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Sample records for washington col wassell

  1. Mutation analysis of COL4A3 and COL4A4 genes in a Chinese

    Indian Academy of Sciences (India)

    Autosomal dominant Alport syndrome (ADAS) accounts for 5% of all cases of Alport syndrome (AS), a primary basement membrane disorder arising from mutations in genes encoding the type IV collagen protein family.Mutationsin COL4A3 and COL4A4 genes were reported to be associated with ADAS. In this study, clinical ...

  2. Mutation analysis of COL4A3 and COL4A4 genes in a Chinese ...

    Indian Academy of Sciences (India)

    2016-10-24

    ,. People's Republic of China ... mutations in COL4A3 and COL4A4 genes which encode type IV collagen α3 and α4 chainsrespectively can .... hematuria and evidence for activation of the unfolded protein response. Focal and ...

  3. Detection of COL III in Parchment by Amino Acid Analysis

    DEFF Research Database (Denmark)

    Vestergaard Poulsen Sommer, Dorte; Larsen, René

    2016-01-01

    Cultural heritage parchments made from the reticular dermis of animals have been subject to studies of deterioration and conservation by amino acid analysis. The reticular dermis contains a varying mixture of collagen I and III (COL I and III). When dealing with the results of the amino acid...... analyses, till now the COL III content has not been taken into account. Based on the available amino acid sequences we present a method for determining the amount of COL III in the reticular dermis of new and historical parchments calculated from the ratio of Ile/Val. We find COL III contents between 7...... and 32 % in new parchments and between 0.2 and 40 % in the historical parchments. This is consistent with results in the literature. The varying content of COL III has a significant influence on the uncertainty of the amino acid analysis. Although we have not found a simple correlation between the COL...

  4. Washington Conference

    International Nuclear Information System (INIS)

    Anon.

    1981-01-01

    The 1981 Particle Accelerator Conference was held in Washington from 11-13 March. It was the ninth in the series of meetings organized in the USA which differ from the 'International' meetings in their coverage of the full range of accelerator engineering and technology, including applications outside e field of high energy physics. The Conference took place under the cloud of further budget cuts for Fiscal Year 1982 in the USA which the Department of Energy has applied in line with the financial policy of the new administration. Coming on top of many years of budget trimming which have reduced the number of high energy physics Laboratories funded by the DOE to three (Brookhaven, Fermilab, Stanford - Cornell is funded by the National Science Foundation) and reduced the exploitation of these Laboratories to less than half of their potential, the new cuts did not exactly help to boost morale. Nevertheless, the huge amount of tailed work in accelerator physics and technology which was presented at the Conference showed how alive the field is

  5. Childhood presentation of COL4A1 mutations

    NARCIS (Netherlands)

    Shah, S.; Ellard, S.; Kneen, R.; Lim, M.; Osborne, N.; Rankin, J.; Stoodley, N.; van der Knaap, M.S.; Whitney, A.; Jardine, P.

    2012-01-01

    Aim To describe the clinical and radiological features of four new families with a childhood presentation of COL4A1 mutation. Method We retrospectively reviewed the clinical presentation. Investigations included radiological findings and COL4A1 mutation analysis of the four cases. Affected family

  6. Childhood presentation of COL4A1 mutations

    NARCIS (Netherlands)

    Shah, Siddharth; Ellard, Sian; Kneen, Rachel; Lim, Ming; Osborne, Nigel; Rankin, Julia; Stoodley, Neil; van der Knaap, Marjo; Whitney, Andrea; Jardine, Philip

    2012-01-01

    To describe the clinical and radiological features of four new families with a childhood presentation of COL4A1 mutation. We retrospectively reviewed the clinical presentation. Investigations included radiological findings and COL4A1 mutation analysis of the four cases. Affected family members were

  7. Mutational analysis of COL1A1 and COL1A2 genes among Estonian osteogenesis imperfecta patients.

    Science.gov (United States)

    Zhytnik, Lidiia; Maasalu, Katre; Reimann, Ene; Prans, Ele; Kõks, Sulev; Märtson, Aare

    2017-08-15

    Osteogenesis imperfecta (OI) is a rare bone disorder. In 90% of cases, OI is caused by mutations in the COL1A1/2 genes, which code procollagen α1 and α2 chains. The main aim of the current research was to identify the mutational spectrum of COL1A1/2 genes in Estonian patients. The small population size of Estonia provides a unique chance to explore the collagen I mutational profile of 100% of OI families in the country. We performed mutational analysis of peripheral blood gDNA of 30 unrelated Estonian OI patients using Sanger sequencing of COL1A1 and COL1A2 genes, including all intron-exon junctions and 5'UTR and 3'UTR regions, to identify causative OI mutations. We identified COL1A1/2 mutations in 86.67% of patients (26/30). 76.92% of discovered mutations were located in the COL1A1 (n = 20) and 23.08% in the COL1A2 (n = 6) gene. Half of the COL1A1/2 mutations appeared to be novel. The percentage of quantitative COL1A1/2 mutations was 69.23%. Glycine substitution with serine was the most prevalent among missense mutations. All qualitative mutations were situated in the chain domain of pro-α1/2 chains. Our study shows that among the Estonian OI population, the range of collagen I mutations is quite high, which agrees with other described OI cohorts of Northern Europe. The Estonian OI cohort differs due to the high number of quantitative variants and simple missense variants, which are mostly Gly to Ser substitutions and do not extend the chain domain of COL1A1/2 products.

  8. Mutation analysis of the COL1A1 and COL1A2 genes in Vietnamese patients with osteogenesis imperfecta.

    Science.gov (United States)

    Ho Duy, Binh; Zhytnik, Lidiia; Maasalu, Katre; Kändla, Ivo; Prans, Ele; Reimann, Ene; Märtson, Aare; Kõks, Sulev

    2016-08-12

    The genetics of osteogenesis imperfecta (OI) have not been studied in a Vietnamese population before. We performed mutational analysis of the COL1A1 and COL1A2 genes in 91 unrelated OI patients of Vietnamese origin. We then systematically characterized the mutation profiles of these two genes which are most commonly related to OI. Genomic DNA was extracted from EDTA-preserved blood according to standard high-salt extraction methods. Sequence analysis and pathogenic variant identification was performed with Mutation Surveyor DNA variant analysis software. Prediction of the pathogenicity of mutations was conducted using Alamut Visual software. The presence of variants was checked against Dalgleish's osteogenesis imperfecta mutation database. The sample consisted of 91 unrelated osteogenesis imperfecta patients. We identified 54 patients with COL1A1/2 pathogenic variants; 33 with COL1A1 and 21 with COL1A2. Two patients had multiple pathogenic variants. Seventeen novel COL1A1 and 10 novel COL1A2 variants were identified. The majority of identified COL1A1/2 pathogenic variants occurred in a glycine substitution (36/56, 64.3 %), usually serine (23/36, 63.9 %). We found two pathogenic variants of the COL1A1 gene c.2461G > A (p.Gly821Ser) in four unrelated patients and one, c.2005G > A (p.Ala669Thr), in two unrelated patients. Our data showed a lower number of collagen OI pathogenic variants in Vietnamese patients compared to reported rates for Asian populations. The OI mutational profile of the Vietnamese population is unique and related to the presence of a high number of recessive mutations in non-collagenous OI genes. Further analysis of OI patients negative for collagen mutations, is required.

  9. Evaluation the COL9A2 gene with high myopia

    Science.gov (United States)

    Zhang, Dingding; Huang, Maomin

    2017-11-01

    This paper investigates the association of the COL9A2 gene between high myopia and normal controls in the Han Chinese population. It shows that the frameshift mutation (D281fs) in the COL9A2 gene is not associated with high myopia in the Han Chinese population, and the two novel variants(c.143G>C and c.884G>A) may contribute to the development of high myopia.

  10. University of Washington

    Data.gov (United States)

    Federal Laboratory Consortium — The theme of the University of Washington based Center for Child Environmental Health Risks Research (CHC) is understanding the biochemical, molecular and exposure...

  11. Tuberculose du col utérin simulant un cancer du col utérin : à propos ...

    African Journals Online (AJOL)

    Tuberculose du col utérin simulant un cancer du col utérin : à propos d'un cas, au Centre Hospitalier Universitaire Souro Sanou de Bobo-Dioulasso, Burkina Faso. A. Dembélé, V. Konségré, E. Birba, D.A. Somé, H. Zamané, A.S. Ouédraogo, S. Kiemtoré, S. Ouattara, A. Lamien-Sanou, M. Bambara, B. Bonané/Thiéba ...

  12. EuroCirCol: A key to New Physics

    CERN Multimedia

    Johannes Gutleber

    2015-01-01

    Monday 1 June saw the start of EuroCirCol, the EC-funded part of the FCC study that will develop the conceptual design for an energy-frontier hadron collider.   Attendees at the EuroCirCol meeting at CERN. The EuroCirCol kick-off event at CERN on 2 to 4 June brought together 62 participants to constitute governance bodies, commit to the project plan and align the organisation, structures and processes of 16 institutions from 10 countries. The goal of the project is to conceive a post-LHC research infrastructure around a 100 km circular energy-frontier hadron collider capable of reaching 100 TeV collisions. The project officially started on 1 June and will run for four years. The total estimated budget of 11.2 MEUR includes a 2.99 MEUR contribution from the Horizon 2020 programme dedicated to the development of new world-class research infrastructures. EuroCirCol will deliver a design for a hadron collider as part of the broader Future Circular Collider (FCC) study. It will provide input to an...

  13. LT COL F.F. PIENAAR'S BOER WAR DIARY

    African Journals Online (AJOL)

    LT COL F.F. PIENAAR'S BOER WAR DIARY. (PART II). Brig J.H. Picard, SM*. FREE STATE TO ..... bing up and down on his master's spare horse. ... Jantjie and his master turned out of the road, and ..... on a list which burghers could afford to.

  14. Complete COL1A1 allele deletions in osteogenesis imperfecta

    NARCIS (Netherlands)

    van Dijk, Fleur S.; Huizer, Margriet; Kariminejad, Ariana; Marcelis, Carlo L.; Plomp, Astrid S.; Terhal, Paulien A.; Meijers-Heijboer, Hanne; Weiss, Marjan M.; van Rijn, Rick R.; Cobben, Jan M.; Pals, Gerard

    Purpose: To identify a molecular genetic cause in patients with a clinical diagnosis of osteogenesis imperfecta (OI) type I/IV. Methods: The authors performed multiplex ligation-dependent probe amplification analysis of the COL1A1 gene in a group of 106 index patients. Results: In four families with

  15. Complete COL1A1 allele deletions in osteogenesis imperfecta

    NARCIS (Netherlands)

    van Dijk, Fleur S.; Huizer, Margriet; Kariminejad, Ariana; Marcelis, Carlo L.; Plomp, Astrid S.; Terhal, Paulien A.; Meijers-Heijboer, Hanne; Weiss, Marjan M.; van Rijn, Rick R.; Cobben, Jan M.; Pals, Gerard

    2010-01-01

    To identify a molecular genetic cause in patients with a clinical diagnosis of osteogenesis imperfecta (OI) type I/IV. The authors performed multiplex ligation-dependent probe amplification analysis of the COL1A1 gene in a group of 106 index patients. In four families with mild osteogenesis

  16. Dietary Lecithin Decreases Skeletal Muscle COL1A1 and COL3A1 Gene Expression in Finisher Gilts

    Directory of Open Access Journals (Sweden)

    Henny Akit

    2016-06-01

    Full Text Available The purpose of this study was to investigate the effect of dietary lecithin on skeletal muscle gene expression of collagen precursors and enzymes involved in collagen synthesis and degradation. Finisher gilts with an average start weight of 55.9 ± 2.22 kg were fed diets containing either 0, 4, 20 or 80 g/kg soybean lecithin prior to harvest for six weeks and the rectus abdominis muscle gene expression profile was analyzed by quantitative real-time PCR. Lecithin treatment down-regulated Type I (α1 procollagen (COL1A1 and Type III (α1 procollagen (COL3A1 mRNA expression ( p < 0.05, respectively, indicating a decrease in the precursors for collagen synthesis. The α-subunit of prolyl 4-hydroxylase (P4H mRNA expression also tended to be down-regulated ( p = 0.056, indicating a decrease in collagen synthesis. Decreased matrix metalloproteinase-1 (MMP-1 mRNA expression may reflect a positive regulatory response to the reduced collagen synthesis in muscle from the pigs fed lecithin ( p = 0.035. Lecithin had no effect on tissue inhibitor metalloproteinase-1 (TIMP-1, matrix metalloproteinase-13 (MMP-13 and lysyl oxidase mRNA expression. In conclusion, lecithin down-regulated COL1A1 and COL3A1 as well as tended to down-regulate α-subunit P4H expression. However, determination of muscle collagen content and solubility are required to support the gene functions.

  17. Critical early roles for col27a1a and col27a1b in zebrafish notochord morphogenesis, vertebral mineralization and post-embryonic axial growth.

    Science.gov (United States)

    Christiansen, Helena E; Lang, Michael R; Pace, James M; Parichy, David M

    2009-12-29

    Fibrillar collagens are well known for their links to human diseases, with which all have been associated except for the two most recently identified fibrillar collagens, type XXIV collagen and type XXVII collagen. To assess functions and potential disease phenotypes of type XXVII collagen, we examined its roles in zebrafish embryonic and post-embryonic development. We identified two type XXVII collagen genes in zebrafish, col27a1a and col27a1b. Both col27a1a and col27a1b were expressed in notochord and cartilage in the embryo and early larva. To determine sites of type XXVII collagen function, col27a1a and col27a1b were knocked down using morpholino antisense oligonucleotides. Knockdown of col27a1a singly or in conjunction with col27a1b resulted in curvature of the notochord at early stages and formation of scoliotic curves as well as dysmorphic vertebrae at later stages. These defects were accompanied by abnormal distributions of cells and protein localization in the notochord, as visualized by transmission electron microscopy, as well as delayed vertebral mineralization as detected histologically. Together, our findings indicate a key role for type XXVII collagen in notochord morphogenesis and axial skeletogenesis and suggest a possible human disease phenotype.

  18. Mutation Analysis of COL1A1 and COL1A2 in Fetuses with Osteogenesis Imperfecta Type II/III.

    Science.gov (United States)

    Wang, Wenbo; Wu, Qichang; Cao, Lin; Sun, Li; Xu, Yasong; Guo, Qiwei

    2015-01-27

    Aim: To analyze COL1A1/2 mutations in prenatal-onset OI for determine the proportion of mutations in type I collagen genes among prenatal onset OI and to provide additional data for genotype-phenotype analyses. Material and Methods: Ten cases of severe fetal short-limb dwarfism detected by antenatal ultrasonography were referred to our center. Before the termination of pregnancy, cordocentesis was performed for fetal karyotype and COL1A1/2 gene sequencing analysis. Postmortem radiographic examination was performed at all instances for definitive diagnosis. Results: COL1A1 and COL1A2 SNP and mutations were identified in all the cases. Among these, one synonymous SNP and four synonymous SNPs were recognized in COL1A1/2, respectively, seven cases have distinct heterozygous mutations and six new COL1A1/2 gene mutations were identified. Conclusion: There has been substantial progress in the identification of the molecular defects responsible for skeletal dysplasias. With the constant increase in the number of identified mutations in COL1A1 and COL1A2, genotype-phenotype correlation is becoming increasingly pertinent. © 2015 S. Karger AG, Basel.

  19. The transmembrane collagen COL-99 guides longitudinally extending axons in C. elegans.

    Science.gov (United States)

    Taylor, Jesse; Unsoeld, Thomas; Hutter, Harald

    2018-06-01

    We have identified the transmembrane collagen, COL-99, in a genetic screen for novel genes involved in axon guidance in the nematode C. elegans. COL-99 is similar to transmembrane collagens type XIII, XXIII and XXV in vertebrates. col-99 mutants exhibit guidance defects in axons extending along the major longitudinal axon tracts, most prominently the left ventral nerve cord (VNC). COL-99 is expressed in the hypodermis during the time of axon outgrowth. We provide evidence that a furin cleavage site in COL-99 is essential for function, suggesting that COL-99 is released from the cells producing it. Vertebrate homologs of COL-99 have been shown to be expressed in mammalian nervous systems and linked to various neurological disease but have not been associated with guidance of extending neurons. col-99 acts genetically with the discoidin domain receptors ddr-1 and ddr-2, which are expressed by neurons affected in col-99 mutants. Discoidin domain receptors are activated by collagens in vertebrates. DDR-1 and DDR-2 may function as receptors for COL-99. Our results establish a novel role for a transmembrane collagen in axonal guidance and asymmetry establishment of the VNC. Copyright © 2018 Elsevier Inc. All rights reserved.

  20. Col4a1 mutations cause progressive retinal neovascular defects and retinopathy.

    Science.gov (United States)

    Alavi, Marcel V; Mao, Mao; Pawlikowski, Bradley T; Kvezereli, Manana; Duncan, Jacque L; Libby, Richard T; John, Simon W M; Gould, Douglas B

    2016-01-27

    Mutations in collagen, type IV, alpha 1 (COL4A1), a major component of basement membranes, cause multisystem disorders in humans and mice. In the eye, these include anterior segment dysgenesis, optic nerve hypoplasia and retinal vascular tortuosity. Here we investigate the retinal pathology in mice carrying dominant-negative Col4a1 mutations. To this end, we examined retinas longitudinally in vivo using fluorescein angiography, funduscopy and optical coherence tomography. We assessed retinal function by electroretinography and studied the retinal ultrastructural pathology. Retinal examinations revealed serous chorioretinopathy, retinal hemorrhages, fibrosis or signs of pathogenic angiogenesis with chorioretinal anastomosis in up to approximately 90% of Col4a1 mutant eyes depending on age and the specific mutation. To identify the cell-type responsible for pathogenesis we generated a conditional Col4a1 mutation and determined that primary vascular defects underlie Col4a1-associated retinopathy. We also found focal activation of Müller cells and increased expression of pro-angiogenic factors in retinas from Col4a1(+/Δex41)mice. Together, our findings suggest that patients with COL4A1 and COL4A2 mutations may be at elevated risk of retinal hemorrhages and that retinal examinations may be useful for identifying patients with COL4A1 and COL4A2 mutations who are also at elevated risk of hemorrhagic strokes.

  1. The transcription factor Lc-Maf participates in Col27a1 regulation during chondrocyte maturation

    Energy Technology Data Exchange (ETDEWEB)

    Mayo, Jaime L.; Holden, Devin N. [Department of Microbiology and Molecular Biology, Brigham Young University, 591 WIDB, Provo, UT 84602 (United States); Barrow, Jeffery R. [Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT 84602 (United States); Bridgewater, Laura C., E-mail: laura_bridgewater@byu.edu [Department of Microbiology and Molecular Biology, Brigham Young University, 591 WIDB, Provo, UT 84602 (United States)

    2009-08-01

    The transcription factor Lc-Maf, which is a splice variant of c-Maf, is expressed in cartilage undergoing endochondral ossification and participates in the regulation of type II collagen through a cartilage-specific Col2a1 enhancer element. Type XXVII and type XI collagens are also expressed in cartilage during endochondral ossification, and so enhancer/reporter assays were used to determine whether Lc-Maf could regulate cartilage-specific enhancers from the Col27a1 and Col11a2 genes. The Col27a1 enhancer was upregulated over 4-fold by Lc-Maf, while the Col11a2 enhancer was downregulated slightly. To confirm the results of these reporter assays, rat chondrosarcoma (RCS) cells were transiently transfected with an Lc-Maf expression plasmid, and quantitative RT-PCR was performed to measure the expression of endogenous Col27a1 and Col11a2 genes. Endogenous Col27a1 was upregulated 6-fold by Lc-Maf overexpression, while endogenous Col11a2 was unchanged. Finally, in situ hybridization and immunohistochemistry were performed in the radius and ulna of embryonic day 17 mouse forelimbs undergoing endochondral ossification. Results demonstrated that Lc-Maf and Col27a1 mRNAs are coexpressed in proliferating and prehypertrophic regions, as would be predicted if Lc-Maf regulates Col27a1 expression. Type XXVII collagen protein was also most abundant in prehypertrophic and proliferating chondrocytes. Others have shown that mice that are null for Lc-Maf and c-Maf have expanded hypertrophic regions with reduced ossification and delayed vascularization. Separate studies have indicated that Col27a1 may serve as a scaffold for ossification and vascularization. The work presented here suggests that Lc-Maf may affect the process of endochondral ossification by participating in the regulation of Col27a1 expression.

  2. Colágeno na cartilagem osteoartrótica

    Directory of Open Access Journals (Sweden)

    Ana Paula P. Velosa

    2003-06-01

    Full Text Available A cartilagem articular é um tecido altamente especializado, composto por células, os condrócitos, e um conjunto de macromoléculas, como o colágeno e os proteoglicanos. O colágeno é uma proteína fibrilar que garante resistência ao tecido, enquanto os proteoglicanos têm a função de mola biológica, sendo responsáveis pela compressibilidade da cartilagem. A complexa interação entre estas duas proteínas garante a elasticidade. Estas características específicas da cartilagem são essenciais para amortecer as grandes forças de impacto a que as articulações diartrodiais estão submetidas, sem muito gasto de energia, visto tratar-se de um tecido avascular. Em processos artrósicos ocorre um desequilíbrio entre a produção de componentes da matriz extracelular e destruição pelas metaloproteases, levando à degradação e perda do tecido cartilaginoso. A fase inicial da osteoartrose é marcada por perda de fragmentos de proteoglicanos para o líquido sinovial, aumento dos colágenos tipo II e tipo VI, aparecimento dos colágenos I e III, não típicos da cartilagem, e diminuição do colágeno tipo IX, que é importante para manter a integridade da matriz extracelular, além do entumescimento da cartilagem. Como conseqüência, a cartilagem perde suas características específicas, levando a alterações na função articular. A evolução da doença promove diminuição significativa das proteínas, até mesmo do colágeno tipo XI, que tem localização mais interna na estrutura da fibrila heterotípica, e, portanto levando até a exposição do osso. Até o momento, o tratamento da osteoartrose está baseado principalmente no controle da dor e/ou inflamação, não diminuindo ou impedindo a degradação da cartilagem articular. Neste aspecto a perspectiva de tratamento futuro da osteoartrose estaria na utilização de inibidores das metaloproteases associadas a condroprotetores interferindo no "turnover" da cartilagem e

  3. Recent developments: Washington focus

    International Nuclear Information System (INIS)

    Anon.

    1990-01-01

    November was a quiet month in Washington. Although Congress has recessed until 1991, the Senate filled vacancies in party leadership positions created by November's elections. The House is expected to proceed with its changes in early December. The Nuclear Energy Forum was held in Washington, DC on November 11-14 to discuss the status of the nuclear industry in the USA. The Forum, held in conjunction with the American Nuclear Society's annual meeting, assembled a large number of CEO's from US, European, and Far Eastern utilities and vendors. The meeting concluded with an announcement by Philip Bayne, President of NYPA and chairman of the Nuclear Power Oversight Committee (NPOC), of the results of a year-long NPOC study entitled a open-quotes Strategic Plan for Building New Nuclear Power Plants.close quotes

  4. Upper bound on the non-colorability threshold of the 2+p-COL problem

    International Nuclear Information System (INIS)

    Cruz, R.

    2004-10-01

    The 2p-COL problem introduced by Walsh, smoothly interpolates between P and NP by mixing together the polynornial 2-coloring problem and the NP complete 3-coloring problem. A natural upper bound on the non colorability of the 2+p-COL problem in min {r-bar 2 /(1 - p)r-bar 3 }, where r-bar 2 and r-bar 3 are the upper bounds on 2-COL and 3-COL thresholds respectively. In this paper we improve this upper-bound for each 0.73 ≤ p 1. This means that for p ≥ 0.73 the 2+p-COL problem does not behave like the 2-COL problem. We use the method developed by Kaporis et al., which combines the concept of legal rigid colorings introduced by Achlioptas and Molloy with the occupancy problem for random allocations of balls into bins. (author)

  5. EuroCirCol - Horizon 2020 Research and Innovation Action

    CERN Document Server

    AUTHOR|(CDS)2108815; Benedikt, Michael; Zimmermann, Frank; Schulte, Daniel; Tommasini, Davide; Seryi, Andrei; Perez, Francis

    2015-01-01

    EuroCirCol is a conceptual design study for a post-LHC research infrastructure based on an energy-frontier 100 TeV circular hadron collider. ​The award of the 2013 Nobel Prize for Physics acknowledged the leading role of Europe in particle physics, which has a global community of over 10'000 scientists. To reinforce its pole position throughout the 21st century, Europe must be ready to propose an ambitious post-LHC accelerator project by 2018/19. This is one of the main recommendations of the updated European Strategy for Particle Physics, adopted by the CERN Council in May 2013. The EuroCirCol conceptual design study is a direct response to this recommendation, carried out through a collaboration of institutes and universities worldwide. A new research infrastructure of such scale depends on the feasibility of key technologies pushed beyond current state of the art. Innovative designs for accelerator magnets to achieve high-quality fields up to 16 T and for a cryogenic beam vacuum system to cope with unpre...

  6. Cisto gigante de colédoco Giant choledochal cyst

    Directory of Open Access Journals (Sweden)

    Olival Cirilo Lucena da Fonseca-Neto

    2007-12-01

    Full Text Available INTRODUÇÃO: A doença cística das vias biliares é anomalia congênita que pode acometer as vias biliares intra e/ou extra-hepáticas. A extra-hepática inclui os cistos de colédoco e a intra-hepática é conhecida por Doença de Caroli. Os cistos de colédoco de tamanho gigante são muito raros. OBJETIVO: Relatar o caso de um cisto de colédoco de tamanho gigante em uma paciente feminina. RELATO DE CASO: Mulher de 19 anos foi admitida com história de icterícia e acolia fecal há sete dias. Referia dor epigástrica associada com ingestão de dieta rica em gordura. Nos antecedentes pessoais relatou dois episódios de icterícia, aos 8 e 14 anos, que progrediram espontaneamente. No exame físico apresentava icterícia (+3/+4 e uma massa palpável indolor em mesogástrio foram os únicos achados. A ultrassonografia demonstrou grande formação cística de paredes finas adjacente ao hepatocolédoco, pâncreas e rim direito que media 18,5 x 10,2 cm. A colangioressonância confirmou o grande cisto de colédoco e hepatojejunoanastomose em "Y" de Roux após excisão do cisto e colecistectomia foi realizada. A formação cística media 20 x 15,5 x 12,5 cm e com um volume médio de 1000 mL. A paciente encontra-se em acompanhamento ambulatorial sem alterações hepatobiliares após o sétimo mês da operação. CONCLUSÃO: O cisto de colédoco deve fazer parte do diagnóstico diferencial em pacientes adultos jovens com icterícia e massa palpável; no entanto, a diferenciação entre ele e neoplasia maligna deve ser pesquisada.BACKGROUND: Choledochal cyst represents a rare congenital anomaly, eventually associated with intra and extrahepatic biliary tract disorders. Extrahepatic diseases include choledochal cysts and congenital dilation of the lower intrahepatic bile duct is known as Caroli's disease. Giant choledochal cyst constitutes a very rare abnormality. AIM:To report a giant choledochal cyst in a female patient. CASE REPORT: A 19-year

  7. EuroCirCol kick-off event

    CERN Multimedia

    Hardre, Julie

    2015-01-01

    The EuroCirCol (http://cern.ch/eurocircol) kick-off event at CERN on June 2-4 brought together 62 participants to constitute governance bodies, commit to the project plan and align the organisation, structures and processes of 16 institutions from 10 countries. The goal of the project is to conceive a post-LHC research infrastructure around a 100 km circular energy-frontier hadron collider capable of reaching 100 TeV collisions. The project officially started on June 1 and will run for four years. The total estimated budget of 11.2 million Euros includes a 2.99 million Euro contribution from the Horizon 2020 programme on developing new world-class research infrastructures (http://cordis.europa.eu/project/rcn/194962_en.html).

  8. Entomopathogenic fungi in predatory beetles (Col: Carabidae and Staphylinidae) from agricultural fields

    DEFF Research Database (Denmark)

    Steenberg, T; Langer, V; Esbjerg, P

    1995-01-01

    Prevalence of entomopathogenic fungi was studied in overwintering ground beetles (Col.: Carabidae) and rove beetles (Col.: Staphylinidae) collected from fields of lucerne, white cabbage and white cabbage undersown with white clover. In general infection levels in adult ground beetles and rove bee...

  9. Washington State Biofuels Industry Development

    Energy Technology Data Exchange (ETDEWEB)

    Gustafson, Richard [Univ. of Washington, Seattle, WA (United States)

    2017-04-09

    The funding from this research grant enabled us to design, renovate, and equip laboratories to support University of Washington biofuels research program. The research that is being done with the equipment from this grant will facilitate the establishment of a biofuels industry in the Pacific Northwest and enable the University of Washington to launch a substantial biofuels and bio-based product research program.

  10. Fetal origin of brain damage in 2 infants with a COL4A1 mutation: fetal and neonatal MRI

    NARCIS (Netherlands)

    Vermeulen, R. J.; Peeters-Scholte, C.; van Vugt, J. J. M.; van Vught, J. J. M. G.; Barkhof, F.; Rizzu, P.; van der Schoor, S. R. D.; van der Knaap, M. S.

    2011-01-01

    Mutations in the gene COL4A1, encoding collagen IV A1, are associated with familial porencephaly. Previously, COL4A1 mutation-associated antenatal hemorrhages have been suggested by early post-natal imaging. We describe 2 children with fetal intracerebral hemorrhages and a COL4A1 mutation. There was

  11. Congress in Washington

    International Nuclear Information System (INIS)

    Anon.

    1993-01-01

    Full text: Over 1200 accelerator physicists and engineers gathered in Washington mid-May for the 15th in the series of biennial Particle Accelerator Conferences (PAC) - the major US forum for accelerator physics and technology. For the first time since their inception, actual attendance declined, however the number of contributed papers stayed around 1500. CERN Director General designate Chris Llewellyn Smith spelled out the challenges with an opening talk underlining the deficiencies in today's Standard Model. From many directions comes the message that collision in the 1 TeV range must tell us something new - wherefore art thou SSC and LHC? The secondary shock waves of last year's (fortunately overturned) bid to cancel the SSC Superconducting Supercollider project still ripple around the USA, while progress towards authorization of CERN's LHC Large Hadron Collider has been slower than initially hoped. The new US administration has indicated a constant rate of SSC funding over the next four years; the figure is higher than the present budget but considerably below the originally proposed budget profile, implying that completion will be retarded by some three years beyond the end of the decade. The SSC Laboratory will clearly have problems to fight increased overall cost and sustain enthusiasm. CERN hopes for LHC blessing in time to allow machine completion by the year 2000. Pride of place at Washington went to DESY's HERA electron-proton collider - the major new facility since the previous PAC. Commissioning has been impressive and physics is well underway, with luminosity climbing towards the design figure. The varied user community of the ubiquitous synchrotron radiation facilities is now considerably larger than that of particle physics and has extensive industrial involvement. Three such machines have come into operation since the previous PAC - the 6 GeV European Synchrotron Radiation Facility at Grenoble, the 1.5 GeV Advanced Light Source

  12. Collimation and material science studies (ColMat) at GSI.

    CERN Document Server

    Stadlmann, J; Kollmus, H; Krause, M; Mustafin, E; Petzenhauser, I; Spiller, P; Strasik, I; Tahir, N; Tomut, M; Trautmann, C

    2010-01-01

    Within the frame of the EuCARD program, the GSI Helmholtzzentrum für Schwerionenforschung in Darmstadt is performing accelerator R&D in workpackage 8: ColMat. The coordinated effort is focussed on materials aspects important for building the FAIR accelerator facility at GSI and the LHC upgrade at CERN. Accelerator components and especially protection devices have to be operated in high dose environments. The radiation hazard occurs either by the primary proton and ion beams or the secondary radiation after initial beam loss. Detailed numerical simulations have been carried out to study the damage caused to solid targets by the full impact of the LHC beam as well as the SPS beam. Tungsten, copper and graphite as possible collimator materials have been studied. Experimental an theoretical studies on radiation damage on materials used for the LHC upgrade and the FAIR accelerators are performed at the present GSI experimental facilities. Technical decisions based on these results will have an impact on the F...

  13. Washington Accelerator Conference

    International Nuclear Information System (INIS)

    Anon.

    1993-01-01

    Highlights of the 1993 Particle Accelerator Conference, held in Washington in May, were picked out in the previous issue (page 18). Talks on the big hadron colliders reflected the sea-change in the accelerator world where the scale, complexity and cost of the front-line projects has slowed the pace of developments (not unlike the scene in particle physics itself). Speaking before the anti-SSC vote in the House of Representatives in June, Dick Briggs reviewed the situation at the SSC Superconducting Supercollider in Ellis County, Texas. The linac building is near completion and the Low Energy Booster will be ready to receive components early next year. Tunnelling for the Main Ring is advancing rapidly with four boring machines in action. Five miles of tunnel have been completed since January and the pace has now stepped up to nearly a mile each week. The superconducting magnet news is good. Following the successful initial string test of a half cell of the magnet lattice, a two-ring full cell with all associated services is being assembled. The mechanical robustness of the magnet design was confirmed when a dipole was taken to 9.7 T when cooled to 1.8 K. In the Magnet Test Lab itself, ten test stands are installed and equipped

  14. Recent developments: Washington focus

    International Nuclear Information System (INIS)

    Anon.

    1990-01-01

    Congress reconvened on January 23, but most of Washington's January new involves the Administration. DOE sent two letters to USEC customers, awarded a contract for the independent financial review of the enrichment program, and released a plan for demonstrating AVLIS by 1992. A General Accounting Office (GAO) report investigating the impact of imports of Soviet EUP into the US was made public. Both Congress and the administration are reportedly considering a full-scope US-Soviet Agreement for Nuclear Cooperation. Finally, published reports indicate Congress may consider ending the customs user fee which levies a charge of 0.17% on the value of all imported goods. The fee is felt to violate the General Agreement on Tariffs and Trade (GATT) and is not based on recovering actual Customs costs for processing a good. The fee brings the Treasury over $700 million per year, but the business community plans to lobby hard for its outright elimination or a change in authority to collect the fee based on actual costs

  15. Washington Accelerator Conference

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1993-09-15

    Highlights of the 1993 Particle Accelerator Conference, held in Washington in May, were picked out in the previous issue (page 18). Talks on the big hadron colliders reflected the sea-change in the accelerator world where the scale, complexity and cost of the front-line projects has slowed the pace of developments (not unlike the scene in particle physics itself). Speaking before the anti-SSC vote in the House of Representatives in June, Dick Briggs reviewed the situation at the SSC Superconducting Supercollider in Ellis County, Texas. The linac building is near completion and the Low Energy Booster will be ready to receive components early next year. Tunnelling for the Main Ring is advancing rapidly with four boring machines in action. Five miles of tunnel have been completed since January and the pace has now stepped up to nearly a mile each week. The superconducting magnet news is good. Following the successful initial string test of a half cell of the magnet lattice, a two-ring full cell with all associated services is being assembled. The mechanical robustness of the magnet design was confirmed when a dipole was taken to 9.7 T when cooled to 1.8 K. In the Magnet Test Lab itself, ten test stands are installed and equipped.

  16. The ColM Family, Polymorphic Toxins Breaching the Bacterial Cell Wall

    Directory of Open Access Journals (Sweden)

    Maarten G. K. Ghequire

    2018-02-01

    Full Text Available Bacteria host an arsenal of antagonism-mediating molecules to combat for ecologic space. Bacteriocins represent a pivotal group of secreted antibacterial peptides and proteins assisting in this fight, mainly eliminating relatives. Colicin M, a model for peptidoglycan-interfering bacteriocins in Gram-negative bacteria, appears to be part of a set of polymorphic toxins equipped with such a catalytic domain (ColM targeting lipid II. Diversifying recombination has enabled parasitism of different receptors and has also given rise to hybrid bacteriocins in which ColM is associated with another toxin module. Remarkably, ColM toxins have recruited a diverse array of immunity partners, comprising cytoplasmic membrane-associated proteins with different topologies. Together, these findings suggest that different immunity mechanisms have evolved for ColM, in contrast to bacteriocins with nuclease activities.

  17. Genetics Home Reference: COL4A1-related brain small-vessel disease

    Science.gov (United States)

    ... hemorrhage Johns Hopkins Medicine Department of Neurology and Neurosurgery: Intracerebral Hemorrhage Johns Hopkins Medicine Department of Neurology and Neurosurgery: Stroke MalaCards: col4a1-related brain small-vessel disease ...

  18. 12 CFR 4.4 - Washington office.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Washington office. 4.4 Section 4.4 Banks and... EXAMINERS Organization and Functions § 4.4 Washington office. The Washington office of the OCC is the main office and headquarters of the OCC. The Washington office directs OCC policy, oversees OCC operations...

  19. Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis

    International Nuclear Information System (INIS)

    Kibe, A.; Shimada, K.; Tagaki, Y.

    1979-01-01

    Hybrid ColE1 plasmids called ColE1-cos lambda-guaA or ColE1-cos lambda-gal can be efficiently transduced into various E.coli K-12 cells through packaging into lambda phage particles. Using these plasmids, repair of ultraviolet-light (UV) damaged ColE1 DNAs was studied in various UV sensitive E.coli K-12 mutants. The host mutations uvrA and uvrB markedly reduced host-cell reactivation of UV-irradiated ColE1-cos lambda-guaA. Pre-existing hybrid ColE1 plasmids had no effect on the frequency of lambda phage-mediated transduction of another differentially marked hybrid ColE1 DNAs. ColE1-cos lambda-guaA and ColE1-cos lambda-gal DNAs could temporarily but not stably co-exist in E.coli K-12 recA cells. The presence of ColE1-cos lambda-gal in uvrB cells promoted the repair of super-infected UV-irradiated ColE1-cos lambda-guaA about 7-fold. The same ColE1-cos lambda-gal plasmid in a uvrB recA double mutant did not have this promoting effect. These results indicate that the effect of resident hybrid ColE1 plasmids is manifested by the host recA + gene function(s) and suggest that ColE1 plasmit itself provides no recA + -like functions. (orig.) [de

  20. Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis

    Energy Technology Data Exchange (ETDEWEB)

    Kibe, A; Shimada, K; Tagaki, Y [Kyushu Univ., Fukuoka (Japan). Dept. of Biochemistry

    1979-01-01

    Hybrid ColE1 plasmids called ColE1-cos lambda-guaA or ColE1-cos lambda-gal can be efficiently transduced into various E.coli K-12 cells through packaging into lambda phage particles. Using these plasmids, repair of ultraviolet-light (UV) damaged ColE1 DNAs was studied in various UV sensitive E.coli K-12 mutants. The host mutations uvrA and uvrB markedly reduced host-cell reactivation of UV-irradiated ColE1-cos lambda-guaA. Pre-existing hybrid ColE1 plasmids had no effect on the frequency of lambda phage-mediated transduction of another differentially marked hybrid ColE1 DNAs. ColE1-cos lambda-guaA and ColE1-cos lambda-gal DNAs could temporarily but not stably co-exist in E.coli K-12 recA cells. The presence of ColE1-cos lambda-gal in uvrB cells promoted the repair of super-infected UV-irradiated ColE1-cos lambda-guaA about 7-fold. The same ColE1-cos lambda-gal plasmid in a uvrB recA double mutant did not have this promoting effect. These results indicate that the effect of resident hybrid ColE1 plasmids is manifested by the host recA/sup +/ gene function(s) and suggest that ColE1 plasmit itself provides no recA/sup +/-like functions.

  1. Persistent Notochord in a Fetus with COL2A1 Mutation

    Directory of Open Access Journals (Sweden)

    Elisabeth Codsi

    2015-01-01

    Full Text Available Multiple anomalies including micromelia, poor mineralization of the vertebrae, and a persistent notochord were identified on second trimester ultrasound in a fetus with a COL2A1 mutation. To our knowledge, this represents the first case of a persistent notochord associated with a COL2A1 mutation in humans. In this case report, we describe ultrasound and postmortem findings and review the pathogenesis associated with a persistent notochord.

  2. COL11A1 in FAP polyps and in sporadic colorectal tumors

    International Nuclear Information System (INIS)

    Fischer, Heléne; Salahshor, Sima; Stenling, Roger; Björk, Jan; Lindmark, Gudrun; Iselius, Lennart; Rubio, Carlos; Lindblom, Annika

    2001-01-01

    We previously reported that the α-1 chain of type 11 collagen (COL11A1), not normally expressed in the colon, was up-regulated in stromal fibroblasts in most sporadic colorectal carcinomas. Patients with germline mutations in the APC gene show, besides colonic polyposis, symptoms of stromal fibroblast involvement, which could be related to COL11A1 expression. Most colorectal carcinomas are suggested to be a result of an activated Wnt- pathway, most often involving an inactivation of the APC gene or activation of β-catenin. We used normal and polyp tissue samples from one FAP patient and a set of 37 sporadic colorectal carcinomas to find out if the up-regulation of COL11A1 was associated with an active APC/β-catenin pathway. In this study we found a statistically significant difference in COL11A1 expression between normal tissue and adenomas from one FAP patient, and all adenomas gave evidence for an active APC/β-catenin pathway. An active Wnt pathway has been suggested to involve stromal expression of WISP-1. We found a strong correlation between WISP-1 and COL11A1 expression in sporadic carcinomas. Our results suggest that expression of COL11A1 in colorectal tumors could be associated with the APC/β-catenin pathway in FAP and sporadic colorectal cancer

  3. Historical changes to Lake Washington and route of the Lake Washington Ship Canal, King County, Washington

    Science.gov (United States)

    Chrzastowski, Michael J.

    1983-01-01

    Lake Washington, in the midst of the greater Seattle metropolitan area of the Puget Sound region (fig. 1), is an exceptional commercial, recreational, and esthetic resource for the region . In the past 130 years, Lake Washington has been changed from a " wild " lake in a wilderness setting to a regulated lake surrounded by a growing metropolis--a transformation that provides an unusual opportunity to study changes to a lake's shoreline and hydrologic characteristics -resulting from urbanization.

  4. Mutations in COL1A1 and COL1A2 and dental aberrations in children and adolescents with osteogenesis imperfecta - A retrospective cohort study.

    Directory of Open Access Journals (Sweden)

    Kristofer Andersson

    Full Text Available Osteogenesis imperfecta (OI is a heterogeneous group of disorders of connective tissue, caused mainly by mutations in the collagen I genes (COL1A1 and COL1A2. Dentinogenesis imperfecta (DGI and other dental aberrations are common features of OI. We investigated the association between collagen I mutations and DGI, taurodontism, and retention of permanent second molars in a retrospective cohort of 152 unrelated children and adolescents with OI. The clinical examination included radiographic evaluations. Teeth from 81 individuals were available for histopathological evaluation. COL1A1/2 mutations were found in 104 individuals by nucleotide sequencing. DGI was diagnosed clinically and radiographically in 29% of the individuals (44/152 and through isolated histological findings in another 19% (29/152. In the individuals with a COL1A1 mutation, 70% (7/10 of those with a glycine substitution located C-terminal of p.Gly305 exhibited DGI in both dentitions while no individual (0/7 with a mutation N-terminal of this point exhibited DGI in either dentition (p = 0.01. In the individuals with a COL1A2 mutation, 80% (8/10 of those with a glycine substitution located C terminal of p.Gly211 exhibited DGI in both dentitions while no individual (0/5 with a mutation N-terminal of this point (p = 0.007 exhibited DGI in either dentition. DGI was restricted to the deciduous dentition in 20 individuals. Seventeen had missense mutations where glycine to serine was the most prevalent substitution (53%. Taurodontism occurred in 18% and retention of permanent second molars in 31% of the adolescents. Dental aberrations are strongly associated with qualitatively changed collagen I. The varying expressivity of DGI is related to the location of the collagen I mutation. Genotype information may be helpful in identifying individuals with OI who have an increased risk of dental aberrations.

  5. Scoliosis in osteogenesis imperfecta caused by COL1A1/COL1A2 mutations - genotype-phenotype correlations and effect of bisphosphonate treatment.

    Science.gov (United States)

    Sato, Atsuko; Ouellet, Jean; Muneta, Takeshi; Glorieux, Francis H; Rauch, Frank

    2016-05-01

    Bisphosphonates are widely used to treat children with osteogenesis imperfecta (OI), a bone fragility disorder that is most often caused by mutations in COL1A1 or COL1A2. However, it is unclear whether this treatment decreases the risk of developing scoliosis. We retrospectively evaluated spine radiographs and charts of 437 patients (227 female) with OI caused by mutations in COL1A1 or COL1A2 and compared the relationship between scoliosis, genotype and bisphosphonate treatment history. At the last follow-up (mean age 11.9 [SD: 5.9] years), 242 (55%) patients had scoliosis. The prevalence of scoliosis was highest in OI type III (89%), followed by OI type IV (61%) and OI type I (36%). Moderate to severe scoliosis (Cobb angle ≥25°) was rare in individuals with COL1A1 haploinsufficiency mutations but was present in about two fifth of patients with triple helical glycine substitutions or C-propeptide mutations. During the first 2 to 4years of bisphosphonate therapy, patients with OI type III had lower Cobb angle progression rates than before bisphosphonate treatment, whereas in OI types I and IV bisphosphonate treatment was not associated with a change in Cobb angle progression rates. At skeletal maturity, the prevalence of scoliosis (Cobb angle >10°) was similar in patients who had started bisphosphonate treatment early in life (before 5.0years of age) and in patients who had started therapy later (after the age of 10.0years) or had never received bisphosphonate therapy. Bisphosphonate treatment decreased progression rate of scoliosis in OI type III but there was no evidence of a positive effect on scoliosis in OI types I and IV. The prevalence of scoliosis at maturity was not influenced by the bisphosphonate treatment history in any OI type. Copyright © 2016 Elsevier Inc. All rights reserved.

  6. Mutations in COL1A1 and COL1A2 and dental aberrations in children and adolescents with osteogenesis imperfecta – A retrospective cohort study

    Science.gov (United States)

    Dahllöf, Göran; Lindahl, Katarina; Kindmark, Andreas; Grigelioniene, Giedre; Åström, Eva; Malmgren, Barbro

    2017-01-01

    Osteogenesis imperfecta (OI) is a heterogeneous group of disorders of connective tissue, caused mainly by mutations in the collagen I genes (COL1A1 and COL1A2). Dentinogenesis imperfecta (DGI) and other dental aberrations are common features of OI. We investigated the association between collagen I mutations and DGI, taurodontism, and retention of permanent second molars in a retrospective cohort of 152 unrelated children and adolescents with OI. The clinical examination included radiographic evaluations. Teeth from 81 individuals were available for histopathological evaluation. COL1A1/2 mutations were found in 104 individuals by nucleotide sequencing. DGI was diagnosed clinically and radiographically in 29% of the individuals (44/152) and through isolated histological findings in another 19% (29/152). In the individuals with a COL1A1 mutation, 70% (7/10) of those with a glycine substitution located C-terminal of p.Gly305 exhibited DGI in both dentitions while no individual (0/7) with a mutation N-terminal of this point exhibited DGI in either dentition (p = 0.01). In the individuals with a COL1A2 mutation, 80% (8/10) of those with a glycine substitution located C terminal of p.Gly211 exhibited DGI in both dentitions while no individual (0/5) with a mutation N-terminal of this point (p = 0.007) exhibited DGI in either dentition. DGI was restricted to the deciduous dentition in 20 individuals. Seventeen had missense mutations where glycine to serine was the most prevalent substitution (53%). Taurodontism occurred in 18% and retention of permanent second molars in 31% of the adolescents. Dental aberrations are strongly associated with qualitatively changed collagen I. The varying expressivity of DGI is related to the location of the collagen I mutation. Genotype information may be helpful in identifying individuals with OI who have an increased risk of dental aberrations. PMID:28498836

  7. ColVI myopathies: where do we stand, where do we go?

    Directory of Open Access Journals (Sweden)

    Allamand Valérie

    2011-09-01

    Full Text Available Abstract Collagen VI myopathies, caused by mutations in the genes encoding collagen type VI (ColVI, represent a clinical continuum with Ullrich congenital muscular dystrophy (UCMD and Bethlem myopathy (BM at each end of the spectrum, and less well-defined intermediate phenotypes in between. ColVI myopathies also share common features with other disorders associated with prominent muscle contractures, making differential diagnosis difficult. This group of disorders, under-recognized for a long time, has aroused much interest over the past decade, with important advances made in understanding its molecular pathogenesis. Indeed, numerous mutations have now been reported in the COL6A1, COL6A2 and COL6A3 genes, a large proportion of which are de novo and exert dominant-negative effects. Genotype-phenotype correlations have also started to emerge, which reflect the various pathogenic mechanisms at play in these disorders: dominant de novo exon splicing that enables the synthesis and secretion of mutant tetramers and homozygous nonsense mutations that lead to premature termination of translation and complete loss of function are associated with early-onset, severe phenotypes. In this review, we present the current state of diagnosis and research in the field of ColVI myopathies. The past decade has provided significant advances, with the identification of altered cellular functions in animal models of ColVI myopathies and in patient samples. In particular, mitochondrial dysfunction and a defect in the autophagic clearance system of skeletal muscle have recently been reported, thereby opening potential therapeutic avenues.

  8. Oil spill response issues in Washington State

    International Nuclear Information System (INIS)

    Lempriere, P.R.

    1997-01-01

    Washington State statutes and regulations applicable to oil transport and oil spills were described. Specific provisions of the statutes and regulations and other relevant matters were also discussed. Among these were: (1) Washington State oil spill prevention plans, (2) Washington State oil spill contingency plans, (3) best achievable protection, (4) Intertanko's lawsuit against Washington State, (5) oil spill removal organizations, (6) certificates of financial responsibility in Washington State, (7) extent of potential liability under Washington Law, (8) disposal of cleanup materials, and (9) definition of 'qualified individuals' on marine vessels having the authority to implement removal actions

  9. A COL11A2 mutation in Labrador retrievers with mild disproportionate dwarfism.

    Directory of Open Access Journals (Sweden)

    Mirjam Frischknecht

    Full Text Available We describe a mild form of disproportionate dwarfism in Labrador Retrievers, which is not associated with any obvious health problems such as secondary arthrosis. We designate this phenotype as skeletal dysplasia 2 (SD2. It is inherited as a monogenic autosomal recessive trait with incomplete penetrance primarily in working lines of the Labrador Retriever breed. Using 23 cases and 37 controls we mapped the causative mutation by genome-wide association and homozygosity mapping to a 4.44 Mb interval on chromosome 12. We re-sequenced the genome of one affected dog at 30x coverage and detected 92 non-synonymous variants in the critical interval. Only two of these variants, located in the lymphotoxin A (LTA and collagen alpha-2(XI chain gene (COL11A2, respectively, were perfectly associated with the trait. Previously described COL11A2 variants in humans or mice lead to skeletal dysplasias and/or deafness. The dog variant associated with disproportionate dwarfism, COL11A2:c.143G>C or p.R48P, probably has only a minor effect on collagen XI function, which might explain the comparatively mild phenotype seen in our study. The identification of this candidate causative mutation thus widens the known phenotypic spectrum of COL11A2 mutations. We speculate that non-pathogenic COL11A2 variants might even contribute to the heritable variation in height.

  10. A COL11A2 mutation in Labrador retrievers with mild disproportionate dwarfism.

    Science.gov (United States)

    Frischknecht, Mirjam; Niehof-Oellers, Helena; Jagannathan, Vidhya; Owczarek-Lipska, Marta; Drögemüller, Cord; Dietschi, Elisabeth; Dolf, Gaudenz; Tellhelm, Bernd; Lang, Johann; Tiira, Katriina; Lohi, Hannes; Leeb, Tosso

    2013-01-01

    We describe a mild form of disproportionate dwarfism in Labrador Retrievers, which is not associated with any obvious health problems such as secondary arthrosis. We designate this phenotype as skeletal dysplasia 2 (SD2). It is inherited as a monogenic autosomal recessive trait with incomplete penetrance primarily in working lines of the Labrador Retriever breed. Using 23 cases and 37 controls we mapped the causative mutation by genome-wide association and homozygosity mapping to a 4.44 Mb interval on chromosome 12. We re-sequenced the genome of one affected dog at 30x coverage and detected 92 non-synonymous variants in the critical interval. Only two of these variants, located in the lymphotoxin A (LTA) and collagen alpha-2(XI) chain gene (COL11A2), respectively, were perfectly associated with the trait. Previously described COL11A2 variants in humans or mice lead to skeletal dysplasias and/or deafness. The dog variant associated with disproportionate dwarfism, COL11A2:c.143G>C or p.R48P, probably has only a minor effect on collagen XI function, which might explain the comparatively mild phenotype seen in our study. The identification of this candidate causative mutation thus widens the known phenotypic spectrum of COL11A2 mutations. We speculate that non-pathogenic COL11A2 variants might even contribute to the heritable variation in height.

  11. COL Application Content Guide for HTGRs: Revision to RG 1.206, Part 1 - Status Report

    Energy Technology Data Exchange (ETDEWEB)

    Wayne Moe

    2012-08-01

    A combined license (COL) application is required by the Nuclear Regulatory Commission (NRC) for all proposed nuclear plants. The information requirements for a COL application are set forth in 10 CFR 52.79, “Contents of Applications; Technical Information in Final Safety Analysis Report.” An applicant for a modular high temperature gas-cooled reactor (HTGR) must develop and submit for NRC review and approval a COL application which conforms to these requirements. The technical information necessary to allow NRC staff to evaluate a COL application and resolve all safety issues related to a proposed nuclear plant is detailed and comprehensive. To this, Regulatory Guide (RG) 1.206, “Combined License Applications for Nuclear Power Plants” (LWR Edition), was developed to assist light water reactor (LWR) applicants in incorporating and effectively formatting required information for COL application review (Ref. 1). However, the guidance prescribed in RG 1.206 presumes a LWR design proposal consistent with the systems and functions associated with large LWR power plants currently operating under NRC license.

  12. Case of Small Vessel Disease Associated with COL4A1 Mutations following Trauma

    Directory of Open Access Journals (Sweden)

    Joao McONeil Plancher

    2015-06-01

    Full Text Available With this case report, we would like to heighten the awareness of clinicians about COL4A1 as a single-gene disorder causing cerebral small vessel disease and describe a previously unreported pathogenic missense substitution in COL4A1 (p.Gly990Val and a new clinical presentation. We identified a heterozygous putatively pathogenic mutation of COL4A1 in a 50-year-old female with a history of congenital cataracts and glaucoma who presented with multiple diffusion-positive infarcts and areas of contrast enhancement following mild head trauma. We believe that this presentation of multiple areas of acute brain and vascular injury in the setting of mild head trauma is a new manifestation of this genetic disorder. Imaging findings of multiple acute infarcts and regions of contrast enhancement with associated asymptomatic old deep microhemorrhages and leukomalacia in adults after head trauma should raise a high suspicion for a COL4A1 genetic disorder. Radiographic patterns of significant leukoaraiosis and deep microhemorrhages can also be seen in patients with long-standing vasculopathy associated with hypertension, which our patient lacked. Our findings demonstrate the utility of genetic screening for COL4A1 mutations in young patients who have small vessel vasculopathy on brain imaging but who do not have significant cardiovascular risk factors.

  13. Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNA

    Science.gov (United States)

    Hershfield, Vickers; Boyer, Herbert W.; Yanofsky, Charles; Lovett, Michael A.; Helinski, Donald R.

    1974-01-01

    DNA fragments obtained from EcoRI endonuclease digestion of bacteriophage ϕ80pt190 (trp+) and the plasmid ColE1 were covalently joined with polynucleotide ligase. Transformation of Escherichia coli trp- strains to tryptophan independence with the recombined DNA selected for reconstituted ColE1 plasmids containing the tryptophan operon and the ϕ80 immunity region. Similarly, an EcoRI endonuclease generated fragment of plasmid pSC105 DNA containing the genetic determinant of kanamycin resistance was inserted into the ColE1 plasmid and recovered in E. coli. The plasmids containing the trp operon (ColE1-trp) and the kanamycin resistance gene were maintained under logarithmic growth conditions at a level of 25-30 copies per cell and accumulate to the extent of several hundred copies per cell in the presence of chloramphenicol. Cells carrying the ColE1-trp plasmid determined the production of highly elevated levels of trp operon-specific mRNA and tryptophan biosynthetic enzymes. Images PMID:4610576

  14. Abortive phage-infection and UV-protection markers on ColI plasmids from epidemic strains of Salmonella

    International Nuclear Information System (INIS)

    Barker, R.M.

    1988-01-01

    Cultures of Escherichia coli carrying ColI plasmids received in conjugation from strains of Salmonella typhimurium and S. agona were examined for abortive infection (Abi) of phage BF23 and for enhanced resistance to the lethal action of UV-irradiation (Uvr). The Abi character of stored cultures of E. coli was also compared with the reaction of the same stock culture tested 5 years before. Seven of the eight potential types differentiated by three characters were represented among 160 ColI plasmids: ColIa Abi + Uvr + (3 plasmids), ColIa Abi - Uvr + (1), ColIa Abi - Uvr-> (2), ColIb Abi + Uvr + (85), ColIb Abi + Uvr - (5), ColIb Abi - Uvr + (4), ColIb Abi-? Uvr - (60). Recognition that different plasmid types could be carried by strains of a clone proved useful in the interpretation of the epidemic spread of strains of S. typhimurium of phage type/biotype 141/9f in Scotland and in tracing the ancestry of a recently emerged rhamnose non-fermenting mutant strain of S. agona. (author)

  15. Residential Energy Efficiency Potential: Washington

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, Eric J [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-11-27

    Energy used by Washington single-family homes that can be saved through cost-effective improvements. Prepared by Eric Wilson and Noel Merket, NREL, and Erin Boyd, U.S. Department of Energy Office of Energy Policy and Systems Analysis.

  16. A novel COL11A1 missense mutation in siblings with non-ocular Stickler syndrome.

    Science.gov (United States)

    Kohmoto, Tomohiro; Tsuji, Atsumi; Morita, Kei-Ichi; Naruto, Takuya; Masuda, Kiyoshi; Kashimada, Kenichi; Enomoto, Keisuke; Morio, Tomohiro; Harada, Hiroyuki; Imoto, Issei

    2016-01-01

    Stickler syndrome (STL) is an autosomal, dominantly inherited, clinically variable and genetically heterogeneous connective tissue disorder characterized by ocular, auditory, orofacial and skeletal abnormalities. We conducted targeted resequencing using a next-generation sequencer for molecular diagnosis of a 2-year-old girl who was clinically suspected of having STL with Pierre Robin sequence. We detected a novel heterozygous missense mutation, NM_001854.3:n.4838G>A [NM_001854.3 (COL11A1_v001):c.4520G>A], in COL11A1, resulting in a Gly to Asp substitution at position 1507 [NM_001854.3(COL11A1_i001)] within one of the collagen-like domains of the triple helical region. The same mutation was detected in her 4-year-old brother with cleft palate and high-frequency sensorineural hearing loss.

  17. Washington Tsunami Hazard Mitigation Program

    Science.gov (United States)

    Walsh, T. J.; Schelling, J.

    2012-12-01

    Washington State has participated in the National Tsunami Hazard Mitigation Program (NTHMP) since its inception in 1995. We have participated in the tsunami inundation hazard mapping, evacuation planning, education, and outreach efforts that generally characterize the NTHMP efforts. We have also investigated hazards of significant interest to the Pacific Northwest. The hazard from locally generated earthquakes on the Cascadia subduction zone, which threatens tsunami inundation in less than hour following a magnitude 9 earthquake, creates special problems for low-lying accretionary shoreforms in Washington, such as the spits of Long Beach and Ocean Shores, where high ground is not accessible within the limited time available for evacuation. To ameliorate this problem, we convened a panel of the Applied Technology Council to develop guidelines for construction of facilities for vertical evacuation from tsunamis, published as FEMA 646, now incorporated in the International Building Code as Appendix M. We followed this with a program called Project Safe Haven (http://www.facebook.com/ProjectSafeHaven) to site such facilities along the Washington coast in appropriate locations and appropriate designs to blend with the local communities, as chosen by the citizens. This has now been completed for the entire outer coast of Washington. In conjunction with this effort, we have evaluated the potential for earthquake-induced ground failures in and near tsunami hazard zones to help develop cost estimates for these structures and to establish appropriate tsunami evacuation routes and evacuation assembly areas that are likely to to be available after a major subduction zone earthquake. We intend to continue these geotechnical evaluations for all tsunami hazard zones in Washington.

  18. 78 FR 15053 - Simpson Lumber Company, LLC, Shelton, Washington; Simpson Lumber Company, LLC, Tacoma, Washington...

    Science.gov (United States)

    2013-03-08

    ...,372B] Simpson Lumber Company, LLC, Shelton, Washington; Simpson Lumber Company, LLC, Tacoma, Washington; Simpson Lumber Company, LLC, Longview, Washington; Notice of Revised Determination on Reconsideration On... Reconsideration for the workers and former workers of Simpson Lumber Company, LLC, Shelton, Washington (TA-W-81...

  19. Receptores do factor estimulante de colónias de macrófagos do robalo

    OpenAIRE

    Oliveira, Márcio Adriano Guiomar de

    2010-01-01

    O receptor do factor estimulante de colónias de macrófagos, também conhecido como receptor do factor estimulante de colónias-1 (CSF1R), é um receptor de um factor de crescimento hematopoiético que é especificamente expresso em células do sistema fagocítico-mononuclear e desempenha um papel essencial no desenvolvimento e regulação destas células. O CSF1R já foi descrito em vários mamíferos e a sua biologia tem sido exaustivamente caracterizada nestes vertebrados mas o conheci...

  20. RNase H and replication of ColE1 DNA in Escherichia coli.

    OpenAIRE

    Naito, S; Uchida, H

    1986-01-01

    Amber mutations within the rnh (RNase H) gene of Escherichia coli K-12 were isolated by selecting for bacteria capable of replicating in a sup+ background replication-defective cer-6 mutant of the ColE1 replicon. The cer-6 mutation is an alteration of one base pair located 160 nucleotides upstream of the unique replication origin of this plasmid. Subsequently, we determined the DNA alterations present within these mutants. ColE1 DNA replicated in rnh(Am) recA cells, indicating that (i) RNase ...

  1. Overexpression of COL11A1 by cancer-associated fibroblasts: clinical relevance of a stromal marker in pancreatic cancer.

