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Sample records for willow salix discolor

  1. Selection of Willows (Salix sp. for Biomass Production

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    Davorin Kajba

    2014-12-01

    Full Text Available Background and Purpose: Willows compared with other species are the most suitable for biomass production in short rotations because of their very abundant growth during the first years. Nowadays, in Croatia, a large number of selected and registered willow clones are available. The main objective of the research should be to find genotypes which, with minimum nutrients, will produce the maximum quantity of biomass. Material and Methods: Clonal test of the arborescent willows include the autochthonous White Willow (Salix alba, interracial hybrids of the autochthonous White Willow and the English ‘cricket’ Willow (S. alba var. calva, interspecies hybrids (S. matsudana × S. alba, as well as multispecies hybrids of willows. Average production of dry biomass (DM∙ha-1∙a-1 per hectare was estimated in regard to the clone, survival, spacing and the number of shoots per stump. Results: The highest biomass production as well as the best adaptedness and phenotypic stability on testing site was shown by clones (‘V 374’, ‘V 461’, ‘V 578’ from 15.2 - 25.0 t∙DM∙ha-1∙a-1 originated from backcross hybrid S. matsudana × (S. matsudana × S. alba and by one S. alba clone (‘V 95’, 23.1 - 25.7 t∙DM∙ha-1∙a-1. These clones are now at the stage of registration and these results indicate significant potential for further breeding aimed at biomass production in short rotations. Conclusions: Willow clones showed high biomass production on marginal sites and dry biomass could be considerably increased with the application of intensive silvicultural and agro technical measures. No nutrition or pest control measures were applied (a practice otherwise widely used in intensive cultivation system, while weed vegetation was regulated only at the earliest age.

  2. The Study of Interactions between Active Compounds of Coffee and Willow (Salix sp. Bark Water Extract

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    Agata Durak

    2014-01-01

    Full Text Available Coffee and willow are known as valuable sources of biologically active phytochemicals such as chlorogenic acid, caffeine, and salicin. The aim of the study was to determine the interactions between the active compounds contained in water extracts from coffee and bark of willow (Salix purpurea and Salix myrsinifolia. Raw materials and their mixtures were characterized by multidirectional antioxidant activities; however, bioactive constituents interacted with each other. Synergism was observed for ability of inhibition of lipid peroxidation and reducing power, whereas compounds able to scavenge ABTS radical cation acted antagonistically. Additionally, phytochemicals from willow bark possessed hydrophilic character and thermostability which justifies their potential use as an ingredient in coffee beverages. Proposed mixtures may be used in the prophylaxis or treatment of some civilization diseases linked with oxidative stress. Most importantly, strong synergism observed for phytochemicals able to prevent lipids against oxidation may suggest protective effect for cell membrane phospholipids. Obtained results indicate that extracts from bark tested Salix genotypes as an ingredient in coffee beverages can provide health promoting benefits to the consumers; however, this issue requires further study.

  3. Songbird response to increased willow (Salix spp.) growth in Yellowstone's northern range.

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    Baril, Lisa M; Hansen, Andrew J; Renkin, Roy; Lawrence, Rick

    2011-09-01

    After nearly a century of height suppression, willows (Salix spp.) in the northern range of Yellowstone National Park, U.S.A., are increasing in height growth as a possible consequence of wolf (Canis lupus) restoration, climate change, or other factors. Regardless of the drivers, the recent release of this rare but important habitat type could have significant implications for associated songbirds that are exhibiting declines in the region. Our objective was to evaluate bird response to releasing willows by comparing willow structure and bird community composition across three willow growth conditions: height suppressed, recently released, and previously tall (i.e., tall prior to the height increase of released willows). Released and previously tall willows exhibited high and similar vertical structure, but released willows were significantly lower in horizontal structure. Suppressed willows were significantly shorter and lower in horizontal cover than released or previously tall willows. Bird richness increased along a gradient from lowest in suppressed to highest in previously tall willows, but abundance and diversity were similar between released and previously tall willows, despite lower horizontal cover in the released condition. Common Yellowthroat (Geothlypis trichas) and Lincoln's Sparrow (Melospiza lincolnii) were found in all three growth conditions; however, Yellow Warbler (Dendroica petechia), Warbling Vireo (Vireo gilvus), Willow Flycatcher (Empidonax traillii), and Song Sparrow (Melospiza melodii) were present in released and previously tall willows only. Wilson's Warbler (Wilsonia pusilla) was found in previously tall willows only, appearing to specialize on tall, dense willows. The results of our a priori habitat models indicated that foliage height diversity was the primary driver of bird richness, abundance, and diversity. These results indicate that vertical structure was a more important driver of bird community variables than horizontal

  4. A genetic linkage map of willow (Salix viminalis) based on AFLP and microsatelite markers

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    Hanley, S.; Barker, J.H.A.; Ooijen, van J.W.; Aldam, C.; Harris, S.L.; Ahman, I.; Larsson, S.; Karp, A.

    2002-01-01

    The genus Salix (willow) contains a number of species of great value as biomass crops. Efforts to breed varieties with improved biomass yields and resistances to pests and diseases are limited by the lack of knowledge on the genetic basis of the traits. We have used AFLP and microsatellite markers

  5. Feeding preferences of the willow sawfl y Nematus oligospilus (Hymenoptera: Tenthredinidae for commercial Salix clones Preferencias alimentarias de la avispa sierra de los sauces Nematus oligospilus (Hymenoptera: Tenthredinidae por clones de Salix comerciales

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    Verónica Loetti

    2012-06-01

    Full Text Available Nematus oligospilus Förster (Hymenoptera: Tenthredinidae is a willow sawfly native to the Northern Hemisphere which became a serious defoliator in willow plantations (Salix spp. of the Southern Hemisphere after being introduced in the early 1980´s. Studies on host preferences provide useful information for the development of pest management strategies where the willow sawfly may produce tree damage and economic loss. Feeding preferences of N. oligospilus larvae were evaluated in laboratory trials by simultaneously offering leaves from four willow tree clones commonly used in commercial plantations in Argentina (Salix babylonica var sacramenta Hortus, Salix nigra Marsch., S. babylonica L. x Salix alba L. 131-27 and Salix matsudana Koidz. x S. alba L. 13-44. Larvae of N. oligospilus fed on leaves from the four clones. However, insects consumed a significantly higher proportion of S. babylonica var sacramenta leaves than of leaves from the other clones. Results indicate that all clones used in the trials were palatable to the insect, and that S. babylonica var sacramenta is the preferred host for larval herbivory.Nematus oligospilus Förster (Hymenoptera: Tenthredinidae, o la avispa sierra de los sauces, es nativa del Hemisferio Norte y se ha convertido en un serio defoliador en plantaciones de sauces (Salix spp. del Hemisferio Sur, después de su introducción a principios de 1980. Los estudios sobre las preferencias de hospedador aportan información útil para el desarrollo de estrategias, donde la avispa sierra puede producir daño a los árboles y pérdidas económicas. Se evaluó la preferencia alimentaria de las larvas de N. oligospilus, mediante ensayos de laboratorio; se ofrecieron en forma simultánea hojas de cuatro clones de sauce, usados comúnmente en plantaciones comerciales en Argentina (Salix babylonica var sacramenta Hortus, Salix nigra Marsch., S. babylonica L. x Salix alba L. 131-27 and Salix matsudana Koidz. x S. alba L. 13

  6. SRC-willow (Salix viminalis) as a resource for flower-visiting insects

    Energy Technology Data Exchange (ETDEWEB)

    Reddersen, J. [National Environmental Research Institute, Ronde (Denmark). Dept. of Landscape Ecology

    2001-07-01

    The potential habitat value of commercial short rotation coppice (SRC)-willow plantations for flower-visiting insects was investigated. During 1998-2000, at a single typical intensive Danish farmland site, 11 Salix viminalis plantations were sampled by late April to quantify willow catkin abundance and flower sex. Mean plantation size was 1.1 ha and included one or more of clones: orm, rapp, ulv, jorr, christina and jorrun. Plot-year means of catkin abundance and of proportion of willows flowering were related to the coppicing cycle, i.e. the number of growth years since last harvest of plot ('year' 0-4). In 1998, the ground layer vegetation was sampled. Monitoring flower-visiting insects by means of line-transect counts failed due to the local scarcity of bees. At the plantation scale, flowering was discontinuous across the harvest cycle as it was totally absent in the year immediately following harvest. In successive years (1-4), individual willows flowered frequently and, occasionally, at high abundances, and catkin abundance increased with time. Within 3-4 year of harvest cycle, all plots flowered in most years with most plots exhibiting at least some flowering in any 1 year. Thus, willow catkin abundance was generally high in the total area due to: high frequency of flowering in plots, occasional high flowering abundance, plots not being harvested simultaneously and large total number of willows within plots and landscape. Similarly, flower sex ratio, and thus flower value, varied greatly between plots while variation was damped across plots. Alternative simultaneous flower resources in ground layer vegetation were few except for Dandelion. SRC willow may constitute an important resource for bees, even under the stress of the harvest cycle, and recommendations are given for improving this biodiversity aspect. (author)

  7. DETERMINATION OF FLAVONOIDS OF WILLOW TRIANDRA (SALIX TRIANDRA L., GROWING IN THE NORTH CAUCASUS

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    E. G. Sannikova

    2016-01-01

    Full Text Available Plants of willow genus are rich in various flavonoids. In 60-e years of the XX century the works of V. A. Kompantsev showed that leaves of willow triandra, growing in the North Caucasus, contain up to 5% of rutin. The method, which required long sample preparation (extraction of the raw material with TLC, chromatographic layer extraction with methanol, removal of the solvent, dissolution in ethanol, optical density measurements was used. Currently available and simple methods for the determination of rutin in plant material are described. Given that leaves of Salix triandra contain significant amounts of rutin and can be a potential raw material for the creation of medicines on their basis, it is essential to determine the amount of flavonoids in the leaves of Salix triandra and its branches.The purpose of this study is determination of the amount of flavonoids in the willow triandra, growing in the North Caucasus, depending on the place and time of collection.Methods. TLC and the method of differential spectrophotometry were used to study chemical reactions. Results. The presence of flavonoids in various vegetative plant organs was established using qualitative reactions (cyanidin test; boric-citric reaction; the reaction with solution of lead acetate and solution of ammonia. Rutin and quercetin flavonoids in the presence of standard samples were identified using TLC. the method of differential spectrophotometry, based on the reaction of flavonoids with aluminum chloride was used for the quantitative determination of the amount of flavonoids. It is shown that the greatest quantity of flavonoids amount accumulated in the leaves (up to 3.76%, its value in branches is slightly smaller (up to 3.26%. The branches without leaves have almost 10 times less flavonoids. It was established experimentally that the accumulation of flavonoids in raw material of Salix triandra is dependent on the place and time of

  8. De novo transcriptome and small RNA analysis of two Chinese willow cultivars reveals stress response genes in Salix matsudana.

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    Guodong Rao

    Full Text Available Salix matsudana Koidz. is a deciduous, rapidly growing, and drought resistant tree and is one of the most widely distributed and commonly cultivated willow species in China. Currently little transcriptomic and small RNAomic data are available to reveal the genes involve in the stress resistant in S. matsudana. Here, we report the RNA-seq analysis results of both transcriptome and small RNAome data using Illumina deep sequencing of shoot tips from two willow variants(Salix. matsudana and Salix matsudana Koidz. cultivar 'Tortuosa'. De novo gene assembly was used to generate the consensus transcriptome and small RNAome, which contained 106,403 unique transcripts with an average length of 944 bp and a total length of 100.45 MB, and 166 known miRNAs representing 35 miRNA families. Comparison of transcriptomes and small RNAomes combined with quantitative real-time PCR from the two Salix libraries revealed a total of 292 different expressed genes(DEGs and 36 different expressed miRNAs (DEMs. Among the DEGs and DEMs, 196 genes and 24 miRNAs were up regulated, 96 genes and 12 miRNA were down regulated in S. matsudana. Functional analysis of DEGs and miRNA targets showed that many genes were involved in stress resistance in S. matsudana. Our global gene expression profiling presents a comprehensive view of the transcriptome and small RNAome which provide valuable information and sequence resources for uncovering the stress response genes in S. matsudana. Moreover the transcriptome and small RNAome data provide a basis for future study of genetic resistance in Salix.

  9. Wastewater sludge fertilization: Biomass productivity and heavy metal bioaccumulation in two Salix species grown in southern Quebec (Canada)

    International Nuclear Information System (INIS)

    Teodorescu, T.I.; Labrecque, M.; Daigle, S.; Poisson, G.

    1993-01-01

    More than other kind of trees, fast growing tree species, such as willows, can profit from sludge application. While sludges are good fertilizers, they may also contain heavy metals which could reduce productivity and cause risks to the environment. The main aims of the present research were to study: (1) the production capacity of Salix discolor and Salix viminalis when supplied with various amounts of dried and pelleted sludge; (2) the uptake, and accumulation of heavy metals. Unrooted cuttings were planted on sandy soil in large plastic pots and grown in outdoors for 20-week period. Five doses of sludge were applied: equivalent of 200 (T1), 160 (T2), 120 (T3), 80 (T4) and 40 (T5) kg N per ha. Trees which received the largest amount of sludge showed the best growth results. The stem-branch biomass was significantly higher for Salix viminalis. The relationship between the total yield biomass Y (t/ha) and the rate of fertilization X (kg N/ha) is linear. The regression equations of prediction biomass production were established as following: Salix discolor Y = 1.807 + 0.037X and Salix viminalis Y = 2.578 + 0.042X. For both species, greatest stems-branch biomass per gram of N applied were produced by treatments 1 and 2. The amount of nitrogen per leaf area (N/LA) and per leaf dry weight (N/LW), were higher for Salix viminalis which leads us believe that its photosynthetic activity was better. The transfer coefficient did not vary between the species but was significantly different for Cd and Zn. The plants were able to absorb Cd and Zn but less of Ni, Hg and Cu and Pb. It was concluded that the dried and pelleted sludge can be a good fertilizer. The treatment is beneficial when Salix discolor and particulary Salix viminalis are used as vegetation filters for wastewater sludge purification and production purposes

  10. Transcriptomic Response of Purple Willow (Salix purpurea to Arsenic Stress

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    Aymeric Yanitch

    2017-06-01

    Full Text Available Arsenic (As is a toxic element for plants and one of the most common anthropogenic pollutants found at contaminated sites. Despite its severe effects on plant metabolism, several species can accumulate substantial amounts of arsenic and endure the associated stress. However, the genetic mechanisms involved in arsenic tolerance remains obscure in many model plant species used for land decontamination (phytoremediation, including willows. The present study assesses the potential of Salix purpurea cv. ‘Fish Creek’ for arsenic phytoextraction and reveals the genetic responses behind arsenic tolerance, phytoextraction and metabolism. Four weeks of hydroponic exposure to 0, 5, 30 and 100 mg/L revealed that plants were able to tolerate up to 5 mg/L arsenic. Concentrations of 0 and 5 mg/L of arsenic treatment were then used to compare alterations in gene expression of roots, stems and leaves using RNA sequencing. Differential gene expression revealed transcripts encoding proteins putatively involved in entry of arsenic into the roots, storage in vacuoles and potential transport through the plant as well as primary and secondary (indirect toxicity tolerance mechanisms. A major role for tannin as a compound used to relieve cellular toxicity is implicated as well as unexpected expression of the cadmium transporter CAX2, providing a potential means for internal arsenic mobility. These insights into the underpinning genetics of a successful phytoremediating species present novel opportunities for selection of dedicated arsenic tolerant crops as well as the potential to integrate such tolerances into a wider Salix ideotype alongside traits including biomass yield, biomass quality, low agricultural inputs and phytochemical production.

  11. Transcriptomic Response of Purple Willow (Salix purpurea) to Arsenic Stress

    Science.gov (United States)

    Yanitch, Aymeric; Brereton, Nicholas J. B.; Gonzalez, Emmanuel; Labrecque, Michel; Joly, Simon; Pitre, Frederic E.

    2017-01-01

    Arsenic (As) is a toxic element for plants and one of the most common anthropogenic pollutants found at contaminated sites. Despite its severe effects on plant metabolism, several species can accumulate substantial amounts of arsenic and endure the associated stress. However, the genetic mechanisms involved in arsenic tolerance remains obscure in many model plant species used for land decontamination (phytoremediation), including willows. The present study assesses the potential of Salix purpurea cv. ‘Fish Creek’ for arsenic phytoextraction and reveals the genetic responses behind arsenic tolerance, phytoextraction and metabolism. Four weeks of hydroponic exposure to 0, 5, 30 and 100 mg/L revealed that plants were able to tolerate up to 5 mg/L arsenic. Concentrations of 0 and 5 mg/L of arsenic treatment were then used to compare alterations in gene expression of roots, stems and leaves using RNA sequencing. Differential gene expression revealed transcripts encoding proteins putatively involved in entry of arsenic into the roots, storage in vacuoles and potential transport through the plant as well as primary and secondary (indirect) toxicity tolerance mechanisms. A major role for tannin as a compound used to relieve cellular toxicity is implicated as well as unexpected expression of the cadmium transporter CAX2, providing a potential means for internal arsenic mobility. These insights into the underpinning genetics of a successful phytoremediating species present novel opportunities for selection of dedicated arsenic tolerant crops as well as the potential to integrate such tolerances into a wider Salix ideotype alongside traits including biomass yield, biomass quality, low agricultural inputs and phytochemical production. PMID:28702037

  12. Phylogenetic Relationships between Four Salix L. Species Based on DArT Markers

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    Jerzy A. Przyborowski

    2013-12-01

    Full Text Available The objectives of this study were to evaluate the usefulness of DArT markers in genotypic identification of willow species and describe genetic relationships between four willow species: Salix viminalis, S. purpurea, S. alba and S. triandra. The experimental plant material comprised 53 willow genotypes of these four species, which are popularly grown in Poland. DArT markers seem to identify Salix species with a high degree of accuracy. As a result, the examined species were divided into four distinct groups which corresponded to the four analyzed species. In our study, we observed that S. triandra was very different genetically from the other species, including S. alba which is generally classified into the same subgenus of Salix. The above corroborates the findings of other authors who relied on molecular methods to reveal that the classification of S. triandra to the subgenus Salix was erroneous. The Principal Coordinate Analysis (PCoA and the neighbor-joining dendrogram also confirmed the clear division of the studied willow genotypes into four clusters corresponding to individual species. This confirmed the usefulness of DArT markers in taxonomic analyses and identification of willow species.

  13. Fungal community structure under goat willows (Salix caprea L.) growing at metal polluted site: the potential of screening in a model phytostabilisation study

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    Marjana Regvar; Matevz Likar; Andrej Piltaver; Nives Kugonic; Jane E. Smith

    2010-01-01

    Goat willow (Salix caprea L.) was selected in a previous vegetation screening study as a potential candidate for the later-stage phytostabilisation efforts at a heavily metal polluted site in Slovenia. The aims of this study were to identify the fungi colonising roots of S. caprea along the gradient of vegetation succession and...

  14. Growth of Populus and Salix Species under Compost Leachate Irrigation

    OpenAIRE

    Tooba Abedi; Shamim Moghaddami; Ebrahim Lashkar Bolouki

    2014-01-01

    According to the known broad variation in remediation capacity, three plant species were used in the experiment: two fast growing poplar’s clones - Populus deltoides, Populus euramericana, and willows Salix alba. Populus and Salix cuttings were collected from the nursery of the Populus Research Center of Safrabasteh in the eastern part of Guilan province at north of Iran. The Populus clones were chosen because of their high biomass production capacity and willow- because it is native in Iran....

  15. High yielding biomass genotypes of willow (Salix spp.) show differences in below ground biomass allocation

    International Nuclear Information System (INIS)

    Cunniff, Jennifer; Purdy, Sarah J.; Barraclough, Tim J.P.; Castle, March; Maddison, Anne L.; Jones, Laurence E.; Shield, Ian F.; Gregory, Andrew S.; Karp, Angela

    2015-01-01

    Willows (Salix spp.) grown as short rotation coppice (SRC) are viewed as a sustainable source of biomass with a positive greenhouse gas (GHG) balance due to their potential to fix and accumulate carbon (C) below ground. However, exploiting this potential has been limited by the paucity of data available on below ground biomass allocation and the extent to which it varies between genotypes. Furthermore, it is likely that allocation can be altered considerably by environment. To investigate the role of genotype and environment on allocation, four willow genotypes were grown at two replicated field sites in southeast England and west Wales, UK. Above and below ground biomass was intensively measured over two two-year rotations. Significant genotypic differences in biomass allocation were identified, with below ground allocation differing by up to 10% between genotypes. Importantly, the genotype with the highest below ground biomass also had the highest above ground yield. Furthermore, leaf area was found to be a good predictor of below ground biomass. Growth environment significantly impacted allocation; the willow genotypes grown in west Wales had up to 94% more biomass below ground by the end of the second rotation. A single investigation into fine roots showed the same pattern with double the volume of fine roots present. This greater below ground allocation may be attributed primarily to higher wind speeds, plus differences in humidity and soil characteristics. These results demonstrate that the capacity exists to breed plants with both high yields and high potential for C accumulation. - Highlights: • SRC willows are a source of biomass and act as carbon (C) sinks. • Biomass allocation was measured in 4 willow genotypes grown in two UK field sites. • The greatest yielding genotype had the greatest below ground biomass at both sites. • Below ground biomass allocation differed by up to 10% between genotypes and 94% between sites. • Environment e.g. wind

  16. Drying characteristics of willow chips and stems

    NARCIS (Netherlands)

    Gigler, J.K.; Loon, van W.K.P.; Seres, I.; Meerdink, G.; Coumans, W.J.

    2000-01-01

    In supply chains of willow (Salix viminalis) biomass to energy plants, drying is advisable in order to enable safe long-term storage, increase boiler efficiency and reduce gaseous emissions. To gain insight into the drying process, drying characteristics of willow chips and stems were investigated

  17. Expression of zinc and cadmium responsive genes in leaves of willow (Salix caprea L.) genotypes with different accumulation characteristics

    International Nuclear Information System (INIS)

    Konlechner, Cornelia; Türktaş, Mine; Langer, Ingrid; Vaculík, Marek; Wenzel, Walter W.; Puschenreiter, Markus; Hauser, Marie-Theres

    2013-01-01

    Salix caprea is well suited for phytoextraction strategies. In a previous survey we showed that genetically distinct S. caprea plants isolated from metal-polluted and unpolluted sites differed in their zinc (Zn) and cadmium (Cd) tolerance and accumulation abilities. To determine the molecular basis of this difference we examined putative homologues of genes involved in heavy metal responses and identified over 200 new candidates with a suppression subtractive hybridization (SSH) screen. Quantitative expression analyses of 20 genes in leaves revealed that some metallothioneins and cell wall modifying genes were induced irrespective of the genotype's origin and metal uptake capacity while a cysteine biosynthesis gene was expressed constitutively higher in the metallicolous genotype. The third and largest group of genes was only induced in the metallicolous genotype. These data demonstrate that naturally adapted woody non-model species can help to discover potential novel molecular mechanisms for metal accumulation and tolerance. -- Highlights: ► The transcriptional Zn/Cd response of the willow Salix caprea was quantified. ► Two genotypes from a highly contaminated and a control environment were compared. ► In addition to candidate genes an SSH library revealed over 200 metal induced genes. ► Constitutive upregulation and isolate-specific induction of genes were revealed. ► Willows adapt to environmental Zn/Cd contamination on the transcriptional level. -- Expression analyzes reveal different responses to Cd and Zn exposure of S. caprea genotypes with different heavy metal accumulation abilities

  18. Salix: A viable option for phytoremediation

    African Journals Online (AJOL)

    user

    Many animals depend on willows for food. (mostly leaf ... tolerant to deposition and resistant to moderate salinity .... mechanism may enable stabilization of toxic metals ... 'Phytoremediation of Lead, Nickel, and Copper by Salix acmophylla.

  19. New Rust Disease of Korean Willow (Salix koreensis) Caused by Melampsora yezoensis, Unrecorded Pathogen in Korea.

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    Yun, Yeo Hong; Ahn, Geum Ran; Yoon, Seong Kwon; Kim, Hoo Hyun; Son, Seung Yeol; Kim, Seong Hwan

    2016-12-01

    During the growing season of 2015, leaf specimens with yellow rust spots were collected from Salix koreensis Andersson, known as Korean willow, in riverine areas in Cheonan, Korea. The fungus on S. koreensis was identified as the rust species, Melampsora yezoensis , based on the morphology of urediniospores observed by light and scanning electron microscopy, and the molecular properties of the internal transcribed spacer rDNA region. Pathogenicity tests confirmed that the urediniospores are the causal agent of the rust symptoms on the leaves and young stems of S. koreensis . Here, we report a new rust disease of S. koreensis caused by the rust fungus, M. yezoensis , a previously unrecorded rust pathogen in Korea.

  20. Phytoextraction of risk elements by willow and poplar trees.

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    Kacálková, Lada; Tlustoš, Pavel; Száková, Jiřina

    2015-01-01

    To characterize the phytoextraction efficiency of two clones of willow trees (Salix x smithiana Willd., Salix rubens) and two clones of poplar trees (Populus nigra x maximowiczii, Populus nigra Wolterson) were planted in contaminated soil (0.4-2.0 mg Cd.kg(-1), 78-313 mg Zn.kg(-1), 21.3-118 mg Cu.kg(-1)). Field experiment was carried out in Czech Republic. The study investigated their ability to accumulate heavy metals (Cd, Zn, and Cu) in harvestable plant parts. The poplars produced higher amount of biomass than willows. Both Salix clones accumulated higher amount of Cd, Zn and Cu in their biomass (maximum 6.8 mg Cd.kg(-1), 909 mg Zn.kg(-1), and 17.7 mg Cu.kg(-1)) compared to Populus clones (maximum 2.06 mg Cd.kg(-1), 463 mg Zn.kg(-1), and 11.8 mg Cu.kg(-1)). There were no significant differences between clones of individual species. BCs for Cd and Zn were greater than 1 (the highest in willow leaves). BCs values of Cu were very low. These results indicate that Salix is more suitable plant for phytoextraction of Cd and Zn than Populus. The Cu phytoextraction potential of Salix and Populus trees was not confirmed in this experiment due to low soil availability of this element.

  1. Black Willow

    Science.gov (United States)

    R. M. Krinard

    1980-01-01

    Black willow and other species of Salix together comprise a majority of the stocking. Cottonwood is the chief associate, particularly in the early stages, but green ash, sycamore, pecan, persimmon, waterlocust, American elm, baldcypress, red maple, sugarberry, box-elder, and in some areas, silver maple are invaders preceding the next successional stage.

  2. Sex- and habitat-specific responses of a high arctic willow, Salix arctica, to experimental climate change

    Energy Technology Data Exchange (ETDEWEB)

    Jones, M.H.; Macdonald, S.E. [Univ. of Alberta, Dept. of Renewable Resources, Edmonton, AB (Canada); Henry, G.H.R. [Univ. of British Columbia, Dept. of Geography, Vancouver, BC (Canada)

    1999-10-01

    Dioecious plant species and those occupying diverse habitats may present special analytical problems to researchers examining effects of climate change. Here we report the results from two complementary studies designed to determine the importance of sex and habitat on gas exchange and growth of male and female individuals of a dioecious, circumpolar willow, Salix arctica, in the Canadian High Arctic. In field studies, male and female willows from dry and wet habitats were subjected to passively enhanced summer temperature ({approx} to 1.3 deg C) using small open-top chambers over three years. Peak season gas exchange varied significantly by willow sex and habitat. Overall net assimilation was higher in the dry habitat than in the wet, and higher in females than in males. In the dry habitat, net assimilation of females was enhanced by experimental warming, but decreased in males. In the wet habitat, net assimilation of females was substantially depressed by experimental warming, while males showed an inconsistent response. Development and growth of male and female catkins were enhanced by elevated temperature more than leaf fascicles, but leaf fascicle development and growth varied more between the two habitats, particularly in males. In a controlled environment study, male and female willows from these same wet and dry habitats were grown in a 2x2 factorial experiment including 1 x or 2 x ambient [CO{sub 2}] and 5 or 12 deg. C. The sexes responded very differently to the experimental treatments, but we found no effect of original habitat. Net assimilation in males was affected by the interaction of temperature and CO{sub 2}, but in females by CO{sub 2} only. Our results demonstrate (a) significant intraspecific and intersexual differences in arctic willow physiology and growth, (b) that these differences are affected by environmental conditions expected to accompany global climate change, and (c) that sex- and habitat-specific responses should be explicitly

  3. Phytotoxicity of polycyclic aromatic hydrocarbons to willow trees

    DEFF Research Database (Denmark)

    Thygesen, R.S.; Trapp, Stefan

    2002-01-01

    The toxicity of PAH to willow trees (Salix alba, S. viminalis, S. viminalisx;schwerinii) was investigated. Willow cuttings were grown in PAH-saturated hydroponic solution (naphthalene NAP, phenanthrene PHEN and benzo(a)pyrene BaP). Toxicity was related to aqueous solubility and was highest for NAP...

  4. Response of Salix alba L. to heavy metals and diesel fuel ...

    African Journals Online (AJOL)

    Response of Salix alba L. to heavy metals and diesel fuel contamination. ... Ni and Pb led to reduced accumulation of Pb. Presence of 5 g/kg of diesel fuel in soil significantly increased toxic influence of applied heavy metals by further reducing ... Key words: Cd, Ni, Pb, phytoremediation, phytoextraction, willow, Salix alba.

  5. Phytoremediation prospects of willow stands on contaminated sediment : A field trial

    NARCIS (Netherlands)

    Vervaeke, P; Luyssaert, S.; Mertens, J.; Meers, E.; Tack, F. M.G.; Lust, N

    2003-01-01

    Establishing fast growing willow stands on land disposed contaminated dredged sediment can result in the revaluation of this material and opens possibilities for phytoremediation. A field trial was designed to assess the impact of planting a willow stand (Salix viminalis L. 'Orm') on the dissipation

  6. The potential of willow for remediation of heavy metal polluted calcareous urban soils

    DEFF Research Database (Denmark)

    Jensen, J K; Holm, P E; Nejrup, J

    2009-01-01

    Growth performance and heavy metal uptake by willow (Salix viminalis) from strongly and moderately polluted calcareous soils were investigated in field and growth chamber trials to assess the suitability of willow for phytoremediation. Field uptakes were 2-10 times higher than growth chamber...

  7. On the irrigation requirements of cottonwood (Populus fremontii and Populus deltoides var. wislizenii) and willow (Salix gooddingii) grown in a desert environment

    Science.gov (United States)

    Hartwell, S.; Morino, K.; Nagler, P.L.; Glenn, E.P.

    2010-01-01

    Native tree plots have been established in river irrigation districts in the western U.S. to provide habitat for threatened and endangered birds. Information is needed on the effective irrigation requirements of the target species. Cottonwood (Populus spp.) and willow (Salix gooddingii) trees were grown for seven years in an outdoor plot in a desert environment in Tucson, Arizona. Plants were allowed to achieve a nearly complete canopy cover over the first four years, then were subjected to three daily summer irrigation schedules of 6.20??mm??d-1; 8.26??mm??d-1 and 15.7??mm??d-1. The lowest irrigation rate was sufficient to maintain growth and high leaf area index for cottonwoods over three years, while willows suffered considerable die-back on this rate in years six and seven. These irrigation rates were applied April 15-September 15, but only 0.88??mm??d-1 was applied during the dormant period of the year. Expressed as a fraction of reference crop evapotranspiration (ETo), recommended annual water applications plus precipitation (and including some deep drainage) were 0.83 ETo for cottonwood and 1.01 ETo for willow. Current practices tend to over-irrigate restoration plots, and this study can provide guidelines for more efficient water use. ?? 2010 Elsevier Ltd.

  8. Interaction in Short rotation coppice willow, Salix viminalis genotype mixtures

    Energy Technology Data Exchange (ETDEWEB)

    Begley, D. [Department of Biological Sciences, Queen' s University Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland (United Kingdom); McCracken, A.R. [Applied Plant Science and Biometrics Division, Agri-Food and Biosciences Institute, 18A Newforge Lane, Belfast BT9 5PX, Northern Ireland (United Kingdom)]|[Northern Ireland Horticulture and Plant Breeding Station, Applied Plant Science and Biometrics Division, Agri-Food and Biosciences Institute, Loughgall, Co., Armagh BT61 8JB, Northern Ireland (United Kingdom); Dawson, W.M. [Northern Ireland Horticulture and Plant Breeding Station, Applied Plant Science and Biometrics Division, Agri-Food and Biosciences Institute, Loughgall, Co., Armagh BT61 8JB, Northern Ireland (United Kingdom); Watson, S. [Applied Plant Science and Biometrics Division, Agri-Food and Biosciences Institute, 18A Newforge Lane, Belfast BT9 5PX, Northern Ireland (United Kingdom)

    2009-02-15

    Seven straight Salix viminalis genotypes with different levels of rust (Melampsora epitea) susceptibility were grown as mono-plots as well as being incorporated into a range of mixtures. Two 3-year harvests (2003 and 2006) were taken. Disease progress was followed on each of the individual genotypes throughout each growing season 2001-2006. In 2003 there were small but significant yield increases from the mixtures compared to the yield of individual components grown as mono-plots. These differences were not evident at the second harvest in 2006. No consistent effect of mixtures on reducing rust on the most susceptible genotype, Salix viminalis '77082' were observed. In some years, e.g. 2003, at certain times during the growing season, significant reductions were observed on Salix viminalis '77082' in certain 3-way mixtures compared to mono-plots. These, however, were not repeated consistently in subsequent years. It was concluded that the current commercial practice in many parts of Europe of planting mixtures as a disease control strategy will only be effective if there is sufficient genetic diversity between the Salix genotypes incorporated into the mixture. (author)

  9. Selection of ectomycorrhizal willow genotype in phytoextraction of heavy metals.

    Science.gov (United States)

    Hrynkiewicz, Katarzyna; Baum, Christel

    2013-01-01

    Willow clones are used for the phytoextraction of heavy metals from contaminated soils and are usually mycorrhizal. The receptiveness of willow clones for mycorrhizal inoculum varies specific to genotype; however, it is unknown if this might have a significant impact on their efficiency in phytoextraction of heavy metals. Therefore, a model system with mycorrhizal and non-mycorrhizal willows of two different genotypes--one with usually stronger natural mycorrhizal colonization (Salix dasyclados), and one with lower natural mycorrhizal colonization (S. viminalis)--was investigated for its efficiency of phytoextraction of heavy metals (Cd, Pb, Cu, Zn) from contaminated soil. Inoculation with the ectomycorrhizal fungus Amanita muscaria significantly decreased the biomass of leaves of both inoculated willow clones, and increased or had no effect on the biomass of trunks and roots of S. dasyclados and S. viminalis, respectively. The concentrations of heavy metals in the biomass of S. dasyclados were in general higher than in S. viminalis irrespective of inoculation with the ectomycorrhizal fungus. Inoculation with A. muscaria significantly decreased the concentration of Cu in the trunks of both Salix taxa, but did not affected the concentrations of other heavy metals in the biomass. In conclusion, stronger receptiveness of willow clones for mycorrhizal inoculum was correlated with an increased total extraction of heavy metals from contaminated soils. Therefore, this seems to be a suitable criterion for effective willow clone selection for phytoremediation. Increased biomass production with relatively constant metal concentrations seems to be a major advantage of mycorrhizal formation of willows in phytoremediation of contaminated soils.

  10. Preliminary tests for Salix sp. tolerance to heavy metals (Cd, Ni, Pb)

    Science.gov (United States)

    Corneanu, Mihaela; Hernea, Cornelia; Butnariu, Monica; Corneanu, Gabriel; Sărac, Ioan; Hollerbach, Wilhelm; Neţoiu, Constantin; Petcov, Andreea Adriana

    2014-05-01

    The species of Salix genus constitute potential sources of germplasm in the prevention of the environment degradation, and offer remedy for about two third from the all degradation types. The majority of the willow species, present a good adaptation to hypoxic conditions, feature which suggest that they manifest a preference for mineral nutrition in comparison with organic one. Thus, many of willow species can be developed on soils with a big amount of minerals and/or radionuclides, being both phytoremediatory species, as well as pioneer ones, contributing to the restoration of soil. Thus, the willow species, posses the capacity for development in degraded areas, natural or anthropic, as swamps, abandoning crops areas, sandy dune, riparian sandy areas, gravels, a.o. In this paper are present some laboratory comparative tests of heavy metals tolerance on four Salix sp. genotypes: clone 202 (Salix alba), hybrid 892 (Salix alba), Inger (Salix viminalis) and Gudrun (Salix viminalis). The genotypes of Salix alba are native from Romania, produced in the Forest Research and Management Institute Bucharest, while the genotypes of Salix viminalis are native from Sweden, but the plant material was produced under license in Romania by REBINA Agrar SRL. As plant material were used one-year-old cuttings (5-10 cm long), with 2-6 buds each. Per genotype, per heavy metal and each of three concentration five replication were used. There were ten experimental variants for each genotype: three concentrations for each of the heavy metals; concentrations selected, in the accordance SR ISO 11269-2/March 1999 and Control (tap water). The cuttings were maintained in metalic solutions for 15 days. In the days 7th and 15th, were performed biometrical observations on: the roots number and length, the shoots number and length, the leaves number/shoot, viability of the shoots. At the end of the experiment the content in heavy metals was determined in roots and shoots (by atomic absorption

  11. Integrated long-term responses of an arctic-alpine willow and associated ectomycorrhizal fungi to an altered environment

    DEFF Research Database (Denmark)

    Clemmensen, Karina Engelbrecht; Michelsen, Anders

    2006-01-01

    We evaluated ectomycorrhizal (ECM) colonization and morphotype community composition together with growth response and biomass distribution in the arctic-alpine, prostrate willow Salix herbacea L. x Salix polaris Wahlenb. after 11 seasons of shading, warming, and fertilization at a fellfield...

  12. Phytoextraction of heavy metals by willows growing in biosolids under field conditions.

    Science.gov (United States)

    Laidlaw, W S; Arndt, S K; Huynh, T T; Gregory, D; Baker, A J M

    2012-01-01

    Biosolids produced by sewage treatment facilities can exceed guideline thresholds for contaminant elements. Phytoextraction is one technique with the potential to reduce these elements allowing reuse of the biosolids as a soil amendment. In this field trial, cuttings of seven species/cultivars of Salix(willows) were planted directly into soil and into biosolids to identify their suitability for decontaminating biosolids. Trees were irrigated and harvested each year for three consecutive years. Harvested biomass was weighed and analyzed for the contaminant elements: As, Cd, Cu, Cr, Hg, Pb, Ni, and Zn. All Salix cultivars, except S. chilensis, growing in soils produced 10 to 20 t ha(-1) of biomass, whereas most Salix cultivars growing in biosolids produced significantly less biomass (metals from biosolids, driven by superior biomass increases and not high tissue concentrations. The willows were effectual in extracting the most soluble/exchangeable metals (Cd, 0.18; Ni, 0.40; and Zn, 11.66 kg ha(-1)), whereas Cr and Cu were extracted to a lesser degree (0.02 and 0.11 kg ha(-1)). Low bioavailable elements, As, Hg, and Pb, were not detectable in any of the aboveground biomass of the willows. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

  13. Initial spacing of poplars and willows grown as arable coppice

    Energy Technology Data Exchange (ETDEWEB)

    Armstrong, A.; Johns, C.

    1997-11-01

    Two clones of poplar and two clones of willow were grown at two sites, on a three year cutting cycle, at six different square spacings, between 0.8 metres and 1.5 metres. The two willow clones 'Bowles hybrid' and 'Dasyclados' were planted at both sites. The poplar clones Populus interamericana 'Beaupre' and Populus trichocarpa 'Columbia River'' were planted at Wishanger in Hampshire. The poplar clones Populus interamericana 'Boelare' and Populus trichocarpa 'Trichobel' were planted at Downham Market in Norfolk. The highest yield of 17.55 oven dry tonnes per hectare (odt/ha/annum) was obtained from 'Bowles hybrid', at the closest spacing, grown on a water meadow adjacent to the River Wey at Wishanger. The highest yield for all clones at both sites was achieved at the closest spacing (in this first rotation). There was a significant linear effect. One of the most interesting observations was that when comparing the gradient of the linear relationship, within species, the gradient was steeper for the higher yielding clone. This was particularly so for the willows. This would suggest that higher yielding clones are more tolerant of crowding, or, that upright Salix viminalis make better use of close space than the more spreading Salix dasyclados. The new Salix x Salix schwerinnii hybrids should therefore also be responsive to closer spacing. The same effect was observed for the poplars at Wishanger only, but the difference was not as dramatic. There was a suggestion from the highest yielding poplar plots that optimum yield may still be obtained at the currently recommended spacing of 1.0 metre x 1.0 metre. (author)

  14. Yield of tannin willows on ravine areas in the Central Forest Steppe

    Energy Technology Data Exchange (ETDEWEB)

    Sidorov, A I

    1981-01-01

    A note on the performance of 12 species of willow grown in a plantation on leached chernozem soils, to provide bark rich in tannins. The plantation was established by cuttings (25 cm long) at a spacing of 2.5 x 0.5 metres for the tree willows and 2.5 x 0.75 metres for the bush willows. Bark yields were determined at 7-8 years. The greatest yields were obtained from Salix smithiana, S. stipularis and S. acuminata: 12.0, 10.8 and 10.2 t/hectare respectively. Data on tannin yields are tabulated. Fellings for bark harvesting can be made every 5 years in plantations of bush willows and every 7 years in plantations of tree willows. The plantations can be used for 30 year with bush willows and for 50 years with tree willows.

  15. White willow sexual regeneration capacity under estuarine conditions in times of climate change

    Science.gov (United States)

    Markus-Michalczyk, Heike; Hanelt, Dieter; Denstorf, Julian; Jensen, Kai

    2016-10-01

    Tidal wetlands provide both habitats for coastal populations and wildlife, and ecosystem services for human welfare. Building with nature regarding cost-effective coastal protection is of increasing interest. Much research has been carried out on plant reproduction capacities in mangroves and salt marshes, but less is known on this issue in tidal freshwater wetlands. Willows are being successfully used for bank stabilization in riverine habitats, however, today white willow softwood forests in tidal wetlands are highly fragmented, and restoration is required e.g. by the European Habitats Directive. Recently, tolerance to increasing salinity and tidal flooding was found for vegetative propagules of floodplain willows. However, the establishment of autochthonous sexual recruits is necessary to conserve the genetic diversity of local populations, and thus may be preferable in restoration. The germination and early seedling establishment of Salix alba (white willow) was experimentally studied under simulated estuarine conditions. The species tolerance to increasing salinity (0, 0.5, 1, 1.5, and 2) was tested in a climate chamber, and its tolerance to flooding at different tidal treatments (control, spring tide, daily tide 15 min and 2 h flooding) in the greenhouse. Germination was neither affected by increasing salinity nor by tidal flooding. Salix seedlings established up to salinity 1.5, but cotyledon performance and radicle growth was largely reduced at salinity 2. Under tidal flooding, seedling growth was similar in all treatments. However, in the treatments with daily tides seedling anchorage in the substrate took more than two weeks, and fewer seedlings reached a suitable length to approach the high water line. We assess S. alba sexual regeneration under estuarine conditions as generally possible. Further studies are needed on the effects of sedimentation-erosion processes on willow establishment in the field, especially on feedbacks between Salix survival and

  16. Wood biomass: The potential of willow

    International Nuclear Information System (INIS)

    White, E.H.; Abrahamson, L.P.

    1991-10-01

    Experiments were established in central New York State in spring, 1987, to evaluate the potential of Salix for wood biomass production using ultrashort-rotation intensive-culture techniques. Five selected willow clones and one hybrid poplar clone planted at 1 x 1 foot spacing were tested for biomass production with annual coppicing. This report presents results of this research as of December 31, 1990. (VC)

  17. PHYTOREMEDIATION OF CHLORPYRIFOS BY POPULUS AND SALIX

    OpenAIRE

    Young Lee, Keum; Strand, Stuart E.; Doty, Sharon L.

    2012-01-01

    Chlorpyrifos is one of the commonly used organophosphorus insecticides that are implicated in serious environmental and human health problems. To evaluate plant potential for uptake of chlorpyrifos, several plant species of poplar (Populus sp.) and willow (Salix sp.) were investigated. Chlorpyrifos was taken up from nutrient solution by all seven plant species. Significant amounts of chlorpyrifos accumulated in plant tissues, and roots accumulated higher concentrations of chlorpyrifos than di...

  18. SELECTIVE FORAGING ON WOODY SPECIES BY THE BEAVER CASTOR FIBER, AND ITS IMPACT ON A RIPARIAN WILLOW FOREST

    NARCIS (Netherlands)

    NOLET, BA; HOEKSTRA, A; OTTENHEIM, MM

    1994-01-01

    Beavers were re-introduced in the Biesbosch, The Netherlands, a wood dominated by willows Salix spp. Conservationists expected that herbivory by beavers would enhance succession to a mixed broad-leaved forest. Willows formed the staple food of the beavers, but they removed only 1.4% of the standing

  19. Conservation assessment for the autumn willow in the Black Hills National Forest, South Dakota and Wyoming

    Science.gov (United States)

    J. Hope Hornbeck; Carolyn Hull Sieg; Deanna J. Reyher

    2003-01-01

    Autumn willow, Salix serissima (Bailey) Fern., is an obligate wetland shrub that occurs in fens and bogs in the northeastern United States and eastern Canada. Disjunct populations of autumn willow occur in the Black Hills of South Dakota. Only two populations occur on Black Hills National Forest lands: a large population at McIntosh Fen and a small...

  20. Technique for compressed bundles for harvest of whole straw willow. Pilot study; Teknik foer komprimerande helskottsskoerd av salix i buntform. Foerstudie

    Energy Technology Data Exchange (ETDEWEB)

    Eriksson, Hans (Vaesteraeng Lantbruk AB (Sweden))

    2008-06-15

    and demand good and secure weather conditions. Heavy rain or snow is a big problem during harvest season. The proposal from Maelarenergi AB has been carefully analysed and they think this is a solution with high potential. It should mean lower costs than in compare to using a mobile fossil driven cutter. Chipped Salix on farmland is a 'hot' flowchart, with big demand on among other things the logistic in and around the system. The possibility to direct consumption with chipped Salix has a limited storage capability. Chipped Salix is not possible to transport long distance, maximum 30 - 35 km, if it should keep the capacity to pay the procedure. The costs of transport will be too big in comparison to production value. Another advantage is the costs for intermediate storage. For example the area of the intermediate storage station, can be reduced when smaller volumes with higher density are handled. An overall analysis over the project 'Salix Maelardalen' point out very obviously that an alternative to the today direct chipping system is necessary and that development work around harvest with compressing bundle technique is very important. Big positive effects on environment can also be shown. Questions around 'compressed whole straw harvest of Willow' can be answered by a pilot study. There are o lot of aspects to pay attention to and 'smooth the road' to the growing production of Salix and it's rising as fuel raw material. The pilot study is going to penetrate the development of new harvest technique and earlier systems for 'compressed whole shoot harvest' of Salix. Needs and potential of new technique will be analysed and the study will also give suggestions to a new specification of requirements. The study will also suggest future developing projects. One of the purposes with the pilot project is that it could be a platform for future technical and practical research and even to be as a base for coming

  1. Hydroponic screening for metal resistance and accumulation of cadmium and zinc in twenty clones of willows and poplars

    International Nuclear Information System (INIS)

    Santos Utmazian, Maria Noel dos; Wieshammer, Gerlinde; Vega, Rosa; Wenzel, Walter W.

    2007-01-01

    We screened 20 different clones of willow and poplar species in hydroponic experiments for their metal resistance and accumulation properties. Plants were exposed for 4 weeks either to single additions of (μM) 4.45 Cd or 76.5 Zn, or a metal cocktail containing the same amounts of Cd and Zn along with 7.87 Cu and 24.1 Pb. Plant biomass, metal tolerance and metal accumulation pattern in roots and leaves varied greatly between clones. The leaf:root ratio of metal concentrations was clearly underestimated compared to soil experiments. The largest metal concentrations in leaves were detected in Salix dasyclados (315 mg Cd kg -1 d.m.) and a Salix smithiana clone (3180 mg Zn kg -1 d.m.) but these species showed low metal tolerance. In spite of smaller Cd and Zn concentrations, the metal-tolerant clones Salix matsudana, Salix fragilis-1, and Salix purpurea-1 hold promise for phytoextraction as they produced large biomass and metal contents in leaves. - Hydroponically grown willows and poplar clones accumulate up to (mg kg -1 d.w.) 315 Cd and 3180 Zn in leaves

  2. Hydroponic screening for metal resistance and accumulation of cadmium and zinc in twenty clones of willows and poplars

    Energy Technology Data Exchange (ETDEWEB)

    Santos Utmazian, Maria Noel dos [Department of Forest- and Soil Sciences, University of Natural Resources and Applied Life Sciences, Vienna, Peter-Jordan-Strasse 82, A-1190 Vienna (Austria); Wieshammer, Gerlinde [Department of Forest- and Soil Sciences, University of Natural Resources and Applied Life Sciences, Vienna, Peter-Jordan-Strasse 82, A-1190 Vienna (Austria); Vega, Rosa [Department of Forest- and Soil Sciences, University of Natural Resources and Applied Life Sciences, Vienna, Peter-Jordan-Strasse 82, A-1190 Vienna (Austria); Wenzel, Walter W. [Department of Forest- and Soil Sciences, University of Natural Resources and Applied Life Sciences, Vienna, Peter-Jordan-Strasse 82, A-1190 Vienna (Austria)]. E-mail: walter.wenzel@boku.ac.at

    2007-07-15

    We screened 20 different clones of willow and poplar species in hydroponic experiments for their metal resistance and accumulation properties. Plants were exposed for 4 weeks either to single additions of ({mu}M) 4.45 Cd or 76.5 Zn, or a metal cocktail containing the same amounts of Cd and Zn along with 7.87 Cu and 24.1 Pb. Plant biomass, metal tolerance and metal accumulation pattern in roots and leaves varied greatly between clones. The leaf:root ratio of metal concentrations was clearly underestimated compared to soil experiments. The largest metal concentrations in leaves were detected in Salix dasyclados (315 mg Cd kg{sup -1} d.m.) and a Salix smithiana clone (3180 mg Zn kg{sup -1} d.m.) but these species showed low metal tolerance. In spite of smaller Cd and Zn concentrations, the metal-tolerant clones Salix matsudana, Salix fragilis-1, and Salix purpurea-1 hold promise for phytoextraction as they produced large biomass and metal contents in leaves. - Hydroponically grown willows and poplar clones accumulate up to (mg kg{sup -1} d.w.) 315 Cd and 3180 Zn in leaves.

  3. Short-rotation cultivation of fast-growing willows and plantations of Betula pendula, Roth on Hirvineva, a mire formerly used for peat production, in Liminka, Finland. Nopeakasvuisten pajujen (Salix spp. ) lyhytkiertoviljelystae ja rauduskoivun (Betula pendula) viljelystae turvetuotannosta poistuneella suolla, limingan Hirvinevalla

    Energy Technology Data Exchange (ETDEWEB)

    Lumme, I.; Kiukaanniemi, E.

    1987-01-01

    This report is concerned with afforestation experiments using fast-growing willows (Salix spp.) and silver birch (Betula pendula, Roth) conducted by the Northern Finland Research Institute of the University of Oulu at the site of a mire formerly used for peat production, Hirvineva in the commune of Liminka (62 deg C 45{sup '} N, 23 deg C 30{sup '} E). Experiments were carried out at the early stages of willow cultivation into the use of chemical fertilizers and peat ash, the liming requirement of the soil and the effects of tilling of the soil on growth. Since 1985 efforts have been concentrated on developing new willow clones. Research was also begun into the mycorhiza which develop when willows are cultivated on peat soils. The experiments with Betula pendula were devoted to examining the effects of plantation density and fertilization with peat ash on four-year old saplings.

  4. Establishment and Early Growth of Willow at Different Levels of Weed Competition and Nitrogen Fertilization

    DEFF Research Database (Denmark)

    Edelfeldt, Stina; Lundkvist, Anneli; Forkman, Johannes

    2016-01-01

    To evaluate the effects of weed competition and nitrogen fertilization on the early growth performance of willow, cuttings of the clone Tora (Salix schwerinii x S. viminalis) were planted in buckets together with model weeds (spring barley or white mustard) sown 15, 26, and 30 days after willow...... gave higher willow biomass production in willow with weeds sown after 26 or 30 days. Type of model weed had no effect on willow performance. Weed biomass and maximum shoot height were higher in weeds planted without willows compared to the willow-weed mixtures. A high nitrogen level gave more weed...... biomass when planted without willows and in the willow-weed mixture with weeds sown after 15 days. We conclude that for the given high density of willow, competition from weeds emerging soon after willow planting had strong effect on early production. Furthermore, if there is a risk of weed infestation...

  5. Sulfur-aided phytoextraction of Cd and Zn by Salix smithiana combined with in situ metal immobilization by gravel sludge and red mud

    International Nuclear Information System (INIS)

    Iqbal, Muhammad; Puschenreiter, Markus; Oburger, Eva; Santner, Jakob; Wenzel, Walter W.

    2012-01-01

    As phytoextraction implementation may be limited by metal toxicity and leaching, we investigated the idea of in situ metal immobilization in bulk soil, while increasing metal bioavailability in the rhizosphere. Salix smithiana was grown in a pot experiment on two Cd/Zn polluted soils. Treatments with or without willows included: no additives; gravel sludge + red mud kg −1 ; acidification with S to pH 3.5; and metal immobilization combined with soil acidification. Salix smithiana removed up to 0.78 ± 0.06% total Cd and 0.34% (±0.02) total Zn from the non-treated soils. The phytoextraction efficiency in the S treatments was enhanced by up to ∼50% in response to metal solubility that was magnified by reductive co-dissolution from Mn (IV) and Fe(III) (oxy)hydroxides during microbial S oxidation in the willow rhizosphere. The proposed technique proved to enhance phytoextraction efficiency while controlling the risk of metal leaching from the root zone and phytotoxicity. - Highlights: ► Phytoextraction of Cd and Zn using willow (Salix smithiana) was enhanced by elemental sulfur. ► Reductive metals dissolution by microbial S oxidation was magnified in the willow rhizosphere. ► Thus metal bioavailability was high at the location of uptake in the phytoextraction crop. ► Concurrent immobilization by soil amendments can reduce metal leaching from the bulk soil. - Magnified Cd and Zn availability in S-amended rhizosphere of Salix smithiana enhances shoot Cd/Zn removal while metal leaching from the root zone can be reduced by immobilizing soil amendments.

  6. Uptake of cadmium from hydroponic solutions by willows ( Salix spp ...

    African Journals Online (AJOL)

    Salix integra 'Weishanhu') and Yizhibi (S. integra 'Yizhibi') were chosen as model plants to evaluate their potential for uptake of cadmium from hydroponic culture and relative uptake mechanism. Cadmium uptake showed a linear increase in the ...

  7. Significance of stigma receptivity in intergeneric cross-pollination of Salix × Populus

    Directory of Open Access Journals (Sweden)

    Elżbieta Zenkteler

    2016-09-01

    Full Text Available The pollen–stigma interaction plays an important role in reproductive process and has been continuously studied in many interspecific and intergeneric crossing experiments. The aim of this study was to investigate stigma receptivity (SR of willow in order to determine the most suitable period for its pollination with poplar pollen and improve the effectiveness of Salix × Populus crosses. Tissue samples were examined histologically using light, epifluorescent, scanning, and transmission electron microscopy. Willow SR was determined by stigma morphological traits, test of pollen germination rate, Peroxtesmo test of peroxidase and esterase activity on stigma surface as well as papilla ultrastructure at anthesis. We have ascertained that the SR duration in willow is short, lasting from 1 to 2 DA. The poplar pollen germination rate on willow stigmas on 1 DA ranged from 26.3 to 11.2%.

  8. Evaluation of the phytoremediation potential of the Salix caprea in tailing ponds

    Directory of Open Access Journals (Sweden)

    Anca MIHALY-COZMUTA

    2009-05-01

    Full Text Available The goat willow (Salix caprea belongs to the typical pioneer vegetation at former ore mining sites in East and Middle Europe as a result of its high metal-tolerance. Ectomycorrhizal (EM fungi are known to contribute essentially to the tolerance of plants against abiotic stress. We have determined the absorption/ accretion potential of the goat willow related to heavy metals, as well as the growth rhythm of this species in conditions offered by polluted sites, correlated to the presence/ absence of the microbiota in the soil attached to the root. The research unravels how the goat willow (Salix caprea, as a native species can be used in remediation actions of polluted sites based on its high adaptive potential, as well as on its capacity to absorb and accumulate heavy metals. In terms of survival strategy, mycorhrised fungi attached to the polluted soil play a significant part, since they induce plants a certain resistance by diminishing the absorption of heavy metals by the host plant. We suppose that the majority of EM associations of S. caprea, in combination with high portions of metal-chelating organic acids containing fungal partners, contributes essentially to the high tolerance of this species against high metal concentrations in the soil.

  9. Evaluation of salix farmers experiences and attitudes to growing; Utvaerdering av salixodlares erfarenheter och attityder till odling

    Energy Technology Data Exchange (ETDEWEB)

    Paulrud, Susanne; Roennbaeck, Marie (Swedish National Testing and Research Inst., Boraas (Sweden)); Gunnarsson, Carina; Olsson, Johanna (Swedish Inst. of Agricultural and Environmental Engineering, Uppsala (Sweden))

    2010-11-15

    Approximately 16 000 hectares of salix (willow) were planted in Sweden in the early 1990s, driven by subsidies and optimistic market expectations. Today the salix plantations have decreased to approximately 12000 hectares. In this study 29 salix farmers and four entrepreneurs that work with salix harvest were interviewed to find out more about their attitudes to, and experiences of growing salix. Most of the farmers interviewed were not satisfied with their salix plantation. The reasons given were the low profitability and problems with the harvest systems. Key reasons for the low profitability are cited as: old plantations that are planted on poor soil; lack of fertilization; an ineffective organization to taking care of the harvest and to sell the salix chips; and a low price for the salix chips. In addition the system for harvesting needs to be more flexible and there is a need for machine systems adapted for harvest of smaller fields

  10. Pathogenic and Ice-Nucleation Active (INA) Bacteria causing Dieback of Willows in Short Rotation Forestry

    Energy Technology Data Exchange (ETDEWEB)

    Nejad, Pajand

    2005-03-01

    To find out whether bacteria isolated from diseased plant parts can be the main causal agent for the dieback appearing in Salix energy forestry plantations in Sweden during the last few years, and if the joint effects of bacteria and frost injury are synergistic, extensive sampling of shoots from diseased Salix plants was performed. We performed several laboratory and greenhouse investigations and used evaluation techniques on the functions of the Ice-Nucleation Active (INA) bacteria. We carried out a comparison between spring and autumn bacterial communities isolated from within (endophytically) and surface (epiphytically) plant tissues of Salix viminalis. Seasonal variation of bacteria in willow clones with different levels of frost sensitivity and symptoms of bacterial damage was also investigated. We further focussed on possible effect of fertilisation and nutrient availability on the bacterial community in relation to plant dieback in Estonian willow plantations. The identification and detection of INA bacteria which cause damage in combination with frost to willow (Salix spp) plants in late fall, winter and spring was performed using BIOLOG MicroPlate, biochemical tests, selective INA primers and 16S rDNA analysis. To distinguish the character for differentiation between these bacteria morphologically and with respect to growing ability different culture media were used. We studied the temperature, at which ice nucleation occurred for individual bacteria, estimated the population of INA bacteria, effect of growth limiting factors, and evaluated the effect of chemical and physical agents for disruption and possible inhibition of INA among individual bacterial strains. The concentration of carbon, nitrogen and phosphorus on INA is discussed. We demonstrate that among the bacterial isolates recovered from the willow plantations, there were many that were capable of ice nucleation at temperatures between -2 and -10 deg C, many that were capable of inducing a

  11. Nutrient Concentrations of Bush Bean (Phaseolus vulgaris L. and Potato (Solanum tuberosum L. Cultivated in Subarctic Soils Managed with Intercropping and Willow (Salix spp. Agroforestry

    Directory of Open Access Journals (Sweden)

    Meaghan J. Wilton

    2017-12-01

    Full Text Available To ease food insecurities in northern Canada, some remote communities started gardening initiatives to gain more access to locally grown foods. Bush beans (Phaseolus vulgaris L. and potatoes (Solanum tuberosum L. were assessed for N, P, K, Mg, and Ca concentrations of foliage as indicators of plant nutrition in a calcareous silty loam soil of northern Ontario James Bay lowlands. Crops were grown in sole cropping and intercropping configurations, with comparisons made between an open field and an agroforestry site enclosed with willow (Salix spp. trees. Foliage chemical analysis of the sites revealed an abundance of Ca, adequacies for Mg and N, and deficiencies in P and K. Intercropping bean and potato did not show significant crop–crop facilitation for nutrients. The agroforestry site showed to be a superior management practice for the James Bay lowland region, specifically for P. The agroforestry site had significantly greater P for bean plant (p = 0.024 and potato foliage (p = 0.002 compared to the open site. It is suspected that the presence of willows improve plant available P to bean and potatoes by tree root—crop root interactions and microclimate enhancements.

  12. Evaluating growth effects from an imidacloprid treatment in black willow and eastern cottonwood cuttings

    Science.gov (United States)

    Luciano de Sene Fernandes; Ray A. Souter; Theodor D. Leininger

    2015-01-01

    Black willow (Salix nigra Marsh.) and eastern cottonwood (Populus deltoides Bartram ex Marsh.), two species native in the Lower Mississippi Alluvial Valley, have importance in short rotation woody crop (SRWC) systems for biomass production (Ruark 2006).

  13. Feeding preferences of the willow sawfly Nematus oligospilus (Hymenoptera: Tenthredinidae for commercial Salix clones

    Directory of Open Access Journals (Sweden)

    Verónica LOETTI

    2012-01-01

    Full Text Available Nematus oligospilus Förster (Hymenoptera: Tenthredinidae, o la avispa sierra de los sauces, es nativa del Hemisferio Norte y se ha convertido en un serio defoliador en plantaciones de sauces (Salix spp. del Hemisferio Sur, después de su introducción a principios de 1980. Los estudios sobre las preferencias de hospedador aportan información útil para el desarrollo de estrategias, donde la avispa sierra puede producir daño a los árboles y pérdidas económicas. Se evaluó la preferencia alimentaria de las larvas de N. oligospilus, mediante ensayos de laboratorio; se ofrecieron en forma simultánea hojas de cuatro clones de sauce, usados comúnmente en plantaciones comerciales en Argentina (Salix babylonica var sacramenta Hortus, Salix nigra Marsch., S. babylonica L. x Salix alba L. 131-27 and Salix matsudana Koidz. x S. alba L. 13-44. Las larvas de N. oligospilus se alimentaron de las hojas de los cuatro clones. Sin embargo, consumieron una proporción significativamente mayor de las hojas de S. babylonica var sacramenta. Estos resultados indican que todos los clones utilizados en los ensayos fueron palatables para el insecto y que S. babylonica var sacramenta es el hospedador preferido para la herbivoría de las larvas.

  14. Bottom-up factors influencing riparian willow recovery in Yellowstone National Park

    Science.gov (United States)

    Tercek, M.T.; Stottlemyer, R.; Renkin, R.

    2010-01-01

    After the elimination of wolves (Canis lupis L.) in the 1920s, woody riparian plant communities on the northern range of Yellowstone National Park (YNP) declined an estimated 50%. After the reintroduction of wolves in 19951996, riparian willows (Salix spp.) on YNP's northern range showed significant growth for the first time since the 1920s. However, the pace of willow recovery has not been uniform. Some communities have exceeded 400 cm, while others are still at pre-1995 levels of 250 cm max. height) willow sites where willows had escaped elk (Cervus elaphus L.) browsing with "short" willow sites that could still be browsed. Unlike studies that manipulated willow height with fences and artificial dams, we examined sites that had natural growth differences in height since the reintroduction of wolves. Tall willow sites had greater water availability, more-rapid net soil nitrogen mineralization, greater snow depth, lower soil respiration rates, and cooler summer soil temperatures than nearby short willow sites. Most of these differences were measured both in herbaceous areas adjacent to the willow patches and in the willow patches themselves, suggesting that they were not effects of varying willow height recovery but were instead preexisting site differences that may have contributed to increased plant productivity. Our results agree with earlier studies in experimental plots which suggest that the varying pace of willow recovery has been influenced by abiotic limiting factors that interact with top-down reductions in willow browsing by elk. ?? 2010 Western North American Naturalist.

  15. Clonal variation in survival and growth of hybrid poplar and willow in an in situ trial on soils heavily contaminated with petroleum hydrocarbons

    Science.gov (United States)

    Ronald S., Jr. Zalesny; Edmund O. Bauer; Richard B. Hall; Jill A. Zalesny; Joshua Kunzman; Chris J. Rog; Don E. Riemenschneider

    2005-01-01

    Species and hybrids between species belonging to the genera Populus (poplar) and Salix (willow) have been used successfully for phytoremediation of contaminated soils. Our objectives were to: 1) evaluate the potential for establishing genotypes of poplar and willow on soils heavily contaminated with petroleum hydrocarbons and 2)...

  16. Phytotoxicity of Sodium Fluoride and Uptake of Fluoride in Willow Trees

    DEFF Research Database (Denmark)

    Clausen, Lauge Peter Westergaard; Gosewinkel Karlson, Ulrich; Trapp, Stefan

    2015-01-01

    The willow tree (Salix viminalis) toxicity test and a cress seed germination test (Lepidium sativum) were used to determine uptake and phytotoxicity of NaF. Concentrations in hydroponic solutions were 0-1000 mg F/L and 0-400 mg F/L in the preliminary and definitive test. A third test was done...

  17. Sampling procedure in a willow plantation for chemical elements important for biomass combustion quality

    DEFF Research Database (Denmark)

    Liu, Na; Nielsen, Henrik Kofoed; Jørgensen, Uffe

    2015-01-01

    clone ‘Tordis’, and to reveal the relationship between sampling position, shoot diameters, and distribution of elements. Five Tordis willow shoots were cut into 10–50 cm sections from base to top. The ash content and concentration of twelve elements (Al, Ca, Cd, Cu, Fe, K, Mg, Mn, Na, P, Si, and Zn......Willow (Salix spp.) is expected to contribute significantly to the woody bioenergy system in the future, so more information on how to sample the quality of the willow biomass is needed. The objectives of this study were to investigate the spatial variation of elements within shoots of a willow......) in each section were determined. The results showed large spatial variation in the distribution of most elements along the length of the willow shoots. Concentrations of elements in 2-year old shoots of the willow clone Tordis were fairly stable within the range of 100–285 cm above ground and resembled...

  18. Community outreach and education: key components of the Salix consortium's willow biomass project

    International Nuclear Information System (INIS)

    Volk, T.A.; Edick, S.; Brown, S.; Downing, M.

    1999-01-01

    This project facilitates the commercialization of willow biomass crops as a locally grown source of renewable energy. The challenge is to simultaneously optimize production and utilization technology, develop farmer interest and crop acreage, and establish stable and reliable markets. The participation of farmers and landowners, businesses, and local and regional governments in the process is essential for success. A three-phased approach elicits this participation: focused outreach and education, active involvement of potential producers of willow biomass crops, and the development of a user-friendly economic and business model that can be used by a variety of stakeholders. Barriers to commercialization have been identified, such as misconceptions about the production system and crop, assurances of a stable and reliable market for the material, and indications that the equipment and infrastructure to grow and process willow biomass crops are in place. Outreach efforts have specifically addressed these issues. As a result target audiences' responses have changed from passive observation to inquiries and suggestions for active participation. This shift represents a significant step towards the goal of making willow biomass crops a viable source of locally produced fuel. (author)

  19. Test of aerobic TCE degradation by willows (Salix viminalis) and willows inoculated with TCE-cometabolizing strains of Burkholderia cepacia.

    Science.gov (United States)

    Clausen, Lauge Peter Westergaard; Broholm, Mette Martina; Gosewinkel, Ulrich; Trapp, Stefan

    2017-08-01

    Trichloroethylene (TCE) is a widespread soil and groundwater pollutant and clean-up is often problematic and expensive. Phytoremediation may be a cost-effective solution at some sites. This study investigates TCE degradation by willows (S. viminalis) and willows inoculated with three strains of B. cepacia (301C, PR1-31 and VM1330-pTOM), using chloride formation as an indicator of dehalogenation. Willows were grown in non-sterile, hydroponic conditions for 3 weeks in chloride-free nutrient solution spiked with TCE. TCE was added weekly due to rapid loss by volatilization. Chloride and TCE in solution were measured every 2-3 days and chloride and metabolite concentrations in plants were measured at test termination. Based on transpiration, no tree toxicity of TCE exposure was observed. However, trees grown in chloride-free solution showed severely inhibited transpiration. No or very little chloride was formed during the test, and levels of chloride in TCE-exposed trees were not elevated. Chloride concentrations in chloride containing TCE-free nutrient solution doubled within 23 days, indicating active exclusion of chloride by root cell membranes. Only traces of TCE-metabolites were detected in plant tissue. We conclude that TCE is not, or to a limited extent (less than 3%), aerobically degraded by the willow trees. The three strains of B. cepacia did not enhance TCE mineralization. Future successful application of rhizo- and phytodegradation of TCE requires measures to be taken to improve the degradation rates.

  20. The potential of willow for remediation of heavy metal polluted calcareous urban soils

    International Nuclear Information System (INIS)

    Jensen, Julie K.; Holm, Peter E.; Nejrup, Jens; Larsen, Morten B.; Borggaard, Ole K.

    2009-01-01

    Growth performance and heavy metal uptake by willow (Salix viminalis) from strongly and moderately polluted calcareous soils were investigated in field and growth chamber trials to assess the suitability of willow for phytoremediation. Field uptakes were 2-10 times higher than growth chamber uptakes. Despite high concentrations of cadmium (≥80 mg/kg) and zinc (≥3000 mg/kg) in leaves of willow grown on strongly polluted soil with up to 18 mg Cd/kg, 1400 mg Cu/kg, 500 mg Pb/kg and 3300 mg Zn/kg, it is unsuited on strongly polluted soils because of poor growth. However, willow proved promising on moderately polluted soils (2.5 mg Cd/kg and 400 mg Zn/kg), where it extracted 0.13% of total Cd and 0.29% of the total Zn per year probably representing the most mobile fraction. Cu and Pb are strongly fixed in calcareous soils. - Willow is suited for remediation of moderately heavy metal polluted calcareous soils

  1. Ethyl lactate-EDTA composite system enhances the remediation of the cadmium-contaminated soil by Autochthonous Willow (Salix x aureo-pendula CL 'J1011') in the lower reaches of the Yangtze River

    International Nuclear Information System (INIS)

    Li Jiahua; Sun Yuanyuan; Yin Ying; Ji Rong; Wu Jichun; Wang Xiaorong; Guo Hongyan

    2010-01-01

    In order to explore a practical approach to the remediation of the cadmium (Cd)-contaminated soil in the lower reaches of the Yangtze River, we evaluated the effects of a local willow (Salix x aureo-pendula CL 'J1011') of absorbing, accumulating, and translocating Cd; and assessed the potential of chelator ethylenediaminetetraacetic acid (EDTA) in combination with ethyl lactate for enhancing the efficiency of the willow in removing Cd in two water-culture growth chamber trials and a field one. The willow showed a high tolerance to Cd in growth chamber trial 1 where the Cd concentration in the medium reached up to 25 mg L -1 medium, and the bioaccumulation factors (BAFs) of the shoots for Cd rose from 3.8 to 7.4 as the Cd concentration in the medium was elevated from 5 to 25 mg L -1 medium. In growth chamber trial 2, the average Cd removal rates in two treatments with EDTA and ethyl lactate (molar ratios of EDTA to ethyl lactate = 68/39 and 53.5/53.5, respectively) reached 0.71 mg d -1 pot -1 for the duration of Day 5-8 and 0.59 mg d -1 pot -1 for that of Day 8-11, which were 5- and 4-fold of their counterparts in the control, respectively. In the field trial, for the remediational duration of 45 days, three treatments-willow alone, willow combined with EDTA, and willow combined with EDTA and ethyl lactate-led to decreases in the Cd concentration in soil by 5%, 20%, and 29%, respectively; increases in that in the leaves by 14.6%, 56.7%, and 146.5%, respectively; and increases in that in the stems by 15.6%, 41.2%, and 87.4%, respectively, compared to their counterparts on Day 0. These results indicate that EDTA combined with ethyl lactate significantly enhanced the efficiency of willow in removing Cd from the soil. Therefore, a phytoextration system consisting of the autochthonous willow, EDTA, and ethyl lactate has high potential for the remediation of the Cd-polluted soil in the lower reaches of the Yangtze River.

  2. Ethyl lactate-EDTA composite system enhances the remediation of the cadmium-contaminated soil by autochthonous willow (Salix x aureo-pendula CL 'J1011') in the lower reaches of the Yangtze River.

    Science.gov (United States)

    Li, Jiahua; Sun, Yuanyuan; Yin, Ying; Ji, Rong; Wu, Jichun; Wang, Xiaorong; Guo, Hongyan

    2010-09-15

    In order to explore a practical approach to the remediation of the cadmium (Cd)-contaminated soil in the lower reaches of the Yangtze River, we evaluated the effects of a local willow (Salix x aureo-pendula CL 'J1011') of absorbing, accumulating, and translocating Cd; and assessed the potential of chelator ethylenediaminetetraacetic acid (EDTA) in combination with ethyl lactate for enhancing the efficiency of the willow in removing Cd in two water-culture growth chamber trials and a field one. The willow showed a high tolerance to Cd in growth chamber trial 1 where the Cd concentration in the medium reached up to 25 mg L(-1) medium, and the bioaccumulation factors (BAFs) of the shoots for Cd rose from 3.8 to 7.4 as the Cd concentration in the medium was elevated from 5 to 25 mg L(-1) medium. In growth chamber trial 2, the average Cd removal rates in two treatments with EDTA and ethyl lactate (molar ratios of EDTA to ethyl lactate=68/39 and 53.5/53.5, respectively) reached 0.71 mg d(-1) pot(-1) for the duration of Day 5-8 and 0.59 mg d(-1) pot(-1) for that of Day 8-11, which were 5- and 4-fold of their counterparts in the control, respectively. In the field trial, for the remediational duration of 45 days, three treatments-willow alone, willow combined with EDTA, and willow combined with EDTA and ethyl lactate-led to decreases in the Cd concentration in soil by 5%, 20%, and 29%, respectively; increases in that in the leaves by 14.6%, 56.7%, and 146.5%, respectively; and increases in that in the stems by 15.6%, 41.2%, and 87.4%, respectively, compared to their counterparts on Day 0. These results indicate that EDTA combined with ethyl lactate significantly enhanced the efficiency of willow in removing Cd from the soil. Therefore, a phytoextration system consisting of the autochthonous willow, EDTA, and ethyl lactate has high potential for the remediation of the Cd-polluted soil in the lower reaches of the Yangtze River. Copyright 2010 Elsevier B.V. All rights

  3. Removal of 4-chlorobenzoic acid from spiked hydroponic solution by willow trees (Salix viminalis)

    DEFF Research Database (Denmark)

    Deavers, K.; Macek, T.; Karlson, U.

    2010-01-01

    . Methods The removal of 4-CBA by willow trees was investigated with intact, septic willow trees growing in hydroponic solution and with sterile cell suspensions at concentrations of 5 mg/L and 50 mg/L 4-CBA. Nutrient solutions with different levels of ammonium and nitrate were prepared to achieve different...

  4. Competition favors elk over beaver in a riparian willow ecosystem

    Science.gov (United States)

    Baker, B.W.; Peinetti, H.R.; Coughenour, M.C.; Johnson, T.L.

    2012-01-01

    Beaver (Castor spp.) conservation requires an understanding of their complex interactions with competing herbivores. Simulation modeling offers a controlled environment to examine long-term dynamics in ecosystems driven by uncontrollable variables. We used a new version of the SAVANNA ecosystem model to investigate beaver (C. Canadensis) and elk (Cervus elapses) competition for willow (Salix spp.). We initialized the model with field data from Rocky Mountain National Park, Colorado, USA, to simulate a 4-ha riparian ecosystem containing beaver, elk, and willow. We found beaver persisted indefinitely when elk density was or = 30 elk km_2. The loss of tall willow preceded rapid beaver declines, thus willow condition may predict beaver population trajectory in natural environments. Beaver were able to persist with slightly higher elk densities if beaver alternated their use of foraging sites in a rest-rotation pattern rather than maintained continuous use. Thus, we found asymmetrical competition for willow strongly favored elk over beaver in a simulated montane ecosystem. Finally, we discuss application of the SAVANNA model and mechanisms of competition relative to beaver persistence as metapopulations, ecological resistance and alternative state models, and ecosystem regulation.

