211564 (United States)

    Cappa, C. E.; Vasquez, J.; Pineault, S.; Cichowolski, S.


    We report the detection of the radio and infrared (IR) counterparts of the ring nebula around the WN3(h) star HD211564 (WR152), located to the south-west of the HII region Sh2132. Using radio continuum data from the Canadian Galactic Plane Survey, we identified the radio counterparts of the two concentric rings, of about 9 and 16 arcmin in radius, related to the star. After applying a filling factor f = 0.05-0.12, electron densities and ionized masses are in the range 10-16 cm-3 and 450-700Msolar, respectively. The analysis of the HI gas emission distribution allowed the identification of 5900Msolar of neutral atomic gas with velocities between -52 and -43kms-1 probably linked to the nebula. The region of the nebula is almost free of molecular gas. Only four small clumps were detected, with a total molecular mass of 790Msolar. About 310 Msolar are related to a small IR shell-like source linked to the inner ring, which is also detected in the MSX band A. An IRAS young stellar object candidate is detected in coincidence with the shell-like IR source. We suggest that the optical nebula and its neutral counterparts originated from the stellar winds from the Wolf-Rayet star and its massive progenitor, and are evolving in the envelope of a slowly expanding shell centred at of about 31 pc in radius. The bubble's energy conversion efficiency is in agreement with recent numerical analysis and with observational results.

  18. The Remarkable Synchrotron Nebula Associated with PSR J1015-5719 (United States)

    Ng, Chi Yung; Bandiera, Rino; Hunstead, Richard; Johnston, Simon


    We report the discovery of a synchrotron nebula G283.1-0.59 associated with the young and energetic pulsar J1015-5719. Radio observations using the Molonglo Observatory Synthesis Telescope (MOST) and the Australia Telescope Compact Array (ATCA) at 36, 16, 6, and 3 cm reveal a complex morphology for the source. The pulsar is embedded in the "head" of the nebula with fan-shaped diffuse emission. This is connected to a circular bubble structure of 20" radius and followed by a collimated tail extending over 1'. Polarization measurements show a highly ordered magnetic field in the nebula. The intrinsic B-field wraps around the edge of the head and shows an azimuthal configuration near the pulsar, then switches direction quasi-periodically near the bubble and in the tail. Together with the flat radio spectrum observed, we suggest that this system is most plausibly a pulsar wind nebula (PWN), with the head as a bow shock that has a low Mach number and the bubble as a shell expanding in a dense environment, possibly due to flow instabilities. In addition, the bubble could act as a magnetic bottle trapping the relativistic particles. A comparison with other bow-shock PWNe with higher Mach numbers shows similar structure and B-field geometry, implying that pulsar velocity may not be the most critical factor in determining the properties of these systems.ATCA is part of the Australia Telescope National Facility which is funded by the Commonwealth of Australia for operation as a National Facility managed by CSIRO. MOST is operated by The University of Sydney with support from the Australian Research Council and the Science Foundation for Physics within the University of Sydney. This work is supported by an ECS grant under HKU 709713P.

  19. Rossby Waves in the Protoplanetary Nebula (United States)

    Sheehan, Daniel P.


    Fluid waves and instabilities are considered critical to the evolution of protoplanetary nebulae, particularly for their roles in mass, angular momentum, and energy transport. A number have been identified, however, notably absent, is an influential wave commonly found in planetary atmospheres and oceans: the planetary Rossby wave (PRW). Since, in the Earth's atmosphere, the PRW is of primary importance in shaping large-scale meteorological phenomena, it is reasonable to consider whether it might have similar importance in the protoplanetary nebula. The thrust of the research project this summer (1998) was to determine whether a nebular analog to the PRW is viable, a so-called nebular Rossby wave (NRW), and if so, to explore possible ramifications of this wave to the evolution of the nebula. This work was carried out primarily by S. Davis, J. Cuzzi and me, with significant discussions with P. Cassen. We believe we have established a good case for the NRW and as a result believe we have opened up a new and possibly interesting line of research in regard to the nebular development, in particular with regard to zonal jet formation, a potent accretion mechanism, and possible ties to vortex formation. The standard model of the protoplanetary nebula consists of a large disk of gas with about 1% entrained dust gravitationally bound to a large central mass, m(sub c) i.e., the protostar. The planet-forming region of the disk extends to roughly 100 A.U. in radius. Disk thickness, H, is believed to be on the order of 10-100 times less than disk radius. Disk lifetime is on the order of a million years.

  20. Nebula observations. Catalogues and archive of photoplates (United States)

    Shlyapnikov, A. A.; Smirnova, M. A.; Elizarova, N. V.


    A process of data systematization based on "Academician G.A. Shajn's Plan" for studying the Galaxy structure related to nebula observations is considered. The creation of digital versions of catalogues of observations and publications is described, as well as their presentation in HTML, VOTable and AJS formats and basic principles of work in the interactive application of International Virtual Observatory the Aladin Sky Atlas.

  1. Nebula Instruction Set Architecture (ISA) Evaluation. (United States)


    Shaman , P., Lamb, D., "Evaluation of Computer Archi- tectures Via Test Programs," 1977 National Computer Conference Pro- ceedings, Volume 46...times" is a Chinese curse not usually appreciated by westerners, unless they have been software project managers. 11-2.9 .o *0 ,. o...%* ". , "" -" 4...8217 ., ". :" .-’ .". ,.,.. , . , " ,,. .-. . . . -, . ’ .-. • , - . ,. ... . - ,.. ... , , . ", .. ,.- . , .. •. ,. ., .,. ., . -,. WU . " .wr.’.-..\\.. -. . -- w . 7.j" . Nebula and Portability

  2. High scale anisotropies in planetary nebulae

    Energy Technology Data Exchange (ETDEWEB)

    Pascoli, G.


    We present a new classification of Planetary Nebulae (PN) grounded on their characteristic symmetries: bipolarity, ring shape, spiral structure, etc... The different anisotropic models (rotation of nucleus, binary progenitor intranebular magnetic field, nebular rotation, etc...) which have been lately proposed, are analysed and their explanatory power is tested with certain morphological criterious. The comparison with the other classifications (Acker, 1980; Kaler, 1978; Peimbert, 1978) reveals that the morphology has been insufficiently discussed in these latters.

  3. Prevalence of pathological germline mutations of hMLH1 and hMSH2 genes in colorectal cancer.

    Directory of Open Access Journals (Sweden)

    Dandan Li

    Full Text Available The prevalence of pathological germline mutations in colorectal cancer has been widely studied, as germline mutations in the DNA mismatch repair genes hMLH1 and hMSH2 confer a high risk of colorectal cancer. However, because the sample size and population of previous studies are very different from each other, the conclusions still remain controversial. In this paper, Databases such as PubMed were applied to search for related papers. The data were imported into Comprehensive Meta-Analysis V2, which was used to estimate the weighted prevalence of hMLH1 and hMSH2 pathological mutations and compare the differences of prevalence among different family histories, ethnicities and related factors. This study collected and utilized data from 102 papers. In the Amsterdam-criteria positive group, the prevalence of pathological germline mutations of the hMLH1 and hMSH2 genes was 28.55% (95%CI 26.04%-31.19% and 19.41% (95%CI 15.88%-23.51%, respectively, and the prevalence of germline mutations in hMLH1/hMSH2 was 15.44%/10.02%, 20.43%/13.26% and 15.43%/11.70% in Asian, American multiethnic and European/Australian populations, respectively. Substitution mutations accounted for the largest proportion of germline mutations (hMLH1: 52.34%, hMSH2: 43.25%. The total prevalence of mutations of hMLH1 and hMSH2 in Amsterdam-criteria positive, Amsterdam-criteria negative and sporadic colorectal cancers was around 45%, 25% and 15%, respectively, and there were no obvious differences in the prevalence of germline mutations among different ethnicities.

  4. Compound heterozygosity for two MSH6 mutations in a patient with early onset colorectal cancer, vitiligo and systemic lupus erythematosus. (United States)

    Rahner, Nils; Höefler, Gerald; Högenauer, Christoph; Lackner, Caroline; Steinke, Verena; Sengteller, Marlies; Friedl, Waltraut; Aretz, Stefan; Propping, Peter; Mangold, Elisabeth; Walldorf, Constanze


    Lynch syndrome (hereditary non-polyposis colorectal cancer, HNPCC) is an autosomal dominant condition caused by heterozygous germline mutations in the DNA mismatch repair (MMR) genes MLH1, MSH2, MSH6, or PMS2. Rare cases have been reported of an inherited bi-allelic deficiency of MMR genes, associated with multiple café-au-lait spots, early onset CNS tumors, hematological malignancies, and early onset gastrointestinal neoplasia. We report on a patient with vitiligo in segments of the integument who developed systemic lupus erythematosus (SLE) at the age of 16, and four synchronous colorectal cancers at age 17 years. Examination of the colorectal cancer tissue showed high microsatellite instability (MSI-H) and an exclusive loss of expression of the MSH6 protein. Immunohistochemical analysis of normal colon tissue also showed loss of MSH6, pointing to a bi-allelic MSH6 mutation. Sequencing of the MSH6 gene showed the two germline mutations; c.1806_1809delAAAG;p.Glu604LeufsX5 and c.3226C > T;p.Arg1076Cys. We confirmed that the two mutations are on two different alleles by allele-specific PCR. To our knowledge, neither parent is clinically affected. They did not wish to be tested for the mutations identified in their daughter. These data suggest that bi-allelic mutations of one of the MMR genes should be considered in patients who develop early-onset multiple HNPCC-associated tumors and autoimmune disorders, even in absence of either hematological malignancies or brain tumors. 2008 Wiley-Liss, Inc.

  5. Neuropeptide Y and α-MSH circadian levels in two populations with low body weight: anorexia nervosa and constitutional thinness.

    Directory of Open Access Journals (Sweden)

    Bogdan Galusca

    Full Text Available Anorexia nervosa (AN presents an adaptive appetite regulating profile including high levels of ghrelin and 26RFa (orexigenic and low levels of leptin and PYY (anorexigenic. However, this adaptive mechanism is not effective in promoting food intake. The NPY/proopiomelanocortin (POMC system plays a crucial role in the regulation of feeding behavior as NPY is the most potent orexigenic neuropeptide identified so far and as the POMC-derived peptide α-MSH drastically reduces food intake, and this peptidergic system has not been thoroughly studied in AN.The aim of the present study was thus to investigate whether a dysfunction of the NPY/POMC occurs in two populations with low body weight, AN and constitutional thinness (CT.This was a cross-sectional study performed in an endocrinological unit and in an academic laboratory.Three groups of age-matched young women were studied: 23 with AN (AN, 22 CT and 14 normal weight controls.Twelve-point circadian profiles of plasma NPY and α-MSH levels were measured in the three groups of investigated subjects.No significant circadian variation of NPY was detected between the three groups. Plasma α-MSH levels were significantly lower in AN (vs controls all over the day. The CT group, compared to controls, presented lower levels of α-MSH in the morning and the evening, and an important rise during lunchtime.In AN patients, the NPY system is not up-regulated under chronic undernutrition suggesting that this may play a role in the inability of anorectic women to adapt food intake to their energy demand. In contrast, low circadian α-MSH levels integrate the adaptive profile of appetite regulation of this disease. Finally, in CT women, the important α-MSH peak detected during lunchtime could explain why these patients are rapidly food satisfied.

  6. of Planetary Nebulae III. NGC 6781

    Directory of Open Access Journals (Sweden)

    Hugo E. Schwarz


    Full Text Available Continuing our series of papers on the three-dimensional (3D structures and accurate distances to Planetary Nebulae (PNe, we present our study of the planetary nebula NGC6781. For this object we construct a 3D photoionization model and, using the constraints provided by observational data from the literature we determine the detailed 3D structure of the nebula, the physical parameters of the ionizing source and the first precise distance. The procedure consists in simultaneously fitting all the observed emission line morphologies, integrated intensities and the two-dimensional (2D density map from the [SII] (sulfur II line ratios to the parameters generated by the model, and in an iterative way obtain the best fit for the central star parameters and the distance to NGC6781, obtaining values of 950±143 pc (parsec – astronomic distance unit and 385 LΘ (solar luminosity for the distance and luminosity of the central star respectively. Using theoretical evolutionary tracks of intermediate and low mass stars, we derive the mass of the central star of NGC6781 and its progenitor to be 0.60±0.03MΘ (solar mass and 1.5±0.5MΘ respectively.

  7. 3He Abundances in Planetary Nebulae (United States)

    Guzman-Ramirez, Lizette


    Determination of the 3He isotope is important to many fields of astrophysics, including stellar evolution, chemical evolution, and cosmology. The isotope is produced in stars which evolve through the planetary nebula phase. Planetary nebulae are the final evolutionary phase of low- and intermediate-mass stars, where the extensive mass lost by the star on the asymptotic giant branch is ionised by the emerging white dwarf. This ejecta quickly disperses and merges with the surrounding ISM. 3He abundances in planetary nebulae have been derived from the hyperfine transition of the ionised 3He, 3He+, at the radio rest frequency 8.665 GHz. 3He abundances in PNe can help test models of the chemical evolution of the Galaxy. Many hours have been put into trying to detect this line, using telescopes like the Effelsberg 100m dish of the Max Planck Institute for Radio Astronomy, the National Radio Astronomy Observatory (NRAO) 140-foot telescope, the NRAO Very Large Array, the Arecibo antenna, the Green Bank Telescope, and only just recently, the Deep Space Station 63 antenna from the Madrid Deep Space Communications Complex.

  8. Beneficial effect of chronic treatment with Org 2766 and alpha-MSH on impaired reversal learning of rats with bilateral lesions of the parafascicular area

    NARCIS (Netherlands)

    Nyakas, C; Veldhuis, H D; De Wied, D

    The effects of chronic treatment with the ACTH-(4-9) analogue Org 2766, alpha-MSH, and gamma 2-MSH were studied on T-maze reversal learning and on behavior assessed on the basis of open-field and other gross behavioral activities, grasping responses, inspection of various reflexes and electrical

  9. Very low prevalence of germline MSH6 mutations in hereditary non-polyposis colorectal cancer suspected patients with colorectal cancer without microsatellite instability.

    NARCIS (Netherlands)

    Kets, M.; Krieken, J.H.J.M. van; Hebeda, K.M.; Wezenberg, S.J.; Goossens, M.; Brunner, H.G.; Ligtenberg, M.J.L.; Hoogerbrugge-van der Linden, N.


    Hereditary non-polyposis colorectal cancer (HNPCC) is caused by mutations in one of the mismatch repair genes MLH1, MSH2, MSH6, or PMS2 and results in high-level microsatellite instability (MSI-high) in tumours of HNPCC patients. The MSI test is considered reliable for indicating mutations in MLH1

  10. Particle acceleration model for the broad-band baseline spectrum of the Crab nebula (United States)

    Fraschetti, F.; Pohl, M.


    We develop a simple one-zone model of the steady-state Crab nebula spectrum encompassing both the radio/soft X-ray and the GeV/multi-TeV observations. By solving the transport equation for GeV-TeV electrons injected at the wind termination shock as a log-parabola momentum distribution and evolved via energy losses, we determine analytically the resulting differential energy spectrum of photons. We find an impressive agreement with the observed spectrum of synchrotron emission, and the synchrotron self-Compton component reproduces the previously unexplained broad 200-GeV peak that matches the Fermi/Large Area Telescope (LAT) data beyond 1 GeV with the Major Atmospheric Gamma Imaging Cherenkov (MAGIC) data. We determine the parameters of the single log-parabola electron injection distribution, in contrast with multiple broken power-law electron spectra proposed in the literature. The resulting photon differential spectrum provides a natural interpretation of the deviation from power law customarily fitted with empirical multiple broken power laws. Our model can be applied to the radio-to-multi-TeV spectrum of a variety of astrophysical outflows, including pulsar wind nebulae and supernova remnants, as well as to interplanetary shocks.

  11. Abundances in planetary nebulae : Me 2-1

    NARCIS (Netherlands)

    Surendiranath, R; Pottasch, [No Value; Garcia-Lario, P

    ISO and IUE spectra of the round planetary nebula Me 2-1 are combined with Visual spectra taken from the literature to obtain for the first time a complete extinction-corrected spectrum. With this, the physico-chemical characteristics of the nebula and its central star are determined by various

  12. Abundances of the planetary nebula Hu 1-2

    NARCIS (Netherlands)

    Pottasch, [No Value; Hyung, S; Aller, LH; Beintema, DA; Bernard-Salas, J; Feibelman, WA; Klockner, HR

    The ISO and IUE spectra of the "elliptical" nebula Hu 1-2 are presented. These spectra are combined with new, high resolution spectra in the visual wavelength region to obtain a complete, extinction corrected, spectrum. The chemical composition of the nebula is then calculated and compared to

  13. Optical observations of planetary nebula candidates from the northern hemisphere

    NARCIS (Netherlands)

    VandeSteene, GC; Jacoby, GH; Pottasch, [No Value

    We present H alpha+[N II] images of 17 and low resolution spectra of 14 IRAS-selected planetary nebula candidates. The H alpha+[N II] images are presented as finding charts. Contour plots are shown for the resolved planetary nebulae. From these images accurate optical positions and mean optical

  14. An Analysis of Spectra in the Red Rectangle Nebula

    Indian Academy of Sciences (India)


    Jan 27, 2016 ... This paper presents an analysis of a series of spectra in the Red Rectangle nebula. Only the reddest part of the spectra can safely be attributed to light from the nebula, and indicates Rayleigh scattering by the gas, in conformity with the large angles of scattering involved and the proximity of the star. In the ...

  15. The Planetary Nebula Spectrograph : The green light for galaxy kinematics

    NARCIS (Netherlands)

    Douglas, NG; Arnaboldi, M; Freeman, KC; Kuijken, K; Merrifield, MR; Romanowsky, AJ; Taylor, K; Capaccioli, M; Axelrod, T; Gilmozzi, R; Hart, J; Bloxham, G; Jones, D


    Planetary nebulae (PNe) are now well established as probes of galaxy dynamics and as standard candles in distance determinations. Motivated by the need to improve the efficiency of planetary nebulae searches and the speed with which their radial velocities are determined, a dedicated instrument-the

  16. Melanoma prevention by MC1R selective small peptide analogs of alpha MSH | Division of Cancer Prevention (United States)

    DESCRIPTION (provided by applicant): This revised application will test the hypothesis that small peptide analogs of ?-melanocortin (?-MSH) that are selective agonists of the melanocortin 1 receptor (MC1R) will prevent melanoma tumor formation in transgenic mouse melanoma models by enhancing repair of ultraviolet radiation (UV)-induced DNA damage and stimulating melanogenesis. |

  17. Infundibular neurons of the human hypothalamus simultaneously reactive with antisera against endorphins, ACTH, MSH and beta-LPH. (United States)

    Bugnon, C; Bloch, B; Lenys, D; Fellmann, D


    In man, discrete neurons of the infundibular (arcuate) nucleus contain compounds that can be stained with anti-endorphin (alpha and beta), anti-ACTH, anti-MSH (alpha and beta) and anti-beta-LPH immune sera (I.S.). In the fetus, certain neurons stain with anti-beta-endorphin or anti((17--39)ACTH starting from the 11th week of fetal life. At the ultrastructural level, these neurons contain elementary granules that are immunoreactive with anti-beta-endorphin. In the adult, neurons immunoreactive with anti-beta-endorphin are found in the infundibular nucleus. Their axonal fibers terminate around blood vessels in the neurovascular zone and in the pituitary stalk, or establish contacts with non-immunoreactive perikarya of the infundibular nucleus. These neurons can be stained with anti(17--39)ACTH and anti-beta-endorphin I.S. The most reactive are also stained moderately with anti-alpha-MSH, anti-beta-MSH, anti-beta-LPH, anti-alpha-endorphin, or anti(1--24)ACTH I.S. These results indicate that, in man, compound(s) identical with or immunologically related to endorphins, beta-LPH, ACTH and MSH are secreted by certain hypothalamic neurons. These agents probably originate from a common precursor molecula similar to the so-called pro-opiocortin.

  18. Shewanella Oneidensis MR-1 Msh Pilin Proteins are Involved in Extracellular Electron Transfer in Microbial Fuel Cells (United States)


    purchased from ATCC (700550) and the two Msh deletion mutants were gifts from Dr. Daad Saffarini (University of Wisconsin-Milwaukee). In-frame chromosomal...than the actual extracellular structural pilin proteins (nanofilaments). Acknowledgments We would like to thank Daad Saffarini, Rachida Bouhenni and

  19. Spectrum of MLH1 and MSH2 mutations in Chilean families with suspected Lynch syndrome. (United States)

    Alvarez, Karin; Hurtado, Claudia; Hevia, Montserrat A; Wielandt, Ana Maria; de la Fuente, Marjorie; Church, James; Carvallo, Pilar; López-Köstner, Francisco


    Lynch syndrome is the most common inherited syndrome of colorectal cancer, caused principally by germline mutations in MLH1 and MSH2. We report our experience with genetic screening in the diagnosis of Lynch syndrome in Chile, a country previously underserved in the capacity to diagnose hereditary colorectal cancer. Families from our Familial Colorectal Cancer Registry were selected for this study if they fulfilled either Amsterdam I/II or Bethesda criteria for classification of Lynch syndrome. Analysis of colorectal tumors from probands included a microsatellite instability study and immunohistochemical evaluation for MLH1 and MSH2. Screening of germline mutations was performed by single-strand conformation polymorphism analysis and DNA sequencing. A total of 21 families were evaluated, 14 meeting Amsterdam criteria and 7 meeting Bethesda criteria. Tumors in 20 families (95%) showed microsatellite instability (19 high and 1 low) and 9 of these 20 families (45%) harbored a germline mutation (7 of 13 Amsterdam and 2 of 7 Bethesda families). Of the 9 mutations identified, 6 were in MLH1 and 3 in MSH2. Two of the mutations were novel, 3 were previously found in 1 to 2 European populations, and 4 were previously found in various ethnic populations worldwide. Only 2 mutations were previously found in another Latin American population (Colombia). In our probands, colorectal cancer was located mainly (57%) in the right or transverse colon. Pedigree information from 104 family affected members of 21 studied families showed endometrial cancer to be the most frequent primary extracolonic tumor, accounting for 15.1% of total cases, followed by stomach (13.2%) and breast cancer (11.3%). Analysis of mitochondrial DNA haplotypes showed a strong Amerindian genetic component in 15 (71.4%) of the 21 families analyzed. The study of Lynch syndrome in families of different ethnic origins contributes to the definition of genetic and clinical differences among populations. Wide


    Eskandani, M; Hasannia, S; Vandghanooni, S; Pirooznia, N; Golchai, J


    Vitiligo is an acquired pigmentary disorder of the skin that is caused by unknown factors and is characterized by white and depigmented patches that enlarge and become more numerous with time. Genetic factors, oxidative stress, autoimmunity, and neurochemical agents, such as catecholamines might also contribute to vitiligo. Cutaneous pigmentation is determined by the amounts of eumelanin and pheomelanin synthesized by the epidermal melanocytes and interference of melanocortin-1 receptor (MC1R), a G-protein coupled receptor, its normal agonist, alpha-melanocyte stimulating hormone (α-MSH), and key enzymes, such as tyrosinase, to protect against sun-induced DNA damage. The MC1R, a 7 pass trans-membrane G-protein coupled receptor, is a key control point in melanogenesis. Loss-of-function mutations at the MC1R are associated with a switch from eumelanin to pheomelanin production, resulting in a red or yellow coat color. In this research, we aim to examine the genetic variety of MC1R and α-MSH gene in 20 Iranian vitiligo patients and 20 healthy controls. Analysis of the MC1R coding gene was performed with direct sequencing. We found the following 9 MC1R coding region variants: Arg163Gl (G488A), Arg227Leu (G680A), Val 97Phe (G289T), Asp184Asn (G550A), Arg227Lys (G680A), Arg142His (G425A), Val60Leu (G178T), Val247Met (C739A), and Val174Ile (G520A). We also found 2 frameshift changes: one of them was the Insertion of C (frameshift in Pro136, stop at Trp148) and the other, Insertion of G (frameshift in Pro256, stop at Trp 333). Of all the changes, the most common was Val60Leu at 5% in patients vs 20% in controls, Val247Met at 15% in patients vs 0% in controls and Val174Ile at 15% in controls and 0% in patients. The other variants showed a frequency MC1R and α-MSH genes and their effect on the disease in Iranian vitiligo patients.

  1. Emission Lines of Northern Planetary Nebulae (United States)

    Aksaker, Nazim; Yerli, Sinan K.; Kızıloǧlu, Ümit; Atalay, Betül


    In this work, we present results of long slit spectrophotometric emission line flux observations of selected planetary nebulae (PNe). We have measured absolute fluxes and equivalent widths (EW) of all observable emission lines. In addition to these observations, electron temperatures (Te), densities (Ne), and chemical abundances were also calculated. The main purpose of this work is to fill the gaps in emission line flux standards for the northern hemisphere. It is expected that the measured fluxes would be used as standard data set for further photometric and spectrometric measurements of HII regions, supernova remnants etc.

  2. Identification of a deletion in the mismatch repair gene, MSH2, using mouse-human cell hybrids monosomal for chromosome 2. (United States)

    Pyatt, R E; Nakagawa, H; Hampel, H; Sedra, M; Fuchik, M B; Comeras, I; de la Chapelle, A; Prior, T W


    Hereditary non-polyposis colorectal cancer is characterized by mutations in one of the DNA mismatch repair genes, primarily MLH1, MSH2, or MSH6. We report here the identification of a genomic deletion of approximately 11.4 kb encompassing the first two exons of the MSH2 gene in two generations of an Ohio family. By Southern blot analysis, using a cDNA probe spanning the first seven exons of MSH2, an alteration in each of three different enzyme digests (including a unique 13-kb band on HindIII digests) was observed, which suggested the presence of a large alteration in the 5' region of this gene. Mouse-human cell hybrids from a mutation carrier were then generated which contained a single copy each of human chromosome 2 on which the MSH2 gene resides. Southern blots on DNA from the cell hybrids demonstrated the same, unique 13-kb band from one MSH2 allele, as seen in the diploid DNA. DNA from this same monosomal cell hybrid failed to amplify in polymerase chain reactions (PCRs) using primers to exons 1 and 2, demonstrating the deletion of these sequences in one MSH2 allele, and the breakpoints involving Alu repeats were identified by PCR amplification and sequence analysis. Copyright Blackwell Munksgaard, 2003

  3. A C. elegans eIF4E-family member upregulates translation at elevated temperatures of mRNAs encoding MSH-5 and other meiotic crossover proteins (United States)

    Song, Anren; Labella, Sara; Korneeva, Nadejda L.; Keiper, Brett D.; Aamodt, Eric J.; Zetka, Monique; Rhoads, Robert E.


    Caenorhabditis elegans expresses five family members of the translation initiation factor eIF4E whose individual physiological roles are only partially understood. We report a specific role for IFE-2 in a conserved temperature-sensitive meiotic process. ife-2 deletion mutants have severe temperature-sensitive chromosome-segregation defects. Mutant germ cells contain the normal six bivalents at diakinesis at 20°C but 12 univalents at 25°C, indicating a defect in crossover formation. Analysis of chromosome pairing in ife-2 mutants at the permissive and restrictive temperatures reveals no defects. The presence of RAD-51-marked early recombination intermediates and 12 well condensed univalents indicate that IFE-2 is not essential for formation of meiotic double-strand breaks or their repair through homologous recombination but is required for crossover formation. However, RAD-51 foci in ife-2 mutants persist into inappropriately late stages of meiotic prophase at 25°C, similar to mutants defective in MSH-4/HIM-14 and MSH-5, which stabilize a critical intermediate in crossover formation. In wild-type worms, mRNAs for msh-4/him-14 and msh-5 shift from free messenger ribonucleoproteins to polysomes at 25°C but not in ife-2 mutants, suggesting that IFE-2 translationally upregulates synthesis of MSH-4/HIM-14 and MSH-5 at elevated temperatures to stabilize Holliday junctions. This is confirmed by an IFE-2-dependent increase in MSH-5 protein levels. PMID:20530576


    Energy Technology Data Exchange (ETDEWEB)

    Fang, X.; Guerrero, M. A.; Marquez-Lugo, R. A.; Toalá, J. A. [Instituto de Astrofísica de Andalucía (IAA-CSIC), Glorieta de la Astronomía s/n, E-18008 Granada (Spain); Arthur, S. J. [Centro de Radioastronomía y Astrofísica, Universidad Nacional Autónoma de México, Campus Morelia, 58090 Morelia (Mexico); Chu, Y.-H.; Gruendl, R. A. [Department of Astronomy, University of Illinois, 1002 West Green Street, Urbana, IL 61801 (United States); Blair, W. P. [Department of Physics and Astronomy, Johns Hopkins University, Baltimore, MD 21218 (United States); Hamann, W.-R.; Oskinova, L. M.; Todt, H., E-mail: [Institute for Physics and Astronomy, Universität Potsdam, D-14476 Potsdam (Germany)


    We analyze the expansion of hydrogen-poor knots and filaments in the born-again planetary nebulae A30 and A78 based on Hubble Space Telescope (HST) images obtained almost 20 yr apart. The proper motion of these features generally increases with distance to the central star, but the fractional expansion decreases, i.e., the expansion is not homologous. As a result, there is not a unique expansion age, which is estimated to be 610-950 yr for A30 and 600-1140 yr for A78. The knots and filaments have experienced complex dynamical processes: the current fast stellar wind is mass loaded by the material ablated from the inner knots; the ablated material is then swept up until it shocks the inner edges of the outer, hydrogen-rich nebula. The angular expansion of the outer filaments shows a clear dependence on position angle, indicating that the interaction of the stellar wind with the innermost knots channels the wind along preferred directions. The apparent angular expansion of the innermost knots seems to be dominated by the rocket effect of evaporating gas and by the propagation of the ionization front inside them. Radiation-hydrodynamical simulations show that a single ejection of material followed by a rapid onset of the stellar wind and ionizing flux can reproduce the variety of clumps and filaments at different distances from the central star found in A30 and A78.

  5. Urinary Tract Cancer in Lynch Syndrome; Increased Risk in Carriers of MSH2 Mutations

    DEFF Research Database (Denmark)

    Joost, Patrick; Therkildsen, Christina; Dominguez-Valentin, Mev


    OBJECTIVE: To evaluate the risk of urothelial cancer in the upper urinary tract and the bladder, determine the contribution from the different mismatch-repair genes, and define clinical characteristics of urothelial cancer in Lynch syndrome. MATERIALS AND METHODS: The national hereditary...... lifetime risks were determined. RESULTS: In total, 48 cancers of the ureter, 34 cancers of the renal pelvis, and 54 urinary bladder cancers developed at a mean age of 61 (24-89) years. The tumors were typically of high grade, showed loss of mismatch-repair protein expression in 90% of the tumors...... and the urinary bladder are included in the Lynch syndrome tumor spectrum. Urothelial cancers are predominantly linked to MSH2 mutations, which suggest that surveillance should be targeted at individuals with mutations herein....

  6. High rate of CAD gene amplification in human cells deficient in MLH1 or MSH6 (United States)

    Chen, Sihong; Bigner, Sandra H.; Modrich, Paul


    MutS and MutL homologs have been implicated in multiple genetic stabilization pathways. The activities participate in the correction of DNA biosynthetic errors, are involved in cellular responses to certain types of DNA damage, and serve to ensure the fidelity of genetic recombination. We show here that the rate of CAD (carbamyl-P synthetase/aspartate transcarbamylase/dihydroorotase) gene amplification is elevated 50- to 100-fold in human cell lines deficient in MLH1 or MSH6, as compared with mismatch repair-proficient control cells. Fluorescence in situ hybridization indicates that these amplification events are the probable consequence of unequal sister chromatid exchanges involving chromosome 2, as well as translocation events involving other chromosomes. These results implicate MutSα and MutLα in the suppression of gene amplification and suggest that defects in this genetic stabilization function may contribute to the cancer predisposition associated with mismatch repair deficiency. PMID:11717437


    Energy Technology Data Exchange (ETDEWEB)

    Kobulnicky, Henry A.; Chick, William T.; Schurhammer, Danielle P.; Andrews, Julian E.; Munari, Stephan A.; Olivier, Grace M.; Sorber, Rebecca L.; Wernke, Heather N.; Dale, Daniel A. [Dept. of Physics and Astronomy, University of Wyoming, Laramie, WY 82070 (United States); Povich, Matthew S.; Dixon, Don M. [Department of Physics and Astronomy, California State Polytechnic University, 3801 West Temple Avenue, Pomona, CA 91768 (United States)


    We identify 709 arc-shaped mid-infrared nebula in 24 μ m Spitzer Space Telescope or 22 μ m Wide Field Infrared Explorer surveys of the Galactic Plane as probable dusty interstellar bowshocks powered by early-type stars. About 20% are visible at 8 μ m or at shorter mid-infrared wavelengths. The vast majority (660) have no previous identification in the literature. These extended infrared sources are strongly concentrated near the Galactic mid-plane, with an angular scale height of ∼0.°6. All host a symmetrically placed star implicated as the source of a stellar wind sweeping up interstellar material. These are candidate “runaway” stars potentially having high velocities in the reference frame of the local medium. Among the 286 objects with measured proper motions, we find an unambiguous excess with velocity vectors aligned with the infrared morphology—kinematic evidence that many of these are “runaway” stars with large peculiar motions responsible for the bowshock signature. We discuss a population of “in situ” bowshocks (∼103 objects) that face giant H ii regions where the relative motions between the star and ISM may be caused by bulk outflows from an overpressured bubble. We also identify ∼58 objects that face 8 μ m bright-rimmed clouds and apparently constitute a sub-class of in situ bowshocks where the stellar wind interacts with a photoevaporative flow (PEF) from an eroding molecular cloud interface (i.e., “PEF bowshocks”). Orientations of the arcuate nebulae exhibit a correlation over small angular scales, indicating that external influences such as H ii regions are responsible for producing some bowshock nebulae. However, the vast majority of the nebulae in this sample appear to be isolated (499 objects) from obvious external influences.

  8. A Comprehensive Search for Stellar Bowshock Nebulae in the Milky Way: A Catalog of 709 Mid-infrared Selected Candidates (United States)

    Kobulnicky, Henry A.; Chick, William T.; Schurhammer, Danielle P.; Andrews, Julian E.; Povich, Matthew S.; Munari, Stephan A.; Olivier, Grace M.; Sorber, Rebecca L.; Wernke, Heather N.; Dale, Daniel A.; Dixon, Don M.


    We identify 709 arc-shaped mid-infrared nebula in 24 μm Spitzer Space Telescope or 22 μm Wide Field Infrared Explorer surveys of the Galactic Plane as probable dusty interstellar bowshocks powered by early-type stars. About 20% are visible at 8 μm or at shorter mid-infrared wavelengths. The vast majority (660) have no previous identification in the literature. These extended infrared sources are strongly concentrated near the Galactic mid-plane, with an angular scale height of ˜0.°6. All host a symmetrically placed star implicated as the source of a stellar wind sweeping up interstellar material. These are candidate “runaway” stars potentially having high velocities in the reference frame of the local medium. Among the 286 objects with measured proper motions, we find an unambiguous excess with velocity vectors aligned with the infrared morphology—kinematic evidence that many of these are “runaway” stars with large peculiar motions responsible for the bowshock signature. We discuss a population of “in situ” bowshocks (˜103 objects) that face giant H ii regions where the relative motions between the star and ISM may be caused by bulk outflows from an overpressured bubble. We also identify ˜58 objects that face 8 μm bright-rimmed clouds and apparently constitute a sub-class of in situ bowshocks where the stellar wind interacts with a photoevaporative flow (PEF) from an eroding molecular cloud interface (i.e., “PEF bowshocks”). Orientations of the arcuate nebulae exhibit a correlation over small angular scales, indicating that external influences such as H ii regions are responsible for producing some bowshock nebulae. However, the vast majority of the nebulae in this sample appear to be isolated (499 objects) from obvious external influences.

  9. Reciprocal responses of fibroblasts and melanocytes to α-MSH depending on MC1R polymorphisms. (United States)

    Stanisz, Hedwig; Seifert, Markus; Tilgen, Wolfgang; Vogt, Thomas; Rass, Knuth


    The melanocortin 1-receptor (MC1R) exhibits several variants in form of single nucleotide polymorphisms (SNPs) that are known to differentially regulate melanocyte function. However, whether and how MC1R polymorphisms also affect fibroblast function has not been investigated so far.Therefore we measured intracellular cyclic adenosine monophosphate (cAMP) concentrations and cellular proliferation upon stimulation with alpha-melanocyte stimulating hormone (α-MSH) in eight different human fibroblast and melanocyte cell lines with wild type and different MC1R SNPs.We found that fibroblasts, as well as melanocytes, show differences in MC1R function depending on the MC1R genotype. MC1R stimulation with α-MSH in wild type (MC1R(wt)) melanocytes results in an increase of intracellular cAMP and cellular proliferation. In contrast, MC1R(wt) fibroblasts react with a decrease of intracellular cAMP and proliferation. In MC1R polymorphic fibroblasts (R163Q, R151C and V60L) both effects are significantly alleviated. Similar, but inverse effects could be found in MC1R polymorphic melanocytes (R142H and V92M) with a significantly lower cAMP increase and proliferation rate compared to MC1R(wt) melanocytes.Our results indicate that the MC1R displays reciprocal growth responses in melanocytes and fibroblasts, depending on the MC1R genotype. Thus, the MC1R seems to be not solely important for the skin pigmentary system, but also for the fibroblast function, and might influence different processes of the dermal compartment like wound healing, fibrosis and keloid formation.

  10. Modeling PAH chemistry in the solar nebula (United States)

    Kress, M.; Tielens, A.; Frenklach, M.

    Polycyclic aromatic hydrocarbons (PAHs) are one of the predominant carriers of carbon in interstellar space, after CO. PAHs are also common in carbonaceous chondrites, which were likely an important source of organic carbon on the early Earth. Several outstanding questions in astrobiology address the relationship between the interstellar PAHs and those in meteorites: are meteoritic PAHs directly inherited from the interstellar medium? Are these compounds modified in the solar nebula, and if so, where and when? Can the abundances and varieties of PAHs in chondrites give more insight into the evolution of the solar nebula and other protoplanetary disks? To this end, we are modeling the chemical kinetics of PAH formation, growth and destruction within a parameter space of nebular timescales, pressures, temperatures, C/O ratios and other factors to begin to address these questions. The chemical kinetics of PAHs has been well studied at the higher temperatures present in plug flow reactors and flames (> 1000 K). We hope that our results will motivate more detailed studies of PAH chemistry at the lower temperatures characteristic of those in which meteorite parent bodies condensed (˜ a few hundred K). This work has been supported by NASA Astrobiology Institute's Virtual Planetary Laboratory and the Institute for Geophysics and Planetary Physics at Lawrence Livermore National Laboratory.

  11. Physics and chemistry of the solar nebula. (United States)

    Lunine, J I


    The solar system is thought to have begun in a flattened disk of gas and dust referred to traditionally as the solar nebula. Such a construct seems to be a natural product of the collapse of dense parts of giant molecular clouds, the vast star-forming regions that pepper the Milky Way and other galaxies. Gravitational, magnetic and thermal forces within the solar nebula forced a gradual evolution of mass toward the center (where the sun formed) and angular momentum (borne by a small fraction of the mass) toward the outer more distant regions of the disk. This evolution was accompanied by heating and a strong temperature contrast from the hot, inner regions to the cold, more remote parts of the disk. The resulting chemistry in the disk determined the initial distribution of organic matter in the planets; most of the reduced carbon species, in condensed form, were located beyond the asteroid belt (the 'outer' solar system). The Earth could have received much of its inventory of pre-biological material from comets and other icy fragments of the process of planetary formation in the outer solar system.

  12. Si isotope homogeneity of the solar nebula

    Energy Technology Data Exchange (ETDEWEB)

    Pringle, Emily A.; Savage, Paul S.; Moynier, Frédéric [Department of Earth and Planetary Sciences and McDonnell Center for the Space Sciences, Washington University in St. Louis, One Brookings Drive, St. Louis, MO 63130 (United States); Jackson, Matthew G. [Department of Earth Science, University of California, Santa Barbara, CA 93109 (United States); Barrat, Jean-Alix, E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [Université Européenne de Bretagne, Université de Brest, CNRS UMR 6538 (Domaines Océaniques), I.U.E.M., Place Nicolas Copernic, F-29280 Plouzané Cedex (France)


    The presence or absence of variations in the mass-independent abundances of Si isotopes in bulk meteorites provides important clues concerning the evolution of the early solar system. No Si isotopic anomalies have been found within the level of analytical precision of 15 ppm in {sup 29}Si/{sup 28}Si across a wide range of inner solar system materials, including terrestrial basalts, chondrites, and achondrites. A possible exception is the angrites, which may exhibit small excesses of {sup 29}Si. However, the general absence of anomalies suggests that primitive meteorites and differentiated planetesimals formed in a reservoir that was isotopically homogenous with respect to Si. Furthermore, the lack of resolvable anomalies in the calcium-aluminum-rich inclusion measured here suggests that any nucleosynthetic anomalies in Si isotopes were erased through mixing in the solar nebula prior to the formation of refractory solids. The homogeneity exhibited by Si isotopes may have implications for the distribution of Mg isotopes in the solar nebula. Based on supernova nucleosynthetic yield calculations, the expected magnitude of heavy-isotope overabundance is larger for Si than for Mg, suggesting that any potential Mg heterogeneity, if present, exists below the 15 ppm level.

  13. Dark nebulae, dark lanes, and dust belts

    CERN Document Server

    Cooke, Antony


    As probably the only book of its type, this work is aimed at the observer who wants to spend time with something less conventional than the usual fare. Because we usually see objects in space by means of illumination of one kind or another, it has become routine to see them only in these terms. However, part of almost everything that we see is the defining dimension of dark shading, or even the complete obscuration of entire regions in space. Thus this book is focused on everything dark in space: those dark voids in the stellar fabric that mystified astronomers of old; the dark lanes reported in many star clusters; the magical dust belts or dusty regions that have given so many galaxies their identities; the great swirling 'folds' that we associate with bright nebulae; the small dark feature detectable even in some planetary nebulae; and more. Many observers pay scant attention to dark objects and details. Perhaps they are insufficiently aware of them or of the viewing potential they hold, but also it may be...

  14. Nebular Spectroscopy: A Guide on Hii Regions and Planetary Nebulae (United States)

    Peimbert, Manuel; Peimbert, Antonio; Delgado-Inglada, Gloria


    We present a tutorial on the determination of the physical conditions and chemical abundances in gaseous nebulae. We also include a brief review of recent results on the study of gaseous nebulae, their relevance for the study of stellar evolution, galactic chemical evolution, and the evolution of the universe. One of the most important problems in abundance determinations is the existence of a discrepancy between the abundances determined with collisionally excited lines and those determined by recombination lines: this is called abundance discrepancy factor (ADF) problem, and we review results related to it. Finally, we discuss the possible reasons for the large t 2 values observed in gaseous nebulae.