    Directory of Open Access Journals (Sweden)

    Carmen García-Pravia

    Full Text Available BACKGROUND: The collagen11A1 (COL11A1 gene is overexpressed in pancreatic cancer. The expression of COL11A1 protein could be involved in desmoplastic events in pancreatic cancer, but an antibody that specifically stains the COL11A1 protein is not currently available. METHODS AND FINDINGS: A total of 54 pancreatic ductal adenocarcinomas (PDAC, 23 chronic pancreatitis (CP samples, and cultured peritumoral stromal cells of PDAC (passages 3-6 were studied. Normal human pancreas tissue samples were obtained through a cadaveric organ donation program. 1 Validation of COL11A1 gene overexpression by q-RT-PCR. FINDINGS: the expression of COL11A1 gene is significantly increased in PDAC samples vs. normal and CP samples. 2 Analysis of COL11A1 by immunohistochemistry using highly specific anti-proCOL11A1 antibodies. FINDINGS: anti-proCOL11A1 stains stromal cells/cancer-associated fibroblasts (CAFs of PDAC but it does not stain chronic benign condition (chronic pancreatitis stromal cells, epithelial cells, or normal fibroblasts. 3 Evaluation of the discrimination ability of the antibody. FINDINGS: anti-proCOL11A1 immunostaining accurately discriminates between PDAC and CP (AUC 0.936, 95% CI 0.851, 0.981. 4 Phenotypic characterization of proCOL11A1+ stromal cells co-staining with mesenchymal, epithelial and stellate cell markers on pancreatic tissue samples and cultured peritumoral pancreatic cancer stromal cells. FINDINGS: ProCOL11A1+ cells present co-staining with mesenchymal, stellate and epithelial markers (EMT phenotype in different proportions. CONCLUSIONS/SIGNIFICANCE: Detection of proCOL11A1 through immunostaining with this newly-developed antibody allows for a highly accurate distinction between PDAC and CP. Unlike other available antibodies commonly used to detect CAFs, anti-proCOL11A1 is negative in stromal cells of the normal pancreas and almost absent in benign inflammation. These results strongly suggest that proCOL11A1 is a specific marker for

  2. Father Secchi Goes to Washington

    Science.gov (United States)

    McCarthy, M. F.

    1994-12-01

    In 1848 a small group of Jesuit refugees arrived at Georgetown College near Washington, D.C. Among them was a young priest, Angelo Secchi, who had finished theology studies in Rome, but had not been able to complete his final examinations. This done successfully, Secchi turned to astronomy and the new facilities of the Georgetown College Observatory, directed by its founder, Fr. James Curley. During his two years in Washington, Secchi studied physics, wrote an article on Electrical Rheometry for the Smithsonian Institution, and formed a friendship with Matthew Fontaine Maury of the U.S. Navy, who headed the Chart Service and in 1844 was named superintendent of the National Observatory. This was later named the U.S. Naval Observatory. Secchi's friendships formed during the Washington visit proved most helpful for relations between European astronomers and U.S. colleagues. Secchi, after his return to Rome constructed the Observatory of the Collegio Romano atop the baroque Church of St. Ignatius in Rome and began his work in spectral classification of stars.

  3. Severe Hemolytic Jaundice in a Neonate with a Novel COL4A1 Mutation.

    Science.gov (United States)

    Tomotaki, Seiichi; Mizumoto, Hiroshi; Hamabata, Takayuki; Kumakura, Akira; Shiota, Mitsutaka; Arai, Hiroshi; Haginoya, Kazuhiro; Hata, Daisuke

    2016-12-01

    We report our experience with a preterm infant with severe hemolytic jaundice who required exchange transfusion just after birth. The patient was negative for alloimmune hemolysis as a result of maternal-fetal blood type incompatibility, and tests for inherited defects in erythrocyte metabolism, membrane function, and hemoglobin synthesis were normal. We also performed a bone marrow examination, but could not identify the cause of hemolysis. The patient had several other complications, including porencephaly, epilepsy, elevated serum levels of creatine kinase, and persistent microscopic hematuria. Later, we detected a genetic mutation in COL4A1, which was recently found to be associated with hemolytic anemia. We therefore believe that all of the patient's clinical features, including hemolytic anemia, were due to the mutation in COL4A1. Genetic testing for COL4A1 mutations is recommended in neonates who exhibit hemolytic disease of unknown etiology, especially when other complications compatible with COL4A1-related disorders are present. Copyright © 2014. Published by Elsevier B.V.

  4. DICE/ColDICE: 6D collisionless phase space hydrodynamics using a lagrangian tesselation

    Science.gov (United States)

    Sousbie, Thierry

    2018-01-01

    DICE is a C++ template library designed to solve collisionless fluid dynamics in 6D phase space using massively parallel supercomputers via an hybrid OpenMP/MPI parallelization. ColDICE, based on DICE, implements a cosmological and physical VLASOV-POISSON solver for cold systems such as dark matter (CDM) dynamics.

  5. Effects of a malfunctional column on conventional and FeedCol-simulated moving bed chromatography performance.

    Science.gov (United States)

    Song, Ji-Yeon; Oh, Donghoon; Lee, Chang-Ha

    2015-07-17

    The effects of a malfunctional column on the performance of a simulated moving bed (SMB) process were studied experimentally and theoretically. The experimental results of conventional four-zone SMB (2-2-2-2 configuration) and FeedCol operation (2-2-2-2 configuration with one feed column) with one malfunctional column were compared with simulation results of the corresponding SMB processes with a normal column configuration. The malfunctional column in SMB processes significantly deteriorated raffinate purity. However, the extract purity was equivalent or slightly improved compared with the corresponding normal SMB operation because the complete separation zone of the malfunctional column moved to a lower flow rate range in zones II and III. With the malfunctional column configuration, FeedCol operation gave better experimental performance (up to 7%) than conventional SMB operation because controlling product purity with FeedCol operation was more flexible through the use of two additional operating variables, injection time and injection length. Thus, compared with conventional SMB separation, extract with equivalent or slightly better purity could be produced from FeedCol operation even with a malfunctional column, while minimizing the decrease in raffinate purity (less than 2%). Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Las cuentas del cuarto viaje de Cristóbal Colón

    Directory of Open Access Journals (Sweden)

    Gil, Juan

    2003-12-01

    Full Text Available The payroll of the fourth voyage of Christopher Columbus is here published for the fisrt time, signed by Admiral himself. A commentary is also furnished.

    Se publican por primera vez, con comentario, las cuentas del cuarto viaje de Cristóbal Colón, firmadas por el propio almirante.

  7. El almirante Cristóbal Colón y la ciudad de Valladolid

    OpenAIRE

    Varela Marcos , Jesús

    2006-01-01

    Pretendemos aunar todas las presencias de Colón en la ciudad de Valladolid en el año de la conmemoración de su muerte. Para ello hemos rastreado en la documentación todos los momentos en que el genovés estuvo en dicha villa.

  8. Views of Astronaut (Col.) Joe Engle and son Jon with L-5 Piper Cub

    Science.gov (United States)

    1981-01-01

    Views of Astronaut (Col.) Joe Engle and son Jon with L-5 Piper Cub at Clover Airport. Photos include Engle turning propeller while his son sits in the cockpit (34323); both Engle and son examine propeller (34324); Engle works on engine while his son sits in cockpit (34325).

  9. Wound healing genes and susceptibility to cutaneous leishmaniasis in Brazil: Role of COL1A1

    Science.gov (United States)

    Almeida, Lucas; Oliveira, Joyce; Guimarães, Luiz Henrique; Carvalho, Edgar M; Blackwell, Jenefer M

    2015-01-01

    Previous studies have demonstrated a role for wound healing genes in resolution of cutaneous lesions caused by Leishmania spp. in both mice and humans, including the gene FLI1 encoding Friend leukaemia virus integration 1. Reduction of Fli1 expression in mice has been shown to result in up-regulation of collagen type I alpha 1 (Col1a1) and alpha 2 (Col1a2) genes and, conversely, in down-regulation of the matrix metalloproteinase 1 (Mmp1) gene, suggesting that Fli1 suppression is involved in activation of the profibrotic gene program. Here we examined single nucleotide polymorphisms (SNPs) in these genes as risk factors for cutaneous (CL) and mucosal leishmaniasis (ML), and leishmaniasis per se, caused by L. braziliensis in humans. SNPs were genotyped in 168 nuclear families (250 CL; 87 ML cases) and replicated in 157 families (402 CL; 39 ML cases). Family-based association tests (FBAT) showed the strongest association between SNPs rs1061237 (combined P=0.002) and rs2586488 (combined P=0.027) at COL1A1 and CL disease. This contributes to our further understanding of the role of wound healing in the resolution of CL disease, providing potential for therapies modulating COL1A1 via drugs acting on FLI1. PMID:25562121

  10. Population haplotype analysis and evolutionary relations of the COL2A1 gene

    NARCIS (Netherlands)

    Meulenbelt, I.; Williams, G.J.; Koppele, J.M.T.E.; Giessen, G.C.D.E. van; Slagboom, P.E.

    1996-01-01

    We have determined the allele frequencies mad pairwise linkage disequilibria of restriction fragment length polymorphisms (RFLPs) distributed over the entire COL2A1 gene (spanning 23 · 6 kb) in a population of unrelated Dutch Caucasians. Pairwise linkage disequilibrium analysis of RFLP sites between

  11. Two-step method for curing Escherichia coli of ColE1-derived plasmids

    DEFF Research Database (Denmark)

    Hove-Jensen, Bjarne

    2008-01-01

    To cure Escherichia coli for plasmids derived from the ColE1 replicon advantage is taken of the fact that maintenance of this replicon requires a wild-type allele of polA, encoding DNA polymerase I. Curing is achieved by cotransduction of a mutant polA allele with metE::Tn10, fadAB::Tn10 or other...

  12. A novel COL4A3 mutation causes autosomal-recessive Alport syndrome in a large Turkish family.

    Science.gov (United States)

    Uzak, Asli Subasioglu; Tokgoz, Bulent; Dundar, Munis; Tekin, Mustafa

    2013-03-01

    Alport syndrome (AS) is a genetically heterogeneous disorder that is characterized by hematuria, progressive renal failure typically resulting in end-stage renal disease, sensorineural hearing loss, and variable ocular abnormalities. Only 15% of cases with AS are autosomal recessive and are caused by mutations in the COL4A3 or COL4A4 genes, encoding type IV collagen. Clinical data in a large consanguineous family with four affected members were reviewed, and genomic DNA was extracted. For mapping, 15 microsatellite markers flanking COL4A3, COL4A4, and COL4A5 in 16 family members were typed. For mutation screening, all coding exons of COL4A3 were polymerase chain reaction- amplified and Sanger-sequenced from genomic DNA. The disease locus was mapped to chromosome 2q36.3, where COL4A3 and COL4A4 reside. Sanger sequencing revealed a novel mis-sense mutation (c.2T>C; p.M1T) in exon 1 of COL4A3. The identified nucleotide change was not found in 100 healthy ethnicity-matched controls via Sanger sequencing. We present a large consanguineous Turkish family with AS that was found to have a COL4A3 mutation as the cause of the disease. Although the relationship between the various genotypes and phenotypes in AS has not been fully elucidated, detailed clinical and molecular analyses are helpful for providing data to be used in genetic counseling. It is important to identify new mutations to clarify their clinical importance, to assess the prognosis of the disease, and to avoid renal biopsy for final diagnosis.

  13. TUMOR-SPECIFIC EXPRESSION AND ALTERNATIVE SPLICING OF THE COL6A3 GENE IN PANCREATIC CANCER

    Science.gov (United States)

    Arafat, Hwyda; Lazar, Melissa; Salem, Khalifa; Chipitsyna, Galina; Gong, Qiaoke; Pan, Te-Cheng; Zhang, Rui-Zhu; Yeo, Charles J.; Chu, Mon-Li

    2011-01-01

    Introduction Pancreatic ductal adenocarcinoma (PDA) is a highly lethal disease in which a prominent desmoplastic reaction is a defining characteristic. Fibrillar collagens, such as collagen I and to a lesser extent, collagen III and V comprise the majority of this stromal fibrosis. Type VI collagen (COL6) forms a microfibrillar network associated with type I collagen fibrils. The expression of COL6 has been linked to inflammation and survival. Importantly, tumor-specific alternative splicing in COL6A3 has been identified in several cancers by genome exon arrays. We evaluated the expression and localization of COL6A3 in PDA and premalignant lesions and explored the presence of alternative splicing events. Methods We analyzed paired PDA-normal (n=18), IPMN (n=5), pancreatic cystadenoma (n=5), and eight PDA cell lines with RT-PCR, using unique primers that identify total COL6A3 gene and alternative splicing sites in several of its exons. Western blot analysis and immunohistochemistry were used to analyze the expression levels and localization of COL6A3 protein in the different lesions, and in two animal models of PDA. Results COL6A3 protein levels were significantly upregulated in 77% of the paired PDA-adjacent tissue examined. COL6A3 was mainly present in the desmoplastic stroma of PDA, with high deposition around the malignant ducts and in between the sites of stromal fatty infiltration. Analysis of the COL6A3 splice variants showed tumor-specific consistent inclusion of exons 3 and 6 in 17 of the 18 (94%) paired PDA-adjacent tissues. Inclusion of exon 4 was exclusively tumor-specific, with barely detectable expression in the adjacent tissues. IPMN and pancreatic cystadenomas showed no expression of any of the examined exons. Total COL6A3 mRNA and exon 6 were identified in six PDA cell lines, but only two cell lines (MIA PACA-2 and ASPC-1) expressed exons 3 and 4. In both the xenograft and transgenic models of PDA, COL6A3 immunoreactivity was present in the stroma

  14. CHROMagar COL-APSE: a selective bacterial culture medium for the isolation and differentiation of colistin-resistant Gram-negative pathogens

    DEFF Research Database (Denmark)

    Abdul Momin, Muhd Haziq F; Bean, David C; Hendriksen, Rene S.

    2017-01-01

    Purpose. A selective chromogenic culture medium for the laboratory isolation and differentiation of colistin resistant Acinetobacter, Pseudomonas, Stenotrophomonas and Enterobacteriaceae spp. (CHROMagar COL-APSE) was developed, evaluated and compared to an existing selective bacterial culture......-resistant non-fermentative bacteria (Acinetobacter, Pseudomonas and Stenotrophomonas). CHROMagar COL-APSE was also more sensitive in supporting the growth of Enterobacteriaceae with COL resistance associated with the carriage of mcr-1. Conclusion. CHROMagar COL-APSE is a sensitive and specific medium...

  15. Novel COL4A1 mutations associated with HANAC syndrome: a role for the triple helical CB3[IV] domain.

    Science.gov (United States)

    Plaisier, Emmanuelle; Chen, Zhiyong; Gekeler, Florian; Benhassine, Safa; Dahan, Karine; Marro, Béatrice; Alamowitch, Sonia; Paques, Michel; Ronco, Pierre

    2010-10-01

    The COL4A1 gene encodes the α1-chain of type IV collagen, which is ubiquitously expressed in basement membranes. Mutations in COL4A1 have been reported in autosomal-dominant porencephaly and in patients with symptomatic small vessel brain disease, inconstantly associated with eye defects. We have previously reported three COL4A1 mutations associated with a systemic phenotype that we called HANAC (Hereditary Angiopathy, Nephropathy, Aneurysms, and Cramps). We carried out a clinical and genetic study of three families presenting with characteristic features of HANAC syndrome. Common systemic signs included arterial retinal tortuosity and muscle cramps, with a variable combination of small vessel brain disease, Raynaud phenomena, and kidney defects. Three novel COL4A1 missense substitutions are described, which affect highly conserved glycine residues within the collagenous domain of the protein. All six known mutations associated with the HANAC phenotype are localized within the CB3[IV] fragment of COL4A1, which encompasses major integrin-binding sites. Our results confirm that HANAC syndrome is a distinct clinical entity within the COL4A1-related disorders, which is characterized by systemic involvement and usually asymptomatic brain disease. The restricted distribution of COL4A1 mutations within the CB3[IV] region is a characteristic of the reports of patients with HANAC, which suggests that abnormal cell-type IV collagen interactions may underlie the systemic defects observed in this syndrome. Copyright © 2010 Wiley-Liss, Inc.

  16. Washington: a guide to geothermal energy development

    Energy Technology Data Exchange (ETDEWEB)

    Bloomquist, R.G.; Basescu, N.; Higbee, C.; Justus, D.; Simpson, S.

    1980-06-01

    Washington's geothermal potential is discussed. The following topics are covered: exploration, drilling, utilization, legal and institutional setting, and economic factors of direct use projects. (MHR)

  17. Bone mineral properties in growing Col1a2(+/G610C) mice, an animal model of osteogenesis imperfecta.

    Science.gov (United States)

    Masci, Marco; Wang, Min; Imbert, Laurianne; Barnes, Aileen M; Spevak, Lyudmila; Lukashova, Lyudmila; Huang, Yihe; Ma, Yan; Marini, Joan C; Jacobsen, Christina M; Warman, Matthew L; Boskey, Adele L

    2016-06-01

    The Col1a2(+/G610C) knock-in mouse, models osteogenesis imperfecta in a large old order Amish family (OOA) with type IV OI, caused by a G-to-T transversion at nucleotide 2098, which alters the gly-610 codon in the triple-helical domain of the α2(I) chain of type I collagen. Mineral and matrix properties of the long bones and vertebrae of male Col1a2(+/G610C) and their wild-type controls (Col1a2(+/+)), were characterized to gain insight into the role of α2-chain collagen mutations in mineralization. Additionally, we examined the rescuability of the composition by sclerostin inhibition initiated by crossing Col1a2(+/G610C) with an LRP(+/A214V) high bone mass allele. At age 10-days, vertebrae and tibia showed few alterations by micro-CT or Fourier transform infrared imaging (FTIRI). At 2-months-of-age, Col1a2(+/G610C) tibias had 13% fewer secondary trabeculae than Col1a2(+/+), these were thinner (11%) and more widely spaced (20%) than those of Col1a2(+/+) mice. Vertebrae of Col1a2(+/G610C) mice at 2-months also had lower bone volume fraction (38%), trabecular number (13%), thickness (13%) and connectivity density (32%) compared to Col1(a2+/+). The cortical bone of Col1a2(+/G610C) tibias at 2-months had 3% higher tissue mineral density compared to Col1a2(+/+); Col1a2(+/G610C) vertebrae had lower cortical thickness (29%), bone area (37%) and polar moment of inertia (38%) relative to Col1a2(+/+). FTIRI analysis, which provides information on bone chemical composition at ~7μm-spatial resolution, showed tibias at 10-days did not differ between genotypes. Comparing identical bone types in Col1a2(+/G610C) to Col1a2(+/+) at 2-months-of-age, tibias showed higher mineral-to-matrix ratio in trabeculae (17%) and cortices (31%). and in vertebral cortices (28%). Collagen maturity was 42% higher at 10-days-of-age in Col1a2(+/G610C) vertebral trabeculae and in 2-month tibial cortices (12%), vertebral trabeculae (42%) and vertebral cortices (12%). Higher acid-phosphate substitution

  18. Columbian Secrets: Cristóbal Colón by Leonardo Balada and Antonio Gala

    Directory of Open Access Journals (Sweden)

    José Elías Gutiérrez Meza

    2016-11-01

    Full Text Available The play Cristóbal Colón premiered in 1989 at the Teatro del Liceu. Composed by the musician Leonardo Balada and the playwright Antonio Gala, this opera was commissioned by the Sociedad Estatal Quinto Centenario as part of the festivities organized by the Spanish government to commemorate the Discovery of America. Unlike the so-called "des-celebración del 92", Cristóbal Colón champions the figure of the discoverer and emphasizes the participation of Spain through the praised role of Martín Alonso Pinzón. However, the opera was cut at the last minute. This article asserts that such alteration may have been the result of political censorship attempting to counteract the criticism of the Discovery of America contained in the original version.

  19. CHROMagar COL-APSE: a selective bacterial culture medium for the isolation and differentiation of colistin-resistant Gram-negative pathogens

    DEFF Research Database (Denmark)

    Abdul Momin, Muhd Haziq F; Bean, David C; Hendriksen, Rene S.

    2017-01-01

    medium (SuperPolymyxin). Methodology. The medium was challenged with 84 isolates, including polymyxin B (POL B)-susceptible and -resistant type strains and colistin (COL)-resistant organisms recovered from human and animal samples. Susceptibility to COL and POL B was determined by agar dilution and broth...... microtitre dilution. The lower limit for the detection of COL-resistant organisms was also calculated for both CHROMagar COL-APSE and SuperPolymyxin media. The ability to isolate and correctly differentiate COL-resistant organisms within mixed cultures was also assessed and compared using both media. Results...

  20. Purification of supercoiled DNA of plasmid Col E1 by RPC-5 chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Best, A.N.; Allison, D.P.; Novelli, G.D.

    1981-07-01

    Col E1 DNA can be purified to a high degree by RPC-5 chromatography of a partially purified cell lysate with a very shallow linear NaC1 gradient at pH 7.8. Electron micrographs demonstrated that the purest fractions were composed of 93% supercoiled (form I) DNA and 7% open circular (form II) DNA. The actual chromatography can be accomplished in 13 to 14 h and is designed for the production of several milligrams of plasmid DNA.

  1. Colômbia 2010: Guerra, Paz e Eleições

    Directory of Open Access Journals (Sweden)

    Luiz Antônio Gusmão

    2009-02-01

    Full Text Available

    O enfraquecimento das Farc deve polarizar as

    eleições de 2010 na Colômbia entre recrudescimento

    do combate e abertura à negociação.

  2. Loss of col8a1a Function during Zebrafish Embryogenesis Results in Congenital Vertebral Malformations

    Science.gov (United States)

    Gray, Ryan S.; Wilm, Thomas; Smith, Jeff; Bagnat, Michel; Dale, Rodney M.; Topczewski, Jacek; Johnson, Stephen L.; Solnica-Krezel, Lilianna

    2014-01-01

    Congenital vertebral malformations (CVM) occur in 1 in 1,000 live births and in many cases can cause spinal deformities, such as scoliosis, and result in disability and distress of affected individuals. Many severe forms of the disease, such as spondylocostal dystostosis, are recessive monogenic traits affecting somitogenesis, however the etiologies of the majority of CVM cases remain undetermined. Here we demonstrate that morphological defects of the notochord in zebrafish can generate congenital-type spine defects. We characterize three recessive zebrafish leviathan/col8a1a mutant alleles (m531, vu41, vu105) that disrupt collagen type VIII alpha1a (col8a1a), and cause folding of the embryonic notochord and consequently adult vertebral column malformations. Furthermore, we provide evidence that a transient loss of col8a1a function or inhibition of Lysyl oxidases with drugs during embryogenesis was sufficient to generate vertebral fusions and scoliosis in the adult spine. Using periodic imaging of individual zebrafish, we correlate focal notochord defects of the embryo with vertebral malformations (VM) in the adult. Finally, we show that bends and kinks in the notochord can lead to aberrant apposition of osteoblasts normally confined to well-segmented areas of the developing vertebral bodies. Our results afford a novel mechanism for the formation of VM, independent of defects of somitogenesis, resulting from aberrant bone deposition at regions of misshapen notochord tissue. PMID:24333517

  3. Generation of Col2a1-EGFP iPS cells for monitoring chondrogenic differentiation.

    Directory of Open Access Journals (Sweden)

    Taku Saito

    Full Text Available Induced pluripotent stem cells (iPSC are a promising cell source for cartilage regenerative medicine; however, the methods for chondrocyte induction from iPSC are currently developing and not yet sufficient for clinical application. Here, we report the establishment of a fluorescent indicator system for monitoring chondrogenic differentiation from iPSC to simplify screening for effective factors that induce chondrocytes from iPSC. We generated iPSC from embryonic fibroblasts of Col2a1-EGFP transgenic mice by retroviral transduction of Oct4, Sox2, Klf4, and c-Myc. Among the 30 clones of Col2a1-EGFP iPSC we established, two clones showed high expression levels of embryonic stem cell (ESC marker genes, similar to control ESC. A teratoma formation assay showed that the two clones were pluripotent and differentiated into cell types from all three germ layers. The fluorescent signal was observed during chondrogenic differentiation of the two clones concomitant with the increase in chondrocyte marker expression. In conclusion, Col2a1-EGFP iPSC are useful for monitoring chondrogenic differentiation and will contribute to research in cartilage regenerative medicine.

  4. Pertinencia de los postgrados para economistas en el Núcleo LUZ–COL

    Directory of Open Access Journals (Sweden)

    Ana Teresa Prieto Sánchez

    2002-01-01

    Full Text Available El presente artículo tiene como finalidad presentar los resultados obtenidos en un estudio de mercado, cuyo objetivo fue determinar la pertinencia de los postgrados para economistas en el Núcleo LUZ-COL. En dicho estudio se analizaron las expectativas, necesidades y percepciones de los usuarios potenciales del servicio de postgrado, considerando como usuarios potenciales (demanda a los entes empleadores de la COL, docentes de economía en el Núcleo, egresados del programa de economía en el Núcleo y estudiantes del último semestre de la carrera. Estos elementos sirvieron de base para conocer la pertinencia o no del postgrado. La metodología utilizada se fundamentó en una investigación descriptiva y cualitativa, en donde se utilizó el cuestionario como instrumento, para recabar la información primaria. Los resultados muestran una alta necesidad de los programas de postgrados para economistas y delimitan una demanda potencial considerable. El análisis permitió concluir que sí son pertinentes los postgrados para economistas en el Núcleo COL, en las áreas de gerencia de empresas, formulación y evaluación de proyectos, costos o finanzas; del tipo maestría o especialización profesional. En virtud de los resultados, se recomienda, desarrollar estrategias viables que permitan a los tomadores de decisión implementar el postgrado para economistas en el Núcleo.

  5. Stimulation of Escherichia coli F-18Col- Type-1 fimbriae synthesis by leuX

    DEFF Research Database (Denmark)

    Newman, Joseph V.; Burghoff, Robert L.; Pallesen, Lars

    1994-01-01

    Escherichia coli F-18, a normal human fecal isolate, is an excellent colonizer of the streptomycin-treated mouse large intestine. E. coli F-18Col-, a derivative of E. coli F-18 which no longer makes the E. coli F-18 colicin, colonizes the large intestine as well as E. coli F-18 when fed to mice...... alone but is eliminated when fed together with E. coli F-18. Recently we randomly cloned E. coli F-18 DNA into E. coli F-18Col- and let the mouse intestine select the best colonizer. In this way, we isolated a 6.5-kb E. coli F-18 DNA sequence that simultaneously stimulated synthesis of type 1 fimbriae...... and enhanced E. coli F-18Col- colonizing ability. In the present investigation we show that the gene responsible for stimulation of type 1 fimbriae synthesis appears to be leuX, which encodes a tRNA specific for the rare leucine codon UUG. Moreover, it appears that expression of leuX may be regulated by two...

  6. El rol del professor en l’aprenentatge col·laboratiu mediat per ordinador

    Directory of Open Access Journals (Sweden)

    Ingrid Noguera-Fructuoso

    2009-10-01

    Full Text Available En aquest article es presenten els resultats d'una investigació sobre el canvi de rol que es produeix en el professorat en l'ús de les TIC com a eina per a l'aprenentatge col·laboratiu en educació primària i secundària. L'interès es focalitza en l'ús concret d'una eina dissenyada per a l'aprenentatge col·laboratiu, el BSCL, sota el model pedagògic de qüestionament progressiu. En primer lloc, es duu a terme una revisió teòrica. En segon lloc, s'exposen les preguntes d'investigació, la justificació d'aquesta, es descriu el disseny i l'enfocament metodològic escollit, l'estudi de casos. Els resultats obtinguts revelen que hi ha una tendència cap al canvi de rol en el professorat en l'ús del BSCL com a eina per a l'aprenentatge col·laboratiu així com l'eficàcia del model de qüestionament progressiu en la construcció de coneixement de l'alumnat.

  7. Corrections Education. Washington's Community and Technical Colleges

    Science.gov (United States)

    Washington State Board for Community and Technical Colleges, 2015

    2015-01-01

    The Washington State Department of Corrections contracts with community colleges to provide basic education and job training at each of the state's 12 adult prisons so upon release, individuals are more likely to get jobs and less likely to return. Washington State community colleges build a bridge for offenders to successfully re-enter…

  8. Aerospace Training. Washington's Community and Technical Colleges

    Science.gov (United States)

    Washington State Board for Community and Technical Colleges, 2014

    2014-01-01

    Aerospace is an economic powerhouse that generates jobs and fuels our economy. Washington's community and technical colleges produce the world-class employees needed to keep it that way. With about 1,250 aerospace-related firms employing more than 94,000 workers, Washington has the largest concentration of aerospace expertise in the nation. To…

  9. Diferença no tamanho de cascas de ovos de colônias de Panstrongylus megistus

    Directory of Open Access Journals (Sweden)

    Rosa João A da

    2003-01-01

    Full Text Available O objetivo do estudo foi avaliar as mensurações de cascas de ovos de duas colônias laboratoriais e uma silvestre de P. megistus. De cada uma das colônias, 50 cascas de ovos foram mensuradas por meio de microscópio estereoscópico e sistema de análise de imagem QWin. A análise estatística foi efetuada utilizando o teste t-Student, não pareado no programa INSTAT. A largura, o comprimento e o diâmetro da abertura opercular das cascas de ovos de uma colônia silvestre foram maiores que de duas colônias laboratoriais de P. megistus.

  10. Washington State biomass data book

    International Nuclear Information System (INIS)

    Deshaye, J.A.; Kerstetter, J.D.

    1991-07-01

    This is the first edition of the Washington State Biomass Databook. It assess sources and approximate costs of biomass fuels, presents a view of current users, identifies potential users in the public and private sectors, and lists prices of competing energy resources. The summary describes key from data from the categories listed above. Part 1, Biomass Supply, presents data increasing levels of detail on agricultural residues, biogas, municipal solid waste, and wood waste. Part 2, Current Industrial and Commercial Use, demonstrates how biomass is successfully being used in existing facilities as an alternative fuel source. Part 3, Potential Demand, describes potential energy-intensive public and private sector facilities. Part 4, Prices of Competing Energy Resources, shows current suppliers of electricity and natural gas and compares utility company rates. 49 refs., 43 figs., 72 tabs

  11. Evolution of Regions Containing Antibiotic Resistance Genes in FII-2-FIB-1 ColV-Colla Virulence Plasmids.

    Science.gov (United States)

    Moran, Robert A; Hall, Ruth M

    2018-05-01

    Three ColV virulence plasmids carrying antibiotic resistance genes were assembled from draft genome sequences of commensal ST95, ST131, and ST2705 Escherichia coli isolates from healthy Australians. Plasmids pCERC4, pCERC5, and pCERC9 include almost identical backbones containing FII-2 and FIB-1 replicons and the conserved ColV virulence region with an additional ColIa determinant. Only pCERC5 includes a complete, uninterrupted F-like transfer region and was able to conjugate. pCERC5 and pCERC9 contain Tn1721, carrying the tet(A) tetracycline resistance determinant in the same location, with Tn2 (bla TEM ; ampicillin resistance) interrupting the Tn1721 in pCERC5. pCERC4 has a Tn1721/Tn21 hybrid transposon carrying dfrA5 (trimethoprim resistance) and sul1 (sulfamethoxazole resistance) in a class 1 integron. Four FII-2:FIB-1 ColV-ColIa plasmids in the GenBank nucleotide database have a related transposon in the same position, but an IS26 has reshaped the resistance gene region, deleting 2,069 bp of the integron 3'-CS, including sul1, and serving as a target for IS26 translocatable units containing bla TEM , sul2 and strAB (streptomycin resistance), or aphA1 (kanamycin/neomycin resistance). Another ColV-ColIa plasmid containing a related resistance gene region has lost the FII replicon and acquired a unique transfer region via recombination within the resistance region and at oriT. Eighteen further complete ColV plasmid sequences in GenBank contained FIB-1, but the FII replicons were of three types, FII-24, FII-18, and a variant of FII-36.

  12. Volume da gota dos colírios lubrificantes: estudo farmacoeconômico

    Directory of Open Access Journals (Sweden)

    Alexandre Xavier da Costa

    2015-12-01

    Full Text Available RESUMO Objetivo: Determinar o volume médio das gotas produzidas pelos colírios lubrificantes em diferentes ângulos de inclinação e determinar o custo médio do tratamento. Métodos: Determinação do volume da gota de 3 frascos originais dos colírios lubrificantes Artelac®, Hylo Comod®, Lacrima® Plus, Systane® UL, Lacrifilm®, Hyabak®, Lacribell®, Ecofilm®, Mirugell®, Plenigell®, Fresh Tears®, Optive® e Endura® à inclinação de 90º e 45º. Determinou-se o número médio de gotas em cada frasco e foi feita avaliação farmacoeconômica dos colírios. Resultados: O volume das gotas variou de 32,2 a 64,0 µL a 45o e de 29,1 a 65,1 µL a 90o. A diferença entre as gotas em cada inclinação foi de 2 a 24% e o custo anual dos colírios de acordo com a inclinação variou de R$2,73 a R$130,73. A Duração Máxima de Tratamento (DMT foi de 29,3 a 51,4 dias na inclinação de 45o, e de 28,8 a 48,4 dias a 90º, sendo que a diferença na DMT foi de 0,5 até 8 dias a mais ou a menos, de acordo com a marca. Conclusão: Nenhum dos colírios estudados apresentou gotas ideais para o olho humano, levando a um desperdício do produto e maior custo para o fabricante e para o consumidor. Percebemos que existe uma variação significativa no volume da gota de acordo com a inclinação do frasco, e que uma variação maior do que 10% traria impactos financeiros para o paciente.

  13. Over-expression of DSCAM and COL6A2 cooperatively generates congenital heart defects.

    Directory of Open Access Journals (Sweden)

    Tamar R Grossman

    2011-11-01

    Full Text Available A significant current challenge in human genetics is the identification of interacting genetic loci mediating complex polygenic disorders. One of the best characterized polygenic diseases is Down syndrome (DS, which results from an extra copy of part or all of chromosome 21. A short interval near the distal tip of chromosome 21 contributes to congenital heart defects (CHD, and a variety of indirect genetic evidence suggests that multiple candidate genes in this region may contribute to this phenotype. We devised a tiered genetic approach to identify interacting CHD candidate genes. We first used the well vetted Drosophila heart as an assay to identify interacting CHD candidate genes by expressing them alone and in all possible pairwise combinations and testing for effects on rhythmicity or heart failure following stress. This comprehensive analysis identified DSCAM and COL6A2 as the most strongly interacting pair of genes. We then over-expressed these two genes alone or in combination in the mouse heart. While over-expression of either gene alone did not affect viability and had little or no effect on heart physiology or morphology, co-expression of the two genes resulted in ≈50% mortality and severe physiological and morphological defects, including atrial septal defects and cardiac hypertrophy. Cooperative interactions between DSCAM and COL6A2 were also observed in the H9C2 cardiac cell line and transcriptional analysis of this interaction points to genes involved in adhesion and cardiac hypertrophy. Our success in defining a cooperative interaction between DSCAM and COL6A2 suggests that the multi-tiered genetic approach we have taken involving human mapping data, comprehensive combinatorial screening in Drosophila, and validation in vivo in mice and in mammalian cells lines should be applicable to identifying specific loci mediating a broad variety of other polygenic disorders.

  14. Identification and characterization of the human type II collagen gene (COL2A1).

    OpenAIRE

    Cheah, Kathryn; Stoker, N.G.; Griffin, J.R.; Grosveld, Frank; Solomon, E.

    1985-01-01

    textabstractThe gene contained in the human cosmid clone CosHcol1, previously designated an alpha 1(I) collagen-like gene, has now been identified. CosHcol1 hybridizes strongly to a single 5.9-kilobase mRNA species present only in tissue in which type II collagen is expressed. DNA sequence analysis shows that this clone is highly homologous to the chicken alpha 1(II) collagen gene. These data together suggest that CosHcol1 contains the human alpha 1(II) collagen gene COL2A1. The clone appears...

  15. Colágeno asimilable. Fuente de prevención de enfermedades osteoarticuladas

    OpenAIRE

    Parra Parra, Yolanda; Quesada Martínez, María Inmaculada; Pérez Collado, Elisa María

    2015-01-01

    Cartel presentado en la Segunda Conferencia Internacional de Comunicación en Salud, celebrada el 23 de octubre de 2015 en la Universidad Carlos III de Madrid Introducción: El colágeno es la proteína más abundante de nuestro cuerpo humano y uno de sus componentes esencial de articulaciones, cartílago, ligamento, tendones, huesos, piel. Su especial estructura lo hacen único, presenta una estructura fibrosa, que aporta gran resistencia y flexibilidad a los tejidos de los que forma parte. Cu...

  16. A novel COL11A1 mutation affecting splicing in a patient with Stickler syndrome.

    Science.gov (United States)

    Kohmoto, Tomohiro; Naruto, Takuya; Kobayashi, Haruka; Watanabe, Miki; Okamoto, Nana; Masuda, Kiyoshi; Imoto, Issei; Okamoto, Nobuhiko

    2015-01-01

    Stickler syndrome is a clinically and genetically heterogeneous collagenopathy characterized by ocular, auditory, skeletal and orofacial abnormalities, commonly occurring as an autosomal dominant trait. We conducted target resequencing to analyze candidate genes associated with known clinical phenotypes from a 4-year-old girl with Stickler syndrome. We detected a novel heterozygous intronic mutation (NM_001854.3:c.3168+5G>A) in COL11A1 that may impair splicing, which was suggested by in silico prediction and a minigene assay.

  17. A crise Armada Colômbia-Equador no contexto da Guerra contra o Terrorismo Internacional

    Directory of Open Access Journals (Sweden)

    TATIANA WAISBERG

    2010-10-01

    Full Text Available A recente crise armada entre Colômbia e Equador trouxe a América do Sul ao foco da mídia internacional. As FARC representam o maior barril de pólvora da América Latina, além de um determinante ator a ser combatido na luta contra o terrorismo internacional. Nesse sentido, o presente artigo busca analisar, sob o prisma da segurança e do direito internacional o papel das FARC em âmbito global e as maneiras segundo as quais elas são combatidas.

  18. Report : public transportation in Washington State, 1984

    Science.gov (United States)

    1984-10-01

    This report is an update of the Public Transportation in Washington State publication, dated December 1981. In order to reflect the changes that have occurred since that time, this report contains the most current data obtainable. Chapter One of this...

  19. Willapa Bay, Washington Benthic Habitats 1995 Biotic

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In June 1995, the Columbia River Estuary Study Taskforce (CREST) acquired 295 true color aerial photographs (1:12,000) of Willapa Bay, Washington, from the State of...

  20. Willapa Bay, Washington Benthic Habitats 1995 Geoform

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In June 1995, the Columbia River Estuary Study Taskforce (CREST) acquired 295 true color aerial photographs (1:12,000) of Willapa Bay, Washington, from the State of...

  1. Willapa Bay, Washington Benthic Habitats 1995 Substrate

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In June 1995, the Columbia River Estuary Study Taskforce (CREST) acquired 295 true color aerial photographs (1:12,000) of Willapa Bay, Washington, from the State of...

  2. Southwestern Washington 6 arc-second DEM

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The 6-second Southwest Washington Elevation Grid provides bathymetric data in ASCII raster format of 6-second resolution in geographic coordinates. This grid is...

  3. Timber resource statistics for southwest Washington.

    Science.gov (United States)

    Patricia M. Bassett; Daniel D. Oswald

    1981-01-01

    This report summarizes a 1978 timber-resource inventory of six counties in southwest Washington: Clark, Cowlitz, Lewis, Pacific, Skamania, and Wahkiakum. Detailed tables of forest area, timber volume, growth, mortality, and harvest are presented.

  4. Timber resource statistics for eastern Washington.

    Science.gov (United States)

    Patricia M. Bassett; Daniel D. Oswald

    1983-01-01

    This report summarizes a 1980 timber resource inventory of the 16 forested counties in Washington east of the crest of the Cascade Range. Detailed tables of forest area, timber volume, growth, mortality, and harvest are presented.

  5. NSA Diana Wueger Published in Washington Quarterly

    OpenAIRE

    Grant, Catherine L.

    2016-01-01

    National Security Affairs (NSA) News NSA Faculty Associate for Research Diana Wueger has recently had an article titled “India’s Nuclear-Armed Submarines: Deterrence or Danger?” published in the Washington Quarterly.

  6. Loss of Col3a1, the gene for Ehlers-Danlos syndrome type IV, results in neocortical dyslamination.

    Directory of Open Access Journals (Sweden)

    Sung-Jin Jeong

    Full Text Available It has recently been discovered that Collagen III, the encoded protein of the type IV Ehlers-Danlos Syndrome (EDS gene, is one of the major constituents of the pial basement membrane (BM and serves as the ligand for GPR56. Mutations in GPR56 cause a severe human brain malformation called bilateral frontoparietal polymicrogyria, in which neurons transmigrate through the BM causing severe mental retardation and frequent seizures. To further characterize the brain phenotype of Col3a1 knockout mice, we performed a detailed histological analysis. We observed a cobblestone-like cortical malformation, with BM breakdown and marginal zone heterotopias in Col3a1⁻/⁻ mouse brains. Surprisingly, the pial BM appeared intact at early stages of development but starting as early as embryonic day (E 11.5, prominent BM defects were observed and accompanied by neuronal overmigration. Although collagen III is expressed in meningeal fibroblasts (MFs, Col3a1⁻/⁻ MFs present no obvious defects. Furthermore, the expression and posttranslational modification of α-dystroglycan was undisturbed in Col3a1⁻/⁻ mice. Based on the previous finding that mutations in COL3A1 cause type IV EDS, our study indicates a possible common pathological pathway linking connective tissue diseases and brain malformations.

  7. A Gene Gun-mediated Nonviral RNA trans-splicing Strategy for Col7a1 Repair

    Directory of Open Access Journals (Sweden)

    Patricia Peking

    2016-01-01

    Full Text Available RNA trans-splicing represents an auspicious option for the correction of genetic mutations at RNA level. Mutations within COL7A1 causing strong reduction or absence of type VII collagen are associated with the severe skin blistering disease dystrophic epidermolysis bullosa. The human COL7A1 mRNA constitutes a suitable target for this RNA therapy approach, as only a portion of the almost 9 kb transcript has to be delivered into the target cells. Here, we have proven the feasibility of 5′ trans-splicing into the Col7a1 mRNA in vitro and in vivo. We designed a 5′ RNA trans-splicing molecule, capable of replacing Col7a1 exons 1–15 and verified it in a fluorescence-based trans-splicing model system. Specific and efficient Col7a1 trans-splicing was confirmed in murine keratinocytes. To analyze trans-splicing in vivo, we used gene gun delivery of a minicircle expressing a FLAG-tagged 5′ RNA trans-splicing molecule into the skin of wild-type mice. Histological and immunofluorescence analysis of bombarded skin sections revealed vector delivery and expression within dermis and epidermis. Furthermore, we have detected trans-spliced type VII collagen protein using FLAG-tag antibodies. In conclusion, we describe a novel in vivo nonviral RNA therapy approach to restore type VII collagen expression for causative treatment of dystrophic epidermolysis bullosa.

  8. COL-3, a chemically modified tetracycline, inhibits lipopolysaccharide-induced microglia activation and cytokine expression in the brain.

    Directory of Open Access Journals (Sweden)

    Rawan Abdulhameed Edan

    Full Text Available Microglia activation results in release of proinflammatory molecules including cytokines, which contribute to neuronal damage in the central nervous system (CNS if not controlled. Tetracycline antibiotics such as minocycline inhibit microglial activation and cytokine expression during CNS inflammation. In the present study we found that administration of chemically modified tetracycline-3 (COL-3, inhibits lipopolysaccharide (LPS-induced microglial and p38 MAPK activation, as well as the increase in TNF-α, but not IL-1β expression, in the brains of BALB/c mice. COL-3 has been described to have no antibacterial activity. We observed that COL-3 had no activity against a Gram-negative bacteria, Escherichia coli; however surprisingly, COL-3 had antibacterial activity against a Gram-positive bacteria Staphylococcus aureus, with a minimum inhibitory concentration of 1 mg/ml. Our data show that COL-3 has some antibacterial activity against S. aureus, inhibits LPS-induced neuroinflammation, and displays potential as a therapeutic agent for treatment of conditions involving CNS inflammation.

  9. PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene Capture Platforms

    OpenAIRE

    Manuel Ares-Arroyo; Cristina Bernabe-Balas; Alfonso Santos-Lopez; Maria R. Baquero; Kashi N. Prasad; Dolores Cid; Carmen Martin-Espada; Alvaro San Millan; Bruno Gonzalez-Zorn

    2018-01-01

    ColE1 plasmids are important vehicles for the spread of antibiotic resistance in the Enterobacteriaceae and Pasteurellaceae families of bacteria. Their monitoring is essential, as they harbor important resistant determinants in humans, animals and the environment. In this work, we have analyzed ColE1 replicons using bioinformatic and experimental approaches. First, we carried out a computational study examining the structure of different ColE1 plasmids deposited in databases. Bioinformatic an...

  10. Auto-imunidade e colágeno V Autoimmunity and collagen V

    Directory of Open Access Journals (Sweden)

    Cristiane Carla de Oliveira

    2006-06-01

    Full Text Available As proteínas da matriz extracelular (MEC e seus componentes estão sendo amplamente estudados na literatura médica, assim como sua relação com o remodelamento tecidual presente nas doenças reumáticas. Neste artigo, mostramos a importância do estudo do colágeno do tipo V no entendimento da etiologia da esclerodermia, no que se refere ao desencadeamento da auto-imunidade nesta enfermidade. Estudos em nosso laboratório demonstram que a sensibilização com colágeno do tipo V em coelhos pode resultar em um modelo animal de esclerodermia. Diante destes fatos, sugerimos que pesquisas neste campo podem ser de grande valia no desenvolvimento de novas condutas terapêuticas.The extracellular matrix (ECM proteins and their components have been widely studied in medical literature, as well its relationship with the tecidual remodeling present in the rheumatic disease. In this paper we show the importance of understanding the role of type V collagen as an important trigger of rheumatic autoimmune diseases. Studies in our laboratory demonstrate that type V collagen sensibilization in rabbits, could result in an animal scleroderma model. In this way we suggested that researches in this field can be worthy in development of new therapeutic procedures.

  11. Better Jobs, Brighter Futures, a Stronger Washington. Washington's Community and Technical Colleges

    Science.gov (United States)

    Washington State Board for Community and Technical Colleges, 2015

    2015-01-01

    The world is changing rapidly. With changes in technology, demographics, and workforce trends, Washington needs colleges to not only keep pace, but lead the way. Washington's 34 community and technical colleges answer that call. The community and technical colleges have proven uniquely positioned to adapt to, embrace, and ignite change. Community…

  12. Avaliação da preferência alimentar em modelo de poliartrite induzida por colágeno

    OpenAIRE

    Eduarda Correa Freitas

    2014-01-01

    Introdução: Artrite reumatoide é uma doença inflamatória crônica associada a alterações no metabolismo proteico e energético. Objetivos: Investigar a preferência alimentar durante o desenvolvimento da inflamação crônica em ratas com artrite induzida por colágeno. Métodos: Ratas Wistar fêmeas foram alocadas em dois grupos: controle (CO, n=10) e artrite induzida por colágeno (CIA; n=11) e expostas simultaneamente a quatro diferentes dietas (padrão, hipercalórica, hiperlipidica e hiperproteica)....

  13. A novel missense mutation of COL5A2 in a patient with Ehlers-Danlos syndrome.

    Science.gov (United States)

    Watanabe, Miki; Nakagawa, Ryuji; Naruto, Takuya; Kohmoto, Tomohiro; Suga, Ken-Ichi; Goji, Aya; Kagami, Shoji; Masuda, Kiyoshi; Imoto, Issei

    2016-01-01

    Ehlers-Danlos syndrome (EDS) is a group of inherited connective tissue disorders characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. For molecular diagnosis, targeted exome sequencing was performed on a 9-year-old male patient who was clinically suspected to have EDS. The patient presented with progressive kyphoscoliosis, joint hypermobility and hyperextensible skin without scars. Ultimately, classical EDS was diagnosed by identifying a novel, mono-allelic mutation in COL5A2 [NM_000393.3(COL5A2_v001):c.682G>A, p.Gly228Arg].

  14. A novel missense mutation of COL5A2 in a patient with Ehlers–Danlos syndrome

    Science.gov (United States)

    Watanabe, Miki; Nakagawa, Ryuji; Naruto, Takuya; Kohmoto, Tomohiro; Suga, Ken-ichi; Goji, Aya; Kagami, Shoji; Masuda, Kiyoshi; Imoto, Issei

    2016-01-01

    Ehlers–Danlos syndrome (EDS) is a group of inherited connective tissue disorders characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. For molecular diagnosis, targeted exome sequencing was performed on a 9-year-old male patient who was clinically suspected to have EDS. The patient presented with progressive kyphoscoliosis, joint hypermobility and hyperextensible skin without scars. Ultimately, classical EDS was diagnosed by identifying a novel, mono-allelic mutation in COL5A2 [NM_000393.3(COL5A2_v001):c.682G>A, p.Gly228Arg]. PMID:27656288

  15. An integrated DEA-COLS-SFA algorithm for optimization and policy making of electricity distribution units

    International Nuclear Information System (INIS)

    Azadeh, A.; Ghaderi, S.F.; Omrani, H.; Eivazy, H.

    2009-01-01

    This paper presents an integrated data envelopment analysis (DEA)-corrected ordinary least squares (COLS)-stochastic frontier analysis (SFA)-principal component analysis (PCA)-numerical taxonomy (NT) algorithm for performance assessment, optimization and policy making of electricity distribution units. Previous studies have generally used input-output DEA models for benchmarking and evaluation of electricity distribution units. However, this study proposes an integrated flexible approach to measure the rank and choose the best version of the DEA method for optimization and policy making purposes. It covers both static and dynamic aspects of information environment due to involvement of SFA which is finally compared with the best DEA model through the Spearman correlation technique. The integrated approach would yield in improved ranking and optimization of electricity distribution systems. To illustrate the usability and reliability of the proposed algorithm, 38 electricity distribution units in Iran have been considered, ranked and optimized by the proposed algorithm of this study.

  16. Asymptomatic parental mosaicism for osteogenesis imperfect associated with a new splice site mutation in COL1A2

    DEFF Research Database (Denmark)

    Frederiksen, Anja Lisbeth; Dunø, Morten; Johnsen, Iben Birgit Gade

    2016-01-01

    Recurrent lethal perinatal osteogenesis imperfecta may result from asymptomatic parental mosaicism. A previously unreported mutation in COL1A2 leads to recurrent cases of fetal osteogenesis imperfecta Sillence type IIA, which emphasizes the importance of clinical and genetic evaluation of mosaicism...

  17. Linkage analysis in a family with Stickler syndrome leads to the exclusion of the COL2A1 locus

    Energy Technology Data Exchange (ETDEWEB)

    Mottes, M.; Zolezzi, F.; Pignatti, P.F. [Univ. of Verona (Italy)

    1994-09-01

    Hereditary arthro-ophtalmopathy (AO) or Stickler Syndrome (MIM No. 10830) is a dominantly inherited disorder characterized by vitro-retinal degeneration and other connective tissue disturbances. Mutations in the COL2A1 gene, coding for type II collagen chains, have been described in a few patients. The wide spectrum of clinical manifestations is presumably due to genetic heterogeneity, since only about 50% of the Stickler families so far studied show cosegregation of the disease with the COL2A1 locus. We have investigated a large pedigree (19 individuals of whom 9 are affected) in which severe myopia with vitro-retinal degeneration consegregated with joint laxity, recurrent inguinal hernias, and degenerative changes of the hip and the knee. The 3{prime} end COL2A1 VNTR polymorphism was utilized for linkage analysis. In order to get the maximum informativity, we have analyzed the allelic microheterogeneity of this VNTR, due to the repeat sequence variation, by means of a single strand polymorphism. Mendelian inheritance of the different single strands was observed as expected. Discordance of segregation between the disease and the COL2A1 locus was thus established inequivocally in this family.

  18. Variability and distribution of COL1A2 (type I collagen) polymorphisms in the central-eastern Mediterranean Basin.

    Science.gov (United States)

    Scorrano, Gabriele; Lelli, Roberta; Martínez-Labarga, Cristina; Scano, Giuseppina; Contini, Irene; Hafez, Hani S; Rudan, Pavao; Rickards, Olga

    2016-01-01

    The most abundant of the collagen protein family, type I collagen is encoded by the COL1A2 gene. The COL1A2 restriction fragment length polymorphisms (RFLPs) EcoRI, RsaI and MspI in samples from several different central-eastern Mediterranean populations were analysed and found to be potentially informative anthropogenetic markers. The objective was to define the genetic variability of COL1A2 in the central-eastern Mediterranean and to shed light on its genetic distribution in human groups over a wide geographic area. PCR-RFLP analysis of EcoRI, RsaI and MspI polymorphisms of the COL1A2 gene was performed on oral swab and blood samples from 308 individuals from the central-eastern Mediterranean Basin. The genetic similarities among these groups and other populations described in the literature were investigated through correspondence analysis. Single-marker data and haplotype frequencies seemed to suggest a genetic homogeneity within the European populations, whereas a certain degree of differentiation was noted for the Egyptians and the Turks. The genetic variability in the central-eastern Mediterranean area is probably a result of the geographical barrier of the Mediterranean Sea, which separated European and African populations over time.

  19. Evaluation of a patient with classical Ehlers-Danlos syndrome due to a 9q34 duplication affecting COL5A1.

    Science.gov (United States)

    Kuroda, Yukiko; Ohashi, Ikuko; Naruto, Takuya; Ida, Kazumi; Enomoto, Yumi; Saito, Toshiyuki; Nagai, Jun-Ichi; Kurosawa, Kenji

    2018-03-09

    Ehlers-Danlos syndrome classical type is a connective tissue disorder characterized by skin hyperextensibility, atrophic scarring, and joint hypermobility. The condition typically results from mutations in COL5A1 or COL5A2 leading to the functional haploinsufficiency. Here, we report of a 24-year-old male with mild intellectual disability, dysmorphic features, and a phenotype consistent with Ehlers-Danlos syndrome classical type. A copy number variant-calling algorithm from panel sequencing data identified the deletions exons 2-11 and duplications of exons 12-67 within COL5A1. Array comparative genomic hybridization confirmed a 94 kb deletion at 9q34.3 involving exons 2-11 of COL5A1, and a 3.4 Mb duplication at 9q34.3 involving exons 12-67 of COL5A1. © 2018 Japanese Teratology Society.

  20. Washington Schools Learn from Value Engineering.

    Science.gov (United States)

    Doleae, Michael L.; Childs, Harvey C.

    1983-01-01

    Results of two value engineering studies have shown that a review early in the design process can help save costs in school construction, maintenance, operation, and replacement. The value engineering concepts and technical manual are being presented throughout the state of Washington. (MLF)

  1. Natural phenomena hazards, Hanford Site, Washington

    International Nuclear Information System (INIS)

    Conrads, T.J.

    1998-01-01

    This document presents the natural phenomena hazard loads for use in implementing DOE Order 5480.28, Natural Phenomena Hazards Mitigation, and supports development of double-shell tank systems specifications at the Hanford Site in south-central Washington State. The natural phenomena covered are seismic, flood, wind, volcanic ash, lightning, snow, temperature, solar radiation, suspended sediment, and relative humidity

  2. 40 CFR 81.348 - Washington.

    Science.gov (United States)

    2010-07-01

    ... 5/14/01 Attainment The City of Kent and a portion of the Green River valley bounded on the east and... Type Seattle-Tacoma Area: Seattle-Tacoma Urban Area (as defined by the Washington Department of Transportation urban area maps) King County (part) Attainment Pierce County (part) Attainment Snohomish County...

  3. Changing Housing Patterns in Metropolitan Washington

    Science.gov (United States)

    Grier, George; Grier, Eunice

    1975-01-01

    This testimony, before a public hearing of the New York City Commission on Human Rights in May 1974, summarizes extensive studies of changing minority residential patterns in metropolitan Washington and less extensive studies of other groups; the prospects for future desegregation and for using the growing economic potential of minority families…

  4. Washington Irving and the American Indian.

    Science.gov (United States)

    Littlefield, Daniel F., Jr.

    1979-01-01

    Some modern scholars feel that Washington Irving vacillated between romanticism and realism in his literary treatment of the American Indian. However, a study of all his works dealing with Indians, placed in context with his non-Indian works, reveals that his attitude towards Indians was intelligent and enlightened for his time. (CM)

  5. Doctors of Osteopathy Licensed in Washington.

    Science.gov (United States)

    Senters, Jo

    Based on information gathered by the Health Manpower Project through a survey cosponsored with the Washington Osteopathic Medical Association, this report begins with a statement of philosophy of osteopathic medicine and proceeds to comment on where such professional education is available. Remarks on the type of educational background of the…

  6. Washington Public Libraries Online: Collaborating in Cyberspace.

    Science.gov (United States)

    Wildin, Nancy

    1997-01-01

    Discussion of public libraries, the Internet, and the World Wide Web focuses on development of a Web site in Washington. Highlights include access to the Internet through online public access catalogs; partnerships between various types of libraries; hardware and software; HTML training; content design; graphics design; marketing; evaluation; and…

  7. Recidivism of Supermax Prisoners in Washington State

    Science.gov (United States)

    Lovell, David; Johnson, L. Clark; Cain, Kevin C.

    2007-01-01

    This study of recidivism among Washington supermax prisoners used a retrospective matched control design, matching supermax prisoners one-to-one with nonsupermax prisoners on mental illness status and up to eight recidivism predictors. Supermax prisoners committed new felonies at a higher rate than nonsupermax controls, but the difference was not…

  8. Timber resource statistics for eastern Washington, 1995.

    Science.gov (United States)

    Neil McKay; Patricia M. Bassett; Colin D. MacLean

    1995-01-01

    This report summarizes a 1990-91 timber resource inventory of Washington east of the crest of the Cascade Range. The inventory was conducted on all private and public lands except National Forests. Timber resource statistics from National Forest inventories also are presented. Detailed tables provide estimates of forest area, timber volume, growth, mortality, and...

  9. Timber resource statistics for western Washington.

    Science.gov (United States)

    Coffin D. MacLean; Patricia M. Bassett; Glenn. Yeary

    1992-01-01

    This report summarizes a 1988-90 timber resource inventory of 19 counties in western Washington: Clallam, Clark, Cowlitz, Grays Harbor, Island, Jefferson, King, Kitsap, Lewis, Mason, Pacific, Pierce, San Juan, Skagit, Skamania, Snohomish, Thurston, Wahkiakum, and Whatcom. Detailed tables of forest area, timber volume, growth, mortality, and harvest are presented.

  10. Washington (Wash) C. Winn: In Memoriam

    Centers for Disease Control (CDC) Podcasts

    2012-03-08

    Dr. Mike Miller and Dr. David Walker dicuss the career and life of noted clinical biologist, Dr. Washington C. Winn Jr.  Created: 3/8/2012 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 3/12/2012.

  11. Genetic Variations of COL4A1 Gene and Intracerebral Hemorrhage Outcome: A Cohort Study in a Chinese Han Population.

    Science.gov (United States)

    Xia, Chao; Lin, Sen; Yang, Jie; He, Sha; Li, Hao; Liu, Ming; You, Chao

    2018-05-01

    To investigate the relationship between single nucleotide polymorphisms or haplotypes of COL4A1 gene and the outcome of intracerebral hemorrhage (ICH). In our study, 181 patients with hypertensive ICH were enrolled and followed up at 3 and 6 months. Outcome data included any cause of death and disability. Genomic DNA was extracted by DNA extraction kit, and the 6 single nucleotide polymorphism genotyping of the COL4A1 gene was detected through MassARRAY Analyzer. Unphased 3.1.4 and SPSS 19.0 were used to analyze the association between alleles, genotypes, and haplotypes of the COL4A1 gene and the outcomes of ICH. Of the 181 patients with hypertensive ICH, 12 were lost in follow-up, which accounted for 6.6%. Our association analysis showed that the rs532625 AA genotype of the COL4A1 gene may increase risk of disability at 3 months; the rs532625 A allele and AA genotype were association factors of the risk of disability at 6 months; the rs532625 AA genotype was an association factor of the risk of death/disability at 6 months. After adjusting for gender, age, coma, and severe neurologic deficits, only the rs532625 AA genotype was independently associated with the risk of disability at 3 and 6 months and the risk of death/disability at 6 months. Our study found that the rs532625 AA genotype in the COL4A1 gene was independently associated with the risk of disability at 3 and 6 months and death/disability at 6 months in a Chinese Han population. These conclusions need to be verified in future studies with larger samples. Copyright © 2018 Elsevier Inc. All rights reserved.

  12. Targeted deletion of RANKL in M cell inducer cells by the Col6a1-Cre driver.

    Science.gov (United States)

    Nagashima, Kazuki; Sawa, Shinichiro; Nitta, Takeshi; Prados, Alejandro; Koliaraki, Vasiliki; Kollias, George; Nakashima, Tomoki; Takayanagi, Hiroshi

    2017-11-04

    The gut-associated lymphoid tissues (GALTs), including Peyer's patches (PPs), cryptopatches (CPs) and isolated lymphoid follicles (ILFs), establish a host-microbe symbiosis by the promotion of immune reactions against gut microbes. Microfold cell inducer (MCi) cells in GALTs are the recently identified mesenchymal cells that express the cytokine RANKL and initiate bacteria-specific immunoglobulin A (IgA) production via induction of microfold (M) cell differentiation. In the previous study, the Twist2-Cre driver was utilized for gene deletion in mesenchymal cells including MCi cells. In order to investigate MCi cells more extensively, it will be necessary to develop experimental tools in addition to the Twist2-Cre driver mice and characterize such drivers in specificity and efficiency. Here we show that M cell differentiation and IgA production are impaired in the targeted deletion of RANKL by the Col6a1-Cre driver. We compared Col6a1-Cre with Twist2-Cre in terms of the specificity for mesenchymal cells in GALTs. Col6a1-Cre CAG-CAT-EGFP mice exhibited EGFP expression in podoplanin + CD31 - cells including MCi cells, while Twist2-Cre mice were shown to target endothelial cells and podoplanin + CD31 - cells. Tnfsf11 fl/Δ Col6a1-Cre mice exhibited the absence of M cells and severe IgA reduction together with an alteration in gut microbial composition. Moreover, we analyzed germ free mice to test whether changes in the microbiota are the cause of M cell deficiency. M cell differentiation was normal in the CPs/ILFs of germ free mice, indicating that MCi cells induce M cells independently of microbial colonization. This study demonstrates that Col6a1-Cre driver mice are as useful as Twist2-Cre driver mice for functional analyses of GALT-resident mesenchymal cells, including MCi cells. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. Collagen Type I alpha1 (COL1A1 Gene Polymorphism and Bone Mineral Density in Postmenopausal Kazakh Women

    Directory of Open Access Journals (Sweden)

    Akbota Aitkulova

    2014-12-01

    Full Text Available Introduction: Single nucleotide polymorphism (SNP at the collagen type I alpha 1 gene (COL1A1 rs1800012 has been widely studied and has shown an association with bone mineral density (BMD and fractures. A minor allele TT of this SNP was found to be greatly overrepresented in individuals with fractures compared to controls, thus becoming a good predictor of  increased fracture risk. The aim of this investigation was to evaluate potential association between COL1A1 gene polymorphism and osteoporosis in Kazakh postmenopausal women.Methods: The study population included 103 postmenopausal women recruited from Pavlodar and Almaty clinics. BMD was measured using DEXA. Genomic DNA was extracted from peripheral venous blood of study participants with Wizard® Genomic DNA Purification Kit (Promega, USA. Detection of COL1A1 +1245G/T (Sp1 polymorphism was done by the TaqMan® SNP Genotyping Assay of real-time PCR.Results: Densitometry results revealed 36 osteoporotic, 42 osteopenic, and 25 normal postmenopausal women. Data analysis of 1245G>T polymorphism in COL1A1 gene in the group of women with osteopenia and osteoporosis revealed deviation from Hardy-Weinberg equilibrium. The mutant TT genotype was prevalent compared to the heterozygous genotype GT in both groups. Distributions were 83% GG, 3% GT, and 14% TT in the group with osteopenia and 80% GG, 6% GT, and 14% TT in the group with osteoporosis. The distribution of genotypes frequency in the group of normal postmenopausal women was 76% GG, 16% GT, and 8% TT.Conclusion: These results suggest that TT genotype of COL1A1 +1245G/T (Sp1 polymorphism is associated with risk of postmenopausal osteoporosis in Kazakh women. Further studies involving a larger number of women are needed to clarify the relationship of this polymorphism with risk of osteoporosis. 

