Colágeno na cartilagem osteoartrótica

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Ana Paula P. Velosa

2003-06-01

Full Text Available A cartilagem articular é um tecido altamente especializado, composto por células, os condrócitos, e um conjunto de macromoléculas, como o colágeno e os proteoglicanos. O colágeno é uma proteína fibrilar que garante resistência ao tecido, enquanto os proteoglicanos têm a função de mola biológica, sendo responsáveis pela compressibilidade da cartilagem. A complexa interação entre estas duas proteínas garante a elasticidade. Estas características específicas da cartilagem são essenciais para amortecer as grandes forças de impacto a que as articulações diartrodiais estão submetidas, sem muito gasto de energia, visto tratar-se de um tecido avascular. Em processos artrósicos ocorre um desequilíbrio entre a produção de componentes da matriz extracelular e destruição pelas metaloproteases, levando à degradação e perda do tecido cartilaginoso. A fase inicial da osteoartrose é marcada por perda de fragmentos de proteoglicanos para o líquido sinovial, aumento dos colágenos tipo II e tipo VI, aparecimento dos colágenos I e III, não típicos da cartilagem, e diminuição do colágeno tipo IX, que é importante para manter a integridade da matriz extracelular, além do entumescimento da cartilagem. Como conseqüência, a cartilagem perde suas características específicas, levando a alterações na função articular. A evolução da doença promove diminuição significativa das proteínas, até mesmo do colágeno tipo XI, que tem localização mais interna na estrutura da fibrila heterotípica, e, portanto levando até a exposição do osso. Até o momento, o tratamento da osteoartrose está baseado principalmente no controle da dor e/ou inflamação, não diminuindo ou impedindo a degradação da cartilagem articular. Neste aspecto a perspectiva de tratamento futuro da osteoartrose estaria na utilização de inibidores das metaloproteases associadas a condroprotetores interferindo no "turnover" da cartilagem e

Abortive phage-infection and UV-protection markers on ColI plasmids from epidemic strains of Salmonella

International Nuclear Information System (INIS)

Barker, R.M.

1988-01-01

Cultures of Escherichia coli carrying ColI plasmids received in conjugation from strains of Salmonella typhimurium and S. agona were examined for abortive infection (Abi) of phage BF23 and for enhanced resistance to the lethal action of UV-irradiation (Uvr). The Abi character of stored cultures of E. coli was also compared with the reaction of the same stock culture tested 5 years before. Seven of the eight potential types differentiated by three characters were represented among 160 ColI plasmids: ColIa Abi + Uvr + (3 plasmids), ColIa Abi - Uvr + (1), ColIa Abi - Uvr-> (2), ColIb Abi + Uvr + (85), ColIb Abi + Uvr - (5), ColIb Abi - Uvr + (4), ColIb Abi-? Uvr - (60). Recognition that different plasmid types could be carried by strains of a clone proved useful in the interpretation of the epidemic spread of strains of S. typhimurium of phage type/biotype 141/9f in Scotland and in tracing the ancestry of a recently emerged rhamnose non-fermenting mutant strain of S. agona. (author)

COL11A1 in FAP polyps and in sporadic colorectal tumors

International Nuclear Information System (INIS)

Fischer, Heléne; Salahshor, Sima; Stenling, Roger; Björk, Jan; Lindmark, Gudrun; Iselius, Lennart; Rubio, Carlos; Lindblom, Annika

2001-01-01

We previously reported that the α-1 chain of type 11 collagen (COL11A1), not normally expressed in the colon, was up-regulated in stromal fibroblasts in most sporadic colorectal carcinomas. Patients with germline mutations in the APC gene show, besides colonic polyposis, symptoms of stromal fibroblast involvement, which could be related to COL11A1 expression. Most colorectal carcinomas are suggested to be a result of an activated Wnt- pathway, most often involving an inactivation of the APC gene or activation of β-catenin. We used normal and polyp tissue samples from one FAP patient and a set of 37 sporadic colorectal carcinomas to find out if the up-regulation of COL11A1 was associated with an active APC/β-catenin pathway. In this study we found a statistically significant difference in COL11A1 expression between normal tissue and adenomas from one FAP patient, and all adenomas gave evidence for an active APC/β-catenin pathway. An active Wnt pathway has been suggested to involve stromal expression of WISP-1. We found a strong correlation between WISP-1 and COL11A1 expression in sporadic carcinomas. Our results suggest that expression of COL11A1 in colorectal tumors could be associated with the APC/β-catenin pathway in FAP and sporadic colorectal cancer

Scoliosis in osteogenesis imperfecta caused by COL1A1/COL1A2 mutations - genotype-phenotype correlations and effect of bisphosphonate treatment.

Science.gov (United States)

Sato, Atsuko; Ouellet, Jean; Muneta, Takeshi; Glorieux, Francis H; Rauch, Frank

2016-05-01

Bisphosphonates are widely used to treat children with osteogenesis imperfecta (OI), a bone fragility disorder that is most often caused by mutations in COL1A1 or COL1A2. However, it is unclear whether this treatment decreases the risk of developing scoliosis. We retrospectively evaluated spine radiographs and charts of 437 patients (227 female) with OI caused by mutations in COL1A1 or COL1A2 and compared the relationship between scoliosis, genotype and bisphosphonate treatment history. At the last follow-up (mean age 11.9 [SD: 5.9] years), 242 (55%) patients had scoliosis. The prevalence of scoliosis was highest in OI type III (89%), followed by OI type IV (61%) and OI type I (36%). Moderate to severe scoliosis (Cobb angle ≥25°) was rare in individuals with COL1A1 haploinsufficiency mutations but was present in about two fifth of patients with triple helical glycine substitutions or C-propeptide mutations. During the first 2 to 4years of bisphosphonate therapy, patients with OI type III had lower Cobb angle progression rates than before bisphosphonate treatment, whereas in OI types I and IV bisphosphonate treatment was not associated with a change in Cobb angle progression rates. At skeletal maturity, the prevalence of scoliosis (Cobb angle >10°) was similar in patients who had started bisphosphonate treatment early in life (before 5.0years of age) and in patients who had started therapy later (after the age of 10.0years) or had never received bisphosphonate therapy. Bisphosphonate treatment decreased progression rate of scoliosis in OI type III but there was no evidence of a positive effect on scoliosis in OI types I and IV. The prevalence of scoliosis at maturity was not influenced by the bisphosphonate treatment history in any OI type. Copyright © 2016 Elsevier Inc. All rights reserved.

2002 Industry Studies: News Media

Science.gov (United States)

2002-01-01

USAF CAPT John W. Coleman II, USN Reserve Col Christos Dellas , Greek Army Col Tim Fletcher USAF Lt Col Tim Malinski USAF Lt Col Deb Martinez USAF Ms...Majority Support Government Restrictions on War Coverage,” Washington Post, 29 November 2001, Sec. C, p.1. 54 Lucia Moses, “E&P Survey: Circ Bump was...Afghan War,” The Washington Post, 7 December 2001, Sec. A, p. 43. Moses, Lucia , “E&P Survey: Circ Bump was Modest,” Editor & Publisher, 22 April 2002

Entomopathogenic fungi in predatory beetles (Col: Carabidae and Staphylinidae) from agricultural fields

DEFF Research Database (Denmark)

Steenberg, T; Langer, V; Esbjerg, P

1995-01-01

Prevalence of entomopathogenic fungi was studied in overwintering ground beetles (Col.: Carabidae) and rove beetles (Col.: Staphylinidae) collected from fields of lucerne, white cabbage and white cabbage undersown with white clover. In general infection levels in adult ground beetles and rove bee...

Evaluation the COL9A2 gene with high myopia

Science.gov (United States)

Zhang, Dingding; Huang, Maomin

2017-11-01

This paper investigates the association of the COL9A2 gene between high myopia and normal controls in the Han Chinese population. It shows that the frameshift mutation (D281fs) in the COL9A2 gene is not associated with high myopia in the Han Chinese population, and the two novel variants(c.143G＞C and c.884G＞A) may contribute to the development of high myopia.

Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNA

Science.gov (United States)

Hershfield, Vickers; Boyer, Herbert W.; Yanofsky, Charles; Lovett, Michael A.; Helinski, Donald R.

1974-01-01

DNA fragments obtained from EcoRI endonuclease digestion of bacteriophage ϕ80pt190 (trp+) and the plasmid ColE1 were covalently joined with polynucleotide ligase. Transformation of Escherichia coli trp- strains to tryptophan independence with the recombined DNA selected for reconstituted ColE1 plasmids containing the tryptophan operon and the ϕ80 immunity region. Similarly, an EcoRI endonuclease generated fragment of plasmid pSC105 DNA containing the genetic determinant of kanamycin resistance was inserted into the ColE1 plasmid and recovered in E. coli. The plasmids containing the trp operon (ColE1-trp) and the kanamycin resistance gene were maintained under logarithmic growth conditions at a level of 25-30 copies per cell and accumulate to the extent of several hundred copies per cell in the presence of chloramphenicol. Cells carrying the ColE1-trp plasmid determined the production of highly elevated levels of trp operon-specific mRNA and tryptophan biosynthetic enzymes. Images PMID:4610576

TUMOR-SPECIFIC EXPRESSION AND ALTERNATIVE SPLICING OF THE COL6A3 GENE IN PANCREATIC CANCER

Science.gov (United States)

Arafat, Hwyda; Lazar, Melissa; Salem, Khalifa; Chipitsyna, Galina; Gong, Qiaoke; Pan, Te-Cheng; Zhang, Rui-Zhu; Yeo, Charles J.; Chu, Mon-Li

2011-01-01

Introduction Pancreatic ductal adenocarcinoma (PDA) is a highly lethal disease in which a prominent desmoplastic reaction is a defining characteristic. Fibrillar collagens, such as collagen I and to a lesser extent, collagen III and V comprise the majority of this stromal fibrosis. Type VI collagen (COL6) forms a microfibrillar network associated with type I collagen fibrils. The expression of COL6 has been linked to inflammation and survival. Importantly, tumor-specific alternative splicing in COL6A3 has been identified in several cancers by genome exon arrays. We evaluated the expression and localization of COL6A3 in PDA and premalignant lesions and explored the presence of alternative splicing events. Methods We analyzed paired PDA-normal (n=18), IPMN (n=5), pancreatic cystadenoma (n=5), and eight PDA cell lines with RT-PCR, using unique primers that identify total COL6A3 gene and alternative splicing sites in several of its exons. Western blot analysis and immunohistochemistry were used to analyze the expression levels and localization of COL6A3 protein in the different lesions, and in two animal models of PDA. Results COL6A3 protein levels were significantly upregulated in 77% of the paired PDA-adjacent tissue examined. COL6A3 was mainly present in the desmoplastic stroma of PDA, with high deposition around the malignant ducts and in between the sites of stromal fatty infiltration. Analysis of the COL6A3 splice variants showed tumor-specific consistent inclusion of exons 3 and 6 in 17 of the 18 (94%) paired PDA-adjacent tissues. Inclusion of exon 4 was exclusively tumor-specific, with barely detectable expression in the adjacent tissues. IPMN and pancreatic cystadenomas showed no expression of any of the examined exons. Total COL6A3 mRNA and exon 6 were identified in six PDA cell lines, but only two cell lines (MIA PACA-2 and ASPC-1) expressed exons 3 and 4. In both the xenograft and transgenic models of PDA, COL6A3 immunoreactivity was present in the stroma

A novel COL4A3 mutation causes autosomal-recessive Alport syndrome in a large Turkish family.

Science.gov (United States)

Uzak, Asli Subasioglu; Tokgoz, Bulent; Dundar, Munis; Tekin, Mustafa

2013-03-01

Alport syndrome (AS) is a genetically heterogeneous disorder that is characterized by hematuria, progressive renal failure typically resulting in end-stage renal disease, sensorineural hearing loss, and variable ocular abnormalities. Only 15% of cases with AS are autosomal recessive and are caused by mutations in the COL4A3 or COL4A4 genes, encoding type IV collagen. Clinical data in a large consanguineous family with four affected members were reviewed, and genomic DNA was extracted. For mapping, 15 microsatellite markers flanking COL4A3, COL4A4, and COL4A5 in 16 family members were typed. For mutation screening, all coding exons of COL4A3 were polymerase chain reaction- amplified and Sanger-sequenced from genomic DNA. The disease locus was mapped to chromosome 2q36.3, where COL4A3 and COL4A4 reside. Sanger sequencing revealed a novel mis-sense mutation (c.2T>C; p.M1T) in exon 1 of COL4A3. The identified nucleotide change was not found in 100 healthy ethnicity-matched controls via Sanger sequencing. We present a large consanguineous Turkish family with AS that was found to have a COL4A3 mutation as the cause of the disease. Although the relationship between the various genotypes and phenotypes in AS has not been fully elucidated, detailed clinical and molecular analyses are helpful for providing data to be used in genetic counseling. It is important to identify new mutations to clarify their clinical importance, to assess the prognosis of the disease, and to avoid renal biopsy for final diagnosis.

Mutations in COL1A1 and COL1A2 and dental aberrations in children and adolescents with osteogenesis imperfecta – A retrospective cohort study

Science.gov (United States)

Dahllöf, Göran; Lindahl, Katarina; Kindmark, Andreas; Grigelioniene, Giedre; Åström, Eva; Malmgren, Barbro

2017-01-01

Osteogenesis imperfecta (OI) is a heterogeneous group of disorders of connective tissue, caused mainly by mutations in the collagen I genes (COL1A1 and COL1A2). Dentinogenesis imperfecta (DGI) and other dental aberrations are common features of OI. We investigated the association between collagen I mutations and DGI, taurodontism, and retention of permanent second molars in a retrospective cohort of 152 unrelated children and adolescents with OI. The clinical examination included radiographic evaluations. Teeth from 81 individuals were available for histopathological evaluation. COL1A1/2 mutations were found in 104 individuals by nucleotide sequencing. DGI was diagnosed clinically and radiographically in 29% of the individuals (44/152) and through isolated histological findings in another 19% (29/152). In the individuals with a COL1A1 mutation, 70% (7/10) of those with a glycine substitution located C-terminal of p.Gly305 exhibited DGI in both dentitions while no individual (0/7) with a mutation N-terminal of this point exhibited DGI in either dentition (p = 0.01). In the individuals with a COL1A2 mutation, 80% (8/10) of those with a glycine substitution located C terminal of p.Gly211 exhibited DGI in both dentitions while no individual (0/5) with a mutation N-terminal of this point (p = 0.007) exhibited DGI in either dentition. DGI was restricted to the deciduous dentition in 20 individuals. Seventeen had missense mutations where glycine to serine was the most prevalent substitution (53%). Taurodontism occurred in 18% and retention of permanent second molars in 31% of the adolescents. Dental aberrations are strongly associated with qualitatively changed collagen I. The varying expressivity of DGI is related to the location of the collagen I mutation. Genotype information may be helpful in identifying individuals with OI who have an increased risk of dental aberrations. PMID:28498836

Persistent Notochord in a Fetus with COL2A1 Mutation

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Elisabeth Codsi

2015-01-01

Full Text Available Multiple anomalies including micromelia, poor mineralization of the vertebrae, and a persistent notochord were identified on second trimester ultrasound in a fetus with a COL2A1 mutation. To our knowledge, this represents the first case of a persistent notochord associated with a COL2A1 mutation in humans. In this case report, we describe ultrasound and postmortem findings and review the pathogenesis associated with a persistent notochord.

Overexpression of COL11A1 by cancer-associated fibroblasts: clinical relevance of a stromal marker in pancreatic cancer.

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Carmen García-Pravia

Full Text Available BACKGROUND: The collagen11A1 (COL11A1 gene is overexpressed in pancreatic cancer. The expression of COL11A1 protein could be involved in desmoplastic events in pancreatic cancer, but an antibody that specifically stains the COL11A1 protein is not currently available. METHODS AND FINDINGS: A total of 54 pancreatic ductal adenocarcinomas (PDAC, 23 chronic pancreatitis (CP samples, and cultured peritumoral stromal cells of PDAC (passages 3-6 were studied. Normal human pancreas tissue samples were obtained through a cadaveric organ donation program. 1 Validation of COL11A1 gene overexpression by q-RT-PCR. FINDINGS: the expression of COL11A1 gene is significantly increased in PDAC samples vs. normal and CP samples. 2 Analysis of COL11A1 by immunohistochemistry using highly specific anti-proCOL11A1 antibodies. FINDINGS: anti-proCOL11A1 stains stromal cells/cancer-associated fibroblasts (CAFs of PDAC but it does not stain chronic benign condition (chronic pancreatitis stromal cells, epithelial cells, or normal fibroblasts. 3 Evaluation of the discrimination ability of the antibody. FINDINGS: anti-proCOL11A1 immunostaining accurately discriminates between PDAC and CP (AUC 0.936, 95% CI 0.851, 0.981. 4 Phenotypic characterization of proCOL11A1+ stromal cells co-staining with mesenchymal, epithelial and stellate cell markers on pancreatic tissue samples and cultured peritumoral pancreatic cancer stromal cells. FINDINGS: ProCOL11A1+ cells present co-staining with mesenchymal, stellate and epithelial markers (EMT phenotype in different proportions. CONCLUSIONS/SIGNIFICANCE: Detection of proCOL11A1 through immunostaining with this newly-developed antibody allows for a highly accurate distinction between PDAC and CP. Unlike other available antibodies commonly used to detect CAFs, anti-proCOL11A1 is negative in stromal cells of the normal pancreas and almost absent in benign inflammation. These results strongly suggest that proCOL11A1 is a specific marker for

Mutations in COL1A1 and COL1A2 and dental aberrations in children and adolescents with osteogenesis imperfecta - A retrospective cohort study.

Directory of Open Access Journals (Sweden)

Kristofer Andersson

Full Text Available Osteogenesis imperfecta (OI is a heterogeneous group of disorders of connective tissue, caused mainly by mutations in the collagen I genes (COL1A1 and COL1A2. Dentinogenesis imperfecta (DGI and other dental aberrations are common features of OI. We investigated the association between collagen I mutations and DGI, taurodontism, and retention of permanent second molars in a retrospective cohort of 152 unrelated children and adolescents with OI. The clinical examination included radiographic evaluations. Teeth from 81 individuals were available for histopathological evaluation. COL1A1/2 mutations were found in 104 individuals by nucleotide sequencing. DGI was diagnosed clinically and radiographically in 29% of the individuals (44/152 and through isolated histological findings in another 19% (29/152. In the individuals with a COL1A1 mutation, 70% (7/10 of those with a glycine substitution located C-terminal of p.Gly305 exhibited DGI in both dentitions while no individual (0/7 with a mutation N-terminal of this point exhibited DGI in either dentition (p = 0.01. In the individuals with a COL1A2 mutation, 80% (8/10 of those with a glycine substitution located C terminal of p.Gly211 exhibited DGI in both dentitions while no individual (0/5 with a mutation N-terminal of this point (p = 0.007 exhibited DGI in either dentition. DGI was restricted to the deciduous dentition in 20 individuals. Seventeen had missense mutations where glycine to serine was the most prevalent substitution (53%. Taurodontism occurred in 18% and retention of permanent second molars in 31% of the adolescents. Dental aberrations are strongly associated with qualitatively changed collagen I. The varying expressivity of DGI is related to the location of the collagen I mutation. Genotype information may be helpful in identifying individuals with OI who have an increased risk of dental aberrations.

A COL11A2 mutation in Labrador retrievers with mild disproportionate dwarfism.

Science.gov (United States)

Frischknecht, Mirjam; Niehof-Oellers, Helena; Jagannathan, Vidhya; Owczarek-Lipska, Marta; Drögemüller, Cord; Dietschi, Elisabeth; Dolf, Gaudenz; Tellhelm, Bernd; Lang, Johann; Tiira, Katriina; Lohi, Hannes; Leeb, Tosso

2013-01-01

We describe a mild form of disproportionate dwarfism in Labrador Retrievers, which is not associated with any obvious health problems such as secondary arthrosis. We designate this phenotype as skeletal dysplasia 2 (SD2). It is inherited as a monogenic autosomal recessive trait with incomplete penetrance primarily in working lines of the Labrador Retriever breed. Using 23 cases and 37 controls we mapped the causative mutation by genome-wide association and homozygosity mapping to a 4.44 Mb interval on chromosome 12. We re-sequenced the genome of one affected dog at 30x coverage and detected 92 non-synonymous variants in the critical interval. Only two of these variants, located in the lymphotoxin A (LTA) and collagen alpha-2(XI) chain gene (COL11A2), respectively, were perfectly associated with the trait. Previously described COL11A2 variants in humans or mice lead to skeletal dysplasias and/or deafness. The dog variant associated with disproportionate dwarfism, COL11A2:c.143G>C or p.R48P, probably has only a minor effect on collagen XI function, which might explain the comparatively mild phenotype seen in our study. The identification of this candidate causative mutation thus widens the known phenotypic spectrum of COL11A2 mutations. We speculate that non-pathogenic COL11A2 variants might even contribute to the heritable variation in height.

COL Application Content Guide for HTGRs: Revision to RG 1.206, Part 1 - Status Report

Energy Technology Data Exchange (ETDEWEB)

Wayne Moe

2012-08-01

A combined license (COL) application is required by the Nuclear Regulatory Commission (NRC) for all proposed nuclear plants. The information requirements for a COL application are set forth in 10 CFR 52.79, “Contents of Applications; Technical Information in Final Safety Analysis Report.” An applicant for a modular high temperature gas-cooled reactor (HTGR) must develop and submit for NRC review and approval a COL application which conforms to these requirements. The technical information necessary to allow NRC staff to evaluate a COL application and resolve all safety issues related to a proposed nuclear plant is detailed and comprehensive. To this, Regulatory Guide (RG) 1.206, “Combined License Applications for Nuclear Power Plants” (LWR Edition), was developed to assist light water reactor (LWR) applicants in incorporating and effectively formatting required information for COL application review (Ref. 1). However, the guidance prescribed in RG 1.206 presumes a LWR design proposal consistent with the systems and functions associated with large LWR power plants currently operating under NRC license.

CHROMagar COL-APSE: a selective bacterial culture medium for the isolation and differentiation of colistin-resistant Gram-negative pathogens

DEFF Research Database (Denmark)

Abdul Momin, Muhd Haziq F; Bean, David C; Hendriksen, Rene S.

2017-01-01

medium (SuperPolymyxin). Methodology. The medium was challenged with 84 isolates, including polymyxin B (POL B)-susceptible and -resistant type strains and colistin (COL)-resistant organisms recovered from human and animal samples. Susceptibility to COL and POL B was determined by agar dilution and broth...... microtitre dilution. The lower limit for the detection of COL-resistant organisms was also calculated for both CHROMagar COL-APSE and SuperPolymyxin media. The ability to isolate and correctly differentiate COL-resistant organisms within mixed cultures was also assessed and compared using both media. Results...

A COL11A2 mutation in Labrador retrievers with mild disproportionate dwarfism.

Directory of Open Access Journals (Sweden)

Mirjam Frischknecht

Full Text Available We describe a mild form of disproportionate dwarfism in Labrador Retrievers, which is not associated with any obvious health problems such as secondary arthrosis. We designate this phenotype as skeletal dysplasia 2 (SD2. It is inherited as a monogenic autosomal recessive trait with incomplete penetrance primarily in working lines of the Labrador Retriever breed. Using 23 cases and 37 controls we mapped the causative mutation by genome-wide association and homozygosity mapping to a 4.44 Mb interval on chromosome 12. We re-sequenced the genome of one affected dog at 30x coverage and detected 92 non-synonymous variants in the critical interval. Only two of these variants, located in the lymphotoxin A (LTA and collagen alpha-2(XI chain gene (COL11A2, respectively, were perfectly associated with the trait. Previously described COL11A2 variants in humans or mice lead to skeletal dysplasias and/or deafness. The dog variant associated with disproportionate dwarfism, COL11A2:c.143G>C or p.R48P, probably has only a minor effect on collagen XI function, which might explain the comparatively mild phenotype seen in our study. The identification of this candidate causative mutation thus widens the known phenotypic spectrum of COL11A2 mutations. We speculate that non-pathogenic COL11A2 variants might even contribute to the heritable variation in height.

Diferença no tamanho de cascas de ovos de colônias de Panstrongylus megistus

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Rosa João A da

2003-01-01

Full Text Available O objetivo do estudo foi avaliar as mensurações de cascas de ovos de duas colônias laboratoriais e uma silvestre de P. megistus. De cada uma das colônias, 50 cascas de ovos foram mensuradas por meio de microscópio estereoscópico e sistema de análise de imagem QWin. A análise estatística foi efetuada utilizando o teste t-Student, não pareado no programa INSTAT. A largura, o comprimento e o diâmetro da abertura opercular das cascas de ovos de uma colônia silvestre foram maiores que de duas colônias laboratoriais de P. megistus.

An apple B-box protein, MdCOL11, is involved in UV-B- and temperature-induced anthocyanin biosynthesis.

Science.gov (United States)

Bai, Songling; Saito, Takanori; Honda, Chikako; Hatsuyama, Yoshimichi; Ito, Akiko; Moriguchi, Takaya

2014-11-01

Our studies showed that an apple B-box protein, MdCOL11, the homolog of AtBBX22, is involved in UV-B- and temperature-induced anthocyanin biosynthesis in apple peel. Anthocyanin is responsible for the red pigmentation in apple peel and a R2R3 MYB gene, MdMYBA/1/10, a homolog of MdMYBA, controls its accumulation. Arabidopsis PAP1 is under the control of a series of upstream factors involved in light signal transduction and photomorphogenesis, such as ELONGATED HYPOCOTYL 5 (HY5) and B-box family (BBX) proteins. In this study, we identified and characterized the homolog of Arabidopsis BBX22 in apple, designated as MdCOL11. Overexpression of MdCOL11 in Arabidopsis enhanced the accumulation of anthocyanin. In apples, MdCOL11 was differentially expressed in all tissues, with the highest expression in petals and the lowest expression in the xylem. Transcripts of MdCOL11 noticeably accumulated at the ripening stage, concomitant with increases in the expressions of anthocyanin biosynthesis-related genes. In an in vitro treatment experiment, MdCOL11 was upregulated in an ultra-violet (UV)-B- and temperature-dependent manner, together with the inductions of anthocyanin biosynthesis-related genes and anthocyanin accumulation in apple peel. Furthermore, a dual-luciferase assay indicated that (1) MdCOL11 regulated the expression of MdMYBA and (2) MdCOL11 was a target of MdHY5. Taken together, our results suggest that MdCOL11 is involved in MdHY5-mediated signal transduction and regulates anthocyanin accumulation in apple peel, which sheds new light on anthocyanin accumulation in apples.

Case of Small Vessel Disease Associated with COL4A1 Mutations following Trauma

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Joao McONeil Plancher

2015-06-01

Full Text Available With this case report, we would like to heighten the awareness of clinicians about COL4A1 as a single-gene disorder causing cerebral small vessel disease and describe a previously unreported pathogenic missense substitution in COL4A1 (p.Gly990Val and a new clinical presentation. We identified a heterozygous putatively pathogenic mutation of COL4A1 in a 50-year-old female with a history of congenital cataracts and glaucoma who presented with multiple diffusion-positive infarcts and areas of contrast enhancement following mild head trauma. We believe that this presentation of multiple areas of acute brain and vascular injury in the setting of mild head trauma is a new manifestation of this genetic disorder. Imaging findings of multiple acute infarcts and regions of contrast enhancement with associated asymptomatic old deep microhemorrhages and leukomalacia in adults after head trauma should raise a high suspicion for a COL4A1 genetic disorder. Radiographic patterns of significant leukoaraiosis and deep microhemorrhages can also be seen in patients with long-standing vasculopathy associated with hypertension, which our patient lacked. Our findings demonstrate the utility of genetic screening for COL4A1 mutations in young patients who have small vessel vasculopathy on brain imaging but who do not have significant cardiovascular risk factors.

LT COL F.F. PIENAAR'S BOER WAR DIARY

African Journals Online (AJOL)

LT COL F.F. PIENAAR'S BOER WAR DIARY. (PART II). Brig J.H. Picard, SM*. FREE STATE TO ..... bing up and down on his master's spare horse. ... Jantjie and his master turned out of the road, and ..... on a list which burghers could afford to.

CHROMagar COL-APSE: a selective bacterial culture medium for the isolation and differentiation of colistin-resistant Gram-negative pathogens

DEFF Research Database (Denmark)

Abdul Momin, Muhd Haziq F; Bean, David C; Hendriksen, Rene S.

2017-01-01

Purpose. A selective chromogenic culture medium for the laboratory isolation and differentiation of colistin resistant Acinetobacter, Pseudomonas, Stenotrophomonas and Enterobacteriaceae spp. (CHROMagar COL-APSE) was developed, evaluated and compared to an existing selective bacterial culture......-resistant non-fermentative bacteria (Acinetobacter, Pseudomonas and Stenotrophomonas). CHROMagar COL-APSE was also more sensitive in supporting the growth of Enterobacteriaceae with COL resistance associated with the carriage of mcr-1. Conclusion. CHROMagar COL-APSE is a sensitive and specific medium...

Severe Hemolytic Jaundice in a Neonate with a Novel COL4A1 Mutation.

Science.gov (United States)

Tomotaki, Seiichi; Mizumoto, Hiroshi; Hamabata, Takayuki; Kumakura, Akira; Shiota, Mitsutaka; Arai, Hiroshi; Haginoya, Kazuhiro; Hata, Daisuke

2016-12-01

We report our experience with a preterm infant with severe hemolytic jaundice who required exchange transfusion just after birth. The patient was negative for alloimmune hemolysis as a result of maternal-fetal blood type incompatibility, and tests for inherited defects in erythrocyte metabolism, membrane function, and hemoglobin synthesis were normal. We also performed a bone marrow examination, but could not identify the cause of hemolysis. The patient had several other complications, including porencephaly, epilepsy, elevated serum levels of creatine kinase, and persistent microscopic hematuria. Later, we detected a genetic mutation in COL4A1, which was recently found to be associated with hemolytic anemia. We therefore believe that all of the patient's clinical features, including hemolytic anemia, were due to the mutation in COL4A1. Genetic testing for COL4A1 mutations is recommended in neonates who exhibit hemolytic disease of unknown etiology, especially when other complications compatible with COL4A1-related disorders are present. Copyright © 2014. Published by Elsevier B.V.

COL-3, a chemically modified tetracycline, inhibits lipopolysaccharide-induced microglia activation and cytokine expression in the brain.

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Rawan Abdulhameed Edan

Full Text Available Microglia activation results in release of proinflammatory molecules including cytokines, which contribute to neuronal damage in the central nervous system (CNS if not controlled. Tetracycline antibiotics such as minocycline inhibit microglial activation and cytokine expression during CNS inflammation. In the present study we found that administration of chemically modified tetracycline-3 (COL-3, inhibits lipopolysaccharide (LPS-induced microglial and p38 MAPK activation, as well as the increase in TNF-α, but not IL-1β expression, in the brains of BALB/c mice. COL-3 has been described to have no antibacterial activity. We observed that COL-3 had no activity against a Gram-negative bacteria, Escherichia coli; however surprisingly, COL-3 had antibacterial activity against a Gram-positive bacteria Staphylococcus aureus, with a minimum inhibitory concentration of 1 mg/ml. Our data show that COL-3 has some antibacterial activity against S. aureus, inhibits LPS-induced neuroinflammation, and displays potential as a therapeutic agent for treatment of conditions involving CNS inflammation.

Evolution of Regions Containing Antibiotic Resistance Genes in FII-2-FIB-1 ColV-Colla Virulence Plasmids.

Science.gov (United States)

Moran, Robert A; Hall, Ruth M

2018-05-01

Three ColV virulence plasmids carrying antibiotic resistance genes were assembled from draft genome sequences of commensal ST95, ST131, and ST2705 Escherichia coli isolates from healthy Australians. Plasmids pCERC4, pCERC5, and pCERC9 include almost identical backbones containing FII-2 and FIB-1 replicons and the conserved ColV virulence region with an additional ColIa determinant. Only pCERC5 includes a complete, uninterrupted F-like transfer region and was able to conjugate. pCERC5 and pCERC9 contain Tn1721, carrying the tet(A) tetracycline resistance determinant in the same location, with Tn2 (bla TEM ; ampicillin resistance) interrupting the Tn1721 in pCERC5. pCERC4 has a Tn1721/Tn21 hybrid transposon carrying dfrA5 (trimethoprim resistance) and sul1 (sulfamethoxazole resistance) in a class 1 integron. Four FII-2:FIB-1 ColV-ColIa plasmids in the GenBank nucleotide database have a related transposon in the same position, but an IS26 has reshaped the resistance gene region, deleting 2,069 bp of the integron 3'-CS, including sul1, and serving as a target for IS26 translocatable units containing bla TEM , sul2 and strAB (streptomycin resistance), or aphA1 (kanamycin/neomycin resistance). Another ColV-ColIa plasmid containing a related resistance gene region has lost the FII replicon and acquired a unique transfer region via recombination within the resistance region and at oriT. Eighteen further complete ColV plasmid sequences in GenBank contained FIB-1, but the FII replicons were of three types, FII-24, FII-18, and a variant of FII-36.

Bone mineral properties in growing Col1a2(+/G610C) mice, an animal model of osteogenesis imperfecta.

Science.gov (United States)

Masci, Marco; Wang, Min; Imbert, Laurianne; Barnes, Aileen M; Spevak, Lyudmila; Lukashova, Lyudmila; Huang, Yihe; Ma, Yan; Marini, Joan C; Jacobsen, Christina M; Warman, Matthew L; Boskey, Adele L

2016-06-01

The Col1a2(+/G610C) knock-in mouse, models osteogenesis imperfecta in a large old order Amish family (OOA) with type IV OI, caused by a G-to-T transversion at nucleotide 2098, which alters the gly-610 codon in the triple-helical domain of the α2(I) chain of type I collagen. Mineral and matrix properties of the long bones and vertebrae of male Col1a2(+/G610C) and their wild-type controls (Col1a2(+/+)), were characterized to gain insight into the role of α2-chain collagen mutations in mineralization. Additionally, we examined the rescuability of the composition by sclerostin inhibition initiated by crossing Col1a2(+/G610C) with an LRP(+/A214V) high bone mass allele. At age 10-days, vertebrae and tibia showed few alterations by micro-CT or Fourier transform infrared imaging (FTIRI). At 2-months-of-age, Col1a2(+/G610C) tibias had 13% fewer secondary trabeculae than Col1a2(+/+), these were thinner (11%) and more widely spaced (20%) than those of Col1a2(+/+) mice. Vertebrae of Col1a2(+/G610C) mice at 2-months also had lower bone volume fraction (38%), trabecular number (13%), thickness (13%) and connectivity density (32%) compared to Col1(a2+/+). The cortical bone of Col1a2(+/G610C) tibias at 2-months had 3% higher tissue mineral density compared to Col1a2(+/+); Col1a2(+/G610C) vertebrae had lower cortical thickness (29%), bone area (37%) and polar moment of inertia (38%) relative to Col1a2(+/+). FTIRI analysis, which provides information on bone chemical composition at ~7μm-spatial resolution, showed tibias at 10-days did not differ between genotypes. Comparing identical bone types in Col1a2(+/G610C) to Col1a2(+/+) at 2-months-of-age, tibias showed higher mineral-to-matrix ratio in trabeculae (17%) and cortices (31%). and in vertebral cortices (28%). Collagen maturity was 42% higher at 10-days-of-age in Col1a2(+/G610C) vertebral trabeculae and in 2-month tibial cortices (12%), vertebral trabeculae (42%) and vertebral cortices (12%). Higher acid-phosphate substitution

RNase H and replication of ColE1 DNA in Escherichia coli.

OpenAIRE

Naito, S; Uchida, H

1986-01-01

Amber mutations within the rnh (RNase H) gene of Escherichia coli K-12 were isolated by selecting for bacteria capable of replicating in a sup+ background replication-defective cer-6 mutant of the ColE1 replicon. The cer-6 mutation is an alteration of one base pair located 160 nucleotides upstream of the unique replication origin of this plasmid. Subsequently, we determined the DNA alterations present within these mutants. ColE1 DNA replicated in rnh(Am) recA cells, indicating that (i) RNase ...

Fetal origin of brain damage in 2 infants with a COL4A1 mutation: fetal and neonatal MRI

NARCIS (Netherlands)

Vermeulen, R. J.; Peeters-Scholte, C.; van Vugt, J. J. M.; van Vught, J. J. M. G.; Barkhof, F.; Rizzu, P.; van der Schoor, S. R. D.; van der Knaap, M. S.

2011-01-01

Mutations in the gene COL4A1, encoding collagen IV A1, are associated with familial porencephaly. Previously, COL4A1 mutation-associated antenatal hemorrhages have been suggested by early post-natal imaging. We describe 2 children with fetal intracerebral hemorrhages and a COL4A1 mutation. There was

Variação do volume de gotas de colírios lubrificantes disponíveis no mercado brasileiro

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Vitorugo Silvestre Nascimento

Full Text Available RESUMO Objetivo: Avaliar a variação intra e interexaminadores do volume de gotas dispensados de frascos de colírios lubrificantes disponíveis no mercado. Métodos: Foram estudados cinco frascos de colírios lubrificantes e dezenove voluntários participaram deste estudo. A massa média de gotas de 20µl dos colírios foi obtida utilizando micropipeta e balança de precisão e como padrão para comparação com a massa das gotas obtidas pelos voluntários. Cinco gotas de cada frasco foram pesadas individualmente com o tubo de colírio perpendicular à balança, usando o primeiro e segundo dedos da mão direita, de forma que a pressão fosse aplicada somente no meio do frasco. Os experimentos foram realizados em uma sala climatizada a temperatura ambiente (21±1°C. Resultados: Todos os frascos de colírios apresentaram variação estatisticamente significante das massas das gotas obtidas pelos examinadores quando comparadas com a massa média padrão de 0,0182±0,0014g, com exceção da comparação entre os dados do colírio A com o colírio D, que não apresentou variação estatisticamente significante. Conclusão: O presente estudo demonstra a ausência de uniformidade das gotas dispensadas pelos frascos de colírios disponíveis no mercado e a sua inadequação à real necessidade, uma vez que as gotas dispensadas são maiores do que o indicado. Esse fato torna-se um problema quando se trata de período de tratamento prolongado, especialmente com colírios dispendiosos como os indicados para a terapêutica do glaucoma. Nesse sentido, a padronização das gotas de colírios se faz necessária.

Avaliação do custo de colírios lubrificantes a base exclusivamente de carboximetilcelulose no mercado brasileiro

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Paulo Estacia

2013-10-01

Full Text Available OBJETIVO: Avaliar o custo do uso dos colírios lubrificantes mais utilizados no mercado nacional que apresentam na formulação o mesmo princípio ativo - carboximetilcelulose, considerando o número total de gotas presente em cada frasco dos colírios. MÉTODOS: Foi realizado um estudo experimental utilizando três frascos de cada um dos colírios Lacrifilm® (colírio 1 e Fresh Tears® (colírio 2. Para análise do custo dos colírios contou-se o número de gotas de cada frasco correlacionando os preços dos mesmos. O preço considerado para cada medicação foi o mínimo ao consumidor com alíquota de 17% publicado para o mês de janeiro de 2012. A análise estatística foi efetuada em SPSS® 18. A comparação das variáveis quantitativas analisadas foi procedida através do teste não paramétrico Mann-Whitney e correlação linear de Spearman, sendo considerada uma diferença estatisticamente significante um valor de probabilidade inferior a 0,05. RESULTADOS: Verificou-se que há diferença estatisticamente significativa entre as marcas de colírios avaliados com relação ao número total de gotas. O colírio 1 apresentou o maior número de gotas. CONCLUSÃO: O colírio 1, que tem o maior número de gotas por frasco, também é o que tem o preço mais acessível. Portanto, verifica-se que é o produto mais econômico, ou seja, o paciente pagará menos por cada gota.

Volume da gota dos colírios lubrificantes: estudo farmacoeconômico

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Alexandre Xavier da Costa

2015-12-01

Full Text Available RESUMO Objetivo: Determinar o volume médio das gotas produzidas pelos colírios lubrificantes em diferentes ângulos de inclinação e determinar o custo médio do tratamento. Métodos: Determinação do volume da gota de 3 frascos originais dos colírios lubrificantes Artelac®, Hylo Comod®, Lacrima® Plus, Systane® UL, Lacrifilm®, Hyabak®, Lacribell®, Ecofilm®, Mirugell®, Plenigell®, Fresh Tears®, Optive® e Endura® à inclinação de 90º e 45º. Determinou-se o número médio de gotas em cada frasco e foi feita avaliação farmacoeconômica dos colírios. Resultados: O volume das gotas variou de 32,2 a 64,0 µL a 45o e de 29,1 a 65,1 µL a 90o. A diferença entre as gotas em cada inclinação foi de 2 a 24% e o custo anual dos colírios de acordo com a inclinação variou de R$2,73 a R$130,73. A Duração Máxima de Tratamento (DMT foi de 29,3 a 51,4 dias na inclinação de 45o, e de 28,8 a 48,4 dias a 90º, sendo que a diferença na DMT foi de 0,5 até 8 dias a mais ou a menos, de acordo com a marca. Conclusão: Nenhum dos colírios estudados apresentou gotas ideais para o olho humano, levando a um desperdício do produto e maior custo para o fabricante e para o consumidor. Percebemos que existe uma variação significativa no volume da gota de acordo com a inclinação do frasco, e que uma variação maior do que 10% traria impactos financeiros para o paciente.

A Gene Gun-mediated Nonviral RNA trans-splicing Strategy for Col7a1 Repair

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Patricia Peking

2016-01-01

Full Text Available RNA trans-splicing represents an auspicious option for the correction of genetic mutations at RNA level. Mutations within COL7A1 causing strong reduction or absence of type VII collagen are associated with the severe skin blistering disease dystrophic epidermolysis bullosa. The human COL7A1 mRNA constitutes a suitable target for this RNA therapy approach, as only a portion of the almost 9 kb transcript has to be delivered into the target cells. Here, we have proven the feasibility of 5′ trans-splicing into the Col7a1 mRNA in vitro and in vivo. We designed a 5′ RNA trans-splicing molecule, capable of replacing Col7a1 exons 1–15 and verified it in a fluorescence-based trans-splicing model system. Specific and efficient Col7a1 trans-splicing was confirmed in murine keratinocytes. To analyze trans-splicing in vivo, we used gene gun delivery of a minicircle expressing a FLAG-tagged 5′ RNA trans-splicing molecule into the skin of wild-type mice. Histological and immunofluorescence analysis of bombarded skin sections revealed vector delivery and expression within dermis and epidermis. Furthermore, we have detected trans-spliced type VII collagen protein using FLAG-tag antibodies. In conclusion, we describe a novel in vivo nonviral RNA therapy approach to restore type VII collagen expression for causative treatment of dystrophic epidermolysis bullosa.

Linkage mapping of the gene for Type III collagen (COL3A1) to human chromosome 2q using a VNTR polymorphism

Energy Technology Data Exchange (ETDEWEB)

Tiller, G.E.; Polumbo, P.A.; Summar, M.L. (Vanderbilt Univ. Medical Center, Nashville, TN (United States))

1994-03-15

The gene for the [alpha]1(III) chain of type III collagen, COL3A1, has been previously mapped to human chromosome 2q24.3-q31 by in situ hybridization. Physical mapping by pulsed-field gel electrophoresis has demonstrated that COL3A1 lies within 35 kb of COL5A2. The authors genotyped the CEPH families at the COL3A2 locus using a pentanucleotide repeat polymorphism within intron 25. They demonstrated significant linkage to 18 anonymous markers as well as the gene for carbamyl phosphate synthetase (CPSI), which had been previously mapped to this region. No recombination was seen between COL3A1 and COL5A2 (Z = 9.93 at [theta] = 0) or D2S24 (Z = 10.55 at [theta] = 0). The locus order is (D2S32-D2S138-D2S148)-(D2S24-COL5A2-COL3A1)-(D2S118-D2S161), with odds of 1:2300 for the next most likely order. These relationships are consistent with the physical mapping of COL3A1 to the distal portion of 2q and place it proximal to CPSI by means of multipoint analysis. These linkage relationships should prove useful in further studies of Ehlers-Danlos syndrome type IV and carbamyl phosphate synthetase I deficiency and provide an additional framework for localizing other genes in this region. 13 refs., 2 figs., 1 tab.

Novel COL4A1 mutations associated with HANAC syndrome: a role for the triple helical CB3[IV] domain.

Science.gov (United States)

Plaisier, Emmanuelle; Chen, Zhiyong; Gekeler, Florian; Benhassine, Safa; Dahan, Karine; Marro, Béatrice; Alamowitch, Sonia; Paques, Michel; Ronco, Pierre

2010-10-01

The COL4A1 gene encodes the α1-chain of type IV collagen, which is ubiquitously expressed in basement membranes. Mutations in COL4A1 have been reported in autosomal-dominant porencephaly and in patients with symptomatic small vessel brain disease, inconstantly associated with eye defects. We have previously reported three COL4A1 mutations associated with a systemic phenotype that we called HANAC (Hereditary Angiopathy, Nephropathy, Aneurysms, and Cramps). We carried out a clinical and genetic study of three families presenting with characteristic features of HANAC syndrome. Common systemic signs included arterial retinal tortuosity and muscle cramps, with a variable combination of small vessel brain disease, Raynaud phenomena, and kidney defects. Three novel COL4A1 missense substitutions are described, which affect highly conserved glycine residues within the collagenous domain of the protein. All six known mutations associated with the HANAC phenotype are localized within the CB3[IV] fragment of COL4A1, which encompasses major integrin-binding sites. Our results confirm that HANAC syndrome is a distinct clinical entity within the COL4A1-related disorders, which is characterized by systemic involvement and usually asymptomatic brain disease. The restricted distribution of COL4A1 mutations within the CB3[IV] region is a characteristic of the reports of patients with HANAC, which suggests that abnormal cell-type IV collagen interactions may underlie the systemic defects observed in this syndrome. Copyright © 2010 Wiley-Liss, Inc.

Tissue-specific mosaicism for a lethal osteogenesis imperfecta COL1A1 mutation causes mild OI/EDS overlap syndrome.

Science.gov (United States)

Symoens, Sofie; Steyaert, Wouter; Demuynck, Lynn; De Paepe, Anne; Diderich, Karin E M; Malfait, Fransiska; Coucke, Paul J

2017-04-01

Type I collagen is the predominant protein of connective tissues such as skin and bone. Mutations in the type I collagen genes (COL1A1 and COL1A2) mainly cause osteogenesis imperfecta (OI). We describe a patient with clinical signs of Ehlers-Danlos syndrome (EDS), including fragile skin, easy bruising, recurrent luxations, and fractures resembling mild OI. Biochemical collagen analysis of the patients' dermal fibroblasts showed faint overmodification of the type I collagen bands, a finding specific for structural defects in type I collagen. Bidirectional Sanger sequencing detected an in-frame deletion in exon 44 of COL1A1 (c.3150_3158del), resulting in the deletion of three amino acids (p.Ala1053_Gly1055del) in the collagen triple helix. This COL1A1 mutation was hitherto identified in four probands with lethal OI, and never in EDS patients. As the peaks on the electropherogram corresponding to the mutant allele were decreased in intensity, we performed next generation sequencing of COL1A1 to study mosaicism in skin and blood. While approximately 9% of the reads originating from fibroblast gDNA harbored the COL1A1 deletion, the deletion was not detected in gDNA from blood. Most likely, the mild clinical symptoms observed in our patient can be explained by the mosaic state of the mutation. © 2017 Wiley Periodicals, Inc.

Effects of a malfunctional column on conventional and FeedCol-simulated moving bed chromatography performance.

Science.gov (United States)

Song, Ji-Yeon; Oh, Donghoon; Lee, Chang-Ha

2015-07-17

The effects of a malfunctional column on the performance of a simulated moving bed (SMB) process were studied experimentally and theoretically. The experimental results of conventional four-zone SMB (2-2-2-2 configuration) and FeedCol operation (2-2-2-2 configuration with one feed column) with one malfunctional column were compared with simulation results of the corresponding SMB processes with a normal column configuration. The malfunctional column in SMB processes significantly deteriorated raffinate purity. However, the extract purity was equivalent or slightly improved compared with the corresponding normal SMB operation because the complete separation zone of the malfunctional column moved to a lower flow rate range in zones II and III. With the malfunctional column configuration, FeedCol operation gave better experimental performance (up to 7%) than conventional SMB operation because controlling product purity with FeedCol operation was more flexible through the use of two additional operating variables, injection time and injection length. Thus, compared with conventional SMB separation, extract with equivalent or slightly better purity could be produced from FeedCol operation even with a malfunctional column, while minimizing the decrease in raffinate purity (less than 2%). Copyright © 2015 Elsevier B.V. All rights reserved.

A novel COL11A1 missense mutation in siblings with non-ocular Stickler syndrome.

Science.gov (United States)

Kohmoto, Tomohiro; Tsuji, Atsumi; Morita, Kei-Ichi; Naruto, Takuya; Masuda, Kiyoshi; Kashimada, Kenichi; Enomoto, Keisuke; Morio, Tomohiro; Harada, Hiroyuki; Imoto, Issei

2016-01-01

Stickler syndrome (STL) is an autosomal, dominantly inherited, clinically variable and genetically heterogeneous connective tissue disorder characterized by ocular, auditory, orofacial and skeletal abnormalities. We conducted targeted resequencing using a next-generation sequencer for molecular diagnosis of a 2-year-old girl who was clinically suspected of having STL with Pierre Robin sequence. We detected a novel heterozygous missense mutation, NM_001854.3:n.4838G>A [NM_001854.3 (COL11A1_v001):c.4520G>A], in COL11A1, resulting in a Gly to Asp substitution at position 1507 [NM_001854.3(COL11A1_i001)] within one of the collagen-like domains of the triple helical region. The same mutation was detected in her 4-year-old brother with cleft palate and high-frequency sensorineural hearing loss.

EuroCirCol - Horizon 2020 Research and Innovation Action

CERN Document Server

AUTHOR|(CDS)2108815; Benedikt, Michael; Zimmermann, Frank; Schulte, Daniel; Tommasini, Davide; Seryi, Andrei; Perez, Francis

2015-01-01

EuroCirCol is a conceptual design study for a post-LHC research infrastructure based on an energy-frontier 100 TeV circular hadron collider. ​The award of the 2013 Nobel Prize for Physics acknowledged the leading role of Europe in particle physics, which has a global community of over 10'000 scientists. To reinforce its pole position throughout the 21st century, Europe must be ready to propose an ambitious post-LHC accelerator project by 2018/19. This is one of the main recommendations of the updated European Strategy for Particle Physics, adopted by the CERN Council in May 2013. The EuroCirCol conceptual design study is a direct response to this recommendation, carried out through a collaboration of institutes and universities worldwide. A new research infrastructure of such scale depends on the feasibility of key technologies pushed beyond current state of the art. Innovative designs for accelerator magnets to achieve high-quality fields up to 16 T and for a cryogenic beam vacuum system to cope with unpre...

Evaluation of a patient with classical Ehlers-Danlos syndrome due to a 9q34 duplication affecting COL5A1.

Science.gov (United States)

Kuroda, Yukiko; Ohashi, Ikuko; Naruto, Takuya; Ida, Kazumi; Enomoto, Yumi; Saito, Toshiyuki; Nagai, Jun-Ichi; Kurosawa, Kenji

2018-03-09

Ehlers-Danlos syndrome classical type is a connective tissue disorder characterized by skin hyperextensibility, atrophic scarring, and joint hypermobility. The condition typically results from mutations in COL5A1 or COL5A2 leading to the functional haploinsufficiency. Here, we report of a 24-year-old male with mild intellectual disability, dysmorphic features, and a phenotype consistent with Ehlers-Danlos syndrome classical type. A copy number variant-calling algorithm from panel sequencing data identified the deletions exons 2-11 and duplications of exons 12-67 within COL5A1. Array comparative genomic hybridization confirmed a 94 kb deletion at 9q34.3 involving exons 2-11 of COL5A1, and a 3.4 Mb duplication at 9q34.3 involving exons 12-67 of COL5A1. © 2018 Japanese Teratology Society.

78 FR 15053 - Simpson Lumber Company, LLC, Shelton, Washington; Simpson Lumber Company, LLC, Tacoma, Washington...

Science.gov (United States)

2013-03-08

...,372B] Simpson Lumber Company, LLC, Shelton, Washington; Simpson Lumber Company, LLC, Tacoma, Washington; Simpson Lumber Company, LLC, Longview, Washington; Notice of Revised Determination on Reconsideration On... Reconsideration for the workers and former workers of Simpson Lumber Company, LLC, Shelton, Washington (TA-W-81...

Loss of Col3a1, the gene for Ehlers-Danlos syndrome type IV, results in neocortical dyslamination.

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Sung-Jin Jeong

Full Text Available It has recently been discovered that Collagen III, the encoded protein of the type IV Ehlers-Danlos Syndrome (EDS gene, is one of the major constituents of the pial basement membrane (BM and serves as the ligand for GPR56. Mutations in GPR56 cause a severe human brain malformation called bilateral frontoparietal polymicrogyria, in which neurons transmigrate through the BM causing severe mental retardation and frequent seizures. To further characterize the brain phenotype of Col3a1 knockout mice, we performed a detailed histological analysis. We observed a cobblestone-like cortical malformation, with BM breakdown and marginal zone heterotopias in Col3a1⁻/⁻ mouse brains. Surprisingly, the pial BM appeared intact at early stages of development but starting as early as embryonic day (E 11.5, prominent BM defects were observed and accompanied by neuronal overmigration. Although collagen III is expressed in meningeal fibroblasts (MFs, Col3a1⁻/⁻ MFs present no obvious defects. Furthermore, the expression and posttranslational modification of α-dystroglycan was undisturbed in Col3a1⁻/⁻ mice. Based on the previous finding that mutations in COL3A1 cause type IV EDS, our study indicates a possible common pathological pathway linking connective tissue diseases and brain malformations.

Pertinencia de los postgrados para economistas en el Núcleo LUZ–COL

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Ana Teresa Prieto Sánchez

2002-01-01

Full Text Available El presente artículo tiene como finalidad presentar los resultados obtenidos en un estudio de mercado, cuyo objetivo fue determinar la pertinencia de los postgrados para economistas en el Núcleo LUZ-COL. En dicho estudio se analizaron las expectativas, necesidades y percepciones de los usuarios potenciales del servicio de postgrado, considerando como usuarios potenciales (demanda a los entes empleadores de la COL, docentes de economía en el Núcleo, egresados del programa de economía en el Núcleo y estudiantes del último semestre de la carrera. Estos elementos sirvieron de base para conocer la pertinencia o no del postgrado. La metodología utilizada se fundamentó en una investigación descriptiva y cualitativa, en donde se utilizó el cuestionario como instrumento, para recabar la información primaria. Los resultados muestran una alta necesidad de los programas de postgrados para economistas y delimitan una demanda potencial considerable. El análisis permitió concluir que sí son pertinentes los postgrados para economistas en el Núcleo COL, en las áreas de gerencia de empresas, formulación y evaluación de proyectos, costos o finanzas; del tipo maestría o especialización profesional. En virtud de los resultados, se recomienda, desarrollar estrategias viables que permitan a los tomadores de decisión implementar el postgrado para economistas en el Núcleo.

El rol del professor en l’aprenentatge col·laboratiu mediat per ordinador

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Ingrid Noguera-Fructuoso

2009-10-01

Full Text Available En aquest article es presenten els resultats d'una investigació sobre el canvi de rol que es produeix en el professorat en l'ús de les TIC com a eina per a l'aprenentatge col·laboratiu en educació primària i secundària. L'interès es focalitza en l'ús concret d'una eina dissenyada per a l'aprenentatge col·laboratiu, el BSCL, sota el model pedagògic de qüestionament progressiu. En primer lloc, es duu a terme una revisió teòrica. En segon lloc, s'exposen les preguntes d'investigació, la justificació d'aquesta, es descriu el disseny i l'enfocament metodològic escollit, l'estudi de casos. Els resultats obtinguts revelen que hi ha una tendència cap al canvi de rol en el professorat en l'ús del BSCL com a eina per a l'aprenentatge col·laboratiu així com l'eficàcia del model de qüestionament progressiu en la construcció de coneixement de l'alumnat.