  15. Extragalactic Planetary Nebulae Candidates Found with HST/LEGUS (United States)

    Kowalski, Laura; Churnetski, Kristen; Pellerin, Anne; Annibali, Francesca; LEGUS


    We present the results of an extensive search for planetary nebulae in nearby galaxies observed for the Legacy ExtraGalactic Ultraviolet Survey. A total of 32 galaxies were examined and 166 planetary nebula candidates were found. Images from the Hubble Space Telescope in ~BVI filters were visually inspected to identify potential candidates based on their color. The presence of strong emission lines from [OIII]4959, 5007A in planetary nebulae enhances their brightness in the F555W or F606W filter compared to other, more ordinary stars. Making use of the LEGUS stellar photometric catalogs, color-magnitude and color-color diagrams were used to eliminate outliers.

  16. Estimating the Binary Fraction of Central Stars of Planetary Nebulae (United States)

    Douchin, Dimitri


    Planetary nebulae are the end-products of intermediate-mass stars evolution, following a phase of expansion of their atmospheres at the end of their lives. Observationally, it has been estimated that 80% of them have non-spherical shapes. Such a high fraction is puzzling and has occupied the planetary nebula community for more than 30 years. One scenario that would allow to justify the observed shapes is that a comparable fraction of the progenitors of central stars of planetary nebula (CSPN) are not single, but possess a companion. The shape of the nebulae would then be the result of an interaction with this companion. The high fraction of non-spherical planetary nebulae would thus imply a high fraction of binary central stars of planetary nebulae, making binarity a preferred channel for planetary nebula formation. After presenting the current state of knowledge regarding planetary nebula formation and shaping and reviewing the diverse efforts to find binaries in planetary nebulae, I present my work to detect a near-infrared excess that would be the signature of the presence of cool companions. The first part of the project consists in the analysis of data and photometry acquired and conducted by myself. The second part details an attempt to make use of archived datasets: the Sloan Digital Sky Survey Data Release 7 optical survey and the extended database assembled by Frew (2008). I also present results from a radial velocity analysis of VLT/UVES spectra for 14 objects aiming to the detection of spectroscopic companions. Finally I give details of the analysis of optical photometry data from our observations associated to the detection of companions around central stars of planetary nebulae using the photometric variability technique. The main result of this thesis is from the near-infrared excess studies which I combine with previously published data. I conclude that if the detected red and NIR flux excess is indicative of a stellar companion then the binary

  17. Induced massive star formation in the trifid nebula? (United States)

    Cernicharo; Lefloch; Cox; Cesarsky; Esteban; Yusef-Zadeh; Mendez; Acosta-Pulido; Garcia Lopez RJ; Heras


    The Trifid nebula is a young (10(5) years) galactic HII region where several protostellar sources have been detected with the infrared space observatory. The sources are massive (17 to 60 solar masses) and are associated with molecular gas condensations at the edges or inside the nebula. They appear to be in an early evolutionary stage and may represent the most recent generation of stars in the Trifid. These sources range from dense, apparently still inactive cores to more evolved sources, undergoing violent mass ejection episodes, including a source that powers an optical jet. These observations suggest that the protostellar sources may have evolved by induced star formation in the Trifid nebula.

  18. The Kinematics of Massive Stars and Circumstellar Material in the Carina Nebula (United States)

    Kiminki, Megan Michelle

    This dissertation presents the results of three related projects, each focusing on an aspect of the massive stars in the Carina Nebula and how they impact their surroundings. First, I use the proper motions of dense gas ejected by eta Carinae to show that this luminous blue variable (LBV) has experienced major eruptions not just once but three times in the past millennium. The three eruptions show distinctly different symmetries: the thirteenth-century event was essentially one-sided, while the sixteenth-century event and the nineteenth-century Great Eruption were bipolar but not aligned with each other. These observations provide new constraints to theoretical models of eta Car and LBVs. In the second project, I constrain the proper motions of five other massive stars in the Carina Nebula. Each of these five has a stellar wind bow shock, but I find that none are runaway stars. In two cases, the bow shocks, which face a cluster that is driving large-scale flows of ionized gas, point at right angles to the motion of their stars. In the other three cases, both feedback-driven gas flows and stellar motion may be factors in setting bow shock orientation. The third section of this dissertation is a survey of the radial velocities of the Carina Nebula's full O-star population, combining new spectroscopy with a thorough review of values from the literature. The radial velocity distribution supports a common distance to the region's various clusters and subclusters. Comparison to molecular gas velocities shows that feedback from the Trumpler 16 cluster (home to eta Car), has accelerated a dense cloud toward us and possibly triggered additional massive-star formation. Comparison to ionized gas velocities shows that the feedback-driven expansion of the H II region is not spherical and is likely constrained by an unseen dense cloud on the far side.


    Energy Technology Data Exchange (ETDEWEB)

    Temim, Tea; Sonneborn, George; Dwek, Eli; Arendt, Richard G. [Observational Cosmology Lab, Code 665, NASA Goddard Space Flight Center, Greenbelt, MD 20771 (United States); Gehrz, Robert D. [Minnesota Institute for Astrophysics, University of Minnesota, 116 Church Street SE, Minneapolis, MN 55455 (United States); Slane, Patrick [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Roellig, Thomas L., E-mail: [NASA Ames Research Center, MS 245-6, Moffett Field, CA 94035-1000 (United States)


    Recent infrared (IR) observations of freshly formed dust in supernova remnants have yielded significantly lower dust masses than predicted by theoretical models and measured from high-redshift observations. The Crab Nebula's pulsar wind is thought to be sweeping up freshly formed supernova (SN) dust along with the ejected gas. The evidence for this dust was found in the form of an IR excess in the integrated spectrum of the Crab and in extinction against the synchrotron nebula that revealed the presence of dust in the filament cores. We present the first spatially resolved emission spectra of dust in the Crab Nebula acquired with the Infrared Spectrograph on board the Spitzer Space Telescope. The IR spectra are dominated by synchrotron emission and show forbidden line emission from S, Si, Ne, Ar, O, Fe, and Ni. We derived a synchrotron spectral map from the 3.6 and 4.5 {mu}m images, and subtracted this contribution from our data to produce a map of the residual continuum emission from dust. The dust emission appears to be concentrated along the ejecta filaments and is well described by an amorphous carbon or silicate grain compositions. We find a dust temperature of 55 {+-} 4 K for silicates and 60 {+-} 7 K for carbon grains. The total estimated dust mass is (1.2-12) Multiplication-Sign 10{sup -3} M{sub Sun }, well below the theoretical dust yield predicted for a core-collapse supernova. Our grain heating model implies that the dust grain radii are relatively small, unlike what is expected for dust grains formed in a Type IIP SN.

  20. Infrared Photometric Properties of 709 Candidate Stellar Bowshock Nebulae (United States)

    Kobulnicky, Henry A.; Schurhammer, Danielle P.; Baldwin, Daniel J.; Chick, William T.; Dixon, Don M.; Lee, Daniel; Povich, Matthew S.


    Arcuate infrared nebulae are ubiquitous throughout the Galactic Plane and are candidates for partial shells, bubbles, or bowshocks produced by massive runaway stars. We tabulate infrared photometry for 709 such objects using images from the Spitzer Space Telescope, the Wide-field Infrared Explorer, and the Herschel Space Observatory (HSO). Of the 709 objects identified at 24 or 22 μm, 422 are detected at the HSO 70 μm bandpass. Of these, only 39 are detected at HSO 160 μm. The 70 μm peak surface brightnesses are 0.5–2.5 Jy arcmin‑2. Color temperatures calculated from the 24 to 70 μm ratios range from 80 to 400 K. Color temperatures from 70 to 160 μm ratios are systematically lower, 40–200 K. Both of these temperature are, on average, 75% higher than the nominal temperatures derived by assuming that dust is in steady-state radiative equilibrium. This may be evidence of stellar wind bowshocks sweeping up and heating—possibly fragmenting but not destroying—interstellar dust. Infrared luminosity correlates with standoff distance, R 0, as predicted by published hydrodynamical models. Infrared spectral energy distributions are consistent with interstellar dust exposed to either single radiant energy density, U={10}3{--}{10}5 (in more than half of the objects) or a range of radiant energy densities U min = 25 to U max = 103–105 times the mean interstellar value for the remainder. Hence, the central OB stars dominate the energetics, making these enticing laboratories for testing dust models in constrained radiation environments. The spectral energy densities are consistent with polycyclic aromatic hydrocarbon fractions {q}{PAH}≲ 1 % in most objects.

  1. Discovery of a Circumstellar Disk in the Lagoon Nebula (United States)


    and barely visible on the HST-WFPC2 images taken at far-red optical wavelengths, is indeed situated behind the bright bow which is most conspicuous in the light of the red H-alpha spectral line, emitted by hydrogen atoms. The appearance of this object is thus similar to that of the proplyd sources found in the Orion Nebula. Caption to ESO PR Photo 10/97 [GIF, 296k] This is quite obvious from ESO Press Photo 10/97 which shows a colour composite based on HST-WFPC2 images obtained through narrow-band optical filtres, isolating the light of doubly ionized oxygen atoms ([OIII]; blue) and atomic hydrogen (H-alpha; green) and in a far-red band (red). Two more faint stars are seen in this image while the bright star Her 36 is outside the border of the image (its location is at the lower left, at the intersection of the vertical, saturated CCD column and the 45 o line caused by the light diffracted in the telescope). In contrast to the Orion Nebula, the non-uniform distribution of light-absorbing dust in the foreground makes the detection of the ionised tail difficult. Note that the image is rotated clockwise by 146 o with respect to the astronomical coordinate system. A proplyd in the Lagoon Nebula The detailed description of these results is the subject of a forthcoming research paper [5]. The new understanding of G5.97-1.17, i.e. as harbouring an evaporating circumstellar disk heated by far-ultraviolet radiation from Her 36, is supported by the fact that a sufficient amount of high-energy ultraviolet light is received from that star to account for the radio emission observed from the ionised bow. This object therefore represents the first proplyd-type object detected outside Orion at a much larger distance . The full description of this phenomenon requires detailed knowledge on the physical conditions of the star Her 36 and the object itself. Unfortunately, sofar little is known about the properties of the stellar wind from Her 36, the mass-loss rate from G5.97-1.17 and the

  2. Assembling the HST Carina Nebula Mosaic (United States)

    Levay, Zoltan G.; Smith, N.; Bond, H. E.; Christian, C. A.; Frattare, L. M.; Hamilton, F.; Januszewski, W.; Mutchler, M.; Knoll, K. S.


    Hubble Space Telescope has obtained numerous images of the Carina Nebula with ACS/WFC using the F658N filter (Hα+[N II]), revealing exquisite detail in this active star-forming region rich in finely detailed structure. Forty-eight overlapping fields were composited into a nearly contiguous mosaic of WFC pointings, resulting in a monochrome image of roughly 500 megapixels spanning ˜24'×12'. In addition, overlapping, wider-field images obtained with the CTIO 4m and MOSAIC2 camera in three narrow-band filters were combined into a color composite. We demonstrate a luminosity layering technique (LRGB) to reconstruct a high-resolution color image by combining the monochrome HST image with the color composite CTIO data which preserves the high spatial resolution brightness structure superimposed on the lower spatial resolution color values. We also touch on some cosmetic techniques to clean the image, including filling in small areas of data gaps and saturation.

  3. Spitzer IRS spectroscopy of planetary nebulae (United States)

    Ramos-Larios, G.; Guerrero, M. A.; Mata, H.; Fang, X.; Nigoche-Netro, A.; Toalá, J. A.; Rubio, G.


    We present Spitzer Space Telescope archival mid-infrared (mid-IR) spectroscopy of a sample of eleven planetary nebulae (PNe). The observations, acquired with the Spitzer Infrared Spectrograph (IRS), cover the spectral range 5.2-14.5 μm that includes the H2 0-0 S(2) to S(7) rotational emission lines. This wavelength coverage has allowed us to derive the Boltzmann distribution and calculate the H2 rotational excitation temperature (Tex). The derived excitation temperatures have consistent values ~= 900 +/-70 K for different sources despite their different structural components. We also report the detection of mid-IR ionic lines of [Ar III], [S IV], and [Ne II] in most objects, and polycyclic aromatic hydrocarbon (PAH) features in a few cases.

  4. ISO Spectroscopy of Proto-Planetary Nebulae (United States)

    Hrivnak, Bruce J.


    The goal of this program was to determine the chemical properties of the dust shells around protoplanetary nebulae (PPNs) through a study of their short-wavelength (6-45 micron) infrared spectra. PPNs are evolved stars in transition from the asymptotic giant branch to the planetary nebula stages. Spectral features in the 10 to 20 gm region indicate the chemical nature (oxygen- or carbon-rich), and the strengths of the features relate to the physical properties of the shells. A few bright carbon-rich PPNs have been observed to show PAH features and an unidentified 21 micron emission feature. We used the Infrared Space Observatory (ISO) to observe a sample of IRAS sources that have the expected properties of PPNs and for which we have accurate positions. Some of these have optical counterparts (proposal SWSPPN01) and some do not (SWSPPN02). We had previously observed these from the ground with near-infrared photometry and, for those with visible counterparts, visible photometry and spectroscopy, which we have combined with these new ISO data in the interpretation of the spectra. We have completed a study of the unidentified emission feature at 21 micron in eight sources. We find the shape of the feature to be the same in all of the sources, with no evidence of any substructure. The ratio of the emission peak to continuum ranges from 0.13 to 1.30. We have completed a study of seven PPNs and two other carbon-rich objects for which we had obtained ISO 2-45 micron observations. The unidentified emission features at 21 and 30 micron were detected in six sources, including four new detections of the 30 micron feature. This previously unresolved 30 micron feature was resolved and found to consist of a broad feature peaking at 27.2 micron (the "30 micron" feature) and a narrower feature peaking at 25.5 micron (the "26 micron" feature). This new 26 micron feature is detected in eight sources and is particularly strong in IRAS Z02229+6208 and 16594-4656. The unidentified

  5. Distinguishing between symbiotic stars and planetary nebulae (United States)

    Iłkiewicz, K.; Mikołajewska, J.


    Context. The number of known symbiotic stars (SySt) is still significantly lower than their predicted population. One of the main problems in finding the total population of SySt is the fact that their spectrum can be confused with other objects, such as planetary nebulae (PNe) or dense H II regions. This problem is reinforced by the fact that in a significant fraction of established SySt the emission lines used to distinguish them from other objects are not present. Aims: We aim at finding new diagnostic diagrams that could help separate SySt from PNe. Additionally, we examine a known sample of extragalactic PNe for candidate SySt. Methods: We employed emission line fluxes of known SySt and PNe from the literature. Results: We found that among the forbidden lines in the optical region of spectrum, only the [O III] and [N II] lines can be used as a tool for distinguishing between SySt and PNe, which is consistent with the fact that they have the highest critical densities. The most useful diagnostic that we propose is based on He I lines, which are more common and stronger in SySt than forbidden lines. All these useful diagnostic diagrams are electron density indicators that better distinguish PNe and ionized symbiotic nebulae. Moreover, we found six new candidate SySt in the Large Magellanic Cloud and one in M 81. If confirmed, the candidate in M 81 would be the farthest known SySt thus far.

  6. Watching Young Planetary Nebulae Grow: The Movie (United States)

    Balick, Bruce


    The development of magneto-hydro gas dynamical models is the key to the understanding of both the physics {processes} and astronomy {initial conditions} of astrophysical nebulae of all sorts. The models are reaching their highest degree of accuracy when applied to and compared against pre Planetary Nebulae {pPNe} thanks to the simplicity, relative lack of extinction, and the detail of the imaging and kinematic data that have bcome available for these objects. The primary barrier to progress is inadequate kinematic data of pPNe against which the predictions models can be tested. Unlike PNe, pPNe do not emit emission lines for detailed Doppler measurements. Therefore it is essential to find another way to monitor the morphological evolution. Only HST can uncover the dynamics of the growth patterns by subtracting multi-epoch images spanning a decade or more. We have selected four pPNe with highly collimated outflows in different evolutionary stages for which high-quality first-epoch images were obtained from 1996 to 2002. All of them display regularly shaped thin rims, sharp edges, and symmetric pairs of knots or bowshocks that are ideal for our purposes. We will closely mimic many of the earlier exposures using ACS and to monitor changes in structures. The morphology and its evolution will be compared to 3-D MHD models with adaptive grids in order to build a far clearer picture of the nuclear geometry which shaped the outflows and constrained their propagation to the present. We shall also obtain R, J, and H images for use with a 3-D dust radiative transfer code LELUYA to model the dust distribution deep into the nuclear zones.

  7. Complex molecules in the Orion Kleinmann-Low nebula


    Despois D.; Brouillet N.; Peng T.-C.; Baudry A.; Favre C; Combes F.; Wlodarczak G.; Guélin M.


    In the framework of the delivery to the early Earth of extraterrestrial molecules, we have studied complex molecular species toward the Orion Kleinmann-Low nebula. This nebula is rich in molecules as well as in nascent stars and planetary systems. We focus here on HCOOCH3, CH3OCH3 and deuterated methanol. Upper limits on species of prebiotic interest like glycine were also obtained.


    Energy Technology Data Exchange (ETDEWEB)

    Yoshida, Michitoshi [Hiroshima Astrophysical Science Center, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526 (Japan); Yagi, Masafumi; Komiyama, Yutaka; Kashikawa, Nobunari [Optical and Infrared Astronomy Division, National Astronomical Observatory, Mitaka, Tokyo 181-8588 (Japan); Ohyama, Youichi [Institute of Astronomy and Astrophysics, Academia Sinica, 11F of Astronomy-Mathematics Building, AS/NTU No.1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, R.O.C. (China); Tanaka, Hisashi [Department of Physical Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526 (Japan); Okamura, Sadanori, E-mail: [Department of Advanced Sciences, Faculty of Science and Engineering, Hosei University, Koganei, Tokyo 184-8584 (Japan)


    We revealed the detailed structure of a vastly extended Hα-emitting nebula (“Hα nebula”) surrounding the starburst/merging galaxy NGC 6240 by deep narrow-band imaging observations with the Subaru Suprime-Cam. The extent of the nebula is ∼90 kpc in diameter and the total Hα luminosity amounts to L{sub Hα} ≈ 1.6 × 10{sup 42} erg s{sup −1}. The volume filling factor and the mass of the warm ionized gas are ∼10{sup −4}–10{sup −5} and ∼5 × 10{sup 8} M{sub ⊙}, respectively. The nebula has a complicated structure, which includes numerous filaments, loops, bubbles, and knots. We found that there is a tight spatial correlation between the Hα nebula and the extended soft-X-ray-emitting gas, both in large and small scales. The overall morphology of the nebula is dominated by filamentary structures radially extending from the center of the galaxy. A large-scale bipolar bubble extends along the minor axis of the main stellar disk. The morphology strongly suggests that the nebula was formed by intense outflows—superwinds—driven by starbursts. We also found three bright knots embedded in a looped filament of ionized gas that show head-tail morphologies in both emission-line and continuum, suggesting close interactions between the outflows and star-forming regions. Based on the morphology and surface brightness distribution of the Hα nebula, we propose the scenario that three major episodes of starburst/superwind activities, which were initiated ∼10{sup 2} Myr ago, formed the extended ionized gas nebula of NGC 6240.

  9. Complex molecules in the Orion Kleinmann-Low nebula

    Directory of Open Access Journals (Sweden)

    Despois D.


    Full Text Available In the framework of the delivery to the early Earth of extraterrestrial molecules, we have studied complex molecular species toward the Orion Kleinmann-Low nebula. This nebula is rich in molecules as well as in nascent stars and planetary systems. We focus here on HCOOCH3, CH3OCH3 and deuterated methanol. Upper limits on species of prebiotic interest like glycine were also obtained.

  10. Beneficial effects of the melanocortin analogue Nle4-D-Phe7-α-MSH in acne vulgaris. (United States)

    Böhm, M; Ehrchen, J; Luger, T A


    α-Melanocyte-stimulating hormone (α-MSH) is a melanocortin peptide that increases skin pigmentation during ultraviolet light-mediated tanning. As α-MSH has been shown to possess anti-inflammatory effects, we assessed the clinical potential of a superpotent α-MSH analogue, afamelanotide (Nle(4)-D-Phe(7)-α-MSH), in patients with acne vulgaris, the most common inflammatory skin disorder. Afamelanotide (16 mg) was given in a phase II open-label pilot study subcutaneously as a sustained-release resorbable implant formulation to 3 patients with mild-to-moderate facial acne vulgaris. Evaluation included lesion count, adverse effects and patient-reported outcome. Monitoring of laboratory parameters included differential blood counts, electrolytes, urine analysis, and liver and kidney function tests. Skin melanin density was measured by reflectance spectrophotometry. The total number as well as the number of inflammatory acne lesions declined in all patients 56 days after the first injection of afamelanotide. Life quality as measured by Dermatology Life Quality Index likewise improved in all 3 patients 56 days after the first injection of afamelanotide. There were no adverse effects except mild and short-term fatigue in one patient. All patients experienced increased pigmentation especially on the face. Clinically relevant changes in laboratory parameters were not detected. Afamelanotide appears to have anti-inflammatory effects in patients with mild-to-moderate acne vulgaris. Future trials are needed to confirm the anti-inflammatory action of this melanocortin analogue in patients with acne vulgaris. © 2012 The Authors. Journal of the European Academy of Dermatology and Venereology © 2012 European Academy of Dermatology and Venereology.

  11. HAWC Analysis of the Crab Nebula Using Neural-Net Energy Reconstruction (United States)

    Marinelli, Samuel; HAWC Collaboration


    The HAWC (High-Altitude Water-Cherenkov) experiment is a TeV γ-ray observatory located 4100 m above sea level on the Sierra Negra mountain in Puebla, Mexico. The detector consists of 300 water-filled tanks, each instrumented with 4 photomuliplier tubes that utilize the water-Cherenkov technique to detect atmospheric air showers produced by cosmic γ rays. Construction of HAWC was completed in March, 2015. The experiment's wide field of view (2 sr) and high duty cycle (> 95 %) make it a powerful survey instrument sensitive to pulsar wind nebulae, supernova remnants, active galactic nuclei, and other γ-ray sources. The mechanisms of particle acceleration at these sources can be studied by analyzing their energy spectra. To this end, we have developed an event-by-event energy-reconstruction algorithm employing an artificial neural network to estimate energies of primary γ rays. The Crab Nebula, the brightest source of TeV photons, makes an excellent calibration source for this technique. We will present preliminary results from an analysis of the Crab energy spectrum using this new energy-reconstruction method. This work was supported by the National Science Foundation.

  12. Design of MC1R Selective γ-MSH Analogues with Canonical Amino Acids Leads to Potency and Pigmentation. (United States)

    Zhou, Yang; Mowlazadeh Haghighi, Saghar; Zoi, Ioanna; Sawyer, Jonathon R; Hruby, Victor J; Cai, Minying


    Melanoma is a lethal form of skin cancer. Skin pigmentation, which is regulated by the melanocortin 1 receptor (MC1R), is an effective protection against melanoma. However, the endogenous MC1R agonists lack selectivity for the MC1R and thus can have side effects. The use of noncanonical amino acids in previous MC1R ligand development raises safety concerns. Here we report the development of the first potent and selective hMC1R agonist with only canonical amino acids. Using γ-MSH as a template, we developed a peptide, [Leu3, Leu7, Phe8]-γ-MSH-NH2 (compound 5), which is 16-fold selective for the hMC1R (EC50 = 4.5 nM) versus other melanocortin receptors. Conformational studies revealed a constrained conformation for this linear peptide. Molecular docking demonstrated a hydrophobic binding pocket for the melanocortin 1 receptor. In vivo pigmentation study shows high potency and short duration. [Leu3, Leu7, Phe8]-γ-MSH-NH2 is ideal for inducing short-term skin pigmentation without sun for melanoma prevention.

  13. Regulation of Melanopsins and Per1 by α-MSH and Melatonin in Photosensitive Xenopus laevis Melanophores

    Directory of Open Access Journals (Sweden)

    Maria Nathália de Carvalho Magalhães Moraes


    Full Text Available α-MSH and light exert a dispersing effect on pigment granules of Xenopus laevis melanophores; however, the intracellular signaling pathways are different. Melatonin, a hormone that functions as an internal signal of darkness for the organism, has opposite effects, aggregating the melanin granules. Because light functions as an important synchronizing signal for circadian rhythms, we further investigated the effects of both hormones on genes related to the circadian system, namely, Per1 (one of the clock genes and the melanopsins, Opn4x and Opn4m (photopigments. Per1 showed temporal oscillations, regardless of the presence of melatonin or α-MSH, which slightly inhibited its expression. Melatonin effects on melanopsins depend on the time of application: if applied in the photophase it dramatically decreased Opn4x and Opn4m expressions, and abolished their temporal oscillations, opposite to α-MSH, which increased the melanopsins’ expressions. Our results demonstrate that unlike what has been reported for other peripheral clocks and cultured cells, medium changes or hormones do not play a major role in synchronizing the Xenopus melanophore population. This difference is probably due to the fact that X. laevis melanophores possess functional photopigments (melanopsins that enable these cells to primarily respond to light, which triggers melanin dispersion and modulates gene expression.

  14. α-MSH regulates intergenic splicing of MC1R and TUBB3 in human melanocytes (United States)

    Dalziel, Martin; Kolesnichenko, Marina; das Neves, Ricardo Pires; Iborra, Francisco; Goding, Colin; Furger, André


    Alternative splicing enables higher eukaryotes to increase their repertoire of proteins derived from a restricted number of genes. However, the possibility that functional diversity may also be augmented by splicing between adjacent genes has been largely neglected. Here, we show that the human melanocortin 1 receptor (MC1R) gene, a critical component of the facultative skin pigmentation system, has a highly complex and inefficient poly(A) site which is instrumental in allowing intergenic splicing between this locus and its immediate downstream neighbour tubulin-β-III (TUBB3). These transcripts, which produce two distinct protein isoforms localizing to the plasma membrane and the endoplasmic reticulum, seem to be restricted to humans as no detectable chimeric mRNA could be found in MC1R expressing mouse melanocytes. Significantly, treatment with the MC1R agonist α-MSH or activation of the stress response kinase p38-MAPK, both key molecules associated with ultraviolet radiation dermal insult and subsequent skin tanning, result in a shift in expression from MC1R in favour of chimeric MC1R-TUBB3 isoforms in cultured melanocytes. We propose that these chimeric proteins serve to equip melanocytes with novel cellular phenotypes required as part of the pigmentation response. PMID:21071418

  15. Chick subcutaneous and abdominal adipose tissue depots respond differently in lipolytic and adipogenic activity to α-melanocyte stimulating hormone (α-MSH). (United States)

    Shipp, Steven L; Wang, Guoqing; Cline, Mark A; Gilbert, Elizabeth R


    In birds, α-MSH is anorexigenic, but effects on adipose tissue are unknown. Four day-old chicks were intraperitoneally injected with 0 (vehicle), 5, 10, or 50μg of α-MSH and subcutaneous and abdominal adipose tissue collected at 60min for RNA isolation (n=10). Plasma was collected post-euthanasia at 60 and 180min for measuring non-esterified fatty acids (NEFA) and α-MSH (n=10). Relative to the vehicle, food intake was reduced in the 50μg-treated group. Plasma NEFAs were greater in 10μg than vehicle-treated chicks at 3h. Plasma α-MSH was 3.06±0.57ng/ml. In subcutaneous tissue, melanocortin receptor 5 (MC5R) mRNA was increased in 10μg, MC2R and CCAAT-enhancer-binding protein β (C/EBPβ) mRNAs increased in 50μg, peroxisome proliferator-activated receptor γ and C/EBPα decreased in 5, 10 and 50μg, and Ki67 mRNA decreased in 50μg α-MSH-injected chicks, compared to vehicle-injected chicks. In abdominal tissue, adipose triglyceride lipase mRNA was greater in 10μg α-MSH- than vehicle-treated chicks. Cells isolated from abdominal fat that were treated with 10 and 100nM α-MSH for 4h expressed more MC5R and perilipin-1 than control cells (n=6). Cells that received 100nM α-MSH expressed more fatty acid binding protein 4 and comparative gene identification-58 mRNA than control cells. Glycerol-3-phosphate dehydrogenase (G3PDH) activity was greater in cells at 9days post-differentiation that were treated with 1 and 100nM α-MSH for 4h than in control cells (n=3). Results suggest that α-MSH increases lipolysis and reduces adipogenesis in adipose tissue. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. The carbon budget in the outer solar nebula. (United States)

    Simonelli, D P; Pollack, J B; McKay, C P; Reynolds, R T; Summers, A L


    Detailed models of the internal structures of Pluto and Charon, assuming rock and water ice as the only constituents, indicate that the mean silicate mass fraction of this two-body system is on the order of 0.7; thus the Pluto/Charon system is significantly "rockier" than the satellites of the giant planets (silicate mass fraction approximately 0.55). This compositional contrast reflects different formation mechanisms: it is likely that Pluto and Charon formed directly from the solar nebula, while the circumplanetary nebulae that produced the giant planet satellites were derived from envelopes that surrounded the forming giant planets (envelopes in which icy planetesimals dissolved more readily than rocky planetesimals). Simple cosmic abundance calculations, and the assumption that the Pluto/Charon system formed directly from solar nebula condensates, strongly suggest that the majority of the carbon in the outer solar nebula was in the form of carbon monoxide; these results are consistent with (1) inheritance from the dense molecular clouds in the interstellar medium (where CH4/CO nebula chemistry. Theoretical predictions of the C/H enhancements in the atmospheres of the giant planets, when compared to the actual observed enhancements, suggest that 10%, or slightly more, of the carbon in the outer solar nebula was in the form of condensed materials (although the amount of condensed C may have dropped slightly with increasing heliocentric distance). Strict compositional limits computed for the Pluto/Charon system using the densities of CH4 and CO ices indicate that these pure ices are at best minor components in the interiors of these bodies, and imply that CH4 and CO ices were not the dominant C-bearing solids in the outer nebula. Clathrate-hydrates could not have appropriated enough CH4 or CO to be the major form of condensed carbon, although such clathrates may be necessary to explain the presence of methane on Pluto after its formation from a CO-rich nebula

  17. Detail of the star WR124 and the surrounding nebula M1-67. (United States)


    The massive, hot central star is known as a Wolf-Rayet star. This extremely rare and short-lived class of super-hot star is going through a violent, transitional phase characterized by the fierce ejection of mass. The blobs may result from the furious stellar wind that does not flow smoothly into space but has instabilities which make it clumpy. This black and white image was made in the light of atomic hydrogen. The contrast has been increased to emphasize the fine detail in the nebula near the central star. Credit: Yves Grosdidier (University of Montreal and Observatoire de Strasbourg), Anthony Moffat (Universitie de Montreal), Gilles Joncas (Universite Laval), Agnes Acker (Observatoire de Strasbourg), and NASA

  18. Existence of an 16O-rich gaseous reservoir in the solar nebula. (United States)

    Krot, Alexander N; McKeegan, Kevin D; Leshin, Laurie A; MacPherson, Glenn J; Scott, Edward R D


    Carbonaceous chondrite condensate olivine grains from two distinct petrographic settings, calcium-aluminum-rich inclusion (CAI) accretionary rims and amoeboid olivine aggregates (AOAs), are oxygen-16 (16O) enriched at the level previously observed inside CAIs. This requires that the gas in the nebular region where these grains condensed was 16O-rich. This contrasts with an 16O-poor gas present during the formation of chondrules, suggesting that CAIs and AOAs formed in a spatially restricted region of the solar nebula containing 16O-rich gas. The 16O-rich gas composition may have resulted either from mass-independent isotopic chemistry or from evaporation of regions with enhanced dust/gas ratios, possibly in an X-wind environment near the young Sun.

  19. Characterizing global expansion evolution of the ionized matter in planetary nebulae (United States)

    López, J. A.; Richer, M. G.; Pereyra, M.; García-Díaz, M. T.


    Bulk outflow or global expansion velocities are presented for a large number of planetary nebulae (PNe) that span a wide range of evolutionary stages and different stellar populations. The sample comprises 133 PNe from the Galactic bulge, 100 mature and highly evolved PNe from the disk, 11 PNe from the Galactic halo and 15 PNe with very low central star masses and low metallicities, for a total of 259 PNe. These results reveal from a statistical perspective the kinematic evolution of the expansion velocities of PNe in relation to changing characteristics of the central star's wind and ionizing luminosity and as a function of the evolutionary rate determined by the central (CS) mass. The large number of PNe utilized in this work for each group of PNe under study and the homogeneity of the data provide for the first time a solid benchmark form observations for model predictions, as has been described by López et al. (2016).

  20. Observations of the Crab Nebula with the Chandra X-Ray Observatory (United States)

    Weisskopf, Martin C.


    The Crab Nebula and its pulsar has been the subject of a number of detailed observations with the Chandra X-ray Observatory. The superb angular resolution of Chandra s high-resolution telescope has made possible numerous remarkable results. Here we describe a number of specific studies of the Crab that I and my colleagues have undertaken. We discuss the geometry of the system, which indicates that the "inner X-ray ring", typically identified with the termination shock of the pulsar s particle wind, is most likely not in the equatorial plane of the pulsar. Other topics are the northern wisps and their evolution with time; the characterization of features in the jet to the southeast; pulse-phase spectroscopy and possible correlations with the features at other wavelengths, particularly the optical polarization; and a search for correlations of the X-ray flux with the recently-discovered gamma -ray flares.

  1. Signatures of Chemical Evolution in Protostellar Nebulae (United States)

    Nuth, Joseph A., III; Johnson, Natasha


    A decade ago observers began to take serious notice of the presence of crystalline silicate grains in the dust flowing away from some comets. While crystallinity had been seen in such objects previously, starting with the recognitions by Campins and Ryan (1990) that the 10 micron feature of Comet Halley resembled that of the mineral forsterite, most such observations were either ignored or dismissed as no path to explain such crystalline grains was available in the literature. When it was first suggested that an outward flow must be present to carry annealed silicate grains from the innermost regions of the Solar Nebula out to the regions where comets could form (Nuth, 1999; 2001) this suggestion was also dismissed because no such transport mechanism was known at the time. Since then not only have new models of nebular dynamics demonstrated the reality of long distance outward transport (Ciesla, 2007; 2008; 2009) but examination of older models (Boss, 2004) showed that such transport had been present but had gone unrecognized for many years. The most unassailable evidence for outward nebular transport came with the return of the Stardust samples from Comet Wild2, a Kuiper-belt comet that contained micron-scale grains of high temperature minerals resembling the Calcium-Aluminum Inclusions found in primitive meteorites (Zolensky et aI., 2006) that formed at T > 1400K. Now that outward transport in protostellar nebulae has been firmly established, a re-examination of its consequences for nebular gas is in order that takes into account both the factors that regulate both the outward flow as well as those that likely control the chemical composition of the gas. Laboratory studies of surface catalyzed reactions suggest that a trend toward more highly reduced carbon and nitrogen compounds in the gas phase should be correlated with a general increase in the crystallinity of the dust (Nuth et aI., 2000), but is such a trend actually observable? Unlike the Fischer-Tropsch or

  2. Bacterial ClpB heat-shock protein, an antigen-mimetic of the anorexigenic peptide α-MSH, at the origin of eating disorders. (United States)

    Tennoune, N; Chan, P; Breton, J; Legrand, R; Chabane, Y N; Akkermann, K; Järv, A; Ouelaa, W; Takagi, K; Ghouzali, I; Francois, M; Lucas, N; Bole-Feysot, C; Pestel-Caron, M; do Rego, J-C; Vaudry, D; Harro, J; Dé, E; Déchelotte, P; Fetissov, S O


    The molecular mechanisms at the origin of eating disorders (EDs), including anorexia nervosa (AN), bulimia and binge-eating disorder (BED), are currently unknown. Previous data indicated that immunoglobulins (Igs) or autoantibodies (auto-Abs) reactive with α-melanocyte-stimulating hormone (α-MSH) are involved in regulation of feeding and emotion; however, the origin of such auto-Abs is unknown. Here, using proteomics, we identified ClpB heat-shock disaggregation chaperone protein of commensal gut bacteria Escherichia coli as a conformational antigen mimetic of α-MSH. We show that ClpB-immunized mice produce anti-ClpB IgG crossreactive with α-MSH, influencing food intake, body weight, anxiety and melanocortin receptor 4 signaling. Furthermore, chronic intragastric delivery of E. coli in mice decreased food intake and stimulated formation of ClpB- and α-MSH-reactive antibodies, while ClpB-deficient E. coli did not affect food intake or antibody levels. Finally, we show that plasma levels of anti-ClpB IgG crossreactive with α-MSH are increased in patients with AN, bulimia and BED, and that the ED Inventory-2 scores in ED patients correlate with anti-ClpB IgG and IgM, which is similar to our previous findings for α-MSH auto-Abs. In conclusion, this work shows that the bacterial ClpB protein, which is present in several commensal and pathogenic microorganisms, can be responsible for the production of auto-Abs crossreactive with α-MSH, associated with altered feeding and emotion in humans with ED. Our data suggest that ClpB-expressing gut microorganisms might be involved in the etiology of EDs.

  3. DHFR/MSH3 amplification in methotrexate-resistant cells alters the hMutSα/hMutSβ ratio and reduces the efficiency of base–base mismatch repair (United States)

    Drummond, James T.; Genschel, Jochen; Wolf, Elisabeth; Modrich, Paul


    The level and fate of hMSH3 (human MutS homolog 3) were examined in the promyelocytic leukemia cell line HL-60 and its methotrexate-resistant derivative HL-60R, which is drug resistant by virtue of an amplification event that spans the dihydrofolate reductase (DHFR) and MSH3 genes. Nuclear extracts from HL-60 and HL-60R cells were subjected to an identical, rapid purification protocol that efficiently captures heterodimeric hMutSα (hMSH2⋅hMSH6) and hMutSβ (hMSH2⋅hMSH3). In HL-60 extracts the hMutSα to hMutSβ ratio is roughly 6:1, whereas in methotrexate-resistant HL-60R cells the ratio is less than 1:100, due to overproduction of hMSH3 and heterodimer formation of this protein with virtually all the nuclear hMSH2. This shift is associated with marked reduction in the efficiency of base–base mismatch and hypermutability at the hypoxanthine phosphoribosyltransferase (HPRT) locus. Purified hMutSα and hMutSβ display partial overlap in mismatch repair specificity: both participate in repair of a dinucleotide insertion–deletion heterology, but only hMutSα restores base–base mismatch repair to extracts of HL-60R cells or hMSH2-deficient LoVo colorectal tumor cells. PMID:9294177

  4. The Rapid Evolution of the Exciting Star of the Stingray Nebula (United States)

    Reindl, N.; Rauch, T.; Parthasarathy, M.; Werner, K.; Kruk, J.W.; Hamann, W. R.; Sander, A.; Todt, H.


    Context: SAO244567, the exciting star of the Stingray nebula, is rapidly evolving. Previous analyses suggested that it has heated up from an effective temperature of about 21 kK in 1971 to over 50 kK in the 1990s. Canonical post-asymptotic giant branch evolution suggests a relatively high mass while previous analyses indicate a low-mass star. Aims: A comprehensive model-atmosphere analysis of UV and optical spectra taken during 1988-2006 should reveal the detailed temporal evolution of its atmospheric parameters and provide explanations for the unusually fast evolution. Methods: Fitting line profiles from static and expanding non-LTE model atmospheres to the observed spectra allowed us to study the temporal change of effective temperature, surface gravity, mass-loss rate, and terminal wind velocity. In addition, we determined the chemical composition of the atmosphere. Results: We find that the central star has steadily increased its effective temperature from 38 kK in 1988 to a peak value of 60 kK in 2002. During the same time, the star was contracting, as concluded from an increase in surface gravity from log g = 4.8 to 6.0 and a drop in luminosity. Simultaneously, the mass-loss rate declined from log(M/M (solar mass) yr (exp -1)) = -9.0 to -11.6 and the terminal wind velocity increased from v (infinity) = 1800 km s (exp -1) to 2800 km s (exp -1). Since around 2002, the star stopped heating and has cooled down again to 55 kK by 2006. It has a largely solar surface composition with the exception of slightly subsolar carbon, phosphorus, and sulfur. The results are discussed by considering different evolutionary scenarios. Conclusions: The position of SAO244567 in the log T (sub eff) -log g plane places the star in the region of sdO stars. By comparison with stellar-evolution calculations, we confirm that SAO244567 must be a low-mass star (M nebula with a kinematical age of only about 1000 years. We speculate that the star could be a late He-shell flash object

  5. [WN] central stars of planetary nebulae (United States)

    Todt, H.; Miszalski, B.; Toalá, J. A.; Guerrero, M. A.


    While most of the low-mass stars stay hydrogen-rich on their surface throughout their evolution, a considerable fraction of white dwarfs as well as central stars of planetary nebulae have a hydrogen-deficient surface composition. The majority of these H-deficient central stars exhibit spectra very similar to massive Wolf-Rayet stars of the carbon sequence, i.e. with broad emission lines of carbon, helium, and oxygen. In analogy to the massive Wolf-Rayet stars, they are classified as [WC] stars. Their formation, which is relatively well understood, is thought to be the result of a (very) late thermal pulse of the helium burning shell. It is therefore surprising that some H-deficient central stars which have been found recently, e.g. IC 4663 and Abell 48, exhibit spectra that resemble those of the massive Wolf-Rayet stars of the nitrogen sequence, i.e. with strong emission lines of nitrogen instead of carbon. This new type of central stars is therefore labelled [WN]. We present spectral analyses of these objects and discuss the status of further candidates as well as the evolutionary status and origin of the [WN] stars.

  6. Planetary Nebulae and How to Observe Them

    CERN Document Server

    Griffiths, Martin


    Astronomers' Observing Guides provide up-to-date information for amateur astronomers who want to know all about what is it they are observing. This is the basis of the first part of the book. The second part details observing techniques for practical astronomers, working with a range of different instruments. Planetary Nebulae and How to Observe Them is intended for amateur astronomers who want to concentrate on one of the most beautiful classes of astronomical objects in the sky. This book will help the observer to see these celestial phenomena using telescopes of various apertures. As a Sun-like star reaches the end of its life, its hydrogen fuel starts to run out. It collapses until helium nuclei begin nuclear fusion, whereupon the star begins to pulsate, each pulsation throwing off a layer of the star's atmosphere. Eventually the atmosphere has all been ejected as an expanding cloud of gas, the star's core is exposed and ultraviolet photons cause the shell of gas to glow brilliantly - that's planetary ...

