Research of x-ray automatic image mosaic method

Science.gov (United States)

Liu, Bin; Chen, Shunan; Guo, Lianpeng; Xu, Wanpeng

2013-10-01

Image mosaic has widely applications value in the fields of medical image analysis, and it is a technology that carries on the spatial matching to a series of image which are overlapped with each other, and finally builds a seamless and high quality image which has high resolution and big eyeshot. In this paper, the method of grayscale cutting pseudo-color enhancement was firstly used to complete the mapping transformation from gray to the pseudo-color, and to extract SIFT features from the images. And then by making use of a similar measure of NCC (normalized cross correlation - Normalized cross-correlation), the method of RANSAC (Random Sample Consensus) was used to exclude the pseudofeature points right in order to complete the exact match of feature points. Finally, seamless mosaic and color fusion were completed by using wavelet multi-decomposition. The experiment shows that the method we used can effectively improve the precision and automation of the medical image mosaic, and provide an effective technical approach for automatic medical image mosaic.

SHOK—The First Russian Wide-Field Optical Camera in Space

Science.gov (United States)

Lipunov, V. M.; Gorbovskoy, E. S.; Kornilov, V. G.; Panasyuk, M. I.; Amelushkin, A. M.; Petrov, V. L.; Yashin, I. V.; Svertilov, S. I.; Vedenkin, N. N.

2018-02-01

Onboard the spacecraft Lomonosov is established two fast, fixed, very wide-field cameras SHOK. The main goal of this experiment is the observation of GRB optical emission before, synchronously, and after the gamma-ray emission. The field of view of each of the cameras is placed in the gamma-ray burst detection area of other devices located onboard the "Lomonosov" spacecraft. SHOK provides measurements of optical emissions with a magnitude limit of ˜ 9-10m on a single frame with an exposure of 0.2 seconds. The device is designed for continuous sky monitoring at optical wavelengths in the very wide field of view (1000 square degrees each camera), detection and localization of fast time-varying (transient) optical sources on the celestial sphere, including provisional and synchronous time recording of optical emissions from the gamma-ray burst error boxes, detected by the BDRG device and implemented by a control signal (alert trigger) from the BDRG. The Lomonosov spacecraft has two identical devices, SHOK1 and SHOK2. The core of each SHOK device is a fast-speed 11-Megapixel CCD. Each of the SHOK devices represents a monoblock, consisting of a node observations of optical emission, the electronics node, elements of the mechanical construction, and the body.

On-Line High Dose-Rate Gamma Ray Irradiation Test of the CCD/CMOS Cameras

Energy Technology Data Exchange (ETDEWEB)

Cho, Jai Wan; Jeong, Kyung Min [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

2012-05-15

In this paper, test results of gamma ray irradiation to CCD/CMOS cameras are described. From the CAMS (containment atmospheric monitoring system) data of Fukushima Dai-ichi nuclear power plant station, we found out that the gamma ray dose-rate when the hydrogen explosion occurred in nuclear reactors 1{approx}3 is about 160 Gy/h. If assumed that the emergency response robot for the management of severe accident of the nuclear power plant has been sent into the reactor area to grasp the inside situation of reactor building and to take precautionary measures against releasing radioactive materials, the CCD/CMOS cameras, which are loaded with the robot, serve as eye of the emergency response robot. In the case of the Japanese Quince robot system, which was sent to carry out investigating the unit 2 reactor building refueling floor situation, 7 CCD/CMOS cameras are used. 2 CCD cameras of Quince robot are used for the forward and backward monitoring of the surroundings during navigation. And 2 CCD (or CMOS) cameras are used for monitoring the status of front-end and back-end motion mechanics such as flippers and crawlers. A CCD camera with wide field of view optics is used for monitoring the status of the communication (VDSL) cable reel. And another 2 CCD cameras are assigned for reading the indication value of the radiation dosimeter and the instrument. In the preceding assumptions, a major problem which arises when dealing with CCD/CMOS cameras in the severe accident situations of the nuclear power plant is the presence of high dose-rate gamma irradiation fields. In the case of the DBA (design basis accident) situations of the nuclear power plant, in order to use a CCD/CMOS camera as an ad-hoc monitoring unit in the vicinity of high radioactivity structures and components of the nuclear reactor area, a robust survivability of this camera in such intense gamma-radiation fields therefore should be verified. The CCD/CMOS cameras of various types were gamma irradiated at a

Design of area array CCD image acquisition and display system based on FPGA

Science.gov (United States)

Li, Lei; Zhang, Ning; Li, Tianting; Pan, Yue; Dai, Yuming

2014-09-01

With the development of science and technology, CCD(Charge-coupled Device) has been widely applied in various fields and plays an important role in the modern sensing system, therefore researching a real-time image acquisition and display plan based on CCD device has great significance. This paper introduces an image data acquisition and display system of area array CCD based on FPGA. Several key technical challenges and problems of the system have also been analyzed and followed solutions put forward .The FPGA works as the core processing unit in the system that controls the integral time sequence .The ICX285AL area array CCD image sensor produced by SONY Corporation has been used in the system. The FPGA works to complete the driver of the area array CCD, then analog front end (AFE) processes the signal of the CCD image, including amplification, filtering, noise elimination, CDS correlation double sampling, etc. AD9945 produced by ADI Corporation to convert analog signal to digital signal. Developed Camera Link high-speed data transmission circuit, and completed the PC-end software design of the image acquisition, and realized the real-time display of images. The result through practical testing indicates that the system in the image acquisition and control is stable and reliable, and the indicators meet the actual project requirements.

Phase shifting white light interferometry using colour CCD for optical metrology and bio-imaging applications

Science.gov (United States)

Upputuri, Paul Kumar; Pramanik, Manojit

2018-02-01

Phase shifting white light interferometry (PSWLI) has been widely used for optical metrology applications because of their precision, reliability, and versatility. White light interferometry using monochrome CCD makes the measurement process slow for metrology applications. WLI integrated with Red-Green-Blue (RGB) CCD camera is finding imaging applications in the fields optical metrology and bio-imaging. Wavelength dependent refractive index profiles of biological samples were computed from colour white light interferograms. In recent years, whole-filed refractive index profiles of red blood cells (RBCs), onion skin, fish cornea, etc. were measured from RGB interferograms. In this paper, we discuss the bio-imaging applications of colour CCD based white light interferometry. The approach makes the measurement faster, easier, cost-effective, and even dynamic by using single fringe analysis methods, for industrial applications.

Web Map Services (WMS) Global Mosaic

Science.gov (United States)

Percivall, George; Plesea, Lucian

2003-01-01

The WMS Global Mosaic provides access to imagery of the global landmass using an open standard for web mapping. The seamless image is a mosaic of Landsat 7 scenes; geographically-accurate with 30 and 15 meter resolutions. By using the OpenGIS Web Map Service (WMS) interface, any organization can use the global mosaic as a layer in their geospatial applications. Based on a trade study, an implementation approach was chosen that extends a previously developed WMS hosting a Landsat 5 CONUS mosaic developed by JPL. The WMS Global Mosaic supports the NASA Geospatial Interoperability Office goal of providing an integrated digital representation of the Earth, widely accessible for humanity's critical decisions.

Identification of virus isolates inducing mosaic of sugarcane in ...

African Journals Online (AJOL)

Sugarcane mosaic disease caused by sugarcane mosaic virus (SCMV), Johnsongrass mosaic virus (JGMV), maize dwarf mosaic virus (MDMV) and sorghum mosaic Virus (SrMV) is an economically important viral disease of sugarcane worldwide. Field survey was conducted to assess the presence of the viruses involve in ...

BVRI CCD photometry of Omega Centauri

International Nuclear Information System (INIS)

Alcaino, G.; Liller, W.

1987-01-01

Color-magnitude diagrams (CMDs) of V vs B-V, V vs V-I, and V vs B-I have been constructed based on 179 BVRI CCD frames of two adjoining 4x2.5-arcmin fields in Omega Cen (NGC 5139) obtained with the 1.54-m Danish La Silla telescope. The spread in the main sequences noted in the three CMDs indicates that the wide range in chemical composition among the evolved stars in this cluster persists as well in the unevolved stars. This result suggests that the abundance variations are primordial. A difference in magnitude between the turnoff and the horizontal branch of 3.8 + or - 0.15 is found which is greater than a previous value. 38 references

Velvet bean severe mosaic virus: a distinct begomovirus species causing severe mosaic in Mucuna pruriens (L.) DC.

Science.gov (United States)

Zaim, Mohammad; Kumar, Yogesh; Hallan, Vipin; Zaidi, A A

2011-08-01

Velvet bean [Mucuna pruriens (L.) DC] is one of the most important medicinal plants. It is used to treat many ailments, but is widely used for the treatment especially for Parkinson's disease because of the presence of 3,4-dihydroxyphenylalanine (L-dopa) in it. It was noticed in last 5 years that the plants in the field showed severe mosaic, downward curling of the leaves, stunting, etc. This is consistently observed over the years in India. The disease was transmitted by whiteflies and by grafting and the causal agent was found to be a bipartite begomovirus. The whole genome was amplified by rolling circle amplification (RCA) using ϕ-29 DNA polymerase and characterized. DNA-A and DNA-B shared a 124-nucleotide (nt) long highly conserved (98%) common region (CR). Comparisons with other begomovirus showed that DNA-A sequence has highest identity (76%) with an isolate of Mungbean yellow mosaic India virus (MYMIV; AY937195) reported from India. This data suggested that the present isolate is a new species of genus Begomovirus for which the name "Velvet bean severe mosaic virus" (VbSMV) is proposed. DNA-B has a maximum sequence identity of 49% with an isolate of Horsegram yellow mosaic virus (HgYMV; AM932426) reported from India. Infectious clones consisting of a 1.7 mer partial tandem repeat of DNA-A and a dimer of DNB-B were constructed and agro-inoculated to Macuna pruriens (L.) DC plants, which showed field observed symptoms 24 days post-infiltration (dpi). In phylogenetic analysis, DNA-A and DNA-B of the present isolate grouped with DNA-A of different begomoviruses reported from fabaceous crops. The study presents first ever molecular evidence of any disease in velvet bean and whole genome analysis of the causative virus which is a distinct bipartite species of Begomovirus.

UVUDF: Ultraviolet Imaging of the Hubble Ultra Deep Field with Wide-Field Camera 3

Science.gov (United States)

Teplitz, Harry I.; Rafelski, Marc; Kurczynski, Peter; Bond, Nicholas A.; Grogin, Norman; Koekemoer, Anton M.; Atek, Hakim; Brown, Thomas M.; Coe, Dan; Colbert, James W.; Ferguson, Henry C.; Finkelstein, Steven L.; Gardner, Jonathan P.; Gawiser, Eric; Giavalisco, Mauro; Gronwall, Caryl; Hanish, Daniel J.; Lee, Kyoung-Soo; de Mello, Duilia F.; Ravindranath, Swara; Ryan, Russell E.; Siana, Brian D.; Scarlata, Claudia; Soto, Emmaris; Voyer, Elysse N.; Wolfe, Arthur M.

2013-12-01

We present an overview of a 90 orbit Hubble Space Telescope treasury program to obtain near-ultraviolet imaging of the Hubble Ultra Deep Field using the Wide Field Camera 3 UVIS detector with the F225W, F275W, and F336W filters. This survey is designed to: (1) investigate the episode of peak star formation activity in galaxies at 1 dropouts at redshifts 1.7, 2.1, and 2.7 is largely consistent with the number predicted by published luminosity functions. We also confirm that the image mosaics have sufficient sensitivity and resolution to support the analysis of the evolution of star-forming clumps, reaching 28-29th magnitude depth at 5σ in a 0.''2 radius aperture depending on filter and observing epoch. Based on observations made with the NASA/ESA Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-26555. These observations are #12534.

New design for the UCO/Lick Observatory CCD guide camera

Science.gov (United States)

Wei, Mingzhi; Stover, Richard J.

1996-03-01

A new CCD based field acquisition and telescope guiding camera is being designed and built at UCO/Lick Observatory. Our goal is a camera which is fully computer controllable, compact in size, versatile enough to provide a wide variety of image acquisition modes, and able to operate with a wide variety of CCD detectors. The camera will improve our remote-observing capabilities since it will be easy to control the camera and obtain images over the Observatory computer network. To achieve the desired level of operating flexibility, the design incorporates state-of-the-art technologies such as high density, high speed programmable logic devices and non-volatile static memory. Various types of CCDs can be used in this system without major modification of the hardware or software. Though fully computer controllable, the camera can be operated as a stand-alone unit with most operating parameters set locally. A stand-alone display subsystem is also available. A thermoelectric device is used to cool the CCD to about -45c. Integration times can be varied over a range of 0.1 to 1000 seconds. High speed pixel skipping in both horizontal and vertical directions allows us to quickly access a selected subarea of the detector. Three different read out speeds allow the astronomer to select between high-speed/high-noise and low-speed/low-noise operation. On- chip pixel binning and MPP operation are also selectable options. This system can provide automatic sky level measurement and subtraction to accommodate dynamically changing background levels.

The development of large-aperture test system of infrared camera and visible CCD camera

Science.gov (United States)

Li, Yingwen; Geng, Anbing; Wang, Bo; Wang, Haitao; Wu, Yanying

2015-10-01

Infrared camera and CCD camera dual-band imaging system is used in many equipment and application widely. If it is tested using the traditional infrared camera test system and visible CCD test system, 2 times of installation and alignment are needed in the test procedure. The large-aperture test system of infrared camera and visible CCD camera uses the common large-aperture reflection collimator, target wheel, frame-grabber, computer which reduces the cost and the time of installation and alignment. Multiple-frame averaging algorithm is used to reduce the influence of random noise. Athermal optical design is adopted to reduce the change of focal length location change of collimator when the environmental temperature is changing, and the image quality of the collimator of large field of view and test accuracy are also improved. Its performance is the same as that of the exotic congener and is much cheaper. It will have a good market.

Application of SFM and laser scanning technology to the description of mosaics piece by piece

Directory of Open Access Journals (Sweden)

O. Ajioka

2014-06-01

Full Text Available Mosaic floors of surviving buildings in Ostia have been mainly recorded in photographs. From 2008, Japanese research group carries out a project of 3d measuring of the whole structure of ancient Roman city Ostia using laser scanners, including its landscape, city blocks, streets, buildings, wall paintings and mosaics. The laser scanner allows for a more detailed analysis and a greater potential for recording mosaics. We can record the data of mosaics, which are described piece by piece. However it is hard to acquire enough high dense point cloud and the internal camera of the laser scanner produce low quality images. We introduce a possible technology of 3D recording of mosaics with high-quality colour information; SFM. The use of this technique permits us to create 3D models from images provided from a CCD camera without heavy and large laser scanners. We applied SFM system to different three types of the mosaics laid down on the floors of "the House of the Dioscuroi", "the Insula of the Muse" and "the House of Jove and Ganymede", and created high resolution orthographic images. Then we examined to compare these orthographic images with that are created from the point cloud data. As a result, we confirmed that SFM system has sufficient practical utility for the mosaic research. And we present how much of density of point cloud or ground resolution are required for the documentation of mosaics accurately.

Wide-Field Imaging Telescope-0 (WIT0) with automatic observing system

Science.gov (United States)

Ji, Tae-Geun; Byeon, Seoyeon; Lee, Hye-In; Park, Woojin; Lee, Sang-Yun; Hwang, Sungyong; Choi, Changsu; Gibson, Coyne Andrew; Kuehne, John W.; Prochaska, Travis; Marshall, Jennifer L.; Im, Myungshin; Pak, Soojong

2018-01-01

We introduce Wide-Field Imaging Telescope-0 (WIT0), with an automatic observing system. It is developed for monitoring the variabilities of many sources at a time, e.g. young stellar objects and active galactic nuclei. It can also find the locations of transient sources such as a supernova or gamma-ray bursts. In 2017 February, we installed the wide-field 10-inch telescope (Takahashi CCA-250) as a piggyback system on the 30-inch telescope at the McDonald Observatory in Texas, US. The 10-inch telescope has a 2.35 × 2.35 deg field-of-view with a 4k × 4k CCD Camera (FLI ML16803). To improve the observational efficiency of the system, we developed a new automatic observing software, KAOS30 (KHU Automatic Observing Software for McDonald 30-inch telescope), which was developed by Visual C++ on the basis of a windows operating system. The software consists of four control packages: the Telescope Control Package (TCP), the Data Acquisition Package (DAP), the Auto Focus Package (AFP), and the Script Mode Package (SMP). Since it also supports the instruments that are using the ASCOM driver, the additional hardware installations become quite simplified. We commissioned KAOS30 in 2017 August and are in the process of testing. Based on the WIT0 experiences, we will extend KAOS30 to control multiple telescopes in future projects.

New Subarray Readout Patterns for the ACS Wide Field Channel

Science.gov (United States)

Golimowski, D.; Anderson, J.; Arslanian, S.; Chiaberge, M.; Grogin, N.; Lim, Pey Lian; Lupie, O.; McMaster, M.; Reinhart, M.; Schiffer, F.; Serrano, B.; Van Marshall, M.; Welty, A.

2017-04-01

At the start of Cycle 24, the original CCD-readout timing patterns used to generate ACS Wide Field Channel (WFC) subarray images were replaced with new patterns adapted from the four-quadrant readout pattern used to generate full-frame WFC images. The primary motivation for this replacement was a substantial reduction of observatory and staff resources needed to support WFC subarray bias calibration, which became a new and challenging obligation after the installation of the ACS CCD Electronics Box Replacement during Servicing Mission 4. The new readout patterns also improve the overall efficiency of observing with WFC subarrays and enable the processing of subarray images through stages of the ACS data calibration pipeline (calacs) that were previously restricted to full-frame WFC images. The new readout patterns replace the original 512×512, 1024×1024, and 2048×2046-pixel subarrays with subarrays having 2048 columns and 512, 1024, and 2048 rows, respectively. Whereas the original square subarrays were limited to certain WFC quadrants, the new rectangular subarrays are available in all four quadrants. The underlying bias structure of the new subarrays now conforms with those of the corresponding regions of the full-frame image, which allows raw frames in all image formats to be calibrated using one contemporaneous full-frame "superbias" reference image. The original subarrays remain available for scientific use, but calibration of these image formats is no longer supported by STScI.

Electroless synthesis of 3 nm wide alloy nanowires inside Tobacco mosaic virus

International Nuclear Information System (INIS)

Balci, Sinan; Kern, Klaus; Bittner, Alexander M; Hahn, Kersten; Kopold, Peter; Kadri, Anan; Wege, Christina

2012-01-01

We show that 3 nm wide cobalt–iron alloy nanowires can be synthesized by simple wet chemical electroless deposition inside tubular Tobacco mosaic virus particles. The method is based on adsorption of Pd(II) ions, formation of a Pd catalyst, and autocatalytic deposition of the alloy from dissolved metal salts, reduced by a borane compound. Extensive energy-filtering TEM investigations at the nanoscale revealed that the synthesized wires are alloys of Co, Fe, and Ni. We confirmed by high-resolution TEM that our alloy nanowires are at least partially crystalline, which is compatible with typical Co-rich alloys. Ni traces bestow higher stability, presumably against corrosion, as also known from bulk CoFe. Alloy nanowires, as small as the ones presented here, might be used for a variety of applications including high density data storage, imaging, sensing, and even drug delivery. (paper)

Speckle correlation resolution enhancement of wide-field fluorescence imaging (Conference Presentation)

Science.gov (United States)

Yilmaz, Hasan

2016-03-01

Structured illumination enables high-resolution fluorescence imaging of nanostructures [1]. We demonstrate a new high-resolution fluorescence imaging method that uses a scattering layer with a high-index substrate as a solid immersion lens [2]. Random scattering of coherent light enables a speckle pattern with a very fine structure that illuminates the fluorescent nanospheres on the back surface of the high-index substrate. The speckle pattern is raster-scanned over the fluorescent nanospheres using a speckle correlation effect known as the optical memory effect. A series of standard-resolution fluorescence images per each speckle pattern displacement are recorded by an electron-multiplying CCD camera using a commercial microscope objective. We have developed a new phase-retrieval algorithm to reconstruct a high-resolution, wide-field image from several standard-resolution wide-field images. We have introduced phase information of Fourier components of standard-resolution images as a new constraint in our algorithm which discards ambiguities therefore ensures convergence to a unique solution. We demonstrate two-dimensional fluorescence images of a collection of nanospheres with a deconvolved Abbe resolution of 116 nm and a field of view of 10 µm × 10 µm. Our method is robust against optical aberrations and stage drifts, therefore excellent for imaging nanostructures under ambient conditions. [1] M. G. L. Gustafsson, J. Microsc. 198, 82-87 (2000). [2] H. Yilmaz, E. G. van Putten, J. Bertolotti, A. Lagendijk, W. L. Vos, and A. P. Mosk, Optica 2, 424-429 (2015).

Multichannel Image Mosaicing of Stem Cells

OpenAIRE

Alessandro Bevilacqua; Alessandro Gherardi; Filippo Piccinini

2010-01-01

Image mosaicing techniques are usually employed to offer researchers a wider field of view of microscopic image of biological samples. a mosaic is commonly achieved using automated microscopes and often with one “color" channel, whether it refers to natural or fluorescent analysis. In this work we present a method to achieve three subsequent mosaics of the same part of a stem cell culture analyzed in phase contrast and in fluorescence, with a common non-automated inverted microscope. The mosa...

Evolutionary relationship of alfalfa mosaic virus with cucumber mosaic virus and brome mosaic virus

OpenAIRE

Savithri, HS; Murthy, MRN

1983-01-01

The amino acid sequences of the non-structural protein (molecular weight 35,000; 3a protein) from three plant viruses - cucumber mosaic, brome mosaic and alfalfa mosaic have been systematically compared using the partial genomic sequences for these three viruses already available. The 3a protein of cucumber mosaic virus has an amino acid sequence homology of 33.7% with the corresponding protein of brome mosaic virus. A similar protein from alfalfa mosaic virus has a homology of 18.2% and 14.2...

CCD high-speed videography system with new concepts and techniques

Science.gov (United States)

Zheng, Zengrong; Zhao, Wenyi; Wu, Zhiqiang

1997-05-01

A novel CCD high speed videography system with brand-new concepts and techniques is developed by Zhejiang University recently. The system can send a series of short flash pulses to the moving object. All of the parameters, such as flash numbers, flash durations, flash intervals, flash intensities and flash colors, can be controlled according to needs by the computer. A series of moving object images frozen by flash pulses, carried information of moving object, are recorded by a CCD video camera, and result images are sent to a computer to be frozen, recognized and processed with special hardware and software. Obtained parameters can be displayed, output as remote controlling signals or written into CD. The highest videography frequency is 30,000 images per second. The shortest image freezing time is several microseconds. The system has been applied to wide fields of energy, chemistry, medicine, biological engineering, aero- dynamics, explosion, multi-phase flow, mechanics, vibration, athletic training, weapon development and national defense engineering. It can also be used in production streamline to carry out the online, real-time monitoring and controlling.

Digital mammography with high-resolution storage plates (CR) versus full-field digital mammography (CCD) (DR) for microcalcifications and focal lesions - a retrospective clinical histologic analysis (n = 102)

International Nuclear Information System (INIS)

Schulz-Wendtland, R.; Lell, M.; Wenkel, E.; Boehner, C.; Dassel, M.S.; Bautz, W.

2005-01-01

Purpose: to determine the diagnostic accuracy of microcalcifications and focal lesions in a retrospective clinical-histological study using high-resolution digital phosphor storage plates (hard copy) and full-field digital mammography (hard copy). Materials and methods: from May 2003 to September 2003, 102 patients underwent digital storage plate mammography (CR), using a mammography unit (Mammomat 3000 N, Siemens) in combination with a high resolution (9 lp/mm) digital storage phosphor plate system (pixel size 50 μm) (Fuji/Siemens). After diagnosis and preoperative wire localization, full-field digital mammography (CCD) (DR) was performed with the same exposure parameters. The full-field digital mammography used a CCD-detector (SenoScan) (fisher imaging) with a resolution of 10 Ip/mm and a pixel size of 50 μm. Five investigators determined the diagnosis (BI-RADS trademark I-V) retrospectively after the operation from randomly distributed mediolateral views (hard copy reading). These results were correlated with the final histology. Results: the diagnostic accuracy of digital storage plate mammography (CR) and full-field digital mammography (CCD) (DR) was 73% and 71% for all findings (n = 102), 73% and 71% for microcalcifications (n = 51), and 72% and 70% for focal lesions (n = 51). The overall results showed no difference. Conclusion: our findings indicate the equivalence of high-resolution digital phosphor storage plate mammography (CR) and full-field digital mammography (CCD) (DR). (orig.)

New Design Concept for Universal CCD Controller

Directory of Open Access Journals (Sweden)

Wonyong Han

1994-06-01

Full Text Available Currently, the CCDs are widely used in astronomical observations either in direct imaging use or spectroscopic mode. However according to the recent technical advances, new large format CCDs are rapidly developed which have better performances with higher quantum efficiency and sensitivity. In many cases, some microprocessors have been adopted to deal with necessary digital logic for a CCD imaging system. This could often lack the flexibility of a system for a user to upgrade with new devices, especially of it is a commercial product. A new design concept has been explored which could provide the opportunity to deal with any format of devices from ant manufactures effectively for astronomical purposes. Recently available PLD (Programmable Logic Devices technology makes it possible to develop such digital circuit design, which can be integrated into a single component, instead of using microprocessors. The design concept could dramatically increase the efficiency and flexibility of a CCD imaging system, particularly when new or large format devices are available and to upgrade the performance of a system. Some variable system control parameters can be selected by a user with a wider range of choice. The software can support such functional requirements very conveniently. This approach can be applied not only to astronomical purpose, but also to some related fields, such as remote sensing and industrial applications.

A programmable CCD driver circuit for multiphase CCD operation

International Nuclear Information System (INIS)

Ewin, A.J.; Reed, K.V.

1989-01-01

A programmable CCD driver circuit was designed to drive CCD's in multiphased modes. The purpose of the drive electronics was to operate developmental CCD imaging arrays for NASA's Moderate Resolution Imaging Spectrometer - Tiltable (MODIS-T). Five prototype arrays were designed. Valid's Graphics Editor (GED) was used to design the driver. With this driver design, any of the five arrays can be readout. Designing the driver with GED allowed functional simulation, timing verification, and certain packaging analyses to be done on the design before fabrication. The driver verified its function with the master clock running up to 10 MHz. This suggests a maximum rate of 400 Kpixels/sec. Timing and packaging parameters were verified. the design uses 54 TTL component chips

Solar Mosaic Inc. Mosaic Home Solar Loan SunShot 9 Final Report

Energy Technology Data Exchange (ETDEWEB)

Walsh, Colin James [Solar Mosaic Inc., Oakland, CA (United States)

2017-02-09

The 6686 Mosaic SunShot award has helped Solar Mosaic Inc to progress from an early stage startup focused on commercial crowdfunding to a leading multi-state residential solar lender. The software platform is now used by the majority of the nation's top solar installers and offers a variety of simple home solar loans. Mosaic is has originated approximately $1Bil in solar loans to date to put solar on over 35k rooftops. The company now lends to homeowners with a wide range of credit scores across multiple states and mitigates boundaries preventing them from profiting from ownership of a home solar system. The project included milestones in 5 main categories: 1. Lending to homeowners outside of CA 2. Lending to homeowners with FICO scores under 700 3. Packaging O&M with the home solar loan 4. Allowing residential installers to process home solar loans via API 5. Lowering customer acquisition costs below $1500 This report includes a detailed review of the final results achieved and key findings.

First observations from a CCD all-sky spectrograph at Barentsburg (Spitsbergen

Directory of Open Access Journals (Sweden)

S. A. Chernouss

2008-05-01

Full Text Available A digital CCD all-sky spectrograph was made by the Polar Geophysical Institute (PGI to support IPY activity in auroral research. The device was tested at the Barentsburg observatory of PGI during the winter season of 2005–2006. The spectrograph is based on a cooled CCD and a transmission grating. The main features of this spectrograph are: a wide field of view (~180°, a wide spectral range (380–740 nm, a spectral resolution of 0.6 nm, a background level of about 100 R at 1-min exposure time. Several thousand spectra of nightglow and aurora were recorded during the observation season. It was possible to register both the strong auroral emissions, as well as weak ones. Spectra of aurora, including nitrogen and oxygen molecular and atomic emissions, as well as OH emissions of the nightglow are shown. A comparison has been conducted of auroral spectra obtained by the film all-sky spectral camera C-180-S at Spitsbergen during IGY, with spectra obtained at Barentsburg during the last winter season. The relationship between the red (630.0 nm and green (557.7 nm auroral emissions shows that the green emission is dominant near the minimum of the solar cycle activity (2005–2006. The opposite situation is observed during 1958–1959, with a maximum solar cycle activity.

Association between the degree of mosaicism and the severity of syndrome in Turner mosaics and Klinefelter mosaics.

Science.gov (United States)

Sarkar, R; Marimuthu, K M

1983-12-01

This study, based on the investigations carried on 82 cases of Turners of which 50 of them were mosaics and 85 cases of Klinefelters of which 70 of them were mosaics, is an attempt to explain the vast range of clinical variations observed in cytogenetically established Turner mosaics (45,X/46,XX) and Klinefelter mosaics (47,XXY/46,XY) in the light of the degree of mosaicism present in them. It was observed that the severity of the syndrome in Turner mosaics and Klinefelter mosaics increased with the relative increase in the abnormal cell line population.

The OmegaCAM 16K x 16K CCD detector system for the ESO VLT Survey Telescope (VST)

Science.gov (United States)

Iwert, Olaf; Baade, D.; Balestra, A.; Baruffolo, A.; Bortolussi, A.; Christen, F.; Cumani, C.; Deiries, S.; Downing, M.; Geimer, C.; Hess, G.; Hess, J.; Kuijken, K.; Lizon, J.; Muschielok, B.; Nicklas, H.; Reiss, R.; Reyes, J.; Silber, A.; Thillerup, J.; Valentijn, E.

2006-06-01

A 16K x 16K, 1 degree x 1 degree field, detector system was developed by ESO for the OmegaCAM instrument for use on the purpose built ESO VLT Survey Telescope (VST). The focal plane consists of an 8 x 4 mosaic of 2K x 4K 15um pixel e2v CCDs and four 2K x 4K CCDs on the periphery for the opto-mechanical control of the telescope. The VST is a single instrument telescope. This placed stringent reliability requirements on the OmegaCAM detector system such as 10 years lifetime and maximum downtime of 1.5 %. Mounting at Cassegrain focus required a highly autonomous self-contained cooling system that could deliver 65 W of cooling power. Interface space for the detector head was severely limited by the way the instrument encloses the CCD cryostat. The detector system features several novel ideas tailored to meet these requirements and described in this paper: Key design drivers of the detector head were the easily separable but precisely aligned connections to the optical field flattener on the top and the cooling system at the bottom. Material selection, surface treatment, specialized coatings and in-situ plasma cleaning were crucial to prevent contamination of the detectors. Inside the cryostat, cryogenic and electrical connections were disentangled to keep the configuration modular, integration friendly and the detectors in a safe condition during all mounting steps. A compact unit for logging up to 125 Pt100 temperature sensors and associated thermal control loops was developed (ESO's new housekeeping unit PULPO 2), together with several new modular Pt100 packaging and mounting concepts. The electrical grouping of CCDs based on process parameters and test results is explained. Three ESO standardized FIERA CCD controllers in different configurations are used. Their synchronization mechanism for read-out is discussed in connection with the CCD grouping scheme, the shutter, and the integrated guiding and image analysis facility with four independent 2K x 4K CCDs. An

Development and prequalification of a medium-accuracy wide-field pattern recognition starsensor

Science.gov (United States)

Verhoeff, Peter; de Boom, Kees; Meier, H.; Andre, G.

1993-10-01

A development of a medium accuracy, CCD based starsensor, intended for use on the new generation of communication satellites is in progress. The sensor aims at an accuracy of 0.06 degree(s) in a FOV of 30 degree(s) X 40 degree(s). The sensor is designed to detect stars with a brightness of mvia EQM is ongoing now. Intended to be versatile in applications for telecommunication and other missions, a unit is developed which is adaptive and has low recurring cost. The PRS concept is based on new MPP CCD technology. This technology brings passive thermal control without Peltier elements within reach. The electronics accommodated inside the PRS sensorheads is miniaturized by large scale integration by using a Field Programmable Gate Array (FPGA) and Surface Mount Technology (SMT). For cost reasons no microprocessor was implemented. This paper describes the main design features of the PRS, the selection and testing of appropriate CCD, performance characteristics of the system and test results obtained from the PRS breadboard system.

Efisiensi Tular Benih Squash mosaic virus pada Cucurbitaceae

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Susanti Mugi Lestari

2014-09-01

Full Text Available Infection of viruses on Cucurbitaceae may cause high yield and economic losses. Squash mosaic virus is a seed borne virus and among the most important virus infecting Cucurbitaceae. The aims of these research was to detect infection of several viruses on Cucurbitaceae and to examine seed transmission efficiency of SqMV. Detection of Cucumber mosaic virus (CMV, Squash mosaic virus (SqMV, Watermelon mosaic virus-2 (WMV-2, Zucchini yellow mosaic virus (ZYMV, and Tobacco ringspot virus (TRSV from field samples and seeds was conducted using Indirect-ELISA method. Infection of CMV, SqMV and ZYMV was detected from field samples. Seed transmission of SqMV on commercial seeds of bottle gourd, watermelon, zucchini, cabocha, cucumber, and melon was 13, 13, 33, 73, 100, and 100%, respectively. Seed transmission of ZYMV was only occurred on bottle gourd and zucchini, i.e. 13.3% and 26.67%, respectively. Infection of SqMV through F2 seed was determined from cucumber, bottle gourd, and melon, i.e. 93, 100, and 100%, respectively. Therefore, the status of SqMV as quarantine pest should be evaluated since SqMV was already found in West Java.

3D photo mosaicing of Tagiri shallow vent field by an autonomous underwater vehicle (3rd report) - Mosaicing method based on navigation data and visual features -

Science.gov (United States)

Maki, Toshihiro; Ura, Tamaki; Singh, Hanumant; Sakamaki, Takashi

Large-area seafloor imaging will bring significant benefits to various fields such as academics, resource survey, marine development, security, and search-and-rescue. The authors have proposed a navigation method of an autonomous underwater vehicle for seafloor imaging, and verified its performance through mapping tubeworm colonies with the area of 3,000 square meters using the AUV Tri-Dog 1 at Tagiri vent field, Kagoshima bay in Japan (Maki et al., 2008, 2009). This paper proposes a post-processing method to build a natural photo mosaic from a number of pictures taken by an underwater platform. The method firstly removes lens distortion, invariances of color and lighting from each image, and then ortho-rectification is performed based on camera pose and seafloor estimated by navigation data. The image alignment is based on both navigation data and visual characteristics, implemented as an expansion of the image based method (Pizarro et al., 2003). Using the two types of information realizes an image alignment that is consistent both globally and locally, as well as making the method applicable to data sets with little visual keys. The method was evaluated using a data set obtained by the AUV Tri-Dog 1 at the vent field in Sep. 2009. A seamless, uniformly illuminated photo mosaic covering the area of around 500 square meters was created from 391 pictures, which covers unique features of the field such as bacteria mats and tubeworm colonies.

A New CCD Camera at the Molėtai Observatory

Directory of Open Access Journals (Sweden)

Zdanavičius J.

2003-12-01

Full Text Available The results of the first testing of a new CCD camera of the Molėtai Observatory are given. The linearity and the flat field corrections of good accuracy are determined by using shifted star field exposures.

Wide-Field Imaging Using Nitrogen Vacancies

Science.gov (United States)

Englund, Dirk Robert (Inventor); Trusheim, Matthew Edwin (Inventor)

2017-01-01

Nitrogen vacancies in bulk diamonds and nanodiamonds can be used to sense temperature, pressure, electromagnetic fields, and pH. Unfortunately, conventional sensing techniques use gated detection and confocal imaging, limiting the measurement sensitivity and precluding wide-field imaging. Conversely, the present sensing techniques do not require gated detection or confocal imaging and can therefore be used to image temperature, pressure, electromagnetic fields, and pH over wide fields of view. In some cases, wide-field imaging supports spatial localization of the NVs to precisions at or below the diffraction limit. Moreover, the measurement range can extend over extremely wide dynamic range at very high sensitivity.

The astro-geodetic use of CCD for gravity field refinement

Science.gov (United States)

Gerstbach, G.

1996-07-01

The paper starts with a review of geoid projects, where vertical deflections are more effective than gravimetry. In alpine regions the economy of astrogeoids is at least 10 times higher, but many countries do not make use of this fact - presumably because the measurements are not fully automated up to now. Based upon the experiences of astrometry of high satellites and own tests the author analyses the use of CCD for astro-geodetic measurements. Automation and speeding up will be possible in a few years, the latter depending on the observation scheme. Sensor characteristics, cooling and reading out of the devices should be harmonized. Using line sensors in small prism astrolabes, the CCD accuracy will reach the visual one (±0.2″) within 5-10 years. Astrogeoids can be combined ideally with geological data, because vertical variation of rock densities does not cause systematic effects (contrary to gravimetry). So a geoid of ±5 cm accuracy (achieved in Austria and other alpine countries by 5-10 points per 1000 km 2) can be improved to ±2 cm without additional observations and border effects.

An optical test bench for the precision characterization of absolute quantum efficiency for the TESS CCD detectors

International Nuclear Information System (INIS)

Krishnamurthy, A.; Villasenor, J.; Kissel, S.; Ricker, G.; Vanderspek, R.

2017-01-01

The Transiting Exoplanet Survey Satellite (TESS) will search for planets transiting bright stars with Ic ∼< 13. TESS has been selected by NASA for launch in 2018 as an Astrophysics Explorer mission, and is expected to discover a thousand or more planets that are smaller in size than Neptune. TESS will employ four wide-field optical charge-coupled device (CCD) cameras with a band-pass of 650 nm–1050 nm to detect temporary drops in brightness of stars due to planetary transits. The 1050 nm limit is set by the quantum efficiency (QE) of the CCDs. The detector assembly consists of four back-illuminated MIT Lincoln Laboratory CCID-80 devices. Each CCID-80 device consists of 2048×2048 imaging array and 2048×2048 frame store regions. Very precise on-ground calibration and characterization of CCD detectors will significantly assist in the analysis of the science data obtained in space. The characterization of the absolute QE of the CCD detectors is a crucial part of the characterization process because QE affects the performance of the CCD significantly over the redder wavelengths at which TESS will be operating. An optical test bench with significantly high photometric stability has been developed to perform precise QE measurements. The design of the test setup along with key hardware, methodology, and results from the test campaign are presented.

Michelson wide-field stellar interferometry

NARCIS (Netherlands)

Montilla, I.

2004-01-01

The main goal of this thesis is to develop a system to permit wide field operation of Michelson Interferometers. A wide field of view is very important in applications such as the observation of extended or multiple objects, the fringe acquisition and/ or tracking on a nearby unresolved object, and

Enhanced performance CCD output amplifier

Science.gov (United States)

Dunham, Mark E.; Morley, David W.

1996-01-01

A low-noise FET amplifier is connected to amplify output charge from a che coupled device (CCD). The FET has its gate connected to the CCD in common source configuration for receiving the output charge signal from the CCD and output an intermediate signal at a drain of the FET. An intermediate amplifier is connected to the drain of the FET for receiving the intermediate signal and outputting a low-noise signal functionally related to the output charge signal from the CCD. The amplifier is preferably connected as a virtual ground to the FET drain. The inherent shunt capacitance of the FET is selected to be at least equal to the sum of the remaining capacitances.

Crystallization of the C-terminal domain of the addiction antidote CcdA in complex with its toxin CcdB

International Nuclear Information System (INIS)

Buts, Lieven; De Jonge, Natalie; Loris, Remy; Wyns, Lode; Dao-Thi, Minh-Hoa

2005-01-01

The CcdA C-terminal domain was crystallized in complex with CcdB in two crystal forms that diffract to beyond 2.0 Å resolution. CcdA and CcdB are the antidote and toxin of the ccd addiction module of Escherichia coli plasmid F. The CcdA C-terminal domain (CcdA C36 ; 36 amino acids) was crystallized in complex with CcdB (dimer of 2 × 101 amino acids) in three different crystal forms, two of which diffract to high resolution. Form II belongs to space group P2 1 2 1 2 1 , with unit-cell parameters a = 37.6, b = 60.5, c = 83.8 Å and diffracts to 1.8 Å resolution. Form III belongs to space group P2 1 , with unit-cell parameters a = 41.0, b = 37.9, c = 69.6 Å, β = 96.9°, and diffracts to 1.9 Å resolution

Real-time image mosaicing for medical applications.

Science.gov (United States)

Loewke, Kevin E; Camarillo, David B; Jobst, Christopher A; Salisbury, J Kenneth

2007-01-01

In this paper we describe the development of a robotically-assisted image mosaicing system for medical applications. The processing occurs in real-time due to a fast initial image alignment provided by robotic position sensing. Near-field imaging, defined by relatively large camera motion, requires translations as well as pan and tilt orientations to be measured. To capture these measurements we use 5-d.o.f. sensing along with a hand-eye calibration to account for sensor offset. This sensor-based approach speeds up the mosaicing, eliminates cumulative errors, and readily handles arbitrary camera motions. Our results have produced visually satisfactory mosaics on a dental model but can be extended to other medical images.

Transmission electron microscope CCD camera

Science.gov (United States)

Downing, Kenneth H.

1999-01-01

In order to improve the performance of a CCD camera on a high voltage electron microscope, an electron decelerator is inserted between the microscope column and the CCD. This arrangement optimizes the interaction of the electron beam with the scintillator of the CCD camera while retaining optimization of the microscope optics and of the interaction of the beam with the specimen. Changing the electron beam energy between the specimen and camera allows both to be optimized.

A fluorescent screen + CCD system for quality assurance of therapeutic scanned ion beams

Energy Technology Data Exchange (ETDEWEB)

Takeshita, E., E-mail: eriuli@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Furukawa, T., E-mail: t_furu@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Inaniwa, T., E-mail: taku@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Sato, S., E-mail: shin_s@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Himukai, T., E-mail: himukai@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Shirai, T., E-mail: t_shirai@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Noda, K., E-mail: noda_k@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan)

2011-12-15

A fluorescent screen + a charge coupled device (CCD) system were developed to verify the performance of scanned ion beams at the HIMAC. The fluorescent light from the screen is observed by the CCD camera. Two-dimensional fields, produced by the scanning process, i.e., the position and the size of the beam for each scan, represent of the important issues in scanning irradiation. In the developed system, the two-dimensional relative fluence and the flatness of the irradiation field were measured in a straightforward technique from the luminance distribution on the screen. The position and the size of the beams were obtained from centroid computation results of the brightness. By the good sensitivity and spatial resolution of the fluorescent screen + CCD system, the scanned ion beams were verified as the measurements at the HIMAC prototype scanning system.

A fluorescent screen + CCD system for quality assurance of therapeutic scanned ion beams

Science.gov (United States)

Takeshita, E.; Furukawa, T.; Inaniwa, T.; Sato, S.; Himukai, T.; Shirai, T.; Noda, K.

2011-12-01

A fluorescent screen + a charge coupled device (CCD) system were developed to verify the performance of scanned ion beams at the HIMAC. The fluorescent light from the screen is observed by the CCD camera. Two-dimensional fields, produced by the scanning process, i.e., the position and the size of the beam for each scan, represent of the important issues in scanning irradiation. In the developed system, the two-dimensional relative fluence and the flatness of the irradiation field were measured in a straightforward technique from the luminance distribution on the screen. The position and the size of the beams were obtained from centroid computation results of the brightness. By the good sensitivity and spatial resolution of the fluorescent screen + CCD system, the scanned ion beams were verified as the measurements at the HIMAC prototype scanning system.

Application of X-ray CCD camera in X-ray spot diagnosis of rod-pinch diode

International Nuclear Information System (INIS)

Song Yan; Zhou Ming; Song Guzhou; Ma Jiming; Duan Baojun; Han Changcai; Yao Zhiming

2015-01-01

The pinhole imaging technique is widely used in the measurement of X-ray spot of rod-pinch diode. The X-ray CCD camera, which was composed of film, fiber optic taper and CCD camera, was employed to replace the imaging system based on scintillator, lens and CCD camera in the diagnosis of X-ray spot. The resolution of the X-ray CCD camera was studied. The resolution is restricted by the film and is 5 lp/mm in the test with Pb resolution chart. The frequency is 1.5 lp/mm when the MTF is 0.5 in the test with edge image. The resolution tests indicate that the X-ray CCD camera can meet the requirement of the diagnosis of X-ray spot whose scale is about 1.5 mm when the pinhole imaging magnification is 0.5. At last, the image of X-ray spot was gained and the restoration was implemented in the diagnosis of X-ray spot of rod-pinch diode. (authors)

CCD research. [design, fabrication, and applications

Science.gov (United States)

Gassaway, J. D.

1976-01-01

The fundamental problems encountered in designing, fabricating, and applying CCD's are reviewed. Investigations are described and results and conclusions are given for the following: (1) the development of design analyses employing computer aided techniques and their application to the design of a grapped structure; (2) the role of CCD's in applications to electronic functions, in particular, signal processing; (3) extending the CCD to silicon films on sapphire (SOS); and (4) all aluminum transfer structure with low noise input-output circuits. Related work on CCD imaging devices is summarized.

Infeksi Cucumber mosaic virus dan Chilli veinal mottle virus pada Cabai di Kabupaten Rejang Lebong, Bengkulu

Directory of Open Access Journals (Sweden)

Mimi Sutrawati

2013-04-01

Full Text Available Mosaic disease caused by Cucumber mosaic virus (CMV and Chilli veinal mottle Virus (ChiVMV has been distributed widely in chilli in Indonesia and considered as important disease. A research was conducted to investigate the spread and incidence of CMV and ChiVMV in Rejang Lebong, Bengkulu and to identify its insect vector. Symptomatic and asymptomatic leaf samples were collected systematically from several chillipepper fields for further detection by DAS-ELISA (Double antibody sandwich-enzyme linked immunosorbant assay using specific antibody for CMV and ChiVMV. The result showed that infection of both CMV and ChiVMV was found with disease incidence reached 20-50%, whereas infection only by ChiVMV or CMV were 50-80% and 20-50%, respectively. One species of aphid, i.e. Aphis gossypii was found from the fields.Key words: Aphis gossypii, CMV, ChiVMV, disease incidence

Status of cassava mosaic disease and whitefly population in Zambia ...

African Journals Online (AJOL)

Cassava mosaic disease is the most important disease affecting cassava in Zambia. A study was conducted through a survey to determine the status of cassava mosaic disease incidence, severity and whitefly abundance in farmers' fields in six provinces: Lusaka, Northern, North-Western, Luapula, Eastern and Western ...

Female chromosome X mosaicism is age-related and preferentially affects the inactivated X chromosome

Science.gov (United States)

Machiela, Mitchell J.; Zhou, Weiyin; Karlins, Eric; Sampson, Joshua N.; Freedman, Neal D.; Yang, Qi; Hicks, Belynda; Dagnall, Casey; Hautman, Christopher; Jacobs, Kevin B.; Abnet, Christian C.; Aldrich, Melinda C.; Amos, Christopher; Amundadottir, Laufey T.; Arslan, Alan A.; Beane-Freeman, Laura E.; Berndt, Sonja I.; Black, Amanda; Blot, William J.; Bock, Cathryn H.; Bracci, Paige M.; Brinton, Louise A.; Bueno-de-Mesquita, H Bas; Burdett, Laurie; Buring, Julie E.; Butler, Mary A.; Canzian, Federico; Carreón, Tania; Chaffee, Kari G.; Chang, I-Shou; Chatterjee, Nilanjan; Chen, Chu; Chen, Constance; Chen, Kexin; Chung, Charles C.; Cook, Linda S.; Crous Bou, Marta; Cullen, Michael; Davis, Faith G.; De Vivo, Immaculata; Ding, Ti; Doherty, Jennifer; Duell, Eric J.; Epstein, Caroline G.; Fan, Jin-Hu; Figueroa, Jonine D.; Fraumeni, Joseph F.; Friedenreich, Christine M.; Fuchs, Charles S.; Gallinger, Steven; Gao, Yu-Tang; Gapstur, Susan M.; Garcia-Closas, Montserrat; Gaudet, Mia M.; Gaziano, J. Michael; Giles, Graham G.; Gillanders, Elizabeth M.; Giovannucci, Edward L.; Goldin, Lynn; Goldstein, Alisa M.; Haiman, Christopher A.; Hallmans, Goran; Hankinson, Susan E.; Harris, Curtis C.; Henriksson, Roger; Holly, Elizabeth A.; Hong, Yun-Chul; Hoover, Robert N.; Hsiung, Chao A.; Hu, Nan; Hu, Wei; Hunter, David J.; Hutchinson, Amy; Jenab, Mazda; Johansen, Christoffer; Khaw, Kay-Tee; Kim, Hee Nam; Kim, Yeul Hong; Kim, Young Tae; Klein, Alison P.; Klein, Robert; Koh, Woon-Puay; Kolonel, Laurence N.; Kooperberg, Charles; Kraft, Peter; Krogh, Vittorio; Kurtz, Robert C.; LaCroix, Andrea; Lan, Qing; Landi, Maria Teresa; Marchand, Loic Le; Li, Donghui; Liang, Xiaolin; Liao, Linda M.; Lin, Dongxin; Liu, Jianjun; Lissowska, Jolanta; Lu, Lingeng; Magliocco, Anthony M.; Malats, Nuria; Matsuo, Keitaro; McNeill, Lorna H.; McWilliams, Robert R.; Melin, Beatrice S.; Mirabello, Lisa; Moore, Lee; Olson, Sara H.; Orlow, Irene; Park, Jae Yong; Patiño-Garcia, Ana; Peplonska, Beata; Peters, Ulrike; Petersen, Gloria M.; Pooler, Loreall; Prescott, Jennifer; Prokunina-Olsson, Ludmila; Purdue, Mark P.; Qiao, You-Lin; Rajaraman, Preetha; Real, Francisco X.; Riboli, Elio; Risch, Harvey A.; Rodriguez-Santiago, Benjamin; Ruder, Avima M.; Savage, Sharon A.; Schumacher, Fredrick; Schwartz, Ann G.; Schwartz, Kendra L.; Seow, Adeline; Wendy Setiawan, Veronica; Severi, Gianluca; Shen, Hongbing; Sheng, Xin; Shin, Min-Ho; Shu, Xiao-Ou; Silverman, Debra T.; Spitz, Margaret R.; Stevens, Victoria L.; Stolzenberg-Solomon, Rachael; Stram, Daniel; Tang, Ze-Zhong; Taylor, Philip R.; Teras, Lauren R.; Tobias, Geoffrey S.; Van Den Berg, David; Visvanathan, Kala; Wacholder, Sholom; Wang, Jiu-Cun; Wang, Zhaoming; Wentzensen, Nicolas; Wheeler, William; White, Emily; Wiencke, John K.; Wolpin, Brian M.; Wong, Maria Pik; Wu, Chen; Wu, Tangchun; Wu, Xifeng; Wu, Yi-Long; Wunder, Jay S.; Xia, Lucy; Yang, Hannah P.; Yang, Pan-Chyr; Yu, Kai; Zanetti, Krista A.; Zeleniuch-Jacquotte, Anne; Zheng, Wei; Zhou, Baosen; Ziegler, Regina G.; Perez-Jurado, Luis A.; Caporaso, Neil E.; Rothman, Nathaniel; Tucker, Margaret; Dean, Michael C.; Yeager, Meredith; Chanock, Stephen J.

2016-01-01

To investigate large structural clonal mosaicism of chromosome X, we analysed the SNP microarray intensity data of 38,303 women from cancer genome-wide association studies (20,878 cases and 17,425 controls) and detected 124 mosaic X events >2 Mb in 97 (0.25%) women. Here we show rates for X-chromosome mosaicism are four times higher than mean autosomal rates; X mosaic events more often include the entire chromosome and participants with X events more likely harbour autosomal mosaic events. X mosaicism frequency increases with age (0.11% in 50-year olds; 0.45% in 75-year olds), as reported for Y and autosomes. Methylation array analyses of 33 women with X mosaicism indicate events preferentially involve the inactive X chromosome. Our results provide further evidence that the sex chromosomes undergo mosaic events more frequently than autosomes, which could have implications for understanding the underlying mechanisms of mosaic events and their possible contribution to risk for chronic diseases. PMID:27291797

Wide-field fundus autofluorescence corresponds to visual fields in chorioretinitis patients

Directory of Open Access Journals (Sweden)

Seidensticker F

2011-11-01

Full Text Available Florian Seidensticker1, Aljoscha S Neubauer1, Tamer Wasfy1,2, Carmen Stumpf1, Stephan R Thurau1,*, Anselm Kampik1, Marcus Kernt1,*1Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany; 2Department of Ophthalmology, Tanta University, Tanta, Egypt *Both authors contributed equally to this workBackground and objectives: Detection of peripheral fundus autofluorescence (FAF using conventional scanning laser ophthalmoscopes (SLOs is difficult and requires pupil dilation. Here we evaluated the diagnostic properties of wide-field FAF detected by a two-laser wavelength wide-field SLO in uveitis patients.Study design/materials and methods: Observational case series of four patients suffering from different types of posterior uveitis/chorioretinitis. Wide-field FAF images were compared to visual fields. Panretinal FAF was detected by a newly developed SLO, which allows FAF imaging of up to 200° of the retina in one scan without the need for pupil dilation. Visual fields were obtained by Goldmann manual perimetry.Results: Findings from wide-field FAF imaging showed correspondence to visual field defects in all cases.Conclusion: Wide-field FAF allowed the detection of visual field defect-related alterations of the retinal pigment epithelium in all four uveitis cases.Keywords: fundus autofluorescence (FAF, Optomap, wide-field scanning laser ophthalmoscopy, imaging, uveitis, visual field

Flat Field Anomalies in an X-Ray CCD Camera Measured Using a Manson X-Ray Source

International Nuclear Information System (INIS)

Michael Haugh

2008-01-01

The Static X-ray Imager (SXI) is a diagnostic used at the National Ignition Facility (NIF) to measure the position of the X-rays produced by lasers hitting a gold foil target. It determines how accurately NIF can point the laser beams and is critical to proper NIF operation. Imagers are located at the top and the bottom of the NIF target chamber. The CCD chip is an X-ray sensitive silicon sensor, with a large format array (2k x 2k), 24 (micro)m square pixels, and 15 (micro)m thick. A multi-anode Manson X-ray source, operating up to 10kV and 2mA, was used to characterize and calibrate the imagers. The output beam is heavily filtered to narrow the spectral beam width, giving a typical resolution E/ΔE ∼ 12. The X-ray beam intensity was measured using an absolute photodiode that has accuracy better than 1% up to the Si K edge and better than 5% at higher energies. The X-ray beam provides full CCD illumination and is flat, within ±1.5% maximum to minimum. The spectral efficiency was measured at 10 energy bands ranging from 930 eV to 8470 eV. The efficiency pattern follows the properties of Si. The maximum quantum efficiency is 0.71. We observed an energy dependent pixel sensitivity variation that showed continuous change over a large portion of the CCD. The maximum sensitivity variation was >8% at 8470 eV. The geometric pattern did not change at lower energies, but the maximum contrast decreased and was less than the measurement uncertainty below 4 keV. We were also able to observe debris on the CCD chip. The debris showed maximum contrast at the lowest energy used, 930 eV, and disappeared by 4 keV. The Manson source is a powerful tool for characterizing the imaging errors of an X-ray CCD imager. These errors are quite different from those found in a visible CCD imager

Multiband CCD Image Compression for Space Camera with Large Field of View

Directory of Open Access Journals (Sweden)

Jin Li

2014-01-01

Full Text Available Space multiband CCD camera compression encoder requires low-complexity, high-robustness, and high-performance because of its captured images information being very precious and also because it is usually working on the satellite where the resources, such as power, memory, and processing capacity, are limited. However, the traditional compression approaches, such as JPEG2000, 3D transforms, and PCA, have the high-complexity. The Consultative Committee for Space Data Systems-Image Data Compression (CCSDS-IDC algorithm decreases the average PSNR by 2 dB compared with JPEG2000. In this paper, we proposed a low-complexity compression algorithm based on deep coupling algorithm among posttransform in wavelet domain, compressive sensing, and distributed source coding. In our algorithm, we integrate three low-complexity and high-performance approaches in a deeply coupled manner to remove the spatial redundant, spectral redundant, and bit information redundancy. Experimental results on multiband CCD images show that the proposed algorithm significantly outperforms the traditional approaches.

Study of x-ray CCD image sensor and application

Science.gov (United States)

Wang, Shuyun; Li, Tianze

2008-12-01

In this paper, we expounded the composing, specialty, parameter, its working process, key techniques and methods for charge coupled devices (CCD) twice value treatment. Disposal process for CCD video signal quantification was expatiated; X-ray image intensifier's constitutes, function of constitutes, coupling technique of X-ray image intensifier and CCD were analyzed. We analyzed two effective methods to reduce the harm to human beings when X-ray was used in the medical image. One was to reduce X-ray's radiation and adopt to intensify the image penetrated by X-ray to gain the same effect. The other was to use the image sensor to transfer the images to the safe area for observation. On this base, a new method was presented that CCD image sensor and X-ray image intensifier were combined organically. A practical medical X-ray photo electricity system was designed which can be used in the records and time of the human's penetrating images. The system was mainly made up with the medical X-ray, X-ray image intensifier, CCD vidicon with high resolution, image processor, display and so on. Its characteristics are: change the invisible X-ray into the visible light image; output the vivid images; short image recording time etc. At the same time we analyzed the main aspects which affect the system's resolution. Medical photo electricity system using X-ray image sensor can reduce the X-ray harm to human sharply when it is used in the medical diagnoses. At last we analyzed and looked forward the system's application in medical engineering and the related fields.

A large area cooled-CCD detector for electron microscopy

International Nuclear Information System (INIS)

Faruqi, A.R.; Andrews, H.N.; Raeburn, C.

1994-01-01

Large area cooled-CCDs are an excellent medium for (indirectly) recording electron images and electron diffraction patterns in real time and for use in electron tomography; real-time imaging is extremely useful in making rapid adjustments in the electron microscope. CCDs provide high sensitivity (useful for minimising dosage to radiation-sensitive biological specimen), good resolution, stable performance, excellent dynamic range and linearity and a reasonably fast readout.We have built an electron imaging device based on the EEV 1152 by 814 pixel CCD which is controlled from a unix based SUN Sparcstation operating under X-Windows. The incident 100 kV electrons are converted to visible light in a 0.5 mm thick YAG single crystal which is imaged through a lens on to the CCD.The CCD electronics is designed to be as flexible as possible and allows a wide variation in the readout speed to cater for the relatively fast application where readout noise is less critical and low readout noise applications where the extra few seconds of readout time are not significant. The CCD electronics is built in VME format which is controlled through a S-bus to VME driver. With two parallel channels of readout the whole image can be read out in similar 1 s (using the faster readout speed) with 16 bit precision and the image is displayed under X-Windows in a few seconds. The present readout works at 500 kHz and has a noise of similar 30 e rms per pixel. With a Peltier cooling device we can operate the CCD at similar -40 circle C which reduces the dark current adequately to allow exposures of up to several minutes. Several examples of patterns collected with the system on a Philips CM12 microscope will be presented. ((orig.))

Visualization and interaction tools for aerial photograph mosaics

Science.gov (United States)

Fernandes, João Pedro; Fonseca, Alexandra; Pereira, Luís; Faria, Adriano; Figueira, Helder; Henriques, Inês; Garção, Rita; Câmara, António

1997-05-01

This paper describes the development of a digital spatial library based on mosaics of digital orthophotos, called Interactive Portugal, that will enable users both to retrieve geospatial information existing in the Portuguese National System for Geographic Information World Wide Web server, and to develop local databases connected to the main system. A set of navigation, interaction, and visualization tools are proposed and discussed. They include sketching, dynamic sketching, and navigation capabilities over the digital orthophotos mosaics. Main applications of this digital spatial library are pointed out and discussed, namely for education, professional, and tourism markets. Future developments are considered. These developments are related to user reactions, technological advancements, and projects that also aim at delivering and exploring digital imagery on the World Wide Web. Future capabilities for site selection and change detection are also considered.

VizieR Online Data Catalog: Proper motions and photometry of stars in NGC 3201 (Sariya+, 2017)

Science.gov (United States)

Sariya, D. P.; Jiang, I.-G.; Yadav, R. K. S.

2017-07-01

To determine the PMs of the stars in this work, we used archive images (http://archive.eso.org/eso/esoarchivemain.html) from observations made with the 2.2m ESO/MPI telescope at La Silla, Chile. This telescope contains a mosaic camera called the Wide-Field Imager (WFI), consisting of 4*2 (i.e., 8 CCD chips). Since each CCD has an array of 2048*4096 pixels, WFI ultimately produces images with a 34*33arcmin2 field of view. The observational run of the first epoch contains two images in B,V and I bands, each with 240s exposure time observed on 1999 December 05. In the second epoch, we have 35 images with 40s exposure time each in V filter observed during the period of 2014 April 02-05. Thus the epoch gap between the data is ~14.3 years. (2 data files).

Characteristics of rose mosaic diseases

Directory of Open Access Journals (Sweden)

Marek S. Szyndel

2013-12-01

Full Text Available Presented review of rose diseases, associated with the mosaic symptoms, includes common and yellow rose mosaic, rose ring pattern, rose X disease, rose line pattern, yellow vein mosaic and rose mottle mosaic disease. Based on symptomatology and graft transmissibility of causing agent many of those rose disorders are called "virus-like diseases" since the pathogen has never been identified. However, several viruses were detected and identified in roses expressing mosaic symptoms. Currently the most prevalent rose viruses are Prunus necrotic ringspot virus - PNRSV, Apple mosaic virus - ApMV (syn. Rose mosaic virus and Arabis mosaic virus - ArMV Symptoms and damages caused by these viruses are described. Tomato ringspot virus, Tobacco ringspot virus and Rose mottle mosaic virus are also mentioned as rose pa thogcns. Methods of control of rose mosaic diseases are discussed.

Enzymatic study on AtCCD4 and AtCCD7 and their potential to form acyclic regulatory metabolites

KAUST Repository

Bruno, Mark

2016-09-29

The Arabidopsis carotenoid cleavage dioxygenase 4 (AtCCD4) is a negative regulator of the carotenoid content of seeds and has recently been suggested as a candidate for the generation of retrograde signals that are thought to derive from the cleavage of poly-cis-configured carotene desaturation intermediates. In this work, we investigated the activity of AtCCD4 in vitro and used dynamic modeling to determine its substrate preference. Our results document strict regional specificity for cleavage at the C9–C10 double bond in carotenoids and apocarotenoids, with preference for carotenoid substrates and an obstructing effect on hydroxyl functions, and demonstrate the specificity for all-trans-configured carotenes and xanthophylls. AtCCD4 cleaved substrates with at least one ionone ring and did not convert acyclic carotene desaturation intermediates, independent of their isomeric states. These results do not support a direct involvement of AtCCD4 in generating the supposed regulatory metabolites. In contrast, the strigolactone biosynthetic enzyme AtCCD7 converted 9-cis-configured acyclic carotenes, such as 9-cis-ζ-carotene, 9\\'-cis-neurosporene, and 9-cis-lycopene, yielding 9-cis-configured products and indicating that AtCCD7, rather than AtCCD4, is the candidate for forming acyclic retrograde signals.

Enzymatic study on AtCCD4 and AtCCD7 and their potential to form acyclic regulatory metabolites

KAUST Repository

Bruno, Mark; Koschmieder, Julian; Wuest, Florian; Schaub, Patrick; Fehling-Kaschek, Mirjam; Timmer, Jens; Beyer, Peter; Al-Babili, Salim

2016-01-01

The Arabidopsis carotenoid cleavage dioxygenase 4 (AtCCD4) is a negative regulator of the carotenoid content of seeds and has recently been suggested as a candidate for the generation of retrograde signals that are thought to derive from the cleavage of poly-cis-configured carotene desaturation intermediates. In this work, we investigated the activity of AtCCD4 in vitro and used dynamic modeling to determine its substrate preference. Our results document strict regional specificity for cleavage at the C9–C10 double bond in carotenoids and apocarotenoids, with preference for carotenoid substrates and an obstructing effect on hydroxyl functions, and demonstrate the specificity for all-trans-configured carotenes and xanthophylls. AtCCD4 cleaved substrates with at least one ionone ring and did not convert acyclic carotene desaturation intermediates, independent of their isomeric states. These results do not support a direct involvement of AtCCD4 in generating the supposed regulatory metabolites. In contrast, the strigolactone biosynthetic enzyme AtCCD7 converted 9-cis-configured acyclic carotenes, such as 9-cis-ζ-carotene, 9'-cis-neurosporene, and 9-cis-lycopene, yielding 9-cis-configured products and indicating that AtCCD7, rather than AtCCD4, is the candidate for forming acyclic retrograde signals.