Complete COL1A1 allele deletions in osteogenesis imperfecta

NARCIS (Netherlands)

van Dijk, Fleur S.; Huizer, Margriet; Kariminejad, Ariana; Marcelis, Carlo L.; Plomp, Astrid S.; Terhal, Paulien A.; Meijers-Heijboer, Hanne; Weiss, Marjan M.; van Rijn, Rick R.; Cobben, Jan M.; Pals, Gerard

Purpose: To identify a molecular genetic cause in patients with a clinical diagnosis of osteogenesis imperfecta (OI) type I/IV. Methods: The authors performed multiplex ligation-dependent probe amplification analysis of the COL1A1 gene in a group of 106 index patients. Results: In four families with

Complete COL1A1 allele deletions in osteogenesis imperfecta

NARCIS (Netherlands)

van Dijk, Fleur S.; Huizer, Margriet; Kariminejad, Ariana; Marcelis, Carlo L.; Plomp, Astrid S.; Terhal, Paulien A.; Meijers-Heijboer, Hanne; Weiss, Marjan M.; van Rijn, Rick R.; Cobben, Jan M.; Pals, Gerard

2010-01-01

To identify a molecular genetic cause in patients with a clinical diagnosis of osteogenesis imperfecta (OI) type I/IV. The authors performed multiplex ligation-dependent probe amplification analysis of the COL1A1 gene in a group of 106 index patients. In four families with mild osteogenesis

Cisto gigante de colédoco Giant choledochal cyst

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Olival Cirilo Lucena da Fonseca-Neto

2007-12-01

Full Text Available INTRODUÇÃO: A doença cística das vias biliares é anomalia congênita que pode acometer as vias biliares intra e/ou extra-hepáticas. A extra-hepática inclui os cistos de colédoco e a intra-hepática é conhecida por Doença de Caroli. Os cistos de colédoco de tamanho gigante são muito raros. OBJETIVO: Relatar o caso de um cisto de colédoco de tamanho gigante em uma paciente feminina. RELATO DE CASO: Mulher de 19 anos foi admitida com história de icterícia e acolia fecal há sete dias. Referia dor epigástrica associada com ingestão de dieta rica em gordura. Nos antecedentes pessoais relatou dois episódios de icterícia, aos 8 e 14 anos, que progrediram espontaneamente. No exame físico apresentava icterícia (+3/+4 e uma massa palpável indolor em mesogástrio foram os únicos achados. A ultrassonografia demonstrou grande formação cística de paredes finas adjacente ao hepatocolédoco, pâncreas e rim direito que media 18,5 x 10,2 cm. A colangioressonância confirmou o grande cisto de colédoco e hepatojejunoanastomose em "Y" de Roux após excisão do cisto e colecistectomia foi realizada. A formação cística media 20 x 15,5 x 12,5 cm e com um volume médio de 1000 mL. A paciente encontra-se em acompanhamento ambulatorial sem alterações hepatobiliares após o sétimo mês da operação. CONCLUSÃO: O cisto de colédoco deve fazer parte do diagnóstico diferencial em pacientes adultos jovens com icterícia e massa palpável; no entanto, a diferenciação entre ele e neoplasia maligna deve ser pesquisada.BACKGROUND: Choledochal cyst represents a rare congenital anomaly, eventually associated with intra and extrahepatic biliary tract disorders. Extrahepatic diseases include choledochal cysts and congenital dilation of the lower intrahepatic bile duct is known as Caroli's disease. Giant choledochal cyst constitutes a very rare abnormality. AIM:To report a giant choledochal cyst in a female patient. CASE REPORT: A 19-year

Genetics Home Reference: COL4A1-related brain small-vessel disease

Science.gov (United States)

... hemorrhage Johns Hopkins Medicine Department of Neurology and Neurosurgery: Intracerebral Hemorrhage Johns Hopkins Medicine Department of Neurology and Neurosurgery: Stroke MalaCards: col4a1-related brain small-vessel disease ...

Stimulation of Escherichia coli F-18Col- Type-1 fimbriae synthesis by leuX

DEFF Research Database (Denmark)

Newman, Joseph V.; Burghoff, Robert L.; Pallesen, Lars

1994-01-01

Escherichia coli F-18, a normal human fecal isolate, is an excellent colonizer of the streptomycin-treated mouse large intestine. E. coli F-18Col-, a derivative of E. coli F-18 which no longer makes the E. coli F-18 colicin, colonizes the large intestine as well as E. coli F-18 when fed to mice...... alone but is eliminated when fed together with E. coli F-18. Recently we randomly cloned E. coli F-18 DNA into E. coli F-18Col- and let the mouse intestine select the best colonizer. In this way, we isolated a 6.5-kb E. coli F-18 DNA sequence that simultaneously stimulated synthesis of type 1 fimbriae...... and enhanced E. coli F-18Col- colonizing ability. In the present investigation we show that the gene responsible for stimulation of type 1 fimbriae synthesis appears to be leuX, which encodes a tRNA specific for the rare leucine codon UUG. Moreover, it appears that expression of leuX may be regulated by two...

Wound healing genes and susceptibility to cutaneous leishmaniasis in Brazil: Role of COL1A1

Science.gov (United States)

Almeida, Lucas; Oliveira, Joyce; Guimarães, Luiz Henrique; Carvalho, Edgar M; Blackwell, Jenefer M

2015-01-01

Previous studies have demonstrated a role for wound healing genes in resolution of cutaneous lesions caused by Leishmania spp. in both mice and humans, including the gene FLI1 encoding Friend leukaemia virus integration 1. Reduction of Fli1 expression in mice has been shown to result in up-regulation of collagen type I alpha 1 (Col1a1) and alpha 2 (Col1a2) genes and, conversely, in down-regulation of the matrix metalloproteinase 1 (Mmp1) gene, suggesting that Fli1 suppression is involved in activation of the profibrotic gene program. Here we examined single nucleotide polymorphisms (SNPs) in these genes as risk factors for cutaneous (CL) and mucosal leishmaniasis (ML), and leishmaniasis per se, caused by L. braziliensis in humans. SNPs were genotyped in 168 nuclear families (250 CL; 87 ML cases) and replicated in 157 families (402 CL; 39 ML cases). Family-based association tests (FBAT) showed the strongest association between SNPs rs1061237 (combined P=0.002) and rs2586488 (combined P=0.027) at COL1A1 and CL disease. This contributes to our further understanding of the role of wound healing in the resolution of CL disease, providing potential for therapies modulating COL1A1 via drugs acting on FLI1. PMID:25562121

PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene Capture Platforms

OpenAIRE

Manuel Ares-Arroyo; Cristina Bernabe-Balas; Alfonso Santos-Lopez; Maria R. Baquero; Kashi N. Prasad; Dolores Cid; Carmen Martin-Espada; Alvaro San Millan; Bruno Gonzalez-Zorn

2018-01-01

ColE1 plasmids are important vehicles for the spread of antibiotic resistance in the Enterobacteriaceae and Pasteurellaceae families of bacteria. Their monitoring is essential, as they harbor important resistant determinants in humans, animals and the environment. In this work, we have analyzed ColE1 replicons using bioinformatic and experimental approaches. First, we carried out a computational study examining the structure of different ColE1 plasmids deposited in databases. Bioinformatic an...

Receptores do factor estimulante de colónias de macrófagos do robalo

OpenAIRE

Oliveira, Márcio Adriano Guiomar de

2010-01-01

O receptor do factor estimulante de colónias de macrófagos, também conhecido como receptor do factor estimulante de colónias-1 (CSF1R), é um receptor de um factor de crescimento hematopoiético que é especificamente expresso em células do sistema fagocítico-mononuclear e desempenha um papel essencial no desenvolvimento e regulação destas células. O CSF1R já foi descrito em vários mamíferos e a sua biologia tem sido exaustivamente caracterizada nestes vertebrados mas o conheci...

PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene Capture Platforms

Directory of Open Access Journals (Sweden)

Manuel Ares-Arroyo

2018-03-01

Full Text Available ColE1 plasmids are important vehicles for the spread of antibiotic resistance in the Enterobacteriaceae and Pasteurellaceae families of bacteria. Their monitoring is essential, as they harbor important resistant determinants in humans, animals and the environment. In this work, we have analyzed ColE1 replicons using bioinformatic and experimental approaches. First, we carried out a computational study examining the structure of different ColE1 plasmids deposited in databases. Bioinformatic analysis of these ColE1 replicons revealed a mosaic genetic structure consisting of a host-adapted conserved region responsible for the housekeeping functions of the plasmid, and a variable region encoding a wide variety of genes, including multiple antibiotic resistance determinants. From this exhaustive computational analysis we developed a new PCR-based technique, targeting a specific sequence in the conserved region, for the screening, capture and sequencing of these small plasmids, either specific for Enterobacteriaceae or specific for Pasteurellaceae. To validate this PCR-based system, we tested various collections of isolates from both bacterial families, finding that ColE1 replicons were not only highly prevalent in antibiotic-resistant isolates, but also present in susceptible bacteria. In Pasteurellaceae, ColE1 plasmids carried almost exclusively antibiotic resistance genes. In Enterobacteriaceae, these plasmids encoded a large range of traits, including not only antibiotic resistance determinants, but also a wide variety of genes, showing the huge genetic plasticity of these small replicons. Finally, we also used a metagenomic approach in order to validate this technique, performing this PCR system using total DNA extractions from fecal samples from poultry, turkeys, pigs and humans. Using Illumina sequencing of the PCR products we identified a great diversity of genes encoded by ColE1 replicons, including different antibiotic resistance

Identification of a novel COL1A1 frameshift mutation, c.700delG, in a Chinese osteogenesis imperfecta family

Science.gov (United States)

Wang, Xiran; Pei, Yu; Dou, Jingtao; Lu, Juming; Li, Jian; Lv, Zhaohui

2015-01-01

Osteogenesis imperfecta (OI) is a family of genetic disorders associated with bone loss and fragility. Mutations associated with OI have been found in genes encoding the type I collagen chains. People with OI type I often produce insufficient α1-chain type I collagen because of frameshift, nonsense, or splice site mutations in COL1A1 or COL1A2. This report is of a Chinese daughter and mother who had both experienced two bone fractures. Because skeletal fragility is predominantly inherited, we focused on identifying mutations in COL1A1 and COL1A2 genes. A novel mutation in COL1A1, c.700delG, was detected by genomic DNA sequencing in the mother and daughter, but not in their relatives. The identification of this mutation led to the conclusion that they were affected by mild OI type I. Open reading frame analysis indicated that this frameshift mutation would truncate α1-chain type I collagen at residue p263 (p.E234KfsX264), while the wild-type protein would contain 1,464 residues. The clinical data were consistent with the patients’ diagnosis of mild OI type I caused by haploinsufficiency of α1-chain type I collagen. Combined with previous reports, identification of the novel mutation COL1A1-c.700delG in these patients suggests that additional genetic and environmental factors may influence the severity of OI. PMID:25983617

A PTEN-COL17A1 fusion gene and its novel regulatory role in Collagen XVII expression and GBM malignance.

Science.gov (United States)

Yan, Xiaoyan; Zhang, Chuanbao; Liang, Tingyu; Yang, Fan; Wang, Haoyuan; Wu, Fan; Wang, Wen; Wang, Zheng; Cheng, Wen; Xu, Jiangnan; Jiang, Tao; Chen, Jing; Ding, Yaozhong

2017-10-17

Collagen XVII expression has recently been demonstrated to be correlated with the tumor malignance. While Collagen XVII is known to be widely distributed in neurons of the human brain, its precise role in pathogenesis of glioblastoma multiforme (GBM) is unknown. In this study, we identified and characterized a new PTEN-COL17A1 fusion gene in GMB using transcriptome sequencing. Although fusion gene did not result in measurable fusion protein production, its presence is accompanied with high levels of COL17A1 expression, revealed a novel regulatory mechanism of Collagen XVII expression by PTEN-COL17A1 gene fusion. Knocked down Collagen XVII expression in glioma cell lines resulted in decreased tumor invasiveness, along with significant reduction of MMP9 expression, while increased Collagen XVII expression promotes invasive activities of glioma cells and associated with GBM recurrences. Together, our results uncovered a new PTEN-COL17A1 fusion gene and its novel regulatory role in Collagen XVII expression and GBM malignance, and demonstrated that COL17A1 could serve as a useful prognostic biomarker and therapeutic targets for GBM.

Generation of Col2a1-EGFP iPS cells for monitoring chondrogenic differentiation.

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Taku Saito

Full Text Available Induced pluripotent stem cells (iPSC are a promising cell source for cartilage regenerative medicine; however, the methods for chondrocyte induction from iPSC are currently developing and not yet sufficient for clinical application. Here, we report the establishment of a fluorescent indicator system for monitoring chondrogenic differentiation from iPSC to simplify screening for effective factors that induce chondrocytes from iPSC. We generated iPSC from embryonic fibroblasts of Col2a1-EGFP transgenic mice by retroviral transduction of Oct4, Sox2, Klf4, and c-Myc. Among the 30 clones of Col2a1-EGFP iPSC we established, two clones showed high expression levels of embryonic stem cell (ESC marker genes, similar to control ESC. A teratoma formation assay showed that the two clones were pluripotent and differentiated into cell types from all three germ layers. The fluorescent signal was observed during chondrogenic differentiation of the two clones concomitant with the increase in chondrocyte marker expression. In conclusion, Col2a1-EGFP iPSC are useful for monitoring chondrogenic differentiation and will contribute to research in cartilage regenerative medicine.

12 CFR 4.4 - Washington office.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Washington office. 4.4 Section 4.4 Banks and... EXAMINERS Organization and Functions § 4.4 Washington office. The Washington office of the OCC is the main office and headquarters of the OCC. The Washington office directs OCC policy, oversees OCC operations...

Historical changes to Lake Washington and route of the Lake Washington Ship Canal, King County, Washington

Science.gov (United States)

Chrzastowski, Michael J.

1983-01-01

Lake Washington, in the midst of the greater Seattle metropolitan area of the Puget Sound region (fig. 1), is an exceptional commercial, recreational, and esthetic resource for the region . In the past 130 years, Lake Washington has been changed from a " wild " lake in a wilderness setting to a regulated lake surrounded by a growing metropolis--a transformation that provides an unusual opportunity to study changes to a lake's shoreline and hydrologic characteristics -resulting from urbanization.

EuroCirCol kick-off event

CERN Multimedia

Hardre, Julie

2015-01-01

The EuroCirCol (http://cern.ch/eurocircol) kick-off event at CERN on June 2-4 brought together 62 participants to constitute governance bodies, commit to the project plan and align the organisation, structures and processes of 16 institutions from 10 countries. The goal of the project is to conceive a post-LHC research infrastructure around a 100 km circular energy-frontier hadron collider capable of reaching 100 TeV collisions. The project officially started on June 1 and will run for four years. The total estimated budget of 11.2 million Euros includes a 2.99 million Euro contribution from the Horizon 2020 programme on developing new world-class research infrastructures (http://cordis.europa.eu/project/rcn/194962_en.html).

ROCK inhibition stimulates SOX9/Smad3-dependent COL2A1 expression in inner meniscus cells.

Science.gov (United States)

Furumatsu, Takayuki; Maehara, Ami; Ozaki, Toshifumi

2016-07-01

Proper functioning of the meniscus depends on the composition and organization of its fibrocartilaginous extracellular matrix. We previously demonstrated that the avascular inner meniscus has a more chondrocytic phenotype compared with the outer meniscus. Inhibition of the Rho family GTPase ROCK, the major regulator of the actin cytoskeleton, stimulates the chondrogenic transcription factor Sry-type HMG box (SOX) 9-dependent α1(II) collagen (COL2A1) expression in inner meniscus cells. However, the crosstalk between ROCK inhibition, SOX9, and other transcription modulators on COL2A1 upregulation remains unclear in meniscus cells. The aim of this study was to investigate the role of SOX9-related transcriptional complex on COL2A1 expression under the inhibition of ROCK in human meniscus cells. Human inner and outer meniscus cells were prepared from macroscopically intact lateral menisci. Cells were cultured in the presence or absence of ROCK inhibitor (ROCKi, Y27632). Gene expression, collagen synthesis, and nuclear translocation of SOX9 and Smad2/3 were analyzed. Treatment of ROCKi increased the ratio of type I/II collagen double positive cells derived from the inner meniscus. In real-time PCR analyses, expression of SOX9 and COL2A1 genes was stimulated by ROCKi treatment in inner meniscus cells. ROCKi treatment also induced nuclear translocation of SOX9 and phosphorylated Smad2/3 in immunohistological analyses. Complex formation between SOX9 and Smad3 was increased by ROCKi treatment in inner meniscus cells. Chromatin immunoprecipitation analyses revealed that association between SOX9/Smad3 transcriptional complex with the COL2A1 enhancer region was increased by ROCKi treatment. This study demonstrated that ROCK inhibition stimulated SOX9/Smad3-dependent COL2A1 expression through the immediate nuclear translocation of Smad3 in inner meniscus cells. Our results suggest that ROCK inhibition can stimulates type II collagen synthesis through the cooperative activation

A novel missense mutation of COL5A2 in a patient with Ehlers-Danlos syndrome.

Science.gov (United States)

Watanabe, Miki; Nakagawa, Ryuji; Naruto, Takuya; Kohmoto, Tomohiro; Suga, Ken-Ichi; Goji, Aya; Kagami, Shoji; Masuda, Kiyoshi; Imoto, Issei

2016-01-01

Ehlers-Danlos syndrome (EDS) is a group of inherited connective tissue disorders characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. For molecular diagnosis, targeted exome sequencing was performed on a 9-year-old male patient who was clinically suspected to have EDS. The patient presented with progressive kyphoscoliosis, joint hypermobility and hyperextensible skin without scars. Ultimately, classical EDS was diagnosed by identifying a novel, mono-allelic mutation in COL5A2 [NM_000393.3(COL5A2_v001):c.682G>A, p.Gly228Arg].

A novel missense mutation of COL5A2 in a patient with Ehlers–Danlos syndrome

Science.gov (United States)

Watanabe, Miki; Nakagawa, Ryuji; Naruto, Takuya; Kohmoto, Tomohiro; Suga, Ken-ichi; Goji, Aya; Kagami, Shoji; Masuda, Kiyoshi; Imoto, Issei

2016-01-01

Ehlers–Danlos syndrome (EDS) is a group of inherited connective tissue disorders characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. For molecular diagnosis, targeted exome sequencing was performed on a 9-year-old male patient who was clinically suspected to have EDS. The patient presented with progressive kyphoscoliosis, joint hypermobility and hyperextensible skin without scars. Ultimately, classical EDS was diagnosed by identifying a novel, mono-allelic mutation in COL5A2 [NM_000393.3(COL5A2_v001):c.682G>A, p.Gly228Arg]. PMID:27656288

ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice

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Frances E. Jones

2016-02-01

Full Text Available Collagen IV is a major component of basement membranes, and mutations in COL4A1, which encodes collagen IV alpha chain 1, cause a multisystemic disease encompassing cerebrovascular, eye and kidney defects. However, COL4A1 renal disease remains poorly characterized and its pathomolecular mechanisms are unknown. We show that Col4a1 mutations in mice cause hypotension and renal disease, including proteinuria and defects in Bowman's capsule and the glomerular basement membrane, indicating a role for Col4a1 in glomerular filtration. Impaired sodium reabsorption in the loop of Henle and distal nephron despite elevated aldosterone levels indicates that tubular defects contribute to the hypotension, highlighting a novel role for the basement membrane in vascular homeostasis by modulation of the tubular response to aldosterone. Col4a1 mutations also cause diabetes insipidus, whereby the tubular defects lead to polyuria associated with medullary atrophy and a subsequent reduction in the ability to upregulate aquaporin 2 and concentrate urine. Moreover, haematuria, haemorrhage and vascular basement membrane defects confirm an important vascular component. Interestingly, although structural and compositional basement membrane defects occurred in the glomerulus and Bowman's capsule, no tubular basement membrane defects were detected. By contrast, medullary atrophy was associated with chronic ER stress, providing evidence for cell-type-dependent molecular mechanisms of Col4a1 mutations. These data show that both basement membrane defects and ER stress contribute to Col4a1 renal disease, which has important implications for the development of treatment strategies for collagenopathies.

Colômbia 2010: Guerra, Paz e Eleições

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Luiz Antônio Gusmão

2009-02-01

Full Text Available

O enfraquecimento das Farc deve polarizar as

eleições de 2010 na Colômbia entre recrudescimento

do combate e abertura à negociação.

Adição de colágeno em pão sem glúten elaborado com farinha de arroz

Directory of Open Access Journals (Sweden)

Carolina da Silva Graça

2017-02-01

Full Text Available Resumo Os portadores da doença celíaca, uma enteropatia, apresentam intolerância ao glúten e a restrição de alimentos que o contenham mostra-se eficaz na normalização da função intestinal e das manifestações clínicas. O objetivo do presente estudo foi avaliar o efeito da adição de colágeno em pó e em fibra nas características estruturais, tecnológicas e sensoriais de pães sem glúten, elaborados com farinha de arroz. Primeiramente, foram realizadas adições de 2%, 4%, 6% e 8% de colágeno, sob as formas em pó e em fibra, em pães elaborados com farinha de arroz, sendo estes avaliados quanto às suas características tecnológicas: volume específico, firmeza do miolo e cor da crosta e do miolo. O pão que apresentou as melhores características tecnológicas foi caracterizado quanto à sua composição química (proteínas, lipídios, cinzas, umidade e carboidratos, avaliado quanto às perdas decorrentes da cocção e submetido à análise sensorial. As massas cruas dos pães adicionados com o colágeno em fibra, assim como a massa do pão padrão (sem adição de colágeno, foram avaliadas através de reologia e microscopia eletrônica de varredura. A adição de colágeno aos pães elaborados com farinha de arroz não colaborou para a formação de uma rede semelhante à formada pelo glúten. O pão da formulação com 4% de colágeno em fibra apresentou as características tecnológicas mais próximas ao esperado, não diferindo significativamente quanto à composição química, do pão padrão. Por meio da análise sensorial, constatou-se que os pães sem glúten (padrão e com 4% de colágeno em fibra obtiveram aceitação sensorial superior a 75%. Conclui-se, com o estudo, que é possível adicionar colágeno em pães sem glúten, sendo que o pão adicionado de 4% de colágeno em fibra apresentou características mais próximas às do pão padrão testado.

Better Jobs, Brighter Futures, a Stronger Washington. Washington's Community and Technical Colleges

Science.gov (United States)

Washington State Board for Community and Technical Colleges, 2015

2015-01-01

The world is changing rapidly. With changes in technology, demographics, and workforce trends, Washington needs colleges to not only keep pace, but lead the way. Washington's 34 community and technical colleges answer that call. The community and technical colleges have proven uniquely positioned to adapt to, embrace, and ignite change. Community…

Variability and distribution of COL1A2 (type I collagen) polymorphisms in the central-eastern Mediterranean Basin.

Science.gov (United States)

Scorrano, Gabriele; Lelli, Roberta; Martínez-Labarga, Cristina; Scano, Giuseppina; Contini, Irene; Hafez, Hani S; Rudan, Pavao; Rickards, Olga

2016-01-01

The most abundant of the collagen protein family, type I collagen is encoded by the COL1A2 gene. The COL1A2 restriction fragment length polymorphisms (RFLPs) EcoRI, RsaI and MspI in samples from several different central-eastern Mediterranean populations were analysed and found to be potentially informative anthropogenetic markers. The objective was to define the genetic variability of COL1A2 in the central-eastern Mediterranean and to shed light on its genetic distribution in human groups over a wide geographic area. PCR-RFLP analysis of EcoRI, RsaI and MspI polymorphisms of the COL1A2 gene was performed on oral swab and blood samples from 308 individuals from the central-eastern Mediterranean Basin. The genetic similarities among these groups and other populations described in the literature were investigated through correspondence analysis. Single-marker data and haplotype frequencies seemed to suggest a genetic homogeneity within the European populations, whereas a certain degree of differentiation was noted for the Egyptians and the Turks. The genetic variability in the central-eastern Mediterranean area is probably a result of the geographical barrier of the Mediterranean Sea, which separated European and African populations over time.

Oil spill response issues in Washington State

International Nuclear Information System (INIS)

Lempriere, P.R.

1997-01-01

Washington State statutes and regulations applicable to oil transport and oil spills were described. Specific provisions of the statutes and regulations and other relevant matters were also discussed. Among these were: (1) Washington State oil spill prevention plans, (2) Washington State oil spill contingency plans, (3) best achievable protection, (4) Intertanko's lawsuit against Washington State, (5) oil spill removal organizations, (6) certificates of financial responsibility in Washington State, (7) extent of potential liability under Washington Law, (8) disposal of cleanup materials, and (9) definition of 'qualified individuals' on marine vessels having the authority to implement removal actions

Expressão do colágeno I em carcinomas epidermóides da cavidade oral

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Martins Gabriela Botelho

2003-01-01

Full Text Available A matriz extracelular no carcinoma epidermóide da cavidade oral sofre modificações qualitativas e quantitativas durante a sua progressão. Estas alterações parecem possibilitar os processos de invasão e metástase que caracterizam as neoplasias malignas e são mais evidentes na interface tumor-hospedeiro. No nosso estudo, 23 biópsias incisionais de carcinomas epidermóides de diversas localizações da cavidade oral foram classificadas segundo o sistema de graduação histológica preconizado por Anneroth et al.¹ (1987. Foram utilizadas as colorações pela H. E. e a do tipo picrosirius, específica para colágeno. A expressão imuno-histoquímica do colágeno tipo I foi analisada em lesões com diferentes escores histológicos de malignidade. Pôde-se concluir que o colágeno apresenta diferentes padrões morfológicos, histoquímicos e imuno-histoquímicos em lesões com altos e baixos escores de malignidade.

Linkage analysis in a family with Stickler syndrome leads to the exclusion of the COL2A1 locus

Energy Technology Data Exchange (ETDEWEB)

Mottes, M.; Zolezzi, F.; Pignatti, P.F. [Univ. of Verona (Italy)

1994-09-01

Hereditary arthro-ophtalmopathy (AO) or Stickler Syndrome (MIM No. 10830) is a dominantly inherited disorder characterized by vitro-retinal degeneration and other connective tissue disturbances. Mutations in the COL2A1 gene, coding for type II collagen chains, have been described in a few patients. The wide spectrum of clinical manifestations is presumably due to genetic heterogeneity, since only about 50% of the Stickler families so far studied show cosegregation of the disease with the COL2A1 locus. We have investigated a large pedigree (19 individuals of whom 9 are affected) in which severe myopia with vitro-retinal degeneration consegregated with joint laxity, recurrent inguinal hernias, and degenerative changes of the hip and the knee. The 3{prime} end COL2A1 VNTR polymorphism was utilized for linkage analysis. In order to get the maximum informativity, we have analyzed the allelic microheterogeneity of this VNTR, due to the repeat sequence variation, by means of a single strand polymorphism. Mendelian inheritance of the different single strands was observed as expected. Discordance of segregation between the disease and the COL2A1 locus was thus established inequivocally in this family.

Support of positive association in family-based genetic analysis between COL27A1 and Tourette syndrome.

Science.gov (United States)

Liu, Shiguo; Yu, Xiaoxia; Xu, Quanchen; Cui, Jiajia; Yi, Mingji; Zhang, Xinhua; Ge, Yinlin; Ma, Xu

2015-08-03

Recently, a genome-wide association study has indicated associations between single nucleotide polymorphisms in the Collagen Type XXVII Alpha 1 gene (COL27A1) and Tourette syndrome in several ethnic populations. To clarify the global relevance of the previously identified SNPs in the development of Tourette syndrome, the associations between polymorphisms in COL27A1 and Tourette syndrome were assessed in Chinese trios. PCR-directed sequencing was used to evaluate the genetic contributions of three SNPs in COL27A1(rs4979356, rs4979357 and rs7868992) using haplotype relative risk (HRR) and transmission disequilibrium tests (TDT) with a total of 260 Tourette syndrome trios. The family-based association was significant between Tourette syndrome and rs4979356 (TDT: χ2 = 4.804, P = 0.033; HRR = 1.75, P = 0.002; HHRR = 1.32, P = 0.027), and transmission disequilibrium was suspected for rs4979357 (TDT: χ2 = 3.969, P = 0.053; HRR = 1.84, P = 0.001; HHRR = 1.29, P = 0.044). No statistically significant allele transfer was found for rs7868992 (TDT: χ2 = 2.177, P = 0.158). Although the TDT results did not remain significant after applying the conservative Bonferroni correction (p = 0.005), the significant positive HRR analysis confirmed the possibility of showing transmission disequilibrium, which provides evidence for an involvement of COL27A1in the development of TS. However, these results need to be verified with larger datasets from different populations.

Molecular Evolution and Phylogenetic Analysis of Eight COL Superfamily Genes in Group I Related to Photoperiodic Regulation of Flowering Time in Wild and Domesticated Cotton (Gossypium) Species

Science.gov (United States)

Zhang, Rui; Ding, Jian; Liu, Chunxiao; Cai, Caiping; Zhou, Baoliang; Zhang, Tianzhen; Guo, Wangzhen

2015-01-01

Flowering time is an important ecological trait that determines the transition from vegetative to reproductive growth. Flowering time in cotton is controlled by short-day photoperiods, with strict photoperiod sensitivity. As the CO-FT (CONSTANS-FLOWER LOCUS T) module regulates photoperiodic flowering in several plants, we selected eight CONSTANS genes (COL) in group I to detect their expression patterns in long-day and short-day conditions. Further, we individually cloned and sequenced their homologs from 25 different cotton accessions and one outgroup. Finally, we studied their structures, phylogenetic relationship, and molecular evolution in both coding region and three characteristic domains. All the eight COLs in group I show diurnal expression. In the orthologous and homeologous loci, each gene structure in different cotton species is highly conserved, while length variation has occurred due to insertions/deletions in intron and/or exon regions. Six genes, COL2 to COL5, COL7 and COL8, exhibit higher nucleotide diversity in the D-subgenome than in the A-subgenome. The Ks values of 98.37% in all allotetraploid cotton species examined were higher in the A-D and At-Dt comparison than in the A-At and D-Dt comparisons, and the Pearson’s correlation coefficient (r) of Ks between A vs. D and At vs. Dt also showed positive, high correlations, with a correlation coefficient of at least 0.797. The nucleotide polymorphism in wild species is significantly higher compared to G. hirsutum and G. barbadense, indicating a genetic bottleneck associated with the domesticated cotton species. Three characteristic domains in eight COLs exhibit different evolutionary rates, with the CCT domain highly conserved, while the B-box and Var domain much more variable in allotetraploid species. Taken together, COL1, COL2 and COL8 endured greater selective pressures during the domestication process. The study improves our understanding of the domestication-related genes/traits during cotton

Targeted deletion of RANKL in M cell inducer cells by the Col6a1-Cre driver.

Science.gov (United States)

Nagashima, Kazuki; Sawa, Shinichiro; Nitta, Takeshi; Prados, Alejandro; Koliaraki, Vasiliki; Kollias, George; Nakashima, Tomoki; Takayanagi, Hiroshi

2017-11-04

The gut-associated lymphoid tissues (GALTs), including Peyer's patches (PPs), cryptopatches (CPs) and isolated lymphoid follicles (ILFs), establish a host-microbe symbiosis by the promotion of immune reactions against gut microbes. Microfold cell inducer (MCi) cells in GALTs are the recently identified mesenchymal cells that express the cytokine RANKL and initiate bacteria-specific immunoglobulin A (IgA) production via induction of microfold (M) cell differentiation. In the previous study, the Twist2-Cre driver was utilized for gene deletion in mesenchymal cells including MCi cells. In order to investigate MCi cells more extensively, it will be necessary to develop experimental tools in addition to the Twist2-Cre driver mice and characterize such drivers in specificity and efficiency. Here we show that M cell differentiation and IgA production are impaired in the targeted deletion of RANKL by the Col6a1-Cre driver. We compared Col6a1-Cre with Twist2-Cre in terms of the specificity for mesenchymal cells in GALTs. Col6a1-Cre CAG-CAT-EGFP mice exhibited EGFP expression in podoplanin + CD31 - cells including MCi cells, while Twist2-Cre mice were shown to target endothelial cells and podoplanin + CD31 - cells. Tnfsf11 fl/Δ Col6a1-Cre mice exhibited the absence of M cells and severe IgA reduction together with an alteration in gut microbial composition. Moreover, we analyzed germ free mice to test whether changes in the microbiota are the cause of M cell deficiency. M cell differentiation was normal in the CPs/ILFs of germ free mice, indicating that MCi cells induce M cells independently of microbial colonization. This study demonstrates that Col6a1-Cre driver mice are as useful as Twist2-Cre driver mice for functional analyses of GALT-resident mesenchymal cells, including MCi cells. Copyright © 2017 Elsevier Inc. All rights reserved.

Columbian Secrets: Cristóbal Colón by Leonardo Balada and Antonio Gala

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José Elías Gutiérrez Meza

2016-11-01

Full Text Available The play Cristóbal Colón premiered in 1989 at the Teatro del Liceu. Composed by the musician Leonardo Balada and the playwright Antonio Gala, this opera was commissioned by the Sociedad Estatal Quinto Centenario as part of the festivities organized by the Spanish government to commemorate the Discovery of America. Unlike the so-called "des-celebración del 92", Cristóbal Colón champions the figure of the discoverer and emphasizes the participation of Spain through the praised role of Martín Alonso Pinzón. However, the opera was cut at the last minute. This article asserts that such alteration may have been the result of political censorship attempting to counteract the criticism of the Discovery of America contained in the original version.

Produção de identidades nos trabalhos apresentados em colóquios luso-brasileiros de 2002 e 2004

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Leonardo Dias Ferreira

2017-12-01

Full Text Available O presente artigo trata-se do estudo da internacionalização do campo do currículo com enfoque em trabalhos apresentados em colóquios luso-brasileiros de 2002 e 2004 sobre questões curriculares e produção de identidades. Para isso, faremos uma contextualização histórica do colóquio luso-brasileiro, depois refletiremos sobre a internacionalização do campo do currículo e a visão desta concepção presente nos editoriais dos colóquios Luso-Brasileiros e na última seção falaremos sobre a categoria identidade. Concluiu-se que  entender a relação direta de questões sobre multiculturalidade e formação da identidade do sujeito envolvido no processo de ensino-aprendizagem escolar é essencial para que a escola seja voltada para formação e interação social, de tal modo, que os alunos possam compartilhar suas crenças e valores e aprender a respeitar, reconhecer e valorizar as diferenças do outro. Palavras Chaves: internacionalização do campo do currículo; colóquios luso-brasileiros, identidade.

Projecting Politics: The Grapes of Wrath Les Raisins de la colère : Une critique politique

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Stephen J. Whitfield

2008-01-01

Full Text Available Publié en 1939 et adapté à l’écran l’année suivante, Les Raisins de la colère, roman de John Steinbeck, eut un impact immédiat et extraordinaire pour le portrait sans concession qu’il dressait d’un capitalisme américain qui semblait avoir abandonné sa progéniture la plus loyale, la plus assidue et la plus courageuse, celle qui labourait la terre en plein cœur de la république. Écrit à la toute fin de la « Décennie rouge », mais juste avant que les États-Unis entrent dans la Seconde Guerre mondiale, Les Raisins de la colère exprimaient, d’une certaine manière, la colère accumulée contre un système économique qui avait trahi le prolétariat rural. Le roman tout comme le film de John Ford (1940 furent compris par le grand public de l’époque, et bien des années plus tard, comme une protestation clairement gauchiste, voire radicale, contre une telle injustice. Mais cet essai montre combien les idées politiques de ces œuvres jumelées sont en réalité difficiles à saisir et combien il est hasardeux de définir, quel que soit l’effort rétrospectif, la critique que Steinbeck et Ford ont proposée dans leur version des Raisins de la colère, une œuvre inoubliable, déchirante mais ambiguë sur le plan politique.

The impact of ColRS two-component system and TtgABC efflux pump on phenol tolerance of Pseudomonas putida becomes evident only in growing bacteria

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Kivisaar Maia

2010-04-01

Full Text Available Abstract Background We have recently found that Pseudomonas putida deficient in ColRS two-component system is sensitive to phenol and displays a serious defect on solid glucose medium where subpopulation of bacteria lyses. The latter phenotype is significantly enhanced by the presence of phenol in growth medium. Here, we focused on identification of factors affecting phenol tolerance of the colR-deficient P. putida. Results By using transposon mutagenesis approach we identified a set of phenol-tolerant derivatives of colR-deficient strain. Surprisingly, half of independent phenol tolerant clones possessed miniTn5 insertion in the ttgABC operon. However, though inactivation of TtgABC efflux pump significantly enhanced phenol tolerance, it did not affect phenol-enhanced autolysis of the colR mutant on glucose medium indicating that phenol- and glucose-caused stresses experienced by the colR-deficient P. putida are not coupled. Inactivation of TtgABC pump significantly increased the phenol tolerance of the wild-type P. putida as well. Comparison of phenol tolerance of growing versus starving bacteria revealed that both ColRS and TtgABC systems affect phenol tolerance only under growth conditions and not under starvation. Flow cytometry analysis showed that phenol strongly inhibited cell division and to some extent also caused cell membrane permeabilization to propidium iodide. Single cell analysis of populations of the ttgC- and colRttgC-deficient strains revealed that their membrane permeabilization by phenol resembles that of the wild-type and the colR mutant, respectively. However, cell division of P. putida with inactivated TtgABC pump seemed to be less sensitive to phenol than that of the parental strain. At the same time, cell division appeared to be more inhibited in the colR-mutant strain than in the wild-type P. putida. Conclusions ColRS signal system and TtgABC efflux pump are involved in the phenol tolerance of P. putida. However, as

A experiência com arte na Colônia Juliano Moreira na década de 1950

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João Henrique Queiroz de Araújo

Full Text Available Resumo Os trabalhos de Osório César e de Nise da Silveira costumam se destacar quando se investiga a relação entre expressão artística e saberes psi no Brasil em torno da década de 1950. Porém, outras experiências ocorreram no mesmo período, como as atividades de pintura realizadas na Seção de Praxiterapia da Colônia Juliano Moreira, em Jacarepaguá, no município do Rio de Janeiro. A ausência de pesquisas sobre essa experiência suscitou uma busca por documentos que culminou na análise dos números do Boletim da Colônia Juliano Moreira e demais fontes. Os resultados apontam características peculiares da experiência artística na colônia, como o incentivo à cópia e reprodução de fotografias como método terapêutico, o que a diferencia das outras experiências mencionadas.

Population haplotype analysis and evolutionary relations of the COL2A1 gene

NARCIS (Netherlands)

Meulenbelt, I.; Williams, G.J.; Koppele, J.M.T.E.; Giessen, G.C.D.E. van; Slagboom, P.E.

1996-01-01

We have determined the allele frequencies mad pairwise linkage disequilibria of restriction fragment length polymorphisms (RFLPs) distributed over the entire COL2A1 gene (spanning 23 · 6 kb) in a population of unrelated Dutch Caucasians. Pairwise linkage disequilibrium analysis of RFLP sites between

Las cuentas del cuarto viaje de Cristóbal Colón

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Gil, Juan

2003-12-01

Full Text Available The payroll of the fourth voyage of Christopher Columbus is here published for the fisrt time, signed by Admiral himself. A commentary is also furnished.

Se publican por primera vez, con comentario, las cuentas del cuarto viaje de Cristóbal Colón, firmadas por el propio almirante.

Avaliação da preferência alimentar em modelo de poliartrite induzida por colágeno

OpenAIRE

Eduarda Correa Freitas

2014-01-01

Introdução: Artrite reumatoide é uma doença inflamatória crônica associada a alterações no metabolismo proteico e energético. Objetivos: Investigar a preferência alimentar durante o desenvolvimento da inflamação crônica em ratas com artrite induzida por colágeno. Métodos: Ratas Wistar fêmeas foram alocadas em dois grupos: controle (CO, n=10) e artrite induzida por colágeno (CIA; n=11) e expostas simultaneamente a quatro diferentes dietas (padrão, hipercalórica, hiperlipidica e hiperproteica)....

Variables físicas, químicas y biológicas del suelo sobre las poblaciones de colémbolos en Costa Rica

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Cesar Guillen

2006-01-01

Full Text Available Se ha discutido que las variables físicas, químicas y biológicas del suelo influyen sobre las poblaciones de colémbolos; sin embargo, la información acerca de la interacción de estas variables, en conjunto, con relación a la diversidad y abundancia de colémbolos, es escasa. En el presente estudio se determinó la relación entre estas variables y la diversidad y abundancia de colémbolos en los suelos de un bosque primario, un bosque secundario y un cafetal en el Parque Nacional Tapantí. El bosque primario presentó los contenidos más altos de materia orgánica y biomasa microbiana, contrario a lo sucedido con la resistencia a la penetración y el pH. Los resultados mostraron una clara asociación entre estas variables y algunas especies de colémbolos, lo cual indica que los cambios en la estructura de las comunidades de colémbolos, por efecto de los cambios en las variables físicas, químicas y biológicas del suelo, pueden ser utilizados como un bioindicador de calidad del suelo y de manejo de los ecosistemas.

Collagen Type I alpha1 (COL1A1 Gene Polymorphism and Bone Mineral Density in Postmenopausal Kazakh Women

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Akbota Aitkulova

2014-12-01

Full Text Available Introduction: Single nucleotide polymorphism (SNP at the collagen type I alpha 1 gene (COL1A1 rs1800012 has been widely studied and has shown an association with bone mineral density (BMD and fractures. A minor allele TT of this SNP was found to be greatly overrepresented in individuals with fractures compared to controls, thus becoming a good predictor of  increased fracture risk. The aim of this investigation was to evaluate potential association between COL1A1 gene polymorphism and osteoporosis in Kazakh postmenopausal women.Methods: The study population included 103 postmenopausal women recruited from Pavlodar and Almaty clinics. BMD was measured using DEXA. Genomic DNA was extracted from peripheral venous blood of study participants with Wizard® Genomic DNA Purification Kit (Promega, USA. Detection of COL1A1 +1245G/T (Sp1 polymorphism was done by the TaqMan® SNP Genotyping Assay of real-time PCR.Results: Densitometry results revealed 36 osteoporotic, 42 osteopenic, and 25 normal postmenopausal women. Data analysis of 1245G>T polymorphism in COL1A1 gene in the group of women with osteopenia and osteoporosis revealed deviation from Hardy-Weinberg equilibrium. The mutant TT genotype was prevalent compared to the heterozygous genotype GT in both groups. Distributions were 83% GG, 3% GT, and 14% TT in the group with osteopenia and 80% GG, 6% GT, and 14% TT in the group with osteoporosis. The distribution of genotypes frequency in the group of normal postmenopausal women was 76% GG, 16% GT, and 8% TT.Conclusion: These results suggest that TT genotype of COL1A1 +1245G/T (Sp1 polymorphism is associated with risk of postmenopausal osteoporosis in Kazakh women. Further studies involving a larger number of women are needed to clarify the relationship of this polymorphism with risk of osteoporosis. 

El almirante Cristóbal Colón y la ciudad de Valladolid

OpenAIRE

Varela Marcos , Jesús

2006-01-01

Pretendemos aunar todas las presencias de Colón en la ciudad de Valladolid en el año de la conmemoración de su muerte. Para ello hemos rastreado en la documentación todos los momentos en que el genovés estuvo en dicha villa.

Different NaCl-Induced Calcium Signatures in the Arabidopsis thaliana Ecotypes Col-0 and C24

KAUST Repository

Schmöckel, Sandra M.

2015-02-27

A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.

Different NaCl-Induced Calcium Signatures in the Arabidopsis thaliana Ecotypes Col-0 and C24

KAUST Repository

Schmö ckel, Sandra M.; Garcia, Alexandre F.; Berger, Bettina; Tester, Mark A.; Webb, Alex A. R.; Roy, Stuart J.

2015-01-01

A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.

Toxicidade córneo-conjuntival do colírio de iodo-povidona: estudo experimental

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Santos Namir Clementino

2003-01-01

Full Text Available OBJETIVO: Avaliar a toxicidade ocular do colírio de iodo-povidona a 2,5% e a 0,5% sobre a superfície ocular, na regeneração do epitélio corneal e as alterações histopatológicas da córnea. MÉTODOS: Realizaram-se estudos experimentais consecutivos em coelhos albinos, nos quais se fez a ablação do epitélio de uma área circular central da córnea de 6,5 mm de diâmetro. Em cada experimento foram utilizados 20 animais (40 olhos, sendo que no olho direito foi instilado o colírio de iodo-povidona (caso e no olho esquerdo água destilada (controle, em intervalos de uma hora, durante três dias consecutivos. Durante o experimento, os animais foram submetidos a exames biomicroscópicos diários para avaliação da superfície córneo-conjuntival e realização de fotografias seriadas da área sem epitélio, corada com fluoresceína, para medida da área projetada da lesão com auxílio de analisador de imagem computadorizado. No final do experimento, os animais foram sacrificados para avaliação histopatológica das córneas. RESULTADOS: O colírio de iodo-povidona a 2,5% comprometeu a regeneração epitelial, causou conjuntivite em 100% dos olhos, com produção de secreção de aspecto mucoso em 80%, ceratite ponteada em 40% e edema estrômico leve em 10% dos casos. Os achados histopatológicos foram úlcera de córnea, degeneração hidrópica das células endoteliais e infiltrado inflamatório com predomínio de eosinófilos em 100% dos casos. Nos olhos em que se instilou iodo-povidona a 0,5%, assim como nos controles, observou-se completa regeneração da lesão epitelial (p<0,001 após 72 horas do início do experimento. Do ponto de vista histopatológico, epitelização normal em todos os casos e controles, em apenas um caso observou-se discreto infiltrado de leucócitos perilímbicos. CONCLUSÃO: A toxicidade ocular do colírio de iodo-povidona é dependente da concentração da solução, sendo que o colírio a 2,5% mostrou

Extensive Analysis of GmFTL and GmCOL Expression in Northern Soybean Cultivars in Field Conditions.

Science.gov (United States)

Guo, Guangyu; Xu, Kun; Zhang, Xiaomei; Zhu, Jinlong; Lu, Mingyang; Chen, Fulu; Liu, Linpo; Xi, Zhang-Ying; Bachmair, Andreas; Chen, Qingshan; Fu, Yong-Fu

2015-01-01

The FLOWERING LOCUS T (FT) gene is a highly conserved florigen gene among flowering plants. Soybean genome encodes six homologs of FT, which display flowering activity in Arabidopsis thaliana. However, their contributions to flowering time in different soybean cultivars, especially in field conditions, are unclear. We employed six soybean cultivars with different maturities to extensively investigate expression patterns of GmFTLs (Glycine max FT-like) and GmCOLs (Glycine max CO-like) in the field conditions. The results show that GmFTL3 is an FT homolog with the highest transcript abundance in soybean, but other GmFTLs may also contribute to flower induction with different extents, because they have more or less similar expression patterns in developmental-, leaf-, and circadian-specific modes. And four GmCOL genes (GmCOL1/2/5/13) may confer to the expression of GmFTL genes. Artificial manipulation of GmFTL expression by transgenic strategy (overexpression and RNAi) results in a distinct change in soybean flowering time, indicating that GmFTLs not only impact on the control of flowering time, but have potential applications in the manipulation of photoperiodic adaptation in soybean. Additionally, transgenic plants show that GmFTLs play a role in formation of the first flowers and in vegetative growth.

The Variants Within the COL5A1 Gene are Associated with Reduced Risk of Anterior Cruciate Ligament Injury in Skiers

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Stępień-Słodkowska Marta

2015-03-01

Full Text Available The purpose of this study was to examine the association of the BstUI RFLP C/T (rs 12722 and DpnII RFLP C/T (rs 13946 COL5A1 polymorphisms, individually and as haplotypes, with anterior cruciate ligament ruptures in recreational skiers. Subjects were 138 male recreational skiers with surgically diagnosed primary anterior cruciate ligament ruptures. The control group consisted of 183 apparently healthy male recreational skiers, who were without any self-reported history of ligament or tendon injury. DNA was extracted from buccal cells donated by the subjects and genotyping was carried out using real-time PCR. The genotype distributions for both polymorphisms met Hardy- Weinberg expectations in both groups. There were no significant differences in genotype distribution of allele frequencies of COL5A1 BstUI RFLP C/T and COL5A1 DpnII RFLP C/T polymorphisms between the ACL rupture and control groups. The T-T (BstUI RFLP T, DpnII RFLP T haplotype was the most common (55.6%. The haplotype T-C was not present in any of the subjects. There was an underrepresentation tendency of the C-T haplotype in the study group compared to controls under recessive mode of inheritance. Higher frequency of the COL5A1 BstUI RFLP C/T and COL5A1DpnII RFLP C/T polymorphisms haplotype is associated with reduced risk of anterior cruciate ligament injury in a group of apparently healthy male recreational skiers.

Projecting Politics: The Grapes of Wrath Les Raisins de la colère à l’écran

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Stephen J. Whitfield

2009-07-01

Full Text Available Publié en 1939 et adapté à l’écran l’année suivante, Les Raisins de la colère, roman de John Steinbeck, eut un impact immédiat et extraordinaire pour le portrait sans concession qu’il dressait d’un capitalisme américain qui semblait avoir abandonné sa progéniture la plus loyale, la plus assidue et la plus courageuse, celle qui labourait la terre en plein coeur de la république. Écrit à la toute fin de la « Décennie rouge », mais juste avant que les États-Unis entrent dans la Seconde Guerre mondiale, Les Raisins de la colère exprimaient, d’une certaine manière, la colère accumulée contre un système économique qui avait trahi le prolétariat rural. Le roman, tout comme le film de John Ford (1940, furent compris par le grand public de l’époque, et bien des années plus tard, comme une protestation clairement gauchiste, voire radicale, contre une telle injustice. Mais cet essai montre combien les idées politiques de ces œuvres jumelées sont en réalité difficiles à saisir et combien il est hasardeux de définir, quel que soit l’effort rétrospectif, la critique que Steinbeck et Ford ont proposée dans leur version des Raisins de la colère, une œuvre inoubliable, déchirante mais ambiguë sur le plan politique.

DICE/ColDICE: 6D collisionless phase space hydrodynamics using a lagrangian tesselation

Science.gov (United States)

Sousbie, Thierry

2018-01-01

DICE is a C++ template library designed to solve collisionless fluid dynamics in 6D phase space using massively parallel supercomputers via an hybrid OpenMP/MPI parallelization. ColDICE, based on DICE, implements a cosmological and physical VLASOV-POISSON solver for cold systems such as dark matter (CDM) dynamics.

Inquérito sobre fungos anemófilos na fronteira Brasil - Colômbia

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Simão Arão Pecher

1988-06-01

Full Text Available Foi realizado um inquérito, sobre fungos anemófllos, em três vilarejos situados na fronteira Brasil-Colômbia, zona muito quente e úmida, durante o mês de julho de 1978. Em apenas uma coleta feita, em diferentes pontos, foram cultivadas colônias pertencentes a dezoito gêneros diferentes de fungos anemófllos, com predominância de Mucor (64,3%, Cândida (54,8%, Rhodotorula (38,1% e Penicillium (38,1%.An inquiry of anemophilous fungi was carried out in small towns located at the Brasilian- Colombian border, a hot and humid zone, during the month of July, 1978. In just one collection, carried out at different sites, colonies were cultivated, and belonged to eighteen different species of anemophilous fungi with predominance of Mucor (64.3%, Candida (54.8%, Rhodotorula (38.1% and Penicillium (38.1%.

Genetic Variations of COL4A1 Gene and Intracerebral Hemorrhage Outcome: A Cohort Study in a Chinese Han Population.

Science.gov (United States)

Xia, Chao; Lin, Sen; Yang, Jie; He, Sha; Li, Hao; Liu, Ming; You, Chao

2018-05-01

To investigate the relationship between single nucleotide polymorphisms or haplotypes of COL4A1 gene and the outcome of intracerebral hemorrhage (ICH). In our study, 181 patients with hypertensive ICH were enrolled and followed up at 3 and 6 months. Outcome data included any cause of death and disability. Genomic DNA was extracted by DNA extraction kit, and the 6 single nucleotide polymorphism genotyping of the COL4A1 gene was detected through MassARRAY Analyzer. Unphased 3.1.4 and SPSS 19.0 were used to analyze the association between alleles, genotypes, and haplotypes of the COL4A1 gene and the outcomes of ICH. Of the 181 patients with hypertensive ICH, 12 were lost in follow-up, which accounted for 6.6%. Our association analysis showed that the rs532625 AA genotype of the COL4A1 gene may increase risk of disability at 3 months; the rs532625 A allele and AA genotype were association factors of the risk of disability at 6 months; the rs532625 AA genotype was an association factor of the risk of death/disability at 6 months. After adjusting for gender, age, coma, and severe neurologic deficits, only the rs532625 AA genotype was independently associated with the risk of disability at 3 and 6 months and the risk of death/disability at 6 months. Our study found that the rs532625 AA genotype in the COL4A1 gene was independently associated with the risk of disability at 3 and 6 months and death/disability at 6 months in a Chinese Han population. These conclusions need to be verified in future studies with larger samples. Copyright © 2018 Elsevier Inc. All rights reserved.

Prolonged exposure of methicillin-resistant Staphylococcus aureus (MRSA) COL strain to increasing concentrations of oxacillin results in a multidrug-resistant phenotype

DEFF Research Database (Denmark)

Martins, Ana; Couto, Isabel; Aagaard, Lone

2007-01-01

Our previous studies demonstrated that exposure of a bacterium to increasing concentrations of an antibiotic would increase resistance to that antibiotic as a consequence of activating efflux pumps. This study utilises the same approach; however, it employs the methicillin-resistant Staphylococcus...... aureus (MRSA) COL strain, which is highly resistant to oxacillin (OXA). MRSA COL was adapted to 3200 mg/L of OXA. Changes in resistance to other antibiotics were evaluated and efflux pump activity during the adaptation process was determined. MRSA COL was exposed to stepwise two-fold increases of OXA....... At the end of each step, minimum inhibitory concentration determination for erythromycin (ERY) and other antibiotics was conducted. Reserpine (RES) was employed to evaluate whether resistance to ERY was dependent on efflux pump activity. Efflux pump activity was also evaluated using the ethidium bromide (EB...

A novel splice variant in the N-propeptide of COL5A1 causes an EDS phenotype with severe kyphoscoliosis and eye involvement.

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Sofie Symoens

Full Text Available BACKGROUND: The Ehlers-Danlos Syndrome (EDS is a heritable connective tissue disorder characterized by hyperextensible skin, joint hypermobility and soft tissue fragility. The classic subtype of EDS is caused by mutations in one of the type V collagen genes (COL5A1 and COL5A2. Most mutations affect the type V collagen helical domain and lead to a diminished or structurally abnormal type V collagen protein. Remarkably, only two mutations were reported to affect the extended, highly conserved N-propeptide domain, which plays an important role in the regulation of the heterotypic collagen fibril diameter. We identified a novel COL5A1 N-propeptide mutation, resulting in an unusual but severe classic EDS phenotype and a remarkable splicing outcome. METHODOLOGY/PRINCIPAL FINDINGS: We identified a novel COL5A1 N-propeptide acceptor-splice site mutation (IVS6-2A>G, NM_000093.3_c.925-2A>G in a patient with cutaneous features of EDS, severe progressive scoliosis and eye involvement. Two mutant transcripts were identified, one with an exon 7 skip and one in which exon 7 and the upstream exon 6 are deleted. Both transcripts are expressed and secreted into the extracellular matrix, where they can participate in and perturb collagen fibrillogenesis, as illustrated by the presence of dermal collagen cauliflowers. Determination of the order of intron removal and computational analysis showed that simultaneous skipping of exons 6 and 7 is due to the combined effect of delayed splicing of intron 7, altered pre-mRNA secondary structure, low splice site strength and possibly disturbed binding of splicing factors. CONCLUSIONS/SIGNIFICANCE: We report a novel COL5A1 N-propeptide acceptor-splice site mutation in intron 6, which not only affects splicing of the adjacent exon 7, but also causes a splicing error of the upstream exon 6. Our findings add further insights into the COL5A1 splicing order and show for the first time that a single COL5A1 acceptor-splice site

Genetic screening of a pedigree with osteogenesis imperfecta type Ⅰand identification of a novel mutation in COL1A2 pathogenic gene%成骨不全Ⅰ型家系的基因检测和COL1A2基因新突变的致病性鉴定

Institute of Scientific and Technical Information of China (English)

李荣; 郭源平; 潘敬新; 郭奕斌

2015-01-01

To uncover the molecular pathogenic mechanism of congenital osteogenesis imperfecta (OI) type I, all the 103 exons of the COL1A1 (Collagen, type Ⅰ, alpha 1) and COL1A2 (Collagen, type Ⅰ, alpha 2) genes in a child with OI type Ⅰ were screened using PCR-DNA direct sequencing. The results showed no pathological mutation in COL1A1 gene, but a novel mutation c.946G>T/p.G316C in the exon 19 of COL1A2 gene, which was inherited from her father. This mutation was not found in her mother and other six phenotypically normal relatives. By denaturing high perfor-mance liquid chromatography (DHPLC) screening, the abnormal double-peak was visualized in PCR products of exon 19 of COL1A2 gene in the proband and her father, while the normal single-peak was shown in those of her mother and all the healthy controls. Using allele specific amplification (ASA) screening, a specific band of 391 bp in COL1A2 exon 19 was amplified only in the proband and her father, but not in other samples. The amino acid encoded by the mutation site is evolutionarily highly conserved, and this mutation was a“damaging”or“probably damaging”factor to OI type Ⅰ, based on the predicting results using SIFT and Polyphen-2 softwares. In conclusion, the novel c.946G>T/p.G316C mutation in COL1A2 gene is a pathogenic mutation that could result in OI type Ⅰ. If the couple wants to get pregnant again, it is necessary to screen the mutation site in COL1A2 gene through the prenatal genetic diagnosis in the first trimester or through preimplantation genetic diagnosis (PGD) in the progestation.%为了揭示成骨不全(Osteogenesis imperfecta, OI)Ⅰ型家系的分子遗传学发生机制，文章采用PCR-DNA直接测序法，对患儿 COL1A1和 COL1A2基因共103个外显子(E)进行突变检测。结果显示：患儿 COL1A1基因未发现任何病理性突变，而在 COL1A2基因 E19内发现一新的杂合错义突变(p.G316C)，该突变来自其父，而其母正常，其他表型正常的6�

Two-step method for curing Escherichia coli of ColE1-derived plasmids

DEFF Research Database (Denmark)

Hove-Jensen, Bjarne

2008-01-01

To cure Escherichia coli for plasmids derived from the ColE1 replicon advantage is taken of the fact that maintenance of this replicon requires a wild-type allele of polA, encoding DNA polymerase I. Curing is achieved by cotransduction of a mutant polA allele with metE::Tn10, fadAB::Tn10 or other...