  7. Scaled Eagle Nebula Experiments on NIF

    Energy Technology Data Exchange (ETDEWEB)

    Pound, Marc W. [Univ. of Maryland, College Park, MD (United States)


    We performed scaled laboratory experiments at the National Ignition Facility laser to assess models for the creation of pillar structures in star-forming clouds of molecular hydrogen, in particular the famous Pillars of the Eagle Nebula. Because pillars typically point towards nearby bright ultraviolet stars, sustained directional illumination appears to be critical to pillar formation. The experiments mock up illumination from a cluster of ultraviolet-emitting stars, using a novel long duration (30--60 ns), directional, laser-driven x-ray source consisting of multiple radiation cavities illuminated in series. Our pillar models are assessed using the morphology of the Eagle Pillars observed with the Hubble Space Telescope, and measurements of column density and velocity in Eagle Pillar II obtained at the BIMA and CARMA millimeter wave facilities. In the first experiments we assess a shielding model for pillar formation. The experimental data suggest that a shielding pillar can match the observed morphology of Eagle Pillar II, and the observed Pillar II column density and velocity, if augmented by late time cometary growth.

  8. Accounting for planet-shaped planetary nebulae (United States)

    Sabach, Efrat; Soker, Noam


    By following the evolution of several observed exoplanetary systems, we show that by lowering the mass-loss rate of single solar-like stars during their two giant branches, these stars will swallow their planets at the tip of their asymptotic giant branch (AGB) phase. This will most likely lead the stars to form elliptical planetary nebulae (PNe). Under the traditional mass-loss rate these stars will hardly form observable PNe. Stars with a lower mass-loss rate as we propose, about 15 per cent of the traditional mass-loss rate of single stars, leave the AGB with much higher luminosities than what traditional evolution produces. Hence, the assumed lower mass-loss rate might also account for the presence of bright PNe in old stellar populations. We present the evolution of four exoplanetary systems that represent stellar masses in the range of 0.9-1.3 M⊙. The justification for this low mass-loss rate is our assumption that the stellar samples that were used to derive the traditional average single-star mass-loss rate were contaminated by stars that suffer binary interaction.

  9. Gamma rays and neutrinos from the Crab Nebula produced by pulsar accelerated nuclei


    Bednarek, W.; Protheroe, R. J.


    We investigate the consequences of the acceleration of heavy nuclei (e.g. iron nuclei) by the Crab pulsar. Accelerated nuclei can photodisintegrate in collisions with soft photons produced in the pulsar's outer gap, injecting energetic neutrons which decay either inside or outside the Crab Nebula. The protons from neutron decay inside the nebula are trapped by the Crab Nebula magnetic field, and accumulate inside the nebula producing gamma-rays and neutrinos in collisions with the matter in t...

  10. Association of a novel point mutation in MSH2 gene with familial multiple primary cancers

    Directory of Open Access Journals (Sweden)

    Hai Hu


    Full Text Available Abstract Background Multiple primary cancers (MPC have been identified as two or more cancers without any subordinate relationship that occur either simultaneously or metachronously in the same or different organs of an individual. Lynch syndrome is an autosomal dominant genetic disorder that increases the risk of many types of cancers. Lynch syndrome patients who suffer more than two cancers can also be considered as MPC; patients of this kind provide unique resources to learn how genetic mutation causes MPC in different tissues. Methods We performed a whole genome sequencing on blood cells and two tumor samples of a Lynch syndrome patient who was diagnosed with five primary cancers. The mutational landscape of the tumors, including somatic point mutations and copy number alternations, was characterized. We also compared Lynch syndrome with sporadic cancers and proposed a model to illustrate the mutational process by which Lynch syndrome progresses to MPC. Results We revealed a novel pathologic mutation on the MSH2 gene (G504 splicing that associates with Lynch syndrome. Systematical comparison of the mutation landscape revealed that multiple cancers in the proband were evolutionarily independent. Integrative analysis showed that truncating mutations of DNA mismatch repair (MMR genes were significantly enriched in the patient. A mutation progress model that included germline mutations of MMR genes, double hits of MMR system, mutations in tissue-specific driver genes, and rapid accumulation of additional passenger mutations was proposed to illustrate how MPC occurs in Lynch syndrome patients. Conclusion Our findings demonstrate that both germline and somatic alterations are driving forces of carcinogenesis, which may resolve the carcinogenic theory of Lynch syndrome.

  11. The Effect of Msh2 Knockdown on Toxicity Induced by tert-Butyl-hydroperoxide, Potassium Bromate, and Hydrogen Peroxide in Base Excision Repair Proficient and Deficient Cells

    Directory of Open Access Journals (Sweden)

    N. Cooley


    Full Text Available The DNA mismatch repair (MMR and base excision repair (BER systems are important determinants of cellular toxicity following exposure to agents that cause oxidative DNA damage. To examine the interactions between these different repair systems, we examined whether toxicity, induced by t-BOOH and KBrO3, differs in BER proficient (Mpg+/+, Nth1+/+ and deficient (Mpg−/−, Nth1−/− mouse embryonic fibroblasts (MEFs following Msh2 knockdown of between 79 and 88% using an shRNA expression vector. Msh2 knockdown in Nth1+/+ cells had no effect on t-BOOH and KBrO3 induced toxicity as assessed by an MTT assay; knockdown in Nth1−/− cells resulted in increased resistance to t-BOOH and KBrO3, a result consistent with Nth1 removing oxidised pyrimidines. Msh2 knockdown in Mpg+/+ cells had no effect on t-BOOH toxicity but increased resistance to KBrO3; in Mpg−/− cells, Msh2 knockdown increased cellular sensitivity to KBrO3 but increased resistance to t-BOOH, suggesting a role for Mpg in removing DNA damage induced by these agents. MSH2 dependent and independent pathways then determine cellular toxicity induced by oxidising agents. A complex interaction between MMR and BER repair systems, that is, exposure dependent, also exists to determine cellular toxicity.

  12. The Effect of Msh2 Knockdown on Toxicity Induced by tert-Butyl-hydroperoxide, Potassium Bromate, and Hydrogen Peroxide in Base Excision Repair Proficient and Deficient Cells (United States)

    Cooley, N.; Elder, R. H.; Povey, A. C.


    The DNA mismatch repair (MMR) and base excision repair (BER) systems are important determinants of cellular toxicity following exposure to agents that cause oxidative DNA damage. To examine the interactions between these different repair systems, we examined whether toxicity, induced by t-BOOH and KBrO3, differs in BER proficient (Mpg +/+, Nth1 +/+) and deficient (Mpg −/−, Nth1 −/−) mouse embryonic fibroblasts (MEFs) following Msh2 knockdown of between 79 and 88% using an shRNA expression vector. Msh2 knockdown in Nth1 +/+ cells had no effect on t-BOOH and KBrO3 induced toxicity as assessed by an MTT assay; knockdown in Nth1 −/− cells resulted in increased resistance to t-BOOH and KBrO3, a result consistent with Nth1 removing oxidised pyrimidines. Msh2 knockdown in Mpg +/+ cells had no effect on t-BOOH toxicity but increased resistance to KBrO3; in Mpg −/− cells, Msh2 knockdown increased cellular sensitivity to KBrO3 but increased resistance to t-BOOH, suggesting a role for Mpg in removing DNA damage induced by these agents. MSH2 dependent and independent pathways then determine cellular toxicity induced by oxidising agents. A complex interaction between MMR and BER repair systems, that is, exposure dependent, also exists to determine cellular toxicity. PMID:23984319

  13. Facilitating NASA Earth Science Data Processing Using Nebula Cloud Computing (United States)

    Chen, A.; Pham, L.; Kempler, S.; Theobald, M.; Esfandiari, A.; Campino, J.; Vollmer, B.; Lynnes, C.


    Cloud Computing technology has been used to offer high-performance and low-cost computing and storage resources for both scientific problems and business services. Several cloud computing services have been implemented in the commercial arena, e.g. Amazon's EC2 & S3, Microsoft's Azure, and Google App Engine. There are also some research and application programs being launched in academia and governments to utilize Cloud Computing. NASA launched the Nebula Cloud Computing platform in 2008, which is an Infrastructure as a Service (IaaS) to deliver on-demand distributed virtual computers. Nebula users can receive required computing resources as a fully outsourced service. NASA Goddard Earth Science Data and Information Service Center (GES DISC) migrated several GES DISC's applications to the Nebula as a proof of concept, including: a) The Simple, Scalable, Script-based Science Processor for Measurements (S4PM) for processing scientific data; b) the Atmospheric Infrared Sounder (AIRS) data process workflow for processing AIRS raw data; and c) the GES-DISC Interactive Online Visualization ANd aNalysis Infrastructure (GIOVANNI) for online access to, analysis, and visualization of Earth science data. This work aims to evaluate the practicability and adaptability of the Nebula. The initial work focused on the AIRS data process workflow to evaluate the Nebula. The AIRS data process workflow consists of a series of algorithms being used to process raw AIRS level 0 data and output AIRS level 2 geophysical retrievals. Migrating the entire workflow to the Nebula platform is challenging, but practicable. After installing several supporting libraries and the processing code itself, the workflow is able to process AIRS data in a similar fashion to its current (non-cloud) configuration. We compared the performance of processing 2 days of AIRS level 0 data through level 2 using a Nebula virtual computer and a local Linux computer. The result shows that Nebula has significantly

  14. Kinetic model of ammonia synthesis in the solar nebula (United States)

    Norris, T. L.


    Kinetic model of ammonia formation by iron catalysis in the primordial solar nebula is developed. The maximum time to reach equilibrium concentration is determined for various temperatures between 1000 and 200 K on the basis of reaction rates derived from industrial data on iron catalysts for ammonia. Application of the method for calculating the equilibrium time to an arbitrary nebula cooling model which maximizes the time available for ammonia synthesis results in an upper limit of 3% of the equilibrium value to the proportion of nitrogen in the form of ammonia at the time of planetary accretion, with ammonia abundance decreasing with distance from the sun. It is concluded that kinetic rather than equilibrium considerations control the abundance of ammonia in the solar nebula, and implications of the dominance of nitrogen for the evolution of the atmospheres of the terrestrial and Jovian planets and the composition of comets are indicated.

  15. The surprising Crab pulsar and its nebula: a review. (United States)

    Bühler, R; Blandford, R


    The Crab nebula and its pulsar (referred to together as 'the Crab') have historically played a central role in astrophysics. True to this legacy, several unique discoveries have been made recently. The Crab was found to emit gamma-ray pulsations up to energies of 400 GeV, beyond what was previously expected from pulsars. Strong gamma-ray flares, of durations of a few days, were discovered from within the nebula, while the source was previously expected to be stable in flux on these time scales. Here we review these intriguing and suggestive developments. In this context we give an overview of the observational properties of the Crab and our current understanding of pulsars and their nebulae.

  16. Lifetime of the solar nebula constrained by meteorite paleomagnetism. (United States)

    Wang, Huapei; Weiss, Benjamin P; Bai, Xue-Ning; Downey, Brynna G; Wang, Jun; Wang, Jiajun; Suavet, Clément; Fu, Roger R; Zucolotto, Maria E


    A key stage in planet formation is the evolution of a gaseous and magnetized solar nebula. However, the lifetime of the nebular magnetic field and nebula are poorly constrained. We present paleomagnetic analyses of volcanic angrites demonstrating that they formed in a near-zero magnetic field (nebula field, and likely the nebular gas, had dispersed by this time. This sets the time scale for formation of the gas giants and planet migration. Furthermore, it supports formation of chondrules after 4563.5 million years ago by non-nebular processes like planetesimal collisions. The core dynamo on the angrite parent body did not initiate until about 4 to 11 million years after solar system formation. Copyright © 2017, American Association for the Advancement of Science.

  17. An Interactive Gallery of Planetary Nebula Spectra (United States)

    Kwitter, K. B.; Henry, R. B. C.


    We have created a website containing high-quality moderate-resolution spectra of 88 planetary nebulae (PNe) from 3600 to 9600 Å, obtained at KPNO and CTIO. Spectra are displayed in a zoomable window, and there are templates available that show wavelength and ion identifications. In addition to the spectra themselves, the website also contains a brief discussion of PNe as astronomical objects and as contributors to our understanding of stellar evolution, and a table with atlas information for each object along with a link to an image. This table can be re-ordered by object name, galactic or equatorial coordinates, distance from the sun, the galactic center, or the galactic plane. We envision that this website, which concentrates a large amount of data in one place, will be of interest to a variety of users. PN researchers might need to check the spectrum of a particular object of interest; the non-specialist astronomer might simply be interested in perusing such a collection of spectra; and finally, teachers of introductory astronomy can use this database to illustrate basic principles of atomic physics and radiation. To encourage such use, we have written two simple exercises at a basic level to introduce beginning astronomy students to the wealth of information that PN spectra contain. We are grateful to Adam Wang of the Williams College OIT and to his summer student teams who worked on various apects of the implementation of this website. This work has been supported by NSF grant AST-9819123 and by Williams College and the University of Oklahoma.

  18. Facilitating NASA Earth Science Data Processing Using Nebula Cloud Computing (United States)

    Pham, Long; Chen, Aijun; Kempler, Steven; Lynnes, Christopher; Theobald, Michael; Asghar, Esfandiari; Campino, Jane; Vollmer, Bruce


    Cloud Computing has been implemented in several commercial arenas. The NASA Nebula Cloud Computing platform is an Infrastructure as a Service (IaaS) built in 2008 at NASA Ames Research Center and 2010 at GSFC. Nebula is an open source Cloud platform intended to: a) Make NASA realize significant cost savings through efficient resource utilization, reduced energy consumption, and reduced labor costs. b) Provide an easier way for NASA scientists and researchers to efficiently explore and share large and complex data sets. c) Allow customers to provision, manage, and decommission computing capabilities on an as-needed bases

  19. Experimental simulations of sulfide formation in the solar nebula. (United States)

    Lauretta, D S; Lodders, K; Fegley, B


    Sulfurization of meteoritic metal in H2S-H2 gas produced three different sulfides: monosulfide solid solution [(Fe,Ni)1-xS], pentlandite [(Fe,Ni)9-xS8], and a phosphorus-rich sulfide. The composition of the remnant metal was unchanged. These results are contrary to theoretical predictions that sulfide formation in the solar nebula produced troilite (FeS) and enriched the remaining metal in nickel. The experimental sulfides are chemically and morphologically similar to sulfide grains in the matrix of the Alais (class CI) carbonaceous chondrite, suggesting that these meteoritic sulfides may be condensates from the solar nebula.

  20. The origin of the most luminous Planetary Nebulae (United States)

    Galera-Rosillo, Rebeca; Corradi, Romano L. M.; Balick, Bruce; Kwitter, Karen; Mampaso, Antonio; García-Rojas, Jorge


    As part of a systematic effort to characterize the properties and progenitors of the most luminous planetary nebulae (PNe), we obtained a sample among the brightest PNe in two stellar systems of different metallicities: LMC (Z/Z⊙~0.5) and M31 (Z/Z⊙~1) by means of a combined effort with the VLT and the 10mGTC. Modelling of these data will allow us to infer the masses of the stellar progenitors, gaining insights into the controversial origin of the universal cutoff of the Planetary Nebulae Luminosity Function (PNLF).

  1. Observing by hand sketching the nebulae in the nineteenth century

    CERN Document Server

    Nasim, Omar W


    Today we are all familiar with the iconic pictures of the nebulae produced by the Hubble Space Telescope's digital cameras. But there was a time, before the successful application of photography to the heavens, in which scientists had to rely on handmade drawings of these mysterious phenomena.           Observing by Hand sheds entirely new light on the ways in which the production and reception of handdrawn images of the nebulae in the nineteenth century contributed to astronomical observation. Omar W. Nasim investigates hundreds of unpublished observing books and paper records from six ninete

  2. Wind Structure and Wind Loading

    DEFF Research Database (Denmark)

    Brorsen, Michael

    The purpose of this note is to provide a short description of wind, i.e. of the flow in the atmosphere of the Earth and the loading caused by wind on structures. The description comprises: causes to the generation of windhe interaction between wind and the surface of the Earthhe stochastic nature...... of windhe interaction between wind and structures, where it is shown that wind loading depends strongly on this interaction...

  3. Stellar winds, fast rotators, and magnetic acceleration (United States)

    Nerney, S.


    The assumption that observed mass outflow from a star is due to a magnetically driven wind implies an upper bound on the surface magnetic field strength from regions where the wind originates. Evidence is reported that corroborates Rosendhal's observation of an abrupt change in the velocity-gradient-luminosity relationship for B8 and later supergiants. The smallest upper bounds correspond to later spectral types, for which radiation would be a relatively inefficient mechanism for driving wind; these winds may in fact be magnetically driven. Be stars are prime candidates for magnetically accelerated winds due to large rotation rates and small mass loss rates. Observed flows can be driven by 0.1-10 gauss surface fields. Intense field in fast rotators can lead to dramatic mass loss rates and large terminal velocities; sigma Ori E may be such an object. Finally, the effect of magnetic acceleration on the dispersal of solar nebula is considered. The increased velocities allow the wind to disperse a mass of 90 times the initial outflowing mass in the wind.

  4. Identification of the amidase BbdA that initiates biodegradation of the groundwater micropollutant 2,6-dichlorobenzamide (BAM) in Aminobacter sp. MSH1

    DEFF Research Database (Denmark)

    T'Syen, Jeroen; Tassoni, Raffaella; Hansen, Lars H.


    2,6-dichlorobenzamide (BAM) is a recalcitrant groundwater micropollutant that poses a major problem for drinking water production in European countries. Aminobacter sp. MSH1 and related strains have the unique ability to mineralize BAM at micropollutant concentrations but no information exists...... on the genetics of BAM biodegradation. An amidase-BbdA-converting BAM to 2,6-dichlorobenzoic acid (DCBA) was purified from Aminobacter sp. MSH1. Heterologous expression of the corresponding bbdA gene and its absence in MSH1 mutants defective in BAM degradation, confirmed its BAM degrading function. BbdA shows low...... amino acid sequence identity with reported amidases and is encoded by an IncP1-β plasmid (pBAM1, 40.6 kb) that lacks several genes for conjugation. BbdA has a remarkably low KM for BAM (0.71 μM) and also shows activity against benzamide and ortho-chlorobenzamide (OBAM). Differential proteomics...

  5. An outflow from the nebula around the LBV candidate S 119


    Weis, Kerstin; Duschl, Wolfgang J.; Bomans, Dominik J.


    We present an analysis of the kinematic and morphological structure of the nebula around the LMC LBV candidate S 119. On HST images, we find a predominantly spherical nebula which, however, seems to be much better confined in its eastern hemisphere than in the western one. The filamentary western part of the nebula is indicative of matter flowing out of the nebula's main body. This outflow is even more evidenced by our long-slit echelle spectra. They show that, while most of the nebula has an...

  6. Rab3a is critical for trapping alpha-MSH granules in the high Ca²⁺-affinity pool by preventing constitutive exocytosis.

    Directory of Open Access Journals (Sweden)

    Simon Sedej

    Full Text Available Rab3a is a small GTPase of the Rab3 subfamily that acts during late stages of Ca²⁺-regulated exocytosis. Previous functional analysis in pituitary melanotrophs described Rab3a as a positive regulator of Ca²⁺-dependent exocytosis. However, the precise role of the Rab3a isoform on the kinetics and intracellular [Ca²⁺] sensitivity of regulated exocytosis, which may affect the availability of two major peptide hormones, α-melanocyte stimulating hormone (α-MSH and β-endorphin in plasma, remain elusive. We employed Rab3a knock-out mice (Rab3a KO to explore the secretory phenotype in melanotrophs from fresh pituitary tissue slices. High resolution capacitance measurements showed that Rab3a KO melanotrophs possessed impaired Ca²⁺-triggered secretory activity as compared to wild-type cells. The hampered secretion was associated with the absence of cAMP-guanine exchange factor II/ Epac2-dependent secretory component. This component has been attributed to high Ca²⁺-sensitive release-ready vesicles as determined by slow photo-release of caged Ca²⁺. Radioimmunoassay revealed that α-MSH, but not β-endorphin, was elevated in the plasma of Rab3a KO mice, indicating increased constitutive exocytosis of α-MSH. Increased constitutive secretion of α-MSH from incubated tissue slices was associated with reduced α-MSH cellular content in Rab3a-deficient pituitary cells. Viral re-expression of the Rab3a protein in vitro rescued the secretory phenotype of melanotrophs from Rab3a KO mice. In conclusion, we suggest that Rab3a deficiency promotes constitutive secretion and underlies selective impairment of Ca²⁺-dependent release of α-MSH.

  7. D-TRP(8-γMSH Prevents the Effects of Endotoxin in Rat Skeletal Muscle Cells through TNFα/NF-KB Signalling Pathway.

    Directory of Open Access Journals (Sweden)

    Ana Belén Gómez-SanMiguel

    Full Text Available Sepsis induces anorexia and muscle wasting secondary to an increase in muscle proteolysis. Melanocyte stimulating hormones (MSH is a family of peptides that have potent anti-inflammatory effects. Melanocortin receptor-3 (MC3-R has been reported as the predominant anti-inflammatory receptor for melanocortins. The aim of this work was to analyse whether activation of MC3-R, by administration of its agonist D-Trp(8-γMSH, is able to modify the response of skeletal muscle to inflammation induced by lipopolysaccharide endotoxin (LPS or TNFα. Adult male rats were injected with 250 μg/kg LPS and/or 500 μg/kg D-Trp(8-γMSH 17:00 h and at 8:00 h the following day, and euthanized 4 hours afterwards. D-Trp(8-γMSH decreased LPS-induced anorexia and prevented the stimulatory effect of LPS on hypothalamic IL-1β, COX-2 and CRH as well as on serum ACTH and corticosterone. Serum IGF-I and its expression in liver and gastrocnemius were decreased in rats injected with LPS, but not in those that also received D-Trp(8-γMSH. However, D-Trp(8-γMSH was unable to modify the effect of LPS on IGFBP-3. In the gastrocnemius D-Trp(8-γMSH blocked LPS-induced decrease in pAkt, pmTOR, MHC I and MCH II, as well as the increase in pNF-κB(p65, FoxO1, FoxO3, LC3b, Bnip-3, Gabarap1, atrogin-1, MuRF1 and in LC3a/b lipidation. In L6 myotube cultures, D-Trp(8-γMSH was able to prevent TNFα-induced increase of NF-κB(p65 phosphorylation and decrease of Akt phosphorylation as well as of IGF-I and MHC I expression. These data suggest that MC3-R activation prevents the effect of endotoxin on skeletal wasting by modifying inflammation, corticosterone and IGF-I responses and also by directly acting on muscle cells through the TNFα/NF-κB(p65 pathway.

  8. Cinnamomum cassia Essential Oil Inhibits α-MSH-Induced Melanin Production and Oxidative Stress in Murine B16 Melanoma Cells

    Directory of Open Access Journals (Sweden)

    Ying Shih


    Full Text Available Essential oils extracted from aromatic plants exhibit important biological activities and have become increasingly important for the development of aromatherapy for complementary and alternative medicine. The essential oil extracted from Cinnamomum cassia Presl (CC-EO has various functional properties; however, little information is available regarding its anti-tyrosinase and anti-melanogenic activities. In this study, 16 compounds in the CC-EO have been identified; the major components of this oil are cis-2-methoxycinnamic acid (43.06% and cinnamaldehyde (42.37%. CC-EO and cinnamaldehyde exhibited anti-tyrosinase activities; however, cis-2-methoxycinnamic acid did not demonstrate tyrosinase inhibitory activity. In murine B16 melanoma cells stimulated with α-melanocyte-stimulating hormone (α-MSH, CC-EO and cinnamaldehyde not only reduced the melanin content and tyrosinase activity of the cells but also down-regulated tyrosinase expression without exhibiting cytotoxicity. Moreover, CC-EO and cinnamaldehyde decreased thiobarbituric acid-reactive substance (TBARS levels and restored glutathione (GSH and catalase activity in the α-MSH-stimulated B16 cells. These results demonstrate that CC-EO and its major component, cinnamaldehyde, possess potent anti-tyrosinase and anti-melanogenic activities that are coupled with antioxidant properties. Therefore, CC-EO may be a good source of skin-whitening agents and may have potential as an antioxidant in the future development of complementary and alternative medicine-based aromatherapy.

  9. Crystal structure of magnesium silicate hydrates (M-S-H): The relation with 2:1 Mg–Si phyllosilicates

    Energy Technology Data Exchange (ETDEWEB)

    Roosz, Cédric, E-mail: [Andra — Scientific Division, 1-7 Rue Jean Monnet, Parc de la Croix Blanche, 92298 Chatenay-Malabry Cedex (France); BRGM, 3 Avenue Claude Guillemin, 45060 Orléans Cedex 2 (France); Grangeon, Sylvain; Blanc, Philippe [BRGM, 3 Avenue Claude Guillemin, 45060 Orléans Cedex 2 (France); Montouillout, Valérie [CNRS, CEMHTI, UPR 3079, 45071 Orléans (France); Lothenbach, Barbara [Empa, Laboratory for Concrete & Construction Chemistry, Überlandstrasse 129, 8600 Dübendorf (Switzerland); Henocq, Pierre; Giffaut, Eric [Andra — Scientific Division, 1-7 Rue Jean Monnet, Parc de la Croix Blanche, 92298 Chatenay-Malabry Cedex (France); Vieillard, Philippe [Université de Poitiers-CNRS, UMR7285 IC2MP, Equipe HydrASA, Poitiers 86022 (France); Gaboreau, Stéphane [BRGM, 3 Avenue Claude Guillemin, 45060 Orléans Cedex 2 (France)


    Two magnesium silicate hydrates (M-S-H) with structural magnesium/silicon ratios of 0.57 ± 0.08 and 1.07 ± 0.13 were synthesized at room temperature, with one year of synthesis duration. Their structure was clarified by considering results from X-ray diffraction, TEM, {sup 29}Si MAS NMR spectroscopy, TGA, and EPMA. A modeling approach appropriate to defective minerals was used because usual XRD refinement techniques cannot be used in the case of turbostratic samples, where coherency between successive layers is lost. M-S-H with Mg/Si ratio of ~ 0.6 appears to be structurally close to nanocrystalline turbostratic 2:1 Mg–Si phyllosilicates. The increase of the Mg/Si ratio from 0.6 to 1.2 occurs by increasing the occurrence of defects in the silicate plan. The layer-to-layer distance evolves from 9.46 Å to 14 Å under air-dried and ethylene glycol conditions, respectively. Crystallites have a mean size of 1.5 nm in the ab plane, and 2.4 nm along c*.

  10. Plumbagin Suppresses α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells by Inhibiting Tyrosinase Activity

    Directory of Open Access Journals (Sweden)

    Taek-In Oh


    Full Text Available Recent studies have shown that plumbagin has anti-inflammatory, anti-allergic, antibacterial, and anti-cancer activities; however, it has not yet been shown whether plumbagin suppresses alpha-melanocyte stimulating hormone (α-MSH-induced melanin synthesis to prevent hyperpigmentation. In this study, we demonstrated that plumbagin significantly suppresses α-MSH-stimulated melanin synthesis in B16F10 mouse melanoma cells. To understand the inhibitory mechanism of plumbagin on melanin synthesis, we performed cellular or cell-free tyrosinase activity assays and analyzed melanogenesis-related gene expression. We demonstrated that plumbagin directly suppresses tyrosinase activity independent of the transcriptional machinery associated with melanogenesis, which includes micropthalmia-associated transcription factor (MITF, tyrosinase (TYR, and tyrosinase-related protein 1 (TYRP1. We also investigated whether plumbagin was toxic to normal human keratinocytes (HaCaT and lens epithelial cells (B3 that may be injured by using skin-care cosmetics. Surprisingly, lower plumbagin concentrations (0.5–1 μM effectively inhibited melanin synthesis and tyrosinase activity but do not cause toxicity in keratinocytes, lens epithelial cells, and B16F10 mouse melanoma cells, suggesting that plumbagin is safe for dermal application. Taken together, these results suggest that the inhibitory effect of plumbagin to pigmentation may make it an acceptable and safe component for use in skin-care cosmetic formulations used for skin whitening.

  11. Cinnamomum cassia essential oil inhibits α-MSH-induced melanin production and oxidative stress in murine B16 melanoma cells. (United States)

    Chou, Su-Tze; Chang, Wen-Lun; Chang, Chen-Tien; Hsu, Shih-Lan; Lin, Yu-Che; Shih, Ying


    Essential oils extracted from aromatic plants exhibit important biological activities and have become increasingly important for the development of aromatherapy for complementary and alternative medicine. The essential oil extracted from Cinnamomum cassia Presl (CC-EO) has various functional properties; however, little information is available regarding its anti-tyrosinase and anti-melanogenic activities. In this study, 16 compounds in the CC-EO have been identified; the major components of this oil are cis-2-methoxycinnamic acid (43.06%) and cinnamaldehyde (42.37%). CC-EO and cinnamaldehyde exhibited anti-tyrosinase activities; however, cis-2-methoxycinnamic acid did not demonstrate tyrosinase inhibitory activity. In murine B16 melanoma cells stimulated with α-melanocyte-stimulating hormone (α-MSH), CC-EO and cinnamaldehyde not only reduced the melanin content and tyrosinase activity of the cells but also down-regulated tyrosinase expression without exhibiting cytotoxicity. Moreover, CC-EO and cinnamaldehyde decreased thiobarbituric acid-reactive substance (TBARS) levels and restored glutathione (GSH) and catalase activity in the α-MSH-stimulated B16 cells. These results demonstrate that CC-EO and its major component, cinnamaldehyde, possess potent anti-tyrosinase and anti-melanogenic activities that are coupled with antioxidant properties. Therefore, CC-EO may be a good source of skin-whitening agents and may have potential as an antioxidant in the future development of complementary and alternative medicine-based aromatherapy.

  12. Cinnamomum cassia Essential Oil Inhibits α-MSH-Induced Melanin Production and Oxidative Stress in Murine B16 Melanoma Cells (United States)

    Chou, Su-Tze; Chang, Wen-Lun; Chang, Chen-Tien; Hsu, Shih-Lan; Lin, Yu-Che; Shih, Ying


    Essential oils extracted from aromatic plants exhibit important biological activities and have become increasingly important for the development of aromatherapy for complementary and alternative medicine. The essential oil extracted from Cinnamomum cassia Presl (CC-EO) has various functional properties; however, little information is available regarding its anti-tyrosinase and anti-melanogenic activities. In this study, 16 compounds in the CC-EO have been identified; the major components of this oil are cis-2-methoxycinnamic acid (43.06%) and cinnamaldehyde (42.37%). CC-EO and cinnamaldehyde exhibited anti-tyrosinase activities; however, cis-2-methoxycinnamic acid did not demonstrate tyrosinase inhibitory activity. In murine B16 melanoma cells stimulated with α-melanocyte-stimulating hormone (α-MSH), CC-EO and cinnamaldehyde not only reduced the melanin content and tyrosinase activity of the cells but also down-regulated tyrosinase expression without exhibiting cytotoxicity. Moreover, CC-EO and cinnamaldehyde decreased thiobarbituric acid-reactive substance (TBARS) levels and restored glutathione (GSH) and catalase activity in the α-MSH-stimulated B16 cells. These results demonstrate that CC-EO and its major component, cinnamaldehyde, possess potent anti-tyrosinase and anti-melanogenic activities that are coupled with antioxidant properties. Therefore, CC-EO may be a good source of skin-whitening agents and may have potential as an antioxidant in the future development of complementary and alternative medicine-based aromatherapy. PMID:24051402

  13. The first frost in the Pipe Nebula (United States)

    Goto, Miwa; Bailey, Jeffrey D.; Hocuk, Seyit; Caselli, Paola; Esplugues, Gisela B.; Cazaux, Stephanie; Spaans, Marco


    Context. Spectroscopic studies of ices in nearby star-forming regions indicate that ice mantles form on dust grains in two distinct steps, starting with polar ice formation (H2O rich) and switching to apolar ice (CO rich). Aims: We test how well the picture applies to more diffuse and quiescent clouds where the formation of the first layers of ice mantles can be witnessed. Methods: Medium-resolution near-infrared spectra are obtained toward background field stars behind the Pipe Nebula. Results: The water ice absorption is positively detected at 3.0 μm in seven lines of sight out of 21 sources for which observed spectra are successfully reduced. The peak optical depth of the water ice is significantly lower than those in Taurus with the same AV. The source with the highest water-ice optical depth shows CO ice absorption at 4.7 μm as well. The fractional abundance of CO ice with respect to water ice is 16-6+7%, and about half as much as the values typically seen in low-mass star-forming regions. Conclusions: A small fractional abundance of CO ice is consistent with some of the existing simulations. Observations of CO2 ice in the early diffuse phase of a cloud play a decisive role in understanding the switching mechanism between polar and apolar ice formation. Based on data collected by SpeX at the Infrared Telescope Facility, which is operated by the University of Hawaii under contract NNH14CK55B with the National Aeronautics and Space Administration.Based also on data obtained at the W.M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California, and the National Aeronautics and Space Administration. The Observatory was made possible by the generous financial support of the W.M. Keck Foundation.The final reduced spectra (FITS format) are available at the CDS via anonymous ftp to ( or via

  14. Expansion patterns and parallaxes for planetary nebulae (United States)

    Schönberner, D.; Balick, B.; Jacob, R.


    Aims: We aim to determine individual distances to a small number of rather round, quite regularly shaped planetary nebulae by combining their angular expansion in the plane of the sky with a spectroscopically measured expansion along the line of sight. Methods: We combined up to three epochs of Hubble Space Telescope imaging data and determined the angular proper motions of rim and shell edges and of other features. These results are combined with measured expansion speeds to determine individual distances by assuming that line of sight and sky-plane expansions are equal. We employed 1D radiation-hydrodynamics simulations of nebular evolution to correct for the difference between the spectroscopically measured expansion velocities of rim and shell and of their respective shock fronts. Results: Rim and shell are two independently expanding entities, driven by different physical mechanisms, although their model-based expansion timescales are quite similar. We derive good individual distances for 15 objects, and the main results are as follows: (i) distances derived from rim and shell agree well; (ii) comparison with the statistical distances in the literature gives reasonable agreement; (iii) our distances disagree with those derived by spectroscopic methods; (iv) central-star "plateau" luminosities range from about 2000 L⊙ to well below 10 000 L⊙, with a mean value at about 5000 L⊙, in excellent agreement with other samples of known distance (Galactic bulge, Magellanic Clouds, and K648 in the globular cluster M 15); (v) the central-star mass range is rather restricted: from about 0.53 to about 0.56 M⊙, with a mean value of 0.55 M⊙. Conclusions: The expansion measurements of nebular rim and shell edges confirm the predictions of radiation-hydrodynamics simulations and offer a reliable method for the evaluation of distances to suited objects. Results of this paper are based on observations made with the NASA/ESA Hubble Space Telescope in Cycle 16 (GO11122

  15. Abundant Solar Nebula Solids in Comets (United States)

    Messenger, S.; Keller, L. P.; Nakamura-Messenger, K.; Nguyen, A. N.; Clemett, S.


    Comets have been proposed to consist of unprocessed interstellar materials together with a variable amount of thermally annealed interstellar grains. Recent studies of cometary solids in the laboratory have shown that comets instead consist of a wide range of materials from across the protoplanetary disk, in addition to a minor complement of interstellar materials. These advances were made possible by the return of direct samples of comet 81P/Wild 2 coma dust by the NASA Stardust mission and recent advances in microscale analytical techniques. Isotopic studies of 'cometary' chondritic porous interplanetary dust particles (CP-IDPs) and comet 81P/Wild 2 Stardust samples show that preserved interstellar materials are more abundant in comets than in any class of meteorite. Identified interstellar materials include sub-micron-sized presolar silicates, oxides, and SiC dust grains and some fraction of the organic material that binds the samples together. Presolar grain abundances reach 1 weight percentage in the most stardust-rich CP-IDPs, 50 times greater than in meteorites. Yet, order of magnitude variations in presolar grain abundances among CP-IDPs suggest cometary solids experienced significant variations in the degree of processing in the solar nebula. Comets contain a surprisingly high abundance of nebular solids formed or altered at high temperatures. Comet 81P/Wild 2 samples include 10-40 micron-sized, refractory Ca- Al-rich inclusion (CAI)-, chondrule-, and ameboid olivine aggregate (AOA)-like materials. The O isotopic compositions of these refractory materials are remarkably similar to their meteoritic counterparts, ranging from 5 percent enrichments in (sup 16) O to near-terrestrial values. Comet 81P/Wild 2 and CP-IDPs also contain abundant Mg-Fe crystalline and amorphous silicates whose O isotopic compositions are also consistent with Solar System origins. Unlike meteorites, that are dominated by locally-produced materials, comets appear to be composed of

  16. The nature of the Napoleon's Hat nebula of SN 1987A (United States)

    Wang, L.; Dyson, J. E.; Kahn, F. D.


    The interstellar and circumstellar environment of SN 1987A is modeled. The geometries of Napoleon's Hat nebula and the dark bay suggest that there is relative motion between the SN progenitor and the surrounding ISM. Most of the dark bay can be identified with the bubble produced by the fast blue supergiant (BSG) wind before the red supergiant (RSG) stage. The relative motion between the star and the ISM is the primary reason for the star being at the edge of this bubble. After the first BSG stage, the star evolves to the RSG stage; the wind velocity is now typically 10 km/s and the mass loss rate is around 10 exp -5 solar mass/yr. The star eventually breaks through the bubble produced in the early BSG stage and starts to interact directly with the ISM outside, thus producing Napoleon's Hat. The success of this model is convincing proof of the BSG-RSG-BSG evolutionary model for the SN 1987A progenitors, and shows that is has moved from the site where it was formed.



    A NASA Hubble Space Telescope view of a turbulent cauldron of starbirth, called N159, taking place 170,000 light-years away in our satellite galaxy, the Large Magellanic Cloud (LMC). Torrential stellar winds from hot newborn massive stars within the nebula sculpt ridges, arcs, and filaments in the vast cloud, which is over 150 light-years across. A rare type of compact ionized 'blob' is resolved for the first time to be a butterfly-shaped or 'Papillon' (French for 'butterfly') nebula, buried in the center of the maelstrom of glowing gases and dark dust. The unprecedented details of the structure of the Papillon, itself less than 2 light-years in size (about 2 arcseconds in the sky), are seen in the inset. A possible explanation of this bipolar shape is the outflow of gas from massive stars (over 10 times the mass of our sun) hidden in the central absorption zone. Such stars are so hot that their radiation pressure halts the infall of gas and directs it away from the stars in two opposite directions. Presumably, a dense equatorial disk formed by matter still trying to fall in onto the stars focuses the outstreaming matter into the bipolar directions. This observation is part of a search for young massive stars in the LMC. Rare are the cases where we can see massive stars so early after their birth. The red in this true-color image is from the emission of hydrogen and the yellow from high excitation ionized oxygen. The picture was taken on September 5, 1998 with the Wide Field Planetary Camera 2. The Hubble observations of the Papillon nebula were conducted by the European astronomers Mohammad Heydari-Malayeri (Paris Observatory, France) and co-investigators Michael Rosa (Space Telescope-European Coordinating Facility, European Southern Observatory, Germany), Vassilis Charmandaris (Paris Observatory), Lise Deharveng (Marseille Observatory, France), and Hans Zinnecker (Astrophysical Institute, Potsdam, Germany). Their work is submitted for publication in the European


    Energy Technology Data Exchange (ETDEWEB)

    Hasegawa, Yasuhiro; Turner, Neal J.; Masiero, Joseph [Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109 (United States); Wakita, Shigeru; Matsumoto, Yuji; Oshino, Shoichi, E-mail: [Center for Computational Astrophysics, National Astronomical Observatory of Japan, Osawa, Mitaka, Tokyo 181-8588 (Japan)


    Chondritic meteorites provide valuable opportunities to investigate the origins of the solar system. We explore impact jetting as a mechanism of chondrule formation and subsequent pebble accretion as a mechanism of accreting chondrules onto parent bodies of chondrites, and investigate how these two processes can account for the currently available meteoritic data. We find that when the solar nebula is ≤5 times more massive than the minimum-mass solar nebula at a ≃ 2–3 au and parent bodies of chondrites are ≤10{sup 24} g (≤500 km in radius) in the solar nebula, impact jetting and subsequent pebble accretion can reproduce a number of properties of the meteoritic data. The properties include the present asteroid belt mass, the formation timescale of chondrules, and the magnetic field strength of the nebula derived from chondrules in Semarkona. Since this scenario requires a first generation of planetesimals that trigger impact jetting and serve as parent bodies to accrete chondrules, the upper limit of parent bodies’ masses leads to the following implications: primordial asteroids that were originally ≥10{sup 24} g in mass were unlikely to contain chondrules, while less massive primordial asteroids likely had a chondrule-rich surface layer. The scenario developed from impact jetting and pebble accretion can therefore provide new insights into the origins of the solar system.

  19. OpenNebula KVM SR-IOV driver

    CSIR Research Space (South Africa)

    Macleod, D


    Full Text Available With the recent release of an OFED which supports SR-IOV on Infiniband HCAs it is now possible to use verbs from inside a VM. This VMM driver supports these Infiniband HCAs, and any other SR-IOV network device, in OpenNebula....

  20. Abundances of planetary nebula M1-42

    NARCIS (Netherlands)

    Pottasch, S. R.; Bernard-Salas, J.; Roellig, T. L.

    The spectra of the planetary nebula M1-42 is reanalysed using spectral measurements made in the mid-infrared with the Spitzer Space Telescope. The aim is to determine the chemical composition of this object. We also make use of ISO, IUE and ground based spectra. Abundances determined from the

  1. Abundances of planetary nebulae NGC 3242 and NGC 6369

    NARCIS (Netherlands)

    Pottasch, S. R.; Bernard-Salas, J.


    The spectra of the planetary nebulae NGC3242 and NGC6369 are reanalysed using spectral measurements made in the mid-infrared with the Spitzer Space Telescope and the Infrared Space Observatory (ISO). The aim is to determine the chemical composition of these objects. We also make use of International

  2. Multibaseline Observations of the Occultation of Crab Nebula by the ...

    Indian Academy of Sciences (India)

    ... Journal of Astrophysics and Astronomy; Volume 21; Issue 3-4. Multibaseline Observations of the Occultation of Crab Nebula by the Solar Corona at Decameter Wavelengths. K. R. Subramanian. Session X – Cycle Variation in the Quiet Corona & Coronal Holes Volume 21 Issue 3-4 September-December 2000 pp 421-422 ...