Enzymatic study on AtCCD4 and AtCCD7 and their potential to form acyclic regulatory metabolites

Science.gov (United States)

Bruno, Mark; Koschmieder, Julian; Wuest, Florian; Schaub, Patrick; Fehling-Kaschek, Mirjam; Timmer, Jens; Beyer, Peter; Al-Babili, Salim

2016-01-01

The Arabidopsis carotenoid cleavage dioxygenase 4 (AtCCD4) is a negative regulator of the carotenoid content of seeds and has recently been suggested as a candidate for the generation of retrograde signals that are thought to derive from the cleavage of poly-cis-configured carotene desaturation intermediates. In this work, we investigated the activity of AtCCD4 in vitro and used dynamic modeling to determine its substrate preference. Our results document strict regional specificity for cleavage at the C9–C10 double bond in carotenoids and apocarotenoids, with preference for carotenoid substrates and an obstructing effect on hydroxyl functions, and demonstrate the specificity for all-trans-configured carotenes and xanthophylls. AtCCD4 cleaved substrates with at least one ionone ring and did not convert acyclic carotene desaturation intermediates, independent of their isomeric states. These results do not support a direct involvement of AtCCD4 in generating the supposed regulatory metabolites. In contrast, the strigolactone biosynthetic enzyme AtCCD7 converted 9-cis-configured acyclic carotenes, such as 9-cis-ζ-carotene, 9'-cis-neurosporene, and 9-cis-lycopene, yielding 9-cis-configured products and indicating that AtCCD7, rather than AtCCD4, is the candidate for forming acyclic retrograde signals. PMID:27811075

IOT Overview: Wide-Field Imaging

Science.gov (United States)

Selman, F. J.

The Wide Field Imager (WFI) instrument at La Silla has been the workhorse of wide-field imaging instruments at ESO for several years. In this contribution I will summarize the issues relating to its productivity for the community both in terms of the quality and quantity of data that has come out of it. Although only surveys of limited scope have been completed using WFI, it is ESO's stepping-stone to the new generation of survey telescopes.

Mosaic trisomy 8 detected by fibroblasts cultured of skin

Science.gov (United States)

Gómez, Ana M; Mora, Lina; Suarez-Obando, Fernando; Moreno, Olga

2016-01-01

Introduction: Mosaic trisomy 8 or "Warkany's Syndrome" is a chromosomopathy with an estimated prevalance of 1:25,000 to 1:50,000, whose clinical presentation has a wide phenotypic variability. Case Description: Patient aged 14 years old with antecedents of global retardation of development, moderate cognitive deficit and hypothyroidism of possible congenital origin. Clinical Findings: Physical examination revealed palpebral ptosis, small corneas and corectopia, hypoplasia of the upper maxilla and prognathism, dental crowding, high-arched palate, anomalies of the extremities such as digitalization of the thumbs, clinodactyly and bilateral shortening of the fifth finger, shortening of the right femur, columnar deviation and linear brown blotches that followed Blaschko's lines. Cerebral nuclear magnetic resonance revealed type 1 Chiari's malformation and ventriculomegaly. Although the karyotype was normal in peripheral blood (46,XY), based on the finding of cutaneous mosaicism the lesions were biopsied and cytogenetic analysis demonstrated mosaic trisomy 8: mos 47,XY,+8[7]/46,XY[93]. Clinical Relevance: Trisomy 8 is clinically presented as a mosaic, universal cases being unfailingly lethal. In this particular case, cutaneous lesions identified the mosaic in tissue, although the karyotype was normal in peripheral blood. The cutaneous mosaicism represented by brown linear blotches which follow Blaschko's lines is a clinical finding that has not previously been described in Warkany's syndrome. PMID:27546932

Flatfielding Errors in Strömvil CCD Photometry

Directory of Open Access Journals (Sweden)

Boyle R. P.

2003-12-01

Full Text Available The importance of determining the error of the flat field in CCD photometry is detailed and our methods of doing this are described. We now have reached a precision of 1-1.5 % in our photometry. Color-magnitude diagrams of the open cluster M67 (ours and Laugalys et al. 2003 are compared.

Correction of projective distortion in long-image-sequence mosaics without prior information

Science.gov (United States)

Yang, Chenhui; Mao, Hongwei; Abousleman, Glen; Si, Jennie

2010-04-01

Image mosaicking is the process of piecing together multiple video frames or still images from a moving camera to form a wide-area or panoramic view of the scene being imaged. Mosaics have widespread applications in many areas such as security surveillance, remote sensing, geographical exploration, agricultural field surveillance, virtual reality, digital video, and medical image analysis, among others. When mosaicking a large number of still images or video frames, the quality of the resulting mosaic is compromised by projective distortion. That is, during the mosaicking process, the image frames that are transformed and pasted to the mosaic become significantly scaled down and appear out of proportion with respect to the mosaic. As more frames continue to be transformed, important target information in the frames can be lost since the transformed frames become too small, which eventually leads to the inability to continue further. Some projective distortion correction techniques make use of prior information such as GPS information embedded within the image, or camera internal and external parameters. Alternatively, this paper proposes a new algorithm to reduce the projective distortion without using any prior information whatsoever. Based on the analysis of the projective distortion, we approximate the projective matrix that describes the transformation between image frames using an affine model. Using singular value decomposition, we can deduce the affine model scaling factor that is usually very close to 1. By resetting the image scale of the affine model to 1, the transformed image size remains unchanged. Even though the proposed correction introduces some error in the image matching, this error is typically acceptable and more importantly, the final mosaic preserves the original image size after transformation. We demonstrate the effectiveness of this new correction algorithm on two real-world unmanned air vehicle (UAV) sequences. The proposed method is

Wide-Field Imaging of Omega Centauri with the Advanced Camera for Surveys

Science.gov (United States)

Haggard, D.; Dorfman, J. L.; Cool, A. M.; Anderson, J.; Bailyn, C. D.; Edmonds, P. D.; Grindlay, J. E.

2003-12-01

We present initial results of a wide-field imaging study of the globular cluster Omega Cen (NGC 5139) using the Advanced Camera for Surveys (ACS). We have obtained a mosaic of 3x3 pointings of the cluster using the HST/ACS Wide Field Camera covering approximately 10' x 10', roughly out to the cluster's half-mass radius. Using F435W (B435), F625W (R625) and F658N (H-alpha) filters, we are searching for optical counterparts of Chandra X-ray sources and studying the cluster's stellar populations. Here we report the discovery of an optical counterpart to the X-ray source identified by Rutledge et al. (2002) as a possible quiescent neutron star on the basis of its X-ray spectrum. The star's magnitude and color (R625 = 24.4, B435-R625 = 1.5) place it more than 1.5 magnitudes to the blue side of the main sequence. Through the H-alpha filter it is about 1.3 magnitudes brighter than cluster stars of comparable R625 magnitude. The blue color and H-alpha excess suggest the presence of an accretion disk, implying that the neutron star is a member of a quiescent low-mass X-ray binary. The object's faint absolute magnitude (M625 ˜ 10.6, M435 ˜ 11.8) implies that the system contains an unusually weak disk and that the companion, if it is a main-sequence star, is of very low mass (ACS study. This work is supported by NASA grant GO-9442 from the Space Telescope Science Institute.

Charge diffusion in CCD X-ray detectors

International Nuclear Information System (INIS)

Pavlov, George G.; Nousek, John A.

1999-01-01

Critical to the detection of X-rays by CCDs, is the detailed process of charge diffusion and drift within the device. We reexamine the prescriptions currently used in the modeling of X-ray CCD detectors to provide analytic expressions for the charge distribution over the CCD pixels which are suitable for use in numerical simulations of CCD response. Our treatment results in models which predict charge distributions which are more centrally peaked and have flatter wings than the Gaussian shapes predicted by previous work and adopted in current CCD modeling codes

Fully depleted back-illuminated p-channel CCD development

Energy Technology Data Exchange (ETDEWEB)

Bebek, Chris J.; Bercovitz, John H.; Groom, Donald E.; Holland, Stephen E.; Kadel, Richard W.; Karcher, Armin; Kolbe, William F.; Oluseyi, Hakeem M.; Palaio, Nicholas P.; Prasad, Val; Turko, Bojan T.; Wang, Guobin

2003-07-08

An overview of CCD development efforts at Lawrence Berkeley National Laboratory is presented. Operation of fully-depleted, back-illuminated CCD's fabricated on high resistivity silicon is described, along with results on the use of such CCD's at ground-based observatories. Radiation damage and point-spread function measurements are described, as well as discussion of CCD fabrication technologies.

Tissue differences in fragile X mosaics: Mosaicism in blood cells may differ greatly from skin

Energy Technology Data Exchange (ETDEWEB)

Dobkin, C.S.; Nolin, S.L.; Cohen, I. [NYS Institute for Basic Research in Developmental Disabilities, Staten Island, NY (United States)] [and others

1996-08-09

The fragile X mutation is diagnosed from the structure of the FMR1 gene in blood cell DNA. An estimated 12 to 41% of affected males are mosaics who carry both a {open_quotes}full mutation{close_quotes} allele from which there is no gene expression and a {open_quotes}premutation{close_quotes} allele which has normal gene expression. We compared the DNA in blood cells and skin fibroblasts from four mosaic fragile X males to see if there was a difference in the relative amounts of premutation and full mutation alleles within the tissues of these individuals. Two of these males showed striking differences in the ratio of premutation to full mutation in different tissues while the other two showed only slight differences. These observations conform with the widely accepted hypothesis that the fragile X CGG repeat is unstable in somatic tissue during early embryogenesis. Accordingly, the mosaicism in brain and skin, which are both ectodermal in origin, may be similar to each other but different from blood which is not ectodermal in origin. Thus, the ratio of full mutation to premutation allele in skin fibroblasts might be a better indicator of psychological impairment than the ratio in blood cells. 24 refs., 4 figs., 1 tab.

Performance Evaluations for Super-Resolution Mosaicing on UAS Surveillance Videos

Directory of Open Access Journals (Sweden)

Aldo Camargo

2013-05-01

Full Text Available Abstract Unmanned Aircraft Systems (UAS have been widely applied for reconnaissance and surveillance by exploiting information collected from the digital imaging payload. The super-resolution (SR mosaicing of low-resolution (LR UAS surveillance video frames has become a critical requirement for UAS video processing and is important for further effective image understanding. In this paper we develop a novel super-resolution framework, which does not require the construction of sparse matrices. The proposed method implements image operations in the spatial domain and applies an iterated back-projection to construct super-resolution mosaics from the overlapping UAS surveillance video frames. The Steepest Descent method, the Conjugate Gradient method and the Levenberg-Marquardt algorithm are used to numerically solve the nonlinear optimization problem for estimating a super-resolution mosaic. A quantitative performance comparison in terms of computation time and visual quality of the super-resolution mosaics through the three numerical techniques is presented.

Counselling considerations for chromosomal mosaicism detected by preimplantation genetic screening.

Science.gov (United States)

Besser, Andria G; Mounts, Emily L

2017-04-01

The evolution of preimplantation genetic screening (PGS) for aneuploidy to blastocyst biopsy and more sensitive 24-chromosome screening techniques has resulted in a new diagnostic category of PGS results: those classified as mosaic. This diagnosis presents significant challenges for clinicians in developing policies regarding transfer and storage of such embryos, as well as in providing genetic counselling for patients prior to and following PGS. Given the high frequency of mosaic PGS results and the wide range of possible associated outcomes, there is an urgent need to understand how to appropriately counsel patients regarding such embryos. This is the first commentary to thoroughly address pre- and post-test genetic counselling recommendations, as well as considerations regarding prenatal screening and diagnosis. Current data on mosaic PGS results are summarized along with embryo selection considerations and potential outcomes of embryos diagnosed as mosaic. Copyright © 2017 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Female chromosome X mosaicism is age-related and preferentially affects the inactivated X chromosome

DEFF Research Database (Denmark)

Machiela, Mitchell J; Zhou, Weiyin; Karlins, Eric

2016-01-01

To investigate large structural clonal mosaicism of chromosome X, we analysed the SNP microarray intensity data of 38,303 women from cancer genome-wide association studies (20,878 cases and 17,425 controls) and detected 124 mosaic X events >2 Mb in 97 (0.25%) women. Here we show rates for X-chrom...

CCD's at TPC

International Nuclear Information System (INIS)

Zeller, M.E.

1977-01-01

The CCD, Charge Coupled Device, is an analog shift register for which application to the readout of particle detectors has recently been realized. These devices can be used to detect optical information directly, providing an automated readout for streamer or other optical chambers, or as a single input shift register, acting in this instance as a delay line for analog information. A description is given of the latter mode of operation and its utility as a readout method for drift chambers. Most of the information contained herein has been obtained from tests performed in connection with PEP TPC project, PEP-4. That detector will employ approximately 10 4 CCD's making it a reasonable testing ground for ISABELLE size detectors

Generation of High-Resolution Geo-referenced Photo-Mosaics From Navigation Data

Science.gov (United States)

Delaunoy, O.; Elibol, A.; Garcia, R.; Escartin, J.; Fornari, D.; Humphris, S.

2006-12-01

Optical images of the ocean floor are a rich source of data to understand biological and geological processes. However, due to the attenuation of light in sea water, the area covered by the optical systems is very reduced, and a large number of images are then needed in order to cover an area of interest, as individually they do not provide a global view of the surveyed area. Therefore, generating a composite view (or photo-mosaic) from multiple overlapping images is usually the most practical and flexible solution to visually cover a wide area, allowing the analysis of the site in one single representation of the ocean floor. In most of the camera surveys which are carried out nowadays, some sort of positioning information is available (e.g., USBL, DVL, INS, gyros, etc). If it is a towed camera an estimation of the length of the tether and the mother ship GPS reading could also serve as navigation data. In any case, a photo-mosaic can be build just by taking into account the position and orientation of the camera. On the other hand, most of the regions of interest for the scientific community are quite large (>1Km2) and since better resolution is always required, the final photo-mosaic can be very large (>1,000,000 × 1,000,000 pixels), and cannot be handled by commonly available software. For this reason, we have developed a software package able to load a navigation file and the sequence of acquired images to automatically build a geo-referenced mosaic. This navigated mosaic provides a global view of the interest site, at the maximum available resolution. The developed package includes a viewer, allowing the user to load, view and annotate these geo-referenced photo-mosaics on a personal computer. A software library has been developed to allow the viewer to manage such very big images. Therefore, the size of the resulting mosaic is now only limited by the size of the hard drive. Work is being carried out to apply image processing techniques to the navigated

In-flight characterization of the HETE soft X-ray CCD cameras

International Nuclear Information System (INIS)

Prigozhin, G.; Villasenor, J.; Vanderspek, R.; Doty, J.; Crew, G.; Ricker, G.; Jernigan, G.

2003-01-01

We have developed a set of software tools that allow to monitor the performance of the flight X-ray CCD cameras as soon as data arrive at MIT. An emission line at 5.9 keV from the on-board Fe-55 radioactive calibration source is clearly visible in the spectra and provides the means to measure the gain and the noise for each observation in each of the 4 CCD chips in operation. Both parameters can change with time, depending on the phase of the moon and the amount of light leaking into the system. Time vs. position scatter plots were found to be an extremely powerful tool in understanding of the device performance. They illustrate the evolution of the light leaks produced by the dark Earth at the beginning and the end of each orbit. With a bright X-ray source in the field of view the shadow of the mask projected on the surface of the CCD clearly shows the motions of the spacecraft

Development of a Data Reduction Algorithm for Optical Wide Field Patrol (OWL) II: Improving Measurement of Lengths of Detected Streaks

Science.gov (United States)

Park, Sun-Youp; Choi, Jin; Roh, Dong-Goo; Park, Maru; Jo, Jung Hyun; Yim, Hong-Suh; Park, Young-Sik; Bae, Young-Ho; Park, Jang-Hyun; Moon, Hong-Kyu; Choi, Young-Jun; Cho, Sungki; Choi, Eun-Jung

2016-09-01

As described in the previous paper (Park et al. 2013), the detector subsystem of optical wide-field patrol (OWL) provides many observational data points of a single artificial satellite or space debris in the form of small streaks, using a chopper system and a time tagger. The position and the corresponding time data are matched assuming that the length of a streak on the CCD frame is proportional to the time duration of the exposure during which the chopper blades do not obscure the CCD window. In the previous study, however, the length was measured using the diagonal of the rectangle of the image area containing the streak; the results were quite ambiguous and inaccurate, allowing possible matching error of positions and time data. Furthermore, because only one (position, time) data point is created from one streak, the efficiency of the observation decreases. To define the length of a streak correctly, it is important to locate the endpoints of a streak. In this paper, a method using a differential convolution mask pattern is tested. This method can be used to obtain the positions where the pixel values are changed sharply. These endpoints can be regarded as directly detected positional data, and the number of data points is doubled by this result.

CLEMENTINE HIRES MOSAIC

Data.gov (United States)

National Aeronautics and Space Administration — This CD contains portions of the Clementine HiRes Lunar Mosaic, a geometrically controlled, calibrated mosaic compiled from non-uniformity corrected, 750 nanometer...

Purification and crystallization of Vibrio fischeri CcdB and its complexes with fragments of gyrase and CcdA

International Nuclear Information System (INIS)

De Jonge, Natalie; Buts, Lieven; Vangelooven, Joris; Mine, Natacha; Van Melderen, Laurence; Wyns, Lode; Loris, Remy

2007-01-01

A CcdB homologue from V. fischeri was overexpressed in E. coli and purified. The free protein was crystallized, as were its complexes with fragments of E. coli and V. fischeri gyrase and with the F-plasmid CcdA C-terminal domain. The ccd toxin–antitoxin module from the Escherichia coli F plasmid has a homologue on the Vibrio fischeri integron. The homologue of the toxin (CcdB Vfi ) was crystallized in two different crystal forms. The first form belongs to space group I23 or I2 1 3, with unit-cell parameter a = 84.5 Å, and diffracts to 1.5 Å resolution. The second crystal form belongs to space group C2, with unit-cell parameters a = 58.5, b = 43.6, c = 37.5 Å, β = 110.0°, and diffracts to 1.7 Å resolution. The complex of CcdB Vfi with the GyrA14 Vfi fragment of V. fischeri gyrase crystallizes in space group P2 1 2 1 2 1 , with unit-cell parameters a = 53.5, b = 94.6, c = 58.1 Å, and diffracts to 2.2 Å resolution. The corresponding mixed complex with E. coli GyrA14 Ec crystallizes in space group C2, with unit-cell parameters a = 130.1, b = 90.8, c = 58.1 Å, β = 102.6°, and diffracts to 1.95 Å. Finally, a complex between CcdB Vfi and part of the F-plasmid antitoxin CcdA F crystallizes in space group P2 1 2 1 2 1 , with unit-cell parameters a = 46.9, b = 62.6, c = 82.0 Å, and diffracts to 1.9 Å resolution

STIS-01 CCD Functional

Science.gov (United States)

Valenti, Jeff

2001-07-01

This activity measures the baseline performance and commandability of the CCD subsystem. Only primary amplifier D is used. Bias, Dark, and Flat Field exposures are taken in order to measure read noise, dark current, CTE, and gain. Numerous bias frames are taken to permit construction of "superbias" frames in which the effects of read noise have been rendered negligible. Dark exposures are made outside the SAA. Full frame and binned observations are made, with binning factors of 1x1 and 2x2. Finally, tungsten lamp exposures are taken through narrow slits to confirm the slit positions in the current database. All exposures are internals. This is a reincarnation of SM3A proposal 8502 with some unnecessary tests removed from the program.

Recording of radiation-induced optical density changes in doped agarose gels with a CCD camera

International Nuclear Information System (INIS)

Tarte, B.J.; Jardine, P.A.; Van Doorn, T.

1996-01-01

Full text: Spatially resolved dose measurement with iron-doped agarose gels is continuing to be investigated for applications in radiotherapy dosimetry. It has previously been proposed to use optical methods, rather than MRI, for dose measurement with such gels and this has been investigated using a spectrophotometer (Appleby A and Leghrouz A, Med Phys, 18:309-312, 1991). We have previously studied the use of a pencil beam laser for such optical density measurement of gels and are currently investigating charge-coupled devices (CCD) camera imaging for the same purpose but with the advantages of higher data acquisition rates and potentially greater spatial resolution. The gels used in these studies were poured, irradiated and optically analysed in Perspex casts providing gel sections 1 cm thick and up to 20 cm x 30 cm in dimension. The gels were also infused with a metal indicator dye (xylenol orange) to render the radiation induced oxidation of the iron in the gel sensitive to optical radiation, specifically in the green spectral region. Data acquisition with the CCD camera involved illumination of the irradiated gel section with a diffuse white light source, with the light from the plane of the gel section focussed to the CCD array with a manual zoom lens. The light was also filtered with a green colour glass filter to maximise the contrast between unirradiated and irradiated gels. The CCD camera (EG and G Reticon MC4013) featured a 1024 x 1024 pixel array and was interfaced to a PC via a frame grabber acquisition board with 8 bit resolution. The performance of the gel dosimeter was appraised in mapping of physical and dynamic wedged 6 MV X-ray fields. The results from the CCD camera detection system were compared with both ionisation chamber data and laser based optical density measurements of the gels. Cross beam profiles were extracted from each measurement system at a particular depth (eg. 2.3 cm for the physical wedge field) for direct comparison. A

Follow-up study of children with cerebral coordination disturbance (CCD, Vojta).

Science.gov (United States)

Imamura, S; Sakuma, K; Takahashi, T

1983-01-01

713 children (from newborn to 12-month-old) with delayed motor development were carefully examined and classified into normal, very light cerebral coordination disturbance (CCD, Vojta), light CCD, moderate CCD, severe CCD, suspected cerebral palsy (CP) and other diseases at their first visit, and were followed up carefully. Finally, 89.0% of very light CCD, 71.4% of light CCD, 56.0% of moderate CCD and 30.0% of severe CCD developed into normal. 59.5% of moderate CCD and 45.5% of severe CCD among children who were given Vojta's physiotherapy developed into normal. The classification of cases with delayed motor development into very light, light, moderate and severe CCD based on the extent of abnormality in their postural reflexes is useful and well correlated with their prognosis. Treatment by Vojta's method seems to be efficient and helpful for young children with delayed motor development.

THEMIS Global Mosaics

Science.gov (United States)

Gorelick, N. S.; Christensen, P. R.

2005-12-01

We have developed techniques to make seamless, controlled global mosaics from the more than 50,000 multi-spectral infrared images of the Mars returned by the THEMIS instrument aboard the Mars Odyssey spacecraft. These images cover more than 95% of the surface at 100m/pixel resolution at both day and night local times. Uncertainties in the position and pointing of the spacecraft, varying local time, and imaging artifacts make creating well-registered mosaics from these datasets a challenging task. In preparation for making global mosaics, many full-resolution regional mosaics have been made. These mosaics typically cover an area 10x10 degrees or smaller, and are constructed from only a few hundred images. To make regional mosaics, individual images are geo-rectified using the USGS ISIS software. This dead-reckoning is sufficient to approximate position to within 400m in cases where the SPICE information was downlinked. Further coregistration of images is handled in two ways: grayscale differences minimization in overlapping regions through integer pixel shifting, or through automatic tie-point generation using a radial symmetry transformation (RST). The RST identifies points within an image that exhibit 4-way symmetry. Martian craters tend to to be very radially symmetric, and the RST can pin-point a crater center to sub-pixel accuracy in both daytime and nighttime images, independent of lighting, time of day, or seasonal effects. Additionally, the RST works well on visible-light images, and in a 1D application, on MOLA tracks, to provide precision tie-points across multiple data sets. The RST often finds many points of symmetry that aren't related to surface features. These "false-hits" are managed using a clustering algorithm that identifies constellations of points that occur in multiple images, independent of scaling or other affine transformations. This technique is able to make use of data in which the "good" tie-points comprise even less than 1% of total

Purification and crystallization of Vibrio fischeri CcdB and its complexes with fragments of gyrase and CcdA

Energy Technology Data Exchange (ETDEWEB)

De Jonge, Natalie, E-mail: ndejonge@vub.ac.be; Buts, Lieven; Vangelooven, Joris [Department of Molecular and Cellular Interactions, VIB, Pleinlaan 2, 1050 Brussels (Belgium); Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels (Belgium); Mine, Natacha; Van Melderen, Laurence [Laboratoire de Génétique des Procaryotes, Institut de Biologie et de Médecine, Université Libre de Bruxelles, Gosselies (Belgium); Wyns, Lode; Loris, Remy [Department of Molecular and Cellular Interactions, VIB, Pleinlaan 2, 1050 Brussels (Belgium); Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels (Belgium)

2007-04-01

A CcdB homologue from V. fischeri was overexpressed in E. coli and purified. The free protein was crystallized, as were its complexes with fragments of E. coli and V. fischeri gyrase and with the F-plasmid CcdA C-terminal domain. The ccd toxin–antitoxin module from the Escherichia coli F plasmid has a homologue on the Vibrio fischeri integron. The homologue of the toxin (CcdB{sub Vfi}) was crystallized in two different crystal forms. The first form belongs to space group I23 or I2{sub 1}3, with unit-cell parameter a = 84.5 Å, and diffracts to 1.5 Å resolution. The second crystal form belongs to space group C2, with unit-cell parameters a = 58.5, b = 43.6, c = 37.5 Å, β = 110.0°, and diffracts to 1.7 Å resolution. The complex of CcdB{sub Vfi} with the GyrA14{sub Vfi} fragment of V. fischeri gyrase crystallizes in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 53.5, b = 94.6, c = 58.1 Å, and diffracts to 2.2 Å resolution. The corresponding mixed complex with E. coli GyrA14{sub Ec} crystallizes in space group C2, with unit-cell parameters a = 130.1, b = 90.8, c = 58.1 Å, β = 102.6°, and diffracts to 1.95 Å. Finally, a complex between CcdB{sub Vfi} and part of the F-plasmid antitoxin CcdA{sub F} crystallizes in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 46.9, b = 62.6, c = 82.0 Å, and diffracts to 1.9 Å resolution.

Noise characteristics of neutron images obtained by cooled CCD device

International Nuclear Information System (INIS)

Taniguchi, Ryoichi; Sasaki, Ryoya; Okuda, Shuichi; Okamoto, Ken-Ichi; Ogawa, Yoshihiro; Tsujimoto, Tadashi

2009-01-01

The noise characteristics of a cooled CCD device induced by neutron and gamma ray irradiation have been investigated. In the cooled CCD images, characteristic white spot noises (CCD noise) frequently appeared, which have a shape like a pixel in most cases and their brightness is extremely high compared with that of the image pattern. They could be divided into the two groups, fixed pattern noise (FPN) and random noise. The former always appeared in the same position in the image and the latter appeared at any position. In the background image, nearly all of the CCD noises were found to be the FPN, while many of them were the random noise during the irradiation. The random CCD noises increased with irradiation and decreased soon after the irradiation. In the case of large irradiation, a part of the CCD noise remained as the FPN. These facts suggest that the CCD noise is a phenomenon strongly relating to radiation damage of the CCD device.

Ultra-wide-field imaging in diabetic retinopathy.

Science.gov (United States)

Ghasemi Falavarjani, Khalil; Tsui, Irena; Sadda, Srinivas R

2017-10-01

Since 1991, 7-field images captured with 30-50 degree cameras in the Early Treatment Diabetic Retinopathy Study were the gold standard for fundus imaging to study diabetic retinopathy. Ultra-wide-field images cover significantly more area (up to 82%) of the fundus and with ocular steering can in many cases image 100% of the fundus ("panretinal"). Recent advances in image analysis of ultra-wide-field imaging allow for precise measurements of the peripheral retinal lesions. There is a growing consensus in the literature that ultra-wide-field imaging improves detection of peripheral lesions in diabetic retinopathy and leads to more accurate classification of the disease. There is discordance among studies, however, on the correlation between peripheral diabetic lesions and diabetic macular edema and optimal management strategies to treat diabetic retinopathy. Copyright © 2017 Elsevier Ltd. All rights reserved.

Characterization of Large Structural Genetic Mosaicism in Human Autosomes

Science.gov (United States)

Machiela, Mitchell J.; Zhou, Weiyin; Sampson, Joshua N.; Dean, Michael C.; Jacobs, Kevin B.; Black, Amanda; Brinton, Louise A.; Chang, I-Shou; Chen, Chu; Chen, Constance; Chen, Kexin; Cook, Linda S.; Crous Bou, Marta; De Vivo, Immaculata; Doherty, Jennifer; Friedenreich, Christine M.; Gaudet, Mia M.; Haiman, Christopher A.; Hankinson, Susan E.; Hartge, Patricia; Henderson, Brian E.; Hong, Yun-Chul; Hosgood, H. Dean; Hsiung, Chao A.; Hu, Wei; Hunter, David J.; Jessop, Lea; Kim, Hee Nam; Kim, Yeul Hong; Kim, Young Tae; Klein, Robert; Kraft, Peter; Lan, Qing; Lin, Dongxin; Liu, Jianjun; Le Marchand, Loic; Liang, Xiaolin; Lissowska, Jolanta; Lu, Lingeng; Magliocco, Anthony M.; Matsuo, Keitaro; Olson, Sara H.; Orlow, Irene; Park, Jae Yong; Pooler, Loreall; Prescott, Jennifer; Rastogi, Radhai; Risch, Harvey A.; Schumacher, Fredrick; Seow, Adeline; Setiawan, Veronica Wendy; Shen, Hongbing; Sheng, Xin; Shin, Min-Ho; Shu, Xiao-Ou; VanDen Berg, David; Wang, Jiu-Cun; Wentzensen, Nicolas; Wong, Maria Pik; Wu, Chen; Wu, Tangchun; Wu, Yi-Long; Xia, Lucy; Yang, Hannah P.; Yang, Pan-Chyr; Zheng, Wei; Zhou, Baosen; Abnet, Christian C.; Albanes, Demetrius; Aldrich, Melinda C.; Amos, Christopher; Amundadottir, Laufey T.; Berndt, Sonja I.; Blot, William J.; Bock, Cathryn H.; Bracci, Paige M.; Burdett, Laurie; Buring, Julie E.; Butler, Mary A.; Carreón, Tania; Chatterjee, Nilanjan; Chung, Charles C.; Cook, Michael B.; Cullen, Michael; Davis, Faith G.; Ding, Ti; Duell, Eric J.; Epstein, Caroline G.; Fan, Jin-Hu; Figueroa, Jonine D.; Fraumeni, Joseph F.; Freedman, Neal D.; Fuchs, Charles S.; Gao, Yu-Tang; Gapstur, Susan M.; Patiño-Garcia, Ana; Garcia-Closas, Montserrat; Gaziano, J. Michael; Giles, Graham G.; Gillanders, Elizabeth M.; Giovannucci, Edward L.; Goldin, Lynn; Goldstein, Alisa M.; Greene, Mark H.; Hallmans, Goran; Harris, Curtis C.; Henriksson, Roger; Holly, Elizabeth A.; Hoover, Robert N.; Hu, Nan; Hutchinson, Amy; Jenab, Mazda; Johansen, Christoffer; Khaw, Kay-Tee; Koh, Woon-Puay; Kolonel, Laurence N.; Kooperberg, Charles; Krogh, Vittorio; Kurtz, Robert C.; LaCroix, Andrea; Landgren, Annelie; Landi, Maria Teresa; Li, Donghui; Liao, Linda M.; Malats, Nuria; McGlynn, Katherine A.; McNeill, Lorna H.; McWilliams, Robert R.; Melin, Beatrice S.; Mirabello, Lisa; Peplonska, Beata; Peters, Ulrike; Petersen, Gloria M.; Prokunina-Olsson, Ludmila; Purdue, Mark; Qiao, You-Lin; Rabe, Kari G.; Rajaraman, Preetha; Real, Francisco X.; Riboli, Elio; Rodríguez-Santiago, Benjamín; Rothman, Nathaniel; Ruder, Avima M.; Savage, Sharon A.; Schwartz, Ann G.; Schwartz, Kendra L.; Sesso, Howard D.; Severi, Gianluca; Silverman, Debra T.; Spitz, Margaret R.; Stevens, Victoria L.; Stolzenberg-Solomon, Rachael; Stram, Daniel; Tang, Ze-Zhong; Taylor, Philip R.; Teras, Lauren R.; Tobias, Geoffrey S.; Viswanathan, Kala; Wacholder, Sholom; Wang, Zhaoming; Weinstein, Stephanie J.; Wheeler, William; White, Emily; Wiencke, John K.; Wolpin, Brian M.; Wu, Xifeng; Wunder, Jay S.; Yu, Kai; Zanetti, Krista A.; Zeleniuch-Jacquotte, Anne; Ziegler, Regina G.; de Andrade, Mariza; Barnes, Kathleen C.; Beaty, Terri H.; Bierut, Laura J.; Desch, Karl C.; Doheny, Kimberly F.; Feenstra, Bjarke; Ginsburg, David; Heit, John A.; Kang, Jae H.; Laurie, Cecilia A.; Li, Jun Z.; Lowe, William L.; Marazita, Mary L.; Melbye, Mads; Mirel, Daniel B.; Murray, Jeffrey C.; Nelson, Sarah C.; Pasquale, Louis R.; Rice, Kenneth; Wiggs, Janey L.; Wise, Anastasia; Tucker, Margaret; Pérez-Jurado, Luis A.; Laurie, Cathy C.; Caporaso, Neil E.; Yeager, Meredith; Chanock, Stephen J.

2015-01-01

Analyses of genome-wide association study (GWAS) data have revealed that detectable genetic mosaicism involving large (>2 Mb) structural autosomal alterations occurs in a fraction of individuals. We present results for a set of 24,849 genotyped individuals (total GWAS set II [TGSII]) in whom 341 large autosomal abnormalities were observed in 168 (0.68%) individuals. Merging data from the new TGSII set with data from two prior reports (the Gene-Environment Association Studies and the total GWAS set I) generated a large dataset of 127,179 individuals; we then conducted a meta-analysis to investigate the patterns of detectable autosomal mosaicism (n = 1,315 events in 925 [0.73%] individuals). Restricting to events >2 Mb in size, we observed an increase in event frequency as event size decreased. The combined results underscore that the rate of detectable mosaicism increases with age (p value = 5.5 × 10−31) and is higher in men (p value = 0.002) but lower in participants of African ancestry (p value = 0.003). In a subset of 47 individuals from whom serial samples were collected up to 6 years apart, complex changes were noted over time and showed an overall increase in the proportion of mosaic cells as age increased. Our large combined sample allowed for a unique ability to characterize detectable genetic mosaicism involving large structural events and strengthens the emerging evidence of non-random erosion of the genome in the aging population. PMID:25748358

Comparative Analysis of Chinese HJ-1 CCD, GF-1 WFV and ZY-3 MUX Sensor Data for Leaf Area Index Estimations for Maize

Directory of Open Access Journals (Sweden)

Jing Zhao

2018-01-01

Full Text Available In recent years, China has developed and launched several satellites with high spatial resolutions, such as the resources satellite No. 3 (ZY-3 with a multi-spectral camera (MUX and 5.8 m spatial resolution, the satellite GaoFen No. 1 (GF-1 with a wide field of view (WFV camera and 16 m spatial resolution, and the environment satellite (HJ-1A/B with a charge-coupled device (CCD sensor and 30 m spatial resolution. First, to analyze the potential application of ZY-3 MUX, GF-1 WFV, and HJ-1 CCD to extract the leaf area index (LAI at the regional scale, this study estimated LAI from the relationships between physical model-based spectral vegetation indices (SVIs and LAI values that were generated from look-up tables (LUTs, simulated from the combination of the PROSPECT-5B leaf model and the scattering by arbitrarily inclined leaves with the hot-spot effect (SAILH canopy reflectance model. Second, to assess the surface reflectance quality of these sensors after data preprocessing, the well-processed surface reflectance products of the Landsat-8 operational land imager (OLI sensor with a convincing data quality were used to compare the performances of ZY-3 MUX, GF-1 WFV, and HJ-1 CCD sensors both in theory and reality. Apart from several reflectance fluctuations, the reflectance trends were coincident, and the reflectance values of the red and near-infrared (NIR bands were comparable among these sensors. Finally, to analyze the accuracy of the LAI estimated from ZY-3 MUX, GF-1 WFV, and HJ-1 CCD, the LAI estimations from these sensors were validated based on LAI field measurements in Huailai, Hebei Province, China. The results showed that the performance of the LAI that was inversed from ZY-3 MUX was better than that from GF-1 WFV, and HJ-1 CCD, both of which tended to be systematically underestimated. In addition, the value ranges and accuracies of the LAI inversions both decreased with decreasing spatial resolution.

Flat Field Anomalies in an X-ray CCD Camera Measured Using a Manson X-ray Source (HTPD 08 paper)

International Nuclear Information System (INIS)

Haugh, M; Schneider, M B

2008-01-01

The Static X-ray Imager (SXI) is a diagnostic used at the National Ignition Facility (NIF) to measure the position of the X-rays produced by lasers hitting a gold foil target. The intensity distribution taken by the SXI camera during a NIF shot is used to determine how accurately NIF can aim laser beams. This is critical to proper NIF operation. Imagers are located at the top and the bottom of the NIF target chamber. The CCD chip is an X-ray sensitive silicon sensor, with a large format array (2k x 2k), 24 (micro)m square pixels, and 15 (micro)m thick. A multi-anode Manson X-ray source, operating up to 10kV and 10W, was used to characterize and calibrate the imagers. The output beam is heavily filtered to narrow the spectral beam width, giving a typical resolution E/ΔE ∼ 10. The X-ray beam intensity was measured using an absolute photodiode that has accuracy better than 1% up to the Si K edge and better than 5% at higher energies. The X-ray beam provides full CCD illumination and is flat, within ±1% maximum to minimum. The spectral efficiency was measured at 10 energy bands ranging from 930 eV to 8470 eV. We observed an energy dependent pixel sensitivity variation that showed continuous change over a large portion of the CCD. The maximum sensitivity variation occurred at 8470 eV. The geometric pattern did not change at lower energies, but the maximum contrast decreased and was not observable below 4 keV. We were also able to observe debris, damage, and surface defects on the CCD chip. The Manson source is a powerful tool for characterizing the imaging errors of an X-ray CCD imager. These errors are quite different from those found in a visible CCD imager

Retrieval of forest biomass for tropical deciduous mixed forest using ALOS PALSAR mosaic imagery and field plot data

Science.gov (United States)

Ningthoujam, Ramesh K.; Joshi, P. K.; Roy, P. S.

2018-07-01

Tropical forest is an important ecosystem rich in biodiversity and structural complexity with high woody biomass content. Longer wavelength radar data at L-band sensor provides improved forest biomass (AGB) information due to its higher penetration level and sensitivity to canopy structure. The study presents a regression based woody biomass estimation for tropical deciduous mixed forest dominated by Shorea robusta using ALOS PALSAR mosaic (HH, HV) and field data at the lower Himalayan belt of Northern India. For the purpose of understanding the scattering mechanisms at L-band from this forest type, Michigan Microwave Canopy Scattering model (MIMICS-I) was parameterized with field data to simulate backscatter across polarization and incidence range. Regression analysis between field measured forest biomass and L-band backscatter data from PALSAR mosaic show retrieval of woody biomass up to 100 Mg ha-1 with error between 92 and 94 Mg ha-1 and coefficient of determination (r2) between 0.53 and 0.55 for HH and HH + HV polarized channel at 0.25 ha resolution. This positive relationship could be due to strong volume scattering from ground/trunk interaction at HH-polarized while in combination with direct canopy scattering for HV-polarization at ALOS specific incidence angles as predicted by MIMICS-I model. This study has found that L-band SAR data from currently ALOS-1/-2 and upcoming joint NASA-ISRO SAR (NISAR) are suitable for mapping forest biomass ≤100 Mg ha-1 at 25 m resolution in far incidence range in dense deciduous mixed forest of Northern India.

IMAGE-2006 Mosaic: Product Description

OpenAIRE

SOILLE Pierre; BIELSKI Conrad

2008-01-01

This report describes the IMAGE-2006 mosaic products. Each product consists of a range of information layers grouped into three categories: base layers, mosaic layers, and quality layers. A mosaic product is available for each coverage and data/country region of interest combination.

CCD-based vertex detectors

CERN Document Server

Damerell, C J S

2005-01-01

Over the past 20 years, CCD-based vertex detectors have been used to construct some of the most precise 'tracking microscopes' in particle physics. They were initially used by the ACCMOR collaboration for fixed target experiments in CERN, where they enabled the lifetimes of some of the shortest-lived charm particles to be measured precisely. The migration to collider experiments was accomplished in the SLD experiment, where the original 120 Mpixel detector was later upgraded to one with 307 Mpixels. This detector was used in a range of physics studies which exceeded the capability of the LEP detectors, including the most precise limit to date on the Bs mixing parameter. This success, and the high background hit densities that will inevitably be encountered at the future TeV-scale linear collider, have established the need for a silicon pixel-based vertex detector at this machine. The technical options have now been broadened to include a wide range of possible silicon imaging technologies as well as CCDs (mon...

CCD Astrophotography High-Quality Imaging from the Suburbs

CERN Document Server

Stuart, Adam

2006-01-01

This is a reference book for amateur astronomers who have become interested in CCD imaging. Those glorious astronomical images found in astronomy magazines might seem out of reach to newcomers to CCD imaging, but this is not the case. Great pictures are attainable with modest equipment. Adam Stuart’s many beautiful images, reproduced in this book, attest to the quality of – initially – a beginner’s efforts. Chilled-chip astronomical CCD-cameras and software are also wonderful tools for cutting through seemingly impenetrable light-pollution. CCD Astrophotography from the Suburbs describes one man’s successful approach to the problem of getting high-quality astronomical images under some of the most light-polluted conditions. Here is a complete and thoroughly tested program that will help every CCD-beginner to work towards digital imaging of the highest quality. It is equally useful to astronomers who have perfect observing conditions, as to those who have to observe from light-polluted city skies.

Mosaicism for dominant collagen 6 mutations as a cause for intrafamilial phenotypic variability.

Science.gov (United States)

Donkervoort, Sandra; Hu, Ying; Stojkovic, Tanya; Voermans, Nicol C; Foley, A Reghan; Leach, Meganne E; Dastgir, Jahannaz; Bolduc, Véronique; Cullup, Thomas; de Becdelièvre, Alix; Yang, Lin; Su, Hai; Meilleur, Katherine; Schindler, Alice B; Kamsteeg, Erik-Jan; Richard, Pascale; Butterfield, Russell J; Winder, Thomas L; Crawford, Thomas O; Weiss, Robert B; Muntoni, Francesco; Allamand, Valérie; Bönnemann, Carsten G

2015-01-01

Collagen 6-related dystrophies and myopathies (COL6-RD) are a group of disorders that form a wide phenotypic spectrum, ranging from severe Ullrich congenital muscular dystrophy, intermediate phenotypes, to the milder Bethlem myopathy. Both inter- and intrafamilial variable expressivity are commonly observed. We present clinical, immunohistochemical, and genetic data on four COL6-RD families with marked intergenerational phenotypic heterogeneity. This variable expression seemingly masquerades as anticipation is due to parental mosaicism for a dominant mutation, with subsequent full inheritance and penetrance of the mutation in the heterozygous offspring. We also present an additional fifth simplex patient identified as a mosaic carrier. Parental mosaicism was confirmed in the four families through quantitative analysis of the ratio of mutant versus wild-type allele (COL6A1, COL6A2, and COL6A3) in genomic DNA from various tissues, including blood, dermal fibroblasts, and saliva. Consistent with somatic mosaicism, parental samples had lower ratios of mutant versus wild-type allele compared with the fully heterozygote offspring. However, there was notable variability of the mutant allele levels between tissues tested, ranging from 16% (saliva) to 43% (fibroblasts) in one mosaic father. This is the first report demonstrating mosaicism as a cause of intrafamilial/intergenerational variability of COL6-RD, and suggests that sporadic and parental mosaicism may be more common than previously suspected. © 2014 WILEY PERIODICALS, INC.

X-ray diffraction from ideal mosaic crystals in external fields of certain types. I. Atomic displacements and the corresponding diffraction patterns

International Nuclear Information System (INIS)

Treushnikov, E.N.

2000-01-01

The problem of the theoretical description of X-ray diffraction from ideal mosaic crystals under the effect of various external fields has been formulated. Electric, magnetic, electromagnetic, and acoustic perturbations are considered. The atomic displacements in crystals under the effect of external fields and the types of the corresponding diffraction patterns are analyzed for various types of perturbations. The crystal classes are determined in which atomic displacements can be recorded experimentally. Diffraction patterns formed under the effect of various external factors are considered on the basis of the derived dependence of the structure factor on the characteristics of an applied force field

A full year of snow on sea ice observations and simulations - Plans for MOSAiC 2019/20

Science.gov (United States)

Nicolaus, M.; Geland, S.; Perovich, D. K.

2017-12-01

The snow cover on sea on sea ice dominates many exchange processes and properties of the ice covered polar oceans. It is a major interface between the atmosphere and the sea ice with the ocean underneath. Snow on sea ice is known for its extraordinarily large spatial and temporal variability from micro scales and minutes to basin wide scales and decades. At the same time, snow cover properties and even snow depth distributions are among the least known and most difficult to observe climate variables. Starting in October 2019 and ending in October 2020, the international MOSAiC drift experiment will allow to observe the evolution of a snow pack on Arctic sea ice over a full annual cycle. During the drift with one ice floe along the transpolar drift, we will study snow processes and interactions as one of the main topics of the MOSAiC research program. Thus we will, for the first time, be able to perform such studies on seasonal sea ice and relate it to previous expeditions and parallel observations at different locations. Here we will present the current status of our planning of the MOSAiC snow program. We will summarize the latest implementation ideas to combine the field observations with numerical simulations. The field program will include regular manual observations and sampling on the main floe of the central observatory, autonomous recordings in the distributed network, airborne observations in the surrounding of the central observatory, and retrievals of satellite remote sensing products. Along with the field program, numerical simulations of the MOSAiC snow cover will be performed on different scales, including large-scale interaction with the atmosphere and the sea ice. The snow studies will also bridge between the different disciplines, including physical, chemical, biological, and geochemical measurements, samples, and fluxes. The main challenge of all measurements will be to accomplish the description of the full annual cycle.

Custom CCD for adaptive optics applications

Science.gov (United States)

Downing, Mark; Arsenault, Robin; Baade, Dietrich; Balard, Philippe; Bell, Ray; Burt, David; Denney, Sandy; Feautrier, Philippe; Fusco, Thierry; Gach, Jean-Luc; Diaz Garcia, José Javier; Guillaume, Christian; Hubin, Norbert; Jorden, Paul; Kasper, Markus; Meyer, Manfred; Pool, Peter; Reyes, Javier; Skegg, Michael; Stadler, Eric; Suske, Wolfgang; Wheeler, Patrick

2006-06-01

ESO and JRA2 OPTICON have funded e2v technologies to develop a compact packaged Peltier cooled 24 μm square 240x240 pixels split frame transfer 8-output back-illuminated L3Vision CCD3, L3Vision CCD for Adaptive Optic Wave Front Sensor (AO WFS) applications. The device is designed to achieve sub-electron read noise at frame rates from 25 Hz to 1,500 Hz and dark current lower than 0.01 e-/pixel/frame. The development has many unique features. To obtain high frame rates, multi-output EMCCD gain registers and metal buttressing of row clock lines are used. The baseline device is built in standard silicon. In addition, a split wafer run has enabled two speculative variants to be built; deep depletion silicon devices to improve red response and devices with an electronic shutter to extend use to Rayleigh and Pulsed Laser Guide Star applications. These are all firsts for L3Vision CCDs. The designs of the CCD and Peltier package have passed their reviews and fabrication has begun. This paper will describe the progress to date, the requirements and the design of the CCD and compact Peltier package, technology trade-offs, schedule and proposed test plan. High readout speed, low noise and compactness (requirement to fit in confined spaces) provide special challenges to ESO's AO variant of its NGC, New General detector Controller to drive this CCD. This paper will describe progress made on the design of the controller to meet these special needs.

Infantile spasms and pigmentary mosaicism

DEFF Research Database (Denmark)

Hansen, Lars K; Bygum, Anette; Krogh, Lotte N

2010-01-01

Summary We present a 3-year-old boy with pigmentary mosaicism and persistent intractable infantile spasms due to mosaicism of chromosome 7. Getting the diagnosis of pigmentary mosaicism in a child with infantile spasms may not be easy, as most diagnostic work-up is done in infancy, at a time when...

Correlation and image compression for limited-bandwidth CCD.

Energy Technology Data Exchange (ETDEWEB)

Thompson, Douglas G.

2005-07-01

As radars move to Unmanned Aerial Vehicles with limited-bandwidth data downlinks, the amount of data stored and transmitted with each image becomes more significant. This document gives the results of a study to determine the effect of lossy compression in the image magnitude and phase on Coherent Change Detection (CCD). We examine 44 lossy compression types, plus lossless zlib compression, and test each compression method with over 600 CCD image pairs. We also derive theoretical predictions for the correlation for most of these compression schemes, which compare favorably with the experimental results. We recommend image transmission formats for limited-bandwidth programs having various requirements for CCD, including programs which cannot allow performance degradation and those which have stricter bandwidth requirements at the expense of CCD performance.

3D Photo Mosaicing of Tagiri Shallow Vent Field by an Autonomous Underwater Vehicle

Science.gov (United States)

Maki, Toshihiro; Kondo, Hayato; Ura, Tamaki; Sakamaki, Takashi; Mizushima, Hayato; Yanagisawa, Masao

Although underwater visual observation is an ideal method for detailed survey of seafloors, it is currently a costly process that requires the use of Remotely Operated Vehicles (ROVs) or Human Occupied Vehicles (HOVs), and can cover only a limited area. This paper proposes an innovative method to navigate an autonomous underwater vehicle (AUV) to create both 2D and 3D photo mosaics of seafloors with high positioning accuracy without using any vision-based matching. The vehicle finds vertical pole-like acoustic reflectors to use as positioning landmarks using a profiling sonar based on a SLAM (Simultaneous Localization And Mapping) technique. These reflectors can be either artificial or natural objects, and so the method can be applied to shallow vent fields where conventional acoustic positioning is difficult, since bubble plumes can also be used as landmarks as well as artificial reflectors. Path-planning is performed in real-time based on the positions and types of landmarks so as to navigate safely and stably using landmarks of different types (artificial reflector or bubble plume) found at arbitrary times and locations. Terrain tracker switches control reference between depth and altitude from the seafloor based on a local map of hazardous area created in real-time using onboard perceptual sensors, in order to follow rugged terrains at an altitude of 1 to 2 meters, as this range is ideal for visual observation. The method was implemented in the AUV Tri-Dog 1 and experiments were carried out at Tagiri vent field, Kagoshima Bay in Japan. The AUV succeeded in fully autonomous observation for more than 160 minutes to create a photo mosaic with an area larger than 600 square meters, which revealed the spatial distribution of detailed features such as tube-worm colonies, bubble plumes and bacteria mats. A fine bathymetry of the same area was also created using a light-section ranging system mounted on the vehicle. Finally a 3 D representation of the environment was

Typical effects of laser dazzling CCD camera

Science.gov (United States)

Zhang, Zhen; Zhang, Jianmin; Shao, Bibo; Cheng, Deyan; Ye, Xisheng; Feng, Guobin

2015-05-01

In this article, an overview of laser dazzling effect to buried channel CCD camera is given. The CCDs are sorted into staring and scanning types. The former includes the frame transfer and interline transfer types. The latter includes linear and time delay integration types. All CCDs must perform four primary tasks in generating an image, which are called charge generation, charge collection, charge transfer and charge measurement. In camera, the lenses are needed to input the optical signal to the CCD sensors, in which the techniques for erasing stray light are used. And the electron circuits are needed to process the output signal of CCD, in which many electronic techniques are used. The dazzling effects are the conjunct result of light distribution distortion and charge distribution distortion, which respectively derive from the lens and the sensor. Strictly speaking, in lens, the light distribution is not distorted. In general, the lens are so well designed and fabricated that its stray light can be neglected. But the laser is of much enough intensity to make its stray light obvious. In CCD image sensors, laser can induce a so large electrons generation. Charges transfer inefficiency and charges blooming will cause the distortion of the charge distribution. Commonly, the largest signal outputted from CCD sensor is restricted by capability of the collection well of CCD, and can't go beyond the dynamic range for the subsequent electron circuits maintaining normal work. So the signal is not distorted in the post-processing circuits. But some techniques in the circuit can make some dazzling effects present different phenomenon in final image.

Occurrence, Distribution and Properties of Alfalfa Mosaic Virus

Directory of Open Access Journals (Sweden)

A.D. Zadjaii

2002-01-01

Full Text Available Alfalfa Mosaic Virus (AlflMV was recorded on 21 hosts comprising of four field crops, 14 vegetables, one ornamental plant and two new weed species (Heliotropium europaeum and Ammi majus belonging to nine families. The virus was identified and confirmed on the basis of its biological, serological (ELISA and physical properties. The leaves, stem and crown from systemically infected alfalfa plant contained high concentration of the virus. It was nonpersistently transmitted by cotton aphids (Aphis gossypii. The wide host range, including virus reservoirs, seed-borne infection and insect transmission account for high incidence and distribution of AlfMV in the country. The virus isolate had a dilution end point between 1 x 10-3 to l x 10-4, 65-67 °C thermal inactivation point and a few days in-vitro longevity and appears to be similar to the AlfMV-S strain.

Contribution of the Chromosomal ccdAB Operon to Bacterial Drug Tolerance.

Science.gov (United States)

Gupta, Kritika; Tripathi, Arti; Sahu, Alishan; Varadarajan, Raghavan

2017-10-01

One of the first identified and best-studied toxin-antitoxin (TA) systems in Escherichia coli is the F-plasmid-based CcdAB system. This system is involved in plasmid maintenance through postsegregational killing. More recently, ccdAB homologs have been found on the chromosome, including in pathogenic strains of E. coli and other bacteria. However, the functional role of chromosomal ccdAB genes, if any, has remained unclear. We show that both the native ccd operon of the E. coli O157 strain ( ccd O157 ) and the ccd operon from the F plasmid ( ccd F ), when inserted on the E. coli chromosome, lead to protection from cell death under multiple antibiotic stress conditions through formation of persisters, with the O157 operon showing higher protection. While the plasmid-encoded CcdB toxin is a potent gyrase inhibitor and leads to bacterial cell death even under fully repressed conditions, the chromosomally encoded toxin leads to growth inhibition, except at high expression levels, where some cell death is seen. This was further confirmed by transiently activating the chromosomal ccd operon through overexpression of an active-site inactive mutant of F-plasmid-encoded CcdB. Both the ccd F and ccd O157 operons may share common mechanisms for activation under stress conditions, eventually leading to multidrug-tolerant persister cells. This study clearly demonstrates an important role for chromosomal ccd systems in bacterial persistence. IMPORTANCE A large number of free-living and pathogenic bacteria are known to harbor multiple toxin-antitoxin systems, on plasmids as well as on chromosomes. The F-plasmid CcdAB system has been extensively studied and is known to be involved in plasmid maintenance. However, little is known about the function of its chromosomal counterpart, found in several pathogenic E. coli strains. We show that the native chromosomal ccd operon of the E. coli O157 strain is involved in drug tolerance and confers protection from cell death under multiple

Systems considerations in mosaic focal planes

Science.gov (United States)

White, K. P., III

1983-08-01

Two key reasons for pursuing the development of mosaic focal planes are reviewed and it is shown that rapid frame repetition rate is the only requirement that can be solved no other way than through mosaic focal planes. With the view that spaceborne mosaic focal plane sensors are necessarily 'smart sensors' requiring a lot of onboard processing just to function, it is pointed out that various artificial intelligence techniques may be the most appropriate to incorporate in the data processing. Finally, a novel mosaic focal plane design is proposed, termed a virtual mosaic focal plane, in response to other system constraints.

High-Voltage Clock Driver for Photon-Counting CCD Characterization

Science.gov (United States)

Baker, Robert

2013-01-01

A document discusses the CCD97 from e2v technologies as it is being evaluated at Goddard Space Flight Center's Detector Characterization Laboratory (DCL) for possible use in ultra-low background noise space astronomy applications, such as Terrestrial Planet Finder Coronagraph (TPF-C). The CCD97 includes a photoncounting mode where the equivalent output noise is less than one electron. Use of this mode requires a clock signal at a voltage level greater than the level achievable by the existing CCD (charge-coupled-device) electronics. A high-voltage waveform generator has been developed in code 660/601 to support the CCD97 evaluation. The unit generates required clock waveforms at voltage levels from -20 to +50 V. It deals with standard and arbitrary waveforms and supports pixel rates from 50 to 500 kHz. The system is designed to interface with existing Leach CCD electronics.

UVUDF: Ultraviolet imaging of the Hubble ultra deep field with wide-field camera 3

Energy Technology Data Exchange (ETDEWEB)

Teplitz, Harry I.; Rafelski, Marc; Colbert, James W.; Hanish, Daniel J. [Infrared Processing and Analysis Center, MS 100-22, Caltech, Pasadena, CA 91125 (United States); Kurczynski, Peter; Gawiser, Eric [Department of Physics and Astronomy, Rutgers University, Piscataway, NJ 08854 (United States); Bond, Nicholas A.; Gardner, Jonathan P.; De Mello, Duilia F. [Laboratory for Observational Cosmology, Astrophysics Science Division, Code 665, Goddard Space Flight Center, Greenbelt, MD 20771 (United States); Grogin, Norman; Koekemoer, Anton M.; Brown, Thomas M.; Coe, Dan; Ferguson, Henry C. [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218 (United States); Atek, Hakim [Laboratoire d' Astrophysique, École Polytechnique Fédérale de Lausanne (EPFL), Observatoire, CH-1290 Sauverny (Switzerland); Finkelstein, Steven L. [Department of Astronomy, The University of Texas at Austin, Austin, TX 78712 (United States); Giavalisco, Mauro [Astronomy Department, University of Massachusetts, Amherst, MA 01003 (United States); Gronwall, Caryl [Department of Astronomy and Astrophysics, The Pennsylvania State University, University Park, PA 16802 (United States); Lee, Kyoung-Soo [Department of Physics, Purdue University, 525 Northwestern Avenue, West Lafayette, IN 47907 (United States); Ravindranath, Swara, E-mail: hit@ipac.caltech.edu [Inter-University Centre for Astronomy and Astrophysics, Pune (India); and others

2013-12-01

We present an overview of a 90 orbit Hubble Space Telescope treasury program to obtain near-ultraviolet imaging of the Hubble Ultra Deep Field using the Wide Field Camera 3 UVIS detector with the F225W, F275W, and F336W filters. This survey is designed to: (1) investigate the episode of peak star formation activity in galaxies at 1 < z < 2.5; (2) probe the evolution of massive galaxies by resolving sub-galactic units (clumps); (3) examine the escape fraction of ionizing radiation from galaxies at z ∼ 2-3; (4) greatly improve the reliability of photometric redshift estimates; and (5) measure the star formation rate efficiency of neutral atomic-dominated hydrogen gas at z ∼ 1-3. In this overview paper, we describe the survey details and data reduction challenges, including both the necessity of specialized calibrations and the effects of charge transfer inefficiency. We provide a stark demonstration of the effects of charge transfer inefficiency on resultant data products, which when uncorrected, result in uncertain photometry, elongation of morphology in the readout direction, and loss of faint sources far from the readout. We agree with the STScI recommendation that future UVIS observations that require very sensitive measurements use the instrument's capability to add background light through a 'post-flash'. Preliminary results on number counts of UV-selected galaxies and morphology of galaxies at z ∼ 1 are presented. We find that the number density of UV dropouts at redshifts 1.7, 2.1, and 2.7 is largely consistent with the number predicted by published luminosity functions. We also confirm that the image mosaics have sufficient sensitivity and resolution to support the analysis of the evolution of star-forming clumps, reaching 28-29th magnitude depth at 5σ in a 0.''2 radius aperture depending on filter and observing epoch.

An LOD with improved breakdown voltage in full-frame CCD devices

Science.gov (United States)

Banghart, Edmund K.; Stevens, Eric G.; Doan, Hung Q.; Shepherd, John P.; Meisenzahl, Eric J.

2005-02-01

In full-frame image sensors, lateral overflow drain (LOD) structures are typically formed along the vertical CCD shift registers to provide a means for preventing charge blooming in the imager pixels. In a conventional LOD structure, the n-type LOD implant is made through the thin gate dielectric stack in the device active area and adjacent to the thick field oxidation that isolates the vertical CCD columns of the imager. In this paper, a novel LOD structure is described in which the n-type LOD impurities are placed directly under the field oxidation and are, therefore, electrically isolated from the gate electrodes. By reducing the electrical fields that cause breakdown at the silicon surface, this new structure permits a larger amount of n-type impurities to be implanted for the purpose of increasing the LOD conductivity. As a consequence of the improved conductance, the LOD width can be significantly reduced, enabling the design of higher resolution imaging arrays without sacrificing charge capacity in the pixels. Numerical simulations with MEDICI of the LOD leakage current are presented that identify the breakdown mechanism, while three-dimensional solutions to Poisson's equation are used to determine the charge capacity as a function of pixel dimension.

Deriving a per-field land use and land cover map in an agricultural mosaic catchment

Science.gov (United States)

Seo, B.; Bogner, C.; Poppenborg, P.; Martin, E.; Hoffmeister, M.; Jun, M.; Koellner, T.; Reineking, B.; Shope, C. L.; Tenhunen, J.

2014-09-01

Detailed data on land use and land cover constitute important information for Earth system models, environmental monitoring and ecosystem services research. Global land cover products are evolving rapidly; however, there is still a lack of information particularly for heterogeneous agricultural landscapes. We censused land use and land cover field by field in the agricultural mosaic catchment Haean in South Korea. We recorded the land cover types with additional information on agricultural practice. In this paper we introduce the data, their collection and the post-processing protocol. Furthermore, because it is important to quantitatively evaluate available land use and land cover products, we compared our data with the MODIS Land Cover Type product (MCD12Q1). During the studied period, a large portion of dry fields was converted to perennial crops. Compared to our data, the forested area was underrepresented and the agricultural area overrepresented in MCD12Q1. In addition, linear landscape elements such as waterbodies were missing in the MODIS product due to its coarse spatial resolution. The data presented here can be useful for earth science and ecosystem services research. The data are available at the public repository Pangaea (doi:110.1594/PANGAEA.823677).

The Keeling Curve and The Coral Reef Mosaic Project - Introducing the Realities of Climate Change to Educators and Scholars using Mosaic Arts.

Science.gov (United States)

Lueker, T.; Chinn, P. W. U.

2014-12-01

consequences of climate change and ocean acidification through field trips to NOAA research stations where the Keeling Curve and other climate change studies are conducted. This combination of field trips and mosaic workshops was sponsored in part by Christopher Sloop and Earth Networks (www.earthnetworks.com/OurNetworks/GreenhouseGasNetwork.aspx) . Fig.1

A compact CCD-monitored atomic force microscope with optical vision and improved performances.

Science.gov (United States)

Mingyue, Liu; Haijun, Zhang; Dongxian, Zhang

2013-09-01

A novel CCD-monitored atomic force microscope (AFM) with optical vision and improved performances has been developed. Compact optical paths are specifically devised for both tip-sample microscopic monitoring and cantilever's deflection detecting with minimized volume and optimal light-amplifying ratio. The ingeniously designed AFM probe with such optical paths enables quick and safe tip-sample approaching, convenient and effective tip-sample positioning, and high quality image scanning. An image stitching method is also developed to build a wider-range AFM image under monitoring. Experiments show that this AFM system can offer real-time optical vision for tip-sample monitoring with wide visual field and/or high lateral optical resolution by simply switching the objective; meanwhile, it has the elegant performances of nanometer resolution, high stability, and high scan speed. Furthermore, it is capable of conducting wider-range image measurement while keeping nanometer resolution. Copyright © 2013 Wiley Periodicals, Inc.

15 CFR 740.19 - Consumer Communications Devices (CCD).

Science.gov (United States)

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Consumer Communications Devices (CCD... EXCEPTIONS § 740.19 Consumer Communications Devices (CCD). (a) Authorization. This License Exception... controllers designed for chemical processing) designated EAR99; (4) Graphics accelerators and graphics...

Method to mosaic gratings that relies on analysis of far-field intensity patterns in two wavelengths

Science.gov (United States)

Hu, Yao; Zeng, Lijiang; Li, Lifeng

2007-01-01

We propose an experimental method to coherently mosaic two planar diffraction gratings. The method uses a Twyman-Green interferometer to guarantee the planar parallelism of the two sub-aperture gratings, and obtains the in-plane rotational error and the two translational errors from analysis of the far-field diffraction intensity patterns in two alignment wavelengths. We adjust the relative attitude and position of the two sub-aperture gratings to produce Airy disk diffraction patterns in both wavelengths. In our experiment, the repeatability of in-plane rotation adjustment was 2.35 μrad and that of longitudinal adjustment was 0.11 μm. The accuracy of lateral adjustment was about 2.9% of the grating period.

Evidence for RNA recombination between distinct isolates of Pepino mosaic virus.

NARCIS (Netherlands)

Hasiów-Jaroszewska, B.; Kuzniar, A.; Peters, S.A.; Leunissen, J.A.M.; Pospieszny, H.