Investigando a construção de sentidos sobre o ambiente em visitas de crianças a um colégio agrícola

OpenAIRE

Arante, Juliana de Souza Neves

2009-01-01

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Educação Científica e Tecnológica, Florianópolis, 2009 É prática comum, no Colégio Agrícola Senador Carlos Gomes de Oliveira (CASCGO), localizado no município de Araquari - SC, as visitas programadas de turmas de outros colégios para que conheçam como funciona uma escola-fazenda. Para que esta atividade ocorra, o colégio conta com seus próprios alunos como guias dessas visitas. O objetivo principa...

Análise da expressão do colágeno VI na distrofia muscular congênita Analysis of the expression of collagen VI in congenital muscular dystrophy

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Regina Toni Loureiro de Freitas

2005-06-01

Full Text Available A distrofia muscular congênita (DMC compõe um grupo de miopatias caracterizadas por hipotonia e fraqueza muscular notadas já no primeiro ano de vida. A forma de Ullrich é caracterizada por retrações musculares proximais e hiperextensibilidade distal. Cerca de 40% destes pacientes apresentam mutações em um dos genes que codificam as três sub-unidades do colágeno VI (COL6, acarretando deficiência total ou parcial na marcação da proteína. Analisamos, através de imunofluorescência, a marcação do COL6 em fragmentos musculares de 50 pacientes com DMC, 20 deles com ausência da marcação para merosina. Identificamos 4 casos com deficiência total da marcação do COL6 (8% do total, representando 13% dos casos com marcação normal para merosina. As alterações histológicas musculares dos pacientes com COL6 deficiente eram indistinguíveis das outras formas de DMC, porém mais brandas que as observadas na DMC com deficiência de merosina. Em três dos pacientes com COL6 deficiente observou-se hipotonia e fraqueza muscular, notadas já no período neonatal, atraso do desenvolvimento motor, retrações musculares em joelhos e cotovelos, hiperextensibilidade distal e luxação congênita do quadril (dois pacientes. Um paciente perdeu a capacidade para a marcha, e outro faleceu por problemas respiratórios. A análise da marcação do COL6, assim como da merosina, no tecido muscular de pacientes com DMC pode auxiliar na identificação e caracterização fenotípica dos diversos subtipos de DMC.Congenital muscular dystrophy (CMD composes a group of disorders characterized by hypotonia and muscular weakness noticed in the first year of life. The Ullrich's form is characterized by proximal joint contractures and distal hiperextensibility. About 40% of these patients present mutations in one of the genes that codify the sub-units of the collagen VI protein (COL6, producing total or partial deficiency of the protein expression. We analyzed

Carcinome à cellule vitreuse du col de l'utérus: à propos d'un cas et revue de littérature

Science.gov (United States)

Hakimi, Ihssane; Zazi, Abdelghani; Chahdi, Hafsa; Guelzim, Khalid; Kouach, Jaouad; Babahabib, Myabdellah; Elhassani, Myehdi; Rahali, Driss Moussaoui; Dehayni, Mohammed

2015-01-01

Le carcinome à cellule vitreuse du col de l'utérus est un type de histologique rare de cancer du col de l'utérus qui survient à un âge plus jeune, et s'associe au risque élevé d’échec thérapeutique et le pronostic est plus mauvais en comparaison au type cellulaire squameux. La radiothérapie est associée au risque diminué de récidive. Le but de cette étude est de récapituler à travers d'une observation et une revue de littérature les données sur l'incidence, le comportement clinique et la survie globale de patients avec le carcinome à cellule vitreuse du col de l'utérus. PMID:26664556

Views of Astronaut (Col.) Joe Engle and son Jon with L-5 Piper Cub

Science.gov (United States)

1981-01-01

Views of Astronaut (Col.) Joe Engle and son Jon with L-5 Piper Cub at Clover Airport. Photos include Engle turning propeller while his son sits in the cockpit (34323); both Engle and son examine propeller (34324); Engle works on engine while his son sits in cockpit (34325).

Inter-Accions. Una experiència transdisciplinària en un context pedagògic col·lectiu

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Marta Carrasco-Bonet

2015-07-01

Full Text Available Inter-Accions és un projecte pedagògic col·lectiu interuniversitari que treballa des de l'autogestió i la transdisciplinarietat art-arquitectura. És un projecte d’estudiants i per als estudiants d’aquests dos camps que es proposa davant la necessitat d’obrir espais autònoms d’aprenentatge dins la mateixa institució. L’objectiu principal de la proposta és construir un espai d’experimentació on sigui possible treballar sobre la gestió col·lectiva de l’aprenentatge més enllà dels continguts i els resultats. Des d’una perspectiva crítica i en un context acadèmic es proposa aquesta experiència per tal que arquitectes i artistes comparteixin rols i que, a partir del treball col·lectiu i col·laboratiu basat en la idea de codi obert, arribin a establir noves metodologies útils, pràctiques i crítiques aplicables al desenvolupament del seu treball sobre la ciutat. Els projectes que s’han desenvolupat en el marc dels diferents tallers prenen la ciutat com a espai de reflexió i d’intervenció, i permeten arribar a la conclusió que ha estat més determinant l’interès en els mètodes i processos d’aproximació i l’interès en els processos de creació i d’aprenentatge que la voluntat d’aconseguir resultats finalistes. Aquest projecte s’està desenvolupant avui en el marc d’una Assignatura d’Extensió Universitària anomenada “Cartografia Artística” a la Universitat de Barcelona.

Auto-imunidade e colágeno V Autoimmunity and collagen V

Directory of Open Access Journals (Sweden)

Cristiane Carla de Oliveira

2006-06-01

Full Text Available As proteínas da matriz extracelular (MEC e seus componentes estão sendo amplamente estudados na literatura médica, assim como sua relação com o remodelamento tecidual presente nas doenças reumáticas. Neste artigo, mostramos a importância do estudo do colágeno do tipo V no entendimento da etiologia da esclerodermia, no que se refere ao desencadeamento da auto-imunidade nesta enfermidade. Estudos em nosso laboratório demonstram que a sensibilização com colágeno do tipo V em coelhos pode resultar em um modelo animal de esclerodermia. Diante destes fatos, sugerimos que pesquisas neste campo podem ser de grande valia no desenvolvimento de novas condutas terapêuticas.The extracellular matrix (ECM proteins and their components have been widely studied in medical literature, as well its relationship with the tecidual remodeling present in the rheumatic disease. In this paper we show the importance of understanding the role of type V collagen as an important trigger of rheumatic autoimmune diseases. Studies in our laboratory demonstrate that type V collagen sensibilization in rabbits, could result in an animal scleroderma model. In this way we suggested that researches in this field can be worthy in development of new therapeutic procedures.

Washington State Biofuels Industry Development

Energy Technology Data Exchange (ETDEWEB)

Gustafson, Richard [Univ. of Washington, Seattle, WA (United States)

2017-04-09

The funding from this research grant enabled us to design, renovate, and equip laboratories to support University of Washington biofuels research program. The research that is being done with the equipment from this grant will facilitate the establishment of a biofuels industry in the Pacific Northwest and enable the University of Washington to launch a substantial biofuels and bio-based product research program.

Demarcação e Divulgação dos Limites entre o Brasil e a Colômbia

OpenAIRE

Novaes, André Reyes; Palacios, David Ramírez

2015-01-01

Os documentos aqui reproduzidos expressam um momento importante no processo de demarcação dos limites entre o Brasil e a Colômbia. O primeiro é a “Acta da vigésima primeira e última conferência da Comissão Mixta Brasileira Colombiana Demarcadora de Limites”, realizada em 5 de janeiro de 1937 na cidade de Manaus. O segundo é a “Carta geográfica da fronteira do Alto Solimões entre Brasil, Perú e Colômbia”, publicada em 1940 com base nos trabalhos da citada comissão, e disponível na Biblioteca D...

The generation and characterization of novel Col1a1FRT-Cre-ER-T2-FRT and Col1a1FRT-STOP-FRT-Cre-ER-T2 mice for sequential mutagenesis

Directory of Open Access Journals (Sweden)

Minsi Zhang

2015-09-01

Full Text Available Novel genetically engineered mouse models using the Cre-loxP or the Flp-FRT systems have generated useful reagents to manipulate the mouse genome in a temporally-regulated and tissue-specific manner. By incorporating a constitutive Cre driver line into a mouse model in which FRT-regulated genes in other cell types are regulated by Flp-FRT recombinase, gene expression can be manipulated simultaneously in separate tissue compartments. This application of dual recombinase technology can be used to dissect the role of stromal cells in tumor development and cancer therapy. Generating mice in which Cre-ERT2 is expressed under Flp-FRT-mediated regulation would enable step-wise manipulation of the mouse genome using dual recombinase technology. Such next-generation mouse models would enable sequential mutagenesis to better model cancer and define genes required for tumor maintenance. Here, we generated novel genetically engineered mice that activate or delete Cre-ERT2 in response to Flp recombinase. To potentially utilize the large number of Cre-loxP-regulated transgenic alleles that have already been targeted into the Rosa26 locus, such as different reporters and mutant genes, we targeted the two novel Cre-ERT2 alleles into the endogenous Col1a1 locus for ubiquitous expression. In the Col1a1FRT-Cre-ER-T2-FRT mice, Flp deletes Cre-ERT2, so that Cre-ERT2 is only expressed in cells that have never expressed Flp. In contrast, in the Col1a1FRT-STOP-FRT-Cre-ER-T2 mice, Flp removes the STOP cassette to allow Cre-ERT2 expression so that Cre-ERT2 is only expressed in cells that previously expressed Flp. These two new novel mouse strains will be complementary to each other and will enable the exploration of complex biological questions in development, normal tissue homeostasis and cancer.

The generation and characterization of novel Col1a1FRT-Cre-ER-T2-FRT and Col1a1FRT-STOP-FRT-Cre-ER-T2 mice for sequential mutagenesis

Science.gov (United States)

Zhang, Minsi; Kirsch, David G.

2015-01-01

ABSTRACT Novel genetically engineered mouse models using the Cre-loxP or the Flp-FRT systems have generated useful reagents to manipulate the mouse genome in a temporally-regulated and tissue-specific manner. By incorporating a constitutive Cre driver line into a mouse model in which FRT-regulated genes in other cell types are regulated by Flp-FRT recombinase, gene expression can be manipulated simultaneously in separate tissue compartments. This application of dual recombinase technology can be used to dissect the role of stromal cells in tumor development and cancer therapy. Generating mice in which Cre-ERT2 is expressed under Flp-FRT-mediated regulation would enable step-wise manipulation of the mouse genome using dual recombinase technology. Such next-generation mouse models would enable sequential mutagenesis to better model cancer and define genes required for tumor maintenance. Here, we generated novel genetically engineered mice that activate or delete Cre-ERT2 in response to Flp recombinase. To potentially utilize the large number of Cre-loxP-regulated transgenic alleles that have already been targeted into the Rosa26 locus, such as different reporters and mutant genes, we targeted the two novel Cre-ERT2 alleles into the endogenous Col1a1 locus for ubiquitous expression. In the Col1a1FRT-Cre-ER-T2-FRT mice, Flp deletes Cre-ERT2, so that Cre-ERT2 is only expressed in cells that have never expressed Flp. In contrast, in the Col1a1FRT-STOP-FRT-Cre-ER-T2 mice, Flp removes the STOP cassette to allow Cre-ERT2 expression so that Cre-ERT2 is only expressed in cells that previously expressed Flp. These two new novel mouse strains will be complementary to each other and will enable the exploration of complex biological questions in development, normal tissue homeostasis and cancer. PMID:26183214

University of Washington

Data.gov (United States)

Federal Laboratory Consortium — The theme of the University of Washington based Center for Child Environmental Health Risks Research (CHC) is understanding the biochemical, molecular and exposure...

A New COL3A1 Mutation in Ehlers-Danlos Syndrome Vascular Type With Different Phenotypes in the Same Family.

Science.gov (United States)

Cortini, Francesca; Marinelli, Barbara; Romi, Silvia; Seresini, Agostino; Pesatori, Angela Cecilia; Seia, Manuela; Montano, Nicola; Bassotti, Alessandra

2017-04-01

Vascular Ehlers-Danlos syndrome (vEDS) is a rare and severe connective tissue disorder caused by mutations in the collagen type III alpha I chain ( COL3A1) gene. We describe a pathogenetic heterozygous COL3A1 mutation c.3140 G>A, p. Gly1047Asp, identified using next-generation sequencing, in a 40-year-old Italian female. The genetic test performed on her relatives, which present different clinical phenotypes, confirmed that they carry the same mutation in heterozygous state. This finding confirms that mutations causing vEDS have an incomplete penetrance.

A novel nonsteroidal antifibrotic oligo decoy containing the TGF-beta element found in the COL1A1 gene which regulates murine schistosomiasis liver fibrosis.

Science.gov (United States)

Boros, D L; Singh, K P; Gerard, H C; Hudson, A P; White, S L; Cutroneo, K R

2005-08-01

Schistosomiasis mansoni disseminated worm eggs in mice and humans induce granulomatous inflammations and cumulative fibrosis causing morbidity and possibly mortality. In this study, intrahepatic and I.V. injections of a double-stranded oligodeoxynucleotide decoy containing the TGF-beta regulatory element found in the distal promoter of the COL1A1 gene into worm-infected mice suppressed TGF-beta1, COL1A1, tissue inhibitor of metalloproteinase-1, and decreased COL3A1 mRNAs to a lesser extent. Sequence comparisons within the mouse genome found homologous sequences within the COL3A1, TGF-beta1, and TIMP-1 5' flanking regions. Cold competition gel mobility shift assays using these homologous sequences with 5' and 3' flanking regions found in the natural COL1A1 gene showed competition. Competitive gel mobility assays in a separate experiment showed no competition using a 5-base mutated or scrambled sequence. Explanted liver granulomas from saline-injected mice incorporated 10.45 +/- 1.7% (3)H-proline into newly synthesized collagen, whereas decoy-treated mice showed no collagen synthesis. Compared with the saline control schistosomiasis mice phosphorothioate double-stranded oligodeoxynucleotide treatment decreased total liver collagen content (i.e. hydroxy-4-proline) by 34%. This novel molecular approach has the potential to be employed as a novel antifibrotic treatment modality. (c) 2005 Wiley-Liss, Inc.

Loss of col8a1a Function during Zebrafish Embryogenesis Results in Congenital Vertebral Malformations

Science.gov (United States)

Gray, Ryan S.; Wilm, Thomas; Smith, Jeff; Bagnat, Michel; Dale, Rodney M.; Topczewski, Jacek; Johnson, Stephen L.; Solnica-Krezel, Lilianna

2014-01-01

Congenital vertebral malformations (CVM) occur in 1 in 1,000 live births and in many cases can cause spinal deformities, such as scoliosis, and result in disability and distress of affected individuals. Many severe forms of the disease, such as spondylocostal dystostosis, are recessive monogenic traits affecting somitogenesis, however the etiologies of the majority of CVM cases remain undetermined. Here we demonstrate that morphological defects of the notochord in zebrafish can generate congenital-type spine defects. We characterize three recessive zebrafish leviathan/col8a1a mutant alleles (m531, vu41, vu105) that disrupt collagen type VIII alpha1a (col8a1a), and cause folding of the embryonic notochord and consequently adult vertebral column malformations. Furthermore, we provide evidence that a transient loss of col8a1a function or inhibition of Lysyl oxidases with drugs during embryogenesis was sufficient to generate vertebral fusions and scoliosis in the adult spine. Using periodic imaging of individual zebrafish, we correlate focal notochord defects of the embryo with vertebral malformations (VM) in the adult. Finally, we show that bends and kinks in the notochord can lead to aberrant apposition of osteoblasts normally confined to well-segmented areas of the developing vertebral bodies. Our results afford a novel mechanism for the formation of VM, independent of defects of somitogenesis, resulting from aberrant bone deposition at regions of misshapen notochord tissue. PMID:24333517

Over-expression of DSCAM and COL6A2 cooperatively generates congenital heart defects.

Directory of Open Access Journals (Sweden)

Tamar R Grossman

2011-11-01

Full Text Available A significant current challenge in human genetics is the identification of interacting genetic loci mediating complex polygenic disorders. One of the best characterized polygenic diseases is Down syndrome (DS, which results from an extra copy of part or all of chromosome 21. A short interval near the distal tip of chromosome 21 contributes to congenital heart defects (CHD, and a variety of indirect genetic evidence suggests that multiple candidate genes in this region may contribute to this phenotype. We devised a tiered genetic approach to identify interacting CHD candidate genes. We first used the well vetted Drosophila heart as an assay to identify interacting CHD candidate genes by expressing them alone and in all possible pairwise combinations and testing for effects on rhythmicity or heart failure following stress. This comprehensive analysis identified DSCAM and COL6A2 as the most strongly interacting pair of genes. We then over-expressed these two genes alone or in combination in the mouse heart. While over-expression of either gene alone did not affect viability and had little or no effect on heart physiology or morphology, co-expression of the two genes resulted in ≈50% mortality and severe physiological and morphological defects, including atrial septal defects and cardiac hypertrophy. Cooperative interactions between DSCAM and COL6A2 were also observed in the H9C2 cardiac cell line and transcriptional analysis of this interaction points to genes involved in adhesion and cardiac hypertrophy. Our success in defining a cooperative interaction between DSCAM and COL6A2 suggests that the multi-tiered genetic approach we have taken involving human mapping data, comprehensive combinatorial screening in Drosophila, and validation in vivo in mice and in mammalian cells lines should be applicable to identifying specific loci mediating a broad variety of other polygenic disorders.

Washington Tsunami Hazard Mitigation Program

Science.gov (United States)

Walsh, T. J.; Schelling, J.

2012-12-01

Washington State has participated in the National Tsunami Hazard Mitigation Program (NTHMP) since its inception in 1995. We have participated in the tsunami inundation hazard mapping, evacuation planning, education, and outreach efforts that generally characterize the NTHMP efforts. We have also investigated hazards of significant interest to the Pacific Northwest. The hazard from locally generated earthquakes on the Cascadia subduction zone, which threatens tsunami inundation in less than hour following a magnitude 9 earthquake, creates special problems for low-lying accretionary shoreforms in Washington, such as the spits of Long Beach and Ocean Shores, where high ground is not accessible within the limited time available for evacuation. To ameliorate this problem, we convened a panel of the Applied Technology Council to develop guidelines for construction of facilities for vertical evacuation from tsunamis, published as FEMA 646, now incorporated in the International Building Code as Appendix M. We followed this with a program called Project Safe Haven (http://www.facebook.com/ProjectSafeHaven) to site such facilities along the Washington coast in appropriate locations and appropriate designs to blend with the local communities, as chosen by the citizens. This has now been completed for the entire outer coast of Washington. In conjunction with this effort, we have evaluated the potential for earthquake-induced ground failures in and near tsunami hazard zones to help develop cost estimates for these structures and to establish appropriate tsunami evacuation routes and evacuation assembly areas that are likely to to be available after a major subduction zone earthquake. We intend to continue these geotechnical evaluations for all tsunami hazard zones in Washington.

Osteogenesis imperfecta Type IV: a newly identified variant at position c.560 (G > T; p.Gly187Val) in the COL1A2 gene.

Science.gov (United States)

Usta, Akin; Karademir, Dilay; Sen, Eylem; Yazici, Selcuk; Adali, Ertan; Erdem, Erkan; Karacan, Meric

2017-01-01

Osteogenesis imperfecta is a clinically heterogenous disease caused by defective collagen syntesis associated with a mutation in the COL1A1 or COL1A2 genes. In this report, we present a case of osteogenesis imperfecta (OI) type IV, seen in a female fetus with incurved femurs at 18 weeks of gestation. Molecular analysis of the newborn revealed a novel mutation at position c.560 (c.560 G > T) of the exon 12 in the COL1A2 gene; which lead to the glycine modification with valine (p.Gly187Val) at codon 187. The pregnancy follow-up was uneventful. After delivery, the newborn underwent biphosponat therapy and no fracture was detected until 1 year old.

A Search for Warriors: The Effects of Technology on the Air Force Ethos

Science.gov (United States)

1997-04-01

quoted by Oberstleutnant Peter F. Hauser, and Lt Col John Rawls , C., and Maj. John C. Ornduff, “Lessons from the Kriegsakademie: A Reflection of the...Engagement: A Vision for the 21st Century Air Force. Washington DC: Headquarters USAF, November 1996. Hauser, Oberstleutnant Peter F., and Rawls , Lt Col...Modern Strategy from Machiavelli to the Nuclear Age. Princeton, NJ.: Princeton University Press, 1986. “Recent Space Issues and Development.” Air Force

Horizontal gene transfer of a ColV plasmid has resulted in a dominant avian clonal type of Salmonella enterica serovar Kentucky.

Directory of Open Access Journals (Sweden)

Timothy J Johnson

Full Text Available Salmonella enterica continues to be a significant cause of foodborne gastrointestinal illness in humans. A wide variety of Salmonella serovars have been isolated from production birds and from retail poultry meat. Recently, though, S. enterica subsp. enterica serovar Kentucky has emerged as one of the prominent Salmonella serovars isolated from broiler chickens. Recent work suggests that its emergence apparently coincides with its acquisition of a ColV virulence plasmid. In the present study, we examined 902 Salmonella isolates belonging to 59 different serovars for the presence of this plasmid. Of the serovars examined, the ColV plasmid was found only among isolates belonging to the serovars Kentucky (72.9%, Typhimurium (15.0% and Heidelberg (1.7%. We demonstrated that a single PFGE clonal type of S. Kentucky harbors this plasmid, and acquisition of this plasmid by S. Kentucky significantly increased its ability to colonize the chicken cecum and cause extraintestinal disease. Comparison of the completed sequences of three ColV plasmids from S. Kentucky isolated from different geographical locales, timepoints and sources revealed a nearly identical genetic structure with few single nucleotide changes or insertions/deletions. Overall, it appears that the ColV plasmid was recently acquired by a single clonal type S. Kentucky and confers to its host enhanced colonization and fitness capabilities. Thus, the potential for horizontal gene transfer of virulence and fitness factors to Salmonella from other enteric bacteria exists in poultry, representing a potential human health hazard.

Circular RNA profiling reveals that circCOL3A1-859267 regulate type I collagen expression in photoaged human dermal fibroblasts

International Nuclear Information System (INIS)

Peng, Yating; Song, Xiaojing; Zheng, Yue; Wang, Xinyi; Lai, Wei

2017-01-01

Production of type I collagen declines is a main characteristic during photoaging, but the mechanism is still not fully understood. Circular RNAs (circRNAs) are a class of newly identified non-coding RNAs with regulatory potency by sequestering miRNAs like a sponge. It's more stable than linear RNAs, and would be a useful tool for regulation of gene expression. However, the role of circRNAs in collagen expression during photoaging is still unclear. Here we performed deep sequencing of RNA generated from UVA irradiated and no irradiated human dermal fibroblasts (HDFs) and identified 29 significantly differentially expressed circRNAs (fold change ≥ 1.5, P < 0.05), 12 circRNAs were up-regulated and 17 circRNAs were down-regulated.3 most differentially expressed circRNAs were verified by qRT-PCR and the down-regulated circCOL3A1-859267 exhibited the most significantly altered in photoaged HDFs. Overexpression of circCOL3A1-859267 inhibited UVA-induced decrease of type I collagen expression and silencing of it reduced type I collagen intensity. Via a bioinformatic method, 44 miRNAs were predicted to binding with circCOL3A1-859267, 5 of them have been confirmed or predicted to interact with type I collagen. This study show that circCOL3A1-859267 regulate type I collagen expression in photoaged HDFs, suggesting it may be a novel target for interfering photoaging.

Papel del colágeno VI en la homeostasis del músculo esquelético y tejido adiposo: implicaciones en la patofisiología de las distrofias musculares

OpenAIRE

Rodríguez García, Mª Ángeles

2016-01-01

[spa] El colágeno VI es una proteína de la matriz extracelular cuyos defectos conducen a una variedad de trastornos musculares en humanos caracterizados por una debilidad muscular entre otros múltiples síntomas. Tanto el colágeno VI, como su fragmento soluble carboxilo terminal llamado endotrofina se encuentran altamente expresados en los dos principales tejidos periféricos que regulan la glucemia, el músculo esquelético y tejido adiposo. Los niveles de ARNm de colágeno VI en el tejido adipos...

SIRT1 deacetylates RFX5 and antagonizes repression of collagen type I (COL1A2) transcription in smooth muscle cells

Energy Technology Data Exchange (ETDEWEB)

Xia, Jun [Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University (China); Department of Respiratory Medicine, Jiangsu Provincial Hospital of Chinese Traditional Medicine (China); Wu, Xiaoyan; Yang, Yuyu; Zhao, Yuhao [Atherosclerosis Research Center, Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Pathophysiology, Nanjing Medical University (China); Fang, Mingming [Jiangsu Jiankang Vocational Institute (China); Xie, Weiping, E-mail: wpxienjmu@gmail.com [Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University (China); Wang, Hong, E-mail: hwangnjmu@gmail.com [Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University (China); Xu, Yong [Atherosclerosis Research Center, Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Pathophysiology, Nanjing Medical University (China)

2012-11-16

Highlights: Black-Right-Pointing-Pointer SIRT1 interacts with and deacetylates RFX5. Black-Right-Pointing-Pointer SIRT1 activation attenuates whereas SIRT1 inhibition enhances collagen repression by RFX5 in vascular smooth muscle cells. Black-Right-Pointing-Pointer SIRT1 promotes cytoplasmic localization and proteasomal degradation of RFX5 and cripples promoter recruitment of RFX5. Black-Right-Pointing-Pointer IFN-{gamma} represses SIRT1 expression in vascular smooth muscle cells. Black-Right-Pointing-Pointer SIRT1 agonist alleviates collagen repression by IFN-{gamma} in vascular smooth muscle cells. -- Abstract: Decreased expression of collagen by vascular smooth muscle cells (SMCs) within the atherosclerotic plaque contributes to the thinning of the fibrous cap and poses a great threat to plaque rupture. Elucidation of the mechanism underlying repressed collagen type I (COL1A2) gene would potentially provide novel solutions that can prevent rupture-induced complications. We have previously shown that regulatory factor for X-box (RFX5) binds to the COL1A2 transcription start site and represses its transcription. Here we report that SIRT1, an NAD-dependent, class III deacetylase, forms a complex with RFX5. Over-expression of SIRT1 or NAMPT, which synthesizes NAD+ to activate SIRT1, or treatment with the SIRT1 agonist resveratrol decreases RFX5 acetylation and disrupts repression of the COL1A2 promoter activity by RFX5. On the contrary, knockdown of SIRT1 or treatment with SIRT1 inhibitors induces RFX5 acetylation and enhances the repression of collagen transcription. SIRT1 antagonizes RFX5 activity by promoting its nuclear expulsion and proteasomal degradation hence dampening its binding to the COL1A2 promoter. The pro-inflammatory cytokine IFN-{gamma} represses COL1A2 transcription by down-regulating SIRT1 expression in SMCs. Therefore, our data have identified as novel pathway whereby SIRT1 maintains collagen synthesis in SMCs by modulating RFX5 activity.

CNV analysis in Tourette syndrome implicates large genomic rearrangements in COL8A1 and NRXN1.

Directory of Open Access Journals (Sweden)

Abhishek Nag

Full Text Available Tourette syndrome (TS is a neuropsychiatric disorder with a strong genetic component. However, the genetic architecture of TS remains uncertain. Copy number variation (CNV has been shown to contribute to the genetic make-up of several neurodevelopmental conditions, including schizophrenia and autism. Here we describe CNV calls using SNP chip genotype data from an initial sample of 210 TS cases and 285 controls ascertained in two Latin American populations. After extensive quality control, we found that cases (N = 179 have a significant excess (P = 0.006 of large CNV (>500 kb calls compared to controls (N = 234. Amongst 24 large CNVs seen only in the cases, we observed four duplications of the COL8A1 gene region. We also found two cases with ∼400 kb deletions involving NRXN1, a gene previously implicated in neurodevelopmental disorders, including TS. Follow-up using multiplex ligation-dependent probe amplification (and including 53 more TS cases validated the CNV calls and identified additional patients with rearrangements in COL8A1 and NRXN1, but none in controls. Examination of available parents indicates that two out of three NRXN1 deletions detected in the TS cases are de-novo mutations. Our results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions.

CNV analysis in Tourette syndrome implicates large genomic rearrangements in COL8A1 and NRXN1.

Science.gov (United States)

Nag, Abhishek; Bochukova, Elena G; Kremeyer, Barbara; Campbell, Desmond D; Muller, Heike; Valencia-Duarte, Ana V; Cardona, Julio; Rivas, Isabel C; Mesa, Sandra C; Cuartas, Mauricio; Garcia, Jharley; Bedoya, Gabriel; Cornejo, William; Herrera, Luis D; Romero, Roxana; Fournier, Eduardo; Reus, Victor I; Lowe, Thomas L; Farooqi, I Sadaf; Mathews, Carol A; McGrath, Lauren M; Yu, Dongmei; Cook, Ed; Wang, Kai; Scharf, Jeremiah M; Pauls, David L; Freimer, Nelson B; Plagnol, Vincent; Ruiz-Linares, Andrés

2013-01-01

Tourette syndrome (TS) is a neuropsychiatric disorder with a strong genetic component. However, the genetic architecture of TS remains uncertain. Copy number variation (CNV) has been shown to contribute to the genetic make-up of several neurodevelopmental conditions, including schizophrenia and autism. Here we describe CNV calls using SNP chip genotype data from an initial sample of 210 TS cases and 285 controls ascertained in two Latin American populations. After extensive quality control, we found that cases (N = 179) have a significant excess (P = 0.006) of large CNV (>500 kb) calls compared to controls (N = 234). Amongst 24 large CNVs seen only in the cases, we observed four duplications of the COL8A1 gene region. We also found two cases with ∼400 kb deletions involving NRXN1, a gene previously implicated in neurodevelopmental disorders, including TS. Follow-up using multiplex ligation-dependent probe amplification (and including 53 more TS cases) validated the CNV calls and identified additional patients with rearrangements in COL8A1 and NRXN1, but none in controls. Examination of available parents indicates that two out of three NRXN1 deletions detected in the TS cases are de-novo mutations. Our results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions.

Common variants in the COL4A4 gene confer susceptibility to lattice degeneration of the retina.

Science.gov (United States)

Meguro, Akira; Ideta, Hidenao; Ota, Masao; Ito, Norihiko; Ideta, Ryuichi; Yonemoto, Junichi; Takeuchi, Masaki; Uemoto, Riyo; Nishide, Tadayuki; Iijima, Yasuhito; Kawagoe, Tatsukata; Okada, Eiichi; Shiota, Tomoko; Hagihara, Yuta; Oka, Akira; Inoko, Hidetoshi; Mizuki, Nobuhisa

2012-01-01

Lattice degeneration of the retina is a vitreoretinal disorder characterized by a visible fundus lesion predisposing the patient to retinal tears and detachment. The etiology of this degeneration is still uncertain, but it is likely that both genetic and environmental factors play important roles in its development. To identify genetic susceptibility regions for lattice degeneration of the retina, we performed a genome-wide association study (GWAS) using a dense panel of 23,465 microsatellite markers covering the entire human genome. This GWAS in a Japanese cohort (294 patients with lattice degeneration and 294 controls) led to the identification of one microsatellite locus, D2S0276i, in the collagen type IV alpha 4 (COL4A4) gene on chromosome 2q36.3. To validate the significance of this observation, we evaluated the D2S0276i region in the GWAS cohort and in an independent Japanese cohort (280 patients and 314 controls) using D2S0276i and 47 single nucleotide polymorphisms covering the region. The strong associations were observed in D2S0276i and rs7558081 in the COL4A4 gene (Pc = 5.8 × 10(-6), OR = 0.63 and Pc = 1.0 × 10(-5), OR = 0.69 in a total of 574 patients and 608 controls, respectively). Our findings suggest that variants in the COL4A4 gene may contribute to the development of lattice degeneration of the retina.

Common variants in the COL4A4 gene confer susceptibility to lattice degeneration of the retina.

Directory of Open Access Journals (Sweden)

Akira Meguro

Full Text Available Lattice degeneration of the retina is a vitreoretinal disorder characterized by a visible fundus lesion predisposing the patient to retinal tears and detachment. The etiology of this degeneration is still uncertain, but it is likely that both genetic and environmental factors play important roles in its development. To identify genetic susceptibility regions for lattice degeneration of the retina, we performed a genome-wide association study (GWAS using a dense panel of 23,465 microsatellite markers covering the entire human genome. This GWAS in a Japanese cohort (294 patients with lattice degeneration and 294 controls led to the identification of one microsatellite locus, D2S0276i, in the collagen type IV alpha 4 (COL4A4 gene on chromosome 2q36.3. To validate the significance of this observation, we evaluated the D2S0276i region in the GWAS cohort and in an independent Japanese cohort (280 patients and 314 controls using D2S0276i and 47 single nucleotide polymorphisms covering the region. The strong associations were observed in D2S0276i and rs7558081 in the COL4A4 gene (Pc = 5.8 × 10(-6, OR = 0.63 and Pc = 1.0 × 10(-5, OR = 0.69 in a total of 574 patients and 608 controls, respectively. Our findings suggest that variants in the COL4A4 gene may contribute to the development of lattice degeneration of the retina.

Cisto de colédoco: experiência de cinco anos com o tratamento cirúrgico no hospital municipal JESUS-RJ

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Lisieux Eyer de Jesus

Full Text Available OBJETIVO: Relatar a experiência no diagnóstico e tratamento do cisto de colédoco no Hospital Municipal Jesus em cinco anos (1996-2001 e a experiência brasileira publicada para o tratamento do cisto de colédoco em crianças. MÉTODO: Avaliação prospectiva dos pacientes operados com o diagnóstico de cisto de colédoco em cinco anos no Hospital Municipal Jesus. RESULTADOS: Houve predomínio de casos diagnosticados pela presença de dor abdominal recorrente, icterícia e/ou massa abdominal palpável. Apenas dois pacientes apresentavam a tríade completa. Todos os pacientes foram submetidos à ultra-sonografia e houve dúvida no diagnóstico ultra-sonográfico em apenas dois. Todos eram cistos do tipo 1 de Todani tratados através de ressecção completa e anastomose biliodigestiva em Y Roux. Houve complicações pós-operatórias imediatas em três pacientes: um caso de hemorragia digestiva alta e dois casos de fístula biliar, um deles relacionado a problemas técnicos na anastomose que necessitou de reoperação. O seguimento variou de quatro meses a cinco anos, sem detecção de complicação tardia em nenhum caso. CONCLUSÕES: O tratamento do cisto de colédoco através da ressecção completa na criança é seguro, relacionado a poucas complicações e capaz de resolver por completo a sintomatologia pré-operatória dos pacientes.

Role of CTA1R7K-COL-DD as a novel therapeutic mucosal tolerance-inducing vector for treatment of collagen-induced arthritis.

Science.gov (United States)

Hasselberg, Annemarie; Schön, Karin; Tarkowski, Andrej; Lycke, Nils

2009-06-01

To determine whether a cholera toxin-derived, novel immunomodulating fusion protein, CTA1R7K-COL-DD, carrying the class II major histocompatibility complex H-2q-restricted type II collagen peptide aa 259-274, can induce therapeutic tolerance and prevent collagen-induced arthritis (CIA) when administered intranasally in DBA/1 mice, and to assess whether ADP-ribosylation at the mucosal membranes exerts a regulatory function such that the outcome of tolerance or immune enhancement can be controlled. DBA/1 mice with CIA were treated intranasally with CTA1R7K-COL-DD. The therapeutic effect was monitored for 46 days after the onset of disease. Clinical scoring of disease, histologic examination of inflammation, and bone erosion were assessed, and cytokine levels were determined in the serum or supernatants from splenocytes stimulated with recall antigen. The protective effect of CTA1R7K-COL-DD resulted in roughly 60% of the mice having no clinical signs or histologic evidence of disease after treatment, and those with CIA had significantly milder disease with less bone erosion. The protective status was associated with lower serum titers of IgG1, IgG2a, IgG2b, and IgG3 anticollagen and a substantial decrease in the production of interleukin-6 (IL-6), IL-17, and interferon-gamma, while levels of IL-10 were markedly up-regulated both in the serum and at the T cell level. The enzymatically inactive mutant fusion protein CTA1R7K-COL-DD provided substantial therapeutic protection against CIA following intranasal administration. The mechanism behind the effect appears to be mediated by peptide-specific regulatory T cells induced by mucosal exposure to the peptide containing CTA1R7K-COL-DD vector. In addition, ADP-ribosylation at the mucosal membranes acts as a key regulator controlling mucosal tolerance or immunity.

Genetic and Epigenetic Factors at COL2A1 and ABCA4 Influence Clinical Outcome in Congenital Toxoplasmosis

Science.gov (United States)

Jamieson, Sarra E.; de Roubaix, Lee-Anne; Cortina-Borja, Mario; Tan, Hooi Kuan; Mui, Ernest J.; Cordell, Heather J.; Kirisits, Michael J.; Miller, E. Nancy; Peacock, Christopher S.; Hargrave, Aubrey C.; Coyne, Jessica J.; Boyer, Kenneth; Bessieres, Marie-Hélène; Buffolano, Wilma; Ferret, Nicole; Franck, Jacqueline; Kieffer, François; Meier, Paul; Nowakowska, Dorota E.; Paul, Malgorzata; Peyron, François; Stray-Pedersen, Babill; Prusa, Andrea-Romana; Thulliez, Philippe; Wallon, Martine; Petersen, Eskild; McLeod, Rima; Gilbert, Ruth E.; Blackwell, Jenefer M.

2008-01-01

Background Primary Toxoplasma gondii infection during pregnancy can be transmitted to the fetus. At birth, infected infants may have intracranial calcification, hydrocephalus, and retinochoroiditis, and new ocular lesions can occur at any age after birth. Not all children who acquire infection in utero develop these clinical signs of disease. Whilst severity of disease is influenced by trimester in which infection is acquired by the mother, other factors including genetic predisposition may contribute. Methods and Findings In 457 mother-child pairs from Europe, and 149 child/parent trios from North America, we show that ocular and brain disease in congenital toxoplasmosis associate with polymorphisms in ABCA4 encoding ATP-binding cassette transporter, subfamily A, member 4. Polymorphisms at COL2A1 encoding type II collagen associate only with ocular disease. Both loci showed unusual inheritance patterns for the disease allele when comparing outcomes in heterozygous affected children with outcomes in affected children of heterozygous mothers. Modeling suggested either an effect of mother's genotype, or parent-of-origin effects. Experimental studies showed that both ABCA4 and COL2A1 show isoform-specific epigenetic modifications consistent with imprinting. Conclusions These associations between clinical outcomes of congenital toxoplasmosis and polymorphisms at ABCA4 and COL2A1 provide novel insight into the molecular pathways that can be affected by congenital infection with this parasite. PMID:18523590

Genetic and epigenetic factors at COL2A1 and ABCA4 influence clinical outcome in congenital toxoplasmosis.

Directory of Open Access Journals (Sweden)

Sarra E Jamieson

2008-06-01

Full Text Available Primary Toxoplasma gondii infection during pregnancy can be transmitted to the fetus. At birth, infected infants may have intracranial calcification, hydrocephalus, and retinochoroiditis, and new ocular lesions can occur at any age after birth. Not all children who acquire infection in utero develop these clinical signs of disease. Whilst severity of disease is influenced by trimester in which infection is acquired by the mother, other factors including genetic predisposition may contribute.In 457 mother-child pairs from Europe, and 149 child/parent trios from North America, we show that ocular and brain disease in congenital toxoplasmosis associate with polymorphisms in ABCA4 encoding ATP-binding cassette transporter, subfamily A, member 4. Polymorphisms at COL2A1 encoding type II collagen associate only with ocular disease. Both loci showed unusual inheritance patterns for the disease allele when comparing outcomes in heterozygous affected children with outcomes in affected children of heterozygous mothers. Modeling suggested either an effect of mother's genotype, or parent-of-origin effects. Experimental studies showed that both ABCA4 and COL2A1 show isoform-specific epigenetic modifications consistent with imprinting.These associations between clinical outcomes of congenital toxoplasmosis and polymorphisms at ABCA4 and COL2A1 provide novel insight into the molecular pathways that can be affected by congenital infection with this parasite.

Tríplice fronteira Brasil, Peru e Colômbia e as implicações com o narcotráfico

OpenAIRE

Dias Balieiro, Luiz Felipe de Vasconcelos; Nascimento, Izaura Rodrigues

2015-01-01

Na região do Alto Solimões, extremo oeste do Amazonas, localiza-se a chamada Tríplice Fronteira Amazônica, formada pelas cidades de Tabatinga/Brasil, Santa Rosa/Peru e Letícia/Colômbia. Esta zona fronteiriça é identificada como uma das portas de entrada dos entorpecentes oriundos das zonas produtoras do Peru e Colômbia no território brasileiro. Este artigo objetiva promover a análise das características do narcotráfico na tríplice fronteira amazônica, com uma breve exposição do seu processo d...

Participation des médecins généralistes de la province de Benimellal (Maroc) dans le dépistage du cancer du col

Science.gov (United States)

Nani, Samira; Benallal, Mohamed; Hassoune, Samira; Kissi, Dounia; Maaroufi, Abderrahmane

2013-01-01

Introduction Au Maroc, chaque année il y aurait environ 2000 nouveaux cas de cancer du col et les 2/3 des cas sont pris en charge à un stade très avancé. Nous avons mené une étude transversale, exhaustive incluant les 71 médecins généralistes exerçant dans les établissements de soins de santé de base du secteur public et privé de la province de Benimellal. Le but était d’évaluer leurs connaissances et leur participation au dépistage du cancer du col. Méthodes Nous avons mené une étude transversale, exhaustive incluant les 71 médecins généralistes exerçant dans les établissements de soins de santé de base du secteur public et privé de la province de Benimellal. Le but était d’évaluer leurs connaissances et leur participation au dépistage du cancer du col. Résultats Le niveau de connaissance était relativement modeste, 22 médecins généraliste avaient répondu à la question sur l'incidence du cancer du col au Maroc, Parmi eux (81,8%) avaient donné une réponse incorrecte. L'Herpes Papilloma virus comme facteur de risque du cancer du col a été identifié par seulement 21% des médecins généralistes. La participation au dépistage était également défaillante, 92,8% n'avaient jamais pratiqué le FCV chez leurs patientes à cause principalement du manque de formation (95,5%). Conclusion Les résultats montrent la nécessité d'améliorer les connaissances théoriques et pratique des médecins généralistes concernant le dépistage du cancer du col. PMID:23785557

Tempo de reepitelização corneana com a instilação de colírio contendo hialuronato de sódio e carboximetilcelulose

Directory of Open Access Journals (Sweden)

Luciane Bugman Moreira

2013-10-01

Full Text Available OBJETIVO: Avaliar o tempo de reepitelização corneana pós abrasão usando colírios comercialmente disponíveis, um contendo hialuronato de sódio a 0,4%, outro contendo carboximetilcelulose a 1%, e comparar com a reepitelização sem instilação de colírio. MÉTODOS: Foram utilizados 24 coelhos, nos quais foi feita a abrasão mecânica da córnea nos 8 mm centrais. Esses animais foram divididos em três grupos. O primeiro grupo recebeu um colírio disponível comercialmente contendo hialuronato de sódio 0,4%, o segundo recebeu um colírio contendo carboximetilcelulose 1% e o terceiro não recebeu nenhuma droga. A avaliação foi feita a cada 24 horas por meio da análise de fotografias digitais sob luz azul de cobalto e coramento das córneas com fluoresceína a 2%. O estudo das imagens foi feito pelo sistema de análise de imagens do Autocad 2009®. A análise dos dados foi feita comparando o tempo total de reepitelização da córnea e a cada 24 horas entre os três grupos. RESULTADOS: A velocidade de reepitelização do grupo que usou colírio contendo hialuronato de sódio foi em média 90 horas; o grupo que usou carboximetilcelulose apresentou média de 105 horas; e o grupo que não usou nenhum tipo de lubrificante apresentou média de 108 horas para total reepitelização. Houve uma melhor performance na reepitelização após 96 horas nas córneas dos coelhos que usaram os colírios lubrificantes, sendo essa diferença estatisticamente comprovada. CONCLUSÃO: O colírio contendo hialuronato de sódio 0,4% mostrou índice de eficácia maior que aquele contendo carboximetilcelulose 1%, e este maior eficácia que o controle. Os resultados encontrados neste estudo mostram que o uso de lubrificantes no processo de reepitelização são de extrema valia e devem ser usados de rotina na clínica oftalmológica.

Colágeno asimilable. Fuente de prevención de enfermedades osteoarticuladas

OpenAIRE

Parra Parra, Yolanda; Quesada Martínez, María Inmaculada; Pérez Collado, Elisa María

2015-01-01

Cartel presentado en la Segunda Conferencia Internacional de Comunicación en Salud, celebrada el 23 de octubre de 2015 en la Universidad Carlos III de Madrid Introducción: El colágeno es la proteína más abundante de nuestro cuerpo humano y uno de sus componentes esencial de articulaciones, cartílago, ligamento, tendones, huesos, piel. Su especial estructura lo hacen único, presenta una estructura fibrosa, que aporta gran resistencia y flexibilidad a los tejidos de los que forma parte. Cu...

Distribución espacial de fibroblastos endoneurales en cultivos tridimensionales en geles de colágeno Tipo I

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Leslie Yaneth Leal Mejía

2002-07-01

Full Text Available SaludRESUMENEn la actualidad los cultivos celulares tridimensionales son muy utilizados, ya que imitan lascondiciones in vivode las células. Uno de los cultivos más desarrollados es el de fibroblastosdérmicos dentro de geles de colágeno en forma de lenteja, los cuales se contraen de un 70%a un 80% durante las primeras 24 horas de cultivo. El objetivo inicial de este trabajo fue aislarfibroblastos endoneurales (FE del nervio ciático de ratones ICR, mantenerlos en cultivo enmonocapa y luego en cultivo tridimensional en geles cilíndricos de colágeno tipo I de 1,6 mmde diámetro y 3 mm de largo, formados en las prótesis de silicona empleadas para regeneraciónde nervios periféricos; imitando las condiciones del nervio in vivopara que pueda ser emplea-dos en regeneración. Inicialmente se retira epineuro y perineuro del nervio, dejando sólo elendoneuro que se disocia con colagenasa (500U/ml y luego se le hace una disociación me-cánica. Las células libres, se cultivan en monocapa con DMEM suplementado con 20% suerobovino fetal, logrando un 95% de pureza en cultivo primario; en los subcultivos se purifican yamplifican los FE. Estos se aislan y se mezclan con una solución de colágeno tipo I a 0,5mg/mlpara la formación de los geles, teniendo en cuenta que cada gel contenga 50.000 fibroblastos,se introduce la mezcla dentro de las prótesis de silicona y se dejan gelificar, siguiendo el mismoprocedimiento se formaron geles sin células; una vez formados los geles se liberan de las cáma-ras y se cultivan en suspensión 1, 3, 5, 7, 15 y 30 días. Al cumplirse cada uno de los tiemposde cultivo, se analizó la apariencia del colágeno y la distribución de los FE en microscopíaóptica y de fluorescencia. Los cultivos primarios de FE se obtuvieron a los 5 días, lograndoacortar el tiempo de confluencia entre 15 y 20 días con respecto a lo reportado en la literatura;un dato novedoso fue la purificación total de los FE en el primer subcultivo

Association of COL1A1 rs1800012 polymorphism with musculoskeletal degenerative diseases: a meta-analysis.

Science.gov (United States)

Zhong, Binlong; Huang, Donghua; Ma, Kaige; Deng, Xiangyu; Shi, Deyao; Wu, Fashuai; Shao, Zengwu

2017-09-26

It has been reported that the single nucleotide polymorphism (SNP) rs1800012 in COL1A1 gene might be linked to the susceptibility of musculoskeletal degenerative diseases, such as osteoarthritis (OA) and intervertebral disc degeneration (IVDD). However, the data from different studies is contradictory. Here we aimed to comprehensively summarize and clarify the relationship between the SNP and musculoskeletal degenerative diseases. Seven eligible studies including 1339 cases and 5406 controls were screened out from PubMed, Web Of Science and Cochrane library databases. Significant association was identified in sub group analysis of IVDD in homozygote model (GG versus TT: OR = 0.33, 95% CI 0.14-0.78, P = 0.012), heterozygote model (GT versus TT: OR = 0.29, 95% CI 0.11-0.72, P = 0.008) and dominant model (GG/GT versus TT: OR = 0.31, 95% CI 0.13-0.74, P = 0.008). Additionally, significant relationship was also found in sub group analysis of severe degree of IVDD in homozygote model (GG versus TT: OR = 0.37, 95% CI 0.15-0.91, P = 0.031), heterozygote model (GT versus TT: OR = 0.33, 95% CI 0.13-0.87, P = 0.024) and dominant model (GG/GT versus TT: OR = 0.36, 95% CI 0.14-0.88, P = 0.025). Although no significance was observed, there is a trend that the more G allele at COL1A1 rs1800012 site, the less possibility of IVDD and severe IVDD would happen. Our results indicate that COL1A1 rs1800012 polymorphism associates with the susceptibility of IVDD. However, this polymorphism may not be associated with OA risk.

COL1A2 gene analysis in a Czech osteogenesis imperfecta patient: a candidate novel mutation in a patient affected by osteogenesis imperfecta type 3

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Hrušková L

2015-08-01

Full Text Available Lucie Hrušková,1 Ivo Mařík,2,3 Stella Mazurová,1 Pavel Martásek,1 Ivan Mazura1 1Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic; 2Ambulant Centre for Defects of Locomotor Apparatus 1.1.c., Prague, Czech Republic; 3Faculty of Medical Studies, West Bohemia University, Pilsen, Czech RepublicAbstract: Osteogenesis imperfecta is a heritable bone fragility disease with a heterogenic genetic origin. Most cases result from mutations of either the COL1A1 gene or the COL1A2 gene. We identified a novel COL1A2 gene mutation in a Czech patient, born to unaffected parents, who was diagnosed according to clinical and anthropometric findings and radiographic features as having type 3 osteogenesis imperfecta, which is a severe form of this disease. The identified Gly814Trp mutation was predicted by a number of complementary bioinformatic programs to result in functional alteration of the protein. This case report provides both evidence of a novel COL1A2 mutation resulting in type 3 osteogenesis imperfecta and a genotype:phenotype correlation in this affected individual. Keywords: osteogenesis imperfecta type 3, collagen, alpha-2 (I chain, substitution, sequencing 

Whole exome analysis identifies dominant COL4A1 mutations in patients with complex ocular phenotypes involving microphthalmia.

Science.gov (United States)

Deml, B; Reis, L M; Maheshwari, M; Griffis, C; Bick, D; Semina, E V

2014-11-01

Anophthalmia/microphthalmia (A/M) is a developmental ocular malformation defined as complete absence or reduction in size of the eye. A/M is a heterogenous disorder with numerous causative genes identified; however, about half the cases lack a molecular diagnosis. We undertook whole exome sequencing in an A/M family with two affected siblings, two unaffected siblings, and unaffected parents; the ocular phenotype was isolated with only mild developmental delay/learning difficulties reported and a normal brain magnetic resonance imaging (MRI) in the proband at 16 months. No pathogenic mutations were identified in 71 known A/M genes. Further analysis identified a shared heterozygous mutation in COL4A1, c.2317G>A, p.(Gly773Arg) that was not seen in the unaffected parents and siblings. Analysis of 24 unrelated A/M exomes identified a novel c.2122G>A, p.(Gly708Arg) mutation in an additional patient with unilateral microphthalmia, bilateral microcornea and Peters anomaly; the mutation was absent in the unaffected mother and the unaffected father was not available. Mutations in COL4A1 have been linked to a spectrum of human disorders; the most consistent feature is cerebrovascular disease with variable ocular anomalies, kidney and muscle defects. This study expands the spectrum of COL4A1 phenotypes and indicates screening in patients with A/M regardless of MRI findings or presumed inheritance pattern. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Father Secchi Goes to Washington

Science.gov (United States)

McCarthy, M. F.

1994-12-01

In 1848 a small group of Jesuit refugees arrived at Georgetown College near Washington, D.C. Among them was a young priest, Angelo Secchi, who had finished theology studies in Rome, but had not been able to complete his final examinations. This done successfully, Secchi turned to astronomy and the new facilities of the Georgetown College Observatory, directed by its founder, Fr. James Curley. During his two years in Washington, Secchi studied physics, wrote an article on Electrical Rheometry for the Smithsonian Institution, and formed a friendship with Matthew Fontaine Maury of the U.S. Navy, who headed the Chart Service and in 1844 was named superintendent of the National Observatory. This was later named the U.S. Naval Observatory. Secchi's friendships formed during the Washington visit proved most helpful for relations between European astronomers and U.S. colleagues. Secchi, after his return to Rome constructed the Observatory of the Collegio Romano atop the baroque Church of St. Ignatius in Rome and began his work in spectral classification of stars.

Corrections Education. Washington's Community and Technical Colleges

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Washington State Board for Community and Technical Colleges, 2015

2015-01-01

The Washington State Department of Corrections contracts with community colleges to provide basic education and job training at each of the state's 12 adult prisons so upon release, individuals are more likely to get jobs and less likely to return. Washington State community colleges build a bridge for offenders to successfully re-enter…

Aerospace Training. Washington's Community and Technical Colleges

Science.gov (United States)

Washington State Board for Community and Technical Colleges, 2014

2014-01-01

Aerospace is an economic powerhouse that generates jobs and fuels our economy. Washington's community and technical colleges produce the world-class employees needed to keep it that way. With about 1,250 aerospace-related firms employing more than 94,000 workers, Washington has the largest concentration of aerospace expertise in the nation. To…

76 FR 16323 - Irish Potatoes Grown in Washington; Continuance Referendum

Science.gov (United States)

2011-03-23

...; FV11-946-1 CR] Irish Potatoes Grown in Washington; Continuance Referendum AGENCY: Agricultural... conducted among eligible Washington potato growers to determine whether they favor continuance of the marketing order regulating the handling of Irish potatoes grown in Washington. DATES: The referendum will be...

75 FR 14462 - Notice of Inventory Completion: Central Washington University, Department of Anthropology...

Science.gov (United States)

2010-03-25

... Washington University, Department of Anthropology, Ellensburg, WA, and Thomas Burke Memorial Washington State... Washington University, Department of Anthropology, Ellensburg, WA, and the Thomas Burke Memorial Washington... Anthropology, Central Washington University, Ellensburg, WA 98926-7544, telephone (509) 963-2671 or Dr. Peter...

Purification of supercoiled DNA of plasmid Col E1 by RPC-5 chromatography

Energy Technology Data Exchange (ETDEWEB)

Best, A.N.; Allison, D.P.; Novelli, G.D.

1981-07-01

Col E1 DNA can be purified to a high degree by RPC-5 chromatography of a partially purified cell lysate with a very shallow linear NaC1 gradient at pH 7.8. Electron micrographs demonstrated that the purest fractions were composed of 93% supercoiled (form I) DNA and 7% open circular (form II) DNA. The actual chromatography can be accomplished in 13 to 14 h and is designed for the production of several milligrams of plasmid DNA.