  3. Modern techniques in galaxy kinematics : Results from planetary nebula spectroscopy

    NARCIS (Netherlands)

    Romanowsky, AJ; Douglas, NG; Kuijken, K; Arnaboldi, M; Gerssen, J; Merrifield, MR; Kwok, S; Dopita, M; Sutherland, R


    We have observed planetary nebulae (PNe) in several early-type galaxies using new techniques on 4- to 8-meter-class telescopes. We obtain the first large data sets (greater than or similar to 100 velocities each) of PN kinematics in galaxies at greater than or similar to 15 Mpc, and present some

  4. Physical conditions in Photo-Dissociation Regions around Planetary Nebulae

    NARCIS (Netherlands)

    Bernard-Salas, J; Tielens, AGGM

    We present observations of the infrared fine-structure lines of [Si II] (34.8 mum), [O I] (63.2 and 145.5 mum) and [C II] (157.7 mum) obtained with the ISO SWS and LWS spectrographs of nine Planetary Nebulae (PNe). These lines originate in the Photo-Dissociation Regions (PDRs) associated with the

  5. Large-Scale Structure of the Carina Nebula. (United States)

    Smith; Egan; Carey; Price; Morse; Price


    Observations obtained with the Midcourse Space Experiment (MSX) satellite reveal for the first time the complex mid-infrared morphology of the entire Carina Nebula (NGC 3372). On the largest size scale of approximately 100 pc, the thermal infrared emission from the giant H ii region delineates one coherent structure: a (somewhat distorted) bipolar nebula with the major axis perpendicular to the Galactic plane. The Carina Nebula is usually described as an evolved H ii region that is no longer actively forming stars, clearing away the last vestiges of its natal molecular cloud. However, the MSX observations presented here reveal numerous embedded infrared sources that are good candidates for sites of current star formation. Several compact infrared sources are located at the heads of dust pillars or in dark globules behind ionization fronts. Because their morphology suggests a strong interaction with the peculiar collection of massive stars in the nebula, we speculate that these new infrared sources may be sites of triggered star formation in NGC 3372.

  6. The ISO-SWS spectrum of planetary nebula NGC 7027

    NARCIS (Netherlands)

    Salas, JB; Pottasch, [No Value; Beintema, DA; Wesselius, PR

    We present the infrared spectrum of the planetary nebula NGC7027 observed with the Short Wavelength Spectrometer (SWS), on board the Infrared Space Observatory (ISO). These data allow us to derive the electron density and, together with the IUE and optical spectra, the electron temperature for

  7. The central star of the Planetary Nebula NGC 6537

    NARCIS (Netherlands)

    Pottasch, [No Value


    The fact that Space Telescope WFPC2 images of the planetary nebula NGC 6537 fail to show the central star is used to derive a limit to its magnitude: it is fainter than a magnitude of 22.4 in the visible. This is used to derive a lower limit to the temperature of the star. The Zanstra temperature is

  8. Hard X-ray Variations in the Crab Nebula (United States)

    Wilson-Hodge, Colleen A.; Cherry, M. L.; Case, G. L.; Baumgartner, W. H.; Beklen, E.; Bhat, P. N.; Briggs, M. S.; Camero-Arranz, A.; Connaughton, V.; Finger, M. H.; hide


    In the first two years of science operations of the Fermi Gamma-ray Burst Monitor (GBM), August 2008 to August 2010, approximately 7% (70 mcrab) decline was discovered in the overall Crab Nebula flux in the 15 - 50 keV band, measured with the Earth occultation technique. This decline was independently confirmed with four other instruments: the RXTE/PCA, Swift/BAT, INTEGRAL/IBIS, and INTEGRAL/SPI. The pulsed flux measured with RXTE/PCA from 1999-2010 was consistent with the pulsar spin-down, indicating that the observed changes were nebular. From 2001 to 2010, the Crab nebula flux measured with RXTE/ PCA was particularly variable, changing by up to approximately 3.5% per year in the 15-50 keV band. These variations were confirmed with INTEGRAL/SPI starting in 2003, Swift/BAT starting in 2005, and Fermi GBM starting in 2008. Before 2001 and since 2010, the Crab nebula flux has appeared more stable, varying by less than 2% per year. I will present updated light curves in multiple energy bands for the Crab Nebula, including recent data from Fermi GBM, Swift/BAT, INTEGRAL and MAXI, and a 16-year long light curve from RXTE/PCA.

  9. Preferrential Concentration of Particles in Protoplanetary Nebula Turbulence (United States)

    Hartlep, Thomas; Cuzzi, Jeffrey N.


    Preferential concentration in turbulence is a process that causes inertial particles to cluster in regions of high strain (in-between high vorticity regions), with specifics depending on their stopping time or Stokes number. This process is thought to be of importance in various problems including cloud droplet formation and aerosol transport in the atmosphere, sprays, and also in the formation of asteroids and comets in protoplanetary nebulae. In protoplanetary nebulae, the initial accretion of primitive bodies from freely-floating particles remains a problematic subject. Traditional growth-by-sticking models encounter a formidable "meter-size barrier" [1] in turbulent nebulae. One scenario that can lead directly from independent nebula particulates to large objects, avoiding the problematic m-km size range, involves formation of dense clumps of aerodynamically selected, typically mm-size particles in protoplanetary turbulence. There is evidence that at least the ordinary chondrite parent bodies were initially composed entirely of a homogeneous mix of such particles generally known as "chondrules" [2]. Thus, while it is arcane, turbulent preferential concentration acting directly on chondrule size particles are worthy of deeper study. Here, we present the statistical determination of particle multiplier distributions from numerical simulations of particle-laden isotopic turbulence, and a cascade model for modeling turbulent concentration at lengthscales and Reynolds numbers not accessible by numerical simulations. We find that the multiplier distributions are scale dependent at the very largest scales but have scale-invariant properties under a particular variable normalization at smaller scales.

  10. Crab Nebula Variations in Hard X-rays (United States)

    Wilson-Hodge, Colleen A.


    The Crab Nebula was surprisingly variable from 2001-2010, with less variability before 2001 and since mid-2010. We presented evidence for spectral softening from RXTE, Swift/BAT, and Fermi GBM during the mid-2008-2010 flux decline. We see no clear connections between the hard X-ray variations and the GeV flares

  11. A 100 kpc nebula associated with the `Teacup' fading quasar (United States)

    Villar-Martín, M.; Cabrera-Lavers, A.; Humphrey, A.; Silva, M.; Ramos Almeida, C.; Piqueras-López, J.; Emonts, B.


    We report the discovery of an ˜100 kpc ionized nebula associated with the radio-quiet type 2 quasar (QSO2) nicknamed the `Teacup' (z = 0.085). The giant nebula is among the largest known around active galaxies at any z. We propose that it is part of the circumgalactic medium (CGM) of the QSO2 host, which has been populated with tidal debris by galactic interactions. This rich gaseous medium has been rendered visible due to the illumination by the powerful active nucleus (AGN). Subsolar abundances (˜0.5 Z⊙) are tentatively favoured by AGN photoionization models. We also report the detection of coronal emission (Fe+6) from the NE bubble, at ˜9 kpc from the AGN. The detection of coronal lines at such large distances from the AGN and the [N II] λ6583/Hα, [S II] λλ6716,6731/Hα, [O I] λ6300/Hα optical emission-line ratios of the giant nebula are consistent with the fading quasar scenario proposed by Gagne et al. The fading rate appears to have been faster in the last ˜46 000 yr. Deep wide field integral field spectroscopy of giant nebulae around powerful AGN such as the `Teacup's' with instruments such as MUSE on VLT opens up a way to detect and study the elusive material from the CGM around massive active galaxies thanks to the illumination by the luminous AGN.


    NARCIS (Netherlands)



    We have studied the IRAS colors of the ring nebula RCW 58 surrounding the Wolf-Rayet star HD 96548 (= WR 40; type WN 8) by analyzing the IRAS survey data with the Groningen Exportable High-Resolution Analysis system (GEISHA) and by using the Chopped Photometric Channel high-resolution imaging at

  13. Local space density and formation rate of planetary nebulae

    NARCIS (Netherlands)

    Pottasch, [No Value

    Individual distances of 50 nearby planetary nebulae are determined using a variety of methods, but excluding statistical methods or distance scales. These distances, together with a discussion of the sample completeness, are used to determine local PN formation rate. Together with the brightness of

  14. The Extended Region Around the Planetary Nebula NGC 3242 (United States)


    This ultraviolet image from NASA's Galaxy Evolution Explorer shows NGC 3242, a planetary nebula frequently referred to as 'Jupiter's Ghost.' The unfortunate name of 'planetary nebula' for this class of celestial object is a historical legacy credited to William Herschel during the 18th century a time when telescopes where small and objects like these, at least the central region, looked very similar to gas-giant planets such as Saturn and Jupiter. In fact, NGC 3242 has no relation to Jupiter or any other planet. Telescopes and their detectors have dramatically improved over the past few centuries. Our understanding of what planetary nebulae truly are has improved accordingly. When stars with a mass similar to our sun approach the end of their lives by exhausting supplies of hydrogen and helium fuel in their cores, they swell up into cool red-giant stars. In a last gasp before death, they expel the layers of gas in their outer atmosphere. This exposes the core of the dying star, a dense hot ball of carbon and oxygen called a white dwarf. The white dwarf is so hot that it shines very brightly in the ultraviolet. The ultraviolet light from the white dwarf, in turn, ionizes the gaseous material expelled by the star causing it to glow. A planetary nebula is really the death of a low-mass star. Although low-mass stars like our sun live for billions of years, planetary nebulae only last for about ten thousand years. As the central white dwarf quickly cools and the ultraviolet light dwindles, the surrounding gas also cools and fades. In this image of NGC 3242 from the Galaxy Evolution Explorer, the extended region around the planetary nebula is shown in dramatic detail. The small circular white and blue area at the center of the image is the well-known portion of the famous planetary nebula. The precise origin and composition of the extended wispy white features is not known for certain. It is most likely material ejected during the star's red-giant phase before the white

  15. Mapping Excitation in the Inner Regions of the Planetary Nebula NGC 5189 Using HST WFC3 Imaging (United States)

    Danehkar, Ashkbiz; Karovska, Margarita; Maksym, W. Peter; Montez, Rodolfo, Jr.


    The planetary nebula (PN) NGC 5189 around a Wolf–Rayet [WO] central star demonstrates one of the most remarkable complex morphologies among PNe with many multiscale structures, showing evidence of multiple outbursts from an asymptotic giant branch (AGB) progenitor. In this study, we use multiwavelength Hubble Space Telescope Wide Field Camera 3 observations to study the morphology of the inner 0.3 pc × 0.2 pc region surrounding the central binary that appears to be a relic of a more recent outburst of the progenitor AGB star. We applied diagnostic diagrams based on emission-line ratios of Hα λ6563, [O III] λ5007, and [S II] λ λ 6716,6731 images to identify the location and morphology of low-ionization structures within the inner nebula. We distinguished two inner, low-ionization envelopes from the ionized gas, within a radius of 55 arcsec (∼0.15 pc) extending from the central star: a large envelope expanding toward the northeast, and its smaller counterpart envelope in the opposite direction toward the southwest of the nebula. These low-ionization envelopes are surrounded by a highly ionized gaseous environment. We believe that these low-ionization expanding envelopes are a result of a powerful outburst from the post-AGB star that created shocked wind regions as they propagate through the previously expelled material along a symmetric axis. Our diagnostic mapping using high-angular resolution line-emission imaging can provide a novel approach to detection of low-ionization regions in other PNe, especially those showing a complex multiscale morphology.

  16. Thyroid cancer in a patient with a germline MSH2 mutation. Case report and review of the Lynch syndrome expanding tumour spectrum

    NARCIS (Netherlands)

    Stulp, Rein P; Herkert, Johanna C; Karrenbeld, Arend; Mol, Bart; Vos, Yvonne J; Sijmons, Rolf H


    Lynch syndrome (HNPCC) is a dominantly inherited disorder characterized by germline defects in DNA mismatch repair (MMR) genes and the development of a variety of cancers, predominantly colorectal and endometrial. We present a 44-year-old woman who was shown to carry the truncating MSH2 gene

  17. MSH2 and MLH1 mutations in sporadic replication error-positive colorectal carcinoma as assessed by two-dimensional DNA electrophoresis

    NARCIS (Netherlands)

    Wu, Y; NystromLahti, M; Osinga, J; Looman, MWG; Peltomaki, P; Aaltonen, LA; delaChapelle, A; Hofstra, RMW; Buys, CHCM

    Replication errors (RER) are frequently seen in both sporadic and hereditary forms of colorectal cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), RER is associated with defects in DNA mismatch repair genes. Two of these genes, MSH2 and MLH1, account for a major share of this cancer

  18. ACTH, alpha-MSH, and control of cortisol release: cloning, sequencing, and functional expression of the melanocortin-2 and melanocortin-5 receptor in Cyprinus carpio

    NARCIS (Netherlands)

    Metz, J.R.; Geven, E.J.W.; Burg, E.H. van den; Flik, G.


    Cortisol release from fish head kidney during the acute phase of the stress response is controlled by the adrenocorticotropic hormone (ACTH) from the pituitary pars distalis (PD). Alpha-melanocyte-stimulating hormone (alpha-MSH) and beta-endorphin, from the pars intermedia (PI), have been implicated

  19. Plasma alpha-MSH and acetylated beta-endorphin levels following stress vary according to CRH sensitivity of the pituitary melanotropes in common carp, Cyprinus carpio.

    NARCIS (Netherlands)

    Burg, E.H. van den; Metz, J.R.; Spanings, F.A.T.; Wendelaar Bonga, S.E.; Flik, G.


    Pituitary melanotropes release alpha-melanocyte-stimulating hormone (alpha-MSH) and acetylated beta-endorphin (NAc beta-end) during stress responses. However, effects of stressors on plasma concentrations of these hormones are highly inconsistent among fish species. Here, we show that also within a

  20. Plasma -MSH and acetylated -endorphin levels following stress vary according to CRH sensitivity of the pituitary melanotropes in common carp, Cyprinus carpio

    NARCIS (Netherlands)

    Burg, E.H. van den; Metz, J.R.; Spanings, F.A.T.; Wendelaar Bonga, S.E.; Flik, G.


    Pituitary melanotropes release alpha-melanocyte-stimulating hormone (alpha-MSH) and acetylated beta-endorphin (NAc beta-end) during stress responses. However, effects of stressors on plasma concentrations of these hormones are highly inconsistent among fish species. Here, we show that also within a

  1. Detection and precise mapping of germline rearrangements in BRCA1, BRCA2, MSH2, and MLH1 using zoom-in array comparative genomic hybridization (aCGH)

    DEFF Research Database (Denmark)

    Staaf, Johan; Törngren, Therese; Rambech, Eva


    deletions or duplications occurring in BRCA1 (n=11), BRCA2 (n=2), MSH2 (n=7), or MLH1 (n=9). Additionally, we demonstrate its applicability for uncovering complex somatic rearrangements, exemplified by zoom-in analysis of the PTEN and CDKN2A loci in breast cancer cells. The sizes of rearrangements ranged...

  2. MLH1 and MSH2 protein expression as a pre-screening marker in hereditary and non-hereditary endometrial hyperplasia and cancer

    NARCIS (Netherlands)

    Berends, MJW; Hollema, H; Wu, Y; van der Sluis, T; Mensink, RGJ; ten Hoor, KA; Sijmons, RH; de Vries, EGE; Pras, E; Mourits, MJE; Hofstra, RMW; Buys, CHCM; Kleibeuker, JH; van der Zee, AGJ


    The predictive value of MLH1 or MSH2 protein expression for the presence of truncating germline mutations was examined in benign and (pre)malignant endometrial samples from 3 patient groups: (1) 10 endometrial cancer patients from hereditary non-polyposis colorectal cancer (HNPCC) families with (n =

  3. Thyroid cancer in a patient with a germline MSH2 mutation. Case report and review of the Lynch syndrome expanding tumour spectrum

    Directory of Open Access Journals (Sweden)

    Stulp Rein P


    Full Text Available Abstract Lynch syndrome (HNPCC is a dominantly inherited disorder characterized by germline defects in DNA mismatch repair (MMR genes and the development of a variety of cancers, predominantly colorectal and endometrial. We present a 44-year-old woman who was shown to carry the truncating MSH2 gene mutation that had previously been identified in her family. Recently, she had been diagnosed with an undifferentiated carcinoma of the thyroid and an adenoma of her coecum. Although the thyroid carcinoma was not MSI-high (1 out of 5 microsatellites instable, it did show complete loss of immunohistochemical expression for the MSH2 protein, suggesting that this tumour was not coincidental. Although the risks for some tumour types, including breast cancer, soft tissue sarcoma and prostate cancer, are not significantly increased in Lynch syndrome, MMR deficiency in the presence of a corresponding germline defect has been demonstrated in incidental cases of a growing range of tumour types, which is reviewed in this paper. Interestingly, the MSH2-associated tumour spectrum appears to be wider than that of MLH1 and generally the risk for most extra-colonic cancers appears to be higher for MSH2 than for MLH1 mutation carriers. Together with a previously reported case, our findings show that anaplastic thyroid carcinoma can develop in the setting of Lynch syndrome. Uncommon Lynch syndrome-associated tumour types might be useful in the genetic analysis of a Lynch syndrome suspected family if samples from typical Lynch syndrome tumours are unavailable.

  4. Development of mismatch amplification mutation assays for the differentiation of MS1 vaccine strain from wild-type Mycoplasma synoviae and MS-H vaccine strains (United States)

    Kreizinger, Zsuzsa; Sulyok, Kinga Mária; Grózner, Dénes; Bekő, Katinka; Dán, Ádám; Szabó, Zoltán


    Mycoplasma synoviae is an economically significant pathogen in the poultry industry, inducing respiratory disease and infectious synovitis in chickens and turkeys, and eggshell apex abnormality in chickens. Eradication, medication and vaccination are the options for controlling M. synoviae infection. Currently there are two commercial, live, attenuated vaccines available against M. synoviae: the temperature sensitive MS-H vaccine strain and the NAD independent MS1 vaccine strain. Differentiation of vaccine strains from field isolates is essential during vaccination and eradication programs. The present study provides melt-curve and agarose gel based mismatch amplification mutation assays (MAMA) to discriminate the MS1 vaccine strain from the MS-H vaccine strain and wild-type M. synoviae isolates. The assays are based on the A/C single nucleotide polymorphism at nt11 of a HIT family protein coding gene. The melt- and agarose-MAMAs reliably distinguish the MS1 vaccine strain genotype from the MS-H vaccine strain and wild-type M. synoviae isolate genotype from 102 template number/DNA sample. No cross-reactions with other avian Mycoplasma species were observed. The assays can be performed directly on clinical samples and they can be run simultaneously with the previously described MAMAs designed for the discrimination of the MS-H vaccine strain. The developed assays are applicable in laboratories with limited facilities and promote the rapid, simple and cost effective differentiation of the MS1 vaccine strain. PMID:28419134

  5. Mutation in flrA and mshA Genes of Vibrio cholerae Inversely Involved in vps-Independent Biofilm Driving Bacterium Toward Nutrients in Lake Water. (United States)

    Sinha-Ray, Shrestha; Ali, Afsar


    Many bacterial pathogens promote biofilms that confer resistance against stressful survival conditions. Likewise Vibrio cholerae O1, the causative agent of cholera, and ubiquitous in aquatic environments, produces vps-dependent biofilm conferring resistance to environmental stressors and predators. Here we show that a 49-bp deletion mutation in the flrA gene of V. cholerae N16961S strain resulted in promotion of vps-independent biofilm in filter sterilized lake water (FSLW), but not in nutrient-rich L-broth. Complementation of flrA mutant with the wild-type flrA gene inhibited vps-independent biofilm formation. Our data demonstrate that mutation in the flrA gene positively contributed to vps-independent biofilm production in FSLW. Furthermore, inactivation of mshA gene, encoding the main pilin of mannose sensitive hemagglutinin (MSHA pilus) in the background of a ΔflrA mutant, inhibited vps-independent biofilm formation. Complementation of ΔflrAΔmshA double mutant with wild-type mshA gene restored biofilm formation, suggesting that mshA mutation inhibited ΔflrA-driven biofilm. Taken together, our data suggest that V. cholerae flrA and mshA act inversely in promoting vps-independent biofilm formation in FSLW. Using a standard chemotactic assay, we demonstrated that vps-independent biofilm of V. cholerae, in contrast to vps-dependent biofilm, promoted bacterial movement toward chitin and phosphate in FSLW. A ΔflrAΔmshA double mutant inhibited the bacterium from moving toward nutrients; this phenomenon was reversed with reverted mutants (complemented with wild-type mshA gene). Movement to nutrients was blocked by mutation in a key chemotaxis gene, cheY-3, although, cheY-3 had no effect on vps-independent biofilm. We propose that in fresh water reservoirs, V. cholerae, on repression of flagella, enhances vps-independent biofilm that aids the bacterium in acquiring nutrients, including chitin and phosphate; by doing so, the microorganism enhances its ability to

  6. Measurements of Magnetic Fields in the Solar Nebula (United States)

    Fu, R. R.; Andrade Lima, E.; Weiss, B. P.


    magnetization blocked up to 200 mT in addition to the MC component. The HC component is not present in surrounding bulk samples, suggesting that it pre-dates the accretion of the meteorite and likely records nebular magnetic fields. Using the anhysteretic remanent magnetization (ARM) normalization method, the HC component corresponds to a paleointensity of 6 μT. Analysis of additional dusty olivine-bearing chondrules, including thermal demagnetization, is ongoing. The existence of pre-accretional remanence in chondrules implies that magnetic fields during chondrule formation were stable on the timescale of chondrule cooling. The relatively low paleointensity of 6 μT, if confirmed by further experiments, is consistent with magnetic fields in the hypothesized "dead zone" at the mid-plane of the solar nebula where the MRI is expected to be weak. Chondrule records of dead zone magnetic fields imply that chondrule formation occurred near the disk mid-plane at between approximately 0.5 and 10 AU. Furthermore, this paleointensity may not be consistent with chondrule formation mechanisms that generate strong local magnetic fields, such as the nebular lightning and the X-winds models.

  7. Comprehensive population-wide analysis of Lynch syndrome in Iceland reveals founder mutations in MSH6 and PMS2

    DEFF Research Database (Denmark)

    Haraldsdottir, Sigurdis; Rafnar, Thorunn; Frankel, Wendy L


    Lynch syndrome, caused by germline mutations in the mismatch repair genes, is associated with increased cancer risk. Here using a large whole-genome sequencing data bank, cancer registry and colorectal tumour bank we determine the prevalence of Lynch syndrome, associated cancer risks...... and pathogenicity of several variants in the Icelandic population. We use colorectal cancer samples from 1,182 patients diagnosed between 2000-2009. One-hundred and thirty-two (11.2%) tumours are mismatch repair deficient per immunohistochemistry. Twenty-one (1.8%) have Lynch syndrome while 106 (9.0%) have somatic...... hypermethylation or mutations in the mismatch repair genes. The population prevalence of Lynch syndrome is 0.442%. We discover a translocation disrupting MLH1 and three mutations in MSH6 and PMS2 that increase endometrial, colorectal, brain and ovarian cancer risk. We find thirteen mismatch repair variants...

  8. A new planetary nebula in the outer reaches of the Galaxy

    DEFF Research Database (Denmark)

    Viironen, K.; Mampaso, A.; L. M. Corradi, R.


    A proper determination of the abundance gradient in the Milky Way requires the observation of objects at large galactiocentric distances. With this aim, we are exploring the planetary nebula population towards the Galactic Anticentre. In this article, the discovery and physico-chemical study...... of a new planetary nebula towards the Anticentre direction, IPHASX J052531.19+281945.1 (PNG 178.1-04.0), is presented. The planetary nebula was discovered from the IPHAS survey. Long-slit follow-up spectroscopy was carried out to confirm its planetary nebula nature and to calculate its physical...... and chemical characteristics. The newly discovered planetary nebula turned out to be located at a very large galactocentric distance (D_GC=20.8+-3.8 kpc), larger than any previously known planetary nebula with measured abundances. Its relatively high oxygen abundance (12+log(O/H) = 8.36+-0.03) supports...

  9. Wind turbines

    Energy Technology Data Exchange (ETDEWEB)

    Yeoman, J.C. Jr.


    This evaluation of wind turbines is part of a series of Technology Evaluations of possible components and subsystems of community energy systems. Wind turbines, ranging in size from 200 W to 10 MW, are discussed as candidates for prime movers in community systems. Estimates of performance characteristics and cost as a function of rated capacity and rated wind speed are presented. Data concerning material requirements, environmental effects, and operating procedures also are given and are represented empirically to aid computer simulation.

  10. Wind Energy

    Energy Technology Data Exchange (ETDEWEB)

    Ganley, Jason; Zhang, Jie; Hodge, Bri-Mathias


    Wind energy is a variable and uncertain renewable resource that has long been used to produce mechanical work, and has developed into a large producer of global electricity needs. As renewable sources of energy and feedstocks become more important globally to produce sustainable products, many different processes have started adopting wind power as an energy source. Many times this is through a conversion to hydrogen through electrolysis that allows for a more continuous process input. Other important pathways include methanol and ammonia. As the demand for sustainable products and production pathways increases, and wind power capital costs decrease, the role of wind power in chemical and energy production seems poised to increase significantly.

  11. Al-Sufi's Investigation of Stars, Star Clusters and Nebulae (United States)

    Hafez, Ihsan; Stephenson, F. R.; Orchiston, W.


    The distinguished Arabic astronomer, Al-Sufi (AD 903-986) is justly famous for his Book of the Fixed Stars, an outstanding Medieval treatise on astronomy that was assembled in 964. Developed from Ptolemy's Algamest, but based upon al-Sufi's own stellar observations, the Book of the Fixed Stars has been copied down through the ages, and currently 35 copies are known to exist in various archival repositories around the world. Among other things, this major work contains 55 astronomical tables, plus star charts for 48 constellations. For the first time a long-overdue English translation of this important early work is in active preparation. In this paper we provide biographical material about Al-Sufi and the contents of his Book of the Fixed Stars, before examining his novel stellar magnitude system, and his listing of star clusters and nebulae (including the first-ever mention of the Great Nebula in Andromeda).

  12. Hydrodynamic Instability and Enhanced Transport in Protoplanetary Nebulae (United States)

    Richard, Denis T.


    The nature of turbulence (and the enhanced transport it provides) is a key element to comprehend the dynamics, physics and chemistry of the protoplanetary nebulae and consequently the planet formation process. Early accretion disk models postulated the turbulent transport through the well-known "alpha-viscosity" model, introduced by Shakura and Sunyaev in 1973. Since then, the nature of the turbulence in disks has been a subject of investigation. In 1991, the rediscovery by Balbus and Hawley of Chandrasekhar's linear instability in magnetized disks was a breakthrough in the discipline. Unfortunately, the mechanisms leading to turbulence in non-magnetized disks, such as protoplanetary nebulae, remain poorly understood. We will present results from laboratory experiments along with analytical arguments showing that, despite skepticism in the Astrophysical community, differential rotation may indeed be sufficient to trigger and sustain turbulence. We will also propose an alternative viscosity prescription derived from both experiments and analysis.


    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Y.; Chau, W.; Hsia, C.-H.; Kwok, S. [Department of Physics, University of Hong Kong, Pokfulam Road (Hong Kong); Fang, X.; Liu, X.-W. [Department of Astronomy, Peking University, Beijing 100871 (China); Koning, N., E-mail: [Department of Physics and Astronomy, University of Calgary, Calgary T2N 1N4 (Canada)


    NGC 2392 is a young double-shell planetary nebula (PN). Its intrinsic structure and shaping mechanism are still not fully understood. In this paper we present new spectroscopic observations of NGC 2392. The slits were placed at two different locations to obtain the spectra of the inner and outer regions. Several [Fe III] lines are clearly detected in the inner region. We infer that NGC 2392 might have an intrinsic structure similar to the bipolar nebula Mz 3, which also exhibits a number of [Fe III] lines arising from the central regions. In this scenario, the inner and outer regions of NGC 2392 correspond to the inner lobes and the outer outflows of Mz 3, respectively. We construct a three-dimensional morpho-kinematic model to examine our hypothesis. We also compare the physical conditions and chemical composition of the inner and outer regions, and discuss the implications on the formation of this type of PN.

  14. Spectrophotometry of Bowen resonance fluorescence lines in three planetary nebulae (United States)

    O'Dell, C. R.; Miller, Christopher O.


    The results are presented of a uniquely complete, carefully reduced set of observations of the O III Bowen fluorescence lines in the planetary nebulae NGC 6210, NGC 7027, and NGC 7662. A detailed comparison with the predictions of radiative excitation verify that some secondary lines are enhanced by selective population by the charge exchange mechanism involving O IV. Charge exchange is most important in NGC 6210, which is of significantly lower ionization than the other nebulae. In addition to the principal Bowen lines arising from Ly-alpha pumping of the O III O1 line, lines arising from pumping of the O3 line are also observed. Comparison of lines produced by O1 and O3 with the theoretical predictions of Neufeld indicate poor agreement; comparison with the theoretical predictions of Harrington show agreement with NGC 7027 and NGC 7662.

  15. Catalysis by Dust Grains in the Solar Nebula (United States)

    Kress, Monika E.; Tielens, Alexander G. G. M.


    In order to determine whether grain-catalyzed reactions played an important role in the chemistry of the solar nebula, we have applied our time-dependent model of methane formation via Fischer-Tropsch catalysis to pressures from 10(exp -5) to 1 bar and temperatures from 450 to 650 K. Under these physical conditions, the reaction 3H2 + CO yields CH4 + H2O is readily catalyzed by an iron or nickel surface, whereas the same reaction is kinetically inhibited in the gas phase. Our model results indicate that under certain nebular conditions, conversion of CO to methane could be extremely efficient in the presence of iron-nickel dust grains over timescales very short compared to the lifetime of the solar nebula.

  16. Planetesimal Sizes and Mars Formation in the Magnetized Solar Nebula (United States)

    Hasegawa, Yasuhiro; Morishima, Ryuji


    The Hf-W chronology inferred from Martian meteorites suggests that Mars should be a stranded planetary embryo formed within a very short (about 2 Myr) accretion timescale. Previous studies show that such rapid growth can be realized when small (nebula, focusing on MHD turbulence. We demonstrate what mass of planetesimals can contribute to Mars formation and what value of the nebular mass is needed to satisfy the rapid accretion timescale. We therefore derive a more realistic condition of the solar nebula under which Mars formation took place. While this study is based on the standard picture of runaway and oligarchic growth, we also discuss other formation mechanisms in order to compare how our results would be consistent with the properties of the solar system. These mechanisms are a hypothesis that Mars formed from a narrow ring of planetesimals, and the pebble accretion scenario.

  17. Planet destruction and the shaping of planetary nebulae (United States)

    Boyle, L. A.; Redman, M. P.


    The shaping of PNe as a result of an interaction with a planet is a hypothesis that has been suggested for nearly two decades. However, exploring the idea observationally is challenging due to the lack of capabilities needed to detect any evidence of such a scenario. Nonetheless, we propose that the hypothesis can be indirectly tested via a combination of exoplanet formation and evolution theories, the star and planet formation histories of the galaxy and the tidal evolution of star-planet systems. We present a calculation of the fraction of planetary nebulae in the galaxy today which have undergone an interaction with a planet, concluding that a significant number of visible planetary nebulae may have been shaped by a planet.

  18. Million-degree plasma pervading the extended Orion Nebula. (United States)

    Güdel, Manuel; Briggs, Kevin R; Montmerle, Thierry; Audard, Marc; Rebull, Luisa; Skinner, Stephen L


    Most stars form as members of large associations within dense, very cold (10 to 100 kelvin) molecular clouds. The nearby giant molecular cloud in Orion hosts several thousand stars of ages less than a few million years, many of which are located in or around the famous Orion Nebula, a prominent gas structure illuminated and ionized by a small group of massive stars (the Trapezium). We present x-ray observations obtained with the X-ray Multi-Mirror satellite XMM-Newton, revealing that a hot plasma with a temperature of 1.7 to 2.1 million kelvin pervades the southwest extension of the nebula. The plasma flows into the adjacent interstellar medium. This x-ray outflow phenomenon must be widespread throughout our Galaxy.

  19. Planetary Nebula Candidates Uncovered with the HASH Research Platform (United States)

    Fragkou, Vasiliki; Bojičić, Ivan; Frew, David; Parker, Quentin


    A detailed examination of new high quality radio catalogues (e.g. Cornish) in combination with available mid-infrared (MIR) satellite imagery (e.g. Glimpse) has allowed us to find 70 new planetary nebula (PN) candidates based on existing knowledge of their typical colors and fluxes. To further examine the nature of these sources, multiple diagnostic tools have been applied to these candidates based on published data and on available imagery in the HASH (Hong Kong/ AAO/ Strasbourg Hα planetary nebula) research platform. Some candidates have previously-missed optical counterparts allowing for spectroscopic follow-up. Indeed, the single object spectroscopically observed so far has turned out to be a bona fide PN.

  20. Gamma-ray flares from the Crab Nebula. (United States)

    Abdo, A A; Ackermann, M; Ajello, M; Allafort, A; Baldini, L; Ballet, J; Barbiellini, G; Bastieri, D; Bechtol, K; Bellazzini, R; Berenji, B; Blandford, R D; Bloom, E D; Bonamente, E; Borgland, A W; Bouvier, A; Brandt, T J; Bregeon, J; Brez, A; Brigida, M; Bruel, P; Buehler, R; Buson, S; Caliandro, G A; Cameron, R A; Cannon, A; Caraveo, P A; Casandjian, J M; Çelik, Ö; Charles, E; Chekhtman, A; Cheung, C C; Chiang, J; Ciprini, S; Claus, R; Cohen-Tanugi, J; Costamante, L; Cutini, S; D'Ammando, F; Dermer, C D; de Angelis, A; de Luca, A; de Palma, F; Digel, S W; do Couto e Silva, E; Drell, P S; Drlica-Wagner, A; Dubois, R; Dumora, D; Favuzzi, C; Fegan, S J; Ferrara, E C; Focke, W B; Fortin, P; Frailis, M; Fukazawa, Y; Funk, S; Fusco, P; Gargano, F; Gasparrini, D; Gehrels, N; Germani, S; Giglietto, N; Giordano, F; Giroletti, M; Glanzman, T; Godfrey, G; Grenier, I A; Grondin, M-H; Grove, J E; Guiriec, S; Hadasch, D; Hanabata, Y; Harding, A K; Hayashi, K; Hayashida, M; Hays, E; Horan, D; Itoh, R; Jóhannesson, G; Johnson, A S; Johnson, T J; Khangulyan, D; Kamae, T; Katagiri, H; Kataoka, J; Kerr, M; Knödlseder, J; Kuss, M; Lande, J; Latronico, L; Lee, S-H; Lemoine-Goumard, M; Longo, F; Loparco, F; Lubrano, P; Madejski, G M; Makeev, A; Marelli, M; Mazziotta, M N; McEnery, J E; Michelson, P F; Mitthumsiri, W; Mizuno, T; Moiseev, A A; Monte, C; Monzani, M E; Morselli, A; Moskalenko, I V; Murgia, S; Nakamori, T; Naumann-Godo, M; Nolan, P L; Norris, J P; Nuss, E; Ohsugi, T; Okumura, A; Omodei, N; Ormes, J F; Ozaki, M; Paneque, D; Parent, D; Pelassa, V; Pepe, M; Pesce-Rollins, M; Pierbattista, M; Piron, F; Porter, T A; Rainò, S; Rando, R; Ray, P S; Razzano, M; Reimer, A; Reimer, O; Reposeur, T; Ritz, S; Romani, R W; Sadrozinski, H F-W; Sanchez, D; Saz Parkinson, P M; Scargle, J D; Schalk, T L; Sgrò, C; Siskind, E J; Smith, P D; Spandre, G; Spinelli, P; Strickman, M S; Suson, D J; Takahashi, H; Takahashi, T; Tanaka, T; Thayer, J B; Thompson, D J; Tibaldo, L; Torres, D F; Tosti, G; Tramacere, A; Troja, E; Uchiyama, Y; Vandenbroucke, J; Vasileiou, V; Vianello, G; Vitale, V; Wang, P; Wood, K S; Yang, Z; Ziegler, M


    A young and energetic pulsar powers the well-known Crab Nebula. Here, we describe two separate gamma-ray (photon energy greater than 100 mega-electron volts) flares from this source detected by the Large Area Telescope on board the Fermi Gamma-ray Space Telescope. The first flare occurred in February 2009 and lasted approximately 16 days. The second flare was detected in September 2010 and lasted approximately 4 days. During these outbursts, the gamma-ray flux from the nebula increased by factors of four and six, respectively. The brevity of the flares implies that the gamma rays were emitted via synchrotron radiation from peta-electron-volt (10(15) electron volts) electrons in a region smaller than 1.4 × 10(-2) parsecs. These are the highest-energy particles that can be associated with a discrete astronomical source, and they pose challenges to particle acceleration theory.

  1. Kinematics of the ring-like nebula SuWt 2

    DEFF Research Database (Denmark)

    Jones, D.; Lloyd, M.; Mitchell, D. L.


    We present the first detailed spatio-kinematical analysis and modelling of the Southern planetary nebula SuWt 2. This object presents a problem for current theories of planetary nebula formation and evolution, as it is not known to contain a central post-main sequence star. Deep narrowband [NII]6......, we are unable to rule out the presence of a third component to the system, which would have been the nebula progenitor....

  2. YSOVAR: Six Pre-main-sequence Eclipsing Binaries in the Orion Nebula Cluster (United States)


    reserved. Printed in the U.S.A. YSOVAR: SIX PRE-MAIN-SEQUENCE ECLIPSING BINARIES IN THE ORION NEBULA CLUSTER M. Morales-Calderón1,2, J. R. Stauffer1, K. G...multi-color light curves for∼2400 candidate Orion Nebula Cluster (ONC) members from our Warm Spitzer Exploration Science Program YSOVAR, we have...readable tables 1. INTRODUCTION The Orion Nebula Cluster (ONC) contains several thousand members, and since it is nearby, it provides an excellent em


    Energy Technology Data Exchange (ETDEWEB)

    Koning, N. [Department of Physics and Astronomy, University of Calgary, Calgary, Alberta T2N 1N4 (Canada); Kwok, Sun [Department of Physics, University of Hong Kong, Pokfulam, Hong Kong (Hong Kong); Steffen, W., E-mail:, E-mail:, E-mail: [Intituto de Astronomia Universidad Nacional Autonoma de Mexico, C.P. 22860, Ensenada (Mexico)


    A model for post asymptotic giant branch bipolar reflection nebulae has been constructed based on a pair of evacuated cavities in a spherical dust envelope. Many of the observed features of bipolar nebulae, including filled bipolar lobes, an equatorial torus, searchlight beams, and a bright central light source, can be reproduced. The effects on orientation and dust densities are studied and comparisons with some observed examples are offered. We suggest that many observed properties of bipolar nebulae are the result of optical effects and any physical modeling of these nebulae has to take these factors into consideration.



    In the most active starburst region in the local universe lies a cluster of brilliant, massive stars, known to astronomers as Hodge 301. Hodge 301, seen in the lower right hand corner of this image, lives inside the Tarantula Nebula in our galactic neighbor, the Large Magellanic Cloud. This star cluster is not the brightest, or youngest, or most populous star cluster in the Tarantula Nebula -- that honor goes to the spectacular R136. In fact, Hodge 301 is almost 10 times older than the young cluster R136. But age has its advantages; many of the stars in Hodge 301 are so old that they have exploded as supernovae. These exploded stars are blasting material out into the surrounding region at speeds of almost 200 miles per second. This high speed ejecta are plowing into the surrounding Tarantula Nebula, shocking and compressing the gas into a multitude of sheets and filaments, seen in the upper left portion of the picture. Note for your calendar; Hodge 301 contains three red supergiants - stars that are close to the end of their evolution and are about to go supernova, exploding and sending more shocks into the Tarantula. Also present near the center of the image are small, dense gas globules and dust columns where new stars are being formed today, as part of the overall ongoing star formation throughout the Tarantula region. Credit: Hubble Heritage Team (AURA/STScI/NASA)

  5. Isotopic homogeneity of iron in the early solar nebula. (United States)

    Zhu, X K; Guo, Y; O'Nions, R K; Young, E D; Ash, R D


    The chemical and isotopic homogeneity of the early solar nebula, and the processes producing fractionation during its evolution, are central issues of cosmochemistry. Studies of the relative abundance variations of three or more isotopes of an element can in principle determine if the initial reservoir of material was a homogeneous mixture or if it contained several distinct sources of precursor material. For example, widespread anomalies observed in the oxygen isotopes of meteorites have been interpreted as resulting from the mixing of a solid phase that was enriched in 16O with a gas phase in which 16O was depleted, or as an isotopic 'memory' of Galactic evolution. In either case, these anomalies are regarded as strong evidence that the early solar nebula was not initially homogeneous. Here we present measurements of the relative abundances of three iron isotopes in meteoritic and terrestrial samples. We show that significant variations of iron isotopes exist in both terrestrial and extraterrestrial materials. But when plotted in a three-isotope diagram, all of the data for these Solar System materials fall on a single mass-fractionation line, showing that homogenization of iron isotopes occurred in the solar nebula before both planetesimal accretion and chondrule formation.

  6. The Formation of Graphite Whiskers in the Primitive Solar Nebula (United States)

    Nuth, Joseph A., III; Kimura, Yuki; Lucas, Christopher; Ferguson, Frank; Johnson, Natasha M.


    It has been suggested that carbonaceous grains are efficiently destroyed in the interstellar medium and must either reform in situ at very low pressures and temperatures or in an alternative environment more conducive to grain growth. Graphite whiskers have been discovered associated with high-temperature phases in meteorites such as calcium aluminum inclusions and chondrules, and it has been suggested that the expulsion of such material from protostellar nebulae could significantly affect the optical properties of the average interstellar grain population. We have experimentally studied the potential for Fischer-Tropsch and Haber-Bosch type reactions to produce organic materials in protostellar systems from the abundant H2, CO, and N2 reacting on the surfaces of available silicate grains. When graphite grains are repeatedly exposed to H2, CO, and N2 at 875 K abundant graphite whiskers are observed to form on or from the surfaces of the graphite grains. In a dense, turbulent nebula, such extended whiskers are very likely to be broken off, and fragments could be ejected either in polar jets or by photon pressure after transport to the outer reaches of the nebula.

  7. The Formation of Graphite Whiskers in the Primitive Solar Nebula (United States)

    Nuth, Joseph A., III; Kimura, Yuki; Lucas, Christopher; Ferguson, Frank; Johnson, Natasha M.