  5. The role and significance of Salix plantations for energy in Swedish agriculture

    Energy Technology Data Exchange (ETDEWEB)

    Forsse, L.S.; Ledin, S.; Johansson, H. [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden)

    1993-12-31

    Fifteen years of research and development of energy forestry with Salix species has lead to a firm basis of knowledge concerning the basic biology, stand ecology and production systems of fast growing willows in Sweden. The biology research program continues to emphasize studies of plant biology and diseases as well as areas such as clone/site interactions, mixed clonal plantations and breeding. The technological research and development concentrates on functional and effective machinery for planting, harvesting, etc. Recently Salix plantations for energy production in Sweden have been commercialized. Plantations start with 18,000 cuttings of willow clones per hectare. During the first summer weed control is the most important treatment. Fertilizers are applied to keep a high production level. Crops are harvested during winter at 3--5 year intervals. The average annual production is about 10--12 tonnes DM per hectare. The life of Salix plantations is estimated as 25--30 years. An estimated potential of 300,000 hectares of Salix plantations would result 5% of the energy needs in Sweden. Wood fuel from the conventional forest equals 60 TWh today, with a potential of being doubled within 10--20 years. The economic outcome for the farmer of growing Salix mainly depends on the price of chips and the level of production. A fundamental requirement for establishing plantations is that there is a wood fuel market within a reasonable distance (about 50 km). In a calculation stretching over a period of 24 years with a production level of 12 tonnes DM per hectare and year, and at an interest rate of 6%, the net return is about 1,000--1,500 SEK/ha/yr (about 7 SEK/US$) if simultaneous chipping is used. With separate harvest and chipping and enterprise in this calculation breaks even. Interest in the utilization of sludge, ash, waste water and leakage water as nutrients for energy forests is increasing from local and regional authorities.

  6. Effect of temperature on the uptake and metabolism of cyanide by weeping willows

    DEFF Research Database (Denmark)

    Yu, X.-Z.; Trapp, Stefan; Zhou, P.-H.

    2007-01-01

    Plants’ uptake and metabolism of cyanide in response to changes in temperature was investigated. Pre-rooted weeping willows (Salix babylonica L.) were exposed to hydroponic solution spiked with potassium cyanide for 2–3 d. Ten different temperatures were used, ranging from 11◦C to 32◦C. Cyanide...

  7. Phytotoxicity of polycyclic aromatic hydrocarbons to willow trees

    Energy Technology Data Exchange (ETDEWEB)

    Thygesen, R.S.; Trapp, S. [Environment and Resources DTU, Technical Univ. of Denmark, Lyngby (Denmark)

    2002-07-01

    The toxicity of PAH to willow trees (Salix alba, S. viminalis, S. viminalis x schwerinii) was investigated. Willow cuttings were grown in PAH-saturated hydroponic solution (naphthalene NAP, phenanthrene PHEN and benzo(a)pyrene BaP). Toxicity was related to aqueous solubility and was highest for NAP. PHEN did not show significant effects, except in one case. Exposure of trees to BaP showed no effect in two cases, but increased transpiration and growth in two others. High dosages of NAP were fatal for the trees, the lowest dosage significantly stimulated growth. Soil samples were taken from several PAH contaminated sites, among them gas works sites and a former sludge basin. The PAH contents ranged from 1.76 mg/kg to 1451 mg/kg. None of the soils was lethally toxic to the trees, and difference between growth in control soils and growth in PAH contaminated soils was not apparent. Growth and water use efficiency were positively, but not significantly correlated to the PAH content of the soils. Outdoor growth of willows and poplars on the former sludge basin in Valby was monitored, with willows growing faster than poplars (Populus trichocarpa). Phytotoxic effects could be observed at some willows at the Valby sludge basin, but it is not sure whether these effects can be contributed to PAH. (orig.)

  8. Treatment of log yard run-off by irrigation of grass and willows

    International Nuclear Information System (INIS)

    Jonsson, Maria; Dimitriou, Ioannis; Aronsson, Paer; Elowson, Torbjoern

    2006-01-01

    Log yard run-off is a potential environmental risk, among other things because it creates an oxygen deficiency in receiving watercourses. This study was conducted to investigate the purification efficiency of soil-plant systems with couchgrass (Elymus repens) and willows (Salix sp.) when intensively irrigated with run-off from an open sprinkling system at a Norway spruce (Picea abies) log yard. The purification efficiency was determined both at the field scale (couchgrass) and in 68-L lysimeters (couchgrass and willows). Groundwater in the field and drainage water from the lysimeters were analysed for Total Organic Carbon (TOC), distillable phenols, total P, and total N. Retention of TOC, phenols and P occurred but no difference between couchgrass and willows was observed. The system had better purification capacity at the field scale than in the lysimeters. -- By irrigating willow and couchgrass soil-plant systems with log yard run-off water, TOC, phenols, and phosphorus were reduced with 35% to 96% in the water

  9. Phytoremediation prospects of willow stands on contaminated sediment: a field trial

    International Nuclear Information System (INIS)

    Vervaeke, P.; Luyssaert, S.; Mertens, J.; Meers, E.; Tack, F.M.G.; Lust, N.

    2003-01-01

    A field trial indicated increased degradation of mineral oil in sediments planted with willow. - Establishing fast growing willow stands on land disposed contaminated dredged sediment can result in the revaluation of this material and opens possibilities for phytoremediation. A field trial was designed to assess the impact of planting a willow stand (Salix viminalis L. 'Orm') on the dissipation of organic contaminants (mineral oil and PAHs) in dredged sediment. In addition, the accumulation of heavy metals (Cd, Cu, Pb and Zn) in the biomass was determined. After 1.5 years, a significant decrease of 57% in the mineral oil concentration in the sediment planted with willow was observed. Degradation of mineral oil in sediment which was left fallow, was only 15%. The mineral oil degradation under willow was most pronounced (79%) in the root zone of the stand. In the sediment which was left fallow there was a significant reduction of the total PAH content by 32% compared with a 23% reduction in the planted sediment. The moderate and selective metal uptake, measured in this study, limits the prospects for phytoextraction of metals from dredged sediment

  10. Phytoremediation prospects of willow stands on contaminated sediment: a field trial

    Energy Technology Data Exchange (ETDEWEB)

    Vervaeke, P.; Luyssaert, S.; Mertens, J.; Meers, E.; Tack, F.M.G.; Lust, N

    2003-11-01

    A field trial indicated increased degradation of mineral oil in sediments planted with willow. - Establishing fast growing willow stands on land disposed contaminated dredged sediment can result in the revaluation of this material and opens possibilities for phytoremediation. A field trial was designed to assess the impact of planting a willow stand (Salix viminalis L. 'Orm') on the dissipation of organic contaminants (mineral oil and PAHs) in dredged sediment. In addition, the accumulation of heavy metals (Cd, Cu, Pb and Zn) in the biomass was determined. After 1.5 years, a significant decrease of 57% in the mineral oil concentration in the sediment planted with willow was observed. Degradation of mineral oil in sediment which was left fallow, was only 15%. The mineral oil degradation under willow was most pronounced (79%) in the root zone of the stand. In the sediment which was left fallow there was a significant reduction of the total PAH content by 32% compared with a 23% reduction in the planted sediment. The moderate and selective metal uptake, measured in this study, limits the prospects for phytoextraction of metals from dredged sediment.

  11. The Willow Microbiome is Influenced by Soil Petroleum-Hydrocarbon Concentration with Plant Compartment-Specific Effects

    Directory of Open Access Journals (Sweden)

    Stacie Tardif

    2016-09-01

    Full Text Available The interaction between plants and microorganisms, which is the driving force behind the decontamination of petroleum hydrocarbon (PHC contamination in phytoremediation technology, is poorly understood. Here, we aimed at characterizing the variations between plant compartments in the microbiome of two willow cultivars growing in contaminated soils. A field experiment was set-up at a former petrochemical plant in Canada and, after two growing seasons, bulk soil, rhizosphere soil, roots and stems samples of two willow cultivars (Salix purpurea cv. FishCreek and Salix miyabeana cv. SX67 growing at three PHC contamination concentrations were taken. DNA was extracted and bacterial 16S rRNA gene and fungal internal transcribed spacer (ITS regions were amplified and sequenced using an Ion Torrent Personal Genome Machine. Following multivariate statistical analyses, the level of PHC-contamination appeared as the primary factor influencing the willow microbiome with compartment-specific effects, with significant differences between the responses of bacterial and fungal communities. Increasing PHC contamination levels resulted in shifts in the microbiome composition, favoring putative hydrocarbon degraders and microorganisms previously reported as associated with plant health. These shifts were less drastic in the rhizosphere, root and stem tissues as compared to bulk soil, probably because the willows provided a more controlled environment and thus protected microbial communities against increasing contamination levels. Insights from this study will help to devise optimal plant microbiomes for increasing the efficiency of phytoremediation technology.

  12. Concentrations of Chemical Elements in Willow Biomass Depend on Clone, Site and Management in the Field

    DEFF Research Database (Denmark)

    Liu, Na; Jørgensen, Uffe; Lærke, Poul Erik

    2016-01-01

    Eight willow (Salix) clones (Inger, Klara, Linnea, Resolution, Stina, Terra Nova, Tora, Tordis) were planted on two soil types in Denmark. The biomass quality was evaluated after 3 years of growth by measuring differences in concentrations of 14 elements associated with ash behavior during combus...

  13. Ethyl lactate-EDTA composite system enhances the remediation of the cadmium-contaminated soil by Autochthonous Willow (Salix x aureo-pendula CL 'J1011') in the lower reaches of the Yangtze River

    Energy Technology Data Exchange (ETDEWEB)

    Li Jiahua [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Sun Yuanyuan [State Key Laboratory of Pollution Control and Resource Reuse, Department of Hydrosciences, Nanjing University, Nanjing 210093 (China); Yin Ying; Ji Rong [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Wu Jichun [State Key Laboratory of Pollution Control and Resource Reuse, Department of Hydrosciences, Nanjing University, Nanjing 210093 (China); Wang Xiaorong [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Guo Hongyan, E-mail: hyguo@nju.edu.cn [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China)

    2010-09-15

    In order to explore a practical approach to the remediation of the cadmium (Cd)-contaminated soil in the lower reaches of the Yangtze River, we evaluated the effects of a local willow (Salix x aureo-pendula CL 'J1011') of absorbing, accumulating, and translocating Cd; and assessed the potential of chelator ethylenediaminetetraacetic acid (EDTA) in combination with ethyl lactate for enhancing the efficiency of the willow in removing Cd in two water-culture growth chamber trials and a field one. The willow showed a high tolerance to Cd in growth chamber trial 1 where the Cd concentration in the medium reached up to 25 mg L{sup -1} medium, and the bioaccumulation factors (BAFs) of the shoots for Cd rose from 3.8 to 7.4 as the Cd concentration in the medium was elevated from 5 to 25 mg L{sup -1} medium. In growth chamber trial 2, the average Cd removal rates in two treatments with EDTA and ethyl lactate (molar ratios of EDTA to ethyl lactate = 68/39 and 53.5/53.5, respectively) reached 0.71 mg d{sup -1}pot{sup -1} for the duration of Day 5-8 and 0.59 mg d{sup -1}pot{sup -1} for that of Day 8-11, which were 5- and 4-fold of their counterparts in the control, respectively. In the field trial, for the remediational duration of 45 days, three treatments-willow alone, willow combined with EDTA, and willow combined with EDTA and ethyl lactate-led to decreases in the Cd concentration in soil by 5%, 20%, and 29%, respectively; increases in that in the leaves by 14.6%, 56.7%, and 146.5%, respectively; and increases in that in the stems by 15.6%, 41.2%, and 87.4%, respectively, compared to their counterparts on Day 0. These results indicate that EDTA combined with ethyl lactate significantly enhanced the efficiency of willow in removing Cd from the soil. Therefore, a phytoextration system consisting of the autochthonous willow, EDTA, and ethyl lactate has high potential for the remediation of the Cd-polluted soil in the lower reaches of the Yangtze River.

  14. Can energy willow (Salix sp.) remediate cadmium- and nickel-contaminated fish farm sludge?

    DEFF Research Database (Denmark)

    Pedersen, Marianne Bruus

    it meets the criteria. Phytoremediation by willow may combine accumulation of cadmium and nickel from the sludge with the production of an energy crop. The ability of eight selected willow clones to take up and tolerate cadmium and nickel was studied in pots under outdoor conditions. Fish farm sludge...

  15. Willow vegetation filters: Principles, results and economy

    International Nuclear Information System (INIS)

    Perttu, K.

    1996-01-01

    During recent years, it has become obvious that it is both environmentally and economically appropriate to use vegetation filters of different short rotation willows (Salix spp.) to purify water and soil. Swedish experiences of vegetation filter efficiencies have been demonstrated in several laboratory, field lysimeter, and full-scale experiments. However, there are still many questions to be answered, for example, how the uptake and allocation mechanisms of heavy metals and recalcitrant organic constituents function, or which maximum doses are possible in a particular situation without any risk of leachate losses. As far as plant nutrition is concerned, the past two decades of integrated research in Sweden have demonstrated that the willows have capacity for efficient uptake both of macro and micro nutrients, which is reflected in their high productivity. The purpose of this paper is to present some results on how vegetation filter stands of willow, irrigated with municipal wastewater, can function as purification plants, while at the same time producing fuelwood. This twofold utilization benefits both the environment and the economy. Treatment plants for wastewater purification using various types of vegetation filters have been tried in Sweden. The experiences consider both the nutrient and heavy metal uptake, and the whole process chain from establishment, cultivation and harvesting of the wastewater irrigated willow stands, to the utilization of the wood in heating plants. 33 refs, 5 tabs

  16. Assessment of Salix spp. pollen availability to insects based on aerobiological investigations

    Directory of Open Access Journals (Sweden)

    Elżbieta Weryszko-Chmielewska

    2017-06-01

    Full Text Available Pollen and nectar produced by flowers of species from the genus Salix are an important source of food for various insect groups in early spring. Most willows are entomophilous species; however, substantial amounts of airborne Salix pollen can be noted. The aim of the study was to evaluate the content of pollen of this taxon in the air of Lublin (central-east Poland in 2001–2016 and to identify the period of its greatest availability to insects. In 2015, we compared the course of the Salix pollen season in Lublin (51°14'37" N; 22°32'25" E and in the Roztoczański National Park (50°34'57" N; 23°04'24" E, Poland. We found that the date of the pollen season onset fluctuated greatly between March 16 and April 17. The greatest availability of Salix pollen to insects was noted from the end of the first 10-day-period of April to the first 10-day-period of May. The mean annual sum of airborne Salix pollen grains was 833. In Lublin, Salix pollen accounted for ca. 1.25% of the total airborne pollen content of different plant taxa. The investigations have demonstrated a 2-year cycle of Salix pollen abundance. The comparison of the pollen seasons in Lublin and in the Roztoczański National Park indicates that considerably greater amounts of pollen occur in the urban area than in the air of the Roztoczański National Park.

  17. Growth of Populus and Salix Species under Compost Leachate Irrigation

    Directory of Open Access Journals (Sweden)

    Tooba Abedi

    2014-12-01

    Full Text Available According to the known broad variation in remediation capacity, three plant species were used in the experiment: two fast growing poplar’s clones - Populus deltoides, Populus euramericana, and willows Salix alba. Populus and Salix cuttings were collected from the nursery of the Populus Research Center of Safrabasteh in the eastern part of Guilan province at north of Iran. The Populus clones were chosen because of their high biomass production capacity and willow- because it is native in Iran. The highest diameter growth rate was exhibited for all three plant species by the 1:1 treatment with an average of 0.26, 0.22 and 0.16 cm in eight months period for P. euroamericana, P. deltoides and S. alba, respectively. Over a period of eight months a higher growth rate of height was observed in (P and (1:1 treatment for S. alba (33.70 and 15.77 cm, respectively and in (C treatment for P. deltoides (16.51 cm. P. deltoides and S. alba produced significantly (p<0.05 smaller aboveground biomass in (P treatment compared to all species. P. deltoides exhibited greater mean aboveground biomass in the (1:1 treatment compared to other species. There were significant differences (p<0.05 in the growth of roots between P. deltoides, P. euramericana and S. alba in all of the treatments.

  18. Characterising willows for biomass and phytoremediation: growth, nitrogen and water use of 14 willow clones under different irrigation and fertilisation regimes

    International Nuclear Information System (INIS)

    Weih, Martin; Nordh, N.-E.

    2002-01-01

    Fourteen clones of willow (Salix spp.) were characterised in terms of growth, nitrogen and water-use efficiency under different irrigation and fertilisation treatments. Cuttings of willow clones, some commercially introduced and others new material, were pot-grown outdoors in Central Sweden under four experimental treatments in a full-factorial design. The experiment covered the period from bud-break until leaf abscission and the experimental conditions included two irrigation and two fertilisation regimes. The growth of the clones was evaluated in terms of relative growth rate and total biomass production of whole plants and shoots. Nitrogen (N) economy was studied by means of N productivity, N accumulation and N losses by leaf abscission. Water economy was analysed with respect to intrinsic water-use efficiency (foliar carbon isotope ratio; δ 13 C) and the capacity of leaves to retain water (relative water content). Significant differences between clones were found in nearly all parameters measured and the clones varied in the responses to the experimental treatments (clone x factor interaction effects). Thus, clone ranking often changed depending on the experimental treatment. The results are discussed with respect to clone selection for different willow applications such as biomass production and phytoremediation, and willow growth performance under different water and nutrient availabilities. The growth-physiological characterisation of young willows in the short term (several months) is regarded as a suitable approach for pre-selection of promising clones prior to extensive field evaluation

  19. Treatment of landfill leachate by irrigation of willow coppice - Plant response and treatment efficiency

    International Nuclear Information System (INIS)

    Aronsson, Paer; Dahlin, Torleif; Dimitriou, Ioannis

    2010-01-01

    Landfill leachates usually need to be treated before discharged, and using soil-plant systems for this has gained substantial interest in Sweden and in the UK. A three-year field study was conducted in central Sweden to quantify plant response, treatment efficiency and impact on groundwater quality of landfill leachate irrigation of short-rotation willow coppice (Salix). Two willow varieties were tested and four irrigation regimes in sixteen 400-m 2 plots. The willow plants did not react negatively, despite very high annual loads of nitrogen (≤2160 kg N/ha), chloride (≤8600 kg Cl/ha) and other elements. Mean annual growth was 1.5, 9.8 and 12.6 tonnes DM/ha during years 1-3. For one of two willow varieties tested, relative leaf length accurately predicted growth rate. Irrigation resulted in elevated groundwater concentrations of all elements applied. Treatment efficiency varied considerably for different elements, but was adequate when moderate loads were applied. - Short-rotation willow coppice was successfully used for treating a strong landfill leachate in central Sweden over three years.

  20. Comparison of willow and sunflower for uranium phytoextraction induced by citric acid

    International Nuclear Information System (INIS)

    Jan Mihalik; National Radiation Protection Institute, Prague; CZU, FAPPZ, Prague; Pavel Tlustos; Jirina Szakova

    2010-01-01

    This study deals with an efficiency of a low dose of citric acid soil application on phytoextraction of uranium. Willow (Salix spp.) and sunflower (Helianthus annus L.) were tested in this experiment with contaminated soil. The enhancing of uranium bioaccumulation was confirmed, but in contrast to previous studies, the highest quantity of uranium was accumulated in leaves. After 5 weeks of citric acid treatment, willow was more efficient in the uptake and translocation of uranium than sunflower. The transfer coefficient calculated for leaves increased from 0.033 (control) to 0.74, or 0.56 after five doses of 5 mmol of citric acid per 1 kg of soil for willow or sunflower, respectively. The uptake characterized by the total U content achieved 88 and 108 mg kg -1 in relation to the above ground parts of sunflower and willow, respectively. Even though both plants accumulated U in their above ground parts in significant rate, they employed diverse ways to achieve it. At the end of the treatment, the physiological condition of the plants enabled us to continue this method. (author)

  1. Water tables constrain height recovery of willow on Yellowstone's northern range.

    Science.gov (United States)

    Bilyeu, Danielle M; Cooper, David J; Hobbs, N Thompson

    2008-01-01

    Excessive levels of herbivory may disturb ecosystems in ways that persist even when herbivory is moderated. These persistent changes may complicate efforts to restore ecosystems affected by herbivores. Willow (Salix spp.) communities within the northern range in Yellowstone National Park have been eliminated or degraded in many riparian areas by excessive elk (Cervus elaphus L.) browsing. Elk browsing of riparian willows appears to have diminished following the reintroduction of wolves (Canis lupis L.), but it remains uncertain whether reduced herbivory will restore willow communities. The direct effects of elk browsing on willows have been accompanied by indirect effects from the loss of beaver (Castor canadensis Kuhl) activity, including incision of stream channels, erosion of fine sediments, and lower water tables near streams historically dammed by beaver. In areas where these changes have occurred, lowered water tables may suppress willow height even in the absence of elk browsing. We conducted a factorial field experiment to understand willow responses to browsing and to height of water tables. After four years of protection from elk browsing, willows with ambient water tables averaged only 106 cm in height, with negligible height gain in two of three study species during the last year of the experiment. Willows that were protected from browsing and had artificially elevated water tables averaged 147 cm in height and gained 19 cm in the last year of the experiment. In browsed plots, elevated water tables doubled height gain during a period of slightly reduced browsing pressure. We conclude that water availability mediates the rate of willow height gain and may determine whether willows grow tall enough to escape the browse zone of elk and gain resistance to future elk browsing. Consequently, in areas where long-term beaver absence has resulted in incised stream channels and low water tables, a reduction in elk browsing alone may not be sufficient for recovery

  2. Development of an applied black willow tree improvement program for biomass production in the south

    Science.gov (United States)

    Randall J. Rousseau; Emile S. Gardiner; Theodor D. Leininger

    2012-01-01

    The development of rapidly growing biomass woody crops is imperative as the United States strives to meet renewable energy goals. The Department of Energy has indicated that biomass is a prime source for renewable energy for the southern United States. Black Willow (Salix nigra Marsh.) is a potential bioenergy/biofuels crop for dedicated short-...

  3. In vitro regeneration of Salix nigra from adventitious shoots.

    Science.gov (United States)

    Lyyra, Satu; Lima, Amparo; Merkle, Scott A

    2006-07-01

    Black willow (Salix nigra Marsh.) is the largest and only commercially important willow species in North America. It is a candidate for phytoremediation of polluted soils because it is fast-growing and thrives on floodplains throughout eastern USA. Our objective was to develop a protocol for the in vitro regeneration of black willow plants that could serve as target material for gene transformation. Unexpanded inflorescence explants were excised from dormant buds collected from three source trees and cultured on woody plant medium (WPM) supplemented with one of: (1) 0.1 mg l(-1) thidiazuron (TDZ); (2) 0.5 mg l(-1) 6-benzoaminopurine (BAP); or (3) 1 mg l(-1) BAP. All plant growth regulator (PGR) treatments induced direct adventitious bud formation from the genotypes. The percentage of explants producing buds ranged from 20 to 92%, depending on genotype and treatment. Although most of the TDZ-treated inflorescences produced buds, these buds failed to elongate into shoots. Buds on explants treated with BAP elongated into shoots that were easily rooted in vitro and further established in potting mix in high humidity. The PGR treatments significantly affected shoot regeneration frequency (P < 0.01). The highest shoot regeneration frequency (36%) was achieved with Genotype 3 cultured on 0.5 mg l(-1) BAP. Mean number of shoots per explant varied from one to five. The ability of black willow inflorescences to produce adventitious shoots makes them potential targets for Agrobacterium-mediated transformation with heavy-metal-resistant genes for phytoremediation.

  4. Use of willows in evapotranspirative systems for onsite wastewater management – theory and experiences from Denmark

    DEFF Research Database (Denmark)

    Brix, Hans; Arias, Carlos Alberto

    2011-01-01

    aspects of ET systems is their ability to evapotranspire all of the sewage discharged into the systems and the rain falling onto the systems. On an annual basis the ET should equal the amount of wastewater discharged into the system plus the amount of precipitation falling onto the system. Part...... with clones of willow (Salix viminalis L.). The surface area of the systems depends on the amount and quality of the sewage to be treated and the local annual rainfall. A single household in Denmark typically requires between 120 and 300 m2. The annual precipitation at the site of construction is an important...... dimensioning parameter. Settled sewage is dispersed underground into the bed under pressure. The stems of the willows are harvested on a regular basis to stimulate the growth of the willows and to remove some nutrients and heavy metals. In this paper, the theory behind the operation of willow based ET systems...

  5. Test of aerobic TCE degradation by willows (Salix viminalis) and willows inoculated with TCE-cometabolizing strains of Burkholderia cepacia

    DEFF Research Database (Denmark)

    Clausen, Lauge Peter Westergaard; Broholm, Mette Martina; Gosewinkel, Ulrich Bay

    2017-01-01

    . cepacia (301C, PR1-31 and VM1330-pTOM), using chloride formation as an indicator of dehalogenation. Willows were grown in non-sterile, hydroponic conditions for 3 weeks in chloride-free nutrient solution spiked with TCE. TCE was added weekly due to rapid loss by volatilization. Chloride and TCE...

  6. Wolf presence and increased willow consumption by Yellowstone elk: implications for trophic cascades.

    Science.gov (United States)

    Creel, Scott; Christianson, David

    2009-09-01

    Recent increases in the height and growth ring width of willow (Salix spp.) and other woody plants in the Greater Yellowstone Ecosystem (GYE) have been attributed to a behaviorally mediated trophic cascade from wolves (Canis lupus) to elk (Cervus elaphus) to willows. This hypothesis predicts that individual elk consume less willow in response to the presence of wolves, but this prediction has not been directly tested with data from elk. We collected 727 fecal samples from elk in the Gallatin Canyon portion of the GYE over three winters and used microhistological methods to quantify the proportion of willow in each sample. We then tested the effect of wolf presence on willow consumption by elk, controlling for the effects of snow conditions, sex, and habitat type. During the period of study, 8-17 wolves occupied the study area, and wolves were locally present on 49% of 260 sampling days, stratified at two-week intervals across three drainages. Over the three years combined, willow consumption was related to snow conditions, wolf presence, and a wolf X sex interaction. As expected, willow consumption increased with deeper and less penetrable snow, and this effect was strong. Contrary to expectation, willow consumption increased in the presence of wolves. As with other aspects of antipredator behavior, wolves had different effects on willow consumption by males and females. Finally, we aggregated the data to estimate winter-long mean willow consumption within each drainage; at this broader scale, willow consumption again increased as predation risk increased. In summary, willow consumption was more strongly affected by snow conditions than by the presence of wolves. Interactions between elk and willow were affected by wolves, but not as predicted by the hypothesis that wolf presence favors willow release through a reduction in the selection of willow by individual elk. If a trophic cascade is operating, our results suggest that a decline in the size of the elk

  7. Willow yield is highly dependent on clone and site

    DEFF Research Database (Denmark)

    Ugilt Larsen, Søren; Jørgensen, Uffe; Lærke, Poul Erik

    2014-01-01

    Use of high-yielding genotypes is one of the means to achieve high yield and profitability in willow (Salix spp.) short rotation coppice. This study investigated the performance of eight willow clones (Inger, Klara, Linnea, Resolution, Stina, Terra Nova, Tora, Tordis) on five Danish sites......, differing considerably in soil type, climatic conditions and management. Compared to the best clone, the yield was up to 36 % lower for other clones across sites and up to 51 % lower within sites. Tordis was superior to other clones with dry matter yields between 5.2 and 10.2 Mg ha−1 year−1 during the first...... 3-year harvest rotation, and it consistently ranked as the highest yielding clone on four of the five sites and not significantly lower than the highest yielding clone on the fifth site. The ranking of the other clones was more dependent on site with significant interaction between clone and site...

  8. Water relations and gas exchange in poplar and willow under water stress and elevated atmospheric CO2.

    Science.gov (United States)

    Johnson, Jon D; Tognetti, Roberto; Paris, Piero

    2002-05-01

    Predictions of shifts in rainfall patterns as atmospheric [CO2] increases could impact the growth of fast growing trees such as Populus spp. and Salix spp. and the interaction between elevated CO2 and water stress in these species is unknown. The objectives of this study were to characterize the responses to elevated CO2 and water stress in these two species, and to determine if elevated CO2 mitigated drought stress effects. Gas exchange, water potential components, whole plant transpiration and growth response to soil drying and recovery were assessed in hybrid poplar (clone 53-246) and willow (Salix sagitta) rooted cuttings growing in either ambient (350 &mgr;mol mol-1) or elevated (700 &mgr;mol mol-1) atmospheric CO2 concentration ([CO2]). Predawn water potential decreased with increasing water stress while midday water potentials remained unchanged (isohydric response). Turgor potentials at both predawn and midday increased in elevated [CO2], indicative of osmotic adjustment. Gas exchange was reduced by water stress while elevated [CO2] increased photosynthetic rates, reduced leaf conductance and nearly doubled instantaneous transpiration efficiency in both species. Dark respiration decreased in elevated [CO2] and water stress reduced Rd in the trees growing in ambient [CO2]. Willow had 56% lower whole plant hydraulic conductivity than poplar, and showed a 14% increase in elevated [CO2] while poplar was unresponsive. The physiological responses exhibited by poplar and willow to elevated [CO2] and water stress, singly, suggest that these species respond like other tree species. The interaction of [CO2] and water stress suggests that elevated [CO2] did mitigate the effects of water stress in willow, but not in poplar.

  9. Railway transport and terminal transhipment of Short Rotation Willow Crops. Pre-study; Taagtransport och terminal-hantering av Salix. Foerstudie

    Energy Technology Data Exchange (ETDEWEB)

    Forsberg, Maya

    2009-07-01

    The overall aim of this pre-study is to contribute to an increased co-handling of biomass from forest and field at terminal and railway transport, in order to find effective transport solutions with regard to costs and environment. The aim of the pre-study is also to get an overall picture of transport of biomass on railway. The objective is to draw conclusions of use for interested parties within the agricultural sector, on what possibilities there are to handle biomass from forestry and agriculture with the same logistical infrastructure. Conclusions are drawn especially with regard to Short Rotation Willow Crops (here Salix) handled as chips or bundles, but also briefly for other energy crops suitable for combustion. Furthermore, the study draws conclusions on how to design effective terminals and related costs. The pre-study is intended as a planning material for a more in-depth study of a mobile terminal solution, with active participation from interested players in the agricultural sector. When considering railway transport and terminal transhipment of large volumes of biomass, there is a clear economical benefit in designing large terminals and making investments in effective handling equipment, in order to get a high use of equipment and a high storage turnover. A more simple and temporary terminal solution may be suitable for smaller flows of biomass, such as agricultural fuels, which need to be collected in different areas according to availability. An intermodal solution, i.e. which involves the transportation of freight in the same type of carrier (a container for example) when changing modes of transportation, is suitable for this purpose. The solution should also build on standardised equipment, involve low terminal investments and flexible and temporary transhipment in different areas near the location of the biomass. For railway transport, the same handling equipment may be used for wood chips and Salix chips, i.e. standardised container systems

  10. Willow clones with high biomass yield in short rotation coppice in the southern region of Tohoku district (Japan)

    International Nuclear Information System (INIS)

    Mitsui, Yu; Seto, Shoko; Nishio, Mari; Minato, Kazuya; Ishizawa, Kimiharu; Satoh, Shigeru

    2010-01-01

    The present study was conducted to select willow (Salix spp.) clones with a high potential for use as biomass energy crops in the southern region of Tohoku district in Japan. Cuttings of 8 willow clones were planted on an abandoned farmland near Sendai (av. annual temp., 10.9 o C) in March 2006, grown throughout the year and cut back in late December 2006 to resprout from the remaining stools in March 2007. The biomass yield in December 2007, after the first growing season, was highest in Salix pet-susu clone KKD, followed by Salix pseudolinearis clone FXM and Salix sachalinensis clone SEN. The biomass yield on December 2008, after the second growing season, was again highest in clone KKD followed by clone FXM, S. pet-susu clone HB471 and S. sachalinensis clone SEN; the average annual yield of dry mass after the second growing season being 3.09, 2.58, 2.17 and 1.85 kgDM plant -1 for the clones in this order. Plant growth form differed among the clones. Clones FXM and SEN had several shoots of almost uniform base diameter, whereas clones KKD and HB471 showed plagiotropic growth with one thick and several thin shoots. The calorific values of dried stem segments were similar among clones, ranging from 18.7 to 19.1 kJ g -1 . The dried stem segments contained 78.9-81.2 wt.% hollocellulose, 27.2-32.3 wt.% lignin and 2.1-4.0 wt.% extractives with ethanol-benzene, depending on clones. Based on these results, we could select four clones (KKD, FXM, HB471 and SEN) suitable for biomass production by SRWC in this area.

  11. The effect of pH on the uptake and toxicity of the bivalent weak base chloroquine tested on Salix viminalis and Daphnia magna

    DEFF Research Database (Denmark)

    Rendal, Cecilie; Kusk, Kresten Ole; Trapp, Stefan

    2011-01-01

    , and therefore a higher toxicity can be expected. The current study examines the pHdependent toxicity and bioaccumulation of the bivalent weak base chloroquine (pKa: 10.47 and 6.33, log KOW 4.67) tested on Salix viminalis (basket willow) and Daphnia magna (water flea). The transpiration rates of hydroponically...

  12. Screening of willow species for resistance to heavy metals: comparison of performance in a hydroponics system and field trials.

    Science.gov (United States)

    Watson, C; Pulford, I D; Riddell-Black, D

    2003-01-01

    The aim of this study was to ascertain whether metal resistance in willow (Salix) clones grown in a hydroponics screening test correlated with data from the same clones grown independently in a field trial. If so, results from a short-term, glasshouse-based system could be extrapolated to the field, allowing rapid identification of willows suitable for planting in metal-contaminated substrates without necessitating longterm field trials. Principal Components Analysis was used to show groups of clones and to assess the relative importance of the parameters measured in both the hydroponics system and the field; including plant response factors such as increase in stem height, as well as metal concentrations in plant tissues. The clones tested fell into two distinct groups. Salix viminalis clones and the basket willow Black Maul (S. triandra) were less resistant to elevated concentrations of heavy metals than a group of hardier clones, including S. burjatica 'Germany,' S.x dasyclados, S. candida and S. spaethii. The more resistant clones produced more biomass in the glasshouse and field, and had higher metal concentrations in the wood. The less resistant clones had greater concentrations of Cu and Ni in the bark, and produced less biomass in the glasshouse and field. Significant relationships were found between the response of the same clones grown the in short-term glasshouse hydroponics system and in the field.

  13. A comparison of the suitability of different willow varieties to treat on-site wastewater effluent in an Irish climate.

    Science.gov (United States)

    Curneen, S J; Gill, L W

    2014-01-15

    Short rotation coppiced willow trees can be used to treat on-site wastewater effluent with the advantage that, if planted in a sealed basin and sized correctly, they produce no effluent discharge. This paper has investigated the evapotranspiration rate of four different willow varieties while also monitoring the effects of three different effluent types on each variety. The willow varieties used are all cultivars of Salix viminalis. The effluents applied were primary (septic tank) effluent, secondary treated effluent and rain water (control). The results obtained showed that the addition of effluent had a positive effect on the evapotranspiration. The willows were also found to uptake a high proportion of the nitrogen and phosphorus from the primary and secondary treated effluents added during the first year. The effect of the different effluents on the evapotranspiration rate has been used to design ten full scale on-site treatment systems which are now being monitored. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. Variation in copper and zinc tolerance and accumulation in 12 willow clones: implications for phytoextraction*

    Science.gov (United States)

    Yang, Wei-dong; Wang, Yu-yan; Zhao, Feng-liang; Ding, Zhe-li; Zhang, Xin-cheng; Zhu, Zhi-qiang; Yang, Xiao-e

    2014-01-01

    Willows (Salix spp.) have shown high potential for the phytoextraction of heavy metals. This study compares variations in copper (Cu) and zinc (Zn) tolerance and accumulation potential among 12 willow clones grown in a nutrient solution treated with 50 μmol/L of Cu or Zn, respectively. The results showed differences in the tolerance and accumulation of Cu and Zn with respect to different species/clones. The biomass variation among clones in response to Cu or Zn exposure ranged from the stimulation of growth to inhibition, and all of the clones tested showed higher tolerance to Cu than to Zn. The clones exhibited less variation in Cu accumulation but larger variation in Zn accumulation. Based on translocation factors, it was found that most of the Cu was retained in the roots and that Zn was more mobile than Cu for all clones. It is concluded that most willow clones are good accumulators of Zn and Cu. PMID:25183033

  15. Uptake, accumulation and metabolic response of ferricyanide in weeping willows.

    Science.gov (United States)

    Yu, Xiao-Zhang; Gu, Ji-Dong

    2009-01-01

    The remediation potential and metabolic responses of plants to ferricyanide were investigated using pre-rooted weeping willows (Salix babylonica L.) grown hydroponically in growth chambers and treated with potassium ferricyanide. Positive responses were observed for the plants exposed to cyanide recovered in plant biomass was constant in all treatments, indicating that transport is a major limiting step for the uptake of ferricyanide by plants. The majority of the ferricyanide taken up from the growth media was possibly assimilated during transport through plants. The velocity of the removal processes can be described by Michaelis-Menten kinetics, and the half-saturation constant (K(M)) and the maximum removal capacity (v(max)) were estimated to be 228.1 mg CN L(-1) and 36.43 mg CN kg(-1) d(-1), respectively, using non-linear regression methods. These results suggest that weeping willows can take up, transport and assimilate ferricyanide; and phytoremediation is an option for cleaning up the environmental sites contaminated with cyanide complexes.

  16. Willow on Yellowstone's northern range: evidence for a trophic cascade?

    Science.gov (United States)

    Beyer, Hawthorne L; Merrill, Evelyn H; Varley, Nathan; Boyce, Mark S

    2007-09-01

    Reintroduction of wolves (Canis lupus) to Yellowstone National Park in 1995-1996 has been argued to promote a trophic cascade by altering elk (Cervus elaphus) density, habitat-selection patterns, and behavior that, in turn, could lead to changes within the plant communities used by elk. We sampled two species of willow (Salix boothii and S. geyeriana) on the northern winter range to determine whether (1) there was quantitative evidence of increased willow growth following wolf reintroduction, (2) browsing by elk affected willow growth, and (3) any increase in growth observed was greater than that expected by climatic and hydrological factors alone, thereby indicating a trophic cascade caused by wolves. Using stem sectioning techniques to quantify historical growth patterns we found an approximately twofold increase in stem growth-ring area following wolf reintroduction for both species of willow. This increase could not be explained by climate and hydrological factors alone; the presence of wolves on the landscape was a significant predictor of stem growth above and beyond these abiotic factors. Growth-ring area was positively correlated with the previous year's ring area and negatively correlated with the percentage of twigs browsed from the stem during the winter preceding growth, indicating that elk browse impeded stem growth. Our results are consistent with the hypothesis of a behaviorally mediated trophic cascade on Yellowstone's northern winter range following wolf reintroduction. We suggest that the community-altering effects of wolf restoration are an endorsement of ecological-process management in Yellowstone National Park.

  17. Irrigation water quality influences heavy metal uptake by willows in biosolids.

    Science.gov (United States)

    Laidlaw, W Scott; Baker, Alan J M; Gregory, David; Arndt, Stefan K

    2015-05-15

    Phytoextraction is an effective method to remediate heavy metal contaminated landscapes but is often applied for single metal contaminants. Plants used for phytoextraction may not always be able to grow in drier environments without irrigation. This study investigated if willows (Salix x reichardtii A. Kerner) can be used for phytoextraction of multiple metals in biosolids, an end-product of the wastewater treatment process, and if irrigation with reclaimed and freshwater influences the extraction process. A plantation of willows was established directly onto a tilled stockpile of metal-contaminated biosolids and irrigated with slightly saline reclaimed water (EC ∼2 dS/cm) at a wastewater processing plant in Victoria, Australia. Biomass was harvested annually and analysed for heavy metal content. Phytoextraction of cadmium, copper, nickel and zinc was benchmarked against freshwater irrigated willows. The minimum irrigation rate of 700 mm per growing season was sufficient for willows to grow and extract metals. Increasing irrigation rates produced no differences in total biomass and also no differences in the extraction of heavy metals. The reclaimed water reduced both the salinity and the acidity of the biosolids significantly within the first 12 months after irrigation commenced and after three seasons the salinity of the biosolids had dropped to metal extraction. Reclaimed water irrigation reduced the biosolid pH and this was associated with reductions of the extraction of Ni and Zn, it did not influence the extraction of Cu and enhanced the phytoextraction of Cd, which was probably related to the high chloride content of the reclaimed water. Our results demonstrate that flood-irrigation with reclaimed water was a successful treatment to grow willows in a dry climate. However, the reclaimed water can also change biosolids properties, which will influence the effectiveness of willows to extract different metals. Copyright © 2015 Elsevier Ltd. All rights

  18. Harvest and logistics for better profitability from small cultivations of Short Rotation Willow Coppice; Skoerdeteknik och logistik foer baettre loensamhet fraan smaa odlingar av Salix

    Energy Technology Data Exchange (ETDEWEB)

    Baky, Andras; Forsberg, Maya; Rosenqvist, Haakan; Jonsson, Nils; Sundberg, Martin

    2010-06-15

    In Sweden, the political desire to increase the amount of short rotation willow coppice (Salix) plantations has been expressed. However, for various reasons interest from farmers has been low. The hypothesis of this study is that the total area of Salix cultivation can be increased by also cultivating fields smaller than those generally considered economic today. In order to lower production costs, machine systems adapted for harvest of smaller fields are required. The possibility of using farmers' existing tractors and more convenient machines, as well as achieving lower machine costs for smaller fields, may increase farmers' interest. The long-term objective is to achieve large-scale deliveries of willow with small-scale solutions at farm level, as an option and complement to today's more large-scale systems for harvesting willow. Costs, energy use and climatic impact (CO{sub 2} emissions) for two harvest and logistical chains suitable for small fields have been calculated from field to energy plant, and methods for minimizing these costs have been analyzed. Comparison is made with the direct chipping system, the most commonly used in Sweden today. The systems studied comprised: 1. Direct bundling harvest system with a tractor-towed harvester, collection of bundles in the field with a trailer-mounted crane, and storage in a pile before delivery. Chipping is performed at the energy plant. 2. Direct billeting with a tractor-towed harvester accompanied simultaneously by a tractor and trailer for collection, and storage in a pile before delivery. 3. Direct chipping with a self-propelled modified forage harvester accompanied simultaneously by a tractor and container for collection, and direct delivery to plant. Both the billet and bundle systems show higher costs than the direct chipping system, irrespective of field size. The analysis of different scenarios and conditions shows possibilities of lowering the costs through certain measures. Furthermore

  19. Environmental effects of energy forest (short rotation willow)

    International Nuclear Information System (INIS)

    Frank, K.

    1994-01-01

    This paper deals with environmental effects of producing and combusting energy forest fuel. Energy forest means short rotation willow (Salix). Supposed effects of sewage sludge application are discussed as well. Energy forestry on agricultural land implies both advantages and disadvantages for the environment. Leaf litter (humified leaves) increases the humus content in the top soil. The soil fauna is also positively affected. Until now performed studies about nitrogen leakage from fields cultivated with energy forest (Salix), have not given any distinct results. A retarded drainage within energy forest fields is on the other hand noticed. While the root system of Salix is active during a long period of the year, the nitrogen leakage become less compared to traditional cultivation. The content of plant nutrients and organic matter in sewage sludge make a resource that can be useful for agricultural purposes, especially for energy forest cultivation. The content of heavy metals and organic emissions contradicts sludge application to agricultural land. Sewage sludge with todays quality increases somewhat the content of heavy metals in the soil. This condition can be counteracted to a certain extent by growing energy forest. It has been established that Salix has high ability to heavy metal uptake, especially cadmium. Growing energy forest on drained farm land is connected with a risk for root penetration into the drainage system. With enough water and plant nutrients in the top soil the risk is reduced. Shallow depth of the pipes increases the risk. Combustion of wood chip from energy forests (and other types of biomass) gives especially two advantages. It does not give any net contribution of carbon dioxide to the atmosphere. The sulphur discharge will be minimal since the sulphur content in wood fuels is low. Discharge of nitrogen oxide and hydrocarbons may give some problems. These can be reduced by technological measures when combusting. 27 refs, 4 tabs

  20. Phytoremediation capacity of poplar (Populus spp. and willow (Salix spp. clonesin relation to photosynthesis

    Directory of Open Access Journals (Sweden)

    Pajević Slobodanka

    2009-01-01

    Full Text Available Good photosynthetic features and a favorable water regimes of woody plants improve their survival and remediation potential under unfavorable ecological conditions. Accordingly, we here present results of testing plant tolerance of Pb, Cd, Ni, and diesel fuel based on gas exchange parameters and WUE of four poplar and two willow clones grown in a greenhouse on soil culture. Photosynthesis and transpiration of plants grown on soils with individually applied heavy metals decreased significantly, but this was less obvious in the case of Cd treatment. A heavy metal mixture in the soil induced significant reduction in photosynthesis (by more than 50%. Diesel fuel as the only pollutant in soil caused very strong and significant inhibition of photosynthesis and transpiration of willow clones. The results indicate genotypic specificity of all investigated physiological parameters and mark poplar clones as very useful in phytoextraction technology for the bio-cleaning of chemically polluted soils.

  1. Wastewater purification in a willow plantation. The case study at Aarike

    International Nuclear Information System (INIS)

    Kuusemets, V.; Mauring, T.

    1996-01-01

    In order to combine wastewater purification and biomass production for energy purposes, a willow plantation for wastewater treatment was established in 1995 in Aarike, Southern Estonia. Wastewater from a dwelling house (25 person equivalents, pe) is treated in a combined free-water filter system consisting of three separate basins, isolated with clay and having filter beds of gravel and sand mixture. The beds were planted with Salix viminalis. At the end of the first growing season, the purification efficiency of the newly established treatment system was 65% for BOD 7 , 43% for nitrogen and 11% for phosphorus removal. At the end of the establishment year, the above ground production of willow stems (bark and wood) and leaves was 1.3 and 0.3 t ha -1 , respectively. The figures are about three to five times higher than those recorded in previously established energy forest plantations of comparable ages in Estonia. 15 refs, 2 figs

  2. UTILIZATION OF ANIMAL MEAL FOR GROWING OF WILLOW DEDICATED FOR ENERGY

    Directory of Open Access Journals (Sweden)

    Anna Nogalska

    2016-09-01

    Full Text Available The aim of the study was to evaluate the effects of increased rates of meat and bone meal (MBM to the soil on biomass yield, nitrogen (N and phosphorus (P content, and their accumulation in above ground biomass Salix viminalis, as well as the content of mineral N and available P forms in the soil. A two-year pot experiment was conducted at Warmia and Mazury University in Olsztyn (north-eastern Poland. Average biomass yield from two seasons of the studies was three higher from MBM treatments comparing to unfertilized control. Higher rates of MBM particularly in the second year showed higher yielding potential compared with mineral fertilizers. Willow biomass harvested from MBM treatments generally showed lower content of studied nutrients than willow from control treatment. It was found that in relation to the control accumulation of N in willow above ground biomass was significantly lower for 0.5% MBM treatment and significantly higher for the treatment with the highest rate (2.0%. The higher accumulation of P was found in the second year after MBM application, except treatment with the lowest MBM rate. Correlation coefficients values indicated that there is a relation between MBM rate and content of mineral N and available P in soil.

  3. Cytospora species from Populus and Salix in China with C. davidiana sp. nov.

    Science.gov (United States)

    Wang, Yan-Li; Lu, Quan; Decock, Cony; Li, Yong-Xia; Zhang, Xing-Yao

    2015-05-01

    Poplar and willow plantations have become widespread in China, in order to meet national economic and environmental needs. The emergence of several pathogens is enhanced by climatic change and associated human factors. Species of Cytospora are well-known pathogens on poplar and willow, and cause stem cankers and diebacks. In the present study, we conducted a survey of Cytospora species occurring on Populus spp. and Salix spp. in China. We used morphological examination and phylogenetic inferences, based on the DNA sequence data from the internal transcribed spacer regions (ITS1, 5.8S rDNA, and ITS2) and partial β-tubulin gene, to identify six Cytospora species occurring on poplar and willow. Five of these species belonged to known taxa, viz. Cytospora chrysosperma (asexual state of Valsa sordida), Cytospora translucens (asexual state of Leucostoma translucens), Cytospora fugax (asexual state of Valsa salicina), Cytospora atrocirrhata, and Cytospora kantschavelii. Our study yielded a new species, Cytospora davidiana sp. nov., on poplar. The new species is characterized by typical torsellioid conidiomata. An additional Cytospora sp. 1, which formed a distinct clade in the phylogenetic inferences, remains unnamed; the paucity of available materials prevented phenotypical characterization. Copyright © 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  4. The development of short-rotation willow in the northeastern United States for bioenergy and bioproducts, agroforestry and phytoremediation

    International Nuclear Information System (INIS)

    Volk, T.A.; Abrahamson, L.P.; Nowak, C.A.; White, E.H.; Smart, L.B.; Tharakan, P.J.

    2006-01-01

    Research on willow (Salix spp.) as a locally produced, renewable feedstock for bioenergy and bioproducts began in New York in the mid-1980s in response to growing concerns about environmental impacts associated with fossil fuels and declining rural economies. Simultaneous and integrated activities-including research, large-scale demonstrations, outreach and education, and market development-were initiated in the mid-1990s to facilitate the commercialization of willow biomass crops. Despite technological viability and associated environmental and local economic benefits, the high price of willow biomass relative to coal has been a barrier to wide-scale deployment of this system. The cost of willow biomass is currently $3.00GJ -1 ($57.30odt -1 ) compared to $1.40-1.90GJ -1 for coal. Yield improvements from traditional breeding efforts and increases in harvesting efficiency that are currently being realized promise to reduce the price differential. Recent policy changes at the federal level, including the provision to harvest bioenergy crops from Conservation Reserve Program (CRP) land and a closed-loop biomass tax credit, and state-level initiatives such as Renewable Portfolio Standards (RPS) will help to further reduce the difference and foster markets for willow biomass. Years of work on willow biomass crop research and demonstration projects have increased our understanding of the biology, ecophysiology and management of willow biomass crops. Using an adaptive management model, this information has led to the deployment of willow for other applications such as phytoremediation, living snow fences, and riparian buffers across the northeastern US. (author)

  5. Production capacity of biomass of the floodpain community of Salix alba L. in southern Moravia

    Directory of Open Access Journals (Sweden)

    Diana López

    2007-01-01

    Full Text Available The paper deals with the study of the production capacity of biomass in the seven-year stand of Salix alba L. The communities originated in the process of primary succession in the area of the middle Nové Mlýny reservoir on a newly established island. Already since the first stages, the communities have been monitored. Results have shown that white willow behaves as an R-strategist with fast growth in youth. Moreover, the growth is supported by optimum environmental conditions (soils richly supplied with nutrients and water, long growing season. Accumulated phytomass amounted 102,7 t.ha−1 at the age of 7 years and the yield reached a mean annual increment of 15 t.ha−1.year−1. Communities of white willow rank among highly productive phytocoenoses capable of fixing considerable amounts of carbon and, at the same time fulfilling the function of habitat corridors.

  6. Simulation modeling to understand how selective foraging by beaver can drive the structure and function of a willow community

    Science.gov (United States)

    Peinetti, H.R.; Baker, B.W.; Coughenour, M.B.

    2009-01-01

    Beaver-willow (Castor-Salix) communities are a unique and vital component of healthy wetlands throughout the Holarctic region. Beaver selectively forage willow to provide fresh food, stored winter food, and construction material. The effects of this complex foraging behavior on the structure and function of willow communities is poorly understood. Simulation modeling may help ecologists understand these complex interactions. In this study, a modified version of the SAVANNA ecosystem model was developed to better understand how beaver foraging affects the structure and function of a willow community in a simulated riparian ecosystem in Rocky Mountain National Park, Colorado (RMNP). The model represents willow in terms of plant and stem dynamics and beaver foraging in terms of the quantity and quality of stems cut to meet the energetic and life history requirements of beaver. Given a site where all stems were equally available, the model suggested a simulated beaver family of 2 adults, 2 yearlings, and 2 kits required a minimum of 4 ha of willow (containing about10 stems m-2) to persist in a steady-state condition. Beaver created a willow community where the annual net primary productivity (ANPP) was 2 times higher and plant architecture was more diverse than the willow community without beaver. Beaver foraging created a plant architecture dominated by medium size willow plants, which likely explains how beaver can increase ANPP. Long-term simulations suggested that woody biomass stabilized at similar values even though availability differed greatly at initial condition. Simulations also suggested that willow ANPP increased across a range of beaver densities until beaver became food limited. Thus, selective foraging by beaver increased productivity, decreased biomass, and increased structural heterogeneity in a simulated willow community.

  7. Phytoremediation of Metal Contaminated Soil Using Willow: Exploiting Plant-Associated Bacteria to Improve Biomass Production and Metal Uptake.

    Science.gov (United States)

    Janssen, Jolien; Weyens, Nele; Croes, Sarah; Beckers, Bram; Meiresonne, Linda; Van Peteghem, Pierre; Carleer, Robert; Vangronsveld, Jaco

    2015-01-01

    Short rotation coppice (SRC) of willow and poplar is proposed for economic valorization and concurrently as remediation strategy for metal contaminated land in northeast-Belgium. However, metal phytoextraction appears insufficient to effectuate rapid reduction of soil metal contents. To increase both biomass production and metal accumulation of SRC, two strategies are proposed: (i) in situ selection of the best performing clones and (ii) bioaugmentation of these clones with beneficial plant-associated bacteria. Based on field data, two experimental willow clones, a Salix viminalis and a Salix alba x alba clone, were selected. Compared to the best performing commercial clones, considerable increases in stem metal extraction were achieved (up to 74% for Cd and 91% for Zn). From the selected clones, plant-associated bacteria were isolated and identified. All strains were subsequently screened for their plant growth-promoting and metal uptake enhancing traits. Five strains were selected for a greenhouse inoculation experiment with the selected clones planted in Cd-Zn-Pb contaminated soil. Extraction potential tended to increase after inoculation of S. viminalis plants with a Rahnella sp. strain due to a significantly increased twig biomass. However, although bacterial strains showing beneficial traits in vitro were used for inoculation, increments in extraction potential were not always observed.

  8. Resprout and survival of willows (Salix purpurea and S. incana), Poplars (Populus nigra) and Tamaris (Tamarix gallica) cuttings in marly gullies with Southern aspect in a mountainous and Mediterranean climate (Southern Alps, France)

    Science.gov (United States)

    Rey, Freddy; Labonne, Sophie; Dangla, Laure; Lavandier, Géraud

    2014-05-01

    In the Southern French Alps under a mountainous and Mediterranean climate, a current strategy of bioengineering is developed for trapping sediment in marly gullies with surface area less than 1 ha. It is based on the use of structures in the form of brush layers and brush mats of cuttings on deadwood microdams. Purple and white Willows (Salix purpurea and S. incana) are recommended here as they proved their efficiency to resprout and survive in such environment. However, these species installed in Southern gullies did not survive in previous experiments, due to the too harsh conditions of solar radiation and drought. We thus decided to test other species, namely black Poplar (Populus nigra) and Tamaris (Tamarix gallica), which proved their resistance to drought conditions in other experiments. To this view, bioengineering structures have been built in 2010 in eroded marly gullies in the Roubines and Fontaugier catchments (Southern Alps, France). We tested two installation modalities: one in spring and a second in autumn. Seventy-eight bioengineering structures (50 in spring and 28 in autumn), among which 32 made with Poplar cuttings and 28 with Tamaris cuttings, as well as 11 structures with purple Willow and 7 with white Willow as controls, were built in 6 experimental gullies. After 3 observation years for each modality (2010 to 2012, and 2011 to 2013, respectively), results first revealed that Willow species succeeded in surviving in gullies in Southern aspect (76 % for the cuttings installed in spring and 52 % for those installed in autumn), which is in contradiction with previous results. Second, Poplar showed a good ability to survive (62 % for the cuttings installed in spring and 33 % for those installed in autumn). Tamaris obtained the worst score with 26 % and 38 % of survival for the cuttings installed in spring and autumn, respectively. Globally, excepted for Tamaris, survival rates were better for the cuttings installed in spring. The bioengineering

  9. COMPACTION STUDIES OF TORREFIED WILLOW

    Directory of Open Access Journals (Sweden)

    Michał Rejdak

    2017-01-01

    Full Text Available The article presents the results of studies of torrefied willow (Salix viminalis L. compaction. Densification tests were performed using a hydraulic press with a maximum pressure of 216 MPa. The effect of basic parameters of the briquetting process (pressure and temperature on mechanical parameters of manufactured briquettes were determined. On the basis of the research, it was found that the increase in pressure and temperature of the densification process increases the density and strength of pressed briquettes. The positive effect of temperature is particularly noticeable at lower pressing pressures (36 MPa – 72 MPa. In the case of a temperature of 300 °C, the increase in a pressure from 144 MPa to 216 MPa resulted in the decrease in the density and strength of the briquette. It was also found that the briquettes manufactured at this temperature are characterized by lower density and strength than the briquettes obtained at a temperature of 200 °C.

  10. Variations in metal tolerance and accumulation in three hydroponically cultivated varieties of Salix integra treated with lead.

    Directory of Open Access Journals (Sweden)

    Shufeng Wang

    Full Text Available Willow species have been suggested for use in the remediation of contaminated soils due to their high biomass production, fast growth, and high accumulation of heavy metals. The tolerance and accumulation of metals may vary among willow species and varieties, and the assessment of this variability is vital for selecting willow species/varieties for phytoremediation applications. Here, we examined the variations in lead (Pb tolerance and accumulation of three cultivated varieties of Salix integra (Weishanhu, Yizhibi and Dahongtou, a shrub willow native to northeastern China, using hydroponic culture in a greenhouse. In general, the tolerance and accumulation of Pb varied among the three willow varieties depending on the Pb concentration. All three varieties had a high tolerance index (TI and EC50 value (the effective concentration of Pb in the nutrient solution that caused a 50% inhibition on biomass production, but a low translocation factor (TF, indicating that Pb sequestration is mainly restricted in the roots of S. integra. Among the three varieties, Dahogntou was more sensitive to the increased Pb concentration than the other two varieties, with the lowest EC50 and TI for root and above-ground tissues. In this respect, Weishanhu and Yizhibi were more suitable for phytostabilization of Pb-contaminated soils. However, our findings also indicated the importance of considering the toxicity symptoms when selecting willow varieties for the use of phytoremediation, since we also found that the three varieties revealed various toxicity symptoms of leaf wilting, chlorosis and inhibition of shoot and root growth under the higher Pb concentrations. Such symptoms could be considered as a supplementary index in screening tests.

  11. Variations in Metal Tolerance and Accumulation in Three Hydroponically Cultivated Varieties of Salix integra Treated with Lead

    Science.gov (United States)

    Sun, Haijing; Chen, Yitai; Pan, Hongwei; Yang, Xiaoe; Rafiq, Tariq

    2014-01-01

    Willow species have been suggested for use in the remediation of contaminated soils due to their high biomass production, fast growth, and high accumulation of heavy metals. The tolerance and accumulation of metals may vary among willow species and varieties, and the assessment of this variability is vital for selecting willow species/varieties for phytoremediation applications. Here, we examined the variations in lead (Pb) tolerance and accumulation of three cultivated varieties of Salix integra (Weishanhu, Yizhibi and Dahongtou), a shrub willow native to northeastern China, using hydroponic culture in a greenhouse. In general, the tolerance and accumulation of Pb varied among the three willow varieties depending on the Pb concentration. All three varieties had a high tolerance index (TI) and EC50 value (the effective concentration of Pb in the nutrient solution that caused a 50% inhibition on biomass production), but a low translocation factor (TF), indicating that Pb sequestration is mainly restricted in the roots of S. integra. Among the three varieties, Dahogntou was more sensitive to the increased Pb concentration than the other two varieties, with the lowest EC50 and TI for root and above-ground tissues. In this respect, Weishanhu and Yizhibi were more suitable for phytostabilization of Pb-contaminated soils. However, our findings also indicated the importance of considering the toxicity symptoms when selecting willow varieties for the use of phytoremediation, since we also found that the three varieties revealed various toxicity symptoms of leaf wilting, chlorosis and inhibition of shoot and root growth under the higher Pb concentrations. Such symptoms could be considered as a supplementary index in screening tests. PMID:25268840

  12. Assembly and comparative analysis of complete mitochondrial genome sequence of an economic plant Salix suchowensis

    Directory of Open Access Journals (Sweden)

    Ning Ye

    2017-03-01

    Full Text Available Willow is a widely used dioecious woody plant of Salicaceae family in China. Due to their high biomass yields, willows are promising sources for bioenergy crops. In this study, we assembled the complete mitochondrial (mt genome sequence of S. suchowensis with the length of 644,437 bp using Roche-454 GS FLX Titanium sequencing technologies. Base composition of the S. suchowensis mt genome is A (27.43%, T (27.59%, C (22.34%, and G (22.64%, which shows a prevalent GC content with that of other angiosperms. This long circular mt genome encodes 58 unique genes (32 protein-coding genes, 23 tRNA genes and 3 rRNA genes, and 9 of the 32 protein-coding genes contain 17 introns. Through the phylogenetic analysis of 35 species based on 23 protein-coding genes, it is supported that Salix as a sister to Populus. With the detailed phylogenetic information and the identification of phylogenetic position, some ribosomal protein genes and succinate dehydrogenase genes are found usually lost during evolution. As a native shrub willow species, this worthwhile research of S. suchowensis mt genome will provide more desirable information for better understanding the genomic breeding and missing pieces of sex determination evolution in the future.

  13. Genome-wide identification and characterization of WRKY gene family in Salix suchowensis.

    Science.gov (United States)

    Bi, Changwei; Xu, Yiqing; Ye, Qiaolin; Yin, Tongming; Ye, Ning

    2016-01-01

    WRKY proteins are the zinc finger transcription factors that were first identified in plants. They can specifically interact with the W-box, which can be found in the promoter region of a large number of plant target genes, to regulate the expressions of downstream target genes. They also participate in diverse physiological and growing processes in plants. Prior to this study, a plenty of WRKY genes have been identified and characterized in herbaceous species, but there is no large-scale study of WRKY genes in willow. With the whole genome sequencing of Salix suchowensis, we have the opportunity to conduct the genome-wide research for willow WRKY gene family. In this study, we identified 85 WRKY genes in the willow genome and renamed them from SsWRKY1 to SsWRKY85 on the basis of their specific distributions on chromosomes. Due to their diverse structural features, the 85 willow WRKY genes could be further classified into three main groups (group I-III), with five subgroups (IIa-IIe) in group II. With the multiple sequence alignment and the manual search, we found three variations of the WRKYGQK heptapeptide: WRKYGRK, WKKYGQK and WRKYGKK, and four variations of the normal zinc finger motif, which might execute some new biological functions. In addition, the SsWRKY genes from the same subgroup share the similar exon-intron structures and conserved motif domains. Further studies of SsWRKY genes revealed that segmental duplication events (SDs) played a more prominent role in the expansion of SsWRKY genes. Distinct expression profiles of SsWRKY genes with RNA sequencing data revealed that diverse expression patterns among five tissues, including tender roots, young leaves, vegetative buds, non-lignified stems and barks. With the analyses of WRKY gene family in willow, it is not only beneficial to complete the functional and annotation information of WRKY genes family in woody plants, but also provide important references to investigate the expansion and evolution of

  14. Physiological and morphological responses of pine and willow saplings to post-fire salvage logging

    Science.gov (United States)

    Millions, E. L.; Letts, M. G.; Harvey, T.; Rood, S. B.

    2015-12-01

    With global warming, forest fires may be increasing in frequency, and post-fire salvage logging may become more common. The ecophysiological impacts of this practice on tree saplings remain poorly understood. In this study, we examined the physiological and morphological impacts of increased light intensity, due to post-fire salvage logging, on the conifer Pinus contorta (pine) and deciduous broadleaf Salix lucida (willow) tree and shrub species in the Crowsnest Pass region of southern Alberta. Photosynthetic gas-exchange and plant morphological measurements were taken throughout the summer of 2013 on approximately ten year-old saplings of both species. Neither species exhibited photoinhibition, but different strategies were observed to acclimate to increased light availability. Willow saplings were able to slightly elevate their light-saturated rate of net photosynthesis (Amax) when exposed to higher photosynthetic photon flux density (PPFD), thus increasing their growth rate. Willow also exhibited increased leaf inclination angles and leaf mass per unit area (LMA), to decrease light interception in the salvage-logged plot. By contrast, pine, which exhibited lower Amax and transpiration (E), but higher water-use efficiency (WUE = Amax/E) than willow, increased the rate at which electrons were moved through and away from the photosynthetic apparatus in order to avoid photoinhibition. Acclimation indices were higher in willow saplings, consistent with the hypothesis that species with short-lived foliage exhibit greater acclimation. LMA was higher in pine saplings growing in the logged plot, but whole-plant and branch-level morphological acclimation was limited and more consistent with a response to decreased competition in the logged plot, which had much lower stand density.

  15. Differential effects of glyphosate and aminomethylphosphonic acid (AMPA) on photosynthesis and chlorophyll metabolism in willow plants.

    Science.gov (United States)

    Gomes, Marcelo Pedrosa; Le Manac'h, Sarah Gingras; Maccario, Sophie; Labrecque, Michel; Lucotte, Marc; Juneau, Philippe

    2016-06-01

    We used a willow species (Salix miyabeana cultivar SX64) to examine the differential secondary-effects of glyphosate and aminomethylphosphonic acid (AMPA), the principal glyphosate by-product, on chlorophyll metabolism and photosynthesis. Willow plants were treated with different concentrations of glyphosate (equivalent to 0, 1.4, 2.1 and 2.8kgha(-1)) and AMPA (equivalent to 0, 0.28, 1.4 and 2.8kgha(-1)) and evaluations of pigment contents, chlorophyll fluorescence, and oxidative stress markers (hydrogen peroxide content and antioxidant enzyme activities) in leaves were performed after 12h of exposure. We observed that AMPA and glyphosate trigger different mechanisms leading to decreases in chlorophyll content and photosynthesis rates in willow plants. Both chemicals induced ROS accumulation in willow leaves although only glyphosate-induced oxidative damage through lipid peroxidation. By disturbing chlorophyll biosynthesis, AMPA induced decreases in chlorophyll contents, with consequent effects on photosynthesis. With glyphosate, ROS increases were higher than the ROS-sensitive threshold, provoking chlorophyll degradation (as seen by pheophytin accumulation) and invariable decreases in photosynthesis. Peroxide accumulation in both AMPA and glyphosate-treated plants was due to the inhibition of antioxidant enzyme activities. The different effects of glyphosate on chlorophyll contents and photosynthesis as described in the literature may be due to various glyphosate:AMPA ratios in those plants. Copyright © 2015 Elsevier Inc. All rights reserved.

  16. Cadmium in willow plantations - municipal waste products as fertilizers

    International Nuclear Information System (INIS)

    Hasselgren, K.

    1995-07-01

    Plantations of willows (i.e. Salix spp) seem to absorb cadmium (Cd) from arable land to a greater extent than other plant cultivations. Thus, energy forestry, based upon cultivation of Salix, could possibly be used for biofuel production in combination with biological purification of arable land contaminated with Cd by atmospheric deposition and P fertilizers during the passed 100 years. Application of sewage sludge (5-7 ppm Cd of dry solids) up to 550 g Cd per hectare, or 140 g Cd hectare and year, resulted in clearly increased concentrations of Cd in the upper 10 cm layer of the soil. In deeper soil layers the differences compared with control plots were small. Lower rates of sludge application, corresponding to less than 70 g added per hectare and year, did not materially affect the Cd-content in the soil irrespective of layer. A general pattern was that the content of Cd in plant tissues decreased with increased stem diameter, increased age of stems and stands as well as increased biomass growth. Thus, concentrations of Cd in Salix material, in clones from some of the first generation of energy forest plants (S. viminalis, S. dasyclados and S. schwerinii). were fairly high in young stands. In general, 1-year-old shoots contained 5-6 ppm Cd of dry solids in stems and 10-15 ppm in leaves. In older shoots, up to three years, and older stands, up to six years, the Cd contents decreased to 1-2 ppm Cd and 2-4 ppm Cd of dry solids for stems and leaves, respectively. Abundant supplies of water and nutrients seem to stimulate the Cd uptake in Salix in terms of higher concentrations in plant tissues as well as higher plant growth levels compared with conventional energy forestry. Stands with older stems, not irrigated and not fully fertilized resulted in uptake rates in the range of 10-30 g Cd per hectare and year. 14 refs, 9 figs, 1 tab

  17. Influence of flooding and metal immobilising soil amendments on availability of metals for willows and earthworms in calcareous dredged sediment-derived soils

    Energy Technology Data Exchange (ETDEWEB)

    Vandecasteele, Bart, E-mail: bart.vandecasteele@ilvo.vlaanderen.b [Institute for Agricultural and Fisheries Research (ILVO), Scientific Institute of the Flemish Government, Burg. Van Gansberghelaan 109, B-9820 Merelbeke (Belgium); Du Laing, Gijs [Ghent University, Department of Applied Analytical and Physical Chemistry, Coupure 653, B-9000 Ghent (Belgium); Lettens, Suzanna [Research Institute for Nature and Forest (INBO), Scientific Institute of the Flemish Government, Gaverstraat 4, B-9500 Geraardsbergen (Belgium); Jordaens, Kurt [Department of Biology, Evolutionary Biology Group, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp (Belgium); Tack, Filip M.G. [Ghent University, Department of Applied Analytical and Physical Chemistry, Coupure 653, B-9000 Ghent (Belgium)

    2010-06-15

    Soil amendments previously shown to be effective in reducing metal bioavailability and/or mobility in calcareous metal-polluted soils were tested on a calcareous dredged sediment-derived soil with 26 mg Cd/kg dry soil, 2200 mg Cr/kg dry soil, 220 mg Pb/kg dry soil, and 3000 mg Zn/kg dry soil. The amendments were 5% modified aluminosilicate (AS), 10% w/w lignin, 1% w/w diammonium phosphate (DAP, (NH{sub 4}){sub 2}HPO{sub 4}), 1% w/w MnO, and 5% w/w CaSO{sub 4}. In an additional treatment, the contaminated soil was submerged. Endpoints were metal uptake in Salix cinerea and Lumbricus terrestris, and effect on oxidation-reduction potential (ORP) in submerged soils. Results illustrated that the selected soil amendments were not effective in reducing ecological risk to vegetation or soil inhabiting invertebrates, as metal uptake in willows and earthworms did not significantly decrease following their application. Flooding the polluted soil resulted in metal uptake in S. cinerea comparable with concentrations for an uncontaminated soil. - Some soil amendments resulted in higher metal uptake by earthworms and willows than when the polluted soil was not amended but submersion of the polluted soil resulted in reduced Cd and Zn uptake in Salix cinerea.

  18. Yield Mapping in Salix; Skoerdekartering av salix

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, Christoffer; Gilbertsson, Mikael; Rogstrand, Gustav; Thylen, Lars

    2004-09-01

    The most common species for energy forest production is willow. Willow is able to produce a large amount of biomass in a short period of time. Growing willow has a potential to render a good financial result for the farmer if cultivated on fields with the right conditions and plenty of water. Under the right conditions growing willow can give the farmer a net income of 3,000 SEK (about 430 USD) per hectare and year, which is something that common cereal crops cannot compete with. However, this is not the common case since willow is often grown as a substitute crop on fields where cereal crop yield is low. The aim of this study was to reveal if it is possible to measure yield variability in willow, and if it is possible to describe the reasons for yield variation both within the field but also between different fields. Yield mapping has been used in conventional farming for about a decade. The principles for yield mapping are to continuously measure the yield while registering location by the use of GPS when harvesting the field. The collected data is then used to search for spatial variations within the field, and to try to understand the reasons for this variation. Since there is currently no commercial equipment for yield mapping in willow, a yield mapping system had to be developed within this project. The new system was installed on a Claas Jaguar harvester. The principle for yield mapping on the Claas Jaguar harvester is to measure the distance between the feeding rollers. This distance is correlated to the flow through the harvester. The speed and position of the machine was registered using GPS. Knowing the working width of the harvester this information was used to calculate the yield. All collected data was stored on a PDA computer. Soil samples were also collected from the yield mapped fields. This was to be able to test yield against both physical and chemical soil parameters. The result shows that it is possible to measure spatial variations of yield in

  19. The neutral species of the weak base trimethoprim is more toxic to willow trees (Salix viminalis) than the cation

    DEFF Research Database (Denmark)

    Mikes, O.; Trapp, Stefan

    2011-01-01

    The acute toxicity of the veterinary antibiotic trimethoprim (TMP) to willow trees was tested at three different pH levels in hydroponic solutions with TMP concentrations of 1, 10, 100 or 1000 mg/L. The pH variation was achieved by using ammonium (pH 4.3, low) or nitrate (pH 6.4, medium) as nitro......The acute toxicity of the veterinary antibiotic trimethoprim (TMP) to willow trees was tested at three different pH levels in hydroponic solutions with TMP concentrations of 1, 10, 100 or 1000 mg/L. The pH variation was achieved by using ammonium (pH 4.3, low) or nitrate (pH 6.4, medium...

  20. Can liming change root anatomy, biomass allocation and trace element distribution among plant parts of Salix × smithiana in trace element-polluted soils?

    Science.gov (United States)

    Vondráčková, Stanislava; Tlustoš, Pavel; Száková, Jiřina

    2017-08-01

    Willows (Salix spp.) are considered to be effective for the phytoremediation of trace elements from contaminated soils, but their efficiency is limited in heavily polluted soils because of poor growth. Liming can be a desirable measure to decrease the plant availability of elements, resulting in improved plant development. Notably, large root area and maximum soil penetration are basic parameters that improve the efficiency of phytoremediation. The impact of soil chemical properties on willow root anatomy and the distribution of trace elements below-ground have rarely been studied. The effect of liming on root parameters, biomass allocation and trace element distribution in non-harvestable (coarse roots, fine roots, stumps) and harvestable plant parts (twigs and leaves) of Salix × smithiana was assessed at the end of a 4-year pot experiment with two trace element-polluted soils that differed in terms of soil pH. Stump biomass predominated in weakly acidic soil. In neutral soil, the majority of biomass was located in fine roots and stumps; the difference from other plant parts was minor. Trace elements were the most concentrated in fine roots. Translocation to above-ground biomass increased as follows: Pb roots roots). Lime application decreased the concentrations of mobile Cd and Zn and related levels in plants, improved biomass production and root parameters and increased the removal of all trace elements in weakly acidic soil. None or minimum differences in the monitored parameters were recorded for dolomite treatments in both soils. The dose and source of liming had crucial effects on root anatomy. Growing willows in limed trace element-polluted soils is a suitable measure for combination of two remediation strategies, i.e. phytoextraction of Cd and Zn and assisted phytostabilization of As and Pb.

  1. Phytoextraction of initial cutting of Salix matsudana for Cd and Cu.

    Science.gov (United States)

    Wang, Wen-Wen; Cheng, Liu Ke; Hao, Jie Wei; Guan, Xin; Tian, Xing-Jun

    2016-06-27

    Salix species are widely used as vegetation filters because of their flourishing root system and fast growth rate. However, studies have yet to determine whether the root system functions in vegetable filters with mixed heavy metal (HM) pollution or whether initial cutting participates in the phytoextraction of HMs. This study aims to determine the function of the root system and initial cutting as vegetation filters in the absorption and accumulation of Cd and Cu. Thick (>1 cm in diameter) and fine (phytoextraction capacity of plants. The initial cuttings could also absorb and accumulate HMs in the early growth stages of willow without roots. Cu inhibited the plant absorption and accumulation of Cd and promoted Cd transport to shoots. Cd inhibited the Cu absorption of the root system. Our study provided essential data regarding woody species as vegetation filters of HM pollution.

  2. Energy balance and evaporation of a short-rotation willow forest. Variation with season and stand development

    Energy Technology Data Exchange (ETDEWEB)

    Iritz, Z.