76 FR 377 - Land Acquisitions; Cowlitz Indian Tribe of Washington

Science.gov (United States)

2011-01-04

... State of Washington by Auditor's File Nos. G 450664 and G 147358. Parcel II That portion of the... thereof acquired by the State of Washington by deed recorded under Auditor's File Nos. G 140380 and D... recorded under Auditor's File No. F 38759, records of Clark County, Washington, described as follows...

Asymptomatic parental mosaicism for osteogenesis imperfect associated with a new splice site mutation in COL1A2

DEFF Research Database (Denmark)

Frederiksen, Anja Lisbeth; Dunø, Morten; Johnsen, Iben Birgit Gade

2016-01-01

Recurrent lethal perinatal osteogenesis imperfecta may result from asymptomatic parental mosaicism. A previously unreported mutation in COL1A2 leads to recurrent cases of fetal osteogenesis imperfecta Sillence type IIA, which emphasizes the importance of clinical and genetic evaluation of mosaicism...

Recent developments: Washington focus

International Nuclear Information System (INIS)

Anon.

1990-01-01

November was a quiet month in Washington. Although Congress has recessed until 1991, the Senate filled vacancies in party leadership positions created by November's elections. The House is expected to proceed with its changes in early December. The Nuclear Energy Forum was held in Washington, DC on November 11-14 to discuss the status of the nuclear industry in the USA. The Forum, held in conjunction with the American Nuclear Society's annual meeting, assembled a large number of CEO's from US, European, and Far Eastern utilities and vendors. The meeting concluded with an announcement by Philip Bayne, President of NYPA and chairman of the Nuclear Power Oversight Committee (NPOC), of the results of a year-long NPOC study entitled a open-quotes Strategic Plan for Building New Nuclear Power Plants.close quotes

Genetic associations of body composition, flexibility and injury risk with ACE, ACTN3 and COL5A1 polymorphisms in Korean ballerinas

Science.gov (United States)

Kim, Jun Ho; Jung, Eun Sun; Kim, Chul-Hyun; Youn, Hyeon; Kim, Hwa Rye

2014-01-01

[Purpose] The purpose of this study was to exam the association of body composition, flexibility, and injury risk to genetic polymorphisms including ACE ID, ACTN3 RX, and COL5A1 polymorphisms in ballet dancers in Korea. [Methods] For the purpose of this study, elite ballerinas (n = 97) and normal female adults (n = 203) aged 18 to 39 were recruited and these participants were tested for body weight, height, body fat, fat free mass, flexibility, injury risks on the joints and gene polymorphisms (ACE, ACTN3, COL5A1 polymorphism). [Results] As results, the ACE DD genotype in ballerinas was associated with higher body fat and percentage of body fat than the ACE II and ID genotypes (p sports injuries, the ankle injury of the XX-genotyped ballerinas was in significantly more prevalence than the RR and XX-genotyped ballerinas (p < 0.05). According to the odd ratio analysis, XX-genotyped ballerinas have the injury risk on the ankle about 4.7 (95% CI: 1.6~13.4, p < 0.05) times more than the RR and RX-genotyped ballerinas. Meanwhile, the COL5A1 polymorphism in ballerinas has no association with any factors including flexibility and injury risks. [Conclusion] In conclusion, ACE polymorphism and ACTN3 polymorphism were associated with ballerinas' performance capacity; COL5A1 was not associated with any factors of performance of Ballerinas. The results suggested that the ACE DD genotype is associated with high body fat, the ACTN3 XX genotype is associated with low fat-free mass, low flexibility, and higher risk of ankle-joint injury. PMID:25566457

Opportunities for addressing laminated root rot caused by Phellinus sulphuracens in Washington's forests: A Report from the Washington State Academy of Sciences in cooperation with the Washington State Department of Natural Resources

Science.gov (United States)

R. James Cook; Robert L. Edmonds; Ned B. Klopfenstein; Willis Littke; Geral McDonald; Daniel Omdahl; Karen Ripley; Charles G. Shaw; Rona Sturrock; Paul Zambino

2013-01-01

This report from the Washington State Academy of Sciences (WSAS) is in response to a request from the Washington State Department of Natural Resources (DNR) to "identify approaches and opportunities ripe for research on understanding and managing root diseases of Douglas-fir." Similar to the process used by the National Research Council, the WSAS upon...

Desenvolvimento de colônias de abelhas com diferentes alimentos protéicos Development of honeybee colonies under protein diets

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Fábia de Mello Pereira

2006-01-01

Full Text Available O objetivo deste trabalho foi avaliar o desempenho de produtos regionais do Nordeste na alimentação de colônias de abelhas (Apis mellifera, em um período de escassez de floradas. Foram fornecidas dietas às abelhas, contendo 20% de proteína bruta, à base de feno de mandioca (Manihot esculenta e farinha de vagem de algaroba (Prosopis juliflora, feno de mandioca e farelo de babaçu (Orbygnia martiana, farelo de babaçu e Purilac (sucedâneo para bezerros da marca Purina e pólen apícola de Palmae. As colônias foram analisadas quanto ao peso e às áreas de alimento e cria. Não foi observada diferença significativa entre os tratamentos em relação às áreas de cria. Apesar de a pasta com pólen ser a mais consumida, este alimento mostrou conversão alimentar menor do que as demais dietas fornecidas. As colônias que receberam pasta de feno de mandioca com farelo de babaçu tiveram maior peso final. Todos os alimentos mostraram-se eficientes na manutenção das colônias.The objective of this work was to evaluate the efficiency of some regional products of Brazil Northeast to feed Apis mellifera colonies. Diets with 20% of crude protein made of cassava hay (Manihot esculenta and mesquite pod meal (Prosopis juliflora, cassava hay and babassu bran (Orbygnia martiana, babassu bran and Purilac (succedaneous for calfskin from Purina and Palmae pollen were offered to the honeybees. Colonies were evaluated for weight gain, store area and brood area. There was no significant difference among the treatments in relation to the brood areas. Pollen treatment showed the highest intake but also showed the lowest food conversion. Beehives that received diet with cassava hay and babassu flour showed greater final weight gain. All diets were efficient in the maintenance of the colonies.

Perdas de colônias de Apis mellifera L. no inverno suplementadas com alimentação artificial com pólen e favos de mel

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Adhemar Pegoraro

2012-11-01

Full Text Available O objetivo deste trabalho foi avaliar o efeito do tipo de suplementação, nas áreas de cria, alimento e infestação por Varroa destructor nas colônias perdidas. Foram analisadas18 colônias de Apis mellifera L. alojadas em colméias modelo Langstroth e submetidas a dois tipos de suplementação alimentar: 1- cremosa (açúcar cristal moído, mel e pólen; 2- favos de mel. As áreas de ovo-larva e pupa, néctar, mel e pólen das 18 colônias foram fotografadas e analisadas com o auxílio do software AutoCAD. As colônias perdidas suplementadas com alimento artificial e pólen possuíam áreas de: ovo-larva (87,79 ± 70,38cm2, pupa (228,46 ± 192,94cm2, néctar (1.219 ± 149,58cm2, mel (563,47 ± 807,93cm2, pólen (139,09 ± 80,30cm2 e percentagem de infestação por V. destructor (10,50 ± 4,30%. As colônias perdidas suplementadas com favos de mel possuíam áreas de ovo-larva (7,92 ± 9,35cm2, pupas (60,71 ± 63,94 cm2, néctar (658,22 ± 1.040,94cm2, mel (122,62 ± 49,45cm2, pólen (53,58 ± 72,39cm2 e percentagem de infestação por V. destructor (14,40 ± 1,56%. As causas das perdas em parte podem ser atribuídas à infestação por V. destructor.

Avaliação e monitoramento de implantes ósseos de arcabouços de PLDLA imobilizados com colágeno, préviamente cultivados com osteoblastos

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Paula Galhano Filler

2014-10-01

Full Text Available Introdução: Polímeros biorreabsorvíveis, como PLDLA, são amplamente empregados como arcabouços na engenharia tecidual óssea. Incentiva-se sua interação com macromoléculas naturais da matriz extracelular, como o colágeno, para melhor adesão e reconhecimento do material implantado, e maior neoformação óssea. Objetivo: Analisar e comparar a regeneração óssea através de implantes realizados na calota craniana de ratos. Metodologia: Utilizou-se ratos Wistar de aproximadamente 2 meses, e tempos de sacrifício de 8 e 12 semanas, divididos nos grupos: defeito vazio; PLDLA-Col sem osteoblastos; e PLDLA-Col com osteoblastos. Paralelamente, estudou-se a fluorescência PKH26 em animais de 4 semanas, para avaliar a capacidade dos arcabouços de suportar o crescimento celular in vitro, Resultados: A analise histológica dos tempos de 8 e 12 semanas demonstrou neoformação óssea acentuada e irregular nos implantes de PLDLA-Col com ou sem célula, e fechamento parcial da lesão na maioria dos casos. Além disso, observou-se crescimento ósseo sem relação com as bordas do defeito. A histomorfometria de ambos os tempos apresentou o seguinte resultado decrescente em relação a área óssea neoformada: tratamento > controle positivo > controle negativo. Conclusão: Arcabouços de PLDLA-Col, previamente ou não cultivados com osteoblastos, obtiveram resultados promissores quando comparados ao defeito vazio, confirmando sua importante utilidade na engenharia tecidual.

Synthesis and Characterization of Hydroxyapatite-Collagen-Chitosan (HA/Col/Chi) Composite Coated on Ti6Al4V

Science.gov (United States)

Charlena; Bikharudin, Ahmad; Wahyudi, Setyanto Tri

2018-01-01

HA-collagen-chitosan (HA/col/chi) composite is developed to increase bioactivity adhesiveness between the metal and the material composite and to improve corrosion resistance. The Ti6Al4V alloy was coated by soaking in HA/col/chi composite at room temperature and then allowed to stand for 5, 6, and 7 days. Diffraction pattern analysis of the coated Ti6Al4V alloy showed that the dominant phase were HA and Ti6Al4V alloy. Corrosion resistance test in media by using 0.9% NaCl showed the corrosion rate at the level of 0.3567 mpy, which was better than that of the uncoated Ti6Al4V alloy (0.4152 mpy). In vitro cytocompatibility assay on endothelial cell of calf pulmonary artery endothelium (CPAE) (ATCC-CCL 209) showed there was no toxicity in the cell culture with the percent inhibition of 33.33% after 72 hours of incubation.

Biblioteca do Colégio Estadual Manoel Vilaverde: um espaço além das quatro paredes

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Maria Aparecida Rodrigues de Souza

2005-07-01

Full Text Available Relato de experiência da prestação de serviços e atividades de leitura desenvolvidas na Biblioteca Domingos Garcia Filho do Colégio Estadual Manoel Vilaverde, em Inhumas – Goiás. Este relato faz um apanhado das estratégias para estímulo à leitura praticadas pela BDGF desde a sua reativação em 2001; ano em que o colégio foi contemplado com mais de 1300 livros pelo Programa de Bibliotecas das Escolas Estaduais do Estado de Goiás. Ilustramos através de fotos a participação efetiva dos alunos nos projetos desenvolvidos pela biblioteca. Durante o período de 2001 a 2004 foram desenvolvidos dois grandes projetos: Minutos de Leitura e o Projeto Pró-melhoria dos recursos pedagógicos do CEM. Projetos estes que envolvem diretamente a biblioteca, responsável pela mediação do usuário com a leitura e a pesquisa.

Drivers' use of marijuana in Washington state : traffic tech.

Science.gov (United States)

2016-08-01

In July 2014, Washington State allowed legal sales of : recreational marijuana. Working with the Washington : Traffic Safety Commission, NHTSA assisted the State in : conducting a roadside study to examine the prevalence : of marijuana use before and...

Genetic Variations of the COL4A1 Gene and Intracerebral Hemorrhage Risk: A Case-Control Study in a Chinese Han Population.

Science.gov (United States)

Lin, Sen; Xia, Chao; He, Sha; Yang, Jie; Li, Hao; Zheng, Jun; Liu, Ming; You, Chao

2018-04-01

To investigate the association between single nucleotide polymorphisms or haplotypes of the COL4A1 gene and the risk of intracerebral hemorrhage (ICH). We conducted a case-control study that included 181 patients from the Chinese Han population with hypertensive ICH and 197 hypertension patients without ICH. Genomic DNA was extracted by DNA extraction kit, and the 6 single nucleotide polymorphism genotypes of the COL4A1 gene were detected with a MassARRAY Analyzer. Unphased 3.1.4 and SPSS 19.0 were used to analyze the association between alleles, genotypes, and haplotypes of the COL4A1 gene and the risk of ICH. Compared with the control group, patients in the ICH group were significantly younger. There were no differences in gender, diabetes, hyperlipidemia, current smoking, and alcohol consumption between the 2 groups. Our association analysis showed that the rs3742207 A, rs11069830 A, and rs679505 A alleles were association factors of the risks of ICH; rs11069830 AA, rs544012 AC, and rs679505 AA genotypes were association factors of the risk of ICH; AA haplotype (rs3742207-rs11069830) was an association factor of the risk of ICH. After adjusting age and gender by multivariate logistic regression, the rs544012 AC and rs679505 AA genotypes were independently associated with the risk of ICH. Our study showed that the rs544012 AC and rs679505 AA genotypes were independently associated with the risk of ICH in the Chinese Han population and that the AA haplotype (rs3742207-rs11069830) in the COL4A1 gene may be related to the risk of ICH in the Chinese Han population; these conclusions need further confirmation in future studies with larger samples. Copyright © 2018. Published by Elsevier Inc.

A familial case of achondrogenesis type II caused by a dominant COL2A1 mutation and "patchy" expression in the mosaic father.

Science.gov (United States)

Forzano, F; Lituania, M; Viassolo, A; Superti-Furga, V; Wildhardt, G; Zabel, B; Faravelli, F

2007-12-01

Achondrogenesis type II (ACG2) is the most severe disorder that can be produced by dominant mutations in COL2A1. We report on four pregnancies of an apparently healthy, nonconsanguineous young couple. The father had scoliosis as a child, and has slight body disproportion with short trunk. The first child was born at 32 weeks and died neonatally. In the second pregnancy, short limbs and fetal hygroma were noted on ultrasound at 17 weeks' gestation. Similar findings were observed in the third fetus. Clinical, radiological, and histological evaluation of the fetuses after termination of the pregnancies showed findings consistent with ACG2. Molecular analysis of genomic DNA extracted from amniotic cells of the second and third fetuses revealed heterozygosity for a 10370G > T missense mutation (G346V) in the COL2A1 gene. This mutation was also found in the father, as a mosaic. The couple had a fourth pregnancy, and at 11 weeks fetal hydrops with a septated cystic hygroma were obvious. DNA from CVS demonstrated the same COL2A1 mutation. (c) 2007 Wiley-Liss, Inc.

Uso da terra e perda de solo na Bacia Hidrográfica do Rio Colônia, Bahia Land use and soil loss in the Colônia River Watershed, Bahia

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Vinícius de A. Silva

2011-03-01

Full Text Available Mudanças no uso da terra muitas vezes potencializam a erosão hídrica acarretando perda de água, solo, nutriente e matéria orgânica dos sistemas agrícolas, razão por que se estimou a perda de solo na bacia hidrográfica do rio Colônia, na Bahia, nos últimos vinte e sete anos, utilizando-se o software SWAT (Soil and Water Assessment Tool. Para tal, procedeu-se à digitalização de mapas temáticos, interpretação de fotografias aéreas de 1975; classificação supervisionada de imagens de satélites de 2002 e produção de mapas de uso da terra. O SWAT foi utilizado na obtenção de mapas temáticos digitais por sub-bacia hidrográfica do rio Colônia, quantificação das perdas de solo em cada sub-bacia e nas formas de usos obtidos por conceito teórico, simulando as inclusões de áreas de preservação permanente, bem como mata em toda a superfície das sub-bacias. Estima-se que, entre 1975 e 2002, a média de perda de solo na bacia hidrográfica do rio Colônia foi de 47 t ha-1 ano-1 e em 2002 a estimativa de perda de solo foi de 46,64 t ha-1 ano-1. Na simulação de um cenário teórico de área de preservação permanente (APP e mata, ocorreu diminuição da média da perda de solo em toda a bacia hidrográfica do rio Colônia de, respectivamente, 9,09 t ha-1 ano-1 e 20,91 t ha-1 ano-1.Land use changes most of the time increases the hydric erosion leading to loss of water, nutrients and organic matter in agricultural systems. Thus, aiming to estimate the soil loss in the watershed of Colonia River, in Bahia, in the last twenty-seven years, the software SWAT (Soil and Water Assessment Tool was used. For the purpose, a digitalization thematic map (Arc View, interpretation of aerial photographs from 1975, supervised classification of 2002 satellite images and a land use map generation were developed. The SWAT software was used for obtaining a digital thematic map for every sub-basin of Colonia River Watershed, soil loss

Preparación, caracterización y aplicación como plataformas bioactivas de materiales compuestos formados por politiofeno y colágeno

OpenAIRE

Illera Bautista, Cristina

2014-01-01

El objetivo del trabajo es preparar mezclas compuestas por una proteína, en este caso colágeno tipo I, y un polímero conductor, poli[2,2’-(3-metil-acetato)tiofeno] (P3TMA), un derivado soluble de politiofeno. Para ello se ensayan diferentes metodologías para la preparación de las películas, tales como drop-casting y spin-coating. La proporción de proteína en las mezclas de colágeno/P3TMA es baja, variando entre el 5% y el 0,5% dependiendo de la técnica de preparación empleada, debido a la...

A inserção acadêmica e esportiva da primeira turma feminina no Colégio Militar do Rio de Janeiro

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Liliana Adiers Lohmann

2006-09-01

Full Text Available http://dx.doi.org/10.1590/S0104-026X2006000300005 Os objetivos deste trabalho são contextualizar o ambiente em que se deu a inserção da primeira turma feminina no Colégio Militar do Rio de Janeiro, em 1989, bem como identificar e analisar as representações femininas e masculinas sobre a inserção acadêmica e esportiva dessa primeira turma. A metodologia, qualitativa e etnográfica, fundamenta-se na teoria das representações sociais.1 Os dados provêm da observação participante e de seis entrevistas individuais não-diretivas de “elite”2: três ex-alunas e três homens. Analisam-se também reportagens de jornais e revistas, de 1989 a 1996. Constata-se que cuidado e diferença são constantes no discurso dos dois grupos de informantes. Conclui-se que o incentivo ao esporte, proposto pelo comandante do Colégio e liderado pela professora de Educação Física coautora deste trabalho, influenciou e consolidou o espaço para a participação e integração feminina no Colégio e para o seu êxito acadêmico e esportivo.

Collimation and material science studies (ColMat) at GSI.

CERN Document Server

Stadlmann, J; Kollmus, H; Krause, M; Mustafin, E; Petzenhauser, I; Spiller, P; Strasik, I; Tahir, N; Tomut, M; Trautmann, C

2010-01-01

Within the frame of the EuCARD program, the GSI Helmholtzzentrum für Schwerionenforschung in Darmstadt is performing accelerator R&D in workpackage 8: ColMat. The coordinated effort is focussed on materials aspects important for building the FAIR accelerator facility at GSI and the LHC upgrade at CERN. Accelerator components and especially protection devices have to be operated in high dose environments. The radiation hazard occurs either by the primary proton and ion beams or the secondary radiation after initial beam loss. Detailed numerical simulations have been carried out to study the damage caused to solid targets by the full impact of the LHC beam as well as the SPS beam. Tungsten, copper and graphite as possible collimator materials have been studied. Experimental an theoretical studies on radiation damage on materials used for the LHC upgrade and the FAIR accelerators are performed at the present GSI experimental facilities. Technical decisions based on these results will have an impact on the F...

Delineation of Ehlers-Danlos syndrome phenotype due to the c.934C>T, p.(Arg312Cys) mutation in COL1A1: Report on a three-generation family without cardiovascular events, and literature review.

Science.gov (United States)

Colombi, Marina; Dordoni, Chiara; Venturini, Marina; Zanca, Arianna; Calzavara-Pinton, Piergiacomo; Ritelli, Marco

2017-02-01

Classical Ehlers-Danlos syndrome (cEDS) is a rare connective tissue disorder primarily characterized by hyperextensible skin, defective wound healing, abnormal scars, easy bruising, and generalized joint hypermobility; arterial dissections are rarely observed. Mutations in COL5A1 and COL5A2 encoding type V collagen account for more than 90% of the patients so far characterized. In addition, cEDS phenotype was reported in a small number of patients carrying the c.934C>T mutation in COL1A1 that results in an uncommon substitution of a non-glycine residue in one Gly-Xaa-Yaa repeat of the pro-α1(I)-chain p.(Arg312Cys), which leads to disturbed collagen fibrillogenesis due to delayed removal of the type I procollagen N-propeptide. This specific mutation has been associated with propensity to arterial rupture in early adulthood; indeed, in literature the individuals harboring this mutation are also referred to as "(classic) vascular-like" EDS patients. Herein, we describe a three-generation cEDS family with six adults carrying the p.(Arg312Cys) substitution, which show a variable and prevalent cutaneous involvement without any major vascular event. These data, together with those available in literature, suggest that vascular events are not a diagnostic handle to differentiate patients with the p.(Arg312Cys) COL1A1 mutation from those with COL5A1 and COL5A2 defects, and highlight that during the diagnostic process the presence of at least the p.(Arg312Cys) substitution in COL1A1 should be investigated in cEDS patients without type V collagen mutations. Nevertheless, for these patients, as well as for those affected with cEDS, a periodical vascular surveillance should be carried out together with cardiovascular risk factors monitoring. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Expressão citofotométrica da Caspase-3 no carcinoma papilífero da tireóide e no bócio colóide

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Luciana Rodrigues Queiroz de Souza

Full Text Available OBJETIVO: Descrever a expressão citofotométrica quantitativa do marcador caspase-3 no bócio colóide e no carcinoma papilífero da tireóide e comparar a imunoexpessão entre as doenças. MÉTODOS: Realizou-se estudo imunoistoquímico da proteína caspase-3 em 17 blocos de parafina de carcinoma papilífero da tiróide e 20 de bócio colóide, através do sistema SAMBA 4000 - (Sistema de análise microscópica de busca automática, objetivando-se analisar duas variáveis: índice de marcagem e densidade óptica. RESULTADOS: Houve diferença significativa quanto ao índice de marcagem da caspase-3, entre o bócio colóide e o carcinoma papilífero, sendo maior no carcinoma, e não foi encontrada diferença significativa quanto à densidade óptica. Para o bócio colóide, o coeficiente de correlação estimado entre o índice de marcagem e a densidade óptica foi igual a 0,72, indicando assim, a rejeição da hipótese nula (p <0,001, afirmando-se que existe associação positiva e significativa entre o índice de marcagem e a densidade óptica da caspase-3. Para o carcinoma papilífero da tiróide, o coeficiente de correlação estimado entre o índice de marcagem e a densidade óptica 3 foi de 0,34. O resultado do teste estatístico indicou que não se pode afirmar que existe associação entre esses parâmetros. CONCLUSÃO: Para o bócio colóide existe associação positiva e significativa entre as duas variáveis, índice de marcagem e a densidade óptica da caspase-3, enquanto que para o carcinoma papilífero não existe essa associação. O estudo comparativo entre a análise quantitativa da caspase-3, demonstrou que a apoptose é mais evidente no carcinoma papilífero do que no bócio colóide.

Washington biofuel feedstock crop supply under output price and quantity uncertainty

International Nuclear Information System (INIS)

Zheng Qiujie; Shumway, C. Richard

2012-01-01

Subsidized development of an in-state biofuels industry has received some political support in the state of Washington, USA. Utilizing in-state feedstock supplies could be an efficient way to stimulate biofuel industries and the local economy. In this paper we estimate supply under output price and quantity uncertainty for major biofuel feedstock crops in Washington. Farmers are expected to be risk averse and maximize the utility of profit and uncertainty. We estimate very large Washington price elasticities for corn and sugar beets but a small price elasticity for a third potential feedstock, canola. Even with the large price elasticities for two potential feedstocks, their current and historical production levels in the state are so low that unrealistically large incentives would likely be needed to obtain sufficient feedstock supply for a Washington biofuel industry. Based on our examination of state and regional data, we find low likelihood that a Washington biofuels industry will develop in the near future primarily using within-state biofuel feedstock crops. - Highlights: ► Within-state feedstock crop supplies insufficient for Washington biofuel industry. ► Potential Washington corn and sugar beet supplies very responsive to price changes. ► Feedstock supplies more responsive to higher expected profit than lower risk. ► R and D for conversion of waste cellulosic feedstocks is potentially important policy.

An assessment of interstate safety investment properties in Washington state.

Science.gov (United States)

2014-12-01

The Washington State Department of Transportation (WSDOT) commissioned the current study, targeting the entire interstate : mainline network in Washington State, to provide strategic direction to multi-biennial investment interstate locations that of...

Expanding Access and Opportunity: The Washington State Achievers Program

Science.gov (United States)

Ramsey, Jennifer; Gorgol, Laura

2010-01-01

In 2001, the Bill & Melinda Gates Foundation launched a 10-year, multi-million dollar initiative, the Washington State Achievers Program (WSA), to increase opportunities for low-income students to attend postsecondary institutions in Washington State. The Bill & Melinda Gates Foundation granted funds to the College Success Foundation…

Expanding Access and Opportunity: The Washington State Achievers Scholarship

Science.gov (United States)

O'Brien, Colleen

2011-01-01

In 2001, the Bill & Melinda Gates Foundation launched the multi-year, multi-million dollar Washington State Achievers Scholarship program. Concerned about disparities in college participation for low-income students in the state of Washington versus their wealthier peers, the Gates Foundation partnered with the College Success Foundation…

Acesso endoscópico transepto-interforniceal para cistos colóides: relato de caso

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VIALOGO JOSUÉ GUIMARÃES GRANHA

2000-01-01

Full Text Available A abordagem endoscópica de cistos colóides ainda é matéria controversa. As opções de tratamento são variadas: observação com exames seriados, tratamento da hidrocefalia, aspiração por estereotaxia, microcirurgia e endoscopia. O autor relata o caso de um paciente com cisto colóide do terceiro ventrículo, localizado em posição retroforaminal, no teto diencefálico, dissecando os dois fornices projetando-se supero-posteriormente em direção ao assoalho do ventrículo lateral, por dentro do cavum do septo pelúcido. A lesão foi alcançada com neuroendoscópio rígido, através de um orifício de trépano pré-coronal situado a 2,5 cm da linha média (ponto de Kocher. Seguindo por via transventricular, a lâmina direita do septo pelúcido foi aberta, posterior à veia septal. A lesão, muito endurecida, situada entre os dois fórnices, foi parcialmente ressecada através da via endoscópica transventricular transepto-interforniceal. Realizamos também septostomia e terceiro ventriculostomia endoscópicas neste paciente. Os diferentes tipos de procedimentos cirúrgicos das patologias situadas no terceiro ventrículo são discutidos com ênfase no papel da endoscopia.

The Erosion of Public Trust: Normalization of Deviance In The Air Force

Science.gov (United States)

2016-02-13

Institute of Business Ethics, February 2012, 4-36. Eliason Col William T., ed. “Military Professionalism,” Joint Forces Quarterly 62. Washington DC...accountid=4332 Kramer, Ronald C. and Diane Vaughan, “The Normalization of Deviance," In Encyclopedia of Criminological Theory, edited by Francis T. Cullen

Celularidade do leite e Unidades Formadoras de Colônias nas mastites causadas por Staphylococcus coagulase positiva e coagulase negativa

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Helio Langoni

2015-06-01

Full Text Available Resumo: A mastite é a principal afecção do gado destinado à produção leiteira, que impacta significativamente a cadeia produtiva do leite, com reflexos ainda para a saúde pública. Estudou-se aspectos relacionados à etiologia, celularidade e de contagem bacteriana em 10 propriedades leiteiras, localizadas no Estado de São Paulo. Foram examinadas 1148 vacas em lactação, totalizando 4584 glândulas mamárias. Foram considerados os casos, em que houve isolamento de estafilococos coagulase positiva (SCP e estafilococos coagulase negativa (SCN. Os resultados revelaram microbiota com vários patógenos e diferentes espécies de SCN (128 casos e SCP (45, Staphylococcus aureus(90, Streptococcus agalactiae(70, Streptococcus dysgalactiae (69, Streptococcus uberis(29, Corynebacteriumspp. (230, Klebsiella pneumoniae(28, Klebsiella oxytoca(2, Escherichia coli(15, Enterobactersp. (3. Os resultados de contagem de células somáticas (CCS relacionados aos SCP e SCN não mostraram diferenças entre as propriedades avaliadas, entretanto com diferenças significantes ao se avaliar a CCS entre os dois grupos de estafilococos, como pode ser evidenciado ao comparar SCN Discreto e SCP exuberante (P<0,01, SCP Discreto e SCP exuberante (P<0,001 e SCN moderado e SCP exuberante (P<0,01. A avaliação da CCS relacionada à intensidade da infecção, considerando-se como crescimento discreto o isolamento de até nove colônias, moderado de dez a 29 colônias e exuberante, com 30 ou mais colônias, revelou para ambos os grupos de estafilococos que quanto maior o número de unidades formadoras de colônias (UFC, a CCS é mais elevada, sendo sempre maior nos casos de SCP. Conclui-se que quando há maior número de UFC, há concomitantemente maior CCS/mL de leite, no caso dos SCP e SCN, o que mostra relação direta da intensidade do processo infeccioso com a resposta da celularidade do leite, bem como pela relevância desses na etiologia das mastites e dos aspectos

Analysis of four families with the Stickler syndrome by linkage studies. Identification of a new premature stop codon in the COL2A1 gene in a family

Energy Technology Data Exchange (ETDEWEB)

Bonaventure, J.; Lasselin, C. [Hopital Necker, Paris (France); Toutain, A. [CHU Bretonneau, Tours (France)] [and others

1994-09-01

The Stickler syndrome is an arthro-ophthalmopathy which associates progressive myopia with vitreal degeneration and retinal detachment. Cleft palate, cranio-facial abnormalities, deafness and osteoarthritis are often associated symptoms. Genetic heterogeneity of this autosomal dominant disease was consistent with its large clinical variability. Linkage studies have provided evidence for cosegregation of the disease with COL2A1, the gene coding for type II collagen, in about 50% of the families. Four additional families are reported here. Linkage analyses by using a VNTR located in the 3{prime} region of the gene were achieved. In three families, positive lod scores were obtained with a cumulative maximal value of 3.5 at a recombination fraction of 0. In one of these families, single strand conformation analysis of 25 exons disclosed a new mutation in exon 42. Codon for glutamic acid at position a1-803 was converted into a stop codon. The mutation was detected in DNA samples from all the affected members of the family but not in the unaffected. This result confirms that most of the Stickler syndromes linked to COL2A1 are due to premature stop codons. In a second family, an abnormal SSCP pattern of exon 34 was detected in all the affected individuals. The mutation is likely to correspond to a splicing defect in the acceptor site of intron 33. In one family the disease did not segregate with the COL2A1 locus. Further linkage studies with intragenic dimorphic sites in the COL10A1 gene and highly polymorphic markers close to the COL9A1 locus indicated that this disorder did not result from defects in these two genes.

Identification and characterization of the human type II collagen gene (COL2A1).

OpenAIRE

Cheah, Kathryn; Stoker, N.G.; Griffin, J.R.; Grosveld, Frank; Solomon, E.

1985-01-01

textabstractThe gene contained in the human cosmid clone CosHcol1, previously designated an alpha 1(I) collagen-like gene, has now been identified. CosHcol1 hybridizes strongly to a single 5.9-kilobase mRNA species present only in tissue in which type II collagen is expressed. DNA sequence analysis shows that this clone is highly homologous to the chicken alpha 1(II) collagen gene. These data together suggest that CosHcol1 contains the human alpha 1(II) collagen gene COL2A1. The clone appears...

Técnica da instilação de colírios em pacientes portadores de glaucoma crônico Eye drop instillation technique in chronic glaucoma patients

Directory of Open Access Journals (Sweden)

Paulo Gelman Vaidergorn

2003-12-01

Full Text Available OBJETIVO: Verificar a técnica da instilação de colírio em pacientes portadores de glaucoma crônico. MÉTODOS: Estudo prospectivo realizado em 193 pacientes glaucomatosos. Para cada participante era entregue um frasco de colírio lubrificante (Dunason®, Laboratório Alcon, São Paulo, Brasil e solicitado que realizasse uma instilação. RESULTADOS: Os participantes utilizaram, em média, 1,64 ± 1,26 gotas de colírio por instilação e 54,5% dos pacientes fizeram contato do bico do colírio com a superfície ocular. Em 3,1% das instilações nenhuma gota de medicamento atingiu o olho, com o paciente não se dando conta do fato. A oclusão do ponto lacrimal ou a manutenção do olho fechado por dois minutos após a instilação não foi realizada em 87,0% dos participantes, e 61,6% piscaram repetidas vezes imediatamente após instilar a droga. CONCLUSÕES: Verificou-se que a maior parte dos participantes deste estudo efetuou a instilação do colírio de modo incorreto. Isto significa desperdiçar grande parte do conteúdo do frasco, aumentar as possibilidades de toxicidade sistêmica, não aproveitar a plenitude do efeito hipotensor das drogas e contaminar a extremidade do frasco de colírio. Portanto, o ensino da técnica adequada da instilação de colírio é absolutamente necessária para todos os pacientes.PURPOSE: To observe eye drop instillation technique in chronic glaucoma patients. METHODS: This is a prospective study enrolling 193 glaucomatous patients. Each received a sample of lubricant eye drop bottle (Dunason®, Alcon Laboratories, São Paulo, Brazil and was instructed to use it once. RESULTS: All patients used a mean amount of 1.64 ± 1.26 drops. In 54.5% of the patients, the eyedropper touched the eye and adnexa. In 3.1% the drops were not placed in the eye, without the patient being aware of this. In 87.0% of them, neither the lacrimal point occlusion nor the closure of the eyes for two minutes after instillation were

Dual reporter transgene driven by 2.3Col1a1 promoter is active in differentiated osteoblasts

Science.gov (United States)

Marijanovic, Inga; Jiang, Xi; Kronenberg, Mark S.; Stover, Mary Louise; Erceg, Ivana; Lichtler, Alexander C.; Rowe, David W.

2003-01-01

AIM: As quantitative and spatial analyses of promoter reporter constructs are not easily performed in intact bone, we designed a reporter gene specific to bone, which could be analyzed both visually and quantitatively by using chloramphenicol acetyltransferase (CAT) and a cyan version of green fluorescent protein (GFPcyan), driven by a 2.3-kb fragment of the rat collagen promoter (Col2.3). METHODS: The construct Col2.3CATiresGFPcyan was used for generating transgenic mice. Quantitative measurement of promoter activity was performed by CAT analysis of different tissues derived from transgenic animals; localization was performed by visualized GFP in frozen bone sections. To assess transgene expression during in vitro differentiation, marrow stromal cell and neonatal calvarial osteoblast cultures were analyzed for CAT and GFP activity. RESULTS: In mice, CAT activity was detected in the calvaria, long bone, teeth, and tendon, whereas histology showed that GFP expression was limited to osteoblasts and osteocytes. In cell culture, increased activity of CAT correlated with increased differentiation, and GFP activity was restricted to mineralized nodules. CONCLUSION: The concept of a dual reporter allows a simultaneous visual and quantitative analysis of transgene activity in bone.

75 FR 71139 - Land Acquisitions; Puyallup Tribe of Washington

Science.gov (United States)

2010-11-22

... Auditor, in Pierce County, Washington. Except that portion of Lot 3 conveyed to the State of Washington by Deeds recorded under Auditor's file number 689865 and 689858. Together with the East half of the... amendment of Short Plat Nos. 8502210395 and 8403080186, filed with the Pierce County Auditor, in Pierce...

Caracterização de colágenos tipos I e III no estroma do carcinoma de células escamosas cutâneo em cães

Directory of Open Access Journals (Sweden)

S.A.O. Bedoya

2016-02-01

Full Text Available O carcinoma de células escamosas (CCE é uma neoplasia epitelial maligna que acomete cães e diversas outras espécies, incluindo a humana. O CCE afeta vários sítios anatômicos e pode desenvolver metástase. O objetivo deste estudo foi a caracterização das fibras de colágenos tipos I e III no estroma do CCE cutâneo de cães. Para este trabalho, utilizaram-se 44 amostras de pele incluídas em parafina e que tiveram prévio diagnóstico de CCE. As amostras foram processadas histologicamente e coradas com hematoxilina/eosina para confirmação do diagnóstico e classificação do grau de diferenciação tumoral e com a coloração histoquímica de picrosirius para observação dos colágenos tipos I e III. O colágeno tipo III mostrou maior expressão nos CCEs cutâneos bem diferenciados. O papel do colágeno do tipo III nas neoplasias não está bem esclarecido, e outros fatores além do grau de diferenciação celular podem estar envolvidos em sua expressão e determinar sua importância na biologia tumoral.

A novel COL11A1 mutation affecting splicing in a patient with Stickler syndrome.

Science.gov (United States)

Kohmoto, Tomohiro; Naruto, Takuya; Kobayashi, Haruka; Watanabe, Miki; Okamoto, Nana; Masuda, Kiyoshi; Imoto, Issei; Okamoto, Nobuhiko

2015-01-01

Stickler syndrome is a clinically and genetically heterogeneous collagenopathy characterized by ocular, auditory, skeletal and orofacial abnormalities, commonly occurring as an autosomal dominant trait. We conducted target resequencing to analyze candidate genes associated with known clinical phenotypes from a 4-year-old girl with Stickler syndrome. We detected a novel heterozygous intronic mutation (NM_001854.3:c.3168+5G>A) in COL11A1 that may impair splicing, which was suggested by in silico prediction and a minigene assay.

Educator Supply and Demand in Washington State. 2004 Report

Science.gov (United States)

Lashway, Larry; Maloney, Rick; Hathaway, Randy; Bryant, B. J.

2005-01-01

This report describes the findings of the third Educator Supply and Demand Research study in the State of Washington. The intent of these Washington studies is to provide data to inform and shape decisions and activities in the following ways: (1) Provide useful information for educational policymakers, including the legislature, the State Board…

Trends and determinants of cycling in the Washington, DC region.

Science.gov (United States)

2011-01-01

This report analyzes cycling trends, policies, and commuting in the Washington, DC area. The analysis is divided into two parts. : Part 1 focuses on cycling trends and policies in Washington (DC), Alexandria (VA), Arlington County (VA), Fairfax Count...

Polymorphisms within the COL5A1 gene and regulators of the extracellular matrix modify the risk of Achilles tendon pathology in a British case-control study.

Science.gov (United States)

Brown, Karryn L; Seale, Kirsten B; El Khoury, Louis Y; Posthumus, Michael; Ribbans, William J; Raleigh, Stuart M; Collins, Malcolm; September, Alison V

2017-08-01

Several genetic loci have been associated with risk of Achilles tendon pathology (ATP) within South African and Australian populations. The aim of this study was, therefore, to evaluate eight previously implicated genetic variants in an independent British population. A total of 130 asymptomatic controls (CON) and 112 participants clinically diagnosed with ATP comprising 87 individuals with chronic Achilles tendinopathy (TEN) and 25 with Achilles tendon ruptures (RUP) were included. All participants were genotyped for variants within the COL5A1, MIR608, IL-1β, IL-6 and CASP8 genes. Primary findings implicated COL5A1 and CASP8. Three inferred allele combinations constructed from COL5A1 rs12722, rs3196378 and rs71746744 were identified as risk modifiers. The T-C-D combination was associated with increased risk of ATP (P = 0.023) and RUP (P RUP (P = 0.011) and the C-C-D combination was associated with decreased risk of ATP (P = 0.011) and RUP (P = 0.004). The CASP8 rs3834129 DD genotype was associated with decreased risk of TEN (P = 0.020, odds ratio: 0.45, 95% confidence interval: 0.22-0.90) and the CASP8 I-G (rs3834129-rs1045485) inferred allele combination was associated with increased risk of TEN (P = 0.031). This study further highlights the importance of polymorphisms within COL5A1 and CASP8 in the aetiology of ATP.

Alternative Fuels for Washington's School Buses: A Report to the Washington State Legislature.

Science.gov (United States)

Lyons, John Kim; McCoy, Gilbert A.

This document presents findings of a study that evaluated the use of both propane and compressed natural gas as alternative fuels for Washington State school buses. It discusses air quality improvement actions by state- and federal-level regulators and summarizes vehicle design, development, and commercialization activities by all major engine,…

Repositioning Warts & All: A Response to Coteaching Researchers

Directory of Open Access Journals (Sweden)

Jennifer Gallo-Fox

2006-09-01

Full Text Available This paper is a response to our colleague's perspectives on our paper (GALLOFOX, WASSELL, SCANTLEBURY, & JUCK, 2006, that addressed ethical dilemmas we encountered when implementing coteaching in a secondary science education program. Although the respondents addressed this issue, they also raised other important points pertaining to their own experiences with implementing and researching coteaching. In this paper, we synthesize these perspectives and further discuss the implications of implementing coteaching. URN: urn:nbn:de:0114-fqs0604239

77 FR 11582 - Notice of Inventory Completion: Central Washington University Department of Anthropology...

Science.gov (United States)

2012-02-27

...: Central Washington University Department of Anthropology, Ellensburg, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Central Washington University Department of Anthropology has... Central Washington University Department of Anthropology. Repatriation of the human remains and associated...

Residential Energy Efficiency Potential: Washington

Energy Technology Data Exchange (ETDEWEB)

Wilson, Eric J [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

2017-11-27

Energy used by Washington single-family homes that can be saved through cost-effective improvements. Prepared by Eric Wilson and Noel Merket, NREL, and Erin Boyd, U.S. Department of Energy Office of Energy Policy and Systems Analysis.

Results of the Washington Passive Solar Design/Build Competition

Energy Technology Data Exchange (ETDEWEB)

Nylen, N.

1981-01-01

In an effort to encourage the design, construction, and marketing of moderately priced passive solar homes in Washington state, the Western Solar Utilization Network (Western SUN) recently sponsored the Washington Passive Solar Design/Build Competition. The competition drew an overwhelming response from designers and builders throughout Washington. Thermal performance of the designs was evaluated by a technical review committee, and final selections were made by the Competition Jury in accordance with the following criteria: perceived market acceptance, thermal performance, cost effectiveness, simplicity of design and operation, and completeness of the passive concept. Design contract awards totaling $50,000 were made available to winners in four categories, including single and multi-family, new and remodeled residences. In order to receive the award in its entirety, winning design/build teams are required to construct their design by April, 1983. As a result of the competition, a great deal was learned about the attitudes and knowledge of professionals and the general public regarding the use of solar energy in Washington state. Among the points that will be highlighted in this paper are the following: (1) a design/build competition is an effective vehicle for promoting solar energy among professionals in the housing community as well as the general public; (2) passive solar techniques can contribute significantly to the heating and cooling needs of residential housing throughout the state of Washington; (3) there is a great deal of interest and talent among the designers and builders of solar residences in Washington; and (4) follow-up activities, including the promotion of winning designs, the systematic collection of performance data, and identification of the major obstacles confronting designers and builders of solar homes, are critical to the success of the program in achieving both its short-term and long-term goals.

77 FR 15802 - Notice of Inventory Completion: Central Washington University Department of Anthropology...

Science.gov (United States)

2012-03-16

...: Central Washington University Department of Anthropology, Ellensburg, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Central Washington University Department of Anthropology has... contact the Central Washington University Department of Anthropology. Repatriation of the human remains to...

75 FR 52023 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2010-08-24

... DEPARTMENT OF THE INTERIOR National Park Service Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service... of the Thomas Burke Memorial Washington State Museum (Burke Museum), University of Washington...

75 FR 36672 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2010-06-28

... DEPARTMENT OF THE INTERIOR National Park Service Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service... of the Thomas Burke Memorial Washington State Museum (Burke Museum), University of Washington...

Washington State Briefing Book for low-level radioactive waste management

Energy Technology Data Exchange (ETDEWEB)

1980-12-01

The Washington State Briefing Book is one of a series of state briefing books on low-level radioactive waste management practices. It has been prepared to assist state and federal agency officials in planning for safe low-level radioactive waste disposal. The report contains a profile of low-level radioactive waste generators in Washington. The profile is the result of a survey of NRC licensees in Washington. The briefing book also contains a comprehensive assessment of low-level radioactive waste management issues and concerns as defined by all major interested parties including industry, government, the media, and interest groups. The assessment was developed through personal communications with representatives of interested parties, and through a review of media sources. Lastly, the briefing book provides demographic and socioeconomic data and a discussion of relevant government agencies and activities, all of which may impact waste management practices in Washington.

Washington State Briefing Book for low-level radioactive waste management

International Nuclear Information System (INIS)

1980-12-01

The Washington State Briefing Book is one of a series of state briefing books on low-level radioactive waste management practices. It has been prepared to assist state and federal agency officials in planning for safe low-level radioactive waste disposal. The report contains a profile of low-level radioactive waste generators in Washington. The profile is the result of a survey of NRC licensees in Washington. The briefing book also contains a comprehensive assessment of low-level radioactive waste management issues and concerns as defined by all major interested parties including industry, government, the media, and interest groups. The assessment was developed through personal communications with representatives of interested parties, and through a review of media sources. Lastly, the briefing book provides demographic and socioeconomic data and a discussion of relevant government agencies and activities, all of which may impact waste management practices in Washington

77 FR 51564 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2012-08-24

... Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington... of human remains under the control of the Thomas Burke Memorial Washington State Museum (Burke Museum...

77 FR 50157 - Notice of Public Meeting, Eastern Washington Resource Advisory Council Meeting

Science.gov (United States)

2012-08-20

...; HAG 12-0260] Notice of Public Meeting, Eastern Washington Resource Advisory Council Meeting AGENCY.... Department of the Interior, Bureau of Land Management (BLM) Eastern Washington Resource Advisory Council... Bureau of Land Management's Eastern Washington and San Juan Resource Management Plan and the U.S. Forest...

77 FR 23495 - Notice of Public Meeting, Eastern Washington Resource Advisory Council Meeting

Science.gov (United States)

2012-04-19

...; HAG 12-0164] Notice of Public Meeting, Eastern Washington Resource Advisory Council Meeting AGENCY... 1972, the U.S. Department of the Interior, Bureau of Land Management (BLM) Eastern Washington Resource... of Land Management's Eastern Washington and San Juan Resource Management Plan, and the U.S. Forest...

Heat-related illness in Washington State agriculture and forestry sectors.

Science.gov (United States)

Spector, June T; Krenz, Jennifer; Rauser, Edmund; Bonauto, David K

2014-08-01

We sought to describe heat-related illness (HRI) in agriculture and forestry workers in Washington State. Demographic and clinical Washington State Fund workers' compensation agriculture and forestry HRI claims data (1995-2009) and Washington Agriculture Heat Rule citations (2009-2012) were accessed and described. Maximum daily temperature (Tmax) and Heat Index (HImax) were estimated by claim date and location using AgWeatherNet's weather station network. There were 84 Washington State Fund agriculture and forestry HRI claims and 60 Heat Rule citations during the study period. HRI claims and citations were most common in crop production and support subsectors. The mean Tmax (HImax) was 95°F (99°F) for outdoor HRI claims. Potential HRI risk factors and HRI-related injuries were documented for some claims. Agriculture and forestry HRI cases are characterized by potential work-related, environmental, and personal risk factors. Further work is needed to elucidate the relationship between heat exposure and occupational injuries. © 2014 Wiley Periodicals, Inc.

The expanding spectrum of COL2A1 gene variants IN 136 patients with a skeletal dysplasia phenotype.

Science.gov (United States)

Barat-Houari, Mouna; Dumont, Bruno; Fabre, Aurélie; Them, Frédéric Tm; Alembik, Yves; Alessandri, Jean-Luc; Amiel, Jeanne; Audebert, Séverine; Baumann-Morel, Clarisse; Blanchet, Patricia; Bieth, Eric; Brechard, Marie; Busa, Tiffany; Calvas, Patrick; Capri, Yline; Cartault, François; Chassaing, Nicolas; Ciorca, Vidrica; Coubes, Christine; David, Albert; Delezoide, Anne-Lise; Dupin-Deguine, Delphine; El Chehadeh, Salima; Faivre, Laurence; Giuliano, Fabienne; Goldenberg, Alice; Isidor, Bertrand; Jacquemont, Marie-Line; Julia, Sophie; Kaplan, Josseline; Lacombe, Didier; Lebrun, Marine; Marlin, Sandrine; Martin-Coignard, Dominique; Martinovic, Jelena; Masurel, Alice; Melki, Judith; Mozelle-Nivoix, Monique; Nguyen, Karine; Odent, Sylvie; Philip, Nicole; Pinson, Lucile; Plessis, Ghislaine; Quélin, Chloé; Shaeffer, Elise; Sigaudy, Sabine; Thauvin, Christel; Till, Marianne; Touraine, Renaud; Vigneron, Jacqueline; Baujat, Geneviève; Cormier-Daire, Valérie; Le Merrer, Martine; Geneviève, David; Touitou, Isabelle

2016-07-01

Heterozygous COL2A1 variants cause a wide spectrum of skeletal dysplasia termed type II collagenopathies. We assessed the impact of this gene in our French series. A decision tree was applied to select 136 probands (71 Stickler cases, 21 Spondyloepiphyseal dysplasia congenita cases, 11 Kniest dysplasia cases, and 34 other dysplasia cases) before molecular diagnosis by Sanger sequencing. We identified 66 different variants among the 71 positive patients. Among those patients, 18 belonged to multiplex families and 53 were sporadic. Most variants (38/44, 86%) were located in the triple helical domain of the collagen chain and glycine substitutions were mainly observed in severe phenotypes, whereas arginine to cysteine changes were more often encountered in moderate phenotypes. This series of skeletal dysplasia is one of the largest reported so far, adding 44 novel variants (15%) to published data. We have confirmed that about half of our Stickler patients (46%) carried a COL2A1 variant, and that the molecular spectrum was different across the phenotypes. To further address the question of genotype-phenotype correlation, we plan to screen our patients for other candidate genes using a targeted next-generation sequencing approach.

Reaching Higher. A Parent's Guide to the Washington Assessment of Learning. Revised = Para llegar mas arriba. Una guia para padres sobre la evaluacion del aprendizaje de los estudiantes del estado de Washington (Washington Assessment of Student Learning). Revisado.

Science.gov (United States)

Washington Office of the State Superintendent of Public Instruction, Olympia.

This guide in English and Spanish is designed to answer questions parents may have about the Washington Assessment of Student Learning (WASL), including how it will help improve their children's education, how it is scored, and how to use the information it provides. In Washington, clear educational goals for subject content, thinking skills, and…

Racismo y derechos humanos en Colombia: avatares de las poblaciones afrocolombianas; Racismo e direitos humanos na Colômbia: avatares das populações afro- colombianas; Racism and human rights in Colombia: avatars of the Afro-Colombian peoples

Directory of Open Access Journals (Sweden)

Adriana Espinosa Bonilla

2015-07-01

Full Text Available Resumen: Este artículo pretende plantear una reflexión acerca de la forma en que se ha configurado el racismo frente a las poblaciones negras en Colombia y cómo las luchas en el campo político del movimiento social afrocolombiano, han esgrimido el discurso de los derechos humanos como estrategia de resistencia. Dicho análisis, describe las interacciones de los representantes del gobierno con los activistas de las organizaciones negras, en el escenario de las audiencias ante la Comisión Interamericana de Derechos Humanos en Washington, donde aparecen los discursos relacionados de racismo y derechos humanos, en la contienda entre representantes del estado y comunidades negras.   Palabras clave: Racismo, Derechos Humanos, ciudadanía, Identidad, Movimiento Social, Organizaciones Negras.       Resumo: Este artigo pretende expor uma reflexão sobre a forma como se configurou o racismo frente as populações negras na Colômbia e como as lutas no campo político do movimento social afro-colombiano brandiu o discurso dos direitos humanos como estratégia de resistência. Essa analise descreve as interações dos representantes do governo com os ativistas das organizações negras no cenário das audiências na Corte Interamericana de Direitos Humanos, em Washington, onde aparecem discursos relacionados com o racismo e os direitos humanos, nas disputas entre representantes do Estado e das comunidades negras.   Palavras-chave: Racismo, DIreitos Humanos, cidadania, identidade, Movimento social, Organizações Negras.       Abstract: This article aims at presenting a reflection on how racism against black populations in Colombia has been configured and how the fights in the political     field of the Afro-Colombian social movement have employed the discourse of human rights as a strategy of resistance. Such analysis describes the interactions between the government representatives and the activists of black organizations on the stage of the

A crise Armada Colômbia-Equador no contexto da Guerra contra o Terrorismo Internacional

Directory of Open Access Journals (Sweden)

TATIANA WAISBERG

2010-10-01

Full Text Available A recente crise armada entre Colômbia e Equador trouxe a América do Sul ao foco da mídia internacional. As FARC representam o maior barril de pólvora da América Latina, além de um determinante ator a ser combatido na luta contra o terrorismo internacional. Nesse sentido, o presente artigo busca analisar, sob o prisma da segurança e do direito internacional o papel das FARC em âmbito global e as maneiras segundo as quais elas são combatidas.

Marijuana, other drugs, and alcohol use by drivers in Washington State.

Science.gov (United States)

2016-07-01

In Washington State legal sales of marijuana began July 8, 2014. A voluntary, anonymous roadside study was conducted to assess the prevalence of drivers testing positive for alcohol and other drugs, including marijuana, on Washingtons roads. Data ...

Southwestern Washington 6 arc-second DEM

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The 6-second Southwest Washington Elevation Grid provides bathymetric data in ASCII raster format of 6-second resolution in geographic coordinates. This grid is...

77 FR 37317 - Drawbridge Operation Regulation; Lake Washington, Seattle, WA

Science.gov (United States)

2012-06-21

..., 2012. The deviation allows the floating draw span of the SR 520 Lake Washington Bridge to remain in the... schedule that governs the State Route 520 (SR 520) Bridge across Lake Washington at Seattle, WA. This... allows the bridge to remain in the closed position to allow safe movement of event participants. DATES...

Taxa de Infestação da Varroa destructor em colônias de Apis mellifera L. no Agreste Meridional de Pernambuco

Directory of Open Access Journals (Sweden)

Deygnon Cavalcanti Clementino

2016-08-01

Full Text Available O presente trabalho teve como objetivo verificar a taxa de infestação da Varroa destructor em colônias de Apis mellifera L. proveniente de apiário no município de Lagoa do Ouro, Agreste Meridional de Pernambuco. A pesquisa foi realizada no mês de setembro de 2015. Participaram do estudo 15 colônias, em que foram coletadas em média 227 abelhas adultas de cada colmeia e acondicionadas em recipientes contendo álcool a 70%. Após as coletas a campo, as amostras foram transportadas para o laboratório de Biologia Animal da Universidade Federal Rural de Pernambuco/Unidade Acadêmica de Garanhuns, para a realização das análises e quantificação dos ácaros. Os resultados demonstraram que 100% das amostras estavam parasitadas com o ácaro, sendo que a média geral da taxa de infestação foi de 6,16%. O grau de infestação pela Varroa destructor observado nas colônias variou de 0,93% a 11,15%. Conclui-se que as colônias analisadas apresentam diferentes níveis de infestação pelo ácaro Varroa destructor.Infestation rate of Varroa destructor in Apis mellifera L. colonies in the South Agreste of PernambucoAbstract: This study aimed to verify the infestation rate of Varroa destructor in Apis mellifera L. colonies in the apiary from Lagoa do Ouro County, South Agreste of Pernambuco. The survey was conducted in September 2015. The study included 15 colonies, which were collected on average 227 adult bees in each hive and placed in vials containing 70% alcohol. After harvesting the field, the samples were transported to the Animal Biology Laboratory at the Federal Rural University of Pernambuco/Academic Unit of Garanhuns, to perform the analysis and quantification of mites. The results showed that 100% of the samples were infested with mites, and the overall average infestation rate was 6.16%. The degree of infestation by Varroa destructor observed in colonies ranged from 0.93% to 11.15%. It is concluded that the analyzed colonies have

Timber resource statistics for southwest Washington.

Science.gov (United States)

Patricia M. Bassett; Daniel D. Oswald

1981-01-01

This report summarizes a 1978 timber-resource inventory of six counties in southwest Washington: Clark, Cowlitz, Lewis, Pacific, Skamania, and Wahkiakum. Detailed tables of forest area, timber volume, growth, mortality, and harvest are presented.

A novel zinc finger protein 219-like (ZNF219L) is involved in the regulation of collagen type 2 alpha 1a (col2a1a) gene expression in zebrafish notochord.