    It has been suggested that carbonaceous grains are efficiently destroyed in the interstellar medium and must either reform in situ at very low pressures and temperatures or in an alternative environment more conducive to grain growth. Graphite whiskers have been discovered associated with high-temperature phases in meteorites such as calcium aluminum inclusions and chondrules, and it has been suggested that the expulsion of such material from proto stellar nebulae could significantly affect the optical properties of the average interstellar grain population. We have experimentally studied the potential for Fischer-Tropsch and Haber-Bosch type reactions to produce organic materials in protostellar systems from the abundant H2, CO, and N2 reacting on the surfaces of available silicate grains. When graphite grains are repeatedly exposed to H2, CO, and N2 at 875 K abundant graphite whiskers are observed to form on or from the surfaces of the graphite grains. In a dense, turbulent nebula, such extended whiskers are very likely to be broken off, and fragments could be ejected either in polar jets or by photon pressure after transport to the outer reaches of the nebula.

  8. The Herschel Planetary Nebula Survey (HerPlaNS): A Comprehensive Dusty Photoionization Model of NGC6781. (United States)

    Otsuka, Masaaki; Ueta, Toshiya; van Hoof, Peter A M; Sahai, Raghvendra; Aleman, Isabel; Zijlstra, Albert A; Chu, You-Hua; Villaver, Eva; Leal-Ferreira, Marcelo L; Kastner, Joel; Szczerba, Ryszard; Exter, Katrina M


    We perform a comprehensive analysis of the planetary nebula (PN) NGC 6781 to investigate the physical conditions of each of its ionized, atomic, and molecular gas and dust components and the object's evolution, based on panchromatic observational data ranging from UV to radio. Empirical nebular elemental abundances, compared with theoretical predictions via nucleosynthesis models of asymptotic giant branch (AGB) stars, indicate that the progenitor is a solar-metallicity, 2.25-3.0 M⊙ initial-mass star. We derive the best-fit distance of 0.46 kpc by fitting the stellar luminosity (as a function of the distance and effective temperature of the central star) with the adopted post-AGB evolutionary tracks. Our excitation energy diagram analysis indicates high-excitation temperatures in the photodissociation region (PDR) beyond the ionized part of the nebula, suggesting extra heating by shock interactions between the slow AGB wind and the fast PN wind. Through iterative fitting using the Cloudy code with empirically derived constraints, we find the best-fit dusty photoionization model of the object that would inclusively reproduce all of the adopted panchromatic observational data. The estimated total gas mass (0.41 M⊙) corresponds to the mass ejected during the last AGB thermal pulse event predicted for a 2.5 M⊙ initial-mass star. A significant fraction of the total mass (about 70%) is found to exist in the PDR, demonstrating the critical importance of the PDR in PNe that are generally recognized as the hallmark of ionized/H(+) regions.

  9. Planetary Nebulae in the MW, LMC, SMC: Results from FUSE and HST data (United States)

    Herald, J. E.; Bianchi, L.


    We use FUSE and HST data to study Planetary Nebulae (PN) systems in the Milky Way, LMC and SMC. Theoretically, metallicity affects several aspects of the post-AGB evolution, including mass-loss and the yield of processed material, which are important factors in the chemical evolution of galaxies. Therefore, it is very important to study PNe in different environments. In Bianchi's FUSE programs, we observed CSPN in the Milky Way (Cycle 1), LMC (Cycle 2) and SMC (Cyde 3), representing a range of metallicities from solar to 1/10th solar. The far-UV range reveals the spectrum of the central star (CSPN), uniquely enabling a direct estimate of the ionizing source parameters. Combined with archive HST data, these spectra provide a measurement of T(sub eff), log g, L(sub bol), abundances, wind velocity and mass-loss rate for these post-AGB stars. Additionally, these spectra provide a measurement of the circumstellar H2 and HI, which added to the mass of the central star and of the ionized shell allows us to test theoretical initial-final mass relations, and to put together a complete picture of the star's evolution.

  10. A magnetic torsional wave near the Galactic Centre traced by a 'double helix' nebula. (United States)

    Morris, Mark; Uchida, Keven; Do, Tuan


    The magnetic field in the central few hundred parsecs of the Milky Way has a dipolar geometry and is substantially stronger than elsewhere in the Galaxy, with estimates ranging up to a milligauss (refs 1-6). Characterization of the magnetic field at the Galactic Centre is important because it can affect the orbits of molecular clouds by exerting a drag on them, inhibit star formation, and could guide a wind of hot gas or cosmic rays away from the central region. Here we report observations of an infrared nebula having the morphology of an intertwined double helix about 100 parsecs from the Galaxy's dynamical centre, with its axis oriented perpendicular to the Galactic plane. The observed segment is about 25 parsecs in length, and contains about 1.25 full turns of each of the two continuous, helically wound strands. We interpret this feature as a torsional Alfvén wave propagating vertically away from the Galactic disk, driven by rotation of the magnetized circumnuclear gas disk. The direct connection between the circumnuclear disk and the double helix is ambiguous, but the images show a possible meandering channel that warrants further investigation.

  11. An XMM-Newton Study of 9SGR and the Lagoon Nebula (United States)

    Rauw, G.; Blomme, R.; Waldron, W. L.; Naze, Y.; Harries, T. J.; Chapman, J. M.; Corcoran, M. F.; Detal, A.; Gosset, E.


    We report preliminary results of an XMM-Newton observation of the 04 V star 9 Sgr (= HD 164794). 9 Sgr is one of a few single OB stars that display a non-thermal radio emission attributed to synchrotron emission by relativistic electrons. Inverse Compton scattering of photospheric UV photons by these relativistic electrons is a priori expected to generate a non-thermal power-law tail in the X-ray spectrum. Our EPIC and RGS spectra of 9 Sgr suggest a more complex situation than expected from this 'simple' theoretical picture. Furthermore, soft-band EPIC images of the region around 9 Sgr reveal a number of point sources inside the Lagoon Nebula (M8). Most of these sources have optical counterparts inside the very young open cluster NGC 6530 and several X-ray sources are associated with low and intermediate mass pre-main sequence stars. Finally, we also detect (probably) diffuse X-ray emission from the Hourglass Region that might reveal a hot bubble blown by the stellar wind of Herschel 36, the ionizing star of the HG region.

  12. Localised, high-speed flows within the hydrogen-deficient planetary nebula Abell 78 (United States)

    Meaburn, J.; Lopez, J. A.; Bryce, M.; Redman, M. P.


    The remarkable velocity structure of the different components of the hydrogen-deficient planetary nebula Abell 78 have been revealed by obtaining spatially resolved profiles of the [O i] 6300 Angstromsii\\ line with the Manchester echelle spectrometer combined with the 2.1 m San Pedro Martir telescope. The outer, diffuse, hydrogen-rich, 124'' diameter shell is expanding radially at 40 km s^{-1}$. The irregular, knotty, hydrogen-deficient 89arcsec \\times 52arcsec \\ inner shell has a similar overall expansion velocity but more complex kinematical structure. Similar to A 30, velocity `spikes' are found in the position-velocity arrays of [O {sc i}] 6300 Angstromsii\\ profiles from the inner shell. These extend to a further -140 km s^{-1} than the radial velocity of the approaching side of this inner shell. The [O {sc i}] 6300 Angstromsii\\ bright, hydrogen-deficient core knots are distributed throughout an elongated disk expanding at \\approx 25 km s^{-1}. There is kinematical evidence that `polar bullets' are being ejected perpendicularly to this central feature at 380 km s^{-1}. Altogether `velocity spikes' and other high-speed knots in the pv arrays of [O {sc i}] 6300 Angstromsii\\ profiles are found over a 455 km s^{-1}$ range of radial velocities. Many of the kinematical phenomena are considered to be a consequence of the mass-loaded, shocked, wind from the central star.

  13. Bow shock nebulae of hot massive stars in a magnetized medium (United States)

    Meyer, D. M.-A.; Mignone, A.; Kuiper, R.; Raga, A. C.; Kley, W.


    A significant fraction of OB-type, main-sequence massive stars are classified as runaway and move supersonically through the interstellar medium (ISM). Their strong stellar winds interact with their surroundings, where the typical strength of the local ISM magnetic field is about 3.5-7 μG, which can result in the formation of bow shock nebulae. We investigate the effects of such magnetic fields, aligned with the motion of the flow, on the formation and emission properties of these circumstellar structures. Our axisymmetric, magneto-hydrodynamical simulations with optically thin radiative cooling, heating and anisotropic thermal conduction show that the presence of the background ISM magnetic field affects the projected optical emission of our bow shocks at Hα and [O III] λ 5007 which become fainter by about 1-2 orders of magnitude, respectively. Radiative transfer calculations against dust opacity indicate that the magnetic field slightly diminishes their projected infrared emission and that our bow shocks emit brightly at 60 μm. This may explain why the bow shocks generated by ionizing runaway massive stars are often difficult to identify. Finally, we discuss our results in the context of the bow shock of ζ Ophiuchi and we support the interpretation of its imperfect morphology as an evidence of the presence of an ISM magnetic field not aligned with the motion of its driving star.

  14. A detailed study of the structure of the nested planetary nebula, Hb 12, the Matryoshka nebula

    Energy Technology Data Exchange (ETDEWEB)

    Clark, D. M.; López, J. A. [Instituto de Astronomía, Universidad Nacional Autónoma de México, Campus Ensenada, Ensenada, Baja California, 22860 (Mexico); Edwards, M. L. [LBT Observatory, University of Arizona, 933 North Cherry Avenue, Tucson, AZ 85721 (United States); Winge, C., E-mail:, E-mail:, E-mail:, E-mail: [Gemini Observatory, Southern Operations Center, c/o AURA Inc., Casilla 603, La Serena (Chile)


    We present near-IR, integral field spectroscopic observations of the planetary nebula (PN) Hb 12 using Near-infrared Integral Field Spectrograph (NIFS) on Gemini-North. Combining NIFS with the adaptive optics system Altair, we provide a detailed study of the core and inner structure of this PN. We focus the analysis in the prominent emission lines [Fe II] (1.6436 μm), He I (2.0585 μm), H{sub 2} (2.1214 μm), and Br{sub γ} (2.16553 μm). We find that the [Fe II] emission traces a tilted system of bipolar lobes, with the northern lobe being redshifted and the southern lobe blueshifted. The [Fe II] emission is very faint at the core and only present close to the systemic velocity. There is no H{sub 2} emission in the core, whereas the core is prominent in the He I and Br{sub γ} recombination lines. The H{sub 2} emission is concentrated in equatorial arcs of emission surrounding the core and expanding at ∼30 km s{sup –1}. These arcs are compared with Hubble Space Telescope images and shown to represent nested loops belonging to the inner sections of a much larger bipolar structure that replicates the inner one. The He I and Br{sub γ} emission from the core clearly show a cylindrical central cavity that seems to represent the inner walls of an equatorial density enhancement or torus. The torus is 0.''2 wide (≡200 AU radius at a distance of 2000 pc) and expanding at ≤30 km s{sup –1}. The eastern wall of the inner torus is consistently more intense than the western wall, which could indicate the presence of an off-center star, such as is observed in the similar hourglass PN, MyCn 18. A bipolar outflow is also detected in Br{sub γ} emerging within 0.''1 from the core at ∼ ± 40 km s{sup –1}.

  15. Models of the Mass-ejection Histories of Pre-planetary Nebulae. II. The Formation of Minkowski’s Butterfly and its Proboscis in M2–9 (United States)

    Balick, Bruce; Frank, Adam; Liu, Baowei; Corradi, Romano


    M2–9, or the “Minkowski’s Butterfly,” is one of the most iconic outflow sources from an evolved star. In this paper we present a hydrodynamic model of M2–9 in which the nebula is formed and shaped by a steady, low-density (“light”), mildly collimated “spray” of gas injected at 200 km s‑1 that interacts with a far denser, intrinsically simple pre-existing AGB wind that has slowly formed all of the complex features within M2–9’s lobes (including the knot pairs N3/S3 and N4/S4 at their respective leading edges, and the radial gradient of Doppler shifts within 20″ of the nucleus). We emphasize that the knot pairs are not ejected from the star but formed in situ. In addition, the observed radial speed of the knots is only indirectly related to the speed of the gas injected by the star. The model allows us to probe the early history of the wind geometry and lobe formation. We also formulate a new estimate of the nebular distance D = 1.3 kpc. The physical mechanism that accounts for the linear radial speed gradient in M2–9 applies generally to many other pre-planetary nebulae whose hollow lobes exhibit similar gradients along their edges.

  16. Broadband x-ray imaging and spectroscopy of the crab nebula and pulsar with NuSTAR

    DEFF Research Database (Denmark)

    Madsen, Kristin K.; Reynolds, Stephen; Harrison, Fiona


    We present broadband (3-78 keV) NuSTAR X-ray imaging and spectroscopy of the Crab nebula and pulsar. We show that while the phase-averaged and spatially integrated nebula + pulsar spectrum is a power law in this energy band, spatially resolved spectroscopy of the nebula finds a break at ~9 ke...


    NARCIS (Netherlands)



    Radio continuum emission has been detected from a planetary nebula in the Large Magellanic Cloud: this is the first radio continuum detection for any planetary nebula outside our galaxy. The radio flux density is a factor of two lower than predicted from the HP flux. This could be due either to a

  18. The Spitzer IRS infrared spectrum and abundances of the planetary nebula IC 2448

    NARCIS (Netherlands)

    Guiles, S.; Bernard-Salas, J.; Pottasch, S. R.; Roellig, T. L.


    We present the mid-infrared spectrum of the planetary nebula IC 2448. In order to determine the chemical composition of the nebula, we use the infrared line fluxes from the Spitzer spectrum along with optical line fluxes from the literature and ultraviolet line fluxes from archival IUE spectra. We

  19. Dark-Matter Content of Early-Type Galaxies with Planetary Nebulae

    NARCIS (Netherlands)

    Napolitano, N.R.; Romanowsky, A.J.; Coccato, L; Capaccioli, M.; Douglas, N.G.; Noordermeer, E.; Merrifield, M.R.; Kuijken, K.; Arnaboldi, M.; Gerhard, O.; Freeman, K.C.; De Lorenzi, F.; Das, P.


    Abstract. We examine the dark matter properties of nearby early-type galaxies using plane- tary nebulae (PNe) as mass probes. We have designed a specialised instrument, the Planetary Nebula Spectrograph (PN.S) operating at the William Herschel telescope, with the purpose of measuring PN velocities

  20. The light element abundance distribution in NGC 5128 from planetary nebulae

    NARCIS (Netherlands)

    Walsh, J. R.; Jacoby, G. H.; Peletier, R. F.; Walton, N. A.

    Context. Planetary nebulae in the nearest large elliptical galaxy provide light element abundances difficult or impossible to measure by other means in a stellar system very different from the galaxies in the Local Group. Aims. The light element abundance pattern from many planetary nebulae (PNe) at

  1. Proof of polar ejection fom the close-binary core of the planetary nebula Abell 63

    DEFF Research Database (Denmark)

    Mitchell, Deborah L.; Pollacco, Don; O'Brien, T. J.


    We present the first detailed kinematical analysis of the planetary nebula Abell 63, which is known to contain the eclipsing close-binary nucleus UU Sge. Abell 63 provides an important test case in investigating the role of close-binary central stars on the evolution of planetary nebulae. Longsli...

  2. Nebula--a web-server for advanced ChIP-seq data analysis. (United States)

    Boeva, Valentina; Lermine, Alban; Barette, Camille; Guillouf, Christel; Barillot, Emmanuel


    ChIP-seq consists of chromatin immunoprecipitation and deep sequencing of the extracted DNA fragments. It is the technique of choice for accurate characterization of the binding sites of transcription factors and other DNA-associated proteins. We present a web service, Nebula, which allows inexperienced users to perform a complete bioinformatics analysis of ChIP-seq data. Nebula was designed for both bioinformaticians and biologists. It is based on the Galaxy open source framework. Galaxy already includes a large number of functionalities for mapping reads and peak calling. We added the following to Galaxy: (i) peak calling with FindPeaks and a module for immunoprecipitation quality control, (ii) de novo motif discovery with ChIPMunk, (iii) calculation of the density and the cumulative distribution of peak locations relative to gene transcription start sites, (iv) annotation of peaks with genomic features and (v) annotation of genes with peak information. Nebula generates the graphs and the enrichment statistics at each step of the process. During Steps 3-5, Nebula optionally repeats the analysis on a control dataset and compares these results with those from the main dataset. Nebula can also incorporate gene expression (or gene modulation) data during these steps. In summary, Nebula is an innovative web service that provides an advanced ChIP-seq analysis pipeline providing ready-to-publish results. Nebula is available at Supplementary data are available at Bioinformatics online.

  3. Using slitless spectroscopy to study the kinematics of the planetary nebula population in M94

    NARCIS (Netherlands)

    Douglas, NG; Gerssen, J; Kuijken, K; Merrifield, MR


    The planetary nebula populations of relatively nearby galaxies can be easily observed and provide both a distance estimate and a tool with which dynamical information can be obtained. Usually the requisite radial velocities are obtained by multi-object spectroscopy once the planetary nebulae have

  4. Dissipation of the striped pulsar wind (United States)

    Cerutti, B.; Philippov, A. A.


    Context. Rapidly rotating neutron stars blow a relativistic, magnetized wind mainly composed of electron-positron pairs. The free expansion of the wind terminates far from the neutron star where a weakly magnetized pulsar wind nebula forms, implying efficient magnetic dissipation somewhere upstream. Aims: The wind current sheet that separates the two magnetic polarities is usually considered as the most natural place for magnetic dissipation via relativistic reconnection, but its efficiency remains an open question. Here, the goal of this work is to revisit this issue in light of the most recent progress in the understanding of reconnection and pulsar electrodynamics. Methods: We perform large two-dimensional particle-in-cell simulations of the oblique rotator to capture the multi-scale evolution of the wind. Our simulations are limited to the equatorial plane. Results: We find that the current sheet breaks up into a dynamical chain of magnetic islands separated by secondary thin current sheets. The sheet thickness increases linearly with radius while the Poynting flux decreases monotonically as reconnection proceeds. The radius of complete annihilation of the stripes is given by the plasma multiplicity parameter at the light cylinder. Current starvation within the sheets does not occur before complete dissipation as long as there is enough charges where the sheets form. Particles are efficiently heated up to a characteristic energy set by the magnetization parameter at the light cylinder. Energetic pulsed synchrotron emission peaks close to the light cylinder, and presents sub-pulse variability associated with the formation of plasmoids in the sheet. Conclusions: This study suggests that the striped component of the wind dissipates far before reaching the termination shock in isolated pulsars, even in very-high-multiplicity systems such as the Crab pulsar. Pulsars in binary systems may provide the best environments to study magnetic dissipation in the wind.

  5. Bacterial ClpB heat-shock protein, an antigen-mimetic of the anorexigenic peptide ?-MSH, at the origin of eating disorders


    Tennoune, N; Chan, P; Breton, J; Legrand, R.; Chabane, Y N; Akkermann, K; J?rv, A; Ouelaa, W; Takagi, K.; Ghouzali, I; Francois, M; de Lucas, N; Bole-Feysot, C; Pestel-Caron, M.; do Rego, J-C


    The molecular mechanisms at the origin of eating disorders (EDs), including anorexia nervosa (AN), bulimia and binge-eating disorder (BED), are currently unknown. Previous data indicated that immunoglobulins (Igs) or autoantibodies (auto-Abs) reactive with ?-melanocyte-stimulating hormone (?-MSH) are involved in regulation of feeding and emotion; however, the origin of such auto-Abs is unknown. Here, using proteomics, we identified ClpB heat-shock disaggregation chaperone protein of commensal...

  6. Using 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in flow through biofilters--initial adhesion and BAM degradation potentials. (United States)

    Albers, Christian Nyrop; Jacobsen, Ole Stig; Aamand, Jens


    Micropollutants in groundwater are given significant attention by water companies and authorities due to an increasing awareness that they might be present even above the legal threshold values. As part of our investigations of the possibility to remove the common groundwater pollutant 2,6-dichlorobenzamide (BAM) by introducing the efficient BAM degrader Aminobacter sp. MSH1 into biologically active sand filters, we investigated if the strain adheres to filters containing various filter materials and if the initial adherence and subsequent degradation of BAM could be optimized. We found that most of the inoculated MSH1 cells adhered fast and that parameters like pH and ionic strength had only a minor influence on the adhesion despite huge influence on cell surface hydrophobicity. At the given growth protocol, the MSH1 strain apparently developed a subpopulation that had lost its ability to adhere to the filter materials, which was supported by attempted reinoculation of non-adhered cells. Analysis by quantitative PCR showed that most cells adhered in the top of the filters and that some of these were lost from the filters during initial operation, while insignificant losses occurred after 1 day of operation. The inoculated filters were found to degrade 2.7 μg/L BAM to below 0.1 μg/L at a 1.1-h residence time with insignificant formation of known degradation products. In conclusion, most filter materials and water types should be feasible for inoculation with the MSH1 strain, while more research into degradation at low concentrations and temperatures is needed before this technology is ready for use at actual waterworks.

  7. Protozoa graze on the 2,6-dichlorobenzamide (BAM)-degrading bacterium Aminobacter sp. MSH1 introduced into waterworks sand filters. (United States)

    Ellegaard-Jensen, Lea; Albers, Christian N; Aamand, Jens


    Groundwater contamination by pesticide residues often leads to the closure of drinking water wells, making the development of new techniques to remediate drinking water resources of considerable interest. Pesticide-degrading bacteria were recently added to a waterworks sand filter in an attempt to remediate pesticide-polluted drinking water. The density of the introduced bacteria, however, decreased rapidly, which was partly attributed to predation by protozoa in the sand filter. This study investigated the effects of indigenous sand filter protozoa on the population density and degradation efficiency of degrader bacteria introduced into sand from a waterworks sand filter. The 2,6-dichlorobenzamide (BAM)-degrading bacterium Aminobacter sp. MSH1 was used as a model organism. The introduction of MSH1 at high cell densities was followed by a >1000-fold increase in the protozoan population size and at the same time a 29 % reduction in Aminobacter cell numbers. The protozoan population in the systems that had MSH1 added at a lower density only increased 50-fold, and a decrease in Aminobacter numbers was not detectable. Furthermore, a reduction in the number of Aminobacter and in BAM degradation efficiency was seen in flow-through sand filter columns inoculated with MSH1 and fed BAM-contaminated water, when comparing sand columns containing the indigenous microbial filter community, i.e. containing protozoa, to columns with sterilised sand. These results suggest that degrader bacteria introduced into waterworks sand filters are adversely affected by grazing from the indigenous protozoa, reducing the size of the degrader population and the sand filter degradation efficiency.

  8. World Wind (United States)

    National Aeronautics and Space Administration — World Wind allows any user to zoom from satellite altitude into any place on Earth, leveraging high resolution LandSat imagery and SRTM elevation data to experience...

  9. The complete Einstein Observatory X-ray survey of the Orion Nebula region. (United States)

    Gagne, Marc; Caillault, Jean-Pierre


    We have analyzed archival Einstein Observatory images of a roughly 4.5 square degree region centered on the Orion Nebula. In all, 245 distinct X-ray sources have been detected in six High Resolution Imager (HRI) and 17 Imaging Proportional Counter (IPC) observations. An optical database of over 2700 stars has been assembled to search for candidate counterparts to the X-ray sources. Roughly half the X-ray sources are identified with a single Orion Nebula cluster member. The 10 main-sequence O6-B5 cluster stars detected in Orion have X-ray activity levels comparable to field O and B stars. X-ray emission has also been detected in the direction of four main-sequence late-B and early-A type stars. Since the mechanisms producing X-rays in late-type coronae and early-type winds cannot operate in the late-B and early-A type atmospheres, we argue that the observed X-rays, with L(sub X) approximately = 3 x 10(exp 30) ergs/s, are probably produced in the coronae of unseen late-type binary companions. Over 100 X-ray sources have been associated with late-type pre-main sequence stars. The upper envelope of X-ray activity rises sharply from mid-F to late-G, with L(sub x)/L(sub bol) in the range 10(exp -4) to 2 x 10(exp -3) for stars later than approximately G7. We have looked for variability of the late-type cluster members on timescales of a day to a year and find that 1/4 of the stars show significantly variable X-ray emission. A handful of the late-type stars have published rotational periods and spectroscopic rotational velocities; however, we see no correlation between X-ray activity and rotation. Thus, for this sample of pre-main-sequence stars, the large dispersion in X-ray activity does not appear to be caused by the dispersion in rotation, in contrast with results obtained for low-mass main-sequence stars in the Pleiades and pre-main-sequence stars in Taurus-Auriga.

  10. The region of the supernova remnant MSH 15-52 revisited - A new thermal H II region, H II G 320.5-1.4 (United States)

    Lortet, M.-C.; Georgelin, Y. P.; Georgelin, Y. M.


    The authors revisited the stellar and nebular content in the direction of MSH 15-52. This search was initiated by the discovery of a new Hα thermal region H II G 320.5-1.4 with velocity VLSR = -43 km s-1, extending over an area similar to MSH 15-52, and clearly distinct from the foreground H II region BBW 28802. From a rediscussion of the reddening and distances of hot stars with available spectra in the direction l = 320°, it is found that they constitute a single stellar association (Cir OB1) at a distance about 4 kpc, probably not much more extended than 80×80 pc. This association contains the cluster Pis 20, four WR stars and a number of stars with ages in the range 4 - 10×106yr. It is the excitation source of H II G 320.5-1.4. In such an association, bubbles may have formed previously to the explosion of supernovae; also, several supernovae may have exploded recently. Thus it is not unlikely that MSH 15-52 originated from the same SN explosion as PSR 1509-58 and expanded freely into a bubble; on the other hand, it would not be surprising that two different supernovae exploded close in time and space.

  11. 2,6-Dichlorobenzamide (BAM) herbicide mineralisation by Aminobacter sp. MSH1 during starvation depends on a subpopulation of intact cells maintaining vital membrane functions

    Energy Technology Data Exchange (ETDEWEB)

    Sjoholm, Ole R.; Nybroe, Ole [Department of Agriculture and Ecology, Section of Genetics and Microbiology, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C (Denmark); Aamand, Jens [Department of Agriculture and Ecology, Section of Genetics and Microbiology, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C (Denmark); Department of Geochemistry, Geological Survey of Denmark and Greenland, Oster Voldgade 10, 1350 Copenhagen K (Denmark); Sorensen, Jan, E-mail: jan@life.ku.d [Department of Agriculture and Ecology, Section of Genetics and Microbiology, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C (Denmark)


    Mineralisation capability was studied in the 2,6-dichlorobenzamide (BAM)-degrading Aminobacter sp. MSH1 under growth-arrested conditions. Cells were starved in mineral salts (MS) solution or groundwater before {sup 14}C-labelled BAM (0.1 mM) was added. Cell physiology was monitored with a panel of vitality stains combined with flow cytometry to differentiate intact, depolarised and dead cells. Cells starved for up to 3 weeks in MS solution showed immediate growth-linked mineralisation after BAM amendment while a lag-phase was seen after 8 weeks of starvation. In contrast, cells amended with BAM in natural groundwater showed BAM mineralisation but no growth. The cell-specific mineralisation rate was always comparable (10{sup -16} mol C intact cell{sup -1} day{sup -1}) independent of media, growth, or starvation period after BAM amendment; lower rates were only observed as BAM concentration decreased. MSH1 seems useful for bioremediation and should be optimised to maintain an intact cell subpopulation as this seems to be the key parameter for successful mineralisation. - The intact cell population of Aminobacter MSH1 mineralises BAM at a constant rate independent of growth or extended starvation in mineral solution and natural groundwater.

  12. Rapid, Simple and Cost-Effective Molecular Method to Differentiate the Temperature Sensitive (ts+) MS-H Vaccine Strain and Wild-Type Mycoplasma synoviae Isolates (United States)

    Kreizinger, Zsuzsa; Sulyok, Kinga Mária; Pásztor, Alexandra; Erdélyi, Károly; Felde, Orsolya; Povazsán, János; Kőrösi, László; Gyuranecz, Miklós


    Mycoplasma synoviae infection in chickens and turkeys can cause respiratory disease, infectious synovitis and eggshell apex abnormality; thus it is an economically important pathogen. Control of M. synoviae infection comprises eradication, medication or vaccination. The differentiation of the temperature sensitive (ts+) MS-H vaccine strain from field isolates is crucial during vaccination programs. Melt-curve and agarose gel based mismatch amplification mutation assays (MAMA) are provided in the present study to distinguish between the ts+ MS-H vaccine strain, its non-temperature sensitive re-isolates and wild-type M. synoviae isolates based on the single nucleotide polymorphisms at nt367 and nt629 of the obg gene. The two melt-MAMAs and the two agarose-MAMAs clearly distinguish the ts+ MS-H vaccine strain genotype from its non-temperature sensitive re-isolate genotype and wild-type M. synoviae isolate genotype, and no cross-reactions with other Mycoplasma species infecting birds occur. The sensitivity of the melt-MAMAs and agarose-MAMAs was 103 and 104 copy numbers, respectively. The assays can be performed directly on clinical samples and they can be run simultaneously at the same annealing temperature. The assays can be performed in laboratories with limited facilities, using basic real-time PCR machine or conventional thermocycler coupled with agarose gel electrophoresis. The advantages of the described assays compared with previously used methods are simplicity, sufficient sensitivity, time and cost effectiveness and specificity. PMID:26207635

  13. 78 FR 29364 - Exelon Corporation, Exelon Wind 1, LLC, Exelon Wind 2, LLC, Exelon Wind 3, LLC, Exelon Wind 4... (United States)


    ...-005, QF07-257-004] Exelon Corporation, Exelon Wind 1, LLC, Exelon Wind 2, LLC, Exelon Wind 3, LLC, Exelon Wind 4, LLC, Exelon Wind 5, LLC, Exelon Wind 6, LLC, Exelon Wind 7, LLC, Exelon Wind 8, LLC, Exelon Wind 9, LLC, Exelon Wind 10, LLC, Exelon Wind 11, LLC, High Plains Wind Power, LLC v. Xcel Energy...

  14. A 15N-poor isotopic composition for the solar system as shown by Genesis solar wind samples. (United States)

    Marty, B; Chaussidon, M; Wiens, R C; Jurewicz, A J G; Burnett, D S


    The Genesis mission sampled solar wind ions to document the elemental and isotopic compositions of the Sun and, by inference, of the protosolar nebula. Nitrogen was a key target element because the extent and origin of its isotopic variations in solar system materials remain unknown. Isotopic analysis of a Genesis Solar Wind Concentrator target material shows that implanted solar wind nitrogen has a (15)N/(14)N ratio of 2.18 ± 0.02 × 10(-3) (that is, ≈40% poorer in (15)N relative to terrestrial atmosphere). The (15)N/(14)N ratio of the protosolar nebula was 2.27 ± 0.03 × 10(-3), which is the lowest (15)N/(14)N ratio known for solar system objects. This result demonstrates the extreme nitrogen isotopic heterogeneity of the nascent solar system and accounts for the (15)N-depleted components observed in solar system reservoirs.

  15. Calm winds (United States)

    Ólafsson, Haraldur


    Knowledge of calm winds is of societal importance in connection with distribution pollution from natural sources (dust, volcanic gases and ash) as well as antropogenic sources. Time series from a multitude of automatic weather stations in Iceland have been explored and the climatology of calmness is established. This climatology underlines the importancec of not only abscence of large scale winds, but more importantly, the presence of surface inversions. Calmness is most frequent in summer, with secondary maxima in autumn and winter. The autumn calmness coincides with a period when frequency of synoptic scale cyclones does not increase, while the frequency of surface inversions increases rapidly. There is a very strong diurnal cycle in frequency of calm winds in the summer. The data indiates strongly that the nocturnal calmness is a result of a surface inversion, not the abscence of sea breeze. The frequency of calm winds is not only low at the coast, but also in the mountains, in spite of higher surface roughness away from the sea. The frequency of calm winds is much greater inside valleys and fjords than anywhere else. There are indications that open water in fjords has limited effect on the frequecy of calm winds along the fjord.

  16. Infrared Observations of Cassiopeia A and the Crab Nebula (United States)

    Lawrence, S.; Bohigas, J.


    We have used the Camaleon infrared spectrograph/camera on the 2.1-m telescope of the Observatorio Astronomico Nacional of Mexico to image the Cassiopiea A and Crab Nebula supernova remnants in various bands between 1.0 and 2.5 mu m. Camaleon uses a 256x256 NICMOS array detector. The low resolution imaging mode used for these observations has a field of view of 3.63 arcminutes and an image scale of 0.85 arcseconds per pixel. Mosaics of Cas A were observed in J, K', and deeply in H. As expected, extended emission was detected in the H band, arising from the [Fe II] 1.644 mu m line in the ejecta. Abundance anisotropies have been inferred in the fast moving knots on the basis of the relative strengths of their oxygen and sulfur lines. Due to the heavy extinction towards Cas A, the infrared iron line may be best to investigate the distribution of iron-peak elements. We will present calibrated maps of the [Fe II] emission, and compare the intensity distribution with those of the optical oxygen and sulfur lines. We will also discuss the relative strengths of the [Fe II] emission between the fast moving knots and the quasi-stationary flocculi. Mosaics of the Crab Nebula were taken in J, H, K', and deeply in a narrow filter centered on the H2 2.122 mu m line. Our goal is to map the H2 emission from the dense cores of the ejecta filaments (as detected by Graham et al. 1990). We will also present maps of the infrared spectral index variations of the synchrotron nebula.

  17. Discovering Planetary Nebula Geometries: Explorations with a Hierarchy of Models (United States)

    Huyser, Karen A.; Knuth, Kevin H.; Fischer, Bernd; Schumann, Johann; Granquist-Fraser, Domhnull; Hajian, Arsen R.


    Astronomical objects known as planetary nebulae (PNe) consist of a shell of gas expelled by an aging medium-sized star as it makes its transition from a red giant to a white dwarf. In many cases this gas shell can be approximately described as a prolate ellipsoid. Knowledge of the physics of ionization processes in this gaseous shell enables us to construct a model in three dimensions (3D) called the Ionization-Bounded Prolate Ellipsoidal Shell model (IBPES model). Using this model we can generate synthetic nebular images, which can be used in conjunction with Hubble Space Telescope (HST) images of actual PNe to perform Bayesian model estimation. Since the IBPES model is characterized by thirteen parameters, model estimation requires the search of a 13-dimensional parameter space. The 'curse of dimensionality,' compounded by a computationally intense forward problem, makes forward searches extremely time-consuming and frequently causes them to become trapped in local solutions. We find that both the speed and of the search can be improved by judiciously reducing the dimensionality of the search space. Our basic approach employs a hierarchy of models of increasing complexity that converges to the IBPES model. Earlier studies establish that a hierarchical sequence converges more quickly, and to a better solution, than a search relying only on the most complex model. Here we report results for a hierarchy of five models. The first three models treat the nebula as a 2D image, while the last two models explore its characteristics as a 3D object and enable us to characterize the physics of the nebula. This five-model hierarchy is applied to HST images of ellipsoidal PNe to estimate their geometric properties and gas density profiles.

  18. Bi-Abundance Ionisation Structure of the Wolf-Rayet Planetary Nebula PB 8 (United States)

    Danehkar, A.


    The planetary nebula PB 8 around a [WN/WC]-hybrid central star is one of planetary nebulae with moderate abundance discrepancy factors (ADFs 2-3), which could be an indication of a tiny fraction of metal-rich inclusions embedded in the nebula (bi-abundance). In this work, we have constructed photoionisation models to reproduce the optical and infrared observations of the planetary nebula PB 8 using a non-LTE stellar model atmosphere ionising source. A chemically homogeneous model initially used cannot predict the optical recombination lines. However, a bi-abundance model provides a better fit to most of the observed optical recombination lines from N and O ions. The metal-rich inclusions in the bi-abundance model occupy 5.6% of the total volume of the nebula, and are roughly 1.7 times cooler and denser than the mean values of the surrounding nebula. The N/H and O/H abundance ratios in the metal-rich inclusions are 1.0 and 1.7 dex larger than the diffuse warm nebula, respectively. To reproduce the Spitzer spectral energy distribution of PB 8, dust grains with a dust-to-gas ratio of 0.01 (by mass) were also included. It is found that the presence of metal-rich inclusions can explain the heavy element optical recombination lines, while a dual-dust chemistry with different grain species and discrete grain sizes likely produces the infrared continuum of this planetary nebula. This study demonstrates that the bi-abundance hypothesis, which was examined in a few planetary nebulae with large abundance discrepancies (ADFs > 10), could also be applied to those typical planetary nebulae with moderate abundance discrepancies.

  19. Interstellar Organics, the Solar Nebula, and Saturn's Satellite Phoebe (United States)

    Pendleton, Y. J.; Cruikshank, D. P.


    The diffuse interstellar medium inventory of organic material (Pendleton et al. 1994, Pendleton & Allamandola 2002) was likely incorporated into the molecular cloud in which the solar nebula condensed. This provided the feedstock for the formation of the Sun, major planets, and the smaller icy bodies in the region outside Neptune's orbit (transneptunian objects, or TNOs). Saturn's satellites Phoebe, Iapetus, and Hyperion open a window to the composition of one class of TNO as revealed by the near-infrared mapping spectrometer (VIMS) on the Cassini spacecraft at Saturn. Phoebe (mean diameter 213 km) is a former TNO now orbiting Saurn. VIMS spaectral maps of PHoebe's surface reveal a complex organic spectral signature consisting of prominent aromatic (CH) and alophatic hydrocarbon (CH2, CH3) absorption bands (3.2-3.6 micrometers). Phoebe is the source of a huge debris ring encircling Saturn, and from which particles (approximately 5-20 micrometer size) spiral inward toward Saturn. They encounter Iapetus and Hperion where they mix with and blanket the native H2O ice of those two bodies. Quantitative analysis of the hydrocarbon bands on Iapetus demonstrates that aromatic CH is approximately 10 times as abundant as aliphatic CH2+CH3, significantly exceeding the strength of the aromatic signature in interplanetary dust particles, comet particles, ad in carbonaceous meteorites (Cruikshank et al. 2013). A similar excess of aromatics over aliphatics is seen in the qualitative analysis of Hyperion and Phoebe itself (Dalle Ore et al. 2012). The Iapetus aliphatic hydrocarbons show CH2/CH3 approximately 4, which is larger than the value found in the diffuse ISM (approximately 2-2.5). In so far as Phoebe is a primitive body that formed in the outer regions of the solar nebula and has preserved some of the original nebula inventory, it can be key to understanding the content and degree of procesing of the nebular material. There are other Phoebe-like TNOs that are presently

  20. Nebula: reconstruction and visualization of scattering data in reciprocal space. (United States)

    Reiten, Andreas; Chernyshov, Dmitry; Mathiesen, Ragnvald H


    Two-dimensional solid-state X-ray detectors can now operate at considerable data throughput rates that allow full three-dimensional sampling of scattering data from extended volumes of reciprocal space within second to minute time-scales. For such experiments, simultaneous analysis and visualization allows for remeasurements and a more dynamic measurement strategy. A new software, Nebula, is presented. It efficiently reconstructs X-ray scattering data, generates three-dimensional reciprocal space data sets that can be visualized interactively, and aims to enable real-time processing in high-throughput measurements by employing parallel computing on commodity hardware.

  1. Mapping the Physical and Chemical Conditions of the Ring Nebula (United States)

    Leal-Ferreira, Marcelo L.; Aleman, Isabel; Gaughan, Andrea; Ladjal, Djazia; Ueta, Toshiya; Kerber, Samuel; Conn, Blair; Gardiner, Rhiannon; Tielens, Alexander G. G. M.


    We observed the Planetary Nebula NGC 6720 with the Gemini Telescope and the Gemini Multi-Object Spectrographs. We obtained spatial maps of 36 emission-lines in the wavelength range between 3600 Å and 9400 Å. We derived maps of c(Hβ), electronic densities, electronic temperatures, ionic and elemental abundances, and Ionization Correction Factors (ICFs) in the source and investigated the mass-loss history of the progenitor. The elemental abundance results indicate the need for ICFs based on three-dimensional photoionization models.

  2. On Calibrations Using the Crab Nebula as a Standard Candle (United States)

    Weisskopf, Martin; Guainazzi, Matteo; Jahoda, Keith; Shaposhnikov, Nikolai; ODell, Stephen; Zavlin, Vyacheslav; Wilson-Hodge, Colleen; Elsner, Ronald


    Inspired by a recent paper (Kirsch et al. 2005) on possible use of the Crab Nebula as a standard candle for calibrating X-ray response func tions, we examine possible consequences of intrinsic departures from a single (absorbed) power law upon such calibrations. We limited our analyses to three more modern X-ray instruments -- the ROSAT/PSPC, th e RXTE/PCA, and the XMM-Newton/EPIC-pn. The results are unexpected an d indicate a need to refine two of the three response functions studi ed. The implications for Chandra will be discussed.

  3. Planetary Nebulae as Probes of Dark Matter in NGC 3384


    Tremblay, Benoit; Merritt, David; Williams, T. B.


    We have obtained radial velocities of 68 planetary nebulae surrounding the SB0 galaxy NGC 3384 in the Leo I group, using the CTIO 4 m telescope and the Rutgers Fabry-Perot interferometer. The PN system exhibits a well-ordered rotation field aligned with the photometric axes of the galaxy. The rotation curve is flat from about 2 kpc until at least 7 kpc. Our results imply that at least a third of the dynamical mass of the NGC 3379/3384 system may be accounted for in the two bright galaxies.


    Energy Technology Data Exchange (ETDEWEB)

    Mochol, Iwona; Kirk, John G., E-mail:, E-mail: [Max-Planck-Institut fuer Kernphysik, Postfach 10 39 80, D-69029 Heidelberg (Germany)


    Nonlinear electromagnetic waves with superluminal phase velocity can propagate in the winds around isolated pulsars, and around some pulsars in binary systems. Using a short-wavelength approximation, we find and analyze an integrable system of equations that govern their evolution in spherical geometry. A confined mode is identified that stagnates to finite pressure at large radius and can form a precursor to the termination shock. Using a simplified criterion, we find this mode is stable for most isolated pulsars, but may be unstable if the external pressure is high, such as in the pulsar wind nebulae in starburst galaxies and in W44. Pulsar winds in eccentric binary systems, such as PSR 1259-63, may go through phases with stable and unstable electromagnetic precursors, as well as phases in which the density is too high for these modes to propagate.