  14. Drivers' use of marijuana in Washington state : traffic tech.

    Science.gov (United States)

    2016-08-01

    In July 2014, Washington State allowed legal sales of : recreational marijuana. Working with the Washington : Traffic Safety Commission, NHTSA assisted the State in : conducting a roadside study to examine the prevalence : of marijuana use before and...

  15. An assessment of interstate safety investment properties in Washington state.

    Science.gov (United States)

    2014-12-01

    The Washington State Department of Transportation (WSDOT) commissioned the current study, targeting the entire interstate : mainline network in Washington State, to provide strategic direction to multi-biennial investment interstate locations that of...

  16. Trends and determinants of cycling in the Washington, DC region.

    Science.gov (United States)

    2011-01-01

    This report analyzes cycling trends, policies, and commuting in the Washington, DC area. The analysis is divided into two parts. : Part 1 focuses on cycling trends and policies in Washington (DC), Alexandria (VA), Arlington County (VA), Fairfax Count...

  17. 76 FR 16323 - Irish Potatoes Grown in Washington; Continuance Referendum

    Science.gov (United States)

    2011-03-23

    ...; FV11-946-1 CR] Irish Potatoes Grown in Washington; Continuance Referendum AGENCY: Agricultural... conducted among eligible Washington potato growers to determine whether they favor continuance of the marketing order regulating the handling of Irish potatoes grown in Washington. DATES: The referendum will be...

  18. 76 FR 377 - Land Acquisitions; Cowlitz Indian Tribe of Washington

    Science.gov (United States)

    2011-01-04

    ... State of Washington by Auditor's File Nos. G 450664 and G 147358. Parcel II That portion of the... thereof acquired by the State of Washington by deed recorded under Auditor's File Nos. G 140380 and D... recorded under Auditor's File No. F 38759, records of Clark County, Washington, described as follows...

  19. 75 FR 52023 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2010-08-24

    ... DEPARTMENT OF THE INTERIOR National Park Service Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service... of the Thomas Burke Memorial Washington State Museum (Burke Museum), University of Washington...

  20. 75 FR 36672 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2010-06-28

    ... DEPARTMENT OF THE INTERIOR National Park Service Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service... of the Thomas Burke Memorial Washington State Museum (Burke Museum), University of Washington...

  1. ROCK inhibition stimulates SOX9/Smad3-dependent COL2A1 expression in inner meniscus cells.

    Science.gov (United States)

    Furumatsu, Takayuki; Maehara, Ami; Ozaki, Toshifumi

    2016-07-01

    Proper functioning of the meniscus depends on the composition and organization of its fibrocartilaginous extracellular matrix. We previously demonstrated that the avascular inner meniscus has a more chondrocytic phenotype compared with the outer meniscus. Inhibition of the Rho family GTPase ROCK, the major regulator of the actin cytoskeleton, stimulates the chondrogenic transcription factor Sry-type HMG box (SOX) 9-dependent α1(II) collagen (COL2A1) expression in inner meniscus cells. However, the crosstalk between ROCK inhibition, SOX9, and other transcription modulators on COL2A1 upregulation remains unclear in meniscus cells. The aim of this study was to investigate the role of SOX9-related transcriptional complex on COL2A1 expression under the inhibition of ROCK in human meniscus cells. Human inner and outer meniscus cells were prepared from macroscopically intact lateral menisci. Cells were cultured in the presence or absence of ROCK inhibitor (ROCKi, Y27632). Gene expression, collagen synthesis, and nuclear translocation of SOX9 and Smad2/3 were analyzed. Treatment of ROCKi increased the ratio of type I/II collagen double positive cells derived from the inner meniscus. In real-time PCR analyses, expression of SOX9 and COL2A1 genes was stimulated by ROCKi treatment in inner meniscus cells. ROCKi treatment also induced nuclear translocation of SOX9 and phosphorylated Smad2/3 in immunohistological analyses. Complex formation between SOX9 and Smad3 was increased by ROCKi treatment in inner meniscus cells. Chromatin immunoprecipitation analyses revealed that association between SOX9/Smad3 transcriptional complex with the COL2A1 enhancer region was increased by ROCKi treatment. This study demonstrated that ROCK inhibition stimulated SOX9/Smad3-dependent COL2A1 expression through the immediate nuclear translocation of Smad3 in inner meniscus cells. Our results suggest that ROCK inhibition can stimulates type II collagen synthesis through the cooperative activation

  2. An apple B-box protein, MdCOL11, is involved in UV-B- and temperature-induced anthocyanin biosynthesis.

    Science.gov (United States)

    Bai, Songling; Saito, Takanori; Honda, Chikako; Hatsuyama, Yoshimichi; Ito, Akiko; Moriguchi, Takaya

    2014-11-01

    Our studies showed that an apple B-box protein, MdCOL11, the homolog of AtBBX22, is involved in UV-B- and temperature-induced anthocyanin biosynthesis in apple peel. Anthocyanin is responsible for the red pigmentation in apple peel and a R2R3 MYB gene, MdMYBA/1/10, a homolog of MdMYBA, controls its accumulation. Arabidopsis PAP1 is under the control of a series of upstream factors involved in light signal transduction and photomorphogenesis, such as ELONGATED HYPOCOTYL 5 (HY5) and B-box family (BBX) proteins. In this study, we identified and characterized the homolog of Arabidopsis BBX22 in apple, designated as MdCOL11. Overexpression of MdCOL11 in Arabidopsis enhanced the accumulation of anthocyanin. In apples, MdCOL11 was differentially expressed in all tissues, with the highest expression in petals and the lowest expression in the xylem. Transcripts of MdCOL11 noticeably accumulated at the ripening stage, concomitant with increases in the expressions of anthocyanin biosynthesis-related genes. In an in vitro treatment experiment, MdCOL11 was upregulated in an ultra-violet (UV)-B- and temperature-dependent manner, together with the inductions of anthocyanin biosynthesis-related genes and anthocyanin accumulation in apple peel. Furthermore, a dual-luciferase assay indicated that (1) MdCOL11 regulated the expression of MdMYBA and (2) MdCOL11 was a target of MdHY5. Taken together, our results suggest that MdCOL11 is involved in MdHY5-mediated signal transduction and regulates anthocyanin accumulation in apple peel, which sheds new light on anthocyanin accumulation in apples.

  3. Linkage mapping of the gene for Type III collagen (COL3A1) to human chromosome 2q using a VNTR polymorphism

    Energy Technology Data Exchange (ETDEWEB)

    Tiller, G.E.; Polumbo, P.A.; Summar, M.L. (Vanderbilt Univ. Medical Center, Nashville, TN (United States))

    1994-03-15

    The gene for the [alpha]1(III) chain of type III collagen, COL3A1, has been previously mapped to human chromosome 2q24.3-q31 by in situ hybridization. Physical mapping by pulsed-field gel electrophoresis has demonstrated that COL3A1 lies within 35 kb of COL5A2. The authors genotyped the CEPH families at the COL3A2 locus using a pentanucleotide repeat polymorphism within intron 25. They demonstrated significant linkage to 18 anonymous markers as well as the gene for carbamyl phosphate synthetase (CPSI), which had been previously mapped to this region. No recombination was seen between COL3A1 and COL5A2 (Z = 9.93 at [theta] = 0) or D2S24 (Z = 10.55 at [theta] = 0). The locus order is (D2S32-D2S138-D2S148)-(D2S24-COL5A2-COL3A1)-(D2S118-D2S161), with odds of 1:2300 for the next most likely order. These relationships are consistent with the physical mapping of COL3A1 to the distal portion of 2q and place it proximal to CPSI by means of multipoint analysis. These linkage relationships should prove useful in further studies of Ehlers-Danlos syndrome type IV and carbamyl phosphate synthetase I deficiency and provide an additional framework for localizing other genes in this region. 13 refs., 2 figs., 1 tab.

  4. Low-temperature geothermal resources of Washington

    Energy Technology Data Exchange (ETDEWEB)

    Schuster, J.E. [Washington State Dept. of Natural Resources, Olympia, WA (United States). Div. of Geology and Earth Resources; Bloomquist, R.G. [Washington State Energy Office, Olympia, WA (United States)

    1994-06-01

    This report presents information on the location, physical characteristics, and water chemistry of low-temperature geothermal resources in Washington. The database includes 941 thermal (>20C or 68F) wells, 34 thermal springs, lakes, and fumaroles, and 238 chemical analyses. Most thermal springs occur in the Cascade Range, and many are associated with stratovolcanoes. In contrast, 97 percent of thermal wells are located in the Columbia Basin of southeastern Washington. Some 83.5 percent are located in Adams, Benton, Franklin, Grant, Walla Walla, and Yakima Counties. Yakima County, with 259 thermal wells, has the most. Thermal wells do not seem to owe their origin to local sources of heat, such as cooling magma in the Earth`s upper crust, but to moderate to deep circulation of ground water in extensive aquifers of the Columbia River Basalt Group and interflow sedimentary deposits, under the influence of a moderately elevated (41C/km) average geothermal gradient.

  5. Fuel management at Washington State Ferries

    International Nuclear Information System (INIS)

    Brodeur, P.; Olds, J.

    2008-01-01

    This presentation discussed Washington State Ferry (WSF) operations and provided details of a biodiesel research and demonstration project. Washington has the largest ferry system in the United States, with a total of 28 vessels that operate on 10 routes through 20 terminals. Routes vary by transit times, navigational challenges, and the proximity to population centres. WSF fuel and emissions management initiatives include exhaust emission studies, clean fuel initiatives, machinery upgrades, fuel conservation initiatives, and biodiesel testing. The organization is also using waste heat recovery and a positive restraint system. The WSF biodiesel pilot program was conducted using soy-derived fuels with a purifier disk stack. The program is in agreement with recent legislation requiring that 2 per cent of annual diesel fuel sales are from biodiesel fuels, and state legislation requiring that state agencies use a minimum of 20 per cent biodiesel blends in diesel-powered vessels and vehicles. Details of project partnerships were included. tabs., figs

  6. Osteoporosis and Related Genes: VDR, ESR And COL1A1

    Directory of Open Access Journals (Sweden)

    Sabriye Kocaturk Sel

    2011-08-01

    Full Text Available Osteoporosis is now considered as one of the major and growing health care problems around the world. Osteoporosis is the most prevalent metabolic bone disease among developed countries and it is defined as a systemic skeletal disease characterized by low bone mass and microarchitectural deterioration of bone tissue with a consequent increase in bone fragility. Bone is a highly metabolically active tissue in which the processes of osteoblastic bone formation and osteoclastic bone resorption are continuous throughout life. Coupling of osteoblast and osteoclast action ensures that a normal bone structure is maintained. A loss of bone homeostasis may result in a decrease in bone mass leading to osteoporosis or in a defect in the mineralization of bone. Numerous genetic, hormonal, nutritional and life-style factors contribute to the acquisition and maintenance of bone mass. Among them, genetic variations explain as much as 50-80% of the variance for bone mineral density (BMD in the population. Many genes that could be related to osteoporosis have been studied and of them all Vitamin D receptor (VDR, estrogen receptor alpha (ESRα and collagen 1 alpha 1 chain (COL1A1 genes have been the most focused on. [Archives Medical Review Journal 2011; 20(4.000: 246-269

  7. Cartografia e deficiência visual: experiências no Colégio Pedro II

    Directory of Open Access Journals (Sweden)

    Rafael Medeiros de Andrade

    2014-01-01

    Full Text Available O processo de construção de uma escola inclusiva perpassa um conjunto de esforços cognitivos e técnicos por parte da comunidade escolar que são necessários para a adequada educação e desenvolvimento do aluno. No que diz respeito ao ensino de Geografia para alunos deficientes visuais, fazem parte deste conjunto de medidas o domínio do sistema Braille, a confecção de materiais e mapas táteis, a gravação de textos em áudio e, entre outras, a realização de trabalhos de campo. O presente relato de experiência refere-se à um projeto de dedicação exclusiva, implementado no Colégio Pedro II de 2008 a 2010, cujo objetivo foi aprimorar o ensino de Geografia para os alunos deficientes visuais. Conclui-se que a adoção dessas medidas representa o início de um longo trabalho de toda comunidade escolar que não pode ser resumido à existência de alguns materiais táteis. Deve, por outro lado, envolver um processo de construção e adequação instrumental, acompanhado da capacitação profissional e da sensibilização da comunidade escolar.

  8. Xarxa interclase: un projecte col·laboratiu mitjançant tecnologies digitals

    Directory of Open Access Journals (Sweden)

    Françoise Olmo Cazevieille

    2015-12-01

    Full Text Available Focalitzem aquest treball en l'aportació de les tecnologies de la informació i la comunicació (TIC a la pràctica i el desenvolupament d'una llengua estrangera i en els avantatges que ofereixen per a la creació d'activitats basades en la col·laboració i la interacció. Sense aquestes, tant els treballs en equip amb nadius com la cerca d'informació sobre temes especialitzats en la llengua estudiada serien complexos de dur a terme. En aquest article presentem el procediment seguit en un projecte didàctic, Xarxa interclasse, entre dues assignatures basades en l'aprenentatge de llengües estrangeres per a fins específics en un mateix campus universitari.?Es pretén, d'una banda, acostar l'educació superior a les exigències de la societat actual i, d'altra banda, aconseguir una docència motivadora i una participació activa dels discents on la tecnologia s'integra com a element pedagògic.

  9. Unique mutation portraits and frequent COL2A1 gene alteration in chondrosarcoma

    Science.gov (United States)

    Totoki, Yasushi; Yoshida, Akihiko; Hosoda, Fumie; Nakamura, Hiromi; Hama, Natsuko; Ogura, Koichi; Yoshida, Aki; Fujiwara, Tomohiro; Arai, Yasuhito; Toguchida, Junya; Tsuda, Hitoshi; Miyano, Satoru; Kawai, Akira

    2014-01-01

    Chondrosarcoma is the second most frequent malignant bone tumor. However, the etiological background of chondrosarcomagenesis remains largely unknown, along with details on molecular alterations and potential therapeutic targets. Massively parallel paired-end sequencing of whole genomes of 10 primary chondrosarcomas revealed that the process of accumulation of somatic mutations is homogeneous irrespective of the pathological subtype or the presence of IDH1 mutations, is unique among a range of cancer types, and shares significant commonalities with that of prostate cancer. Clusters of structural alterations localized within a single chromosome were observed in four cases. Combined with targeted resequencing of additional cartilaginous tumor cohorts, we identified somatic alterations of the COL2A1 gene, which encodes an essential extracellular matrix protein in chondroskeletal development, in 19.3% of chondrosarcoma and 31.7% of enchondroma cases. Epigenetic regulators (IDH1 and YEATS2) and an activin/BMP signal component (ACVR2A) were recurrently altered. Furthermore, a novel FN1-ACVR2A fusion transcript was observed in both chondrosarcoma and osteochondromatosis cases. With the characteristic accumulative process of somatic changes as a background, molecular defects in chondrogenesis and aberrant epigenetic control are primarily causative of both benign and malignant cartilaginous tumors. PMID:25024164

  10. Monomer-dimer control of the ColE1 P(cer) promoter.

    Science.gov (United States)

    Chatwin, H M; Summers, D K

    2001-11-01

    XerCD-mediated recombination at cer converts multimers of plasmid ColE1 to monomers, maximizing the number of independently segregating molecules and minimizing the frequency of plasmid loss. In addition to XerCD, recombination requires the accessory factors ArgR and PepA. The promoter P(cer), located centrally within cer, is also required for stable plasmid maintenance. P(cer) is active in plasmid multimers and directs transcription of a short RNA, Rcd, which appears to inhibit cell division. It has been proposed that Rcd is part of a checkpoint which ensures that multimer resolution is complete before the cell divides. This study has shown that ArgR does not act as a transcriptional repressor of P(cer) in plasmid monomers. P(cer) is unusual in that the -35 and -10 hexamers are separated by only 15 bp and this study has demonstrated that increasing this to a more conventional spacing results in elevated activity. An increase to 17 bp resulted in a 10- to 20-fold increase in activity, while smaller effects were seen when the spacer was increased to 16 bp or 18 bp. These observations are consistent with the hypothesis that P(cer) activation involves realignment of the -35 and -10 sequences within a recombinational synaptic complex. This predicts that a 17 bp spacer promoter derivative should be down-regulated by plasmid multimerization, and this is confirmed experimentally.

  11. Osteogenesis imperfecta with ectopic mineralizations in dentin and cementum and a COL1A2 mutation.

    Science.gov (United States)

    Kantaputra, Piranit Nik; Sirirungruangsarn, Yuddhasert; Intachai, Worrachet; Ngamphiw, Chumpol; Tongsima, Sissades; Dejkhamron, Prapai

    2018-04-10

    We report a Thai father (patient 1) and his daughter (patient 2) affected with osteogenesis imperfecta type IV and dentinogenesis imperfecta. Both were heterozygous for the c.1451G>A (p.Gly484Glu) mutation in COL1A2. The father, a Thai boxer, had very mild osteogenesis imperfecta with no history of low-trauma bone fractures. Scanning electron micrography of the primary teeth with DI of the patient 2, and the primary teeth with DI of another OI patient with OI showed newly recognized dental manifestations of teeth with DI. Normal dentin and cementum might have small areas of ectopic mineralizations. Teeth affected with DI have well-organized ectopic mineralizations in dentin and cementum. The "French-fries-appearance" of the crystals at the cemento-dentinal junction and abnormal cementum have never been reported to be associated with dentinogenesis imperfecta, either isolated or osteogenesis imperfecta-associated. Our study shows for the first time that abnormal collagen fibers can lead to ectopic mineralization in dentin and cementum and abnormal cementum can be a part of osteogenesis imperfecta.

  12. Snag Dynamics in Western Oregon and Washington

    OpenAIRE

    Ohmann, Janet L

    2002-01-01

    To achieve desired amounts and characteristics of snags and down wood, managers require analytical tools for projecting changes in dead wood over time, and for comparing those changes to management objectives such as providing dead wood for wildlife and ecosystem processes. The following information on rates of snag recruitment, decay, and fall across forests of western Oregon and Washington may be useful in planning for future levels of dead wood. Eventually the information will be incorpora...

  13. Different NaCl-Induced Calcium Signatures in the Arabidopsis thaliana Ecotypes Col-0 and C24

    KAUST Repository

    Schmö ckel, Sandra M.; Garcia, Alexandre F.; Berger, Bettina; Tester, Mark A.; Webb, Alex A. R.; Roy, Stuart J.

    2015-01-01

    A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.

  14. Different NaCl-Induced Calcium Signatures in the Arabidopsis thaliana Ecotypes Col-0 and C24

    KAUST Repository

    Schmöckel, Sandra M.

    2015-02-27

    A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.

  15. 77 FR 64718 - Safety Zone; Steam Ship Col. James M. Schoonmaker Relocation Project, Maumee River, Toledo, OH

    Science.gov (United States)

    2012-10-23

    ...The Coast Guard is establishing a temporary Safety Zone on the waters of Maumee River, Toledo, Ohio, in support of the relocation of S/S Col. James M. Schoonmaker (hereinafter referred to as `the Schoonmaker'). This temporary safety zone will encompass all waters of the Maumee River within a 100 yard radius of the Schoonmaker as it makes its transit from International Park to Skyway Marina. This temporary Safety Zone is necessary to protect persons operating around the area.

  16. Projecting Politics: The Grapes of Wrath Les Raisins de la colère à l’écran

    Directory of Open Access Journals (Sweden)

    Stephen J. Whitfield

    2009-07-01

    Full Text Available Publié en 1939 et adapté à l’écran l’année suivante, Les Raisins de la colère, roman de John Steinbeck, eut un impact immédiat et extraordinaire pour le portrait sans concession qu’il dressait d’un capitalisme américain qui semblait avoir abandonné sa progéniture la plus loyale, la plus assidue et la plus courageuse, celle qui labourait la terre en plein coeur de la république. Écrit à la toute fin de la « Décennie rouge », mais juste avant que les États-Unis entrent dans la Seconde Guerre mondiale, Les Raisins de la colère exprimaient, d’une certaine manière, la colère accumulée contre un système économique qui avait trahi le prolétariat rural. Le roman, tout comme le film de John Ford (1940, furent compris par le grand public de l’époque, et bien des années plus tard, comme une protestation clairement gauchiste, voire radicale, contre une telle injustice. Mais cet essai montre combien les idées politiques de ces œuvres jumelées sont en réalité difficiles à saisir et combien il est hasardeux de définir, quel que soit l’effort rétrospectif, la critique que Steinbeck et Ford ont proposée dans leur version des Raisins de la colère, une œuvre inoubliable, déchirante mais ambiguë sur le plan politique.

  17. Support of positive association in family-based genetic analysis between COL27A1 and Tourette syndrome.

    Science.gov (United States)

    Liu, Shiguo; Yu, Xiaoxia; Xu, Quanchen; Cui, Jiajia; Yi, Mingji; Zhang, Xinhua; Ge, Yinlin; Ma, Xu

    2015-08-03

    Recently, a genome-wide association study has indicated associations between single nucleotide polymorphisms in the Collagen Type XXVII Alpha 1 gene (COL27A1) and Tourette syndrome in several ethnic populations. To clarify the global relevance of the previously identified SNPs in the development of Tourette syndrome, the associations between polymorphisms in COL27A1 and Tourette syndrome were assessed in Chinese trios. PCR-directed sequencing was used to evaluate the genetic contributions of three SNPs in COL27A1(rs4979356, rs4979357 and rs7868992) using haplotype relative risk (HRR) and transmission disequilibrium tests (TDT) with a total of 260 Tourette syndrome trios. The family-based association was significant between Tourette syndrome and rs4979356 (TDT: χ2 = 4.804, P = 0.033; HRR = 1.75, P = 0.002; HHRR = 1.32, P = 0.027), and transmission disequilibrium was suspected for rs4979357 (TDT: χ2 = 3.969, P = 0.053; HRR = 1.84, P = 0.001; HHRR = 1.29, P = 0.044). No statistically significant allele transfer was found for rs7868992 (TDT: χ2 = 2.177, P = 0.158). Although the TDT results did not remain significant after applying the conservative Bonferroni correction (p = 0.005), the significant positive HRR analysis confirmed the possibility of showing transmission disequilibrium, which provides evidence for an involvement of COL27A1in the development of TS. However, these results need to be verified with larger datasets from different populations.

  18. Projecting Politics: The Grapes of Wrath Les Raisins de la colère : Une critique politique

    Directory of Open Access Journals (Sweden)

    Stephen J. Whitfield

    2008-01-01

    Full Text Available Publié en 1939 et adapté à l’écran l’année suivante, Les Raisins de la colère, roman de John Steinbeck, eut un impact immédiat et extraordinaire pour le portrait sans concession qu’il dressait d’un capitalisme américain qui semblait avoir abandonné sa progéniture la plus loyale, la plus assidue et la plus courageuse, celle qui labourait la terre en plein cœur de la république. Écrit à la toute fin de la « Décennie rouge », mais juste avant que les États-Unis entrent dans la Seconde Guerre mondiale, Les Raisins de la colère exprimaient, d’une certaine manière, la colère accumulée contre un système économique qui avait trahi le prolétariat rural. Le roman tout comme le film de John Ford (1940 furent compris par le grand public de l’époque, et bien des années plus tard, comme une protestation clairement gauchiste, voire radicale, contre une telle injustice. Mais cet essai montre combien les idées politiques de ces œuvres jumelées sont en réalité difficiles à saisir et combien il est hasardeux de définir, quel que soit l’effort rétrospectif, la critique que Steinbeck et Ford ont proposée dans leur version des Raisins de la colère, une œuvre inoubliable, déchirante mais ambiguë sur le plan politique.

  19. Sigma Theta Tau, DAaL/Texas Nurse Association, 2017 South Texas Nurse Imagemaker, Lt Col Jacqueline Killian

    Science.gov (United States)

    2018-04-28

    ENDORSEMENT (59 MDW/SGVU Use Only) 43. REVIEWER SIGNATURE 32. DATE 38. DATE 44. DATE April 11 , 20 17 45. DATE RECEIVED 1 46. SENIOR AUTHOR NOTIFIED BY...EBP mentors to facilitate the growing number of EBP projects underway in our organization. 1 Lt Col Killian serves as research consultant to chief...for helping others. The first project is focused on enhancing resil ience in our military families--the " Mentors Offering Maternal Support (M-0-M

  20. Increased Frequency of ColV Plasmids and Mannose-Resistant Hemagglutinating Activity in an Escherichia coli K1 Population

    OpenAIRE

    1984-01-01

    The expression of traits linked to pathogenicity was studied in a population of Escherichia coli K1 strains. It was found that E. coli K1 strains isolated from extraintestinal infection harbor the ColV plasmid and express mannose-resistant hemagglutinating activity type VI with a high frequency. The presence of these properties may play a role in the ability of some E. coli K1 serogroups to invade.

  1. CNV analysis in Tourette syndrome implicates large genomic rearrangements in COL8A1 and NRXN1.

    Directory of Open Access Journals (Sweden)

    Abhishek Nag

    Full Text Available Tourette syndrome (TS is a neuropsychiatric disorder with a strong genetic component. However, the genetic architecture of TS remains uncertain. Copy number variation (CNV has been shown to contribute to the genetic make-up of several neurodevelopmental conditions, including schizophrenia and autism. Here we describe CNV calls using SNP chip genotype data from an initial sample of 210 TS cases and 285 controls ascertained in two Latin American populations. After extensive quality control, we found that cases (N = 179 have a significant excess (P = 0.006 of large CNV (>500 kb calls compared to controls (N = 234. Amongst 24 large CNVs seen only in the cases, we observed four duplications of the COL8A1 gene region. We also found two cases with ∼400 kb deletions involving NRXN1, a gene previously implicated in neurodevelopmental disorders, including TS. Follow-up using multiplex ligation-dependent probe amplification (and including 53 more TS cases validated the CNV calls and identified additional patients with rearrangements in COL8A1 and NRXN1, but none in controls. Examination of available parents indicates that two out of three NRXN1 deletions detected in the TS cases are de-novo mutations. Our results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions.

  2. Genetic and epigenetic factors at COL2A1 and ABCA4 influence clinical outcome in congenital toxoplasmosis.

    Directory of Open Access Journals (Sweden)

    Sarra E Jamieson

    2008-06-01

    Full Text Available Primary Toxoplasma gondii infection during pregnancy can be transmitted to the fetus. At birth, infected infants may have intracranial calcification, hydrocephalus, and retinochoroiditis, and new ocular lesions can occur at any age after birth. Not all children who acquire infection in utero develop these clinical signs of disease. Whilst severity of disease is influenced by trimester in which infection is acquired by the mother, other factors including genetic predisposition may contribute.In 457 mother-child pairs from Europe, and 149 child/parent trios from North America, we show that ocular and brain disease in congenital toxoplasmosis associate with polymorphisms in ABCA4 encoding ATP-binding cassette transporter, subfamily A, member 4. Polymorphisms at COL2A1 encoding type II collagen associate only with ocular disease. Both loci showed unusual inheritance patterns for the disease allele when comparing outcomes in heterozygous affected children with outcomes in affected children of heterozygous mothers. Modeling suggested either an effect of mother's genotype, or parent-of-origin effects. Experimental studies showed that both ABCA4 and COL2A1 show isoform-specific epigenetic modifications consistent with imprinting.These associations between clinical outcomes of congenital toxoplasmosis and polymorphisms at ABCA4 and COL2A1 provide novel insight into the molecular pathways that can be affected by congenital infection with this parasite.

  3. CNV analysis in Tourette syndrome implicates large genomic rearrangements in COL8A1 and NRXN1.

    Science.gov (United States)

    Nag, Abhishek; Bochukova, Elena G; Kremeyer, Barbara; Campbell, Desmond D; Muller, Heike; Valencia-Duarte, Ana V; Cardona, Julio; Rivas, Isabel C; Mesa, Sandra C; Cuartas, Mauricio; Garcia, Jharley; Bedoya, Gabriel; Cornejo, William; Herrera, Luis D; Romero, Roxana; Fournier, Eduardo; Reus, Victor I; Lowe, Thomas L; Farooqi, I Sadaf; Mathews, Carol A; McGrath, Lauren M; Yu, Dongmei; Cook, Ed; Wang, Kai; Scharf, Jeremiah M; Pauls, David L; Freimer, Nelson B; Plagnol, Vincent; Ruiz-Linares, Andrés

    2013-01-01

    Tourette syndrome (TS) is a neuropsychiatric disorder with a strong genetic component. However, the genetic architecture of TS remains uncertain. Copy number variation (CNV) has been shown to contribute to the genetic make-up of several neurodevelopmental conditions, including schizophrenia and autism. Here we describe CNV calls using SNP chip genotype data from an initial sample of 210 TS cases and 285 controls ascertained in two Latin American populations. After extensive quality control, we found that cases (N = 179) have a significant excess (P = 0.006) of large CNV (>500 kb) calls compared to controls (N = 234). Amongst 24 large CNVs seen only in the cases, we observed four duplications of the COL8A1 gene region. We also found two cases with ∼400 kb deletions involving NRXN1, a gene previously implicated in neurodevelopmental disorders, including TS. Follow-up using multiplex ligation-dependent probe amplification (and including 53 more TS cases) validated the CNV calls and identified additional patients with rearrangements in COL8A1 and NRXN1, but none in controls. Examination of available parents indicates that two out of three NRXN1 deletions detected in the TS cases are de-novo mutations. Our results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions.

  4. A experiência com arte na Colônia Juliano Moreira na década de 1950

    Directory of Open Access Journals (Sweden)

    João Henrique Queiroz de Araújo

    Full Text Available Resumo Os trabalhos de Osório César e de Nise da Silveira costumam se destacar quando se investiga a relação entre expressão artística e saberes psi no Brasil em torno da década de 1950. Porém, outras experiências ocorreram no mesmo período, como as atividades de pintura realizadas na Seção de Praxiterapia da Colônia Juliano Moreira, em Jacarepaguá, no município do Rio de Janeiro. A ausência de pesquisas sobre essa experiência suscitou uma busca por documentos que culminou na análise dos números do Boletim da Colônia Juliano Moreira e demais fontes. Os resultados apontam características peculiares da experiência artística na colônia, como o incentivo à cópia e reprodução de fotografias como método terapêutico, o que a diferencia das outras experiências mencionadas.

  5. Expressão do colágeno I em carcinomas epidermóides da cavidade oral

    Directory of Open Access Journals (Sweden)

    Martins Gabriela Botelho

    2003-01-01

    Full Text Available A matriz extracelular no carcinoma epidermóide da cavidade oral sofre modificações qualitativas e quantitativas durante a sua progressão. Estas alterações parecem possibilitar os processos de invasão e metástase que caracterizam as neoplasias malignas e são mais evidentes na interface tumor-hospedeiro. No nosso estudo, 23 biópsias incisionais de carcinomas epidermóides de diversas localizações da cavidade oral foram classificadas segundo o sistema de graduação histológica preconizado por Anneroth et al.¹ (1987. Foram utilizadas as colorações pela H. E. e a do tipo picrosirius, específica para colágeno. A expressão imuno-histoquímica do colágeno tipo I foi analisada em lesões com diferentes escores histológicos de malignidade. Pôde-se concluir que o colágeno apresenta diferentes padrões morfológicos, histoquímicos e imuno-histoquímicos em lesões com altos e baixos escores de malignidade.

  6. Genetic and Epigenetic Factors at COL2A1 and ABCA4 Influence Clinical Outcome in Congenital Toxoplasmosis

    Science.gov (United States)

    Jamieson, Sarra E.; de Roubaix, Lee-Anne; Cortina-Borja, Mario; Tan, Hooi Kuan; Mui, Ernest J.; Cordell, Heather J.; Kirisits, Michael J.; Miller, E. Nancy; Peacock, Christopher S.; Hargrave, Aubrey C.; Coyne, Jessica J.; Boyer, Kenneth; Bessieres, Marie-Hélène; Buffolano, Wilma; Ferret, Nicole; Franck, Jacqueline; Kieffer, François; Meier, Paul; Nowakowska, Dorota E.; Paul, Malgorzata; Peyron, François; Stray-Pedersen, Babill; Prusa, Andrea-Romana; Thulliez, Philippe; Wallon, Martine; Petersen, Eskild; McLeod, Rima; Gilbert, Ruth E.; Blackwell, Jenefer M.

    2008-01-01

    Background Primary Toxoplasma gondii infection during pregnancy can be transmitted to the fetus. At birth, infected infants may have intracranial calcification, hydrocephalus, and retinochoroiditis, and new ocular lesions can occur at any age after birth. Not all children who acquire infection in utero develop these clinical signs of disease. Whilst severity of disease is influenced by trimester in which infection is acquired by the mother, other factors including genetic predisposition may contribute. Methods and Findings In 457 mother-child pairs from Europe, and 149 child/parent trios from North America, we show that ocular and brain disease in congenital toxoplasmosis associate with polymorphisms in ABCA4 encoding ATP-binding cassette transporter, subfamily A, member 4. Polymorphisms at COL2A1 encoding type II collagen associate only with ocular disease. Both loci showed unusual inheritance patterns for the disease allele when comparing outcomes in heterozygous affected children with outcomes in affected children of heterozygous mothers. Modeling suggested either an effect of mother's genotype, or parent-of-origin effects. Experimental studies showed that both ABCA4 and COL2A1 show isoform-specific epigenetic modifications consistent with imprinting. Conclusions These associations between clinical outcomes of congenital toxoplasmosis and polymorphisms at ABCA4 and COL2A1 provide novel insight into the molecular pathways that can be affected by congenital infection with this parasite. PMID:18523590

  7. Extensive Analysis of GmFTL and GmCOL Expression in Northern Soybean Cultivars in Field Conditions.

    Science.gov (United States)

    Guo, Guangyu; Xu, Kun; Zhang, Xiaomei; Zhu, Jinlong; Lu, Mingyang; Chen, Fulu; Liu, Linpo; Xi, Zhang-Ying; Bachmair, Andreas; Chen, Qingshan; Fu, Yong-Fu

    2015-01-01

    The FLOWERING LOCUS T (FT) gene is a highly conserved florigen gene among flowering plants. Soybean genome encodes six homologs of FT, which display flowering activity in Arabidopsis thaliana. However, their contributions to flowering time in different soybean cultivars, especially in field conditions, are unclear. We employed six soybean cultivars with different maturities to extensively investigate expression patterns of GmFTLs (Glycine max FT-like) and GmCOLs (Glycine max CO-like) in the field conditions. The results show that GmFTL3 is an FT homolog with the highest transcript abundance in soybean, but other GmFTLs may also contribute to flower induction with different extents, because they have more or less similar expression patterns in developmental-, leaf-, and circadian-specific modes. And four GmCOL genes (GmCOL1/2/5/13) may confer to the expression of GmFTL genes. Artificial manipulation of GmFTL expression by transgenic strategy (overexpression and RNAi) results in a distinct change in soybean flowering time, indicating that GmFTLs not only impact on the control of flowering time, but have potential applications in the manipulation of photoperiodic adaptation in soybean. Additionally, transgenic plants show that GmFTLs play a role in formation of the first flowers and in vegetative growth.

  8. Common variants in the COL4A4 gene confer susceptibility to lattice degeneration of the retina.

    Science.gov (United States)

    Meguro, Akira; Ideta, Hidenao; Ota, Masao; Ito, Norihiko; Ideta, Ryuichi; Yonemoto, Junichi; Takeuchi, Masaki; Uemoto, Riyo; Nishide, Tadayuki; Iijima, Yasuhito; Kawagoe, Tatsukata; Okada, Eiichi; Shiota, Tomoko; Hagihara, Yuta; Oka, Akira; Inoko, Hidetoshi; Mizuki, Nobuhisa

    2012-01-01

    Lattice degeneration of the retina is a vitreoretinal disorder characterized by a visible fundus lesion predisposing the patient to retinal tears and detachment. The etiology of this degeneration is still uncertain, but it is likely that both genetic and environmental factors play important roles in its development. To identify genetic susceptibility regions for lattice degeneration of the retina, we performed a genome-wide association study (GWAS) using a dense panel of 23,465 microsatellite markers covering the entire human genome. This GWAS in a Japanese cohort (294 patients with lattice degeneration and 294 controls) led to the identification of one microsatellite locus, D2S0276i, in the collagen type IV alpha 4 (COL4A4) gene on chromosome 2q36.3. To validate the significance of this observation, we evaluated the D2S0276i region in the GWAS cohort and in an independent Japanese cohort (280 patients and 314 controls) using D2S0276i and 47 single nucleotide polymorphisms covering the region. The strong associations were observed in D2S0276i and rs7558081 in the COL4A4 gene (Pc = 5.8 × 10(-6), OR = 0.63 and Pc = 1.0 × 10(-5), OR = 0.69 in a total of 574 patients and 608 controls, respectively). Our findings suggest that variants in the COL4A4 gene may contribute to the development of lattice degeneration of the retina.

  9. Common variants in the COL4A4 gene confer susceptibility to lattice degeneration of the retina.

    Directory of Open Access Journals (Sweden)

    Akira Meguro

    Full Text Available Lattice degeneration of the retina is a vitreoretinal disorder characterized by a visible fundus lesion predisposing the patient to retinal tears and detachment. The etiology of this degeneration is still uncertain, but it is likely that both genetic and environmental factors play important roles in its development. To identify genetic susceptibility regions for lattice degeneration of the retina, we performed a genome-wide association study (GWAS using a dense panel of 23,465 microsatellite markers covering the entire human genome. This GWAS in a Japanese cohort (294 patients with lattice degeneration and 294 controls led to the identification of one microsatellite locus, D2S0276i, in the collagen type IV alpha 4 (COL4A4 gene on chromosome 2q36.3. To validate the significance of this observation, we evaluated the D2S0276i region in the GWAS cohort and in an independent Japanese cohort (280 patients and 314 controls using D2S0276i and 47 single nucleotide polymorphisms covering the region. The strong associations were observed in D2S0276i and rs7558081 in the COL4A4 gene (Pc = 5.8 × 10(-6, OR = 0.63 and Pc = 1.0 × 10(-5, OR = 0.69 in a total of 574 patients and 608 controls, respectively. Our findings suggest that variants in the COL4A4 gene may contribute to the development of lattice degeneration of the retina.

  10. Sexual dimorphism and inter-individual variation in the rove beetle, Creophilus maxillosus L. (Col: Staphylinidae

    Directory of Open Access Journals (Sweden)

    Mohammad Shahbaz

    2017-12-01

    Full Text Available Sexual selection is expected to drive phenotypic differences between conspecific male and females, a widespread phenomenon known as sexual dimorphism. At the same time, individuals may exhibit some degree of intra-sexual variation. We examined the sexual dimorphism and inter-individual variation in different body parts of Creophilus maxillosus L. (Col: Staphylinidae, a cosmopolitan rove beetle commonly found on carrion. Male C. maxillosus had significantly wider head and pronotum, longer mandibles, and more distant eyes than females. The head width was positively correlated to mandible length, which may reflect stronger adductor muscles and higher bite force in larger individuals. The allometry of traits can be examined by plotting the logarithms of that specific trait against the logarithm of body size and determining the slope (b of the regression line. Isometry occurs when b=1, i.e. the ratio of given traits to body size remains constant across individuals. Negative allometry occurs when b1, so that larger individuals have disproportionately larger traits. A positive allometry was found in head width (b=1.32, mandible length (b=2.28, and ocular distance (b=1.49 of males. Our results show that, particularly head size, mandible length and ocular distance are probably under sexual selection in males, while traits such as eye size are isometric to body size. The potential role of these traits in male-male combat as well as female attractiveness has been frequently documented in different insect taxa. The striking similarities in patterns of sexual dimorphism among independently evolved insects indicate that common evolutionary force(s are probably at work.

  11. The BiSciCol Triplifier: bringing biodiversity data to the Semantic Web.

    Science.gov (United States)

    Stucky, Brian J; Deck, John; Conlin, Tom; Ziemba, Lukasz; Cellinese, Nico; Guralnick, Robert

    2014-07-29

    Recent years have brought great progress in efforts to digitize the world's biodiversity data, but integrating data from many different providers, and across research domains, remains challenging. Semantic Web technologies have been widely recognized by biodiversity scientists for their potential to help solve this problem, yet these technologies have so far seen little use for biodiversity data. Such slow uptake has been due, in part, to the relative complexity of Semantic Web technologies along with a lack of domain-specific software tools to help non-experts publish their data to the Semantic Web. The BiSciCol Triplifier is new software that greatly simplifies the process of converting biodiversity data in standard, tabular formats, such as Darwin Core-Archives, into Semantic Web-ready Resource Description Framework (RDF) representations. The Triplifier uses a vocabulary based on the popular Darwin Core standard, includes both Web-based and command-line interfaces, and is fully open-source software. Unlike most other RDF conversion tools, the Triplifier does not require detailed familiarity with core Semantic Web technologies, and it is tailored to a widely popular biodiversity data format and vocabulary standard. As a result, the Triplifier can often fully automate the conversion of biodiversity data to RDF, thereby making the Semantic Web much more accessible to biodiversity scientists who might otherwise have relatively little knowledge of Semantic Web technologies. Easy availability of biodiversity data as RDF will allow researchers to combine data from disparate sources and analyze them with powerful linked data querying tools. However, before software like the Triplifier, and Semantic Web technologies in general, can reach their full potential for biodiversity science, the biodiversity informatics community must address several critical challenges, such as the widespread failure to use robust, globally unique identifiers for biodiversity data.

  12. Tissue-specific mosaicism for a lethal osteogenesis imperfecta COL1A1 mutation causes mild OI/EDS overlap syndrome.

    Science.gov (United States)

    Symoens, Sofie; Steyaert, Wouter; Demuynck, Lynn; De Paepe, Anne; Diderich, Karin E M; Malfait, Fransiska; Coucke, Paul J

    2017-04-01

    Type I collagen is the predominant protein of connective tissues such as skin and bone. Mutations in the type I collagen genes (COL1A1 and COL1A2) mainly cause osteogenesis imperfecta (OI). We describe a patient with clinical signs of Ehlers-Danlos syndrome (EDS), including fragile skin, easy bruising, recurrent luxations, and fractures resembling mild OI. Biochemical collagen analysis of the patients' dermal fibroblasts showed faint overmodification of the type I collagen bands, a finding specific for structural defects in type I collagen. Bidirectional Sanger sequencing detected an in-frame deletion in exon 44 of COL1A1 (c.3150_3158del), resulting in the deletion of three amino acids (p.Ala1053_Gly1055del) in the collagen triple helix. This COL1A1 mutation was hitherto identified in four probands with lethal OI, and never in EDS patients. As the peaks on the electropherogram corresponding to the mutant allele were decreased in intensity, we performed next generation sequencing of COL1A1 to study mosaicism in skin and blood. While approximately 9% of the reads originating from fibroblast gDNA harbored the COL1A1 deletion, the deletion was not detected in gDNA from blood. Most likely, the mild clinical symptoms observed in our patient can be explained by the mosaic state of the mutation. © 2017 Wiley Periodicals, Inc.

  13. Identification of a novel COL1A1 frameshift mutation, c.700delG, in a Chinese osteogenesis imperfecta family

    Science.gov (United States)

    Wang, Xiran; Pei, Yu; Dou, Jingtao; Lu, Juming; Li, Jian; Lv, Zhaohui

    2015-01-01

    Osteogenesis imperfecta (OI) is a family of genetic disorders associated with bone loss and fragility. Mutations associated with OI have been found in genes encoding the type I collagen chains. People with OI type I often produce insufficient α1-chain type I collagen because of frameshift, nonsense, or splice site mutations in COL1A1 or COL1A2. This report is of a Chinese daughter and mother who had both experienced two bone fractures. Because skeletal fragility is predominantly inherited, we focused on identifying mutations in COL1A1 and COL1A2 genes. A novel mutation in COL1A1, c.700delG, was detected by genomic DNA sequencing in the mother and daughter, but not in their relatives. The identification of this mutation led to the conclusion that they were affected by mild OI type I. Open reading frame analysis indicated that this frameshift mutation would truncate α1-chain type I collagen at residue p263 (p.E234KfsX264), while the wild-type protein would contain 1,464 residues. The clinical data were consistent with the patients’ diagnosis of mild OI type I caused by haploinsufficiency of α1-chain type I collagen. Combined with previous reports, identification of the novel mutation COL1A1-c.700delG in these patients suggests that additional genetic and environmental factors may influence the severity of OI. PMID:25983617

  14. Alternative Fuels for Washington's School Buses: A Report to the Washington State Legislature.

    Science.gov (United States)

    Lyons, John Kim; McCoy, Gilbert A.

    This document presents findings of a study that evaluated the use of both propane and compressed natural gas as alternative fuels for Washington State school buses. It discusses air quality improvement actions by state- and federal-level regulators and summarizes vehicle design, development, and commercialization activities by all major engine,…

  15. University of Washington Center for Child Environmental Health Risks Research

    Data.gov (United States)

    Federal Laboratory Consortium — The theme of the University of Washington based Center for Child Environmental Health Risks Research (CHC) is understanding the biochemical, molecular and exposure...

  16. The impact of ColRS two-component system and TtgABC efflux pump on phenol tolerance of Pseudomonas putida becomes evident only in growing bacteria

    Directory of Open Access Journals (Sweden)

    Kivisaar Maia

    2010-04-01

    Full Text Available Abstract Background We have recently found that Pseudomonas putida deficient in ColRS two-component system is sensitive to phenol and displays a serious defect on solid glucose medium where subpopulation of bacteria lyses. The latter phenotype is significantly enhanced by the presence of phenol in growth medium. Here, we focused on identification of factors affecting phenol tolerance of the colR-deficient P. putida. Results By using transposon mutagenesis approach we identified a set of phenol-tolerant derivatives of colR-deficient strain. Surprisingly, half of independent phenol tolerant clones possessed miniTn5 insertion in the ttgABC operon. However, though inactivation of TtgABC efflux pump significantly enhanced phenol tolerance, it did not affect phenol-enhanced autolysis of the colR mutant on glucose medium indicating that phenol- and glucose-caused stresses experienced by the colR-deficient P. putida are not coupled. Inactivation of TtgABC pump significantly increased the phenol tolerance of the wild-type P. putida as well. Comparison of phenol tolerance of growing versus starving bacteria revealed that both ColRS and TtgABC systems affect phenol tolerance only under growth conditions and not under starvation. Flow cytometry analysis showed that phenol strongly inhibited cell division and to some extent also caused cell membrane permeabilization to propidium iodide. Single cell analysis of populations of the ttgC- and colRttgC-deficient strains revealed that their membrane permeabilization by phenol resembles that of the wild-type and the colR mutant, respectively. However, cell division of P. putida with inactivated TtgABC pump seemed to be less sensitive to phenol than that of the parental strain. At the same time, cell division appeared to be more inhibited in the colR-mutant strain than in the wild-type P. putida. Conclusions ColRS signal system and TtgABC efflux pump are involved in the phenol tolerance of P. putida. However, as

  17. Molecular Evolution and Phylogenetic Analysis of Eight COL Superfamily Genes in Group I Related to Photoperiodic Regulation of Flowering Time in Wild and Domesticated Cotton (Gossypium) Species

    Science.gov (United States)

    Zhang, Rui; Ding, Jian; Liu, Chunxiao; Cai, Caiping; Zhou, Baoliang; Zhang, Tianzhen; Guo, Wangzhen

    2015-01-01

    Flowering time is an important ecological trait that determines the transition from vegetative to reproductive growth. Flowering time in cotton is controlled by short-day photoperiods, with strict photoperiod sensitivity. As the CO-FT (CONSTANS-FLOWER LOCUS T) module regulates photoperiodic flowering in several plants, we selected eight CONSTANS genes (COL) in group I to detect their expression patterns in long-day and short-day conditions. Further, we individually cloned and sequenced their homologs from 25 different cotton accessions and one outgroup. Finally, we studied their structures, phylogenetic relationship, and molecular evolution in both coding region and three characteristic domains. All the eight COLs in group I show diurnal expression. In the orthologous and homeologous loci, each gene structure in different cotton species is highly conserved, while length variation has occurred due to insertions/deletions in intron and/or exon regions. Six genes, COL2 to COL5, COL7 and COL8, exhibit higher nucleotide diversity in the D-subgenome than in the A-subgenome. The Ks values of 98.37% in all allotetraploid cotton species examined were higher in the A-D and At-Dt comparison than in the A-At and D-Dt comparisons, and the Pearson’s correlation coefficient (r) of Ks between A vs. D and At vs. Dt also showed positive, high correlations, with a correlation coefficient of at least 0.797. The nucleotide polymorphism in wild species is significantly higher compared to G. hirsutum and G. barbadense, indicating a genetic bottleneck associated with the domesticated cotton species. Three characteristic domains in eight COLs exhibit different evolutionary rates, with the CCT domain highly conserved, while the B-box and Var domain much more variable in allotetraploid species. Taken together, COL1, COL2 and COL8 endured greater selective pressures during the domestication process. The study improves our understanding of the domestication-related genes/traits during cotton

  18. Variação do volume de gotas de colírios lubrificantes disponíveis no mercado brasileiro

    Directory of Open Access Journals (Sweden)

    Vitorugo Silvestre Nascimento

    Full Text Available RESUMO Objetivo: Avaliar a variação intra e interexaminadores do volume de gotas dispensados de frascos de colírios lubrificantes disponíveis no mercado. Métodos: Foram estudados cinco frascos de colírios lubrificantes e dezenove voluntários participaram deste estudo. A massa média de gotas de 20µl dos colírios foi obtida utilizando micropipeta e balança de precisão e como padrão para comparação com a massa das gotas obtidas pelos voluntários. Cinco gotas de cada frasco foram pesadas individualmente com o tubo de colírio perpendicular à balança, usando o primeiro e segundo dedos da mão direita, de forma que a pressão fosse aplicada somente no meio do frasco. Os experimentos foram realizados em uma sala climatizada a temperatura ambiente (21±1°C. Resultados: Todos os frascos de colírios apresentaram variação estatisticamente significante das massas das gotas obtidas pelos examinadores quando comparadas com a massa média padrão de 0,0182±0,0014g, com exceção da comparação entre os dados do colírio A com o colírio D, que não apresentou variação estatisticamente significante. Conclusão: O presente estudo demonstra a ausência de uniformidade das gotas dispensadas pelos frascos de colírios disponíveis no mercado e a sua inadequação à real necessidade, uma vez que as gotas dispensadas são maiores do que o indicado. Esse fato torna-se um problema quando se trata de período de tratamento prolongado, especialmente com colírios dispendiosos como os indicados para a terapêutica do glaucoma. Nesse sentido, a padronização das gotas de colírios se faz necessária.

  19. The generation and characterization of novel Col1a1FRT-Cre-ER-T2-FRT and Col1a1FRT-STOP-FRT-Cre-ER-T2 mice for sequential mutagenesis

    Science.gov (United States)

    Zhang, Minsi; Kirsch, David G.

    2015-01-01

    ABSTRACT Novel genetically engineered mouse models using the Cre-loxP or the Flp-FRT systems have generated useful reagents to manipulate the mouse genome in a temporally-regulated and tissue-specific manner. By incorporating a constitutive Cre driver line into a mouse model in which FRT-regulated genes in other cell types are regulated by Flp-FRT recombinase, gene expression can be manipulated simultaneously in separate tissue compartments. This application of dual recombinase technology can be used to dissect the role of stromal cells in tumor development and cancer therapy. Generating mice in which Cre-ERT2 is expressed under Flp-FRT-mediated regulation would enable step-wise manipulation of the mouse genome using dual recombinase technology. Such next-generation mouse models would enable sequential mutagenesis to better model cancer and define genes required for tumor maintenance. Here, we generated novel genetically engineered mice that activate or delete Cre-ERT2 in response to Flp recombinase. To potentially utilize the large number of Cre-loxP-regulated transgenic alleles that have already been targeted into the Rosa26 locus, such as different reporters and mutant genes, we targeted the two novel Cre-ERT2 alleles into the endogenous Col1a1 locus for ubiquitous expression. In the Col1a1FRT-Cre-ER-T2-FRT mice, Flp deletes Cre-ERT2, so that Cre-ERT2 is only expressed in cells that have never expressed Flp. In contrast, in the Col1a1FRT-STOP-FRT-Cre-ER-T2 mice, Flp removes the STOP cassette to allow Cre-ERT2 expression so that Cre-ERT2 is only expressed in cells that previously expressed Flp. These two new novel mouse strains will be complementary to each other and will enable the exploration of complex biological questions in development, normal tissue homeostasis and cancer. PMID:26183214

  20. The generation and characterization of novel Col1a1FRT-Cre-ER-T2-FRT and Col1a1FRT-STOP-FRT-Cre-ER-T2 mice for sequential mutagenesis

    Directory of Open Access Journals (Sweden)

    Minsi Zhang

    2015-09-01

    Full Text Available Novel genetically engineered mouse models using the Cre-loxP or the Flp-FRT systems have generated useful reagents to manipulate the mouse genome in a temporally-regulated and tissue-specific manner. By incorporating a constitutive Cre driver line into a mouse model in which FRT-regulated genes in other cell types are regulated by Flp-FRT recombinase, gene expression can be manipulated simultaneously in separate tissue compartments. This application of dual recombinase technology can be used to dissect the role of stromal cells in tumor development and cancer therapy. Generating mice in which Cre-ERT2 is expressed under Flp-FRT-mediated regulation would enable step-wise manipulation of the mouse genome using dual recombinase technology. Such next-generation mouse models would enable sequential mutagenesis to better model cancer and define genes required for tumor maintenance. Here, we generated novel genetically engineered mice that activate or delete Cre-ERT2 in response to Flp recombinase. To potentially utilize the large number of Cre-loxP-regulated transgenic alleles that have already been targeted into the Rosa26 locus, such as different reporters and mutant genes, we targeted the two novel Cre-ERT2 alleles into the endogenous Col1a1 locus for ubiquitous expression. In the Col1a1FRT-Cre-ER-T2-FRT mice, Flp deletes Cre-ERT2, so that Cre-ERT2 is only expressed in cells that have never expressed Flp. In contrast, in the Col1a1FRT-STOP-FRT-Cre-ER-T2 mice, Flp removes the STOP cassette to allow Cre-ERT2 expression so that Cre-ERT2 is only expressed in cells that previously expressed Flp. These two new novel mouse strains will be complementary to each other and will enable the exploration of complex biological questions in development, normal tissue homeostasis and cancer.

  1. Greenhouse gas mitigation options for Washington State

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, N.

    1996-04-01

    President Clinton, in 1993, established a goal for the United States to return emissions of greenhouse gases to 1990 levels by the year 2000. One effort established to help meet this goal was a three part Environmental Protection Agency state grant program. Washington State completed part one of this program with the release of the 1990 greenhouse gas emissions inventory and 2010 projected inventory. This document completes part two by detailing alternative greenhouse gas mitigation options. In part three of the program EPA, working in partnership with the States, may help fund innovative greenhouse gas reduction strategies. The greenhouse gas control options analyzed in this report have a wide range of greenhouse gas reductions, costs, and implementation requirements. In order to select and implement a prudent mix of control strategies, policy makers need to have some notion of the potential change in climate, the consequences of that change and the uncertainties contained therein. By understanding the risks of climate change, policy makers can better balance the use of scarce public resources for concerns that are immediate and present against those that affect future generations. Therefore, prior to analyzing alternative greenhouse gas control measures, this report briefly describes the phenomenon and uncertainties of global climate change, and then projects the likely consequences for Washington state.

  2. Washington State University Algae Biofuels Research

    Energy Technology Data Exchange (ETDEWEB)

    chen, Shulin [Washington State Univ., Pullman, WA (United States). Dept. of Biological Systems Engineering; McCormick, Margaret [Targeted Growth, Inc., Seattle, WA (United States); Sutterlin, Rusty [Inventure Renewables, Inc., Gig Harbor, WA (United States)

    2012-12-29

    The goal of this project was to advance algal technologies for the production of biofuels and biochemicals by establishing the Washington State Algae Alliance, a collaboration partnership among two private companies (Targeted Growth, Inc. (TGI), Inventure Chemicals (Inventure) Inc (now Inventure Renewables Inc) and Washington State University (WSU). This project included three major components. The first one was strain development at TGI by genetically engineering cyanobacteria to yield high levels of lipid and other specialty chemicals. The second component was developing an algal culture system at WSU to produce algal biomass as biofuel feedstock year-round in the northern states of the United States. This system included two cultivation modes, the first one was a phototrophic process and the second a heterotrophic process. The phototrophic process would be used for algae production in open ponds during warm seasons; the heterotrophic process would be used in cold seasons so that year-round production of algal lipid would be possible. In warm seasons the heterotrophic process would also produce algal seeds to be used in the phototrophic culture process. Selected strains of green algae and cyanobacteria developed by TGI were tested in the system. The third component was downstream algal biomass processing by Inventure that included efficiently harvesting the usable fuel fractions from the algae mass and effectively isolating and separating the usable components into specific fractions, and converting isolated fractions into green chemicals.

  3. Evolution of a highly vulnerable ice-cored moraine: Col des Gentianes, Swiss Alps

    Science.gov (United States)

    Ravanel, L.; Lambiel, C.; Oppikofer, T.; Mazotti, B.; Jaboyedoff, M.

    2012-04-01

    Rock mass movements are dominant in the morphodynamics of high mountain rock slopes and are at the origin of significant risks for people who attend these areas and for infrastructures that are built on (mountain huts, cable cars, etc.). These risks are becoming greater because of permafrost degradation and glacier retreat, two consequences of the global warming. These two commonly associated factors may affect slope stability by changing mechanical properties of the interstitial ice and modifying the mechanical constraints in these rock slopes. Between 1977 and 1979, significant works were carried out on the Little Ice Age moraine of the Tortin glacier at the Col des Gentianes (2894 m), in the Mont Fort area (Verbier, Switzerland), for the construction of a cable car station and a restaurant. Since the early 1980s, the glacier drastically retreated and the moraine became unstable: its inner slope has retreated for several meters. Various observations and geoelectric measurements indicate that significant volume of massive ice mass is still present within the moraine (ice-cored moraine). Its melting could therefore increase the instability of the moraine. Since 2007, the moraine is surveyed by terrestrial laser scanning (TLS) in order to characterize its evolution: 8 campaigns were conducted between July 2007 and October 2011. The comparison of the high resolution 3D models so obtained allowed the detection and quantification of mass movements that have affected the moraine over this period, essentially by calculating difference maps (shortest oblique distances between two models). Between July 2007 and October 2011, 7 landslides were measured, involving volumes between 87 and 1138 m3. The most important of these occurred during the summers 2009 and 2011. TLS data also allowed identifying: (i) two main areas affected by slower but sometimes substantial movements (displacements of blocks on more than 2 m during a summer period); (ii) significant deposits of

  4. A mobilidade humana na tríplice fronteira: Peru, Brasil e Colômbia

    Directory of Open Access Journals (Sweden)

    Márcia Maria de Oliveira

    2006-08-01

    Full Text Available OS MOVIMENTOS migratórios nas fronteiras amazônicas ainda são pouco abordados, tanto pela academia quanto pelas instituições que lidam com a temática da mi-gração. Há elementos novos que configuram características peculiares à mobilidade humana na tríplice fronteira Peru-Colômbia-Brasil que merecem uma abordagem mais profunda do ponto de vista dos estudos migratórios. Atualmente, há fluxos conside-ráveis de migração internacional nessa região adentrando na fronteira brasileira, desafiando o Estado brasileiro a implementar uma política migratória que consiga lidar com fenômenos, tais como a presença de peruanos em situação irregular em território brasileiro, a mobilidade dos povos indígenas nas regiões de fronteira e ainda, mais recentemente, a entrada crescente de colombianos desplazados pela guerrilha interna que pedem refúgio ao Estado brasileiro.THE MIGRATORY mobility in the Amazonian borders is less than enough investigated, neither by the academy nor by the institutions that deal with the migration question. There are new elements that depict peculiar characteristics to the human mobility in the triple border of Peru, Colombia and Brazil, that deserve a deeper investigation from the view point of the migratory studies. At the moment, it has conside-rable flows of international migration in this region moving in the Brazilian border, challenging the Brazilian State to implement a migratory policy that is able to deal with the phenomena such as the presence of Peruvians in an irregular situation in Brazilian territory, the mobility of the indigenous peoples in the border regions and still, more recently, the increasing entrance of the Colombians desplazados through the internal guerrilla who ask for shelter to the Brazilian State.

  5. 75 FR 14462 - Notice of Inventory Completion: Central Washington University, Department of Anthropology...

    Science.gov (United States)

    2010-03-25

    ... Washington University, Department of Anthropology, Ellensburg, WA, and Thomas Burke Memorial Washington State... Washington University, Department of Anthropology, Ellensburg, WA, and the Thomas Burke Memorial Washington... Anthropology, Central Washington University, Ellensburg, WA 98926-7544, telephone (509) 963-2671 or Dr. Peter...