Science.gov (United States)

Lien, Huang-Wei; Yang, Chung-Hsiang; Cheng, Chia-Hsiung; Hung, Chin-Chun; Liao, Wei-Hao; Hwang, Pung-Pung; Han, Yu-San; Huang, Chang-Jen

2013-01-01

The notochord is required for body plan patterning in vertebrates, and defects in notochord development during embryogenesis can lead to diseases affecting the adult. It is therefore important to elucidate the gene regulatory mechanism underlying notochord formation. In this study, we cloned the zebrafish zinc finger 219-like (ZNF219L) based on mammalian ZNF219, which contains nine C2H2-type zinc finger domains. Through whole-mount in situ hybridization, we found that znf219L mRNA is mainly expressed in the zebrafish midbrain-hindbrain boundary, hindbrain, and notochord during development. The znf219L morpholino knockdown caused partial abnormal notochord phenotype and reduced expression of endogenous col2a1a in the notochord specifically. In addition, ZNF219L could recognize binding sites with GGGGG motifs and trigger augmented activity of the col2a1a promoter in a luciferase assay. Furthermore, in vitro binding experiments revealed that ZNF219L recognizes the GGGGG motifs in the promoter region of the zebrafish col2a1a gene through its sixth and ninth zinc finger domains. Taken together, our results reveal that ZNF219L is involved in regulating the expression of col2a1a in zebrafish notochord specifically.

To Be a Slave: The Boyhood of Booker T. Washington.

Science.gov (United States)

National Park Service (Dept. of Interior), Washington, DC.

The Booker T. Washington National Monument preserves and protects the birth site and childhood home of Booker T. Washington, while interpreting his life experiences and significance in U.S. history as the most powerful African American between 1895 and 1915. The park provides a resource for public education and a focal point for continuing…

O berço francês na nossa educação: do projeto do Colégio Pedro II ao Bac-ENEM

Directory of Open Access Journals (Sweden)

Cynthia Agra de Brito Neves

2015-12-01

Full Text Available Este artigo tem por finalidade refletir sobre as raízes e os vínculos transversais do Brasil com a França a partir da investigação das heranças e influências da pedagogia francesa na formação do nosso ensino secundário, sobretudo em meados do século XIX, quando o Colégio Pedro II, a escola modelo, era referência nacional e importava currículos e programas de estudos dos lycées franceses. Almeida (2000, Doria (1997, Santos (2003, Vechia e Cavazotti et al (2003 e, principalmente, Razzini (2000 foram contribuições preciosas para constatarmos o papel das ideias libertárias e literárias dessa pedagogia na excelência desse Colégio no período do Império à República no Brasil. Criado em 1837, sob tutela do Estado e com o nome do imperador, o Colégio estabelecia um projeto de nação baseado nessas ideias. A instituição – responsável pela formação de nomes como Joaquim Manuel de Macedo, Manuel Bandeira e Pedro Nava – contava com um corpo docente de prestígio que se dedicou, durante décadas, à formação dos filhos da elite brasileira que procurava naquela escola uma educação inspirada no modelo clássico e humanista francês.

Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome.

Science.gov (United States)

Chiarelli, Nicola; Carini, Giulia; Zoppi, Nicoletta; Ritelli, Marco; Colombi, Marina

2018-01-01

Vascular Ehlers-Danlos syndrome (vEDS) is a dominantly inherited connective tissue disorder caused by mutations in the COL3A1 gene that encodes type III collagen (COLLIII), which is the major expressed collagen in blood vessels and hollow organs. The majority of disease-causing variants in COL3A1 are glycine substitutions and in-frame splice mutations in the triple helix domain that through a dominant negative effect are associated with the severe clinical spectrum potentially lethal of vEDS, characterized by fragility of soft connective tissues with arterial and organ ruptures. To shed lights into molecular mechanisms underlying vEDS, we performed gene expression profiling in cultured skin fibroblasts from three patients with different structural COL3A1 mutations. Transcriptome analysis revealed significant changes in the expression levels of several genes involved in maintenance of cell redox and endoplasmic reticulum (ER) homeostasis, COLLs folding and extracellular matrix (ECM) organization, formation of the proteasome complex, and cell cycle regulation. Protein analyses showed that aberrant COLLIII expression is associated with the disassembly of many structural ECM constituents, such as fibrillins, EMILINs, and elastin, as well as with the reduction of the proteoglycans perlecan, decorin, and versican, all playing an important role in the vascular system. Furthermore, the altered distribution of the ER marker protein disulfide isomerase PDI and the strong reduction of the COLLs-modifying enzyme FKBP22 are consistent with the disturbance of ER-related homeostasis and COLLs biosynthesis and post-translational modifications, indicated by microarray analysis. Our findings add new insights into the pathophysiology of this severe vascular disorder, since they provide a picture of the gene expression changes in vEDS skin fibroblasts and highlight that dominant negative mutations in COL3A1 also affect post-translational modifications and deposition into the ECM of

Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome.

Directory of Open Access Journals (Sweden)

Nicola Chiarelli

Full Text Available Vascular Ehlers-Danlos syndrome (vEDS is a dominantly inherited connective tissue disorder caused by mutations in the COL3A1 gene that encodes type III collagen (COLLIII, which is the major expressed collagen in blood vessels and hollow organs. The majority of disease-causing variants in COL3A1 are glycine substitutions and in-frame splice mutations in the triple helix domain that through a dominant negative effect are associated with the severe clinical spectrum potentially lethal of vEDS, characterized by fragility of soft connective tissues with arterial and organ ruptures. To shed lights into molecular mechanisms underlying vEDS, we performed gene expression profiling in cultured skin fibroblasts from three patients with different structural COL3A1 mutations. Transcriptome analysis revealed significant changes in the expression levels of several genes involved in maintenance of cell redox and endoplasmic reticulum (ER homeostasis, COLLs folding and extracellular matrix (ECM organization, formation of the proteasome complex, and cell cycle regulation. Protein analyses showed that aberrant COLLIII expression is associated with the disassembly of many structural ECM constituents, such as fibrillins, EMILINs, and elastin, as well as with the reduction of the proteoglycans perlecan, decorin, and versican, all playing an important role in the vascular system. Furthermore, the altered distribution of the ER marker protein disulfide isomerase PDI and the strong reduction of the COLLs-modifying enzyme FKBP22 are consistent with the disturbance of ER-related homeostasis and COLLs biosynthesis and post-translational modifications, indicated by microarray analysis. Our findings add new insights into the pathophysiology of this severe vascular disorder, since they provide a picture of the gene expression changes in vEDS skin fibroblasts and highlight that dominant negative mutations in COL3A1 also affect post-translational modifications and deposition

Increased Frequency of ColV Plasmids and Mannose-Resistant Hemagglutinating Activity in an Escherichia coli K1 Population

OpenAIRE

1984-01-01

The expression of traits linked to pathogenicity was studied in a population of Escherichia coli K1 strains. It was found that E. coli K1 strains isolated from extraintestinal infection harbor the ColV plasmid and express mannose-resistant hemagglutinating activity type VI with a high frequency. The presence of these properties may play a role in the ability of some E. coli K1 serogroups to invade.

Timber resource statistics for eastern Washington.

Science.gov (United States)

Patricia M. Bassett; Daniel D. Oswald

1983-01-01

This report summarizes a 1980 timber resource inventory of the 16 forested counties in Washington east of the crest of the Cascade Range. Detailed tables of forest area, timber volume, growth, mortality, and harvest are presented.

Compósito de colágeno com silicato e hidroxiapatita como material para endodontia: preparação e caracterização = Collagen composite with silicate and hydroxyapatite as endodontic material: preparation and characterization

Directory of Open Access Journals (Sweden)

E'Gues, Miguel Antônio Menezes

2008-01-01

Full Text Available Objetivo: Este trabalho teve por objetivo preparar e caracterizar um novo compósito de colágeno com silicato e hidroxiapatita que possa vir a ser utilizado como material endodôntico em capeamento pulpar direto e em pulpotomia. Metodologia: Os materiais utilizados como matéria-prima foram colágeno do tipo I na forma aniônica, silicato (cimento Portland Branco e hidroxiapatita sintetizada. Estes materiais foram misturados em proporções variadas de modo a obter um material compósito na forma de pasta. Os materiais obtidos foram caracterizados por técnicas de calorimetria exploratória diferencial, termogravimetria, microscopia eletrônica de varredura (MEV, espectrometria de dispersão de energia de Raios-X e espectroscopia na região do infravermelho. Resultados: A presença de colágeno no compósito foi demonstrada por transições térmicas típicas daquelas encontradas para o colágeno do tipo I (~48ºC, independentemente da proporção utilizada, e também por imagens de MEV, onde as fibras colagênicas também puderam ser observadas interligando as partículas de silicato. A composição química de todos compósitos de colágeno foi praticamente idêntica à do silicato utilizado. Conclusão: Os resultados mostraram que a estrutura da matriz de colágeno nos compósitos testados não foi alterada. Esta é uma característica importante para a utilização em endodontia do material compósito proposto, objetivando uma reparação tecidual de forma "mais natural"

77 FR 64718 - Safety Zone; Steam Ship Col. James M. Schoonmaker Relocation Project, Maumee River, Toledo, OH

Science.gov (United States)

2012-10-23

...The Coast Guard is establishing a temporary Safety Zone on the waters of Maumee River, Toledo, Ohio, in support of the relocation of S/S Col. James M. Schoonmaker (hereinafter referred to as `the Schoonmaker'). This temporary safety zone will encompass all waters of the Maumee River within a 100 yard radius of the Schoonmaker as it makes its transit from International Park to Skyway Marina. This temporary Safety Zone is necessary to protect persons operating around the area.

Sigma Theta Tau, DAaL/Texas Nurse Association, 2017 South Texas Nurse Imagemaker, Lt Col Jacqueline Killian

Science.gov (United States)

2018-04-28

ENDORSEMENT (59 MDW/SGVU Use Only) 43. REVIEWER SIGNATURE 32. DATE 38. DATE 44. DATE April 11 , 20 17 45. DATE RECEIVED 1 46. SENIOR AUTHOR NOTIFIED BY...EBP mentors to facilitate the growing number of EBP projects underway in our organization. 1 Lt Col Killian serves as research consultant to chief...for helping others. The first project is focused on enhancing resil ience in our military families--the " Mentors Offering Maternal Support (M-0-M

40 CFR 81.348 - Washington.

Science.gov (United States)

2010-07-01

... 5/14/01 Attainment The City of Kent and a portion of the Green River valley bounded on the east and... Type Seattle-Tacoma Area: Seattle-Tacoma Urban Area (as defined by the Washington Department of Transportation urban area maps) King County (part) Attainment Pierce County (part) Attainment Snohomish County...

Géis de colágeno aniônico: ransana como biomateriais. Preparação e caracterização físico-química

OpenAIRE

Goissis,Gilberto; Góes,Júlio C.

1997-01-01

Este trabalho descreve o efeito da ransana, um polissacarídeo bacteriano, sobre géis de colágeno aniônico. A interação colágeno:ransana ocorreu independentemente do pH, mesmo com baixas concentrações de ransana, e os materiais obtidos no estado sólido foram caracterizados por serem mais estáveis térmicamente à medida em que se aumenta a concentração do polissacarídeo. Nenhuma alteração na estrutura secundária em tripla hélice do tropocolágeno foi observada. O efeito mais significativo da rans...

Refining aging criteria for northern sea otters in Washington State

Science.gov (United States)

Schuler, Krysten L.; Baker, Bridget B.; Mayer, Karl A.; Perez-Heydrich, Carolina; Holahan, Paula M.; Thomas, Nancy J.; White, C. LeAnn

2018-01-01

Measurement of skull ossification patterns is a standard method for aging various mammalian species and has been used to age Russian, Californian, and Alaskan sea otter populations. Cementum annuli counts have also been verified as an accurate aging method for the Alaskan sea otter population. In this study, cementum annuli count results and skull ossification patterns were compared as methods for aging the northern sea otter (Enhydra lutris kenyoni) population in Washington State. Significant agreement was found between the two methods suggesting that either method could be used to age the Washington population of otters. This study also found that ossification of the squamosal-jugal suture at the ventral glenoid fossa can be used to differentiate male subadults from adults. To assist field biologists or others without access to cementum annuli or skull ossification analysis techniques, a suite of morphologic, physiologic, and developmental characteristics were analyzed to assess whether a set of these more easily accessible parameters could also predict age class for the Washington population of otters. Tooth condition score, evidence of reproductive activity in females, and tooth eruption pattern were identified as the most useful criteria for classifying Washington sea otters as pups, juveniles, subadults, or adults/aged adults. A simple decision tree based on characteristics accessible in the field or at necropsy was created that can be used to reliably predict age class of Washington sea otters as determined by cementum annuli.

76 FR 18001 - Irish Potatoes Grown in Washington; Decreased Assessment Rate

Science.gov (United States)

2011-04-01

... IR] Irish Potatoes Grown in Washington; Decreased Assessment Rate AGENCY: Agricultural Marketing... rate established for the State of Washington Potato Committee (Committee) for the 2011-2012 and subsequent fiscal periods from $0.0035 to $0.003 per hundredweight of potatoes handled. The Committee locally...

76 FR 41589 - Irish Potatoes Grown in Washington; Decreased Assessment Rate

Science.gov (United States)

2011-07-15

... FIR] Irish Potatoes Grown in Washington; Decreased Assessment Rate AGENCY: Agricultural Marketing... established for the State of Washington Potato Committee (Committee) for the 2011-2012 and subsequent fiscal periods from $0.0035 to $0.003 per hundredweight of potatoes handled. The Committee locally administers...

A Partnership for Modeling the Marine Environment of Puget Sound, Washington

Science.gov (United States)

2009-03-30

Northwest National Marine Renewable Energy Center, a joint University of Washington - Oregon State project funded by the U.S. Department of Energy. e. A... Marine Renewable Energy Center (NNMREC), a joint Washington - Oregon State project to investigate extraction of wave and tidal energy sponsored by

Booker T. Washington's Educational Contributions to Contemporary Practices of Sustainable Development

Science.gov (United States)

Grant, Brett G.

2014-01-01

This article discusses Booker T. Washington's educational contributions to contemporary practices of sustainable development. In particular, the article looks at Washington's contributions in the areas of economic sustainability and entrepreneurship, character development, and aesthetics. As states continue to contemplate and evaluate the value of…

Prácticas escolares en el Colégio Americano: un enfoque en la cultura material

Directory of Open Access Journals (Sweden)

Marlucy do Socorro Aragão de Sousa

2017-05-01

Full Text Available Este artículo aborda las prácticas escolares y la cultura material de la escuela en Belém do Pará, a finales del siglo XIX. El objetivo es analizar cómo se configuraron la cultura material y las prácticas escolares en el Colégio Americano, ideado por José Veríssimo entre 1884 y 1890. Se usa como fuente la obra Noticia Geral sobre o Collegio Americano. Se utilizó la metodología histórico-hermenéutica. Al analizar la cultura material de la escuela, se tiene como horizonte la percepción de sus vínculos con el contexto histórico, cultural y social en el que se generó un nuevo modelo de educación que destacaba la civilidad, la nación y la moral. Por consiguiente, dichos artefactos no eran simples objetos, sino que revelaron las relaciones conflictivas o pacíficas de aquel contexto, e incluso las prácticas escolares de la institución. Se observa que el uso y la descripción de los objetos dispuestos en la estructura del Colégio Americano, para su creador, tenían el objetivo de establecer y mantener normas que debían formar hombres disciplinados, que se vincularan directamente con el propósito de la educación física, moral y cívica de los estudiantes.

Estandarización de un proceso de extracción de colágeno a partir de los residuos de fileteo de tilapia (Oreochromis sp) y cachama (Piaractus brachypomus) / Standardization of a process for extracting collagen from filleting waste from tilapia (Oreochromis sp) and cachama (Piaractus brachypomus)

OpenAIRE

Serrano Gaona, Jenifer Carolina

2011-01-01

Se estandarizó un proceso de extracción y purificación de colágeno ácido soluble a partir de pieles de tilapia (Oreochromis sp) y de cachama (Piaractus brachypomus) utilizando análisis estadísticos. Los resultados indicaron que el rendimiento de colágeno ácido soluble fue de 33,3% en base seca. El patrón de alto peso molecular utilizado para SDS – PAGE reveló que el colágeno obtenido de pieles de tilapia o de pieles de cachama es colágeno tipo I. La temperatura de desnaturalización (Td) del c...

Influence of aging on the quality of the skin of white women: the role of collagen Influência do envelhecimento na qualidade da pele de mulheres brancas: o papel do colágeno

Directory of Open Access Journals (Sweden)

Eloina do Rocio Valenga Baroni

2012-10-01

Full Text Available PURPOSE: Evaluate the influence of aging on the quality of the skin of white women, analyzing the dermal collagen. METHODS: Pre-auricular flaps were collected for histological and morphometric analysis of 218 white women who underwent spontaneous facial aesthetic plastic surgery. Picrosirius ultrared stain was used for analysis and quantification of collagen in five age groups (OBJETIVO: Avaliar a influência do envelhecimento na qualidade da pele de mulheres brancas analisando o colágeno dérmico. MÉTODOS: Realizou-se análise histológica e morfométrica de 218 retalhos pré-auriculares de mulheres brancas que se submeteram espontaneamente à cirurgia estética facial. Foi usada a coloração de Picrosirius Ultrared para analisar e quantificar os colágenos I, III e total em cinco grupos etários (<40 anos, 40 a 49 anos, 50 a 59 anos, 60 a 69 anos e 70 a 79 anos. RESULTADOS: A análise histológica mostrou alterações sugestivas de envelhecimento cutâneo (fragmentação e desorganização das fibras de colágeno, especialmente em pacientes acima de 60 anos. Não houve diferenças significantes entre a idade e a espessura da derme e da epiderme, mas houve diferenças significantes entre as percentagens de colágeno I, III e total (p<0,001 com o aumento da idade. CONCLUSÃO: Existe redução do colágeno com o aumento da idade e um aumento na sua degradação, levando à fragmentação das fibras.

An annotated checklist of the vascular flora of Washington County Mississippi

Science.gov (United States)

Field explorations have yielded 257 species new to Washington County, Mississippi and Calandrinia ciliata (Ruiz & Pav.) DC. and Ruellia nudiflora (Engelm. & Gray) Urban new to the state. An annotated list of 796 taxa for Washington County is provided and excludes 62 species that were reported from ...

Washington: a guide to geothermal energy development

Energy Technology Data Exchange (ETDEWEB)

Bloomquist, R.G.; Basescu, N.; Higbee, C.; Justus, D.; Simpson, S.

1980-06-01

Washington's geothermal potential is discussed. The following topics are covered: exploration, drilling, utilization, legal and institutional setting, and economic factors of direct use projects. (MHR)

Xarxes educatives 2.1. Mitjans socials, entorns col·laboratius i processos d'ensenyament i aprenentatge

OpenAIRE

Levis, Diego; Diéguez, Sol; Rey, Eugenia

2011-01-01

El projecte Xarxes Educatives 2.1 es proposa indagar les possibilitats que ofereixen els mitjans socials i els entorns col·laboratius a internet en els processos d'ensenyament i aprenentatge. Per a això, en cursos presencials i en cursos de capacitació docent de modalitat semipresencial fem servir una aplicació de la web 2.0 destinada a la creació de xarxes socials. L'aplicació es va configurar d'acord amb els objectius pedagògics de cada un dels cursos inclosos en el projecte. Es va establir...

78 FR 48285 - Irish Potatoes Grown in Washington; Decreased Assessment Rate

Science.gov (United States)

2013-08-08

...; FV13-946-1 FIR] Irish Potatoes Grown in Washington; Decreased Assessment Rate AGENCY: Agricultural... established for the State of Washington Potato Committee (Committee) for the 2013-2014 fiscal year and all subsequent fiscal periods from $0.003 to $0.0025 per hundredweight of potatoes handled. The Committee locally...

O hormônio de crescimento e a concentração de colágeno na cicatrização de feridas cutâneas de ratos

Directory of Open Access Journals (Sweden)

Biondo-Simões Maria de Lourdes Pessole

2000-01-01

Full Text Available Investiga-se a interferência do hormônio de crescimento no processo de cicatrização cutânea de ratos. Utilizaram-se 50 ratos machos com 170 dias de idade divididos em 2 grupos: controle (n=25 e experimento (n=25. Submeteram-se os ratos dos dois grupos à laparotomia mediana de 4 centímetros sob anestesia inalatória de éter etílico. Realizou-se a laparorrafia com 2 planos de sutura, o primeiro peritônio-músculo-aponevrótico e o segundo o da pele com chuleio contínuo de fio monofilamentar de náilon 4.0. Os animais do grupo experimento receberam 0,4U/kg/dia de hormônio de crescimento por via subcutânea diluído em 0,12 ml de água destilada e os do grupo controle recebiam igual volume do veículo pela mesma via. Realizaram-se as aferições após 36, 72, 168, 240 e 336 horas, ressecando-se retalhos da parede abdominal contendo as cicatrizes, que após preparação histológica e tratamento pelo Sirius red, forneceram a concentração do colágeno. O percentual da área de cicatriz ocupada por colágeno foi maior no grupo experimento nas 36 horas (p=0,0106, 72 horas (p=0,0089, 168 horas (p=0,0149 e 336 horas (p=0,0116. O percentual de colágeno I foi semelhante nos dois grupos nas 36 e 72 horas e maior no grupo experimento nas 168 horas (p=0,0218, 240 horas (p=0,0067 e nas 336 horas (p=0,0027. O percentual de colágeno III apareceu em proporção semelhante nos 2 grupos nas 36 e 72 horas. Sua proporção foi maior no grupo controle nas 168 horas (p=0,0216, 240 horas (p=0,0067 e 336 horas (p=0,0027. Estes dados permitiram concluir que o hormônio de crescimento levou a maior concentração de colágeno e acelerou a maturação das cicatrizes.

Marijuana, other drugs, and alcohol use by drivers in Washington state : appendices.

Science.gov (United States)

2016-07-01

In Washington State legal sales of marijuana began July 8, 2014. A voluntary, anonymous roadside study was conducted to assess the prevalence of drivers testing positive for alcohol and other drugs, including marijuana, on Washingtons roads. Data ...

75 FR 14463 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

Science.gov (United States)

2010-03-25

... Washington, Department of Anthropology, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice... University of Washington, Department of Anthropology, Seattle, WA. The human remains were removed from... University of Washington, Department of Anthropology and Burke Museum staff in consultation with...

76 FR 58033 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2011-09-19

... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum (Burke...

75 FR 36671 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2010-06-28

... DEPARTMENT OF THE INTERIOR National Park Service Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA; Correction AGENCY: National Park... human remains and associated funerary objects in the possession of the Thomas Burke Memorial Washington...

76 FR 58034 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2011-09-19

... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum (Burke...

76 FR 58039 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2011-09-19

... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum (Burke...

Collection Development Policy: Federal Government Publications at Eastern Washington University Libraries.

Science.gov (United States)

Roselle, Ann; Chan, Karen

This collection development policy serves as a guide for the selection and retention of depository government documents by the Government Publications Unit of the Kennedy Memorial Library of Eastern Washington University (EWU) in Cheney, Washington. The library selects approximately 65 percent of the depository items distributed by the U.S.…

The Impact of Interstate Migration on Human Capital Development in Washington

Science.gov (United States)

Spaulding, Randy

2010-01-01

Washington State is a leader in the innovation economy largely due to the combination of aerospace, software, and biomedical industries centered in the greater Seattle area; and, the state's high level of international trade. Despite Washington's national ranking, the state is overly reliant on importing educated workers from other states and…

Washington Schools Learn from Value Engineering.

Science.gov (United States)

Doleae, Michael L.; Childs, Harvey C.

1983-01-01

Results of two value engineering studies have shown that a review early in the design process can help save costs in school construction, maintenance, operation, and replacement. The value engineering concepts and technical manual are being presented throughout the state of Washington. (MLF)

77 FR 46117 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2012-08-02

... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-10823; 2200-1100-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington...

78 FR 11675 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2013-02-19

... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-12080;2200-1100-665] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington...

Integrated Digital English Acceleration (I-DEA). Washington's Community and Technical Colleges

Science.gov (United States)

Washington State Board for Community and Technical Colleges, 2015

2015-01-01

Washington state has a large and rapidly growing foreign-born population. In 2011, immigrants made up 16.5 percent of Washington's civilian employed workforce, up from 7.1 percent in 1990. These new arrivals create jobs by forming businesses, spending income in local economies and raising employers' productivity. Thanks to project I-DEA…

Washington (Wash) C. Winn: In Memoriam

Centers for Disease Control (CDC) Podcasts

2012-03-08

Dr. Mike Miller and Dr. David Walker dicuss the career and life of noted clinical biologist, Dr. Washington C. Winn Jr.  Created: 3/8/2012 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 3/12/2012.

78 FR 64006 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2013-10-25

... inventory of human remains under the control of the Burke Museum. The human remains were removed from Island....R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of... Memorial Washington State Museum, University of Washington (Burke Museum), has completed an inventory of...

78 FR 59955 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2013-09-30

... completion of an inventory of human remains under the control of the Burke Museum, Seattle, WA. The human....R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of... Memorial Washington State Museum, University of Washington (Burke Museum), has completed an inventory of...

NSA Diana Wueger Published in Washington Quarterly

OpenAIRE

Grant, Catherine L.

2016-01-01

National Security Affairs (NSA) News NSA Faculty Associate for Research Diana Wueger has recently had an article titled “India’s Nuclear-Armed Submarines: Deterrence or Danger?” published in the Washington Quarterly.

Bibliography of the geology of the Columbia Basin and surrounding areas of Washington

International Nuclear Information System (INIS)

Tucker, G.B.; Rigby, J.G.

1979-07-01

In the fall of 1977, the Washington State Department of Natural Resources, Division of Geology and Earth Resources (WDGER), entered into a contract with the US Department of Energy, administered by Rockwell Hanford Operations (Rockwell) in Richland, Washington, as a principal contributor to a geologic study of feasibility of storing radioactive waste within Columbia River basalt. WDGER's responsibility was the production of this bibliography and a reconnaissance geologic map of the sediments overlying the Columbia River Basalt Group in the State of Washington. This bibliography is a compilation of all known published, unpublished, and open-file references dealing with geology and geophysics of the Columbia Basin of eastern Washington. The citations were obtained primarily from the WDGER and Washington State libraries; the Geo-Ref bibliographic system was also utilized. Because the WDGER portion of the study included preparation of a reconnaissance geologic map of surficial deposits in the Columbia Basin, available references dealing with this subject have been annotated. Many abstracts in the annotated section are quotations and have been copied directly from their respective publications

Bibliography of the geology of the Columbia Basin and surrounding areas of Washington

Energy Technology Data Exchange (ETDEWEB)

Tucker, G.B.; Rigby, J.G.

1979-07-01

In the fall of 1977, the Washington State Department of Natural Resources, Division of Geology and Earth Resources (WDGER), entered into a contract with the US Department of Energy, administered by Rockwell Hanford Operations (Rockwell) in Richland, Washington, as a principal contributor to a geologic study of feasibility of storing radioactive waste within Columbia River basalt. WDGER's responsibility was the production of this bibliography and a reconnaissance geologic map of the sediments overlying the Columbia River Basalt Group in the State of Washington. This bibliography is a compilation of all known published, unpublished, and open-file references dealing with geology and geophysics of the Columbia Basin of eastern Washington. The citations were obtained primarily from the WDGER and Washington State libraries; the Geo-Ref bibliographic system was also utilized. Because the WDGER portion of the study included preparation of a reconnaissance geologic map of surficial deposits in the Columbia Basin, available references dealing with this subject have been annotated. Many abstracts in the annotated section are quotations and have been copied directly from their respective publications.

Death with dignity in Washington patients with amyotrophic lateral sclerosis.

Science.gov (United States)

Wang, Leo H; Elliott, Michael A; Jung Henson, Lily; Gerena-Maldonado, Elba; Strom, Susan; Downing, Sharon; Vetrovs, Jennifer; Kayihan, Paige; Paul, Piper; Kennedy, Kate; Benditt, Joshua O; Weiss, Michael D

2016-11-15

To describe the amyotrophic lateral sclerosis (ALS) patients who sought medication under the Washington State Death with Dignity (DWD) Act since its inception in 2009. Chart review at 3 tertiary medical centers in the Seattle/Puget Sound region and comparison to publicly available data of ALS and all-cause DWD cohorts from Washington and Oregon. In Washington State, 39 patients with ALS requested DWD from the University of Washington, Virginia Mason, and Swedish Medical Centers beginning in 2009. The median age at death was 65 years (range 46-86). Seventy-seven percent of the patients used the prescriptions. All of the patients who used the medications passed away without complications. The major reasons for patients to request DWD as reported by participating physicians were loss of autonomy and dignity and decrease in enjoyable activities. Inadequate pain control, financial cost, and loss of bodily control were less commonly indicated. These findings were similar to those of the 92 patients who sought DWD in Oregon. In Washington and Oregon, the percentage of patients with ALS seeking DWD is higher compared to the cancer DWD cohort. Furthermore, compared to the all-cause DWD cohort, patients with ALS are more likely to be non-Hispanic white, married, educated, enrolled in hospice, and to have died at home. Although a small number, ALS represents the disease with the highest proportion of patients seeking to participate in DWD. Patients with ALS who choose DWD are well-educated and have access to palliative or life-prolonging care. The use of the medications appears to be able to achieve the patients' goals without complications. © 2016 American Academy of Neurology.

78 FR 59964 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2013-09-30

....S.C. 3003, of the completion of an inventory of human remains under the control of the Burke Museum....R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of... Washington (Burke Museum), and Central Washington University have completed an inventory of human remains, in...

Fuel management at Washington State Ferries

International Nuclear Information System (INIS)

Brodeur, P.; Olds, J.

2008-01-01

This presentation discussed Washington State Ferry (WSF) operations and provided details of a biodiesel research and demonstration project. Washington has the largest ferry system in the United States, with a total of 28 vessels that operate on 10 routes through 20 terminals. Routes vary by transit times, navigational challenges, and the proximity to population centres. WSF fuel and emissions management initiatives include exhaust emission studies, clean fuel initiatives, machinery upgrades, fuel conservation initiatives, and biodiesel testing. The organization is also using waste heat recovery and a positive restraint system. The WSF biodiesel pilot program was conducted using soy-derived fuels with a purifier disk stack. The program is in agreement with recent legislation requiring that 2 per cent of annual diesel fuel sales are from biodiesel fuels, and state legislation requiring that state agencies use a minimum of 20 per cent biodiesel blends in diesel-powered vessels and vehicles. Details of project partnerships were included. tabs., figs

Low-temperature geothermal resources of Washington

Energy Technology Data Exchange (ETDEWEB)

Schuster, J.E. [Washington State Dept. of Natural Resources, Olympia, WA (United States). Div. of Geology and Earth Resources; Bloomquist, R.G. [Washington State Energy Office, Olympia, WA (United States)

1994-06-01

This report presents information on the location, physical characteristics, and water chemistry of low-temperature geothermal resources in Washington. The database includes 941 thermal (>20C or 68F) wells, 34 thermal springs, lakes, and fumaroles, and 238 chemical analyses. Most thermal springs occur in the Cascade Range, and many are associated with stratovolcanoes. In contrast, 97 percent of thermal wells are located in the Columbia Basin of southeastern Washington. Some 83.5 percent are located in Adams, Benton, Franklin, Grant, Walla Walla, and Yakima Counties. Yakima County, with 259 thermal wells, has the most. Thermal wells do not seem to owe their origin to local sources of heat, such as cooling magma in the Earth`s upper crust, but to moderate to deep circulation of ground water in extensive aquifers of the Columbia River Basalt Group and interflow sedimentary deposits, under the influence of a moderately elevated (41C/km) average geothermal gradient.

Análise das potencialidades e barreiras referentes ao desenvolvimento e à implementação do biodiesel a partir do óleo de palma na Colômbia

OpenAIRE

Ariel Augusto Amaya Avila

2009-01-01

A Colômbia, a partir de 2003, prevendo um déficit na sua produção de combustíveis e tentando reativar a economia rural, iniciou seu programa nacional de biocombustíveis apoiado em políticas, regulamentos e incentivos econômicos para o novo setor energético. É assim que esta dissertação tem como objetivo descrever e analisar o processo de implementação do programa de biodiesel na Colômbia a partir de óleo de palma, determinando a capacidade produtiva do país e discutindo as barreiras existente...

Images and Memories: photography as document and research source for the historical recovery of Colônia Esperança (1935-1963 Imagens e Memórias: a fotografia como documento e fonte de pesquisa para a recuperação histórica da Colônia Esperança (1935-1963

Directory of Open Access Journals (Sweden)

Larissa Ayumi Sato

2011-08-01

Full Text Available The use of photography as research source and historical document has been set as important instrument in the process of historical recovery of recent settlement places as Colônia Esperança, rural community located in the city of Arapongas, on the border of Apucarana. This study considers the rescue of this community’s ancient images and the search of details that can contextualize this pictures become important tools for the recovery of scenarios that nowadays are modified by the passage of the time. In the case of Colônia Esperança, that appeared as a redoubt of catholic Japanese people searching for more fertile lands and better life conditions, around a common ideology (keep their religious practices and the customs of their origin country, this kind of analysis become particularly relevant due to the history of this location is not properly systematized. The colony was founded in 1935, even before the city of Arapongas, that was started in 1937, product of colonization works made by Companhia de Terras Norte do Paraná. This sense, this research uses as methodological procedures the reading, the iconographic analysis and the iconological interpretation of photography’s elements, bibliographic and documentary research, in addition to the oral history – as technique – to salvage colonization and everyday life aspects in the initial decades (30’s to 60’s in the location. In the end of this project, it’s expected the obtained systematization about the history of Colônia Esperança, enriched with rare pictures that were in hand of the pioneer families, unpublished testimonials and documents, fundamental for this process, can contribute to evidence the role of the photography as historical document and research source. A utilização de fotografias como fonte de pesquisa e documento histórico tem se configurado como importante instrumento no processo de reconstituição da história de lugares de coloniza-ção recente, como a

Southwestern Washington 1/3 arc-second DEM

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The 1/3-second Southwest Washington Elevation Grid provides bathymetric data in ASCII raster format of 1/3-second resolution in geographic coordinates. This grid is...

A 2015 comparison of operational performance : Washington state ferries to ferry operators worldwide.

Science.gov (United States)

2015-03-01

This report provides an update to the 2010 report A Comparison of Operational Performance: : Washington State Ferries to Ferry Operators Worldwide, observing changes in Washington State : Ferries, 23 other ferry systems, and the ferry industry ...

Willapa Bay, Washington Benthic Habitats 1995 Biotic

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — In June 1995, the Columbia River Estuary Study Taskforce (CREST) acquired 295 true color aerial photographs (1:12,000) of Willapa Bay, Washington, from the State of...

Willapa Bay, Washington Benthic Habitats 1995 Geoform

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — In June 1995, the Columbia River Estuary Study Taskforce (CREST) acquired 295 true color aerial photographs (1:12,000) of Willapa Bay, Washington, from the State of...

Willapa Bay, Washington Benthic Habitats 1995 Substrate

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — In June 1995, the Columbia River Estuary Study Taskforce (CREST) acquired 295 true color aerial photographs (1:12,000) of Willapa Bay, Washington, from the State of...

LiDAR (Terrain), THURSTON COUNTY, WASHINGTON, USA

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — Fugro EarthData Company furnished the collection, processing, and development of LiDAR for 825 square miles in Washington (805 square miles of Thurston County and 20...

Expressão citofotométrica do fator de proliferação celular ki-67 no bócio colóide e no carcinoma papilífero da tireóide

Directory of Open Access Journals (Sweden)

Gleim Dias de Souza

Full Text Available OBJETIVO: Comparar a expressão citofotométrica quantitativa do fator de proliferação celular Ki-67 no bócio colóide com o do carcinoma papilífero da tireóide. MÉTODOS: Foram estudadas a expressão da proteína Ki-67, em 12 casos de bócio colóide da tireóide e 20 casos de carcinoma papilífero da tireóide. Os núcleos celulares imunomarcados foram quantificados através do software SAMBA 4000 ® e do software IMMUNO®, analisando o índice de marcagem e densidade óptica. Foi estimado o coeficiente de correlação de Spearmane e o teste não-paramétrico de Mann-Whitney. RESULTADOS: Foi rejeitada a hipótese nula para o índice de marcagem. confirmando que existe diferença significativa entre o bócio colóide e o carcinoma papilífero da tireóide, quanto ao índice de marcagem do Ki-67, que são maiores nos carcinomas papilíferos da tireóide. Não foi encontrada diferença quanto à densidade óptica. Quanto ao bócio colóide, o coeficiente de correlação estimado entre o índice de marcagem e a densidade óptica do Ki-67 foi igual a 0,78. No bócio colóide, houve associação positiva e significativa entre o índice de marcagem e a densidade ótica do Ki-67. Para o carcinoma papilífero da tireóide o coeficiente de correlação estimado entre o índice de marcagem e a densidade ótica do Ki-67 foi igual a 0,18. Não houve no carcinoma papilífero de tireóide, associação entre o índice de marcagem e a densidade ótica do Ki-67. CONCLUSÃO: A expressão citofotométrica do Ki67 no bócio colóide teve índice médio de marcação de 13,92% e densidade óptica média de 36,43; a expressão citofotométrica do Ki-67 no carcinoma papilífero teve índice médio de marcação de 38,29% e densidade óptica média de 48,07%; há maior proliferação celular no carcinoma papilífero em comparação com o bócio colóide na expressão do Ki-67.

78 FR 17229 - Notice of Public Meeting, Eastern Washington Resource Advisory Council

Science.gov (United States)

2013-03-20

... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLORW00000 L102000000.ML0000 13XL1109AF.HAG13-0139] Notice of Public Meeting, Eastern Washington Resource Advisory Council AGENCY: Bureau of... the Eastern Washington Resource Advisory Council. The meeting on March 21, 2013, has been cancelled...

Improving Immunization Coverage in a Rural School District in Pierce County, Washington

Science.gov (United States)

Peterson, Robin M.; Cook, Carolyn; Yerxa, Mary E.; Marshall, James H.; Pulos, Elizabeth; Rollosson, Matthew P.

2012-01-01

Washington State has some of the highest percentages of school immunization exemptions in the country. We compared school immunization records in a rural school district in Pierce County, Washington, to immunization records in the state immunization information system (IIS) and parent-held records. Correcting school immunization records resulted…

76 FR 58031 - Notice of Intent To Repatriate Cultural Items: Thomas Burke Memorial Washington State Museum...

Science.gov (United States)

2011-09-19

... DEPARTMENT OF THE INTERIOR National Park Service [2253-665] Notice of Intent To Repatriate Cultural Items: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum...

El Consenso de Washington: aciertos, yerros y omisiones

Directory of Open Access Journals (Sweden)

Juan Carlos Moreno-Brid

2004-12-01

Full Text Available This paper analyses the economic and social development of Latin America after nearly two decades of macroeconomic policies and reforms in line with the “Washington Consensus”. It shows that these policies did lower inflation and induced an export boom, but failed to boost domestic investment and to remove the balance of payments binding constraint on the region’s long–term path of economic expansion. Four alternative explanations of such poor performance of the Washington Consensus are compared. It is argued, in particular, that, contrary to mainstream opinion, in Latin America there is no clear association between the depth of macroeconomic reforms and economic growth performance.

Doctors of Osteopathy Licensed in Washington.

Science.gov (United States)

Senters, Jo

Based on information gathered by the Health Manpower Project through a survey cosponsored with the Washington Osteopathic Medical Association, this report begins with a statement of philosophy of osteopathic medicine and proceeds to comment on where such professional education is available. Remarks on the type of educational background of the…

Changing Housing Patterns in Metropolitan Washington

Science.gov (United States)

Grier, George; Grier, Eunice

1975-01-01

This testimony, before a public hearing of the New York City Commission on Human Rights in May 1974, summarizes extensive studies of changing minority residential patterns in metropolitan Washington and less extensive studies of other groups; the prospects for future desegregation and for using the growing economic potential of minority families…

Timber resource statistics for western Washington.

Science.gov (United States)

Coffin D. MacLean; Patricia M. Bassett; Glenn. Yeary

1992-01-01

This report summarizes a 1988-90 timber resource inventory of 19 counties in western Washington: Clallam, Clark, Cowlitz, Grays Harbor, Island, Jefferson, King, Kitsap, Lewis, Mason, Pacific, Pierce, San Juan, Skagit, Skamania, Snohomish, Thurston, Wahkiakum, and Whatcom. Detailed tables of forest area, timber volume, growth, mortality, and harvest are presented.

78 FR 53235 - 50th Anniversary of the March on Washington for Jobs and Freedom

Science.gov (United States)

2013-08-28

... Anniversary of the March on Washington for Jobs and Freedom By the President of the United States of America A... waters of the reflecting pool, to the proud base of the Washington Monument. They were men and women..., and justice for all. The March on Washington capped off a summer of discontent, a time when the...

A Grape Production Guide for Vocational Agriculture Instructors in Washington. Final Report.

Science.gov (United States)

Padelford, Stewart L.; Cvancara, Joseph G., Ed.

This curriculum guide is intended to provide vocational agriculture instructors with an up-to-date resource dealing with grape production in Washington. Addressed in the individual units of the guide are the following topics: the history of grape production; grape types important to Washington; site selection for a vineyard; establishment and…

Medicinal Cannabis: A Survey Among Health Care Providers in Washington State

Science.gov (United States)

Carlini, Beatriz H; Garrett, Sharon B; Carter, Gregory T

2017-02-01

Washington State allows marijuana use for medical (since 1998) and recreational (since 2012) purposes. The benefits of medicinal cannabis (MC) can be maximized if clinicians educate patients about dosing, routes of administration, side effects, and plant composition. However, little is known about clinicians' knowledge and practices in Washington State. An anonymous online survey assessed providers' MC knowledge, beliefs, clinical practices, and training needs. The survey was disseminated through health care providers' professional organizations in Washington State. Descriptive analysis compared providers who had and had not authorized MC for patients. Survey results informed the approach and content of an online training on best clinical practices of MC. Four hundred ninety-four health care providers responded to the survey. Approximately two-third were women, aged 30 to 60 years, and working in family or internal medicine. More than half of the respondents were legally allowed to write MC authorizations per Washington State law, and 27% of those had issued written MC authorizations. Overall, respondents reported low knowledge and comfort level related to recommending MC. Respondents rated MC knowledge as important and supported inclusion of MC training in medical/health provider curriculum. Most Washington State providers have not received education on scientific basis of MC or training on best clinical practices of MC. Clinicians who had issued MC authorizations were more likely to have received MC training than those who had not issued MC authorization. The potential of MCs to benefit some patients is hindered by the lack of comfort of clinicians to recommend it. Training opportunities are badly needed to address these issues.

Basic Fibroblast Growth Factor Fused with Tandem Collagen-Binding Domains from Clostridium histolyticum Collagenase ColG Increases Bone Formation

Directory of Open Access Journals (Sweden)

Hiroyuki Sekiguchi

2018-01-01

Full Text Available Basic fibroblast growth factor 2 (bFGF accelerates bone formation during fracture healing. Because the efficacy of bFGF decreases rapidly following its diffusion from fracture sites, however, repeated dosing is required to ensure a sustained therapeutic effect. We previously developed a fusion protein comprising bFGF, a polycystic kidney disease domain (PKD; s2b, and collagen-binding domain (CBD; s3 sourced from the Clostridium histolyticum class II collagenase, ColH, and reported that the combination of this fusion protein with a collagen-like peptide, poly(Pro-Hyp-Gly10, induced mesenchymal cell proliferation and callus formation at fracture sites. In addition, C. histolyticum produces class I collagenase (ColG with tandem CBDs (s3a and s3b at the C-terminus. We therefore hypothesized that a bFGF fusion protein containing ColG-derived tandem CBDs (s3a and s3b would show enhanced collagen-binding activity, leading to improved bone formation. Here, we examined the binding affinity of four collagen anchors derived from the two clostridial collagenases to H-Gly-Pro-Arg-Gly-(Pro-Hyp-Gly12-NH2, a collagenous peptide, by surface plasmon resonance and found that tandem CBDs (s3a-s3b have the highest affinity for the collagenous peptide. We also constructed four fusion proteins consisting of bFGF and s3 (bFGF-s3, s2b-s3b (bFGF-s2b-s3, s3b (bFGF-s3b, and s3a-s3b (bFGF-s3a-s3b and compared their biological activities to those of a previous fusion construct (bFGF-s2b-s3 using a cell proliferation assay in vitro and a mouse femoral fracture model in vivo. Among these CB-bFGFs, bFGF-s3a-s3b showed the highest capacity to induce mesenchymal cell proliferation and callus formation in the mice fracture model. The poly(Pro-Hyp-Gly10/bFGF-s3a-s3b construct may therefore have the potential to promote bone formation in clinical settings.

Lethal chondrodysplasia in a family of Holstein cattle is associated with a de novo splice site variant of COL2A1

DEFF Research Database (Denmark)

Agerholm, Jørgen Steen; Menzi, Fiona; McEvoy, Fintan

2016-01-01

was predicted to affect splicing as it altered the conserved splice donor sequence GT at the 5’-end of COL2A1 intron 36, which was changed to AT. All five available cases carried the mutant allele in heterozygous state and all five dams were homozygous wild type. The sire VH Cadiz Captivo was shown...

National Nuclear Waste Policy Act of 1981. Hearings before the Committee on Environment and Public Works, United States Senate, Ninety-Seventh Congress, First Session on S. 1662, October 31, 1981, Richland, Washington; November 9, 1981, Washington, DC

International Nuclear Information System (INIS)

Anon.

1982-01-01

Hearings were held on October 31, 1981 in Richland, Washington and on November 9, 1981 in Washington, DC to discuss the effort in S. 1662 to establish a national policy and an environmentally acceptable program for managing nuclear wastes from domestic commercial activities. The Richland hearing was held in recognition that Washington State will bear the major impact of the legislation. Witnesses at the Washington, DC hearing included officials from states that are potential sites for radioactive waste storage and disposal facilities. The hearing record includes the testimony of 16 witnesses in Richland and seven in Washington, DC, followed by a reprint of S. 1662 and additional material submitted for the record

Embryo genome profiling by single-cell sequencing for successful preimplantation genetic diagnosis in a family harboring COL4A1 c.1537G>A; p.G513S mutation

Directory of Open Access Journals (Sweden)

Nayana H Patel

2016-01-01

Full Text Available CONTEXT: Genetic profiling of embryos (also known as preimplantation genetic diagnosis before implantation has dramatically enhanced the success quotient of in vitro fertilization (IVF in recent times. The technology helps in avoiding selective pregnancy termination since the baby is likely to be free of the disease under consideration. AIM: Screening of embryos free from c.1537G>A; p.G513S mutation within the COL4A1 gene for which the father was known in before be in heterozygous condition. SUBJECTS AND METHODS: Processing of trophectoderm biopsies was done from twelve embryos for c.1537G>A; p.G513S mutation within the COL4A1 gene. DNA extracted from isolated cells were subjected to whole genome amplification using an isothermal amplification and strand displacement technology. Oligonucleotide primers bracketing the mutation were synthesized and used to amplify 162 base pairs (bp polymerase chain reaction amplicons originating from each embryo which were subsequently sequenced to detect the presence or absence of the single base polymorphism. RESULTS: Three out of 12 embryos interrogated in this study were found to be normal while 9 were found to harbor the mutation in heterozygous condition. Implantation of one of the normal embryos following by chorionic villus sampling at 11 th week of pregnancy indicated that the baby was free from c.1537G>A; p.G513S mutation within the COL4A1 gene. CONCLUSIONS: Single-cell sequencing is a helpful tool for preimplantation embryo profiling. This is the first report from India describing the birth of a normal child through IVF procedure where a potential pathogenic COL4A1 allele was avoided using this technology.

Washington Irving and the American Indian.

Science.gov (United States)

Littlefield, Daniel F., Jr.

1979-01-01

Some modern scholars feel that Washington Irving vacillated between romanticism and realism in his literary treatment of the American Indian. However, a study of all his works dealing with Indians, placed in context with his non-Indian works, reveals that his attitude towards Indians was intelligent and enlightened for his time. (CM)

Upregulated expression of integrin α1 in mesangial cells and integrin α3 and vimentin in podocytes of Col4a3-null (Alport mice.

Directory of Open Access Journals (Sweden)

Brooke M Steenhard

Full Text Available Alport disease in humans, which usually results in proteinuria and kidney failure, is caused by mutations to the COL4A3, COL4A4, or COL4A5 genes, and absence of collagen α3α4α5(IV networks found in mature kidney glomerular basement membrane (GBM. The Alport mouse harbors a deletion of the Col4a3 gene, which also results in the lack of GBM collagen α3α4α5(IV. This animal model shares many features with human Alport patients, including the retention of collagen α1α2α1(IV in GBMs, effacement of podocyte foot processes, gradual loss of glomerular barrier properties, and progression to renal failure. To learn more about the pathogenesis of Alport disease, we undertook a discovery proteomics approach to identify proteins that were differentially expressed in glomeruli purified from Alport and wild-type mouse kidneys. Pairs of cy3- and cy5-labeled extracts from 5-week old Alport and wild-type glomeruli, respectively, underwent 2-dimensional difference gel electrophoresis. Differentially expressed proteins were digested with trypsin and prepared for mass spectrometry, peptide ion mapping/fingerprinting, and protein identification through database searching. The intermediate filament protein, vimentin, was upregulated ∼2.5 fold in Alport glomeruli compared to wild-type. Upregulation was confirmed by quantitative real time RT-PCR of isolated Alport glomeruli (5.4 fold over wild-type, and quantitative confocal immunofluorescence microscopy localized over-expressed vimentin specifically to Alport podocytes. We next hypothesized that increases in vimentin abundance might affect the basement membrane protein receptors, integrins, and screened Alport and wild-type glomeruli for expression of integrins likely to be the main receptors for GBM type IV collagen and laminin. Quantitative immunofluorescence showed an increase in integrin α1 expression in Alport mesangial cells and an increase in integrin α3 in Alport podocytes. We conclude that

Influence of tra genes of IncP and F plasmids on the mobilization of small Kanamycin resistance ColE1-Like plasmids in bacterial biofilms

Science.gov (United States)

Background: Horizontal gene transfer is a mechanism for movement of antibiotic resistance genes among bacteria. Some small kanamycin resistance (KanR) ColE1-like plasmids isolated from different serotypes of Salmonella enterica were shown to carry mobilization genes; although not self-transmissibl...

Report : public transportation in Washington State, 1984

Science.gov (United States)

1984-10-01

This report is an update of the Public Transportation in Washington State publication, dated December 1981. In order to reflect the changes that have occurred since that time, this report contains the most current data obtainable. Chapter One of this...

Scientific Framework for Stormwater Monitoring by the Washington State Department of Transportation

Science.gov (United States)

Sheibley, R.W.; Kelly, V.J.; Wagner, R.J.

2009-01-01

The Washington State Department of Transportation municipal stormwater monitoring program, in operation for about 8 years, never has received an external, objective assessment. In addition, the Washington State Department of Transportation would like to identify the standard operating procedures and quality assurance protocols that must be adopted so that their monitoring program will meet the requirements of the new National Pollutant Discharge Elimination System municipal stormwater permit. As a result, in March 2009, the Washington State Department of Transportation asked the U.S. Geological Survey to assess their pre-2009 municipal stormwater monitoring program. This report presents guidelines developed for the Washington State Department of Transportation to meet new permit requirements and regional/national stormwater monitoring standards to ensure that adequate processes and procedures are identified to collect high-quality, scientifically defensible municipal stormwater monitoring data. These include: (1) development of coherent vision and cooperation among all elements of the program; (2) a comprehensive approach for site selection; (3) an effective quality assurance program for field, laboratory, and data management; and (4) an adequate database and data management system.

Westport, Washington Tsunami Forecast Grids for MOST Model

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The Westport, Washington Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model....

The Path to Advanced Practice Licensure for Clinical Nurse Specialists in Washington State.

Science.gov (United States)

Schoonover, Heather

The aim of this study was to provide a review of the history and process to obtaining advanced practice licensure for clinical nurse specialists in Washington State. Before 2016, Washington State licensed certified nurse practitioners, certified nurse midwives, and certified nurse anesthetists under the designation of an advanced registered nurse practitioner; however, the state did not recognize clinical nurse specialists as advanced practice nurses. The work to drive the rule change began in 2007. The Washington Affiliate of the National Association of Clinical Nurse Specialists used the Power Elite Theory to guide advocacy activities, building coalitions and support for the desired rule changes. On January 8, 2016, the Washington State Nursing Care Quality Assurance Commission voted to amend the state's advanced practice rules, including clinical nurse specialists in the designation of an advanced practice nurse. Since the rule revision, clinical nurse specialists in Washington State have been granted advanced registered nurse practitioner licenses. Driving changes in state regulatory rules requires diligent advocacy, partnership, and a deep understanding of the state's rule-making processes. To be successful in changing rules, clinical nurse specialists must build strong partnerships with key influencers and understand the steps in practice required to make the desired changes.

78 FR 2429 - Notice of Inventory Completion: The Museum of Anthropology at Washington State University...

Science.gov (United States)

2013-01-11

... construction. Archaeologists working for the Washington Archaeological Research Center at Washington State... Reservation. Historical, ethnographic, linguistic, and archaeological information links the site to the...

Detection of mutations in the COL4A5 gene by SSCP in X-linked Alport syndrome

DEFF Research Database (Denmark)

Hertz, Jens Michael; Juncker, I; Persson, U

2001-01-01

, three in-frame deletions, four nonsense mutations, and six splice site mutations. Twenty-two of the mutations have not previously been reported. Furthermore, we found one non-pathogenic amino acid substitution, one rare variant in a non-coding region, and one polymorphism with a heterozygosity of 28...... of type IV-collagen. We performed mutation analysis of the COL4A5 gene by PCR-SSCP analysis of each of the 51 exons with flanking intronic sequences in 81 patients suspected of X-linked Alport syndrome including 29 clear X-linked cases, 37 cases from families with a pedigree compatible with X...

Evaluating ballot initiative support for legalised marijuana: The case of Washington.

Science.gov (United States)

Collingwood, Loren; O'Brien, Ben Gonzalez; Dreier, Sarah

2018-03-11

In 2012, Washington and Colorado became the first U.S. states to legalise recreational marijuana. By 2016, eight states and the District of Columbia had legalised recreational marijuana, with more expected to consider it in 2018. Despite this trend, little academic research explains what drives ballot-initiative vote choice on marijuana legalisation. This paper uses a pre-election random sample voter survey to examine the individual characteristics that correlated with Washington voters' support for legal recreational marijuana. We find that voting on marijuana ballot initiatives largely reflects public opinion about marijuana and is particularly shaped voters' political ideology, party affiliation, religious affiliation and practice, and education. Notably, we find that those reporting experiences (i.e., someone they know) with the criminal justice system are more supportive of legalisation than those who do not. We conclude that marijuana legalisation voting behavior generally aligns with public opinion on the issue. However, one key aspect of Washington's legalisation campaign-the criminal injustices of marijuana illegality-helped shape Washington state voting behavior. Further research is needed to examine if, when, and in what contexts criminal justice campaign themes are likely to strengthen or undermine future states' marijuana legalisation efforts. Copyright © 2018 Elsevier B.V. All rights reserved.

Crash Fatality Rates After Recreational Marijuana Legalization in Washington and Colorado.

Science.gov (United States)

Aydelotte, Jayson D; Brown, Lawrence H; Luftman, Kevin M; Mardock, Alexandra L; Teixeira, Pedro G R; Coopwood, Ben; Brown, Carlos V R

2017-08-01

To evaluate motor vehicle crash fatality rates in the first 2 states with recreational marijuana legalization and compare them with motor vehicle crash fatality rates in similar states without recreational marijuana legalization. We used the US Fatality Analysis Reporting System to determine the annual numbers of motor vehicle crash fatalities between 2009 and 2015 in Washington, Colorado, and 8 control states. We compared year-over-year changes in motor vehicle crash fatality rates (per billion vehicle miles traveled) before and after recreational marijuana legalization with a difference-in-differences approach that controlled for underlying time trends and state-specific population, economic, and traffic characteristics. Pre-recreational marijuana legalization annual changes in motor vehicle crash fatality rates for Washington and Colorado were similar to those for the control states. Post-recreational marijuana legalization changes in motor vehicle crash fatality rates for Washington and Colorado also did not significantly differ from those for the control states (adjusted difference-in-differences coefficient = +0.2 fatalities/billion vehicle miles traveled; 95% confidence interval = -0.4, +0.9). Three years after recreational marijuana legalization, changes in motor vehicle crash fatality rates for Washington and Colorado were not statistically different from those in similar states without recreational marijuana legalization. Future studies over a longer time remain warranted.

Natural phenomena hazards, Hanford Site, Washington

International Nuclear Information System (INIS)

Conrads, T.J.