  5. WT1, MSH6, GATA5 and PAX5 as epigenetic oral squamous cell carcinoma biomarkers - a short report. (United States)

    Ribeiro, Ilda Patrícia; Caramelo, Francisco; Marques, Francisco; Domingues, Ana; Mesquita, Margarida; Barroso, Leonor; Prazeres, Hugo; Julião, Maria José; Baptista, Isabel Poiares; Ferreira, Artur; Melo, Joana Barbosa; Carreira, Isabel Marques


    Oral squamous cell carcinoma (OSCC) is a frequently occurring aggressive malignancy with a heterogeneous clinical behavior. Based on the paucity of specific early diagnostic and prognostic biomarkers, which hampers the appropriate treatment and, ultimately the development of novel targeted therapies, we aimed at identifying such biomarkers through a genetic and epigenetic analysis of these tumors. 93 primary OSCCs were subjected to DNA copy number alteration (CNA) and methylation status analyses using methylation-specific multiplex ligation-dependent probe amplification (MS-MPLA). The genetic and epigenetic OSCC profiles obtained were associated with the patients' clinic-pathological features. We found that WT1 gene promoter methylation is a predictor of a better prognosis and that MSH6 and GATA5 gene promoter methylation serve as predictors of a worse prognosis. GATA5 gene promoter methylation was found to be significantly associated with a shorter survival rate. In addition, we found that PAX5 gene promoter methylation was significantly associated with tongue tumors. To the best of our knowledge, this is the first study that highlights this specific set of genes as epigenetic diagnostic and prognostic biomarkers in OSCC. Our data highlight the importance of epigenetically assessing OSCCs to identify key genes that may serve as diagnostic and prognostic biomarkers and, potentially, as candidate therapeutic targets.

  6. Constitutional mismatch repair deficiency and childhood leukemia/lymphoma--report on a novel biallelic MSH6 mutation. (United States)

    Ripperger, Tim; Beger, Carmela; Rahner, Nils; Sykora, Karl W; Bockmeyer, Clemens L; Lehmann, Ulrich; Kreipe, Hans H; Schlegelberger, Brigitte


    Biallelic mutations of mismatch repair genes cause constitutional mismatch repair deficiency associated with an increased risk for childhood leukemia/lymphoma. We report on a case with constitutional mismatch repair deficiency caused by a novel MSH6 mutation leading to a T-cell lymphoma and colonic adenocarcinoma at six and 13 years of age, respectively. A review of the literature on hematologic malignancies in constitutional mismatch repair deficiency showed that in almost half of the 47 known constitutional mismatch repair deficiency families, at least one individual is affected by a hematologic malignancy, predominantly T-cell lymphomas. However, diagnosing constitutional mismatch repair deficiency may be difficult when the first child is affected by leukemia/lymphoma, but identification of the causative germline mutation is of vital importance: (i) to identify relatives at risk and exclude an increased risk in non-mutation carriers; (ii) to prevent hematopoietic stem cell transplantation from sibling donors also carrying a biallelic germline mutation; and (iii) to implement effective surveillance programs for mutation carriers, that may reduce constitutional mismatch repair deficiency-associated mortality.

  7. Constitutional mismatch repair deficiency and childhood leukemia/lymphoma – report on a novel biallelic MSH6 mutation (United States)

    Ripperger, Tim; Beger, Carmela; Rahner, Nils; Sykora, Karl W.; Bockmeyer, Clemens L.; Lehmann, Ulrich; Kreipe, Hans H.; Schlegelberger, Brigitte


    Biallelic mutations of mismatch repair genes cause constitutional mismatch repair deficiency associated with an increased risk for childhood leukemia/lymphoma. We report on a case with constitutional mismatch repair deficiency caused by a novel MSH6 mutation leading to a T-cell lymphoma and colonic adenocarcinoma at six and 13 years of age, respectively. A review of the literature on hematologic malignancies in constitutional mismatch repair deficiency showed that in almost half of the 47 known constitutional mismatch repair deficiency families, at least one individual is affected by a hematologic malignancy, predominantly T-cell lymphomas. However, diagnosing constitutional mismatch repair deficiency may be difficult when the first child is affected by leukemia/lymphoma, but identification of the causative germline mutation is of vital importance: (i) to identify relatives at risk and exclude an increased risk in non-mutation carriers; (ii) to prevent hematopoietic stem cell transplantation from sibling donors also carrying a biallelic germline mutation; and (iii) to implement effective surveillance programs for mutation carriers, that may reduce constitutional mismatch repair deficiency-associated mortality. PMID:20015892

  8. 77 FR 29633 - Alta Wind VII, LLC, Alta Wind IX, LLC, Alta Wind X, LLC, Alta Wind XI, LLC, Alta Wind XII, LLC... (United States)


    ... Wind VII, LLC, Alta Wind IX, LLC, Alta Wind X, LLC, Alta Wind XI, LLC, Alta Wind XII, LLC, Alta Wind XIII, LLC, Alta Wind XIV, LLC, Alta Wind XV, LLC, Alta Windpower Development, LLC, TGP Development... 385.207, Alta Wind VII, LLC, Alta Wind IX, LLC, Alta Wind X, LLC, Alta Wind XI, LLC, Alta Wind XII...

  9. Adolescent binge-like ethanol exposure reduces basal α-MSH expression in the hypothalamus and the amygdala of adult rats. (United States)

    Lerma-Cabrera, Jose Manuel; Carvajal, Francisca; Alcaraz-Iborra, Manuel; de la Fuente, Leticia; Navarro, Montserrat; Thiele, Todd E; Cubero, Inmaculada


    Melanocortins (MC) are central peptides that have been implicated in the modulation of ethanol consumption. There is experimental evidence that chronic ethanol exposure reduces α-MSH expression in the limbic and hypothalamic brain regions and alters central pro-opiomelanocortin (POMC) mRNA activity in adult rats. Adolescence is a critical developmental period of high vulnerability in which ethanol exposure alters corticotropin releasing factor, neuropeptide Y, substance P and neurokinin neuropeptide activities, all of which have key roles in ethanol consumption. Given the involvement of MC and the endogenous inverse agonist AgRP in ethanol drinking, here we evaluate whether a binge-like pattern of ethanol treatment during adolescence has a relevant impact on basal and/or ethanol-stimulated α-MSH and AgRP activities during adulthood. To this end, adolescent Sprague-Dawley rats (beginning at PND25) were pre-treated with either saline (SP group) or binge-like ethanol exposure (BEP group; 3.0 g/kg given in intraperitoneal (i.p.) injections) of one injection per day over two consecutive days, followed by 2 days without injections, repeated for a total of 8 injections. Following 25 ethanol-free days, we evaluated α-MSH and AgRP immunoreactivity (IR) in the limbic and hypothalamic nuclei of adult rats (PND63) in response to ethanol (1.5 or 3.0 g/kgi.p.) and saline. We found that binge-like ethanol exposure during adolescence significantly reduced basal α-MSH IR in the central nucleus of the amygdala (CeA), the arcuate nucleus (Arc) and the paraventricular nucleus of the hypothalamus (PVN) during adulthood. Additionally, acute ethanol elicited AgRP IR in the Arc. Rats given the adolescent ethanol treatment required higher doses of ethanol than saline-treated rats to express AgRP. In light of previous evidence that endogenous MC and AgRP regulate ethanol intake through MC-receptor signaling, we speculate that the α-MSH and AgRP disturbances induced by binge


    Energy Technology Data Exchange (ETDEWEB)

    Schaefer, Bradley E.; Edwards, Zachary I. [Physics and Astronomy, Louisiana State University, Baton Rouge, LA 70803 (United States)


    Up until around 1980, the Stingray was an ordinary B1 post-AGB star, but then it suddenly sprouted bright emission lines like in a planetary nebula (PN), and soon after this the Hubble Space Telescope (HST) discovered a small PN around the star, so apparently we have caught a star in the act of ionizing a PN. We report here on a well-sampled light curve from 1889 to 2015, with unique coverage of the prior century plus the entire duration of the PN formation plus three decades of its aftermath. Surprisingly, the star anticipated the 1980s ionization event by declining from B = 10.30 in 1889 to B = 10.76 in 1980. Starting in 1980, the central star faded fast, at a rate of 0.20 mag year{sup −1}, reaching B = 14.64 in 1996. This fast fading is apparently caused by the central star shrinking in size. From 1994 to 2015, the V-band light curve is almost entirely from the flux of two bright [O iii] emission lines from the unresolved nebula, and it shows a consistent decline at a rate of 0.090 mag year{sup −1}. This steady fading (also seen in the radio and infrared) has a timescale equal to that expected for ordinary recombination within the nebula, immediately after a short-duration ionizing event in the 1980s. We are providing the first direct measure of the rapidly changing luminosity of the central star on both sides of a presumed thermal pulse in 1980, with this providing a strong and critical set of constraints, and these are found to sharply disagree with theoretical models of PN evolution.


    Energy Technology Data Exchange (ETDEWEB)

    Shacham, Tomer; Shaviv, Nir J. [Racah Institute of Physics, Hebrew University of Jerusalem, Jerusalem 91904 (Israel)


    We study the dynamics of continuum-driven winds from rotating stars and develop an approximate analytical model. We then discuss the evolution of stellar angular momentum, and show that just above the Eddington limit, the winds are sufficiently concentrated toward the poles to spin-up the star. A twin-lobe structure of the ejected nebula is seen to be a generic consequence of critical rotation. We find that if the pressure in such stars is sufficiently dominated by radiation, an equatorial ejection of mass will occur during eruptions. These results are then applied to {eta}-Carinae. We show that if it began its life with a high enough angular momentum, the present-day wind could have driven the star toward critical rotation, if it is the dominant mode of mass loss. We find that the shape and size of the Homunculus nebula, as given by our model, agree with recent observations. Moreover, the contraction expected due to the sudden increase in luminosity at the onset of the Great Eruption explains the equatorial 'skirt' as well.

  12. On Continuum-driven Winds from Rotating Stars (United States)

    Shacham, Tomer; Shaviv, Nir J.


    We study the dynamics of continuum-driven winds from rotating stars and develop an approximate analytical model. We then discuss the evolution of stellar angular momentum, and show that just above the Eddington limit, the winds are sufficiently concentrated toward the poles to spin-up the star. A twin-lobe structure of the ejected nebula is seen to be a generic consequence of critical rotation. We find that if the pressure in such stars is sufficiently dominated by radiation, an equatorial ejection of mass will occur during eruptions. These results are then applied to η-Carinae. We show that if it began its life with a high enough angular momentum, the present-day wind could have driven the star toward critical rotation, if it is the dominant mode of mass loss. We find that the shape and size of the Homunculus nebula, as given by our model, agree with recent observations. Moreover, the contraction expected due to the sudden increase in luminosity at the onset of the Great Eruption explains the equatorial "skirt" as well.

  13. Investigating CXOU J163802.6-471358: A New Pulsar Wind Nebula in the Norma Region? (United States)

    Jakobsen, Simone J.; Tomsick, John A.; Watson, Darach; Gotthelf, Eric V.; Kaspi, Victoria M.


    We present the first analysis of the extended source CXOU J163802.6-471358, which was discovered serendipitously during the Chandra X-ray survey of the Norma region of the Galactic spiral arms. The X-ray source exhibits a cometary appearance with a point source and an extended tail region. The complete source spectrum is fitted well with an absorbed power law model and jointly fitting the Chandra spectrum of the full source with one obtained from an archived XMM-Newton observation results in best fit parameters N H =1.5^{+0.7}_{-0.5}\\times 10^{23}\\, cm{^{-2}} and \\Gamma =1.1^{+0.7}_{-0.6} (90% confidence uncertainties). The unabsorbed luminosity of the full source is then L_X\\sim 4.8\\times 10^{33}d_{10}^2 erg s-1 with d 10 = d/10 kpc, where a distance of 10 kpc is a lower bound inferred from the large column density. The radio counterpart found for the source using data from the Molonglo Galactic Plane Survey epoch-2 shows an elongated tail offset from the X-ray emission. No infrared counterpart was found. The results are consistent with the source being a previously unknown pulsar driving a bow shock through the ambient medium.

  14. Two different sources of water for the early solar nebula. (United States)

    Kupper, Stefan; Tornow, Carmen; Gast, Philipp


    Water is essential for life. This is a trivial fact but has profound implications since the forming of life on the early Earth required water. The sources of water and the related amount of delivery depend not only on the conditions on the early Earth itself but also on the evolutionary history of the solar system. Thus we ask where and when water formed in the solar nebula-the precursor of the solar system. In this paper we explore the chemical mechanics for water formation and its expected abundance. This is achieved by studying the parental cloud core of the solar nebula and its gravitational collapse. We have identified two different sources of water for the region of Earth's accretion. The first being the sublimation of the icy mantles of dust grains formed in the parental cloud. The second source is located in the inner region of the collapsing cloud core - the so-called hot corino with a temperature of several hundred Kelvin. There, water is produced efficiently in the gas phase by reactions between neutral molecules. Additionally, we analyse the dependence of the production of water on the initial abundance ratio between carbon and oxygen.

  15. Observation and Spectral Measurements of the Crab Nebula with Milagro (United States)

    Abdo, A. A.; Allen, B. T.; Aune, T.; Benbow, W.; Berley, D.; Chen, C.; Christopher, G. E.; DeYoung, T.; Dingus, B. L.; Falcone, A.; hide


    The Crab Nebula was detected with the Milagro experiment at a statistical significance of 17 standard deviations over the lifetime of the experiment. The experiment was sensitive to approximately 100 GeV - 100 TeV gamma ray air showers by observing the particle footprint reaching the ground. The fraction of detectors recording signals from photons at the ground is a suitable proxy for the energy of the primary particle and has been used to measure the photon energy spectrum of the Crab Nebula between 1 and 100 TeV. The TeV emission is believed to be caused by inverse-Compton up-scattering scattering of ambient photons by an energetic electron population. The location of a Te V steepening or cutoff in the energy spectrum reveals important details about the underlying electron population. We describe the experiment and the technique for distinguishing gamma-ray events from the much more-abundant hadronic events. We describe the calculation of the significance of the excess from the Crab and how the energy spectrum is fit.

  16. The Integral Field View of the Orion Nebula

    Directory of Open Access Journals (Sweden)

    Adal Mesa-Delgado


    Full Text Available This paper reviews the major advances achieved in the Orion Nebula through the use of integral field spectroscopy (IFS. Since the early work of Vasconcelos and collaborators in 2005, this technique has facilitated the investigation of global properties of the nebula and its morphology, providing new clues to better constrain its 3D structure. IFS has led to the discovery of shock-heated zones at the leading working surfaces of prominent Herbig-Haro objects as well as the first attempt to determine the chemical composition of Orion protoplanetary disks, also known as proplyds. The analysis of these morphologies using IFS has given us new insights into the abundance discrepancy problem, a long-standing and unresolved issue that casts doubt on the reliability of current methods used for the determination of metallicities in the universe from the analysis of H II regions. Results imply that high-density clumps and high-velocity flows may play an active role in the production of such discrepancies. Future investigations based on the large-scale IFS mosaic of Orion will be very valuable for exploring how the integrated effect of small-scale structures may have impact at larger scales in the framework of star-forming regions.


    Directory of Open Access Journals (Sweden)

    Barladean A.S.


    Full Text Available The problem of wind turbine choice for wind power stations is examined in this paper. It is shown by comparison of parameters and characteristics of wind turbines, that for existing modes and speeds of wind in territory of Republic of Moldova it is necessary to use multi-blade small speed rotation wind turbines of fan class.

  18. Wind turbine (United States)

    Cheney, Jr., Marvin C.


    A wind turbine of the type having an airfoil blade (15) mounted on a flexible beam (20) and a pitch governor (55) which selectively, torsionally twists the flexible beam in response to wind turbine speed thereby setting blade pitch, is provided with a limiter (85) which restricts unwanted pitch change at operating speeds due to torsional creep of the flexible beam. The limiter allows twisting of the beam by the governor under excessive wind velocity conditions to orient the blades in stall pitch positions, thereby preventing overspeed operation of the turbine. In the preferred embodiment, the pitch governor comprises a pendulum (65,70) which responds to changing rotor speed by pivotal movement, the limiter comprising a resilient member (90) which engages an end of the pendulum to restrict further movement thereof, and in turn restrict beam creep and unwanted blade pitch misadjustment.

  19. The central effects of alpha-melanocyte stimulating hormone (α-MSH) in chicks involve changes in gene expression of neuropeptide Y and other factors in distinct hypothalamic nuclei. (United States)

    Delp, Meghan S; Cline, Mark A; Gilbert, Elizabeth R


    Alpha-melanocyte stimulating hormone (α-MSH) is a satiety-inducing factor in birds and mammals although central mechanisms mediating its effects on appetite in birds are poorly understood. Thus, the objective of the present study was to determine effects of centrally-injected α-MSH on c-Fos and gene expression in chick appetite-associated hypothalamic nuclei. At 4days post-hatch, 3h-fasted chicks were intracerebroventricularly (ICV) injected with 0 (vehicle) or 0.12nmol α-MSH and 1h later, hypothalamus samples were collected for measuring c-Fos immunoreactivity and mRNA abundance of appetite-associated factors in hypothalamic nuclei. There were more c-Fos immunoreactive cells in the arcuate nucleus (ARC), dorsomedial nucleus (DMN), lateral hypothalamus (LH), and paraventricular nucleus (PVN) of α-MSH- than vehicle-injected chicks. Neuropeptide Y (NPY), oxytocin receptor (OXTR), and agouti-related peptide (AgRP) mRNAs were greater in α-MSH- than vehicle-injected chicks in the ARC. In the PVN, NPY receptor sub-type 1 (NPYR1) mRNA was reduced while c-Fos mRNA was increased in response to treatment with α-MSH. NPY, c-Fos, and DOPA decarboxylase (DDC) mRNAs were greater in treated than vehicle-injected chicks in the DMN. Results suggest that during the first hour post-injection, the appetite-inhibiting effects of α-MSH involve activation of the ARC, DMN, PVN, and LH, and corresponding changes in transcriptional regulation of factors involved with NPY, AgRP and mesotocin signaling, and monoamine synthesis. The effects of these changes may include an inhibition of NPY signaling in the PVN to induce satiety and stimulation of NPY/AgRP neurons in the ARC in an attempt to restore homeostatic levels of food intake. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Characterization of the optical and X-ray properties of the north-western wisps in the Crab nebula (United States)

    Schweizer, T.; Bucciantini, N.; Idec, W.; Nilsson, K.; Tennant, A.; Weisskopf, M. C.; Zanin, R.


    We have studied the wisps to the north-west of the Crab pulsar as part of a multiwavelength campaign in the visible and in X-rays. Optical observations were obtained using the Nordic Optical Telescope in La Palma and X-ray observations were made with the Chandra X-ray Observatory. The observing campaign took place from 2010 October until 2012 September. About once per year we observe wisps forming and peeling off from (or near) the region commonly associated with the termination shock of the pulsar wind. We find that the exact locations of the north-western wisps in the optical and in X-rays are similar but not coincident, with X-ray wisps preferentially located closer to the pulsar. This suggests that the optical and X-ray wisps are not produced by the same particle distribution. Our measurements and their implications are interpreted in terms of a Doppler-boosted ring model that has its origin in magnetohydrodynamic (MHD) modelling. While the Doppler boosting factors inferred from the X-ray wisps are consistent with current MHD simulations of pulsar wind nebulae, the optical boosting factors are not, and typically exceed values from MHD simulations by about a factor of 3.

  1. A Self-Perpetuating Catalyst for the Production of Complex Organic Molecules in Protostellar Nebulae (United States)

    Nuth, Joseph A.; Johnson, N. M.


    The formation of abundant carbonaceous material in meteorites is a long standing problem and an important factor in the debate on the potential for the origin of life in other stellar systems. Many mechanisms may contribute to the total organic content in protostellar nebulae, ranging from organics formed via ion-molecule and atom-molecule reactions in the cold dark clouds from which such nebulae collapse, to similar ion-molecule and atom-molecule reactions in the dark regions of the nebula far from the proto star, to gas phase reactions in sub-nebulae around growing giant planets and in the nebulae themselves. The Fischer-Tropsch-type (FTT) catalytic reduction of CO by hydrogen was once the preferred model for production of organic materials in the primitive solar nebula. The Haber-Bosch catalytic reduction of N2 by hydrogen was thought to produce the reduced nitrogen found in meteorites. However, the clean iron metal surfaces that catalyze these reactions are easily poisoned via reaction with any number of molecules, including the very same complex organics that they produce and both reactions work more efficiently in the hot regions of the nebula. We have demonstrated that many grain surfaces can catalyze both FTT and HB-type reactions, including amorphous iron and magnesium silicates, pure silica smokes as well as several minerals. Although none work as well as pure iron grains, and all produce a wide range of organic products rather than just pure methane, these materials are not truly catalysts.

  2. Registration of H2O and SiO masers in the Calabash Nebula, to confirm the Planetary Nebula paradigm (United States)

    Dodson, R.; Rioja, M.; Bujarrabal, V.; Kim, J.; Cho, SH; Choi, YK; Youngjoo, Y.


    We report on the astrometric registration of VLBI images of the SiO and H2O masers in OH 231.8+4.2, the iconic Proto-Planetary Nebula also known as the Calabash nebula, using the KVN and Source/Frequency Phase Referencing. This, for the first time, robustly confirms the alignment of the SiO masers, close to the AGB star, driving the bi-lobe structure with the water masers in the out-flow. We are able to trace the bulk motions for the H2O masers over the last few decades to be 19 km s-1 and deduce that the age of this expansion stage is 38±2 years. The combination of this result with the distance allows a full 3D reconstruction, and confirms that the H2O masers lie on and expand along the known large-scale symmetry axis and that the outflow is only a few decades old, so mass loss is almost certainly on-going. Therefore we conclude that the SiO emission marks the stellar core of the nebular, the H2O emission traces the expansion, and that there must be multiple epochs of ejection to drive the macro-scale structure.

  3. Remote Sensing Wind and Wind Shear System. (United States)

    Contents: Remote sensing of wind shear and the theory and development of acoustic doppler; Wind studies; A comparison of methods for the remote detection of winds in the airport environment; Acoustic doppler system development; System calibration; Airport operational tests.

  4. Spitzer Observations of MF 16 Nebula and the Associated Ultraluminous X-Ray Source (United States)


    reserved. Printed in the U.S.A. SPITZER OBSERVATIONS OF MF 16 NEBULA AND THE ASSOCIATED ULTRALUMINOUS X-RAY SOURCE C. T. Berghea and R. P. Dudik United...associated nebula MF 16. This ULX has very similar properties to the famous Holmberg II ULX, the first ULX to show a prominent infrared [O iv] emission...the most interesting developments in ULX history is the discovery in recent years of large ionized bubble nebulae around some of the most famous ULXs

  5. Wind Energy Japan

    Energy Technology Data Exchange (ETDEWEB)

    Komatsubara, Kazuyo [Embassy of the Kingdom of the Netherlands, Tokyo (Japan)


    An overview is given of wind energy in Japan: Background; Wind Energy in Japan; Japanese Wind Energy Industry; Government Supports; Useful Links; Major Japanese Companies; Profiles of Major Japanese Companies; Major Wind Energy Projects in Japan.

  6. Comparison of energy efficiency between Wearable Power-Assist Locomotor (WPAL) and two types of knee-ankle-foot orthoses with a medial single hip joint (MSH-KAFO). (United States)

    Yatsuya, Kanan; Hirano, Satoshi; Saitoh, Eiichi; Tanabe, Shigeo; Tanaka, Hirotaka; Eguchi, Masayuki; Katoh, Masaki; Shimizu, Yasuhiro; Uno, Akito; Kagaya, Hitoshi


    To compare the energy efficiency of Wearable Power-Assist Locomotor (WPAL) with conventional knee-ankle-foot orthoses (MSH-KAFO) such as Hip and Ankle Linked Orthosis (HALO) or Primewalk. Cross over case-series. Chubu Rosai Hospital, Aichi, Japan, which is affiliated with the Japan Organization of Occupational Health and Safety. Six patients were trained with MSH-KAFO (either HALO or Primewalk) and WPAL. They underwent 6-minute walk tests with each orthosis. Energy efficiency was estimated using physiological cost index (PCI) as well as heart rate (HR) and modified Borg score. Trial energy efficiency with MSH-KAFO was compared with WPAL to assess if differences in PCI became greater between MSH-KAFO and WPAL as time goes on during the 6-minute walk. Spearman correlation coefficient of time (range: 0.5-6.0 minutes) with the difference was calculated. The same statistical procedures were repeated for HR and modified Borg score. Greater energy efficiency, representing a lower gait demand, was observed in trials with WPAL compared with MSH-KAFO (Spearman correlation coefficients for PCI, HR and modified Borg were 0.93, 0.90 and 0.97, respectively, all P energy efficient type of robotics that may be used by patients with paraplegia.

  7. A test for asymptotic giant branch evolution theories: planetary nebulae in the Large Magellanic Cloud (United States)

    Ventura, P.; Stanghellini, L.; Dell'Agli, F.; García-Hernández, D. A.; Di Criscienzo, M.


    We used a new generation of asymptotic giant branch (AGB) stellar models that include dust formation in the stellar winds to find the links between evolutionary models and the observed properties of a homogeneous sample of Large Magellanic Cloud (LMC) planetary nebulae (PNe). Comparison between the evolutionary yields of elements such as CNO and the corresponding observed chemical abundances is a powerful tool to shed light on evolutionary processes such as hot bottom burning (HBB) and third dredge-up (TDU). We found that the occurrence of HBB is needed to interpret the nitrogen-enriched (log (N/H) + 12 > 8) PNe. In particular, N-rich PNe with the lowest carbon content are nicely reproduced by AGB models of mass M ≥ 6 M⊙, whose surface chemistry reflects the pure effects of HBB. PNe with log (N/H) + 12 HBB, with initial mass below ˜3 M⊙. Some of these stars show very large carbon abundances, owing to the many TDU episodes experienced. We found from our LMC PN sample that there is a threshold to the amount of carbon accumulated at AGB surfaces, log (C/H) + 12 < 9. Confirmation of this constraint would indicate that, after the C-star stage is reached, AGBs experience only a few thermal pulses, which suggests a rapid loss of the external mantle, probably owing to the effects of radiation pressure on carbonaceous dust particles present in the circumstellar envelope. The implications of these findings for AGB evolution theories and the need to extend the PN sample currently available are discussed.

  8. Challenges faced by current Galactic planetary nebula surveys (United States)

    Frew, David J.


    Determining the demographics of the Galactic planetary nebula (PN) population is an important goal to further our understanding of this intriguing phase of stellar evolution. The Galactic population has more than doubled in number over the last 15 years, particularly from narrowband Hα surveys along the plane. In this review I will summarise these results, with emphasis on the time interval since the last IAU Symposium. These primarily optical surveys are not without their limitations and new surveys for PNe in the infrared similarly face a number of challenges. I will discuss the need for multi-wavelength approaches to discovery and analysis. The desire to have accurate volume-limited samples of Galactic PNe at our disposal is emphasised, which will be impacted with new data from the Gaia satellite mission. We need robust surveys of PNe and their central stars, especially volume-limited surveys, in order to clarify and quantify their evolutionary pathways.

  9. Galactic planetary nebulae as absolute probes: The view from Gaia (United States)

    Stanghellini, Letizia; Bucciarelli, Beatrice; Lattanzi, Mario G.; Morbidelli, Roberto


    We searched the first Gaia data release for Galactic central stars of planetary nebulae (CSPNe) for parallaxes in order to determine the distances of the hosting PNe. For the small sample of PNe for which a comparison is available, we show that distances derived from Gaia parallaxes agree, within the uncertainties, with the individual PN distances derived by other reliable methods. While Gaia parallaxes available for Galactic CSPNe are still few, and with high uncertainties, we studied the possibility of building a PN distance scale by using the Gaia distances as calibrators. We found that a scale built on the relation between the linear nebular radius and its surface brightness has promising future applications.

  10. Spectroscopic Binaries in the Orion Nebula Cluster and NGC 2264 (United States)

    Kounkel, Marina; Hartmann, Lee; Tobin, John J.; Mateo, Mario; Bailey, John I., III; Spencer, Meghin


    We examine the spectroscopic binary population for two massive nearby regions of clustered star formation, the Orion Nebula Cluster (ONC) and NGC 2264, supplementing the data presented by Tobin et al. with more recent observations and more extensive analysis. The inferred multiplicity fraction up to 10 au based on these observations is 5.3 ± 1.2% for NGC 2264 and 5.8 ± 1.1% for the ONC; these values are consistent with the distribution of binaries in the field in the relevant parameter range. Eight of the multiple systems in the sample have enough epochs to perform an initial fit for the orbital parameters. Two of these sources are double-lined spectroscopic binaries; for them, we determine the mass ratio. Our reanalysis of the distribution of stellar radial velocities toward these clusters presents a significantly better agreement between stellar and gas kinematics than was previously thought.

  11. The ultraviolet extinction properties of the 30 Dor Nebula (United States)

    De Marchi, Guido; Panagia, Nino


    Recent investigation of the extinction law in 30 Dor and the Tarantula Nebula, at optical and near infrared wavelengths, has revealed a ratio of total to selective extinction RV=AV/E(B-V) of about 4.5. This indicates a larger proportion of large grains than in the Galactic diffuse interstellar medium. Possible origins include coalescence of small grains, grain growth, selective destruction of small grains, and fresh injection of large grains. From a study of the ultraviolet extinction properties of three Wolf-Rayet stars in 30 Dor (R 139, R 140, R 145), observed with the International Ultraviolet Explorer, we show that the excess of large grains does not come at the expense of small grains, which are still present. Fresh injection of large grains by supernova explosions appears to be the dominant mechanism.


    Energy Technology Data Exchange (ETDEWEB)

    Kounkel, Marina; Hartmann, Lee; Mateo, Mario; Bailey, John I. III; Spencer, Meghin [Department of Astronomy, University of Michigan, 1085 S. University Street, Ann Arbor, MI 48109 (United States); Tobin, John J., E-mail: [Leiden Observatory, Leiden University, P.O. Box 9513, 2300-RA Leiden (Netherlands)


    We examine the spectroscopic binary population for two massive nearby regions of clustered star formation, the Orion Nebula Cluster (ONC) and NGC 2264, supplementing the data presented by Tobin et al. with more recent observations and more extensive analysis. The inferred multiplicity fraction up to 10 au based on these observations is 5.3 ± 1.2% for NGC 2264 and 5.8 ± 1.1% for the ONC; these values are consistent with the distribution of binaries in the field in the relevant parameter range. Eight of the multiple systems in the sample have enough epochs to perform an initial fit for the orbital parameters. Two of these sources are double-lined spectroscopic binaries; for them, we determine the mass ratio. Our reanalysis of the distribution of stellar radial velocities toward these clusters presents a significantly better agreement between stellar and gas kinematics than was previously thought.

  13. Planetary nebulae as kinematic tracers of galaxy stellar halos (United States)

    Coccato, Lodovico


    The kinematic and dynamical properties of galaxy stellar halos are difficult to measure because of the faint surface brightness that characterizes these regions. Spiral galaxies can be probed using the radio Hi emission; on the contrary, early-type galaxies contain less gas, therefore alternative kinematic tracers need to be used. Planetary nebulae (PNe) can be easily detected far out in the halo thanks to their bright emission lines. It is therefore possible to map the halo kinematics also in early-type galaxies, typically out to 5 effective radii or beyond. Thanks to the recent spectroscopic surveys targeting extra-galactic PNe, we can now rely on a few tens of galaxies where the kinematics of the stellar halos are measured. Here, I will review the main results obtained in this field in the last decades.

  14. Volatile inventories in clathrate hydrates formed in the primordial nebula. (United States)

    Mousis, Olivier; Lunine, Jonathan I; Picaud, Sylvain; Cordier, Daniel


    The examination of ambient thermodynamic conditions suggests that clathrate hydrates could exist in the Martian permafrost, on the surface and in the interior of Titan, as well as in other icy satellites. Clathrate hydrates are probably formed in a significant fraction of planetesimals in the solar system. Thus, these crystalline solids may have been accreted in comets, in the forming giant planets and in their surrounding satellite systems. In this work, we use a statistical thermodynamic model to investigate the composition of clathrate hydrates that may have formed in the primordial nebula. In our approach, we consider the formation sequence of the different ices occurring during the cooling of the nebula, a reasonable idealization of the process by which volatiles are trapped in planetesimals. We then determine the fractional occupancies of guests in each clathrate hydrate formed at a given temperature. The major ingredient of our model is the description of the guest-clathrate hydrate interaction by a spherically averaged Kihara potential with a nominal set of parameters, most of which are fitted to experimental equilibrium data. Our model allows us to find that Kr, Ar and N2 can be efficiently encaged in clathrate hydrates formed at temperatures higher than approximately 48.5 K in the primitive nebula, instead of forming pure condensates below 30 K. However, we find at the same time that the determination of the relative abundances of guest species incorporated in these clathrate hydrates strongly depends on the choice of the parameters of the Kihara potential and also on the adopted size of cages. Indeed, by testing different potential parameters, we have noted that even minor dispersions between the different existing sets can lead to non-negligible variations in the determination of the volatiles trapped in clathrate hydrates formed in the primordial nebula. However, these variations are not found to be strong enough to reverse the relative abundances

  15. New Observations of the Crab Nebula and Pulsar (United States)

    Weisskopf, Martin C.; Tennant, Allyn F.; ODell, Stephen L.; Elsner, Ronald f.; Yakovlev, Dmitry R.; Zavlin, Vyacheslav E.; Becker, Werner


    We present a phase-resolved study of the X-ray spectrum of the Crab Pulsar, using data obtained in a special mode with the Chandra X-ray Observatory. The superb angular resolution easily enables discerning the Pulsar from the surrounding nebulosity, even at pulse minimum. We find that the Pulsar's X-ray spectral index varies sinusoidally with phase---except over the same phase range for which rather abrupt changes in optical polarization magnitude and position angle have been reported. In addition, we use the X-ray data to constrain the surface temperature for various neutron-star equations of state and atmospheres. Finally, we present new data on dynamical variations of structure within the Nebula.

  16. Deuterium enrichment in the primitive ices of the protosolar nebula (United States)

    Lutz, Barry L.; Owen, Tobias; De Bergh, Catherine


    On the basis of CH3D/CH4-ratio observations in the outer planets, the present effort to estimate the D/H ratio of the protosolar nebula's primitive ices arrives at two simple, yet effectively limiting models which constrain the degree of dilution undergone by deuterated volatiles through mixing with the initial hydrogen envelopes. These volatiles would have been contributed to planetary atmospheres by evaporated primordial ices. Ice D/H ratio model results of 0.0001 to 0.001 are compared with values for other potentially primitive material-containing bodies in the solar system, as well as with D/H ratio values from interstellar polyatomic molecules.

  17. a Surprise from the Pulsar in the Crab Nebula (United States)


    New observations of the spectrum of the rapidly spinning neutron star (the `pulsar') in the Crab Nebula have been carried out with the ESO 3.5-metre New Technology Telescope (NTT) by a group of Italian astronomers [1]. Because of greatly improved spectral resolution which allows to register even very fine details in the pulsar's spectrum, they are able to determine for the first time with high accuracy the overall dependance of the emission on wavelength, i.e. the `shape' of the spectrum. Quite unexpectedly, they also detect a hitherto unknown 100 A (10 nm) broad `absorption dip', which can be securely attributed to the pulsar. These results open an exciting new window for the study of the extreme physical processes close to a pulsar. The Nature of Pulsars It is estimated that there may be as many as 100 million neutron stars in our Galaxy. A neutron star is the superdense remnant of the extremely violent supernova explosion that occurs at the end of the life of a comparatively massive star. In fact, all stars that are more than about 6 times heavier than the Sun are believed to end their lives as supernovae. During the explosion, the central core of the dying star collapses in a few milliseconds and the matter at the centre is compressed to a density comparable to that of an atomic nucleus. Due to the enormous inward pressure, the atomic particles are squeezed together into a kind of neutron jam. The outcome is the formation of a neutron star with a diameter of 10-15 kilometres, weighing as much as the Sun. In accordance with the physical law that implies that the rotation momentum of the exploding star must be conserved, newborn neutron stars will rotate very rapidly around their axis, in some cases as fast as 100 times per second. In the same way, the new neutron star is expected to possess a strong magnetic field. Of these myriads of neutron stars, about 700 have been observed to emit radio pulses (hence the name `pulsar'). A few of these can also be detected

  18. Resolving the Disk-Halo Degeneracy using Planetary Nebulae (United States)

    Aniyan, S.; Freeman, K. C.; Arnaboldi, M.; Gerhard, O.; Coccato, L.; Fabricius, M.; Kuijken, K.; Merrifield, M.


    The decomposition of the 21 cm rotation curve of galaxies into contribution from the disk and dark halo depends on the adopted mass to light ratio (M/L) of the disk. Given the vertical velocity dispersion (σ z ) of stars in the disk and its scale height (h z ), the disk surface density and hence the M/L can be estimated. Earlier works have used this technique to conclude that galaxy disks are submaximal. Here we address an important conceptual problem: star-forming spirals have an old (kinematically hot) disk population and a young cold disk population. Both of these populations contribute to the integrated light spectra from which σ z is measured. The measured scale height h z is for the old disk population. In the Jeans equation, σ z and h z must pertain to the same population. We have developed techniques to extract the velocity dispersion of the old disk from integrated light spectra and from samples of planetary nebulae. We present the analysis of the disk kinematics of the galaxy NGC 628 using IFU data in the inner regions and planetary nebulae as tracers in the outer regions of the disk. We demonstrate that using the scale height of the old thin disk with the vertical velocity dispersion of the same population, traced by PNe, results in a maximal disk for NGC 628. Our analysis concludes that previous studies underestimate the disk surface mass density by ~ 2, sufficient to make a maximal disk for NGC 628 appear like a submaximal disk.

  19. Synthesis of Organics in the Early Solar Nebula (United States)

    Johnson, Natasha M.; Manning, S.; Nuth, J. A., III


    It is unknown what process or processes made the organics that are found or detected in extraterrestrial materials. One process that forms organics are Fischer-Tropsch type (FTT) reactions. Fischer-Tropsch type synthesis produces complex hydrocarbons by hydrogenating carbon monoxide via surface mediated reactions. The products of these reactions have been well-studied using `natural’ catalysts [1] and calculations of the efficiency of FTT synthesis in the Solar Nebula suggest that these types of reactions could make significant contributions to material near three AU [2]. We use FTT synthesis to coat Fe-silicate amorphous grains with organic material to simulate the chemistry in the early Solar Nebula. These coatings are composed of macromolecular organic phases [3]. Previous work also showed that as the grains became coated, Haber-Bosch type reactions took place resulting in nitrogen-bearing organics [4]. Our experiments consist of circulating CO, N2, and H2 gas through Fe- amorphous silicate grains that are maintained at a specific temperature in a closed system. The gases are passed through an FTIR spectrometer and are measured to monitor the reaction progress. Samples are analyzed using FTIR, and GCMS (including pyrolysis) and extraction techniques are used to analyze the organic coatings. These experiments show that these types of reactions are an effective means to produce complex hydrocarbons. We present the analysis of the produced organics (solid and gas phase) and the change in the production rate of several compounds as the grains become coated. Organics generated by this technique could represent the carbonaceous material incorporated in comets and meteorites. References: [1] Hayatsu and Anders 1981. Topics in Current Chemistry 99:1-37. [2] Kress and Tielens 2001. MAPS 36:75-91. [3] Johnson et al. 2004. #1876. 35th LPSC. [4] Hill and Nuth 2003. Astrobiology 3:291-304. This work was supported by a grant from NASA.

  20. Cloud structure and feedback effects in the Carina Nebula Complex (United States)

    Roccatagliata, Veronica; Preibisch, Thomas; Gaczkowski, Benjamin; Ratzka, Thorsten


    The star formation process in large clusters/associations can be strongly influenced by the feedback from high mass stars. Whether the resulting net effect of the feedback is predominantly negative (cloud dispersal) or positive (triggering of star formation due to cloud compression) is still an open question. The Carina Nebula complex (CNC) represents one of the most massive star-forming regions in our Galaxy. We use our Herschel far-infrared observations to study the properties of the clouds over the entire CNC and LABOCA/APEX telescope on the central part of the CNC.Our Herschel maps resolve, for the first time, the small-scale structure of the dense clouds over the entire spatial extent of the CNC. Several particularly interesting regions, including the prominent pillars south of eta Car, are analyzed in detail. Our maps also reveal a peculiar 'wave'-like pattern in the northern part of the Carina Nebula. The total mass of the clouds seen by Herschel in the central region is about 656 000 Msun. We derive the global spectral energy distribution in the mid-infrared to mm wavelength range and derive a total mass of stars, rather than random turbulence. Comparing the cloud mass and the star formation rate derived for the CNC to other Galactic star forming regions suggests that the CNC is forming stars very efficiently. We suggest this to be a consequence of triggered star formation by radiative cloud compression.In our LABOCA sub-mm map, we identify about 600 individual clumps. We analyze and interpret the clump initial mass function (CIMF) as signature of turbulent pre-stellar clouds or star-forming clouds.

  1. Human MLH1 protein participates in genomic damage checkpoint signaling in response to DNA interstrand crosslinks, while MSH2 functions in DNA repair.

    Directory of Open Access Journals (Sweden)

    Qi Wu


    Full Text Available DNA interstrand crosslinks (ICLs are among the most toxic types of damage to a cell. For this reason, many ICL-inducing agents are effective therapeutic agents. For example, cisplatin and nitrogen mustards are used for treating cancer and psoralen plus UVA (PUVA is useful for treating psoriasis. However, repair mechanisms for ICLs in the human genome are not clearly defined. Previously, we have shown that MSH2, the common subunit of the human MutSalpha and MutSbeta mismatch recognition complexes, plays a role in the error-free repair of psoralen ICLs. We hypothesized that MLH1, the common subunit of human MutL complexes, is also involved in the cellular response to psoralen ICLs. Surprisingly, we instead found that MLH1-deficient human cells are more resistant to psoralen ICLs, in contrast to the sensitivity to these lesions displayed by MSH2-deficient cells. Apoptosis was not as efficiently induced by psoralen ICLs in MLH1-deficient cells as in MLH1-proficient cells as determined by caspase-3/7 activity and binding of annexin V. Strikingly, CHK2 phosphorylation was undetectable in MLH1-deficient cells, and phosphorylation of CHK1 was reduced after PUVA treatment, indicating that MLH1 is involved in signaling psoralen ICL-induced checkpoint activation. Psoralen ICLs can result in mutations near the crosslinked sites; however, MLH1 function was not required for the mutagenic repair of these lesions, and so its signaling function appears to have a role in maintaining genomic stability following exposure to ICL-induced DNA damage. Distinguishing the genetic status of MMR-deficient tumors as MSH2-deficient or MLH1-deficient is thus potentially important in predicting the efficacy of treatment with psoralen and perhaps with other ICL-inducing agents.