    1996-10-01

    Energy balance and evaporation of a short-rotation willow (Salix viminalis L.) forest was studied in relation to season and stand development. The developmental stage of the forest stand considerably influenced how the energy, received as net radiation, was partitioned between the connective fluxes and the storage components. The main part of the available energy was utilised for evaporation during most of the season. Only at the beginning of the season did the willow forest supply heat to the atmosphere. Later in the season, energy was taken from air and utilised for evaporation, which resulted in negative sensible heat fluxes. Soil heat storage was also a significant term in the energy balance and also strongly depended on canopy development. Changes in energy partitioning relative to leaf area indices indicated the existence of a threshold value for leaf area index of the developing canopy. The analysis suggested that the canopy of the willow forest could be considered as closed at a leaf area index of 2. It was further found that evaporation from well-irrigated willow forest occurred also during night-time, particularly in windy and dry weather conditions. The sources of nocturnal evaporation were both the canopy, i.e. indicating non-closed stomata, and the soil surface. Partitioning of the total evaporation into components was investigated using a physically-based model with a two-layer aboveground representation and a two-layer soil module. The model estimates evaporation with respect to developmental stage of the willow stand and also takes into account the interaction between the fluxes from the canopy and the soil surface. Good performance of the model indicated that, after further testing in drier conditions, it could be used as a tool for analysing the prerequisites for energy-forest establishment, and practical management of energy forest stands. 37 refs, 9 figs

  3. Quality Testing of Short Rotation Coppice Willow Cuttings

    Directory of Open Access Journals (Sweden)

    Katrin Heinsoo

    2018-06-01

    Full Text Available The production and feasibility of Short Rotation Coppice depend on cutting early performance. The shoot and root biomass production of Salix cuttings in hydroponic conditions was studied. The amount of sprouted biomass after four weeks of growth depended on cutting the diameter, but the original position of the cutting along the rod or number of visible buds was not in correlation with biomass produced. Application of mineral fertilizer or soil originating from the willow plantation did not increase the total production. On the contrary, the addition of soil tended to decrease biomass production and we assumed this was a result of a shortage of light. Under the influence of fertilization, plants allocated greater biomass to roots. Comparison of different clones revealed that those with S. dasyclados genes tended to allocate less biomass to roots and the poorest-performing clone in our experiment, also had the lowest wood production in the plantation. The number of visible buds on the cutting was also clone-specific.

  4. Positive impact of bio-stimulators on growth and physiological activity of willow in climate change conditions

    Science.gov (United States)

    Piotrowski, Krzysztof; Romanowska-Duda, Zdzisława

    2018-04-01

    The aim of this research was to evaluate the physiological activity and growth of willow (Salix viminalis L.) plants cultivated under the conditions of adverse temperature and soil moisture content, and to assess the effect of the foliar application of Biojodis (1.0%) and Asahi SL (0.03%) bio-stimulators, or a mixture of Microcistis aeruginosa MKR 0105 and Anabaena PCC 7120 cyanobacteria under such changing growth conditions. The obtained results showed different reactions to the applied constant or periodically changed temperature and soil moisture content. The plants which grew at periodically changed adverse temperature (from -5 to 40oC) or in scantily (20% m.c.) or excessively (60% m.c.) watered soils, grew slowly, in comparison with those growing at 20oC and in optimally moistened soil (30% m.c.). Foliar application of Biojodis and Asahi SL cyanobacteria increased the growth of willow at optimal and adverse temperature or in scantily and excessively moistened soil. The changes in plant growth were associated with the changes in electrolyte leakage, activity of acid or alkaline phosphatases, RNase, index of chlorophyll content in leaves and gas exchange. The above indicates that the foliar application of the studied cyanobacteria and bio-stimulators partly alleviates the harmful impact of adverse temperature and water stress on growth and physiological activity of willow plants

  5. The discoloration illusion.

    Science.gov (United States)

    Pinna, Baingio

    2006-01-01

    The discoloration illusion, a new visual phenomenon, is described. This phenomenon originates from the juxtaposition of eight chromatic parallel contours on a white background, creating a luminance gradient and enclosing a light red region. Under these conditions, the inner region appears white: the light red discolors and appears white with both surface color and luminous qualities. In two experiments, the discoloration illusion was (i) compared with the coloration effect of the watercolor illusion, obtained when the number of adjacent contours was reduced to at least two, and (ii) tested under several conditions useful for understanding the roles of the luminance gradient profile. The results suggest that discoloration is not a lightness illusion and does not depend on simultaneous contrast or on achromatic mechanisms, but more likely on chromatic mechanisms that, through the luminance chromatic gradient, provide cues about the interactions of light and surface and model the volume by depicting lights and shades. The discoloration illusion suggests a possible neural scenario where multiple juxtaposed contours may stimulate neurons, selective for different asymmetric luminance profiles and signaling not only the unilateral belongingness of the boundaries and the coloration effect but also the volumetric and the illumination effects.

  6. The effect of hydrological regime on the metal bioavailability for the wetland plant species Salix cinerea

    International Nuclear Information System (INIS)

    Vandecasteele, Bart; Quataert, Paul; Tack, Filip M.G.

    2005-01-01

    The hydrological conditions on a site constitute one of the many factors that may affect the availability of potentially toxic trace metals for uptake by plants. Bioavailability of Cd, Mn and Zn in a contaminated dredged sediment-derived soil under different hydrological regimes was determined by measuring metal uptake by the wetland plant species Salix cinerea, both in field circumstances and in a greenhouse experiment. Longer submersion periods in the field caused lower Cd concentrations in leaves and bark. The wetland hydrological regime in the greenhouse experiment resulted in normal Cd and Zn concentrations in the leaves, while the upland hydrological regime resulted in elevated Cd and Zn concentrations in the leaves. Field observations and the greenhouse experiment suggest that a hydrological regime that creates or sustains a wetland is a potential management option that reduces metal bioavailability to willows. This would constitute a safe management option of metal-polluted, willow-dominated wetlands provided that wetland conditions can be maintained throughout the full growing season. - A hydrological regime aiming at wetland creation is a potential management option that favors reducing Cd plant availability in polluted freshwater wetlands

  7. Translocation of Cd and Mn from Bark to Leaves in Willows on Contaminated Sediments: Delayed Budburst Is Related to High Mn Concentrations

    Directory of Open Access Journals (Sweden)

    Bart Vandecasteele

    2015-04-01

    Full Text Available Changes in the hydrology of sediments in tidal marshes or landfills may affect the uptake of metals in the vegetation. Leaf and stem samples of Salix cinerea (grey sallow were collected during four consecutive growing seasons at six contaminated plots on a polluted dredged sediment landfill and one plot on an uncontaminated reference site. The first three contaminated plots were already emerged in the first half of the first growing season, while the other three were submerged in the first year, but became increasingly dry over the study period. Foliar and stem cutting concentrations for Cd, Zn and Mn increased on the latter three plots over the four years. Willow bark contained high concentrations of Cd, Zn and Mn. In two consecutive greenhouse experiments with willow cuttings from different origins (uncontaminated and contaminated sites and grown under different soil conditions (uncontaminated and contaminated, we observed an important translocation of Mn from bark to shoots. In a third experiment with willow cuttings collected on soils with a range of heavy metal concentrations and, thus, with a broad range of Cd (4–67 mg/kg dry matter, Zn (247–660 mg/kg dry matter and Mn (38–524 mg/kg dry matter concentrations in the bark, high Mn concentrations in the bark were found to affect the budburst of willow cuttings, while no association of delayed budburst with Cd and Zn concentrations in the bark was found. We conclude that wood and, especially, bark are not a sink for metals in living willows. The high Mn concentrations in the bark directly or indirectly caused delayed or restricted budburst of the willow cuttings.

  8. Vegetative reproduction capacities of floodplain willows--cutting response to competition and biomass loss.

    Science.gov (United States)

    Radtke, A; Mosner, E; Leyer, I

    2012-03-01

    While several studies on regeneration in Salicaceae have focused on seedling recruitment, little is known about factors controlling their vegetative reproduction. In two greenhouse experiments, we studied the response of floodplain willows (Salix fragilis, S. viminalis, S. triandra) to competition with Poa trivialis, and to shoot and root removal when planted as vegetative cuttings. In the first experiment, growth performance variables were analysed in relation to full competition, shoot competition, root competition and control, taking into account two different water levels. After 9 weeks, shoots were removed and the resprouting capacity of the bare cuttings was recorded. In the second experiment, the cutting performance of the three floodplain and an additional two fen willow species (S. cinerea, S. aurita) was compared when grown in three different soil compositions and with two different water levels. After 9 weeks, shoot and root biomass was removed and the bare cuttings were replanted to test their ability to resprout. Cutting performance and secondary resprouting were negatively affected by full and shoot competition while root competition had no or weak effects. The floodplain species performed better than the fen species in all soil types and water levels. Secondary resprouting capacity was also higher in the floodplain species, which showed an additional strong positive response to the previous waterlogging treatment. The results contribute to understanding of the vegetative regeneration ecology of floodplain willows, and suggest that the use of vegetative plantings in restoration plantings could be an effective strategy for recovering floodplain forests. © 2011 German Botanical Society and The Royal Botanical Society of the Netherlands.

  9. Cadmium in Salix. A study to show the capacity of Salix to remove cadmium from farmland

    International Nuclear Information System (INIS)

    Oestman, G.

    1994-01-01

    The aim of this report has been to show the ability of Salix to take up cadmium and how the uptake varies between different types of soil. The information that the results are based on has been obtained from analyses of soil and Salix. The samples were taken at five sites in the district around Lake Maelaren. Two or three stands were taken at each place. The factors studied were the pH, the organic matter content, and the concentration of cadmium in the soil. Salix has a good ability, relative to other crops, to remove cadmium from arable land. The cadmium uptake is 35 times higher with Salix than with straw or energy grass. Salix uptake of cadmium varies between 3 and 14% of the cadmium content in the soil that is accessible to plants. The present annual increase of cadmium in arable land is 1 g/ha, whereas the removal in a Salix plantation is 21 g Cd/ha, yr at an annual growth of 10 tonnes DM. If the Cd uptake is the same each year, then a total of 420 g Cd/ha is removed when Salix is grown over a 20-year period. This is a very large part of the topsoil's total cadmium content, which is 550 g/ha on average in Sweden. The investigation reveals no clear relationship between the Cd concentration in Salix and the concentration of Cd in the soil, the organic matter content or the pH. 22 refs, 4 figs, 2 tabs

  10. Investigation of Solid Energy Potential of Wood and Bark Obtained from Four Clones of a 2-Year Old Goat Willow

    International Nuclear Information System (INIS)

    Han, Sim-Hee; Shin, Soo-Jeong

    2014-01-01

    To investigate the solid raw material characteristics of willow (Salix caprea) bark and woody core, this study analyzed overall chemical composition, monosaccharide composition, ash content, and main ash composition of both tree components. Significant differences were observed between the two in terms of chemical composition, carbohydrate composition, ash content, and major inorganics. The ash content in bark was 3.8–4.7%, compared with 0.6–1.1% in the woody core. Polysaccharide content in the woody core was 62.8–70.6% but was as low as 44.1–47.6% in the bark. The main hemicelluloses consisting of monosaccharides were xylose in the case of the woody core, and xylose, galactose, and arabinose in the case of bark. Woody core biomass of willow provides superior solid fuel raw material, as compared with bark biomass, with higher heating values, less ash content, and less slagging-causing material.

  11. Investigation of Solid Energy Potential of Wood and Bark Obtained from Four Clones of a 2-Year Old Goat Willow

    Energy Technology Data Exchange (ETDEWEB)

    Han, Sim-Hee [Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon (Korea, Republic of); Shin, Soo-Jeong, E-mail: soojshin@cbnu.ac.kr [Department of Wood and Paper Science, Chungbuk National University, Cheongju (Korea, Republic of)

    2014-01-31

    To investigate the solid raw material characteristics of willow (Salix caprea) bark and woody core, this study analyzed overall chemical composition, monosaccharide composition, ash content, and main ash composition of both tree components. Significant differences were observed between the two in terms of chemical composition, carbohydrate composition, ash content, and major inorganics. The ash content in bark was 3.8–4.7%, compared with 0.6–1.1% in the woody core. Polysaccharide content in the woody core was 62.8–70.6% but was as low as 44.1–47.6% in the bark. The main hemicelluloses consisting of monosaccharides were xylose in the case of the woody core, and xylose, galactose, and arabinose in the case of bark. Woody core biomass of willow provides superior solid fuel raw material, as compared with bark biomass, with higher heating values, less ash content, and less slagging-causing material.

  12. 7 CFR 51.638 - Discoloration.

    Science.gov (United States)

    2010-01-01

    ...) Definitions § 51.638 Discoloration. Discoloration means russeting of light shade of golden brown caused by... area, provided no discoloration caused by speck type melanose or other means may detract from the... Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing...

  13. Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1β-Induced NF-κB Activation in Canine Articular Chondrocytes

    Science.gov (United States)

    Shakibaei, Mehdi; Allaway, David; Nebrich, Simone; Mobasheri, Ali

    2012-01-01

    The aim of this study was to characterize the anti-inflammatory mode of action of botanical extracts from rosehip (Rosa canina), willow bark (Salix alba), and nettle leaf (Urtica dioica) in an in vitro model of primary canine articular chondrocytes. Methods. The biological effects of the botanical extracts were studied in chondrocytes treated with IL-1β for up to 72 h. Expression of collagen type II, cartilage-specific proteoglycan (CSPG), β1-integrin, SOX-9, COX-2, and MMP-9 and MMP-13 was examined by western blotting. Results. The botanical extracts suppressed IL-1β-induced NF-κB activation by inhibition of IκBα phosphorylation, IκBα degradation, p65 phosphorylation, and p65 nuclear translocation. These events correlated with downregulation of NF-κB targets including COX-2 and MMPs. The extracts also reversed the IL-1β-induced downregulation of collagen type II, CSPG, β1-integrin, and cartilage-specific transcription factor SOX-9 protein expression. In high-density cultures botanical extracts stimulated new cartilage formation even in the presence of IL-1β. Conclusions. Botanical extracts exerted anti-inflammatory and anabolic effects on chondrocytes. The observed reduction of IL-1β-induced NF-κB activation suggests that further studies are warranted to demonstrate the effectiveness of plant extracts in the treatment of OA and other conditions in which NF-κB plays pathophysiological roles. PMID:22474508

  14. Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1β-Induced NF-κB Activation in Canine Articular Chondrocytes.

    Science.gov (United States)

    Shakibaei, Mehdi; Allaway, David; Nebrich, Simone; Mobasheri, Ali

    2012-01-01

    The aim of this study was to characterize the anti-inflammatory mode of action of botanical extracts from rosehip (Rosa canina), willow bark (Salix alba), and nettle leaf (Urtica dioica) in an in vitro model of primary canine articular chondrocytes. Methods. The biological effects of the botanical extracts were studied in chondrocytes treated with IL-1β for up to 72 h. Expression of collagen type II, cartilage-specific proteoglycan (CSPG), β1-integrin, SOX-9, COX-2, and MMP-9 and MMP-13 was examined by western blotting. Results. The botanical extracts suppressed IL-1β-induced NF-κB activation by inhibition of IκBα phosphorylation, IκBα degradation, p65 phosphorylation, and p65 nuclear translocation. These events correlated with downregulation of NF-κB targets including COX-2 and MMPs. The extracts also reversed the IL-1β-induced downregulation of collagen type II, CSPG, β1-integrin, and cartilage-specific transcription factor SOX-9 protein expression. In high-density cultures botanical extracts stimulated new cartilage formation even in the presence of IL-1β. Conclusions. Botanical extracts exerted anti-inflammatory and anabolic effects on chondrocytes. The observed reduction of IL-1β-induced NF-κB activation suggests that further studies are warranted to demonstrate the effectiveness of plant extracts in the treatment of OA and other conditions in which NF-κB plays pathophysiological roles.

  15. Botanical Extracts from Rosehip (Rosa canina, Willow Bark (Salix alba, and Nettle Leaf (Urtica dioica Suppress IL-1β-Induced NF-κB Activation in Canine Articular Chondrocytes

    Directory of Open Access Journals (Sweden)

    Mehdi Shakibaei

    2012-01-01

    Full Text Available The aim of this study was to characterize the anti-inflammatory mode of action of botanical extracts from rosehip (Rosa canina, willow bark (Salix alba, and nettle leaf (Urtica dioica in an in vitro model of primary canine articular chondrocytes. Methods. The biological effects of the botanical extracts were studied in chondrocytes treated with IL-1β for up to 72 h. Expression of collagen type II, cartilage-specific proteoglycan (CSPG, β1-integrin, SOX-9, COX-2, and MMP-9 and MMP-13 was examined by western blotting. Results. The botanical extracts suppressed IL-1β-induced NF-κB activation by inhibition of IκBα phosphorylation, IκBα degradation, p65 phosphorylation, and p65 nuclear translocation. These events correlated with downregulation of NF-κB targets including COX-2 and MMPs. The extracts also reversed the IL-1β-induced downregulation of collagen type II, CSPG, β1-integrin, and cartilage-specific transcription factor SOX-9 protein expression. In high-density cultures botanical extracts stimulated new cartilage formation even in the presence of IL-1β. Conclusions. Botanical extracts exerted anti-inflammatory and anabolic effects on chondrocytes. The observed reduction of IL-1β-induced NF-κB activation suggests that further studies are warranted to demonstrate the effectiveness of plant extracts in the treatment of OA and other conditions in which NF-κB plays pathophysiological roles.

  16. 7 CFR 51.700 - Discoloration.

    Science.gov (United States)

    2010-01-01

    ... § 51.700 Discoloration. Discoloration means russeting of light shade of golden brown caused by rust..., provided no discoloration caused by melanose or other means may affect the appearance of the fruit to a... Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing...

  17. Fiber length and pulping characteristics of switchgrass, alfalfa stems, hybrid poplar and willow biomasses.

    Science.gov (United States)

    Ai, Jun; Tschirner, Ulrike

    2010-01-01

    Switchgrass (Panicum virgatum), alfalfa stems (Medicago sativa), second year growth hybrid poplar (Populus) and willow (Salix spp.) were examined to determine fiber characteristics, pulping behavior and paper properties. Alfalfa stems and switchgrass both showed length weighted average fiber length (LWW) of 0.78 mm, a very narrow fiber length distribution and high fines content. Willow and hybrid poplar have lower fines content but a very low average fiber length (0.42 and 0.48 mm LWW). In addition, the four biomass species showed distinctly different chemical compositions. Switchgrass was defibered successfully using Soda and Soda Anthraquinone (AQ) pulping and demonstrated good paper properties. Both fast-growing wood species pulped well using the Kraft process, and showed acceptable tensile strength, but low tear strength. Alfalfa stems reacted very poorly to Soda and Soda AQ pulping but responded well to Kraft and Kraft AQ. Pulps with tensile and tear strength considerably higher than those found for commercial aspen pulps were observed for alfalfa. All four biomass species examined demonstrated low pulp yield. The highest yields were obtained with poplar and switchgrass (around 43%). Considering the short fibers and low yields, all four biomass types will likely only be used in paper manufacturing if they offer considerable economic advantage over traditional pulp wood.

  18. VOC emissions and carbon balance of two bioenergy plantations in response to nitrogen fertilization: A comparison of Miscanthus and Salix.

    Science.gov (United States)

    Hu, Bin; Jarosch, Ann-Mareike; Gauder, Martin; Graeff-Hönninger, Simone; Schnitzler, Jörg-Peter; Grote, Rüdiger; Rennenberg, Heinz; Kreuzwieser, Jürgen

    2018-06-01

    Energy crops are an important renewable source for energy production in future. To ensure high yields of crops, N fertilization is a common practice. However, knowledge on environmental impacts of bioenergy plantations, particularly in systems involving trees, and the effects of N fertilization is scarce. We studied the emission of volatile organic compounds (VOC), which negatively affect the environment by contributing to tropospheric ozone and aerosols formation, from Miscanthus and willow plantations. Particularly, we aimed at quantifying the effect of N fertilization on VOC emission. For this purpose, we determined plant traits, photosynthetic gas exchange and VOC emission rates of the two systems as affected by N fertilization (0 and 80 kg ha -1 yr -1 ). Additionally, we used a modelling approach to simulate (i) the annual VOC emission rates as well as (ii) the OH . reactivity resulting from individual VOC emitted. Total VOC emissions from Salix was 1.5- and 2.5-fold higher compared to Miscanthus in non-fertilized and fertilized plantations, respectively. Isoprene was the dominating VOC in Salix (80-130 μg g -1 DW h -1 ), whereas it was negligible in Miscanthus. We identified twenty-eight VOC compounds, which were released by Miscanthus with the green leaf volatile hexanal as well as dimethyl benzene, dihydrofuranone, phenol, and decanal as the dominant volatiles. The pattern of VOC released from this species clearly differed to the pattern emitted by Salix. OH . reactivity from VOC released by Salix was ca. 8-times higher than that of Miscanthus. N fertilization enhanced stand level VOC emissions, mainly by promoting the leaf area index and only marginally by enhancing the basal emission capacity of leaves. Considering the higher productivity of fertilized Miscanthus compared to Salix together with the considerably lower OH . reactivity per weight unit of biomass produced, qualified the C 4 -perennial grass Miscanthus as a superior source of future

  19. Discoloration Potential of Endodontic Sealers: A Brief Review

    Science.gov (United States)

    Tour Savadkouhi, Sohrab; Fazlyab, Mahta

    2016-01-01

    Tooth discoloration induced by endodontic sealers, is a common finding that impairs aesthetic outcome of endodontic treatment. The aim of the present mini literature review, was to summarize the existing data on discoloration potential of different endodontic sealers. The research covered the article published in PubMed and Google Scholar from 2000 to 2015. The searched keywords included ‘tooth discoloration AND endodontic’, ‘tooth discoloration AND sealer, ‘tooth discoloration AND zinc-oxide eugenol sealer’, ‘tooth discoloration AND Calcium Hydroxide Sealer’, ‘tooth discoloration AND Glass Ionomer Sealer’, ‘tooth discoloration AND epoxy-resin Sealer’, ‘tooth discoloration AND Silicon Based Sealer’, ‘tooth discoloration AND Bioceramic Sealer’ and ‘Spectrophotometry’. Conclusion: A total number of 44 articles were gained which reduced to 11 after excluding the repetitive items. The available evidence for discoloration potential of endodontic sealers currently available on the market is scarce. However, it can be concluded that all endodontic sealers can potentially stain the tooth structure to different degrees. PMID:27790251

  20. Enzymatic Saccharification of Shrub Willow Genotypes with Differing Biomass Composition for Biofuel Production

    Directory of Open Access Journals (Sweden)

    Michelle J. Serapiglia

    2013-03-01

    Full Text Available In the conversion of woody biomass feedstocks into liquid fuel ethanol, the pretreatment process is the most critical and costly step. Variations in biomass composition based on genetic differences or environmental effects have a significant impact on the degree of accessibility accomplished by pretreatment and subsequent sugar release by enzymatic hydrolysis. To evaluate this, biomass from 10 genetically diverse, genotypes of shrub willow (Salix spp. was pretreated with a hot-water process at two levels of severity, hydrolyzed using a combination of two commercial enzyme cocktails, and the release of hexose and pentose monomers was quantified by HPLC. Among the genotypes selected for analysis, cellulose content ranged from 39 to 45% (w/w and lignin content ranged from 20 to 23% (w/w at harvest. Differences in the effectiveness of the pretreatment process were observed among the various willow genotypes. Correlations were identified between total sugar release and % cellulose and % lignin content. There was a significant effect of pretreatment severity on polysaccharide accessibility, but the response to pretreatments was different among the genotypes. At the high severity pretreatment ‘SV1’ was the least recalcitrant with sugar release representing as much as 60% of total biomass. These results suggest that structural, as well as chemical characteristics of the biomass may influence pretreatment and hydrolytic efficiency.

  1. Cultural Resources Investigation of a Proposed Flood Control Project along the Sheyenne River, at West Fargo, Cass County, North Dakota. Phase I.

    Science.gov (United States)

    1988-01-15

    cottonwood (Po2ulus salrgenti), sumac (Ehus sp.), peach and sandbar willows (Salix amvdaloides; . terio), and slippery elm (Ulmus rubra). 3.4.2 Bluestem...forest of elm , oak, ash, hackberry, cottonwood, and aspen along the major streams. Archaeological evidence indicates that prehistoric people along the...black willow (Salix nifra), and American elm (Ulmus amicnan). Other components include: boxelder (Acer neaundo), red maple (A. Subrum), silver maple (A

  2. Salix transect of Europe: structured genetic variation and isolation-by-distance in the nettle psyllid, Trioza urticae (Psylloidea, Hemiptera), from Greece to Arctic Norway

    Science.gov (United States)

    Wonglersak, Rungtip; Cronk, Quentin; Percy, Diana

    2017-01-01

    Abstract Background The common nettle (Urtica dioica L.) is co-associated with willows (Salix spp.) in riparian habitats across Europe. We sampled the widespread nettle psyllid, Trioza urticae (Linné, 1758), from Urtica in willow habitats on a megatransect of Europe from the Aegean to the Arctic Ocean. The aim of this study was to use an unusually widespread insect to assess the influence of geographic distances and natural geographic barriers on patterns of genetic variation and haplotype distribution. New information Phylogeographic analysis using DNA sequences of two mtDNA regions, COI and cytB, shows that T. urticae specimens are organized into four regional groups (southern, central, northern and arctic). These groups are supported by both phylogenetic analysis (four geographically-based clades) and network analysis (four major haplotype groups). The boundary between southern and central groups corresponds to the Carpathian Mountains and the boundary between the central and northern groups corresponds to the Gulf of Finland. Overall these groups form a latitudinal cline in genetic diversity, which decreases with increasing latitude. PMID:28325977

  3. Impact of anthropogenic forest contamination on radioresistance of woody plant seed

    International Nuclear Information System (INIS)

    Yushkov, P.I.

    2004-01-01

    Radioresistance of seeds of bay willow (Salix pentandra L.) and great sallow willow (Salix caprea L.) from forests chronically affected and non-affected by acidic (SO 2 , NO x , HF, etc.) industries has been studied and compared. Bay willow seeds of 6 harvests showed no difference in radioresistance. However, seeds of both species manifested strong synchronous variability in resistance to preplant exposure. Also, no influence was observed of mother plant gas content on great sallow willow seeds of different harvests. Data obtained confirm similar results of previous studies conducted by the author (1987) to identify the impact of plant gas content on radioresistance of seeds and seedlings of pine (Pinus sylvestris L.) and birch (Betula verrucosa Erh.). (author)

  4. Vegetation Evaluation and Recommendations: Dredge Material Placement Areas and Adjacent Lands, Kaskaskia River Navigation Project, New Athens to Fayetteville.

    Science.gov (United States)

    1981-06-03

    cottonwood), Salix interior (sandbar willow), Salix nigra (black willow), and Ulmus rubra * ( slippery elm ) . Somewhat drier sites with a predominance of fine...Dutch Elm Disease. *Ulmus rubra Muhl. SLIPPERY ELM . Common tree in floodplain woods; also occasional in DMP. *Valerianella radiata (L.) Dufr. CORN... elm ), Carya tomentosa (mocker- nut hickory), and Quercus bicolor (swamp white oak). Shrubs and woody vines play a major role in the ecology of these

  5. Treatment of a Textile Effluent by Electrochemical Oxidation and Coupled System Electooxidation–Salix babylonica

    Directory of Open Access Journals (Sweden)

    Alejandra Sánchez-Sánchez

    2018-01-01

    Full Text Available The removal of pollutants from textile wastewater via electrochemical oxidation and a coupled system electrooxidation—Salix babylonica, using boron-doped diamond electrodes was evaluated. Under optimal conditions of pH 5.23 and 3.5 mA·cm−2 of current density, the electrochemical method yields an effective reduction of chemical oxygen demand by 41.95%, biochemical oxygen demand by 83.33%, color by 60.83%, and turbidity by 26.53% at 300 minutes of treatment. The raw and treated wastewater was characterized by infrared spectroscopy to confirm the degradation of pollutants. The wastewater was oxidized at 15-minute intervals for one hour and was placed in contact with willow plants for 15 days. The coupled system yielded a reduction of the chemical oxygen demand by 14%, color by 85%, and turbidity by 93%. The best efficiency for the coupled system was achieved at 60 minutes, at which time the plants achieved more biomass and photosynthetic pigments.

  6. Pollen viability of Salix myrtilloides L. – an endangered species in Poland

    Directory of Open Access Journals (Sweden)

    Magdalena Pogorzelec

    2016-12-01

    Full Text Available Salix myrtilloides L. (swamp willow is the most endangered species among the boreal Salix species in Poland. The number and size of its population have been decreasing constantly since the 1990s. The main aim of the study was to determine the viability of collected S. myrtilloides pollen and optimal conditions for its in vitro germination. The pollen of S. myrtilloides was collected from 25 male individuals from a population growing in the mid-forest peat bog Dekowina (Sobibór Landscape Park in May 2014. Two methods were applied to estimate the viability of fresh and stored pollen: staining pollen with 2% acetocarmine solution and in vitro germinability. Various temperature (11°C, 23°C and light conditions as well as different concentrations of glucose (1%, 2.5%, 5%, or 7.5% were tested for the optimization of in vitro germination. We documented relatively high S. myrtilloides pollen viability. Pollen tube growth was found to be largely affected by both glucose content in the medium and thermal conditions during germination. Fresh pollen germinated most effectively on the medium with 2.5% glucose (stored pollen – in 5% glucose, at 23°C and in the presence of light. We conclude that pollen viability of S. myrtilloides does not seem to be a limiting factor for reproductive success. Moreover, the pollen is not sterile even after storage for 12 months. The S. myrtilloides individuals from the Dekowina peat bog produce viable pollen grains that are able to germinate and therefore it can be used to pollinate other populations present in the Polesie Lubelskie region for gene pool enrichment.

  7. Aesthetic treatment of discoloration of nonvital teeth.

    Science.gov (United States)

    Miara, P

    1995-09-01

    Attempts to treat discoloration in nonvital teeth were first reported a century ago. This article discusses two potential causes of nonvital tooth discoloration-trauma and endodontic treatment-along with a step-by-step clinical procedure for treatment of the discoloration. In trauma, hemoglobin is released into the tissues; iron oxides, formed by oxygen and iron in hemoglobin, cause discoloration and swelling that infringes on pulp space, forcing the pulp to recede with a potential loss of tooth vitality. After endodontic treatment, either hemorrhaging, materials used, or incomplete removal and breakdown of necrotic tissue may cause staining. The learning objective of this article is to review the causes and the prevention/treatment of discoloration in nonvital teeth.

  8. Development of a method for testing the susceptibility of Salix alba to Erwinia salicis

    Energy Technology Data Exchange (ETDEWEB)

    Dingjan-Versteegh, A M; de Kam, M [Institute for Forestry and Urban Ecology, Wageningen (Netherlands)

    1990-01-01

    Salix is one of the faster growing tree species that has potential for biomass plantations in Europe. This potential, however, is limited by the occurrence of a vascular disease caused by the bacterium Erwinia salicis (Day) Chester. Selection and breeding of disease resistance is hampered by insufficient knowledge of the infection biology and ecology of the pathogen. The availability of specific antisera is of vital importance in the study of the infection biology and in monitoring bacterial populations inside the host. Therefore, one of the aims of the research was to improve the antisera. The composition of the soluble antigens of E. salicis was studied by enzyme-linked immunosorbent assay (ELISA), polyacrylamide gel electrophoresis (PAGE) and electro-immunoblotting. Antiserum prepared to whole cells of E. salicis mainly contains antibodies to bacterial surface antigens or to soluble antigens. ELISA demonstrated the presence of bacterial soluble antigens in leaves of diseased willow trees and also in leaves of willow shoots which were placed in a solution of bacterial soluble antigens. An inoculation experiment was carried out to ascertain if there is a statistically significant correlation between plant water potential and response to artificial inoculation with E. salicis. To elucidate the mechanisms involved in pathogenicity and virulence of E. salicis, attempts were made to isolate the plasmids of the bacterium, however, it appears that E. salicis does not have plasmids. In collaboration with Ch. Maceau (21), the E. salicis genome was isolated and a genomic library was constructed in E. coli. 28 refs., 10 figs., 4 tabs.

  9. Eighteen microsatellite loci in Salix arbutifolia (Salicaceae) and cross-species amplification in Salix and Populus species.

    Science.gov (United States)

    Hoshikawa, Takeshi; Kikuchi, Satoshi; Nagamitsu, Teruyoshi; Tomaru, Nobuhiro

    2009-07-01

    Salix arbutifolia is a riparian dioecious tree species that is of conservation concern in Japan because of its highly restricted distribution. Eighteen polymorphic loci of dinucleotide microsatellites were isolated and characterized. Among these, estimates of the expected heterozygosity ranged from 0.350 to 0.879. Cross-species amplification was successful at 9-13 loci among six Salix species and at three loci in one Populus species. © 2009 Blackwell Publishing Ltd.

  10. Evaluation report. Willow; Evalueringsrapport. Pil

    Energy Technology Data Exchange (ETDEWEB)

    Bang Bilgaard, H. [AgroTech A/S, Skejby, Aarhus (Denmark); Eide, T.; Gertz, F. [Videncentret for Landbrug, Skejby, Aarhus (Denmark)] [and others

    2012-10-15

    This evaluation report brings together the main findings of willows activities in the BioM project in the period 2010-2012. The report contains sections relating to growing and cultivation conditions, energy production, environmental effects, landscape aspects, harvesting techniques, economics, and organization, marketing, and business effects, and a description of willow cultivation on a large scale in the project area in western Jutland, Denmark. The report concludes with an overall assessment of willow cultivation for the production of sustainable bioenergy. (LN)

  11. Impacts of paper sludge and manure on soil and biomass production of willow

    International Nuclear Information System (INIS)

    Quaye, Amos K.; Volk, Timothy A.; Hafner, Sasha; Leopold, Donald J.; Schirmer, Charles

    2011-01-01

    Land application of organic wastes to short rotation woody crops (SRWC) can reduce the environmental impacts associated with waste disposal and enhance the productivity of biomass production systems. Understanding the potential impacts of organic amendments however, requires the examination of changes in soil characteristics and plant productivity. This study was conducted to evaluate the effect of paper sludge and dairy manure on biomass production of shrub willow (Salix dasyclados SV1) and to determine the impacts of these amendments on soil chemical properties. Treatments included urea, dairy manure and paper sludge separately and in combination, and a control. These materials were applied in the summer of 2005 to two fields of SV1 at different stages of growth: An old field with one year old shoots on a 10 year old root system and a young field which was beginning regrowth after being coppiced at the end of its first growing season. Foliar nutrient concentrations and soil chemical properties were analyzed at the end of the second growing season after treatment application to determine plant response to the fertilization regimes and to determine the effects of fertilization on soil characteristics. Fertilization did not increase biomass production in either field. However, application of the N-poor paper sludge did not reduce yield either. In general, fertilization did not influence soil or foliar chemistry, although there were some exceptions. The lack of response observed in this study is probably related to the nutrient status of the site or losses of applied nutrients. -- Highlights: → The fertilization treatments did not have any significant effect biomass production. → Application of paper sludge did not reduce willow biomass yield in both fields. → Foliar N concentration of willow crops in this study is in the range considered for optimal growth. → The limited response of foliar nutrients to fertilization indicates that the site was not limited by

  12. Distribution of P, K, Ca, Mg, Cd, Cu, Fe, Mn, Pb and Zn in wood and bark age classes of willows and poplars used for phytoextraction on soils contaminated by risk elements.

    Science.gov (United States)

    Zárubová, Pavla; Hejcman, Michal; Vondráčková, Stanislava; Mrnka, Libor; Száková, Jiřina; Tlustoš, Pavel

    2015-12-01

    Fast-growing clones of Salix and Populus have been studied for remediation of soils contaminated by risk elements (RE) using short-rotation coppice plantations. Our aim was to assess biomass yield and distributions of elements in wood and bark of highly productive willow (S1--[Salix schwerinii × Salix viminalis] × S. viminalis, S2--Salix × smithiana clone S-218) and poplar (P1--Populus maximowiczii × Populus nigra, P2--P. nigra) clones with respect to aging. The field experiment was established in April 2008 on moderately Cd-, Pb- and Zn- contaminated soil. Shoots were harvested after four seasons (February 2012) and separated into annual classes of wood and bark. All tested clones grew on contaminated soils, with highest biomass production and lowest mortality exhibited by P1 and S2. Concentrations of elements, with exception of Ca and Pb, decreased with age and were higher in bark than in wood. The Salix clones were characterised by higher removal of Cd, Mn and Zn compared to the Populus clones. Despite generally higher RE content in young shoots, partly due to lower wood/bark ratios and higher RE concentrations in bark, the overall removal of RE was higher in older wood classes due to higher biomass yield. Thus, longer rotations seem to be more effective when phytoextraction strategy is considered. Of the four selected clones, S1 exhibited the best removal of Cd and Zn and is a good candidate for phytoextraction.

  13. Structural and productive-developmental characteristics of white willow plantations of different density on humogley in Donji Srem

    Directory of Open Access Journals (Sweden)

    Andrašev Siniša

    2012-01-01

    Full Text Available The study was conducted in two plantations of white willow (Salix alba L. in Donji Srem on the humogley soil type (hydromorphic black soil, which belongs to a narrow-leaved ash forest with remote sedge (Carici remotae - Fraxinetum angustifoliae Jov. et Tom., 1979. The plantations are located in the same depression. The SP (sample plot-1 plantation is 21 years old with a 6×6 m planting spacing, and the SP-2 plantation is 27 years old with a 3×3m planting spacing. Elements of stem growth in the SP-1 plantation showed that with the white willow planting spacing of 6×6 m and a planned 25-year production cycle it is possible to obtain about 250 m3•ha-1 of timber volume, with an 80% net share of technical wood and a 20 % share of pulp wood. The plantation in SP-2 is at the age, which is well above the optimum age in terms of rational management, and the total volume at the age of 27 years is about 300 m3•ha-1, with a 53.7% net share of technical wood and a 46.3% share of pulpwood. [Projekat Ministarstva nauke Republike Srbije, br. 43007: Istraživanje klimatskih promena na životnu sredinu: praćenje uticaja, adaptacija i ublažavanje

  14. Frost related dieback in Estonian energy plantations of willows in relation to fertilisation and pathogenic bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Cambours, M.A.; Nejad, P. [Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Box 7026, 750 07 Uppsala (Sweden); Heinsoo, K. [Institute of Zoology and Botany, Estonian Agricultural University, Riia 181, 51014 Tartu (Estonia); Granhall, U. [Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, 750 07 Uppsala (Sweden)

    2006-03-15

    Two 9-year old Estonian Salix plantations suffering from dieback were studied: one situated on poor mineral soil and divided into fertilised and unfertilised plots (Saare plantation) and another growing on a well-decomposed and nitrogen-rich organic soil, without fertiliser application (Kambja plantation). Bacteria from internal tissues of visually damaged shoots from seven clones were isolated in spring and autumn. The strains were subsequently biochemically characterised and tested for ice nucleation activity and pathogenicity on Salix. Some strains were also analysed with 16S rRNA. High numbers of culturable bacteria were found, belonging mainly to Erwinia, Sphingomonas, Pseudomonas and Xanthomonas spp. Fertilised plots were significantly more colonised by bacteria than unfertilised plots and also more extensively damaged, showing a lower density of living plants after 7 years of culture. More ice nucleation active (INA) strains were found in Saare fertilised plots and at Kambja than in Saare unfertilised plots. Likewise, most pathogenic strains were isolated from Saare fertilised plots and from Kambja. For some of the willow clones studied, dieback appeared to be related to both clonal frost sensitivity and abundance of INA and pathogenic bacteria. The plantations probably suffered from the presence of high amounts of pathogens and from frost related injuries aggravated by INA bacteria. Most probably the fertilisation at Saare and the nitrogen-rich soil at Kambja created a favourable environment for bacterial development and led to high dieback levels after the first harvest. (author)

  15. Detection of Lettuce Discoloration Using Hyperspectral Reflectance Imaging.

    Science.gov (United States)

    Mo, Changyeun; Kim, Giyoung; Lim, Jongguk; Kim, Moon S; Cho, Hyunjeong; Cho, Byoung-Kwan

    2015-11-20

    Rapid visible/near-infrared (VNIR) hyperspectral imaging methods, employing both a single waveband algorithm and multi-spectral algorithms, were developed in order to discrimination between sound and discolored lettuce. Reflectance spectra for sound and discolored lettuce surfaces were extracted from hyperspectral reflectance images obtained in the 400-1000 nm wavelength range. The optimal wavebands for discriminating between discolored and sound lettuce surfaces were determined using one-way analysis of variance. Multi-spectral imaging algorithms developed using ratio and subtraction functions resulted in enhanced classification accuracy of above 99.9% for discolored and sound areas on both adaxial and abaxial lettuce surfaces. Ratio imaging (RI) and subtraction imaging (SI) algorithms at wavelengths of 552/701 nm and 557-701 nm, respectively, exhibited better classification performances compared to results obtained for all possible two-waveband combinations. These results suggest that hyperspectral reflectance imaging techniques can potentially be used to discriminate between discolored and sound fresh-cut lettuce.

  16. A profile and analysis of willow growers in Sweden

    International Nuclear Information System (INIS)

    Rosenqvist, H.; Roos, A.; Ling, E.; Hektor, B.

    1999-01-01

    Willow plantations on Swedish farmland increased considerably between 1991 and 1996. The main driving forces behind this development were (1) the 1991 introduction of an agriculture deregulation policy in Sweden which created lower grain prices and simultaneously introduced compensation for set-aside land and subsidies for willow plantations on surplus arable land, (2) higher taxes on fossil fuels, and (3) the existence of a biofuel market in Sweden based on forest fuels. This paper presents a statistical study of 1,158 willow growers in southern and central-eastern Sweden. The resulting profile of growers will help policy makers and agents in the bioenergy business design information campaigns and marketing strategies. Willow growers are described according to geographical distribution, willow parcel sizes, farm sizes, and farm types. They are compared with the population of farmers who are not growing willow. Willow growers are more often between 50-65 years of age, and they have larger farms than non-willow growers. They are less often focused on animal and milk production and more often on cereal and food crop production than are other farmers. (author)

  17. Willow Bark

    Science.gov (United States)

    ... willow bark extract, ginger root concentrate, boswellia extract, turmeric root extract, cayenne, and hyaluronic acid (Instaflex Joint ... Sensitivity to aspirin: People with ASTHMA, STOMACH ULCERS, DIABETES, GOUT, HEMOPHILIA, HYPOPROTHROMBINEMIA, or KIDNEY or LIVER DISEASE ...

  18. Growth, physiological response and phytoremoval capability of two willow clones exposed to ibuprofen under hydroponic culture.

    Science.gov (United States)

    Iori, Valentina; Zacchini, Massimo; Pietrini, Fabrizio

    2013-11-15

    Ibuprofen (IBU) is one of the most widespread pharmaceuticals in the aquatic ecosystem, despite the high removal rate that occurs in wastewater treatment plants. Phytoremediation represents a technology to improve the performance of existing wastewater treatment. This study was conducted under hydroponics to evaluate the ability of Salicaceae plants to tolerate and reduce IBU concentration in contaminated water. To this end, we combined growth, physiological and biochemical data to study the effects of different IBU concentrations on two clones of Salix alba L. Data demonstrated that clone SS5 was more tolerant and showed a higher ability to reduce IBU concentration in the solution than clone SP3. The high tolerance to IBU shown by SS5 was likely due to several mechanisms including the capacity to maintain an elevated photosynthetic activity and an efficient antioxidative defence. These results illustrate the remarkable potential of willow to phytoremediate IBU-contaminated waters in natural and constructed wetlands. Copyright © 2013 Elsevier B.V. All rights reserved.

  19. Adsorption Property and Mechanism of Oxytetracycline onto Willow Residues

    Directory of Open Access Journals (Sweden)

    Di Wang

    2017-12-01

    Full Text Available To elucidate the adsorption property and the mechanism of plant residues to reduce oxytetracycline (OTC, the adsorption of OTC onto raw willow roots (WR-R, stems (WS-R, leaves (WL-R, and adsorption onto desugared willow roots (WR-D, stems (WS-D, and leaves (WL-D were investigated. The structural characterization was analyzed by scanning electron microscopy, Fourier-transform infrared spectra, and an elemental analyzer. OTC adsorption onto the different tissues of willow residues was compared and correlated with their structures. The adsorption kinetics of OTC onto willow residues was found to follow the pseudo-first-order model. The isothermal adsorption process of OTC onto the different tissues of willow residues followed the Langmuir and Freundlich model and the process was also a spontaneous endothermic reaction, which was mainly physical adsorption. After the willow residues were desugared, the polarity decreased and the aromaticity increased, which explained why the adsorption amounts of the desugared willow residues were higher than those of the unmodified residues. These observations suggest that the raw and modified willow residues have great potential as adsorbents to remove organic pollutants.

  20. Meta-transcriptomics indicates biotic cross-tolerance in willow trees cultivated on petroleum hydrocarbon contaminated soil.

    Science.gov (United States)

    Gonzalez, Emmanuel; Brereton, Nicholas J B; Marleau, Julie; Guidi Nissim, Werther; Labrecque, Michel; Pitre, Frederic E; Joly, Simon

    2015-10-12

    High concentrations of petroleum hydrocarbon (PHC) pollution can be hazardous to human health and leave soils incapable of supporting agricultural crops. A cheap solution, which can help restore biodiversity and bring land back to productivity, is cultivation of high biomass yielding willow trees. However, the genetic mechanisms which allow these fast-growing trees to tolerate PHCs are as yet unclear. Salix purpurea 'Fish Creek' trees were pot-grown in soil from a former petroleum refinery, either lacking or enriched with C10-C50 PHCs. De novo assembled transcriptomes were compared between tree organs and impartially annotated without a priori constraint to any organism. Over 45% of differentially expressed genes originated from foreign organisms, the majority from the two-spotted spidermite, Tetranychus urticae. Over 99% of T. urticae transcripts were differentially expressed with greater abundance in non-contaminated trees. Plant transcripts involved in the polypropanoid pathway, including phenylalanine ammonia-lyase (PAL), had greater expression in contaminated trees whereas most resistance genes showed higher expression in non-contaminated trees. The impartial approach to annotation of the de novo transcriptomes, allowing for the possibility for multiple species identification, was essential for interpretation of the crop's response treatment. The meta-transcriptomic pattern of expression suggests a cross-tolerance mechanism whereby abiotic stress resistance systems provide improved biotic resistance. These findings highlight a valuable but complex biotic and abiotic stress response to real-world, multidimensional contamination which could, in part, help explain why crops such as willow can produce uniquely high biomass yields on challenging marginal land.

  1. Vessel discoloration detection in malarial retinopathy

    Science.gov (United States)

    Agurto, C.; Nemeth, S.; Barriga, S.; Soliz, P.; MacCormick, I.; Taylor, T.; Harding, S.; Lewallen, S.; Joshi, V.

    2016-03-01

    Cerebral malaria (CM) is a life-threatening clinical syndrome associated with malarial infection. It affects approximately 200 million people, mostly sub-Saharan African children under five years of age. Malarial retinopathy (MR) is a condition in which lesions such as whitening and vessel discoloration that are highly specific to CM appear in the retina. Other unrelated diseases can present with symptoms similar to CM, therefore the exact nature of the clinical symptoms must be ascertained in order to avoid misdiagnosis, which can lead to inappropriate treatment and, potentially, death. In this paper we outline the first system to detect the presence of discolored vessels associated with MR as a means to improve the CM diagnosis. We modified and improved our previous vessel segmentation algorithm by incorporating the `a' channel of the CIELab color space and noise reduction. We then divided the segmented vasculature into vessel segments and extracted features at the wall and in the centerline of the segment. Finally, we used a regression classifier to sort the segments into discolored and not-discolored vessel classes. By counting the abnormal vessel segments in each image, we were able to divide the analyzed images into two groups: normal and presence of vessel discoloration due to MR. We achieved an accuracy of 85% with sensitivity of 94% and specificity of 67%. In clinical practice, this algorithm would be combined with other MR retinal pathology detection algorithms. Therefore, a high specificity can be achieved. By choosing a different operating point in the ROC curve, our system achieved sensitivity of 67% with specificity of 100%.

  2. RESISTÊNCIA DO VIMEIRO, Salix spp. (Salicaceae, À PRAGAS E DOENÇAS

    Directory of Open Access Journals (Sweden)

    Pedro Boff

    2007-03-01

    Full Text Available Este trabalho teve o objetivo de avaliar a reação de resistência a pragas e doenças de clones locais do vimeiro comum, Salix x rubens, e de espécies introduzidas da Argentina, Chile, Alemanha e França. Três experimentos foram conduzidos em campo no ciclo de cultivo 2003/2004, em diferentes ecossistemas do Planalto Serrano Catarinense. As avaliações de resistência constaram da estimativa de danos causados pelos insetos e da severidade de doenças foliares. No conjunto de todas as avaliações, os acessos – genótipos introduzidos – EEL_10 (Salix nigra, EEL_08 (Salix sp., EEL_02 (Salix x rubens, EEL_13 (Salix sp., EEL_09 (Salix alba, e EEL_20 (Salix sp. foram os mais resistentes ao ataque de pragas e doenças. Dada a reação diferenciada dos acessos quanto ao ataque de doenças e pragas, as próximas ações de pesquisa devem proceder aos testes de artesanato para verificar a sua adequação à demanda para confecção de peças finas.

  3. Does SO{sub 2} fumigation change the chemical defense of woody plants: the effect of short-term SO{sub 2} fumigation on the metabolism of deciduous Salix Myrsinifolia plants

    Energy Technology Data Exchange (ETDEWEB)

    Julkunen-Tiitto, R.; Lavola, A.; Kainulainen, P. [University of Joensuu, Joensuu (Finland). Dept. of Biology

    1995-08-01

    The effect of a moderate increase in atmospheric sulfur dioxide on the production of phenolic secondary chemicals, soluble sugars and phytomass distribution within plants was investigated in six willow ({ital Salix myrsinifolia Salisb}) clones. The plants were cultivated for 3 weeks under 0.11 ppm of SO{sub 2} (300{mu}g m{sup -3}). The production of salicin and chlorogenic acid was significantly reduced under increased SO{sub 2}. However, salicortin, 2{prime}-O-acetylsalicortin, (+)-catechin and two unknown phenolics did not show any clear trend. The increase in SO{sub 2} did not affect the glucose, fructose and sucrose contents. The final weight of the SO{sub 2}-treatment plants was significantly greater than that of the control plants: the leaf, stem and root phytomass was from 14 to 48% greater under increased SO{sub 2}. All the clones showed the same trend, although there was a significant variation in phytomass production. Results indicate, although not consistently, that even a short-term exposure of enhanced atmospheric SO{sub 2} may change moderately the accumulation pattern of willow phenolics. 20 refs., 2 tabs.

  4. Combined enhancements of temperature and UVB influence growth and phenolics in clones of the sexually dimorphic Salix myrsinifolia

    Energy Technology Data Exchange (ETDEWEB)

    Nybakken, L.; Hoerkkae, R.; Julkunen-Tiitto, R. (Univ. of Eastern Finland. Dept. of Biology, Joensuu (Finland))

    2012-08-15

    Although several climatic factors are expected to change simultaneously in the future, the effect of such combined changes on plants have seldom been tested under field conditions. We report on a field experiment with dark-leaved willow, Salix myrsinifolia, subjected to enhancements in ultraviolet-A (UVA), UVB radiation and temperature, setup in Joensuu, Eastern Finland. S. myrsinifolia is a dioecious species, known as an important food plant for many herbivores. Cuttings of eight clones, four of each sex, of dark-leaved willow were planted in the field in spring 2009. In both 2009 and 2010, the total biomass increased significantly with temperature, and in 2010 there was an additive effect of UVB radiation. Both height and diameter increased with temperature in 2009, while the effect on height growth ceased in 2010. Males had greater diameter growth than females in 2010. Most phenolic compounds in the leaves decreased under enhanced temperature in both growing seasons. In 2010, four of six salicylates increased in response to enhanced temperature. Some quercetin derivatives increased under enhanced UVB radiation. Females had higher concentrations of chlorogenic acids than males, and while enhanced temperature reduced chlorogenic acid in females only, luteolins were reduced only in males. In summary, the combined enhancements gave no effects in addition to those that appeared under the single-factor treatments, except for the additive effect of UVB on temperature-increased biomass. The few gender-related differences found in response to climate change do not allow any marked expectations of future climate-induced changes in sex ratios. (Author)

  5. Salix transect of Europe: variation in ploidy and genome size in willow-associated common nettle, Urtica dioica L. sens. lat., from Greece to arctic Norway.

    Science.gov (United States)

    Cronk, Quentin; Hidalgo, Oriane; Pellicer, Jaume; Percy, Diana; Leitch, Ilia J

    2016-01-01

    The common stinging nettle, Urtica dioica L. sensu lato, is an invertebrate "superhost", its clonal patches maintaining large populations of insects and molluscs. It is extremely widespread in Europe and highly variable, and two ploidy levels (diploid and tetraploid) are known. However, geographical patterns in cytotype variation require further study. We assembled a collection of nettles in conjunction with a transect of Europe from the Aegean to Arctic Norway (primarily conducted to examine the diversity of Salix and Salix -associated insects). Using flow cytometry to measure genome size, our sample of 29 plants reveals 5 diploids and 24 tetraploids. Two diploids were found in SE Europe (Bulgaria and Romania) and three diploids in S. Finland. More detailed cytotype surveys in these regions are suggested. The tetraploid genome size (2C value) varied between accessions from 2.36 to 2.59 pg. The diploids varied from 1.31 to 1.35 pg per 2C nucleus, equivalent to a haploid genome size of c. 650 Mbp. Within the tetraploids, we find that the most northerly samples (from N. Finland and arctic Norway) have a generally higher genome size. This is possibly indicative of a distinct population in this region.

  6. Phytoextraction with Salix viminalis in a moderately to strongly contaminated area.

    Science.gov (United States)

    Tőzsér, Dávid; Harangi, Sándor; Baranyai, Edina; Lakatos, Gyula; Fülöp, Zoltán; Tóthmérész, Béla; Simon, Edina

    2018-02-01

    We tested the suitability of Salix viminalis for phytoextraction with the analysis of selected elements in soil, root, and leaf, and by visual tree condition assessment in an area with varying levels of contamination. Bioconcentration factor (BCF) and translocation factor (TF) were used to assess the phytoextraction potential of willows. The middle part of the study area was strongly contaminated, while the northern and southern parts were moderately contaminated. We found increasing element concentrations toward deeper layers. Mean concentrations of elements in roots were similar among the three parts, while in leaves the highest concentrations were found in the strongly contaminated part of the study area. Tree condition scores were the lowest in the strongly contaminated part of the study area, which was caused by Al, Ca, K, Mg, Ni, Sr, and Zn concentration. These elements induced leaf disease and leaf feeders. The highest BCF values were found for Cu, Fe, Mn, and Zn in root, and for Cd and Zn in leaves, indicating that S. viminalis had high accumulation potential of these elements. Furthermore, TF values were high for Cd, Mn, Sr, and Zn. Our results also demonstrated that soil element composition has major influence on the condition of S. viminalis individuals. Furthermore, visual condition assessment was found to be a useful tool to assess the phytoextraction potential of trees.

  7. Analysis of genetic diversity in female, male and half sibs willow ...

    African Journals Online (AJOL)

    The genetic diversity among genotypes of Salix sp. was found to be high, suggesting the importance and feasibility of introducing elite genotypes from different origins for Salix germplasm conservation and breeding programs. Keywords: Salix sp., half sibs, molecular markers, genomic DNA fingerprinting. African Journal of ...

  8. Discoloration of plasticized PVC upon irradiation

    International Nuclear Information System (INIS)

    Kojima, Keiichi; Ueno, Keiji; Kumafuji, Hisao.

    1981-01-01

    The effects of the factors on the discoloration of PVC cross-linked by electron irradiation, such as irradiation dose, the polymerization degree of PVC resin, plasticizers and stabilizers, were studied. The composition of the plasticized PVC used for the experiment was 100 PHR of PVC, 50 PHR of plasticizer, 5 PHR of stabilizer and 5 PHR of cross-linking agent (TMPMA). Three samples with the different degree of polymerization of the PVC resin were used, namely 750, 1050 and 2600. As the plasticizers, phthalic acid esters (DBP, DOP, DIDP), trimellitic acid esters (TOTM, n-TOTM), fatty acid esters (DOS, DOZ), polyester and epoxy group plasticizers were used. The irradiation dose for the test was 3, 6 and 12 Mrad. The experimental results are summarized as follows. As the electron irradiation dose was higher, the resultant discoloration was more remarkable, and the optimum irradiation dose was below 6 Mrad. The degree of polymerization of the PVC resin did not affect the irradiation discoloration. However it was noticed that the cross-linking efficiency was better as the degree of polymerization was higher. The cross-linking efficiency was better as the content of plasticizer was more. The fatty acid esters and epoxy groups showed less discoloration and better cross-linking efficiency. Tin and barium-zinc stabilizers were good. (Kako, I.)

  9. The effect of air pollution and other environmental stressors on leaf fluctuating asymmetry and specific leaf area of Salix alba L

    Energy Technology Data Exchange (ETDEWEB)

    Wuytack, Tatiana, E-mail: tatiana.wuytack@ua.ac.be [Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp (Belgium); Wuyts, Karen, E-mail: karen.wuyts@ugent.be [Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp (Belgium); Laboratory of Forestry, Department of Forest and Water Management, Ghent University, Geraardsbergsesteenweg 267, B-9090 Gontrode (Melle) (Belgium); Van Dongen, Stefan, E-mail: stefan.vandongen@ua.ac.be [Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp (Belgium); Baeten, Lander, E-mail: lander.baeten@ugent.be [Laboratory of Forestry, Department of Forest and Water Management, Ghent University, Geraardsbergsesteenweg 267, B-9090 Gontrode (Melle) (Belgium); Kardel, Fatemeh, E-mail: fatemeh.kardel@ua.ac.be [Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp (Belgium); Verheyen, Kris, E-mail: kris.verheyen@ugent.be [Laboratory of Forestry, Department of Forest and Water Management, Ghent University, Geraardsbergsesteenweg 267, B-9090 Gontrode, Melle (Belgium); Samson, Roeland, E-mail: roeland.samson@ua.ac.be [Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp (Belgium)

    2011-10-15

    We aimed at evaluating the effect of low-level air pollution on leaf area fluctuating asymmetry (FAA) and specific leaf area (SLA) of Salix alba L., taking into account other environmental factors. Cuttings were grown in standardized conditions in the near vicinity of air quality measuring stations in Belgium. Variability of SLA and FAA between measuring stations explained 83% and 7.26%, respectively, of the total variability. FAA was not influenced by air pollution or environmental factors such as shading, herbivory, air temperature and humidity. SLA was increased by an increase in shadow, while NO{sub x} and O{sub 3} concentrations had only a marginal influence. The influence of SO{sub 2} concentration was negligible. Although our data analysis suggests a relationship between SLA and NO{sub x}/O{sub 3} concentration, the absence of a straightforward relationship between FAA and SLA and air pollution still questions the usefulness of these bio-indicators for monitoring air pollution. - Highlights: > Leaf characteristics of white willow as possible bio-indicators for air quality. > Fluctuating asymmetry is not a good bio-indicator for monitoring the air quality. > Shadow increases specific leaf area. > NO{sub x} and O{sub 3} change specific leaf area of white willow. - Specific leaf area of S. alba increased with increasing shade and, in less extent, with increasing NO{sub x} and decreasing O{sub 3} concentration, while leaf asymmetry did not respond to air pollution

  10. The effect of air pollution and other environmental stressors on leaf fluctuating asymmetry and specific leaf area of Salix alba L

    International Nuclear Information System (INIS)

    Wuytack, Tatiana; Wuyts, Karen; Van Dongen, Stefan; Baeten, Lander; Kardel, Fatemeh; Verheyen, Kris; Samson, Roeland

    2011-01-01

    We aimed at evaluating the effect of low-level air pollution on leaf area fluctuating asymmetry (FAA) and specific leaf area (SLA) of Salix alba L., taking into account other environmental factors. Cuttings were grown in standardized conditions in the near vicinity of air quality measuring stations in Belgium. Variability of SLA and FAA between measuring stations explained 83% and 7.26%, respectively, of the total variability. FAA was not influenced by air pollution or environmental factors such as shading, herbivory, air temperature and humidity. SLA was increased by an increase in shadow, while NO x and O 3 concentrations had only a marginal influence. The influence of SO 2 concentration was negligible. Although our data analysis suggests a relationship between SLA and NO x /O 3 concentration, the absence of a straightforward relationship between FAA and SLA and air pollution still questions the usefulness of these bio-indicators for monitoring air pollution. - Highlights: → Leaf characteristics of white willow as possible bio-indicators for air quality. → Fluctuating asymmetry is not a good bio-indicator for monitoring the air quality. → Shadow increases specific leaf area. → NO x and O 3 change specific leaf area of white willow. - Specific leaf area of S. alba increased with increasing shade and, in less extent, with increasing NO x and decreasing O 3 concentration, while leaf asymmetry did not respond to air pollution

  11. Resistência do vimeiro, Salix spp. (Salicaceae, à pragas e doenças.

    Directory of Open Access Journals (Sweden)

    Jamille Casa

    2010-08-01

    Full Text Available Este trabalho teve o objetivo de avaliar a reação de resistência a pragas e doenças de clones locais do vimeiro comum, Salix x rubens, e de espécies introduzidas da Argentina, Chile, Alemanha e França. Três experimentos foram conduzidos em campo no ciclo de cultivo 2003/2004, em diferentes ecossistemas do Planalto Serrano Catarinense. As avaliações de resistência constaram da estimativa de danos causados pelos insetos e da severidade de doenças foliares. No conjunto de todas as avaliações, os acessos - genótipos introduzidos - EEL_10 (Salix nigra, EEL_08 (Salix sp., EEL_02 (Salix x rubens, EEL_13 (Salix sp., EEL_09 (Salix alba, e EEL_20 (Salix sp. foram os mais resistentes ao ataque de pragas e doenças. Dada a reação diferenciada dos acessos quanto ao ataque de doenças e pragas, as próximas ações de pesquisa devem proceder aos testes de artesanato para verificar a sua adequação à demanda para confecção de peças finas.

  12. Clinical Characteristics of Abutment Teeth with Gingival Discoloration.

    Science.gov (United States)

    Ristic, Ljubisa; Dakovic, Dragana; Postic, Srdjan; Lazic, Zoran; Bacevic, Miljana; Vucevic, Dragana

    2017-04-06

    The grey-bluish discoloration of gingiva (known as "amalgam tattoo") does not appear only in the presence of amalgam restorations. It may also be seen in cases of teeth restored with cast dowels and porcelain-fused-to-metal (PFM) restorations. The aim of this article was to determine the clinical characteristics of abutment teeth with gingival discoloration. This research was conducted on 25 patients referred for cast dowel and PFM restorations. These restorations were manufactured from Ni-Cr alloys. Ninety days after cementing the fixed prosthodontic restorations, the abutment teeth (n = 61) were divided into a group with gingival discoloration (GD) (n = 25) and without gingival discoloration (NGD) (n = 36). The control group (CG) comprised the contralateral teeth (n = 61). Plaque index, gingival index, clinical attachment level, and probing depth were assessed before fabrication and also 90 days after cementation of the PFM restorations. The gingival index, clinical attachment level, and probing depths of the abutment teeth that had GD were statistically higher before restoration, in comparison with the abutment teeth in the NGD and control groups. Ninety days after cementation, the abutment teeth with GD had significantly lower gingival indexes and probing depths, compared to the abutment teeth in the NGD group. Both abutment teeth groups (GD and NGD) had significantly higher values of clinical attachment levels when compared to the control group. There were no statistically significant differences in plaque index values between the study groups. The results of this study indicated that impairment of periodontal status of abutment teeth seemed to be related to the presence of gingival discolorations. Therefore, fabrication of fixed prosthodontic restorations requires careful planning and abutment teeth preparation to minimize the occurrence of gingival discolorations. With careful preparation of abutment teeth for cast dowels and crown restorations it may be

  13. Willow wood production on radionuclide polluted areas

    Directory of Open Access Journals (Sweden)

    Rodkin Oleg I.

    2010-01-01

    Full Text Available ABSTRACT: One of the key environmental problems in Belarus is effective use of agricultural lands contaminated by radionuclide due to the Chernobyl disaster. The alternative method to traditional agricultural crops is fast growing willow cultivation. It is possible to use biomass of willow as renewable energy source. The goal of our investigation was the estimation of environmental aspects of willow wood production on polluted areas. The field study experiments (2007-2010 were conducted at Krichev district of Mogilev region in eastern Belarus. This region characterized by high level of Cs-137 contamination as well as high level of heavy metals pollution. In the first stage of experiments, the concentration of cesium-137 in different parts of willow biomass had been measured and transfer factor calculated. The measuring had been done for leaves, roots, and wood. To control cesium-137 accumulation in willow biomass we apply different types (nitrogen N, phosphorus P and potassium K and dose of fertilizer. The experiments show that potassium mineral fertilizer is the key factor for radionuclide accumulation control. The optimal dose of potassium is 90 kg per hectare. On the base of experimental results the model of cesium-137 accumulation in the wood for a 21 year has been developed. In accordance with calculation to the end of willow cultivation (21 year concentration of cesium-137 in wood will not be higher than permitted even with the level of cesium-137 contamination in the soil 1480 kBq/m2 (maximum 140 kqB/m2 with permitted level for firewood is 740 Bq/kg.. The concentration of cesium-137 in the roots increases gradually and get maximum in 21 year (3000 kqB/m2. Our results confirm that in the sum about 0.8 million hectares of radionuclide polluted arable lands partly excluded from agricultural practice in Belarus could be used for willow biomass production.

  14. Willow growing - Methods of calculation and profitability

    International Nuclear Information System (INIS)

    Rosenqvist, H.

    1997-01-01

    The calculation method presented here makes it possible to conduct profitability comparisons between annual and perennial crops and in addition take the planning situation into account. The method applied is a modified total step calculation. The difference between a traditional total step calculation and the modified version is the way in which payments and disbursements are taken into account over a period of several years. This is achieved by combining the present value method and the annuity method. The choice of interest rate has great bearing on the result in perennial calculations. The various components influencing the interest rate are analysed and factors relating to the establishment of the interest rate in different situations are described. The risk factor can be an important variable component of the interest rate calculation. Risk is also addressed from an approach in accordance with portfolio theory. The application of the methods sheds light on the profitability of Salix cultivation from the viewpoint of business economics, and also how different factors influence the profitability of Salix cultivation. Aspects studied are harvesting intervals, the importance of yield level, the competitiveness of Salix versus grain cultivation, the influence of income taxes on profitability etc. Methods for evaluation of activities concerning cultivation of a perennial crop are described and also involve the application of nitrogen fertilization to Salix cultivation. Studies have been performed using these methods to look into nitrogen fertilizer profitability in Salix cultivation during the first rotation period. Nitrogen fertilizer profitability has been investigated involving both production functions and cost calculations, taking the year fertilization into consideration. 72 refs., 2 figs., 52 tabs

  15. Biomass production in willows. What did we know before the energy crisis

    Energy Technology Data Exchange (ETDEWEB)

    Perttu, K L [ed.

    1984-12-01

    The biological foundations of biomass with willows originate in the experiences from basket willow husbandry. This was an established discipline in Europe in the 18th century. Problems concerning site preparation, selection of clones, planting as cuttings, spacing, weed control, rotation time, harvesting and coppicing vigour with respect to the longevity of the stand, were practically solved at the research level and already in practice. The yield potential of basket willow and willows for hoop production as well as yield figures from field experiments were quite high also according to present-day biomass willow experiments. An explanation of this could be the much higher stand densities than has been customary in current willow experiments. Although many practical questions got their answers in basket willow husbandry, open questions still remain. The basket willow era gave only little experience on willow production in peatlands; actually peatsoils were almost avoided. Knowledge of nutrient require ments and fertilization was also rather elementary. These aspects must therefore be established for biomass production. Control of weeds in the establishment phase of the willow husbandry was solved by manual work. Since this is a labour intensive method which is no longer possible, a more modern weed control needs to be developed for current husbandry. As a whole it is a task for related research to attach proper optimization of cultural techniques to suitable willow clones in order to attain and maintain as high a production level as was the case in the old basket willow husbandry. With 25 refs.

  16. Genetics of phenotypic plasticity and biomass traits in hybrid willows across contrasting environments and years.

    Science.gov (United States)

    Berlin, Sofia; Hallingbäck, Henrik R; Beyer, Friderike; Nordh, Nils-Erik; Weih, Martin; Rönnberg-Wästljung, Ann-Christin

    2017-07-01

    Phenotypic plasticity can affect the geographical distribution of taxa and greatly impact the productivity of crops across contrasting and variable environments. The main objectives of this study were to identify genotype-phenotype associations in key biomass and phenology traits and the strength of phenotypic plasticity of these traits in a short-rotation coppice willow population across multiple years and contrasting environments to facilitate marker-assisted selection for these traits. A hybrid Salix viminalis  × ( S. viminalis × Salix schwerinii ) population with 463 individuals was clonally propagated and planted in three common garden experiments comprising one climatic contrast between Sweden and Italy and one water availability contrast in Italy. Several key phenotypic traits were measured and phenotypic plasticity was estimated as the trait value difference between experiments. Quantitative trait locus (QTL) mapping analyses were conducted using a dense linkage map and phenotypic effects of S. schwerinii haplotypes derived from detected QTL were assessed. Across the climatic contrast, clone predictor correlations for biomass traits were low and few common biomass QTL were detected. This indicates that the genetic regulation of biomass traits was sensitive to environmental variation. Biomass QTL were, however, frequently shared across years and across the water availability contrast. Phenology QTL were generally shared between all experiments. Substantial phenotypic plasticity was found among the hybrid offspring, that to a large extent had a genetic origin. Individuals carrying influential S. schwerinii haplotypes generally performed well in Sweden but less well in Italy in terms of biomass production. The results indicate that specific genetic elements of S. schwerinii are more suited to Swedish conditions than to those of Italy. Therefore, selection should preferably be conducted separately for such environments in order to maximize biomass

  17. Effect of HALS on radiation discoloration of PE

    International Nuclear Information System (INIS)

    Wang Huiliang; Wang Chun; Chen Wenxiu

    2001-01-01

    The effects of hindered amine light stabilizers (HALS) on the radiation-induced discoloration of polyethylene (PE) are studied by measuring the yellowness index (YI) of PE. It is found that all the HALS used are effective in preventing PE from radiation-induced discoloration. The YI of PE added pentamethyl HALS (PMPM, PPMPM) is a little higher than that of PE added corresponding tetramethyl HALS (TMPM, PTMPM) when radiation dose is low than 100 kGy, but when the radiation dose is higher than 100 kGy, the YI of PE added pentamethyl HALS is lower. Pentamethyl HALS is more effective in preventing PE from radiation-induced discoloration when radiation dose is higher. It is also found that polymeric HALS is more effective in preventing PE from radiation-induced discoloration than corresponding monomeric HALS when radiation dose is higher than 200 kGy. The formation of alkyl free radical, carbonyl after irradiation is measured. It is found that the relative concentration of free radical formed in PE added TMPM is higher than that of PE added PMPM when radiation dose is relatively high. The carbonyl index of PE containing pentamethyl HALS is less than that of PE containing tetramethyl HALS when radiation is relatively low, but the results is contrary when radiation dose is relatively high. It is believed that HALS prevents PE from radiation-induced discoloration by scavenging free radicals formed in irradiated PE

  18. Bioenergy from willow. 1995 Annual report, November 1987--December 1995

    Energy Technology Data Exchange (ETDEWEB)

    White, E.H.; Abrahamson, L.P.

    1997-07-01

    Experiments were established at Tully, New York, by the State University of New York College of Environmental Science and Forestry, in cooperation with the University of Toronto and the Ontario Ministry of Natural Resources, to assess the potential of willows for wood biomass production. Specific objectives included determining the effects of clone type, fertilization, spacing, cutting cycle, and irrigation on biomass production. Production was high, with willow clone SV1 yielding nearly 32 oven dry tons per acre (odt ac{sup -1}) with three-year harvest cycle, irrigation, and fertilization. Clone type, fertilization, spacing, cutting cycle, and irrigation all significantly affected biomass production. Willow clone-site trials planted at Massena, and Tully, NY in 1993 grew well during 1994 and 1995, but some clones in the Massena trial were severely damaged by deer browse. Several new cooperators joined the project, broadening the funding base, and enabling establishment of additional willow plantings. Willow clone-site trials were planted at Himrod, King Ferry, Somerset, and Tully, NY, during 1995. A willow cutting orchard was planted during 1995 at the NYS Department of Environmental Conservation Saratoga Tree Nursery in Saratoga, NY. Plans are to begin site preparation for a 100+ acre willow bioenergy demonstration farm in central New York, and additional clone-site trials, in 1996.

  19. Willow bioenergy plantation research in the Northeast

    Energy Technology Data Exchange (ETDEWEB)

    White, E.H.; Abrahamson, L.P.; Kopp, R.F. [SUNY College of Environmental Science and Forestry, Syracuse, NY (United States); Nowak, C.A. [USDA Forest Service, Warren, PA (United States)

    1993-12-31

    Experiments were established in Central New York in the spring of 1987 to evaluate the potential of Salix for biomass production in bioenergy plantations. Emphasis of the research was on developing and refining establishment, tending and maintenance techniques, with complimentary study of breeding, coppice physiology, pests, nutrient use and bioconversion to energy products. Current yields utilizing salix clones developed in cooperation with the University of Toronto in short-rotation intensive culture bioenergy plantations in the Northeast approximate 8 oven dry tons per acre per year with annual harvesting. Successful clones have been identified and culture techniques refined. The results are now being integrated to establish a 100 acre Salix large-scale bioenergy farm to demonstrate current successful biomass production technology and to provide plantations of sufficient size to test harvesters; adequately assess economics of the systems; and provide large quantities of uniform biomass for pilot-scale conversion facilities.

  20. Novel Field Data on Phytoextraction: Pre-Cultivation With Salix Reduces Cadmium in Wheat Grains.

    Science.gov (United States)

    Greger, Maria; Landberg, Tommy

    2015-01-01

    Cadmium (Cd) is a health hazard, and up to 43% of human Cd intake comes from wheat products, since Cd accumulates in wheat grains. Salix spp. are high-accumulators of Cd and is suggested for Cd phytoextraction from agricultural soils. We demonstrate, in field, that Salix viminalis can remove Cd from agricultural soils and thereby reduce Cd accumulation in grains of wheat subsequently grown in a Salix-treated field. Four years of Salix cultivation reduce Cd concentration in the soil by up to 27% and in grains of the post-cultivated wheat by up to 33%. The higher the plant density of the Salix, the greater the Cd removal from the soil and the lower the Cd concentration in the grains of post-cultivated wheat, the Cd reduction remaining stable several years after Salix cultivation. The effect occurred in both sandy and clayey soil and in winter and spring bread wheat cultivars. Already one year of Salix cultivation significantly decrease Cd in post grown wheat grains. With this field experiment we have demonstrated that phytoextraction can reduce accumulation of a pollutant in post-cultivated wheat and that phytoextraction has no other observed effect on post-cultivated crops than reduced uptake of the removed pollutant.

  1. Salix purpurea Stimulates the Expression of Specific Bacterial Xenobiotic Degradation Genes in a Soil Contaminated with Hydrocarbons.

    Directory of Open Access Journals (Sweden)

    Antoine P Pagé

    Full Text Available The objectives of this study were to uncover Salix purpurea-microbe xenobiotic degradation systems that could be harnessed in rhizoremediation, and to identify microorganisms that are likely involved in these partnerships. To do so, we tested S. purpurea's ability to stimulate the expression of 10 marker microbial oxygenase genes in a soil contaminated with hydrocarbons. In what appeared to be a detoxification rhizosphere effect, transcripts encoding for alkane 1-monooxygenases, cytochrome P450 monooxygenases, laccase/polyphenol oxidases, and biphenyl 2,3-dioxygenase small subunits were significantly more abundant in the vicinity of the plant's roots than in bulk soil. This gene expression induction is consistent with willows' known rhizoremediation capabilities, and suggests the existence of S. purpurea-microbe systems that target many organic contaminants of interest (i.e. C4-C16 alkanes, fluoranthene, anthracene, benzo(apyrene, biphenyl, polychlorinated biphenyls. An enhanced expression of the 4 genes was also observed within the bacterial orders Actinomycetales, Rhodospirillales, Burkholderiales, Alteromonadales, Solirubrobacterales, Caulobacterales, and Rhizobiales, which suggest that members of these taxa are active participants in the exposed partnerships. Although the expression of the other 6 marker genes did not appear to be stimulated by the plant at the community level, signs of additional systems that rest on their expression by members of the orders Solirubrobacterales, Sphingomonadales, Actinomycetales, and Sphingobacteriales were observed. Our study presents the first transcriptomics-based identification of microbes whose xenobiotic degradation activity in soil appears stimulated by a plant. It paints a portrait that contrasts with the current views on these consortia's composition, and opens the door for the development of laboratory test models geared towards the identification of root exudate characteristics that limit the

  2. Environmental influence of willow coppice systems on farm land

    International Nuclear Information System (INIS)

    Ledin, S.