  6. ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice

    Directory of Open Access Journals (Sweden)

    Frances E. Jones

    2016-02-01

    Full Text Available Collagen IV is a major component of basement membranes, and mutations in COL4A1, which encodes collagen IV alpha chain 1, cause a multisystemic disease encompassing cerebrovascular, eye and kidney defects. However, COL4A1 renal disease remains poorly characterized and its pathomolecular mechanisms are unknown. We show that Col4a1 mutations in mice cause hypotension and renal disease, including proteinuria and defects in Bowman's capsule and the glomerular basement membrane, indicating a role for Col4a1 in glomerular filtration. Impaired sodium reabsorption in the loop of Henle and distal nephron despite elevated aldosterone levels indicates that tubular defects contribute to the hypotension, highlighting a novel role for the basement membrane in vascular homeostasis by modulation of the tubular response to aldosterone. Col4a1 mutations also cause diabetes insipidus, whereby the tubular defects lead to polyuria associated with medullary atrophy and a subsequent reduction in the ability to upregulate aquaporin 2 and concentrate urine. Moreover, haematuria, haemorrhage and vascular basement membrane defects confirm an important vascular component. Interestingly, although structural and compositional basement membrane defects occurred in the glomerulus and Bowman's capsule, no tubular basement membrane defects were detected. By contrast, medullary atrophy was associated with chronic ER stress, providing evidence for cell-type-dependent molecular mechanisms of Col4a1 mutations. These data show that both basement membrane defects and ER stress contribute to Col4a1 renal disease, which has important implications for the development of treatment strategies for collagenopathies.

  7. Association of COL1A1 rs1800012 polymorphism with musculoskeletal degenerative diseases: a meta-analysis.

    Science.gov (United States)

    Zhong, Binlong; Huang, Donghua; Ma, Kaige; Deng, Xiangyu; Shi, Deyao; Wu, Fashuai; Shao, Zengwu

    2017-09-26

    It has been reported that the single nucleotide polymorphism (SNP) rs1800012 in COL1A1 gene might be linked to the susceptibility of musculoskeletal degenerative diseases, such as osteoarthritis (OA) and intervertebral disc degeneration (IVDD). However, the data from different studies is contradictory. Here we aimed to comprehensively summarize and clarify the relationship between the SNP and musculoskeletal degenerative diseases. Seven eligible studies including 1339 cases and 5406 controls were screened out from PubMed, Web Of Science and Cochrane library databases. Significant association was identified in sub group analysis of IVDD in homozygote model (GG versus TT: OR = 0.33, 95% CI 0.14-0.78, P = 0.012), heterozygote model (GT versus TT: OR = 0.29, 95% CI 0.11-0.72, P = 0.008) and dominant model (GG/GT versus TT: OR = 0.31, 95% CI 0.13-0.74, P = 0.008). Additionally, significant relationship was also found in sub group analysis of severe degree of IVDD in homozygote model (GG versus TT: OR = 0.37, 95% CI 0.15-0.91, P = 0.031), heterozygote model (GT versus TT: OR = 0.33, 95% CI 0.13-0.87, P = 0.024) and dominant model (GG/GT versus TT: OR = 0.36, 95% CI 0.14-0.88, P = 0.025). Although no significance was observed, there is a trend that the more G allele at COL1A1 rs1800012 site, the less possibility of IVDD and severe IVDD would happen. Our results indicate that COL1A1 rs1800012 polymorphism associates with the susceptibility of IVDD. However, this polymorphism may not be associated with OA risk.

  8. Whole exome analysis identifies dominant COL4A1 mutations in patients with complex ocular phenotypes involving microphthalmia.

    Science.gov (United States)

    Deml, B; Reis, L M; Maheshwari, M; Griffis, C; Bick, D; Semina, E V

    2014-11-01

    Anophthalmia/microphthalmia (A/M) is a developmental ocular malformation defined as complete absence or reduction in size of the eye. A/M is a heterogenous disorder with numerous causative genes identified; however, about half the cases lack a molecular diagnosis. We undertook whole exome sequencing in an A/M family with two affected siblings, two unaffected siblings, and unaffected parents; the ocular phenotype was isolated with only mild developmental delay/learning difficulties reported and a normal brain magnetic resonance imaging (MRI) in the proband at 16 months. No pathogenic mutations were identified in 71 known A/M genes. Further analysis identified a shared heterozygous mutation in COL4A1, c.2317G>A, p.(Gly773Arg) that was not seen in the unaffected parents and siblings. Analysis of 24 unrelated A/M exomes identified a novel c.2122G>A, p.(Gly708Arg) mutation in an additional patient with unilateral microphthalmia, bilateral microcornea and Peters anomaly; the mutation was absent in the unaffected mother and the unaffected father was not available. Mutations in COL4A1 have been linked to a spectrum of human disorders; the most consistent feature is cerebrovascular disease with variable ocular anomalies, kidney and muscle defects. This study expands the spectrum of COL4A1 phenotypes and indicates screening in patients with A/M regardless of MRI findings or presumed inheritance pattern. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  9. Detection of mutations in the COL4A5 gene by SSCP in X-linked Alport syndrome

    DEFF Research Database (Denmark)

    Hertz, Jens Michael; Juncker, I; Persson, U

    2001-01-01

    , three in-frame deletions, four nonsense mutations, and six splice site mutations. Twenty-two of the mutations have not previously been reported. Furthermore, we found one non-pathogenic amino acid substitution, one rare variant in a non-coding region, and one polymorphism with a heterozygosity of 28...... of type IV-collagen. We performed mutation analysis of the COL4A5 gene by PCR-SSCP analysis of each of the 51 exons with flanking intronic sequences in 81 patients suspected of X-linked Alport syndrome including 29 clear X-linked cases, 37 cases from families with a pedigree compatible with X...

  10. Xarxes educatives 2.1. Mitjans socials, entorns col·laboratius i processos d'ensenyament i aprenentatge

    OpenAIRE

    Levis, Diego; Diéguez, Sol; Rey, Eugenia

    2011-01-01

    El projecte Xarxes Educatives 2.1 es proposa indagar les possibilitats que ofereixen els mitjans socials i els entorns col·laboratius a internet en els processos d'ensenyament i aprenentatge. Per a això, en cursos presencials i en cursos de capacitació docent de modalitat semipresencial fem servir una aplicació de la web 2.0 destinada a la creació de xarxes socials. L'aplicació es va configurar d'acord amb els objectius pedagògics de cada un dels cursos inclosos en el projecte. Es va establir...

  11. 77 FR 37317 - Drawbridge Operation Regulation; Lake Washington, Seattle, WA

    Science.gov (United States)

    2012-06-21

    ..., 2012. The deviation allows the floating draw span of the SR 520 Lake Washington Bridge to remain in the... schedule that governs the State Route 520 (SR 520) Bridge across Lake Washington at Seattle, WA. This... allows the bridge to remain in the closed position to allow safe movement of event participants. DATES...

  12. To Be a Slave: The Boyhood of Booker T. Washington.

    Science.gov (United States)

    National Park Service (Dept. of Interior), Washington, DC.

    The Booker T. Washington National Monument preserves and protects the birth site and childhood home of Booker T. Washington, while interpreting his life experiences and significance in U.S. history as the most powerful African American between 1895 and 1915. The park provides a resource for public education and a focal point for continuing…

  13. Educator Supply and Demand in Washington State. 2004 Report

    Science.gov (United States)

    Lashway, Larry; Maloney, Rick; Hathaway, Randy; Bryant, B. J.

    2005-01-01

    This report describes the findings of the third Educator Supply and Demand Research study in the State of Washington. The intent of these Washington studies is to provide data to inform and shape decisions and activities in the following ways: (1) Provide useful information for educational policymakers, including the legislature, the State Board…

  14. Expanding Access and Opportunity: The Washington State Achievers Program

    Science.gov (United States)

    Ramsey, Jennifer; Gorgol, Laura

    2010-01-01

    In 2001, the Bill & Melinda Gates Foundation launched a 10-year, multi-million dollar initiative, the Washington State Achievers Program (WSA), to increase opportunities for low-income students to attend postsecondary institutions in Washington State. The Bill & Melinda Gates Foundation granted funds to the College Success Foundation…

  15. Expanding Access and Opportunity: The Washington State Achievers Scholarship

    Science.gov (United States)

    O'Brien, Colleen

    2011-01-01

    In 2001, the Bill & Melinda Gates Foundation launched the multi-year, multi-million dollar Washington State Achievers Scholarship program. Concerned about disparities in college participation for low-income students in the state of Washington versus their wealthier peers, the Gates Foundation partnered with the College Success Foundation…

  16. 75 FR 71139 - Land Acquisitions; Puyallup Tribe of Washington

    Science.gov (United States)

    2010-11-22

    ... Auditor, in Pierce County, Washington. Except that portion of Lot 3 conveyed to the State of Washington by Deeds recorded under Auditor's file number 689865 and 689858. Together with the East half of the... amendment of Short Plat Nos. 8502210395 and 8403080186, filed with the Pierce County Auditor, in Pierce...

  17. Integrated solid waste management of Seattle, Washington

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-11-01

    The subject document reports the results of an in-depth investigation of the fiscal year 1992 cost of the City of Seattle, Washington, integrated municipal solid waste management (IMSWM) system, the energy consumed to operate the system, and the environmental performance requirements for each of the system`s waste-processing and disposal facilities. Actual data from records kept by participants is reported in this document. Every effort was made to minimize the use of assumptions, and no attempt is made to interpret the data reported. Analytical approaches are documented so that interested analysts may perform manipulation or further analysis of the data. As such, the report is a reference document for MSW management professionals who are interested in the actual costs and energy consumption for a one-year period, of an operating IMSWM systems.

  18. Nuclear terrorism: after the Washington summit

    International Nuclear Information System (INIS)

    Hautecouverture, Benjamin

    2016-01-01

    In this note, the author comments the issues addressed by the 2016 Nuclear Safety Summit (NSS) of Washington, and the content of its final statement. He notices that the scope of addressed topics has evolved since the first summits (issues related to highly enriched uranium and to plutonium), that not only technological but also political and diplomatic issues are taken into account, and that GNOs are always more involved. The author briefly comments some aspects of the content of the final statement: threat of nuclear terrorism, improvement of nuclear safety since 2010, recall of the three main pillars of the non proliferation Treaty (non proliferation, disarmament, specific uses of nuclear energy), implementation of nuclear safety under at the own responsibility and duty of countries possessing nuclear materials. Finally, the author discusses how the NSS process will go on, and evokes remaining questions regarding the existence of an actual international constraining regime, and financial and functional issues

  19. Water resources of King County, Washington

    Science.gov (United States)

    Richardson, Donald; Bingham, J.W.; Madison, R.J.; Williams, R.

    1968-01-01

    Although the total supply of water in King County is large, water problems are inevitable because of the large and rapidly expanding population. The county contains a third of the 3 million people in Washington, most of the population being concentrated in the Seattle metropolitan area. King County includes parts of two major physiographic features: the western area is part of the Puget Sound Lowland, and the eastern area is part of the Cascade Range. In these two areas, the terrain, weather, and natural resources (including water) contrast markedly. Average annual precipitation in the county is about 80 inches, ranging from about 30 inches near Puget Sound to more than 150 inches in parts of the Cascades. Annual evapotranspiration is estimated to range from 15 to 24 inches. Average annual runoff ranges from about 15 inches in the lowlands to more than 100 inches in the mountains. Most of the streamflow is in the major basins of the county--the Green-Duwamish, Lake Washington, and Snoqualmie basins. The largest of these is the Snoqualmie River basin (693 square miles), where average annual runoff during the period 1931-60 was about 79 inches. During the same period, annual runoff in the Lake Washington basin ( 607 square miles) averaged about 32 inches, and in the Green-Duwamish River basin (483 square miles), about 46 inches. Seasonal runoff is generally characterized by several high-flow periods in the winter, medium flows in the spring, and sustained low flows in the summer and fall. When floods occur in the county they come almost exclusively between October and March. The threat of flood damage is greatest on the flood plaits of the larger rivers, but in the Green-Duwamish Valley the threat was greatly reduced with the completion of Howard A. Hanson Dam in 1962. In the Snoqualmie River basin, where no such dam exists, the potential damage from a major flood increases each year as additional land is developed in the Snoqualmie Valley. 0nly moderate amounts of

  20. Avaliação do custo de colírios lubrificantes a base exclusivamente de carboximetilcelulose no mercado brasileiro

    Directory of Open Access Journals (Sweden)

    Paulo Estacia

    2013-10-01

    Full Text Available OBJETIVO: Avaliar o custo do uso dos colírios lubrificantes mais utilizados no mercado nacional que apresentam na formulação o mesmo princípio ativo - carboximetilcelulose, considerando o número total de gotas presente em cada frasco dos colírios. MÉTODOS: Foi realizado um estudo experimental utilizando três frascos de cada um dos colírios Lacrifilm® (colírio 1 e Fresh Tears® (colírio 2. Para análise do custo dos colírios contou-se o número de gotas de cada frasco correlacionando os preços dos mesmos. O preço considerado para cada medicação foi o mínimo ao consumidor com alíquota de 17% publicado para o mês de janeiro de 2012. A análise estatística foi efetuada em SPSS® 18. A comparação das variáveis quantitativas analisadas foi procedida através do teste não paramétrico Mann-Whitney e correlação linear de Spearman, sendo considerada uma diferença estatisticamente significante um valor de probabilidade inferior a 0,05. RESULTADOS: Verificou-se que há diferença estatisticamente significativa entre as marcas de colírios avaliados com relação ao número total de gotas. O colírio 1 apresentou o maior número de gotas. CONCLUSÃO: O colírio 1, que tem o maior número de gotas por frasco, também é o que tem o preço mais acessível. Portanto, verifica-se que é o produto mais econômico, ou seja, o paciente pagará menos por cada gota.

  1. PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene Capture Platforms

    Directory of Open Access Journals (Sweden)

    Manuel Ares-Arroyo

    2018-03-01

    Full Text Available ColE1 plasmids are important vehicles for the spread of antibiotic resistance in the Enterobacteriaceae and Pasteurellaceae families of bacteria. Their monitoring is essential, as they harbor important resistant determinants in humans, animals and the environment. In this work, we have analyzed ColE1 replicons using bioinformatic and experimental approaches. First, we carried out a computational study examining the structure of different ColE1 plasmids deposited in databases. Bioinformatic analysis of these ColE1 replicons revealed a mosaic genetic structure consisting of a host-adapted conserved region responsible for the housekeeping functions of the plasmid, and a variable region encoding a wide variety of genes, including multiple antibiotic resistance determinants. From this exhaustive computational analysis we developed a new PCR-based technique, targeting a specific sequence in the conserved region, for the screening, capture and sequencing of these small plasmids, either specific for Enterobacteriaceae or specific for Pasteurellaceae. To validate this PCR-based system, we tested various collections of isolates from both bacterial families, finding that ColE1 replicons were not only highly prevalent in antibiotic-resistant isolates, but also present in susceptible bacteria. In Pasteurellaceae, ColE1 plasmids carried almost exclusively antibiotic resistance genes. In Enterobacteriaceae, these plasmids encoded a large range of traits, including not only antibiotic resistance determinants, but also a wide variety of genes, showing the huge genetic plasticity of these small replicons. Finally, we also used a metagenomic approach in order to validate this technique, performing this PCR system using total DNA extractions from fecal samples from poultry, turkeys, pigs and humans. Using Illumina sequencing of the PCR products we identified a great diversity of genes encoded by ColE1 replicons, including different antibiotic resistance

  2. 77 FR 11582 - Notice of Inventory Completion: Central Washington University Department of Anthropology...

    Science.gov (United States)

    2012-02-27

    ...: Central Washington University Department of Anthropology, Ellensburg, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Central Washington University Department of Anthropology has... Central Washington University Department of Anthropology. Repatriation of the human remains and associated...

  3. 77 FR 15802 - Notice of Inventory Completion: Central Washington University Department of Anthropology...

    Science.gov (United States)

    2012-03-16

    ...: Central Washington University Department of Anthropology, Ellensburg, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Central Washington University Department of Anthropology has... contact the Central Washington University Department of Anthropology. Repatriation of the human remains to...

  4. 75 FR 14463 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

    Science.gov (United States)

    2010-03-25

    ... Washington, Department of Anthropology, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice... University of Washington, Department of Anthropology, Seattle, WA. The human remains were removed from... University of Washington, Department of Anthropology and Burke Museum staff in consultation with...

  5. Space Radar Image of Wenatchee, Washington

    Science.gov (United States)

    1994-01-01

    This spaceborne radar image shows a segment of the Columbia River as it passes through the area of Wenatchee, Washington, about 220 kilometers (136 miles) east of Seattle. The Wenatchee Mountains, part of the Cascade Range, are shown in green at the lower left of the image. The Cascades create a 'rain shadow' for the region, limiting rainfall east of the range to less than 26 centimeters (10 inches) per year. The radar's ability to see different types of vegetation is highlighted in the contrast between the pine forests, that appear in green and the dry valley plain that shows up as dark purple. The cities of Wenatchee and East Wenatchee are the grid-like areas straddling the Columbia River in the left center of the image. With a population of about 60,000, the region produces about half of Washington state's lucrative apple crop. Several orchard areas appear as green rectangular patches to the right of the river in the lower right center. Radar images such as these can be used to monitor land use patterns in areas such as Wenatchee, that have diverse and rapidly changing urban, agricultural and wild land pressures. This image was acquired by Spaceborne Imaging Radar-C/X-Band Synthetic Aperture Radar (SIR-C/X-SAR) onboard the space shuttle Endeavour on October 10, 1994. The image is 38 kilometers by 45 kilometers (24 miles by 30 miles) and is centered at 47.3 degrees North latitude, 120.1 degrees West longitude. North is toward the upper left. The colors are assigned to different radar frequencies and polarizations of the radar as follows: red is L-band, horizontally transmitted and received; green is L-band, horizontally transmitted, vertically received; and blue is C-band, horizontally transmitted, vertically received. SIR-C/X-SAR, a joint mission of the German, Italian, and United States space agencies, is part of NASA's Mission to Planet Earth.

  6. Metro de Washington EE.UU.

    Directory of Open Access Journals (Sweden)

    Weese, Harry

    1979-09-01

    Full Text Available This article describes the works involved in the first stage of the Washington Underground (Subway system which was begun in 1969 and scheduled for completion in 1983 and is the most modern metropolitan railway in North America. Lines have double track and will carry three million passengers daily. Different construction methods have been used throughout: tunnel formed try digging a trench then roofed and covered, excavated tunnel and elevated structures. Stations features answer to the strictest demands, provided with closed circuit television, air conditioning, noise dampening Systems, special access ways, fire protection Systems and automatic traffic control. Special attention is given to the two bridges over the Pentagon and over the Anacostia, pointing out their differences and the elevated structure at the National Airport.

    Se describen en este articulo los trabajos de la primera fase del Metro de Washington que, iniciado en el año 1969 será, a su terminación en el año 1983, el más moderno sistema de ferrocarril metropolitano de Norte América. Es de doble carril y servirá para tres millones de usuarios. Se han empleado distintos sistemas de obra en su realización: túnel artificial realizado mediante una zanja que después se cubre; túnel perforado, y estructuras aéreas. Las características de las estaciones responden a las mayores exigencias, pues tienen circuito cerrado de televisión, aire acondicionado, sistemas para atenuar el ruido, accesos especiales, sistema de protección contra el fuego y control automático del Metro. Se estudian de un modo particular: los dos puentes sobre el Pentágono y el Anacostia, señalando sus diferencias y la estructura aérea del Aeropuerto Nacional.

  7. A PTEN-COL17A1 fusion gene and its novel regulatory role in Collagen XVII expression and GBM malignance.

    Science.gov (United States)

    Yan, Xiaoyan; Zhang, Chuanbao; Liang, Tingyu; Yang, Fan; Wang, Haoyuan; Wu, Fan; Wang, Wen; Wang, Zheng; Cheng, Wen; Xu, Jiangnan; Jiang, Tao; Chen, Jing; Ding, Yaozhong

    2017-10-17

    Collagen XVII expression has recently been demonstrated to be correlated with the tumor malignance. While Collagen XVII is known to be widely distributed in neurons of the human brain, its precise role in pathogenesis of glioblastoma multiforme (GBM) is unknown. In this study, we identified and characterized a new PTEN-COL17A1 fusion gene in GMB using transcriptome sequencing. Although fusion gene did not result in measurable fusion protein production, its presence is accompanied with high levels of COL17A1 expression, revealed a novel regulatory mechanism of Collagen XVII expression by PTEN-COL17A1 gene fusion. Knocked down Collagen XVII expression in glioma cell lines resulted in decreased tumor invasiveness, along with significant reduction of MMP9 expression, while increased Collagen XVII expression promotes invasive activities of glioma cells and associated with GBM recurrences. Together, our results uncovered a new PTEN-COL17A1 fusion gene and its novel regulatory role in Collagen XVII expression and GBM malignance, and demonstrated that COL17A1 could serve as a useful prognostic biomarker and therapeutic targets for GBM.

  8. A novel nonsteroidal antifibrotic oligo decoy containing the TGF-beta element found in the COL1A1 gene which regulates murine schistosomiasis liver fibrosis.

    Science.gov (United States)

    Boros, D L; Singh, K P; Gerard, H C; Hudson, A P; White, S L; Cutroneo, K R

    2005-08-01

    Schistosomiasis mansoni disseminated worm eggs in mice and humans induce granulomatous inflammations and cumulative fibrosis causing morbidity and possibly mortality. In this study, intrahepatic and I.V. injections of a double-stranded oligodeoxynucleotide decoy containing the TGF-beta regulatory element found in the distal promoter of the COL1A1 gene into worm-infected mice suppressed TGF-beta1, COL1A1, tissue inhibitor of metalloproteinase-1, and decreased COL3A1 mRNAs to a lesser extent. Sequence comparisons within the mouse genome found homologous sequences within the COL3A1, TGF-beta1, and TIMP-1 5' flanking regions. Cold competition gel mobility shift assays using these homologous sequences with 5' and 3' flanking regions found in the natural COL1A1 gene showed competition. Competitive gel mobility assays in a separate experiment showed no competition using a 5-base mutated or scrambled sequence. Explanted liver granulomas from saline-injected mice incorporated 10.45 +/- 1.7% (3)H-proline into newly synthesized collagen, whereas decoy-treated mice showed no collagen synthesis. Compared with the saline control schistosomiasis mice phosphorothioate double-stranded oligodeoxynucleotide treatment decreased total liver collagen content (i.e. hydroxy-4-proline) by 34%. This novel molecular approach has the potential to be employed as a novel antifibrotic treatment modality. (c) 2005 Wiley-Liss, Inc.

  9. Variables físicas, químicas y biológicas del suelo sobre las poblaciones de colémbolos en Costa Rica

    Directory of Open Access Journals (Sweden)

    Cesar Guillen

    2006-01-01

    Full Text Available Se ha discutido que las variables físicas, químicas y biológicas del suelo influyen sobre las poblaciones de colémbolos; sin embargo, la información acerca de la interacción de estas variables, en conjunto, con relación a la diversidad y abundancia de colémbolos, es escasa. En el presente estudio se determinó la relación entre estas variables y la diversidad y abundancia de colémbolos en los suelos de un bosque primario, un bosque secundario y un cafetal en el Parque Nacional Tapantí. El bosque primario presentó los contenidos más altos de materia orgánica y biomasa microbiana, contrario a lo sucedido con la resistencia a la penetración y el pH. Los resultados mostraron una clara asociación entre estas variables y algunas especies de colémbolos, lo cual indica que los cambios en la estructura de las comunidades de colémbolos, por efecto de los cambios en las variables físicas, químicas y biológicas del suelo, pueden ser utilizados como un bioindicador de calidad del suelo y de manejo de los ecosistemas.

  10. Colémbolos asociados con cultivos de pastos en tres zonas de vida

    Directory of Open Access Journals (Sweden)

    Ospina S. Claudia Marcela

    2003-12-01

    Full Text Available

    Los colémbolos son artrópodos muy pequeños,

    sin alas, similares a los insectos y extremadamente

    abundantes en gran cantidad de hábitat, siendo

    uno de los grupos de hexápodos mejor representados

    en el mundo. Son muy importantes por su influencia

    sobre la estructura de algunos suelos, no pocos viven en

    el dosel de las selvas tropicales y algunos actúan como

    dispersores de esporas dentro de los troncos en descomposición.

    En Colombia existen muy pocos estudios de

    reconocimiento de colémbolos. En la literatura se registran

    12 especies en dos localidades cerca de Buenaventura,

    agrupadas en 5 familias; así mismo, han sido

    reconocidas en diferentes estudios otras 7 especies que

    actúan como plagas en Musáceas y en flores cultivadas.

    Con el fin de contribuir al conocimiento de las familias

    de Collembola asociadas con suelos de pastos cultivados

    en el departamento de Antioquia se hicieron muestreos

    en tres zonas de vida de Holdridge (bh-P, bh-MB

    y bs-T en pastos Guinea (

     

    Panicum maximum, Ángleton

  11. Synthesis and Characterization of Hydroxyapatite-Collagen-Chitosan (HA/Col/Chi) Composite Coated on Ti6Al4V

    Science.gov (United States)

    Charlena; Bikharudin, Ahmad; Wahyudi, Setyanto Tri

    2018-01-01

    HA-collagen-chitosan (HA/col/chi) composite is developed to increase bioactivity adhesiveness between the metal and the material composite and to improve corrosion resistance. The Ti6Al4V alloy was coated by soaking in HA/col/chi composite at room temperature and then allowed to stand for 5, 6, and 7 days. Diffraction pattern analysis of the coated Ti6Al4V alloy showed that the dominant phase were HA and Ti6Al4V alloy. Corrosion resistance test in media by using 0.9% NaCl showed the corrosion rate at the level of 0.3567 mpy, which was better than that of the uncoated Ti6Al4V alloy (0.4152 mpy). In vitro cytocompatibility assay on endothelial cell of calf pulmonary artery endothelium (CPAE) (ATCC-CCL 209) showed there was no toxicity in the cell culture with the percent inhibition of 33.33% after 72 hours of incubation.

  12. The expanding spectrum of COL2A1 gene variants IN 136 patients with a skeletal dysplasia phenotype.

    Science.gov (United States)

    Barat-Houari, Mouna; Dumont, Bruno; Fabre, Aurélie; Them, Frédéric Tm; Alembik, Yves; Alessandri, Jean-Luc; Amiel, Jeanne; Audebert, Séverine; Baumann-Morel, Clarisse; Blanchet, Patricia; Bieth, Eric; Brechard, Marie; Busa, Tiffany; Calvas, Patrick; Capri, Yline; Cartault, François; Chassaing, Nicolas; Ciorca, Vidrica; Coubes, Christine; David, Albert; Delezoide, Anne-Lise; Dupin-Deguine, Delphine; El Chehadeh, Salima; Faivre, Laurence; Giuliano, Fabienne; Goldenberg, Alice; Isidor, Bertrand; Jacquemont, Marie-Line; Julia, Sophie; Kaplan, Josseline; Lacombe, Didier; Lebrun, Marine; Marlin, Sandrine; Martin-Coignard, Dominique; Martinovic, Jelena; Masurel, Alice; Melki, Judith; Mozelle-Nivoix, Monique; Nguyen, Karine; Odent, Sylvie; Philip, Nicole; Pinson, Lucile; Plessis, Ghislaine; Quélin, Chloé; Shaeffer, Elise; Sigaudy, Sabine; Thauvin, Christel; Till, Marianne; Touraine, Renaud; Vigneron, Jacqueline; Baujat, Geneviève; Cormier-Daire, Valérie; Le Merrer, Martine; Geneviève, David; Touitou, Isabelle

    2016-07-01

    Heterozygous COL2A1 variants cause a wide spectrum of skeletal dysplasia termed type II collagenopathies. We assessed the impact of this gene in our French series. A decision tree was applied to select 136 probands (71 Stickler cases, 21 Spondyloepiphyseal dysplasia congenita cases, 11 Kniest dysplasia cases, and 34 other dysplasia cases) before molecular diagnosis by Sanger sequencing. We identified 66 different variants among the 71 positive patients. Among those patients, 18 belonged to multiplex families and 53 were sporadic. Most variants (38/44, 86%) were located in the triple helical domain of the collagen chain and glycine substitutions were mainly observed in severe phenotypes, whereas arginine to cysteine changes were more often encountered in moderate phenotypes. This series of skeletal dysplasia is one of the largest reported so far, adding 44 novel variants (15%) to published data. We have confirmed that about half of our Stickler patients (46%) carried a COL2A1 variant, and that the molecular spectrum was different across the phenotypes. To further address the question of genotype-phenotype correlation, we plan to screen our patients for other candidate genes using a targeted next-generation sequencing approach.

  13. Inquérito sobre fungos anemófilos na fronteira Brasil - Colômbia

    Directory of Open Access Journals (Sweden)

    Simão Arão Pecher

    1988-06-01

    Full Text Available Foi realizado um inquérito, sobre fungos anemófllos, em três vilarejos situados na fronteira Brasil-Colômbia, zona muito quente e úmida, durante o mês de julho de 1978. Em apenas uma coleta feita, em diferentes pontos, foram cultivadas colônias pertencentes a dezoito gêneros diferentes de fungos anemófllos, com predominância de Mucor (64,3%, Cândida (54,8%, Rhodotorula (38,1% e Penicillium (38,1%.An inquiry of anemophilous fungi was carried out in small towns located at the Brasilian- Colombian border, a hot and humid zone, during the month of July, 1978. In just one collection, carried out at different sites, colonies were cultivated, and belonged to eighteen different species of anemophilous fungi with predominance of Mucor (64.3%, Candida (54.8%, Rhodotorula (38.1% and Penicillium (38.1%.

  14. Dual reporter transgene driven by 2.3Col1a1 promoter is active in differentiated osteoblasts

    Science.gov (United States)

    Marijanovic, Inga; Jiang, Xi; Kronenberg, Mark S.; Stover, Mary Louise; Erceg, Ivana; Lichtler, Alexander C.; Rowe, David W.

    2003-01-01

    AIM: As quantitative and spatial analyses of promoter reporter constructs are not easily performed in intact bone, we designed a reporter gene specific to bone, which could be analyzed both visually and quantitatively by using chloramphenicol acetyltransferase (CAT) and a cyan version of green fluorescent protein (GFPcyan), driven by a 2.3-kb fragment of the rat collagen promoter (Col2.3). METHODS: The construct Col2.3CATiresGFPcyan was used for generating transgenic mice. Quantitative measurement of promoter activity was performed by CAT analysis of different tissues derived from transgenic animals; localization was performed by visualized GFP in frozen bone sections. To assess transgene expression during in vitro differentiation, marrow stromal cell and neonatal calvarial osteoblast cultures were analyzed for CAT and GFP activity. RESULTS: In mice, CAT activity was detected in the calvaria, long bone, teeth, and tendon, whereas histology showed that GFP expression was limited to osteoblasts and osteocytes. In cell culture, increased activity of CAT correlated with increased differentiation, and GFP activity was restricted to mineralized nodules. CONCLUSION: The concept of a dual reporter allows a simultaneous visual and quantitative analysis of transgene activity in bone.

  15. Toxicidade córneo-conjuntival do colírio de iodo-povidona: estudo experimental

    Directory of Open Access Journals (Sweden)

    Santos Namir Clementino

    2003-01-01

    Full Text Available OBJETIVO: Avaliar a toxicidade ocular do colírio de iodo-povidona a 2,5% e a 0,5% sobre a superfície ocular, na regeneração do epitélio corneal e as alterações histopatológicas da córnea. MÉTODOS: Realizaram-se estudos experimentais consecutivos em coelhos albinos, nos quais se fez a ablação do epitélio de uma área circular central da córnea de 6,5 mm de diâmetro. Em cada experimento foram utilizados 20 animais (40 olhos, sendo que no olho direito foi instilado o colírio de iodo-povidona (caso e no olho esquerdo água destilada (controle, em intervalos de uma hora, durante três dias consecutivos. Durante o experimento, os animais foram submetidos a exames biomicroscópicos diários para avaliação da superfície córneo-conjuntival e realização de fotografias seriadas da área sem epitélio, corada com fluoresceína, para medida da área projetada da lesão com auxílio de analisador de imagem computadorizado. No final do experimento, os animais foram sacrificados para avaliação histopatológica das córneas. RESULTADOS: O colírio de iodo-povidona a 2,5% comprometeu a regeneração epitelial, causou conjuntivite em 100% dos olhos, com produção de secreção de aspecto mucoso em 80%, ceratite ponteada em 40% e edema estrômico leve em 10% dos casos. Os achados histopatológicos foram úlcera de córnea, degeneração hidrópica das células endoteliais e infiltrado inflamatório com predomínio de eosinófilos em 100% dos casos. Nos olhos em que se instilou iodo-povidona a 0,5%, assim como nos controles, observou-se completa regeneração da lesão epitelial (p<0,001 após 72 horas do início do experimento. Do ponto de vista histopatológico, epitelização normal em todos os casos e controles, em apenas um caso observou-se discreto infiltrado de leucócitos perilímbicos. CONCLUSÃO: A toxicidade ocular do colírio de iodo-povidona é dependente da concentração da solução, sendo que o colírio a 2,5% mostrou

  16. 78 FR 2429 - Notice of Inventory Completion: The Museum of Anthropology at Washington State University...

    Science.gov (United States)

    2013-01-11

    ... construction. Archaeologists working for the Washington Archaeological Research Center at Washington State... Reservation. Historical, ethnographic, linguistic, and archaeological information links the site to the...

  17. Osteogenesis imperfecta Type IV: a newly identified variant at position c.560 (G > T; p.Gly187Val) in the COL1A2 gene.

    Science.gov (United States)

    Usta, Akin; Karademir, Dilay; Sen, Eylem; Yazici, Selcuk; Adali, Ertan; Erdem, Erkan; Karacan, Meric

    2017-01-01

    Osteogenesis imperfecta is a clinically heterogenous disease caused by defective collagen syntesis associated with a mutation in the COL1A1 or COL1A2 genes. In this report, we present a case of osteogenesis imperfecta (OI) type IV, seen in a female fetus with incurved femurs at 18 weeks of gestation. Molecular analysis of the newborn revealed a novel mutation at position c.560 (c.560 G > T) of the exon 12 in the COL1A2 gene; which lead to the glycine modification with valine (p.Gly187Val) at codon 187. The pregnancy follow-up was uneventful. After delivery, the newborn underwent biphosponat therapy and no fracture was detected until 1 year old.

  18. Prolonged exposure of methicillin-resistant Staphylococcus aureus (MRSA) COL strain to increasing concentrations of oxacillin results in a multidrug-resistant phenotype

    DEFF Research Database (Denmark)

    Martins, Ana; Couto, Isabel; Aagaard, Lone

    2007-01-01

    Our previous studies demonstrated that exposure of a bacterium to increasing concentrations of an antibiotic would increase resistance to that antibiotic as a consequence of activating efflux pumps. This study utilises the same approach; however, it employs the methicillin-resistant Staphylococcus...... aureus (MRSA) COL strain, which is highly resistant to oxacillin (OXA). MRSA COL was adapted to 3200 mg/L of OXA. Changes in resistance to other antibiotics were evaluated and efflux pump activity during the adaptation process was determined. MRSA COL was exposed to stepwise two-fold increases of OXA....... At the end of each step, minimum inhibitory concentration determination for erythromycin (ERY) and other antibiotics was conducted. Reserpine (RES) was employed to evaluate whether resistance to ERY was dependent on efflux pump activity. Efflux pump activity was also evaluated using the ethidium bromide (EB...

  19. Carcinome à cellule vitreuse du col de l'utérus: à propos d'un cas et revue de littérature

    Science.gov (United States)

    Hakimi, Ihssane; Zazi, Abdelghani; Chahdi, Hafsa; Guelzim, Khalid; Kouach, Jaouad; Babahabib, Myabdellah; Elhassani, Myehdi; Rahali, Driss Moussaoui; Dehayni, Mohammed

    2015-01-01

    Le carcinome à cellule vitreuse du col de l'utérus est un type de histologique rare de cancer du col de l'utérus qui survient à un âge plus jeune, et s'associe au risque élevé d’échec thérapeutique et le pronostic est plus mauvais en comparaison au type cellulaire squameux. La radiothérapie est associée au risque diminué de récidive. Le but de cette étude est de récapituler à travers d'une observation et une revue de littérature les données sur l'incidence, le comportement clinique et la survie globale de patients avec le carcinome à cellule vitreuse du col de l'utérus. PMID:26664556

  20. A New COL3A1 Mutation in Ehlers-Danlos Syndrome Vascular Type With Different Phenotypes in the Same Family.

    Science.gov (United States)

    Cortini, Francesca; Marinelli, Barbara; Romi, Silvia; Seresini, Agostino; Pesatori, Angela Cecilia; Seia, Manuela; Montano, Nicola; Bassotti, Alessandra

    2017-04-01

    Vascular Ehlers-Danlos syndrome (vEDS) is a rare and severe connective tissue disorder caused by mutations in the collagen type III alpha I chain ( COL3A1) gene. We describe a pathogenetic heterozygous COL3A1 mutation c.3140 G>A, p. Gly1047Asp, identified using next-generation sequencing, in a 40-year-old Italian female. The genetic test performed on her relatives, which present different clinical phenotypes, confirmed that they carry the same mutation in heterozygous state. This finding confirms that mutations causing vEDS have an incomplete penetrance.

  1. Distribución espacial de fibroblastos endoneurales en cultivos tridimensionales en geles de colágeno Tipo I

    Directory of Open Access Journals (Sweden)

    Leslie Yaneth Leal Mejía

    2002-07-01

    Full Text Available SaludRESUMENEn la actualidad los cultivos celulares tridimensionales son muy utilizados, ya que imitan lascondiciones in vivode las células. Uno de los cultivos más desarrollados es el de fibroblastosdérmicos dentro de geles de colágeno en forma de lenteja, los cuales se contraen de un 70%a un 80% durante las primeras 24 horas de cultivo. El objetivo inicial de este trabajo fue aislarfibroblastos endoneurales (FE del nervio ciático de ratones ICR, mantenerlos en cultivo enmonocapa y luego en cultivo tridimensional en geles cilíndricos de colágeno tipo I de 1,6 mmde diámetro y 3 mm de largo, formados en las prótesis de silicona empleadas para regeneraciónde nervios periféricos; imitando las condiciones del nervio in vivopara que pueda ser emplea-dos en regeneración. Inicialmente se retira epineuro y perineuro del nervio, dejando sólo elendoneuro que se disocia con colagenasa (500U/ml y luego se le hace una disociación me-cánica. Las células libres, se cultivan en monocapa con DMEM suplementado con 20% suerobovino fetal, logrando un 95% de pureza en cultivo primario; en los subcultivos se purifican yamplifican los FE. Estos se aislan y se mezclan con una solución de colágeno tipo I a 0,5mg/mlpara la formación de los geles, teniendo en cuenta que cada gel contenga 50.000 fibroblastos,se introduce la mezcla dentro de las prótesis de silicona y se dejan gelificar, siguiendo el mismoprocedimiento se formaron geles sin células; una vez formados los geles se liberan de las cáma-ras y se cultivan en suspensión 1, 3, 5, 7, 15 y 30 días. Al cumplirse cada uno de los tiemposde cultivo, se analizó la apariencia del colágeno y la distribución de los FE en microscopíaóptica y de fluorescencia. Los cultivos primarios de FE se obtuvieron a los 5 días, lograndoacortar el tiempo de confluencia entre 15 y 20 días con respecto a lo reportado en la literatura;un dato novedoso fue la purificación total de los FE en el primer subcultivo

  2. Uso da terra e perda de solo na Bacia Hidrográfica do Rio Colônia, Bahia Land use and soil loss in the Colônia River Watershed, Bahia

    Directory of Open Access Journals (Sweden)

    Vinícius de A. Silva

    2011-03-01

    Full Text Available Mudanças no uso da terra muitas vezes potencializam a erosão hídrica acarretando perda de água, solo, nutriente e matéria orgânica dos sistemas agrícolas, razão por que se estimou a perda de solo na bacia hidrográfica do rio Colônia, na Bahia, nos últimos vinte e sete anos, utilizando-se o software SWAT (Soil and Water Assessment Tool. Para tal, procedeu-se à digitalização de mapas temáticos, interpretação de fotografias aéreas de 1975; classificação supervisionada de imagens de satélites de 2002 e produção de mapas de uso da terra. O SWAT foi utilizado na obtenção de mapas temáticos digitais por sub-bacia hidrográfica do rio Colônia, quantificação das perdas de solo em cada sub-bacia e nas formas de usos obtidos por conceito teórico, simulando as inclusões de áreas de preservação permanente, bem como mata em toda a superfície das sub-bacias. Estima-se que, entre 1975 e 2002, a média de perda de solo na bacia hidrográfica do rio Colônia foi de 47 t ha-1 ano-1 e em 2002 a estimativa de perda de solo foi de 46,64 t ha-1 ano-1. Na simulação de um cenário teórico de área de preservação permanente (APP e mata, ocorreu diminuição da média da perda de solo em toda a bacia hidrográfica do rio Colônia de, respectivamente, 9,09 t ha-1 ano-1 e 20,91 t ha-1 ano-1.Land use changes most of the time increases the hydric erosion leading to loss of water, nutrients and organic matter in agricultural systems. Thus, aiming to estimate the soil loss in the watershed of Colonia River, in Bahia, in the last twenty-seven years, the software SWAT (Soil and Water Assessment Tool was used. For the purpose, a digitalization thematic map (Arc View, interpretation of aerial photographs from 1975, supervised classification of 2002 satellite images and a land use map generation were developed. The SWAT software was used for obtaining a digital thematic map for every sub-basin of Colonia River Watershed, soil loss

  3. Natural phenomena analyses, Hanford Site, Washington

    International Nuclear Information System (INIS)

    Tallman, A.M.

    1989-01-01

    Probabilistic seismic hazard studies completed for the Washington Public Power Supply System's Nuclear Plant 2 and for the US Department of Energy's N Reactor sites, both on the Hanford Site, suggested that the Lawrence Livermore National Laboratory seismic exposure estimates were lower than appropriate, especially for sites near potential seismic sources. A probabilistic seismic hazard assessment was completed for those areas that contain process and/or waste management facilities. the lower bound magnitude of 5.0 is used in the hazard analysis and the characteristics of small-magnitude earthquakes relatively common to the Hanford Site are addressed. The recommended ground motion for high-hazard facilities is somewhat higher than the Lawrence Livermore National Laboratory model and the ground motion from small-magnitude earthquakes is addressed separately from the moderate- to large-magnitude earthquake ground motion. The severe wind and tornado hazards determined for the Hanford Siste are in agreement with work completed independently using 43 years of site data. The low-probability, high-hazard, design-basis flood at the Hanford Site is dominated by dam failure on the Columbia River. Further evaluation of the mechanisms and probabilities of such flooding is in progress. The Hanford Site is downwind from several active Cascade volcanoes. Geologic and historical data are used to estimate the ashfall hazard

  4. Spacelab ready for transport to Washington, DC

    Science.gov (United States)

    1998-01-01

    Spacelab is wrapped and ready for transport to the National Air and Space Museum in Washington, DC. Spacelab was designed by the European Space Agency (ESA) for the Space Shuttle program and first flew on STS-9 in November 1983. Its final flight was the STS-90 Neurolab mission in April 1998. A sister module will travel home and be placed on display in Europe. The Spacelab concept of modular experiment racks in a pressurized shirt-sleeve environment made it highly user-friendly and accessible. Numerous experiments conceived by hundreds of scientists on the ground were conducted by flight crews in orbit. Spacelab modules served as on-orbit homes for everything from squirrel monkeys to plant seeds. They supported astronomical as well as Earth observations, for servicing the Hubble Space Telescope and for research preparatory to the International Space Station. One of the greatest benefits afforded by the Spacelab missions was the opportunity to fly a mission more than once, with the second or third flight building on the experiences and data gathered from its predecessors.

  5. Washington in '97: Positive energy moves expected

    International Nuclear Information System (INIS)

    Matthews, C.D.

    1997-01-01

    After the stormy closing weeks of the 1996 electrons, when the fog finally lifted, a strong indication of the direction American politics is taking emerged. It is still moving clearly to the right. Donald Lambro, chief political correspondent of The Washington Times, put it this way, ''Contemporary liberalism may not be dead and buried yet, but it remains comatose and continues to wither away--without a clear and relevant agenda, without a credible champion, and without a majority party. Meanwhile, the Congress that will be sworn in next January will be one of the most conservative in decades.'' Why that will likely bode well for the US oil and gas industry is the theme of the following discussion. Outlined therein are: some thoughts on why Clinton won; a wrapup of key seats and committee members of the 105th Congress; news of a bipartisan forum for the oil/gas industry; improved outlook for movement on the Arctic National Wildlife Refuge (ANWR); progress on the national public education program; and a list of industry's challenges for the next few years

  6. Neah Bay, Washington Tsunami Forecast Grids for MOST Model

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Neah Bay, Washington Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model....

  7. Westport, Washington Tsunami Forecast Grids for MOST Model

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Westport, Washington Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model....

  8. Port Angeles, Washington Tsunami Forecast Grids for MOST Model

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Port Angeles, Washington Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST)...

  9. LiDAR (Terrain), THURSTON COUNTY, WASHINGTON, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Fugro EarthData Company furnished the collection, processing, and development of LiDAR for 825 square miles in Washington (805 square miles of Thurston County and 20...

  10. Southwestern Washington 1/3 arc-second DEM

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The 1/3-second Southwest Washington Elevation Grid provides bathymetric data in ASCII raster format of 1/3-second resolution in geographic coordinates. This grid is...

  11. Early Restoration Public Meeting, Washington, DC | NOAA Gulf Spill

    Science.gov (United States)

    benefit injured marshes, coastal dune and nearshore habitats, oysters, and human uses (on water recreation . Department of Commerce Herbert Hoover Building Auditorium 1401 Constitution Ave., NW Washington, D.C. 20230

  12. Final Report: Feasibility Study of Biomass in Snohomish County, Washington

    Energy Technology Data Exchange (ETDEWEB)

    Daryl Williams (Tulalip Tribes); Ray Clark (Clark Group)

    2005-01-31

    This report and its attachments summarizes the results of a unique tribal-farmer cooperative study to evaluate the feasibility of building one or more regional anaerobic digestion systems in Snohomish County, Washington.

  13. Toke Point, Washington Tsunami Forecast Grids for MOST Model

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Toke Point, Washington Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model....

  14. Endangered Species Case - Washington Toxics Coalition v. EPA

    Science.gov (United States)

    This Web page provides information on the Washington Toxics Coalition v. EPA case, related to protection of Pacific salmon and steelhead, and links to the biological opinions issued by the NMFS and EPA’s responses.

  15. La Push, Washington Tsunami Forecast Grids for MOST Model

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The La Push, Washington Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model....

  16. Timber resource statistics for the Olympic Peninsula, Washington.

    Science.gov (United States)

    Patricia M. Bassett; Daniel D. Oswald

    1961-01-01

    This report summarizes a 1978-79 timber resource inventory of five counties in the Olympic Peninsula of Washington: Clallam, Grays Harbor, Jefferson, Mason, and Thurston. Detailed tables of forest area, timber volume, growth, mortality, and harvest are presented.

  17. De gota em gota: violência, tempo e troca em Urabá, Colômbia

    Directory of Open Access Journals (Sweden)

    Silvia Monroy

    2014-12-01

    Full Text Available Comparo, neste texto, a modalidade de empréstimo de dinheiro imposta pelos "gota a gota" com a minha experiência como voluntária no Banco de la Esperanza,em Urabá, considerada uma das regiões mais violentas da Colômbia durante, pelo menos, quatro décadas. Mediante este contraste, passo a analisar a relação entre o que denomino "presente permanente" e determinados aspectos da troca, a saber: "o costume de ficar devendo", "a necessidade de pedir emprestado", e o imperativo de que "tudo deve ser dado". No caso estudado, tais efeitos são aspectos primordiais da vida coletiva e da reprodução social. Busco, em particular, explorar a relação entre violência e "economia", vínculo que costuma ser relegado ao campo da ilegalidade.

  18. Uso da terra e perda de solo na Bacia Hidrográfica do Rio Colônia, Bahia

    OpenAIRE

    Silva,Vinícius de A.; Moreau,Mauricio S.; Moreau,Ana M. S. dos S.; Rego,Neylor A. C.

    2011-01-01

    Mudanças no uso da terra muitas vezes potencializam a erosão hídrica acarretando perda de água, solo, nutriente e matéria orgânica dos sistemas agrícolas, razão por que se estimou a perda de solo na bacia hidrográfica do rio Colônia, na Bahia, nos últimos vinte e sete anos, utilizando-se o software SWAT (Soil and Water Assessment Tool). Para tal, procedeu-se à digitalização de mapas temáticos, interpretação de fotografias aéreas de 1975; classificação supervisionada de imagens de satélites de...

  19. Acesso endoscópico transepto-interforniceal para cistos colóides: relato de caso

    Directory of Open Access Journals (Sweden)

    VIALOGO JOSUÉ GUIMARÃES GRANHA

    2000-01-01

    Full Text Available A abordagem endoscópica de cistos colóides ainda é matéria controversa. As opções de tratamento são variadas: observação com exames seriados, tratamento da hidrocefalia, aspiração por estereotaxia, microcirurgia e endoscopia. O autor relata o caso de um paciente com cisto colóide do terceiro ventrículo, localizado em posição retroforaminal, no teto diencefálico, dissecando os dois fornices projetando-se supero-posteriormente em direção ao assoalho do ventrículo lateral, por dentro do cavum do septo pelúcido. A lesão foi alcançada com neuroendoscópio rígido, através de um orifício de trépano pré-coronal situado a 2,5 cm da linha média (ponto de Kocher. Seguindo por via transventricular, a lâmina direita do septo pelúcido foi aberta, posterior à veia septal. A lesão, muito endurecida, situada entre os dois fórnices, foi parcialmente ressecada através da via endoscópica transventricular transepto-interforniceal. Realizamos também septostomia e terceiro ventriculostomia endoscópicas neste paciente. Os diferentes tipos de procedimentos cirúrgicos das patologias situadas no terceiro ventrículo são discutidos com ênfase no papel da endoscopia.

  20. Distribuciones simuladas de valores de d13c de colágeno humano: implicancias para los estudios paleodietarios

    Directory of Open Access Journals (Sweden)

    Barrientos, Gustavo

    2005-01-01

    Full Text Available El objetivo de este trabajo es presentar los primeros resultados derivados de la aplicación de un programa de simulación de distribuciones de valores de δ13C de colágeno humano (DeltaCarb130.0, desarrollado por los autores. DeltaCarb130.0 es un programa simple que simula la composición isotópica (δ13C del colágeno de huesos humanos obtenida a través de una dieta de composición variable, constituida por tres ingestas diarias, durante un período de 1825 días (5 años. La composición de la dieta y los valores de δ13C de cada recurso dietario (vegetales C3 y C4, carne de herbívoros consumidores de C3 y C4, de mamíferos marinos, de peces y moluscos se obtienen, en cada etapa de la simulación, a través de un proceso aleatorio a partir de datos de abundancia relativa y rangos de valores de δ13C de cada tipo de recurso. Se analizarán estadísticamente las distribuciones de valores de δ13C obtenidas a partir de n corridas del programa para cada combinación dietaria, con la finalidad de obtener medidas de tendencia central y de dispersión asociadas a cada tipo de dieta y calcular la probabilidad de obtener valores "outliers", un rasgo frecuente en el estudio de casos arqueológicos.

  1. Prácticas escolares en el Colégio Americano: un enfoque en la cultura material

    Directory of Open Access Journals (Sweden)

    Marlucy do Socorro Aragão de Sousa

    2017-05-01

    Full Text Available Este artículo aborda las prácticas escolares y la cultura material de la escuela en Belém do Pará, a finales del siglo XIX. El objetivo es analizar cómo se configuraron la cultura material y las prácticas escolares en el Colégio Americano, ideado por José Veríssimo entre 1884 y 1890. Se usa como fuente la obra Noticia Geral sobre o Collegio Americano. Se utilizó la metodología histórico-hermenéutica. Al analizar la cultura material de la escuela, se tiene como horizonte la percepción de sus vínculos con el contexto histórico, cultural y social en el que se generó un nuevo modelo de educación que destacaba la civilidad, la nación y la moral. Por consiguiente, dichos artefactos no eran simples objetos, sino que revelaron las relaciones conflictivas o pacíficas de aquel contexto, e incluso las prácticas escolares de la institución. Se observa que el uso y la descripción de los objetos dispuestos en la estructura del Colégio Americano, para su creador, tenían el objetivo de establecer y mantener normas que debían formar hombres disciplinados, que se vincularan directamente con el propósito de la educación física, moral y cívica de los estudiantes.

  2. 77 FR 51564 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2012-08-24

    ... Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington... of human remains under the control of the Thomas Burke Memorial Washington State Museum (Burke Museum...

  3. Washington Windplant No. 1: Botanical resources field survey. Appendix B to Washington Windplant No. 1 EIS

    International Nuclear Information System (INIS)

    1994-12-01

    KENETECH Windpower, Inc., has applied to Klickitat County for a conditional use permit to construct and operate a 115 Megawatt windfarm on an approximately 5110 hectare (12,630 acre) site in the Columbia Hills near Goldendale, Washington. A transmission services agreement between the Bonneville Power Administration (BPA) and utilities purchasing the Project's output will also be required. Klickitat County and BPA initiated the preparation of a joint SEPA/NEPA Environmental Impact Statement, under the authority of the Washington State Environmental Policy Act and the National Environmental Policy Act. The EIS addresses, among other issues, the Project's potential impact on rare, threatened or endangered, and sensitive plant species and communities as well as plant species of potential cultural importance. A field survey along potential impact corridors (turbine strings, roadways, and the transmission line alignment) was conducted between April and June, 1994 in order to identify rare plant species, high-quality native plant communities, and plant species of potential cultural importance present in these corridors. In addition, habitat maps of the entire 5110-ha project area were field verified. This report contains the results of that survey and an assessment of the potential project impacts

  4. Nonproliferation Education at the University of Washington

    International Nuclear Information System (INIS)

    Jones, Chris D.; Leek, K.M.

    2006-01-01

    The nonproliferation curriculum at the University of Washington (UW) is the product of collaboration between Pacific Northwest Center for Global Security (PNWCGS) at the Pacific Northwest National Laboratory (PNNL) and the Jackson School of International Studies (JSIS) and Department of Political Science at the University of Washington. This collaboration began in 2001 with the establishment the Institute for Global and Regional Security Studies (IGRSS). IGRSS is housed in the Jackson School, which will celebrate its centenary in 2008 as a center for the study of world regions. PNNL also engages in a number of collaborative relationships with UW units in the natural and applied sciences. The principal goal of IGRSS has been to develop courses that draw graduates and undergraduates into careers in the field of nonproliferation. Since offering its first courses in 2002, IGRSS has assisted a substantial number of UW graduate students in submitting successful applications for nonproliferation positions in U.S. government agencies, including the Nonproliferation Graduate Program at the National Nuclear Security Administration. Since 2001, several UW undergraduates have begun careers in the field of nonproliferation, either by working at national laboratories or enrolling in non-UW graduate programs. The UW brought to its nonproliferation partnership with PNNL long-established programs in a wide range of professional programs and academic disciplines, including the 14 interdisciplinary regional and topical programs of the Jackson School of International Studies. The JSIS is an interdisciplinary and interdepartmental enterprise that brings together faculty and students from across the UW. Since the late 1940s the UW has trained experts for the nation's foreign policy community in programs focused in the languages, cultures, and histories of regions deemed critical to U.S. national security. However, since the termination of its program in nuclear engineering several

  5. Uranium concentrations in groundwater, northeastern Washington

    Science.gov (United States)

    Kahle, Sue C.; Welch, Wendy B.; Tecca, Alison E.; Eliason, Devin M.

    2018-04-18

    A study of uranium in groundwater in northeastern Washington was conducted to make a preliminary assessment of naturally occurring uranium in groundwater relying on existing information and limited reconnaissance sampling. Naturally occurring uranium is associated with granitic and metasedimentary rocks, as well as younger sedimentary deposits, that occur in this region. The occurrence and distribution of uranium in groundwater is poorly understood. U.S. Environmental Protection Agency (EPA) regulates uranium in Group A community water systems at a maximum contaminant level (MCL) of 30 μg/L in order to reduce uranium exposure, protect from toxic kidney effects of uranium, and reduce the risk of cancer. However, most existing private wells in the study area, generally for single family use, have not been sampled for uranium. This document presents available uranium concentration data from throughout a multi-county region, identifies data gaps, and suggests further study aimed at understanding the occurrence of uranium in groundwater.The study encompasses about 13,000 square miles (mi2) in the northeastern part of Washington with a 2010 population of about 563,000. Other than the City of Spokane, most of the study area is rural with small towns interspersed throughout the region. The study area also includes three Indian Reservations with small towns and scattered population. The area has a history of uranium exploration and mining, with two inactive uranium mines on the Spokane Indian Reservation and one smaller inactive mine on the outskirts of Spokane. Historical (1977–2016) uranium in groundwater concentration data were used to describe and illustrate the general occurrence and distribution of uranium in groundwater, as well as to identify data deficiencies. Uranium concentrations were detected at greater than 1 microgram per liter (μg/L) in 60 percent of the 2,382 historical samples (from wells and springs). Uranium concentrations ranged from less than 1 to

  6. National Uranium Resource Evaluation: Okanogan Quadrangle, Washington

    International Nuclear Information System (INIS)

    Bernardi, M.L.; Powell, L.K.; Wicklund, M.A.

    1982-06-01

    The Okanogan Quadrangle, Washington, was evaluated to identify and delineate areas containing environments favorable for the occurrence of uranium deposits using criteria developed for the National Uranium Resource Evaluation program. Reconnaissance and detailed surface studies were augmented by aerial radiometric surveys and hydrogeochemical and stream-sediment reconnaissance studies. The results of the investigations indicate six environments favorable for uranium deposits. They are unclassified, anatectic, allogenic, and contact-metasomatic deposits in Late Precambrian and (or) Early Paleozoic mantling metamorphic core-complex rocks of the Kettle gneiss dome; magmatic-hydrothermal deposits in the Gold Creek pluton, the Magee Creek pluton, the Wellington Peak pluton, and the Midnite Mine pluton, all located in the southeast quadrant of the quadrangle; magmatic-hydrothermal allogenic deposits in Late Paleozoic and (or) Early Mesozoic black shales in the Castle Mountain area; allogenic deposits in Early Paleozoic metasedimentary rocks in the Harvey Creek area and in Late Precambrian metasedimentary rocks in the Blue Mountain area; and sandstone deposits in Eocene sedimentary rocks possibly present in the Enterprise Valley. Seven geologic units are considered unfavorable for uranium deposits. They are all the remaining metamorphic core-complex rocks, Precambrian metasedimentary rocks,Tertiary sedimentary and volcanic rocks, and all Pleistocene and Recent deposits; and, excluding those rocks in the unevaluated areas, include all the remaining plutonic rocks, Paleozoic miogeoclinical rocks, and Upper Paleozoic and Mesozoic eugeosynclinal rocks. Three areas, the Cobey Creek-Frosty Creek area, the Oregon City Ridge-Wilmont Creek area, and the area underlain by the Middle Cambrian Metaline Formation and its stratigraphic equivalents may possibly be favorable but are unevaluated due to lack of data

  7. Modeling landslide recurrence in Seattle, Washington, USA

    Science.gov (United States)

    Salciarini, Diana; Godt, Jonathan W.; Savage, William Z.; Baum, Rex L.; Conversini, Pietro

    2008-01-01

    To manage the hazard associated with shallow landslides, decision makers need an understanding of where and when landslides may occur. A variety of approaches have been used to estimate the hazard from shallow, rainfall-triggered landslides, such as empirical rainfall threshold methods or probabilistic methods based on historical records. The wide availability of Geographic Information Systems (GIS) and digital topographic data has led to the development of analytic methods for landslide hazard estimation that couple steady-state hydrological models with slope stability calculations. Because these methods typically neglect the transient effects of infiltration on slope stability, results cannot be linked with historical or forecasted rainfall sequences. Estimates of the frequency of conditions likely to cause landslides are critical for quantitative risk and hazard assessments. We present results to demonstrate how a transient infiltration model coupled with an infinite slope stability calculation may be used to assess shallow landslide frequency in the City of Seattle, Washington, USA. A module called CRF (Critical RainFall) for estimating deterministic rainfall thresholds has been integrated in the TRIGRS (Transient Rainfall Infiltration and Grid-based Slope-Stability) model that combines a transient, one-dimensional analytic solution for pore-pressure response to rainfall infiltration with an infinite slope stability calculation. Input data for the extended model include topographic slope, colluvial thickness, initial water-table depth, material properties, and rainfall durations. This approach is combined with a statistical treatment of rainfall using a GEV (General Extreme Value) probabilistic distribution to produce maps showing the shallow landslide recurrence induced, on a spatially distributed basis, as a function of rainfall duration and hillslope characteristics.

  8. Natural gas pipeline leaks across Washington, DC.

    Science.gov (United States)

    Jackson, Robert B; Down, Adrian; Phillips, Nathan G; Ackley, Robert C; Cook, Charles W; Plata, Desiree L; Zhao, Kaiguang

    2014-01-01

    Pipeline safety in the United States has increased in recent decades, but incidents involving natural gas pipelines still cause an average of 17 fatalities and $133 M in property damage annually. Natural gas leaks are also the largest anthropogenic source of the greenhouse gas methane (CH4) in the U.S. To reduce pipeline leakage and increase consumer safety, we deployed a Picarro G2301 Cavity Ring-Down Spectrometer in a car, mapping 5893 natural gas leaks (2.5 to 88.6 ppm CH4) across 1500 road miles of Washington, DC. The δ(13)C-isotopic signatures of the methane (-38.2‰ ± 3.9‰ s.d.) and ethane (-36.5 ± 1.1 s.d.) and the CH4:C2H6 ratios (25.5 ± 8.9 s.d.) closely matched the pipeline gas (-39.0‰ and -36.2‰ for methane and ethane; 19.0 for CH4/C2H6). Emissions from four street leaks ranged from 9200 to 38,200 L CH4 day(-1) each, comparable to natural gas used by 1.7 to 7.0 homes, respectively. At 19 tested locations, 12 potentially explosive (Grade 1) methane concentrations of 50,000 to 500,000 ppm were detected in manholes. Financial incentives and targeted programs among companies, public utility commissions, and scientists to reduce leaks and replace old cast-iron pipes will improve consumer safety and air quality, save money, and lower greenhouse gas emissions.

  9. Nuclear waste - A view from Washington, DC

    International Nuclear Information System (INIS)

    Mc Cormack, M.