2010-01-01

Genetic recombination plays an important role in the evolution of virus genomes. In this study we analyzed publicly available genomic sequences of Pepino mosaic virus (PepMV) for recombination events using several bioinformatics tools. The genome-wide analyses not only confirm the presence of

A method of fast mosaic for massive UAV images

Science.gov (United States)

Xiang, Ren; Sun, Min; Jiang, Cheng; Liu, Lei; Zheng, Hui; Li, Xiaodong

2014-11-01

With the development of UAV technology, UAVs are used widely in multiple fields such as agriculture, forest protection, mineral exploration, natural disaster management and surveillances of public security events. In contrast of traditional manned aerial remote sensing platforms, UAVs are cheaper and more flexible to use. So users can obtain massive image data with UAVs, but this requires a lot of time to process the image data, for example, Pix4UAV need approximately 10 hours to process 1000 images in a high performance PC. But disaster management and many other fields require quick respond which is hard to realize with massive image data. Aiming at improving the disadvantage of high time consumption and manual interaction, in this article a solution of fast UAV image stitching is raised. GPS and POS data are used to pre-process the original images from UAV, belts and relation between belts and images are recognized automatically by the program, in the same time useless images are picked out. This can boost the progress of finding match points between images. Levenberg-Marquard algorithm is improved so that parallel computing can be applied to shorten the time of global optimization notably. Besides traditional mosaic result, it can also generate superoverlay result for Google Earth, which can provide a fast and easy way to show the result data. In order to verify the feasibility of this method, a fast mosaic system of massive UAV images is developed, which is fully automated and no manual interaction is needed after original images and GPS data are provided. A test using 800 images of Kelan River in Xinjiang Province shows that this system can reduce 35%-50% time consumption in contrast of traditional methods, and increases respond speed of UAV image processing rapidly.

Active Pixel Sensors: Are CCD's Dinosaurs?

Science.gov (United States)

Fossum, Eric R.

1993-01-01

Charge-coupled devices (CCD's) are presently the technology of choice for most imaging applications. In the 23 years since their invention in 1970, they have evolved to a sophisticated level of performance. However, as with all technologies, we can be certain that they will be supplanted someday. In this paper, the Active Pixel Sensor (APS) technology is explored as a possible successor to the CCD. An active pixel is defined as a detector array technology that has at least one active transistor within the pixel unit cell. The APS eliminates the need for nearly perfect charge transfer -- the Achilles' heel of CCDs. This perfect charge transfer makes CCD's radiation 'soft,' difficult to use under low light conditions, difficult to manufacture in large array sizes, difficult to integrate with on-chip electronics, difficult to use at low temperatures, difficult to use at high frame rates, and difficult to manufacture in non-silicon materials that extend wavelength response.

Wide-field schematic eye models with gradient-index lens.

Science.gov (United States)

Goncharov, Alexander V; Dainty, Chris

2007-08-01

We propose a wide-field schematic eye model, which provides a more realistic description of the optical system of the eye in relation to its anatomical structure. The wide-field model incorporates a gradient-index (GRIN) lens, which enables it to fulfill properties of two well-known schematic eye models, namely, Navarro's model for off-axis aberrations and Thibos's chromatic on-axis model (the Indiana eye). These two models are based on extensive experimental data, which makes the derived wide-field eye model also consistent with that data. A mathematical method to construct a GRIN lens with its iso-indicial contours following the optical surfaces of given asphericity is presented. The efficiency of the method is demonstrated with three variants related to different age groups. The role of the GRIN structure in relation to the lens paradox is analyzed. The wide-field model with a GRIN lens can be used as a starting design for the eye inverse problem, i.e., reconstructing the optical structure of the eye from off-axis wavefront measurements. Anatomically more accurate age-dependent optical models of the eye could ultimately help an optical designer to improve wide-field retinal imaging.

Cooling System for a Frame-Store PN-CCD Detector for Low Background Application

CERN Document Server

Pereira, H; Santos Silva, P; Kuster, M; Lang, P

2012-01-01

The astroparticle physics experiment CERN Axion Solar Telescope (CAST) aims to detect hypothetical axions or axion-like particles produced in the Sun by the Primakoff process. A Large Hadron Collider (LHC) prototype superconducting dipole magnet provides a 9 T transverse magnetic field for the conversion of axions into detectable X-ray photons. These photons are detected with an X-ray telescope and a novel type of frame-store CCD detector built from radio-pure materials, installed in the optics focal plane. A novel type of cooling system has been designed and built based on krypton-filled cryogenic heat pipes, made out of oxygen-free radiopure copper, and a Stirling cryocooler as cold source. The heat pipes provide an efficient thermal coupling between the cryocooler and the CCD which is kept at stable temperatures between 150 and 230 K within an accuracy of 0.1 K. A graded-Z radiation shield, also serving as a gas cold-trap operated at 120 K, is implemented to reduce the surface contamination of the CCD wind...

A Foxtail mosaic virus Vector for Virus-Induced Gene Silencing in Maize.

Science.gov (United States)

Mei, Yu; Zhang, Chunquan; Kernodle, Bliss M; Hill, John H; Whitham, Steven A

2016-06-01

Plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing (VIGS). A limited number of viruses have been developed into viral vectors for the purposes of gene expression or VIGS in monocotyledonous plants, and among these, the tripartite viruses Brome mosaic virus and Cucumber mosaic virus have been shown to induce VIGS in maize (Zea mays). We describe here a new DNA-based VIGS system derived from Foxtail mosaic virus (FoMV), a monopartite virus that is able to establish systemic infection and silencing of endogenous maize genes homologous to gene fragments inserted into the FoMV genome. To demonstrate VIGS applications of this FoMV vector system, four genes, phytoene desaturase (functions in carotenoid biosynthesis), lesion mimic22 (encodes a key enzyme of the porphyrin pathway), iojap (functions in plastid development), and brown midrib3 (caffeic acid O-methyltransferase), were silenced and characterized in the sweet corn line Golden × Bantam. Furthermore, we demonstrate that the FoMV infectious clone establishes systemic infection in maize inbred lines, sorghum (Sorghum bicolor), and green foxtail (Setaria viridis), indicating the potential wide applications of this viral vector system for functional genomics studies in maize and other monocots. © 2016 American Society of Plant Biologists. All Rights Reserved.

A USB 2.0 computer interface for the UCO/Lick CCD cameras

Science.gov (United States)

Wei, Mingzhi; Stover, Richard J.

2004-09-01

The new UCO/Lick Observatory CCD camera uses a 200 MHz fiber optic cable to transmit image data and an RS232 serial line for low speed bidirectional command and control. Increasingly RS232 is a legacy interface supported on fewer computers. The fiber optic cable requires either a custom interface board that is plugged into the mainboard of the image acquisition computer to accept the fiber directly or an interface converter that translates the fiber data onto a widely used standard interface. We present here a simple USB 2.0 interface for the UCO/Lick camera. A single USB cable connects to the image acquisition computer and the camera's RS232 serial and fiber optic cables plug into the USB interface. Since most computers now support USB 2.0 the Lick interface makes it possible to use the camera on essentially any modern computer that has the supporting software. No hardware modifications or additions to the computer are needed. The necessary device driver software has been written for the Linux operating system which is now widely used at Lick Observatory. The complete data acquisition software for the Lick CCD camera is running on a variety of PC style computers as well as an HP laptop.

A wide field X-ray camera

International Nuclear Information System (INIS)

Sims, M.; Turner, M.J.L.; Willingale, R.

1980-01-01

A wide field of view X-ray camera based on the Dicke or Coded Mask principle is described. It is shown that this type of instrument is more sensitive than a pin-hole camera, or than a scanning survey of a given region of sky for all wide field conditions. The design of a practical camera is discussed and the sensitivity and performance of the chosen design are evaluated by means of computer simulations. The Wiener Filter and Maximum Entropy methods of deconvolution are described and these methods are compared with each other and cross-correlation using data from the computer simulations. It is shown that the analytic expressions for sensitivity used by other workers are confirmed by the simulations, and that ghost images caused by incomplete coding can be substantially eliminated by the use of the Wiener Filter and the Maximum Entropy Method, with some penalty in computer time for the latter. The cyclic mask configuration is compared with the simple mask camera. It is shown that when the diffuse X-ray background dominates, the simple system is more sensitive and has the better angular resolution. When sources dominate the simple system is less sensitive. It is concluded that the simple coded mask camera is the best instrument for wide field imaging of the X-ray sky. (orig.)

GENERATION OF GEOMETRIC ORNAMENTS IN ANCIENT MOSAIC ART

Directory of Open Access Journals (Sweden)

SASS Ludmila

2015-06-01

Full Text Available The paper examines geometrical ornaments from ancient mosaic.We studied the geometric generation by using Computer Aided Graphics for three examples of ancient mosaic: a mosaic of Ancient Corinth, a mosaic of the sacred geometry Flower of Life (exposed in the National Museum of Israel and a mosaic of fortress Masada - Israel. The technique of drawing ancient mosaic is recomposed using computer aided graphics. A program has been developed that can help draw a petal-type arc (semicircle of the mosaic that is the Byzantine church of Masada. Based on these mosaics, other variants of aesthetic images in monochrome or black and white and polychrome were drawn, all of which can be materialized in decorative art to embellish various surfaces: walls, floors, pools, fountains, etc.

WATERMELON MOSAIC VIRUS OF PUMPKIN (Cucurbita maxima FROM SULAWESI: IDENTIFICATION, TRANSMISSION, AND HOST RANGE

Directory of Open Access Journals (Sweden)

Wasmo Wakmana

2016-10-01

Full Text Available A mosaic disease of pumpkin (Cucurbita maxima was spread widely in Sulawesi. Since the virus had not yet been identified, a study was conducted to identify the disease through mechanical inoculation, aphid vector transmission, host range, and electron microscopic test. Crude sap of infected pumpkin leaf samples was rubbed on the cotyledons of healthy pumpkin seedlings for mechanical inoculation. For insect transmission, five infective aphids were infected per seedling. Seedlings of eleven different species were inoculated mechanically for host range test. Clarified sap was examined under the electron microscope. Seeds of two pumpkin fruits from two different infected plants were planted and observed for disease transmission up to one-month old seedlings. The mosaic disease was transmitted mechanically from crude sap of different leaf samples to healthy pumpkin seedlings showing mosaic symptoms. The virus also infected eight cucurbits, i.e., cucumber (Cucumis sativus, green melon (Cucumis melo, orange/rock melon (C. melo, zucchini (Cucurbita pepo, pumpkin (Cucurbita maxima, water melon (Citrulus vulgaris, Bennicosa hispida, and blewah (Cucurbita sp.. Aphids  transmitted the disease from one to other pumpkin seedlings. The virus was not transmitted by seed. The mosaic disease of pumpkin at Maros, South Sulawesi, was associated with flexious particles of approximately 750 nm length, possibly a potyvirus, such as water melon mosaic virus rather than papaya ringspot virus or zucchini yellow mosaic virus.

Transcriptome sequencing of two phenotypic mosaic Eucalyptus trees reveals large scale transcriptome re-modelling.

Directory of Open Access Journals (Sweden)

Amanda Padovan

Full Text Available Phenotypic mosaic trees offer an ideal system for studying differential gene expression. We have investigated two mosaic eucalypt trees from two closely related species (Eucalyptus melliodora and E. sideroxylon, which each support two types of leaves: one part of the canopy is resistant to insect herbivory and the remaining leaves are susceptible. Driving this ecological distinction are differences in plant secondary metabolites. We used these phenotypic mosaics to investigate genome wide patterns of foliar gene expression with the aim of identifying patterns of differential gene expression and the somatic mutation(s that lead to this phenotypic mosaicism. We sequenced the mRNA pool from leaves of the resistant and susceptible ecotypes from both mosaic eucalypts using the Illumina HiSeq 2000 platform. We found large differences in pathway regulation and gene expression between the ecotypes of each mosaic. The expression of the genes in the MVA and MEP pathways is reflected by variation in leaf chemistry, however this is not the case for the terpene synthases. Apart from the terpene biosynthetic pathway, there are several other metabolic pathways that are differentially regulated between the two ecotypes, suggesting there is much more phenotypic diversity than has been described. Despite the close relationship between the two species, they show large differences in the global patterns of gene and pathway regulation.

Solution structure and elevator mechanism of the membrane electron transporter CcdA.

Science.gov (United States)

Zhou, Yunpeng; Bushweller, John H

2018-02-01

Membrane oxidoreductase CcdA plays a central role in supplying reducing equivalents from the bacterial cytoplasm to the envelope. It transports electrons across the membrane using a single pair of cysteines by a mechanism that has not yet been elucidated. Here we report an NMR structure of the Thermus thermophilus CcdA (TtCcdA) in an oxidized and outward-facing state. CcdA consists of two inverted structural repeats of three transmembrane helices (2 × 3-TM). We computationally modeled and experimentally validated an inward-facing state, which suggests that CcdA uses an elevator-type movement to shuttle the reactive cysteines across the membrane. CcdA belongs to the LysE superfamily, and thus its structure may be relevant to other LysE clan transporters. Structure comparisons of CcdA, semiSWEET, Pnu, and major facilitator superfamily (MFS) transporters provide insights into membrane transporter architecture and mechanism.

The Brazilian wide field imaging camera (WFI) for the China/Brazil earth resources satellite: CBERS 3 and 4

Science.gov (United States)

Scaduto, L. C. N.; Carvalho, E. G.; Modugno, R. G.; Cartolano, R.; Evangelista, S. H.; Segoria, D.; Santos, A. G.; Stefani, M. A.; Castro Neto, J. C.

2017-11-01

The purpose of this paper is to present the optical system developed for the Wide Field imaging Camera - WFI that will be integrated to the CBERS 3 and 4 satellites (China Brazil Earth resources Satellite). This camera will be used for remote sensing of the Earth and it is aimed to work at an altitude of 778 km. The optical system is designed for four spectral bands covering the range of wavelengths from blue to near infrared and its field of view is +/-28.63°, which covers 866 km, with a ground resolution of 64 m at nadir. WFI has been developed through a consortium formed by Opto Electrônica S. A. and Equatorial Sistemas. In particular, we will present the optical analysis based on the Modulation Transfer Function (MTF) obtained during the Engineering Model phase (EM) and the optical tests performed to evaluate the requirements. Measurements of the optical system MTF have been performed using an interferometer at the wavelength of 632.8nm and global MTF tests (including the CCD and signal processing electronic) have been performed by using a collimator with a slit target. The obtained results showed that the performance of the optical system meets the requirements of project.

Michelson wide-field stellar interferometry : Principles and experimental verification

NARCIS (Netherlands)

Montilla, I.; Pereira, S.F.; Braat, J.J.M.

2005-01-01

A new interferometric technique for Michelson wide-field interferometry is presented that consists of a Michelson pupil-plane combination scheme in which a wide field of view can be achieved in one shot. This technique uses a stair-shaped mirror in the intermediate image plane of each telescope in

CCD camera eases the control of a soda recovery boiler; CCD-kamera helpottaa soodakattilan valvontaa

Energy Technology Data Exchange (ETDEWEB)

Kinnunen, L.

2001-07-01

Fortum Technology has developed a CCD firebox camera, based on semiconductor technology, enduring hard conditions of soda recovery boiler longer than traditional cameras. The firebox camera air- cooled and the same air is pressed over the main lens so it remains clean despite of the alkaline liquor splashing around in the boiler. The image of the boiler is transferred through the main lens, image transfer lens and a special filter, mounted inside the camera tube, into the CCD camera. The first CCD camera system has been in use since 1999 in Sunila pulp mill in Kotka, owned by Myllykoski Oy and Enso Oyj. The mill has two medium-sized soda recovery boilers. The amount of black liquor, formed daily, is about 2000 tons DS, which is more than enough for the heat generation. Even electric power generation exceeds sometimes the demand, so the surplus power can be sold out. Black liquor is sprayed inside the soda recovery boiler with high pressure. The liquor form droplets in the boiler, the temperature of which is over 1000 deg C. A full-hot pile is formed at the bottom of the boiler after burning. The size and shape of the pile effect on the efficiency and the emissions of the boiler. The camera has operated well.

Lensless imaging for wide field of view

Science.gov (United States)

Nagahara, Hajime; Yagi, Yasushi

2015-02-01

It is desirable to engineer a small camera with a wide field of view (FOV) because of current developments in the field of wearable cameras and computing products, such as action cameras and Google Glass. However, typical approaches for achieving wide FOV, such as attaching a fisheye lens and convex mirrors, require a trade-off between optics size and the FOV. We propose camera optics that achieve a wide FOV, and are at the same time small and lightweight. The proposed optics are a completely lensless and catoptric design. They contain four mirrors, two for wide viewing, and two for focusing the image on the camera sensor. The proposed optics are simple and can be simply miniaturized, since we use only mirrors for the proposed optics and the optics are not susceptible to chromatic aberration. We have implemented the prototype optics of our lensless concept. We have attached the optics to commercial charge-coupled device/complementary metal oxide semiconductor cameras and conducted experiments to evaluate the feasibility of our proposed optics.

CCD camera system for use with a streamer chamber

International Nuclear Information System (INIS)

Angius, S.A.; Au, R.; Crawley, G.C.; Djalali, C.; Fox, R.; Maier, M.; Ogilvie, C.A.; Molen, A. van der; Westfall, G.D.; Tickle, R.S.

1988-01-01

A system based on three charge-coupled-device (CCD) cameras is described here. It has been used to acquire images from a streamer chamber and consists of three identical subsystems, one for each camera. Each subsystem contains an optical lens, CCD camera head, camera controller, an interface between the CCD and a microprocessor, and a link to a minicomputer for data recording and on-line analysis. Image analysis techniques have been developed to enhance the quality of the particle tracks. Some steps have been made to automatically identify tracks and reconstruct the event. (orig.)

Defect inspection in hot slab surface: multi-source CCD imaging based fuzzy-rough sets method

Science.gov (United States)

Zhao, Liming; Zhang, Yi; Xu, Xiaodong; Xiao, Hong; Huang, Chao

2016-09-01

To provide an accurate surface defects inspection method and make the automation of robust image region of interests(ROI) delineation strategy a reality in production line, a multi-source CCD imaging based fuzzy-rough sets method is proposed for hot slab surface quality assessment. The applicability of the presented method and the devised system are mainly tied to the surface quality inspection for strip, billet and slab surface etcetera. In this work we take into account the complementary advantages in two common machine vision (MV) systems(line array CCD traditional scanning imaging (LS-imaging) and area array CCD laser three-dimensional (3D) scanning imaging (AL-imaging)), and through establishing the model of fuzzy-rough sets in the detection system the seeds for relative fuzzy connectedness(RFC) delineation for ROI can placed adaptively, which introduces the upper and lower approximation sets for RIO definition, and by which the boundary region can be delineated by RFC region competitive classification mechanism. For the first time, a Multi-source CCD imaging based fuzzy-rough sets strategy is attempted for CC-slab surface defects inspection that allows an automatic way of AI algorithms and powerful ROI delineation strategies to be applied to the MV inspection field.

Dynamic Mapping of Rice Growth Parameters Using HJ-1 CCD Time Series Data

Directory of Open Access Journals (Sweden)

Jing Wang

2016-11-01

Full Text Available The high temporal resolution (4-day charge-coupled device (CCD cameras onboard small environment and disaster monitoring and forecasting satellites (HJ-1A/B with 30 m spatial resolution and large swath (700 km have substantially increased the availability of regional clear sky optical remote sensing data. For the application of dynamic mapping of rice growth parameters, leaf area index (LAI and aboveground biomass (AGB were considered as plant growth indicators. The HJ-1 CCD-derived vegetation indices (VIs showed robust relationships with rice growth parameters. Cumulative VIs showed strong performance for the estimation of total dry AGB. The cross-validation coefficient of determination ( R C V 2 was increased by using two machine learning methods, i.e., a back propagation neural network (BPNN and a support vector machine (SVM compared with traditional regression equations of LAI retrieval. The LAI inversion accuracy was further improved by dividing the rice growth period into before and after heading stages. This study demonstrated that continuous rice growth monitoring over time and space at field level can be implemented effectively with HJ-1 CCD 10-day composite data using a combination of proper VIs and regression models.

Controlled transmission of African cassava mosaic virus (ACMV) by ...

African Journals Online (AJOL)

Jatropha curcas, a plant with great biodiesel potential is also used to reduce the population of whiteflies, Bemisia tabaci on cassava fields when planted as a hedge. We therefore, investigated the transmission of African cassava mosaic virus (ACMV) by the whitefly vector from cassava to seedlings of 10 accessions of J.

Review The Ooty Wide Field Array

Indian Academy of Sciences (India)

c Indian Academy of Sciences. DOI 10.1007/s12036-017-9430-4. Review. The Ooty Wide Field ... salient features of the upgrade, as well as its main science drivers. There are three ..... tecture for low frequency arrays, Ph.D. thesis, Jawaharalal.

WFIRST: Astrometry with the Wide-Field Imager

Science.gov (United States)

Bellini, Andrea; WFIRST Astrometry Working Group

2018-01-01

The wide field of view and stable, sharp images delivered by WFIRST's Wide-Field Imager make it an excellent instrument for astrometry, one of five major discovery areas identified in the 2010 Decadal Survey. Compared to the Hubble Space Telescope, WFIRST's wider field of view with similar image quality will provide hundreds more astrometric targets per image as well as background galaxies and stars with precise positions in the Gaia catalog. In addition, WFIRST will operate in the infrared, a wavelength regime where the most precise astrometry has so far been achieved with adaptive optics images from large ground-based telescopes. WFIRST will provide at least a factor of three improvement in astrometry over the current state of the art in this wavelength range, while spanning a field of view thousands of times larger. WFIRST is thus poised to make major contributions to multiple science topics in which astrometry plays an important role, without major alterations to the planned mission or instrument. We summarize a few of the most compelling science cases where WFIRST astrometry could prove transformational.

Application of the CCD Fabry-Perot Annular Summing Technique to Thermospheric O(1)D.

Science.gov (United States)

Coakley, Monica Marie

1995-01-01

This work will detail the verification of the advantages of the Fabry-Perot charge coupled device (CCD) annular summing technique, the development of the technique for analysis of daysky spectra, and the implications of the resulting spectra for neutral temperature and wind measurements in the daysky thermosphere. The daysky spectral feature of interest is the bright (1 kilo-Rayleigh) thermospheric (OI) emission at 6300 A which had been observed in the nightsky in order to determine winds and temperatures in the vicinity of the altitude of 250 km. In the daysky, the emission line sits on top of a bright Rayleigh scattered continuum background which significantly complicates the observation. With a triple etalon Fabry-Perot spectrometer, the continuum background can be reduced while maintaining high throughput and high resolution. The inclusion of a CCD camera results in significant savings in integration time over the two more standard scanning photomultiplier systems that have made the same wind and temperature measurements in the past. A comparable CCD system can experience an order of magnitude savings in integration time over a PMT system. Laboratory and field tests which address the advantages and limitations of both the Fabry-Perot CCD annular summing technique and the daysky CCD imaging are included in Chap. 2 and Chap. 3. With a sufficiently large throughput associated with the spectrometer and a CCD detector, rapid observations (~4 minute integrations) can be made. Extraction of the line width and line center from the daysky near-continuum background is complicated compared to the nightsky case, but possible. Methods of fitting the line are included in Chap. 4. The daysky O ^1D temperatures are consistent with a lower average emission height than predicted by models. The data and models are discussed in Chap. 5. Although some discrepancies exist between resulting temperatures and models, the observations indicate the potential for other direct measurements

CCD-based X-ray detectors for X-ray diffraction studies

International Nuclear Information System (INIS)

Ito, K.; Amemiya, Y.

1999-01-01

CCD-based X-ray detectors are getting to be used for X-ray diffraction studies especially in the studies where real time (automated) measurements and time-resolved measurements are required. Principles and designs of two typical types of CCD-based detectors are described; one is ths system in which x-ray image intensifiers are coupled to maximize the detective quantum efficiency for time-resolved measurements, and the other is the system in which tapered optical fibers are coupled for the reduction of the image into the CCD, which is optimized for automated measurements for protein crystallography. These CCD-based X-ray detectors have an image distortion and non-uniformity of response to be corrected by software. Correction schemes which we have developed are also described. (author)

Image differencing using masked CCD

International Nuclear Information System (INIS)

Rushbrooke, J.G.; Ansorge, R.E.; Webber, C.J. St. J.

1987-01-01

A charge coupled device has some of its ''pixels'' masked by a material which is opaque to the radiation to which the device is to be exposed, each masked region being employed as a storage zone into which the charge pattern from the unmasked pixels can be transferred to enable a subsequent charge pattern to be established on further exposure of the unmasked pixels. The components of the resulting video signal corresponding to the respective charge patterns read-out from the CCD are subtracted to produce a video signal corresponding to the difference between the two images which formed the respective charge patterns. Alternate rows of pixels may be masked, or chequer-board pattern masking may be employed. In an X-ray imaging system the CCD is coupled to image intensifying and converting means. (author)

A CCD portrait of Comet P/Tempel 2

International Nuclear Information System (INIS)

Jewitt, D.; Luu, J.

1989-01-01

The development of activity in Comet P/Tempel 2 is studied from aphelion (R = 4 AU) to perihelion (R = 1.4 AU) using extensive time-series CCD photometry and CCD spectra. The comet undergoes a profound morphological change at R of about 2-2.5 AU, from a bare nucleus at larger distances to an active comet supporting a coma of gas and dust. Cyclic photometric variations with the period T = 8.95 + or - 0.01 hr. are present at all R, and are attributed to the rotation of the nucleus at this period. The nucleus is prolate (axes a:b:c = 1.9:1:1), a property shared with other nuclei studied using CCD photometry. Novel results include a limit on the bulk density of the nucleus, rho above 300 kg/cu m, and a 20-A-resolution CCD spectrum of the nucleus. Spatially and temporally resolved photometry is used to study the effects of nucleus rotation on the coma. The coma does not share the dramatic photometric variations shown by the nucleus. It possesses a steep surface-brightness distribution, which is attributable to progressive destruction of the coma grains with increasing space exposure. 41 refs

Programmable CCD imaging system for synchrotron radiation studies

International Nuclear Information System (INIS)

Rodricks, B.; Brizard, C.

1992-01-01

A real-time imaging system for x-ray detection has been developed. The CAMAC-based system has a Charge Coupled Device (CCD) as its active detection element. The electronics consist of a CAMAC-crate-based dedicated microprocessor coupled to arbitrary waveform generators, programmable timing, and ADC modules. The hardware flexibility achievable through this system enables one to use virtually any commercially available CCD. A dedicated CAMAC-based display driver allows for real-time imaging on a high-resolution color monitor. An optional front end consisting of a fiber-optic taper and a focusing optical lens system coupled to a phosphor screen allows for large area imaging. Further, programming flexibility, in which the detector can be used in different read-out modes, enables it to be exploited for time-resolved experiments. In one mode, sections of the CCD can be read-out with millisecond time-resolution and, in another, the use of the CCD as a storage device is exploited resulting in microsecond time-resolution. Three different CCDs with radically different read-out timings and waveforms have been tested: the TI 4849, a 39Ox584 pixel array; TC 215, a 1024x1O24 pixel array; and the TH 7883, a 576x384 pixel array. The TC 215 and TI 4849 are single-phase CCDs manufactured by Texas Instruments, and the TH 7883 is a four-phase device manufactured by Thomson-CSF. The CCD characterized for uniformity, charge transfer efficiency (CTE), linearity, and sensitivity is the TC215

Stray-field-induced Faraday contributions in wide-field Kerr microscopy and -magnetometry

International Nuclear Information System (INIS)

Markó, D.; Soldatov, I.; Tekielak, M.; Schäfer, R.

2015-01-01

The magnetic domain contrast in wide-field Kerr microscopy on bulk specimens can be substantially distorted by non-linear, field-dependent Faraday rotations in the objective lens that are caused by stray-field components emerging from the specimen. These Faraday contributions, which were detected by Kerr-magnetometry on grain-oriented iron–silicon steel samples, are thoroughly elaborated and characterized. They express themselves as a field-dependent gray-scale offset to the domain contrast and in highly distorted surface magnetization curves if optically measured in a wide field Kerr microscope. An experimental method to avoid such distortions is suggested. In the course of these studies, a low-permeability part in the surface magnetization loop of slightly misoriented (110)-surfaces in iron–silicon sheets was discovered that is attributed to demagnetization effects in direction perpendicular to the sheet surface. - Highlights: • Magnetizing a finite sample in a Kerr microscope leads to sample-generated stray-fields. • They cause non-linear, field- and position-dependent Faraday rotations in the objective. • This leads to a modulation of the Kerr contrast and to distorted MOKE loops. • A method to compensate these Faraday rotations is presented

Characterization of a pnCCD for applications with synchrotron radiation

Energy Technology Data Exchange (ETDEWEB)

Send, S., E-mail: send@physik.uni-siegen.de [University of Siegen, Department of Physics, Walter-Flex-Straße 3, 57068 Siegen (Germany); Abboud, A. [University of Siegen, Department of Physics, Walter-Flex-Straße 3, 57068 Siegen (Germany); Hartmann, R.; Huth, M. [PNSensor GmbH, Römerstraße 28, 80803 München (Germany); Leitenberger, W. [University of Potsdam, Department of Physics, Karl-Liebknecht-Straße 24/25, 14476 Potsdam (Germany); Pashniak, N. [University of Siegen, Department of Physics, Walter-Flex-Straße 3, 57068 Siegen (Germany); Schmidt, J. [PNSensor GmbH, Römerstraße 28, 80803 München (Germany); Strüder, L. [University of Siegen, Department of Physics, Walter-Flex-Straße 3, 57068 Siegen (Germany); PNSensor GmbH, Römerstraße 28, 80803 München (Germany); Max-Planck-Institut für extraterrestrische Physik, Giessenbachstraße, 85748 Garching (Germany); Pietsch, U. [University of Siegen, Department of Physics, Walter-Flex-Straße 3, 57068 Siegen (Germany)

2013-05-21

In this work we study the response of a pnCCD by means of X-ray spectroscopy in the energy range between 6 keV and 20 keV and by Laue diffraction techniques. The analyses include measurements of characteristic detector parameters like energy resolution, count rate capability and effects of different gain settings. The limit of a single photon counting operation in white beam X-ray diffraction experiments is discussed with regard to the occurrence of pile-up events, for which the energy information about individual photons is lost. In case of monochromatic illumination the pnCCD can be used as a fast conventional CCD with a charge handling capacity (CHC) of about 300,000 electrons per pixel. If the CHC is exceeded, any surplus charge will spill to neighboring pixels perpendicular to the transfer direction due to electrostatic repulsion. The possibilities of increasing the number of storable electrons are investigated for different voltage settings by exposing a single pixel with X-rays generated by a microfocus X-ray source. The pixel binning mode is tested as an alternative approach that enables a pnCCD operation with significantly shorter readout times. -- Highlights: ► The pnCCD acts as a four-dimensional detector for white X-rays. ► Its performance for applications with synchrotron radiation is investigated. ► The pnCCD can be used for single photon counting and photon integration. ► The operation mode depends on the local frequencies of pile-up events. ► The pnCCD can be optimized for X-ray spectroscopy and X-ray imaging.

Tubule-forming capacity of the movement proteins of alfalfa mosaic virus and brome mosaic virus

NARCIS (Netherlands)

Kasteel, D. T.; van der Wel, N. N.; Jansen, K. A.; Goldbach, R. W.; van Lent, J. W.

1997-01-01

The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a

BV RI CCD photometry of 361,281 objects in the field of M 31

Science.gov (United States)

Magnier, E. A.; Lewin, W. H. G.; Van Paradijs, J.; Hasinger, G.; Jain, A.; Pietsch, W.; Truemper, J.

1992-01-01

Deep BV RI CCD photometry was performed on a 1 sq deg region of M 31. A catalog of photometry and astrometry of a total of 361,281 stars is presented, with typical completion limits of BV RI = (22.3, 22.2, 22.2, 20.9). Photometric accuracy is about 2 percent at V = 19. This catalog allows detailed studies of stellar populations and reddening. The data are currently being used to assist in finding the optical counterparts of Einstein and ROSAT X-ray sources.

Impact of yam mosaic virus (YMV) on tuber yield of white yam ...

African Journals Online (AJOL)

The impact of Yam mosaic virus (YMV) on tuber yield of white yam (Dioscorea rotundata Poir) in the forest zone of Ghana was assessed under controlled environment and in the field at Kumasi, Ghana. Two field trials were carried out in 2001 and 2002. The experiment under controlled environment was conducted in 2003.

Software design of control system of CCD side-scatter lidar

Science.gov (United States)

Kuang, Zhiqiang; Liu, Dong; Deng, Qian; Zhang, Zhanye; Wang, Zhenzhu; Yu, Siqi; Tao, Zongming; Xie, Chenbo; Wang, Yingjian

2018-03-01

Because of the existence of blind zone and transition zone, the application of backscattering lidar in near-ground is limited. The side-scatter lidar equipped with the Charge Coupled Devices (CCD) can separate the transmitting and receiving devices to avoid the impact of the geometric factors which is exited in the backscattering lidar and, detect the more precise near-ground aerosol signals continuously. Theories of CCD side-scatter lidar and the design of control system are introduced. The visible control of laser and CCD and automatic data processing method of the side-scatter lidar are developed by using the software of Visual C #. The results which are compared with the calibration of the atmospheric aerosol lidar data show that signals from the CCD side- scatter lidar are convincible.

Honeywell's Compact, Wide-angle Uv-visible Imaging Sensor

Science.gov (United States)

Pledger, D.; Billing-Ross, J.

1993-01-01

Honeywell is currently developing the Earth Reference Attitude Determination System (ERADS). ERADS determines attitude by imaging the entire Earth's limb and a ring of the adjacent star field in the 2800-3000 A band of the ultraviolet. This is achieved through the use of a highly nonconventional optical system, an intensifier tube, and a mega-element CCD array. The optics image a 30 degree region in the center of the field, and an outer region typically from 128 to 148 degrees, which can be adjusted up to 180 degrees. Because of the design employed, the illumination at the outer edge of the field is only some 15 percent below that at the center, in contrast to the drastic rolloffs encountered in conventional wide-angle sensors. The outer diameter of the sensor is only 3 in; the volume and weight of the entire system, including processor, are 1000 cc and 6 kg, respectively.

A new approach to modelling radiation noise in CCD's

International Nuclear Information System (INIS)

Chugg, A.; Hopkinson, G.

1998-01-01

The energy depositions reported by Monte Carlo electron-photon irradiation transport codes are subject to a random error due to the finite number of particle histories used to generate the results. These statistical variations, normally a nuisance, may also be identified with the real radiation noise effects experienced by CCD pixels in persistent radiation environments. This paper explores the practicability of such radiation noise modelling by applying the ACCEPT code from the ITS suite to the case of a shielded CCD exposed to an electron flux. The results are compared with those obtained in a subsequent electron irradiation of the CCD by a Van de Graaff accelerator

The interaction of DNA gyrase with the bacterial toxin CcdB

DEFF Research Database (Denmark)

Kampranis, S C; Howells, A J; Maxwell, A

1999-01-01

CcdB is a bacterial toxin that targets DNA gyrase. Analysis of the interaction of CcdB with gyrase reveals two distinct complexes. An initial complex (alpha) is formed by direct interaction between GyrA and CcdB; this complex can be detected by affinity column and gel-shift analysis, and has...... of this initial complex with ATP in the presence of GyrB and DNA slowly converts it to a second complex (beta), which has a lower rate of ATP hydrolysis and is unable to catalyse supercoiling. The efficiency of formation of this inactive complex is dependent on the concentrations of ATP and CcdB. We suggest...

Discriminação de variedades de citros em imagens CCD/CBERS-2 Discrimination of citrus varieties using CCD/CBERS-2 satellite imagery

Directory of Open Access Journals (Sweden)

Ieda Del'Arco Sanches

2008-02-01

Full Text Available O presente trabalho teve o objetivo de avaliar as imagens CCD/CBERS-2 quanto à possibilidade de discriminarem variedades de citros. A área de estudo localiza-se em Itirapina (SP e, para este estudo, foram utilizadas imagens CCD de três datas (30/05/2004, 16/08/2004 e 11/09/2004. Um modelo que integra os elementos componentes da cena citrícola sensoriada é proposto com o objetivo de explicar a variabilidade das respostas das parcelas de citros em imagens orbitais do tipo CCD/CBERS-2. Foram feitas classificações pelos algoritmos Isoseg e Maxver e, de acordo com o índice kappa, concluiu-se que é possível obterem-se exatidões qualificadas como muito boas, sendo que as melhores classificações foram conseguidas com imagens da estação seca.This paper was aimed at evaluating the possibility of discriminating citrus varieties in CCD imageries from CBERS-2 satellite ("China-Brazil Earth Resouces Satellite". The study area is located in Itirapina, São Paulo State. For this study, three CCD images from 2004 were acquired (May 30, August 16, and September 11. In order to acquire a better understanding and for explaining the variability of the spectral behavior of the citrus areas in orbital images (like as the CCD/CBERS-2 images a model that integrates the elements of the citrus scene is proposed and discussed. The images were classified by Isoseg and MaxVer classifiers. According to kappa index, it was possible to obtain classifications qualified as 'very good'. The best results were obtained with the images from the dry season.

Advanced MOKE magnetometry in wide-field Kerr-microscopy

Science.gov (United States)

Soldatov, I. V.; Schäfer, R.

2017-10-01

The measurement of MOKE (Magneto-Optical Kerr Effect) magnetization loops in a wide-field Kerr microscope offers the advantage that the relevant domain images along the loop can be readily recorded. As the microscope's objective lens is exposed to the magnetic field, the loops are usually strongly distorted by non-linear Faraday rotations of the polarized light that occur in the objective lens and that are superimposed to the MOKE signal. In this paper, an experimental method, based on a motorized analyzer, is introduced which allows to compensate the Faraday contributions, thus leading to pure MOKE loops. A wide field Kerr microscope, equipped with this technology, works well as a laser-based MOKE magnetometer, additionally offering domain images and thus providing the basis for loop interpretation.

A Foxtail mosaic virus Vector for Virus-Induced Gene Silencing in Maize1[OPEN

Science.gov (United States)

Mei, Yu; Kernodle, Bliss M.; Hill, John H.

2016-01-01

Plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing (VIGS). A limited number of viruses have been developed into viral vectors for the purposes of gene expression or VIGS in monocotyledonous plants, and among these, the tripartite viruses Brome mosaic virus and Cucumber mosaic virus have been shown to induce VIGS in maize (Zea mays). We describe here a new DNA-based VIGS system derived from Foxtail mosaic virus (FoMV), a monopartite virus that is able to establish systemic infection and silencing of endogenous maize genes homologous to gene fragments inserted into the FoMV genome. To demonstrate VIGS applications of this FoMV vector system, four genes, phytoene desaturase (functions in carotenoid biosynthesis), lesion mimic22 (encodes a key enzyme of the porphyrin pathway), iojap (functions in plastid development), and brown midrib3 (caffeic acid O-methyltransferase), were silenced and characterized in the sweet corn line Golden × Bantam. Furthermore, we demonstrate that the FoMV infectious clone establishes systemic infection in maize inbred lines, sorghum (Sorghum bicolor), and green foxtail (Setaria viridis), indicating the potential wide applications of this viral vector system for functional genomics studies in maize and other monocots. PMID:27208311

Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM

Energy Technology Data Exchange (ETDEWEB)

McGinty, J; Requejo-Isidro, J; Munro, I; Talbot, C B; Dunsby, C; Neil, M A A; French, P M W [Photonics Group, Blackett Laboratory, Imperial College London, Prince Consort Road, London, SW7 2BW (United Kingdom); Kellett, P A; Hares, J D, E-mail: james.mcginty@imperial.ac.u [Kentech Instruments Ltd, Isis Building, Howbery Park, Wallingford, OX10 8BA (United Kingdom)

2009-07-07

Time-gated imaging using gated optical intensifiers provides a means to realize high speed fluorescence lifetime imaging (FLIM) for the study of fast events and for high throughput imaging. We present a signal-to-noise characterization of CCD-coupled micro-channel plate gated intensifiers used with this technique and determine the optimal acquisition parameters (intensifier gain voltage, CCD integration time and frame averaging) for measuring mono-exponential fluorescence lifetimes in the shortest image acquisition time for a given signal flux. We explore the use of unequal CCD integration times for different gate delays and show that this can improve the lifetime accuracy for a given total acquisition time.

A self triggered intensified Ccd (Stic)

International Nuclear Information System (INIS)

Charon, Y.; Laniece, P.; Bendali, M.

1990-01-01

We are developing a new device based on the results reported previously of the successfull coincidence detection of β- particles with a high spatial resolution [1]. The novelty of the device consists in triggering an intensified CCD, i.e. a CCD coupled to an image intensifier (II), by an electrical signal collected from the II itself. This is a suitable procedure for detecting with high efficiency and high resolution low light rare events. The trigger pulse is obtained from the secondary electrons produced by multiplication in a double microchannel plate (MCP) and collected on the aluminized layer protecting the phosphor screen in the II. Triggering efficiencies up to 80% has been already achieved

Colony Collapse Disorder (CCD) and bee age impact honey bee pathophysiology.

Science.gov (United States)

vanEngelsdorp, Dennis; Traynor, Kirsten S; Andree, Michael; Lichtenberg, Elinor M; Chen, Yanping; Saegerman, Claude; Cox-Foster, Diana L

2017-01-01

Honey bee (Apis mellifera) colonies continue to experience high annual losses that remain poorly explained. Numerous interacting factors have been linked to colony declines. Understanding the pathways linking pathophysiology with symptoms is an important step in understanding the mechanisms of disease. In this study we examined the specific pathologies associated with honey bees collected from colonies suffering from Colony Collapse Disorder (CCD) and compared these with bees collected from apparently healthy colonies. We identified a set of pathological physical characteristics that occurred at different rates in CCD diagnosed colonies prior to their collapse: rectum distension, Malpighian tubule iridescence, fecal matter consistency, rectal enteroliths (hard concretions), and venom sac color. The multiple differences in rectum symptomology in bees from CCD apiaries and colonies suggest effected bees had trouble regulating water. To ensure that pathologies we found associated with CCD were indeed pathologies and not due to normal changes in physical appearances that occur as an adult bee ages (CCD colonies are assumed to be composed mostly of young bees), we documented the changes in bees of different ages taken from healthy colonies. We found that young bees had much greater incidences of white nodules than older cohorts. Prevalent in newly-emerged bees, these white nodules or cellular encapsulations indicate an active immune response. Comparing the two sets of characteristics, we determined a subset of pathologies that reliably predict CCD status rather than bee age (fecal matter consistency, rectal distension size, rectal enteroliths and Malpighian tubule iridescence) and that may serve as biomarkers for colony health. In addition, these pathologies suggest that CCD bees are experiencing disrupted excretory physiology. Our identification of these symptoms is an important first step in understanding the physiological pathways that underlie CCD and factors

Colony Collapse Disorder (CCD and bee age impact honey bee pathophysiology.

Directory of Open Access Journals (Sweden)

Dennis vanEngelsdorp

Full Text Available Honey bee (Apis mellifera colonies continue to experience high annual losses that remain poorly explained. Numerous interacting factors have been linked to colony declines. Understanding the pathways linking pathophysiology with symptoms is an important step in understanding the mechanisms of disease. In this study we examined the specific pathologies associated with honey bees collected from colonies suffering from Colony Collapse Disorder (CCD and compared these with bees collected from apparently healthy colonies. We identified a set of pathological physical characteristics that occurred at different rates in CCD diagnosed colonies prior to their collapse: rectum distension, Malpighian tubule iridescence, fecal matter consistency, rectal enteroliths (hard concretions, and venom sac color. The multiple differences in rectum symptomology in bees from CCD apiaries and colonies suggest effected bees had trouble regulating water. To ensure that pathologies we found associated with CCD were indeed pathologies and not due to normal changes in physical appearances that occur as an adult bee ages (CCD colonies are assumed to be composed mostly of young bees, we documented the changes in bees of different ages taken from healthy colonies. We found that young bees had much greater incidences of white nodules than older cohorts. Prevalent in newly-emerged bees, these white nodules or cellular encapsulations indicate an active immune response. Comparing the two sets of characteristics, we determined a subset of pathologies that reliably predict CCD status rather than bee age (fecal matter consistency, rectal distension size, rectal enteroliths and Malpighian tubule iridescence and that may serve as biomarkers for colony health. In addition, these pathologies suggest that CCD bees are experiencing disrupted excretory physiology. Our identification of these symptoms is an important first step in understanding the physiological pathways that underlie CCD and

The Contribution of Mosaic Variants to Autism Spectrum Disorder.

Science.gov (United States)

Freed, Donald; Pevsner, Jonathan

2016-09-01

De novo mutation is highly implicated in autism spectrum disorder (ASD). However, the contribution of post-zygotic mutation to ASD is poorly characterized. We performed both exome sequencing of paired samples and analysis of de novo variants from whole-exome sequencing of 2,388 families. While we find little evidence for tissue-specific mosaic mutation, multi-tissue post-zygotic mutation (i.e. mosaicism) is frequent, with detectable mosaic variation comprising 5.4% of all de novo mutations. We identify three mosaic missense and likely-gene disrupting mutations in genes previously implicated in ASD (KMT2C, NCKAP1, and MYH10) in probands but none in siblings. We find a strong ascertainment bias for mosaic mutations in probands relative to their unaffected siblings (p = 0.003). We build a model of de novo variation incorporating mosaic variants and errors in classification of mosaic status and from this model we estimate that 33% of mosaic mutations in probands contribute to 5.1% of simplex ASD diagnoses (95% credible interval 1.3% to 8.9%). Our results indicate a contributory role for multi-tissue mosaic mutation in some individuals with an ASD diagnosis.

The Contribution of Mosaic Variants to Autism Spectrum Disorder.

Directory of Open Access Journals (Sweden)

Donald Freed

2016-09-01

Full Text Available De novo mutation is highly implicated in autism spectrum disorder (ASD. However, the contribution of post-zygotic mutation to ASD is poorly characterized. We performed both exome sequencing of paired samples and analysis of de novo variants from whole-exome sequencing of 2,388 families. While we find little evidence for tissue-specific mosaic mutation, multi-tissue post-zygotic mutation (i.e. mosaicism is frequent, with detectable mosaic variation comprising 5.4% of all de novo mutations. We identify three mosaic missense and likely-gene disrupting mutations in genes previously implicated in ASD (KMT2C, NCKAP1, and MYH10 in probands but none in siblings. We find a strong ascertainment bias for mosaic mutations in probands relative to their unaffected siblings (p = 0.003. We build a model of de novo variation incorporating mosaic variants and errors in classification of mosaic status and from this model we estimate that 33% of mosaic mutations in probands contribute to 5.1% of simplex ASD diagnoses (95% credible interval 1.3% to 8.9%. Our results indicate a contributory role for multi-tissue mosaic mutation in some individuals with an ASD diagnosis.

Comparison of a CCD and an APS for soft X-ray diffraction

International Nuclear Information System (INIS)

Stewart, Graeme; Bates, R; Blue, A; Maneuski, D; Clark, A; Turchetta, R; Dhesi, S S; Marchal, J; Steadman, P; Tartoni, N

2011-01-01

We compare a new CMOS Active Pixel Sensor (APS) to a Princeton Instruments PIXIS-XO: 2048B Charge Coupled Device (CCD) with soft X-rays tested in a synchrotron beam line at the Diamond Light Source (DLS). Despite CCDs being established in the field of scientific imaging, APS are an innovative technology that offers advantages over CCDs. These include faster readout, higher operational temperature, in-pixel electronics for advanced image processing and reduced manufacturing cost. The APS employed was the Vanilla sensor designed by the MI3 collaboration and funded by an RCUK Basic technology grant. This sensor has 520 x 520 square pixels, of size 25 μm on each side. The sensor can operate at a full frame readout of up to 20 Hz. The sensor had been back-thinned, to the epitaxial layer. This was the first time that a back-thinned APS had been demonstrated at a beam line at DLS. In the synchrotron experiment soft X-rays with an energy of approximately 708 eV were used to produce a diffraction pattern from a permalloy sample. The pattern was imaged at a range of integration times with both sensors. The CCD had to be operated at a temperature of -55°C whereas the Vanilla was operated over a temperature range from 20°C to -10°C. We show that the APS detector can operate with frame rates up to two hundred times faster than the CCD, without excessive degradation of image quality. The signal to noise of the APS is shown to be the same as that of the CCD at identical integration times and the response is shown to be linear, with no charge blooming effects. The experiment has allowed a direct comparison of back thinned APS and CCDs in a real soft x-ray synchrotron experiment.

Comparison of a CCD and an APS for soft X-ray diffraction

Science.gov (United States)

Stewart, Graeme; Bates, R.; Blue, A.; Clark, A.; Dhesi, S. S.; Maneuski, D.; Marchal, J.; Steadman, P.; Tartoni, N.; Turchetta, R.

2011-12-01

We compare a new CMOS Active Pixel Sensor (APS) to a Princeton Instruments PIXIS-XO: 2048B Charge Coupled Device (CCD) with soft X-rays tested in a synchrotron beam line at the Diamond Light Source (DLS). Despite CCDs being established in the field of scientific imaging, APS are an innovative technology that offers advantages over CCDs. These include faster readout, higher operational temperature, in-pixel electronics for advanced image processing and reduced manufacturing cost. The APS employed was the Vanilla sensor designed by the MI3 collaboration and funded by an RCUK Basic technology grant. This sensor has 520 x 520 square pixels, of size 25 μm on each side. The sensor can operate at a full frame readout of up to 20 Hz. The sensor had been back-thinned, to the epitaxial layer. This was the first time that a back-thinned APS had been demonstrated at a beam line at DLS. In the synchrotron experiment soft X-rays with an energy of approximately 708 eV were used to produce a diffraction pattern from a permalloy sample. The pattern was imaged at a range of integration times with both sensors. The CCD had to be operated at a temperature of -55°C whereas the Vanilla was operated over a temperature range from 20°C to -10°C. We show that the APS detector can operate with frame rates up to two hundred times faster than the CCD, without excessive degradation of image quality. The signal to noise of the APS is shown to be the same as that of the CCD at identical integration times and the response is shown to be linear, with no charge blooming effects. The experiment has allowed a direct comparison of back thinned APS and CCDs in a real soft x-ray synchrotron experiment.

Characterization of a new potyvirus causing mosaic and flower variegation in Catharanthus roseus in Brazil

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Sheila Conceição Maciel

2011-12-01

Full Text Available Catharanthus roseus is a perennial, evergreen herb in the family Apocynaceae, which is used as ornamental and for popular medicine to treat a wide assortment of human diseases. This paper describes a new potyvirus found causing mosaic symptom, foliar malformation and flower variegation in C. roseus. Of 28 test-plants inoculated mechanically with this potyvirus, only C. roseus and Nicotiana benthamiana developed systemic mosaic, whereas Chenopodium amaranticolor and C. quinoa exhibited chlorotic local lesions. The virus was transmitted by Aphis gossypii and Myzus nicotianae. When the nucleotide sequence of the CP gene (768nt was compared with other members of the Potyviridae family, the highest identities varied from 67 to 76 %. For the 3' UTR (286nt, identities varied from 16.8 to 28.6 %. The name Catharanthus mosaic virus (CatMV is proposed for this new potyvirus.

pnCCD for photon detection from near-infrared to X-rays

International Nuclear Information System (INIS)

Meidinger, Norbert; Andritschke, Robert; Hartmann, Robert; Herrmann, Sven; Holl, Peter; Lutz, Gerhard; Strueder, Lothar

2006-01-01

A pnCCD is a special type of charge-coupled device developed for spectroscopy and imaging of X-rays with high time resolution and quantum efficiency. Its most famous application is the operation on the XMM-Newton satellite, an X-ray astronomy mission that was launched by the European space agency in 1999. The excellent performance of the focal plane camera has been maintained for more than 6 years in orbit. The energy resolution in particular has shown hardly any degradation since launch. In order to satisfy the requirements of future X-ray astronomy missions as well as those of ground-based experiments, a new type of pnCCD has been developed. This 'frame-store pnCCD' shows an enhanced performance compared to the XMM-Newton type of pnCCD. Now, more options in device design and operation are available to tailor the detector to its respective application. Part of this concept is a programmable analog signal processor, which has been developed for the readout of the CCD signals. The electronic noise of the new detector has a value of only 2 electrons equivalent noise charge (ENC), which is less than half of the figure achieved for the XMM-Newton-type pnCCD. The energy resolution for the Mn-K α line at 5.9 keV is approximately 130 eV FWHM. We have close to 100% quantum efficiency for both low- and high-energy photon detection (e.g. the C-K line at 277 eV, and the Ge-K α line at 10 keV, respectively). Very high frame rates of 1000 images/s have been achieved due to the ultra-fast readout accomplished by the parallel architecture of the pnCCD and the analog signal processor. Excellent spectroscopic performance is shown even at the relatively high operating temperature of -25 deg. C that can be achieved by a Peltier cooler. The applications of the low-noise and fast pnCCD detector are not limited to the detection of X-rays. With an anti-reflective coating deposited on the photon entrance window, we achieve high quantum efficiency also for near-infrared and optical

Detectable clonal mosaicism and its relationship to aging and cancer

Science.gov (United States)

Jacobs, Kevin B; Yeager, Meredith; Zhou, Weiyin; Wacholder, Sholom; Wang, Zhaoming; Rodriguez-Santiago, Benjamin; Hutchinson, Amy; Deng, Xiang; Liu, Chenwei; Horner, Marie-Josephe; Cullen, Michael; Epstein, Caroline G; Burdett, Laurie; Dean, Michael C; Chatterjee, Nilanjan; Sampson, Joshua; Chung, Charles C; Kovaks, Joseph; Gapstur, Susan M; Stevens, Victoria L; Teras, Lauren T; Gaudet, Mia M; Albanes, Demetrius; Weinstein, Stephanie J; Virtamo, Jarmo; Taylor, Philip R; Freedman, Neal D; Abnet, Christian C; Goldstein, Alisa M; Hu, Nan; Yu, Kai; Yuan, Jian-Min; Liao, Linda; Ding, Ti; Qiao, You-Lin; Gao, Yu-Tang; Koh, Woon-Puay; Xiang, Yong-Bing; Tang, Ze-Zhong; Fan, Jin-Hu; Aldrich, Melinda C; Amos, Christopher; Blot, William J; Bock, Cathryn H; Gillanders, Elizabeth M; Harris, Curtis C; Haiman, Christopher A; Henderson, Brian E; Kolonel, Laurence N; Le Marchand, Loic; McNeill, Lorna H; Rybicki, Benjamin A; Schwartz, Ann G; Signorello, Lisa B; Spitz, Margaret R; Wiencke, John K; Wrensch, Margaret; Wu, Xifeng; Zanetti, Krista A; Ziegler, Regina G; Figueroa, Jonine D; Garcia-Closas, Montserrat; Malats, Nuria; Marenne, Gaelle; Prokunina-Olsson, Ludmila; Baris, Dalsu; Schwenn, Molly; Johnson, Alison; Landi, Maria Teresa; Goldin, Lynn; Consonni, Dario; Bertazzi, Pier Alberto; Rotunno, Melissa; Rajaraman, Preetha; Andersson, Ulrika; Freeman, Laura E Beane; Berg, Christine D; Buring, Julie E; Butler, Mary A; Carreon, Tania; Feychting, Maria; Ahlbom, Anders; Gaziano, J Michael; Giles, Graham G; Hallmans, Goran; Hankinson, Susan E; Hartge, Patricia; Henriksson, Roger; Inskip, Peter D; Johansen, Christoffer; Landgren, Annelie; McKean-Cowdin, Roberta; Michaud, Dominique S; Melin, Beatrice S; Peters, Ulrike; Ruder, Avima M; Sesso, Howard D; Severi, Gianluca; Shu, Xiao-Ou; Visvanathan, Kala; White, Emily; Wolk, Alicja; Zeleniuch-Jacquotte, Anne; Zheng, Wei; Silverman, Debra T; Kogevinas, Manolis; Gonzalez, Juan R; Villa, Olaya; Li, Donghui; Duell, Eric J; Risch, Harvey A; Olson, Sara H; Kooperberg, Charles; Wolpin, Brian M; Jiao, Li; Hassan, Manal; Wheeler, William; Arslan, Alan A; Bas Bueno-de-Mesquita, H; Fuchs, Charles S; Gallinger, Steven; Gross, Myron D; Holly, Elizabeth A; Klein, Alison P; LaCroix, Andrea; Mandelson, Margaret T; Petersen, Gloria; Boutron-Ruault, Marie-Christine; Bracci, Paige M; Canzian, Federico; Chang, Kenneth; Cotterchio, Michelle; Giovannucci, Edward L; Goggins, Michael; Bolton, Judith A Hoffman; Jenab, Mazda; Khaw, Kay-Tee; Krogh, Vittorio; Kurtz, Robert C; McWilliams, Robert R; Mendelsohn, Julie B; Rabe, Kari G; Riboli, Elio; Tjønneland, Anne; Tobias, Geoffrey S; Trichopoulos, Dimitrios; Elena, Joanne W; Yu, Herbert; Amundadottir, Laufey; Stolzenberg-Solomon, Rachael Z; Kraft, Peter; Schumacher, Fredrick; Stram, Daniel; Savage, Sharon A; Mirabello, Lisa; Andrulis, Irene L; Wunder, Jay S; García, Ana Patiño; Sierrasesúmaga, Luis; Barkauskas, Donald A; Gorlick, Richard G; Purdue, Mark; Chow, Wong-Ho; Moore, Lee E; Schwartz, Kendra L; Davis, Faith G; Hsing, Ann W; Berndt, Sonja I; Black, Amanda; Wentzensen, Nicolas; Brinton, Louise A; Lissowska, Jolanta; Peplonska, Beata; McGlynn, Katherine A; Cook, Michael B; Graubard, Barry I; Kratz, Christian P; Greene, Mark H; Erickson, Ralph L; Hunter, David J; Thomas, Gilles; Hoover, Robert N; Real, Francisco X; Fraumeni, Joseph F; Caporaso, Neil E; Tucker, Margaret; Rothman, Nathaniel; Pérez-Jurado, Luis A; Chanock, Stephen J

2012-01-01

In an analysis of 31,717 cancer cases and 26,136 cancer-free controls drawn from 13 genome-wide association studies (GWAS), we observed large chromosomal abnormalities in a subset of clones from DNA obtained from blood or buccal samples. Mosaic chromosomal abnormalities, either aneuploidy or copy-neutral loss of heterozygosity, of size >2 Mb were observed in autosomes of 517 individuals (0.89%) with abnormal cell proportions between 7% and 95%. In cancer-free individuals, the frequency increased with age; 0.23% under 50 and 1.91% between 75 and 79 (p=4.8×10−8). Mosaic abnormalities were more frequent in individuals with solid-tumors (0.97% versus 0.74% in cancer-free individuals, OR=1.25, p=0.016), with a stronger association for cases who had DNA collected prior to diagnosis or treatment (OR=1.45, p=0.0005). Detectable clonal mosaicism was common in individuals for whom DNA was collected at least one year prior to diagnosis of leukemia compared to cancer-free individuals (OR=35.4, p=3.8×10−11). These findings underscore the importance of the role and time-dependent nature of somatic events in the etiology of cancer and other late-onset diseases. PMID:22561519

Prenatal Diagnosis and Genetic Counseling for Mosaic Trisomy 13

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Chih-Ping Chen

2010-03-01

Full Text Available Counseling parents of a fetus with trisomy 13 mosaicism remains difficult because of the phenotypic variability associated with the condition; some patients exhibit the typical phenotype of complete trisomy 13 with neonatal death, while others have few dysmorphic features and prolonged survival. This article provides a comprehensive review of the prenatal diagnosis and genetic counseling for mosaic trisomy 13, including confined placental mosaicism 13, mosaic trisomy 13 diagnosed at amniocentesis, and phylloid hypomelanosis in association with mosaic trisomy 13.

Near-infrared Raman spectroscopy using a diode laser and CCD detector for tissue diagnostics

International Nuclear Information System (INIS)

Gustafsson, U.

1993-09-01

This paper surveys the possibility to observe high-quality NIR Raman spectra of both fluorescent and non-fluorescent samples with the use of a diode laser, a fibre optic sample, a single spectrometer and a charge-coupled device (CCD) detector. A shifted excitation difference technique was implemented for removing the broad-band fluorescence emission from Raman spectra of the highly fluorescent samples. Raman spectra of 1.4-dioxane, toluene, rhodamine 6G, and HITCI in the 640 to 1840 cm -1 spectral region and 1.4-dioxane and toluene in the 400 to 3400 cm -1 spectral region have been recorded. The results open the field of sensitive tissue characterisation and the possibility of optical biopsy in vivo by using NIR Raman spectroscopy with fibre optic sampling, a single spectrometer, and a CCD detector

A CCD-based search for very low mass members of the Pleiades cluster

Science.gov (United States)

Stauffer, John R.; Hamilton, Donald; Probst, Ronald G.

1994-01-01

We have obtained deep charge coupled device (CCD)V and I images of a number of fields near the center of the Pleiades open cluster. We have also obtained imaging data for Praesepe, a very similar cluster in terms of distance and richness but nearly 10 times older than the Pleiades. Because brown dwarfs are predicted to become much fainter and cooler between Pleiades and Praesepe ages, this provides a powerful differential technique for placing constraints on the brown dwarf population in open clusters. Combined with our previously reported observations, we now have data for about 0.4 sq deg in the Pleiades, corresponding roughly to 5% of the area of that cluster. We have searched the new CCD frames for additional Pleiades brown dwarf candidates. Two possible candidates are present, the faintest of which has V approximately equal to 22.5, (V-I)(sub K) approximately equal to 4.6. Because we do not have proper motion data and the colors of these objects are not redder than the reddest known field stars, it is possible that some or all of our candidates are somewhat higher mass field stars rather than Pleiades-age brown dwarfs. Even if all six of the proposed brown dwarf candidates in our 0.4 sq deg field are Pleiades members, the relatively small number found suggests that low mass stars or brown dwarfs do not contribute significantly to the total mass of the cluster.

Trisomy 9 Mosaicism Diagnosed In Utero

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Hironori Takahashi

2010-01-01

Full Text Available We present three cases of trisomy 9 mosaicism diagnosed by amniocentesis with ongoing pregnancies after referral to our center due to fetal abnormalities. Two cases were associated with severe fetal growth restriction (FGR, each of which resulted in an intrauterine fetal demise (IUFD in the third trimester. The other case involved mild FGR with a congenital diaphragmatic hernia and resulted in a live birth with severe development delay. A major prenatal finding of trisomy 9 mosaicism is FGR. Fetuses with trisomy 9 mosaicism can rarely survive in the case of severe FGR.

Timing generator of scientific grade CCD camera and its implementation based on FPGA technology

Science.gov (United States)

Si, Guoliang; Li, Yunfei; Guo, Yongfei

2010-10-01

The Timing Generator's functions of Scientific Grade CCD Camera is briefly presented: it generates various kinds of impulse sequence for the TDI-CCD, video processor and imaging data output, acting as the synchronous coordinator for time in the CCD imaging unit. The IL-E2TDI-CCD sensor produced by DALSA Co.Ltd. use in the Scientific Grade CCD Camera. Driving schedules of IL-E2 TDI-CCD sensor has been examined in detail, the timing generator has been designed for Scientific Grade CCD Camera. FPGA is chosen as the hardware design platform, schedule generator is described with VHDL. The designed generator has been successfully fulfilled function simulation with EDA software and fitted into XC2VP20-FF1152 (a kind of FPGA products made by XILINX). The experiments indicate that the new method improves the integrated level of the system. The Scientific Grade CCD camera system's high reliability, stability and low power supply are achieved. At the same time, the period of design and experiment is sharply shorted.

Ploidy mosaicism and allele-specific gene expression differences in the allopolyploid Squalius alburnoides

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Matos Isa

2011-12-01

Full Text Available Abstract Background Squalius alburnoides is an Iberian cyprinid fish resulting from an interspecific hybridisation between Squalius pyrenaicus females (P genome and males of an unknown Anaecypris hispanica-like species (A genome. S. alburnoides is an allopolyploid hybridogenetic complex, which makes it a likely candidate for ploidy mosaicism occurrence, and is also an interesting model to address questions about gene expression regulation and genomic interactions. Indeed, it was previously suggested that in S. alburnoides triploids (PAA composition silencing of one of the three alleles (mainly of the P allele occurs. However, not a whole haplome is inactivated but a more or less random inactivation of alleles varying between individuals and even between organs of the same fish was seen. In this work we intended to correlate expression differences between individuals and/or between organs to the occurrence of mosaicism, evaluating if mosaics could explain previous observations and its impact on the assessment of gene expression patterns. Results To achieve our goal, we developed flow cytometry and cell sorting protocols for this system generating more homogenous cellular and transcriptional samples. With this set-up we detected 10% ploidy mosaicism within the S. alburnoides complex, and determined the allelic expression profiles of ubiquitously expressed genes (rpl8; gapdh and β-actin in cells from liver and kidney of mosaic and non-mosaic individuals coming from different rivers over a wide geographic range. Conclusions Ploidy mosaicism occurs sporadically within the S. alburnoides complex, but in a frequency significantly higher than reported for other organisms. Moreover, we could exclude the influence of this phenomenon on the detection of variable allelic expression profiles of ubiquitously expressed genes (rpl8; gapdh and β-actin in cells from liver and kidney of triploid individuals. Finally, we determined that the expression patterns

The LOFT wide field monitor simulator

DEFF Research Database (Denmark)

Donnarumma, I.; Evangelista, Y.; Campana, R.