    1997-01-01

    As for all cultivated crops, there are numerous environmental influences when growing willows. These influences are connected with additions to the soil, management measures and changes caused by the developing crop. As a perennial crop with good root penetration into the soil, high water use and efficient nutrient uptake over a long growing season, short rotation forests with willows have an influence on the environment that differs from the influence of annual farm crops. Also the landscape is affected differently by the dense willow stand with a height of seven meters before harvest. There is usually less use of herbicides in the perennial woody crop. Due to larger amounts of litter from the willow plantation and no tillage, there is with time an increase of the humus content in the soil. In relation to this, the soil fauna, using organic material for food, prosper in the short rotation forest. Considerably more cadmium is taken up by the willows than by conventional annual crops. This could be used to clean the soil of cadmium, but more knowledge is needed for the optimisation of this procedure. Willows are usually efficient in taking up nutrients, including nitrogen, but when used as vegetation filters it is important to assess the actual ability of a stand to take up N. This ability varies considerably with the development stage of the plantation and also with site conditions. (au) 30 refs

  3. Genetic variation for characters of importance for growth in Salix viminalis L. Final report; Genetisk variation foer karaktaerer av betydelse foer tillvaext hos Salix viminalis L. Slutrapport

    Energy Technology Data Exchange (ETDEWEB)

    Roennberg-Waestljung, Ann Christin; Gullberg, Urban [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Plant Biology

    2000-04-01

    The overall goal for this project was to study the genetic variation and the genetic relationships for different growth characters and for water use efficiency (WUE) in Salix viminalis and also to use this knowledge to formulate breeding goals for Salix. Two factorial crossings with Swedish and Polish origin, each with 320 families have been used. Part of the Polish material was used to study the genetic variation for carbon isotope quota. Carbon isotope quota gives a measure of the WUE for the plant. Crossings have been made to change and improve the WUE in Salix viminalis. Construction of a genetic linkage map has started and the map can be used to identify genetic markers for WUE. The results show that most of the growth characters have both additive genetic variation and also a high degree of dominance genetic variation. A strategy in the breeding where both additive and dominance variation can be utilized should be adopted. WUE show mainly additive genetic variation but also a high heritability. This gives great opportunities to improve Salix material for WUE through recurrent selection.

  4. Effects of contaminated soil on the growth performance of young Salix (Salix schwerinii E. L. Wolf) and the potential for phytoremediation of heavy metals.

    Science.gov (United States)

    Salam, Mir Md Abdus; Kaipiainen, Erik; Mohsin, Muhammad; Villa, Aki; Kuittinen, Suvi; Pulkkinen, Pertti; Pelkonen, Paavo; Mehtätalo, Lauri; Pappinen, Ari

    2016-12-01

    Salix schwerinii was tested in a pot experiment to assess plant growth performance i.e., relative height and dry biomass and the potential for heavy metal uptake in soils polluted with chromium, zinc, copper, nickel and total petroleum hydrocarbons. The soil used in the pot experiment was collected from a landfill area in Finland. Peat soil was added at different quantities to the polluted soil to stimulate plant growth. The plants were irrigated with tap water or processed water (municipal waste water) to further investigate the effects of nutrient loading on plant biomass growth. The soil was treated at two pH levels (4 and 6). The results showed that the addition of 40-70% peat soil at pH 6 to a polluted soil, and irrigation with processed water accelerated plant growth and phytoextraction efficiency. In the pot experiment, Salix grown in chromium, zinc, copper, nickel and total petroleum hydrocarbons -contaminated field soil for 141 days were unaffected by the contaminated soil and took up excess nutrients from the soil and water. Total mean chromium concentration in the plant organs ranged from 17.05 to 250.45 mg kg -1 , mean zinc concentration ranged from 142.32 to 1616.59 mg kg -1 , mean copper concentration ranged from 12.11 to 223.74 mg kg -1 and mean nickel concentration ranged from 10.11 to 75.90 mg kg -1 . Mean chromium concentration in the plant organs ranged from 46 to 94%, mean zinc concentration ranged from 44 to 76%, mean copper concentration ranged from 19 to 54% and mean nickel concentration ranged from 8 to 21% across all treatments. Under the different treatments, chromium was taken up by Salix in the largest quantities, followed by zinc, copper and nickel respectively. Salix also produced a moderate reduction in total petroleum total petroleum hydrocarbons in the polluted soil. The results from the pot experiment suggest that Salix schwerinii has the potential to accumulate significant amounts of chromium, zinc, copper and nickel

  5. Carbon isotope variation in shrub willow (Salix spp.) ring-wood as an indicator of long-term water status, growth and survival

    International Nuclear Information System (INIS)

    Schifman, Laura A.; Stella, John C.; Volk, Timothy A.; Teece, Mark A.

    2012-01-01

    Quantifying interannual change in water status of woody plants using stable carbon isotopes provides insight on long-term plant ecophysiology and potential success in variable environments, including under-utilized agricultural land for biomass production and highly disturbed sites for phytoremediation applications. We analyzed δ 13 C values in annual ring-wood of four shrub willow varieties used for biomass production and phytoremediation at three sites in central New York State (U.S.A). We tested a cost-effective sampling method for estimating whole-shrub water status by comparing δ 13 C values of the plant’s largest stem against a composite sample of all stems. The largest stem showed 0.3‰ 13 C enrichment (range −0.7–1.1‰) compared to the whole-plant, making it a more sensitive indicator of water status than the composite sample. Growing season precipitation exerted a strong negative influence on wood tissue chemistry, with an average 0.26‰ 13 C depletion per 100 mm increase in precipitation. An average annual 0.28‰ 13 C enrichment was also observed with increased plant age; this pattern was consistent among all four willow varieties and across sites. Finally, increased 13 C enrichment in wood tissue was positively associated with plant size at the individual plant level, and associated negatively and more variably survival at the plot scale. These results have important implications for the design and management of biomass production and phytoremediation systems. Increased sensitivity of older plants suggests that longer rotations may experience growth limitations and/or lower survival in low-precipitation years, resulting in reduced yields of biomass crops and loss of effectiveness in phytoremediation applications. -- Highlights: ► A 0.26‰ 13 C depletion in wood tissue occurred per 100 mm increase in precipitation. ► There was an average 13 C enrichment with plant age and size for all varieties. ► Greater 13 C enrichment often lead to

  6. Performance of Salix viminalis and Populus nigra x Populus maximowiczii in short rotation intensive culture under high irrigation

    Energy Technology Data Exchange (ETDEWEB)

    Fillion, Maud; Brisson, Jacques [Departement de Sciences biologiques, Universite de Montreal, C.P. 6128, succ. Centre-ville, Montreal, Quebec (Canada); Institut de recherche en biologie vegetale, 4101 Sherbrooke East, Montreal, Quebec (Canada); Teodorescu, Traian I.; Labrecque, Michel [Institut de recherche en biologie vegetale, 4101 Sherbrooke East, Montreal, Quebec (Canada); Sauve, Sebastien [Departement de chimie, Universite de Montreal, C.P. 6128, succursale Centre-ville, Montreal, Quebec (Canada)

    2009-09-15

    On a plantation established in 2004 from stem cuttings at a density of 20,000 trees per hectare, we investigated growth and nutritional plant response to a high hydraulic regime for two species (Salix viminalis and Populus nigra x Populus maximowiczii), using a comparative approach with measurements from irrigated and control plots. The plantation was irrigated from June to September 2005 with about 140 mm per day. The equivalent of 120 Kg NO{sub 3}-N, 40 Kg P{sub 2}O{sub 5}-P and 85 Kg K{sub 2}O-K per hectare per year was applied by means of irrigation with wastewater. No mortality occurred and stem biomass production of both poplar and willow species were not statistically different on irrigated and control areas. However, S. viminalis revealed to be more tolerant to flooded conditions since these corresponded more closely to its nutritional requirements (foliar concentration of 20 mgN g{sup -1}). The capacity of S. viminalis to withstand waterlogged conditions could play an important role in the sustainability of a plantation for the filtration of effluent at low pollutant concentration. (author)

  7. 78 FR 41499 - Endangered and Threatened Wildlife and Plants; Threatened Status for the Northern Mexican...

    Science.gov (United States)

    2013-07-10

    ...), willows (Salix ssp.), canyon grape (Vitis arizonica), blackberry (Rubus ssp.), Arizona sycamore (Platanus... (Quercus gambelii), and ponderosa pine (Pinus ponderosa) (Rosen and Schwalbe 1988, pp. 34- 35). Rosen and...

  8. Phytoremediation potential of willow tress for aquifers contaminated with ethanol-blended gasoline

    Energy Technology Data Exchange (ETDEWEB)

    Corseuil, H.X. [Universidade Federal de Santa Catarina, Florianopolis (Brazil). Departamento de Engenharia Sanitaria e Ambiental; Moreno, F.N. [Universidade do Sul de Santa Catarina, Palhoca (Brazil). Centro de Ciencias Agrarias e das Engenharias

    2001-07-01

    Ethanol-blended gasoline has been used in Brazil for 20 years and, probably, is going to be more widely used in North America due to the MtBE environmental effects on groundwater. The potential impacts caused by the presence of ethanol in UST spills are related to the co-solvency effect and the preferential degradation of ethanol over the BTEX compounds. These interactions may increase the length of dissolved hydrocarbon plumes and the costs associated with site remediation. This study investigates the advantages of phytoremediation to overcome the problems associated with the presence of ethanol in groundwater contaminated with gasoline-ethanol mixtures. Experiments were performed under lab conditions with cuttings of Willow tree (Salix babylonica) cultivated hydroponically. Results showed that the cuttings were able to reduce ethanol and benzene concentrations by more than 99% in less than a week. The uptake of both contaminants was confirmed by blank controls and was significantly related to cuttings transpiration capacity. Sorption onto roots biomass also markedly affected the behavior of contaminants in solution. Experiments to evaluate plants' toxicity to ethanol indicated that plants were only affected when aqueous ethanol concentration reached 2000mgl{sup -1}. Results suggest that phytoremediation can be a good complement to intrinsic remediation in shallow aquifer sites contaminated with ethanol-blended gasoline spills. (Author)

  9. Diversity of cuticular wax among Salix species and Populus species hybrids.

    Science.gov (United States)

    Cameron, Kimberly D; Teece, Mark A; Bevilacqua, Eddie; Smart, Lawrence B

    2002-08-01

    The leaf cuticular waxes of three Salix species and two Populus species hybrids, selected for their ability to produce high amounts of biomass, were characterized. Samples were extracted in CH(2)Cl(2) three times over the growing season. Low kV SEM was utilized to observe differences in the ultrastructure of leaf surfaces from each clone. Homologous series of wax components were classified into organic groups, and the variation in wax components due to clone, sample time, and their interaction was identified. All Salix species and Populus species hybrids showed differences in total wax load at each sampling period, whereas the pattern of wax deposition over time differed only between the Salix species. A strong positive relationship was identified between the entire homologous series of alcohols and total wax load in all clones. Similarly strong relationships were observed between fatty acids and total wax load as well as fatty acids and alcohols in two Salix species and one Populus species hybrid. One Salix species, S. dasyclados, also displayed a strong positive relationship between alcohols and alkanes. These data indicate that species grown under the same environmental conditions produce measurably different cuticular waxes and that regulation of wax production appears to be different in each species. The important roles cuticular waxes play in drought tolerance, pest, and pathogen resistance, as well as the ease of wax extraction and analysis, strongly suggest that the characteristics of the cuticular wax may prove to be useful selectable traits in a breeding program.

  10. Status, ecology, and conservation of the southwestern willow flycatcher

    Science.gov (United States)

    Deborah M. Finch; Scott H. Stoleson

    2000-01-01

    This publication was prepared in response to a need expressed by southwestern agencies and organizations for a comprehensive assessment of the population status, history, biology, ecology, habitats, threats, and conservation of the southwestern willow flycatcher (Empidonax traillii extimus). The southwestern willow flycatcher was federally listed as...

  11. Early Studies on Protoplast Isolation of Ludisia discolor, A Wild Orchid.

    Science.gov (United States)

    Poobathy, Ranjetta; Zakaria, Rahmad; Hamzah, Syed Mohd Edzham Syed; Subramaniam, Sreeramanan

    2016-11-01

    The terrestrial Ludisia discolor , also referred to as the jewel orchid is prized for the quality of its leaves. L. discolor is known as a medicinal herb and is touted for its heat- and pathogen-resisting qualities. L. discolor is valuable in the production of both flavonoids and anthocyanins, antioxidants that are exalted in the health industry. Plant cell cultures have emerged as alternative sources of anthocyanin production. Plant protoplast cultures are used frequently in transient gene expression studies and in the establishment of callus and cell suspension cultures. Benefits of plant protoplast system include similarity to cells found in plant tissues, reproduction under controlled conditions, and prevention of masking of stress responses to previous handling techniques. A study was conducted to assess the amenability of the stem and leaves of L. discolor to protoplast isolation. The stem and leaf segments were weighed, sliced into thin layers, immersed in a digestion medium, washed and then cultured onto a recovery medium. Results indicated that the production of plant protoplasts from L. discolor may be viewed as an alternative in the generation of cell cultures and ultimately in the production of anthocyanins from the cell cultures.

  12. Biological diversity of Salix taxa in Cu, Pb and Zn phytoextraction from soil.

    Science.gov (United States)

    Mleczek, Mirosław; Rutkowski, Paweł; Goliński, Piotr; Kaczmarek, Zygmunt; Szentner, Kinga; Waliszewska, Bogusława; Stolarski, Mariusz; Szczukowski, Stefan

    2017-02-01

    The aim of the study was to estimate the efficiency of copper (Cu), lead (Pb) and zinc (Zn) phytoextraction by 145 Salix taxa cultivated in an area affected by industrial activity. Survivability and biomass of plants were also analyzed. The highest Cu, Pb and Zn content in shoots was 33.38 ± 2.91 (S. purpurea × viminalis 8), 24.64 ± 1.97 (S. fragilis 1) and 58.99 ± 4.30 (S. eriocephala 7) mg kg -1 dry weight, respectively. In the case of unwashed leaves, the highest content of these metals was 135.06 ± 8.14 (S. purpurea 26), 67.98 ± 5.27 (S. purpurea 45) and 142.56 ± 12.69 (S. alba × triandra 2) mg kg -1 dw, while in washed leaves it was 106.02 ± 11.12 (S. purpurea 45), 55.06 ± 5.75 (S. purpurea 45) and 122.87 ± 12.33 (S. alba × triandra 2) mg kg -1 dw, respectively. The differences between the highest and lowest values for Cu, Pb and Zn were 545%, 20500% and 535% in shoots; 2692%, 2560% and 7500% in unwashed leaves; and 3286%, 2221% and 6950% in washed leaves, respectively. S. acutifolia was able to effectively accumulate all three metals jointly, producing shoots that were well developed in both length and diameter when compared with the other tested willows-an ability that would suggest its high suitability for practical application.

  13. Local variability in growth and reproduction of Salix arctica in the High Arctic

    Directory of Open Access Journals (Sweden)

    Noémie Boulanger-Lapointe

    2016-06-01

    Full Text Available Arctic terrestrial ecosystems are heterogeneous because of the strong influences of microtopography, soil moisture and snow accumulation on vegetation distribution. The interaction between local biotic and abiotic factors and global climate patterns will influence species responses to climate change. Salix arctica (Arctic willow is a structuring species, ubiquitous and widespread, and as such is one of the most important shrub species in the High Arctic. In this study, we measured S. arctica reproductive effort, early establishment, survival and growth in the Zackenberg valley, north-east Greenland. We sampled four plant communities that varied with respect to snow conditions, soil moisture, nutrient content and plant composition. We found large variability in reproductive effort and success with total catkin density ranging from 0.6 to 66 catkins/m2 and seedling density from <1 to 101 seedlings/m2. There were also major differences in crown area increment (4–23 cm2/year and stem radial growth (40–74 µm/year. The snowbed community, which experienced a recent reduction in snow cover, supported young populations with high reproductive effort, establishment and growth. Soil nutrient content and herbivore activity apparently did not strongly constrain plant reproduction and growth, but competition by Cassiope tetragona and low soil moisture may inhibit performance. Our results show that local environmental factors, such as snow accumulation, have a significant impact on tundra plant response to climate change and will affect the understanding of regional vegetation response to climate change.

  14. 7 CFR 51.770 - Discoloration.

    Science.gov (United States)

    2010-01-01

    ... of a light shade of golden brown caused by rust mite or other means. Lighter shades of discoloration... other means may detract from the appearance of the fruit to a greater extent than the shade and amount... Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing...

  15. Schorl: a novel catalyst in mineral-catalyzed Fenton-like system for dyeing wastewater discoloration.

    Science.gov (United States)

    Xu, Huan-Yan; Prasad, Murari; Liu, Yu

    2009-06-15

    Mineral-catalyzed Fenton-like system has been found to be effective for the discoloration of dyeing wastewater. In our present study, schorl has been successfully developed as a novel heterogeneous catalyst for discoloration of an active commercial dye, Argazol blue (BF-BR), in an aqueous solution. Through a number of batch discoloration experiments under various conditions, it was found that the reactivity of the system increased by, respectively, increasing schorl dosage, temperature, hydrogen peroxide starting concentration and by decreasing the pH. At the condition of pH 2, T=55 degrees C, [BF-BR](0)=200mg/L, [H(2)O(2)](0)=48.5 mmol/L and schorl dosage=10 g/L, 100% of discoloration ratio can be achieved in less than 4 min, and 72% of total organic carbon (TOC) can be removed in less than 200 min. The reaction kinetics analysis shows that the discoloration of BF-BR follows the first-order kinetics. The schorl samples after BF-BR discoloration was tested by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Scanning electron microscope (SEM) and the results rule out the possibility of formation of new solid phases during BF-BR discoloration. The content of Fe ion leaching in the solution was also measured using inductively coupling plasma-atomic emission spectra (ICP-AES). A mechanism proposed herein indicates adsorption and Fenton-like reaction (heterogeneous and homogeneous) are responsible for the discoloration of BF-BR.

  16. Schorl: A novel catalyst in mineral-catalyzed Fenton-like system for dyeing wastewater discoloration

    Energy Technology Data Exchange (ETDEWEB)

    Xu Huanyan, E-mail: xhy7587@yahoo.com.cn [College of Material Science and Engineering, Harbin University of Science and Technology, Harbin, 150040 (China); Prasad, Murari [Environmental Chemistry Division, Advanced Materials and Processing Research Institute (C.S.I.R.), Hoshangabad Road, Habibganj Naka, Bhopal 462006 (India); Liu Yu [College of Zijin Mining Engineering, Fuzhou University, Fuzhou, 350108 (China)

    2009-06-15

    Mineral-catalyzed Fenton-like system has been found to be effective for the discoloration of dyeing wastewater. In our present study, schorl has been successfully developed as a novel heterogeneous catalyst for discoloration of an active commercial dye, Argazol blue (BF-BR), in an aqueous solution. Through a number of batch discoloration experiments under various conditions, it was found that the reactivity of the system increased by, respectively, increasing schorl dosage, temperature, hydrogen peroxide starting concentration and by decreasing the pH. At the condition of pH 2, T = 55 {sup o}C, [BF-BR]{sub 0} = 200 mg/L, [H{sub 2}O{sub 2}]{sub 0} = 48.5 mmol/L and schorl dosage = 10 g/L, 100% of discoloration ratio can be achieved in less than 4 min, and 72% of total organic carbon (TOC) can be removed in less than 200 min. The reaction kinetics analysis shows that the discoloration of BF-BR follows the first-order kinetics. The schorl samples after BF-BR discoloration was tested by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Scanning electron microscope (SEM) and the results rule out the possibility of formation of new solid phases during BF-BR discoloration. The content of Fe ion leaching in the solution was also measured using inductively coupling plasma-atomic emission spectra (ICP-AES). A mechanism proposed herein indicates adsorption and Fenton-like reaction (heterogeneous and homogeneous) are responsible for the discoloration of BF-BR.

  17. Improving the water use efficiency of short rotation coppice (SRC) willows

    Energy Technology Data Exchange (ETDEWEB)

    Stephens, W.; Bonneau, L.; Groves, S.; Armstrong, A.; Lindegard, K.

    2003-07-01

    On the premise that biofuels will make a significant contribution to the UK's renewable energy sources by the year 2010, willow short rotation coppicing is being studied. The high water requirement of willow is seen as a potential problem in the drier regions of the UK and increasing the water use efficiency and/or draught resistance would extend the areas where willow coppicing would be profitable. The first part of the project was to investigate the water use of a number of near-market varieties of willow and evaluate techniques for early drought tolerance screening in a breeding program and for this, field studies were conducted. This report gives some very early results from the preliminary study. Since DTI funding ceased before the one-season study of the three-year program was complete, the results should be regarded as tentative only. The next phase of the program will be funded by DEFRA and will include efforts to identify how a range of high-yielding willows respond to water stress.

  18. Comparison of Chemical and Physical-chemical Wastewater Discoloring Methods

    Directory of Open Access Journals (Sweden)

    Durašević, V.

    2007-11-01

    Full Text Available Today's chemical and physical-chemical wastewater discoloration methods do not completely meet demands regarding degree of discoloration. In this paper discoloration was performed using Fenton (FeSO4 . 7 H2O + H2O2 + H2SO4 and Fenton-like (FeCl3 . 6 H2O + H2O2 + HCOOH chemical methods and physical-chemical method of coagulation/flocculation (using poly-electrolyte (POEL combining anion active coagulant (modified poly-acrylamides and cationic flocculant (product of nitrogen compounds in combination with adsorption on activated carbon. Suitability of aforementioned methods was investigated on reactive and acid dyes, regarding their most common use in the textile industry. Also, investigations on dyes of different chromogen (anthraquinone, phthalocyanine, azo and xanthene were carried out in order to determine the importance of molecular spatial structure. Oxidative effect of Fenton and Fenton-like reagents resulted in decomposition of colored chromogen and high degree of discoloration. However, the problem is the inability of adding POEL in stechiometrical ratio (also present in physical-chemical methods, when the phenomenon of overdosing coagulants occurs in order to obtain a higher degree of discoloration, creating a potential danger of burdening water with POEL. Input and output water quality was controlled through spectrophotometric measurements and standard biological parameters. In addition, part of the investigations concerned industrial wastewaters obtained from dyeing cotton materials using reactive dye (C. I. Reactive Blue 19, a process that demands the use of vast amounts of electrolytes. Also, investigations of industrial wastewaters was labeled as a crucial step carried out in order to avoid serious misassumptions and false conclusions, which may arise if dyeing processes are only simulated in the laboratory.

  19. Prevention of Reservoir Interior Discoloration

    Energy Technology Data Exchange (ETDEWEB)

    Arnold, K.F.

    2001-04-03

    Contamination is anathema in reservoir production. Some of the contamination is a result of welding and some appears after welding but existed before. Oxygen was documented to be a major contributor to discoloration in welding. This study demonstrates that it can be controlled and that some of the informal cleaning processes contribute to contamination.

  20. Discolored Semen: What Does It Mean?

    Science.gov (United States)

    ... it mean? Should I be concerned about discolored semen? Answers from Todd B. Nippoldt, M.D. Semen is normally a whitish-gray color. It's usually ... within 30 minutes. Changes in the appearance of semen might be temporary and not a health concern. ...

  1. Bleaching of the discolored traumatized tooth

    DEFF Research Database (Denmark)

    Dahl, Jon E.; Kopperud, Siemen E.; Pallesen, Ulla

    2018-01-01

    This chapter focuses on the treatment of discolored traumatized teeth, most of them being non-vital and subsequently, endodontically treated. Tooth bleaching based upon hydrogen peroxide as the active agent, applied directly or produced in a chemical reaction from sodium perborate or carbamide pe...

  2. Salicilatos isolados de folhas e talos de Salix martiana Leyb. (Salicaceae Salicylates isolated from leaves and stems of Salix martiana Leyb. (Salicaceae

    Directory of Open Access Journals (Sweden)

    Carromberth Carioca Fernandes

    2009-01-01

    Full Text Available Salix martiana Leyb. is an endemic species from the Amazon river floodplain areas (varzeas, of the State of Amazonas. Stems and leaves were extracted with dichloromethane, methanol and hydro-alcohol and these extracts were fractionated by using conventional chromatographic techniques. The major substances isolated, salicin and trichocarposide (6-0-p-coumaroyl salicin, were determined through analyses of NMR 1D (¹H and 13C and NMR 2D (gHSQC and gHMBC. These compounds were isolated for the first time in Salix martiana Leyb. (Salicaceae. The percentage of these compounds in S. martiana is very high. The extracts were analyzed for their DPPH antioxidant capacity and the methanolic from the leaves and the hydro-alcoholic from the stems were the more active.

  3. Salicylates isolated from leaves and stems of Salix martiana Leyb. (Salicaceae); Salicilatos isolados de folhas e talos de Salix martiana Leyb. (Salicaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes, Carromberth Carioca [Universidade Federal do Acre (UFAC), Rio Branco, AC (Brazil). Centro de Ciencias Biologicas e da Natureza; Cursino, Lorena Mayara de Carvalho; Novaes, Jussival de Abreu Pinheiro; Demetrio, Camilla Avelino; Pereira Junior, Orlando Liborio; Nunez, Cecilia Veronica [Instituto de Pesquisa da Amazonia (INPA), Manaus, AM (Brazil). Coordenacao de Pesquisas em Produtos Naturais; Amaral, Ieda Leao do [Instituto de Pesquisa da Amazonia (INPA), Manaus, AM (Brazil). Coordenacao de Pesquisas em Botanica

    2009-07-01

    Salix martiana Leyb. is an endemic species from the Amazon river floodplain areas (varzeas), of the State of Amazonas. Stems and leaves were extracted with dichloromethane, methanol and hydro-alcohol and these extracts were fractionated by using conventional chromatographic techniques. The major substances isolated, salicin and trichocarposide (6-0-p-coumaroyl salicin), were determined through analyses of NMR 1D ({sup 1}H and {sup 13}C) and NMR 2D (gHSQC and gHMBC). These compounds were isolated for the first time in Salix martiana Leyb. (Salicaceae). The percentage of these compounds in S. martiana is very high. The extracts were analyzed for their DPPH antioxidant capacity and the methanolic from the leaves and the hydro-alcoholic from the stems were the more active. (author)

  4. The effect of soda immersion on nano hybrid composite resin discoloration

    Directory of Open Access Journals (Sweden)

    M. Chair Effendi

    2014-03-01

    Full Text Available Background: Composite resin is the tooth-colored restorative material which most of the people are fond of due to their aesthetic value. The composite resin discoloration may happen because of the intrinsic and extrinsic factors. Soda water is one of the beverages which can cause the composite resin discoloration. Purpose: The study was aimed to determine the effect of soda immersion on nano hybrid composite resin discoloration. Methods: The study was an experimental laboratory study using 100 shade A3 nano hybrid composite resin specimens with the diameter of 5 mm and density of 2mm. The samples were divided into 5 groups, each group was immersed in different beverages. The beverages were mineral water; lemon-flavored soda; strawberry-flavored soda; fruit punch-flavored soda; and orange-flavored soda for 3, 7, 14 and 21 days respectively, in the temperature of 37o C. The discoloration measurement utilizes Spectrophotometer, Vita Easy Shade, and uses CIEL*a*b* method. Results: The result showed that the duration of immersion in soda had an effect on the Nano hybrid composite resin discoloration. Strawberry and fruit punch- flavored soda were the most influential components toward the discoloration. Nevertheless, the generally-occurred discoloration was clinically acceptable (∆E ≤ 3,3. Conclusion: The study suggested that the soda immersion duration has effect on Nano hybrid composite resin discoloration.Latar belakang: Resin komposit adalah material sewarna gigi yang diminati masyarakat karena memiliki nilai estetik yang baik. Perubahan warna resin komposit dapat terjadi karena faktor intrinsik dan ekstrinsik. Minuman soda merupakan salah satu minuman yang dapat menyebabkan perubahan warna pada resin komposit. Tujuan: Tujuan dari penelitian ini untuk meneliti perubahan warna resin komposit nanohibrida akibat perendaman dalam minuman soda. Metode: Metode yang digunakan pada penelitian ini adalah eksperimental laboratorik dengan menggunakan

  5. Effectiveness of bleaching agent on composite resin discoloration

    Directory of Open Access Journals (Sweden)

    Galih Sampoerno

    2012-03-01

    Full Text Available Background: The discoloration of teeth, especially anterior teeth, is one of aesthetic problems. The use of tooth bleaching agents for discolored natural teeth is becoming increasingly popular. Many dentists, however, get many problems when they conduct bleaching process since there is much composite filling on patient’s anterior teeth. Although many research have focused on the discoloration of composite resin after bleaching process, the problem still becomes debatable. Purpose: The purpose of this study was to investigate the difference of the discoloration between hybrid composite and nano composite before and after the application of tooth bleaching agent, 38% hydrogen peroxide. Methods: Eighteen disk-shaped specimens (5 mm of each of two composite resins, hybrid and nano filler, were prepared. The each group was treated 3 times and the specimens were divided into two groups consisted of 9 specimens for each, and then immersed in black tea solutions for 72 hours. Next, after having staining and bleaching processes, the color of the specimens was measured with a optic spectrophotometer by using photo with type BPY-47 and digital microvolt. The differences of the light intensity among three measurements were then calculated. Afterwards, GLM MANOVA Repeated Measure and parametric analysis (Independent t-test and Paired t-test were then used to analyze the data. Results: After staining process, it is then known that the nano composite had more discoloration and more affected by the black tea solution than the hybrid one. Conclusion: After bleaching, the discoloration was finally removed completely from both hybride and nano filler composite resins and became brighter from the baseline color.Latar belakang: Salah satu problem estetik adalah adanya perubahan warna pada gigi anterior. Peningkatan pemakaian bahan bleaching semakin popular. Banyak dokter gigi mempunyai problem ketika mereka akan melakukan proses bleaching dan ditemukan banyak

  6. Coastal Energy Corporation, Willow Springs, MO

    Science.gov (United States)

    notice of a proposed Administrative Penalty Assessment against Coastal Energy Corporation, located at 232 Burnham Road, Willow Springs, Missouri, for alleged violations at the facility located at or near that facility.

  7. Moessbauer Study of Discoloration of Synthetic Resin Covered Electric Switches

    International Nuclear Information System (INIS)

    Kuzmann, E.; Muzsay, I.; Homonnay, Z.; Vertes, A.

    2002-01-01

    57 Fe Moessbauer spectroscopy and X-ray diffractometry were used to investigate brown discoloration and sediments formed on the surface of synthetic resin product covered electronic switches. The Moessbauer measurement revealed that alloyed steels and iron-containing corrosion products are associated with the discolored layers. Iron, and iron corrosion products were shown by both MS and XRD in the sediments formed eventually during the finishing of the synthetic resin products after machining and washing with water solution.

  8. Groundwater–surface water interactions, vegetation dependencies and implications for water resources management in the semi-arid Hailiutu River catchment, China – a synthesis

    Directory of Open Access Journals (Sweden)

    Y. Zhou

    2013-07-01

    Full Text Available During the last decades, large-scale land use changes took place in the Hailiutu River catchment, a semi-arid area in northwest China. These changes had significant impacts on the water resources in the area. Insights into groundwater and surface water interactions and vegetation-water dependencies help to understand these impacts and formulate sustainable water resources management policies. In this study, groundwater and surface water interactions were identified using the baseflow index at the catchment scale, and hydraulic and water temperature methods as well as event hydrograph separation techniques at the sub-catchment scale. The results show that almost 90% of the river discharge consists of groundwater. Vegetation dependencies on groundwater were analysed from the relationship between the Normalized Difference Vegetation Index (NDVI and groundwater depth at the catchment scale and along an ecohydrogeological cross-section, and by measuring the sap flow of different plants, soil water contents and groundwater levels at different research sites. The results show that all vegetation types, i.e. trees (willow (Salix matsudana and poplar (Populus simonii, bushes (salixSalix psammophila, and agricultural crops (maize – Zea mays, depend largely on groundwater as the source for transpiration. The comparative analysis indicates that maize crops use the largest amount of water, followed by poplar trees, salix bushes, and willow trees. For sustainable water use with the objective of satisfying the water demand for socio-economical development and to prevent desertification and ecological impacts on streams, more water-use-efficient crops such as sorghum, barley or millet should be promoted to reduce the consumptive water use. Willow trees should be used as wind-breaks in croplands and along roads, and drought-resistant and less water-use intensive plants (for instance native bushes should be used to vegetate sand dunes.

  9. Pelletizing using forest fuels and Salix as raw materials. A study of the pelletizing properties; Pelletering med skogsbraensle och Salix som raavara. En undersoekning av pelleterbarheten

    Energy Technology Data Exchange (ETDEWEB)

    Martinsson, Lars; Oesterberg, Stefan [Swedish National Testing and Research Inst., Boraas (Sweden)

    2004-08-01

    Three common forest fuels: light thinning material, cull tree and logging residues as well as energy forest fuel (Salix) has been used as fuel pellet materials. Logging residues and Salix were stacked for approximately 6 and 10 months respectively. Parameters varied for each raw material have been the moisture content and the press length of the die. These parameters have been changed to obtain best possible quality, mainly concerning mechanical durability. Pellets were also produced from bark free shavings in order to use as a reference in this study. Physical as well as chemical properties have been compared. It was comparatively easy to press logging residues and Salix into durable pellets and, even with larger press length, the production of pellets was higher than it was for the other raw materials. The density was equal for all pellets while the mechanical durability was better for all tested raw materials compared with the reference material. The fact that all raw materials besides the reference material contains bark which has an improving effect on the degree of hardness. The quality properties were mainly about the same or better for pellets made of light thinning material and cull tree respectively, compared with the reference pellets. However, the ash content was approximately twice as high compared with the reference pellets. The pellets made of logging residues and Salix respectively were of very good quality concerning duration and density but the ash content was approximately 10 times higher than in the reference pellets. Additionally, the nitrogen content was 6-9 times higher compared with the reference pellets.

  10. Fertilization of SRC willow. II

    DEFF Research Database (Denmark)

    Sevel, L; Ingerslev, Morten; Nord-Larsen, Thomas

    2014-01-01

    impacts of different doses of mineral fertilizer, manure and sewage sludge in a commercially grown SRC willow stand. We examined macro nutrient and heavy metal leaching rates and calculated element balances to evaluate the environmental impact. Growth responses were reported in a former paper (Sevel et al...

  11. Study on the cause of discoloration of radiation-exposed polyolefin

    Energy Technology Data Exchange (ETDEWEB)

    Jeun, Joonpyo; Kang, Philhyun; Oh, Seunghwan; Kim, Hyunbin

    2013-01-15

    Olefinic polymers, such as polyethylene and polypropylene, have a wide variety of known end use applications. Olefinic polymers have been disclosed to be useful in the manufacture of shaped articles for medical used and for food packaging uses where the articles must undergo stabilization for be disinfected. Notwithstanding the significant known advantages of sterilization by means of high energy radiation, several disadvantages are known to be associated with such sterilization techniques. Most of all, when treated with radiation energy in an amount sufficient to achieve the desired sterilization, such polyolefin compositions may become discolored. This coloration may occur for a variety of reasons such as the use of certain additives in the polymer, as well as the presence of high amounts of catalytic residues. In this study, effects of structure of antioxidant and UV stabilizer on the discoloration of olefinic polymer resin were investigated. Furthermore, The mechanism of discoloration of olefinic polymer was suggested.

  12. Degradation of Phenanthrene by a chilean white rot fungus Anthracophyllum discolor

    International Nuclear Information System (INIS)

    Acevedo, F.; Cuevas, R.; Rubilar, O.; Tortella, G.; Diez, M. C.

    2009-01-01

    Anthracophyllum discolor, a white rot fungus of southern Chile, has been an efficient degrader of clorophenols and azo dyes. This fungus produces ligninolytic enzymes being manganese peroxidase (Mn)) the major one produced. The main purpose of this study was to evaluate the effect of phenanthrene concentration of ligninolytic activity of A. Discolor measured by poly R-478 decolorazation, and to evaluate the potential of this fungus for degrading phenanthrene in liquid media. (Author)

  13. Degradation of Phenanthrene by a chilean white rot fungus Anthracophyllum discolor

    Energy Technology Data Exchange (ETDEWEB)

    Acevedo, F.; Cuevas, R.; Rubilar, O.; Tortella, G.; Diez, M. C.

    2009-07-01

    Anthracophyllum discolor, a white rot fungus of southern Chile, has been an efficient degrader of clorophenols and azo dyes. This fungus produces ligninolytic enzymes being manganese peroxidase (Mn) the major one produced. The main purpose of this study was to evaluate the effect of phenanthrene concentration of ligninolytic activity of A. Discolor measured by poly R-478 decolorazation, and to evaluate the potential of this fungus for degrading phenanthrene in liquid media. (Author)

  14. Earthworm short-term response to dairy wastewater applied to a Salix plantation

    DEFF Research Database (Denmark)

    Krogh, Paul Henning; Jørgensen, Uffe; Berg Olsen, Anne

    Odour is one problem in the management of waste water from dairy, thus the organic Thise Dairy has developed an all year round solution releasing the waste water into underground tubes at 30 cm depth in a willow energy crop plantation established in 2013. The wastewater contains 0.3% dry matter...... in the untreated control willow area, were established in Oct. 2017 and considered (non-randomized) blocks. In other words this was a Before-After-Control-Impact (BACI) design. Plots were sampled to estimate earthworm abundance one week before and one week after treatment (map link: goo.gl/tvvMuF). The willow...... was surrounded by an agricultural field with a history of wastewater fertilisation subject to an exploratory sampling. - We found seven species of earthworms in the willow and five in the agricultural soil. Juveniles of A. tuberculata, A. caliginosa, L. herculeus, L. castaneus, are morphologically intrageneric...

  15. Demonstration cultivation of Salix in northern Sweden with a focus on frost resistance; Demonstrationsodling av Salix i Norrland med frosttolerans i fokus

    Energy Technology Data Exchange (ETDEWEB)

    Soederstroem, Yvonne

    2010-06-15

    This project is part of the Thermal Engineering Research Association's (Vaermeforsk) programme 'Crops from field to energy production' and the goal is to convince growers to begin cultivating Salix in the northern part of Sweden. The project will demonstrate whether it is possible to grow Salix and examine what type of Salix (the report refers to the different types as Tora, Karin and Gudrun) is most frost-resistant and resistant to insects, fungal infections and animals. The project is aimed primarily at landowners and energy companies. The results showed that growth at the demonstration plots was moderate and the establishment rate was considered good. Growth has been uneven across the fields. At Eriksgaard, about 75% of the plants survived the winter. Survival was determined by the number of plants missing and dead. Additional plants were planted at the sites in 2009. No major insect or leaf fungus attacks were noted at either Eriksgaard or Soergaard, but they both experienced grazing from deer and elk. The rating showed that the herbicide treatment was not sufficient on the demonstration fields. For this reason, a comparison of average lengths was performed of Gudrun in Bodum, where the weeds had not grown as much. The comparison showed that the difference in growth was not significant in the autumn 2008, but the plants in Bodum had grown more by autumn 2009. Topping was not performed in the spring 2009 so that the plants would have an opportunity to grow higher than the weeds and the plants would have an opportunity to have a growth spurt. A comparison of Tora, Karin and Gudrun in Roedoen showed moderate growth for the three types. There were variations in the rows and across the field, mostly for Tora and Karin. Gudrun had lower, but more consistent growth across the field. During the summer 2009, survival was rated for the plants in Roedoen. This rating showed that 90% of Tora, 60% of Karin and 70% of Gudrun had survived the winter. The Salix

  16. Cryopreservation of Populus trichocarpa and Salix dormant buds with recovery by grafting or direct rooting.

    Science.gov (United States)

    Bonnart, Remi; Waddell, John; Haiby, Kathy; Widrlenchner, Mark P; Volk, Gayle M

    2014-01-01

    Methods are needed for the conservation of clonally maintained trees of Populus and Salix. In this work, Populus trichocarpa and Salix genetic resources were cryopreserved using dormant scions as the source explant. We quantified the recovery of cryopreserved materials that originated from diverse field environments by using either direct sprouting or grafting. Scions (either at their original moisture content of 48 to 60% or dried to 30%) were slowly cooled to -35 degree C, transferred to the vapor phase of liquid nitrogen (LNV, -160 degree C), and warmed before determining survival. Dormant buds from P. trichocarpa clones from Westport and Boardman, OR had regrowth levels between 42 and 100%. Direct rooting of cryopreserved P. trichocarpa was also possible. Ten of 11 cryopreserved Salix accessions, representing 10 different species, exhibited at least 40% bud growth and rooting after 6 weeks when a bottom-heated rooting system was implemented. We demonstrate that dormant buds of P. trichocarpa and Salix accessions can be cryopreserved and successfully regenerated without the use of tissue culture.

  17. Mycorrhizal symbioses of Salix repens : diversity and functional significance

    NARCIS (Netherlands)

    Heijden, van der E.W.

    2000-01-01

    This thesis investigates the significance of different mycorrhizal fungi, belonging to different functional types (arbuscular mycorrhiza-AM and ectomycorrhiza-EcM), in Salix repens . A comparison between above-ground and below-ground observations on ectomycorrhizal

  18. Phytopharmacology and medicinal properties of Salix aegyptiaca L.

    African Journals Online (AJOL)

    enoh

    2012-04-05

    Apr 5, 2012 ... The anti-inflammatory and antinociceptive properties of extracts of Salix family ... Iranian people for its calming effect on heart and possibly its antihypertensive .... cancer, Alzheimer's disease, Parkinson's disease and even in aging ... with patient's normal activity and are often difficult to stop once the therapy ...

  19. Uptake, metabolism, accumulation and toxicity of cyanide in willow trees

    DEFF Research Database (Denmark)

    Larsen, Morten; Ucisik, Ahmed Süheyl; Trapp, Stefan

    2005-01-01

    Chemicals taken up into plants may be accumulated so leading to toxic effects. Uptake and phytotoxicity of free cyanide was determined with the willow-tree transpiration test. Willow sets were grown in sand and irrigated with varying levels of cyanide (CN). Toxicity was determined by measuring...... transpiration. At CN concentrations below 10 mg/L, no toxic effects were observed. At 20 mg/L, transpiration was reduced to approximately 50% after 96 h. With 30, 40 and 50 mg/L, the transpiration decreased with a similar rate to cyanide in plant...... tissue was observed at 40 and 50 mg/L. The kinetics of metabolism of cyanide by roots, stems and leaves of willows was determined by the closed-bottle metabolism test. The Michaelis−Menten parameters vmax and KM (maximal metabolic velocity and half-saturation constant, respectively) were determined...

  20. Discolored Red Seaweed Pyropia yezoensis with Low Commercial Value Is a Novel Resource for Production of Agar Polysaccharides.

    Science.gov (United States)

    Sasuga, Keiji; Yamanashi, Tomoya; Nakayama, Shigeru; Ono, Syuetsu; Mikami, Koji

    2018-04-26

    The red seaweed Pyropia yezoensis has been demonstrated to be a novel resource for the production of high-quality agar. P. yezoensis is grown for the food industry in large-scale Japanese mariculture operations. However, discolored P. yezoensis is mostly discarded as an industrial waste, although it has some kind of utility values. Here, we evaluated the utility of discolored P. yezoensis as a resource for agar production. The quality of agar from the discolored seaweed was comparable to that from normal seaweed. In addition, as a distinguishing characteristic, agar yield was higher from discolored seaweeds than from normal types. Moreover, we successfully used agar from discolored P. yezoensis for bacterial plate media and DNA electrophoresis gels without agarose purification. Thus, our results demonstrate that discolored P. yezoensis is suitable for agar production and use in life science research. Diverting discolored P. yezoensis from disposal to agar production provides a solution to the current industrial waste problem in mariculture, as well as a secure source of agar for research purposes.

  1. Nutrient enhanced short rotation coppice for biomass in central Wales

    Energy Technology Data Exchange (ETDEWEB)

    Hodson, R.W.; Slater, F.M.; Lynn, S.F.; Randerson, P.F. [Univ. of Wales (United Kingdom)

    1993-12-31

    Two projects involving short rotation willow coppice are taking place on the eastern side of the Cambrian Mountains in central Wales. One project examines, as an alternative land use, the potential of short rotation willow coppice variously enhanced by combinations of lime, phosphorous and potassium fertilizers and also digested sewage sludge on an acidic upland site at an altitude of 260m. The first year results of this project are described in detail, showing the necessity for limestone additions and also demonstrating that of the four willow varieties established, Salix dasyclados is the only possible, profitable fuel crop. The other project involving willow in a filter bed system is outlined along with an additional project investigating the effect of sewage sludge additions on the Rubus fruticosus production in a birch dominated mixed deciduous woodland.

  2. Seasonal abundance and development of the Asian longhorned beetle and natural enemy prevalence in different forest types in China

    Science.gov (United States)

    Houping Liu; Leah S. Bauer; Tonghai Zhao; Ruitong Gao; Therese M. Poland

    2016-01-01

    Seasonal abundance and population development of the Asian longhorned beetle (ALB), Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae), and prevalence of its natural enemies were studied on Hankow willow (Salix matsudana Koidz.) at an urban forest site (Anci) and a rural forest site (Tangerli) in Hebei province...

  3. Application of Spent Li-Ion Batteries Cathode in Methylene Blue Dye Discoloration

    Directory of Open Access Journals (Sweden)

    Eric M. Garcia

    2017-01-01

    Full Text Available This paper aims to present the mechanism study of methylene blue (MB discoloration using spent Li-ion battery cathode tape and hydrogen peroxide. The recycled cathode used in this work is composed of 72% of LiCoO2, 18% of carbon, and 10% of Al. The value found for surface area is 8.9 m2/g and the ZCP value occurs in pH = 2.95. Different from what is proposed in the literature, the most likely mechanism of methylene blue discoloration is the oxidation/delitiation of LiCoO2 and the reduction of H2O2 forming OH∙. Thus, in this paper, an important and promising alternative for discoloration of textile industry dyes using spent Li-ion battery cathode is presented.

  4. Wound-induced proteinase inhibitor in Salix viminalis and its association with defence against insects

    Energy Technology Data Exchange (ETDEWEB)

    Saarikoski, P. [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Genetics

    1997-09-01

    For successful traditional breeding, the plant material has to be screened for genetic variation for the desired traits. By screening Salix clones for wound-induced proteinase inhibitor (PI) activity and ethylene evolution, it was possible to identify variation for both characters among the Salix clones tested. However, no correlation was observed with insect and pathogen resistance. Since there was no simple relationship between wound-induced ethylene production, accumulation of PI and pest resistance, a more systematic investigation of Salix PIs was begun. A gene (swin1.1) encoding a 21 kDa trypsin inhibitor with characteristics of Kunitz-type of PI was sequenced. The trypsin inhibitor encoded by the isolated swin1.1 gene was shown to be functional in vitro and exhibit specificity for trypsin. It is therefore likely that this PI is involved in the plant defence in Salix, since many insects have trypsin as their major digestive protease. In further support of this view, in bio-tests with poplar the mortality of the first instar larvae (Lymantria dispar) was significantly increased, both after application of the trypsin inhibitor encoded by swin1.1 directly on poplar leaves and after feeding the larvae with transgenic poplar over-expressing the swin1.1 gene. In Salix, the swin1.1 gene was shown to be induced by mechanical wounding, insect feeding and by treatment with the signalling substances salicylic and jasmonic acid. The locally wound-induced response (mechanical and insect) was greater than the systemic response. Other swin1 gene family members were also differentially expressed after the inductive treatment. 187 refs., 3 figs., 2 tabs.

  5. Willow trees from heavy metals phytoextraction as energy crops

    International Nuclear Information System (INIS)

    Šyc, Michal; Pohořelý, Michael; Kameníková, Petra; Habart, Jan; Svoboda, Karel; Punčochář, Miroslav

    2012-01-01

    Phytoextraction ability of some fast growing plant species leads to the idea of connecting biomass production with soil remediation of contaminated industrial zones and regions. This biomass will contain significant amount of heavy metals and its energetic utilization has to be considered carefully to minimize negative environmental impacts. This study was focused on potential disposal methods of willow trees contaminated by heavy metals (Cd, Cu, Pb, Zn) with the emphasis on energetic utilization of biomass. Composting seems to be suitable pre-treatment method resulting in decrease of heavy metals leachability and biomass weight reduction. The possibility of willow trees application for energetic purposes was investigated and consequently incineration tests of willow trees samples in fluidized bed reactor were realized. Distribution of selected heavy metals in different ash fractions and treatment methods of produced ashes were studied as well. -- Highlights: ► Composting is an appropriate pre-treatment method for phytoextraction crops. ► Fluidized bed combustion is suitable disposal method of phytoextraction crops. ► Ashes from phytoextraction crops combustion cannot be used as fertilizers.

  6. Kinetics and optimization on discoloration of dyeing wastewater by schorl-catalyzed fenton-like reaction

    Directory of Open Access Journals (Sweden)

    Xu Huan-Yan

    2014-01-01

    Full Text Available Kinetics and optimization on the discoloration of an active commercial dye, Argazol Blue BFBR (ABB by heterogeneous Fenton-like reaction catalyzed by natural schorl were investigated in this study. Kinetic investigations revealed that the first-order kinetic model was more favorable to describe the discoloration of ABB at different reaction conditions than the second-order and Behnajady-Modirshahla-Ghanbery models. The relationship between the reaction rate constant k and reaction temperature T followed the Arrhenius equation, with the apparent activation energy Ea of 51.31kJ•mol-1. The central composite design under the response surface methodology was employed for the experimental design and optimization of the ABB discoloration process. The significance of a second order polynomial model for predicting the optimal values of ABB discoloration was evaluated by the analysis of variance and 3D response surface plots for the interactions between two variables were constructed. Then, the optimum conditions were determined.

  7. Erratum to: Fertilization of SRC Willow

    DEFF Research Database (Denmark)

    Sevel, L; Ingerslev, Morten; Nord-Larsen, Thomas

    2014-01-01

    impacts of different doses of mineral fertilizer, manure and sewage sludge in a commercially grown SRC willow stand. We examined macro nutrient and heavy metal leaching rates and calculated element balances to evaluate the environmental impact. Growth responses were reported in a former paper (Sevel et al...

  8. Willow Browse Survey 2016 : Biological Summary Report

    Data.gov (United States)

    Department of the Interior — Browse pressure by ungulate species, primarily elk, is known to have a negative impact on the health of willow stands. On Baca and Alamosa NWR’s, it has been noticed...

  9. Willow Browse Survey 2015 : Biological Summary Report

    Data.gov (United States)

    Department of the Interior — Browse pressure by ungulate species, primarily elk, is known to have a negative impact on the health of willow stands. On Baca and Alamosa NWR’s, it has been noticed...

  10. Willow as fuel for district heating. Experiences from test combustion; Energipil som braendsel til fjernvarme - Erfaringsindsamling fra testfyringer

    Energy Technology Data Exchange (ETDEWEB)

    Pedersen, Joergen

    2012-10-15

    The project has been a study of the fuel characteristics of willow chips. The study was carried out on Trustrup-Lyngby Heating Plant and Assens District Heating Plant in the period 2011-12. Operating experiences were collected from the two plants. Furthermore, yield and crop data were collected from suppliers of willow chips to Assens District Heating Plant, and the analysis of particle size distribution of the willow chips is carried out. The collected data on yield and particle size distribution are compared with results from previous studies. The project has shown that willow chips generally are a suitable and attractive fuel in wood-fired heat and power plants. The plants are very aware of quality of willow chips and want chips with coarse structure. Furthermore, there is the wish that water content of willow chips are on par with the moisture content of wood chips, i.e. around 30-40%; woodchips are the vast majority of the chips used in the plants. Wood chips produced from fresh willow shoots with chopper will typically have a moisture content of 50-60 %. Such ''wet'' chips will of some plants be deselected during winter, where there is a requirement of safe and high boiler output. Other plants will simply mix the ''wet'' willow chips with other, drier types of chips and can use it almost all the year. If the willow shoots are harvested as branches, which subsequently are allowed to dry for a period before chipping, willow chips can be produced with a moisture content that is in line with what is typical in wood chips. Analysis of particle size distribution shows that willow chips harvested with a cutting machine usually can meet the requirements for quality classes ''fine'', ''medium'' and ''coarse''. An account of the harvested yields of willow among the growers who supplied willow chips to Assens Heating Plant, showed a relatively low yield of 5.1 tonnes dry

  11. Cultural Resources Investigations in the Vicinity of Fort Jackson, Plaquemines Parish, Louisiana: The Proposed Solari Borrow Area

    Science.gov (United States)

    1989-08-01

    tory of flora. Trees such as Virginia live oak (Quercus virginiana), black willow (Salix nigra), hackberry (Celtislaevi- gata), and persimmon ( Diospyros ...bullfrog, is a human food source today and may have been prehistorically as well. The toad, however, of the genus Bufo is not normally consumed

  12. Effects of long-term Salix cultivation on total and plant-available contents of Cadmium in the soil - a pilot study

    International Nuclear Information System (INIS)

    Eriksson, Jan; Ledin, S.

    1996-02-01

    The aim of the project was to study how total and plant-available contents of Cadmium in the soil are affected by the large amounts of Cadmium that are removed during the harvesting of Salix. Eight long-term Salix plantations, 8-30 years old, were chosen. At each place soil samples were taken in 4 areas in the stands of Salix and in 4 neighbouring areas with comparable soil conditions, but without Salix (reference areas). Cd in three fractions of different bonding strength were determined in the soil samples. The fractions roughly correspond to the total concentration, the exchangeable fraction, and the fraction dissolved in the soil solution. The result showed a relatively minor effect of the Salix plantation on the total concentrations. In six of eight cases, however, the concentrations tended to be lower in the Salix plantations than in the reference areas. When consideration was given to certain pH differences, the exchangeable, and particularly the most soluble fraction, showed a clear tendency for concentrations to be lower in the Salix stands than in the reference areas.The concentrations in stem samples from growing stands were generally lower than those measured in harvest-mature stems in other studies. The concentrations in foliage were of the same magnitude as those in the stems, implying that there is an important return of Cd to the soil at leaf-fall. The negligible effect on the total content in the soil may depend on uptake occurring both in the topsoil and in the subsoil. Re-circulation via the leaves will also result in redistribution of Cd from the subsoil to the topsoil, compensating the uptake from the topsoil. The conclusion reached was that Salix cultivation results in a reduction of the plant-available Cd in the soil, but the effect is not concentrated to the topsoil. 13 refs, 5 tabs, 4 figs

  13. Nanoscale Phenomena Occurring during Pyrolysis of Salix viminalis Wood

    Directory of Open Access Journals (Sweden)

    Aleksandra W. Cyganiuk

    2013-01-01

    Full Text Available Selective utilisation of unique properties of Salix viminalis wood enables preparation of materials of nanotechnologic properties. Thermal decomposition of lignin-cellulose organic matter results in the formation of a nanostructured porous carbon matrix (charcoal. Narrowed pore size distribution (PSD in the subnanometer range allows to consider the charcoals as carbon molecular sieves (CMSs, which are capable of separating even chemically inert gases like neon, krypton, and nitrogen. High tolerance of Salix viminalis to heavy metal ions enables enriching living plant tissues with metal ions like lanthanum and manganese. Such ions may later form LaMnO3 with parallel transformation of plant tissues (organic matter to carbon matrix using a heat treatment. In this way, one gets a hybrid material: a porous carbon matrix with uniformly suspended nanocrystallites of LaMoO3. The crystallites are in the catalytically active phase during the conversion of n-butanol to heptanone-4 with high yield and selectivity.

  14. Puente Willow Creek en Monterrey, California

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1965-09-01

    Full Text Available Of the 10 awards given every year by the Prestressed Concrete Institute for the most outstanding prestressed concrete projects, two have been awarded in California this year, one of them to the Willow Creek bridge, near Monterrey. The prestressed, double T girders of this bridge were made at a workshop, a great distance from the bridge site. These are 24 m long, 1.35 m high, and are stabilized by transversal diaphragms, 20 cm in thickness. The table deck is of reinforced concrete, being 8.85 m wide and 20 cm thick. The structure is straightforward, slender, and adapts itself pleasantly to the background. It has seven spans and crosses over a secondary road, in addition to bridging the Willow stream. The supporting piles are hollow, of rectangular cross section, and over them a cross beam carries the five girders and the deck itself. The end abutments consist of vertical reinforced concrete walls, and supporting, soil filled, structures. The above information was supplied by the California Road Department.De los diez premios que anualmente concede el Prestressed Concrete Institute para las obras de hormigón pretensado más notables, dos han correspondido a California y uno de ellos al puente de Willow Creek, situado en la región de Monterrey. Las vigas de hormigón pretensado, con sección en forma de doble T, se prefabricaron en un taller situado a gran distancia del puente. Tienen 24 m de longitud y 1,35 m de canto, estando arriostradas con diafragmas transversales de 20 cm de espesor. La losa del tablero, de hormigón armado, tiene 8,85 m de anchura y 20 cm de espesor. La estructura es sencilla, esbelta y armoniza perfectamente con el paisaje que la circunda. Tiene siete tramos y salva un paso inferior secundario y el arroyo Willow. Los soportes, se apoyan sobre pilotes, algunos de gran altura; son huecos, de sección rectangular y terminan en una cruceta que sirve de sostén a las cinco vigas que soportan la losa del tablero. Los estribos

  15. Evaluation of Populus and Salix continuously irrigated with landfill leachate I. Genotype-specific elemental phytoremediation.

    Science.gov (United States)

    Zalesny, Ronald S; Bauer, Edmund O

    2007-01-01

    There is a need for the identification and selection of specific tree genotypes that can sequester elements from contaminated soils, with elevated rates of uptake. We irrigated Populus (DN17, DN182, DN34, NM2, NM6) and Salix (94003, 94012, S287, S566, SX61) genotypes planted in large soil-filled containers with landfill leachate or municipal water and tested for differences in inorganic element concentrations (P, K, Ca, Mg, S, Zn, B, Mn, Fe, Cu, Al, Na, and Cl) in the leaves, stems, and roots. Trees were irrigated with leachate or water during the final 12 wk of the 18-wk study. Genotype-specific uptake existed. For genera, tissue concentrations exhibited four responses. First, Populus had the greatest uptake of P, K, S, Cu, and Cl. Second, Salix exhibited the greatest uptake of Zn, B, Fe, and Al. Third, Salix had greater concentrations of Ca and Mg in leaves, while Populus had greater concentrations in stems and roots. Fourth, Populus had greater concentrations of Mn and Na in leaves and stems, while Salix had greater concentrations in roots. Populus deltoides x P. nigra clones exhibited better overall phytoremediation than the P. nigra x P. maximowiczii genotypes tested. Phytoremediation for S. purpurea clones 94003 and 94012 was generally less than for other Salix genotypes. Overall, concentrations of elements in the leaves, stems, and roots corroborated those in the plant-sciences literature. Uptake was dependent upon the specific genotype for most elements. Our results corroborated the need for further testing and selecting of specific clones for various phytoremediation needs, while providing a baseline for future researchers developing additional studies and resource managers conducting on-site remediation.

  16. Aufnahme von Cyanid in Pflanzen

    DEFF Research Database (Denmark)

    Trapp, Stefan; Koch, Ines; Christiansen, Helle

    2001-01-01

    Cyanides are waste products from the pyrolysis of coal and are frequent soil pollutants in cities nowadays. Prussic acid (HCN) is a fast acting, highly toxic poison, but iron-complexed cyanides in soil are far less toxic. The phytotoxicity of free CN to basket willows (Salix viminalis) was determ...

  17. 7 CFR 301.51-2 - Regulated articles.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Regulated articles. 301.51-2 Section 301.51-2... Regulated articles. The following are regulated articles: (a) Firewood (all hardwood species), and green... (sycamore), Populus (poplar), Salix (willow), Sorbus (mountain ash), and Ulmus (elm). (b) Any other article...

  18. Machinery management data for willow harvest with a bio-baler

    Energy Technology Data Exchange (ETDEWEB)

    Savoie, P. [Agriculture and Agri-Food Canada, Quebec City, PQ (Canada); Hebert, P.L. [Laval Univ., Quebec City, PQ (Canada). Dept. des sols et de genie agroalimentaire; Robert, F.S. [Laval Univ., Quebec City, PQ (Canada). Sols et environnement

    2010-07-01

    Willow harvested in 2-to-3-year rotations has been touted as a reliable source of biomass. A newly designed harvester based on a round baler was developed in 2006. The third generation biobaler was evaluated in 2009 at 2 willow plantations in Quebec. The first site at Godmanchester was a three-year old regrowth with an estimated 52,000 stems/ha of large diameter willow. The biobaler with a flail cutter harvested three plots totalling 4,136 m{sup 2}. Total harvested biomass was 10.36 t DM in 42 bales. The harvest rate averaged 29 bale/h with an average bale size of 1.22 m wide by 1.30 m in diameter. Diesel fuel consumption averaged 0.81 L/bale. Measured losses averaged 11 per cent of the yield. The second site at Saint-Roch-del'Achigan was a two-year old growth with an estimated 49,000 stems/ha of large stem willow. The biobaler harvested seven plots totalling 15,740 m{sup 2}. Total harvest was 30.70 t DM in 148 bales. The first 14 bales were harvested with a saw blade header. All other bales were harvested with the flail cutter at a faster rate. Bale density averaged 129 kg DM/m{sup 3}. Fuel consumption averaged 0.72 L/bale. Losses averaged 20 per cent of the yield with the saw blade header.

  19. Life cycle assessment of a willow bioenergy cropping system

    International Nuclear Information System (INIS)

    Heller, M.C.; Keoleian, G.A.; Volk, Timothy A.

    2003-01-01

    The environmental performance of willow biomass crop production systems in New York (NY) is analyzed using life cycle assessment (LCA) methodology. The base-case, which represents current practices in NY, produces 55 units of biomass energy per unit of fossil energy consumed over the biomass crop's 23-year lifetime. Inorganic nitrogen fertilizer inputs have a strong influence on overall system performance, accounting for 37% of the non-renewable fossil energy input into the system. Net energy ratio varies from 58 to below 40 as a function of fertilizer application rate, but application rate also has implications on the system nutrient balance. Substituting inorganic N fertilizer with sewage sludge biosolids increases the net energy ratio of the willow biomass crop production system by more than 40%. While CO 2 emitted in combusting dedicated biomass is balanced by CO 2 adsorbed in the growing biomass, production processes contribute to the system's net global warming potential. Taking into account direct and indirect fuel use, N 2 O emissions from applied fertilizer and leaf litter, and carbon sequestration in below ground biomass and soil carbon, the net greenhouse gas emissions total 0.68 g CO 2 eq. MJ biomassproduced -1 . Site specific parameters such as soil carbon sequestration could easily offset these emissions resulting in a net reduction of greenhouse gases. Assuming reasonable biomass transportation distance and energy conversion efficiencies, this study implies that generating electricity from willow biomass crops could produce 11 units of electricity per unit of fossil energy consumed. Results form the LCA support the assertion that willow biomass crops are sustainable from an energy balance perspective and contribute additional environmental benefits

  20. Phytoremediation, a sustainable remediation technology? Conclusions from a case study. I: Energy production and carbon dioxide abatement

    International Nuclear Information System (INIS)

    Witters, N.; Mendelsohn, R.O.; Van Slycken, S.; Weyens, N.; Schreurs, E.; Meers, E.; Tack, F.; Carleer, R.; Vangronsveld, J.

    2012-01-01

    This study examines the renewable energy production of crops used for phytoremediation. Our analysis is based on a case study in the Campine region (Belgium and The Netherlands), where agricultural soils are diffusely contaminated with cadmium, lead, and zinc, with an enhanced risk for uptake of these metals in crops and leaching to the groundwater. However, the area has such a large extent (700 km 2 ) that conventional remediation is not applicable. Cultivation of crops for energy purposes on such land offers the opportunity to come up with an approach that efficiently uses contaminated agricultural land and that can be beneficial for both farmer and society. Performing a Life Cycle Analysis (LCA), we examined the energy and CO 2 abatement potential of willow (Salix spp.), silage maize (Zea mays L.), and rapeseed (Brassica napus L.) originating from contaminated land. Taking into account the marginal impact of the metals in the biomass on the energy conversion efficiency and on the potential use of the biomass and its rest products after conversion, digestion of silage maize with combustion of the contaminated digestate shows the best energetic and CO 2 abating perspectives. The replacement of cokes based electricity by willow is more efficient in CO 2 abatement than willow used in a Combined Heat and Power (CHP) unit, despite lower net energy production in the former option. Willow reaches the same energy production and same CO 2 abatement per hectare per year as silage maize when its biomass yield is respectively 13 and 8.7 Mg dm ha −1 y −1 . -- Highlights: ► We study the energy potential of Salix, Zea mays and Brassica after phytoremediation. ► The case study contains agricultural soils that are contaminated with cadmium. ► We include the impact of metals on energy conversion efficiency and rest product use. ► Higher biomass yields for Salix would make it energetically competitive with Z. mays.

  1. Mortality of radio collared willow ptarmigan in Smoela wind-power plant

    Energy Technology Data Exchange (ETDEWEB)

    Pedersen, Hans Chr.; Broeseth, Henrik; Nilsen, Erlend B.; Sandercock, Brett K.; Bevanger, Kjetil

    2011-07-01

    Full text: In the project Pre- and post-construction studies of conflicts between birds and wind turbines in coastal Norway (BirdWind), Smoela willow ptarmigan (Lagopus lagopus variegatus) has been one of the species studied. This sub-species represent gallinaceous birds, known to be bad flyers susceptible to collide with artificial structures, e.g. power lines. However, the possible impact of a wind-power plant on survival and behaviour in a willow ptarmigan population was unknown. The objectives of the study were therefore rather wide; to study direct and indirect effects of wind turbines on willow ptarmigan behaviour, habitat selection, reproduction and survival in areas where wind-power plants are established. To collect data on habitat selection, movements, collision risks, avoidance behaviour, survival and general population dynamic parameters, willow ptarmigan were radio-tagged in 2008-2010, using traditional VHF-transmitters (Holohill) with mortality switch, necklace mount, 12 g, lasting for approximately 24 months. Due to low population density and only occasional snow cover, a method using strong lights, dipnet and car was used to catch birds. In total, 34 willow ptarmigan were caught (19 males and 15 females). All birds were caught inside the wind-power plant area (WPA). The birds were radio-tracked at irregular intervals and almost all birds, when found, was located within the WPA. All carcasses of dead birds were examined. When possible, cause of death was determined as; predation (raptor), collision, unknown, other. In total, 28 of the radio-tagged birds have died since January 2008. A Kaplan-Meier analysis of cumulative survival rates, show an exceptionally low survival (<30%). Unlike other willow ptarmigan populations most of the mortality takes place during winter, from December throughout March. Although a thorough analysis of mortality causes has yet not been carried out, a majority of the birds have probably been killed by avian predators and to

  2. Fungal Distribution and Varieties Resistance to Kernel Discoloration in Korean Two-rowed Barley

    OpenAIRE

    Sang-Hyun Shin; Eun-Jo Seo; Jae-Seong Choi; JungKwan Lee; Jong-Chul Park; Chun-Sik Kang

    2013-01-01

    Barley kernel discoloration (KD) leads to substantial loss in value through downgrading and discounting of malting barley. The objective of this research is to investigate fungal distribution and varieties resistance to KD in Korean two-rowed barley. Several fungal organisms including Alternaria spp., Fusarium spp., Aspergillus spp., Epicoccum spp. and Rhizopus spp. were isolated from Korean two-rowed barley representing KD. The symptoms of KD were brown and black discolorations o...

  3. Characterization of the complete mitochondrial genomes of two whipworms Trichuris ovis and Trichuris discolor (Nematoda: Trichuridae).

    Science.gov (United States)

    Liu, Guo-Hua; Wang, Yan; Xu, Min-Jun; Zhou, Dong-Hui; Ye, Yong-Gang; Li, Jia-Yuan; Song, Hui-Qun; Lin, Rui-Qing; Zhu, Xing-Quan

    2012-12-01

    For many years, whipworms (Trichuris spp.) have been described with a relatively narrow range of both morphological and biometrical features. Moreover, there has been insufficient discrimination between congeners (or closely related species). In the present study, we determined the complete mitochondrial (mt) genomes of two whipworms Trichuris ovis and Trichuris discolor, compared them and then tested the hypothesis that T. ovis and T. discolor are distinct species by phylogenetic analyses using Bayesian inference, maximum likelihood and maximum parsimony) based on the deduced amino acid sequences of the mt protein-coding genes. The complete mt genomes of T. ovis and T. discolor were 13,946 bp and 13,904 bp in size, respectively. Both mt genomes are circular, and consist of 37 genes, including 13 genes coding for proteins, 2 genes for rRNA, and 22 genes for tRNA. The gene content and arrangement are identical to that of human and pig whipworms Trichuris trichiura and Trichuris suis. Taken together, these analyses showed genetic distinctiveness and strongly supported the recent proposal that T. ovis and T. discolor are distinct species using nuclear ribosomal DNA and a portion of the mtDNA sequence dataset. The availability of the complete mtDNA sequences of T. ovis and T. discolor provides novel genetic markers for studying the population genetics, diagnostics and molecular epidemiology of T. ovis and T. discolor. Copyright © 2012 Elsevier B.V. All rights reserved.

  4. Relationships between rare plants of the White Mountains and the late Cenozoic geology of the Colorado Plateau

    Science.gov (United States)

    Jonathan W. Long

    2007-01-01

    A complex geologic history has shaped the distribution of Arizona willow (Salix arizonica Dorn) and the Mogollon paintbrush (Castilleja mogollonica Pennell). These subalpine plants do not appear to be strict substrate specialists, but they do seem to favor coarse-textured and well-watered soils. Most of their occupied habitats were...

  5. Cultural Resources Survey of Mile 306.3 to 293.4-R on the Mississippi River, Concordia, Pointe Coupee and West Feliciana Parishes, Louisiana

    Science.gov (United States)

    1993-01-01

    willow (Salix nigra) and pecan (Carya illinoensis ), among others, were abundant and well rooted. The soils in these segments of the project area...or older. Two of the molars have small pit caries . The fourth molar may be from a younger individual, perhaps around 16 years of age. This tooth shows

  6. Biomass power for rural development. Technical progress report Phase-II. Contractual reporting period October-December 1999

    Energy Technology Data Exchange (ETDEWEB)

    Neuhauser, Edward; The Salix Consortium

    2000-03-23

    The project undertaken by the Salix Consortium is a multi-phased, multi-partner endeavor. Phase 1 focused on initial development and testing of the technology and forging the necessary agreements to demonstrate commercial willow production. The Phase 1 objectives have been successfully completed: preparing design plans for 2 utility pulverized coal boilers for 20 MW of biopower capacity; developing fuel supply plans for the project with a goal of establishing 365 ha (900 ac) of willow; obtaining power production commitments from the power companies for Phase 2; obtaining construction and environmental permits; and developing an experimental strategy for crop production and power generation improvements needed to assure commercial success. The R and D effort also addresses environmental issues pertaining to introduction of the willow energy system.

  7. Choices of action and its influence on farmers' attitudes regarding willow and reed canary grass cultivation; Betydelsen av olika handlingsalternativ foer oekat intresse hos lantbrukare att odla salix och roerflen

    Energy Technology Data Exchange (ETDEWEB)

    Jonsson, Aasa; Paulrud, Susanne; Laitila, Thomas

    2010-02-15

    The contribution of energy crops from the agricultural sector is an example of a resource that has a potential to increase substantially in the near future. However, one problem is the lack of interest in cultivating energy crops within the agricultural Several studies have shown that there are a number of reasons for farmers not becoming involved in energy crops. Besides biological aspects, market potential and profitability the farmers are also affected by the available resources on the farm, the effect of the crops on the surroundings, work load, liquidity and the perceived risks. The overall aim with the present study is to increase the knowledge about how to change farmers' attitudes regarding energy crop cultivation, i.e. making them more interested. This study also shows that there is a large fraction of farmers that generally believe they have no or little knowledge of energy crops, such as willow, reed canary grass and hemp. The fraction of farmers considering there is a larger probability of future cultivation of these crops with increased knowledge is also quite large. Energy crop cultivation does not suit everybody. Generally, growing willow and reed canary grass is an alternative for farmers having another business besides agricultural business. Characteristics for these farms are working hours <1500 hours and that the machines are partly owned

  8. Stock characterization and improvement: DNA fingerprinting and cold tolerance of Populus and Salix clones

    Energy Technology Data Exchange (ETDEWEB)

    Lin, Dolly; Hubbes, M.; Zsuffa, L. [Toronto Univ., ON (Canada). Faculty of Forestry; Tsarouhas, V.; Gullberg, U. [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Genetics; Howe, G.; Hackett, W.; Gardner, G.; Furnier, G. [Minnesota Univ., St. Paul, MN (United States). Dept. of Forest Resources; Tuskan, G. [Oak Ridge National Lab., TN (United States)

    1998-12-31

    Molecular characterization of advanced-generation pedigrees and evaluation of cold tolerance are two aspects of Populus and Salix genetic improvement programmes worldwide that have traditionally received little emphasis. As such, chloroplast DNA markers were tested as a means of determining multi-generation parental contributions to hybrid progeny. Likewise, greenhouse, growth chamber and field studies were used to assess cold tolerance in Populus and Salix. Chloroplast DNA markers did not reveal size polymorphisms among four tested Populus species, but did produce sequence polymorphisms between P. maximowiczii and P. trichocarpa. Additional crosses between multiple genotypes from each species are being used to evaluate the utility of the detected polymorphism for ascertaining parentage within interspecific crosses. Short-day, cold tolerance greenhouse studies revealed that bud set date and frost damage are moderately heritable and genetically correlated in Populus. The relationship between greenhouse and field studies suggests that factors other than short days contribute to cold tolerance in Populus. In Salix, response to artificial fall conditioning varied among full-sibling families, with the fastest growing family displaying the greatest amount of cold tolerance 26 refs, 3 tabs

  9. Birefringent Crystals and Abdominal Discoloration in the Predatory Mite Phytoseiulus persimilis (Acari: Phytoseiidae)

    Science.gov (United States)

    Bjørnson; Steiner; Keddie

    1997-03-01

    In response to grower complaints of poor performance of Phytoseiulus persimilis, mites from 14 commercial insectaries and research colonies were examined for pathogens. Some were found to have abdominal discolorations, manifested initially as two white stripes along the dorsal sides of the body within the Malpighian tubules. Advanced signs appeared as a large, centrally located, white spot or U-shaped discoloration in the distal opisthosoma within the rectum/anal atrium. White material often accumulated and hardened within the anus and formed a rectal plug that inhibited further excretion. Most affected mites were lethargic. Adults and immatures with abdominal discoloration contained numerous densely packed, birefringent, dumbbell-shaped entities. Though occasionally observed in the colon, they occurred most frequently within the Malpighian tubules and/or rectum and anal atrium. Dumbbells measured 2-4 &mgr;m long and contained prominent concentric rings. When observed by transmission electron microscopy, the entities lacked cellular organelles. Asymptomatic mites contained few or no such entities. Dumbbell-shaped inclusions were observed in P. persimilis from all sources examined. High levels of potassium, low levels of phosphorous and sulfur, and traces of chlorine were detected by energy-dispersive X-ray analysis. Guanine and uric acid, known nitrogenous wastes of arachnids, do not contain these elements. The chemical composition and structure indicate that the dumbbells are crystals. Both asymptomatic mites and those specimens exhibiting abdominal discoloration were examined for pathogens using light and transmission electron microscopy. Microsporidia, virus-like particles, and a rickettsia (genus Wolbachia) were observed in some mites but showed no correlation with white abdominal discoloration or associated crystal formation. Neither were pathogens always detected in symptomatic mites. Although birefringent crystals may be naturally occurring excretory

  10. Growdon Gate/Road Relocation and Property Acquisition Environmental Assessment. Volume 1

    Science.gov (United States)

    2012-11-01

    black walnut (Juglans nigra), pecan ( Carya illinoensis ), blackberry (Rubus sp.), greenbriar (Smilax sp.), poison ivy (Rhus radicans), giant...Riparian Cedar elm (Ulmus crassifolia), black willow (Salix nigra), hackberry (Celtis laevigata), chinaberry (Melia azedarach), pecan ( Carya ... illinoensis ), Canada wildrye (Elymus candensis), poison ivy (Rhus radicans), greenbrier (Smilax spp.), and giant ragweed (Ambrosia trifida) Urban

  11. Foraging intention affects whether willow tits call to attract members of mixed-species flocks.

    Science.gov (United States)

    Suzuki, Toshitaka N; Kutsukake, Nobuyuki

    2017-06-01

    Understanding how individual behaviour influences the spatial and temporal distribution of other species is necessary to resolve the complex structure of species assemblages. Mixed-species bird flocks provide an ideal opportunity to investigate this issue, because members of the flocks are involved in a variety of behavioural interactions between species. Willow tits ( Poecile montanus ) often produce loud calls when visiting a new foraging patch to recruit other members of mixed-species flocks. The costs and benefits of flocking would differ with individual foraging behaviours (i.e. immediate consumption or caching); thus, willow tits may adjust the production of loud calls according to their foraging intention. In this study, we investigated the link between foraging decisions and calling behaviour in willow tits and tested its influence on the temporal cohesion with members of mixed-species flocks. Observations at experimental foraging patches showed that willow tits produced more calls when they consumed food items compared with when they cached them. Playback experiments revealed that these calls attracted flock members and helped to maintain their presence at foraging patches. Thus, willow tits adjusted calling behaviour according to their foraging intention, thereby coordinating the associations with members of mixed-species flocks. Our findings demonstrate the influence of individual decision-making on temporal cohesion with other species and highlight the importance of interspecific communication in mixed-species flocking dynamics.