1998-01-01

This document presents the natural phenomena hazard loads for use in implementing DOE Order 5480.28, Natural Phenomena Hazards Mitigation, and supports development of double-shell tank systems specifications at the Hanford Site in south-central Washington State. The natural phenomena covered are seismic, flood, wind, volcanic ash, lightning, snow, temperature, solar radiation, suspended sediment, and relative humidity

DESARROLLO DE COMPETENCIAS EN LOS ESTUDIANTES DEL LABORATORIO DE MATERIALES DEL PROGRAMA DE INGENIERÍA MECÁNICA EN EL NÚCLEO LUZ-COL.

Directory of Open Access Journals (Sweden)

Raysa Balza

2012-11-01

Full Text Available Esta investigación tiene como objetivo analizar las estrategias que contribuyen al desarrollo de competencias en los estudiantes del laboratorio de Materiales en el Programa de Ingeniería Mecánica Núcleo LUZ-COL. La investigación se catalogó como descriptiva, con un diseño de campo, no experimental, transeccional (Hernández y col., 2003. La población estuvo integrada or seis (6 docentes y 365 estudiantes. Seleccionando una muestra de 191 estudiantes, los otros elementos de la población fueron considerados en su totalidad. Se utilizaron como instrumentos una guía de observación y la entrevista estructurada. Evidenciando que el laboratorio presenta debilidades tanto en los aspectos infraestructurales como académicos; las estrategias utilizadas contribuyen medianamente a la formación de competencias en los estudiantes a través de un aprendizaje cooperativo. Asimismo, los equipos utilizados no cumplen con los aspectos tecnológicos y didácticos para el desarrollo de las prácticas.

76 FR 14045 - Notice of Intent To Repatriate a Cultural Item: Museum of Anthropology at Washington State...

Science.gov (United States)

2011-03-15

...: Museum of Anthropology at Washington State University, Pullman, WA AGENCY: National Park Service... Museum of Anthropology at Washington State University, Pullman, WA, that meets the definition of..., a copper pendant was given to the Museum of Anthropology at Washington State University for intended...

Washington Windplant No. 1: Botanical resources field survey. Appendix B to Washington Windplant No. 1 EIS

International Nuclear Information System (INIS)

1994-12-01

KENETECH Windpower, Inc., has applied to Klickitat County for a conditional use permit to construct and operate a 115 Megawatt windfarm on an approximately 5110 hectare (12,630 acre) site in the Columbia Hills near Goldendale, Washington. A transmission services agreement between the Bonneville Power Administration (BPA) and utilities purchasing the Project's output will also be required. Klickitat County and BPA initiated the preparation of a joint SEPA/NEPA Environmental Impact Statement, under the authority of the Washington State Environmental Policy Act and the National Environmental Policy Act. The EIS addresses, among other issues, the Project's potential impact on rare, threatened or endangered, and sensitive plant species and communities as well as plant species of potential cultural importance. A field survey along potential impact corridors (turbine strings, roadways, and the transmission line alignment) was conducted between April and June, 1994 in order to identify rare plant species, high-quality native plant communities, and plant species of potential cultural importance present in these corridors. In addition, habitat maps of the entire 5110-ha project area were field verified. This report contains the results of that survey and an assessment of the potential project impacts

History of Cardiothoracic Surgery at Washington University in Saint Louis.

Science.gov (United States)

Moon, Marc R

2016-01-01

The Division of Cardiothoracic Surgery at Washington University evolved a century ago to address what many considered to be the last surgical frontier, diseases of the chest. In addition, as one of the first training programs in thoracic surgery, Washington University has been responsible for educating more thoracic surgeons than nearly any other program in the world. Beginning with Evarts A. Graham and continuing through to Ralph J. Damiano Jr., the leaders of the division have had a profound impact on the field of cardiothoracic surgery. Copyright © 2016 Elsevier Inc. All rights reserved.

Somatic mosaicism for the COL7A1 mutation p.Gly2034Arg in the unaffected mother of a patient with dystrophic epidermolysis bullosa pruriginosa

NARCIS (Netherlands)

van den Akker, P. C.; Pasmooij, A. M. G.; Meijer, R.; Scheffer, H.; Jonkman, M. F.

Dystrophic epidermolysis bullosa (DEB) is a heritable blistering disorder caused by mutations in the type VII collagen gene, COL7A1. Although revertant mosaicism is well known in DEB, 'forward' somatic mosaicism, in which a pathogenic mutation arises on a wild-type (WT) background, extending beyond

Somatic mosaicism for the COL7A1 mutation p.Gly2034Arg in the unaffected mother of a patient with dystrophic epidermolysis bullosa pruriginosa

NARCIS (Netherlands)

Akker, P.C. van den; Pasmooij, A.M.; Meijer, R.; Scheffer, H.; Jonkman, M.F.

2015-01-01

Dystrophic epidermolysis bullosa (DEB) is a heritable blistering disorder caused by mutations in the type VII collagen gene, COL7A1. Although revertant mosaicism is well known in DEB, 'forward' somatic mosaicism, in which a pathogenic mutation arises on a wild-type (WT) background, extending beyond

Metais pesados, agrotóxicos e combustíveis: efeito na população de colêmbolos no solo Heavy metal, pesticides and fuels: effect in the population of collembola in the soil

Directory of Open Access Journals (Sweden)

Zaida Inês Antoniolli

2013-06-01

Full Text Available O objetivo deste trabalho foi avaliar o desenvolvimento de colêmbolos no solo com diferentes níveis de metais pesados, combustíveis e agrotóxicos em condição de laboratório. Os tratamentos foram: Cádmio (1, 10 e 100mg kg-1 de solo; Cobre (50, 500 e 5000mg kg-1 de solo; Zinco (100, 1000 e 10000mg kg-1 de solo; fungicida epoxiconazol + piraclostrobina (1 e 2L ha-1; fungicida epoxiconazol (0,75 e 1,5L ha-1; herbicida glifosato (2 e 4L ha-1; óleo lubrificante queimado (5, 50 e 100mL kg-1 solo e óleo diesel (5, 50 e 100mL kg-1 solo e controle. Na dose de 1mg kg-1 de Cd no solo, houve grande aumento na população de colêmbolos e o pH do solo diminuiu à medida que aumentaram as doses de Cd, Zn e Cu no solo. Na presença de combustíveis, os colêmbolos apresentaram incapacidade de reprodução, independente da dose aplicada no solo. O número de colêmbolos apresentou incremento com o aumento da dosagem de glifosato e epoxiconazol. Os metais pesados Cu e Zn têm ação negativa sobre a população de colêmbolos e somente o Cd (1mg kg-1 solo proporciona aumento na população de colêmbolos no solo. A presença de óleo lubrificante queimado e óleo diesel no solo inibem o desenvolvimento dos colêmbolos no solo. Os agrotóxicos glifosato, epoxiconazol e epoxiconazol + piraclostrobina não influenciam negativamente sobre a população de colêmbolos no solo.The aim of this research was to evaluate the collembola development in soils with different levels of heavy metals, fuels and pesticides, in laboratory conditions. Treatments were: Cadmium - 1, 10 and 100mg kg-1 soil; Copper - 50, 500 and 5000mg kg-1 soil; Zinc - 100, 1000 and 10000 mg kg-1 soil; fungicide epoxiconazol + piraclostrobina - 1 and 2L ha-1; fungicide epoxiconazol - 0,75 and 1,5L ha-1; herbicide glyphosate - 2 and 4L ha-1; burned lubricating oil - 5, 50 and 100mL kg-1 soil and diesel oil - 5, 50 and 100mL kg-1 soil and control. In the 1mg kg-1 dose of Cd there was a great

Basic Education for Adults: Pathways to College and Careers for Washington's Emerging Workforce. Washington's Community and Technical Colleges

Science.gov (United States)

Washington State Board for Community and Technical Colleges, 2015

2015-01-01

This brief describes the Basic Education for Adults (BEdA) programs that bridge the gap between school and work, thereby creating pathways to college and careers for Washington's emerging workforce. BEdA programs teach foundational skills--reading, writing, math, technology and English language--so adults can move through college and into…

76 FR 28066 - Notice of Intent To Repatriate a Cultural Item: Museum of Anthropology at Washington State...

Science.gov (United States)

2011-05-13

... Cultural Item: Museum of Anthropology at Washington State University, Pullman, WA AGENCY: National Park... possession of the Museum of Anthropology at Washington State University, Pullman, WA, that meets the... notice. One lot of stone, bone, and glass beads was given to the Museum of Anthropology at Washington...

Integrated Basic Education and Skills Training (I-BEST). Washington's Community and Technical Colleges

Science.gov (United States)

Washington State Board for Community and Technical Colleges, 2014

2014-01-01

Washington's Integrated Basic Education and Skills Training Program (I-BEST) quickly teaches students literacy, work, and college-readiness skills so they can move through school and into living wage jobs faster. Pioneered by Washington's community and technical colleges, I-BEST uses a team-teaching approach to combine college-readiness classes…

Joint NEPA/SEPA draft environmental impact statement Washington Windplant No. 1

International Nuclear Information System (INIS)

1995-02-01

This is the draft Environmental Impact Statement for a proposed construction and operation of a 115 megawatt Washington Windplant No. 1 by KENETECH Windpower, Inc in Klickitat County, Washington. The Project would be constructed on private land (5,110 hectares) under easement to KENETECH Windpower, Inc., and would include approximately 345 type 33M-VS wind turbines. Alternatives to the Proposed Action include an alternative powerline route; a restricted areas alternative; a subarea development alternative; a no-action alternative

Endangered Species Case - Washington Toxics Coalition v. EPA

Science.gov (United States)

This Web page provides information on the Washington Toxics Coalition v. EPA case, related to protection of Pacific salmon and steelhead, and links to the biological opinions issued by the NMFS and EPA’s responses.

Diseño de un relleno sanitario manual para el recinto "Cristóbal Colón" - provincia de Esmeraldas

OpenAIRE

Ruiz Ortega, Jennifer Carolina; Unapanta Albán, Victoria Carolina

2015-01-01

This project, contains the design of a manual sanitary landfill for the recinto Cristóbal Colón, for which it was necessary to know the current situation, with regard to biotic, physical and socio-economic environment. First, is selected representative sample of the population, distributed among households and commercial establishments, in order to collect their waste for eight days and characterized by the quartering method. Once established the optimal way for the design of the landfi...

77 FR 61782 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

Science.gov (United States)

2012-10-11

... Washington State Parks and Recreation Commission at the address below by November 13, 2012. ADDRESSES: Alicia... affiliated with the human remains should contact Alicia Woods, Washington State Parks and Recreation...

A criação do Colégio de Pedro II e seu impacto na constituição do magistério público secundário no Brasil

Directory of Open Access Journals (Sweden)

Ana Waleska P. C. Mendonça

2013-12-01

Full Text Available Este trabalho se vincula a uma pesquisa mais ampla que tem como objeto de estudo o processo de construção de uma identidade profissional específica por parte de um segmento da categoria docente - o magistério público secundário - no Brasil, constituindo-se como um primeiro produto coletivo do grupo de investigação. A referida pesquisa parte de um recorte institucional e toma como alvo de estudo o Colégio Pedro II, que foi criado pelo governo imperial em 1837 e que, após a República, é mantido pelo governo federal, conservando, até meados do século XX, o caráter de instituição modelar para todo o ensino secundário brasileiro. O texto está dividido em duas partes. Na primeira, propõe-se a identificar o programa institucional que informou a criação do Colégio de Pedro II. Na segunda, desenvolve um estudo preliminar sobre a primeira geração de professores do Colégio, nomeados entre 1838 e 1847, buscando estabelecer uma caracterização geral: as formas de recrutamento; a distribuição pela diferentes disciplinas; as categorias docentes previstas nos estatutos de 1838; as trajetórias internas no Colégio, inclusive o tempo de docência na instituição; a formação acadêmica; a experiência docente anterior; as outras instituições educacionais/culturais em que atuavam, entre outros aspectos. As principais referências teóricas são os trabalhos de dois sociólogos franceses que se debruçaram sobre o processo de construção da identidade profissional docente, a saber: François Dubet e Claude Dubar.

78 FR 13887 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

Science.gov (United States)

2013-03-01

... Washington State Parks and Recreation Commission at the address below by April 1, 2013. ADDRESSES: Alicia... contact Alicia Woods, Washington State Parks and Recreation Commission, PO Box 42650, Olympia, WA 98504...

Washington Public Libraries Online: Collaborating in Cyberspace.

Science.gov (United States)

Wildin, Nancy

1997-01-01

Discussion of public libraries, the Internet, and the World Wide Web focuses on development of a Web site in Washington. Highlights include access to the Internet through online public access catalogs; partnerships between various types of libraries; hardware and software; HTML training; content design; graphics design; marketing; evaluation; and…

The effect of 6-week high intensity interval training on the VEGF/COL-18 ratio and some echocardiographic indices in rats with myocardial infarction

Directory of Open Access Journals (Sweden)

Sara Karbalaeifar

2016-12-01

Full Text Available Introduction: Myocardial infarction (MI is the irreversible cell death caused by ischemia in parts of myocardium. The molecular process of increased capillary density in response to activity and its appropriate intensity is not clear yet. Therefore, this research aimed to evaluate the effect of 6-week high intensity interval training on the VEGF/COL-18 ratio and echocardiographic indices in rats with MI. Methods: Twelve Wistar male rats of 10 weeks old and mean weight 250-300gr were allocated to two groups of experimental (60 minutes of interval treadmill running for four minutes with the intensity of 85-90 and two minutes of active rest at 50-60 percent of VO2max for four days a week for 6 weeks and control group (without any training. Real-time PCR was used to assess the expression of VEGF and COL-18 genes after inducing MI, and shortening fractional and ejection fraction were investigated as echocardiographic indices. Data were analyzed in SPSS18 using independent t test (α≤ 0.05. Results: The findings showed that there was no significant increase in the VEGF/COL-18 ratio in the HIIT group (1.856 mg/ml as compared with the control group (1.245 mg/ml (p=0.263. A significant increase was observed in the HIIT group for SF (77.461±7.022% and EF (41.625±6.847% as compared with the control group (64.483±3.695% and (31.320±3.460%, respectively (p=0.001. Conclusion: In general, 6 weeks of high intensity interval training can effectively increase angiogenesis factors and improve myocardial function in male Wistar rats after MI.

Xarxa interclase: un projecte col·laboratiu mitjançant tecnologies digitals

Directory of Open Access Journals (Sweden)

Françoise Olmo Cazevieille

2015-12-01

Full Text Available Focalitzem aquest treball en l'aportació de les tecnologies de la informació i la comunicació (TIC a la pràctica i el desenvolupament d'una llengua estrangera i en els avantatges que ofereixen per a la creació d'activitats basades en la col·laboració i la interacció. Sense aquestes, tant els treballs en equip amb nadius com la cerca d'informació sobre temes especialitzats en la llengua estudiada serien complexos de dur a terme. En aquest article presentem el procediment seguit en un projecte didàctic, Xarxa interclasse, entre dues assignatures basades en l'aprenentatge de llengües estrangeres per a fins específics en un mateix campus universitari.?Es pretén, d'una banda, acostar l'educació superior a les exigències de la societat actual i, d'altra banda, aconseguir una docència motivadora i una participació activa dels discents on la tecnologia s'integra com a element pedagògic.

Next-generation sequencing and a novel COL3A1 mutation associated with vascular Ehlers-Danlos syndrome with severe intestinal involvement: a case report.

Science.gov (United States)

Cortini, Francesca; Marinelli, Barbara; Seia, Manuela; De Giorgio, Barbara; Pesatori, Angela Cecilia; Montano, Nicola; Bassotti, Alessandra

2016-10-31

The vascular type of Ehlers-Danlos syndrome is an autosomal dominant connective tissue disorder caused by a mutation in the COL3A1 gene encoding pro-alpha1 chain of type III collagen. The vascular type of Ehlers-Danlos syndrome causes severe fragility of connective tissues with arterial and intestinal ruptures and complications in surgical and radiological treatments. We present a case of a 38-year-old Italian woman who was diagnosed as having the vascular type of Ehlers-Danlos syndrome. Genetic testing, conducted by Target Enrichment approach (Agilent Technologies), identified a new mutation c.1493G>A, p.G498D in exon 21 of COL3A1 gene (heterozygous state). This mutation disrupts the normal glycine-X-Y repetitions of type III procollagen by converting glycine to aspartic acid. We report a new genetic mutation associated with the vascular type of Ehlers-Danlos syndrome. We also describe clinical and genetic findings that are important to understand the genotype/phenotype correlation in patients with the vascular type of Ehlers-Danlos syndrome.

Distribuciones simuladas de valores de d13c de colágeno humano: implicancias para los estudios paleodietarios

Directory of Open Access Journals (Sweden)

Barrientos, Gustavo

2005-01-01

Full Text Available El objetivo de este trabajo es presentar los primeros resultados derivados de la aplicación de un programa de simulación de distribuciones de valores de δ13C de colágeno humano (DeltaCarb130.0, desarrollado por los autores. DeltaCarb130.0 es un programa simple que simula la composición isotópica (δ13C del colágeno de huesos humanos obtenida a través de una dieta de composición variable, constituida por tres ingestas diarias, durante un período de 1825 días (5 años. La composición de la dieta y los valores de δ13C de cada recurso dietario (vegetales C3 y C4, carne de herbívoros consumidores de C3 y C4, de mamíferos marinos, de peces y moluscos se obtienen, en cada etapa de la simulación, a través de un proceso aleatorio a partir de datos de abundancia relativa y rangos de valores de δ13C de cada tipo de recurso. Se analizarán estadísticamente las distribuciones de valores de δ13C obtenidas a partir de n corridas del programa para cada combinación dietaria, con la finalidad de obtener medidas de tendencia central y de dispersión asociadas a cada tipo de dieta y calcular la probabilidad de obtener valores "outliers", un rasgo frecuente en el estudio de casos arqueológicos.

Timber resource statistics for the Olympic Peninsula, Washington.

Science.gov (United States)

Patricia M. Bassett; Daniel D. Oswald

1961-01-01

This report summarizes a 1978-79 timber resource inventory of five counties in the Olympic Peninsula of Washington: Clallam, Grays Harbor, Jefferson, Mason, and Thurston. Detailed tables of forest area, timber volume, growth, mortality, and harvest are presented.

Recidivism of Supermax Prisoners in Washington State

Science.gov (United States)

Lovell, David; Johnson, L. Clark; Cain, Kevin C.

2007-01-01

This study of recidivism among Washington supermax prisoners used a retrospective matched control design, matching supermax prisoners one-to-one with nonsupermax prisoners on mental illness status and up to eight recidivism predictors. Supermax prisoners committed new felonies at a higher rate than nonsupermax controls, but the difference was not…

III Colóquio luso-brasileiro de educação a distância e elearning

OpenAIRE

Amante, Lúcia; Mendes, António Quintas; Barros, Daniela Melaré Vieira; Cardoso, Teresa; Spilker, Maria João

2013-01-01

O livro de atas do III Colóquio Luso-Brasileiro de Educação a Distância e Elearning, realizado em Lisboa em dezembro de 2013, reúne um vasto conjunto de comunicações apresentadas no evento e que se distribuem por 10 grandes temas a saber: EaD e Políticas educativas; Boas Práticas em Educação Online; Modelos e Processos Pedagógicos em Educação online; Web 2.0 e tecnologias emergentes; Pedagogias emergentes (PLEs, MOOCs, REAs, …); Realidade Virtual em EaD; Jogos e gamification em EaD; Mobile Le...

78 FR 78808 - Proposed Flood Elevation Determinations for Pierce County, Washington, and Incorporated Areas

Science.gov (United States)

2013-12-27

...-2013-0002; Internal Agency Docket No. FEMA-B-7748] Proposed Flood Elevation Determinations for Pierce... proposed rule concerning proposed flood elevation determinations for Pierce County, Washington, and... sources in Pierce County, Washington. On April 16, 2012, FEMA published a proposed rulemaking at 77 FR...

77 FR 48535 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

Science.gov (United States)

2012-08-14

...: Alicia Woods, Washington State Parks and Recreation Commission, P.O. Box 42650, Olympia, WA 98504-2650... it satisfies the criteria in 43 CFR 10.11(c)(1) should contact Alicia Woods, Washington State Parks...

Imagens das colônias da empresa de colonização Meyer no Rio Grande do Sul, 1906

Directory of Open Access Journals (Sweden)

Rosane Marcia Neumann

2013-04-01

Full Text Available Esse artigo, a partir da análise do prospecto fotográfico Ansichten aus Dr. Herrmann Meyers Ackerbaukolonien Neu-Württemberg und Xingu in Rio Grande do Sul (Südbrasilien, editado pelo Instituto Bibliográfico de Leipzig, em 1906, discute o uso da fotografia pela Empresa de Colonização Dr. Herrmann Meyercomo suporte para a divulgação de seu projeto de colonização, e o modo pelo qual representou as suas colônias e os seus colonos.

High School Administrative Staffing in Washington State: Principal Perspectives on Resource Needs and Utilization

Science.gov (United States)

Steach, John C.

2011-01-01

This mixed methods study explored how high school principals prioritize their work and utilize available human resources to adjust to inadequate administrative staffing. Analysis of staffing levels across the state of Washington and specifically inside two eastern Washington districts framed interview questions for central office administration…

78 FR 76031 - Sweet Cherries Grown in Designated Counties in Washington; Decreased Assessment Rate

Science.gov (United States)

2013-12-16

... FIR] Sweet Cherries Grown in Designated Counties in Washington; Decreased Assessment Rate AGENCY... subsequent fiscal periods from $0.18 to $0.15 per ton of sweet cherries handled. The Committee locally administers the marketing order for sweet cherries grown in designated counties in Washington. The Committee's...

Early Restoration Public Meeting, Washington, DC | NOAA Gulf Spill

Science.gov (United States)

benefit injured marshes, coastal dune and nearshore habitats, oysters, and human uses (on water recreation . Department of Commerce Herbert Hoover Building Auditorium 1401 Constitution Ave., NW Washington, D.C. 20230

Psychiatric Boarding in Washington State and the Inadequacy of Mental Health Resources.

Science.gov (United States)

Bloom, Joseph D

2015-06-01

Psychiatric boarding is a term derived from emergency medicine that describes the holding of patients deemed in need of hospitalization in emergency departments for extended periods because psychiatric beds are not available. Such boarding has occurred for many years in the shadows of mental health care as both inpatient beds and community services have decreased. This article focuses on a 2014 Washington State Supreme Court decision that examined the interpretation of certain sections of the Washington state civil commitment statute that had been used to justify the extended boarding of detained psychiatric patients in general hospital emergency departments. The impact of this decision on the state of Washington should be significant and could spark a national debate about the negative impacts of psychiatric boarding on patients and on the nation's general hospital emergency services. © 2015 American Academy of Psychiatry and the Law.

Ebb and Flow: Maintaining the Close Air Support Relationship through History

Science.gov (United States)

2016-05-10

3-09.3, Close Air Support (Washington, DC: Government Printing Office, 2014), xi. 20 Scott Fischer , LtCol, USAF, “Army and Air Force Subcultures...also happens that airmen and ground forces can 30 Jeff Schogol, "Welsh: The Air Force cares about...Advanced Military Studies, 2004), 59. 60 Fischer , “Army Air Force Subcultures,” 15. 61 Bolton, “Army Fixed-Wing Attack,” 5-6. 21 external

78 FR 26249 - Drawbridge Operation Regulation; Lake Washington Ship Canal, Seattle, WA

Science.gov (United States)

2013-05-06

...The Coast Guard has issued a temporary deviation from the operating schedule that governs the Montlake Bridge across the Lake Washington Ship Canal, mile 5.2, at Seattle, WA, and the University Bridge across the Lake Washington Ship Canal, mile 4.3, at Seattle, WA. This deviation is necessary to accommodate the ``Beat the Bridge'' foot race. This deviation allows the bridges to remain in the closed position to allow safe movement of event participants.

Port Angeles, Washington Tsunami Forecast Grids for MOST Model

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The Port Angeles, Washington Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST)...

Timber resource statistics for eastern Washington, 1995.

Science.gov (United States)

Neil McKay; Patricia M. Bassett; Colin D. MacLean

1995-01-01

This report summarizes a 1990-91 timber resource inventory of Washington east of the crest of the Cascade Range. The inventory was conducted on all private and public lands except National Forests. Timber resource statistics from National Forest inventories also are presented. Detailed tables provide estimates of forest area, timber volume, growth, mortality, and...

A angústia dos corpos indóceis: prostituição e conflito armado na Colômbia contemporânea The anguish of indocile bodies: prostitution and the armed conflict in contemporary Colombia

OpenAIRE

José Miguel Nieto Olivar

2008-01-01

Uma das estratégias de controle usadas pelos grupos armados no marco da agudização do conflito na Colômbia é o controle e a "gerência" da prostituição (e da sexualidade em geral) nos territórios de dominação. O que significa ser prostituta no contexto de controle armado masculino na Colômbia contemporânea? Quais as possibilidades da vivência dos direitos humanos nas mulheres trabalhadoras sexuais nesse contexto? Essas perguntas surgem da vivência próxima da história da Lady entre os anos 2003...

78 FR 74163 - Harrison Medical Center, a Subsidiary of Franciscan Health System Bremerton, Washington; Notice...

Science.gov (United States)

2013-12-10

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-83,070] Harrison Medical Center, a Subsidiary of Franciscan Health System Bremerton, Washington; Notice of Negative Determination... workers of Harrison Medical Center, a subsidiary of Franciscan Health System, Bremerton, Washington...

Neah Bay, Washington Tsunami Forecast Grids for MOST Model

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The Neah Bay, Washington Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model....

Toke Point, Washington Tsunami Forecast Grids for MOST Model

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The Toke Point, Washington Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model....

La Push, Washington Tsunami Forecast Grids for MOST Model

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The La Push, Washington Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model....

77 FR 51563 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

Science.gov (United States)

2012-08-24

... Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA AGENCY: National... Anthropology, has completed an inventory of human remains and associated funerary objects, in consultation with... of Washington, Department of Anthropology. Disposition of the human remains and associated funerary...

78 FR 5198 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

Science.gov (United States)

2013-01-24

... Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA AGENCY: National... Anthropology, has completed an inventory of human remains and associated funerary objects, in consultation with... of Washington, Department of Anthropology. Disposition of the human remains to the Indian tribes...

76 FR 73664 - Notice of Inventory Completion: Washington State University, Museum of Anthropology, Pullman, WA

Science.gov (United States)

2011-11-29

...: Washington State University, Museum of Anthropology, Pullman, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Washington State University, Museum of Anthropology (WSU) has completed an... University, Museum of Anthropology, Pullman, WA 99164-4910, telephone (509) 335-4314. SUPPLEMENTARY...

Volatile substance misuse deaths in Washington State, 2003-2012.

Science.gov (United States)

Ossiander, Eric M

2015-01-01

Volatile substance misuse (VSM - also known as huffing or sniffing) causes some deaths, but because there are no specific cause-of-death codes for VSM, these deaths are rarely tabulated. Count and describe VSM deaths occurring in Washington State during 2003-2012. We used the textual cause-of-death information on death certificates to count VSM-associated deaths that occurred in Washington State during 2003-2012. We extracted records that contained words suggesting either a method of inhalation or a substance commonly used for VSM, and reviewed those records to identify deaths on which the inhalation of a volatile substance was mentioned. We conducted a descriptive analysis of those deaths. Fifty-six deaths involving VSM occurred in Washington State during 2003-2012. VSM deaths occurred primarily among adults age 20 and over (91%), males (88%), and whites (93%). Twelve different chemicals were associated with deaths, but 1 of them, difluoroethane, was named on 30 death certificates (54%), and its involvement increased during the study period. Gas duster products were named as the source of difluoroethane for 12 deaths; no source was named for the other 18 difluoroethane deaths. Most VSM deaths occurred among white male adults, and gas duster products containing difluoroethane were the primary source of inhalants. Approaches to deter VSM, such as the addition of bitterants to gas dusters, should be explored.

A trajetória de Padre Luiz Sponchiado na região da Quarta Colônia = The path of Priest Luiz Sponchiado in Fourth Colony region/RS/Brazil

Directory of Open Access Journals (Sweden)

Manfio, Juliana Maria

2014-01-01

Full Text Available O Padre Luiz Sponchiado (1922-2010 nasceu e atuou em região oriunda de colonização italiana, conhecida como Quarta Colônia, localizada no centro do Rio Grande do Sul. O presente trabalho tem por interesse a compreensão do cenário que se estabeleceu por trás do religioso, principalmente os campos político e cultural, para melhor compreender as ações desempenhadas pelo padre em concomitância com a vida religiosa. Padre Luiz atuou no eixo das mobilizações políticas, como a luta pela municipalização dos antigos núcleos coloniais em um único município: a Quarta Colônia. Porém tal realização não deu certo devido às disparidades de cada núcleo e resultaram, tempos depois, na emancipação de cada núcleo. Padre Luiz agiu ainda no campo cultural, através da criação de um importante acervo, o Centro de Pesquisas Genealógicas e, a partir dele, buscou a (resignificação da Quarta Colônia. Para isso, propagou o discurso do imigrante italiano e católico, difundindo entre a população a ideia da “identidade italiana”. Nesse sentido, as ações políticas e culturais extrapolaram o campo religioso de um pároco

O Crisol: periódico das alunas do Colégio Americano (Porto Alegre/RS, 1945-1964 - O Crisol: the jornal by students of the Colégio Americano (Porto Alegre/RS, 1945-1964

Directory of Open Access Journals (Sweden)

Dóris Bittencourt Almeida, Brasil

2013-04-01

Full Text Available Neste trabalho investiga-se o periódico O Crisol, produzido pelas alunas do Colégio Americano, uma escola de Porto Alegre/RS, entre os anos de 1945 e 1964. Esse periódicodiz muito à História da Educação, pois evidencia saberes e práticas que circulavam nacomunidade do Colégio e revela um pouco das representações da cultura juvenil daquele tempo. Aqui interessam os significados dos discursos difundidos pelo periódico nos processos de subjetivação provocados pelos textos e suas possíveis influências na construção das identidades das jovens leitoras e escritoras do jornal. A pesquisa identifica-se com os pressupostos teóricos da História Cultural e se inscreve no campo das práticas de leitura e escrita e de memórias juvenis, tendo como referenciais as concepções da cultura escrita enquanto produção discursiva de um determinado tempo e lugar.Palavras-chave: memórias juvenis, escritas escolares, práticas de escrita. O CRISOL: THE JORNAL BY STUDENTS OF THE COLÉGIO AMERICANO (PORTO ALEGRE/RS, 1945-1964 AbstractThis work investigates the journalO Crisolproduced by students of the Colégio Americano, a school in Porto Alegre/RS, between the years 1945 and 1964.The journal says much to the History of Education, as evidenced knowledges and pratices circulating in the community,showing a bit of representations of youth culture in that time. Herethe interest by meaning’s dissemination of speeches by the journal in the processes of subjectivation caused by texts and their possible influences on the construction of identities of young readers and writers of the newspaper. The research identifies with the theoreticals presuppositions o Cultural History and inscribed in the field of reading and writing practices and memories of youth, taking as reference the concepts of the writing culture as a discursive production of a determinated time and place.Key-words: youth’s memories, written school, writing practices. O CRISOL: EL

Breve nota sobre o II Colóquio Brasil-Japão (Tóquio, 29 de fevereiro - 7 de março de 1968

Directory of Open Access Journals (Sweden)

Maria Regina da Cunha Rodrigues

1968-03-01

Full Text Available Êsse Colóquio, continuação do I realizado em São Paulo de 25 a 27 de julho de 1966, cujos Anais foram objeto de recente publicação (*, ao lado do saldo positivo que conclaves dêsse tipo alcançam, apresentou algumas falhas que, com a devida vênia, mencionaremos a seguir.

Alternative Fuels Data Center: Washington Transportation Data for

Science.gov (United States)

stations in Washington with alternative fuels Fuel Public Private Biodiesel (B20 and above) 8 33 Compressed Partnerships Spark Biodiesel Success for Essential Baking Company Partnerships Spark Biodiesel Success for Videos on YouTube Video thumbnail for Seattle Bakery Delivers With Biodiesel Trucks Seattle Bakery

Dignity, death, and dilemmas: a study of Washington hospices and physician-assisted death.

Science.gov (United States)

Campbell, Courtney S; Black, Margaret A

2014-01-01

The legalization of physician-assisted death in states such as Washington and Oregon has presented defining ethical issues for hospice programs because up to 90% of terminally ill patients who use the state-regulated procedure to end their lives are enrolled in hospice care. The authors recently partnered with the Washington State Hospice and Palliative Care Organization to examine the policies developed by individual hospice programs on program and staff participation in the Washington Death with Dignity Act. This article sets a national and local context for the discussion of hospice involvement in physician-assisted death, summarizes the content of hospice policies in Washington State, and presents an analysis of these findings. The study reveals meaningful differences among hospice programs about the integrity and identity of hospice and hospice care, leading to different policies, values, understandings of the medical procedure, and caregiving practices. In particular, the authors found differences 1) in the language used by hospices to refer to the Washington statute that reflect differences among national organizations, 2) the values that hospice programs draw on to support their policies, 3) dilemmas created by requests by patients for hospice staff to be present at a patient's death, and 4) five primary levels of noninvolvement and participation by hospice programs in requests from patients for physician-assisted death. This analysis concludes with a framework of questions for developing a comprehensive hospice policy on involvement in physician-assisted death and to assist national, state, local, and personal reflection. Copyright © 2014 U.S. Cancer Pain Relief Committee. Published by Elsevier Inc. All rights reserved.

Cristóbal Colón como puente trasatlántico en el imaginario de Eugenio María de Hostos

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Rodríguez de Laguna, Asela

2015-12-01

Full Text Available Since Eugenio María de Hostos’ publication in 1863 of La peregrinación de Bayoán until 1892, Columbus occupies a central space in his writings. The transformation of Columbus from a Romantic to a Positivist hero serves to exhibit the ideological, historical and political tensions of a colonial Puerto Rican subject questioning his historical and political legacy. It also triggers the examination not only of the figure of Columbus, Spain and the conquest, but of the Nineteenth century complex reality of the American, Caribbean and Latin American nation states.Desde la publicación de La peregrinación de Bayoán (1863 hasta el 1892 la escritura de Eugenio María de Hostos evidencia un especial interés por la figura de Cristóbal Colón. La centralidad del Descubridor, así como su transformación de genio romántico a positivista en el discurso hostosiano pone de relieve las tensiones históricas, políticas e ideológicas de un sujeto puertorriqueño colonial con respecto a su legado histórico-político. Sirve para cuestionar no solo la figura del mismo Colón, sino la conquista, y la situación de los pueblos de las dos Américas en el siglo diecinueve.

78 FR 25471 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

Science.gov (United States)

2013-05-01

....R50000] Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA..., Department of Anthropology, has completed an inventory of human remains and associated funerary objects, in... Burke Museum acting on behalf of the University of Washington, Department of Anthropology. Disposition...

78 FR 5200 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

Science.gov (United States)

2013-01-24

... Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA AGENCY: National... Anthropology, has completed an inventory of human remains and associated funerary objects, in consultation with... acting on behalf of the University of Washington, Department of Anthropology. Disposition of the human...

78 FR 19297 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

Science.gov (United States)

2013-03-29

...-PPWOCRADN0] Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA..., Department of Anthropology, has completed an inventory of human remains and associated funerary objects, in... Burke Museum acting on behalf of the University of Washington, Department of Anthropology. Disposition...

77 FR 74871 - Notice of Inventory Completion: The Museum of Anthropology at Washington State University...

Science.gov (United States)

2012-12-18

... Inventory Completion: The Museum of Anthropology at Washington State University, Pullman, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Museum of Anthropology has completed an... objects may contact the Museum of Anthropology at Washington State University. Repatriation of the human...

78 FR 2432 - Notice of Inventory Completion: The Museum of Anthropology at Washington State University...

Science.gov (United States)

2013-01-11

... Inventory Completion: The Museum of Anthropology at Washington State University, Pullman, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Museum of Anthropology has completed an... objects may contact the Museum of Anthropology at Washington State University. Repatriation of the human...

78 FR 44594 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

Science.gov (United States)

2013-07-24

..., 2013. ADDRESSES: Alicia Woods, Washington State Parks and Recreation Commission, PO Box 42650, Olympia, WA 98504-2650, telephone (360) 902- 0939, email Alicia[email protected] . SUPPLEMENTARY INFORMATION... to Alicia Woods, Washington State Parks and Recreation Commission, PO Box 42650, Olympia, WA 98504...

Osteoporosis and Related Genes: VDR, ESR And COL1A1

Directory of Open Access Journals (Sweden)

Sabriye Kocaturk Sel

2011-08-01

Full Text Available Osteoporosis is now considered as one of the major and growing health care problems around the world. Osteoporosis is the most prevalent metabolic bone disease among developed countries and it is defined as a systemic skeletal disease characterized by low bone mass and microarchitectural deterioration of bone tissue with a consequent increase in bone fragility. Bone is a highly metabolically active tissue in which the processes of osteoblastic bone formation and osteoclastic bone resorption are continuous throughout life. Coupling of osteoblast and osteoclast action ensures that a normal bone structure is maintained. A loss of bone homeostasis may result in a decrease in bone mass leading to osteoporosis or in a defect in the mineralization of bone. Numerous genetic, hormonal, nutritional and life-style factors contribute to the acquisition and maintenance of bone mass. Among them, genetic variations explain as much as 50-80% of the variance for bone mineral density (BMD in the population. Many genes that could be related to osteoporosis have been studied and of them all Vitamin D receptor (VDR, estrogen receptor alpha (ESRα and collagen 1 alpha 1 chain (COL1A1 genes have been the most focused on. [Archives Medical Review Journal 2011; 20(4.000: 246-269

Ehlers-Danlos Syndrome type IV: a multi-exon deletion in one of the two COL3A1 alleles affecting structure, stability, and processing of type III procollagen

International Nuclear Information System (INIS)

Superti-Furga, A.; Gugler, E.; Gitzelmann, R.; Steinmann, B.

1988-01-01

The authors have studied a patient with severe, dominantly inherited Ehlers-Danlos syndrome type IV. The results indicate that this patient carries a deletion of 3.3 kilobase pairs in the triple helical coding domain of one of the two alleles for the pro-α-chains of type III collagen (COL3A1). His cultured skin fibroblasts contain equal amounts of normal length mRNA and of mRNA shortened by approximately 600 bases, and synthesize both normal and shortened pro-α1(III)-chains. In procollagen molecules containing one or more shortened chains, a triple helix is formed with a length of only about 780 amino acids. The mutant procollagen molecules have decreased thermal stability, are less efficiently secreted, and are not processed as their normal counterpart. The deletion in this family is the first mutation to be described in COL3A1

Washington Conference

International Nuclear Information System (INIS)

Anon.

1981-01-01

The 1981 Particle Accelerator Conference was held in Washington from 11-13 March. It was the ninth in the series of meetings organized in the USA which differ from the 'International' meetings in their coverage of the full range of accelerator engineering and technology, including applications outside e field of high energy physics. The Conference took place under the cloud of further budget cuts for Fiscal Year 1982 in the USA which the Department of Energy has applied in line with the financial policy of the new administration. Coming on top of many years of budget trimming which have reduced the number of high energy physics Laboratories funded by the DOE to three (Brookhaven, Fermilab, Stanford - Cornell is funded by the National Science Foundation) and reduced the exploitation of these Laboratories to less than half of their potential, the new cuts did not exactly help to boost morale. Nevertheless, the huge amount of tailed work in accelerator physics and technology which was presented at the Conference showed how alive the field is

VizieR Online Data Catalog: The Washington Visual Double Star Catalog (Mason+ 2001-2014)

Science.gov (United States)

Mason, B. D.; Wycoff, G. L.; Hartkopf, W. I.; Douglass, G. G.; Worley, C. E.

2018-05-01

The Washington Visual Double Star Catalog (WDS) is the successor to the Index Catalogue of Visual Double Stars, 1961.0 (IDS; Jeffers and van den Bos, Publ. Lick Obs. 21). Three earlier double star catalogs in XXth century, those by Burnham (BDS, 1906, "General Catalogue of Double Stars within 121 degrees of the North Pole", Carnegie Institution of Washington), Innes (SDS, 1927, "Southern Double Star Catalogue -19 to -90 degrees", Union Observatory, Johannesburg, South Africa), and Aitken (ADS, 1932 "New General Catalogue of Double Stars within 121 degrees of the North Pole", Carnegie Institution of Washington), each covered only a portion of the sky. Both the IDS and the WDS cover the entire sky, and the WDS is intended to contain all known visual double stars for which at least one differential measure has been published. The WDS is continually updated as published data become available. Prior to this, three major updates have been published (Worley and Douglass 1984, "Washington Visual Double Star Catalog, 1984.0", U.S. Naval Observatory, Washington; Worley and Douglass 1997A&AS..125..523W, Cat. I/237; Mason, Wycoff, Hartkopf, Douglass and Worley 2001AJ....122.3466M; and Mason et al. 2006.5). The Washington Double Star Catalog (WDS) has seen numerous changes since the last major release of the catalog. The application of many techniques and considerable industry over the past few years has yielded significant gains in both the number of systems and the number of measures. Is is maintained by the US Naval Observatory, and represents the world's principal database of astrometric double and multiple star information. The WDS contains positions (J2000), discoverer designations, epochs, position angles, separations, magnitudes, spectral types, proper motions, and, when available, Durchmusterung numbers and notes for the components of the systems. (3 data files).

Washington Wildlife Mitigation Projects : Final Programmatic Environmental Assessment and Finding of No Significant Impact.

Energy Technology Data Exchange (ETDEWEB)

United States. Bonneville Power Administration; Washington (State). Dept. of Fish and Wildlife.

1996-08-01

Bonneville Power Administration (BPA) proposes to fund the portion of the Washington Wildlife Mitigation Agreement (Agreement) pertaining to wildlife habitat mitigation projects to be undertaken in a cooperative effort with the Washington Department of Fish and Wildlife (WDFW). This Agreement serves to establish a monetary budget funded by BPA for projects proposed by Washington Wildlife Coalition members and approved by BPA to protect, mitigate, and improve wildlife and/or wildlife habitat within the State of Washington that has been affected by the construction of Federal dams along the Columbia River. This Environmental Assessment examines the potential environmental effects of acquiring and/or improving wildlife habitat within five different project areas. These project areas are located throughout Grant County and in parts of Okanogan, Douglas, Adams, Franklin, Kittias, Yakima, and Benton Counties. The multiple projects would involve varying combinations of five proposed site-specific activities (habitat improvement, operation and maintenance, monitoring and evaluation, access and recreation management, and cultural resource management). All required Federal, State, and tribal coordination, permits and/or approvals would be obtained prior to ground-disturbing activities.

Washington wildlife mitigation projects. Final programmatic environmental assessment and finding of no significant impact

International Nuclear Information System (INIS)

1996-08-01

Bonneville Power Administration (BPA) proposes to fund the portion of the Washington Wildlife Mitigation Agreement (Agreement) pertaining to wildlife habitat mitigation projects to be undertaken in a cooperative effort with the Washington Department of Fish and Wildlife (WDFW). This Agreement serves to establish a monetary budget funded by BPA for projects proposed by Washington Wildlife Coalition members and approved by BPA to protect, mitigate, and improve wildlife and/or wildlife habitat within the State of Washington that has been affected by the construction of Federal dams along the Columbia River. This Environmental Assessment examines the potential environmental effects of acquiring and/or improving wildlife habitat within five different project areas. These project areas are located throughout Grant County and in parts of Okanogan, Douglas, Adams, Franklin, Kittias, Yakima, and Benton Counties. The multiple projects would involve varying combinations of five proposed site-specific activities (habitat improvement, operation and maintenance, monitoring and evaluation, access and recreation management, and cultural resource management). All required Federal, State, and tribal coordination, permits and/or approvals would be obtained prior to ground-disturbing activities

78 FR 19298 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

Science.gov (United States)

2013-03-29

...-PPWOCRADN0] Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA..., Department of Anthropology, has completed an inventory of human remains, in consultation with the appropriate... Washington, Department of Anthropology. Disposition of the human remains to the Indian tribes stated below...

78 FR 22286 - Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA

Science.gov (United States)

2013-04-15

....R50000] Notice of Inventory Completion: University of Washington, Department of Anthropology, Seattle, WA..., Department of Anthropology, has completed an inventory of human remains, in consultation with the appropriate... acting on behalf of the University of Washington, Department of Anthropology. If no additional requestors...

78 FR 45958 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2013-07-30

... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-13406; PPWOCRADN0-PCU00RP14.R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke...

78 FR 59963 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2013-09-30

... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-13770; PPWOCRADN0-PCU00RP14.R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of..., Interior. ACTION: Notice. SUMMARY: The Thomas Burke Memorial Washington State Museum, University of...

78 FR 44595 - Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of...

Science.gov (United States)

2013-07-24

... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-13407; PPWOCRADN0-PCU00RP14.R50000] Notice of Inventory Completion: Thomas Burke Memorial Washington State Museum, University of Washington, Seattle, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Thomas Burke...

Experience with Honeycrisp apple storage management in Washington

Science.gov (United States)

High demand and premium pricing have led to rapid increases in Honeycrisp plantings and fruit volume in Washington State, USA since introduction of the cultivar in 1999. Most fruit is packed and sold by January because of strong demand coupled with difficulties associated with extended storage. Howe...

De gota em gota: violência, tempo e troca em Urabá, Colômbia

Directory of Open Access Journals (Sweden)

Silvia Monroy

2014-12-01

Full Text Available Comparo, neste texto, a modalidade de empréstimo de dinheiro imposta pelos "gota a gota" com a minha experiência como voluntária no Banco de la Esperanza,em Urabá, considerada uma das regiões mais violentas da Colômbia durante, pelo menos, quatro décadas. Mediante este contraste, passo a analisar a relação entre o que denomino "presente permanente" e determinados aspectos da troca, a saber: "o costume de ficar devendo", "a necessidade de pedir emprestado", e o imperativo de que "tudo deve ser dado". No caso estudado, tais efeitos são aspectos primordiais da vida coletiva e da reprodução social. Busco, em particular, explorar a relação entre violência e "economia", vínculo que costuma ser relegado ao campo da ilegalidade.

Analisis TLC entre Colombia y Estados Unidos: Estados de Virginia y Washington

OpenAIRE

Pulecio Leon, Gloria Daniela

2016-01-01

el punto central de esta investigación será identificar las principales fortalezas y oportunidades de las empresas colombianas frente al Tratado de Libre Comercio con Estados Unidos, con miras al desarrollo de un proceso de exportación no tradicional e internacionalización, en los estados de Virginia y Washington. Para esto debemos primero reconocer las oportunidades que el mercado colombiano ha aprovechado en los estados de Virginia y Washington, segundo revisar si el tratado de libre ...

Status of birds at the Hanford Site in southeastern Washington

International Nuclear Information System (INIS)

Landeen, D.S.; Johnson, A.R.; Mitchell, R.M.

1992-06-01

The US Department of Energy has entered into agreements with the Washington State Department of Ecology, the US Environmental Protection Agency, and Hanford Site contractors to focus work activities on cleanup and stabilization of radioactive and hazardous waste sites located at the Hanford Site in southeastern Washington. Ecological characterization is an essential part of the remediation process, and the identification of biotic components such as bird species that could be impacted by cleanup activities is an important part of the initial environmental characterizations. Site characterization work has resulted in this list of 238 birds that have been observed at the Hanford Site. This list is presented with a status rating for abundance and seasonal occurrence

Autógrafos de Cristobal Colón: Ensayo de Análisis Grafológico de su Escritura

Directory of Open Access Journals (Sweden)

Boletín Cultural y Bibliográfico Banco de la República

1968-02-01

Full Text Available Absolutamente el único mapa o dibujo topográfico que resta de Colón. De este marino, que toda su vida confeccionó mapas, que de modo seguro tuvo que haber dibujado las derrotas de sus viajes, que se sabe que regaló un gran mapa a Isabel, solo queda este pedazo de papel (hoy en el Archivo de la Casa de Alba, en Madrid, "misteriosamente adquirido por la Duquesa de Alba", según Thacher (I, p. 477, quien lo reproduce en (III, p. 8 9 y dice : "Evidentemente dibujado en enero de 1493.

Genetic variation in steelhead (Salmo gairdneri) from the north coast of Washington

Science.gov (United States)

Reisenbichler, R.R.; Phelps, S.R.

1989-01-01

Steelhead (Salmo gairdneri) collected from various sites in nine drainages in northwestern Washington were genetically characterized at 65 protein-coding loci by starch-gel electrophoresis. Genetic differentiation within and among drainages was not significant, and genetic variation among drainages was much less than that reported in British Columbia; these results may be the consequence of gene flow from hatchery stocks that have been released in Washington since the 1940's. Allele frequencies varied significantly among year-classes (hence, genetic characterization studies must include data from several year-classes), and also between hatchery fish (including a stock developed with local wild fish) and wild fish, indicating that few wild fish have been successfully and routinely included in hatchery brood stocks. Conservation of genetic diversity along the north coast of Washington should be facilitated by reducing the numbers of hatchery fish that spawn in streams and by including wild fish in hatchery brood stocks.

78 FR 50099 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

Science.gov (United States)

2013-08-16

... at the address in this notice by September 16, 2013. ADDRESSES: Alicia Woods, Washington State Parks... Alicia[email protected] . SUPPLEMENTARY INFORMATION: Notice is here given in accordance with the Native... request Alicia Woods, Washington State Parks and Recreation Commission, PO Box 42650, Olympia, WA 98504...

78 FR 44593 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

Science.gov (United States)

2013-07-24

...: Alicia Woods, Washington State Parks and Recreation Commission, PO Box 42650, Olympia, WA 98504-2650, telephone (360) 902- 0939, email Alicia[email protected] . SUPPLEMENTARY INFORMATION: Notice is here given... written request with information in support of the request to Alicia Woods, Washington State Parks and...

University of Washington Center for Child Environmental Health Risks Research

Data.gov (United States)

Federal Laboratory Consortium — The theme of the University of Washington based Center for Child Environmental Health Risks Research (CHC) is understanding the biochemical, molecular and exposure...

Security, Violent Events, and Anticipated Surge Capabilities of Emergency Departments in Washington State

Science.gov (United States)

Weyand, Jonathan S.; Junck, Emily; Kang, Christopher S.; Heiner, Jason D.

2017-01-01

Introduction Over the past 15 years, violent threats and acts against hospital patients, staff, and providers have increased and escalated. The leading area for violence is the emergency department (ED) given its 24/7 operations, role in patient care, admissions gateway, and center for influxes during acute surge events. This investigation had three objectives: to assess the current security of Washington State EDs; to estimate the prevalence of and response to threats and violence in Washington State EDs; and to appraise the Washington State ED security capability to respond to acute influxes of patients, bystanders, and media during acute surge events. Methods A voluntary, blinded, 28-question Web-based survey developed by emergency physicians was electronically delivered to all 87 Washington State ED directors in January 2013. We evaluated responses by descriptive statistical analyses. Results Analyses occurred after 90% (78/87) of ED directors responded. Annual censuses of the EDs ranged from violent threats or acts occurring in their ED. Of these, 93% were directed towards nursing staff, 90% towards physicians, 74% towards security personnel, and 51% towards administrative personnel. Nearly half (48%) noted incidents directed towards another patient, and 50% towards a patient’s family or friend. These events were variably reported to the hospital administration. After an acute surge event, 35% believed the initial additional security response would not be adequate, with 26% reporting no additional security would be available within 15 minutes. Conclusion Our study reveals the variability of ED security staffing and a heterogeneity of capabilities throughout Washington State. These deficiencies and vulnerabilities highlight the need for other EDs and regional emergency preparedness planners to conduct their own readiness assessments. PMID:28435498

Unmet Student Financial Need in the State of Washington: A Study of the "Need Gap."

Science.gov (United States)

Fenske, Robert; And Others

A study of unmet student financial need in Washington State was conducted by the Washington Council for Postsecondary Education. "Unmet need" is the difference between need and the total amount of aid received by the student through federal, state, and institutional financial aid programs, privately funded scholarships, and nonsubsidized…

Five egg-laying queens in a single colony of brazilian stingless bees (Melipona scutellaris Latreille Presença de cinco rainhas fisogástricas em colônia de abelha sem ferrão (Melipona scutellaris Latreille

Directory of Open Access Journals (Sweden)

Carlos Alfredo Lopes de Carvalho

2011-03-01

Full Text Available Polygyny, characterized by the presence of several egg-laying queens, is considered as a temporary colony status. In stingless bees it is rarely observed. This paper reports the first case of natural polygyny in Melipona scutellaris colony, with five egg-laying queens.Poliginia, caracterizada pela presença de mais de uma rainha poedeira, é considerada como uma condição temporária em colônias. Em abelhas sem ferrão isso é raramente observado. Este artigo registra o primeiro caso de poliginia natural em colônia de Melipona scutellaris, com cinco rainhas poedeiras.

A practical method for screening for beta-galactosidase secreting microbial colonies Método prático de triagem de colônias de microrganismos secretoras de beta-galactosidase

Directory of Open Access Journals (Sweden)

Eriana S. Barreto

2000-03-01

Full Text Available Microbial colonies were replicated on YNB® agar plates overlaid with soft agar containing the glucose-oxidase/peroxidase (BIOTROL® system. The pink color developed around the colonies was the result of the reaction of the glucose generated by the extracellular hydrolysis of lactose by beta-galactosidase, indicating secretion of this enzyme. This method proved to be very convenient for testing hundreds of colonies grown on agar plates for beta-galactosidase secretion by microbial cells.Colônias microbianas foram replicadas na superfície de ágar YNB® contendo lactose, recoberta com uma camada de ágar semi-sólido contendo o sistema glicose-oxidase/peroxidase (BIOTROL®. Coloração rosa foi desenvolvida ao redor das colônias como resultado da reação de glicose gerada pela hidrólise extracelular de lactose pela beta-galactosidase, indicando secreção da enzima pelo microrganismo.

Avian use of proposed KENETECH and CARES wind farm sites in Klickitat County, Washington. Appendix C to Washington Windplant No. 1 EIS. Technical report

International Nuclear Information System (INIS)

1995-01-01

The Columbia Hills area above (north of) the Columbia River in Klickitat County, in southcentral Washington, is being considered for development of two wind power generation projects that could include the eventual placement of up to 436 wind turbines. The KENETECH Windpower Washington Windplant TM Number 1 project would include placing up to 345 KENETECH 33M-VS turbines, capable of producing up to 115 megawatts (MW), in 39 rows (strings) on a 5,110-hectare (12,630-acre) site. During scoping for these proposed developments, concerns were raised regarding the potential for avian mortality associated with wind farm development. Collision with wind turbine blade, towers, guy wires, and transmission lines, and electrocution from power lines have been identified as sources of avian mortality, particularly raptors, at existing wind farm facilities. To address these concerns, an avian study was conducted at the site in accordance with an avian study plan and protocol developed, with input from a national avian task force, state agencies (Washington Department of Fish and Wildlife [WDFW]), and federal agencies (USFWS). The study included four elements: (1) a winter raptor and waterfowl study, (2) spring migration and fall migration studies, (3) a summer resident study, and (4) a raptor breeding study. The study involved extensive field studies conducted by biologists experienced in identifying raptors and other birds

Hello Mr. President! Rollenspiele zwischen Hollywood und Washington

NARCIS (Netherlands)

Mehring, F.

2016-01-01

Hello Mr. President Rollenspiele zwischen Hollywood und Washington Am 8. November 2016 entscheidet sich, wer zum 58. Präsidenten der USA gewählt wird: Die ehemalige Außenministerin Hillary Clinton oder der republikanische Kandidat Donald Trump. In der heißen Phase des Wahlkampfs fällt besonders

Radiological Survey Results for Areas A1 North, A5A, A6, and B2 at the Molycorp Washington Remediation Project, Washington, Pennsylvania

International Nuclear Information System (INIS)

W.C. Adams

2007-01-01

Perform radiological surveys of the Molycorp Washington Remediation Project (MWRP) facility in Washington, Pennsylvania. The U.S. Nuclear Regulatory Commission (NRC) requested the Oak Ridge Institute for Science and Education (ORISE) to provide limited training pertaining to ORISE radiological soil scanning and sampling procedures to the Pennsylvania Department of Environmental Protection (PADEP) personnel. In addition, the NRC also requested that ORISE perform radiological surveys of the Molycorp Washington Remediation Project (MWRP) facility in Washington, Pennsylvania (Figure 1). ORISE has and will continue to interface with PADEP personnel in a joint effort to perform confirmatory radiological surveys, consisting of gamma scans and soil sampling, at the MWRP facility. PADEP personnel will continue to submit soil samples that they collect to ORISE for analyses. PADEP sample results, along with ORISE results, will be provided to the NRC and PADEP so that decisions regarding the radiological status of the surveyed areas can be determined. ORISE performed radiological surveys during the period of November 28 and 29, 2006. The survey unit (SU) available for ORISE radiological survey activities was Area A1 North. The MWRP final status survey (FSS) results for Area A1 North were reviewed prior to these survey activities. Prior to ORISE's survey activities, PADEP personnel had performed radiological surveys and collected soil samples from SU Areas A5A and A6. These samples were provided to ORISE for analyses while on site. After the ORISE radiological surveys, PADEP personnel collected soil samples from SU Areas A2 and B2 and these samples were shipped to ORISE for analyses in January 2007. Figure 2 depicts the MWRP Areas A through D; ORISE and PADEP personnel performed survey activities in portions of Areas A and B. For interlaboratory comparison analyses with MWRP's site contractor, Malcolm Pirnie (MP), ORISE requested soil samples from the Area A1 soil stockpiles

Parent's Guide to Special Education in Washington State, 1985-86 [and] Guia para Padres: Para Educacion Especial en el Estado de Washington.