  2. Mutation and association analyses of the candidate genes ESR1, ESR2, MAX, PCNA, and KAT2A in patients with unexplained MSH2-deficient tumors. (United States)

    Rahner, Nils; Brockschmidt, Felix F; Steinke, Verena; Kahl, Philip; Becker, Tim; Vasen, Hans F A; Wijnen, Juul T; Tops, Carli J M; Holinski-Feder, Elke; Ligtenberg, Marjolijn J L; Spruijt, Liesbeth; Görgens, Heike; Stemmler, Susanne; Kloor, Matthias; Dietmaier, Wolfgang; Schumacher, Johannes; Nöthen, Markus M; Propping, Peter


    Lynch syndrome (Hereditary non-polyposis colorectal cancer/HNPCC) is a cancer susceptibility syndrome which is caused by germline mutations in DNA mismatch repair (MMR) genes, in particular MLH1 and MSH2. A pathogenic germline mutation in the respective MMR gene is suggested by the finding of a loss of a mismatch repair protein in tumor tissue on immunohistochemical staining combined with an early age of onset and/or the familial occurrence of colorectal cancer. Pathogenic germline mutations are identifiable in around 60% of patients suspected of Lynch syndrome, depending on the familial occurrence. The aim of the present study was to identify novel susceptibility genes for Lynch syndrome. 64 Healthy controls and 64 Lynch syndrome patients with no pathogenic MSH2 mutation but a loss of MSH2 expression in their tumor tissue were screened for rare and disease causing germline mutations in the functional candidate genes ESR1, ESR2, MAX, PCNA, and KAT2A. Thirty variants were identified, and these were then genotyped in an independent sample of 36 mutation negative Lynch syndrome patients and 234 controls. Since a trend towards association was observed for KAT2A, an additional set of 21 tagging SNPs was analyzed at this locus in a final case-control sample of 142 mutation negative Lynch syndrome patients and 298 controls. The mutation analysis failed to reveal any rare disease-causing mutations. No association was found at the single-marker or haplotypic level for any common disease-modifying variant. The present results suggest that neither rare nor common genetic variants in ESR1, ESR2, MAX, PCNA, or KAT2A contribute to the development of Lynch syndrome.

  3. The Draco Nebula, a Molecular Cloud Associated with a High Velocity Cloud? (United States)

    Mebold, U.; Kalberla, P. W. M.


    Extended and very faint bright nebulae are found in high galactic latitudes at the Palomar Observatory Sky Survey. Such a nebula, located in the constellation Draco and called Draco Nebula or Dracula, was found to be in detailed positional coincidence with a 21 cm emission line feature. Estimates of the minimum visual extinction from star counts ON and OFF Dracula and an estimated visual surface brightness indicate that Dracula fits the relation SBV = 24.2 - 2.5 log AV for dust clouds located above the galactic plane and reflecting the integrated starlight of the galactic disk. Hence Dracula is probably a reflection nebula. Indicators of molecular hydrogen in Dracula, molecules such as CO, were searched for by using a 2.5-m mm-telescope. Molecular hydrogen column densities were estimated. The dynamics of CO clumps was studied. Dracula has a close positional and possibly even astrophysical relationship to the high velocity cloud phenomenon.

  4. Draco Nebula, a molecular cloud associated with a high velocity cloud

    Energy Technology Data Exchange (ETDEWEB)

    Mebold, U.; Kalberla, P.W.M.


    Extended and very faint bright nebulae are found in high galactic latitudes at the Palomar Observatory Sky Survey. Such a nebula, located in the constellation Draco and called Draco Nebula or Dracula, was found to be in detailed positional coincidence with a 21 cm emission line feature. Estimates of the minimum visual extinction from star counts ON and OFF Dracula and an estimated visual surface brightness indicate that Dracula fits the relation SBV 24.2 - 2.5 log AV for dust clouds located above the galactic plane and reflecting the integrated starlight of the galactic disk. Hence Dracula is probably a reflection nebula. Indicators of molecular hydrogen in Dracula, molecules such as CO, were searched for by using a 2.5-m mm-telescope. Molecular hydrogen column densities were estimated. The dynamics of CO clumps was studied. Dracula has a close positional and possibly even astrophysical relationship to the high velocity cloud phenomenon.

  5. Water masers and ammonia (1, 1) and (2, 2) towards six regions in the Carina Nebula (United States)

    Breen, S. L.; Green, C.-E.; Cunningham, M. R.; Voronkov, M. A.; Horiuchi, S.; Green, J. A.


    We present water maser and ammonia (1, 1) and (2, 2) observations, towards six regions in the Carina Nebula, conducted with the Australia Telescope Compact Array. In total five water masers were detected within two of the target fields, and we provide their accurate positions and characteristics. These five water masers constitute all of the known masers detected towards star formation regions in the Carina Nebula and we argue, that given the evidence for active star formation, and the presence of many high-mass stars, the Carina Nebula is uncharacteristically devoid of masers. Our results are consistent with the Carina Nebula having a lack of young high-mass stars, despite the presence of older high-mass stars. Ammonia (1, 1) and (2, 2) emission was detected towards all but one of the target fields and we find that their linewidths and derived temperatures are consistent with the presence of young star formation regions.

  6. The ISO spectrum of the planetary nebula NGC 6302 - I. Observations

    NARCIS (Netherlands)

    Beintema, DA; Pottasch, [No Value

    The spectrum of the planetary nebula NGC 6302 is presented, as it was observed by the ISO short-wavelength spectrometer. The IUE spectrum observed at the same position with the same aperture is also presented.

  7. Meiotic and Mitotic Phenotypes Conferred by the blm1-1 Mutation in Saccharomyces cerevisiae and MSH4 Suppression of the Bleomycin Hypersusceptibility

    Directory of Open Access Journals (Sweden)

    Carol Wood Moore


    Full Text Available Abstract: Oxidative damage can lead to a number of diseases, and can be fatal. The blm1-1 mutation of Saccharomyces cerevisiae confers hypersusceptibility to lethal effects of the oxidative, anticancer and antifungal agent, bleomycin. For the current report, additional defects conferred by the mutation in meiosis and mitosis were investigated. The viability of spores produced during meiosis by homozygous normal BLM1/BLM1, heterozygous BLM1/blm1-1, and homozygous mutant blm1-1/blm1-1 diploid strains was studied and compared. Approximately 88% of the tetrads derived from homozygous blm1-1/blm1-1 mutant diploid cells only produced one or two viable spores. In contrast, just one tetrad among all BLM1/BLM1 and BLM1/blm1-1 tetrads only produced one or two viable spores. Rather, 94% of BLM1/BLM1 tetrads and 100% of BLM1/blm1-1 tetrads produced asci with four or three viable spores. Thus, at least one copy of the BLM1 gene is essential for the production of four viable spores after meiosis. During mitotic growth, mutant blm1-1 strains grew at reduced rates and produced cells with high frequencies of unusual morphologies compared to wild-type strains. These results indicated BLM1 is also essential for normal mitotic growth. We also investigated the suppression by the MSH4 gene, a meiosis-specific MutS homolog, of the bleomycin hypersusceptibility of blm1-1 mutant cells, and the relationship of MSH4 to BLM1. We screened a genomic library, and isolated the MSH4 gene on the basis of its ability to suppress lethal effects of bleomycin in blm1-1 cells. However, genetic mapping studies indicated that BLM1 and MSH4 are not the same gene. The possibility that chromosomal nondisjunction could be the basis for the inability of blm1-1/blm1-1 mutant cells to produce four viable spores after meiosis is discussed.

  8. Clickable, Hydrophilic Ligand for fac-[MI(CO)3]+ (M = Re/99mTc) Applied in an S-Functionalized ?-MSH Peptide


    Kasten, Benjamin B.; Ma, Xiaowei; Liu, Hongguang; Hayes, Thomas R.; Barnes, Charles L.; Qi, Shibo; Cheng, Kai; Bottorff, Shalina C.; Slocumb, Winston S.; Wang, Jing; Cheng, Zhen; Benny, Paul D.


    The copper(I)-catalyzed azide?alkyne cycloaddition (CuAAC) click reaction was used to incorporate alkyne-functionalized dipicolylamine (DPA) ligands (1 and 3) for fac-[MI(CO)3]+ (M = Re/99mTc) complexation into an ?-melanocyte stimulating hormone (?-MSH) peptide analogue. A novel DPA ligand with carboxylate substitutions on the pyridyl rings (3) was designed to increase the hydrophilicity and to decrease in vivo hepatobiliary retention of fac-[99mTcI(CO)3]+ complexes used in single photon emi...

  9. Identification of Factors Interacting with hMSH2 and hMLH1 in the Fetal Liver and Investigations of how Mitochondrial Dysfunction Creates a Mutator Phenotype

    DEFF Research Database (Denmark)

    Rasmussen, Anne Karin

    demonstrated that hMSH2 interacts with a human 5’ → 3’ exonuclease 1 (hEXO1). Data presented in this thesis also support the conclusion that mitochondrial dysfunction leads to spontaneous nuclear DNA damage. We employed the yeast Saccharomyces cerevisiae as a model system to investigate a potential link...... between mitochondrial activity and genomic instability. Mitochondrial dysfunction and genetic instability are characteristic features of cancer cells. Furthermore, mitochondrial dysfunction is a key feature of aging due to accumulation of mutations in mtDNA. Our studies in a yeast model system suggest...


    Energy Technology Data Exchange (ETDEWEB)

    Sahai, R. [Jet Propulsion Laboratory, MS 183-900, California Institute of Technology, Pasadena, CA 91109 (United States); Vlemmings, W. H. T. [Department of Earth and Space Sciences, Chalmers University of Technology, Onsala Space Observatory, SE-43992 Onsala (Sweden); Huggins, P. J. [Physics Department, New York University, 4 Washington Place, New York, NY 10003 (United States); Nyman, L.-Å. [Joint ALMA Observatory (JAO), Alonso de Cordova 3107, Vitacura, Santiago de Chile (Chile); Gonidakis, I., E-mail: [CSIRO Astronomy and Space Science, Australia Telescope National Facility, Marsfield NSW 2122 (Australia)


    The Boomerang Nebula is the coldest known object in the universe, and an extreme member of the class of pre-planetary nebulae, objects which represent a short-lived transitional phase between the asymptotic giant branch and planetary nebula evolutionary stages. Previous single-dish CO (J = 1-0) observations (with a 45'' beam) showed that the high-speed outflow in this object has cooled to a temperature significantly below the temperature of the cosmic background radiation. Here we report the first observations of the Boomerang Nebula with ALMA in the CO J = 2-1 and J = 1-0 lines to resolve the structure of this ultra-cold nebula. We find a central hourglass-shaped nebula surrounded by a patchy, but roughly round, cold high-velocity outflow. We compare the ALMA data with visible-light images obtained with the Hubble Space Telescope and confirm that the limb-brightened bipolar lobes seen in these data represent hollow cavities with dense walls of molecular gas and dust producing both the molecular-emission-line and scattered-light structures seen at millimeter and visible wavelengths. The large diffuse biconical shape of the nebula seen in the visible wavelength range is likely due to preferential illumination of the cold, high-velocity outflow. We find a compact source of millimeter-wave continuum in the nebular waist—these data, together with sensitive upper limits on the radio continuum using observations with ATCA, indicate the presence of a substantial mass of very large (millimeter-sized) grains in the waist of the nebula. Another unanticipated result is the detection of CO emission regions beyond the ultra-cold region which indicate the re-warming of the cold gas, most likely due to photoelectric grain heating.


    Directory of Open Access Journals (Sweden)

    L. V. Klyuchinskaya


    Full Text Available New exact solution of the spherically-axissymmetric Eiler's equations, called as plan­etary vortex, is applied to the problem of formation in planetary nebula germs of planets due to the condensation of gases in the areas of vortex instability which calls the rings of planetary vortex. It is shown that the vortex perturbations causes changes in preassure and temperature at which the gases of nebula condense themselves, forming the germs of the planets.

  12. Wind conditions for wind turbine design

    Energy Technology Data Exchange (ETDEWEB)

    Maribo Pedersen, B.


    Delegates from Europe and USA attended the meeting and discussed general aspects of wind conditions for wind turbine design. The subjects and the presented papers covered a very broad range of aspects of wind conditions and related influence on the wind turbine. (EHS)

  13. A polymorphism in the MSH3 mismatch repair gene is associated with the levels of somatic instability of the expanded CTG repeat in the blood DNA of myotonic dystrophy type 1 patients. (United States)

    Morales, Fernando; Vásquez, Melissa; Santamaría, Carolina; Cuenca, Patricia; Corrales, Eyleen; Monckton, Darren G


    Somatic mosaicism of the expanded CTG repeat in myotonic dystrophy type 1 is age-dependent, tissue-specific and expansion-biased, contributing toward the tissue-specificity and progressive nature of the symptoms. Previously, using regression modelling of repeat instability we showed that variation in the rate of somatic expansion in blood DNA contributes toward variation in age of onset, directly implicating somatic expansion in the disease pathway. Here, we confirm these results using a larger more genetically homogenous Costa Rican DM1 cohort (pCTG repeat than other genotypes. Interestingly, this SNP results in an amino acid change in the critical ATPase domain of MSH3 and is potentially functionally dimorphic. These data suggest that MSH3 is a key player in generating somatic variation in DM1 patients and further highlight MSH3 as a potential therapeutic target. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Wind Technologies & Evolving Opportunities (Presentation)

    Energy Technology Data Exchange (ETDEWEB)

    Robichaud, R.


    This presentation covers opportunities for wind technology; wind energy market trends; an overview of the National Wind Technology Center near Boulder, Colorado; wind energy price and cost trends; wind turbine technology improvements; and wind resource characterization improvements.

  15. Turbulent Concentration of mm-Size Particles in the Protoplanetary Nebula: Scale-Dependent Cascades (United States)

    Cuzzi, J. N.; Hartlep, T.


    The initial accretion of primitive bodies (here, asteroids in particular) from freely-floating nebula particles remains problematic. Traditional growth-by-sticking models encounter a formidable "meter-size barrier" (or even a mm-to-cm-size barrier) in turbulent nebulae, making the preconditions for so-called "streaming instabilities" difficult to achieve even for so-called "lucky" particles. Even if growth by sticking could somehow breach the meter size barrier, turbulent nebulae present further obstacles through the 1-10km size range. On the other hand, nonturbulent nebulae form large asteroids too quickly to explain long spreads in formation times, or the dearth of melted asteroids. Theoretical understanding of nebula turbulence is itself in flux; recent models of MRI (magnetically-driven) turbulence favor low-or- no-turbulence environments, but purely hydrodynamic turbulence is making a comeback, with two recently discovered mechanisms generating robust turbulence which do not rely on magnetic fields at all. An important clue regarding planetesimal formation is an apparent 100km diameter peak in the pre-depletion, pre-erosion mass distribution of asteroids; scenarios leading directly from independent nebula particulates to large objects of this size, which avoid the problematic m-km size range, could be called "leapfrog" scenarios. The leapfrog scenario we have studied in detail involves formation of dense clumps of aerodynamically selected, typically mm-size particles in turbulence, which can under certain conditions shrink inexorably on 100-1000 orbit timescales and form 10-100km diameter sandpile planetesimals. There is evidence that at least the ordinary chondrite parent bodies were initially composed entirely of a homogeneous mix of such particles. Thus, while they are arcane, turbulent concentration models acting directly on chondrule size particles are worthy of deeper study. The typical sizes of planetesimals and the rate of their formation can be

  16. Wind Power Meteorology

    DEFF Research Database (Denmark)

    Lundtang Petersen, Erik; Mortensen, Niels Gylling; Landberg, Lars

    Wind power meteorology has evolved as an applied science, firmly founded on boundary-layer meteorology, but with strong links to climatology and geography. It concerns itself with three main areas: siting of wind turbines, regional wind resource assessment, and short-term prediction of the wind...... resource. The history, status and perspectives of wind power meteorology are presented, with emphasis on physical considerations and on its practical application. Following a global view of the wind resource, the elements of boundary layer meteorology which are most important for wind energy are reviewed......: wind profiles and shear, turbulence and gust, and extreme winds. The data used in wind power meteorology stem mainly from three sources: onsite wind measurements, the synoptic networks, and the re-analysis projects. Wind climate analysis, wind resource estimation and siting further require a detailed...

  17. Bioaugmentation potential of free and formulated 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in soil, sand and water. (United States)

    Schultz-Jensen, Nadja; Aamand, Jens; Sørensen, Sebastian R


    Pesticides are used extensively worldwide, which has led to the unwanted contamination of soil and water resources. Former use of the herbicide 2,6-dichlorobenzonitrile (dichlobenil) has caused pollution of ground and surface water resources by the stable degradation product 2,6-dichlorobenzamide (BAM) in several parts of Europe, which has resulted in the costly closure of several drinking water wells. One strategy for preventing this in future is bioaugmentation using bacterial degraders. BAM-degrading Aminobacter sp. MSH1 was therefore formulated into dried beads and tests undertaken to establish their potential for use in the remediation of polluted soil, sand and water. The formulation procedure included freeze drying, combined with trehalose addition for cell wall protection, thus ensuring a high amount of viable cells following prolonged storage at room temperature. The beads were round-shaped pellets with a diameter of about 1.25 mm, a dry matter content of approximately 95 % and an average viable cell content of 4.4 × 10(9) cells/g bead. Formulated MSH1 cells led to a similar, and frequently even faster, BAM mineralisation (20-65 % (14)CO2 produced from (14)C-labelled BAM) in batch tests conducted with sand, water and different soil moisture contents compared to adding free cells. Furthermore, the beads were easy to handle and had a shelf life of several months.

  18. Melanocytes expressing MC1R polymorphisms associated with red hair color have altered MSH-ligand activated pigmentary responses in coculture with keratinocytes. (United States)

    Roberts, Donald W; Newton, Richard A; Leonard, J Helen; Sturm, Richard A


    The occurrence of red hair and pale skin in individuals, which is associated with UV-radiation sensitivity and increased skin cancer risk, is mainly due to polymorphisms in the melanocortin-1 receptor (MC1R) expressed in melanocytes. We have established a serum free human melanocyte-keratinocyte coculture system to study the behavior and functional abilities of melanocytes expressing MC1R red hair color (RHC) variants in order to identify differences from their wild type (WT) counterparts. This model revealed the importance of elevated calcium levels in promoting strong melanocyte interaction with the surrounding keratinocytes and resulted in a dendritic melanocyte morphology similar to that in skin. However, the dendricity response following agonist activation of the MC1R receptor by NDP-MSH peptide, was markedly enhanced in WT melanocytes in comparison to RHC strains. Analysis of mRNA expression and protein levels of the major pigmentation markers following NDP-MSH treatment distinguished the enzyme dopachrome tautomerase as preferentially upregulated in cocultures of WT strains, with negligible or a much reduced response in melanocytes with RHC variant alleles. These results highlight the use of the coculture system in determining fundamental differences in the physiology of melanocytes expressing RHC MC1R receptors and those of WT genotype, which are likely to contribute to the increased skin cancer risk for individuals that carry these variants. (c) 2007 Wiley-Liss, Inc.

  19. Contribution of Chromosomes 1HchS and 6HchS to Fertility Restoration in the Wheat msH1 CMS System under Different Environmental Conditions.

    Directory of Open Access Journals (Sweden)

    Almudena Castillo

    Full Text Available Exploiting hybrid wheat heterosis has been long pursued to increase crop yield, stability and uniformity. Cytoplasmic male sterility (CMS systems based in the nuclear-cytoplasmic incompatible interactions are a classic way for hybrid seed production, but to date, no definitive system is available in wheat. The msH1 CMS system results from the incompatibility between the nuclear genome of wheat and the cytoplasmic genome of the wild barley Hordeum chilense. Fertility restoration of the CMS phenotype was first associated with the disomic addition of the short arm of chromosome 6H from H. chilense. In further studies it was observed that chromosome arm 1HchS was also implicated, and the combination of genes in both chromosome arms restored fertility more efficiently. In this work we aim to dissect the effect of each chromosome in fertility restoration when combined in different genomic backgrounds and under different environmental conditions. We propose a model to explain how restoration behaves in the msH1 system and generate valuable information necessary to develop an efficient system for hybrid wheat production.

  20. 2,6-Dichlorobenzamide (BAM) herbicide mineralisation by Aminobacter sp. MSH1 during starvation depends on a subpopulation of intact cells maintaining vital membrane functions. (United States)

    Sjøholm, Ole R; Nybroe, Ole; Aamand, Jens; Sørensen, Jan


    Mineralisation capability was studied in the 2,6-dichlorobenzamide (BAM)-degrading Aminobacter sp. MSH1 under growth-arrested conditions. Cells were starved in mineral salts (MS) solution or groundwater before (14)C-labelled BAM (0.1mM) was added. Cell physiology was monitored with a panel of vitality stains combined with flow cytometry to differentiate intact, depolarised and dead cells. Cells starved for up to 3 weeks in MS solution showed immediate growth-linked mineralisation after BAM amendment while a lag-phase was seen after 8 weeks of starvation. In contrast, cells amended with BAM in natural groundwater showed BAM mineralisation but no growth. The cell-specific mineralisation rate was always comparable (10(-16)molCintact cell(-1)day(-1)) independent of media, growth, or starvation period after BAM amendment; lower rates were only observed as BAM concentration decreased. MSH1 seems useful for bioremediation and should be optimised to maintain an intact cell subpopulation as this seems to be the key parameter for successful mineralisation. Copyright © 2010 Elsevier Ltd. All rights reserved.

  1. The WC6 Wolf-Rayet star MLA 1159 in M31 and its ionization nebula BA 1-642


    Greiner, J.; Tovmassian, G. H.; Komossa, S.; M. Rosado; Arrieta, A.


    We report on optical imaging and spectroscopic observations of the Wolf-Rayet candidate star MLA 1159 and the surrounding ionization nebula BA 1-642 in the Andromeda Galaxy. Though both objects have been known for many years, our observations (1) confirm the Wolf-Rayet nature of MLA 1159, (2) allow one to determine the nebula as an ionization nebula, and (3) demonstrate the association of MLA 1159 with the nebula. The supersoft X-ray source RX J0045.5+4206 whose error box encompasses the full...

  2. Prospecting for Wind (United States)

    Swapp, Andy; Schreuders, Paul; Reeve, Edward


    Many people use wind to help meet their needs. Over the years, people have been able to harness or capture the wind in many different ways. More recently, people have seen the rebirth of electricity-generating wind turbines. Thus, the age-old argument about technology being either good or bad can also be applied to the wind. The wind can be a…

  3. Careers in Wind Energy (United States)

    Liming, Drew; Hamilton, James


    As a common form of renewable energy, wind power is generating more than just electricity. It is increasingly generating jobs for workers in many different occupations. Many workers are employed on wind farms: areas where groups of wind turbines produce electricity from wind power. Wind farms are frequently located in the midwestern, western, and…

  4. Hydrodynamic Models of Line-Driven Accretion Disk Winds III: Local Ionization Equilibrium (United States)

    Pereyra, Nicolas Antonio; Kallman, Timothy R.; White, Nicholas E. (Technical Monitor)


    We present time-dependent numerical hydrodynamic models of line-driven accretion disk winds in cataclysmic variable systems and calculate wind mass-loss rates and terminal velocities. The models are 2.5-dimensional, include an energy balance condition with radiative heating and cooling processes, and includes local ionization equilibrium introducing time dependence and spatial dependence on the line radiation force parameters. The radiation field is assumed to originate in an optically thick accretion disk. Wind ion populations are calculated under the assumption that local ionization equilibrium is determined by photoionization and radiative recombination, similar to a photoionized nebula. We find a steady wind flowing from the accretion disk. Radiative heating tends to maintain the temperature in the higher density wind regions near the disk surface, rather than cooling adiabatically. For a disk luminosity L (sub disk) = solar luminosity, white dwarf mass M(sub wd) = 0.6 solar mass, and white dwarf radii R(sub wd) = 0.01 solar radius, we obtain a wind mass-loss rate of M(sub wind) = 4 x 10(exp -12) solar mass yr(exp -1) and a terminal velocity of approximately 3000 km per second. These results confirm the general velocity and density structures found in our earlier constant ionization equilibrium adiabatic CV wind models. Further we establish here 2.5D numerical models that can be extended to QSO/AGN winds where the local ionization equilibrium will play a crucial role in the overall dynamics.

  5. 75 FR 23263 - Alta Wind I, LLC; Alta Wind II, LLC; Alta Wind III, LLC; Alta Wind IV, LLC; Alta Wind V, LLC... (United States)


    ... Energy Regulatory Commission Alta Wind I, LLC; Alta Wind II, LLC; Alta Wind III, LLC; Alta Wind IV, LLC; Alta Wind V, LLC; Alta Wind VI, LLC; Alta Wind VII, LLC; Alta Wind VIII, LLC; Alta Windpower... Federal Energy Regulatory Commission (Commission), 18 CFR 285.207 (2009), Alta Wind I, LLC, Alta Wind II...

  6. Tower Winds - Cape Kennedy (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitized data taken from Wind Gust Charts. Record contains hourly wind directions and speed with a peak wind recorded at the end of each day. Sorted by: station,...


    Energy Technology Data Exchange (ETDEWEB)

    Sahai, R. [Jet Propulsion Laboratory, California Institute of Technology, MS 183-900, Pasadena, CA 91109 (United States); Patel, N. A., E-mail: [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA (United States)


    We report interferometric mapping of the bipolar pre-planetary nebula IRAS 08005-2356 (I 08005) with an angular resolution of ∼1″–5″, using the Submillimeter Array, in the {sup 12}CO J = 2–1, 3–2, {sup 13}CO J = 2–1, and SiO J = 5–4 (v = 0) lines. Single-dish observations, using the SMT 10 m, were made in these lines as well as in the CO J = 4–3 and SiO J = 6–5 (v = 0) lines. The line profiles are very broad, showing the presence of a massive (>0.1 M{sub ⊙}), extreme high velocity outflow (V ∼ 200 km s{sup −1}) directed along the nebular symmetry axis derived from the Hubble Space Telescope imaging of this object. The outflow's scalar momentum far exceeds that available from radiation pressure of the central post-AGB star, and it may be launched from an accretion disk around a main-sequence companion. We provide indirect evidence for such a disk from its previously published, broad Hα emission profile, which we propose results from Lyβ emission generated in the disk followed by Raman-scattering in the innermost regions of a fast, neutral wind.

  8. Wind Farm Power Forecasting


    Haouas, Nabiha; Bertrand, Pierre R.


    Forecasting annual wind power production is useful for the energy industry. Until recently, attention has only been paid to the mean annual wind power energy and statistical uncertainties on this forecasting. Recently, Bensoussan et al. (2012) have pointed that the annual wind power produced by one wind turbine is a Gaussian random variable under a reasonable set of assumptions. Moreover, they can derive both mean and quantiles of annual wind power produced by one wind ...

  9. Modelling Wind for Wind Farm Layout Optimization Using Joint Distribution of Wind Speed and Wind Direction


    Ju Feng; Wen Zhong Shen


    Reliable wind modelling is of crucial importance for wind farm development. The common practice of using sector-wise Weibull distributions has been found inappropriate for wind farm layout optimization. In this study, we propose a simple and easily implementable method to construct joint distributions of wind speed and wind direction, which is based on the parameters of sector-wise Weibull distributions and interpolations between direction sectors. It is applied to the wind measurement data a...

  10. Superconducting Wind Turbine Generators


    Yunying Pan; Danhzen Gu


    Wind energy is well known as a renewable energy because its clean and less polluted characteristic, which is the foundation of development modern wind electricity. To find more efficient wind turbine is the focus of scientists around the world. Compared from conventional wind turbines, superconducting wind turbine generators have advantages at zero resistance, smaller size and lighter weight. Superconducting wind turbine will inevitably become the main trends in this area. This paper intends ...

  11. Solar system-sized condensations in the Orion Nebula

    Energy Technology Data Exchange (ETDEWEB)

    Churchwell, E.; Wood, D.O.S.; Felli, M.; Massi, M


    New observations of 22 ultracompact radio sources toward the Trapezium cluster and toward the core of the Kleinmann-Low nebula are reported. The diameters of the sources range from less than 3.7 x 10 to the 14th cm to about 3.4 x 10 to the 15th cm. Their emission measures are typically greater than 10 to the 8th pc/cm exp 6, their electron densities are greater than or equal to 10 to the 6th/cu cm, and most are optically thin at 2 cm. Fifteen are associated with stars. Two interpretations for the optically visible nebular condensations are discussed. They may be dense, molecular globules embedded in the diffuse H II region with envelopes ionized by the Trapezium stars, or they may be low-mass, premain-sequence stars whose accretion disks are slowly being evaporated by the Trapezium stars. Properties and mass-loss rates are derived for the objects not associated with visible stars. 39 references.

  12. Dust and molecules in extra-galactic planetary nebulae (United States)

    Garcia-Hernandez, Domingo Aníbal


    Extra-galactic planetary nebulae (PNe) permit the study of dust and molecules in metallicity environments other than the Galaxy. Their known distances lower the number of free parameters in the observations vs. models comparison, providing strong constraints on the gas-phase and solid-state astrochemistry models. Observations of PNe in the Galaxy and other Local Group galaxies such as the Magellanic Clouds (MC) provide evidence that metallicity affects the production of dust as well as the formation of complex organic molecules and inorganic solid-state compounds in their circumstellar envelopes. In particular, the lower metallicity MC environments seem to be less favorable to dust production and the frequency of carbonaceous dust features and complex fullerene molecules is generally higher with decreasing metallicity. Here, I present an observational review of the dust and molecular content in extra-galactic PNe as compared to their higher metallicity Galactic counterparts. A special attention is given to the level of dust processing and the formation of complex organic molecules (e.g., polycyclic aromatic hydrocarbons, fullerenes, and graphene precursors) depending on metallicity.


    Energy Technology Data Exchange (ETDEWEB)

    Jacoby, George H. [Giant Magellan Telescope/Carnegie Observatories, 813 Santa Barbara Street, Pasadena, CA 91101 (United States); Ciardullo, Robin [Department of Astronomy and Astrophysics, The Pennsylvania State University, University Park, PA 16802 (United States); De Marco, Orsola [Department of Physics and Astronomy, Macquarie University, Sydney, NSW 2109 (Australia); Lee, Myung Gyoon [Astronomy Program, Department of Physics and Astronomy, Seoul National University, Seoul 151-742 (Korea, Republic of); Herrmann, Kimberly A. [Lowell Observatory, Flagstaff, AZ 86001 (United States); Hwang, Ho Seong; Davies, James E. [Smithsonian Astrophysical Observatory, 60 Garden Street, Cambridge, MA 02138 (United States); Kaplan, Evan, E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [Vassar College, 124 Raymond Ave., Poughkeepsie, NY 12604 (United States)


    We report the results of an [O III] {lambda}5007 spectroscopic survey for planetary nebulae (PNe) located within the star clusters of M31. By examining R {approx} 5000 spectra taken with the WIYN+Hydra spectrograph, we identify 3 PN candidates in a sample of 274 likely globular clusters, 2 candidates in objects which may be globular clusters, and 5 candidates in a set of 85 younger systems. The possible PNe are all faint, between {approx}2.5 and {approx}6.8 mag down the PN luminosity function, and, partly as a consequence of our selection criteria, have high excitation, with [O III] {lambda}5007 to H{beta} ratios ranging from 2 to {approx}> 12. We discuss the individual candidates, their likelihood of cluster membership, and the possibility that they were formed via binary interactions within the clusters. Our data are consistent with the suggestion that PN formation within globular clusters correlates with binary encounter frequency, though, due to the small numbers and large uncertainties in the candidate list, this study does not provide sufficient evidence to confirm the hypothesis.

  14. The dusty silhouette jet HH 1019 in the Carina Nebula (United States)

    Reiter, Megan; Kiminki, Megan M.; Smith, Nathan; Bally, John


    We report the discovery in Hubble Space Telescope (HST) images of the new Herbig-Haro jet, HH 1019, located near the Tr 14 cluster in the Carina Nebula. Like other HH jets in the region, this bipolar collimated flow emerges from the head of a dark dust pillar. However, HH 1019 is unique because - unlike all other HH jets known to date - it is identified by a linear chain of dark, dusty knots that are seen primarily in silhouette against the background screen of the H II region. Proper motions confirm that these dark condensations move along the jet axis at high speed. [S ii] emission traces a highly collimated jet that is spatially coincident with these dust knots. The high extinction in the body of the jet suggests that this outflow has lifted a large amount of dust directly from the disc, although it is possible that it has entrained dust from its surrounding protostellar envelope before exiting the dust pillar. If dust in HH 1019 originates from the circumstellar disc, this provides further evidence for a jet launched from a range of radii in the disc, including those outside the dust sublimation radius. HH 1019 may be the prototype for a new subclass of dusty HH objects seen primarily in extinction against the background screen of a bright H II region. Such jets may be common, but difficult to observe because they require the special condition of a very bright background in order to be seen in silhouette.

  15. Orbital Resonances in the Solar Nebula: Strengths and Weaknesses (United States)

    Malhotra, Renu


    A planetesimal moving in the Solar Nebula experiences an aerodynamic drag which causes its orbit to circularize and shrink. However, resonant perturbations from a protoplanet interior to the planetesimal's orbit can counteract both the orbital decay and the damping of the eccentricity: the planetesimal can be captured into an orbital resonance and its eccentricity pumped up to a modestly high equilibrium value. Thus, orbital resonances constitute (partial) barriers to the delivery of planetesimals into the feeding zone of the protoplanet. We have established the characteristics of the phenomenon of resonance capture by gas drag in the circular restricted three-body approximation. We have determined the strengths of the equilibrium resonant orbits with respect to impulsive velocity perturbations. We conclude that planetesimals captured in orbital resonances are quite vulnerable to being dislocated from these orbits by mutual planetesimal interactions, but that the resonances are effective in slowing down the rate of orbital decay of planetesimals. Only very small bodies, less than or equal to 100 m, are able to reach a approx. 1 Earth mass protoplanet without being slowed down by resonances.

  16. Understanding Galactic planetary nebulae with precise/reliable nebular abundances (United States)

    García-Hernández, D. A.; Ventura, P.; Delgado-Inglada, G.; Dell'Agli, F.; di Criscienzo, M.; Yagüe, A.


    We compare recent precise/reliable nebular abundances - as derived from high-quality optical spectra and the most recent ICFs - in a sample of Galactic planetary nebulae (PNe) with nucleosynthesis predictions (HeCNOCl) from asymptotic giant branch (AGB) ATON models in the metallicity range Z ⊙/4 3.5 M⊙) solar/supersolar metallicity AGBs that experience hot bottom burning (HBB), but other formation channels in low-mass AGBs like extra mixing, stellar rotation, binary interaction, or He pre-enrichment cannot be disregarded until more accurate C/O ratios can be obtained. Two DC PNe show the imprint of advanced CNO processing and deep second dredge-up, suggesting progenitors masses close to the limit to evolve as core collapse supernovae (above 6 M⊙). Their actual C/O ratios, if confirmed, indicate contamination from the third dredge-up, rejecting the hypothesis that the chemical composition of such high-metallicity massive AGBs is modified exclusively by HBB.

  17. Magnetic fields in Planetary Nebulae: paradigms and related MHD frontiers (United States)

    Blackman, Eric G.


    Many, if not all, post AGB stellar systems swiftly transition from a spherical to a powerful aspherical pre-planetary nebula (pPNE) outflow phase before waning into a PNe. The pPNe outflows require engine rotational energy and a mechanism to extract this energy into collimated outflows. Just radiation and rotation are insufficient but a symbiosis between rotation, differential rotation and large scale magnetic fields remains promising. Present observational evidence for magnetic fields in evolved stars is suggestive of dynamically important magnetic fields, but both theory and observation are rife with research opportunity. I discuss how magnetohydrodynamic outflows might arise in pPNe and PNe and distinguish different between approaches that address shaping vs. those that address both launch and shaping. Scenarios involving dynamos in single stars, binary driven dynamos, or accretion engines cannot be ruled out. One appealing paradigm involves accretion onto the primary post-AGB white dwarf core from a low mass companion whose decaying accretion supply rate owers first the pPNe and then the lower luminosity PNe. Determining observational signatures of different MHD engines is a work in progress. Accretion disk theory and large scale dynamos pose many of their own fundamental challenges, some of which I discuss in a broader context.

  18. Helix Nebula: sunshine and clouds on the CERN computing horizon

    CERN Document Server

    Joannah Caborn Wengler


    23 petabytes is how much data CERN recorded during 2011, and this number will rise in 2012. In order to respond to the challenge, the IT department is upping its game, amongst other things by participating in the Helix Nebula project, a public-private partnership to create a European cloud-computing platform, as announced in a recent CERN press release.   “We’re not replacing the Grid,” clarifies Bob Jones, responsible for CERN openlab who is also responsible for EC-funded projects in IT, “but looking at three complementary ways of increasing CERN’s computing capacity, so that as demand goes up we can continue to satisfy our users.” “First we are upgrading the electrical and cooling infrastructure of the computer centre in order to increase the availability of critical IT services needed for the Laboratory. This will also provide more floor space in the area called The Barn, allowing for more servers to fit in.”...

  19. Life after stellar death: Planetary Nebulae and Supernova Remnants (United States)

    Boumis, P.


    Planetary nebulae (PNe) are powerful tracers of our Galaxy's star formation history. Their study can provide insight to the late stages of stellar evolution, the nucleosynthesis in low and intermediate mass stars (1-8Mo) and the chemical evolution of galaxies. Supernova explosions belong to the most spectacular events in the Universe. Supernova remnants (SNRs), which are the consequent results of these events and come from the late stages of massive stars (>8Mo), are among the strongest radio sources observed. They have a major influence on both the properties of the interstellar medium (ISM) and the evolution of galaxies as a whole. They enrich the ISM with heavy elements, release about 1051 ergs of energy, heat the ISM, compress the magnetic field, and efficiently accelerate, by their shock waves, energetic cosmic rays observed throughout the Galaxy. I will present results of our work on PNe and SNRs, which aims to (a) discover optical SNRs in the Galaxy, (b) study their morphology and kinematics, (c) characterize their properties (such as density, shock velocity etc.) and (d) provide information on their interaction with the ISM, using the "Aristarchos" among other telescopes.

  20. Do all Planetary Nebulae result from Common Envelopes? (United States)

    De Marco, O.; Moe, M.; Herwig, F.; Politano, M.


    The common envelope interaction is responsible for evolved close binaries. Some of these binaries reside in the middle of planetary nebulae (PN). Conventional wisdom has it that only about 10% of all PN contain close binary central stars. Recent observational results, however, strongly suggest that most or even all PN are in close binary systems. Interestingly, our population synthesis calculations predict that the number of post-common envelope PN is in agreement with the total number of PN in the Galaxy. On the other hand, if all stars (single and in binaries) with mass between ˜1-8 M⊙ eject a PN, there would be 10-20 times many more PN in the galaxy than observed. This theoretical result is in agreement with the observations in suggesting that binary interactions play a functional rather than marginal role in the creation of PN. FH acknowledges funds from the U.S. Dept. of Energy, under contract W-7405-ENG-36 to Los Alamos National Laboratory. MP gratefully acknowledges NSF grant AST-0328484 to Marquette University.

  1. Wind Power Today: Federal Wind Program Highlights

    Energy Technology Data Exchange (ETDEWEB)


    Wind Power Today is an annual publication that provides an overview of the wind research conducted under the U.S. Department of Energy's Wind and Hydropower Technologies Program. The purpose of Wind Power Today is to show how DOE supports wind turbine research and deployment in hopes of furthering the advancement of wind technologies that produce clean, low-cost, reliable energy. Content objectives include: educate readers about the advantages and potential for widespread deployment of wind energy; explain the program's objectives and goals; describe the program's accomplishments in research and application; examine the barriers to widespread deployment; describe the benefits of continued research and development; facilitate technology transfer; and attract cooperative wind energy projects with industry.

  2. Wind flow through shrouded wind turbines (United States)


    THROUGH SHROUDED WIND TURBINES by Jonathan P. Scheuermann March 2017 Thesis Advisor: Muguru Chandrasekhara Second Reader: Kevin Jones THIS......CODE 13. ABSTRACT (maximum 200 words) Wall pressure distributions and cross section flow distribution on wind turbine shroud designs, determined

  3. Turbulent Concentration of MM-Size Particles in the Protoplanetary Nebula: Scaled-Dependent Multiplier Functions (United States)

    Cuzzi, Jeffrey N.; Hartlep, Thomas; Weston, B.; Estremera, Shariff Kareem


    The initial accretion of primitive bodies (asteroids and TNOs) from freely-floating nebula particles remains problematic. Here we focus on the asteroids where constituent particle (read "chondrule") sizes are observationally known; similar arguments will hold for TNOs, but the constituent particles in those regions will be smaller, or will be fluffy aggregates, and are unobserved. Traditional growth-bysticking models encounter a formidable "meter-size barrier" [1] (or even a mm-cm-size barrier [2]) in turbulent nebulae, while nonturbulent nebulae form large asteroids too quickly to explain long spreads in formation times, or the dearth of melted asteroids [3]. Even if growth by sticking could somehow breach the meter size barrier, other obstacles are encountered through the 1-10km size range [4]. Another clue regarding planetesimal formation is an apparent 100km diameter peak in the pre-depletion, pre-erosion mass distribution of asteroids [5]; scenarios leading directly from independent nebula particulates to this size, which avoid the problematic m-km size range, could be called "leapfrog" scenarios [6-8]. The leapfrog scenario we have studied in detail involves formation of dense clumps of aerodynamically selected, typically mm-size particles in turbulence, which can under certain conditions shrink inexorably on 100-1000 orbit timescales and form 10-100km diameter sandpile planetesimals. The typical sizes of planetesimals and the rate of their formation [7,8] are determined by a statistical model with properties inferred from large numerical simulations of turbulence [9]. Nebula turbulence can be described by its Reynolds number Re = L/eta sup(4/3), where L = ETA alpha sup (1/2) the largest eddy scale, H is the nebula gas vertical scale height, and a the nebula turbulent viscosity parameter, and ? is the Kolmogorov or smallest scale in turbulence (typically about 1km), with eddy turnover time t?. In the nebula, Re is far larger than any numerical simulation can

  4. Advanced structural wind engineering

    CERN Document Server

    Kareem, Ahsan


    This book serves as a textbook for advanced courses as it introduces state-of-the-art information and the latest research results on diverse problems in the structural wind engineering field. The topics include wind climates, design wind speed estimation, bluff body aerodynamics and applications, wind-induced building responses, wind, gust factor approach, wind loads on components and cladding, debris impacts, wind loading codes and standards, computational tools and computational fluid dynamics techniques, habitability to building vibrations, damping in buildings, and suppression of wind-induced vibrations. Graduate students and expert engineers will find the book especially interesting and relevant to their research and work.

  5. Wind energy bibliography

    Energy Technology Data Exchange (ETDEWEB)



    This bibliography is designed to help the reader search for information on wind energy. The bibliography is intended to help several audiences, including engineers and scientists who may be unfamiliar with a particular aspect of wind energy, university researchers who are interested in this field, manufacturers who want to learn more about specific wind topics, and librarians who provide information to their clients. Topics covered range from the history of wind energy use to advanced wind turbine design. References for wind energy economics, the wind energy resource, and environmental and institutional issues related to wind energy are also included.