  12. Evaluation of the Efficacy of Crude Extracts of Salix subserrata and ...

    African Journals Online (AJOL)

    aqueous of Salix subserrata were found to increase the survival time of mice signifi- cantly (P< ... identification and authentication was done using the manual of the Depart- ... Data were analyzed using SPSS version 11.5 for windows software.

  13. Characterizing the discoloration of methylene blue in Fe0/H2O systems.

    Science.gov (United States)

    Noubactep, C

    2009-07-15

    Methylene blue (MB) was used as a model molecule to characterize the aqueous reactivity of metallic iron in Fe(0)/H(2)O systems. Likely discoloration mechanisms under used experimental conditions are: (i) adsorption onto Fe(0) and Fe(0) corrosion products (CP), (ii) co-precipitation with in situ generated iron CP, (iii) reduction to colorless leukomethylene blue (LMB). MB mineralization (oxidation to CO(2)) is not expected. The kinetics of MB discoloration by Fe(0), Fe(2)O(3), Fe(3)O(4), MnO(2), and granular activated carbon were investigated in assay tubes under mechanically non-disturbed conditions. The evolution of MB discoloration was monitored spectrophotometrically. The effect of availability of CP, Fe(0) source, shaking rate, initial pH value, and chemical properties of the solution were studied. The results present evidence supporting co-precipitation of MB with in situ generated iron CP as main discoloration mechanism. Under high shaking intensities (>150 min(-1)), increased CP generation yields a brownish solution which disturbed MB determination, showing that a too high shear stress induced the suspension of in situ generated corrosion products. The present study clearly demonstrates that comparing results from various sources is difficult even when the results are achieved under seemingly similar conditions. The appeal for an unified experimental procedure for the investigation of processes in Fe(0)/H(2)O systems is reiterated.

  14. Environmental applications of poplars and willows

    Science.gov (United States)

    J.G. Isebrands; P. Aronsson; M. Carlson; R. Ceulemans; M. Coleman; N. Dickinson; J. Dimitriou; S. Doty; E. Gardiner; K. Heinsoo; J.D. Johnson; Y.B. Koo; J. Kort; J. Kuzovkina; L. Licht; A.R. McCracken; I. McIvor; P. Mertens; K. Perttu; D. Riddell-Black; B. Robins; G. Scarascia-Mugnozza; W.R. Schroeder; John Stanturf; T.A. Volk; M. Weih

    2014-01-01

    Poplars and willows have been planted for environmental purposes for millennia. There are reports that poplars were planted to improve the human environment 4000 years ago in the third dynasty of Ur, for streamside stabilization 2000 years ago in what is now the south-western USA by native North Americans and for urban amenities by the early Chinese dynasties (see...

  15. Optimization of Paper Discoloration via Pyrolysis Using Lasers

    KAUST Repository

    Alhashem, Mayadah M.

    2017-04-01

    Printing ink is a main component of the modern printer, and it has always been throughout the history of printing. Ink and toners are expensive replaceable components that inkjet and laser printers cannot function without. The digital printing industry, which is majorly composed of monochrome printing, is expected to increase by 225% by 2024 from a 2013 baseline (Smithers et al., 2014). Expenses aside, toner cartridges and ink cartridges pose an overlooked threat to the environment. Manufacturing, packaging, transporting, and waste disposal of printer ink and toners result in carbon dioxide emissions. The complete elimination of ink in monochrome printing is potentially viable with the patented new discoloration technique. The patent studies a discoloration method by carbonizing a paper’s surface (Alhashem et al., 2015). The printing method optimizes surface paper pyrolysis via laser heating. The aim is obtaining the darkest possible shade without compromising paper quality. The challenge is in creating a printed area from the paper material itself, rather than depositing ink on paper. A 75-watt CO2 laser engraving machine emitting a 10.6 μm wavelength beam for heating is used with low power settings to carbonize a fraction of the paper surface. The carbonization is essentially a combustion reaction. Solid fuel burns in three stages: drying, devolatilization (pyrolysis, or distillation phase), and lastly, the char (charcoal) combustion. These stages are driven by heat from the CO2 laser. Moving the laser rapidly above the paper surface arrests the reaction at the second stage, after the formation of blackened char. The control variables in the experimental method are laser power, speed, and the vertical position that affects the laser intensity. Computer software controls these variables. The discoloration of paper is quantified by measuring the light absorptivity using a UV-Vis-IR Spectrometer.

  16. 16S partial gene mitochondrial DNA and internal transcribed spacers ribosomal DNA as differential markers of Trichuris discolor populations.

    Science.gov (United States)

    Callejón, R; Halajian, A; de Rojas, M; Marrugal, A; Guevara, D; Cutillas, C

    2012-05-25

    Comparative morphological, biometrical and molecular studies of Trichuris discolor isolated from Bos taurus from Spain and Iran was carried out. Furthermore, Trichuris ovis isolated from B. taurus and Capra hircus from Spain has been, molecularly, analyzed. Morphological studies revealed clear differences between T. ovis and T. discolor isolated from B. taurus but differences were not observed between populations of T. discolor isolated from different geographical regions. Nevertheless, the molecular studies based on the amplification and sequencing of the internal transcribed spacers 1 and 2 ribosomal DNA and 16S partial gene mitochondrial DNA showed clear differences between both populations of T. discolor from Spain and Iran suggesting two cryptic species. Phylogenetic studies corroborated these data. Thus, phylogenetic trees based on ITS1, ITS2 and 16S partial gene sequences showed that individuals of T. discolor from B. taurus from Iran clustered together and separated, with high bootstrap values, of T. discolor isolated from B. taurus from Spain, while populations of T. ovis from B. taurus and C. hircus from Spain clustered together but separated with high bootstrap values of both populations of T. discolor. Furthermore, a comparative phylogenetic study has been carried out with the ITS1and ITS2 sequences of Trichuris species from different hosts. Three clades were observed: the first clustered all the species of Trichuris parasitizing herbivores (T. discolor, T. ovis, Trichuris leporis and Trichuris skrjabini), the second clustered all the species of Trichuris parasitizing omnivores (Trichuris trichiura and Trichuris suis) and finally, the third clustered species of Trichuris parasitizing carnivores (Trichuris muris, Trichuris arvicolae and Trichuris vulpis). Copyright © 2011 Elsevier B.V. All rights reserved.

  17. The potential of willow and poplar plantations as carbon sinks in Sweden

    International Nuclear Information System (INIS)

    Rytter, Rose-Marie

    2012-01-01

    A large share, estimated at 12–25%, of the annual anthropogenic greenhouse gas emissions is attributed to global deforestation. Increasing the forested areas therefore has a positive impact on carbon (C) sequestration and mitigation of high atmospheric CO 2 concentrations. Fast-growing species, such as willow and poplar, are of high interest as producers of biomass for fuel, but also as C sinks. The present study estimated the rate of C sequestration in biomass and soil in willow and poplar plantations. Calculations were based on above- and below-ground biomass production data from field experiments, including fine root turnover, litter decomposition rates, and production levels from commercial plantations. Accumulation of C in woody biomass, above and below ground, was estimated at 76.6–80.1 Mg C ha −1 and accumulation of C in the soil at 9.0–10.3 Mg C ha −1 over the first 20–22 years. The average rates of C sequestration were 3.5–4.0 Mg C ha −1 yr −1 in woody biomass, and 0.4–0.5 Mg C ha −1 yr −1 in the soil. If 400,000 ha of abandoned arable land in Sweden were planted with willow and poplar, about 1.5 Tg C would be sequestered annually in woody biomass and 0.2 Tg C in soils. This would be nearly one tenth of the annual anthropogenic emissions of C in Sweden today. These calculations show the potential of fast-growing plantations on arable land to mitigate the effect of high CO 2 concentrations over a short time span. Knowledge gaps were found during the calculation process and future research areas were suggested. -- Highlights: ► Poplars and willows as producers of biomass for fuel and as C sinks. ► Calculation of C sequestration rates in biomass and soil in willow and poplar plantations. ► Increasing forested areas has positive impact on high CO 2 levels. ► Willow and poplar plantations on arable land mitigate anthropogenic CO 2 emissions.

  18. Combined production of free-range pigs and energy crops – animal behaviour and crop damages

    DEFF Research Database (Denmark)

    Horsted, Klaus; Kongsted, Anne Grete; Jørgensen, Uffe

    2012-01-01

    Intensive free-range pig production on open grasslands has disadvantages in that it creates nutrient hotspots and little opportunity for pigs to seek shelter from the sun. Combining a perennial energy crop and pig production might benefit the environment and animal welfare because perennial energy...... crops like willow (Salix sp.) and Miscanthus offer the pigs protection from the sun while reducing nutrient leaching from pig excrements due to their deep rooting system. The objectives of this study were to evaluate how season and stocking density of pigs in a free-range system with zones of willow...

  19. Seasonal Dynamics of Water Use Strategy of Two Salix Shrubs in Alpine Sandy Land, Tibetan Plateau.

    Science.gov (United States)

    Zhu, Yajuan; Wang, Guojie; Li, Renqiang

    2016-01-01

    Water is a limiting factor for plant growth and vegetation dynamics in alpine sandy land of the Tibetan Plateau, especially with the increasing frequency of extreme precipitation events and drought caused by climate change. Therefore, a relatively stable water source from either deeper soil profiles or ground water is necessary for plant growth. Understanding the water use strategy of dominant species in the alpine sandy land ecosystem is important for vegetative rehabilitation and ecological restoration. The stable isotope methodology of δD, δ18O, and δ13C was used to determine main water source and long-term water use efficiency of Salix psammophila and S. cheilophila, two dominant shrubs on interdune of alpine sandy land in northeastern Tibetan Plateau. The root systems of two Salix shrubs were investigated to determine their distribution pattern. The results showed that S. psammophila and S. cheilophila absorbed soil water at different soil depths or ground water in different seasons, depending on water availability and water use strategy. Salix psammophila used ground water during the growing season and relied on shallow soil water recharged by rain in summer. Salix cheilophila used ground water in spring and summer, but relied on shallow soil water recharged by rain in spring and deep soil water recharged by ground water in fall. The two shrubs had dimorphic root systems, which is coincident with their water use strategy. Higher biomass of fine roots in S. psammophila and longer fine roots in S. cheilophila facilitated to absorb water in deeper soil layers. The long-term water use efficiency of two Salix shrubs increased during the dry season in spring. The long-term water use efficiency was higher in S. psammophila than in S. cheilophila, as the former species is better adapted to semiarid climate of alpine sandy land.

  20. Biomass production and control of nutrient leaching of willows using different planting methods with special emphasis on an appraisal of the electrical impedance for roots

    Energy Technology Data Exchange (ETDEWEB)

    Yang Cao

    2011-07-01

    Willow reproduction can be achieved through vertically or horizontally planted cuttings. Conventionally, plantations are established by inserting cuttings vertically into the soil. There is, however, a lack of information about the biomass production and nutrient leaching of plantations established through horizontally planted cuttings. A greenhouse experiment and a field trial were carried out to investigate whether horizontally planted Salix schwerinii cuttings have a positive effect on stem yield, root distribution and nutrient leaching in comparison with vertically planted cuttings with different planting densities. The shoots' height of horizontally planted cuttings was significantly smaller than that of vertically planted cuttings during the first two weeks after planting in the pot experiment. Thereafter, no significant effect of planting orientation on the stem biomass was observed in the two conducted experiments. In both experiments the total stem biomass increased with the planting density. It was also found that the fine root biomass and the specific root length were not affected by the planting orientation or density, while the fine root surface area and the absorbing root surface area (ARSA) were affected only by the planting density. The planting orientation did not affect the nutrient concentrations in the soil leachate, apart from SO{sub 4}-S and PO{sub 4}-P in the pot experiment. The ARSA in the pot experiment was assessed by using the earth impedance method. The applicability of this method was evaluated in a hydroponic study of willow cuttings where root and stem were measured independently. Electrical resistance had a good correlation with the contact area of the roots with the solution. However, the resistance depended strongly on the contact area of the stem with the solution, which caused a bias in the evaluation of root surface area. A similar experimental set-up with electrical impedance spectroscopy was employed to study the

  1. The potential of two Salix genotypes for radionuclide/heavy metal accumulation. A case study of Rovinari ash pit (Gorj District, Romania)

    Science.gov (United States)

    Hernea, Cornelia; Neţoiu, Constantin; Corneanu, Gabriel; Crăciun, Constantin; Corneanu, Mihaela; Cojocaru, Luminiţa; Rovena Lăcătuşu, Anca; Popescu, Ion

    2014-05-01

    Thermo Electric Power Plants (TEPP) produce a high amount of ash, that contains heavy metals and radionuclides. Ash is usually stored in ash-pits, in mixture with water and contains U235, Th 234 and their decay products, that are released from the coal matrix, during combustion, as well as heavy metals. Warm weather dried the ash and it can be spread by the wind in surrounded area. This paper presents the results of an experiment with two Salix genotypes, cultivated on an old closed ash-pit, nearby the Rovinari TEPP, in the middle Jiu valley (Gorj District, Romania), in order to evaluate its tolerance to heavy metals and radionuclides. Ash analysis revealed the presence of natural radionuclides, beloging from ash and coal dust, as well as of Cs 137, of Chernobil provenance. Radionuclides content over the normal limits for Romania were registered for Th 234, Pb210, U235 and Ra226. The heavy metals level in ash was over the normal limits, but under the alerts limits. In order to establish the woody plants tolerance to heavy metals and radionuclides, it is important to study the seedlings behavior. In this respect Salix alba and Salix viminalis whips and cuttings culture have been establish on Rovinari ash-pit. The observations made on survival and growth rate pointed out the superiority of Salix viminalis behaviour. After a period of three years Salix viminalis registered a 96% survival rate, while in Salix alba annual decreases, reaching to 14%. These results are supported by the radionuclides content in leaves and by the electron microscopy studies. In Salix alba the leaves parenchimatic cells present a low sinthesis activity. The exogenous particles are accumulated in parenchima cells vacuola, the chloroplasts are usually agranal, with few starch grains and mitocondria presents slightly dillated crista. The ultrastructural features of the mature leaf cells, evidenced the natural adaptation of Salix viminalins for development in an environment with a big amount of

  2. Contrasting the community structure of arbuscular mycorrhizal fungi from hydrocarbon-contaminated and uncontaminated soils following willow (Salix spp. L. planting.

    Directory of Open Access Journals (Sweden)

    Saad El-Din Hassan

    Full Text Available Phytoremediation is a potentially inexpensive alternative to chemical treatment of hydrocarbon-contaminated soils, but its success depends heavily on identifying factors that govern the success of root-associated microorganisms involved in hydrocarbon degradation and plant growth stimulation. Arbuscular mycorrhizal fungi (AMF form symbioses with many terrestrial plants, and are known to stimulate plant growth, although both species identity and the environment influence this relationship. Although AMF are suspected to play a role in plant adaptation to hydrocarbon contamination, their distribution in hydrocarbon-contaminated soils is not well known. In this study, we examined how AMF communities were structured within the rhizosphere of 11 introduced willow cultivars as well as unplanted controls across uncontaminated and hydrocarbon-contaminated soils at the site of a former petrochemical plant. We obtained 69 282 AMF-specific 18S rDNA sequences using 454-pyrosequencing, representing 27 OTUs. Contaminant concentration was the major influence on AMF community structure, with different AMF families dominating at each contaminant level. The most abundant operational taxonomic unit in each sample represented a large proportion of the total community, and this proportion was positively associated with increasing contamination, and seemingly, by planting as well. The most contaminated soils were dominated by three phylotypes closely related to Rhizophagus irregularis, while these OTUs represented only a small proportion of sequences in uncontaminated and moderately contaminated soils. These results suggest that in situ inoculation of AMF strains could be an important component of phytoremediation treatments, but that strains should be selected from the narrow group that is both adapted to contaminant toxicity and able to compete with indigenous AMF species.

  3. Contrasting the community structure of arbuscular mycorrhizal fungi from hydrocarbon-contaminated and uncontaminated soils following willow (Salix spp. L.) planting.

    Science.gov (United States)

    Hassan, Saad El-Din; Bell, Terrence H; Stefani, Franck O P; Denis, David; Hijri, Mohamed; St-Arnaud, Marc

    2014-01-01

    Phytoremediation is a potentially inexpensive alternative to chemical treatment of hydrocarbon-contaminated soils, but its success depends heavily on identifying factors that govern the success of root-associated microorganisms involved in hydrocarbon degradation and plant growth stimulation. Arbuscular mycorrhizal fungi (AMF) form symbioses with many terrestrial plants, and are known to stimulate plant growth, although both species identity and the environment influence this relationship. Although AMF are suspected to play a role in plant adaptation to hydrocarbon contamination, their distribution in hydrocarbon-contaminated soils is not well known. In this study, we examined how AMF communities were structured within the rhizosphere of 11 introduced willow cultivars as well as unplanted controls across uncontaminated and hydrocarbon-contaminated soils at the site of a former petrochemical plant. We obtained 69 282 AMF-specific 18S rDNA sequences using 454-pyrosequencing, representing 27 OTUs. Contaminant concentration was the major influence on AMF community structure, with different AMF families dominating at each contaminant level. The most abundant operational taxonomic unit in each sample represented a large proportion of the total community, and this proportion was positively associated with increasing contamination, and seemingly, by planting as well. The most contaminated soils were dominated by three phylotypes closely related to Rhizophagus irregularis, while these OTUs represented only a small proportion of sequences in uncontaminated and moderately contaminated soils. These results suggest that in situ inoculation of AMF strains could be an important component of phytoremediation treatments, but that strains should be selected from the narrow group that is both adapted to contaminant toxicity and able to compete with indigenous AMF species.

  4. Biomass power for rural development. Technical progress report, October 1--December 31, 1997

    Energy Technology Data Exchange (ETDEWEB)

    Neuhauser, E.

    1998-05-01

    The focus of the DOE/USDA sponsored biomass power for rural development project is to develop commercial energy crops for power generation by the year 2000. The New York based Salix Consortium project is a multi-partner endeavor, implemented in three stages. Phase-1, Final Design and Project Development, will conclude with the preparation of construction and/or operating permits, feedstock production plans, and contracts ready for signature. Field trials of willow (Salix) have been initiated at several locations in New York (Tully, Lockport, King Ferry, La Fayette, Massena, and Himrod) and co-firing tests are underway at Greenidge Station (NYSEG) and Dunkirk Station (NMPC). Phase-2 of the project will focus on scale-up of willow crop acreage, construction of co-firing facilities at Dunkirk Station (NMPC), and final modifications for Greenidge Station. Cofiring willow is also under consideration for GPU`s Seward Station where testing is underway. There will be an evaluation of the energy crop as part of the gasification trials occurring at BED`s McNeill Power Station. Phase-3 will represent fullscale commercialization of the energy crop and power generation on a sustainable basis. During the fourth quarter of 1997 the Consortium submitted a Phase-2 proposal. A few of the other more important milestones are outlined below. The first quarter of 1998 will be dominated by pre-planting activity in the spring.

  5. Photo-bleaching of polymer discoloration caused by quinone methides

    Czech Academy of Sciences Publication Activity Database

    Pospíšil, Jan; Nešpůrek, Stanislav; Zweifel, H.; Kuthan, J.

    2002-01-01

    Roč. 78, č. 2 (2002), s. 251-255 ISSN 0141-3910 R&D Projects: GA AV ČR IAA1050901 Institutional research plan: CEZ:AV0Z4050913 Keywords : quinone methide * photo- bleaching * polymer discoloration Subject RIV: CD - Macromolecular Chemistry Impact factor: 1.145, year: 2002

  6. Selecting and utilizing Populus and Salix for landfill covers: implications for leachate irrigation.

    Science.gov (United States)

    Zalesny, Ronald S; Bauer, Edmund O

    2007-01-01

    The success of using Populus and Salix for phytoremediation has prompted further use of leachate as a combination of irrigation and fertilization for the trees. A common protocol for such efforts has been to utilize a limited number of readily-available genotypes with decades of deployment in other applications, such as fiber or windbreaks. However, it may be possible to increase phytoremediation success with proper genotypic screening and selection, followed by the field establishment of clones that exhibited favorable potential for cleanup of specific contaminants. There is an overwhelming need for testing and subsequent deployment of diverse Populus and Salix genotypes, given current availability of clonal material and the inherent genetic variation among and within these genera. Therefore, we detail phyto-recurrent selection, a method that consists of revising and combining crop and tree improvement protocols to meet the objective of utilizing superior Populus and Salix clones for remediation applications. Although such information is lacking for environmental clean-up technologies, centuries of plant selection success in agronomy, horticulture, and forestry validate the need for similar approaches in phytoremediation. We bridge the gap between these disciplines by describing project development, clone selection, tree establishment, and evaluation of success metrics in the context of their importance to utilizing trees for phytoremediation.

  7. Available data support protection of the Southwestern Willow Flycatcher under the Endangered Species Act

    Science.gov (United States)

    Theimer, Tad C.; Smith, Aaron D.; Mahoney, Sean M.; Ironside, Kirsten E.

    2016-01-01

    Zink (2015) argued there was no evidence for genetic, morphological, or ecological differentiation between the federally endangered Southwestern Willow Flycatcher (Empidonax traillii extimus) and other Willow Flycatcher subspecies. Using the same data, we show there is a step-cline in both the frequency of a mtDNA haplotype and in plumage variation roughly concordant with the currently recognized boundary between E. t. extimus and E. t adastus, the subspecies with which it shares the longest common boundary. The geographical pattern of plumage variation is also concordant with previous song analyses differentiating those 2 subspecies and identified birds in one low-latitude, high-elevation site in Arizona as the northern subspecies. We also demonstrate that the ecological niche modeling approach used by Zink yields the same result whether applied to the 2 flycatcher subspecies or to 2 unrelated species, E. t. extimus and Yellow Warbler (Setophaga petechia). As a result, any interpretation of those results as evidence for lack of ecological niche differentiation among Willow Flycatcher subspecies would also indicate no differentiation among recognized species and would therefore be an inappropriate standard for delineating subspecies. We agree that many analytical techniques now available to examine genetic, morphological, and ecological differentiation would improve our understanding of the distinctness (or lack thereof) of Willow Flycatcher subspecies, but we argue that currently available evidence supports protection of the Southwestern Willow Flycatcher under the Endangered Species Act.

  8. DESIGNING AN OPPORTUNITY FUEL WITH BIOMASS AND TIRE-DERIVED FUEL FOR COFIRING AT WILLOW ISLAND GENERATING STATION

    International Nuclear Information System (INIS)

    K. Payette; D. Tillman

    2001-01-01

    During the period October 1, 2000 - December 31, 2000, Allegheny Energy Supply Co., LLC (Allegheny) executed a Cooperative Agreement with the National Energy Technology Laboratory to implement a major cofiring demonstration at the Willow Island Generating Station Boiler No.2. Willow Island Boiler No.2 is a cyclone boiler. Allegheny also will demonstrate separate injection cofiring at the Albright Generating Station Boiler No.3, a tangentially fired boiler. The Allegheny team includes Foster Wheeler as its primary subcontractor. Additional subcontractors are Cofiring Alternatives and N.S. Harding and Associates. This report summarizes the activities associated with the Designer Opportunity Fuel program, and demonstrations at Willow Island and Albright Generating Stations. The second quarter of the project involved completing the designs for each location. Further, geotechnical investigations proceeded at each site. Preparations were made to perform demolition on two small buildings at the Willow Island site. Fuels strategies were initiated for each site. Test planning commenced for each site. A groundbreaking ceremony was held at the Willow Island site on October 18, with Governor C. Underwood being the featured speaker

  9. Declines in populations of Salix caprea L.during forest regeneration after strong herbivore pressure

    Directory of Open Access Journals (Sweden)

    Janusz B. Faliński

    2014-01-01

    Full Text Available Communities of broadleaved forest subject to strong pressure from large herbivores underwent degeneration. The relief of this pressure led to regeneration of the community, in which an important role was played by the sallow Salix caprea and other light-seeded pioneer species of tree (Populus tremula, Betula pendula and B. pubescens. Regeneration involving Salix caprea proceeded following the conservatorial protection of the degenerate stands in a reserve and later in Białowieża National Park. The emergence and development of the population of Salix caprea proceeded following the invasion of spruce, which coincided with the period of enhanced animal pressure on broadleaved forest. Salix caprea filled all the gaps in the tree stand arising as a result of the destruction of trees and undergrowth by herbivores (in the years 1892-1915. It also appeared en masse on old, at that time unforested, clearings and felled areas. In these places, Salix caprea created very abundant populations, with particular trees being in good condition, with a habit typical of forest trees and attaining considerable heights. The majority of trees were 50-60 years old at the time of death, although individuals reached 74 years of age. The process of extinction of the sallow population - observed over 19 years on permanent plots and fixed trees - proceeded very quickly, especially in the first decade of observation. It led to the almost complete disappearance of sallow for the forest communities of Białowieża National Park. The death of individual trees is preceded by impairment of their health and reduced annual increments in the 4-9 last years of life. The extinction of the population is associated with the loss of its primary phenological differentiation and with a change in the sex structure of the population from a prevalence of female trees to a near even distribution of the two sexes. The development of the populations of permanent constituents of the forest

  10. A comparative analysis of genetic differentiation across six shared willow host species in leaf- and bud-galling sawflies.

    Directory of Open Access Journals (Sweden)

    Sanna A Leppänen

    Full Text Available Genetic divergence and speciation in plant-feeding insects could be driven by contrasting selection pressures imposed by different plant species and taxa. While numerous examples of host-associated differentiation (HAD have been found, the overall importance of HAD in insect diversification remains unclear, as few studies have investigated its frequency in relation to all speciation events. One promising way to infer the prevalence and repeatability of HAD is to estimate genetic differentiation in multiple insect taxa that use the same set of hosts. To this end, we measured and compared variation in mitochondrial COI and nuclear ITS2 sequences in population samples of leaf-galling Pontania and bud-galling Euura sawflies (Hymenoptera: Tenthredinidae collected from six Salix species in two replicate locations in northern Fennoscandia. We found evidence of frequent HAD in both species complexes, as individuals from the same willow species tended to cluster together on both mitochondrial and nuclear phylogenetic trees. Although few fixed differences among the putative species were found, hierarchical AMOVAs showed that most of the genetic variation in the samples was explained by host species rather than by sampling location. Nevertheless, the levels of HAD measured across specific pairs of host species were not correlated in the two focal galler groups. Hence, our results support the hypothesis of HAD as a central force in herbivore speciation, but also indicate that evolutionary trajectories are only weakly repeatable even in temporally overlapping radiations of related insect taxa.

  11. Comparing predicted yield and yield stability of willow and Miscanthus across Denmark

    DEFF Research Database (Denmark)

    Larsen, Søren; Jaiswal, Deepak; Bentsen, Niclas Scott

    2016-01-01

    was 12.1 Mg DM ha−1 yr−1 for willow and 10.2 Mg DM ha−1 yr−1 for Miscanthus. Coefficent of variation as a measure for yield stability was poorest on the sandy soils of northern and western Jutland and the year-to-year variation in yield was greatest on these soils. Willow was predicted to outyield...... Miscanthus on poor, sandy soils whereas Miscanthus was higher yielding on clay-rich soils. The major driver of yield in both crops was variation in soil moisture, with radiation and precipitation exerting less influence. This is the first time these two major feedstocks for northern Europe have been compared....... The semi-mechanistic crop model BioCro was used to simulate the production of both short rotation coppice (SRC) willow and Miscanthus across Denmark. Predictions were made from high spatial resolution soil data and weather records across this area for 1990-2010. The potential average, rain-fed mean yield...

  12. Surface photo-discoloration and degradation of dyed wood veneer exposed to different wavelengths of artificial light

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Yi [MOE Key Laboratory of Wooden Material Science and Application, Beijing Forestry University, Beijing 100083 (China); Beijing Key Laboratory of Wood Science and Engineering, Beijing Forestry University, Beijing 100083 (China); MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083 (China); Forest Products Development Center, School of Forestry and Wildlife Sciences, Auburn University, Auburn, AL 36830 (United States); Shao, Lingmin [MOE Key Laboratory of Wooden Material Science and Application, Beijing Forestry University, Beijing 100083 (China); Gao, Jianmin, E-mail: jmgao@bjfu.edu.cn [MOE Key Laboratory of Wooden Material Science and Application, Beijing Forestry University, Beijing 100083 (China); Beijing Key Laboratory of Wood Science and Engineering, Beijing Forestry University, Beijing 100083 (China); MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083 (China); Guo, Hongwu, E-mail: hwg5052@163.com [MOE Key Laboratory of Wooden Material Science and Application, Beijing Forestry University, Beijing 100083 (China); Beijing Key Laboratory of Wood Science and Engineering, Beijing Forestry University, Beijing 100083 (China); MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083 (China); Chen, Yao [MOE Key Laboratory of Wooden Material Science and Application, Beijing Forestry University, Beijing 100083 (China); Beijing Key Laboratory of Wood Science and Engineering, Beijing Forestry University, Beijing 100083 (China); MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083 (China); Cheng, Qingzheng; Via, Brian K. [Forest Products Development Center, School of Forestry and Wildlife Sciences, Auburn University, Auburn, AL 36830 (United States)

    2015-03-15

    Highlights: • Investigate the selective absorption of different wavelengths of UV–vis light by dyed wood chromophores. • Identify connection between light wavelengths and surface color changes and chemical structure degradation. • Study hypochromic effect based on surface reflectance and K/S absorption changes during UV–vis irradiation. - Abstract: The surface of dyed wood is prone to discoloration when exposed to light irradiation which significantly decreases its decorative effect and shortens its service life. The influence of light wavelength exposure to the surface of dyed wood was investigated to study the effect on discoloration and degradation. Acid Blue V and Acid Red GR dyed wood veneers were subjected to light exposure with different wavelengths from the UV to visible region (254–420 nm). Results showed that the surface discoloration of dyed wood was linearly related to lignin concentration and dyes degradation and the consequent transformation of chromophoric groups such as aromatic (C=C) and carbonyl (C=O) through methoxy reaction. The dyes, lignin and some active constituents were degraded severely, even at short exposures. Acid Blue V dyed wood exhibited greater discoloration than the Acid Red GR treatment. The reflectance and K/S absorption curve showed a hypochromic effect on the dyed wood surface. The dyes and wood chemical structure played a complex and combined role on the selective absorption of different wavelengths of light. The color change rate was apparent with 254 nm exposure in the initial stages, but a greater discoloration rate occurred on the samples irradiated at 313 and 340 nm than at 254 and 420 nm with the time prolonged. The degradation rate and degree of discoloration correlated well with the light energy and wavelength.

  13. Greenhouse gas emissions from willow-based electricity: a scenario analysis for Portugal and The Netherlands

    NARCIS (Netherlands)

    Rebelo de Mira, R.; Kroeze, C.

    2006-01-01

    This study focuses on greenhouse gas emissions from power plants using willow as fuel compared to those using fossil fuels. More specifically, we quantify emissions of nitrous oxide (N2O) from soils on which willow is grown, and compare these to emissions of carbon dioxide (CO2) from fossil

  14. Impact of abiotic factors on frost resistance and cold acclimation in Salix species and clones

    Energy Technology Data Exchange (ETDEWEB)

    Fircks, H. von [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Short Rotation Forestry

    1996-12-31

    The effects of mineral nitrogen, photoperiod and day-night temperature on frost resistance and growth cessation in Salix species and clones are discussed. Increased nitrogen supply and imbalances between nitrogen and other elements might cause extensive frost damage in plants of Salix. Vegetation frosts below -3 deg C reduces the level of annual yield. Although Salix clones differ in resistance to freezing stress, the capacity to recover and grow after frosts are equal essential properties which affect the growth and biomass production of shoots after night frosts in June. Early autumn frosts causing freezing damage not only may delay the onset of growth cessation and cold acclimation, but also affect the winter survival of shoots. Increased nitrogen supply prior to cold acclimation postponed growth cessation and cold acclimation. Differences in nutrient status in plants cause also differences in retranslocation of mineral nutrients. Absence of damaging autumn frosts allow plants irrespective of nitrogen status to develop a frost resistance of at least - 80 deg C. 21 refs, 1 fig, 3 tabs

  15. Color recovery effect of different bleaching systems on a discolored composite resin.

    Science.gov (United States)

    Gul, P; Harorlı, O T; Ocal, I B; Ergin, Z; Barutcigil, C

    2017-10-01

    Discoloration of resin-based composites is a commonly encountered problem, and bleaching agents may be used for the therapy of the existing discoloration. The purpose of this study was to investigate in vitro color recovery effect of different bleaching systems on the heavily discolored composite resin. Fifty disk-shaped dental composite specimens were prepared using A2 shade nanohybrid universal composite resin (3M ESPE Filtek Z550, St. Paul, MN, USA). Composite samples were immersed in coffee and turnip juice for 1 week in each. One laser activated bleaching (LB) (Biolase Laserwhite*20) and three conventional bleaching systems (Ultradent Opalescence Boost 40% (OB), Ultradent Opalescence PF 15% home bleaching (HB), Crest 3D White [Whitening Mouthwash]) were tested in this study. Distilled water was used as control group. The color of the samples were measured using a spectrophotometer (VITA Easy shade Compact, VITA Zahnfabrik, Bad Säckingen, Germany). Color changes (ΔE00) were calculated using the CIEDE2000 formula. Statistical analyses were conducted using paired samples test, one-way analysis of variance, and Tukey's multiple comparison tests (α = 0.05). The staining beverages caused perceptible discoloration (ΔE00 > 2.25). The color recovery effect of all bleaching systems was statistically determined to be more effective than the control group (P OB group was found as the most effective bleaching system, there was no statistically significant difference among HB, OB, and LB groups (P > 0.05). Within the limitation of this in vitro study, the highest recovery effect was determined in office bleaching system among all bleaching systems. However, home and laser bleaching systems were determined as effective as office bleaching system.

  16. The role of the embryo and ethylene in avocado fruit mesocarp discoloration

    Science.gov (United States)

    Hershkovitz, Vera; Friedman, Haya; Goldschmidt, Eliezer E.; Pesis, Edna

    2009-01-01

    Chilling injury (CI) symptoms in avocado (Persea americana Mill.) fruit, expressed as mesocarp discoloration, were found to be associated with embryo growth and ethylene production during cold storage. In cvs Ettinger and Arad most mesocarp discoloration was located close to the base of the seed and was induced by ethylene treatment in seeded avocado fruit. However, ethylene did not increase mesocarp discoloration in seedless fruit stored at 5 °C. Application of ethylene to whole fruit induced embryo development inside the seed. It also induced seedling elongation when seeds were imbibed separately. Persea americana ethylene receptor (PaETR) gene expression and polyphenol oxidase activity were highest close to the base of the seed and decreased gradually toward the blossom end. By contrast, expressions of PaETR transcript and polyphenol oxidase activity in seedless avocado fruit were similar throughout the pulp at the base of the fruit. Application of the ethylene inhibitor, 1-methylcyclopropene, decreased mesocarp browning, embryo development, seedling growth, and ion leakage, and down-regulated polyphenol oxidase activity. The results demonstrate that ethylene-mediated embryo growth in whole fruit is involved in the mesocarp response to ethylene perception and the development of CI disorders. PMID:19196750

  17. Evaluation of the Effect of Different Herbal Tea on Discoloration of Different Kinds of Composite

    Directory of Open Access Journals (Sweden)

    Fatemeh Nezhadnasrollah

    2016-09-01

    Full Text Available Objectives: The aim of this study was to evaluate the effect of six herbal teas on the color stability of two types of nanohybrid and one microhybrid resin composite.  Materials and Methods: 70 disc-shaped specimens, 210 in total (7*2mm, were fabricated from each of the following materials in metal mould : Tetric N ceram, Grandio, Gradia Direct Anterior. Specimens were stored in distilled water at 37°C for 24 hours in an incubator for completion of polymerization. After baseline evaluation (L*, a*, b*CIELAB scale, the specimens were divided into seven subgroups, according to the test and control storage solutions (n=10. Randomly selected specimens from each material were immersed in 20 ml of the test solutions (Borago, Green, Hibiscus, Thyme, Black and Lemon Verbena teas at 37˚c for 24 hours and 48 hours. Solutions were refreshed every 24 hours. All samples were polished using Soflex discs with Medium, Fine, Superfine grit after storage in herbal teas. Specimens color was measured in 24, 48 hours and after polishing. The collected data was statistically analyzed using two-way analysis of variance with repeated measure and Tukey’s HSD at a significance level of 0.05. Results: All samples displayed color changes after immersion in the herbal teas. Hibiscus tea induced the highest level of discoloration after 24 hours immersion in all three composites. Black tea induced highest level of discoloration in (Grandio ΔE=7.44. Hibiscus tea and Thyme tea induced highest level of discoloration in (Tetric N ceram ΔE=11. and (Gradia Direct ΔE=14.11, respectively, after 48 hours immersion. The least discoloration was found with Borage tea in 24 and 48 hours. After re-polishing the color change was reduced. Grandio showed the greatest color reduction in Black tea. Color improvement of Tetric N ceram was better than Gradia Direct. Conclusion: All tested restorative materials showed a color shift after immersion in herbal teas, which Tetric N ceram

  18. Sex and the single Salix: considerations for riparian restoration

    Science.gov (United States)

    Thomas D. Landis; David R. Dreesen; R. Kasten Dumroese

    2003-01-01

    Most restoration projects strive to create a sustain able plant community but exclusive use of vegetatively propagated material may be preventing this goal. The dioecious willows and cottonwoods of the Salicaceae are widely used in riparian restoration projects. Hardwood cuttings have traditionally been used to propagate these species in nurseries, and live stakes,...

  19. Wind in the Willows--Theatre Activity Packet.

    Science.gov (United States)

    New York City Board of Education, Brooklyn, NY. Div. of Curriculum and Instruction.

    Part of the New York City Board of Education's Early Stages program, and intended for elementary and secondary school teachers who wish to include a unit on theater in their classes, this guide offers suggestions for lessons and activities to accompany viewing a performance of "Wind in the Willows" at the Nederlander Theater. Part one of…

  20. Allium Discoloration: Color Compounds Formed during Pinking of Onion and Leek

    Czech Academy of Sciences Publication Activity Database

    Kubec, R.; Urajová, P.; Lacina, O.; Hajšlová, J.; Kuzma, Marek; Zápal, Jakub

    2015-01-01

    Roč. 63, č. 46 (2015), s. 10192-10199 ISSN 0021-8561 Institutional support: RVO:61388971 Keywords : discoloration * plant pigment * pinking Subject RIV: CE - Biochemistry Impact factor: 2.857, year: 2015

  1. To be or not to be a subspecies: description of Saperda populnea lapponica ssp. n. (Coleoptera, Cerambycidae) developing in downy willow (Salix lapponum L.)

    Science.gov (United States)

    Wallin, Henrik; Kvamme, Torstein; Bergsten, Johannes

    2017-01-01

    Abstract A new subspecies of the European cerambycid Saperda populnea (Linnaeus, 1758) is described: Saperda populnea lapponica ssp. n. based on specimens from Scandinavia. The male genitalia characters were examined and found to provide support for this separation, as well as differences in morphology, geographical distribution and bionomy. The preferred host tree for the nominate subspecies S. populnea populnea is Populus tremula L., whereas S. populnea lapponica ssp. n. is considered to be monophagous on Salix lapponum L. DNA sequence data of mitochondrial cytochrome oxidase subunit I (COI) was generated from Scandinavian specimens of S. populnea populnea and specimens representing S. populnea lapponica ssp. n. The two subspecies were not reciprocally monophyletic and genetic distances in COI were small. All synonyms of S. populnea populnea have been considered, and species similar to S. populnea populnea have been examined, and not found to be related to S. populnea lapponica ssp. n. A male lectotype has been designated for each of the two following synonyms: Cerambyx decempunctatus De Geer, 1775, and Saperda salicis Zetterstedt, 1818. The synonymised species from Asia, S. balsamifera (Motshulsky, 1860), is elevated to subspecies: S. populnea balsamifera stat. n. We end with a discussion on the definition of subspecies under the unified species concept. PMID:29187784

  2. GIS-BASED location optimization of a biomass conversion plant on contaminated willow in the Campine region (Belgium)

    International Nuclear Information System (INIS)

    Voets, Thomas; Neven, An; Thewys, Theo; Kuppens, Tom

    2013-01-01

    The Campine region is diffusely contaminated with heavy metals like cadmium. Since traditional excavation techniques are too expensive, phytoremediation is preferred as a remediation technique. In a previous study, the biomass potential from phytoremediation of contaminated agricultural land in the Campine region in Belgium was assessed. Based on recently upgraded figures of willow potential from phytoremediation on agricultural land in the seven most contaminated municipalities of the Belgian Campine region, the current paper uses GIS-knowledge to investigate which of three previously identified locations is most suitable for a biomass plant, taking into account the spatial distribution of the contaminated willow supply and the total cost of willow transport. Biomass transport distance from the centroid of each contaminated agricultural parcel to each of the three potential biomass plant locations was determined following Euclidian distance calculations and distance calculations over the existing road network. A transport cost model consisting of distance fixed and distance dependent biomass transport costs was developed. Of the locations identified, the Overpelt Fabriek site results in the lowest biomass transport distance and costs. When willow allocation for each parcel occurs based on the nearest potential plant location, transport costs are on average 23% lower than when all biomass is transported to the single Overpelt Fabriek site location. Therefore, when only considering transport costs, installing a smaller plant at each of the three potential plant locations would be less expensive than when installing a single biomass plant at the Overpelt Fabriek site. -- Highlights: ► Overpelt Fabriek site most attractive for time frames considered. ► Average tortuosity factor in Campine region between 1.27 and 1.42. ► Share of willow transport costs in willow supply costs 21%. ► Optimal allocation of willow results in lower transport costs

  3. Estonian energy forest project

    International Nuclear Information System (INIS)

    Koppel, A.; Kirt, E.; Kull, K.; Lasn, R.; Noormets, A.; Roostalu, H.; Ross, J.; Ross, V.; Sulev, M.

    1994-04-01

    In February 1993 an agreement of Swedish-Estonian scientific co-operation on energy forest was signed. In may five energy forest plantations (altogether 2 ha) were established in Estonia with Swedish selected clones of Salix viminalis and Salix dasyclados. The research within this project is carried out within three main directions. The studies of basic ecophysiological processes and radiation regime of willow canopy will be carried out in Toravere. The production ecology studies, comparison of the productivity of multiple clones on different soil types is based on the plantations as vegetation filter for wastewater purification is studied on the basis of plantations in Vaeike-Maarja and Valga (author)

  4. Yield and Water Quality Impacts of Field-Scale Integration of Willow into a Continuous Corn Rotation System.

    Science.gov (United States)

    Zumpf, Colleen; Ssegane, Herbert; Negri, Maria Cristina; Campbell, Patty; Cacho, Julian

    2017-07-01

    Agricultural landscape design has gained recognition by the international environmental and development community as a strategy to address multiple goals in land, water, and ecosystem service management; however, field research is needed to quantify impacts on specific local environments. The production of bioenergy crops in specific landscape positions within a grain-crop field can serve the dual purpose of producing cellulosic biomass (nutrient recovery) while also providing regulating ecosystem services to improve water quality (nutrient reduction). The effectiveness of such a landscape design was evaluated by the strategic placement of a 0.8-ha short-rotation shrub willow ( Seemen) bioenergy buffer along marginal soils in a 6.5-ha corn ( L.) field in a 6-yr field study in central Illinois. The impact of willow integration on water quality (soil water, shallow groundwater leaching, and crop nutrient uptake) and quantity (soil moisture and transpiration) was monitored in comparison with corn in the willow's first cycle of growth. Willows significantly reduced nitrate leachate in shallow subsurface water by 88% while maintaining adequate nutrient and water usage. Results suggest that willows offer an efficient nutrient-reduction strategy and may provide additional ecosystem services and benefits, including enhanced soil health. However, low values for calculated willow biomass will need to be readdressed in the future as harvest data become available to understand contributing factors that affected productivity beyond nutrient availability. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

  5. Modeling particle transport and discoloration risk in drinking water distribution networks

    Directory of Open Access Journals (Sweden)

    J. van Summeren

    2017-10-01

    Full Text Available Discoloration of drinking water is a worldwide phenomenon caused by accumulation and subsequent remobilization of particulate matter in drinking water distribution systems (DWDSs. It contributes a substantial fraction of customer complaints to water utilities. Accurate discoloration risk predictions could improve system operation by allowing for more effective programs on cleaning and prevention actions and field measurements, but are challenged by incomplete understanding on the origins and properties of particles and a complex and not fully understood interplay of processes in distribution networks. In this paper, we assess and describe relevant hydraulic processes that govern particle transport in turbulent pipe flow, including gravitational settling, bed-load transport, and particle entrainment into suspension. We assess which transport mechanisms are dominant for a range of bulk flow velocities, particle diameters, and particle mass densities, which includes common conditions for DWDSs in the Netherlands, the UK, and Australia. Our analysis shows that the theoretically predicted particle settling velocity and threshold shear stresses for incipient particle motion are in the same range as, but more variable than, previous estimates from lab experiments, field measurements, and modeling. The presented material will be used in the future development of a numerical modeling tool to determine and predict the spatial distribution of particulate material and discoloration risk in DWDSs. Our approach is aimed at understanding specific causalities and processes, which can complement data-driven approaches.

  6. Penicilllium discolor, a new species from cheese, nuts and vegetables

    DEFF Research Database (Denmark)

    Frisvad, Jens Christian; Samson, Robert A.; Rassing, Birgitte A.

    1997-01-01

    The new species Penicillum discolor, frequently isolated from nuts, vegetables and cheese is described. It is characterised by rough, dark green conidia, synnemateous growth on malt agar and the production of the secondary metabolites chaetoglobosins A, B and C, palitantin, cyclopenin, cyclopenol...

  7. Arthropods of native and exotic vegetation and their association with willow flycatchers and Wilson's warblers

    Science.gov (United States)

    Linda S. DeLay; Deborah M. Finch; Sandra Brantley; Richard Fagerlund; Michael D. Means; Jeffrey F. Kelly

    1999-01-01

    We compared abundance of migrating Willow Flycatchers and Wilson's Warblers to the abundance of arthropods in exotic and native vegetation at Bosque del Apache National Wildlife Refuge. We trapped arthropods using glue-boards in 1996 and 1997 in the same cottonwood, saltcedar, and willow habitats where we mist-netted birds during spring and fall migration. There...

  8. Surface discoloration of composite resins: Effects of staining and bleaching.

    Science.gov (United States)

    Poggio, Claudio; Beltrami, Riccardo; Scribante, Andrea; Colombo, Marco; Chiesa, Marco

    2012-09-01

    The purpose of this in vitro study was to evaluate surface discoloration of three microhybrid composite resins (Esthet•X HD, Clearfil AP-X, Gradia Direct) and five nanohybrid composite resins (Ceram•X, GC Kalore, G-aenial, Grandio, GrandioSO), after staining and bleaching procedures. The composite resins were polymerized with a curing light (Celalux II, Voco, Cuxhaven, Germany) into 160 silicon molds (6,4 mm in diameter and 2 mm in thickness) to obtain identical specimens. Twenty samples for each composite resin were prepared. The specimens were polished using an automated polishing machine with the sequence of 600-, 800-, 1000-grit abrasive paper under water irrigation. The specimens were immersed in tea and distilled water: the specimens were dipped for 20 min, once a day (every 24 h), for 14 days into the drinks. The specimens were then bleached with carbamide peroxide at 17% (Perfect Bleach-Voco). The color of specimens was measured with a spectrophotometer according to the CIE L(*)a(*)b(*) system after light-polymerization of composite resin specimens, after 7 days, after 14 days, and after bleaching. The color difference h index (DEab(*)) between each measurement was calculated. Statistical analysis was made using analysis of variance (ANOVA). All specimens showed a significant increase in staining with a similar trend and no significant differences between microhybrid and nanohybrid composite resins. After whitening procedures, materials tested showed both significant and unsignificant differences of the h index. Microhybrid and nanohybrid composite resins had similar in vitro surface discoloration in tea. After bleaching, discoloration was removed from some composite resins tested.

  9. Use of organic acids in acne and skin discolorations therapy

    Directory of Open Access Journals (Sweden)

    Alicja Kapuścińska

    2015-03-01

    Full Text Available Acne is one of the most frequent skin disorders that occurs in puberty, but often adults also have acne. The most important factors responsible for acne are elevated production of sebum by hyperactive sebaceous glands and blockage of the follicle because of hyperkeratosis [14]. The third etiopathogenic factor of acne is excessive microflora reproduction [8]. The most significant bacterium that is responsible for formation of skin lesions is Propionibacterium acnes, a rod-shaped Gram-positive and aerotolerant anaerobic bacterium. It is estimated that P. acnes is responsible for acne in approximately 80% of people aged 11 to 30 [27,40]. Even healed skin lesions can often cause skin discolorations and scar formation [51]. Exfoliating chemical substances that are commonly used in dermatology and cosmetology are organic acids. Exfoliating treatment using organic acids is called “chemical peeling” and consists of controlled application of those substances on the skin [38]. The depth of exfoliation depends on organic acid concentration, type of substance and contact time with the skin [41]. Using exfoliating agents seems to be helpful in excessive keratinization – one of several factors responsible for acne. Moreover, epidermis exfoliation is a popular method of removing skin discoloration [22]. Considering chemical structure, exfoliating substances that are most often used in cosmetology contain alpha-hydroxyacids (glycolic acid, lactic acid, mandelic acid and citric acid, beta-hydroxyacids (salicylic acid and other organic acids, such as trichloroacetic acid and pyruvic acid [47]. In this article, a literature review of use of organic acids in acne and skin discoloration therapy is presented.

  10. Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Rebeca García-Varela

    2015-10-01

    Full Text Available Traditional medicine has led to the discovery of important active substances used in several health-related areas. Phytochemicals in Rhoeo discolor extracts have proven to have important antimicrobial activity. In the present study, our group determined the antimicrobial effects of extracts of Rhoeo discolor, a plant commonly used in Mexico for both medicinal and ornamental purposes. We evaluated the in vitro activity of phenolic rich extracts against specifically chosen microorganisms of human health importance by measuring their susceptibility via agar-disc diffusion assay and flow cytometry: Gram-positive Listeria innocua and Streptococcus mutans, Gram-negative Escherichia coli and Pseudomonas aeruginosa, and lastly a fungal pathogen Candida albicans. Ten different extracts were tested in eight different doses on all the microorganisms. Analytical data revealed a high content of phenolic compounds. Both agar-disc diffusion assay and flow cytometry results demonstrated that Pseudomonas aeruginosa was the least affected by extract exposure. However, low doses of these extracts (predominantly polar, in a range from 1 to 4 μg/mL, did produce a statistically significant bacteriostatic and bactericidal effect on the rest of the microorganisms. These results suggest the addition of certain natural extracts from Rhoeo discolor could act as antibacterial and antimycotic drugs or additives for foods and cosmetics.

  11. Spectrophotometric analysis of discoloration and internal bleaching after use of different antibiotic pastes.

    Science.gov (United States)

    Fundaoğlu Küçükekenci, Funda; Çakici, Fatih; Küçükekenci, Ahmet Serkan

    2018-04-14

    To investigate teeth's antibiotic-induced color differences after bleaching using two different techniques. One hundred twenty extracted maxillar human incisors were examined. The specimens were randomly divided into six groups, each receiving one of six antibiotic paste fillings: (1) triple antibiotic paste (TAP) with minocycline, (2) double antibiotic paste (DAP), (3) TAP with amoxicillin, (4) TAP with cefaclor, (5) TAP with doxycycline, and (6) no filling (control group). Spectrophotometric measurements were obtained at baseline and then during the first, second, and third weeks after paste placement. The specimens discolored by antibiotics pastes were randomly divided into two subgroups: (1) internal bleaching with hydrogen peroxide (H 2 O 2) and (2) internal bleaching with H 2 O 2 plus Nd-YAG laser irradiation. The ∆E value was calculated and analyzed using a two-way analysis of variance and post-hoc Tukey's test (α = 0.05). The ∆E for all groups showed color differences exceeding the perceptibility threshold (∆E ˃ 3.7) at all time points except in the control and DAP groups. Minocycline-induced TAP showed the most severe coronal discoloration (32.42). When the ∆E was examined, thermo/photo bleaching (22.01 ± 8.23) caused more bleaching than walking bleaching (19.73 ± 5.73) at every time point (P = 0.19). No group returned to the original color after bleaching (P bleaching with Nd-YAG laser can be useful for bleaching/removing this discoloration. For clinically successful final appearances, understanding the effects of bleaching procedures on antibiotic paste discoloration is important.

  12. Environmental performance of gasified willow from different lands including land-use changes

    DEFF Research Database (Denmark)

    Saez de Bikuna Salinas, Koldo; Hauschild, Michael Zwicky; Pilegaard, Kim

    2017-01-01

    A life-cycle assessment (LCA) of a low-input, short rotation coppice (SRC) willow grown on different Danish lands was performed. Woodchips are gasified, producer gas is used for co-generation of heat and power (CHP) and the ash-char output is applied as soil amendment in the field. A hybrid model...... for abandoned farmland, as a relative C stock loss compared to natural regeneration. ILUC results show that area related GHG emissions are dominant (93% of iLUCfood and 80% of iLUCfeed), transformation being more important (82% of iLUCfood) than occupation (11%) impacts. LCA results show that CHP from willow...

  13. Regional Supplement to the Corps of Engineers Wetland Delineation Manual: Western Mountains, Valleys, and Coast Region (Version 2.0)

    Science.gov (United States)

    2010-05-01

    monophylla or P. edulis), junipers (Juniperus), cottonwoods (e.g., Populus fremontii), willows (Salix), or hardwoods (e.g., Quercus , Platanus...pine in association with incense cedar and California black oak ( Quercus kelloggii) on the western slopes and Jeffrey pine on the eastern slopes...corkbark fir (Abies lasiocarpa var. arizonica ), limber pine (Pinus flexilis), and bristlecone pine (P. aristata) (Bailey 1995). Black Hills (MLRA

  14. Discoloration of polyvinyl chloride (PVC) tape as a proxy for water-table depth in peatlands: validation and assessment of seasonal variability

    Science.gov (United States)

    Booth, Robert K.; Hotchkiss, Sara C.; Wilcox, Douglas A.

    2005-01-01

    Summary: 1. Discoloration of polyvinyl chloride (PVC) tape has been used in peatland ecological and hydrological studies as an inexpensive way to monitor changes in water-table depth and reducing conditions. 2. We investigated the relationship between depth of PVC tape discoloration and measured water-table depth at monthly time steps during the growing season within nine kettle peatlands of northern Wisconsin. Our specific objectives were to: (1) determine if PVC discoloration is an accurate method of inferring water-table depth in Sphagnum-dominated kettle peatlands of the region; (2) assess seasonal variability in the accuracy of the method; and (3) determine if systematic differences in accuracy occurred among microhabitats, PVC tape colour and peatlands. 3. Our results indicated that PVC tape discoloration can be used to describe gradients of water-table depth in kettle peatlands. However, accuracy differed among the peatlands studied, and was systematically biased in early spring and late summer/autumn. Regardless of the month when the tape was installed, the highest elevations of PVC tape discoloration showed the strongest correlation with midsummer (around July) water-table depth and average water-table depth during the growing season. 4. The PVC tape discoloration method should be used cautiously when precise estimates are needed of seasonal changes in the water-table.

  15. Ecophysiology of riparian cottonwood and willow before, during, and after two years of soil water removal.

    Science.gov (United States)

    Hultine, K R; Bush, S E; Ehleringer, J R

    2010-03-01

    Riparian cottonwood/willow forest assemblages are highly valued in the southwestern United States for their wildlife habitat, biodiversity, and watershed protection. Yet these forests are under considerable threat from climate change impacts on water resources and land-use activities to support human enterprise. Stream diversions, groundwater pumping, and extended drought have resulted in the decline of cottonwood/willow forests along many riparian corridors in the Southwest and, in many cases, the replacement of these forests with less desirable invasive shrubs and trees. Nevertheless, ecophysiological responses of cottonwood and willow, along with associated ecohydrological feedbacks of soil water depletion, are not well understood. Ecophysiological processes of mature Fremont cottonwood and coyote willow stands were examined over four consecutive growing seasons (2004-2007) near Salt Lake City, Utah, USA. The tree stands occurred near the inlet of a reservoir that was drained in the spring of 2005 and remained empty until mid-summer of 2006, effectively removing the primary water source for most of two growing seasons. Stem sap flux density (Js) in cottonwood was highly correlated with volumetric soil moisture (theta) in the upper 60 cm and decreased sevenfold as soil moisture dropped from 12% to 7% after the reservoir was drained. Conversely, Js in willow was marginally correlated with 0 and decreased by only 25% during the same period. Opposite patterns emerged during the following growing season: willow had a lower whole-plant conductance (kt) in June and higher leaf carbon isotope ratios (delta13C) than cottonwood in August, whereas k(t) and delta13C were otherwise similar between species. Water relations in both species recovered quickly from soil water depletion, with the exception that sapwood area to stem area (As:Ast) was significantly lower in both species after the 2007 growing season compared to 2004. Results suggest that cottonwood has a greater

  16. Some environmental impacts of short rotation willow coppice

    International Nuclear Information System (INIS)

    Slater, F.M.; Hodson, R.W.; Randrson, P.F.; Lynn, S.F.

    1997-01-01

    Short rotation willow coppice is a relatively new crop in upland Britain, and particularly in Environmentally Sensitive Areas the conservation and environmental effect of biomass crops needs to be evaluated. Investigations of sewage-sludge-treated plots in mid-Wales show that, because weed control was inadequate, recovery of the flora to its semi-natural precultivated state was rapid within and between experimental plots. Soil invertebrates responded to temporal stimuli before all else. Foliar-feeding invertebrates were greater in plots which had added fertilizer. Following cultivation voles were generally lost from the ploughed areas but field mice remained. Birds were studied in more extensive areas of short rotation coppice in central England and the assemblage of species was found to be similar to those found in conventional coppice but with a foreshortened successional sequence. The conservation value of short rotation willow coppice lies mainly in the abundant foliar invertebrates that provide a rich source of food for small passerine birds, particularly summer migrants. It also provides good cover for game birds - and their predators. (author)

  17. Characterization and reactions of a Salix extractive with a unique ring system

    Science.gov (United States)

    Lawrence Landucci; Sally Ralph; Kolby Hirth

    2003-01-01

    An extractive compound with a novel ring system was isolated from the wood of Salix alba and was given the name Salucci. The compound was primarily identified from the 1D NMR, 2D NMR and LC-MSMS data and the structure investigated through reactions and derivatizations.

  18. Determination of critical levels of residual oxygen to minimize discoloration of sliced packaged Norwegian salami under light display.

    Science.gov (United States)

    Sørheim, Oddvin; Måge, Ingrid; Larsen, Hanne

    2017-07-01

    Discoloration of sliced packaged salami is contributing to rejection of the product, food waste and economical loss. A combination of residual O 2 in the headspace of packages and light is causing photooxidation and deterioration of colour. The aim of this study was to establish maximum tolerable concentrations of residual O 2 in packages of salami slices with 100% N 2 under light display at 4 and 20°C. Salami sausages had variable inherent O 2 consumption rate. Storage of salami in 1% O 2 in darkness did not induce discoloration. The upper limits for O 2 for avoiding discoloration under light were variable in the range 0.1-1.0%, depending on temperature and type of salami. Display at 20°C increased the rate of O 2 depletion compared to 4°C. To minimize discoloration, sliced and packaged salami should be stored in darkness at approximately 20°C until the level of residual O 2 is reduced below a critical limit. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Study on radiation discoloration of PE and its mechanisms

    International Nuclear Information System (INIS)

    Wang Huiliang; Wang Chun; Chen Wenxiu

    2001-01-01

    In order to find the major reason for the radiation discoloration of PE, the changes of yellow index (YI) of HDPE and LDPE under γ irradiation in air or N 2 and after irradiation are measured using colorimeter. The growth and decay of alkyl free radical formed in HDPE and LDPE, and the change of the absorbance of conjugated double bonds formed in PE are measured using ESR and UV-Visible spectrometer. The YI of HDPE is higher than that of LDPE at the same radiation dose. The YI of PE irradiated in air is higher than that of PE irradiated in N 2 at the same radiation dose. The YI of PE irradiated different dose decreases with post-irradiation storage time firstly, up to a certain time, it becomes stable. It is found that the formation and decay of free radicals is very similar to that of the change of YI, whereas, the change of the absorbance of conjugated double bonds formed in PE is different to the change of YI. The results are discussed, it is believed that the major reason for the radiation discoloration is the formation of trapped free radicals

  20. Phytoscreening and phytoextraction of heavy metals at Danish polluted sites using willow and poplar trees.

    Science.gov (United States)

    Algreen, Mette; Trapp, Stefan; Rein, Arno

    2014-01-01

    The main purpose of this study was to determine typical concentrations of heavy metals (HM) in wood from willows and poplars, in order to test the feasibility of phytoscreening and phytoextraction of HM. Samples were taken from one strongly, one moderately, and one slightly polluted site and from three reference sites. Wood from both tree species had similar background concentrations at 0.5 mg kg(-1) for cadmium (Cd), 1.6 mg kg(-1) for copper (Cu), 0.3 mg kg(-1) for nickel (Ni), and 25 mg kg(-1) for zinc (Zn). Concentrations of chromium (Cr) and lead (Pb) were below or close to detection limit. Concentrations in wood from the highly polluted site were significantly elevated, compared to references, in particular for willow. The conclusion from these results is that tree coring could be used successfully to identify strongly heavy metal-polluted soil for Cd, Cu, Ni, Zn, and that willow trees were superior to poplars, except when screening for Ni. Phytoextraction of HMs was quantified from measured concentration in wood at the most polluted site. Extraction efficiencies were best for willows and Cd, but below 0.5% over 10 years, and below 1‰ in 10 years for all other HMs.

  1. Radiocaesium uptake and cycling in willow short rotation coppice

    International Nuclear Information System (INIS)

    Gommers, A.

    2002-01-01

    The document is an abstract of a PhD thesis. The study investigates the uptake and cycling of radiocaesium in willow short rotation coppice. Different factors influencing the soil-to-wood transfer of radiocaesium were investigated, among others the type of minerals, supply of potassium and soil composition

  2. Antioxidant and anti-inflammatory activities of porphyran isolated from discolored nori (Porphyra yezoensis).

    Science.gov (United States)

    Isaka, Shogo; Cho, Kichul; Nakazono, Satoru; Abu, Ryogo; Ueno, Mikinori; Kim, Daekyung; Oda, Tatsuya

    2015-03-01

    We found that discolored waste nori with no commercial value, contains much higher level of porphyran than normal nori that is a sheeted food stuff prepared from P. yezoensis used in sushi. Chemical analyses revealed that mean molecular mass of the porphyran prepared from discolored nori (dc-porphyran) was much lower than that of the porphyran from normal nori (n-porphyran). Dc-porphyran showed slightly greater scavenging activity toward superoxide anion and hydroxyl radical than n-porphyran. Dc-porphyran inhibited nitric oxide (NO) production in LPS-stimulated RAW264.7 cells through preventing the expression of inducible NO synthase, whereas no such activity was observed in n-porphyran. Since acid-hydrolyzed n-porphyran showed the inhibitory activity on NO production from LPS-stimulated RAW264.7 cells, the molecular size of porphyran was suggested to be a critical factor for the activity. Dc-porphyran was separated into 4 fractions (F1-F4) on DEAE-chromatography, and F1 showed the highest inhibitory effect on NO production from LPS-stimulated RAW264.7 cells. Our results indicate that discolored waste nori is useful as a source of porphyran with even better bioactivities than porphyran from normal nori. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Habitat and sex differences in physiological condition of breeding Southwestern Willow Flycatchers (Empidonax traillii extimus)

    Science.gov (United States)

    Owen, J.C.; Sogge, M.K.; Kern, M.D.

    2005-01-01

    The Southwestern Willow Flycatcher (Empidonax traillii extimus; here- after “flycatcher”) is a federally listed endangered species that breeds in densely vegetated riparian habitats dominated by native and exotic plants, including introduced monotypic saltcedar (Tamarix ramosissima). Some workers have theorized that saltcedar is unsuitable habitat for the flycatcher, primarily because it generally supports a smaller and less diverse invertebrate community (the flycatcher's food base) than native habitats (e.g. Salix spp.). However, differences in insect communities between native and saltcedar habitats are not proof that saltcedar habitats are inferior. The only way to evaluate whether the habitats differ in dietary or energetic quality is to document actual food limitation or its manifestations. Measurements of an individual's body condition and metabolic state can serve as indicators of environmental stressors, such as food limitation and environmental extremes. We captured 130 flycatchers breeding in native and saltcedar habitats in Arizona and New Mexico and measured 12 variables of physiological condition. These variables included body mass, fat level, body condition index, hematocrit, plasma triglycerides, plasma free fatty acids and glycerol, plasma glucose and beta-hydroxybutyrate, plasma uric acid, total leukocyte count, and heterophil-to-lymphocyte ratio. We found substantial sex-based differences in the condition of male and female flycatchers. Ten of the 12 measures of physiological condition differed significantly between the sexes. In all cases where male and female condition differed (except mass), the differences suggest that males were in poorer condition than females. We found few habitat-based differences in flycatcher condition. Only 3 of the 12 physiological condition indices differed significantly between habitats. Our data show that, at least in some parts of the flycatcher's range, there is no evidence that flycatchers breeding in

  4. Spatial distribution of arsenic and heavy metals in willow roots from a contaminated floodplain soil measured by X-ray fluorescence spectroscopy

    International Nuclear Information System (INIS)

    Zimmer, Dana; Kruse, Jens; Baum, Christel; Borca, Camelia; Laue, Michael; Hause, Gerd; Meissner, Ralph; Leinweber, Peter

    2011-01-01

    Under changing redox conditions some plants create plaques at their root surface, which may affect the mobility and uptake of As and heavy metals but it is unknown to what extent this also holds true for willows in contaminated floodplain soils. Therefore, willow roots were sampled from a phytoremediation trial in the contaminated floodplain of the river Elbe (Germany), cryofixed, freeze-dried, and cross sections were mapped for the distribution of As, Ca, Cu, Fe, K, Mn, Ni, S and Zn by synchrotron based X-ray fluorescence spectroscopy. The elements Ca, Cu, Ni, S and Zn were concentrated in the aerenchymatic tissue, and not associated with Fe and Mn. Mixed Fe-Mn plaques covered the surface of the willow roots and As was accumulated in these plaques. The observed association pattern between As and Fe was explained by the different sorption/desorption properties of As(III) and As(V). The Cu and Zn intensities were not associated with the intensity of Fe in the plaque, which seems to be a willow-specific difference compared to other wetland plants. These results suggested that willows are especially suited to stabilize low-phytoextractable elements like Cu and As in their roots and rhizosphere. Thus, short rotation coppicing of willows may be a practical approach to mitigate the adverse effects of floodplain soil contamination. - Research highlights: → Elemental distributions were mapped on willow roots for the first time by synchrotron-based X-ray fluorescence. → Ca, Cu, Ni, S and Zn were enriched in the aerenchyma but As, Fe and Mn formed root plaques. → The Cu and Zn enrichments in aerenchyma but absence in plaques appeared to be willow-specific. → In the plaques were three groups of pixels which strongly differed in the As to Fe and As to Mn ratios. → This indicated different species of these redox-sensitive elements.

  5. Spatial distribution of arsenic and heavy metals in willow roots from a contaminated floodplain soil measured by X-ray fluorescence spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Zimmer, Dana, E-mail: dana.zimmer@uni-rostock.de [Soil Science, University of Rostock, Justus-von-Liebig-Weg 6, D-18051 Rostock (Germany); Kruse, Jens; Baum, Christel [Soil Science, University of Rostock, Justus-von-Liebig-Weg 6, D-18051 Rostock (Germany); Borca, Camelia [Paul Scherrer Institute, Swiss Light Source, CH-5232 Villigen PSI (Switzerland); Laue, Michael [Electron Microscopy Centre, University of Rostock, Medical Faculty, Strempelstr. 14, D-18057 Rostock (Germany); Hause, Gerd [Microscopy Unit, Biocenter of the University of Halle, Weinbergweg 22, D-06120 Halle/Saale (Germany); Meissner, Ralph [UFZ-Helmholtz Centre for Environmental Research, Department of Soil Physics, Lysimeter Station, Dorfstrasse 55, D-39615 Falkenberg (Germany); Leinweber, Peter [Soil Science, University of Rostock, Justus-von-Liebig-Weg 6, D-18051 Rostock (Germany)

    2011-09-01

    Under changing redox conditions some plants create plaques at their root surface, which may affect the mobility and uptake of As and heavy metals but it is unknown to what extent this also holds true for willows in contaminated floodplain soils. Therefore, willow roots were sampled from a phytoremediation trial in the contaminated floodplain of the river Elbe (Germany), cryofixed, freeze-dried, and cross sections were mapped for the distribution of As, Ca, Cu, Fe, K, Mn, Ni, S and Zn by synchrotron based X-ray fluorescence spectroscopy. The elements Ca, Cu, Ni, S and Zn were concentrated in the aerenchymatic tissue, and not associated with Fe and Mn. Mixed Fe-Mn plaques covered the surface of the willow roots and As was accumulated in these plaques. The observed association pattern between As and Fe was explained by the different sorption/desorption properties of As(III) and As(V). The Cu and Zn intensities were not associated with the intensity of Fe in the plaque, which seems to be a willow-specific difference compared to other wetland plants. These results suggested that willows are especially suited to stabilize low-phytoextractable elements like Cu and As in their roots and rhizosphere. Thus, short rotation coppicing of willows may be a practical approach to mitigate the adverse effects of floodplain soil contamination. - Research highlights: {yields} Elemental distributions were mapped on willow roots for the first time by synchrotron-based X-ray fluorescence. {yields} Ca, Cu, Ni, S and Zn were enriched in the aerenchyma but As, Fe and Mn formed root plaques. {yields} The Cu and Zn enrichments in aerenchyma but absence in plaques appeared to be willow-specific. {yields} In the plaques were three groups of pixels which strongly differed in the As to Fe and As to Mn ratios. {yields} This indicated different species of these redox-sensitive elements.

  6. Differential impacts of willow and mineral fertilizer on bacterial communities and biodegradation in diesel fuel oil-contaminated soil

    Directory of Open Access Journals (Sweden)

    Mary-Cathrine C.E. Leewis

    2016-06-01

    Full Text Available Despite decades of research there is limited understanding of how vegetation impacts the ability of microbial communities to process organic contaminants in soil. Using a combination of traditional and molecular assays, we examined how phytoremediation with willow and/or fertilization affected the microbial community present and active in the transformation of diesel contaminants. In a pot study, willow had a significant role in structuring the total bacterial community and resulted in significant decreases in diesel range organics (DRO. However, stable isotope probing (SIP indicated that fertilizer drove the differences seen in community structure and function. Finally, analysis of the total variance in both pot and SIP experiments indicated an interactive effect between willow and fertilizer on the bacterial communities. This study clearly demonstrates that a willow native to Alaska accelerates DRO degradation, and together with fertilizer, increased aromatic degradation by shifting microbial community structure and the identity of active naphthalene degraders.

  7. Buffalo Harbor Study. Preliminary Feasibility Report. Volume I. Main Report.

    Science.gov (United States)

    1983-04-01

    to usually narrow strips of riparian vegetation, which is composed of various trees and shrubs of the Salix genus (willow), sumac, aspen, boxelder...reptiles were found. Species included, leopard frogs, snapping turtles, painted turtle, and garter snakes (SUNY Brockport: 1982). (7) Endangered Species...vessel traffic on the Buffalo River causes interrupted truck service. Firms have also cited snow removal as a problem. The harbor area road service

  8. Central Radar System, Over-the-Horizon Backscatter

    Science.gov (United States)

    1990-03-09

    Magnolia virginiana Switchgrass Panicum virgatum FAC+ Tall mannagrass Glyceria grandis OBL Water fern Salvinia spp. and Marsilea vestita OBL Water...boxelder, (Acer negundo), oaks (Quercus spp.), willows (Salix spp.), chokecherry (Prunus virginiana ), plum (Prunus spp.), and russian olive (Elaeagnus...maintenance of the power lines may be required to trim or prune trees which may interfere with the lines. With the exception of the pole sites, the only

  9. Sterling C. Robertson Dam and Limestone Lake on the Navasota River, Texas (Leon, Limestone and Robertson Counties).

    Science.gov (United States)

    1976-04-01

    flatsedge (Cyperus s p.) , and Spanish mulberry (Callicarpa americana). Common bottomland forest species included pecan ( Carya illinoensis ), post...ona-wiibT Bull Briar Smilax glauca Cat Greenbriar Salix nigra Black Willow Carya illinoensis Pecan Carya texana Black Hickory Q-erustlefata Post Oak... illinoensis ), post oak (Quercus stellata). hackberry (Ce/is sp.), elm (Ulmus sp.), and holly. Species common in the prairie site included Croton sp., prairie

  10. Vegetation Resources of Rocky Mountain Arsenal, Adams County, Colorado

    Science.gov (United States)

    1989-10-01

    SUCCULENTS Opuntia polyacantha Plains Prickly Pear Cactaceae Table 4 .(cont’d.) Scientific Name Common Name Family Name Yucca glauca Spanish Bayonet Agavaceae...Lycium halimitolium Matrim~ony Bush Solanceae Salix exigua Coyote Willow Salicaceae CACTI AND SUCCULENTS Coryphantha vivipara Ball Cactus Cactaceae ...Qpuntia compressa Prickly Pear Cactus Cactaceae Opuntia polyacantha Plains Prickly Pear Cactaceae Yucca glauca Spanish Bayonet Agavaceae cq Nl Nl Co~ V c0

  11. ent-Pimarane and ent-Kaurane Diterpenes from Aldama discolor (Asteraceae and Their Antiprotozoal Activity

    Directory of Open Access Journals (Sweden)

    Mauro S. Nogueira

    2016-09-01

    Full Text Available Aldama discolor (syn.Viguiera discolor is an endemic Asteraceae from the Brazilian “Cerrado”, which has not previously been investigated for its chemical constituents and biological activity. Diterpenes are common secondary metabolites found in Aldama species, some of which have been reported to present potential antiprotozoal and antimicrobial activities. In this study, the known ent-3-α-hydroxy-kaur-16-en-18-ol (1, as well as three new diterpenes, namely, ent-7-oxo-pimara-8,15-diene-18-ol (2, ent-2S,4S-2-19-epoxy-pimara-8(3,15-diene-7β-ol (3 and ent-7-oxo-pimara-8,15-diene-3β-ol (4, were isolated from the dichloromethane extract of A. discolor leaves and identified by means of MS and NMR. The compounds were assayed in vitro against Trypanosoma brucei rhodesiense, T. cruzi and Leishmania donovani, Plasmodium falciparum and also tested for cytotoxicity against mammalian cells (L6 cell line. The ent-kaurane 1 showed significant in vitro activity against both P. falciparum (IC 50 = 3.5 μ M and L. donovani (IC 50 = 2.5 μ M and ent-pimarane 2 against P. falciparum (IC 50 = 3.8 μ M. Both compounds returned high selectivity indices (SI >10 in comparison with L6 cells, which makes them interesting candidates for in vivo tests. In addition to the diterpenes, the sesquiterpene lactone budlein A (5, which has been reported to possess a strong anti-T. b. rhodesiense activity, was identified as major compound in the A. discolor extract and explains its high activity against this parasite (100% growth inhibition at 2 μ g/mL.