2012-01-01

We present the simulator we developed for the Wide Field Monitor (WFM) aboard the Large Observatory For Xray Timing (LOFT) mission, one of the four ESA M3 candidate missions considered for launch in the 2022–2024 timeframe. The WFM is designed to cover a large FoV in the same bandpass as the Large...

CCD characterization and measurements automation

Czech Academy of Sciences Publication Activity Database

Kotov, I.V.; Frank, J.; Kotov, A.I.; Kubánek, Petr; O´Connor, P.; Prouza, Michael; Radeka, V.; Takacs, P.

2012-01-01

Roč. 695, Dec (2012), 188-192 ISSN 0168-9002 R&D Projects: GA MŠk ME09052 Institutional research plan: CEZ:AV0Z10100502 Keywords : CCD * characterization * test automation Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 1.142, year: 2012

Confirmation of the reported association of clonal chromosomal mosaicism with an increased risk of incident hematologic cancer.

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Ursula M Schick

Full Text Available Chromosomal abnormalities provide clinical utility in the diagnosis and treatment of hematologic malignancies, and may be predictive of malignant transformation in individuals without apparent clinical presentation of a hematologic cancer. In an effort to confirm previous reports of an association between clonal mosaicism and incident hematologic cancer, we applied the anomDetectBAF algorithm to call chromosomal anomalies in genotype data from previously conducted Genome Wide Association Studies (GWAS. The genotypes were initially collected from DNA derived from peripheral blood of 12,176 participants in the Group Health electronic Medical Records and Genomics study (eMERGE and the Women's Health Initiative (WHI. We detected clonal mosaicism in 169 individuals (1.4% and large clonal mosaic events (>2 mb in 117 (1.0% individuals. Though only 9.5% of clonal mosaic carriers had an incident diagnosis of hematologic cancer (multiple myeloma, myelodysplastic syndrome, lymphoma, or leukemia, the carriers had a 5.5-fold increased risk (95% CI: 3.3-9.3; p-value = 7.5×10(-11 of developing these cancers subsequently. Carriers of large mosaic anomalies showed particularly pronounced risk of subsequent leukemia (HR = 19.2, 95% CI: 8.9-41.6; p-value = 7.3×10(-14. Thus we independently confirm the association between detectable clonal mosaicism and hematologic cancer found previously in two recent publications.

Design and Development of Multi-Purpose CCD Camera System with Thermoelectric Cooling: Hardware

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Y.-W. Kang

2007-12-01

Full Text Available We designed and developed a multi-purpose CCD camera system for three kinds of CCDs; KAF-0401E(768×512, KAF-1602E(1536×1024, KAF-3200E(2184×1472 made by KODAK Co.. The system supports fast USB port as well as parallel port for data I/O and control signal. The packing is based on two stage circuit boards for size reduction and contains built-in filter wheel. Basic hardware components include clock pattern circuit, A/D conversion circuit, CCD data flow control circuit, and CCD temperature control unit. The CCD temperature can be controlled with accuracy of approximately 0.4° C in the max. range of temperature, Δ 33° C. This CCD camera system has with readout noise 6 e^{-}, and system gain 5 e^{-}/ADU. A total of 10 CCD camera systems were produced and our tests show that all of them show passable performance.

Health Profiles of Mosaic Versus Non-mosaic FMR1 Premutation Carrier Mothers of Children With Fragile X Syndrome

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Marsha R. Mailick

2018-05-01

Full Text Available The FMR1 premutation is of increasing interest to the FXS community, as questions about a primary premutation phenotype warrant research attention. 100 FMR1 premutation carrier mothers (mean age = 58; 67–138 CGG repeats of adults with fragile X syndrome were studied with respect to their physical and mental health, motor, and neurocognitive characteristics. We explored the correlates of CGG repeat mosaicism in women with expanded alleles. Mothers provided buccal swabs from which DNA was extracted and the FMR1 CGG genotyping was performed (Amplidex Kit, Asuragen. Mothers were categorized into three groups: Group 1: premutation non-mosaic (n = 45; Group 2: premutation mosaic (n = 41, and Group 3: premutation/full mutation mosaic (n = 14. Group 2 mothers had at least two populations of cells with different allele sizes in the premutation range besides their major expanded allele. Group 3 mothers had a very small population of cells in the full mutation range (>200 CGGs in addition to one or multiple populations of cells with different allele sizes in the premutation range. Machine learning (random forest was used to identify symptoms and conditions that correctly classified mothers with respect to mosaicism; follow-up comparisons were made to characterize the three groups. In categorizing mosaicism, the random forest yielded significantly better classification than random classification, with overall area under the receiver operating characteristic curve (AUROC of 0.737. Among the most important symptoms and conditions that contributed to the classification were anxiety, menopause symptoms, executive functioning limitations, and difficulty walking several blocks, with the women who had full mutation mosaicism (Group 3 unexpectedly having better health. Although only 14 premutation carrier mothers in the present sample also had a small population of full mutation cells, their profile of comparatively better health, mental health, and executive

CANDELS: THE COSMIC ASSEMBLY NEAR-INFRARED DEEP EXTRAGALACTIC LEGACY SURVEY—THE HUBBLE SPACE TELESCOPE OBSERVATIONS, IMAGING DATA PRODUCTS, AND MOSAICS

International Nuclear Information System (INIS)

Koekemoer, Anton M.; Ferguson, Henry C.; Grogin, Norman A.; Lotz, Jennifer M.; Lucas, Ray A.; Ogaz, Sara; Rajan, Abhijith; Casertano, Stefano; Dahlen, Tomas; Faber, S. M.; Kocevski, Dale D.; Koo, David C.; Lai, Kamson; McGrath, Elizabeth J.; Riess, Adam G.; Rodney, Steve A.; Dolch, Timothy; Strolger, Louis; Castellano, Marco; Dickinson, Mark

2011-01-01

This paper describes the Hubble Space Telescope imaging data products and data reduction procedures for the Cosmic Assembly Near-infrared Deep Extragalactic Legacy Survey (CANDELS). This survey is designed to document the evolution of galaxies and black holes at z ≈ 1.5-8, and to study Type Ia supernovae at z > 1.5. Five premier multi-wavelength sky regions are selected, each with extensive multi-wavelength observations. The primary CANDELS data consist of imaging obtained in the Wide Field Camera 3 infrared channel (WFC3/IR) and the WFC3 ultraviolet/optical channel, along with the Advanced Camera for Surveys (ACS). The CANDELS/Deep survey covers ∼125 arcmin 2 within GOODS-N and GOODS-S, while the remainder consists of the CANDELS/Wide survey, achieving a total of ∼800 arcmin 2 across GOODS and three additional fields (Extended Groth Strip, COSMOS, and Ultra-Deep Survey). We summarize the observational aspects of the survey as motivated by the scientific goals and present a detailed description of the data reduction procedures and products from the survey. Our data reduction methods utilize the most up-to-date calibration files and image combination procedures. We have paid special attention to correcting a range of instrumental effects, including charge transfer efficiency degradation for ACS, removal of electronic bias-striping present in ACS data after Servicing Mission 4, and persistence effects and other artifacts in WFC3/IR. For each field, we release mosaics for individual epochs and eventual mosaics containing data from all epochs combined, to facilitate photometric variability studies and the deepest possible photometry. A more detailed overview of the science goals and observational design of the survey are presented in a companion paper.

Chromosome mosaicism in hypomelanosis of Ito.

Science.gov (United States)

Ritter, C L; Steele, M W; Wenger, S L; Cohen, B A

1990-01-01

Our finding of chromosome mosaicism with a ring 22 in a retarded black boy with hypomelanosis of Ito prompted a review of this "syndrome." Most patients have a variety of non-dermal defects, particularly those affecting CNS function. Among karyotyped patients, most are chromosome mosaics of one sort or another. Hypomelanosis of Ito turns out to be a causable non-specific phenotype, i.e., a clinical marker for chromosome mosaicism of all different types in individuals with a dark enough skin to show lighter patches. Consequently, cytogenetic evaluation is indicated in all patients with this skin finding.

Kisaran Inang dan Keragaman Gejala Infeksi Turnip Mosaic Virus

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Eliza Suryati Rusli

2007-07-01

Full Text Available The incidence of mosaic disease on vegetable crops in Indonesia has been reported recently. The disease is caused by TuMV which is considered as a new and important virus on caisin and turnip in Indonesia. Field survey has been conducted to determine disease incidence in vegetable growing areas. Symptom variability and host range of TuMV was further studied through mechanical inoculation to cruciferae and solanaceae plants. Observation during field survey has proved that TuMV has infected caisin and turnip in Java and Bali. The highest intensity of mosaic disease i.e. 63,3% occurs in Tumpangan-Malang, followed by Denpasar Selatan and Bandungan-Semarang with the intensity of 30,5% and 19,0% respectively. TuMV infection causes different types of symptoms, such as: wrinkled leaf, blistered leaf, vein banding, vein clearing, leaf distortion and proliferation. The host range of TuMV involves those plants belong to cruciferae (cabbage, broccoli, caisin, turnip, cauliflower, chinese cabbage, pak coy; solanaceae (N. tabacum, N. benthamiana, N. glutinosa; and chenopodiaceae (C. amaranticolor. Furthermore, N. glutinosa can be used as differential host for TuMV isolates.

Experimental investigations of image quality in X-ray mammography with conventional screen film system (SFS), digital phosphor storage plate in/without magnification technique (CR) and digital CCD-technique (CCD)

International Nuclear Information System (INIS)

Schulz-Wendtland, R.; Aichinger, U.; Boehner, C.; Dobritz, M.; Wenkel, E.; Bautz, W.; Saebel, M.

2001-01-01

Comparison of image quality in X-ray mammography between conventional film screen film system (SFS), digital phosphor storage plate in and without magnification technique (CR) and digital CCD-technique (CCD). Radiograms of an RMI-mammography phantom were acquired using a conventional screen film system, three digital storage plate systems and two digital systems in CCD-technique. Additionally the radiograms of one digital phosphor storage plate system were post-processed regarding contrast and included in the comparison. The detectability of details was best with the digital mammography in CCD-technique. After confirming these promising results in clinical studies, digital mammography should be able to replace conventional screen film technique. (orig.)

Modeling the impact of preflushing on CTE in proton irradiated CCD-based detectors

Science.gov (United States)

Philbrick, R. H.

2002-04-01

A software model is described that performs a "real world" simulation of the operation of several types of charge-coupled device (CCD)-based detectors in order to accurately predict the impact that high-energy proton radiation has on image distortion and modulation transfer function (MTF). The model was written primarily to predict the effectiveness of vertical preflushing on the custom full frame CCD-based detectors intended for use on the proposed Kepler Discovery mission, but it is capable of simulating many other types of CCD detectors and operating modes as well. The model keeps track of the occupancy of all phosphorous-silicon (P-V), divacancy (V-V) and oxygen-silicon (O-V) defect centers under every CCD electrode over the entire detector area. The integrated image is read out by simulating every electrode-to-electrode charge transfer in both the vertical and horizontal CCD registers. A signal level dependency on the capture and emission of signal is included and the current state of each electrode (e.g., barrier or storage) is considered when distributing integrated and emitted signal. Options for performing preflushing, preflashing, and including mini-channels are available on both the vertical and horizontal CCD registers. In addition, dark signal generation and image transfer smear can be selectively enabled or disabled. A comparison of the charge transfer efficiency (CTE) data measured on the Hubble space telescope imaging spectrometer (STIS) CCD with the CTE extracted from model simulations of the STIS CCD show good agreement.

Deflection control system for prestressed concrete bridges by CCD camera. CCD camera ni yoru prestressed concrete kyo no tawami kanri system

Energy Technology Data Exchange (ETDEWEB)

Noda, Y.; Nakayama, Y.; Arai, T. (Kawada Construction Co. Ltd., Tokyo (Japan))

1994-03-15

For the long-span prestressed concrete bridge (continuous box girder and cable stayed bridge), the design and construction control becomes increasingly complicated as construction proceeds because of its cyclic works. This paper describes the method and operation of an automatic levelling module using CCD camera and the experimental results by this system. For this automatic levelling system, the altitude can be automatically measured by measuring the center location of gravity of the target on the bridge surface using CCD camera. The present deflection control system developed compares the measured value by the automatic levelling system with the design value obtained by the design calculation system, and manages them. From the real-time continuous measurement for the long term, in which the CCD camera was set on the bridge surface, it was found that the stable measurement accuracy can be obtained. Successful application of this system demonstrates that the system is an effective and efficient construction aid. 11 refs., 19 figs., 1 tab.

One method for HJ-1-A HSI and CCD data fusion

International Nuclear Information System (INIS)

Xiong, Wencheng; Shao, Yun; Shen, Wenming; Xiao, Rulin; Fu, Zhuo; Shi, Yuanli

2014-01-01

HJ-1-A satellite, developed by China independently, was equipped with two sensors of Hyper Spectral Imager (HSI) and multispectral sensor (CCD). In this paper, we examine the benefits of combining data from CCD data (high-spatial-resolution, low-spectral-resolution image) with HSI data (low -spatial-resolution, high -spectral-resolution image). Due to the same imaging time and similar spectral regime, the CCD and HSI data can be registered with each other well, and the difference between CCD and HSI data mainly is systematic bias. The approach we have been investigating compares the spectral information present in the multispectral image to the spectral content in the hyperspectral image, and derives a set of equations to approximately acquire the systematic bias between the two sensors. The systematic bias is then applied to the interpolated high-spectral CCD image to produce a fused product. This fused image has the spectral resolution of the hyperspectral image (HSI) and the spatial resolution of the multispectral image (CCD). It is capable of full exploitation as a hyperspectral image. We evaluate this technique using the data of Honghe wetland and show both good spectral and visual fidelity. An analysis of SAM classification test case shows good result when compared to original image. All in all, the approach we developed here provides a means for fusing data from HJ-1-A satellite to produce a spatial-resolution-enhanced hyperspectral data cube that can be further analyzed by spectral classification and detection algorithms

MONOCLONAL ANTIBODIES TO IDENTIFY TOMATO MOSAIC TOBAMOVIRUS (TOMV

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Duarte Keila M.R.

2001-01-01

Full Text Available Monoclonal antibodies were obtained against Tomato mosaic tobamovirus (ToMV isolated in Brazil. One antibody (8G7G2 isotyped as IgG2b (kappa light chain showed strong specificity and very low cross reaction with the Tobacco mosaic virus (TMV. It can be used in identification of tomato mosaic virus (ToMV.

Comparison of a CCD and an APS for soft x-ray diffraction

OpenAIRE

Stewart, G.; Bates, R.; Blue, A.; Clark, A.; Dhesi, S.S.; Maneuski, D.; Marchal, J.; Steadman, P.; Tartoni, N.; Turchetta, R.

2011-01-01

We compare a new CMOS Active Pixel Sensor (APS) to a Princeton Instruments PIXIS-XO: 2048B Charge Coupled Device (CCD) with soft X-rays tested in a synchrotron beam line at the Diamond Light Source (DLS). Despite CCDs being established in the field of scientific imaging, APS are an innovative technology that offers advantages over CCDs. These include faster readout, higher operational temperature, in-pixel electronics for advanced image processing and reduced manufacturing cost.\\ud \\ud The AP...

Sex mosaics in a male dimorphic ant Cardiocondyla kagutsuchi

Science.gov (United States)

Yoshizawa, Juri; Mimori, Kohei; Yamauchi, Katsusuke; Tsuchida, Koji

2009-01-01

Gynandromorphy, or the development of organisms with a combination of male and female morphological features, is common in Hymenoptera. The underlying mechanism is likely associated with the sex-determination system, and studying this phenomenon should lead to a deeper understanding of both embryonic development and sex determination. The reproductive capabilities of gynandromorphs (hereafter, sex mosaics) remain unclear. We studied gynandromorphy in the Malaysian ant Cardiocondyla kagutsuchi, which has sex mosaics of queens (gynandromorphs; mosaic of queens and winged male) and workers (ergatandromorphs; mosaic of worker and wingless ergatoid male). These sex mosaics were classified into seven morphological categories. Most individuals had more male than female body areas. Behavioral observations revealed that sex mosaics behave more in accordance with the “sex” of their brain than that of the reproductive organs (gaster). Relative DNA quantities showed that both female and male regions contained haploid and diploid nuclei, irrespective of their phenotypic appearance, indicating that external appearance did not reflect internal tissues. Nearly one third of the adults were sex mosaics and they were not infected with Wolbachia. Our results suggest that the production of sex mosaics in this species does not pose a substantial cost to colonies and that the underlying causes are therefore not strongly selected against.

A model for measurement of noise in CCD digital-video cameras

International Nuclear Information System (INIS)

Irie, K; Woodhead, I M; McKinnon, A E; Unsworth, K

2008-01-01

This study presents a comprehensive measurement of CCD digital-video camera noise. Knowledge of noise detail within images or video streams allows for the development of more sophisticated algorithms for separating true image content from the noise generated in an image sensor. The robustness and performance of an image-processing algorithm is fundamentally limited by sensor noise. The individual noise sources present in CCD sensors are well understood, but there has been little literature on the development of a complete noise model for CCD digital-video cameras, incorporating the effects of quantization and demosaicing

Frequency and Molecular Characterization of Watermelon Mosaic Virus from Serbia

Directory of Open Access Journals (Sweden)

Ana Vučurović

2010-01-01

Full Text Available Watermelon mosaic virus (WMV is widespread in cucurbit crops, most commonly occuring in temperate and Mediterranean regions. In Serbia WMV has been detected in single and mixed infections with Zucchini yellow mosaic virus and Cucumber mosaic virus in field-grown pumpkin and squash crops. Among pumpkin-affecting viruses WMV is the most frequent one, both by the number of localities and its incidence at each location. During the growing season of 2009, samples from 583 plants of Cucurbita pepo cvs. Olinka, Belgrade zucchini and Tosca (Zucchini group, as well as from C. maxima and C. moschata showing symptoms of virus infection were collected from 12 commercial fields at eight localities and analyzed by DAS-ELISA using polyclonal antisera specific to six most important cucurbit viruses. Interestingly, WMV was detected at fewer sites and had lower ncidence rate than in two previous years. In single infections, WMV was found in 11% of tested plants in three fields; in mixed infections with ZYMV, it was recorded in 9.9% of plants in five fields and with CMV in only 0.2% in one field. The partial coat protein gene and 3’ non-translated region from two representativeisolates of WMV originating from different localities and host plant species were amplified by RT-PCR, sequenced, and compared with the sequences available in GenBank database. The PCR-amplified fragment of predicted size of approximately 1017 bp was obtained. The sequences of isolates 137-08 (Acc. No. GQ259958 and 159-08 (GU144020 proved to be 94-99% identical at the nucleotide level with those from other parts of the world. The sequences of these two isolates differed from each other only at two nucleotide positions, without any amino acid substitution. Phylogenetic analysis of 57 isolates based on 750 bp sequences of the coat protein gene showed no correlation between isolates and their geographic origin, and italso indicated that these isolates fell into three molecular groups of

Noise analysis for CCD-based ultraviolet and visible spectrophotometry.

Science.gov (United States)

Davenport, John J; Hodgkinson, Jane; Saffell, John R; Tatam, Ralph P

2015-09-20

We present the results of a detailed analysis of the noise behavior of two CCD spectrometers in common use, an AvaSpec-3648 CCD UV spectrometer and an Ocean Optics S2000 Vis spectrometer. Light sources used include a deuterium UV/Vis lamp and UV and visible LEDs. Common noise phenomena include source fluctuation noise, photoresponse nonuniformity, dark current noise, fixed pattern noise, and read noise. These were identified and characterized by varying light source, spectrometer settings, or temperature. A number of noise-limiting techniques are proposed, demonstrating a best-case spectroscopic noise equivalent absorbance of 3.5×10(-4)  AU for the AvaSpec-3648 and 5.6×10(-4)  AU for the Ocean Optics S2000 over a 30 s integration period. These techniques can be used on other CCD spectrometers to optimize performance.

Investigation of mosaicity of epitaxic multilayers by the statistical theory of X-ray dynamical diffraction

International Nuclear Information System (INIS)

Li Ming; Mai Zhenhong; Li Jianhua; Li Chaorong; Cui Shufan

1995-01-01

Based on the statistical theory of X-ray dynamical diffraction for thin films, the mosaicity of three types of semiconductor epitaxic layers has been investigated by analyzing their rocking curves by the X-ray double-crystal diffraction method. It is shown that the statistical theory can provide quantitative information on the mosaicity of the epitaxic layers such as the mean size and the mean disorientation of mosaic blocks in the layers. Some misunderstandings in interpreting experimental data are cleared up by taking into account the effect of diffuse scattering. It is emphasized that attempts to obtain structural parameters of specimens from their rocking curves by means of the Takagi-Taupin equations for coherent fields only are not strictly correct since diffuse scattering causes additional changes in the tails of the rocking curves. (orig.)

Laser Light-field Fusion for Wide-field Lensfree On-chip Phase Contrast Microscopy of Nanoparticles

Science.gov (United States)

Kazemzadeh, Farnoud; Wong, Alexander

2016-12-01

Wide-field lensfree on-chip microscopy, which leverages holography principles to capture interferometric light-field encodings without lenses, is an emerging imaging modality with widespread interest given the large field-of-view compared to lens-based techniques. In this study, we introduce the idea of laser light-field fusion for lensfree on-chip phase contrast microscopy for detecting nanoparticles, where interferometric laser light-field encodings acquired using a lensfree, on-chip setup with laser pulsations at different wavelengths are fused to produce marker-free phase contrast images of particles at the nanometer scale. As a proof of concept, we demonstrate, for the first time, a wide-field lensfree on-chip instrument successfully detecting 300 nm particles across a large field-of-view of ~30 mm2 without any specialized or intricate sample preparation, or the use of synthetic aperture- or shift-based techniques.

Cucurbits depicted in Byzantine mosaics from Israel, 350-600 ce.

Science.gov (United States)

Avital, Anat; Paris, Harry S

2014-08-01

Thousands of floor mosaics were produced in lands across the Roman and Byzantine empires. Some mosaics contain depictions of agricultural produce, potentially providing useful information concerning the contemporary presence and popularity of crop plants in a particular geographical region. Hundreds of floor mosaics produced in Israel during the Byzantine period have survived. The objective of the present work was to search these mosaics for Cucurbitaceae in order to obtain a more complete picture of cucurbit crop history in the eastern Mediterranean region. Twenty-three mosaics dating from 350-600 ce were found that had images positively identifiable as cucurbits. The morphological diversity of the cucurbit fruits in the mosaics of Israel is greater than that appearing in mosaics from any other Roman or Byzantine provincial area. The depicted fruits vary in shape from oblate to extremely long, and some are furrowed, others are striped and others lack definite markings. The cucurbit taxa depicted in the mosaics are Cucumis melo (melon), Citrullus lanatus (watermelon), Luffa aegyptiaca (sponge gourd) and Lagenaria siceraria (bottle gourd). Cucumis melo is the most frequently found taxon in the mosaics and is represented by round dessert melons and long snake melons. Fruits of at least two cultivars of snake melons and of watermelons are represented. To our knowledge, images of sponge gourds have not been found in Roman and Byzantine mosaics elsewhere. Indeed, the mosaics of Israel contain what are probably the oldest depictions of Luffa aegyptiaca in Mediterranean lands. Sponge gourds are depicted often, in 11 of the mosaics at eight localities, and the images include both mature fruits, which are useful for cleaning and washing, and immature fruits, which are edible. Only one mosaic has images positively identifiable as of bottle gourds, and these were round-pyriform and probably used as vessels. © The Author 2014. Published by Oxford University Press on behalf of

Behavioral Variability and Somatic Mosaicism: A Cytogenomic Hypothesis.

Science.gov (United States)

Vorsanova, Svetlana G; Zelenova, Maria A; Yurov, Yuri B; Iourov, Ivan Y

2018-04-01

Behavioral sciences are inseparably related to genetics. A variety of neurobehavioral phenotypes are suggested to result from genomic variations. However, the contribution of genetic factors to common behavioral disorders (i.e. autism, schizophrenia, intellectual disability) remains to be understood when an attempt to link behavioral variability to a specific genomic change is made. Probably, the least appreciated genetic mechanism of debilitating neurobehavioral disorders is somatic mosaicism or the occurrence of genetically diverse (neuronal) cells in an individual's brain. Somatic mosaicism is assumed to affect directly the brain being associated with specific behavioral patterns. As shown in studies of chromosome abnormalities (syndromes), genetic mosaicism is able to change dynamically the phenotype due to inconsistency of abnormal cell proportions. Here, we hypothesize that brain-specific postzygotic changes of mosaicism levels are able to modulate variability of behavioral phenotypes. More precisely, behavioral phenotype variability in individuals exhibiting somatic mosaicism might correlate with changes in the amount of genetically abnormal cells throughout the lifespan. If proven, the hypothesis can be used as a basis for therapeutic interventions through regulating levels of somatic mosaicism to increase functioning and to improve overall condition of individuals with behavioral problems.

Radiation damage of the PCO Pixelfly VGA CCD camera of the BES system on KSTAR tokamak

Energy Technology Data Exchange (ETDEWEB)

Náfrádi, Gábor, E-mail: nafradi@reak.bme.hu [NTI, BME, EURATOM Association, H-1111 Budapest (Hungary); Kovácsik, Ákos, E-mail: kovacsik.akos@reak.bme.hu [NTI, BME, EURATOM Association, H-1111 Budapest (Hungary); Pór, Gábor, E-mail: por@reak.bme.hu [NTI, BME, EURATOM Association, H-1111 Budapest (Hungary); Lampert, Máté, E-mail: lampert.mate@wigner.mta.hu [Wigner RCP, RMI, EURATOM Association, POB 49, 1525 Budapest (Hungary); Un Nam, Yong, E-mail: yunam@nfri.re.kr [NFRI, 169-148 Gwahak-Ro, Yuseong-Gu, Daejeon 305-806 (Korea, Republic of); Zoletnik, Sándor, E-mail: zoletnik.sandor@wigner.mta.hu [Wigner RCP, RMI, EURATOM Association, POB 49, 1525 Budapest (Hungary)

2015-01-11

A PCO Pixelfly VGA CCD camera which is part a of the Beam Emission Spectroscopy (BES) diagnostic system of the Korea Superconducting Tokamak Advanced Research (KSTAR) used for spatial calibrations, suffered from serious radiation damage, white pixel defects have been generated in it. The main goal of this work was to identify the origin of the radiation damage and to give solutions to avoid it. Monte Carlo N-Particle eXtended (MCNPX) model was built using Monte Carlo Modeling Interface Program (MCAM) and calculations were carried out to predict the neutron and gamma-ray fields in the camera position. Besides the MCNPX calculations pure gamma-ray irradiations of the CCD camera were carried out in the Training Reactor of BME. Before, during and after the irradiations numerous frames were taken with the camera with 5 s long exposure times. The evaluation of these frames showed that with the applied high gamma-ray dose (1.7 Gy) and dose rate levels (up to 2 Gy/h) the number of the white pixels did not increase. We have found that the origin of the white pixel generation was the neutron-induced thermal hopping of the electrons which means that in the future only neutron shielding is necessary around the CCD camera. Another solution could be to replace the CCD camera with a more radiation tolerant one for example with a suitable CMOS camera or apply both solutions simultaneously.

The X-ray mirror telescope and the pn-CCD detector of CAST

CERN Document Server

Kuster, M; Englhauser, J; Franz, J; Friedrich, P; Hartmann, R; Kang, D; Kotthaus, R; Lutz, Gerhard; Moralez, J; Serber, W; Strüder, L

2004-01-01

The Cern Axion Solar Telescope - CAST - uses a prototype 9 Tesla LHC superconducting dipole magnet to search for a hypothetical pseudoscalar particle, the axion, which was proposed by theory in the 1980s to solve the strong CP problem and which could be a dark matter candidate. In CAST a strong magnetic field is used to convert the solar axions to detectable photons via inverse Primakoff effect. The resulting X-rays are thermally distributed in the energy range of 1-7 keV and can be observed with conventional X-ray detectors. The most sensitive detector system of CAST is a pn-CCD detector originally developed for XMM-Newton combined with a Wolter I type X-ray mirror system. The combination of a focusing X-ray optics and a state of the art pn-CCD detector which combines high quantum efficiency, good spacial and energy resolution, and low background improves the sensitivity of the CAST experiment such that for the first time the axion photon coupling constant can be probed beyond the best astrophysical constrai...

A Bridge Deflection Monitoring System Based on CCD

Directory of Open Access Journals (Sweden)

Baohua Shan

2016-01-01

Full Text Available For long-term monitoring of the midspan deflection of Songjiazhuang cloverleaf junction on 309 national roads in Zibo city, this paper proposes Zhang’s calibration-based DIC deflection monitoring method. CCD cameras are used to track the change of targets’ position, Zhang’s calibration algorithm is introduced to acquire the intrinsic and extrinsic parameters of CCD cameras, and the DIC method is combined with Zhang’s calibration algorithm to measure bridge deflection. The comparative test between Zhang’s calibration and scale calibration is conducted in lab, and experimental results indicate that the proposed method has higher precision. According to the deflection monitoring scheme, the deflection monitoring software for Songjiazhuang cloverleaf junction is developed by MATLAB, and a 4-channel CCD deflection monitoring system for Songjiazhuang cloverleaf junction is integrated in this paper. This deflection monitoring system includes functions such as image preview, simultaneous collection, camera calibration, deflection display, and data storage. In situ deflection curves show a consistent trend; this suggests that the proposed method is reliable and is suitable for the long-term monitoring of bridge deflection.

Development of field-wide risk based remediation objectives for an aging oil field : Devon Canada Swan Hills Field

Energy Technology Data Exchange (ETDEWEB)

Brewster, M.; North, C.; Leighton-Boyce, G. [WorleyParsons Komex, Calgary, AB (Canada); Moore, D. [Devon Canada Corp., Calgary, AB (Canada)

2006-07-01

The development of field-wide risk based remediation objectives for the aging Devon Canada Swan Hills oil field was examined along with the key components of the closure strategy. These included source removal to the extent practical, long term monitoring, and achievable risk-based remedial objectives that were appropriate to the remote boreal forest setting of the Swan Hills field. A two stage approach was presented. The first stage involved a field wide background framework which included defining areas of common physical and ecological setting and developing appropriate exposure scenarios. The second stage involved site-specific risk assessments which included adjusting for site-specific conditions and an early demonstration project to prove the concept. A GIS approach was used to identify areas of common physical and ecological setting including: physiography; surface water; land use; vegetation ecozones; surficial and bedrock geology; and water well use. Species lists were compiled for vegetation, terrestrial wildlife (mammals, birds, amphibians), and aquatic species (fish and invertebrates). Major contaminant sources, problem formulation, vegetation bioassays, invertebrate bioassays, black spruce emergence, and guideline development were other topics covered during the presentation. Last, a summary of progress was presented. A field-wide review and development of risk zones and site-specific risk assessment has been completed. A regulatory review is underway. tabs., figs.

Developing a CCD camera with high spatial resolution for RIXS in the soft X-ray range

Science.gov (United States)

Soman, M. R.; Hall, D. J.; Tutt, J. H.; Murray, N. J.; Holland, A. D.; Schmitt, T.; Raabe, J.; Schmitt, B.

2013-12-01

The Super Advanced X-ray Emission Spectrometer (SAXES) at the Swiss Light Source contains a high resolution Charge-Coupled Device (CCD) camera used for Resonant Inelastic X-ray Scattering (RIXS). Using the current CCD-based camera system, the energy-dispersive spectrometer has an energy resolution (E/ΔE) of approximately 12,000 at 930 eV. A recent study predicted that through an upgrade to the grating and camera system, the energy resolution could be improved by a factor of 2. In order to achieve this goal in the spectral domain, the spatial resolution of the CCD must be improved to better than 5 μm from the current 24 μm spatial resolution (FWHM). The 400 eV-1600 eV energy X-rays detected by this spectrometer primarily interact within the field free region of the CCD, producing electron clouds which will diffuse isotropically until they reach the depleted region and buried channel. This diffusion of the charge leads to events which are split across several pixels. Through the analysis of the charge distribution across the pixels, various centroiding techniques can be used to pinpoint the spatial location of the X-ray interaction to the sub-pixel level, greatly improving the spatial resolution achieved. Using the PolLux soft X-ray microspectroscopy endstation at the Swiss Light Source, a beam of X-rays of energies from 200 eV to 1400 eV can be focused down to a spot size of approximately 20 nm. Scanning this spot across the 16 μm square pixels allows the sub-pixel response to be investigated. Previous work has demonstrated the potential improvement in spatial resolution achievable by centroiding events in a standard CCD. An Electron-Multiplying CCD (EM-CCD) has been used to improve the signal to effective readout noise ratio achieved resulting in a worst-case spatial resolution measurement of 4.5±0.2 μm and 3.9±0.1 μm at 530 eV and 680 eV respectively. A method is described that allows the contribution of the X-ray spot size to be deconvolved from these

Single-Electron and Single-Photon Sensitivity with a Silicon Skipper CCD

Science.gov (United States)

Tiffenberg, Javier; Sofo-Haro, Miguel; Drlica-Wagner, Alex; Essig, Rouven; Guardincerri, Yann; Holland, Steve; Volansky, Tomer; Yu, Tien-Tien

2017-09-01

We have developed ultralow-noise electronics in combination with repetitive, nondestructive readout of a thick, fully depleted charge-coupled device (CCD) to achieve an unprecedented noise level of 0.068 e- rms /pixel . This is the first time that discrete subelectron readout noise has been achieved reproducible over millions of pixels on a stable, large-area detector. This enables the contemporaneous, discrete, and quantized measurement of charge in pixels, irrespective of whether they contain zero electrons or thousands of electrons. Thus, the resulting CCD detector is an ultra-sensitive calorimeter. It is also capable of counting single photons in the optical and near-infrared regime. Implementing this innovative non-destructive readout system has a negligible impact on CCD design and fabrication, and there are nearly immediate scientific applications. As a particle detector, this CCD will have unprecedented sensitivity to low-mass dark matter particles and coherent neutrino-nucleus scattering, while future astronomical applications may include direct imaging and spectroscopy of exoplanets.

Deep CCD photometry in globular clusters III. M15

International Nuclear Information System (INIS)

Fahlman, G.G.; Richer, H.B.; Vandenberg, D.A.

1985-01-01

CCD photometry in U, B, and V is presented for a 5' x 3' field in the globular cluster M15. The location of the main sequence in the color-magnitude diagram is found here to be significantly bluer than previous studies have indicated. The luminosity function of the cluster is studied down to V = 22.8 (Mroughly-equal7.5) and shown to be consistent with a power-law mass function, n(M) = QM/sup -alpha/ with α = 2.5 +- 1.0, to the limit of our data. The field star population brighter than V = 21.5, is examined in some detail. There appears to be about 50% more stars belonging to the disk in the field as compared with the Bahcall-Soneira standard galaxy model. The reddening to the cluster is found to be E(B-V) = 0.11 +- 0.04 from nine bright field stars. A new value for the ultraviolet excess of the cluster main-sequence stars is obtained, delta(0.6) = 0.25 +- 0.02, and confirms the well-known fact that M15 is among the metal poorest of the globular clusters

Development of CCD Imaging System Using Thermoelectric Cooling Method

Directory of Open Access Journals (Sweden)

Youngsik Park

2000-06-01

Full Text Available We developed low light CCD imaging system using thermoelectric cooling method collaboration with a company to design a commercial model. It consists of Kodak KAF-0401E (768x512 pixels CCD chip,thermoelectric module manufactured by Thermotek. This TEC system can reach an operative temperature of -25deg. We employed an Uniblitz VS25S shutter and it has capability a minimum exposure time 80ms. The system components are an interface card using a Korea Astronomy Observatory (hereafter KAO ISA bus controller, image acquisition with AD9816 chip, that is 12bit video processor. The performance test with this imaging system showed good operation within the initial specification of our design. It shows a dark current less than 0.4e-/pixel/sec at a temperature of -10deg, a linearity 99.9+/-0.1%, gain 4.24e-adu, and system noise is 25.3e- (rms. For low temperature CCD operation, we designed a TEC, which uses a one-stage peltier module and forced air heat exchanger. This TEC imaging system enables accurate photometry (+/-0.01mag even though the CCD is not at 'conventional' cryogenic temperatures (140K. The system can be a useful instrument for any other imaging applications. Finally, with this system, we obtained several images of astronomical objects for system performance tests.

THE AGE-METALLICITY RELATIONSHIP OF THE LARGE MAGELLANIC CLOUD FIELD STAR POPULATION FROM WIDE-FIELD WASHINGTON PHOTOMETRY

International Nuclear Information System (INIS)

Piatti, Andrés E.; Geisler, Doug

2013-01-01

We analyze age and metallicity estimates for an unprecedented database of some 5.5 million stars distributed throughout the Large Magellanic Cloud (LMC) main body, obtained from CCD Washington CT 1 photometry, reported on in Piatti et al. We produce a comprehensive field star age-metallicity relationship (AMR) from the earliest epoch until ∼1 Gyr ago. This AMR reveals that the LMC has not evolved chemically as either a closed-box or bursting system, exclusively, but as a combination of both scenarios that have varied in relative strength over the lifetime of the galaxy, although the bursting model falls closer to the data in general. Furthermore, while old and metal-poor field stars have been preferentially formed in the outer disk, younger and more metal-rich stars have mostly been formed in the inner disk, confirming an outside-in formation. We provide evidence for the formation of stars between 5 and 12 Gyr, during the cluster age gap, although chemical enrichment during this period was minimal. We find no significant metallicity gradient in the LMC. We also find that the range in the metallicity of an LMC field has varied during the lifetime of the LMC. In particular, we find only a small range of the metal abundance in the outer disk fields, whereas an average range of Δ[Fe/H] = +0.3 ± 0.1 dex appears in the inner disk fields. Finally, the cluster and field AMRs show a satisfactory match only for the last 3 Gyr, while for the oldest ages (>11 Gyr), the cluster AMR is a remarkable lower envelope to the field AMR. Such a difference may be due to the very rapid early chemical evolution and lack of observed field stars in this regime, whereas the globular clusters are easily studied. This large difference is not easy to explain as coming from stripped ancient Small Magellanic Cloud (SMC) clusters, although the field SMC AMR is on average ∼0.4 dex more metal-poor at all ages than that of the LMC but otherwise very similar.

THE AGE-METALLICITY RELATIONSHIP OF THE LARGE MAGELLANIC CLOUD FIELD STAR POPULATION FROM WIDE-FIELD WASHINGTON PHOTOMETRY

Energy Technology Data Exchange (ETDEWEB)

Piatti, Andres E. [Instituto de Astronomia y Fisica del Espacio, CC 67, Suc. 28, 1428 Ciudad de Buenos Aires (Argentina); Geisler, Doug, E-mail: andres@iafe.uba.ar [Departamento de Astronomia, Universidad de Concepcion, Casilla 160-C, Concepcion (Chile)

2013-01-01

We analyze age and metallicity estimates for an unprecedented database of some 5.5 million stars distributed throughout the Large Magellanic Cloud (LMC) main body, obtained from CCD Washington CT{sub 1} photometry, reported on in Piatti et al. We produce a comprehensive field star age-metallicity relationship (AMR) from the earliest epoch until {approx}1 Gyr ago. This AMR reveals that the LMC has not evolved chemically as either a closed-box or bursting system, exclusively, but as a combination of both scenarios that have varied in relative strength over the lifetime of the galaxy, although the bursting model falls closer to the data in general. Furthermore, while old and metal-poor field stars have been preferentially formed in the outer disk, younger and more metal-rich stars have mostly been formed in the inner disk, confirming an outside-in formation. We provide evidence for the formation of stars between 5 and 12 Gyr, during the cluster age gap, although chemical enrichment during this period was minimal. We find no significant metallicity gradient in the LMC. We also find that the range in the metallicity of an LMC field has varied during the lifetime of the LMC. In particular, we find only a small range of the metal abundance in the outer disk fields, whereas an average range of {Delta}[Fe/H] = +0.3 {+-} 0.1 dex appears in the inner disk fields. Finally, the cluster and field AMRs show a satisfactory match only for the last 3 Gyr, while for the oldest ages (>11 Gyr), the cluster AMR is a remarkable lower envelope to the field AMR. Such a difference may be due to the very rapid early chemical evolution and lack of observed field stars in this regime, whereas the globular clusters are easily studied. This large difference is not easy to explain as coming from stripped ancient Small Magellanic Cloud (SMC) clusters, although the field SMC AMR is on average {approx}0.4 dex more metal-poor at all ages than that of the LMC but otherwise very similar.

Research of optical coherence tomography microscope based on CCD detector

Science.gov (United States)

Zhang, Hua; Xu, Zhongbao; Zhang, Shuomo

2008-12-01

The reference wave phase was modulated with a sinusoidal vibrating mirror attached to a Piezoelectric Transducer (PZT), the integration was performed by a CCD, and the charge storage period of the CCD image sensor was one-quarter period of the sinusoidal phase modulation. With the frequency- synchronous detection technique, four images (four frames of interference pattern) were recorded during one period of the phase modulation. In order to obtain the optimum modulation parameter, the values of amplitude and phase of the sinusoidal phase modulation were determined by considering the measurement error caused by the additive noise contained in the detected values. The PZT oscillation was controlled by a closed loop control system based on PID controller. An ideal discrete digital sine function at 50Hz with adjustable amplitude was used to adjust the vibrating of PZT, and a digital phase shift techniques was used to adjust vibrating phase of PZT so that the phase of the modulation could reach their optimum values. The CCD detector was triggered with software at 200Hz. Based on work above a small coherent signal masked by the preponderant incoherent background with a CCD detector was obtained.

Wide-field absolute transverse blood flow velocity mapping in vessel centerline

Science.gov (United States)

Wu, Nanshou; Wang, Lei; Zhu, Bifeng; Guan, Caizhong; Wang, Mingyi; Han, Dingan; Tan, Haishu; Zeng, Yaguang

2018-02-01

We propose a wide-field absolute transverse blood flow velocity measurement method in vessel centerline based on absorption intensity fluctuation modulation effect. The difference between the light absorption capacities of red blood cells and background tissue under low-coherence illumination is utilized to realize the instantaneous and average wide-field optical angiography images. The absolute fuzzy connection algorithm is used for vessel centerline extraction from the average wide-field optical angiography. The absolute transverse velocity in the vessel centerline is then measured by a cross-correlation analysis according to instantaneous modulation depth signal. The proposed method promises to contribute to the treatment of diseases, such as those related to anemia or thrombosis.

Cucurbits depicted in Byzantine mosaics from Israel, 350–600 ce

Science.gov (United States)

Avital, Anat; Paris, Harry S.

2014-01-01

Background and Aims Thousands of floor mosaics were produced in lands across the Roman and Byzantine empires. Some mosaics contain depictions of agricultural produce, potentially providing useful information concerning the contemporary presence and popularity of crop plants in a particular geographical region. Hundreds of floor mosaics produced in Israel during the Byzantine period have survived. The objective of the present work was to search these mosaics for Cucurbitaceae in order to obtain a more complete picture of cucurbit crop history in the eastern Mediterranean region. Results and Conclusions Twenty-three mosaics dating from 350–600 ce were found that had images positively identifiable as cucurbits. The morphological diversity of the cucurbit fruits in the mosaics of Israel is greater than that appearing in mosaics from any other Roman or Byzantine provincial area. The depicted fruits vary in shape from oblate to extremely long, and some are furrowed, others are striped and others lack definite markings. The cucurbit taxa depicted in the mosaics are Cucumis melo (melon), Citrullus lanatus (watermelon), Luffa aegyptiaca (sponge gourd) and Lagenaria siceraria (bottle gourd). Cucumis melo is the most frequently found taxon in the mosaics and is represented by round dessert melons and long snake melons. Fruits of at least two cultivars of snake melons and of watermelons are represented. To our knowledge, images of sponge gourds have not been found in Roman and Byzantine mosaics elsewhere. Indeed, the mosaics of Israel contain what are probably the oldest depictions of Luffa aegyptiaca in Mediterranean lands. Sponge gourds are depicted often, in 11 of the mosaics at eight localities, and the images include both mature fruits, which are useful for cleaning and washing, and immature fruits, which are edible. Only one mosaic has images positively identifiable as of bottle gourds, and these were round–pyriform and probably used as vessels. PMID:24948671

Miniature CCD X-Ray Imaging Camera Technology Final Report CRADA No. TC-773-94

Energy Technology Data Exchange (ETDEWEB)

Conder, A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Mummolo, F. J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2017-10-19

The goal of the project was to develop a compact, large active area, high spatial resolution, high dynamic range, charge-coupled device (CCD) camera to replace film for digital imaging of visible light, ultraviolet radiation, and soft to penetrating X-rays. The camera head and controller needed to be capable of operation within a vacuum environment and small enough to be fielded within the small vacuum target chambers at LLNL.

In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy.

Directory of Open Access Journals (Sweden)

Yuichiro Hayashi

Full Text Available A combination of genetically-encoded calcium indicators and micro-optics has enabled monitoring of large-scale dynamics of neuronal activity from behaving animals. In these studies, wide-field microscopy is often used to visualize neural activity. However, this method lacks optical sectioning capability, and therefore its axial resolution is generally poor. At present, it is unclear whether wide-field microscopy can visualize activity of densely packed small neurons at cellular resolution. To examine the applicability of wide-field microscopy for small-sized neurons, we recorded calcium activity of dentate granule cells having a small soma diameter of approximately 10 micrometers. Using a combination of high numerical aperture (0.8 objective lens and independent component analysis-based image segmentation technique, activity of putative single granule cell activity was separated from wide-field calcium imaging data. The result encourages wider application of wide-field microscopy in in vivo neurophysiology.

Occurrence of Cucumber mosaic virus on vanilla

Indian Academy of Sciences (India)

Cucumber mosaic virus (CMV) causing mosaic, leaf distortion and stunting of vanilla (Vanilla planifolia Andrews) in India was characterized on the basis of biological and coat protein (CP) nucleotide sequence properties. In mechanical inoculation tests, the virus was found to infect members of Chenopodiaceae, ...

Investigation of radiation damage effects in neutron irradiated CCD

International Nuclear Information System (INIS)

Brau, James E.; Igonkina, Olga; Potter, Chris T.; Sinev, Nikolai B.

2005-01-01

A Charge Coupled Devices (CCD)-based vertex detector is a leading option for vertex detection at the future linear collider. A major issue for this application is the radiation hardness of such devices. Tests of radiation hardness of CCDs used in the SLD vertex detector, VXD3, have been reported earlier. The first measurements of 1998 involved a spare VXD3 CCD that was irradiated with neutrons from a radioactive source (Pu-Be), and from a nuclear reactor. In 2003, we had the opportunity to disassemble the VXD3 detector and study the nature of the radiation damage it incurred during 3 years of operation at SLC. In the preparation for this study, additional experiments with the spare VXD3 CCD were performed. These included measurements of trapping times in neutron irradiated CCDs. Results, reported here, will help us better understand the mechanism of radiation damage effects and develop techniques to minimize performance degradation due to radiation damage

A CCD camera probe for a superconducting cyclotron

International Nuclear Information System (INIS)

Marti, F.; Blue, R.; Kuchar, J.; Nolen, J.A.; Sherrill, B.; Yurkon, J.

1991-01-01

The traditional internal beam probes in cyclotrons have consisted of a differential element, a wire or thin strip, and a main probe with several fingers to determine the vertical distribution of the beam. The resolution of these probes is limited, especially in the vertical direction. The authors have developed a probe for their K1200 superconducting cyclotron based on a CCD TV camera that works in a 6 T magnetic field. The camera looks at the beam spot on a scintillating screen. The TV image is processed by a frame grabber that digitizes and displays the image in pseudocolor in real time. This probe has much better resolution than traditional probes. They can see beams with total currents as low as 0.1 pA, with position resolution of about 0.05 mm

Mosaic Messages

Science.gov (United States)

Baldauf, Annemarie

2012-01-01

Through the generosity of a Lowes Toolbox for Education Grant and a grant from the Bill Graham Foundation, an interdisciplinary mosaic mural was created and installed at Riverview Middle School in Bay Point, California. The actual mural, which featured a theme of nurturing students through music, art, sports, science, and math, took about three…

Constitutional trisomy 8 mosaicism syndrome: case report and review.

Science.gov (United States)

Udayakumar, Achandira M; Al-Kindy, Adila

2013-12-01

Trisomy 8 mosaicism (Warkany syndrome) is a rare viable condition with variable phenotypes, ranging from mild dysmorphic features to severe malformations. Karyotyping and fluorescence in-situ hybridization potentially help detecting this low mosaic clone to confirm the diagnosis of patients with classical and unusual clinical presentations. This report reviews few previous cases to describe our case - a boy who had trisomy 8 mosaicism with severe dysmorphic features, born to a consanguineous Arabic couple. This study concludes that careful cytogenetic diagnoses of trisomy 8 mosaicism is essential for appropriate management and follow up of this rare disorder.

Stroboscope Based Synchronization of Full Frame CCD Sensors.

Science.gov (United States)

Shen, Liang; Feng, Xiaobing; Zhang, Yuan; Shi, Min; Zhu, Dengming; Wang, Zhaoqi

2017-04-07

The key obstacle to the use of consumer cameras in computer vision and computer graphics applications is the lack of synchronization hardware. We present a stroboscope based synchronization approach for the charge-coupled device (CCD) consumer cameras. The synchronization is realized by first aligning the frames from different video sequences based on the smear dots of the stroboscope, and then matching the sequences using a hidden Markov model. Compared with current synchronized capture equipment, the proposed approach greatly reduces the cost by using inexpensive CCD cameras and one stroboscope. The results show that our method could reach a high accuracy much better than the frame-level synchronization of traditional software methods.

The study of interferometer spectrometer based on DSP and linear CCD

Science.gov (United States)

Kang, Hua; Peng, Yuexiang; Xu, Xinchen; Xing, Xiaoqiao

2010-11-01

In this paper, general theory of Fourier-transform spectrometer and polarization interferometer is presented. A new design is proposed for Fourier-transform spectrometer based on polarization interferometer with Wollaston prisms and linear CCD. Firstly, measured light is changed into linear polarization light by polarization plate. And then the light can be split into ordinary and extraordinary lights by going through one Wollaston prism. At last, after going through another Wollaston prism and analyzer, interfering fringes can be formed on linear CCD behind the analyzer. The linear CCD is driven by CPLD to output amplitude of interfering fringes and synchronous signals of frames and pixels respectively. DSP is used to collect interference pattern signals from CCD and the digital data of interfering fringes are processed by using 2048-point-FFT. Finally, optical spectrum of measured light can be display on LCD connected to DSP with RS232. The spectrometer will possess the features of firmness, portability and the ability of real-time analyzing. The work will provide a convenient and significant foundation for application of more high accuracy of Fourier-transform spectrometer.

Análise de mapas de represas publicados na web usando imagens orbitais CCD/CBERS no estado de Minas Gerais Analysis of dams maps published on the web by using orbital CCD/CBERS images in Minas Gerais State, Brazil

Directory of Open Access Journals (Sweden)

Elizabeth Ferreira

2010-02-01

Full Text Available Neste trabalho, bancos de dados públicos e gratuitos disponíveis na World Wide Web (WEB foram utilizados para avaliar as áreas das superfícies dos espelhos d'água das represas de Furnas e do Funil, no Estado de Minas Gerais. O objetivo foi comparar as informações obtidas nos bancos da WEB com os valores das áreas calculadas a partir de imagens do sensor CCD a bordo dos satélites CBERS2 e CBERS2B. A área da represa de Furnas obtida a partir das imagens CCD/CBERS2B, ano 2008, foi de 1.138 km², mas nos bancos de dados consultados esta área estava entre 1.182 e 1.503 km². A represa do Funil, construída em 2003, com superfície de espelho d'água de 29,37 km² e uma ilha com área de 1,93 km² não aparecem nos bancos Atlas, Geominas, IGAM e IBGE. Os resultados mostraram algumas discrepâncias nos bancos de dados publicados na WEB, tais como diferenças em áreas e supressão ou extrapolação de limites do espelho d'água. Concluiu-se que, até o momento, os responsáveis por algumas publicações de bancos de dados no Estado de Minas Gerais não tiveram rigor suficiente com as atualizações. As imagens CCD/CBERS, que também são dados públicos disponíveis na WEB, mostraram ser produtos adequados para verificar, atualizar e melhorar as informações publicadas.In this work some public databases from the World Wide Web (WEB were used to find the area of the Furnas and Funil Dams in Minas Gerais State. The purpose of this work was to compare the WEB public databases values and the real values obtained from the CCD camera images on board CBERS2 and CBERS2B satellites. The Furnas Dam area obtained from CCD/ CBERS2B images, in 2008, was 1.138 km², but in the consulted databases this area ranged from 1.182 to 1.503 km². The dam of the Funil, built in 2003, with a water surface of 29.37 km² and an island with 1.93 km² area, did not appear in Atlas, Geominas, IGAM and IBGE databases. The results revealed some problems in the WEB public

Ultra-wide-field angiography improves the detection and classification of diabetic retinopathy.

Science.gov (United States)

Wessel, Matthew M; Aaker, Grant D; Parlitsis, George; Cho, Minhee; D'Amico, Donald J; Kiss, Szilárd

2012-04-01

To evaluate patients with diabetic retinopathy using ultra-wide-field fluorescein angiography and to compare the visualized retinal pathology with that seen on an overly of conventional 7 standard field (7SF) imaging. Two hundred and eighteen eyes of 118 diabetic patients who underwent diagnostic fluorescein angiography using the Optos Optomap Panoramic 200A imaging system were included. The visualized area of the retina, retinal nonperfusion, retinal neovascularization, and panretinal photocoagulation were quantified by two independent masked graders. The respective areas identified on the ultra-wide-field fluorescein angiography image were compared with an overly of a modified 7SF image as outlined in the Early Treatment Diabetic Retinopathy Study. Ultra-wide-field fluorescein angiograms imaging, on average, demonstrated 3.2 times more total retinal surface area than 7SF. When compared with 7SF, ultra-wide-field fluorescein angiography showed 3.9 times more nonperfusion (P diabetic retinopathy. Improved retinal visualization may alter the classification of diabetic retinopathy and may therefore influence follow-up and treatment of these patients.

Wide-field surveys from the SNAP mission

International Nuclear Information System (INIS)

2002-01-01

The Supernova/Acceleration Probe (SNAP) is a proposed space-borne observatory that will survey the sky with a wide-field optical/NIR imager. The images produced by SNAP will have an unprecedented combination of depth, solid-angle, angular resolution, and temporal sampling. Two 7.5 square-degree fields will be observed every four days over 16 months to a magnitude depth of AB = 27.7 in each of nine filters. Co-adding images over all epochs will give an AB = 30.3 per filter. A 300 square-degree field will be surveyed with no repeat visits to AB = 28 per filter. The nine filters span 3500-17000 (angstrom). Although the survey strategy is tailored for supernova and weak gravitational lensing observations, the resulting data supports a broad range of auxiliary science programs

CCD-based thermoreflectance microscopy: principles and applications

International Nuclear Information System (INIS)

Farzaneh, M; Maize, K; Shakouri, A; Lueerssen, D; Summers, J A; Hudgings, Janice A; Mayer, P M; Ram, R J; Raad, P E; Pipe, K P

2009-01-01

CCD-based thermoreflectance microscopy has emerged as a high resolution, non-contact imaging technique for thermal profiling and performance and reliability analysis of numerous electronic and optoelectronic devices at the micro-scale. This thermography technique, which is based on measuring the relative change in reflectivity of the device surface as a function of change in temperature, provides high-resolution thermal images that are useful for hot spot detection and failure analysis, mapping of temperature distribution, measurement of thermal transient, optical characterization of photonic devices and measurement of thermal conductivity in thin films. In this paper we review the basic physical principle behind thermoreflectance as a thermography tool, discuss the experimental setup, resolutions achieved, signal processing procedures and calibration techniques, and review the current applications of CCD-based thermoreflectance microscopy in various devices. (topical review)

Converting structures to optimize the Synchrotron X radiation detection by CCD systems

International Nuclear Information System (INIS)

Zanella, G.; Zannoni, R.

1987-01-01

It is pointed out how the quantum efficiency of X ray detection for CCD detecting system can be improved enlarging their sensivity range by means of heavy element converting structures. So the problem of fabricating CCD with a deep emptying layer is avoided

CCD photometry in the Vilnius photometric system. I. region in Lyra

International Nuclear Information System (INIS)

Boyle, R.P.; Smriglio, F.; Straizys, V.

1990-01-01

Three-dimensional photometric classification can be made for stars measured in the Vilnius seven-color intermediate-band system, even if they are reddened by dust. Such classification is important for questions relating to the structure and evolution of our Galaxy. Presented here for general use is CCD photometry of 231 field stars in Lyra observed with the 0.9 meter telescope of Kitt Peak National Observatory. The measurements are of higher accuracy than those of a photographic study in the same direction in Lyra for which they can also be used for a deeper calibration of the photographic photometry. The data sets cover two small fields each about 31 arcmin 2 and are complete to 90% for all stars up to the faint limit of V = 17.5

Crystal mosaic spread determination by slow neutron scattering

International Nuclear Information System (INIS)

Adib, M.; Naguib, K.; Abdel Kawy, A.; Ashry, A.; Abbas, Y.; Wahba, M.; Maayouf, M.A.

1988-01-01

A method has been established for determination of the crystal mosaic spread. The method is based on recording all neutron-reflected, under bragg condition, from a certain crystal plane. A computer code was developed especially in order to fit the measured wavelength's distribution of the reflected neutrons with the calculated one, assuming that the crystal mosaic spread has a Gaussian shape. The code accounts for the parameters of the time of flight spectrometer used during the present measurements, as well as divergence of the incident neutron beam. The developed method has been applied for determination of the mosaic spread of both zinc and pyrolytic graphite (P.G.) crystals. The mosaic spread values deduced from the present measurements, are 10'+-6' and 3.60 0 +-0.16 0 respectively for Zn and P.G. crystals

A Design and Development of Multi-Purpose CCD Camera System with Thermoelectric Cooling: Software

Directory of Open Access Journals (Sweden)

S. H. Oh

2007-12-01

Full Text Available We present a software which we developed for the multi-purpose CCD camera. This software can be used on the all 3 types of CCD - KAF-0401E (768×512, KAF-1602E (15367times;1024, KAF-3200E (2184×1472 made in KODAK Co.. For the efficient CCD camera control, the software is operated with two independent processes of the CCD control program and the temperature/shutter operation program. This software is designed to fully automatic operation as well as manually operation under LINUX system, and is controled by LINUX user signal procedure. We plan to use this software for all sky survey system and also night sky monitoring and sky observation. As our results, the read-out time of each CCD are about 15sec, 64sec, 134sec for KAF-0401E, KAF-1602E, KAF-3200E., because these time are limited by the data transmission speed of parallel port. For larger format CCD, the data transmission is required more high speed. we are considering this control software to one using USB port for high speed data transmission.

40 CFR 174.514 - Coat Protein of Watermelon Mosaic Virus-2 and Zucchini Yellow Mosaic Virus; exemption from the...

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Coat Protein of Watermelon Mosaic Virus-2 and Zucchini Yellow Mosaic Virus; exemption from the requirement for a tolerance. 174.514 Section 174.514 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR PLANT-INCORPORATED...

SPECIFICITY OF THE PRECIPITIN REACTION IN TOBACCO MOSAIC DISEASE.

Science.gov (United States)

Beale, H P

1931-09-30

1. Leaf extracts of Sudan grass, Hippeastrum equestre Herb., lily, and Abutilon striatum Dicks. (A. Thompsoni hort.), each affected with its respective mosaic disease, and peach affected with yellows disease, were tested for their ability to precipitate antiserum for virus extract of tobacco mosaic disease. No precipitate occurred. 2. Nicotiana glutinosa L., N. rustica L., and Martynia louisiana Mill. were added to the list of hosts of tobacco mosaic virus which have been tested with antiserum for the same virus in N. tabacum L. var. Turkish. The object was to determine the presence or absence of material reacting with the specific precipitins such as that already demonstrated in extracts of tomato, pepper, and petunia affected with the same virus. The presence of specific substances was demonstrated in every case. 3. The viruses of ringspot and cucumber mosaic diseases were multiplied in Turkish tobacco and leaf extracts of the affected plants were used in turn as antigens in precipitin tests with antiserum for tobacco mosaic virus extract of Turkish tobacco. A slight precipitation resulted in the tubes containing undiluted antiserum and virus extract such as occurs when juice from normal tobacco is used with undiluted antiserum. No precipitate was demonstrable that was specific for virus extracts of tobacco affected with either ringspot or cucumber mosaic disease. 4. The results favor the interpretation that the specific antigenic substance in virus extract of tobacco mosaic disease is foreign antigenic material, possibly virus itself, not altered host protein.

Deepest Wide-Field Colour Image in the Southern Sky

Science.gov (United States)

2003-01-01

extensive optical observations were obtained in the course of a dozen observing periods during several years, it is also possible to perform studies of certain variable phenomena: * How many variable sources are seen and what are their types and properties? * How many supernovae are detected per time interval, i.e. what is the supernovae frequency at different cosmic epochs? * How do those processes depend on each other? This is just a short and very incomplete list of questions astronomers world-wide will address using all the complementary observations. No doubt that the coming studies of the Chandra Deep Field South - with this and other data - will be most exciting and instructive! Other wide-field images Other wide-field images from the WFI have been published in various ESO press releases during the past four years - they are also available at the WFI Photo Gallery . A collection of full-resolution files (TIFF-format) is available on a WFI CD-ROM . Technical Information The very extensive data reduction and colour image processing needed to produce these images were performed by Mischa Schirmer and Thomas Erben at the "Wide Field Expertise Center" of the Institut für Astrophysik und Extraterrestrische Forschung der Universität Bonn (IAEF) in Germany. It was done by means of a software pipeline specialised for reduction of multiple CCD wide-field imaging camera data. This pipeline is mainly based on publicly available software modules and algorithms ( EIS , FLIPS , LDAC , Terapix , Wifix ). The image was constructed from about 150 exposures in each of the following wavebands: B-band (centred at wavelength 456 nm; here rendered as blue, 15.8 hours total exposure time), V-band (540 nm; green, 15.6 hours) and R-band (652 nm; red, 17.8 hours). Only images taken under sufficiently good observing conditions (defined as seeing less than 1.1 arcsec) were included. In total, 450 images were assembled to produce this colour image, together with about as many calibration images

Design of offline measuring system for radiation damage effects on linear CCD

International Nuclear Information System (INIS)

Zhang Yong; Tang Benqi; Xiao Zhigang; Wang Zujun; Huang Fang; Huang Shaoyan

2004-01-01

The paper discusses the hardware design of offline measuring system for radiation damage effects on linear CCD. Some credible results were achieved by using this system. The test results indicate that the system is available for the study of the radiation damage effects on linear CCD. (authors)

On-ground and in-orbit characterisation plan for the PLATO CCD normal cameras

Science.gov (United States)

Gow, J. P. D.; Walton, D.; Smith, A.; Hailey, M.; Curry, P.; Kennedy, T.

2017-11-01

PLAnetary Transits and Ocillations (PLATO) is the third European Space Agency (ESA) medium class mission in ESA's cosmic vision programme due for launch in 2026. PLATO will carry out high precision un-interrupted photometric monitoring in the visible band of large samples of bright solar-type stars. The primary mission goal is to detect and characterise terrestrial exoplanets and their systems with emphasis on planets orbiting in the habitable zone, this will be achieved using light curves to detect planetary transits. PLATO uses a novel multi- instrument concept consisting of 26 small wide field cameras The 26 cameras are made up of a telescope optical unit, four Teledyne e2v CCD270s mounted on a focal plane array and connected to a set of Front End Electronics (FEE) which provide CCD control and readout. There are 2 fast cameras with high read-out cadence (2.5 s) for magnitude ~ 4-8 stars, being developed by the German Aerospace Centre and 24 normal (N) cameras with a cadence of 25 s to monitor stars with a magnitude greater than 8. The N-FEEs are being developed at University College London's Mullard Space Science Laboratory (MSSL) and will be characterised along with the associated CCDs. The CCDs and N-FEEs will undergo rigorous on-ground characterisation and the performance of the CCDs will continue to be monitored in-orbit. This paper discusses the initial development of the experimental arrangement, test procedures and current status of the N-FEE. The parameters explored will include gain, quantum efficiency, pixel response non-uniformity, dark current and Charge Transfer Inefficiency (CTI). The current in-orbit characterisation plan is also discussed which will enable the performance of the CCDs and their associated N-FEE to be monitored during the mission, this will include measurements of CTI giving an indication of the impact of radiation damage in the CCDs.