    1984-01-01

    The view from Washington is an optimistic one. With support of the vast amount of research, development and engineering that scientists and engineers have completed and will do in the future, this country certainly is now headed towards a successful and orderly program for handling all radioactive wastes safely and inexpensively. The high-level waste program, for instance, will require only about 2% of the cost of electricity produced from nuclear power. It is the need for the federal government to create, by law, a public corporation for handling the fuel cycle for commercial nuclear fuel. Such a Federal Nuclear Fuel Cycle Corporation (FNFCC) would handle almost all of the nuclear power fuel cycle in a manner similar to the DOE management of the fuel cycle for the weapons program. Except for the mining and milling of uranium and the fabrication of uranium (only) fuel elements, the federal government would pre-empt ownership of all facilities used in the fuel cycle and operate them by contract with private industry (as with the weapons fuel cycle). Ownership of all fissile and fertile material (and all existing and future fuel elements) would be preempted by law and vested in the FNFCC. Reprocessing fuel to extract and glassify waste for permanent geologic disposal is the most attractive method for handling spent fuel from a safety and environmental perspective. Also recycling fuel is probably more environmentally attractive than mining more uranium, especially from lower grade ores. This concept of an FNFCC has been suggested to the Administration and to some Congressional leaders. There has been no known opposition expressed, but there is some indication of a reluctance to undertake such a major problem-solving initiative in one step

  10. THE PROPOSED NATIONAL ARBORETUM AT WASHINGTON.

    Science.gov (United States)

    Coville, F V

    1925-12-25

    Nature. The proposed national arboretum at Washington would contain a permanent living collection of trees and other outdoor plants for purposes of scientific research and education. It would include the trees, shrubs, and perennials used in forestry and horticulture, and the wild relatives of these plants. It would be a bureau of standards for horticulture. It would contain a water garden and a wildrice preserve, and it would serve incidentally as a bird sanctuary. Economic value. The arboretum would make the work of the Department of Agriculture more valuable to the country in many ways, but especially through plant breeding. The development of faster-growing timber trees, improved fruits, and disease-resistant plants generally, through the facilities afforded by the arboretum, would increase profoundly the agricultural wealth and welfare of the United States. Location. The Mount Hamilton and Hickey Hill tracts in the District of Columbia, together with the Anacostia River flats above Benning Bridge, constitute an admirable site for the arboretum, convenient in location and with a great variety of soils. Cost. About 400 acres of the proposed site is already owned by the government. It consists of marsh land, about to be drained by army engineers. The Mount Hamilton and Hickey Hill area, 408 acres, privately owned, was reported by the assessor in January, 1925, to be valued at $343,048, distributed among thirty owners. Maintenance. If the purchase of the Mount Hamilton and Hickey Hill tracts in the fiscal year 1927 is authorized, it is estimated that the cost of maintenance of the arboretum for the first three years would be as follows: 1927. Nothing 1928. $25,000 1929. $50,000.

  11. Horizontal gene transfer of a ColV plasmid has resulted in a dominant avian clonal type of Salmonella enterica serovar Kentucky.

    Directory of Open Access Journals (Sweden)

    Timothy J Johnson

    Full Text Available Salmonella enterica continues to be a significant cause of foodborne gastrointestinal illness in humans. A wide variety of Salmonella serovars have been isolated from production birds and from retail poultry meat. Recently, though, S. enterica subsp. enterica serovar Kentucky has emerged as one of the prominent Salmonella serovars isolated from broiler chickens. Recent work suggests that its emergence apparently coincides with its acquisition of a ColV virulence plasmid. In the present study, we examined 902 Salmonella isolates belonging to 59 different serovars for the presence of this plasmid. Of the serovars examined, the ColV plasmid was found only among isolates belonging to the serovars Kentucky (72.9%, Typhimurium (15.0% and Heidelberg (1.7%. We demonstrated that a single PFGE clonal type of S. Kentucky harbors this plasmid, and acquisition of this plasmid by S. Kentucky significantly increased its ability to colonize the chicken cecum and cause extraintestinal disease. Comparison of the completed sequences of three ColV plasmids from S. Kentucky isolated from different geographical locales, timepoints and sources revealed a nearly identical genetic structure with few single nucleotide changes or insertions/deletions. Overall, it appears that the ColV plasmid was recently acquired by a single clonal type S. Kentucky and confers to its host enhanced colonization and fitness capabilities. Thus, the potential for horizontal gene transfer of virulence and fitness factors to Salmonella from other enteric bacteria exists in poultry, representing a potential human health hazard.

  12. Lethal chondrodysplasia in a family of Holstein cattle is associated with a de novo splice site variant of COL2A1

    DEFF Research Database (Denmark)

    Agerholm, Jørgen Steen; Menzi, Fiona; McEvoy, Fintan

    2016-01-01

    was predicted to affect splicing as it altered the conserved splice donor sequence GT at the 5’-end of COL2A1 intron 36, which was changed to AT. All five available cases carried the mutant allele in heterozygous state and all five dams were homozygous wild type. The sire VH Cadiz Captivo was shown...

  13. Produção de identidades nos trabalhos apresentados em colóquios luso-brasileiros de 2002 e 2004

    Directory of Open Access Journals (Sweden)

    Leonardo Dias Ferreira

    2017-12-01

    Full Text Available O presente artigo trata-se do estudo da internacionalização do campo do currículo com enfoque em trabalhos apresentados em colóquios luso-brasileiros de 2002 e 2004 sobre questões curriculares e produção de identidades. Para isso, faremos uma contextualização histórica do colóquio luso-brasileiro, depois refletiremos sobre a internacionalização do campo do currículo e a visão desta concepção presente nos editoriais dos colóquios Luso-Brasileiros e na última seção falaremos sobre a categoria identidade. Concluiu-se que  entender a relação direta de questões sobre multiculturalidade e formação da identidade do sujeito envolvido no processo de ensino-aprendizagem escolar é essencial para que a escola seja voltada para formação e interação social, de tal modo, que os alunos possam compartilhar suas crenças e valores e aprender a respeitar, reconhecer e valorizar as diferenças do outro. Palavras Chaves: internacionalização do campo do currículo; colóquios luso-brasileiros, identidade.

  14. COL1A2 gene analysis in a Czech osteogenesis imperfecta patient: a candidate novel mutation in a patient affected by osteogenesis imperfecta type 3

    Directory of Open Access Journals (Sweden)

    Hrušková L

    2015-08-01

    Full Text Available Lucie Hrušková,1 Ivo Mařík,2,3 Stella Mazurová,1 Pavel Martásek,1 Ivan Mazura1 1Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic; 2Ambulant Centre for Defects of Locomotor Apparatus 1.1.c., Prague, Czech Republic; 3Faculty of Medical Studies, West Bohemia University, Pilsen, Czech RepublicAbstract: Osteogenesis imperfecta is a heritable bone fragility disease with a heterogenic genetic origin. Most cases result from mutations of either the COL1A1 gene or the COL1A2 gene. We identified a novel COL1A2 gene mutation in a Czech patient, born to unaffected parents, who was diagnosed according to clinical and anthropometric findings and radiographic features as having type 3 osteogenesis imperfecta, which is a severe form of this disease. The identified Gly814Trp mutation was predicted by a number of complementary bioinformatic programs to result in functional alteration of the protein. This case report provides both evidence of a novel COL1A2 mutation resulting in type 3 osteogenesis imperfecta and a genotype:phenotype correlation in this affected individual. Keywords: osteogenesis imperfecta type 3, collagen, alpha-2 (I chain, substitution, sequencing 

  15. Somatic mosaicism for the COL7A1 mutation p.Gly2034Arg in the unaffected mother of a patient with dystrophic epidermolysis bullosa pruriginosa

    NARCIS (Netherlands)

    van den Akker, P. C.; Pasmooij, A. M. G.; Meijer, R.; Scheffer, H.; Jonkman, M. F.

    Dystrophic epidermolysis bullosa (DEB) is a heritable blistering disorder caused by mutations in the type VII collagen gene, COL7A1. Although revertant mosaicism is well known in DEB, 'forward' somatic mosaicism, in which a pathogenic mutation arises on a wild-type (WT) background, extending beyond

  16. Somatic mosaicism for the COL7A1 mutation p.Gly2034Arg in the unaffected mother of a patient with dystrophic epidermolysis bullosa pruriginosa

    NARCIS (Netherlands)

    Akker, P.C. van den; Pasmooij, A.M.; Meijer, R.; Scheffer, H.; Jonkman, M.F.

    2015-01-01

    Dystrophic epidermolysis bullosa (DEB) is a heritable blistering disorder caused by mutations in the type VII collagen gene, COL7A1. Although revertant mosaicism is well known in DEB, 'forward' somatic mosaicism, in which a pathogenic mutation arises on a wild-type (WT) background, extending beyond

  17. Influence of tra genes of IncP and F plasmids on the mobilization of small Kanamycin resistance ColE1-Like plasmids in bacterial biofilms

    Science.gov (United States)

    Background: Horizontal gene transfer is a mechanism for movement of antibiotic resistance genes among bacteria. Some small kanamycin resistance (KanR) ColE1-like plasmids isolated from different serotypes of Salmonella enterica were shown to carry mobilization genes; although not self-transmissibl...

  18. A novel splice variant in the N-propeptide of COL5A1 causes an EDS phenotype with severe kyphoscoliosis and eye involvement.

    Directory of Open Access Journals (Sweden)

    Sofie Symoens

    Full Text Available BACKGROUND: The Ehlers-Danlos Syndrome (EDS is a heritable connective tissue disorder characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. The classic subtype of EDS is caused by mutations in one of the type V collagen genes (COL5A1 and COL5A2. Most mutations affect the type V collagen helical domain and lead to a diminished or structurally abnormal type V collagen protein. Remarkably, only two mutations were reported to affect the extended, highly conserved N-propeptide domain, which plays an important role in the regulation of the heterotypic collagen fibril diameter. We identified a novel COL5A1 N-propeptide mutation, resulting in an unusual but severe classic EDS phenotype and a remarkable splicing outcome. METHODOLOGY/PRINCIPAL FINDINGS: We identified a novel COL5A1 N-propeptide acceptor-splice site mutation (IVS6-2A>G, NM_000093.3_c.925-2A>G in a patient with cutaneous features of EDS, severe progressive scoliosis and eye involvement. Two mutant transcripts were identified, one with an exon 7 skip and one in which exon 7 and the upstream exon 6 are deleted. Both transcripts are expressed and secreted into the extracellular matrix, where they can participate in and perturb collagen fibrillogenesis, as illustrated by the presence of dermal collagen cauliflowers. Determination of the order of intron removal and computational analysis showed that simultaneous skipping of exons 6 and 7 is due to the combined effect of delayed splicing of intron 7, altered pre-mRNA secondary structure, low splice site strength and possibly disturbed binding of splicing factors. CONCLUSIONS/SIGNIFICANCE: We report a novel COL5A1 N-propeptide acceptor-splice site mutation in intron 6, which not only affects splicing of the adjacent exon 7, but also causes a splicing error of the upstream exon 6. Our findings add further insights into the COL5A1 splicing order and show for the first time that a single COL5A1 acceptor-splice site

  19. The Variants Within the COL5A1 Gene are Associated with Reduced Risk of Anterior Cruciate Ligament Injury in Skiers

    Directory of Open Access Journals (Sweden)

    Stępień-Słodkowska Marta

    2015-03-01

    Full Text Available The purpose of this study was to examine the association of the BstUI RFLP C/T (rs 12722 and DpnII RFLP C/T (rs 13946 COL5A1 polymorphisms, individually and as haplotypes, with anterior cruciate ligament ruptures in recreational skiers. Subjects were 138 male recreational skiers with surgically diagnosed primary anterior cruciate ligament ruptures. The control group consisted of 183 apparently healthy male recreational skiers, who were without any self-reported history of ligament or tendon injury. DNA was extracted from buccal cells donated by the subjects and genotyping was carried out using real-time PCR. The genotype distributions for both polymorphisms met Hardy- Weinberg expectations in both groups. There were no significant differences in genotype distribution of allele frequencies of COL5A1 BstUI RFLP C/T and COL5A1 DpnII RFLP C/T polymorphisms between the ACL rupture and control groups. The T-T (BstUI RFLP T, DpnII RFLP T haplotype was the most common (55.6%. The haplotype T-C was not present in any of the subjects. There was an underrepresentation tendency of the C-T haplotype in the study group compared to controls under recessive mode of inheritance. Higher frequency of the COL5A1 BstUI RFLP C/T and COL5A1DpnII RFLP C/T polymorphisms haplotype is associated with reduced risk of anterior cruciate ligament injury in a group of apparently healthy male recreational skiers.

  20. Analysis of four families with the Stickler syndrome by linkage studies. Identification of a new premature stop codon in the COL2A1 gene in a family

    Energy Technology Data Exchange (ETDEWEB)

    Bonaventure, J.; Lasselin, C. [Hopital Necker, Paris (France); Toutain, A. [CHU Bretonneau, Tours (France)] [and others

    1994-09-01

    The Stickler syndrome is an arthro-ophthalmopathy which associates progressive myopia with vitreal degeneration and retinal detachment. Cleft palate, cranio-facial abnormalities, deafness and osteoarthritis are often associated symptoms. Genetic heterogeneity of this autosomal dominant disease was consistent with its large clinical variability. Linkage studies have provided evidence for cosegregation of the disease with COL2A1, the gene coding for type II collagen, in about 50% of the families. Four additional families are reported here. Linkage analyses by using a VNTR located in the 3{prime} region of the gene were achieved. In three families, positive lod scores were obtained with a cumulative maximal value of 3.5 at a recombination fraction of 0. In one of these families, single strand conformation analysis of 25 exons disclosed a new mutation in exon 42. Codon for glutamic acid at position a1-803 was converted into a stop codon. The mutation was detected in DNA samples from all the affected members of the family but not in the unaffected. This result confirms that most of the Stickler syndromes linked to COL2A1 are due to premature stop codons. In a second family, an abnormal SSCP pattern of exon 34 was detected in all the affected individuals. The mutation is likely to correspond to a splicing defect in the acceptor site of intron 33. In one family the disease did not segregate with the COL2A1 locus. Further linkage studies with intragenic dimorphic sites in the COL10A1 gene and highly polymorphic markers close to the COL9A1 locus indicated that this disorder did not result from defects in these two genes.

  1. Role of CTA1R7K-COL-DD as a novel therapeutic mucosal tolerance-inducing vector for treatment of collagen-induced arthritis.

    Science.gov (United States)

    Hasselberg, Annemarie; Schön, Karin; Tarkowski, Andrej; Lycke, Nils

    2009-06-01

    To determine whether a cholera toxin-derived, novel immunomodulating fusion protein, CTA1R7K-COL-DD, carrying the class II major histocompatibility complex H-2q-restricted type II collagen peptide aa 259-274, can induce therapeutic tolerance and prevent collagen-induced arthritis (CIA) when administered intranasally in DBA/1 mice, and to assess whether ADP-ribosylation at the mucosal membranes exerts a regulatory function such that the outcome of tolerance or immune enhancement can be controlled. DBA/1 mice with CIA were treated intranasally with CTA1R7K-COL-DD. The therapeutic effect was monitored for 46 days after the onset of disease. Clinical scoring of disease, histologic examination of inflammation, and bone erosion were assessed, and cytokine levels were determined in the serum or supernatants from splenocytes stimulated with recall antigen. The protective effect of CTA1R7K-COL-DD resulted in roughly 60% of the mice having no clinical signs or histologic evidence of disease after treatment, and those with CIA had significantly milder disease with less bone erosion. The protective status was associated with lower serum titers of IgG1, IgG2a, IgG2b, and IgG3 anticollagen and a substantial decrease in the production of interleukin-6 (IL-6), IL-17, and interferon-gamma, while levels of IL-10 were markedly up-regulated both in the serum and at the T cell level. The enzymatically inactive mutant fusion protein CTA1R7K-COL-DD provided substantial therapeutic protection against CIA following intranasal administration. The mechanism behind the effect appears to be mediated by peptide-specific regulatory T cells induced by mucosal exposure to the peptide containing CTA1R7K-COL-DD vector. In addition, ADP-ribosylation at the mucosal membranes acts as a key regulator controlling mucosal tolerance or immunity.

  2. Refining aging criteria for northern sea otters in Washington State

    Science.gov (United States)

    Schuler, Krysten L.; Baker, Bridget B.; Mayer, Karl A.; Perez-Heydrich, Carolina; Holahan, Paula M.; Thomas, Nancy J.; White, C. LeAnn

    2018-01-01

    Measurement of skull ossification patterns is a standard method for aging various mammalian species and has been used to age Russian, Californian, and Alaskan sea otter populations. Cementum annuli counts have also been verified as an accurate aging method for the Alaskan sea otter population. In this study, cementum annuli count results and skull ossification patterns were compared as methods for aging the northern sea otter (Enhydra lutris kenyoni) population in Washington State. Significant agreement was found between the two methods suggesting that either method could be used to age the Washington population of otters. This study also found that ossification of the squamosal-jugal suture at the ventral glenoid fossa can be used to differentiate male subadults from adults. To assist field biologists or others without access to cementum annuli or skull ossification analysis techniques, a suite of morphologic, physiologic, and developmental characteristics were analyzed to assess whether a set of these more easily accessible parameters could also predict age class for the Washington population of otters. Tooth condition score, evidence of reproductive activity in females, and tooth eruption pattern were identified as the most useful criteria for classifying Washington sea otters as pups, juveniles, subadults, or adults/aged adults. A simple decision tree based on characteristics accessible in the field or at necropsy was created that can be used to reliably predict age class of Washington sea otters as determined by cementum annuli.

  3. Adição de colágeno em pão sem glúten elaborado com farinha de arroz

    Directory of Open Access Journals (Sweden)

    Carolina da Silva Graça

    2017-02-01

    Full Text Available Resumo Os portadores da doença celíaca, uma enteropatia, apresentam intolerância ao glúten e a restrição de alimentos que o contenham mostra-se eficaz na normalização da função intestinal e das manifestações clínicas. O objetivo do presente estudo foi avaliar o efeito da adição de colágeno em pó e em fibra nas características estruturais, tecnológicas e sensoriais de pães sem glúten, elaborados com farinha de arroz. Primeiramente, foram realizadas adições de 2%, 4%, 6% e 8% de colágeno, sob as formas em pó e em fibra, em pães elaborados com farinha de arroz, sendo estes avaliados quanto às suas características tecnológicas: volume específico, firmeza do miolo e cor da crosta e do miolo. O pão que apresentou as melhores características tecnológicas foi caracterizado quanto à sua composição química (proteínas, lipídios, cinzas, umidade e carboidratos, avaliado quanto às perdas decorrentes da cocção e submetido à análise sensorial. As massas cruas dos pães adicionados com o colágeno em fibra, assim como a massa do pão padrão (sem adição de colágeno, foram avaliadas através de reologia e microscopia eletrônica de varredura. A adição de colágeno aos pães elaborados com farinha de arroz não colaborou para a formação de uma rede semelhante à formada pelo glúten. O pão da formulação com 4% de colágeno em fibra apresentou as características tecnológicas mais próximas ao esperado, não diferindo significativamente quanto à composição química, do pão padrão. Por meio da análise sensorial, constatou-se que os pães sem glúten (padrão e com 4% de colágeno em fibra obtiveram aceitação sensorial superior a 75%. Conclui-se, com o estudo, que é possível adicionar colágeno em pães sem glúten, sendo que o pão adicionado de 4% de colágeno em fibra apresentou características mais próximas às do pão padrão testado.

  4. Participation des médecins généralistes de la province de Benimellal (Maroc) dans le dépistage du cancer du col

    Science.gov (United States)

    Nani, Samira; Benallal, Mohamed; Hassoune, Samira; Kissi, Dounia; Maaroufi, Abderrahmane

    2013-01-01

    Introduction Au Maroc, chaque année il y aurait environ 2000 nouveaux cas de cancer du col et les 2/3 des cas sont pris en charge à un stade très avancé. Nous avons mené une étude transversale, exhaustive incluant les 71 médecins généralistes exerçant dans les établissements de soins de santé de base du secteur public et privé de la province de Benimellal. Le but était d’évaluer leurs connaissances et leur participation au dépistage du cancer du col. Méthodes Nous avons mené une étude transversale, exhaustive incluant les 71 médecins généralistes exerçant dans les établissements de soins de santé de base du secteur public et privé de la province de Benimellal. Le but était d’évaluer leurs connaissances et leur participation au dépistage du cancer du col. Résultats Le niveau de connaissance était relativement modeste, 22 médecins généraliste avaient répondu à la question sur l'incidence du cancer du col au Maroc, Parmi eux (81,8%) avaient donné une réponse incorrecte. L'Herpes Papilloma virus comme facteur de risque du cancer du col a été identifié par seulement 21% des médecins généralistes. La participation au dépistage était également défaillante, 92,8% n'avaient jamais pratiqué le FCV chez leurs patientes à cause principalement du manque de formation (95,5%). Conclusion Les résultats montrent la nécessité d'améliorer les connaissances théoriques et pratique des médecins généralistes concernant le dépistage du cancer du col. PMID:23785557

  5. Rapid Crustal Uplift at Birch Bay, Washington

    Science.gov (United States)

    Sherrod, B. L.; Kelsey, H. M.; Blakely, R. J.

    2010-12-01

    Geomorphology and coastal marsh stratigraphy suggest late Holocene uplift of the shoreline at Birch Bay, located northwest of Bellingham, Washington, during an earthquake on a shallow fault. LiDAR images show a raised, late Holocene shoreline along Birch Bay, with ~1 m of elevation difference between the modern shoreline and the inferred paleoshoreline. Commercial seismic reflection images reveal an anticline in Tertiary and possibly Quaternary deposits underlying Birch Bay. NW-trending magnetic anomalies are likely associated with the Birch Bay anticline and other nearby structures. Taken together, the geophysical data and lidar images suggest uplift of young deposits along a NW-trending blind reverse fault. Stratigraphy from Terrell Creek marsh, located just south of Birch Bay, shows freshwater peat buried by lower intertidal muds, indicating local submergence ~1300 yr BP. Stratigraphy of a 70-cm sediment core from Birch Bay marsh, sitting astride the anticline imaged with seismic reflection data, shows mud buried by detrital peat. One radiocarbon age from the core places the abrupt change from mud to peat prior to 1520-1700 yr BP. We divide fossil diatom assemblages straddling the mud-peat contact at Birch Bay into three zones. The oldest zone consists primarily of intertidal and marine diatoms, dominated by Paralia sulcata, Scoleoneis tumida, Grammataphora oceanica, and Gyrosigma balticum. An intermediate zone, beginning at the sharp contact between mud and overlying peat, consists of a mixture of brackish marsh and freshwater species, dominated by Diploneis interrupta, with lesser amounts of Aulacoseira sp., Pinnularia viridis, Eunotia pectinalis, and Paralia sulcata. A third and youngest zone lies in the upper half of the peat and is dominated by poorly preserved freshwater diatoms, mostly Aulacoseira cf. crassapuntata, Pinnularia viridis, P. maior, Eunotia pectinalis, and E. praerupta. Paleoecological inferences, based on distributions of modern diatoms

  6. Thatcher Bay, Washington, Nearshore Restoration Assessment

    Science.gov (United States)

    Breems, Joel; Wyllie-Echeverria, Sandy; Grossman, Eric E.; Elliott, Joel

    2009-01-01

    The San Juan Archipelago, located at the confluence of the Puget Sound, the Straits of Juan de Fuca in Washington State, and the Straits of Georgia, British Columbia, Canada, provides essential nearshore habitat for diverse salmonid, forage fish, and bird populations. With 408 miles of coastline, the San Juan Islands provide a significant portion of the available nearshore habitat for the greater Puget Sound and are an essential part of the regional efforts to restore Puget Sound (Puget Sound Shared Strategy 2005). The nearshore areas of the San Juan Islands provide a critical link between the terrestrial and marine environments. For this reason the focus on restoration and conservation of nearshore habitat in the San Juan Islands is of paramount importance. Wood-waste was a common by-product of historical lumber-milling operations. To date, relatively little attention has been given to the impact of historical lumber-milling operations in the San Juan Archipelago. Thatcher Bay, on Blakely Island, located near the east edge of the archipelago, is presented here as a case study on the restoration potential for a wood-waste contaminated nearshore area. Case study components include (1) a brief discussion of the history of milling operations. (2) an estimate of the location and amount of the current distribution of wood-waste at the site, (3) a preliminary examination of the impacts of wood-waste on benthic flora and fauna at the site, and (4) the presentation of several restoration alternatives for the site. The history of milling activity in Thatcher Bay began in 1879 with the construction of a mill in the southeastern part of the bay. Milling activity continued for more than 60 years, until the mill closed in 1942. Currently, the primary evidence of the historical milling operations is the presence of approximately 5,000 yd3 of wood-waste contaminated sediments. The distribution and thickness of residual wood-waste at the site was determined by using sediment

  7. Results of the Washington Passive Solar Design/Build Competition

    Energy Technology Data Exchange (ETDEWEB)

    Nylen, N.

    1981-01-01

    In an effort to encourage the design, construction, and marketing of moderately priced passive solar homes in Washington state, the Western Solar Utilization Network (Western SUN) recently sponsored the Washington Passive Solar Design/Build Competition. The competition drew an overwhelming response from designers and builders throughout Washington. Thermal performance of the designs was evaluated by a technical review committee, and final selections were made by the Competition Jury in accordance with the following criteria: perceived market acceptance, thermal performance, cost effectiveness, simplicity of design and operation, and completeness of the passive concept. Design contract awards totaling $50,000 were made available to winners in four categories, including single and multi-family, new and remodeled residences. In order to receive the award in its entirety, winning design/build teams are required to construct their design by April, 1983. As a result of the competition, a great deal was learned about the attitudes and knowledge of professionals and the general public regarding the use of solar energy in Washington state. Among the points that will be highlighted in this paper are the following: (1) a design/build competition is an effective vehicle for promoting solar energy among professionals in the housing community as well as the general public; (2) passive solar techniques can contribute significantly to the heating and cooling needs of residential housing throughout the state of Washington; (3) there is a great deal of interest and talent among the designers and builders of solar residences in Washington; and (4) follow-up activities, including the promotion of winning designs, the systematic collection of performance data, and identification of the major obstacles confronting designers and builders of solar homes, are critical to the success of the program in achieving both its short-term and long-term goals.

  8. History of Cardiothoracic Surgery at Washington University in Saint Louis.

    Science.gov (United States)

    Moon, Marc R

    2016-01-01

    The Division of Cardiothoracic Surgery at Washington University evolved a century ago to address what many considered to be the last surgical frontier, diseases of the chest. In addition, as one of the first training programs in thoracic surgery, Washington University has been responsible for educating more thoracic surgeons than nearly any other program in the world. Beginning with Evarts A. Graham and continuing through to Ralph J. Damiano Jr., the leaders of the division have had a profound impact on the field of cardiothoracic surgery. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. Papel del colágeno VI en la homeostasis del músculo esquelético y tejido adiposo: implicaciones en la patofisiología de las distrofias musculares

    OpenAIRE

    Rodríguez García, Mª Ángeles

    2016-01-01

    [spa] El colágeno VI es una proteína de la matriz extracelular cuyos defectos conducen a una variedad de trastornos musculares en humanos caracterizados por una debilidad muscular entre otros múltiples síntomas. Tanto el colágeno VI, como su fragmento soluble carboxilo terminal llamado endotrofina se encuentran altamente expresados en los dos principales tejidos periféricos que regulan la glucemia, el músculo esquelético y tejido adiposo. Los niveles de ARNm de colágeno VI en el tejido adipos...

  10. Consumo alimentar de adolescentes matriculados em um colégio particular de Teresina, Piauí, Brasil

    Directory of Open Access Journals (Sweden)

    CARVALHO Cecilia Maria Resende Gonçalves de

    2001-01-01

    Full Text Available Com o objetivo de estudar o consumo alimentar de adolescentes matriculados em um colégio particular de Teresina, realizou-se estudo transversal através de amostragem sistemática. A freqüência de consumo de alimentos expressa em dias por semana foi obtida mediante questionário administrado individualmente ao aluno. O estado nutricional foi avaliado pelo Índice de Massa Corporal, considerando com sobrepeso valores > ou = P85. Os resultados indicam prevalência elevada de risco para o sobrepeso (19,8% nos 334 adolescentes. Os meninos consumiram com maior freqüência alimentos energéticos (milho, manteiga, pizza, lasanha, sucos industrializados, refrigerantes e construtores (frango, ovos, leguminosas em relação às meninas (p < 0,05. Os adolescentes de 10 a 13 anos consumiram com maior freqüência milho, mel, açúcar, rapadura enquanto aqueles com idade entre 17 e 19 anos consumiram mais arroz, bolos, biscoitos, rosca, balas, goma de mascar, refrigerantes, frutas verde-amarelo.

  11. Effect of extracts of plants with insecticidal activity on the control of Microtheca ochroloma Stal (Col: Chrysomelidae in the laboratory

    Directory of Open Access Journals (Sweden)

    Cíntia Grendene Lima

    2008-03-01

    Full Text Available Extracts of plants with insecticidal activity were tested on the control of Microtheca ochroloma (Col.: Chrysomelidae, an important insect-pest of Brassicaceae, in the larval and adult phases. Two 3-day-old larvae, kept under laboratory conditions (25ºC temperature, 70% relative humidity and 14 hours of photophase, were placed in a glass tube with a leaf of Chinese cabbage (Brassica chinensis previously treated with aqueous extracts (10% p/v of chinaberry leaf (Melia azedarach, chinaberry branch, and tobacco powder (Nicotiana tabacum. The same procedure was repeated in two assays with adult insects. In the first assay, all the previously-mentioned extracts were used, in addition to DalNeem (commercial product of Azadirachta indica. In the second, the insects were exposed to extracts of tabasco pepper fruits (Capsicum frutescens, Surinam cherry (Eugenia unifl ora, jambolan (Syzygium cuminii and eucalyptus leaves (Eucalyptus sp.. All the tests consisted of 10 insects per treatment, with five repetitions in the first test using adult insects and six repetitions in the others. Observations were made daily up to the fifth day, aiming to evaluate the mortality of the insects. All the tested extracts resulted in an effective control of the larvae of M. ochroloma. In relation to the adult insects, only the extracts of tobacco powder and DalNeem showed effective control.

  12. Alport Syndrome: De Novo Mutation in the COL4A5 Gene Converting Glycine 1205 to Valine

    Directory of Open Access Journals (Sweden)

    Pilar Antón-Martín

    2012-01-01

    Full Text Available Background Alport syndrome is a primary basement membrane disorder arising from mutations in genes encoding the type IV collagen protein family. It is a genetically heterogeneous disease with different mutations and forms of inheritance that presents with renal affection, hearing loss and eye defects. Several new mutations related to X-linked forms have been previously determined. Methods We report the case of a 12 years old male and his family diagnosed with Alport syndrome after genetic analysis was performed. Result Anew mutation determining a nucleotide change C.3614G > T (p. Gly1205Val in hemizygosis in the COL4A5 gene was found. This molecular defect has not been previously described. Conclusion Molecular biology has helped us to comprehend the mechanisms of pathophysiology in Alport syndrome. Genetic analysis provides the only conclusive diagnosis of the disorder at the moment. Our contribution with a new mutation further supports the need of more sophisticated molecular methods to increase the mutation detection rates with lower costs and less time.

  13. Escolas traçadas com régua e compasso na Colômbia e no Brasil

    Directory of Open Access Journals (Sweden)

    Carlos Jilmar Diaz Soler

    Full Text Available Com este artigo, que é parte de uma pesquisa mais ampla, pretendemos refletir sobre o processo de escolarização da infância, em duas revistas destinadas à formação de professores para o magistério. Ambas produzidas por grupos articulados a políticas educacionais e divulgadas para as redes públicas de ensino de São Paulo (Brasil e outra para Bogotá (Colômbia, de 1932 a 1939. Fizemos duas perguntas às revistas: O que podem nos dizer sobre as finalidades políticas da formação de professores para o magistério? E sobre as relações entre os métodos científicos da psicologia e da pedagogia? No final, apresentamos nossas suspeitas de cumplicidade entre projetos pedagógicos e projetos políticos autoritários, atuando no conjunto das forças cognitivas, psicológicas, culturais e institucionais.

  14. Demarcação e Divulgação dos Limites entre o Brasil e a Colômbia

    OpenAIRE

    Novaes, André Reyes; Palacios, David Ramírez

    2015-01-01

    Os documentos aqui reproduzidos expressam um momento importante no processo de demarcação dos limites entre o Brasil e a Colômbia. O primeiro é a “Acta da vigésima primeira e última conferência da Comissão Mixta Brasileira Colombiana Demarcadora de Limites”, realizada em 5 de janeiro de 1937 na cidade de Manaus. O segundo é a “Carta geográfica da fronteira do Alto Solimões entre Brasil, Perú e Colômbia”, publicada em 1940 com base nos trabalhos da citada comissão, e disponível na Biblioteca D...

  15. Tríplice fronteira Brasil, Peru e Colômbia e as implicações com o narcotráfico

    OpenAIRE

    Dias Balieiro, Luiz Felipe de Vasconcelos; Nascimento, Izaura Rodrigues

    2015-01-01

    Na região do Alto Solimões, extremo oeste do Amazonas, localiza-se a chamada Tríplice Fronteira Amazônica, formada pelas cidades de Tabatinga/Brasil, Santa Rosa/Peru e Letícia/Colômbia. Esta zona fronteiriça é identificada como uma das portas de entrada dos entorpecentes oriundos das zonas produtoras do Peru e Colômbia no território brasileiro. Este artigo objetiva promover a análise das características do narcotráfico na tríplice fronteira amazônica, com uma breve exposição do seu processo d...

  16. Preparación, caracterización y aplicación como plataformas bioactivas de materiales compuestos formados por politiofeno y colágeno

    OpenAIRE

    Illera Bautista, Cristina

    2014-01-01

    El objetivo del trabajo es preparar mezclas compuestas por una proteína, en este caso colágeno tipo I, y un polímero conductor, poli[2,2’-(3-metil-acetato)tiofeno] (P3TMA), un derivado soluble de politiofeno. Para ello se ensayan diferentes metodologías para la preparación de las películas, tales como drop-casting y spin-coating. La proporción de proteína en las mezclas de colágeno/P3TMA es baja, variando entre el 5% y el 0,5% dependiendo de la técnica de preparación empleada, debido a la...

  17. Genetic Variations of the COL4A1 Gene and Intracerebral Hemorrhage Risk: A Case-Control Study in a Chinese Han Population.

    Science.gov (United States)

    Lin, Sen; Xia, Chao; He, Sha; Yang, Jie; Li, Hao; Zheng, Jun; Liu, Ming; You, Chao

    2018-04-01

    To investigate the association between single nucleotide polymorphisms or haplotypes of the COL4A1 gene and the risk of intracerebral hemorrhage (ICH). We conducted a case-control study that included 181 patients from the Chinese Han population with hypertensive ICH and 197 hypertension patients without ICH. Genomic DNA was extracted by DNA extraction kit, and the 6 single nucleotide polymorphism genotypes of the COL4A1 gene were detected with a MassARRAY Analyzer. Unphased 3.1.4 and SPSS 19.0 were used to analyze the association between alleles, genotypes, and haplotypes of the COL4A1 gene and the risk of ICH. Compared with the control group, patients in the ICH group were significantly younger. There were no differences in gender, diabetes, hyperlipidemia, current smoking, and alcohol consumption between the 2 groups. Our association analysis showed that the rs3742207 A, rs11069830 A, and rs679505 A alleles were association factors of the risks of ICH; rs11069830 AA, rs544012 AC, and rs679505 AA genotypes were association factors of the risk of ICH; AA haplotype (rs3742207-rs11069830) was an association factor of the risk of ICH. After adjusting age and gender by multivariate logistic regression, the rs544012 AC and rs679505 AA genotypes were independently associated with the risk of ICH. Our study showed that the rs544012 AC and rs679505 AA genotypes were independently associated with the risk of ICH in the Chinese Han population and that the AA haplotype (rs3742207-rs11069830) in the COL4A1 gene may be related to the risk of ICH in the Chinese Han population; these conclusions need further confirmation in future studies with larger samples. Copyright © 2018. Published by Elsevier Inc.

  18. Diseño de un relleno sanitario manual para el recinto "Cristóbal Colón" - provincia de Esmeraldas

    OpenAIRE

    Ruiz Ortega, Jennifer Carolina; Unapanta Albán, Victoria Carolina

    2015-01-01

    This project, contains the design of a manual sanitary landfill for the recinto Cristóbal Colón, for which it was necessary to know the current situation, with regard to biotic, physical and socio-economic environment. First, is selected representative sample of the population, distributed among households and commercial establishments, in order to collect their waste for eight days and characterized by the quartering method. Once established the optimal way for the design of the landfi...

  19. Cisto de colédoco: experiência de cinco anos com o tratamento cirúrgico no hospital municipal JESUS-RJ

    Directory of Open Access Journals (Sweden)

    Lisieux Eyer de Jesus

    Full Text Available OBJETIVO: Relatar a experiência no diagnóstico e tratamento do cisto de colédoco no Hospital Municipal Jesus em cinco anos (1996-2001 e a experiência brasileira publicada para o tratamento do cisto de colédoco em crianças. MÉTODO: Avaliação prospectiva dos pacientes operados com o diagnóstico de cisto de colédoco em cinco anos no Hospital Municipal Jesus. RESULTADOS: Houve predomínio de casos diagnosticados pela presença de dor abdominal recorrente, icterícia e/ou massa abdominal palpável. Apenas dois pacientes apresentavam a tríade completa. Todos os pacientes foram submetidos à ultra-sonografia e houve dúvida no diagnóstico ultra-sonográfico em apenas dois. Todos eram cistos do tipo 1 de Todani tratados através de ressecção completa e anastomose biliodigestiva em Y Roux. Houve complicações pós-operatórias imediatas em três pacientes: um caso de hemorragia digestiva alta e dois casos de fístula biliar, um deles relacionado a problemas técnicos na anastomose que necessitou de reoperação. O seguimento variou de quatro meses a cinco anos, sem detecção de complicação tardia em nenhum caso. CONCLUSÕES: O tratamento do cisto de colédoco através da ressecção completa na criança é seguro, relacionado a poucas complicações e capaz de resolver por completo a sintomatologia pré-operatória dos pacientes.

  20. Inter-Accions. Una experiència transdisciplinària en un context pedagògic col·lectiu

    Directory of Open Access Journals (Sweden)

    Marta Carrasco-Bonet

    2015-07-01

    Full Text Available Inter-Accions és un projecte pedagògic col·lectiu interuniversitari que treballa des de l'autogestió i la transdisciplinarietat art-arquitectura. És un projecte d’estudiants i per als estudiants d’aquests dos camps que es proposa davant la necessitat d’obrir espais autònoms d’aprenentatge dins la mateixa institució. L’objectiu principal de la proposta és construir un espai d’experimentació on sigui possible treballar sobre la gestió col·lectiva de l’aprenentatge més enllà dels continguts i els resultats. Des d’una perspectiva crítica i en un context acadèmic es proposa aquesta experiència per tal que arquitectes i artistes comparteixin rols i que, a partir del treball col·lectiu i col·laboratiu basat en la idea de codi obert, arribin a establir noves metodologies útils, pràctiques i crítiques aplicables al desenvolupament del seu treball sobre la ciutat. Els projectes que s’han desenvolupat en el marc dels diferents tallers prenen la ciutat com a espai de reflexió i d’intervenció, i permeten arribar a la conclusió que ha estat més determinant l’interès en els mètodes i processos d’aproximació i l’interès en els processos de creació i d’aprenentatge que la voluntat d’aconseguir resultats finalistes. Aquest projecte s’està desenvolupant avui en el marc d’una Assignatura d’Extensió Universitària anomenada “Cartografia Artística” a la Universitat de Barcelona.

  1. Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome.

    Science.gov (United States)

    Chiarelli, Nicola; Carini, Giulia; Zoppi, Nicoletta; Ritelli, Marco; Colombi, Marina

    2018-01-01

    Vascular Ehlers-Danlos syndrome (vEDS) is a dominantly inherited connective tissue disorder caused by mutations in the COL3A1 gene that encodes type III collagen (COLLIII), which is the major expressed collagen in blood vessels and hollow organs. The majority of disease-causing variants in COL3A1 are glycine substitutions and in-frame splice mutations in the triple helix domain that through a dominant negative effect are associated with the severe clinical spectrum potentially lethal of vEDS, characterized by fragility of soft connective tissues with arterial and organ ruptures. To shed lights into molecular mechanisms underlying vEDS, we performed gene expression profiling in cultured skin fibroblasts from three patients with different structural COL3A1 mutations. Transcriptome analysis revealed significant changes in the expression levels of several genes involved in maintenance of cell redox and endoplasmic reticulum (ER) homeostasis, COLLs folding and extracellular matrix (ECM) organization, formation of the proteasome complex, and cell cycle regulation. Protein analyses showed that aberrant COLLIII expression is associated with the disassembly of many structural ECM constituents, such as fibrillins, EMILINs, and elastin, as well as with the reduction of the proteoglycans perlecan, decorin, and versican, all playing an important role in the vascular system. Furthermore, the altered distribution of the ER marker protein disulfide isomerase PDI and the strong reduction of the COLLs-modifying enzyme FKBP22 are consistent with the disturbance of ER-related homeostasis and COLLs biosynthesis and post-translational modifications, indicated by microarray analysis. Our findings add new insights into the pathophysiology of this severe vascular disorder, since they provide a picture of the gene expression changes in vEDS skin fibroblasts and highlight that dominant negative mutations in COL3A1 also affect post-translational modifications and deposition into the ECM of

  2. Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome.

    Directory of Open Access Journals (Sweden)

    Nicola Chiarelli

    Full Text Available Vascular Ehlers-Danlos syndrome (vEDS is a dominantly inherited connective tissue disorder caused by mutations in the COL3A1 gene that encodes type III collagen (COLLIII, which is the major expressed collagen in blood vessels and hollow organs. The majority of disease-causing variants in COL3A1 are glycine substitutions and in-frame splice mutations in the triple helix domain that through a dominant negative effect are associated with the severe clinical spectrum potentially lethal of vEDS, characterized by fragility of soft connective tissues with arterial and organ ruptures. To shed lights into molecular mechanisms underlying vEDS, we performed gene expression profiling in cultured skin fibroblasts from three patients with different structural COL3A1 mutations. Transcriptome analysis revealed significant changes in the expression levels of several genes involved in maintenance of cell redox and endoplasmic reticulum (ER homeostasis, COLLs folding and extracellular matrix (ECM organization, formation of the proteasome complex, and cell cycle regulation. Protein analyses showed that aberrant COLLIII expression is associated with the disassembly of many structural ECM constituents, such as fibrillins, EMILINs, and elastin, as well as with the reduction of the proteoglycans perlecan, decorin, and versican, all playing an important role in the vascular system. Furthermore, the altered distribution of the ER marker protein disulfide isomerase PDI and the strong reduction of the COLLs-modifying enzyme FKBP22 are consistent with the disturbance of ER-related homeostasis and COLLs biosynthesis and post-translational modifications, indicated by microarray analysis. Our findings add new insights into the pathophysiology of this severe vascular disorder, since they provide a picture of the gene expression changes in vEDS skin fibroblasts and highlight that dominant negative mutations in COL3A1 also affect post-translational modifications and deposition

  3. Polymorphisms within the COL5A1 gene and regulators of the extracellular matrix modify the risk of Achilles tendon pathology in a British case-control study.

    Science.gov (United States)

    Brown, Karryn L; Seale, Kirsten B; El Khoury, Louis Y; Posthumus, Michael; Ribbans, William J; Raleigh, Stuart M; Collins, Malcolm; September, Alison V

    2017-08-01

    Several genetic loci have been associated with risk of Achilles tendon pathology (ATP) within South African and Australian populations. The aim of this study was, therefore, to evaluate eight previously implicated genetic variants in an independent British population. A total of 130 asymptomatic controls (CON) and 112 participants clinically diagnosed with ATP comprising 87 individuals with chronic Achilles tendinopathy (TEN) and 25 with Achilles tendon ruptures (RUP) were included. All participants were genotyped for variants within the COL5A1, MIR608, IL-1β, IL-6 and CASP8 genes. Primary findings implicated COL5A1 and CASP8. Three inferred allele combinations constructed from COL5A1 rs12722, rs3196378 and rs71746744 were identified as risk modifiers. The T-C-D combination was associated with increased risk of ATP (P = 0.023) and RUP (P RUP (P = 0.011) and the C-C-D combination was associated with decreased risk of ATP (P = 0.011) and RUP (P = 0.004). The CASP8 rs3834129 DD genotype was associated with decreased risk of TEN (P = 0.020, odds ratio: 0.45, 95% confidence interval: 0.22-0.90) and the CASP8 I-G (rs3834129-rs1045485) inferred allele combination was associated with increased risk of TEN (P = 0.031). This study further highlights the importance of polymorphisms within COL5A1 and CASP8 in the aetiology of ATP.

  4. Circular RNA profiling reveals that circCOL3A1-859267 regulate type I collagen expression in photoaged human dermal fibroblasts

    International Nuclear Information System (INIS)

    Peng, Yating; Song, Xiaojing; Zheng, Yue; Wang, Xinyi; Lai, Wei

    2017-01-01

    Production of type I collagen declines is a main characteristic during photoaging, but the mechanism is still not fully understood. Circular RNAs (circRNAs) are a class of newly identified non-coding RNAs with regulatory potency by sequestering miRNAs like a sponge. It's more stable than linear RNAs, and would be a useful tool for regulation of gene expression. However, the role of circRNAs in collagen expression during photoaging is still unclear. Here we performed deep sequencing of RNA generated from UVA irradiated and no irradiated human dermal fibroblasts (HDFs) and identified 29 significantly differentially expressed circRNAs (fold change ≥ 1.5, P < 0.05), 12 circRNAs were up-regulated and 17 circRNAs were down-regulated.3 most differentially expressed circRNAs were verified by qRT-PCR and the down-regulated circCOL3A1-859267 exhibited the most significantly altered in photoaged HDFs. Overexpression of circCOL3A1-859267 inhibited UVA-induced decrease of type I collagen expression and silencing of it reduced type I collagen intensity. Via a bioinformatic method, 44 miRNAs were predicted to binding with circCOL3A1-859267, 5 of them have been confirmed or predicted to interact with type I collagen. This study show that circCOL3A1-859267 regulate type I collagen expression in photoaged HDFs, suggesting it may be a novel target for interfering photoaging.

  5. Genetic associations of body composition, flexibility and injury risk with ACE, ACTN3 and COL5A1 polymorphisms in Korean ballerinas

    Science.gov (United States)

    Kim, Jun Ho; Jung, Eun Sun; Kim, Chul-Hyun; Youn, Hyeon; Kim, Hwa Rye

    2014-01-01

    [Purpose] The purpose of this study was to exam the association of body composition, flexibility, and injury risk to genetic polymorphisms including ACE ID, ACTN3 RX, and COL5A1 polymorphisms in ballet dancers in Korea. [Methods] For the purpose of this study, elite ballerinas (n = 97) and normal female adults (n = 203) aged 18 to 39 were recruited and these participants were tested for body weight, height, body fat, fat free mass, flexibility, injury risks on the joints and gene polymorphisms (ACE, ACTN3, COL5A1 polymorphism). [Results] As results, the ACE DD genotype in ballerinas was associated with higher body fat and percentage of body fat than the ACE II and ID genotypes (p sports injuries, the ankle injury of the XX-genotyped ballerinas was in significantly more prevalence than the RR and XX-genotyped ballerinas (p < 0.05). According to the odd ratio analysis, XX-genotyped ballerinas have the injury risk on the ankle about 4.7 (95% CI: 1.6~13.4, p < 0.05) times more than the RR and RX-genotyped ballerinas. Meanwhile, the COL5A1 polymorphism in ballerinas has no association with any factors including flexibility and injury risks. [Conclusion] In conclusion, ACE polymorphism and ACTN3 polymorphism were associated with ballerinas' performance capacity; COL5A1 was not associated with any factors of performance of Ballerinas. The results suggested that the ACE DD genotype is associated with high body fat, the ACTN3 XX genotype is associated with low fat-free mass, low flexibility, and higher risk of ankle-joint injury. PMID:25566457

  6. O recrutamento militar na América Portuguesa: o esforço conjunto para a defesa da Colônia do Sacramento (1735-1737

    Directory of Open Access Journals (Sweden)

    Paulo César Possamai

    2004-12-01

    Full Text Available In their effort to defend the Colônia de Sacramento, besieged by the Spanish from October 1735 to September 1737, the Portuguese Crown ordered the mobilization of all human and material resources available in the State of Brazil. This article examines the compulsory recruitment that occurred on this occasion and discusses the living conditions of those men who were taken by force from their homes to be sent to the River Platte area, from where few would return.

  7. Breve nota sobre o II Colóquio Brasil-Japão (Tóquio, 29 de fevereiro - 7 de março de 1968

    Directory of Open Access Journals (Sweden)

    Maria Regina da Cunha Rodrigues

    1968-03-01

    Full Text Available Êsse Colóquio, continuação do I realizado em São Paulo de 25 a 27 de julho de 1966, cujos Anais foram objeto de recente publicação (*, ao lado do saldo positivo que conclaves dêsse tipo alcançam, apresentou algumas falhas que, com a devida vênia, mencionaremos a seguir.

  8. A inserção acadêmica e esportiva da primeira turma feminina no Colégio Militar do Rio de Janeiro

    Directory of Open Access Journals (Sweden)

    Liliana Adiers Lohmann

    2006-09-01

    Full Text Available http://dx.doi.org/10.1590/S0104-026X2006000300005 Os objetivos deste trabalho são contextualizar o ambiente em que se deu a inserção da primeira turma feminina no Colégio Militar do Rio de Janeiro, em 1989, bem como identificar e analisar as representações femininas e masculinas sobre a inserção acadêmica e esportiva dessa primeira turma. A metodologia, qualitativa e etnográfica, fundamenta-se na teoria das representações sociais.1 Os dados provêm da observação participante e de seis entrevistas individuais não-diretivas de “elite”2: três ex-alunas e três homens. Analisam-se também reportagens de jornais e revistas, de 1989 a 1996. Constata-se que cuidado e diferença são constantes no discurso dos dois grupos de informantes. Conclui-se que o incentivo ao esporte, proposto pelo comandante do Colégio e liderado pela professora de Educação Física coautora deste trabalho, influenciou e consolidou o espaço para a participação e integração feminina no Colégio e para o seu êxito acadêmico e esportivo.

  9. A familial case of achondrogenesis type II caused by a dominant COL2A1 mutation and "patchy" expression in the mosaic father.

    Science.gov (United States)

    Forzano, F; Lituania, M; Viassolo, A; Superti-Furga, V; Wildhardt, G; Zabel, B; Faravelli, F

    2007-12-01

    Achondrogenesis type II (ACG2) is the most severe disorder that can be produced by dominant mutations in COL2A1. We report on four pregnancies of an apparently healthy, nonconsanguineous young couple. The father had scoliosis as a child, and has slight body disproportion with short trunk. The first child was born at 32 weeks and died neonatally. In the second pregnancy, short limbs and fetal hygroma were noted on ultrasound at 17 weeks' gestation. Similar findings were observed in the third fetus. Clinical, radiological, and histological evaluation of the fetuses after termination of the pregnancies showed findings consistent with ACG2. Molecular analysis of genomic DNA extracted from amniotic cells of the second and third fetuses revealed heterozygosity for a 10370G > T missense mutation (G346V) in the COL2A1 gene. This mutation was also found in the father, as a mosaic. The couple had a fourth pregnancy, and at 11 weeks fetal hydrops with a septated cystic hygroma were obvious. DNA from CVS demonstrated the same COL2A1 mutation. (c) 2007 Wiley-Liss, Inc.

  10. Hello Mr. President! Rollenspiele zwischen Hollywood und Washington

    NARCIS (Netherlands)

    Mehring, F.

    2016-01-01

    Hello Mr. President Rollenspiele zwischen Hollywood und Washington Am 8. November 2016 entscheidet sich, wer zum 58. Präsidenten der USA gewählt wird: Die ehemalige Außenministerin Hillary Clinton oder der republikanische Kandidat Donald Trump. In der heißen Phase des Wahlkampfs fällt besonders

  11. Experience with Honeycrisp apple storage management in Washington

    Science.gov (United States)

    High demand and premium pricing have led to rapid increases in Honeycrisp plantings and fruit volume in Washington State, USA since introduction of the cultivar in 1999. Most fruit is packed and sold by January because of strong demand coupled with difficulties associated with extended storage. Howe...

  12. The State of Washington's Children, Summer 2002. [Tenth Annual Report].

    Science.gov (United States)

    Connell, Frederick A.; Brandon, Richard; Hill, Sheri L.; Carter, S. Louise; Garrison, Michelle M.; DeWys, Shelley; Mandell, Dorothy J.

    This Kids Count report is the tenth to examine annually statewide trends in the well-being of Washington's children and focuses on child poverty and the needs of the working poor. The statistical portrait is based on indicators of child well-being in five areas: (1) family and community, including teen birth rate, teen pregnancy rate, births to…

  13. Booker T. Washington's Audacious Vocationalist Philosophy

    Science.gov (United States)

    Lewis, Theodore

    2014-01-01

    Booker T. Washington was born a slave in the American South, rising remarkably in the period after slavery to become a leader of his race. His advocacy of appeasement with the Southern white establishment incurred the ire of his black peers, given the withdrawal of the franchise from ex-slaves in southern states after a brief period of positive…

  14. Digital Learning Compass: Distance Education State Almanac 2017. Washington

    Science.gov (United States)

    Seaman, Julia E.; Seaman, Jeff

    2017-01-01

    This brief report uses data collected under the U.S. Department of Education's National Center for Educational Statistics (NCES) Integrated Postsecondary Education Data System (IPEDS) Fall Enrollment survey to highlight distance education data in the state of Washington. The sample for this analysis is comprised of all active, degree-granting…

  15. 78 FR 46258 - Drawbridge Operation Regulation Lake Washington, Seattle, WA

    Science.gov (United States)

    2013-07-31

    ... that governs the Evergreen Point Floating Bridge (State Route 520 across Lake Washington) at Seattle... Department of Transportation has requested that the draw span of the Evergreen Point Floating Bridge (State... this time, which would divert road traffic onto the Evergreen Point Floating Bridge. The closure of the...

  16. Managing the Development of the Public Telecommunications Center, Spokane, Washington.

    Science.gov (United States)

    Schaar, Walter

    When the city council of Spokane (Washington) decided in 1971 to establish a cable franchise, it created a citizens' committee to set cable specifications. Representing Spokane School District 81 and KSPS-TV (a public television station licensed to the public schools of Spokane), the author of this document served on the committee that set five…

  17. The State of Washington's Children, Fall 2001. [Ninth Annual Report].

    Science.gov (United States)

    Washington Univ., Seattle. School of Public Health and Community Medicine.

    This Kids Count report is the ninth to examine annually statewide trends in the well-being of Washington's children. The statistical portrait is based on indicators of child well-being in five areas: (1) family and community, including teen birth rate, teen pregnancy rate, divorces involving children, and births to unmarried mothers; (2) economic…

  18. The State of Washington's Children. [Seventh Annual Report].

    Science.gov (United States)

    Marvinney, Sandy, Ed.

    This KIDS COUNT seventh annual report examines statewide trends in the well-being of Washington's children. The statistical portrait is based 24 key indicators of well-being: (1) teen birth rate; (2) teen pregnancy rate; (3) births to unmarried mothers; (4) divorces involving children; (5) family foster caseload; (6) average real wages; (7) per…

  19. Productivity of nonindustrial private forests in western Washington: alternative futures.

    Science.gov (United States)

    Ralph J. Alig; Darius M. Adams

    1995-01-01

    Nonindustrial private timberlands in western Washington have high productive potential and contribute harvest amounts somewhat more than proportional to their area. Of all private ownerships they are influenced the most by land use shifts and are affected in important ways by forest practice regulations. About 1 million acres of nonindustrial private timberland contain...

  20. Composition at Washington State University: Building a Multimodal Bricolage

    Science.gov (United States)

    Ericsson, Patricia; Hunter, Leeann Downing; Macklin, Tialitha Michelle; Edwards, Elizabeth Sue

    2016-01-01

    Multimodal pedagogy is increasingly accepted among composition scholars. However, putting such pedagogy into practice presents significant challenges. In this profile of Washington State University's first-year composition program, we suggest a multi-vocal and multi-theoretical approach to addressing the challenges of multimodal pedagogy. Patricia…

  1. Timber resource statistics for Washington, January 1, 1973.

    Science.gov (United States)

    Patricia M. Bassett; Grover A. Choate

    1974-01-01

    Timber resource statistics to January 1, 1973, for the State of Washington show total land area, commercial timberland area, and growing stock and sawtimber inventory volumes by county and owner group. Growth and removals are shown by Forest Survey inventory unit for 1972. Each National Forest is updated to January 1, 1973.

  2. Large-scale silviculture experiments of western Oregon and Washington.

    Science.gov (United States)

    Nathan J. Poage; Paul D. Anderson

    2007-01-01

    We review 12 large-scale silviculture experiments (LSSEs) in western Washington and Oregon with which the Pacific Northwest Research Station of the USDA Forest Service is substantially involved. We compiled and arrayed information about the LSSEs as a series of matrices in a relational database, which is included on the compact disc published with this report and...

  3. Alternative Fuels Data Center: Washington Transportation Data for

    Science.gov (United States)

    stations in Washington with alternative fuels Fuel Public Private Biodiesel (B20 and above) 8 33 Compressed Partnerships Spark Biodiesel Success for Essential Baking Company Partnerships Spark Biodiesel Success for Videos on YouTube Video thumbnail for Seattle Bakery Delivers With Biodiesel Trucks Seattle Bakery

  4. A 2015 comparison of operational performance : Washington state ferries to ferry operators worldwide.

    Science.gov (United States)

    2015-03-01

    This report provides an update to the 2010 report A Comparison of Operational Performance: : Washington State Ferries to Ferry Operators Worldwide, observing changes in Washington State : Ferries, 23 other ferry systems, and the ferry industry ...

  5. 77 FR 51563 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

    Science.gov (United States)

    2012-08-24

    ... Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA AGENCY: National... Anthropology, has completed an inventory of human remains and associated funerary objects, in consultation with... of Washington, Department of Anthropology. Disposition of the human remains and associated funerary...

  6. Marijuana, other drugs, and alcohol use by drivers in Washington State.

    Science.gov (United States)

    2016-07-01

    In Washington State legal sales of marijuana began July 8, 2014. A voluntary, anonymous roadside study was conducted to assess the prevalence of drivers testing positive for alcohol and other drugs, including marijuana, on Washingtons roads. Data ...

  7. Marijuana, other drugs, and alcohol use by drivers in Washington state : appendices.

    Science.gov (United States)

    2016-07-01

    In Washington State legal sales of marijuana began July 8, 2014. A voluntary, anonymous roadside study was conducted to assess the prevalence of drivers testing positive for alcohol and other drugs, including marijuana, on Washingtons roads. Data ...

  8. 78 FR 13887 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

    Science.gov (United States)

    2013-03-01

    ... Washington State Parks and Recreation Commission at the address below by April 1, 2013. ADDRESSES: Alicia... contact Alicia Woods, Washington State Parks and Recreation Commission, PO Box 42650, Olympia, WA 98504...

  9. 77 FR 48535 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

    Science.gov (United States)

    2012-08-14

    ...: Alicia Woods, Washington State Parks and Recreation Commission, P.O. Box 42650, Olympia, WA 98504-2650... it satisfies the criteria in 43 CFR 10.11(c)(1) should contact Alicia Woods, Washington State Parks...

  10. 77 FR 61782 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

    Science.gov (United States)

    2012-10-11

    ... Washington State Parks and Recreation Commission at the address below by November 13, 2012. ADDRESSES: Alicia... affiliated with the human remains should contact Alicia Woods, Washington State Parks and Recreation...

  11. Death with dignity in Washington patients with amyotrophic lateral sclerosis.

    Science.gov (United States)

    Wang, Leo H; Elliott, Michael A; Jung Henson, Lily; Gerena-Maldonado, Elba; Strom, Susan; Downing, Sharon; Vetrovs, Jennifer; Kayihan, Paige; Paul, Piper; Kennedy, Kate; Benditt, Joshua O; Weiss, Michael D

    2016-11-15

    To describe the amyotrophic lateral sclerosis (ALS) patients who sought medication under the Washington State Death with Dignity (DWD) Act since its inception in 2009. Chart review at 3 tertiary medical centers in the Seattle/Puget Sound region and comparison to publicly available data of ALS and all-cause DWD cohorts from Washington and Oregon. In Washington State, 39 patients with ALS requested DWD from the University of Washington, Virginia Mason, and Swedish Medical Centers beginning in 2009. The median age at death was 65 years (range 46-86). Seventy-seven percent of the patients used the prescriptions. All of the patients who used the medications passed away without complications. The major reasons for patients to request DWD as reported by participating physicians were loss of autonomy and dignity and decrease in enjoyable activities. Inadequate pain control, financial cost, and loss of bodily control were less commonly indicated. These findings were similar to those of the 92 patients who sought DWD in Oregon. In Washington and Oregon, the percentage of patients with ALS seeking DWD is higher compared to the cancer DWD cohort. Furthermore, compared to the all-cause DWD cohort, patients with ALS are more likely to be non-Hispanic white, married, educated, enrolled in hospice, and to have died at home. Although a small number, ALS represents the disease with the highest proportion of patients seeking to participate in DWD. Patients with ALS who choose DWD are well-educated and have access to palliative or life-prolonging care. The use of the medications appears to be able to achieve the patients' goals without complications. © 2016 American Academy of Neurology.

  12. SIRT1 deacetylates RFX5 and antagonizes repression of collagen type I (COL1A2) transcription in smooth muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Xia, Jun [Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University (China); Department of Respiratory Medicine, Jiangsu Provincial Hospital of Chinese Traditional Medicine (China); Wu, Xiaoyan; Yang, Yuyu; Zhao, Yuhao [Atherosclerosis Research Center, Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Pathophysiology, Nanjing Medical University (China); Fang, Mingming [Jiangsu Jiankang Vocational Institute (China); Xie, Weiping, E-mail: wpxienjmu@gmail.com [Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University (China); Wang, Hong, E-mail: hwangnjmu@gmail.com [Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University (China); Xu, Yong [Atherosclerosis Research Center, Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Pathophysiology, Nanjing Medical University (China)

    2012-11-16

    Highlights: Black-Right-Pointing-Pointer SIRT1 interacts with and deacetylates RFX5. Black-Right-Pointing-Pointer SIRT1 activation attenuates whereas SIRT1 inhibition enhances collagen repression by RFX5 in vascular smooth muscle cells. Black-Right-Pointing-Pointer SIRT1 promotes cytoplasmic localization and proteasomal degradation of RFX5 and cripples promoter recruitment of RFX5. Black-Right-Pointing-Pointer IFN-{gamma} represses SIRT1 expression in vascular smooth muscle cells. Black-Right-Pointing-Pointer SIRT1 agonist alleviates collagen repression by IFN-{gamma} in vascular smooth muscle cells. -- Abstract: Decreased expression of collagen by vascular smooth muscle cells (SMCs) within the atherosclerotic plaque contributes to the thinning of the fibrous cap and poses a great threat to plaque rupture. Elucidation of the mechanism underlying repressed collagen type I (COL1A2) gene would potentially provide novel solutions that can prevent rupture-induced complications. We have previously shown that regulatory factor for X-box (RFX5) binds to the COL1A2 transcription start site and represses its transcription. Here we report that SIRT1, an NAD-dependent, class III deacetylase, forms a complex with RFX5. Over-expression of SIRT1 or NAMPT, which synthesizes NAD+ to activate SIRT1, or treatment with the SIRT1 agonist resveratrol decreases RFX5 acetylation and disrupts repression of the COL1A2 promoter activity by RFX5. On the contrary, knockdown of SIRT1 or treatment with SIRT1 inhibitors induces RFX5 acetylation and enhances the repression of collagen transcription. SIRT1 antagonizes RFX5 activity by promoting its nuclear expulsion and proteasomal degradation hence dampening its binding to the COL1A2 promoter. The pro-inflammatory cytokine IFN-{gamma} represses COL1A2 transcription by down-regulating SIRT1 expression in SMCs. Therefore, our data have identified as novel pathway whereby SIRT1 maintains collagen synthesis in SMCs by modulating RFX5 activity.