  12. Capitate glandular trichomes in Aldama discolor (Heliantheae - Asteraceae): morphology, metabolite profile and sesquiterpene biosynthesis.

    Science.gov (United States)

    Bombo, A B; Appezzato-da-Glória, B; Aschenbrenner, A-K; Spring, O

    2016-05-01

    The capitate glandular trichome is the most common type described in Asteraceae species. It is known for its ability to produce various plant metabolites of ecological and economic importance, among which sesquiterpene lactones are predominant. In this paper, we applied microscopy, phytochemical and molecular genetics techniques to characterise the capitate glandular trichome in Aldama discolor, a native Brazilian species of Asteraceae, with pharmacological potential. It was found that formation of trichomes on leaf primordia of germinating seeds starts between 24 h and 48 h after radicle growth indicates germination. The start of metabolic activity of trichomes was indicated by separation of the cuticle from the cell wall of secretory cells at the trichome tip after 72 h. This coincided with the accumulation of budlein A, the major sesquiterpene lactone of A. discolor capitate glandular trichomes, in extracts of leaf primordia after 96 h. In the same timeframe of 72-96 h post-germination, gene expression studies showed up-regulation of the putative germacrene A synthase (pGAS2) and putative germacrene A oxidase (pGAO) of A. discolor in the transcriptome of these samples, indicating the start of sesquiterpene lactone biosynthesis. Sequencing of the two genes revealed high similarity to HaGAS and HaGAO from sunflower, which shows that key steps of this pathway are highly conserved. The processes of trichome differentiation, metabolic activity and genetic regulation in A. discolor and in sunflower appear to be typical for other species of the subtribe Helianthinae. © 2015 German Botanical Society and The Royal Botanical Society of the Netherlands.

  13. ent-Pimarane and ent-Kaurane Diterpenes from Aldama discolor (Asteraceae) and Their Antiprotozoal Activity.

    Science.gov (United States)

    Nogueira, Mauro S; Da Costa, Fernando B; Brun, Reto; Kaiser, Marcel; Schmidt, Thomas J

    2016-09-15

    Aldama discolor (syn.Viguiera discolor) is an endemic Asteraceae from the Brazilian "Cerrado", which has not previously been investigated for its chemical constituents and biological activity. Diterpenes are common secondary metabolites found in Aldama species, some of which have been reported to present potential antiprotozoal and antimicrobial activities. In this study, the known ent-3-α-hydroxy-kaur-16-en-18-ol (1), as well as three new diterpenes, namely, ent-7-oxo-pimara-8,15-diene-18-ol (2), ent-2S,4S-2-19-epoxy-pimara-8(3),15-diene-7β-ol (3) and ent-7-oxo-pimara-8,15-diene-3β-ol (4), were isolated from the dichloromethane extract of A. discolor leaves and identified by means of MS and NMR. The compounds were assayed in vitro against Trypanosoma brucei rhodesiense, T. cruzi and Leishmania donovani, Plasmodium falciparum and also tested for cytotoxicity against mammalian cells (L6 cell line). The ent-kaurane 1 showed significant in vitro activity against both P. falciparum (IC 50 = 3.5 μ M) and L. donovani (IC 50 = 2.5 μ M) and ent-pimarane 2 against P. falciparum (IC 50 = 3.8 μ M). Both compounds returned high selectivity indices (SI >10) in comparison with L6 cells, which makes them interesting candidates for in vivo tests. In addition to the diterpenes, the sesquiterpene lactone budlein A (5), which has been reported to possess a strong anti-T. b. rhodesiense activity, was identified as major compound in the A. discolor extract and explains its high activity against this parasite (100% growth inhibition at 2 μ g/mL).

  14. Woody plant willow in function of river water protection

    Directory of Open Access Journals (Sweden)

    Babincev Ljiljana M.

    2011-01-01

    Full Text Available Coastal area surrounding the river Ibar, in the area between cities of Kosovska Mitrovica and Leposavić in the north of Kosovo and Metohija, is occupied with seven industrial waste dumps. These dumps were all part of the exploitation and flotation refinement of raw mineral materials, metallurgic refinement of concentrates, chemical industry, industrial refinement and energetic facilities of Trepča industrial complex. The existing waste dumps, both active and inactive, are of heterogenic chemical composition. Its impact on the river water is shown by the content of heavy metals found in it. Removal of lead, cadmium and zinc would be economically unrewarding, regardless of the technology used. Wooden plant that prevails in this area is white willow. This work is focused on the removal of heavy metals (Pb, Cd and Zn from the water of the river Ibar using white willow. Roots of the willow are cultivated using the method of water cultures in an individual solution of heavy metals and river water sample. The preparation of the samples for analysis was performed by burning the herbal material and dissolving ashes in the appropriate acids. The concentrations of metals were determined by the stripping analysis. In the investigated heavy metal solutions the biomass increase is 25.6% in lead solution, 27.3% in cadmium and 30.7% in zinc solution. The increase of biomass in nutritional solution, without the heavy metals, is 32.4% and in river water sample 27.5%. The coefficient of bioaccumulation in solutions with heavy metals is 1.6% in lead solution, 1.9% in cadmium and 2.2% in zinc solution. Heavy metals accumulation is 18.74 μg of lead, 20.09 μg of cadmium and 22.89 μg of zinc. The coefficient of bioaccumulation of the water samples, that contained 44.83 μg/dm3 of lead, 29.21 μg/dm3 of cadmium and 434.00 μg/dm3 of zinc, during the period of 45 days, was 30.3% for lead, 53.4% for cadmium and 3.9% for zinc. The concentrations of accumulated metals

  15. The Effect of Methylation and Anti-Oxidant on Discoloration of Weathered Wood Plastic Composites

    Directory of Open Access Journals (Sweden)

    Peivand Darabi

    2011-01-01

    Full Text Available As the outdoor application of Wood Plastic Composites (WPCs become more widespread, the resistance of these products against weathering, particularly ultraviolet (UV light becomes more important. When WPCs are exposed to outdoor ultraviolet light, rain, snow and atmosphere pollution, they will be degraded which can be indicated by color fade. To investigate the effects of methylation and Anti-Oxidant separately and together on discoloration of weathered wood plastic composites, composites of poplar wood flour and high density polyethylene.Were made according to the ASTMD 2565, samples were placed in Atlas Xenon apparatus for 250 and 2000 hours. Discoloration and FT-IR spectra of the samples were measured and compared. The results have shown that methylation in short term and long term can relatively reduce the discoloration of weathered samples and also in short term can hinder the photodegradation. FT-IR spectra showed that, in long term, neither of the treatments could protect lignin from irradiation within wood flour. But methylation limited the depth of penetration of weathering. The Antioxidant did not have an influence on color change in a long period of time, but was able to relatively decrease it in short term.

  16. The Development of improved willow clones for eastern North America

    Science.gov (United States)

    R. F. Kopp; L. B. Smart; C. A. Maynard; J. G. Isebrands; G. A. Tuskan; L. P. Abrahamson

    2001-01-01

    Efforts aimed at genetic improvement of Salix are increasing in North America.Most of these are directed towards developing improved clones for biomass production, phytoremediation, nutrient filters, and stream bank stabilization in the Northeast and North-central United States. Native species are of primary interest, but a small number of clones containing non-native...

  17. Accumulation of Elements in Salix and Other Species Used in Vegetation Filters with Focus on Wood Fuel Quality

    Energy Technology Data Exchange (ETDEWEB)

    Adler, Anneli

    2007-07-01

    Woody or herbaceous perennials used as vegetation filters for treatment of different types of wastes can be suitable for production of solid biofuels when their above ground harvestable biomass yield is sufficiently high and when biomass contains appropriate concentrations of minerals with regard to fuel combustion processes. The concentrations of nitrogen (N), potassium (K) and heavy metals (especially Zn and Cd) in fuel should be low and calcium (Ca) concentrations high to avoid technical problems and environmentally harmful emissions during combustion. Since soil supplementation with essential elements improves biomass yield, a conflict might arise between yield and quality aims. There are various possibilities to influence fuel quality during the growing phase of the life cycle of perennial biomass crops. This study assessed the suitability of two deciduous woody perennials (Salix and Populus) and two summer green herbaceous perennials (Phragmites and Urtica) for phytoremediation in terms of growth and nutrient allocation patterns. Salix and Populus proved suitable as vegetation filters when nutrients were available to plants in near-optimal proportions, but when unbalanced nutrient solutions (wastewater) were applied, stem biomass fraction was strongly reduced. Phragmites was more tolerant to wastewater treatment in terms of plant biomass production and nutrient allocation patterns, so if the N:P ratio of the wastewater is suboptimal, a vegetation filter using Phragmites could be considered. In further studies, a method was developed to determine the proportions of nutrient-rich bark in coppiced Salix, while heavy metal phytoextraction capacity was assessed in two Salix vegetation filters. The relevance of proportion of bark on wood fuel quality and element removal from vegetation filters was also investigated. The concentrations of the elements studied in harvestable Salix shoot biomass were higher, meaning lower wood fuel quality, in plantations where

  18. Above- and Belowground Development of a Fast-Growing Willow Planted in Acid-Generating Mine Technosol.

    Science.gov (United States)

    Guittonny-Larchevêque, M; Lortie, S

    2017-11-01

    Surface metal mining produces large volumes of waste rocks. If they contain sulfide minerals, these rocks can generate a flow of acidic water from the mining site, known as acid mine drainage (AMD), which increases trace metals availability for plant roots. Adequate root development is crucial to decreasing planting stress and improving phytoremediation with woody species. However, techniques to improve revegetation success rarely take into account root development. An experiment was conducted at a gold mine in Quebec, Canada, to evaluate the establishment ability over 3 yr of a fast-growing willow ( Sx64) planted in acid-generating waste rocks. The main objective was to study root development in the soil profile and trace element accumulation in leaves among substrates varying in thickness (0, 20, and 40 cm of soil) and composition (organic carbon [OC] and alkaline AMD treatment sludge). Trees directly planted in waste rocks survived well (69%) but had the lowest productivity (lowest growth in height and diameter, aerial biomass, total leaf area, and root-system size). By contrast, the treatment richer in OC showed the greatest aerial biomass and total leaf area the first year; the thicker treatment resulted in the greatest growth in height and diameter, aboveground biomass, and root-system size in both the first and third years. Willow root development was restricted to soil layers during the first year, but this restriction was overcome in the third year after planting. Willow accumulation factors in leaves were below one for all investigated trace metals except for zinc (Zn), cadmium (Cd), and strontium. For Cd and Zn, concentrations increased with time in willow foliage, decreasing the potential of this willow species use for phytostabilization, despite its ability to rapidly develop extensive root systems in the mine Technosol. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

  19. Optimization of Paper Discoloration via Pyrolysis Using Lasers

    KAUST Repository

    Alhashem, Mayadah M.

    2017-01-01

    drying, devolatilization (pyrolysis, or distillation phase), and lastly, the char (charcoal) combustion. These stages are driven by heat from the CO2 laser. Moving the laser rapidly above the paper surface arrests the reaction at the second stage, after the formation of blackened char. The control variables in the experimental method are laser power, speed, and the vertical position that affects the laser intensity. Computer software controls these variables. The discoloration of paper is quantified by measuring the light absorptivity using a UV-Vis-IR Spectrometer.

  20. Spatial distribution of arsenic and heavy metals in willow roots from a contaminated floodplain soil measured by X-ray fluorescence spectroscopy.

    Science.gov (United States)

    Zimmer, Dana; Kruse, Jens; Baum, Christel; Borca, Camelia; Laue, Michael; Hause, Gerd; Meissner, Ralph; Leinweber, Peter

    2011-09-01

    Under changing redox conditions some plants create plaques at their root surface, which may affect the mobility and uptake of As and heavy metals but it is unknown to what extent this also holds true for willows in contaminated floodplain soils. Therefore, willow roots were sampled from a phytoremediation trial in the contaminated floodplain of the river Elbe (Germany), cryofixed, freeze-dried, and cross sections were mapped for the distribution of As, Ca, Cu, Fe, K, Mn, Ni, S and Zn by synchrotron based X-ray fluorescence spectroscopy. The elements Ca, Cu, Ni, S and Zn were concentrated in the aerenchymatic tissue, and not associated with Fe and Mn. Mixed Fe-Mn plaques covered the surface of the willow roots and As was accumulated in these plaques. The observed association pattern between As and Fe was explained by the different sorption/desorption properties of As(III) and As(V). The Cu and Zn intensities were not associated with the intensity of Fe in the plaque, which seems to be a willow-specific difference compared to other wetland plants. These results suggested that willows are especially suited to stabilize low-phytoextractable elements like Cu and As in their roots and rhizosphere. Thus, short rotation coppicing of willows may be a practical approach to mitigate the adverse effects of floodplain soil contamination. Copyright © 2011 Elsevier B.V. All rights reserved.

  1. Cadmium concentrations in tissues of willow ptarmigan (Lagopus lagopus) and rock ptarmigan (Lagopus muta) in Nunavik, Northern Quebec

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigue, Jean [Environment Canada, Service canadien de la faune, 1141 route de l' Eglise, Sainte-Foy, Quebec, G1V 4H5 (Canada)]. E-mail: jean.rodrigue@ec.gc.ca; Champoux, Louise [Environment Canada, Service canadien de la faune, 1141 route de l' Eglise, Sainte-Foy, Quebec, G1V 4H5 (Canada); Leclair, Daniel [Centre de recherche du Nunavik, Societe Makivik, C.P. 179, Kuujjuaq, Quebec, J0M 1C0 (Canada); Duchesne, Jean-Francois [Unite de recherche en sante publique du CHUQ, 945, avenue Wolfe, Sainte-Foy, Quebec, G1V 5B3 (Canada)

    2007-06-15

    Willow and rock ptarmigan were obtained from Northern Quebec. Willow ptarmigan were found to have mean cadmium concentrations of 179.7 {mu}g/g (dw) in the kidneys and 25.8 {mu}g/g (dw) in the liver; these levels were three times higher than those found in the rock ptarmigan. The cadmium levels in the ptarmigan were below the threshold above which adverse effects can be observed in birds. The difference between the two ptarmigan species in cadmium content is explained by the diet. A comparison of their diet showed that willow, which stores cadmium, is an important food resource for willow ptarmigan but not for rock ptarmigan. Because there is limited information available on the consumption of ptarmigan kidneys and liver by the Inuit, and the fact that this is a traditional way of life and provides nutritional benefits to the Inuit population, no consumption guidelines are proposed. - High levels of cadmium were found in ptarmigan in Northern Quebec. No consumption guidelines are proposed for the Inuit people.

  2. Cadmium concentrations in tissues of willow ptarmigan (Lagopus lagopus) and rock ptarmigan (Lagopus muta) in Nunavik, Northern Quebec

    International Nuclear Information System (INIS)

    Rodrigue, Jean; Champoux, Louise; Leclair, Daniel; Duchesne, Jean-Francois

    2007-01-01

    Willow and rock ptarmigan were obtained from Northern Quebec. Willow ptarmigan were found to have mean cadmium concentrations of 179.7 μg/g (dw) in the kidneys and 25.8 μg/g (dw) in the liver; these levels were three times higher than those found in the rock ptarmigan. The cadmium levels in the ptarmigan were below the threshold above which adverse effects can be observed in birds. The difference between the two ptarmigan species in cadmium content is explained by the diet. A comparison of their diet showed that willow, which stores cadmium, is an important food resource for willow ptarmigan but not for rock ptarmigan. Because there is limited information available on the consumption of ptarmigan kidneys and liver by the Inuit, and the fact that this is a traditional way of life and provides nutritional benefits to the Inuit population, no consumption guidelines are proposed. - High levels of cadmium were found in ptarmigan in Northern Quebec. No consumption guidelines are proposed for the Inuit people

  3. Isolation of Secondary Metabolites from the Roots of Salix Babylonica

    Science.gov (United States)

    Singh, Harpreet; Raturi, Rakesh; Badoni, P. P.

    2017-08-01

    Chemical examination of ethanolic extract of Salix babylonica, root, led to isolation of 2-(Hydroxymethyl) phenyl β-D-glucopyranoside(1) and 2-[(benzoyloxy)methyl]-4-hydroxyphenyl β-D-glucopyranoside 3-Benzoate (2)with previously known compounds β-Sitosterol, kaempferol 7-O-glucoside andapigenin 7-O-galactoside. Compounds 1and 2 had been isolated for the first time from this plant. The structures of the compounds had been elucidated with the help of spectral and chemical studies.

  4. A New Flavonoid Glycoside from Salix denticulata Aerial Parts

    Directory of Open Access Journals (Sweden)

    Amita Bamola

    2009-09-01

    Full Text Available Abstract: A new flavonoid glycoside (1 has been isolated from the aerial parts of Salix denticulata (Salicaceae together with five known compounds, β-sitosterol, 2,6-dihydroxy- 4-methoxy acetophenone, eugenol-1-O-β-D-glucopyranoside, 1-O-β-D-(3’-benzoyl salicyl alcohol and luteolin-7-O-β-D-glucopyranosyl-(1-6-glucopyranoside. The structure of 1 was elucidated as 2’,5-dihydroxy-3’-methoxyflavone-7-O-β-D-glucopyranoside by means of chemical and spectral data including 2D NMR studies.

  5. Biomass power for rural development. Technical progress report, Phase 2, July 1--September 30, 1998

    Energy Technology Data Exchange (ETDEWEB)

    Neuhauser, E.

    1999-01-01

    The project undertaken by the Salix Consortium is a multi-phased, multi-partner endeavor. Phase 1 focused on initial development and testing of the technology and forging the necessary agreements to demonstrate commercial willow production. The Phase 1 objectives have been successfully completed: preparing final design plans for two utility pulverized coal boiler for 20 MW of biopower capacity; developing fuel supply plans for the project with a goal of establishing 365 ha (900 ac) of willow; obtaining power production commitments from the power companies for Phase 2; obtaining construction and environmental permits; and developing an experimental strategy for crop production and power generation improvements needed to assure commercial success. The R and D effort also addresses environmental issues pertaining to introduction of the willow energy system. Beyond those Phase 1 requirements, the Consortium has already successfully demonstrated cofiring at Greenidge Station and has initiated development of the required nursery capacity for acreage scale-up. In Phase 2 every aspect of willow production and power generation from willow biomass will be demonstrated. The ultimate objective of Phase 2 is to transition the work performed under the Biomass Power for Rural Development project into a thriving, self-supported energy crop enterprise.

  6. With willows and poplars against the heavy metals

    International Nuclear Information System (INIS)

    Jaros, M.

    2002-01-01

    Special kinds of trees take out cadmium, lead and zinc from the soil, helping its rehabilitation in contaminated regions. A new method for soil rehabilitation (phytoremediation) based on plants is described. Special willows and poplars have the capability to extract as a sponge heavy metals from the soils and store them in their leaves. For example, they can uptake 700 times the amount of cadmium than the normal plants do. (nevyjel)

  7. Recreational Appendix Report, Elm Fork Flood Control Project, Dallas and Denton Counties, Texas.

    Science.gov (United States)

    1973-05-01

    Juniperus virginiana 2. Willow Salix nigra 3. Cottonwood Populus deltoides 4. Black Walnut Juglans migra 5. Pecan Carya illinoensis 6. Bur Oak...Maclura pomifera 12. Red Mulberry Morus rubra 13. Sycamore Platanus occidentailis 14. Red Haw Crataegus, sps. 15. Wild Plum Prunus mexicana 16. Mesquite...also provides a habitat for a number of mammals including: 1. Opossum Didelphis virginiana 2. Shrews Blarina brevicuada and Cryptotis parva 3. Raccoon

  8. Threatened Vertebrate Species Occurring or Believed to Occur in the Floodplains of the Mississippi River between Cairo, Illinois, and Minneapolis, Minnesota, and of the Illinois Waterway between Grafton, Illinois, and Chicago, Illinois,

    Science.gov (United States)

    1975-01-01

    heterophylla), and black willow (Salix nigra), with swamp white oak ( Quercus bicolor) and pin oak (p. valustri3) also important. Skorepa and Ozment (1968...of its original prairie habitat has seen destroyed. GREAT PLAINS RAT SNAKE Elaphe guttata emoryi (Baird and Girard) (Ill. -Rare) The Great Plains Rat...forficatus 119 F Clemmys guttata 41, 117 Elaphe guttata emoryi 49, 118 Cnemidophorus sexlineatus 43, 44, 117 Elassoma zonatum. 27, 114 Colinus virginianus

  9. Environmental life cycle assessment of producing willow, alfalfa and straw from spring barley as feedstocks for bioenergy or biorefinery systems

    DEFF Research Database (Denmark)

    Parajuli, Ranjan; Knudsen, Marie Trydeman; Djomo, Sylvestre Njakou

    2017-01-01

    The current study aimed at evaluating potential environmental impacts for the production of willow, alfalfa and straw from spring barley as feedstocks for bioenergy or biorefinery systems. A method of Life Cycle Assessment was used to evaluate based on the following impact categories: Global...... and land occupation. Environmental impacts for straw were economically allocated from the impacts obtained for spring barley. The results obtained per ton dry matter showed a lower carbon footprint for willow and alfalfa compared to straw. It was due to higher soil carbon sequestration and lower N2O...... emissions. Likewise, willow and alfalfa had lower EP than straw. Straw had lowest NRE use compared to other biomasses. PFWTox was lower in willow and alfalfa compared to straw. A critical negative effect on soil quality was found with the spring barley production and hence for straw. Based on the energy...

  10. Are winter-active species vulnerable to climate warming? A case study with the wintergreen terrestrial orchid, Tipularia discolor.

    Science.gov (United States)

    Marchin, Renée M; Dunn, Robert R; Hoffmann, William A

    2014-12-01

    In the eastern United States, winter temperature has been increasing nearly twice as fast as summer temperature, but studies of warming effects on plants have focused on species that are photosynthetically active in summer. The terrestrial orchid Tipularia discolor is leafless in summer and acquires C primarily in winter. The optimum temperature for photosynthesis in T. discolor is higher than the maximum temperature throughout most of its growing season, and therefore growth can be expected to increase with warming. Contrary to this hypothesis, experimental warming negatively affected reproductive fitness (number of flowering stalks, flowers, fruits) and growth (change in leaf area from 2010 to 2012) in T. discolor. Temperature in June-July was critical for flowering, and mean July temperature greater than 29 °C (i.e., 2.5 °C above ambient) eliminated reproduction. Warming of 1.2 °C delayed flowering by an average of 10 days and fruiting by an average of 5 days. Warming of 4.4 °C reduced relative growth rates by about 60%, which may have been partially caused by the direct effects of temperature on photosynthesis and respiration. Warming indirectly increased vapor pressure deficit (VPD) by 0.2-0.5 kPa, and leaf-to-air VPD over 1.3 kPa restricted stomatal conductance of T. discolor to 10-40% of maximum conductance. These results highlight the need to account for changes in VPD when estimating temperature responses of plant species under future warming scenarios. Increasing temperature in the future will likely be an important limiting factor to the distribution of T. discolor, especially along the southern edge of its range.

  11. Effect of soya lecithin on the enzymatic system of the white-rot fungi Anthracophyllum discolor.

    Science.gov (United States)

    Bustamante, M; González, M E; Cartes, A; Diez, M C

    2011-01-01

    The present work optimized the initial pH of the medium and the incubation temperature for ligninolytic enzymes produced by the white-rot fungus Anthracophyllum discolor. Additionally, the effect of soya lecithin on mycelial growth and the production of ligninolytic enzymes in static batch cultures were evaluated. The critical micelle concentration of soya lecithin was also studied by conductivity. The effects of the initial pH (3, 4, and 5) and incubation temperature (20, 25, and 30°C) on different enzymatic activities revealed that the optimum conditions to maximize ligninolytic activity were 26°C and pH 5.5 for laccase and manganese peroxidase (MnP) and 30°C and pH 5.5 for manganese-independent peroxidase (MiP). Under these culture conditions, the maximum enzyme production was 10.16, 484.46, and 112.50 U L(-1) for laccase, MnP, and manganese-independent peroxidase MiP, respectively. During the study of the effect of soya lecithin on A. discolor, we found that the increase in soya lecithin concentration from 0 to 10 g L(-1) caused an increase in mycelial growth. On the other hand, in the presence of soya lecithin, A. discolor produced mainly MnP, which reached a maximum concentration of 30.64 ± 4.61 U L(-1) after 25 days of incubation with 1 g L(-1) of the surfactant. The other enzymes were produced but to a lesser extent. The enzymatic activity of A. discolor was decreased when Tween 80 was used as a surfactant. The critical micelle concentration of soya lecithin calculated in our study was 0.61 g L(-1).

  12. Influence of the bleaching interval on the luminosity of long-term discolored enamel-dentin discs.

    Science.gov (United States)

    Zaugg, Lucia K; Lenherr, Patrik; Zaugg, Judith B; Weiger, Roland; Krastl, Gabriel

    2016-04-01

    The aim of this study is to investigate the influence of changing the sodium perborate-tetrahydrate (PBS-4) at a 4-day interval versus no change after 16 days of internal bleaching. Two hundred and ten bovine enamel-dentin discs were discolored for 3.5 years with 14 different endodontic materials. All groups with a discoloring index of ∆E (mean) ≥ 5.5 were included in the present investigation: ApexCal (APCA), MTA white + blood (WMTA+BL), Portland cement + blood (PC+BL), blood (BL), MTA gray (GMTA), MTA gray + blood (GMTA+BL), Ledermix (LED), and triple antibiotic paste containing minocycline (3Mix). Fourteen specimens of each group were randomly assigned into two treatment groups: (1) no change of the PBS-4 (n = 7); (2) change of the PBS-4 every 4 days (n = 7). Color measurements were taken at 10 different time intervals and the L*a*b* values were recorded with a spectrophotometer (VITA Easyshade® compact). In the group 3Mix, significantly better results were achieved by changing the bleaching agent every 4 days (P = 0.0049; q = 0.04), while the group WMTA+BL indicated better results by no change of the bleaching agent (P = 0.0222, q = 0.09). All remaining groups showed no statistical difference between the two treatment procedures. Moderate discolorations can be successfully treated without changing the bleaching agent over a period of 16 days. Changing the sodium perborate-tetrahydrate every 4 days is preferred in case of severe discolored enamel-dentin discs only. This approach may offer a reduced number of clinical appointments and a secondary cost reduction to the patient.

  13. Seasonal variation in radiocesium concentrations in three tree species

    International Nuclear Information System (INIS)

    Garten, C.R. Jr.; Briese, L.A.; Sharitz, R.R.; Gentry, J.B.

    1975-01-01

    Radiocesium concentrations in leaves and stems of black willow (Salix nigra), wax myrtle (Myrica cerifera), and tag alder (Alnus serrulata) trees inhabiting a floodplain contaminated by production-reactor effluents were measured over 1 year. In willow and myrtle trees, leaf radiocesium levels were highest in the spring and declined during the growing season; stem levels remained relatively unchanged or exhibited a slight increase. Seasonal changes in alder tree parts depended on the site examined. The relationship among component parts was essentially consistent across species and collecting sites in the summer. The radiocesium concentrations in order of rank were: roots greater than or equal to leaves greater than stems. Species differences in component-part radiocesium levels were dependent on the part sampled and the collecting site examined. Mean soil to plant-part concentration factors in summer ranged from 0.9 to 7.6, and species means across leaves, stems, and roots averaged 2.1, 3.8, and 6.2 for alder, willow, and myrtle trees, respectively

  14. "The Wind in the Willows" and the Style of Romance

    Science.gov (United States)

    Gill, R. B.

    2012-01-01

    The style of Kenneth Grahame's "The Wind in the Willows" arises from an alternative vision and choice of values characteristic of romance. Romance seeks fulfillment beyond the consequences of everyday relationships and the constrictions of ordinary life. Causal relationships give way to lists of independent items, unmotivated outcomes, and…

  15. Potential benefits of commercial willow Short Rotation Coppice (SRC) for farm-scale plant and invertebrate communities in the agri-environment

    Energy Technology Data Exchange (ETDEWEB)

    Rowe, Rebecca L.; Hanley, Mick E.; Goulson, Dave; Clarke, Donna J.; Doncaster, C. Patrick; Taylor, Gail [University of Southampton, Faculty of Natural and Environmental Sciences, Life Sciences Building, Southampton, S017 1BJ (United Kingdom)

    2011-01-15

    The cultivation of bioenergy crops (BECs) represents a significant land-use change in agri-environments, but their deployment has raised important issues globally regarding possible impacts on biodiversity. Few studies however, have systematically examined the effect of commercial scale bioenergy plantations on biodiversity in agri-ecosystems. In this study we investigate how the abundance and diversity of two key components of farmland biodiversity (ground flora and winged invertebrates) varied between mature willow Short Rotation Coppice (SRC) and two alternative land-use options (arable crops and set-aside land). Although the abundance of winged invertebrates was similar across all land-uses, taxonomic composition varied markedly. Hymenoptera and large Hemiptera (>5 mm) were more abundant in willow SRC than in arable or set-aside. Similarly although plant species richness was greater in set-aside, our data show that willow SRC supports a different plant community to the other land-uses, being dominated by competitive perennial species such as Elytrigia repens and Urtica dioica. Our results suggest that under current management practices a mixed farming system incorporating willow SRC can benefit native farm-scale biodiversity. In particular the reduced disturbance in willow SRC allows the persistence of perennial plant species, potentially providing a stable refuge and food sources for invertebrates. In addition, increased Hymenoptera abundance in willow SRC could potentially have concomitant effects on ecosystem processes, as many members of this Order are important pollinators of crop plants or otherwise fulfil an important beneficial role as predators or parasites of crop pests. (author)

  16. The Influence of Log Felling Season on the Extent of Discoloration in Rubberwood Sawn Timber during the Kiln Drying Process

    Directory of Open Access Journals (Sweden)

    Jegatheswaran Ratnasingam

    2016-04-01

    Full Text Available This study investigated the effects of felling season on the discoloration of rubberwood sawn timber during conventional kiln drying. The samples were collected throughout the year 2015 to monitor the variation of free sugars and starch content in the rubberwood logs. Two batches of logs, one from the rainy season and the other from the dry season, were felled and sawn for experimentation. The findings showed that discoloration was more prominent in sawn timber obtained from logs felled during the dry season. The amount of free sugars and starch in the logs had a strong influence on the extent of discoloration in the rubberwood sawn timber during the kiln drying process. A higher amount of free sugars and starch in the logs felled during the dry season increased the incidence of blue stain on these logs. The results of this study conclusively showed that discoloration in rubberwood can be minimized by the choice of log felling season and the use of an appropriate drying technique, which will inevitably improve the aesthetic appeal of the wood.

  17. Genetic variation in the endangered Southwestern Willow Flycatcher

    Science.gov (United States)

    Busch, Joseph; Miller, Mark P.; Paxton, E.H.; Sogge, M.K.; Keim, Paul

    2000-01-01

    The Southwestern Willow Flycatcher (Empidonax traillii extimus) is an endangered Neotropical migrant that breeds in isolated remnants of dense riparian habitat in the southwestern United States. We estimated genetic variation at 20 breeding sites of the Southwestern Willow Flycatcher (290 individuals) using 38 amplified fragment length polymorphisms (AFLPs). Our results suggest that considerable genetic diversity exists within the subspecies and within local breeding sites. Statistical analyses of genetic variation revealed only slight, although significant, differentiation among breeding sites (Mantel's r = 0.0705, P UPGMA cluster analysis of the AFLP markers indicates that extensive gene flow has occurred among breeding sites. No one site stood out as being genetically unique or isolated. Therefore, the small level of genetic structure that we detected may not be biologically significant. Ongoing field studies are consistent with this conclusion. Of the banded birds that were resighted or recaptured in Arizona during the 1996 to 1998 breeding seasons, one-third moved between breeding sites and two-thirds were philopatric. Low differentiation may be the result of historically high rangewide diversity followed by recent geographic isolation of breeding sites, although observational data indicate that gene flow is a current phenomenon. Our data suggest that breeding groups of E. t. extimus act as a metapopulation.

  18. A Natural History Summary and Survey Protocol for the Southwestern Willow Flycatcher

    Science.gov (United States)

    Sogge, Mark K.; ,; Ahlers, Darrell; ,; Sferra, Susan J.; ,

    2010-01-01

    The Southwestern Willow Flycatcher (Empidonax traillii extimus) has been the subject of substantial research, monitoring, and management activity since it was listed as an endangered species in 1995. When proposed for listing in 1993, relatively little was known about the flycatcher's natural history, and there were only 30 known breeding sites supporting an estimated 111 territories rangewide (Sogge and others, 2003a). Since that time, thousands of presence/absences surveys have been conducted throughout the historical range of the flycatcher, and many studies of its natural history and ecology have been completed. As a result, the ecology of the flycatcher is much better understood than it was just over a decade ago. In addition, we have learned that the current status of the flycatcher is better than originally thought: as of 2007, the population was estimated at approximately 1,300 territories distributed among approximately 280 breeding sites (Durst and others, 2008a). Concern about the Southwestern Willow Flycatcher on a rangewide scale was brought to focus by Unitt (1987), who described declines in flycatcher abundance and distribution throughout the Southwest. E. t. extimus populations declined during the 20th century, primarily because of habitat loss and modification from activities, such as dam construction and operation, groundwater pumping, water diversions, and flood control. In 1991, the U.S. Fish and Wildlife Service (USFWS) designated the Southwestern Willow Flycatcher as a candidate category 1 species (U.S. Fish and Wildlife Service, 1991). In July 1993, the USFWS proposed to list E. t. extimus as an endangered species and to designate critical habitat under the Act (U.S. Fish and Wildlife Service, 1993). A final rule listing E. t. extimus as endangered was published in February 1995 (U.S. Fish and Wildlife Service, 1995); critical habitat was designated in 1997 (U.S. Fish and Wildlife Service, 1997). The USFWS Service released a Recovery Plan for

  19. Whitening dentifrice and tooth surface discoloration : a systematic review and meta-analysis

    NARCIS (Netherlands)

    Soeteman, G.D.; Valkenburg, C.; van der Weijden, G.A.; van Loveren, C.; Bakker, E.W.P.; Slot, D.E.

    2018-01-01

    Objective: The aim of this systematic review was to assess the effect of a whitening dentifrice (WDF) relative to a regular dentifrice (RDF) on the reduction of natural extrinsic tooth surface discoloration (ETD). Materials and metods: The MEDLINE-PubMed, Cochrane-CENTRAL and EBSCO-Dentistry and

  20. Evaluating the impact of three incentive programs on the economics of cofiring willow biomass with coal in New York State

    International Nuclear Information System (INIS)

    Tharakan, P.J.; Volk, T.A.; Lindsey, C.A.; Abrahamson, L.P.; White, E.H.

    2005-01-01

    Plantations of fast-growing willow shrubs are being promoted as a source quality biomass feedstock for bioenergy and bioproducts in New York State (NY). In the near-term, cofiring of the feedstock--in combination with other woody biomass--with coal in existing utility power boilers is considered to be the most promising conversion method for energy generation. Despite the clear technological viability and associated environmental benefits, cofiring of willow has not been widely adopted. The relatively high production cost of the willow feedstock, which is over twice that of coal, is the primary reason for this lack of interest. Taxes that account for some of the social costs of using coal and/or incentives that appropriate value for some of the social benefits of using willow are essential for eliminating most or the entire current price differential. This paper presents an integrated analysis of the economics of power generation from cofiring willow biomass feedstock with coal, from the perspective of the grower, aggregator and the power plant. Emphasis is placed on analyzing the relative impact of a green premium price, a closed-loop biomass tax credit, and payments to growers under the proposed Conservation Reserve Program (CRP) harvesting exemption policy. The CRP payments reduced the delivered cost of willow by 36-35%, to $1.90 GJ -1 and $1.70 GJ -1 , under current and increased yield conditions, respectively. These prices are still high, relative to coal. Other incentives are required to ensure commercial viability. The required levels of green premium price (0.4-1.0 cents kWh -1 ) and biomass tax credit (0.75-2.4 cents kWh -1 ) vary depending on whether the incentives were being applied by themselves or in combination, and whether current yield or potential increased yields were being considered. In the near term, cofiring willow biomass and coal can be an economically viable option for power generation in NY if the expected overall beneficial effects

  1. Predictive models of biomass for poplar and willow. Short rotation coppice in the United Kingdom

    Energy Technology Data Exchange (ETDEWEB)

    Brewer, A.C.; Morgan, G.W.; Poole, E.J.; Baldwin, M.E.; Tubby, I. (Biometrics, Surveys and Statistics Division, Forest Research, Farnham (United Kingdom))

    2007-07-01

    A series of forty-nine experimental trials on short rotation coppice (SRC) were conducted throughout the United Kingdom using a selection of varieties of poplar and willow with the aim of evaluating their performance for wood fuel production under a representative range of UK conditions. Observations on the crops and on a range of site and climatic conditions during the growth of the crops were taken over two three-year cutting cycles. These observations were used to develop a suite of empirical models for poplar and willow SRC growth and yield from which systems were constructed to provide a- priori predictions of biomass yield for any site in the UK with known characteristics (predictive yield models), and estimates of biomass yield from a standing crop (standing biomass models). The structure of the series of field trials and the consequent approach and methodology used in the construction of the suite of empirical models are described, and their use in predicting biomass yields of poplar and willow SRC is discussed. (orig.)

  2. Discoloration and tyrosinase activity in Agaricus bisporus fruit bodies infected with various pathogens

    NARCIS (Netherlands)

    Soler-Rivas, C.; Arpin, N.; Olivier, J.M.; Wichers, H.J.

    2000-01-01

    The discoloration of A. bisporus after infection by Pseudomonas tolaasii, P. gingeri, P. agarici, P. reactans, Verticillium fungicola and Trichoderma harzianum can be distinguished by chromametric measurements. Infection with P. tolaasii caused a specific colour change, independent of the bacterial

  3. An optical investigation of dentinal discoloration due to commonly endodontic sealers, using the transmitted light polarizing microscopy and spectrophotometry.

    Science.gov (United States)

    Suciu, Ioana; Ionescu, Ecaterina; Dimitriu, Bogdan Alexandru; Bartok, Ruxandra Ioana; Moldoveanu, Georgiana Florentina; Gheorghiu, Irina Maria; Suciu, Ileana; Ciocîrdel, Mihai

    2016-01-01

    The aim of this study was to establish the degree of tooth crown staining by commonly used endodontic sealers. Crown discolorations by tooth canal sealers [AH Plus (Dentsply DeTrey Gmbh, Konstanz, Germany); Endofill (Produits Dentaires SA, Vevey, Switzerland); Apexit (Dentsply DeTrey Gmbh, Konstanz, Germany); and MTA Fillapex (Angelus, Londrina, Brazil)] were tested on extracted human premolars. The samples were divided into five groups of five samples each, after root canal sealing. Five teeth were used as control groups. The spectrophotometric method was performed in order to quantify in terms of color change of the coronal part (it was also recorded a track on how the color changes over time). For the microscopic study of the extracted dental specimens subjected to this study, polarized transmitted light microscopy was used. This method involves the development of special microscopic preparations, called "thin sections". In our case, the thin section was performed on 20 prepared and obturated recently extracted teeth. The degree of discoloration was determined after one week and three months using spectrophotometry and polarized light microscopy. All sealers usually cause some degree of discoloration on the cervical aspect of the crowns that increases in time. AH Plus and Endofill caused the greatest discoloration, followed by Apexit and MTA Fillapex.

  4. EFFECT OF TEMPERATURE ON THE BIOLOGY OF TUBEROLACHNUS SALIGNUS (GMELIN (STERNORRHYNCHA: APHIDIDAE ON (SALIX ALBA

    Directory of Open Access Journals (Sweden)

    Nıhal ÖZDER

    2008-07-01

    Full Text Available The development time, survivoship and reproduction of Tuberolachnus salignus (Gmelin( Lachninae: Lachnini were studied on Salix alba at fi ve constant temperatures (17.5°C, 20°C, 22.5°C, 25°C and 27.5°C . The developmental time of immature stages ranged from 17.00 days at 17.5°C to 12.21 days at 25°C on Salix alba. The total percentage of survivorship of immature stages varied from 50% and 70% 17.5°C -20°C on S. alba. The largest r m valueoccurred with 0.2540 at 20°C on S. alba. The mean generation time of the population ranged from 13.595 days at 22.5°C to 19.60 days at 17.5°C on S. alba. The optimal temperature for Tuberolachnus salignus was 20°C.

  5. Extremely shallow spawning of perch (Perca fluviatilis L.): the roles of sheltered bays, dense semi-terrestrial vegetation and low visibility in deeper water

    Czech Academy of Sciences Publication Activity Database

    Čech, Martin; Peterka, Jiří; Říha, Milan; Vejřík, Lukáš; Jůza, Tomáš; Kratochvíl, Michal; Draštík, Vladislav; Muška, Milan; Znachor, Petr; Kubečka, Jan

    2012-01-01

    Roč. 406, December (2012), 09p1-09p12 ISSN 1961-9502 R&D Projects: GA ČR(CZ) GA206/06/1371; GA ČR(CZ) GP206/09/P266; GA MZe(CZ) QH81046 Institutional support: RVO:60077344 Keywords : Římov Reservoir * Chabařovice Lake * SCUBA diving * Reed Canarygrass Phalaris arudinacea * Goat Willow Salix caprea * egg strands Subject RIV: EG - Zoology Impact factor: 0.467, year: 2012

  6. Cultural Resources Survey and Archeological Inventory of the Proposed Carrollton Revetment Project, Orleans Parish, Louisiana, Phase 1. Volume 1 of 2

    Science.gov (United States)

    2004-11-01

    The proposed project area has a humid sub- kin ash (Fraxinus profunda ), green ash (Frax- tropical climate with prevailing southerly winds. inus...leans on June 27, 1967, is 39’ (1020 F) (Magill Salix nigra Black willow 1990:6; Trahan 1989; Peters and Beall 2002). Fraxinus profunda Pumpkin ash...In addition, the Internet radio show "New bait blue glass jar embossed "BROM... /EM...," Orleans Nostalgia" references radio advertise- refers to a

  7. The root anchorage ability of Salix alba var. tristis using a pull-out test

    African Journals Online (AJOL)

    user

    2011-11-21

    Nov 21, 2011 ... In the last few years, Beijing Forestry University (China) and the University of Natural Resources and applied Life. Sciences (Austria) have examined the suitabilities and capabilities of soil bioengineering techniques under the local condition of Beijing. One of the plant species used was Salix alba var. tristis.

  8. The effect of time between curing and tea immersion on composite resin discoloration.

    Science.gov (United States)

    Esmaeili, Behnaz; Afkhami, Solaleh; Abolghasemzadeh, Faezeh

    2018-01-01

    One common cause for the replacement of a composite restoration is discoloration. This in vitro study evaluated the effect of tea solution on the discoloration of 3 types of composites at different timepoints after curing. For this study, 150 disc-shaped specimens of 3 types of composite resin-a nanohybrid (Filtek Z350), a microhybrid (Filtek Z250), and a microfilled material (Heliomolar)-were prepared. Specimens were randomly divided into 5 subgroups (n = 10) according to the type of composite and the time from curing to immersion in a tea solution (none [immersed immediately], 1 hour, 6 hours, 12 hours, or 24 hours postcuring). The color for all specimens was measured before and after immersion in tea. Color change (ΔE*) for all specimens was measured, and a ΔE* value of less than 3.3 was considered clinically acceptable. Analysis of variance and a post hoc Tukey test were used to analyze the data (α = 0.05). Immediately after curing, the levels of composite discoloration were deemed clinically acceptable (ΔE* composites, the greatest color change was found immediately after curing (P composite resin specimens was significantly greater than that of Heliomolar specimens (P composite group than in the Heliomolar group (P composites were not significantly different from each other (P > 0.05), except with 12-hour postcure immersion. The results suggest that patients should avoid the intake of staining foods or beverages for at least 12 hours after placement of a composite resin restoration, although this restriction may be reduced to 1 hour for microfilled composite resins.

  9. Production of Probiotic Drink Using Pussy willow and Echium amoenum Extracts

    Directory of Open Access Journals (Sweden)

    Mahbobeh Eksiri

    2017-06-01

    Full Text Available Background and Objective: Nowadays, due to the lack of lactose and cholesterol, demand for consumption of non-dairy probiotic products is increasing. Probiotic drinks mixed with medicinal plant have great beneficial effect on human health. The main problems of non-dairy probiotic drinks are lack of nutrients for the growth of probiotics and bad taste of the product. The aim of this study was to produce a probiotic medicinal plant drink with favorable physicochemical, viability and sensory properties.Material and Methods: Probiotic drink prepared by Pussy willow and Echium amoenum extract (0.5 % w v-1, for each extract or together, Lactobacillus casei and Lactobacillus rhamnosus (108 CFU ml-1, individually and their combination. Glucose and whey powder (0.2% were used as a source of nutrition for the probiotics, and apple juice (20 and 30% was added to improve the taste of drink. The level of glucose was adjusted to reach the brix of 13 g100 g-1. Ascorbic acid (0.05% was used to improve micro-aerophilic conditions. The pH, acidity, glucose and viability of probiotic bacteria as well as the sensory properties of the prepared drink were investigated during 28 days at 4°C.Results and Conclusion: Based on the results, the treatment containing L. casei, Pussy willow, Echium amoenum and 30% apple juice due to the highest probiotic viability and the treatment containing Lactobacillus rhamnosus, Pussy willow, Echium amoenum and 30% apple juice because of higher total acceptance score, proper pH and acidity values were selected as the best treatments.Conflict of interest: The authors declare no conflict of interest.

  10. Uptake of cadmium from hydroponic solutions by willows (Salix spp ...

    African Journals Online (AJOL)

    DR. NJ TONUKARI

    2011-11-16

    Nov 16, 2011 ... which indicated that cadmium uptake across the plasma membrane was ... to cadmium pollution in water-soil-plant systems because .... plants were separated into roots and shoots, blotted dry with paper tissue .... Analysis of the kinetic constants for cadmium uptake ..... proteins (Welch and Norvell, 1999).

  11. The laser-induced discoloration of stonework; a comparative study on its origins and remedies.

    Science.gov (United States)

    Pouli, P; Fotakis, C; Hermosin, B; Saiz-Jimenez, C; Domingo, C; Oujja, M; Castillejo, M

    2008-12-01

    For understanding the phenomena associated with the discoloration observed in some cases of infrared laser cleaned stonework surfaces, a comparative study of three different types and morphologies of pollution encrustation and stone substrates was undertaken. Fragments originating from monuments with historic and/or artistic value, bearing homogeneous thin soiling on Pentelic marble (Athens, Greece), thick encrustation on Hontoria limestone (Burgos, Spain) and compact thin crust on gypsum decorations (Athens, Greece), have been studied on the basis of their composition and origin, together with the conditions that may induce yellowing effects upon their laser cleaning with IR wavelengths. While irradiation in the UV (i.e. at 355 nm) could not effectively remove the encrustations studied, irradiation at 1,064 nm was found efficient to remove all the studied pollution accumulations. Discoloration towards yellow was evident in all cases and at different levels, including the samples with intentional patination layer. To the limit of Raman detection no chemical alterations were detected on the irradiated areas while the presence of yellow polar compounds in all the pollution crusts studied supports the argument that the discoloration of the stone surfaces upon their IR irradiation may be due to the uncovering of existing yellow layers as result of the migration of these compounds inwards to the original stone surface. To correct and/or prevent such undesired coloration the use of IR and UV radiation both in sequential and synchronous mode was considered, with positive results.

  12. Corneal edema and permanent blue discoloration of a silicone intraocular lens by methylene blue.

    Science.gov (United States)

    Stevens, Scott; Werner, Liliana; Mamalis, Nick

    2007-01-01

    To report a silicone intraocular lens (IOL) stained blue by inadvertent intraoperative use of methylene blue instead of trypan blue and the results of experimental staining of various lens materials with different concentrations of the same dye. A "blue dye" was used to enhance visualization during capsulorhexis in a patient undergoing phacoemulsification with implantation of a three-piece silicone lens. Postoperatively, the patient presented with corneal edema and a discolored IOL. Various IOL materials were experimentally stained using methylene blue. Sixteen lenses (4 silicone, 4 hydrophobic acrylic, 4 hydrophilic acrylic, and 4 polymethylmethacrylate) were immersed in 0.5 mL of methylene blue at concentrations of 1%, 0.1%, 0.01%, and 0.001%. These lenses were grossly and microscopically evaluated for discoloration 6 and 24 hours after immersion. The corneal edema resolved within 1 month after the initial surgical procedure. After explantation, gross and microscopic analyses of the explanted silicone lens revealed that its surface and internal substance had been permanently stained blue. In the experimental study, all of the lenses except the polymethylmethacrylate lenses were permanently stained by methylene blue. The hydrophilic acrylic lenses showed the most intense blue staining in all dye concentrations. This is the first clinicopathological report of IOL discoloration due to intraocular use of methylene blue. This and other tissue dyes may be commonly found among surgical supplies in the operating room and due diligence is necessary to avoid mistaking these dyes for those commonly used during ocular surgery.

  13. Study on the effect of stabilizers on discoloring of PVC by irradiation

    International Nuclear Information System (INIS)

    Peng Chaorong; Wang Jingxia; Chen Zhuping; Liu Siyang

    2009-01-01

    Composites of polyvinyl chloride (PVC) filled with stabilizers was irradiated by 60 Co γ-ray. The effect of different stabilizers on the radiation-induced discoloring of PVC was investigated and the structures of irradiated PVC were characterized by ultraviolet spectrum (UV) and Fourrier transfer infrared spectrum (FT-IR). It shows that the stabilizers including calcium stearate/zinc stearate, epoxidised oil, phosphite and photostabilizer can improve the property of resistance to radiation-induced discoloring of PVC, and phosphite can improve the property of resistance to radiation-induced oxidation of PVC. Among the stabilizers, formulations with calcium stearate/zinc stearate show high transparency and excellent property of resistance to radiation at same absorbed dose. Change rate of yellow index of formulation (calcium stearate: zinc stearate=2:1) has been determined to be 8.3% at the absorbed dose of 30 kGy. It has been found that the structures of PVC with numbers of conjugated double bond or α, β-unsaturated ketone are formed after irradiation by γ-rays. (authors)

  14. Uptake, removal, accumulation, and phytotoxicity of 4-chlorophenol in willow trees

    DEFF Research Database (Denmark)

    Ucisik, Ahmed Süheyl; Trapp, Stefan

    2008-01-01

    of implementing phytoremediation as a treatment method for 4-CP contamination was investigated. Willows were exposed to 4-CP levels ≤79.9 mg/L in hydroponic solution. The transpiration of the trees was used to determine toxic effects. Almost no inhibition of transpiration was detected at concentrations ≥15 mg...

  15. Arbuscular mycorrhizal fungi associated with Populus-Salix stands in a semiarid riparian ecosystem

    Science.gov (United States)

    Beauchamp, Vanessa B.; Stromberg, J.C.; Stutz, J.C.

    2006-01-01

    ??? This study examined the activity, species richness, and species composition of the arbuscular mycorrhizal fungal (AMF) community of Populus-Salix stands on the Verde River (Arizona, USA), quantified patterns of AMF richness and colonization along complex floodplain gradients, and identified environmental variables responsible for structuring the AMF community. ??? Samples from 61 Populus-Salix stands were analyzed for AMF and herbaceous composition, AMF colonization, gravimetric soil moisture, soil texture, per cent organic matter, pH, and concentrations of nitrate, bicarbonate phosphorus and exchangeable potassium. ??? AMF species richness declined with stand age and distance from and elevation above the channel and was positively related to perennial species cover and richness and gravimetric soil moisture. Distance from and elevation above the active channel, forest age, annual species cover, perennial species richness, and exchangeable potassium concentration all played a role in structuring the AMF community in this riparian area. ??? Most AMF species were found across a wide range of soil conditions, but a subset of species tended to occur more often in hydric areas. This group of riparian affiliate AMF species includes several not previously encountered in the surrounding Sonoran desert. ?? New Phytologist (2006).

  16. Conversion from cropland to short rotation coppice willow and poplar: Accumulation of soil organic carbon

    Science.gov (United States)

    Georgiadis, Petros; Stupak, Inge; Vesterdal, Lars; Raulund-Rasmussen, Karsten

    2015-04-01

    Increased demand for bioenergy has intensified the production of Short Rotation Coppice (SRC) willow and poplar in temperate zones. We used a combined chronosequence and paired plot approach to study the potential of SRC willow and poplar stands to increase the soil carbon stock compared to stocks of the previous arable land-use. The study focused on well-drained soils. We sampled soil from 30 SRC stands in Denmark and southern Sweden including soils from their adjacent arable fields. The 18 willow and 12 poplar stands formed a chronosequence ranging between 4 and 29 years after conversion. The soil was sampled both with soil cores taken by fixed depths of 0-5, 5-10, 10-15, 15-25, and 25-40 cm and by genetic horizons from soil pits to 1m depth. The aim of the study was to estimate the difference and the ratio between soil carbon contents of the SRC and annual crop land and analyze the results as a chronosequence to examine the effect of age after conversion on the difference. Covariates such as soil type, fertilization type and harvest frequency were also taken into account. Preliminary results suggest an overall increase in carbon stocks over time with average accumulation rates ranging from 0.25 to 0.4 Mg ha-1 yr-1 in willow and poplar stands. Poplar stands had higher rates of C gain, probably due to less frequent harvesting. The differences in carbon between the SRC and the paired cropland were initially negative but changed to positive over time, implying loss of carbon after conversion and a later gain in soil carbon with stand age. Pairwise differences ranged from -25 Mg C ha-1 to 37 Mg C ha-1 for the top 40 cm. The carbon stock ratio of the SRC stand to the arable land was estimated to minimize the effect of site-related factors. The results of this analysis suggested that the ratio increased significantly with age after conversion for the top 10 cm of the soil, both for poplar and willow. A slight increase with age was also noticed at the deeper depths, but

  17. Sorption of lipophilic organic compunds to wood and implications for their environmental fate

    DEFF Research Database (Denmark)

    Trapp, Stefan; Miglioranza, S.B.; Mosbæk, Hans

    2001-01-01

    The sorption from water to wood (KWood) of 10 organic chemicals (logKOW, 1.48-6.20) was experimentally determined for oak (Quercus robur) and basket willow (Salix viminalis). Linear regression yielded log KWood ) -0.27 (( 0.25) + 0.632 (( 0.063)log KOW for oak (r ) 0.90, n ) 27) and log KWood ) -...... time. If metabolism inside the stem occurs, wood can serve as a “safe sink” for environmental chemicals. This might be of use in phytoremediation....

  18. Application of electrochemically generated ozone to the discoloration and degradation of solutions containing the dye Reactive Orange 122

    International Nuclear Information System (INIS)

    Santana, Mario H.P.; Da Silva, Leonardo M.; Freitas, Admildo C.; Boodts, Julien F.C.; Fernandes, Karla C.; De Faria, Luiz A.

    2009-01-01

    Aqueous solutions containing the commercial azo dye Reactive Orange 122 (RO122) were ozonated in acid and alkaline conditions. Ozone was electrochemically generated using a laboratory-made electrochemical reactor and applied using semi-batch conditions and a column bubble reactor. A constant ozone application rate of 0.25 g h -1 was used throughout. Color removal and degradation efficiency were evaluated as function of ozonation time, pH and initial dye concentration by means of discoloration kinetics and COD-TOC removal. Experimental findings revealed that pH affects both discoloration kinetics and COD-TOC removal. A single pseudo-first-order kinetic rate constant, k obs , for discoloration was found for ozonation carried out in alkaline solutions, contrary to acidic solutions where k obs depends on ozonation time. COD-TOC removal supports degradation of RO122 is more pronounced for alkaline conditions. Evaluation of the oxidation feasibility by means of the COD/TOC ratio indicates that the ozonation process in both acid and alkaline conditions leads to a reduction in recalcitrance of the soluble organic matter

  19. Indução de calos friáveis em explantes foliares de Salix (Salyx humboldtiana Willd Induction of friable callus in leaf explants of Salix (Salyx humboldtiana Willd

    Directory of Open Access Journals (Sweden)

    Breno Régis Santos

    2005-06-01

    Full Text Available O salix é uma árvore ornamental adaptada a terrenos úmidos, sendo considerada uma espécie importante para a recomposição de áreas ciliares degradadas. A madeira pode ser empregada na indústria em geral. Apesar de produzir grande quantidade de sementes, estas não possuem alta percentagem de germinação. O presente trabalho teve como objetivo realizar o estabelecimento in vitro de salix através da indução e formação de calos friáveis em explantes foliares, visando a uma posterior regeneração de plantas para a propagação massal desta espécie. Explantes foliares foram inoculados em meio de cultura MS (MURASHIGE & SKOOG, 1962, acrescido de diferentes concentrações de ácido 2,4-diclorofenoxiacético (0; 1,0; 2,0; 4,0; 6,0; 8,0; 10,0 e 12,0mg L-1 e combinações entre ácido naftalenoacético e benzilaminopurina, ambos em concentrações de 0,0; 1,0; 2,0; 4,0; 6,0 e 8,0mg L-1. Os resultados demonstram que explantes inoculados na ausência de reguladores de crescimento não apresentam a formação de calos friáveis. Significativa produção de calos friáveis (90% é obtida utilizando-se 6,0mg L-1 de ácido 2,4-diclorofenoxiacético. A utilização de concentração individual de ácido naftalenoacético ou benzilaminopurina, além de induzir a calogênese também foi capaz de promover rizogênese.Salix is an ornamental tree adapted to humid soils being considered an important species used in depleted areas. Its wood may be used in all kinds of industries. Although the production of seeds is large, the germination is reduced. The objective of this work was to establish salix in vitro through the induction and formation of friable callus of leaf explants to provide future regeneration of plants for mass propagation of the species. Leaf explants were inoculated in MS medium (MURASHIGE & SKOOG, 1962 supplemented with different concentrations of 2-4-diclorofenoxiacetic acid (0; 1.0; 2.0; 4.0; 6.0; 8.0 and 12.0mg L-1 and combinations

  20. Effects of summer and winter harvesting on element phytoextraction efficiency of Salix and Populus clones planted on contaminated soil.

    Science.gov (United States)

    Kubátová, Pavla; Száková, Jiřina; Břendová, Kateřina; Kroulíková-Vondráčková, Stanislava; Mercl, Filip; Tlustoš, Pavel

    2018-04-16

    The clones of fast-growing trees (FGTs) were investigated for phytoextraction of soil contaminated with risk elements (REs), especially Cd, Pb, and Zn. As a main experimental factor, the potential effect of biomass harvesting time was assessed. The field experiment with two Salix clones (S1 - (Salix schwerinii × Salix viminalis) × S. viminalis, S2 - S. × smithiana) and two Populus clones (P1 - Populus maximowiczii × Populus nigra, P2 - P. nigra) was established in April 2009. Shoots of all clones were first harvested in February 2012. After two further growing seasons, the first half of the trees was harvested in September 2013 before leaf fall (summer harvest) and the second half in February 2014 (winter harvest). Remediation factors (RFs) for all clones and all REs (except Pb for clone S1) were higher in the summer harvest. The highest annual RFs for Cd and for Zn (1.34 and 0.67%, respectively) were found for clone S2 and were significantly higher than other clones. Although no increased mortality of trees harvested in the summer was detected in the following season, the effect of summer harvesting on the phytoextraction potential of FGTs clones should be investigated in long-term studies.

  1. The level of invasion of the willow-poplar floodplain forests of Danube lowland

    International Nuclear Information System (INIS)

    Botkova, K.; Petrasova, K.

    2015-01-01

    Invasions of neophyte plant species are considered as one of the major threats to the diversity of natural ecosystems including floodplain forests. The aims of our study were to find out if there is a significant increase in the number and cover of neophyte species in the willow-poplar floodplain forests of Danube lowland over time. The level of invasion of the willow-poplar floodplain forests was evaluated from 1950 to the present time using Kruskal-Wallis non-parametric ANOVA. According to the analysis results, along the time gradient there is a significant increase in the number and cover of neophytes among analysed periods. This result is not caused by increasing biodiversity, because the number of native species significantly decreased. Therefor it is necessary to look for reasons of this situation in deteriorating condition of floodplain biotopes. (authors)

  2. Photocatalytic discoloration of reactive blue 5g dye in the presence of mixed oxides and with the addition of iron and silver

    International Nuclear Information System (INIS)

    Souza, M.C.P; Lenzi, G.G.; Jorge, L.M.M.; Santos, O.A.A.; Colpini, L.M.S.

    2011-01-01

    This work reports the use of cerium-titania-alumina-based systems modified with Ag and Fe by the wetness impregnation method for the discoloration of blue 5G dye. The techniques employed to characterize the photocatalysts were: temperature-programmed reduction (TPR), X-ray diffraction (XRD), specific surface area, average pore volume, and average pore diameter. The characterization results indicated that the photocatalysts had different crystalline structures and textural properties. Discoloration with the mixed oxide photocatalyst CeO 2 -TiO 2 -Al 2 O 3 gave a result similar to that of TiO 2 . On the other hand, the addition of Ag and Fe to the mixed oxide increased the discoloration and reaction rates of reactive blue 5G dyes. (author)

  3. Photocatalytic discoloration of reactive blue 5g dye in the presence of mixed oxides and with the addition of iron and silver

    Energy Technology Data Exchange (ETDEWEB)

    Souza, M.C.P; Lenzi, G.G.; Jorge, L.M.M.; Santos, O.A.A. [Universidade Estadual de Maringa (UEM), PR (Brazil). Dept. de Engenharia Quimica; Colpini, L.M.S. [Universidade Federal do Parana (UFPR), Palotina, PR (Brazil). Curso Superior de Tecnologia em Biocombustiveis

    2011-07-15

    This work reports the use of cerium-titania-alumina-based systems modified with Ag and Fe by the wetness impregnation method for the discoloration of blue 5G dye. The techniques employed to characterize the photocatalysts were: temperature-programmed reduction (TPR), X-ray diffraction (XRD), specific surface area, average pore volume, and average pore diameter. The characterization results indicated that the photocatalysts had different crystalline structures and textural properties. Discoloration with the mixed oxide photocatalyst CeO{sub 2}-TiO{sub 2}-Al{sub 2}O{sub 3} gave a result similar to that of TiO{sub 2}. On the other hand, the addition of Ag and Fe to the mixed oxide increased the discoloration and reaction rates of reactive blue 5G dyes. (author)

  4. The effect of limited availability of N or water on C allocation to fine roots and annual fine root turnover in Alnus incana and Salix viminalis.

    Science.gov (United States)

    Rytter, Rose-Marie

    2013-09-01

    The effect of limited nitrogen (N) or water availability on fine root growth and turnover was examined in two deciduous species, Alnus incana L. and Salix viminalis L., grown under three different regimes: (i) supply of N and water in amounts which would not hamper growth, (ii) limited N supply and (iii) limited water supply. Plants were grown outdoors during three seasons in covered and buried lysimeters placed in a stand structure and filled with quartz sand. Computer-controlled irrigation and fertilization were supplied through drip tubes. Production and turnover of fine roots were estimated by combining minirhizotron observations and core sampling, or by sequential core sampling. Annual turnover rates of fine roots water availability. Fine root production (treatments in Salix; i.e., absolute length and biomass production increased in the order: water limited treatment effects were detected for fine roots 1-2 mm. Proportionally more C was allocated to fine roots (≤2 mm) in N or water-limited Salix; 2.7 and 2.3 times the allocation to fine roots in the unlimited regime, respectively. Estimated input to soil organic carbon increased by ca. 20% at N limitation in Salix. However, future studies on fine root decomposition under various environmental conditions are required. Fine root growth responses to N or water limitation were less pronounced in Alnus, thus indicating species differences caused by N-fixing capacity and slower initial growth in Alnus, or higher fine root plasticity in Salix. A similar seasonal growth pattern across species and treatments suggested the influence of outer stimuli, such as temperature and light.

  5. Tratamiento de un efluente textil mediante electrooxidación-Salix babylonica

    OpenAIRE

    Sánchez Sánchez, Hilda Alejandra

    2016-01-01

    A nivel mundial, la industria textil es considerada una de las principales fuentes de descarga que afectan la calidad del agua debido al gran volumen que emplea en sus procesos y al uso de una amplia gama de colorantes sintéticos. En esta investigación se evaluó el tratamiento de un agua residual textil mediante un sistema acoplado de electrooxidación-Salix babylonica usando electrodos DDB. En el estudio, se construyó una celda electroquímica en batch, utilizando 5 electrodos paralelos vertic...

  6. Biomass power for rural development: Phase 2. Technical progress report, April 1--June 30, 1998

    Energy Technology Data Exchange (ETDEWEB)

    Neuhauser, E.

    1998-11-01

    The project undertaken by the Salix Consortium is a multi-phased, multi-partner endeavor. Phase-1 focused on initial development and testing of the technology and agreements necessary to demonstrate commercial willow production in Phase-2. The Phase-1 objectives have been successfully completed: preparing final design plans for two utility pulverized coal boilers, developing fuel supply plans for the project, obtaining power production commitments from the power companies for Phase-2, obtaining construction and environmental permits, and developing an experimental strategy for crop production and power generation improvements needed to assure commercial success. The R and D effort also addresses environmental issues pertaining to introduction of the willow energy system. Beyond those Phase-1 requirements the Consortium has already successfully demonstrated cofiring at Greenidge Station and developed the required nursery capacity for acreage scale-up. This past summer 105 acres were prepared in advance for the spring planting in 1998. Having completed the above tasks, the Consortium is well positioned to begin Phase-2. In phase-2 every aspect of willow production and power generation from willow will be demonstrated. The ultimate objective of Phase-2 is to transition the work performed under the Rural Energy for the Future project into a thriving, self-supported energy crop enterprise.

  7. Water Quality Changes in a Short-Rotation Woody Crop Riparian Buffer

    Science.gov (United States)

    Rosa, D.; Clausen, J.; Kuzovkina, J.

    2016-12-01

    Converting riparian buffers in agricultural areas from annual row crops to short rotation woody crops (SRWCs) grown for biofuel can provide both water quality benefits and a financial incentive for buffer adoption among agricultural producers. A randomized complete block design was used to determine water quality changes resulting from converting plots previously cultivated in corn to SRWC willow (Salix. spp) adjacent to a stream in Storrs, CT. Both overland flow and ground water samples were analyzed for total nitrogen (TN), nitrate + nitrite (NO2+NO3-N), and total phosphorus (TP). Overland flow was also analyzed for suspended solids concentration (SSC). Lower (p = 0.05) concentrations of TN (56%) and TP (61%) were observed in post-coppice surface runoff from willow plots than from corn plots. Shallow ground water concentrations at the edge of willow plots were lower in TN (56%) and NO3+NO2-N (64%), but 35% higher in TP, than at the edge of corn plots. SSC was also lower (72%) in overland flow associated with willow compared to corn. The treatment had no effect on discharge or mass export. These results suggest conversion from corn to a SRWC in a riparian area can provide water quality benefits similar to those observed in restored and established buffers.

  8. Importance of the 2014 Colorado River Delta pulse flow for migratory songbirds: Insights from foraging behavior

    Science.gov (United States)

    Darrah, Abigail J.; Greeney, Harold F.; van Riper, Charles

    2017-01-01

    The Lower Colorado River provides critical riparian areas in an otherwise arid region and is an important stopover site for migrating landbirds. In order to reverse ongoing habitat degradation due to drought and human-altered hydrology, a pulse flow was released from Morelos Dam in spring of 2014, which brought surface flow to dry stretches of the Colorado River in Mexico. To assess the potential effects of habitat modification resulting from the pulse flow, we used foraging behavior of spring migrants from past and current studies to assess the relative importance of different riparian habitats. We observed foraging birds in 2000 and 2014 at five riparian sites along the Lower Colorado River in Mexico to quantify prey attack rates, prey attack maneuvers, vegetation use patterns, and degree of preference for fully leafed-out or flowering plants. Prey attack rate was highest in mesquite (Prosopis spp.) in 2000 and in willow (Salix gooddingii) in 2014; correspondingly, migrants predominantly used mesquite in 2000 and willow in 2014 and showed a preference for willows in flower or fruit in 2014. Wilson’s warbler (Cardellina pusilla) used relatively more low-energy foraging maneuvers in willow than in tamarisk (Tamarix spp.) or mesquite. Those patterns in foraging behavior suggest native riparian vegetation, and especially willow, are important resources for spring migrants along the lower Colorado River. Willow is a relatively short-lived tree dependent on spring floods for dispersal and establishment and thus spring migrants are likely to benefit from controlled pulse flows.

  9. Climate impact and energy efficiency from electricity generation through anaerobic digestion or direct combustion of short rotation coppice willow

    International Nuclear Information System (INIS)

    Ericsson, Niclas; Nordberg, Åke; Sundberg, Cecilia; Ahlgren, Serina; Hansson, Per-Anders

    2014-01-01

    Highlights: • Using LCA, CHP from willow use in biogas was compared with direct combustion. • Direct combustion was ninefold more energy-efficient. • Biogas had a much greater cooling effect on global mean surface temperature. • The effects of soil carbon changes on temperature over time differed. • Biogas had long-term temperature effects, direct combustion short-term effects. - Abstract: Short rotation coppice willow is an energy crop used in Sweden to produce electricity and heat in combined heat and power plants. Recent laboratory-scale experiments have shown that SRC willow can also be used for biogas production in anaerobic digestion processes. Here, life cycle assessment is used to compare the climate impact and energy efficiency of electricity and heat generated by these measures. All energy inputs and greenhouse gas emissions, including soil organic carbon fluxes were included in the life cycle assessment. The climate impact was determined using time-dependent life cycle assessment methodology. Both systems showed a positive net energy balance, but the direct combustion system delivered ninefold more energy than the biogas system. Both systems had a cooling effect on the global mean surface temperature change. The cooling impact per hectare from the biogas system was ninefold higher due to the carbon returned to soil with the digestate. Compensating the lower energy production of the biogas system with external energy sources had a large impact on the result, effectively determining whether the biogas scenario had a net warming or cooling contribution to the global mean temperature change per kWh of electricity. In all cases, the contribution to global warming was lowered by the inclusion of willow in the energy system. The use of time-dependent climate impact methodology shows that extended use of short rotation coppice willow can contribute to counteract global warming

  10. Extracellular enzyme activity in a willow sewage treatment system.

    Science.gov (United States)

    Brzezinska, Maria Swiontek; Lalke-Porczyk, Elżbieta; Kalwasińska, Agnieszka

    2012-12-01

    This paper presents the results of studies on the activity of extra-cellular enzymes in soil-willow vegetation filter soil which is used in the post-treatment of household sewage in an onsite wastewater treatment system located in central Poland. Wastewater is discharged from the detached house by gravity into the onsite wastewater treatment system. It flows through a connecting pipe into a single-chamber septic tank and is directed by the connecting pipe to a control well to be further channelled in the soil-willow filter by means of a subsurface leaching system. Soil samples for the studies were collected from two depths of 5 cm and 1 m from three plots: close to the wastewater inflow, at mid-length of the plot and close to its terminal part. Soil samples were collected from May to October 2009. The activity of the extra-cellular enzymes was assayed by the fluorometric method using 4-methylumbelliferyl and 7-amido-4-methylcoumarin substrate. The ranking of potential activity of the assayed enzymes was the same at 5 cm and 1 m soil depths, i.e. esterase > phosphmomoesterase > leucine-aminopeptidase > β-glucosidase > α-glucosidase. The highest values of enzymatic activity were recorded in the surface layer of the soil at the wastewater inflow and decreased with increasing distance from that point.

  11. Construction of Spectral Discoloration Model for Red Lead Pigment by Aging Test and Simulating Degradation Experiment

    Directory of Open Access Journals (Sweden)

    Jinxing Liang

    2016-01-01

    Full Text Available The construction of spectral discoloration model, based on aging test and simulating degradation experiment, was proposed to detect the aging degree of red lead pigment in ancient murals and to reproduce the spectral data supporting digital restoration of the ancient murals. The degradation process of red lead pigment under the aging test conditions was revealed by X-ray diffraction, scanning electron microscopy, and spectrophotometer. The simulating degradation experiment was carried out by proportionally mixing red lead and lead dioxide with referring to the results of aging test. The experimental result indicated that the pure red lead was gradually turned into black lead dioxide, and the amount of tiny particles of the aging sample increased faced with aging process. Both the chroma and lightness of red lead pigment decreased with discoloration, and its hue essentially remains unchanged. In addition, the spectral reflectance curves of the aging samples almost started rising at about 550 nm with the inflection moving slightly from about 570 nm to 550 nm. The spectral reflectance of samples in long- and in short-wavelength regions was fitted well with the logarithmic and linear function. The spectral discoloration model was established, and the real aging red lead pigment in Dunhuang murals was measured and verified the effectiveness of the model.

  12. DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY

    Directory of Open Access Journals (Sweden)

    E. G. Sannikova

    2016-01-01

    Full Text Available The aim of the study is to develop new methods of identification and quantitative determination of rutin in the leaves of Salix triandra L. with thin-layer chromatography. The digital processing of the chromatograms was performed using «Sorbfil Videodensitometer» computer program (Krasnodar. For the chromatography analysis we used plates «Sorbfil» PTSH-P-A-UV, mobile phase: n-butanol-glacial acetic acid-water (4:1:1, detecting reagent – ammonia vapors. The rutin is detected as yellow spots with Rf 0.64±0.02. The detection limit is 0.5 μg/μl. The regression equation has the form: S=4.75×103 m. With the use of this method we found that the composition of rutin in leaves of Salix triandra was in the range 1.81±0.06% on the air-dry raw material (relative standard deviation RSD%=2.5%; the measurement error E=3.12%. The method is sensitive, linear, has a small error, and does not require expensive equipment.

  13. Vegetationsfilter för rening av lakvatten - kväveaspekter

    OpenAIRE

    Wennman, Pär

    1999-01-01

    Short rotation energy forests with willows (Salix spp) have come into increased use in Sweden for treatment of different types of waste water, sludge and ash. The vegetation does not only have a cleaning effect on the water, it is also appaerent that the water, sludge or ash often function as fertiliser for the fast growing crop. Leachate water from municipal landfills in the methanogenic phase can be cleaned with short rotation forests through a so called vegetation filter. In a small scale ...

  14. Host phylogeny and nutrient content drive galler diversity and abundance on willows

    Czech Academy of Sciences Publication Activity Database

    Volf, Martin; Kadlec, J.; Butterill, Philip T.; Novotný, Vojtěch

    2017-01-01

    Roč. 42, č. 5 (2017), s. 685-688 ISSN 0307-6946 R&D Projects: GA ČR GB14-36098G Grant - others:GA JU(CZ) 04-152/2016/P Institutional support: RVO:60077344 Keywords : defensive traits * herbivores * Salix Subject RIV: EH - Ecology, Behaviour OBOR OECD: Ecology Impact factor: 1.771, year: 2016 http://onlinelibrary.wiley.com/doi/10.1111/een.12420/abstract

  15. Frost-related dieback of Swedish and Estonian Salix plantations due to pathogenic and ice nucleation-active bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Cambours, M.A.