Optimization of polarimetry sensitivity for X-ray CCD

CERN Document Server

Hayashida, K; Tsunemi, H; Hashimoto, Y; Ohtani, M

1999-01-01

X-ray polarimetry with CCD has been performed using a polarized X-ray beam from an electron impact X-ray source. The standard data reduction method employing double-pixel events yields the modulation factor M of 0.14 at 27 keV and 0.24 at 43 keV for the 12 mu m pixel size CCD chip. We develop a new data reduction method, in which multi-pixel events are employed, and which approximates the charge spread as an oval shape. We optimize the reduction parameters, so that we improve the P sub m sub i sub n (minimum detectable polarization degree) by factor of three from the value obtained through the usual double-pixel event method.

Stroboscope Based Synchronization of Full Frame CCD Sensors

Directory of Open Access Journals (Sweden)

Liang Shen

2017-04-01

Full Text Available The key obstacle to the use of consumer cameras in computer vision and computer graphics applications is the lack of synchronization hardware. We present a stroboscope based synchronization approach for the charge-coupled device (CCD consumer cameras. The synchronization is realized by first aligning the frames from different video sequences based on the smear dots of the stroboscope, and then matching the sequences using a hidden Markov model. Compared with current synchronized capture equipment, the proposed approach greatly reduces the cost by using inexpensive CCD cameras and one stroboscope. The results show that our method could reach a high accuracy much better than the frame-level synchronization of traditional software methods.

Modelling charge storage in Euclid CCD structures

International Nuclear Information System (INIS)

Clarke, A S; Holland, A; Hall, D J; Burt, D

2012-01-01

The primary aim of ESA's proposed Euclid mission is to observe the distribution of galaxies and galaxy clusters, enabling the mapping of the dark architecture of the universe [1]. This requires a high performance detector, designed to endure a harsh radiation environment. The e2v CCD204 image sensor was redesigned for use on the Euclid mission [2]. The resulting e2v CCD273 has a narrower serial register electrode and transfer channel compared to its predecessor, causing a reduction in the size of charge packets stored, thus reducing the number of traps encountered by the signal electrons during charge transfer and improving the serial Charge Transfer Efficiency (CTE) under irradiation [3]. The proposed Euclid CCD has been modelled using the Silvaco TCAD software [4], to test preliminary calculations for the Full Well Capacity (FWC) and the channel potential of the device and provide indications of the volume occupied by varying signals. These results are essential for the realisation of the mission objectives and for radiation damage studies, with the aim of producing empirically derived formulae to approximate signal-volume characteristics in the devices. These formulae will be used in the radiation damage (charge trapping) models. The Silvaco simulations have been tested against real devices to compare the experimental measurements to those predicted in the models. Using these results, the implications of this study on the Euclid mission can be investigated in more detail.

Molecular characterization and experimental host range of an isolate of Wissadula golden mosaic St. Thomas virus.

Science.gov (United States)

Collins, A M; Mujaddad-ur-Rehman, Malik; Brown, J K; Reddy, C; Wang, A; Fondong, V; Roye, M E

2009-12-01

Partial genome segments of a begomovirus were previously amplified from Wissadula amplissima exhibiting yellow-mosaic and leaf-curl symptoms in the parish of St. Thomas, Jamaica and this isolate assigned to a tentative begomovirus species, Wissadula golden mosaic St. Thomas virus. To clone the complete genome of this isolate of Wissadula golden mosaic St. Thomas virus, abutting primers were designed to PCR amplify its full-length DNA-A and DNA-B components. Sequence analysis of the complete begomovirus genome obtained, confirmed that it belongs to a distinct begomovirus species and this isolate was named Wissadula golden mosaic St. Thomas virus-[Jamaica:Albion:2005] (WGMSTV-[JM:Alb:05]). The genome of WGMSTV-[JM:Alb:05] is organized similar to that of other bipartite Western Hemisphere begomoviruses. Phylogenetic analyses placed the genome components of WGMSTV-[JM:Alb:05] in the Abutilon mosaic virus clade and showed that the DNA-A component is most closely related to four begomovirus species from Cuba, Tobacco leaf curl Cuba virus, Tobacco leaf rugose virus, Tobacco mottle leaf curl virus, and Tomato yellow distortion leaf virus. The putative Rep-binding-site motif in the common region of WGMSTV-[JM:Alb:05] was observed to be identical to that of Chino del tomate virus-Tomato [Mexico:Sinaloa:1983], Sida yellow mosaic Yucatan virus-[Mexico:Yucatan:2005], and Tomato leaf curl Sinaloa virus-[Nicaragua:Santa Lucia], suggesting that WGMSTV-[JM:Alb:05] is capable of forming viable pseudo-recombinants with these begomoviruses, but not with other members of the Abutilon mosaic virus clade. Biolistic inoculation of test plant species with partial dimers of the WGMSTV-[JM:Alb:05] DNA-A and DNA-B components showed that the virus was infectious to Nicotiana benthamiana and W. amplissima and the cultivated species Phaseolus vulgaris (kidney bean) and Lycopersicon esculentum (tomato). Infected W. amplissima plants developed symptoms similar to symptoms observed under field

Stellar CCD Photometry: New Approach, Principles and Application

Science.gov (United States)

El-Bassuny Alawy, A.

A new approach is proposed and developed to handle pre-processed CCD frames in order to identify stellar images and derive their relevant parameters. It relies on: 1) Identifying stellar images and assigning approximate positions of their centres using an artificial intelligence technique, (Knowledge Based System), 2) Accurate determination of the centre co-ordinates applying an elementary statistical concept and 3) Estimating the image peak intensity as a stellar magnitude measure employing simple numerical analysis approach. The method has been coded for personal computer users. A CCD frame of the star cluster M67 was adopted as a test case. The results obtained are discussed in comparison with the DAOPHOTII ones and the corresponding published data. Exact coincidence has been found between both results except in very few cases. These exceptions have been discussed in the light of the basis of both methods and the cluster plates. It has been realised that the method suggested represents a very simple, extremely fast, high precision method of stellar CCD photometry. Moreover, it is more capable than DAOPHOTII of handling blended and distorted stellar images. These characteristics show the usefulness of the present method in some astronomical applications, such as auto-focusing and auto-guiding, beside the main purpose, viz. stellar photometry.

Optical CT scanning of PRESAGETM polyurethane samples with a CCD-based readout system

International Nuclear Information System (INIS)

Doran, S J; Krstajic, N; Adamovics, J; Jenneson, P M

2004-01-01

This article demonstrates the resolution capabilities of the CCD scanner under ideal circumstances and describes the first CCD-based optical CT experiments on a new class of dosimeter, known as PRESAGE TM (Heuris Pharma, Skillman, NJ)

Virus-induced gene silencing in diverse maize lines using the Brome Mosaic virus-based silencing vector

Science.gov (United States)

Virus-induced gene silencing (VIGS) is a widely used tool for gene function studies in many plant species, though its use in monocots has been limited. Using a Brome mosaic virus (BMV) vector designed to silence the maize phytoene desaturase gene, a genetically diverse set of maize inbred lines was ...

Non-mydriatic, wide field, fundus video camera

Science.gov (United States)

Hoeher, Bernhard; Voigtmann, Peter; Michelson, Georg; Schmauss, Bernhard

2014-02-01

We describe a method we call "stripe field imaging" that is capable of capturing wide field color fundus videos and images of the human eye at pupil sizes of 2mm. This means that it can be used with a non-dilated pupil even with bright ambient light. We realized a mobile demonstrator to prove the method and we could acquire color fundus videos of subjects successfully. We designed the demonstrator as a low-cost device consisting of mass market components to show that there is no major additional technical outlay to realize the improvements we propose. The technical core idea of our method is breaking the rotational symmetry in the optical design that is given in many conventional fundus cameras. By this measure we could extend the possible field of view (FOV) at a pupil size of 2mm from a circular field with 20° in diameter to a square field with 68° by 18° in size. We acquired a fundus video while the subject was slightly touching and releasing the lid. The resulting video showed changes at vessels in the region of the papilla and a change of the paleness of the papilla.

Novel X-ray telescopes for wide-field X-ray monitoring

International Nuclear Information System (INIS)

Hudec, R.; Inneman, A.; Pina, L.; Sveda, L.

2005-01-01

We report on fully innovative very wide-field of view X-ray telescopes with high sensitivity as well as large field of view. The prototypes are very promising, allowing the proposals for space projects with very wide-field Lobster-eye X-ray optics to be considered. The novel telescopes will monitor the sky with unprecedented sensitivity and angular resolution of order of 1 arcmin. They are expected to contribute essentially to study and to understand various astrophysical objects such as AGN, SNe, Gamma-ray bursts (GRBs), X-ray flashes (XRFs), galactic binary sources, stars, CVs, X-ray novae, various transient sources, etc. The Lobster optics based X-ray All Sky Monitor is capable to detect around 20 GRBs and 8 XRFs yearly and this will surely significantly contribute to the related science

Depth-resolved incoherent and coherent wide-field high-content imaging (Conference Presentation)

Science.gov (United States)

So, Peter T.

2016-03-01

Recent advances in depth-resolved wide-field imaging technique has enabled many high throughput applications in biology and medicine. Depth resolved imaging of incoherent signals can be readily accomplished with structured light illumination or nonlinear temporal focusing. The integration of these high throughput systems with novel spectroscopic resolving elements further enable high-content information extraction. We will introduce a novel near common-path interferometer and demonstrate its uses in toxicology and cancer biology applications. The extension of incoherent depth-resolved wide-field imaging to coherent modality is non-trivial. Here, we will cover recent advances in wide-field 3D resolved mapping of refractive index, absorbance, and vibronic components in biological specimens.

A fast CCD detector for charge exchange recombination spectroscopy on the DIII-D tokamak

International Nuclear Information System (INIS)

Thomas, D.M.; Burrell, K.H.; Groebner, R.J.; Gohil, P.

1996-05-01

Charge Exchange Recombination (CER) spectroscopy has become a standard diagnostic for tokamaks. CER measurements have been used to determine spatially and temporally resolved ion temperature, toroidal and poloidal ion rotation speed, impurity density and radial electric field. Knowledge of the spatial profile and temporal evolution of the electric field shear in the plasma edge is crucial to understanding the physics of the L to H transition. High speed CER measurements are also valuable for Edge Localized Mode (ELM) studies. Since the 0.52 ms minimum time resolution of our present system is barely adequate to study the time evolution of these phenomena, we have developed a new CCD detector system with about a factor of two better time resolution. In addition, our existing system detects sufficient photons to utilize the shortest time resolution only under exceptional conditions. The new CCD detector has a quantum efficiency of about 0.65, which is a factor of 7 better than our previous image intensifier-silicon photodiode detector systems. We have also equipped the new system with spectrometers of lower f/number. This combination should allow more routine operation at the minimum integration time, as well as improving data quality for measurements in the divertor-relevant region outside of the separatrix. Construction details, benchmark data and initial tokamak measurements for the new system will be presented

A mosaic-based approach is needed to conserve biodiversity in disturbed freshwater ecosystems

Science.gov (United States)

Hitchman, Sean M.; Mather, Martha E.; Smith, Joseph M.; Fencl, Jane S.

2017-01-01

Conserving native biodiversity in the face of human‐ and climate‐related impacts is a challenging and globally important ecological problem that requires an understanding of spatially connected, organismal‐habitat relationships. Globally, a suite of disturbances (e.g., agriculture, urbanization, climate change) degrades habitats and threatens biodiversity. A mosaic approach (in which connected, interacting collections of juxtaposed habitat patches are examined) provides a scientific foundation for addressing many disturbance‐related, ecologically based conservation problems. For example, if specific habitat types disproportionately increase biodiversity, these keystones should be incorporated into research and management plans. Our sampling of fish biodiversity and aquatic habitat along ten 3‐km sites within the Upper Neosho River subdrainage, KS, from June‐August 2013 yielded three generalizable ecological insights. First, specific types of mesohabitat patches (i.e., pool, riffle, run, and glide) were physically distinct and created unique mosaics of mesohabitats that varied across sites. Second, species richness was higher in riffle mesohabitats when mesohabitat size reflected field availability. Furthermore, habitat mosaics that included more riffles had greater habitat diversity and more fish species. Thus, riffles (<5% of sampled area) acted as keystone habitats. Third, additional conceptual development, which we initiate here, can broaden the identification of keystone habitats across ecosystems and further operationalize this concept for research and conservation. Thus, adopting a mosaic approach can increase scientific understanding of organismal‐habitat relationships, maintain natural biodiversity, advance spatial ecology, and facilitate effective conservation of native biodiversity in human‐altered ecosystems.

Pepino mosaic virus

NARCIS (Netherlands)

Vlugt, van der R.A.A.

2009-01-01

Pepino mosaic virus (PepMV) is a relatively new plant virus that has become a signifi cant agronomical problem in a relatively short period of time. It is a member of the genus Potexvirus within the family Flexiviridae and is readily mechanically transmissible. It is capable of infecting tomato

Apple mosaic virus

Science.gov (United States)

Apple mosaic virus (ApMV), a member of the ilarvirus group, naturally infects Betula, Aesculus, Humulus, and several crop genera in the family Rosaceae (Malus, Prunus, Rosa and Rubus). ApMV was first reported in Rubus in several blackberry and raspberry cultivars in the United States and subsequentl...

Chromosomal Mosaicism in Human Feto-Placental Development: Implications for Prenatal Diagnosis

Directory of Open Access Journals (Sweden)

Francesca Romana Grati

2014-07-01

Full Text Available Chromosomal mosaicism is one of the primary interpretative issues in prenatal diagnosis. In this review, the mechanisms underlying feto-placental chromosomal mosaicism are presented. Based on the substantial retrospective diagnostic experience with chorionic villi samples (CVS of a prenatal diagnosis laboratory the following items are discussed: (i The frequency of the different types of mosaicism (confined placental, CPM, and true fetal mosaicisms, TFM; (ii The risk of fetal confirmation after the detection of a mosaic in CVS stratified by chromosome abnormality and placental tissue involvement; (iii The frequency of uniparental disomy for imprinted chromosomes associated with CPM; (iv The incidence of false-positive and false-negative results in CVS samples analyzed by only (semi-direct preparation or long term culture; and (v The implications of the presence of a feto-placental mosaicism for microarray analysis of CVS and non-invasive prenatal screening (NIPS.

Ultrahigh-speed, high-sensitivity color camera with 300,000-pixel single CCD

Science.gov (United States)

Kitamura, K.; Arai, T.; Yonai, J.; Hayashida, T.; Ohtake, H.; Kurita, T.; Tanioka, K.; Maruyama, H.; Namiki, J.; Yanagi, T.; Yoshida, T.; van Kuijk, H.; Bosiers, Jan T.; Etoh, T. G.

2007-01-01

We have developed an ultrahigh-speed, high-sensitivity portable color camera with a new 300,000-pixel single CCD. The 300,000-pixel CCD, which has four times the number of pixels of our initial model, was developed by seamlessly joining two 150,000-pixel CCDs. A green-red-green-blue (GRGB) Bayer filter is used to realize a color camera with the single-chip CCD. The camera is capable of ultrahigh-speed video recording at up to 1,000,000 frames/sec, and small enough to be handheld. We also developed a technology for dividing the CCD output signal to enable parallel, highspeed readout and recording in external memory; this makes possible long, continuous shots up to 1,000 frames/second. As a result of an experiment, video footage was imaged at an athletics meet. Because of high-speed shooting, even detailed movements of athletes' muscles were captured. This camera can capture clear slow-motion videos, so it enables previously impossible live footage to be imaged for various TV broadcasting programs.

Research on Wide-field Imaging Technologies for Low-frequency Radio Array

Science.gov (United States)

Lao, B. Q.; An, T.; Chen, X.; Wu, X. C.; Lu, Y.

2017-09-01

Wide-field imaging of low-frequency radio telescopes are subject to a number of difficult problems. One particularly pernicious problem is the non-coplanar baseline effect. It will lead to distortion of the final image when the phase of w direction called w-term is ignored. The image degradation effects are amplified for telescopes with the wide field of view. This paper summarizes and analyzes several w-term correction methods and their technical principles. Their advantages and disadvantages have been analyzed after comparing their computational cost and computational complexity. We conduct simulations with two of these methods, faceting and w-projection, based on the configuration of the first-phase Square Kilometre Array (SKA) low frequency array. The resulted images are also compared with the two-dimensional Fourier transform method. The results show that image quality and correctness derived from both faceting and w-projection are better than the two-dimensional Fourier transform method in wide-field imaging. The image quality and run time affected by the number of facets and w steps have been evaluated. The results indicate that the number of facets and w steps must be reasonable. Finally, we analyze the effect of data size on the run time of faceting and w-projection. The results show that faceting and w-projection need to be optimized before the massive amounts of data processing. The research of the present paper initiates the analysis of wide-field imaging techniques and their application in the existing and future low-frequency array, and fosters the application and promotion to much broader fields.

A tilted fiber-optic plate coupled CCD detector for high resolution neutron imaging

Energy Technology Data Exchange (ETDEWEB)

Kim, Jongyul; Cho, Gyuseong [Korea Advanced Institute of Science and Technology, Daejeon (Korea, Republic of); Kim, Jongyul; Hwy, Limchang; Kim, Taejoo; Lee, Kyehong [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Lee, Seungwook [Pusan National Univ., Pusan (Korea, Republic of)

2013-05-15

One of these efforts is that a tilted scintillator geometry and lens coupled CCD detector for neutron imaging system were used to improve spatial resolution in one dimension. The increased spatial resolution in one dimension was applied to fuel cell study. However, a lens coupled CCD detector has lower sensitivity than a fiber-optic plate coupled CCD detector due to light loss. In this research, a tilted detector using fiber-optic plate coupled CCD detector was developed to improve resolution and sensitivity. In addition, a tilted detector can prevent an image sensor from direct radiation damage. Neutron imaging has been used for fuel cell study, lithium ion battery study, and many scientific applications. High quality neutron imaging is demanded for more detailed studies of applications, and spatial resolution should be considered to get high quality neutron imaging. Therefore, there were many efforts to improve spatial resolution.

Deep CCD photometry in globular clusters. VII. M30

International Nuclear Information System (INIS)

Richer, H.B.; Fahlman, G.G.; Vandenberg, D.A.

1988-01-01

New UBV CCD photometry in a single field of the globular cluster M30 was obtained, and the data were used to obtain the color magnitude diagram (CMD) of the cluster, its luminosity function, and to derive fundamental cluster parameters. No blue stragglers were found, nor any evidence of a binary sequence in the data even though the field under study is only 21 core radii from the cluster center. The cluster reddening is observed to be 0.068 + or - 0.035, significantly higher than that adopted in most current papers on M30. An intercomparison of the CMDs of three very metal-poor clusters clearly shows that there is no evidence for any age difference between them. The age of M30 itself is found to be about 14 Gyr. The luminosity function of M30 is determined to be M(V) = 8. Comparison of this function with one found by Bolte (1987) at 65 core radii shows clear evidence of mass segregation in the low-mass stars. 44 references

Association of a cucumber mosaic virus strain with mosaic disease of banana, Musa paradisiaca--an evidence using immuno/nucleic acid probe.

Science.gov (United States)

Srivastava, A; Raj, S K; Haq, Q M; Srivastava, K M; Singh, B P; Sane, P V

1995-12-01

Virus causing severe chlorosis/mosaic disease of banana was identified as a strain of cucumber mosaic virus (CMV). Association of CMV with the disease was established by Western immunoblot using polyclonal antibodies to CMV-T and slot blot hybridization with nucleic acid probe of CMV-P genome.

Maternal XX/X chromosome mosaicism in donor oocyte in vitro fertilization (IVF

Directory of Open Access Journals (Sweden)

Paul R. Brezina

2012-06-01

Results: The rates of maternal X chromosome mosaicism noted in the cycles from women with miscarriages (3%, 4%, 4%, and 6% were not statistically different from cycles in TS-Mosaic women with normal deliveries (3% and 11%. These data suggest that the rate of maternal X chromosome mosaicism does not affect pregnancy loss rates in TS-Mosaic women undergoing donor oocyte IVF.

X-ray astronomy 2000: Wide field X-ray monitoring with lobster-eye telescopes

International Nuclear Information System (INIS)

Inneman, A.; Hudec, R.; Pina, L.; Gorenstein, P.

2001-01-01

The recently available first prototypes of innovative very wide field X-ray telescopes of Lobster-Eye type confirm the feasibility to develop such flight instruments in a near future. These devices are expected to allow very wide field (more than 1000 square degrees) monitoring of the sky in X-rays (up to 10 keV and perhaps even more) with faint limits. We will discuss the recent status of the development of very wide field X-ray telescopes as well as related scientific questions including expected major contributions such as monitoring and study of X-ray afterglows of Gamma Ray Bursts

Calibration of the CCD photonic measuring system for railway inspection

Science.gov (United States)

Popov, D. V.; Ryabichenko, R. B.; Krivosheina, E. A.

2005-08-01

Increasing of traffic speed is the most important task in Moscow Metro. Requirements for traffic safety grow up simultaneously with the speed increasing. Currently for track inspection in Moscow Metro is used track measurement car has built in 1954. The main drawbacks of this system are absence of automated data processing and low accuracy. Non-contact photonic measurement system (KSIR) is developed for solving this problem. New track inspection car will be built in several months. This car will use two different track inspection systems and car locating subsystem based on track circuit counting. The KSIR consists of four subsystems: rail wear, height and track gauge measurement (BFSM); rail slump measurement (FIP); contact rail measurement (FKR); speed, level and car locating (USI). Currently new subsystem for wheel flange wear (IRK) is developed. The KSIR carry out measurements in real-time mode. The BFSM subsystem contains 4 matrix CCD cameras and 4 infrared stripe illuminators. The FIP subsystem contains 4 line CCD cameras and 4 spot illuminators. The FKR subsystem contains 2 matrix CCD cameras and 2 stripe illuminators. The IRK subsystem contains 2 CCD cameras and 2 stripe illuminators. Each system calibration was carried out for their adjustment. On the first step KSIR obtains data from photonic sensors which is valued in internal measurement units. Due to the calibration on the second step non-contact system converts the data to metric measurement system.

Identification of a monopartite begomovirus associated with yellow vein mosaic of Mentha longifolia in Saudi Arabia.

Science.gov (United States)

Sohrab, Sayed Sartaj; Daur, Ihsanullah

2018-02-01

Mentha is a very important crop grown and used extensively for many purposes in the Kingdom of Saudi Arabia. Begomoviruses are whitefly-transmitted viruses causing serious disease in many important plants exhibiting variable symptoms with significant economic loss globally. During farmers' field survey, yellow vein mosaic disease was observed in Mentha longifolia plants growing near tomato fields in Saudi Arabia. The causative agent was identified in 11 out of 19 samples using begomovirus-specific primers and the association of begomovirus with yellow vein mosaic disease in M. longifolia was confirmed. The full-length viral genome and betasatellite were amplified, cloned, and sequenced bidirectionally. The full DNA-A genome was found to have 2785 nucleotides with 1365 bp-associated betasatellite molecule. An attempt was made to amplify DNA-B, but none of the samples produced any positive amplicon of expected size which indicated the presence of monopartite begomovirus. The sequence identity matrix and phylogenetic analysis, based on full genome showed the highest identity (99.6%) with Tomato yellow leaf curl virus (TYLCV) and in phylogenetic analysis it formed a closed cluster with Tomato leaf curl virus infecting tomato and Corchorus crop in Saudi Arabia. The sequence analysis results of betasatellites showed the highest identity (98.9%) with Tomato yellow leaf curl betasatellites infecting tomato and phylogenetic analysis using betasatellites formed a close cluster with Tomato yellow leaf curl betasatellites infecting tomato and Corchorus crops, which has already been reported to cause yellow vein mosaic and leaf curl disease in many cultivated and weed crops growing in Saudi Arabia. The identified begomovirus associated with yellow vein mosaic disease in mentha could be a mutated strain of TYLCV and tentatively designated as TYLCV-Mentha isolate. Based on published data and latest information, this is the first report of identification of Tomato yellow leaf

Digital Printing Quality Detection and Analysis Technology Based on CCD

Science.gov (United States)

He, Ming; Zheng, Liping

2017-12-01

With the help of CCD digital printing quality detection and analysis technology, it can carry out rapid evaluation and objective detection of printing quality, and can play a certain control effect on printing quality. It can be said CDD digital printing quality testing and analysis of the rational application of technology, its digital printing and printing materials for a variety of printing equipments to improve the quality of a very positive role. In this paper, we do an in-depth study and discussion based on the CCD digital print quality testing and analysis technology.

JERS-1 Synthetic Aperture Radar, 1- km Mosaic, Amazon Basin: 1995-1996

Data.gov (United States)

National Aeronautics and Space Administration — ABSTRACT: This data set contains two image mosaics of L-band radar backscatter and two image mosaics of first order texture. The two backscatter images are mosaics...

Experience in CCD Photometry at the Tartu Observatory

Directory of Open Access Journals (Sweden)

Tuvikene T.

2003-12-01

Full Text Available We give overview of the CCD instrumentation and data reduction techniques used at the Tartu Observatory. The first results from photometric observations of the peculiar variable V838 Mon are presented.

A New Waveform Mosaic Algorithm in the Vectorization of Paper Seismograms

Directory of Open Access Journals (Sweden)

Maofa Wang

2014-11-01

Full Text Available History paper seismograms are very important information for earthquake monitoring and prediction, and the vectorization of paper seismograms is a very import problem to be resolved. In this paper, a new waveform mosaic algorithm in the vectorization of paper seismograms is presented. We also give out the technological process to waveform mosaic, and a waveform mosaic system used to vectorize analog seismic record has been accomplished independently. Using it, we can precisely and speedy accomplish waveform mosaic for vectorizing analog seismic records.

Electromagnetic Compatibility Assessment of CCD Detector Acquisition Chains not Synchronized

Science.gov (United States)

Nicoletto, M.; Boschetti, D.; Ciancetta, E.; Maiorano, E.; Stagnaro, L.

2016-05-01

Euclid is a space observatory managed by the European Space Agency; it is the second medium class mission (see Figure 1) in the frame of Cosmic Vision 2015-2025 program.In the frame of this project, the electromagnetic interference between two different and not synchronized Charge Coupled Device (CCD) (see Figure 2) acquisition chains has been evaluated. The key parameter used for this assessment is the electromagnetic noise induced on each other. Taking into account the specificity of the issue, radiation coupling at relative low frequency and in near field conditions, classical approach based on simulations and testing on qualification model cannot be directly applied. Based on that, it has been decided to investigate the issue by test in an incremental way.

A Case Study of On-the-fly Wide-field Radio Imaging Applied to the Gravitational Wave Event GW151226

Science.gov (United States)

Mooley, K. P.; Frail, D. A.; Myers, S. T.; Kulkarni, S. R.; Hotokezaka, K.; Singer, L. P.; Horesh, A.; Kasliwal, M. M.; Cenko, S. B.; Hallinan, G.

2018-04-01

We apply a newly developed on-the-fly mosaicing technique on the Jansky Very Large Array (VLA) at 3 GHz in order to carry out a sensitive search for an afterglow from the Advanced LIGO binary black hole merger event GW151226. In three epochs between 1.5 and 6 months post-merger, we observed a 100 deg2 region, with more than 80% of the survey region having an rms sensitivity of better than 150 μJy/beam, in the northern hemisphere with a merger containment probability of 10%. The data were processed in near real time and analyzed to search for transients and variables. No transients were found but we have demonstrated the ability to conduct blind searches in a time-frequency phase space where the predicted afterglow signals are strongest. If the gravitational wave event is contained within our survey region, the upper limit on any late-time radio afterglow from the merger event at an assumed mean distance of 440 Mpc is about 1029 erg s‑1 Hz‑1. Approximately 1.5% of the radio sources in the field showed variability at a level of 30%, and can be attributed to normal activity from active galactic nuclei. The low rate of false positives in the radio sky suggests that wide-field imaging searches at a few Gigahertz can be an efficient and competitive search strategy. We discuss our search method in the context of the recent afterglow detection from GW170817 and radio follow-up in future gravitational wave observing runs.

A GRAPH READER USING A CCD IMAGE SENSOR

African Journals Online (AJOL)

2008-01-18

Jan 18, 2008 ... using a stepper motor controlled by a software program in a ... Keywords: CCD sensor, microcontrollen stepper motor and microcomputer. 1. ... commercial applications (Awcock and ... on-chip amplifier, one pixel at a tirtjie.

Design drivers for a wide-field multi-object spectrograph for the William Herschel Telescope

NARCIS (Netherlands)

Balcells, Marc; Benn, Chris R.; Carter, David; Dalton, Gavin B.; Trager, Scott C.; Feltzing, Sofia; Verheijen, M.A.W.; Jarvis, Matt; Percival, Will; Abrams, Don C.; Agocs, Tibor; Brown, Anthony G. A.; Cano, Diego; Evans, Chris; Helmi, Amina; Lewis, Ian J.; McLure, Ross; Peletier, Reynier F.; Pérez-Fournon, Ismael; Sharples, Ray M.; Tosh, Ian A. J.; Trujillo, Ignacio; Walton, Nic; Westhall, Kyle B.

Wide-field multi-object spectroscopy is a high priority for European astronomy over the next decade. Most 8-10m telescopes have a small field of view, making 4-m class telescopes a particularly attractive option for wide-field instruments. We present a science case and design drivers for a

C.C.D. readout of a picosecond streak camera with an intensified C.C.D

International Nuclear Information System (INIS)

Lemonier, M.; Richard, J.C.; Cavailler, C.; Mens, A.; Raze, G.

1984-08-01

This paper deals with a digital streak camera readout device. The device consists in a low light level television camera made of a solid state C.C.D. array coupled to an image intensifier associated to a video-digitizer coupled to a micro-computer system. The streak camera images are picked-up as a video signal, digitized and stored. This system allows the fast recording and the automatic processing of the data provided by the streak tube

EVALUATION OF RATIONAL FUNCTION MODEL FOR GEOMETRIC MODELING OF CHANG'E-1 CCD IMAGES

Directory of Open Access Journals (Sweden)

Y. Liu

2012-08-01

Full Text Available Rational Function Model (RFM is a generic geometric model that has been widely used in geometric processing of high-resolution earth-observation satellite images, due to its generality and excellent capability of fitting complex rigorous sensor models. In this paper, the feasibility and precision of RFM for geometric modeling of China's Chang'E-1 (CE-1 lunar orbiter images is presented. The RFM parameters of forward-, nadir- and backward-looking CE-1 images are generated though least squares solution using virtual control points derived from the rigorous sensor model. The precision of the RFM is evaluated by comparing with the rigorous sensor model in both image space and object space. Experimental results using nine images from three orbits show that RFM can precisely fit the rigorous sensor model of CE-1 CCD images with a RMS residual error of 1/100 pixel level in image space and less than 5 meters in object space. This indicates that it is feasible to use RFM to describe the imaging geometry of CE-1 CCD images and spacecraft position and orientation. RFM will enable planetary data centers to have an option to supply RFM parameters of orbital images while keeping the original orbit trajectory data confidential.

Self-standing quasi-mosaic crystals for focusing hard X-rays

International Nuclear Information System (INIS)

Camattari, Riccardo; Guidi, Vincenzo; Bellucci, Valerio; Neri, Ilaria; Frontera, Filippo; Jentschel, Michael

2013-01-01

A quasi mosaic bent crystal for high-resolution diffraction of X and γ rays has been realized. A net curvature was imprinted to the crystal thanks to a series of superficial grooves to keep the curvature without external devices. The crystal highlights very high diffraction efficiency due to quasi mosaic curvature. Quasi mosaic crystals of this kind are proposed for the realization of a high-resolution focusing Laue lens for hard X-rays.

Influence of Fire Mosaics, Habitat Characteristics and Cattle Disturbance on Mammals in Fire-Prone Savanna Landscapes of the Northern Kimberley.

Science.gov (United States)

Radford, Ian J; Gibson, Lesley A; Corey, Ben; Carnes, Karin; Fairman, Richard

2015-01-01

Patch mosaic burning, in which fire is used to produce a mosaic of habitat patches representative of a range of fire histories ('pyrodiversity'), has been widely advocated to promote greater biodiversity. However, the details of desired fire mosaics for prescribed burning programs are often unspecified. Threatened small to medium-sized mammals (35 g to 5.5 kg) in the fire-prone tropical savannas of Australia appear to be particularly fire-sensitive. Consequently, a clear understanding of which properties of fire mosaics are most instrumental in influencing savanna mammal populations is critical. Here we use mammal capture data, remotely sensed fire information (i.e. time since last fire, fire frequency, frequency of late dry season fires, diversity of post-fire ages in 3 km radius, and spatial extent of recently burnt, intermediate and long unburnt habitat) and structural habitat attributes (including an index of cattle disturbance) to examine which characteristics of fire mosaics most influence mammals in the north-west Kimberley. We used general linear models to examine the relationship between fire mosaic and habitat attributes on total mammal abundance and richness, and the abundance of the most commonly detected species. Strong negative associations of mammal abundance and richness with frequency of late dry season fires, the spatial extent of recently burnt habitat (post-fire age fire age classes in the models. Our results indicate that both a high frequency of intense late dry season fires and extensive, recently burnt vegetation are likely to be detrimental to mammals in the north Kimberley. A managed fire mosaic that reduces large scale and intense fires, including the retention of ≥4 years unburnt patches, will clearly benefit savanna mammals. We also highlighted the importance of fire mosaics that retain sufficient shelter for mammals. Along with fire, it is clear that grazing by introduced herbivores also needs to be reduced so that habitat quality is

Chromosomal mosaicism in human preimplantation embryos: a systematic review.

NARCIS (Netherlands)

Echten-Arends, J. van; Mastenbroek, S.; Sikkema-Raddatz, B.; Korevaar, J.C.; Heineman, M.J.; Veen, F. van der; Repping, S.

2011-01-01

BACKGROUND: Although chromosomal mosaicism in human preimplantation embryos has been described for almost two decades, its exact prevalence is still unknown. The prevalence of mosaicism is important in the context of preimplantation genetic screening in which the chromosomal status of an embryo is

Chromosomal mosaicism in human preimplantation embryos : a systematic review

NARCIS (Netherlands)

van Echten-Arends, Jannie; Mastenbroek, Sebastiaan; Sikkema-Raddatz, Birgit; Korevaar, Johanna C.; Heineman, Maas Jan; van der Veen, Fulco; Repping, Sjoerd

2011-01-01

BACKGROUND: Although chromosomal mosaicism in human preimplantation embryos has been described for almost two decades, its exact prevalence is still unknown. The prevalence of mosaicism is important in the context of preimplantation genetic screening in which the chromosomal status of an embryo is

Deep CCD survey - galaxy luminosity and color evolution

International Nuclear Information System (INIS)

Tyson, J.A.

1988-01-01

Imaging and photometric observations of a statistically complete sample of galaxies in 12 high-latitude fields, obtained in the BJ (360-520 nm), R (580-720 nm) and I (780-1100 nm) bands using CCD detectors on the 4-m telescopes at CTIO and KPNO, are reported. The data are presented in extensive graphs and sample images and analyzed in detail with reference to theoretical models of galactic origin and evolution. The galaxy number-count slopes, d(log N)/dm, are found to be sub-Euclidean, varying from 0.34 in the I band to 0.45 in the BJ band, where the corrected counts appear to saturate at about 27 mag. The predictions of no-evolution models are shown to underpredict the counts at 25 mag (BJ) by a factor of 5-15 and the extragalactic background light from all galaxies (6.8 x 10 to the -6th erg/sq cm sec sr micron at 450 nm) by a factor greater than 2. 114 references

Mosaic boreal landscapes with open and forested wetlands

International Nuclear Information System (INIS)

Sjoeberg, K.; Ericson, L.

1997-01-01

We review patterns and processes important for biodiversity in the Fennoscandian boreal forest, describe man's past and present impact and outline a strategy for conservation. The boreal landscape was earlier characterized by a mosaic of open and forested wetlands and forests. Drainage and felling operation have largely changed that pattern. Several organisms depend upon the landscape mosaic. Natural ecotones between mire and forest provide food resources predictable in space and time contrasting to unpredictable edges in the silvicultured landscape. The mosaic is also a prerequisite for organisms dependent on non-substitutable resources in the landscape. The importance of swamp forests has increased as they function as refugia for earlier more widespread old-growth species. Programmes for maintaining biodiversity in the boreal landscape should include the following points. First, the natural mosaic with open and forested wetlands must be maintained. Second, swamp forests must receive a general protection as they often constitute the only old-growth patches in the landscape. Third, we need to restore earlier disturbance regimes. Present strategy plans for conservation are insufficient, as they imply that a too large proportion of boreal organisms will not be able to survive outside protected areas. Instead, we need to focus more on how to preserve organisms in the man-influenced landscape. As a first step we need to understand how organisms are distributed in landscapes at various spatial scales. We need studies in landscapes where the original mosaic has faced various degrees of fragmentation. (au) 124 refs

Challenges and advantages in wide-field optical coherence tomography angiography imaging of the human retinal and choroidal vasculature at 1.7-MHz A-scan rate

Science.gov (United States)

Poddar, Raju; Migacz, Justin V.; Schwartz, Daniel M.; Werner, John S.; Gorczynska, Iwona

2017-10-01

We present noninvasive, three-dimensional, depth-resolved imaging of human retinal and choroidal blood circulation with a swept-source optical coherence tomography (OCT) system at 1065-nm center wavelength. Motion contrast OCT imaging was performed with the phase-variance OCT angiography method. A Fourier-domain mode-locked light source was used to enable an imaging rate of 1.7 MHz. We experimentally demonstrate the challenges and advantages of wide-field OCT angiography (OCTA). In the discussion, we consider acquisition time, scanning area, scanning density, and their influence on visualization of selected features of the retinal and choroidal vascular networks. The OCTA imaging was performed with a field of view of 16 deg (5 mm×5 mm) and 30 deg (9 mm×9 mm). Data were presented in en face projections generated from single volumes and in en face projection mosaics generated from up to 4 datasets. OCTA imaging at 1.7 MHz A-scan rate was compared with results obtained from a commercial OCTA instrument and with conventional ophthalmic diagnostic methods: fundus photography, fluorescein, and indocyanine green angiography. Comparison of images obtained from all methods is demonstrated using the same eye of a healthy volunteer. For example, imaging of retinal pathology is presented in three cases of advanced age-related macular degeneration.

Design of wide flat-topped low transverse field solenoid magnet

International Nuclear Information System (INIS)

Jing Xiaobing; Chen Nan; Li Qin

2010-01-01

A wide flat-topped low transverse error field solenoid magnet design for linear induction accelerator is presented. The design features non-uniform winding to reduce field fluctuation due to the magnets' gap, and homogenizer rings within the solenoid to greatly reduce the effects of winding errors. Numerical modeling of several designs for 12 MeV linear induction accelerator (LIA) in China Academy of Engineering Physics has demonstrated that by using these two techniques the magnetic field fluctuations in the accelerator gap can be reduced by 70% and the transverse error field can be reduced by 96.5%. (authors)

Protein diffraction experiments with Atlas CCD detector

Czech Academy of Sciences Publication Activity Database

Dohnálek, Jan; Kovaľ, Tomáš; Dušek, Michal

2008-01-01

Roč. 64, Suppl. - abstracts (2008), C192 ISSN 0108-7673. [Congress of the International Union of Crystallography (IUCr) /21./. 23.08.2008-31.08.2008, Osaka] Institutional research plan: CEZ:AV0Z10100521 Keywords : x-ray data collection * CCD detectors * protein crystallography applications Subject RIV: BM - Solid Matter Physics ; Magnetism

TOGA COARE Satellite data summaries available on the World Wide Web

Science.gov (United States)

Chen, S. S.; Houze, R. A., Jr.; Mapes, B. E.; Brodzick, S. R.; Yutler, S. E.

1995-01-01

Satellite data summary images and analysis plots from the Tropical Ocean Global Atmosphere Coupled Ocean-Atmosphere Response Experiment (TOGA COARE), which were initially prepared in the field at the Honiara Operations Center, are now available on the Internet via World Wide Web browsers such as Mosaic. These satellite data summaries consist of products derived from the Japanese Geosynchronous Meteorological Satellite IR data: a time-size series of the distribution of contiguous cold cloudiness areas, weekly percent high cloudiness (PHC) maps, and a five-month time-longitudinal diagram illustrating the zonal motion of large areas of cold cloudiness. The weekly PHC maps are overlaid with weekly mean 850-hPa wind calculated from the European Centre for Medium-Range Weather Forecasts (ECMWF) global analysis field and can be viewed as an animation loop. These satellite summaries provide an overview of spatial and temporal variabilities of the cloud population and a large-scale context for studies concerning specific processes of various components of TOGA COARE.

Discussion on the fusing methods for HR and CCD images of CBERS

International Nuclear Information System (INIS)

Gao Zhangsheng; Zhao Yingjun

2010-01-01

CBERS-02B multi-spectral CCD data are different from HR panchromatic data in resolution, which causes difficulty in image fusion. With the method of Pansharping, HPF, Brovey transform, IHS transform, principal component transform, Gram Schmidt (GS) transform and wavelet transform, the authors have tested the fusion methods for CCD data and HR data of CBERS, and the fusion results are discussed and evaluated qualitatively and quantitatively. (authors)

Incidence of cassava mosaic disease and associated whitefly vectors in South West and North Central Nigeria: Data exploration.

Science.gov (United States)

Eni, Angela O; Efekemo, Oghenevwairhe P; Soluade, Mojisola G; Popoola, Segun I; Atayero, Aderemi A

2018-08-01

Cassava mosaic disease (CMD) is one of the most economically important viral diseases of cassava, an important staple food for over 800 million people in the tropics. Although several Cassava mosaic virus species associated with CMD have been isolated and characterized over the years, several new super virulent strains of these viruses have evolved due to genetic recombination between diverse species. In this data article, field survey data collected from 184 cassava farms in 12 South Western and North Central States of Nigeria in 2015 are presented and extensively explored. In each State, one cassava farm was randomly selected as the first farm and subsequent farms were selected at 10 km intervals, except in locations were cassava farms are sporadically located. In each selected farm, 30 cassava plants were sampled along two diagonals and all selected plant was scored for the presence or absence of CMD symptoms. Cassava mosaic disease incidence and associated whitefly vectors in South West and North Central Nigeria are explored using relevant descriptive statistics, box plots, bar charts, line graphs, and pie charts. In addition, correlation analysis, Analysis of Variance (ANOVA), and multiple comparison post-hoc tests are performed to understand the relationship between the numbers of whiteflies counted, uninfected farms, infected farms, and the mean of symptom severity in and across the States under investigation. The data exploration provided in this data article is considered adequate for objective assessment of the incidence and symptom severity of cassava mosaic disease and associated whitefly vectors in farmers' fields in these parts of Nigeria where cassava is heavily cultivated.

Mitigating fluorescence spectral overlap in wide-field endoscopic imaging

Science.gov (United States)

Hou, Vivian; Nelson, Leonard Y.; Seibel, Eric J.

2013-01-01

Abstract. The number of molecular species suitable for multispectral fluorescence imaging is limited due to the overlap of the emission spectra of indicator fluorophores, e.g., dyes and nanoparticles. To remove fluorophore emission cross-talk in wide-field multispectral fluorescence molecular imaging, we evaluate three different solutions: (1) image stitching, (2) concurrent imaging with cross-talk ratio subtraction algorithm, and (3) frame-sequential imaging. A phantom with fluorophore emission cross-talk is fabricated, and a 1.2-mm ultrathin scanning fiber endoscope (SFE) is used to test and compare these approaches. Results show that fluorophore emission cross-talk could be successfully avoided or significantly reduced. Near term, the concurrent imaging method of wide-field multispectral fluorescence SFE is viable for early stage cancer detection and localization in vivo. Furthermore, a means to enhance exogenous fluorescence target-to-background ratio by the reduction of tissue autofluorescence background is demonstrated. PMID:23966226

Wide field-of-view dual-band multispectral muzzle flash detection

Science.gov (United States)

Montoya, J.; Melchor, J.; Spiliotis, P.; Taplin, L.

2013-06-01

Sensor technologies are undergoing revolutionary advances, as seen in the rapid growth of multispectral methodologies. Increases in spatial, spectral, and temporal resolution, and in breadth of spectral coverage, render feasible sensors that function with unprecedented performance. A system was developed that addresses many of the key hardware requirements for a practical dual-band multispectral acquisition system, including wide field of view and spectral/temporal shift between dual bands. The system was designed using a novel dichroic beam splitter and dual band-pass filter configuration that creates two side-by-side images of a scene on a single sensor. A high-speed CMOS sensor was used to simultaneously capture data from the entire scene in both spectral bands using a short focal-length lens that provided a wide field-of-view. The beam-splitter components were arranged such that the two images were maintained in optical alignment and real-time intra-band processing could be carried out using only simple arithmetic on the image halves. An experiment related to limitations of the system to address multispectral detection requirements was performed. This characterized the system's low spectral variation across its wide field of view. This paper provides lessons learned on the general limitation of key hardware components required for multispectral muzzle flash detection, using the system as a hardware example combined with simulated multispectral muzzle flash and background signatures.

Advanced microlens and color filter process technology for the high-efficiency CMOS and CCD image sensors

Science.gov (United States)

Fan, Yang-Tung; Peng, Chiou-Shian; Chu, Cheng-Yu

2000-12-01

New markets are emerging for digital electronic image device, especially in visual communications, PC camera, mobile/cell phone, security system, toys, vehicle image system and computer peripherals for document capture. To enable one-chip image system that image sensor is with a full digital interface, can make image capture devices in our daily lives. Adding a color filter to such image sensor in a pattern of mosaics pixel or wide stripes can make image more real and colorful. We can say 'color filter makes the life more colorful color filter is? Color filter means can filter image light source except the color with specific wavelength and transmittance that is same as color filter itself. Color filter process is coating and patterning green, red and blue (or cyan, magenta and yellow) mosaic resists onto matched pixel in image sensing array pixels. According to the signal caught from each pixel, we can figure out the environment image picture. Widely use of digital electronic camera and multimedia applications today makes the feature of color filter becoming bright. Although it has challenge but it is very worthy to develop the process of color filter. We provide the best service on shorter cycle time, excellent color quality, high and stable yield. The key issues of advanced color process have to be solved and implemented are planarization and micro-lens technology. Lost of key points of color filter process technology have to consider will also be described in this paper.

Predation determines different selective pressure on pea aphid host races in a complex agricultural mosaic.

Directory of Open Access Journals (Sweden)

Adalbert Balog

Full Text Available Field assessments were conducted to examine the interplay between host plant and predation in complex agricultural mosaic on pea aphid clover and alfalfa races. In one experiment, we examined the relative fitness on clover race (CR and alfalfa race (AR pea aphids on broad bean, red clover and alfalfa alone. But because clover is typically grown in a more complex agricultural mosaic with alfalfa and broad bean, a second experiment was conducted to assess the fitness consequences under predation in a more complex agricultural field setting that also included potential apparent competition with AR pea aphids. In a third experiment we tested for the effect of differential host race density on the fitness of the other host race mediated by a predator effect. CR pea aphids always had fitness losses when on broad bean (had lower fitness on broad bean relative to red clover and fitness benefits when on red clover (higher fitness on red clover relative to broad bean, whether or not in apparent competition with alfalfa race aphids on bean and alfalfa. AR suffered fitness loss on both alfalfa and bean in apparent competition with CR on clover. Therefore we can conclude that the predation rate between host races was highly asymmetrical. The complexity of the agricultural mosaic thus can influence prey selection by predators on different host plants. These may have evolutionary consequences through context dependent fitness benefits on particular host plants.

Entirely saturated unilateral smear of laser spot in CCD

International Nuclear Information System (INIS)

Zhang Zhen; Zhou Menglian; Zhang Jianmin; Lin Xinwei

2013-01-01

In the video of linear CCD camera being irradiated by 532 nm CW laser, the entirely saturated unilateral smear of laser spot was found. The smear area does not represent the distribution of laser. Since this smear lies merely in one side of laser spot, it can not be induced by light leaking or carriers blooming, and it may be induced by charge transfer loss. However, the feature that the smear area is entirely saturated can not be explained by the current constant model of charge transfer inefficiency. Based on the inner structure and operating principle of buried channel CCD, a new model of charge transfer inefficiency that varies with charge quantity is proposed, which can explain the entirely saturated unilateral smear of laser spot. (authors)

Retro reflective glass mosaic; Mosaico Vitreo Retrorreflectante

Energy Technology Data Exchange (ETDEWEB)

Belda, A.; Orts, M. J.; Viciano, F.; Lucas, F.

2012-07-01

Salquisa and Alttoglass have developed a very innovative product : the retro reflective glass mosaic. This new product can be used in both horizontal and vertical signposting and also in interior design and architecture. This particular product has many advantages compare to the traditional methods used for signposting, design or architecture. One of them is that the product is mainly made of glass therefore it can last much longer than paints for example. The used of glass mosaic for civil engineering it is opened up especially for signposting and it contributes to improve visibility at night not only in standard conditions but also in the hard ones such as wind, fog or rain at nighttimes. Higher visibility = higher security. We should remember that a high percentage of accidents occur under rain conditions at night. The glass mosaic is presented in a mesh which allows the use in both plane and curve surfaces in signposting, interior design and architecture. The retro reflective effect last under the water therefore the mosaic can be fixed in ornamental and decorative fountains, swimming pools, etc. Furthermore, the retro reflective effect can also be applied on big size ceramic tiles. This project was developed along with the Institute of Ceramic Technology (ITC), it was supported by the Center for Industrial Technological Development (CDTI) and it is also patented. (Author)

WATERMELON MOSAIC VIRUS OF PUMPKIN (Cucurbita maxima) FROM SULAWESI: IDENTIFICATION, TRANSMISSION, AND HOST RANGE

OpenAIRE

Wasmo Wakmana; M.S. Kontong; D.S. Teakle; D.M. Persley

2016-01-01

A mosaic disease of pumpkin (Cucurbita maxima) was spread widely in Sulawesi. Since the virus had not yet been identified, a study was conducted to identify the disease through mechanical inoculation, aphid vector transmission, host range, and electron microscopic test. Crude sap of infected pumpkin leaf samples was rubbed on the cotyledons of healthy pumpkin seedlings for mechanical inoculation. For insect transmission, five infective aphids were infected per seedling. Seedlings of eleven di...

Watermelon Mosaic Virus Of Pumpkin (Cucurbita Maxima) From Sulawesi: Identification, Transmission, And Host Range

OpenAIRE

Wakmana, Wasmo; Kontong, M.S; Teakle, D.S; Persley, D.M

2002-01-01

A mosaic disease of pumpkin (Cucurbita maxima) was spread widely in Sulawesi. Since the virus had not yet been identified, a study was conducted to identify the disease through mechanical inoculation, aphid vector transmission, host range, and electron microscopic test. Crude sap of infected pumpkin leaf samples was rubbed on the cotyledons of healthy pumpkin seedlings for mechanical inoculation. For insect transmission, five infective aphids were infected per seedling. Seedlings of eleven di...

CCD developed for scientific application by Hamamatsu

CERN Document Server

Miyaguchi, K; Dezaki, J; Yamamoto, K

1999-01-01

We have developed CCDs for scientific applications that feature a low readout noise of less than 5 e-rms and low dark current of 10-25 pA/cm sup 2 at room temperature. CCDs with these characteristics will prove extremely useful in applications such as spectroscopic measurement and dental radiography. In addition, a large-area CCD of 2kx4k pixels and 15 mu m square pixel size has recently been completed for optical use in astronomical observations. Applications to X-ray astronomy require the most challenging device performance in terms of deep depletion, high CTE, and focal plane size, among others. An abuttable X-ray CCD, having 1024x1024 pixels and 24 mu m square pixel size, is to be installed in an international space station (ISS). We are now striving to achieve the lowest usable cooling temperature by means of a built-in TEC with limited power consumption. Details on the development status are described in this report. We would also like to present our future plans for a large active area and deep depleti...

High performance CCD camera system for digitalisation of 2D DIGE gels.

Science.gov (United States)

Strijkstra, Annemieke; Trautwein, Kathleen; Roesler, Stefan; Feenders, Christoph; Danzer, Daniel; Riemenschneider, Udo; Blasius, Bernd; Rabus, Ralf

2016-07-01

An essential step in 2D DIGE-based analysis of differential proteome profiles is the accurate and sensitive digitalisation of 2D DIGE gels. The performance progress of commercially available charge-coupled device (CCD) camera-based systems combined with light emitting diodes (LED) opens up a new possibility for this type of digitalisation. Here, we assessed the performance of a CCD camera system (Intas Advanced 2D Imager) as alternative to a traditionally employed, high-end laser scanner system (Typhoon 9400) for digitalisation of differential protein profiles from three different environmental bacteria. Overall, the performance of the CCD camera system was comparable to the laser scanner, as evident from very similar protein abundance changes (irrespective of spot position and volume), as well as from linear range and limit of detection. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

First report of Apple necrotic mosaic virus infecting apple trees in Korea

Science.gov (United States)

In September 2016, two apple trees (Malus domestica Borkh) cv. Shinano Sweet showing bright cream spot and mosaic patterns on leaves were observed in Pocheon, South Korea. Mosaic symptoms are common on leaves of apple trees infected with Apple mosaic virus (ApMV). Symptomatic leaves were tested by e...

Technical challenges and recent progress in CCD imagers

International Nuclear Information System (INIS)

Bosiers, Jan T.; Peters, Inge M.; Draijer, Cees; Theuwissen, Albert

2006-01-01

This paper gives a review of the performance of charge-coupled device (CCD) imagers for use in consumer, professional and scientific applications. An overview of recent developments and the current state-of-the-art are presented. An extensive list of references is included

BEAM-ish: A Graphical User Interface for the Physical Characterization of Macromolecular Crystals

Science.gov (United States)

Lovelace, Jeff; Snell, Edward H.; Pokross, Matthew; Arvai, Andrew S.; Nielsen, Chris; Nguyen, Xuong; Bellamy, Henry D; Borgstahl, Gloria E. O.; Rose, M. Franklin (Technical Monitor)

2000-01-01

Crystal mosaicity is determined from the measurement of the reflection angular width and can be used as an indicator of crystal perfection. A new method has been developed that combines the use of unfocused synchrotron radiation, super-fine phi slicing and a CCD area detector to simultaneously measure the mosaicity of hundreds of reflections . The X-ray beam characteristics and Lorentz correction are deconvoluted from the resulting reflection widths to calculate the true crystal mosaicity.

CCD [charge-coupled device] sensors in synchrotron x-ray detectors

International Nuclear Information System (INIS)

Strauss, M.G.; Naday, I.; Sherman, I.S.; Kraimer, M.R.; Westbrook, E.M.; Zaluzec, N.J.

1987-01-01

The intense photon flux from advanced synchrotron light sources, such as the 7-GeV synchrotron being designed at Argonne, require integrating-type detectors. Charge-coupled devices (CCDs) are well suited as synchrotron x-ray detectors. When irradiated indirectly via a phosphor followed by reducing optics, diffraction patterns of 100 cm 2 can be imaged on a 2 cm 2 CCD. With a conversion efficiency of ∼1 CCD electron/x-ray photon, a peak saturation capacity of >10 6 x rays can be obtained. A programmable CCD controller operating at a clock frequency of 20 MHz has been developed. The readout rate is 5 x 10 6 pixels/s and the shift rate in the parallel registers is 10 6 lines/s. The test detector was evaluated in two experiments. In protein crystallography diffraction patterns have been obtained from a lysozyme crystal using a conventional rotating anode x-ray generator. Based on these results we expect to obtain at a synchrotron diffraction images at the rate of ∼1 frame/s or a complete 3-dimensional data set from a single crystal in ∼2 min. 16 refs., 16 figs., 2 tabs

High-performance visible/UV CCD focal plane technology for spacebased applications

Science.gov (United States)

Burke, B. E.; Mountain, R. W.; Gregory, J. A.; Huang, J. C. M.; Cooper, M. J.; Savoye, E. D.; Kosicki, B. B.

1993-01-01

We describe recent technology developments aimed at large CCD imagers for space based applications in the visible and UV. Some of the principal areas of effort include work on reducing device degradation in the natural space-radiation environment, improvements in quantum efficiency in the visible and UV, and larger-device formats. One of the most serious hazards for space based CCD's operating at low signal levels is the displacement damage resulting from bombardment by energetic protons. Such damage degrades charge-transfer efficiency and increases dark current. We have achieved improved hardness to proton-induced displacement damage by selective ion implants into the CCD channel and by reduced temperature of operation. To attain high quantum efficiency across the visible and UV we have developed a technology for back-illuminated CCD's. With suitable antireflection (AR) coatings such devices have quantum efficiencies near 90 percent in the 500-700-nm band. In the UV band from 200 to 400 nm, where it is difficult to find coatings that are sufficiently transparent and can provide good matching to the high refractive index of silicon, we have been able to substantially increase the quantum efficiency using a thin film of HfO2 as an AR coating. These technology efforts were applied to a 420 x 420-pixel frame-transfer imager, and future work will be extended to a 1024 x 1024-pixel device now under development.

Multi-spectral CCD camera system for ocean water color and seacoast observation

Science.gov (United States)

Zhu, Min; Chen, Shiping; Wu, Yanlin; Huang, Qiaolin; Jin, Weiqi

2001-10-01

One of the earth observing instruments on HY-1 Satellite which will be launched in 2001, the multi-spectral CCD camera system, is developed by Beijing Institute of Space Mechanics & Electricity (BISME), Chinese Academy of Space Technology (CAST). In 798 km orbit, the system can provide images with 250 m ground resolution and a swath of 500 km. It is mainly used for coast zone dynamic mapping and oceanic watercolor monitoring, which include the pollution of offshore and coast zone, plant cover, watercolor, ice, terrain underwater, suspended sediment, mudflat, soil and vapor gross. The multi- spectral camera system is composed of four monocolor CCD cameras, which are line array-based, 'push-broom' scanning cameras, and responding for four spectral bands. The camera system adapts view field registration; that is, each camera scans the same region at the same moment. Each of them contains optics, focal plane assembly, electrical circuit, installation structure, calibration system, thermal control and so on. The primary features on the camera system are: (1) Offset of the central wavelength is better than 5 nm; (2) Degree of polarization is less than 0.5%; (3) Signal-to-noise ratio is about 1000; (4) Dynamic range is better than 2000:1; (5) Registration precision is better than 0.3 pixel; (6) Quantization value is 12 bit.

Intensified CCD for ultrafast diagnostics

International Nuclear Information System (INIS)

Cheng, J.; Tripp, G.; Coleman, L.

1978-01-01

Many of the present laser fusion diagnostics are recorded on either ultrafast streak cameras or on oscilloscopes. For those experiments in which a large volume of data is accumulated, direct computer processing of the information becomes important. We describe an approach which uses a RCA 52501 back-thinned CCD sensor to obtain direct electron readouts for both the streak camera and the CRT. Performance of the 100 GHz streak camera and the 4 GHz CRT are presented. Design parameters and computer interfacing for both systems are described in detail

Sugarcane Elongin C is involved in infection by sugarcane mosaic disease pathogens.

Science.gov (United States)

Zhai, Yushan; Deng, Yuqing; Cheng, Guangyuan; Peng, Lei; Zheng, Yanru; Yang, Yongqing; Xu, Jingsheng

2015-10-23

Sugarcane (Saccharum sp. hybrid) provides the main source of sugar for humans. Sugarcane mosaic disease (SMD) is a major threat to sugarcane production. Currently, control of SMD is mainly dependent on breeding resistant cultivars through hybridization, which is time-consuming. Understanding the mechanism of viral infection may facilitate novel strategies to breed cultivars resistant to SMD and to control the disease. In this study, a wide interaction was detected between the viral VPg protein and host proteins. Several genes were screened from sugarcane cDNA library that could interact with Sugarcane streak mosaic virus VPg, including SceIF4E1 and ScELC. ScELC was predicted to be a cytoplasmic protein, but subcellular localization analysis showed it was distributed both in cytoplasmic and nuclear, and interactions were also detected between ScELC and VPg of SCMV or SrMV that reveal ScELC was widely used in the SMD pathogen infection process. ScELC and VPgs interacted in the nucleus, and may function to enhance the viral transcription rate. ScELC also interacted with SceIF4E2 both in the cytoplasm and nucleus, but not with SceIF4E1 and SceIF4E3. These results suggest that ScELC may be essential for the function of SceIF4E2, an isomer of eIF4E. Copyright © 2015 Elsevier Inc. All rights reserved.

The research of digital circuit system for high accuracy CCD of portable Raman spectrometer

Science.gov (United States)

Yin, Yu; Cui, Yongsheng; Zhang, Xiuda; Yan, Huimin

2013-08-01

The Raman spectrum technology is widely used for it can identify various types of molecular structure and material. The portable Raman spectrometer has become a hot direction of the spectrometer development nowadays for its convenience in handheld operation and real-time detection which is superior to traditional Raman spectrometer with heavy weight and bulky size. But there is still a gap for its measurement sensitivity between portable and traditional devices. However, portable Raman Spectrometer with Shell-Isolated Nanoparticle-Enhanced Raman Spectroscopy (SHINERS) technology can enhance the Raman signal significantly by several orders of magnitude, giving consideration in both measurement sensitivity and mobility. This paper proposed a design and implementation of driver and digital circuit for high accuracy CCD sensor, which is core part of portable spectrometer. The main target of the whole design is to reduce the dark current generation rate and increase signal sensitivity during the long integration time, and in the weak signal environment. In this case, we use back-thinned CCD image sensor from Hamamatsu Corporation with high sensitivity, low noise and large dynamic range. In order to maximize this CCD sensor's performance and minimize the whole size of the device simultaneously to achieve the project indicators, we delicately designed a peripheral circuit for the CCD sensor. The design is mainly composed with multi-voltage circuit, sequential generation circuit, driving circuit and A/D transition parts. As the most important power supply circuit, the multi-voltage circuits with 12 independent voltages are designed with reference power supply IC and set to specified voltage value by the amplifier making up the low-pass filter, which allows the user to obtain a highly stable and accurate voltage with low noise. What's more, to make our design easy to debug, CPLD is selected to generate sequential signal. The A/D converter chip consists of a correlated

Reassessing Jacob Strauss and the Mosaic Code

Directory of Open Access Journals (Sweden)

Joel McDurmon

2012-01-01

Full Text Available This article reviewed claims made by modern scholars Ford Lewis Battles, G.H. Williams, and Theodore Tappert concerning the views of Jacob Strauss (1480–1530, court preacher at Eisenach, particularly in regard to the imposition of Mosaic Law upon the civil realm. Most pointedly, Battles claims Strauss proposed to replace European civil law completely with the ‘entire Mosaic code’. This study examined Strauss’s relevant writings to determine his position on Mosaic Law and civil law and demonstrated that the claims of Battles, Williams, and Tappert were not supported by the primary source evidence. Selections from Strauss’ 51 theses on usury are translated into English for the first time. To a much lesser degree, this study addressed the issue in regard to the Weimar court preacher Wolfgang Stein, against whom the same claims were made. A paucity of evidence rendered those claims dubious in his case. In the end we were left only with unsubstantiated second-hand claims against these men.

A MULTI-CORE PARALLEL MOSAIC ALORITHM FOR MULTI-VIEW UAV IMAGES

Directory of Open Access Journals (Sweden)

X. Pan

2017-09-01

Full Text Available As the spread of the error and accumulation often lead to distortion or failure of image mosaic during the multi-view UAV (Unmanned Aerial Vehicle images stitching. In this paper, to solve the problem we propose a mosaic strategy to construct a mosaic ring and multi-level grouping parallel acceleration as an auxiliary. First, the input images will be divided into several groups, each group in the ring way to stitch. Then, use SIFT for matching, RANSAC to remove the wrong matching points. And then, calculate the perspective transformation matrix. Finally weaken the error by using the adjustment equation. All these steps run between different groups at the same time. By using real UAV images, the experiment results show that this method can effectively reduce the influence of accumulative error, improve the precision of mosaic and reduce the mosaic time by 60 %. The proposed method can be used as one of the effective ways to minimize the accumulative error.

Rethinking CCD's Significance in Estimating Late Neogene Whole Ocean Carbonate Budget

Science.gov (United States)

Si, W.; Rosenthal, Y.