Science.gov (United States)

Washington Office of the State Superintendent of Public Instruction, Olympia.

This pamphlet guides parents of children with disabilities through the procedures for acquiring special education services in the state of Washington. Following an overview of special education, the pamphlet presents information on notice and consent procedures, confidentiality of records, individualized education programs (IEP), the placement…

Confirmation of the pathogenicity of a mutation p.G337C in the COL1A2 gene associated with osteogenesis imperfecta

Science.gov (United States)

Jia, Mingrui; Shi, Ranran; Zhao, Xuli; Fu, Zhijian; Bai, Zhijing; Sun, Tao; Zhao, Xuejun; Wang, Wenbo; Xu, Chao; Yan, Fang

2017-01-01

Abstract Mutation analysis as the gold standard is particularly important in diagnosis of osteogenesis imperfecta (OI) and it may be preventable upon early diagnosis. In this study, we aimed to analyze the clinical and genetic materials of an OI pedigree as well as to confirm the deleterious property of the mutation. A pedigree with OI was identified. All family members received careful clinical examinations and blood was drawn for genetic analyses. Genes implicated in OI were screened for mutation. The function and structure of the mutant protein were predicted using bioinformatics analysis. The proband, a 9-month fetus, showed abnormal sonographic images. Disproportionately short and triangular face with blue sclera was noticed at birth. She can barely walk and suffered multiple fractures till 2-year old. Her mother appeared small stature, frequent fractures, blue sclera, and deformity of extremities. A heterozygous missense mutation c.1009G>T (p.G337C) in the COL1A2 gene was identified in her mother and her. Bioinformatics analysis showed p.G337 was well-conserved among multiple species and the mutation probably changed the structure and damaged the function of collagen. We suggest that the mutation p.G337C in the COL1A2 gene is pathogenic for OI by affecting the protein structure and the function of collagen. PMID:28953610

siRNA-mediated Allele-specific Silencing of a COL6A3 Mutation in a Cellular Model of Dominant Ullrich Muscular Dystrophy

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Véronique Bolduc

2014-01-01

Full Text Available Congenital muscular dystrophy type Ullrich (UCMD is a severe disorder of early childhood onset for which currently there is no effective treatment. UCMD commonly is caused by dominant-negative mutations in the genes coding for collagen type VI, a major microfibrillar component of the extracellular matrix surrounding the muscle fibers. To explore RNA interference (RNAi as a potential therapy for UCMD, we designed a series of small interfering RNA (siRNA oligos that specifically target the most common mutations resulting in skipping of exon 16 in the COL6A3 gene and tested them in UCMD-derived dermal fibroblasts. Transcript analysis by semiquantitative and quantitative reverse transcriptase PCR showed that two of these siRNAs were the most allele-specific, i.e., they efficiently knocked down the expression from the mutant allele, without affecting the normal allele. In HEK293T cells, these siRNAs selectively suppressed protein expression from a reporter construct carrying the mutation, with no or minimal suppression of the wild-type (WT construct, suggesting that collagen VI protein levels are as also reduced in an allele-specific manner. Furthermore, we found that treating UCMD fibroblasts with these siRNAs considerably improved the quantity and quality of the collagen VI matrix, as assessed by confocal microscopy. Our current study establishes RNAi as a promising molecular approach for treating dominant COL6-related dystrophies.

Inimkatsed viisid eestlased Washington Posti esiküljele / Neeme Raud

Index Scriptorium Estoniae

Raud, Neeme, 1969-

2000-01-01

Eilne Washington Post kirjutas esiküljelt alanud artiklis Lääne farmaatsiagigantide kuritegelikest inimkatsetest, kasutades ühe näitena Postimehes ilmunud ülevaadet Šveitsi ravimifirma katsetest noorte eestlastega

Timber resource statistics for Washington, January 1, 1973.

Science.gov (United States)

Patricia M. Bassett; Grover A. Choate

1974-01-01

Timber resource statistics to January 1, 1973, for the State of Washington show total land area, commercial timberland area, and growing stock and sawtimber inventory volumes by county and owner group. Growth and removals are shown by Forest Survey inventory unit for 1972. Each National Forest is updated to January 1, 1973.

Clash of the Titans: Booker T. Washington and W. E. B. Du Bois. Curriculum Based Education Program, Grade 11.

Science.gov (United States)

National Park Service (Dept. of Interior), Washington, DC.

The Booker T. Washington National Monument preserves and protects the birth site and childhood home of Booker T. Washington while interpreting his life experiences and significance in U.S. history as the most powerful African American between 1895 and 1915. The programs and activities included in this guide about the Booker T. Washington and W. E.…

Effect in field of the native strain COL6 of Rhizobium leguminosarum bv. viciae on common bean cv. Percal in Ecuador

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Klever Iván Granda-Mora

2017-07-01

Full Text Available Inoculation with selected Rhizobium strains of common bean (Phaseolus vulgaris L. contributes to the agricultural yield of this legume, improves soil fertility and reduces the use of nitrogen fertilizers in sustainable agricultural systems. The research aimed to determine the effect in field of the native strain Rhizobium leguminosarum bv. viciae COL6 on growth, N fixation and agricultural yield in common bean cv. Percal. A field trial was carried out to evaluate the parameters of nodulation, biomass, nitrogen fixation, yield components and agricultural yield in common bean. In addition, soil analyzes were performed before and after experimentation. Prior to planting an inoculant was formulated with the strain COL6. The seeds of cv. Percal were mixed in 250 g inoculant per 10 kg of seed and it was sown manually in the soil. The results of growth, N fixation (total N % and agricultural yield demonstrated the positive effect of inoculation with the bacterial inoculant comparable with chemical fertilizers. The increase in agricultural yield with bacterial inoculant application and chemical fertilization with respect to control was 62 and 64 %, respectively. The results of the soil analysis were variable with respect to the initial physical-chemical characteristics. The promising results of bacterial inoculant use and the effective stimulation in common bean, open the doors for its potential use in Ecuadorian soils and with that the desired potential yield can be reached.

Final Report: Feasibility Study of Biomass in Snohomish County, Washington

Energy Technology Data Exchange (ETDEWEB)

Daryl Williams (Tulalip Tribes); Ray Clark (Clark Group)

2005-01-31

This report and its attachments summarizes the results of a unique tribal-farmer cooperative study to evaluate the feasibility of building one or more regional anaerobic digestion systems in Snohomish County, Washington.

Variação sazonal das condições internas de colônias de Melipona marginata obscurior Moure, no Rio Grande do Sul, Brasil Seasonal variations in the internal condictions of colonies the Melipona marginata obscurior Moure, at Rio Grande do Sul, Brazil

Directory of Open Access Journals (Sweden)

Francine Von B. Borges

2006-09-01

Full Text Available O conhecimento das condições internas das colônias de Melipona marginata obscurior Moure, 1971, ao longo do ano, é importante para a compreensão do comportamento das abelhas sem ferrão e seu manejo. Visa-se o estudo da biologia de M. marginata obscurior em ambiente natural, com ênfase na variação sazonal das condições internas das colônias. Quatro colônias foram estudadas no Centro de Pesquisas e Conservação da Natureza Pró-Mata, em São Francisco de Paula, RS. Avaliou-se o volume dos ninhos, a ocorrência e duração da diapausa e o número total de favos e de células de cria nos dois últimos favos. O volume do invólucro inicial das quatro colônias foi semelhante, variando de 2,26 a 2,69 litros. Ao final da avaliação observou-se que o volume do invólucro da colônia A reduziu 5%, enquanto as demais cresceram 61% (colônia B, 84% (C e 71% (D. A partir do monitoramento mensal dos favos de cria constatou-se a ocorrência de diapausa em três colônias (B, C e D enquanto a colônia A permaneceu com postura ininterruptamente. As colônias cessaram a construção de células de cria e postura da rainha durante o outono e reiniciaram esta atividade no final do inverno. Sugere-se que a variação do período de diapausa (8 a 23 semanas seja determinada por fatores internos das colônias. O número máximo de favos registrado durante a primavera e o verão variou de 6 a 8, enquanto que o número estimado de células de cria dos dois últimos favos variou de 116 a 1190. Foi possível reconhecer padrões, bem como variações sazonais, relacionados ao volume do invólucro dos ninhos, à ocorrência de diapausa e ao número de favos e de células de cria nos dois últimos favos. Variações sazonais das condições internas entre as colônias mostraram-se evidentes e podem ser creditadas a características intrínsecas das colônias.The knowledge of the internal conditions of Melipona marginata obscurior Moure, 1971 colonies, through

77 FR 72907 - Western Washington Railroad, LLC-Lease and Operation Exemption-City of Tacoma, Department of...

Science.gov (United States)

2012-12-06

..., 2012, Western Washington Railroad, LLC (WWRR), a Washington limited liability company and noncarrier... allow for interchange with WWRR, BNSF Railway Company, the Puget Sound and Pacific Railroad, and Union Pacific Railroad Company, and also over the entire line for emergency routing. WWRR states that the...

Uso da terra e perda de solo na Bacia Hidrográfica do Rio Colônia, Bahia

OpenAIRE

Silva,Vinícius de A.; Moreau,Mauricio S.; Moreau,Ana M. S. dos S.; Rego,Neylor A. C.

2011-01-01

Mudanças no uso da terra muitas vezes potencializam a erosão hídrica acarretando perda de água, solo, nutriente e matéria orgânica dos sistemas agrícolas, razão por que se estimou a perda de solo na bacia hidrográfica do rio Colônia, na Bahia, nos últimos vinte e sete anos, utilizando-se o software SWAT (Soil and Water Assessment Tool). Para tal, procedeu-se à digitalização de mapas temáticos, interpretação de fotografias aéreas de 1975; classificação supervisionada de imagens de satélites de...

Routine environmental audit of the Hanford Site, Richland, Washington

Energy Technology Data Exchange (ETDEWEB)

1994-05-01

This report documents the results of the routine environmental audit of the Hanford Site (Hanford), Richland, Washington. During this audit, the activities conducted by the audit team included reviews of internal documents an reports from previous audits and assessments; interviews with US Department of Energy (DOE), State of Washington regulatory, and contractor personnel; and inspections and observations of selected facilities and operations. The onsite portion of the audit was conducted May 2--13, 1994, by the DOE Office of Environmental Audit (EH-24), located within the Office of Environment, Safety and Health (EH). The audit evaluated the status of programs to ensure compliance with Federal, State, and local environmental laws and regulations; compliance with DOE orders, guidance, and directives; and conformance with accepted industry practices and standards of performance. The audit also evaluated the status and adequacy of the management systems developed to address environmental requirements.

Routine environmental audit of the Hanford Site, Richland, Washington

International Nuclear Information System (INIS)

1994-05-01

This report documents the results of the routine environmental audit of the Hanford Site (Hanford), Richland, Washington. During this audit, the activities conducted by the audit team included reviews of internal documents an reports from previous audits and assessments; interviews with US Department of Energy (DOE), State of Washington regulatory, and contractor personnel; and inspections and observations of selected facilities and operations. The onsite portion of the audit was conducted May 2--13, 1994, by the DOE Office of Environmental Audit (EH-24), located within the Office of Environment, Safety and Health (EH). The audit evaluated the status of programs to ensure compliance with Federal, State, and local environmental laws and regulations; compliance with DOE orders, guidance, and directives; and conformance with accepted industry practices and standards of performance. The audit also evaluated the status and adequacy of the management systems developed to address environmental requirements

The State of Washington's Children. [Seventh Annual Report].

Science.gov (United States)

Marvinney, Sandy, Ed.

This KIDS COUNT seventh annual report examines statewide trends in the well-being of Washington's children. The statistical portrait is based 24 key indicators of well-being: (1) teen birth rate; (2) teen pregnancy rate; (3) births to unmarried mothers; (4) divorces involving children; (5) family foster caseload; (6) average real wages; (7) per…

Detección de proteínas de adhesión a fibronectina y colágeno presentes en Leptospira interrogans serovar Canicola

Directory of Open Access Journals (Sweden)

Gisele Reyes

2007-12-01

Full Text Available Como parte de los estudios encaminados a la obtención de una formulación vacunal por subunidades contra la leptospirosis humana, se describe la purificación y caracterización de las proteínas de unión a fibronectina y a colágeno en Leptospira interrogans. Las proteínas de la membrana externa fueron extraídas mediante la solubilización con Tritón X-114 y se aplicaron en una columna de afinidad de IgG AntiBSA-Sepharose 2B CL, para eliminar la BSA, contaminante principal del medio de cultivo en que crece el microorganismo. La muestra libre de BSA (no fijado se aplicó a una columna de afinidad de fibronectina Sepharose 4B-CNBr, que permitió la separación y detección de una fracción que contenía una proteína de unión a fibronectina presente en la cepa 87 de Leptospira interrogans serovar Canicola, cuyo peso molecular fue estimado en 40 kDa. La proteína aislada demostró ser antigénica y conservada en los serovares Canicola, Copenhageni y Mozdok, en el ensayo de inmunodetección utilizado en este estudio (Dot blot. Para ello se utilizaron sueros específicos obtenidos en ratas infectadas experimentalmente con cada serovar y una mezcla de sueros de humanos convalecientes de leptospirosis. Las proteínas de membrana externa solubilizadas con Tritón X-114, libres de BSA, fueron aplicadas también a una columna de afinidad colágeno-Sepharosa 4B-CNBr, que permitió la purificación de una proteína de unión a colágeno con un peso molecular de aproximadamente 25 kDa, la cual resultó ser antigénica frente a sueros de humanos convalecientes de la enfermedad. Ambas proteínas seleccionadas (40 kD y 25 kD podrían ser evaluadas como posibles inmunógenos en futuros estudios encaminados a la obtención de nuevos antígenos vacunales.

75 FR 10442 - Sweet Cherries Grown in Designated Counties in Washington; Change in the Handling Regulation

Science.gov (United States)

2010-03-08

.... The existing paragraph (e) would be redesignated as paragraph (d), and the introductory sentence of... reported by the National Agricultural Statistics Service, USDA, and 1,500 Washington cherry producers, the... revise the introductory sentence of paragraph (g) to read as follows: Sec. 923.322 Washington cherry...

José de Anchieta, o teatro e a educação dos moços do Colégio de Jesus na Bahia do século XVI

Directory of Open Access Journals (Sweden)

Paulo Romualdo Hernandes

2012-12-01

Full Text Available Este estudo trata do teatro educativo jesuítico no Brasil do século XVI. Aborda o teatro deAnchieta e tem como objeto específico de pesquisa a peça “Diálogo de Pero Dias”, queteria sido apresentada no Colégio de Jesus na ciudad do Salvador, na Bahia. Um auto nafórmula de diálogo, por meio de perguntas e respostas, como o catecismo tridentino,preparadas para a catequese. Diálogo que teria como espectadores, provavelmente, oscolonos, viajantes, homens do governo, soldados, mas como alvo principal de seusensinamentos os “moços” do colégio. O objetivo deste estudo é fazer uma nova leituradessa peça de Anchieta para resgatar, a partir de uma leitura crítica baseada em Foucault,os ensinamentos que os padres jesuítas pretendiam para os “moços”, mas também paraoutras personagens sociais do Brasil do século XVI.

Composition at Washington State University: Building a Multimodal Bricolage

Science.gov (United States)

Ericsson, Patricia; Hunter, Leeann Downing; Macklin, Tialitha Michelle; Edwards, Elizabeth Sue

2016-01-01

Multimodal pedagogy is increasingly accepted among composition scholars. However, putting such pedagogy into practice presents significant challenges. In this profile of Washington State University's first-year composition program, we suggest a multi-vocal and multi-theoretical approach to addressing the challenges of multimodal pedagogy. Patricia…

Booker T. Washington's Audacious Vocationalist Philosophy

Science.gov (United States)

Lewis, Theodore

2014-01-01

Booker T. Washington was born a slave in the American South, rising remarkably in the period after slavery to become a leader of his race. His advocacy of appeasement with the Southern white establishment incurred the ire of his black peers, given the withdrawal of the franchise from ex-slaves in southern states after a brief period of positive…

Greenhouse gas mitigation options for Washington State

Energy Technology Data Exchange (ETDEWEB)

Garcia, N.

1996-04-01

President Clinton, in 1993, established a goal for the United States to return emissions of greenhouse gases to 1990 levels by the year 2000. One effort established to help meet this goal was a three part Environmental Protection Agency state grant program. Washington State completed part one of this program with the release of the 1990 greenhouse gas emissions inventory and 2010 projected inventory. This document completes part two by detailing alternative greenhouse gas mitigation options. In part three of the program EPA, working in partnership with the States, may help fund innovative greenhouse gas reduction strategies. The greenhouse gas control options analyzed in this report have a wide range of greenhouse gas reductions, costs, and implementation requirements. In order to select and implement a prudent mix of control strategies, policy makers need to have some notion of the potential change in climate, the consequences of that change and the uncertainties contained therein. By understanding the risks of climate change, policy makers can better balance the use of scarce public resources for concerns that are immediate and present against those that affect future generations. Therefore, prior to analyzing alternative greenhouse gas control measures, this report briefly describes the phenomenon and uncertainties of global climate change, and then projects the likely consequences for Washington state.

Washington State University Algae Biofuels Research

Energy Technology Data Exchange (ETDEWEB)

chen, Shulin [Washington State Univ., Pullman, WA (United States). Dept. of Biological Systems Engineering; McCormick, Margaret [Targeted Growth, Inc., Seattle, WA (United States); Sutterlin, Rusty [Inventure Renewables, Inc., Gig Harbor, WA (United States)

2012-12-29

The goal of this project was to advance algal technologies for the production of biofuels and biochemicals by establishing the Washington State Algae Alliance, a collaboration partnership among two private companies (Targeted Growth, Inc. (TGI), Inventure Chemicals (Inventure) Inc (now Inventure Renewables Inc) and Washington State University (WSU). This project included three major components. The first one was strain development at TGI by genetically engineering cyanobacteria to yield high levels of lipid and other specialty chemicals. The second component was developing an algal culture system at WSU to produce algal biomass as biofuel feedstock year-round in the northern states of the United States. This system included two cultivation modes, the first one was a phototrophic process and the second a heterotrophic process. The phototrophic process would be used for algae production in open ponds during warm seasons; the heterotrophic process would be used in cold seasons so that year-round production of algal lipid would be possible. In warm seasons the heterotrophic process would also produce algal seeds to be used in the phototrophic culture process. Selected strains of green algae and cyanobacteria developed by TGI were tested in the system. The third component was downstream algal biomass processing by Inventure that included efficiently harvesting the usable fuel fractions from the algae mass and effectively isolating and separating the usable components into specific fractions, and converting isolated fractions into green chemicals.

Quantification of left ventricular myocardial collagen system in children, young adults, and the elderly Cuantificación del sistema de colágeno del ventrículo izquierdo del miocardio en niños, adultos jóvenes y ancianos

Directory of Open Access Journals (Sweden)

Andrea Bogoslavsky Levy Mendes

2012-06-01

Full Text Available Studies on the collagen system of the human myocardium are still limited compared to those on small laboratory animals. The aim of this work was to observe the collagen tissue of the myocardium of the human heart as a function of age. The types of collagen, as well as the density of collagen tissue and the diameter of collagen fibrils, were examined. Fragments of the left ventricular wall from 15 hearts, 5 from children, 5 from young adults, and 5 from elderly individuals, were analyzed by using the Picrosirius-polarization method and by transmission electron microscopy (TEM. The results showed the presence of collagen type III and collagen type I, both in the endomysium and perimysium of the 3 groups studied. Measurements of collagen content in myocardial tissue displayed that both endomysial and perimysial collagen increase in number and thickness in the adult and elderly. These histochemical results coincided with the observations obtained with the electron microscope in showing an increase in the number of collagen fibrils with a large diameter in the adult and elderly hearts. The present results on cardiac collagen may be important for assessing the pathogenesis of several cardiopathies in the hearts of children, young adults, and the elderly.Los estudios sobre el colágeno del miocardio humano son aún escasos en comparación con los hechos en pequeños animales de laboratorio. El objetivo de este trabajo fue cuantificar el tejido colágeno del miocardio del corazón humano en función de la edad. Se estudiaron los tipos de colágeno, su densidad y el diámetro de las fibrillas de colágeno. Para esto se utilizaron fragmentos de la pared del ventrículo izquierdo de 15 corazones, cinco de niños, cinco de adultos jóvenes y 5 de personas de edad avanzada. Las muestras se analizaron mediante el método de Picrosirius-polarización y por microscopía electrónica de transmisión (MET. Los resultados mostraron la presencia de colágeno tipo

Surface hydrologic investigations of the Columbia Plateau Region, Washington

International Nuclear Information System (INIS)

Leonhart, L.S.

1979-07-01

The Washington State portion of the Columbia Plateau is divided into six hydrologic sub-basins on the basis of the principal surface drainage systems present, structural and topographic relationships, and political and other considerations. Baseline descriptions of the surface water systems and resources are presented for the Columbia Plateau with emphasis on the Pasco Sub-basin. A preliminary evaluation of the hydrologic budget for each sub-basin is derived. For each sub-basin, recharge/discharge relationships arising from precipitation/evapotranspiration/runoff, stream losses and gains, and artificial mechanisms are determined on the basis of available data. The net exchange between surface and groundwater systems is evaluated and relative estimates of the net groundwater flow into or out of the sub-basin are obtained. An evaluation is made of hydrologic risk factors arising from: (1) tributary flooding in eastern Washington; and, (2) major flooding of the Columbia River within the Pasco Sub-basin. Scenarios are presented for credible natural and man-generated catastrophic events

Surface hydrologic investigations of the Columbia Plateau region, Washington

International Nuclear Information System (INIS)

Leonhart, L.S.

1979-01-01

The Washington State portion of the Columbia Plateau is divided into six hydrologic sub-basins on the basis of the principal surface drainage systems present, structural and topographic relationships, and political and other considerations. Baseline descriptions of the surface water systems and resources are presented for the Columbia Plateau with emphasis on the Pasco Sub-basin. A preliminary evaluation of the hydrologic budget for each sub-basin is derived. For each sub-basin, recharge/discharge relationships arising from precipitation/evapotranspiration/runoff, stream losses and gains, and artificial mechanisms are determined on the basis of available data. The net exchange between surface and ground-water systems is evaluated and relative estimates of the net ground-water flow into or out of the sub-basin are obtained. An evaluation is made of hydrologic risk factors arising from: (1) tributary flooding in eastern Washington; and (2) major flooding of the Columbia River within the Pasco Sub-basin. Scenarios are presented for credible natural and man-generated catastrophic events

Walter Reed Army Medical Center, Washington, D. C. - Annual Progress Report FY-89. Volume 1

Science.gov (United States)

1990-01-02

Enemas Cause Hyperphosphatemia and Acidemia in Patients with Ulcerative Colitis After Routine5 Colonic Cleansing." 4. LCDR Michael S. Opsahl, MC...Cholestyramine Treatment of 101 Thyrotoxicosis (3/88) 1303-88 Burman, Kenneth COL MC. The Clinical Application of In Situ 102 Hybridization to Detect Viral...Absorption of Commercial 144 (Oral) Calcium Carbonate Supplements (10/87) 1398-87 Solomon, Barbara DNSc. The Management of Hyperthyroidism Due 145 to

An integrated DEA-COLS-SFA algorithm for optimization and policy making of electricity distribution units

International Nuclear Information System (INIS)

Azadeh, A.; Ghaderi, S.F.; Omrani, H.; Eivazy, H.

2009-01-01

This paper presents an integrated data envelopment analysis (DEA)-corrected ordinary least squares (COLS)-stochastic frontier analysis (SFA)-principal component analysis (PCA)-numerical taxonomy (NT) algorithm for performance assessment, optimization and policy making of electricity distribution units. Previous studies have generally used input-output DEA models for benchmarking and evaluation of electricity distribution units. However, this study proposes an integrated flexible approach to measure the rank and choose the best version of the DEA method for optimization and policy making purposes. It covers both static and dynamic aspects of information environment due to involvement of SFA which is finally compared with the best DEA model through the Spearman correlation technique. The integrated approach would yield in improved ranking and optimization of electricity distribution systems. To illustrate the usability and reliability of the proposed algorithm, 38 electricity distribution units in Iran have been considered, ranked and optimized by the proposed algorithm of this study.

Solutions for Dioctyl Phthalate (DOP) tested high efficiency particulate air (HEPA) filters destined for disposal at Hanford, Washington

International Nuclear Information System (INIS)

Gablin, K.A.

1992-11-01

In January 1992, Argonne National Laboratory East, Environmental and Waste Management Program, learned that a chemical material used for testing of all HEPA filters at the primary source, Flanders Filter, Inc. in Washington, NC, was considered a hazardous chemical by Washington State Dangerous Waste Regulations. These regulations are under the jurisdiction of the Washington Administration Code, Chapter 173-303, and therefore directly under impact the Hanford Site Solid Waste Acceptance Criteria. Dioctyl Phthalate, ''DOP'' as it is referred to in chemical abbreviation form, is added in small test quantities at the factory, at three Department of Energy (DOE) operated HEPA filter test facilities, and in the installed duct work at various operating laboratories or production facilities. When small amounts of radioactivity are added to the filter media in operation, the result is a mixed waste. This definition would normally only develop in the state of Washington since their acceptance criteria is ten times more stringent then the US Environmental Protection Agencys' (US EPA). Methods of Processing will be discussed, which will include detoxification, physical separation, heat and vacuum separation, and compaction. The economic impact of a mixed waste definition in the State of Washington, and an Low Level Waste (LLW) definition in other locations, may lend this product to be a prime candidate for commercial disposal in the future, or a possible de-listing by the State of Washington

The DNA-mimic antirestriction proteins ArdA ColIB-P9, Arn T4, and Ocr T7 as activators of H-NS-dependent gene transcription.

Science.gov (United States)

Melkina, Olga E; Goryanin, Ignatiy I; Zavilgelsky, Gennadii B

2016-11-01

The antirestriction proteins ArdA ColIb-P9, Arn T4 and Ocr T7 specifically inhibit type I and type IV restriction enzymes and belong to the family of DNA-mimic proteins because their three-dimensional structure is similar to the double-helical B-form DNA. It is proposed that the DNA-mimic proteins are able to bind nucleoid protein H-NS and alleviate H-NS-silencing of the transcription of bacterial genes. Escherichia coli lux biosensors were constructed by inserting H-NS-dependent promoters into a vector, thereby placing each fragment upstream of the promoterless Photorhabdus luminescens luxCDABE operon. It was demonstrated that the DNA-mimic proteins ArdA, Arn and Ocr activate the transcription of H-NS-dependent promoters of the lux operon of marine luminescent bacteria (mesophilic Aliivibrio fischeri and psychrophilic Aliivibrio logei), and the dps gene from E. coli. It was also demonstrated that the ArdA antirestriction protein, the genes of which are located on transmissive plasmids ColIb-P9, R64, PK101, decreases levels of H-NS silencing of the PluxC promoter during conjugation in the recipient bacteria. Copyright © 2016 Elsevier GmbH. All rights reserved.

Despertant la curiositat cientìfica en Educació Infantil a través de la col·laboració de la familia, la escola i el centre universitari

Directory of Open Access Journals (Sweden)

José Hidalgo-Navarrete

2012-01-01

Full Text Available L’experiència es planteja com una activitat per a l’alumnat d’Educació Infantil. La fi és despertar la curiositat científica i fomentar la col·laboració entre els membres de la comunitat educativa pel fet que hi participen pares i mares de l’alumnat, estudiants d’Educació Infantil i professors de l’Escola Universitària. El desenvolupament de l’experiència es va basar en l’organització d’una jornada de tallers per a l’alumnat de segon cicle d’Educació Infantil del CEIP «Sant Ginés de la Jara», de Sabiote (Jaén, en els quals es presentava el descobriment de fenòmens científics a través d’experiments, amb la col·laboració dels pares i mares interessats en el projecte i de l’alumnat de Magisteri de l’Escola Universitària de Magisteri «Sagrada Família», d’Úbeda, propera al centre en el qual es va desenvolupar l’experiència. Es van dur a terme un total de quatre tallers basats en una metodologia científica, deixant als nens que observessin en primer lloc, fessin hipòtesis, comprovessin el fenomen sobre el qual estaven fent les conjectures i, finalment, extraguessin una conclusió. Per estudiar la iniciació a la curiositat científica en el grup de nens i nenes d’infantil utilitzem la tècnica qualitativa de producció de textos. Així mateix, com l’experiència es va preparar com un projecte de formació a través del treball col·laboratiu de diversos sectors de la comunitat, ens vam proposar estudiar l’evolució en la interrelació de teoria i pràctica entre l’alumnat de magisteri a través d’un disseny d’investigació d’un sol grup amb pretest-postest i a partir d’un qüestionari de resolució de situacions científiques problemàtiques. De manera similar, per analitzar si el context familiar constituïa un entorn de col·laboració per encoratjar la curiositat científica i contribuir des d’un altre ambient diferent a l’escolar al seu desenvolupament, utilitzem un

Análise histomorfométrica dos cardiomiócitos e deposição de colágeno no músculo cardíaco de ratas ooforectomizadas

Directory of Open Access Journals (Sweden)

Maria C.R. Silva

2016-03-01

Full Text Available Resumo: Esse estudo objetivou avaliar o efeito da ooforectomia sob os parâmetros histomorfométricos dos cardiomiócitos e quantificação de colágeno em ratas Wistar. Vinte ratas foram utilizadas e separadas em dois grupos: GI - ooforectomizada e GII - sham-ooforectomizada. Após a recuperação anestésica os animais foram acomodados em gaiolas e separados nas condições de ciclo claro/escuro 12/12h por um período de 6 meses. Passado o período experimental, os animais foram anestesiados, o coração retirado e mergulhado em formaldeído a 10%. Os fragmentos dos ventrículos foram submetidos ao processamento histológico e corados com hematoxilina e eosina. As análises histomorfométricas (área celular e área e volume nuclear foram realizadas pelo microscópio de luz e software ImageJ versão 1.44. Os dados foram submetidos à análise ANOVA e quando significantes, complementados pelo teste t de student (p<0,001. Observou-se uma diminuição significativa das áreas celular e nuclear dos cardiomiócitos do grupo GI quando comparado ao grupo GII, bem como a área ocupada, pelo colágeno, no músculo cardíaco foi maior em GI, quando comparado a GII. Diante disso, conclui-se que as alterações observadas nos cardiomiócitos do grupo GI, sugerem uma maior atividade apoptótica nesse grupo, devido à diminuição dos níveis séricos do estrógeno provocado pela ooforectomia e o aumento da área ocupada pelo colágeno, no grupo ooforectomizado, está associada ao efeito cardioprotetor do estrógeno.

Aerial radiological survey of the Washington. Date of survey: July 1982 Public Power Supply System (WPPSS) Nuclear Project and surrounding area, Richland, Washington

International Nuclear Information System (INIS)

1983-07-01

An aerial radiological survey was performed from 14 to 20 July 1982 over a 270-square-kilometer area centered on the Washington Public Power Supply System (WPPSS) Nuclear Project located near Richland, Washington. All gamma ray data were collected by flying parallel lines spaed 152 meters (500 feet) apart at an altitude of 91 meters (300 feet) above ground level. Count rates obtained from the aerial platform were converted to total external exposure rates at 1 meter above the ground and are presented in the form of an isoradiation contour map. The observed exposure rates ranged from 5 to 15 microroentgens per hour (μR/h) with the average background ranging from 9 to 12μR/h. These values include an estimated cosmic ray contribution of 3.7 μR/h. The exposure rates obtained from ground-based measurements taken in background locations within the survey area displayed positive agreement the aerial data

Relationship of infant and fetal mortality to operations at the Hanford Nuclear Reservation, Washington State, 1946-1982

International Nuclear Information System (INIS)

Cate, S.; Hansom, J.

1986-01-01

The relationship of infant and fetal mortality to numbers of nuclear reactors at the Hanford Nuclear Reservation was investigated. Mortality rates were obtained using 36 years of United States vital statistics data. Three different exposure groups were selected based on meteorologic studies of the Hanford area: group 1, counties downwind of Hanford all year; group 2, counties seasonally downwind; and group 3, counties not downwind. Washington state was used as an additional comparison group. Four periods of operation based on fluctuations in numbers of reactors were characterized. Log-linear analysis revealed that the three groups and Washington state had similar trends in infant mortality rates over the four time periods. On the other hand, the trend in fetal mortality rates for group 1 did differ significantly from trends for the two other groups and Washington state. The trends of fetal mortality rates for group 2, group 3, and Washington state were not statistically different. Fetal mortality rates in group 1, however, failed to decline from period 1 (1946-1954) to period 2 (1955-1964) as expected by the trends for the two groups and Washington state. During period 2, the greatest number of reactors were operating. County-specific analysis showed that, of the counties in group 1, the trend in fetal mortality for Benton County, where Hanford is located, was significantly different from that for Washington state. A possible link between Hanford and an excess in fetal deaths is suggested by the deviation in trend of group 1, which appears localized to Benton County and the period of peak activity at Hanford

Metro de Washington EE.UU.

Directory of Open Access Journals (Sweden)

Weese, Harry

1979-09-01

Full Text Available This article describes the works involved in the first stage of the Washington Underground (Subway system which was begun in 1969 and scheduled for completion in 1983 and is the most modern metropolitan railway in North America. Lines have double track and will carry three million passengers daily. Different construction methods have been used throughout: tunnel formed try digging a trench then roofed and covered, excavated tunnel and elevated structures. Stations features answer to the strictest demands, provided with closed circuit television, air conditioning, noise dampening Systems, special access ways, fire protection Systems and automatic traffic control. Special attention is given to the two bridges over the Pentagon and over the Anacostia, pointing out their differences and the elevated structure at the National Airport.

Se describen en este articulo los trabajos de la primera fase del Metro de Washington que, iniciado en el año 1969 será, a su terminación en el año 1983, el más moderno sistema de ferrocarril metropolitano de Norte América. Es de doble carril y servirá para tres millones de usuarios. Se han empleado distintos sistemas de obra en su realización: túnel artificial realizado mediante una zanja que después se cubre; túnel perforado, y estructuras aéreas. Las características de las estaciones responden a las mayores exigencias, pues tienen circuito cerrado de televisión, aire acondicionado, sistemas para atenuar el ruido, accesos especiales, sistema de protección contra el fuego y control automático del Metro. Se estudian de un modo particular: los dos puentes sobre el Pentágono y el Anacostia, señalando sus diferencias y la estructura aérea del Aeropuerto Nacional.

Produção e desenvolvimento de colônias de abelhas africanizadas (Apis mellifera l. a partir de diferentes áreas e idades de cria Production and development of africanized honey bee (Apis mellifera l. colonies departing from different comb brood areas and brood ages

Directory of Open Access Journals (Sweden)

Roberto Henrique Dias da Silva

2004-04-01

Full Text Available A apicultura brasileira usa da captura de enxames silvestres de abelhas melíferas africanizadas (Apis mellifera L. para repor e/ou aumentar o número de colônias dos apiários, possuindo inconvenientes como a dependência da natureza para captura dos enxames, a heterogeneidade genética das colônias capturadas e a possibilidade desses enxames serem portadores de doenças e parasitas prejudiciais à sanidade das abelhas. O presente trabalho testa e apresenta uma técnica de divisão de colônias de abelhas melíferas africanizadas para a produção de novas colônias fortes em curto espaço de tempo, a partir de recursos mínimos de cera, cria e alimento. Os resultados mostraram que núcleos de A. mellifera formados inicialmente com uma rainha jovem e fecundada, 1 kg de operárias, um quadro de cria fechada, um quadro de favo puxado e vazio e dois quadros com cera alveolada permitem a produção de novas colônias em 42 dias. Portanto, pode-se concluir que a técnica de divisão de colônias por formação de núcleos como descrito acima, oferece aos apicultores uma alternativa viável para a produção e comercialização em larga escala de novas colônias de abelhas melíferas africanizadas.The Brazilian apiculture relies upon collecting wild swarms of Africanized honey bees (Apis mellifera L. to replace and/or increase the number of colonies in the apiaries. This practice brings problems such as dependence on nature to capture any swarm, diverse genetic make-up of the colonies captured and the possibility of these swarms be carrying diseases and parasites harmful to the bees. The present work tests and presents a technique to split colonies of Africanized honey bees to produce new strong colonies in short time, departing from little resources of wax, brood and food stores. Results showed that A. mellifera nuclei formed by a young and mated queen, 1kg of workers, a frame of sealed brood, an empty frame of drawn beeswax and two frames

Presença de cinco rainhas fisogástricas em colônia de abelha sem ferrão (Melipona scutellaris Latreille)

OpenAIRE

Carvalho, Carlos Alfredo Lopes de; Souza, Bruno de Almeida; Dias, Carleandro de Souza; Alves, Rogério Marcos de Oliveira; Melo, Alex Fábio de Lima; Soares, Ana Cristina Fermino; Carvalho-Zilse, Gislene Almeida

2011-01-01

Polygyny, characterized by the presence of several egg-laying queens, is considered as a temporary colony status. In stingless bees it is rarely observed. This paper reports the first case of natural polygyny in Melipona scutellaris colony, with five egg-laying queens.Poliginia, caracterizada pela presença de mais de uma rainha poedeira, é considerada como uma condição temporária em colônias. Em abelhas sem ferrão isso é raramente observado. Este artigo registra o primeiro caso de poliginia n...

Feasibility of irradiating Washington fruits and vegetables for Asian export markets

Energy Technology Data Exchange (ETDEWEB)

Eakin, D.E.; Hazelton, R.F.; Young, J.K.; Prenguber, B.A.; O' Rourke, A.D.; Heim, M.N.

1987-05-01

US agricultural export marketing opportunities are limited by the existence of trade barriers in many overseas countries. For example, Japan and South Korea do not permit the importation of apples due to their stated concern over codling moth infestation. One of the purposes of this study was to evaluate the potential of exporting irradiated fruits and vegetables from Washington State to overcome existing trade barriers and prevent the establishment of future barriers. The Asian countries specifically evaluated in this study are Japan, Hong Kong and Singapore. Another purpose of this project was to determine the feasibility of locating an irradiation facility in Washington State. Advantages that irradiated agricultural products would bring in terms of price and quality in export markets were also evaluated.

Monomer-dimer control of the ColE1 P(cer) promoter.

Science.gov (United States)

Chatwin, H M; Summers, D K

2001-11-01

XerCD-mediated recombination at cer converts multimers of plasmid ColE1 to monomers, maximizing the number of independently segregating molecules and minimizing the frequency of plasmid loss. In addition to XerCD, recombination requires the accessory factors ArgR and PepA. The promoter P(cer), located centrally within cer, is also required for stable plasmid maintenance. P(cer) is active in plasmid multimers and directs transcription of a short RNA, Rcd, which appears to inhibit cell division. It has been proposed that Rcd is part of a checkpoint which ensures that multimer resolution is complete before the cell divides. This study has shown that ArgR does not act as a transcriptional repressor of P(cer) in plasmid monomers. P(cer) is unusual in that the -35 and -10 hexamers are separated by only 15 bp and this study has demonstrated that increasing this to a more conventional spacing results in elevated activity. An increase to 17 bp resulted in a 10- to 20-fold increase in activity, while smaller effects were seen when the spacer was increased to 16 bp or 18 bp. These observations are consistent with the hypothesis that P(cer) activation involves realignment of the -35 and -10 sequences within a recombinational synaptic complex. This predicts that a 17 bp spacer promoter derivative should be down-regulated by plasmid multimerization, and this is confirmed experimentally.

Digital Learning Compass: Distance Education State Almanac 2017. Washington

Science.gov (United States)

Seaman, Julia E.; Seaman, Jeff

2017-01-01

This brief report uses data collected under the U.S. Department of Education's National Center for Educational Statistics (NCES) Integrated Postsecondary Education Data System (IPEDS) Fall Enrollment survey to highlight distance education data in the state of Washington. The sample for this analysis is comprised of all active, degree-granting…

Snag Dynamics in Western Oregon and Washington

OpenAIRE

Ohmann, Janet L

2002-01-01

To achieve desired amounts and characteristics of snags and down wood, managers require analytical tools for projecting changes in dead wood over time, and for comparing those changes to management objectives such as providing dead wood for wildlife and ecosystem processes. The following information on rates of snag recruitment, decay, and fall across forests of western Oregon and Washington may be useful in planning for future levels of dead wood. Eventually the information will be incorpora...

Immobilization of cross linked Col-I–OPN bone matrix protein on aminolysed PCL surfaces enhances initial biocompatibility of human adipogenic mesenchymal stem cells (hADMSC)

Energy Technology Data Exchange (ETDEWEB)

Kim, Young-Hee; Jyoti, Md. Anirban; Song, Ho-Yeon, E-mail: songmic@sch.ac.kr

2014-06-01

In bone tissue engineering surface modification is considered as one of the important ways of fabricating successful biocompatible material. Addition of biologically active functionality on the surfaces has been tried for improving the overall biocompatibility of the system. In this study poly-ε-caprolactone film surfaces have been modified through aminolysis and immobilization process. Collagen type I (COL-I) and osteopontin (OPN), which play an important role in osteogenesis, was immobilized onto PCL films followed by aminolysis treatment using 1,6-hexanediamine. Characterization of animolysed and immobilized surfaces were done by a number techniques using scanning electron microscopy (SEM), FT-IR, XPS, ninhydrin staining, SDS-PAGE and confocal microscopy and compared between the modified and un-modified surfaces. Results of the successive experiments showed that aminolysis treatment was homogeneously achieved which helped to entrap or immobilize Col-I–OPN proteins on surfaces of PCL film. In vitro studies with human adipogenic mesenchymal stem cells (hADMSC) also confirmed the attachment and proliferation of cells was better in modified PCL surfaces than the unmodified surfaces. SEM, confocal microscopy and MTT assay showed a significant increase in cell spreading, attachment and proliferations on the biofunctionalized surfaces compared to the unmodified PCL surfaces at all-time points indicating the success of surface biofunctionalization.

75 FR 33736 - Tart Cherries Grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington...

Science.gov (United States)

2010-06-15

...., Stop 0237, Washington, DC 20250-0237; telephone: (202) 720-2491, Fax: (202) 720-8938, or E-mail: Laurel... DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 930 [Doc. No. AO-370-A8; AMS..., Oregon, Utah, Washington, and Wisconsin; Withdrawal of Proposed Rule AGENCY: Agricultural Marketing...

Aerial Photography and Imagery, Ortho-Corrected - 2007 Digital Orthophotos - Washington County

Data.gov (United States)

NSGIC Education | GIS Inventory — This dataset is a collection of GeoTIFF and MrSID format natural color orthophotos covering Washington, Holmes, and Bay County, Florida. An orthophoto is remotely...

Aerial Photography and Imagery, Ortho-Corrected - 2012 Digital Orthophotos - Washington County

Data.gov (United States)

NSGIC Education | GIS Inventory — This dataset is a collection of GeoTIFF and MrSID format natural color orthophotos covering Holmes and Washington County, Florida. An orthophoto is remotely sensed...

Aerial Photography and Imagery, Ortho-Corrected - 2009 Digital Orthophotos - Washington County

Data.gov (United States)

NSGIC Education | GIS Inventory — This dataset is a collection of GeoTIFF and MrSID format natural color orthophotos covering Washington and Holmes County, Florida. An orthophoto is remotely sensed...

Home energy rating system business plan feasibility study in Washington state

Energy Technology Data Exchange (ETDEWEB)

Lineham, T.

1995-03-01

In the Fall of 1993, the Washington State Energy Office funded the Washington Home Energy Rating System project to investigate the benefits of a Washington state HERS. WSEO established a HERS and EEM Advisory Group. Composed of mortgage lenders/brokers, realtors, builders, utility staff, remodelers, and other state agency representatives, the Advisory Group met for the first time on November 17, 1993. The Advisory Group established several subcommittees to identify issues and options. During its March 1994 meeting, the Advisory Group formed a consensus directing WSEO to develop a HERS business plan for consideration. The Advisory Group also established a business plan subcommittee to help draft the plan. Under the guidance of the business plan subcommittee, WSEO conducted research on how customers value energy efficiency in the housing market. This plan represents WSEO`s effort to comply with the Advisory Group`s request. Why is a HERS Business Plan necessary? Strictly speaking this plan is more of a feasibility plan than a business plan since it is designed to help determine the feasibility of a new business venture: a statewide home energy rating system. To make this determination decision makers or possible investors require strategic information about the proposed enterprise. Ideally, the plan should anticipate the significant questions parties may want to know. Among other things, this document should establish decision points for action.

Promoting accountability: hospital charity care in California, Washington state, and Texas.

Science.gov (United States)

Sutton, Janet P; Stensland, Jeffrey

2004-05-01

Debate as to whether private hospitals meet their charitable obligations is heated. This study examines how alternative state approaches for ensuring hospital accountability to the community affects charitable expenditures and potentially affects access to care for the uninsured. Descriptive and multivariate analyses were used to compare private California hospitals' charity care expenditures with those of hospitals in Texas and Washington state. The key finding from this study is that net of hospital characteristics, market characteristics and community need, Texas hospitals were estimated to provide over 3 times more charity care and Washington hospitals were estimated to provide 66% more charity care than California hospitals. This finding suggests that more prescriptive community benefit or charity care requirements may be necessary to ensure that private hospitals assume a larger role in the care of the uninsured.

Colémbolos (Hexapoda como bioindicadores de la calidad de suelos contaminados con hidrocarburos en el sureste de México Collembola (Hexapoda as quality bioindicators of the hydrocarburans polluted soils in Southestern Mexico

Directory of Open Access Journals (Sweden)

Raúl Uribe-Hernández

2010-04-01

Full Text Available Se evaluaron invertebrados del suelo, en particular los colémbolos, como bioindicadores de la calidad de suelos contaminados con hidrocarburos en el sureste de México. Se realizaron 2 muestreos en verano-otoño del 2004, en 4 parcelas de 2 hectáreas, denominadas zona 1, 2, 3 y control. De cada unidad se tomaron 8 muestras que fueron procesadas por medio del embudo de Berlese-Tullgren y 4 por el método de flotación. Para colémbolos se determinaron los siguientes índices ecológicos: abundancia, riqueza, índice de Shannon (H', dominancia (λ, equidad(J' e índice de similitud (S. Se realizaron análisis fisicoquímicos del suelo: hidrocarburos totales del petróleo (HTP e hidrocarburos aromáticos policíclicos (HAP, porosidad, pH, CE, MO, N, P, K, CIC y textura. Los HTP, en las zonas contaminadas, sobrepasan los límites de las normas mexicanas ambientales. En todas las zonas de estudio se observaron colémbolos, ácaros y larvas de dípteros, por lo que su abundancia y diversidad pueden ser utilizadas como bioindicadores del grado de contaminación y calidad del suelo. En las zonas contaminadas se registraron abundancias muy bajas de Crustacea, Formicidae, Araneae, Diptera, Pseudoscorpionida, y Diplopoda. Las familias de los colémbolos más ampliamente distribuidas fueron Sminthurididae e Isotomidae. De acuerdo con el análisis de correlación, su diversidad de colémbolos es afectada por la presencia de HAP (flouranteno, naftaleno, pireno, criseno y fenantrenoWe evaluated invertebrates, with an emphasis on Collembola, as bioindicators of soil quality in hydrocarbon-contaminated soils in southeastern Mexico. We carried out 2 sampling periods in summer-fall of 2004 in 4 parcels of 2 hectares each. From each parcel we processed 8 samples using the Berlese-Tullgren funnel technique and 4 using the flotation method. For Collembola we calculated the following ecological indices: abundance, richness, the Shannon index (H', dominance (�

O recrutamento militar na América Portuguesa: o esforço conjunto para a defesa da Colônia do Sacramento (1735-1737

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Paulo César Possamai

2004-12-01

Full Text Available In their effort to defend the Colônia de Sacramento, besieged by the Spanish from October 1735 to September 1737, the Portuguese Crown ordered the mobilization of all human and material resources available in the State of Brazil. This article examines the compulsory recruitment that occurred on this occasion and discusses the living conditions of those men who were taken by force from their homes to be sent to the River Platte area, from where few would return.

Avaliação de substratos para reprodução de colêmbolos nativos em condições de laboratório.

Directory of Open Access Journals (Sweden)

Ricardo Bemfica Steffen

2010-08-01

Full Text Available Estudos utilizando a fauna edáfica como bioindicador da qualidade do solo têm sido de grande importância para entender as variações físicas, químicas e biológicas de ecossistemas. Dentre os organismos estudados, os colêmbolos constituem um dos principais grupos. Este trabalho teve como objetivo avaliar a multiplicação de colêmbolos nativos em condições de laboratório. Como substrato, utilizaram-se amostras de solo coletadas em área de citrus e bosque de pinus, com os tratamentos: a amostra de solo de área de cítrus com palha de ervilhaca moída, palha de milho moída, palha de nabo moída, cama de aviário moída e amostra de solo e, b em solo de bosque de pinus com: palha de ervilhaca moída, palha de milho moída, palha de nabo moída, cama de aviário moída e amostra de solo. Os tratamentos foram mantidos em potes plásticos, a 26OC, no escuro, sendo realizadas cinco avaliações durante o período de 180 dias. O solo de pomar de cítrus com adição de palha de nabo moído favoreceu a reprodução destes organismos em condições de laboratório.

Congress in Washington

International Nuclear Information System (INIS)

Anon.

1993-01-01

Full text: Over 1200 accelerator physicists and engineers gathered in Washington mid-May for the 15th in the series of biennial Particle Accelerator Conferences (PAC) - the major US forum for accelerator physics and technology. For the first time since their inception, actual attendance declined, however the number of contributed papers stayed around 1500. CERN Director General designate Chris Llewellyn Smith spelled out the challenges with an opening talk underlining the deficiencies in today's Standard Model. From many directions comes the message that collision in the 1 TeV range must tell us something new - wherefore art thou SSC and LHC? The secondary shock waves of last year's (fortunately overturned) bid to cancel the SSC Superconducting Supercollider project still ripple around the USA, while progress towards authorization of CERN's LHC Large Hadron Collider has been slower than initially hoped. The new US administration has indicated a constant rate of SSC funding over the next four years; the figure is higher than the present budget but considerably below the originally proposed budget profile, implying that completion will be retarded by some three years beyond the end of the decade. The SSC Laboratory will clearly have problems to fight increased overall cost and sustain enthusiasm. CERN hopes for LHC blessing in time to allow machine completion by the year 2000. Pride of place at Washington went to DESY's HERA electron-proton collider - the major new facility since the previous PAC. Commissioning has been impressive and physics is well underway, with luminosity climbing towards the design figure. The varied user community of the ubiquitous synchrotron radiation facilities is now considerably larger than that of particle physics and has extensive industrial involvement. Three such machines have come into operation since the previous PAC - the 6 GeV European Synchrotron Radiation Facility at Grenoble, the 1.5 GeV Advanced Light Source

Termorregulação em colônias de Melipona eburnea (Apidae: Meliponina criadas racionalmente em Rio Branco, Acre

Directory of Open Access Journals (Sweden)

Francisco Cildomar da Silva Correia

2017-08-01

Full Text Available Resumo. A temperatura é o agente climático que afeta mais diretamente o metabolismo das abelhas, influenciando no desenvolvimento das crias, podendo acarretar resultados negativos para produção de mel. O objetivo deste estudo foi conhecer a diferença da termorregulação de colônias de Melipona eburnea Friese. As observações foram realizadas en­tre 09 de junho e 09 de julho de 2016, 24 horas por dia, utilizando-se dois ninhos de M. eburnea, um em caixa racional e outro em troncode árvore. Utilizou-se Data Logger(modelo HOBO U12 – 012, com exatidão de ±0,35 °C. Verificou-se que o ninho de M. eburnea em oco de árvore manteve uma temperatura média de 31,7 ºC, enquanto que o ninho em caixa racional apresentou temperatura média de 27,8 ºC. A temperatura ambiente variou de 16,6 a 34,2 °C, com média de 23,8 ºC. A espécie M. eburnea em caixa racional apresentou termorregulação inferior à colônia em oco de árvore e também maior oscilação de temperatura. Concluiu-se que M. eburnea mantém sua colônia em homeostase, mesmo quando há variações na temperatura ambiente (ninho em oco de árvore. M. eburnea apresentou termorregulação insatisfatória, quando mantida em caixa racional. Thermoregulation in colonies of Melipona eburnea Friese(Apidae: Meliponina rationally bred in Rio Branco, Acre Abstract. Temperature is the climatic agent that most directly affects the metabolism of bees, influencing at the development of the young being able lead to negative results for honey production. The objective of this study was to know the difference of thermoregulation of Melipona eburnea Friese colonies. The observations were made between June 9 and July 9, 2016, 24 hours a day, using two nests of M. eburnea, one in rational box and the other in tree trunk. Was used Data Logger (model HOBO U12-012 was used, with accuracy of ± 0.35 °C. Was verified that the nest of M. eburnea in tree hollow maintained an average temperature of 31

The Relationships between the Iowa Test of Basic Skills and the Washington Assessment of Student Learning in the State of Washington. Technical Report.

Science.gov (United States)

Joireman, Jeff; Abbott, Martin L.

This report examines the overlap between student test results on the Iowa Test of Basic Skills (ITBS) and the Washington Assessment of Student Learning (WASL). The two tests were compared and contrasted in terms of content and measurement philosophy, and analyses studied the statistical relationship between the ITBS and the WASL. The ITBS assesses…

Large-scale silviculture experiments of western Oregon and Washington.

Science.gov (United States)

Nathan J. Poage; Paul D. Anderson

2007-01-01

We review 12 large-scale silviculture experiments (LSSEs) in western Washington and Oregon with which the Pacific Northwest Research Station of the USDA Forest Service is substantially involved. We compiled and arrayed information about the LSSEs as a series of matrices in a relational database, which is included on the compact disc published with this report and...

Unique mutation portraits and frequent COL2A1 gene alteration in chondrosarcoma

Science.gov (United States)

Totoki, Yasushi; Yoshida, Akihiko; Hosoda, Fumie; Nakamura, Hiromi; Hama, Natsuko; Ogura, Koichi; Yoshida, Aki; Fujiwara, Tomohiro; Arai, Yasuhito; Toguchida, Junya; Tsuda, Hitoshi; Miyano, Satoru; Kawai, Akira

2014-01-01

Chondrosarcoma is the second most frequent malignant bone tumor. However, the etiological background of chondrosarcomagenesis remains largely unknown, along with details on molecular alterations and potential therapeutic targets. Massively parallel paired-end sequencing of whole genomes of 10 primary chondrosarcomas revealed that the process of accumulation of somatic mutations is homogeneous irrespective of the pathological subtype or the presence of IDH1 mutations, is unique among a range of cancer types, and shares significant commonalities with that of prostate cancer. Clusters of structural alterations localized within a single chromosome were observed in four cases. Combined with targeted resequencing of additional cartilaginous tumor cohorts, we identified somatic alterations of the COL2A1 gene, which encodes an essential extracellular matrix protein in chondroskeletal development, in 19.3% of chondrosarcoma and 31.7% of enchondroma cases. Epigenetic regulators (IDH1 and YEATS2) and an activin/BMP signal component (ACVR2A) were recurrently altered. Furthermore, a novel FN1-ACVR2A fusion transcript was observed in both chondrosarcoma and osteochondromatosis cases. With the characteristic accumulative process of somatic changes as a background, molecular defects in chondrogenesis and aberrant epigenetic control are primarily causative of both benign and malignant cartilaginous tumors. PMID:25024164

Energy Savings Analysis of the Proposed Revision of the Washington D.C. Non-Residential Energy Code

Energy Technology Data Exchange (ETDEWEB)

Rosenberg, Michael I.; Athalye, Rahul A.; Hart, Philip R.