    2004-07-01

    During the past decade, important dieback has been observed in short-rotation forestry plantations of Salix viminalis and S. dasyclados in Sweden and Estonia, plantations from which the isolation of ice nucleation-active (INA) and pathogenic bacteria has also been reported. This thesis investigates the connection between bacterial infection and frost as a possible cause for such damage, and the role played by internal and external factors (e.g. plant frost sensitivity, fertilisation) in the dieback observed. Bacterial floras isolated from ten Salix clones growing on fertilised/unfertilised mineral soil or nitrogen-rich organic soil, were studied. Culturable bacterial communities present both in internal necrotic tissues and on the plant surface (i.e. epiphytes) were isolated on two occasions (spring and autumn). The strains were biochemically characterised (with gram, oxidase and fluorescence tests), and tested for ice nucleation-activity. Their pathogenic properties were studied with and without association to a freezing stress. Certain strains were eventually identified with BIOLOG plates and 16S rRNA analysis. A high number of culturable bacterial strains was found in the plant samplings, belonging mainly to Erwinia and Sphingomonas spp.; pathogenic and INA communities being mostly Erwinia-, Sphingomonas- and Xanthomonas-like. The generally higher plant dieback noted in the field on nutrient-rich soils and for frost sensitive clones was found connected to higher numbers of pathogenic and INA bacteria in the plants. We thus confirm Salix dieback to be related to a synergistic effect of frost and bacterial infection, possibly aggravated by fertilisation.

  16. Cryopreservation of Populus trichocarpa and Salix using dormant buds with recovery by grafting or direct rooting

    Science.gov (United States)

    Populus trichocarpa and Salix can be successfully cryopreserved by using dormant scions as the source explants. These scions (either at their original moisture content of 48 to 60% or dried to 30%) were slowly cooled to –35 degree Celsius, transferred to the vapor phase of liquid nitrogen (LNV,-160...

  17. Designing metallic iron based water filters: Light from methylene blue discoloration.

    Science.gov (United States)

    Btatkeu-K, B D; Tchatchueng, J B; Noubactep, C; Caré, S

    2016-01-15

    Available water filtration systems containing metallic iron (Fe(0) filters) are pragmatically designed. There is a lack of sound design criteria to exploit the full potential of Fe(0) filters. A science-based design relies on valuable information on processes within a Fe(0) filter, including chemical reactions, hydrodynamics and their relation to the performance of the filter. The aim of this study was to establish a simple method to evaluate the initial performance of Fe(0) filters. The differential adsorptive affinity of methylene blue (MB) onto sand and iron oxide is exploited to characterize the evolution of a Fe(0)/sand system using the pure sand system as operational reference. Five systems were investigated for more than 70 days: pure sand, pure Fe(0), Fe(0)/sand, Fe(0)/pumice and Fe(0)/sand/pumice. Individual systems were characterized by the extent of changes in pH value, iron breakthrough, MB breakthrough and hydraulic conductivity. Results showed that for MB discoloration (i) pure sand was the most efficient system, (ii) hybrid systems were more sustainable than the pure Fe(0) system, and (iii) the pores of used pumice are poorly interconnected. Characterizing the initial reactivity of Fe(0) filters using MB discoloration has introduced a powerful tool for the exploration of various aspects of filter design. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Development of short-rotation willow coppice systems for environmental purposes in Sweden

    International Nuclear Information System (INIS)

    Mirck, Jaconette; Verwijst, Theo; Isebrands, J.G.; Ledin, Stig

    2005-01-01

    During the last three decades, driving forces behind the development of short-rotation willow coppice (SRWC) in Sweden have been changing from a primary focus on biomass production towards emphasis on environmental applications. In most cases, current commercial SRWC practice is geared towards a combination of biomass production for energy purposes and environmental goals. The latter goals range from decreasing the impact of specific contaminants in the environment to organic waste handling in a recycling system in urban and/or agricultural areas. Where biomass production and pollutant management overlap, the science of phytoremediation has its practical application. Through phytoremediation, waste products that previously have been a burden for society can be used as valuable resources to increase short-rotation willow biomass production. In this paper we will present the terminology and definitions of different types of phytoremediation. We also give an overview of five different cases of phytoremediation activities with a potential for large-scale implementation. Some of the types of activities are already commercially used in Sweden; others seem promising but still need further development. (Author)

  19. High doses of ethylenediurea (EDU) as soil drenches did not increase leaf N content or cause phytotoxicity in willow grown in fertile soil.

    Science.gov (United States)

    Agathokleous, Evgenios; Paoletti, Elena; Manning, William J; Kitao, Mitsutoshi; Saitanis, Costas J; Koike, Takayoshi

    2018-01-01

    Ground-level ozone (O 3 ) levels are nowadays elevated in wide regions of the Earth, causing significant effects on plants that finally lead to suppressed productivity and yield losses. Ethylenediurea (EDU) is a chemical compound which is widely used in research projects as phytoprotectant against O 3 injury. The EDU mode of action remains still unclear, while there are indications that EDU may contribute to plants with nitrogen (N) when the soil is poor in N and the plants have relatively small leaf area. To reveal whether the N content of EDU acts as a fertilizer to plants when the soil is not poor in N and the plants have relatively large total plant leaf area, willow plants (Salix sachalinensis Fr. Schm) were exposed to low ambient O 3 levels and treated ten times (9-day interval) with 200mL soil drench containing 0, 800 or 1600mg EDU L -1 . Fertilizer was added to a nutrient-poor soil, and the plants had an average plant leaf area of 9.1m 2 at the beginning of EDU treatments. Indications for EDU-induced hormesis in maximum electron transport rate (J max ) and ratio of intercellular to ambient CO 2 concentration (C i :C a ) were observed at the end of the experiment. No other EDU-induced effects on leaf greenness and N content, maximum quantum yield of photosystem II (F v /F m ), gas exchange, growth and matter production suggest that EDU did not act as N fertilizer and did not cause toxicity under these experimental conditions. Copyright © 2017 Elsevier Inc. All rights reserved.

  20. Southwestern willow flycatchers (Empidonax traillii extimus) in a grazed landscape: factors influencing brood parasitism

    Science.gov (United States)

    Katherine M. Brodhead; Scott H. Stoleson; Deborah M. Finch

    2007-01-01

    Brood parasitism by Brown-headed Cowbirds (Molothrus ater; hereafter "cowbirds") is an important factor contributing to the endangered status of the Southwestern Willow Flycatcher (Empidonax traillii extimus, hereafter "flycatcher"). We report on factors that influence brood parasitism on the flycatcher using...

  1. Heavy metal accumulation in trees growing on contaminated sites in Central Europe

    International Nuclear Information System (INIS)

    Unterbrunner, R.; Puschenreiter, M.; Sommer, P.; Wieshammer, G.; Tlustos, P.; Zupan, M.; Wenzel, W.W.

    2007-01-01

    Metal-accumulating woody species have been considered for phytoextraction of metal-contaminated sites. We investigated Zn and Cd accumulation in tissues of adult trees and associated herbaceous species collected from contaminated areas in Central Europe. We found considerable Cd and Zn accumulation in various willow, poplar and birch species with up to 116 mg Cd kg -1 and 4680 mg Zn kg -1 in leaves of Salix caprea. Annual variation of Cd and Zn concentrations in leaves of Salix caprea were small, indicating that data obtained in different years can be compared. Metal concentrations in leaves were not related to total (aqua regia) or labile (1 M NH 4 NO 3 extract) concentrations in soil but the accumulation factors (leaf concentration: soil concentration) for Cd and Zn followed an inverse log type function. Metal partitioning between tissues showed a minimum in the wood, with increasing concentrations of Cd and Zn towards the leaves and fine roots. - Adult field-grown Salix caprea, Populus tremula and other tree species accumulate up to 4680 mg Zn kg -1 and 116 mg Cd kg -1 in their leaves

  2. Heavy metal accumulation in trees growing on contaminated sites in Central Europe

    Energy Technology Data Exchange (ETDEWEB)

    Unterbrunner, R. [University of Natural Resources and Applied Life Sciences, Vienna - BOKU, Department of Forest and Soil Sciences, Peter Jordan Strasse 82, A-1190 Vienna (Austria); Puschenreiter, M. [University of Natural Resources and Applied Life Sciences, Vienna - BOKU, Department of Forest and Soil Sciences, Peter Jordan Strasse 82, A-1190 Vienna (Austria)]. E-mail: markus.puschenreiter@boku.ac.at; Sommer, P. [University of Natural Resources and Applied Life Sciences, Vienna - BOKU, Department of Forest and Soil Sciences, Peter Jordan Strasse 82, A-1190 Vienna (Austria); Wieshammer, G. [University of Natural Resources and Applied Life Sciences, Vienna - BOKU, Department of Forest and Soil Sciences, Peter Jordan Strasse 82, A-1190 Vienna (Austria); Tlustos, P. [Czech University of Agriculture Prague, 165 21 Praha 6-Suchdol (Czech Republic); Zupan, M. [University of Ljubljana, Biotechnical Faculty, Agronomy department, Jamnikarjeva 101, 1000 Ljubljana (Slovenia); Wenzel, W.W. [University of Natural Resources and Applied Life Sciences, Vienna - BOKU, Department of Forest and Soil Sciences, Peter Jordan Strasse 82, A-1190 Vienna (Austria)

    2007-07-15

    Metal-accumulating woody species have been considered for phytoextraction of metal-contaminated sites. We investigated Zn and Cd accumulation in tissues of adult trees and associated herbaceous species collected from contaminated areas in Central Europe. We found considerable Cd and Zn accumulation in various willow, poplar and birch species with up to 116 mg Cd kg{sup -1} and 4680 mg Zn kg{sup -1} in leaves of Salix caprea. Annual variation of Cd and Zn concentrations in leaves of Salix caprea were small, indicating that data obtained in different years can be compared. Metal concentrations in leaves were not related to total (aqua regia) or labile (1 M NH{sub 4}NO{sub 3} extract) concentrations in soil but the accumulation factors (leaf concentration: soil concentration) for Cd and Zn followed an inverse log type function. Metal partitioning between tissues showed a minimum in the wood, with increasing concentrations of Cd and Zn towards the leaves and fine roots. - Adult field-grown Salix caprea, Populus tremula and other tree species accumulate up to 4680 mg Zn kg{sup -1} and 116 mg Cd kg{sup -1} in their leaves.

  3. Distribution and trends of mercury in deciduous tree cores

    International Nuclear Information System (INIS)

    Siwik, Eden I.H.; Campbell, Linda M.; Mierle, Gregory

    2010-01-01

    The distribution of total mercury (THg) within common deciduous trees and the applicability of tree cores as biomonitors of historical environmental THg trends were assessed for both contaminated and reference sites around Kingston, Ontario. Samples were collected from Acer spp., Quercus spp. Populus spp. and Salix spp. Bark and wood THg concentrations were found to be highly correlated whereas soil and wood THg concentrations were not. There were no temporal relationships for THg in dated tree rings corresponding with any other known environmental Hg trends. The shoreline speciess, Populus and Salix spp., had the greatest bark and wood Hg concentrations reaching 18 ng/g, significantly higher than for inland trees Quercus and Acer spp. with maximum values of 7 and 1.2 ng/g for bark and wood respectively. While tree cores cannot be reliably used as temporal THg biomonitors, there is promise for tree species such as Populus spp and Salix spp as spatial indicators of local long-term Hg contamination. - Total mercury trends in several deciduous trees did not follow expected environmental trends. Shoreline species (willow and popular) had higher wood THg than inland species (oak and maple).

  4. Immobilization of the white-rot fungus Anthracophyllum discolor to degrade the herbicide atrazine.

    Science.gov (United States)

    Elgueta, S; Santos, C; Lima, N; Diez, M C

    2016-12-01

    Herbicides cause environmental concerns because they are toxic and accumulate in the environment, food products and water supplies. There is a need to develop safe, efficient and economical methods to remove them from the environment, often by biodegradation. Atrazine is such herbicide. White-rot fungi have the ability to degrade herbicides of potential utility. This study formulated a novel pelletized support to immobilize the white-rot fungus Anthracophyllum discolor to improve its capability to degrade the atrazine using a biopurification system (BS). Different proportions of sawdust, starch, corn meal and flaxseed were used to generate three pelletized supports (F1, F2 and F3). In addition, immobilization with coated and uncoated pelletized supports (CPS and UPS, respectively) was assessed. UPS-F1 was determined as the most effective system as it provided high level of manganese peroxidase activity and fungal viability. The half-life (t 1/2 ) of atrazine decreased from 14 to 6 days for the control and inoculated samples respectively. Inoculation with immobilized A. discolor produced an increase in the fungal taxa assessed by DGGE and on phenoloxidase activity determined. The treatment improves atrazine degradation and reduces migration to surface and groundwater.

  5. Superação da dormência em sementes de visgueiro-do-igapó (Parkia discolor Overcoming seed dormancy in visgueiro-of-the-igapó (Parkia discolor

    Directory of Open Access Journals (Sweden)

    Sammy Aquino Pereira

    2010-03-01

    Full Text Available O presente trabalho teve como objetivo avaliar os efeitos da aplicação de tratamentos pré-germinativos de escarificação mecânica, bem como pré-tratamentos com calor, sobre a germinação de sementes de visgueiro-do-igapó (Parkia discolor Spruce ex Benth.. No primeiro experimento, sob delineamento inteiramente ao acaso, com quatro repetições, foram avaliados os pré-tratamentos: testemunha (sem pré-tratamento; desponte no lado distal; desponte no lado proximal; desponte nos lados distal e proximal; lixamento no lado distal; lixamento no lado proximal; lixamento nos lados distal e proximal; perfuração do tegumento com ferro-de-solda; e perfuração com pirógrafo. No segundo experimento, foi adotado o delineamento inteiramente casualizado, em esquema fatorial 2 (calor: seco e úmido x 4 (temperatura: 40ºC, 50ºC, 60ºC e 70ºC x 5 (período de condicionamento: 6, 16, 24, 30 e 48 horas, com três repetições. O desponte ou lixamento das sementes na porção proximal e porções proximal e distal possibilitaram uma embebição mais rápida (143-163%, aos três dias e meio e, conseqüentemente, melhores resultados de germinação (98-100%, aos quatro dias, do índice de velocidade de germinação (1,351-1,460 e do tempo médio de germinação (3 dias. Os pré-tratamentos com calor (úmido e seco, sob diferentes temperaturas, e por vários períodos de condicionamento não superaram a dormência de sementes de P. discolor.This study aimed to evaluate the effects of pregerminative mechanical scarification treatments, as well as heat pre-treatments on the germination of visgueiro-of-the-igapó (Parkia discolor Spruce ex Benth. seeds. The first experiment, with a completely randomized design and four repetitions, evaluated these pre-treatments: control (without pre-treatment; clipping at the distal portion; clipping at the proximal portion (hilum; clipping at the proximal and distal portions; scraping at the distal portion; scraping at the

  6. Use of ultrasound Doppler to determine tooth vitality in a discolored tooth after traumatic injury: its prospects and limitations

    Directory of Open Access Journals (Sweden)

    Yong-Wook Cho

    2014-02-01

    Full Text Available When a tooth shows discoloration and does not respond to the cold test or electric pulp test (EPT after a traumatic injury, its diagnosis can be even more difficult due to the lack of proper diagnostic methods to evaluate its vitality. In these case reports, we hope to demonstrate that ultrasound Doppler might be successfully used to evaluate the vitality of the tooth after trauma, and help reduce unnecessary endodontic treatments. In all three of the present cases, the teeth were discolored after traumatic injuries and showed negative responses to the cold test and EPT. However, they showed distinctive vital reactions in the ultrasound Doppler test during the whole observation period. In the first case, the tooth color returned to normal, and the tooth showed a positive response to the cold test and EPT at 10 wk after the injury. In the second case, the tooth color had returned to its normal shade at 10 wk after the traumatic injury but remained insensitive to the cold test and EPT. In the third case, the discoloration was successfully treated with vital tooth bleaching.

  7. Detection of wood discoloration in a canker fungus-inoculated Japanese cedar by neutron radiography

    International Nuclear Information System (INIS)

    Yamada, T.; Aoki, Y.; Yamato, M.; Komatsu, M.; Kusumoto, D.; Suzuki, K.; Nakanishi, T.M.

    2005-01-01

    Neutron radiography (NRG) was applied to trace the development of discolored tissue in the wood of Japanese cedar (Cryptomeria japonica) after being infected with a canker fungus. Japanese cedar seedlings were wound inoculated with a virulent and avirulent isolate of a canker fungus, Guignardia cryptomeriae. Three, 7, 13 and 22 days after the inoculation, the seedlings were irradiated with thermal neutrons. The image on the X-ray film showed that the whiteness in the image corresponded to the water content in the sample. Discolored tissue and surrounding dry zones induced by the fungal inoculation were detected as dark areas, indicating water deficiency with a high resolution. Through image analysis, the dry zones were detected as early as 3 days after inoculation. Neutron images also showed the difference in the size of water deficient parts due to the tissue damage among the treatments. The neutron beam dose used in this experiment had no effect on the growth rate of the fungus on a medium, showing that NRG is an effective method for pathological research of trees. (author)

  8. Comparative of Quercus spp. and Salix spp. for phytoremediation of Pb/Zn mine tailings.

    Science.gov (United States)

    Shi, Xiang; Wang, Shufeng; Sun, Haijing; Chen, Yitai; Wang, Dongxue; Pan, Hongwei; Zou, Yazhu; Liu, Jianfeng; Zheng, Linyu; Zhao, Xiulian; Jiang, Zeping

    2017-02-01

    A pot experiment was conducted to evaluate the feasibility of using tree seedlings for the phytoremediation of lead/zinc (Pb/Zn) mine tailings. Seedlings of three Quercus spp. (Q. shumardii, Q. phellos, and Q. virginiana) and rooted cuttings of two Salix spp. (S. matsudana and S. integra) were transplanted into pots containing 50 and 100 % Pb/Zn mine tailings to evaluate their tolerance of heavy metals. The five species showed different tolerance levels to the Pb/Zn tailings treatments. Q. virginiana was highly tolerant to heavy metals and grew normally in the Pb/Zn tailings. The root systems showed marked differences between the Quercus spp. and Salix spp., indicating that different mechanisms operated to confer tolerance of heavy metals. The maximum efficiency of photosystem II photochemistry value of the five species showed no differences among the treatments, except for Q. shumardii. All species showed low metal translocation factors (TFs). However, S. integra had significantly higher TF values for Zn (1.42-2.18) and cadmium (1.03-1.45) than did the other species. In this respect, Q. virginiana showed the highest tolerance and a low TF, implying that it is a candidate for phytostabilization of mine tailings in southern China. S. integra may be useful for phytoextraction of tailings in temperate regions.

  9. Phytotoxicity of fresh and weathered diesel and gasoline to willow and poplar trees

    DEFF Research Database (Denmark)

    Trapp, Stefan; Köhler, A.; Larsen, L.C.

    2001-01-01

    The toxicity of fresh and weathered gasoline and diesel fuel to willow and poplar trees was studied using a tree transpiration toxicity test. Soils were taken from an abandoned filling station. Concentrations in the samples were measured as the sum of hydrocarbons from C5 to C10 (gasoline) and C1...

  10. Seasonal variation in radiocaesium concentration in willow ptarmigan and rock ptarmigan in central Norway after the Chernobyl fallout

    International Nuclear Information System (INIS)

    Pedersen, H.C.; Nyboe, S.; Varskog, P.

    1998-01-01

    Radioactive caesium (20-60 kBq m -2 ) was deposited after the Chernobyl accident in the mountains of central Norway. Two sympatric ptarmigan species, willow ptarmigan Lagopus lagopus and rock ptarmigan L. mutus, inhabit this alpine ecosystem and are important game species. In 1987 and 1988, a study was carried out to try to identify factors affecting radioactive caesium concentration in these birds. Juvenile willow ptarmigan contained more radiocaesium than adults, but the two sexes did not differ in radiocaesium concentration. The radiocaesium concentration of food plants correlated with radiocaesium concentration of rock ptarmigan, and a seasonal variation in radiocaesium concentration of both ptarmigan species was seen. Rock ptarmigan contained more radiocaesium than willow ptarmigan during winter, but not in summer. This difference was related to differences in diet. The bioconcentration factor was 0·4-0·6. The aggregated transfer coefficient was 0·003-0·009 m 2 kg -1 for both species. In spite of the high deposition, the radiocaesium concentration in muscle rarely exceeded the limit recommended for human food consumption (600 Bq kg -1 ). (Copyright (c) 1998 Elsevier Science B.V., Amsterdam. All rights reserved.)

  11. Biomass direct-fired power generation system in China: An integrated energy, GHG emissions, and economic evaluation for Salix

    International Nuclear Information System (INIS)

    Wang, Changbo; Zhang, Lixiao; Chang, Yuan; Pang, Mingyue

    2015-01-01

    To gain a better understanding of the options of biomass power generation in China, this study presented an integrated energy, environmental, and economic evaluation for Salix in China, and a typical Salix direct-fired power generation system (SDPGS) in Inner Mongolia was selected for case study. A tiered hybrid life cycle assessment (LCA) model was developed to calculate the “planting-to-wire” (PTW) energy consumption, greenhouse gas (GHG) emissions, and economic cost and profit of the SDPGS, including feedstock cultivation, power plant construction and operation, and on-grid price with/without government subsidies. The results show that the PTW energy consumption and GHG emissions of Salix are 0.8 MJ/kWh and 114 g CO 2 -eq/kWh, respectively, indicating an energy payback time (EPBT) of 3.2 years. The SDPGS is not economically feasible without government subsidies. The PTW costs are dominated by feedstock cultivation. The energy saving and GHG mitigation benefits are still robust, even when the power plant runs at only 60% design capacity. For future development of biomass power in China, scientific planning is necessary to guarantee a sufficient feedstock supply. In addition, technology progress, mature industrial chains, and reasonable price setting policy are required to enable potential energy and environmental advantages of biomass power moving forward. -- Highlights: •A hybrid LCA model was used to evaluate overall performance of the SDPGS. •On-site processes dominate the “planting-to-wire” footprints. •The energy saving and GHG mitigation benefits of the SDPGS are robust. •The economic profit of the SDPGS is feeble without government subsidies. •Generating efficiency promotion has a comprehensive positive effect on the system

  12. Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride

    Directory of Open Access Journals (Sweden)

    RAPHAEL S S SOUZA

    2009-01-01

    Full Text Available Stannous chloride (SnC12 is used in nuclear medicine as a reducing agent to obtain technetium-99m-radiopharmaceuticals. It have been reported that natural products might reduce the genotoxic and cytotoxic effects related to SnC12. This work evaluated the biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli (E. coli AB1157 (wild type cultures submitted to the action of SnC12. E. coli AB1157 cultures (exponential growth phase were collected by centrifugation, washed and resuspended in 0.9%NaCl. Samples were incubated in water bath shaker with: (a SnC12 (25mg/ml, (bSalix alba extract(11.6mg/ml and (cSnC12(25mg/ml + Salix alba extract (11.6mg/ml. Incubation with 0.9% NaCl was also carried out (control. At 60 min intervals, aliquots were withdrawn, diluted, spread onto Petri dishes with solid LB medium and incubated overnight. The colonies formed were counted and the survival fractions calculated. The extract was not able to protect the E. coli cultures against the lesive action of SnC12. The extract also did not interfere with the survival of the cultures. It suggested that the substances present in the Salix alba aqueous extract did not interfere strongly with cellular metabolism and did not alter the survival fractions of E. coli AB 1157. It is speculated that this extract cannot interfere with the generation of free radicals, the possible main agent responsible for SnC12 lesive action.

  13. Polysaccharides on microsporocytes and tapetum in Rhoeo discolor. Cytochemical and autoradiographic study (/sup 3/H-glucose)

    Energy Technology Data Exchange (ETDEWEB)

    Albertini, L; Souvre, A [Ecole Nationale Superieure Agronomique, 31 - Toulouse (France); Toulouse-3 Univ., 31 (France))

    1978-01-01

    In Rhoeo discolor, we have been studying the evolution of cytoplasmic and wall polysaccharides on microsporocytes and tapetum by cytochemical and autoradiographic (6-/sup 3/H glucose) methods with particular attention to the meiocyte special wall, microspore intine, pollen generative cell wall, microsporocyte plastids and tapetum cytoplasm.

  14. Mapping of quantitative trait loci (QTL) for production, resistance and tolerance traits in Salix. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Roennberg-Waestljung, Ann Christin; Bertholdsson, Nils-Ove; Glynn, Carolyn; Weih, Martin; Aahman, Inger [SLU, Uppsala (Sweden). Dept. of Plant Biology and Forest Genetics

    2004-05-01

    Quantitative trait loci (QTL) for growth traits, water use efficiency and tolerance/resistance against metals and herbivores have been identified. A hybrid F2 population originating from a cross between a Salix dasyclados-clone (SW901290) and a S. viminalis-clone ('Jorunn') was used for the different studies in this project. The growth response was analyzed in a greenhouse experiment with two water treatments, normal and drought. In addition, three field experiments with contrasting soils and climates were established. QTL specific for each treatment or field environment but also QTL stable over the treatments or field environments were detected. Each QTL explained from 8 to 29 % of the phenotypic variation depending on trait, treatment or field environment. Clusters of QTL for different traits were mapped indicating a common genetic base or tightly-linked QTL. Stable QTL identified for dryweight can be useful tools for early selection in Salix. In a separate greenhouse experiment, with a subset of ten genotypes from the F2 population, we show that genotype is more important than irrigation treatment for production of phenolic substances as well as for resistance to herbivory by P vulgatissima.

  15. Effect of Diffusion on Discoloration of Congo Red by Alginate Entrapped Turnip (Brassica rapa Peroxidase

    Directory of Open Access Journals (Sweden)

    Afaf Ahmedi

    2015-01-01

    Full Text Available Enzymatic discoloration of the diazo dye, Congo red (CR, by immobilized plant peroxidase from turnip “Brassica rapa” is investigated. Partially purified turnip peroxidase (TP was immobilized by entrapment in spherical particles of calcium alginate and was assayed for the discoloration of aqueous CR solution. Experimental data revealed that pH, reaction time, temperature, colorant, and H2O2 concentration play a significant role in dye degradation. Maximum CR removal was found at pH 2.0, constant temperature of 40°C in the presence of 10 mM H2O2, and 180 mg/L of CR. More than 94% of CR was removed by alginate immobilized TP after 1 h of incubation in a batch process under optimal conditions. About 74% removal efficiency was retained after four recycles. Diffusional limitations in alginate beads such as effectiveness factor η, Thiele modulus Φ, and effective diffusion coefficients (De of Congo red were predicted assuming a first-order biodegradation kinetic. Results showed that intraparticle diffusion resistance has a significant effect on the CR biodegradation rate.

  16. Seismic modelling of coal bed methane strata, Willow Creek, Alberta

    Energy Technology Data Exchange (ETDEWEB)

    Richardson, S.E.; Mayer, R.; Lawton, D.C.; Langenberg, W. [Consortium for Research in Elastic Wave Exploration Seismology, Calgary, AB (Canada)

    2001-07-01

    The purpose is to determine the feasibility of applying high- resolution reflection seismic surveying to coalbed methane (CBM) exploration and development. Numerical reflection seismic methods are examined for measuring the mapping continuity and coherence of coal zones. Numerical modelling of a coal zone in Upper Cretaceous sediments near Willow Creek, Alberta indicates that seismic data that is predominantly of 100 Hz is required to map the coal zone and lateral facies variations within the deposit. For resolution of individual coal seams, a central frequency >150 Hz would be needed. 26 refs., 17 figs., 3 tabs.

  17. Microbial expression profiles in the rhizosphere of willows depend on soil contamination

    OpenAIRE

    Yergeau, Etienne; Sanschagrin, Sylvie; Maynard, Christine; St-Arnaud, Marc; Greer, Charles W

    2013-01-01

    The goal of phytoremediation is to use plants to immobilize, extract or degrade organic and inorganic pollutants. In the case of organic contaminants, plants essentially act indirectly through the stimulation of rhizosphere microorganisms. A detailed understanding of the effect plants have on the activities of rhizosphere microorganisms could help optimize phytoremediation systems and enhance their use. In this study, willows were planted in contaminated and non-contaminated soils in a greenh...

  18. Porous Carbon with Willow-Leaf-Shaped Pores for High-Performance Supercapacitors.

    Science.gov (United States)

    Shi, Yanhong; Zhang, Linlin; Schon, Tyler B; Li, Huanhuan; Fan, Chaoying; Li, Xiaoying; Wang, Haifeng; Wu, Xinglong; Xie, Haiming; Sun, Haizhu; Seferos, Dwight S; Zhang, Jingping

    2017-12-13

    A novel kind of biomass-derived, high-oxygen-containing carbon material doped with nitrogen that has willow-leaf-shaped pores was synthesized. The obtained carbon material has an exotic hierarchical pore structure composed of bowl-shaped macropores, willow-leaf-shaped pores, and an abundance of micropores. This unique hierarchical porous structure provides an effective combination of high current densities and high capacitance because of a pseudocapacitive component that is afforded by the introduction of nitrogen and oxygen dopants. Our synthetic optimization allows further improvements in the performance of this hierarchical porous carbon (HPC) material by providing a high degree of control over the graphitization degree, specific surface area, and pore volume. As a result, a large specific surface area (1093 m 2 g -1 ) and pore volume (0.8379 cm 3 g -1 ) are obtained for HPC-650, which affords fast ion transport because of its short ion-diffusion pathways. HPC-650 exhibits a high specific capacitance of 312 F g -1 at 1 A g -1 , retaining 76.5% of its capacitance at 20 A g -1 . Moreover, it delivers an energy density of 50.2 W h kg -1 at a power density of 1.19 kW kg -1 , which is sufficient to power a yellow-light-emitting diode and operate a commercial scientific calculator.

  19. Biofuel or excavation? - Life cycle assessment (LCA) of soil remediation options

    Energy Technology Data Exchange (ETDEWEB)

    Suer, Pascal; Andersson-Skoeld, Yvonne [Swedish Geotechnical Institute, 58193 Linkoeping (Sweden)

    2011-02-15

    The environmental consequences of soil remediation through biofuel or through dig-and-dump were compared using life cycle assessment (LCA). Willow (Salix viminalis) was actually grown in-situ on a discontinued oil depot, as a phytoremediation treatment. These data were used for the biofuel remediation, while excavation-and-refill data were estimated from experience. The biofuel remediation had great environmental advantages compared to the ex situ excavation remediation. With the ReCiPe impact assessment method, which included biodiversity, the net environmental effect was even positive, in spite of the fact that the wood harvest was not utilised for biofuel production, but left on the contaminated site. Impact from the Salix viminalis cultivation was mainly through land use for the short rotation coppice, and through journeys of control personnel. The latter may be reduced when familiarity with biofuel as a soil treatment method increases. The excavation-and-refill remediation was dominated by the landfill and the transport of contaminated soil and backfill. (author)

  20. Spectroscopic analysis of the role of extractives on heat-induced discoloration of black locust (Robinia pseudoacacia)

    Science.gov (United States)

    Yao Chen; Yongming Fan; Jianmin Gao; Mandla A. Tshabalala; Nicole M. Stark

    2012-01-01

    To investigate the role of extractives on heat-induced discoloration of wood, samples of black locust (Robinia pseudoacacia) wood flour were extracted with various solvents prior to heat-treatment. Analysis of their color parameters and chromophoric structures showed that the chroma value of the unextracted sample decreased while that of the...

  1. Discoloration of a red cationic dye by supported TiO2 photocatalysis

    International Nuclear Information System (INIS)

    Mounir, B.; Pons, M.N.; Zahraa, O.; Yaacoubi, A.; Benhammou, A.

    2007-01-01

    The degradation under UV, visible and sunlight irradiation of C.I. Basic Red 46 (BR 46) dye used for acrylic fibers dyeing has been studied in a lab-scale continuous system with two different immobilized TiO 2 systems. Catalyst I was based on TiO 2 particles deposited on cellulose fibers; Catalyst II combined TiO 2 particles deposited on a layer of cellulose fibers (as in Catalyst I) with a layer of carbon fibers and finally a layer of cellulose fibers. The treatment of aqueous dye solutions and industrial wastewater contaminated with the same dye has been evaluated in terms of color removal and chemical oxygen demand (COD) decrease. With UV light, aqueous solutions containing dye were decolorized slightly more rapidly with Catalyst II than with Catalyst I. Sunlight was also very effective and experiments involving sunlight irradiation showed Catalyst II to be the more efficient, giving more than 90% discoloration after 20 min of treatment. Comparing the discoloration yield by adsorption or under visible light for both catalysts, it was observed that the difference between them is below 5%. The adsorption kinetics was found to follow a second-order rate law for Catalyst I and a first-order rate law for Catalyst II. The kinetics of photocatalytic degradation under UV or sunlight were found to follow a first-order rate law for both catalytic systems. Under sunlight the COD removal yield for textile wastewater reaches 33% with Catalyst I against 93% with Catalyst II

  2. Overgrowth of fungi (endolithic hypermycosis) associated with multifocal to diffuse distinct amorphous dark discoloration of corals in the Indo-Pacific

    Science.gov (United States)

    Work, Thierry M.; Aeby, G.S.; Stanton, F.G.; Fenner, D.

    2008-01-01

    Coral disease surveys in American Samoa and Hawai‘i revealed colonies with a distinct dark discoloration affecting 20–60% of the colony surface (Fig. 1a). In some cases, tissue loss with algal infiltration was present within discolored areas. On microscopy, these lesions had marked overgrowth of the coral skeleton and tissues with septate branching structures that stained positive with Grocott’s Methenamine Silver (fungal hyphae) accompanied by necrosis and fragmentation of coral tissues (Fig. 1b). We have observed this condition grossly and microscopically in Pavona varians, Psammocora nierstraszi, and Montipora sp. in American Samoa and in Pavona maldivensis and P. varians in Hawai‘i. This condition resembles Dark Spots Disease from the Caribbean (Solano et al. 1993) that also shows endolithic hypermycosis (Galloway et al. 2007), suggesting that the association between dark discoloration of corals and overgrowth of endolithic fungi may be common (Western Atlantic, Indo-Pacific). Based on gross and microscopic morphology, tissue atrophy may precede overgrowth of endolithic fungi, but this awaits confirmation through systematic studies that monitor the development of lesions over time (pathogenesis). Using standardized terminology (Work and Aeby 2006) to describe lesions facilitates regional comparisons of coral disease.

  3. Paleoseismology of the Nephi Segment of the Wasatch Fault Zone, Juab County, Utah - Preliminary Results From Two Large Exploratory Trenches at Willow Creek

    Science.gov (United States)

    Machette, Michael N.; Crone, Anthony J.; Personius, Stephen F.; Mahan, Shannon; Dart, Richard L.; Lidke, David J.; Olig, Susan S.

    2007-01-01

    In 2004, we identified a small parcel of U.S. Forest Service land at the mouth of Willow Creek (about 5 km west of Mona, Utah) that was suitable for trenching. At the Willow Creek site, which is near the middle of the southern strand of the Nephi segment, the WFZ has vertically displaced alluvial-fan deposits >6-7 m, forming large, steep, multiple-event scarps. In May 2005, we dug two 4- to 5-m-deep backhoe trenches at the Willow Creek site, identified three colluvial wedges in each trench, and collected samples of charcoal and A-horizon organic material for AMS (acceleration mass spectrometry) radiocarbon dating, and sampled fine-grained eolian and colluvial sediment for luminescence dating. The trenches yielded a stratigraphic assemblage composed of moderately coarse-grained fluvial and debris-flow deposits and discrete colluvial wedges associated with three faulting events (P1, P2, and P3). About one-half of the net vertical displacement is accommodated by monoclinal tilting of fan deposits on the hanging-wall block, possibly related to massive ductile landslide deposits that are present beneath the Willow Creek fan. The timing of the three surface-faulting events is bracketed by radiocarbon dates and results in a much different fault chronology and higher slip rates than previously considered for this segment of the Wasatch fault zone.

  4. Phytoscreening and phytoextraction of heavy metals at Danish polluted sites using willow and poplar trees

    DEFF Research Database (Denmark)

    Nielsen, Mette Algreen; Trapp, Stefan; Rein, Arno

    2014-01-01

    The main purpose of this study was to determine typical concentrations of heavy metals (HM) in wood from willows and poplars, in order to test the feasibility of phytoscreening and phytoextraction of HM. Samples were taken from one strongly, one moderately, and one slightly polluted site and from...

  5. A habitat overlap analysis derived from Maxent for Tamarisk and the South-western Willow Flycatcher

    Science.gov (United States)

    Patricia York; Paul Evangelista; Sunil Kumar; James Graham; Curtis Flather; Thomas Stohlgren

    2011-01-01

    Biologic control of the introduced and invasive, woody plant tamarisk (Tamarix spp, saltcedar) in south-western states is controversial because it affects habitat of the federally endangered South-western Willow Flycatcher (Empidonax traillii extimus). These songbirds sometimes nest in tamarisk where floodplain-level invasion replaces native habitats. Biologic control...

  6. Fungal Distribution and Varieties Resistance to Kernel Discoloration in Korean Two-rowed Barley

    Directory of Open Access Journals (Sweden)

    Sang-Hyun Shin

    2013-09-01

    Full Text Available Barley kernel discoloration (KD leads to substantial loss in value through downgrading and discounting of malting barley. The objective of this research is to investigate fungal distribution and varieties resistance to KD in Korean two-rowed barley. Several fungal organisms including Alternaria spp., Fusarium spp., Aspergillus spp., Epicoccum spp. and Rhizopus spp. were isolated from Korean two-rowed barley representing KD. The symptoms of KD were brown and black discolorations of the lemma and palea. The most frequently detected fungal species was Alternaria spp. which exhibited 69.1% and 72.2% in 2011 and 2012, respectively. Epicoccum spp., Fusarium spp., and Aspergillus spp. were also detected. Fusarium spp., primary pathogen of barley head blight, were rarely occurred in the 2011 and their occurrence increased to 4.7% in 2012. Twenty cultivars of Korean two-rowed barely were evaluated to KD. The average percentage of KD was 8.0−36.0% in 2011 and 5.2−36.6% in 2012. Two cultivars (‘Sacheon 6’ and ‘Dajinbori’ showed KD of 6.2% to 8.8% and determined resistant, however ‘Samdobori’ and ‘Daeyeongbori’ demonstrating KD of 22.2−36.6% were highly susceptible. ‘Jinyangbori’, ‘Danwonbori’, ‘Sinhobori’ and ‘Kwangmaegbori’ showing KD of less than 15% were moderately resistant cultivar.

  7. Application of two electrical methods for the rapid assessment of freezing resistance in Salix epichloro

    Energy Technology Data Exchange (ETDEWEB)

    Tsarouhas, V.; Kenney, W.A.; Zsuffa, L. [University of Toronto, Ontario (Canada). Faculty of Forestry

    2000-09-01

    The importance of early selection of frost-resistant Salix clones makes it desirable to select a rapid and accurate screening method for assessing freezing resistance among several genotypes. Two electrical methods, stem electrical impedance to 1 and 10 khz alternating current, and electrolyte leakage of leaf tissue, were evaluated for detecting freezing resistance on three North America Salix epichloro Michx., clones after subjecting them to five different freezing temperatures (-1, -2, -3, -4, and -5 deg C). Differences in the electrical impedance to 1 and 10 kHz, and the ratio of the impedance at the two frequencies (low/high) before and after the freezing treatment (DZ{sub low}, DZ{sub high}, and DZ{sub ratio}, respectively) were estimated. Electrolyte leakage was expressed as relative conductivity (RC{sub t}) and index of injury (IDX{sub t}). Results from the two methods, obtained two days after the freezing stress, showed that both electrical methods were able to detect freezing injury in S. eriocephala. However, the electrolyte leakage method detected injury in more levels of freezing stress (-3, -4, and -5 deg C) than the impedance (-4, and -5 deg C), it assessed clonal differences in S. eriocephala freezing resistance, and it was best suited to correlate electrical methods with the visual assessed freezing injury. No significant impedance or leakage changes were found after the -1 and -2 deg C freezing temperatures. (author)

  8. The Effect of Trichoderma on Heavy Metal Mobility and Uptake by Miscanthus giganteus, Salix sp., Phalaris arundinacea, and Panicum virgatum

    Directory of Open Access Journals (Sweden)

    Malgorzata J. Kacprzak

    2014-01-01

    Full Text Available The effect of land application of biomaterials based on two strains of Trichoderma fungus on phytoremediation processes was studied. Six metals (Cd, Cr, Cu, Pb, Zn, and Ni were analysed in soil and soil leachate as well as in plant tissues. The translocation index (Ti and metal bioconcentration factors (BCF calculated for the inoculated plants were increased compared to the noninoculated control, except for Pb and Salix sp. Simultaneously, the mobilisation of metals in soil solution as an effect of biomaterials was noted. The highest values of Ti—339% (for Cr, 190% (for Ni, and 110% (for Cu—were achieved for the combination Miscanteus giganteus and Trichoderma MSO1. The results indicated that the application of fungus has positive effects on increasing the biomass, soil parameters (C, N, and P, and solubility of heavy metals in soil and therefore in enhancing phytoextraction for Miscanthus giganteus L., Panicum virgatum L., Phalaris arundinacea L., and Salix sp.

  9. Seasonal dynamics and effects of nitrogen supply rate on nitrogen and carbohydrate reserves in cutting-derived Salix viminalis plants

    Science.gov (United States)

    Lars Bollmark; Lisa Sennerby-Forsse; Tom Ericsson

    1999-01-01

    Nurient storage is an important aspect of resprouting potential and production of Salix viminalis L., a pioneer species used for biomas production in weden. Seasonal dynamics of nitrogen (N), protien, soluble arbohydrates, starches, and lipids were studied in roots, cutting, stems, and leaves during a full growth cycle induced by varying photoperiod...

  10. Resident rounds. Part III: case report: temporary traumatic discoloration from handling moss-covered firewood.

    Science.gov (United States)

    Zitelli, Kristine B; Adams, Brian B

    2013-07-01

    Pattern recognition remains a valuable tool in the accurate diagnosis of dermatologic disease. A comprehensive patient history and physical examination denote cornerstones of medical evaluation, and in our specialty, dermoscopy can supplement data gathering to allocate cutaneous eruptions into appropriate categories. We present a case of acute onset palmar discoloration occurring in an otherwise healthy patient. Correct diagnosis transpired in the clinical setting with tailored questioning based on careful examination and adjunct dermatoscopic evaluation.

  11. Ambient UV-B radiation reduces PSII performance and net photosynthesis in high Arctic Salix arctica

    DEFF Research Database (Denmark)

    Albert, Kristian Rost; Mikkelsen, Teis Nørgaard; Ro-Poulsen, H.

    2011-01-01

    , nitrogen and UV-B absorbing compounds. Compared to a 60% reduced UV-B irradiance, the ambient solar UV-B reduced net photosynthesis in Salix arctica leaves fixed in the 45° position which exposed leaves to maximum natural irradiance. Also a reduced Calvin Cycle capacity was found, i.e. the maximum rate...... across position in the vegetation. These findings add to the evidence that the ambient solar UV-B currently is a significant stress factor for plants in high Arctic Greenland....

  12. A phytotoxicity test using transpiration of willows

    DEFF Research Database (Denmark)

    Trapp, Stefan; Zambrano, Kim Cecilia; Kusk, Kresten Ole

    2000-01-01

    is expressed as % decrease after 48 and 72 h or longer compared to the initial transpiration, divided by the transpiration of control plants. More toxicity parameters are growth and water use efficiency of the plants. The sensitivity of the test was evaluated with 3,5-dichlorophenol. EC50 values between 5......A short-term acute toxicity assay for willow trees growing in contaminated solution or in polluted soil was developed and tested. The test apparatus consists of an Erlenmeyer flask with a prerooted tree cutting growing in it. Growth and reduction of transpiration are used to determine toxicity....... Transpiration is closely related to photosynthesis and growth, but is easier and faster to measure and can be measured without disturbance of the test system. Plants are grown for 24 h in uncontaminated nutrient solution before the toxicant is added to determine the initial transpiration. The loss of weight...

  13. Comparison of Different Wood Species as Raw Materials for Bioenergy

    Directory of Open Access Journals (Sweden)

    Bojana Klašnja

    2013-12-01

    Full Text Available Background and Purpose: Most projections of the global energy use predict that biomass will be an important component of primary energy sources in the coming decades. Short rotation plantations have the potential to become an important source of renewable energy in Europe because of the high biomass yields, a good combustion quality as solid fuel, ecological advantages and comparatively low biomass production costs. Materials and Methods: In this study, the wood of black locust Robinia pseudoacacia, white willow Salix alba L., poplars Populus deltoides and Populus x euramericana cl.I-214, aged eight years were examined. Immediately after the felling, sample discs were taken to assess moisture content, ash content, the width of growth rings, wood densities and calorific values, according to the standard methodology. Results:The mean values of willow, poplar and black locust wood density were 341 kg/m3, 336 kg/m3 and 602 kg/m3,respectively. The average heating values of willow poplar and black locust wood were 18.599 MJ/kg, 18.564 MJ/kg and 21.196 MJ/kg, respectively. The FVI index (average values was higher for black locust (17.186 than for poplar and willow clones, which were similar: 11.312 and 11.422 respectively. Conclusions: Black locust wood with a higher density, calorific value and ash content compared to poplar and willow wood proved to be a more suitable raw material as RES. However, it is very important, from the aspect of the application of wood of these tree species as RES, to also consider the influence of the biomass yield per unit area of the plantations established as “energy plantations”.

  14. The Potential Use of Agroforestry Community Gardens as a Sustainable Import-Substitution Strategy for Enhancing Food Security in Subarctic Ontario, Canada

    Directory of Open Access Journals (Sweden)

    Maren Oelbermann

    2013-09-01

    Full Text Available The high prevalence of food insecurity experienced by northern First Nations partially results from dependence on an expensive import-based food system that typically lacks nutritional quality and further displaces traditional food systems. In the present study, the feasibility of import substitution by Agroforestry Community Gardens (AFCGs as socio-ecologically and culturally sustainable means of enhancing food security was explored through a case study of Fort Albany First Nation in subarctic Ontario, Canada. Agroforestry is a diverse tree-crop agricultural system that has enhanced food security in the tropics and subtropics. Study sites were selected for long-term agroforestry research to compare Salix spp. (willow-dominated AFCG plots to a “no tree” control plot in Fort Albany. Initial soil and vegetative analysis revealed a high capacity for all sites to support mixed produce with noted modifications, as well as potential competitive and beneficial willow-crop interactions. It is anticipated that inclusion of willow trees will enhance the long-term productive capacity of the AFCG test plots. As an adaptable and dynamic system, AFCGs have potential to act as a more reliable local agrarian system and a refuge for culturally significant plants in high-latitude First Nation socio-ecological systems, which are particularly vulnerable to rapid cultural, climatic, and ecological change.

  15. Cd and Zn concentrations in small mammals and willow leaves on disposal facilities for dredged material

    NARCIS (Netherlands)

    Mertens, J.; Luyssaert, S.; Verbeeren, S.; Vervaeke, P; Lust, N

    2001-01-01

    Disposal sites for dredged material are often polluted with heavy metals. The uptake of Cd and Zn by small mammals and willow trees was assessed on three sites with a different pollution degree. Detailed soil sampling showed a huge variation in soil characteristics within the sites, typical for

  16. Annual variations in the solar energy conversion efficiency in a willow coppice stand

    International Nuclear Information System (INIS)

    Noronha-Sannervik, A.; Kowalik, P.

    2003-01-01

    Productivity of an experimental willow coppice forest located at Uppsala, Sweden, was monitored between 1985 and 1994. The 2.7 ha stand was planted in 1984 with a density of 20 000 cuttings per ha and was harvested three times. During the monitored period, the annual stem wood production and the cumulated values of total solar radiation during the growing season, were measured. The conversion of incoming solar radiation into stem biomass was evaluated and the results show that the solar energy conversion efficiency (ECE), for the first and fourth year of the cutting cycle, is, on average, 64% of the ECE for the second and third year of the cutting cycle. It is discussed that the low ECE of 1-year-old shoots is related to a delay in leaf canopy development at the beginning of the growing season and lack of weed control after harvest. For the 4-years-old shoots, the low ECE, is believed to be related to the increased shoot and stool mortality caused by the self-thinning process ongoing in the willow stand. It is recommended that the harvesting interval should be based on the specific development of the stand and more attention should be paid to weed control, especially in the first growing season after harvest

  17. Bioactivity and chemical constituents of Rhoeo spathacea and Rhoeo discolor, with emphasis on Rhoeo spathacea

    OpenAIRE

    Tan, Ban Lee Joash

    2017-01-01

    Rhoeo spathacea and Rhoeo discolor are two common ornamental plants from the Commelinaceae family, commonly grown in tropical countries including Malaysia and have been traditionally taken orally for the treatment of superficial mycoses, inflammations and gonorrhoea. Despite the ethnobotanical applications of both plants and their popularity as a beverage particularly in South America, relatively little work has been conducted on their bioactivity and chemical constituents, thus warranting fu...

  18. The use of short rotation willows and poplars for the recycling of saline waste waters

    Science.gov (United States)

    Jaconette Mirck; Ronald S. Jr. Zalesny; Ioannis Dimitriou; Jill A. Zalesny; Timothy A. Volk; Warren E. Mabee

    2009-01-01

    The production of high-salinity waste waters by landfills and other waste sites causes environmental concerns. This waste water often contains high concentrations of sodium and chloride, which may end up in local ground and surface waters. Vegetation filter systems comprised of willows and poplars can be used for the recycling of saline waste water. These vegetation...

  19. Dry matter losses and quality changes during short rotation coppice willow storage in chip or rod form.

    Science.gov (United States)

    Whittaker, Carly; Yates, Nicola E; Powers, Stephen J; Misselbrook, Tom; Shield, Ian

    2018-05-01

    This study compares dry matter losses and quality changes during the storage of SRC willow as chips and as rods. A wood chip stack consisting of approximately 74 tonnes of fresh biomass, or 31 tonnes dry matter (DM) was built after harvesting in the spring. Three weeks later, four smaller stacks of rods with an average weight of 0.8 tonnes, or 0.4 tonnes DM were built. During the course of the experiment temperature recorders placed in the stacks found that the wood chip pile reached 60 °C within 10 days of construction, but the piles of rods remained mostly at ambient temperatures. Dry matter losses were calculated by using pre-weighed independent samples within the stacks and by weighing the whole stack before and after storage. After 6 months the wood chip stack showed a DM loss of between 19.8 and 22.6%, and mean losses of 23.1% were measured from the 17 independent samples. In comparison, the rod stacks showed an average stack DM loss of between 0 and 9%, and between 1.4% and 10.6% loss from the independent samples. Analysis of the stored material suggests that storing willow in small piles of rods produces a higher quality fuel in terms of lower moisture and ash content; however, it has a higher fine content compared to storage in chip form. Therefore, according to the two storage methods tested here, there may be a compromise between maximising the net dry matter yield from SRC willow and the final fine content of the fuel.

  20. Willow and poplar for bioenergy on former cropland

    DEFF Research Database (Denmark)

    Georgiadis, Petros

    and water demands of the trees. The water requirements of SRWCs are generally high, and high evapotranspiration rates in both SRC willow and SRF poplar decreased deep percolation, which along with low N concentrations led to low N leaching. Excessive N leaching was only observed when SRC was fertilized......Climate change is one of the 21st century’s greatest challenges and calls for immediate action through the implementation of mitigation strategies. A shift from fossil fuel to renewable energy is a key factor for reducing greenhouse gas emissions to the atmosphere, with bioenergy being...... the predominant sector of renewables in the current European and global energy markets. Dedicated energy crops, such as short rotation woody crops (SRWC), are promising bioenergy feedstock in southern Scandinavia due to their high yields. Such cropping systems have high demands for land, water, and nutrients...

  1. Influence of all-ceramic and porcelain-fused-to-metal restorations on peri-implant gingival discoloration:a spectrophotometric comparison

    DEFF Research Database (Denmark)

    Peng, Min; Fei, Wei; Hosseini, Mandana

    2013-01-01

    discoloration scores were evaluated by clinician. SPSS17.0 was used to analyze the data. RESULTS: There was no significant difference between the all-ceramic group (3.4+1.8) and PFM group (4.9+3.4) spectrophotometrically. No significant difference was found between the all-ceramic restorations and PFM...

  2. Sampling procedure in a willow plantation for estimation of moisture content

    DEFF Research Database (Denmark)

    Nielsen, Henrik Kofoed; Lærke, Poul Erik; Liu, Na

    2015-01-01

    Heating value and fuel quality of wood is closely connected to moisture content. In this work the variation of moisture content (MC) of short rotation coppice (SRC) willow shoots is described for five clones during one harvesting season. Subsequently an appropriate sampling procedure minimising...... labour costs and sampling uncertainty is proposed, where the MC of a single stem section with the length of 10–50 cm corresponds to the mean shoot moisture content (MSMC) with a bias of maximum 11 g kg−1. This bias can be reduced by selecting the stem section according to the particular clone...

  3. Effect of HALS and antioxidant on the radiation-induced discoloration of polypropylene

    International Nuclear Information System (INIS)

    Wang Huiliang; Kong Xiangbo; Chen Wenxiu

    1999-01-01

    The effects of hindered amine light stabilizers (HALS), antioxidants and the combination of HALS and antioxidants on the radiation-induced discoloration of polypropylene (PP) are studied. The yellow index (YI) of PP added pentamethyl HALS is found to be lower than that of PP added corresponding tetramethyl HALS at the same radiation dose. The YI of PP added antioxidant Irganox 1076 is lower than that of PP added Irgafos 168 or Irganox PS-802. When Irganox 1076 and PDS are used together, the YI of PP is lower than that of Irganox 1076 or PDS used separately. YI of PP decreases with storage time after irradiation. The results are discussed

  4. The effects of first-year shoot cut back on willow biomass production during the first and second cutting cycle; Effekter av skottnedklippning efter etableringsaaret paa produktionen under foersta och andra omdrevet i salixodlingar

    Energy Technology Data Exchange (ETDEWEB)

    Verwijst, Theo; Nordh, Nils-Erik

    2010-05-15

    During the early phase of commercialisation of willow short rotation forestry in Sweden it was considered to be important to cut down the shoots after the establishment year, and this practice still is maintained today. Several reasons then were put forward for cutting down. The primary goal was to stimulate sprouting of more shoots, thereby obtaining a rapidly closing stand which could compete with weeds. Another reason was that it would enable weed control during the second year. In some of the older clones, which exhibited bow-shaped shoots, cutting down was supposed to lead to more straight growing shoots which rendered less biomass loss at harvest. In the meantime, the practice of cutting down has been abandoned in Denmark, and there are reasons to scrutinise the effects of cutting down on available biomass production figures from Swedish field trials. The main goal with this project was to try to quantify the effects of cutting down on the biomass productivity of willow during the first and later cutting cycles. Thus far, efforts in this research topic have not led to a scientifically documented quantification on the effects, and therefore a scientific basis for hands-on recommendations to willow growers is lacking. The purpose of our work consequently was to generate practical recommendations with regard to the management measure of cutting down, and the audience targeted consisted of willow growers (farmers, land-owners and entrepreneurs) who are deciding about and perform the actual management of willow stands to obtain higher yields. This information also is of large relevance for all extension workers in willow growing. Data have been collected from a field trial which was planted in Flosta, Enkoeping, in 2005. As this was the only experiment on cutting down which ran during the project period, we searched the archives which contained material from another experiment which ran from 1992 to 1996 in Ultuna, Uppsala, and from which some date were collected

  5. Microbial expression profiles in the rhizosphere of willows depend on soil contamination

    Science.gov (United States)

    Yergeau, Etienne; Sanschagrin, Sylvie; Maynard, Christine; St-Arnaud, Marc; Greer, Charles W

    2014-01-01

    The goal of phytoremediation is to use plants to immobilize, extract or degrade organic and inorganic pollutants. In the case of organic contaminants, plants essentially act indirectly through the stimulation of rhizosphere microorganisms. A detailed understanding of the effect plants have on the activities of rhizosphere microorganisms could help optimize phytoremediation systems and enhance their use. In this study, willows were planted in contaminated and non-contaminated soils in a greenhouse, and the active microbial communities and the expression of functional genes in the rhizosphere and bulk soil were compared. Ion Torrent sequencing of 16S rRNA and Illumina sequencing of mRNA were performed. Genes related to carbon and amino-acid uptake and utilization were upregulated in the willow rhizosphere, providing indirect evidence of the compositional content of the root exudates. Related to this increased nutrient input, several microbial taxa showed a significant increase in activity in the rhizosphere. The extent of the rhizosphere stimulation varied markedly with soil contamination levels. The combined selective pressure of contaminants and rhizosphere resulted in higher expression of genes related to competition (antibiotic resistance and biofilm formation) in the contaminated rhizosphere. Genes related to hydrocarbon degradation were generally more expressed in contaminated soils, but the exact complement of genes induced was different for bulk and rhizosphere soils. Together, these results provide an unprecedented view of microbial gene expression in the plant rhizosphere during phytoremediation. PMID:24067257

  6. The polyene antimycotics nystatin and filipin disrupt the plasma membrane, whereas natamycin inhibits endocytosis in germinating conidia of Penicillium discolor

    NARCIS (Netherlands)

    Leeuwen, van M.R.; Golovina, E.A.; Dijksterhuis, J.

    2009-01-01

    To investigate the differences in membrane permeability and the effect on endocytosis of the polyene antimycotics nystatin, filipin and natamycin on germinating fungal conidia. Methods and Results: The model system was Penicillium discolor, a food spoilage fungus. Filipin resulted in

  7. Discoloration of a red cationic dye by supported TiO{sub 2} photocatalysis

    Energy Technology Data Exchange (ETDEWEB)

    Mounir, B. [Ecole Superieure de Technologie de Safi, Universite Cadi Ayyad, BP 89, Route Dar Si Aissa, Safi (Morocco); Pons, M.N. [Laboratoire des Sciences du Genie Chimique, CNRS-ENSIC-INPL, 1, rue Grandville, BP 20451, F-54001 Nancy Cedex (France)], E-mail: Marie-Noelle.Pons@ensic.inpl-nancy.fr; Zahraa, O. [Departement de Chimie Physique des Reactions, ENSIC-INPL, 1, rue Grandville, BP 20451, F-54001 Nancy Cedex (France); Yaacoubi, A. [Departement de Chimie, Faculte des Sciences Semlalia, Universite Cadi Ayyad, Boulevard Prince Moulay Abdellah, BP 2390, 40000 Marrakech (Morocco); Benhammou, A. [Laboratoire d' Automatique et d' Etudes des Procedes, Faculte des Sciences Semlalia, Universite Cadi Ayyad, Boulevard Prince Moulay Abdellah, BP 2390, 40000 Marrakech (Morocco)

    2007-09-30

    The degradation under UV, visible and sunlight irradiation of C.I. Basic Red 46 (BR 46) dye used for acrylic fibers dyeing has been studied in a lab-scale continuous system with two different immobilized TiO{sub 2} systems. Catalyst I was based on TiO{sub 2} particles deposited on cellulose fibers; Catalyst II combined TiO{sub 2} particles deposited on a layer of cellulose fibers (as in Catalyst I) with a layer of carbon fibers and finally a layer of cellulose fibers. The treatment of aqueous dye solutions and industrial wastewater contaminated with the same dye has been evaluated in terms of color removal and chemical oxygen demand (COD) decrease. With UV light, aqueous solutions containing dye were decolorized slightly more rapidly with Catalyst II than with Catalyst I. Sunlight was also very effective and experiments involving sunlight irradiation showed Catalyst II to be the more efficient, giving more than 90% discoloration after 20 min of treatment. Comparing the discoloration yield by adsorption or under visible light for both catalysts, it was observed that the difference between them is below 5%. The adsorption kinetics was found to follow a second-order rate law for Catalyst I and a first-order rate law for Catalyst II. The kinetics of photocatalytic degradation under UV or sunlight were found to follow a first-order rate law for both catalytic systems. Under sunlight the COD removal yield for textile wastewater reaches 33% with Catalyst I against 93% with Catalyst II.

  8. Mycorrhizal associations as Salix repens L. communities in succession of dune ecosystems II Mycorrhizal dynamics and interactions of ectomycorrhizal and arbuscular mycorrhizal fungi

    NARCIS (Netherlands)

    Heijden, van der E.W.; Vosatka, M.

    2000-01-01

    Ectomycorrhizal (EcM) and arbuscular mycorrhizal (AM) associations of Salix repens were studied at 16 sites in different successional stages of dune ecosystems (calcareous-acidic, dry-wet) in the Netherlands. High EcM colonization, low AM colonization, and lack of differences between habitats

  9. Linkage between bacterial and fungal rhizosphere communities in hydrocarbon-contaminated soils is related to plant phylogeny.

    Science.gov (United States)

    Bell, Terrence H; El-Din Hassan, Saad; Lauron-Moreau, Aurélien; Al-Otaibi, Fahad; Hijri, Mohamed; Yergeau, Etienne; St-Arnaud, Marc

    2014-02-01

    Phytoremediation is an attractive alternative to excavating and chemically treating contaminated soils. Certain plants can directly bioremediate by sequestering and/or transforming pollutants, but plants may also enhance bioremediation by promoting contaminant-degrading microorganisms in soils. In this study, we used high-throughput sequencing of bacterial 16S rRNA genes and the fungal internal transcribed spacer (ITS) region to compare the community composition of 66 soil samples from the rhizosphere of planted willows (Salix spp.) and six unplanted control samples at the site of a former petrochemical plant. The Bray-Curtis distance between bacterial communities across willow cultivars was significantly correlated with the distance between fungal communities in uncontaminated and moderately contaminated soils but not in highly contaminated (HC) soils (>2000 mg kg(-1) hydrocarbons). The mean dissimilarity between fungal, but not bacterial, communities from the rhizosphere of different cultivars increased substantially in the HC blocks. This divergence was partly related to high fungal sensitivity to hydrocarbon contaminants, as demonstrated by reduced Shannon diversity, but also to a stronger influence of willows on fungal communities. Abundance of the fungal class Pezizomycetes in HC soils was directly related to willow phylogeny, with Pezizomycetes dominating the rhizosphere of a monophyletic cluster of cultivars, while remaining in low relative abundance in other soils. This has implications for plant selection in phytoremediation, as fungal associations may affect the health of introduced plants and the success of co-inoculated microbial strains. An integrated understanding of the relationships between fungi, bacteria and plants will enable the design of treatments that specifically promote effective bioremediating communities.

  10. Combustion quality of poplar and willow clones grown as SRC at four sites in Brandenburg, Germany

    DEFF Research Database (Denmark)

    Liu, Na; Ugilt Larsen, Søren; Jørgensen, Uffe

    2017-01-01

    The fuel quality was assessed for nine poplar clones (AF2, Androscoggin, Max1, Max3, Max4, Monviso, Muhle-Larsen, NE42, Weser6) and one willow clone (Inger) grown as short rotation coppice (SRC) on four sites in the Brandenburg area in Germany. Fuel quality was analysed in 3-year old shoots in te...

  11. Manipulating beaver (Castor canadensis) feeding responses to invasive tamarisk (Tamarix spp.).

    Science.gov (United States)

    Kimball, Bruce A; Perry, Kelly R

    2008-08-01

    To evaluate methods for promoting consumption of tamarisk plants by beavers (Castor canadensis), we determined the feeding responses by captive beavers to diets that contained tannins and sodium chloride (hereafter referred to as tamarisk diet). In two-choice tests, beavers consumed equivalent quantities of tamarisk diet and control diet. Treatment with polyethylene glycol and fructose did not increase beaver preferences for the tamarisk diet. When offered the choice of control diet and casein hydrolysate-treated control diet, beavers strongly avoided the latter, showing feeding deterring activity of casein hydrolysate. However, when tamarisk diet was the alternative to the deterrent treatment, beavers consumed similar quantities of the two diets. Finally, beaver foraging preferences for actual plant cuttings were assessed. Casein hydrolysate application to cuttings of black poplar (Populus nigra) and Scouler's willow (Salix scouleriana) reduced browsing of these highly preferred species and promoted a marked increase in browsing of tamarisk (Tamarix ramosissima). These results suggest that casein hydrolysate treatment of desirable riparian plant species such as Salix and Populus may promote beaver foraging of invasive tamarisk.

  12. Discoloration of Ancient Egyptian Mural Paintings by Streptomyces Strains and Methods of Its Removal

    Directory of Open Access Journals (Sweden)

    Akmal Ali SAKR

    2012-12-01

    Full Text Available Streptomyces isolated from mural paintings at Tell Basta and Tanis tombs were identified using 16S rDNA sequencing method. These Streptomyces strains caused discoloration of mural paintings with irreversible red stains of carotenoid pigment. A mixture of n-hexan and acetone (92:8 v/v was the best solvent for extracting and purification of red pigment from biomass of Streptomyces. Dimethyl sulfoxide (DMSO and N,N-dimethylformamide (DMF were the most effective in treatment of these red stains without changing the paintings or stone surfaces.

  13. Making Space for Red Tide: Discolored Water and the Early Twentieth Century Bayscape of Japanese Pearl Cultivation.

    Science.gov (United States)

    Ericson, Kjell

    2017-05-01

    "Red tide" has become a familiar shorthand for unusual changes in the color of ocean waters. It is intimately related both to blooms of creatures like dinoflagellates and to the devastating effects they pose to coastal fisheries. This essay tracks the early twentieth century emergence of discolored water as an aquacultural problem, known in Japan as akashio, and its trans-oceanic transformation into the terms and practices of "red tide" in the post-World War II United States. For Japan's "Pearl King" Mikimoto Kōkichi and his contacts in diverse marine scientific communities, the years-long cycle of guarding and cultivating a pearl oyster went together with the ascription of moral qualities to tiny creatures that posed a threat to farmed bayscapes of pearl monoculture. As akashio, discolored water went from curiosity to marine livestock pest, one that at times left dead pearl oysters in its wake. Red tide arose from the sustained study of the mechanisms by which changes in the biological and chemical composition of seawater might become deadly to exclusively-claimed shellfish along Japanese coastlines, but came to be seen as a way to understand aquatic manifestations of harm in other parts of the littoral world.

  14. Distribution and abundance of Least Bell’s Vireos (Vireo bellii pusillus) and Southwestern Willow Flycatchers (Empidonax traillii extimus) on the Middle San Luis Rey River, San Diego County, southern California—2017 data summary

    Science.gov (United States)

    Allen, Lisa D.; Howell, Scarlett L.; Kus, Barbara E.

    2018-04-20

    We surveyed for Least Bell’s Vireos (LBVI) (Vireo bellii pusillus) and Southwestern Willow Flycatchers (SWFL) (Empidonax traillii extimus) along the San Luis Rey River, between College Boulevard in Oceanside and Interstate 15 in Fallbrook, California (middle San Luis Rey River), in 2017. Surveys were conducted from April 13 to July 11 (LBVI) and from May 16 to July 28 (SWFL). We found 146 LBVI territories, at least 107 of which were occupied by pairs. Five additional transient LBVIs were detected. LBVIs used five different habitat types in the survey area: mixed willow, willow-cottonwood, willow-sycamore, riparian scrub, and upland scrub. Forty-four percent of the LBVIs occurred in habitat characterized as mixed willow and 89 percent of the LBVI territories occurred in areas with greater than 50 percent native plant cover. Of 16 banded LBVIs detected in the survey area, 8 had been given full color-band combinations prior to 2017. Four other LBVIs with single (natal) federal bands were recaptured and banded in 2017. Three LBVIs with single dark blue federal bands indicating that they were banded as nestlings on the lower San Luis Rey River and one LBVI with a single gold federal band indicating that it was banded as a nestling on Marine Corps Base Camp Pendleton (MCBCP) could not be recaptured for identification. One banded LBVI emigrated from the middle San Luis Rey River to the lower San Luis Rey River in 2017.One resident SWFL territory and one transient Willow Flycatcher of unknown subspecies (WIFL) were observed in the survey area in 2017. The resident SWFL territory, which was comprised of mixed willow habitat (5–50 percent native plant cover), was occupied by a single male from May 22 to June 21, 2017. No evidence of pairing or nesting activity was observed. The SWFL male was banded with a full color-combination indicating that he was originally banded as a nestling on the middle San Luis Rey River in 2014 and successfully bred in the survey area in 2016

  15. Reliable molecular differentiation of Trichuris ovis and Trichuris discolor from sheep (Ovis orientalis aries) and roe deer (Capreolus capreolus) and morphological characterisation of their females: morphology does not work sufficiently.

    Science.gov (United States)

    Vejl, Pavel; Nechybová, Stanislava; Peřinková, Pavla; Melounová, Martina; Sedláková, Vladimíra; Vašek, Jakub; Čílová, Daniela; Rylková, Kateřina; Jankovská, Ivana; Vadlejch, Jaroslav; Langrová, Iva

    2017-08-01

    The main aim of the study was to evaluate associations between morphological variability of Trichuris females from sheep and roe deer and their rDNA polymorphism in whipworm populations from the Czech Republic. The results introduced the use of new molecular markers based on the internal transcribed spacer (ITS)1-5.8S RNA-ITS2 region polymorphisms, as useful tools for the unambiguous differentiation of congeners Trichuris ovis and Trichuris discolor. These markers revealed both parasites in roe deer and in sheep; however, T. ovis females predominated in sheep while T. discolor females occurred mostly in roe deer. Additional analysis of ITS1-5.8 rRNA-ITS2 discovered the genetic uniformity of the analysed T. discolor but high haplotype variation of T. ovis. Simultaneously, molecularly designated female individuals of both species were categorised into four morphotypes (MT) on the basis of morphology of genital pore area. MT1 and MT4 (vulvar opening on everted vaginal appendage/on visible cuticular bulge) occurred only in T. ovis, MT2 (uneverted vagina-vulvar opening without any elevation) was identified only in T. discolor and MT3 (transient type of vulvar opening on a small swelling) was observed in both species. Statistical analysis of biometric data confirmed that morphology of vulva is not a reliable marker for the species determination. On the basis of the ITS1-5.8S RNA-ITS2 region variability, we carried out a phylogenetic analysis (maximum likelihood method, Hasegawa-Kishino-Yano model) which showed that T. ovis haplotypes from the Czech Republic and Ireland and T. discolor haplotypes from the Czech Republic, Spain, Iran and Japan are sister OTUs.

  16. Synthesis of carbon nanospheres using fallen willow leaves and adsorption of Rhodamine B and heavy metals by them.

    Science.gov (United States)

    Qu, Jiao; Zhang, Qian; Xia, Yunsheng; Cong, Qiao; Luo, Chunqiu

    2015-01-01

    This paper focuses on the synthesis of carbon nanospheres (CNSs) using fallen willow leaves as a low-cost precursor. The scanning electron microscopy (SEM) image and transmission electron microscopy (TEM) image demonstrated that the structure of synthesized CNSs was spherical, with a diameter of 100 nm. The crystal structure and chemical information were characterized by Raman spectrum and energy-dispersive spectrum (EDS), respectively. BET results showed that the CNSs had a larger specific surface area of 294.32 m(2) g(-1), which makes it a potentially superior adsorbent. Rh-B and heavy metal ions such as Cu(2+), Zn(2+), and Cr(6+) were used as targets to investigate the adsorption capacity of the CNSs. The effects of adsorption parameters such as adsorption equilibrium time, dose of CNSs, adsorption kinetics, and effect factors were also studied. These findings not only established a cost-effective method of synthesizing CNSs using fallen willow leaves but also broadened the potential application range of these CNSs.

  17. Are cicadas (Diceroprocta apache) both a "keystone" and a "critical-link" species in lower Colorado River riparian communities?

    Science.gov (United States)

    Andersen, Douglas C.

    1994-01-01

    Apache cicada (Homoptera: Cicadidae: Diceroprocta apache Davis) densities were estimated to be 10 individuals/m2 within a closed-canopy stand of Fremont cottonwood (Populus fremontii) and Goodding willow (Salix gooddingii) in a revegetated site adjacent to the Colorado River near Parker, Arizona. Coupled with data drawn from the literature, I estimate that up to 1.3 cm (13 1/m2) of water may be added to the upper soil layers annually through the feeding activities of cicada nymphs. This is equivalent to 12% of the annual precipitation received in the study area. Apache cicadas may have significant effects on ecosystem functioning via effects on water transport and thus act as a critical-link species in this southwest desert riverine ecosystem. Cicadas emerged later within the cottonwood-willow stand than in relatively open saltcedar-mesquite stands; this difference in temporal dynamics would affect their availability to several insectivorous bird species and may help explain the birds' recent declines. Resource managers in this region should be sensitive to the multiple and strong effects that Apache cicadas may have on ecosystem structure and functioning.

  18. Diet preference of Eurasian Beaver (Castor Fiber L., 1758 in the environment of Oderské vrchy and its influence on the tree species composition of river bank stands

    Directory of Open Access Journals (Sweden)

    Jan Dvořák

    2013-01-01

    Full Text Available This paper deals with the dietary behaviour and the tree species preference in the river bank stands in the diet of established population Eurasian Beaver (Castor fiber L., 1758 in the environment of Oderské vrchy; the objective is to assess the suitability of this environment for future development of the Eurasian Beaver and to asses the influence of the beaver’s dietary behaviour on the river bank stands. In the monitored area, the total of 5 tree species with the following preference were recorded: willow (Salix 42.2%, aspen (Populus 28%, dogwood (Comus 15.5%, birch (Betula 7.4% and alder (Alnus 6.9%. The most damaged diameter interval recorded within the all damaged tree species ranges from 2.6 to 6 cm, followed by the interval 6.1–12 cm. Over 61% of the trees felled by the beaver had a bigger diameter. The most sensitive reaction to beaver’s dietary behaviour was shown by aspen (reduction of numbers by 27.6% and by willow (reduction of numbers by 16.6% on the monitored area.

  19. A short history of anti-rheumatic therapy. II. Aspirin

    Directory of Open Access Journals (Sweden)

    P. Marson

    2011-06-01

    Full Text Available The discovery of aspirin, an antipyretic, anti-inflammatory and analgesic drug, undoubtedly represents a milestone in the history of medical therapy. Since ancient times the derivatives of willow (Salix alba were used to treat a variety of fevers and pain syndromes, although the first report dates back to 1763 when the English Reverend Edward Stone described the effect of an extract of the bark willow in treating malaria. In the XIX century many apothecaries and chemists, including the Italian Raffaele Piria and Cesare Bertagnini, developed the biological processes of extraction and chemical synthesis of salicylates, and then analyzed their therapeutic properties and pharmacokinetic and pharmacodynamic characteristics. In 1899 the Bayer Company, where Felix Hoffmann, Heinrich Dreser and Arthur Eichengrün worked, recorded acetyl-salicylic acid under the name “Aspirin”. In the XX century, besides the definition of the correct applications of aspirin in the anti-rheumatic therapy being defined, Lawrence L. Crawen identified the property of this drug as an anti-platelet agent, thus opening the way for more widespread uses in cardiovascular diseases.

  20. El Alzado de la Vivienda en Willow Road Ernö Goldfinger

    Directory of Open Access Journals (Sweden)

    Ignacio Román Santiago

    2015-05-01

    Full Text Available ResumenEl artículo propone aproximarse a la vivienda que el arquitecto húngaro Ernö Goldfinger construyó para sí mismo en 1939 en el barrio de Hampstead (Londres. El complejo y singular ejercicio en el que Goldfinger apoya gran parte de sus teorías se manifiesta en esta propuesta para Willow Road y se desvela especialmente en su fachada. Para el arquitecto es fundamental estudiar la experiencia humana del espacio arquitectónico relacionada con la envolvente del edificio y, en este sentido, sulegado teórico se expresa en los textos que escribió para la revista Architectural Review entre los años 1941 y 1942: The Sensation of Space, Urbanism and Spatial Order y Elements of Enclosed Space.Analizar este alzado enigmático provoca algunos interrogantes que son, en sí mismos, un auténtico manifi esto a favor de una arquitectura libre de toda adscripción estilística. Goldfinger actúa aquí en una mesa de laboratorio donde pone a prueba sus reflexiones sobre el orden espacial, la composición, la escala, el carácter y la construcción.De esta forma la propuesta para Willow Road no puede explicarse exclusivamente en términos funcionales ni mecanicistas, sino que precisa una visión fenomenológica y al mismo tiempo mirarlo con amplitud para empezar a descubrir sus claves. Este hogar, que puede entenderse como vivienda burguesa y también representa un ejercicio moderno, ofrece la oportunidad de desvelar un modo de hacer, muy particular, en el que algunos arquitectos desarraigados y liberados del imperante tradicionalismo inglés y de cualquier dogmatismo, dejan a un lado el itinerario que cabría esperar en aquel momento y 'contaminan' la arquitectura moderna inglesa, para producir un panorama de influencias difícil de reconocer en cualquier otra localización. La huella de esta modernidad revisada, de espíritu inequívocamente moderno pero filtrado desde una actitud clásica, influye en la siguiente generación de arquitectos ingleses

  1. MRI and unilateral NMR study of reindeer skin tanning processes.

    Science.gov (United States)

    Zhu, Lizheng; Del Federico, Eleonora; Ilott, Andrew J; Klokkernes, Torunn; Kehlet, Cindie; Jerschow, Alexej

    2015-04-07

    The study of arctic or subarctic indigenous skin clothing material, known for its design and ability to keep the body warm, provides information about the tanning materials and techniques. The study also provides clues about the culture that created it, since tanning processes are often specific to certain indigenous groups. Untreated skin samples and samples treated with willow (Salix sp) bark extract and cod liver oil are compared in this study using both MRI and unilateral NMR techniques. The two types of samples show different proton spatial distributions and different relaxation times, which may also provide information about the tanning technique and aging behavior.