2017-12-01

The global averaged calcite compensation depth (CCD) record is conventionally used to reconstruct two correlatable parameters of the carbonate system - the alkalinity budget of the ocean and/or the saturation state of the ocean. Accordingly, the available CCD reconstructions have been interpreted to suggest either relative stable (Pearson and Palmer, 2000) or increased alkalinity of the ocean over the past 15 Ma (Tyrrell and Zeebe, 2004; Pälike et al., 2012). However, CCD alone is insufficient to constrain the carbonate system because the weathering flux of alkalinity into the ocean is not only balanced by CaCO3 dissolution on the seafloor but also by the biologic production in the euphotic zone and, the CCD records cannot be readily interpreted as changes in either process. Here, we present evidence of the co-evolution of surface CaCO3 production and deepsea dissolution through the late Neogene. By examining separately the mass accumulation rates (MAR) of coccoliths, planktonic foraminifera, and quantifying dissolution (using a proxy revised from Broecker et al., 1999) in seventeen deepsea cores from multiple depth-transects, we find that 1) MAR of dissolution-resistant coccoliths was substantially higher in the mid Miocene and declining on a global scale towards the present; 2) unlike coccoliths, MAR of planktonic foraminifera, shows no apparent secular trend through that time; 3) the revised dissolution index, shows significantly improved preservation of planktonic foraminiferal shells over that time, particularly at intermediate water depth and exhibits close association between changes in preservation with key climatic events. Our new records have two immediate implications. First, the substantially weakened pelagic biogenic carbonate production from mid Miocene to present alone could account for the improved preservation of deepsea carbonates without calling for a scenario of increased weathering input. Second, with the constrain of global averaged CCD

Mosaic Pattern of Lung Attenuation on Chest CT in Patients with Pulmonary Hypertension

Directory of Open Access Journals (Sweden)

Kamonpun Ussavarungsi

2015-09-01

Full Text Available A mosaic pattern of lung attenuation on chest computed tomography (CT may be due to various etiologies. There is limited published data on CT results when used to evaluate pulmonary hypertension (PH. We retrospectively studied the frequency of mosaic pattern in patients with PH and the cause of the PH by diagnostic group, as well as the correlation between the mosaic pattern and the following: demographics, severity of the PH, main pulmonary artery (PA size, PA/aorta (PA/Ao ratio, pulmonary function tests (PFT, and ventilation perfusion scan results. Overall, 18% of the cohort had CT mosaic pattern (34/189. Mosaic pattern was present in 17/113 (15% in Group 1 pulmonary arterial hypertension, 5/13 (28% in Group 2 pulmonary venous hypertension and 8/50 (16% in Group 3 PH. Conversely, Group 4 chronic thromboembolic PH was more prevalent in 4/8 (50%. Main PA size, PA/Ao ratio, and segmental perfusion defect were positively associated with mosaic pattern. In contrast, factors such as age, gender, body mass index, functional class, hemodynamic data, and PFT values were not associated with mosaic pattern. Mosaic pattern is not specific as an isolated finding for distinguishing the subtype of PH.

Identification of virus isolates inducing mosaic of sugarcane in ...

African Journals Online (AJOL)

SAM

2014-03-19

Mar 19, 2014 ... (JGMV), maize dwarf mosaic virus (MDMV) and sorghum mosaic Virus (SrMV) is an economically important viral disease of sugarcane ... race (“Bahausa”) and the least infected was the white land race (“fararkwama”). ... stripes symptoms on leaf blade and white stripe on stem in infected sugarcane and are ...

Identification and Molecular Analysis of Bean common mosaic virus (BCMV and Bean common mosaic necrosis virus (BCMNV in Mazandaran Province

Directory of Open Access Journals (Sweden)

Z. Moradi

2016-06-01

Full Text Available Introduction: Among legume crops, common bean (Phaseolus vulgaris L. is one of the most important worldwide crops, because of its cultivation area and nutritional value. The closely related potyviruses Bean common mosaic virus (BCMV and Bean common mosaic necrosis virus (BCMNV are the most common and most destructive viruses that infect common beans throughout the world. The viruses induced similar symptoms in numerous bean genotypes, including mosaic, leaf distortion, stunting, and lethal necrosis. Like all potyviruses, BCMV and BCMNV have non-enveloped flexuous filamentous virions of 750 nm long and 11–13 nm wide, which encapsidate a single-stranded, positive-sense RNA molecule of approximately 10,000 nt long. Both are naturally transmitted by aphids in a non-persistent manner and by seed, which explains their worldwide distribution. These viruses are major constraints on bean production and can cause serious crop losses. Mazanadaran province in north of Iran is one of the major producing areas of legumes, so identification of these viruses is a concern. However, so far, no studies have been done with these viruses in this province. The aim of this research was to study the existence of BCMV and BCMNV in research areas and determining of their phylogenetic relationship. Polymerase chain reaction (PCR with degenerate primers for conserved sequences of the viral genomes has facilitated the rapid detection of many potyviruses and enabled partial genomic sequencing. In the absence of complete genomic sequences of potyviruses, CI-coding region is more suitable for diagnostic and taxonomy purposes, rather than the coat protein (CP usually used. The CI gene most accurately reflects the taxonomic status according to the complete ORF. Materials and Methods: From July to September 2013 and 2014, a total of 50 leaf samples of beans showing virus symptoms were collected from different bean fields in Mazandaran province. Total RNA was extracted from all

Measurement of phase function of aerosol at different altitudes by CCD Lidar

Science.gov (United States)

Sun, Peiyu; Yuan, Ke'e.; Yang, Jie; Hu, Shunxing

2018-02-01

The aerosols near the ground are closely related to human health and climate change, the study on which has important significance. As we all know, the aerosol is inhomogeneous at different altitudes, of which the phase function is also different. In order to simplify the retrieval algorithm, it is usually assumed that the aerosol is uniform at different altitudes, which will bring measurement error. In this work, an experimental approach is demonstrated to measure the scattering phase function of atmospheric aerosol particles at different heights by CCD lidar system, which could solve the problem of the traditional CCD lidar system in assumption of phase function. The phase functions obtained by the new experimental approach are used to retrieve the aerosol extinction coefficient profiles. By comparison of the aerosol extinction coefficient retrieved by Mie-scattering aerosol lidar and CCD lidar at night, the reliability of new experimental approach is verified.

AUTOMATIC AND GENERIC MOSAICING OF MULTISENSOR IMAGES: AN APPLICATION TO PLEIADES HR

Directory of Open Access Journals (Sweden)

F. Bignalet-Cazalet

2012-07-01

Full Text Available In the early phase of the Pleiades program, the CNES (the French Space Agency specified and developed a fully automatic mosaicing processing unit, in order to generate satellite image mosaics under operational conditions. This tool can automatically put each input image in a common geometry, homogenize the radiometry, and generate orthomosaics using stitching lines. As the image quality commissioning phase of Pleiades1A is on-going, this mosaicing process is being tested for the first time under operational conditions. The French newly launched high resolution satellite can acquire adjacent images for French Civil and Defense User Ground Segments. This paper presents the very firsts results of mosaicing Pleiades1A images. Beyond Pleiades’ use, our mosaicing tool can process a significant variety of images, including other satellites and airborne acquisitions, using automatically-taken or external ground control points, offering time-based image superposition, and more. This paper also presents the design of the mosaicing tool and describes the processing workflow and the additional capabilities and applications.

Design principles and applications of a cooled CCD camera for electron microscopy.

Science.gov (United States)

Faruqi, A R

1998-01-01

Cooled CCD cameras offer a number of advantages in recording electron microscope images with CCDs rather than film which include: immediate availability of the image in a digital format suitable for further computer processing, high dynamic range, excellent linearity and a high detective quantum efficiency for recording electrons. In one important respect however, film has superior properties: the spatial resolution of CCD detectors tested so far (in terms of point spread function or modulation transfer function) are inferior to film and a great deal of our effort has been spent in designing detectors with improved spatial resolution. Various instrumental contributions to spatial resolution have been analysed and in this paper we discuss the contribution of the phosphor-fibre optics system in this measurement. We have evaluated the performance of a number of detector components and parameters, e.g. different phosphors (and a scintillator), optical coupling with lens or fibre optics with various demagnification factors, to improve the detector performance. The camera described in this paper, which is based on this analysis, uses a tapered fibre optics coupling between the phosphor and the CCD and is installed on a Philips CM12 electron microscope equipped to perform cryo-microscopy. The main use of the camera so far has been in recording electron diffraction patterns from two dimensional crystals of bacteriorhodopsin--from wild type and from different trapped states during the photocycle. As one example of the type of data obtained with the CCD camera a two dimensional Fourier projection map from the trapped O-state is also included. With faster computers, it will soon be possible to undertake this type of work on an on-line basis. Also, with improvements in detector size and resolution, CCD detectors, already ideal for diffraction, will be able to compete with film in the recording of high resolution images.

MULTIMODAL IMAGING OF MOSAIC RETINOPATHY IN CARRIERS OF HEREDITARY X-LINKED RECESSIVE DISEASES.

Science.gov (United States)

Wu, An-Lun; Wang, Jung-Pan; Tseng, Yun-Ju; Liu, Laura; Kang, Yu-Chuan; Chen, Kuan-Jen; Chao, An-Ning; Yeh, Lung-Kun; Chen, Tun-Lu; Hwang, Yih-Shiou; Wu, Wei-Chi; Lai, Chi-Chun; Wang, Nan-Kai

2018-05-01

To investigate the clinical features in carriers of X-linked retinitis pigmentosa, X-linked ocular albinism, and choroideremia (CHM) using multimodal imaging and to assess their diagnostic value in these three mosaic retinopathies. We prospectively examined 14 carriers of 3 X-linked recessive disorders (X-linked retinitis pigmentosa, X-linked ocular albinism, and CHM). Details of abnormalities of retinal morphology were evaluated using fundus photography, fundus autofluorescence (FAF) imaging, and spectral domain optical coherence tomography. In six X-linked retinitis pigmentosa carriers, fundus appearance varied from unremarkable to the presence of tapetal-like reflex and pigmentary changes. On FAF imaging, all carriers exhibited a bright radial reflex against a dark background. By spectral domain optical coherence tomography, loss of the ellipsoid zone in the macula was observed in 3 carriers (50%). Regarding the retinal laminar architecture, 4 carriers (66.7%) showed thinning of the outer nuclear layer and a dentate appearance of the outer plexiform layer. All five X-linked ocular albinism carriers showed a characteristic mud-splatter patterned fundus, dark radial streaks against a bright background on FAF imaging, and a normal-appearing retinal structure by spectral domain optical coherence tomography imaging. Two of the 3 CHM carriers (66.7%) showed a diffuse moth-eaten appearance of the fundus, and all 3 showed irregular hyper-FAF and hypo-FAF spots throughout the affected area. In the CHM carriers, the structural changes observed by spectral domain optical coherence tomography imaging were variable. Our findings in an Asian cohort suggest that FAF imaging is a practical diagnostic test for differentiating X-linked retinitis pigmentosa, X-linked ocular albinism, and CHM carriers. Wide-field FAF is an easy and helpful adjunct to testing for the correct diagnosis and identification of lyonization in carriers of these three mosaic retinopathies.

Systems, computer-implemented methods, and tangible computer-readable storage media for wide-field interferometry

Science.gov (United States)

Lyon, Richard G. (Inventor); Leisawitz, David T. (Inventor); Rinehart, Stephen A. (Inventor); Memarsadeghi, Nargess (Inventor)

2012-01-01

Disclosed herein are systems, computer-implemented methods, and tangible computer-readable storage media for wide field imaging interferometry. The method includes for each point in a two dimensional detector array over a field of view of an image: gathering a first interferogram from a first detector and a second interferogram from a second detector, modulating a path-length for a signal from an image associated with the first interferogram in the first detector, overlaying first data from the modulated first detector and second data from the second detector, and tracking the modulating at every point in a two dimensional detector array comprising the first detector and the second detector over a field of view for the image. The method then generates a wide-field data cube based on the overlaid first data and second data for each point. The method can generate an image from the wide-field data cube.

Upgrade of ESO's FIERA CCD Controller and PULPO Subsystem

Science.gov (United States)

Reyes-Moreno, J.; Geimer, C.; Balestra, A.; Haddad, N.

An overview of FIERA is presented with emphasis on its recent upgrade to PCI. The PCI board hosts two DSPs, one for real time control of the camera and another for on-the-fly processing of the incoming video data. In addition, the board is able to make DMA transfers, to synchronize to other boards alike, to be synchronized by a TIM bus and to control PULPO via RS232. The design is based on the IOP480 chip from PLX, for which we have developed a device driver for both Solaris and Linux. One computer is able to host more than one board and therefore can control an array of FIERA detector electronics. PULPO is a multifunctional subsystem widely used at ESO for the housekeeping of CCD cryostat heads and for shutter control. The upgrade of PULPO is based on an embedded PC running Linux. The upgraded PULPO is able to handle 29 temperature sensors, control 8 heaters and one shutter, read out one vacuum sensor and log any combination of parameters.

mosaicQA - A General Approach to Facilitate Basic Data Quality Assurance for Epidemiological Research.

Science.gov (United States)

Bialke, Martin; Rau, Henriette; Schwaneberg, Thea; Walk, Rene; Bahls, Thomas; Hoffmann, Wolfgang

2017-05-29

Epidemiological studies are based on a considerable amount of personal, medical and socio-economic data. To answer research questions with reliable results, epidemiological research projects face the challenge of providing high quality data. Consequently, gathered data has to be reviewed continuously during the data collection period. This article describes the development of the mosaicQA-library for non-statistical experts consisting of a set of reusable R functions to provide support for a basic data quality assurance for a wide range of application scenarios in epidemiological research. To generate valid quality reports for various scenarios and data sets, a general and flexible development approach was needed. As a first step, a set of quality-related questions, targeting quality aspects on a more general level, was identified. The next step included the design of specific R-scripts to produce proper reports for metric and categorical data. For more flexibility, the third development step focussed on the generalization of the developed R-scripts, e.g. extracting characteristics and parameters. As a last step the generic characteristics of the developed R functionalities and generated reports have been evaluated using different metric and categorical datasets. The developed mosaicQA-library generates basic data quality reports for multivariate input data. If needed, more detailed results for single-variable data, including definition of units, variables, descriptions, code lists and categories of qualified missings, can easily be produced. The mosaicQA-library enables researchers to generate reports for various kinds of metric and categorical data without the need for computational or scripting knowledge. At the moment, the library focusses on the data structure quality and supports the assessment of several quality indicators, including frequency, distribution and plausibility of research variables as well as the occurrence of missing and extreme values. To

Mosaic Down syndrome and acute lymphoblastic B cell-leukemia. Case report

Directory of Open Access Journals (Sweden)

Parra-Baltazar, Isabel Mónica

2016-10-01

Full Text Available Down syndrome (DS or trisomy 21 is a constitutional chromosomal abnormality, which may be mosaic in 1 % to 4 % of cases. DS mosaic diagnosis is difficult because most patients have a normal phenotype and show no significant clinical abnormalities. Patients with DS have a higher risk of developing acute leukemia such as acute lymphoblastic leukemia (ALL. We report the case of a 19-year old woman with mosaic trisomy 21 and ALL.

Apple Latent Spherical Virus Vector as Vaccine for the Prevention and Treatment of Mosaic Diseases in Pea, Broad Bean, and Eustoma Plants by Bean Yellow Mosaic Virus

Directory of Open Access Journals (Sweden)

Nozomi Satoh

2014-11-01

Full Text Available We investigated the protective effects of a viral vector based on an Apple latent spherical virus (ALSV harboring a segment of the Bean yellow mosaic virus (BYMV genome against mosaic diseases in pea, broad bean, and eustoma plants caused by BYMV infection. In pea plants pre-inoculated with the ALSV vaccine and challenge inoculated with BYMV expressing green fluorescence protein, BYMV multiplication occurred in inoculated leaves, but was markedly inhibited in the upper leaves. No mosaic symptoms due to BYMV infection were observed in the challenged plants pre-inoculated with the ALSV vaccine. Simultaneous inoculation with the ALSV vaccine and BYMV also prevented mosaic symptoms in broad bean and eustoma plants, and BYMV accumulation was strongly inhibited in the upper leaves of plants treated with the ALSV vaccine. Pea and eustoma plants were pre-inoculated with BYMV followed by inoculation with the ALSV vaccine to investigate the curative effects of the ALSV vaccine. In both plant species, recovery from mosaic symptoms was observed in upper leaves and BYMV accumulation was inhibited in leaves developing post-ALSV vaccination. These results show that ALSV vaccination not only prevents mosaic diseases in pea, broad bean, and eustoma, but that it is also effective in curing these diseases.

Evidence for a Complex Mosaic Genome Pattern in a Full-length Hepatitis C Virus Sequence

Directory of Open Access Journals (Sweden)

R.S. Ross

2008-01-01

Full Text Available The genome of the hepatitis C virus (HCV exhibits a high genetic variability. This remarkable heterogeneity is mainly attributed to the gradual accumulation of mutational changes, whereas the contribution of recombination events to the evolution of HCV remains controversial so far. While performing phylogenetic analyses including a large number of sequences deposited in the GenBank, we encountered a full-length HCV sequence (AY651061 that showed evidence for inter-subtype recombination and was, therefore, subjected to a detailed analysis of its molecular structure. The obtained results indicated that AY651061 does not represent a “simple” HCV 1c isolate, but a complex 1a/1c mosaic genome, showing five putative breakpoints in the core to NS3 regions. To our knowledge, this is the first report on a mosaic HCV full- length sequence with multiple breakpoints. The molecular structure of AY651061 is reminiscent of complex homologous recombinant variants occurring among other members of the flaviviridae family, e.g. GB virus C, dengue virus, and Japanese encephalitis virus. Our finding of a mosaic HCV sequence may have important implications for many fields of current HCV research which merit careful consideration.

An Interstitial 4q Deletion with a Mosaic Complementary Ring Chromosome in a Child with Dysmorphism, Linear Skin Pigmentation, and Hepatomegaly

Directory of Open Access Journals (Sweden)

J. Carter

2017-01-01

Full Text Available Interstitial deletions of 4q are rarely reported, vary in size, and have limited genotype-phenotype correlations. Here, genome-wide array CGH analysis identified a 21.6 Mb region of copy number loss at 4q12-q21.1 in a patient diagnosed with dysmorphism, linear skin pigmentation, and hepatomegaly. An additional small ring chromosome was detected in 5/30 cells examined via G-banding. Confirmation of the origin of the ring chromosome was obtained by FISH analysis which identified that the ring chromosome contained material from the deleted region of chromosome 4 and was therefore complementary to the 21.6 Mb deletion. Further microarray studies in the proband using a different microarray platform showed no evidence of mosaicism. This case highlights the importance of an integrated approach to cytogenetic analysis and demonstrates the value of G-banding for detecting mosaicism, as current microarray platforms are unable to detect low level mosaics.

Real-time tricolor phase measuring profilometry based on CCD sensitivity calibration

Science.gov (United States)

Zhu, Lin; Cao, Yiping; He, Dawu; Chen, Cheng

2017-02-01

A real-time tricolor phase measuring profilometry (RTPMP) based on charge coupled device (CCD) sensitivity calibration is proposed. Only one colour fringe pattern whose red (R), green (G) and blue (B) components are, respectively, coded as three sinusoidal phase-shifting gratings with an equivalent shifting phase of 2π/3 is needed and sent to an appointed flash memory on a specialized digital light projector (SDLP). A specialized time-division multiplexing timing sequence actively controls the SDLP to project the fringe patterns in R, G and B channels sequentially onto the measured object in one over seventy-two of a second and meanwhile actively controls a high frame rate monochrome CCD camera to capture the corresponding deformed patterns synchronously with the SDLP. So the sufficient information for reconstructing the three-dimensional (3D) shape in one over twenty-four of a second is obtained. Due to the different spectral sensitivity of the CCD camera to RGB lights, the captured deformed patterns from R, G and B channels cannot share the same peak and valley, which will lead to lower accuracy or even failing to reconstruct the 3D shape. So a deformed pattern amending method based on CCD sensitivity calibration is developed to guarantee the accurate 3D reconstruction. The experimental results verify the feasibility of the proposed RTPMP method. The proposed RTPMP method can obtain the 3D shape at over the video frame rate of 24 frames per second, avoid the colour crosstalk completely and be effective for measuring real-time changing object.

A List of Astronomical Meetings Available via Mosaic

Science.gov (United States)

Bryson, E.; Crabtree, D.

We have been making a list of astronomical and related meetings available electronically for a number of years. Recently, several meeting organizers have made information about their meetings available via anonymous ftp or even NCSA Mosaic. We have produced a new version of our electronic meeting list available via NCSA Mosaic which provides links to the information being provided electronically. Depending upon the amount of information being provided for an individual meeting, it may be possible for a user browsing the list of meetings to click on the meeting of interest, fill out a registration form, download maps, browse abstracts, etc. We hope the availability of this service will encourage other meeting organizers to make information about their meetings available electronically and to take advantage of new technology such as NCSA Mosaic. URL: http:// cadcwww.dao.nrc.calmeetings/meetings.html

Automated Generation of Geo-Referenced Mosaics From Video Data Collected by Deep-Submergence Vehicles: Preliminary Results

Science.gov (United States)

Rhzanov, Y.; Beaulieu, S.; Soule, S. A.; Shank, T.; Fornari, D.; Mayer, L. A.

2005-12-01

Many advances in understanding geologic, tectonic, biologic, and sedimentologic processes in the deep ocean are facilitated by direct observation of the seafloor. However, making such observations is both difficult and expensive. Optical systems (e.g., video, still camera, or direct observation) will always be constrained by the severe attenuation of light in the deep ocean, limiting the field of view to distances that are typically less than 10 meters. Acoustic systems can 'see' much larger areas, but at the cost of spatial resolution. Ultimately, scientists want to study and observe deep-sea processes in the same way we do land-based phenomena so that the spatial distribution and juxtaposition of processes and features can be resolved. We have begun development of algorithms that will, in near real-time, generate mosaics from video collected by deep-submergence vehicles. Mosaics consist of >>10 video frames and can cover 100's of square-meters. This work builds on a publicly available still and video mosaicking software package developed by Rzhanov and Mayer. Here we present the results of initial tests of data collection methodologies (e.g., transects across the seafloor and panoramas across features of interest), algorithm application, and GIS integration conducted during a recent cruise to the Eastern Galapagos Spreading Center (0 deg N, 86 deg W). We have developed a GIS database for the region that will act as a means to access and display mosaics within a geospatially-referenced framework. We have constructed numerous mosaics using both video and still imagery and assessed the quality of the mosaics (including registration errors) under different lighting conditions and with different navigation procedures. We have begun to develop algorithms for efficient and timely mosaicking of collected video as well as integration with navigation data for georeferencing the mosaics. Initial results indicate that operators must be properly versed in the control of the

Science case and requirements for the MOSAIC concept for a multi-object spectrograph for the European extremely large telescope

International Nuclear Information System (INIS)

Evans, C.J.; Puech, M.; Bonifacio, P.; Hammer, F.; Jagourel, P.; Caffau, E.; Disseau, K.; Flores, H.; Huertas-Company, M.; Mei, S.; Aussel, H.

2014-01-01

Over the past 18 months we have revisited the science requirements for a multi-object spectrograph (MOS) for the European Extremely Large Telescope (E-ELT). These efforts span the full range of E-ELT science and include input from a broad cross-section of astronomers across the ESO partner countries. In this contribution we summarise the key cases relating to studies of high-redshift galaxies, galaxy evolution, and stellar populations, with a more expansive presentation of a new case relating to detection of exoplanets in stellar clusters. A general requirement is the need for two observational modes to best exploit the large (=40 arcmin 2 ) patrol field of the E-ELT. The first mode ('high multiplex') requires integrated-light (or coarsely resolved) optical/near-IR spectroscopy of ≥100 objects simultaneously. The second ('high definition'), enabled by wide-field adaptive optics, requires spatially-resolved, near-IR of ≥10 objects/sub-fields. Within the context of the conceptual study for an ELT-MOS called MOSAIC, we summarise the top level requirements from each case and introduce the next steps in the design process. (authors)

Development of online cable eccentricity detection system based on X-ray CCD

International Nuclear Information System (INIS)

Chen Jianzhen; Li Bin; Wei Kaixia; Guo Lanying; Qu Guopu

2008-01-01

An improved technology of online cable eccentricity detection, based on X-ray CCD, greatly improves the measuring precision and the responding speed. The theory of eccentricity measuring based on X-ray CCD, and the structure of an apparatus are described. The apparatus is composed of scanning drive subsystem, X-ray generation components, data acquiring subsystem and high performance computer system. The measuring results are also presented. The features of this cable eccentricity detection technology are compared with the features of other technologies. (authors)

The Receiver System for the Ooty Wide Field Array

Indian Academy of Sciences (India)

The legacy Ooty Radio Telescope (ORT) is being reconfigured as a 264-element synthesis telescope, called the Ooty Wide Field Array (OWFA). Its antenna elements are the contiguous 1.92 m sections of the parabolic cylinder. It will operate in a 38-MHz frequency band centred at 326.5 MHz and will be equipped with a ...

Development of a Portable 3CCD Camera System for Multispectral Imaging of Biological Samples

Science.gov (United States)

Lee, Hoyoung; Park, Soo Hyun; Noh, Sang Ha; Lim, Jongguk; Kim, Moon S.

2014-01-01

Recent studies have suggested the need for imaging devices capable of multispectral imaging beyond the visible region, to allow for quality and safety evaluations of agricultural commodities. Conventional multispectral imaging devices lack flexibility in spectral waveband selectivity for such applications. In this paper, a recently developed portable 3CCD camera with significant improvements over existing imaging devices is presented. A beam-splitter prism assembly for 3CCD was designed to accommodate three interference filters that can be easily changed for application-specific multispectral waveband selection in the 400 to 1000 nm region. We also designed and integrated electronic components on printed circuit boards with firmware programming, enabling parallel processing, synchronization, and independent control of the three CCD sensors, to ensure the transfer of data without significant delay or data loss due to buffering. The system can stream 30 frames (3-waveband images in each frame) per second. The potential utility of the 3CCD camera system was demonstrated in the laboratory for detecting defect spots on apples. PMID:25350510

Steps toward a CONUS-wide reanalysis with archived NEXRAD data using National Mosaic and Multisensor Quantitative Precipitation Estimation (NMQ/Q2) algorithms

Science.gov (United States)

Stevens, S. E.; Nelson, B. R.; Langston, C.; Qi, Y.

2012-12-01

The National Mosaic and Multisensor QPE (NMQ/Q2) software suite, developed at NOAA's National Severe Storms Laboratory (NSSL) in Norman, OK, addresses a large deficiency in the resolution of currently archived precipitation datasets. Current standards, both radar- and satellite-based, provide for nationwide precipitation data with a spatial resolution of up to 4-5 km, with a temporal resolution as fine as one hour. Efforts are ongoing to process archived NEXRAD data for the period of record (1996 - present), producing a continuous dataset providing precipitation data at a spatial resolution of 1 km, on a timescale of only five minutes. In addition, radar-derived precipitation data are adjusted hourly using a wide variety of automated gauge networks spanning the United States. Applications for such a product range widely, from emergency management and flash flood guidance, to hydrological studies and drought monitoring. Results are presented from a subset of the NEXRAD dataset, providing basic statistics on the distribution of rainrates, relative frequency of precipitation types, and several other variables which demonstrate the variety of output provided by the software. Precipitation data from select case studies are also presented to highlight the increased resolution provided by this reanalysis and the possibilities that arise from the availability of data on such fine scales. A previously completed pilot project and steps toward a nationwide implementation are presented along with proposed strategies for managing and processing such a large dataset. Reprocessing efforts span several institutions in both North Carolina and Oklahoma, and data/software coordination are key in producing a homogeneous record of precipitation to be archived alongside NOAA's other Climate Data Records. Methods are presented for utilizing supercomputing capability in expediting processing, to allow for the iterative nature of a reanalysis effort.

The design and development of low- and high-voltage ASICs for space-borne CCD cameras

Science.gov (United States)

Waltham, N.; Morrissey, Q.; Clapp, M.; Bell, S.; Jones, L.; Torbet, M.

2017-12-01

The CCD remains the pre-eminent visible and UV wavelength image sensor in space science, Earth and planetary remote sensing. However, the design of space-qualified CCD readout electronics is a significant challenge with requirements for low-volume, low-mass, low-power, high-reliability and tolerance to space radiation. Space-qualified components are frequently unavailable and up-screened commercial components seldom meet project or international space agency requirements. In this paper, we describe an alternative approach of designing and space-qualifying a series of low- and high-voltage mixed-signal application-specific integrated circuits (ASICs), the ongoing development of two low-voltage ASICs with successful flight heritage, and two new high-voltage designs. A challenging sub-system of any CCD camera is the video processing and digitisation electronics. We describe recent developments to improve performance and tolerance to radiation-induced single event latchup of a CCD video processing ASIC originally developed for NASA's Solar Terrestrial Relations Observatory and Solar Dynamics Observatory. We also describe a programme to develop two high-voltage ASICs to address the challenges presented with generating a CCD's bias voltages and drive clocks. A 0.35 μm, 50 V tolerant, CMOS process has been used to combine standard low-voltage 3.3 V transistors with high-voltage 50 V diffused MOSFET transistors that enable output buffers to drive CCD bias drains, gates and clock electrodes directly. We describe a CCD bias voltage generator ASIC that provides 24 independent and programmable 0-32 V outputs. Each channel incorporates a 10-bit digital-to-analogue converter, provides current drive of up to 20 mA into loads of 10 μF, and includes current-limiting and short-circuit protection. An on-chip telemetry system with a 12-bit analogue-to-digital converter enables the outputs and multiple off-chip camera voltages to be monitored. The ASIC can drive one or more CCDs and

Micrometer and CCD measurements of double stars (Series 51

Directory of Open Access Journals (Sweden)

Popović G.M.

1998-01-01

Full Text Available 36 micrometric measurements of 20 double or multiple systems carried out with the Zeiss 65/1055 cm Refractor of Belgrade Observatory are communicated. Also 35 CCD measurements of 15 double or multiple systems are included.

Mosaic HIV envelope immunogenic polypeptides

Science.gov (United States)

Korber, Bette T. M.; Gnanakaran, S.; Perkins, Simon; Sodroski, Joseph; Haynes, Barton

2018-01-02

Disclosed herein are mosaic HIV envelope (Env) polypeptides that can elicit an immune response to HIV (such as cytotoxic T cell (CTL), helper T cell, and/or humoral responses). Also disclosed are sets of the disclosed mosaic Env polypeptides, which include two or more (for example, three) of the polypeptides. Also disclosed herein are methods for treating or inhibiting HIV in a subject including administering one or more of the disclosed immunogenic polypeptides or compositions to a subject infected with HIV or at risk of HIV infection. In some embodiments, the methods include inducing an immune response to HIV in a subject comprising administering to the subject at least one (such as two, three, or more) of the immunogenic polypeptides or at least one (such as two, three, or more) nucleic acids encoding at least one of the immunogenic polypeptides disclosed herein.

Wide-field optical coherence tomography based microangiography for retinal imaging

Science.gov (United States)

Zhang, Qinqin; Lee, Cecilia S.; Chao, Jennifer; Chen, Chieh-Li; Zhang, Thomas; Sharma, Utkarsh; Zhang, Anqi; Liu, Jin; Rezaei, Kasra; Pepple, Kathryn L.; Munsen, Richard; Kinyoun, James; Johnstone, Murray; van Gelder, Russell N.; Wang, Ruikang K.

2016-02-01

Optical coherence tomography angiography (OCTA) allows for the evaluation of functional retinal vascular networks without a need for contrast dyes. For sophisticated monitoring and diagnosis of retinal diseases, OCTA capable of providing wide-field and high definition images of retinal vasculature in a single image is desirable. We report OCTA with motion tracking through an auxiliary real-time line scan ophthalmoscope that is clinically feasible to image functional retinal vasculature in patients, with a coverage of more than 60 degrees of retina while still maintaining high definition and resolution. We demonstrate six illustrative cases with unprecedented details of vascular involvement in retinal diseases. In each case, OCTA yields images of the normal and diseased microvasculature at all levels of the retina, with higher resolution than observed with fluorescein angiography. Wide-field OCTA technology will be an important next step in augmenting the utility of OCT technology in clinical practice.

Two-dimensional spectrophotometry of planetary nebulae by CCD imaging

International Nuclear Information System (INIS)

Jacoby, G.H.; Africano, J.L.; Quigley, R.J.; Western Washington Univ., Bellingham, WA)

1987-01-01

The spatial distribution of the electron temperature and density and the ionic abundances of O(+), O(2+), N(+), and S(+) have been derived from CCD images of the planetary nebulae NGC 40 and NGC 6826 taken in the important emission lines of forbidden O II, forbidden O III, H-beta, forbidden N II, and forbidden S II. The steps required in the derivation of the absolute fluxes, line, ratios, and ionic abundances are outlined and then discussed in greater detail. The results show that the CCD imaging technique for two-dimensional spectrophotometry can effectively compete with classical spectrophotometry, providing the added benefits of complete spatial coverage at seeing-disk spatial resolution. The multiplexing in the spatial dimension, however, results in a loss of spectral information, since only one emission line is observed at any one time. 37 references

The Wide Field Imager of the International X-ray Observatory

Energy Technology Data Exchange (ETDEWEB)

Stefanescu, A., E-mail: astefan@hll.mpg.d [Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 Muenchen (Germany); Johannes Gutenberg-Universitaet, Inst. f. anorganische und analytische Chemie, 55099 Mainz (Germany); Bautz, M.W. [Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology, Cambridge, MA 02139-4307 (United States); Burrows, D.N. [Department of Astronomy and Astrophysics, Pennsylvania State University, University Park, PA 16802 (United States); Bombelli, L.; Fiorini, C. [Politecnico di Milano, Dipartimento di Elettronica e Informazione, Milano (Italy); INFN Sezione di Milano, Milano (Italy); Fraser, G. [Space Research Centre, Department of Physics and Astronomy, University of Leicester, University Road, Leicester LE1 7RH (United Kingdom); Heinzinger, K. [PNSensor GmbH, Roemerstr. 28, 80803 Muenchen (Germany); Herrmann, S. [Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 Muenchen (Germany); Max-Planck-Institut fuer extraterrestrische Physik, Giessenbachstr., 85748 Garching (Germany); Kuster, M. [Technische Universitaet Darmstadt, Institut fuer Kernphysik, Schlossgartenstr. 9, 64289 Darmstadt (Germany); Lauf, T. [Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 Muenchen (Germany); Max-Planck-Institut fuer extraterrestrische Physik, Giessenbachstr., 85748 Garching (Germany); Lechner, P. [PNSensor GmbH, Roemerstr. 28, 80803 Muenchen (Germany); Lutz, G. [Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 Muenchen (Germany); Max-Planck-Institut fuer Physik, Foehringer Ring 6, 80805 Muenchen (Germany); Majewski, P. [PNSensor GmbH, Roemerstr. 28, 80803 Muenchen (Germany); Meuris, A. [Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 Muenchen (Germany); Max-Planck-Institut fuer extraterrestrische Physik, Giessenbachstr., 85748 Garching (Germany); Murray, S.S. [Harvard/Smithsonian Center for Astrophysics, 60 Garden St., Cambridge, MA 02138 (United States)

2010-12-11

The International X-ray Observatory (IXO) will be a joint X-ray observatory mission by ESA, NASA and JAXA. It will have a large effective area (3 m{sup 2} at 1.25 keV) grazing incidence mirror system with good angular resolution (5 arcsec at 0.1-10 keV) and will feature a comprehensive suite of scientific instruments: an X-ray Microcalorimeter Spectrometer, a High Time Resolution Spectrometer, an X-ray Polarimeter, an X-ray Grating Spectrometer, a Hard X-ray Imager and a Wide-Field Imager. The Wide Field Imager (WFI) has a field-of-view of 18 ftx18 ft. It will be sensitive between 0.1 and 15 keV, offer the full angular resolution of the mirrors and good energy resolution. The WFI will be implemented as a 6 in. wafer-scale monolithical array of 1024x1024 pixels of 100x100{mu}m{sup 2} size. The DEpleted P-channel Field-Effect Transistors (DEPFET) forming the individual pixels are devices combining the functionalities of both detector and amplifier. Signal electrons are collected in a potential well below the transistor's gate, modulating the transistor current. Even when the device is powered off, the signal charge is collected and kept in the potential well below the gate until it is explicitly cleared. This makes flexible and fast readout modes possible.

Familial recurrences of FOXG1-related disorder: Evidence for mosaicism.

Science.gov (United States)

McMahon, Kelly Q; Papandreou, Apostolos; Ma, Mandy; Barry, Brenda J; Mirzaa, Ghayda M; Dobyns, William B; Scott, Richard H; Trump, Natalie; Kurian, Manju A; Paciorkowski, Alex R

2015-12-01

FOXG1-related disorders are caused by heterozygous mutations in FOXG1 and result in a spectrum of neurodevelopmental phenotypes including postnatal microcephaly, intellectual disability with absent speech, epilepsy, chorea, and corpus callosum abnormalities. The recurrence risk for de novo mutations in FOXG1-related disorders is assumed to be low. Here, we describe three unrelated sets of full siblings with mutations in FOXG1 (c.515_577del63, c.460dupG, and c.572T > G), representing familial recurrence of the disorder. In one family, we have documented maternal somatic mosaicism for the FOXG1 mutation, and all of the families presumably represent parental gonadal (or germline) mosaicism. To our knowledge, mosaicism has not been previously reported in FOXG1-related disorders. Therefore, this report provides evidence that germline mosaicism for FOXG1 mutations is a likely explanation for familial recurrence and should be considered during recurrence risk counseling for families of children with FOXG1-related disorders. © 2015 Wiley Periodicals, Inc.

Laser jamming experiment of varifocal colour CCD imaging system%变焦彩色CCD成像系统的激光干扰实验

Institute of Scientific and Technical Information of China (English)

汤伟; 王锐; 王挺峰; 郭劲

2017-01-01

Out-field laser jamming experiment of varifocal colour CCD imaging system irradiated by semiconductor laser was done.Laser jamming effects of colour CCD imaging system with different focal lengths were measured.Laser jamming model was set-up,and theoretical proving and analysis on experimental results were completed.Theorical and experimental results show that laser jamming effect of colour CCD imaging system irradiated by 750 nm laser is obvious,and CCD surface appears obvious light saturation and crosstalk phenomena.In the same initial laser irradiating conduction,laser power truncated by the aperture gradually decreases with increase of focal length f,and light saturation area on the CCD surface gradually increases.When focal length f of colour CCD imaging system is 17 mm,light saturation area on the CCD surface is 0.33 mm×0.29 mm.While focal length f of colour CCD imaging system increases to 120 mm,light saturation area on the CCD surface is 1.8 mm×1.2 mm.Simulation results are coincident with experimental results,and it proves laser jamming model is correct.The conclusions have a reference value for colour CCD in the practical application.%开展了变焦彩色CCD成像系统的激光外场干扰实验,测得了半导体激光(750 nm)对变焦距(17～187 mm)彩色CCD相机的干扰效果;同时利用典型的激光干扰CCD模型,完成了对实验结果的验证与理论分析.理论与实验结果表明:750 nm激光对彩色CCD成像系统的干扰效果明显,CCD靶面出现了明显的光饱和和串扰现象;在激光辐照条件相同情况下,光学系统焦距f越大,被光阑截断的激光就越少,到靶的激光功率密度就越高,CCD靶面的光饱和面积就越大;光学系统焦距f为17mm时,CCD靶面的光饱和面积为0.33 mm×0.29 mm,而当光学系统焦距f增大至120 mm时,CCD靶面的光饱和面积为1.8 mm×1.2 mm.仿真结果与实验结果基本一致,证明了理论模型的正确性.研究结果将对CCD器件的实际应用具有一定的指导意义.

A fast and automatic mosaic method for high-resolution satellite images

Science.gov (United States)

Chen, Hongshun; He, Hui; Xiao, Hongyu; Huang, Jing

2015-12-01

We proposed a fast and fully automatic mosaic method for high-resolution satellite images. First, the overlapped rectangle is computed according to geographical locations of the reference and mosaic images and feature points on both the reference and mosaic images are extracted by a scale-invariant feature transform (SIFT) algorithm only from the overlapped region. Then, the RANSAC method is used to match feature points of both images. Finally, the two images are fused into a seamlessly panoramic image by the simple linear weighted fusion method or other method. The proposed method is implemented in C++ language based on OpenCV and GDAL, and tested by Worldview-2 multispectral images with a spatial resolution of 2 meters. Results show that the proposed method can detect feature points efficiently and mosaic images automatically.

SlCCD7 controls strigolactone biosynthesis, shoot branching and mycorrhiza-induced apocarotenoid formation in tomato.

NARCIS (Netherlands)

Vogel, J.T.; Walter, M.H.; Giavalisco, P.; Lytovchenko, A.; Kohlen, W.; Charnikhova, T.; Simkin, A.J.; Goulet, C.; Strack, D.; Bouwmeester, H.J.; Fernie, A.R.; Klee, H.J.

2010-01-01

The regulation of shoot branching is an essential determinant of plant architecture, integrating multiple external and internal signals. One of the signaling pathways regulating branching involves the MAX (more axillary branches) genes. Two of the genes within this pathway, MAX3/CCD7 and MAX4/CCD8,

Reproductive history of a healthy woman with mosaic duplication of chromosome 4p.

Science.gov (United States)

Bernardini, Laura; Sinibaldi, Lorenzo; Ceccarini, Caterina; Novelli, Antonio; Dallapiccola, Bruno

2005-04-01

Mosaic autosomal duplications are rare and often result in mental retardation and congenital anomalies. Phenotype is not predictable depending on the chromosomal imbalance involved and the percentage and tissues distribution of unbalanced cells. We report on a young woman carrying a mosaic duplication of chromosome 4p, evaluated because of three abortions due to IUGR and fetal malformation. Mosaic dup(4p) was detected by standard and molecular cytogenetics. Unbalanced cells accounted for about 20 to 30% of nuclei in four examined tissues and did not cause any obvious phenotypic effect. It is likely that mosaic duplications are underascertained because they are not associated with obvious clinical effects in some individuals. Prenatal diagnosis is the method of choice to predict the karyotype in the offspring of subjects carrying mosaic chromosome imbalances.

New low noise CCD cameras for Pi-of-the-Sky project

Science.gov (United States)

Kasprowicz, G.; Czyrkowski, H.; Dabrowski, R.; Dominik, W.; Mankiewicz, L.; Pozniak, K.; Romaniuk, R.; Sitek, P.; Sokolowski, M.; Sulej, R.; Uzycki, J.; Wrochna, G.

2006-10-01

Modern research trends require observation of fainter and fainter astronomical objects on large areas of the sky. This implies usage of systems with high temporal and optical resolution with computer based data acquisition and processing. Therefore Charge Coupled Devices (CCD) became so popular. They offer quick picture conversion with much better quality than film based technologies. This work is theoretical and practical study of the CCD based picture acquisition system. The system was optimized for "Pi of The Sky" project. But it can be adapted to another professional astronomical researches. The work includes issue of picture conversion, signal acquisition, data transfer and mechanical construction of the device.

Turner syndrome and 45,X/47,XXX mosaicism.

Science.gov (United States)

Akbas, E; Mutluhan, H; Savasoglu, K; Soylemez, F; Ozturk, I; Yazici, G

2009-01-01

The occurrence of double aneuploidy is a relatively rare phenomenon. We report on a 17-year-old woman with short stature, minimal pubic and axillar hair and short hands. In cultured lymphocyte a double aneuploidy mosaicism was detected, consisting of a cell line with trisomy for X chromosome and a cell line with monosomy for the X-chromosome and no cell line with a normal karyotype. To our knowledge, this is the first case of mosaic 45,X/47,XXX in Turkey.

Numerical simulations and analyses of temperature control loop heat pipe for space CCD camera

Science.gov (United States)

Meng, Qingliang; Yang, Tao; Li, Chunlin

2016-10-01

As one of the key units of space CCD camera, the temperature range and stability of CCD components affect the image's indexes. Reasonable thermal design and robust thermal control devices are needed. One kind of temperature control loop heat pipe (TCLHP) is designed, which highly meets the thermal control requirements of CCD components. In order to study the dynamic behaviors of heat and mass transfer of TCLHP, particularly in the orbital flight case, a transient numerical model is developed by using the well-established empirical correlations for flow models within three dimensional thermal modeling. The temperature control principle and details of mathematical model are presented. The model is used to study operating state, flow and heat characteristics based upon the analyses of variations of temperature, pressure and quality under different operating modes and external heat flux variations. The results indicate that TCLHP can satisfy the thermal control requirements of CCD components well, and always ensure good temperature stability and uniformity. By comparison between flight data and simulated results, it is found that the model is to be accurate to within 1°C. The model can be better used for predicting and understanding the transient performance of TCLHP.

Short-channel field-effect transistors with 9-atom and 13-atom wide graphene nanoribbons.

Science.gov (United States)

Llinas, Juan Pablo; Fairbrother, Andrew; Borin Barin, Gabriela; Shi, Wu; Lee, Kyunghoon; Wu, Shuang; Yong Choi, Byung; Braganza, Rohit; Lear, Jordan; Kau, Nicholas; Choi, Wonwoo; Chen, Chen; Pedramrazi, Zahra; Dumslaff, Tim; Narita, Akimitsu; Feng, Xinliang; Müllen, Klaus; Fischer, Felix; Zettl, Alex; Ruffieux, Pascal; Yablonovitch, Eli; Crommie, Michael; Fasel, Roman; Bokor, Jeffrey

2017-09-21

Bottom-up synthesized graphene nanoribbons and graphene nanoribbon heterostructures have promising electronic properties for high-performance field-effect transistors and ultra-low power devices such as tunneling field-effect transistors. However, the short length and wide band gap of these graphene nanoribbons have prevented the fabrication of devices with the desired performance and switching behavior. Here, by fabricating short channel (L ch  ~ 20 nm) devices with a thin, high-κ gate dielectric and a 9-atom wide (0.95 nm) armchair graphene nanoribbon as the channel material, we demonstrate field-effect transistors with high on-current (I on  > 1 μA at V d  = -1 V) and high I on /I off  ~ 10 5 at room temperature. We find that the performance of these devices is limited by tunneling through the Schottky barrier at the contacts and we observe an increase in the transparency of the barrier by increasing the gate field near the contacts. Our results thus demonstrate successful fabrication of high-performance short-channel field-effect transistors with bottom-up synthesized armchair graphene nanoribbons.Graphene nanoribbons show promise for high-performance field-effect transistors, however they often suffer from short lengths and wide band gaps. Here, the authors use a bottom-up synthesis approach to fabricate 9- and 13-atom wide ribbons, enabling short-channel transistors with 10 5 on-off current ratio.

CCD photometry of apparent dwarf galaxies in Fornax

International Nuclear Information System (INIS)

Phillipps, S.; Grimley, P.L.; Disney, M.J.; Cawson, M.G.M.; Kibblewhite, E.J.

1986-01-01

Blue and red CCD surface photometry of two apparent dwarf galaxies in the Fornax cluster region is presented. Luminosity profiles are derived and their form discussed. The fainter galaxy resembles an archetypal diffuse dwarf elliptical but the brighter of the pair is either an unusual red dwarf or a background galaxy in chance juxtaposition. (author)

Meaning of visualizing retinal cone mosaic on adaptive optics images.

Science.gov (United States)

Jacob, Julie; Paques, Michel; Krivosic, Valérie; Dupas, Bénédicte; Couturier, Aude; Kulcsar, Caroline; Tadayoni, Ramin; Massin, Pascale; Gaudric, Alain

2015-01-01

To explore the anatomic correlation of the retinal cone mosaic on adaptive optics images. Retrospective nonconsecutive observational case series. A retrospective review of the multimodal imaging charts of 6 patients with focal alteration of the cone mosaic on adaptive optics was performed. Retinal diseases included acute posterior multifocal placoid pigment epitheliopathy (n = 1), hydroxychloroquine retinopathy (n = 1), and macular telangiectasia type 2 (n = 4). High-resolution retinal images were obtained using a flood-illumination adaptive optics camera. Images were recorded using standard imaging modalities: color and red-free fundus camera photography; infrared reflectance scanning laser ophthalmoscopy, fluorescein angiography, indocyanine green angiography, and spectral-domain optical coherence tomography (OCT) images. On OCT, in the marginal zone of the lesions, a disappearance of the interdigitation zone was observed, while the ellipsoid zone was preserved. Image recording demonstrated that such attenuation of the interdigitation zone co-localized with the disappearance of the cone mosaic on adaptive optics images. In 1 case, the restoration of the interdigitation zone paralleled that of the cone mosaic after a 2-month follow-up. Our results suggest that the interdigitation zone could contribute substantially to the reflectance of the cone photoreceptor mosaic. The absence of cones on adaptive optics images does not necessarily mean photoreceptor cell death. Copyright © 2015 Elsevier Inc. All rights reserved.

Somatic APC mosaicism and oligogenic inheritance in genetically unsolved colorectal adenomatous polyposis patients.

Science.gov (United States)

Ciavarella, Michele; Miccoli, Sara; Prossomariti, Anna; Pippucci, Tommaso; Bonora, Elena; Buscherini, Francesco; Palombo, Flavia; Zuntini, Roberta; Balbi, Tiziana; Ceccarelli, Claudio; Bazzoli, Franco; Ricciardiello, Luigi; Turchetti, Daniela; Piazzi, Giulia

2018-03-01

Germline variants in the APC gene cause familial adenomatous polyposis. Inherited variants in MutYH, POLE, POLD1, NTHL1, and MSH3 genes and somatic APC mosaicism have been reported as alternative causes of polyposis. However, ~30-50% of cases of polyposis remain genetically unsolved. Thus, the aim of this study was to investigate the genetic causes of unexplained adenomatous polyposis. Eight sporadic cases with >20 adenomatous polyps by 35 years of age or >50 adenomatous polyps by 55 years of age, and no causative germline variants in APC and/or MutYH, were enrolled from a cohort of 56 subjects with adenomatous colorectal polyposis. APC gene mosaicism was investigated on DNA from colonic adenomas by Sanger sequencing or Whole Exome Sequencing (WES). Mosaicism extension to other tissues (peripheral blood, saliva, hair follicles) was evaluated using Sanger sequencing and/or digital PCR. APC second hit was investigated in adenomas from mosaic patients. WES was performed on DNA from peripheral blood to identify additional polyposis candidate variants. We identified APC mosaicism in 50% of patients. In three cases mosaicism was restricted to the colon, while in one it also extended to the duodenum and saliva. One patient without APC mosaicism, carrying an APC in-frame deletion of uncertain significance, was found to harbor rare germline variants in OGG1, POLQ, and EXO1 genes. In conclusion, our restrictive selection criteria improved the detection of mosaic APC patients. In addition, we showed for the first time that an oligogenic inheritance of rare variants might have a cooperative role in sporadic colorectal polyposis onset.

Mosaic serine proteases in the mammalian central nervous system.

Science.gov (United States)

Mitsui, Shinichi; Watanabe, Yoshihisa; Yamaguchi, Tatsuyuki; Yamaguchi, Nozomi

2008-01-01

We review the structure and function of three kinds of mosaic serine proteases expressed in the mammalian central nervous system (CNS). Mosaic serine proteases have several domains in the proenzyme fragment, which modulate proteolytic function, and a protease domain at the C-terminus. Spinesin/TMPRSS5 is a transmembrane serine protease whose presynaptic distribution on motor neurons in the spinal cord suggests that it is significant for neuronal plasticity. Cell type-specific alternative splicing gives this protease diverse functions by modulating its intracellular localization. Motopsin/PRSS12 is a mosaic protease, and loss of its function causes mental retardation. Recent reports indicate the significance of this protease for cognitive function. We mention the fibrinolytic protease, tissue plasminogen activator (tPA), which has physiological and pathological functions in the CNS.

A comparison of image restoration approaches applied to three-dimensional confocal and wide-field fluorescence microscopy.

Science.gov (United States)

Verveer, P. J; Gemkow, M. J; Jovin, T. M

1999-01-01

We have compared different image restoration approaches for fluorescence microscopy. The most widely used algorithms were classified with a Bayesian theory according to the assumed noise model and the type of regularization imposed. We considered both Gaussian and Poisson models for the noise in combination with Tikhonov regularization, entropy regularization, Good's roughness and without regularization (maximum likelihood estimation). Simulations of fluorescence confocal imaging were used to examine the different noise models and regularization approaches using the mean squared error criterion. The assumption of a Gaussian noise model yielded only slightly higher errors than the Poisson model. Good's roughness was the best choice for the regularization. Furthermore, we compared simulated confocal and wide-field data. In general, restored confocal data are superior to restored wide-field data, but given sufficient higher signal level for the wide-field data the restoration result may rival confocal data in quality. Finally, a visual comparison of experimental confocal and wide-field data is presented.

The development of high-speed 100 fps CCD camera

International Nuclear Information System (INIS)

Hoffberg, M.; Laird, R.; Lenkzsus, F.; Liu, C.; Rodricks, B.

1997-01-01

This paper describes the development of a high-speed CCD digital camera system. The system has been designed to use CCDs from various manufacturers with minimal modifications. The first camera built on this design utilizes a Thomson 512 x 512 pixel CCD as its sensor, which is read out from two parallel outputs at a speed of 15 MHz/pixel/output. The data undergo correlated double sampling after which it is digitized into 12 bits. The throughput of the system translates into 60 MB/second, which is either stored directly in a PC or transferred to a custom-designed VXI module. The PC data acquisition version of the camera can collect sustained data in real time that is limited to the memory installed in the PC. The VXI version of the camera, also controlled by a PC, stores 512 MB of real-time data before it must be read out to the PC disk storage. The uncooled CCD can be used either with lenses for visible light imaging or with a phosphor screen for X-ray imaging. This camera has been tested with a phosphor screen coupled to a fiber-optic face plate for high-resolution, high-speed X-ray imaging. The camera is controlled through a custom event-driven user-friendly Windows package. The pixel clock speed can be changed from 1 to 15 MHz. The noise was measured to be 1.05 bits at a 13.3 MHz pixel clock. This paper will describe the electronics, software, and characterizations that have been performed using both visible and X-ray photons. (orig.)

Sensitive radioimmunosorbent assay for the detection of plant viruses. [Cauliflower mosaic virus, lettuce mosaic virus

Energy Technology Data Exchange (ETDEWEB)

Ghabrial, S A; Shepherd, R J [Kentucky Univ., Lexington (USA); California Univ., Davis (USA))

1980-06-01

A simple and highly sensitive radioimmunosorbent assay (RISA) for the detection of plant viruses is described. The RISA procedure is a microplate method based on the principle of 'double-antibody sandwich' and follows essentially the protocol of the enzyme-linked immunosorbent assay (ELISA) (Clark and Adams, 1977), with the exception that /sup 125/I-labelled ..gamma..-globulin is substituted for the ..gamma..-globulin enzyme conjugate; the bound /sup 125/I-..gamma..-globulin is dissociated by acidification from the double-antibody sandwich. The radioactivity is proportional to virus concentration, and cauliflower mosaic virus (CaMV) and lettuce mosaic virus (LMV) could be detected at concentrations as low as 5 and 2 ng/ml, respectively. Direct evidence of the adverse effects of conjugation with enzyme on the binding abilities of antibodies is presented. The RISA procedure should prove valuable with viruses for which the ELISA values are too low to be dependable.

An astrometric standard field in omega Cen

Science.gov (United States)

Wyse, Rosemary

2003-07-01

We propose to obtain a high-precision astrometric standard in a two-step procedure. First, we will create a ground-based astrometric standard field around omega Cen down to V=22 with a 3 mas accuracy in positions and better than 0.5 mas/yr in proper motions. This standard will be used to obtain precise absolute plate solutions for selected WFPC2 CCD frames and refine the self-calibrated mean distortion solution for the WFPC2 CCD chips. This will eliminate systematic errors inherent in the self-calibration techniques down to the rms=0.3 mas level, thus opening new opportunities to perform precision astrometry with WFPC2 alone or in combination with the other HST imaging instruments. We will also address the issue of the distortion's variation which has a paramount significance for space astrometry such as spearheaded by the HST or being under development {SIM, GAIA}. Second, all reduced WFPC2 CCD frames will be combined into the two field catalogs {astrometric flat fields} of positions in omega Cen of unprecedented precision {s.e.=0.1 mas} down to V=22 and will be available to the GO community and readily applicable to calibrating the ACS.

Collages of granulation pictures

Science.gov (United States)

Dunn, R. B.; November, L. J.

Two small-area selection schemes are applied to CCD observations of solar granulation. One procedure, referred to as mosaic, divides a 128 x 128 array into 64 subarrays of 16 x 16 pixels; the rms contrast of the fine structure is measured and compared in order to develop a mosaic of the subarrays. The second technique, collage, involves calculating rms values within a sliding Gaussian window and gating the pixel into the final image. Methods for assessing seeing quality, which involve the calculation of rms after high-pass filtering, are examined; a simple high-pass filter or an edge-locating function can be utilized for filtering. The rms map is then formed from the convolution of a Gaussian with either the high-pass or the Laplacian filters. The usefulness of the two procedures is demonstrated by applying the mosaic and collage processes to data recorded on July 17, 1983 with a CCD device on the NSO/Sac Peak Vacuum Tower Telescope.

New Multibeam Bathymetry Mosaic at NOAA/NCEI

Science.gov (United States)

Varner, J. D.; Cartwright, J.; Rosenberg, A. M.; Amante, C.; Sutherland, M.; Jencks, J. H.

2017-12-01

NOAA's National Centers for Environmental Information (NCEI) maintains an ever-growing archive of multibeam bathymetric data acquired from U.S. and international government and academic sources. The data are partitioned in the individual survey files in which they were originally received, and are stored in various formats not directly accessible by popular analysis and visualization tools. In order to improve the discoverability and accessibility of the data, NCEI created a new Multibeam Bathymetry Mosaic. Each survey was gridded at 3 arcsecond cell size and organized in an ArcGIS mosaic dataset, which was published as a set of standards-based web services usable in desktop GIS and web clients. In addition to providing a "seamless" grid of all surveys, a filter can be applied to isolate individual surveys. Both depth values in meters and shaded relief visualizations are available. The product represents the current state of the archive; no QA/QC was performed on the data before being incorporated, and the mosaic will be updated incrementally as new surveys are added to the archive. We expect the mosaic will address customer needs for visualization/extraction that existing tools (e.g. NCEI's AutoGrid) are unable to meet, and also assist data managers in identifying problem surveys, missing data, quality control issues, etc. This project complements existing efforts such as the Global Multi-Resolution Topography Data Synthesis (GMRT) at LDEO. Comprehensive visual displays of bathymetric data holdings are invaluable tools for seafloor mapping initiatives, such as Seabed 2030, that will aid in minimizing data collection redundancies and ensuring that valuable data are made available to the broadest community.

Fire mosaics and reptile conservation in a fire-prone region.

Science.gov (United States)

Nimmo, D G; Kelly, L T; Spence-Bailey, L M; Watson, S J; Taylor, R S; Clarke, M F; Bennett, A F

2013-04-01

Fire influences the distribution of fauna in terrestrial biomes throughout the world. Use of fire to achieve a mosaic of vegetation in different stages of succession after burning (i.e., patch-mosaic burning) is a dominant conservation practice in many regions. Despite this, knowledge of how the spatial attributes of vegetation mosaics created by fire affect fauna is extremely scarce, and it is unclear what kind of mosaic land managers should aim to achieve. We selected 28 landscapes (each 12.6 km(2) ) that varied in the spatial extent and diversity of vegetation succession after fire in a 104,000 km(2) area in the semiarid region of southeastern Australia. We surveyed for reptiles at 280 sites nested within the 28 landscapes. The landscape-level occurrence of 9 of the 22 species modeled was associated with the spatial extent of vegetation age classes created by fire. Biogeographic context and the extent of a vegetation type influenced 7 and 4 species, respectively. No species were associated with the diversity of vegetation ages within a landscape. Negative relations between reptile occurrence and both extent of recently burned vegetation (≤10 years postfire, n = 6) and long unburned vegetation (>35 years postfire, n = 4) suggested that a coarse-grained mosaic of areas (e.g. >1000 ha) of midsuccessional vegetation (11-35 years postfire) may support the fire-sensitive reptile species we modeled. This age class coincides with a peak in spinifex cover, a keystone structure for reptiles in semiarid and arid Australia. Maintaining over the long term a coarse-grained mosaic of large areas of midsuccessional vegetation in mallee ecosystems will need to be balanced against the short-term negative effects of large fires on many reptile species and a documented preference by species from other taxonomic groups, particularly birds, for older vegetation. © 2012 Society for Conservation Biology.

Extended image differencing for change detection in UAV video mosaics

Science.gov (United States)

Saur, Günter; Krüger, Wolfgang; Schumann, Arne

2014-03-01

Change detection is one of the most important tasks when using unmanned aerial vehicles (UAV) for video reconnaissance and surveillance. We address changes of short time scale, i.e. the observations are taken in time distances from several minutes up to a few hours. Each observation is a short video sequence acquired by the UAV in near-nadir view and the relevant changes are, e.g., recently parked or moved vehicles. In this paper we extend our previous approach of image differencing for single video frames to video mosaics. A precise image-to-image registration combined with a robust matching approach is needed to stitch the video frames to a mosaic. Additionally, this matching algorithm is applied to mosaic pairs in order to align them to a common geometry. The resulting registered video mosaic pairs are the input of the change detection procedure based on extended image differencing. A change mask is generated by an adaptive threshold applied to a linear combination of difference images of intensity and gradient magnitude. The change detection algorithm has to distinguish between relevant and non-relevant changes. Examples for non-relevant changes are stereo disparity at 3D structures of the scene, changed size of shadows, and compression or transmission artifacts. The special effects of video mosaicking such as geometric distortions and artifacts at moving objects have to be considered, too. In our experiments we analyze the influence of these effects on the change detection results by considering several scenes. The results show that for video mosaics this task is more difficult than for single video frames. Therefore, we extended the image registration by estimating an elastic transformation using a thin plate spline approach. The results for mosaics are comparable to that of single video frames and are useful for interactive image exploitation due to a larger scene coverage.

KARAKTERISASICYMBIDIUM MOSAIC VIRUS (CYMMV PADA TANAMAN ANGGREK

Directory of Open Access Journals (Sweden)

KHAMDAN KHALIMI

2012-11-01

Full Text Available Characterization ofCymbidium mosaic virus (CymMV on Orchid Plant Orchids are affected by more virus disease problems than most crops, reducing their commercial values considerably. Orchid viruses are widespread in cultivated orchids, withCymbidium mosaic potexvirus (CymMV being the most prevalent. CymMV high incidence in cultivated orchids has been attributed to the stability and ease of transmission of this virus through cultural practices. CymMV induces floral and foliar necrosis. The virus also reduce plant vigor and lower flower quality, which affect their economic value. The objective of the research is to characterize the virus causing mosaic or chlorotic and necrotic on orchids in West Java. A reverse transcription-polymerase chain reaction (RT- PCR assays using oligonucleotide primers specific to CymMV were also successfully amplified the regions of the coat protein (CP gene of the virus. Analysis by using sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE revealed that the virus have a major structural protein with an estimated molecular weight of 28 kDa. Aligments of partial nucleotide sequences of the CP gene displayed 86 to 92% homology to CymMV isolates from other countries.

MOSAIC roadmap for mobile collaborative work related to health and wellbeing.

NARCIS (Netherlands)

Saranummi, Niilo; Jones, Valerie M.; Pallot, M.; Pawar, K.S.

2005-01-01

The objective of the MOSAIC project is to accelerate innovation in Mobile Worker Support Environments. For that purpose MOSAIC develops visions and illustrative scenarios for future collaborative workspaces involving mobile and location-aware working. Analysis of the scenarios is input to the

VizieR Online Data Catalog: Optical & Spitzer photometry in IC 1805 (Sung+, 2017)

Science.gov (United States)

Sung, H.; Bessell, M. S.; Chun, M.-Y.; Yi, J.; Naze, Y.; Lim, B.; Karimov, R.; Rauw, G.; Park, B.-G.; Hur, H.

2017-06-01

For a study of the IMF and the star-formation history of the young open cluster IC 1805, we obtained deep wide-field VRI and Hα images of IC 1805 using the CFH12K mosaic CCD camera of the CFHT on 2002 January 6 and 7. We also observed several regions in IC 1805, for a study of the reddening and massive star content, using the SITe 2000x800 CCD (Maidanak 2k CCD) and standard UBVRI filters of the AZT-22 1.5m telescope at the Maidanak Astronomical Observatory in Uzbekistan on 2003 August 18 and 2004 december 25,30. Later, we obtained additional images of the central region of IC 1805 with the Fairchild 486 CCD (SNUCam) and UBVI and Hα filters of the AZT-22 telescope on 2007 October 7 and 2009 January 19. The Spitzer mapping observations were performed on 2006 September 20 under program ID 20052 (PI: S. Wolff). For complete photometry of stars in the CFH12K FOV in 3.6 and 4.5um, we also downloaded and reduced the GLIMPSE360 data (AOR: 38753280, 38763264, 38769408, 38799104, 38798592, 38784512, PI: B. A. Whitney). MIPS scans of IC 1805 were obtained on 2005 August 31 and 2005 September 2 (PID 3234, PI: J. S. Greeves). The Chandra X-ray Observatory Observations of IC 1805 (ObsID: 7033, PI: L. Townley) were made on 2006 November 25. The total exposure time was about 79ks. The properties of 647 X-ray sources were published in Townsley+ (2014,J/ApJS/213/1). We searched for the optical and MIR counterparts of these X-ray sources with a matching radius of up to 1.5". (4 data files).