  13. Efeitos do conflito armado sobre a vida e a saúde na Colômbia

    Directory of Open Access Journals (Sweden)

    Saúl Franco

    Full Text Available O presente trabalho trata das conseqüências do conflito armado interno (CAI que a Colômbia tem vivido durante as últimas quatro décadas. Começa identificando o contexto e o momento atual do conflito. A seguir, são descritas algumas das formas, expressões e conseqüências do conflito armado interno, com maior impacto sobre a vida, a qualidade de vida, a saúde e as doenças da população e sobre os serviços de saúde no país. Especial ênfase é dada às altas taxas de mortalidade por homicídio, ao fenômeno do deslocamento forçado pela violência, aos seqüestros e às minas antipessoais. Entre os grupos populacionais mais afetados, destacam-se homens jovens, mulheres, crianças e as minorias étnicas indígenas e afrodescendentes. Esta análise também se refere ao sério problema das contínuas infrações da Lei Internacional Humanitária e aos ataques contra a Missão Médica, assim como às conseqüências negativas do conflito sobre a prestação de serviços de saúde. Para concluir, são feitos alguns comentários gerais sobre a situação descrita e dadas algumas sugestões para o estudo do problema e possíveis ações partindo do setor saúde.

  14. Basic Education for Adults: Pathways to College and Careers for Washington's Emerging Workforce. Washington's Community and Technical Colleges

    Science.gov (United States)

    Washington State Board for Community and Technical Colleges, 2015

    2015-01-01

    This brief describes the Basic Education for Adults (BEdA) programs that bridge the gap between school and work, thereby creating pathways to college and careers for Washington's emerging workforce. BEdA programs teach foundational skills--reading, writing, math, technology and English language--so adults can move through college and into…

  15. 78 FR 59964 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2013-09-30

    ....S.C. 3003, of the completion of an inventory of human remains under the control of the Burke Museum....R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of... Washington (Burke Museum), and Central Washington University have completed an inventory of human remains, in...

  16. 78 FR 64006 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2013-10-25

    ... inventory of human remains under the control of the Burke Museum. The human remains were removed from Island....R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of... Memorial Washington State Museum, University of Washington (Burke Museum), has completed an inventory of...

  17. 78 FR 59955 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2013-09-30

    ... completion of an inventory of human remains under the control of the Burke Museum, Seattle, WA. The human....R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of... Memorial Washington State Museum, University of Washington (Burke Museum), has completed an inventory of...

  18. 78 FR 53235 - 50th Anniversary of the March on Washington for Jobs and Freedom

    Science.gov (United States)

    2013-08-28

    ... Anniversary of the March on Washington for Jobs and Freedom By the President of the United States of America A... waters of the reflecting pool, to the proud base of the Washington Monument. They were men and women..., and justice for all. The March on Washington capped off a summer of discontent, a time when the...

  19. 76 FR 58033 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2011-09-19

    ... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum (Burke...

  20. 77 FR 46117 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2012-08-02

    ... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-10823; 2200-1100-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington...

  1. 78 FR 11675 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2013-02-19

    ... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-12080;2200-1100-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington...

  2. 75 FR 36671 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2010-06-28

    ... DEPARTMENT OF THE INTERIOR National Park Service Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA; Correction AGENCY: National Park... human remains and associated funerary objects in the possession of the Thomas Burke Memorial Washington...

  3. 76 FR 58034 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2011-09-19

    ... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum (Burke...

  4. 76 FR 58031 - Notice of Intent To Repatriate Cultural Items: Thomas Burke Memorial Washington State Museum...

    Science.gov (United States)

    2011-09-19

    ... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Intent To Repatriate Cultural Items: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum...

  5. 76 FR 58039 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2011-09-19

    ... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum (Burke...

  6. 77 FR 50157 - Notice of Public Meeting, Eastern Washington Resource Advisory Council Meeting

    Science.gov (United States)

    2012-08-20

    ...; HAG 12-0260] Notice of Public Meeting, Eastern Washington Resource Advisory Council Meeting AGENCY.... Department of the Interior, Bureau of Land Management (BLM) Eastern Washington Resource Advisory Council... Bureau of Land Management's Eastern Washington and San Juan Resource Management Plan and the U.S. Forest...

  7. 77 FR 23495 - Notice of Public Meeting, Eastern Washington Resource Advisory Council Meeting

    Science.gov (United States)

    2012-04-19

    ...; HAG 12-0164] Notice of Public Meeting, Eastern Washington Resource Advisory Council Meeting AGENCY... 1972, the U.S. Department of the Interior, Bureau of Land Management (BLM) Eastern Washington Resource... of Land Management's Eastern Washington and San Juan Resource Management Plan, and the U.S. Forest...

  8. Racismo y derechos humanos en Colombia: avatares de las poblaciones afrocolombianas; Racismo e direitos humanos na Colômbia: avatares das populações afro- colombianas; Racism and human rights in Colombia: avatars of the Afro-Colombian peoples

    Directory of Open Access Journals (Sweden)

    Adriana Espinosa Bonilla

    2015-07-01

    Full Text Available Resumen: Este artículo pretende plantear una reflexión acerca de la forma en que se ha configurado el racismo frente a las poblaciones negras en Colombia y cómo las luchas en el campo político del movimiento social afrocolombiano, han esgrimido el discurso de los derechos humanos como estrategia de resistencia. Dicho análisis, describe las interacciones de los representantes del gobierno con los activistas de las organizaciones negras, en el escenario de las audiencias ante la Comisión Interamericana de Derechos Humanos en Washington, donde aparecen los discursos relacionados de racismo y derechos humanos, en la contienda entre representantes del estado y comunidades negras.   Palabras clave: Racismo, Derechos Humanos, ciudadanía, Identidad, Movimiento Social, Organizaciones Negras.       Resumo: Este artigo pretende expor uma reflexão sobre a forma como se configurou o racismo frente as populações negras na Colômbia e como as lutas no campo político do movimento social afro-colombiano brandiu o discurso dos direitos humanos como estratégia de resistência. Essa analise descreve as interações dos representantes do governo com os ativistas das organizações negras no cenário das audiências na Corte Interamericana de Direitos Humanos, em Washington, onde aparecem discursos relacionados com o racismo e os direitos humanos, nas disputas entre representantes do Estado e das comunidades negras.   Palavras-chave: Racismo, DIreitos Humanos, cidadania, identidade, Movimento social, Organizações Negras.       Abstract: This article aims at presenting a reflection on how racism against black populations in Colombia has been configured and how the fights in the political     field of the Afro-Colombian social movement have employed the discourse of human rights as a strategy of resistance. Such analysis describes the interactions between the government representatives and the activists of black organizations on the stage of the

  9. Porous polymer monolithic col

    Directory of Open Access Journals (Sweden)

    Lydia Terborg

    2015-05-01

    Full Text Available A new approach has been developed for the preparation of mixed-mode stationary phases to separate proteins. The pore surface of monolithic poly(glycidyl methacrylate-co-ethylene dimethacrylate capillary columns was functionalized with thiols and coated with gold nanoparticles. The final mixed mode surface chemistry was formed by attaching, in a single step, alkanethiols, mercaptoalkanoic acids, and their mixtures on the free surface of attached gold nanoparticles. Use of these mixtures allowed fine tuning of the hydrophobic/hydrophilic balance. The amount of attached gold nanoparticles according to thermal gravimetric analysis was 44.8 wt.%. This value together with results of frontal elution enabled calculation of surface coverage with the alkanethiol and mercaptoalkanoic acid ligands. Interestingly, alkanethiols coverage in a range of 4.46–4.51 molecules/nm2 significantly exceeded that of mercaptoalkanoic acids with 2.39–2.45 molecules/nm2. The mixed mode character of these monolithic stationary phases was for the first time demonstrated in the separations of proteins that could be achieved in the same column using gradient elution conditions typical of reverse phase (using gradient of acetonitrile in water and ion exchange chromatographic modes (applying gradient of salt in water, respectively.

  10. Opportunities for addressing laminated root rot caused by Phellinus sulphuracens in Washington's forests: A Report from the Washington State Academy of Sciences in cooperation with the Washington State Department of Natural Resources

    Science.gov (United States)

    R. James Cook; Robert L. Edmonds; Ned B. Klopfenstein; Willis Littke; Geral McDonald; Daniel Omdahl; Karen Ripley; Charles G. Shaw; Rona Sturrock; Paul Zambino

    2013-01-01

    This report from the Washington State Academy of Sciences (WSAS) is in response to a request from the Washington State Department of Natural Resources (DNR) to "identify approaches and opportunities ripe for research on understanding and managing root diseases of Douglas-fir." Similar to the process used by the National Research Council, the WSAS upon...

  11. Reaching Higher. A Parent's Guide to the Washington Assessment of Learning. Revised = Para llegar mas arriba. Una guia para padres sobre la evaluacion del aprendizaje de los estudiantes del estado de Washington (Washington Assessment of Student Learning). Revisado.

    Science.gov (United States)

    Washington Office of the State Superintendent of Public Instruction, Olympia.

    This guide in English and Spanish is designed to answer questions parents may have about the Washington Assessment of Student Learning (WASL), including how it will help improve their children's education, how it is scored, and how to use the information it provides. In Washington, clear educational goals for subject content, thinking skills, and…

  12. Routine environmental audit of the Hanford Site, Richland, Washington

    Energy Technology Data Exchange (ETDEWEB)

    1994-05-01

    This report documents the results of the routine environmental audit of the Hanford Site (Hanford), Richland, Washington. During this audit, the activities conducted by the audit team included reviews of internal documents an reports from previous audits and assessments; interviews with US Department of Energy (DOE), State of Washington regulatory, and contractor personnel; and inspections and observations of selected facilities and operations. The onsite portion of the audit was conducted May 2--13, 1994, by the DOE Office of Environmental Audit (EH-24), located within the Office of Environment, Safety and Health (EH). The audit evaluated the status of programs to ensure compliance with Federal, State, and local environmental laws and regulations; compliance with DOE orders, guidance, and directives; and conformance with accepted industry practices and standards of performance. The audit also evaluated the status and adequacy of the management systems developed to address environmental requirements.

  13. El Consenso de Washington: aciertos, yerros y omisiones

    Directory of Open Access Journals (Sweden)

    Juan Carlos Moreno-Brid

    2004-12-01

    Full Text Available This paper analyses the economic and social development of Latin America after nearly two decades of macroeconomic policies and reforms in line with the “Washington Consensus”. It shows that these policies did lower inflation and induced an export boom, but failed to boost domestic investment and to remove the balance of payments binding constraint on the region’s long–term path of economic expansion. Four alternative explanations of such poor performance of the Washington Consensus are compared. It is argued, in particular, that, contrary to mainstream opinion, in Latin America there is no clear association between the depth of macroeconomic reforms and economic growth performance.

  14. Routine environmental audit of the Hanford Site, Richland, Washington

    International Nuclear Information System (INIS)

    1994-05-01

    This report documents the results of the routine environmental audit of the Hanford Site (Hanford), Richland, Washington. During this audit, the activities conducted by the audit team included reviews of internal documents an reports from previous audits and assessments; interviews with US Department of Energy (DOE), State of Washington regulatory, and contractor personnel; and inspections and observations of selected facilities and operations. The onsite portion of the audit was conducted May 2--13, 1994, by the DOE Office of Environmental Audit (EH-24), located within the Office of Environment, Safety and Health (EH). The audit evaluated the status of programs to ensure compliance with Federal, State, and local environmental laws and regulations; compliance with DOE orders, guidance, and directives; and conformance with accepted industry practices and standards of performance. The audit also evaluated the status and adequacy of the management systems developed to address environmental requirements

  15. Status of birds at the Hanford Site in southeastern Washington

    International Nuclear Information System (INIS)

    Landeen, D.S.; Johnson, A.R.; Mitchell, R.M.

    1992-06-01

    The US Department of Energy has entered into agreements with the Washington State Department of Ecology, the US Environmental Protection Agency, and Hanford Site contractors to focus work activities on cleanup and stabilization of radioactive and hazardous waste sites located at the Hanford Site in southeastern Washington. Ecological characterization is an essential part of the remediation process, and the identification of biotic components such as bird species that could be impacted by cleanup activities is an important part of the initial environmental characterizations. Site characterization work has resulted in this list of 238 birds that have been observed at the Hanford Site. This list is presented with a status rating for abundance and seasonal occurrence

  16. Groundwater levels for selected wells in Upper Kittitas County, Washington

    Science.gov (United States)

    Fasser, E.T.; Julich, R.J.

    2011-01-01

    Groundwater levels for selected wells in Upper Kittitas County, Washington, are presented on an interactive, web-based map to document the spatial distribution of groundwater levels in the study area measured during spring 2011. Groundwater-level data and well information were collected by the U.S. Geological Survey using standard techniques and are stored in the U.S. Geological Survey National Water Information System, Groundwater Site-Inventory database.

  17. Memorial—John A. Washington II, M.D.▿

    OpenAIRE

    2010-01-01

    John A. Washington II, M.D., former Head of Clinical Microbiology at the Mayo Clinic from 1972 to 1986 and Chairman of the Department of Microbiology at the Cleveland Clinic from 1986 to 1992, died on 5 September 2010 at the age of 74. John was an internationally recognized, widely respected leader in the disciplines of clinical microbiology and infectious diseases, authoring more than 450 scientific articles, books, and book chapters and training scores of pathology residents and clinical mi...

  18. Volatile substance misuse deaths in Washington State, 2003-2012.

    Science.gov (United States)

    Ossiander, Eric M

    2015-01-01

    Volatile substance misuse (VSM - also known as huffing or sniffing) causes some deaths, but because there are no specific cause-of-death codes for VSM, these deaths are rarely tabulated. Count and describe VSM deaths occurring in Washington State during 2003-2012. We used the textual cause-of-death information on death certificates to count VSM-associated deaths that occurred in Washington State during 2003-2012. We extracted records that contained words suggesting either a method of inhalation or a substance commonly used for VSM, and reviewed those records to identify deaths on which the inhalation of a volatile substance was mentioned. We conducted a descriptive analysis of those deaths. Fifty-six deaths involving VSM occurred in Washington State during 2003-2012. VSM deaths occurred primarily among adults age 20 and over (91%), males (88%), and whites (93%). Twelve different chemicals were associated with deaths, but 1 of them, difluoroethane, was named on 30 death certificates (54%), and its involvement increased during the study period. Gas duster products were named as the source of difluoroethane for 12 deaths; no source was named for the other 18 difluoroethane deaths. Most VSM deaths occurred among white male adults, and gas duster products containing difluoroethane were the primary source of inhalants. Approaches to deter VSM, such as the addition of bitterants to gas dusters, should be explored.

  19. Genetic screening of a pedigree with osteogenesis imperfecta type Ⅰand identification of a novel mutation in COL1A2 pathogenic gene%成骨不全Ⅰ型家系的基因检测和COL1A2基因新突变的致病性鉴定

    Institute of Scientific and Technical Information of China (English)

    李荣; 郭源平; 潘敬新; 郭奕斌

    2015-01-01

    To uncover the molecular pathogenic mechanism of congenital osteogenesis imperfecta (OI) type I, all the 103 exons of the COL1A1 (Collagen, type Ⅰ, alpha 1) and COL1A2 (Collagen, type Ⅰ, alpha 2) genes in a child with OI type Ⅰ were screened using PCR-DNA direct sequencing. The results showed no pathological mutation in COL1A1 gene, but a novel mutation c.946G>T/p.G316C in the exon 19 of COL1A2 gene, which was inherited from her father. This mutation was not found in her mother and other six phenotypically normal relatives. By denaturing high perfor-mance liquid chromatography (DHPLC) screening, the abnormal double-peak was visualized in PCR products of exon 19 of COL1A2 gene in the proband and her father, while the normal single-peak was shown in those of her mother and all the healthy controls. Using allele specific amplification (ASA) screening, a specific band of 391 bp in COL1A2 exon 19 was amplified only in the proband and her father, but not in other samples. The amino acid encoded by the mutation site is evolutionarily highly conserved, and this mutation was a“damaging”or“probably damaging”factor to OI type Ⅰ, based on the predicting results using SIFT and Polyphen-2 softwares. In conclusion, the novel c.946G>T/p.G316C mutation in COL1A2 gene is a pathogenic mutation that could result in OI type Ⅰ. If the couple wants to get pregnant again, it is necessary to screen the mutation site in COL1A2 gene through the prenatal genetic diagnosis in the first trimester or through preimplantation genetic diagnosis (PGD) in the progestation.%为了揭示成骨不全(Osteogenesis imperfecta, OI)Ⅰ型家系的分子遗传学发生机制,文章采用PCR-DNA直接测序法,对患儿 COL1A1和 COL1A2基因共103个外显子(E)进行突变检测。结果显示:患儿 COL1A1基因未发现任何病理性突变,而在 COL1A2基因 E19内发现一新的杂合错义突变(p.G316C),该突变来自其父,而其母正常,其他表型正常的6位

  20. Delineation of Ehlers-Danlos syndrome phenotype due to the c.934C>T, p.(Arg312Cys) mutation in COL1A1: Report on a three-generation family without cardiovascular events, and literature review.

    Science.gov (United States)

    Colombi, Marina; Dordoni, Chiara; Venturini, Marina; Zanca, Arianna; Calzavara-Pinton, Piergiacomo; Ritelli, Marco

    2017-02-01

    Classical Ehlers-Danlos syndrome (cEDS) is a rare connective tissue disorder primarily characterized by hyperextensible skin, defective wound healing, abnormal scars, easy bruising, and generalized joint hypermobility; arterial dissections are rarely observed. Mutations in COL5A1 and COL5A2 encoding type V collagen account for more than 90% of the patients so far characterized. In addition, cEDS phenotype was reported in a small number of patients carrying the c.934C>T mutation in COL1A1 that results in an uncommon substitution of a non-glycine residue in one Gly-Xaa-Yaa repeat of the pro-α1(I)-chain p.(Arg312Cys), which leads to disturbed collagen fibrillogenesis due to delayed removal of the type I procollagen N-propeptide. This specific mutation has been associated with propensity to arterial rupture in early adulthood; indeed, in literature the individuals harboring this mutation are also referred to as "(classic) vascular-like" EDS patients. Herein, we describe a three-generation cEDS family with six adults carrying the p.(Arg312Cys) substitution, which show a variable and prevalent cutaneous involvement without any major vascular event. These data, together with those available in literature, suggest that vascular events are not a diagnostic handle to differentiate patients with the p.(Arg312Cys) COL1A1 mutation from those with COL5A1 and COL5A2 defects, and highlight that during the diagnostic process the presence of at least the p.(Arg312Cys) substitution in COL1A1 should be investigated in cEDS patients without type V collagen mutations. Nevertheless, for these patients, as well as for those affected with cEDS, a periodical vascular surveillance should be carried out together with cardiovascular risk factors monitoring. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  1. The effect of 6-week high intensity interval training on the VEGF/COL-18 ratio and some echocardiographic indices in rats with myocardial infarction

    Directory of Open Access Journals (Sweden)

    Sara Karbalaeifar

    2016-12-01

    Full Text Available Introduction: Myocardial infarction (MI is the irreversible cell death caused by ischemia in parts of myocardium. The molecular process of increased capillary density in response to activity and its appropriate intensity is not clear yet. Therefore, this research aimed to evaluate the effect of 6-week high intensity interval training on the VEGF/COL-18 ratio and echocardiographic indices in rats with MI. Methods: Twelve Wistar male rats of 10 weeks old and mean weight 250-300gr were allocated to two groups of experimental (60 minutes of interval treadmill running for four minutes with the intensity of 85-90 and two minutes of active rest at 50-60 percent of VO2max for four days a week for 6 weeks and control group (without any training. Real-time PCR was used to assess the expression of VEGF and COL-18 genes after inducing MI, and shortening fractional and ejection fraction were investigated as echocardiographic indices. Data were analyzed in SPSS18 using independent t test (α≤ 0.05. Results: The findings showed that there was no significant increase in the VEGF/COL-18 ratio in the HIIT group (1.856 mg/ml as compared with the control group (1.245 mg/ml (p=0.263. A significant increase was observed in the HIIT group for SF (77.461±7.022% and EF (41.625±6.847% as compared with the control group (64.483±3.695% and (31.320±3.460%, respectively (p=0.001. Conclusion: In general, 6 weeks of high intensity interval training can effectively increase angiogenesis factors and improve myocardial function in male Wistar rats after MI.

  2. O berço francês na nossa educação: do projeto do Colégio Pedro II ao Bac-ENEM

    Directory of Open Access Journals (Sweden)

    Cynthia Agra de Brito Neves

    2015-12-01

    Full Text Available Este artigo tem por finalidade refletir sobre as raízes e os vínculos transversais do Brasil com a França a partir da investigação das heranças e influências da pedagogia francesa na formação do nosso ensino secundário, sobretudo em meados do século XIX, quando o Colégio Pedro II, a escola modelo, era referência nacional e importava currículos e programas de estudos dos lycées franceses. Almeida (2000, Doria (1997, Santos (2003, Vechia e Cavazotti et al (2003 e, principalmente, Razzini (2000 foram contribuições preciosas para constatarmos o papel das ideias libertárias e literárias dessa pedagogia na excelência desse Colégio no período do Império à República no Brasil. Criado em 1837, sob tutela do Estado e com o nome do imperador, o Colégio estabelecia um projeto de nação baseado nessas ideias. A instituição – responsável pela formação de nomes como Joaquim Manuel de Macedo, Manuel Bandeira e Pedro Nava – contava com um corpo docente de prestígio que se dedicou, durante décadas, à formação dos filhos da elite brasileira que procurava naquela escola uma educação inspirada no modelo clássico e humanista francês.

  3. Upregulated expression of integrin α1 in mesangial cells and integrin α3 and vimentin in podocytes of Col4a3-null (Alport mice.

    Directory of Open Access Journals (Sweden)

    Brooke M Steenhard

    Full Text Available Alport disease in humans, which usually results in proteinuria and kidney failure, is caused by mutations to the COL4A3, COL4A4, or COL4A5 genes, and absence of collagen α3α4α5(IV networks found in mature kidney glomerular basement membrane (GBM. The Alport mouse harbors a deletion of the Col4a3 gene, which also results in the lack of GBM collagen α3α4α5(IV. This animal model shares many features with human Alport patients, including the retention of collagen α1α2α1(IV in GBMs, effacement of podocyte foot processes, gradual loss of glomerular barrier properties, and progression to renal failure. To learn more about the pathogenesis of Alport disease, we undertook a discovery proteomics approach to identify proteins that were differentially expressed in glomeruli purified from Alport and wild-type mouse kidneys. Pairs of cy3- and cy5-labeled extracts from 5-week old Alport and wild-type glomeruli, respectively, underwent 2-dimensional difference gel electrophoresis. Differentially expressed proteins were digested with trypsin and prepared for mass spectrometry, peptide ion mapping/fingerprinting, and protein identification through database searching. The intermediate filament protein, vimentin, was upregulated ∼2.5 fold in Alport glomeruli compared to wild-type. Upregulation was confirmed by quantitative real time RT-PCR of isolated Alport glomeruli (5.4 fold over wild-type, and quantitative confocal immunofluorescence microscopy localized over-expressed vimentin specifically to Alport podocytes. We next hypothesized that increases in vimentin abundance might affect the basement membrane protein receptors, integrins, and screened Alport and wild-type glomeruli for expression of integrins likely to be the main receptors for GBM type IV collagen and laminin. Quantitative immunofluorescence showed an increase in integrin α1 expression in Alport mesangial cells and an increase in integrin α3 in Alport podocytes. We conclude that

  4. Autógrafos de Cristobal Colón: Ensayo de Análisis Grafológico de su Escritura

    Directory of Open Access Journals (Sweden)

    Boletín Cultural y Bibliográfico Banco de la República

    1968-02-01

    Full Text Available Absolutamente el único mapa o dibujo topográfico que resta de Colón. De este marino, que toda su vida confeccionó mapas, que de modo seguro tuvo que haber dibujado las derrotas de sus viajes, que se sabe que regaló un gran mapa a Isabel, solo queda este pedazo de papel (hoy en el Archivo de la Casa de Alba, en Madrid, "misteriosamente adquirido por la Duquesa de Alba", según Thacher (I, p. 477, quien lo reproduce en (III, p. 8 9 y dice : "Evidentemente dibujado en enero de 1493.

  5. Celularidade do leite e Unidades Formadoras de Colônias nas mastites causadas por Staphylococcus coagulase positiva e coagulase negativa

    Directory of Open Access Journals (Sweden)

    Helio Langoni

    2015-06-01

    Full Text Available Resumo: A mastite é a principal afecção do gado destinado à produção leiteira, que impacta significativamente a cadeia produtiva do leite, com reflexos ainda para a saúde pública. Estudou-se aspectos relacionados à etiologia, celularidade e de contagem bacteriana em 10 propriedades leiteiras, localizadas no Estado de São Paulo. Foram examinadas 1148 vacas em lactação, totalizando 4584 glândulas mamárias. Foram considerados os casos, em que houve isolamento de estafilococos coagulase positiva (SCP e estafilococos coagulase negativa (SCN. Os resultados revelaram microbiota com vários patógenos e diferentes espécies de SCN (128 casos e SCP (45, Staphylococcus aureus(90, Streptococcus agalactiae(70, Streptococcus dysgalactiae (69, Streptococcus uberis(29, Corynebacteriumspp. (230, Klebsiella pneumoniae(28, Klebsiella oxytoca(2, Escherichia coli(15, Enterobactersp. (3. Os resultados de contagem de células somáticas (CCS relacionados aos SCP e SCN não mostraram diferenças entre as propriedades avaliadas, entretanto com diferenças significantes ao se avaliar a CCS entre os dois grupos de estafilococos, como pode ser evidenciado ao comparar SCN Discreto e SCP exuberante (P<0,01, SCP Discreto e SCP exuberante (P<0,001 e SCN moderado e SCP exuberante (P<0,01. A avaliação da CCS relacionada à intensidade da infecção, considerando-se como crescimento discreto o isolamento de até nove colônias, moderado de dez a 29 colônias e exuberante, com 30 ou mais colônias, revelou para ambos os grupos de estafilococos que quanto maior o número de unidades formadoras de colônias (UFC, a CCS é mais elevada, sendo sempre maior nos casos de SCP. Conclui-se que quando há maior número de UFC, há concomitantemente maior CCS/mL de leite, no caso dos SCP e SCN, o que mostra relação direta da intensidade do processo infeccioso com a resposta da celularidade do leite, bem como pela relevância desses na etiologia das mastites e dos aspectos

  6. Desenvolvimento de colônias de abelhas com diferentes alimentos protéicos Development of honeybee colonies under protein diets

    Directory of Open Access Journals (Sweden)

    Fábia de Mello Pereira

    2006-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o desempenho de produtos regionais do Nordeste na alimentação de colônias de abelhas (Apis mellifera, em um período de escassez de floradas. Foram fornecidas dietas às abelhas, contendo 20% de proteína bruta, à base de feno de mandioca (Manihot esculenta e farinha de vagem de algaroba (Prosopis juliflora, feno de mandioca e farelo de babaçu (Orbygnia martiana, farelo de babaçu e Purilac (sucedâneo para bezerros da marca Purina e pólen apícola de Palmae. As colônias foram analisadas quanto ao peso e às áreas de alimento e cria. Não foi observada diferença significativa entre os tratamentos em relação às áreas de cria. Apesar de a pasta com pólen ser a mais consumida, este alimento mostrou conversão alimentar menor do que as demais dietas fornecidas. As colônias que receberam pasta de feno de mandioca com farelo de babaçu tiveram maior peso final. Todos os alimentos mostraram-se eficientes na manutenção das colônias.The objective of this work was to evaluate the efficiency of some regional products of Brazil Northeast to feed Apis mellifera colonies. Diets with 20% of crude protein made of cassava hay (Manihot esculenta and mesquite pod meal (Prosopis juliflora, cassava hay and babassu bran (Orbygnia martiana, babassu bran and Purilac (succedaneous for calfskin from Purina and Palmae pollen were offered to the honeybees. Colonies were evaluated for weight gain, store area and brood area. There was no significant difference among the treatments in relation to the brood areas. Pollen treatment showed the highest intake but also showed the lowest food conversion. Beehives that received diet with cassava hay and babassu flour showed greater final weight gain. All diets were efficient in the maintenance of the colonies.

  7. Presença de cinco rainhas fisogástricas em colônia de abelha sem ferrão (Melipona scutellaris Latreille)

    OpenAIRE

    Carvalho, Carlos Alfredo Lopes de; Souza, Bruno de Almeida; Dias, Carleandro de Souza; Alves, Rogério Marcos de Oliveira; Melo, Alex Fábio de Lima; Soares, Ana Cristina Fermino; Carvalho-Zilse, Gislene Almeida

    2011-01-01

    Polygyny, characterized by the presence of several egg-laying queens, is considered as a temporary colony status. In stingless bees it is rarely observed. This paper reports the first case of natural polygyny in Melipona scutellaris colony, with five egg-laying queens.Poliginia, caracterizada pela presença de mais de uma rainha poedeira, é considerada como uma condição temporária em colônias. Em abelhas sem ferrão isso é raramente observado. Este artigo registra o primeiro caso de poliginia n...

  8. Taxa de Infestação da Varroa destructor em colônias de Apis mellifera L. no Agreste Meridional de Pernambuco

    Directory of Open Access Journals (Sweden)

    Deygnon Cavalcanti Clementino

    2016-08-01

    Full Text Available O presente trabalho teve como objetivo verificar a taxa de infestação da Varroa destructor em colônias de Apis mellifera L. proveniente de apiário no município de Lagoa do Ouro, Agreste Meridional de Pernambuco. A pesquisa foi realizada no mês de setembro de 2015. Participaram do estudo 15 colônias, em que foram coletadas em média 227 abelhas adultas de cada colmeia e acondicionadas em recipientes contendo álcool a 70%. Após as coletas a campo, as amostras foram transportadas para o laboratório de Biologia Animal da Universidade Federal Rural de Pernambuco/Unidade Acadêmica de Garanhuns, para a realização das análises e quantificação dos ácaros. Os resultados demonstraram que 100% das amostras estavam parasitadas com o ácaro, sendo que a média geral da taxa de infestação foi de 6,16%. O grau de infestação pela Varroa destructor observado nas colônias variou de 0,93% a 11,15%. Conclui-se que as colônias analisadas apresentam diferentes níveis de infestação pelo ácaro Varroa destructor.Infestation rate of Varroa destructor in Apis mellifera L. colonies in the South Agreste of PernambucoAbstract: This study aimed to verify the infestation rate of Varroa destructor in Apis mellifera L. colonies in the apiary from Lagoa do Ouro County, South Agreste of Pernambuco. The survey was conducted in September 2015. The study included 15 colonies, which were collected on average 227 adult bees in each hive and placed in vials containing 70% alcohol. After harvesting the field, the samples were transported to the Animal Biology Laboratory at the Federal Rural University of Pernambuco/Academic Unit of Garanhuns, to perform the analysis and quantification of mites. The results showed that 100% of the samples were infested with mites, and the overall average infestation rate was 6.16%. The degree of infestation by Varroa destructor observed in colonies ranged from 0.93% to 11.15%. It is concluded that the analyzed colonies have

  9. Imagens das colônias da empresa de colonização Meyer no Rio Grande do Sul, 1906

    Directory of Open Access Journals (Sweden)

    Rosane Marcia Neumann

    2013-04-01

    Full Text Available Esse artigo, a partir da análise do prospecto fotográfico Ansichten aus Dr. Herrmann Meyers Ackerbaukolonien Neu-Württemberg und Xingu in Rio Grande do Sul (Südbrasilien, editado pelo Instituto Bibliográfico de Leipzig, em 1906, discute o uso da fotografia pela Empresa de Colonização Dr. Herrmann Meyercomo suporte para a divulgação de seu projeto de colonização, e o modo pelo qual representou as suas colônias e os seus colonos.

  10. Análise da expressão do colágeno VI na distrofia muscular congênita Analysis of the expression of collagen VI in congenital muscular dystrophy

    Directory of Open Access Journals (Sweden)

    Regina Toni Loureiro de Freitas

    2005-06-01

    Full Text Available A distrofia muscular congênita (DMC compõe um grupo de miopatias caracterizadas por hipotonia e fraqueza muscular notadas já no primeiro ano de vida. A forma de Ullrich é caracterizada por retrações musculares proximais e hiperextensibilidade distal. Cerca de 40% destes pacientes apresentam mutações em um dos genes que codificam as três sub-unidades do colágeno VI (COL6, acarretando deficiência total ou parcial na marcação da proteína. Analisamos, através de imunofluorescência, a marcação do COL6 em fragmentos musculares de 50 pacientes com DMC, 20 deles com ausência da marcação para merosina. Identificamos 4 casos com deficiência total da marcação do COL6 (8% do total, representando 13% dos casos com marcação normal para merosina. As alterações histológicas musculares dos pacientes com COL6 deficiente eram indistinguíveis das outras formas de DMC, porém mais brandas que as observadas na DMC com deficiência de merosina. Em três dos pacientes com COL6 deficiente observou-se hipotonia e fraqueza muscular, notadas já no período neonatal, atraso do desenvolvimento motor, retrações musculares em joelhos e cotovelos, hiperextensibilidade distal e luxação congênita do quadril (dois pacientes. Um paciente perdeu a capacidade para a marcha, e outro faleceu por problemas respiratórios. A análise da marcação do COL6, assim como da merosina, no tecido muscular de pacientes com DMC pode auxiliar na identificação e caracterização fenotípica dos diversos subtipos de DMC.Congenital muscular dystrophy (CMD composes a group of disorders characterized by hypotonia and muscular weakness noticed in the first year of life. The Ullrich's form is characterized by proximal joint contractures and distal hiperextensibility. About 40% of these patients present mutations in one of the genes that codify the sub-units of the collagen VI protein (COL6, producing total or partial deficiency of the protein expression. We analyzed

  11. Next-generation sequencing and a novel COL3A1 mutation associated with vascular Ehlers-Danlos syndrome with severe intestinal involvement: a case report.

    Science.gov (United States)

    Cortini, Francesca; Marinelli, Barbara; Seia, Manuela; De Giorgio, Barbara; Pesatori, Angela Cecilia; Montano, Nicola; Bassotti, Alessandra

    2016-10-31

    The vascular type of Ehlers-Danlos syndrome is an autosomal dominant connective tissue disorder caused by a mutation in the COL3A1 gene encoding pro-alpha1 chain of type III collagen. The vascular type of Ehlers-Danlos syndrome causes severe fragility of connective tissues with arterial and intestinal ruptures and complications in surgical and radiological treatments. We present a case of a 38-year-old Italian woman who was diagnosed as having the vascular type of Ehlers-Danlos syndrome. Genetic testing, conducted by Target Enrichment approach (Agilent Technologies), identified a new mutation c.1493G>A, p.G498D in exon 21 of COL3A1 gene (heterozygous state). This mutation disrupts the normal glycine-X-Y repetitions of type III procollagen by converting glycine to aspartic acid. We report a new genetic mutation associated with the vascular type of Ehlers-Danlos syndrome. We also describe clinical and genetic findings that are important to understand the genotype/phenotype correlation in patients with the vascular type of Ehlers-Danlos syndrome.

  12. DESARROLLO DE COMPETENCIAS EN LOS ESTUDIANTES DEL LABORATORIO DE MATERIALES DEL PROGRAMA DE INGENIERÍA MECÁNICA EN EL NÚCLEO LUZ-COL.

    Directory of Open Access Journals (Sweden)

    Raysa Balza

    2012-11-01

    Full Text Available Esta investigación tiene como objetivo analizar las estrategias que contribuyen al desarrollo de competencias en los estudiantes del laboratorio de Materiales en el Programa de Ingeniería Mecánica Núcleo LUZ-COL. La investigación se catalogó como descriptiva, con un diseño de campo, no experimental, transeccional (Hernández y col., 2003. La población estuvo integrada or seis (6 docentes y 365 estudiantes. Seleccionando una muestra de 191 estudiantes, los otros elementos de la población fueron considerados en su totalidad. Se utilizaron como instrumentos una guía de observación y la entrevista estructurada. Evidenciando que el laboratorio presenta debilidades tanto en los aspectos infraestructurales como académicos; las estrategias utilizadas contribuyen medianamente a la formación de competencias en los estudiantes a través de un aprendizaje cooperativo. Asimismo, los equipos utilizados no cumplen con los aspectos tecnológicos y didácticos para el desarrollo de las prácticas.

  13. Enhanced Wnt signaling improves bone mass and strength, but not brittleness, in the Col1a1(+/mov13) mouse model of type I Osteogenesis Imperfecta.

    Science.gov (United States)

    Jacobsen, Christina M; Schwartz, Marissa A; Roberts, Heather J; Lim, Kyung-Eun; Spevak, Lyudmila; Boskey, Adele L; Zurakowski, David; Robling, Alexander G; Warman, Matthew L

    2016-09-01

    Osteogenesis Imperfecta (OI) comprises a group of genetic skeletal fragility disorders. The mildest form of OI, Osteogenesis Imperfecta type I, is frequently caused by haploinsufficiency mutations in COL1A1, the gene encoding the α1(I) chain of type 1 collagen. Children with OI type I have a 95-fold higher fracture rate compared to unaffected children. Therapies for OI type I in the pediatric population are limited to anti-catabolic agents. In adults with osteoporosis, anabolic therapies that enhance Wnt signaling in bone improve bone mass, and ongoing clinical trials are determining if these therapies also reduce fracture risk. We performed a proof-of-principle experiment in mice to determine whether enhancing Wnt signaling in bone could benefit children with OI type I. We crossed a mouse model of OI type I (Col1a1(+/Mov13)) with a high bone mass (HBM) mouse (Lrp5(+/p.A214V)) that has increased bone strength from enhanced Wnt signaling. Offspring that inherited the OI and HBM alleles had higher bone mass and strength than mice that inherited the OI allele alone. However, OI+HBM and OI mice still had bones with lower ductility compared to wild-type mice. We conclude that enhancing Wnt signaling does not make OI bone normal, but does improve bone properties that could reduce fracture risk. Therefore, agents that enhance Wnt signaling are likely to benefit children and adults with OI type 1. Copyright © 2016 Elsevier Inc. All rights reserved.

  14. Confirmation of the pathogenicity of a mutation p.G337C in the COL1A2 gene associated with osteogenesis imperfecta

    Science.gov (United States)

    Jia, Mingrui; Shi, Ranran; Zhao, Xuli; Fu, Zhijian; Bai, Zhijing; Sun, Tao; Zhao, Xuejun; Wang, Wenbo; Xu, Chao; Yan, Fang

    2017-01-01

    Abstract Mutation analysis as the gold standard is particularly important in diagnosis of osteogenesis imperfecta (OI) and it may be preventable upon early diagnosis. In this study, we aimed to analyze the clinical and genetic materials of an OI pedigree as well as to confirm the deleterious property of the mutation. A pedigree with OI was identified. All family members received careful clinical examinations and blood was drawn for genetic analyses. Genes implicated in OI were screened for mutation. The function and structure of the mutant protein were predicted using bioinformatics analysis. The proband, a 9-month fetus, showed abnormal sonographic images. Disproportionately short and triangular face with blue sclera was noticed at birth. She can barely walk and suffered multiple fractures till 2-year old. Her mother appeared small stature, frequent fractures, blue sclera, and deformity of extremities. A heterozygous missense mutation c.1009G>T (p.G337C) in the COL1A2 gene was identified in her mother and her. Bioinformatics analysis showed p.G337 was well-conserved among multiple species and the mutation probably changed the structure and damaged the function of collagen. We suggest that the mutation p.G337C in the COL1A2 gene is pathogenic for OI by affecting the protein structure and the function of collagen. PMID:28953610

  15. Effect in field of the native strain COL6 of Rhizobium leguminosarum bv. viciae on common bean cv. Percal in Ecuador

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    Klever Iván Granda-Mora

    2017-07-01

    Full Text Available Inoculation with selected Rhizobium strains of common bean (Phaseolus vulgaris L. contributes to the agricultural yield of this legume, improves soil fertility and reduces the use of nitrogen fertilizers in sustainable agricultural systems. The research aimed to determine the effect in field of the native strain Rhizobium leguminosarum bv. viciae COL6 on growth, N fixation and agricultural yield in common bean cv. Percal. A field trial was carried out to evaluate the parameters of nodulation, biomass, nitrogen fixation, yield components and agricultural yield in common bean. In addition, soil analyzes were performed before and after experimentation. Prior to planting an inoculant was formulated with the strain COL6. The seeds of cv. Percal were mixed in 250 g inoculant per 10 kg of seed and it was sown manually in the soil. The results of growth, N fixation (total N % and agricultural yield demonstrated the positive effect of inoculation with the bacterial inoculant comparable with chemical fertilizers. The increase in agricultural yield with bacterial inoculant application and chemical fertilization with respect to control was 62 and 64 %, respectively. The results of the soil analysis were variable with respect to the initial physical-chemical characteristics. The promising results of bacterial inoculant use and the effective stimulation in common bean, open the doors for its potential use in Ecuadorian soils and with that the desired potential yield can be reached.

  16. Biblioteca do Colégio Estadual Manoel Vilaverde: um espaço além das quatro paredes

    Directory of Open Access Journals (Sweden)

    Maria Aparecida Rodrigues de Souza

    2005-07-01

    Full Text Available Relato de experiência da prestação de serviços e atividades de leitura desenvolvidas na Biblioteca Domingos Garcia Filho do Colégio Estadual Manoel Vilaverde, em Inhumas – Goiás. Este relato faz um apanhado das estratégias para estímulo à leitura praticadas pela BDGF desde a sua reativação em 2001; ano em que o colégio foi contemplado com mais de 1300 livros pelo Programa de Bibliotecas das Escolas Estaduais do Estado de Goiás. Ilustramos através de fotos a participação efetiva dos alunos nos projetos desenvolvidos pela biblioteca. Durante o período de 2001 a 2004 foram desenvolvidos dois grandes projetos: Minutos de Leitura e o Projeto Pró-melhoria dos recursos pedagógicos do CEM. Projetos estes que envolvem diretamente a biblioteca, responsável pela mediação do usuário com a leitura e a pesquisa.

  17. Cristóbal Colón como puente trasatlántico en el imaginario de Eugenio María de Hostos

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    Rodríguez de Laguna, Asela

    2015-12-01

    Full Text Available Since Eugenio María de Hostos’ publication in 1863 of La peregrinación de Bayoán until 1892, Columbus occupies a central space in his writings. The transformation of Columbus from a Romantic to a Positivist hero serves to exhibit the ideological, historical and political tensions of a colonial Puerto Rican subject questioning his historical and political legacy. It also triggers the examination not only of the figure of Columbus, Spain and the conquest, but of the Nineteenth century complex reality of the American, Caribbean and Latin American nation states.Desde la publicación de La peregrinación de Bayoán (1863 hasta el 1892 la escritura de Eugenio María de Hostos evidencia un especial interés por la figura de Cristóbal Colón. La centralidad del Descubridor, así como su transformación de genio romántico a positivista en el discurso hostosiano pone de relieve las tensiones históricas, políticas e ideológicas de un sujeto puertorriqueño colonial con respecto a su legado histórico-político. Sirve para cuestionar no solo la figura del mismo Colón, sino la conquista, y la situación de los pueblos de las dos Américas en el siglo diecinueve.

  18. Immobilization of cross linked Col-I–OPN bone matrix protein on aminolysed PCL surfaces enhances initial biocompatibility of human adipogenic mesenchymal stem cells (hADMSC)

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Young-Hee; Jyoti, Md. Anirban; Song, Ho-Yeon, E-mail: songmic@sch.ac.kr

    2014-06-01

    In bone tissue engineering surface modification is considered as one of the important ways of fabricating successful biocompatible material. Addition of biologically active functionality on the surfaces has been tried for improving the overall biocompatibility of the system. In this study poly-ε-caprolactone film surfaces have been modified through aminolysis and immobilization process. Collagen type I (COL-I) and osteopontin (OPN), which play an important role in osteogenesis, was immobilized onto PCL films followed by aminolysis treatment using 1,6-hexanediamine. Characterization of animolysed and immobilized surfaces were done by a number techniques using scanning electron microscopy (SEM), FT-IR, XPS, ninhydrin staining, SDS-PAGE and confocal microscopy and compared between the modified and un-modified surfaces. Results of the successive experiments showed that aminolysis treatment was homogeneously achieved which helped to entrap or immobilize Col-I–OPN proteins on surfaces of PCL film. In vitro studies with human adipogenic mesenchymal stem cells (hADMSC) also confirmed the attachment and proliferation of cells was better in modified PCL surfaces than the unmodified surfaces. SEM, confocal microscopy and MTT assay showed a significant increase in cell spreading, attachment and proliferations on the biofunctionalized surfaces compared to the unmodified PCL surfaces at all-time points indicating the success of surface biofunctionalization.

  19. siRNA-mediated Allele-specific Silencing of a COL6A3 Mutation in a Cellular Model of Dominant Ullrich Muscular Dystrophy

    Directory of Open Access Journals (Sweden)

    Véronique Bolduc

    2014-01-01

    Full Text Available Congenital muscular dystrophy type Ullrich (UCMD is a severe disorder of early childhood onset for which currently there is no effective treatment. UCMD commonly is caused by dominant-negative mutations in the genes coding for collagen type VI, a major microfibrillar component of the extracellular matrix surrounding the muscle fibers. To explore RNA interference (RNAi as a potential therapy for UCMD, we designed a series of small interfering RNA (siRNA oligos that specifically target the most common mutations resulting in skipping of exon 16 in the COL6A3 gene and tested them in UCMD-derived dermal fibroblasts. Transcript analysis by semiquantitative and quantitative reverse transcriptase PCR showed that two of these siRNAs were the most allele-specific, i.e., they efficiently knocked down the expression from the mutant allele, without affecting the normal allele. In HEK293T cells, these siRNAs selectively suppressed protein expression from a reporter construct carrying the mutation, with no or minimal suppression of the wild-type (WT construct, suggesting that collagen VI protein levels are as also reduced in an allele-specific manner. Furthermore, we found that treating UCMD fibroblasts with these siRNAs considerably improved the quantity and quality of the collagen VI matrix, as assessed by confocal microscopy. Our current study establishes RNAi as a promising molecular approach for treating dominant COL6-related dystrophies.

  20. Organics Verification Study for Sinclair and Dyes Inlets, Washington

    Energy Technology Data Exchange (ETDEWEB)

    Kohn, Nancy P.; Brandenberger, Jill M.; Niewolny, Laurie A.; Johnston, Robert K.

    2006-09-28

    Sinclair and Dyes Inlets near Bremerton, Washington, are on the State of Washington 1998 303(d) list of impaired waters because of fecal coliform contamination in marine water, metals in sediment and fish tissue, and organics in sediment and fish tissue. Because significant cleanup and source control activities have been conducted in the inlets since the data supporting the 1998 303(d) listings were collected, two verification studies were performed to address the 303(d) segments that were listed for metal and organic contaminants in marine sediment. The Metals Verification Study (MVS) was conducted in 2003; the final report, Metals Verification Study for Sinclair and Dyes Inlets, Washington, was published in March 2004 (Kohn et al. 2004). This report describes the Organics Verification Study that was conducted in 2005. The study approach was similar to the MVS in that many surface sediment samples were screened for the major classes of organic contaminants, and then the screening results and other available data were used to select a subset of samples for quantitative chemical analysis. Because the MVS was designed to obtain representative data on concentrations of contaminants in surface sediment throughout Sinclair Inlet, Dyes Inlet, Port Orchard Passage, and Rich Passage, aliquots of the 160 MVS sediment samples were used in the analysis for the Organics Verification Study. However, unlike metals screening methods, organics screening methods are not specific to individual organic compounds, and are not available for some target organics. Therefore, only the quantitative analytical results were used in the organics verification evaluation. The results of the Organics Verification Study showed that sediment quality outside of Sinclair Inlet is unlikely to be impaired because of organic contaminants. Similar to the results for metals, in Sinclair Inlet, the distribution of residual organic contaminants is generally limited to nearshore areas already within the

  1. Prevalence of corporal punishment among students in Washington State schools.

    Science.gov (United States)

    Grossman, D C; Rauh, M J; Rivara, F P

    1995-05-01

    To determine the prevalence of corporal punishment in Washington State and the factors associated with its use in Washington elementary and secondary schools. Cross-sectional mail survey performed during the summer of 1992. All elementary and secondary schools in the state of Washington. One thousand eighteen schools (47%) responded to the survey, of which 80% were publicly funded and 63% were located in urban areas. The study sample closely resembled the profile of all schools in the state. Almost 11% of participating schools permitted corporal punishment at the time of the survey and 3.2% reported its actual use during the 1991-1992 school year, resulting in an estimated prevalence of 7.2 incidents per 1000 students per year. Sixteen percent of corporal punishment actions occurred in schools not permitting its use. Ninety percent of public schools relied on district policy regarding corporal punishment. School characteristics associated with the use of corporal punishment included rural location (crude odds ratio, 2.2; 95% confidence interval, 1.5 to 3.4), enrollment of less than 500 students (crude odds ratio, 1.7; 95% confidence interval, 1.1 to 2.7), and kindergarten to eighth-grade or kindergarten to 12th-grade enrollment (crude odds ratio, 2.5; 95% confidence interval, 1.6 to 3.9). The lack of a statewide ban on school corporal punishment at the time of this survey was associated with the continued use of corporal punishment against children in districts that continued to permit it. School policies against corporal punishment were associated with much lower prevalence. Continued efforts are needed to enact and enforce laws in the remaining states that have not yet banned corporal punishment.

  2. Landslides and engineering geology of the Seattle, Washington, area

    Science.gov (United States)

    Baum, Rex L.; Godt, Jonathan W.; Highland, Lynn M.

    2008-01-01

    This volume brings together case studies and summary papers describing the application of state-of-the-art engineering geologic methods to landslide hazard analysis for the Seattle, Washington, area. An introductory chapter provides a thorough description of the Quaternary and bedrock geology of Seattle. Nine additional chapters review the history of landslide mapping in Seattle, present case studies of individual landslides, describe the results of spatial assessments of landslide hazard, discuss hydrologic controls on landsliding, and outline an early warning system for rainfall-induced landslides.

  3. Details of microearthquake swarms in the Columbia basin, Washington

    International Nuclear Information System (INIS)

    Malone, S.D.; Rothe, G.H.; Smith, S.W.

    1975-01-01

    Three microearthquake swarms in the Columbia River basin of eastern Washington were studied by means of a small portable seismic network. Earthquakes in this area typically occur in swarms, concentrated both temporally and spatially. One unusual characteristic of the three swarms studied was the shallow focal depths of all events. Most events located had depths less than 1 km; none were deeper than 2 km. Composite focal mechanism solutions indicate that more than one fault surface is active in any one swarm. All events had some thrust component with the axis of maximum compression oriented roughly in a north-south direction. (auth)

  4. Surficial geology map of the Great Heath, Washington County, Maine

    Science.gov (United States)

    Cameron, Cornelia Clermont; Mullen, Michael K.

    1983-01-01

    The major portion of the Great Heath, comprising 2,645 acres in the Cherryfield quadrangle, Washington County, Maine, generally averaging 13 feet in thickness, but with as great an average as 15 feet, contain an estimated 6,953 ,000 short tons air-dried peat. The peat #s chiefly sphagnum moss with some reed-sedge of high quality according to ASTM standards for agricultural and horticultural use. This same volume of peat may be considered for use as fuel because BTO per pound ranges from 8,600 to 10,500 with low sulfur and high hydrogen contents.

  5. Mixed waste management in Washington and the Northwest Compact Region

    International Nuclear Information System (INIS)

    Carlin, E.M.

    1988-01-01

    The state of Washington's concerns about the management of mixed waste have evolved over the past year. One concern that receives increasing attention is the Northwest Compact Region's need to plan for disposal of its own mixed waste. An informal survey of the region's potential mixed waste generators has indicated that mixed waste volumes are low. However, the opening of a disposal facility may result in increased waste volumes. A preliminary proposal for such a facility has been reviewed by the federal and state agencies that dually regulate mixed waste. Initial conclusions reached by the regulators are presented

  6. Seismic reflection survey conducted in Benton and Grant Counties, Washington

    International Nuclear Information System (INIS)

    Durham, T.E.; Beggs, H.G.; Heineck, R.L.

    1979-01-01

    The following report is devoted to a discussion of data acquisition procedures, data processing parameters and interpretation of results for a reflection seismic survey located on the Hanford Site in Benton and Grant Countries, Washington. The Pasco basin was the geologic setting for the survey. The main objectives of the program were to determine the subsurface structural attitudes of the numerous basalt flows known to exist within the basin. The location of areas associated with possible faulting and/or fracturing was also considered of prime concern as these conditions could significantly affect the integrity of the basalt

  7. Hydrologic bibliography of the Columbia River basalts in Washington

    International Nuclear Information System (INIS)

    Tanaka, H.H.; Wildrick, L.

    1978-07-01

    This bibliography is part of the hydrologic data compilation effort of the Columbia Plateau Hydrology Study, Rockwell Hanford Operations' Waste Isolation Program. It includes references on both surface and subsurface hydrology directly or indirectly related to the Washington State portion of the Columbia River basalts. A comprehensive, annotated bibliography of the Pasco Basin (including the Hanford site) hydrology has been prepared for Rockwell Hanford Operations under the Pasco Basin Hydrology Study. In order to avoid unnecessary duplication, no effort was made to include a complete list of bibliographic references on Hanford in this volume

  8. The FLIP fuel experience at Washington State University

    International Nuclear Information System (INIS)

    Lovas, Thomas A.

    1977-01-01

    The Washington State University TRIGA-fueled modified G.E. reactor was refueled with a partial TRIGA-FLIP core in February, 1976. The final core loading consisted of 35 FLIP and 75 Standard TRIGA fuel rods and provided a core excess reactivity of $7.98. The observed performance of the reactor did not deviate significantly from the design predictions and specifications. Pulsing tests revealed a maximum power output of 1850 MW with a fuel temperature of 449 deg. C from a $2.50 pulse. Slight power fluctuations at 1 Megawatt steady-state operation and post-pulse power oscillations were observed. (author)

  9. Investigando a construção de sentidos sobre o ambiente em visitas de crianças a um colégio agrícola

    OpenAIRE

    Arante, Juliana de Souza Neves

    2009-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Educação Científica e Tecnológica, Florianópolis, 2009 É prática comum, no Colégio Agrícola Senador Carlos Gomes de Oliveira (CASCGO), localizado no município de Araquari - SC, as visitas programadas de turmas de outros colégios para que conheçam como funciona uma escola-fazenda. Para que esta atividade ocorra, o colégio conta com seus próprios alunos como guias dessas visitas. O objetivo principa...

  10. Compósito de colágeno com silicato e hidroxiapatita como material para endodontia: preparação e caracterização = Collagen composite with silicate and hydroxyapatite as endodontic material: preparation and characterization

    Directory of Open Access Journals (Sweden)

    E'Gues, Miguel Antônio Menezes

    2008-01-01

    Full Text Available Objetivo: Este trabalho teve por objetivo preparar e caracterizar um novo compósito de colágeno com silicato e hidroxiapatita que possa vir a ser utilizado como material endodôntico em capeamento pulpar direto e em pulpotomia. Metodologia: Os materiais utilizados como matéria-prima foram colágeno do tipo I na forma aniônica, silicato (cimento Portland Branco e hidroxiapatita sintetizada. Estes materiais foram misturados em proporções variadas de modo a obter um material compósito na forma de pasta. Os materiais obtidos foram caracterizados por técnicas de calorimetria exploratória diferencial, termogravimetria, microscopia eletrônica de varredura (MEV, espectrometria de dispersão de energia de Raios-X e espectroscopia na região do infravermelho. Resultados: A presença de colágeno no compósito foi demonstrada por transições térmicas típicas daquelas encontradas para o colágeno do tipo I (~48ºC, independentemente da proporção utilizada, e também por imagens de MEV, onde as fibras colagênicas também puderam ser observadas interligando as partículas de silicato. A composição química de todos compósitos de colágeno foi praticamente idêntica à do silicato utilizado. Conclusão: Os resultados mostraram que a estrutura da matriz de colágeno nos compósitos testados não foi alterada. Esta é uma característica importante para a utilização em endodontia do material compósito proposto, objetivando uma reparação tecidual de forma "mais natural"

  11. Surface hydrologic investigations of the Columbia Plateau Region, Washington

    International Nuclear Information System (INIS)

    Leonhart, L.S.

    1979-07-01

    The Washington State portion of the Columbia Plateau is divided into six hydrologic sub-basins on the basis of the principal surface drainage systems present, structural and topographic relationships, and political and other considerations. Baseline descriptions of the surface water systems and resources are presented for the Columbia Plateau with emphasis on the Pasco Sub-basin. A preliminary evaluation of the hydrologic budget for each sub-basin is derived. For each sub-basin, recharge/discharge relationships arising from precipitation/evapotranspiration/runoff, stream losses and gains, and artificial mechanisms are determined on the basis of available data. The net exchange between surface and groundwater systems is evaluated and relative estimates of the net groundwater flow into or out of the sub-basin are obtained. An evaluation is made of hydrologic risk factors arising from: (1) tributary flooding in eastern Washington; and, (2) major flooding of the Columbia River within the Pasco Sub-basin. Scenarios are presented for credible natural and man-generated catastrophic events

  12. Deep long-period earthquakes beneath Washington and Oregon volcanoes

    Science.gov (United States)

    Nichols, M.L.; Malone, S.D.; Moran, S.C.; Thelen, W.A.; Vidale, J.E.

    2011-01-01

    Deep long-period (DLP) earthquakes are an enigmatic type of seismicity occurring near or beneath volcanoes. They are commonly associated with the presence of magma, and found in some cases to correlate with eruptive activity. To more thoroughly understand and characterize DLP occurrence near volcanoes in Washington and Oregon, we systematically searched the Pacific Northwest Seismic Network (PNSN) triggered earthquake catalog for DLPs occurring between 1980 (when PNSN began collecting digital data) and October 2009. Through our analysis we identified 60 DLPs beneath six Cascade volcanic centers. No DLPs were associated with volcanic activity, including the 1980-1986 and 2004-2008 eruptions at Mount St. Helens. More than half of the events occurred near Mount Baker, where the background flux of magmatic gases is greatest among Washington and Oregon volcanoes. The six volcanoes with DLPs (counts in parentheses) are Mount Baker (31), Glacier Peak (9), Mount Rainier (9), Mount St. Helens (9), Three Sisters (1), and Crater Lake (1). No DLPs were identified beneath Mount Adams, Mount Hood, Mount Jefferson, or Newberry Volcano, although (except at Hood) that may be due in part to poorer network coverage. In cases where the DLPs do not occur directly beneath the volcanic edifice, the locations coincide with large structural faults that extend into the deep crust. Our observations suggest the occurrence of DLPs in these areas could represent fluid and/or magma transport along pre-existing tectonic structures in the middle crust. ?? 2010 Elsevier B.V.

  13. Quantifying the Causes and Propogation of the 2015 Washington Wildfires

    Science.gov (United States)

    Engel, R.; Marlier, M. E.; Lettenmaier, D. P.

    2017-12-01

    In the summer of 2015, Washington state experienced wildfires that burned over 450,000 ha, more than five times the average and more than three times the next-most severe fire season in the 30-year record. We examine the confluence of factors that led to the extreme fire season, and evaluate whether 2015 can be used as a predictor of possible future conditions that will be affected by climate warming. In previous work, we have found that 2015 was an extremely warm summer (nearly 1 degree C warmer than the previous year in the 30-year record) but was not particularly anomalous in terms of many other climatic indicators, including reconstructed soil moisture, the Palmer Drought Severity Index (PDSI), and the Canadian Fire Weather Index. However, according to the Dead Fuel Moisture (DFM), a drying index used by the US Forest Service, 2015 was an extreme year of record. The DFM relies on temperature, precipitation, and relative humidity to establish a daily equilibrium moisture content of dead material. We examine both Washington's 2015 fire season and the 30-year fire record with respect to climatology and other potential drivers of fire (e.g. forest health, ignition). Additionally, we explore the role of land cover with respect to fire propagation through the season. While too many potential causes of extreme fires exist to establish a concrete long-term relationship at such a fine scale, we find that the 2015 fire anomaly was at least partially climatically driven.

  14. Washington's marine oil spill compensation schedule - simplified resource damage assessment

    International Nuclear Information System (INIS)

    Geselbracht, L.; Logan, R.

    1993-01-01

    The Washington State Preassessment Screening and Oil Spill Compensation Schedule Rule (Chapter 173-183 Washington Administrative Code), which simplifies natural resource damage assessment for many oil spill cases, became effective in May 1992. The approach described in the rule incorporates a number of preconstructed rankings that rate environmental sensitivity and the propensity of spilled oil to cause environmental harm. The rule also provides guidance regarding how damages calculated under the schedule should be reduced to take into account actions taken by the responsible party that reduce environmental injury. To apply the compensation schedule to marine estuarine spills, the resource trustees need only collect a limited amount of information such as type of product spilled, number of gallons spilled, compensation schedule subregions the spill entered, season of greatest spill impact, percent coverage of habitats affected by the spill, and actions taken by the responsible party. The result of adding a simplified tool to the existing assortment of damage assessment approaches is that resource trustees will now be able to assess damages for most oil spill cases and shift more effort than was possible in the past to resource restoration

  15. Surface hydrologic investigations of the Columbia Plateau region, Washington

    International Nuclear Information System (INIS)

    Leonhart, L.S.