  6. Using the NVO to Measure the Distance to Planetary Nebulae from Interstellar Reddening (United States)

    Larson, Kristen A.; Navarro, S. G.


    The distance to planetary nebulae near the plane of the Milky Way can be estimated from measured interstellar reddening if the average rates of reddening per unit distance along the lines of sight are known. The reddening-distance relationship in a line of sight can be determined by measuring reddening toward known stars in the field. In this way, we determine the reddening-distance relationship for stars that are in the same field as a planetary nebula, and use the relationship to estimate the distance to the planetary nebula whose reddening can be measured but whose intrinsic magnitude is unknown. We use tools of the National Virtual Observatory in this project to build a compilation of stellar spectral type from a variety of catalogs in the field of view of the planetary nebula. Spectral type is matched with intrinsic colors and magnitudes and catalog photometry for each star in the field to calculate the reddening and distance. We demonstrate the great potential of VO tools for this method, with planetary nebula distances that compare well to previous results. The use of VO tools in this method will be especially powerful when the VO has access to results of future surveys like GAIA. This research was done at the 2008 U.S. National Virtual Observatory Summer School held in Santa Fe, New Mexico on September 3-11, 2008 and sponsored by the National Science Foundation.

  7. A Study of Planetary Nebulae using the Faint Object Infrared Camera for the SOFIA Telescope (United States)

    Davis, Jessica


    A planetary nebula is formed following an intermediate-mass (1-8 solar M) star's evolution off of the main sequence; it undergoes a phase of mass loss whereby the stellar envelope is ejected and the core is converted into a white dwarf. Planetary nebulae often display complex morphologies such as waists or torii, rings, collimated jet-like outflows, and bipolar symmetry, but exactly how these features form is unclear. To study how the distribution of dust in the interstellar medium affects their morphology, we utilize the Faint Object InfraRed CAmera for the SOFIA Telescope (FORCAST) to obtain well-resolved images of four planetary nebulae--NGC 7027, NGC 6543, M2-9, and the Frosty Leo Nebula--at wavelengths where they radiate most of their energy. We retrieve mid infrared images at wavelengths ranging from 6.3 to 37.1 micron for each of our targets. IDL (Interactive Data Language) is used to perform basic analysis. We select M2-9 to investigate further; analyzing cross sections of the southern lobe reveals a slight limb brightening effect. Modeling the dust distribution within the lobes reveals that the thickness of the lobe walls is higher than anticipated, or rather than surrounding a vacuum surrounds a low density region of tenuous dust. Further analysis of this and other planetary nebulae is needed before drawing more specific conclusions.

  8. Comparative study of mutations in SNP loci of K-RAS, hMLH1 and hMSH2 genes in neoplastic intestinal polyps and colorectal cancer. (United States)

    Yan, Zhi-Hui; Cui, Li-Hong; Wang, Xiao-Hui; Li, Chao; He, Xing


    To clarify the molecular mechanism involved in pathogenesis of colorectal cancer as well as clinical significance of genetic analysis of histological samples. A total of 480 blood and tissue specimens were collected in our hospital from January 2011 to October 2012. In the observation group, there were 120 blood specimens and 120 intestinal tract tissue specimens collected from patients with neoplastic intestinal polyps. In the control group I there were 80 blood specimens and 80 intestinal tract tissue specimens collected from patients with colorectal cancer. In the control group II there were 40 blood specimens and 40 intestinal tract tissue specimens collected from healthy individuals. The gene segments were amplified using PCR and DNA gel electrophoresis along with DNA sequence analysis were employed for the detection of the following single nucleotide polymorphisms (SNPs): K-RAS codons 12 and 13; hMLH1 (human mutS homolog 1) gene missense mutation at Va1384Asp; hMSH2 (human mutS homolog 2) gene missense mutation at 2783C/A. The mutation rate of the SNP at Va1384Asp locus of the hMLH1 gene from blood and tissue specimens in the observation group showed no statistical difference from those in the control group I. The mutation rates of SNPs in codons 12 and 13 of K-RAS and at 2783C/A locus of the hMSH2 gene were significantly lower in the observation group than in the control group I (χ(2) = 15.476, 29.670, 10.811, 16.618, 33.538, 7.898, P gene was significantly higher in the observation group when compared to the control group II (χ(2) = 10.486, 4.876, P RAS and at 2783C/A locus of the hMSH2 gene did not show any statistical difference from those in the control group II. There may be important clinical significance and relevance between neoplastic intestinal polyps and colorectal cancer in terms of the mechanisms involved in the pathogenesis.

  9. Suzaku Observations of PSR B1259-63: A New Manifestation of Relativistic Pulsar Wind

    Energy Technology Data Exchange (ETDEWEB)

    Uchiyama, Yasunobu; Tanaka, Takaaki; Takahashi, Tadayuki; Mori, Koji; Nakazawa, Kazuhiro


    We observed PSR B1259-63, a young non-accreting pulsar orbiting around a Be star SS 2883, eight times with the Suzaku satellite from July to September 2007, to characterize the X-ray emission arising from the interaction between a pulsar relativistic wind and Be star outflows. The X-ray spectra showed a featureless continuum in 0.6-10 keV, modeled by a power law with a wide range of photon index 1.3-1.8. When combined with the Suzaku PIN detector which allowed spectral analysis in the hard 15-50 keV band, X-ray spectra do show a break at {approx} 5 keV in a certain epoch. Regarding the PSR B1259-63 system as a compactified pulsar wind nebula, in which e{sup {+-}} pairs are assumed to be accelerated at the inner shock front of the pulsar wind, we attribute the X-ray spectral break to the low-energy cutoff of the synchrotron radiation associated with the Lorentz factor of the relativistic pulsar wind {gamma}{sub 1} {approx} 4 x 10{sup 5}. Our result indicates that Comptonization of stellar photons by the unshocked pulsar wind will be accessible (or tightly constrained) by observations with the Fermi Gamma-ray Space Telescope during the next periastron passage. The PSR B1259-63 system allows us to probe the fundamental properties of the pulsar wind by a direct means, being complementary to the study of large-scale pulsar wind nebulae.


    NARCIS (Netherlands)



    IRAS 17423-1755 has been recognized as a new bipolar nebula during a multi-wavelength observational program of unidentified IRAS sources with far infrared colours similar to those of known planetary nebulae. B, V, R and H alpha CCD images show a clearly marked bipolar structure with a total


    Energy Technology Data Exchange (ETDEWEB)

    Yang, Yuan-Pei; Dai, Zi-Gao [School of Astronomy and Space Science, Nanjing University, Nanjing 210093 (China); Zhang, Bing, E-mail: [Department of Physics and Astronomy, University of Nevada, Las Vegas, NV 89154 (United States)


    Fast radio bursts (FRBs) are mysterious transient sources. If extragalactic, as suggested by their relative large dispersion measures, their brightness temperatures must be extremely high. Some FRB models (e.g., young pulsar model, magnetar giant flare model, or supra-massive neutron star collapse model) suggest that they may be associated with a synchrotron nebula. Here we study a synchrotron-heating process by an FRB in a self-absorbed synchrotron nebula. If the FRB frequency is below the synchrotron self-absorption frequency of the nebula, electrons in the nebula would absorb FRB photons, leading to a harder electron spectrum and enhanced self-absorbed synchrotron emission. In the meantime, the FRB flux is absorbed by the nebula electrons. We calculate the spectra of FRB-heated synchrotron nebulae, and show that the nebula spectra would show a significant hump in several decades near the self-absorption frequency. Identifying such a spectral feature would reveal an embedded FRB in a synchrotron nebula.

  12. The Herschel Planetary Nebula Survey (HerPlaNS). I. Data overview and analysis demonstration with NGC 6781

    NARCIS (Netherlands)

    Ueta, T.; Ladjal, D.; Exter, K.M.; Otsuka, M.; Szczerba, R.; Siódmiak, N.; Guerra, Aleman I.R.; van, Hoof P. A. M.; Kastner, J.H.; Montez, R.; McDonald, I.; Wittkowski, M.; Sandin, C.; Ramstedt, S.; De, Marco O.; Villaver, E.; Chu, Y.-H.; Vlemmings, W.; Izumiura, H.; Sahai, R.; Lopez, J.A.; Balick, B.; Zijlstra, A.; Tielens, A.G.G.M.; Rattray, R. E.; Behar, E.; Blackman, E. G.; Hebden, K.; Hora, J. L.; Murakawa, K.; Nordhaus, J.; Nordon, R.; Yamamura, I.


    Context. This is the first of a series of investigations into far-IR characteristics of 11 planetary nebulae (PNe) under the Herschel Space Observatory open time 1 program, Herschel Planetary Nebula Survey (HerPlaNS).Aims: Using the HerPlaNS data set, we look into the PN energetics and variations of

  13. The PN.S elliptical galaxy survey : Data reduction, planetary nebula catalog, and basic dynamics for NGC 3379

    NARCIS (Netherlands)

    Douglas, N. G.; Napolitano, N. R.; Romanowsky, A. J.; Coccato, L.; Kuijken, K.; Merrifield, M. R.; Arnaboldi, M.; Gerhard, O.; Freeman, K. C.; Merrett, H. R.; Noordermeer, E.; Capaccioli, M.


    We present results from Planetary Nebula Spectrograph (PN. S) observations of the elliptical galaxy NGC 3379 and a description of the data reduction pipeline. We detected 214 planetary nebulae, of which 191 are ascribed to NGC 3379 and 23 to the companion galaxy NGC 3384. Comparison with data from

  14. A Numerical Turbulence Model for Multiphase Flows in the Protoplanetary Nebula (United States)

    Champney, Joelle M.; Dobrovolskis, Anthony R.; Cuzzi, Jeffrey N.


    It is thought that planets form from solid particles in a flattened, rotating, 99% gaseous nebula. These grains gradually coagulate into millimeter-to-meter sized aggregates which settle toward the midplane of the nebula. It is widely believed that the resulting dense layer eventually becomes gravitationally unstable and collapses into 'planetesimals.' A new numerical model is presented to simulate the predominant processes (gravitation, vertical convection, and shear-driven turbulence) during the stage while the particulate material is still dispersed about the midplane of the nebula. In our previous work, particles were assumed to be spheres of a single radius; in the present work, particles are spheres of different radii. Results indicate that neither a broad nor a narrow distribution of particle sizes is likely to become gravitationally unstable.

  15. Periodic Light Variability in Twelve Carbon-rich Proto-Planetary Nebulae (United States)

    Hrivnak, Bruce J.; Lu, Wenxian; Maupin, Richard E.; Spitzbart, Bradley D.


    We present the results of a long-term (14 year) photometric monitoring program of 12 carbon-rich proto-planetary nebulae (PPNs). PPNs are objects in the evolutionary transition between the AGB and planetary nebula phases. These 12 have bright central stars (V = 8-14 mag) with F-G spectral types and faint nebulae (as seen with the HST). All of the objects show a periodicity in their light variations, although there is also evidence for multiple periods or small period changes. The pulsation periods range from 35 to 153 days, with the longer periods correlated with later spectral types. In fact, a tight correlation is seen between the period and effective temperature. The light variations range from 0.15 to 0.7 mag and are larger for the cooler objects.

  16. Discovery of Spatial and Spectral Structure in the X-Ray Emission from the Crab Nebula. (United States)

    Weisskopf; Hester; Tennant; Elsner; Schulz; Marshall; Karovska; Nichols; Swartz; Kolodziejczak; O'Dell


    The Chandra X-Ray Observatory observed the Crab Nebula and pulsar during orbital calibration. Zeroth-order images with the High-Energy Transmission Grating (HETG) readout by the Advanced CCD Imaging Spectrometer spectroscopy array (ACIS-S) show a striking richness of X-ray structure at a resolution comparable to that of the best ground-based visible-light observations. The HETG-ACIS-S images reveal, for the first time, an X-ray inner ring within the X-ray torus, the suggestion of a hollow-tube structure for the torus, and X-ray knots along the inner ring and (perhaps) along the inward extension of the X-ray jet. Although complicated by instrumental effects and the brightness of the Crab Nebula, the spectrometric analysis shows systematic variations of the X-ray spectrum throughout the nebula.

  17. Two-zone model for the broadband Crab nebula spectrum: microscopic interpretation

    Directory of Open Access Journals (Sweden)

    Fraschetti F.


    Full Text Available We develop a simple two-zone interpretation of the broadband baseline Crab nebula spectrum between 10−5 eV and ~ 100 TeV by using two distinct log-parabola energetic electrons distributions. We determine analytically the very-high energy photon spectrum as originated by inverse-Compton scattering of the far-infrared soft ambient photons within the nebula off a first population of electrons energized at the nebula termination shock. The broad and flat 200 GeV peak jointly observed by Fermi/LAT and MAGIC is naturally reproduced. The synchrotron radiation from a second energetic electron population explains the spectrum from the radio range up to ~ 10 keV. We infer from observations the energy dependence of the microscopic probability of remaining in proximity of the shock of the accelerating electrons.

  18. Interstellar and Solar Nebula Materials in Cometary Dust (United States)

    Messenger, Scott; Nakamura-Messenger, Keiko; Keller, Lindsay; Nguyen, Ann; Clemett, Simon


    Laboratory studies of cometary dust collected in the stratosphere and returned from comet 81P/Wild 2 by the Stardust spacecraft have revealed ancient interstellar grains and molecular cloud organic matter that record a range of astrophysical processes and the first steps of planetary formation. Presolar materials are rarer meteorites owing to high temperature processing in the solar nebula and hydrothermal alteration on their asteroidal parent bodies. The greater preservation of presolar materials in comets is attributed to their low accretion temperatures and limited planetary processing. Yet, comets also contain a large complement of high temperature materials from the inner Solar System. Owing to the limited and biased sampling of comets to date, the proportions of interstellar and Solar System materials within them remains highly uncertain. Interstellar materials are identified by coordinated isotopic, mineralogical, and chemical measurements at the scale of individual grains. Chondritic porous interplanetary dust particles (CP IDPs) that likely derive from comets are made up of 0.1 - 10 micron-sized silicates, Fe-Ni-sulfides, oxides, and other phases bound by organic material. As much as 1% of the silicates are interstellar grains that have exotic isotopic compositions imparted by nucleosynthetic processes in their parent stars. Crystalline silicates in CP IDPs dominantly have normal isotopic compositions and probably formed in the Solar System. 81P samples include isotopically normal refractory minerals that resemble Ca-Al rich inclusions and chondrules common in meteorites. The origins of sub-micron amorphous silicates in IDPs are not certain, but at least a few % of them are interstellar grains. The remainder have isotopic compositions consistent with Solar System origins and elemental compositions that are inconsistent with interstellar grain properties, thus favoring formation in the solar nebula [4]. The organic component in comets and primitive

  19. Rosette nebula globules: Seahorse giving birth to a star (United States)

    Mäkelä, M. M.; Haikala, L. K.; Gahm, G. F.


    Context. The Rosette nebula is an H II region ionized mainly by the stellar cluster NGC 2244. Elephant trunks, globules, and globulettes are seen at the interface where the H II region and the surrounding molecular shell meet. Aims: We have observed a field in the northwestern part of the Rosette nebula where we study the small globules protruding from the shell. Our aim is to measure their properties and study their star-formation history in continuation of our earlier study of the features of the region. Methods: We imaged the region in broadband near-infrared (NIR) JsHKs filters and narrowband H2 1-0 S(1), Pβ, and continuum filters using the SOFI camera at the ESO/NTT. The imaging was used to study the stellar population and surface brightness, create visual extinction maps, and locate star formation. Mid-infrared (MIR) Spitzer IRAC and WISE and optical NOT images were used to further study the star formation and the structure of the globules. The NIR and MIR observations indicate an outflow, which is confirmed with CO observations made with APEX. Results: The globules have mean number densities of 4.6 × 104 cm-3. Pβ is seen in absorption in the cores of the globules where we measure visual extinctions of 11-16 mag. The shell and the globules have bright rims in the observed bands. In the Ks band 20 to 40% of the emission is due to fluorescent emission in the 2.12 μmH2 line similar to the tiny dense globulettes we studied earlier in a nearby region. We identify several stellar NIR excess candidates and four of them are also detected in the Spitzer IRAC 8.0 μm image and studied further. We find an outflow with a cavity wall bright in the 2.124 μmH2 line and at 8.0 μm in one of the globules. The outflow originates from a Class I young stellar object (YSO) embedded deep inside the globule. An Hα image suggests the YSO drives a possible parsec-scale outflow. Despite the morphology of the globule, the outflow does not seem to run inside the dusty fingers

  20. α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK

    DEFF Research Database (Denmark)

    Møller, Cathrine Laustrup; Kjøbsted, Rasmus; Enriori, Pablo J


    The melanocortin system includes five G-protein coupled receptors (family A) defined as MC1R-MC5R, which are stimulated by endogenous agonists derived from proopiomelanocortin (POMC). The melanocortin system has been intensely studied for its central actions in body weight and energy expenditure...... regulation, which are mainly mediated by MC4R. The pituitary gland is the source of various POMC-derived hormones released to the circulation, which raises the possibility that there may be actions of the melanocortins on peripheral energy homeostasis. In this study, we examined the molecular signaling......D) family member 1 (S237 and T596), which is independent of upstream PKA and AMPK. We find no evidence to support that -MSH-stimulated glucose uptake involves TBC1D4 phosphorylation (T642 and S704) or GLUT4 translocation....

  1. Frequent LOH at hMLH1, a highly variable SNP in hMSH3, and negligible coding instability in ovarian cancer

    DEFF Research Database (Denmark)

    Arzimanoglou, I.I.; Hansen, L.L.; Chong, D.


    the mismatch DNA repair genes in ovarian cancer (OC), using a sensitive, accurate and reliable protocol we have developed. MATERIALS AND METHODS: A combination of high-resolution GeneScan software analysis and automated DNA cycle sequencing was used. RESULTS: Negligible coding MSI was observed in selected...... sequences of mismatch DNA repair genes in our series of sixty-two ovarian tumors and matched blood DNAs. Unlike MSI, loss of one hMLH1 allele was scored in almost half (47%) of the informative cases. In addition, an SNP in hMSH3/intron 5 was found to be highly variable in OC patients. CONCLUSION: 1) Coding...... mismatch DNA repair and/or critical genes....

  2. Multi-wavelength imaging of the peculiar Vela Molecular Ridge nebula BBW 192E (United States)

    Burkert, A.; Stecklum, B.; Henning, Th.; Fischer, O.


    We present the first images of the nebula BBW 192E at near- and mid-infrared wavelengths as well as a 1.3 mm continuum map. The nebula BBW 192E is associated with the IRAS point source 08513-4201 which has a luminosity of about 1400 L_sun and a strongly rising spectral energy distribution towards mid-infrared wavelengths. The infrared images show a pronounced bipolar nebula, which is offset by about 10arcsec from the known optical emission, as well as several point sources. We interpret this morphology as evidence for an inclined disk-like structure and scattered light emerging from the lobes. This is supported by our near-infrared imaging polarimetry at sub-arcsecond resolution which furthermore indicates that the central energy source is seen directly at near-infrared wavelengths. At 1.3 millimetre, we detected a cometary shaped source in the dust continuum radiation, 151arcsec x 55arcsec in size, with a total mass of 180 M_sun as well as average hydrogen column and number densities of 4.5x1022 cm-2 and 2.6x105 cm-3, respectively. This dust cloud is also responsible for the strong spatial variation of the extinction across the nebula. We discuss the physical properties of the infrared point sources and conclude that some might be young, low-mass pre-main sequence stars. The main energy source of the nebula is an embedded intermediate-mass young stellar object. These observations are among the first infrared detections of a disk-like system associated with a bipolar nebula surrounding an intermediate-mass young stellar object. Based on observations collected at the European Southern Observatory, La Silla, Chile (Proposal-IDs: 57.B-0392, 52.7-0086, 57.D-0260, 58.D-0213, and 63.I-0173)


    Energy Technology Data Exchange (ETDEWEB)

    Liebert, James [Steward Observatory, University of Arizona, Tucson, AZ 85721 (United States); Bond, Howard E.; Ciardullo, Robin [Department of Astronomy and Astrophysics, Pennsylvania State University, University Park, PA 16802 (United States); Dufour, P. [Departement de Physique, Universite de Montreal, C.P. 6128 Succ. Centre-Ville, Montreal, Quebec H3C 3J7 (Canada); Meakes, Michael G. [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218 (United States); Renzini, Alvio [Osservatorio Astronomico di Padova, Vicolo dell' Osservatorio 5, I-35122 Padova (Italy); Gianninas, A., E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [Homer L. Dodge Department of Physics and Astronomy, University of Oklahoma, 440 W. Brooks St., Norman, OK 73019 (United States)


    EGB 6 is an ancient, low-surface-brightness planetary nebula. The central star, also cataloged as PG 0950+139, is a very hot DAOZ white dwarf (WD) with an apparent M dwarf companion, unresolved from the ground but detected initially through excesses in the JHK bands. Its kinematics indicates membership in the Galactic disk population. Inside of EGB 6 is an extremely dense emission knot-completely unexpected since significant mass loss from the WD should have ceased {approx}10{sup 5} yr ago. The electron density of the compact nebula is very high (2.2 Multiplication-Sign 10{sup 6} cm{sup -3}), as indicated by collisional de-excitation of forbidden emission lines. Hubble Space Telescope imaging and grism spectroscopy are reported here. These resolve the WD and apparent dM companion-at a separation of 0.''166, or a projected 96{sub -45}{sup +204} AU at the estimated distance of 576{sub -271}{sup +1224} pc (using the V magnitude). Much to our surprise, we found that the compact emission nebula is superposed on the dM companion, far from the photoionizing radiation of the WD. Moreover, a striking mid-infrared excess has recently been reported in the Spitzer/IRAC and MIPS bands, best fit with two dust shells. The derived ratio L{sub IR}/L{sub WD} = 2.7 Multiplication-Sign 10{sup -4} is the largest yet found for any WD or planetary nucleus. The compact nebula has maintained its high density for over three decades. We discuss two possible explanations for the origin and confinement of the compact nebula, neither of which is completely satisfactory. This leaves the genesis and confinement of the compact nebula an astrophysical puzzle, yet similar examples appear in the literature.

  4. Neutron-capture Element Abundances in Magellanic Cloud Planetary Nebulae (United States)

    Mashburn, A. L.; Sterling, N. C.; Madonna, S.; Dinerstein, Harriet L.; Roederer, I. U.; Geballe, T. R.


    We present near-infrared spectra of 10 planetary nebulae (PNe) in the Large and Small Magellanic Clouds (LMC and SMC), acquired with the FIRE and GNIRS spectrometers on the 6.5 m Baade and 8.1 m Gemini South Telescopes, respectively. We detect Se and/or Kr emission lines in eight of these objects, the first detections of n-capture elements in Magellanic Cloud PNe. Our abundance analysis shows large s-process enrichments of Kr (0.6-1.3 dex) in the six PNe in which it was detected, and Se is enriched by 0.5-0.9 dex in five objects. We also estimate upper limits to Rb and Cd abundances in these objects. Our abundance results for the LMC are consistent with the hypothesis that PNe with 2-3 M ⊙ progenitors dominate the bright end of the PN luminosity function in young gas-rich galaxies. We find no significant correlations between s-process enrichments and other elemental abundances, central star temperature, or progenitor mass, though this is likely due to our small sample size. We determine S abundances from our spectra and find that [S/H] agrees with [Ar/H] to within 0.2 dex for most objects, but is lower than [O/H] by 0.2-0.4 dex in some PNe, possibly due to O enrichment via third dredge-up. Our results demonstrate that n-capture elements can be detected in PNe belonging to nearby galaxies with ground-based telescopes, allowing s-process enrichments to be studied in PN populations with well-determined distances. This paper includes data obtained with the 6.5-m Magellan Telescopes located at Las Campanas Observatory, Chile, and with the Gemini-South Telescope at Cerro Pachon, Chile.

  5. Dusty disks around central stars of planetary nebulae

    Energy Technology Data Exchange (ETDEWEB)

    Clayton, Geoffrey C. [Department of Physics and Astronomy, Louisiana State University, Baton Rouge, LA 70803 (United States); De Marco, Orsola [Department of Physics and Astronomy, Macquarie University, Sydney, NSW 2109 (Australia); Nordhaus, Jason [Center for Computational Relativity and Gravitation, and National Technical Institute for the Deaf, Rochester Institute of Technology, Rochester, NY 14623 (United States); Green, Joel [Department of Astronomy, The University of Texas, 1 University Station, C1400, Austin, TX 78712-0259 (United States); Rauch, Thomas; Werner, Klaus [Institute for Astronomy and Astrophysics, Kepler Center for Astro and Particle Physics, Eberhard Karls University, Sand 1, D-72076 Tübingen (Germany); Chu, You-Hua, E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [Department of Astronomy, University of Illinois at Urbana-Champaign, 1002 West Green Street, Urbana, IL 61801 (United States)


    Only a few percent of cool, old white dwarfs (WDs) have infrared excesses interpreted as originating in small hot disks due to the infall and destruction of single asteroids that come within the star's Roche limit. Infrared excesses at 24 μm were also found to derive from the immediate vicinity of younger, hot WDs, most of which are still central stars of planetary nebulae (CSPNe). The incidence of CSPNe with this excess is 18%. The Helix CSPN, with a 24 μm excess, has been suggested to have a disk formed from collisions of Kuiper belt-like objects (KBOs). In this paper, we have analyzed an additional sample of CSPNe to look for similar infrared excesses. These CSPNe are all members of the PG 1159 class and were chosen because their immediate progenitors are known to often have dusty environments consistent with large dusty disks. We find that, overall, PG 1159 stars do not present such disks more often than other CSPNe, although the statistics (five objects) are poor. We then consider the entire sample of CSPNe with infrared excesses and compare it to the infrared properties of old WDs, as well as cooler post-asymptotic giant branch (AGB) stars. We conclude with the suggestion that the infrared properties of CSPNe more plausibly derive from AGB-formed disks rather than disks formed via the collision of KBOs, although the latter scenario cannot be ruled out. Finally, there seems to be an association between CSPNe with a 24 μm excess and confirmed or possible binarity of the central star.

  6. Radio planetary nebulae in the Large Magellanic Cloud (United States)

    Leverenz, Howard; Filipović, Miroslav D.; Vukotić, B.; Urošević, D.; Grieve, Kevin


    We present 21 new radio-continuum detections at catalogued planetary nebula (PN) positions in the Large Magellanic Cloud (LMC) using all presently available data from the Australia Telescope Online Archive at 3, 6, 13 and 20 cm. Additionally, 11 previously detected LMC radio PNe are re-examined with seven detections confirmed and reported here. An additional three PNe from our previous surveys are also studied. The last of the 11 previous detections is now classified as a compact H II region that makes for a total sample of 31 radio PNe in the LMC. The radio-surface-brightness-to-diameter (Σ-D) relation is parametrized as Σ ∝ D-β. With the available 6 cm Σ-D data, we construct Σ-D samples from 28 LMC PNe and nine Small Magellanic Cloud (SMC) radio-detected PNe. The results of our sampled PNe in the MCs are comparable to previous measurements of the Galactic PNe. We obtain β = 2.9 ± 0.4 for the MC PNe compared to β = 3.1 ± 0.4 for the Galaxy. For a better insight into sample completeness and evolutionary features, we reconstruct the Σ-D data probability density function (PDF). The PDF analysis implies that PNe are not likely to follow linear evolutionary paths. To estimate the significance of sensitivity selection effects, we perform a Monte Carlo sensitivity simulation on the Σ-D data. The results suggest that selection effects are significant for values larger than β ˜ 2.6 and that a measured slope of β = 2.9 should correspond to a sensitivity-free value of ˜3.4.

  7. The PAH Emission Characteristics of the Reflection Nebula NGC 2023

    Energy Technology Data Exchange (ETDEWEB)

    Peeters, Els [Department of Physics and Astronomy, University of Western Ontario, London, ON N6A 3K7 (Canada); Bauschlicher, Charles W. Jr. [Entry Systems and Technology Division, Mail Stop 230-3, NASA Ames Research Center, Moffett Field, CA 94035 (United States); Allamandola, Louis J. [NASA Ames Research Center, Space Science Division, Mail Stop 245-6, Moffett Field, CA 94035 (United States); Tielens, Alexander G. G. M. [Leiden Observatory, P.O. Box 9513, 2300 RA Leiden (Netherlands); Ricca, Alessandra [Carl Sagan Center, SETI Institute, 189 N. Bernardo Avenue, Suite 100, Mountain View, CA 94043 (United States); Wolfire, Mark G., E-mail: [Astronomy Department, University of Maryland, College Park, MD 20742 (United States)


    We present 5–20 μ m spectral maps of the reflection nebula NGC 2023 obtained with the Infrared Spectrograph SL and SH modes on board the Spitzer Space Telescope, which reveal emission from polycyclic aromatic hydrocarbons (PAHs), C{sub 60}, and H{sub 2} superposed on a dust continuum. We show that several PAH emission bands correlate with each other and exhibit distinct spatial distributions that reveal a spatial sequence with distance from the illuminating star. We explore the distinct morphology of the 6.2, 7.7, and 8.6 μ m PAH bands and find that at least two spatially distinct components contribute to the 7–9 μ m PAH emission in NGC 2023. We report that the PAH features behave independently of the underlying plateaus. We present spectra of compact, oval PAHs ranging in size from C{sub 66} to C{sub 210}, determined computationally using density functional theory, and we investigate trends in the band positions and relative intensities as a function of PAH size, charge, and geometry. Based on the NASA Ames PAH database, we discuss the 7–9 μ m components in terms of band assignments and relative intensities. We assign the plateau emission to very small grains with possible contributions from PAH clusters and identify components in the 7–9 μ m emission that likely originate in these structures. Based on the assignments and the observed spatial sequence, we discuss the photochemical evolution of the interstellar PAH family as the PAHs are more and more exposed to the radiation field of the central star in the evaporative flows associated with the Photo-Dissociation Regions in NGC 2023.

  8. Molecular Clouds in the North American and Pelican Nebulae: Structures (United States)

    Zhang, Shaobo; Xu, Ye; Yang, Ji


    We present observations of a 4.25 deg2 area toward the North American and Pelican Nebulae in the J = 1-0 transitions of 12CO, 13CO, and C18O. Three molecules show different emission areas with their own distinct structures. These different density tracers reveal several dense clouds with a surface density of over 500 M ⊙ pc-2 and a mean H2 column density of 5.8, 3.4, and 11.9 × 1021 cm-2 for 12CO, 13CO, and C18O, respectively. We obtain a total mass of 5.4 × 104 M ⊙ (12CO), 2.0 × 104 M ⊙ (13CO), and 6.1 × 103 M ⊙ (C18O) in the complex. The distribution of excitation temperature shows two phases of gas: cold gas (~10 K) spreads across the whole cloud; warm gas (>20 K) outlines the edge of the cloud heated by the W80 H II region. The kinetic structure of the cloud indicates an expanding shell surrounding the ionized gas produced by the H II region. There are six discernible regions in the cloud: the Gulf of Mexico, Caribbean Islands and Sea, and Pelican's Beak, Hat, and Neck. The areas of 13CO emission range within 2-10 pc2 with mass of (1-5) × 103 M ⊙ and line width of a few km s-1. The different line properties and signs of star-forming activity indicate they are in different evolutionary stages. Four filamentary structures with complicated velocity features are detected along the dark lane in LDN 935. Furthermore, a total of 611 molecular clumps within the 13CO tracing cloud are identified using the ClumpFind algorithm. The properties of the clumps suggest that most of the clumps are gravitationally bound and at an early stage of evolution with cold and dense molecular gas.

  9. Emergency wind erosion control (United States)

    February through May is the critical time for wind erosion in Kansas, but wind erosion can happen any time when high winds occur on smooth, wide fields with low vegetation and poor soil structure. The most effective wind erosion control is to ensure a protective cover of residue or growing crop thro...

  10. Combination Testing Using a Single MSH5 Variant alongside HLA Haplotypes Improves the Sensitivity of Predicting Coeliac Disease Risk in the Polish Population.

    Directory of Open Access Journals (Sweden)

    Agnieszka Paziewska

    Full Text Available Assessment of non-HLA variants alongside standard HLA testing was previously shown to improve the identification of potential coeliac disease (CD patients. We intended to identify new genetic variants associated with CD in the Polish population that would improve CD risk prediction when used alongside HLA haplotype analysis. DNA samples of 336 CD and 264 unrelated healthy controls were used to create DNA pools for a genome wide association study (GWAS. GWAS findings were validated with individual HLA tag single nucleotide polymorphism (SNP typing of 473 patients and 714 healthy controls. Association analysis using four HLA-tagging SNPs showed that, as was found in other populations, positive predicting genotypes (HLA-DQ2.5/DQ2.5, HLA-DQ2.5/DQ2.2, and HLA-DQ2.5/DQ8 were found at higher frequencies in CD patients than in healthy control individuals in the Polish population. Both CD-associated SNPs discovered by GWAS were found in the CD susceptibility region, confirming the previously-determined association of the major histocompatibility (MHC region with CD pathogenesis. The two most significant SNPs from the GWAS were rs9272346 (HLA-dependent; localized within 1 Kb of DQA1 and rs3130484 (HLA-independent; mapped to MSH5. Specificity of CD prediction using the four HLA-tagging SNPs achieved 92.9%, but sensitivity was only 45.5%. However, when a testing combination of the HLA-tagging SNPs and the MSH5 SNP was used, specificity decreased to 80%, and sensitivity increased to 74%. This study confirmed that improvement of CD risk prediction sensitivity could be achieved by including non-HLA SNPs alongside HLA SNPs in genetic testing.

  11. Medium-resolution échelle spectroscopy of the Red Square Nebula, MWC 922 (United States)

    Wehres, N.; Ochsendorf, B. B.; Tielens, A. G. G. M.; Cox, N. L. J.; Kaper, L.; Bally, J.; Snow, T. P.


    Context. Medium-resolution échelle spectra of the Red Square Nebula surrounding the star MWC 922 are presented. The spectra have been obtained in 2010 and 2012 using the X-shooter spectrograph mounted on the Very Large Telescope (VLT) in Paranal, Chile. The spectrum covers a wavelength range between 300 nm-2.5 μm and shows that the nebula is rich in emission lines. Aims: We aim to identify the emission lines and use them as a tool to determine the physical and chemical characteristics of the nebula. The emission lines are also used to put constraints on the structure of the nebula and on the nature of the central stars. Methods: We analyzed and identified emission lines that indicated that the Red Square Nebula consists of a low density bipolar outflow, eminent in the broad emission component seen in [Fe II], as well as in P Cygni line profiles indicative of fast outflowing material. The narrow component in the [Fe II] lines is most likely formed in the photosphere of a surrounding disk. Some of the emission lines show a pronounced double peaked profile, such as Ca II, indicating an accretion disk in Keplerian rotation around the central star. [O I] emission lines are formed in the neutral atomic zone separating the ionized disk photosphere from the molecular gas in the interior of the disk, which is prominent in molecular CO emission in the near-IR. [N II] and [S II] emission clearly originates in a low density but fairly hot (7 000-10 000 K) nebular environment. H I recombination lines trace the extended nebula as well as the photosphere of the disk. Results: These findings put constraints on the evolution of the central objects in MWC 922. The Red Square shows strong similarities to the Red Rectangle Nebula, both in morphology and in its mid-IR spectroscopic characteristics. As for the Red Rectangle, the observed morphology of the nebula reflects mass-loss in a binary system. Specifically, we attribute the biconical morphology and the associated rung

  12. Photoevaporating flows from the cometary knots in the Helix nebula (NGC 7293)


    Lopez-MArtin, L.; Raga, A. C.; Mellema, G; Henney, W.; Canto, J.


    We explain the Ha emission of the cometary knots in the Helix Nebula (NGC 7293) with an analytical model that describes the emission of the head of the globules as a photoevaporated flow produced by the incident ionizing radiation of the central star.We compare these models with the Ha emission obtained from the HST archival images of the Helix Nebula. From a comparison of the Ha emission with the predictions of the analytical model we obtain a rate of ionizing photons from the central star o...

  13. Detection of C60 and C70 in a young planetary nebula. (United States)

    Cami, Jan; Bernard-Salas, Jeronimo; Peeters, Els; Malek, Sarah Elizabeth


    In recent decades, a number of molecules and diverse dust features have been identified by astronomical observations in various environments. Most of the dust that determines the physical and chemical characteristics of the interstellar medium is formed in the outflows of asymptotic giant branch stars and is further processed when these objects become planetary nebulae. We studied the environment of Tc 1, a peculiar planetary nebula whose infrared spectrum shows emission from cold and neutral C60 and C70. The two molecules amount to a few percent of the available cosmic carbon in this region. This finding indicates that if the conditions are right, fullerenes can and do form efficiently in space.

  14. Mid-Infrared Studies of the Cool Dust Distribution in Bipolar Planetary Nebulae (United States)

    Asano, Kentaro; Miyata, Takashi; Kamizuka, Takafumi; Ueta, Toshiya


    To understand mass loss history and mass loss and dust formation history of massive AGB stars, we carried out observations of three bipolar planetary nebulae (BPNe) in 30 micron bands from a ground-based telescope. All of our targets have a compact strong emission source in the mid-infrared (MIR) around the position of the central star of planetary nebula (CSPN). These detected emissions can be originated from cool dust. Our results show that the cool dust component is compactly distributed and much more massive than previous studies indicated. These findings suggest that they experienced a strong mass loss into the equatorial direction in past.

  15. Setting up of an OpenNebula-cloud for cloud computing


    Branderud, Anders


    School of Information and Communication Technology (ICT) at Royal Institute of Technology (KTH) have 16 computers that they are not using, which they bought from PDC at KTH a couple of years ago. Teachers of KTH are interested in using these computers to let their students work on a cloud computer environment. There is an open source system called OpenNebula, which is used on many universities and by many other persons setting up a cloud computer environment. OpenNebula is an distributed virt...

  16. Modelling Wind for Wind Farm Layout Optimization Using Joint Distribution of Wind Speed and Wind Direction

    DEFF Research Database (Denmark)

    Feng, Ju; Shen, Wen Zhong


    quite well in terms of the coefficient of determination R-2. Then, the best of these joint distributions is used in the layout optimization of the Horns Rev 1 wind farm and the choice of bin sizes for wind speed and wind direction is also investigated. It is found that the choice of bin size for wind......Reliable wind modelling is of crucial importance for wind farm development. The common practice of using sector-wise Weibull distributions has been found inappropriate for wind farm layout optimization. In this study, we propose a simple and easily implementable method to construct joint...... distributions of wind speed and wind direction, which is based on the parameters of sector-wise Weibull distributions and interpolations between direction sectors. It is applied to the wind measurement data at Horns Rev and three different joint distributions are obtained, which all fit the measurement data...

  17. Wind power. [electricity generation (United States)

    Savino, J. M.


    A historical background on windmill use, the nature of wind, wind conversion system technology and requirements, the economics of wind power and comparisons with alternative systems, data needs, technology development needs, and an implementation plan for wind energy are presented. Considerable progress took place during the 1950's. Most of the modern windmills feature a wind turbine electricity generator located directly at the top of their rotor towers.

  18. Wind Power Utilization Guide. (United States)


    wind causes the bladed -rotor turbine to rotate at low speed about the hori- zontal drive shaft that is always parallel to the force of the wind . The...current meter. Another form of a vertical axis wind turbine is a vertically straight- bladed wind turbine with cyclically pitched blades (see Figure 4.15...The expres- sions for the rotor torque for a Darrieus machine can be found in Reference 4.16. The Darrieus wind turbine

  19. An Icelandic wind atlas (United States)

    Nawri, Nikolai; Nína Petersen, Gudrun; Bjornsson, Halldór; Arason, Þórður; Jónasson, Kristján


    While Iceland has ample wind, its use for energy production has been limited. Electricity in Iceland is generated from renewable hydro- and geothermal source and adding wind energy has not be considered practical or even necessary. However, adding wind into the energy mix is becoming a more viable options as opportunities for new hydro or geothermal power installation become limited. In order to obtain an estimate of the wind energy potential of Iceland a wind atlas has been developed as a part of the Nordic project "Improved Forecast of Wind, Waves and Icing" (IceWind). The atlas is based on mesoscale model runs produced with the Weather Research and Forecasting (WRF) Model and high-resolution regional analyses obtained through the Wind Atlas Analysis and Application Program (WAsP). The wind atlas shows that the wind energy potential is considerable. The regions with the strongest average wind are nevertheless impractical for wind farms, due to distance from road infrastructure and power grid as well as harsh winter climate. However, even in easily accessible regions wind energy potential in Iceland, as measured by annual average power density, is among the highest in Western Europe. There is a strong seasonal cycle, with wintertime power densities throughout the island being at least a factor of two higher than during summer. Calculations show that a modest wind farm of ten medium size turbines would produce more energy throughout the year than a small hydro power plants making wind energy a viable additional option.

  20. Scientists Find X Rays from Stellar Winds That May Play Significant Role in Galactic Evolution (United States)


    Colorful star-forming regions that have captivated stargazers since the advent of the telescope 400 years ago contain gas thousands of times more energetic than previously recognized, powered by colliding stellar winds. This multimillion-degree gas radiated as X rays is one of the long-sought sources of energy and elements in the Milky Way galaxy's interstellar medium. A team led by Leisa Townsley, a senior research associate in astronomy and astrophysics at Penn State University, uncovered this wind phenomenon in the Rosette Nebula, a stellar nursery. With the Chandra X-ray Observatory, the team found that the most massive stars in the nebula produce winds that slam into each other, create violent shocks, and infuse the region with 6-million-degree gas. The findings are presented in Washington, D.C., today at a conference entitled "Two Years of Science with Chandra." "A ghostly glow of diffuse X-ray emission pervades the Rosette Nebula and perhaps many other similar star-forming regions throughout the Galaxy," said Townsley. "We now have a new view of the engine lighting the beautiful Rosette Nebula and new evidence for how the interstellar medium may be energized." Townsley and her colleagues created a striking X-ray panorama of the Rosette Molecular Cloud from four images with Chandra's Advanced CCD Imaging Spectrometer. This is a swath of the sky nearly 100 light years across sprayed with hundreds of X-ray-emitting young stars. In one corner of the Rosette Molecular Cloud lies the Rosette Nebula, called an "H II region" because the hydrogen gas there has been stripped of its electrons due to the strong ultraviolet radiation from its young stars. This region, about 5,000 light years away in the constellation Monoceros, the Unicorn, has long been a favorite among amateur astronomers. The wispy, colorful display is visible with small telescopes. The Chandra survey reveals, for the first time, 6-million-degree gas at the center of the Rosette Nebula, occupying a