2017-12-01

This report presents the results of an assessment of savings for the proposed Washington D.C. energy code relative to ASHRAE Standard 90.1-2010. It includes annual and life cycle savings for site energy, source energy, energy cost, and carbon dioxide emissions that would result from adoption and enforcement of the proposed code for newly constructed buildings in Washington D.C. over a five year period.

Ergonomics and regulatory politics: the Washington State case.

Science.gov (United States)

Silverstein, Michael

2007-05-01

Every year in the State of Washington more than 50,000 workers experience a work related musculoskeletal disorder (WMSD), making up more than 30% of all worker compensation cases. In 2000, the Washington State Department of Labor and Industries (L&I) adopted a workplace ergonomics rule requiring employers to reduce worker exposure to hazards that cause or contribute to WMSDs. In 2003, the ergonomics rule was repealed by a margin of 53.5-46.5 in a statewide voter initiative. The official rulemaking record of approximately 100,000 pages, along with supplementary published and unpublished material, was reviewed. The relationship between scientific deliberation and the public policy process in adopting and repealing the ergonomics rule was assessed and described. The deliberative features of the regulatory, judicial, legislative, and ballot processes were compared. The ergonomics rule was successful in the regulatory and legal arenas where the process was most transparent and open to public involvement, differing views could be presented fully, and decision makers were expected to explain their decisions in light of the record. The rule fared most poorly in the legislature and at the ballot box when these features were lost and where considered deliberation was replaced by unconstrained political conflict. Additional checks and balances are needed.

Washington International Renewable Energy Conference 2008 Pledges: Methodology and Assumptions Summary

Energy Technology Data Exchange (ETDEWEB)

Babiuch, B.; Bilello, D. E.; Cowlin, S. C.; Mann, M.; Wise, A.

2008-08-01

The 2008 Washington International Renewable Energy Conference (WIREC) was held in Washington, D.C., from March 4-6, 2008, and involved nearly 9,000 people from 125 countries. The event brought together worldwide leaders in renewable energy (RE) from governments, international organizations, nongovernmental organizations, and the private sector to discuss the role that renewables can play in alleviating poverty, growing economies, and passing on a healthy planet to future generations. The conference concluded with more than 140 governments, international organizations, and private-sector representatives pledging to advance the uptake of renewable energy. The U.S. government authorized the National Renewable Energy Laboratory (NREL) to estimate the carbon dioxide (CO2) savings that would result from the pledges made at the 2008 conference. This report describes the methodology and assumptions used by NREL in quantifying the potential CO2 reductions derived from those pledges.

78 FR 46258 - Drawbridge Operation Regulation Lake Washington, Seattle, WA

Science.gov (United States)

2013-07-31

... that governs the Evergreen Point Floating Bridge (State Route 520 across Lake Washington) at Seattle... Department of Transportation has requested that the draw span of the Evergreen Point Floating Bridge (State... this time, which would divert road traffic onto the Evergreen Point Floating Bridge. The closure of the...

Family/Educator Guide to Washington's Special Education Services. Revised Edition.

Science.gov (United States)

Molloy, Patty

This guide, designed for families of children with disabilities and educators in Washington state, is intended to provide a shared understanding of special education and encourage partnership for the child's benefit. Each section specifies what families and educators should expect, their responsibilities, possible questions to ask, and their…

Radiochemistry Education at Washington State University: Sustaining Academic Radiochemistry for the Nation

International Nuclear Information System (INIS)

Clark, Sue B.; Nash, Ken; Benny, Paul; Clark, Aurora; Wall, Nathalie; Wall, Don; Yoo, Choong-Shik

2009-01-01

Since 2002, Washington State University has been building radiochemistry as a component of its overall chemistry program. Using an aggressive hiring strategy and leveraged funds from the state of Washington and federal agencies, six radiochemistry faculty members have been added to give a total of seven radiochemists out of a department of twenty-five faculty members. These faculty members contribute to a diverse curriculum in radiochemistry, and the Chemistry Department now enjoys a significant increase in the number of trainees, the quantity of research expenditures, and the volume and quality of peer-reviewed scientific literature generated by the radiochemistry faculty and the trainees. These three factors are essential for sustaining the radiochemistry education and research program at any academic institution.

Prevalence of corporal punishment among students in Washington State schools.

Science.gov (United States)

Grossman, D C; Rauh, M J; Rivara, F P

1995-05-01

To determine the prevalence of corporal punishment in Washington State and the factors associated with its use in Washington elementary and secondary schools. Cross-sectional mail survey performed during the summer of 1992. All elementary and secondary schools in the state of Washington. One thousand eighteen schools (47%) responded to the survey, of which 80% were publicly funded and 63% were located in urban areas. The study sample closely resembled the profile of all schools in the state. Almost 11% of participating schools permitted corporal punishment at the time of the survey and 3.2% reported its actual use during the 1991-1992 school year, resulting in an estimated prevalence of 7.2 incidents per 1000 students per year. Sixteen percent of corporal punishment actions occurred in schools not permitting its use. Ninety percent of public schools relied on district policy regarding corporal punishment. School characteristics associated with the use of corporal punishment included rural location (crude odds ratio, 2.2; 95% confidence interval, 1.5 to 3.4), enrollment of less than 500 students (crude odds ratio, 1.7; 95% confidence interval, 1.1 to 2.7), and kindergarten to eighth-grade or kindergarten to 12th-grade enrollment (crude odds ratio, 2.5; 95% confidence interval, 1.6 to 3.9). The lack of a statewide ban on school corporal punishment at the time of this survey was associated with the continued use of corporal punishment against children in districts that continued to permit it. School policies against corporal punishment were associated with much lower prevalence. Continued efforts are needed to enact and enforce laws in the remaining states that have not yet banned corporal punishment.

Geologic map of the Washington West 30’ × 60’ quadrangle, Maryland, Virginia, and Washington D.C.

Science.gov (United States)

Lyttle, Peter T.; Aleinikoff, John N.; Burton, William C.; Crider, E. Allen; Drake, Avery A.; Froelich, Albert J.; Horton, J. Wright; Kasselas, Gregorios; Mixon, Robert B.; McCartan, Lucy; Nelson, Arthur E.; Newell, Wayne L.; Pavlides, Louis; Powars, David S.; Southworth, C. Scott; Weems, Robert E.

2018-01-02

The Washington West 30’ × 60’ quadrangle covers an area of approximately 4,884 square kilometers (1,343 square miles) in and west of the Washington, D.C., metropolitan area. The eastern part of the area is highly urbanized, and more rural areas to the west are rapidly being developed. The area lies entirely within the Chesapeake Bay drainage basin and mostly within the Potomac River watershed. It contains part of the Nation's main north-south transportation corridor east of the Blue Ridge Mountains, consisting of Interstate Highway 95, U.S. Highway 1, and railroads, as well as parts of the Capital Beltway and Interstate Highway 66. Extensive Federal land holdings in addition to those in Washington, D.C., include the Marine Corps Development and Education Command at Quantico, Fort Belvoir, Vint Hill Farms Station, the Naval Ordnance Station at Indian Head, the Chesapeake and Ohio Canal National Historic Park, Great Falls Park, and Manassas National Battlefield Park. The quadrangle contains most of Washington, D.C.; part or all of Arlington, Culpeper, Fairfax, Fauquier, Loudoun, Prince William, Rappahannock, and Stafford Counties in northern Virginia; and parts of Charles, Montgomery, and Prince Georges Counties in Maryland.The Washington West quadrangle spans four geologic provinces. From west to east these provinces are the Blue Ridge province, the early Mesozoic Culpeper basin, the Piedmont province, and the Coastal Plain province. There is some overlap in ages of rocks in the Blue Ridge and Piedmont provinces. The Blue Ridge province, which occupies the western part of the quadrangle, contains metamorphic and igneous rocks of Mesoproterozoic to Early Cambrian age. Mesoproterozoic (Grenville-age) rocks are mostly granitic gneisses, although older metaigneous rocks are found as xenoliths. Small areas of Neoproterozoic metasedimentary rocks nonconformably overlie Mesoproterozoic rocks. Neoproterozoic granitic rocks of the Robertson River Igneous Suite intruded

Produção de geleia real em colônias de abelhas africanizadas considerando diferentes suplementos proteicos e a influência de fatores ambientais = Royal jelly production in Africanized honeybee colonies considering different protein supplements and the influence of environmental factors

Directory of Open Access Journals (Sweden)

Vagner de Alencar Arnaut de Toledo

2010-01-01

Full Text Available O objetivo foi avaliar o efeito da suplementação proteica sobre a produção de geleia real em colônias de Apis mellifera e correlacionar esta produção com as variáveis climáticas. Os tratamentos foram: colônias que receberam uma ração elaborada com 35% de proteína; colônias que receberam suplemento proteico comercial com 35% de proteína bruta e colônias que não receberam ração proteica. Não houve diferença (p > 0,05 entre os tratamentos para as variáveis de produção. As médias foram: quantidade de larvas aceitas, 8,56 ± 6,76; porcentagem de aceitação, 29,20 ± 22,03%; quantidade de geleia real produzida por colônia, 1,83 ± 0,82 g e quantidade produzida por cúpula, 213,58 ± 108,36 mg. A precipitação pluviométrica não influenciou (p > 0,05 a produção de geleia real. Os modelos selecionados evidenciaram que houve efeito negativo da temperatura máxima e umidade relativa mínima do ar com número de larvas aceitas. Observou-se que a porcentagem de aceitação de larvas e a produção de geleia real por colônias apresentaram correlação positiva com a umidade relativa máxima e negativa com a umidade relativa mínima. Pode-se concluir que a adição de suplemento proteico (35% nãoaumentou a produção de geleia real das colônias de abelhas africanizadas e seu uso para esta finalidade foi economicamente inviável.This research was carried out to evaluate the effect of proteic supplement on royal jelly production in honeybee colonies and its correlation with environmental variables. The treatments were: colonies that had received a diet formulated with 35% crude protein, colonies that had received a commercial supplement with 35% crude protein, and colonies that did not receive protein diet. There was nodifference (p > 0.05 among the treatments for parameters of production. The average for each variable was: amount accepted larvae, 8.56 ± 6.76; acceptance percentage, 29.20 ± 22.03%; amount of royal jelly

Recent developments: Washington focus

International Nuclear Information System (INIS)

Anon.

1990-01-01

Congress reconvened on January 23, but most of Washington's January new involves the Administration. DOE sent two letters to USEC customers, awarded a contract for the independent financial review of the enrichment program, and released a plan for demonstrating AVLIS by 1992. A General Accounting Office (GAO) report investigating the impact of imports of Soviet EUP into the US was made public. Both Congress and the administration are reportedly considering a full-scope US-Soviet Agreement for Nuclear Cooperation. Finally, published reports indicate Congress may consider ending the customs user fee which levies a charge of 0.17% on the value of all imported goods. The fee is felt to violate the General Agreement on Tariffs and Trade (GATT) and is not based on recovering actual Customs costs for processing a good. The fee brings the Treasury over $700 million per year, but the business community plans to lobby hard for its outright elimination or a change in authority to collect the fee based on actual costs

Water resources of King County, Washington

Science.gov (United States)

Richardson, Donald; Bingham, J.W.; Madison, R.J.; Williams, R.

1968-01-01

Although the total supply of water in King County is large, water problems are inevitable because of the large and rapidly expanding population. The county contains a third of the 3 million people in Washington, most of the population being concentrated in the Seattle metropolitan area. King County includes parts of two major physiographic features: the western area is part of the Puget Sound Lowland, and the eastern area is part of the Cascade Range. In these two areas, the terrain, weather, and natural resources (including water) contrast markedly. Average annual precipitation in the county is about 80 inches, ranging from about 30 inches near Puget Sound to more than 150 inches in parts of the Cascades. Annual evapotranspiration is estimated to range from 15 to 24 inches. Average annual runoff ranges from about 15 inches in the lowlands to more than 100 inches in the mountains. Most of the streamflow is in the major basins of the county--the Green-Duwamish, Lake Washington, and Snoqualmie basins. The largest of these is the Snoqualmie River basin (693 square miles), where average annual runoff during the period 1931-60 was about 79 inches. During the same period, annual runoff in the Lake Washington basin ( 607 square miles) averaged about 32 inches, and in the Green-Duwamish River basin (483 square miles), about 46 inches. Seasonal runoff is generally characterized by several high-flow periods in the winter, medium flows in the spring, and sustained low flows in the summer and fall. When floods occur in the county they come almost exclusively between October and March. The threat of flood damage is greatest on the flood plaits of the larger rivers, but in the Green-Duwamish Valley the threat was greatly reduced with the completion of Howard A. Hanson Dam in 1962. In the Snoqualmie River basin, where no such dam exists, the potential damage from a major flood increases each year as additional land is developed in the Snoqualmie Valley. 0nly moderate amounts of

ABNORMAL TYPE-III COLLAGEN PRODUCED BY AN EXON-17-SKIPPING MUTATION OF THE COL3A1 GENE IN EHLERS-DANLOS SYNDROME TYPE-IV IS NOT INCORPORATED INTO THE EXTRACELLULAR-MATRIX

NARCIS (Netherlands)

CHIODO, AA; SILLENCE, DO; COLE, WG; BATEMAN, JF

1995-01-01

A novel heterozygous mutation of the COL3Al gene that encodes the alpha 1(III) chains of type III collagen was identified in a family with the: acrogeric form of Ehlers-Danlos syndrome type IV (EDS-IV). Cultured dermal fibroblasts produced normal and shortened alpha 1(III) chains. The triple helix

Efeito do colágeno na maciez da carne de bovinos de distintos grupos genéticos = Collagen effects in meat tenderness of bovines of different genetic groups

Directory of Open Access Journals (Sweden)

Daniela Cristina Morales

2006-01-01

Full Text Available O objetivo do trabalho foi avaliar a influência do colágeno na maciez da carne de animais de diferentes grupos genéticos produzidos no sistema de produção do novilho superprecoce. Foram utilizados bezerros machos inteiros da raça Nelore, mestiços ½ Nelore x ½ Aberdeen Angus e mestiços ½ Nelore x ½ Simental. Após abate e resfriamento por 24 horas, foram retiradas amostras do músculo Longissimus dorsi, na região entre a 11a e a 13a costela, sendo que uma amostra foi congelada e as demais maturadas por 7 e 14 dias. Nãohouve diferença significativa (P>0,01 entre os grupos genéticos para a quantidade e a solubilidade de colágeno e a força de cisalhamento. A quantidade e a solubilidade do colágeno não comprometeram a maciez da carne, indiferentemente do grupo genético utilizado e do tempo postmortem, tornando vantajosa a opção de se abaterem animais jovens.The aim of work was to analyze the collagen effect in meat tenderness of animals of different genetic groups produced by very young bullock production system. Male calves Nellore purebred, ½ Nellore x ½ Aberdeen Angus and ½ Nellore x ½ Simmental crossbred were used.After slaughter and cooling for 24 hours Longissimus dorsi samples were removed, between 11th and 13th ribs, one sample was frozen and the others ageing for 7 and 14 days. There was no difference (P>0,01 between genetic groups for amount and heat soluble collagen andshear force values. The amount and solubility collagen don’t compromised the meat tenderness, indifferent of the genetic group used and postmortem period, becoming the early slaughter an advantageous option for meat production with desirable characteristics.

Sidwell Friends Quaker School, Washington, DC, by KieranTimberlake Architects

DEFF Research Database (Denmark)

Vibæk, Kasper Sánchez; Stylsvig Madsen, Ulrik

2012-01-01

In 2006 the new Middle School Building on the campus of Sidwell Friends School in Washington DC was completed at a cost of $28 million. The project is a renovation and addition to a fifty-year-old educational facility transforming the building and the surrounding landscape into a green expression...

O estabelecimento de colônias agrícolas civis e militares na província do Piauí no pós-guerra do Paraguai (1865-1888 = The establishment of military and civil agricultural colonies in the province of Piauí in after Paraguay war period (1865-1888

Directory of Open Access Journals (Sweden)

Araújo, Johny Santana de

2013-01-01

Full Text Available O presente artigo pretende analisar a ação do governo imperial e provincial a fim de promover a ocupação do território do sul e sudoeste do Piauí através da criação de colônias civis e militares, processo amplamente desenvolvido entre os anos de 1870 a 1889 cujo um dos objetivos era assentar os ex-combatentes que retornavam da guerra do Paraguai e cujas promessas contidas no decreto 3. 371 de 1865 previam a distribuição de terras em colônias agrícolas civis e militares. Outra questão de relevância na proposta do estudo esta relacionada ao objetivo do governo imperial e provincial em reavivar os antigos projetos de criação de colônias agrícolas, amplamente defendidas pelo General Couto Magalhães e por Francisco Adolfo de Varnhagen

Washington State biomass data book

International Nuclear Information System (INIS)

Deshaye, J.A.; Kerstetter, J.D.

1991-07-01

This is the first edition of the Washington State Biomass Databook. It assess sources and approximate costs of biomass fuels, presents a view of current users, identifies potential users in the public and private sectors, and lists prices of competing energy resources. The summary describes key from data from the categories listed above. Part 1, Biomass Supply, presents data increasing levels of detail on agricultural residues, biogas, municipal solid waste, and wood waste. Part 2, Current Industrial and Commercial Use, demonstrates how biomass is successfully being used in existing facilities as an alternative fuel source. Part 3, Potential Demand, describes potential energy-intensive public and private sector facilities. Part 4, Prices of Competing Energy Resources, shows current suppliers of electricity and natural gas and compares utility company rates. 49 refs., 43 figs., 72 tabs

Empowerment Zones and Enterprise Districts, Enterprise Zones: Boundaries of Maryland's Enterprise Districts that are within Washington County., Published in 2006, 1:7200 (1in=600ft) scale, Washington County Government.

Data.gov (United States)

NSGIC Local Govt | GIS Inventory — Empowerment Zones and Enterprise Districts dataset current as of 2006. Enterprise Zones: Boundaries of Maryland's Enterprise Districts that are within Washington...

Relatório sobre o colóquio Brasil-Japão: 25-27 de julho de 1966

Directory of Open Access Journals (Sweden)

Eurípedes Simões de Paula

1966-09-01

Full Text Available São Paulo, o mais expressivo núcleo urbano de influência japonesa em tôda a América Latina, abrigou, de 25 a 27 de julho de 1966, o I Colóquio Brasil-Japão. Nessa oportunidade encontraram-se professôres japonêses e brasileiros que juntos debateram temas culturais no campo de suas especialidades, cuja fecundidade pôde ser verificada pela vivacidade dos debates travados e pelo grande número de interessados que compareceram a tôdas as sessões realizadas no Departamento de História da Faculdade de Filosofia, Ciências e Letras da Universidade de São Paulo, situado na Cidade Universitária "Armando de Sales Oliveira, no Butantã, onde também funciona a Secção de Estudos Orientais da mesma Universidade.

Bushy-tailed woodrat abundance in dry forests of eastern Washington.

Science.gov (United States)

John F. Lehmkuhl; Keith D. Kistler; James S. Begley

2006-01-01

We studied bushy-tailed woodrats (Neotonza cinerea occidentalis) in the eastern Washington Cascade Range to estimate their density and survival in 3 typical dry forest cover types. We predicted woodrat density to be high, moderate, and low in mature mixed-conifer forests, young mixed-conifer forests, and open ponderosa pine forests, respectively....

Productivity of nonindustrial private forests in western Washington: alternative futures.

Science.gov (United States)

Ralph J. Alig; Darius M. Adams

1995-01-01

Nonindustrial private timberlands in western Washington have high productive potential and contribute harvest amounts somewhat more than proportional to their area. Of all private ownerships they are influenced the most by land use shifts and are affected in important ways by forest practice regulations. About 1 million acres of nonindustrial private timberland contain...

Custeio Baseado em Atividades (ABC Aplicado ao Setor Público: Estudo de Caso no Colégio Militar do Rio de Janeiro (CMRJ

Directory of Open Access Journals (Sweden)

Luis Paulo Faria Carmo, Lino Martins Silva

2011-04-01

Full Text Available O objetivo desta pesquisa foi verificar se o método de Custeio Baseado em Atividades (ABC é adequado para ser aplicado na mensuração dos custos educacionais no Colégio Militar do Rio de Janeiro. Embora a Lei federal nº. 4.320/64 já determinasse a necessidade de apuração dos custos, ingressos e resultados, a Administração Pública ainda não sabe quanto custa cada uma de suas atividades. A relevância do tema deriva da necessidade de as Organizações Públicas de permitir o acompanhamento e a avaliação de sua gestão. Porém, o Sistema Integrado de Administração Financeira do Governo Federal (SIAFI, principal ferramenta do governo, não evidencia em suas contas o custo de cada atividade. A metodologia utilizada teve como “principal finalidade desenvolver, esclarecer e modificar idéias a fim de fornecer hipótese pesquisável para estudos posteriores” (Tripodi, Fellin e Meyer, 1981, p.64 e como fontes de pesquisa a revisão bibliográfica e a análise documental, sendo utilizado como estratégia metodológica de um estudo de caso real no Colégio Militar do Rio de Janeiro (CMRJ. A análise dos resultados aponta ser adequada a aplicação do método de Custeio Baseado em Atividades (ABC para evidenciação dos custos das atividades educacionais, bem como subsidiar a avaliação da qualidade do gasto Público.

Organics Verification Study for Sinclair and Dyes Inlets, Washington

Energy Technology Data Exchange (ETDEWEB)

Kohn, Nancy P.; Brandenberger, Jill M.; Niewolny, Laurie A.; Johnston, Robert K.

2006-09-28

Sinclair and Dyes Inlets near Bremerton, Washington, are on the State of Washington 1998 303(d) list of impaired waters because of fecal coliform contamination in marine water, metals in sediment and fish tissue, and organics in sediment and fish tissue. Because significant cleanup and source control activities have been conducted in the inlets since the data supporting the 1998 303(d) listings were collected, two verification studies were performed to address the 303(d) segments that were listed for metal and organic contaminants in marine sediment. The Metals Verification Study (MVS) was conducted in 2003; the final report, Metals Verification Study for Sinclair and Dyes Inlets, Washington, was published in March 2004 (Kohn et al. 2004). This report describes the Organics Verification Study that was conducted in 2005. The study approach was similar to the MVS in that many surface sediment samples were screened for the major classes of organic contaminants, and then the screening results and other available data were used to select a subset of samples for quantitative chemical analysis. Because the MVS was designed to obtain representative data on concentrations of contaminants in surface sediment throughout Sinclair Inlet, Dyes Inlet, Port Orchard Passage, and Rich Passage, aliquots of the 160 MVS sediment samples were used in the analysis for the Organics Verification Study. However, unlike metals screening methods, organics screening methods are not specific to individual organic compounds, and are not available for some target organics. Therefore, only the quantitative analytical results were used in the organics verification evaluation. The results of the Organics Verification Study showed that sediment quality outside of Sinclair Inlet is unlikely to be impaired because of organic contaminants. Similar to the results for metals, in Sinclair Inlet, the distribution of residual organic contaminants is generally limited to nearshore areas already within the

Mineralogia, inclusões fluídas e gênese de esmeraldas das jazidas de Chivor, Coscuez, Muzo, Pacho e Yacopi, Colômbia

OpenAIRE

Fernando Heli Romero Ordóñez

1993-01-01

As jazidas de esmeralda da Colômbia estão localizadas na parte central da Cordilheira Oriental, nas regiões do Guavio e do Território Vásquez-Yacopí. Estas mineralizações de idade terciária, estão localizadas em rochas sedimentares marinhas de idade cretácea inferior, pertencentes às unidades estratigráficas Calizas del Guavio e Lutitas de Macanal, ambas situadas na região do Guavio, e às unidades Rosa Blanca e Paja localizadas no Território Vásquez-Yacopí. As características de posicionament...

Managing the Development of the Public Telecommunications Center, Spokane, Washington.

Science.gov (United States)

Schaar, Walter

When the city council of Spokane (Washington) decided in 1971 to establish a cable franchise, it created a citizens' committee to set cable specifications. Representing Spokane School District 81 and KSPS-TV (a public television station licensed to the public schools of Spokane), the author of this document served on the committee that set five…

The State of Washington's Children, Fall 2001. [Ninth Annual Report].

Science.gov (United States)

Washington Univ., Seattle. School of Public Health and Community Medicine.

This Kids Count report is the ninth to examine annually statewide trends in the well-being of Washington's children. The statistical portrait is based on indicators of child well-being in five areas: (1) family and community, including teen birth rate, teen pregnancy rate, divorces involving children, and births to unmarried mothers; (2) economic…

Escolas traçadas com régua e compasso na Colômbia e no Brasil

Directory of Open Access Journals (Sweden)

Carlos Jilmar Diaz Soler

Full Text Available Com este artigo, que é parte de uma pesquisa mais ampla, pretendemos refletir sobre o processo de escolarização da infância, em duas revistas destinadas à formação de professores para o magistério. Ambas produzidas por grupos articulados a políticas educacionais e divulgadas para as redes públicas de ensino de São Paulo (Brasil e outra para Bogotá (Colômbia, de 1932 a 1939. Fizemos duas perguntas às revistas: O que podem nos dizer sobre as finalidades políticas da formação de professores para o magistério? E sobre as relações entre os métodos científicos da psicologia e da pedagogia? No final, apresentamos nossas suspeitas de cumplicidade entre projetos pedagógicos e projetos políticos autoritários, atuando no conjunto das forças cognitivas, psicológicas, culturais e institucionais.

Report of the Annual Scientific Sessions of the American College of Cardiology (ACC), Washington DC.

Science.gov (United States)

Hiro, Takafumi

2017-05-25

The 66 th Annual Scientific Sessions and Expo of the American College of Cardiology (ACC) were held at the Walter E. Washington Convention Center, Washington DC, from March 17 th to 19 th , 2017. This meeting offered 23 Late-Breaking Clinical Trial (LBCT) presentations, 17 Featured Clinical Research presentations with and without LBCT, and 2,572 abstracts presented in oral and poster sessions by over 2,000 experts. This report presents the highlights of this meeting, including the opening showcase, several important LBCTs and some international joint symposiums.

Cartografia e deficiência visual: experiências no Colégio Pedro II

Directory of Open Access Journals (Sweden)

Rafael Medeiros de Andrade

2014-01-01

Full Text Available O processo de construção de uma escola inclusiva perpassa um conjunto de esforços cognitivos e técnicos por parte da comunidade escolar que são necessários para a adequada educação e desenvolvimento do aluno. No que diz respeito ao ensino de Geografia para alunos deficientes visuais, fazem parte deste conjunto de medidas o domínio do sistema Braille, a confecção de materiais e mapas táteis, a gravação de textos em áudio e, entre outras, a realização de trabalhos de campo. O presente relato de experiência refere-se à um projeto de dedicação exclusiva, implementado no Colégio Pedro II de 2008 a 2010, cujo objetivo foi aprimorar o ensino de Geografia para os alunos deficientes visuais. Conclui-se que a adoção dessas medidas representa o início de um longo trabalho de toda comunidade escolar que não pode ser resumido à existência de alguns materiais táteis. Deve, por outro lado, envolver um processo de construção e adequação instrumental, acompanhado da capacitação profissional e da sensibilização da comunidade escolar.

Memorial—John A. Washington II, M.D.▿

OpenAIRE

2010-01-01

John A. Washington II, M.D., former Head of Clinical Microbiology at the Mayo Clinic from 1972 to 1986 and Chairman of the Department of Microbiology at the Cleveland Clinic from 1986 to 1992, died on 5 September 2010 at the age of 74. John was an internationally recognized, widely respected leader in the disciplines of clinical microbiology and infectious diseases, authoring more than 450 scientific articles, books, and book chapters and training scores of pathology residents and clinical mi...

Investigando os rumos da disciplina escolar Ciências no Colégio Pedro II: 1960-1970 A research about the history of the General Sciences as a school subject in the Colégio Pedro II: 1960-1970

Directory of Open Access Journals (Sweden)

Marcia Serra Ferreira

2007-06-01

Full Text Available Este artigo analisa mecanismos de estabilidade e mudança curriculares produzidos no Colégio Pedro II que influenciaram os rumos da disciplina escolar Ciências no 2º segmento do Ensino Fundamental, durante os anos 1960-1970. Investigando fontes orais e escritas, afirmo que aspectos institucionais puderam contribuir para que esta sofresse influências do movimento de renovação do ensino de Ciências ocorrido no período. Tais aspectos dizem respeito ao baixo status da disciplina escolar, que possuía um caráter mais generalista, era preterida pelo catedrático e não possuía espaço físico próprio. Essas características viabilizaram mudanças em uma instituição com arraigadas tradições e com as decisões centralizadas nos catedráticos. Tais mudanças, no entanto, articularam as inovações com conteúdos e práticas do passado, inventando uma tradição que pôde agregar o tradicional/antigo com o moderno/renovado e manter o prestígio da disciplina escolar tanto internamente quanto no nível externo.This paper analyses both the stability and the curriculum changing mechanisms produced in the Colégio Pedro II, which have influenced the course of Science as a school subject in upper elementary school during the 1960-1970'. Based on written and oral sources, the researcher states that institutional aspects had contributed to the fact that the influences from the Science Education innovation movement could be processed in the school subject. The institutional aspects were related to the school subject low status due to the fact that it had a general characteristic, it was less preferred by the head of department and it had not a specific physical space for the lessons. Those characteristics allowed room for changes in a long-term traditional institution in which curriculum decisions used to be centralized on the heads of departments themselves. Such curriculum changes had articulated innovations to teaching contents and practices

Description of a new species of the spider genus Syntrechalea (Araneae: Lycosoidea: Trechaleidae from Colombia Descrição de uma nova espécie de Syntrechalea (Araneae: Lycosoidea: Trechaleidae da Colômbia

Directory of Open Access Journals (Sweden)

Estevam L. C. da Silva

2008-09-01

Full Text Available A new species of the spider genus Syntrechalea F.O.P.-Cambridge, 1902 is described and illustrated based on material collected in Loreto Mocagua, Colombia. Representatives of this spider genus exhibit an arboreal foraging behavior and are distinguished from the other genera of Trechaleidae by the flattened carapace, long legs and flexible metatarsi and tarsi. New records on the distribution of Syntrechalea tenuis F.O.P.Cambridge, 1902 in Colombia are presented.Uma espécie nova do gênero Syntrechalea F.O.P.-Cambridge, 1902 é descrita e ilustrada, a partir de material coletado em Loreto Mocagua, Colômbia. Os representantes deste gênero apresentam o hábito arbóreo de forragear e são distinguidos dos demais gêneros de Trechaleidae pela carapaça achatada,pernas longas e metatarsos e tarsos flexíveis. Novos registros de distribuição de Syntrechalea tenuis F.O.P.-Cambridge, 1902 na Colômbia são apresentados.

Caracterização sazonal de acúmulos isolados de própolis em colônias de Plebeia emerina (Hymenoptera, Apidae no sul do Brasil Seasonal characterization of isolated propolis clusters in Plebeia emerina (Hymenoptera, Apidae colonies in the south of Brazil

Directory of Open Access Journals (Sweden)

Camila G. dos Santos

2009-06-01

Full Text Available Em colônias de abelhas sem ferrão a aplicação da própolis é ampla, sendo utilizada como matéria-prima nas construções e defesa contra inimigos. Há registros de armazenamento de própolis viscosa, sob forma de acúmulos isolados. Neste trabalho propõe-se a caracterização sazonal da área, do número e da distribuição espacial dos acúmulos isolados de própolis em colônias de Plebeia emerina (Friese, 1900. Colônias foram avaliadas entre outubro/2003 e setembro/2004, medindo-se mensalmente os acúmulos isolados de própolis e registrando-se a posição relativa dos mesmos nas colméias. Entre outubro e março, a área dos acúmulos de própolis nas colônias variou entre 0,50 e 4,92 cm² e o número de acúmulos foi de 3 a 16. No período de abril a setembro, a área foi de 4,54 a 18,48 cm² e o número de acúmulos de 9 a 36. Sugere-se que o aumento da própolis acumulada possa estar relacionado à preparação das colônias para o outonoinverno quando a coleta do produto é reduzida. A análise sazonal da distribuição dos depósitos isolados de própolis corrobora com os registros da área total, indicando preferência da posição anterior da colônia para acumular a própolis. Esta constatação fortalece a hipótese do uso da própolis viscosa dos depósitos isolados na defesa, principalmente junto à entrada das colônias.In colonies of stingless bees, propolis is used for many applications, such as in raw material for constructions and for their defense against enemies. There are records of viscous propolis storage, in form of isolated clusters. In this work, the seasonal characterization of area, number and spatial distribution of isolated propolis clusters in Plebeia emerina (Friese, 1900 colonies is proposed. Colonies were evaluated between October/2003 and September/2004, by measuring in a monthly basis the isolated propolis clusters and recording the relative position of these clusters within the beehives. Between

A comparison of operational performance : Washington state ferries to ferry operators worldwide.

Science.gov (United States)

2010-06-01

This project compares eight measures of performance related to transit service quality (e.g. trip reliability, on-time departures) and cost-efficiency (e.g. farebox recovery, subsidy per passenger) between Washington State Ferries (WSF) and 23 ferry ...

The State of Washington's Children, Summer 2002. [Tenth Annual Report].

Science.gov (United States)

Connell, Frederick A.; Brandon, Richard; Hill, Sheri L.; Carter, S. Louise; Garrison, Michelle M.; DeWys, Shelley; Mandell, Dorothy J.

This Kids Count report is the tenth to examine annually statewide trends in the well-being of Washington's children and focuses on child poverty and the needs of the working poor. The statistical portrait is based on indicators of child well-being in five areas: (1) family and community, including teen birth rate, teen pregnancy rate, births to…

78 FR 24981 - Irish Potatoes Grown in Washington; Decreased Assessment Rate

Science.gov (United States)

2013-04-29

... Reform. Under the marketing order now in effect, Washington potato handlers are subject to assessments... DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 946 [Doc. No. AMS-FV-13-0010... Marketing Service, USDA. ACTION: Interim rule with request for comments. SUMMARY: This rule decreases the...

Carles Ros i Hebrera. Col·loqui nou, graciós i entretengut, on se referixen les festes celebrades en la ciutat de València a la proclamació de son rei i senyor nòstron Fernando Sext, que Déu guard, en los dies 19, 20 i 21 d'agost del present any 1746 : Edició crítica i estudi

OpenAIRE

Llop Díaz-Cano, Miquel M.

2014-01-01

En aquest treball es presenta una edició crítica del col·loqui sobre la proclamació del rei Ferran VI. També s'explica la relació dels col·loquis i el teatre breu, temes, llocs de representació, destinatari final i la funció dels seus creadors i recitadors anomenats col·loquiers. A més, s'explica el context sociopolític en què es varen concebre i unes pinzellades sobre el seu autor i la seua producció literària en català. En este trabajo se presenta una edición crítica del coloquio sobre l...

Osteogenesis imperfecta with ectopic mineralizations in dentin and cementum and a COL1A2 mutation.

Science.gov (United States)

Kantaputra, Piranit Nik; Sirirungruangsarn, Yuddhasert; Intachai, Worrachet; Ngamphiw, Chumpol; Tongsima, Sissades; Dejkhamron, Prapai

2018-04-10

We report a Thai father (patient 1) and his daughter (patient 2) affected with osteogenesis imperfecta type IV and dentinogenesis imperfecta. Both were heterozygous for the c.1451G>A (p.Gly484Glu) mutation in COL1A2. The father, a Thai boxer, had very mild osteogenesis imperfecta with no history of low-trauma bone fractures. Scanning electron micrography of the primary teeth with DI of the patient 2, and the primary teeth with DI of another OI patient with OI showed newly recognized dental manifestations of teeth with DI. Normal dentin and cementum might have small areas of ectopic mineralizations. Teeth affected with DI have well-organized ectopic mineralizations in dentin and cementum. The "French-fries-appearance" of the crystals at the cemento-dentinal junction and abnormal cementum have never been reported to be associated with dentinogenesis imperfecta, either isolated or osteogenesis imperfecta-associated. Our study shows for the first time that abnormal collagen fibers can lead to ectopic mineralization in dentin and cementum and abnormal cementum can be a part of osteogenesis imperfecta.

Consumo alimentar de adolescentes matriculados em um colégio particular de Teresina, Piauí, Brasil

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CARVALHO Cecilia Maria Resende Gonçalves de

2001-01-01

Full Text Available Com o objetivo de estudar o consumo alimentar de adolescentes matriculados em um colégio particular de Teresina, realizou-se estudo transversal através de amostragem sistemática. A freqüência de consumo de alimentos expressa em dias por semana foi obtida mediante questionário administrado individualmente ao aluno. O estado nutricional foi avaliado pelo Índice de Massa Corporal, considerando com sobrepeso valores > ou = P85. Os resultados indicam prevalência elevada de risco para o sobrepeso (19,8% nos 334 adolescentes. Os meninos consumiram com maior freqüência alimentos energéticos (milho, manteiga, pizza, lasanha, sucos industrializados, refrigerantes e construtores (frango, ovos, leguminosas em relação às meninas (p < 0,05. Os adolescentes de 10 a 13 anos consumiram com maior freqüência milho, mel, açúcar, rapadura enquanto aqueles com idade entre 17 e 19 anos consumiram mais arroz, bolos, biscoitos, rosca, balas, goma de mascar, refrigerantes, frutas verde-amarelo.

The University of Washington Mobile Planetarium: A Do-it-yourself Guide

Science.gov (United States)

Rosenfield, P.; Gaily, J.; Fraser, O.; Wisniewski, J.

2014-07-01

The University of Washington mobile planetarium project is a student-driven effort to bring astronomy to secondary schools, and the community, in Seattle, USA. This paper presents the solution that was designed and built in order to use the World- Wide Telescope — a computer program created by Microsoft that displays the astronomical sky as maps, the 3D Universe, and earth science data — from a laptop and an off-the-shelf high-definition (HD) projector located in an inflatable plane- tarium. In the first six months of operation, undergraduates at the University of Washington presented planetarium shows to over 1500 people, and 150 secondary school students created and presented their own astronomy projects in our dome, at their school. This paper aims to share the technical aspects of the project so that others can replicate the model or adapt it to their needs. This project was made possible thanks to a NASA/ESA Hubble Space Telescope education/public outreach grant.

Washington Accelerator Conference

International Nuclear Information System (INIS)

Anon.

1993-01-01

Highlights of the 1993 Particle Accelerator Conference, held in Washington in May, were picked out in the previous issue (page 18). Talks on the big hadron colliders reflected the sea-change in the accelerator world where the scale, complexity and cost of the front-line projects has slowed the pace of developments (not unlike the scene in particle physics itself). Speaking before the anti-SSC vote in the House of Representatives in June, Dick Briggs reviewed the situation at the SSC Superconducting Supercollider in Ellis County, Texas. The linac building is near completion and the Low Energy Booster will be ready to receive components early next year. Tunnelling for the Main Ring is advancing rapidly with four boring machines in action. Five miles of tunnel have been completed since January and the pace has now stepped up to nearly a mile each week. The superconducting magnet news is good. Following the successful initial string test of a half cell of the magnet lattice, a two-ring full cell with all associated services is being assembled. The mechanical robustness of the magnet design was confirmed when a dipole was taken to 9.7 T when cooled to 1.8 K. In the Magnet Test Lab itself, ten test stands are installed and equipped

Washington Accelerator Conference

Energy Technology Data Exchange (ETDEWEB)

Anon.

1993-09-15

Highlights of the 1993 Particle Accelerator Conference, held in Washington in May, were picked out in the previous issue (page 18). Talks on the big hadron colliders reflected the sea-change in the accelerator world where the scale, complexity and cost of the front-line projects has slowed the pace of developments (not unlike the scene in particle physics itself). Speaking before the anti-SSC vote in the House of Representatives in June, Dick Briggs reviewed the situation at the SSC Superconducting Supercollider in Ellis County, Texas. The linac building is near completion and the Low Energy Booster will be ready to receive components early next year. Tunnelling for the Main Ring is advancing rapidly with four boring machines in action. Five miles of tunnel have been completed since January and the pace has now stepped up to nearly a mile each week. The superconducting magnet news is good. Following the successful initial string test of a half cell of the magnet lattice, a two-ring full cell with all associated services is being assembled. The mechanical robustness of the magnet design was confirmed when a dipole was taken to 9.7 T when cooled to 1.8 K. In the Magnet Test Lab itself, ten test stands are installed and equipped.

Partners In Motion And Customer Satisfaction In The Washington Dc Metropolitan Area

Science.gov (United States)

1999-06-01

PARTNERS IN MOTION IS A PROGRAM AIMED AT IMPROVING THE QUALITY, QUANTITY, AND AVAILABILITY OF TRAVEL INFORMATION TO TRANSPORTATION AGENCIES, THE MEDIA, AND, ULTIMATELY, TO THE TRAVELER IN THE WASHINGTON, D.C. METROPOLITAN AREA. THE PROGRAM WAS INITIA...

Urban and community forests of the Pacific region: California, Oregon, Washington

Science.gov (United States)

David J. Nowak; Eric J. Greenfield

2010-01-01

This report details how land cover and urbanization vary within the states of California, Oregon, and Washington by community (incorporated and census designated places), county subdivision, and county. Specifically this report provides critical urban and community forestry information for each state including human population characteristics and trends, changes in...

Safety evaluation report related to the operation of WPPSS Nuclear Project No. 2, Docket No. 50-397, Washington Public Power Supply System

International Nuclear Information System (INIS)

1982-12-01

Supplement 2 to the Safety Evaluation Report for Washington Public Power Supply System's application for a license to operate WNP-2 (Docket No. 50-397), located in Benton County, Washington, approximately 12 miles north of Richland, Washington, has been prepared by the Office of Nuclear Reactor Regulation of the US Nuclear Regulatory Commission. This supplement reports the status of certain items that had not been resolved at the time of publication of the Safety Evaluation Report and Supplement 1

Social Vulnerability Index (SoVI) for Washington based on 2000 Census Block Groups

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This data depicts the social vulnerability of Washington census block groups to environmental hazards. Data were culled primarily from the 2000 Decennial Census.

Preliminary assessment report for Kent National Guard Facility (Installation 53065), 24410 Military Road, Kent, Washington

International Nuclear Information System (INIS)

Ketels, P.; Aggarwal, P.; Rose, C.M.

1993-08-01

This report presents the results of the preliminary assessment (PA) conducted by Argonne National Laboratory at the Washington Army National Guard property in Kent, Washington. Preliminary assessments of federal facilities are being conducted to compile the information necessary for completing preremedial activities and to provide a basis for establishing corrective actions in response to releases of hazardous substances. The principal objective of the PA is to characterize the site accurately and determine the need for further action by examining site activities, quantities of hazardous substances present, and potential pathways by which contamination could affect public health and the environment

Osteogenesis imperfecta type I: second-trimester diagnosis and incidental identification of a dominant COL1A1 deletion mutation in the paucisymptomatic father.

Science.gov (United States)

Chen, Chih-Ping; Su, Yi-Ning; Chang, Tung-Yao; Chern, Schu-Rern; Chen, Chen-Yu; Su, Jun-Wei; Wang, Wayseen

2012-06-01

To present second-trimester ultrasound and molecular diagnosis for osteogenesis imperfecta (OI) type I in a female fetus and incidental identification of a dominant COL1A1 deletion mutation in her paucisymptomatic father. A 30-year-old, primigravid woman was referred for genetic counseling in the second trimester because of bowing of the fetal lower limbs. She and her husband were non-consanguineous, and there was no family history of skeletal dysplasias. Prenatal ultrasound at 22 weeks of gestation revealed short and curved right femur and left tibia, and a short left fibula. The lengths of other long bones were normal. The husband was 158 cm tall, had blue sclerae, a history of habitual subluxation and dislocation of bilateral elbows and left knee, and an episode of left ulna fracture, and was not aware of his being affected with OI type I. The woman underwent amniocentesis. Cytogenetic analysis revealed a karyotype of 46,XX. Molecular analysis of the amniocytes revealed a heterozygous deletion mutation of c.1064_1068delCTGGT in exon 17 of the COL1A1 gene. By genetic testing the husband was found to carry the same mutation. Despite counseling of favorable outcome for OI type I with the parents, the woman elected to terminate the pregnancy. Postnatal skeletal X-ray findings were consistent with OI type I. Prenatal ultrasound diagnosis of mild forms of OI should include molecular analysis of type I collagen genes in both fetus and parents. Molecular genetic analysis of the family may incidentally identify a collagen gene mutation in the paucisymptomatic affected parent. Copyright © 2012. Published by Elsevier B.V.

Climate change vulnerability and adaptation in the North Cascades region, Washington

Science.gov (United States)

Crystal L. Raymond; David L. Peterson; Regina M. Rochefort

2014-01-01

The North Cascadia Adaptation Partnership (NCAP) is a science-management partnership consisting of the U.S. Department of Agriculture Forest Service Mount Baker-Snoqualmie and Okanogan-Wenatchee National Forests and Pacific Northwest Research Station; North Cascades National Park Complex; Mount Rainier National Park; and University of Washington Climate Impacts Group....

78 FR 37104 - Establishment of Area Navigation (RNAV) Routes; Washington, DC

Science.gov (United States)

2013-06-20

...-0081; Airspace Docket No. 12-AEA-5] RIN 2120-AA66 Establishment of Area Navigation (RNAV) Routes... Register approves this incorporation by reference action under 1 CFR part 51, subject to the annual... establishing five RNAV routes in the Washington, DC area (78 FR 29615). Subsequent to publication, it was...

Accounting Manual for Educational Service Districts in the State of Washington

Science.gov (United States)

Lunghofer, Daniel S.

2010-01-01

This manual provides principles that are an integral part of the accounting requirements for educational service districts (ESDs) in the state of Washington. They are in conformance with generally accepted accounting principles, except where referenced in the ESD principles that follow. This manual provides guidance on the following topics:(1)…

Quality-assurance plan for groundwater activities, U.S. Geological Survey, Washington Water Science Center

Science.gov (United States)

Kozar, Mark D.; Kahle, Sue C.

2013-01-01

This report documents the standard procedures, policies, and field methods used by the U.S. Geological Survey’s (USGS) Washington Water Science Center staff for activities related to the collection, processing, analysis, storage, and publication of groundwater data. This groundwater quality-assurance plan changes through time to accommodate new methods and requirements developed by the Washington Water Science Center and the USGS Office of Groundwater. The plan is based largely on requirements and guidelines provided by the USGS Office of Groundwater, or the USGS Water Mission Area. Regular updates to this plan represent an integral part of the quality-assurance process. Because numerous policy memoranda have been issued by the Office of Groundwater since the previous groundwater quality assurance plan was written, this report is a substantial revision of the previous report, supplants it, and contains significant additional policies not covered in the previous report. This updated plan includes information related to the organization and responsibilities of USGS Washington Water Science Center staff, training, safety, project proposal development, project review procedures, data collection activities, data processing activities, report review procedures, and archiving of field data and interpretative information pertaining to groundwater flow models, borehole aquifer tests, and aquifer tests. Important updates from the previous groundwater quality assurance plan include: (1) procedures for documenting and archiving of groundwater flow models; (2) revisions to procedures and policies for the creation of sites in the Groundwater Site Inventory database; (3) adoption of new water-level forms to be used within the USGS Washington Water Science Center; (4) procedures for future creation of borehole geophysics, surface geophysics, and aquifer-test archives; and (5) use of the USGS Multi Optional Network Key Entry System software for entry of routine water-level data

México, ejemplo de las politicas anti-desarrollo del Consenso de Washington

Directory of Open Access Journals (Sweden)

Arturo Guillén

2012-08-01

Full Text Available A partir de los avances logrados por el "nuevo-desarrollismo", examina las políticas macroeconómicas implementadas en México en el contexto del Consenso de Washington, la cuales en el caso mexicano fueron institucionalizadas con la firma del Tratado de Libre Comercio de América del Norte (TLCAN. Este tratado consolidó el modelo neoliberal y aherrojó la suerte de la economía mexicana al desempeño de la economía de Estados Unidos. Se analiza la economía mexicana como un caso paradigmático de los efectos anti-crecimiento, de desarticulación de los sistemas productivos y desestabilizadores de las políticas procíclicas inspiradas en el Consenso de Washington. Se concluye que los principales obstáculos para implementar una estrategia alternativa de desarrollo en México, orientada al crecimiento sustentable con equidad, son políticos y residen en la dominación del poder político por una oligarquía financiera rentista que carece de todo idea de proyecto nacional,A partir dos avanços conseguidos pelo "novo-desenvolvimentismo", o artigo examina as políticas macroeconômicas implementadas no México no contexto do Consenso de Washington, as quais, no caso mexicano, foram institucionalizadas com a assinatura do Tratado de Livre Comércio de América do Norte (TLCAN. Este tratado consolidou o modelo neoliberal e agrilhoou a sorte da economia mexicana ao desempenho da economia dos Estados Unidos. No artigo, a economia mexicana analisa-se como um caso paradigmático dos efeitos anticrescimento, de desarticulação dos sistemas produtivos e desestabilizadores das políticas pró-cíclicas inspiradas no Consenso de Washington. Conclui-se que os principais obstáculos para levar à prática uma estratégia alternativa de desenvolvimento no México, orientada ao crescimento sustentável com equidade, são políticos e residem na dominação do poder político por uma oligarquia financeira rentista que carece de qualquer ideia de projeto

Analyzing job entry examinations for Mathematics teachers of Colegio Pedro II during the 19th Century Analisando provas de concursos para professores de Matemática no Colégio Pedro II no século XIX

Directory of Open Access Journals (Sweden)

Flávia Soares

2010-06-01

Full Text Available In a attempt to investigate historic aspects of mathematics teaching, we ask what kind of instruments can be used to rebuild the professional trajectory of teachers and their practices, and what elements can be used to analyze the issues related to promotion of mathematics teacher in Brazil. One of these elements is public teacher job entry examinations. The analysis of these exams can show what were the requirements to work as a teacher and to clear other important questions about the conception about teachers at that time, about the contents they should teach, and others. This paper emphasizes the contents of the exams, and the formal requirements and abilities measured in those exams to act as a mathematics teacher in an important secondary education institution in the 19th Century, Colégio Pedro II. Keywords: Public contest. Mathematics teachers. Century XIX. Colégio Pedro II.Na tentativa de investigar aspectos históricos do ensino de Matemática, questionam-se que tipos de instrumentos podem ser utilizados para reconstruir a trajetória profissional do professor e de suas práticas e a partir de quais elementos pode-se examinar de maneira mais rigorosa as questões relativas ao acesso à profissão de professor de Matemática no Brasil. Um desses elementos são as provas de concursos. A análise dessas provas é capaz de revelar quais as exigências para o exercício do magistério e trazer à tona questões importantes a respeito da concepção da época em relação ao papel do professor, aos conteúdos que deveria ensinar, entre outros aspectos. Este trabalho enfatiza o conteúdo das provas, as exigências formais e as habilidades medidas nos exames para os cargos de professor de Matemática da principal instituição de ensino secundário do Brasil no século XIX, o Colégio Pedro II. Palavras-chave: Concursos. Professor de Matemática. Século XIX. Colégio Pedro II.

Aerial Photography and Imagery, Ortho-Corrected, Washington County, NC true color orthophotography - 1/4 foot resolution over selected areas, Published in 2009, 1:1200 (1in=100ft) scale, Washington County Government.

Data.gov (United States)

NSGIC Local Govt | GIS Inventory — Aerial Photography and Imagery, Ortho-Corrected dataset current as of 2009. Washington County, NC true color orthophotography - 1/4 foot resolution over selected...

Influence of arterial hypertension on colonic healing in rats Influência da hipertensão arterial na cicatrização colônica em ratos

Directory of Open Access Journals (Sweden)

Gilberto Luiz Ortolan

2012-08-01

Full Text Available PURPOSE: Evaluation of colonic healing in spontaneously hypertensive rats. METHODS: Fifty male, young and inbred rats were used. Twenty-five Wistar Kyoto rats (WKY as control and twenty-five spontaneously hypertensive rats (SHR as an experimental group. Colotomy and bowel suture at 2.5 cm from the peritoneal reflection were performed. All animals were allocated randomly into sub-groups for review at the third, seventh and fourteenth days after surgery. We evaluated the concentration of angiotensin II, the burst pressure, epithelialization, the organization of the tunics of the bowel wall, inflammatory response and collagen deposition. RESULTS: The burst pressure, epithelialization, organization of the tunics and collagen deposition was not significant between groups. The inflammatory reaction was more intense in the control group on the third postoperative day (p=0.023 as the experimental group on the remaining time. CONCLUSION: Systemic arterial hypertension in rats did not influence significantly the healing process of colonic anastomoses.OBJETIVO: Avaliar a cicatrização colônica em ratos espontaneamente hipertensos. MÉTODOS: Cinquenta ratos machos, jovens e isogênicos. Vinte e cinco ratos da linhagem Wistar Kyoto (WKY como controle e vinte e cinco ratos espontaneamente hipertensos (SHR como grupo experimento. Realizou-se colotomia e colorrafia a 2,5 cm da reflexão peritoneal. Alocaram-se os animais aleatoriamente em sub-grupos para avaliação no terceiro, sétimo e décimo quarto dias de pós-operatório. Foram avaliados a concentração de angiotensina II, a resistência da anastomose à insuflação, a epitelização, a organização das túnicas da parede intestinal, a reação inflamatória e a deposição de colágeno. RESULTADOS: A avaliação da resistência da anastomose, epitelização, organização das túnicas e deposição de colágeno não foi significativa entre os grupos. A reação inflamatória foi mais intensa no

Washington's marine oil spill compensation schedule - simplified resource damage assessment

International Nuclear Information System (INIS)

Geselbracht, L.; Logan, R.

1993-01-01

The Washington State Preassessment Screening and Oil Spill Compensation Schedule Rule (Chapter 173-183 Washington Administrative Code), which simplifies natural resource damage assessment for many oil spill cases, became effective in May 1992. The approach described in the rule incorporates a number of preconstructed rankings that rate environmental sensitivity and the propensity of spilled oil to cause environmental harm. The rule also provides guidance regarding how damages calculated under the schedule should be reduced to take into account actions taken by the responsible party that reduce environmental injury. To apply the compensation schedule to marine estuarine spills, the resource trustees need only collect a limited amount of information such as type of product spilled, number of gallons spilled, compensation schedule subregions the spill entered, season of greatest spill impact, percent coverage of habitats affected by the spill, and actions taken by the responsible party. The result of adding a simplified tool to the existing assortment of damage assessment approaches is that resource trustees will now be able to assess damages for most oil spill cases and shift more effort than was possible in the past to resource restoration

Washington State Need Grant: Less-Than-Halftime Pilot Project (SHB 1345)

Science.gov (United States)

Washington Higher Education Coordinating Board, 2006

2006-01-01

The 2005 Washington State Legislature authorized, through Substitute House Bill 1345, a two-year pilot project allowing eligible students, who enroll for four or five credits in a term, to receive the State Need Grant (SNG). Several important policy considerations emerged during the pilot project. Board staff explored these issues with financial…

Timber resource statistics for nonnational forest land in western Washington, 2001.

Science.gov (United States)

Andrew N. Gray; Charles F. Veneklase; Robert D. Rhoads

2005-01-01

This report is a summary of timber resource statistics for an inventory of the 19 counties in western Washington: Clallam, Clark, Cowlitz, Grays Harbor, Island, Jefferson, King, Kitsap, Lewis, Mason, Pacific, Pierce, San Juan, Skagit, Skamania, Snohomish, Thurston, Wahkiakum, and Whatcom. The inventory in 2000 sampled all private and public lands except those...

Residents' attitudes toward tourism development: a case study of Washington, NC

Science.gov (United States)

Yasong Wang; Robert E. Pfister; Duarte B. Morais

2007-01-01

This study examined the relationship between socio-economic and demographic attributes of local residents and their attitudes toward tourism in Washington, NC, a small community where tourism is in its development stage. Residents' attitudes toward tourism were measured by adapting 20 items from the Tourism Impact Attitude Scale developed by Lankford and Howard (...

Now It's Necessary: Virtual Reference Services at Washington State University, Pullman

Science.gov (United States)

Nicol, Erica Carlson; Crook, Linda

2013-01-01

While virtual reference services (VRS) are becoming more and more common in academic libraries, implementing and maintaining well-used and effective VRS can be a challenge in the face of competing demands on time, staffing, and funding. Between 2011 and 2012, librarians at Washington State University, Pullman (WSU) have overhauled and reorganized…

Study of the Incentive Program for Washington's National Board Certified Teachers

Science.gov (United States)

Plecki, Margaret L.; Elfers, Ana M.; St. John, Elise; Finster, Matthew; Emry, Terese; Nishida, Nasue; Harmon, Jeanne

2010-01-01

This study examines the impact of Washington state's incentives for teachers to attain National Board Certification and to work in challenging schools. Using surveys and secondary analyses of state databases, we examine the workforce both prior to and following recent changes in the incentive program. The study considers the nature of National…