    1979-01-01

    The Washington State portion of the Columbia Plateau is divided into six hydrologic sub-basins on the basis of the principal surface drainage systems present, structural and topographic relationships, and political and other considerations. Baseline descriptions of the surface water systems and resources are presented for the Columbia Plateau with emphasis on the Pasco Sub-basin. A preliminary evaluation of the hydrologic budget for each sub-basin is derived. For each sub-basin, recharge/discharge relationships arising from precipitation/evapotranspiration/runoff, stream losses and gains, and artificial mechanisms are determined on the basis of available data. The net exchange between surface and ground-water systems is evaluated and relative estimates of the net ground-water flow into or out of the sub-basin are obtained. An evaluation is made of hydrologic risk factors arising from: (1) tributary flooding in eastern Washington; and (2) major flooding of the Columbia River within the Pasco Sub-basin. Scenarios are presented for credible natural and man-generated catastrophic events

  16. Ergonomics and regulatory politics: the Washington State case.

    Science.gov (United States)

    Silverstein, Michael

    2007-05-01

    Every year in the State of Washington more than 50,000 workers experience a work related musculoskeletal disorder (WMSD), making up more than 30% of all worker compensation cases. In 2000, the Washington State Department of Labor and Industries (L&I) adopted a workplace ergonomics rule requiring employers to reduce worker exposure to hazards that cause or contribute to WMSDs. In 2003, the ergonomics rule was repealed by a margin of 53.5-46.5 in a statewide voter initiative. The official rulemaking record of approximately 100,000 pages, along with supplementary published and unpublished material, was reviewed. The relationship between scientific deliberation and the public policy process in adopting and repealing the ergonomics rule was assessed and described. The deliberative features of the regulatory, judicial, legislative, and ballot processes were compared. The ergonomics rule was successful in the regulatory and legal arenas where the process was most transparent and open to public involvement, differing views could be presented fully, and decision makers were expected to explain their decisions in light of the record. The rule fared most poorly in the legislature and at the ballot box when these features were lost and where considered deliberation was replaced by unconstrained political conflict. Additional checks and balances are needed.

  17. Basic Fibroblast Growth Factor Fused with Tandem Collagen-Binding Domains from Clostridium histolyticum Collagenase ColG Increases Bone Formation

    Directory of Open Access Journals (Sweden)

    Hiroyuki Sekiguchi

    2018-01-01

    Full Text Available Basic fibroblast growth factor 2 (bFGF accelerates bone formation during fracture healing. Because the efficacy of bFGF decreases rapidly following its diffusion from fracture sites, however, repeated dosing is required to ensure a sustained therapeutic effect. We previously developed a fusion protein comprising bFGF, a polycystic kidney disease domain (PKD; s2b, and collagen-binding domain (CBD; s3 sourced from the Clostridium histolyticum class II collagenase, ColH, and reported that the combination of this fusion protein with a collagen-like peptide, poly(Pro-Hyp-Gly10, induced mesenchymal cell proliferation and callus formation at fracture sites. In addition, C. histolyticum produces class I collagenase (ColG with tandem CBDs (s3a and s3b at the C-terminus. We therefore hypothesized that a bFGF fusion protein containing ColG-derived tandem CBDs (s3a and s3b would show enhanced collagen-binding activity, leading to improved bone formation. Here, we examined the binding affinity of four collagen anchors derived from the two clostridial collagenases to H-Gly-Pro-Arg-Gly-(Pro-Hyp-Gly12-NH2, a collagenous peptide, by surface plasmon resonance and found that tandem CBDs (s3a-s3b have the highest affinity for the collagenous peptide. We also constructed four fusion proteins consisting of bFGF and s3 (bFGF-s3, s2b-s3b (bFGF-s2b-s3, s3b (bFGF-s3b, and s3a-s3b (bFGF-s3a-s3b and compared their biological activities to those of a previous fusion construct (bFGF-s2b-s3 using a cell proliferation assay in vitro and a mouse femoral fracture model in vivo. Among these CB-bFGFs, bFGF-s3a-s3b showed the highest capacity to induce mesenchymal cell proliferation and callus formation in the mice fracture model. The poly(Pro-Hyp-Gly10/bFGF-s3a-s3b construct may therefore have the potential to promote bone formation in clinical settings.

  18. Detección de proteínas de adhesión a fibronectina y colágeno presentes en Leptospira interrogans serovar Canicola

    Directory of Open Access Journals (Sweden)

    Gisele Reyes

    2007-12-01

    Full Text Available Como parte de los estudios encaminados a la obtención de una formulación vacunal por subunidades contra la leptospirosis humana, se describe la purificación y caracterización de las proteínas de unión a fibronectina y a colágeno en Leptospira interrogans. Las proteínas de la membrana externa fueron extraídas mediante la solubilización con Tritón X-114 y se aplicaron en una columna de afinidad de IgG AntiBSA-Sepharose 2B CL, para eliminar la BSA, contaminante principal del medio de cultivo en que crece el microorganismo. La muestra libre de BSA (no fijado se aplicó a una columna de afinidad de fibronectina Sepharose 4B-CNBr, que permitió la separación y detección de una fracción que contenía una proteína de unión a fibronectina presente en la cepa 87 de Leptospira interrogans serovar Canicola, cuyo peso molecular fue estimado en 40 kDa. La proteína aislada demostró ser antigénica y conservada en los serovares Canicola, Copenhageni y Mozdok, en el ensayo de inmunodetección utilizado en este estudio (Dot blot. Para ello se utilizaron sueros específicos obtenidos en ratas infectadas experimentalmente con cada serovar y una mezcla de sueros de humanos convalecientes de leptospirosis. Las proteínas de membrana externa solubilizadas con Tritón X-114, libres de BSA, fueron aplicadas también a una columna de afinidad colágeno-Sepharosa 4B-CNBr, que permitió la purificación de una proteína de unión a colágeno con un peso molecular de aproximadamente 25 kDa, la cual resultó ser antigénica frente a sueros de humanos convalecientes de la enfermedad. Ambas proteínas seleccionadas (40 kD y 25 kD podrían ser evaluadas como posibles inmunógenos en futuros estudios encaminados a la obtención de nuevos antígenos vacunales.

  19. Alcohol Outlets and Violent Crime in Washington D.C.

    Directory of Open Access Journals (Sweden)

    Pan, William K

    2010-08-01

    Full Text Available Objective: Alcohol is more likely than any other drug to be involved in substance-related violence. In 2000 violence-related and self-directed injuries accounted for an estimated $37 billion and $33 billion in productivity losses and medical treatment, respectively. A review of emergency department data revealed violence and clinically identified trauma-related injuries have the strongest correlation among alcohol-dependent injuries. At the environmental level there is a relationship between alcohol outlet density and violent crime. A limited number of studies have examined the relationship between alcohol outlet type and the components of violent crime. The aim of this study is to examine the relationship between the aggregate components of violent crime and alcohol outlet density by type of outlet.Methods: For this study we used Washington, D.C. census tract data from the 2000 census to examine neighborhood characteristics. Alcohol outlet, violent crime, and population-level data for Washington, D.C. were drawn from various official yet publicly available sources. We developed an analytic database to examine the relationship between alcohol outlet category and four types of violent crime. After estimating spatial correlation and determining spatial dependence, we used a negative binomial regression analysis to assess the alcohol availability-violent crime association, while controlling for structural correlates of violence.Results: Independent of alternative structural correlates of violent crime, including the prevalence of weapons and illicit drugs, community-level alcohol outlet density is significantly associated with assaultive violence. Outlets were significantly related to robbery, assault, and sexual offenses. In addition, the relationship among on-premise and off-premise outlets varied across violent crime categories.Conclusion: In Washington, D.C., alcohol outlet density is significantly associated with the violent crimes. The

  20. História do Tempo Presente e América Latina: Colômbia - uma entrevista com Hugo Antonio Fazio Vengoa

    Directory of Open Access Journals (Sweden)

    Elisangela da Silva Machieski

    2017-05-01

    Full Text Available Hugo Antonio Fazio Vengoa é historiador e doutor em ciência política, professor da Universidad de los Andes (Bogotá – Colômbia desde a década de 1990, onde desenvolveu e coordenou diversas atividades, incluindo a criação dos programas de mestrado e doutorado em História. Atualmente é decano da Faculdade de Ciências Sociais. Suas investigações estão focadas em temas como a História Global, Estudos Internacionais Contemporâneos e História do Tempo Presente. No decorrer de sua carreira acadêmica publicou mais de vinte livros, dentre esses merece destaque o livro intitulado “La historia del tiempo presente: historiografía, problemas y métodos” (2010. Membro do Grupo de Investigação Historia del Tiempo Presente.

  1. Mineralogia, inclusões fluídas e gênese de esmeraldas das jazidas de Chivor, Coscuez, Muzo, Pacho e Yacopi, Colômbia

    OpenAIRE

    Fernando Heli Romero Ordóñez

    1993-01-01

    As jazidas de esmeralda da Colômbia estão localizadas na parte central da Cordilheira Oriental, nas regiões do Guavio e do Território Vásquez-Yacopí. Estas mineralizações de idade terciária, estão localizadas em rochas sedimentares marinhas de idade cretácea inferior, pertencentes às unidades estratigráficas Calizas del Guavio e Lutitas de Macanal, ambas situadas na região do Guavio, e às unidades Rosa Blanca e Paja localizadas no Território Vásquez-Yacopí. As características de posicionament...

  2. III Colóquio luso-brasileiro de educação a distância e elearning

    OpenAIRE

    Amante, Lúcia; Mendes, António Quintas; Barros, Daniela Melaré Vieira; Cardoso, Teresa; Spilker, Maria João

    2013-01-01

    O livro de atas do III Colóquio Luso-Brasileiro de Educação a Distância e Elearning, realizado em Lisboa em dezembro de 2013, reúne um vasto conjunto de comunicações apresentadas no evento e que se distribuem por 10 grandes temas a saber: EaD e Políticas educativas; Boas Práticas em Educação Online; Modelos e Processos Pedagógicos em Educação online; Web 2.0 e tecnologias emergentes; Pedagogias emergentes (PLEs, MOOCs, REAs, …); Realidade Virtual em EaD; Jogos e gamification em EaD; Mobile Le...

  3. Linkage of the gene that encodes the alpha 1 chain of type V collagen (COL5A1) to type II Ehlers-Danlos syndrome (EDS II).

    Science.gov (United States)

    Loughlin, J; Irven, C; Hardwick, L J; Butcher, S; Walsh, S; Wordsworth, P; Sykes, B

    1995-09-01

    Ehlers-Danlos syndrome (EDS) is a group of heritable disorders of connective tissue with skin, ligaments and blood vessels being the main sites affected. The commonest variant (EDS II) exhibits an autosomal dominant mode of inheritance and is characterized by joint hypermobility, cigarette paper scars, lax skin and excessive bruising. As yet no gene has been linked to EDS II, nor has linkage been established to a specific region of the genome. However, several candidate genes encoding proteins of the extracellular matrix have been excluded. Using an intragenic simple sequence repeat polymorphism, we report linkage of the COL5A1 gene, which encodes the alpha 1(V) chain of type V collagen, to EDS II. A maximum LOD score (Zmax) for linkage of 8.3 at theta = 0.00 was generated for a single large pedigree.

  4. Relatório sobre o colóquio Brasil-Japão: 25-27 de julho de 1966

    Directory of Open Access Journals (Sweden)

    Eurípedes Simões de Paula

    1966-09-01

    Full Text Available São Paulo, o mais expressivo núcleo urbano de influência japonesa em tôda a América Latina, abrigou, de 25 a 27 de julho de 1966, o I Colóquio Brasil-Japão. Nessa oportunidade encontraram-se professôres japonêses e brasileiros que juntos debateram temas culturais no campo de suas especialidades, cuja fecundidade pôde ser verificada pela vivacidade dos debates travados e pelo grande número de interessados que compareceram a tôdas as sessões realizadas no Departamento de História da Faculdade de Filosofia, Ciências e Letras da Universidade de São Paulo, situado na Cidade Universitária "Armando de Sales Oliveira, no Butantã, onde também funciona a Secção de Estudos Orientais da mesma Universidade.

  5. Effect of aging on the healing of colonic anastomoses in rats Efeitos do envelhecimento na cicatrização de anastomoses colônicas em ratos

    Directory of Open Access Journals (Sweden)

    Maria de Lourdes Pessole Biondo-Simões

    2004-04-01

    Full Text Available PURPOSE: To evaluate the healing of the colonic anastomoses in old and young adult rats. METHODS: Fifty six Wistar rats were allocated in two groups, the young animals aged on average 110 days and the old animals aged average 762 days. Under anesthesia, the rats underwent a midline laparotomy and the colon was sectioned 2 cm above the peritoneal deflection. An end-to-end interrupted one layer colonic anastomosis was performed and the animals sacrificed on the 3rd, 7th, 14th and 21st postoperative days. A 4 cm segment of colon containing the anastomosis was submitted to bursting pressure tests. The paraffin-embedded tissue blocs were sectioned and stained with haematoxylin and eosin and Sirius Red. Histochemical methods such as PCNA, LCA and CD34 were also used. RESULTS: The colonic anastomosis resistance was greater in the old rats group on the 3rd postoperative day (p=0,0000. Collagen concentration was higher in the anastomosis of the young animals on the 14th and 21st postoperative days (p=0,0475, p=0,0346 respectively, with a significantly greater concentration of collagen type I. The concentration of collagen type III, the epithelial lining rate and the angiogenesis were similar in the study groups. CONCLUSION: Despite some differences between the two groups regarding the healing process and considering that failures in the colonic anastomosis wound healing could not be demonstrated, we can concluded that aging itself does not interfere in the wound healing process.OBJETIVO: Avaliar a cicatrização de anastomoses colônicas em ratos adultos, jovens e velhos. MÉTODOS: Cinquenta e seis ratos machos, Wistar foram alocados em dois grupos, um de animais adultos jovens com idade media de 110 dias e outro de animais velhos, com idade media de 762 dias. Sob anesthesia os ratos forma submetidos a uma laparotomia mediana e o cólon foi seccionado 2 cm acima da reflexão peritoneal. Uma anastomose término-terminal foi feita e avaliada após 3, 7, 14

  6. High School Administrative Staffing in Washington State: Principal Perspectives on Resource Needs and Utilization

    Science.gov (United States)

    Steach, John C.

    2011-01-01

    This mixed methods study explored how high school principals prioritize their work and utilize available human resources to adjust to inadequate administrative staffing. Analysis of staffing levels across the state of Washington and specifically inside two eastern Washington districts framed interview questions for central office administration…

  7. 78 FR 25471 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

    Science.gov (United States)

    2013-05-01

    ....R50000] Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA..., Department of Anthropology, has completed an inventory of human remains and associated funerary objects, in... Burke Museum acting on behalf of the University of Washington, Department of Anthropology. Disposition...

  8. 78 FR 5200 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

    Science.gov (United States)

    2013-01-24

    ... Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA AGENCY: National... Anthropology, has completed an inventory of human remains and associated funerary objects, in consultation with... acting on behalf of the University of Washington, Department of Anthropology. Disposition of the human...

  9. 78 FR 19297 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

    Science.gov (United States)

    2013-03-29

    ...-PPWOCRADN0] Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA..., Department of Anthropology, has completed an inventory of human remains and associated funerary objects, in... Burke Museum acting on behalf of the University of Washington, Department of Anthropology. Disposition...

  10. 78 FR 19298 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

    Science.gov (United States)

    2013-03-29

    ...-PPWOCRADN0] Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA..., Department of Anthropology, has completed an inventory of human remains, in consultation with the appropriate... Washington, Department of Anthropology. Disposition of the human remains to the Indian tribes stated below...

  11. 78 FR 22286 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

    Science.gov (United States)

    2013-04-15

    ....R50000] Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA..., Department of Anthropology, has completed an inventory of human remains, in consultation with the appropriate... acting on behalf of the University of Washington, Department of Anthropology. If no additional requestors...

  12. 78 FR 5198 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

    Science.gov (United States)

    2013-01-24

    ... Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA AGENCY: National... Anthropology, has completed an inventory of human remains and associated funerary objects, in consultation with... of Washington, Department of Anthropology. Disposition of the human remains to the Indian tribes...

  13. 76 FR 73664 - Notice of Inventory Completion: Washington State University, Museum of Anthropology, Pullman, WA

    Science.gov (United States)

    2011-11-29

    ...: Washington State University, Museum of Anthropology, Pullman, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Washington State University, Museum of Anthropology (WSU) has completed an... University, Museum of Anthropology, Pullman, WA 99164-4910, telephone (509) 335-4314. SUPPLEMENTARY...

  14. An annotated checklist of the vascular flora of Washington County Mississippi

    Science.gov (United States)

    Field explorations have yielded 257 species new to Washington County, Mississippi and Calandrinia ciliata (Ruiz & Pav.) DC. and Ruellia nudiflora (Engelm. & Gray) Urban new to the state. An annotated list of 796 taxa for Washington County is provided and excludes 62 species that were reported from ...

  15. Integrated Digital English Acceleration (I-DEA). Washington's Community and Technical Colleges

    Science.gov (United States)

    Washington State Board for Community and Technical Colleges, 2015

    2015-01-01

    Washington state has a large and rapidly growing foreign-born population. In 2011, immigrants made up 16.5 percent of Washington's civilian employed workforce, up from 7.1 percent in 1990. These new arrivals create jobs by forming businesses, spending income in local economies and raising employers' productivity. Thanks to project I-DEA…

  16. Collection Development Policy: Federal Government Publications at Eastern Washington University Libraries.

    Science.gov (United States)

    Roselle, Ann; Chan, Karen

    This collection development policy serves as a guide for the selection and retention of depository government documents by the Government Publications Unit of the Kennedy Memorial Library of Eastern Washington University (EWU) in Cheney, Washington. The library selects approximately 65 percent of the depository items distributed by the U.S.…

  17. 78 FR 76031 - Sweet Cherries Grown in Designated Counties in Washington; Decreased Assessment Rate

    Science.gov (United States)

    2013-12-16

    ... FIR] Sweet Cherries Grown in Designated Counties in Washington; Decreased Assessment Rate AGENCY... subsequent fiscal periods from $0.18 to $0.15 per ton of sweet cherries handled. The Committee locally administers the marketing order for sweet cherries grown in designated counties in Washington. The Committee's...

  18. 77 FR 74871 - Notice of Inventory Completion: The Museum of Anthropology at Washington State University...

    Science.gov (United States)

    2012-12-18

    ... Inventory Completion: The Museum of Anthropology at Washington State University, Pullman, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Museum of Anthropology has completed an... objects may contact the Museum of Anthropology at Washington State University. Repatriation of the human...

  19. 78 FR 2432 - Notice of Inventory Completion: The Museum of Anthropology at Washington State University...

    Science.gov (United States)

    2013-01-11

    ... Inventory Completion: The Museum of Anthropology at Washington State University, Pullman, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Museum of Anthropology has completed an... objects may contact the Museum of Anthropology at Washington State University. Repatriation of the human...

  20. Integrated Basic Education and Skills Training (I-BEST). Washington's Community and Technical Colleges

    Science.gov (United States)

    Washington State Board for Community and Technical Colleges, 2014

    2014-01-01

    Washington's Integrated Basic Education and Skills Training Program (I-BEST) quickly teaches students literacy, work, and college-readiness skills so they can move through school and into living wage jobs faster. Pioneered by Washington's community and technical colleges, I-BEST uses a team-teaching approach to combine college-readiness classes…

  1. 78 FR 74163 - Harrison Medical Center, a Subsidiary of Franciscan Health System Bremerton, Washington; Notice...

    Science.gov (United States)

    2013-12-10

    ... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-83,070] Harrison Medical Center, a Subsidiary of Franciscan Health System Bremerton, Washington; Notice of Negative Determination... workers of Harrison Medical Center, a subsidiary of Franciscan Health System, Bremerton, Washington...

  2. 75 FR 10442 - Sweet Cherries Grown in Designated Counties in Washington; Change in the Handling Regulation

    Science.gov (United States)

    2010-03-08

    .... The existing paragraph (e) would be redesignated as paragraph (d), and the introductory sentence of... reported by the National Agricultural Statistics Service, USDA, and 1,500 Washington cherry producers, the... revise the introductory sentence of paragraph (g) to read as follows: Sec. 923.322 Washington cherry...

  3. Unmet Student Financial Need in the State of Washington: A Study of the "Need Gap."

    Science.gov (United States)

    Fenske, Robert; And Others

    A study of unmet student financial need in Washington State was conducted by the Washington Council for Postsecondary Education. "Unmet need" is the difference between need and the total amount of aid received by the student through federal, state, and institutional financial aid programs, privately funded scholarships, and nonsubsidized…

  4. 76 FR 18001 - Irish Potatoes Grown in Washington; Decreased Assessment Rate

    Science.gov (United States)

    2011-04-01

    ... IR] Irish Potatoes Grown in Washington; Decreased Assessment Rate AGENCY: Agricultural Marketing... rate established for the State of Washington Potato Committee (Committee) for the 2011-2012 and subsequent fiscal periods from $0.0035 to $0.003 per hundredweight of potatoes handled. The Committee locally...

  5. 78 FR 48285 - Irish Potatoes Grown in Washington; Decreased Assessment Rate

    Science.gov (United States)

    2013-08-08

    ...; FV13-946-1 FIR] Irish Potatoes Grown in Washington; Decreased Assessment Rate AGENCY: Agricultural... established for the State of Washington Potato Committee (Committee) for the 2013-2014 fiscal year and all subsequent fiscal periods from $0.003 to $0.0025 per hundredweight of potatoes handled. The Committee locally...

  6. 76 FR 41589 - Irish Potatoes Grown in Washington; Decreased Assessment Rate

    Science.gov (United States)

    2011-07-15

    ... FIR] Irish Potatoes Grown in Washington; Decreased Assessment Rate AGENCY: Agricultural Marketing... established for the State of Washington Potato Committee (Committee) for the 2011-2012 and subsequent fiscal periods from $0.0035 to $0.003 per hundredweight of potatoes handled. The Committee locally administers...

  7. A Grape Production Guide for Vocational Agriculture Instructors in Washington. Final Report.

    Science.gov (United States)

    Padelford, Stewart L.; Cvancara, Joseph G., Ed.

    This curriculum guide is intended to provide vocational agriculture instructors with an up-to-date resource dealing with grape production in Washington. Addressed in the individual units of the guide are the following topics: the history of grape production; grape types important to Washington; site selection for a vineyard; establishment and…

  8. 78 FR 78808 - Proposed Flood Elevation Determinations for Pierce County, Washington, and Incorporated Areas

    Science.gov (United States)

    2013-12-27

    ...-2013-0002; Internal Agency Docket No. FEMA-B-7748] Proposed Flood Elevation Determinations for Pierce... proposed rule concerning proposed flood elevation determinations for Pierce County, Washington, and... sources in Pierce County, Washington. On April 16, 2012, FEMA published a proposed rulemaking at 77 FR...

  9. A Partnership for Modeling the Marine Environment of Puget Sound, Washington

    Science.gov (United States)

    2009-03-30

    Northwest National Marine Renewable Energy Center, a joint University of Washington - Oregon State project funded by the U.S. Department of Energy. e. A... Marine Renewable Energy Center (NNMREC), a joint Washington - Oregon State project to investigate extraction of wave and tidal energy sponsored by

  10. The Impact of Interstate Migration on Human Capital Development in Washington

    Science.gov (United States)

    Spaulding, Randy

    2010-01-01

    Washington State is a leader in the innovation economy largely due to the combination of aerospace, software, and biomedical industries centered in the greater Seattle area; and, the state's high level of international trade. Despite Washington's national ranking, the state is overly reliant on importing educated workers from other states and…

  11. 78 FR 45958 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2013-07-30

    ... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-13406; PPWOCRADN0-PCU00RP14.R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke...

  12. 78 FR 59963 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2013-09-30

    ... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-13770; PPWOCRADN0-PCU00RP14.R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of..., Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum, University of...

  13. 78 FR 44595 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2013-07-24

    ... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-13407; PPWOCRADN0-PCU00RP14.R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke...

  14. 78 FR 17229 - Notice of Public Meeting, Eastern Washington Resource Advisory Council

    Science.gov (United States)

    2013-03-20

    ... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLORW00000 L102000000.ML0000 13XL1109AF.HAG13-0139] Notice of Public Meeting, Eastern Washington Resource Advisory Council AGENCY: Bureau of... the Eastern Washington Resource Advisory Council. The meeting on March 21, 2013, has been cancelled...

  15. Booker T. Washington's Educational Contributions to Contemporary Practices of Sustainable Development

    Science.gov (United States)

    Grant, Brett G.

    2014-01-01

    This article discusses Booker T. Washington's educational contributions to contemporary practices of sustainable development. In particular, the article looks at Washington's contributions in the areas of economic sustainability and entrepreneurship, character development, and aesthetics. As states continue to contemplate and evaluate the value of…

  16. Improving Immunization Coverage in a Rural School District in Pierce County, Washington

    Science.gov (United States)

    Peterson, Robin M.; Cook, Carolyn; Yerxa, Mary E.; Marshall, James H.; Pulos, Elizabeth; Rollosson, Matthew P.

    2012-01-01

    Washington State has some of the highest percentages of school immunization exemptions in the country. We compared school immunization records in a rural school district in Pierce County, Washington, to immunization records in the state immunization information system (IIS) and parent-held records. Correcting school immunization records resulted…

  17. 78 FR 50099 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

    Science.gov (United States)

    2013-08-16

    ... at the address in this notice by September 16, 2013. ADDRESSES: Alicia Woods, Washington State Parks... Alicia[email protected] . SUPPLEMENTARY INFORMATION: Notice is here given in accordance with the Native... request Alicia Woods, Washington State Parks and Recreation Commission, PO Box 42650, Olympia, WA 98504...

  18. 78 FR 44593 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

    Science.gov (United States)

    2013-07-24

    ...: Alicia Woods, Washington State Parks and Recreation Commission, PO Box 42650, Olympia, WA 98504-2650, telephone (360) 902- 0939, email Alicia[email protected] . SUPPLEMENTARY INFORMATION: Notice is here given... written request with information in support of the request to Alicia Woods, Washington State Parks and...

  19. 78 FR 44594 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

    Science.gov (United States)

    2013-07-24

    ..., 2013. ADDRESSES: Alicia Woods, Washington State Parks and Recreation Commission, PO Box 42650, Olympia, WA 98504-2650, telephone (360) 902- 0939, email Alicia[email protected] . SUPPLEMENTARY INFORMATION... to Alicia Woods, Washington State Parks and Recreation Commission, PO Box 42650, Olympia, WA 98504...

  20. An RNA-splicing mutation (G{sup +51VS20}) in the Type II collagen gene (COL2A1) in a family with spondyloepiphyseal dysplasia congenita

    Energy Technology Data Exchange (ETDEWEB)

    Tiller, G.E.; Polumbo, P.A. [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Weis, M.A.; Eyre, D.R. [Univ. of Washington, Seattle, WA (United States); Gruber, H.E.; Rimoin, D.L.; Cohn, D.H. [Cedars-Sinai Medical Center, Los Angeles, CA (United States)]|[Univ. of California School of Medicine, Los Angeles, CA (United States)

    1995-02-01

    Defects in type II collagen have been demonstrated in a phenotypic continuum of chondrodysplasias that includes achondrogenesis II, hypochondrogenesis, spondyloepiphyseal dysplasia congenita (SEDC), Kniest dysplasia, and Stickler syndrome. We have determined that cartilage from a terminated fetus with an inherited form of SEDC contained both normal {alpha}1(II) collagen chains and chains that lacked amino acids 256-273 of the triple-helical domain. PCR amplification of this region of COL2A1, from genomic DNA, yielded products of normal size, while amplification of cDNA yielded a normal sized species and a shorter fragment missing exon 20. Sequence analysis of genomic DNA from the fetus revealed a G{yields}T transversion at position +5 of intron 20; the affected father was also heterozygous for the mutation. Allele-specific PCR and heteroduplex analysis of a VNTR in COL2A1 independently confirmed the unaffected status of a fetus in a subsequent pregnancy. Thermodynamic calculations suggest that the mutation prevents normal splicing of exon 20 by interfering with binding of U{sub 1} small-nuclear RNA to pre-mRNA, thus leading to skipping of exon 20 in transcripts from the mutant allele. Electron micrographs of diseased cartilage showed intracellular inclusion bodies, which were stained by an antibody to {alpha}1(II) procollagen. Our findings support the hypothesis that {alpha}-chain length alterations that preserve the Gly-X-Y repeat motif of the triple helix result in partial intracellular retention of {alpha}1(II) procollagen and produce mild to moderate chondrodysplasia phenotypes. 50 refs., 6 figs., 1 tab.

  1. Custeio Baseado em Atividades (ABC Aplicado ao Setor Público: Estudo de Caso no Colégio Militar do Rio de Janeiro (CMRJ

    Directory of Open Access Journals (Sweden)

    Luis Paulo Faria Carmo, Lino Martins Silva

    2011-04-01

    Full Text Available O objetivo desta pesquisa foi verificar se o método de Custeio Baseado em Atividades (ABC é adequado para ser aplicado na mensuração dos custos educacionais no Colégio Militar do Rio de Janeiro. Embora a Lei federal nº. 4.320/64 já determinasse a necessidade de apuração dos custos, ingressos e resultados, a Administração Pública ainda não sabe quanto custa cada uma de suas atividades. A relevância do tema deriva da necessidade de as Organizações Públicas de permitir o acompanhamento e a avaliação de sua gestão. Porém, o Sistema Integrado de Administração Financeira do Governo Federal (SIAFI, principal ferramenta do governo, não evidencia em suas contas o custo de cada atividade. A metodologia utilizada teve como “principal finalidade desenvolver, esclarecer e modificar idéias a fim de fornecer hipótese pesquisável para estudos posteriores” (Tripodi, Fellin e Meyer, 1981, p.64 e como fontes de pesquisa a revisão bibliográfica e a análise documental, sendo utilizado como estratégia metodológica de um estudo de caso real no Colégio Militar do Rio de Janeiro (CMRJ. A análise dos resultados aponta ser adequada a aplicação do método de Custeio Baseado em Atividades (ABC para evidenciação dos custos das atividades educacionais, bem como subsidiar a avaliação da qualidade do gasto Público.

  2. Osteogenesis imperfecta type I: second-trimester diagnosis and incidental identification of a dominant COL1A1 deletion mutation in the paucisymptomatic father.

    Science.gov (United States)

    Chen, Chih-Ping; Su, Yi-Ning; Chang, Tung-Yao; Chern, Schu-Rern; Chen, Chen-Yu; Su, Jun-Wei; Wang, Wayseen

    2012-06-01

    To present second-trimester ultrasound and molecular diagnosis for osteogenesis imperfecta (OI) type I in a female fetus and incidental identification of a dominant COL1A1 deletion mutation in her paucisymptomatic father. A 30-year-old, primigravid woman was referred for genetic counseling in the second trimester because of bowing of the fetal lower limbs. She and her husband were non-consanguineous, and there was no family history of skeletal dysplasias. Prenatal ultrasound at 22 weeks of gestation revealed short and curved right femur and left tibia, and a short left fibula. The lengths of other long bones were normal. The husband was 158 cm tall, had blue sclerae, a history of habitual subluxation and dislocation of bilateral elbows and left knee, and an episode of left ulna fracture, and was not aware of his being affected with OI type I. The woman underwent amniocentesis. Cytogenetic analysis revealed a karyotype of 46,XX. Molecular analysis of the amniocytes revealed a heterozygous deletion mutation of c.1064_1068delCTGGT in exon 17 of the COL1A1 gene. By genetic testing the husband was found to carry the same mutation. Despite counseling of favorable outcome for OI type I with the parents, the woman elected to terminate the pregnancy. Postnatal skeletal X-ray findings were consistent with OI type I. Prenatal ultrasound diagnosis of mild forms of OI should include molecular analysis of type I collagen genes in both fetus and parents. Molecular genetic analysis of the family may incidentally identify a collagen gene mutation in the paucisymptomatic affected parent. Copyright © 2012. Published by Elsevier B.V.

  3. Deferoxamine Suppresses Collagen Cleavage and Protease, Cytokine, and COL10A1 Expression and Upregulates AMPK and Krebs Cycle Genes in Human Osteoarthritic Cartilage

    Directory of Open Access Journals (Sweden)

    Elena V. Tchetina

    2016-01-01

    Full Text Available This study reports the effects of the iron chelator deferoxamine (DFO on collagen cleavage, inflammation, and chondrocyte hypertrophy in relation to energy metabolism-related gene expression in osteoarthritic (OA articular cartilage. Full-depth explants of human OA knee articular cartilage from arthroplasty were cultured with exogenous DFO (1–50 μM. Type II collagen cleavage and phospho-adenosine monophosphate-activated protein kinase (pAMPK concentrations were measured using ELISAs. Gene expression studies employed real-time PCR and included AMPK analyses in PBMCs. In OA explants collagen cleavage was frequently downregulated by 10–50 μM DFO. PCR analysis of 7 OA patient cartilages revealed that 10 μM DFO suppressed expression of MMP-1, MMP-13, IL-1β, and TNFα and a marker of chondrocyte hypertrophy, COL10A1. No changes were observed in the expression of glycolysis-related genes. In contrast, expressions of genes associated with the mitochondrial Krebs cycle (TCA, AMPK, HIF1α, and COL2A1 were upregulated. AMPK gene expression was reduced in OA cartilage and increased in PBMCs from the same patients compared to healthy controls. Our studies demonstrate that DFO is capable of suppressing excessive collagenase-mediated type II collagen cleavage in OA cartilage and reversing phenotypic changes. The concomitant upregulation of proanabolic TCA-related gene expressions points to a potential for availability of energy generating substrates required for matrix repair by end-stage OA chondrocytes. This might normally be prevented by high whole-body energy requirements indicated by elevated AMPK expression in PBMCs of OA patients.

  4. Avaliação de substratos para reprodução de colêmbolos nativos em condições de laboratório.

    Directory of Open Access Journals (Sweden)

    Ricardo Bemfica Steffen

    2010-08-01

    Full Text Available Estudos utilizando a fauna edáfica como bioindicador da qualidade do solo têm sido de grande importância para entender as variações físicas, químicas e biológicas de ecossistemas. Dentre os organismos estudados, os colêmbolos constituem um dos principais grupos. Este trabalho teve como objetivo avaliar a multiplicação de colêmbolos nativos em condições de laboratório. Como substrato, utilizaram-se amostras de solo coletadas em área de citrus e bosque de pinus, com os tratamentos: a amostra de solo de área de cítrus com palha de ervilhaca moída, palha de milho moída, palha de nabo moída, cama de aviário moída e amostra de solo e, b em solo de bosque de pinus com: palha de ervilhaca moída, palha de milho moída, palha de nabo moída, cama de aviário moída e amostra de solo. Os tratamentos foram mantidos em potes plásticos, a 26OC, no escuro, sendo realizadas cinco avaliações durante o período de 180 dias. O solo de pomar de cítrus com adição de palha de nabo moído favoreceu a reprodução destes organismos em condições de laboratório.

  5. Natural phenomena hazards, Hanford Site, south central Washington

    International Nuclear Information System (INIS)

    Tallman, A.M.

    1996-01-01

    This document presents the natural phenomena hazard (NPH) loads for use in implementing DOE Order 5480.28, Natural Phenomena Hazards Mitigation, at the Hanford Site in south-central Washington State. The purpose of this document is twofold: (1) summarize the NPH that are important to the design and evaluation of structures, systems, and components at the Hanford Site; (2) develop the appropriate natural phenomena loads for use in the implementation of DOE Order 5480.28. The supporting standards, DOE-STD-1020-94, Natural Phenomena Hazards Design and Evaluation Criteria for Department of Energy Facilities (DOE 1994a); DOE-STD-1022-94, Natural Phenomena Hazards Site Characteristics Criteria (DOE 1994b); and DOE-STD-1023-95, Natural Phenomena Hazards Assessment Criteria (DOE 1995) are the basis for developing the NPH loads

  6. Environmental assessment overview, Reference repository location, Hanford site, Washington

    International Nuclear Information System (INIS)

    1986-05-01

    In February 1983, the US Department of Energy (DOE) identified a reference repository location at the Hanford Site in Washington as one of the nine potentially acceptable sites for a mined geologic repository for spent nuclear fuel and high-level radioactive waste. The site is in the Columbia Plateau, which is one of five distinct geohydrologic settings considered for the first repository. To determine their suitability, the Hanford site and the eight other potentially acceptable sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. On the basis of the evaluations reported in this EA, the DOE has found that the Hanford site is not disqualified under the guidelines. On the basis of these findings, the DOE is nominating the Hanford site as one of five sites suitable for characterization. 3 figs

  7. Cigarette price variation around high schools: evidence from Washington DC.

    Science.gov (United States)

    Cantrell, Jennifer; Ganz, Ollie; Anesetti-Rothermel, Andrew; Harrell, Paul; Kreslake, Jennifer M; Xiao, Haijun; Pearson, Jennifer L; Vallone, Donna; Kirchner, Thomas R

    2015-01-01

    This study examines lowest cigarette prices in all tobacco retail outlets in Washington D.C. (n=750) in relation to the type and number of high schools nearby, controlling for confounders. The lowest overall and Newport menthol prices were significantly lower at outlets near public non-charter and charter schools compared with outlets near private schools. Given higher smoking prevalence and more price-sensitive youth subgroups in U.S. public schools, exposure to low prices may contribute to tobacco-related health disparities in minority and low-income populations. Tobacco taxes combined with policies to minimize the increasing use of price as a marketing tool are critical. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Comparing Measures of Late HIV Diagnosis in Washington State

    Directory of Open Access Journals (Sweden)

    Laura Saganic

    2012-01-01

    Full Text Available As more US HIV surveillance programs routinely use late HIV diagnosis to monitor and characterize HIV testing patterns, there is an increasing need to standardize how late HIV diagnosis is measured. In this study, we compared two measures of late HIV diagnosis, one based on time between HIV and AIDS, the other based on initial CD4+ results. Using data from Washington's HIV/AIDS Reporting System, we used multivariate logistic regression to identify predictors of late HIV diagnosis. We also conducted tests for trend to determine whether the proportion of cases diagnosed late has changed over time. Both measures lead us to similar conclusions about late HIV diagnosis, suggesting that being male, older, foreign-born, or heterosexual increase the likelihood of late HIV diagnosis. Our findings reaffirm the validity of a time-based definition of late HIV diagnosis, while at the same time demonstrating the potential value of a lab-based measure.

  9. University of Washington, Nuclear Physics Laboratory annual report, 1995

    International Nuclear Information System (INIS)

    1995-04-01

    The Nuclear Physics Laboratory of the University of Washington supports a broad program of experimental physics research. The current program includes in-house research using the local tandem Van de Graff and superconducting linac accelerators and non-accelerator research in double beta decay and gravitation as well as user-mode research at large accelerator and reactor facilities around the world. This book is divided into the following areas: nuclear astrophysics; neutrino physics; nucleus-nucleus reactions; fundamental symmetries and weak interactions; accelerator mass spectrometry; atomic and molecular clusters; ultra-relativistic heavy ion collisions; external users; electronics, computing, and detector infrastructure; Van de Graff, superconducting booster and ion sources; nuclear physics laboratory personnel; degrees granted for 1994--1995; and list of publications from 1994--1995

  10. GOAT ROCKS WILDERNESS AND ADJACENT ROADLESS AREAS, WASHINGTON.

    Science.gov (United States)

    Church, S.E.; Close, T.J.

    1984-01-01

    The Goat Rocks Wilderness and adjacent roadless areas are a rugged, highly forested, scenic area located on the crest of the Cascade Range in south-central Washington. Several mineral claims have been staked in the area. Mineral surveys were conducted. Geochemical, geophysical, and geologic investigations indicate that three areas have probable mineral-resource potential for base metals in porphyry-type deposits. Available data are not adequate to permit definition of the potential for oil and gas. There is little likelihood for the occurrence of other kinds of energy resources in the area. Evaluation of resource potential in the three areas identified as having probable mineral-resource potential could be improved by more detailed geochemical studies and geologic mapping.

  11. Safety of union home care aides in Washington State.

    Science.gov (United States)

    Schoenfisch, Ashley L; Lipscomb, Hester; Phillips, Leslie E

    2017-09-01

    A rate-based understanding of home care aides' adverse occupational outcomes related to their work location and care tasks is lacking. Within a 30-month, dynamic cohort of 43 394 home care aides in Washington State, injury rates were calculated by aides' demographic and work characteristics. Injury narratives and focus groups provided contextual detail. Injury rates were higher for home care aides categorized as female, white, 50 to working through an agency (versus individual providers). In addition to direct occupational hazards, variability in workload, income, and supervisory/social support is of concern. Policies should address the roles and training of home care aides, consumers, and managers/supervisors. Home care aides' improved access to often-existing resources to identify, manage, and eliminate occupational hazards is called for to prevent injuries and address concerns related to the vulnerability of this needed workforce. © 2017 Wiley Periodicals, Inc.

  12. Geologic History of Eocene Stonerose Fossil Beds, Republic, Washington, USA

    Directory of Open Access Journals (Sweden)

    George E. Mustoe

    2015-07-01

    Full Text Available Eocene lakebed sediments at Stonerose Interpretive Center in Republic, Washington, USA are one of the most important Cenozoic fossil sites in North America, having gained international attention because of the abundance and diversity of plant, insect, and fish fossils. This report describes the first detailed geologic investigation of this unusual lagerstätten. Strata are gradationally divided into three units: Siliceous shale that originated as diatomite, overlain by laminated mudstone, which is in turn overlain by massive beds of lithic sandstone. The sedimentary sequence records topographic and hydrologic changes that caused a deep lake to become progressively filled with volcaniclastic detritus from earlier volcanic episodes. The location of the ancient lake within an active graben suggests that displacements along the boundary faults were the most likely trigger for changes in depositional processes.

  13. Regional basalt hydrology of the Columbia Plateau in Washington

    International Nuclear Information System (INIS)

    Tanaka, H.; Barrett, G.; Wildrick, L.

    1979-10-01

    This study is part of the Basalt Waste Isolation Project, operated for the US Department of Energy by Rockwell Hanford Operations. The overall purpose of the study is to assess locations within the Columbia River Basalt Group beneath the Hanford Site in south-central Washington suitable for a geologic repository for radioactive waste. This hydrologic study was made to describe the hydrologic characteristics of the basalt units of the Columbia Plateau. This was done by comprehensive data compilation, data interpretation and analysis. Data are presented in the form of maps and tables suitable as input information about the regional hydrology for possible future analysis by computer models. The report includes: an introduction; basic data; interpretation which covers stratigraphic trend surface, water levels, transmissivity and storage of aquifers, recharge, discharge, flow, subbasins, cross sections, references and appendix of record of wells

  14. Nuclear Physics Laboratory, University of Washington annual report

    International Nuclear Information System (INIS)

    1998-04-01

    The Nuclear Physics Laboratory at the University of Washington in Seattle pursues a broad program of nuclear physics. These activities are conducted locally and at remote sites. The current programs include in-house research using the local tandem Van de Graaff and superconducting linac accelerators and non-accelerator research in solar neutrino physics at the Sudbury Neutrino Observatory in Canada and at SAGE in Russia, and gravitation as well as user-mode research at large accelerators and reactor facilities around the world. Summaries of the individual research projects are included. Areas of research covered are: fundamental symmetries, weak interactions and nuclear astrophysics; neutrino physics; nucleus-nucleus reactions; ultra-relativistic heavy ions; and atomic and molecular clusters

  15. Cascade Apartments - Deep Energy Multifamily Retrofit , Kent, Washington (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2014-02-01

    In December of 2009-10, King County Housing Authority (KCHA) implemented energy retrofit improvements in the Cascade multifamily community, located in Kent, Washington (marine climate.)This research effort involved significant coordination from stakeholders KCHA, WA State Department of Commerce, utility Puget Sound Energy, and Cascade tenants. This report focuses on the following three primary BA research questions : 1. What are the modeled energy savings using DOE low income weatherization approved TREAT software? 2. How did the modeled energy savings compare with measured energy savings from aggregate utility billing analysis? 3. What is the Savings to Investment Ratio (SIR) of the retrofit package after considering utility window incentives and KCHA capitol improvement funding.

  16. Physician Assistants and Nurse Practitioners in Rural Washington Emergency Departments.

    Science.gov (United States)

    Nelson, Scott C; Hooker, Roderick S

    2016-06-01

    One role of physician assistants (PAs) and nurse practitioners (NPs) is to meet the growing demand for access to rural health care. Critical Access Hospitals, those with less than 25 beds, are usually located in rural communities, often providing continuity of care that clinics cannot deliver. Because little is known about staffing in these small hospital emergency departments, an exploratory study was undertaken using a mixed-methods approach. In Washington State, 18 of the 39 Critical Access Hospitals staff their emergency departments with PAs and NPs. Utilization data were collected through structured interviews by phone or in person on site. Most PAs and NPs lived within the community and staffing tended to be either 24 hours in-house or short notice if they lived or worked nearby. Emergency department visits ranged from 200 to 25,000 per year. All sites were designated level V or IV trauma centers and often managed cardiac events, significant injuries and, in some larger settings, obstetrics. In most instances, PAs were the sole providers in the emergency departments, albeit with physician backup and emergency medical technician support if a surge of emergency cases arose. Two-thirds of the PAs had graduated within the last 5 years. Most preferred the autonomy of the emergency department role and all expressed job satisfaction. Geographically, the more remote a Washington State Critical Access Hospital is, the more likely it will be staffed by PAs/NPs. The diverse utilization of semiautonomous PAs and NPs and their rise in rural hospital employment is a new workforce observation that requires broader investigation.

  17. A green roof grant program for Washington DC

    International Nuclear Information System (INIS)

    Johnson, P.A.

    2007-01-01

    The Chesapeake Bay Foundation (CBF) began its green roof demonstration project with $300,000 in funding provided by the DC Water and Sewer Authority. This paper reviewed the history of the project, its goals and early findings. The main objective was to demonstrate the technical, policy and economic feasibility of installing green roofs on commercial buildings in Washington DC and to promote green roofs as a means to manage storm water and improve water quality through the reduction of excessive runoff. The CBF has issued grants for the installation of 7 green roofs varying in size, design, location, and use. The projects included both new and existing structures designed to improve storm-water management in an urban area with significant pollution stress on the adjacent rivers. This paper provided technical, cost, and performance evaluations of each roof. A public outreach segment provided information to decision-makers to encourage more widespread replication of green roof technology throughout the metropolitan area. Much of the District of Columbia is served by a combined sewer system that becomes overloaded and discharges raw sewage into adjacent rivers during even moderately heavy rains. An average of 75 overflow events each year result in 1.5 billion gallons discharged into the Anacostia River. The installation of green roofs on buildings in the combined sewer area would retain storm water during these heavy rains and reduce the amount of overflow discharges. Apartments, as well as commercial and government buildings with mostly flat roofs are the most likely candidates for green roofs. The demonstration roofs are intended to become models, which all building owners could use as a guide for future plans for construction or re-construction to expand green roof coverage in Washington DC. It was emphasized that although such large-scale replication will take time and financial investments, it is achievable given enough political will and commercial awareness of

  18. The 1980-1982 Geothermal Resource Assessment Program in Washington

    Energy Technology Data Exchange (ETDEWEB)

    Korosec, Michael A.; Phillips, William M.; Schuster, J.Eric

    1983-08-01

    Since 1978, the Division of Geology and Earth Resources of the Washington Department of Natural Resources has participated in the U.S. Department of Energy's (USDOE) State-Coupled Geothermal Resource Program. Federal and state funds have been used to investigate and evaluate the potential for geothermal resources, on both a reconnaissance and area-specific level. Preliminary results and progress reports for the period up through mid-1980 have already been released as a Division Open File Report (Korosec, Schuster, and others, 1981). Preliminary results and progress summaries of work carried out from mid-1980 through the end of 1982 are presented in this report. Only one other summary report dealing with geothermal resource investigations in the state has been published. An Information Circular released by the Division (Schuster and others, 1978) compiled the geology, geochemistry, and heat flow drilling results from a project in the Indian Heaven area in the south Cascades. The previous progress report for the geothermal program (Korosec, Schuster, and others, 1981) included information on temperature gradients measured throughout the state, heat flow drilling in the southern Cascades, gravity surveys for the southern Cascades, thermal and mineral spring investigations, geologic mapping for the White Pass-Tumac Mountain area, and area specific studies for the Camas area of Clark County and Mount St. Helens. This work, along with some additional studies, led to the compilation of the Geothermal Resources of Washington map (Korosec, Kaler, and others, 1981). The map is principally a nontechnical presentation based on all available geothermal information, presented as data points, tables, and text on a map with a scale of 1:500,000.

  19. The power professorship program at Washington State University

    International Nuclear Information System (INIS)

    Mosher, C.C.; Shamash, Y.

    1993-01-01

    As with most electric power programs, Washington State University's has existed since the beginning of the engineering program 100 years ago. It has grown and developed largely through the efforts of a few dedicated individuals. The Power Professorship Program has existed since 1972. The Power Professor has been Dr. Clifford C. Mosher until his recent semi-retirement. The Power Professorship was conceived of as an avenue for joint university-industry interaction. Considerable time and ingenuity by visionary engineers and others have resulted in development of a financial base for the Power Professorship Program. The program has been funded equally by public and investor-utility sectors. Following financial difficulties stemming from the Washington Public Power Supply System financial default on public utility bonds for several nuclear projects, funding for the program from the public sector was canceled. After several lean years, public-sector support was again restored by WSU's electrical engineering department offering a contract for services to the utilities in exchange for funding. This contract has been renewed annually, with costs and benefits firmly established through careful analysis and consultation. Problems facing the power industry in the early 1970s with regard to establishing a pipeline of future human resources, were almost identical with those of the present time: indifferent feelings about the industry in general, students being attracted to more glamorous disciplines, and a decline in educational opportunities available in the power area. A 1985 article in the IEEE Power Engineering Review describing today's declining enrollment in power engineering applies equally well to previous periods. A major driving force for initiating utility-industry participation in the Power Professorship Program was the concern for maintaining a source of entry-level engineers with a background in power engineering

  20. Percutaneous injuries among dental professionals in Washington State

    Directory of Open Access Journals (Sweden)

    Shah Syed M

    2006-10-01

    Full Text Available Abstract Background Percutaneous exposure incidents facilitate transmission of bloodborne pathogens such as human immunodeficiency virus (HIV, hepatitis C virus (HCV and hepatitis B virus (HBV. This study was conducted to identify the circumstances and equipment related to percutaneous injuries among dental professionals. Methods We used workers' compensation claims submitted to the Department of Labor and Industries State Fund during a 7-year period (1995 through 2001 in Washington State for this study. We used the statement submitted by the injured worker on the workers' compensation claim form to determine the circumstances surrounding the injury including the type of activity and device involved. Results Of a total of 4,695 accepted State Fund percutaneous injury claims by health care workers (HCWs, 924 (20% were submitted by dental professionals. Out of 924 percutaneous injuries reported by dental professionals 894 (97% were among dental health care workers in non-hospital settings, including dentists (66, 7%, dental hygienists (61, 18% and dental assistants (667, 75%. The majority of those reporting were females (638, 71%. Most (781, 87% of the injuries involved syringes, dental instruments (77, 9%, and suture needles (23%. A large proportion (90% of injuries occurred in offices and clinics of dentists, while remainder occurred in offices of clinics and of doctors of medicine (9%, and a few in specialty outpatient facilities (1%. Of the 894 dental health care workers with percutaneous injuries, there was evidence of HBV in 6 persons, HCV in 30 persons, HIV in 3 persons and both HBV and HVC (n = 2 exposure. Conclusion Out of hospital percutaneous injuries are a substantial risk to dental health professionals in Washington State. Improved work practices and safer devices are needed to address this risk.

  1. The 16th International Geological Congress, Washington, 1933

    Science.gov (United States)

    Nelson, C.M.

    2009-01-01

    In 1933, the International Geological Congress (IGC) returned to the United States of America (USA) for its sixteenth meeting, forty-two years after the 5th IGC convened in Washington. The Geological Society of America and the U.S. Geological Survey (USGS) supplied the major part of the required extra-registration funding after the effects of the Great Depression influenced the 72th U.S. Congress not to do so. A reported 1, 182 persons or organizations, representing fifty-four countries, registered for the 16 th IGC and thirty-four countries sent 141 official delegates. Of the total number of registrants, 665 actually attended the meeting; 500 came from the USA; and fifteen had participated in the 5th IGC. The 16 th Meeting convened in the U.S. Chamber of Commerce Building from 22 to 29 July. The eighteen half-day scientific sections-orogenesis (four), major divisions of the Paleozoic (three), miscellaneous (three), batholiths and related intrusives (two), arid-region geomorphic processes and products (one), fossil man and contemporary faunas (one), geology of copper and other ore deposits (one), geology of petroleum (one), measuring geologic time (one), and zonal relations of metalliferous deposits (one)-included 166 papers, of which fifty (including several of the key contributions) appeared only by title. The Geological Society of Washington, the National Academy of Sciences, and the U.S. Bureau of Mines hosted or contributed to evening presentations or receptions. Twenty-eight of the 16th IGC's thirty new guidebooks and one new USGS Bulletin aided eight pre-meeting, seven during-meeting, and four post-meeting field trips of local, regional, or national scope. The remaining two new guidebooks outlined the USA's structural geology and its stratigraphic nomenclature. The 16th IGC published a two-volume monograph on the world's copper resources (1935) and a two-volume report of its proceedings (1936).

  2. ABNORMAL TYPE-III COLLAGEN PRODUCED BY AN EXON-17-SKIPPING MUTATION OF THE COL3A1 GENE IN EHLERS-DANLOS SYNDROME TYPE-IV IS NOT INCORPORATED INTO THE EXTRACELLULAR-MATRIX

    NARCIS (Netherlands)

    CHIODO, AA; SILLENCE, DO; COLE, WG; BATEMAN, JF

    1995-01-01

    A novel heterozygous mutation of the COL3Al gene that encodes the alpha 1(III) chains of type III collagen was identified in a family with the: acrogeric form of Ehlers-Danlos syndrome type IV (EDS-IV). Cultured dermal fibroblasts produced normal and shortened alpha 1(III) chains. The triple helix

  3. A novel zinc finger protein 219-like (ZNF219L) is involved in the regulation of collagen type 2 alpha 1a (col2a1a) gene expression in zebrafish notochord.

    Science.gov (United States)

    Lien, Huang-Wei; Yang, Chung-Hsiang; Cheng, Chia-Hsiung; Hung, Chin-Chun; Liao, Wei-Hao; Hwang, Pung-Pung; Han, Yu-San; Huang, Chang-Jen

    2013-01-01

    The notochord is required for body plan patterning in vertebrates, and defects in notochord development during embryogenesis can lead to diseases affecting the adult. It is therefore important to elucidate the gene regulatory mechanism underlying notochord formation. In this study, we cloned the zebrafish zinc finger 219-like (ZNF219L) based on mammalian ZNF219, which contains nine C2H2-type zinc finger domains. Through whole-mount in situ hybridization, we found that znf219L mRNA is mainly expressed in the zebrafish midbrain-hindbrain boundary, hindbrain, and notochord during development. The znf219L morpholino knockdown caused partial abnormal notochord phenotype and reduced expression of endogenous col2a1a in the notochord specifically. In addition, ZNF219L could recognize binding sites with GGGGG motifs and trigger augmented activity of the col2a1a promoter in a luciferase assay. Furthermore, in vitro binding experiments revealed that ZNF219L recognizes the GGGGG motifs in the promoter region of the zebrafish col2a1a gene through its sixth and ninth zinc finger domains. Taken together, our results reveal that ZNF219L is involved in regulating the expression of col2a1a in zebrafish notochord specifically.

  4. Tempo de reepitelização corneana com a instilação de colírio contendo hialuronato de sódio e carboximetilcelulose

    Directory of Open Access Journals (Sweden)

    Luciane Bugman Moreira

    2013-10-01

    Full Text Available OBJETIVO: Avaliar o tempo de reepitelização corneana pós abrasão usando colírios comercialmente disponíveis, um contendo hialuronato de sódio a 0,4%, outro contendo carboximetilcelulose a 1%, e comparar com a reepitelização sem instilação de colírio. MÉTODOS: Foram utilizados 24 coelhos, nos quais foi feita a abrasão mecânica da córnea nos 8 mm centrais. Esses animais foram divididos em três grupos. O primeiro grupo recebeu um colírio disponível comercialmente contendo hialuronato de sódio 0,4%, o segundo recebeu um colírio contendo carboximetilcelulose 1% e o terceiro não recebeu nenhuma droga. A avaliação foi feita a cada 24 horas por meio da análise de fotografias digitais sob luz azul de cobalto e coramento das córneas com fluoresceína a 2%. O estudo das imagens foi feito pelo sistema de análise de imagens do Autocad 2009®. A análise dos dados foi feita comparando o tempo total de reepitelização da córnea e a cada 24 horas entre os três grupos. RESULTADOS: A velocidade de reepitelização do grupo que usou colírio contendo hialuronato de sódio foi em média 90 horas; o grupo que usou carboximetilcelulose apresentou média de 105 horas; e o grupo que não usou nenhum tipo de lubrificante apresentou média de 108 horas para total reepitelização. Houve uma melhor performance na reepitelização após 96 horas nas córneas dos coelhos que usaram os colírios lubrificantes, sendo essa diferença estatisticamente comprovada. CONCLUSÃO: O colírio contendo hialuronato de sódio 0,4% mostrou índice de eficácia maior que aquele contendo carboximetilcelulose 1%, e este maior eficácia que o controle. Os resultados encontrados neste estudo mostram que o uso de lubrificantes no processo de reepitelização são de extrema valia e devem ser usados de rotina na clínica oftalmológica.

  5. Influence of aging on the quality of the skin of white women: the role of collagen Influência do envelhecimento na qualidade da pele de mulheres brancas: o papel do colágeno

    Directory of Open Access Journals (Sweden)

    Eloina do Rocio Valenga Baroni

    2012-10-01

    Full Text Available PURPOSE: Evaluate the influence of aging on the quality of the skin of white women, analyzing the dermal collagen. METHODS: Pre-auricular flaps were collected for histological and morphometric analysis of 218 white women who underwent spontaneous facial aesthetic plastic surgery. Picrosirius ultrared stain was used for analysis and quantification of collagen in five age groups (OBJETIVO: Avaliar a influência do envelhecimento na qualidade da pele de mulheres brancas analisando o colágeno dérmico. MÉTODOS: Realizou-se análise histológica e morfométrica de 218 retalhos pré-auriculares de mulheres brancas que se submeteram espontaneamente à cirurgia estética facial. Foi usada a coloração de Picrosirius Ultrared para analisar e quantificar os colágenos I, III e total em cinco grupos etários (<40 anos, 40 a 49 anos, 50 a 59 anos, 60 a 69 anos e 70 a 79 anos. RESULTADOS: A análise histológica mostrou alterações sugestivas de envelhecimento cutâneo (fragmentação e desorganização das fibras de colágeno, especialmente em pacientes acima de 60 anos. Não houve diferenças significantes entre a idade e a espessura da derme e da epiderme, mas houve diferenças significantes entre as percentagens de colágeno I, III e total (p<0,001 com o aumento da idade. CONCLUSÃO: Existe redução do colágeno com o aumento da idade e um aumento na sua degradação, levando à fragmentação das fibras.

  6. Avaliação e monitoramento de implantes ósseos de arcabouços de PLDLA imobilizados com colágeno, préviamente cultivados com osteoblastos

    Directory of Open Access Journals (Sweden)

    Paula Galhano Filler

    2014-10-01

    Full Text Available Introdução: Polímeros biorreabsorvíveis, como PLDLA, são amplamente empregados como arcabouços na engenharia tecidual óssea. Incentiva-se sua interação com macromoléculas naturais da matriz extracelular, como o colágeno, para melhor adesão e reconhecimento do material implantado, e maior neoformação óssea. Objetivo: Analisar e comparar a regeneração óssea através de implantes realizados na calota craniana de ratos. Metodologia: Utilizou-se ratos Wistar de aproximadamente 2 meses, e tempos de sacrifício de 8 e 12 semanas, divididos nos grupos: defeito vazio; PLDLA-Col sem osteoblastos; e PLDLA-Col com osteoblastos. Paralelamente, estudou-se a fluorescência PKH26 em animais de 4 semanas, para avaliar a capacidade dos arcabouços de suportar o crescimento celular in vitro, Resultados: A analise histológica dos tempos de 8 e 12 semanas demonstrou neoformação óssea acentuada e irregular nos implantes de PLDLA-Col com ou sem célula, e fechamento parcial da lesão na maioria dos casos. Além disso, observou-se crescimento ósseo sem relação com as bordas do defeito. A histomorfometria de ambos os tempos apresentou o seguinte resultado decrescente em relação a área óssea neoformada: tratamento > controle positivo > controle negativo. Conclusão: Arcabouços de PLDLA-Col, previamente ou não cultivados com osteoblastos, obtiveram resultados promissores quando comparados ao defeito vazio, confirmando sua importante utilidade na engenharia tecidual.

  7. 76 FR 28066 - Notice of Intent To Repatriate a Cultural Item: Museum of Anthropology at Washington State...

    Science.gov (United States)

    2011-05-13

    ... Cultural Item: Museum of Anthropology at Washington State University, Pullman, WA AGENCY: National Park... possession of the Museum of Anthropology at Washington State University, Pullman, WA, that meets the... notice. One lot of stone, bone, and glass beads was given to the Museum of Anthropology at Washington...

  8. 76 FR 14045 - Notice of Intent To Repatriate a Cultural Item: Museum of Anthropology at Washington State...

    Science.gov (United States)

    2011-03-15

    ...: Museum of Anthropology at Washington State University, Pullman, WA AGENCY: National Park Service... Museum of Anthropology at Washington State University, Pullman, WA, that meets the definition of..., a copper pendant was given to the Museum of Anthropology at Washington State University for intended...

  9. Clash of the Titans: Booker T. Washington and W. E. B. Du Bois. Curriculum Based Education Program, Grade 11.

    Science.gov (United States)

    National Park Service (Dept. of Interior), Washington, DC.

    The Booker T. Washington National Monument preserves and protects the birth site and childhood home of Booker T. Washington while interpreting his life experiences and significance in U.S. history as the most powerful African American between 1895 and 1915. The programs and activities included in this guide about the Booker T. Washington and W. E.…

  10. Geologic map of the Washington West 30’ × 60’ quadrangle, Maryland, Virginia, and Washington D.C.

    Science.gov (United States)

    Lyttle, Peter T.; Aleinikoff, John N.; Burton, William C.; Crider, E. Allen; Drake, Avery A.; Froelich, Albert J.; Horton, J. Wright; Kasselas, Gregorios; Mixon, Robert B.; McCartan, Lucy; Nelson, Arthur E.; Newell, Wayne L.; Pavlides, Louis; Powars, David S.; Southworth, C. Scott; Weems, Robert E.