  2. Evaluation of spectral light management on growth of container-grown willow oak, nuttall oak and summer red maple

    Science.gov (United States)

    Plant response to blue, red, gray or black shade cloth was evaluated with willow oak (Quercus phellos L.), Nuttall oak (Quercus nuttallii Palmer, Nuttall) and Summer Red maple (Acer rubrum L. ‘Summer Red’) liners. Light transmitted through the colored shade cloth had no influence on germination of ...

  3. Willow Creek Wildlife Mitigation Project. Final environmental assessment

    International Nuclear Information System (INIS)

    1995-04-01

    Today's notice announces BPA's proposal to fund land acquisition or acquisition of a conservation easement and a wildlife management plan to protect and enhance wildlife habitat at the Willow Creek Natural Area in Eugene, Oregon. This action would provide partial mitigation for wildlife and wildlife habitat lost by the development of Federal hydroelectric projects in the Willamette River Basin. The project is consistent with BPA's obligations under provisions of the Pacific Northwest Electric Power Planning and Conservation Act of 1980 as outlined by the Northwest Power Planning Council's 1994 Columbia River Basin Fish and Wildlife Program. BPA has prepared an environmental assessment (DOE/EA-1023) evaluating the proposed project. Based on the analysis in the EA, BPA has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an environmental impact statement (EIS) is not required and BPA is issuing this FONSI

  4. Biomass yield and fuel characteristics of short-rotation coppice (willow, poplar, empress tree)

    Energy Technology Data Exchange (ETDEWEB)

    Maier, J.; Vetter, R. [Institute for Land Management Compatible to Environmental Requirements, Muellheim (Germany)

    2004-07-01

    In two pedo-climatic different regions in the state of Baden-Wuerttemberg three shortrotation coppices willow, poplar and empress tree were tested with regard to their biomass productivity on arable land and to their properties for energetic use. Between 8 and 13 tons of dry matter per hectare and year could be produced under extensive cultivation conditions, over 15 tons with irrigation. Due to their composition, it can be assumed that their use as solid fuel in a biomass combustor is just as unproblematic as with forest timber. (orig.)

  5. Influence of Ca/Mg ratio on phytoextraction properties of Salix viminalis. II. Secretion of low molecular weight organic acids to the rhizosphere.

    Science.gov (United States)

    Magdziak, Z; Kozlowska, M; Kaczmarek, Z; Mleczek, M; Chadzinikolau, T; Drzewiecka, K; Golinski, P

    2011-01-01

    A hydroponic experiment in a phytotron was performed to investigate the effect of two different Ca/Mg ratios (4:1 and 1:10) and trace element ions (Cd, Cu, Pb and Zn) in solution on the efficiency of low molecular weight organic acid (LMWOA) formation in Salix viminalis rhizosphere. Depending on the Ca/Mg ratio and presence of selected trace elements at 0.5mM concentration, the amount and kind of LMWOAs in the rhizosphere were significantly affected. In physiological 4:1 Ca/Mg ratio the following complex of acids was observed: malonic (Pb, Zn), citric, lactic, maleic and succinic (Zn) acids. Under 1:10 Ca/Mg ratio, citric (Cd, Zn), maleic and succinic (Cd, Cu, Pb, Zn) acids were seen. Additionally, high accumulation of zinc and copper in all systems was observed, with the exception of those where one of the metals was at higher concentration. Summing up, the results indicate a significant role of LMWOAs in Salix phytoremediation abilities. Both effects can be modulated depending on the mutual Ca/Mg ratio. Copyright © 2010 Elsevier Inc. All rights reserved.

  6. Produção de frutanos em calos e plântulas clonadas in vitro de Viguiera discolor Baker (Asteraceae Fructan production in callus and in vitro cloned seedlings of Viguiera discolor Baker (Asteraceae

    Directory of Open Access Journals (Sweden)

    Nair Massumi Itaya

    2005-09-01

    Full Text Available Viguiera discolor Baker é uma espécie nativa do cerrado, cuja sobrevivência está ameaçada pela invasão de espécies exóticas. Considerando sua elevada produção e a vasta aplicação de frutanos, o presente trabalho foi conduzido visando à obtenção in vitro dessa espécie e à prospecção desses carboidratos nessas condições. Sementes foram germinadas in vitro, em meio MS modificado, e após cinco semanas de incubação, nós caulinares foram isolados e incubados no mesmo meio adicionado de 0,5 mg L-1 de ANA, regenerando plantas uniformes, raízesnão espessadas, raízes tuberosas e estruturas semelhantes a calo (calo tipo1, formadas na região caulinar basal. Análise desse material evidenciou a presença de frutanos do tipo inulina nas raízes tuberosas e nos calos tipo 1. Na presença de 2,4-D obteve-se a formação de calos friáveis (calo tipo 2, nos quais também foram detectados frutanos e suas enzimas de síntese sacarose: sacarose 1-frutosiltransferase (SST e frutano: frutano 1-frutosiltransferase (FFT. Embora em concentrações menores às observadas nas plantas cultivadas sob condições naturais, o material produzido in vitro apresentou frutanos do mesmo tipo e razão SST/FFT menor do que um. Em meio de cultura sem hormônios, foi verificada a regeneração de 50% de plantas a partir dos nós caulinares. A propagação de V. discolor in vitro pode viabilizar a multiplicação e a preservação da espécie, bem como a produção de frutanos nessas condições.Viguiera discolor Baker is a herbaceous species, native to cerrado and its survival has been threatened by the invasion of exotic species. Considering its high production and the wide application of fructans, the present work has aimed to establish in vitro culture of this species and to investigate the presence of fructans under these conditions. Seeds were germinated in vitro on modified MS medium and after plant growth, stem nodes were isolated and incubated on

  7. Results from software based empirical models of and standing biomass for poplar and willow grown as short rotation coppice

    Energy Technology Data Exchange (ETDEWEB)

    Baldwin, M.E.; Morgan, G.W.; Brewer, A.C. (Forest Research Biometrics, Surveys and Statistics Division, Forest Research, Wrecclesham (United Kingdom))

    2007-07-01

    Statistical analysis was used to create a model for estimating the quantity of biomass produced by crops of poplar and willow grown as short rotation coppice. This model was converted into a software system as described here. The software is currently available for scientific demonstration. (orig.)

  8. Biomonitoring polycyclic aromatic hydrocarbons by Salix matsudana leaves: A comparison with the relevant air content and evaluation of environmental parameter effects

    Science.gov (United States)

    Zhao, Xiangai; He, Miao; Shang, Haibo; Yu, Hongling; Wang, Hao; Li, Huijie; Piao, Jingyi; Quinto, Maurizio; Li, Donghao

    2018-05-01

    Studies on seasonal distribution characteristics of polycyclic aromatic hydrocarbons (PAHs) in Salix matsudana leaves covering its annual life cycle were carried out in order to evaluate plant leaf response sensitivity to air pollution. Salix matsudana leaves were collected throughout different development phases of plant leaf inclusive of bud break to fallen leaves, covering from spring (May) to autumn (November). Simultaneously, particle and gas samples were collected using a high volume air sampler. Seven different PAHs were determined simultaneously in these samples. The temperature dependence of the partitioning of PAHs in air and plant leaves was investigated and the results were incorporated into a mathematical model. The measured plant/air partition coefficients have been found to be exponentially proportional to the reciprocal temperature, in agreement with theoretical expectations. Furthermore, in order to define the influence of different parameters on PAH adsorption on plant leaves, area and lipid leaf content were also measured. Results demonstrated that temperature plays a very important role in PAHs partitioning and that this value should be carefully considered during sampling, in order to obtain the best correlation between PAHs concentration in air and leaves.

  9. Clinical evaluation of the effect of a herbal compound made for treatment of discolorations caused by dental fluorosis

    Directory of Open Access Journals (Sweden)

    Mahshid Mohammdi Basir

    2013-08-01

    Full Text Available   Background and Aims: The purpose of this study was research on a new herbal compound (Seidlitzia Rosmarinus made by Traditional Medicine Research Group, University of shahed to find a safer alternative to HCL-Pumice compound technique.   Materials and Methods: In this randomized clinical trial seventy two anterior teeth from 9 patients were divided in to three groups according to fluorosis severity: mild (34 teeth, moderate (14 teeth, and sever (24 teeth. In each patient, half of the teeth were treated with “Shahed” compound and other half treated with HCL-Pumice compound. Before and after treatment, photographs were taken in both groups. HCL-Pumice treatment compound was applied for 30 seconds periods and continued for 10 minutes if necessary. In case of herbal Shahed compound the time was determined by clinical symptoms or when labial contour was dismissed, If the result was not esthetistically acceptabale for the patient, HCL-Pumice compound was applied on teeth. NaF was applied after mouth washing. The photographs of the teeth before and after treatment were reviewed by two experienced observer unaware of the treatment modality. The results were analyzed using willcoxon’s, kruskal-wallis and scheffe test.   Results: There was over 81.3% acceptance between two observers and no significant differences in intraobservers evaluation (P>0.05. Improvement in beauty indexes were observed in all degrees of dental fluorosis by 18% with HCL-Pumice compound application, but “Shahed” herbal compound induced significant reduction in the amount of white spots in mild fluorosis and stain intensity of moderate fluorosis (P<0.05 , while the reduction in the severity of discoloration in group 2, these two techniques were statistically equivalent but in the remainder, HCL-Pumice compound was more significantly effective (P<0.05 .   Conclusion: HCL-Pumice compound reduces the severity of the discoloration of the teeth. Shahed herbal compound

  10. Potential for water salvage by removal of non-native woody vegetation from dryland river systems

    Science.gov (United States)

    Doody, T.M.; Nagler, P.L.; Glenn, E.P.; Moore, G.W.; Morino, K.; Hultine, K.R.; Benyon, R.G.

    2011-01-01

    Globally, expansion of non-native woody vegetation across floodplains has raised concern of increased evapotranspiration (ET) water loss with consequent reduced river flows and groundwater supplies. Water salvage programs, established to meet water supply demands by removing introduced species, show little documented evidence of program effectiveness. We use two case studies in the USA and Australia to illustrate factors that contribute to water salvage feasibility for a given ecological setting. In the USA, saltcedar (Tamarix spp.) has become widespread on western rivers, with water salvage programs attempted over a 50-year period. Some studies document riparian transpiration or ET reduction after saltcedar removal, but detectable increases in river base flow are not conclusively shown. Furthermore, measurements of riparian vegetation ET in natural settings show saltcedar ET overlaps the range measured for native riparian species, thereby constraining the possibility of water salvage by replacing saltcedar with native vegetation. In Australia, introduced willows (Salix spp.) have become widespread in riparian systems in the Murray-Darling Basin. Although large-scale removal projects have been undertaken, no attempts have been made to quantify increases in base flows. Recent studies of ET indicate that willows growing in permanently inundated stream beds have high transpiration rates, indicating water savings could be achieved from removal. In contrast, native Eucalyptus trees and willows growing on stream banks show similar ET rates with no net water salvage from replacing willows with native trees. We conclude that water salvage feasibility is highly dependent on the ecohydrological setting in which the non-native trees occur. We provide an overview of conditions favorable to water salvage. Copyright ?? 2011 John Wiley & Sons, Ltd.

  11. 3. Rethinking Borders in Empire and Nation at the Foot of the Willow Palisade

    OpenAIRE

    E.Bulag, Uradyn

    2015-01-01

    Prologue: stony wars at the foot of the willow palisade Every year, on the fifth day of the fifth lunar month, i.e. the traditional Duanwu Festival (also known as Dragon Boat Festival or Double Fifth Festival), people in Wangsiyingzi and the neighbouring village Sifangtai, just about one and half kilometres to the south, would climb atop a small mountain that lies between the two villages. Instead of racing dragon-headed boats as is the practice in south China, where the tradition first start...

  12. Willow Creek Wildlife Mitigation Project. Final Environmental Assessment.

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-04-01

    Today`s notice announces BPA`s proposal to fund land acquisition or acquisition of a conservation easement and a wildlife management plan to protect and enhance wildlife habitat at the Willow Creek Natural Area in Eugene, Oregon. This action would provide partial mitigation for wildlife and wildlife habitat lost by the development of Federal hydroelectric projects in the Willamette River Basin. The project is consistent with BPA`s obligations under provisions of the Pacific Northwest Electric Power Planning and Conservation Act of 1980 as outlined by the Northwest Power Planning Council`s 1994 Columbia River Basin Fish and Wildlife Program. BPA has prepared an environmental assessment (DOE/EA-1023) evaluating the proposed project. Based on the analysis in the EA, BPA has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an environmental impact statement (EIS) is not required and BPA is issuing this FONSI.

  13. Baseline element concentrations in soils and plants, Wattenmeer National Park, North and East Frisian Islands, Federal Republic of Germany

    Science.gov (United States)

    Severson, R.C.; Gough, L.P.; van den Boom, G.

    1992-01-01

    Baseline element concentrations are given for dune grass (Ammophilia arenaria), willow (Salix repens), moss (Hylocomium splendens) and associated surface soils. Baseline and variability data for pH, ash, Al, As, Ba, C, Ca, Cd, Ce, Co, Cr, Cu, Fe, Hg, K, La, Li, Mg, Mn, Na, Nb, Nd, Ni, P, Pb, S, Sc, Se, Sr, Th, Ti, V, Y, Yb, and Zn are reported; however, not all variables are reported for all media because, in some media, certain elements were below the analytical detection limit. Spatial variation in element concentration between five Frisian Islands are given for each of the sample media. In general, only a few elements in each media showed statistically significant differences between the islands sampled. The measured concentrations in all sample media exhibited ranges that cannot be attributed to anthropogenic additions of trace elements, with the possible exception of Hg and Pb in surface soils.Baseline element concentrations are given for dune grass (Ammophilia arenaria), willow (Salix repens), moss (Hylocomium splendens) and associated surface soils. Baseline and variability data for pH, ash, Al, As, Ba, C, Ca, Cd, Ce, Co, Cr, Cu, Fe, Hg, K, La, Li, Mg, Mn, Na, Nb, Nd, Ni, P, Pb, S, Sc, Se, Sr, Th, Ti, V, Y, Yb, and Zn are reported; however, not all variables are reported for all media because, in some media, certain elements were below the analytical detection limit. Spatial variation in element concentration between five Frisian Islands are given for each of the sample media. In general, only a few elements in each media showed statistically significant differences between the islands sampled. The measured concentrations in all sample media exhibited ranges that cannot be attributed to anthropogenic additions of trace elements, with the possible exception of Hg and Pb in surface soils.

  14. Factors Affecting Diet Variation in the Pyrenean Rock Ptarmigan (Lagopus muta pyrenaica: Conservation Implications.

    Directory of Open Access Journals (Sweden)

    Ricardo García-González

    Full Text Available The Pyrenean rock ptarmigan (Lagopus muta pyrenaica lives at one of the southernmost limits of the ptarmigan range. Their small population sizes and the impacts of global changes are limiting factors in the conservation of this threatened subspecies. An effective conservation policy requires precise basic knowledge of a species' food and habitat requirements, information that is practically non-existent for this Pyrenean population. Here, we describe the diet of a ptarmigan population in the Eastern Pyrenees, the environmental factors influencing its variability and the relationship between diet floristic composition and quality. Diet composition was determined by microhistological analysis of faeces and diet quality was estimated from free-urate faecal N content. Our results show that grouse diet is based mainly on arctic-alpine shrubs of the Ericaceae family, as well as dwarf willows (Salix spp. and Dryas octopetala. The most frequently consumed plant species was Rhododendron ferrugineum, but its abundance in the diet was negatively related to the diet nitrogen content. Conversely, the abundance of Salix spp., grass leaves and arthropods increased the nitrogen content of the diet. Seasonality associated with snow-melting contributed the most to variability in the Pyrenean ptarmigan diet, differentiating winter from spring/summer diets. The latter was characterised by a high consumption of dwarf willows, flowers, arthropods and tender forb leaves. Geographic area and sex-age class influenced diet variability to a lesser extent. Current temperature increases in the Pyrenees due to global warming may reduce the persistence and surface area of snow-packs where preferred plants for rock ptarmigan usually grow, thus reducing food availability. The high consumption of Rh. ferrugineum characterised the diet of the Pyrenean population. Given the toxicity of this plant for most herbivores, its potential negative effect on Pyrenean ptarmigan populations

  15. Semi-continuous anaerobic co-digestion of cow manure and steam-exploded Salix with recirculation of liquid digestate.

    Science.gov (United States)

    Estevez, Maria M; Sapci, Zehra; Linjordet, Roar; Schnürer, Anna; Morken, John

    2014-04-01

    The effects of recirculating the liquid fraction of the digestate during mesophilic anaerobic co-digestion of steam-exploded Salix and cow manure were investigated in laboratory-scale continuously stirred tank reactors. An average organic loading rate of 2.6 g VS L(-1) d(-1) and a hydraulic retention time (HRT) of 30 days were employed. Co-digestion of Salix and manure gave better methane yields than digestion of manure alone. Also, a 16% increase in the methane yield was achieved when digestate was recirculated and used instead of water to dilute the feedstock (1:1 dilution ratio). The reactor in which the larger fraction of digestate was recirculated (1:3 dilution ratio) gave the highest methane yields. Ammonia and volatile fatty acids did not reach inhibitory levels, and some potentially inhibitory compounds released during steam explosion (i.e., furfural and 5-hydroxy methyl furfural) were only detected at trace levels throughout the entire study period. However, accumulation of solids, which was more pronounced in the recycling reactors, led to decreased methane yields in those systems after three HRTs. Refraining from the use of fresh water to dilute biomass with a high-solids content and obtaining a final digestate with increased dry matter content might offer important economic benefits in full-scale processes. To ensure long-term stability in such an approach, it would be necessary to optimize separation of the fraction of digestate to be recirculated and also perform proper monitoring to avoid accumulation of solids. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. The silviculture, nutrition and economics of short rotation willow coppice in the uplands of mid-Wales

    Energy Technology Data Exchange (ETDEWEB)

    Heaton, R J; Randerson, P F; Slater, F M

    2000-07-01

    The potential of short rotation coppice as a biomass crop on land over 250m (the uplands) of mid Wales was studied. The results found in this study indicate that growing short rotation coppice willow in the uplands is a viable proposition with regard to establishment success and yields. In the event of a secure wood chip market in Wales, returns to the grower would be comparable to those from sheep production. (author)

  17. Uptake and activity concentration of 137Cs and 90Sr in Salix viminalis

    International Nuclear Information System (INIS)

    Rosen, K.; Fircks, Y. von; Sennerby-Forsse, L.

    1999-01-01

    In order to gain a better understanding of the uptake, internal distribution of 137 Cs and 90 Sr and the effects of K-fertilisation of uptake of 137 Cs in Salix plantations, a K-fertilisation experiment including 137 Cs, 90 Sr and 40 K was performed. Previous studies with K-fertilization in Sweden have mainly dealt with the flow of 137 Cs between soil to plant in annual crops. The aim of this study was study the transfer of 137 Cs, 90 Sr and 40 K from soil to plants and to test the following hypothesis: that accumulation of 137 Cs and 90 Sr differs between plant organs, that there is a seasonal variation in uptake and concentration of 137 Cs and 90 Sr and that the availability of K is related to the uptake of 137 Cs. (au)

  18. Evaluation of the effectiveness of lamination for preventing discoloration and fading of radiation warning signs posted on the exterior of radiation facilities

    International Nuclear Information System (INIS)

    Hiroi, Tomoko; Ootaki, Masanori; Nawa, Yukino; Kuwabara, Rie; Tatsunami, Shinobu; Matsui, Hiroaki; Kumazawa, Yutaka; Yamamoto, Takio

    2014-01-01

    Radiation warning signs posted on the exterior of radiation facilities become faded and discolored with time. There are various types of commercially available laminating films for protecting signs from ultraviolet light. We examined the protection effect of polyvinyl chloride (PVC), acrylic resin and fluororesin films applied to the surface of radiation warning signs. The laminated signs were exposed to direct sunlight on the wall of an air filter chamber of a radiation facility for 1200 days. Simultaneously, another set of laminated signs was exposed to light from a xenon-arc weatherometer for 1200 hours. After exposure, the colors on the surface of each sign were evaluated digitally by using a spectrum colorimeter. The results indicated that lamination with a film that blocks ultraviolet light is effective for protecting the signs from fading and discoloration. For long-term protection under direct sunlight, PVC was the most effective among the three materials tested. (author)

  19. Vegetation and flora of short-rotation willow stands from a conservation viewpoint

    Energy Technology Data Exchange (ETDEWEB)

    Gustafsson, L.

    1986-01-01

    The energy forests were studied using random 0.5 x 0.5 m quadrats in which cover for field- and bottom-layer species was recorded in a percentage scale. The data were analysed using various multivariate methods. The vegetation and flora of twelve coppices in southern Sweden were investigated. Also the succession during the establishment phase on a meadow on the west coast and on a peat bog in the east-central part of the country was studied. Moreover, the impact on fen vegetation downstream of an energy forest on a peat bog was investigated. The flora and vegetation of energy forests is dominated by common ruderal species like Cirsium arvense, Galeopsis tetrahit and Urtica dioica. Uncommon species from woodland and fen habitats are only occasionally found. The field layer of energy forests shows a clear seasonality with maximal cover in July. The species number and the composition of vegetation vary greatly between the coppices and is largely determined by the management system. Intense cultivation including fertilization and the use of herbicides depletes the flora. Long rotation times decrease both species diversity and the field-layer cover. Uncommon species are mostly found in old coppices, which also often have a rich spring flora. A change in flora and vegetation occurs when an energy forest is established. The change is great and the time needed for stabilization of the energy forest flora is long when the original flora is dissimilar to the coppice flora. Willow coppices can increase habitat diversity in regions dominated by coniferous forests or in large-scale agricultural areas. Structural heterogeneity of a stand promotes diversity of the flora. Such heterogeneity can be created if a stand is composed of a variety of willow clones, if it is harvested at intervals, contains gaps, open ditches and is surrounded by edge zones of various types.

  20. Detectability of landscape effects on recolonization increases with regional population density.

    Science.gov (United States)

    Liman, Anna-Sara; Dalin, Peter; Björkman, Christer

    2015-07-01

    Variation in population size over time can influence our ability to identify landscape-moderated differences in community assembly. To date, however, most studies at the landscape scale only cover snapshots in time, thereby overlooking the temporal dynamics of populations and communities. In this paper, we present data that illustrate how temporal variation in population density at a regional scale can influence landscape-moderated variation in recolonization and population buildup in disturbed habitat patches. Four common insect species, two omnivores and two herbivores, were monitored over 8 years in 10 willow short-rotation coppice bio-energy stands with a four-year disturbance regime (coppice cycle). The population densities in these regularly disturbed stands were compared to densities in 17 undisturbed natural Salix cinerea (grey willow) stands in the same region. A time series approach was used, utilizing the natural variation between years to statistically model recolonization as a function of landscape composition under two different levels of regional density. Landscape composition, i.e. relative amount of forest vs. open agricultural habitats, largely determined the density of re-colonizing populations following willow coppicing in three of the four species. However, the impact of landscape composition was not detectable in years with low regional density. Our results illustrate that landscape-moderated recolonization can change over time and that considering the temporal dynamics of populations may be crucial when designing and evaluating studies at landscape level.

  1. Soil physical and hydrological properties under three biofuel crops in Ohio

    Energy Technology Data Exchange (ETDEWEB)

    Bonin, Catherine; Lal, Rattan [The Ohio State Univ., School of Environment and Natural Resources, Carbon Management and Sequestration Center, Columbus, OH (United States); Schmitz, Matthias [Rheinische Friedrich/Wilhelms-Universitaet Bonn, Steinmann Institut fuer Geologie, Mineralogie und Palaeontologie, Bonn (Germany); Wullschleger, S. [The Oakridge National Lab., Oakridge, TN (United States)

    2012-10-15

    While biofuel crops are widely studied and compared for their energy and carbon footprints, less is known about their effects on other soil properties, particularly hydrologic characteristics. Soils under three biofuel crops, corn (Zea mays), switchgrass (Panicum virgatum), and willow (Salix spp.), were analyzed seven years after establishment to assess the effects on soil bulk density ({rho}{sub b}), penetration resistance (PR), water-holding capacity, and infiltration characteristics. The PR was the highest under corn, along with the lowest associated water content, while PR was 50-60 % lower under switchgrass. In accordance with PR data, surface (0-10 cm) bulk density also tended to be lower under switchgrass. Both water infiltration rates and cumulative infiltration amounts varied widely among and within the three crops. Because the Philip model did not fit the data, results were analyzed using the Kostiakov model instead. Switchgrass plots had an average cumulative infiltration of 69 cm over 3 hours with a constant infiltration rate of 0.28 cm min{sup -1}, compared with 37 cm and 0.11 cm min{sup -1} for corn, and 26 cm and 0.06 cm min{sup -1} for willow, respectively. Results suggest that significant changes in soil physical and hydrologic properties may require more time to develop. Soils under switchgrass may have lower surface bulk density, higher field water capacity, and a more rapid water infiltration rate than those under corn or willow.

  2. Soil physical and hydrological properties under three biofuel crops in Ohio

    Energy Technology Data Exchange (ETDEWEB)

    Bonin, Catherine [Ohio State University; Lal, Dr. Rattan [Ohio State University; Schmitz, Matthias [Rheinsche Friedrich/Wilhelms Universitaet Boon; Wullschleger, Stan D [ORNL

    2012-01-01

    While biofuel crops are widely studied and compared for their energy and carbon footprints, less is known about their effects on other soil properties, particularly hydrologic characteristics. Soils under three biofuel crops, corn (Zea mays), switchgrass (Panicum virgatum), and willow (Salix spp.), were analyzed seven years after establishment to assess the effects on soil bulk density ({rho}{sub b}), penetration resistance (PR), water-holding capacity, and infiltration characteristics. The PR was the highest under corn, along with the lowest associated water content, while PR was 50-60% lower under switchgrass. In accordance with PR data, surface (0-10 cm) bulk density also tended to be lower under switchgrass. Both water infiltration rates and cumulative infiltration amounts varied widely among and within the three crops. Because the Philip model did not fit the data, results were analyzed using the Kostiakov model instead. Switchgrass plots had an average cumulative infiltration of 69 cm over 3 hours with a constant infiltration rate of 0.28 cm min{sup -1}, compared with 37 cm and 0.11 cm min{sup -1} for corn, and 26 cm and 0.06 cm min{sup -1} for willow, respectively. Results suggest that significant changes in soil physical and hydrologic properties may require more time to develop. Soils under switchgrass may have lower surface bulk density, higher field water capacity, and a more rapid water infiltration rate than those under corn or willow.

  3. The effect of water availability on stand-level productivity, transpiration, water use efficiency and radiation use efficiency of field-grown willow clones

    DEFF Research Database (Denmark)

    Linderson, Maj-Lena; Iritz, Z.; Lindroth, A.

    2007-01-01

    The effect of water availability on stand-level productivity, transpiration, water use efficiency (WUE) and radiation use efficiency (RUE) is evaluated for different willow clones at stand level. The measurements were made during the growing season 2000 in a 3-year-old plantation in Scania......, southernmost Sweden. Six willow clones were included in the study: L78183, SW Rapp, SW Jorunn, SW Jorr, SW Tora and SW Loden. All clones were exposed to two water treatments: rain-fed, non-irrigated treatment and reduced water availability by reduced soil water recharge. Field measurements of stem sap...... low compared to other results. Generally, all clones, except for Jorunn, seem to be better off concerning biomass production, WUE and RUE than the reference clone. Jorr, Jorunn and Loden also seem to be able to cope with the reduced water availability with increase in the water use efficiency. Tora...

  4. Salix response to different flow regimes in controlled experiments: first results

    Science.gov (United States)

    Gorla, Lorenzo; Signarbieux, Constant; Buttler, Alexandre; Perona, Paolo

    2013-04-01

    Dams and water management for hydropower production, agriculture and other human activities alter the natural flow regime of rivers. The new river hydrograph components depend on the type of impoundment and the policy of regulation but such a different flow regime will likely affect the riparian environment. The main challenge in order to define sustainable flow releases is to quantify hydrological effects in terms of geomorphology and ecosystem response. A considerable lack of knowledge still affects the link hydrology-ecology and inadequate flow rules (e.g., minimal or residual flows) are consequently still widespread: further research in this direction is urgently required. We present an experiment, which aims to investigate the effects of different water stage regimes on riparian vegetation (salix Viminalis cuttings) development in a temperate region (Switzerland). This work describes the installation setup, together with the first results concerning the first of the two scheduled seasons of campaign. Sixty Salix cuttings were planted in non-cohesive sandy-gravel sediment within 1 meter tall plastic pots installed outside in the EPFL campus. After grouping them in three batteries, the water level within them has been varying following three river regimes simulated by adjusting the water level within the pots by means of an automatic hydraulic system. The three water level regimes reproduce a natural flow regime, a minimum residual flow policy, which only conserves peaks during flooding conditions, and an artificial regime conserving only low frequencies (e.g., seasonality) of the natural dynamic. The natural flow regime of the first battery has been applied for two months to the entire system; the three regimes above said started in June 2012. This triggered a plant response transitory regime, which we monitored by measuring plant growth, soil and atmospheric variables. Particularly, measures concern with branches development leaves photosynthesis and

  5. Salix daphnoides: A Screening for Oligomeric and Polymeric Proanthocyanidins

    Directory of Open Access Journals (Sweden)

    Stefan Wiesneth

    2015-07-01

    Full Text Available In the present study, a qualitative analysis of proanthocyanidins (PAs from an aqueous-methanolic extract of Salix daphnoides VILL. bark is described. Procyanidin B1 (1, B2 (2, B3 (3, B4 (4, C1 (5, epicatechin-(4β→8-epicatechin-(4β→8-catechin (6 and epicatechin-(4β→8-epicatechin-(4β→8-epicatechin-(4β→8-catechin (7 have been isolated by a combination of different chromatographic separations on Sephadex® LH-20-, MCI®-, Diol-and RP-18-phases. Mass spectrometry, 1D- and 2D-NMR, circular dichroism and polarimetry were used for their structure elucidation and verification by comparison with the literature. Additionally, two fractions of very polar flavan-3-ols were compared: “regular” polymeric PAs received at the very end of the Sephadex® LH-20 chromatography showing no mobility on silica TLC and “unusual” PAs with the same RF-value but already eluting together with flavonoids in the Sephadex® LH-20 system. These “unusual” PAs were subsequently enriched by centrifugal partition chromatography (CPC. 13C-NMR, polarimetry, thiolysis, acid hydrolysis and phloroglucinol degradation were used to characterize both fractions. Differences in the composition of different flavan-3-ol units and the middle chain length were observed.

  6. Protective effect of porphyran isolated from discolored nori (Porphyra yezoensis) on lipopolysaccharide-induced endotoxin shock in mice.

    Science.gov (United States)

    Nishiguchi, Tomoki; Cho, Kichul; Isaka, Shogo; Ueno, Mikinori; Jin, Jun-O; Yamaguchi, Kenichi; Kim, Daekyung; Oda, Tatsuya

    2016-12-01

    Porphyran, a sulfated polysaccharide, isolated from discolored nori (Porphyra yezoensis) (dc-porphyran) and one fraction (F1) purified from dc-porphyran by DEAE-chromatography showed the protective effects on LPS-induced endotoxin shock in mice. Intraperitoneal (i.p.) treatment with dc-porphyran or F1 (100mg/kg) 60min prior to i.p. injection of LPS (30mg/kg) completely protected mice from LPS lethality. At 10mg/kg concentration, F1 demonstrated more protection than dc-porphyran. Intravenous (i.v.) challenge of LPS, even at 20mg/kg, was more lethal than i.p. administration; i.v. injection of F1 (100mg/kg) with LPS significantly improved the survival rate. However, i.v. dc-porphyran (100mg/kg) produced an even lower survival rate than that of LPS alone. We examined pro-inflammatory mediators such as NO and TNF-α in serum. F1 significantly reduced the levels of these markers. Additionally, F1 significantly decreased the malondialdehyde level in the liver, a marker of oxidative stress, while dc-porphyran had almost no effect. Furthermore, F1 significantly decreased the production of TNF-α and NO in peritoneal exudate cells harvested from LPS-challenged mice, while dc-porphyran treatment showed a lesser decrease. Our results suggest that porphyran isolated from discolored nori, especially F1, is capable of suppressing LPS-induced endotoxin shock in vivo. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Removal of 4-chlorobenzoic acid from spiked hydroponic solution by willow trees (Salix viminalis)

    Czech Academy of Sciences Publication Activity Database

    Deavers, K.; Macek, Tomáš; Karlson, U. G.; Trapp, S.

    2010-01-01

    Roč. 17, č. 7 (2010), s. 1355-1361 ISSN 0944-1344 R&D Projects: GA ČR GA203/06/0563 Institutional research plan: CEZ:AV0Z40550506 Keywords : chlorobenzoic acid * degradation * metabolism * PCB Subject RIV: EI - Biotechnology ; Bionics Impact factor: 2.870, year: 2010

  8. Bioengineering applied to erosion and stability control in the North Apennines (Emilia-Romagna Region, Italy): a check about critical aspects of the works.

    Science.gov (United States)

    Selli, Lavinia; Cavazza, Claudio; Pavanelli, Donatella

    2013-04-01

    Because of its geological structure, in the Emilia-Romagna Region over 32,000 landslides have been identified. Several works have been made in order to control mass movement's dynamics and to secure of Reno and Lamone Mountain Basin Rivers, the road network and near by villages and towns. Most of the control works dealt with bioengineering practices: palisades piles, geotextiles, seedings, surface flow control works, dikes within main drainage ditches. In order to check about critical aspects related to the use of these techniques in the Apennines, a survey in this basins was designed with specific interest in the several kinds of works realised, in which plant species were mostly used and in the factors that affected the success or failure of the works. Territory encompasses steep slopes covered with woods to low reliefs covered with grasslands. It is characterized by prevailing clays, inducing instability, and arenaceous lithology with impermeable soils; drainage density is quite high and hillsides suffer extensive and severe erosion and slope stability problems. Chestnut woods mainly represent land use at higher altitudes, while coppice, pastures and crops are present on milder hillsides. The remaining part of the basin is covered by vineyards, orchards, ponds and urban areas, which are basically located in the valley floor. Precipitation events mainly consist of rainfall ranging between 950-1015 mm per year; few snowfalls occur during winter and a long dry season lasts from June until September. We have analyzed 187 works designed mainly for the consolidation of slope instabilities through a widespread enhancement of the vegetation cover. The surveyed works are classified as a function of their building features: it can be seen that cribwalls and palisades are by far the most common types, being the 24% and the 34% respectively of the works. As far as the most adopted plant species, they were silver willow (Salix alba), Spanish Broom (Spartium Junceum) and

  9. The influence of electrical charge and indolebutyric acid on rooting of willow cuttings

    Directory of Open Access Journals (Sweden)

    R. Domański

    2015-06-01

    Full Text Available Willow cuttings were treated by direct electrical current (DEC, with a negative or a positive electrode inserted inside them, and with the other being outside. Both directions of DEC flow between electrodes stimulated rooting as actively as growth stimulators. The mung bean or oat coleoptile straight growth test showed that the cuttings released some growth promoters into the surrounding water. However, the simultaneous release of some undetected rooting inhibitors was not excluded. The experiment, shows the possibility of changing the chemical regulatory mechanisms of tissues by the use of weak, direct electrical current which causes, the migration of particular substance{s into or from a definite tissue or organ.

  10. Effects of ambient versus reduced UV-B radiation on high arctic ¤Salix arctica¤ assessed by measurements and calculations of chlorophyll a fluorescence parameters from fluorescence transients

    DEFF Research Database (Denmark)

    Albert, K.R.; Mikkelsen, Teis Nørgaard; Ro-Poulsen, H.

    2005-01-01

    A UV-B exclusion-experiment was conducted in the high arctic Zackenberg, NE Greenland, in which Salix arctica leaves during most of the growing season were fixed perpendicular to the solar zenith angle, thereby receiving maximal solar radiation. Covered with Teflon and Mylar foil, the leaves...

  11. Ambient UV-B radiation reduces PSII performance and net photosynthesis in high Arctic Salix arctica

    DEFF Research Database (Denmark)

    Albert, Kristian Rost; Mikkelsen, Teis Nørgaard; Ro-Poulsen, Helge

    2011-01-01

    Ambient ultraviolet-B (UV-B) radiation potentially impacts the photosynthetic performance of high Arctic plants. We conducted an UV-B exclusion experiment in a dwarf shrub heath in NE Greenland (74°N), with open control, filter control, UV-B filtering and UV-AB filtering, all in combination......, nitrogen and UV-B absorbing compounds. Compared to a 60% reduced UV-B irradiance, the ambient solar UV-B reduced net photosynthesis in Salix arctica leaves fixed in the 45° position which exposed leaves to maximum natural irradiance. Also a reduced Calvin Cycle capacity was found, i.e. the maximum rate...... across position in the vegetation. These findings add to the evidence that the ambient solar UV-B currently is a significant stress factor for plants in high Arctic Greenland....

  12. Aporphine alkaloids with antitubercular activity isolated from Ocotea discolor Kunth (Lauraceae

    Directory of Open Access Journals (Sweden)

    Monica Constanza Avila Murillo

    2017-09-01

    Full Text Available Tuberculosis disease causes thousands of deaths worldwide and, currently, the used drugs are either not enough or obsolete for its treatment. Therefore, new compounds that combat this disease are been seek. Thus, the antituberculosis activity of the alkaloids ocoxilonine (1, ocoteine (2, dicentrine (3 and 1,2-methylenedioxy-3,10,11-trimethoxyaporphine (4, isolated from Ocotea discolor wood was evaluated. Their structures were identified by analysis of nuclear magnetic resonance spectroscopic data (NMR 1D – 1H, 13C, 2D – COSY, HSQC and HMBC, mass spectra, and comparison with literature data. All the isolated compounds demonstrated antituberculosis activity, with ocoteine (2 being the most active compound, with a minimum inhibitory concentration value of 140 μM against the virulent strain Mycobacterium tuberculosis H37Rv. All the isolated compounds showed antituberculosis activity, with a variation range in the minimum inhibitory concentration between 140 to 310 μM, being ocoteine (2 the most active compound against the virulent strain Mycobacterium tuberculosis H37Rv.

  13. Preliminary Risk Assessment of the Southwestern Willow Flycatcher (Empidonax traillii extimus) at the Los Alamos National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Gallegos, A.F.; Gonzales, G.J.; Bennett, K.D.; Mullen, M.A.; Foxx, T.S.

    1998-10-01

    The southwestern willow flycatcher (Empidonax traillii extimus) is the fourth threatened or endangered species to undergo a preliminary assessment for estimating potential risk from environmental contaminants at the Los Alamos National Laboratory. The assessments are being conducted as part of a three-year project to develop a habitat management plan for threatened and endangered species and species of concern at the Laboratory. For the preliminary assessment, estimated doses were compared against toxicity reference values to generate hazard indices (HIs). This assessment included a measure of cumulative effects from multiple contaminants (radionuclides, metals, and organic chemicals) to 100 simulated nest sites located within flycatcher potential habitat. Sources of contaminant values were 10,000-ft{sup 2} grid cells within an Ecological Exposure Unit (EEU). This EEU was estimated around the potential habitat and was based on the maximum home range for the fly catcher identified in the scientific literature. The tools used included a custom FORTRAN program, ECORSK5, and a geographic information system. Food consumption and soil ingestion contaminant pathways were addressed in the assessment. Using a four-category risk evaluation, HI results indicate no appreciable impact is expected to the southwestern willow flycatcher. Information on risk by specific geographical location was generated, which can be used to manage contaminated areas, flycatcher habitat, facility siting, and/or facility operations in order to maintain low levels of risk from contaminants.

  14. Preliminary Risk Assessment of the Southwestern Willow Flycatcher (Empidonax traillii extimus) at the Los Alamos National Laboratory

    International Nuclear Information System (INIS)

    Gallegos, A.F.; Gonzales, G.J.; Bennett, K.D.; Mullen, M.A.; Foxx, T.S.

    1998-01-01

    The southwestern willow flycatcher (Empidonax traillii extimus) is the fourth threatened or endangered species to undergo a preliminary assessment for estimating potential risk from environmental contaminants at the Los Alamos National Laboratory. The assessments are being conducted as part of a three-year project to develop a habitat management plan for threatened and endangered species and species of concern at the Laboratory. For the preliminary assessment, estimated doses were compared against toxicity reference values to generate hazard indices (HIs). This assessment included a measure of cumulative effects from multiple contaminants (radionuclides, metals, and organic chemicals) to 100 simulated nest sites located within flycatcher potential habitat. Sources of contaminant values were 10,000-ft 2 grid cells within an Ecological Exposure Unit (EEU). This EEU was estimated around the potential habitat and was based on the maximum home range for the fly catcher identified in the scientific literature. The tools used included a custom FORTRAN program, ECORSK5, and a geographic information system. Food consumption and soil ingestion contaminant pathways were addressed in the assessment. Using a four-category risk evaluation, HI results indicate no appreciable impact is expected to the southwestern willow flycatcher. Information on risk by specific geographical location was generated, which can be used to manage contaminated areas, flycatcher habitat, facility siting, and/or facility operations in order to maintain low levels of risk from contaminants

  15. Short-rotation coppices. State of the realizability, organisation and a model for the evaluation of the production and supply of rapidly growing wood from short-rotation coppices als a biofuel for biomass-fuelled heating power stations in Bavaria; Kurzumtriebsplantagen. Stand der Umsetzbarkeit, Organisation und ein Modell zur oekonomischen Bewertung von Produktion und Bereitstellung schnell wachsenden Holzes aus Kurzumtriebsplantagen als biogener Festbrennstoff fuer Biomasse(heiz)kraftwerke in Bayern

    Energy Technology Data Exchange (ETDEWEB)

    Paschlau, Helmut F

    2011-04-07

    The study examines most aspects of Short-rotation Coppice Crops (SRC), mainly from willows (Salix sp.) and poplars (Populus sp.), for energetic use in big biomass powerstations in Bavaria (southern Germany). In addition to the compilation of framework conditions concerning environmental and agrarian politics as well as legal issues, every link in the process chain of SRC will be considered - from planting to harvesting, treatment of the wood chips and Just-in-time delivery to the powerplant - followed by an evaluation of SRC in ecological terms. The basic aim of this study is to evaluate every single link with regard to organisational und economic issues, analysis of relevant markets and to develop a comprehensive calculation model for the amount of annuities of the whole process chain.

  16. Uptake of naturally occurring radioisotopes by vegetation in a region of high radioactivity

    Energy Technology Data Exchange (ETDEWEB)

    Mahon, D C; Mathewes, R W [Simon Fraser Univ., Burnaby, British Columbia (Canada)

    1983-05-01

    The accumulation of four naturally occurring radionuclides of the sup(238)U series, uranium, sup(226)Ra, sup(210)Pb and sup(210)Po, in 15 species of native plants was investigated. Of the plants sampled some accumulated, but none concentrated uranium or sup(226)Ra. Grouseberry (Vaccinium scoparium Leiberg) appeared to concentrate sup(210)Pb and sup(210)Po. There were marked seasonal variations in the accumulation of uranium by grass (Calamagrostis rubescens Buckl.), and of sup(210)Pb and sup(210)Po by grass and fireweed (Epilobium angustifolium L.). High positive correlations between soil and vegetation radionuclide content were found for uranium in fireweed and grouseberry in spring, sup(226)Ra in willow (Salix scouleriana L.) in spring, and uranium in fireweed in summer.

  17. Failure analysis of edge discoloration of galvanized fuel tank

    Directory of Open Access Journals (Sweden)

    Jitendra Mathur

    2015-10-01

    Full Text Available A peculiar type of edge discoloration defect on the surface of some galvanized fuel tank was observed, causing significant appearance problems. In the present study, the surface defect was characterized by visual inspection, optical microscopy, scanning electron microscopy and energy dispersive spectroscopic analysis to understand the source and mechanism of the defect. In the visual inspection, these peculiar surface appearances were observed in fuel tank at three distinct locations. The SEM examination exhibited two distinct regions on the surface apart from the normal galvanized surface: (1 galvannealed, (2 mixture of galvanized and galvannealed texture. The energy dispersive spectroscopic analysis of galvannealed region indicated enrichment of Zn and Al whereas in the region of galvanized majorly Zn was observed. Surface texture of galvannealed region showed majorly zeta crystals along with skin pass marks; whereas no such zeta crystals were observed in case of galvanized regions. Based on the preliminary results, the following hypothesis was made: Coil processed during galvanizing to galvannealing transition. Thickness and width changed to wider and thicker section, which resulted into lower line speed. Due to the lower Al content, lower speed and thicker section combination resulted in formation of partial GA in the coil owing to the internal heat content of the coil. This paper presents the results of the investigation.

  18. The effect of water availability on stand-level productivity, transpiration, water use efficiency and radiation use efficiency of field-grown willow clones

    International Nuclear Information System (INIS)

    Linderson, Maj-Lena; Iritz, Zinaida; Lindroth, Anders

    2007-01-01

    The effect of water availability on stand-level productivity, transpiration, water use efficiency (WUE) and radiation use efficiency (RUE) is evaluated for different willow clones at stand level. The measurements were made during the growing season 2000 in a 3-year-old plantation in Scania, southernmost Sweden. Six willow clones were included in the study: L78183, SW Rapp, SW Jorunn, SW Jorr, SW Tora and SW Loden. All clones were exposed to two water treatments: rain-fed, non-irrigated treatment and reduced water availability by reduced soil water recharge. Field measurements of stem sap-flow and biometry are up-scaled to stand transpiration and stand dry substance production and used to assess WUE. RUE is estimated from the ratio between the stand dry substance production and the accumulated absorbed photosynthetic active radiation over the growing season. The total stand transpiration rate for the 5 months lies between 100 and 325 mm, which is fairly low compared to the Penman-Monteith transpiration for willow, reaching 400-450 mm for the same period. Mean WUE of all clones and treatments is 5.3 g/kg, which is high compared to earlier studies, while average RUE is 0.31 g/mol, which is slightly low compared to other results. Generally, all clones, except for Jorunn, seem to be better off concerning biomass production, WUE and RUE than the reference clone. Jorr, Jorunn and Loden also seem to be able to cope with the reduced water availability with increase in the water use efficiency. Tora performs significantly better than the other clones concerning both growth and efficiency in light and water use, but the effect of the dry treatment on stem growth shows sensitivity to water availability. The reduced stem growth could be due to a change in allocation patterns

  19. Stem Photosynthesis of Twig and Its Contribution to New Organ Development in Cutting Seedlings of Salix Matsudana Koidz.

    Directory of Open Access Journals (Sweden)

    Junxiang Liu

    2018-04-01

    Full Text Available The objective of this study was to illustrate the photosynthetic characteristics of current twigs of Salix matsudana Koidz., and clarify the effect of stem photosynthesis on the new organ development in cutting seedlings. Excised twigs were taken as the experimental samples. The response of the stem photosynthesis rate to increasing light intensity and the effective photochemical efficiency of the cross section of the twig were determined. Then, twigs were used as cuttings and exposed to 0, 20, and 100 μmol m−2 s−1 light intensities, respectively, to achieve distinctive stem photosynthetic rates. After 14 days of treatment, stem water and non-structural carbohydrate (NSC content, as well as the biomass and carbon isotopic composition, of new organs in the cutting seedlings under different light treatments were examined. The results showed that the gross photosynthetic rate significantly increased within 400 μmol m−2 s−1 of light intensity, and the maximum rate was approximately 1.27 μmol m−2 s−1. The effective photochemical efficiency of the PSⅡ of the cortex was significantly higher than the inner tissues in the cross section of the twig. When twig cuttings were exposed to different light intensities, stem water and starch content, as well as bud and root biomass, were significantly higher in the cutting seedling subjected to 100 μmol m−2 s−1 than the case treated in darkness; however, the bud δ13C trend was the opposite. Stem photosynthesis played a positive role in the maintenance of stem water and starch supply for the cutting seedlings, and 13C depleted assimilates produced by stem photosynthesis contributed to bud biomass, revealing that stem photosynthesis promotes organ development in cutting seedlings of Salix matsudana.

  20. Bioenergy production from perennial energy crops: a consequential LCA of 12 bioenergy scenarios including land use changes.

    Science.gov (United States)

    Tonini, Davide; Hamelin, Lorie; Wenzel, Henrik; Astrup, Thomas

    2012-12-18

    In the endeavor of optimizing the sustainability of bioenergy production in Denmark, this consequential life cycle assessment (LCA) evaluated the environmental impacts associated with the production of heat and electricity from one hectare of Danish arable land cultivated with three perennial crops: ryegrass (Lolium perenne), willow (Salix viminalis) and Miscanthus giganteus. For each, four conversion pathways were assessed against a fossil fuel reference: (I) anaerobic co-digestion with manure, (II) gasification, (III) combustion in small-to-medium scale biomass combined heat and power (CHP) plants and IV) co-firing in large scale coal-fired CHP plants. Soil carbon changes, direct and indirect land use changes as well as uncertainty analysis (sensitivity, MonteCarlo) were included in the LCA. Results showed that global warming was the bottleneck impact, where only two scenarios, namely willow and Miscanthus co-firing, allowed for an improvement as compared with the reference (-82 and -45 t CO₂-eq. ha⁻¹, respectively). The indirect land use changes impact was quantified as 310 ± 170 t CO₂-eq. ha⁻¹, representing a paramount average of 41% of the induced greenhouse gas emissions. The uncertainty analysis confirmed the results robustness and highlighted the indirect land use changes uncertainty as the only uncertainty that can significantly change the outcome of the LCA results.

  1. Par Pond vegetation status summer 1995 - July survey descriptive summary

    International Nuclear Information System (INIS)

    Mackey, H.E. Jr.; Riley, R.S.

    1995-07-01

    A survey of the emergent shoreline aquatic plant, communities began in June 1995, three months after the refilling of Par Pond to approximately 200 feet (61 meters) above mean sea level, and continued with this July survey. Aquatic plant communities, similar to the pre-drawdown Par Pond communities, are becoming reestablished. Beds of maidencane (Panicum hemitomon), lotus (Nelumbo lutea), water lily (Nymphaea odorata), and watershield (Brasenia schreberi) are now extensive and well established. In addition, within isolated coves, extensive beds of water lilies and spike-rush (Eleocharis sp.) are common. Cattail occurrence has increased since refill, but large beds common to Par Pond prior to the drawdown have not formed. Invasion of willow (Salix sp.) and red maple (Acer rubrum) occurred along the lake shoreline during drawdown. The red maples along the present shoreline are beginning to show evidence of stress and mortality from flooding over the past four months. Some of the willows appear to be stressed as well. The loblolly pines (Pinus taeda), which were flooded in all but the shallow shoreline areas, are now dead. Future surveys are planned for the growing seasons of 1995, 1996, and 1997, along with the evaluation of satellite data for mapping the areal extent of the macrophyte beds of Par Pond

  2. Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae).

    Science.gov (United States)

    Itaya, N M; Figueiredo-Ribeiro, R C; Buckeridge, M S

    1999-04-01

    Sucrose:sucrose fructosyltransferase (SST) and fructan:fructan fructosyl-transferase (FFT) activities from crude extracts of tuberous roots of Viguiera discolor growing in a preserved area of cerrado were analyzed in 1995-1996. SST activity was characterized by the synthesis of 1-kestose from sucrose and FFT activity by the production of nystose from 1-kestose. The highest fructan-synthesizing activity was observed during early dormancy (autumn), when both (SST and FFT) activities were high. The increase in synthetic activity seemed to start during the fruiting phase in the summer, when SST activity was higher than in spring. During winter and at the beginning of sprouting, both activities declined. The in vitro synthesis of high molecular mass fructans from sucrose by enzymatic preparations from tuberous roots collected in summer showed that long incubations of up to 288 h produced consistently longer polymers which resembled those found in vivo with respect to chromatographic profiles.

  3. Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae

    Directory of Open Access Journals (Sweden)

    Itaya N.M.

    1999-01-01

    Full Text Available Sucrose:sucrose fructosyltransferase (SST and fructan:fructan fructosyl-transferase (FFT activities from crude extracts of tuberous roots of Viguiera discolor growing in a preserved area of cerrado were analyzed in 1995-1996. SST activity was characterized by the synthesis of 1-kestose from sucrose and FFT activity by the production of nystose from 1-kestose. The highest fructan-synthesizing activity was observed during early dormancy (autumn, when both (SST and FFT activities were high. The increase in synthetic activity seemed to start during the fruiting phase in the summer, when SST activity was higher than in spring. During winter and at the beginning of sprouting, both activities declined. The in vitro synthesis of high molecular mass fructans from sucrose by enzymatic preparations from tuberous roots collected in summer showed that long incubations of up to 288 h produced consistently longer polymers which resembled those found in vivo with respect to chromatographic profiles.

  4. Younger-Dryas cooling and sea-ice feedbacks were prominent features of the Pleistocene-Holocene transition in Arctic Alaska

    Science.gov (United States)

    Gaglioti, Benjamin V.; Mann, Daniel H.; Wooller, Matthew J.; Jones, Benjamin M.; Wiles, Gregory C.; Groves, Pamela; Kunz, Michael L.; Baughman, Carson A.; Reanier, Richard E.

    2017-08-01

    Declining sea-ice extent is currently amplifying climate warming in the Arctic. Instrumental records at high latitudes are too short-term to provide sufficient historical context for these trends, so paleoclimate archives are needed to better understand the functioning of the sea ice-albedo feedback. Here we use the oxygen isotope values of wood cellulose in living and sub-fossil willow shrubs (δ18Owc) (Salix spp.) that have been radiocarbon-dated (14C) to produce a multi-millennial record of climatic change on Alaska's North Slope during the Pleistocene-Holocene transition (13,500-7500 calibrated 14C years before present; 13.5-7.5 ka). We first analyzed the spatial and temporal patterns of δ18Owc in living willows growing at upland sites and found that over the last 30 years δ18Owc values in individual growth rings correlate with local summer temperature and inter-annual variations in summer sea-ice extent. Deglacial δ18Owc values from 145 samples of subfossil willows clearly record the Allerød warm period (∼13.2 ka), the Younger Dryas cold period (12.9-11.7 ka), and the Holocene Thermal Maximum (11.7-9.0 ka). The magnitudes of isotopic changes over these rapid climate oscillations were ∼4.5‰, which is about 60% of the differences in δ18Owc between those willows growing during the last glacial period and today. Modeling of isotope-precipitation relationships based on Rayleigh distillation processes suggests that during the Younger Dryas these large shifts in δ18Owc values were caused by interactions between local temperature and changes in evaporative moisture sources, the latter controlled by sea ice extent in the Arctic Ocean and Bering Sea. Based on these results and on the effects that sea-ice have on climate today, we infer that ocean-derived feedbacks amplified temperature changes and enhanced precipitation in coastal regions of Arctic Alaska during warm times in the past. Today, isotope values in willows on the North Slope of Alaska are similar

  5. Coevolution of floodplain and riparian forest dynamics on large, meandering rivers

    Science.gov (United States)

    Stella, J. C.; Riddle, J. D.; Battles, J. J.

    2012-12-01

    On large meandering rivers, riparian forests coevolve with the floodplains that support them. Floodplain characteristics such as local disturbance regime, deposition rates and sediment texture drive plant community dynamics, which in turn feed back to the abiotic processes. We investigated floodplain and riparian forest coevolution along the along the Sacramento River (California, USA), a large, mediterranean-climate river that has been extensively regulated for 70 years, but whose 160-km middle reach (Red Bluff to Colusa) retains some channel mobility and natural forest stands. Guided by maps of floodplain change over time and current vegetation cover, we conducted an extensive forest inventory and chronosequence analysis to quantify how abiotic conditions and forest structural characteristics such as tree density, basal area and biomass vary with floodplain age. We inventoried 285 fixed-area plots distributed across 19 large point bars within vegetation patches ranging in age from 4 to 107 years. Two successional trajectories were evident: (1) shifting species dominance over time within forested areas, from willow to cottonwood to walnut, boxelder and valley oak; and (2) patches of shrub willow (primarily Salix exigua) that maintained dominance throughout time. Sediment accretion was reduced in the persistent willow plots compared to the successional forest stands, suggesting an association between higher flood energy and arrested succession. Forested stands 40-60 years old were the most extensive across the chronosequence in terms of floodplain area, and supported the highest biomass, species diversity, and functional wildlife habitat. These stands were dominated by Fremont cottonwood (Populus fremontii) and reached their maxima in terms of tree size and biomass at age 50 years. The persistent willow stands reached their structural maxima earlier (32 years) and supported lower biomass. Basal area and abundance of large trees decreased in stands >90 years old

  6. Younger-Dryas cooling and sea-ice feedbacks were prominent features of the Pleistocene-Holocene transition in Arctic Alaska

    Science.gov (United States)

    Gaglioti, Benjamin V.; Mann, Daniel H.; Wooller, Matthew J.; Jones, Benjamin M.; Wiles, Gregory C.; Groves, Pamela; Kunz, Michael L.; Baughman, Carson; Reanier, Richard E.

    2017-01-01

    Declining sea-ice extent is currently amplifying climate warming in the Arctic. Instrumental records at high latitudes are too short-term to provide sufficient historical context for these trends, so paleoclimate archives are needed to better understand the functioning of the sea ice-albedo feedback. Here we use the oxygen isotope values of wood cellulose in living and sub-fossil willow shrubs (δ18Owc) (Salix spp.) that have been radiocarbon-dated (14C) to produce a multi-millennial record of climatic change on Alaska's North Slope during the Pleistocene-Holocene transition (13,500–7500 calibrated 14C years before present; 13.5–7.5 ka). We first analyzed the spatial and temporal patterns of δ18Owc in living willows growing at upland sites and found that over the last 30 years δ18Owc values in individual growth rings correlate with local summer temperature and inter-annual variations in summer sea-ice extent. Deglacial δ18Owcvalues from 145 samples of subfossil willows clearly record the Allerød warm period (∼13.2 ka), the Younger Dryas cold period (12.9–11.7 ka), and the Holocene Thermal Maximum (11.7–9.0 ka). The magnitudes of isotopic changes over these rapid climate oscillations were ∼4.5‰, which is about 60% of the differences in δ18Owc between those willows growing during the last glacial period and today. Modeling of isotope-precipitation relationships based on Rayleigh distillation processes suggests that during the Younger Dryas these large shifts in δ18Owc values were caused by interactions between local temperature and changes in evaporative moisture sources, the latter controlled by seaice extent in the Arctic Ocean and Bering Sea. Based on these results and on the effects that sea-ice have on climate today, we infer that ocean-derived feedbacks amplified temperature changes and enhanced precipitation in coastal regions of Arctic Alaska during warm times in the past. Today, isotope values in willows on the North Slope of Alaska are

  7. 21st Session of the International Poplar Commission (IPC-2000): poplar and willow culture: meeting the needs of society and the environment; 200 September 24-28; Vancouver, WA.

    Science.gov (United States)

    J.G. Isebrands; J. Richardson

    2000-01-01

    Research results and ongoing research activities on poplar and willow breeding, diseases, insects, production, and utilization are described in 220 abstracts from the International Poplar Commission meeting in Vancouver, Washington, September 24-28, 2000.

  8. Phytoremediation potential of poplar and willow species in small scale constructed wetland for boron removal.

    Science.gov (United States)

    Yıldırım, Kubilay; Kasım, Gözde Çıtır

    2018-03-01

    Boron (B) pollution is an expanding environmental problem throughout the world due to intensive mining practices and extensive usage of B in agricultural chemicals and industrial products in recent years. The purpose of this study was to investigate B removal performance of four poplar and four willow species in small scale Constructed Wetland (CW). Rooted cuttings of tested species were treated with simulated wastewater having five elevated B concentrations (0.5, 5, 10, 20 and 40 ppm). All the tested species could resist up to 20 ppm wastewater B supply and could regrow from their roots in the soil having maximum 15 mg/kg B content. The result of the study indicated that 65% ± 5.3 of B was removed from the wastewater in 5 ppm B treatment while the same efficiency decreased to 45% ± 4.6 at 40 ppm B supply. The average effect of sediment on B removal was found to be approximately 20% for all B treatments while the remaining part of the loaded B was removed from the CW within effluent (35-54%). Therefore, actual effects of plant species on B removal was ranged from 45% to 25% between 5 and 40 ppm B treatments. Mass B removal within plant body (phytextraction) comprised the 13-10% of total loaded B in CW while the remaining part of the loaded B (31-15%) was stabilized into the sediment with the effects of poplar and willow roots. These results presented clear understanding of effective B purification mechanisms in CWs. Boron phytextraction capacity of a plant species was less effective than its phytstabilization efficiency which increase filtering capacity of the sediment and stabilization of more B around the rhizosphere. In terms of their B removal ability, P.nigra and S.anatolica had the highest B removal capacities with phytextraction (20-11%) while S.alba, P.alba and S.babylonica had more phytstabilizaiton performance (40-15%) in CW. Disposal of B loaded plant material create another environmental costs for CW applications. Therefore, B loaded

  9. Building energetic material from novel salix leaf-like CuO and nano-Al through electrophoretic deposition

    Energy Technology Data Exchange (ETDEWEB)

    Yin, Yan Jun; Li, Xueming [College of Chemistry and Chemical Engineering, Chongqing University, Chongqing (China)

    2016-11-15

    In this study, an energetic material was prepared by depositing nano-Al on CuO arrays via electrophoretic deposition (EPD), which offers a feasible route for nano-Al integration. The morphology and structure of the CuO arrays and Al/CuO composites were characterized by scanning electron microscopy and X-ray diffraction. The CuO arrays were homogenously salix leaf-like structure with a width of ⁓150 to 200 nm. The energy density of Al/CuO composites was approximate to 1454.5 J/g by integrating the differential scanning calorimetry (DSC) plot and the combustion performance was recorded by a high-speed camera. Moreover, the combustion flames were violent and the whole reaction process only lasted 72.2 ms, indicating that the energy of the Al/CuO nanothermite can be released effectively.

  10. High-Arctic Plant-Herbivore Interactions under Climate Influence

    DEFF Research Database (Denmark)

    Berg, Thomas B.; Schmidt, Niels M.; Høye, Toke Thomas

    This chapter focuses on a 10-year data series from Zackenberg on the trophic interactions between two characteristic arctic plant species, arctic willow Salix arctica and mountain avens Dryas octopetala, and three herbivore species covering the very scale of size present at Zackenberg, namely......, the moth Sympistis zetterstedtii, the collared lemming Dicrostonyx groenlandicus and the musk ox Ovibos moschatus. Data from Zackenberg show that timing of snowmelt, the length of the growing season and summer temperature are the basic variables that determine the phenology of flowering and primary...... production upon which the herbivores depend, and snow may be the most important climatic factor affecting the different trophic levels and the interactions between them. Hence, the spatio-temporal distribution of snow, as well as thawing events during winter, may have considerable effects on the herbivores...

  11. Short-rotation Willow Biomass Plantations Irrigated and Fertilised with Wastewaters. Results from a 4-year multidisciplinary field project in Sweden, France, Northern Ireland and Greece

    Energy Technology Data Exchange (ETDEWEB)

    Larsson, Stig [Svaloef Weibull AB, Svaloef (Sweden); Cuingnet, Christian; Clause, Pierre [Association pour le Developpement des Culture Energetiques, Lille (France); Jakobsson, Ingvar [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden); Dawson, Malcolm [Queens Univ., Northern Ireland (United Kingdom); Backlund, Arne [A and B Backlund ApS, Charlottenlund (Denmark); Mavrogianopoulus, George [Agricultural Univ. of Athens (Greece)

    2003-01-01

    This report summarises results and experiences gathered from field trials with recycling of pre-treated wastewater, diverted human urine mixed with water, and municipal sludge, within plantations of willow species specifically selected for biomass production. Experimental sites were established in Sweden (Roma), France (Orchies), Northern Ireland (Culmore) and Greece (Larissa). The project was carried out during a 4-year period with financial support from the EU FAIR Programme. The experimental sites were supplied with primary effluent from municipal treatment plants (Culmore and Larissa), stored industrial effluent from a chicory processing plant (Orchies), biologically treated and stored municipal wastewater (Roma) and human urine mixture from diverting low-flush toilets mixed with water (Roma). Application rates of the wastewaters or the urine mixture were equivalent to the calculated evapotranspiration rate at each site. Wastewaters were also applied up to three times this value to evaluate any possible negative effects. Estimations and evaluations were carried out mainly concerning: biomass growth, potential biological attacks of the plantations, plant water requirements, fertilisation effects of the wastewater, plant uptake of nutrients and heavy metals from applied wastewater, possible soil or groundwater impact, sanitary aspects, and potentials for removal in the soil-plant filter of nutrients and biodegradable organic material from applied wastewater. The results clearly indicated that biomass production in young willow plantations could be enhanced substantially after recycling of wastewater resources. The impact on soil and groundwater of nutrients (nitrogen and phosphorus) and heavy metals (copper, zinc, lead and cadmium) was limited, even when the application of water and nutrients exceeded the plant requirements. Also, the soil-plant system seemed to function as a natural treatment filter for pre-treated (primary settled) wastewater, with a treatment

  12. IAA oxidase activity in relation to adventitious root formation on stem cuttings of some forest tree species. [Salix tetrasperma, Populus Robusta, Hibiscus rosa-sinensis, Eucalyptus citriodora

    Energy Technology Data Exchange (ETDEWEB)

    Bansal, M.P.; Nanda, K.K.

    1981-01-01

    In rooting tests with stem cuttings, IAA oxidase activity was found to be very high in Salix tetrasperma and Populus 'Robusta' both of which rooted profusely, less in Hibiscus rosa-sinensis which rooted but weakly and insignificant in Eucalyptus citriodora, which did not root at all. Proteins extracted from the stem cuttings of E. citriodora inhibited IAA oxidase activity, and also root formation on hypocotyl cuttings of Phaseolus mungo.

  13. A comparative, randomized, controlled study on clinical efficacy and dental staining reduction of a mouthwash containing Chlorhexidine 0.20% and Anti Discoloration System (ADS).

    Science.gov (United States)

    Marrelli, Massimo; Amantea, Massimiliano; Tatullo, Marco

    2015-01-01

    A good control of bacterial plaque is an essential factor for the success of periodontal therapy, therefore it is the main objective that the clinician together with the patient must get to have a healthy periodontium. The plaque control with mouthwashes is the most important home therapy as it helps to reduce the formation of plaque between the mechanical removal with a toothbrush. Authors analyzed the clinical data from a trial carried out with 3 different mouthwashes containing 0.2% Chlorhexidine (CHX). In addition, the ADS (Anti Discoloration System - Curaden Healthcare) was tested in comparison with the other mouthwashes without this system. We tested antiplaque activity showed by 3 of the most commercialized mouthwashes, moreover, we tested the ability in reducing the dental staining related to the oral assumption of Chlorhexidine. Our results demonstrated the clinical efficacy of the 3 mouthwashes with CHX. Particularly performing was the anti discoloration system (Curaden Healthcare), with a clinical detection of dental stainings significantly less than the others tested. This study demonstrated the clinical efficacy of ADS system in the reduction of tooth staining, without a loss of antiplaque activity with respect to the competing mouthwashes containing CHX.

  14. Organic Carbon Accumulation in Topsoil Following Afforestation with Willow: Emphasis on Leaf Litter Decomposition and Soil Organic Matter Quality

    Directory of Open Access Journals (Sweden)

    Benoit Lafleur

    2015-03-01

    Full Text Available Short-rotation intensive cultures (SRICs of willows can potentially sequester carbon (C in soil. However, there is limited information regarding the factors governing soil organic C (Corg accumulation following afforestation. The objectives of this study were to: (i determine whether willow leads to Corg accumulation in the topsoil (0–10 cm two to six years after establishment in five SRICs located along a large climatic/productivity gradient in southern Quebec, and (ii assess the influence of leaf litter decomposition and soil organic matter (OM quality on Corg accumulation in the topsoil. Topsoil Corg concentrations and pools under SRICs were, on average, 25% greater than reference fields, and alkyls concentrations were higher under SRICs. On an annualized basis, Corg accumulation rates in the topsoil varied between 0.4 and 4.5 Mg ha−1 yr−1. Estimated annual litterfall C fluxes were in the same order of magnitude, suggesting that SRICs can accumulate Corg in the topsoil during early years due to high growth rates. Leaf litter decomposition was also related to Corg accumulation rates in the topsoil. It was positively correlated to growing season length, degree-days, and growing season average air and topsoil temperature (r > 0.70, and negatively correlated to topsoil volumetric water content (r = −0.55. Leaf litter decomposition likely occurred more quickly than that of plants in reference fields, and as it progressed, OM became more decay resistant, more stable and accumulated as Corg in the topsoil.

  15. Mechanism of degradation and discoloration reaction of L-ascorbic acid

    International Nuclear Information System (INIS)

    Kurata, Tadao

    1976-01-01

    The mechanisms of decomposition and coloration reaction of L-ascorbic acid are reviewed. At the initial stage of the decomposition, it can be classified roughly into oxidative and non-oxidative processes of decomposition. ASA forms furfural by being heated and decomposed in strong acid. The mechanism of the production of furfural at varying pH in acidic region was discussed. Furfural was produced through the enol form of 3-deoxy-L-pentosulose(3DP). 3DP seemed to be produced by two different routes: the one route consists of successive reactions from ASA through lactone ring-opening, dehydration, decarboxylation, to 3DP, and the other consists of reactions from the 3-keto form of ASA, through lactone ring-opening, decarboxylation, and dehydration, to the enol form of 3DP. ASA is easily reduced and decomposed through dehydro-ASA(DHA) by the presence of an oxidizing agent. The decomposition of DHA is discussed in cases of the systems of DHA alone, DHA and α-aminoacid, and DHA and amine. DHA was decomposed by the same reaction scheme as the decomposition of ASA and yielded 2-furoic acid. In the presence of an amino acid, DHA was decomposed by the Strecker decomposition, and yielded a red compound and a radical. In the presence of an amine, the discoloration reaction seemed to take place through radical reaction mechanism. The coloration reaction of ASA occurs in an acidic medium, and is accelerated by the oxidative process of decomposition. (Nishino, S.)

  16. Incorporating climate change and exotic species into forecasts of riparian forest distribution.

    Directory of Open Access Journals (Sweden)

    Dana H Ikeda

    Full Text Available We examined the impact climate change (CC will have on the availability of climatically suitable habitat for three native and one exotic riparian species. Due to its increasing prevalence in arid regions throughout the western US, we predicted that an exotic species, Tamarix, would have the greatest increase in suitable habitat relative to native counterparts under CC. We used an ecological niche model to predict range shifts of Populus fremontii, Salix gooddingii, Salix exigua and Tamarix, from present day to 2080s, under five general circulation models and one climate change scenario (A1B. Four major findings emerged. 1 Contrary to our original hypothesis, P. fremontii is projected to have the greatest increase in suitable habitat under CC, followed closely by Tamarix. 2 Of the native species, S. gooddingii and S. exigua showed the greatest loss in predicted suitable habitat due to CC. 3 Nearly 80 percent of future P. fremontii and Salix habitat is predicted to be affected by either CC or Tamarix by the 2080s. 4 By the 2080s, 20 percent of S. gooddingii habitat is projected to be affected by both Tamarix and CC concurrently, followed by S. exigua (19 percent and P. fremontii (13 percent. In summary, while climate change alone will negatively impact both native willow species, Tamarix is likely to affect a larger portion of all three native species' distributions. We discuss these and other results in the context of prioritizing restoration and conservation efforts to optimize future productivity and biodiversity. As we are accounting for only direct effects of CC and Tamarix on native habitat, we present a possible hierarchy of effects- from the direct to the indirect- and discuss the potential for the indirect to outweigh the direct effects. Our results highlight the need to account for simultaneous challenges in the face of CC.

  17. Tamarisk control, water salvage, and wildlife habitat restoration along rivers in the western United States

    Science.gov (United States)

    Shafroth, Patrick B.

    2006-01-01

    In the latter part of the 19th century, species of the nonnative shrub tamarisk (also called saltcedar; for example, Tamarix ramosissima, T. chinensis) were introduced to the United States for use as ornamental plants for erosion control. By 1877, some naturalized populations had become established, and by the 1960s, tamarisk was present along most rivers in the semi-arid and arid parts of the West and was quite abundant along downstream ranches of the major southwest rivers such as the Colorado, Rio Grande, Gila, and Pecos. The principal period of tamarisk invasion coincided with changing physical conditions along western rivers associated with the construction and operation of dams. In many cases, these altered physical conditions appear to have been more favorable for tamarisk than native riparian competitors like cottonwoods and willows (Populus and Salix; Glenn and Nagler, 2005).

  18. Conductivity gradients as inferred by electromagnetic-induction meter (EM38) readings within a salt-affected wetland in Saskatchewan, Canada

    Science.gov (United States)

    Mirck, Jaconette; Schroeder, William

    2018-01-01

    The change from deep-rooted grass and shrub vegetation to annual-cropping dryland farming has contributed to serious soil salinization challenges on the semi-arid North American Great Plains. In some cases, cultivation of the Great Plains has increased the availability of water, causing dominant sulfate salts to travel from the uphill areas to depressions where it will surface when water evaporates at the soil surface. A potential solution could include the replanting of the native deep-rooted vegetation, which requires knowledge of the spatial distribution of soil salinity. This study tested the soil factors influencing electromagnetic-induction meter (EM38) readings of soil salinity distribution around wetlands. The objectives were to: (1) predict growth and survival of Salix dasyclados Wimm. (cv. `India') along a salinity gradient in a small wetland, and (2) investigate whether newly established willows affected water-table fluctuations, which would indicate their phreatophytic nature or their ability to obtain their water supply from the zone of saturation. Results indicated significantly lower salinity values for sampling points with EM38 readings above 175 and 250 mS m-1 for height and survival, respectively. In addition, diurnal fluxes of the water table in areas of good willow growth and lower salinity indicated that cultivar `India' was phreatophytic in these areas and therefore has great potential for being used to combat saline seeps.

  19. Food habits of the southwestern willow flycatcher during the nesting season

    Science.gov (United States)

    Drost, Charles A.; Paxton, Eben H.; Sogge, Mark K.; Whitfield, Mary J.

    2003-01-01

    The food habits and prey base of the endangered Southwestern Willow Flycatcher (Empidonax traillii extimus) are not well known. We analyzed prey remains in 59 fecal samples from an intensively-studied population of this flycatcher at the Kern River Preserve in southern California. These samples were collected during the nesting season in 1996 and 1997 from adults caught in mist nets, and from nestlings temporarily removed from the nest for banding. A total of 379 prey individuals were identified in the samples. Dominant prey taxa, both in total numbers and in percent occurrence, were true bugs (Hemiptera), flies (Diptera), and beetles (Coleoptera). Leafhoppers (Homoptera: Cicadellidae), spiders (Araneae), bees and wasps (Hymenoptera), and dragonflies and damselflies (Odonata) were also common items. Diet composition was significantly different between years, due to a large difference in the numbers of spiders between 1996 and 1997. There was also a significant difference between the diet of young and adults, with the diet of young birds having significantly higher numbers of odonates and beetles. There was a trend toward diet differences between males and females, but this was not significant at the P = 0.05 level.

  20. Response of Organ Structure and Physiology to Autotetraploidization in Early Development of Energy Willow Salix viminalis

    Czech Academy of Sciences Publication Activity Database

    Dudits, D.; Torok, K.; Cseri, A.; Paul, K.; Nagy, A.V.; Nagy, B.; Sass, L.; Ferenc, G.; Vaňková, Radomíra; Dobrev, Petre; Vass, I.; Ayaydin, F.

    2016-01-01

    Roč. 170, č. 3 (2016), s. 1504-1523 ISSN 0032-0889 Institutional support: RVO:61389030 Keywords : IMAGE-ANALYSIS * WATER -STRESS * PHOTOSYNTHETIC RESPONSES Subject RIV: EF - Botanics Impact factor: 6.456, year: 2016