Multi-image mosaic with SIFT and vision measurement for microscale structures processed by femtosecond laser

Science.gov (United States)

Wang, Fu-Bin; Tu, Paul; Wu, Chen; Chen, Lei; Feng, Ding

2018-01-01

In femtosecond laser processing, the field of view of each image frame of the microscale structure is extremely small. In order to obtain the morphology of the whole microstructure, a multi-image mosaic with partially overlapped regions is required. In the present work, the SIFT algorithm for mosaic images was analyzed theoretically, and by using multiple images of a microgroove structure processed by femtosecond laser, a stitched image of the whole groove structure could be studied experimentally and realized. The object of our research concerned a silicon wafer with a microgroove structure ablated by femtosecond laser. First, we obtained microgrooves at a width of 380 μm at different depths. Second, based on the gray image of the microgroove, a multi-image mosaic with slot width and slot depth was realized. In order to improve the image contrast between the target and the background, and taking the slot depth image as an example, a multi-image mosaic was then realized using pseudo color enhancement. Third, in order to measure the structural size of the microgroove with the image, a known width streak ablated by femtosecond laser at 20 mW was used as a calibration sample. Through edge detection, corner extraction, and image correction for the streak images, we calculated the pixel width of the streak image and found the measurement ratio constant Kw in the width direction, and then obtained the proportional relationship between a pixel and a micrometer. Finally, circular spot marks ablated by femtosecond laser at 2 mW and 15 mW were used as test images, and proving that the value Kw was correct, the measurement ratio constant Kh in the height direction was obtained, and the image measurements for a microgroove of 380 × 117 μm was realized based on a measurement ratio constant Kw and Kh. The research and experimental results show that the image mosaic, image calibration, and geometric image parameter measurements for the microstructural image ablated by

Image Mosaic Method Based on SIFT Features of Line Segment

Directory of Open Access Journals (Sweden)

Jun Zhu

2014-01-01

Full Text Available This paper proposes a novel image mosaic method based on SIFT (Scale Invariant Feature Transform feature of line segment, aiming to resolve incident scaling, rotation, changes in lighting condition, and so on between two images in the panoramic image mosaic process. This method firstly uses Harris corner detection operator to detect key points. Secondly, it constructs directed line segments, describes them with SIFT feature, and matches those directed segments to acquire rough point matching. Finally, Ransac method is used to eliminate wrong pairs in order to accomplish image mosaic. The results from experiment based on four pairs of images show that our method has strong robustness for resolution, lighting, rotation, and scaling.

Influence of Fire Mosaics, Habitat Characteristics and Cattle Disturbance on Mammals in Fire-Prone Savanna Landscapes of the Northern Kimberley.

Directory of Open Access Journals (Sweden)

Ian J Radford

Full Text Available Patch mosaic burning, in which fire is used to produce a mosaic of habitat patches representative of a range of fire histories ('pyrodiversity', has been widely advocated to promote greater biodiversity. However, the details of desired fire mosaics for prescribed burning programs are often unspecified. Threatened small to medium-sized mammals (35 g to 5.5 kg in the fire-prone tropical savannas of Australia appear to be particularly fire-sensitive. Consequently, a clear understanding of which properties of fire mosaics are most instrumental in influencing savanna mammal populations is critical. Here we use mammal capture data, remotely sensed fire information (i.e. time since last fire, fire frequency, frequency of late dry season fires, diversity of post-fire ages in 3 km radius, and spatial extent of recently burnt, intermediate and long unburnt habitat and structural habitat attributes (including an index of cattle disturbance to examine which characteristics of fire mosaics most influence mammals in the north-west Kimberley. We used general linear models to examine the relationship between fire mosaic and habitat attributes on total mammal abundance and richness, and the abundance of the most commonly detected species. Strong negative associations of mammal abundance and richness with frequency of late dry season fires, the spatial extent of recently burnt habitat (post-fire age <1 year within 3 km radius and level of cattle disturbance were observed. Shrub cover was positively related to both mammal abundance and richness, and availability of rock crevices, ground vegetation cover and spatial extent of ≥4 years unburnt habitat were all positively associated with at least some of the mammal species modelled. We found little support for diversity of post-fire age classes in the models. Our results indicate that both a high frequency of intense late dry season fires and extensive, recently burnt vegetation are likely to be detrimental to

NMR of bicelles: orientation and mosaic spread of the liquid-crystal director under sample rotation

International Nuclear Information System (INIS)

Zandomeneghi, Giorgia; Tomaselli, Marco; Williamson, Philip T.F.; Meier, Beat H.

2003-01-01

Model-membrane systems composed of liquid-crystalline bicellar phases can be uniaxially oriented with respect to a magnetic field, thereby facilitating structural and dynamics studies of membrane-associated proteins. Here we quantitatively characterize a method that allows the manipulation of the direction of this uniaxial orientation. Bicelles formed from DMPC/DHPC are examined by 31 P NMR under variable-angle sample-spinning (VAS) conditions, confirming that the orientation of the liquid-crystalline director can be influenced by sample spinning. The director is perpendicular to the rotation axis when Θ (the angle between the sample-spinning axis and the magnetic field direction) is smaller than the magic angle, and is parallel to the rotation axis when Θ is larger than the magic angle. The new 31 P NMR VAS data presented are considerably more sensitive to the orientation of the bicelle than earlier 2 H studies and the analysis of the sideband pattern allows the determination of the orientation of the liquid-crystal director and its variation over the sample, i.e., the mosaic spread. Under VAS, the mosaic spread is small if Θ deviates significantly from the magic angle but becomes very large at the magic angle

Playing with the internet through world wide web

International Nuclear Information System (INIS)

Kim, Seon Tae; Jang, Jin Seok

1995-07-01

This book describes how to use the internet with world wide web. It is divided into six chapters, which are Let's go to the internet ocean, the internet in information superhighway are, connecting the world with a telephone wire such as link with the internet cable and telephone modem, internet service providers, text mode connection, Domain and IP address, the principle and use of world wide web ; business, music, fashion, movie and photo, internet news and e-mail, making internet map with web language, and from installation to application of base program such as TCP/IP, SLIP/PPP 3270 Emulator, Finger and NCSA Mosaic.

Promoting Cas9 degradation reduces mosaic mutations in non-human primate embryos

Science.gov (United States)

Tu, Zhuchi; Yang, Weili; Yan, Sen; Yin, An; Gao, Jinquan; Liu, Xudong; Zheng, Yinghui; Zheng, Jiezhao; Li, Zhujun; Yang, Su; Li, Shihua; Guo, Xiangyu; Li, Xiao-Jiang

2017-01-01

CRISPR-Cas9 is a powerful new tool for genome editing, but this technique creates mosaic mutations that affect the efficiency and precision of its ability to edit the genome. Reducing mosaic mutations is particularly important for gene therapy and precision genome editing. Although the mechanisms underlying the CRSIPR/Cas9-mediated mosaic mutations remain elusive, the prolonged expression and activity of Cas9 in embryos could contribute to mosaicism in DNA mutations. Here we report that tagging Cas9 with ubiquitin-proteasomal degradation signals can facilitate the degradation of Cas9 in non-human primate embryos. Using embryo-splitting approach, we found that shortening the half-life of Cas9 in fertilized zygotes reduces mosaic mutations and increases its ability to modify genomes in non-human primate embryos. Also, injection of modified Cas9 in one-cell embryos leads to live monkeys with the targeted gene modifications. Our findings suggest that modifying Cas9 activity can be an effective strategy to enhance precision genome editing. PMID:28155910

A FORTRAN version implementation of block adjustment of CCD frames and its preliminary application

Science.gov (United States)

Yu, Y.; Tang, Z.-H.; Li, J.-L.; Zhao, M.

2005-09-01

A FORTRAN version implementation of the block adjustment (BA) of overlapping CCD frames is developed and its flowchart is shown. The program is preliminarily applied to obtain the optical positions of four extragalactic radio sources. The results show that because of the increase in the number and sky coverage of reference stars the precision of optical positions with BA is improved compared with the single CCD frame adjustment.

BVR Standardization of the CCD Photometric System of Chungbuk National University Observatory

Directory of Open Access Journals (Sweden)

Jang-Hae Jeong

2009-06-01

Full Text Available BVR observations for 52 standard stars were performed using the 1-m reflecter with 2K CCD System of Chungbuk National University Observatory(CBNUO in 2008. We obtained 1,322 CCD images to establish a correlation between our bvr system and the standard Johnson-Cousins BVR system. We derived the tentative equations of transformation between then as follows; V = v-0.0303(B-V+0.0466 B-V = 1.3475(b-v-0.0251 V-R = 1.0641(v-r-0.0125 Using these equations the magnitudes in V, B-V, and V-R for 197 stars were obtained.

CCD Camera Lens Interface for Real-Time Theodolite Alignment

Science.gov (United States)

Wake, Shane; Scott, V. Stanley, III

2012-01-01

Theodolites are a common instrument in the testing, alignment, and building of various systems ranging from a single optical component to an entire instrument. They provide a precise way to measure horizontal and vertical angles. They can be used to align multiple objects in a desired way at specific angles. They can also be used to reference a specific location or orientation of an object that has moved. Some systems may require a small margin of error in position of components. A theodolite can assist with accurately measuring and/or minimizing that error. The technology is an adapter for a CCD camera with lens to attach to a Leica Wild T3000 Theodolite eyepiece that enables viewing on a connected monitor, and thus can be utilized with multiple theodolites simultaneously. This technology removes a substantial part of human error by relying on the CCD camera and monitors. It also allows image recording of the alignment, and therefore provides a quantitative means to measure such error.

Mosaic Turner syndrome and hyperinsulinaemic hypoglycaemia

DEFF Research Database (Denmark)

Alkhayyat, H.; Christesen, Henrik Thybo; Steer, J.

2006-01-01

BACKGROUND: A common and well recognised feature of Turner's syndrome (partial or total monosomy X) is impaired glucose tolerance or type 2 diabetes mellitus. A small percentage of patients with Turner's syndrome have a complex mosaic karyotype with atypical clinical features and mental retardation....... METHODS/PATIENT: We report the first case of a child with a complex mosaic Turner genotype and hyperinsulinaemic hypoglycaemia responsive to diazoxide therapy. RESULTS: Cytogenetic analysis showed four cell lines: one with 45,X; the others with an additional small ring chromosome, a small marker...... chromosome, and both the ring and marker chromosomes, respectively. FISH studies showed the abnormal chromosomes to originate from an X. The X inactivation locus (XIST) was present in the ring, but not in the marker chromosome. CONCLUSIONS: The recognition of hypoglycaemia in children with atypical Turner...

Mosaic Turner syndrome associated with schizophrenia.

Science.gov (United States)

Jung, Sook Young; Park, Joo Won; Kim, Dong Hyun; Jun, Yong Hoon; Lee, Jeong Seop; Lee, Ji Eun

2014-03-01

Turner syndrome is a sex-chromosome disorder; occurring in 1 in 2,500 female births. There are sporadic few case reports of concomitant Turner syndrome with schizophrenia worldwide. Most Turner females had a 45,X monosomy, whereas the majority of comorbidity between Turner syndrome and schizophrenia had a mosaic karyotype (45,X/46,XX). We present a case of a 21-year-old woman with Turner syndrome, mosaic karyotype (45,X/46,XX), showing mental retardation, hypothyroidism, and schizophrenia. HOPA gene within Xq13 is related to mental retardation, hypothyroidism, and schizophrenia. Our case may be a potential clue which supports the hypothesis for involvement of genes on X chromosome in development of schizophrenia. Further studies including comorbid cases reports are need in order to discern the cause of schizophrenia in patients having Turner syndrome.

Towards Luminescence Dating Of Mosaic Glass

Science.gov (United States)

Galli, A.; Martini, M.; Sibila, E.; Villa, I.

The possibility of dating archaeological glass by means of luminescent techniques has been investigated in recent years, despite the difficulties of this application, mainly linked to the amorphous structure of the material. We focused in particular on mosaic glass, after the encouraging results obtained on byzantine and medieval samples. Further studies were devoted to the comprehension of the luminescent mechanisms in silica glasses, and to the investigation of the relationships between luminescence, colouring or opacifier ions and crystalline phase of the vitreous matrix. The results of a study on the dosimetric characteristics of thermoluminescence (TL) and Optically Stimulated Luminescence (OSL) of a few medieval blue-green mosaic glasses from the San Lorenzo church (Milan) are presented, and the experimental protocols established to identify their suitability for dating are discussed.

Radiotherapy film densitometry using a slow-scan, cooled, digital CCD imaging system

International Nuclear Information System (INIS)

Burch, S.E.

1993-01-01

A method of performing high-resolution two-dimensional film densitometry for full size radiographic film (35 x 43 cm) using a cooled CCD camera was proposed. Studies were performed to evaluate the physical characteristics of the camera system and recommendations were made to assure maximum accuracy of density measurement. Test films of various sizes and densities, as well as clinical dosimetry films, were measured with the CCD densitometer and the reference transmission densitometer. The measured densities agreed within the stated accuracy of the transmission densitometer for all films with maximum density less than or equal to 1.5 optical density units. The 0.2 mm spatial resolution with 4096 shades of gray made it possible to study dose distributions even for films containing areas of high dose gradients. Patient verification radiographs were used to study exit beam dose distributions to detect errors in beam placement, patient position, and proper placement of beam modifying devices such as wedges and compensators. For studying photon beam dose distributions within phantoms, a method was developed using lead foils placed lateral to the film plane to filter very low energy scattered photons. The error in measurement of central axis percentage depth dose from film for 4 MV x-rays, 25 x 25 cm field was decreased from 65% to 4%. The method requires only two calibration films for density to dose conversion and represents an important advance in the field of film densitometry for radiotherapy. The technique was applied to the study of dynamic wedge dose distribution from a 6 MV linear accelerator. The phantom modification decreased the error in percentage depth dose from 21% to 1% for the 15 x 15 cm beam with 60 degree wedge angle. Profile off-axis errors for the same beam were decreased from 8% to 3%. The film dosimetry system provides fast, high resolution film density data for use in radiotherapy imaging and quality assurance

Association of VPg and eIF4E in the host tropism at the cellular level of Barley yellow mosaic virus and Wheat yellow mosaic virus in the genus Bymovirus.

Science.gov (United States)

Li, Huangai; Shirako, Yukio

2015-02-01

Barley yellow mosaic virus (BaYMV) and Wheat yellow mosaic virus (WYMV) are separate species in the genus Bymovirus with bipartite plus-sense RNA genomes. In fields, BaYMV infects only barley and WYMV infects only wheat. Here, we studied the replicative capability of the two viruses in barley and wheat mesophyll protoplasts. BaYMV replicated in both barley and wheat protoplasts, but WYMV replicated only in wheat protoplasts. The expression of wheat translation initiation factor 4E (eIF4E), a common host factor for potyviruses, from the WYMV genome enabled WYMV replication in barley protoplasts. Replacing the BaYMV VPg gene with that of WYMV abolished BaYMV replication in barley protoplasts, whereas the additional expression of wheat eIF4E from BaYMV genome restored the replication of the BaYMV mutant in barley protoplasts. These results indicate that both VPg and the host eIF4E are involved in the host tropism of BaYMV and WYMV at the replication level. Copyright © 2014 Elsevier Inc. All rights reserved.

A directional fast neutron detector using scintillating fibers and an intensified CCD camera system

International Nuclear Information System (INIS)

Holslin, Daniel; Armstrong, A.W.; Hagan, William; Shreve, David; Smith, Scott

1994-01-01

We have been developing and testing a scintillating fiber detector (SFD) for use as a fast neutron sensor which can discriminate against neutrons entering at angles non-parallel to the fiber axis (''directionality''). The detector/convertor component is a fiber bundle constructed of plastic scintillating fibers each measuring 10 cm long and either 0.3 mm or 0.5 mm in diameter. Extensive Monte Carlo simulations were made to optimize the bundle response to a range of fast neutron energies and to intense fluxes of high energy gamma-rays. The bundle is coupled to a set of gamma-ray insenitive electro-optic intensifiers whose output is viewed by a CCD camera directly coupled to the intensifiers. Two types of CCD cameras were utilized: 1) a standard, interline RS-170 camera with electronic shuttering and 2) a high-speed (up to 850 frame/s) field-transfer camera. Measurements of the neutron detection efficiency and directionality were made using 14 MeV neutrons, and the response to gamma-rays was performed using intense fluxes from radioisotopic sources (up to 20 R/h). Recently, the detector was constructed and tested using a large 10 cm by 10 cm square fiber bundle coupled to a 10 cm diameter GEN I intensifier tube. We present a description of the various detector systems and report the results of experimental tests. ((orig.))

Deployment of the Hobby-Eberly Telescope wide-field upgrade

Science.gov (United States)

Hill, Gary J.; Drory, Niv; Good, John M.; Lee, Hanshin; Vattiat, Brian L.; Kriel, Herman; Ramsey, Jason; Bryant, Randy; Elliot, Linda; Fowler, Jim; Häuser, Marco; Landiau, Martin; Leck, Ron; Odewahn, Stephen; Perry, Dave; Savage, Richard; Schroeder Mrozinski, Emily; Shetrone, Matthew; DePoy, D. L.; Prochaska, Travis; Marshall, J. L.; Damm, George; Gebhardt, Karl; MacQueen, Phillip J.; Martin, Jerry; Armandroff, Taft; Ramsey, Lawrence W.

2016-07-01

The Hobby-Eberly Telescope (HET) is an innovative large telescope, located in West Texas at the McDonald Observatory. The HET operates with a fixed segmented primary and has a tracker, which moves the four-mirror corrector and prime focus instrument package to track the sidereal and non-sidereal motions of objects. We have completed a major multi-year upgrade of the HET that has substantially increased the pupil size to 10 meters and the field of view to 22 arcminutes by replacing the corrector, tracker, and prime focus instrument package. The new wide field HET will feed the revolutionary integral field spectrograph called VIRUS, in support of the Hobby-Eberly Telescope Dark Energy Experiment (HETDEX§), a new low resolution spectrograph (LRS2), an upgraded high resolution spectrograph (HRS2), and later the Habitable Zone Planet Finder (HPF). The upgrade is being commissioned and this paper discusses the completion of the installation, the commissioning process and the performance of the new HET.

Enabling Large Focal Plane Arrays Through Mosaic Hybridization

Science.gov (United States)

Miller, Timothy M.; Jhabvala, Christine A.; Leong, Edward; Costen, Nicholas P.; Sharp, Elmer; Adachi, Tomoko; Benford, Dominic J.

2012-01-01

We have demonstrated advances in mosaic hybridization that will enable very large format far-infrared detectors. Specifically we have produced electrical detector models via mosaic hybridization yielding superconducting circuit paths by hybridizing separately fabricated sub-units onto a single detector unit. The detector model was made on a 100mm diameter wafer while four model readout quadrant chips were made from a separate 100mm wafer. The individually fabricated parts were hybridized using a flip-chip bonder to assemble the detector-readout stack. Once all of the hybridized readouts were in place, a single, large and thick silicon substrate was placed on the stack and attached with permanent epoxy to provide strength and a Coefficient of Thermal Expansion match to the silicon components underneath. Wirebond pads on the readout chips connect circuits to warm readout electronics; and were used to validate the successful superconducting electrical interconnection of the model mosaic-hybrid detector. This demonstration is directly scalable to 150 mm diameter wafers, enabling pixel areas over ten times the area currently available.

LANDSAT M. S. S. IMAGE MOSAIC OF TUNISIA.

Science.gov (United States)

Boswell-Thomas, J. C.; ,

1984-01-01

The Landsat mosaic of Tunisia funded by USAID for the Remote Sensing Laboratory, Soils Division, Ministry of Agriculture, Tunisia, was completed by the USGS in September 1983. It is a mixed mosaic associating digital corrections and enhancements to manual mosaicking and corresponding to the Tunisian request for high resolution and the limited available funds. The scenes were processed by the Environmental Research Institute of Michigan, resampling the data geodesically corrected to fit the Universal Transverse Mercator projection using control points from topographic maps at 1:50,000 and 1:100,000 scales available in the U. S. The mosaicking was done in the Eastern Mapping Center under the supervision of the Graphic Arts System Section. The three black and white mosaics were made at the 1:1,000,000 scale and various products generated. They included color film positives at 1:2,000,000 and 1:4,000,000 scales reproducible in the Remote Sensing Laboratory in Tunis and corresponding color prints as well as tricolor prints at various scales from 1:500,000 to 1:2,000,000.

Recurrent SOX9 deletion campomelic dysplasia due to somatic mosaicism in the father.

Science.gov (United States)

Smyk, M; Obersztyn, E; Nowakowska, B; Bocian, E; Cheung, S W; Mazurczak, T; Stankiewicz, P

2007-04-15

Haploinsufficiency of SOX9, a master gene in chondrogenesis and testis development, leads to the semi-lethal skeletal malformation syndrome campomelic dysplasia (CD), with or without XY sex reversal. We report on two children with CD and a phenotypically normal father, a carrier of a somatic mosaic SOX9 deletion. This is the first report of a mosaic deletion of SOX9; few familial CD cases with germline and somatic mutation mosaicism have been described. Our findings confirm the utility of aCGH and indicate that for a more accurate estimate of the recurrence risk for a completely penetrant autosomal dominant disorder, parental somatic mosaicism should be considered in healthy parents. Copyright 2007 Wiley-Liss, Inc.

77 FR 42713 - Notice of Proposed Information Collection RequestsNPEFS 2011-2014: Common Core of Data (CCD...

Science.gov (United States)

2012-07-20

... Core of Data (CCD) National Public Education Financial Survey AGENCY: Institute of Education Sciences, Department of Education. SUMMARY: The National Public Education Financial Survey (NPEFS) is an annual... of Data (CCD) National Public Education Financial Survey. OMB Control Number: 1850-0067. [[Page 42714...

Development of stable monolithic wide-field Michelson interferometers.

Science.gov (United States)

Wan, Xiaoke; Ge, Jian; Chen, Zhiping

2011-07-20

Bulk wide-field Michelson interferometers are very useful for high precision applications in remote sensing and astronomy. A stable monolithic Michelson interferometer is a key element in high precision radial velocity (RV) measurements for extrasolar planets searching and studies. Thermal stress analysis shows that matching coefficients of thermal expansion (CTEs) is a critical requirement for ensuring interferometer stability. This requirement leads to a novel design using BK7 and LAK7 materials, such that the monolithic interferometer is free from thermal distortion. The processes of design, fabrication, and testing of interferometers are described in detail. In performance evaluations, the field angle is typically 23.8° and thermal sensitivity is typically -2.6×10(-6)/°C near 550 nm, which corresponds to ∼800 m/s/°C in the RV scale. Low-cost interferometer products have been commissioned in multiple RV instruments, and they are producing high stability performance over long term operations. © 2011 Optical Society of America

Noise in off-axis type holograms including reconstruction and CCD camera parameters

Energy Technology Data Exchange (ETDEWEB)

Voelkl, Edgar, E-mail: edgar.voelkl@fei.com [FEI Company, 5350 NE Dawson Creek Drive, Hillsboro, OR 97124-5793 (United States)

2010-02-15

Phase and amplitude images as contained in digital holograms are commonly extracted via a process called 'reconstruction'. Expressions for the expected noise in these images have been given in the past by several authors; however, the effect of the actual reconstruction process has not been fully appreciated. By starting with the Quantum Mechanical intensity distribution of the off-axis type interference pattern, then building the digital hologram on an electron-by-electron base while simultaneously reconstructing the phase/amplitude images and evaluating their noise levels, an expression is derived that consistently describes the noise in simulated and experimental phase/amplitude images and contains the reconstruction parameters. Because of the necessity to discretize the intensity distribution function, the digitization effects of an ideal CCD camera had to be included. Subsequently, this allowed a comparison between real and simulated holograms which then led to a comparison between the performance of an 'ideal' CCD camera versus a real device. It was concluded that significant improvement of the phase and amplitude noise may be obtained if CCD cameras were optimized for digitizing intensity distributions at low sampling rates.

Severe Outbreak of a Yellow Mosaic Disease on the Yard Long Bean in Bogor, West Java

Directory of Open Access Journals (Sweden)

TRI ASMIRA DAMAYANTI

2009-06-01

Full Text Available During 2008 crop season, an outbreak of severe yellow mosaic disease on yard long bean (Vigna unguiculata subsp. Sesquipedalis occurred in several farmers’ fields in West Java. Yard long bean var. Parade inoculated manually with extracts from symptomatic leaves showed the symptoms indicating the presence of virus. Symptomatic leaf samples tested positive in enzyme linked immunosorbent assay (ELISA with antibodies to group specific Potyvirus and Cucumber mosaic virus (CMV. Total RNA derived from symptomatic leaves was subjected to reverse transcription-polymerase chain reaction (RT-PCR using primers specific to the cylindrical inclusion (CI protein of potyviruses and CMV coat protein (CP specific primers. Pair wise comparison of sequences obtained from cloned RT-PCR products with corresponding nucleotide sequences in the GenBank confirmed the presence of Bean common mosaic virus strain Blackeye (BCMV-BlC and CMV in the symptomatic beans. Sequences of BCMV and CMV isolates from the beans showed maximum nucleotide sequence identities (92-97% and (90%, respectively with BCMV-BIC and CMV isolates from Taiwan. Each virus isolate also clustered closely with corresponding isolates from Taiwan in a phylogenetic analyses. These results provide first evidence of the occurrence of multiple infection of BCMV-BIC and CMV in the yard long been from Bogor, West Java.

Management of whitefly-transmitted viruses in open-field production systems.

Science.gov (United States)

Lapidot, Moshe; Legg, James P; Wintermantel, William M; Polston, Jane E

2014-01-01

Whiteflies are a key pest of crops in open-field production throughout the tropics and subtropics. This is due in large part to the long and diverse list of devastating plant viruses transmitted by these vectors. Open-field production provides many challenges to manage these viruses and in many cases adequate management has not been possible. Diseases caused by whitefly-transmitted viruses have become limiting factors in open-field production of a wide range of crops, i.e., bean golden mosaic disease in beans, tomato yellow leaf curl disease in tomato, cassava mosaic disease and cassava brown streak disease in cassava, and cotton leaf crumple disease in cotton. While host resistance has proven to be the most cost-effective management solution, few examples of host resistance have been developed to date. The main strategy to limit the incidence of virus-infected plants has been the application of insecticides to reduce vector populations aided to some extent by the use of selected cultural practices. However, due to concerns about the effect of insecticides on pollinators, consumer demand for reduced pesticide use, and the ability of the whitefly vectors to develop insecticide-resistance, there is a growing need to develop and deploy strategies that do not rely on insecticides. The reduction in pesticide use will greatly increase the need for genetic resistance to more viruses in more crop plants. Resistance combined with selected IPM strategies could become a viable means to increase yields in crops produced in open fields despite the presence of whitefly-transmitted viruses.

Development of network based control and data acquisition systems for diagnostics using CCD detectors. Application to LHD experiments

International Nuclear Information System (INIS)

Kado, Shinichiro; Nakanishi, Hideya; Ida, Katsumi; Kojima, Mamoru

2000-01-01

The needs of CCD detectors as a plasma diagnostic tool have recently been increased. However, many CCD providers have developed their own controlling systems, and it is difficult to customized the usages in order to make them applicable to the network-based data acquisition clusters which consist of various sorts of diagnostics. This paper presents the development of systems in which CCD detectors are controlled and the data are acquired through networks. By making use of the Client/Server (C/S) model in the Windows NT operating system and block transfer method via shared memory relevant to the model, the dependence on the hardware is hidden by the server service, CCD list sequencer. The client program is designed for the LHD (Large Helical Device) discharge operation sequences and the data acquisition system. (author)

Wide Field Radio Transient Surveys

Science.gov (United States)

Bower, Geoffrey

2011-04-01

The time domain of the radio wavelength sky has been only sparsely explored. Nevertheless, serendipitous discovery and results from limited surveys indicate that there is much to be found on timescales from nanoseconds to years and at wavelengths from meters to millimeters. These observations have revealed unexpected phenomena such as rotating radio transients and coherent pulses from brown dwarfs. Additionally, archival studies have revealed an unknown class of radio transients without radio, optical, or high-energy hosts. The new generation of centimeter-wave radio telescopes such as the Allen Telescope Array (ATA) will exploit wide fields of view and flexible digital signal processing to systematically explore radio transient parameter space, as well as lay the scientific and technical foundation for the Square Kilometer Array. Known unknowns that will be the target of future transient surveys include orphan gamma-ray burst afterglows, radio supernovae, tidally-disrupted stars, flare stars, and magnetars. While probing the variable sky, these surveys will also provide unprecedented information on the static radio sky. I will present results from three large ATA surveys (the Fly's Eye survey, the ATA Twenty CM Survey (ATATS), and the Pi GHz Survey (PiGSS)) and several small ATA transient searches. Finally, I will discuss the landscape and opportunities for future instruments at centimeter wavelengths.

LBA-ECO LC-15 JERS-1 Synthetic Aperture Radar, 1- km Mosaic, Amazon Basin: 1995-1996

Data.gov (United States)

National Aeronautics and Space Administration — This data set contains two image mosaics of L-band radar backscatter and two image mosaics of first order texture. The two backscatter images are mosaics of L-band...

Modeling energy fluxes in heterogeneous landscapes employing a mosaic approach

Science.gov (United States)

Klein, Christian; Thieme, Christoph; Priesack, Eckart

2015-04-01

Recent studies show that uncertainties in regional and global climate and weather simulations are partly due to inadequate descriptions of the energy flux exchanges between the land surface and the atmosphere. One major shortcoming is the limitation of the grid-cell resolution, which is recommended to be about at least 3x3 km² in most models due to limitations in the model physics. To represent each individual grid cell most models select one dominant soil type and one dominant land use type. This resolution, however, is often too coarse in regions where the spatial diversity of soil and land use types are high, e.g. in Central Europe. An elegant method to avoid the shortcoming of grid cell resolution is the so called mosaic approach. This approach is part of the recently developed ecosystem model framework Expert-N 5.0. The aim of this study was to analyze the impact of the characteristics of two managed fields, planted with winter wheat and potato, on the near surface soil moistures and on the near surface energy flux exchanges of the soil-plant-atmosphere interface. The simulated energy fluxes were compared with eddy flux tower measurements between the respective fields at the research farm Scheyern, North-West of Munich, Germany. To perform these simulations, we coupled the ecosystem model Expert-N 5.0 to an analytical footprint model. The coupled model system has the ability to calculate the mixing ratio of the surface energy fluxes at a given point within one grid cell (in this case at the flux tower between the two fields). This approach accounts for the differences of the two soil types, of land use managements, and of canopy properties due to footprint size dynamics. Our preliminary simulation results show that a mosaic approach can improve modeling and analyzing energy fluxes when the land surface is heterogeneous. In this case our applied method is a promising approach to extend weather and climate models on the regional and on the global scale.

Development of X-ray CCD camera system with high readout rate using ASIC

International Nuclear Information System (INIS)

Nakajima, Hiroshi; Matsuura, Daisuke; Anabuki, Naohisa; Miyata, Emi; Tsunemi, Hiroshi; Doty, John P.; Ikeda, Hirokazu; Katayama, Haruyoshi

2009-01-01

We report on the development of an X-ray charge-coupled device (CCD) camera system with high readout rate using application-specific integrated circuit (ASIC) and Camera Link standard. The distinctive ΔΣ type analog-to-digital converter is introduced into the chip to achieve effective noise shaping and to obtain a high resolution with relatively simple circuits. The unit test proved moderately low equivalent input noise of 70μV with a high readout pixel rate of 625 kHz, while the entire chip consumes only 100 mW. The Camera Link standard was applied for the connectivity between the camera system and frame grabbers. In the initial test of the whole system, we adopted a P-channel CCD with a thick depletion layer developed for X-ray CCD camera onboard the next Japanese X-ray astronomical satellite. The characteristic X-rays from 109 Cd were successfully read out resulting in the energy resolution of 379(±7)eV (FWHM) at 22.1 keV, that is, ΔE/E=1.7% with a readout rate of 44 kHz.

Somatic mosaicism underlies X-linked acrogigantism syndrome in sporadic male subjects.

Science.gov (United States)

Daly, Adrian F; Yuan, Bo; Fina, Frederic; Caberg, Jean-Hubert; Trivellin, Giampaolo; Rostomyan, Liliya; de Herder, Wouter W; Naves, Luciana A; Metzger, Daniel; Cuny, Thomas; Rabl, Wolfgang; Shah, Nalini; Jaffrain-Rea, Marie-Lise; Zatelli, Maria Chiara; Faucz, Fabio R; Castermans, Emilie; Nanni-Metellus, Isabelle; Lodish, Maya; Muhammad, Ammar; Palmeira, Leonor; Potorac, Iulia; Mantovani, Giovanna; Neggers, Sebastian J; Klein, Marc; Barlier, Anne; Liu, Pengfei; Ouafik, L'Houcine; Bours, Vincent; Lupski, James R; Stratakis, Constantine A; Beckers, Albert

2016-04-01

Somatic mosaicism has been implicated as a causative mechanism in a number of genetic and genomic disorders. X-linked acrogigantism (XLAG) syndrome is a recently characterized genomic form of pediatric gigantism due to aggressive pituitary tumors that is caused by submicroscopic chromosome Xq26.3 duplications that include GPR101 We studied XLAG syndrome patients (n= 18) to determine if somatic mosaicism contributed to the genomic pathophysiology. Eighteen subjects with XLAG syndrome caused by Xq26.3 duplications were identified using high-definition array comparative genomic hybridization (HD-aCGH). We noted that males with XLAG had a decreased log2ratio (LR) compared with expected values, suggesting potential mosaicism, whereas females showed no such decrease. Compared with familial male XLAG cases, sporadic males had more marked evidence for mosaicism, with levels of Xq26.3 duplication between 16.1 and 53.8%. These characteristics were replicated using a novel, personalized breakpoint junction-specific quantification droplet digital polymerase chain reaction (ddPCR) technique. Using a separate ddPCR technique, we studied the feasibility of identifying XLAG syndrome cases in a distinct patient population of 64 unrelated subjects with acromegaly/gigantism, and identified one female gigantism patient who had had increased copy number variation (CNV) threshold for GPR101 that was subsequently diagnosed as having XLAG syndrome on HD-aCGH. Employing a combination of HD-aCGH and novel ddPCR approaches, we have demonstrated, for the first time, that XLAG syndrome can be caused by variable degrees of somatic mosaicism for duplications at chromosome Xq26.3. Somatic mosaicism was shown to occur in sporadic males but not in females with XLAG syndrome, although the clinical characteristics of the disease were similarly severe in both sexes. © 2016 Society for Endocrinology.

Detection of somatic mosaicism in DMD using computer-assisted laser densitometry

Energy Technology Data Exchange (ETDEWEB)

Sutherland, J.E.; Allingham-Hawkins, D.J.; MacKenzie, J. [Hospital for Sick Children, Toronto (Canada)] [and others

1994-09-01

Approximately two-thirds of Duchenne muscular dystrophy (DMD) patients have a deletion in the dystrophin gene located at Xp21.1. Two PCR-based multiplex systems have been developed which detect 98% of deletions in affected males. Diagnosis of carrier females requires densitometry of PCR products following gel electrophoresis to calculate dosage of specific exons. We have developed a system in which fluorescently labelled PCR products are analysed using a GENESCANNER automated fragment analyser (ABI). Dosage is determined using computer-assisted laser densitometry (CALD). Recently, we diagnosed somatic mosaicism in the mother of an affected boy using this method. PCR analysis showed that the patient had a deletion that included exons 47-51 of his dystrophin gene. CALD analysis on the patient`s 36-year-old mother revealed a 29-34% reduction in the intensity of the bands corresponding to the deleted region of the gene rather than the 50% reduction normally seen in carrier females. A skin biopsy was obtain and monoclonal fibroblast colonies were tested by CALD for the deletion. Four of the twenty colonies screened were found to be deleted while the remaining colonies had two intact copies of the gene. We conclude that this patient is a somatic mosaic for DMD and that the mutation was the result of a post-zygotic event. This is the only case of somatic mosaicism detected among 800 women from 400 DMD families tested using CALD in our laboratory. At least one other case of possible somatic mosaicism has been reported but not confirmed. Germinal mosaicism is thought to occur in approximately 10% of mothers of sporadic DMD patients. Our findings indicate that somatic mosaicism is a much rarer condition among DMD carriers, thus suggesting that mitotic mutations in the dystrophin gene are more likely to occur later in embryogenesis after differentiation of the germline.

Resistance Evaluation of Radish (Raphanus sativus L. Inbred Lines against Turnip mosaic virus

Directory of Open Access Journals (Sweden)

Ju-Yeon Yoon

2017-03-01

Full Text Available Leaves of twenties radish (Raphanus sativus L. inbred lines were mechanically inoculated with Turnip mosaic virus (TuMV strain HY to evaluate TuMV resistance of the radish inbred lines. The inoculated radish plants were incubated at 22°C±3°C and resistance assessment was examined using symptom development for 4 weeks. Based on the reactions of differential radish inbred lines, 16 radish lines were produced mild mosaic, mottling, mosaic and severe mosaic symptoms by TuMV infection. These results were confirmed by RT-PCR analysis of TuMV coat protein gene, suggesting that TuMV is responsible for the disease symptoms. Four resistant radish lines did not induce systemic mosaic symptoms on upper leaves and chlorosis in stem tissues for 4 weeks, showing they were symptomless by 8 weeks. Further examination of TuMV infection in the 4 radish lines showed no TuMV infection in all systemic leaves. These results suggest that the 4 radish lines are highly resistant to TuMV.

VizieR Online Data Catalog: Isaac Newton Telescope Wide Field Survey (CASU 2002)

Science.gov (United States)

Cambridge Astronomical Survey Unit

2002-04-01

The INT Wide Field Survey (WFS) is using the Wide Field Camera (~0.3 square degrees) on the 2.5m Isaac Newton Telescope (INT). The project was initiated in August 1998 and is expected to have a duration of up to five years. Multicolour data will be obtained over 200+ square degrees to a typical depth of ~25 mag (u' through z'). The data is publically accessible via the Cambridge Astronomical Survey Unit to UK and NL communities from day one, with access to the rest of the world after one year. This observation log lists all observations older than the one year proprietary period. (1 data file).

The Cross-Cultural Dementia Screening (CCD): A new neuropsychological screening instrument for dementia in elderly immigrants.

Science.gov (United States)

Goudsmit, Miriam; Uysal-Bozkir, Özgül; Parlevliet, Juliette L; van Campen, Jos P C M; de Rooij, Sophia E; Schmand, Ben

2017-03-01

Currently, approximately 3.9% of the European population are non-EU citizens, and a large part of these people are from "non-Western" societies, such as Turkey and Morocco. For various reasons, the incidence of dementia in this group is expected to increase. However, cognitive testing is challenging due to language barriers and low education and/or illiteracy. The newly developed Cross-Cultural Dementia Screening (CCD) can be administered without an interpreter. It contains three subtests that assess memory, mental speed, and executive function. We hypothesized the CCD to be a culture-fair test that could discriminate between demented patients and cognitively healthy controls. To test this hypothesis, 54 patients who had probable dementia were recruited via memory clinics. Controls (N = 1625) were recruited via their general practitioners. All patients and controls were aged 55 years and older and of six different self-defined ethnicities (Dutch, Turkish, Moroccan-Arabic, Moroccan-Berber, Surinamese-Creole, and Surinamese-Hindustani). Exclusion criteria included current or previous conditions that affect cognitive functioning. There were performance differences between the ethnic groups, but these disappeared after correcting for age and education differences between the groups, which supports our central hypothesis that the CCD is a culture-fair test. Receiver-operating characteristic (ROC) and logistic regression analyses showed that the CCD has high predictive validity for dementia (sensitivity: 85%; specificity: 89%). The CCD is a sensitive and culture-fair neuropsychological instrument for dementia screening in low-educated immigrant populations.

Fast event recorder utilizing a CCD analog shift register

International Nuclear Information System (INIS)

Ducar, R.J.; McIntyre, P.M.

1978-01-01

A system of electronics has been developed to allow the capture and recording of relatively fast, low-amplitude analog events. The heart of the system is a dual 455-cell analog shift register charge-coupled device, Fairchild CCD321ADC-3. The CCD is operated in a dual clock mode. The input is sampled at a selectable clock rate of .25-20 MHz. The stored analog data is then clocked out at a slower rate, typically about .25 MHz. The time base expansion of the analog data allows for analog-to-digital conversion and memory storage using conventional medium-speed devices. The digital data is sequentially loaded into a static RAM and may then be block transferred to a computer. The analog electronics are housed in a single-width NIM module, and the RAM memory in a single-width CAMAC module. Each pair of modules provides six parallel channels. Cost is about $200.00 per channel. Applications are described for ionization imaging (TPC, IRC) and long-drift calorimetry in liquid argon

First Light with a 67-Million-Pixel WFI Camera

Science.gov (United States)

1999-01-01

optical astronomical instruments - the "Charge-Coupled Devices (CCD's)" - are currently restricted to about 4000 x 4000 pixels. For the time being, the only possible way towards even larger detector areas is by assembling mosaics of CCD's. ESO , MPI-A and OAC have therefore undertaken a joint project to build a new and large astronomical camera with a mosaic of CCD's. This new Wide Field Imager (WFI) comprises eight CCD's with high sensitivity from the ultraviolet to the infrared spectral domain, each with 2046 x 4098 pixels. Mounted behind an advanced optical system at the Cassegrain focus of the 2.2-m telescope of the Max-Planck-Gesellschaft (MPG) at ESO's La Silla Observatory in Chile, the combined 8184 x 8196 = 67,076,064 pixels cover a square field-of-view with an edge of more than half a degree (over 30 arcmin) [1]. Compared to the viewing field of the human eye, this may still appear small, but in the domain of astronomical instrumentation, it is indeed a large step forward. For comparison, the largest field-of-view with the FORS1 instrument at the VLT is about 7 arcmin. Moreover, the level of detail detectable with the WFI (theoretical image sharpness) exceeds what is possible with the naked eye by a factor of about 10,000. The WFI project was completed in only two years in response to a recommendation to ESO by the "La Silla 2000" Working Group and the Scientific-Technical Committee (STC) to offer this type of instrument to the community. The MPI-A proposed to build such an instrument for the MPG/ESO 2.2-m telescope and a joint project was soon established. A team of astronomers from the three institutions is responsible for the initial work with the WFI at La Silla. A few other Cameras of this size are available, e.g. at Hawaii, Kitt Peak (USA) and Cerro Tololo (Chile), but this is the first time that a telescope this large has been fully dedicated to wide-field imaging with an 8kx8k CCD. The first WFI images Various exposures were obtained during the early

The possibilities of CCD photometry of optical afterglows of GRBs

Czech Academy of Sciences Publication Activity Database

Šimon, Vojtěch; Polášek, Cyril; Jelínek, M.; Hudec, René; Štrobl, Jan

-, č. 125 (2010), s. 24-28 ISSN 1801-5964. [Conference on Variable Stars Research /41./. Prague, 27.11.2009-29.11.2009] Institutional research plan: CEZ:AV0Z10030501 Keywords : gamma-ray bursts * optical afterglows * CCD photometry Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics

Bypass of lethality with mosaic mice generated by Cre-loxP-mediated recombination.

Science.gov (United States)

Betz, U A; Vosshenrich, C A; Rajewsky, K; Müller, W

1996-10-01

The analysis of gene function based on the generation of mutant mice by homologous recombination in embryonic stem cells is limited if gene disruption results in embryonic lethality. Mosaic mice, which contain a certain proportion of mutant cells in all organs, allow lethality to be circumvented and the potential of mutant cells to contribute to different cell lineages to be analyzed. To generate mosaic animals, we used the bacteriophage P1-derived Cre-loxP recombination system, which allows gene alteration by Cre-mediated deletion of loxP-flanked gene segments. We generated nestin-cre transgenic mouse lines, which expressed the Cre recombinase under the control of the rat nestin promoter and its second intron enhancer. In crosses to animals carrying a loxP-flanked target gene, partial deletion of the loxP-flanked allele occurred before day 10.5 post coitum and was detectable in all adult organs examined, including germ-line cells. Using this approach, we generated mosaic mice containing cells deficient in the gamma-chain of the interleukin-2 receptor (IL-2R gamma); in these animals, the IL-2R gamma-deficient cells were underrepresented in the thymus and spleen. Because mice deficient in DNA polymerase beta die perinatally, we studied the effects of DNA polymerase beta deficiency in mosaic animals. We found that some of the mosaic polymerase beta-deficient animals were viable, but were often reduced in size and weight. The fraction of DNA polymerase beta-deficient cells in mosaic embryos decreased during embryonic development, presumably because wild-type cells had a competitive advantage. The nestin-cre transgenic mice can be used to generate mosaic animals in which target genes are mutated by Cre-mediated recombination of loxP-flanked target genes. By using mosaic animals, embryonic lethality can be bypassed and cell lineages for whose development a given target gene is critical can be identified. In the case of DNA polymerase beta, deficient cells are already

Cowpea Mosaic Virus-Encoded Protease Does Not Recognize Primary Translation Products of M RNAs from Other Comoviruses

OpenAIRE

Goldbach, Rob; Krijt, Jette

1982-01-01

The protease encoded by the large (B) RNA segment of cowpea mosaic virus was tested for its ability to recognize the in vitro translation products of the small (M) RNA segment from the comoviruses squash mosaic virus, red clover mottle virus, and cowpea severe mosaic virus (CPsMV, strains Dg and Ark), and from the nepovirus tomato black ring virus. Like M RNA from cowpea mosaic virus, the M RNAs from squash mosaic virus, red clover mottle virus, CPsMV-Dg, and CPsMV-Ark were all translated int...

Extending Driving Vision Based on Image Mosaic Technique

Directory of Open Access Journals (Sweden)

Chen Deng

2017-01-01

Full Text Available Car cameras have been used extensively to assist driving by make driving visible. However, due to the limitation of the Angle of View (AoV, the dead zone still exists, which is a primary origin of car accidents. In this paper, we introduce a system to extend the vision of drivers to 360 degrees. Our system consists of four wide-angle cameras, which are mounted at different sides of a car. Although the AoV of each camera is within 180 degrees, relying on the image mosaic technique, our system can seamlessly integrate 4-channel videos into a panorama video. The panorama video enable drivers to observe everywhere around a car as far as three meters from a top view. We performed experiments in a laboratory environment. Preliminary results show that our system can eliminate vision dead zone completely. Additionally, the real-time performance of our system can satisfy requirements for practical use.

Mosaic-shaped cathode for highly durable solid oxide fuel cell under thermal stress

Science.gov (United States)

Joo, Jong Hoon; Jeong, Jaewon; Kim, Se Young; Yoo, Chung-Yul; Jung, Doh Won; Park, Hee Jung; Kwak, Chan; Yu, Ji Haeng

2014-02-01

In this study, we propose a novel "mosaic structure" for a SOFC (solid oxide fuel cell) cathode with high thermal expansion to improve the stability against thermal stress. Self-organizing mosaic-shaped cathode has been successfully achieved by controlling the amount of binder in the dip-coating solution. The anode-supported cell with mosaic-shaped cathode shows itself to be highly durable performance for rapid thermal cycles, however, the performance of the cell with a non-mosaic cathode exhibits severe deterioration originated from the delamination at the cathode/electrolyte interface after 7 thermal cycles. The thermal stability of an SOFC cathode can be evidently improved by controlling the surface morphology. In view of the importance of the thermal expansion properties of the cathode, the effects of cathode morphology on the thermal stress stability are discussed.

BVRI CCD photometry of the globular cluster NGC 2808

International Nuclear Information System (INIS)

Alcaino, G.; Liller, W.; Alvarado, F.; Wenderoth, E.

1990-01-01

As a part of a continuing program, CCD color-magnitude diagrams are presented for the bright globular cluster NGC 2808 in the four colors comprising BVRI. From a comparison of four different CMDs with theoretical isochrones, an age of 16 + or - 2 Gyr is obtained, assuming a value for Fe/H near -1.3. 28 refs

Wide-field Spatio-Spectral Interferometry: Bringing High Resolution to the Far- Infrared

Science.gov (United States)

Leisawitx, David

Wide-field spatio-spectral interferometry combines spatial and spectral interferometric data to provide integral field spectroscopic information over a wide field of view. This technology breaks through a mission cost barrier that stands in the way of resolving spatially and measuring spectroscopically at far-infrared wavelengths objects that will lead to a deep understanding of planetary system and galaxy formation processes. A space-based far-IR interferometer will combine Spitzer s superb sensitivity with a two order of magnitude gain in angular resolution, and with spectral resolution in the thousands. With the possible exception of detector technology, which is advancing with support from other research programs, the greatest challenge for far-IR interferometry is to demonstrate that the interferometer will actually produce the images and spectra needed to satisfy mission science requirements. With past APRA support, our team has already developed the highly specialized hardware testbed, image projector, computational model, and image construction software required for the proposed effort, and we have access to an ideal test facility.

A comparison of film and 3 digital imaging systems for natural dental caries detection: CCD, CMOS, PSP and film

Energy Technology Data Exchange (ETDEWEB)

Han, Won Jeong [Dankook University College of Medicine, Seoul (Korea, Republic of)

2004-03-15

To evaluate the diagnostic accuracy of occlusal and proximal caries detection using CCD, CMOS, PSP and film system. 32 occlusal and 30 proximal tooth surfaces were radiographed under standardized conditions using 3 digital systems; CCD (CDX-2000HQ, Biomedysis Co., Seoul, Korea), CMOS (Schick, Schick Inc., Long Island, USA), PSP (Digora FMX, Orion Co./Soredex, Helsinki, Finland) and 1 film system (Kodak Insight, Eastman Kodak, Rochester, USA). 5 observers examined the radiographs for occlusal and proximal caries using a 5-point confidence scale. The presence of caries was validated histologically and radiographically. Diagnostic accuracy was evaluated using ROC curve areas (AZ). Analysis using ROC curves revealed the area under each curve which indicated a diagnostic accuracy. For occlusal caries, Kodak Insight film had an Az of 0.765, CCD one of 0.730, CMOS one of 0.742 and PSP one of 0.735. For proximal caries, Kodak Insight film had an Az of 0.833, CCD one of 0.832, CMOS one of 0.828 and PSP one of 0.868. No statistically significant difference was noted between any of the imaging modalities. CCD, CMOS, PSP and film performed equally well in the detection of occlusal and proximal dental caries. CCD, CMOS and PSP-based digital images provided a level of diagnostic performance comparable to Kodak Insight film.

A comparison of film and 3 digital imaging systems for natural dental caries detection: CCD, CMOS, PSP and film

International Nuclear Information System (INIS)

Han, Won Jeong

2004-01-01

To evaluate the diagnostic accuracy of occlusal and proximal caries detection using CCD, CMOS, PSP and film system. 32 occlusal and 30 proximal tooth surfaces were radiographed under standardized conditions using 3 digital systems; CCD (CDX-2000HQ, Biomedysis Co., Seoul, Korea), CMOS (Schick, Schick Inc., Long Island, USA), PSP (Digora FMX, Orion Co./Soredex, Helsinki, Finland) and 1 film system (Kodak Insight, Eastman Kodak, Rochester, USA). 5 observers examined the radiographs for occlusal and proximal caries using a 5-point confidence scale. The presence of caries was validated histologically and radiographically. Diagnostic accuracy was evaluated using ROC curve areas (AZ). Analysis using ROC curves revealed the area under each curve which indicated a diagnostic accuracy. For occlusal caries, Kodak Insight film had an Az of 0.765, CCD one of 0.730, CMOS one of 0.742 and PSP one of 0.735. For proximal caries, Kodak Insight film had an Az of 0.833, CCD one of 0.832, CMOS one of 0.828 and PSP one of 0.868. No statistically significant difference was noted between any of the imaging modalities. CCD, CMOS, PSP and film performed equally well in the detection of occlusal and proximal dental caries. CCD, CMOS and PSP-based digital images provided a level of diagnostic performance comparable to Kodak Insight film.

Design, manufacturing and testing of a four-mirror telescope with a wide field of view

Science.gov (United States)

Gloesener, P.; Wolfs, F.; Lemagne, F.; Cola, M.; Flebus, C.; Blanchard, G.; Kirschner, V.

2017-11-01

Regarding Earth observation missions, it has become unnecessary to point out the importance of making available wide field of view optical instruments for the purpose of spectral imaging. Taking advantage of the pushbroom instrument concept with its linear field across the on-ground track, it is in particular relevant to consider front-end optical configurations that involve an all-reflective system presenting inherent and dedicated advantages such as achromaticity, unobscuration and compactness, while ensuring the required image quality over the whole field. The attractiveness of the concept must be balanced with respect to the state-of-the-art mirror manufacturing technologies as the need for fast, broadband and wide field systems increases the constraints put on the feasibility of each individual component. As part of an ESTEC contract, AMOS designed, manufactured and tested a breadboard of a four-mirror wide field telescope for typical Earth observation superspectral missions. The initial purpose of the development was to assess the feasibility of a telecentric spaceborne three-mirror system covering an unobscured rectangular field of view of 26 degrees across track (ACT) by 6 degrees along track (ALT) with a f-number of 3.5 and a focal length of 500 mm and presenting an overall image quality better than 100 nm RMS wavefront error within the whole field.

Measuring a narrow Bessel beam spot by scanning a charge-coupled device (CCD) pixel

International Nuclear Information System (INIS)

Tiwari, S K; Ram, S P; Jayabalan, J; Mishra, S R

2010-01-01

By scanning a charge-coupled device (CCD) camera transverse to the beam axis and observing the variation in counts on a marked pixel, we demonstrate that we can measure a laser beam spot size smaller than the size of the CCD-pixel. We find this method particularly attractive for measuring the size of central spot of a Bessel beam, for which the established scanning knife-edge method does not work appropriately because of the large contribution of the rings surrounding the central spot to the signal

A Wide Field Auroral Imager (WFAI for low Earth orbit missions

Directory of Open Access Journals (Sweden)

N. P. Bannister

2007-03-01

Full Text Available A comprehensive understanding of the solar wind interaction with Earth's coupled magnetosphere-ionosphere system requires an ability to observe the charged particle environment and auroral activity from the same platform, generating particle and photon image data which are matched in time and location. While unambiguous identification of the particles giving rise to the aurora requires a Low Earth Orbit satellite, obtaining adequate spatial coverage of aurorae with the relatively limited field of view of current space bourne auroral imaging systems requires much higher orbits. A goal for future satellite missions, therefore, is the development of compact, wide field-of-view optics permitting high spatial and temporal resolution ultraviolet imaging of the aurora from small spacecraft in low polar orbit. Microchannel plate optics offer a method of achieving the required performance. We describe a new, compact instrument design which can observe a wide field-of-view with the required spatial resolution. We report the focusing of 121.6 nm radiation using a spherically-slumped, square-pore microchannel plate with a focal length of 32 mm and an F number of 0.7. Measurements are compared with detailed ray-trace simulations of imaging performance. The angular resolution is 2.7±0.2° for the prototype, corresponding to a footprint ~33 km in diameter for an aurora altitude of 110 km and a spacecraft altitude of 800 km. In preliminary analysis, a more recent optic has demonstrated a full width at half maximum of 5.0±0.3 arcminutes, corresponding to a footprint of ~1 km from the same spacecraft altitude. We further report the imaging properties of a convex microchannel plate detector with planar resistive anode readout; this detector, whose active surface has a radius of curvature of only 100 mm, is shown to meet the spatial resolution and sensitivity requirements of the new wide field auroral imager (WFAI.

Cowpea Mosaic Virus-Encoded Protease Does Not Recognize Primary Translation Products of M RNAs from Other Comoviruses.

Science.gov (United States)

Goldbach, R; Krijt, J

1982-09-01

The protease encoded by the large (B) RNA segment of cowpea mosaic virus was tested for its ability to recognize the in vitro translation products of the small (M) RNA segment from the comoviruses squash mosaic virus, red clover mottle virus, and cowpea severe mosaic virus (CPsMV, strains Dg and Ark), and from the nepovirus tomato black ring virus. Like M RNA from cowpea mosaic virus, the M RNAs from squash mosaic virus, red clover mottle virus, CPsMV-Dg, and CPsMV-Ark were all translated into two large polypeptides with apparent molecular weights which were different for each virus and even for the two CPsMV strains. Neither the in vitro products from squash mosaic virus, red clover mottle virus, and CPsMV M RNAs nor the in vitro product from tomato black ring virus RNA-2 were processed by the cowpea mosaic virus-encoded protease, indicating that the activity of this enzyme is highly specific.

Complete trisomy 14 mosaicism: first live-born case in Korea

Directory of Open Access Journals (Sweden)

Yun Jung Hur

2012-10-01

Full Text Available Trisomy 14 mosaicism is a rare chromosome disorder characterized by delayed development, failure to thrive, and facial dysmorphism. Only approximately 30 trisomy 14 mosaicism cases have been reported in the literature because trisomy 14 is associated with early spontaneous abortion. We report a case of a 17-month-old girl with abnormal skin pigmentation, delayed development, facial dysmorphism, and failure to thrive with the 47,XX,+14/46,XX chromosome complement.

BECN2 interacts with ATG14 through a metastable coiled-coil to mediate autophagy: BECN2 CCD Structure and Interaction with ATG14

Energy Technology Data Exchange (ETDEWEB)

Su, Minfei; Li, Yue; Wyborny, Shane; Neau, David; Chakravarthy, Srinivas; Levine, Beth; Colbert, Christopher L.; Sinha, Sangita C. (NDSU); (IIT); (Cornell); (UTSMC)

2017-03-12

ATG14 binding to BECN/Beclin homologs is essential for autophagy, a critical catabolic homeostasis pathway. Here, we show that the α-helical, coiled-coil domain (CCD) of BECN2, a recently identified mammalian BECN1 paralog, forms an antiparallel, curved homodimer with seven pairs of nonideal packing interactions, while the BECN2 CCD and ATG14 CCD form a parallel, curved heterodimer stabilized by multiple, conserved polar interactions. Compared to BECN1, the BECN2 CCD forms a weaker homodimer, but binds more tightly to the ATG14 CCD. Mutation of nonideal BECN2 interface residues to more ideal pairs improves homodimer self-association and thermal stability. Unlike BECN1, all BECN2 CCD mutants bind ATG14, although more weakly than wild type. Thus, polar BECN2 CCD interface residues result in a metastable homodimer, facilitating dissociation, but enable better interactions with polar ATG14 residues stabilizing the BECN2:ATG14 heterodimer. These structure-based mechanistic differences in BECN1 and BECN2 homodimerization and heterodimerization likely dictate competitive ATG14 recruitment.

Prenatal diagnosis and gonadal findings in X/XXX mosaicism.

Science.gov (United States)

Kohn, G; Cohen, M M; Beyth, Y; Ornoy, A

1977-01-01

Prenatal diagnosis of a case of X/XXX mosaicism is presented. In spite of the fact that over 50% of the cells cultured from both ovaries were trisomic for the X chromosome, fetal öocytes were rarely found. This case illustrates that the presence of a triple-X cell line, even in a relatively high percentage of ovarian cells, does not necessarily protect the ovary from 'aöogenesis'. This observation might prove useful in the counselling of future cases involving the prenatal detection of sex chromosome mosaicism. Images PMID:856232

CCD Photometry Using Multiple Comparison Stars

Directory of Open Access Journals (Sweden)

Yonggi Kim

2004-09-01

Full Text Available The accuracy of CCD observations obtained at the Korean 1.8 m telescope has been studied. Seventeen comparison stars in the vicinity of the cataclysmic variable BG CMi have been measured. The ``artificial" star has been used instead of the ``control" star, what made possible to increase accuracy estimates by a factor of 1.3-2.1 times for ``good" and ``cloudy" nights, respectively. The algorithm of iterative determination of accuracy and weights of few comparison stars contributing to the artificial star, has been presented. The accuracy estimates for 13-mag stars are around 0.002 m mag for exposure times of 30 sec.

Measuring high-resolution sky luminance distributions with a CCD camera.

Science.gov (United States)

Tohsing, Korntip; Schrempf, Michael; Riechelmann, Stefan; Schilke, Holger; Seckmeyer, Gunther

2013-03-10

We describe how sky luminance can be derived from a newly developed hemispherical sky imager (HSI) system. The system contains a commercial compact charge coupled device (CCD) camera equipped with a fish-eye lens. The projection of the camera system has been found to be nearly equidistant. The luminance from the high dynamic range images has been calculated and then validated with luminance data measured by a CCD array spectroradiometer. The deviation between both datasets is less than 10% for cloudless and completely overcast skies, and differs by no more than 20% for all sky conditions. The global illuminance derived from the HSI pictures deviates by less than 5% and 20% under cloudless and cloudy skies for solar zenith angles less than 80°, respectively. This system is therefore capable of measuring sky luminance with the high spatial and temporal resolution of more than a million pixels and every 20 s respectively.

Effect of medicinal plants extracts on the incidence of mosaic disease caused by cucumber mosaic virus and growth of chili

Science.gov (United States)

Hamidson, H.; Damiri, N.; Angraini, E.

2018-01-01

This research was conducted to study the effect of the application of several extracts of medicinal plants on the incidence of mosaic disease caused by Cucumber Mosaic Virus infection on the chili (Capsicum annuum L.) plantation. A Randomized Block Design with eight treatments including control was used throughout the experiment. Treatments consisted of Azadiracta indica (A), Piper bitle (B), Cymbopogon citrates (C), Curcuma domestica (D), Averroa bilimbi (E), Datura stramonium (F), Annona Muricata (G) and control (H). Each treatment consist of three replications. The parameters observed were the incidence of mosaic attack due to CMV, disease severity, plant height, wet and dry weight and production (number of fruits and the weight of total fruits) each plant. Results showed that the application of medicinal plant extracts reduced the disease severity due to CMV. Extracts of Annona muricata and Datura stramonium were most effective in suppressing disease severity caused by the virus as they significantly different from control and from a number of treatment. The plants medicinal extracts were found to have increased the plant height and total weight of the plant, fruit amount and fruit weight. Extracts of Curcuma domestica, Piper bitle and Cymbopogon citrates were the third highest in fruit amount and weight and significantly different from the control.

Imaging design of the wide field x-ray monitor onboard the HETE satellite

International Nuclear Information System (INIS)

Zand, J.J.M. In'T; Fenimore, E.E.; Kawai, N.; Yoshida, A.; Matsuoka, M.; Yamauchi, M.

1994-01-01

The High Energy Transient Experiment (HETE), to be launched in 1995, will study Gamma-Ray Bursts in an unprecendented wide wavelength range from Gamma- and X-ray to UV wavelengths. The X-ray range (2 to 25 keV) will be covered by 2 perpendicularly oriented 1-dimensional coded aperture cameras. These instruments cover a wide field of view of 2 sr and thus have a relatively large potential to locate GRBs to a fraction of a degree, which is an order of magnitude better than BATSE. The imaging design of these coded aperture cameras relates to the design of the coded apertures and the decoding algorithm. The aperture pattern is to a large extent determined by the high background in this wide field application and the low number of pattern elements (∼100) in each direction. The result is a random pattern with an open fraction of 33%. The onboard decoding algorithm is dedicated to the localization of a single point source

Real-time construction and visualisation of drift-free video mosaics from unconstrained camera motion

Directory of Open Access Journals (Sweden)

Mateusz Brzeszcz

2015-08-01

Full Text Available This work proposes a novel approach for real-time video mosaicking facilitating drift-free mosaic construction and visualisation, with integrated frame blending and redundancy management, that is shown to be flexible to a range of varying mosaic scenarios. The approach supports unconstrained camera motion with in-sequence loop closing, variation in camera focal distance (zoom and recovery from video sequence breaks. Real-time performance, over extended duration sequences, is realised via novel aspects of frame management within the mosaic representation and thus avoiding the high data redundancy associated with temporally dense, spatially overlapping video frame inputs. This managed set of image frames is visualised in real time using a dynamic mosaic representation of overlapping textured graphics primitives in place of the traditional globally constructed, and hence frequently reconstructed, mosaic image. Within this formulation, subsequent optimisation occurring during online construction can thus efficiency adjust relative frame positions via simple primitive position transforms. Effective visualisation is similarly facilitated by online inter-frame blending to overcome the illumination and colour variance associated with modern camera hardware. The evaluation illustrates overall robustness in video mosaic construction under a diverse range of conditions including indoor and outdoor environments, varying illumination and presence of in-scene motion on varying computational platforms.

Development and characterization of a CCD camera system for use on six-inch manipulator systems

International Nuclear Information System (INIS)

Logory, L.M.; Bell, P.M.; Conder, A.D.; Lee, F.D.

1996-01-01

The Lawrence Livermore National Laboratory has designed, constructed, and fielded a compact CCD camera system for use on the Six Inch Manipulator (SIM) at the Nova laser facility. The camera system has been designed to directly replace the 35 mm film packages on all active SIM-based diagnostics. The unit's electronic package is constructed for small size and high thermal conductivity using proprietary printed circuit board technology, thus reducing the size of the overall camera and improving its performance when operated within the vacuum environment of the Nova laser target chamber. The camera has been calibrated and found to yield a linear response, with superior dynamic range and signal-to-noise levels as compared to T-Max 3200 optic film, while providing real-time access to the data. Limiting factors related to fielding such devices on Nova will be discussed, in addition to planned improvements of the current design

Left-sided congenital heart lesions in mosaic Turner syndrome.