    2018-01-02

    The Washington West 30’ × 60’ quadrangle covers an area of approximately 4,884 square kilometers (1,343 square miles) in and west of the Washington, D.C., metropolitan area. The eastern part of the area is highly urbanized, and more rural areas to the west are rapidly being developed. The area lies entirely within the Chesapeake Bay drainage basin and mostly within the Potomac River watershed. It contains part of the Nation's main north-south transportation corridor east of the Blue Ridge Mountains, consisting of Interstate Highway 95, U.S. Highway 1, and railroads, as well as parts of the Capital Beltway and Interstate Highway 66. Extensive Federal land holdings in addition to those in Washington, D.C., include the Marine Corps Development and Education Command at Quantico, Fort Belvoir, Vint Hill Farms Station, the Naval Ordnance Station at Indian Head, the Chesapeake and Ohio Canal National Historic Park, Great Falls Park, and Manassas National Battlefield Park. The quadrangle contains most of Washington, D.C.; part or all of Arlington, Culpeper, Fairfax, Fauquier, Loudoun, Prince William, Rappahannock, and Stafford Counties in northern Virginia; and parts of Charles, Montgomery, and Prince Georges Counties in Maryland.The Washington West quadrangle spans four geologic provinces. From west to east these provinces are the Blue Ridge province, the early Mesozoic Culpeper basin, the Piedmont province, and the Coastal Plain province. There is some overlap in ages of rocks in the Blue Ridge and Piedmont provinces. The Blue Ridge province, which occupies the western part of the quadrangle, contains metamorphic and igneous rocks of Mesoproterozoic to Early Cambrian age. Mesoproterozoic (Grenville-age) rocks are mostly granitic gneisses, although older metaigneous rocks are found as xenoliths. Small areas of Neoproterozoic metasedimentary rocks nonconformably overlie Mesoproterozoic rocks. Neoproterozoic granitic rocks of the Robertson River Igneous Suite intruded

  11. Washington biofuel feedstock crop supply under output price and quantity uncertainty

    International Nuclear Information System (INIS)

    Zheng Qiujie; Shumway, C. Richard

    2012-01-01

    Subsidized development of an in-state biofuels industry has received some political support in the state of Washington, USA. Utilizing in-state feedstock supplies could be an efficient way to stimulate biofuel industries and the local economy. In this paper we estimate supply under output price and quantity uncertainty for major biofuel feedstock crops in Washington. Farmers are expected to be risk averse and maximize the utility of profit and uncertainty. We estimate very large Washington price elasticities for corn and sugar beets but a small price elasticity for a third potential feedstock, canola. Even with the large price elasticities for two potential feedstocks, their current and historical production levels in the state are so low that unrealistically large incentives would likely be needed to obtain sufficient feedstock supply for a Washington biofuel industry. Based on our examination of state and regional data, we find low likelihood that a Washington biofuels industry will develop in the near future primarily using within-state biofuel feedstock crops. - Highlights: ► Within-state feedstock crop supplies insufficient for Washington biofuel industry. ► Potential Washington corn and sugar beet supplies very responsive to price changes. ► Feedstock supplies more responsive to higher expected profit than lower risk. ► R and D for conversion of waste cellulosic feedstocks is potentially important policy.

  12. National Nuclear Waste Policy Act of 1981. Hearings before the Committee on Environment and Public Works, United States Senate, Ninety-Seventh Congress, First Session on S. 1662, October 31, 1981, Richland, Washington; November 9, 1981, Washington, DC

    International Nuclear Information System (INIS)

    Anon.

    1982-01-01

    Hearings were held on October 31, 1981 in Richland, Washington and on November 9, 1981 in Washington, DC to discuss the effort in S. 1662 to establish a national policy and an environmentally acceptable program for managing nuclear wastes from domestic commercial activities. The Richland hearing was held in recognition that Washington State will bear the major impact of the legislation. Witnesses at the Washington, DC hearing included officials from states that are potential sites for radioactive waste storage and disposal facilities. The hearing record includes the testimony of 16 witnesses in Richland and seven in Washington, DC, followed by a reprint of S. 1662 and additional material submitted for the record

  13. Walter Reed Army Medical Center, Washington, D. C. - Annual Progress Report FY-89. Volume 1

    Science.gov (United States)

    1990-01-02

    Enemas Cause Hyperphosphatemia and Acidemia in Patients with Ulcerative Colitis After Routine5 Colonic Cleansing." 4. LCDR Michael S. Opsahl, MC...Cholestyramine Treatment of 101 Thyrotoxicosis (3/88) 1303-88 Burman, Kenneth COL MC. The Clinical Application of In Situ 102 Hybridization to Detect Viral...Absorption of Commercial 144 (Oral) Calcium Carbonate Supplements (10/87) 1398-87 Solomon, Barbara DNSc. The Management of Hyperthyroidism Due 145 to

  14. An Analysis of Wintertime Winds in Washington, D.C.

    Energy Technology Data Exchange (ETDEWEB)

    Berg, Larry K.; Allwine, K Jerry

    2006-06-20

    This report consists of a description of the wintertime climatology of wind speed and wind direction around the National Mall in Washington, D.C. Meteorological data for this study were collected at Ronald Reagan Washington National Airport (Reagan National), Dulles International Airport (Dulles), and a set of surface meteorological stations that are located on a number of building tops around the National Mall. A five-year wintertime climatology of wind speed and wind direction measured at Reagan National and Dulles are presented. A more detailed analysis was completed for the period December 2003 through February 2004 using data gathered from stations located around the National Mall, Reagan National, and Dulles. Key findings of our study include the following: * There are systematic differences between the wind speed and wind direction observed at Reagan National and the wind speed and wind direction measured by building top weather stations located in the National Mall. Although Dulles is located much further from the National Mall than Reagan National, there is better agreement between the wind speed and wind direction measured at Dulles and the weather stations in the National Mall. * When the winds are light (less than 3 ms-1 or 7 mph), there are significant differences in the wind directions reported at the various weather stations within the Mall. * Although the mean characteristics of the wind are similar at the various locations, significant, short-term differences are found when the time series are compared. These differences have important implications for the dispersion of airborne contaminants. In support of wintertime special events in the area of the National Mall, we recommend placing four additional meteorological instruments: three additional surface stations, one on the east bank of the Potomac River, one south of the Reflecting Pool (to better define the flow within the Mall), and a surface station near the Herbert C. Hoover Building; and wind

  15. Population dynamics of mallards breeding in eastern Washington

    Science.gov (United States)

    Dugger, Bruce D.; Coluccy, John M.; Dugger, Katie M.; Fox, Trevor T.; Kraege, Donald K.; Petrie, Mark J.

    2016-01-01

    Variation in regional population trends for mallards breeding in the western United States indicates that additional research into factors that influence demographics could contribute to management and understanding the population demographics of mallards across North America. We estimated breeding incidence and adult female, nest, and brood survival in eastern Washington in 2006 and 2007 by monitoring female mallards with radio telemetry and tested how those parameters were influenced by study year (2006 vs. 2007), landscape type (agricultural vs. natural), and age (second year [SY] vs. after second year [ASY]). We also investigated the effects of female body condition and capture date on breeding incidence, and nest initiation date and hatch date on nest and brood survival, respectively. We included population parameters in a stage-based demographic model and conducted a perturbation analysis to identify which vital rates were most influential on population growth rate (λ). Adult female survival was best modeled with a constant weekly survival rate (0.994, SE = 0.003). Breeding incidence differed between years and was higher for birds in better body condition. Nest survival was higher for ASY females (0.276, SE = 0.118) than SY females (0.066, SE = 0.052), and higher on publicly managed lands (0.383, SE = 0.212) than agricultural (0.114, SE = 0.058) landscapes. Brood survival was best modeled with a constant rate for the 7-week monitoring period (0.50, SE = 0.155). The single variable having the greatest influence on λ was non-breeding season survival, but the combination of parameters from the breeding grounds explained a greater percent of the variance in λ. Mallard population growth rate was most sensitive to changes in non-breeding survival, nest success, brood survival, and breeding incidence. Future management decisions should focus on activities that improve these vital rates if managers want to increase the production of

  16. Geologic map of the Yacolt quadrangle, Clark County, Washington

    Science.gov (United States)

    Evarts, R.C.

    2006-01-01

    The Yacolt 7.5' quadrangle is situated in the foothills of the western Cascade Range of southwestern Washington approximately 35 km northeast of Portland, Oregon. Since late Eocene time, the Cascade Range has been the locus of an active volcanic arc associated with underthrusting of oceanic lithosphere beneath the North American continent along the Cascadia Subduction Zone. Volcanic and shallow-level intrusive rocks emplaced early in the history of the arc underlie most of the Yacolt quadrangle, forming a dissected and partly glaciated terrain with elevations between 250 and 2180 ft (75 and 665 m). The bedrock surface slopes irregularly but steeply to the southwest, forming the eastern margin of the Portland Basin, and weakly consolidated Miocene and younger basin-fill sediments lap up against the bedrock terrain in the southern part of the map area. A deep canyon, carved by the East Fork Lewis River that flows westward out of the Cascade Range, separates Yacolt and Bells Mountains, the two highest points in the quadrangle. Just west of the quadrangle, the river departs from its narrow bedrock channel and enters a wide alluvial floodplain. Bedrock of the Yacolt quadrangle consists of near-horizontal strata of Oligocene volcanic and volcaniclastic rocks that comprise early products of the Cascade volcanic arc. Basalt and basaltic andesite flows predominate. Most were emplaced on the flanks of a large mafic shield volcano and are interfingered with crudely bedded sections of volcanic breccia of probable lahar origin and a variety of well bedded epiclastic sedimentary rocks. At Yacolt Mountain, the volcanogenic rocks are intruded by a body of Miocene quartz diorite that is compositionally distinct from any volcanic rocks in the map area. The town of Yacolt sits in a north-northwest-trending valley apparently formed within a major fault zone. Several times during the Pleistocene, mountain glaciers moved down the Lewis River valley and spread southward into the map area

  17. Termorregulação em colônias de Melipona eburnea (Apidae: Meliponina criadas racionalmente em Rio Branco, Acre

    Directory of Open Access Journals (Sweden)

    Francisco Cildomar da Silva Correia

    2017-08-01

    Full Text Available Resumo. A temperatura é o agente climático que afeta mais diretamente o metabolismo das abelhas, influenciando no desenvolvimento das crias, podendo acarretar resultados negativos para produção de mel. O objetivo deste estudo foi conhecer a diferença da termorregulação de colônias de Melipona eburnea Friese. As observações foram realizadas en­tre 09 de junho e 09 de julho de 2016, 24 horas por dia, utilizando-se dois ninhos de M. eburnea, um em caixa racional e outro em troncode árvore. Utilizou-se Data Logger(modelo HOBO U12 – 012, com exatidão de ±0,35 °C. Verificou-se que o ninho de M. eburnea em oco de árvore manteve uma temperatura média de 31,7 ºC, enquanto que o ninho em caixa racional apresentou temperatura média de 27,8 ºC. A temperatura ambiente variou de 16,6 a 34,2 °C, com média de 23,8 ºC. A espécie M. eburnea em caixa racional apresentou termorregulação inferior à colônia em oco de árvore e também maior oscilação de temperatura. Concluiu-se que M. eburnea mantém sua colônia em homeostase, mesmo quando há variações na temperatura ambiente (ninho em oco de árvore. M. eburnea apresentou termorregulação insatisfatória, quando mantida em caixa racional. Thermoregulation in colonies of Melipona eburnea Friese(Apidae: Meliponina rationally bred in Rio Branco, Acre Abstract. Temperature is the climatic agent that most directly affects the metabolism of bees, influencing at the development of the young being able lead to negative results for honey production. The objective of this study was to know the difference of thermoregulation of Melipona eburnea Friese colonies. The observations were made between June 9 and July 9, 2016, 24 hours a day, using two nests of M. eburnea, one in rational box and the other in tree trunk. Was used Data Logger (model HOBO U12-012 was used, with accuracy of ± 0.35 °C. Was verified that the nest of M. eburnea in tree hollow maintained an average temperature of 31

  18. Improving the Terrain-Based Parameter for the Assessment of Snow Redistribution in the Col du Lac Blanc Area and Comparisons with TLS Snow Depth Data

    Science.gov (United States)

    Schön, Peter; Prokop, Alexander; Naaim-Bouvet, Florence; Nishimura, Kouichi; Vionnet, Vincent; Guyomarc'h, Gilbert

    2014-05-01

    Wind and the associated snow drift are dominating factors determining the snow distribution and accumulation in alpine areas, resulting in a high spatial variability of snow depth that is difficult to evaluate and quantify. The terrain-based parameter Sx characterizes the degree of shelter or exposure of a grid point provided by the upwind terrain, without the computational complexity of numerical wind field models. The parameter has shown to qualitatively predict snow redistribution with good reproduction of spatial patterns, but has failed to quantitatively describe the snow redistribution, and correlations with measured snow heights were poor. The objective of our research was to a) identify the sources of poor correlations between predicted and measured snow re-distribution and b) improve the parameters ability to qualitatively and quantitatively describe snow redistribution in our research area, the Col du Lac Blanc in the French Alps. The area is at an elevation of 2700 m and particularly suited for our study due to its constant wind direction and the availability of data from a meteorological station. Our work focused on areas with terrain edges of approximately 10 m height, and we worked with 1-2 m resolution digital terrain and snow surface data. We first compared the results of the terrain-based parameter calculations to measured snow-depths, obtained by high-accuracy terrestrial laser scan measurements. The results were similar to previous studies: The parameter was able to reproduce observed patterns in snow distribution, but regression analyses showed poor correlations between terrain-based parameter and measured snow-depths. We demonstrate how the correlations between measured and calculated snow heights improve if the parameter is calculated based on a snow surface model instead of a digital terrain model. We show how changing the parameter's search distance and how raster re-sampling and raster smoothing improve the results. To improve the parameter

  19. Demise of the northern Tethyan Urgonian carbonate platform and subsequent transition towards pelagic conditions: The sedimentary record of the Col de la Plaine Morte area, central Switzerland

    Science.gov (United States)

    Föllmi, Karl B.; Gainon, François

    2008-04-01

    The sedimentary succession of the Col de la Plaine Morte area (Helvetic Alps, central Switzerland) documents the disappearance of the northern Tethyan Urgonian platform in unprecedented detail and suggests stepwise platform demise, with each drowning phase documented by erosion and phosphogenesis. The first identified drowning phase terminated Urgonian carbonate production in a predominantly photozoan mode. Using a correlation of the whole-rock δ13C record with the well-dated record from SE France, its age is inferred to as Middle Early Aptian (near the boundary between the weissi and deshayesi zones). A subsequent drowning phase is dated by ammonites and by a correlation of the whole-rock δ13C record as Late Early Aptian (late deshayesi to early furcata zone). A third drowning phase provides an ammonite-based age of Early Late Aptian ( subnodosocostatum and melchioris zones) and is part of a widely recognized phase of sediment condensation and phosphogenesis, which is dated as latest Early to Middle Late Aptian (late furcata zone to near the boundary of the melchioris and nolani zones). The fourth and final drowning phase started in the latest Aptian ( jacobi zone) as is also indicated by ammonite findings at the Col de la Plaine Morte. The phases of renewed platform-carbonate production intervening between the drowning phases were all in a heterozoan mode. During the ultimate drowning phase, phosphogenesis continued until the Early Middle Albian, whereas condensation processes lasted until the Middle Turonian. Coverage of the external margin of the drowned Urgonian platform by a drape of pelagic carbonates started only in the Late Turonian. During the Santonian, the external part of the drowned platform underwent normal faulting and saw the re-exposure of already lithified Urgonian carbonates at the seafloor. Based on the here-inferred ages, the first drowning phase just precedes oceanic anoxic episode 1a (OAE 1a or "selli event") in time, and the second

  20. COL5A1 gene variants previously associated with reduced soft tissue injury risk are associated with elite athlete status in rugby.

    Science.gov (United States)

    Heffernan, Shane M; Kilduff, Liam P; Erskine, Robert M; Day, Stephen H; Stebbings, Georgina K; Cook, Christian J; Raleigh, Stuart M; Bennett, Mark A; Wang, Guan; Collins, Malcolm; Pitsiladis, Yannis P; Williams, Alun G

    2017-11-14

    Two common single nucleotide polymorphisms within the COL5A1 gene (SNPs; rs12722 C/T and rs3196378 C/A) have previously been associated with tendon and ligament pathologies. Given the high incidence of tendon and ligament injuries in elite rugby athletes, we hypothesised that both SNPs would be associated with career success. In 1105 participants (RugbyGene project), comprising 460 elite rugby union (RU), 88 elite rugby league athletes and 565 non-athlete controls, DNA was collected and genotyped for the COL5A1 rs12722 and rs3196378 variants using real-time PCR. For rs12722, the injury-protective CC genotype and C allele were more common in all athletes (21% and 47%, respectively) and RU athletes (22% and 48%) than in controls (16% and 41%, P ≤ 0.01). For rs3196378, the CC genotype and C allele were overrepresented in all athletes (23% and 48%) and RU athletes (24% and 49%) compared with controls (16% and 41%, P ≤ 0.02). The CC genotype in particular was overrepresented in the back and centres (24%) compared with controls, with more than twice the odds (OR = 2.25, P = 0.006) of possessing the injury-protective CC genotype. Furthermore, when considering both SNPs simultaneously, the CC-CC SNP-SNP combination and C-C inferred allele combination were higher in all the athlete groups (≥18% and ≥43%) compared with controls (13% and 40%; P = 0.01). However, no genotype differences were identified for either SNP when RU playing positions were compared directly with each other. It appears that the C alleles, CC genotypes and resulting combinations of both rs12722 and rs3196378 are beneficial for rugby athletes to achieve elite status and carriage of these variants may impart an inherited resistance against soft tissue injury, despite exposure to the high-risk environment of elite rugby. These data have implications for the management of inter-individual differences in injury risk amongst elite athletes.

  1. Three new genetic loci (R1210C in CFH, variants in COL8A1 and RAD51B are independently related to progression to advanced macular degeneration.

    Directory of Open Access Journals (Sweden)

    Johanna M Seddon

    Full Text Available To assess the independent impact of new genetic variants on conversion to advanced stages of AMD, controlling for established risk factors, and to determine the contribution of genes in predictive models.In this prospective longitudinal study of 2765 individuals, 777 subjects progressed to neovascular disease (NV or geographic atrophy (GA in either eye over 12 years. Recently reported genetic loci were assessed for their independent effects on incident advanced AMD after controlling for 6 established loci in 5 genes, and demographic, behavioral, and macular characteristics. New variants which remained significantly related to progression were then added to a final multivariate model to assess their independent effects. The contribution of genes to risk models was assessed using reclassification tables by determining risk within cross-classified quintiles for alternative models.THREE NEW GENETIC VARIANTS WERE SIGNIFICANTLY RELATED TO PROGRESSION: rare variant R1210C in CFH (hazard ratio (HR 2.5, 95% confidence interval [CI] 1.2-5.3, P = 0.01, and common variants in genes COL8A1 (HR 2.0, 95% CI 1.1-3.5, P = 0.02 and RAD51B (HR 0.8, 95% CI 0.60-0.97, P = 0.03. The area under the curve statistic (AUC was significantly higher for the 9 gene model (.884 vs the 0 gene model (.873, P = .01. AUC's for the 9 vs 6 gene models were not significantly different, but reclassification analyses indicated significant added information for more genes, with adjusted odds ratios (OR for progression within 5 years per one quintile increase in risk score of 2.7, P<0.001 for the 9 vs 6 loci model, and OR 3.5, P<0.001 for the 9 vs. 0 gene model. Similar results were seen for NV and GA.Rare variant CFH R1210C and common variants in COL8A1 and RAD51B plus six genes in previous models contribute additional predictive information for advanced AMD beyond macular and behavioral phenotypes.

  2. Merging a Terrain-Based Parameter and Snow Particle Counter Data for the Assessment of Snow Redistribution in the Col du Lac Blanc Area

    Science.gov (United States)

    Schön, Peter; Prokop, Alexander; Naaim-Bouvet, Florence; Vionnet, Vincent; Guyomarc'h, Gilbert; Heiser, Micha; Nishimura, Kouichi

    2015-04-01

    Wind and the associated snow drift are dominating factors determining the snow distribution and accumulation in alpine areas, resulting in a high spatial variability of snow depth that is difficult to evaluate and quantify. The terrain-based parameter Sx characterizes the degree of shelter or exposure of a grid point provided by the upwind terrain, without the computational complexity of numerical wind field models. The parameter has shown to qualitatively predict snow redistribution with good reproduction of spatial patterns. It does not, however, provide a quantitative estimate of changes in snow depths. The objective of our research was to introduce a new parameter to quantify changes in snow depths in our research area, the Col du Lac Blanc in the French Alps. The area is at an elevation of 2700 m and particularly suited for our study due to its consistently bi-modal wind directions. Our work focused on two pronounced, approximately 10 m high terrain breaks, and we worked with 1 m resolution digital snow surface models (DSM). The DSM and measured changes in snow depths were obtained with high-accuracy terrestrial laser scan (TLS) measurements. First we calculated the terrain-based parameter Sx on a digital snow surface model and correlated Sx with measured changes in snow-depths (Δ SH). Results showed that Δ SH can be approximated by Δ SHestimated = α * Sx, where α is a newly introduced parameter. The parameter α has shown to be linked to the amount of snow deposited influenced by blowing snow flux. At the Col du Lac Blanc test side, blowing snow flux is recorded with snow particle counters (SPC). Snow flux is the number of drifting snow particles per time and area. Hence, the SPC provide data about the duration and intensity of drifting snow events, two important factors not accounted for by the terrain parameter Sx. We analyse how the SPC snow flux data can be used to estimate the magnitude of the new variable parameter α . To simulate the development

  3. RCRA groundwater data analysis protocol for the Hanford Site, Washington

    International Nuclear Information System (INIS)

    Chou, C.J.; Jackson, R.L.

    1992-04-01

    The Resource Conservation and Recovery Act of 1976 (RCRA) groundwater monitoring program currently involves site-specific monitoring of 20 facilities on the Hanford Site in southeastern Washington. The RCRA groundwater monitoring program has collected abundant data on groundwater quality. These data are used to assess the impact of a facility on groundwater quality or whether remediation efforts under RCRA corrective action programs are effective. Both evaluations rely on statistical analysis of groundwater monitoring data. The need for information on groundwater quality by regulators and environmental managers makes statistical analysis of monitoring data an important part of RCRA groundwater monitoring programs. The complexity of groundwater monitoring programs and variabilities (spatial, temporal, and analytical) exhibited in groundwater quality variables indicate the need for a data analysis protocol to guide statistical analysis. A data analysis protocol was developed from the perspective of addressing regulatory requirements, data quality, and management information needs. This data analysis protocol contains four elements: data handling methods; graphical evaluation techniques; statistical tests for trend, central tendency, and excursion analysis; and reporting procedures for presenting results to users

  4. Science meets public service in Washington, D.C.

    Science.gov (United States)

    Vasavada, Ashwin R.

    Same planet, different worlds—that's how many scientists see the relationship between science and government. Yet science and technology have become so infused into society that those worlds are colliding. Today, a number of national issues share a strong connection to science, from stem cells to climate change and energy to bioterrorism. For scientists who can adapt to the culture of politics, working in the collision zone can be an exciting and rewarding way to spend a year or even a career.This past year, I was one of 35 scientists in Washington serving as Congressional Science and Technology Fellows, sponsored by a number of scientific societies, including AGU. The Fellows vary widely in age and carry resumes listing Ph.D.s in not only physics, biology, and chemistry but also in Earth science, food safety, psychology, and veterinary medicine. With a group like that, weekly lunches and happy hours become the kind of broadening experience that one rarely gets in focused academic departments. And then there's the politics.

  5. HIV intertest interval among MSM in King County, Washington.

    Science.gov (United States)

    Katz, David A; Dombrowski, Julia C; Swanson, Fred; Buskin, Susan E; Golden, Matthew R; Stekler, Joanne D

    2013-02-01

    The authors examined temporal trends and correlates of HIV testing frequency among men who have sex with men (MSM) in King County, Washington. The authors evaluated data from MSM testing for HIV at the Public Health-Seattle & King County (PHSKC) STD Clinic and Gay City Health Project (GCHP) and testing history data from MSM in PHSKC HIV surveillance. The intertest interval (ITI) was defined as the number of days between the last negative HIV test and the current testing visit or first positive test. Correlates of the log(10)-transformed ITI were determined using generalised estimating equations linear regression. Between 2003 and 2010, the median ITI among MSM seeking HIV testing at the STD Clinic and GCHP were 215 (IQR: 124-409) and 257 (IQR: 148-503) days, respectively. In multivariate analyses, younger age, having only male partners and reporting ≥10 male sex partners in the last year were associated with shorter ITIs at both testing sites (pGCHP attendees, having a regular healthcare provider, seeking a test as part of a regular schedule and inhaled nitrite use in the last year were also associated with shorter ITIs (pGCHP (median 359 vs 255 days, p=0.02). Although MSM in King County appear to be testing at frequent intervals, further efforts are needed to reduce the time that HIV-infected persons are unaware of their status.

  6. Ephemeral seafloor sedimentation during dam removal: Elwha River, Washington

    Science.gov (United States)

    Foley, Melissa M.; Warrick, Jonathan A.

    2017-11-01

    The removal of the Elwha and Glines Canyon dams from the Elwha River in Washington, USA, resulted in the erosion and transport of over 10 million m3 of sediment from the former reservoirs and into the river during the first two years of the dam removal process. Approximately 90% of this sediment was transported through the Elwha River and to the coast at the Strait of Juan de Fuca. To evaluate the benthic dynamics of increased sediment loading to the nearshore, we deployed a tripod system in ten meters of water to the east of the Elwha River mouth that included a profiling current meter and a camera system. With these data, we were able to document the frequency and duration of sedimentation and turbidity events, and correlate these events to physical oceanographic and river conditions. We found that seafloor sedimentation occurred regularly during the heaviest sediment loading from the river, but that this sedimentation was ephemeral and exhibited regular cycles of deposition and erosion caused by the strong tidal currents in the region. Understanding the frequency and duration of short-term sediment disturbance events is instrumental to interpreting the ecosystem-wide changes that are occurring in the nearshore habitats around the Elwha River delta.

  7. Tectonic setting of the Wooded Island earthquake swarm, eastern Washington

    Science.gov (United States)

    Blakely, Richard J.; Sherrod, Brian L.; Weaver, Craig S.; Rohay, Alan C.; Wells, Ray E.

    2012-01-01

    Magnetic anomalies provide insights into the tectonic implications of a swarm of ~1500 shallow (~1 km deep) earthquakes that occurred in 2009 on the Hanford site,Washington. Epicenters were concentrated in a 2 km2 area nearWooded Island in the Columbia River. The largest earthquake (M 3.0) had first motions consistent with slip on a northwest-striking reverse fault. The swarm was accompanied by 35 mm of vertical surface deformation, seen in satellite interferometry (InSAR), interpreted to be caused by ~50 mm of slip on a northwest-striking reverse fault and associated bedding-plane fault in the underlying Columbia River Basalt Group (CRBG). A magnetic anomaly over exposed CRBG at Yakima Ridge 40 km northwest of Wooded Island extends southeastward beyond the ridge to the Columbia River, suggesting that the Yakima Ridge anticline and its associated thrust fault extend southeastward in the subsurface. In map view, the concealed anticline passes through the earthquake swarm and lies parallel to reverse faults determined from first motions and InSAR data. A forward model of the magnetic anomaly near Wooded Island is consistent with uplift of concealed CRBG, with the top surface swarm and the thrust and bedding-plane faults modeled from interferometry all fall within the northeastern limb of the faulted anticline. Although fluids may be responsible for triggering the Wooded Island earthquake swarm, the seismic and aseismic deformation are consistent with regional-scale tectonic compression across the concealed Yakima Ridge anticline.

  8. University of Washington Lab Report to SNEAP, 1999

    International Nuclear Information System (INIS)

    Amsbaugh, J.F.; Harper, G.C.; Linder, C.E.; Myers, A.W.; Storns, D.W.; Wechel, T.D. van; Will, D.I.

    2000-01-01

    OAK-B135 University of Washington Lab Report to SNEAP, 1999. The NPL tandem Van de Graaff accelerator chains ran 1869 hours between 9/1/98 and 8/31/99. The DEIS ran 356 hours and the SpIS ran 667. There were 28 experiment-days in which the LINAC and tandem were used together and seven days when the tandem was used, with one of the external ion sources, as a tandem. Four of these were with light ions and three with heavy ions. There were 68 days of Tandem Terminal Ion Source (TIS) development or repair, and 46 days of TIS installation or removal or changing TIS ion species. The TIS/tandem was used a total of 70 days to deliver p, d, 3 He, or 4 He beams for use by an experimenter. There were 47 experiment-days during which the injector deck was used to generate C 60 or LiF 3 clusters, which were then investigated without further acceleration. They had 25 tank openings over the year. There were three scheduled openings for the installation or removal of the TIS, and seven to change the TIS ion species. There were 10 openings for 4 He ion source and column resistor gradient development. There were four openings for various repairs other than ion source, and one to replace the LE grid, which was found undamaged after all

  9. Asotin Creek Model Watershed Plan: Asotin County, Washington, 1995.

    Energy Technology Data Exchange (ETDEWEB)

    Browne, Dave

    1995-04-01

    The Northwest Power Planning Council completed its ``Strategy for Salmon'' in 1992. This is a plan, composed of four specific elements,designed to double the present production of 2.5 million salmon in the Columbia River watershed. These elements have been called the ``four H's'': (1) improve harvest management; (2) improve hatcheries and their production practices; (3) improve survival at hydroelectric dams; and (4) improve and protect fish habitat. The Asotin Creek Model Watershed Plan is the first to be developed in Washington State which is specifically concerned with habitat protection and restoration for salmon and trout. The plan is consistent with the habitat element of the ``Strategy for Salmon''. Asotin Creek is similar in many ways to other salmon-bearing streams in the Snake River system. Its watershed has been significantly impacted by human activities and catastrophic natural events, such as floods and droughts. It supports only remnant salmon and trout populations compared to earlier years. It will require protection and restoration of its fish habitat and riparian corridor in order to increase its salmonid productivity.

  10. Ephemeral seafloor sedimentation during dam removal: Elwha River, Washington

    Science.gov (United States)

    Foley, Melissa M.; Warrick, Jonathan

    2017-01-01

    The removal of the Elwha and Glines Canyon dams from the Elwha River in Washington, USA, resulted in the erosion and transport of over 10 million m3 of sediment from the former reservoirs and into the river during the first two years of the dam removal process. Approximately 90% of this sediment was transported through the Elwha River and to the coast at the Strait of Juan de Fuca. To evaluate the benthic dynamics of increased sediment loading to the nearshore, we deployed a tripod system in ten meters of water to the east of the Elwha River mouth that included a profiling current meter and a camera system. With these data, we were able to document the frequency and duration of sedimentation and turbidity events, and correlate these events to physical oceanographic and river conditions. We found that seafloor sedimentation occurred regularly during the heaviest sediment loading from the river, but that this sedimentation was ephemeral and exhibited regular cycles of deposition and erosion caused by the strong tidal currents in the region. Understanding the frequency and duration of short-term sediment disturbance events is instrumental to interpreting the ecosystem-wide changes that are occurring in the nearshore habitats around the Elwha River delta.

  11. Drilling history core hole DC-6 Hanford, Washington

    International Nuclear Information System (INIS)

    1978-06-01

    Core hole DC-6 was completed in May 1978 by Boyles Brothers Drilling Company, Spokane, Washington, under subcontract to Fenix and Scisson, Inc. The hole was cored for the US Department of Energy and the Rockwell Hanford Operations' Basalt Waste Isolation Program. Fenix and Scisson, Inc. furnished the engineering, daily supervision of the core drilling activities, and geologic core logging for hole DC-6. Core hole DC-6 is located within the boundary of the Hanford Site at the old Hanford town site. The Hanford Site coordinates for DC-6 are North 54,127.17 feet and West 17,721.00 feet. The surface elevation is approximately 402 feet above sea level. The purpose of core hole DC-6 was to core drill vertically through the basalt and interbed units for stratigraphic depth determination and core collection and to provide a borehole for hydrologic testing. The total depth of core hole DC-6 was 4336 feet. Core recovery was 98.4% of the total footage cored

  12. Geothermal : Economic Impacts of Geothermal Development in Whatcom County, Washington.

    Energy Technology Data Exchange (ETDEWEB)

    Lesser, Jonathan A.

    1992-07-01

    This report estimates the local economic impacts that could be anticipated from the development of a 100 megawatt (MW) geothermal power plant in eastern Whatcom County, Washington, near Mt. Baker, as shown in Figure 1. The study was commissioned by the Bonneville Power Administration to quantify such impacts as part of regional confirmation work recommended by the Northwest Power Planning Council. Whatcom County was chosen due to both identified geotherrnal resources and developer interest. The analysis will focus on two phases: a plant construction phase, including well field development, generating plant construction, and transmission line construction; and an operations phase. Economic impacts will occur to the extent that construction and operations affect the local economy. These impacts will depend on the existing structure of the Whatcom County economy and estimates of revenues that may accrue to the county as a result of plant construction, operation, and maintenance. Specific impacts may include additional direct employment at the plant, secondary impacts from wage payments being used to purchase locally produced goods and services, and impacts due to expenditures of royalty and tax payments received by the county. The basis for the analysis of economic impacts in this study is the US Forest Service IMPLAN input-output modeling system.

  13. Biomarkers of Sensitivity and Exposure in Washington State Pesticide Handlers

    Science.gov (United States)

    Keifer, M.C.; Checkoway, H.; De Roos, A.J.; Farin, F.M.; Fenske, R.A.; Richter, R.J.; van Belle, G.; Furlong, C.E.

    2011-01-01

    Organophosphate (OP) and N-methyl-carbamate (CB) insecticides are widely used in agriculture in the US and abroad. These compounds – which inhibit acetylcholinesterase (AChE) enzyme activity – continue to be responsible for a high proportion of pesticide poisonings among US agricultural workers. It is possible that some individuals may be especially susceptible to health effects related to OP/CB exposure. The paraoxonase (PON1) enzyme metabolizes the highly toxic oxon forms of some OPs, and an individual's PON1 status may be an important determinant of his or her sensitivity to these chemicals. This chapter discusses methods used to characterize individual PON1 status and reviews previous epidemiologic studies that have evaluated PON1-related sensitivity to OPs in relation to various health endpoints. It also describes an ongoing longitudinal study among OP-exposed agricultural pesticide handlers who are participating in a recently implemented cholinesterase monitoring program in Washington State. This study will evaluate handlers' PON1 status as a hypothesized determinant of butyrylcholinesterase (BuChE) inhibition. Such studies will be useful to determine how regulatory risk assessments might account for differences in PON1-related OP sensitivity when characterizing inter-individual variability in risk related to OP exposure. Recent work assessing newer and more sensitive biomarkers of OP exposure is also discussed briefly in this chapter. PMID:20221867

  14. Environmental Assessment: Waste Tank Safety Program, Hanford Site, Richland, Washington

    International Nuclear Information System (INIS)

    1994-02-01

    The US Department of Energy (DOE) needs to take action in the near-term, to accelerate resolution of waste tank safety issues at the Hanford Site near the City of Richland, Washington, and reduce the risks associated with operations and management of the waste tanks. The DOE has conducted nuclear waste management operations at the Hanford Site for nearly 50 years. Operations have included storage of high-level nuclear waste in 177 underground storage tanks (UST), both in single-shell tank (SST) and double-shell tank configurations. Many of the tanks, and the equipment needed to operate them, are deteriorated. Sixty-seven SSTs are presumed to have leaked a total approximately 3,800,000 liters (1 million gallons) of radioactive waste to the soil. Safety issues associated with the waste have been identified, and include (1) flammable gas generation and episodic release; (2) ferrocyanide-containing wastes; (3) a floating organic solvent layer in Tank 241-C-103; (4) nuclear criticality; (5) toxic vapors; (6) infrastructure upgrades; and (7) interim stabilization of SSTs. Initial actions have been taken in all of these areas; however, much work remains before a full understanding of the tank waste behavior is achieved. The DOE needs to accelerate the resolution of tank safety concerns to reduce the risk of an unanticipated radioactive or chemical release to the environment, while continuing to manage the wastes safely

  15. The 22 March 2014 Oso landslide, Washington, USA

    Science.gov (United States)

    Wartman, Joseph; Montgomery, David R.; Anderson, Scott A.; Keaton, Jeffrey R.; Benoît, Jean; dela Chapelle, John; Gilbert, Robert

    2016-01-01

    The Oso, Washington, USA, landslide occurred on the morning of Saturday, 22 March 2014 and claimed the lives of 43 people. The landslide began within an 200-m-high hillslope comprised of unconsolidated glacial and previous landslide/colluvial deposits; it continued as a debris avalanche/debris flow that rapidly inundated a neighborhood of 35 single-family residences. An intense three-week rainfall that immediately preceded the event most likely played a role in triggering the landslide; and other factors that likely contributed to destabilization of the landslide mass include alteration of the local groundwater recharge and hydrogeological regime from previous landsliding, weakening and alteration of the landslide mass caused by previous landsliding, and changes in stress distribution resulting from removal and deposition of material from earlier landsliding. Field reconnaissance following the event revealed six distinctive zones and several subzones that are characterized on the basis of geomorphic expression, styles of deformation, geologic materials, and the types, size, and orientation of vegetation. Seismic recording of the landslide indicate that the event was marked by several vibration-generating episodes of mass movement. We hypothesize that the landslide occurred in two stages, with the first being a sequential remobilization of existing slide masses from the most recent (2006) landslide and from an ancient slide that triggered a devastating debris avalanche/debris flow. The second stage involved headward extension into previously unfailed material that occurred in response to unloading and redirection of stresses.

  16. Environmental monitoring at Hanford by the state of Washington

    International Nuclear Information System (INIS)

    Conklin, A.W.; Mooney, R.R.; Erickson, J.L.

    1990-01-01

    The Department of Social and Health Services' Office of Radiation Protection (ORP), Washington State's radiation control agency, has a mandate to protect the public from radiation. In 1985, ORP was instructed by the legislature to establish a statewide environmental radiological base line, beginning with Hanford, to verify federal environmental programs, and to enforce federal and state Clean Air Acts. The primary mission of the agency is to protect public health by active involvement in Hanford monitoring and oversight. The state's program was designed not to duplicate but to supplement existing programs and to identify any sampling gaps or problems. Split, side-by-side, and independent samples are collected, with analysis performed by the state's own laboratory. Media sampled have included surface and drinking water, seep and ground water, fruits and vegetables, milk, soils, and air particulates; ambient radiation levels have been determined. Special activities have included split sampling of river seeps with multiple agencies, preliminary dose assessment of early Hanford releases, investigations of 129 I in the environment and in Franklin County drinking water, verification of U.S. Department of Energy (DOE) data on erroneous alarms at the Hanford Plutonium Uranium Extraction Plant, split sampling with a DOE headquarters survey, and participation in several General Accounting Office investigations and a National Academy of Sciences review. The independence of ORP programs guarantees that the public has access to environmental data on the activities of DOE and its contractors. We will describe the interrelationship of ORP and Hanford programs and present results of ORP activities

  17. Preliminary geology of eastern Umtanum Ridge, South-Central Washington

    Energy Technology Data Exchange (ETDEWEB)

    Goff, F.E.

    1981-01-01

    The basalt stratigraphy and geologic structures of eastern Umtanum Ridge have been mapped and studied in detail to help assess the feasibility of nuclear waste terminal storage on the Hanford Site in southeastern Washington State. Eastern Umtanum Ridge is an asymmetric east-west-trending anticline of Columbia River basalt that plunges 5 degrees eastward into the Pasco Basin. Geologic mapping and determination of natural remanent magnetic polarity and chemical composition reveal that flows of the Pomona and Umatilla Members (Saddle Mountains Basalt), Priest Rapids and Frenchman Springs Members (Wanapum Basalt), and Grande Ronde Basalt were erupted as fairly uniform sheets. The Wahluke and Huntzinger flows (Saddle Mountains Basalt) fill a paleovalley cut into Wanapum Basalt. No evidence was found to indicate Quaternary-age movement on any structures in the map area. The basalt strata on the south limb of the Umtanum anticline display relatively little tectonic deformation since Miocene-Pliocene time. Thus, the buried south flank of Umtanum Ridge may provide an excellent location for a nuclear waste repository beneath the Hanford Site.

  18. Has spring snowpack declined in the Washington Cascades?

    Directory of Open Access Journals (Sweden)

    P. Mote

    2008-02-01

    Full Text Available Our best estimates of 1 April snow water equivalent (SWE in the Cascade Mountains of Washington State indicate a substantial (roughly 15–35% decline from mid-century to 2006, with larger declines at low elevations and smaller declines or increases at high elevations. This range of values includes estimates from observations and hydrologic modeling, reflects a range of starting points between about 1930 and 1970 and also reflects uncertainties about sampling. The most important sampling issue springs from the fact that half the 1 April SWE in the Cascades is found below about 1240 m, altitudes at which sampling was poor before 1945. Separating the influences of temperature and precipitation on 1 April SWE in several ways, it is clear that long-term trends are dominated by trends in temperature, whereas variability in precipitation adds "noise" to the time series. Consideration of spatial and temporal patterns of change rules out natural variations like the Pacific Decadal Oscillation as the sole cause of the decline. Regional warming has clearly played a role, but it is not yet possible to quantify how much of that regional warming is related to greenhouse gas emissions.

  19. Quality of ground water in Clark County, Washington, 1988

    International Nuclear Information System (INIS)

    Turney, G.L.

    1990-01-01

    Water samples were collected from 76 wells throughout Clark County, in southwest Washington, during April and May 1988, and were analyzed from concentrations of major ions, silica, nitrate, phosphorus, aluminum, manganese, radon, and bacteria. Samples from 20 wells were analyzed for concentrations of trace elements and organic compounds, including most of those on the US Environmental Protection Agency (USEPA) priority pollutant list. Dissolved solids concentrations range from 12 to 245 mg/L, with a median concentration of 132 mg/L. The major dissolved constituents are calcium, bicarbonate, and silica, and, in some samples, sodium. Nitrate concentrations exceeded 1.0 mg/L throughout the Vancouver urban area, and were as large as 6.7 mg/L. Comparison with limited historical data indicates that nitrate concentrations were somewhat correlated, possibly indicating similar sources. Volatile organic compound, including tetrachloroethane and 1,1,1-trichloroethane, were detected in samples from three wells in the Vancouver area. Trace amounts of volatile organic compounds were reported in samples from several other wells, but at concentrations too close to analytical detection limits to ascertain that they were in the groundwater. Trace elements and radiochemical constituents were present at small levels indicating natural sources for these constituents. Only pH, turbidity, iron, manganese, and total coliform bacteria had values that did not meet USEPA Drinking Water Standards

  20. Economic impacts of geothermal development in Skamania County, Washington

    International Nuclear Information System (INIS)

    Lesser, J.A.

    1992-07-01

    This report estimates the local economic impacts that could be anticipated from the development of a 100 megawatt (MW) geothermal power plant in eastern Skamania County, Washington, near Mt. Adams, as shown in Figure 1. The study was commissioned by the Bonneville Power Administration to quantify such impacts as part of regional confirmation work recommended by the Northwest Power Planning Council. Skamania County was chosen due to both identified geothermal resources and developer interest. The analysis will focus on two phases: a plant construction phase, including well field development, generating plant construction, and transmission line construction; and an operations phase. Economic impacts will occur to the extent that construction and operations affect the local economy. These impacts will depend on the existing structure of the Skamania County economy and estimates of revenues that may accrue to the county as a result of plant construction, operation, and maintenance. Specific impacts may include additional direct employment at the plant, secondary impacts from wage payments being used to purchase locally produced goods and services, and impacts due to expenditures of royalty and tax payments received by the county. The basis for the analysis of economic impacts in this study is the US Forest Service IMPLAN input-output modeling system

  1. Economic impacts of geothermal development in Whatcom County, Washington

    International Nuclear Information System (INIS)

    Lesser, J.A.

    1992-07-01

    This report estimates the local economic impacts that could be anticipated from the development of a 100 megawatt (MW) geothermal power plant in eastern Whatcom County, Washington, near Mt. Baker, as shown in Figure 1. The study was commissioned by the Bonneville Power Administration to quantify such impacts as part of regional confirmation work recommended by the Northwest Power Planning Council. Whatcom County was chosen due to both identified geotherrnal resources and developer interest. The analysis will focus on two phases: a plant construction phase, including well field development, generating plant construction, and transmission line construction; and an operations phase. Economic impacts will occur to the extent that construction and operations affect the local economy. These impacts will depend on the existing structure of the Whatcom County economy and estimates of revenues that may accrue to the county as a result of plant construction, operation, and maintenance. Specific impacts may include additional direct employment at the plant, secondary impacts from wage payments being used to purchase locally produced goods and services, and impacts due to expenditures of royalty and tax payments received by the county. The basis for the analysis of economic impacts in this study is the US Forest Service IMPLAN input-output modeling system

  2. Cyanotoxin bioaccumulation in freshwater fish, Washington State, USA.

    Science.gov (United States)

    Hardy, F Joan; Johnson, Art; Hamel, Kathy; Preece, Ellen

    2015-11-01

    Until recently, exposure pathways of concern for cyanotoxins have focused on recreational exposure, drinking water, and dermal contact. Exposure to cyanotoxins through fish consumption is a relatively new area of investigation. To address this concern, microcystins and other cyanotoxins were analyzed in fish collected from nine Washington lakes with recurrent toxic blooms using two types of enzyme-linked immunosorbent assays (ELISAs) and liquid chromatography/mass spectrometry/mass spectrometry (LC-MS/MS). Microcystins or microcystin-like compounds were elevated in fish liver relative to muscle and other tissues (liver>gut>muscle). Microcystin concentrations in fish fillet samples using anti-Adda ELISA (range 6.3-11 μg/kg wet weight) were consistently higher in all fish species than concentrations using anti-microcystin (MC)-leucine-arginine (LR) ELISA (range 0.25-2.4 μg/kg wet weight). MC-leucine-alanine (LA) was the only variant detected in fish (2.5-12 μg/kg in four livers) among the nine variants analyzed by LC-MS/MS. Fish fillets showed no accumulation of the MCs targeted by LC-MS/MS. Other cyanotoxins analyzed (anatoxin-a, saxitoxin, domoic acid, and okadaic acid) were not detected in fish. Based on this and evidence from other studies, we believe that people can safely consume two 8-oz fish fillet meals per week from lakes with blooms producing MCs (clean the fish and discard viscera).

  3. Asotin Creek model watershed plan: Asotin County, Washington

    International Nuclear Information System (INIS)

    1995-01-01

    The Northwest Power Planning Council completed its ''Strategy for Salmon'' in 1992. This is a plan, composed of four specific elements,designed to double the present production of 2.5 million salmon in the Columbia River watershed. These elements have been called the ''four H's'': (1) improve harvest management; (2) improve hatcheries and their production practices; (3) improve survival at hydroelectric dams; and (4) improve and protect fish habitat. The Asotin Creek Model Watershed Plan is the first to be developed in Washington State which is specifically concerned with habitat protection and restoration for salmon and trout. The plan is consistent with the habitat element of the ''Strategy for Salmon''. Asotin Creek is similar in many ways to other salmon-bearing streams in the Snake River system. Its watershed has been significantly impacted by human activities and catastrophic natural events, such as floods and droughts. It supports only remnant salmon and trout populations compared to earlier years. It will require protection and restoration of its fish habitat and riparian corridor in order to increase its salmonid productivity

  4. Cardiopulmonary resuscitation training in Washington state public high schools.

    Science.gov (United States)

    Reder, Sheri; Quan, Linda

    2003-03-01

    To determine the best approaches for increasing cardiopulmonary resuscitation (CPR) training opportunities for public high school students, we conducted a statewide survey of all 310 public high schools in Washington State. The findings describe CPR student training currently provided by high schools, barriers to providing, and strategies to increase CPR training of high school students. The response rate was 89% (276 schools) from a combination of mail and telephone surveys; 35% (n=97) reported that they did not provide any CPR student training. Of the 132 schools that provided CPR student training, 23% trained less than 10% of their students, and 39% trained more than 90% of their students. The majority of public high schools, 70%, did not have any teacher trained to teach CPR or had only one teacher with such training. Yet 80% of schools felt that CPR training is best provided in school settings. Schools perceived the greatest benefit of CPR training as providing students with the skill to save a life (43%). The most frequently identified barriers were logistical: limited time to teach the curriculum (24%), lack of funds (16%), and instructor scheduling difficulties (17%). Less than 5% of respondents voiced any opposition to CPR training, and that opposition was for logistical reasons. To increase CPR training, the single best strategies suggested were: increase funding, provide time in the curriculum, have more certified instructors, and make CPR student training a requirement.

  5. Seismic hazard assessment of the Hanford region, Eastern Washington State

    International Nuclear Information System (INIS)

    Youngs, R.R.; Coppersmith, K.J.; Power, M.S.; Swan, F.H. III

    1985-01-01

    A probabilistic seismic hazard assessment was made for a site within the Hanford region of eastern Washington state, which is characterized as an intraplate region having a relatively low rate of seismic activity. Probabilistic procedures, such as logic trees, were utilized to account for the uncertainties in identifying and characterizing the potential seismic sources in the region. Logic trees provide a convenient, flexible means of assessing the values and relative likelihoods of input parameters to the hazard model that may be dependent upon each other. Uncertainties accounted for in this way include the tectonic model, segmentation, capability, fault geometry, maximum earthquake magnitude, and earthquake recurrence rate. The computed hazard results are expressed as a distribution from which confidence levels are assessed. Analysis of the results show the contributions to the total hazard from various seismic sources and due to various earthquake magnitudes. In addition, the contributions of uncertainties in the various source parameters to the uncertainty in the computed hazard are assessed. For this study, the major contribution to uncertainty in the computed hazard are due to uncertainties in the applicable tectonic model and the earthquake recurrence rate. This analysis serves to illustrate some of the probabilistic tools that are available for conducting seismic hazard assessments and for analyzing the results of these studies. 5 references, 7 figures

  6. Monitoring fish, wildlife, radionuclides and chemicals at Hanford, Washington

    International Nuclear Information System (INIS)

    Gray, R.H.

    1989-02-01

    Concern about the effects of potential releases from nuclear and non-nuclear activities on the US Department of Energy's Hanford Site in southeastern Washington has evolved over four decades into a comprehensive environmental monitoring and surveillance program. The program includes field sampling, and chemical and physical analyses of air, surface and ground water, fish, wildlife, soil, foodstuffs, and natural vegetation. In addition to monitoring radioactivity in fish and wildlife, population numbers of key species are determined, usually during the breeding season. Data from monitoring efforts are used to assess the environmental impacts of Hanford operations and calculate the overall radiological dose to humans onsite, at the Site perimeter, or residing in nearby communities. Chinook salmon (Oncorhynchus tshawytscha) spawning in the Columbia River at Hanford has increased in recent years with a concomitant increase in winter nesting activity of bald eagles (Haliaeetus leucocephalus). An elk (Cervus elaphus) herd, established by immigration in 1972, is also increasing. Nesting Canada goose (Branta canadensis) and great blue heron (Ardea herodias), and various other animals, e.g., mule deer (Odocoileus hemionus) and coyotes (Canis latrans) are common. Measured exposure to penetrating radiation and calculated radiation doses to the public are well below applicable regulatory limits. 35 refs., 4 figs

  7. Environmental assessment: Reference repository location, Hanford site, Washington

    Energy Technology Data Exchange (ETDEWEB)

    none,

    1986-05-01

    In February 1983, the US Department of Energy (DOE) identified a reference repository location at the Hanford Site in Washington as one of the nine potentially acceptable sites for a mined geologic repository for spent nuclear fuel and high-level radioactive waste. The site is in the Columbia Plateau, which is one of five distinct geohydrologic settings considered for the first repository. To determine their suitability, the Hanford Site and the eight other potentially acceptable sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. These evaluations were reported in draft environmental assessments (EAs), which were issued for public review and comment. After considering the comments received on the draft EAs, the DOE prepared the final EAs. On the basis of the evaluations reported in this EA, the DOE has found that the Hanford site is not disqualified under the guidelines. The DOE has also found that it is suitable for site characterization because the evidence does not support a conclusion that the site will not be able to meet each of the qualifying conditions specified in the guidelines. On the basis of these findings, the DOE is nominating the Hanford site as one of five sites suitable for characterization.

  8. Environmental assessment: Reference repository location, Hanford site, Washington

    Energy Technology Data Exchange (ETDEWEB)

    none,

    1986-05-01

    In February 1983, the US Department of Energy (DOE) identified a reference repository location at the Hanford Site in Washington as one of the nine potentially acceptable sites for a mined geologic repository for spent nuclear fuel and high-level radioactive waste. The site is in the Columbia Plateau, which is one of five distinct geohydrologic settings considered for the first repository. To determine their suitability, the Hanford site and the eight other potentially acceptable sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. These evaluations were reported in draft environmental assessments (EAs), which were issued for public review and comment. After considering the comments received on the draft EAs, the DOE prepared the final EAs. On the basis of the evaluations reported in this EA, the DOE has found that the Hanford site is not disqualified under the guidelines. The DOE has also found that is is suitable for site characterization because the evidence does not support a conclusion that the site will not be able to meet each of the qualifying conditions specified in the guidelines. On the basis of these findings, the DOE is nominating the Hanford site as one of five sites available for characterization.

  9. Preliminary geology of eastern Umtanum Ridge, South-Central Washington

    International Nuclear Information System (INIS)

    Goff, F.E.

    1981-01-01

    The basalt stratigraphy and geologic structures of eastern Umtanum Ridge have been mapped and studied in detail to help assess the feasibility of nuclear waste terminal storage on the Hanford Site in southeastern Washington State. Eastern Umtanum Ridge is an asymmetric east-west-trending anticline of Columbia River basalt that plunges 5 degrees eastward into the Pasco Basin. Geologic mapping and determination of natural remanent magnetic polarity and chemical composition reveal that flows of the Pomona and Umatilla Members (Saddle Mountains Basalt), Priest Rapids and Frenchman Springs Members (Wanapum Basalt), and Grande Ronde Basalt were erupted as fairly uniform sheets. The Wahluke and Huntzinger flows (Saddle Mountains Basalt) fill a paleovalley cut into Wanapum Basalt. No evidence was found to indicate Quaternary-age movement on any structures in the map area. The basalt strata on the south limb of the Umtanum anticline display relatively little tectonic deformation since Miocene-Pliocene time. Thus, the buried south flank of Umtanum Ridge may provide an excellent location for a nuclear waste repository beneath the Hanford Site

  10. Environmental assessment: Reference repository location, Hanford site, Washington

    International Nuclear Information System (INIS)

    1986-05-01

    In February 1983, the US Department of Energy (DOE) identified a reference repository location at the Hanford Site in Washington as one of the nine potentially acceptable sites for a mined geologic repository for spent nuclear fuel and high-level radioactive waste. The site is in the Columbia Plateau, which is one of five distinct geohydrologic settings considered for the first repository. To determine their suitability, the Hanford Site and the eight other potentially acceptable sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. These evaluations were reported in draft environmental assessments (EAs), which were issued for public review and comment. After considering the comments received on the draft EAs, the DOE prepared the final EAs. On the basis of the evaluations reported in this EA, the DOE has found that the Hanford site is not disqualified under the guidelines. The DOE has also found that it is suitable for site characterization because the evidence does not support a conclusion that the site will not be able to meet each of the qualifying conditions specified in the guidelines. On the basis of these findings, the DOE is nominating the Hanford site as one of five sites suitable for characterization

  11. Environmental assessment: Reference repository location, Hanford site, Washington

    International Nuclear Information System (INIS)

    1986-05-01

    In February 1983, the US Department of Energy (DOE) identified a reference repository location at the Hanford Site in Washington as one of the nine potentially acceptable sites for a mined geologic repository for spent nuclear fuel and high-level radioactive waste. The site is in the Columbia Plateau, which is one of five distinct geohydrologic settings considered for the first repository. To determine their suitability, the Hanford site and the eight other potentially acceptable sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. These evaluations were reported in draft environmental assessments (EAs), which were issued for public review and comment. After considering the comments received on the draft EAs, the DOE prepared the final EAs. On the basis of the evaluations reported in this EA, the DOE has found that the Hanford site is not disqualified under the guidelines. The DOE has also found that is is suitable for site characterization because the evidence does not support a conclusion that the site will not be able to meet each of the qualifying conditions specified in the guidelines. On the basis of these findings, the DOE is nominating the Hanford site as one of five sites available for characterization

  12. Images and Memories: photography as document and research source for the historical recovery of Colônia Esperança (1935-1963 Imagens e Memórias: a fotografia como documento e fonte de pesquisa para a recuperação histórica da Colônia Esperança (1935-1963

    Directory of Open Access Journals (Sweden)

    Larissa Ayumi Sato

    2011-08-01

    Full Text Available The use of photography as research source and historical document has been set as important instrument in the process of historical recovery of recent settlement places as Colônia Esperança, rural community located in the city of Arapongas, on the border of Apucarana. This study considers the rescue of this community’s ancient images and the search of details that can contextualize this pictures become important tools for the recovery of scenarios that nowadays are modified by the passage of the time. In the case of Colônia Esperança, that appeared as a redoubt of catholic Japanese people searching for more fertile lands and better life conditions, around a common ideology (keep their religious practices and the customs of their origin country, this kind of analysis become particularly relevant due to the history of this location is not properly systematized. The colony was founded in 1935, even before the city of Arapongas, that was started in 1937, product of colonization works made by Companhia de Terras Norte do Paraná. This sense, this research uses as methodological procedures the reading, the iconographic analysis and the iconological interpretation of photography’s elements, bibliographic and documentary research, in addition to the oral history – as technique – to salvage colonization and everyday life aspects in the initial decades (30’s to 60’s in the location. In the end of this project, it’s expected the obtained systematization about the history of Colônia Esperança, enriched with rare pictures that were in hand of the pioneer families, unpublished testimonials and documents, fundamental for this process, can contribute to evidence the role of the photography as historical document and research source. A utilização de fotografias como fonte de pesquisa e documento histórico tem se configurado como importante instrumento no processo de reconstituição da história de lugares de coloniza-ção recente, como a

  13. Expressão citofotométrica da Caspase-3 no carcinoma papilífero da tireóide e no bócio colóide

    Directory of Open Access Journals (Sweden)

    Luciana Rodrigues Queiroz de Souza

    Full Text Available OBJETIVO: Descrever a expressão citofotométrica quantitativa do marcador caspase-3 no bócio colóide e no carcinoma papilífero da tireóide e comparar a imunoexpessão entre as doenças. MÉTODOS: Realizou-se estudo imunoistoquímico da proteína caspase-3 em 17 blocos de parafina de carcinoma papilífero da tiróide e 20 de bócio colóide, através do sistema SAMBA 4000 - (Sistema de análise microscópica de busca automática, objetivando-se analisar duas variáveis: índice de marcagem e densidade óptica. RESULTADOS: Houve diferença significativa quanto ao índice de marcagem da caspase-3, entre o bócio colóide e o carcinoma papilífero, sendo maior no carcinoma, e não foi encontrada diferença significativa quanto à densidade óptica. Para o bócio colóide, o coeficiente de correlação estimado entre o índice de marcagem e a densidade óptica foi igual a 0,72, indicando assim, a rejeição da hipótese nula (p <0,001, afirmando-se que existe associação positiva e significativa entre o índice de marcagem e a densidade óptica da caspase-3. Para o carcinoma papilífero da tiróide, o coeficiente de correlação estimado entre o índice de marcagem e a densidade óptica 3 foi de 0,34. O resultado do teste estatístico indicou que não se pode afirmar que existe associação entre esses parâmetros. CONCLUSÃO: Para o bócio colóide existe associação positiva e significativa entre as duas variáveis, índice de marcagem e a densidade óptica da caspase-3, enquanto que para o carcinoma papilífero não existe essa associação. O estudo comparativo entre a análise quantitativa da caspase-3, demonstrou que a apoptose é mais evidente no carcinoma papilífero do que no bócio colóide.

  14. Sulfates, Clouds and Radiation Brazil (SCAR-B) University of Washington C131A Data

    Data.gov (United States)

    National Aeronautics and Space Administration — SCAR_B_UWC131A data are Smoke/Sulfates, Clouds and Radiation Experiment in Brazil data from instruments on board the University of Washington C131A aircraft in...

  15. Relation between Nitrates in Water Wells and Potential Sources in the Lower Yakima Valley, Washington State

    Science.gov (United States)

    Results of a study EPA conducted to investigate the contribution of various sources to the high nitrate levels in groundwater and residential drinking water wells in the Lower Yakima Valley of Washington State.

  16. Aerial Photography and Imagery, Ortho-Corrected - 2007 Digital Orthophotos - Washington County

    Data.gov (United States)

    NSGIC Education | GIS Inventory — This dataset is a collection of GeoTIFF and MrSID format natural color orthophotos covering Washington, Holmes, and Bay County, Florida. An orthophoto is remotely...

  17. 75 FR 434 - Notice of Intent to Repatriate Cultural Items: Thomas Burke Memorial Washington State Museum...

    Science.gov (United States)

    2010-01-05

    ... bone handle. The funerary objects were removed from the area surrounding Lake Washington primarily on... the 1870s, as the City of Seattle developed, the Lake people were pushed out to other areas, including...

  18. Partners In Motion And Customer Satisfaction In The Washington Dc Metropolitan Area

    Science.gov (United States)

    1999-06-01

    PARTNERS IN MOTION IS A PROGRAM AIMED AT IMPROVING THE QUALITY, QUANTITY, AND AVAILABILITY OF TRAVEL INFORMATION TO TRANSPORTATION AGENCIES, THE MEDIA, AND, ULTIMATELY, TO THE TRAVELER IN THE WASHINGTON, D.C. METROPOLITAN AREA. THE PROGRAM WAS INITIA...

  19. 78 FR 59955 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

    Science.gov (United States)

    2013-09-30

    ... responsibilities under NAGPRA, 25 U.S.C. 3003(d)(3). The determinations in this notice are the sole responsibility... Reservation, Washington) that this notice has been published. Dated: August 20, 2013. Sherry Hutt, Manager...

  20. Washington State Briefing Book for low-level radioactive waste management

    International Nuclear Information System (INIS)

    1980-12-01

    The Washington State Briefing Book is one of a series of state briefing books on low-level radioactive waste management practices. It has been prepared to assist state and federal agency officials in planning for safe low-level radioactive waste disposal. The report contains a profile of low-level radioactive waste generators in Washington. The profile is the result of a survey of NRC licensees in Washington. The briefing book also contains a comprehensive assessment of low-level radioactive waste management issues and concerns as defined by all major interested parties including industry, government, the media, and interest groups. The assessment was developed through personal communications with representatives of interested parties, and through a review of media sources. Lastly, the briefing book provides demographic and socioeconomic data and a discussion of relevant government agencies and activities, all of which may impact waste management practices in Washington