Science.gov (United States)

Bouayed Abdelmoula, Nouha; Abdelmoula, Balkiss; Smaoui, Walid; Trabelsi, Imen; Louati, Rim; Aloulou, Samir; Aloulou, Wafa; Abid, Fatma; Kammoun, Senda; Trigui, Khaled; Bedoui, Olfa; Denguir, Hichem; Mallek, Souad; Ben Aziza, Mustapha; Dammak, Jamila; Kaabi, Oldez; Abdellaoui, Nawel; Turki, Fatma; Kaabi, Asma; Kamoun, Wafa; Jabeur, Jihen; Ltaif, Wided; Chaker, Kays; Fourati, Haytham; M'rabet, Samir; Ben Ameur, Hedi; Gouia, Naourez; Mhiri, Mohamed Nabil; Rebai, Tarek

2018-04-01

In the era of the diseasomes and interactome networks, linking genetics with phenotypic traits in Turner syndrome should be studied thoroughly. As a part of this stratagem, mosaicism of both X and Y chromosome which is a common finding in TS and an evaluation of congenital heart diseases in the different situations of mosaic TS types, can be helpful in the identification of disturbed sex chromosomes, genes and signaling pathway actors. Here we report the case of a mosaic TS associated to four left-sided CHD, including BAV, COA, aortic aneurysms and dissections at an early age. The mosaicism included two cell lines, well-defined at the cytogenetic and molecular levels: a cell line which is monosomic for Xp and Xq genes (45,X) and another which is trisomic for pseudoautosomal genes that are present on the X and Y chromosomes and escape X inactivation: 45,X[8]/46,X,idic(Y)(pter→q11.2::q11.2→pter)[42]. This case generates two hypotheses about the contribution of genes linked to the sex chromosomes and the signaling pathways involving these genes, in left-sided heart diseases. The first hypothesis suggests the interaction between X chromosome and autosomal genes or loci of aortic development, possibly dose-dependent, and which could be in the framework of TGF-β-SMAD signaling pathways. The second implies that left-sided congenital heart lesions involve sex chromosomes loci. The reduced dosage of X chromosome gene(s), escaping X inactivation during development, contributes to this type of CHD. Regarding our case, these X chromosome genes may have homologues at the Y chromosome, but the process of inactivation of the centromeres of the isodicentric Y spreads to the concerned Y chromosome genes. Therefore, this case emerges as an invitation to consider the mosaics of Turner syndrome and to study their phenotypes in correlation with their genotypes to discover the underlying developmental and genetic mechanisms, especially the ones related to sex chromosomes.

Immunocapture RT-PCR detection of Bean common mosaic virus and strain blackeye cowpea mosaic in common bean and black gram in India

DEFF Research Database (Denmark)

Udayashankar, A.C.; Nayaka, S. Chandra; Niranjana, S.R.

2012-01-01

The strains of Bean common mosaic virus (BCMV) and blackeye cowpea mosaic (BICM), genus Potyvirus, were detected from 25 common bean and 14 black gram seeds among 142 seed samples collected from different legume-growing regions of India. The samples were subjected to a growing-on test, an indicator...... plant test, an electron microscopic observations, an enzyme linked immunosorbent assay and an immunocapture RT-PCR. The incidence of the two tested viruses in common bean and black gram seed samples was 1–6% and 0.5–3.5%, respectively in growing-on test evaluations. Electron microscopic observations...

UNUSUALLY WIDE BINARIES: ARE THEY WIDE OR UNUSUAL?

International Nuclear Information System (INIS)

Kraus, Adam L.; Hillenbrand, Lynne A.

2009-01-01

We describe an astrometric and spectroscopic campaign to confirm the youth and association of a complete sample of candidate wide companions in Taurus and Upper Sco. Our survey found 15 new binary systems (three in Taurus and 12 in Upper Sco) with separations of 3''-30'' (500-5000 AU) among all of the known members with masses of 2.5-0.012 M sun . The total sample of 49 wide systems in these two regions conforms to only some expectations from field multiplicity surveys. Higher mass stars have a higher frequency of wide binary companions, and there is a marked paucity of wide binary systems near the substellar regime. However, the separation distribution appears to be log-flat, rather than declining as in the field, and the mass ratio distribution is more biased toward similar-mass companions than the initial mass function or the field G-dwarf distribution. The maximum separation also shows no evidence of a limit at ∼ sun . We attribute this result to the post-natal dynamical sculpting that occurs for most field systems; our binary systems will escape to the field intact, but most field stars are formed in denser clusters and undergo significant dynamical evolution. In summary, only wide binary systems with total masses ∼ sun appear to be 'unusually wide'.

Wide Field Infra-Red Survey Telescope (WFIRST) 2.4-Meter Mission Study

Science.gov (United States)

Content, D.; Aaron, K.; Alplanalp, L.; Anderson, K.; Capps, R.; Chang, Z.; Dooley, J.; Egerman, R.; Goullioud, R.; Klein, D.;

Candidate mosaic proteins for a pan-filoviral cytotoxic T-Cell lymphocyte vaccine

Energy Technology Data Exchange (ETDEWEB)

Fenimore, Paul W [Los Alamos National Laboratory; Fischer, William M [Los Alamos National Laboratory; Kuiken, Carla [Los Alamos National Laboratory; Foley, Brian T [Los Alamos National Laboratory; Thurmond, J R [Los Alamos National Laboratory; Yusim, K [Los Alamos National Laboratory; Korber, B T [Los Alamos National Laboratory

2008-01-01

The extremely high fatality rates of many filovirus (FILV) strains the recurrent but rarely identified origin of human epidemics, the only partly identified viral reservoirs and the continuing non-human primate epizootics in Africa make a broadly-protective filovirus vaccine highly desirable. Cytotoxic T-cells (CTL) have been shown to be protective in mice, guinea pigs and non-human primates. In murine models the cytotoxic T-cell epitopes that are protective against Ebola virus have been mapped and in non-human primates CTL-mediated protection between viral strains (John Dye: specify) has been demonstrated using two filoviral proteins, nucleoprotein (NP) and glycoprotein (GP). These immunological results suggest that the CTL avenue of immunity deserves consideration for a vaccine. The poorly-understood viral reservoirs means that it is difficult to predict what strains are likely to cause epidemics. Thus, there is a premium on developing a pan-filoviral vaccine. The genetic diversity of FILV is large, roughly the same scale as human immunodeficiency virus (HIV). This presents a serious challenge for the vaccine designer because a traditional vaccine aspiring to pan-filoviral coverage is likely to require the inclusion of many antigenic reagents. A recent method for optimizing cytotoxic T-cell lymphocyte epitope coverage with mosaic antigens was successful in improving potential CTL epitope coverage against HIV and may be useful in the context of very different viruses, such as the filoviruses discussed here. Mosaic proteins are recombinants composed of fragments of wild-type proteins joined at locations resulting in exclusively natural k-mers, 9 {le} k {le} 15, and having approximately the same length as the wild-type proteins. The use of mosaic antigens is motivated by three conjectures: (1) optimizing a mosaic protein to maximize coverage of k-mers found in a set of reference proteins will give better odds of including broadly-protective CTL epitopes in a vaccine

Stroboscope Based Synchronization of Full Frame CCD Sensors

OpenAIRE

Shen, Liang; Feng, Xiaobing; Zhang, Yuan; Shi, Min; Zhu, Dengming; Wang, Zhaoqi

2017-01-01

The key obstacle to the use of consumer cameras in computer vision and computer graphics applications is the lack of synchronization hardware. We present a stroboscope based synchronization approach for the charge-coupled device (CCD) consumer cameras. The synchronization is realized by first aligning the frames from different video sequences based on the smear dots of the stroboscope, and then matching the sequences using a hidden Markov model. Compared with current synchronized capture equi...

A multiple CCD X-ray detector and its first operation with synchrotron radiation X-ray beam

CERN Document Server

Suzuki, M; Kumasaka, T; Sato, K; Toyokawa, H; Aries, I F; Jerram, P A; Ueki, T

1999-01-01

A 4x4 array structure of 16 identical CCD X-ray detector modules, called the multiple CCD X-ray detector system (MCCDX), was submitted to its first synchrotron radiation experiment at the protein crystallography station of the RIKEN beamline (BL45XU) at the SPring-8 facility. An X-ray diffraction pattern of cholesterol powder was specifically taken in order to investigate the overall system performance.

Construction of a photochemical reactor combining a CCD spectrophotometer and a LED radiation source.

Science.gov (United States)

Gombár, Melinda; Józsa, Éva; Braun, Mihály; Ősz, Katalin

2012-10-01

An inexpensive photoreactor using LED light sources and a fibre-optic CCD spectrophotometer as a detector was built by designing a special cell holder for standard 1.000 cm cuvettes. The use of this device was demonstrated by studying the aqueous photochemical reaction of 2,5-dichloro-1,4-benzoquinone. The developed method combines the highly quantitative data collection of CCD spectrophotometers with the possibility of illuminating the sample independently of the detecting light beam, which is a substantial improvement of the method using diode array spectrophotometers as photoreactors.

Arts and technology - Mosaic new techniques and procedures

Science.gov (United States)

Papiu, G. A.; Suciu, N.

2017-05-01

The relationship between art and technique has been along the time one that is inseparable and systematic, artists appealing to various technologies, tools and practices that help them stimulate their imagination. Today there is a new category of artists, coming from a technical or scientific field, that are being 'trapped’ in this ‘game of art”. The mosaic, even if it is an old technique, responded to the social requirements and it evolved over time, being constantly related to aesthetic and artistic thinking, discoveries of science, assimilating permanent new techniques and technologies, diversifying its artistic forms of expression and methods of transposition. Not being bound any more to a religious institution, which was its birth place, today, she migrated to all public spaces. Works of art in public space have become today an active factor in reshaping the urban aesthetic landscape.

On the involvement of host proteins in Cowpea mosaic virus intercellular spread

NARCIS (Netherlands)

Hollander, den P.W.

2014-01-01

Abstract of thesis Paulus den Hollander entitled “On the involvement of host proteins in Cowpea mosaic virus intercellular spread”.

Defence: 18th of November 13.30 h

Abstract

Intercellular spread of Cowpea mosaic virus (CPMV) occurs via movement

Study of materials and technology of ancient floor mosaics' substrate

Directory of Open Access Journals (Sweden)

Vincenzo Starinieri

2008-01-01

Full Text Available A floor mosaic's substrate is composed of a variety of preparatory layers of mortar built on natural levelled ground or on top of a previous pavement. Mosaics' substrates differ one from the other in number, thickness and nature of the mortar layers. In this sense, it has been considered relevant to state how these differences are related with historical period, geographical position, function of the pavements within the building, technology of the substrates. A number of floor mosaics' substrates of Hellenistic and Roman period from archaeological sites in Greece and Italy are under study. The stratigraphy of substrates is recorded in situ, and samples from each mortar layer are analysed in the laboratory by means of different techniques. Results obtained so far indicate that characteristics of the Roman substrates mortar layers are clearly dependant on their position in the substrate stratigraphy, whereas in the case of the Hellenistic substrates, characteristics of the mortar layers are less varying with the stratigraphic position. Furthermore results show that floor mosaics' substrates are different according to the function of the pavement in the building.

Magnetic properties of mosaic nanocomposites composed of nickel and cobalt nanowires

Energy Technology Data Exchange (ETDEWEB)

Castillo-Sepúlveda, S.; Corona, R.M. [Departamento de Física, Universidad de Santiago de Chile (USACH), Av. Ecuador 3493, 9170124 Santiago (Chile); Altbir, D. [Departamento de Física, Universidad de Santiago de Chile (USACH), Av. Ecuador 3493, 9170124 Santiago (Chile); Center for the Development of Nanoscience and Nanotechnology (CEDENNA), 9170124 Santiago (Chile); Escrig, J., E-mail: juan.escrig@usach.cl [Departamento de Física, Universidad de Santiago de Chile (USACH), Av. Ecuador 3493, 9170124 Santiago (Chile); Center for the Development of Nanoscience and Nanotechnology (CEDENNA), 9170124 Santiago (Chile)

2016-10-15

Mosaic nanocomposites composed of nickel and cobalt nanowires arranged in different configurations were investigated using Monte Carlo simulations and a simple model that considers single-domain structures including length corrections due to the shape anisotropy. Our results showed that for an ordered array both the coercivity and the remanence decrease linearly as a function of the concentration of nickel nanowires. Besides, we obtained that the magnetic properties of an array of a certain hard magnetic material (cobalt) will not change, unless we have more than 50% of nanowires of other soft magnetic material (nickel) in the array. In principle the second material could be other soft magnetic material, but could also be a nonmagnetic material or could even be a situation in which some of the pore arrays were not filled by electrodeposition. Therefore, our results allow us to predict the behavior of magnetic mosaic nanocomposites that are promising candidates for functional electrodes, sensors, and model catalysts. - Highlights: • Mosaic nanocomposites composed of magnetic nanowires were investigated. • Magnetic properties can be adjusted by varying the concentration of nanowires. • Our results allow us to predict the behavior of magnetic mosaic nanocomposites.

An X-ray CCD signal generator with true random arrival time

International Nuclear Information System (INIS)

Huo Jia; Xu Yuming; Chen Yong; Cui Weiwei; Li Wei; Zhang Ziliang; Han Dawei; Wang Yusan; Wang Juan

2011-01-01

An FPGA-based true random signal generator with adjustable amplitude and exponential distribution of time interval is presented. Since traditional true random number generators (TRNG) are resource costly and difficult to transplant, we employed a method of random number generation based on jitter and phase noise in ring oscillators formed by gates in an FPGA. In order to improve the random characteristics, a combination of two different pseudo-random processing circuits is used for post processing. The effects of the design parameters, such as sample frequency are discussed. Statistical tests indicate that the generator can well simulate the timing behavior of random signals with Poisson distribution. The X-ray CCD signal generator will be used in debugging the CCD readout system of the Low Energy X-ray Instrument onboard the Hard X-ray Modulation Telescope (HXMT). (authors)

The Anisotropy of the Microwave Background to l=3500: Mosaic Observations with the Cosmic Background Imager

Science.gov (United States)

Pearson, T. J.; Mason, B. S.; Readhead, A. C. S.; Shepherd, M. C.; Sievers, J. L.; Udomprasert, P. S.; Cartwright, J. K.; Farmer, A. J.; Padin, S.; Myers, S. T.;

A data-acquisition system for high speed linear CCD

International Nuclear Information System (INIS)

Liu Zhiyan; Chen Xiangcai; Jiang Xiaoshan; Zhang Hongyu; Liang Zhongwang; Xiang Haisheng; Hu Jun

2010-01-01

A data-acquisition system for high speed linear CCD (Charge Coupled device) is mainly introduced. The optical fiber transmission technology is used. The data is sent to PC through USB or PCI interface. The construction of the system, the design of the PCI interface hardware, software design and the design of the control program running on host computer are also introduced. (authors)

Geographic mosaic of plant evolution: extrafloral nectary variation mediated by ant and herbivore assemblages.

Directory of Open Access Journals (Sweden)

Anselmo Nogueira

Full Text Available Herbivory is an ecological process that is known to generate different patterns of selection on defensive plant traits across populations. Studies on this topic could greatly benefit from the general framework of the Geographic Mosaic Theory of Coevolution (GMT. Here, we hypothesize that herbivory represents a strong pressure for extrafloral nectary (EFN bearing plants, with differences in herbivore and ant visitor assemblages leading to different evolutionary pressures among localities and ultimately to differences in EFN abundance and function. In this study, we investigate this hypothesis by analyzing 10 populations of Anemopaegma album (30 individuals per population distributed through ca. 600 km of Neotropical savanna and covering most of the geographic range of this plant species. A common garden experiment revealed a phenotypic differentiation in EFN abundance, in which field and experimental plants showed a similar pattern of EFN variation among populations. We also did not find significant correlations between EFN traits and ant abundance, herbivory and plant performance across localities. Instead, a more complex pattern of ant-EFN variation, a geographic mosaic, emerged throughout the geographical range of A. album. We modeled the functional relationship between EFNs and ant traits across ant species and extended this phenotypic interface to characterize local situations of phenotypic matching and mismatching at the population level. Two distinct types of phenotypic matching emerged throughout populations: (1 a population with smaller ants (Crematogaster crinosa matched with low abundance of EFNs; and (2 seven populations with bigger ants (Camponotus species matched with higher EFN abundances. Three matched populations showed the highest plant performance and narrower variance of EFN abundance, representing potential plant evolutionary hotspots. Cases of mismatched and matched populations with the lowest performance were associated

Hubble Space Telescope STIS observations of GRB 000301C: CCD imaging and near-ultraviolet MAMA spectroscopy

DEFF Research Database (Denmark)

Smette, A.; Fruchter, A.S.; Gull, T.R.

2001-01-01

We present Space Telescope Imaging Spectrograph observations of the optical transient (OT) counterpart of the c-ray burster GRB 000301C obtained 5 days after the burst, on 2000 March 6. CCD clear-aperture imaging reveals a R similar or equal to 21.50 +/- 0.15 source with no apparent host galaxy...... Telescope images appear to lie on the stellar field of a host galaxy, and as the large H I column density measured here and in later ground-based observations is unlikely on a random line of sight, we believe we are probably seeing absorption from H I in the host galaxy. In any case, this represents...

Design method of general-purpose driving circuit for CCD based on CPLD

International Nuclear Information System (INIS)

Zhang Yong; Tang Benqi; Xiao Zhigang; Wang Zujun; Huang Shaoyan

2005-01-01

It is very important for studying the radiation damage effects and mechanism systematically about CCD to develop a general-purpose test platform. The paper discusses the design method of general-purpose driving circuit for CCD based on CPLD and the realization approach. A main controller has being designed to read the data file from the outer memory, setup the correlative parameter registers and produce the driving pulses according with parameter request strictly, which is based on MAX7000S by using MAX-PLUS II software. The basic driving circuit module has being finished based on this method. The output waveform of the module is the same figure as the simulation waveform. The result indicates that the design method is feasible. (authors)

Breeding of new variety Yangfumai 4 with high resistance to wheat yellow mosaic disease

International Nuclear Information System (INIS)

He Zhentian; Chen Xiulan; Zhang Rong; Wang Jianhua; Wang Jinrong; Liu Jian

2011-01-01

To control the infection of wheat yellow mosaic disease,new wheat variety with high-yield, disease-resistant was selected. Ningmai 9, which carries yellow mosaic disease resistant genes, was used as original material. Combination of conventional breeding technique and radiation methods, a new wheat variety Yangfumai 4 was developed during 1996-2007, and registered in 2008. The new wheat variety with high yield and resistance to yellow mosaic disease is suitable to plant in the Yangtze River region. (authors)

45,X/47,XXX Mosaicism and Short Stature.

Science.gov (United States)

Everest, Erica; Tsilianidis, Laurie A; Haider, Anzar; Rogers, Douglas G; Raissouni, Nouhad; Schweiger, Bahareh

2015-01-01

We describe the case of a ten-year-old girl with short stature and 45,X/47,XXX genotype. She also suffered from vesicoureteric reflux and kidney dysfunction prior to having surgery on her ureters. Otherwise, she does not have any of the characteristics of Turner nor Triple X syndrome. It has been shown that this mosaic condition as well as other varieties creates a milder phenotype than typical Turner syndrome, which is what we mostly see in our patient. However, this patient is a special case, because she is exceptionally short. Overall, one cannot predict the resultant phenotype in these mosaic conditions. This creates difficulty in counseling parents whose children or fetuses have these karyotypes.

Development of Multiple-Element Flame Emission Spectrometer Using CCD Detection

Science.gov (United States)

Seney, Caryn S.; Sinclair, Karen V.; Bright, Robin M.; Momoh, Paul O.; Bozeman, Amelia D.

2005-01-01

The full wavelength coverage of charge coupled device (CCD) detector when coupled with an echelle spectrography, the system allows for simultaneously multiple element spectroscopy to be performed. The multiple-element flame spectrometer was built and characterized through the analysis of environmentally significant elements such as Ca, K, Na, Cu,…

A generalized measurement equation and van Cittert-Zernike theorem for wide-field radio astronomical interferometry

Science.gov (United States)

Carozzi, T. D.; Woan, G.

2009-05-01

We derive a generalized van Cittert-Zernike (vC-Z) theorem for radio astronomy that is valid for partially polarized sources over an arbitrarily wide field of view (FoV). The classical vC-Z theorem is the theoretical foundation of radio astronomical interferometry, and its application is the basis of interferometric imaging. Existing generalized vC-Z theorems in radio astronomy assume, however, either paraxiality (narrow FoV) or scalar (unpolarized) sources. Our theorem uses neither of these assumptions, which are seldom fulfiled in practice in radio astronomy, and treats the full electromagnetic field. To handle wide, partially polarized fields, we extend the two-dimensional (2D) electric field (Jones vector) formalism of the standard `Measurement Equation' (ME) of radio astronomical interferometry to the full three-dimensional (3D) formalism developed in optical coherence theory. The resulting vC-Z theorem enables full-sky imaging in a single telescope pointing, and imaging based not only on standard dual-polarized interferometers (that measure 2D electric fields) but also electric tripoles and electromagnetic vector-sensor interferometers. We show that the standard 2D ME is easily obtained from our formalism in the case of dual-polarized antenna element interferometers. We also exploit an extended 2D ME to determine that dual-polarized interferometers can have polarimetric aberrations at the edges of a wide FoV. Our vC-Z theorem is particularly relevant to proposed, and recently developed, wide FoV interferometers such as Low Frequency Array (LOFAR) and Square Kilometer Array (SKA), for which direction-dependent effects will be important.

MOSAIC vision and scenarios for mobile collaborative work related to health and wellbeing.

NARCIS (Netherlands)

Jones, Valerie M.; Saranummi, Niilo; Pallot, Marc; Pawar, Kulwant S.

2005-01-01

The main objective of the MOSAIC project is to accelerate innovation in Mobile Worker Support Environments by shaping future research and innovation activities in Europe. The modus operandi of MOSAIC is to develop visions and illustrative scenarios for future collaborative workspaces involving

Somatic Mosaicism Underlies X-linked Acrogigantism (XLAG) Syndrome in Sporadic Male Subjects

Science.gov (United States)

Daly, Adrian F.; Yuan, Bo; Fina, Frederic; Caberg, Jean-Hubert; Trivellin, Giampaolo; Rostomyan, Liliya; de Herder, Wouter W.; Naves, Luciana A.; Metzger, Daniel; Cuny, Thomas; Rabl, Wolfgang; Shah, Nalini; Jaffrain-Rea, Marie-Lise; Zatelli, Maria Chiara; Faucz, Fabio R; Castermans, Emilie; Nanni-Metellus, Isabelle; Lodish, Maya; Muhammad, Ammar; Palmeira, Leonor; Potorac, Iulia; Mantovani, Giovanna; Neggers, Sebastian J.; Klein, Marc; Barlier, Anne; Liu, Pengfei; Ouafik, L'Houcine; Bours, Vincent; Lupski, James R.; Stratakis, Constantine A.; Beckers., Albert

2016-01-01

Somatic mosaicism has been implicated as a causative mechanism in a number of genetic and genomic disorders. X-linked acrogigantism (XLAG) syndrome is a recently characterized genomic form of pediatric gigantism due to aggressive pituitary tumors that is caused by submicroscopic chromosome Xq26.3 duplications that include GPR101. We studied XLAG syndrome patients (N=18) to determine if somatic mosaicism contributed to the genomic pathophysiology. Eighteen subjects with XLAG syndrome were identified with Xq26.3 duplications using high definition array comparative genome hybridization (HD-aCGH). We noted males with XLAG had a decreased log2 ratio compared with expected values, suggesting potential mosaicism, while females showed no such decrease. As compared with familial male XLAG cases, sporadic males had more marked evidence for mosaicism, with levels of Xq26.3 duplication between 16.1-53.8%. These characteristics were replicated using a novel, personalized breakpoint-junction specific quantification droplet digital PCR (ddPCR) technique. Using a separate ddPCR technique we studied the feasibility of identifying XLAG syndrome cases in a distinct patient population of 64 unrelated subjects with acromegaly/gigantism and identified one female gigantism patient that had increased copy number variation (CNV) threshold for GPR101 that was subsequently diagnosed as having XLAG syndrome on HD-aCGH. Employing a combination of HD-aCGH and novel ddPCR approaches, we have demonstrated, for the first time, that XLAG syndrome can be caused by variable degrees of somatic mosaicism for duplications at chromosome Xq26.3. Somatic mosaicism was shown to occur in sporadic males but not in females with XLAG syndrome, although the clinical characteristics of the disease were similarly severe in both sexes. PMID:26935837

Mosaic Stunting in jack pine seedlings in a northern Michigan bareroot nursery

Science.gov (United States)

Lynette Potvin; R. Kasten Dumroese; Martin F. Jurgensen; Dana Richter

2010-01-01

Mosaic, or patchy, stunting of bareroot conifer seedlings is thought to be caused by deficiencies of mycorrhizal fungi following fumigation, resulting in reduced nutrient uptake, particularly phosphorus. Mosaic stunting of jack pine (Pinus banksiana) seedlings was observed in 2005 at the USDA Forest Service JW Toumey Nursery in Watersmeet, MI. We initiated a study to...

Performance of an area variable MOS varicap weighted programmable CCD transversal filter

OpenAIRE

Bhattacharyya, A.B.; Shankarnarayan, L.; Kapur, N.; Wallinga, Hans

1981-01-01

The performance of an electrically programmable CCD transversal filter (PTF) is presented in which tap-weight multiplication is performed by a novel and compact on chip voltage controlled area variable MOS varicap.

Identifikasi Molekuler Tobacco mosaic virus pada Anggrek di Sleman, Yogyakarta

Directory of Open Access Journals (Sweden)

Soesamto Somowiyarjo

2016-05-01

Full Text Available Tobamovirus is a group of virus with a wide host range, including orchid plant which considered as an economically important plant. This research aimed to identify Tobamovirus infecting orchids. Virus isolates were collected from orchid nursery in Sleman, Yogyakarta. Plant extract from orchid showing necrotic flex symptom was inoculated to indicator plants Chenopodium amaranticolor. Chlorotic local lesion symptoms occurred within 3 days after inoculation. RNA total from symptomatic C. amaranticolor was extracted by using a commercial kit. cDNA was synthesized using oligo d(T primer. Amplification of cDNA using partial movement protein specific primers TMV-1F and TMV-2R was successfully amplified the amplicon with size ± 422 bp. The nucleotide sequences of this amplicon  showed highest DNA homology (98% with Tobacco mosaic virus Yongren-2 isolat from China.

Multicultural Mosaic: A Family Book Club.

Science.gov (United States)

Dias-Mitchell, Laurie; Harris, Elizabeth

2001-01-01

Authors, a library media specialist and a literature/language arts teacher, both recipients of Theodore R. Sizer Fellowships, describe their joint project, "Multicultural Mosaic: A Family Book Club." Their proposal was to strengthen the home-school connection by establishing a book club accessible to all middle and high school students…

Proven germline mosaicism in a father of two children with CHARGE syndrome.

Science.gov (United States)

Pauli, S; Pieper, L; Häberle, J; Grzmil, P; Burfeind, P; Steckel, M; Lenz, U; Michelmann, H W

2009-05-01

CHARGE syndrome is an autosomal dominant malformation syndrome caused by mutations in the CHD7 gene. The majority of cases are sporadic and only few familial cases have been reported. In these families, mosaicism in one parent, as well as parent- to-child transmission of a CHD7 mutation, has been described. In some further cases, germline mosaicism has been suggested. Here, we report the first case in which germline mosaicism could be demonstrated in a father of two affected children with CHARGE syndrome. The truncating mutation c.7302dupA in exon 34 of the CHD7 gene was found in both affected children but was not detected in parental lymphocytes. However, in DNA extracted from the father's spermatozoa, the c.7302dupA mutation could be identified. Furthermore, mutation analysis of DNA isolated from 59 single spermatozoa revealed that the c.7302dupA mutation occurs in 16 spermatozoa, confirming germline mosaicism in the father of the affected children. This result has a high impact for genetic counselling of the family and for their recurrence risk in further pregnancies.

Properties of a virus causing mosaic and leaf curl disease of Celosia argentea L. in Nigeria.

Science.gov (United States)

Owolabi, T A; Taiwo, M A; Thottappilly, G A; Shoyinka, S A; Proll, E; Rabenstein, F

1998-06-01

A sap transmissible virus, causing mosaic and leaf curl disease of Celosia argentea, was isolated at vegetable farms in Amuwo Odofin, Tejuoso, and Abule Ado, Lagos, Nigeria. The virus had a restricted host range confined to a few species of the Amaranthaceae, Chenopodiaceae and Solanaceae families. It failed to infect several other species of the Aizoaceae, Brassicaceae, Cucurbitaceae, Fabaceae, Lamiaceae, Malvaceae, Poaceae and Tiliaceae families. The virus was transmitted in a non-persistent manner by Aphis spiraecola and Toxoptera citricidus but not by eight other aphid species tested. There was no evidence of transmission by seeds of C. argentae varieties. The viral coat protein had a relative molecular mass (M(r)) of about 30.2 K. Electron microscopy of purified virus preparations revealed flexuous rod shaped particles of about 750 nm in length. Serological studies were performed using the enzyme-linked immunosorbent assay (ELISA), immunosorbent electron microscopy (ISEM) and Western blot analysis. The virus reacted positively with an universal potyvirus group monoclonal antibody (MoAb) and MoAb P-3-3H8 raised against peanut stripe potyvirus. It also reacted with polyclonal antibodies raised against several potyviruses including asparagus virus-1 (AV-1), turnip mosaic virus (TuMV), maize dwarf mosaic virus (MDMV), watermelon mosaic virus (WMV-2), plum pox virus (PPV), soybean mosaic virus (SoyMV), lettuce mosaic virus (LMV), bean common mosaic virus (BCMV) and beet mosaic virus (BMV) in at least one of the serological assays used. On the basis of host range, mode of transmission, and available literature data, the celosia virus seems to be different from potyviruses previously reported to infect vegetables in Nigeria. The name celosia mosaic virus (CIMV) has been proposed for this virus.

Two Herbig-Haro objects discovered by narrow-band CCD imagery

International Nuclear Information System (INIS)

Ogura, Katsuo

1990-01-01

Two new Herbig-Haro objects, HH 132 and HH 133, have been discovered by CCD imagery behind interference filters on and just off the forbidden S II lines in the red. They are located in Puppis R2 and in Vela R2. Possible locations of their exciting sources are discussed. 12 refs

Wide-field optical mapping of neural activity and brain haemodynamics: considerations and novel approaches

Science.gov (United States)

Ma, Ying; Shaik, Mohammed A.; Kozberg, Mariel G.; Thibodeaux, David N.; Zhao, Hanzhi T.; Yu, Hang

2016-01-01

Although modern techniques such as two-photon microscopy can now provide cellular-level three-dimensional imaging of the intact living brain, the speed and fields of view of these techniques remain limited. Conversely, two-dimensional wide-field optical mapping (WFOM), a simpler technique that uses a camera to observe large areas of the exposed cortex under visible light, can detect changes in both neural activity and haemodynamics at very high speeds. Although WFOM may not provide single-neuron or capillary-level resolution, it is an attractive and accessible approach to imaging large areas of the brain in awake, behaving mammals at speeds fast enough to observe widespread neural firing events, as well as their dynamic coupling to haemodynamics. Although such wide-field optical imaging techniques have a long history, the advent of genetically encoded fluorophores that can report neural activity with high sensitivity, as well as modern technologies such as light emitting diodes and sensitive and high-speed digital cameras have driven renewed interest in WFOM. To facilitate the wider adoption and standardization of WFOM approaches for neuroscience and neurovascular coupling research, we provide here an overview of the basic principles of WFOM, considerations for implementation of wide-field fluorescence imaging of neural activity, spectroscopic analysis and interpretation of results. This article is part of the themed issue ‘Interpreting BOLD: a dialogue between cognitive and cellular neuroscience’. PMID:27574312

Wide-field optical mapping of neural activity and brain haemodynamics: considerations and novel approaches.

Science.gov (United States)

Ma, Ying; Shaik, Mohammed A; Kim, Sharon H; Kozberg, Mariel G; Thibodeaux, David N; Zhao, Hanzhi T; Yu, Hang; Hillman, Elizabeth M C

2016-10-05

Although modern techniques such as two-photon microscopy can now provide cellular-level three-dimensional imaging of the intact living brain, the speed and fields of view of these techniques remain limited. Conversely, two-dimensional wide-field optical mapping (WFOM), a simpler technique that uses a camera to observe large areas of the exposed cortex under visible light, can detect changes in both neural activity and haemodynamics at very high speeds. Although WFOM may not provide single-neuron or capillary-level resolution, it is an attractive and accessible approach to imaging large areas of the brain in awake, behaving mammals at speeds fast enough to observe widespread neural firing events, as well as their dynamic coupling to haemodynamics. Although such wide-field optical imaging techniques have a long history, the advent of genetically encoded fluorophores that can report neural activity with high sensitivity, as well as modern technologies such as light emitting diodes and sensitive and high-speed digital cameras have driven renewed interest in WFOM. To facilitate the wider adoption and standardization of WFOM approaches for neuroscience and neurovascular coupling research, we provide here an overview of the basic principles of WFOM, considerations for implementation of wide-field fluorescence imaging of neural activity, spectroscopic analysis and interpretation of results.This article is part of the themed issue 'Interpreting BOLD: a dialogue between cognitive and cellular neuroscience'. © 2016 The Authors.

Genetics Home Reference: mosaic variegated aneuploidy syndrome

Science.gov (United States)

... In MVA syndrome, growth before birth is slow (intrauterine growth restriction). After birth, affected individuals continue to grow at ... InfoSearch: Warburton Anyane Yeboa syndrome KidsHealth from Nemours: Intrauterine Growth Restriction ... mosaic variegated aneuploidy syndrome 1 MalaCards: ...

An AFLP marker linked to turnip mosaic virus resistance gene in pak ...

African Journals Online (AJOL)

An AFLP marker linked to turnip mosaic virus resistance gene in pak-choi. W Xinhua, C Huoying, Z Yuying, H Ruixian. Abstract. Pak-choi is one of the most important vegetable crops in China. Turnip mosaic virus (TuMV) is one of its main pathogen. Screening the molecular marker linked to the TuMV resistance gene is an ...

CCD polarimetry as a probe of regions of recent-star formation

Energy Technology Data Exchange (ETDEWEB)

Draper, P W

1988-01-01

Chapter 1 of this thesis details the incorporation of a Charged-Coupled Device (CCD) detector system with the Durham Imaging Polarimeter. The details include the physical characteristics of the device and the electronics and software associated with the device control and data storage. Chapter 2 of this work describes fully how suitable corrections for this effect can be made, and derives first-order results. The CCD performance is examined in comparison with the detector used previously and hence the veracity of the new results is established. Chapter 3 is a relevant summary of the status of the astronomy of the immediate regions of recent-star formation. Chapter 4 describes multicolor polarimetry of NGC2261/R Mon covering the period 1979 to 1986. The data conclusively prove that the polarization of R Mon must be due to effects close to R Mon (approx.14 astronomical units).

A simple and accurate method for the quality control of the I.I.-DR apparatus using the CCD camera

International Nuclear Information System (INIS)

Igarashi, Hitoshi; Shiraishi, Akihisa; Kuraishi, Masahiko

2000-01-01

With the advancing development of CCD cameras, the I.I.-DR apparatus has been introduced into the x-ray fluoroscopy television system. Consequently, quality control of the system has become a complicated task. We developed a simple, accurate method for quality control of the I.I.-DR apparatus using the CCD camera. Experiments were separately performed for the imager system [laser imager, DDX (dynamic digital x-ray system)] and the imaging system (I.I., ND-filter, IRIS, CCD camera). Quality control of the imager system was done by simply examining both input and output characteristics with a sliding pattern. Quality control of the imaging system was also conducted by estimating AVE (the average volume element), which was obtained using a phantom under the constant conditions. The results indicated that this simplified method is useful as a weekly quality control check of the I.I.-DR apparatus using the CCD camera. (author)

A CCD-based area detector for X-ray crystallography using synchrotron and laboratory sources

International Nuclear Information System (INIS)

Phillips, W.C.; Li Youli; Stanton, M.; Xie Yuanhui; O'Mara, D.; Kalata, K.

1993-01-01

The design and characteristics of a CCD-based area detector suitable for X-ray crystallographic studies using both synchrotron and laboratory sources are described. The active area is 75 mm in diameter, the FWHM of the point response function is 0.20 mm, and for Bragg peaks the dynamic range is 900 and the DQE ∼0.3. The 1320x1035-pixel Kodak CCD is read out into an 8 Mbyte memory system in 0.14 s and digitized to 12 bits. X-ray crystallographic data collected at the NSLS synchrotron from cubic insulin crystals are presented. (orig.)

Document image mosaicing: A novel approach

Indian Academy of Sciences (India)

R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

MS received 28 April 2003; revised 22 July 2003. Abstract. ... Hence, document image mosaicing is the process of merging split ..... Case 2: Algorithm 2 is an improved version of algorithm 1 which eliminates the drawbacks of ... One of the authors (PS) thanks the All India Council for Technical Education, New Delhi for.

Chromosomal mosaicism in mouse two-cell embryos after paternal exposure to acrylamide

Energy Technology Data Exchange (ETDEWEB)

Marchetti, Francesco; Bishop, Jack; Lowe, Xiu; Wyrobek, Andrew J

2008-10-14

Chromosomal mosaicism in human preimplantation embryos is a common cause ofspontaneous abortions, however, our knowledge of its etiology is limited. We used multicolor fluorescence in situ hybridization (FISH) painting to investigate whether paternally-transmitted chromosomal aberrations result in mosaicism in mouse 2-cell embryos. Paternal exposure to acrylamide, an important industrial chemical also found in tobacco smoke and generated during the cooking process of starchy foods, produced significant increases in chromosomally defective 2-cell embryos, however, the effects were transient primarily affecting the postmeiotic stages of spermatogenesis. Comparisons with our previous study of zygotes demonstrated similar frequencies of chromosomally abnormal zygotes and 2-cell embryos suggesting that there was no apparent selection against numerical or structural chromosomal aberrations. However, the majority of affected 2-cell embryos were mosaics showing different chromosomal abnormalities in the two blastomeric metaphases. Analyses of chromosomal aberrations in zygotes and 2-cell embryos showed a tendency for loss of acentric fragments during the first mitotic division ofembryogenesis, while both dicentrics and translocations apparently underwent propersegregation. These results suggest that embryonic development can proceed up to the end of the second cell cycle of development in the presence of abnormal paternal chromosomes and that even dicentrics can persist through cell division. The high incidence of chromosomally mosaic 2-cell embryos suggests that the first mitotic division of embryogenesis is prone to missegregation errors and that paternally-transmitted chromosomal abnromalities increase the risk of missegregation leading to embryonic mosaicism.

Electrolocation-based underwater obstacle avoidance using wide-field integration methods

International Nuclear Information System (INIS)

Dimble, Kedar D; Faddy, James M; Humbert, J Sean

2014-01-01

Weakly electric fish are capable of efficiently performing obstacle avoidance in dark and navigationally challenging aquatic environments using electrosensory information. This sensory modality enables extraction of relevant proximity information about surrounding obstacles by interpretation of perturbations induced to the fish’s self-generated electric field. In this paper, reflexive obstacle avoidance is demonstrated by extracting relative proximity information using spatial decompositions of the perturbation signal, also called an electric image. Electrostatics equations were formulated for mathematically expressing electric images due to a straight tunnel to the electric field generated with a planar electro-sensor model. These equations were further used to design a wide-field integration based static output feedback controller. The controller was implemented in quasi-static simulations for environments with complicated geometries modelled using finite element methods to demonstrate sense and avoid behaviours. The simulation results were confirmed by performing experiments using a computer operated gantry system in environments lined with either conductive or non-conductive objects acting as global stimuli to the field of the electro-sensor. The proposed approach is computationally inexpensive and readily implementable, making underwater autonomous navigation in real-time feasible. (paper)

Fiber-MZI-based FBG sensor interrogation: comparative study with a CCD spectrometer.

Science.gov (United States)

Das, Bhargab; Chandra, Vikash

2016-10-10

We present an experimental comparative study of the two most commonly used fiber Bragg grating (FBG) sensor interrogation techniques: a charge-coupled device (CCD) spectrometer and a fiber Mach-Zehnder interferometer (F-MZI). Although the interferometric interrogation technique is historically known to offer the highest sensitivity measurements, very little information exists regarding how it compares with the current commercially available spectral-characteristics-based interrogation systems. It is experimentally established here that the performance of a modern-day CCD spectrometer interrogator is very close to a F-MZI interrogator with the capability of measuring Bragg wavelength shifts with sub-picometer-level accuracy. The results presented in this research study can further be used as a guideline for choosing between the two FBG sensor interrogator types for small-amplitude dynamic perturbation measurements down to nano-level strain.

Use of a CCD-based area detection system of a fibre diffractometer

International Nuclear Information System (INIS)

Hanna, S.; Windle, A.H.

1995-01-01

We describe a new X-ray fibre diffractometer, consisting of a commercial X-ray sensitive video camera coupled to a conventional 3 goniometer in place of a more traditional single-point detector. The active element of the video camera is a charge-coupled device (CCD). Diffraction images, obtained at various goniometer settings, are transformed into reciprocal space, and combined to give a complete section through the origin and parallel to the symmetry axis of cylindrically averaged reciprocal space. A greater density of measurements is needed in the vicinity of the reciprocal fibre axis in order to avoid information loss due to the curvature of the Ewald sphere. The pros and cons of using CCD's as X-ray detectors are discussed and sample results from polymer fibers are shown. 17 refs., 5 figs

Polarization leakage in epoch of reionization windows - III. Wide-field effects of narrow-field arrays

Science.gov (United States)

Asad, K. M. B.; Koopmans, L. V. E.; Jelić, V.; de Bruyn, A. G.; Pandey, V. N.; Gehlot, B. K.

2018-05-01

Leakage of polarized Galactic diffuse emission into total intensity can potentially mimic the 21-cm signal coming from the epoch of reionization (EoR), as both of them might have fluctuating spectral structure. Although we are sensitive to the EoR signal only in small fields of view, chromatic side-lobes from further away can contaminate the inner region. Here, we explore the effects of leakage into the `EoR window' of the cylindrically averaged power spectra (PS) within wide fields of view using both observation and simulation of the 3C196 and North Celestial Pole (NCP) fields, two observing fields of the LOFAR-EoR project. We present the polarization PS of two one-night observations of the two fields and find that the NCP field has higher fluctuations along frequency, and consequently exhibits more power at high-k∥ that could potentially leak to Stokes I. Subsequently, we simulate LOFAR observations of Galactic diffuse polarized emission based on a model to assess what fraction of polarized power leaks into Stokes I because of the primary beam. We find that the rms fractional leakage over the instrumental k-space is 0.35 {per cent} in the 3C196 field and 0.27 {per cent} in the NCP field, and it does not change significantly within the diameters of 15°, 9°, and 4°. Based on the observed PS and simulated fractional leakage, we show that a similar level of leakage into Stokes I is expected in the 3C196 and NCP fields, and the leakage can be considered to be a bias in the PS.

Wide field monitoring of the X-ray sky using Rotation Modulation Collimators

DEFF Research Database (Denmark)

Lund, Niels; Brandt, Søren

1995-01-01

Wide field monitoring is of particular interest in X-ray astronomy due to the strong time-variability of most X-ray sources. Not only does the time-profiles of the persistent sources contain characteristic signatures of the underlying physical systems, but, additionally, some of the most intrigui...

CCD Development Progress at Lawrence Berkeley National Laboratory

OpenAIRE

Kolbe, W.F.; Holland, S.E.; Bebek, C.J.

2006-01-01

P-channel CCD imagers, 200-300um thick, fully depleted, and back-illuminat ed are being developed for scientific applications including ground- and space-based astronomy and x-ray detection. These thick devices have extended IR response, good point-spread function (PSF) and excellent radiation tolerance. Initially, these CCDs were made in-house at LBNL using 100 mm diameter wafers. Fabrication on high-resistivity 150 mm wafers is now proceeding according to a model in which the wafers are fir...

Mosaic3: a red-sensitive upgrade for the prime focus camera at the Mayall 4m telescope

Science.gov (United States)

Dey, Arjun; Rabinowitz, David; Karcher, Armin; Bebek, Chris; Baltay, Charles; Sprayberry, David; Valdes, Frank; Stupak, Bob; Donaldson, John; Emmet, Will; Hurteau, Tom; Abareshi, Behzad; Marshall, Bob; Lang, Dustin; Fitzpatrick, Mike; Daly, Phil; Joyce, Dick; Schlegel, David; Schweiker, Heidi; Allen, Lori; Blum, Bob; Levi, Michael

2016-08-01

The Dark Energy Spectroscopic Instrument (DESI) is under construction and will be used to measure the expansion history of the Universe using the Baryon Acoustic Oscillation (BAO) technique and the growth of structure using redshift-space distortions (RSD). The spectra of 30 million galaxies over 14000 sq deg will be measured over the course of the experiment. In order to provide spectroscopic targets for the DESI survey, we are carrying out a three-band (g,r,z ) imaging survey of the sky using the NOAO 4-m telescopes at Kitt Peak National Observatory (KPNO) and the Cerro Tololo Interamerican Observatory (CTIO). At KPNO, we will use an upgraded version of the Mayall 4m telescope prime focus camera, Mosaic3, to carry out a z-band survey of the Northern Galactic Cap at declinations δ>=+30 degrees. By equipping an existing Dewar with four 4kx4k fully depleted CCDs manufactured by the Lawrence Berkeley National Laboratory (LBNL), we increased the z-band throughput of the system by a factor of 1.6. These devices have the thickest active area fielded at a telescope. The Mosaic3 z-band survey will be complemented by g-band and r-band observations using the Bok telescope and 90 Prime imager on Kitt Peak. We describe the upgrade and performance of the Mosaic3 instrument and the scope of the northern survey.

Concurrent insulinoma with mosaic Turner syndrome: A case report.

Science.gov (United States)

Wang, Shaoyun; Yang, Lijuan; Li, Jie; Mu, Yiming

2015-03-01

Turner syndrome is a chromosomal abnormality in which the majority of patients have a 45XO karyotype, while a small number have a 45XO/47XXX karyotype. Congenital adrenal hyperplasia has been previously reported in patients with Turner syndrome. Although insulinomas are the most common type of functioning pancreatic neuroendocrine tumor and have been reported in patients with multiple endocrine neoplasias, the tumors have not been reported in patients with mosaic Turner syndrome. The present study reports the first case of an insulinoma in a patient with 45XO/47XXX mosaic Turner syndrome. The patient suffered from recurrent hypoglycemia, which was relieved following ingestion of glucose or food. A 5-h glucose tolerance test was performed and the levels of glucose, C-Peptide and insulin were detected. In addition, computed tomography (CT) and ultrasound scanning were performed to evaluate the possibility of an insulinoma. Pathological examination and karyotyping were performed on a surgical specimen and a whole blood sample, respectively. The patient was found to suffer from premature ovarian failure, and a physical examination was consistent with a diagnosis of Turner syndrome. An ultrasound scan demonstrated streak ovaries and the patient was found to have a 45XO/47XXX karyotype. Furthermore, a lesion was detected in the pancreas following CT scanning, which was identified as an insulinoma following surgical removal and histological examination. In conclusion, the present study reports the first case of an insulinoma in a patient with mosaic Turner syndrome. Since mosaic Turner syndrome and insulinoma are rare diseases, an association may exist that has not been previously identified.

Design and Applications of Rapid Image Tile Producing Software Based on Mosaic Dataset

Science.gov (United States)

Zha, Z.; Huang, W.; Wang, C.; Tang, D.; Zhu, L.

2018-04-01

Map tile technology is widely used in web geographic information services. How to efficiently produce map tiles is key technology for rapid service of images on web. In this paper, a rapid producing software for image tile data based on mosaic dataset is designed, meanwhile, the flow of tile producing is given. Key technologies such as cluster processing, map representation, tile checking, tile conversion and compression in memory are discussed. Accomplished by software development and tested by actual image data, the results show that this software has a high degree of automation, would be able to effectively reducing the number of IO and improve the tile producing efficiency. Moreover, the manual operations would be reduced significantly.

DESIGN AND APPLICATIONS OF RAPID IMAGE TILE PRODUCING SOFTWARE BASED ON MOSAIC DATASET

Directory of Open Access Journals (Sweden)

Z. Zha

2018-04-01

Full Text Available Map tile technology is widely used in web geographic information services. How to efficiently produce map tiles is key technology for rapid service of images on web. In this paper, a rapid producing software for image tile data based on mosaic dataset is designed, meanwhile, the flow of tile producing is given. Key technologies such as cluster processing, map representation, tile checking, tile conversion and compression in memory are discussed. Accomplished by software development and tested by actual image data, the results show that this software has a high degree of automation, would be able to effectively reducing the number of IO and improve the tile producing efficiency. Moreover, the manual operations would be reduced significantly.

First-light instrument for the 3.6-m Devasthal Optical Telescope: 4Kx4K CCD Imager

Science.gov (United States)

Pandey, Shashi Bhushan; Yadav, Rama Kant Singh; Nanjappa, Nandish; Yadav, Shobhit; Reddy, Bheemireddy Krishna; Sahu, Sanjit; Srinivasan, Ramaiyengar

2018-04-01

As a part of in-house instrument developmental activity at ARIES, the 4Kx4K CCD Imager is designed and developed as a first-light instrument for the axial port of the 3.6-m Devasthal Optical Telescope (DOT). The f/9 beam of the telescope having a plate-scale of 6.4"/mm is utilized to conduct deeper photom-etry within the central 10' field of view. The pixel size of the blue-enhanced liquid nitrogen cooled STA4150 4Kx4K CCD chip is 15 μm, with options to select gain and speed values to utilize the dynamic range. Using the Imager, it is planned to image the central 6.5'x6.5' field of view of the telescope for various science goals by getting deeper images in several broad-band filters for point sources and objects with low surface brightness. The fully assembled Imager along with automated filter wheels having Bessel UBV RI and SDSS ugriz filters was tested in late 2015 at the axial port of the 3.6-m DOT. This instrument was finally mounted at the axial port of the 3.6-m DOT on 30 March 2016 when the telescope was technically activated jointly by the Prime Ministers of India and Belgium. It is expected to serve as a general purpose multi-band deep imaging instrument for a variety of science goals including studies of cosmic transients, active galaxies, star clusters and optical monitoring of X-ray sources discovered by the newly launched Indian space-mission called ASTROSAT, and follow-up of radio bright objects discovered by the Giant Meterwave Radio Telescope.

Caracterização espectral de áreas de gramíneas forrageiras infectadas com a doença "mela-das-sementes da braquiária" por meio de imagens CCD/CBERS-2 Spectral characterization of forage grasses infected with the disease "mela-das-sementes da braquiária" through CCD/CDBERS -2 images

Directory of Open Access Journals (Sweden)

José C. Rosatti

2006-12-01

Full Text Available Imagens CCD/CBERS-2, nas bandas espectrais CCD2, CCD3 e CCD4, dos anos de 2004 e 2005, de Mirante do Paranapanema - SP, foram transformadas em reflectância de superfície usando o modelo 5S de correção atmosférica e normalizadas radiometricamente. O objetivo principal foi caracterizar espectralmente áreas de pastagens de Brachiaria brizantha em fase de florescimento, isentas e infectadas com a doença "mela-das-sementes da braquiária", possibilitando a sua detecção por meio da comparação entre os valores de reflectância de superfície denominada de Fator de Reflectância Bidirecional de Superfície (FRBS. Teve-se, também, o objetivo de avaliar a eficácia das imagens CCD/CBERS-2 para a obtenção de respostas espectrais de pastagens. Os dosséis sadios e doentes da Brachiaria brizantha foram identificados por meio da análise dos valores de reflectância e dos dados observados no Índice de Estresse Hídrico Acumulativo Relativo da Cultura (ACWSI obtidos na área de estudo. Os resultados indicaram que as principais diferenças foram a diminuição da reflectância na banda CCD3 e o aumento da reflectância na banda CCD4 nas áreas doentes. A metodologia empregada com o uso de dados do sensor CCD/CBERS-2, associados ao ACWSI, mostrou-se eficaz para discriminar dosséis infectados com a "mela-das-sementes da braquiária".CCD/CBERS-2 images in the spectral bands of CCD2, CCD3 and CCD4 of the years 2004 and 2005, from Mirante do Paranapanema - SP (Brazil, were transformed into surface reflectance images using the 5S atmospheric correction model and radiometrically normalized. The main objective was to spectrally characterize pastures of Brachiaria brizantha in the flowering phase, exempt and infected with the disease "mela-das-sementes da braquiária" making it possible its detection through the comparison among the SBRF - Surface Bidirectional Reflectance Factor values. At the same time, it was aimed to evaluate the effectiveness of the

FNTD radiation dosimetry system enhanced with dual-color wide-field imaging

International Nuclear Information System (INIS)

Akselrod, M.S.; Fomenko, V.V.; Bartz, J.A.; Ding, F.

2014-01-01

At high neutron and photon doses Fluorescent Nuclear Track Detectors (FNTDs) require operation in analog mode and the measurement results depend on individual crystal color center concentration (coloration). We describe a new method for radiation dosimetry using FNTDs, which includes non-destructive, automatic sensitivity calibration for each individual FNTD. In the method presented, confocal laser scanning fluorescent imaging of FNTDs is combined with dual-color wide field imaging of the FNTD. The calibration is achieved by measuring the color center concentration in the detector through fluorescence imaging and reducing the effect of diffuse reflection on the lapped surface of the FNTD by imaging with infra-red (IR) light. The dual-color imaging of FNTDs is shown to provide a good estimation of the detector sensitivity at high doses of photons and neutrons, where conventional track counting is impeded by track overlap. - Highlights: • New method and optical imaging head was developed for FNTD used at high doses. • Dual-color wide-field imaging used for color center concentration measurement. • Green fluorescence corrected by diffuse reflection used for sensitivity correction. • FNTD dose measurements performed in analog processing mode

MOSAIC: An organic geochemical and sedimentological database for marine surface sediments

Science.gov (United States)

Tavagna, Maria Luisa; Usman, Muhammed; De Avelar, Silvania; Eglinton, Timothy

2015-04-01

Modern ocean sediments serve as the interface between the biosphere and the geosphere, play a key role in biogeochemical cycles and provide a window on how contemporary processes are written into the sedimentary record. Research over past decades has resulted in a wealth of information on the content and composition of organic matter in marine sediments, with ever-more sophisticated techniques continuing to yield information of greater detail and as an accelerating pace. However, there has been no attempt to synthesize this wealth of information. We are establishing a new database that incorporates information relevant to local, regional and global-scale assessment of the content, source and fate of organic materials accumulating in contemporary marine sediments. In the MOSAIC (Modern Ocean Sediment Archive and Inventory of Carbon) database, particular emphasis is placed on molecular and isotopic information, coupled with relevant contextual information (e.g., sedimentological properties) relevant to elucidating factors that influence the efficiency and nature of organic matter burial. The main features of MOSAIC include: (i) Emphasis on continental margin sediments as major loci of carbon burial, and as the interface between terrestrial and oceanic realms; (ii) Bulk to molecular-level organic geochemical properties and parameters, including concentration and isotopic compositions; (iii) Inclusion of extensive contextual data regarding the depositional setting, in particular with respect to sedimentological and redox characteristics. The ultimate goal is to create an open-access instrument, available on the web, to be utilized for research and education by the international community who can both contribute to, and interrogate the database. The submission will be accomplished by means of a pre-configured table available on the MOSAIC webpage. The information on the filled tables will be checked and eventually imported, via the Structural Query Language (SQL), into

CCD photometry of Cepheid sequences in four nearby galaxies

International Nuclear Information System (INIS)

Metcalfe, N.; Shanks, T.

1991-01-01

We present Isaac Newton Telescope B and V CCD observations of deep photometric sequences in the vicinity of Cepheid variable stars in three nearby galaxies - M31, M33 and NGC 2403. We have also checked the photometry of the brightest stars in M81 and its dwarf companion, Holmberg IX. We use our data, combined with other recent results, to re-analyse the Cepheid distances to these galaxies. (author)

Wide-field kinematic structure of early-type galaxy halos

Science.gov (United States)

Arnold, Jacob Antony

2013-12-01

The stellar halos of nearby galaxies bare the signatures of the mass-assembly processes that have driven galaxy evolution over the last ˜10 Gyr. Finding and interpreting these relict clues in galaxies within and beyond the local group offers one of the most promising avenues for understanding how galaxies accumulate their stars over time. To tackle this problem we have performed a systematic study of the wide-field kinematic structure of nearby (Dspectroscopy out to several effective radii (˜3 R e). The 22 galaxies presented here span a range of environments (field, group, and cluster), intrinsic luminosities (-22.4 infrared Calcium II triplet. For each spectrum, we parameterize the line-of-sight velocity distribution (LOSVD) as a truncated Gauss-Hermite series convolved with an optimally weighted combination of stellar templates. These kinematic measurements (V, sigma, h3, and h4) are combined with literature values to construct spatially resolved maps of large-scale kinematic structure. A variety of kinematic behaviors are observed beyond ~1 Re, potentially reflecting the stochastic and chaotic assembly of stellar bulges and halos in early-type galaxies. Next, we describe a global analysis (out to 5 Re) of kinematics and metallicity in the nearest S0 galaxy, NGC 3115, along with implications for its assembly history. The data include high-quality wide-field imaging and multi-slit spectra of the field stars and globular clusters (GCs). Within two effective radii, the bulge (as traced by the stars and metal-rich GCs) is flattened and rotates rapidly. At larger radii, the rotation declines dramatically, while the characteristic GC metallicities also decrease with radius. We argue that this pattern is not naturally explained by a binary major merger, but instead by a two-phase assembly process where the inner regions have formed in an early violent, dissipative phase, followed by the protracted growth of the outer parts via minor mergers. To test this hypothesis

Suche nach solaren Axionen mit dem CCD-Detektor in CAST (CERN Axion Solar Telescope)

CERN Document Server

Kang, Donghwa

2007-01-01

The CERN Axion Solar Telescope (CAST) experiment at CERN searches for solar axions with energies in the keV range. Axions could be produced in the Sun's core by the interaction of thermal photons with virtual photons of the strong electromagnetic field. In this experiment, the solar axions can be converted to photons in the field of a 9 Tesla superconducting magnet. At both ends of the 10 m long dipole magnet, three different X-ray detectors were installed, which are sensitive in the interesting photon energy range. This thesis is devoted to the determination of an upper limit on the axion-photon coupling constant g$_{a\\gamma}$. The analysis is based on the data taken by the CCD detector in the CAST experiment during the years 2003 and 2004. First results of the 2003 data taking were published showing no significant signal above background. However, these results constrain the upper limit of the axion-photon coupling constant by a factor 5 compared to previous axion search experiments. Moreover, the result of...

Measuring neutron fluences and gamma/x-ray fluxes with CCD cameras

International Nuclear Information System (INIS)

Yates, G.J.; Smith, G.W.; Zagarino, P.; Thomas, M.C.

1991-01-01

The capability to measure bursts of neutron fluences and gamma/x-ray fluxes directly with charge coupled device (CCD) cameras while being able to distinguish between the video signals produced by these two types of radiation, even when they occur simultaneously, has been demonstrated. Volume and area measurements of transient radiation-induced pixel charge in English Electric Valve (EEV) Frame Transfer (FT) charge coupled devices (CCDs) from irradiation with pulsed neutrons (14 MeV) and Bremsstrahlung photons (4--12 MeV endpoint) are utilized to calibrate the devices as radiometric imaging sensors capable of distinguishing between the two types of ionizing radiation. Measurements indicate ∼.05 V/rad responsivity with ≥1 rad required for saturation from photon irradiation. Neutron-generated localized charge centers or ''peaks'' binned by area and amplitude as functions of fluence in the 10 5 to 10 7 n/cm 2 range indicate smearing over ∼1 to 10% of CCD array with charge per pixel ranging between noise and saturation levels

Important considerations for radiochromic film dosimetry with flatbed CCD scanners and EBT GAFCHROMIC[reg] film

International Nuclear Information System (INIS)

Lynch, Bart D.; Kozelka, Jakub; Ranade, Manisha K.; Li, Jonathan G.; Simon, William E.; Dempsey, James F.

2006-01-01

In this study, we present three significant artifacts that have the potential to negatively impact the accuracy and precision of film dosimetry measurements made using GAFCHROMIC[reg] EBT radiochromic film when read out with CCD flatbed scanners. Films were scanned using three commonly employed instruments: a Macbeth TD932 spot densitometer, an Epson Expression 1680 CCD array scanner, and a Microtek ScanMaker i900 CCD array scanner. For the two scanners we assessed the variation in optical density (OD) of GAFCHROMIC EBT film with scanning bed position, angular rotation of the film with respect to the scan line direction, and temperature inside the scanner due to repeated scanning. Scanning uniform radiochromic films demonstrated a distinct bowing effect in profiles in the direction of the CCD array with a nonuniformity of up to 17%. Profiles along a direction orthogonal to the CCD array demonstrated a 7% variation. A strong angular dependence was found in measurements made with the flatbed scanners; the effect could not be reproduced with the spot densitometer. An IMRT quality assurance film was scanned twice rotating the film 90 deg. between the scans. For films scanned on the Epson scanner, up to 12% variation was observed in unirradiated EBT films rotated between 0 deg. and 90 deg. , which decreased to approximately 8% for EBT films irradiated to 300 cGy. Variations of up to 80% were observed for films scanned with the Microtek scanner. The scanners were found to significantly increase the film temperature with repeated scanning. Film temperature between 18 and 33 deg. C caused OD changes of approximately 7%. Considering these effects, we recommend adherence to a strict scanning protocol that includes: maintaining the orientation of films scanned on flatbed scanners, limiting scanning to the central portion of the scanner bed, and limiting the number of consecutive scans to minimize changes in OD caused by film heating

Identification of Prader-Willi Syndrome mosaicism by fluorescent in situ hybridization

Energy Technology Data Exchange (ETDEWEB)

Mowery-Rushton, P.A.; Surti, U. [Magee Womens Hospital, Pittsburgh, PA (United States); Hanchett, J.M. [Rehabilitation Institute, Pittsburgh, PA (United States)

1994-09-01

Prader-Willi Syndrome (PWS) is a microdeletion syndrome involving an interstitial deletion of region q11-q13 on the paternal chromosome 15. We report 2 cases of PWS that were analyzed using FISH and were found to be mosaic for a normal cell line and a deleted cell line. Case 1 was diagnosed as an atypical PWS who was cytogenetically normal. She is a 38 y.o. white female displaying some but not all of the features of PWS. Case 2 is a 23 y.o. white male with a classical deletion of chromosome 15q11-q13. He displays very typical features of PWS. He was also noted to be albino. FISH analysis was performed on PHA stimulated lymphocytes. We examined four loci: D15S11, SNRPN, D15S10, and GABRB3. The number of cells examined for each locus ranged from 46 to 75. Case 1 was deleted at 3 of the 4 loci (D15S11, SNRPN and GABRB3) in 30% of her cells. The D15S10 locus was not deleted. This may account for the atypical features displayed by this patient. It also suggests that this chromosome is rearranged resulting in the retention of the interstitial locus. The exact nature of the rearrangement needs to be determined. Case 2 was deleted at all four loci in 60% of the cells analyzed. This result was unexpected because his deletion was identified cytogenetically, but mosaicism was not detected. These are the first reported cases of mosaic PWS diagnosed using FISH. The use of cytogenetics alone requires high resolution banding to accurately identify the deletion. This makes the detection of small deletions in every cell difficult and the determination of mosaicism almost impossible. Our results suggest that mosaicism may be occurring more frequently than previously thought and may account for some of the atypical cases. Studies are in progress to determine the effect of mosaicism on methylation at genes located within this region which are imprinted and are thought to be involved in the etiology of Prader-Willi Syndroms.

1935 15' Quad #292 Aerial Photo Mosaic Index

Data.gov (United States)

Earth Data Analysis Center, University of New Mexico — Aerial Photo Reference Mosaics contain aerial photographs that are retrievable on a frame by frame basis. The inventory contains imagery from various sources that...

1935 15' Quad #246 Aerial Photo Mosaic Index

Data.gov (United States)

Earth Data Analysis Center, University of New Mexico — Aerial Photo Reference Mosaics contain aerial photographs that are retrievable on a frame by frame basis. The inventory contains imagery from various sources that...

1935 15' Quad #364 Aerial Photo Mosaic Index

Data.gov (United States)

Earth Data Analysis Center, University of New Mexico — Aerial Photo Reference Mosaics contain aerial photographs that are retrievable on a frame by frame basis. The inventory contains imagery from various sources that...

1935 15' Quad #056 Aerial Photo Mosaic Index

Data.gov (United States)

Earth Data Analysis Center, University of New Mexico — Aerial Photo Reference Mosaics contain aerial photographs that are retrievable on a frame by frame basis. The inventory contains imagery from various sources that...

1935 15' Quad #032 Aerial Photo Mosaic Index

Data.gov (United States)

Earth Data Analysis Center, University of New Mexico — Aerial Photo Reference Mosaics contain aerial photographs that are retrievable on a frame by frame basis. The inventory contains imagery from various sources that...

1935 15' Quad #063 Aerial Photo Mosaic Index

Data.gov (United States)

Earth Data Analysis Center, University of New Mexico — Aerial Photo Reference Mosaics contain aerial photographs that are retrievable on a frame by frame basis. The inventory contains imagery from various sources that...

1935 15' Quad #147 Aerial Photo Mosaic Index